Tank 241-SY-102 January 2000 Compatibility Grab Samples Analytical Results for the Final Report [SEC 1 and 2

Energy Technology Data Exchange (ETDEWEB)

BELL, K.E.

2000-05-11

This document is the format IV, final report for the tank 241-SY-102 (SY-102) grab samples taken in January 2000 to address waste compatibility concerns. Chemical, radiochemical, and physical analyses on the tank SY-102 samples were performed as directed in Comparability Grab Sampling and Analysis Plan for Fiscal Year 2000 (Sasaki 1999). No notification limits were exceeded. Preliminary data on samples 2SY-99-5, -6, and -7 were reported in ''Format II Report on Tank 241-SY-102 Waste Compatibility Grab Samples Taken in January 2000'' (Lockrem 2000). The data presented here represent the final results.

Hazard evaluation for transfer of waste from tank 241-SY-101 to tank 241-SY-102

International Nuclear Information System (INIS)

Shultz, M.V.

1999-01-01

Tank 241-SY-101 waste level growth is an emergent, high priority issue. The purpose of this document is to record the hazards evaluation process and document potential hazardous conditions that could lead to the release of radiological and toxicological material from the proposed transfer of a limited quantity (approximately 100,000 gallons) of waste from Tank 241-SY-101 to Tank 241-SY-102. The results of the hazards evaluation were compared to the current Tank Waste Remediation System (TWRS) Basis for Interim Operation (HNF-SD-WM-BIO-001, 1998, Revision 1) to identify any hazardous conditions where Authorization Basis (AB) controls may not be sufficient or may not exist. Comparison to LA-UR-92-3196, A Safety Assessment for Proposed Pump Mixing Operations to Mitigate Episodic Gas Releases in Tank 241-SY-101, was also made in the case of transfer pump removal activities. Revision 1 of this document deletes hazardous conditions no longer applicable to the current waste transfer design and incorporates hazardous conditions related to the use of an above ground pump pit and overground transfer line. This document is not part of the AB and is not a vehicle for requesting authorization of the activity; it is only intended to provide information about the hazardous conditions associated with this activity. The AB Control Decision process will be used to determine the adequacy of controls and whether the proposed activity is within the AB. This hazard evaluation does not constitute an accident analysis

Desulfurization of dibenzothiophene by Corynebacterium sp. strain SY1

International Nuclear Information System (INIS)

Omori, Toshio; Monna, L.; Saiki, Yuko; Kodama, Tohru

1992-01-01

Strain SY1, identified as a Corynebacterium sp., was isolated on the basis of the ability to utilize dibenzothiophene (DBT) as a sole source of sulfur. Strain SY1 could utilize a wide range of organic and inorganic sulfur compounds, such as DBT sulfone, dimethyl sulfide, dimethyl sulfoxide, dimethyl sulfone, CS 2 , FeS 2 , and even elemental sulfur. Strain SY1 metabolized DBT to dibenzothiophene-5-oxide, DBT sulfone, and 2-hydroxybiphenyl, which was subsequently nitrated to produce at least two different hydroxynitrobiphenyls during cultivation. These metabolites were separated by silica gel column chromatography and identified by nuclear magnetic resonance, UV, and mass spectral techniques. Resting cells of SY1 desulfurized toluenesulfonic acid and released sulfite anion. On the basis of these results, a new DBT degradation pathway is proposed

Toimintaterapian mahdollisuudet syöpätautien vuodeosastoilla

OpenAIRE

Nurmi, Mirka; Ohlgren, Sanna; Välimäki, Petra

2011-01-01

Syöpäpotilaiden määrä on kasvanut jatkuvasti ja tulee lisääntymään väistämättä väestön ikääntyessä. On arvioitu, että Suomessa syöpään sairastuu vuonna 2020 yli 33 000 ihmistä, joka on 27 % enemmän kuin vuonna 2008. Mahdollisuus toimia on yksi ihmisen perustarpeista ja syöpään sairastuminen vaikuttaa ihmisen toiminnallisuuteen monilla eri tavoilla. Sairastuminen tai vammautuminen saattaa johtaa tilanteeseen, jossa ihmisen on mahdotonta suoriutua arvostamistaan toiminnoista omassa elämässään. ...

241-SY Tank Farm Construction Extent of Condition Review for Tank Integrity

Energy Technology Data Exchange (ETDEWEB)

Barnes, Travis J.; Boomer, Kayle D.; Gunter, Jason R.; Venetz, Theodore J.

2013-07-25

This report provides the results of an extent of condition construction history review for tanks 241-SY-101, 241-SY-102, and 241-SY-103. The construction history of the 241-SY tank farm has been reviewed to identify issues similar to those experienced during tank 241-AY-102 construction. Those issues and others impacting integrity are discussed based on information found in available construction records, using tank 241-AY-102 as the comparison benchmark. In the 241-SY tank farm, the third DST farm constructed, refractory quality and stress relief were improved, while similar tank and liner fabrication issues remained.

Tank SY-101 void fraction instrument functional design criteria

International Nuclear Information System (INIS)

McWethy, L.M.

1994-01-01

This document presents the functional design criteria for design, analysis, fabrication, testing, and installation of a void fraction instrument for Tank SY-101. This instrument will measure the void fraction in the waste in Tank SY-101 at various elevations

Hanford's Battle with Nuclear Waste Tank SY-101: Bubbles, Toils, and Troubles

International Nuclear Information System (INIS)

Stewart, Charles W.

2006-01-01

people, from pervasive misconception to grand insight, near miss to sweeping success, meddling interference to close teamwork, all on an uncommonly large scale. It was a necessary catharsis that transformed the entire Hanford culture from a closed defense production operation to an open environmental cleanup project. Its tight project discipline and close teamwork became the Hanford standard. The final remediation of SY-101 placed second in an international ''project of the year'' competition. Many consider SY-101 work the peak of their careers and measure all other experience by it. SY-101 defines some of the worst and the best of Hanford history. This book attempts to narrate and explain the whole vast story

241-SY-101 mixer pump lifetime expectancy. Final report

International Nuclear Information System (INIS)

Shaw, C.P.

1995-01-01

The purpose of WHC-SD-WM-TI-726, Rev. 0 241-SY-101 Mixer Pump Lifetime Expectancy is to determine a best estimate of the mean lifetime of non-repairable (located in the waste) essential features of the hydrogen mitigation mixer pump presently installed in 101-SY. The estimated mean lifetime is 9.1 years. This report does not demonstrate operation of the entire pump assembly within the Tank Farm ''safety envelope''. It was recognized by the Defense Nuclear Facilities Safety Board (DNFSB) this test pump was not specifically designed for long term service in tank 101-SY. In June 95 the DNFSB visited Hanford and ask the question, ''how long will this test pump last and how will the essential features fail?'' During the 2 day meeting with the DNFSB it was discussed and defined within the meeting just exactly what essential features of the pump must operate. These essential features would allow the pump to operate for the purpose of extending the window for replacement. Operating with only essential features would definitely be outside the operating safety envelope and would require a waiver. There are three essential features: 1. The pump itself (i.e. the impeller and motor) must operate 2. Nozzles and discharges leg must remain unplugged 3. The pump can be re-aimed, new waste targeted, even if manually

ECTA/DaSy Framework Self-Assessment Comparison Tool

Science.gov (United States)

Center for IDEA Early Childhood Data Systems (DaSy), 2016

2016-01-01

The Self-Assessment Comparison (SAC) Tool is for state Part C and Section 619/Preschool programs to use to assess changes in the implementation of one or more components of the ECTA System Framework and/or subcomponenets of the DaSy Data System Framework. It is a companion to the ECTA/DaSy Framework Self-Assessment. Key features of the SAC are…

Process Control Plan for Tank 241-SY-101 Surface Level Rise Remediation

International Nuclear Information System (INIS)

ESTEY, S.D.

1999-01-01

The tank 241-SY-101 transfer system was conceived and designed to address the immediate needs presented by rapidly changing waste conditions in tank 241-SY-101. Within the last year or so, the waste in this tank has exhibited unexpected behavior (Rassat et al. 1999) in the form of rapidly increasing crust growth. This growth has been brought about by a rapidly increasing rate of gas entrapment within the crust. It has been conceived that the lack of crust agitation beginning upon the advent of mixer pump operations may have set-up a more consolidated, gas impermeable barrier when compared to a crust regularly broken up by the prior buoyant displacement events within the tank. As a result, a series of level-growth remediation activities have been developed for tank 241-SY-101. The initial activities are also known as near-term crust mitigation. The first activity of near-term mitigation is to perform the small transfer of convective waste from tank 241-SY-101 into tank 241-SY-102. A 100 kgal transfer represents about a 10% volume reduction allowing a 10% water in-tank dilution. Current thinking holds that this should be enough to dissolve nitrite solids in the crust and perhaps largely eliminate gas retention problem in the crust (Raymond 1999). Additional mitigation activities are also planned on less constrained schedules. The net affect of the small transfer and follow-on mitigation activities for tank 241-SY-101 is strongly believed to be the remediation of tank 241-SY-101 as a flammable gas safety concern. The process for remediating the tank will require two or more transfer/dilution cycles. In-tank dilution will begin shortly after the initial transfer and the total dilution required to reach the final state is estimated to be between 250 to 400K gallons. The final state of the waste will not require any active measures to safely store the waste and operation of the mixer pump will no longer be necessary. The remediation activities are centered on a purpose

Status of tank 241-SY-101 data analyses

International Nuclear Information System (INIS)

Anantatmula, R.P.

1992-09-01

The Waste Tank Flammable Gas Stabilization Program was established in 1990 to provide for resolution of a major safety issue identified for 23 of the high-level waste tanks at the Hanford Site. The safety issue involves the production, accumulation, and periodic release from these tanks of flammable gases in concentrations exceeding the lower flammability limits. This document deals primarily with tank 241-SY-101 from the SY Tank Farm. The flammable gas condition has existed for this tank since the tank was first filled in the time period from 1977 to 1980. During a general review of waste tank chemical stability in 1988--1989, this situation was re-examined and, in March 1990, the condition was declared to be an unreviewed safety question. Tank 241-SY-101 was placed under special operating restrictions, and a program of investigation was begun to evaluate the condition and determine appropriate courses of action. This report summarizes the data that have become available on tank 241-SY-101 since it was declared as an unreviewed safety question and updates the information reported in an earlier document (WHC-EP-0517). The report provides a technical basis for use in the evaluation of safety risks of the tank and subsequent resolution of the unreviewed safety question

Engineering test plan for Tank 241-SY-101 in situ viscometer

International Nuclear Information System (INIS)

Sobocinski, R.G.; Stokes, T.I.; Pearce, K.L.

1994-11-01

To obtain in situ measurements of the rheological properties within tank 241-SY-101, this document will implement the test strategy defined in PNLMIT-041994, acquisition and Reduction of Data Obtained in Tank SY-101 with the Ball Rheometer. Instructions for all sequences are defined within the procedure. All safety requirements as defined in LA-UR-92-3196, A Safety Assessment for Proposed Mixing Operations to Mitigate Episodic Gas Releases in Tank 241-101-SY have been implemented into this procedure

Acceptance Test Report for 241-SY Pump Cradle Hydraulic System

International Nuclear Information System (INIS)

Koons, B.M.

1995-01-01

The purpose of this ATP is to verify that hydraulic system/cylinder procured to replace the cable/winch system on the 101-SY Mitigation Pump cradle assembly fulfills its functional requirements for raising and lowering the cradle assembly between 70 and 90 degrees, both with and without pump. A system design review was performed on the 101-SY Cradle Hydraulic System by the vendor before shipping (See WHC-SD-WM-DRR-045, 241-SY-101 Cradle Hydraulic System Design Review). The scope of this plan focuses on verification of the systems ability to rotate the cradle assembly and any load through the required range of motion

Syed Mahmood Naqvi (27 August 1941 - 04 September 2009)

Digital Repository Service at National Institute of Oceanography (India)

Naqvi, S.W.A.; Rao, Y.J.B.

-1 SYED MAHMOOD NAQVI (1941-2009) Elected Fellow 1986 LIFE, EDUCATION AND FAMILY Being a descendent of Prophet Muhammad's daughter Syeda (hence the title 'Syed') through the 10 lh Shia Imam Ali Naqi (hence the surname 'Naqvi'). Mahmood's roots into science... living, something that he was not used to. He was only moderately educated. However, both he and his father wanted Bhni to obtain ...

Syed Manzoorul Islam’s Postmodern Tales: A Study

Directory of Open Access Journals (Sweden)

Md Abdul Momen Sarker

2016-10-01

Full Text Available The paper brings into focus how Syed Manzoorul Islam, in his three-decade-long literary career, has mastered a narrative style that sets him apart from many of his Bengali contemporaries. It demonstrates all the traits unique to his storytelling: blurring of boundaries between dream and reality, self-reflexivity, irony, and humor. The research also encapsulates how Syed Islam is different from his contemporary short story writers in terms of constructing plot and character. It foregrounds the author’s capability of developing a diction which is completely his own. The paper discusses the postmodern features prevailing in his stories. It shows us how the author invites the readers to be a part of his discourses. It summarizes the author’s surrealist imagination which creates a world that is strangely familiar and unfamiliar at the same time. Overall, the research analyzes how the postmodern elements relate to the major themes of Syed Manzoorul Islam’s short stories. Keywords: Post-modernism, magic-realism, realism, psychoanalysis, political degeneration

The effect of dilution on the gas retention behavior of Tank 241-SY- 103 waste

International Nuclear Information System (INIS)

Bredt, P.R.; Tingey, S.M.

1996-01-01

Twenty-five of the 177 underground waste storage tanks on the Hanford Site have been placed on the Flammable Gas watch list. These 25 tanks, containing high-level waste generated during plutonium and uranium processing, have been identified as potentially capable of accumulating flammable gases above the lower flammability limit (Babad et al. 1991). In the case of Tanks 241-SY-101 and 241-SY-103, it has been proposed that diluting the tank waste may mitigate this hazard (Hudson et al. 1995; Stewart et al. 1994). The effect of dilution on the ability of waste from Tank 241-SY-103 to accumulate gas was studied at Pacific Northwest National Laboratory. A similar study has been completed for waste from Tank 241-SY-101 (Bredt et al. 1995). Because of the additional waste-storage volume available in Tank 241-SY-103 and because the waste is assumed to be similar to that currently in Tank 241-SY-101, Tank 241-SY-103 became the target for a demonstration of passive mitigation through in-tank dilution. In 1994, plans for the in-tank dilution demonstration were deferred pending a decision on whether to pursue dilution as a mitigation strategy. However, because Tank 241-SY-103 is an early retrieval target, determination of how waste properties vary with dilution will still be required

Design review report for the SY-101 RAPID mitigation system

International Nuclear Information System (INIS)

SCHLOSSER, R.L.

1999-01-01

This report documents design reviews conducted of the SY-101 Respond And Pump In Days (RAPID) Mitigation System. As part of the SY-101 Surface-Level-Rise Remediation Project, the SY-101 WID Mitigation System will reduce the potential unacceptable consequences of crust growth in Tank 241-SY-101 (SY-101). Projections of the crust growth rate indicate that the waste level in the tank may reach the juncture of the primary and secondary confinement structures of the tank late in 1999. Because of this time constraint, many design activities are being conducted in parallel and design reviews were conducted for system adequacy as well as design implementation throughout the process. Design implementation, as used in this design review report, is the final component selection (e.g., which circuit breaker, valve, or thermocouple) that meets the approved design requirements, system design, and design and procurement specifications. Design implementation includes the necessary analysis, testing, verification, and qualification to demonstrate compliance with the system design and design requirements. Design implementation is outside the scope of this design review. The design activities performed prior to detailed design implementation (i.e., system mission requirements, functional design requirements, technical criteria, system conceptual design, and where design and build contracts were placed, the procurement specification) have been reviewed and are within the scope of this design review report. Detailed design implementation will be controlled, reviewed, and where appropriate, approved in accordance with Tank Waste Remediation System (TWRS) engineering procedures. Review of detailed design implementation will continue until all components necessary to perform the transfer function are installed and tested

Design review report for the SY-101 RAPID mitigation system

Energy Technology Data Exchange (ETDEWEB)

SCHLOSSER, R.L.

1999-05-24

This report documents design reviews conducted of the SY-101 Respond And Pump In Days (RAPID) Mitigation System. As part of the SY-101 Surface-Level-Rise Remediation Project, the SY-101 WID Mitigation System will reduce the potential unacceptable consequences of crust growth in Tank 241-SY-101 (SY-101). Projections of the crust growth rate indicate that the waste level in the tank may reach the juncture of the primary and secondary confinement structures of the tank late in 1999. Because of this time constraint, many design activities are being conducted in parallel and design reviews were conducted for system adequacy as well as design implementation throughout the process. Design implementation, as used in this design review report, is the final component selection (e.g., which circuit breaker, valve, or thermocouple) that meets the approved design requirements, system design, and design and procurement specifications. Design implementation includes the necessary analysis, testing, verification, and qualification to demonstrate compliance with the system design and design requirements. Design implementation is outside the scope of this design review. The design activities performed prior to detailed design implementation (i.e., system mission requirements, functional design requirements, technical criteria, system conceptual design, and where design and build contracts were placed, the procurement specification) have been reviewed and are within the scope of this design review report. Detailed design implementation will be controlled, reviewed, and where appropriate, approved in accordance with Tank Waste Remediation System (TWRS) engineering procedures. Review of detailed design implementation will continue until all components necessary to perform the transfer function are installed and tested.

ATR/OTR-SY Tank Camera Purge System and in Tank Color Video Imaging System

International Nuclear Information System (INIS)

Werry, S.M.

1995-01-01

This procedure will document the satisfactory operation of the 101-SY tank Camera Purge System (CPS) and 101-SY in tank Color Camera Video Imaging System (CCVIS). Included in the CPRS is the nitrogen purging system safety interlock which shuts down all the color video imaging system electronics within the 101-SY tank vapor space during loss of nitrogen purge pressure

Work plan for new SY tank farm exhauster, on-site fabrication activities

International Nuclear Information System (INIS)

McClees, J.

1994-01-01

The replacement SY tank farm exhauster unit is a new piece of equipment, designed to replace the existing SY tank farm K1 Ventilation System exhauster unit. This work plan describes the shop fabrication activities associated with the receiving, assembly, repair, modification, and testing of the new SY tank farm primary exhauster. A general list of these activities include, but are not limited to: repair all shipping damages, including procurement of replacement parts; fabricate hardware needed to install exhauster in the field (e.g., Vent duct tie-in, duct concrete footings/hangers, stack concrete footings, etc.); incorporate equipment modification as provided by WHC Engineering (e.g., Rewire the Alarm Annunciator Cabinet as fail-safe, connections between the exhauster and stack sample cabinet, etc.); test the entire exhauster unit, to the extent possible, prior to field installation; and prepare exhauster unit for transfer to and installation at SY tank farm

9+ The Year-Round School.

Science.gov (United States)

American Association of School Administrators, Washington, DC.

The 9-month school year with a 3-month summer vacation had its origin in our earlier agrarian life. Today's teacher shortages, overcrowded schools, and pressures to learn demand extensions of the school year. This publication analyzes five programs: (1) a staggered-vacation school year for all, (2) a full 48-week school year for all, (3) a…

Documentation to the NCES Common Core of Data Local Education Agency Universe Survey: School Year 2009-10, Version Provisional 2a. NCES 2011-349rev

Science.gov (United States)

Keaton, Patrick; Sable, Jennifer; Liu, Fei

2012-01-01

This revised data file includes corrections that were provided to NCES as a result of a special collection effort designed to address data quality issues found in the 1a release of this file. In May 2012, NCES became aware of data errors for key data items for several schools on the published version of the SY 2009-10 school file; in some cases…

241-SY-101 DACS instrument problem screen (SCR 448) acceptance test procedure

International Nuclear Information System (INIS)

ERMI, A.M.

1999-01-01

The operability of the 241-SY-101 Data Acquisition and Control System (DACS) to provide proper control and monitoring of the mitigation mixer pump and instrumentation installed in the 241-SY-101 underground storage tank utilizing the [INSTPROB] screen will be systematically evaluated by the performance of this procedure

Isolation and characterization of a plant growth-promoting rhizobacterium, Serratia sp. SY5.

Science.gov (United States)

Koo, So-Yeon; Cho, Kyung-Suk

2009-11-01

The role of plant growth-promoting rhizobacteria (PGPR) in the phytoremediation of heavy-metal-contaminated soils is important in overcoming its limitations for field application. A plant growth-promoting rhizobacterium, Serratia sp. SY5, was isolated from the rhizoplane of barnyard grass (Echinochloa crus-galli) grown in petroleum and heavy-metal-contaminated soil. This isolate has shown capacities for indole acetic acid production and siderophores synthesis. Compared with a non-inoculated control, the radicular root growth of Zea mays seedlings inoculated with SY5 can be increased by 27- or 15.4-fold in the presence of 15 mg-Cd/l or 15 mg-Cu/l, respectively. The results from hydroponic cultures showed that inoculation of Serratia sp. SY5 had a favorable influence on the initial shoot growth and biomass of Zea mays under noncontaminated conditions. However, under Cd-contaminated conditions, the inoculation of SY5 significantly increased the root biomass of Zea mays. These results indicate that Serratia sp. SY5 can serve as a promising microbial inoculant for increased plant growth in heavy-metal-contaminated soils to improve the phytoremediation efficiency.

Acceptance test procedure, 241-SY-101 Flexible Receiver System, Phase III testing

International Nuclear Information System (INIS)

Ritter, G.A.

1994-01-01

This Acceptance Test Procedure is for the 241-SY-101 Flexible Receiver System, Phase III Testing. This procedure will test the sealing integrity of the Flexible Receiver System to ensure that release of waste and aerosols will be minimized during the removal of the test mixer pump from tank SY-101

Spectrophotometry of the nucleus of the Sy 2 galaxy Markarian 308

International Nuclear Information System (INIS)

Popov, V.N.; Khachikyan, Eh.E.

1980-01-01

The results of spectrophotometry of the Sy2 galaxy Markarian 308 are presented. The profiles of the emission lines [N II] lambda lambda 6583, 6548 A, Hsub(α), [O III] lambda lambda 5007, 4959 A and Hsub(β) are drawn. The electron density, electron temperature, mass and effective volume of the emitting gas are estimated. Markarian 308 shows now less activity compared with other Sy2 galaxies

Mixer pump long term operations plan for Tank 241-SY-101 mitigation

International Nuclear Information System (INIS)

Irwin, J.J.

1994-01-01

This document provides the general Operations Plan for performance of the mixer pump long term operations for Tank 241-SY-101 mitigation of gas retention and periodic release in Tank 101-SY. This operations plan will utilize a 112 kW (150 hp) mixing pump to agitate/suspend the particulates in the tank

SY Tank Farm ventilation isolation option risk assessment report

Energy Technology Data Exchange (ETDEWEB)

Powers, T.B.; Morales, S.D.

1994-03-01

The safety of the 241-SY Tank Farm ventilation system has been under extensive scrutiny due to safety concerns associated with tank 101-SY. Hydrogen and other gases are generated and trapped in the waste below the liquid surface. Periodically, these gases are released into the dome space and vented through the exhaust system. This attention to the ventilation system has resulted in the development of several alternative ventilation system designs. The ventilation system provides the primary means of mitigation of accidents associated with flammable gases. This report provides an assessment of various alternatives ventilation system designs.

Ion exchange removal of cesium from simulated and actual supernate from Hanford tanks 241-SY-101 and 241-SY-103

International Nuclear Information System (INIS)

Brown, G.N.; Bontha, J.R.; Carlson, C.D.

1995-09-01

Pacific Northwest Laboratory (PNL), in conjunction with the Process Chemistry and Statistics Section of Westinghouse Hanford Company (WHC), conducted this study as part of the Supernatant Treatment Development Task for the Initial Pretreatment Module (IPM) Applied Engineering Project. The study assesses the performance of the CS-100 ion exchange material for removing cesium from simulated and actual alkaline supernate from Hanford tanks 241-SY-101 and 241-SY-103. The objective of these experiments is to compare the cesium ion exchange loading and elution profiles of actual and simulated wastes. Specific experimental objectives include (1) demonstration of decontamination factors (DF) for cesium removal, 92) verification of simulant performance, (3) investigation of waste/exchanger chemistry, and (4) determination of the radionuclide content of the regenerated CS-100 resin prior to disposal

Design Review Closure Report for the SY-101 Rapid Transfer System

International Nuclear Information System (INIS)

POWELL, W.J.

1999-01-01

The purpose of this report, is to document closure of design review open items, resulting from design reviews conducted for the SY-101 Respond And Pump In Days (RAPID) Transfer System. Results of the various design reviews were documented in the Design Review Report for The SY-101 Rapid Mitigation System, HNF-4519. In that report, twenty-three open items were identified. In this report the 23 items are reviewed and statused

Design Review Closure Report for the SY-101 Rapid Transfer System

Energy Technology Data Exchange (ETDEWEB)

POWELL, W.J.

1999-11-29

The purpose of this report, is to document closure of design review open items, resulting from design reviews conducted for the SY-101 Respond And Pump In Days (RAPID) Transfer System. Results of the various design reviews were documented in the Design Review Report for The SY-101 Rapid Mitigation System, HNF-4519. In that report, twenty-three open items were identified. In this report the 23 items are reviewed and statused.

CompTIA Security+ Review Guide Exam SY0-301

CERN Document Server

Stewart, James M

2011-01-01

Reinforce your preparation for CompTIA's new Security+ exam with this focused review tool Before you take CompTIA's new Security+ exam SY0-301, reinforce your learning with a thorough review and lots of practice. The new edition of this concise guide helps you do just that. It covers all six domains of exam SY0-301, all exam objectives, and includes a helpful "Exam Essentials" section after each domain to help you zero in on what you need to know for the exam. A companion CD offers additional study tools, including two complete practice exams, over a hundred electronic flashcards, and more.Rev

Criticality safety analysis of Hanford Waste Tank 241-101-SY

International Nuclear Information System (INIS)

Perry, R.T.; Sapir, J.L.; Krohn, B.J.

1993-01-01

As part of a safety assessment for proposed pump mixing operations to mitigate episodic gas releases in Tank 241-101-SY at the Hanford Site, Richland, Washington, a criticality safety analysis was made using the Sn transport code ONEDANT. The tank contains approximately one million gallons of waste and an estimated 910 G of plutonium. the criticality analysis considers reconfiguration and underestimation of plutonium content. The results indicate that Tank SY-101 does not present a criticality hazard. These methods are also used in criticality analyses of other Hanford tanks

Project Turnover Deliverables for the SY Farm Enraf Annulus Leak Detectors

International Nuclear Information System (INIS)

SCAIEF, C.C.

2000-01-01

This document identifies the deliverables that ensure the end user of the SY Farm Enraf Annulus Leak Detectors (ALD) has all the documentation and training required for operating and maintaining the new system. All deliverable items checked on the Acceptance For Beneficial Use (ABU) form have been completed and are available to the end user. This document was written as required by HNF-IP-0842, Volume IV section 3.12 Acceptance of Structures, Systems, and Components for Beneficial Use. This document applies to the deliverable documentation required to operate and maintain the SY Farm Enraf ALD System. Appendix A provides a copy of the ABU form as listed in the appendix of TWR-4092, Engineering Task Plan for the New SY Farm Annulus Leak Detectors. This document attests that all required deliverable items checked on the ABU have been completed and are available to the end user

The Macquarie Dictionary, its History and its Editorial Practices Die Macquarie Dictionary, sy geskiedenis en sy redaksionele praktyke

Directory of Open Access Journals (Sweden)

Arthur Delbridge

2012-09-01

Full Text Available

The Macquarie Dictionary, first published in Sydney in 1981, was intended to be the first comprehensive dictionary of Australian English. Now in its third edition it has been widely adopted by institutions and the general community as the national dictionary. This paper traces its development from a difficult birth to its present maturity, from a large set of cards to an electronic database, from a single book to a lexicographic library. The rationale and the methodology are laid out along with an account of the reception given to the dictionary in Australia and internationally.

Keywords: dictionary; lexicography; macquarie dictionary; australianness; australianise; australian english; national dictionaries; phonology; international phonetic alphabet; lexical labels; lexicographic style; language style; colloquial; aboriginal words; electronic database; corpus; citation

Die Macquarie Dictionary wat vir die eerste keer in 1981 in Sydney gepubliseer is, was bedoel om die eerste omvattende woordeboek van Australiese Engels te wees. Die woordeboek wat tans in sy derde uitgawe is, word reeds algemeen deur instansies en die algemene publiek as nasionale woordeboek aanvaar. Hierdie artikel skets sy ontwikkeling vanaf 'n moeilike geboorte tot sy huidige volwassenheid, vanaf 'n groot versameling kaartjies tot 'n elektroniese databasis, vanaf 'n enkele boek tot 'n leksikografiese biblioteek. Die grondbeginsels en metodologie word gegee saam met 'n verslag van die ontvangs wat dit in Australië en internasionaal gekry het.

Sleutelwoorde: woordeboek; leksikografie; macquarie dictionary; australiesheid; australianiseer; australiese engels; nasionale woordeboeke; fonologie; internasionale fonetiese alfabet; leksikale etikette; leksikografiese styl; taalstyl; omgangstaal; aboriginele woorde; elektroniese databasis; korpus; aanhaling

SyZyGy: A straight interferometric spacecraft system for gravity wave observations

International Nuclear Information System (INIS)

Estabrook, F.B.; Armstrong, J.W.; Tinto, Massimo; Folkner, William

2003-01-01

We consider a spaceborne gravitational wave (GW) detector formed by three spacecraft in a linear array, coherently exchanging laser beams and using the data combinations of time-delay interferometry (TDI). We previously showed how the measured time series of Doppler shifts in the six one-way laser links between spacecraft pairs in a general unequal-arm triangular configuration can be combined, using TDI, to exactly cancel the otherwise overwhelming phase noise of the lasers while retaining sensitivity to GWs. Here we apply TDI, unfolding the general triangular configuration, to the special case of a linear array of three spacecraft. We show that such an array ('SyZyGy') has, compared with an equilateral triangle GW detector of the same scale, a degraded (but non-zero) sensitivity at low frequencies [f -4 -10 -1 Hz). SyZyGy with ∼1 light-second scale could, for the same instrumental assumptions as LISA, make observations in this intermediate frequency GW band with 5σ sensitivity to sinusoidal waves ≅2.5x10 -23 in a year's integration

Structural analysis and evaluation of the 241SY101 tank annulus heat-up

International Nuclear Information System (INIS)

Ziada, H.H.

1994-01-01

This document provides the structural analysis (static and thermal loads) of the 241SY101 tank to determine the maximum allowable temperature and rate of heating that could be applied to tank 241SY101 through annulus air heating without detrimental effects to the structural integrity of the concrete and steel liner of the tank

Tank 241-SY-101 push mode core sampling and analysis plan

International Nuclear Information System (INIS)

CONNER, J.M.

1998-01-01

This sampling and analysis plan (SAP) identifies characterization objectives pertaining to sample collection, laboratory analytical evaluation, and reporting requirements for push mode core samples from tank 241-SY-101 (SY-101). It is written in accordance with Data Quality Objective to Support Resolution of the Flammable Gas Safety Issue (Bauer 1998), Low Activity Waste Feed Data Quality Objectives (Wiemers and Miller 1997 and DOE 1998), Data Quality Objectives for TWRS Privatization Phase I: Confirm Tank T is an Appropriate Feed Source for Low-Activity Waste Feed Batch X (Certa 1998), and the Tank Safety Screening Data Quality Objective (Dukelow et al. 1995). The Tank Characterization Technical Sampling Basis document (Brown et al. 1998) indicates that these issues apply to tank SY-101 for this sampling event. Brown et al. also identifies high-level waste, regulatory, pretreatment and disposal issues as applicable issues for this tank. However, these issues will not be addressed via this sampling event

Ras-MAPK signaling in differentiating SH-SY5Y human neuroblastoma cells

OpenAIRE

Olsson, Anna-Karin

2000-01-01

Neuroblastoma is a malignant childhood cancer, originating from sympathetic neuroblasts of the peripheral nervous system. Neuroblastoma is a heterogenous group of tumours, while some are highly malignant others can spontaneosly mature into a more benign form or regress. Less than half of the patients survive and this statistics has improved only modestly over the past 20 years. SH-SY5Y is a human neuroblastoma cell line established from a highly malignant tumour. The cells have retained a ca...

iSyTE 2.0: a database for expression-based gene discovery in the eye

Science.gov (United States)

Kakrana, Atul; Yang, Andrian; Anand, Deepti; Djordjevic, Djordje; Ramachandruni, Deepti; Singh, Abhyudai; Huang, Hongzhan

2018-01-01

Abstract Although successful in identifying new cataract-linked genes, the previous version of the database iSyTE (integrated Systems Tool for Eye gene discovery) was based on expression information on just three mouse lens stages and was functionally limited to visualization by only UCSC-Genome Browser tracks. To increase its efficacy, here we provide an enhanced iSyTE version 2.0 (URL: http://research.bioinformatics.udel.edu/iSyTE) based on well-curated, comprehensive genome-level lens expression data as a one-stop portal for the effective visualization and analysis of candidate genes in lens development and disease. iSyTE 2.0 includes all publicly available lens Affymetrix and Illumina microarray datasets representing a broad range of embryonic and postnatal stages from wild-type and specific gene-perturbation mouse mutants with eye defects. Further, we developed a new user-friendly web interface for direct access and cogent visualization of the curated expression data, which supports convenient searches and a range of downstream analyses. The utility of these new iSyTE 2.0 features is illustrated through examples of established genes associated with lens development and pathobiology, which serve as tutorials for its application by the end-user. iSyTE 2.0 will facilitate the prioritization of eye development and disease-linked candidate genes in studies involving transcriptomics or next-generation sequencing data, linkage analysis and GWAS approaches. PMID:29036527

Glutathione transferase-M2-2 secreted from glioblastoma cell protects SH-SY5Y cells from aminochrome neurotoxicity.

Science.gov (United States)

Cuevas, Carlos; Huenchuguala, Sandro; Muñoz, Patricia; Villa, Monica; Paris, Irmgard; Mannervik, Bengt; Segura-Aguilar, Juan

2015-04-01

U373MG cells are able to take up aminochrome that induces glutathione transferase M2-2 (GSTM2) expression in a concentration-dependent manner where 100 µM aminochrome increases GSTM2 expression by 2.1-fold (P protects SH-SY5Y cells incubated with 10 µM aminochrome. The significant protection provided by U373MG-conditioned medium in SH-SY5Y cells incubated with aminochrome was dependent on GSTM2 internalization into SH-SY5Y cells as evidenced by (i) uptake of (14)C-GSTM2 released from U373MG cells into SH-SY5Y cells, a process inhibited by anti-GSTM2 antiserum; (ii) lack of protection of U373MG-conditioned medium in the presence of anti-GSTM2 antiserum on SH-SY5Y cells treated with aminochrome; and (iii) lack of protection of conditioned medium from U373MGsiGST6 that expresses an siRNA directed against GSTM2 on SH-SY5Y cells treated with aminochrome. In conclusion, our results demonstrated that U373MG cells protect SH-SY5Y cells against aminochrome neurotoxicity by releasing GSTM2 into the conditioned medium and subsequent internalization of GSTM2 into SH-SY5Y cells. These results suggest a new mechanism of protection of dopaminergic neurons mediated by astrocytes by releasing GSTM2 into the intersynaptic space and subsequent internalization into dopaminergic neuron in order to protect these cells against aminochrome neurotoxicity.

Tietoa terveydestä ja miesten yleisimmistä syövistä : Miehen katsastuskortti

OpenAIRE

Haverila, Erika; Karppinen, Tiina

2017-01-01

Suomessa todetaan yli 27 000 syöpää vuodessa ja siihen kuolee yli 10 000 ihmistä. Syövät ovat yleistyneet väestössä ja yleistyvät jatkossakin, koska ikääntyneiden määrä lisääntyy. Syövän riskitekijöitä ovat ikä, periytyvyys, ympäristötekijät sekä elintavat. Elintavoista tupak-ointi, epäterveellinen ravitsemus, alkoholin liikakäyttö, liian vähäinen liikunta, ylipaino sekä liian runsas altistuminen auringonvalolle ovat tärkeimpiä riskitekijöitä. Terveyden edi-stämistä voidaan pitää yksilön pros...

Ontsporing van die regstaatsbegrip en sy konsekwensies vir die afrikanervolk

Directory of Open Access Journals (Sweden)

N. G.S. Van der Walt

1966-03-01

Full Text Available Die opvatting van die suiwere regstaat bring sy vraagstukke mee wanneer dit in verband gebring word met die leer van die soewereiniteit in eie kring. Laasgenoemde leer wortel in die geloof dat God die mens roep tot diens aan Hom, en dat die mens vry moet wees om ten opsigte van alle lewensaspekte God na die hoogste mate te kan dien. Hierdie verskeidenheid van aspekte is deur dr. A. Kuyper en sy volgelinge gesistemati- seer tot menslike verbande en lewenskringe, elk met ’n be- paalde sfeer waarin ander kringe nie mag inmeng nie. Elkeen is dan soewerein binne sy eie kring. Met verwysing na die staat is dan gespreek van die staatstaak as synde die hand- hawing van die reg, en omdat die staat in toenemende mate hom veral in die latere tyd ook besig gehou het met bedrywig- hede wat nouliks as van juridiese aard beskou kan word, is daar die onderskeid gemaak tussen die primêre en sekundêre taak van die staat. Die sistematiek van die wetsidee, deur prof. Dooyeweerd ontwikkel, waar die staat sy bestemmingsfunksie in die regsaspek vind, het vanselfsprekend geen plek vir so 'n dualistiese staatstaak nie, en die oplossing is deur sommige daarin gesoek om sulke staatsoptrede te vertolk as hebbende wel betrekking op die regsgemeenskap of die regsverkeer. Staatsondernemings soos paaie, spoorweë, spellingreëling, ens. word dan beskou as noodsaaklik vir die instandhouding van die regsgemeenskap en regsverkeer. Tereg wys A. M. Donner1 daarop dat so ’n vertolking eintlik neerkom op ’n oorspanning van die regsbegrip. Hy self sien dan die staatstaak in vrede- of orde-handhawing, wat in die eerste plaas wel regshandha- wing veronderstel. Onder orde word dan alles verstaan wat noodsaaklik is vir die gladde verkeer in die ontwikkelingsgang van die regsgemeenskap. Die wese van die staat het dan vol- gens hom te doen met reg, maar sy taak is meer as suiwere regshandhawing. Wat onrus verwek en die orde versteur, moet uit die openbare lewe verwyder word, en die

101-SY waste sample speed of sound/rheology testing for sonic probe program

International Nuclear Information System (INIS)

Cannon, N.S.

1994-01-01

One problem faced in the clean-up operation at Hanford is that a number of radioactive waste storage tanks are experiencing a periodic buildup and release of potentially explosive gases. The best known example is Tank 241-SY-101 (commonly referred to as 101-SY) in which hydrogen gas periodically built up within the waste to the point that increased buoyancy caused a roll-over event, in which the gas was suddenly released in potentially explosive concentrations (if an ignition source were present). The sonic probe concept is to generate acoustic vibrations in the 101-SY tank waste at nominally 100 Hz, with sufficient amplitude to cause the controlled release of hydrogen bubbles trapped in the waste. The sonic probe may provide a potentially cost-effective alternative to large mixer pumps now used for hydrogen mitigation purposes. Two important parameters needed to determine sonic probe effectiveness and design are the speed of sound and yield stress of the tank waste. Tests to determine these parameters in a 240 ml sample of 101-SY waste (obtained near the tank bottom) were performed, and the results are reported

Autophagy regulates chlorpyrifos-induced apoptosis in SH-SY5Y cells

International Nuclear Information System (INIS)

Park, Jae Hyeon; Lee, Jeong Eun; Shin, In Chul; Koh, Hyun Chul

2013-01-01

Recent studies have shown that up-regulation of autophagy may be a tractable therapeutic intervention for clearing disease-causing proteins, including α-synuclein, ubiquitin, and other misfolded or aggregated proteins in pesticide-induced neurodegeneration. In a previous study, we reported that chlorpyrifos (CPF)-induced mitochondria-dependent apoptosis is mediated through reactive oxygen species in SH-SY5Y cells. In this study, we explored a novel pharmacotherapeutic approach to prevent CPF neurotoxicity involving the regulation of autophagy. We investigated the modulation of CPF-induced apoptosis according to autophagy regulation. We found that CPF induced apoptosis in SH-SY5Y cells, as demonstrated by the activation of caspase-3 and nuclear condensation. In addition, we observed that cells treated with CPF underwent autophagic cell death by monitoring the expression of LC3-II and p62. Pretreatment with the autophagy inducer rapamycin significantly enhanced the cell viability of CPF-exposed cells, and the enhancement of cell viability was partially due to alleviation of CPF-induced apoptosis via a decrease in levels of cleaved caspase-3. Specifically, rapamycin pretreatment decreased Bax and increased Bcl-2 expression in mitochondria. In addition, rapamycin significantly decreased cytochrome c release in from mitochondria into the cytosol. However, pretreatment of cells with the autophagy inhibitor, 3-methyladenine (3MA), remarkably increased CPF toxicity in these cells; this with correlated with increased expression of Bax and decreased expression of Bcl-2 in mitochondria. Our results suggest that CPF-induced cytotoxicity is modified by autophagy regulation and that rapamycin protects against CPF-induced apoptosis by enhancing autophagy. Pharmacologic induction of autophagy by rapamycin may be a useful treatment strategy in neurodegenerative disorders. - Highlights: ► Chlorpyrifos (CPF) is cytotoxic to SH-SY5Y cells ► CPF-induced cytotoxicity is mediated by

Autophagy regulates chlorpyrifos-induced apoptosis in SH-SY5Y cells

Energy Technology Data Exchange (ETDEWEB)

Park, Jae Hyeon [Department of Pharmacology, College of Medicine, Hanyang University (Korea, Republic of); Hanyang Biomedical Research Institute, Seoul (Korea, Republic of); Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul (Korea, Republic of); Lee, Jeong Eun [Department of Pharmacology, College of Medicine, Hanyang University (Korea, Republic of); Hanyang Biomedical Research Institute, Seoul (Korea, Republic of); Shin, In Chul [Department of Pharmacology, College of Medicine, Hanyang University (Korea, Republic of); Koh, Hyun Chul, E-mail: hckoh@hanyang.ac.kr [Department of Pharmacology, College of Medicine, Hanyang University (Korea, Republic of); Hanyang Biomedical Research Institute, Seoul (Korea, Republic of); Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul (Korea, Republic of)

2013-04-01

Recent studies have shown that up-regulation of autophagy may be a tractable therapeutic intervention for clearing disease-causing proteins, including α-synuclein, ubiquitin, and other misfolded or aggregated proteins in pesticide-induced neurodegeneration. In a previous study, we reported that chlorpyrifos (CPF)-induced mitochondria-dependent apoptosis is mediated through reactive oxygen species in SH-SY5Y cells. In this study, we explored a novel pharmacotherapeutic approach to prevent CPF neurotoxicity involving the regulation of autophagy. We investigated the modulation of CPF-induced apoptosis according to autophagy regulation. We found that CPF induced apoptosis in SH-SY5Y cells, as demonstrated by the activation of caspase-3 and nuclear condensation. In addition, we observed that cells treated with CPF underwent autophagic cell death by monitoring the expression of LC3-II and p62. Pretreatment with the autophagy inducer rapamycin significantly enhanced the cell viability of CPF-exposed cells, and the enhancement of cell viability was partially due to alleviation of CPF-induced apoptosis via a decrease in levels of cleaved caspase-3. Specifically, rapamycin pretreatment decreased Bax and increased Bcl-2 expression in mitochondria. In addition, rapamycin significantly decreased cytochrome c release in from mitochondria into the cytosol. However, pretreatment of cells with the autophagy inhibitor, 3-methyladenine (3MA), remarkably increased CPF toxicity in these cells; this with correlated with increased expression of Bax and decreased expression of Bcl-2 in mitochondria. Our results suggest that CPF-induced cytotoxicity is modified by autophagy regulation and that rapamycin protects against CPF-induced apoptosis by enhancing autophagy. Pharmacologic induction of autophagy by rapamycin may be a useful treatment strategy in neurodegenerative disorders. - Highlights: ► Chlorpyrifos (CPF) is cytotoxic to SH-SY5Y cells ► CPF-induced cytotoxicity is mediated by

CASE STUDIES OF RADON REDUCTION RESEARCH IN MARYLAND, NEW JERSEY, AND VIRGINIA SCHOOLS

Science.gov (United States)

The report gives results of radon mitigation research conducted in 1991 and 1992 in school buildings in Maryland, New Jersey, and Virginia. One school in each state was selected. In two schools, the objective was to evaluate the potential for modifying the school ventilation sy...

An assessment of the dilution required to mitigate Hanford tank 241-SY-101

International Nuclear Information System (INIS)

Hudson, J.D.; Bredt, P.R.; Felmy, A.R.; Stewart, C.W.; Tingey, J.M.; Trent, D.S.; Barney, G.S.; Herting, D.L.; Larrick, A.P.; Reynolds, D.A.

1995-02-01

A group of experts from PNL and WHC convened November 2 and 3, 1994, to screen the current state of knowledge about dilution and reach a consensus on the minimum dilution ratio that will achieve passive mitigation of Tank 241-SY-101 wastes and the dilution ratio that would satisfy the given cross-site transfer criteria with reasonable assurance. The panel evaluated the effects of dilution on the parameters important in gas generation, retention, and release and reached the following conclusions, which are deduced from the existing body of data, experience, and analyses: (1) Dissolution of solids is the single most important aspect of mitigation by dilution. We are confident that diluting until nitrates, nitrites, and aluminum salts are dissolved will mitigate Hanford flammable gas tanks; (2) Sufficient solids dissolution can be achieved in Tank 241-SY-101 at a dilution ratio of 1:1, which will result in a average specific gravity of approximately 1.35. It is likely that a 0.5:1 dilution will also mitigate 241-SY-101, but the current uncertainty is too high to recommend this dilution ratio; (3) The recommended dilution requires a diluent with at least 2 molar free hydroxide, because aluminum probably precipitates at lower hydroxide concentrations. The transfer criteria for Tank 241-SY-101 waste were also evaluated. These criteria have been specified as solids content ≤30% (volume), viscosity ≤30% cP and density <1.5 g/mL. (1) Solids content is the limiting criterion if it is defined as volume fraction of settled solids. A 1:1 dilution will satisfy this criterion at nominal premixing conditions in Tank 241-SY-101; however, analysis of Window E core samples suggests that up to 1.5:1 might be required. If the solids content is interpreted simply as solids volume fraction no further dilution is necessary, because Tank 241-SY-101 waste (excluding the crust) is already below 30%; (2) Bulk density is the next limiting criterion and is met at 0.4:1 dilution

Syed Manzoorul Islam's Postmodern Tales: A Study

Science.gov (United States)

Sarker, Md Abdul Momen; Talukder, Tusar

2016-01-01

The paper brings into focus how Syed Manzoorul Islam, in his three-decade-long literary career, has mastered a narrative style that sets him apart from many of his Bengali contemporaries. It demonstrates all the traits unique to his storytelling: blurring of boundaries between dream and reality, self-reflexivity, irony, and humor. The research…

241-SY-101 data acquisition and control system (DACS) remote operator interface operational test report

International Nuclear Information System (INIS)

ERMI, A.M.

1999-01-01

The readiness of the upgraded 241-SY-101 Data Acquisition and Control System (DACS) to provide proper control and monitoring of the mixer pump and instrumentation in tank 241-SY-101 was evaluated by the performance of OTP-440-001. Results of the OTP are reported here

Tank 241-SY-103 tank characterization plan. Revision 1

International Nuclear Information System (INIS)

Homi, C.S.

1995-01-01

This document is a plan that identifies the information needed to address relevant issues concerning short-term and long-term safe storage and long-term management of Single-Shell Tank (SST) 241-SY-103

Paracrine Maturation and Migration of SH-SY5Y Cells by Dental Pulp Stem Cells.

Science.gov (United States)

Gervois, P; Wolfs, E; Dillen, Y; Hilkens, P; Ratajczak, J; Driesen, R B; Vangansewinkel, T; Bronckaers, A; Brône, B; Struys, T; Lambrichts, I

2017-06-01

Neurological disorders are characterized by neurodegeneration and/or loss of neuronal function, which cannot be adequately repaired by the host. Therefore, there is need for novel treatment options such as cell-based therapies that aim to salvage or reconstitute the lost tissue or that stimulate host repair. The present study aimed to evaluate the paracrine effects of human dental pulp stem cells (hDPSCs) on the migration and neural maturation of human SH-SY5Y neuroblastoma cells. The hDPSC secretome had a significant chemoattractive effect on SH-SY5Y cells as shown by a transwell assay. To evaluate neural maturation, SH-SY5Y cells were first induced toward neuronal cells, after which they were exposed to the hDPSC secretome. In addition, SH-SY5Y cells subjected to the hDPSC secretome showed increased neuritogenesis compared with nonexposed cells. Maturated cells were shown to increase immune reactivity for neuronal markers compared with controls. Ultrastructurally, retinoic acid (RA) signaling and subsequent exposure to the hDPSC secretome induced a gradual rise in metabolic activity and neuronal features such as multivesicular bodies and cytoskeletal elements associated with cellular communication. In addition, electrophysiological recordings of differentiating cells demonstrated a transition toward a neuronal electrophysiological profile based on the maximum tetrodotoxin (TTX)-sensitive, Na + current. Moreover, conditioned medium (CM)-hDPSC-maturated SH-SY5Y cells developed distinct features including, Cd 2+ -sensitive currents, which suggests that CM-hDPSC-maturated SH-SY5Y acquired voltage-gated Ca 2+ channels. The results reported in this study demonstrate the potential of hDPSCs to support differentiation and recruitment of cells with neuronal precursor characteristics in a paracrine manner. Moreover, this in vitro experimental design showed that the widely used SH-SY5Y cell line can improve and simplify the preclinical in vitro research on the molecular

Work plan for SY Farm Integrated Data Acquisition and Control System (DACS-2a)

International Nuclear Information System (INIS)

Conner, R.P.; Katz, R.S.

1994-01-01

The SY Farm currently has a temporary Data Acquisition ampersand Control System (DACS) housed in a mobile trailer. The system is currently referred to as DACS-1. It was designed and configured to support engineers and scientists conducting the special performance evaluation and testing program for the safety mitigation test equipment located in waste tank 241-SY-101 (101-SY). It is currently being maintained and utilized by engineering personnel to monitor and control the 101-SY mitigation pump activities. Based upon the results of the mitigation testing program, some of the temporary test mitigation equipment (such as mixing pump) will be replaced with longer-term ''operational'' mitigation equipment. This is resulting in new requirements for the Data Acquisition and Control System which will be full-filled by a newer control facility referred to as the DACS-2. A teaming between Westinghouse Hanford Company (WHC) and Los Alamos National Laboratory (LANL) has been established for the SY farm mitigation program in order to develop and implement the ''next generation'' of the data acquisition and control system for the mitigation pump operations. The new system will be configured for use by the tank farm operational personnel. It will support the routine operations necessary for safety mitigation and the future waste retrieval of Project W-211. It is intended to replace the existing DACS-1 and provide the necessary control room space for future integration of W-211

Level maintenance for Tank 101-SY mitigation-by-mixing test. Revision 2

International Nuclear Information System (INIS)

Larsen, D.C.

1994-01-01

The Phase A, Phase B and Full Scale testing portions of the Mitigation-By-Mixing Test have demonstrated the effectiveness of the Mixer Pump to maintain the waste in tank 101-SY in the desired mitigated state. The operation of the 101-SY Mixer Pump for short periods of time results in a controlled release of hydrogen gas in concentrations well below the established safety limits. Additionally, it has been shown that operation of the pump on a regular schedule minimizes the historical generation rate of hydrogen inventory in the waste. Generation of hydrogen inventory is exhibited by waste level growth. The primary objective of this procedure is to maintain the waste level in tank 241-SY-101 within the safe operating range as defined by the Safety Assessment and the Test Plan. The secondary objective is to operate the pump on a schedule that maximizes its useful lifespan and prevents the formation of obstructions in the normal flow path of the pump

A survey of available information on gas generation in tank 241-SY-101: Hanford Tank Safety Project

International Nuclear Information System (INIS)

Strachan, D.M.; Reynolds, D.A.; Siemer, D.D.; Wallace, R.W.

1991-03-01

As a result of a concerted effort to determine the chemical and physical mechanisms underlying the generation and episodic release of gases from tank 241-SY-101, more commonly known as tank 101-SY, the Tank Waste Science Panel has been established at the Pacific Northwest Laboratory. Four of the members of this panel met to screen the available information on tank 101-SY and provide to the remaining members a shortened list of references that could be used to assess the mechanisms underlying the generation and episodic release of gases from tank 101-SY. This document is the result of this preliminary screening of information for the Tank Waste Science Panel and was provided to the Panel members at their first meeting. 14 refs., 3 tabs

Hysterosalpingo-foam sonography (HyFoSy): Tolerability, safety and the occurrence of pregnancy post-procedure.

Science.gov (United States)

Tanaka, Keisuke; Chua, Jackie; Cincotta, Robert; Ballard, Emma L; Duncombe, Gregory

2018-02-01

Fallopian tube patency testing is an essential part of infertility evaluation. Hysterosalpingo-contrast sonography (HyCoSy) has been described as reliable, well tolerated and safe compared to other modalities such as laparoscopy and a dye test or hysterosalpingography. Limited availability of the previously used contrast has led to the introduction of a foam contrast agent as an alternative. To assess the tolerability, safety and occurrence of pregnancy post-procedure of hysterosalpingo-foam sonography (HyFoSy). A retrospective cohort study of women who had a HyFoSy at Queensland Ultrasound for Women from March 2013 to February 2015. A questionnaire was sent to their referring doctor to identify any complications or subsequent pregnancies with or without artificial reproductive technology (ART) within six months of the HyFoSy. Of 200 women, four cases were abandoned due to difficulty introducing the intracervical catheter, severe discomfort or a vasovagal episode. Response from referring doctors for 155 women reported no post-procedural complication. One hundred and eleven women were followed up for at least six months. Twenty-four out of 59 women (40.7%) who had ART and 24 out of 52 women (46.2%) who did not have ART conceived. Fifty percent of women who were nulligravida at the time of investigation, found to have at least one patent fallopian tube, whose partner had a normal semen analysis, spontaneously conceived within the time of follow up. HyFoSy is well tolerated and safe. A preponderance of pregnancies in the first month after HyFoSy suggests that a therapeutic effect may exist. © 2017 The Royal Australian and New Zealand College of Obstetricians and Gynaecologists.

Operability test procedure [Tank] 241-SY-101 equipment removal system

International Nuclear Information System (INIS)

Mast, J.C.

1994-01-01

The 241-SY-101 equipment removal system (ERS) consists of components, equipment, instrumentation and procedures that will provide the means to disconnect, retrieve, contain, load and transport the Mitigation Pump Assembly (MPA) from waste Tank 241-SY-101 to the Central Waste Complex (CWC). The Operability Test Procedure (OTP) will test the interfaces between ERS components and will rehearse the procedure for MPA removal and transportation to the extent they can be mocked-up at the CTF (Cold Test Facility). At the conclusion of the OTP, the ERS components and equipment will be removed from the CTF, entered into the Component Based Recall System (CBRS), and stored until needed for actual MPA removal and transportation

A Discussion of SY-101 Crust Gas Retention and Release Mechanisms

International Nuclear Information System (INIS)

Mendoza, D.P.; Mahoney, L.A.; Gauglitz, P.A.; Rassat, S.D.; Caley, S.M.

1999-01-01

The flammable gas hazard in Hanford waste tanks was made an issue by the behavior of double-shell Tank (DST) 241-SY-101 (SY-101). Shortly after SY-101 was filled in 1980, the waste level began rising periodically, due to the generation and retention of gases within the slurry, and then suddenly dropping as the gases were released. An intensive study of the tank's behavior revealed that these episodic releases posed a safety hazard because the released gas was flammable, and, in some cases, the volume of gas released was sufficient to exceed the lower flammability limit (LFL) in the tank headspace (Alleinann et al. 1993). A mixer pump was installed in SY-101 in late 1993 to prevent gases from building up in the settled solids layer, and the large episodic gas releases have since ceased (Allemann et al. 1994; Stewart et al. 1994; Brewster et al. 1995). However, the surface level of SY-101 has been increasing since at least 1995, and in recent months the level growth has shown significant and unexpected acceleration. Based on a number of observations and measurements, including data from the void fraction instrument (VFI), we have concluded that the level growth is caused largely by increased gas retention in the floating crust. In September 1998, the crust contained between about 21 and 43% void based on VFI measurements (Stewart et al. 1998). Accordingly, it is important to understand the dominant mechanisms of gas retention, why the gas retention is increasing, and whether the accelerating level increase will continue, diminish or even reverse. It is expected that the retained gas in the crust is flammable, with hydrogen as a major constituent. This gas inventory would pose a flammable gas hazard if it were to release suddenly. In May 1997, the mechanisms of bubble retention and release from crust material were the subject of a workshop. The evaluation of the crust and potential hazards assumed a more typical void of roughly 15% gas. It could be similar to

A Discussion of SY-101 Crust Gas Retention and Release Mechanisms

Energy Technology Data Exchange (ETDEWEB)

SD Rassat; PA Gauglitz; SM Caley; LA Mahoney; DP Mendoza

1999-02-23

The flammable gas hazard in Hanford waste tanks was made an issue by the behavior of double-shell Tank (DST) 241-SY-101 (SY-101). Shortly after SY-101 was filled in 1980, the waste level began rising periodically, due to the generation and retention of gases within the slurry, and then suddenly dropping as the gases were released. An intensive study of the tank's behavior revealed that these episodic releases posed a safety hazard because the released gas was flammable, and, in some cases, the volume of gas released was sufficient to exceed the lower flammability limit (LFL) in the tank headspace (Allemann et al. 1993). A mixer pump was installed in SY-101 in late 1993 to prevent gases from building up in the settled solids layer, and the large episodic gas releases have since ceased (Allemann et al. 1994; Stewart et al. 1994; Brewster et al. 1995). However, the surface level of SY-101 has been increasing since at least 1995, and in recent months the level growth has shown significant and unexpected acceleration. Based on a number of observations and measurements, including data from the void fraction instrument (VFI), we have concluded that the level growth is caused largely by increased gas retention in the floating crust. In September 1998, the crust contained between about 21 and 43% void based on VFI measurements (Stewart et al. 1998). Accordingly, it is important to understand the dominant mechanisms of gas retention, why the gas retention is increasing, and whether the accelerating level increase will continue, diminish or even reverse. It is expected that the retained gas in the crust is flammable, with hydrogen as a major constituent. This gas inventory would pose a flammable gas hazard if it were to release suddenly. In May 1997, the mechanisms of bubble retention and release from crust material were the subject of a workshop. The evaluation of the crust and potential hazards assumed a more typical void of roughly 15% gas. It could be similar to

Design analysis supporting 101-SY Water Decon System

International Nuclear Information System (INIS)

Cleveland, K.J.

1995-01-01

This document contains the results of stress analysis and component sizing for the 101-SY mitigation pump, Water Decon System. Calculations included are a stress analysis of the High Pressure Manifold, the threaded connection on the Yoke Water Connector and a sizing of an air receiver tank

241-SY-101 multi-functional instrument tree acceptance for beneficial use (ABU)

International Nuclear Information System (INIS)

Erhart, M.F.

1995-01-01

This document formally demonstrates that the ABU process for the 241-SY-101 risers 17B and 17C Multi-functional Instrument Trees (MIT's) has been properly completed in accordance with the approved ABU checklists. For each item required on the ABU Checklist, a bibliography of the documentation prepared and released to satisfy the requirements is provided. Release of this documentation signifies that the tank farm Operations, Engineering, and Maintenance organizations have accepted responsibility for the MIT'S in 241-SY-101 Risers 17B and 17C

SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson's disease.

Science.gov (United States)

Xie, Hong-rong; Hu, Lin-sen; Li, Guo-yi

2010-04-20

To evaluate the human neuroblastoma SH-SY5Y cell line as an in vitro model of dopaminergic (DAergic) neurons for Parkinson's disease (PD) research and to determine the effect of differentiation on this cell model. The data of this review were selected from the original reports and reviews related to SH-SY5Y cells published in Chinese and foreign journals (Pubmed 1973 to 2009). After searching the literature, 60 articles were selected to address this review. The SH-SY5Y cell line has become a popular cell model for PD research because this cell line posses many characteristics of DAergic neurons. For example, these cells express tyrosine hydroxylase and dopamine-beta-hydroxylase, as well as the dopamine transporter. Moreover, this cell line can be differentiated into a functionally mature neuronal phenotype in the presence of various agents. Upon differentiation, SH-SY5Y cells stop proliferating and a constant cell number is subsequently maintained. However, different differentiating agents induce different neuronal phenotypes and biochemical changes. For example, retinoic acid induces differentiation toward a cholinergic neuronal phenotype and increases the susceptibility of SH-SY5Y cells to neurotoxins and neuroprotective agents, whereas treatment with retinoic acid followed by phorbol ester 12-O-tetradecanoylphorbol-13-acetate results in a DAergic neuronal phenotype and decreases the susceptibility of cells to neurotoxins and neuroprotective agents. Some differentiating agents also alter kinetics of 1-methyl-4-phenyl-pyridinium (MPP(+)) uptake, making SH-SY5Y cells more similar to primary mesencephalic neurons. Differentiated and undifferentiated SH-SY5Y cells have been widely used as a cell model of DAergic neurons for PD research. Some differentiating agents afford SH-SY5Y cells with more potential for studying neurotoxicity and neuroprotection and are thus more relevant to experimental PD research.

