WorldWideScience

Sample records for satheesh sk torres

  1. Torres Blancas

    Directory of Open Access Journals (Sweden)

    José Manuel López-Peláez

    2016-01-01

    Full Text Available Nella Madrid degli anni sessanta l’architetto navarro Sáenz de Oíza sperimenta la costruzione di un edificio residenziale a torre in cemento armato. Risultato della felice relazione con un imprenditore mecenate, Juan Huarte, l’edificio si articola nella variazione tipologica di alloggi ad L e duplex, di locali per uffici e servizi, fino agli spazi ad uso comune, come la piscina e il ristorante della terrazza sul tetto, per proporsi come modello di piccola città autosufficiente. / In 1960s Madrid, the Navarrese architect Sáenz de Oíza tried constructing a residential tower in reinforced concrete. The happy result of a partnership with an entrepreneur patron, Juan Huarte, the building is split into L-shaped dwellings and duplexes, rooms for offices and services, as well as communal spaces, such as a swimming pool and a restaurant on the roof terrace, to exemplify a model of a small, self-sufficient town.

  2. Grúas-torre

    Directory of Open Access Journals (Sweden)

    Chinchilla, M.

    1964-09-01

    Full Text Available The current large volume of construction, and the new building methods, including large scale préfabrication, have made it necessary to develop adequate machinery to cope with the new requirements. These include the quick distribution of materials and the lifting of large and heavy items. One of these machines which has now attained a widespread use is the tower crane, both of the fixed and moveable type, either on tyres, rails, or of the climbing type. Although these cranes have been known for the last fifty years, their full development only began after the last World War. Their most usual features and varieties make it possible to classify these cranes in a table, which includes most of the models now in standard use.Los nuevos volúmenes de edificación, y los nuevos sistemas, como la prefabricación, han exigido el desarrollo de la maquinaria auxiliar correspondiente, con el fin de poder cubrir las necesidades de alimentación de la obra, reparto de materiales y elevación de cargas y piezas voluminosas. Entre las máquinas auxiliares que h a n obtenido una verdadera mayoría, de edad se encuentran las grúas-torre, fijas, móviles sobre neumáticos o carriles y trepadoras, las cuales, pese a ser conocidas y empleadas desde los años 10, han encontrado su verdadero campo de aplicación después de la segunda guerra mundial. Sus características más comunes respecto a elementos que constituyen la mayor parte de los modelos, permiten agruparlas en una tabla en la que quedan incluidas casi todas las de uso normal.

  3. Skífan

    OpenAIRE

    Elín Ólafsdóttir

    2011-01-01

    Skífan er eina fyrirtækið hér á landi sem sérhæfir sig í sölu á tónlist, mynddiskum, tölvuleikjum og leikjatölvum. Sú atvinnugrein sem Skífan tilheyrir hefur fengið að finna töluvert fyrir þróun internetsins þar sem mögulegt er að hlaða þessu efni niður hvort sem er með löglegum eða ólöglegum hætti. Markmið verkefnisins var að skoða hver áhrif sviðsmynda væru á rekstrarlíkan Skífunnar. Höfundur fór í gegnum atvinnuvega- og samkeppnisgreiningu með aðstoð eiganda og rekstrarstjóra Skífunnar...

  4. A cryptic microbial community persists within micropropagated Bouteloua eriopoda (Torr.) Torr. cultures

    Science.gov (United States)

    Higher plants are ubiquitously colonized with fungal endophytes, which often lack readily detectable structures. This study examines the diverse endophyte population within a single line of micropropagated Bouteloua eriopoda (Torr.) Torr., using microscopy and comparison of internal spacer (ITS) ge...

  5. Sk@m i cyberspace

    DEFF Research Database (Denmark)

    Holm, Claus

    2009-01-01

    Terapi på nettet er en ny mulighed. Men hjælper den klienter med at tale om skambelagte temaer, eller risikerer den at skabe en uansvarlig og skamløs terapisituation? Den norske forfatter, psykiater og psykoterapeut Finn Skårderud vurderer fordele og ulemper ved terapi i cyberspace....

  6. Measurements of the branching fractions for $D^+\\to K^0_SK^0_SK^+$, $K^0_SK^0_S\\pi^+$ and $D^0\\to K^0_SK^0_S$, $K^0_SK^0_SK^0_S$

    CERN Document Server

    Ablikim, M; Ahmed, S; Ai, X C; Albayrak, O; Albrecht, M; Ambrose, D J; Amoroso, A; An, F F; An, Q; Bai, J Z; Ferroli, R Baldini; Ban, Y; Bennett, D W; Bennett, J V; Berger, N; Bertani, M; Bettoni, D; Bian, J M; Bianchi, F; Boger, E; Boyko, I; Briere, R A; Cai, H; Cai, X; Cakir, O; Calcaterra, A; Cao, G F; Cetin, S A; Chai, J; Chang, J F; Chelkov, G; Chen, G; Chen, H S; Chen, J C; Chen, M L; Chen, S; Chen, S J; Chen, X; Chen, X R; Chen, Y B; Cheng, H P; Chu, X K; Cibinetto, G; Dai, H L; Dai, J P; Dbeyssi, A; Dedovich, D; Deng, Z Y; Denig, A; Denysenko, I; Destefanis, M; DeMori, F; Ding, Y; Dong, C; Dong, J; Dong, L Y; Dong, M Y; Dou, Z L; Du, S X; Duan, P F; Fan, J Z; Fang, J; Fang, S S; Fang, X; Fang, Y; Farinelli, R; Fava, L; Fedorov, O; Feldbauer, F; Felici, G; Feng, C Q; Fioravanti, E; Fritsch, M; Fu, C D; Gao, Q; Gao, X L; Gao, Y; Gao, Z; Garzia, I; Goetzen, K; Gong, L; Gong, W X; Gradl, W; Greco, M; Gu, M H; Gu, Y T; Guan, Y H; Guo, A Q; Guo, L B; Guo, R P; Guo, Y; Guo, Y P; Haddadi, Z; Hafner, A; Han, S; Hao, X Q; Harris, F A; He, K L; Heinsius, F H; Held, T; Heng, Y K; Holtmann, T; Hou, Z L; Hu, C; Hu, H M; Hu, J F; Hu, T; Hu, Y; Huang, G S; Huang, J S; Huang, X T; Huang, X Z; Huang, Y; Huang, Z L; Hussain, T; Ji, Q; Ji, Q P; Ji, X B; Ji, X L; Jiang, L W; Jiang, X S; Jiang, X Y; Jiao, J B; Jiao, Z; Jin, D P; Jin, S; Johansson, T; Julin, A; Kalantar-Nayestanaki, N; Kang, X L; Kang, X S; Kavatsyuk, M; Ke, B C; Kiese, P; Kliemt, R; Kloss, B; Kolcu, O B; Kopf, B; Kornicer, M; Kupsc, A; Kuhn, W; Lange, J S; Lara, M; Larin, P; Leithoff, H; Leng, C; Li, C; Li, Cheng; Li, D M; Li, F; Li, F Y; Li, G; Li, H B; Li, H J; Li, J C; Li, Jin; Li, K; Li, K; Li, Lei; Li, P R; Li, Q Y; Li, T; Li, W D; Li, W G; Li, X L; Li, X N; Li, X Q; Li, Y B; Li, Z B; Liang, H; Liang, Y F; Liang, Y T; Liao, G R; Lin, D X; Liu, B; Liu, B J; Liu, C X; Liu, D; Liu, F H; Liu, Fang; Liu, Feng; Liu, H B; Liu, H H; Liu, H H; Liu, H M; Liu, J; Liu, J B; Liu, J P; Liu, J Y; Liu, K; Liu, K Y; Liu, L D; Liu, P L; Liu, Q; Liu, S B; Liu, X; Liu, Y B; Liu, Y Y; Liu, Z A; Liu, Zhiqing; Loehner, H; Lou, X C; Lu, H J; Lu, J G; Lu, Y; Lu, Y P; Luo, C L; Luo, M X; Luo, T; Luo, X L; Lyu, X R; Ma, F C; Ma, H L; Ma, L L; Ma, M M; Ma, Q M; Ma, T; Ma, X N; Ma, X Y; Ma, Y M; Maas, F E; Maggiora, M; Malik, Q A; Mao, Y J; Mao, Z P; Marcello, S; Messchendorp, J G; Mezzadri, G; Min, J; Mitchell, R E; Mo, X H; Mo, Y J; Morales, C Morales; Muchnoi, N Yu; Muramatsu, H; Musiol, P; Nefedov, Y; Nerling, F; Nikolaev, I B; Ning, Z; Nisar, S; Niu, S L; Niu, X Y; Olsen, S L; Ouyang, Q; Pacetti, S; Pan, Y; Patteri, P; Pelizaeus, M; Peng, H P; Peters, K; Pettersson, J; Ping, J L; Ping, R G; Poling, R; Prasad, V; Qi, H R; Qi, M; Qian, S; Qiao, C F; Qin, L Q; Qin, N; Qin, X S; Qin, Z H; Qiu, J F; Rashid, K H; Redmer, C F; Ripka, M; Rong, G; Rosner, Ch; Ruan, X D; Sarantsev, A; Savri, M; Schnier, C; Schoenning, K; Schumann, S; Shan, W; Shao, M; Shen, C P; Shen, P X; Shen, X Y; Sheng, H Y; Shi, M; Song, W M; Song, X Y; Sosio, S; Spataro, S; Sun, G X; Sun, J F; Sun, S S; Sun, X H; Sun, Y J; Sun, Y Z; Sun, Z J; Sun, Z T; Tang, C J; Tang, X; Tapan, I; Thorndike, E H; Tiemens, M; Uman, I; Varner, G S; Wang, B; Wang, B L; Wang, D; Wang, D Y; Wang, K; Wang, L L; Wang, L S; Wang, M; Wang, P; Wang, P L; Wang, S G; Wang, W; Wang, W P; Wang, X F; Wang, Y; Wang, Y D; Wang, Y F; Wang, Y Q; Wang, Z; Wang, Z G; Wang, Z H; Wang, Z Y; Wang, Z Y; Weber, T; Wei, D H; Wei, J B; Weidenkaff, P; Wen, S P; Wiedner, U; Wolke, M; Wu, L H; Wu, L J; Wu, Z; Xia, L; Xia, L G; Xia, Y; Xiao, D; Xiao, H; Xiao, Z J; Xie, Y G; Xiu, Q L; Xu, G F; Xu, J J; Xu, L; Xu, Q J; Xu, Q N; Xu, X P; Yan, L; Yan, W B; Yan, W C; Yan, Y H; Yang, H J; Yang, H X; Yang, L; Yang, Y X; Ye, M; Ye, M H; Yin, J H; Yu, B X; Yu, C X; Yu, J S; Yuan, C Z; Yuan, W L; Yuan, Y; Yuncu, A; Zafar, A A; Zallo, A; Zeng, Y; Zeng, Z; Zhang, B X; Zhang, B Y; Zhang, C; Zhang, C C; Zhang, D H; Zhang, H H; Zhang, H Y; Zhang, J; Zhang, J J; Zhang, J L; Zhang, J Q; Zhang, J W; Zhang, J Y; Zhang, J Z; Zhang, K; Zhang, L; Zhang, S Q; Zhang, X Y; Zhang, Y; Zhang, Y H; Zhang, Y N; Zhang, Y T; Zhang, Yu; Zhang, Z H; Zhang, Z P; Zhang, Z Y; Zhao, G; Zhao, J W; Zhao, J Y; Zhao, J Z; Zhao, Lei; Zhao, Ling; Zhao, M G; Zhao, Q; Zhao, Q W; Zhao, S J; Zhao, T C; Zhao, Y B; Zhao, Z G; Zhemchugov, A; Zheng, B; Zheng, J P; Zheng, W J; Zheng, Y H; Zhong, B; Zhou, L; Zhou, X; Zhou, X K; Zhou, X R; Zhou, X Y; Zhu, K; Zhu, K J; Zhu, S; Zhu, S H; Zhu, X L; Zhu, Y C; Zhu, Y S; Zhu, Z A; Zhuang, J; Zotti, L; Zou, B S; Zou, J H

    2016-01-01

    By analyzing $2.93\\ \\rm fb^{-1}$ of data taken at the $\\psi(3770)$ resonance peak with the BESIII detector, we measure the branching fractions for the hadronic decays $D^+\\to K^0_SK^0_S K^+$, $D^+\\to K^0_SK^0_S \\pi^+$, $D^0\\to K^0_S K^0_S$ and $D^0\\to K^0_SK^0_SK^0_S$. They are determined to be ${\\mathcal B}(D^+\\to K^0_SK^0_SK^+)=(2.54 \\pm 0.05_{\\rm stat.} \\pm 0.12_{\\rm sys.})\\times 10^{-3}$, ${\\mathcal B}(D^+\\to K^0_SK^0_S\\pi^+)=(2.70 \\pm 0.05_{\\rm stat.} \\pm 0.12_{\\rm sys.})\\times 10^{-3}$, ${\\mathcal B}(D^0\\to K^0_SK^0_S)=(1.67 \\pm 0.11_{\\rm stat.} \\pm 0.11_{\\rm sys.})\\times 10^{-4}$ and ${\\mathcal B}(D^0\\to K^0_SK^0_SK^0_S)=(7.21 \\pm 0.33_{\\rm stat.} \\pm 0.44_{\\rm sys.})\\times 10^{-4}$, where the second one is measured for the first time and the others are measured with significantly improved precision over the previous measurements.

  7. Camilo Torres y el memorial de agravios

    Directory of Open Access Journals (Sweden)

    Carlos Lleras de la Fuente

    1959-12-01

    Full Text Available "¡Igualdad! Santo derecho de la igualdad: justicia que estribas en esto y en dar a cada uno lo que es suyo". Es con esta hermosa frase, digna del más egregio jurisconsulto romano, que encabeza Camilo Torres el párrafo final del Memorial de Agravios.

  8. Torre de televisión, Stuttgart

    Directory of Open Access Journals (Sweden)

    Leonhardt, F.

    1957-11-01

    Full Text Available Descripción general y estudio estático de la torre de televisión, construida en Stuttgart, de unos 310 m de altura total —antena metálica incluida— y provista de una especie de tronco de cono invertido, de mayor diámetro medio que el del fuste de la torre que, situado en la parte superior de la torre entre los 138 y 150 m de altura respecto al suelo, se ha subdividido, en altura, formando cuatro plantas, en las que se han alojado los servicies y un restaurante. Por encima de este cuerpo superior troncocónico se ha construido una terraza para poder disfrutar de espléndidas vistas panorámicas. La torre propiamente dicha tiene 10,8 m de diámetro en la base y 5,04 m en la parte superior, con una altura de 138 m entre estas dos secciones extremas.

  9. Nueva York: torres que rematan manzanas

    OpenAIRE

    Cattani, Rudivan Luiz

    2009-01-01

    La falta de luz y ventilación naturales provocada por las inmensas sombras de los nuevos edificios elevados del Downtown dio lugar a la ley de zonificación de 1916. La normativa reguló alturas, retrocesos aéreos de las torres y la división en tres zonas, residencial, comercial e industrial. El edificio escalonado (torta de boda) fue el resultado directo de la aplicación de esta nueva regulación. La construcción vertical del Downtown resultaba inevitable, pues no se disponía de superficie a...

  10. Jaime Torres Bodet: Centenario de su Natalicio (Jaime Torres Bodet: 100th Anniversary of His Birth).

    Science.gov (United States)

    Revista Interamericana de Educacion de Adultos, 2002

    2002-01-01

    Articles in this issue, written in Spanish, focus on the following: the philosophy of Jaime Torres Bodet (humanistic vision of adult education; objectives of public education in Mexico; Mexico and the issue of culture; The Mexican National Museum of History; Enrique Gonzalez Martinez, poet of all hours; Marti, Cuba's champion; educational…

  11. [Leonardo Torres Quevedo: the most prodigious inventor of his time].

    Science.gov (United States)

    de Posada, Francisco González

    2007-01-01

    Leonardo Torres Quevedo's contribution to "the conquest of the air", to which humanity devoted so much effort in the early twentieth century is describe and his original inventions on dirigibles -the "Torres Quevedo", the "Astra-Torres" and the "Coastal"- and cable cars - Monte Ulía (San Sebastián) y Spanish Aerocar (Niágara, Canáda) are emphasised.

  12. Eduardo Torres Quintero: hombre y mito

    Directory of Open Access Journals (Sweden)

    Gustavo Páez Escobar

    1979-06-01

    Full Text Available Buscándole título a esta nota he demorado un homenaje que traigo en mente hace buen tiempo a la memoria de Eduardo Torres Quintero, muerto en Tunja, la tierra de sus luchas y de sus sueños, el 10 de mayo de 1973. Para mí el rótulo de un escrito es definitivo. Si coincide con mis vibraciones cerebrales, la materia se vuelve maleable y acaso logre también hallar dúctil el pensamiento; y si no consigo acuñar la inscripción mágica, la que incite el nervio preciso, las ideas se escaparán esquivas y volátiles.

  13. La torre del Danubio, en Viena

    Directory of Open Access Journals (Sweden)

    Schmidt, Wilhelm

    1965-04-01

    Full Text Available The symbolic tower of the horticultural exhibition, held recently in Vienna, is a 260 m high structure made of reinforced concrete. Of the total height, 181 m constitute the stem, and the rest is made up of a metallic needle. Inside the tower there are two lifts, each with capacity for 15 people, and a travelling velocity of 6.5 m/sec. In the top 31 ms of the stem there are two terraces, a bar and a restaurant: they provide a wonderful view of the city and the surrounding district. The vertical outline of the stem is an arc of a parabola. A sliding formwork has been utilized for its construction, and a height of 4 m has been concreted every day: this has meant a movement of 25 cm of the formwork every hour. The tower has a weight of 17000 tons, which imposes large loads on the foundation. To withstand these, the foundations have been made in the form of a truncated cone, with a cylindrical hollow core. The base of the cone has an external diameter of 31 m, and an internal diameter of 10.20 m. A compression hoop strengthens the joint between the foundation cone and the stem itself. Although this tower is located in the centre of the city, it harmonizes very well with the surrounding buildings.La torre simbólica de la Exposición de Horticultura, celebrada recientemente en Viena (Austria, es de hormigón armado y tiene 260 m de altura total, de los que 181 corresponden al fuste y el resto a una aguja metálica. En el interior del fuste se han instalado dos ascensores para 15 personas cada uno, con una velocidad de 6,5 m por segundo. En los últimos 31 m se han montado terrazas, un bar y un restaurante, constituyendo las primeras unos magníficos miradores para contemplar la ciudad y sus alrededores- Dicho fuste es de hormigón armado y tiene un perfil en arco de parábola. Su construcción se ha realizado utilizando encofrados deslizantes. Se hormigonó a un ritmo de 4 m por día y una velocidad de corrimientos de los encofrados de 0,25 m por

  14. Pharmacological modulation of SK3 channels

    DEFF Research Database (Denmark)

    Grunnet, M; Jespersen, Thomas; Angelo, K

    2001-01-01

    of 512 microM and 6 microM, respectively) at concentrations lower than those used for brain slice recordings. Riluzole, a potent neuroprotective drug with anti-ischemic, anticonvulsant and sedative effects currently used in the treatment of amyotrophic lateral sclerosis, activates SK3 channels...

  15. Antiarrhythmic Mechanisms of SK Channel Inhibition in the Rat Atrium

    DEFF Research Database (Denmark)

    Skibsbye, Lasse; Wang, Xiaodong; Axelsen, Lene Nygaard

    2015-01-01

    remains unclear. OBJECTIVES: We speculated that together with a direct inhibition of repolarizing SK current, the previously observed depolarization of the atrial resting membrane potential (RMP) after SK channel inhibition reduces sodium channel availability thereby prolonging the effective refractory...

  16. Skærmens samfund/Screen Society

    DEFF Research Database (Denmark)

    Fausing, Bent

    Udgivelsen skal komme dels i en danks udgave og i en engelsk oversættelse. Der er skærme overalt i samfundet. Det er vanskeligere at finde et rum uden end et med en skærm. Hvad betyder det, at vi får oplevelser via skærme? Hvordan er skærmens æstetik og dens visualiseringer? Hvordan interagerer v...

  17. Reading the Planetary Archipelago of the Torres Strait

    Directory of Open Access Journals (Sweden)

    Elizabeth McMahon

    2013-05-01

    Full Text Available This essay makes use of the Western concept of the archipelago as a starting point for an examination of island to island relations in the Torres Strait, Australia, as they are rehearsed in the imaginary domain of story, in both its written and oral modes. The essay deploys Spivak’s notions of planetarity and Bloch’s concept of utopianism as ways of charting the relationship between two Torres Strait stories, one old, one new. In so doing, the essay seeks to identify the capacity of Torres Strait literature and storytelling to re-conceive relations of space and time and to acknowledge a spatial and temporal mobility running parallel to the circumscribed trajectories of late modernity.

  18. Cimentación de una torre deshidratadora mediante pilotes

    OpenAIRE

    Hormigo Delgado, Alberto

    2010-01-01

    El presente proyecto tiene por objeto la realización de la cimentación para el sustento de una torre deshidratadora que formará parte de las instalaciones necesarias del complejo industrial de Cepsa Química Guadarranque, permitiendo la obtención de Ácido Tereftálico Purificado (PTA).

  19. 7SK-BAF axis controls pervasive transcription at enhancers.

    Science.gov (United States)

    Flynn, Ryan A; Do, Brian T; Rubin, Adam J; Calo, Eliezer; Lee, Byron; Kuchelmeister, Hannes; Rale, Michael; Chu, Ci; Kool, Eric T; Wysocka, Joanna; Khavari, Paul A; Chang, Howard Y

    2016-03-01

    RNA functions at enhancers remain mysterious. Here we show that the 7SK small nuclear RNA (snRNA) inhibits enhancer transcription by modulating nucleosome position. 7SK occupies enhancers and super enhancers genome wide in mouse and human cells, and it is required to limit enhancer-RNA initiation and synthesis in a manner distinct from promoter pausing. Clustered elements at super enhancers uniquely require 7SK to prevent convergent transcription and DNA-damage signaling. 7SK physically interacts with the BAF chromatin-remodeling complex, recruits BAF to enhancers and inhibits enhancer transcription by modulating chromatin structure. In turn, 7SK occupancy at enhancers coincides with that of Brd4 and is exquisitely sensitive to the bromodomain inhibitor JQ1. Thus, 7SK uses distinct mechanisms to counteract the diverse consequences of pervasive transcription that distinguish super enhancers, enhancers and promoters.

  20. Solar Neutrino Measurement at SK-III

    CERN Document Server

    Yang, B S

    2009-01-01

    The full Super-Kamiokande-III data-taking period, which ran from August of 2006 through August of 2008, yielded 298 live days worth of solar neutrino data with a lower total energy threshold of 4.5 MeV. During this period we made many improvements to the experiment's hardware and software, with particular emphasis on its water purification system and Monte Carlo simulations. As a result of these efforts, we have significantly reduced the low energy backgrounds as compared to earlier periods of detector operation, cut the systematic errors by nearly a factor of two, and achieved a 4.5 MeV energy threshold for the solar neutrino analysis. In this presentation, I will present the preliminary SK-III solar neutrino measurement results.

  1. La correspondencia de Camilo Torres y Radio Sutatenza, 1962

    Directory of Open Access Journals (Sweden)

    Fernán González

    2014-02-01

    Full Text Available La correspondencia cruzada entre el padre Camilo Torres, decano entonces de la Escuela Superior de Administración Pública (ESAP, los monseñores José Joaquín Salcedo y Jorge Monastoque y el sociólogo Alejandro Bernal, de Radio Sutatenza, ilustran las tensiones que empezaban a surgir en el seno de la Iglesia católica en los años sesenta, que esa institución estaba muy lejos de ser internamente homogénea. Para los interesados en la biografía de Camilo Torres, este cruce de cartas muestra cómo él va endureciendo sus posiciones, que pasan de análisis críticos sobre la realidad nacional a una actitud de mayor compromiso político, al tiempo que va marcando distancias importantes frente a algunos sectores del clero.