The 1984 eclipse of the symbiotic binary SY Muscae

Science.gov (United States)

Kenyon, S. J.; Michalitisianos, A. G.; Lutz, J. H.; Kafatos, M.

1985-01-01

Data from IUE spectra obtained with the 10 x 20-arcsec aperture on May 13, 1984, and optical spectrophotometry obtained with an SIT vidicon on the 1.5-m telescope at CTIO on April 29-May 1, 1984, are reported for the symbiotic binary SY Mus. The data are found to be consistent with a model of a red-giant secondary of 60 solar radii which completely eclipses the hot primary every 627 d but only partially eclipses the 75-solar-radius He(+) region surrounding the primary. The distance to SY Mus is estimated as 1.3 kpc. It is suggested that the large Balmer decrement in eclipse, with (H-alpha)/(H-beta) = 8.3 and (H-beta)/(H-gamma) = 1.5, is associated with an electron density of about 10 to the 10th/cu cm.

241-SY-101 data acquisition and control system (DACS) operator interface upgrade operational test report

International Nuclear Information System (INIS)

ERMI, A.M.

1999-01-01

This procedure provides instructions for readiness of the first portion of the upgraded 241-SY-101 Data Acquisition and Control System (DACS) computer system to provide proper control and monitoring of the mitigation mixer pump and instrumentation installed in the 241-SY-101 underground storage tank will be systematically evaluated by the performance of this procedure

PSA results for Hanford high level waste Tank 101-SY

Energy Technology Data Exchange (ETDEWEB)

MacFarlane, D.R.; Bott, T.F.; Brown, L.F.; Stack, D.W. [Los Alamos National Lab., NM (United States); Kindinger, J.; Deremer, R.K.; Medhekar, S.R.; Mikschl, T.J. [PLG, Inc., Newport Beach, CA (United States)

1993-10-01

Los Alamos National Laboratory has performed a comprehensive probabilistic safety assessment (PSA) that includes consideration of external events for the weapons-production wastes stored in tank number 241-SY-101, commonly known as Tank 101-SY, as configured in December 1992. This tank, which periodically releases (``burps``) a gaseous mixture of hydrogen, nitrous oxide, ammonia, and nitrogen, was analyzed because of public safety concerns associated with the potential for release of radioactive tank contents should this gas mixture be ignited during one of the burps. In an effort to mitigate the burping phenomenon, an experiment is underway in which a large pump has been inserted into the tank to determine if pump-induced circulation of the tank contents will promote a slow, controlled release of the gases. This PSA for Tank 101-SY, which did not consider the pump experiment or future tank-remediation activities, involved three distinct tasks. First, the accident sequence analysis identified and quantified those potential accidents whose consequences result in tank material release. Second, characteristics and release paths for the airborne and liquid radioactive source terms were determined. Finally, the consequences, primarily onsite and offsite potential health effects resulting from radionuclide release, were estimated, and overall risk curves were constructed. An overview of each of these tasks and a summary of the overall results of the analysis are presented in the following sections.

PSA results for Hanford high level waste Tank 101-SY

International Nuclear Information System (INIS)

MacFarlane, D.R.; Bott, T.F.; Brown, L.F.; Stack, D.W.; Kindinger, J.; Deremer, R.K.; Medhekar, S.R.; Mikschl, T.J.

1993-01-01

Los Alamos National Laboratory has performed a comprehensive probabilistic safety assessment (PSA) that includes consideration of external events for the weapons-production wastes stored in tank number 241-SY-101, commonly known as Tank 101-SY, as configured in December 1992. This tank, which periodically releases (''burps'') a gaseous mixture of hydrogen, nitrous oxide, ammonia, and nitrogen, was analyzed because of public safety concerns associated with the potential for release of radioactive tank contents should this gas mixture be ignited during one of the burps. In an effort to mitigate the burping phenomenon, an experiment is underway in which a large pump has been inserted into the tank to determine if pump-induced circulation of the tank contents will promote a slow, controlled release of the gases. This PSA for Tank 101-SY, which did not consider the pump experiment or future tank-remediation activities, involved three distinct tasks. First, the accident sequence analysis identified and quantified those potential accidents whose consequences result in tank material release. Second, characteristics and release paths for the airborne and liquid radioactive source terms were determined. Finally, the consequences, primarily onsite and offsite potential health effects resulting from radionuclide release, were estimated, and overall risk curves were constructed. An overview of each of these tasks and a summary of the overall results of the analysis are presented in the following sections

Seymour (Sy) Epstein (1924-2016).

Science.gov (United States)

O'Brien, Edward; O'Brien, Jean; Waters, Everett

2018-01-01

Presents an obituary for Seymour (Sy) Epstein, who passed away in Amherst on May 20, 2016. Epstein was a significant voice in personality psychology for more than six decades. His wide-ranging, programmatic research addressed cognitive mediation of classical conditioning, stress and coping, the self-concept, drive, conflict, and psychopathology. His research on aggregation strongly influenced the person-situation debate. His most important contribution was his decades-long development of an integrated personality theory, culminating in his magnum opus, Cognitive-Experiential Theory (Oxford University Press, 2014). (PsycINFO Database Record (c) 2018 APA, all rights reserved).

sY116, a human Y-linked polymorphic STS

Indian Academy of Sciences (India)

linked STS, showed different electrophoretic mobilities in three males, two infertile and one fertile. A study of this STS among 35 other normal males showed that this locus is polymorphic. sY116 has a poly A-rich stretch whose instability ...

Compressed air piping, 241-SY-101 hydraulic pump retrieval trailer

International Nuclear Information System (INIS)

Wilson, T.R.

1994-01-01

The following Design Analysis was prepared by the Westinghouse Hanford Company to determine pressure losses in the compressed air piping installed on the hydraulic trailer for the 241-SY-101 pump retrieval mission

Morphological Differentiation Towards Neuronal Phenotype of SH-SY5Y Neuroblastoma Cells by Estradiol, Retinoic Acid and Cholesterol

OpenAIRE

Teppola, Heidi; Sarkanen, Jertta-Riina; Jalonen, Tuula O.; Linne, Marja-Leena

2015-01-01

Human SH-SY5Y neuroblastoma cells maintain their potential for differentiation and regression in culture conditions. The induction of differentiation could serve as a strategy to inhibit cell proliferation and tumor growth. Previous studies have shown that differentiation of SH-SY5Y cells can be induced by all-trans-retinoic-acid (RA) and cholesterol (CHOL). However, signaling pathways that lead to terminal differentiation of SH-SY5Y cells are still largely unknown. The goal of this study was...

Morphological Differentiation Towards Neuronal Phenotype of SH-SY5Y Neuroblastoma Cells by Estradiol, Retinoic Acid and Cholesterol

OpenAIRE

Teppola, Heidi; Sarkanen, Jertta-Riina; Jalonen, Tuula; Linne, Marja-Leena

2016-01-01

Human SH-SY5Y neuroblastoma cells maintain their potential for differentiation and regression in culture conditions. The induction of differentiation could serve as a strategy to inhibit cell proliferation and tumor growth. Previous studies have shown that differentiation of SH-SY5Y cells can be induced by all-trans-retinoic-acid (RA) and cholesterol (CHOL). However, signaling pathways that lead to terminal differentiation of SH-SY5Y cells are still largely unknown. The goal of this study was...

Hydrogen Peroxide Toxicity Induces Ras Signaling in Human Neuroblastoma SH-SY5Y Cultured Cells

Directory of Open Access Journals (Sweden)

Jirapa Chetsawang

2010-01-01

Full Text Available It has been reported that overproduction of reactive oxygen species occurs after brain injury and mediates neuronal cells degeneration. In the present study, we examined the role of Ras signaling on hydrogen peroxide-induced neuronal cells degeneration in dopaminergic neuroblastoma SH-SY5Y cells. Hydrogen peroxide significantly reduced cell viability in SH-SY5Y cultured cells. An inhibitor of the enzyme that catalyzes the farnesylation of Ras proteins, FTI-277, and a competitive inhibitor of GTP-binding proteins, GDP-beta-S significantly decreased hydrogen peroxide-induced reduction in cell viability in SH-SY5Y cultured cells. The results of this study might indicate that a Ras-dependent signaling pathway plays a role in hydrogen peroxide-induced toxicity in neuronal cells.

The Systemic Sclerosis Questionnaire (SySQ): Validation of the translation of the original German version into Spanish and its relationship to the disease and to quality of life.

Science.gov (United States)

Cruz-Domínguez, Maria Pilar; Casarrubias-Ramírez, Moisés; Gasca-Martínez, Victor; Maldonado García, Cindy; Carranza-Muleiro, Rosa Angélica; Medina, Gabriela; García-Collinot, Grettel; Montes-Cortes, Daniel Hector

2017-12-11

Translation, transculturation and validity of the self-administered questionnaire for functionality (Systemic Sclerosis Questionnaires [SySQ]) for use in Spanish patients with systemic sclerosis and its relationship to the severity of the disease and to quality of life. We conducted an observational analytical study to perform a cross-cultural validation of the self-administered questionnaire on functionality in scleroderma. The validity of the form and content was evaluated by an expert panel. The method included: a) adaptation into Spanish of the construct for translation and back translation, and transculturation; b) internal consistency with the SySQ (Cronbach's alpha), and c) reproducibility was assessed taking into account all occasions in which the test was performed with Cohen's kappa. Additionally, we calculated the Spearman correlation coefficient with the Medsger severity scale, Health Assessment Questionnaire score and SF-36 score. We included 70 patients with systemic sclerosis: age 17-78 (51±12) years, 65 (93%) were women, diffuse/limited subtype 64/36%, disease duration of 0.5-40 years. Optimal internal consistency for all categories of the final version of SySQ (Cronbach's α of 0.961) and intraobserver reliability in 2 tests over a 2-week interval (Cohen's kappa coefficient 0.618) and optimal interobserver reliability in 2 tests on the same day (Cohen's kappa coefficient 0.911). Moderate correlation between functionality by SySQ and by Health Assessment Questionnaire (r=0.573, P<.0001). Inverse correlation between SySQ and quality of life mental health domain SF-36 (r=-0.435, P<.001) and physical domain SF-36 (r=-0.638, P<.001). Medsger severity scale (tendon, heart, lung, vascular) also showed significant correlation with SySQ. SySQ in this validated Spanish version is a suitable instrument to measure functional status in patients with systemic sclerosis. Reduced functionality is related to greater tendon and peripheral vascular involvement and

Dichloroacetate stimulates changes in the mitochondrial network morphology via partial mitophagy in human SH-SY5Y neuroblastoma cells

Czech Academy of Sciences Publication Activity Database

Pajuelo-Reguera, David; Alán, Lukáš; Olejár, Tomáš; Ježek, Petr

2015-01-01

Roč. 46, č. 6 (2015), s. 2409-2418 ISSN 1019-6439 R&D Projects: GA MŠk(CZ) EE2.3.30.0025 Institutional support: RVO:67985823 Keywords : dichloroacetate * mitochondria * mitophagy * neuroblastoma SH-SY5Y cells * mitochondrial network Subject RIV: EI - Biotechnology ; Bionics Impact factor: 3.018, year: 2015

SenSyF Experience on Integration of EO Services in a Generic, Cloud-Based EO Exploitation Platform

Science.gov (United States)

Almeida, Nuno; Catarino, Nuno; Gutierrez, Antonio; Grosso, Nuno; Andrade, Joao; Caumont, Herve; Goncalves, Pedro; Villa, Guillermo; Mangin, Antoine; Serra, Romain; Johnsen, Harald; Grydeland, Tom; Emsley, Stephen; Jauch, Eduardo; Moreno, Jose; Ruiz, Antonio

2016-08-01

SenSyF is a cloud-based data processing framework for EO- based services. It has been pioneer in addressing Big Data issues from the Earth Observation point of view, and is a precursor of several of the technologies and methodologies that will be deployed in ESA's Thematic Exploitation Platforms and other related systems.The SenSyF system focuses on developing fully automated data management, together with access to a processing and exploitation framework, including Earth Observation specific tools. SenSyF is both a development and validation platform for data intensive applications using Earth Observation data. With SenSyF, scientific, institutional or commercial institutions developing EO- based applications and services can take advantage of distributed computational and storage resources, tailored for applications dependent on big Earth Observation data, and without resorting to deep infrastructure and technological investments.This paper describes the integration process and the experience gathered from different EO Service providers during the project.

Miscellaneous component design for Tank 241SY101 pump removal

International Nuclear Information System (INIS)

Huang, F.H.

1995-01-01

A mixer pump has been used to mitigate the hydrogen build-up in tank 241SY101 (SY101), located in the 200 West Area of the Hanford Site. New equipment is being prepared for the removal, transport, storage, and disposal of the test pump. The disposal equipment for the test pump now in tank SY101 includes a shipping container, a strong back, a lifting beam, a test weight, container support stands, a modified mock-up pump, a flexible receiver blast shield, a lifting yoke, and a yoke brace. The structural evaluations of container and strong back are detailed in another supporting document (WHC 1994a), the engineering analyses of flexible receiver blast shield/lifting yoke and yoke brace are given in other supporting documents (WHC 1994b, WHC 1994c), respectively. Engineering tasks that were contracted to Advanced Engineering Consultants (AEC) include the design and analysis of the following. Two spreader-beam lifting devices. a Container test weight. Container support saddles. Mock-up pump modification. This report documents the work description, design basis, assumptions, and design calculations provided by AEC for the above components. All AEC documents appear in Appendix A. Additional work conducted by Westinghouse Hanford Company (WHC) on the modified container test weight, modification to the mock-up pump, the removable support for the transport assembly, and saddle modification for air pallets also are included in this document

Tank characterization report for double-shell tank 241-SY-103

International Nuclear Information System (INIS)

Conner, J.M.

1996-01-01

This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in tank 241-SY-103. This report supports the requirements of Tri-Party Agreement Milestone M-44 09

Tank characterization report for double-shell tank 241-SY-103

International Nuclear Information System (INIS)

Hansen, D.R.

1996-01-01

This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in Tank 241-SY-103. This report supports the requirements of the Tri-Party Agreement Milestone M-44-09

syNErgy: A Case Study in Workforce Curriculum Development

Science.gov (United States)

Killingsworth, John; Grosskopf, Kevin R.

2013-01-01

With high unemployment and structural changes to industry, workforce development in the United States is a growing concern. Many semiskilled workers lack knowledge, skills, and abilities to be competitive for reemployment to green jobs. Nebraska's syNErgy research grant was introduced to address the training needs of unemployed and underemployed…

Acceptance Test Procedure: SY101 air pallet system

International Nuclear Information System (INIS)

Koons, B.M.

1995-01-01

The purpose of this test procedure is to verify that the system(s) procured to load the SY-101 Mitigation Test Pump package fulfills its functional requirements. It will also help determine the man dose expected due to handling of the package during the actual event. The scope of this procedure focuses on the ability of the air pallets and container saddles to carry the container package from the new 100 foot concrete pad into 2403-WD where it will be stored awaiting final disposition. This test attempts to simulate the actual event of depositing the SY-101 hydrogen mitigation test pump into the 2403-WD building. However, at the time of testing road modifications required to drive the 100 ton trailer into CWC were not performed. Therefore a flatbed trailer will be use to transport the container to CWC. The time required to off load the container from the 100 ton trailer will be recorded for man dose evaluation on location. The cranes used for this test will also be different than the actual event. This is not considered to be an issue due to minimal effects on man dose

Morphological Differentiation Towards Neuronal Phenotype of SH-SY5Y Neuroblastoma Cells by Estradiol, Retinoic Acid and Cholesterol.

Science.gov (United States)

Teppola, Heidi; Sarkanen, Jertta-Riina; Jalonen, Tuula O; Linne, Marja-Leena

2016-04-01

Human SH-SY5Y neuroblastoma cells maintain their potential for differentiation and regression in culture conditions. The induction of differentiation could serve as a strategy to inhibit cell proliferation and tumor growth. Previous studies have shown that differentiation of SH-SY5Y cells can be induced by all-trans-retinoic-acid (RA) and cholesterol (CHOL). However, signaling pathways that lead to terminal differentiation of SH-SY5Y cells are still largely unknown. The goal of this study was to examine in the RA and CHOL treated SH-SY5Y cells the additive impacts of estradiol (E2) and brain-derived neurotrophic factor (BDNF) on cell morphology, cell population growth, synaptic vesicle recycling and presence of neurofilaments. The above features indicate a higher level of neuronal differentiation. Our data show that treatment for 10 days in vitro (DIV) with RA alone or when combined with E2 (RE) or CHOL (RC), but not when combined with BDNF (RB), significantly (p differentiation.

Syöpäpotilaiden toiveet hoitoympäristöstä päiväsairaalassa

OpenAIRE

Mäki, Saara; Ojalainen, Kati

2015-01-01

Tämän opinnäytetyön tarkoituksena oli selvittää syöpäpotilaiden toiveita päiväsairaalan fyysisestä hoitoympäristöstä. Tavoitteena oli saada selville ideaali päiväsairaalatila, jossa potilaat kokevat olonsa mahdollisimman turvalliseksi ja miellyttäväksi. Tämän opinnäytetyön tuloksia voidaan hyödyntää suunnitellessa uuden syöpäsairaalan päiväosastojen hoitotiloja. Opinnäytetyö on toteutettu yhteistyössä Helsingin ja Uudenmaan sairaanhoitopiirin HYKS :in Syöpäkeskuksen kanssa. Tämän opinnäyt...

The SH-SY5Y cell line in Parkinson's disease research: a systematic review.

Science.gov (United States)

Xicoy, Helena; Wieringa, Bé; Martens, Gerard J M

2017-01-24

Parkinson's disease (PD) is a devastating and highly prevalent neurodegenerative disease for which only symptomatic treatment is available. In order to develop a truly effective disease-modifying therapy, improvement of our current understanding of the molecular and cellular mechanisms underlying PD pathogenesis and progression is crucial. For this purpose, standardization of research protocols and disease models is necessary. As human dopaminergic neurons, the cells mainly affected in PD, are difficult to obtain and maintain as primary cells, current PD research is mostly performed with permanently established neuronal cell models, in particular the neuroblastoma SH-SY5Y lineage. This cell line is frequently chosen because of its human origin, catecholaminergic (though not strictly dopaminergic) neuronal properties, and ease of maintenance. However, there is no consensus on many fundamental aspects that are associated with its use, such as the effects of culture media composition and of variations in differentiation protocols. Here we present the outcome of a systematic review of scientific articles that have used SH-SY5Y cells to explore PD. We describe the cell source, culture conditions, differentiation protocols, methods/approaches used to mimic PD and the preclinical validation of the SH-SY5Y findings by employing alternative cellular and animal models. Thus, this overview may help to standardize the use of the SH-SY5Y cell line in PD research and serve as a future user's guide.

Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research.

Science.gov (United States)

Cheung, Yuen-Ting; Lau, Way Kwok-Wai; Yu, Man-Shan; Lai, Cora Sau-Wan; Yeung, Sze-Chun; So, Kwok-Fai; Chang, Raymond Chuen-Chung

2009-01-01

Human neuroblastoma SH-SY5Y is a dopaminergic neuronal cell line which has been used as an in vitro model for neurotoxicity experiments. Although the neuroblastoma is usually differentiated by all-trans-retinoic acid (RA), both RA-differentiated and undifferentiated SH-SY5Y cells have been used in neuroscience research. However, the changes in neuronal properties triggered by RA as well as the subsequent responsiveness to neurotoxins have not been comprehensively studied. Therefore, we aim to re-evaluate the differentiation property of RA on this cell line. We hypothesize that modulation of signaling pathways and neuronal properties during RA-mediated differentiation in SH-SY5Y cells can affect their susceptibility to neurotoxins. The differentiation property of RA was confirmed by showing an extensive outgrowth of neurites, increased expressions of neuronal nuclei, neuron specific enolase, synaptophysin and synaptic associated protein-97, and decreased expression of inhibitor of differentiation-1. While undifferentiated SH-SY5Y cells were susceptible to 6-OHDA and MPP+, RA-differentiation conferred SH-SY5Y cells higher tolerance, potentially by up-regulating survival signaling, including Akt pathway as inhibition of Akt removed RA-induced neuroprotection against 6-OHDA. As a result, the real toxicity cannot be revealed in RA-differentiated cells. Therefore, undifferentiated SH-SY5Y is more appropriate for studying neurotoxicity or neuroprotection in experimental Parkinson's disease research.

Operational experience in mitigating flammable gas releases from Hanford Site Tank 241-SY-101

International Nuclear Information System (INIS)

Lentsch, J.W.; Babad, H.; Kirch, N.W.

1995-01-01

Flammable gases consisting of hydrogen, nitrous oxide, ammonia, and methane are periodically released from Hanford Site waste tank 241-SY-101 at concentrations above the flammable limit. A large mixer pump installed in the tank in 1993 has effectively mitigated this problem by continuously releasing small amounts of the flammable gases at the rate they are generated. Tank 241-SY-101 is also equipped with multiple high-sensitivity gas monitoring systems and level detection systems to measure the quantity of gas that is retained in and released from the waste

Changes in America's Public School Facilities: From School Year 1998-99 to School Year 2012-13. Stats in Brief. NCES 2016-074

Science.gov (United States)

Bahr, Steven; Sparks, Dinah

2016-01-01

This Statistics in Brief summarizes the changes from the 1998-99 to the 2012-13 school years in the average age of public schools, ratings of satisfaction of the environmental quality of school facilities, the cost to put school buildings in good overall condition, and short-range plans to improve school facilities. In addition to providing…

Yksityisen syöpäsairaalan tietoturvakartoitus

OpenAIRE

Kitunen, Martti

2014-01-01

Tämän opinnäytetyön aiheena oli kartoittaa yksityisen syöpäsairaalan tietoturvallisuutta. Työssä haluttiin tuoda esille tietoturvallisuutta sairaalan näkökulmasta. Tutkimuksen teoriaosuudessa osuudessa perehdyttiin tietoturvan osa-alueisiin ja -käsitteisiin. Työssä tarkasteltiin tietoturvallisuuden merkitystä yrityksille, sekä mahdollisia tietoturvauhkia. Lisäksi käytiin läpi tietoturvan arviointiin käytettäviä teknisiä ratkaisuja. Tutkimuksen tavoitteena oli kartoittaa Docrateen tietotur...

Tank SY-102 waste retrieval assessment: Rheological measurements and pump jet mixing simulations

International Nuclear Information System (INIS)

Onishi, Y.; Shekarriz, R.; Recknagle, K.P.

1996-09-01

Wastes stored in Hanford Tank 241-SY-102 are planned to be retrieved from that tank and transferred to 200 East Area through the new pipeline Replacement Cross Site Transfer System (RCSTS). Because the planned transfer of this waste will use the RCSTS, the slurry that results from the mobilization and retrieval operations must meet the applicable waste acceptance criteria for this system. This report describes results of the second phase (the detailed assessment) of the SY-102 waste retrieval study, which is a part of the efforts to establish a technical basis for mobilization of the slurry, waste retrieval, and slurry transport. Hanford Tank 241-SY-102 is located in the SY Tank Farm in the Hanford Site's 200 West Area. It was built in 1977 to serve as a feed tank for 242-S Evaporator/Crystallizer, receiving supernatant liquid from S, SX, T, and U tank farms. Since 1981, the primary sources of waste have been from 200 West Area facilities, e.g., T-Plant decontamination operations, Plutonium Finishing Plant operations, and the 222-S Laboratory. It is the only active-service double-shell tank (DST) in the 200 West Area and is used as the staging tank for cross-site transfers to 200 East Area DSTs. The tank currently stores approximately 470 kL (125 kgal) of sludge wastes from a variety of sources including the Plutonium Finishing Plant, T-Plant, and the 222-S Laboratory. In addition to the sludge, approximately twice this amount (about 930 kL) of dilute, noncomplexed waste forms a supernatant liquid layer above the sludge

Microstructure and phase transformations in laser clad CrxSy/Ni coating on H13 steel

Science.gov (United States)

Lei, Yiwen; Sun, Ronglu; Tang, Ying; Niu, Wei

2015-03-01

Laser cladding was carried out onto H13 steel with preplaced NiCrBSi+Ni/MoS2 powders using CO2 laser under the optimized experimental parameters of laser power 2 kW, scanning velocity 6 mm/s and laser beam diameter 3 mm. An X-ray diffractometer and scanning electron microscope with energy dispersive spectroscopy were applied to analyze the microstructure and phase compositions of the coating. Thermodynamic calculation was performed with Thermo-Calc software on the basis of a commercially available Ni-based Alloys' database. The experimental results show that MoS2 decomposed and S reacted with Cr to form nonstoichiometric CrxSy during the laser cladding process. The coating consists of spherical CrxSy particles, primary γ-Ni dendrite, interdendritic eutectic (γ-Ni+NiMo) and precipitated NiMo. The precipitated NiMo was fine and uniformly distributed in primary γ-Ni dendrite. The calculated results and experimental data indicate that the solidification process in the coating during laser cladding process was liquid→liquid+CrxSy→ liquid+CrxSy+γ-Ni→liquid+CrxSy+γ-Ni+ eutectic (γ-Ni+NiMo). A solid state phase transformation (fine and uniformly distributed NiMo precipitated from γ-Ni) occurred after the solidification process. The calculations agree well with the experimental data and it is helpful to understand the phase transformation and microstructure evolution in the coating.

Laboratory testing of ozone oxidation of Hanford Site waste from Tank 241-SY-101

International Nuclear Information System (INIS)

Delegard, C.H.; Stubbs, A.M.; Bolling, S.D.

1993-01-01

Ozone was investigated as a reagent to oxidize and destroy organic species present in simulated and genuine waste from Hanford Site Tank 241-SY-101 (Tank 101-SY). Two high-shear mixing apparatus were tested to perform the gas-to-solution mass transfer necessary to achieve efficient use of the ozone reagent. Oxidations of nitrite (to form nitrate) and organic species were observed. The organics oxidized to form carbonate and oxalate as well as nitrate and nitrogen gas from nitrogen associated with the organic. oxidations of metal species also were observed directly or inferred by solubilities. The chemical reaction stoichiometries were consistent with reduction of one oxygen atom per ozone molecule. Acetate, oxalate, and formate were found to comprise about 40% of the genuine waste's total organic carbon (TOC) concentration. Ozonation was found to be chemically feasible for destroying organic species (except oxalate) present in the wastes in Tank 101-SY. The simulated waste formulation used in these studies credibly modelled the ozonation behavior of the genuine waste

Acceptance test procedure, 241-SY-101/241-C-106 shot loading system

International Nuclear Information System (INIS)

Ostrom, M.J.

1994-01-01

This Acceptance Test Procedure is for the 241-SY-101/241-C-106 Shot Loading System. The procedure will test the components of the Shot Loading System and its capability of adequately loading shot into the annular space of the Container. The loaded shot will provide shielding as required for transporting and storage of a contaminated pump after removal from the tank. This test serves as verification that the SLS is acceptable for use in the pump removal operations for Tanks 241-SY-101, 241-C-106 and 241-AY-102. The pump removal operation for these three tanks will be performed by two different organizations with different equipment, but the Shot Loading System will be compatible between the two operations

The effects of heating and dilution on the rheological and physical properties of Tank 241-SY-101 waste

International Nuclear Information System (INIS)

Tingey, J.M.; Bredt, P.R.; Shade, E.H.

1994-10-01

Of the 177 high-level waste underground storage tanks at the Hanford Site, 25 have been identified as being potentially capable of generating and releasing flammable gas. Tank 241-SY-101 has exhibited periodic releases of gas, and in some cases the gas released has exceeded the lower flammable gas limit. The components of the released gas from Tank 241-SY-101 are hydrogen, nitrous oxide, nitrogen, ammonia, carbon monoxide, and methane. A mitigation strategy that may effectively reduce the retention and release of these gases and the release of flammable gases is dilution coupled with eating of the tank wastes. The purpose of this work was to determine changes in rheological and physical properties caused by heating and dilution of actual 241-SY-101 waste. In May and December 1991, following periodic gas releases, samples of the waste in Tank 241-SY-101 were obtained. Current work quantified the effects of heating coupled with NaOH dilution of a combination of waste samples from Tank 241-SY-101 characteristic of a non-convective layer. The experimental approach and results of this heating and dilution study on Tank 241-SY-101 waste samples are described in Sections 2 and 3, respectively. In Section 3.1, a discussion of the rheological properties of the waste as a function of shearing forces, volume percent dilution, and temperature is presented. In Section 3.2, the physical properties of the waste dilutions are described, including the densities of the slurry, filtered solids, and filtrate; the settling behavior; and the percent filtered solids in the composite sample and each of the composite dilutions. A brief discussion of the results and uncertainties is given is Section 3.3. The conclusions of this investigation are reported in Section 4

The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells

Directory of Open Access Journals (Sweden)

Ana O. Pires

2014-01-01

Full Text Available The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs isolated from human bone-marrow (BMSCs and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y. For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM from the MSCs populations referred above. Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells. SH-SY5Y cells viability assessment revealed that the secretome of BMSCs and HUCPVCs, in the form of CM, was able to induce their survival. Moreover, immunocytochemical experiments showed that CM from both MSCs was capable of inducing neuronal differentiation of SH-SY5Y cells. Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. Overall, our results show that the secretome of both BMSCs and HUCPVCs was capable of supporting SH-SY5Y cells survival and promoting their differentiation towards a neuronal phenotype.

Assessment of the potential for ammonium nitrate formation and reaction in Tank 241-SY-101

International Nuclear Information System (INIS)

Pederson, L.R.; Bryan, S.A.

1994-08-01

Two principal scenarios by which ammonium nitrate may be formed were considered: (a) precipitation of ammonium nitrate in the waste, and (b) ammonium nitrate formation via the gas phase reaction of ammonia and nitrogen dioxide. The first of these can be dismissed because ammonium ions, which are necessary for ammonium nitrate precipitation, can exist only in negligibly small concentrations in strongly alkaline solutions. Gas phase reactions between ammonia, nitrogen dioxide, and water vapor in the gas phase represent the most likely means by which ammonium nitrate aerosols could be formed in Tank 241-SY-101. Predicted ammonium nitrate formation rates are largely controlled by the concentration of nitrogen dioxide. This gas has not been detected among those gases vented from the wastes using Fourier Transform Infrared Spectrometry (FTIR) or mass spectrometry. While detection limits for nitrogen dioxide have not been established experimentally, the maximum concentration of nitrogen dioxide in the gas phase in Tank 241-SY-101 was estimated at 0.1 ppm based on calculations using the HITRAN data base and on FTIR spectra of gases vented from the wastes. At 50 C and with 100 ppm ammonia also present, less than one gram of ammonium nitrate per year is estimated to be formed in the tank. To date, ammonium nitrate has not been detected on HEPA filters in the ventilation system, so any quantity that has been formed in the tank must be quite small, in good agreement with rate calculations. The potential for runaway exothermic reactions involving ammonium nitrate in Tank 241-SY-101 is minimal. Dilution by non-reacting waste components, particularly water, would prevent hazardous exothermic reactions from occurring within the waste slurry, even if ammonium nitrate were present. 41 refs

Acceptance test procedure for SY Tank Farm replacement exhauster unit

Energy Technology Data Exchange (ETDEWEB)

Becken, G.W.

1994-12-16

The proper functioning of a new 241-SY Tank Farm replacement exhauster will be acceptance tested, to establish operability and to provide an operational baseline for the equipment. During this test, a verification of all of the alarm and control circuits associated with the exhaust, which provide operating controls and/or signals to local and remote alarm/annunciator panels, shall be performed. Test signals for sensors that provide alarms, warnings, and/or interlocks will be applied to verify that alarm, warning, and interlock setpoints are correct. Alarm and warning lights, controls, and local and remote readouts for the exhauster will be verified to be adequate for proper operation of the exhauster. Testing per this procedure shall be conducted in two phases. The first phase of testing, to verify alarm, warning, and interlock setpoints primarily, will be performed in the MO-566 Fab Shop. The second phase of testing, to verify proper operation and acceptable interface with other tank farm systems, will be conducted after the exhauster and all associated support and monitoring equipment have been installed in the SY Tank Farm. The exhauster, which is mounted on a skid and which will eventually be located in the SY tank farm, receives input signals from a variety of sensors mounted on the skid and associated equipment. These sensors provide information such as: exhauster system inlet vacuum pressure; prefilter and HEPA filter differential pressures; exhaust stack sampler status; exhaust fan status; system status (running/shut down); and radiation monitoring systems status. The output of these sensors is transmitted to the exhauster annunciator panel where the signals are displayed and monitored for out-of-specification conditions.

Protective Effect of Edaravone Against Aβ25-35-Induced Mitochondrial Oxidative Damage in SH-SY5Y Cells.

Science.gov (United States)

Zhang, G-L; Zhang, L; Guo, Y-Y; Ma, Z-L; Wang, H-Y; Li, T; Liu, J; Du, Y; Yao, L; Li, T-T; Du, J-M

2017-05-20

Amyloid-β (Aβ)-induced oxidative stress plays an important role in the pathogenesis of Alzheimer's disease (AD). Recent studies show that Aβ accumulation may lead to mitochondrial oxidative damage. In the present study, we investigated the protective effect of edaravone on mitochondrial damage in SH-SY5Y cells treated with Aβ25-35. SH-SY5Y cells were pre-treated with 20, 40 or 80 μM edaravone before treatment with 25 μM Aβ25-35. After 24h cell culture, cellular apoptosis, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), ATP levels and mitochondrial morphology were evaluated. SH-SY5Y cells exposed to Aβ25-35 had high levels of apoptosis and ROS; loss of ΔΨm, decreased ATP levels and presence of mitochondrial swelling. However, these effects were significantly inhibited by edaravone pre-treatment. These results indicate that edaravone prevents mitochondria oxidative damage caused by Aβ in SH-SY5Y cells, which suggests that it may have potential clinical application in AD therapy.

Békésy's contributions to our present understanding of sound conduction to the inner ear.

Science.gov (United States)

Puria, Sunil; Rosowski, John J

2012-11-01

In our daily lives we hear airborne sounds that travel primarily through the external and middle ear to the cochlear sensory epithelium. We also hear sounds that travel to the cochlea via a second sound-conduction route, bone conduction. This second pathway is excited by vibrations of the head and body that result from substrate vibrations, direct application of vibrational stimuli to the head or body, or vibrations induced by airborne sound. The sensation of bone-conducted sound is affected by the presence of the external and middle ear, but is not completely dependent upon their function. Measurements of the differential sensitivity of patients to airborne sound and direct vibration of the head are part of the routine battery of clinical tests used to separate conductive and sensorineural hearing losses. Georg von Békésy designed a careful set of experiments and pioneered many measurement techniques on human cadaver temporal bones, in physical models, and in human subjects to elucidate the basic mechanisms of air- and bone-conducted sound. Looking back one marvels at the sheer number of experiments he performed on sound conduction, mostly by himself without the aid of students or research associates. Békésy's work had a profound impact on the field of middle-ear mechanics and bone conduction fifty years ago when he received his Nobel Prize. Today many of Békésy's ideas continue to be investigated and extended, some have been supported by new evidence, some have been refuted, while others remain to be tested. Copyright © 2012 Elsevier B.V. All rights reserved.

Short Chemical Ischemia Triggers Phosphorylation of eIF2α and Death of SH-SY5Y Cells but not Proteasome Stress and Heat Shock Protein Response in both SH-SY5Y and T98G Cells.

Science.gov (United States)

Klacanova, Katarina; Pilchova, Ivana; Klikova, Katarina; Racay, Peter

2016-04-01

Both translation arrest and proteasome stress associated with accumulation of ubiquitin-conjugated protein aggregates were considered as a cause of delayed neuronal death after transient global brain ischemia; however, exact mechanisms as well as possible relationships are not fully understood. The aim of this study was to compare the effect of chemical ischemia and proteasome stress on cellular stress responses and viability of neuroblastoma SH-SY5Y and glioblastoma T98G cells. Chemical ischemia was induced by transient treatment of the cells with sodium azide in combination with 2-deoxyglucose. Proteasome stress was induced by treatment of the cells with bortezomib. Treatment of SH-SY5Y cells with sodium azide/2-deoxyglucose for 15 min was associated with cell death observed 24 h after treatment, while glioblastoma T98G cells were resistant to the same treatment. Treatment of both SH-SY5Y and T98G cells with bortezomib was associated with cell death, accumulation of ubiquitin-conjugated proteins, and increased expression of Hsp70. These typical cellular responses to proteasome stress, observed also after transient global brain ischemia, were not observed after chemical ischemia. Finally, chemical ischemia, but not proteasome stress, was in SH-SY5Y cells associated with increased phosphorylation of eIF2α, another typical cellular response triggered after transient global brain ischemia. Our results showed that short chemical ischemia of SH-SY5Y cells is not sufficient to induce both proteasome stress associated with accumulation of ubiquitin-conjugated proteins and stress response at the level of heat shock proteins despite induction of cell death and eIF2α phosphorylation.

Syömishäiriökuntoutujien hoitopolku : Peruskouluikäisten psykofyysinen fysioterapia Tampereen kaupungilla

OpenAIRE

Mäenrinta, Ellen

2015-01-01

Syömishäiriöt ovat yleisiä nuorten psykiatrisia sairauksia ja useimmiten uhkaavat nuoren normaalia kehitystä. Fysioterapia voi kuulua syömishäiriötä sairastava lapsen tai nuoren hoitoon jo heti alusta alkaen yhteistyössä moniammatillisen tiimin kanssa. Fysioterapia toteutetaan psykofyysisestä näkökulmasta yksilön omien tarpeiden ja tavoitteiden mukaisesti. Fysioterapiassa käsitellään muun muassa sairastuneen vääristyneitä kokemuksia omasta kehostaan, kielteistä suhtautumista omaan ulkonäkööns...

Ortoreksia : Oppitunti syömishäiriöistä yläkoulun 9.-luokkalaisille

OpenAIRE

Suvanto, Minna; Tanninen, Milla

2015-01-01

Elinympäristömme luo ihmisille helposti monenlaisia paineita. Sekä kauneudelle että terveydelle kuin ulkonäöllekin on luotu tietynlaiset ihanteet ja esikuvia on tarjolla paljon kaikenikäisille. Samalla ravitsemussuositukset muuttuvat jatkuvasti vallitsevan ajan myötä. Syömishäiriöt ovat käsitteenä melko yleisiä. Tunnetuimmat niistä ovat anoreksia, bulimia ja BED. Tämä opinnäytetyö käsittelee kyseisten syömishäiriöiden peruspiirteitä, etiologiaa ja parantumisvaiheita. Varsinaiseksi aiheeks...

Historical trends in tank 241-SY-101 waste temperatures and levels

International Nuclear Information System (INIS)

Antoniak, Z.I.

1993-09-01

The gas release and fluctuating level of the waste in tank 241-SY-101 have prompted more detailed interest in its historical behavior, in hopes of achieving a better understanding of its current status. To examine the historical behavior, essentially all of the tank waste temperature and level data record has been retrieved, examined, and plotted in various ways. To aid in interpreting the data, the depth of the non-convective waste layer was estimated by using a least-squares Chebyshev approximation to the temperatures. This report documents the retrieval critical examination, and graphic presentation of 241-SY-101 temperature and waste level histories. The graphic presentations clearly indicate a tank cooling trend that has become precipitous since late 1991. The plots also clearly show the decreasing frequency of waste gas release events, increasing height of the non-convective layer, and larger level drops per event

SyPRID sampler: A large-volume, high-resolution, autonomous, deep-ocean precision plankton sampling system

Science.gov (United States)

Billings, Andrew; Kaiser, Carl; Young, Craig M.; Hiebert, Laurel S.; Cole, Eli; Wagner, Jamie K. S.; Van Dover, Cindy Lee

2017-03-01

The current standard for large-volume (thousands of cubic meters) zooplankton sampling in the deep sea is the MOCNESS, a system of multiple opening-closing nets, typically lowered to within 50 m of the seabed and towed obliquely to the surface to obtain low-spatial-resolution samples that integrate across 10 s of meters of water depth. The SyPRID (Sentry Precision Robotic Impeller Driven) sampler is an innovative, deep-rated (6000 m) plankton sampler that partners with the Sentry Autonomous Underwater Vehicle (AUV) to obtain paired, large-volume plankton samples at specified depths and survey lines to within 1.5 m of the seabed and with simultaneous collection of sensor data. SyPRID uses a perforated Ultra-High-Molecular-Weight (UHMW) plastic tube to support a fine mesh net within an outer carbon composite tube (tube-within-a-tube design), with an axial flow pump located aft of the capture filter. The pump facilitates flow through the system and reduces or possibly eliminates the bow wave at the mouth opening. The cod end, a hollow truncated cone, is also made of UHMW plastic and includes a collection volume designed to provide an area where zooplankton can collect, out of the high flow region. SyPRID attaches as a saddle-pack to the Sentry vehicle. Sentry itself is configured with a flight control system that enables autonomous survey paths to low altitudes. In its verification deployment at the Blake Ridge Seep (2160 m) on the US Atlantic Margin, SyPRID was operated for 6 h at an altitude of 5 m. It recovered plankton samples, including delicate living larvae, from the near-bottom stratum that is seldom sampled by a typical MOCNESS tow. The prototype SyPRID and its next generations will enable studies of plankton or other particulate distributions associated with localized physico-chemical strata in the water column or above patchy habitats on the seafloor.

Acquisition and reduction of data obtained from Tank 101-SY in-situ ball rheometer

International Nuclear Information System (INIS)

Shepard, C.L.; Chieda, M.A.; Kirihara, L.J.

1994-12-01

Development of the ball rheometer to measure rheological properties and density of the waste in Hanford Tank 241-SY-101 will be completed around September 1994. Since the ball rheometer project began, a mixer pump has been installed in this tank, and by all accounts this pump has been very successful at mitigating the flammable gas problem associated with Tank 101-SY. Present plans now call for the use of mixer pumps in several other tanks. The ball rheometer will serve as a diagnostic tool for judging the effectiveness of mixing in Tank 101-SY and others and will be one of few in-situ probes available for diagnostic measurements. The in-situ data collection strategy and the methods of data analysis and reduction are presented in this final report concerning this instrument. It is believed that a generalized Bingham fluid model (Herschel-Bulkley fluid model) may be useful for describing at least some of the waste contained in Tank 101-SY, and data obtained in the tank will initially be reduced using this fluid model. The single largest uncertainty in the determination of the drag force on the ball is the drag force which will be experienced by the cable attached to the ball. This drag can be a substantial fraction of the total drag when the ball is deep within the tank. Careful accounting of the cable drag will be important in the reduction of the data. The data collection strategy allows the determination of the waste fluid rheology both in the undisturbed state and after it has been disturbed by the ball. Fluid density will be measured at regular intervals

Transport of Tank 241-SY-101 Waste Slurry: Effects of Dilution and Temperature on Critical Pipeline Velocity

International Nuclear Information System (INIS)

KP Recknagle; Y Onishi

1999-01-01

This report presents the methods and results of calculations performed to predict the critical velocity and pressure drop required for the two-inch pipeline transfer of solid/liquid waste slurry from underground waste storage Tank 241-SY-101 to Tank 241-SY- 102 at the Hanford Site. The effects of temperature and dilution on the critical velocity were included in the analysis. These analyses show that Tank 241-SY-101 slurry should be diluted with water prior to delivery to Tank 241-SY-102. A dilution ratio of 1:1 is desirable and would allow the waste to be delivered at a critical velocity of 1.5 ft/sec. The system will be operated at a flow velocity of 6 ft/sec or greater therefore, this velocity will be sufficient to maintain a stable slurry delivery through the pipeline. The effect of temperature on the critical velocity is not a limiting factor when the slurry is diluted 1:1 with water. Pressure drop at the critical velocity would be approximately two feet for a 125-ft pipeline (or 250-ft equivalent straight pipeline). At 6 ft/sec, the pressure drop would be 20 feet over a 250-ft equivalent straight pipeline

Similarity analysis applied to the design of scaled tests of hydraulic mitigation methods for Tank 241-SY-101

International Nuclear Information System (INIS)

Liljegren, L.M.

1993-02-01

The episodic gas releases from Tank 241-SY-101 (SY-101) pose a potential safety hazard. It is thought that gas releases occur because gases are generated and trapped in layers of settled solids located at the bottom of the tank. This document focuses on issues associated with testing of hydraulic mitigation technologies proposed for SY-101. The basic assumption underlying the concept of hydraulic mitigation is that mobilization or maintained suspension of the solids settled in the bottom of the tank wig prevent gas accumulation. Engineering of hydraulic technologies will require testing to determine the operating parameters required to mobilize the solids and to maintain these solids in suspension. Because full scale testing is extremely expensive (even when possible), scaled tests are needed to assess the merit of the proposed technologies and to provide data for numerical or analytical modeling. This research is conducted to support testing and evaluation of proposed hydraulic mitigation concepts only. The work here is oriented towards determining the jet velocities, nozzle sizes, and other operating parameters required to mobilize the settled solids in SY- 101 and maintain them in suspension

Neuroprotective Effects of Germinated Brown Rice against Hydrogen Peroxide Induced Cell Death in Human SH-SY5Y Cells

Directory of Open Access Journals (Sweden)

Shahid Iqbal

2012-08-01

Full Text Available The neuroprotective and antioxidative effects of germinated brown rice (GBR, brown rice (BR and commercially available γ-aminobutyric acid (GABA against cell death induced by hydrogen peroxide (H₂O₂ in human neuroblastoma SH-SY5Y cells have been investigated. Results show that GBR suppressed H₂O₂-mediated cytotoxicity and induced G0/G1 phase cell cycle arrest in SH-SY5Y cells. Moreover, GBR reduced mitochondrial membrane potential (MMP and prevented phosphatidylserine (PS translocation in SH-SY5Y cells, key features of apoptosis, and subsequent cell death. GBR exhibited better neuroprotective and antioxidative activities as compared to BR and GABA. These results indicate that GBR possesses high antioxidative activities and suppressed cell death in SH-SY5Y cells by blocking the cell cycle re-entry and apoptotic mechanisms. Therefore, GBR could be developed as a value added functional food to prevent neurodegenerative diseases caused by oxidative stress and apoptosis.

System Design Description for the SY-101 Hydrogen Mitigation Test Project Data Acquisition and Control System (DACS-1)

Energy Technology Data Exchange (ETDEWEB)

ERMI, A.M.

1999-08-25

This document describes the hardware and software of the computer subsystems for the Data Acquisition and Control System (DACS) used in mitigation tests conducted on waste tank 241-SY-101 at the Hanford Nuclear Reservation, The original system was designed and implemented by LANL, supplied to WHC, and turned over to LMHC for operation. In 1999, the hardware and software were upgraded to provide a state-of-the-art, Year-2000 compliant system.

The Syed temporary interstitial iridium gynaecological implant: an inverse planning system

International Nuclear Information System (INIS)

Fung, Albert Y.C.

2002-01-01

Patients with advanced gynaecological cancer are often treated with a temporary interstitial implant using the Syed template and Ir-192 ribbons at the Memorial Sloan-Kettering Cancer Center. Urgency in planning is great. We created a computerized inverse planning system for the Syed temporary gynaecological implant, which optimized the ribbon strengths a few seconds after catheter digitization. Inverse planning was achieved with simulated annealing. We discovered that hand-drawn target volumes had drawbacks; hence instead of producing a grid of points based on target volume, the optimization points were generated directly from the catheter positions without requiring an explicit target volume. Since all seeds in the same ribbon had the same strength, the minimum doses were located at both ends of the implant. Optimization points generated at both ends ensured coverage of the whole implant. Inverse planning took only a few seconds, and generated plans that provide a good starting point for manual improvement. (author)

SAFETY ANALYSIS APPROACH TO TANK 241-SY-101 REMEDIATION ACTIVITIES

International Nuclear Information System (INIS)

RYAN, G.W.

2000-01-01

An Unreviewed Safety Question was declared related to the unexplained waste surface level growth in high-level radioactive waste storage Tank 241-SY-101 at the Hanford Site in Richland, Washington. Because the waste surface level in Tank 241-SY-101 was growing in a manner inconsistent with previous behavior, the following issues of concern were recognized: (1) The continually rising surface level had the potential to reach physical encumbrances or limits within the tank (e.g., instrumentation, cameras, established Authorization Basis limits, and the double containment boundary) and the potential to significantly change the consequences of previously analyzed accidents (e.g., flammable gas deflagrations). (2) The presence of new hazards because of significant quantities of flammable gas retained in the crust (e.g., crust collapse gas-release events). (3) The potential to inhibit information gathering related to the existing hazards in the tank (e.g., unable to determine surface level to assess the potential for large gas releases). In response to this situation, a Contractor Project Team, which included Department of Energy representation, was formed to constructively address the issue. The team was responsible for developing and evaluating remediation options and executing the chosen option for remediating the surface level rise issue for Tank 241-SY-101. From an Authorization Basis perspective, the following important aspects will be discussed in this paper: (1) The integrated nature of the Project Team. The team consisted of all the organizations necessary to ensure that the time available to remediate Tank 241-SY-101 was effectively used. Most notable is the connectivity of the Nuclear Safety and Licensing organization with the Engineering, Design, and Operations organizations. (2) The ability of the safety analysis support to adjust to and address evolving Project Team goals and dynamic tank conditions. (3) Due to the urgency to mitigate this developing issue

Characterization of the novel dimethyl sulfide-degrading bacterium Alcaligenes sp. SY1 and its biochemical degradation pathway

Energy Technology Data Exchange (ETDEWEB)

Sun, Yiming; Qiu, Jiguo; Chen, Dongzhi; Ye, Jiexu; Chen, Jianmeng, E-mail: jchen@zjut.edu.cn

2016-03-05

Highlights: • A novel efficient DMS-degrading bacterium Alcaligenes sp. SY1 was identified. • A RSM was applied to optimize incubation condition of Alcaligenes sp. SY1. • SIP was applied as C{sup 13} labelled DMS to trace intermediates during DMS degradation. • Kinetics of DMS degradation via batch experiment was revealed. • Carbon and sulfur balance were analyzed during DMS degradation process. - Abstract: Recently, the biodegradation of volatile organic sulfur compounds (VOSCs) has become a burgeoning field, with a growing focus on the reduction of VOSCs. The reduction of VOSCs encompasses both organic emission control and odor control. Herein, Alcaligenes sp. SY1 was isolated from active sludge and found to utilize dimethyl sulfide (DMS) as a growth substrate in a mineral salt medium. Response surface methodology (RSM) analysis was applied to optimize the incubation conditions. The following conditions for optimal degradation were identified: temperature 27.03 °C; pH 7.80; inoculum salinity 0.84%; and initial DMS concentration 1585.39 μM. Under these conditions, approximately 99% of the DMS was degraded within 30 h of incubation. Two metabolic compounds were detected and identified by gas chromatography–mass spectrometry (GC–MS): dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS). The DMS degradation kinetics for different concentrations were evaluated using the Haldane–Andrews model and the pseudo first-order model. The maximum specific growth rate and degradation rate of Alcaligenes sp. SY1 were 0.17 h{sup −1} and 0.63 gs gx{sup −1} h{sup −1}. A possible degradation pathway is proposed, and the results suggest that Alcaligenes sp. SY1 has the potential to control odor emissions under aerobic conditions.

Acceptance test report for the 241-SY-101 Flexible Receiver Gamma Detector System

International Nuclear Information System (INIS)

Dowell, J.L.

1995-01-01

This Acceptance Test Report is for the 241-SY-101 Flexible Receiver Gamma Detector System. This test verified that the data logger and data converter for the gamma detector system functions as intended

Past in the Present: The Way Parents Remember Their Own School Years Relates to the Way They Participate in Their Child's Schooling and Remember His/Her School Years

Science.gov (United States)

Raty, Hannu

2011-01-01

This study set out to explore the contribution of parents' own school memories to the way they remembered their child's school years and took part in his/her schooling. The respondents were a group of academically and vocationally educated fathers and mothers (N = 326), who participated in a full 9-year follow-up study of their child's schooling.…

MacSyFinder: a program to mine genomes for molecular systems with an application to CRISPR-Cas systems.

Directory of Open Access Journals (Sweden)

Sophie S Abby

Full Text Available Biologists often wish to use their knowledge on a few experimental models of a given molecular system to identify homologs in genomic data. We developed a generic tool for this purpose.Macromolecular System Finder (MacSyFinder provides a flexible framework to model the properties of molecular systems (cellular machinery or pathway including their components, evolutionary associations with other systems and genetic architecture. Modelled features also include functional analogs, and the multiple uses of a same component by different systems. Models are used to search for molecular systems in complete genomes or in unstructured data like metagenomes. The components of the systems are searched by sequence similarity using Hidden Markov model (HMM protein profiles. The assignment of hits to a given system is decided based on compliance with the content and organization of the system model. A graphical interface, MacSyView, facilitates the analysis of the results by showing overviews of component content and genomic context. To exemplify the use of MacSyFinder we built models to detect and class CRISPR-Cas systems following a previously established classification. We show that MacSyFinder allows to easily define an accurate "Cas-finder" using publicly available protein profiles.MacSyFinder is a standalone application implemented in Python. It requires Python 2.7, Hmmer and makeblastdb (version 2.2.28 or higher. It is freely available with its source code under a GPLv3 license at https://github.com/gem-pasteur/macsyfinder. It is compatible with all platforms supporting Python and Hmmer/makeblastdb. The "Cas-finder" (models and HMM profiles is distributed as a compressed tarball archive as Supporting Information.

241-SY Double Shell Tanks (DST) Integrity Assessment Report

International Nuclear Information System (INIS)

JENSEN, C.E.

1999-01-01

This report presents the results of the integrity assessment of the 241-SY double-shell tank farm facility located in the 200 West Area of the Hanford Site. The assessment included the design evaluation and integrity examinations of the tanks and concluded that the facility is adequately designed, is compatible with the waste, and is fit for use. Recommendations including subsequent examinations, are made to ensure the continued safe operation of the tanks

Antihypoxic effect of miR-24 in SH-SY5Y cells under hypoxia via downregulating expression of neurocan

Energy Technology Data Exchange (ETDEWEB)

Sun, Xingyuan, E-mail: sunxingyuan@sina.com; Ren, Zhanjun; Pan, Yunzhi; Zhang, Chenxin

2016-09-02

Hypoxia-induced apoptosis-related mechanisms involved in the brain damage following cerebral ischemia injury. A subset of the small noncoding microRNA (miRNAs) is regulated by tissue oxygen levels, and miR-24 was found to be activated by hypoxic conditions. However, the roles of miR-24 and its target gene in neuron are not well understood. Here, we validated miRNA-24 is down-regulated in patients with cerebral infarction. Hypoxia suppressed the expression of miR-24, but increased the expression of neurocan in both mRNA and protein levels in SH-SY5Y cells. MiR-24 mimics reduced the expression of neurocan, suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. By luciferase reporter assay, neurocan is validated a direct target gene of miR-24. Furthermore, knockdown of neurocan suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. Taken together, miR-24 overexpression or silencing of neurocan shows an antihypoxic effect in SH-SY5Y cells. Therefore, miR-24 and neurocan play critical roles in neuron cell apoptosis and are potential therapeutic targets for ischemic brain disease. - Highlights: • miR-24 and neurocan play critical roles in neuron cell apoptosis. • miR-24 and neurocan are potential therapeutic targets for ischemic brain disease. • Antihypoxic effect of miR-24 and neurocan in SH-SY5Y cells.

Salicin from Willow Bark can Modulate Neurite Outgrowth in Human Neuroblastoma SH-SY5Y Cells.

Science.gov (United States)

Wölfle, Ute; Haarhaus, Birgit; Kersten, Astrid; Fiebich, Bernd; Hug, Martin J; Schempp, Christoph M

2015-10-01

Salicin from willow bark has been used throughout centuries in China and Europe for the treatment of pain, headache, and inflammatory conditions. Recently, it could be demonstrated that salicin binds and activates the bitter taste receptor TAS2R16. Studies on rodent tissues showed the general expression of bitter taste receptors (TAS2Rs) in rodent brain. Here, we demonstrate the expression of hTAS2R16 in human neuronal tissues and the neuroblastoma cell line SH-SY5Y. The functionality was analyzed in the neuroblastoma cell line SH-SY5Y after stimulation with salicin, a known TAS2R16 agonist. In this setting salicin induced in SH-SY5Y cells phosphorylation of ERK and CREB, the key transcription factor of neuronal differentiation. PD98059, an inhibitor of the ERK pathway, as well as probenecid, a TAS2R16 antagonist, inhibited receptor phosphorylation as well as neurite outgrowth. These data show that salicin might modulate neurite outgrowth by bitter taste receptor activation. Copyright © 2015 John Wiley & Sons, Ltd.

Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.

Science.gov (United States)

Nishida, Yuichiro; Adati, Naoki; Ozawa, Ritsuko; Maeda, Aasami; Sakaki, Yoshiyuki; Takeda, Tadayuki

2008-10-28

SH-SY5Y cells exhibit a neuronal phenotype when treated with all-trans retinoic acid (RA), but the molecular mechanism of activation in the signalling pathway mediated by phosphatidylinositol 3-kinase (PI3K) is unclear. To investigate this mechanism, we compared the gene expression profiles in SK-N-SH cells and two subtypes of SH-SY5Y cells (SH-SY5Y-A and SH-SY5Y-E), each of which show a different phenotype during RA-mediated differentiation. SH-SY5Y-A cells differentiated in the presence of RA, whereas RA-treated SH-SY5Y-E cells required additional treatment with brain-derived neurotrophic factor (BDNF) for full differentiation. After exposing cells to a PI3K inhibitor, LY294002, we identified 386 genes and categorised these genes into two clusters dependent on the PI3K signalling pathway during RA-mediated differentiation in SH-SY5Y-A cells. Transcriptional regulation of the gene cluster, including 158 neural genes, was greatly reduced in SK-N-SH cells and partially impaired in SH-SY5Y-E cells, which is consistent with a defect in the neuronal phenotype of these cells. Additional stimulation with BDNF induced a set of neural genes that were down-regulated in RA-treated SH-SY5Y-E cells but were abundant in differentiated SH-SY5Y-A cells. We identified gene clusters controlled by PI3K- and TRKB-mediated signalling pathways during the differentiation of two subtypes of SH-SY5Y cells. The TRKB-mediated bypass pathway compensates for impaired neural function generated by defects in several signalling pathways, including PI3K in SH-SY5Y-E cells. Our expression profiling data will be useful for further elucidation of the signal transduction-transcriptional network involving PI3K or TRKB.

Children’s Day-Care Centre (EVE) and School kicked off the school year 2016-2017

CERN Multimedia

Staff Association

2016-01-01

It has been 54 years already, ever since the Nursery school was founded in March 1961, that the Staff Association together with the teachers, the managerial and the administrative staff, welcomes your children at the start of the school year. On Tuesday, 30 August 2016, the Children’s Day-Care Centre (EVE) and School opened its doors again for children between four months and six years old. The start of the school year was carried out gradually and in small groups to allow quality interaction between children, professionals and parents. This year, our structure will accommodate about 130 children divided between the nursery, the kindergarten and the school. Throughout the school year, the children will work on the theme of colours, which will be the common thread linking all our activities. Our team is comprised of 38 people: the headmistress, the deputy headmistress, 2 secretaries, 13 educators, 4 teachers, 11 teaching assistants, 2 nursery assistants and 4 canteen workers. The team is delighted...