  2. Literatura y pintura. Jusep Torres Campanals, una novela cubista

    Directory of Open Access Journals (Sweden)

    Vílchez Ruiz, Carmen E

    2007-08-01

    Full Text Available This article presents a study of the relation between the arts of literature and painting, from the interdisciplinary point of view. Focused on the structural analysis of Max Aub’s novel Jusep Torres Campanals, we will see how this work represents the translation of cubist painting’s aesthetics into the literary discourse. Through this analysis we will be able to prove how the narrative discourse’s decompositive technique, as used in a cubist painting, shows Jusep Torres Campanals as a fascinating dialogic dimension between truth and falseness, between painting and writing. Setting the reader again before the reflection of the old dialogue between literature and painting.Este artículo se presenta desde la perspectiva interdisciplinar del estudio de las relaciones entre dos artes, la Literatura y la Pintura, centrándose en el análisis estructural de la novela de Max Aub, Jusep Torres Campanals, tras el que observaremos cómo esta obra supone la traslación de la estética de la pintura cubista al discurso literario. A lo largo del análisis podremos comprobar, pues, cómo la técnica de descomposición del discurso narrativo, a la manera de un cuadro cubista, convierte a Jusep Torres Campanals, en un fascinante espacio de diálogo entre la verdad y la mentira, la pintura y la escritura, situándonos de nuevo ante la reflexión del antiguo diálogo entre la Literatura y la Pintura.

  3. Shared medical appointments for Aboriginal and Torres Strait Islander men.

    Science.gov (United States)

    Stevens, John A; Dixon, John; Binns, Andrew; Morgan, Bob; Richardson, Jeff; Egger, Garry

    2016-06-01

    Aboriginal and Torres Strait Islander health is generally the worst of any population group in Australia. Inaccessibility to health services is one possible cause of this. Shared medical appointments (SMAs) appear to be a culturally competent and appropriate way of improving access to, and the quality of, primary healthcare services for Aboriginal and Torres Strait Islander peoples. The objective of this article is to assess the acceptability and appropriateness of SMAs as an adjunct process in primary care for Aboriginal and Torres Strait Islander men. As part of a broader study on SMAs, three SMA sessions were delivered at an Aboriginal men's health centre in northern New South Wales. One-day training sessions in SMA facilitation were also provided to two groups of 12-14 Aboriginal health workers (AHWs). Mixed methods were used to assess patient and provider satisfaction, subjective outcomes, and operational procedures in the SMA groups, as well as interest in the SMA process by AHWs. Satisfaction with SMAs among Aboriginal men was unanimously positive, with the numbers in the group increasing over time. Patients most enjoyed the 'yarn up' nature of SMAs with peer support, which reduced the 'scary' and culturally 'unnatural' nature of one-on-one consultations with a general practitioner (GP). AHWs who were trained to a level to conduct SMAs saw this as an effective way of improving cultural competence in, and accessibility of, their various Aboriginal health services. The results, though not generalisable, suggest that SMAs may offer a culturally safe and appropriate tool to enhance Aboriginal and Torres Strait Islander peoples' access to primary care.

  4. Elektrooniline maksekäsk kiirendab kohtumenetlust / Marko Aavik

    Index Scriptorium Estoniae

    Aavik, Marko, 1977-

    2008-01-01

    Eesti kohtusüsteemist ning maksekäsumenetlusest. Ilmunud ka Valgamaalane 17. juuni 2008, lk. 2 ; Harju Ekspress 20. juuni 2008, lk. 6 ; Meie Maa 2. juuli 2008, lk. 5 ; Nädaline 1. juuli 2008, lk. 5 ; Severnoje Poberezhje 2. juuli 2008, lk. 2 (Melnitsõ pravosudija stali molot bõstreje) ; Sakala 18. juuli 2008, lk. 2 ; Vooremaa : Jõgeva Valla Teataja 4. okt. 2008, lk. 2 ; Meie Maa 8. okt. 2008, lk. 6, pealkiri kujul: e-maksekäsk lahendab võlanõude kiiresti ; Vooremaa 7. okt. 2008, lk. 2, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Koit 9. okt. 2008, lk. 3, pealkiri kujul: e-maksekäsk säästab aega ja raha ; Virumaa Teataja 9. okt. 2008, lk. 7, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Kuulutaja 10. okt. 2008, lk. 4, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Nädaline 14. okt. 2008, lk. 10, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Lääne Elu 14. okt. 2008, lk. 5, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Pärnu Postimees 18. okt. 2008, lk. 19, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti

  5. Elektrooniline maksekäsk kiirendab kohtumenetlust / Marko Aavik

    Index Scriptorium Estoniae

    Aavik, Marko, 1977-

    2008-01-01

    Eesti kohtusüsteemist ning maksekäsumenetlusest. Ilmunud ka Valgamaalane 17. juuni 2008, lk. 2 ; Harju Ekspress 20. juuni 2008, lk. 6 ; Meie Maa 2. juuli 2008, lk. 5 ; Nädaline 1. juuli 2008, lk. 5 ; Severnoje Poberezhje 2. juuli 2008, lk. 2 (Melnitsõ pravosudija stali molot bõstreje) ; Sakala 18. juuli 2008, lk. 2 ; Vooremaa : Jõgeva Valla Teataja 4. okt. 2008, lk. 2 ; Meie Maa 8. okt. 2008, lk. 6, pealkiri kujul: e-maksekäsk lahendab võlanõude kiiresti ; Vooremaa 7. okt. 2008, lk. 2, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Koit 9. okt. 2008, lk. 3, pealkiri kujul: e-maksekäsk säästab aega ja raha ; Virumaa Teataja 9. okt. 2008, lk. 7, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Kuulutaja 10. okt. 2008, lk. 4, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Nädaline 14. okt. 2008, lk. 10, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Lääne Elu 14. okt. 2008, lk. 5, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti ; Pärnu Postimees 18. okt. 2008, lk. 19, pealkiri kujul: e-maksekäsk lahendab võlanõuded kiiresti

  6. Charting Class ${\\cal S}_k$ Territory

    CERN Document Server

    Franco, Sebastian; Uranga, Angel

    2015-01-01

    We extend the investigation of the recently introduced class ${\\cal S}_k$ of 4d $\\mathcal{N}=1$ SCFTs, by considering a large family of quiver gauge theories within it, which we denote $\\mathcal{S}^1_k$. These theories admit a realization in terms of $\\mathbb{Z}_k$ orbifolds of Type IIA configurations of D4-branes stretched among relatively rotated sets of NS-branes. This fact permits a systematic investigation of the full family, which exhibits new features such as non-trivial anomalous dimensions differing from free field values and novel ways of gluing theories. We relate these ingredients to properties of compactification of the 6d (1,0) superconformal ${\\cal T}_N^k$ theories on spheres with different kinds of punctures. We describe the structure of dualities in this class of theories upon exchange of punctures, including transformations that correspond to Seiberg dualities, and exploit the computation of the superconformal index to check the invariance of the theories under them.

  7. Sistema de Torre de Refrigeração

    OpenAIRE

    Vinha, João Celso Rosa da

    2010-01-01

    Mestrado em Engenharia Química - Ramo Optimização Energética na Indústria Química Uma vez chegado o momento de elaborar uma tese para a conclusão do segundo ciclo de estudos em Engenharia Química, a escolha dentro das opções disponíveis, foi por um lado o contexto laboral, onde me insiro, como trabalhador estudante e por outro lado um tema relacionado com água. Surge então, como oportunidade de trabalho a avaliação do desempenho de uma Torre de Arrefecimento. Os objectivos foram definid...

  8. CABO DE RAFAEL TORRES OSORIO [Material gráfico

    OpenAIRE

    Anonymous

    1980-01-01

    EL ESPECIALISTA ALEJANDRO C. MORENO Y MARRERO LE ATRIBUYE EL DISEÑO DEL AJEDREZADO TRIPLE, EL AJEDREZADO EN MUESCAS Y EL AJEDREZADO PIRAMIDAL. PARA LA CONFECCIÓN DE LA DECORACIÓN DEL CABO SE EMPLEAN UNAS SERIES DE AÑILLAS A LAS QUE SE LES REALIZAN INCISIONES E INCRUSTACIONES FORMANDO LAS FIGURAS DESEADAS POR EL MAESTRO. RAFAEL TORRES OSORIO DESTACÓ POR SU INACABABLE CREATIVIDAD. Copia digital. Madrid: Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibl...

  9. Torre de Valencia - Madrid – España

    Directory of Open Access Journals (Sweden)

    Carvajal, J.

    1974-02-01

    Full Text Available This unique building, which occupies a privileged position opposite the Retiro Park, the most important green area inside the town, has been organized like a tower, attending mainly to the special reasons of its location, in order to obtain excellent views for the dwellings which occupy it, and the least possible shade of the surrounding areas. It is made up of a basement element, with three commercial and office floors over ground level and four one commercial and three for garages, lumber rooms and facilities - underneath same. The 25 storeys of the tower are designed for dwelling with varying surfaces. Vertical metal structure and reinforced concrete screns, in staircase boxes, and flat pugging, without girders.Este edificio singular, que ocupa una situación privilegiada frente al Retiro, la más importante zona verde interior de la ciudad, se ha organizado en forma de torre, atendiendo, principalmente, a sus especiales razones de emplazamiento, a fin de lograr unas inmejorables vistas a las viviendas que lo ocupan, y un menor sombreamiento a las zonas circundantes. Consta de un elemento basamental, con tres plantas comerciales y de oficinas sobre el nivel del suelo y cuatro —una comercial y tres de garajes, trasteros e instalaciones— por debajo del mismo. Las 25 plantas de la torre están destinadas a viviendas de superficies diversas. Estructura vertical metálica y de pantallas de hormigón armado, en cajas de escalera, y forjados planos, sin vigas.

  10. Muir-Torre Syndrome: case report and molecular characterization

    Directory of Open Access Journals (Sweden)

    Carolina Alejandra Rios

    Full Text Available CONTEXT: Muir-Torre syndrome is a rare autosomal dominant genodermatosis caused by mutations in the mismatch repair genes. It is characterized by the presence of sebaceous skin tumors and internal malignancies, affecting mainly the colon, rectum and urogenital tract. Awareness of this syndrome among physicians can lead to early diagnosis of these malignancies and a better prognosis. CASE REPORT: We report the case of a Chilean patient who, over the course of several years, had multiple skin lesions, endometrial cancer and colon cancer. The syndrome was diagnosed using molecular techniques such as microsatellite instability analysis, immunohistochemistry and DNA sequencing, which allowed us to find the causative mutation. CONCLUSION: Molecular diagnostics is a highly useful tool, since it allows clinicians to confirm the presence of mutations causing Muir-Torre syndrome. It is complementary to the analysis of the clinical data, such as dermatological presentation, presence of visceral malignancies and family history of colorectal tumors, and it provides important knowledge to help physicians and patients choose between treatment options.

  11. Skønhed - En engel gik forbi

    DEFF Research Database (Denmark)

    Jørgensen, Dorthe

    Hvorfor spilde tiden på skønhed i en verden fuld af problemer? Med dette provokerende spørgsmål indleder filosof og idéhistoriker Dorthe Jørgensen sin undersøgelse af, hvad skønhed er, og hvordan den kan hjælpe os i vor søgen efter det gode liv. Hun fortæller ikke bare om, hvad kunstnere og tænke...

  12. Skønhed - En engel gik forbi

    DEFF Research Database (Denmark)

    Jørgensen, Dorthe

    Hvorfor spilde tiden på skønhed i en verden fuld af problemer? Med dette provokerende spørgsmål indleder filosof og idéhistoriker Dorthe Jørgensen sin undersøgelse af, hvad skønhed er, og hvordan den kan hjælpe os i vor søgen efter det gode liv. Hun fortæller ikke bare om, hvad kunstnere og tænke...

  13. Los cuadros de costumbres de Torres Méndez

    Directory of Open Access Journals (Sweden)

    Jorge Moreno Clavijo

    1965-12-01

    Full Text Available El timbre Sol y luna distingue el excelente libro que el Banco Cafetero ha lanzado, en edición exigente de lujo, forrado en tela, con los cuadros de costumbres que firmara, a mediados del siglo pasado, ese eximio pintor bogotano que se llamó Ramón Torres Méndez. La obra está integrada por cincuenta y cinco dibujos pertenecientes a la colección que reposa en el Museo Nacional. El regalo que la empresa privada, asociándose al fomento de la cultura en clara labor colombianista, hace a los amantes de los buenos libros, no puede ser más oportuno,  ahora, cuando parecen desdibujarse los perfiles de cuanto hasta hoy nos ha definido y caracterizado.

  14. A respeito das torres de defesa na cidade do Salvador

    OpenAIRE

    Santiago, Cybèle Celestino

    1989-01-01

    p. 99-115 As torres foram construções de defesa de grande importância no período medieval. O presente artigo faz um rebatimento entre os elementos que eram utilizados naquela época em outras partes do mundo e os que para cá foram transportados por ocasião da colonização do Brasil, dando ênfase ao antigo Forte de São Tiago de Água de Meninos, em Salvador, cujas prospecções arqueológicas poderão evidenciar concretamente, pela primeira vez em nosso País, a existência do elemento de defesa com...

  15. Las torres Puerta de Europa, Madrid-España

    Directory of Open Access Journals (Sweden)

    Domínguez del Castillo, Tomás

    1996-08-01

    Full Text Available The functionality is the main aspect of the urban area of the twin towers located at the end of the "Paseo de la Castellana" (Madrid. With a tilting of 15º over the vertical and crossing directions, such architectonic area is detached for its place and design of facilities and installations. The structural system is very complex due to the high technology applied for its construction, what made very difficult the ending of project. The "Puerta de Europa" towers will be used for office rental and they are (due to the structural system and quality of construction materials used one of the most important buildings due to not only to the quality of materials and design, but also due to their security and control centralized systems.

    La funcionalidad preside el conjunto urbanístico formado por las dos torres situadas al final del Paseo de la Castellana. Con una inclinación de 15º sobre su vertical y hacia un punto común, dicho conjunto arquitectónico destaca, sobre todo, por su ubicación, diseño de instalaciones y servicios. Se destaca la complejidad de su sistema estructural que, unida a la alta tecnología empleada en su construcción, dificultaron la consecución del proyecto. Las torres Puerta de Europa, destinadas al alquiler de oficinas, son unos edificios cuya terminación -con la diversidad de materiales constructivos empleados- constituye, en sí misma, una de las de más alta calidad del momento, con sistemas de control y seguridad centralizados e integrados.

  16. Al margen de las Obras de Francisco de la Torre

    Directory of Open Access Journals (Sweden)

    Pérez-Abadín Barro, Soledad

    2003-12-01

    Full Text Available Francisco de la Torre's Works, published by Quevedo in 1631, enclose a second part, described in later years as an «appendix», with translations that did not belong in the original project prepared by the author some decades before. The summary of the 1588 license for printing describes the work's content as «lyric and adonic verse, and bucolic», without mentioning the translations. The new date throws into question the participation of Almeida and Brocense in the preparation and transmission of the manuscript that came to Quevedo. Therefore, the volume of 1631 cannot be considered as an example of salmantin group's collective poetical essay. Related chronological considerations also throw new light on the relationship between the author and Fernando de Herrera.Las Obras de Francisco de la Torre, publicadas por Quevedo en 1631, incluyen una segunda parte, que más tarde se llamaría «apéndice», con traducciones ajenas al proyecto inicial preparado por el propio autor décadas antes. El sumario de la licencia de impresión de 1588 describe el contenido de la obra aprobada como «los versos líricos y adónicos y la bucólica», sin mencionar las traducciones. La nueva fecha lleva a cuestionar el protagonismo de Almeida y el Brócense en la preparación y transmisión del manuscrito que más tarde llegaría a Quevedo. El volumen de 1631, por lo tanto, no puede considerarse como un exponente de un ejercicio poético colectivo del círculo salmantino. Los datos cronológicos también permiten replantear la relación del autor con Fernando de Herrera.

  17. Tests to Reduce TorreCat™ Technology to Practice

    Energy Technology Data Exchange (ETDEWEB)

    Westover, Tyler [Idaho National Lab. (INL), Idaho Falls, ID (United States); Emerson, Rachel Marie [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-02-26

    Torrefaction is the thermal treatment of materials in the absence of oxygen in the temperature range of 200 to 300 °C and has been shown to improve handling and grinding properties, hydrophobicity, volatiles content, energy density, and combustion performance of renewable energy biomass feedstock materials. The disadvantages of torrefaction are its relative high cost compared to the low value input feedstock material and the energy that can be lost to volatized gases. This work will demonstrate a new technology developed by Advanced Torrefaction Systems (ATS), known as TorreCat™ Technology, that uses an oxidation catalyst in a closed system to combust and destroy volatile organic compounds (VOCs) and other byproducts produced in the torrefaction process. An oxidation catalyst is a substance, or a combination of substances, that accelerate the rate of a chemical reaction without being consumed by the reaction. Catalytic combustion is a reaction that occurs at temperatures 50% lower than traditional combustion, such that essentially no NOx is created. The output of the oxidation catalyst (flue gas) consists mainly of superheated steam and inert gases (carbon dioxide and nitrogen), which can be used for heat in the thermal treatment process. INL has previously developed a pilot-scale Continuous-Feed Thermal Treatment System (CFTTS) that has 10 kg/hr capacity but does not reform the flue gas to reduce environmental concerns or capture all available heat from the biomass material. Using the TorreCat™ technology in INL’s thermal treatment system will demonstrate increased thermal efficiencies during the treatment process as well as reduced environmental impact and clean-up costs. The objective of this project is to determine the effectiveness of the Torrecat™ technology to reform the flue gas and capture as much of its heat content as possible.

  18. Solar neutrino measurement in SK and larger detector

    Energy Technology Data Exchange (ETDEWEB)

    Koshio, Y. [Kamioka observatory, ICRR, Univ. of Tokyo, Higashi-Mozumi, Kamioka cho, Hida city, Gifu pref (Japan)

    2005-08-15

    The results of solar neutrino data from the first phase of Super-Kamiokande (SK-I) are presented. Super-Kamiokande can measure not only the solar neutrino flux but also its energy spectrum and time variations such as day-night and seasonal differences. The detail oscillation analysis using those information is appeared. Combining all the solar and KamLAND reactor data, the obtained oscillation parameters are determined as {delta}m{sup 2}=8.3x10{sup -5}eV{sup 2} and tan{sup 2}{theta}=0.38. The recent results in the second phase of SK (SK-II) are also presented, and its flux, spectrum and time variation are consistent with SK-I. The possibility of future water Cherenkov detectors, such as SK-III and Mega-ton class detector, are described. If the current neutrino oscillation parameter is assumed, its spectrum has deficit in the lower energy region. And the possibility of the day-night asymmetry is also discussed.

  19. Improving the Participation and Engagement of Aboriginal and Torres Strait Islander Students in Business Education

    Science.gov (United States)

    Dang, Thi Kim Anh; Vitartas, Peter; Ambrose, Kurt; Millar, Hayley

    2016-01-01

    Most Australian universities have among their goals to increase the number of Aboriginal and Torres Strait Islander students at their institutions. In the Australian higher education context, Aboriginal and Torres Strait Islander students are seriously under-represented, particularly in business education compared to other disciplines. An…

  20. How can GPs drive software changes to improve healthcare for Aboriginal and Torres Strait Islanders peoples?

    Science.gov (United States)

    Kehoe, Helen

    2017-01-01

    Changes to the software used in general practice could improve the collection of the Aboriginal and Torres Strait Islander status of all patients, and boost access to healthcare measures specifically for Aboriginal and Torres Strait Islander peoples provided directly or indirectly by general practitioners (GPs). Despite longstanding calls for improvements to general practice software to better support Aboriginal and Torres Strait Islander health, little change has been made. The aim of this article is to promote software improvements by identifying desirable software attributes and encouraging GPs to promote their adoption. Establishing strong links between collecting Aboriginal and Torres Strait Islander status, clinical decision supports, and uptake of GP-mediated health measures specifically for Aboriginal and Torres Strait Islander peoples - and embedding these links in GP software - is a long overdue reform. In the absence of government initiatives in this area, GPs are best placed to advocate for software changes, using the model described here as a starting point for action.

  1. Branching fraction and CP asymmetries of B0-->K0(S)K0(S)K0(S).

    Science.gov (United States)

    Aubert, B; Barate, R; Boutigny, D; Couderc, F; Karyotakis, Y; Lees, J P; Poireau, V; Tissserand, V; Zghiche, A; Grauges-Pous, E; Palano, A; Pompili, A; Chen, J C; Qi, N D; Rong, G; Wang, P; Zhu, Y S; Eigen, G; Ofte, I; Stugu, B; Abrams, G S; Borgland, A W; Breon, A B; Brown, D N; Button-Shafer, J; Cahn, R N; Charles, E; Day, C T; Gill, M S; Gritsan, A V; Groysman, Y; Jacobsen, R G; Kadel, R W; Kadyk, J; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lynch, G; Mir, L M; Oddone, P J; Orimoto, T J; Pripstein, M; Roe, N A; Ronan, M T; Wenzel, W A; Barrett, M; Ford, K E; Harrison, T J; Hart, A J; Hawkes, C M; Morgan, S E; Watson, A T; Fritsch, M; Goetzen, K; Held, T; Koch, H; Lewandowski, B; Pelizaeus, M; Peters, K; Schroeder, T; Steinke, M; Boyd, J T; Burke, J P; Chevalier, N; Cottingham, W N; Kelly, M P; Latham, T E; Wilson, F F; Cuhadar-Donszelmann, T; Hearty, C; Knecht, N S; Mattison, T S; McKenna, J A; Thiessen, D; Khan, A; Kyberd, P; Teodorescu, L; Blinov, A E; Blinov, V E; Druzhinin, V P; Golubev, V B; Ivanchenko, V N; Kravchenko, E A; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Yushkov, A N; Best, D; Bruinsma, M; Chao, M; Eschrich, I; Kirkby, D; Lankford, A J; Mandelkern, M; Mommsen, R K; Roethel, W; Stoker, D P; Buchanan, C; Hartfiel, B L; Weinstein, A J R; Foulkes, S D; Gary, J W; Long, O; Shen, B C; Wang, K; del Re, D; Hadavand, H K; Hill, E J; MacFarlane, D B; Paar, H P; Rahatlou, Sh; Sharma, V; Berryhill, J W; Campagnari, C; Cunha, A; Dahmes, B; Hong, T M; Lu, A; Mazur, M A; Richman, J D; Verkerke, W; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Nesom, G; Schalk, T; Schumm, B A; Seiden, A; Spradlin, P; Williams, D C; Wilson, M G; Albert, J; Chen, E; Dubois-Felsmann, G P; Dvoretskii, A; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Ryd, A; Samuel, A; Yang, S; Jayatilleke, S; Mancinelli, G; Meadows, B T; Sokoloff, M D; Blanc, F; Bloom, P; Chen, S; Ford, W T; Nauenberg, U; Olivas, A; Rankin, P; Ruddick, W O; Smith, J G; Ulmer, K A; Zhang, J; Zhang, L; Chen, A; Eckhart, E A; Harton, J L; Soffer, A; Toki, W H; Wilson, R J; Zeng, Q; Spaan, B; Altenburg, D; Brandt, T; Brose, J; Dickopp, M; Feltresi, E; Hauke, A; Lacker, H M; Maly, E; Nogowski, R; Otto, S; Petzold, A; Schott, G; Schubert, J; Schubert, K R; Schwierz, R; Sundermann, J E; Bernard, D; Bonnneaud, G R; Grenier, P; Schrenk, S; Thiebaux, Ch; Vasileiadis, G; Verderi, M; Bard, D J; Clark, P J; Muheim, F; Playfer, S; Xie, Y; Andreotti, M; Azzolini, V; Bettoni, D; Bozzi, C; Calabrese, R; Cibinetto, G; Luppi, E; Negrini, M; Piemontese, L; Sarti, A; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; de Sangro, R; Finocchiaro, G; Patteri, P; Peruzzi, I M; Piccolo, M; Zallo, A; Buzzo, A; Capra, R; Contri, R; Crosetti, G; Lo Vetere, M; Macri, M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Bailey, S; Brandenburg, G; Chaisanguanthum, K S; Morii, M; Won, E; Dubitzky, R S; Langenegger, U; Marks, J; Uwer, U; Bhimji, W; Bowerman, D A; Dauncey, P D; Egede, U; Gaillard, J R; Morton, G W; Nash, J A; Nikolich, M B; Taylor, G P; Charles, M J; Grenier, G J; Mallik, U; Mohapatra, A K; Cochran, J; Crawley, H B; Lamsa, J; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Yi, J; Arnaud, N; Davier, M; Giroux, X; Grosdidier, G; Höcker, A; Le Diberder, F; Lepeltier, V; Lutz, A M; Petersen, T C; Pierini, M; Plaszczynski, S; Schune, M H; Wormser, G; Cheng, C H; Lange, D J; Simani, M C; Wright, D M; Bevan, A J; Chavez, C A; Coleman, J P; Forster, I J; Fry, J R; Gabathuler, E; Gamet, R; Hutchhcroft, D E; Parry, R J; Payne, D J; Touramanis, C; Cormack, C M; Di Lodovico, F; Brown, C L; Cowan, G; Flack, R L; Flaecher, H U; Green, M G; Jackson, P S; McMahon, T R; Ricciardi, S; Salvatore, F; Winter, M A; Brown, D; Davis, C L; Allison, J; Barlow, N R; Barlow, R J; Hodgkinson, M C; Lafferty, G D; Naisbit, M T; Williams, J C; Chen, C; Farbin, A; Hulsbergen, W D; Jawahery, A; Kovalskyi, D; Lae, C K; Lillard, V; Roberts, D A; Blaylock, G; Dallapiccola, C; Hertzbach, S S; Kofler, R; Koptchev, V B; Moore, T B; Saremi, S; Staengle, H; Willocq, S; Cowan, R; Koeneke, K; Sciolla, G; Sekula, S J; Taylor, F; Yamamoto, R K; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Reidy, J; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Taras, P; Nicholson, H; Cavallo, N; Fabozzi, F; Gatto, C; Lista, L; Monorchio, D; Paolucci, P; Piccolo, D; Sciacca, C; Baak, M; Bulten, H; Raven, G; Snoek, H L; Wilden, L; Jessop, C P; LoSecco, J M; Allmendinger, T; Bennelli, G; Gan, K K; Honscheid, K; Hufnagel, D; Kagan, H; Kass, R; Pulliam, T; Rahimi, A M; Ter-Antonyan, R; Wong, Q K; Brau, J; Frey, R; Igonkina, O; Lu, M; Potter, C T; Sinev, N B; Strom, D; Torrence, E; Colecchia, F; Dorigo, A; Galeazzi, F; Margoni, M; Morandin, M; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Benayoun, M; Briand, H; Chauveau, J; David, P; Del Buono, L; de la Vaissière, Ch; Hamon, O; John, M J J; Leruste, Ph; Malclès, J; Ocariz, J; Roos, L; Therin, G; Behera, P K; Gladney, L; Guo, Q H; Panetta, J; Biasini, M; Covarelli, R; Pioppi, M; Angelini, C; Batignani, G; Bettarini, S; Bondioli, M; Bucci, F; Calderini, G; Carpinelli, M; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Morganti, M; Neri, N; Paoloni, E; Rama, M; Rizzo, G; Simi, G; Walsh, J; Haire, M; Judd, D; Paick, K; Wagoner, D E; Danielson, N; Elmer, P; Lau, Y P; Lu, C; Miftakov, V; Olsen, J; Smith, A J S; Telnov, A V; Bellini, F; Cavoto, G; D'Orazio, A; Di Marco, E; Faccini, R; Ferrarotto, F; Ferroni, F; Gaspero, M; Li Gioi, L; Mazzoni, M A; Morganti, S; Piredda, G; Polci, F; Safai Tehrani, F; Voena, C; Christ, S; Schröder, H; Wagner, G; Waldi, R; Adye, T; De Groot, N; Franek, B; Gopal, G P; Olaiya, E O; Aleksan, R; Emery, S; Gaidot, A; Ganzhur, S F; Giraud, P-F; Hamel de Monchenault, G; Kozanecki, W; Legendre, M; London, G W; Mayer, B; Vasseur, G; Yèche, Ch; Zito, M; Purohit, M V; Weidemann, A W; Wilson, J R; Yumiceva, F X; Abe, T; Aston, D; Bartoldus, R; Berger, N; Boyarski, A M; Buchmueller, O L; Claus, R; Convery, M R; Cristinziani, M; De Nardo, G; Dingfelder, J C; Dong, D; Dorfan, J; Dujmic, D; Dunwoodie, W; Fan, S; Field, R C; Glanzman, T; Gowdy, S J; Hadig, T; Halyo, V; Hast, C; Hrny'ova, T; Innes, W R; Kelsey, M H; Kim, P; Kocian, M L; Leith, D W G S; Libby, J; Luitz, S; Luth, V; Lynch, H L; Marsiske, H; Messner, R; Muller, D R; O'Grady, C P; Ozcan, V E; Perazzo, A; Perl, M; Ratcliff, B N; Roodman, A; Salnikov, A A; Schindler, R H; Schwiening, J; Snyder, A; Soha, A; Stelzer, J; Strube, J; Su, D; Sullivan, M K; Va'vra, J; Wagner, S R; Weaver, M; Wisniewski, W J; Wittgen, M; Wright, D H; Yarritu, A K; Young, C C; Burchat, P R; Edwards, A J; Majewski, S A; Petersen, B A; Roat, C; Ahmed, M; Ahmed, S; Alam, M S; Ernst, J A; Saeed, M A; Saleem, M; Wappler, F R; Bugg, W; Krishnamurthy, M; Spanier, S M; Eckmann, R; Kim, H; Ritchie, J L; Satpathy, A; Schwitters, R F; Izen, J M; Kitayama, I; Lou, X C; Ye, S; Bianchi, F; Bona, M; Gallo, F; Gamba, D; Bosisio, L; Cartaro, C; Cossutti, F; Della Ricca, G; Dittongo, S; Grancagnolo, S; Lanceri, L; Poropat, P; Vitale, L; Vuagnin, G; Martinez-Vidal, F; Panvini, R S; Banerjee, Sw; Bhuyan, B; Brown, C M; Fortin, D; Hamano, K; Jackson, P D; Kowalewski, R; Roney, J M; Sobie, R J; Back, J J; Harrison, P F; Mohanty, G B; Band, H R; Chen, X; Cheng, B; Dasu, S; Datta, M; Eichenbaum, A M; Flood, K T; Graham, M; Hollar, J J; Johnson, J R; Kutter, P E; Li, H; Liu, R; Mihalyi, A; Pan, Y; Prepost, R; Tan, P; von Wimmersperg-Toeller, J H; Wu, J; Wu, S L; Yu, Z; Greene, M G; Neal, H

    2005-07-01

    We present measurements of the branching fraction and time-dependent CP-violating asymmetries in B0-->K0(S)K0(S)K0(S) decays based on 227 x 10(6) Upsilon4S-->BB decays collected with the BABAR detector at the PEP-II asymmetric-energy B factory at SLAC. We obtain a branching fraction of (6.9(+0.9)(-0.8)+/-0.6) x 10(-6), and CP asymmetries C=-0.34(+0.28)(-0.25)+/-0.05 and S=-0.71(+0.38)(-0.32)+/-0.04, where the first uncertainties are statistical and the second systematic.

  2. DiamondTorre Algorithm for High-Performance Wave Modeling

    Directory of Open Access Journals (Sweden)

    Vadim Levchenko

    2016-08-01

    Full Text Available Effective algorithms of physical media numerical modeling problems’ solution are discussed. The computation rate of such problems is limited by memory bandwidth if implemented with traditional algorithms. The numerical solution of the wave equation is considered. A finite difference scheme with a cross stencil and a high order of approximation is used. The DiamondTorre algorithm is constructed, with regard to the specifics of the GPGPU’s (general purpose graphical processing unit memory hierarchy and parallelism. The advantages of these algorithms are a high level of data localization, as well as the property of asynchrony, which allows one to effectively utilize all levels of GPGPU parallelism. The computational intensity of the algorithm is greater than the one for the best traditional algorithms with stepwise synchronization. As a consequence, it becomes possible to overcome the above-mentioned limitation. The algorithm is implemented with CUDA. For the scheme with the second order of approximation, the calculation performance of 50 billion cells per second is achieved. This exceeds the result of the best traditional algorithm by a factor of five.

  3. Legally invisible: stewardship for Aboriginal and Torres Strait Islander health.

    Science.gov (United States)

    Howse, Genevieve; Dwyer, Judith

    2016-04-01

    The need to improve access to good health care for Aboriginal and Torres Strait Islander people has been the subject of policy debate for decades, but progress is hampered by complex policy and administrative arrangements and lack of clarity about the responsibilities of governments. This study aimed to identify the current legal basis of those responsibilities and define options available to Australian governments to enact enduring responsibility for Aboriginal health care. This study used a framework for public health law research and conducted a mapping study to examine the current legal underpinnings for stewardship and governance for Aboriginal health and health care. More than 200 pieces of health legislation were analysed in the context of the common and statutory law and health policy goals. Very little specific recognition of the needs of Aboriginal people was found, and nothing that creates responsibility for stewardship and governance. The continuing absence of a legislative framework to address and protect Aboriginal health can be traced back to the founding doctrine of terra nullius (unoccupied land). We considered the results applying both a human rights perspective and the perspective of therapeutic jurisprudence. We suggest that national law for health stewardship would provide a strong foundation for progress, and should itself be based on recognition of Australia's First Peoples in the Australian Constitution, as is currently proposed. © 2015 The Authors.

  4. Love(s in Clarice and Rui Torres

    Directory of Open Access Journals (Sweden)

    Keilla Conceição Petrin Grande

    2013-10-01

    Full Text Available This article, attempts to demonstrate one of several forms of dialogue between the portuguese literatures, proposes an anlysis of the short story Amor, by Clarice Lispector and the poem Amor de Clarice, by Rui Torres. Considering that the poem is from of a preexisting text, or an “hypotext”, as defined by Gennete, analyse these productions from the perspective of intersemiotic translation according the works of Julio Plaza and Claus Clüver, since these theorists treat the “translation” less as a transfer of meaning from the original, which should be subordinate to it, an activity which involves creating thus transforming. Also, the Torres’s (rewrinting proposes, as a poet, a way to make the contemporary literary criticism. Thus, this study will develop towards analyzing how text elements clariceano were (reproduced, (recreated, transformed in the poem Rui and how a work of twenty-first century can shed new questions, new perspectives and new reflections to text in the last century.

  5. Torre de iluminación y comunicaciones de Madrid

    Directory of Open Access Journals (Sweden)

    Pérez Arroyo, Salvador

    1992-06-01

    Full Text Available This article describes the communication and lighting tower of the Avenida de la Victoria in Madrid, a work belonging to the project of Madrid the Cultural Capital of Europe 1992. The aim of this construction is to light this road junction and, at the same time present a visual landmark of the city. The lantern-tower will light an area of about one hectare and will serve as a base for locating the traffic control cameras and other services.

    En este artículo se describe la farola de iluminación y comunicaciones de la Avenida de la Victoria, en Madrid, obra enmarcada dentro del Madrid Cultural de 1992. El objetivo de esta construcción es iluminar este nudo de confluencia vial y, al mismo tiempo, marcar un hito visual de la ciudad. La torre-farola ilumina una superficie no menor de una hectárea y es base para la ubicación de cámaras para el control del tráfico y otros servicios.

  6. Global features of the Alcanivorax borkumensis SK2 genome

    DEFF Research Database (Denmark)

    Reva, Oleg N.; Hallin, Peter Fischer; Willenbrock, Hanni

    2008-01-01

    The global feature of the completely sequenced Alcanivorax borkumensis SK2 type strain chromosome is its symmetry and homogeneity. The origin and terminus of replication are located opposite to each other in the chromosome and are discerned with high signal to noise ratios by maximal oligonucleot...

  7. En "skælvsættende" dom?

    DEFF Research Database (Denmark)

    Lauta, Kristian Cedervall

    2012-01-01

    Sanktioner imod bevidste undladelser eller uprofessionelle skøn er en måde at opretholde tilliden til ingeniører på, og det samme gælder seismologer. ... Sagen illustrerer de fundamentale forandringer, der de sidste 30 år er sket med vores forståelse af, hvad en ' naturkatastrofe' er. Katastrofer...

  8. Analysis of Future KamLAND and Gadolinium Doped SK

    Energy Technology Data Exchange (ETDEWEB)

    Choubey, Sandhya [INFN and SISSA, Trieste (Italy)

    2005-12-15

    We probe in detail the precision expected in the measurement of {delta}m{sub 21}{sup 2} and sin{sup 2}{theta}{sub 12} in the SNO, KamLAND, the proposed SK-Gd and a reactor experiment tuned to the SPMIN.

  9. Uncovering a Role for SK2 in Angelman Syndrome

    Directory of Open Access Journals (Sweden)

    Sofia B. Lizarraga

    2015-07-01

    Full Text Available Angelman syndrome is a severe neurodevelopmental disorder caused by mutations in UBE3A. Sun et al. (2015 report SK2 as a UBE3A substrate and provide insight into the molecular mechanisms that might underlie impaired neuronal function in individuals affected by Angelman syndrome.

  10. Identification and characterization of a novel, shorter isoform of the small conductance Ca(2+)-activated K(+) channel SK2

    NARCIS (Netherlands)

    Murthy, Saravana R. K.; Teodorescu, Georgeta; Nijholt, Ingrid M.; Dolga, Amalia; Grissmer, Stephan; Spiess, Joachim; Blank, Thomas

    2008-01-01

    Throughout the CNS, small conductance Ca(2+)-activated potassium (SK) channels modulate firing frequency and neuronal excitability. We have identified a novel, shorter isoform of standard SK2 (SK2-std) in mouse brain which we named SK2-sh. SK2-sh is alternatively spliced at exon 3 and therefore lack

  11. Torres de televisión: Evolución y desarrollo

    Directory of Open Access Journals (Sweden)

    del Solar Bermejo, José

    1982-06-01

    Full Text Available Not available.Las torres de telecomunicación pertenecen a un campo de la Arquitectura, profundamente ligado a la Ingeniería, de reciente aparición y enorme desarrollo tecnológico. Con la Introducción de la Televisión en el mundo es cuando estas torres han alcanzado un mayor auge, hasta el punto de haber generalizado su nombre al de una de las muchas funciones para las que se destinan, llamándolas normalmente Torres de Televisión. No obstante, torres de televisión puras son muy raras, siendo normal que se destinen a emisiones de radio, televisión, telegrafía y telefonía. Funclonalmente, todavía es conveniente distinguir entre las torres destinadas exclusivamente a transmisiones radioelèctricas y las que además poseen instalaciones de otro tipo, como plataformas de observación abiertas al público, restaurantes giratorios o no, oficinas, estudios de radiotelevisión o incluso instalaciones de observación meteorológica. En el diseño de la torre influye en gran medida el material empleado en su construcción. Bajo esa consideración, podemos distinguir dos grandes grupos de torres: de acero y de hormigón.

  12. Inmueble-torre ''Los horizontes'' Rennes – Francia

    Directory of Open Access Journals (Sweden)

    Maillols, Georges

    1975-09-01

    Full Text Available This building constitutes a part of the renovation operation in the «Distrito del Obispo», in the street of «Brest» at Rennes. The complex contains: 2,000 apartments, parking places for approximately 600 cars, shopping centre, social centre, schools, hotel restaurant, etc. The two towers that are being described specially in the article comprise: two basements, two mezzanine floors, thirty floors for housing and offices and two technical storeys; with a separate circular garage, surface parking place and a separate building for meetings. Foundation on piles of 1.50 diameter anchored in the bare rock: structure of reinforced concrete and prefabricated facades, with additional installations of all kinds to assure comfort and perfect functioning.Este edificio forma parte de la operación de renovar el «Distrito del Obispo» de la calle de Brest a Rennes. El conjunto comprende: 2.000 apartamentos, oficinas, aparcamientos para unos 600 automóviles, zona comercial, centro social, escuelas, hotel restaurante, etc. Las dos torres, que se describen especialmente en el artículo, tienen: dos sótanos, planta baja, dos entreplantas, treinta plantas para viviendas y oficinas, y dos plantas técnicas; con garaje circular separado, aparcamiento en superficie y edificio exento para reuniones. Cimentación a base de pilotes de 1,50 de diámetro, anclados en la roca sana; estructura de hormigón armado y fachadas prefabricadas, con toda suerte de instalaciones complementarias para asegurar el confort y el perfecto funcionamiento debidos.

  13. Reproductive biology of Tinantia anomala (Torr. C.B. Clarke

    Directory of Open Access Journals (Sweden)

    Joanna Gębura

    2014-04-01

    Full Text Available Tinantia anomala (Torr. C.B. Clarke (Commelinaceae is an annual plant endemic to central Texas in the United States and Durango in northern Mexico. T. anomala has zygomorphic flowers with three different petals, androecium consists of six various stamens and gynoecium consists of three carpels. Furthermore in T. anomala’s flowers there are many staminal hairs (Faden 2006. Its semi-succulent, grass-like leaves emerge in late fall and remain green throughout the cold months. It grows rapidly in early spring and blooms mid-spring (from March to May. A few weeks later the fruits (capsules with four seeds usually appear. This entire cycle is usually completed by summer, when the plant has often turned yellow and limp. We studied T. anomala due to the occurrence of two types of strikingly different stamens occur in one flower. According to available literature, one type of the stamens (the upper one with smaller anthers produces sterile pollen grains which do not participate in fertilization but only attract insects. The other type, i.e. the lower stamens with bigger anthers can form abundant pollen grains used for pollination (Simpson et al. 1986. Our aim was to investigate type of pollination of T. anomala. Using many microscopic methods we were able to analyze and compare the morphology of two types of anthers and staminal hairs in T. anomala’s flowers. We also investigated this species in terms of development of its gametophytes. An embryo sac develops according to the type of Polygonum. Pollen grains develop during different ways depending on the chemical composition of each anther. We identified that the insects of Thysanoptera which preys on the withered flowers T. anomala could be responsible for pollination of this species under greenhouse condition.

  14. High mountain soils and periglacial features at the Torres del Paine, National Park Torres del Paine, Chile.

    Science.gov (United States)

    Senra, Eduardo; Schaefer, Carlos; Simas, Felipe; Gjorup, Davi

    2015-04-01

    The Torres del Paine National Park (TPNP) is located on the southern limit of the Andean Southern Ice Field, part of the Magallanes and Antartica Chilena region, in the province of Ultima Esperanza. The TPNP has a very heterogeneous climate due to orographic influence and wet air masses from the Pacific. The geology is basically Cretaceous metasedimentary rocks and Miocene granitic plutons and batholiths. We studied the main soils and geoenvironments of Mt Ferrier mountain and its surroundings, based on soils , landforms and vegetation aspects. The geoenvironmental stratification was based on the combined variation and integration of pedo-litho-geomorphological features with the vegetation. WE used detailed geological maps, a DEM and slope maps and WorlView II satellite images. Fifteen soils profiles were sampled and classified according to Soil Taxonomy (2010) at all genovironments, ranging from 50 m a.s.l to the at high plateau just below the permanent snowline, under periglacial conditions (~1004m asl). Three soil temperature and moisture monitoring sites were set, allowing for 24 consecutive months (2011 to 2013). Seven geoenvironments were identified with distinct soil and landform characteristics, all with a similar geological substrate. The landform and vegetation have a strong connection with the landscape dynamic, controlling erosional and depositional processes, resulting from glacier advances and retreats in the Late Quaternary. Wind blown materials is widespread, in the form of loess material, accumulating in the higher parts of the landscape. On the other hand, accumulation of organic matter in the water-saturated depressions is common in all altitudes. Generally the soils are acidic and dystrophic, with little exceptions. The following geoenvironments were identified: Periglacial Tundra, Loess slopes, Talus and scarpmentd, Fluvio-glacial terraces, Fluvio-lacustrine plains, Moraines and Paleodunes. The regional pedology show the occurrence of five soil

  15. Cultural safety and maternity care for Aboriginal and Torres Strait Islander Australians.

    Science.gov (United States)

    Kruske, Sue; Kildea, Sue; Barclay, Lesley

    2006-09-01

    To discuss cultural safety and critique the provision of culturally appropriate maternity services to remote Aboriginal and Torres Strait Islander women in Australia. The literature and policies around 'culture' and 'cultural safety' are discussed and applied to the provision of maternity services to Aboriginal and Torres Strait Islander women in remote areas of Australia. The current provision of maternity services to Aboriginal and Torres Strait Islander women, particularly those living in remote Australia, appears largely inadequate. The provision of culturally safe maternity care requires health system reform at all levels including: the individual practitioner response; the educational preparation of practitioners; the delivery of maternity services and the development of policy at local, state and national level. This paper considers the changes that can be made from the individual practitioner through to the design and implementation of maternity services. Cultural safety provides a useful framework to improve the delivery of maternity services to remote Aboriginal and Torres Strait Islander women and their families.

  16. Characteristics of glycosylated streptokinase secreted from Pichia pastoris: enhanced resistance of SK to proteolysis by glycosylation.

    Science.gov (United States)

    Pratap, J; Rajamohan, G; Dikshit, K L

    2000-04-01

    Degradation of streptokinase (SK) has been frequently observed during large-scale protein production. An enhanced susceptibility of SK to degradation has been correlated with its existence in a partially unfolded state. The influence of the carbohydrate moiety on the stability and functional characteristics of SK has been examined by obtaining the glycoform of SK following its secretion through the methylotrophic yeast Pichia pastoris. Secretion of the protein product was achieved by replacing the native secretion signal codons of SK with those from alpha-factor leader peptide and expressing the fusion construct under the control of the methanol-inducible alcohol oxidase (ox) promoter of P. pastoris after its integration into the host chromosome. Western blot and zymographic analysis of proteins secreted from the recombinant P. pastoris indicated that SK was glycosylated by the host cells, which resulted in the appearance of a SK species migrating slowly, corresponding to a 55-kDa protein product as compared to the 47-kDa native SK. The glycosylated SK retained a plasminogen activation capability identical to that of its unglycosylated counterpart. Glycoform SK exhibited an enhanced stability profile at 25 degrees C and 37 degrees C and improved resistance towards protease treatment compared to unglycosylated SK secreted through P. pastoris after tunicamycin treatment or that secreted from the recombinant Escherichia coli. The results presented thus illustrate that N-linked glycosylation of SK results in 30-40% enhancement of the protein stability and resistance towards degradation but does not interfere with its fibrinolytic function.

  17. File list: His.Brs.20.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Brs.20.AllAg.SK-BR-3 hg19 Histone Breast SK-BR-3 SRX750665,SRX750660,SRX469107,...SRX469108 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Brs.20.AllAg.SK-BR-3.bed ...