Neuroprotective Effect of Arctigenin via Upregulation of P-CREB in Mouse Primary Neurons and Human SH-SY5Y Neuroblastoma Cells

Science.gov (United States)

Zhang, Nan; Wen, Qingping; Ren, Lu; Liang, Wenbo; Xia, Yang; Zhang, Xiaodan; Zhao, Dan; Sun, Dong; Hu, Yv; Hao, Haiguang; Yan, Yaping; Zhang, Guangxian; Yang, Jingxian; Kang, Tingguo

2013-01-01

Arctigenin (Arc) has been shown to act on scopolamine-induced memory deficit mice and to provide a neuroprotective effect on cultured cortical neurons from glutamate-induced neurodegeneration through mechanisms not completely defined. Here, we investigated the neuroprotective effect of Arc on H89-induced cell damage and its potential mechanisms in mouse cortical neurons and human SH-SY5Y neuroblastoma cells. We found that Arc prevented cell viability loss induced by H89 in human SH-SY5Y cells. Moreover, Arc reduced intracellular beta amyloid (Aβ) production induced by H89 in neurons and human SH-SY5Y cells, and Arc also inhibited the presenilin 1(PS1) protein level in neurons. In addition, neural apoptosis in both types of cells, inhibition of neurite outgrowth in human SH-SY5Y cells and reduction of synaptic marker synaptophysin (SYN) expression in neurons were also observed after H89 exposure. All these effects induced by H89 were markedly reversed by Arc treatment. Arc also significantly attenuated downregulation of the phosphorylation of CREB (p-CREB) induced by H89, which may contribute to the neuroprotective effects of Arc. These results demonstrated that Arc exerted the ability to protect neurons and SH-SY5Y cells against H89-induced cell injury via upregulation of p-CREB. PMID:24025424

Neuroprotective Effect of Arctigenin via Upregulation of P-CREB in Mouse Primary Neurons and Human SH-SY5Y Neuroblastoma Cells

Directory of Open Access Journals (Sweden)

Tingguo Kang

2013-09-01

Full Text Available Arctigenin (Arc has been shown to act on scopolamine-induced memory deficit mice and to provide a neuroprotective effect on cultured cortical neurons from glutamate-induced neurodegeneration through mechanisms not completely defined. Here, we investigated the neuroprotective effect of Arc on H89-induced cell damage and its potential mechanisms in mouse cortical neurons and human SH-SY5Y neuroblastoma cells. We found that Arc prevented cell viability loss induced by H89 in human SH-SY5Y cells. Moreover, Arc reduced intracellular beta amyloid (Aβ production induced by H89 in neurons and human SH-SY5Y cells, and Arc also inhibited the presenilin 1(PS1 protein level in neurons. In addition, neural apoptosis in both types of cells, inhibition of neurite outgrowth in human SH-SY5Y cells and reduction of synaptic marker synaptophysin (SYN expression in neurons were also observed after H89 exposure. All these effects induced by H89 were markedly reversed by Arc treatment. Arc also significantly attenuated downregulation of the phosphorylation of CREB (p-CREB induced by H89, which may contribute to the neuroprotective effects of Arc. These results demonstrated that Arc exerted the ability to protect neurons and SH-SY5Y cells against H89-induced cell injury via upregulation of p-CREB.

GLOBE and the Earth SySTEM Model in Teacher Preparation

Science.gov (United States)

Jabot, M.; Moore, J.; Dorofy, P.

2017-12-01

This presentation will share the growing body of work linking ArcMap and GLOBE and the Earth SySTEM approach in the development of preservice teachers. Our work is linking the power of ArcMap with the vast database of GLOBE in a unique way that links the power of geospatial technologies in shaping the planning for and delivery of science instruction in the P-5 classroom.

Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y

OpenAIRE

Nishida, Yuichiro; Adati, Naoki; Ozawa, Ritsuko; Maeda, Aasami; Sakaki, Yoshiyuki; Takeda, Tadayuki

2008-01-01

Abstract Background SH-SY5Y cells exhibit a neuronal phenotype when treated with all-trans retinoic acid (RA), but the molecular mechanism of activation in the signalling pathway mediated by phosphatidylinositol 3-kinase (PI3K) is unclear. To investigate this mechanism, we compared the gene expression profiles in SK-N-SH cells and two subtypes of SH-SY5Y cells (SH-SY5Y-A and SH-SY5Y-E), each of which show a different phenotype during RA-mediated differentiation. Findings SH-SY5Y-A cells diffe...

Design basis and requirements for 241-SY modular exhauster mechanical installation

International Nuclear Information System (INIS)

Kriskovich, J.R.

1994-01-01

A new ventilation system is being installed to serve as the K-1 primary exhauster. The existing K-1 primary exhauster will then become the backup. This ventilation system services waste tanks 241-SY-101, 102 and 103. The nominal flow rate through the ventilation system is 1,000 cfm. The new ventilation system will contain a moisture eliminator, a heater, a prefilter, two stages of HEPA filtration, an exhaust fan, a stack and stack sampling system. The purpose of this document is to serve as the design and functional requirements for the mechanical installation of the new 241-SY modular exhauster. The mechanical installation will include modifying the existing ductwork (i.e., installing a ''T'' to connect the new exhauster to the existing system), modifying the existing condensate drain lines to accommodate the new lines associated with the new exhauster, a maintenance platform near the stack of the new exhauster, guy wires and guy wire footings to support the stack of the new exhauster, as well as other miscellaneous tasks associated with the mechanical installation design effort

School Psychologists' Job Satisfaction: Ten Years Later

OpenAIRE

Worrell, Travis G.

2004-01-01

School Psychologistsâ Job Satisfaction: Ten Years Later (ABSTRACT) This study was designed to replicate nationwide surveys completed in 1982 and 1992. The purpose was to examine and describe the levels of job satisfaction and the relationship between the variables in a national sample of school psychologists belonging to the National Association of School Psychologists (NASP). The sample for this study consisted of respondents who reported being full-time school practitioners. ...

Regulation of nicotinic receptor subtypes following chronic nicotinic agonist exposure in M10 and SH-SY5Y neuroblastoma cells

DEFF Research Database (Denmark)

Warpman, U; Friberg, L; Gillespie, A

1998-01-01

investigated in human neuroblastoma SH-SY5Y cells (expressing alpha3, alpha5, beta2, and beta4 nAChR subunits). Nicotine exhibited a 14 times lower affinity for the nAChRs in SH-SY5Y cells as compared with M10 cells, whereas epibatidine showed similar affinities for the nAChRs expressed in the two cell lines...

Process Control Plan for Tank 241-SY-101 Surface Level Rise Remediation

International Nuclear Information System (INIS)

ESTEY, S.D.

1999-01-01

The tank 241-SY-101 transfer system was conceived and designed to address the immediate needs presented by rapidly changing waste conditions in tank 241-SY-101. Within approximately the last year, the waste in this tank has exhibited unexpected behavior (Rassat et al. 1999) in the form of rapidly increasing crust growth. This growth has been brought about by a rapidly increasing rate of gas entrapment within the crust. It has been conceived that the lack of crust agitation beginning upon the advent of mixer pump operations may have set-up a more consolidated, gas impermeable barrier when compared to a crust regularly broken up by the prior buoyant displacement events within the tank. The interim goals of the project are to: (1) protect the mixer pump operability (2) begin releasing gas from the crust, and (3) begin dissolving the crust and solids in the slurry layer. The final goals of the project (Final State) are to solve both the level growth and BD-GRE safety issues in this tank by achieving a condition of the waste such that no active measures are required to safely store the waste, i.e., crust and non convective layer are mostly dissolved, and therefore the mixer pump will no longer be needed to prevent BD-GREs in excess of 100% LFL. Transfers (which are designed to create space in the tank) and dilution (which will dissolve the solids) will accomplish this. Dissolution of solids will result in a release of gas retained by those solids and remove that volume of solids as a future retention site

Tank 101-SY Window E core sample: Interpretation of results

International Nuclear Information System (INIS)

Reynolds, D.A.

1993-02-01

A full depth core sample was taken for tank 241-SY-101 in December 1991 during a time period called ''Window E.'' This was the second full depth core sample from this tank during the year. The core had two major portions that are known as the convective zone and the nonconvective zone. A crust was on the top of tank but as poorly sampled. The analysis of the Window E core sample stressed segment composite chemical analysis instead of segment by segment as in Window C. Adiabatic calorimetry on samples from both cores showed a slow self heating reaction above 150 degrees C on dried samples. The exothermic events were milder than similar synthetic samples. The chemical and physical properties complemented the information from Window C. The Window E material from the convective zone was more viscous than the Window C convective zone material. The nonconvective zone viscosities were similar for both cores. Heating and dilution tests were made to test mitigation concepts

Tank 101-SY Window E core sample: Interpretation of results

Energy Technology Data Exchange (ETDEWEB)

Reynolds, D.A.

1993-02-01

A full depth core sample was taken for tank 241-SY-101 in December 1991 during a time period called ``Window E.`` This was the second full depth core sample from this tank during the year. The core had two major portions that are known as the convective zone and the nonconvective zone. A crust was on the top of tank but as poorly sampled. The analysis of the Window E core sample stressed segment composite chemical analysis instead of segment by segment as in Window C. Adiabatic calorimetry on samples from both cores showed a slow self heating reaction above 150{degrees}C on dried samples. The exothermic events were milder than similar synthetic samples. The chemical and physical properties complemented the information from Window C. The Window E material from the convective zone was more viscous than the Window C convective zone material. The nonconvective zone viscosities were similar for both cores. Heating and dilution tests were made to test mitigation concepts.

Extended School Year. Information Capsule. Volume 0910

Science.gov (United States)

Blazer, Christie

2010-01-01

Extended school years are being considered by districts around the country as educators search for new ways to raise student achievement. The addition of time to the school calendar is also supported by President Barack Obama, who recently stated that American students do not spend enough time in school. This Information Capsule addresses research…

Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death

Directory of Open Access Journals (Sweden)

Sandeep Vasant More

2017-05-01

Full Text Available Oxidative stress and apoptosis are the major mechanisms that induce dopaminergic cell death. Our study investigates the protective effects of atractylenolide-I (ATR-I on 1-methyl-4-phenylpyridinium (MPP+-induced cytotoxicity in human dopaminergic SH-SY5Y cells, as well as its underlying mechanism. Our experimental data indicates that ATR-I significantly inhibits the loss of cell viability induced by MPP+ in SH-SY5Y cells. To further unravel the mechanism, we examined the effect of ATR-I on MPP+-induced apoptotic cell death characterized by an increase in the Bax/Bcl-2 mRNA ratio, the release of cytochrome-c, and the activation of caspase-3 leading to elevated levels of cleaved poly(ADP-ribose polymerase (PARP resulting in SH-SY5Y cell death. Our results demonstrated that ATR-I decreases the level of pro-apoptotic proteins induced by MPP+ and also restored Bax/Bcl-2 mRNA levels, which are critical for inducing apoptosis. In addition, ATR-I demonstrated a significant increase in the protein expression of heme-oxygenase in MPP+-treated SH-SY5Y cells. These results suggest that the pharmacological effect of ATR-I may be, at least in part, caused by the reduction in pro-apoptotic signals and also by induction of anti-oxidant protein.

101-SY Dome pressure issues surrounding mitigation pump decontamination during removal

International Nuclear Information System (INIS)

Shaw, S.W.

1995-01-01

This document addresses issues related to use of the spraywands and ring used to decontaminate the mitigation pump installed in 101-SY. It has been determined that use of the wands will influence tank dome pressures as a function of ventilation system configuration, spray drop size, rinse water temperature, and rate at which spraywand flows are established

Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y

Directory of Open Access Journals (Sweden)

Sakaki Yoshiyuki

2008-10-01

Full Text Available Abstract Background SH-SY5Y cells exhibit a neuronal phenotype when treated with all-trans retinoic acid (RA, but the molecular mechanism of activation in the signalling pathway mediated by phosphatidylinositol 3-kinase (PI3K is unclear. To investigate this mechanism, we compared the gene expression profiles in SK-N-SH cells and two subtypes of SH-SY5Y cells (SH-SY5Y-A and SH-SY5Y-E, each of which show a different phenotype during RA-mediated differentiation. Findings SH-SY5Y-A cells differentiated in the presence of RA, whereas RA-treated SH-SY5Y-E cells required additional treatment with brain-derived neurotrophic factor (BDNF for full differentiation. After exposing cells to a PI3K inhibitor, LY294002, we identified 386 genes and categorised these genes into two clusters dependent on the PI3K signalling pathway during RA-mediated differentiation in SH-SY5Y-A cells. Transcriptional regulation of the gene cluster, including 158 neural genes, was greatly reduced in SK-N-SH cells and partially impaired in SH-SY5Y-E cells, which is consistent with a defect in the neuronal phenotype of these cells. Additional stimulation with BDNF induced a set of neural genes that were down-regulated in RA-treated SH-SY5Y-E cells but were abundant in differentiated SH-SY5Y-A cells. Conclusion We identified gene clusters controlled by PI3K- and TRKB-mediated signalling pathways during the differentiation of two subtypes of SH-SY5Y cells. The TRKB-mediated bypass pathway compensates for impaired neural function generated by defects in several signalling pathways, including PI3K in SH-SY5Y-E cells. Our expression profiling data will be useful for further elucidation of the signal transduction-transcriptional network involving PI3K or TRKB.

Acquisition and reduction of data obtained from tank 101-SY in-situ ball rheometer

International Nuclear Information System (INIS)

Shepard, C.L.; Chieda, M.A.; Kirihara, L.J.; Phillips, J.R.; Shekarriz, A.; Terrones, G.; Abbott, J.; Unal, C.; Pasamehmetoglu, K.O.; Graham, A.

1995-02-01

Development of the ball rheometer to measure rheological properties and density of the waste in Hanford Tank 241-SY-101 will be completed around September 1994. This instrument is expected to provide the first-of-its-kind in-situ measurements of the fluid properties of the waste contained within this tank. A mixer pump has been installed in this tank, and this pump has been very successful at mitigating the flammable gas problem associated with Tank 101-SY. The ball rheometer will serve as a diagnostic tool for judging the effectiveness of mixing in Tank 101-SY and others and will be one of few in-situ probes available for diagnostic measurements. Based on experiments performed at Los Alamos National Laboratory and Pacific Northwest Laboratory, it is believed that a generalized Bingham fluid model (Herschel-Bulkley fluid model) may be useful for describing at least some of the waste contained in Tank 101-SY, and data obtained in the tank will initially be reduced using this fluid model. The single largest uncertainty in the determination of the drag force on the ball is the drag force which will be experienced by the cable attached to the ball. This drag can be a substantial fraction of the total drag when the ball is deep within the tank. It is expected that the fluid properties may be history dependent, thus rheological properties of the undisturbed fluid may be different from the same properties after the fluid has been disturbed by passage of the ball. The data collection strategy allows the determination of the waste fluid rheology both in the undisturbed state and after it has been disturbed by the ball. Unlike the rheological parameters, measurement of density requires no model for its interpretation; however, the effects of yield stress may need to be accounted for. This measurement can be made with fairly good accuracy and may provide the most useful data in determination of mixer pump effectiveness

Work plan for transition of SY-101 hydrogen mitigation test project data acquisition and control system (DACS-1)

International Nuclear Information System (INIS)

McClees, J.; Truitt, R.W.

1994-01-01

The purpose of this effort is to transfer operating and maintenance responsibility for the 241-SY-101 data acquisition and control system (DACS-1) from Los Alamos National Laboratory to Westinghouse Hanford Company. This work plan defines the tasks required for a successful turnover. It identifies DACS-1 transition, deliverables, responsible organizations and individuals, interfaces, cost, and schedule. The transition plan will discuss all required hardware, software, documentation, maintenance, operations, and training for use at Hanford Waste Tank 241-SY-101. The transfer of responsibilities for DACS-1 to WHC is contingent on final approval of applicable Acceptance for Beneficial Use documentation by Waste Tank Operations. The DACS-1 was designed to provide data monitoring, display, and storage for Tank 241-SY-101. The DACS-1 also provides alarm and control of all the hydrogen mitigation testing systems, as well as ancillary systems and equipment (HVAC, UPS, etc.) required to achieve safe and reliable operation of the testing systems in the tank

Caspase Activation of p21-Activated Kinase 2 Occurs During Cisplatin-Induced Apoptosis of SH-SY5Y Neuroblastoma Cells and in SH-SY5Y Cell Culture Models of Alzheimer’s and Parkinson’s Disease

Directory of Open Access Journals (Sweden)

Jerry W. Marlin

2010-04-01

Full Text Available p21-activated kinase 2 (PAK-2 appears to have a dual function in the regulation of cell survival and cell death. Activation of full-length PAK-2 by the p21 G-proteins Rac or Cdc42 stimulates cell survival. However, PAK-2 is unique among the PAK family because it is also activated through proteolytic cleavage by caspase 3 or similar caspases to generate the constitutively active PAK-2p34 fragment. Caspase activation of PAK-2 correlates with the induction of apoptosis in response to many stimuli and recombinant expression of PAK-2p34 has been shown to stimulate apoptosis in several human cell lines. Here, we show that caspase activation of PAK-2 also occurs during cisplatin-induced apoptosis of SH-SY5Y neuroblastoma cells as well as in SH-SY5Y cell culture models for Alzheimer’s and Parkinson’s disease. Inhibition of mitochondrial complex I or of ubiquitin/proteasome-mediated protein degradation, which both appear to be involved in Parkinson’s disease, induce apoptosis and caspase activation of PAK-2 in SH-SY5Y cells. Overexpression of the amyloid precursor protein, which results in accumulation and aggregation of β-amyloid peptide, the main component of β-amyloid plaques in Alzheimer’s disease, also induces apoptosis and caspase activation of PAK-2 in SH-SY5Y cells. Expression of the PAK-2 regulatory domain inhibits caspase-activated PAK-2p34 and prevents apoptosis in 293T human embryonic kidney cells, indicating that caspase activation of PAK-2 is directly involved in the apoptotic response. This is the first evidence that caspase activation of PAK-2 correlates with apoptosis in cell culture models of Alzheimer’s and Parkinson’s disease and that selective inhibition of caspase-activated PAK-2p34 could prevent apoptosis.

A discussion on the safety classification of the tank 241-SY-101 mixer pump

International Nuclear Information System (INIS)

Van Vleet, R.J.

1997-01-01

An analysis, consistent with the methodology used in the draft TWRS FSAR (HNF-SD-WM-SAR-067), is presented to show that the classification of the mixer pump in tank 241-SY-101 should be safety significant

The effect of dilution on the gas-retention behavior of Tank 241-SY-101 waste

International Nuclear Information System (INIS)

Bredt, P.R.; Tingey, S.M.; Shade, E.H.

1995-09-01

The effect of dilution on gas retention in waste from Tank 241-SY-101 was investigated. A composite sample was prepared from material collected during the Window ''C'' and Window ''E'' sampling events. The composite contained material from both the convective and nonconvective layer in the proportions existing in the tank. Operation of the mixer pump in Tank 241-SY-101 has homogenized the tank material, and dilution of the current waste would require additional mixing; therefore, no attempt was made to use unhomogenized tank waste to prepare the composite. The composite was diluted with 2 M NaOH at ratios of 0.5:1, 0.75: 1, 1:1, and 3:1 per volume (2 M NaOH:tank waste)

Acetaldehyde Induces Cytotoxicity of SH-SY5Y Cells via Inhibition of Akt Activation and Induction of Oxidative Stress

Directory of Open Access Journals (Sweden)

Tingting Yan

2016-01-01

Full Text Available Excessive alcohol consumption can lead to brain tissue damage and cognitive dysfunction. It has been shown that heavy drinking is associated with an earlier onset of neurodegenerative diseases such as Alzheimer’s disease. Acetaldehyde, the most toxic metabolite of ethanol, is speculated to mediate the brain tissue damage and cognitive dysfunction induced by the chronic excessive consumption of alcohol. However, the exact mechanisms by which acetaldehyde induces neurotoxicity are not totally understood. In this study, we investigated the cytotoxic effects of acetaldehyde in SH-SY5Y cells and found that acetaldehyde induced apoptosis of SH-SY5Y cells by downregulating the expression of antiapoptotic Bcl-2 and Bcl-xL and upregulating the expression of proapoptotic Bax. Acetaldehyde treatment led to a significant decrease in the levels of activated Akt and cyclic AMP-responsive element binding protein (CREB. In addition, acetaldehyde induced the activation of p38 mitogen-activated protein kinase (MAPK while inhibiting the activation of extracellular signal-regulated kinases (ERKs, p44/p42MAPK. Meanwhile, acetaldehyde treatment caused an increase in the production of reactive oxygen species and elevated the oxidative stress in SH-SY5Y cells. Therefore, acetaldehyde induces cytotoxicity of SH-SY5Y cells via promotion of apoptotic signaling, inhibition of cell survival pathway, and induction of oxidative stress.

A Multidimensional Model of School Dropout from an 8-Year Longitudinal Study in a General High School Population

Science.gov (United States)

Fortin, Laurier; Marcotte, Diane; Diallo, Thierno; Potvin, Pierre; Royer, Egide

2013-01-01

This study tests an empirical multidimensional model of school dropout, using data collected in the first year of an 8-year longitudinal study, with first year high school students aged 12-13 years. Structural equation modeling analyses show that five personal, family, and school latent factors together contribute to school dropout identified at…

Design basis and requirements for 241-SY Modular Exhauster concrete pad and retaining wall

International Nuclear Information System (INIS)

Kriskovich, J.R.

1994-01-01

The purpose of this document is to serve as the design and functional requirements for a concrete pad for the new 241-SY Modular Exhauster and for a retaining wall to be built near the new ventilation systems

Engineer/constructor description of work for Tank 241-SY-102 retrieval system, project W-211, initial tank retrieval systems

International Nuclear Information System (INIS)

Rieck, C.A.

1996-02-01

This document provides a description of work for the design and construction of a waste retrieval system for Tank 241-SY-102. The description of work includes a working estimate and schedule, as well as a narrative description and sketches of the waste retrieval system. The working estimate and schedule are within the established baselines for the Tank 241-SY-102 retrieval system. The technical baseline is provided in Functional Design Criteria, WHC-SD-W211-FDC-001, Revision 2

Baicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism.

Science.gov (United States)

Song, Ju-Xian; Choi, Mandy Yuen-Man; Wong, Kavin Chun-Kit; Chung, Winkie Wing-Yan; Sze, Stephen Cho-Wing; Ng, Tzi-Bun; Zhang, Kalin Yan-Bo

2012-01-21

Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism. Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-binding probe Hoechst 33258. The production of reactive oxidative species (ROS) and loss of mitochondrial membrane potential (ΔΨm) were determined by fluorescent staining with DCFH-DA and Rhodanmine 123, respectively. The expression of Bax, Bcl-2, cleaved caspase-3 and phosphorylated ERK1/2 was determined by the Western blots. Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner. Pre- and subsequent co-treatment with baicalein preserved the cell morphology and attenuated the nuclear apoptotic characteristics triggered by rotenone. Baicalein antagonized rotenone-induced overproduction of ROS, loss of ΔΨm, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2. The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.

CompTIA security+ review guide exam SY0-401

CERN Document Server

Stewart , James M

2014-01-01

Focused review for the CompTIA Security+ certification exam The CompTIA Security+ certification offers tremendous opportunities for IT professionals. For those who want to take their careers to the next level, CompTIA Security+ Review Guide: Exam SY0-401 is here to serve as a great resource for certification preparation. This concise, focused guide is easy to use and is organized by each exam objective for quick review and reinforcement of key topics. You'll find information on network security, compliance and operational security, and threats and vulnerabilities. Additionally, this indispensa

241-SY-101 air lance removal lessons learned

International Nuclear Information System (INIS)

Moore, T.L.; Titzler, P.A.

1994-01-01

An emergency task was undertaken to remove four air lances and one thermocouple (TC) tree from tank 241-SY-101 (SY-101). This resulted from video observation that these pipes were being severely bent during periodic gas release events that regularly occurred every three to four months. At the time, the gas release events were considered to be the number one safety issue within the US Department of Energy (DOE) complex. This emergency removal task was undertaken on an extremely short schedule that required all activities possible to be completed in parallel. This approach and extremely short schedule, while successful, resulted in some undesirable consequences from less than desired time for design, reviews, equipment testing, operations training, and bad weather conditions. These consequences included leakage of liquid waste from the containers to the ground, higher than expected dose rates at the container surface, difficult field operations, and unexpected pipe configuration during removal. In addition, changes to environmental regulations and severe winter weather impacted the packaging and shipping activities required the prepare the removed pipes for storage at the Central Waste Complex (CWC). The purpose of this document is to identify lessons to be learned for future activities. In context of the emergency conditions that existed at the time and the urgency to remove these pipes, their removal was successfully completed under extremely difficult conditions and schedule. The success of the task should not be overshadowed by the desire to identify areas needing improvement and lessons to be learned. Many of the lessons identified in this document have already resulted in improved conduct of operations and engineering

In Shop Acceptance Test Report for the SY Farm Annulus Leak Detectors

International Nuclear Information System (INIS)

SMITH, S.G.

1999-01-01

The following test report was written for the SY tank farm annulus leak detectors. The test plan used was HNF-4546, Revision 1. The purpose of the test plan was to test the ENRAF series 854 ATG with SPU II card prior to installation. The test plan set various parameters and verifies the gauge and alarms functionality

PROF. ULRICH GERHARD LAUTS (1787— 1865). In sy studie „De ...

African Journals Online (AJOL)

Test

In sy studie „De beteekenis van 1813 voor Nederlands geestelijke beschaving"') het die .... af te lê. Daarna het hulle hul eed nog weer bevestig om trou te bly aan ..... L. 'n ver dedigende skrywe aan die Minister gestuur na aanleiding van 'n twis met die militêre geneesheer („Rijksarchief," Dept. van Marine. Prof. U. G..

Dibutyryl cyclic AMP induces differentiation of human neuroblastoma SH-SY5Y cells into a noradrenergic phenotype.

Science.gov (United States)

Kume, Toshiaki; Kawato, Yuka; Osakada, Fumitaka; Izumi, Yasuhiko; Katsuki, Hiroshi; Nakagawa, Takayuki; Kaneko, Shuji; Niidome, Tetsuhiro; Takada-Takatori, Yuki; Akaike, Akinori

2008-10-10

Dibutyryl cyclic AMP (dbcAMP) and retinoic acid (RA) have been demonstrated to be the inducers of morphological differentiation in SH-SY5Y cells, a human catecholaminergic neuroblastoma cell line. However, it remains unclear whether morphologically differentiated SH-SY5Y cells by these compounds acquire catecholaminergic properties. We focused on the alteration of tyrosine hydroxylase (TH) expression and intracellular content of noradrenaline (NA) as the indicators of functional differentiation. Three days treatment with dbcAMP (1mM) and RA (10microM) induced morphological changes and an increase of TH-positive cells using immunocytochemical analysis in SH-SY5Y cells. The percentage of TH-expressing cells in dbcAMP (1mM) treatment was larger than that in RA (10microM) treatment. In addition, dbcAMP increased intracellular NA content, whereas RA did not. The dbcAMP-induced increase in TH-expressing cells is partially inhibited by KT5720, a protein kinase A (PKA) inhibitor. We also investigated the effect of butyrate on SH-SY5Y cells, because dbcAMP is enzymatically degraded by intracellular esterase, thereby resulting in the formation of butyrate. Butyrate induced the increase of NA content at lower concentrations than dbcAMP, although the increase in TH-expressing cells by butyrate was smaller than that by dbcAMP. The dbcAMP (1mM)- and butyrate (0.3mM)-induced increase in NA content was completely suppressed by alpha-methyl-p-tyrosine (1mM), an inhibitor of TH. These results suggest that dbcAMP induces differentiation into the noradrenergic phenotype through both PKA activation and butyrate.

Curcumin Attenuated Bupivacaine-Induced Neurotoxicity in SH-SY5Y Cells Via Activation of the Akt Signaling Pathway.

Science.gov (United States)

Fan, You-Ling; Li, Heng-Chang; Zhao, Wei; Peng, Hui-Hua; Huang, Fang; Jiang, Wei-Hang; Xu, Shi-Yuan

2016-09-01

Bupivacaine is widely used for regional anesthesia, spinal anesthesia, and pain management. However, bupivacaine could cause neuronal injury. Curcumin, a low molecular weight polyphenol, has a variety of bioactivities and may exert neuroprotective effects against damage induced by some stimuli. In the present study, we tested whether curcumin could attenuate bupivacaine-induced neurotoxicity in SH-SY5Y cells. Cell injury was evaluated by examining cell viability, mitochondrial damage and apoptosis. We also investigated the levels of activation of the Akt signaling pathway and the effect of Akt inhibition by triciribine on cell injury following bupivacaine and curcumin treatment. Our findings showed that the bupivacaine treatment could induce neurotoxicity. Pretreatment of the SH-SY5Y cells with curcumin significantly attenuated bupivacaine-induced neurotoxicity. Interestingly, the curcumin treatment increased the levels of Akt phosphorylation. More significantly, the pharmacological inhibition of Akt abolished the cytoprotective effect of curcumin against bupivacaine-induced cell injury. Our data suggest that pretreating SH-SY5Y cells with curcumin provides a protective effect on bupivacaine-induced neuronal injury via activation of the Akt signaling pathway.

Computer system requirements specification for 101-SY hydrogen mitigation test project data acquisition and control system (DACS-1)

International Nuclear Information System (INIS)

McNeece, S.G.; Truitt, R.W.

1994-01-01

The system requirements specification for SY-101 hydrogen mitigation test project (HMTP) data acquisition and control system (DACS-1) documents the system requirements for the DACS-1 project. The purpose of the DACS is to provide data acquisition and control capabilities for the hydrogen mitigation testing of Tank SY-101. Mitigation testing uses a pump immersed in the waste, directed at varying angles and operated at different speeds and time durations. Tank and supporting instrumentation is brought into the DACS to monitor the status of the tank and to provide information on the effectiveness of the mitigation test. Instrumentation is also provided for closed loop control of the pump operation. DACS is also capable for being expanded to control and monitor other mitigation testing. The intended audience for the computer system requirements specification includes the SY-101 hydrogen mitigation test data acquisition and control system designers: analysts, programmers, instrument engineers, operators, maintainers. It is intended for the data users: tank farm operations, mitigation test engineers, the Test Review Group (TRG), data management support staff, data analysis, Hanford data stewards, and external reviewers

Soil structure interaction analysis for the Hanford Site 241-SY-101 double-shell waste storage tanks

International Nuclear Information System (INIS)

Giller, R.A.; Weiner, E.O.

1991-09-01

The 241-SY-101 tank is a double-shell waste storage tank buried in the 241-SY tank farm in the 200 West Area of the Hanford Site. This analysis addresses the effects of seismic soil-structure interaction on the tank structure and includes a parametric soil-structure interaction study addressing three configurations: two-dimensional soil structure, a two-dimensional structure-soil-structure, and a three-dimensional soil-structure interaction. This study was designed to determine an optimal method for addressing seismic-soil effects on underground storage tanks. The computer programs calculate seismic-soil pressures on the double-shell tank walls and and seismic acceleration response spectra in the tank. The results of this soil-structure interaction parametric study as produced by the computer programs are given in terms of seismic soil pressures and response spectra. The conclusions of this soil-structure interaction evaluation are that dynamically calculated soil pressures in the 241-SY-101 tank are significantly reduce from those using standard hand calculation methods and that seismic evaluation of underground double-shell waste storage tanks must consider soil-structure interaction effects in order to predict conservative structural response. Appendixes supporting this study are available in Volume 2 of this report

Comparing Slovenian year 8 and year 9 elementary school pupils’ knowledge of electrolyte chemistry and their intrinsic motivation

OpenAIRE

Devetak, Iztok; Drofenik Lorber, Erna; Juriševič, Mojca; Glažar, Saša

2015-01-01

This study explored the differences between eight-year elementary school pupils (before the curriculum reform) and nine-year elementary school pupils (soon after the curriculum reform) in Slovenia, as regards specific chemistry knowledge and motivation to learn chemistry. Altogether, 191 elementary school pupils participated in the study. The results show that pupils of nine-year elementary school are not significantly better at chemistry knowledge test scores than eight-year elementary schoo...

UV time-dependent emission in SY Muscae

International Nuclear Information System (INIS)

Michalitsianos, A.G.; Kafatos, M.

1982-01-01

Ultraviolet spectra acquired with the International Ultraviolet Explorer (IUE) of SY Mus = HD 10036 on 20 September 1980 and 11 June 1981 indicate a substantial enhancement of UV emission over a nine month period. The general UV flux level appears to have increased by approximately one order of magnitude between the first and second observing epochs. The strong ultraviolet continuum evident throughout the entire IUE spectral range lambdalambda1200-3200 A on 11 June 1981 is closely approximated by a star with Tsub(eff) = 40,000 K, where previously on 20 September 1980 the continuum distribution presented a more complex structure that is possibly explained by a combination of thermal emission from an early type main sequence star, and nebular recombination emission (Michalitsianos et al. 1981). (Auth.)

Neurotoxicity of "ecstasy" and its metabolites in human dopaminergic differentiated SH-SY5Y cells.

Science.gov (United States)

Ferreira, Patrícia Silva; Nogueira, Tiago Bernandes; Costa, Vera Marisa; Branco, Paula Sério; Ferreira, Luísa Maria; Fernandes, Eduarda; Bastos, Maria Lourdes; Meisel, Andreas; Carvalho, Félix; Capela, João Paulo

2013-02-04

"Ecstasy" (3,4-methylenedioxymethamphetamine or MDMA) is a widely abused recreational drug, reported to produce neurotoxic effects, both in laboratory animals and in humans. MDMA metabolites can be major contributors for MDMA neurotoxicity. This work studied the neurotoxicity of MDMA and its catechol metabolites, α-methyldopamine (α-MeDA) and N-methyl-α-methyldopamine (N-Me-α-MeDA) in human dopaminergic SH-SY5Y cells differentiated with retinoic acid and 12-O-tetradecanoyl-phorbol-13-acetate. Differentiation led to SH-SY5Y neurons with higher ability to accumulate dopamine and higher resistance towards dopamine neurotoxicity. MDMA catechol metabolites were neurotoxic to SH-SY5Y neurons, leading to caspase 3-independent cell death in a concentration- and time-dependent manner. MDMA did not show a concentration- and time-dependent death. Pre-treatment with the antioxidant and glutathione precursor, N-acetylcysteine (NAC), resulted in strong protection against the MDMA metabolites' neurotoxicity. Neither the superoxide radical scavenger, tiron, nor the inhibitor of the dopamine (DA) transporter, GBR 12909, prevented the metabolites' toxicity. Cells exposed to α-MeDA showed an increase in intracellular glutathione (GSH) levels, which, at the 48 h time-point, was not dependent in the activity increase of γ-glutamylcysteine synthetase (γ-GCS), revealing a possible transient effect. Importantly, pre-treatment with buthionine sulfoximine (BSO), an inhibitor of γ-GCS, prevented α-MeDA induced increase in GSH levels, but did not augment this metabolite cytotoxicity. Even so, BSO pre-treatment abolished NAC protective effects against α-MeDA neurotoxicity, which were, at least partially, due to GSH de novo synthesis. Inversely, pre-treatment of cells with BSO augmented N-Me-α-MeDA-induced neurotoxicity, but only slightly affected NAC neuroprotection. In conclusion, MDMA catechol metabolites promote differential toxic effects to differentiated dopaminergic human SH-SY

One-step transvaginal three-dimensional hysterosalpingo-foam sonography (3D-HyFoSy) confirmation test for Essure® follow-up: a multicenter study.

Science.gov (United States)

Zizolfi, B; Lazzeri, L; Franchini, M; Di Spiezio Sardo, A; Nappi, C; Piccione, E; Exacoustos, C

2018-01-01

To evaluate, in patients who underwent Fallopian-tube sterilization by hysteroscopic insertion of an Essure® device, the feasibility and accuracy of three-dimensional (3D) transvaginal sonography (TVS) to check the position of the device and 3D hysterosalpingo-foam sonography (3D-HyFoSy) using contrast-enhanced gel foam to assess consequent tubal occlusion. This was a prospective multicenter study conducted from June 2012 to July 2014 in four Italian centers, which included 50 women who underwent hysteroscopic Essure microinsert placement in a total of 95 Fallopian tubes. Placement of the microinserts was performed in an outpatient setting following standard procedure recommendations. All patients underwent transvaginal 3D-HyFoSy and hysterosalpingography (HSG) approximately 12-14 weeks after the procedure. The position of the devices was first checked on 3D-TVS and classified according to specific criteria (Positions A, B, C and D). Then, 3D-HyFoSy with ExEm® gel foam as the ultrasound contrast agent was performed to confirm tubal occlusion by the absence of contrast agent within the tubes and/or around the ovaries. To evaluate the feasibility of 3D-HyFoSy, consecutive volume acquisitions were performed while injecting the gel foam. After sonographic evaluation, women underwent HSG to assess the success of sterilization, as standard methodology. Side effects and pain evoked during and after 3D-HyFoSy and HSG were evaluated using a numeric pain rating scale. On 3D-TVS, 10 devices (10.5%) were in Position A, two (2.1%) in Position B, 59 (62.1%) in Position C and 24 (25.3%) in Position D. During 3D-HyFoSy, tubal occlusion was observed in 89 of 95 tubes (93.7%). In the six cases in which the passage of the contrast was observed, one device (16.7%) was in Position B, one device (16.7%) in Position D and four devices (66.7%) were found to lie in Position C. Tubal patency was confirmed at HSG with a concordance rate of 100%. The mean pain score associated with 3D-HyFoSy

Epidemiology of school accidents during a six school-year period in one region in Poland.

Science.gov (United States)

Sosnowska, Stefania; Kostka, Tomasz

2003-01-01

The aim of the study was to analyse the incidence of school accidents in relation to school size, urban/rural environment and conditions of physical education classes. 202 primary schools with nearly 50,000 students aged 7-15 years were studied during a 6-year period in the Włocławek region in Poland. There were in total 3274 school accidents per 293,000 student-years. Accidents during breaks (36.6%) and physical education (33.2%) were most common. Most frequently accidents took place at schoolyard (29.7%), gymnasium (20.2%), and in the corridor and stairs (25.2%). After adjustment for students' age and sex, student-staff ratio and duration of school hours, urban environment increased the probability of accident (OR: 1.25; 95% CI: 1.14-1.38). Middle-size schools (8-23 classes) had similar accident rate as small schools (OR: 0.93; 95% CI: 0.83-1.04), while schools with 24-32 classes (OR: 1.26; 95% CI: 1.10-1.43) and with > or = 33 classes (OR: 1.36; 95% CI: 1.17-1.58) had increased accident rate. Presence of a gymnasium was also associated with increased probability of accident (OR: 1.49; 95% CI: 1.38-1.61). Urban environment, larger school-size and equipment with full-size gymnasium are important and independent risk factors for school accidents. These findings provide some new insights into the epidemiology of school-related accidents and may be useful information for the planning of strategies to reduce accident incidence in schools.

Neurotoxicity Induced by Bupivacaine via T-Type Calcium Channels in SH-SY5Y Cells

Science.gov (United States)

Wen, Xianjie; Xu, Shiyuan; Liu, Hongzhen; Zhang, Quinguo; Liang, Hua; Yang, Chenxiang; Wang, Hanbing

2013-01-01

There is concern regarding neurotoxicity induced by the use of local anesthetics. A previous study showed that an overload of intracellular calcium is involved in the neurotoxic effect of some anesthetics. T-type calcium channels, which lower the threshold of action potentials, can regulate the influx of calcium ions. We hypothesized that T-type calcium channels are involved in bupivacaine-induced neurotoxicity. In this study, we first investigated the effects of different concentrations of bupivacaine on SH-SY5Y cell viability, and established a cell injury model with 1 mM bupivacaine. The cell viability of SH-SY5Y cells was measured following treatment with 1 mM bupivacaine and/or different dosages (10, 50, or 100 µM) of NNC 55-0396 dihydrochloride, an antagonist of T-type calcium channels for 24 h. In addition, we monitored the release of lactate dehydrogenase, cytosolic Ca2+ ([Ca2+]i), cell apoptosis and caspase-3 expression. SH-SY5Y cells pretreated with different dosages (10, 50, or 100 µM) of NNC 55-0396 dihydrochloride improved cell viability, reduced lactate dehydrogenase release, inhibited apoptosis, and reduced caspase-3 expression following bupivacaine exposure. However, the protective effect of NNC 55-0396 dihydrochloride plateaued. Overall, our results suggest that T-type calcium channels may be involved in bupivacaine neurotoxicity. However, identification of the specific subtype of T calcium channels involved requires further investigation. PMID:23658789

Baicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism

Directory of Open Access Journals (Sweden)

Song Ju-Xian

2012-01-01

Full Text Available Abstract Background Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism. Methods Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-binding probe Hoechst 33258. The production of reactive oxidative species (ROS and loss of mitochondrial membrane potential (ΔΨm were determined by fluorescent staining with DCFH-DA and Rhodanmine 123, respectively. The expression of Bax, Bcl-2, cleaved caspase-3 and phosphorylated ERK1/2 was determined by the Western blots. Results Baicalein significantly increased viability and decreased rotenone-induced death of SH-SY5Y cells in a dose-dependent manner. Pre- and subsequent co-treatment with baicalein preserved the cell morphology and attenuated the nuclear apoptotic characteristics triggered by rotenone. Baicalein antagonized rotenone-induced overproduction of ROS, loss of ΔΨm, the increased expression of Bax, cleaved caspase-3 and phosphorylated ERK1/2 and the decreased expression of Bcl-2. Conclusion The antioxidative effect, mitochondrial protection and modulation of anti-and pro-apoptotic proteins are related to the neuroprotective effects of baicalein against rotenone induced cell death in SH-SY5Y cells.

Neurotoxicity induced by bupivacaine via T-type calcium channels in SH-SY5Y cells.

Directory of Open Access Journals (Sweden)

Xianjie Wen

Full Text Available There is concern regarding neurotoxicity induced by the use of local anesthetics. A previous study showed that an overload of intracellular calcium is involved in the neurotoxic effect of some anesthetics. T-type calcium channels, which lower the threshold of action potentials, can regulate the influx of calcium ions. We hypothesized that T-type calcium channels are involved in bupivacaine-induced neurotoxicity. In this study, we first investigated the effects of different concentrations of bupivacaine on SH-SY5Y cell viability, and established a cell injury model with 1 mM bupivacaine. The cell viability of SH-SY5Y cells was measured following treatment with 1 mM bupivacaine and/or different dosages (10, 50, or 100 µM of NNC 55-0396 dihydrochloride, an antagonist of T-type calcium channels for 24 h. In addition, we monitored the release of lactate dehydrogenase, cytosolic Ca(2+ ([Ca2+]i, cell apoptosis and caspase-3 expression. SH-SY5Y cells pretreated with different dosages (10, 50, or 100 µM of NNC 55-0396 dihydrochloride improved cell viability, reduced lactate dehydrogenase release, inhibited apoptosis, and reduced caspase-3 expression following bupivacaine exposure. However, the protective effect of NNC 55-0396 dihydrochloride plateaued. Overall, our results suggest that T-type calcium channels may be involved in bupivacaine neurotoxicity. However, identification of the specific subtype of T calcium channels involved requires further investigation.

Examination of physical activity in adolescents over the school year.

Science.gov (United States)

Bruner, Mark W; Chad, Karen E; Beattie-Flath, Jodie A; Humbert, M Louise; Verrall, Tanya C; Vu, Lan; Muhajarine, Nazeem

2009-11-01

This study monitored the physical activity behavior of adolescent students over a ten month school year. Physical activity was assessed at two month intervals using self-report and objective (Actical accelerometers) measures. Self-report results (n = 547) indicated a decline in physical activity throughout the school year for all grades and genders. The decline was attributed largely to a decrease in organized activity participation. Objective physical activity results (n = 40) revealed a significant decline in activity in the latter half of the school year (February to June). Declining physical activity was attributed to a decrease in vigorous activity which was consistent across grade and gender. Collectively, the results highlight the importance of promoting consistent opportunities for adolescents to be active throughout the school year.

Safety evaluation for adding water to tank 101-SY

International Nuclear Information System (INIS)

Clinton, R.

1994-01-01

This document provides a new water limit for Tank 241-SY-101. The original limit was set at 9600 gallons. The new limit is now 20,000 gallons. There are various activities that require the use of additional water to the tank. The main activity is the removal of the temporary mixer pump. This requires a large amount of water which will exceed the original limit. Also, other activities such as flushing, adding a viscometer, and adding a void fraction meter requires additional water. The new limit safely incorporates these activities and allows room for more future activities

Safety equipment list for the 241-SY-101 RAPID mitigation project

Energy Technology Data Exchange (ETDEWEB)

MORRIS, K.L.

1999-06-29

This document provides the safety classification for the safety (safety class and safety RAPID Mitigation Project. This document is being issued as the project SEL until the supporting authorization basis documentation, this document will be superseded by the TWRS SEL (LMHC 1999), documentation istlralized. Upon implementation of the authorization basis significant) structures, systems, and components (SSCS) associated with the 241-SY-1O1 which will be updated to include the information contained herein.

Safety equipment list for the 241-SY-101 RAPID mitigation project

International Nuclear Information System (INIS)

Morris, K.L.

1999-01-01

This document provides the safety classification for the safety (safety class and safety RAPID Mitigation Project. This document is being issued as the project SEL until the supporting authorization basis documentation, this document will be superseded by the TWRS SEL (LMHC 1999), documentation istlralized. Upon implementation of the authorization basis significant) structures, systems, and components (SSCS) associated with the 241-SY-1O1 which will be updated to include the information contained herein

Sports-related injuries among high school athletes--United States, 2005-06 school year.

Science.gov (United States)

2006-09-29

Participation in high school sports helps promote a physically active lifestyle. High school sports participation has grown from an estimated 4 million participants during the 1971-72 school year to an estimated 7.2 million in 2005-06. However, despite the documented health benefits of increased physical activity (e.g., weight management, improved self-esteem, and increased strength, endurance, and flexibility), those who participate in athletics are at risk for sports-related injuries. High school athletes account for an estimated 2 million injuries, 500,000 doctor visits, and 30,000 hospitalizations annually. To date, the study of these injuries has been limited by inabilities to calculate injury rates, compare results among groups, and generalize findings from small, nonrepresentative samples. During the 2005-06 school year, researchers at a children's hospital in Ohio used an Internet-based data-collection tool to pilot an injury surveillance system among athletes from a representative national sample of U.S. high schools. This report summarizes the findings of that study, which indicated that participation in high school sports resulted in an estimated 1.4 million injuries at a rate of 2.4 injuries per 1,000 athlete exposures (i.e., practices or competitions). Surveillance of exposure-based injury rates in a nationally representative sample of high school athletes and analysis of injury patterns can help guide activities aimed at reducing these injuries.

Palmitic acid induces neurotoxicity and gliatoxicity in SH-SY5Y human neuroblastoma and T98G human glioblastoma cells

Directory of Open Access Journals (Sweden)

Yee-Wen Ng

2018-04-01

Full Text Available Background Obesity-related central nervous system (CNS pathologies like neuroinflammation and reactive gliosis are associated with high-fat diet (HFD related elevation of saturated fatty acids like palmitic acid (PA in neurons and astrocytes of the brain. Methods Human neuroblastoma cells SH-SY5Y (as a neuronal model and human glioblastoma cells T98G (as an astrocytic model, were treated with 100–500 µM PA, oleic acid (OA or lauric acid (LA for 24 h or 48 h, and their cell viability was assessed by 3-(4,5-dimetylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay. The effects of stable overexpression of γ-synuclein (γ-syn, a neuronal protein recently recognized as a novel regulator of lipid handling in adipocytes, and transient overexpression of Parkinson’s disease (PD α-synuclein [α-syn; wild-type (wt and its pathogenic mutants A53T, A30P and E46K] in SH-SY5Y and T98G cells, were also evaluated. The effects of co-treatment of PA with paraquat (PQ, a Parkinsonian pesticide, and leptin, a hormone involved in the brain-adipose axis, were also assessed. Cell death mode and cell cycle were analyzed by Annexin V/PI flow cytometry. Reactive oxygen species (ROS level was determined using 2′,7′-dichlorofluorescien diacetate (DCFH-DA assay and lipid peroxidation level was determined using thiobarbituric acid reactive substances (TBARS assay. Results MTT assay revealed dose- and time-dependent PA cytotoxicity on SH-SY5Y and T98G cells, but not OA and LA. The cytotoxicity was significantly lower in SH-SY5Y-γ-syn cells, while transient overexpression of wt α-syn or its PD mutants (A30P and E46K, but not A53T modestly (but still significantly rescued the cytotoxicity of PA in SH-SY5Y and T98G cells. Co-treatment of increasing concentrations of PQ exacerbated PA’s neurotoxicity. Pre-treatment of leptin, an anti-apoptotic adipokine, did not successfully rescue SH-SY5Y cells from PA-induced cytotoxicity—suggesting a mechanism of PA

Structural analysis of multiport riser 5A installation on tank 241SY101

Energy Technology Data Exchange (ETDEWEB)

Strehlow, J.P.

1994-09-16

The Tank 101-SY multiport riser assembly in the 241-SY-101 waste tank will replace the existing 42 inch riser with four smaller ports. Each smaller port can be used independently to access the tank interior with equipment and instruments needed to mitigate the concentration of hydrogen in the tank. This document provides a design report on the structural evaluation of the multiport riser assembly as well as its anchorage. The multiport riser assembly is a steel structure installed directly above the 42-inch riser and sealed at the existing riser flange. The assembly is structurally supported by the concrete pad placed around the 42 inch riser. The multiport riser assembly will provide two 8-inch penetrations, one 12-inch penetration and one 24-inch penetration. Each penetration will have a shielding plate. These penetrations will be used to insert equipment such as a sonic probe into the tank. In addition to normal loads, non-reactor Safety Class 1 structures, systems and components are to withstand the effects of extreme environmental loads including Design Basis Earthquake (DBE), Design Basis Wind (DBW), Design Basis Flood, Volcanic Eruptions and other abnormal loads considered on a case by case basis. Non-reactor Safety Class 2, 3 and 4 structures, systems and components are those that are not Safety Class 1 and are respectively specified as onsite safety related, occupational safety related and non-safety related items. The 241-SY-101 tank is considered as a non-reactor Safety Class 1 structure. The multiport riser assembly is considered as a non-reactor Safety Class 2 structure since it serves to contain the radioactive and toxic materials under normal operating conditions. However, the pressure relief doors provided on the assembly are considered as Safety Class 1 structures.

Progress toward mitigation of flammable gas Tank 241-SY-101

International Nuclear Information System (INIS)

Lentsch, J.W.; Babad, H.; Hanson, C.E.; Kirch, N.W.

1994-01-01

The mixing pump installed in Hanford Site tank 241-SY-101 has been shown to be effective in releasing flammable gases in a controlled manner. This controlled release of gas prevents the accumulation and episodic release above flammable limits. More work needs to be done to optimize the pumping operation, and to evaluate the long-term effects of mixing so as to assure that no undesirable changes have occurred to the waste. Other alternative mitigation concepts are still being evaluated as a backup to mixing

Modulation of chemotherapy-induced cytotoxicity in SH-SY5Y neuroblastoma cells by caffeine and chlorogenic acid.

Science.gov (United States)

Hall, Susan; Anoopkumar-Dukie, Shailendra; Grant, Gary D; Desbrow, Ben; Lai, Richard; Arora, Devinder; Hong, Yinna

2017-06-01

Chemotherapy is an important treatment modality for malignancy but is limited by significant toxicity and it susceptibility to numerous drug interactions. While the interacting effects with medications are well known, there is limited evidence on the interaction with commonly consumed food and natural products. The aim of this study was to evaluate the bioactive constituents of coffee (caffeine and chlorogenic acid) on the cytotoxicity of doxorubicin, gemcitabine, and paclitaxel in vitro. Pretreatment with caffeine (100 nM and 10 μM) sensitized SH-SY5Y cells to doxorubicin-induced toxicity and increased apoptosis and sensitized PC3 cells to gemcitabine-induced toxicity. Pretreatment with 10 μM caffeine decreased total cell reactive oxygen species (ROS) production but increased mitochondrial ROS production. In contrast, caffeine (10 nM and 10 μM) protected cells against gemcitabine-induced toxicity and apoptosis. Similarly, 1 μM and 10 μM caffeine protected cells against paclitaxel-induced toxicity and mitochondrial ROS production. Chlorogenic acid had no effect on chemotherapy-induced toxicity in SH-SY5Y cells. In conclusion, this study provides preliminary evidence that caffeine, not chlorogenic acid, modulates the cytotoxicity of doxorubicin, gemcitabine, and paclitaxel in SH-SY5Y cells via different mechanisms.

Analysis of the Catecholaminergic Phenotype in Human SH-SY5Y and BE(2-M17 Neuroblastoma Cell Lines upon Differentiation.

Directory of Open Access Journals (Sweden)

Roberta Filograna

Full Text Available Human cell lines are often used to investigate cellular pathways relevant for physiological or pathological processes or to evaluate cell toxicity or protection induced by different compounds, including potential drugs. In this study, we analyzed and compared the differentiating activities of three agents (retinoic acid, staurosporine and 12-O-tetradecanoylphorbol-13-acetate on the human neuroblastoma SH-SY5Y and BE(2-M17 cell lines; the first cell line is largely used in the field of neuroscience, while the second is still poorly characterized. After evaluating their effects in terms of cell proliferation and morphology, we investigated their catecholaminergic properties by assessing the expression profiles of the major genes involved in catecholamine synthesis and storage and the cellular concentrations of the neurotransmitters dopamine and noradrenaline. Our results demonstrate that the two cell lines possess similar abilities to differentiate and acquire a neuron-like morphology. The most evident effects in SH-SY5Y cells were observed in the presence of staurosporine, while in BE(2-M17 cells, retinoic acid induced the strongest effects. Undifferentiated SH-SY5Y and BE(2-M17 cells are characterized by the production of both NA and DA, but their levels are considerably higher in BE(2-M17 cells. Moreover, the NAergic phenotype appears to be more pronounced in SH-SY5Y cells, while BE(2-M17 cells have a more prominent DAergic phenotype. Finally, the catecholamine concentration strongly increases upon differentiation induced by staurosporine in both cell lines. In conclusion, in this work the catecholaminergic phenotype of the human BE(2-M17 cell line upon differentiation was characterized for the first time. Our data suggest that SH-SY5Y and BE(2-M17 represent two alternative cell models for the neuroscience field.

Analysis of the Catecholaminergic Phenotype in Human SH-SY5Y and BE(2)-M17 Neuroblastoma Cell Lines upon Differentiation.

Science.gov (United States)

Filograna, Roberta; Civiero, Laura; Ferrari, Vanni; Codolo, Gaia; Greggio, Elisa; Bubacco, Luigi; Beltramini, Mariano; Bisaglia, Marco

2015-01-01

Human cell lines are often used to investigate cellular pathways relevant for physiological or pathological processes or to evaluate cell toxicity or protection induced by different compounds, including potential drugs. In this study, we analyzed and compared the differentiating activities of three agents (retinoic acid, staurosporine and 12-O-tetradecanoylphorbol-13-acetate) on the human neuroblastoma SH-SY5Y and BE(2)-M17 cell lines; the first cell line is largely used in the field of neuroscience, while the second is still poorly characterized. After evaluating their effects in terms of cell proliferation and morphology, we investigated their catecholaminergic properties by assessing the expression profiles of the major genes involved in catecholamine synthesis and storage and the cellular concentrations of the neurotransmitters dopamine and noradrenaline. Our results demonstrate that the two cell lines possess similar abilities to differentiate and acquire a neuron-like morphology. The most evident effects in SH-SY5Y cells were observed in the presence of staurosporine, while in BE(2)-M17 cells, retinoic acid induced the strongest effects. Undifferentiated SH-SY5Y and BE(2)-M17 cells are characterized by the production of both NA and DA, but their levels are considerably higher in BE(2)-M17 cells. Moreover, the NAergic phenotype appears to be more pronounced in SH-SY5Y cells, while BE(2)-M17 cells have a more prominent DAergic phenotype. Finally, the catecholamine concentration strongly increases upon differentiation induced by staurosporine in both cell lines. In conclusion, in this work the catecholaminergic phenotype of the human BE(2)-M17 cell line upon differentiation was characterized for the first time. Our data suggest that SH-SY5Y and BE(2)-M17 represent two alternative cell models for the neuroscience field.

Tank 241-SY-101 surface level rise remediation test and evaluation plan for transfer system

International Nuclear Information System (INIS)

BAUER, R.E.

1999-01-01

The purpose of this testing and evaluation plan (TEP) is to provide the high level guidance on testing requirements for ensuring that the equipment and systems to be implemented for remediation of the SY-101 waste level rise USQ are effective

Human neuroblastoma SH-SY5Y cells show increased resistance to hyperthermic stress after differentiation, associated with elevated levels of Hsp72.

Science.gov (United States)

Cheng, Lesley; Smith, Danielle J; Anderson, Robin L; Nagley, Phillip

2011-01-01

Terminally differentiated neurones in the central nervous system need to be protected from stress. We ask here whether differentiation of progenitor cells to neurones is accompanied by up-regulation of Hsp72, with acquisition of enhanced thermotolerance. Human neuroblastoma SH-SY5Y cells were propagated in an undifferentiated form and subsequently differentiated into neurone-like cells. Thermotolerance tests were carried out by exposure of cells to various temperatures, monitoring nuclear morphology as index of cell death. Abundance of Hsp72 was measured in cell lysates by western immunoblotting. The differentiation of SH-SY5Y cells was accompanied by increased expression of Hsp72. Further, in both cell states, exposure to mild hyperthermic stress (43°C for 30 min) increased Hsp72 expression. After differentiation, SH-SY5Y cells were more resistant to hyperthermic stress compared to their undifferentiated state, correlating with levels of Hsp72. Stable exogenous expression of Hsp72 in SH-SY5Y cells (transfected line 5YHSP72.1, containing mildly elevated levels of Hsp72), led to enhanced resistance to hyperthermic stress. Hsp72 was found to be inducible in undifferentiated 5YHSP72.1 cells; such heat-treated cells displayed enhanced thermotolerance. Treatment of cells with KNK437, a suppressor of Hsp72 induction, resulted in acute thermosensitisation of all cell types tested here. Hsp72 has a major role in the enhanced hyperthermic resistance acquired during neuronal differentiation of SH-SY5Y cells. These findings model the requirement in intact organisms for highly differentiated neurones to be specially protected against thermal stress.

Early Years Swimming: A Way of Supporting School Transitions?

Science.gov (United States)

Jorgensen, Robyn

2016-01-01

Informal learning contexts may provide opportunities for adding capital to young children in their years prior to schooling. This paper explores the potential of the early-years swim context to add capital to young children that may position them favourably for the transition to school. Using Bourdieu's notion of capital, the paper discusses the…

Safety Evaluation for Packaging 101-SY Hydrogen Mitigation Mixer Pump package

International Nuclear Information System (INIS)

Carlstrom, R.F.

1994-01-01

This Safety Evaluation for Packaging (SEP) provides analysis and considered necessary to approve a one-time transfer of the 101-SY Hydrogen Mitigation Mixer Pump (HMMP). This SEP will demonstrate that the transfer of the HMMP in a new shipping container will provide an equivalent degree of safety as would be provided by packages meeting US Department of Transportation (DOT)/US Nuclear Regulatory Commission (NRC) requirements. This fulfills onsite, transportation requirements implemented by WHC-CM-2-14

Safety Evaluation for Packaging 101-SY Hydrogen Mitigation Mixer Pump package

Energy Technology Data Exchange (ETDEWEB)

Carlstrom, R.F.

1994-10-05

This Safety Evaluation for Packaging (SEP) provides analysis and considered necessary to approve a one-time transfer of the 101-SY Hydrogen Mitigation Mixer Pump (HMMP). This SEP will demonstrate that the transfer of the HMMP in a new shipping container will provide an equivalent degree of safety as would be provided by packages meeting US Department of Transportation (DOT)/US Nuclear Regulatory Commission (NRC) requirements. This fulfills onsite, transportation requirements implemented by WHC-CM-2-14.

Moving House for Education in the Pre-School Years

Science.gov (United States)

Hansen, Kirstine

2014-01-01

This paper uses data from the Millennium Cohort Study (MCS) to examine house moves that take place in the pre-school years, focusing on families who move for the education of their children. We present results showing that education- related house moves do indeed occur in the pre-school years with particular types of parents making these…

Semisynthesis of SY-1 for investigation of breast cancer stem cell selectivity of C-ring-modified salinomycin analogues.

Science.gov (United States)

Huang, Xiaoli; Borgström, Björn; Månsson, Linda; Persson, Lo; Oredsson, Stina; Hegardt, Cecilia; Strand, Daniel

2014-07-18

Salinomycin, a naturally occurring polyether ionophore was recently found to selectively reduce the proportion of CD44(+)/CD24(-) cells, a phenotype associated with breast cancer stem cells. Subsequent studies from our group showed that chemical modification of the allylic C20 hydroxyl of salinomycin, located at the C-ring, can enhance the activity of derivatives against breast cancer cells over 5-fold compared to the native structure. Access to C-ring-modified salinomycin analogues is thus of interest from both a mechanistic and a synthetic perspective. Here, we report efficient strategies for gram scale synthesis of the natural product SY-1 (20-deoxy salinomycin), and a saturated analogue, 18,19-dihydro SY-1, for a comparative in vitro investigation of the biological profiles of these compounds with that of salinomycin. Across several assays, the deoxygenated structures required higher concentrations to elicit similar cellular responses to that of salinomycin. Similarly to salinomycin, SY-1 or 18,19-dihydro SY-1 treatment was found to reduce the proportion of CD44(+)/CD24(-) cells with essentially complete selectivity up to ∼IC25. Importantly, the proportion of CD44(+)/CD24(-) cells showed a pronounced U-shaped dose response curve for salinomycin and its derivatives, but not for paclitaxel. The concentration for maximum response in this assay followed differences in IC50 for salinomycin and its analogues, which emphasizes the importance of taking concentration dependence into account when comparing effects on the CD44(+)/CD24(-) phenotype. Small differences in the global conformation within the triad of compounds investigated together with differences in activity across assays emphasize the importance of substitution at C20 for the activity of salinomycin and its derivatives.