  18. File list: Oth.Brs.10.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.10.AllAg.SK-BR-3 hg19 TFs and others Breast SK-BR-3 SRX155755,SRX128102,SRX...155754 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Brs.10.AllAg.SK-BR-3.bed ...

  19. File list: Oth.Brs.20.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.20.AllAg.SK-BR-3 hg19 TFs and others Breast SK-BR-3 SRX155755,SRX128102,SRX...155754 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Brs.20.AllAg.SK-BR-3.bed ...

  20. File list: His.Brs.05.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Brs.05.AllAg.SK-BR-3 hg19 Histone Breast SK-BR-3 SRX750660,SRX750665,SRX469108,...SRX469107 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Brs.05.AllAg.SK-BR-3.bed ...

  1. File list: Oth.Brs.50.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.50.AllAg.SK-BR-3 hg19 TFs and others Breast SK-BR-3 SRX128102,SRX155755,SRX...155754 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Brs.50.AllAg.SK-BR-3.bed ...

  2. File list: Oth.Brs.05.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Brs.05.AllAg.SK-BR-3 hg19 TFs and others Breast SK-BR-3 SRX155754,SRX155755,SRX...128102 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Brs.05.AllAg.SK-BR-3.bed ...

  3. File list: His.Brs.10.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available His.Brs.10.AllAg.SK-BR-3 hg19 Histone Breast SK-BR-3 SRX750660,SRX750665,SRX469108,...SRX469107 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/His.Brs.10.AllAg.SK-BR-3.bed ...

  4. The legacy of Maria Curie Skłodowska

    Science.gov (United States)

    Sosnowski, Ryszard

    2011-01-01

    Maria Skłodowska Curie left us a great legacy. Her discovery of polonium and radium was incomparably greater than the mere discovery of new elements. Its significance lay in the discovery of a new form of matter, namely radioactive one, but also in her unveiling of the internal property of its atoms. Subsequently emitted radiation went on to play the role of a "natural accelerator" for both scientific research and in medical radiotherapy. It was thanks to these discoveries that the field of nuclear physics arose just a few decades later. As importantly the work of Maria Curie Skłodowska during the Great War demonstrated how important pure scientific discovery can be for society and its welfare.

  5. ARQUEOLOGIA E IDENTIDADES: A TORRE DE MARFIM NA ENCRUZILHADA

    Directory of Open Access Journals (Sweden)

    Luiz Oosterbeek

    2012-09-01

    Full Text Available A arqueologia cresceu, desde o século XIX, no equívoco epistemológico de se assumir ora como corpo de técnicas, ora como forma especifica de fazer história, mas sempre como um corpo cuja dignidade disciplinar se foi, com algumas excepções, procurando na construção de “segredos da profissão”. No início do século XXI, essa torre de marfim, há muito feita ruína, vai ganhando consciência de que não tem futuro, ou de que o seu futuro exige uma abertura social, o assumir do seu carácter supra-disciplinar, e o contributo que, nessa dimensão, diferentes sectores da sociedade devem dar para a construção do saber arqueológico. Em face deuma lógica de “resistência” (o patrimonialista é o que resiste á vaga de destruição da memória, propõe-se uma abordagem holística e “agressiva”, que assume o arqueólogo como técnico ao serviço da sociedade. Mas, como tudo na vida, há um preço a pagar....e sobre ele se tentará conversar um pouco.PALAVRAS-CHAVE: arqueologia; patrimônio; turismo;qualidade. ABSTRACT: Archaeology grew, throughout the XIXth century, divided between considering itself as a corpus of techniques or as a specific way of making history, but always as a common "body" with a shared disciplinary dignity based, with few exception, in the building of "professional secrets". In the dawn of the XXIst century this ivory tower, long turned into a ruin, is becoming aware of its lack of future, or that its future requires social openness, assuming a character beyond disciplines, and that, in such dimension, different social sectors need to provide specific contributions to the construction of the archaeological knowledge. Facing a "resistance" logics (the heritage professional is the one who resistsagainst memory loss, one suggests an holistic and "aggressive" approach, assuming the archaeologist as a technician serving society. But, as always in life, there's a price to pay...one we will

  6. Hotel de la Torre Londres - Gran Bretaña

    Directory of Open Access Journals (Sweden)

    Howard Wood, Renton

    1978-04-01

    Full Text Available It has been built on the banks of the Thames, and owes its name to its nearness to the Tower of London, which also gave its name to the old Tower Bridge, located over the river and a few metres from the building. The rear frontage of the hotel faces the nearby Saint Catherine's Dock, completing an attractive surrounding in one of the paradoxically least developed areas of the city, and which has been taken least advantage of. Total construction occupies an area of 36,560 m2 arranged in 15 storeys, on the ground floor, plus a basement for mechanical installations and car-parks. The programme includes: 704 double rooms; 111 single rooms; 8 suites; 95 rooms for hotel staff; restaurant facilities; bar and cafeteria; three conference rooms; a public gallery for different uses; kitchens, several floors for mechanical installations; underground garage; canteen and rooms for staff, and management offices.

    Ha sido construido a orillas del Támesis, y debe su nombre a la proximidad de la Torre de Londres, que igualmente nominó al antiguo Tower Bridge, puente sobre el río, situado a escasos metros del edificio. La fachada posterior del hotel se orienta hacia la inmediata Dársena de Santa Catalina, completando un entorno atractivo en una de las zonas paradójicamente peor aprovechadas de la ciudad. La construcción ocupa una edificabilidad total de 36.560 m2 organizados en 15 plantas, sobre rasante, más un sótano dedicado a las instalaciones mecánicas y a aparcamientos. El programa comprende: 704 habitaciones dobles; 111 individuales; 8 suites; 95 habitaciones para el personal del hotel; servicios de restaurante; bar y cafetería; tres salas de conferencias; una galería pública de funcionamiento diverso; cocinas; varias plantas mecánicas de instalaciones; garaje subterráneo; comedor y salas para el personal, y oficinas de administración.

  7. Pääsküla raamatukogu / Maris Takk

    Index Scriptorium Estoniae

    Takk, Maris

    2006-01-01

    Pääsküla raamatukogu (Tallinn, Pärnu mnt. 480A) sisekujundus, mille eest sisekujundajad Katrin Kaevats ja Peeter Pere (AB Muru & Pere) pälvisid Eesti Sisearhitektide Liidu 2005. a. ühiskondliku interjööri preemia. Ehitaja: OÜ SCE Grupp. K. Kaevatsist, tema tähtsamad tööd. P. Perest, valik AB Muru & Pere projektidest. Ill.: kaks korruste plaani, 17 värv. vaadet, K. Kaevatsi ja P. Pere fotod

  8. Pääsküla raamatukogu / Maris Takk

    Index Scriptorium Estoniae

    Takk, Maris

    2006-01-01

    Pääsküla raamatukogu (Tallinn, Pärnu mnt. 480A) sisekujundus, mille eest sisekujundajad Katrin Kaevats ja Peeter Pere (AB Muru & Pere) pälvisid Eesti Sisearhitektide Liidu 2005. a. ühiskondliku interjööri preemia. Ehitaja: OÜ SCE Grupp. K. Kaevatsist, tema tähtsamad tööd. P. Perest, valik AB Muru & Pere projektidest. Ill.: kaks korruste plaani, 17 värv. vaadet, K. Kaevatsi ja P. Pere fotod

  9. CP Violation in $D^0\\rightarrow K_SK_S$

    CERN Document Server

    Nierste, Ulrich

    2015-01-01

    The direct CP asymmetry $a_{CP}^{\\mathrm{dir}}(D^0\\rightarrow K_SK_S)$ involves exchange diagrams which are induced at tree-level in the Standard Model. Since the corresponding topological amplitude $E_{KK}$ can be large, $D^0\\rightarrow K_SK_S$ is a promising discovery channel for charm CP violation. We estimate the penguin annihilation amplitude with a perturbative calculation and extract the exchange amplitude $E_{KK}$ from a global fit to $D$ branching ratios. Our results are further used to predict the size of mixing-induced CP violation. We obtain $\\vert a_{CP}^{\\mathrm{dir}}(D^0\\rightarrow K_SK_S)\\vert \\leq 1.1\\%$ (95\\% C.L.). The same bound applies to the nonuniversal part of the phase between the $D\\!-\\overline{\\!D}\\,$ mixing and decay amplitudes. If future data exceed our predictions, this will point to new physics or an enhancement of the penguin annihilation amplitude by QCD dynamics. We briefly discuss the implications of these possibilities for other CP asymmetries.

  10. Claves para el conocimiento de la configuración espacial de una torre banderiza: la Torre de Murga (siglos XIV-XV

    Directory of Open Access Journals (Sweden)

    García Gómez, Ismael

    2003-12-01

    Full Text Available This work schematically presents the results of archaeological analysis of the torre banderiza (a type of tower particular to the Basque Country of Murga (Ayala, Álava and makes use of these —in combination with results found at similar towers— to establish a proposal of interpretation of the spatial basis of this type of tower. The work highlights, among other aspects, the importance of the platform/ scaffold and the inexistence of ground floor access in this type of construction. Lastly the work summarises the criteria which, in the authors’ opinion, should be followed in analysing a tower of these characteristics.En este trabajo se presentan esquemáticamente los resultados del análisis arqueológico de la torre banderiza de Murga (Ayala, Álava, y se hace uso de ellos —en combinación con los resultados arrojados por otras torres similares— para establecer una propuesta de interpretación de la articulación espacial de este tipo de edificaciones. Entre otras cosas se resalta la importancia del cadalso y la inexistencia de un acceso por la planta baja. Finalmente se reseñan los criterios que, a juicio de los autores, deben ser seguidos a la hora de analizar una torre de estas características.

  11. Mitochondrial small conductance SK2 channels prevent glutamate-induced oxytosis and mitochondrial dysfunction.

    Science.gov (United States)

    Dolga, Amalia M; Netter, Michael F; Perocchi, Fabiana; Doti, Nunzianna; Meissner, Lilja; Tobaben, Svenja; Grohm, Julia; Zischka, Hans; Plesnila, Nikolaus; Decher, Niels; Culmsee, Carsten

    2013-04-12

    Small conductance calcium-activated potassium (SK2/K(Ca)2.2) channels are known to be located in the neuronal plasma membrane where they provide feedback control of NMDA receptor activity. Here, we provide evidence that SK2 channels are also located in the inner mitochondrial membrane of neuronal mitochondria. Patch clamp recordings in isolated mitoplasts suggest insertion into the inner mitochondrial membrane with the C and N termini facing the intermembrane space. Activation of SK channels increased mitochondrial K(+) currents, whereas channel inhibition attenuated these currents. In a model of glutamate toxicity, activation of SK2 channels attenuated the loss of the mitochondrial transmembrane potential, blocked mitochondrial fission, prevented the release of proapoptotic mitochondrial proteins, and reduced cell death. Neuroprotection was blocked by specific SK2 inhibitory peptides and siRNA targeting SK2 channels. Activation of mitochondrial SK2 channels may therefore represent promising targets for neuroprotective strategies in conditions of mitochondrial dysfunction.

  12. Mitochondrial Small Conductance SK2 Channels Prevent Glutamate-induced Oxytosis and Mitochondrial Dysfunction*

    Science.gov (United States)

    Dolga, Amalia M.; Netter, Michael F.; Perocchi, Fabiana; Doti, Nunzianna; Meissner, Lilja; Tobaben, Svenja; Grohm, Julia; Zischka, Hans; Plesnila, Nikolaus; Decher, Niels; Culmsee, Carsten

    2013-01-01

    Small conductance calcium-activated potassium (SK2/KCa2.2) channels are known to be located in the neuronal plasma membrane where they provide feedback control of NMDA receptor activity. Here, we provide evidence that SK2 channels are also located in the inner mitochondrial membrane of neuronal mitochondria. Patch clamp recordings in isolated mitoplasts suggest insertion into the inner mitochondrial membrane with the C and N termini facing the intermembrane space. Activation of SK channels increased mitochondrial K+ currents, whereas channel inhibition attenuated these currents. In a model of glutamate toxicity, activation of SK2 channels attenuated the loss of the mitochondrial transmembrane potential, blocked mitochondrial fission, prevented the release of proapoptotic mitochondrial proteins, and reduced cell death. Neuroprotection was blocked by specific SK2 inhibitory peptides and siRNA targeting SK2 channels. Activation of mitochondrial SK2 channels may therefore represent promising targets for neuroprotective strategies in conditions of mitochondrial dysfunction. PMID:23430260

  13. Sensitivity analysis of the physical dynamics of the Fly River plume in Torres Strait

    Science.gov (United States)

    Li, Yanfang; Martins, Flavio; Wolanski, Eric

    2017-07-01

    The intrusion in the Torres Strait of the Fly River plume polluted by mine tailings is an international water issue. To investigate the physical mechanisms involved in the intrusion process, numerical simulations were conducted to assess the relative influence of the bathymetry and the external forcing, namely the tides, the mean sea level slope across the strait, river runoff and wind forcing. The wind data from Horn Island, the only long-term wind station in the Torres Strait, is shown to be unreliable due to orographic effects. Mean sea level data from altimetry compare well with those from tide gauges in these shallow, reef-studded waters. The wind has a dominant effect on the mean sea level at seasonal and inter-annual periods. The resulting mean sea level difference across the strait fluctuated seasonally and strongly influenced the intrusion of the Fly River plume in the Torres Strait. The 3D finite-volume MOHID model reproduced the observation that the river plume starts by being stratified in the Gulf of Papua where it originates, and it mixes vertically when it enters the Torres Strait. The MOHID and the SLIM models were applied with different resolution to the Torres Strait and responded similarly to the external forcings. The predicted and observed Fly River plume intrusion in the eastern Torres Strait agreed well with each other, including the formation of patches due to flow reversals. However, the two models predicted a widely different Fly River plume in its far field in the western Torres Strait, the differences were attributed to the different bathymetry in the Australian and British-US bathymetry data for these poorly charted waters, which demonstrated the importance of the details of the bathymetry in controlling the extent of plume intrusion.

  14. New deflected-beam gauge for pressures below 10/sup -12/ Torr

    CERN Document Server

    Blechschmidt, Diether

    1975-01-01

    A new ionization gauge for measurement of total gas pressures between 10/sup -12/ torr and 10/sup -14/ torr is described. The gauge's main features are a pair of collimating slots below the grid structure, two concentric hemi-spherical deflecting electrodes and a channel electron multiplier which detects low-energy ions passing through the deflector system. The detection of low-energy ions allows discrimination against desorbed ions (which are more energetic), while spurious signals due to X-rays may also be rejected. The channel electron multiplier leads to gauge sensitivities which are much higher than those of ordinary ionization gauges. The gauge has been tested down to 4*10/sup -13/ torr and found to have sensitivities of 1.5*10/sup 17/ and 8*10/sup 16 / counts/s.A.torr for N/sub 2/ and H/sub 2/ respectively. For a channel electron multiplier gain of 10/sup 7/ the gauge sensitivity is thus approximately=10/sup 5//torr. (17 refs).

  15. Effective communication tools to engage Torres Strait Islanders in scientific research

    Science.gov (United States)

    Jones, A.; Barnett, B.; Williams, A. J.; Grayson, J.; Busilacchi, S.; Duckworth, A.; Evans-Illidge, E.; Begg, G. A.; Murchie, C. D.

    2008-09-01

    Often, research activities in Torres Strait have not delivered full benefit to Torres Strait Islanders due to a lack of consultation, ineffectual communication of research information and lack of empathy for the needs of Islander communities. As for other stakeholder groups, integration of Islanders into the research process through practical involvement in research may overcome these problems. Three case studies from research projects conducted in Torres Strait are discussed to highlight a variety of communication and engagement activities carried out by non-Indigenous researchers. How these communication and extension activities facilitate collaboration between Torres Strait Islander and non-Indigenous researchers provides insight in the importance of these activities to the relative success of research projects. The benefits for Islanders in collaborating with researchers may be: improved understanding of the research and how it contributes to natural resource management; a sense of control in future management decisions; a greater likelihood of successful self-regulatory management systems; enhanced skills; and increased employment opportunities. The potential benefits for researchers are enhanced support for research projects resulting in increased access to data and logistic support that may ultimately impact the successful completion of projects. Such an approach will require researchers to take time to develop relationships with Torres Strait Islanders, effectively involve Islanders in research on an equitable basis and be flexible. This will ultimately require funding organisations to recognise the importance of such activities in research proposals and provide support through sufficient funding to enable these activities to be carried out.

  16. Australia's Efforts to Improve Food Security for Aboriginal and Torres Strait Islander Peoples.

    Science.gov (United States)

    Davy, Deanna

    2016-12-01

    Australia is a wealthy country; however, available evidence suggests that food security among Aboriginal and Torres Strait Islander peoples has not yet been achieved. Aboriginal and Torres Strait Islander peoples living in remote, regional, and urban parts of Australia experience food insecurity for a number of reasons that usually include low income and a lack of access to affordable and healthy food. The much higher rate of illness and disease that this population experiences compared to non-indigenous Australians is directly related to food insecurity. This paper examines the food insecurity among Aboriginal and Torres Strait Islander peoples and recent Australian government efforts to combat this problem. The paper first considers what constitutes a human rights-based approach to achieving food security. Second, it describes the food insecurity that currently exists among Aboriginal and Torres Strait Islander peoples across the three pillars of food access, food availability, and food use. Third, the paper critically examines recent and current Australian government policy aimed at improving food security. The paper concludes with some reflections regarding how the Australian government can improve its efforts to achieve food security for Aboriginal and Torres Strait Islander peoples.

  17. Solar Neutrino flare detection in Hyperkamiokande and SK

    Science.gov (United States)

    Fargion, Daniele

    2016-07-01

    The possible buid and near activity of a Megaton neutrino detection in HyperKamiokande and the older SK implementation by Gadolinium liqid might open to future detection of largest solar flare (pion trace at tens MeV) electron neutrino and antineutrino. The multiwave detection of X-gamma and neutrino event might offer a deep view of such solar acelleration and of neutrino flavor mix along its flight. The possoble near future discover of such events will open a third neutrino astronomy windows after rarest SN 1987A and persistent Solar nuclear signals.

  18. Hepatoma SK Hep-1 cells exhibit characteristics of oncogenic mesenchymal stem cells with highly metastatic capacity.

    Directory of Open Access Journals (Sweden)

    Jong Ryeol Eun

    Full Text Available BACKGROUND: SK Hep-1 cells (SK cells derived from a patient with liver adenocarcinoma have been considered a human hepatoma cell line with mesenchymal origin characteristics, however, SK cells do not express liver genes and exhibit liver function, thus, we hypothesized whether mesenchymal cells might contribute to human liver primary cancers. Here, we characterized SK cells and its tumourigenicity. METHODS AND PRINCIPAL FINDINGS: We found that classical mesenchymal stem cell (MSC markers were presented on SK cells, but endothelial marker CD31, hematopoietic markers CD34 and CD45 were negative. SK cells are capable of differentiate into adipocytes and osteoblasts as adipose-derived MSC (Ad-MSC and bone marrow-derived MSC (BM-MSC do. Importantly, a single SK cell exhibited a substantial tumourigenicity and metastatic capacity in immunodefficient mice. Metastasis not only occurred in circulating organs such as lung, liver, and kidneys, but also in muscle, outer abdomen, and skin. SK cells presented greater in vitro invasive capacity than those of Ad-MSC and BM-MSC. The xenograft cells from subcutaneous and metastatic tumors exhibited a similar tumourigenicity and metastatic capacity, and showed the same relatively homogenous population with MSC characteristics when compared to parental SK cells. SK cells could unlimitedly expand in vitro without losing MSC characteristics, its tumuorigenicity and metastatic capacity, indicating that SK cells are oncogenic MSC with enhanced self-renewal capacity. We believe that this is the first report that human MSC appear to be transformed into cancer stem cells (CSC, and that their derivatives also function as CSCs. CONCLUSION: Our findings demonstrate that SK cells represent a transformation mechanism of normal MSC into an enhanced self-renewal CSC with metastasis capacity, SK cells and their xenografts represent a same relative homogeneity of CSC with substantial metastatic capacity. Thus, it represents a

  19. Adenoma sebáceo en un paciente con síndrome de Muir-Torre Sebaceous adenoma in a patient with a Muir-Torre syndrome

    Directory of Open Access Journals (Sweden)

    E Matamoros

    2012-06-01

    Full Text Available El síndrome de Muir-Torre comprende la asociación de uno o más tumores de glándulas sebáceas, con una o más malignidades internas y antecedentes familiares de neoplasias. Presentamos el caso de un paciente masculino de 33 años, con antecedentes familiares de cáncer de colon, que consulta a nuestro servicio por lesión en cuero cabelludo de seis meses de evolución, con posterior diagnóstico histopatológico de adenoma sebáceo. Dentro de los exámenes complementarios se observan por colonoscopía, dos lesiones que correspondieron a adenocarcinomas de colon bien diferenciados. Con los antecedentes familiares, el examen clínico y la histopatología se efectúa el diagnóstico de síndrome de Muir-Torre.Muir-Torre syndrome represents the association of one or more sebaceous tumors, with one or more visceral malignancies and a family history of cancer. We report the case of a 33-year-old man, with a family history of colon cancer, who presented with a scalp tumor. The histopathological diagnosis was sebaceous adenoma. Two lesions were found in the colonoscopy, which corresponded to colon adenocarcionomas. With a compatible personal and family history of cancer and the results of the histopathology, we diagnosed the patient as Muir-Torre syndrome.

  20. Ramón Torres Méndez y la pintura de tipos y costumbres

    Directory of Open Access Journals (Sweden)

    Efraín Sánchez Cabra

    1991-09-01

    Full Text Available En 1940, al anunciar la publicación de 37 "escenas de costumbres" de Ramón Torres Méndez, la revista Cromos lo presentó como "el pintor más famoso de Colombia". A juzgar por casi un siglo de testimonios, Cromos no exageraba. Hasta mediados del siglo XX las "escenas de costumbres" de Torres Méndez fueron uno de los recursos más frecuentemente utilizados en los periódicos y revistas locales para la ilustración de relatos de viajes, descripciones de tradiciones y costumbres bogotanas y aun para la publicidad de industrias y casas comerciales. Hasta mediados del presente siglo, los escasos críticos y comentaristas del arte nacional fueron unánimes en situar a Torres Méndez como el pintor más notable de la Nueva Granada en el siglo XIX.

  1. Longitudinal epitope mapping in MuSK myasthenia gravis: implications for disease severity.

    Science.gov (United States)

    Huijbers, Maartje G; Vink, Anna-Fleur D; Niks, Erik H; Westhuis, Ruben H; van Zwet, Erik W; de Meel, Robert H; Rojas-García, Ricardo; Díaz-Manera, Jordi; Kuks, Jan B; Klooster, Rinse; Straasheijm, Kirsten; Evoli, Amelia; Illa, Isabel; van der Maarel, Silvère M; Verschuuren, Jan J

    2016-02-15

    Muscle weakness in MuSK myasthenia gravis (MG) is caused predominantly by IgG4 antibodies which block MuSK signalling and destabilize neuromuscular junctions. We determined whether the binding pattern of MuSK IgG4 antibodies change throughout the disease course ("epitope spreading"), and affect disease severity or treatment responsiveness. We mapped the MuSK epitopes of 255 longitudinal serum samples of 53 unique MuSK MG patients from three independent cohorts with ELISA. Antibodies against the MuSK Iglike-1 domain determine disease severity. Epitope spreading outside this domain did not contribute to disease severity nor to pyridostigmine responsiveness. This provides a rationale for epitope specific treatment strategies.

  2. Entrevista a Rafael Martínez Raymonda, sobre la figura de Lisandro de la Torre

    OpenAIRE

    Martínez Raymonda, Rafael; Toschi, Damián

    2010-01-01

    Rafael Martínez Raymonda, secretario general del partido Demócrata Progresista, es entrevistado por Damián Toschi para el programa "Derecho de autor" con motivo de los 142 años del nacimiento de Lisandro de la Torre, fundador del partido y político fundamental del siglo XX. Lisandro de la Torre: Nació el 6 de diciembre de 1868 en Santa Fe y se suicidó el 5 de enero de 1939 en Buenos Aires.

  3. Subtype-specific, bi-component inhibition of SK channels by low internal pH

    DEFF Research Database (Denmark)

    Peitersen, Torben; Jespersen, Thomas; Jorgensen, Nanna K;

    2006-01-01

    The effects of low intracellular pH (pH(i) 6.4) on cloned small-conductance Ca2+-activated K+ channel currents of all three subtypes (SK1, SK2, and SK3) were investigated in HEK293 cells using the patch-clamp technique. In 400 nM internal Ca2+ [Ca2+]i, all subtypes were inhibited by pH(i) 6...

  4. Characterization of CD4 and CD8 T Cell Responses in MuSK Myasthenia Gravis

    OpenAIRE

    Yi, JS; Guidon, A; Sparks, S.; Osborne, R.; Juel, VC; Massey, JM; Sanders, DB; Weinhold, KJ; Guptill, JT

    2013-01-01

    Muscle specific tyrosine kinase myasthenia gravis (MuSK MG) is a form of autoimmune MG that predominantly affects women and has unique clinical features, including prominent bulbar weakness, muscle atrophy, and excellent response to therapeutic plasma exchange. Patients with MuSK MG have predominantly IgG4 autoantibodies directed against MuSK on the postsynaptic muscle membrane. Lymphocyte functionality has not been reported in this condition. The goal of this study was to characterize T-cell...

  5. File list: ALL.Brs.10.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Brs.10.AllAg.SK-BR-3 hg19 All antigens Breast SK-BR-3 SRX155755,SRX750670,SRX75...0675,SRX750660,SRX750665,SRX155756,SRX128102,SRX155754,SRX469109,SRX469108,SRX469107 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.Brs.10.AllAg.SK-BR-3.bed ...

  6. File list: ALL.Brs.50.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Brs.50.AllAg.SK-BR-3 hg19 All antigens Breast SK-BR-3 SRX750670,SRX750665,SRX12...8102,SRX750660,SRX750675,SRX469109,SRX469107,SRX469108,SRX155755,SRX155756,SRX155754 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.Brs.50.AllAg.SK-BR-3.bed ...

  7. File list: ALL.Brs.20.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Brs.20.AllAg.SK-BR-3 hg19 All antigens Breast SK-BR-3 SRX750670,SRX750665,SRX75...0675,SRX155755,SRX155756,SRX128102,SRX750660,SRX155754,SRX469109,SRX469107,SRX469108 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.Brs.20.AllAg.SK-BR-3.bed ...

  8. File list: ALL.Brs.05.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Brs.05.AllAg.SK-BR-3 hg19 All antigens Breast SK-BR-3 SRX155754,SRX155755,SRX75...0670,SRX750660,SRX750675,SRX750665,SRX155756,SRX128102,SRX469109,SRX469108,SRX469107 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/ALL.Brs.05.AllAg.SK-BR-3.bed ...

  9. Indigenous Language Learning and Maintenance among Young Australian Aboriginal and Torres Strait Islander Children

    Science.gov (United States)

    Verdon, Sarah; McLeod, Sharynne

    2015-01-01

    Internationally, cultural renewal and language revitalisation are occurring among Indigenous people whose lands were colonised by foreign nations. In Australia, the Aboriginal and Torres Strait Islander people are striving for the re-voicing of their mother tongue and the re-practicing of their mother culture to achieve cultural renewal in the…

  10. 25 CFR 162.502 - Cabazon, Augustine, and Torres-Martinez Reservations, California.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Cabazon, Augustine, and Torres-Martinez Reservations... WATER LEASES AND PERMITS Special Requirements for Certain Reservations § 162.502 Cabazon, Augustine, and... owners are not making beneficial use thereof, restricted lands on the Cabazon, Augustine, and...

  11. Social Exclusion/Inclusion for Urban Aboriginal and Torres Strait Islander People

    Directory of Open Access Journals (Sweden)

    Maggie Walter

    2016-02-01

    Full Text Available Social exclusion social inclusion are useful concepts for making sense of the deeply embedded socio-economic disadvantaged position of Aboriginal and Torres Islander people in Australian. The concepts not only describe exclusion from social and economic participation; but seek to understand the dynamic processes behind their creation and reproduction. Yet few Australian studies go beyond describing Aboriginal over-representation on social exclusion indicators. Neither do they address the translatability of the concepts from non-Indigenous to Indigenous contexts despite mainstream studies finding the pattern of social exclusion (and therefore what social inclusion might look like differs for Aboriginal and Torres Strait Islander people to that of other disadvantaged groups. This paper uses data from the Longitudinal Study of Indigenous children to explore patterns of social exclusion across social, economic, well-being and community dimensions for urban Aboriginal and Torres Strait families. The paper then develops a contextual understanding of the processes and patterns that create and sustain social exclusion and the opportunities and challenges of moving to greater social inclusion for urban Aboriginal and Torres Strait Islander people/s.

  12. Indigenous Language Learning and Maintenance among Young Australian Aboriginal and Torres Strait Islander Children

    Science.gov (United States)

    Verdon, Sarah; McLeod, Sharynne

    2015-01-01

    Internationally, cultural renewal and language revitalisation are occurring among Indigenous people whose lands were colonised by foreign nations. In Australia, the Aboriginal and Torres Strait Islander people are striving for the re-voicing of their mother tongue and the re-practicing of their mother culture to achieve cultural renewal in the…

  13. Propiedades químicas, estructurales y funcionales de la lechuguilla (Agave lechuguilla Torr.)

    DEFF Research Database (Denmark)

    Carmona, Juan E.; Morales-Martínez, Thelma K.; Mussatto, Solange I.

    2017-01-01

    Agave lechuguilla Torr. Is a non-timber forest resource in the arid and semi-arid zones of northeastern Mexico, due to its range of distribution, it is interesting to evaluate the potential of this raw material for biotechnological applications such as the production of second generation biofuels...

  14. CyPPA, a positive SK3/SK2 modulator, reduces activity of dopaminergic neurons, inhibits dopamine release, and counteracts hyperdopaminergic behaviors induced by methylphenidate

    Directory of Open Access Journals (Sweden)

    Kjartan F. Herrik

    2012-02-01

    Full Text Available Dopamine (DA containing midbrain neurons play critical roles in several psychiatric and neurological diseases, including schizophrenia and attention deficit hyperactivity disorder (ADHD, and the substantia nigra pars compacta neurons selectively degenerate in Parkinson’s disease. Pharmacological modulation of DA receptors and transporters are well established approaches for treatment of DA-related disorders. Direct modulation of the DA system by influencing the discharge pattern of these autonomously firing neurons has yet to be exploited as a potential therapeutic strategy. Small conductance Ca2+-activated K+ channels (SK channels, in particular the SK3 subtype, are important in the physiology of DA neurons, and agents modifying SK channel activity could potentially affect DA-signaling and DA-related behaviors. Here we show that CyPPA (cyclohexyl-[2-(3,5-dimethyl-pyrazol-1-yl-6-methyl-pyrimidin-4-yl]-amine, a subtype-selective positive modulator of SK channels (SK3 > SK2 >>> SK1, IK, decreased spontaneous firing rate, increased the duration of the apamin-sensitive, medium duration afterhyperpolarization (mAHP, and caused an activity-dependent inhibition of current-evoked action potentials in DA neurons from both mouse and rat midbrain slices. Using a immunohistochemically and pharmacologically validated DA release assay employing cultured DA neurons from rats, we show that CyPPA repressed DA release in a concentration-dependent manner with a maximal effect equal to the D2 receptor agonist quinpirole. In vivo studies revealed that systemic administration of CyPPA attenuated methylphenidate-induced hyperactivity and stereotypic behaviors in mice. Taken together, the data accentuate the important role played by SK3 channels in the physiology of DA neurons, and indicate that their facilitation by CyPPA profoundly influences physiological as well as pharmacologically induced hyperdopaminergic behavior.

  15. Introduced marine species: Management arrangements of consideration for the Torres Strait

    Science.gov (United States)

    Neil, Kerry M.; Hilliard, Robert; Russell, Barry; Clark, Paul

    2008-09-01

    Introduced marine species (IMS) can have significant impacts on economic, ecological, social and cultural aspects of coastal marine environments. There is, therefore, a need to minimise these impacts through the implementation of comprehensive and consistent management strategies and monitoring processes that work towards preventing introductions, detecting introductions if they were to occur and managing incursions should they be detected. There is also the need to ensure that approaches taken are comparable to provide consistency of IMS management effort, particularly across areas that are multi-jurisdictional (e.g. spanning State or Territory borders). A number of studies have examined IMS, their vectors and management arrangements for the Torres Strait region, a multi-jurisdictional area between Queensland, Australia and Papua New Guinea. This paper presents information from a baseline survey conducted in the Torres Strait for marine pests in conjunction with a synthesis of information from other studies on IMS risks, their vectors and management strategies of relevance to the Torres Strait region. A number of strengths, but also gaps, between the various jurisdictions have been identified and discussed. Processes such as the establishment of Biosecurity Queensland as a specialist group and the development of the National System for the Prevention and Management of Marine Pest Incursions are occurring. However, the Torres Strait region is at current risk of marine pest inoculation, particularly for species such as Perna viridis, and management strategies to minimise this risk are lacking. The information presented here will assist management groups in their ability to work towards mitigating the risk of an IMS being translocated to and negatively impacting the unique and diverse ecology of the marine systems, and the industries, people and customs that rely on the coastal marine environment for food, recreation and commerce in the Torres Strait.

  16. The Poetics of Trauma and Hope in Wounded Water by Anabel Torres

    Directory of Open Access Journals (Sweden)

    Cecilia Castro Lee

    2011-06-01

    Full Text Available Wounded Water / Agua herida (Bogotá 2004 es un libro de poesía bilingüe de la poeta colombiana contemporánea Anabel Torres (1948. Esta obra ofrece un encuentro poético con el trauma o “las pequeñas sacudidas personales” de las que consistía, en esencia, la poesía de Charles Baudelaire para Paul Valery. Torres escribe de su vida cotidiana como mujer, como poeta y como emigrante radicada en Europa durante las últimas dos décadas. Propongo que Wounded Water /Agua herida es poesía de supervivencia, similar a la que Gregory Orr define como “poesía lírica de transformación”. El campo semántico del agua en la poesía de Torres conceptualiza un amplio espectro de ciclos vitales.Wounded Water / Agua herida (Bogotá 2004 is a bilingual book of poetry by contemporary Colombian poet Anabel Torres (1948. This book offers a poetic encounter with the trauma or “minute personal shocks» Paul Valery cites when referring to Baudelaire’s poetry. Torres writes of her everyday existence as a woman, as a poet, and as an emigrant living in Europe for the last two decades or more. I contend that Wounded Water/ Agua herida is poetry of survival, similar to what Gregory Orr defines as “transformational lyric poetry”. The semantic field of water in Torres’s poetry conceptualizes a broad spectrum from birth, death, and rebirth.

  17. La Torre de Augusto en la Campa Torres (Gijón, Asturias. Las antiguas excavaciones y el epígrafe de Calpurnio Pisón

    Directory of Open Access Journals (Sweden)

    Fernández Ochoa, Carmen

    2005-12-01

    Full Text Available Through the historiographic data and the architectonic and archaeologic study of the Roman remains found during the 18th and 19th Centuries, today disappeared, we present here an hypothesis about the Augustean construction of a big tower in Campa Torres. This would be identified as a lighthouse controlling the entrance of the Gijón's bay. The commemorative stone of Cn. Calpurnius Piso (CIL II, 2703, dated on 9-10 AD, was founded in this building. We cannot accept the identification of this monument as one of the wellknown Arae Sestianae. This light-house, with a symbolic character as well a purpose of informing to the mariners the access of the harbour, would be erected in a militarized region recently conquered.Partiendo del análisis exhaustivo de la documentación historiográfica y del estudio arqueoarquitectónico de los restos arqueológicos exhumados en los siglos XVIII y XIX, hoy en día destruidos, planteamos la hipótesis de que en la Campa Torres se erigió en época augustea una gran torre con función de faro, que señalaba el acceso a la bahía de Gijón. Dicho monumento, del que procede la lapida calpurniana datada en el 9-10 d. C. (CIL II, 2703, no puede de ninguna manera interpretarse como una de las famosas Aras Sestianas. Con un carácter claramente simbólico, el faro constituiría un hito señalizador en el paisaje de una región militarizada y recientemente conquistada, además de desempeñar un claro papel de apoyo a la navegación cantábrica, al indicar la entrada al mejor puerto astur.

  18. La Torre de Augusto en la Campa Torres (Gijón, Asturias). Las antiguas excavaciones y el epígrafe de Calpurnio Pisón

    OpenAIRE

    Fernández Ochoa, Carmen; Morillo Cerdán, Ángel; Villa Valdés, Ángel

    2005-01-01

    Through the historiographic data and the architectonic and archaeologic study of the Roman remains found during the 18th and 19th Centuries, today disappeared, we present here an hypothesis about the Augustean construction of a big tower in Campa Torres. This would be identified as a lighthouse controlling the entrance of the Gijón's bay. The commemorative stone of Cn. Calpurnius Piso (CIL II, 2703), dated on 9-10 AD, was founded in this building. We cannot accept the identification of this m...

  19. Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice.

    Science.gov (United States)

    Demyanenko, Ilya A; Popova, Ekaterina N; Zakharova, Vlada V; Ilyinskaya, Olga P; Vasilieva, Tamara V; Romashchenko, Valeria P; Fedorov, Artem V; Manskikh, Vasily N; Skulachev, Maxim V; Zinovkin, Roman A; Pletjushkina, Olga Yu; Skulachev, Vladimir P; Chernyak, Boris V

    2015-07-01

    The process of skin wound healing is delayed or impaired in aging animals. To investigate the possible role of mitochondrial reactive oxygen species (mtROS) in cutaneous wound healing of aged mice, we have applied the mitochondria-targeted antioxidant SkQ1. The SkQ1 treatment resulted in accelerated resolution of the inflammatory phase, formation of granulation tissue, vascularization and epithelization of the wounds. The wounds of SkQ1-treated mice contained increased amount of myofibroblasts which produce extracellular matrix proteins and growth factors mediating granulation tissue formation. This effect resembled SkQ1-induced differentiation of fibroblasts to myofibroblast, observed earlierin vitro. The Transforming Growth Factor beta (TGFb) produced by SkQ1-treated fibroblasts was found to stimulated motility of endothelial cells in vitro, an effect which may underlie pro-angiogenic action of SkQ1 in the wounds. In vitro experiments showed that SkQ1 prevented decomposition of VE-cadherin containing contacts and following increase in permeability of endothelial cells monolayer, induced by pro-inflammatory cytokine TNF. Prevention of excessive reaction of endothelium to the pro-inflammatory cytokine(s) might account for anti-inflammatory effect of SkQ1. Our findings point to an important role of mtROS in pathogenesis of age-related chronic wounds.

  20. SK2 channels regulate mitochondrial respiration and mitochondrial Ca2+ uptake

    NARCIS (Netherlands)

    Honrath, Birgit; Matschke, Lina; Meyer, Tammo; Magerhans, Lena; Perocchi, Fabiana; Ganjam, Goutham K; Zischka, Hans; Krasel, Cornelius; Gerding, Albert; Bakker, Barbara M; Bünemann, Moritz; Strack, Stefan; Decher, Niels; Culmsee, Carsten; Dolga, Amalia M

    Mitochondrial calcium ([Ca(2+)]m) overload and changes in mitochondrial metabolism are key players in neuronal death. Small conductance calcium-activated potassium (SK) channels provide protection in different paradigms of neuronal cell death. Recently, SK channels were identified at the inner

  1. Skønhedens metamorfose - De æstetiske idéers historie

    DEFF Research Database (Denmark)

    Jørgensen, Dorthe

    Hvorfor findes der kunst? Hvad vil guderne os? Hvordan forholder kunst, skønhed og guddommelighed sig til hinanden? Disse spørgsmål er blevet diskuteret siden antikken. Og lige så tit der er blevet formuleret nye svar, lige så ofte er skønhedsopfattelsen undergået en metamorfose. Det er dog gammel...

  2. Effects of the mitochondria-targeted antioxidant SkQ1 on lifespan of rodents

    Science.gov (United States)

    Anisimov, Vladimir N.; Egorov, Maxim V.; Krasilshchikova, Marina S.; Lyamzaev, Konstantin G.; Manskikh, Vasily N.; Moshkin, Mikhail P.; Novikov, Evgeny A.; Popovich, Irina G.; Rogovin, Konstantin A.; Shabalina, Irina G.; Shekarova, Olga N.; Skulachev, Maxim V.; Titova, Tatiana V.; Vygodin, Vladimir A.; Vyssokikh, Mikhail Yu.; Yurova, Maria N.; Zabezhinsky, Mark A.; Skulachev, Vladimir P.

    2011-01-01

    The effect of the mitochondria-targeted, plastoquinone-containing antioxidant SkQ1 on the lifespan of outbred mice and of three strains of inbred mice was studied. To this end, low pathogen (LP) or specific pathogen free (SPF) vivaria in St. Petersburg, Moscow, and Stockholm were used. For comparison, we also studied mole-voles and dwarf hamsters, two wild species of small rodents kept under simulated natural conditions. It was found that substitution of a LP vivarium for a conventional (non-LP) one doubled the lifespan of female outbred mice, just as SkQ1 did in a non-LP vivarium. SkQ1 prevented age-dependent disappearance of estrous cycles of outbred mice in both LP and non-LP vivaria. In the SPF vivarium in Moscow, male BALB/c mice had shorter lifespan than females, and SkQ1 increased their lifespan to the values of the females. In the females, SkQ1 retarded development of such trait of aging as heart mass increase. Male C57Bl/6 mice housed individually in the SPF vivarium in Stockholm lived as long as females. SkQ1 increased the male lifespan, the longevity of the females being unchanged. SkQ1 did not change food intake by these mice. Dwarf hamsters and mole-voles kept in outdoor cages or under simulated natural conditions lived longer if treated with SkQ1. The effect of SkQ1 on longevity of females is assumed to mainly be due to retardation of the age-linked decline of the immune system. For males under LP or SPF conditions, SkQ1 increased the lifespan, affecting also some other system(s) responsible for aging. PMID:22166671

  3. SkTech:俄罗斯工程教育的模式创新%SkTech: An Innovative Model of Engineering Education in Russia

    Institute of Scientific and Technical Information of China (English)

    孔寒冰; 吴婧姗; 李文

    2012-01-01

    The Skolkovo Institute of Science and Technology(SkTech) is a new Russian research university in an international context built in Skolkovo recently.With the participation and support of MIT,it provides graduate programs in science and engineering.This paper briefly describes profound context of SkTech,Innovation Entrepreneurship(IE) ideas and practice,novel organization and culture,and the unique development model as well.It is worthwhile to notice that SkTech innovation mode is a triple helix of integrating Research-Education-IE.This also represents the important essence of SkTech innovative model and its key role in Skolkovo innovation ecosystem.%俄罗斯的SkTech(斯科尔科沃理工学院)是在MIT的鼎力支持下新近创办的一所理工科研究生大学。本文简要介绍它的建校背景、先进的办学理念、新颖的组织架构与文化,以及独特的办学模式与实践。SkTech创新模式的精彩之处,在于它的"研究、教育、创业创新"三重螺旋集成的协同创新。这也是Sk-Tech将在斯科尔科沃创新生态系统中发挥关键作用的重要机制。

  4. Invasive pneumococcal disease in New South Wales, Australia: reporting Aboriginal and Torres Strait Islander status improves epidemiology

    Directory of Open Access Journals (Sweden)

    David N Durrheim

    2011-07-01

    Full Text Available The aim of this work was to determine the feasibility of improving Aboriginal and Torres Strait Islander status recording for notifiable diseases using all Invasive Pneumococcal Disease (IPD notifications in a regional area of New South Wales, Australia.In Australia people with IPD are nearly always admitted to hospital and their Aboriginal and Torres Strait Islander status is recorded. Aboriginal and Torres Strait Islander status was determined for IPD notifications by referring to the routine hospital admission data, in a regional area of New South Wales, Australia.There were 234 notifications in the regional area of Hunter New England during the period 2007–2009. Initially, 168 (72% notifications had Aboriginal and Torres Strait Islander status recorded. After referring to the routine hospital admission data the recorded status increased to 232 (99%. Updating the surveillance data required less than five minutes per notification.Referring to routine hospital admission data proved a useful and time-efficient surveillance strategy to increase the proportion of notifications with Aboriginal and Torres Strait Islander status. These data can then be used to better understand the current epidemiology of IPD. Aboriginal and Torres Strait Islander children aged 0–4 years have a two- to threefold higher rate of invasive pneumococcal disease than non-Aboriginal children, thus high levels of timely pneumococcal immunization coverage remain important for young Aboriginal and Torres Strait Islander children.

  5. Education, Power and Personal Biography: An Interview with Carlos Alberto Torres.

    Directory of Open Access Journals (Sweden)

    Armando Alcántara Santuario

    2000-05-01

    Full Text Available This conversation touches on crucial aspects of the work and research technique of one of Latin America’s most outstanding authors in the field of the political sociology of education. During this interview Professor Torres points out some of the principal themes of his research agenda, as well as what he considers Latin America’s most important contributions to the discussion of educational problems. Prof. Torres also gives a detailed account of the way he became interested in studying Paulo Freire’s work, which he has helped to disseminate in the USA. He explains the way in which he deals with theoretical and methodological problems in his research, as well as the advantages and disadvantages of being a Latin American studying international education while living and working in the United States. Finally, he makes some suggestions for those who are beginning their research on the educational problems of Mexico and Latin America.

  6. La Torre de Hanói y los Qn Grafos

    Directory of Open Access Journals (Sweden)

    Mª Milagros Latasa Asso

    2011-10-01

    Full Text Available La Torre de Hanói es uno de los hallazgos matemáticos más ingeniosos de la matemática recreativa. Gracias a una leyenda con tinte oriental hoy se conoce de modo universal. Se describen en este artículo las relaciones entre las soluciones del rompecabezas y los ciclos hamiltonianos en los grafos Qn.

  7. [The effects of TorR protein on initiation of DNA replication in Escherichia coli].

    Science.gov (United States)

    Yuan, Yao; Jiaxin, Qiao; Jing, Li; Hui, Li; Morigen, Morigen

    2015-03-01

    The two-component systems, which could sense and respond to environmental changes, widely exist in bacteria as a signal transduction pathway. The bacterial CckA/CtrA, ArcA/ArcB and PhoP/PhoQ two-component systems are associated with initiation of DNA replication and cell division, however, the effects of the TorS/TorR system on cell cycle and DNA replication remains unknown. The TorS/TorR system in Escherichia coli can sense changes in trimethylamine oxide (TMAO) concentration around the cells. However, it is unknown if it also affects initiation of DNA replication. We detected DNA replication patterns in ΔtorS and ΔtorR mutant strains by flow cytometry. We found that the average number of replication origins (oriCs) per cell and doubling time in ΔtorS mutants were the same while the average number of oriCs in ΔtorR mutants was increased compared with that in wild-type cells. These results indicated that absence of TorR led to an earlier initiation of DNA replication than that in wild-type cells. Strangely, neither overexpression of TorR nor co-expression of TorR and TorS could restore ΔtorR mutant phenotype to the wild type. However, overexpression of SufD in both wild type and ΔtorR mutants promoted initiation of DNA replication, while mutation of SufD delayed it in ΔtorR mutants. Thus, TorR may affect initiation of DNA replication indirectly through regulating gene expression of sufD.