System design description for SY-101 hydrogen mitigation test project data acquisition and control system (DACS-1)

International Nuclear Information System (INIS)

Truitt, R.W.; Pounds, T.S.; Smith, S.O.

1994-01-01

This document describes the hardware subsystems of the data acquisition and control system (DACS) used in mitigation tests conducted on waste tank SY-101 at the Hanford Nuclear Reservation. The system was designed and implemented by Los Alamos National Laboratory (LANL) and supplied to Westinghouse Hanford Company (WHC). The mitigation testing uses a pump immersed in the waste tank, directed at certain angles and operated at different speeds and time durations. The SY-101 tank has experienced recurrent periodic gas releases of hydrogen, nitrous oxide, ammonia, and (recently discovered) methane. The hydrogen gas represents a danger, as some of the releases are in amounts above the lower flammability limit (LFL). These large gas releases must be mitigated. Several instruments have been added to the tank to monitor the gas compositions, the tank level, the tank temperature, and other parameters. A mixer pump has been developed to stir the tank waste to cause the gases to be released at a slow rate. It is the function of the DACS to monitor those instruments and to control the mixer pump in a safe manner. During FY93 and FY94 the mixer pump was installed with associated testing operations support equipment and a mitigation test project plan was implemented. These activities successfully demonstrated the mixer pump's ability to mitigate the SY-101 tank hydrogen gas hazard

[Resistance of Lactobacillus casei subsp. casei SY13 and Lactobacillus delbrueckii subsp. bulgaricus LJJ to reactive oxygen species].

Science.gov (United States)

Zhang, Shuwen; Lv, Jiaping; Menghe, Bilige; Zhang, Heping; Zhang, Liyu; Song, Jinhui; Wang, Zhifei

2009-02-01

We evaluated antioxidative effect of two antioxidative strains, isolated from the traditional fermented dairy products. Both intact cells and cell-free extract of Lactobacillus casei subsp. casei SY13 and Lactobacillus delbrueckii subsp. bulgaricus LJJ were used to study the inhibited effect of linoleic acid peroxidation, the ability of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide anion radical,the ability of tolerancing hydrogen peroxide and the chelating capacity of ferrous ion and reducting activity. Lactobacillus casei subsp. casei SY13 and Lactobacillus delbrueckii subsp. bulgaricus LJJ demonstrated highest inhibition on linoleic acid peroxidation by 62.95% and 66.16%, respectively. The cell-free extract showed excellent scavenging superoxide anion and hydroxyl radicals activity. However, the intact cells of Lactobacillus delbrueckii subsp. bulgaricus LJJ scavenging superoxide and hydroxyl radicals capacity were not detected. The intact cells of Lactobacillus casei subsp. casei SY13 and Lactobacillus delbrueckii subsp. bulgaricus LJJ on 1,1-diphenyl-2-picrylhydrazyl radical scavenging ability and chelating ferrous ion capacity were superior to cell-free extract. The highest reduced activety was equivalent to 305 micromol/L and 294 micromol/L L-cysteine. Two latobacilli strains had good antioxidant capacity. As potential probiotics, it can be used in future.

Acceptance test report, 241-SY-101 Flexible Receiver System, Phase 3 testing

International Nuclear Information System (INIS)

Ritter, G.A.

1995-01-01

This document summarizes the results of the phase 3 acceptance test of the 241-SY-101 Flexible Receiver System (FRS). The purpose of this acceptance test is to verify the sealing integrity of the FRS to ensure that the release of waste and aerosols will be minimized during the removal of the test mixer pump from Tank 241-SY-101. The FRS is one of six major components of the Equipment Removal System, which has been designed to retrieve, transport, and store the mixer pump. This acceptance test was performed at the 306E Facility in the 300 area from January 10, 1995 to January 17, 1995. The Phase 3 test consisted of two parts. Part one was a water leak test of the seal between the blast shield and mock load distribution frame (LDF) to ensure that significant contamination of the pump pit and waste interaction with the aluminum impact-limiting material under the LDF are prevented during the pump removal operation. The second part of this acceptance test was an air leak test of the assembled flexible receiver system. The purpose of this test was to verify that the release of hazardous aerosols will be minimized if the tank dome pressure becomes slightly positive during the decontamination of the mixer pump

In situ determination of rheological properties and void fraction in Hanford Waste Tank 241-SY-101

International Nuclear Information System (INIS)

Stewart, C.W.; Shepard, C.L.; Alzheimer, J.M.; Stokes, T.I.; Terrones, G.

1995-08-01

This report presents the results of the operation of the void fraction instrument (VFI) and ball rheometer in Hanford Tank 241-SY-101, which contains approximately one million gallons of radioactive waste. These instruments provided the first direct assay of the waste condition in the tank after more than a year of mixer pump operation. The two instruments were deployed in the tank in late 1994 and early 1995 to gather much-needed data on the effect prolonged mixer pump operation has on gas retention in the waste. The information supplied by these instruments has filled a great gap in the quantitative knowledge of the waste condition. The results show that the solids are well-mixed by the current mixer pump to within less than a meter of the tank bottom. Undisturbed sludge remains only on the lowest 10--30 cm and contains 10--12% void. The mixed slurry above contains less than 1% void and has no measurable yield strength and a shear-thinning viscosity of approximately 6 Poise at 1 sec -1 . Estimating the gas volumes in each of the four layers based on VFI data yields a total of 221 ± 57 m 3 (7,800 ± 2,000 SCF) of gas at 1 atmosphere. Given the current waste level of 10.2 m (400 inches), the degassed waste level would be 9.8 m (386 inches). These results confirm that the mixer pump in Tank 241-SY-101 has performed the job it was installed to do--thoroughly mix the waste to release stored gas and prevent gas accumulation

Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells

Science.gov (United States)

Li, Le; Zhang, Qing-guo; Lai, Lu-ying; Wen, Xian-jie; Zheng, Ting; Cheung, Chi-wai; Zhou, Shu-qin; Xu, Shi-yuan

2013-01-01

Local anesthetics are used routinely and effectively. However, many are also known to activate neurotoxic pathways. We tested the neuroprotective efficacy of ginkgolide B (GB), an active component of Ginkgo biloba, against ROS-mediated neurotoxicity caused by the local anesthetic bupivacaine. SH-SY5Y cells were treated with different concentrations of bupivacaine alone or following preincubation with GB. Pretreatment with GB increased SH-SY5Y cell viability and attenuated intracellular ROS accumulation, apoptosis, mitochondrial dysfunction, and ER stress. GB suppressed bupivacaine-induced mitochondrial depolarization and mitochondria complex I and III inhibition and increased cleaved caspase-3 and Htra2 expression, which was strongly indicative of activation of mitochondria-dependent apoptosis with concomitantly enhanced expressions of Grp78, caspase-12 mRNA, protein, and ER stress. GB also improved ultrastructural changes indicative of mitochondrial and ER damage induced by bupivacaine. These results implicate bupivacaine-induced ROS-dependent mitochondria, ER dysfunction, and apoptosis, which can be attenuated by GB through its antioxidant property. PMID:24228138

Composition, preparation, and gas generation results from simulated wastes of Tank 241-SY-101

International Nuclear Information System (INIS)

Bryan, S.A.; Pederson, L.R.

1994-08-01

This document reviews the preparation and composition of simulants that have been developed to mimic the wastes temporarily stored in Tank 241-SY-101 at Hanford. The kinetics and stoichiometry of gases that are generated using these simulants are also compared, considering the roles of hydroxide, chloride, and transition metal ions; the identities of organic constituents; and the effects of dilution, radiation, and temperature. Work described in this report was conducted for the Flammable Gas Safety Program at Pacific Northwest Laboratory, (a) whose purpose is to develop information that is necessary to mitigate potential safety hazards associated with waste tanks at the Hanford Site. The goal of this research and of related efforts at the Georgia Institute of Technology (GIT), Argonne National Laboratory (ANL), and Westinghouse Hanford Company (WHC) is to determine the thermal and thermal/radiolytic mechanisms by which flammable and other gases are produced in Hanford wastes, emphasizing those stored in Tank 241-SY-101. A variety of Tank 241-SY-101 simulants have been developed to date. The use of simulants in laboratory testing activities provides a number of advantages, including elimination of radiological risks to researchers, lower costs associated with experimentation, and the ability to systematically alter simulant compositions to study the chemical mechanisms of reactions responsible for gas generation. The earliest simulants contained the principal inorganic components of the actual waste and generally a single complexant such as N-(2-hydroxyethyl) ethylenediaminetriacetic acid (HEDTA) or ethylenediaminetriacetic acid (EDTA). Both homogeneous and heterogeneous compositional forms were developed. Aggressive core sampling and analysis activities conducted during Windows C and E provided information that was used to design new simulants that more accurately reflected major and minor inorganic components

Ultrasonic Examination of Double-Shell Tank 241-SY-103. Examination completed February 2004

International Nuclear Information System (INIS)

Pardini, Allan F.; Posakony, Gerald J.

2004-01-01

COGEMA Engineering Corporation (COGEMA), under a contract from CH2M Hill Hanford Group (CH2M Hill), has performed an ultrasonic nondestructive examination of selected portions of Double-Shell Tank 241-SY-103. The purpose of this examination was to provide information that could be used to evaluate the integrity of the wall of the primary tank. The requirements for the ultrasonic examination of Tank 241-SY-103 were to detect, characterize (identify, size, and locate), and record measurements made of any wall thinning, pitting, or cracks that might be present in the wall of the primary tank. Any measurements that exceed the requirements set forth in the Engineering Task Plan (ETP), RPP-17750 (Jensen 2003) and summarized on page 1 of this document, are reported to CH2M Hill and the Pacific Northwest National Laboratory (PNNL) for further evaluation. Under the contract with CH2M Hill, all data is to be recorded on disk and paper copies of all measurements are provided to PNNL for third-party evaluation. PNNL is responsible for preparing a report that describes the results of the COGEMA ultrasonic examinations

Ultrasonic Examination of Double-Shell Tank 241-SY-102. Examination Completed June 2004

International Nuclear Information System (INIS)

Pardini, Allan F.; Posakony, Gerald J.

2004-01-01

COGEMA Engineering Corporation (COGEMA), under a contract from CH2M Hill Hanford Group (CH2M Hill), has performed an ultrasonic nondestructive examination of selected portions of Double-Shell Tank 241-SY-102. The purpose of this examination was to provide information that could be used to evaluate the integrity of the wall of the primary tank. The requirements for the ultrasonic examination of Tank 241-SY-102 were to detect, characterize (identify, size, and locate), and record measurements made of any wall thinning, pitting, or cracks that might be present in the wall of the primary tank. Any measurements that exceed the requirements set forth in the Engineering Task Plan (ETP), RPP-17750 (Jensen 2003) and/SUMmarized on page 1 of this document, are reported to CH2M Hill and the Pacific Northwest National Laboratory (PNNL) for further evaluation. Under the contract with CH2M Hill, all data is to be recorded on disk and paper copies of all measurements are provided to PNNL for third-party evaluation. PNNL is responsible for preparing a report that describes the results of the COGEMA

Sharing data between mobile devices, connected vehicles, and infrastructure task 3: system requirements specifications (SyRS) final.

Science.gov (United States)

2016-07-14

This report describes the system requirements specifications (SyRS) for the use of mobile devices in a connected vehicle environment. Specifically, it defines the different types of requirements (functional, interface, performance, security, data, an...

French in Lesotho schools forty years after independence ...

African Journals Online (AJOL)

Most independent African states are now, like Lesotho, about forty years old. What has become of foreign languages such as French that once thrived under colonial rule albeit mostly in schools targeting non-indigenous learners? In Lesotho French seems to be the preserve of private or “international” schools. Can African ...

Differentiated Human SH-SY5Y Cells Provide a Reductionist Model of Herpes Simplex Virus 1 Neurotropism.

Science.gov (United States)

Shipley, Mackenzie M; Mangold, Colleen A; Kuny, Chad V; Szpara, Moriah L

2017-12-01

Neuron-virus interactions that occur during herpes simplex virus (HSV) infection are not fully understood. Neurons are the site of lifelong latency and are a crucial target for long-term suppressive therapy or viral clearance. A reproducible neuronal model of human origin would facilitate studies of HSV and other neurotropic viruses. Current neuronal models in the herpesvirus field vary widely and have caveats, including incomplete differentiation, nonhuman origins, or the use of dividing cells that have neuropotential but lack neuronal morphology. In this study, we used a robust approach to differentiate human SH-SY5Y neuroblastoma cells over 2.5 weeks, producing a uniform population of mature human neuronal cells. We demonstrate that terminally differentiated SH-SY5Y cells have neuronal morphology and express proteins with subcellular localization indicative of mature neurons. These neuronal cells are able to support a productive HSV-1 infection, with kinetics and overall titers similar to those seen in undifferentiated SH-SY5Y cells and the related SK-N-SH cell line. However, terminally differentiated, neuronal SH-SY5Y cells release significantly less extracellular HSV-1 by 24 h postinfection (hpi), suggesting a unique neuronal response to viral infection. With this model, we are able to distinguish differences in neuronal spread between two strains of HSV-1. We also show expression of the antiviral protein cyclic GMP-AMP synthase (cGAS) in neuronal SH-SY5Y cells, which is the first demonstration of the presence of this protein in nonepithelial cells. These data provide a model for studying neuron-virus interactions at the single-cell level as well as via bulk biochemistry and will be advantageous for the study of neurotropic viruses in vitro IMPORTANCE Herpes simplex virus (HSV) affects millions of people worldwide, causing painful oral and genital lesions, in addition to a multitude of more severe symptoms such as eye disease, neonatal infection, and, in rare

78 FR 55121 - Submission for Review: Self-Certification of Full-Time School Attendance for the School Year, RI...

Science.gov (United States)

2013-09-09

... Attendance for the School Year, RI 25-14 and Information; and Instructions for Completing the Self...-0032, Self-Certification of Full-Time School Attendance For The School Year, RI 25-14; and Information... technological collection techniques or other forms of information technology, e.g., permitting electronic...

Measuring Temporal Resolution (Release of Masking) with a Hughson-Westlake Up-Down Instead of a Békèsy-Tracking Procedure.

Science.gov (United States)

Rhebergen, Koenraad S; van Esch, Thamar E M; Dreschler, Wouter A

2015-06-01

A temporal resolution test in addition to the pure-tone audiogram may be of great clinical interest because of its relevance in speech perception and expected relevance in hearing aid fitting. Larsby and Arlinger developed an appropriate clinical test, but this test uses a Békèsy-tracking procedure for estimating masked thresholds in stationary and interrupted noise to assess release of masking (RoM) for temporal resolution. Generally the Hughson-Westlake up-down procedure is used in the clinic to measure the pure-tone thresholds in quiet. A uniform approach will facilitate clinical application and might be appropriate for RoM measurements as well. Because there is no golden standard for measuring the RoM in the clinic, we examine in the present study the Hughson-Westlake up-down procedure to measure the RoM and compare the results with the Békèsy-tracking procedure. The purpose of the current study was to examine the differences between a Békèsy-tracking procedure and the Hughson-Westlake up-down procedure for estimating masked thresholds in stationary and interrupted noise to assess RoM. RoM is assessed in eight normal-hearing (NH) and ten hearing-impaired (HI) listeners through both methods. Results from both methods are compared with each other and with predicted thresholds from a model. Wilcoxon signed-rank tests, paired t tests. Some differences between the two methods were found. We used a model to quantify the results of the two measurement procedures. The results of the Hughson-Westlake procedure were clearly better in agreement with the model than the results of the Békèsy-tracking procedure. Furthermore, the Békèsy-tracking procedure showed more spread in the results of the NH listeners than the Hughson-Westlake procedure. The Hughson-Westlake procedure seems to be an applicable alternative for measuring RoM for temporal resolution in the clinical audiological practice. American Academy of Audiology.

CompTIA Security+ Deluxe Study Guide Exam SY0-301

CERN Document Server

Dulaney, Emmett

2011-01-01

Get a host of extras with this Deluxe version including a Security Administration Simulator!  Prepare for CompTIA's new Security+ exam SY0-301 with this Deluxe Edition of our popular CompTIA Security+ Study Guide, 5th Edition. In addition to the 100% coverage of all exam essentials and study tools you'll find in the regular study guide, the Deluxe Edition gives you over additional hands-on lab exercises and study tools, three additional practice exams, author videos, and the exclusive Security Administration simulator. This book is a CompTIA Recommended product. Provides 100% coverage of all e

Emulsion-core and polyelectrolyte-shell nanocapsules: biocompatibility and neuroprotection against SH-SY5Y cells

International Nuclear Information System (INIS)

Piotrowski, Marek; Szczepanowicz, Krzysztof; Jantas, Danuta; Leśkiewicz, Monika; Lasoń, Władysław; Warszyński, Piotr

2013-01-01

The emulsion-core and polyelectrolyte-coated nanocapsules, designed as water-insoluble neuroprotective drug delivery system, were synthesized using layer-by-layer saturation method. The isopropyl myristate was used as oil phase and docusate sodium salt as emulsifier. For the polyelectrolyte shell preparation, synthetic polyelectrolytes, cationic (PDADMAC, PAH, and PLL) and anionic (PGA) were used. The particle size and zeta potential of nanocapsules were characterized by the dynamic light scattering. The average size of synthesized nanocapsules ranged from ∼80 to ∼100 nm. Zeta potential values ranged from less than approximately −30 mV for the polyanion layers to greater than approximately +30 mV for the polycation layers. Biocompatibilities of the synthesized nanocarriers were evaluated against SH-SY5Y human neuroblastoma cells using various biochemical assays. The results obtained show that synthesized nanocapsules coated with PLL and PGA were nontoxic to SH-SY5Y cells, and they were used as nanocarriers for model neuroprotective drug (a calpain inhibitor MDL 28170). The neuroprotective action of the encapsulated MDL 28170 against hydrogen peroxide-induced oxidative stress cytotoxicity was evaluated in the same cell line. The results showed that nanoencapsulated form of MDL 28170 were biocompatible and protected SH-SY5Y cells against the H 2 O 2 (0.5 mM/24 h)-induced damage in 20–40 times lower concentrations than those of the same drug added directly to the culture medium. These data suggest that the nanoscale carriers of neuroprotective drugs might serve as novel promising therapeutic agents for oxidative stress-related neurodegenerative processes

Emulsion-core and polyelectrolyte-shell nanocapsules: biocompatibility and neuroprotection against SH-SY5Y cells

Energy Technology Data Exchange (ETDEWEB)

Piotrowski, Marek, E-mail: ncpiotro@cyf-kr.edu.pl; Szczepanowicz, Krzysztof [Polish Academy of Sciences, Jerzy Haber Institute of Catalysis and Surface Chemistry (Poland); Jantas, Danuta; Leśkiewicz, Monika; Lasoń, Władysław [Polish Academy of Sciences, Institute of Pharmacology (Poland); Warszyński, Piotr [Polish Academy of Sciences, Jerzy Haber Institute of Catalysis and Surface Chemistry (Poland)

2013-11-15

The emulsion-core and polyelectrolyte-coated nanocapsules, designed as water-insoluble neuroprotective drug delivery system, were synthesized using layer-by-layer saturation method. The isopropyl myristate was used as oil phase and docusate sodium salt as emulsifier. For the polyelectrolyte shell preparation, synthetic polyelectrolytes, cationic (PDADMAC, PAH, and PLL) and anionic (PGA) were used. The particle size and zeta potential of nanocapsules were characterized by the dynamic light scattering. The average size of synthesized nanocapsules ranged from ∼80 to ∼100 nm. Zeta potential values ranged from less than approximately −30 mV for the polyanion layers to greater than approximately +30 mV for the polycation layers. Biocompatibilities of the synthesized nanocarriers were evaluated against SH-SY5Y human neuroblastoma cells using various biochemical assays. The results obtained show that synthesized nanocapsules coated with PLL and PGA were nontoxic to SH-SY5Y cells, and they were used as nanocarriers for model neuroprotective drug (a calpain inhibitor MDL 28170). The neuroprotective action of the encapsulated MDL 28170 against hydrogen peroxide-induced oxidative stress cytotoxicity was evaluated in the same cell line. The results showed that nanoencapsulated form of MDL 28170 were biocompatible and protected SH-SY5Y cells against the H{sub 2}O{sub 2} (0.5 mM/24 h)-induced damage in 20–40 times lower concentrations than those of the same drug added directly to the culture medium. These data suggest that the nanoscale carriers of neuroprotective drugs might serve as novel promising therapeutic agents for oxidative stress-related neurodegenerative processes.

Oral cleanliness of 12-13-year-old and 15-year-old school children of Sunsari District, Nepal

Directory of Open Access Journals (Sweden)

Yee R

2006-09-01

Full Text Available The aim of the study was to evaluate the oral cleanliness of school children in the District of Sunsari, Nepal. A multi-stage random sampling oral epidemiological survey was conducted in private and government, urban, rural town and rural village schools in 15 illakas of Sunsari District, Eastern Nepal. A total of 600, 12-13-year-old and 600 15-year-old school children were examined by trained examiners using the simplified oral hygiene index (OHI-S. The average age-group, debris and calculus index scores were combined to obtain the simplified oral hygiene index (OHI-S. The mean OHI-S scores were compared and evaluated using the parametric t-test for two independent samples. The mean OHI-S for urban 12-13-year-old school children was 0.98 compared to 1.34 for school children of rural towns and 1.44 for school children of rural villages and these differences in mean OHI-S were statistically significant ( P < 0.005. In the 15-year-old age group, urban school children had a mean OHI-S score of 1.00 compared to 1.37 for rural towns and 1.43 for rural villages. The variance in the mean OHI-S scores were statistically significant ( P < 0.005. The overall level of cleanliness in the school children surveyed was good. Children of urban schools had the lowest scores followed by school children from rural towns and then rural villages. When the mean OHI-S scores were compared with the DMFT scores, there was an inverse relationship between oral cleanliness and dental caries. Frequency of sugar consumption and the availability and affordability of fluoridated toothpaste may be important factors in the development of dental caries than oral cleanliness.

Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP+

OpenAIRE

Khwanraj, Kawinthra; Phruksaniyom, Chareerut; Madlah, Suriyat; Dharmasaroja, Permphan

2015-01-01

The human neuroblastoma SH-SY5Y cell line has been used as a dopaminergic cell model for Parkinson's disease research. Whether undifferentiated or differentiated SH-SY5Y cells are more suitable remains controversial. This study aims to evaluate the expression of apoptosis-related mRNAs activated by MPP+ and evaluate the differential expression of tyrosine hydroxylase (TH) in undifferentiated and retinoic acid- (RA-) induced differentiated cells. The western blot results showed a gradual decre...

Overexpression of tissue-nonspecific alkaline phosphatase increases the expression of neurogenic differentiation markers in the human SH-SY5Y neuroblastoma cell line.

Science.gov (United States)

Graser, Stephanie; Mentrup, Birgit; Schneider, Doris; Klein-Hitpass, Ludger; Jakob, Franz; Hofmann, Christine

2015-10-01

Patients suffering from the rare hereditary disease hypophosphatasia (HPP), which is based on mutations in the ALPL gene, tend to develop central nervous system (CNS) related issues like epileptic seizures and neuropsychiatric illnesses such as anxiety and depression, in addition to well-known problems with the mineralization of bones and teeth. Analyses of the molecular role of tissue-nonspecific alkaline phosphatase (TNAP) in transgenic SH-SY5Y(TNAPhigh) neuroblastoma cells compared to SH-SY5Y(TNAPlow) cells indicate that the enzyme influences the expression levels of neuronal marker genes like RNA-binding protein, fox-1 homolog 3 (NEUN) and enolase 2, gamma neuronal (NSE) as well as microtubule-binding proteins like microtubule-associated protein 2 (MAP2) and microtubule-associated protein tau (TAU) during neurogenic differentiation. Fluorescence staining of SH-SY5Y(TNAPhigh) cells reveals TNAP localization throughout the whole length of the developed projection network and even synapsin Ι co-localization with strong TNAP signals at some spots at least at the early time points of differentiation. Additional immunocytochemical staining shows higher MAP2 expression in SH-SY5Y(TNAPhigh) cells and further a distinct up-regulation of tau and MAP2 in the course of neurogenic differentiation. Interestingly, transgenic SH-SY5Y(TNAPhigh) cells are able to develop longer cellular processes compared to control cells after stimulation with all-trans retinoic acid (RA). Current therapies for HPP prioritize improvement of the bone phenotype. Unraveling the molecular role of TNAP in extraosseous tissues, like in the CNS, will help to improve treatment strategies for HPP patients. Taking this rare disease as a model may also help to dissect TNAP's role in neurodegenerative diseases and even improve future treatment of common pathologies. Copyright © 2015 Elsevier Inc. All rights reserved.

Pregnancy outcome after hystero-salpingo-contrast-sonography (HyCoSy versus hysterosalpingography (HSG using different contrast media

Directory of Open Access Journals (Sweden)

Emad M. Siam

2011-12-01

Conclusion: The pregnancy rates after performing HyCoSy using the proposed homogenously mixed saline and water are nearly the same as those following HSG using different contrast media with inherent advantage of avoiding exposure to radiation.

Preparing for the Flu During the 2009-10 School Year: Questions and Answers for Schools

Science.gov (United States)

US Department of Education, 2009

2009-01-01

This brochure provides answers to the following questions: (1) Why do school districts, schools, teachers, parents, and communities need to plan for the continuation of learning for students during flu season this year? (2) How should districts and schools go about planning to continue students' education when they are at home because of H1N1?…

The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells

OpenAIRE

Ana O. Pires; Andreia Neves-Carvalho; Nuno Sousa; António J. Salgado

2014-01-01

The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y). For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above. Retinoic ...

Analysis of the 241SY101 pump removal trailer and the 241SY101 strongback

International Nuclear Information System (INIS)

Coverdell, B.L.

1995-09-01

The purpose of the calculations contained in the attached appendix is to determine the vibrational stability of the following combination (The Combination); shipping container, strongback and trailer. The vibrational stability of The Combination will be determined with the shipping container and strongback in the upright position. If the natural frequency of The Combination coincides with the input frequency and no damping is present, resonance will occur. The result of this is that the natural frequency of the Combination must be calculated as well as the input frequency. The input frequency in this case is caused by wind. Due to their geometrical complexity the upper and lower hydraulic clevises were analyzed for structural adequacy by using finite-element analysis (FEA). The FEA software COSMOS/M version 1.70 was used to model the upper and lower hydraulic clevis. All designs are in accordance with Standard Architectural-Civil Design Criteria, Design Loads for Facilities (DOE-RL 1989) and are safety class 3. The design and fabrication of each component is in accordance with American Institute of Steel Construction, Manual of Steel Construction, (AISC, 1989). The analyses contained in this document reflects the as-built condition of the 241SY101 hydraulic trailer

Probabilistic safety assessment for Hanford high-level waste tank 241-SY-101

Energy Technology Data Exchange (ETDEWEB)

MacFarlane, D.R.; Bott, T.F.; Brown, L.F.; Stack, D.W. [Los Alamos National Lab., NM (United States); Kindinger, J.; Deremer, R.K.; Medhekar, S.R.; Mikschl, T.J. [PLG, Inc., Newport Beach, CA (United States)

1994-05-01

Los Alamos National Laboratory (Los Alamos) is performing a comprehensive probabilistic safety assessment (PSA), which will include consideration of external events for the 18 tank farms at the Hanford Site. This effort is sponsored by the Department of Energy (DOE/EM, EM-36). Even though the methodology described herein will be applied to the entire tank farm, this report focuses only on the risk from the weapons-production wastes stored in tank number 241-SY-101, commonly known as Tank 101-SY, as configured in December 1992. This tank, which periodically releases ({open_quotes}burps{close_quotes}) a gaseous mixture of hydrogen, nitrous oxide, ammonia, and nitrogen, was analyzed first because of public safety concerns associated with the potential for release of radioactive tank contents should this gas mixture be ignited during one of the burps. In an effort to mitigate the burping phenomenon, an experiment is being conducted in which a large pump has been inserted into the tank to determine if pump-induced circulation of the tank contents will promote a slow, controlled release of the gases. At the Hanford Site there are 177 underground tanks in 18 separate tank farms containing accumulated liquid/sludge/salt cake radioactive wastes from 50 yr of weapons materials production activities. The total waste volume is about 60 million gal., which contains approximately 120 million Ci of radioactivity.

Probabilistic safety assessment for Hanford high-level waste tank 241-SY-101

International Nuclear Information System (INIS)

MacFarlane, D.R.; Bott, T.F.; Brown, L.F.; Stack, D.W.; Kindinger, J.; Deremer, R.K.; Medhekar, S.R.; Mikschl, T.J.

1994-05-01

Los Alamos National Laboratory (Los Alamos) is performing a comprehensive probabilistic safety assessment (PSA), which will include consideration of external events for the 18 tank farms at the Hanford Site. This effort is sponsored by the Department of Energy (DOE/EM, EM-36). Even though the methodology described herein will be applied to the entire tank farm, this report focuses only on the risk from the weapons-production wastes stored in tank number 241-SY-101, commonly known as Tank 101-SY, as configured in December 1992. This tank, which periodically releases (open-quotes burpsclose quotes) a gaseous mixture of hydrogen, nitrous oxide, ammonia, and nitrogen, was analyzed first because of public safety concerns associated with the potential for release of radioactive tank contents should this gas mixture be ignited during one of the burps. In an effort to mitigate the burping phenomenon, an experiment is being conducted in which a large pump has been inserted into the tank to determine if pump-induced circulation of the tank contents will promote a slow, controlled release of the gases. At the Hanford Site there are 177 underground tanks in 18 separate tank farms containing accumulated liquid/sludge/salt cake radioactive wastes from 50 yr of weapons materials production activities. The total waste volume is about 60 million gal., which contains approximately 120 million Ci of radioactivity

Acceptance test report, 241-SY-101 Flexible Receiver System, Phase 1 testing

International Nuclear Information System (INIS)

Ritter, G.A.

1995-01-01

This document summarizes the results of the Phase 1 acceptance test of the 241-SY-101 Flexible Receiver System (FRS). This acceptance test consisted of a pressure-decay/leak test of the containment bag to verify that the seams along the length of the bag had been adequately sealed. The sealing integrity of the FRS must be verified to ensure that the release of waste and aerosols will be minimized during the removal of the test mixer pump from Tank 241-SY-101. The FRS is one of six major components of the Equipment Removal System, which has been designed to retrieve, transport, and store the mixer pump. This acceptance test was performed at Lancs Industries in Kirkland, Washington on January 17, 1995. The bag temperature-compensated pressure loss of 575 Pa was below the acceptance criteria of 625 Pa and the test results were therefore found to be acceptable. The bag manufacturer estimates that 80--90% of the pressure loss is attributed to leakage around the bag inflation valve where the pressure gage was connected. A leak detector was applied over the entire bag during the pre-tests and no leakage was found. Furthermore, the leak rate corresponding to this pressure loss is very small when compared to the acceptable leak rate of the completely assembled FRS. The sealing integrity of the assembled FRS is verified in Phase 3 testing

Effect of graphene oxide on undifferentiated and retinoic acid-differentiated SH-SY5Y cells line

Science.gov (United States)

Lv, Min; Zhang, Yujie; Liang, Le; Wei, Min; Hu, Wenbing; Li, Xiaoming; Huang, Qing

2012-06-01

Graphene oxide (GO), has created an unprecedented opportunity for development and application in biology, due to its abundant functional groups and well water solubility. Recently, the potential toxicity of GO in the environment and in humans has garnered more and more attention. In this paper, we systematically studied the cytotoxicity of GO nanosheets via examining the effect of GO on the morphology, viability and differentiation of a human neuroblastoma SH-SY5Y cell line, which was an ideal model used to study neuronal disease in vitro. The results suggested that GO had no obvious cytotoxicity at low concentration (cells exhibited dose- and time-dependent decreases at high concentration (>=80 μg mL-1). Moreover, GO did not induce apoptosis. Very interestingly, GO significantly enhanced the differentiation of SH-SY5Y induced-retinoic acid (RA) by evaluating neurite length and the expression of neuronal marker MAP2. These data provide a promising application for neurodegenerative diseases.

Protective effect of Pycnogenol in human neuroblastoma SH-SY5Y cells following acrolein-induced cytotoxicity.

Science.gov (United States)

Ansari, Mubeen A; Keller, Jeffrey N; Scheff, Stephen W

2008-12-01

Oxidative stress is one of the hypotheses involved in the etiology of Alzheimer's disease (AD). Considerable attention has been focused on increasing the intracellular glutathione (GSH) levels in many neurodegenerative diseases, including AD. Pycnogenol (PYC) has antioxidant properties and stabilizes intracellular antioxidant defense systems including glutathione levels. The present study investigated the protective effects of PYC on acrolein-induced oxidative cell toxicity in cultured SH-SY5Y neuroblastoma cells. Decreased cell survival in SH-SY5Y cultures treated with acrolein correlated with oxidative stress, increased NADPH oxidase activity, free radical production, protein oxidation/nitration (protein carbonyl, 3-nitrotyrosine), and lipid peroxidation (4-hydroxy-2-nonenal). Pretreatment with PYC significantly attenuated acrolein-induced cytotoxicity, protein damage, lipid peroxidation, and cell death. A dose-response study suggested that PYC showed protective effects against acrolein toxicity by modulating oxidative stress and increasing GSH. These findings provide support that PYC may provide a promising approach for the treatment of oxidative stress-related neurodegenerative diseases such as AD.

Motor skills and school performance in children with daily physical education in school--a 9-year intervention study.

Science.gov (United States)

Ericsson, I; Karlsson, M K

2014-04-01

The aim was to study long-term effects on motor skills and school performance of increased physical education (PE). All pupils born 1990-1992 from one school were included in a longitudinal study over nine years. An intervention group (n = 129) achieved daily PE (5 × 45 min/week) and if needed one extra lesson of adapted motor training. The control group (n = 91) had PE two lessons/week. Motor skills were evaluated by the Motor Skills Development as Ground for Learning observation checklist and school achievements by marks in Swedish, English, Mathematics, and PE and proportion of pupils who qualified for upper secondary school. In school year 9 there were motor skills deficits in 7% of pupils in the intervention group compared to 47% in the control group (P motor skills deficit than among pupils with motor skills deficits (P motor skills training during the compulsory school years is a feasible way to improve not only motor skills but also school performance and the proportion of pupils who qualify for upper secondary school. © 2012 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

A Five-Year School Building and Future Sites Program 1966-1970.

Science.gov (United States)

1965

Five-year school building and site needs and related financial requirements are summarized for Milwaukee's schools. Educational policies concerning the school building program are stated, and consideration is given to factors affecting school board needs such as birth rate, public housing projects, urban renewal, highways, and expressways. School…

System Design Description for the SY-101 Hydrogen Mitigation Test Project Data Acquisition and Control System (DACS-1)

Energy Technology Data Exchange (ETDEWEB)

ERMI, A.M.

2000-01-24

This document describes the hardware and software of the computer subsystems for the Data Acquisition and Control System (DACS) used in mitigation tests conducted on waste tank 241-SY-101 at the Hanford Nuclear Reservation.

Revenues and Expenditures for Public Elementary and Secondary School Districts: School Year 2011-12 (Fiscal Year 2012). First Look. NCES 2014-303

Science.gov (United States)

Cornman, Stephen Q.

2015-01-01

This First Look report presents data on public elementary and secondary education revenues and expenditures at the local education agency (LEA) or school district level for fiscal year (FY) 2012.1. Specifically, this report includes findings from the following types of school finance data: (1) Revenue and expenditure totals by state and the 100…

Revenues and Expenditures for Public Elementary and Secondary School Districts: School Year 2012-13 (Fiscal Year 2013). First Look. NCES 2015-303

Science.gov (United States)

Cornman, Stephen Q.

2016-01-01

This First Look report presents data on public elementary and secondary education revenues and expenditures at the local education agency (LEA) or school district level for fiscal year (FY) 2013. Specifically, this report includes findings from the following types of school finance data: (1) revenue and expenditure totals by state and the 100…

Elementary Technology Demonstration Schools: The Third Year 1992-93. Publication Number 92.31.

Science.gov (United States)

Sabatino, Melissa

The 1992-93 school year was the third year of the Elementary Technology Demonstration Schools program of the Austin (Texas) schools; the project is funded by International Business Machines Corporation (IBM) and Apple Computer Inc. Grants from these corporations were used to equip three elementary schools with IBM equipment and one with Apple…

Lack of Prenylated Proteins, Autophagy Impairment and Apoptosis in SH-SY5Y Neuronal Cell Model of Mevalonate Kinase Deficiency

Directory of Open Access Journals (Sweden)

Paola Maura Tricarico

2017-03-01

Full Text Available Background/Aims: Mevalonate Kinase Deficiency (MKD, is a hereditary disease due to mutations in mevalonate kinase gene (MVK. MKD has heterogeneous clinical phenotypes: the correlation between MVK mutations and MKD clinical phenotype is still to be fully elucidated. Deficiency of prenylated proteins has been hypothesized as possible MKD pathogenic mechanism. Based on this hypothesis and considering that neurologic impairment characterizes Mevalonic Aciduria (MA, the most severe form of MKD, we studied the effects of I268T and N301T MVK mutations on protein prenylation, autophagy and programmed cell death in SH-SY5Y neuroblastoma cell lines. Methods: SH-SY5Y cells were transiently transfected, with the pCMV-6 plasmid containing MVK wild type and the two mutated sequences. Protein prenylation levels were evaluated using GFP-RhoA-F to assess farnesylation, and GFP-RhoA to evaluate geranylgeranylation; autophagy was measured by evaluating LC3 and p62 protein levels, while Annexin V-FITC and Propidium Iodide staining allowed apoptosis detection. Results: MVK mutants’ over-expression causes decreased levels of farnesylation and geranylgeranylation, and also increased LC3 lipidation in SH-SY5Y, with concomitant p62 accumulation. Treatment with bafilomycin A1 (an inhibitor of vacuolar H+-ATPase, a late autophagy inhibitor further increase LC3-II and p62 levels, suggesting that degradation of autophagolysosome could be impaired. SH-SY5Y, with both MVK mutants, showed apoptosis increase; the presence of N301T associated with augmented cell death. Conclusions: We hypothesize that mevalonate pathway impairment causes alteration of farnesylation and geranylgeranylation proteins and alteration of the autophagic flux; these changes can induce apoptosis, possibly more relevant in the presence of N301T mutation.

Lack of Prenylated Proteins, Autophagy Impairment and Apoptosis in SH-SY5Y Neuronal Cell Model of Mevalonate Kinase Deficiency.

Science.gov (United States)

Tricarico, Paola Maura; Romeo, Alessandra; Gratton, Rossella; Crovella, Sergio; Celsi, Fulvio

2017-01-01

Mevalonate Kinase Deficiency (MKD), is a hereditary disease due to mutations in mevalonate kinase gene (MVK). MKD has heterogeneous clinical phenotypes: the correlation between MVK mutations and MKD clinical phenotype is still to be fully elucidated. Deficiency of prenylated proteins has been hypothesized as possible MKD pathogenic mechanism. Based on this hypothesis and considering that neurologic impairment characterizes Mevalonic Aciduria (MA), the most severe form of MKD, we studied the effects of I268T and N301T MVK mutations on protein prenylation, autophagy and programmed cell death in SH-SY5Y neuroblastoma cell lines. SH-SY5Y cells were transiently transfected, with the pCMV-6 plasmid containing MVK wild type and the two mutated sequences. Protein prenylation levels were evaluated using GFP-RhoA-F to assess farnesylation, and GFP-RhoA to evaluate geranylgeranylation; autophagy was measured by evaluating LC3 and p62 protein levels, while Annexin V-FITC and Propidium Iodide staining allowed apoptosis detection. MVK mutants' over-expression causes decreased levels of farnesylation and geranylgeranylation, and also increased LC3 lipidation in SH-SY5Y, with concomitant p62 accumulation. Treatment with bafilomycin A1 (an inhibitor of vacuolar H+-ATPase, a late autophagy inhibitor) further increase LC3-II and p62 levels, suggesting that degradation of autophagolysosome could be impaired. SH-SY5Y, with both MVK mutants, showed apoptosis increase; the presence of N301T associated with augmented cell death. We hypothesize that mevalonate pathway impairment causes alteration of farnesylation and geranylgeranylation proteins and alteration of the autophagic flux; these changes can induce apoptosis, possibly more relevant in the presence of N301T mutation. © 2017 The Author(s)Published by S. Karger AG, Basel.

Neuroprotective effects of Salvia aristata Aucher ex Benth. on hydrogen peroxide induced apoptosis in SH-SY5Y neuroblastoma cells

Directory of Open Access Journals (Sweden)

M. A. Esmaeili

2015-08-01

Full Text Available Background and objectives: Oxidative stress is implicated in the neuronal damage associated with Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, amyotropic lateral sclerosis and cerebral ischemic stroke. The present work was designed to establish the neuroprotective effects of Salvia aristata extract on H2O2-induced apoptosis in human dopaminergic SH-SY5Y cells. Methods: The total phenol and flavonoids contents of the plant extracts were quantified by colorimetric methods. The antioxidant activity was assessed using DPPH free radicals scavenging activity assay, and the neuroprotective effect on H2O2-induced oxidative stress was also investigated using human dopaminergic SH-SY5Y cells by MTT assay and western blotting techniques. Results: The highest scavenging activity was found for methanol extract of S. aristata roots (85.28 ± 2.61 μg/mL, with the highest total phenolic and flavonoids content (90.28 mg total phenols as gallic acid and 250.12 mg total flavonoids as rutin, respectively. Our results also, showed that H2O2-induced cytotoxicity in SH-SY5Y cells was suppressed by treatment with S. aristata. Moreover, S. aristata root extract was effective in attenuating the disruption of mitochondrial membrane potential and apoptotic cell death has induced by H2O2.  S. aristata suppressed the down-regulation of Bcl-2, upregulation of Bax, and the release of mitochondrial cytochrome c to cytosol. In addition, S. aristata attenuated caspase-3, and -9 activation, and eventually protected the cells against H2O2-induced apoptosis. Conclusion: Theresults of the present study suggest that treatment of SH-SY5Y cells with S. aristata could block H2O2-induced apoptosis by regulating Bcl-2 family members and by suppressing caspase cascade activation.

Waldorf Schools: Seventy-Six Years of Early Language Learning.

Science.gov (United States)

Navascues, Michael

1997-01-01

Describes the history, curriculum, and methodology of elementary school foreign-language (FL) learning within Waldorf schools, using information from Waldorf FL teachers, class observations, and research readings. Waldorf students study two FLs. An oral/choral method is used in the early years. Reading, writing, and formal grammar are introduced…

Providing Retraining and Advancement Training for Primary/Elementary School Teachers at the State Level in Great Britain and the USA

OpenAIRE

Chychuk Antonina

2017-01-01

In Great Britain and the USA the normative basis of primary/elementary school teachers’ qualification advancement is being actively developed, i. e. this issue is considered at the state level. For a long time the development of retraining and advancement training system for primary/elementary school teachers has been grounded on conceptual foundations of pedagogy that ensures the functionality of the mentioned system. The research results on conceptual foundations for forming an education sy...

The Neuroprotective Effects of Brazilian Green Propolis on Neurodegenerative Damage in Human Neuronal SH-SY5Y Cells

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Junjun Ni

2017-01-01

Full Text Available Oxidative stress and synapse dysfunction are the major neurodegenerative damage correlated to cognitive impairment in Alzheimer’s disease (AD. We have found that Brazilian green propolis (propolis improves the cognitive functions of mild cognitive impairment patients living at high altitude; however, mechanism underlying the effects of propolis is unknown. In the present study, we investigated the effects of propolis on oxidative stress, expression of brain-derived neurotrophic factor (BDNF, and activity-regulated cytoskeleton-associated protein (Arc, the critical factors of synapse efficacy, using human neuroblastoma SH-SY5Y cells. Pretreatment with propolis significantly ameliorated the hydrogen peroxide- (H2O2- induced cytotoxicity in SH-SY5Y cells. Furthermore, propolis significantly reduced the H2O2-generated reactive oxygen species (ROS derived from mitochondria and 8-oxo-2′-deoxyguanosine (8-oxo-dG, the DNA oxidative damage marker but significantly reversed the fibrillar β-amyloid and IL-1β-impaired BDNF-induced Arc expression in SH-SY5Y cells. Furthermore, propolis significantly upregulated BDNF mRNA expression in time- and dose-dependent manners. In addition, propolis induced Arc mRNA and protein expression via phosphoinositide-3 kinase (PI3K. These observations strongly suggest that propolis protects from the neurodegenerative damage in neurons through the properties of various antioxidants. The present study provides a potential molecular mechanism of Brazilian green propolis in prevention of cognitive impairment in AD as well as aging.

Effects of a 2-year school-based daily physical activity intervention on cardiorespiratory fitness: the Sogndal school-intervention study

DEFF Research Database (Denmark)

Resaland, G K; Andersen, Lars Bo; Mamen, A

2011-01-01

The aim of this study was to describe changes in children's cardiorespiratory fitness (CRF) following a school-based physical activity (PA) intervention. In total, 259 children (age 9.3+/-0.3 years) were invited to participate, of whom 256 participated. The children from the intervention school (63...... boys, 62 girls) carried out 60-min PA over 2 school years. The children from the control school (62 boys, 69 girls) had the regular curriculum-defined amount of physical education in school, i.e. 45 min twice weekly. One hundred and eighty-eight children (73.4%) successfully completed both the baseline...

Presenilin-1 mutations alter K+ currents in the human neuroblastoma cell line, SH-SY5Y

DEFF Research Database (Denmark)

Plant, Leigh D; Boyle, John P; Thomas, Natasha M

2002-01-01

Mutations in presenilin 1 (PS1) are the major cause of autosomal dominant Alzheimer's disease. We have measured the voltage-gated K+ current in the human neuroblastoma cell line SH-SY5Y using whole-cell patch-clamp. When cells were stably transfected to over-express PS1, no change in K+ current...

Thermocouple module halt failure acceptance test procedure for Tank 241-SY-101 DACS-1

International Nuclear Information System (INIS)

Ermi, A.M.

1997-01-01

The readiness of the Tank 241-SY-101 Data Acquisition and Control System (DACS-1) to provide monitoring and alarms for a halt failure of any thermocouple module will be tested during the performance of this procedure. Updated DACS-1 ''1/0 MODULE HEALTH STATUS'', ''MININ1'', and ''MININ2'' screens, which now provide indication of thermocouple module failure, will also be tested as part of this procedure

A comparison of genotyping-by-sequencing analysis methods on low-coverage crop datasets shows advantages of a new workflow, GB-eaSy.

Science.gov (United States)

Wickland, Daniel P; Battu, Gopal; Hudson, Karen A; Diers, Brian W; Hudson, Matthew E

2017-12-28

Genotyping-by-sequencing (GBS), a method to identify genetic variants and quickly genotype samples, reduces genome complexity by using restriction enzymes to divide the genome into fragments whose ends are sequenced on short-read sequencing platforms. While cost-effective, this method produces extensive missing data and requires complex bioinformatics analysis. GBS is most commonly used on crop plant genomes, and because crop plants have highly variable ploidy and repeat content, the performance of GBS analysis software can vary by target organism. Here we focus our analysis on soybean, a polyploid crop with a highly duplicated genome, relatively little public GBS data and few dedicated tools. We compared the performance of five GBS pipelines using low-coverage Illumina sequence data from three soybean populations. To address issues identified with existing methods, we developed GB-eaSy, a GBS bioinformatics workflow that incorporates widely used genomics tools, parallelization and automation to increase the accuracy and accessibility of GBS data analysis. Compared to other GBS pipelines, GB-eaSy rapidly and accurately identified the greatest number of SNPs, with SNP calls closely concordant with whole-genome sequencing of selected lines. Across all five GBS analysis platforms, SNP calls showed unexpectedly low convergence but generally high accuracy, indicating that the workflows arrived at largely complementary sets of valid SNP calls on the low-coverage data analyzed. We show that GB-eaSy is approximately as good as, or better than, other leading software solutions in the accuracy, yield and missing data fraction of variant calling, as tested on low-coverage genomic data from soybean. It also performs well relative to other solutions in terms of the run time and disk space required. In addition, GB-eaSy is built from existing open-source, modular software packages that are regularly updated and commonly used, making it straightforward to install and maintain

Parent involvement in beginning primary school: Correlates and changes in involvement across the first two years of school in a New Zealand sample.

Science.gov (United States)

McDowall, Philippa S; Taumoepeau, Mele; Schaughency, Elizabeth

2017-06-01

This study described the relations of parents' and teachers' beliefs and attitudes to forms of parents' involvement in children's first two years of primary school. Parents of children in their first year of primary school (age 5) were recruited from 12 classrooms within four schools in New Zealand; 196 families participated in their child's first year, and 124 families continued to participate in their child's second school year. Parents completed the Family-Involvement Questionnaire, New Zealand, and we archivally collected parent-documented children's oral reading homework. Teachers' rated helpfulness of parents' involvement at school (level 2) and parents' rated teacher invitations to be involved and their perceived time and energy (level 1) contributed to school-based involvement in Year 1 in multilevel models, with parents' rated teacher invitations for involvement also found to predict Year 1 home-school communication in regression analyses. Contributors to Year 1 child-parent reading in multilevel models included level 1 predictors of two or more adults in the home and parents' perceived time and energy. Longitudinal analyses suggested both consistency and change in each form of involvement from Year 1 to Year 2, with increases in each form of involvement found to be associated with increases in parents' and/or teachers' views about involvement in Year 2 in cross-sectional time-series analyses. Implications for schools wanting to engage families are that parents' involvement in children's schooling may be influenced by parents' perceptions of their capacity, teachers' engagement efforts, and the school's climate for involvement. This is a special issue paper "Family Engagement in Education and Intervention". Copyright © 2017 Society for the Study of School Psychology. Published by Elsevier Ltd. All rights reserved.

Effects of Year-Round Education on Texas Middle School Student Performance

Science.gov (United States)

Coopersmith, Michael

2011-01-01

This study was designed to investigate the effects of the year-round calendar on student performance in Texas middle schools as measured by achievement on the Texas Assessment of Knowledge and Skills (TAKS) test. In the State of Texas, 15 schools served students in grades six through eight using the year-round calendar in 2009-2010. The 15…

The Readiness of High School Students to Pursue First Year Physics

Science.gov (United States)

Ramnarain, U.; Molefe, P.

2012-01-01

A high failure rate at first year physics is often attributed to the lack of readiness of high school students to pursue such studies. This research explores this issue and reports on the perceptions of five physics lecturers at a South African university on the preparedness of high school students for first year physics. Qualitative data was…

Physical activity level of school children of age 10-13 years

OpenAIRE

Ronghe, Dr. Rashmi N; Gotmare, Dr. Neha A; Kawishwar, Dr. Shraddha

2016-01-01

Aim: To assess physical activity level of school children of age 10-13 years.Objectives: To assess and grade physical activity level in children of age 10-13 years using Physical Activity Questionnaire for Children (PAQ-C) classified into: Light Physical activity; Moderate Physical activity; Moderate to vigorous Physical activity and High Physical activity.Methodology: This is Questionnaire based survey study which was conducted on 100 school going children of 10-13 years who were present on ...

Effect of ellagic acid on proliferation, cell adhesion and apoptosis in SH-SY5Y human neuroblastoma cells.

Science.gov (United States)

Fjaeraa, Christina; Nånberg, Eewa

2009-05-01

Ellagic acid, a polyphenolic compound found in berries, fruits and nuts, has been shown to possess growth-inhibiting and apoptosis promoting activities in cancer cell lines in vitro. The objective of this study was to investigate the effect of ellagic acid in human neuroblastoma SH-SY5Y cells. In cultures of SH-SY5Y cells incubated with ellagic acid, time- and concentration-dependent inhibitory effects on cell number were demonstrated. Ellagic acid induced cell detachment, decreased cell viability and induced apoptosis as measured by DNA strand breaks. Ellagic acid-induced alterations in cell cycle were also observed. Simultaneous treatment with all-trans retinoic acid did not rescue the cells from ellagic acid effects. Furthermore, the results suggested that pre-treatment with all-trans retinoic acid to induce differentiation and cell cycle arrest did not rescue the cells from ellagic acid-induced cell death.

An evaluation of vocational high schools in Indonesia: A comparison between four-year and three-year programs

Directory of Open Access Journals (Sweden)

Soenarto Soenarto

2017-12-01

Full Text Available The research aimed to gain insights into the quality of four-year program vocational high school (VHS in Indonesia compared to four-year program VHS. This research was conducted based on the school graduate standard, business sector and industrial sector (or Dunia Usaha dan Dunia Industri (DUDI – or the performance of the graduates and alumni (the graduates’ satisfaction. The research was conducted using Discrepancy Evaluation Model using 16 VHSs (eight four-year program VHSs and eight three-year program VHSs. The result shows that from the standpoint of the school, the graduates of the four-year program VHS are higher in quality than those of the three-year program VHS. The four-year program VHS graduates are more qualified in seven aspects: teamwork, discipline, tenacity, theoretical knowledge, confidence, creativity, and leadership. Meanwhile, using DUDI standpoint, the four-year program VHS graduates are also higher in quality than the three-year program VHS graduates. In addition, the four-year program VHS graduates are better in the quality of their discipline, tenacity, theoretical knowledge, practical skills, confidence, carefulness, creativity, and leadership. The four-year program VHS graduates have a higher level of satisfaction in terms of income than the three-year program VHS graduates. The higher quality of the four-year program VHS graduates has resulted from longer duration of the internship program (PKL that provides them with reliable experience and skills concerning work-related problem-solving activities.

JNK signaling pathway regulates sorbitol-induced Tau proteolysis and apoptosis in SH-SY5Y cells by targeting caspase-3.

Science.gov (United States)

Olivera Santa-Catalina, Marta; Caballero Bermejo, Montaña; Argent, Ricardo; Alonso, Juan C; Centeno, Francisco; Lorenzo, María J

2017-12-15

Growing evidence suggests that Diabetes Mellitus increases the risk of developing Alzheimer's disease. It is well known that hyperglycemia, a key feature of Diabetes Mellitus, may induce plasma osmolarity disturbances. Both hyperglycemia and hyperosmolarity promote the altered post-translational regulation of microtubule-associated protein Tau. Interestingly, abnormal hyperphosphorylation and cleavage of Tau have been proven to lead to the genesis of filamentous structures referred to as neurofibrillary tangles, the main pathological hallmark of Alzheimer's disease. We have previously described that hyperosmotic stress induced by sorbitol promotes Tau proteolysis and apoptosis in SH-SY5Y cells via caspase-3 activation. In order to gain insights into the regulatory mechanisms of such processes, in this work we explored the intracellular signaling pathways that regulate these events. We found that sorbitol treatment significantly enhanced the activation of conventional families of MAPK in SH-SY5Y cells. Tau proteolysis was completely prevented by JNK inhibition but not affected by either ERK1/2 or p38 MAPK blockade. Moreover, inhibition of JNK, but not ERK1/2 or p38 MAPK, efficiently prevented sorbitol-induced apoptosis and caspase-3 activation. In summary, we provide evidence that JNK signaling pathway is an upstream regulator of hyperosmotic stress-induced Tau cleavage and apoptosis in SH-SY5Y through the control of caspase-3 activation. Copyright © 2017 Elsevier Inc. All rights reserved.

Preventing and Responding to Bullying: An Elementary School's 4-Year Journey

Science.gov (United States)

McCormac, Mary E.

2015-01-01

Bullying continues to be a pervasive problem in schools and requires a schoolwide approach. This article describes the action research process used to examine the impact of a 4-year, K-5 school bullying prevention and intervention. The school counselors collaborated with students, staff, and parents to implement the program, and collected and…

Problems catching up – age disadvantage in letter recognition, phoneme awareness and speed naming in Danish five-year-olds at school entry does not disappear after the first school year

DEFF Research Database (Denmark)

Wilms, Inge Linda

2017-01-01

(a) Research findings: Purpose: The purpose of the study was to investigate the development in mastery of preparatory reading skills at the first school year. Method: 96 children from a high income, high resource area of Denmark were tested on alphabet knowledge, phonemic awareness, word mobiliza......(a) Research findings: Purpose: The purpose of the study was to investigate the development in mastery of preparatory reading skills at the first school year. Method: 96 children from a high income, high resource area of Denmark were tested on alphabet knowledge, phonemic awareness, word...... mobilization and word reading at the entry and exit to school (0-grade). Results: There are significant differences in preparatory reading skills in five and six year old children. The differences in performance scores are not evened out during the first school year but remain the same. Conclusion: Even...... with intensive day care and similar demographical and socio-economical factors, children will start school with different levels of preparatory reading skills depending on entry to school age. The Danish curriculum which favors standardized reading practice will not be sufficient to even out skills differences...

A Time Profile of Mathematics in a "Gap Year" in Irish Secondary Schools

Science.gov (United States)

Prendergast, Mark; O'Meara, Niamh

2016-01-01

The Irish education system is unique in an international context as it sets aside a full school year for a transition and youth development programme in the middle of secondary education. The Transition Year (TY) programme is an optional, full time programme offered in the majority of secondary schools. Each school designs its own programme,…

Effects of a 2-year school-based daily physical activity intervention on cardiovascular disease risk factors: the Sogndal school-intervention study

DEFF Research Database (Denmark)

Resaland, G K; Anderssen, S A; Holme, I M

2011-01-01

at the I-school carried out 60 min of PA daily. The PA lessons were planned, organized and led by expert physical education (PE) teachers. In the C-school, children were offered the normal 45 min of PE twice weekly. The intervention resulted in a greater beneficial development in systolic (P=0......The aim of this study was to investigate the effect of a 2-year school-based physical activity (PA) intervention in 9-year-old children on cardiovascular disease (CVD) risk factors. One intervention school (I-school) (n=125) and one control school (C-school) (n=131) were included. The children...

Health education in primary school textbooks in iran in school year 2010-2011.

Directory of Open Access Journals (Sweden)

Reza Kazemian

2014-10-01

Full Text Available Health education in schools is one of the most effective ways of promoting health in a society. Studies have shown the effectiveness of health interventions aimed at improving students' knowledge, attitude, and behaviors about health issues. The aim of this study was to evaluate health issues in primary school textbooks in Iran.In school year 2010-2011, the contents of all primary school textbooks in Iran were assessed in accordance to their health-related teachings. Health lessons of these 27 textbooks in the form of picture and text were retrieved and analyzed using content analysis method.In total, 502 health-related lessons were found. The textbooks of the third grade contained the highest (144 and those of the fourth grade had the lowest (26 number of health lessons. Among health-related issues, the largest number (87 of lessons were about personal hygiene, while prevention of high risk behaviors comprised the least number (8. Some important health issues such as nutrition, oral health, and prevention of high-risk behaviors were not adequately discussed in the textbooks.The potential of primary school textbooks in delivering health messages has been neglected in Iran. Taking the critical importance of school ages into account, incorporating health issues in textbooks should be more strongly emphasized.

Health education in primary school textbooks in iran in school year 2010-2011.

Science.gov (United States)

Kazemian, Reza; Ghasemi, Hadi; Movahhed, Taraneh; Kazemian, Ali

2014-09-01

Health education in schools is one of the most effective ways of promoting health in a society. Studies have shown the effectiveness of health interventions aimed at improving students' knowledge, attitude, and behaviors about health issues. The aim of this study was to evaluate health issues in primary school textbooks in Iran. In school year 2010-2011, the contents of all primary school textbooks in Iran were assessed in accordance to their health-related teachings. Health lessons of these 27 textbooks in the form of picture and text were retrieved and analyzed using content analysis method. In total, 502 health-related lessons were found. The textbooks of the third grade contained the highest (144) and those of the fourth grade had the lowest (26) number of health lessons. Among health-related issues, the largest number (87) of lessons were about personal hygiene, while prevention of high risk behaviors comprised the least number (8). Some important health issues such as nutrition, oral health, and prevention of high-risk behaviors were not adequately discussed in the textbooks. The potential of primary school textbooks in delivering health messages has been neglected in Iran. Taking the critical importance of school ages into account, incorporating health issues in textbooks should be more strongly emphasized.

Acceptance test report, 241-SY-101 Flexible Receiver System, Phase 2 testing

International Nuclear Information System (INIS)

Ritter, G.A.

1995-01-01

This document summarizes the results of the Phase 2 acceptance test of the 241-SY-101 Flexible Receiver System (FRS). The FRS is one of six major components of the Equipment Removal System, which has been designed to retrieve, transport, and store the test mixer pump currently installed in Tank 241-SY-101. The purpose of this acceptance test is to verify the strength of the containment bag and bag bottom cinching mechanism. It is postulated that 68 gallons of waste could be trapped inside the pump internals. The bag must be capable of supporting this waste if it shakes loose and drains to the bottom of the bag after the bag bottom has been cinched closed. This acceptance test was performed at the Maintenance and Storage Facility (MASF) Facility in the 400 area on January 23, 1995. The bag assembly supported the weight of 920 kg (2,020 lbs) of water with no leakage or damage to the bag. This value meets the acceptance criteria of 910 kg of water and therefore the results were found to be acceptable. The maximum volume of liquid expected to be held up in the pump internals is 258 L (68 gallons), which corresponds to 410 kg. This test weight gives just over a safety factor of 2. The bag also supported a small shock load while it was filled with water when the crane hoisted the bag assembly up and down. Based on the strength rating of the bag components, the bag assembly should support 2--3 times the test weight of 910 kg

Activated cathepsin L is associated with the switch from autophagy to apoptotic death of SH-SY5Y cells exposed to 6-hydroxydopamine

Energy Technology Data Exchange (ETDEWEB)

Li, Lingyun, E-mail: lingyunlee@126.com [Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123 (China); Experimental Center, The Second Affiliated Hospital of Soochow University, Suzhou 215004 (China); Gao, Luyan [Experimental Center, The Second Affiliated Hospital of Soochow University, Suzhou 215004 (China); Song, Yunzhen; Qin, Zheng-Hong [Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123 (China); Liang, Zhongqin, E-mail: liangzhongqin@suda.edu.cn [Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123 (China)

2016-02-12

Autophagy and apoptosis are common responses to pathological damage in the process of Parkinson's disease (PD), and lysosome dysfunction may contribute to the etiology of PD's neurodegenerative process. In this study, we demonstrated that the neurotoxin 6-hydroxydopamine (6-OHDA) increased autophagy in SH-SY5Y cells, as determined by detection of the lysosome marker lysosomal-associated membrane protein1, the autophagy protein light chain 3 (LC3)-II and the autophagy substrate P62 protein. Meanwhile, autophagy repression with 3-methyladenine accelerated the activation of caspase-3 and PARP and aggravated the cell apoptotic death induced by 6-OHDA. Furthermore, we found that 6-OHDA treatment resulted in a transient increase in the intracellular and nuclear expression of cathepsin L (CTSL). The CTSL inhibitor, Z-FY-CHO, could promote autophagy, decrease accumulation of P62, and block activation of caspase-3 and PARP. Taken together, these results suggest that activation of autophagy may primarily be a protective process in SH-SY5Y cell death induced by 6-OHDA, and the nuclear translocation of CTSL could enhance the cell apoptotic cascade via disturbing autophagy-apoptotic systems in SH-SY5Y cells. Our findings highlight the potential role of CTSL in the cross talk between autophagy and apoptosis, which might be considered a therapeutic strategy for treatment of pathologic conditions associated with neurodegeneration. - Highlights: • Inhibition of autophagy aggravated the cell apoptotic death in SH-SY5Y cells. • Activation of cathepsin L impaired the autophagy pathway. • Activation of cathepsin L enhanced the cell apoptotic cascade. • Cathepsin L involves in the cross talk between autophagy and apoptosis.

Impact of School Desegregation in Milwaukee Public Schools on Quality Education for Minorities... 15 Years Later.

Science.gov (United States)

Wisconsin Advisory Committee to the United States Commission on Civil Rights.

This publication reports on the effects of school desegregation in Milwaukee (Wisconsin) Public Schools 15 years after desegregation was introduced and focuses on the quality of education available for minorities. In particular, the report looks at desegregation and educational outcomes, interracial and human relations, the effect of housing…

WHO European Childhood Obesity Surveillance Initiative: body mass index and level of overweight among 6-9-year-old children from school year 2007/2008 to school year 2009/2010.

LENUS (Irish Health Repository)

Wijnhoven, Trudy Ma

2014-01-01

The World Health Organization (WHO) Regional Office for Europe has established the Childhood Obesity Surveillance Initiative (COSI) to monitor changes in overweight in primary-school children. The aims of this paper are to present the anthropometric results of COSI Round 2 (2009\\/2010) and to explore changes in body mass index (BMI) and overweight among children within and across nine countries from school years 2007\\/2008 to 2009\\/2010.

Interleukin-18 alters protein expressions of neurodegenerative diseases-linked proteins in human SH-SY5Y neuron-like cells

Directory of Open Access Journals (Sweden)

Elina M Sutinen

2014-08-01

Full Text Available Chronic inflammation and oxidative stress (OS are present in Alzheimer´s disease (AD brains in addition to neuronal loss, Amyloid-β (Aβ plaques and hyperphosphorylated tau-protein neurofibrillary tangles. Previously we showed that levels of the pro-inflammatory cytokine, interleukin-18 (IL-18, are elevated in post-mortem AD brains. IL-18 can modulate the tau kinases, Cdk5 and GSK3β, as well as Aβ-production. IL-18 levels are also increased in AD risk diseases, including type-2 diabetes and obesity. Here, we explored other IL-18 regulated proteins in neuron-like SH-SY5Y cells. Differentiated SH-SY5Y cells, incubated with IL-18 for 24, 48 or 72h, were analyzed by two-dimensional gel electrophoresis (2D-DIGE. Specific altered protein spots were chosen and identified with mass spectrometry and verified by western immunoblotting. IL-18 had time-dependent effects on the SH-SY5Y proteome, modulating numerous protein levels/modifications. We concentrated on those related to OS (DDAH2, peroxiredoxins 2, 3 and 6, DJ-1, BLVRA, Aβ-degradation (MMP14, TIMP2, Aβ-aggregation (Septin-2 and modifications of axon growth and guidance associated, collapsing response mediator protein 2 (CRMP2. IL-18 significantly increased antioxidative enzymes, indicative of OS, and altered levels of glycolytic α- and γ-enolase and multifunctional 14-3-3γ and -ε, commonly affected in neurodegenerative diseases. MMP14, TIMP2, α-enolase and 14-3-3ε, indirectly involved in Aβ metabolism, as well as Septin-2 showed changes that increase Aβ levels. Increased 14-3-3γ may contribute to GSK3β driven tau hyperphosphorylation and CRMP2 Thr514 and Ser522 phosphorylation with the Thr555-site, a target for Rho kinase, showing time-dependent changes. IL-18 also increased caspase-1 levels and vacuolization of the cells. Although our SH-SY5Y cells were not aged, as neurons in AD, our work suggests that heightened or prolonged IL-18 levels can drive protein changes of known

Rethinking Dental School Admission Criteria: Correlation Between Pre-Admission Variables and First-Year Performance for Six Classes at One Dental School.

Science.gov (United States)

Rowland, Kevin C; Rieken, Susan

2018-04-01

Admissions committees in dental schools are charged with the responsibility of selecting candidates who will succeed in school and become successful members of the profession. Identifying students who will have academic difficulty is challenging. The aim of this study was to determine the predictive value of pre-admission variables for the first-year performance of six classes at one U.S. dental school. The authors hypothesized that the variables undergraduate grade point average (GPA), undergraduate science GPA (biology, chemistry, and physics), and Dental Admission Test (DAT) scores would predict the level of performance achieved in the first year of dental school, measured by year-end GPA. Data were collected in 2015 from school records for all 297 students in the six cohorts who completed the first year (Classes of 2007 through 2013). In the results, statistically significant correlations existed between all pre-admission variables and first-year GPA, but the associations were only weak to moderate. Lower performing students at the end of the first year (lowest 10% of GPA) had, on average, lower pre-admission variables than the other students, but the differences were small (≤10.8% in all categories). When all the pre-admission variables were considered together in a multiple regression analysis, a significant association was found between pre-admission variables and first-year GPA, but the association was weak (adjusted R 2 =0.238). This weak association suggests that these students' first-year dental school GPAs were mostly determined by factors other than the pre-admission variables studied and has resulted in the school's placing greater emphasis on other factors for admission decisions.

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line.

Science.gov (United States)

Shipley, Mackenzie M; Mangold, Colleen A; Szpara, Moriah L

2016-02-17

Having appropriate in vivo and in vitro systems that provide translational models for human disease is an integral aspect of research in neurobiology and the neurosciences. Traditional in vitro experimental models used in neurobiology include primary neuronal cultures from rats and mice, neuroblastoma cell lines including rat B35 and mouse Neuro-2A cells, rat PC12 cells, and short-term slice cultures. While many researchers rely on these models, they lack a human component and observed experimental effects could be exclusive to the respective species and may not occur identically in humans. Additionally, although these cells are neurons, they may have unstable karyotypes, making their use problematic for studies of gene expression and reproducible studies of cell signaling. It is therefore important to develop more consistent models of human neurological disease. The following procedure describes an easy-to-follow, reproducible method to obtain homogenous and viable human neuronal cultures, by differentiating the chromosomally stable human neuroblastoma cell line, SH-SY5Y. This method integrates several previously described methods(1-4) and is based on sequential removal of serum from media. The timeline includes gradual serum-starvation, with introduction of extracellular matrix proteins and neurotrophic factors. This allows neurons to differentiate, while epithelial cells are selected against, resulting in a homogeneous neuronal culture. Representative results demonstrate the successful differentiation of SH-SY5Y neuroblastoma cells from an initial epithelial-like cell phenotype into a more expansive and branched neuronal phenotype. This protocol offers a reliable way to generate homogeneous populations of neuronal cultures that can be used for subsequent biochemical and molecular analyses, which provides researchers with a more accurate translational model of human infection and disease.

Insulin-like Growth Factor-I Mediates Neuroprotection in Proteasome Inhibition-Induced Cytotoxicity in SH-SY5Y Cells

Science.gov (United States)

Cheng, Benxu; Maffi, Shivani Kaushal; Martinez, Alex Anthony; Acosta, Yolanda P Villarreal; Morales, Liza D; Roberts, James L

2011-01-01

The proteasome is an enzyme complex responsible for targeted intracellular proteolysis. Alterations in proteasome-mediated protein clearance have been implicated in the pathogenesis of aging, Alzheimer's disease (AD) and Parkinson's disease (PD). In such diseases, proteasome inhibition may contribute to formation of abnormal protein aggregates, which in turn activate intracellular unfolded protein responses that cause oxidative stress and apoptosis. In this study, we investigated the protective effect of Insulin-like Growth Factor-I (IGF-1) for neural SH-SY5Y cells treated with the proteasomal inhibitor, Epoxomicin, In SH-SY5Y cells, Epoxomicin treatment results in accumulation of intracellular ubiquitinated proteins and cytochrome c release from damaged mitochondria, leading to cell death, in Epoxomicin time- and dose-dependent manner. In cells treated with small amounts of IGF-1, the same dosages of Epoxomicin reduced both mitochondrial damage (cytochrome c release) and reduced caspase-3 activation and PARP cleavage, both of which are markers of apoptosis. Notably, however, IGF-1-treated SH-SY5Y cells still contained ubiquitinated protein aggregates. This result indicates that IGF-1 blocks the downstream apoptotic consequences of Epoxomicin treatment leading to decreased proteasome function. Clues as to the mechanism for this protective effect come from (a) increased AKT phosphorylation observed in IGF-1-protected cells, vs. cells exposed to Epoxomicin without IGF-1, and (b) reduction of IGF-1 protection by pretreatment of the cells with LY294002 (an inhibitor of PI3-kinase). Together these findings suggest that activation of PI3/AKT pathways by IGF-1 is involved in IGF-1 neuroprotection against apoptosis following proteasome inhibition. PMID:21545837

An Evaluation of the Model School Division (MSD) Preschool Program for the School Year 1973-74.

Science.gov (United States)

District of Columbia Public Schools, Washington, DC. Dept. of Research and Evaluation.

This study was designed to assess the extent to which children served by the Model Schools Division Preschool Program developed socially, intellectually, physically and emotionally during the 1973-74 school year. This evaluation was also designed to measure the appropriateness of the learning environment and the amount of services provided by the…

BDNF and the maturation of posttranscriptional regulatory networks in human SH-SY5Y neuroblast differentiation

Directory of Open Access Journals (Sweden)

Belinda J Goldie

2014-10-01

Full Text Available The SH-SY5Y culture system is a convenient neuronal model with the potential to elaborate human/primate-specific transcription networks and pathways related to human cognitive disorders. While this system allows for the exploration of specialised features in the human genome, there is still significant debate about how this model should be implemented, and its appropriateness for answering complex functional questions related to human neural architecture. In view of these questions we sought to characterise the posttranscriptional regulatory structure of the two-stage ATRA differentiation, BDNF maturation protocol proposed by Encinas and colleagues (2010 using integrative whole-genome gene and microRNA (miRNA expression analysis. We report that ATRA-BDNF induced significant increases in expression of key synaptic genes, brain-specific miRNA and miRNA biogenesis machinery, and in AChE activity, compared with ATRA alone. Functional annotation clustering associated BDNF more significantly with neuronal terms, and with synaptic terms not found in ATRA-only clusters. While our results support use of SH-SY5Y as a neuronal model, we advocate considered selection of the differentiation agent/s relative to the system being modelled.

BDNF and the maturation of posttranscriptional regulatory networks in human SH-SY5Y neuroblast differentiation.

Science.gov (United States)

Goldie, Belinda J; Barnett, Michelle M; Cairns, Murray J

2014-01-01

The SH-SY5Y culture system is a convenient neuronal model with the potential to elaborate human/primate-specific transcription networks and pathways related to human cognitive disorders. While this system allows for the exploration of specialized features in the human genome, there is still significant debate about how this model should be implemented, and its appropriateness for answering complex functional questions related to human neural architecture. In view of these questions we sought to characterize the posttranscriptional regulatory structure of the two-stage ATRA differentiation, BDNF maturation protocol proposed by Encinas et al. (2000) using integrative whole-genome gene and microRNA (miRNA) expression analysis. We report that ATRA-BDNF induced significant increases in expression of key synaptic genes, brain-specific miRNA and miRNA biogenesis machinery, and in AChE activity, compared with ATRA alone. Functional annotation clustering associated BDNF more significantly with neuronal terms, and with synaptic terms not found in ATRA-only clusters. While our results support use of SH-SY5Y as a neuronal model, we advocate considered selection of the differentiation agent/s relative to the system being modeled.

Analysis of the Impacts of City Year's Whole School Whole Child Model on Partner Schools' Performance

Science.gov (United States)

Meredith, Julie; Anderson, Leslie M.

2015-01-01

City Year is a learning organization committed to the rigorous evaluation of its "Whole School Whole Child" model, which trains and deploys teams of AmeriCorps members to low-performing, urban schools to empower more students to reach their full potential. A third-party study by Policy Studies Associates (PSA) examined the impact of…

Hanford Site Tank 241-SY-101, damaged equipment removal

International Nuclear Information System (INIS)

Titzler, P.A.; Legare, D.E.; Barrus, H.G.

1993-11-01

Hanford Site Tank 241-SY-101 has a history of generating hydrogen-nitrous oxide gases. The gases are generated and trapped in the non-convective waste layer near the bottom of the 23-m- (75-ft-) diameter underground tank. Approximately every three months the pressure in the tank is relieved as the trapped gases are released through or around the surface crust into the tank dome. This process moves large amounts of liquid waste and crust material around in the tank. The moving waste displaced air lances and thermocouple assemblies (2-in. schedule-40 pipe) installed in four tank risers and permanently bent them to a maximum angle of 40 degrees. The bends were so severe that assemblies could not be removed from the tank using the originally designed hardware. Just after the tank releases the trapped gas, a 20-to-30-day work ''window'' opens

FGF1 protects neuroblastoma SH-SY5Y cells from p53-dependent apoptosis through an intracrine pathway regulated by FGF1 phosphorylation

Science.gov (United States)

Pirou, Caroline; Montazer-Torbati, Fatemeh; Jah, Nadège; Delmas, Elisabeth; Lasbleiz, Christelle; Mignotte, Bernard; Renaud, Flore

2017-01-01

Neuroblastoma, a sympathetic nervous system tumor, accounts for 15% of cancer deaths in children. In contrast to most human tumors, p53 is rarely mutated in human primary neuroblastoma, suggesting impaired p53 activation in neuroblastoma. Various studies have shown correlations between fgf1 expression levels and both prognosis severity and tumor chemoresistance. As we previously showed that fibroblast growth factor 1 (FGF1) inhibited p53-dependent apoptosis in neuron-like PC12 cells, we initiated the study of the interaction between the FGF1 and p53 pathways in neuroblastoma. We focused on the activity of either extracellular FGF1 by adding recombinant rFGF1 in media, or of intracellular FGF1 by overexpression in human SH-SY5Y and mouse N2a neuroblastoma cell lines. In both cell lines, the genotoxic drug etoposide induced a classical mitochondrial p53-dependent apoptosis. FGF1 was able to inhibit p53-dependent apoptosis upstream of mitochondrial events in SH-SY5Y cells by both extracellular and intracellular pathways. Both rFGF1 addition and etoposide treatment increased fgf1 expression in SH-SY5Y cells. Conversely, rFGF1 or overexpressed FGF1 had no effect on p53-dependent apoptosis and fgf1 expression in neuroblastoma N2a cells. Using different FGF1 mutants (that is, FGF1K132E, FGF1S130A and FGF1S130D), we further showed that the C-terminal domain and phosphorylation of FGF1 regulate its intracrine anti-apoptotic activity in neuroblastoma SH-SY5Y cells. This study provides the first evidence for a role of an intracrine growth factor pathway on p53-dependent apoptosis in neuroblastoma, and could lead to the identification of key regulators involved in neuroblastoma tumor progression and chemoresistance. PMID:29048426

SNJ-1945, a calpain inhibitor, protects SH-SY5Y cells against MPP+ and rotenone

OpenAIRE

Knaryan, Varduhi H.; Samantaray, Supriti; Sookyoung, Park; Azuma, Mitsuyoshi; Inoue, Jun; Banik, Naren L.

2013-01-01

Complex pathophysiology of Parkinson’s disease (PD) involves multiple CNS cell types. Degeneration in spinal cord neurons alongside brain has been shown to be involved in PD and evidenced in experimental parkinsonism. However, the mechanisms of these degenerative pathways are not well understood. In order to unravel these mechanisms SH-SY5Y neuroblastoma cells were differentiated into dopaminergic and cholinergic phenotypes respectively and used as cell culture model following exposure to two...

Detection and localisation of the abalone probiotic Vibrio midae SY9 and its extracellular protease, VmproA, within the digestive tract of the South African abalone, Haliotis midae.

Directory of Open Access Journals (Sweden)

Robert J Huddy

Full Text Available Probiotics have been widely reported to increase the growth rate of commercially important fish and shellfish by enhancing the digestion of ingested feed through the production of extracellular enzymes such as proteases and alginases. In order to investigate this further, the objective of this study was to localise the bacterial probiont Vibrio midae SY9 and one of the extracellular proteases it produces in the digestive tract of the South African abalone Haliotis midae. This was accomplished by inserting a promotorless gfp gene into the chromosome of the bacterium which was incorporated in an artificial, fishmeal-based abalone feed. In situ histological comparison of abalone fed either a basal diet or the basal diet supplemented with V. midae SY9::Tn10.52 using a cocktail of DNA probes to the gfp gene localised the probiont to the crop/stomach and intestinal regions of the H. midae digestive tract. Generally, the ingested probiotic bacterium occurred in association with feed and particulate matter within the crop/stomach and intestinal regions, as well as adhered to the wall of the crop/stomach. Histological immunohistochemical examination using polyclonal anti-VmproA antibodies localised an extracellular protease produced by V. midae SY9 to the H. midae crop/stomach and intestine where it appeared to be associated with feed and/or other particulate matter in the abalone gut. Thus the data suggests that V. midae SY9 colonises and/or adheres to the mucous lining of the abalone gut. Furthermore, the close association observed between the bacterium, its extracellular protease and ingested feed particles supports the theory that V. midae SY9 elevates in situ digestive enzyme levels and thus enhances feed digestion in farmed abalone.

Detection and localisation of the abalone probiotic Vibrio midae SY9 and its extracellular protease, VmproA, within the digestive tract of the South African abalone, Haliotis midae.

Science.gov (United States)

Huddy, Robert J; Coyne, Vernon E

2014-01-01

Probiotics have been widely reported to increase the growth rate of commercially important fish and shellfish by enhancing the digestion of ingested feed through the production of extracellular enzymes such as proteases and alginases. In order to investigate this further, the objective of this study was to localise the bacterial probiont Vibrio midae SY9 and one of the extracellular proteases it produces in the digestive tract of the South African abalone Haliotis midae. This was accomplished by inserting a promotorless gfp gene into the chromosome of the bacterium which was incorporated in an artificial, fishmeal-based abalone feed. In situ histological comparison of abalone fed either a basal diet or the basal diet supplemented with V. midae SY9::Tn10.52 using a cocktail of DNA probes to the gfp gene localised the probiont to the crop/stomach and intestinal regions of the H. midae digestive tract. Generally, the ingested probiotic bacterium occurred in association with feed and particulate matter within the crop/stomach and intestinal regions, as well as adhered to the wall of the crop/stomach. Histological immunohistochemical examination using polyclonal anti-VmproA antibodies localised an extracellular protease produced by V. midae SY9 to the H. midae crop/stomach and intestine where it appeared to be associated with feed and/or other particulate matter in the abalone gut. Thus the data suggests that V. midae SY9 colonises and/or adheres to the mucous lining of the abalone gut. Furthermore, the close association observed between the bacterium, its extracellular protease and ingested feed particles supports the theory that V. midae SY9 elevates in situ digestive enzyme levels and thus enhances feed digestion in farmed abalone.

Optimization of cultivation conditions for biotechnological production of lipid by Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89 for biodiesel preparation

NARCIS (Netherlands)

Jiru, Tamene Milkessa; Groenewald, Marizeth; Pohl, Carolina; Steyn, Laurinda; Kiggundu, Nicholas; Abate, Dawit

Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89, an oleaginous yeast, isolated from Ethiopian soil, was grown under nitrogen-limited media. The capacity this with respect to biomass production, lipid yield and lipid content was evaluated. The influence of inoculum size, carbon

Do healthy school meals affect illness, allergies and school attendance in 8- to 11-year-old children?

DEFF Research Database (Denmark)

Laursen, Rikke Pilmann; Lauritzen, Lotte; Ritz, Christian

2015-01-01

Background/Objectives:A nutritionally adequate diet in childhood is important for health and resistance of allergies and infections. This study explored the effects of school meals rich in fish, vegetables and fibre on school attendance, asthma, allergies and illness in 797 Danish 8- to 11-year-o...

EXTENDED SCHOOL YEAR DESIGNS--AN INTRODUCTION TO NEW PLANS OF SCHOOL ORGANIZATION WHICH CAN RESULT IN FINANCIAL ECONOMIES AND PROVIDE MORE EDUCATION FOR ALL PUPILS.

Science.gov (United States)

THOMAS, GEORGE ISAIAH

THIS REPORT EXPLORES PLANS OF ELEMENTARY AND SECONDARY SCHOOL ORGANIZATION BASED UPON THE LENGTHENING OF THE SCHOOL YEAR TO REDUCE BY ONE OR MORE YEARS THE TOTAL PERIOD OF SCHOOLING. PRACTICAL INFORMATION IS PRESENTED CONCERNING (1) THE CONTINUOUS SCHOOL YEAR PLAN, (2) THE TRIMESTER PLAN, (3) THE QUADRIMESTER PLAN, (4) THE MODIFIED SUMMER SCHOOL…

The Komsomol and the Schools in the Lenin Year

Science.gov (United States)

Kutsenko, T.

1970-01-01

Describes the cooperative relationship between the Komsomol (All-Union Leninist Communist League of Youth), the Pioneers, and the schools, with specific reference to their mutual responsibilities in the Lenin Year. (JB)

Cordyceps sinensis Oral Liquid Inhibits Damage Induced by Oxygen and Glucose Deprivation in SH-SY5Y Cells.

Science.gov (United States)

Zou, Ying-Xin; Liu, Yu-Xiang; Ruan, Ming-Hua; Zhou, Yi; Wang, Jia-Chun; Chu, Zhi-Yong

2016-01-01

Cordyceps sinensis has been used in traditional Chinese medicine for thousands of years. It has been demonstrated to have a variety of biological activities, and an extract of it has been demonstrated to possess a protective effect in occlusion-induced focal cerebral ischemia of the middle cerebral artery in rats. It could be explored as an agent for treatment of ischemic stroke, and the mechanisms need to be studied further. The study intended to investigate the protective effects of the Cordyceps sinensis oral liquid (CSOL) against damage induced by oxygen and glucose deprivation (OGD) in SH-SY5Y cells. DESIGN • The research team designed an in vitro study. The study occurred at the Naval Medical Research Institute in Shanghai, China. SH-SY5Y cells were exposed to CSOL in doses of 0.01, 0.03, 0.10, 0.30, and 1.00 mg/mL, creating 5 intervention groups. The OGD condition was induced by transfer of the cells from high-glucose Dulbecco's Modified Eagle's medium (DMEM) in a box gassed with air containing 5% CO2 to glucose-free DMEM in a box gassed with 94% N2, 5% CO2, and 1% O2. Like the cells for the interventions groups, the cells for a model group were cultured with high-glucose DMEM and were transferred to the OGD, but they received no dose of COSL. Cells in a control group were cultured with high-glucose DMEM, were not transferred to the OGD condition, and did not receive any dose of COSL. Cell viability was assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The apoptosis and the mitochondrial membrane potential (MMP) were detected by flow cytometry, and the protein expression of caspase-3 was observed by western blot. After exposure to OGD, the cell viability of cells treated with 0.01, 0.03, 0.10, 0.30, and 1.00 mg/mL of CSOL increased in a dose-effect relationship. Compared with the cells in the model group, the treatment of CSOL at all the experimental concentrations significantly inhibited both the cell apoptosis

Assessment of dental caries predictors in 6-year-old school children - results from 5-year retrospective cohort study

Directory of Open Access Journals (Sweden)

Masood Mohd

2012-11-01

Full Text Available Abstract Background This was a retrospective cohort study undertaken to assess the rate and pattern of dental caries development in 6-year-old school children followed-up for a period of 5 years, and to identify baseline risk factors that were associated with 5 years caries experience in Malaysian children. Methods This 5-years retrospective cohort study comprised primary school children initially aged 6 years in 2004. Caries experience of each child was recorded annually using World Health Organization criteria. The rates of dental caries were recorded in prevalence and incidence density of carious lesions from baseline to final examination. Risk assessment was done to assess relative risk for caries after 5 years in children with baseline caries status. Simple and multiple logistic regression analysis were performed to identify significant independent risk factors for caries. Results The sample consisted of 1830 school children. All components of DMFT showed significant differences between baseline and final examination. Filled teeth (FT component of the DMFT showed the greatest increases. Results revealed the initial baseline caries level in permanent dentition was a strong predictor for future caries after 5 years (RR=3.78, 95% CI=3.48-4.10, P0.001. Logistic regression analysis showed significant association between caries occurrence and residence (urban/rural (OR=1.80, Pp observed from baseline and after 5 years was 5.80 persons/100 person-year of observation. The rate of new caries-affected tooth (IDt in the period from baseline and after 5-years was 0.76 teeth/100 teeth-year of observation. Conclusion The majority of 12-year-old school children (70% were caries-free and most of the caries were concentrated in only a small proportion (30% of them. We found that the presence of caries in permanent teeth at the age of 6 years was a strong predictor of future caries development in this population. The strong evidence of early permanent teeth

Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells

Energy Technology Data Exchange (ETDEWEB)

Zhang, L.; Liang, H. [Department of Pediatrics, Qilu Hospital, Shandong University, Jinan (China); Cao, W. [Department of Obstetrics, Qingdao Central Hospital, Qingdao (China); Xu, R.; Ju, X.L. [Department of Pediatrics, Qilu Hospital, Shandong University, Jinan (China)

2014-05-23

Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA interference on cell proliferation and invasion in vitro and in vivo. RNA interference of survivin led to a significant decrease in invasiveness and proliferation and increased apoptosis in SH-SY5Y cells in vitro. RNA interference of survivin inhibited tumor growth in vivo by 68±13% (P=0.002) and increased the number of apoptotic cells by 9.8±1.2% (P=0.001) compared with negative small interfering RNA (siRNA) treatment controls. Moreover, RNA interference of survivin inhibited the formation of lung metastases by 92% (P=0.002) and reduced microvascular density by 60% (P=0.0003). Survivin siRNA resulted in significant downregulation of survivin mRNA and protein expression both in vitro and in vivo compared with negative siRNA treatment controls. RNA interference of survivin was found to be a potent inhibitor of SH-SY5Y tumor growth and metastasis formation. These results support further clinical development of RNA interference of survivin as a treatment of neuroblastoma and other cancer types.

Baicalein antagonizes rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to Parkinsonism

OpenAIRE

Song Ju-Xian; Choi Mandy; Wong Kavin; Chung Winkie; Sze Stephen; Ng Tzi-Bun; Zhang Kalin

2012-01-01

Abstract Background Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism. Methods Cell viability and cytotoxicity were determined by MTT assay. The degree of nuclear apoptosis was evaluated with a fluorescent DNA-bindi...

DeSyGNER: A Building Block Architecture Fostering Independent Cooperative Development of Multimedia Knowledge Management Applications

OpenAIRE

Deibel, Stephan R.A.; Greenes, Robert A.; Snydr-Michal, Jan T.

1990-01-01

Multimedia knowledge management requires generalized sharing, composition and inter-relation of disparate data and knowledge elements. Since traditional computer operating systems treat programs as bodies of isolated functionality, inter-program connections can be made only by considerable effort on the part of each program author. We have been developing a multimedia kernel called DeSyGNER (the Decision Systems Group Nucleus of Extensible Resources) that provides basic multimedia knowledge m...

Evaluation of the Family/School Partnership Project, Year III, 1996-97.

Science.gov (United States)

Kester, Donald L.; Plakos, John; Santos, Will

The report details the third-year evaluation of a transitional bilingual education project at Chester W. Nimitz Middle School in Huntington Park, California. The Family/School Partnership Project is designed to assist limited English proficient students in mastering English skills to transition to regular classroom activities by integrating…

N-acetylaspartate (NAA) induces neuronal differentiation of SH-SY5Y neuroblastoma cell line and sensitizes it to chemotherapeutic agents.

Science.gov (United States)

Mazzoccoli, Carmela; Ruggieri, Vitalba; Tataranni, Tiziana; Agriesti, Francesca; Laurenzana, Ilaria; Fratello, Angelo; Capitanio, Nazzareno; Piccoli, Claudia

2016-05-03

Neuroblastoma is the most commonly extra-cranial solid tumor of childhood frequently diagnosed. The nervous system-specific metabolite N-acetylaspartate (NAA) is synthesized from aspartate and acetyl-CoA in neurons, it is among the most abundant metabolites present in the central nervous system (CNS) and appears to be involved in many CNS disorders. The functional significance of the high NAA concentration in the brain remains uncertain, but it confers to NAA a unique clinical significance exploited in magnetic resonance spectroscopy. In the current study, we show that treatment of SH-SY5Y neuroblastoma-derived cell line with sub-cytotoxic physiological concentrations of NAA inhibits cell growth. This effect is partly due to enhanced apoptosis, shown by decrease of the anti-apoptotic factors survivin and Bcl-xL, and partly to arrest of the cell-cycle progression, linked to enhanced expression of the cyclin-inhibitors p53, p21Cip1/Waf1 and p27Kip1. Moreover, NAA-treated SH-SY5Y cells exhibited morphological changes accompanied with increase of the neurogenic markers TH and MAP2 and down-regulation of the pluripotency markers OCT4 and CXCR4/CD184. Finally, NAA-pre-treated SH-SY5Y cells resulted more sensitive to the cytotoxic effect of the chemotherapeutic drugs Cisplatin and 5-fluorouracil.To our knowledge, this is the first study demonstrating the neuronal differentiating effects of NAA in neuroblastoma cells. NAA may be a potential preconditioning or adjuvant compound in chemotherapeutic treatment.

Special Education: A Statistical Report for the 1982-1983 School Year and the 1980-1981 and 1981-1982 School Years.

Science.gov (United States)

Molenaar, Marie

The two reports compiled here are intended as aids in evaluating and planning future educational efforts for handicapped students in New Jersey. The reports include a census of handicapped pupils, their programs, the service delivery personnel and special education due process cases for the 1980-81, 1981-82, and 1982-83 school years. Each report…

Factors affecting Mathematics achievement of first-year secondary school students in Central Uganda

OpenAIRE

Kiwanuka, Henry Nsubuga; Van Damme, Jan; Van Den Noortgate, Wim; Anumendem, Dickson Nkafu; Namusisi, Speranza

2015-01-01

This study explores the sources of variability in Mathematics achievement of Ugandan students at the student, classroom and school level. The Mathematics score and questionnaire responses of 4,819 first-year secondary school students (Grade Seven, about 14-15 years old) from 78 classrooms of 49 schools were analysed. A three-level linear model was used. The results indicate that out of the total variance in Mathematics achievement 68.8%, 14.2% and 17.0% are situated at student, classroom and ...

Math Anxiety and Math Ability in Early Primary School Years

Science.gov (United States)

Krinzinger, Helga; Kaufmann, Liane; Willmes, Klaus

2010-01-01

Mathematical learning disabilities (MLDs) are often associated with math anxiety, yet until now, very little is known about the causal relations between calculation ability and math anxiety during early primary school years. The main aim of this study was to longitudinally investigate the relationship between calculation ability, self-reported evaluation of mathematics, and math anxiety in 140 primary school children between the end of first grade and the middle of third grade. Structural equation modeling revealed a strong influence of calculation ability and math anxiety on the evaluation of mathematics but no effect of math anxiety on calculation ability or vice versa—contrasting with the frequent clinical reports of math anxiety even in very young MLD children. To summarize, our study is a first step toward a better understanding of the link between math anxiety and math performance in early primary school years performance during typical and atypical courses of development. PMID:20401159

A school-randomized clinical trial of an integrated social-emotional learning and literacy intervention: impacts after 1 school year.

Science.gov (United States)

Jones, Stephanie M; Brown, Joshua L; Hoglund, Wendy L G; Aber, J Lawrence

2010-12-01

To report experimental impacts of a universal, integrated school-based intervention in social-emotional learning and literacy development on change over 1 school year in 3rd-grade children's social-emotional, behavioral, and academic outcomes. This study employed a school-randomized, experimental design and included 942 3rd-grade children (49% boys; 45.6% Hispanic/Latino, 41.1% Black/African American, 4.7% non-Hispanic White, and 8.6% other racial/ethnic groups, including Asian, Pacific Islander, Native American) in 18 New York City public elementary schools. Data on children's social-cognitive processes (e.g., hostile attribution biases), behavioral symptomatology (e.g., conduct problems), and literacy skills and academic achievement (e.g., reading achievement) were collected in the fall and spring of 1 school year. There were main effects of the 4Rs Program after 1 year on only 2 of the 13 outcomes examined. These include children's self-reports of hostile attributional biases (Cohen's d = 0.20) and depression (d = 0.24). As expected based on program and developmental theory, there were impacts of the intervention for those children identified by teachers at baseline with the highest levels of aggression (d = 0.32-0.59) on 4 other outcomes: children's self-reports of aggressive fantasies, teacher reports of academic skills, reading achievement scaled scores, and children's attendance. This report of effects of the 4Rs intervention on individual children across domains of functioning after 1 school year represents an important first step in establishing a better understanding of what is achievable by a schoolwide intervention such as the 4Rs in its earliest stages of unfolding. The first-year impacts, combined with our knowledge of sustained and expanded effects after a second year, provide evidence that this intervention may be initiating positive developmental cascades both in the general population of students and among those at highest behavioral risk. (Psyc

Postural education program for elementary school: a one-year follow-up study

Directory of Open Access Journals (Sweden)

Alexandre Luis Ritter

2015-09-01

Full Text Available AbstractThe objective of this study was to verify the short- and long-term effectiveness of the Elementary School Postural Program in the performance, generalization, and perception of daily school activities. The final sample consisted of 61 subjects divided into experimental (14 years ±0.93; ♀=22; ♂=10 and control group (15.38 years ±0.97; ♀=16; ♂=13, all participants attended a Brazilian public school in Porto Alegre, State of Rio Grande do Sul. The postural program included 20 sessions over a 10-week period. In each session, participants discussed and practiced routine actions that typically occurred at schools. Eight other meetings were required for the completion of the pre- and post-tests. The experimental group performed significantly better than the control group in the short-term evaluations, although there were no significant statistical differences in the long-term follow-up evaluations. The children demonstrated a good behavioral response to the postural program; nevertheless, the knowledge had not been completely mastered after a year.

School Nurses Race to the Top: The Pilot Year of How One District's School Nurses Revised Their Evaluation Process

Science.gov (United States)

Haffke, Louise Marie; Damm, Paula; Cross, Barbara

2014-01-01

During the 2013-2014 school year, the Shaker Heights, Ohio City school district was mandated to change its evaluation process as part of the Race to the Top initiative. Although not required by the federal or state Departments of Education, the Shaker Heights City school district tasked all members of their faculty and staff, including school…

34 CFR 300.106 - Extended school year services.

Science.gov (United States)

2010-07-01

... services means special education and related services that— (1) Are provided to a child with a disability... 34 Education 2 2010-07-01 2010-07-01 false Extended school year services. 300.106 Section 300.106 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION...

Paracrine Maturation and Migration of SH-SY5Y Cells by Dental Pulp Stem Cells

OpenAIRE

Gervois, Pascal; Wolfs, Esther; Dillen, Yörg; Hilkens, Petra; Ratajczak, Jessica; Driesen, Ronald; Vangansewinkel, Tim; Bronckaers, Annelies; Brône, Bert; Struys, Tom; Lambrichts, Ivo

2017-01-01

Neurological disorders are characterized by neurodegeneration and/or loss of neuronal function, which cannot be adequately repaired by the host. Therefore, there is need for novel treatment options such as cell-based therapies that aim to salvage or reconstitute the lost tissue or that stimulate host repair. The present study aimed to evaluate the paracrine effects of human dental pulp stem cells (hDPSCs) on the migration and neural maturation of human SH-SY5Y neuroblastoma cells. The hDPSC s...

Accuracy of 11-year-olds selfreported school lunch consumption

DEFF Research Database (Denmark)

Lyng, Nina

accuracy differ by the lunch format consumed (Paper III) Material and methods The study was conducted as a cross-sectional dietary reporting study. The population consisted of 11-year-old children from three public schools in Copenhagen. The study was conducted on two consecutive days and assessed...... in general. Objectives The aim of the present thesis was to assess food level reporting accuracy in Danish 11-year-old children’s self-reported school lunch consumption, and the aim was operationalized in following objectives. 1- To identify food items clustering by lunch format (Preliminary analyses) 2......- To assess reporting accuracy in relation to gender and self-reported methods (Paper I) 3- To address aspects of reporting inaccuracy from intrusions by food group, against different objective measures, and classification of intrusions in stretches and confabulations (Paper II) 4- To assess how reporting...

How wide is the gap between high school and first-year chemistry at ...

African Journals Online (AJOL)

The aim of the study was to identify the nature and extent of the gap between high school and first-year chemistry at the University of the Witwatersrand. The investigation was done at the macro and micro levels. At the macro level high school physical science and first-year chemistry syllabuses were compared. The testing ...

The First-Year Urban High School Teacher: Holding the Torch, Lighting the Fire

Science.gov (United States)

Weinberg, Paul J.; Weinberg, Carl

2008-01-01

The book tracks co-author Paul Weinberg during his first year of teaching as he is introduced to the daily tribulations of an urban Los Angeles high school. Paul's father Carl Weinberg, who fifty years earlier himself began his career in education an urban secondary school teacher, shares his experiences side-by-side with those of his son.…

Grade 10 PSAT Participation and Performance--School Year 2015-2016. Memorandum

Science.gov (United States)

Navarro, Maria V.

2016-01-01

The 2015-2016 school year marks the first administration of the redesigned PSAT, which is composed of two sections: (1) Evidence-based Reading and Writing (EBRW) and (2) Math. This memorandum presents results of 2015-2016 Grade 10 PSAT participation and performance of Montgomery County Public Schools (MCPS) students. Among 10,859 MCPS first-time…

Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP+

Directory of Open Access Journals (Sweden)

Kawinthra Khwanraj

2015-01-01

Full Text Available The human neuroblastoma SH-SY5Y cell line has been used as a dopaminergic cell model for Parkinson’s disease research. Whether undifferentiated or differentiated SH-SY5Y cells are more suitable remains controversial. This study aims to evaluate the expression of apoptosis-related mRNAs activated by MPP+ and evaluate the differential expression of tyrosine hydroxylase (TH in undifferentiated and retinoic acid- (RA- induced differentiated cells. The western blot results showed a gradual decrease in TH in undifferentiated cells and a gradual increase in TH in differentiated cells from days 4 to 10 after cell plating. Immunostaining revealed a gradual increase in TH along with neuritic outgrowth in differentiated cells on days 4 and 7 of RA treatment. For the study on cell susceptibility to MPP+ and the expression of apoptosis-related genes, MTT assay showed a decrease in cell viability to approximately 50% requiring 500 and 1000 μM of MPP+ for undifferentiated and RA-differentiated cells, respectively. Using real-time RT-PCR, treatment with 500 μM MPP+ led to significant increases in the Bax/Bcl-2 ratio, p53, and caspase-3 in undifferentiated cells but was without significance in differentiated cells. In conclusion, differentiated cells may be more suitable, and the shorter duration of RA differentiation may make the SH-SY5Y cell model more accessible.

A CTE Legacy Built on Chocolate: Milton Hershey School's 100 Years

Science.gov (United States)

Kemmery, Robert

2010-01-01

One hundred years ago, Chocolate Magnate Milton S. Hershey and his wife Catherine signed the deed of trust creating the Hershey Industrial School in the heart of their Pennsylvania farming community. They had no children of their own and wanted to help orphan boys get a good education. The couple eventually left their entire fortune to the school.…

Project A+, Elementary Technology Demonstration Schools, 1991-92: The Second Year.

Science.gov (United States)

Nichols, Todd; Frazer, Linda

The Elementary Technology Demonstration Schools program, where four elementary schools were equipped with computer hardware and software, was made possible by grants from IBM and Apple, Inc. The goals of the program were, in 3 years, to reduce by 50% the number of students not in their age appropriate grade level and those students not achieving…

Child development at 5 years of age predicted mathematics ability and schooling outcomes in Malawian adolescents.

Science.gov (United States)

Gandhi, Mihir; Teivaanmaki, Tiina; Maleta, Kenneth; Duan, Xiaolian; Ashorn, Per; Cheung, Yin Bun

2013-01-01

This study aimed to examine the association between child development at 5 years of age and mathematics ability and schooling outcomes at 12 years of age in Malawian children. A prospective cohort study looking at 609 rural Malawian children. Outcome measures were percentage of correctly answered mathematics questions, highest school grade completed and number of times repeating school grades at 12 years of age. A child development summary score obtained at 5 years of age was the main exposure variable. Regression analyses were used to estimate the association and adjust for confounders. Sensitivity analysis was performed by handling losses to follow-up with multiple imputation (MI) method. The summary score was positively associated with percentage of correctly answered mathematics questions (p = 0.057; p = 0.031 MI) and with highest school grade completed (p = 0.096; p = 0.070 MI), and negatively associated with number of times repeating school grades (p = 0.834; p = 0.339 MI). Fine motor score at 5 years was independently associated with the mathematic score (p = 0.032; p = 0.011 MI). The association between child development and mathematics ability did not depend on school attendance. Child development at 5 years of age showed signs of positive association with mathematics ability and possibly with highest school grade completed at 12 years of age. © 2012 The Author(s)/Acta Paediatrica © 2012 Foundation Acta Paediatrica.

Effects of a 3-year intervention: The Copenhagen School Child Intervention Study

DEFF Research Database (Denmark)

Bugge, Anna; El-Naaman, Bianca; Dencker, Magnus

2012-01-01

INTRODUCTION: This study assessed short and long term effects of a 3-year controlled school-based physical activity (PA) intervention on fatness, cardiorespiratory fitness (VO2peak) and cardiovascular disease (CVD) risk factors in children. METHODS: The study involved 18 schools (10 intervention...

The Biochemistry Over 20 Years In The High School Textbooks

Directory of Open Access Journals (Sweden)

C. E.S. Rocha

2014-08-01

Full Text Available   The Biochemistry Over 20 Years In The High School Textbooks   Rocha, C. E. S.1; Büttenbender, M. D.1; Denardin, E.L.G.2, Roehrs, R.1,2 1Grupo Interdisciplinar de Pesquisa em Práticas de Ensino, UNIPAMPA, RS. 2Laboratório de Estudos Físico Químicos e Produtos Naturais, UNIPAMPA, RS.   INTRODUCTION: Many teachers make use of textbook to lead content in the classroom. The chemistry books introduce concepts that should relate biochemistry to students in schools. It is important that this first contact turns out into an encouraging experience for the students, because once it worked as expected it arouses interest and makes the students see themselves curious to delve into the subject. The research aims to evaluate the presence of related concepts in biochemistry textbooks in chemistry in high school, over 20 years. MATERIAL AND METHODS: In order to perform this study, we analyzed the following content related to biochemistry: proteins, carbohydrates, lipids and nucleic acids in the books "Chemistry - Structure of Matter and Organic Chemistry" of the year 1993 and the book "Chemistry in approach to daily life" of the year 2012 with the purpose of verifying the changes in the content of biochemistry in the last 20 years. RESULTS AND DISCUSSION: In the 90s, as used in the book, concepts and explanations are introduced in a very objective approach, making a total of 22 pages. The current largest is 23 pages with experiments and curiosities. Through analysis we found that current textbooks present the same issues related to biochemistry, however, a greater amount of data, bringing students to more examples and applications in everyday life. Today we see that the contents and processes are most exploited and that there is a concern on the importance of the study of issues that relate to biochemistry. CONCLUSIONS: The study of the biochemistry textbooks has been more attractive in recent years, contextualizing content with the daily life of

Modulation of intracellular calcium homeostasis by trimethyltin chloride in human tumour cells: Neuroblastoma SY5Y and cervix adenocarcinoma HeLa S3

International Nuclear Information System (INIS)

Florea, Ana-Maria; Splettstoesser, Frank; Dopp, Elke; Rettenmeier, Albert W.; Buesselberg, Dietrich

2005-01-01

Physiological modifications of intracellular Ca 2+ ([Ca 2+ ] i ) levels trigger and/or regulate a diversity of cellular activities (e.g. neurotransmitter release, synaptic plasticity, muscular contraction, cell proliferation), while calcium overloads could result in cytotoxicity. Previously, we have shown that trimethyltin chloride (Me 3 SnCl; TMT) modulates calcium homeostasis in cervix adenocarcinoma (HeLa S3) cells [Florea, A.-M., Dopp, E., Buesselberg, D., 2005. TMT induces elevated calcium transients in HeLa cells: types and levels of response. Cell Calcium 37, 252-258]. Here we compare [Ca 2+ ] i -changes induced by trimethyltin chloride in neuroblastoma SY5Y and HeLa S3 cells using calcium-sensitive dyes (fluo-4/AM (fluo-4) and rhod-2/AM (rhod-2)) and laser scanning microscopy (LSM). TMT-induced calcium elevations in neuroblastoma SY5Y as well as in HeLa S3 cells. [Ca 2+ ] i rose to a sustained plateau or to transient spikes. Overall, the detected averaged increase of the maximum calcium elevation were: 0.5 μM ∼125.6%; 5 μM ∼130.1%; 500 μM ∼145% in HeLa S3 cells and 0.5 μM ∼133.3%; 5 μM ∼136.1%; 500 μM ∼147.1% in neuroblastoma SY5Y cells. The calcium rise derived from internal stores did not significantly depend on the presence of calcium in the external solution: ∼109% (no calcium added) versus ∼117% (2 mM calcium; 5 μM TMT) in HeLa cells. This difference was similar in neuroblastoma SY5Y cells, were ∼127% versus ∼136% increase (5 μM TMT) were measured. Staining of calcium stores with rhod-2 showed a TMT-induced [Ca 2+ ] i -decrease in the stores followed by an increase of the calcium concentration in the nuclei of the two cell lines tested. Our results suggest that toxic effects in human tumour cells after exposure to trimethyltin compounds might be due to an elevation of [Ca 2+ ] i

Die trauma van tuiste en (niebehoort in Zimbabwe en sy diaspora: ‘Omsettingsversteuring’ in Shadows deur Novuyo Rosa Tshuma

Directory of Open Access Journals (Sweden)

Johan U. Jacobs

2016-08-01

Full Text Available Die hernude xenofobiese aanvalle in Maart en April 2015 in Durban, Johannesburg en Kaapstad, die roof, afbrand van huise en winkels, lewensverlies, en vlug van duisende vreemde Afrikane na toevlugskampe, het nie alleen die hele kwessie rondom die Afrika-diaspora in Suid-Afrika na vore gebring nie, maar ook die vraag oor die betekenis van die begrippe ‘tuiste’ en ‘tuisland’ binne ’n diasporiese verband. In Shadows, wat in 2014 met die Herman Charles Bosman-prys bekroon is, beeld die Zimbabwiese skryfster Novuyo Rosa Tshuma die ontwrigte lewensomstandighede in die hedendaagse Zimbabwe uit, asook die hervestiging en dubbele vervreemding van die diasporiese Zimbabwiër-gemeenskap in Suid-Afrika. Die teks bestaan uit ’n novelle, ‘Shadows’, en vyf ander kortverhale, en kan bes beskou word as ’n verhaalsiklus, waarbinne the afsonderlike verhale verbind word deur die tema van diaspora en ook deur ’n aantal herkenbare diasporiese situasies en beelde. Die artikel put uit ‘n reeks opvattings oor diaspora, insluitende die Afrika- en binne-Afrika diaspora, en huidige navorsing oor Zimbabwe en sy diaspora. Die dubbelsinnige begrippe van tuiste en samehorigheid, insluiting en uitsluiting, word ondersoek na aanleiding van Tshuma se uitbeelding van die alledaagse township-lewe in Mugabe se Zimbabwe met sy voedseltekorte, algemene gebrek aan middele, en vernielde ekonomie, en hierteenoor, die randbestaan van die hedendaagse Zimbabwediaspora in Johannesburg met sy gewelddadige vervolging en korrupte magsmisbruike teen onwettige immigrante. Die artikel stel voor dat die psigogeniese toestand van ‘omskakelingsversteuring’, wat Tshuma beklemtoon in een van haar verhale, as ’n metafoor kan dien vir die paradoksale diasporiese vereenselwiging met, en vervreemding van, ’n tuiste, gemeenskap en tuisland in Zimbabwe sowel as in die onvriendelike gasheerland, Suid-Afrika

School nurses' experiences of delivering the UK HPV vaccination programme in its first year.

Science.gov (United States)

Hilton, Shona; Hunt, Kate; Bedford, Helen; Petticrew, Mark

2011-08-24

In the United Kingdom (UK) in September 2008, school nurses began delivering the HPV immunisation programme for girls aged 12 and 13 years old. This study offers insights from school nurses' perspectives and experiences of delivering this new vaccination programme. Thirty in-depth telephone interviews were conducted with school nurses working across the UK between September 2008 and May 2009. This time period covers the first year of the HPV vaccination programme in schools. School nurses were recruited via GP practices, the internet and posters targeted at school nurse practitioners. All the school nurses spoke of readying themselves for a deluge of phone calls from concerned parents, but found that in fact few parents telephoned to ask for more information or express their concerns about the HPV vaccine. Several school nurses mentioned a lack of planning by policy makers and stated that at its introduction they felt ill prepared. The impact on school nurses' workload was spoken about at length by all the school nurses. They believed that the programme had vastly increased their workload leading them to cut back on their core activities and the time they could dedicate to offering support to vulnerable pupils. Overall the first year of the implementation of the HPV vaccination programme in the UK has exceeded school nurses' expectations and some of its success may be attributed to the school nurses' commitment to the programme. It is also the case that other factors, including positive newsprint media reporting that accompanied the introduction of the HPV vaccination programme may have played a role. Nevertheless, school nurses also believed that the programme had vastly increased their workload leading them to cut back on their core activities and as such they could no longer dedicate time to offer support to vulnerable pupils. This unintentional aspect of the programme may be worthy of further exploration.

Solubilities of gases in simulated Tank 241-SY-101 wastes

International Nuclear Information System (INIS)

Norton, J.D.; Pederson, L.R.

1995-09-01

Oxygen, nitrogen, hydrogen, methane, and nitrous oxide solubilities were evaluated as a function of temperature in SYl-SIM-93B, a homogeneous simulated waste mixture containing sodium hydroxide, sodium nitrite, sodium nitrate, sodium aluminate, and sodium carbonate, the principal inorganic constituents of the wastes in Tank 241-SY-101. Ammonia solubility data for this simulated waste was obtained as a function of temperature in an earlier study. The choice of a homogeneous waste mixture in this study has the advantage of eliminating complications associated with a changing electrolyte concentration as a function of temperature that would be encountered with a slurry simulant. Dissolution is one of the means by which gases may be retained in Hanford Site wastes. While models are available to estimate gas solubilities in electrolyte solutions, few data are in existence that pertain to highly concentrated, multicomponent electrolytes such as those stored in Hanford Site waste tanks

The anti-inflammatory effect of melatonin in SH-SY5Y neuroblastoma cells exposed to sublethal dose of hydrogen peroxide.

Science.gov (United States)

Nopparat, Chutikorn; Chantadul, Varunya; Permpoonputtana, Kannika; Govitrapong, Piyarat

2017-06-01

Brain inflammaging is considered as one of the underlying factors of neurodegenerative diseases. The present study aimed to investigate the effects of melatonin, an endogenous indoleamine mainly synthesized by the pineal gland, on hydrogen peroxide (H 2 O 2 )-induced inflammaging state in SH-SY5Y cells. Our data showed that p21 Cip1 and p16 INK4a , cell cycle arrest markers, and the number of senescence-associated β-galactosidase (SA-βgal) staining increased significantly in H 2 O 2 -treated cells. Melatonin treatment could reverse this effect. Flow cytometry analysis showed a significantly higher percentage in the G0/G1 phase and a lower proportion in the S phase of H 2 O 2 treated cells. Cells pretreated with H 2 O 2 showed a dramatic decrease in the formation of Ki67 immunoactivity while the treatment with melatonin increased Ki67-positive cell. Both mRNA and protein expression levels of the pro-inflammatory cytokines, interleukin-1β (IL-1β), IL-6 and, tumor necrosis factor-α (TNF-α) which were increased after induction with H 2 O 2 , could be attenuated by melatonin. In addition, melatonin decreased the phospho-nuclear factor kappa B (pNF-κB) expression and prevented its nuclear translocation, as well as abrogated the reduction of nuclear factor erythroid 2-related factor 2 (Nrf2) in SH-SY5Y cells exposed to H 2 O 2 . The present data suggested the importance of melatonin on ameliorating inflammation in SH-SY5Y cells. Copyright © 2017 Elsevier B.V. All rights reserved.

The behavior, quantity, and location of undissolved gas in Tank 241-SY-101

Energy Technology Data Exchange (ETDEWEB)

Brewster, M.E.; Gallagher, N.B.; Hudson, J.D.; Stewart, C.W.

1995-10-01

Mitigation of episodic flammable gas releases from Hanford Waste Tank 241-SY-101 was accomplished in July 1993 with the installation of a mixer pump that prevents gas retention. But is has not been possible until recently to measure the effects of mixing on the waste or how much gas remains and where it is located. Direct measurements of the void fraction and rheology of the mixed waste by the void fraction instrument (VFI) and ball rheometer along with previous data provide estimates of the location, quantity, and behavior of undissolved gas in the tank. This report documents the compilation and integration of the information that enables this understanding.

School year versus summer differences in child weight gain: A narrative review

Science.gov (United States)

The causes of the current high prevalence of overweight and obesity among children are not clearly known. Schools have been implicated in the causal chain to high child obesity prevalence. Recent studies have compared school year versus summertime changes (herein called seasonal differences) in chil...

Emerging Partnership Practices in VET Provision in the Senior Years of Schooling in Australia

Science.gov (United States)

Klatt, Gosia; Angelico, Teresa; Polesel, John

2018-01-01

School partnerships support the effective provision of Vocational Education and Training (VET) in the senior years of secondary schooling, to a varying degree, in most OECD nations. However, the nature and quality of these partnerships vary considerably from school to school and, indeed, from nation to nation (see Murray and Polesel, "Eur J…

Factors affecting Mathematics achievement of first-year secondary school students in Central Uganda

Directory of Open Access Journals (Sweden)

Henry Nsubuga Kiwanuka

2015-08-01

Full Text Available This study explores the sources of variability in Mathematics achievement of Ugandan students at the student, classroom and school level. The Mathematics score and questionnaire responses of 4,819 first-year secondary school students (Grade Seven, about 14-15 years old from 78 classrooms of 49 schools were analysed. A three-level linear model was used. The results indicate that out of the total variance in Mathematics achievement 68.8%, 14.2% and 17.0% are situated at student, classroom and school level, respectively. Of all the considered explanatory variables at the three levels, i.e. socio-economic status, gender, prior Mathematics achievement, parental support, peer influence, class mean of prior Mathematics achievement and of students' perception of good classroom assessment, school mean of class climate (class mean of attitude toward mathematics and of parental support were significant predictors of Mathematics achievement. The relevant factors could explain 7.6%, 73.1% and 84.3%, respectively, of student-, classroom- and school-level differences. Implications of our study are considered.

Cohort Graduation Rate: Policy and Technical Manual. 2016-17 Graduation Rates Based on Students First Entering High School during the 2012-13 and 2013-14 School Years

Science.gov (United States)

Oregon Department of Education, 2017

2017-01-01

High School graduation rates are key indicators of accountability for high schools and school districts in Oregon. Beginning with the 2008-09 school year, the Oregon Department of Education (ODE) implemented the cohort method of calculating graduation rates. The cohort method identifies the year the student entered high school for the first time…

Tracer Study of BS in Information Technology (BSIT Graduates of Camarines Sur Polytechnic Colleges, Nabua, Camarines Sur from 2004 to 2010

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ARLY B. BALINGBING

2014-08-01

Full Text Available Tracer studies enhance curriculum relevance and marketability of educational programs. Knowledge on employment of CSPC graduates assists in formulating policy towards combating unemployment and producing globally competitive BSIT graduates. This study determines the employability of BSIT graduates of SY 2004-2010 and unveil specifically their personal profile, level of competency alongknowledge, skills and attitudes, significant relationship of the level of competency and the level of difficulties encountered along knowledge, skills, and attitudes and the measures to enhance their competitiveness. The study used descriptive method. Data gathered through a questionnaire from 155 respondents were statistically treated using averaging, percentage, weighted mean and Spearman Rho. Most respondents were female, 20-25 years old, TESDA-certified, employed in private sectors, incompetent in SY 2003-2004, competent in SY 2005-2010and encountered difficulty along knowledge, skills, and attitudes. There is no significant relationship in the level of competencies among graduates per school year and theirlevel of difficulty along knowledge, skills and attitudes. Some measures to enhance their competitivenessinclude conducting TESDA Assessment and Civil Service examination review and IT Faculty in-house trainings and strengthening English Proficiency Program. BSIT graduates of SY 2004-2010 are competent despite difficulties encountered along skills, attitudes and knowledge.

Bullying victimization among 13 til 15 year old school children

DEFF Research Database (Denmark)

Due, Pernille; Holstein, Bjørn Evald

2008-01-01

AIM: to examine the prevalence of bullying victimization in 66 countries and territories from five continents based on data from two large international surveys: the 2001/2 Health Behavior in School-aged Children survey (HBSC) and the Global School-based Students Health Survey (GSHS). The surveys...... provide nationally representative, cross-sectional information on 13-15-year-old school children (N = 218,104). OUTCOME MEASURES: Bullying victimization, once or more within the past 2 months (HBSC)/30 days (GSHS). RESULTS: On average, 32.1% of the children were bullied at school at least once within...... the past 2 months in countries involved in the HBSC study and 37.4% of children were bullied at least one day within the past 30 days in countries involved in the GSHS study. In both surveys, a large variation in prevalence was found across countries. The lowest prevalence in the GSHS survey was observed...

Prevalence of Sport Specialization in High School Athletics: A 1-Year Observational Study.