  8. Inventarios de armamento y armaduras de una fortificación de La Alhambra: Torres Bermejas

    Directory of Open Access Journals (Sweden)

    Fernández-Rubio Hornillos, Gonzalo

    2014-12-01

    Full Text Available In the Alhambra archives and, to a lesser extent in the General Archive of the Madrid Palace, there is kept a very interesting documentation about the Torres Bermejas fortress in the Alhambra building. More than a dozen inventories allow us to know the arms and objets kept in Torres Bermejas since 1584. To find such a high number of inventories is something exceptional and they are made on the basis of a wide range of reasons: lawsuits, investitures of the new jailers or relationships of the goods existing in the Alhambra. We pretend to release those inventories and we will explain the combined circumstances concurred around them.En el Archivo de la Alhambra y, en menor medida, en el Archivo General del Palacio Real de Madrid, se conserva una documentación interesantísima sobre la fortaleza alhambreña de Torres Bermejas. Entre esta documentación se han localizado más de una docena de inventarios que nos permiten conocer las armas y objetos que hubo en Torres Bermejas. Estos inventarios abarcan un periodo que va desde 1584 hasta 1770. La localización de un número tan elevado de inventarios resulta algo excepcional y los mismos se elaboran por los más diversos motivos, como en tomas de posesión de nuevos alcaides o en las relaciones de bienes existentes en la Alhambra. Pretendemos dar a conocer estos inventarios y explicar diversas circunstancias que concurrieron en torno a esas armas, como robos, falta de pólvora, etc.

  9. Using Metasynthesis to Develop Sensitising Concepts to Understand Torres Strait Islander Migration

    Directory of Open Access Journals (Sweden)

    Vinnitta Patricia Mosby

    2013-03-01

    Full Text Available Emerging research indicates that more and more Indigenous peoples will be forced to migrate due to climate change. Current responses focus on mitigation and adaptation strategies. One such group, Torres Strait Islander people are already moving for other reasons and existing vulnerabilities compound levels of disadvantage when moving. It will be important to understand Torres Strait Islander people’s experiences of contemporary movements in order to inform policy development and facilitate the process of migration and resettlement as movement increases. A synthesis of existing studies would allow the development of sensitising concepts that could inform future research in the Torres Strait Islander context. This article presents a metasynthesis of six qualitative studies of the experiences of different Indigenous and minority groups at various stages of migration, displacement and resettlement. Articles were selected on contemporary movements (2001-2011 and importantly the inclusion of first person voice. Reciprocal translation was used to synthesise common themes and a core construct. The overarching construct that became apparent from the metasynthesis was ‘continuity of being’ through staying connected to self, family and culture. Three themes emerged: ‘freedom to be’, ‘staying close’ and ‘forming anchor’. These were enacted through people valuing their personal, social, religious and political freedom and recognising the importance of maintaining or forming strong social and family networks. When researching the experiences of Torres Strait Islanders it will be necessary to focus on motivations for moving, and understand the processes for staying connected to kin and homeland in order to achieve the desired outcomes of successful resettlement under conditions of uncertainty.

  10. Options for managing the sustainable use of green turtles: Perceptions of Hammond Islanders in Torres Strait

    Directory of Open Access Journals (Sweden)

    Grayson Jillian

    2010-01-01

    Full Text Available One of the largest populations of green turtles (Chelonia mydas in the world spends at least part of its life cycle in the remote Torres Strait between Australia and Papua New Guinea. This population is subjected to traditional harvests from geographically dispersed communities including along the northern and eastern coasts of Australia, Indonesia and south-western Pacific nations. In Torres Strait, green turtle hunting is classed as a traditional fishery and is guaranteed by Australian legislation (Native Title Act 1993 and the Torres Strait Treaty between Australia and Papua New Guinea that aims to protect the traditional lifestyle of the region′s indigenous peoples. To investigate the Islanders′ thoughts and aspirations regarding marine turtle management, we interviewed hunters and Islander Elders from the Hammond Island community in the Kaurareg nation of Kaiwalagal. Although not the Traditional Owners of the Kaiwalagal sea country in which they live and hunt, Hammond Islanders wish to be involved in the management of resources on which they depend, including marine turtles. They considered community-based processes to be important, especially the application of (1 cultural norms to the development of tools to achieve compliance and enforcement within the community, and (2 consensus-based decision-making amongst hunters and elders within the community, with regard to the use of more formal rules. However, the need for co-operation with other communities and stakeholders across scales was also recognised, particularly with regard to enforcement. Our results suggest that co-management is likely to be a more appropriate approach for managing green turtles in Torres Strait than either community-based management or government-driven management.

  11. Population biology of coral trout species in eastern Torres Strait: Implications for fishery management

    Science.gov (United States)

    Williams, Ashley J.; Currey, Leanne M.; Begg, Gavin A.; Murchie, Cameron D.; Ballagh, Aaron C.

    2008-09-01

    Coral trout ( Plectropomus spp.) are the main target species for commercial fishers in the eastern Torres Strait Reef Line Fishery (ETS RLF). The four species of coral trout known to occur in Torres Strait: Plectropomus leopardus, Plectropomus maculatus, Plectropomus areolatus and Plectropomus laevis are currently managed as a single species in Torres Strait, as there is no species-specific biological information available for the region which could be used to assess whether species differ in their response to fishing pressure. The aim of our study was to determine whether it is appropriate (biologically) to manage coral trout in the ETS RLF as a single species group or whether different management arrangements are required for some species. We used catch data and biological data from samples collected by commercial fishers to examine the distribution within Torres Strait and estimate a range of biological parameters for P. leopardus, P. maculatus and P. areolatus. Insufficient P. laevis samples were collected to reliably examine this species. Results indicated that the population biology, particularly the reproductive biology, of P. areolatus was substantially different to both P. leopardus and P. maculatus. Although it is difficult to predict the response to fishing, P. areolatus may be more vulnerable to fishing than P. leopardus and P. maculatus, due to the larger size at sex change observed for this species and the very low proportion of males protected by the current minimum size limit. Therefore, while the common management arrangements for P. leopardus and P. maculatus appear to be adequate for these species, separate management arrangements are needed for the sustainable harvest of P. areolatus populations in the ETS. Specifically, we recommend the introduction of a maximum size limit for P. areolatus, in addition to the current minimum size limit, which may allow a proportion of males some protection from fishing.

  12. UP-TORR: online tool for accurate and Up-to-Date annotation of RNAi Reagents.

    Science.gov (United States)

    Hu, Yanhui; Roesel, Charles; Flockhart, Ian; Perkins, Lizabeth; Perrimon, Norbert; Mohr, Stephanie E

    2013-09-01

    RNA interference (RNAi) is a widely adopted tool for loss-of-function studies but RNAi results only have biological relevance if the reagents are appropriately mapped to genes. Several groups have designed and generated RNAi reagent libraries for studies in cells or in vivo for Drosophila and other species. At first glance, matching RNAi reagents to genes appears to be a simple problem, as each reagent is typically designed to target a single gene. In practice, however, the reagent-gene relationship is complex. Although the sequences of oligonucleotides used to generate most types of RNAi reagents are static, the reference genome and gene annotations are regularly updated. Thus, at the time a researcher chooses an RNAi reagent or analyzes RNAi data, the most current interpretation of the RNAi reagent-gene relationship, as well as related information regarding specificity (e.g., predicted off-target effects), can be different from the original interpretation. Here, we describe a set of strategies and an accompanying online tool, UP-TORR (for Updated Targets of RNAi Reagents; www.flyrnai.org/up-torr), useful for accurate and up-to-date annotation of cell-based and in vivo RNAi reagents. Importantly, UP-TORR automatically synchronizes with gene annotations daily, retrieving the most current information available, and for Drosophila, also synchronizes with the major reagent collections. Thus, UP-TORR allows users to choose the most appropriate RNAi reagents at the onset of a study, as well as to perform the most appropriate analyses of results of RNAi-based studies.

  13. "Dancing with the stars" - astronomy and music in the Torres Strait

    CERN Document Server

    Hamacher, Duane W

    2016-01-01

    Song and dance are a traditional means of strengthening culture and passing knowledge to successive generations in the Torres Strait of northeastern Australia. Dances incorporate a range of apparatuses to enhance the performance, such as dance machines (Zamiyakal) and headdresses (Dhari). The dances, songs, headdresses and dance machines work together to transfer important knowledge about subsistence survival, social structure, and cultural continuity. This paper explores how celestial phenomena inspire and inform music and dance.

  14. An SK3 channel/nWASP/Abi-1 complex is involved in early neurogenesis.

    Directory of Open Access Journals (Sweden)

    Stefan Liebau

    Full Text Available BACKGROUND: The stabilization or regulated reorganization of the actin cytoskeleton is essential for cellular structure and function. Recently, we could show that the activation of the SK3-channel that represents the predominant SK-channel in neural stem cells, leads to a rapid local outgrowth of long filopodial processes. This observation indicates that the rearrangement of the actin based cytoskeleton via membrane bound SK3-channels might selectively be controlled in defined micro compartments of the cell. PRINCIPAL FINDINGS: We found two important proteins for cytoskeletal rearrangement, the Abelson interacting protein 1, Abi-1 and the neural Wiskott Aldrich Syndrome Protein, nWASP, to be in complex with SK3- channels in neural stem cells (NSCs. Moreover, this interaction is also found in spines and postsynaptic compartments of developing primary hippocampal neurons and regulates neurite outgrowth during early phases of differentiation. Overexpression of the proteins or pharmacological activation of SK3 channels induces obvious structural changes in NSCs and hippocampal neurons. In both neuronal cell systems SK3 channels and nWASP act synergistic by strongly inducing filopodial outgrowth while Abi-1 behaves antagonistic to its interaction partners. CONCLUSIONS: Our results give good evidence for a functional interplay of a trimeric complex that transforms incoming signals via SK3-channel activation into the local rearrangement of the cytoskeleton in early steps of neuronal differentiation involving nWASP and Abi-1 actin binding proteins.

  15. Monitorización de la Torre de Control del PAB con extensómetro de cable invar

    OpenAIRE

    2015-01-01

    La Torre de Control del Puerto de Barcelona está situada en el dique norte del llamado “Muelle de la Energía” en el puerto de Barcelona (España). Esta torre tiene diferentes tipos de antenas para el monitoreo de navegación y cuenta con una estación permanente GNSS en la azotea. La torre está construida en base a una estructura metálica y tiene 45 m de altura, está sometida a diversos movimientos como fluctuaciones periódicas por cambios de temperatura y viento e incluso a mo...

  16. A propósito de torres y de libros: la circularidad de las lecturas (de Borges a Rapunzel)

    OpenAIRE

    Eloy Martos

    2013-01-01

    La torre, la casa, el cuerpo, el mundo... son metáforas en suma del universo cuya correlación (en especial, cuerpo-casa-mundo) ha puesto de evidencia M. Eliade. El libro, los libros que están en la torre del texto de Borges son en realidad las historias que pueblan nuestra imaginación, de ahí esa vinculación simbólica, que percibimos en los textos citados de Borges, en el poema de A. Machado, en el cuento de Rapunzel o en las leyendas de torres encantadas, igual que en las actuales narrativas...

  17. Representation of Power of the Prince-Bishop Raimondo della Torre Patriarch of Aquileia (1273- 1299

    Directory of Open Access Journals (Sweden)

    Luca Demontis

    2013-12-01

    Full Text Available La rappresentazione del potere del principe-vescovo Raimondo della Torre, Patriarca di Aquileia (1273-1299 Raimondo della Torre, fratello del signore di Milano Napoleone della Torre, venne nominato patriarca di Aquileia nel 1273 da papa Gregorio X. Subito attuò un’attenta costruzione della propria immagine, organizzando il viaggio verso la sede patriarcale secondo una precisa simbologia biblica ispirata alle figure di Salomone e Davide; arrivò nei territori del patriarcato e assunse ufficialmente il potere con due cerimonie d’entrata nella capitale religiosa, Aquileia, e in quella politica, Cividale. Per rafforzare il suo potere e ottenere la collaborazione dei sudditi unì concrete azioni di buon governo a un’ampia comunicazione politica che comprendeva cerimonie d’investitura feudale rivolte a nobili, ministeriali e prelati del patriarcato, e ai principi confinanti; cerimonie di liberazione dei servi di masnada; una rinascita economica e architettonica delle città del patriarcato, depresse dalla potente feudalità friulana, con la costruzione di torri, palazzi, castelli, vie, piazze, fontane e la concessione di privilegi; la fondazione di una nuova città chiamata Milano di Raimondo; il ricorso all’iconografia del potere, alle monete, alle opere tessili, alla poesia e al teatro. La comunicazione politica gli permise di sviluppare un rapporto positivo con i suoi interlocutori raggiungendo i suoi obiettivi e creando una solida immagine di sé come principe-patriarca di Aquileia.

  18. Pharmacokinetic/Toxicity Properties of the New Anti-Staphylococcal Lead Compound SK-03-92

    Directory of Open Access Journals (Sweden)

    William R. Schwan

    2015-11-01

    Full Text Available Because of the potential of a new anti-staphylococcal lead compound SK-03-92 as a topical antibiotic, a patch, or an orally active drug, we sought to determine its safety profile and oral bioavailability. SK-03-92 had a high IC50 (125 μg/mL in vitro against several mammalian cell lines, and mice injected intraperiteonally at the highest dose did not exhibit gross toxicity (e.g., altered gait, ungroomed, significant weight loss. Single dose (100 μg/g pharmacokinetic (PK analysis with formulated SK-03-92 showed that peak plasma concentration (1.64 μg/mL was achieved at 20–30 min. Oral relative bioavailability was 8%, and the drug half-life was 20–30 min, demonstrating that SK-03-92 is likely not a candidate for oral delivery. Five-day and two-week PK analyses demonstrated that SK-03-92 plasma levels were low. Multi-dose analysis showed no gross adverse effects to the mice and a SK-03-92 peak plasma concentration of 2.12 μg/mL with the presence of significant concentrations of breakdown products 15 min after dosing. SK-03-92 appeared to be very safe based on tissue culture and mouse gross toxicity determinations, but the peak plasma concentration suggests that a pro-drug of SK-03-92 or preparation of analogs of SK-03-92 with greater bioavailability and longer half-lives are warranted.

  19. Pharmacokinetics and tissue distribution of a novel PDE5 inhibitor,SK-3530, in rats

    Institute of Scientific and Technical Information of China (English)

    Hye-hyun YOO; Nam-sun KIM; Guang-jin Im; Dong-hyun KIM

    2007-01-01

    Aim: To investigate the pharmacokinetic profile and tissue distribution of a novel phosphodiesterase type 5 inhibitor, 5-ethyl-2-{5-[4-(2-hydroxy-ethyl)-piperazine-1-sulfonyl]-2-propoxy-phenyl }-7-propyl-3,5-dihydro-pyrrolo(3,2-d)pyrimidin-4-one (SK-3530), in rats after administration of the 14C-labeled compound. Methods:The pharmacokinetic parameters of SK-3530 were measured based on the total radioactivity and parent SK-3530 concentration in rat plasma after intravenous and oral administration. The tissue distribution of total radioactivity after a single oral administration of [14C]SK-3530 at a dose of 40 mg/kg was assayed. The plasma protein binding rates of SK-3530 were assessed by in vitro and ex vivo assay. Results: The total radioactivity profiles showed linear pharmacokinetics.The maximum plasma concentration and area under the curve of the parent SK3530 were 10%-20% compared to those of the total radioactivity. After the oral admin-istration of [14C]SK-3530, the radioactivity was widely distributed in all tissues,and the tissue/plasma ratio of the radioactivity 1 h after administration was calcu-lated as 0.5-2.6 with the exception of excretory organs. A relatively high penetra-tion was shown in the adrenal glands, liver, and lung. In vitro and ex vivo plasma protein binding assay by ultrafiltration showed a considerably high binding rate of more than 97%. Conclusion: SK-3530 was relatively well absorbed in the gas-trointestinal tract and showed linear pharmacokinetics over the investigated dose range. SK-3530 had low oral bioavailability due to a high, first-pass metabolism.

  20. File list: InP.Brs.10.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Brs.10.AllAg.SK-BR-3 hg19 Input control Breast SK-BR-3 SRX750675,SRX155756,SRX4...69109 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Brs.10.AllAg.SK-BR-3.bed ...

  1. File list: InP.Brs.20.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Brs.20.AllAg.SK-BR-3 hg19 Input control Breast SK-BR-3 SRX750675,SRX155756,SRX4...69109 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Brs.20.AllAg.SK-BR-3.bed ...

  2. File list: InP.Brs.05.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  3. File list: InP.Brs.50.AllAg.SK-BR-3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Brs.50.AllAg.SK-BR-3 hg19 Input control Breast SK-BR-3 SRX750675,SRX469109,SRX1...55756 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Brs.50.AllAg.SK-BR-3.bed ...

  4. Therapeutic doses of SkQ1 do not induce cytochromes P450 in rat liver.

    Science.gov (United States)

    Myasoedova, K N; Silachev, D N

    2014-10-01

    The effect of SkQ1 (a mitochondria-targeted antioxidant) on the level of cytochromes P450 in rat liver was studied. It was found that administration of therapeutic dose of SkQ1 with drinking water for 5 days (250 nmol/kg of body weight per day) did not alter the level of cytochromes P450. Under the same conditions, the standard dose of phenobarbital used for the induction of cytochromes P450 caused the 2.7-fold increase in the content of these cytochromes. We conclude that therapeutic doses of SkQ1 do not induce cytochromes P450 in rats.

  5. D Model of the Torres Syncline in the PARANÁ-ETENDEKA Province, South Atlantic Margin

    Science.gov (United States)

    Jerram, D. A.; Waichel, B. L.; Lima, E. F. D.; Rossetti, L. D. M. M.; Bueno, G. V.; Viana, A. R.; Barreto, C. J.

    2015-12-01

    The Paraná-Etendeka Volcanic Province (PEVP) is Early Cretaceous in age and precedes the fragmentation of the south Gondwana. These volcanic rocks cover an area in excess of 1,200,000 km2 and can reach a maximum thickness of 1,700 m. The PEVP is composed mainly of tholeiitic basalts and subordinately by andesites and rhyolites/quartz-latites. Recently new models considering physical characteristics of the lava flow stratigraphy on both sides, Africa and South America, have been built providing a new vision of the volcanic stratigraphy and flow morphologies inside the province. This work presents a 3D model of the volcanic sequence at theTorres Syncline (Brazil). The 3D model was build using the PETREL E&P Software Platform. Geological sections based on field data and Log data from Paraná Basin (Gama-ray and soniclogs) were used to build the model. The Torres Syncline is a tectonic structure located in southern Brazil with the main orientation NW-SE. In Torres Syncline the basal portion is characterized by pahoehoe lava flows in a compound braided architecture, followed by rubbly lavas in a tabular classic architecture, and in the upper portions silicic units interbedded with basaltic lavas. The central portion of the volcanic pile is characterized by thick tabular lavas with rubbly tops (25-50 m thick). These flows have an internal structure divided in four parts: a smooth vesicular base, aphanitic massive cores with irregular joints, upper vesicular portion and a rubbly top. This unit is thicker along of the Torres Syncline and represents the main phase of the volcanism. Silicic units occur in the upper parts of the PEVP stratigraphy and include lava domes interbedded with rubbly flows and thick tabular flows. The Torres Syncline and Huab constituted one Basin features single active structure in the Early Cretaceous and during the main rifting phase. The onset of the volcanism was characterized by low effusion rate eruptions over the paleoerg, forming pahoehoe

  6. Expression of postsynaptic Ca2+-activated K+ (SK) channels at C-bouton synapses in mammalian lumbar α-motoneurons

    Science.gov (United States)

    Deardorff, Adam S; Romer, Shannon H; Deng, Zhihui; Bullinger, Katie L; Nardelli, Paul; Cope, Timothy C; Fyffe, Robert E W

    2013-01-01

    Small-conductance calcium-activated potassium (SK) channels mediate medium after-hyperpolarization (AHP) conductances in neurons throughout the central nervous system. However, the expression profile and subcellular localization of different SK channel isoforms in lumbar spinal α-motoneurons (α-MNs) is unknown. Using immunohistochemical labelling of rat, mouse and cat spinal cord, we reveal a differential and overlapping expression of SK2 and SK3 isoforms across specific types of α-MNs. In rodents, SK2 is expressed in all α-MNs, whereas SK3 is expressed preferentially in small-diameter α-MNs; in cats, SK3 is expressed in all α-MNs. Function-specific expression of SK3 was explored using post hoc immunostaining of electrophysiologically characterized rat α-MNs in vivo. These studies revealed strong relationships between SK3 expression and medium AHP properties. Motoneurons with SK3-immunoreactivity exhibit significantly longer AHP half-decay times (24.67 vs. 11.02 ms) and greater AHP amplitudes (3.27 vs. 1.56 mV) than MNs lacking SK3-immunoreactivity. We conclude that the differential expression of SK isoforms in rat and mouse spinal cord may contribute to the range of medium AHP durations across specific MN functional types and may be a molecular factor distinguishing between slow- and fast-type α-MNs in rodents. Furthermore, our results show that SK2- and SK3-immunoreactivity is enriched in distinct postsynaptic domains that contain Kv2.1 channel clusters associated with cholinergic C-boutons on the soma and proximal dendrites of α-MNs. We suggest that this remarkably specific subcellular membrane localization of SK channels is likely to represent the basis for a cholinergic mechanism for effective regulation of channel function and cell excitability. PMID:23129791

  7. Un lienzo de la Inmaculada de Matías de Torres en la Colegiata de Talavera de la Reina

    Directory of Open Access Journals (Sweden)

    Nicolau Castro, Juan

    2014-03-01

    Full Text Available The unpublished painting of the Immaculate Conception in the Collegiate Church of Talavera de la Reina can be attributed to the 17th century artist Matías de Torres.Un lienzo de la Inmaculada en la Colegiata de Talavera de la Reina, hasta ahora inédito, puede ser atribuido al pintor del siglo XVII Matías de Torres.

  8. Influence of source batch Sk dispersion on dosimetry for prostate cancer treatment with permanent implants

    DEFF Research Database (Denmark)

    Nunez-Cumplido, Eduardo; Perez-Calatayud, J; Casares-Magaz, O;

    2015-01-01

    PURPOSE: In clinical practice, specific air kerma strength (SK) value is used in treatment planning system (TPS) permanent brachytherapy implant calculations with (125)I and (103)Pd sources; in fact, commercial TPS provide only one SK input value for all implanted sources and the certified shipment...... is to examine the impact of SK dispersion on typical implant parameters that are used to evaluate the dose volume histogram (DVH) for both planning target volume (PTV) and organs at risk (OARs). METHODS: The authors have developed a new algorithm to compute dose distributions with different SK values for each...... source. Three different prostate volumes (20, 30, and 40 cm(3)) were considered and two typical commercial sources of different radionuclides were used. Using a conventional TPS, clinically accepted calculations were made for (125)I sources; for the palladium, typical implants were simulated. To assess...