Science.gov (United States)

Bell, David R; Post, Eric G; Trigsted, Stephanie M; Hetzel, Scott; McGuine, Timothy A; Brooks, M Alison

2016-06-01

The prevalence of sport specialization in high school athletes is unknown. This information is needed to determine the scope of this issue in an active population. To determine the prevalence of sport specialization in high school athletes and to determine if specialization is influenced by classification method, year in school, sex, and school size. A secondary purpose was to determine if highly specialized athletes would be more likely to report a history of lower extremity injuries. Cross-sectional study; Level of evidence, 3. High school athletes between the ages of 13 and 18 years from 2 local high schools completed both a sport specialization survey and an injury history survey. Athletes were classified into low, moderate, or high specialization groups using a recently developed 3-point system and were also classified using a self-classification method. A total of 302 athletes completed the surveys and were classified as low specialization (n = 105, 34.8%), moderate specialization (n = 87, 28.8%), or high specialization (n = 110, 36.4%). Athletes from the small school were more likely to be classified in the low specialization group (low, 43%; moderate, 32%; high, 25%) compared with those from the large school (low, 26%; moderate, 26%; high, 48%) (P single sport (n = 89, 29.5%). Athletes from the small school were more likely to classify themselves as multisport (n = 128, 86%) (P school (n = 85, 56%). There were no differences in the history of hip, knee, or ankle injuries between athletes who self-classified as single sport (hip: n = 10, 3%; knee: n = 19, 6%; ankle: n = 35, 12%) versus those who self-classified as multisport (hip: n = 45, 8%; knee: n = 23, 15%; ankle: n = 98, 33%) (P > .370). Classification method and school size influenced the prevalence of specialization in high school athletes. Highly specialized athletes were more likely to report a history of overuse knee or hip injuries. Participating in a single sport for more than 8 months per year

Administrative Problems in the Single-Track Year-Round High Schools: Research Findings and Guidelines.

Science.gov (United States)

Holt, Laura L.; Karr-Kidwell, PJ

An analysis of the problems pertaining to the adoption of a year-round calendar for high schools, along with the advantages of year-round education (YRE), are examined. It provides a literary review (including historical contexts), types of calendars, benefits, administrative problems, and societal benefits. For the study, 28 schools responded to…

Evaluating the Promise of Single-Track Year-Round Schools.

Science.gov (United States)

Haenn, Joseph F.

1996-01-01

Describes two single-track year-round elementary schools in Durham, North Carolina, established in discrete attendance zones. Remediation and enrichment activities were provided during intersession. Low-SES students were overrepresented in remediation sessions. Student outcomes data (end-of-grade reading and math test scores) suggest that…

Changes in diet from age 10 to 14 years and prospective associations with school lunch choice.

Science.gov (United States)

Winpenny, Eleanor M; Corder, Kirsten L; Jones, Andy; Ambrosini, Gina L; White, Martin; van Sluijs, Esther M F

2017-09-01

There is limited evidence on how diet changes over the transition from primary to secondary school. In this study we investigated changes in diet from age 10 (2007) to age 14 years (2011) and the contribution of school-time consumption and school lunch choice to such changes. The 351 participants with dietary data (4 day food record) available at baseline (age 10 years) and follow-up (age 14 years) were included. Multi-level regression models were fitted for absolute or change in food and nutrient intake, cross-classified by primary and secondary school attended as appropriate, with adjustment for covariates and mis-reporting. From age 10 to age 14 years, children decreased energy intake from sugars (-2.6% energy (%E)) (standard error (SE) 0.44) and from saturated fats (-0.54%E (SE 0.18)), decreased fruit (-3.13 g/MJ (SE 1.04)) and vegetables (-1.55 g/MJ (SE 0.46)) consumption and increased sugar sweetened beverage (SSB) (4.66  g/MJ (SE 1.87)) and fries (1.31  g/MJ (SE 0.39)) consumption. Intake of snack foods, SSBs, and fries, but also fruits and vegetables was higher outside school hours. Prospective change from non-school lunch to school lunch, compared to maintaining non-school lunch consumption, was associated with decreased consumption of savoury snacks (-8.32 g/day (SE 2.03)), increased consumption of fries (12.8 g/day (SE 4.01)) and decreased consumption of fruit (-25.16 g/day (SE 11.02)) during school hours. Changes in diet from age 10 to age 14 years differed within and outside of school hours. Consumption of a school lunch, compared to lunch obtained elsewhere, was associated with negative as well as positive changes in diet, suggesting that any efforts to encourage school lunch take-up need to be accompanied by further efforts to improve school lunch provision to meet nutritional guidelines. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

School nurses' experiences of delivering the UK HPV vaccination programme in its first year

Science.gov (United States)

2011-01-01

Background In the United Kingdom (UK) in September 2008, school nurses began delivering the HPV immunisation programme for girls aged 12 and 13 years old. This study offers insights from school nurses' perspectives and experiences of delivering this new vaccination programme. Methods Thirty in-depth telephone interviews were conducted with school nurses working across the UK between September 2008 and May 2009. This time period covers the first year of the HPV vaccination programme in schools. School nurses were recruited via GP practices, the internet and posters targeted at school nurse practitioners. Results All the school nurses spoke of readying themselves for a deluge of phone calls from concerned parents, but found that in fact few parents telephoned to ask for more information or express their concerns about the HPV vaccine. Several school nurses mentioned a lack of planning by policy makers and stated that at its introduction they felt ill prepared. The impact on school nurses' workload was spoken about at length by all the school nurses. They believed that the programme had vastly increased their workload leading them to cut back on their core activities and the time they could dedicate to offering support to vulnerable pupils. Conclusion Overall the first year of the implementation of the HPV vaccination programme in the UK has exceeded school nurses' expectations and some of its success may be attributed to the school nurses' commitment to the programme. It is also the case that other factors, including positive newsprint media reporting that accompanied the introduction of the HPV vaccination programme may have played a role. Nevertheless, school nurses also believed that the programme had vastly increased their workload leading them to cut back on their core activities and as such they could no longer dedicate time to offer support to vulnerable pupils. This unintentional aspect of the programme may be worthy of further exploration. PMID:21864404

School nurses' experiences of delivering the UK HPV vaccination programme in its first year

Directory of Open Access Journals (Sweden)

Bedford Helen

2011-08-01

Full Text Available Abstract Background In the United Kingdom (UK in September 2008, school nurses began delivering the HPV immunisation programme for girls aged 12 and 13 years old. This study offers insights from school nurses' perspectives and experiences of delivering this new vaccination programme. Methods Thirty in-depth telephone interviews were conducted with school nurses working across the UK between September 2008 and May 2009. This time period covers the first year of the HPV vaccination programme in schools. School nurses were recruited via GP practices, the internet and posters targeted at school nurse practitioners. Results All the school nurses spoke of readying themselves for a deluge of phone calls from concerned parents, but found that in fact few parents telephoned to ask for more information or express their concerns about the HPV vaccine. Several school nurses mentioned a lack of planning by policy makers and stated that at its introduction they felt ill prepared. The impact on school nurses' workload was spoken about at length by all the school nurses. They believed that the programme had vastly increased their workload leading them to cut back on their core activities and the time they could dedicate to offering support to vulnerable pupils. Conclusion Overall the first year of the implementation of the HPV vaccination programme in the UK has exceeded school nurses' expectations and some of its success may be attributed to the school nurses' commitment to the programme. It is also the case that other factors, including positive newsprint media reporting that accompanied the introduction of the HPV vaccination programme may have played a role. Nevertheless, school nurses also believed that the programme had vastly increased their workload leading them to cut back on their core activities and as such they could no longer dedicate time to offer support to vulnerable pupils. This unintentional aspect of the programme may be worthy of further

An Involvement of PI3-K/Akt Activation and Inhibition of AIF Translocation in Neuroprotective Effects of Undecylenic Acid (UDA) Against Pro-Apoptotic Factors-Induced Cell Death in Human Neuroblastoma SH-SY5Y Cells.

Science.gov (United States)

Jantas, Danuta; Piotrowski, Marek; Lason, Wladyslaw

2015-12-01

Undecylenic acid (UDA), a naturally occurring 11-carbon unsaturated fatty acid, has been used for several years as an economical antifungal agent and a nutritional supplement. Recently, the potential usefulness of UDA as a neuroprotective drug has been suggested based on the ability of this agent to inhibit μ-calpain activity. In order to verify neuroprotective potential of UDA, we tested protective efficacy of this compound against cell damage evoked by pro-apoptotic factors (staurosporine and doxorubicin) and oxidative stress (hydrogen peroxide) in human neuroblastoma SH-SY5Y cells. We showed that UDA partially protected SH-SY5Y cells against the staurosporine- and doxorubicin-evoked cell death; however, this effect was not connected with its influence on caspase-3 activity. UDA decreased the St-induced changes in mitochondrial and cytosolic AIF level, whereas in Dox-model it affected only the cytosolic AIF content. Moreover, UDA (1-40 μM) decreased the hydrogen peroxide-induced cell damage which was connected with attenuation of hydrogen peroxide-mediated necrotic (PI staining, ADP/ATP ratio) and apoptotic (mitochondrial membrane potential, caspase-3 activation, AIF translocation) changes. Finally, we demonstrated that an inhibitor of PI3-K/Akt (LY294002) but not MAPK/ERK1/2 (U0126) pathway blocked the protection mediated by UDA in all tested models of SH-SY5Y cell injury. These in vitro data point to UDA as potentially effective neuroprotectant the utility of which should be further validated in animal studies. © 2015 Wiley Periodicals, Inc.

Latent Factors in Attention Emerge from 9 Years of Age among Elementary School Children

OpenAIRE

Tao, Ting; Wang, Ligang; Fan, Chunlei; Gao, Wenbin; Shi, Jiannong

2017-01-01

We explored the development of attention among elementary school children. Three hundred and sixty-five primary school children aged 7–12 years completed seven attention tests (alertness, focused attention, divided attention, attentional switching, sustained attention, spatial attention, and supervisory attention). A factor analysis indicated that there was no stable construct of attention among 7- to 8-year-old children. However, from 9 years on, children’s attention could be separated into ...

Quarterly review of 241-SY-101 mixer pump data: January - March 1999; FINAL

International Nuclear Information System (INIS)

CONNER, J.M.

1999-01-01

This report presents data obtained on 241-SY-101 pump performance. The period covered is January 1 through March 31, 1999. During the quarter: There were changes in pumping parameters. Both the pump volute pressure and amperage decreased during the quarter. It is not clear whether this was due to changes in waste properties (due to less solids or more entrained gas) or due to degradation of the pump. There was an indication of a 7.5-inch increase in the waste level at riser 1 A, and an average growth rate of 0.082 inches per day. There was an indication of a 5.7-inch increase in the waste level at riser 1C. This riser was flushed with water several times, which would lower the level of the crust at this location. Gases continued to be released at less than the pre-pump installation baseline rate, indicating a decrease in the gas generation rate, or an increase in gas retention, or both. The release rate was about 78 percent of the rate in the previous few quarters, and only 34 percent of the generation rate calculated prior to mixer pump installation in 1993. Key controls exist for waste temperature, gas concentration, pump parameters, and long-term waste behavior associated with the safe operation of the mixer pump that mitigates the buoyant displacement gas release event behavior of 241-SY-101. Table 1-1 compares the key controls and the current state of the waste as of March 3 1. 1999. The pump was run 28 times between January 1 and March 31, 1999. All of the pump runs were intended to be normal 25-minute, 1000-rpm excavation runs performed to mix the waste and release gas. Because of the pump oil often reached the high temperature alarm setpoint of 190 F, many of the runs were shortened (by as many as 8 minutes). This phenomenon was identified in November 1998, but got progressively worse over the quarter. The pump schedule was nominally three runs per week. However, core sampling activities interrupted the usual pump schedule several times during the quarter

A fluorescence assay for measuring acetylcholinesterase activity in rat blood and a human neuroblastoma cell line (SH-SY5Y).

Science.gov (United States)

Santillo, Michael F; Liu, Yitong

2015-01-01

Acetylcholinesterase (AChE) is an enzyme responsible for metabolism of the neurotransmitter acetylcholine, and inhibition of AChE can have therapeutic applications (e.g., drugs for Alzheimer's disease) or neurotoxic consequences (e.g., pesticides). A common absorbance-based AChE activity assay that uses 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) can have limited sensitivity and be prone to interference. Therefore, an alternative assay was developed, in which AChE activity was determined by measuring fluorescence of resorufin produced from coupled enzyme reactions involving acetylcholine and Amplex Red (10-acetyl-3,7-dihydroxyphenoxazine). The Amplex Red assay was used for two separate applications. First, AChE activity was measured in rat whole blood, which is a biomarker for exposure to AChE inhibitor pesticides. Activity was quantified from a 10(5)-fold dilution of whole blood, and there was a linear correlation between Amplex Red and DTNB assays. For the second application, Amplex Red assay was used to measure AChE inhibition potency in a human neuroblastoma cell line (SH-SY5Y), which is important for assessing pharmacological and toxicological potential of AChE inhibitors including drugs, phytochemicals, and pesticides. Five known reversible inhibitors were evaluated (IC50, 7-225 nM), along with irreversible inhibitors chlorpyrifos-oxon (ki=1.01 nM(-1)h(-1)) and paraoxon (ki=0.16 nM(-1)h(-1)). Lastly, in addition to inhibition, AChE reactivation was measured in SH-SY5Y cells incubated with pralidoxime chloride (2-PAM). The Amplex Red assay is a sensitive, specific, and reliable fluorescence method for measuring AChE activity in both rat whole blood and cultured SH-SY5Y cells. Published by Elsevier Inc.

Inhibition of WNT signaling reduces differentiation and induces sensitivity to doxorubicin in human malignant neuroblastoma SH-SY5Y cells.

Science.gov (United States)

Suebsoonthron, Junjira; Jaroonwitchawan, Thiranut; Yamabhai, Montarop; Noisa, Parinya

2017-06-01

Neuroblastoma is one of the most common cancers in infancy, arising from the neuroblasts during embryonic development. This cancer is difficult to treat and resistance to chemotherapy is often found; therefore, clinical trials of novel therapeutic approaches, such as targeted-cancer signaling, could be an alternative for a better treatment. WNT signaling plays significant roles in the survival, proliferation, and differentiation of human neuroblastoma. In this report, WNT signaling of a malignant human neuroblastoma cell line, SH-SY5Y cells, was inhibited by XAV939, a specific inhibitor of the Tankyrase enzyme. XAV939 treatment led to the reduction of β-catenin within the cells, confirming its inhibitory effect of WNT. The inhibition of WNT signaling by XAV939 did not affect cell morphology, survival, and proliferation; however, the differentiation and sensitivity to anticancer drugs of human neuroblastoma cells were altered. The treatment of XAV939 resulted in the downregulation of mature neuronal markers, including β-tubulin III, PHOX2A, and PHOX2B, whereas neural progenitor markers (PAX6, TFAP2α, and SLUG) were upregulated. In addition, the combination of XAV939 significantly enhanced the sensitivity of SH-SY5Y and IMR-32 cells to doxorubicin in both 2D and 3D culture systems. Microarray gene expression profiling suggested numbers of candidate target genes of WNT inhibition by XAV939, in particular, p21, p53, ubiquitin C, ZBED8, MDM2, CASP3, and FZD1, and this explained the enhanced sensitivity of SH-SY5Y cells to doxorubicin. Altogether, these results proposed that the altered differentiation of human malignant neuroblastoma cells by inhibiting WNT signaling sensitized the cells to anticancer drugs. This approach could thus serve as an effective treatment option for aggressive brain malignancy.

School Start Times for Middle School and High School Students - United States, 2011-12 School Year.

Science.gov (United States)

Wheaton, Anne G; Ferro, Gabrielle A; Croft, Janet B

2015-08-07

Adolescents who do not get enough sleep are more likely to be overweight; not engage in daily physical activity; suffer from depressive symptoms; engage in unhealthy risk behaviors such as drinking, smoking tobacco, and using illicit drugs; and perform poorly in school. However, insufficient sleep is common among high school students, with less than one third of U.S. high school students sleeping at least 8 hours on school nights. In a policy statement published in 2014, the American Academy of Pediatrics (AAP) urged middle and high schools to modify start times as a means to enable students to get adequate sleep and improve their health, safety, academic performance, and quality of life. AAP recommended that "middle and high schools should aim for a starting time of no earlier than 8:30 a.m.". To assess state-specific distributions of public middle and high school start times and establish a pre-recommendation baseline, CDC and the U.S. Department of Education analyzed data from the 2011-12 Schools and Staffing Survey (SASS). Among an estimated 39,700 public middle, high, and combined schools* in the United States, the average start time was 8:03 a.m. Overall, only 17.7% of these public schools started school at 8:30 a.m. or later. The percentage of schools with 8:30 a.m. or later start times varied greatly by state, ranging from 0% in Hawaii, Mississippi, and Wyoming to more than three quarters of schools in Alaska (76.8%) and North Dakota (78.5%). A school system start time policy of 8:30 a.m. or later provides teenage students the opportunity to achieve the 8.5-9.5 hours of sleep recommended by AAP and the 8-10 hours recommended by the National Sleep Foundation.

Neuronal-like differentiated SH-SY5Y cells adaptation to a mild and transient H2 O2 -induced oxidative stress.

Science.gov (United States)

Akki, Rachid; Siracusa, Rosalba; Morabito, Rossana; Remigante, Alessia; Campolo, Michela; Errami, Mohammed; La Spada, Giuseppina; Cuzzocrea, Salvatore; Marino, Angela

2018-03-01

Preconditioning (PC) is a cell adaptive response to oxidative stress and, with regard to neurons, can be considered as a neuroprotective strategy. The aim of the present study was to verify how neuronal-like differentiated SH-SY5Y cells adapt to a mild and transient H 2 O 2 -induced oxidative stress and, hence, whether may be considered as more sensitive cell model to study PC pathways. A first screening allowed to define H 2 O 2 concentrations for PC (10μM-50μM), applied before damage(100μM H 2 O 2 ). Cell viability measured 24 hours after 100μM H 2 O 2 -induced damage was ameliorated by 24-hour pre-exposure to low-concentration H 2 O 2 (10μM-30μM) with cell size as well restored. Markers for apoptosis (Bcl-2 and Bad), inflammation (iNOS), and redox system (MnSOD) were also determined, showing that, in cells pre-exposed to 10μM H 2 O 2 and then submitted to 100μM H 2 O 2 , Bcl-2 levels were higher, Bad and iNOS levels were lower than those observed in damaged cells, and MnSOD levels were unchanged. Such findings show that (1) neuronal-like differentiated SH-SY5Y cells are a suitable model to investigate PC response and more sensitive to the effect of a mild and transient H 2 O 2 -induced oxidative stress with respect to other neuronal cells; (2) 10μM H 2 O 2 -induced PC is mediated by apoptotic and inflammatory pathways, unlike antioxidant system; (3) such neuroprotective strategy and underlying signals proven in neuronal-like differentiated SH-SY5Y cells may contribute to understand in vivo PC mechanisms and to define a window for pharmacological intervention, namely, related to ischemic brain damage. Neuronal-like differentiated SH-SY5Y cells are a suitable model to investigate PC, an endogenous neuroprotective response to a mild and transient H 2 O 2 -induced oxidative stress, elicited by 24-hour exposure to very low H 2 O 2 concentrations and mediated by both apoptotic and inflammatory pathways. This model reflects in vivo PC mechanisms occurring

The influence of the school year on measles epidemics

DEFF Research Database (Denmark)

Andreasen, Viggo

The measles incidence record for Copenhagen 1880-1966 shows that the date of admission of new pupils has major impact on the structure of the epidemics, suggesting that measles transmission should be modelled in a way that accounts for the pulsed influx of new pupils. Assuming that the school year...

Entry-Year Administrator Induction: A State and Local School District Model.

Science.gov (United States)

Drury, William R.

1988-01-01

The Dayton (Ohio) City School District initiated a very successful pilot induction program for entry-year administrators in January 1987. Nine special workshops were planned to train both volunteer mentors and entry-year administrators in such areas as personal development, conflict management, problem identification and solution, time management,…

241-SY-101 strain concentration factor development via nonlinear analysis. Volume 1 of 1

International Nuclear Information System (INIS)

1997-01-01

The 241-SY-101 waste storage tank at the Hanford-Site has been known to accumulate and release significant quantities of hydrogen gas. An analysis was performed to assess the tank's structural integrity when subjected to postulated hydrogen deflagration loads. The analysis addressed many nonlinearities and appealed to a strain-based failure criteria. The model used to predict the global response of the tank was not refined enough to confidently predict local peak strains. Strain concentration factors were applied at structural discontinuities that were based on steel-lined reinforced-concrete containment studies. The discontinuities included large penetrations, small penetrations, springline geometries, stud/liner connections, and the 1/2 inch to 3/8 inch liner thickness transition. The only tank specific strain concentration factor applied in the evaluation was for the 1/2 inch to 3/8 inch liner thickness change in the dome. Review of the tank drawings reveals the possibility that a 4 inches Sch. 40 pipe penetrates the dome thickness transition region. It is not obvious how to combine the strain concentration factors for a small penetration with that of a thickness transition to arrive at a composite strain concentration factor. It is the goal of this effort to make an approximate determination of the relative significance of the 4 inch penetration and the 1/2 inch to 3/8 inch thickness transition in the 241-SY-101 dome geometry. This is accomplished by performing a parametric study with three general finite-element models. The first represents the thickness transition only, the second represents a 4 inch penetration only, and the third combines the thickness transition with a penetration model

Sasang constitutional types for the risk prediction of metabolic syndrome: a 14-year longitudinal prospective cohort study.

Science.gov (United States)

Lee, Sunghee; Lee, Seung Ku; Kim, Jong Yeol; Cho, Namhan; Shin, Chol

2017-09-02

To examine whether the use of Sasang constitutional (SC) types, such as Tae-yang (TY), Tae-eum (TE), So-yang (SY), and So-eum (SE) types, increases the accuracy of risk prediction for metabolic syndrome. From 2001 to 2014, 3529 individuals aged 40 to 69 years participated in a longitudinal prospective cohort. The Cox proportional hazard model was utilized to predict the risk of developing metabolic syndrome. During the 14 year follow-up, 1591 incident events of metabolic syndrome were observed. Individuals with TE type had higher body mass indexes and waist circumferences than individuals with SY and SE types. The risk of developing metabolic syndrome was the highest among individuals with the TE type, followed by the SY type and the SE type. When the prediction risk models for incident metabolic syndrome were compared, the area under the curve for the model using SC types was significantly increased to 0.8173. Significant predictors for incident metabolic syndrome were different according to the SC types. For individuals with the TE type, the significant predictors were age, sex, body mass index (BMI), education, smoking, drinking, fasting glucose level, high-density lipoprotein (HDL) cholesterol level, systolic and diastolic blood pressure, and triglyceride level. For Individuals with the SE type, the predictors were sex, smoking, fasting glucose, HDL cholesterol level, systolic and diastolic blood pressure, and triglyceride level, while the predictors in individuals with the SY type were age, sex, BMI, smoking, drinking, total cholesterol level, fasting glucose level, HDL cholesterol level, systolic and diastolic blood pressure, and triglyceride level. In this prospective cohort study among 3529 individuals, we observed that utilizing the SC types significantly increased the accuracy of the risk prediction for the development of metabolic syndrome.

[Is there an association between the reduced school years in grammar schools and headache and other health complaints in adolescent students?].

Science.gov (United States)

Milde-Busch, A; Blaschek, A; Borggräfe, I; von Kries, R; Straube, A; Heinen, F

2010-07-01

The reduction of school years in grammar schools from 9 to 8 years (G9 vs. G8) is supposed to exhibit increased impairments of health of the latter group of students. Aim of the present study was to investigate whether G8-students are exposed to more stress and report more headaches and other health complaints than G9-students. 1 260 formers of grammar schools in Munich (10 (th) vs. 11 (th) form). In a survey, the frequency of headache and other health complaints, experience of chronic stress and health-related quality of life were assessed with a questionnaire and compared between the two groups of different grammar-school durations (G8 vs. G9). 83.1% of all formers reported to suffer from headache at least once per month. Further frequently reported health complaints were back pain (47.7%), excessive need for sleep (45.6%) and pain in neck or shoulder (45.0%). 20.4% of the formers reported high exposure to stress. The greatest reductions in quality of life were found with respect to school-related and physical wellbeing. As the only significant differences, formers of G8 reported fewer daily leisure time and that available leisure time was not sufficient for recreation. The high prevalence of pain, health complaints and stress indicates high demands to all grammar scholars. High demands due to the reduction of school years in grammar school, however, are not reflected in increased health impairments in these formers, but rather in limited leisure time activities. (c) Georg Thieme Verlag KG Stuttgart-New York.

Lessons learned from 15 years of non-grades-based selection for medical school

NARCIS (Netherlands)

K.M. Stegers-Jager (Karen)

2018-01-01

textabstractContext: Thirty years ago, it was suggested in the Edinburgh Declaration that medical school applicants should be selected not only on academic, but also on non-academic, attributes. The main rationale behind extending medical school selection procedures with the evaluation of

Tackling Illiteracy in Year 7 of the Comprehensive School

Science.gov (United States)

Mays, Dennis

2012-01-01

Reading standards are regarded by many as the true yardstick by which to measure school effectiveness, and the relative standards achieved by different generations are often debated by adults. Some young people do not perceive reading to be of anything like such importance, however, using digital communication habitually and easily. Each year a…

prevalence of sleep disorders in khorramabad 7-12 year old elementary school children in school year 2006-2007

Directory of Open Access Journals (Sweden)

azam Mohsenzadeh

2009-11-01

Full Text Available The most important consequence of sleep disorders in children is cognitive dysfunction that leads to study, family and social disturbances. This study was carried out to evaluate the prevalence of sleep disorders in Khorramabad 7-12 year old elementary school children in school year 2006-2007. Materials and Methods: In this cross sectional study, 364 students were selected randomly in both sexes male and female with equal numbers. Data were collected using TUCASA questionnaire. Results: Results showed the revalence of sleep disorders as follows: mouth breathing 35/7%, sleep talking 24/7%, habitual snoring 20/3%, nightmare 19/8%, sleep teeth grinding 15/9%, secondary enuresis 8/2%, primary nocturnal enuresis 7/1%, sleep apnea 6/6%, sleep walking 6/6% and excessive daytime sleepiness 10%. Statistical tests showed that there is a significant relation between primary and secondary nocturnal enuresis and male sex, and both disorders were more in boys (p-value=0. 004. Between other disorders, and sex and age there was not significant relation. In this study between teeth grinding and snoring, sleep apnea and snoring, open mouth breathing and snoring, excessive daytime sleepiness and sleep apnea, sleep duration and time of sleep of parents, there was significant relation (p-value<0. 001. Conclusion: According to findings, mouth breathing was the most common sleep disorder in our subjects and had a significant relation with sleep snoring. So due to treating ability of nonmedical therapy in sleep disorders, it is recommended to increase parents information about necessity of medication and its effect on children cognition.

10 years with Planet Earth essence in the primary school children drawings

Science.gov (United States)

D'Addezio, Giuliana

2016-04-01

"10 years with Planet Earth" is the title of the 2016 INGV calendar for primary schools representing the review of a project conceived as support and complement of 15 years long INGV dissemination activities with schools. We realized 10 calendars together with and for primary schools, every year with a subject related to a World in constant evolution. Earthquakes, volcanic eruptions, tsunami waves, magnetic storms and other phenomena are manifestations of the complexity and dynamicity, which began more than four billion years ago and never halted. Since the Earth originated to the first presence of water, life and oxygen, the Cambrian explosion of species, the domain of dinosaurs, the great extinctions and glaciations, the surface of our planet experiences continents collisions, mountains and oceans formation and life forms emerging and disappearing. Every year we have launched a competition asking children to send drawings on themes chosen to stimulate learning about Earth Sciences and Planet Earth dynamics. We intended to raise awareness on issues as water resources availability, protection against natural disasters and control of environmental degradation. For each competition, we chose the most significant drawings to be included in the yearly calendar about the Earth. The authors of drawings were awarded by scientists, journalists, artists and science communicators and even by a minister. Besides the competitions, these drawings depict their own impressions and reflections, providing an opportunity to illustrate the children's point of view. From drawings and texts arise a great consideration and respect for the Planet, raising hopes that similar initiatives can contribute to increase the knowledge of the Earth and of the fragile human ecosystem in the hearts and minds of future active citizens. The project was made possible thanks to the teachers and to the wonderful students of more than 200 schools that sent about 10,000 drawings that have intrigued

Moving down the Track: Changing School Practices during the Second Year of "Diplomas Now"

Science.gov (United States)

Sepanik, Susan; Corrin, William; Roy, David; Gray, Aracelis; Fernandez, Felix; Briggs, Ashley; Wang, Kathleen K.

2015-01-01

Too many students in high-poverty, urban communities drop out of high school, and too few graduate prepared for college and careers. Three national organizations--Talent Development Secondary, City Year, and Communities In Schools--have formed "Diplomas Now" in an effort to transform urban secondary schools so fewer students drop out and…

Synthesis of reduced-size gold nanostars and internalization in SH-SY5Y cells

KAUST Repository

Dacarro, Giacomo

2017-07-01

The synthesis of large pentatwinned five-branched gold nanostars (GNS) has been modified so to obtain overall dimensions shrunk to 60% and a lower branches aspect ratio, leading to a dramatic blue shift of their two near-infrared (NIR) localized surface plasmon resonances (LSPR) absorptions but still maintaining one LSPR in the biotransparent NIR range. The interactions of polyethylene glycol (PEG) coated large and shrunk GNS with SH-SY5Y cells revealed that the large ones (DCI - diameter of the circumference in which GNS can be inscribed = 76 nm) are internalized more efficiently than the shrunk ones (DCI = 46 nm), correlating with a decreased cells surving fraction.

Synthesis of reduced-size gold nanostars and internalization in SH-SY5Y cells

KAUST Repository

Dacarro, Giacomo; Pallavicini, Piersandro; Bertani, Serena Maria; Chirico, Giuseppe; D'Alfonso, Laura; Falqui, Andrea; Marchesi, Nicoletta; Pascale, Alessia; Sironi, Laura; Taglietti, Angelo; Zuddas, Efisio

2017-01-01

The synthesis of large pentatwinned five-branched gold nanostars (GNS) has been modified so to obtain overall dimensions shrunk to 60% and a lower branches aspect ratio, leading to a dramatic blue shift of their two near-infrared (NIR) localized surface plasmon resonances (LSPR) absorptions but still maintaining one LSPR in the biotransparent NIR range. The interactions of polyethylene glycol (PEG) coated large and shrunk GNS with SH-SY5Y cells revealed that the large ones (DCI - diameter of the circumference in which GNS can be inscribed = 76 nm) are internalized more efficiently than the shrunk ones (DCI = 46 nm), correlating with a decreased cells surving fraction.

Radon testing in schools in New York State: a 20-year summary

International Nuclear Information System (INIS)

Kitto, Michael

2014-01-01

For nearly 20 years the Department of Health has conducted programs to assist in the measurement and reduction of indoor radon concentrations in 186 schools located primarily in Zone 1 areas of New York State. Although many schools had few or no rooms containing radon above 148 Bq/m 3 , some rooms had >740 Bq/m 3 and remediation techniques were utilized to reduce exposure. Short-term radon measurements in the schools showed little correlation to basement and first-floor radon results from single-family homes in the towns. - Highlights: • Relatively few schools in New York State have been tested for indoor radon. • We provide a summary of radon-testing results for measured schools. • The radon potential in schools is often less than in local houses. • Short-term measurement results exceeded their long-term counterparts in nearly every case

Effects of parent-child affective quality during high school years on subsequent substance use.

Directory of Open Access Journals (Sweden)

Ekaterina S. Ralston

2012-04-01

Full Text Available The literature indicates that the quality of affective relationships between youth and parents is associated with lower levels of a range of problem behaviors during childhood, early and late adolescence. While the protective effect of parental monitoring on substance use in the high school and post high school years has been demonstrated, there is a knowledge gap concerning effects of parent-child affective quality (PCAQ during the same periods. We tested a conceptual theoretical model to examine the effects of PCAQ on substance use following high school. The sample was from a RCT that assessed adolescents in rural Iowa from the seventh grade through two years after high school (N=456. We specified direct effects of PCAQ in 12th grade on drunkenness, smoking and illicit drug use during the two years immediately following high school graduation. We also specified the effects of early substance use initiation (alcohol, tobacco and marijuana use reported at baseline on later use. The direct effect of PCAQ in 12th grade on substance use was significant for all substances during at least one of the two years past graduation (ypg. Results were: drunkenness 1 ypg, β=-.126, p<.05; smoking 1 ypg, β=-.119, p<.05; 2 ypg, β=-.146, p<.05; illicit drug use 2 ypg, β=-.165, p<.05. Some significant indirect effects of PCAQ at baseline, via PCAQ at 12th grade, were found. Results also indicated significant direct effects of early initiation on two of the three substances, albeit with a different pattern of effects over time for each substance by years post high school. Importantly, while early initiation remains the strongest predictor of long-term tobacco and illicit drug use, results show how PCAQ might reduce its harmful effects.

Smoking Prevention for Students: Findings From a Three-Year Program of Integrated Harm Minimization School Drug Education.

Science.gov (United States)

Midford, Richard; Cahill, Helen; Lester, Leanne; Foxcroft, David R; Ramsden, Robyn; Venning, Lynne

2016-01-01

This study investigated the impact of the Drug Education in Victorian Schools (DEVS) program on tobacco smoking. The program taught about licit and illicit drugs in an integrated manner over 2 years, with follow up in the third year. It focused on minimizing harm, rather than achieving abstinence, and employed participatory, critical-thinking and skill-based teaching methods. A cluster-randomized, controlled trial of the program was conducted with a student cohort during years 8 (13 years), 9 (14 years), and 10 (15 years). Twenty-one schools were randomly allocated to the DEVS program (14 schools, n = 1163), or their usual drug education program (7 schools, n = 589). One intervention school withdrew in year two. There was a greater increase in the intervention students' knowledge about drugs, including tobacco, in all 3 years. Intervention students talked more with their parents about smoking at the end of the 3-year program. They recalled receiving more education on smoking in all 3 years. Their consumption of cigarettes had not increased to the same extent as controls at the end of the program. Their change in smoking harms, relative to controls, was positive in all 3 years. There was no difference between groups in the proportionate increase of smokers, or in attitudes towards smoking, at any time. These findings indicate that a school program that teaches about all drugs in an integrated fashion, and focuses on minimizing harm, does not increase initiation into smoking, while providing strategies for reducing consumption and harm to those who choose to smoke.

Kick off of the 2017-2018 school year at the EVE and School of the CERN Staff Association

CERN Multimedia

Staff Association

2017-01-01

The Children’s Day-Care Centre (“Espace de Vie Enfantine” - EVE) and School of the CERN Staff Association opened its doors once again to welcome the children, along with the teaching and administrative staff of the structure. The start of the school year was carried out gradually and in small groups to allow quality interaction between children, professionals and parents. At the EVE (Nursery and Kindergarten) and School, the children have the opportunity to thrive in a privileged environment, rich in cultural diversity, since the families (parents and children) come from many different nationalities. The teaching staff do their utmost to ensure that the children can become more autonomous and develop their social skills, all the while taking care of their well-being. This year, several new features are being introduced, for instance, first steps towards English language awareness. Indeed, the children will get to discover the English language in creative classes together with tr...

Oxidative stress response in SH-SY5Y cells exposed to short-term 1800 MHz radiofrequency radiation.

Science.gov (United States)

Marjanovic Cermak, Ana Marija; Pavicic, Ivan; Trosic, Ivancica

2018-01-28

The exact mechanism that could explain the effects of radiofrequency (RF) radiation exposure at non-thermal level is still unknown. Increasing evidence suggests a possible involvement of reactive oxygen species (ROS) and development of oxidative stress. To test the proposed hypothesis, human neuroblastoma cells (SH-SY5Y) were exposed to 1800 MHz short-term RF exposure for 10, 30 and 60 minutes. Electric field strength within Gigahertz Transverse Electromagnetic cell (GTEM) was 30 V m -1 and specific absorption rate (SAR) was calculated to be 1.6 W kg -1 . Cellular viability was measured by MTT assay and level of ROS was determined by fluorescent probe 2',7'-dichlorofluorescin diacetate. Concentrations of malondialdehyde and protein carbonyls were used to assess lipid and protein oxidative damage and antioxidant activity was evaluated by measuring concentrations of total glutathione (GSH). After radiation exposure, viability of irradiated cells remained within normal physiological values. Significantly higher ROS level was observed for every radiation exposure time. After 60 min of exposure, the applied radiation caused significant lipid and protein damage. The highest GSH concentration was detected after 10 minute-exposure. The results of our study showed enhanced susceptibility of SH-SY5Y cells for development of oxidative stress even after short-term RF exposure.

Le paysage atlantique dans Lettres à ma fille de Jean Désy. Entre projection et plongée

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Myriam Marcil-Bergeron

2015-11-01

Full Text Available L’écrivain-voyageur Jean Désy raconte dans Lettres à ma fille (1997 sa première navigation hauturière, qui consiste en une traversée de l’océan Atlantique à la voile. Deux pôles orientent la représentation du grand large dans ce récit, soit la projection du voyageur vers le dehors et la plongée dans ses souvenirs des paysages nordiques et sylvestres. Par une approche géopoétique, cet article vise à examiner les effets du rythme et de la polysensorialité ainsi que la manière dont l’expérience du Nord et de la forêt participe à la configuration du paysage atlantique. The traveller and writer Jean Désy narrates in Lettres à ma fille (1997 his first ocean navigation, which consists in a sailing across the Atlantic. In this story, two poles orient the representation of the open sea, namely the traveler’s outward projection and his diving into memories from nordic and forest landscapes. Through a geopoetic approach, this article aims to examine the effects of rhythm and polysensoriality, and also how the experience of the North and the forest participates in the configuration of the atlantic landscape.

Ursodeoxycholic acid suppresses mitochondria-dependent programmed cell death induced by sodium nitroprusside in SH-SY5Y cells

International Nuclear Information System (INIS)

Chun, Hong Sung; Low, Walter C.

2012-01-01

Although ursodeoxycholic acid (UDCA) and its highly water-soluble formula (Yoo's solution; YS) have been shown to prevent neuronal damage, the effects of UDCA or YS against Parkinson's disease (PD)-related dopaminergic cell death has not been studied. This study investigated the protective effects of UDCA and YS on sodium nitroprusside (SNP)-induced cytotoxicity in human dopaminergic SH-SY5Y cells. Both UDCA (50–200 μM) and YS (100–200 μM) dose-dependently prevented SNP (1 mM)-induced cell death. Results showed that both UDCA and YS effectively attenuated the production of total reactive oxygen species (ROS), peroxynitrite (ONOO − ) and nitric oxide (NO), and markedly inhibited the mitochondrial membrane potential (MMP) loss and intracellular reduced glutathione (GSH) depletion. SNP-induced programmed cell death events, such as nuclear fragmentation, caspase-3/7 and -9 activation, Bcl-2/Bax ratio decrease, and cytochrome c release, were significantly attenuated by both UDCA and YS. Furthermore, selective inhibitor of phosphatidylinositiol-3-kinase (PI3K), LY294002, and Akt/PKB inhibitor, triciribine, reversed the preventive effects of UDCA on the SNP-induced cytotoxicity and Bax translocation. These results suggest that UDCA can protect SH-SY5Y cells under programmed cell death process by regulating PI3K-Akt/PKB pathways.

Allergy sensitization and asthma among 13-14 year old school ...

African Journals Online (AJOL)

EB

Allergy sensitization and asthma among 13-14 year old school children in Nigeria. Oluwole O1 ... Identified asthma cases were matched to controls. Allergy skin tests ..... lower risk of asthma than those with low birth order because exposure to ...

Portland Public Schools Project Chrysalis: Year 2 Evaluation Report.

Science.gov (United States)

Mitchell, Stephanie J.; Gabriel, Roy M.; Hahn, Karen J.; Laws, Katherine E.

In 1994, the Chrysalis Project in Portland Public Schools received funding to prevent or delay the onset of substance abuse among a special target population: high-risk, female adolescents with a history of childhood abuse. Findings from the evaluation of the project's second year of providing assistance to these students are reported here. During…

School results and access test results as indicators of first-year performance at university

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A Bothma

2004-06-01

Full Text Available The goals set by the National Plan for Higher Education, the fact that many schools are still severely disadvantaged as well as far-reaching changes in the school system demand that South African universities urgently reconsider their admission procedures. Redesigning admission procedures calls for a thorough understanding of the interrelationships between school marks, results in existing access tests and first-year university performance. These interrelationships were statistically investigated in the case of the 1999, 2000 and 2001 intake groups, who were compelled to write access tests before being admitted to Stellenbosch University. The results of this investigation confirm an alarming degree of unpreparedness among many prospective students regarding what is expected of them at university. This is aggravated by school marks creating a totally unrealistic expectation of performance in the first year at university. It is emphasised that schools and authorities dealing with admission of prospective students at universities should be cognisant of the findings reported here. Furthermore, the statistical analyses demonstrate several novel techniques for investigating the interrelationship between school marks, access test results and university performance.

Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells

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João Barbosa Martins

2013-01-01

Full Text Available Paraquat (PQ, a cationic nonselective bipyridyl herbicide, has been used as neurotoxicant to modulate Parkinson’s disease in laboratory settings. Other compounds like rotenone (ROT, a pesticide, and 1-methyl-4-phenylpyridinium ion (MPP+ have been widely used as neurotoxicants. We compared the toxicity of these three neurotoxicants using differentiated dopaminergic SH-SY5Y human cells, aiming to elucidate their differential effects. PQ-induced neurotoxicity was shown to be concentration and time dependent, being mitochondrial dysfunction followed by neuronal death. On the other hand, cells exposure to MPP+ induced mitochondrial dysfunction, but not cellular lyses. Meanwhile, ROT promoted both mitochondrial dysfunction and neuronal death, revealing a biphasic pattern. To further elucidate PQ neurotoxic mechanism, several protective agents were used. SH-SY5Y cells pretreatment with tiron (TIR and 2-hydroxybenzoic acid sodium salt (NaSAL, both antioxidants, and Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME, a nitric oxide synthase inhibitor, partially protected against PQ-induced cell injury. Additionally, 1-(2-[bis(4-fluorophenylmethoxy]ethyl-4-(3-phenyl-propylpiperazine (GBR 12909, a dopamine transporter inhibitor, and cycloheximide (CHX, a protein synthesis inhibitor, also partially protected against PQ-induced cell injury. In conclusion, we demonstrated that PQ, MPP+, and ROT exerted differential toxic effects on dopaminergic cells. PQ neurotoxicity occurred through exacerbated oxidative stress, with involvement of uptake through the dopamine transporter and protein synthesis.

Employment and First Year Experience of Beginner Primary School Teachers at Private Educational Institutions

OpenAIRE

Turgay ÖNTAŞ

2016-01-01

In this study to demonstrate primary school teachers' employment process and their first year teaching in private educational organizations is aimed. The major employer of primary school teachers is public schools that are funded by state. There will be a gap between grade inflation and employment rate when all of the teacher candidates claim to be appointed to public schools. Hence private educational organizations provide alternative career opportunities for teacher candidates. Qualitative ...

Specific pesticide-dependent increases in α-synuclein levels in human neuroblastoma (SH-SY5Y) and melanoma (SK-MEL-2) cell lines.

Science.gov (United States)

Chorfa, Areski; Bétemps, Dominique; Morignat, Eric; Lazizzera, Corinne; Hogeveen, Kevin; Andrieu, Thibault; Baron, Thierry

2013-06-01

Epidemiological studies indicate a role of genetic and environmental factors in Parkinson's disease involving alterations of the neuronal α-synuclein (α-syn) protein. In particular, a relationship between Parkinson's disease and occupational exposure to pesticides has been repeatedly suggested. Our objective was to precisely assess changes in α-syn levels in human neuroblastoma (SH-SY5Y) and melanoma (SK-MEL-2) cell lines following acute exposure to pesticides (rotenone, paraquat, maneb, and glyphosate) using Western blot and flow cytometry. These human cell lines express α-syn endogenously, and overexpression of α-syn (wild type or mutated A53T) can be obtained following recombinant adenoviral transduction. We found that endogenous α-syn levels in the SH-SY5Y neuroblastoma cell line were markedly increased by paraquat, and to a lesser extent by rotenone and maneb, but not by glyphosate. Rotenone also clearly increased endogenous α-syn levels in the SK-MEL-2 melanoma cell line. In the SH-SY5Y cell line, similar differences were observed in the α-syn adenovirus-transduced cells, with a higher increase of the A53T mutated protein. Paraquat markedly increased α-syn in the SK-MEL-2 adenovirus-transduced cell line, similarly for the wild-type or A53T proteins. The observed differences in the propensities of pesticides to increase α-syn levels are in agreement with numerous reports that indicate a potential role of exposure to certain pesticides in the development of Parkinson's disease. Our data support the hypothesis that pesticides can trigger some molecular events involved in this disease and also in malignant melanoma that consistently shows a significant but still unexplained association with Parkinson's disease.

Evaluation of the neurotoxic/neuroprotective role of organoselenides using differentiated human neuroblastoma SH-SY5Y cell line challenged with 6-hydroxydopamine.

Science.gov (United States)

Lopes, Fernanda Martins; Londero, Giovana Ferreira; de Medeiros, Liana Marengo; da Motta, Leonardo Lisbôa; Behr, Guilherme Antônio; de Oliveira, Valeska Aguiar; Ibrahim, Mohammad; Moreira, José Cláudio Fonseca; Porciúncula, Lisiane de Oliveira; da Rocha, João Batista Teixeira; Klamt, Fábio

2012-08-01

It is well established that oxidative stress plays a major role in several neurodegenerative conditions, like Parkinson disease (PD). Hence, there is an enormous effort for the development of new antioxidants compounds with therapeutic potential for the management of PD, such as synthetic organoselenides molecules. In this study, we selected between nine different synthetic organoselenides the most eligible ones for further neuroprotection assays, using the differentiated human neuroblastoma SH-SY5Y cell line as in vitro model. Neuronal differentiation of exponentially growing human neuroblastoma SH-SY5Y cells was triggered by cultivating cells with DMEM/F12 medium with 1% of fetal bovine serum (FBS) with the combination of 10 μM retinoic acid for 7 days. Differentiated cells were further incubated with different concentrations of nine organoselenides (0.1, 0.3, 3, 10, and 30 μM) for 24 h and cell viability, neurites densities and the immunocontent of neuronal markers were evaluated. Peroxyl radical scavenging potential of each compound was determined with TRAP assay. Three organoselenides tested presented low cytotoxicity and high antioxidant properties. Pre-treatment of cells with those compounds for 24 h lead to a significantly neuroprotection against 6-hydroxydopamine (6-OHDA) toxicity, which were directly related to their antioxidant properties. Neuroprotective activity of all three organoselenides was compared to diphenyl diselenide (PhSe)₂, the simplest of the diaryl diselenides tested. Our results demonstrate that differentiated human SH-SY5Y cells are suitable cellular model to evaluate neuroprotective/neurotoxic role of compounds, and support further evaluation of selected organoselenium molecules as potential pharmacological and therapeutic drugs in the treatment of PD.

An Extract from Shrimp Processing By-Products Protects SH-SY5Y Cells from Neurotoxicity Induced by Aβ25–35

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Yongping Zhang

2017-03-01

Full Text Available Increased evidence suggests that marine unsaturated fatty acids (FAs can protect neurons from amyloid-β (Aβ-induced neurodegeneration. Nuclear magnetic resonance (NMR, high performance liquid chromatography (HPLC and gas chromatography (GC assays showed that the acetone extract 4-2A obtained from shrimp Pandalus borealis industry processing wastes contained 67.19% monounsaturated FAs and 16.84% polyunsaturated FAs. The present study evaluated the anti-oxidative and anti-inflammatory effects of 4-2A in Aβ25–35-insulted differentiated SH-SY5Y cells. Cell viability and cytotoxicity were measured by using 3-(4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT and lactate dehydrogenase (LDH assays. Quantitative PCR and Western blotting were used to study the expression of neurotrophins, pro-inflammatory cytokines and apoptosis-related genes. Administration of 20 μM Aβ25–35 significantly reduced SH-SY5Y cell viability, the expression of nerve growth factor (NGF and its tyrosine kinase TrkA receptor, as well as the level of glutathione, while increased reactive oxygen species (ROS, nitric oxide, tumor necrosis factor (TNF-α, brain derived neurotrophic factor (BDNF and its TrkB receptor. Aβ25–35 also increased the Bax/Bcl-2 ratio and Caspase-3 expression. Treatment with 4-2A significantly attenuated the Aβ25–35-induced changes in cell viability, ROS, GSH, NGF, TrkA, TNF-α, the Bax/Bcl-2 ratio and Caspase-3, except for nitric oxide, BDNF and TrKB. In conclusion, 4-2A effectively protected SH-SY5Y cells against Aβ-induced neuronal apoptosis/death by suppressing inflammation and oxidative stress and up-regulating NGF and TrKA expression.

Unusual negative magnetoresistance in Bi2Se3-ySy topological insulator under perpendicular magnetic field

Science.gov (United States)

Singh, Rahul; Gangwar, Vinod K.; Daga, D. D.; Singh, Abhishek; Ghosh, A. K.; Kumar, Manoranjan; Lakhani, A.; Singh, Rajeev; Chatterjee, Sandip

2018-03-01

The magneto-transport properties of Bi2Se3-ySy were investigated. Magnetoresistance (MR) decreases with an increase in the S content, and finally, for 7% (i.e., y = 0.21) S doping, the magnetoresistance becomes negative. This negative MR is unusual as it is observed when a magnetic field is applied in the perpendicular direction to the plane of the sample. The magneto-transport behavior shows the Shubnikov-de Haas (SdH) oscillation, indicating the coexistence of surface and bulk states. The negative MR has been attributed to the non-trivial bulk conduction.

SY-101 Rapid Transfer Project Low Temperature Operations Review and Recommendations to Support Lower Temperature Limits

International Nuclear Information System (INIS)

HICKMAN, G.L.

2000-01-01

The lower temperature limit for the 241 SY-101 RAPID transfer project is currently set at 20 F Based on the analysis and recommendations in this document this limit can be lowered to 0 F. Analysis of all structures systems and components (SSCs) indicate that a reduction in operating temperature may be achieved with minor modifications to field-installed equipment. Following implementation of these changes it is recommended that the system requirements be amended to specify a temperature range for transfer or back dilute evolutions of 0 F to 100 F

Philadelphia's Renaissance Schools Initiative after Four Years

Science.gov (United States)

Stratos, Kati; Wolford, Tonya; Reitano, Adrienne

2015-01-01

In 2010-2011, the School District of Philadelphia (the District) launched its Renaissance Schools Initiative, a program designed to dramatically improve student achievement in the District's lowest performing schools. Some schools became Promise Academies, based on the federal turnaround model, and remained District-operated neighborhood schools.…

Shaping Attitude toward Christianity among Year Seven Pupils: The Influence of Sex, Church, Home and Primary School

Science.gov (United States)

Swindells, Tracy; Francis, Leslie J.; Robbins, Mandy

2010-01-01

This study set out to examine the differences in attitude toward Christianity among year seven secondary school pupils who had undertaken their primary education either in a Church of England voluntary aided school or a non-denominational state-maintained school. Data were provided by 492 year-seven pupils attending three Church of England…

Developmental Links Between Children's Working Memory and their Social Relations with Teachers and Peers in the Early School Years.

Science.gov (United States)

de Wilde, Amber; Koot, Hans M; van Lier, Pol A C

2016-01-01

This study assessed the developmental links between children's working memory development and their relations with teachers and peers across 2 years of kindergarten and early elementary school. Kindergarten and first grade children, N = 1109, 50% boys, were followed across 2 school-years. Children were assessed across 3 waves, in the fall and spring of the first school-year (within school-year), and finally in the spring of the second school-year. Working memory was assessed using a visuo-spatial working memory task. The developmental links between working memory and child-reported teacher-child relationship quality (warmth and conflict) and peer-nominated likeability and friendedness were assessed using autoregressive cross-lagged models. Lower working memory scores were related to increases in teacher-child conflict and decreases in teacher-child warmth one school-year later, in addition to decreases in likeability by peers within the same school-year. Conversely, teacher-child conflict was negatively associated with the development of working memory across the studied period. Path estimates between working memory and social relational factors were similar for boys and girls. Findings show developmental links between working memory and social-relational factors and vice versa. These results suggest that children's working memory development can be fostered through pro-social relations with teachers in early elementary school children.

An Annotated Bibliography of Hypobaric Decompression Sickness Research Conducted at the Crew Technology Division, USAF School of Aerospace Medicine, Brooks AFB, Texas from 1983 to 1988

Science.gov (United States)

1990-06-01

AN ANNOTATED BIBLIOGRAPHY OF HYPOBARIC DECOMPRESSION SICKNESS RESEARCH CONDUCTED AT THE CREW TECHNOLOGY DIVISION, USAF SCHOOL OF AEROSPACE MEDICINE...190 man-flights to four selected altitudes (30000, 27500, 25000, and 22500 ft pressure equivalent) in a hypobaric chamber. The subjects’ ages ranged...conditions and two of these developed delayed sy~rtcms. Three of these five subjects underwent hyperbaric oxygen treatment. Conclusion. Female subjects

Does Year Round Schooling Affect the Outcome and Growth of California's API Scores?

Science.gov (United States)

Wu, Amery D.; Stone, Jake E.

2010-01-01

This paper examined whether year round schooling (YRS) in California had an effect upon the outcome and growth of schools' Academic Performance Index (API) scores. While many previous studies had examined the connection between YRS and academic achievement, most had lacked the statistical rigour required to provide reliable interpretations. As a…

Are Charter Schools Getting More Money into the Classroom? A Micro-Financial Analysis of First Year Charter Schools in Massachusetts.

Science.gov (United States)

Herdman, Paul; Millot, Marc Dean

This report examines the first-year finances of Massachusetts' first 15 charter schools. Analysis of the financial statements of these schools offers insights into how their boards of trustees allocated funds as they tried to meet academic goals and survive as new organizations. Revenue and expenditure patterns were compared with national district…

The Observation Of Defects Of School Buildings Over 100 Years Old In Perak

Directory of Open Access Journals (Sweden)

Alauddin Kartina

2016-01-01

Full Text Available Malaysia is blessed with a rich legacy of heritage buildings with unique architectural and historical values. The heritage buildings become a symbol of the national identity of our country. Therefore, heritage buildings, as important monuments should be conserved well to ensure the extension of the building’s life span and to make sure continuity functions of the building for future generations. The aim of this study is to analyze the types of defects attached in school buildings over 100 years located in Perak. The data were collected in four different schools aged over 100 years in Perak. The finding of the study highlighted the types of defects which were categorized based on building elements, including external wall, roof, door, ceiling, staircase, column, internal wall, floor and windows. Finding showed that the type of defects occurred in school buildings over 100 years in Perak is the same as the other heritage buildings. This finding can be used by all parties to take serious actions in preventing defects from occurring in buildings over 100 years. This would ensure that buildings’ functional life span can be extended for future use.

The relationship between first-year achievement and the pedagogical-didactical fit between secondary school and university

NARCIS (Netherlands)

Torenbeek, Marjolein; Jansen, Ellen; Hofman, Adriaan

2011-01-01

Central in this study is the degree to which the pedagogical-didactical approach in undergraduate programmes aligns with the pedagogical-didactical approach in secondary schools, and how this is related to first-year achievement. Approaches to teaching at secondary schools and in first-year

Promotion of SH-SY5Y Cell Growth by Gold Nanoparticles Modified with 6-Mercaptopurine and a Neuron-Penetrating Peptide

Science.gov (United States)

Xiao, Yaruo; Zhang, Enqi; Fu, Ailing

2017-12-01

Much effort has been devoted to the discovery of effective biomaterials for nerve regeneration. Here, we reported a novel application of gold nanoparticles (AuNPs) modified with 6-mercaptopurine (6MP) and a neuron-penetrating peptide (RDP) as a neurophic agent to promote proliferation and neurite growth of human neuroblastoma (SH-SY5Y) cells. When the cells were treated with 6MP-AuNPs-RDP conjugates, they showed higher metabolic activity than the control. Moreover, SH-SY5Y cells were transplanted onto the surface coated with 6MP-AuNPs-RDP to examine the effect of neurite development. It can be concluded that 6MP-AuNPs-RDP attached to the cell surface and then internalized into cells, leading to a significant increase of neurite growth. Even though 6MP-AuNPs-RDP-treated cells were recovered from frozen storage, the cells still maintained constant growth, indicating that the cells have excellent tolerance to 6MP-AuNPs-RDP. The results suggested that the 6MP-AuNPs-RDP had promising potential to be developed as a neurophic nanomaterial for neuronal growth.

High School Physics, Two-Year Colleges, and Physics Majors

Science.gov (United States)

White, Susan C.

2013-01-01

We have just completed the data collection for our 2012-13 Nationwide Survey of High School Physics and expect to have results to report in the spring. In the interim, we will take a look at physics in two-year colleges (TYCs). In 2007, we surveyed undergraduate seniors in degree-granting physics departments, and we asked these students if they…

SNJ-1945, a calpain inhibitor, protects SH-SY5Y cells against MPP+ and rotenone

Science.gov (United States)

Knaryan, Varduhi H.; Samantaray, Supriti; Sookyoung, Park; Azuma, Mitsuyoshi; Inoue, Jun; Banik, Naren L.

2014-01-01

Complex pathophysiology of Parkinson’s disease (PD) involves multiple CNS cell types. Degeneration in spinal cord neurons alongside brain has been shown to be involved in PD and evidenced in experimental parkinsonism. However, the mechanisms of these degenerative pathways are not well understood. In order to unravel these mechanisms SH-SY5Y neuroblastoma cells were differentiated into dopaminergic and cholinergic phenotypes respectively and used as cell culture model following exposure to two parkinsonian neurotoxicants MPP+ and rotenone. SNJ-1945, a cell-permeable calpain inhibitor was tested for its neuroprotective efficacy. MPP+ and rotenone dose-dependently elevated the levels of intracellular free Ca2+ and induced a concomitant rise in the levels of active calpain. SNJ-1945 pre-treatment significantly protected cell viability and preserved cellular morphology following MPP+ and rotenone exposure. The neurotoxicants elevated the levels of reactive oxygen species (ROS) more profoundly in SH-SY5Y cells differentiated into dopaminergic phenotype, and this effect could be attenuated with SNJ-1945 pre-treatment. In contrast, significant levels of inflammatory mediators (cyclooxygenase-2, Cox-2 and cleaved p10 fragment of caspase-1) were upregulated in the cholinergic phenotype, which could be dose-dependently attenuated by the calpain inhibitor. Overall, SNJ-1945 was efficacious against MPP+ or rotenone-induced ROS generation, inflammatory mediators, and proteolysis. A post-treatment regimen of SNJ-1945 was also examined in cells and partial protection was attained with calpain inhibitor administration 1–3 h after exposure to MPP+ or rotenone. Taken together these results indicate that calpain inhibition is a valid target for protection against parkinsonian neurotoxicants, and SNJ-1945 is an efficacious calpain inhibitor in this context. PMID:24341912

New Mexico School District Profile: 1982-83 School Year.

Science.gov (United States)

Cavatta, Jerry C., Comp.; Borgrink, Henry, Comp.

The purpose of this report is to provide a broad cross section of data on the operation and performance of New Mexico public schools. Data on school district characteristics (40-Day Average Daily Membership; and percent in kindergarten, bilingual, Chapter 1, and special education programs), teacher characteristics (pupil-teacher ratio, salaries,…

Math Anxiety and Math Ability in Early Primary School Years

Science.gov (United States)

Krinzinger, Helga; Kaufmann, Liane; Willmes, Klaus

2009-01-01

Mathematical learning disabilities (MLDs) are often associated with math anxiety, yet until now, very little is known about the causal relations between calculation ability and math anxiety during early primary school years. The main aim of this study was to longitudinally investigate the relationship between calculation ability, self-reported…

Do healthy school meals affect illness, allergies and school attendance in 8- to 11-year-old children? A cluster-randomised controlled study.

Science.gov (United States)

Laursen, R P; Lauritzen, L; Ritz, C; Dyssegaard, C B; Astrup, A; Michaelsen, K F; Damsgaard, C T

2015-05-01

A nutritionally adequate diet in childhood is important for health and resistance of allergies and infections. This study explored the effects of school meals rich in fish, vegetables and fibre on school attendance, asthma, allergies and illness in 797 Danish 8- to 11-year-old children. No comparable studies conducted in high-income settings have been identified. The OPUS School Meal Study was a cluster-randomised cross-over trial. Children from third and fourth grades at nine Danish schools received school meals or usual packed lunch (control) for two 3-month periods. Occurrence and duration of illnesses, asthma and allergies during the last 14 days were recorded by parental questionnaires at baseline and after each 3-month period. Self-reported well-being was assessed by visual analogue scales. The school meals did not affect school attendance, parent-reported occurrence or duration of asthma and allergies or self-reported well-being. The most common symptoms of illness were stomach pain (24%), headache (28%) and cold (24%). A slightly higher number of children experienced headaches in the school meal (27%) compared with the control period (22%) (P=0.02). However, subgroup analyses showed that this effect was only seen in children eating school meals in the classroom (P=0.007), and not in common dining areas (P=0.2). No effect was found on other symptoms of illness. Provision of nutritionally balanced school meals did not affect school attendance, asthma, allergies, illness or well-being in 8- to 11-year-old children. The slight increase in occurrence of headaches seems to be related to the physical eating environment.

Graphene Oxide–Silver Nanoparticles Nanocomposite Stimulates Differentiation in Human Neuroblastoma Cancer Cells (SH-SY5Y

Directory of Open Access Journals (Sweden)

Sangiliyandi Gurunathan

2017-11-01

Full Text Available Recently, graphene and graphene related nanocomposite receive much attention due to high surface-to-volume ratio, and unique physiochemical and biological properties. The combination of metallic nanoparticles with graphene-based materials offers a promising method to fabricate novel graphene–silver hybrid nanomaterials with unique functions in biomedical nanotechnology, and nanomedicine. Therefore, this study was designed to prepare graphene oxide (GO silver nanoparticles (AgNPs nanocomposite (GO-AgNPs containing two different nanomaterials in single platform with distinctive properties using luciferin as reducing agents. In addition, we investigated the effect of GO-AgNPs on differentiation in SH-SY5Y cells. The synthesized GO-AgNPs were characterized by ultraviolet-visible absorption spectroscopy (UV-vis, X-ray diffraction (XRD, scanning electron microscopy (SEM, transmission electron microscopy (TEM and Raman spectroscopy. The differentiation was confirmed by series of cellular and biochemical assays. The AgNPs were distributed uniformly on the surface of graphene oxide with an average size of 25 nm. As prepared GO-AgNPOs induces differentiation by increasing the expression of neuronal differentiation markers and decreasing the expression of stem cell markers. The results indicated that the redox biology involved the expression of various signaling molecules, which play an important role in differentiation. This study suggests that GO-AgNP nanocomposite could stimulate differentiation of SH-SY5Y cells. Furthermore, understanding the mechanisms of differentiation of neuroblastoma cells could provide new strategies for cancer and stem cell therapies. Therefore, these studies suggest that GO-AgNPs could target specific chemotherapy-resistant cells within a tumor.

Vaccination coverage among children in kindergarten - United States, 2013-14 school year.

Science.gov (United States)

Seither, Ranee; Masalovich, Svetlana; Knighton, Cynthia L; Mellerson, Jenelle; Singleton, James A; Greby, Stacie M

2014-10-17

State and local vaccination requirements for school entry are implemented to maintain high vaccination coverage and protect schoolchildren from vaccine-preventable diseases. Each year, to assess state and national vaccination coverage and exemption levels among kindergartners, CDC analyzes school vaccination data collected by federally funded state, local, and territorial immunization programs. This report describes vaccination coverage in 49 states and the District of Columbia (DC) and vaccination exemption rates in 46 states and DC for children enrolled in kindergarten during the 2013-14 school year. Median vaccination coverage was 94.7% for 2 doses of measles, mumps, and rubella (MMR) vaccine; 95.0% for varying local requirements for diphtheria, tetanus toxoid, and acellular pertussis (DTaP) vaccine; and 93.3% for 2 doses of varicella vaccine among those states with a 2-dose requirement. The median total exemption rate was 1.8%. High exemption levels and suboptimal vaccination coverage leave children vulnerable to vaccine-preventable diseases. Although vaccination coverage among kindergartners for the majority of reporting states was at or near the 95% national Healthy People 2020 targets for 4 doses of DTaP, 2 doses of MMR, and 2 doses of varicella vaccine, low vaccination coverage and high exemption levels can cluster within communities. Immunization programs might have access to school vaccination coverage and exemption rates at a local level for counties, school districts, or schools that can identify areas where children are more vulnerable to vaccine-preventable diseases. Health promotion efforts in these local areas can be used to help parents understand the risks for vaccine-preventable diseases and the protection that vaccinations provide to their children.

Children, childhood and schooling: adjustment in the transition from kindergarten to primary school nine years old

Directory of Open Access Journals (Sweden)

Marciel Barcelos

2015-09-01

Full Text Available The following article aims to understand the concepts of children, childhood and education of practitioners of everyday (Teachers graduated in physical education, conductor teacher graduated in Pedagogy and coordinator graduated in Physical Education EMEF "Espírito Santo". Therefore, ethnographic study case became attributed and used as narrative sources produced through record fields, interview and discussion groups. The results show the creation of strategies to incorporate in the children the school cultures. That path is produced by the author's experiences in producing moments that articulate the cultural practices of children with the intentions of the Nine Years of Elementary School Education.

Inhibition of amyloid β aggregation and protective effect on SH-SY5Y cells by triterpenoid saponins from the cactus Polaskia chichipe.

Science.gov (United States)

Fujihara, Koji; Koike, Shin; Ogasawara, Yuki; Takahashi, Kunio; Koyama, Kiyotaka; Kinoshita, Kaoru

2017-07-01

Alzheimer's disease (AD) destroys brain function, especially in the hippocampus, and is a social problem worldwide. A major pathogenesis of AD is related to the accumulation of amyloid beta (Aβ) peptides, resulting in neuronal cell death in the brain. Here, we isolated four saponins (1-4) and elucidated their structures from 1D and 2D NMR and HRFABMS spectral data. The structures of 1 and 2 were determined as new saponins which have cochalic acid as the aglycon, and 3 was determined as a new saponin with oleanolic acid as the aglycon. Compound 4 was confirmed as the known saponin chikusetsusaponin V (=ginsenoside R 0 ). Isolated saponins (1-4) and six previously reported saponins (5-10) were tested for their inhibitory effects of Aβ aggregation and their protective effects on SH-SY5Y cells against Aβ-associated toxicity. As the results, compounds 3 and 4 showed inhibitory effect of Aβ aggregation and compounds 5-8 exerted the protective effects on SH-SY5Y cells against Aβ-associated toxicity. Copyright © 2017 Elsevier Ltd. All rights reserved.

The effects of a three-year smoking prevention programme in secondary schools in Helsinki.

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Vartiainen, Erkki; Pennanen, Marjaana; Haukkala, Ari; Dijk, Froukje; Lehtovuori, Riku; De Vries, Hein

2007-06-01

This study evaluates the effects of a 3-year smoking prevention programme in secondary schools in Helsinki. The study is part of the European Smoking prevention Framework Approach (ESFA), in which Denmark, Finland, the Netherlands, Portugal, Spain and the UK participated. A total of 27 secondary schools in Finland participated in the programme (n = 1821). Schools were randomised into experimental (13) and control groups (14). The programme included 14 information lessons about smoking and refusal skills training. The 3-year smoking prevention programme was also integrated into the standard curriculum. The community-element of the programme included parents, parish confirmation camps and dentists. The schools in the experimental group received the prevention programme and the schools in the control group received the standard health education curriculum. Among baseline never smokers (60.8%), the programme had a significant effect on the onset of weekly smoking in the experimental group [OR = 0.63 (0.45-0.90) P = 0.009] when compared with the control group. Being female, doing poorly at school, having parents and best friends who smoke and more pocket money to spend compared with others were associated with an increased likelihood of daily and weekly smoking onset. These predictors did not have an interaction effect with the experimental condition. This study shows that a school- and community-based smoking prevention programme can prevent smoking onset among adolescents.

A qualitative study to assess school nurses' views on vaccinating 12-13 year old school girls against human papillomavirus without parental consent.

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Stretch, Rebecca; McCann, Rosemary; Roberts, Stephen A; Elton, Peter; Baxter, David; Brabin, Loretta

2009-07-21

In the UK, parental consent for the routine vaccination of 12-13 year olds schoolgirls against human papillomavirus (HPV) is recommended, although legally girls may be able to consent themselves. As part of a vaccine study conducted ahead of the National HPV Vaccine Programme we sought the views of school nurses on vaccinating girls who did not have parental consent. HPV vaccination was offered to all 12 year old girls attending schools in two Primary Care Trusts in Greater Manchester. At the end of the study semi-structured, tape-recorded interviews were conducted with school nurses who had delivered the vaccine (Cervarix). The interview template was based on concepts derived from the Theory of Planned Behaviour. Transcripts were analysed thematically in order to understand school nurses' intentions to implement vaccination based on an assessment of Gillick competency. School nurses knew how to assess the competency of under-16s but were still unwilling to vaccinate if parents had refused permission. If parents had not returned the consent form, school nurses were willing to contact parents, and also to negotiate with parents who had refused consent. They seemed unaware that parental involvement required the child's consent to avoid breaking confidentiality. Nurses' attitudes were influenced by the young appearance and age of the school year group rather than an individual's level of maturity. They were also confused about the legal guidelines governing consent. School nurses acknowledged the child's right to vaccination and strongly supported prevention of HPV infection but ultimately believed that it was the parents' right to give consent. Most were themselves parents and shared other parents' concerns about the vaccine's novelty and unknown long-term side effects. Rather than vaccinate without parental consent, school nurses would defer vaccination. Health providers have a duty of care to girls for whom no parental consent for HPV vaccination has been given

Evaluation of the generation and release of flammable gases in tank 241-SY-101

Energy Technology Data Exchange (ETDEWEB)

Babad, H.; Johnson, G.D.; Lechelt, J.A.; Reynolds, D.A. (Westinghouse Hanford Co., Richland, WA (United States)); Pederson, L.R.; Strachan, D.M. (Pacific Northwest Lab., Richland, WA (United States)); Meisel, D.; Jonah, C. (Argonne National Lab., IL (United States)); Ashby, E.C. (Georgia Inst. of Tech., Atlanta, GA (United States))

1991-11-01

Tank 241-SY-101 is a double shell, high-level waste tank located in the 200 West Area of the Hanford Site. This tank contains about 1 million gallons of waste that was concentrated at the 242-S Evaporator. Shortly after the waste was put in the tank, the waste began to expand because the generation of gases. In 1990 this tank was declared to have an unreviewed safety question because of the periodic release of hydrogen and nitrous oxide. A safety program was established to conduct a characterization of the waste and vented gases and to determine an effective means to prevent the accumulation of flammable gases in the tank dome space and ventilation system. Results of the expanded characterization conducted in fiscal year 1991 are presented. The use of gas chromatographs, mass spectrometers, and hydrogen-specific monitors provided a greater understanding of the vented gases. Additional instrumentation placed in the tank also helped to provide more detailed information on tank temperatures, gas pressure, and gas flow rates. An extensive laboratory study involving the Westinghouse Hanford Company, Pacific Northwest Laboratory, Argonne National Laboratory, and the Georgia Institute of Technology was initiated for the purpose of determining the mechanisms responsible for the generation of various gases. These studies evaluate both radiolytic and thermochemical processes. Results of the first series of experiments are described.

Test plan for qualification testing of the 241-SY-101 Flexible Receiver System

International Nuclear Information System (INIS)

Ritter, G.A.

1994-01-01

A mixer pump was installed in tank 241-SY-101 on July 3, 1993, to support hydrogen mitigation testing. This test mixer pump has proven to be very successful in mitigating the large gas releases, or ''burps,'' from the tank waste. Therefore, a decision has been made to fabricate a spare pump that will be installed upon failure of the existing test pump. Before replacement activities can be initiated, equipment must be available to remove the existing pump. A pump removal system is currently being designed and fabricated that will support the retrieval, transportation and disposal of the existing pump. The Equipment Removal System consists of six major components: the flexible receiver system (FRS), the equipment storage container, the container strongback system, the container transport trailer, the support cranes, and the pump washdown system

Puberty, Health and Sexual Education in Australian Regional Primary Schools: Year 5 and 6 Teacher Perceptions

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Duffy, Bernadette; Fotinatos, Nina; Smith, Amanda; Burke, Jenene

2013-01-01

The research reported in this paper investigates why teachers in regional primary schools in the Ballarat region of Victoria, Australia, are choosing to outsource the teaching of sexuality education. A survey was conducted of 29 Year 5 and Year 6 teachers from local primary schools. The teachers provided information about: their confidence in…

Cardiopulmonary resuscitation training in schools following 8 years of mandating legislation in Denmark

DEFF Research Database (Denmark)

Malta Hansen, Carolina; Zinckernagel, Line; Ersbøll, Annette Kjær

2017-01-01

AND RESULTS: A nationwide cross-sectional survey of Danish school leadership (n=1240) and ninth-grade homeroom teachers (n=1381) was carried out for school year 2013-2014. Qualitative interviews and the Theory of Planned Behavior were used to construct the survey. Logistic regression models were employed...

Cardiopulmonary Resuscitation Training in Schools Following 8 Years of Mandating Legislation in Denmark

DEFF Research Database (Denmark)

Malta Hansen, Carolina; Zinckernagel, Line; Ersbøll, Annette Kjær

2017-01-01

AND RESULTS: A nationwide cross-sectional survey of Danish school leadership (n=1240) and ninth-grade homeroom teachers (n=1381) was carried out for school year 2013-2014. Qualitative interviews and the Theory of Planned Behavior were used to construct the survey. Logistic regression models were employed...

Does school environment affect 11-year-olds' fruit and vegetable intake in Denmark?

DEFF Research Database (Denmark)

Krølner, Rikke; Due, Pernille; Rasmussen, Mette

2009-01-01

It is often found that adolescents eat too little fruit and vegetables. We examined the importance of school for 11-year-olds' daily intake measured by food frequency- and 24-h recall questionnaires in Danish data from the European 2003 Pro Children Survey. Multilevel logistic regression analyses...... > or = 130 g vegetables/day. Most of the total variance in students' intake occurred at the individual level (93-98%). There were larger between-school variations in vegetable intake than in fruit intake. Fruit and vegetable consumption clustered within schools to a larger degree for boys than girls...

Mechanisms of gas generation from simulated SY tank farm wastes: FY 1995 progress report

International Nuclear Information System (INIS)

Barefield, E.K.; Boatright, D.; Deshpande, A.; Doctorovich, F.; Liotta, C.L.; Neumann, H.M.; Seymore, S.

1996-07-01

The objective of this work is to develop a better understanding of the mechanism of formation of flammable gases in the thermal decomposition of metal complexants such as HEDTA and sodium glycolate in simulated SY tank farm waste mixtures. This report summarizes the results of work done at the Georgia Institute of Technology in fiscal year 1995. Topics discussed are (1) long-term studies of the decomposition of HEDTA in simulated waste mixtures under an argon atmosphere at 90 and 120 degrees C, including time profiles for disappearance of HEDTA and appearance of products and the quantitative analysis of the kinetic behavior; (2) considerations of hydroxylamine as an intermediate in the production of nitrogen containing gases by HEDTA decomposition; (3) some thoughts on the revision of the global mechanism for thermal decomposition of HEDTA under argon; (4) preliminary long-term studies of the decomposition of HEDTA in simulated waste under an oxygen atmosphere at 120 degrees C; (5) estimation of the amount of NH 3 in the gas phase above HEDTA reaction mixtures; and (6) further, examination of the interaction of aluminum with nitrite ion using 27 Al NMR spectroscopy. Section 2 of this report describes the work conducted over the last three years at GIT. Section 3 contains a discussion of the kinetic behavior of HEDTA under argon; Section 4 discusses the role of hydroxylamine. Thermal decomposition of HEDTA to ED3A is the subject of Section 5, and decomposition of HEDTA in simulated waste mixtures under oxygen is covered in Section 6. In Section 7 we estimate ammonia in the gas phase; the role of aluminum is discussed in Section 8

Cardiopulmonary Resuscitation Training in Schools Following 8 Years of Mandating Legislation in Denmark

DEFF Research Database (Denmark)

Malta Hansen, Carolina; Zinckernagel, Line; Ersbøll, Annette Kjær

2017-01-01

AND RESULTS: A nationwide cross-sectional survey of Danish school leadership (n=1240) and ninth-grade homeroom teachers (n=1381) was carried out for school year 2013-2014. Qualitative interviews and the Theory of Planned Behavior were used to construct the survey. Logistic regression models were employed...... to identify factors associated with completed CPR training. Information from 63.1% of eligible schools was collected: 49.3% (n=611) of leadership and 48.2% (n=665) of teachers responded. According to teachers, 28.4% (95% CI 25.0% to 32.0%) and 10.3% (95% CI 8.1% to 12.8%) of eligible classes had completed CPR...... and automated external defibrillator training, respectively. Among leadership, 60.2% (95% CI 56.2% to 64.1%) reported CPR training had occurred during the 3 years prior to the survey. Factors associated with completed CPR training included believing other schools were conducting training (odds ratio [OR] 9...

Arctigenin Confers Neuroprotection Against Mechanical Trauma Injury in Human Neuroblastoma SH-SY5Y Cells by Regulating miRNA-16 and miRNA-199a Expression to Alleviate Inflammation.

Science.gov (United States)

Song, Jie; Li, Na; Xia, Yang; Gao, Zhong; Zou, Sa-Feng; Yan, Yu-Hui; Li, Shao-Heng; Wang, Yue; Meng, Ya-Kun; Yang, Jing-Xian; Kang, Ting-Guo

2016-09-01

Mechanical trauma injury is a severe insult to neural cells. Subsequent secondary injury involves the release of inflammatory factors that have dramatic consequences for undamaged cells, leading to normal cell death after the initial injury. The present study investigated the capacity for arctigenin (ARC) to prevent secondary effects and evaluated the mechanism underlying the action of microRNA (miRNA)-199a and miRNA-16 in a mechanical trauma injury (MTI) model using SH-SY5Y cells in vitro. SH-SY5Y cells are often applied to in vitro models of neuronal function and differentiation. Recently, miRNAs have been demonstrated to play a crucial role in NF-κB and cholinergic signaling, which can regulate inflammation. The cell model was established by scratch-induced injury of human SH-SY5Y cells, which mimics the characteristics of MTI. A cell counting kit-8 (CCK-8), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and immunocytochemistry were used to measure cell viability. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the inflammatory cytokine and cholinesterase (CHE) content. The lactate dehydrogenase (LDH) content was measured to assess the degree of cell injury. The mRNA levels were measured by RT-PCR to analyze ARC's mechanism of action. miRNA inhibitors and mimics were used to inhibit and strengthen the expression of miRNAs. Protein expression was detected by western blotting analysis. ARC treatment reduced the TNF-α and IL-6 levels as well as the number of TUNEL+ apoptotic SH-SY5Y cells surrounding the scratch and increased the IL-10 level compared to the controls. ARC attenuated the increase of the cell damage degree and LDH content induced by scratching, indicating increased cell survival. Mechanistic studies showed that ARC upregulated the miRNA-16 and miRNA-199a levels to reduce upstream protein (IKKα and IKKβ) expression and inhibit NF-κB signaling pathway activity; moreover, the increased miRNA-199a suppresses

Mesa Verde--A Year-Round High School. A Descriptive Report of 1980-81.

Science.gov (United States)

Housden, Terry; Holmes, Lynda

Mesa Verde High School was constructed in 1974 and is the last high school built in the San Juan (California) Unified District. It serves students in grades 9 through 12 and has an open enrollment policy. Mesa Verde operates on a year-round schedule with students divided into three tracks. Basically, each track is in session for 9 weeks and then…

Burnout among medical students during the first years of undergraduate school: Prevalence and associated factors.

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Boni, Robson Aparecido Dos Santos; Paiva, Carlos Eduardo; de Oliveira, Marco Antonio; Lucchetti, Giancarlo; Fregnani, José Humberto Tavares Guerreiro; Paiva, Bianca Sakamoto Ribeiro

2018-01-01

To evaluate the prevalence and possible factors associated with the development of burnout among medical students in the first years of undergraduate school. A cross-sectional study was conducted at the Barretos School of Health Sciences, Dr. Paulo Prata. A total of 330 students in the first four years of medical undergraduate school were invited to participate in responding to the sociodemographic and Maslach Burnout Inventory-Student Survey (MBI-SS) questionnaires. The first-year group consisted of 150 students, followed by the second-, third-, and fourth-year groups, with 60 students each. Data from 265 students who answered at least the sociodemographic questionnaire and the MBI-SS were analyzed (response rate = 80.3%). One (n = 1, 0.3%) potential participant viewed the Informed Consent Form but did not agree to participate in the study. A total of 187 students (187/265, 70.6%) presented high levels of emotional exhaustion, 140 (140/265, 52.8%) had high cynicism, and 129 (129/265, 48.7%) had low academic efficacy. The two-dimensional criterion indicated that 119 (44.9%) students experienced burnout. Based on the three-dimensional criterion, 70 students (26.4%) presented with burnout. The year with the highest frequency of affected students for both criteria was the first year (p = 0.001). Personal attributes were able to explain 11% (ΔR = 0.11) of the variability of burnout under the two-dimensional criterion and 14.4% (R2 = 0.144) under the three-dimensional criterion. This study showed a high prevalence of burnout among medical students in a private school using active teaching methodologies. In the first years of graduation, students' personal attributes (optimism and self-perception of health) and school attributes (motivation and routine of the exhaustive study) were associated with higher levels of burnout. These findings reinforce the need to establish preventive measures focused on the personal attributes of first-year students, providing better

Burnout among medical students during the first years of undergraduate school: Prevalence and associated factors

Science.gov (United States)

Paiva, Carlos Eduardo; de Oliveira, Marco Antonio; Lucchetti, Giancarlo; Fregnani, José Humberto Tavares Guerreiro; Paiva, Bianca Sakamoto Ribeiro

2018-01-01

Objective To evaluate the prevalence and possible factors associated with the development of burnout among medical students in the first years of undergraduate school. Method A cross-sectional study was conducted at the Barretos School of Health Sciences, Dr. Paulo Prata. A total of 330 students in the first four years of medical undergraduate school were invited to participate in responding to the sociodemographic and Maslach Burnout Inventory-Student Survey (MBI-SS) questionnaires. The first-year group consisted of 150 students, followed by the second-, third-, and fourth-year groups, with 60 students each. Results Data from 265 students who answered at least the sociodemographic questionnaire and the MBI-SS were analyzed (response rate = 80.3%). One (n = 1, 0.3%) potential participant viewed the Informed Consent Form but did not agree to participate in the study. A total of 187 students (187/265, 70.6%) presented high levels of emotional exhaustion, 140 (140/265, 52.8%) had high cynicism, and 129 (129/265, 48.7%) had low academic efficacy. The two-dimensional criterion indicated that 119 (44.9%) students experienced burnout. Based on the three-dimensional criterion, 70 students (26.4%) presented with burnout. The year with the highest frequency of affected students for both criteria was the first year (p = 0.001). Personal attributes were able to explain 11% (ΔR = 0.11) of the variability of burnout under the two-dimensional criterion and 14.4% (R2 = 0.144) under the three-dimensional criterion. Conclusion This study showed a high prevalence of burnout among medical students in a private school using active teaching methodologies. In the first years of graduation, students’ personal attributes (optimism and self-perception of health) and school attributes (motivation and routine of the exhaustive study) were associated with higher levels of burnout. These findings reinforce the need to establish preventive measures focused on the personal attributes of first-year

Parenting and Temperament Influence on School Success in 9-13 Year Olds.

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Checa, Purificación; Abundis-Gutierrez, Alicia

2017-01-01

Children spend a lot of time with their parents who are the first agents that educate them. The parenting style implemented in the family influences other contexts outside home such as the school. There is evidence that a positive parenting style has an influence on school success. However, there are other variables related to school success, for example, temperament. The influence of parenting decreases with age as children develop abilities to self-regulate without parents' external control. The aim of the present study was to evaluate the contribution of parenting style and temperament in 9-13 years old children on both academic performance and school adjustment skills. Our hypothesis was that not only parenting style is crucial to academic performance and school adjustment, but also temperament plays an important role in them. We used a Parenting Guide line questionnaire to evaluate parenting style, Early Adolescence Temperament Questionnaire-R to evaluate temperament; Health Resources Inventory to assess children's school adjustment, and academic grades, as indicator of academic performance. We were interested in testing whether or not the effect of parenting style on academic performance and school adjustment was mediated by temperament. We found that emotional and behavioral regulation mediates the relation between parenting and academic performance. These findings inform of the relevance of child's temperament on school success. Implications for education are discussed with emphasis on the importance of understanding students' temperament to promote school adjustment and good academic performance.

Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells

Directory of Open Access Journals (Sweden)

Warisa Amornrit

2015-09-01

Full Text Available Amaranthus plants, or spinach, are used extensively as a vegetable and are known to possess medicinal properties. Neuroinflammation and oxidative stress play a major role in the pathogenesis of many neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease. Advanced glycation end-products (AGEs cause cell toxicity in the human neuronal cell line, SH-SY5Y, through an increase in oxidative stress, as shown by reducing cell viability and increasing cell toxicity in a dose-dependent manner. We found that preincubation of SH-SY5Y cells with either petroleum ether, dichloromethane or methanol extracts of A. lividus and A. tricolor dose-dependently attenuated the neuron toxicity caused by AGEs treatment. Moreover, the results showed that A. lividus and A. tricolor extracts significantly downregulated the gene expression of the pro-inflammatory cytokines, TNF-α, IL-1 and IL-6 genes in AGEs-induced cells. We concluded that A. lividus and A. tricolor extracts not only have a neuroprotective effect against AGEs toxicity, but also have anti-inflammatory activity by reducing pro-inflammatory cytokine gene expression. This suggests that Amaranthus may be useful for treating chronic inflammation associated with neurodegenerative disorders.

Effects of Ginkgo biloba extract on the apoptosis of oxygen and glucose-deprived SH-SY5Y cells and its mechanism.

Science.gov (United States)

Ba, Xiao-Hong; Min, Lian-Qiu

2015-01-01

The aim was to observe the effects of the extract of Ginkgo biloba (EGb761) on the apoptosis of oxygen and glucose-deprived (OGD) human neuroblastoma cells (SH-SY5Y) cells and explore its mechanism. SH-SY5Y cells were divided into normal control group, OGD group, OGD for 4 h and EGb761-pretreated groups including very low-concentration (20 μg/ml), low-concentration group (25 μg/ml), moderate-concentration group (50 μg/ml) and high-concentration group (100 μg/ml). Twenty four hours after reoxygenation, cell viability was determined with 3-[4, 5-dimehyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide assay, apoptosis rate was detected with annexin V-fluorescein isothiocyanate/propidium iodide double staining flow cytometry and the protein level of apoptosis-inducing factor (AIF) was observed with immunofluorescence technique in each group. Cell viability was significantly lower in OGD group than in EGb761-pretreated groups, especially in moderate-concentration group (50 μg/ml) (P cells probably through inhibiting AIF nuclear translocation. This study provides a theoretical basis for the application of EGb761 in clinical practice.

Protective Effects of Fisetin Against 6-OHDA-Induced Apoptosis by Activation of PI3K-Akt Signaling in Human Neuroblastoma SH-SY5Y Cells.

Science.gov (United States)

Watanabe, Ryoko; Kurose, Takumi; Morishige, Yuta; Fujimori, Ko

2018-02-01

6-Hydroxydopamine (6-OHDA) induces the production of reactive oxygen species (ROS) that are associated with various neurodegenerative diseases such as Parkinson's disease. 3,3',4',7-Tetrahydroxyflavone (fisetin), a plant flavonoid has a variety of physiological effects such as antioxidant activity. In this study, we investigated the molecular mechanism of the neuroprotective effects of fisetin against 6-OHDA-induced cell death in human neuroblastoma SH-SY5Y cells. 6-OHDA-mediated cell toxicity was reduced in a fisetin concentration-dependent manner. 6-OHDA-mediated elevation of the expression of the oxidative stress-related genes such as hemeoxygenase-1, NAD(P)H dehydrogenase quinone 1, NF-E2-related factor 2, and γ-glutamate-cysteine ligase modifier was suppressed by fisetin. Fisetin also lowered the ratio of the proapoptotic Bax protein and the antiapoptotic Bcl-2 protein in SH-SY5Y cells. Moreover, fisetin effectively suppressed 6-OHDA-mediated activation of caspase-3 and caspase-9, which leads to the cell death, while, 6-OHDA-induced caspase-3/7 activity was lowered. Furthermore, fisetin activated the PI3K-Akt signaling, which inhibits the caspase cascade, and fisetin-mediated inhibition of 6-OHDA-induced cell death was negated by the co-treatment with an Akt inhibitor. These results indicate that fisetin protects 6-OHDA-induced cell death by activating PI3K-Akt signaling in human neuronal SH-SY5Y cells. This is the first report that the PI3K-Akt signaling is involved in the fisetin-protected ROS-mediated neuronal cell death.

Body Mass Index and Sociodemographic Predictors of School Lunch Purchase Behavior during a Year-Long Environmental Intervention in Middle School

Directory of Open Access Journals (Sweden)

Jacey A. Greece

2015-06-01

Full Text Available Modifying the school food environment is on the national agenda as one strategy to improve the nutritional quality of children’s diets. Because few environmental-level interventions have been rigorously evaluated, the evidence base to inform programs and policies is limited. Of concern is the impact that changes to cafeteria offerings will have on participation in school meal programs. This study evaluates school lunch participation in the setting of a year-long middle school cafeteria intervention by examining the association between body mass index (BMI, sociodemographics, and the purchases of school lunch meals. IMOVE meals were healthier choices that met stringent nutritional criteria and were offered alongside standard lunch meals. Students who were overweight had a significantly higher purchase rate for both types of meals compared to those with a healthy BMI. Non-white race, younger age, being male, and low-income status were also significantly associated with participation in school lunch. Results indicate that nutritionally vulnerable students participate in school lunch and are equally likely to buy healthy alternatives or standard meals. This behavioral observation has important implications for school foodservice programs and policies. These results are timely given recent federal legislation to improve the school food environment to influence students’ food choice behaviors.

Body Mass Index and Sociodemographic Predictors of School Lunch Purchase Behavior during a Year-Long Environmental Intervention in Middle School.

Science.gov (United States)

Greece, Jacey A; Kratze, Alyssa; DeJong, William; Cozier, Yvette C; Quatromoni, Paula A

2015-06-10

Modifying the school food environment is on the national agenda as one strategy to improve the nutritional quality of children's diets. Because few environmental-level interventions have been rigorously evaluated, the evidence base to inform programs and policies is limited. Of concern is the impact that changes to cafeteria offerings will have on participation in school meal programs. This study evaluates school lunch participation in the setting of a year-long middle school cafeteria intervention by examining the association between body mass index (BMI), sociodemographics, and the purchases of school lunch meals. IMOVE meals were healthier choices that met stringent nutritional criteria and were offered alongside standard lunch meals. Students who were overweight had a significantly higher purchase rate for both types of meals compared to those with a healthy BMI. Non-white race, younger age, being male, and low-income status were also significantly associated with participation in school lunch. Results indicate that nutritionally vulnerable students participate in school lunch and are equally likely to buy healthy alternatives or standard meals. This behavioral observation has important implications for school foodservice programs and policies. These results are timely given recent federal legislation to improve the school food environment to influence students' food choice behaviors.

Year-round School Makes Good Business Sense, Says This Boardman-Businessman

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Howe, Paul H.

1973-01-01

Argues that year-round schools make good business sense by providing (1) a more efficient use of capital investments, (2) an alleviation of uneconomical and undesirable peaks in working and recreation, and (3) a more sensible way of looking at teacher salaries. (JF)

Changes in the Relation Between Competence Beliefs and Achievement in Math Across Elementary School Years.

Science.gov (United States)

Weidinger, Anne F; Steinmayr, Ricarda; Spinath, Birgit

2018-03-01

Math competence beliefs and achievement are important outcomes of school-based learning. Previous studies yielded inconsistent results on whether skill development, self-enhancement, or reciprocal effects account for the interplay among them. A development-related change in the direction of their relation in the early school years might explain the inconsistency. To test this, 542 German elementary school students (M = 7.95 years, SD = 0.58) were repeatedly investigated over 24 months from Grade 2 to Grade 4. Math competence beliefs declined and had a growing influence on subsequent math grades. This suggests changes in the dominant direction of the relation from a skill development to a reciprocal effects model during elementary school. Findings are discussed with regard to their theoretical and practical implications. © 2017 The Authors. Child Development © 2017 Society for Research in Child Development, Inc.

Does the school food environment influence the dietary behaviours of Norwegian 11-year-olds? The HEIA study.

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Gebremariam, Mekdes K; Andersen, Lene F; Bjelland, Mona; Klepp, Knut-Inge; Totland, Torunn H; Bergh, Ingunn H; Lien, Nanna

2012-07-01

The aim of the study is to investigate the influence of the school food environment on the dietary behaviours of 11-year-old Norwegian children in elementary schools. Baseline data from a school-based intervention study: the Health In Adolescents study was used. A total of 1425 11-year-old children from 35 schools from the eastern part of Norway were included. School administrators provided information on the physical, political, and sociocultural school food environment and students reported their intake of fruits, vegetables, sugar-sweetened beverages (SSB), and snacks. Multilevel modelling was used to assess the school-level variance in dietary behaviours and to investigate the association of school food environmental factors with these dietary behaviours. After adjustment for student characteristics, the school level accounted for a small proportion (1.1%-3.0%) of the variance in the dietary behaviours investigated. None of the investigated school food environmental factors were found to be related to the children's reported intake of fruits, vegetables, snacks or SSB. Most of the variance in the dietary behaviours investigated was at the personal level. Thus in this sample, the investigated school-level factors do not appear to exert a strong influence on the dietary behaviours of children. Longitudinal studies using validated measures of the school food environment are needed.

A Year in Review: The "High School Journal" in 1920

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Job, Jennifer G.

2009-01-01

In 1920, the "High School Journal" had been up and running for a year and cost 15 cents an issue. Robert Goddard was ridiculed by the "New York Times" for predicting space travel (a retraction was printed in 1969), small countries like Ireland and Romania were fighting for their own governments, and American women voted for the…

Protective effects of TRH and its analogues against various cytotoxic agents in retinoic acid (RA)-differentiated human neuroblastoma SH-SY5Y cells.

Science.gov (United States)

Jaworska-Feil, L; Jantas, D; Leskiewicz, M; Budziszewska, B; Kubera, M; Basta-Kaim, A; Lipkowski, A W; Lason, W

2010-12-01

TRH (thyroliberin) and its analogues were reported to possess neuroprotective effects in cellular and animal experimental models of acute and chronic neurodegenerative diseases. In the present study we evaluated effects of TRH and its three stable analogues, montirelin (CG-3703), RGH-2202 and Z-TRH (N-(carbobenzyloxy)-pGlutamyl-Histydyl-Proline) on the neuronally differentiated human neuroblastoma SH-SY5Y cell line, which is widely accepted for studying potential neuroprotectants. We found that TRH and all the tested analogues at concentrations 0.1-50 μM attenuated cell damage induced by MPP(+) (2 mM), 3-nitropropionate (10 mM), hydrogen peroxide (0.5 mM), homocysteine (250 μM) and beta-amyloid (20μM) in retinoic acid differentiated SH-SY5Y cells. Furthermore, we demonstrated that TRH and its analogues decreased the staurosporine (0.5 μM)-induced LDH release, caspase-3 activity and DNA fragmentation, which indicate the anti-apoptotic proprieties of these peptides. The neuroprotective effects of TRH (10 μM) and RGH-2202 (10 μM) on St-induced cell death was attenuated by inhibitors of PI3-K pathway (wortmannin and LY294002), but not MAPK/ERK1/2 (PD98059 and U0126). Moreover, TRH and its analogues at neuroprotective concentrations (1 and 10 μM) increased expression of Bcl-2 protein, as confirmed by Western blot analysis. All in all, these results extend data on neuroprotective properties of TRH and its analogues and provide evidence that mechanism of anti-apoptotic effects of these peptides in SH-SY5Y cell line involves induction of PI3K/Akt pathway and Bcl-2. Furthermore, the data obtained on human cell line with a dopaminergic phenotype suggest potential utility of TRH and its analogues in the treatment of some neurodegenerative diseases including Parkinson's disease. Copyright © 2010 Elsevier Ltd. All rights reserved.

Professional Development and the Teaching Schools Experiment in England: Leadership Challenges in an Alliance's First Year

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Dowling, Simon

2016-01-01

This article reports findings from the first year of a longitudinal, mixed-methods case study of a large teaching school alliance in England. This national initiative is intended to drive improvement at system level by grouping schools around formally designated teaching schools. These "alliances" work collaboratively to share learning,…

Teacher-student interpersonal relationships and academic motivation within one school year : developmental changes and linkage

NARCIS (Netherlands)

Opdenakker, Marie-Christine; Maulana, Ridwan; den Brok, Perry

The present study explored the developmental changes of teacher-student interpersonal relationships as well as that of academic motivation among first-grade secondary school students. In addition, the link between teacher-student interpersonal behaviour and academic motivation across the school year

Evaluation of the School Breakfast Program Pilot Project: Findings from the First Year of Implementation. Nutrition Assistance Program Report Series.

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McLaughlin, Joan E.; Bernstein, Lawrence S.; Crepinsek, May Kay; Daft, Lynn M.; Murphy, J. Michael

In 1998, Congress authorized implementation of a 3-year pilot breakfast program involving 4,300 students in elementary schools in 6 school districts representing a range of economic and demographic characteristics. The program began in the 2000-01 school year. This lengthy report presents the findings from the pilot's first year. The study had two…

Pharmacological Characterization of the Mechanisms Involved in Delayed Calcium Deregulation in SH-SY5Y Cells Challenged with Methadone

Directory of Open Access Journals (Sweden)

Sergio Perez-Alvarez

2012-01-01

Full Text Available Previously, we have shown that SH-SY5Y cells exposed to high concentrations of methadone died due to a necrotic-like cell death mechanism related to delayed calcium deregulation (DCD. In this study, we show that, in terms of their Ca2+ responses to 0.5 mM methadone, SH-SY5Y cells can be pooled into four different groups. In a broad pharmacological survey, the relevance of different Ca2+-related mechanisms on methadone-induced DCD was investigated including extracellular calcium, L-type Ca2+ channels, μ-opioid receptor, mitochondrial inner membrane potential, mitochondrial ATP synthesis, mitochondrial Ca2+/2Na+-exchanger, reactive oxygen species, and mitochondrial permeability transition. Only those compounds targeting mitochondria such as oligomycin, FCCP, CGP 37157, and cyclosporine A were able to amend methadone-induced Ca2+ dyshomeostasis suggesting that methadone induces DCD by modulating the ability of mitochondria to handle Ca2+. Consistently, mitochondria became dramatically shorter and rounder in the presence of methadone. Furthermore, analysis of oxygen uptake by isolated rat liver mitochondria suggested that methadone affected mitochondrial Ca2+ uptake in a respiratory substrate-dependent way. We conclude that methadone causes failure of intracellular Ca2+ homeostasis, and this effect is associated with morphological and functional changes of mitochondria. Likely, this mechanism contributes to degenerative side effects associated with methadone treatment.

Bee venom protects SH-SY5Y human neuroblastoma cells from 1-methyl-4-phenylpyridinium-induced apoptotic cell death.

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Doo, Ah-Reum; Kim, Seung-Nam; Kim, Seung-Tae; Park, Ji-Yeun; Chung, Sung-Hyun; Choe, Bo-Young; Chae, Younbyoung; Lee, Hyejung; Yin, Chang-Shik; Park, Hi-Joon

2012-01-06

Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by progressive selective loss of dopaminergic neurons in the substantia nigra. Recently, bee venom was reported to protect dopaminergic neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced mice PD model, however, the underlying mechanism is not fully understood. The objective of the present study is to investigate the neuroprotective mechanism of bee venom against Parkinsonian toxin, 1-methyl-4-phenylpyridine (MPP(+)), in SH-SY5Y human neuroblastoma cells. Our results revealed that bee venom pretreatment (1-100 ng/ml) increased the cell viability and decreased apoptosis assessed by DNA fragmentation and caspase-3 activity assays in MPP(+)-induced cytotoxicity in SH-SY5Y cells. Bee venom increased the anti-apoptotic Bcl-2 expression and decreased the pro-apoptotic Bax, cleaved PARP expressions. In addition, bee venom prevented the MPP(+)-induced suppression of Akt phosphorylation, and the neuroprotective effect of bee venom against MPP(+)-induced cytotoxicity was inhibited by a phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002. These results suggest that the anti-apoptotic effect of bee venom is mediated by the cell survival signaling, the PI3K/Akt pathway. These results provide new evidence for elucidating the mechanism of neuroprotection of bee venom against PD. Copyright © 2011 Elsevier B.V. All rights reserved.

Does a selection interview predict year 1 performance in dental school?

Science.gov (United States)

McAndrew, R; Ellis, J; Valentine, R A

2017-05-01

It is important for dental schools to select students who will complete their degree and progress on to become the dentists of the future. The process should be transparent, fair and ethical and utilise selection tools that select appropriate students. The interview is an integral part of UK dental schools student selection procedures. This study was undertaken in order to determine whether different interview methods (Cardiff with a multiple mini interview and Newcastle with a more traditional interview process) along with other components used in selection predicted academic performance in students. The admissions selection data for two dental schools (Cardiff and Newcastle) were collected and analysed alongside student performance in academic examinations in Year 1 of the respective schools. Correlation statistics were used to determine whether selection tools had any relevance to academic performance once students were admitted to their respective Universities. Data was available for a total of 177 students (77 Cardiff and 100 Newcastle). Examination performance did not correlate with admission interview scores at either school; however UKCAT score was linked to poor academic performance. Although interview methodology does not appear to correlate with academic performance it remains an integral and very necessary part of the admissions process. Ultimately schools need to be comfortable with their admissions procedures in attracting and selecting the calibre of students they desire. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Dental erosion in 12-year-old school children living in Jakarta

Science.gov (United States)

Septalita, A.; Bahar, A.; Agustanti, A.; Rahardjo, A.; Maharani, D. A.; Rosalien, R.

2017-08-01

This study assesses the dental erosion status of 12-year-old Indonesian children and studies the determinants of dental erosion of these children. The survey was performed in 2016 with ethics approval. A multistage cluster proportional to size random sampling method was adopted to select 12-year-old children in 24 primary schools in Jakarta. The parents were asked to complete a self-administered questionnaire concerning their children’s diet and oral health habits. The children were examined by a single calibrated examiner. Detection of dental erosion followed basic erosive wear examination (BEWE) criteria. A total of 487 children participated in the survey. Most children (88%) had at least some signs of erosion (BEWE > 0), with dentin being involved in 50% of the cases (BEWE = 2). Dental erosion was significantly related to gender, the frequencies of citric tea consumption, parent’s dental knowledge, father’s education, and dental caries (OR = 3.148). The 12-year-old Indonesian school children who lived in Jakarta had signs of erosion, although severe erosion was not found. Screening programs should be provided to identify risk groups so early preventive measures can be taken.

Optimization of trehalose production by a novel strain Brevibacterium sp. SY361.

Science.gov (United States)

Wang, Lei; Huang, Rui; Gu, Guanbin; Fang, Hongying

2008-10-01

Trehalose production by a novel strain of Brevibacterium sp. SY361 was optimized in submerged fermentation. Different chemical and physical parameters such as carbon and nitrogen sources, inoculum level, initial pH, incubation temperature, aeration and time-course of fermentation, were studied in order to increase trehalose productivity. An optimal production medium containing 3% (w/v) glucose, 0.9% (v/v) corn steep liquor, 0.5% (w/v) KH(2)PO(4) and 0.4% (w/v) MgSO(4).7 H(2)O was found suitable for trehalose production. An optimal volume of medium in a 500 ml flask was 80 ml. The optimal levels of other parameters were 4.0% (v/v) of inoculum, initial pH of 6.0, incubation temperature of 28-32 degrees C and time-course of 60 h. Optimized parameters gave a maximum trehalose of 12.2 mg/ml with a conversion rate of 58.4%. (c) 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Caries risk assessment among 12-13 year old school-going children of government and private schools of Tirupur district, Tamil Nadu.

Science.gov (United States)

Mitha, Madhu M; Nijesh, J E; Chaly, Preetha Elizabeth; Priyadharshini, Indra; Junaid, Mohammed; Vaishnavi, S

2016-01-01

Dental caries is as ancient as humankind and has the longest association with the dental profession, an association that is punctuated with agony and ecstasy. The agonizing fact is that despite several efforts toward total eradication, this disease is still prevalent. Nevertheless, an ecstatic success of the profession is the global decline in the incidence compared to the yesteryears' epidemics. Hence, predicting dental caries earlier is a boon. One such model to predict is cariogram developed by Bratthall in 1996. The aim of this study was to assess the caries risk among 12-13 year old school-going children of government and private schools of Tirupur district in Tamil Nadu using cariogram computer model. A cross-sectional survey was carried out among 136 study subjects of 12-13 year of age, who fulfilled the inclusion and exclusion criteria. Data were collected using a predesigned questionnaire and scored according to a standardized protocol. The Chi-square test was used to find differences between caries-related factors and cariogram group. The correlation was acquired using Spearman's correlation. Government school study subjects had 56% of chance of avoiding caries whereas the private school study subjects had 66% of chance of avoiding caries in future and the differences were statistically significant (P = 0.001). A negative correlation was observed between the chance to avoid dental caries and cariogram sectors. The majority of the study subjects from government school belonged to medium-risk category and private school subjects belonged to low-risk category which inferred that private school students have high chance to avoid dental caries compared to government study subjects.

Functional performance of school children diagnosed with developmental delay up to two years of age

Science.gov (United States)

Dornelas, Lílian de Fátima; Magalhães, Lívia de Castro

2016-01-01

Abstract Objective: To compare the functional performance of students diagnosed with developmental delay (DD) up to two years of age with peers exhibiting typical development. Methods: Cross-sectional study with functional performance assessment of children diagnosed with DD up to two years of age compared to those with typical development at seven to eight years of age. Each group consisted of 45 children, selected by non-random sampling, evaluated for motor skills, quality of home environment, school participation and performance. ANOVA and the Binomial test for two proportions were used to assess differences between groups. Results: The group with DD had lower motor skills when compared to the typical group. While 66.7% of children in the typical group showed adequate school participation, receiving aid in cognitive and behavioral tasks similar to that offered to other children at the same level, only 22.2% of children with DD showed the same performance. Although 53.3% of the children with DD achieved an academic performance expected for the school level, there were limitations in some activities. Only two indicators of family environment, diversity and activities with parents at home, showed statistically significant difference between the groups, with advantage being shown for the typical group. Conclusions: Children with DD have persistent difficulties at school age, with motor deficit, restrictions in school activity performance and low participation in the school context, as well as significantly lower functional performance when compared to children without DD. A systematic monitoring of this population is recommended to identify needs and minimize future problems. PMID:26553573

Functional performance of school children diagnosed with developmental delay up to two years of age

Directory of Open Access Journals (Sweden)

Lílian de Fátima Dornelas

2016-03-01

Full Text Available Abstract Objective: To compare the functional performance of students diagnosed with developmental delay (DD up to two years of age with peers exhibiting typical development. Methods: Cross-sectional study with functional performance assessment of children diagnosed with DD up to two years of age compared to those with typical development at seven to eight years of age. Each group consisted of 45 children, selected by non-random sampling, evaluated for motor skills, quality of home environment, school participation and performance. ANOVA and the Binomial test for two proportions were used to assess differences between groups. Results: The group with DD had lower motor skills when compared to the typical group. While 66.7% of children in the typical group showed adequate school participation, receiving aid in cognitive and behavioral tasks similar to that offered to other children at the same level, only 22.2% of children with DD showed the same performance. Although 53.3% of the children with DD achieved an academic performance expected for the school level, there were limitations in some activities. Only two indicators of family environment, diversity and activities with parents at home, showed statistically significant difference between the groups, with advantage being shown for the typical group. Conclusions: Children with DD have persistent difficulties at school age, with motor deficit, restrictions in school activity performance and low participation in the school context, as well as significantly lower functional performance when compared to children without DD. A systematic monitoring of this population is recommended to identify needs and minimize future problems.

[Preschool familial environment and academic difficulties: A 10-year follow-up from kindergarten to middle school].

Science.gov (United States)

Câmara-Costa, H; Pulgar, S; Cusin, F; Dellatolas, G

2016-02-01

The persistence of academic difficulties from childhood through adulthood has led researchers to focus on the identification of the early factors influencing children's subsequent achievement in order to improve the efficient screening of children who might be at risk of school failure. The foundations of academic achievement can be accurately traced back to the preschool years prior to children's entry in formal schooling and are largely influenced by environmental determinants. Importantly, some environmental conditions act as early risk factors undermining children's later academic achievement due to the well-established relation between underachievement and exposure to moderate to high levels of environmental risk. In the present study, we aimed to investigate the longitudinal effects of environment-level factors (sociodemographic and family characteristics) and early risk exposure at kindergarten on children's subsequent academic achievement at the end of middle school (grade 9). The sample of analysis comprised 654 kindergarteners aged 5-6 years (2001-2002 school year) followed through the end of middle school when they were aged 14-15 years (2010-2011 school year). At kindergarten, assessment included questionnaire-based measures of sociodemographic and family background characteristics. These included an original set of information pertaining to family background including parental nationality, education level, history of reading difficulties, type of early childcare, family situation, family size, and language-based bedtime routines, as well as individual-level factors such as children's first language, medical history, language delay, birth weight, age of walking onset, and gestation period. At grade 9, outcome measures were composed of children's results in the national evaluations performed at the end of middle school ("Diplôme National du Brevet"), or history of repetition for a second year of the same class. The results indicated that all family

Estimating dim light melatonin onset (DLMO) phase in adolescents using summer or school-year sleep/wake schedules.

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Crowley, Stephanie J; Acebo, Christine; Fallone, Gahan; Carskadon, Mary A

2006-12-01

This analysis examined associations between the salivary dim light melatonin onset (DLMO) phase and self-selected sleep/ wake schedules in groups of children and adolescents during summer vacation and during the school year to determine the degree to which sleep/wake patterns can estimate salivary DLMO phase. Participants slept at home on self-selected schedules for 5 consecutive nights and reported their bedtime and wake-up time via daily telephone messages. Salivary melatonin was sampled in the laboratory on one evening every 30 minutes in dim light (females) contributed 149 DLMO phase and sleep/wake pattern measures while on a school year schedule ("school group"). A separate group, ages 9 to 16 years (mean age = 13.1, SD = 1.3 years, 30 males, 29 females) contributed 59 DLMO phase and sleep/wake pattern measures while on a summer schedule ("summer group"). Bedtime, midsleep time, and wake-up time were positively correlated with DLMO phase in both groups. Although all correlation coefficients for the summer group were statistically greater compared to the school group, the regression equations predicted DLMO phase within +/- 1 hour of the measured DLMO phase in approximately 80% for both groups. DLMO phase can be estimated using self-selected sleep/wake patterns during the school year or summer vacation in healthy children and adolescents.

A Year after Columbine: Public Looks to Parents More than Schools To Prevent Violence.

Science.gov (United States)

Pew Research Center for the People and the Press, Washington, DC.

An April 2000 telephone survey queried a nationwide sample of 1,000 adults, including 283 parents of children ages 5 to 17 years, concerning school violence and other issues in the news. The vast majority of those surveyed said they believe it is the responsibility of parents to ensure that school shootings, such as occurred at Columbine High…

Sexual behavior, depressive feelings, and suicidality among Estonian school children aged 13 to 15 years.

Science.gov (United States)

Heidmets, L; Samm, A; Sisask, M; Kõlves, K; Aasvee, K; Värnik, A

2010-01-01

The present paper is based on a WHO Collaborative Cross-National Study "Health Behavior in School-Aged Children (HBSC)." It aimed at describing and analyzing how the sexual behaviors of 13- to 15-year-old Estonian school children were associated with self-reported depressive feelings and suicidality. Distinctive behavioral traits in relation to age of first sexual intercourse were also investigated. Self-reported questionnaires from school children (n = 3,055) were analyzed. In total, 15.2% of school children reported being nonvirgin. Among 13-year-olds, 2.9% of girls and 6.8% of boys were nonvirgins. Approximately 25% of the 15-year-old girls and boys were nonvirgins. The likelihood of depressive feelings and suicidal ideation increased significantly in both genders with loss of virginity. Boys who had lost their virginity at 13 years or younger were 4.2 times more likely to have suicidal thoughts; comparable girls were 7.8 times more likely to have suicidal thoughts. Compared to virgins, youths who had lost their virginity reported poor self-assessed health and more risk behaviors in themselves and their peers. Experiences of sexual intercourse increased the odds ratios for depressive feelings and suicidality. The earlier sexual intercourse was initiated, the greater were the odds of lower mental well-being. Risk behaviors emerged as a complex phenomenon requiring complex prevention.

Prevalence of Dental Caries in 5 – 6 Years and 12 – 13 Years Age Group of School Children of Kathmandu Valley

Directory of Open Access Journals (Sweden)

B Subedi

2011-12-01

Full Text Available Introduction: Dental caries is one of the most common conditions affecting the general health of children. The present study was carried out among school children of Kathmandu valley to determine the prevalence of dental caries in two age groups. Methods: The study was conducted from December 2007 to May 2008. The age of the school children of the study was divided into two group: 5 - 6 years and 12 - 13 years. A stratifi ed cluster sampling with proportional allocation was used while grouping the subjects. The dental status examination was done with the help of trained dentists. Decayed, missed and fi lled teeth index and decayed, missed and fi lled surfaces index (dmft for primary dentition and DMFT for permanent dentition were used as the standard tools for the determination of prevalence. Results: A total of 638 students (325 of age group 12 - 13 years and 313 of age group 5 - 6 years from 30 different schools of the Kathmandu valley were included in the study. The caries status was found higher in the age group of 5 - 6 years than in the 12 - 13 years and it was found to be statistically signifi cant (p < 0.001. The dmfs and caries percentage of the age group 5 - 6 years and the DMFS and caries percent of the 12 - 13 years was found to be 3.79, 69 % and 1.6, 53.23 % respectively. The dmft/dmfs value was found to be signifi cant according to the districts in the 5 - 6 years age group whereas the DMFS was found statistically signifi cant among the sexes of the 12 - 13 years age group. Conclusions: The caries percentage was found to be above the recommended level of the World Health Organization. However, the DMFS and DMFT values were within the WHO level. Keywords: Children, dental caries, DMF index.

Dental erosion among 12-14 year old school children in Khartoum: a pilot study.

Science.gov (United States)

El Karim, I A; Sanhouri, N M; Hashim, N T; Ziada, H M

2007-09-01

To investigate dental erosion among 12-14 year old Sudanese school children and evaluate the associated risk factors. Cross sectional survey in secondary schools in Khartoum city, Sudan. A sample of 157 school children was obtained from both private and public schools. Erosion on the labial and palatal surfaces of maxillary incisors was measured by criterion based on the Smith and Knight Tooth Wear Index. Dietary intake and other related factors were assessed using a questionnaire. The overall erosion prevalence in this group was 66.9%, of which 45.2% was mild and 21.7% was moderate erosion. A strong association was found between erosion and private schooling (higher socioeconomic groups), carbonated drinks, herbal hibiscus drink and traditional acidic food consumption. There was a high prevalence of dental erosion among Sudanese school children which was mild to moderate in severity and was strongly associated with acidic dietary intake

Astronomy in the early years of elementary education: a partnership between university and school

Science.gov (United States)

Barai, A.; Carvalho Neto, J. T.; Garrido, D.; Ityanagui, G.; Navi, M.

2016-12-01

This paper describes the interaction and partnership experience between a school and one of the Federal University of São Carlos (UFSCar)campi, both located in Araras, SP, aiming to teach and promote astronomy and astronautics knowledge among students of the first five years of Elementary Education. This initiative made use of Brazilian Olympiad of Astronomy and Astronautics as a motivating event for the theme exploration. The actions were divided into two fronts: an improvement course for the school teachers conducted by university professors and lectures for students by UFSCar students under the guidance of university teachers and the school coordinators. By the observed results, we noticed the importance of narrowing the distance school-university, promoting learning for both institutions and helping to raise the level of education from elementary school to college.

GeoFORCE Alaska: Four-Year Field Program Brings Rural Alaskan High School Students into the STEM Pipeline

Science.gov (United States)

Fowell, S. J.; Rittgers, A.; Stephens, L.; Hutchinson, S.; Peters, H.; Snow, E.; Wartes, D.

2016-12-01

GeoFORCE Alaska is a four-year, field-based, summer geoscience program designed to raise graduation rates in rural Alaskan high schools, encourage participants to pursue college degrees, and increase the diversity of Alaska's technical workforce. Residents of predominantly Alaska Native villages holding degrees in science, technology, engineering, or math (STEM) bring valuable perspectives to decisions regarding management of cultural and natural resources. However, between 2010 and 2015 the average dropout rate for students in grades 7-12 was 8.5% per year in the North Slope School District and 7% per year in the Northwest Arctic School District. 2015 graduation rates were 70% and 75%, respectively. Statewide statistics highlight the challenge for Alaska Native students. During the 2014-2015 school year alone 37.6% of Alaska Native students dropped out of Alaskan public schools. At the college level, Alaska Native students are underrepresented in University of Alaska Fairbanks (UAF) science departments. Launched in 2012 by UAF in partnership with the longstanding University of Texas at Austin program, GeoFORCE applies the cohort model, leading the same group of high school students on geological field academies during four consecutive summers. Through a combination of active learning, teamwork, and hands-on projects at spectacular geological locations, students gain academic skills and confidence that facilitate high school and college success. To date, GeoFORCE Alaska has recruited two cohorts. 78% of these students identify as Alaska Native, reflecting community demographics. The inaugural cohort of 18 students from the North Slope Borough completed the Fourth-Year Academy in summer 2015. 94% of these students graduated from high school, at least 72% plan to attend college, and 33% will major in geoscience. A second cohort of 34 rising 9th and 10th graders entered the program in 2016. At the request of corporate sponsors, this cohort was recruited from both the

Mastery of Fundamental Movement Skills among 6-Year-Old Flemish Pre-School Children

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Vandaele, Bart; Cools, Wouter; de Decker, Steve; de Martelaer, Kristine

2011-01-01

The purpose of this study was to assess mastery of Fundamental Movement Skills (FMS) in 6- to 6.5-year-old Flemish pre-school children. The subjects were 236 6-year-old children (138 boys, 98 girls; mean age 6 years 2.4 months, SD 2.4). Children were individually assessed with the Motoriktest fur Vier- bis Sechsjahrige Kinder (MOT 4-6) in four…

Individual and maternal determinants of self-reported dental health among Turkish school children aged 10-12 years

DEFF Research Database (Denmark)

Cinar, A B; Kosku, N; Sandalli, N

2008-01-01

To assess the influence of maternal and individual characteristics on self-reported dental health of Turkish school children aged 10-12 years with different socio-economic backgrounds.......To assess the influence of maternal and individual characteristics on self-reported dental health of Turkish school children aged 10-12 years with different socio-economic backgrounds....

An Investigation of Emotional Skills of Six-Year-Old Children Attending Nursery School According to Some Variables

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Durmusoglu-Saltali, Neslihan; Arslan, Emel

2011-01-01

The purpose of this study is for the emotional skills of six-year-old children attending nursery school according to some variables. The participants were 306 (135 girls and 171 boys) six-year-old children attending nursery school. Data were collected from Assessment of Children's Emotional Skills and personal information form. In order to analyze…

New Mexico High School Supercomputing Challenge, 1990--1995: Five years of making a difference to students, teachers, schools, and communities. Progress report

Energy Technology Data Exchange (ETDEWEB)

Foster, M.; Kratzer, D.

1996-02-01

The New Mexico High School Supercomputing Challenge is an academic program dedicated to increasing interest in science and math among high school students by introducing them to high performance computing. This report provides a summary and evaluation of the first five years of the program, describes the program and shows the impact that it has had on high school students, their teachers, and their communities. Goals and objectives are reviewed and evaluated, growth and development of the program are analyzed, and future directions are discussed.

The effects of Nordic school meals on concentration and school performance in 8- to 11-year-old children in the OPUS School Meal Study: a cluster-randomised, controlled, cross-over trial.

Science.gov (United States)

Sørensen, Louise B; Dyssegaard, Camilla B; Damsgaard, Camilla T; Petersen, Rikke A; Dalskov, Stine-Mathilde; Hjorth, Mads F; Andersen, Rikke; Tetens, Inge; Ritz, Christian; Astrup, Arne; Lauritzen, Lotte; Michaelsen, Kim F; Egelund, Niels

2015-04-28

It is widely assumed that nutrition can improve school performance in children; however, evidence remains limited and inconclusive. In the present study, we investigated whether serving healthy school meals influenced concentration and school performance of 8- to 11-year-old Danish children. The OPUS (Optimal well-being, development and health for Danish children through a healthy New Nordic Diet) School Meal Study was a cluster-randomised, controlled, cross-over trial comparing a healthy school meal programme with the usual packed lunch from home (control) each for 3 months (NCT 01457794). The d2 test of attention, the Learning Rating Scale (LRS) and standard tests on reading and mathematics proficiency were administered at baseline and at the end of each study period. Intervention effects were evaluated using hierarchical mixed models. The school meal intervention did not influence concentration performance (CP; primary outcome, n 693) or processing speed; however, the decrease in error percentage was 0·18 points smaller (Pschool year. The percentage of correct sentences also improved (Pperformance or outcomes from the LRS. In conclusion, school meals did not affect CP, but improved reading performance, which is a complex cognitive activity that involves inference, and increased errors related to impulsivity and inattention. These findings are worth examining in future trials.

B and V photoelectric photometry and light curve solution of the interacting binary systems HI Puppis, BL Eridani and SY Horologii

International Nuclear Information System (INIS)

Kern, J.R.