  9. Control of sexuality by the sk1-encoded UDP-glycosyltransferase of maize.

    Science.gov (United States)

    Hayward, Andrew P; Moreno, Maria A; Howard, Thomas P; Hague, Joel; Nelson, Kimberly; Heffelfinger, Christopher; Romero, Sandra; Kausch, Albert P; Glauser, Gaétan; Acosta, Ivan F; Mottinger, John P; Dellaporta, Stephen L

    2016-10-01

    Sex determination in maize involves the production of staminate and pistillate florets from an initially bisexual floral meristem. Pistil elimination in staminate florets requires jasmonic acid signaling, and functional pistils are protected by the action of the silkless 1 (sk1) gene. The sk1 gene was identified and found to encode a previously uncharacterized family 1 uridine diphosphate glycosyltransferase that localized to the plant peroxisomes. Constitutive expression of an sk1 transgene protected all pistils in the plant, causing complete feminization, a gain-of-function phenotype that operates by blocking the accumulation of jasmonates. The segregation of an sk1 transgene was used to effectively control the production of pistillate and staminate inflorescences in maize plants.

  10. Characterization of CD4 and CD8 T cell responses in MuSK myasthenia gravis.

    Science.gov (United States)

    Yi, J S; Guidon, A; Sparks, S; Osborne, R; Juel, V C; Massey, J M; Sanders, D B; Weinhold, K J; Guptill, J T

    2014-08-01

    Muscle specific tyrosine kinase myasthenia gravis (MuSK MG) is a form of autoimmune MG that predominantly affects women and has unique clinical features, including prominent bulbar weakness, muscle atrophy, and excellent response to therapeutic plasma exchange. Patients with MuSK MG have predominantly IgG4 autoantibodies directed against MuSK on the postsynaptic muscle membrane. Lymphocyte functionality has not been reported in this condition. The goal of this study was to characterize T cell responses in patients with MuSK MG. Intracellular production of IFN-gamma, TNF-alpha, IL-2, IL-17, and IL-21 by CD4+ and CD8+ T cells was measured by polychromatic flow cytometry in peripheral blood samples from 11 Musk MG patients and 10 healthy controls. Only one MuSK MG patient was not receiving immunosuppressive therapy. Regulatory T cells (Treg) were also included in our analysis to determine if changes in T cell function were due to altered Treg frequencies. CD8+ T cells from MuSK MG patients had higher frequencies of polyfunctional responses than controls, and CD4+ T cells had higher IL-2, TNF-alpha, and IL-17. MuSK MG patients had a higher percentage of CD4+ T cells producing combinations of IFN-gamma/IL-2/TNF-gamma, TNF-alpha/IL-2, and IFN-gamma/TNF-alpha. Interestingly, Treg numbers and CD39 expression were not different from control values. MuSK MG patients had increased frequencies of Th1 and Th17 cytokines and were primed for polyfunctional proinflammatory responses that cannot be explained by a defect in CD39 expression or Treg number.

  11. Efficacy and Safety of Intacs SK in Moderate to Severe Keratoconus

    OpenAIRE

    El-Moatassem Kotb, Ahmed M.; Mohamed Hantera

    2013-01-01

    Purpose: To examine the safety and efficacy of Intacs SK for moderate to severe keratoconus (KC) using femtosecond technology. Materials and Methods: This prospective, non-comparative study included 37 contact lens intolerant keratoconic eyes (stage II-III) of 24 patients who underwent femtosecond-assisted Intacs SK implantation. Inclusion criteria were mean K readings 400 μm at the incision site, mesopic pupil

  12. The Analgesic Effect of the Mitochondria-Targeted Antioxidant SkQ1 in Pancreatic Inflammation

    Directory of Open Access Journals (Sweden)

    Maximilian Weniger

    2016-01-01

    Full Text Available Background. Chronic pancreatitis is one of the main risk factors for pancreatic cancer. In acute and chronic pancreatitis, oxidative stress is thought to play a key role. In this respect, the recently described mitochondria-targeted antioxidant SkQ1 effectively scavenges reactive oxygen species at nanomolar concentrations. Therefore, we aimed to characterize the influence of SkQ1 on tissue injury and pain in acute and chronic pancreatitis. Methods. Both acute and chronic pancreatitis were induced in C57BL/6 mice by intraperitoneal cerulein injections and treatment with SkQ1 was carried out by peroral applications. Hyperalgesia was assessed by behavioral observation and measurement of abdominal mechanical sensitivity. Blood serum and pancreatic tissue were harvested for analysis of lipase and histology. Results. SkQ1 did not influence pain, serological, or histological parameters of tissue injury in acute pancreatitis. In chronic pancreatitis, a highly significant reduction of pain-related behavior (p<0.0001 was evident, but histological grading revealed increased tissue injury in SkQ1-treated animals (p=0.03. Conclusion. After SkQ1 treatment, tissue injury is not ameliorated in acute pancreatitis and increased in chronic pancreatitis. However, we show an analgesic effect in chronic pancreatitis. Further studies will need to elucidate the risks and benefits of mitochondria-targeted antioxidants as an analgesic.

  13. Sustaining sleep spindles through enhanced SK2 channel activity consolidates sleep and elevates arousal threshold

    Science.gov (United States)

    Wimmer, Ralf D.; Astori, Simone; Bond, Chris T.; Rovó, Zita; Chatton, Jean-Yves; Adelman, John P.; Franken, Paul; Lüthi, Anita

    2013-01-01

    Sleep spindles are synchronized 11–15 Hz electroencephalographic (EEG) oscillations predominant during non-rapid-eye-movement sleep (NREMS). Rhythmic bursting in the reticular thalamic nucleus (nRt), arising from interplay between Cav3.3-type Ca2+ channels and Ca2+-dependent small-conductance-type 2 (SK2) K+ channels, underlies spindle generation. Correlative evidence indicates that spindles contribute to memory consolidation and protection against environmental noise in human NREMS. Here, we describe a molecular mechanism through which spindle power is selectively extended and we probed the actions of intensified spindling in the naturally sleeping mouse. Using electrophysiological recordings in acute brain slices from SK2 channel-over-expressing (SK2-OE) mice, we found that nRt bursting was potentiated and thalamic circuit oscillations were prolonged. Moreover, nRt cells showed greater resilience to transit from burst to tonic discharge in response to gradual depolarization, mimicking transitions out of NREMS. Compared to wild-type littermates, chronic EEG recordings of SK2-OE mice contained less fragmented NREMS, while the NREMS EEG power spectrum was conserved. Furthermore, EEG spindle activity was prolonged at NREMS exit. Finally, when exposed to white noise, SK2-OE mice needed stronger stimuli to arouse. Increased nRt bursting thus strengthens spindles and improves sleep quality through mechanisms independent of EEG slow-waves (< 4 Hz), suggesting SK2 signaling as a new potential therapeutic target for sleep disorders and for neuropsychiatric diseases accompanied by weakened sleep spindles. PMID:23035101

  14. Sustaining sleep spindles through enhanced SK2-channel activity consolidates sleep and elevates arousal threshold.

    Science.gov (United States)

    Wimmer, Ralf D; Astori, Simone; Bond, Chris T; Rovó, Zita; Chatton, Jean-Yves; Adelman, John P; Franken, Paul; Lüthi, Anita

    2012-10-03

    Sleep spindles are synchronized 11-15 Hz electroencephalographic (EEG) oscillations predominant during nonrapid-eye-movement sleep (NREMS). Rhythmic bursting in the reticular thalamic nucleus (nRt), arising from interplay between Ca(v)3.3-type Ca(2+) channels and Ca(2+)-dependent small-conductance-type 2 (SK2) K(+) channels, underlies spindle generation. Correlative evidence indicates that spindles contribute to memory consolidation and protection against environmental noise in human NREMS. Here, we describe a molecular mechanism through which spindle power is selectively extended and we probed the actions of intensified spindling in the naturally sleeping mouse. Using electrophysiological recordings in acute brain slices from SK2 channel-overexpressing (SK2-OE) mice, we found that nRt bursting was potentiated and thalamic circuit oscillations were prolonged. Moreover, nRt cells showed greater resilience to transit from burst to tonic discharge in response to gradual depolarization, mimicking transitions out of NREMS. Compared with wild-type littermates, chronic EEG recordings of SK2-OE mice contained less fragmented NREMS, while the NREMS EEG power spectrum was conserved. Furthermore, EEG spindle activity was prolonged at NREMS exit. Finally, when exposed to white noise, SK2-OE mice needed stronger stimuli to arouse. Increased nRt bursting thus strengthens spindles and improves sleep quality through mechanisms independent of EEG slow waves (<4 Hz), suggesting SK2 signaling as a new potential therapeutic target for sleep disorders and for neuropsychiatric diseases accompanied by weakened sleep spindles.

  15. Mitochondria-Targeted Antioxidant SkQ1 Improves Dermal Wound Healing in Genetically Diabetic Mice.

    Science.gov (United States)

    Demyanenko, Ilya A; Zakharova, Vlada V; Ilyinskaya, Olga P; Vasilieva, Tamara V; Fedorov, Artem V; Manskikh, Vasily N; Zinovkin, Roman A; Pletjushkina, Olga Yu; Chernyak, Boris V; Skulachev, Vladimir P; Popova, Ekaterina N

    2017-01-01

    Oxidative stress is widely recognized as an important factor in the delayed wound healing in diabetes. However, the role of mitochondrial reactive oxygen species in this process is unknown. It was assumed that mitochondrial reactive oxygen species are involved in many wound-healing processes in both diabetic humans and animals. We have applied the mitochondria-targeted antioxidant 10-(6'-plastoquinonyl)decyltriphenylphosphonium (SkQ1) to explore the role of mitochondrial reactive oxygen species in the wound healing of genetically diabetic mice. Healing of full-thickness excisional dermal wounds in diabetic C57BL/KsJ-db(-)/db(-) mice was significantly enhanced after long-term (12 weeks) administration of SkQ1. SkQ1 accelerated wound closure and stimulated epithelization, granulation tissue formation, and vascularization. On the 7th day after wounding, SkQ1 treatment increased the number of α-smooth muscle actin-positive cells (myofibroblasts), reduced the number of neutrophils, and increased macrophage infiltration. SkQ1 lowered lipid peroxidation level but did not change the level of the circulatory IL-6 and TNF. SkQ1 pretreatment also stimulated cell migration in a scratch-wound assay in vitro under hyperglycemic condition. Thus, a mitochondria-targeted antioxidant normalized both inflammatory and regenerative phases of wound healing in diabetic mice. Our results pointed to nearly all the major steps of wound healing as the target of excessive mitochondrial reactive oxygen species production in type II diabetes.

  16. The Jurassic of Denmark and Greenland: The Jurassic of Skåne, southern Sweden

    Directory of Open Access Journals (Sweden)

    Sivhed, Ulf

    2003-10-01

    Full Text Available In Sweden, Jurassic strata are restricted to Skåne and adjacent offshore areas. Jurassic sedimentary rocks predominantly comprise sandy to muddy siliciclastics, with subordinate coal beds andfew carbonate-rich beds. During Mesozoic times, block-faulting took place in the Sorgenfrei–Tornquist Zone, a tectonic zone which transects Skåne in a NW–SE direction. The Jurassic depositionalenvironments in Skåne were thus strongly influenced by uplift and downfaulting, and to some extent by volcanism. Consequently, the sedimentary record reveals evidence of numerous transgressions, regressions and breaks in sedimentation. Relative sea-level changes played a significant role in controlling the facies distribution, as deposition mainly took place in coastal plain to shallow shelf environments.The alluvial deposits in Skåne include floodplain palaeosols, autochthonous coals, overbank sandstones, and stream channel pebbly sandstones. Restricted marine strata comprise intertidalheteroliths with mixed freshwater and marine trace fossil assemblages, and intertidal delta distributary channel sandstones. Shallow marine sediments encompass subtidal and shoreface sandstoneswith herringbone structures, and bioturbated mudstones with tempestite sandstones. Offshore deposits typically comprise extensively bioturbated muddy sandstones.Floral remains, palaeopedology, clay mineralogy and arenite maturity indicate a warm and humid climate in Skåne throughout the Jurassic, possibly with slightly increasing aridity towards the end of the period. Most Jurassic strata in Skåne have been subjected to mild burial diagenesis, and the petroleum generative window has rarely been reached.

  17. UBE3A Regulates Synaptic Plasticity and Learning and Memory by Controlling SK2 Channel Endocytosis

    Directory of Open Access Journals (Sweden)

    Jiandong Sun

    2015-07-01

    Full Text Available Gated solely by activity-induced changes in intracellular calcium, small-conductance potassium channels (SKs are critical for a variety of functions in the CNS, from learning and memory to rhythmic activity and sleep. While there is a wealth of information on SK2 gating, kinetics, and Ca2+ sensitivity, little is known regarding the regulation of SK2 subcellular localization. We report here that synaptic SK2 levels are regulated by the E3 ubiquitin ligase UBE3A, whose deficiency results in Angelman syndrome and overexpression in increased risk of autistic spectrum disorder. UBE3A directly ubiquitinates SK2 in the C-terminal domain, which facilitates endocytosis. In UBE3A-deficient mice, increased postsynaptic SK2 levels result in decreased NMDA receptor activation, thereby impairing hippocampal long-term synaptic plasticity. Impairments in both synaptic plasticity and fear conditioning memory in UBE3A-deficient mice are significantly ameliorated by blocking SK2. These results elucidate a mechanism by which UBE3A directly influences cognitive function.

  18. El marco conceptual relacionado con la calidad: una torre de Babel

    Directory of Open Access Journals (Sweden)

    Miryam Escobar Valencia

    2013-12-01

    Full Text Available Según el Génesis de la Biblia (s.f., ed. 2009, la Torre de Babel fue construida por la humanidad para alcanzar el cielo, por lo que Yahvé confundió la lengua de los hombres. Este acontecimiento les llevó a dejar la torre inacabada y a que se marcharan en todas direcciones*; al parecer, esta es una realidad cuando se habla de calidad. En este texto se exponen las definiciones sobre calidad y otros conceptos relacionados, elaborados por autores emblemáticos del tema, incluyendo el impacto que esta noción tiene en el montaje de sistemas de gestión y formalización a través de procesos de certificación. En primera instancia, se aborda el concepto de calidad, incluyendo elementos históricos claves para su comprensión como la calidad en Japón, la calidad total, la calidad y la administración y los antecedentes del sistema de gestión. Luego se realiza una presentación de argumentos, base de la reflexión, que aportan a la necesidad imperiosa de la comprensión de la calidad, como asunto clave debido a su abordaje por presión o por voluntad, hacia un camino de certificación organizacional. Esto con el fin de abogar por la creación de un marco -campo- conceptual fortalecido que hoy se ha convertido en un paradigma, eliminando así la Torre de Babel que se pretende exponer

  19. Delivery of eye and vision services in Aboriginal and Torres Strait Islander primary health care centres

    Directory of Open Access Journals (Sweden)

    Anthea M Burnett

    2016-12-01

    Full Text Available Background: Routine eye and vision assessments are vital for the detection and subsequent management of vision loss, which is particularly important for Aboriginal and Torres Strait Islander people, who face higher rates of vision loss than other Australians. In order to guide improvements, this paper will describe patterns, variations and gaps in these eye and vision assessments for Aboriginal and Torres Strait Islander people. Methods: Clinical audits from 124 primary health care centres (sample size 15,175 from five Australian States and Territories were conducted during 2005-2012. Main outcome measure was adherence to current guidelines for delivery of eye and vision assessments to adults with diabetes, those without a diagnosed major chronic disease and children attending primary health care centres. Results: Overall delivery of recommended eye and vision assessments varied widely between health centres. Of the adults with diabetes, 45% had a visual acuity assessment recorded within the previous 12 months (health centre range 0-88%, and 33% had a retinal examination recorded (health centre range 0-73%. Of the adults with no diagnosed major chronic disease, 31% had a visual acuity assessment recorded within the previous two years (health centre range 0-30%, and 13% had received an examination for trichiasis (health centre range 0-40%. In children, 49% had a record of a vision assessment (health centre range 0-97%, and 25% had a record of an examination for trachoma within the previous 12 months (health centre range 0-63%. Conclusions: There was considerable range, and variation in the recorded delivery of scheduled eye and vision assessments across health centres. Sharing the successful strategies of the better-performing health centres to support focused improvements in key areas of need may increase overall rates of eye examinations – important for the timely detection, referral and treatment of eye conditions affecting Aboriginal and

  20. Torre de control. aeropuerto de Fort Worth - Dallas - Texas – (EE. UU.

    Directory of Open Access Journals (Sweden)

    Becket, Welton

    1975-11-01

    Full Text Available The control tower consists of a low building and the actual tower. The 2,400 m2 surface space of the concrete base building is occupied by administrative offices, training facilities, shops and reception area. The structural system of the 60 m high tower is made up of 4 service cores, completed by a cabin and a control equipment level. The service cores are hollow modular units, equally of concrete, which house the elevator, stairs, power and communication cables. The cabin that crowns the tower is an 11-sided polygon. Immediately below it is the equipment level, formed by four quadrants the configuration of which guarantees 360 degree visibility. The control tower is the focal point and nerve centre of the activities at the airport.Está formada por un cuerpo bajo y la torre propiamente dicha. La base, realizada de hormigón, distribuye, en sus 2.400 m2 de superficie: oficinas administrativas, locales de enseñanza, tiendas y una zona de recepción. La torre, de 60 m de altura, se diseñó como un sistema estructural constituido por cuatro núcleos de servicio, rematada por una cabina y una planta para los equipos de control. Los núcleos son unidades modulares huecas, también de hormigón, que albergan en su interior un ascensor, las escaleras, las instalaciones de electricidad y comunicaciones. La cabina que corona el edificio es un polígono regular de once lados. Inmediatamente debajo de ella se encuentra el nivel de equipos, formado por cuatro cuadrantes cuya configuración asegura una visibilidad de 360°. La torre es el punto focal y centro neurálgico de todas las actividades del aeropuerto.

  1. La imagen literaria del artista de vanguardia en el siglo XX : Jusep Torres Campalans

    OpenAIRE

    Fernández Martínez, Dolores

    2011-01-01

    Como resultado de una investigación previa en la memoria de licenciatura (la imagen del artista en la cultura de masas), esta tesis viene a demostrar la utilidad de las novelas sobre artistas como fuente para el historiador del arte. Jusep Torres Campalans no solamente nos da una visión del círculo de Picasso antes de 1914, refleja las fobias y las filias de Max Aub y su generación al tiempo que supone una critica de la historia, la biografía y las monografías de arte.

  2. Un homenaje fallido a Carroll: La torre sin fin, de Silvina Ocampo

    Directory of Open Access Journals (Sweden)

    Natalia Biancotto

    2015-12-01

    Full Text Available The children’s novel La torre sin fin, by Silvina Ocampo, first published in Madrid in 1986 (Alfaguara and then published posthumously in 2007 in Argentina (Sudamericana, is one of the stories of the author in which is more obvious than in any other the rewriting of the Carrollian topics and problems. This paper proposes to describe the way in which this short novel of Ocampo, which presents an explicit homage to Carroll’s Alices, constitutes a simplified and schematic version of Carrollian nonsense that turns to be a failed tribute.

  3. Reticulated acanthoma with sebaceous differentiation: another sebaceous neoplasm associated with Muir-Torre syndrome?

    Science.gov (United States)

    Shon, Wonwoo; Wolz, Michael M; Newman, Catherine C; Bridges, Alina G

    2014-11-01

    Reticulated acanthoma with sebaceous differentiation (RASD) represents a rare benign cutaneous epithelial neoplasm with sebaceous differentiation. There has been much speculation about the relationship between RASD and Muir-Torre syndrome (MTS). We report a 53 year-old man who presented with RASD in addition to a prior history of sebaceous adenomas. Immunohistochemically, the tumour cells in the RASD and sebaceous adenomas showed a significantly reduced MSH6 protein expression, whereas there was no loss of MLH1, MSH2 and PMS2. This benign neoplasm, which can be mistaken for various other cutaneous lesions with sebaceous differentiation, deserves wider recognition for its possible association with MTS.

  4. Reticulated acanthoma with sebaceous differentiation. Lack of association with Muir-Torre syndrome.

    Science.gov (United States)

    Haake, Dana L; Minni, John P; Nowak, Michael; Abenoza, Pascual; Nousari, Carlos H

    2009-06-01

    We hereby report a case of a reticulated acanthoma with sebaceous differentiation (RASD), a rare and often mislabeled benign lesion that is characterized by epidermal acanthosis and clusters of sebocytes in a reticulated seborrheic keratosis-like pattern. The presence of multiple sebaceous tumors, most notably cystic sebaceous adenomas and keratoacanthomas, has been associated with Muir-Torre syndrome (MTS). Although very rare, cases of RASD have been reported with MTS, which potentially offers profound clinical significance to this neoplasm. This case further supports the lack of association of MTS with RASD.

  5. Torre Llagostera : sistema constructivo, Huerto de las Bolas, Jarden del Modernismo

    OpenAIRE

    Vera Fornet, Miriam

    2012-01-01

    El objetivo principal del presente P.F.C. es la de profundizar en el estudio de una de las villas más imporatantes del Campo de Cartagena, pues a día de hoy todavía se mantiene el diseño que realizó el arquitecto Víctor Beltrí en gran parte de la villa, además de los elementos que la complementan como son las acequias, los bancos y el estanque. Este trabajo sirve para conocer los sistemas constructivos de la principal edificación de la finca, la denominada -Torre Llagostera-, con el fin de co...

  6. Seroprevalencia de la Brucelosis en Pequeños Rumiantes. Municipio Torres. Año 2008 (Seroprevalence of Brucellosis in Small Ruminants. Municipality Torres. Year 2008

    Directory of Open Access Journals (Sweden)

    Javitt J., Milva

    2009-08-01

    Full Text Available ResumenLa finalidad del presente estudio epidemiológico fue estimar laprevalencia de Brucelosis en pequeños rumiantes criados bajo unsistema extensivo, en las parroquias del Municipio Torres. EstadoLara durante el año 2008. Se trabajó con 7628 muestras en total, las6.846 muestras caprinas y las 782 muestras ovinas que fueronremitidas por los médicos veterinarios de ejercicio libre al Laboratorio Regional de Diagnóstico Zoosanitario del Servicio Autónomo de Sanidad Agropecuaria (SASA Lara ubicado en Carora, durante el año 2008, sin ninguna historia de vacunación. Todas las muestras fueron examinadas para la detección de anticuerpos a Brucella usando un antígeno de B. abortus cepa 1119-3, en pruebas de aglutinación (placa, tubo, 2-Mercaptoetanol. Del total de muestras procesadas resultaron con títulos positivos 182 caprinos (2,69% y 17 ovinos (2,17%; y 796 caprinos (11,63% y 84 ovinos (10,74%presentaron reacciones dudosas; dando una prevalencia en estegrupo de animales, de 0,027 para caprinos y de 0,022 para ovinos.SummaryThe aim of this epidemiological study was to estimate the prevalenceof brucellosis in small ruminants reared under an extensive system inthe parishes of the municipality of Torres, Lara State, during 2008. In total, 7628 sera samples were analyzed, 6846 and 782 samples fromgoats and sheep, respectively were taken for free exerciseveterinarians and transported to Animal Health Diagnostic Laboratory at Regional Autonomous Agricultural Health Service (SASA, Laralocated in Carora, during 2008. The sampled animals have no historyof vaccination. All samples were examined to detect Brucellaantibodies using an antigen of B. abortus strain 1119-3, inagglutination tests (plate, tube, 2 - mercaptoethanol. Out of 6846processed goat samples, 182 showed positive titles (2.69% and796goats (11.63% were suspected. Out of 782, 17 sera sheep shownpositive titles (2.17% and 84 sheep (10.74% had doubtfulreactions. The prevalence obtained

  7. Network excitability in a model of chronic temporal lobe epilepsy critically depends on SK channel-mediated AHP currents.