1985-01-01

The first photoelectric observations of the eclipsing binary systems HI Puppis, BL Eridani, and SY Horologii were obtained and complete B and V light curves are presented. Improved light elements were derived for all three systems. The light curves of HI Puppis were analyzed by the three different computer models of Wilson and Devinney (1971), Binnendijk (1977), and Wood (1971) to get the geometrical and photometric elements of the system. HI Puppis, whose light curves show a total eclipse at secondary minimum, is shown to be a classic A-Type W Ursae Majoris system. The geometrical and photometric elements of BL Eridani were obtained from the analysis of the light curves using the method of Wilson and Devinney. The asymmetric nature of the light curve is treated as being due to the presence of an underluminous starspot on the primary component. The light curves of BL Eri show secondary eclipse to be total. The system is found to be a near contact system with the primary component filling its Roche lobe and the secondary nearly filling its lobe with a fill-out ratio of approximately 1.0. The light curves of SY Horologii were also subjected to the model of Wilson and Devinney. Both primary and secondary eclipses are partial

The English proficiency and academic language skills of Australian bilingual children during the primary school years.

Science.gov (United States)

Dennaoui, Kamelia; Nicholls, Ruth Jane; O'Connor, Meredith; Tarasuik, Joanne; Kvalsvig, Amanda; Goldfeld, Sharon

2016-04-01

Evidence suggests that early proficiency in the language of school instruction is an important predictor of academic success for bilingual children. This study investigated whether English-proficiency at 4-5 years of age predicts academic language and literacy skills among Australian bilingual children at 10-11 years of age, as part of the Longitudinal Study of Australian Children ( LSAC, 2012 ). The LSAC comprises a nationally representative clustered cross-sequential sample of Australian children. Data were analysed from a sub-sample of 129 bilingual children from the LSAC Kindergarten cohort (n = 4983), for whom teachers completed the Australian Early Development Index (AEDI) checklist (a population measure of early childhood development) and the Academic Rating Scale (ARS) language and literacy subscale. Linear regression analyses revealed that bilingual children who commenced school with stronger English proficiency had higher academic language and literacy scores at the end of primary school (β = 0.45). English proficiency remained a significant predictor, even when accounting for gender and socio-economic disadvantage (β = 0.38). The findings indicate that bilingual children who begin school without English proficiency are at risk of difficulties with academic language and literacy, even after 6 years of schooling. Risk factors need to be identified so early support can be targeted towards the most vulnerable children.

Teacher-Student Interpersonal Relationships and Academic Motivation within One School Year: Developmental Changes and Linkage

Science.gov (United States)

Opdenakker, Marie-Christine; Maulana, Ridwan; den Brok, Perry

2012-01-01

The present study explored the developmental changes of teacher-student interpersonal relationships as well as that of academic motivation among first-grade secondary school students. In addition, the link between teacher-student interpersonal behaviour and academic motivation across the school year was investigated. The data were collected 5…

Digging up Von Békésy: Funneling of Touches around the Body

Directory of Open Access Journals (Sweden)

Lisa M Pritchett

2011-10-01

Full Text Available We investigated a technique that can be used to place tactile stimuli on a continuum between two points using two tactors placed several centimeters apart. As von Békésy first described in 1959, when two pieces of skin several centimeters apart are simultaneously stimulated, a single touch is perceived at an intermediate location. When the relative intensity of the vibration of the two tactors is slowly adjusted from 1:0 to 0:1 a sensation is created of a touch moving across the skin. This has come to be known as the funneling illusion. We have characterized the funneling illusion on a variety of body parts including the hand, arm, wrist, head, torso, and legs. We show that the illusion is robust to a variety of vibration parameters, and demonstrate how it may be used for within modality touch discrimination and adjustment procedures.

Rosiglitazone protects human neuroblastoma SH-SY5Y cells against acetaldehyde-induced cytotoxicity

International Nuclear Information System (INIS)

Jung, Tae Woo; Lee, Ji Young; Shim, Wan Sub; Kang, Eun Seok; Kim, Soo Kyung; Ahn, Chul Woo; Lee, Hyun Chul; Cha, Bong Soo

2006-01-01

Acetaldehyde, an inhibitor of mitochondrial function, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome with elevation of the intracellular reactive oxygen species level and apoptosis. Rosiglitazone, a peroxisome proliferator-activated receptor-γ agonist, has been known to show various non-hypoglycemic effects, including anti-inflammatory, anti-atherogenic, and anti-apoptotic. In this study, we investigated the protective effects of rosiglitazone on acetaldehyde-induced apoptosis in human neuroblastoma SH-SY5Y cells and attempted to examine its mechanism. Acetaldehyde-induced apoptosis was moderately reversed by rosiglitazone treatment. Our results suggest that the protective effects of rosiglitazone on acetaldehyde-induced apoptosis may be ascribed to ability to induce the expression of anti-oxidant enzymes and to regulate Bcl-2 and Bax expression. These data indicate that rosiglitazone may provide a useful therapeutic strategy for the prevention of progressive neurodegenerative disease such as Parkinson's disease

A qualitative study to assess school nurses' views on vaccinating 12–13 year old school girls against human papillomavirus without parental consent

Directory of Open Access Journals (Sweden)

Baxter David

2009-07-01

Full Text Available Abstract Background In the UK, parental consent for the routine vaccination of 12–13 year olds schoolgirls against human papillomavirus (HPV is recommended, although legally girls may be able to consent themselves. As part of a vaccine study conducted ahead of the National HPV Vaccine Programme we sought the views of school nurses on vaccinating girls who did not have parental consent. Methods HPV vaccination was offered to all 12 year old girls attending schools in two Primary Care Trusts in Greater Manchester. At the end of the study semi-structured, tape-recorded interviews were conducted with school nurses who had delivered the vaccine (Cervarix™. The interview template was based on concepts derived from the Theory of Planned Behaviour. Transcripts were analysed thematically in order to understand school nurses' intentions to implement vaccination based on an assessment of Gillick competency. Results School nurses knew how to assess the competency of under-16s but were still unwilling to vaccinate if parents had refused permission. If parents had not returned the consent form, school nurses were willing to contact parents, and also to negotiate with parents who had refused consent. They seemed unaware that parental involvement required the child's consent to avoid breaking confidentiality. Nurses' attitudes were influenced by the young appearance and age of the school year group rather than an individual's level of maturity. They were also confused about the legal guidelines governing consent. School nurses acknowledged the child's right to vaccination and strongly supported prevention of HPV infection but ultimately believed that it was the parents' right to give consent. Most were themselves parents and shared other parents' concerns about the vaccine's novelty and unknown long-term side effects. Rather than vaccinate without parental consent, school nurses would defer vaccination. Conclusion Health providers have a duty of care to

[Functional performance of school children diagnosed with developmental delay up to two years of age].

Science.gov (United States)

Dornelas, Lílian de Fátima; Magalhães, Lívia de Castro

2016-01-01

To compare the functional performance of students diagnosed with developmental delay (DD) up to two years of age with peers exhibiting typical development. Cross-sectional study with functional performance assessment of children diagnosed with DD up to two years of age compared to those with typical development at seven to eight years of age. Each group consisted of 45 children, selected by non-random sampling, evaluated for motor skills, quality of home environment, school participation and performance. ANOVA and the Binomial test for two proportions were used to assess differences between groups. The group with DD had lower motor skills when compared to the typical group. While 66.7% of children in the typical group showed adequate school participation, receiving aid in cognitive and behavioral tasks similar to that offered to other children at the same level, only 22.2% of children with DD showed the same performance. Although 53.3% of the children with DD achieved an academic performance expected for the school level, there were limitations in some activities. Only two indicators of family environment, diversity and activities with parents at home, showed statistically significant difference between the groups, with advantage being shown for the typical group. Children with DD have persistent difficulties at school age, with motor deficit, restrictions in school activity performance and low participation in the school context, as well as significantly lower functional performance when compared to children without DD. A systematic monitoring of this population is recommended to identify needs and minimize future problems. Copyright © 2015 Sociedade de Pediatria de São Paulo. Publicado por Elsevier Editora Ltda. All rights reserved.

Effect of Religious Attendance on Years of Schooling in the USA

Science.gov (United States)

Mohanty, Madhu S.

2016-01-01

Using data from the USA, the study demonstrates that an individual's completed years of schooling later in life is positively related to his/her frequency of religious attendance during youth. Using the propensity score matching technique, the study shows that this relationship is causal. This conclusion remains valid for youths of different…

Medical school attrition-beyond the statistics a ten year retrospective study.

Science.gov (United States)

Maher, Bridget M; Hynes, Helen; Sweeney, Catherine; Khashan, Ali S; O'Rourke, Margaret; Doran, Kieran; Harris, Anne; Flynn, Siun O'

2013-01-31

Medical school attrition is important--securing a place in medical school is difficult and a high attrition rate can affect the academic reputation of a medical school and staff morale. More important, however, are the personal consequences of dropout for the student. The aims of our study were to examine factors associated with attrition over a ten-year period (2001-2011) and to study the personal effects of dropout on individual students. The study included quantitative analysis of completed cohorts and qualitative analysis of ten-year data. Data were collected from individual student files, examination and admission records, exit interviews and staff interviews. Statistical analysis was carried out on five successive completed cohorts. Qualitative data from student files was transcribed and independently analysed by three authors. Data was coded and categorized and key themes were identified. Overall attrition rate was 5.7% (45/779) in 6 completed cohorts when students who transferred to other medical courses were excluded. Students from Kuwait and United Arab Emirates had the highest dropout rate (RR = 5.70, 95% Confidence Intervals 2.65 to 12.27;p psychological morbidity in 40% (higher than other studies). Qualitative analysis revealed recurrent themes of isolation, failure, and despair. Student Welfare services were only accessed by one-third of dropout students. While dropout is often multifactorial, certain red flag signals may alert us to risk of dropout including non-EU origin, academic struggling, absenteeism, social isolation, depression and leave of absence. Psychological morbidity amongst dropout students is high and Student Welfare services should be actively promoted. Absenteeism should prompt early intervention. Behind every dropout statistic lies a personal story. All medical schools have a duty of care to support students who leave the medical programme.

Chrysotoxine, a novel bibenzyl compound selectively antagonizes MPP⁺, but not rotenone, neurotoxicity in dopaminergic SH-SY5Y cells.

Science.gov (United States)

Song, Ju-Xian; Shaw, Pang-Chui; Wong, Ngok-Shun; Sze, Cho-Wing; Yao, Xin-Sheng; Tang, Chi-Wai; Tong, Yao; Zhang, Yan-Bo

2012-07-11

Chrysotoxine is a naturally occurring bibenzyl compound found in medicinal Dendrobium species. We previously reported that chrysotoxine structure-specifically suppressed 6-hydroxydopamine (6-OHDA)-induced dopaminergic cell death. Whether chrysotoxine and other structurally similar bibenzyl compounds could also inhibit the neurotoxicity of 1-methyl-4-phenyl pyridinium (MPP(+)) and rotenone has not been investigated. We showed herein that chrysotoxine inhibited MPP(+), but not rotenone, induced dopaminergic cell death in SH-SY5Y cells. The overproduction of reactive oxygen species (ROS), mitochondrial dysfunction as indexed by the decrease in membrane potential, increase in calcium concentration and NF-κB activation triggered by MPP(+) were blocked by chrysotoxine pretreatment. The imbalance between the pro-apoptotic signals (Bax, caspase-3, ERK and p38 MAPK) and the pro-survival signals (Akt/PI3K/GSK-3β) induced by MPP(+) was partially or totally rectified by chrysotoxine. The results indicated that ROS inhibition, mitochondria protection, NF-κB modulation and regulation of multiple signals determining cell survival and cell death were involved in the protective effects of chrysotoxine against MPP(+) toxicity in SH-SY5Y cells. Given the different toxic profiles of 6-OHDA and MPP(+) as compared to rotenone, our results also indicated that DAT inhibition may partially account for the neuroprotective effects of chrysotoxine. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Mechanisms of CDDO-imidazolide-mediated cytoprotection against acrolein-induced neurocytotoxicity in SH-SY5Y cells and primary human astrocytes.

Science.gov (United States)

Speen, Adam; Jones, Colton; Patel, Ruby; Shah, Halley; Nallasamy, Palanisamy; Brooke, Elizabeth A S; Zhu, Hong; Li, Y Robert; Jia, Zhenquan

2015-10-01

Acrolein is a ubiquitous unsaturated aldehyde has been implicated in the pathogenesis of various neurological disorders. However, limited study has been conducted into potential therapeutic protection and underlying mechanism against acrolein-induced cytotoxicity via upregulation of cellular aldehyde-detoxification defenses. In this study we have utilized RA-differentiated human SH-SY5Y cells and primary human astrocytes to investigate the induction of glutathione (GSH) by the synthetic triterpenoid 2-cyano-3,12-dixooleana-1,9-dien-28-imidazolide (CDDO-Im) and the protective effects CDDO-Im-mediated antioxidant defenses on acrolein toxicity. Acrolein exposure to RA-differentiated SH-SY5Y cells resulted in a significant time dependent depletion of cellular GSH preceding a reduction in cell viability and LDH release. Further, we demonstrated the predominance of cellular GSH in protection against acrolein-induced cytotoxicity. Buthionine sulfoximine (BSO) at 25μM dramatically depleted GSH and significantly potentiated acrolein-induced cytotoxicity. Pretreatment of the cells with 100nM CDDO-Im afforded a dramatic protection against acrolein-induced cytotoxicity. Pretreatment of BSO and CDDO was found to prevent the CDDO-Im-mediated GSH induction and partially reversed the cytoprotective effects of CDDO-Im against acrolein cytotoxicity. Overall, this study represents for the first time the CDDO-Im mediated upregulation of GSH is a predominant mechanism against acrolein-induced neurotoxicity. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells.

Science.gov (United States)

Ramkumar, Muthu; Rajasankar, Srinivasagam; Gobi, Veerappan Venkatesh; Dhanalakshmi, Chinnasamy; Manivasagam, Thamilarasan; Justin Thenmozhi, Arokiasamy; Essa, Musthafa Mohamed; Kalandar, Ameer; Chidambaram, Ranganathan

2017-04-18

Mitochondrial dysfunction and oxidative stress are the main toxic events leading to dopaminergic neuronal death in Parkinson's disease (PD) and identified as vital objective for therapeutic intercession. This study investigated the neuro-protective effects of the demethoxycurcumin (DMC), a derivative of curcumin against rotenone induced neurotoxicity. SH-SY5Y neuroblastoma cells are divided into four experimental groups: untreated cells, cells incubated with rotenone (100 nM), cells treated with DMC (50 nM) + rotenone (100 nM) and DMC alone treated. 24 h after treatment with rotenone and 28 h after treatment with DMC, cell viability was assessed using the MTT assay, and levels of ROS and MMP, plus expression of apoptotic protein were analysed. Rotenone induced cell death in SH-SY5Y cells was significantly reduced by DMC pretreatment in a dose-dependent manner, indicating the potent neuroprotective effects of DMC. Rotenone treatment significantly increases the levels of ROS, loss of MMP, release of Cyt-c and expression of pro-apoptotic markers and decreases the expression of anti-apoptotic markers. Even though the results of the present study indicated that the DMC may serve as a potent therapeutic agent particularly for the treatment of neurodegenerative diseases like PD, further pre-clinical and clinical studies are required.

Alzheimer's disease presenilin-1 exon 9 deletion and L250S mutations sensitize SH-SY5Y neuroblastoma cells to hyperosmotic stress-induced apoptosis

DEFF Research Database (Denmark)

Tanii, H; Ankarcrona, M; Flood, F

2000-01-01

. In the present study, we determined whether PS1 mutations also sensitize cells to hyperosmotic stress-induced apoptosis. For this, we established SH-SY5Y neuroblastoma cell lines stably transfected with wild-type PS1 or either the PS1 exon 9 deletion (deltaE9) or PS1 L250S mutants. Cultured cells were exposed...

Employment and First Year Experience of Beginner Primary School Teachers at Private Educational Institutions

Directory of Open Access Journals (Sweden)

Turgay ÖNTAŞ

2016-10-01

Full Text Available In this study to demonstrate primary school teachers' employment process and their first year teaching in private educational organizations is aimed. The major employer of primary school teachers is public schools that are funded by state. There will be a gap between grade inflation and employment rate when all of the teacher candidates claim to be appointed to public schools. Hence private educational organizations provide alternative career opportunities for teacher candidates. Qualitative methods will be used in this study to display real evidence from the eyes of teachers and also principals. The data have been obtained through documents and interview techniques. After the data have been collected, descriptive analysis has been used. The findings of the study were given within the frame of themes and codes which were determined based on the problem statement. The first theme, career was presented in the context of choosing sector, recruitment and selecting process, professional development, leave the profession. The second theme, factors affecting first years' success was explained in the context of teaching factors, consistency and skills focus. The third theme was presented in a category of pressure, workload, complaining psychological and physical exhaustion, flexible working conditions.

ART restorations and sealants placed in Chinese school children--results after three years.

Science.gov (United States)

Holmgren, C J; Lo, E C; Hu, D; Wan, H

2000-08-01

These were to assess whether ART restorations and sealants could be provided to children in a school environment in China, to assess patient acceptability of the ART approach, and to evaluate on a longitudinal basis the treatments performed. This study was conducted in Deyang, Sichuan Province, in western China. A total of 294 ART restorations were placed in 197 children and 191 fissure sealants were placed in 140 children by five middle-level dentists in four secondary schools. Standard instruments and procedures for ART were used. The restorative material used was a high-strength glass-ionomer (Ketac-Molar, ESPE). The treatments were evaluated annually after placement by the same examiner who had not been involved in the placement of the restorations nor sealants using explorers and mouth-mirrors. At the 3-year examination an independent external examiner evaluated the restorations using USPHS criteria. Most of the children did not report discomfort during treatment and 92% were willing to receive ART restorations again. The cumulative 1-year and 3-year survival rates of small Class I restorations were 99% and 92% respectively. The corresponding figures for large Class I restorations were 90% and 77%. After 3 years, 72% of the sealants were either partially or completely retained. Only 2% of the sealed teeth developed fissure caries and these involved teeth where the sealants had been lost. Similar success rates were found using USPHS criteria. The ART approach for preventing and treating tooth decay in Chinese school children was shown to be appropriate, effective and acceptable. The 3-year survival rates of the restorations were high but were related to the size and type of the restoration.

The Incorporation of the School Age Group of 16-18 Years Old in Gambling

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MSc. Alfred Nela

2015-06-01

Full Text Available The scientific research of this paper focuses on the involvement of the school age group 16-18 years old in sports betting games impacted by beliefs on luck and social groups. For the collection of data literature is utilized, questionnaires are distributed and also focus groups were conducted. The assumptions of the assignment are: a The involvement of school age group 16-18 years in sports betting games are manners learned from their comrades, b The school age group 16-18 years old attribute to fate the participation in sports betting games. Over 64% of responses manifest that they have learned the rules from their companions. The revelations and questionnaire responses argue that over 45% of students in their classes play sports bets, and 34% think that the phenomena of sports betting depends on luck. The major part of this age group do not practice and believe in fortunate rituals or activities. The acquired data reflect that the extent of involvement of this age group is in substantial dimensions. We suggest that the decision-making bodies and service providers should develop strategies in order to reduce the phenomenon.

Mitochondrial Effects of PGC-1alpha Silencing in MPP+ Treated Human SH-SY5Y Neuroblastoma Cells

Directory of Open Access Journals (Sweden)

Qinyong Ye

2017-05-01

Full Text Available The dopaminergic neuron degeneration and loss that occurs in Parkinson’s disease (PD has been tightly linked to mitochondrial dysfunction. Although the aged-related cause of the mitochondrial defect observed in PD patients remains unclear, nuclear genes are of potential importance to mitochondrial function. Human peroxisome proliferator-activated receptor γ coactivator-1alpha (PGC-1α is a multi-functional transcription factor that tightly regulates mitochondrial biogenesis and oxidative capacity. The goal of the present study was to explore the potential pathogenic effects of interference by the PGC-1α gene on N-methyl-4-phenylpyridinium ion (MPP+-induced SH-SY5Y cells. We utilized RNA interference (RNAi technology to probe the pathogenic consequences of inhibiting PGC-1α in the SH-SY5Y cell line. Remarkably, a reduction in PGC-1α resulted in the reduction of mitochondrial membrane potential, intracellular ATP content and intracellular H2O2 generation, leading to the translocation of cytochrome c (cyt c to the cytoplasm in the MPP+-induced PD cell model. The expression of related proteins in the signaling pathway (e.g., estrogen-related receptor α (ERRα, nuclear respiratory factor 1 (NRF-1, NRF-2 and Peroxisome proliferator-activated receptor γ (PPARγ also decreased. Our finding indicates that small interfering RNA (siRNA interference targeting the PGC-1α gene could inhibit the function of mitochondria in several capacities and that the PGC-1α gene may modulate mitochondrial function by regulating the expression of ERRα, NRF-1, NRF-2 and PPARγ. Thus, PGC-1α can be considered a potential therapeutic target for PD.

Report on weather observation at the MONJU website in the 2002 fiscal year

International Nuclear Information System (INIS)

2004-03-01

The weather observation was carried out at two sites (CT and MS-1) by the MONJU website in Tsuruga city, Japan. The observation items are wind direction, wind velocity, temperature and atmospheric stability. The observation results are shown by the average wind velocity (year and month), wind rose in year, appearance frequency of wind direction and velocity, appearance and cumulative frequency of each step of wind velocity, appearance frequency of wind direction, average wind velocity, change of monthly average temperature, appearance frequency of atmospheric stability and observation site. (S.Y.)

Neuroprotective effects of metabotropic glutamate receptor group II and III activators against MPP(+)-induced cell death in human neuroblastoma SH-SY5Y cells: the impact of cell differentiation state.

Science.gov (United States)

Jantas, D; Greda, A; Golda, S; Korostynski, M; Grygier, B; Roman, A; Pilc, A; Lason, W

2014-08-01

Recent studies have documented that metabotropic glutamate receptors from group II and III (mGluR II/III) are a potential target in the symptomatic treatment of Parkinson's disease (PD), however, the neuroprotective effects of particular mGluR II/III subtypes in relation to PD pathology are recognized only partially. In the present study, we investigated the effect of various mGluR II/III activators in the in vitro model of PD using human neuroblastoma SH-SY5Y cell line and mitochondrial neurotoxin MPP(+). We demonstrated that all tested mGluR ligands: mGluR II agonist - LY354740, mGluR III agonist - ACPT-I, mGluR4 PAM - VU0361737, mGluR8 agonist - (S)-3,4-DCPG, mGluR8 PAM - AZ12216052 and mGluR7 allosteric agonist - AMN082 were protective against MPP(+)-evoked cell damage in undifferentiated (UN-) SH-SY5Y cells with the highest neuroprotection mediated by mGluR8-specific agents. However, in retinoic acid- differentiated (RA-) SH-SY5Y cells we found protection mediated only by mGluR8 activators. We also demonstrated the cell proliferation stimulating effect for mGluR4 and mGluR8 PAMs. Next, we showed that the protection mediated by mGluR II/III activators in UN-SH-SY5Y was not accompanied by the modulation of caspase-3 activity, however, a decrease in the number of apoptotic nuclei was found. Finally, we showed that the inhibitor of necroptosis, necrostatin-1 blocked the mGluR III-mediated protection. Altogether our comparative in vitro data add a further proof to neuroprotective effects of mGluR agonists or PAMs and point to mGluR8 as a promising target for neuroprotective interventions in PD. The results also suggest the participation of necroptosis-related molecular pathways in neuroprotective effects of mGluR III activation. Copyright © 2014 Elsevier Ltd. All rights reserved.

Childhood obesity: the extent of the problem among 6-year-old Irish national school children.

Science.gov (United States)

Evans, D S; Glacken, M; Goggin, D

2011-05-01

Childhood obesity is rapidly increasing worldwide. In Ireland, the number of overweight children has trebled over the last decade. The study aimed to provide an assessment of the prevalence of obesity of 6-year-old children in one region of Ireland. Following training, School Public Health Nurses included the measurement of height and weight as part of the annual 'senior infants' school health check for 5453 6-year-old children in 189 schools between 2004 and 2007. Body mass index (BMI) was calculated using the International Obesity Taskforce cut-off points using lmsGrowth (a Microsoft Excel add-in), which uses a child's exact age. Kendall's Tau b was used to determine the reliability of measurements. Prevalence trends were tested using multinomial logistic regression. Pearson's chi-squared test was utilized to assess the statistical significance of differences in BMI by gender, school year, and to compare with similar other Irish studies. Out of the 5453 children measured, 3493 were aged 6 years old. A further 11 were excluded because of incomplete data. Data were analysed for 3482 6-year-old children. Overall, 27% of 6-year-olds were classified as either overweight or obese. A significantly greater proportion of girls are overweight or obese compared with boys (31% compared with 23%). Gender differences have remained relatively stable from 2004-2007. Overall, there have been no significant changes in the level of obesity from 2004-2007. In addition, when comparing with other Irish studies that collected data for 2001/2, there are no significant differences in obesity levels. There is a clear need to urgently prioritize the effective management of obesity. Resourses should now be targeted towards ensuring government policies in Ireland and elsewhere are implemented. © 2010 Blackwell Publishing Ltd.

Finnish Parental Involvement Ethos, Health Support, Health Education Knowledge and Participation: Results from a 2-Year School Health Intervention

Science.gov (United States)

Sormunen, Marjorita; Tossavainen, Kerttu; Turunen, Hannele

2013-01-01

A 2-year, participatory action research school health study focused on developing components for home-school partnerships to support children's health learning process. Two intervention schools implemented strengthened health and collaboration-orientated activities; two control schools followed the national core curriculum without extracurricular…

Sign me up! Determining motivation for high school chemistry students enrolling in a second year chemistry course

Science.gov (United States)

Camarena, Nilda N.

A sample of 108 Pre-AP Chemistry students in Texas participated in a study to determine motivational factors for enrolling in AP Chemistry and University Chemistry. The factors measured were academic attitude, perceptions of chemistry, confidence level in chemistry, and expectations/experiences in the chemistry class. Students completed two questionnaires, one at the beginning of the year and one at the end. Four high school campuses from two school districts in Texas participated. Two campuses were traditional high schools and two were smaller magnet schools. The results from this study are able to confirm that there are definite correlations between academic attitudes, perceptions, confidence level, and experiences and a student's plans to enroll in AP and University Chemistry. The type of school as well as the student's gender seemed to have an influence on a student's plan to enroll in a second year of chemistry.

Psychological stress and health in undergraduate dental students: fifth year outcomes compared with first year baseline results from five European dental schools

NARCIS (Netherlands)

Gorter, R.C.; Freeman, R.; Hammen, S.; Murtomaa, H.; Blinkhorn, A.; Humphris, G.

2008-01-01

Objective: To compare the levels of a series of health-related indicators from a cohort of fifth year dental students from five European schools with their first year scores, and to investigate the relationship between these follow-up measures. Methods: Burnout was measured using the Maslach Burnout

A Summer at the University: A twenty five years experience with High School Students

Science.gov (United States)

Zamorano, Nelson

2014-03-01

After running a summer school for enthusiastic high school students for 25 years, we reached the point where three of my colleagues at the physics department, are exstudents from two physics courses offered (more than ten years ago) within our program. There are also graduates in some others Faculties in different universities. Here we would like to describe the evolution of this project since its beginning, with 60 students in an introductory physics class to the 3000 now attending (January 2014) the around 60 courses offered in almost all areas of knowledge, from theater to Biotechnology. Lately, as we became aware of the relevance of teaching sciences to young kids in elementary school, we started a winter section addressing this group of students. The courses are mainly a hands on experience. In this talk we will comment about our learning experience working on this kind of projects and our projections for the future. Partial travel support from Escuela de Verano.

Parenting behavior at 2 years predicts school-age performance at 7 years in very preterm children.

Science.gov (United States)

Treyvaud, Karli; Doyle, Lex W; Lee, Katherine J; Ure, Alexandra; Inder, Terrie E; Hunt, Rod W; Anderson, Peter J

2016-07-01

Parenting influences child development, but it is unclear whether early parenting behavior can influence school-age outcomes in very preterm (VPT) children, and/or if certain groups of VPT children may be more affected by early parenting behavior. These research questions were examined. Participants were 147 children born parent-child interaction was assessed, and at 7 years CA, general intelligence (IQ), language, executive function, academic skills, and social-emotional functioning were assessed. Higher levels of parent-child synchrony, and parent facilitation, sensitivity and positive affect at 2 years were associated with better child outcomes at 7 years, while higher levels of intrusiveness and negative affect were associated with poorer outcomes. Many of these relationships remained after controlling for early child cognitive development. Interactions between child medical risk (higher/lower) and parenting were limited to child reading, math, and executive functioning outcomes, with stronger relationships for lower medical risk children. The contribution of early parenting to VPT children's school-age performance is significant, with stronger effects for lower medical risk children in some outcomes. These findings support the premise that parenting strategies should be included in the NICU and early interventions programs for VPT infants. © 2015 Association for Child and Adolescent Mental Health.

Remedial Investigation work plan for Bear Creek Valley Operable Unit 2 (Rust Spoil Area, SY-200 Yard, Spoil Area 1) at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

International Nuclear Information System (INIS)

1992-09-01

This Remedial Investigation (RI) work plan contains summaries of geographical, historical, operational, geological, and hydrological information specific to the unit. The potential for release of contamination to receptors through the various media is addressed, and a sampling and analysis plan is presented to determine the extent of release of contamination to the surrounding environment. Proposed activities include walkover radiation surveys at all sites, soil borings at SY-200, piezometer installation and water table sampling at SA-1 and SY-200, and surface water and sediment runoff sampling at all three sites. Data from the site characterization activities will be combined with data from ongoing site-wide monitoring programs (i.e., groundwater, surface water, and biological monitoring) to provide input for a screening-level risk assessment and evaluation of altemative remedial actions

"PCI Reading Program": The Final Report of a Three Year Experimental Study in Brevard Public Schools and Miami-Dade County Public Schools. Research Report

Science.gov (United States)

Toby, Megan; Jaciw, Andrew; Ma, Boya; Lipton, Akiko

2011-01-01

PCI Education conducted a three-year longitudinal study to determine the comparative effectiveness of the "PCI Reading Program" ("PCI") for students with severe disabilities as implemented in Florida's Brevard Public Schools and Miami-Dade County Public Schools. The primary question addressed by the study is whether students…

Public School Teacher Autonomy in the Classroom across School Years 2003-04, 2007-08, and 2011-12. Stats in Brief. NCES 2015-089

Science.gov (United States)

Sparks, Dinah; Malkus, Nat

2015-01-01

This Statistics in Brief explores teacher autonomy in the classroom during the 2003-04, 2007-08, and 2011-12 school years. Using data from the National Center for Education Statistics (NCES) Schools and Staffing Survey (SASS), the Statistics in Brief examines a construct of teacher autonomy based on teachers' responses to six questions regarding…

Effect of Middle School Interventions on Alcohol Misuse and Abuse in Mexican American High School Adolescents: Five-Year Follow-up of a Randomized Clinical Trial.

Science.gov (United States)

Gonzales, Nancy A; Jensen, Michaeline; Tein, Jenn Yun; Wong, Jessie J; Dumka, Larry E; Mauricio, Anne Marie

2018-03-21

Substance abuse preventive interventions frequently target middle school students and demonstrate efficacy to prevent early onset and use of alcohol and illicit drugs. However, evidence of sustained results to prevent later patterns of alcohol misuse and more serious alcohol abuse disorders has been lacking, particularly for US Latino populations. To test whether a universal middle school prevention program can reduce the frequency of alcohol misuse and rates of alcohol use disorder 5 years after implementation with a Mexican American sample. A previous randomized clinical trial was conducted with 516 Mexican American 7th graders and at least 1 parent who identified as having Mexican origin. Three annual cohorts of families were recruited from rosters of 4 middle schools and randomized to the 9-session Bridges/Puentes family-focused group intervention or a workshop control condition. Recruitment, screening, pretest, and randomization occurred in the same academic year for each cohort: 2003-2004, 2004-2005, and 2005-2006. Data acquisition for the follow-up assessments of late-adolescent alcohol misuse and abuse, which were not included in the initial randomized clinical trial, was conducted from September 2009 to September 2014; analysis was conducted between August 2016 and July 2017. In this assessment, 420 children (81.4%) of the sample were included, when the majority were in their final year of high school. The 9-session Bridges/Puentes intervention integrated youth, parent, and family intervention sessions that were delivered in the spring semester at each school, with separate groups for English-dominant vs Spanish-dominant families. The control workshop was offered during the same semester at each school, also in English and Spanish. Primary outcomes were diagnostic assessment of lifetime alcohol use disorder in the 12th grade, 5 years after the intervention, based on the Diagnostic Interview Schedule for Children and past-year frequency of alcohol use

Occupational accidents in dental school: a 10-year retrospective

Directory of Open Access Journals (Sweden)

Viviane Maia Araújo

2009-12-01

Full Text Available Objective: To investigate occupational accidents that occurred during the first 10 years of Fortaleza University (UNIFOR Dental School. Methods: A documental study based on secondary data from the Notification Center of Occupational Accidents of UNIFOR Dental School, reported in the last 10 years. The variables included characteristics of the accidents and of the injured, besides the type of instrument and the resulting injury. Results: Were recorded 160 occupational accidents divided by location, function of the injured and type of accident. It was observed that 63.1% of cases occurred in the Multidisciplinary Clinic, 23.1% in the Integrated Clinic, 8.8% in the laboratories, 1.9% at home, 1.25% in the Surgical Center, 1.25% in the Material Sterilization Central and 0.6% during the training outside university. Concerning the injured, 90.6% of the victims were undergraduates, 5.0% staff, 3.8% teachers and 0.6% patients. Regarding the injury, 40.6% were penetrating bloody injuries, 11.9% cutting bloody injuries, 2.5% cutting non-bloody injuries, 5% burns, 5% penetrating bloody injuries/cutting bloody injuries, 2.5% were injuries with maceration, 1.9% injuries causing ocular trauma and 0.6% of an incident of dog bite. Conclusion: It was concluded that penetrating injuries were prevalent and these occurred mostly in the multidisciplinary clinic, where students of earlier periods of dental course work, indicating that the experience in dental practice generates more care with safety.

School-Sponsored Before, After and Extended School Year Programs: The Role of the School Nurse. Position Statement

Science.gov (United States)

Clark, Elizabeth; Buswell, Sue Ann; Morgitan, Judith; Compton, Linda; Westendorf, Georgene; Chau, Elizabeth

2014-01-01

It is the position of the National Association of School Nurses (NASN) that the registered professional school nurse (hereinafter referred to as school nurse) has the educational and clinical background to coordinate the necessary school health services to provide students with the same health, nutrition, and safety needs while attending…

Documentation to the NCES Common Core of Data Local Education Agency Universe Survey: School Year 2011-12. Provisional Version 1a. NCES 2014-035

Science.gov (United States)

Keaton, Patrick

2014-01-01

This documentation is for the provisional version 1a file of the National Center for Education Statistics' (NCES) Common Core of Data (CCD) Local Education Agency (LEA) Universe Survey for SY 2011-12. It contains a brief description of the data collection, along with information required to understand and access the data file. The CCD is a…

The Effects of Year-Round Schools on the Hospitality Industry's Seasonal Labor Force in the State of Tennessee.

Science.gov (United States)

Pickeral, Lyn M.; Hubbard, Susan

2002-01-01

Data collected from 66 managers in Tennessee tourist attractions indicate that 53 percent of seasonal workers in Tennessee come from the school system. The proposal to implement year-round schools would drastically increase the tourism industry's labor shortage. An alternative labor force needs to be identified and the issue of year-round schools…

Numerical simulation of Hanford Tank 241-SY-101 jet initiated fluid dynamics

International Nuclear Information System (INIS)

Trent, D.S.; Michener, T.E.

1994-01-01

The episodic Gas Release Events (GREs) that have characterized the behavior of Hanford tank 241-SY-101 for the past several years are thought to result from the entrapment of gases generated in the settled solids, i.e., sludge, layer of the tank. Gases consisting of about 36% hydrogen by volume, which are generated by complicated and poorly understood radiological and chemical processes, are apparently trapped in the settled solids layer until their accumulation initiates a buoyant upset of this layer, abruptly releasing large quantities of gas. Once concept for preventing the gas accumulation is to mobilize the settled materials with jet mixing. It is suggested that continual agitation of the settled solids using a mixer pump would free the gas bubbles so that they could continually escape, thus mitigating the potential for accumulation of flammable concentrations of hydrogen in the tank dome space following a GRE. A pump test is planned to evaluate the effectiveness of the jet mixing mitigation concept. The pump will circulate liquid from the upper layer of the tank, discharging it through two horizontal jets located approximately 2 1/2 ft above the tank floor. To prepare for start-up of this pump test, technical, operation, and safety questions concerning an anticipated gas release were addressed by numerical simulation using the TEMPEST computer code. Simulations of the pump initiated gas release revealed that the amount of gas that could potentially be released to the tank dome space is very sensitive to the initial conditions assumed for the amount and distribution of gas in the sludge layer. Calculations revealed that within the assumptions regarding gas distribution and content, the pump might initiate a rollover--followed by a significant gas release--if the sludge layer contains more than about 13 to 14% gas distributed with constant volume fraction

Long-term impact of a chef on school lunch consumption: findings from a 2-year pilot study in Boston middle schools.

Science.gov (United States)

Cohen, Juliana F W; Smit, Liesbeth A; Parker, Ellen; Austin, S Bryn; Frazier, A Lindsay; Economos, Christina D; Rimm, Eric B

2012-06-01

School cafeterias can play an important role in providing healthy meals. Although schools participating in the National School Lunch Program are required to meet minimum program standards, advocates recommend that innovations be sought to enhance menu dietary quality. This study evaluated the Chef Initiative, a 2-year pilot study in two Boston middle schools, designed to increase the availability and consumption of healthier school foods. Between 2007 and 2009, a professional chef trained cafeteria staff to prepare healthier school lunches (ie, more whole grains, fresh/frozen fruits and vegetables, and less sugar, salt, saturated fats, and trans fats). Meal nutrient compositions were monitored from 2007 to 2009, and a plate waste study conducted in the spring of 2009 compared food selection and consumption patterns among students at Chef Initiative schools, with students receiving standard school lunches at two matched control schools. Paired t tests and descriptive statistics were used to examine differences in menus and mixed-model analysis of variance was used to analyze differences in students' food selection and consumption between Chef Initiative and control schools. Overall, the Chef Initiative schools provided healthier lunches and the percent of foods consumed at Chef Initiative and control schools were similar (61.6% vs 57.3%; P=0.63). Of the areas targeted, there was greater whole-grain selection and vegetable consumption; 51% more students selected whole grains (P=0.02) and students consumed 0.36 more vegetable servings/day (P=0.01) at Chef Initiative schools. The potential of chefs collaborating with cafeteria staff to improve the availability, selection, and consumption of healthier meals is promising. Copyright © 2012 Academy of Nutrition and Dietetics. Published by Elsevier Inc. All rights reserved.

Effects of School Quality, School Citizenship Policy, and Student Body Composition on the Acquisition of Citizenship Competences in the Final Year of Primary Education

Science.gov (United States)

Dijkstra, Anne Bert; Geijsel, Femke; Ledoux, Guuske; van der Veen, Ineke; ten Dam, Geert

2015-01-01

This study examines the effects of general educational quality of schools, school citizenship policy, and ethnic homogeneity of the student body on the acquisition of citizenship competences in the final year of primary education. The theoretical framework is based on developmental, psychological, and sociological studies into effects of social…

Neuroprotective effects of glyceryl nonivamide against microglia-like cells and 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y human dopaminergic neuroblastoma cells.

Science.gov (United States)

Lin, Yi-Chin; Uang, Hao-Wei; Lin, Rong-Jyh; Chen, Ing-Jun; Lo, Yi-Ching

2007-12-01

Glyceryl nonivamide (GLNVA), a vanilloid receptor (VR) agonist, has been reported to have calcitonin gene-related peptide-associated vasodilatation and to prevent subarachnoid hemorrhage-induced cerebral vasospasm. In this study, we investigated the neuroprotective effects of GLNVA on activated microglia-like cell mediated- and proparkinsonian neurotoxin 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in human dopaminergic neuroblastoma SH-SY5Y cells. In coculture conditions, we used lipopolysaccharide (LPS)-stimulated BV-2 cells as a model of activated microglia. LPS-induced neuronal death was significantly inhibited by diphenylene iodonium (DPI), an inhibitor of NADPH oxidase. However, capsazepine, the selective VR1 antagonist, did not block the neuroprotective effects of GLNVA. GLNVA reduced LPS-activated microglia-mediated neuronal death, but it lacked protection in DPI-pretreated cultures. GLNVA also decreased LPS activated microglia induced overexpression of neuronal nitric-oxide synthase (nNOS) and glycoprotein 91 phagocyte oxidase (gp91(phox)) on SH-SY5Y cells. Pretreatment of BV-2 cells with GLNVA diminished LPS-induced nitric oxide production, overexpression of inducible nitric-oxide synthase (iNOS), and gp91(phox) and intracellular reactive oxygen species (iROS). GLNVA also reduced cyclooxygenase (COX)-2 expression, inhibitor of nuclear factor (NF)-kappaB (IkappaB)alpha/IkappaBbeta degradation, NF-kappaB activation, and the overproduction of tumor necrosis factor-alpha, interleukin (IL)-1beta, and prostaglandin E2 in BV-2 cells. However, GLNVA augmented anti-inflammatory cytokine IL-10 production on LPS-stimulated BV-2 cells. Furthermore, in 6-OHDA-treated SH-SY5Y cells, GLNVA rescued the changes in condensed nuclear and apoptotic bodies, prevented the decrease in mitochondrial membrane potential, and reduced cells death. GLNVA also suppressed accumulation of iROS and up-regulated heme oxygenase-1 expression. 6-OHDA-induced overexpression of nNOS, i

Pretreatment of MQA, a caffeoylquinic acid derivative compound, protects against H2O2-induced oxidative stress in SH-SY5Y cells.

Science.gov (United States)

Tian, Xing; Gao, Lingyue; An, Li; Jiang, Xiaowen; Bai, Junpeng; Huang, Jian; Meng, Weihong; Zhao, Qingchun

2016-12-01

Compound MQA (1,5-O-dicaffeoyl-3-O-[4-malic acid methyl ester]-quinic acid) is a natural caffeoylquinic acid derivative isolated from Arctium lappa L. roots. This study aims to explore the neuroprotective effects of MQA against hydrogen peroxide (H 2 O 2 )-induced oxidative stress in SH-SY5Y neuroblastoma cells. The SH-SY5Y cells were divided into four groups, including control, 20 μM MQA, 200 μM H2O2, 200 μM H2O2 + 20 μM MQA groups. The effects of MQA on H 2 O 2 -induced cell death were measured by MTT and LDH assays. Hoechst 33342 and Annexin V-PI double staining were used to observed H2O2-induced apoptosis. Also, the effects of MQA on antioxidant system and mitochondrial pathway were explored. Further, steady-state phosphorylation levels of ERK1/2, Akt and GSK-3β were examined by Western blot analysis. Pretreatment with MQA prevented cell death in SH-SY5Y cells exposed to 200 μM H2O2 for 3 h. Meanwhile, Hoechst 33342 and Annexin V-PI double staining showed that MQA attenuated H 2 O 2 -induced apoptosis. These changes are related to elevation in SOD activity, reduction in MDA production and ROS formation, and increases in mitochondrial membrane potential (MMP). In addition, the potential mechanisms of MQA against H 2 O 2 -induced apoptosis are associated with increases in the Bcl-2/Bax ratio, decreases in cytochrome c release, caspase-3 and caspase-9 expressions, phosphorylation of ERK1/2, and dephosphorylation of AKT and GSK-3β. These findings suggest that protective effects of MQA against H 2 O 2 -induced apoptosis might be associated with mitochondrial apoptosis, ERK1/2 and AKT/GSK-3β pathway.

The biologic role of ganglioside in neuronal differentiation--effects of GM1 ganglioside on human neuroblastoma SH-SY5Y cells.

OpenAIRE

Lee, M. C.; Lee, W. S.; Park, C. S.; Juhng, S. W.

1994-01-01

Human neuroblastoma SH-SY5Y cell is a cloned cell line which has many attractive features for the study of neuronal proliferation and neurite outgrowth, because it has receptors for insulin, IGF-I and PDGF. Gangliosides are sialic acid containing glycosphingolipids which form an integral part of the plasma membrane of many mammalian cells. They inhibit cell growth mediated by tyrosine kinase receptors and ligand-stimulated tyrosine kinase activity, and autophosphorylation of EGF(epidermal gro...

Safety assessment for proposed pump mixing operations to mitigate episodic gas releases in tank 241-101-SY: Hanford Site, Richland, Washington

Energy Technology Data Exchange (ETDEWEB)

Lentsch, J.W., Westinghouse Hanford

1996-05-16

This safety assessment addresses each of the elements required for the proposed action to remove a slurry distributor and to install, operate, and remove a mixing pump in Tank 241-SY-101, which is located within the Hanford Site, Richland, Washington. The proposed action is required as part of an ongoing evaluation of various mitigation concepts developed to eliminate episodic gas releases that result in hydrogen concentrations in the tank dome space that exceed the lower flammability limit.

The relationship between school lunch attendance and the food intakes of French schoolchildren aged 3-17 years.

Science.gov (United States)

Dubuisson, Carine; Lioret, Sandrine; Dufour, Ariane; Calamassi-Tran, Gloria; Volatier, Jean-Luc; Lafay, Lionel; Turck, Dominique

2015-06-01

Recently, school meal composition regulations have been implemented in France in order to improve the nutritional status of children. The present study investigated the link between school lunch attendance and the food intakes of schoolchildren aged 3-17 years. Second French cross-sectional dietary survey (2006-2007). Eating frequencies were assessed for twenty-four food groups with a 7 d food record. Eating locations were recorded for main meals. Food group intakes at weekday lunches were compared for the school canteen and for other locations. The children's overall dietary intake was compared based on school lunch attendance. Mainland France. Schoolchildren aged 3-17 years (n 1068). Lunchtime food intake differed between the school canteen and other locations. Some intakes at school canteens were more in accordance with the regulations (more fruit and vegetables, fish and dairy products, and less sandwiches, soft drinks, chocolate and confectionery), whereas others highlighted needs for improvement (more sweet biscuits and pastries, ice cream and dairy desserts, pizzas and salty pastries). Many of these differences were also observed in the children's overall diet: children regularly attending school lunches ate more mashed fruit, fish and sweet biscuits or pastries, and less sandwiches and soft drinks. The link between school lunch attendance and overall diet was less pronounced in secondary-school children. School canteen attendance is associated with both potentially beneficial and deleterious differences in the lunchtime and overall diets of French children. These findings are important to consider when setting national regulations for school meal composition.

Glass ionomer ART sealants in Chinese school children-6-year results.

Science.gov (United States)

Holmgren, Christopher J; Lo, Edward C M; Hu, Deyu

2013-09-01

To evaluate longitudinally ART sealants placed in Chinese school children under field conditions. 191 ART sealants were placed in 140 children, aged 11-14 years, by five assistant dentists in four secondary schools in Deyang, Sichuan Province, China. Teeth selected for sealing were those with pits and fissures that were deep or showing early enamel caries. Teeth were excluded if there was obvious cavitation extending into dentine. Standard instruments and procedures for ART sealants were used. The material used was a high-viscosity glass-ionomer (Ketac-Molar, 3MESPE) that was inserted into the pits and fissures with the "press-finger" technique. The status of the sealants was evaluated annually over 6 years after placement by the same examiner who was not involved in the placement of the sealants using explorers, mouth-mirrors and an intra-oral fibre-optic light. No missing sealants were replaced during the study. 107 sealants (56% of the original) were examined after 6 years. The cumulative survival rates of the sealants (partially or fully retained) after 2, 4 and 6 years were 79%, 68% and 59%, respectively. Caries prevention lagged the fall in sealant survival but remained high throughout the study period, being over 90% in the first 4 years and 85% after 6 years. ART sealants placed under field conditions in Chinese schoolchildren have a high retention rate. Missing sealants should be replaced to maintain their preventive efficacy. The sealing of pits and fissures can be an effective caries preventive approach. Resin-based sealants have the disadvantage in that they require an optimal level of moisture control during placement. In children and in outreach situations glass ionomer ART sealants, which are more moisture tolerant, can offer a viable alternative. Copyright © 2013 Elsevier Ltd. All rights reserved.

Genome-Wide Polygenic Scores Predict Reading Performance Throughout the School Years.

Science.gov (United States)

Selzam, Saskia; Dale, Philip S; Wagner, Richard K; DeFries, John C; Cederlöf, Martin; O'Reilly, Paul F; Krapohl, Eva; Plomin, Robert

2017-07-04

It is now possible to create individual-specific genetic scores, called genome-wide polygenic scores (GPS). We used a GPS for years of education ( EduYears ) to predict reading performance assessed at UK National Curriculum Key Stages 1 (age 7), 2 (age 12) and 3 (age 14) and on reading tests administered at ages 7 and 12 in a UK sample of 5,825 unrelated individuals. EduYears GPS accounts for up to 5% of the variance in reading performance at age 14. GPS predictions remained significant after accounting for general cognitive ability and family socioeconomic status. Reading performance of children in the lowest and highest 12.5% of the EduYears GPS distribution differed by a mean growth in reading ability of approximately two school years. It seems certain that polygenic scores will be used to predict strengths and weaknesses in education.

Anxiety of School-Age Childre (10 – 12 Years) Face Menarche at Mojoroto Village Kediri City

OpenAIRE

Wati, Susi Erna

2015-01-01

Child age at ranging Elementary School 6 to 12 years, this term constitute school term. Child those are on SD's early class is child that lies on early age elongation. Early age term constitute child developing term that short but constitutes term that really necessary for its life. Therefore, on this term all proprietary potency child needs to be pushed so will optimal ala amends. Menarche constitute first menstruating that ordinary happening deep age range 10 to 12 years. All this time a ...

Computer system design description for SY-101 hydrogen mitigation test project data acquisition and control system (DACS-1). Revision 1

International Nuclear Information System (INIS)

Truitt, R.W.

1994-01-01

This document provides descriptions of components and tasks that are involved in the computer system for the data acquisition and control of the mitigation tests conducted on waste tank SY-101 at the Hanford Nuclear Reservation. The system was designed and implemented by Los alamos National Laboratory and supplied to Westinghouse Hanford Company. The computers (both personal computers and specialized data-taking computers) and the software programs of the system will hereafter collectively be referred to as the DACS (Data Acquisition and Control System)

PPARbeta agonists trigger neuronal differentiation in the human neuroblastoma cell line SH-SY5Y.

Science.gov (United States)

Di Loreto, S; D'Angelo, B; D'Amico, M A; Benedetti, E; Cristiano, L; Cinque, B; Cifone, M G; Cerù, M P; Festuccia, C; Cimini, A

2007-06-01

Neuroblastomas are pediatric tumors originating from immature neuroblasts in the developing peripheral nervous system. Differentiation therapies could help lowering the high mortality due to rapid tumor progression to advanced stages. Oleic acid has been demonstrated to promote neuronal differentiation in neuronal cultures. Herein we report on the effects of oleic acid and of a specific synthetic PPARbeta agonist on cell growth, expression of differentiation markers and on parameters responsible for the malignancy such as adhesion, migration, invasiveness, BDNF, and TrkB expression of SH-SY5Y neuroblastoma cells. The results obtained demonstrate that many, but not all, oleic acid effects are mediated by PPARbeta and support a role for PPARbeta in neuronal differentiation strongly pointing towards PPAR ligands as new therapeutic strategies against progression and recurrences of neuroblastoma.

Cardiopulmonary Resuscitation Training in Schools Following 8 Years of Mandating Legislation in Denmark: A Nationwide Survey.

Science.gov (United States)

Malta Hansen, Carolina; Zinckernagel, Line; Ersbøll, Annette Kjær; Tjørnhøj-Thomsen, Tine; Wissenberg, Mads; Lippert, Freddy Knudsen; Weeke, Peter; Gislason, Gunnar Hilmar; Køber, Lars; Torp-Pedersen, Christian; Folke, Fredrik

2017-03-14

School cardiopulmonary resuscitation (CPR) training has become mandatory in many countries, but whether legislation has translated into implementation of CPR training is largely unknown. We assessed CPR training of students following 8 years of legislative mandates in Denmark. A nationwide cross-sectional survey of Danish school leadership (n=1240) and ninth-grade homeroom teachers (n=1381) was carried out for school year 2013-2014. Qualitative interviews and the Theory of Planned Behavior were used to construct the survey. Logistic regression models were employed to identify factors associated with completed CPR training. Information from 63.1% of eligible schools was collected: 49.3% (n=611) of leadership and 48.2% (n=665) of teachers responded. According to teachers, 28.4% (95% CI 25.0% to 32.0%) and 10.3% (95% CI 8.1% to 12.8%) of eligible classes had completed CPR and automated external defibrillator training, respectively. Among leadership, 60.2% (95% CI 56.2% to 64.1%) reported CPR training had occurred during the 3 years prior to the survey. Factors associated with completed CPR training included believing other schools were conducting training (odds ratio [OR] 9.68 [95% CI 4.65-20.1]), awareness of mandating legislation (OR 4.19 [95% CI 2.65-6.62]), presence of a school CPR training coordinator (OR 3.01 [95% CI 1.84-4.92]), teacher feeling competent to conduct training (OR 2.78 [95% CI 1.74-4.45]), and having easy access to training material (OR 2.08 [95% CI 1.57-2.76]). Despite mandating legislation, school CPR training has not been successfully implemented. Completed CPR training was associated with believing other schools were conducting training, awareness of mandating legislation, presence of a school CPR training coordinator, teachers teacher feeling competent to conduct training, and having easy access to training material. Facilitating these factors may increase rates of school CPR training. © 2017 The Authors. Published on behalf of the

[Pt(O,O’-acac)(γ-acac)(DMS)] Alters SH-SY5Y Cell Migration and Invasion by the Inhibition of Na+/H+ Exchanger Isoform 1 Occurring through a PKC-ε/ERK/mTOR Pathway

Science.gov (United States)

Muscella, Antonella; Vetrugno, Carla; Calabriso, Nadia; Cossa, Luca Giulio; De Pascali, Sandra Angelica; Fanizzi, Francesco Paolo; Marsigliante, Santo

2014-01-01

We previously showed that [Pt(O,O’-acac)(γ-acac)(DMS)] ([Pt(acac)2(DMS)]) exerted substantial cytotoxic effects in SH-SY5Y neuroblastoma cells, and decreased metalloproteases (MMPs) production and cells migration in MCF-7 breast cancer cells. The ubiquitously distributed sodium-hydrogen antiporter 1 (NHE1) is involved in motility and invasion of many solid tumours. The present study focuses on the effects of [Pt(acac)2(DMS)] in SH-SY5Y cell migration and also on the possibility that NHE1 may be involved in such effect. After sublethal [Pt(acac)2(DMS)] treatment cell migration was examined by wounding assay and cell invasion by transwell assay. NHE1 activity was measured in BCECF-loaded SH-SY5Y as the rate of Na+-dependent intracellular pH recovery in response to an acute acid pulse. Gelatin zymography for MMP-2/9 activities, Western blottings of MMPs, MAPKs, mTOR, S6 and PKCs and small interfering RNAs to PKC-ε/-δ mRNA were performed. Sublethal concentrations of [Pt(acac)2(DMS)] decreases NHE1 activity, inhibites cell migration and invasion and decreases expression and activity of MMP-2 and -9. [Pt(acac)2(DMS)] administered to SH-SY5Y cells provokes the increment of ROS, generated by NADPH oxidase, responsible for the PKC-ε and PKC-δ activation. Whilst PKC-δ activates p38/MAPK, responsible for the inhibition of MMP-2 and -9 secretion, PKC-ε activates a pathway made of ERK1/2, mTOR and S6K responsible for the inhibition of NHE1 activity and cell migration. In conclusion, we have shown a drastic impairment in tumour cell metastatization in response to inhibition of NHE1 and MMPs activities by [Pt(acac)2(DMS)] occurring through a novel mechanism mediated by PKC-δ/-ε activation. PMID:25372487

[Pt(O,O'-acac)(γ-acac)(DMS)] alters SH-SY5Y cell migration and invasion by the inhibition of Na+/H+ exchanger isoform 1 occurring through a PKC-ε/ERK/mTOR Pathway.

Science.gov (United States)

Muscella, Antonella; Vetrugno, Carla; Calabriso, Nadia; Cossa, Luca Giulio; De Pascali, Sandra Angelica; Fanizzi, Francesco Paolo; Marsigliante, Santo

2014-01-01

We previously showed that [Pt(O,O'-acac)(γ-acac)(DMS)] ([Pt(acac)2(DMS)]) exerted substantial cytotoxic effects in SH-SY5Y neuroblastoma cells, and decreased metalloproteases (MMPs) production and cells migration in MCF-7 breast cancer cells. The ubiquitously distributed sodium-hydrogen antiporter 1 (NHE1) is involved in motility and invasion of many solid tumours. The present study focuses on the effects of [Pt(acac)2(DMS)] in SH-SY5Y cell migration and also on the possibility that NHE1 may be involved in such effect. After sublethal [Pt(acac)2(DMS)] treatment cell migration was examined by wounding assay and cell invasion by transwell assay. NHE1 activity was measured in BCECF-loaded SH-SY5Y as the rate of Na+-dependent intracellular pH recovery in response to an acute acid pulse. Gelatin zymography for MMP-2/9 activities, Western blottings of MMPs, MAPKs, mTOR, S6 and PKCs and small interfering RNAs to PKC-ε/-δ mRNA were performed. Sublethal concentrations of [Pt(acac)2(DMS)] decreases NHE1 activity, inhibits cell migration and invasion and decreases expression and activity of MMP-2 and -9. [Pt(acac)2(DMS)] administered to SH-SY5Y cells provokes the increment of ROS, generated by NADPH oxidase, responsible for the PKC-ε and PKC-δ activation. Whilst PKC-δ activates p38/MAPK, responsible for the inhibition of MMP-2 and -9 secretion, PKC-ε activates a pathway made of ERK1/2, mTOR and S6K responsible for the inhibition of NHE1 activity and cell migration. In conclusion, we have shown a drastic impairment in tumour cell metastatization in response to inhibition of NHE1 and MMPs activities by [Pt(acac)2(DMS)] occurring through a novel mechanism mediated by PKC-δ/-ε activation.

The Ties That Bind: Linkages among Secondary Schools, Two-Year Colleges, and Baccalaureate Institutions.

Science.gov (United States)

Lorence, James J.

This document discusses the roles of secondary schools, two-year colleges, and the upper level university in the University of Wisconsin System. Because of close ties with the host communities, Wisconsin's two-year institutions are uniquely situated to function as community resources. The paper discusses the advantages of a collaborative…

The effects of Nordic school meals on concentration and school performance in 8- to 11-year-old children in the OPUS School Meal Study: a cluster-randomised, controlled, cross-over trial

DEFF Research Database (Denmark)

Sørensen, Louise B.; Dyssegaard, Camilla B.; Damsgaard, Camilla T.

2015-01-01

. The OPUS (Optimal well-being, development and health for Danish children through a healthy New Nordic Diet) School Meal Study was a cluster-randomised, controlled, cross-over trial comparing a healthy school meal programme with the usual packed lunch from home (control) each for 3 months (NCT 01457794......It is widely assumed that nutrition can improve school performance in children; however, evidence remains limited and inconclusive. In the present study, we investigated whether serving healthy school meals influenced concentration and school performance of 8- to 11-year-old Danish children...... than reading speed. There was no effect on overall math performance or outcomes from the LRS. In conclusion, school meals did not affect CP, but improved reading performance, which is a complex cognitive activity that involves inference, and increased errors related to impulsivity and inattention...

Finnish parental involvement ethos, health support, health education knowledge and participation: results from a 2-year school health intervention.

Science.gov (United States)

Sormunen, Marjorita; Tossavainen, Kerttu; Turunen, Hannele

2013-04-01

A 2-year, participatory action research school health study focused on developing components for home-school partnerships to support children's health learning process. Two intervention schools implemented strengthened health and collaboration-orientated activities; two control schools followed the national core curriculum without extracurricular activities. The parents of fourth-grade pupils (10-11 years at baseline) completed questionnaires before intervention in spring 2008 (N = 348) and after intervention in spring 2010 (N = 358). A two-way analysis of variance was conducted to determine whether time (2008/2010) and group (intervention/control) influenced parents' perceptions and experiences of parental involvement, health education and health support received from the school. Compared with controls, the intervention schools' parents experienced greater involvement ethos (Cohen's d = 0.57, P education (Cohen's d = 0.60, P = 0.02) and health support (Cohen's d = 0.35, P = 0.02). Health education participation among parents increased only partially during the intervention (Cohen's d = -0.12, P = 0.193). School health interventions based on schools' needs may have the potential to influence positively the relationship between home and school and increase the visibility of health education. The study was undertaken within the Schools for Health in Europe program.