    Science.gov (United States)

    Schulz, Robert; Kirschstein, Timo; Brehme, Hannes; Porath, Katrin; Mikkat, Ulrike; Köhling, Rüdiger

    2012-01-01

    Hippocampal CA1 pyramidal neurons generate an after-hyperpolarization (AHP) whose medium component is thought to be generated by small-conductance Ca(2+)-activated K(+) channels (SK channels). Neuronal excitability is increased in epilepsy, and the AHP in turn is fundamentally involved in regulation of cellular excitability. We therefore investigated the involvement of the SK channel-mediated AHP in controlling cell and network excitability in the pilocarpine model epilepsy. Both acutely isolated CA1 pyramidal cells and isolated hippocampal slices were investigated in terms of the impact of SK channel-mediated AHP on hyperexcitability. Our findings show that pilocarpine-treated chronically epileptic rats exhibit significantly reduced SK channel-mediated hyperpolarizing outward current which was accompanied by a significant decrease in the somatic AHP. Paradoxically, inhibiting SK channels strongly exacerbated 0-Mg(2+)-induced epileptiform activity in slices from pilocarpine-treated animals, while having a significantly smaller effect in control tissue. This suggests that in chronically epileptic tissue, network excitability very critically depends on the remaining SK-channel mediated AHP. Additional real-time RT-PCR and semiquantitative Western blot experiments revealed that both the SK2 channel transcript and protein were significantly downregulated in the epileptic CA1 region. We conclude that SK2 channels are down-regulated in chronic epilepsy underlying the impaired SK channel function in CA1 pyramidal cells, and a further reduction of the remaining critical mass of SK channels results in an acute network decompensation.

  8. Cómo se construye una torre mudéjar. La torre de la iglesia de San Pedro el Viejo de Madrid

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    Caballero Zoreda, Luis

    2004-12-01

    Full Text Available The Mudejar tower of San Pedro el Viejo de Madrid offers, thanks to various modern-era and contemporary interventions, the possibility of analyzing its unknown construction process and construction tecniques combining brick and limestone stonework. A wall of brick and mud was used as the formwork of limestone walls and then used as a base for the first sections and staircase. The wood and brick walls on view were also used as formwork.La torre mudéjar de S. Pedro el Viejo de Madrid ofrece, gracias a diversas intervenciones de época moderna y contemporánea, la posibilidad de analizar su desconocido proceso de construcción y sus técnicas constructivas donde se combinan fábricas de ladrillo y calicanto. Un muro de ladrillo con barro sirvió de encofrado de la fábrica de calicanto y luego fue utilizado como base de los primeros tramos de la escalera. También se utilizaron como encofrado la madera y los muros de ladrillo visto.

  9. Camilo Torres remembered on the eve of the fifty anniversary of his death

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    François Houtart

    2016-04-01

    Full Text Available A highly symbolic act took place in Cali on 7 November by initiative of Monsignor Dario Monsalve Jesus Mejia, archbishop of that city, the third of Colombia: The memory of Camilo Torres Restrepo as a Christian and as a priest. To prepare for the event, Archbishop had written in a magazine of the Archdiocese entitled I Camilo yesterday and today, a sign of reconciliation (Notebooks citizens - Observatory of social realities, number 5, November 2015, p. 8: “The priest Camilo Torres Restrepo, subject to their mortal remains to secret State.1 in the influence of his Christian thought, the silence of the Church and the guerrilla stigma on his name, has a lot to give and teach a Colombia that is projected towards reconciliation, the truth, transitional justice and peace

  10. Vanguardia, postvanguardia, posmodernidad. Max Aub, Jusep Torres Campalans y la vacunación vanguardista

    Directory of Open Access Journals (Sweden)

    Ette, Ottmar

    2002-12-01

    Full Text Available The analysis of one of Max Aub's most brilliant novels, «Jusep Torres Campalans» (1958, allows us a deep insight in the complex relationship between the «historic» Avantgarde and the Neoavantgardes of the sixties and seventies as well as between postvanguardist aesthetics and (the cultural space of Postmodernity. Focussing different forms of the break with the break of tradition, this essay deals with the avantgardist vaccination of postmodern cultural practices.

    Partiendo del análisis de una de las obras estéticamente más logradas de Max Aub, «Jusep Torres Campalans» (1958, el presente estudio enfoca las relaciones complejas entre las vanguardias «históricas» y las neovanguardias de los años sesenta y setenta, entre una estética postvanguardista y (el espacio cultural de la Posmodernidad, vacunada —en el sentido de Roland Barthes— con un poquito de Vanguardia.

  11. Isolation, Identification and Cytotoxicity of a New Noroleanane-Type Triterpene Saponin from Salicornia bigelovii Torr.

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    Fuqin Guan

    2015-04-01

    Full Text Available Salicornia bigelovii Torr. has been consumed not only as a popular kind of vegetable, but also as a medicinal plant to treat hypertension, cephalalgia, scurvy and cancer. The present study was designed to investigate its chemical components and cytotoxic activity. A new noroleanane-type triterpene saponin, bigelovii C (1, was separated and purified from Salicornia bigelovii Torr., along with four known triterpene saponins 2–5. The structure of bigelovii C was elucidated as 3-O-(6-O-butyl ester-β-D-glucuropyranosyl-23-aldehyde-30-norolean-12, 20 (29-dien-28-oic acid-28-O-β-D-glucopyranoside, according to various spectroscopic analysis and chemical characteristics. Besides Compounds 3 and 5, bigelovii C had potent cytotoxicity against three human cancer cell lines, MCF7 (breast cancer, Lovo (colon cancer and LN229 (glioblastoma, especially MCF7. Bigelovii C inhibited the growth of MCF7 cells in dose- and time-dependent manners. Flow cytometry analysis revealed that the percentage of apoptotic cells significantly increased upon bigelovii C treatment. Rh123 staining assay indicated that bigelovii C reduced the mitochondrial membrane potential. The mechanism of cell death by bigelovii C may be attributed to the downregulation of Bcl-2 and upregulation of Bax, cleaved caspase-9, caspase-7 and PARP. These results suggested that bigelovii C may impart health benefits when consumed and should be regarded as a potential chemopreventative agent for cancer.

  12. Genetic deficit of SK3 and IK1 channels disrupts the endothelium-derived hyperpolarizing factor vasodilator pathway and causes hypertension

    DEFF Research Database (Denmark)

    Brähler, Sebastian; Kaistha, Anuradha; Schmidt, Volker J;

    2009-01-01

    SK3 and IK1 in IK1(-/-)/SK3(T/T) mice was characterized by patch-clamp, membrane potential measurements, pressure myography, and intravital microscopy. Blood pressure was measured in conscious mice by telemetry. Combined IK1/SK3 deficiency in IK1(-/-)/SK3(T/T) (+doxycycline) mice abolished......-mediated vasodilation and lowered elevated blood pressure. The IK1-opener SKA-31 enhanced EDHF-mediated vasodilation and lowered blood pressure in SK3-deficient IK1(+/+)/SK3(T/T) (+doxycycline) mice to normotensive levels. CONCLUSIONS: Our study demonstrates that endothelial SK3 and IK1 channels have distinct stimulus...

  13. Is MuSK myasthenia gravis linked to IgG4-related disease?

    Science.gov (United States)

    Raibagkar, Pooja; Ferry, Judith A; Stone, John H

    2017-04-15

    Immunoglobulin (Ig) G4-related disease (IgG4-RD) is an immune-mediated inflammatory condition that affects a wide variety of sites, including the nervous system, where it can involve the meninges or the pituitary gland, and cause perineural mass lesions. A large subset of acetylcholine receptor antibody (Ab)-negative myasthenia gravis (MG) patients has muscle-specific tyrosine kinase (MuSK) Abs, generally of the IgG4 subclass. There has not been any association found between IgG4-RD and MuSK MG yet. We report the first case of MuSK MG associated with lymphadenopathy with histopathology consistent with IgG4-RD. A 54-year-old woman with MuSK MG developed eight compression fractures related to steroid therapy. Eighteen months after initial presentation she was found to have retroperitoneal lymphadenopathy with biopsy findings consistent with IgG4-RD. She was started on rituximab with clinical improvement and ability to taper immunomodulatory agents for the first time. Our case raises number of questions regarding a potential link between MuSK MG and IgG4-RD which may shed further light on the pathophysiology and management of these diseases. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Comparison of Visual, Refractive and Topographic Keratometry Outcomes of Intacs and Intacs SK in Mild to Moderate Keratoconus Eyes

    OpenAIRE

    Abdulrahman Al Muammar

    2015-01-01

    Purpose: The purpose was to evaluate and compare the visual and refractive outcomes, topographic keratometry (K) and complications of Intacs and Intacs SK for mild to moderate keratoconus. Methods: In this retrospective study, all mild to moderate keratoconus eyes that underwent implantation of Intacs (Intacs group) or Intacs SK (Intacs SK group) with minimum follow-up of 12 months were included. Preoperative and postoperative uncorrected distance visual acuity (UDVA), corrected distance ...

  15. Experimental Evaluation Of A Herbal Cream (SK-AC On Acetylated Lanolin Alcohol-Induced ACNE In Rabbit

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    Mitra S K

    1998-01-01

    Full Text Available Experimental studies were conducted in four groups of rabbits using external ear canal for inducing comedogenesis and its correction by a herbal formulation, viz., SK-AC cream. The result brought out that the SK-AC cream restricted the comedogenesis induced by acetylated lanolin alcohol when compared with other defined groups. Microcomedo being the basic pathology of acne, the outcome of this experiment pointed out the usefulness of SK-AC cream for the treatment of acne.

  16. La Huerta de Alicante y sus torres de defensa: propuesta de una ruta turística

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    Álvaro Francisco Morote Seguido

    2015-01-01

    Full Text Available La Huerta de Alicante y sus torres de defensa son un conjunto patrimonial que se podrían aprovechar para incorporar al mercado turístico de la ciudad de Alicante. Las torres de defensa son construcciones de los siglos XV-XVII que fueron utilizadas para que la población se refugiara ante los continuos ataques de piratas procedentes del Norte de África. En la actualidad, algunas de ellas han desaparecido o están en ruinas a pesar de estar declaradas Bien de Interés Cultural (BIC. El objetivo de esta investigación es promocionar la Huerta de Alicante y sus torres de defensa con la propuesta de una ruta turística.

  17. A propósito de torres y de libros: la circularidad de las lecturas (de Borges a Rapunzel

    Directory of Open Access Journals (Sweden)

    Eloy Martos

    2013-01-01

    Full Text Available La torre, la casa, el cuerpo, el mundo... son metáforas en suma del universo cuya correlación (en especial, cuerpo-casa-mundo ha puesto de evidencia M. Eliade. El libro, los libros que están en la torre del texto de Borges son en realidad las historias que pueblan nuestra imaginación, de ahí esa vinculación simbólica, que percibimos en los textos citados de Borges, en el poema de A. Machado, en el cuento de Rapunzel o en las leyendas de torres encantadas, igual que en las actuales narrativas transmediáticas tomadas como medios permeables con los clásicos y la cultura escrita en general.

  18. SkData: data sets and algorithm evaluation protocols in Python

    Science.gov (United States)

    Bergstra, James; Pinto, Nicolas; Cox, David D.

    2015-01-01

    Machine learning benchmark data sets come in all shapes and sizes, whereas classification algorithms assume sanitized input, such as (x, y) pairs with vector-valued input x and integer class label y. Researchers and practitioners know all too well how tedious it can be to get from the URL of a new data set to a NumPy ndarray suitable for e.g. pandas or sklearn. The SkData library handles that work for a growing number of benchmark data sets (small and large) so that one-off in-house scripts for downloading and parsing data sets can be replaced with library code that is reliable, community-tested, and documented. The SkData library also introduces an open-ended formalization of training and testing protocols that facilitates direct comparison with published research. This paper describes the usage and architecture of the SkData library.

  19. Um olhar sobre acervos escolares de Torres/RS – 1960/1980 = A look at collections schools of Torres/RS - 1960/1980

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    Eberhardt, Camila

    2014-01-01

    Full Text Available O artigo percorre o olhar sobre arquivos fotográficos de escolas da rede pública do município de Torres/RS entre os anos de 1960-1980. As instituições analisadas foram: o Instituto Estadual de Educação Marcílio Dias, a Escola Estadual de Ensino Fundamental Professor Justino Alberto Tietbohel e a Escola Estadual Governador Jorge Lacerda. Imagens que permitem identificar quais foram às opções iconográficas das instituições de ensino. Entre quatrocentas e quarenta e seis fotografias que foram digitalizadas, medidas, catalogadas e identificadas treze temáticas visuais. Destacaram-se imagens que podem ser enquadradas ao âmbito do extraordinário, ou seja, imagens que registraram os momentos festivos, os passeios, as comemorações cívicas, as formaturas, etc. , a opção por essas temáticas constatou-se serem decorrentes em grande medida do acesso restrito da câmera fotográfica, e ao que diz respeito ao ordinário, o registro ocorreu, porém, em pequena proporção. Por meio desses registros iconográficos pretende-se discutir a importância e o uso dado as fotografias nas instituições de ensino. Visto que as fotografias foram encontradas em péssimo estado de conservação

  20. Mitochondria-Targeted Antioxidant SkQ1 Improves Dermal Wound Healing in Genetically Diabetic Mice

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    Ilya A. Demyanenko

    2017-01-01

    Full Text Available Oxidative stress is widely recognized as an important factor in the delayed wound healing in diabetes. However, the role of mitochondrial reactive oxygen species in this process is unknown. It was assumed that mitochondrial reactive oxygen species are involved in many wound-healing processes in both diabetic humans and animals. We have applied the mitochondria-targeted antioxidant 10-(6′-plastoquinonyldecyltriphenylphosphonium (SkQ1 to explore the role of mitochondrial reactive oxygen species in the wound healing of genetically diabetic mice. Healing of full-thickness excisional dermal wounds in diabetic C57BL/KsJ-db−/db− mice was significantly enhanced after long-term (12 weeks administration of SkQ1. SkQ1 accelerated wound closure and stimulated epithelization, granulation tissue formation, and vascularization. On the 7th day after wounding, SkQ1 treatment increased the number of α-smooth muscle actin-positive cells (myofibroblasts, reduced the number of neutrophils, and increased macrophage infiltration. SkQ1 lowered lipid peroxidation level but did not change the level of the circulatory IL-6 and TNF. SkQ1 pretreatment also stimulated cell migration in a scratch-wound assay in vitro under hyperglycemic condition. Thus, a mitochondria-targeted antioxidant normalized both inflammatory and regenerative phases of wound healing in diabetic mice. Our results pointed to nearly all the major steps of wound healing as the target of excessive mitochondrial reactive oxygen species production in type II diabetes.

  1. Dexamethasone blocks the migration of the human neuroblastoma cell line SK-N-SH

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    L.A. Casulari

    2006-09-01

    Full Text Available Glucocorticoids (Gc influence the differentiation of neural crest-derived cells such as those composing sympathoadrenal tumors like pheochromocytomas, as well as neuroblastomas and gangliomas. In order to obtain further information on the effects of Gc on cells evolving from the neural crest, we have used the human neuroblastoma cell line SK-N-SH to analyze: 1 the presence and the binding characteristics of Gc receptors in these cells, 2 the effect of dexamethasone (Dex on the migration of SK-N-SH cells, and 3 the effect of Dex on the organization of the cytoskeleton of SK-N-SH cells. We show that: 1 receptors that bind [³H]-Dex with high affinity and high capacity (Kd of 9.6 nM, Bmax of 47 fmol/mg cytosolic protein, corresponding to 28,303 sites/cell are present in cytosolic preparations of SK-N-SH cells, and 2 treatment with Dex (in the range of 10 nM to 1 µM has an inhibitory effect (from 100% to 74 and 43%, respectively on the chemotaxis of SK-N-SH cells elicited by fetal bovine serum. This inhibition is completely reversed by the Gc receptor antagonist RU486 (1 µM, and 3 as demonstrated by fluorescent phalloidin-actin detection, the effect of Dex (100 nM on SK-N-SH cell migration is accompanied by modifications of the cytoskeleton organization that appear with stress fibers. These modifications did not take place in the presence of 1 µM RU486. The present data demonstrate for the first time that Dex affects the migration of neuroblastoma cells as well as their cytoskeleton organization by interacting with specific receptors. These findings provide new insights on the mechanism(s of action of Gc on cells originating in the neural crest.

  2. Dexamethasone blocks the migration of the human neuroblastoma cell line SK-N-SH

    Directory of Open Access Journals (Sweden)

    L.A. Casulari

    Full Text Available Glucocorticoids (Gc influence the differentiation of neural crest-derived cells such as those composing sympathoadrenal tumors like pheochromocytomas, as well as neuroblastomas and gangliomas. In order to obtain further information on the effects of Gc on cells evolving from the neural crest, we have used the human neuroblastoma cell line SK-N-SH to analyze: 1 the presence and the binding characteristics of Gc receptors in these cells, 2 the effect of dexamethasone (Dex on the migration of SK-N-SH cells, and 3 the effect of Dex on the organization of the cytoskeleton of SK-N-SH cells. We show that: 1 receptors that bind [³H]-Dex with high affinity and high capacity (Kd of 9.6 nM, Bmax of 47 fmol/mg cytosolic protein, corresponding to 28,303 sites/cell are present in cytosolic preparations of SK-N-SH cells, and 2 treatment with Dex (in the range of 10 nM to 1 µM has an inhibitory effect (from 100% to 74 and 43%, respectively on the chemotaxis of SK-N-SH cells elicited by fetal bovine serum. This inhibition is completely reversed by the Gc receptor antagonist RU486 (1 µM, and 3 as demonstrated by fluorescent phalloidin-actin detection, the effect of Dex (100 nM on SK-N-SH cell migration is accompanied by modifications of the cytoskeleton organization that appear with stress fibers. These modifications did not take place in the presence of 1 µM RU486. The present data demonstrate for the first time that Dex affects the migration of neuroblastoma cells as well as their cytoskeleton organization by interacting with specific receptors. These findings provide new insights on the mechanism(s of action of Gc on cells originating in the neural crest.

  3. In vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman.

    Science.gov (United States)

    Kaewnopparat, Sanae; Dangmanee, Nattakan; Kaewnopparat, Nattha; Srichana, Teerapol; Chulasiri, Malyn; Settharaksa, Sukanya

    2013-08-01

    A lactobacillus strain isolated from a vaginal tract of a healthy woman was examined in vitro for its probiotic potential. This strain, identified as Lactobacillus fermentum SK5, was able to survive at pH 3-4 and 0.1-0.2% bile, and unaffected by pepsin (3 g l(-1)) and pancreatin (1 g l(-1)), but was susceptible to all tested antibiotics except metronidazole. L. fermentum SK5 had an antimicrobial potential against gastrointestinal pathogenic Escherichia coli and vaginal pathogenic Gardnerella vaginalis. The effective substance was suspected to be a bacteriocin-like compound with a molecular weight of more than 10 kDa, but hydrogen peroxide was also detected. Further studies revealed that L. fermentum SK5 had good autoaggregation characteristic and a high surface hydrophobicity that enhanced its adhesion ability to epithelial cells and for biofilm formation. This lactobacillus showed coaggregation with E. coli and G. vaginalis to affect their adhesion and colonization. The adhesion of L. fermentum SK5 to HeLa, HT-29 and Caco-2 cells and its inhibition of E. coli and G. vaginalis adherence to these cells were demonstrated. These incidences provided evidence of the possible colonization of L. fermentum SK5 that would prevent binding and growth of E. coli and G. vaginalis onto intestinal and vaginal epithelial cells. On the basis of the ability of L. fermentum SK5 to inhibit pathogenic microorganisms through coaggregation and antimicrobial substances, it is likely that this lactobacillus strain could be a potential probiotic candidate for beneficial use in protecting against gastrointestinal and vaginal microbial infections. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Impacto ambiental de las torres de alta tensión en el paisaje urbano de Santiago de Chile

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    M. Salinas Torres

    2008-01-01

    a países Iberoamericanos. La investigación consiste en la valoración ambiental del impacto ambiental y visual que causan las torres de alta tensión en Santiago de Chile. Para ello se diseñan extensas matrices cuyos resultados mediante SIG se traducen en una cartografía específica y de síntesis. La propuesta final es reutilizar el territorio que ocupan las torres en la ciudad una vez soterradas las catenarias, de esta forma se generarían áreas verdes de alto valor ambiental.

  5. El arquitecto madrileño Pedro de la Torre en Toledo y un retablo inédito localizado

    OpenAIRE

    Antonio José Díaz Fernández

    2013-01-01

    Entre las realizaciones del arquitecto barroco Pedro de la Torre para la ciudad de Toledo es posible identificar un retablo hasta ahora inédito, datado en 1653 y en el que colabora el ensamblador toledano Juan García de San Pedro, localizado hoy en la parroquia de Nambroca (Toledo), en el que se aprecian las innovaciones estructurales y ornamentales propias del lenguaje desarrollado por el artista.Among the works of the baroque architect Pedro de la Torre for the city of Toledo is possible to...

  6. High risk alcohol-related trauma among the Aboriginal and Torres Strait Islanders in the Northern Territory.

    Science.gov (United States)

    Jayaraj, Rama; Thomas, Mahiban; Thomson, Valerie; Griffin, Carolyn; Mayo, Luke; Whitty, Megan; d'Abbs, Peter; Nagel, Tricia

    2012-08-03

    High risk drinking is linked with high rates of physical harm. The reported incidence of alcohol - related trauma among Aboriginal and Torres Strait Islander people in the Northern Territory is the highest in the world. Facial fractures are common among young Aboriginal and Torres Strait Islanders. They are often linked with misuse of alcohol in the Northern Territory and are frequently secondary to assault. This review focuses on alcohol-related trauma in the Territory and draws attention to an urgent need for preventative health approach to address this critical issue.

  7. Anti-MuSK-Positive Myasthenia Gravis in a Patient with Parkinsonism and Cognitive Impairment

    Directory of Open Access Journals (Sweden)

    S. Lanfranconi

    2011-01-01

    Full Text Available Muscle-specific tyrosine kinase- (MuSK- antibodies-positive Myasthenia Gravis accounts for about one third of Seronegative Myasthenia Gravis and is clinically characterized by early onset of prominent bulbar, neck, shoulder girdle, and respiratory weakness. The response to medical therapy is generally poor. Here we report a case of late-onset MuSK-antibodies-positive Myasthenia Gravis presenting with signs of cognitive impairment and parkinsonism in addition to bulbar involvement and external ophthalmoplegia. The pattern of involvement of both peripheral and central nervous system dysfunction might suggest a common pathogenic mechanism, involving impaired cholinergic transmission.

  8. Appropriate Health Promotion for Australian Aboriginal and Torres Strait Islander Communities

    DEFF Research Database (Denmark)

    Demaio, Alessandro Rhyll; Drysdale, Marlene; de Courten, Maximilian

    2012-01-01

    Health promotion for Australian Aboriginal and Torres Strait Islander communities and their people has generally had limited efficacy and poor sustainability. It has largely failed to recognise and appreciate the importance of local cultures and continues to have minimal emphasis on capacity...... building, community empowerment and local ownership. Culturally-Appropriate Health Promotion is a framework of principles developed in 2008 with the World Health Organization (Geneva) and Global Alliance for Health Promotion. It guides community-focused health promotion practice built on and shaped...... by the respect, understanding and utilisation of local knowledge and culture. Culturally-Appropriate Health Promotion is not about ‘targeting’, ‘intervening’ or ‘responding’. Rather, it results in health program planners and policy-makers understanding, respecting, empowering and collaborating with communities...

  9. O mito da cidade moderna e a arte: Torres-García e Xul Solar

    Directory of Open Access Journals (Sweden)

    Kern, Maria Lúcia Bastos

    2004-01-01

    Full Text Available A cidade se constitui como o espaço privilegiado para criar a estética da modernidade, visto que ela proporciona novas percepções a respeito das noções de espaço e tempo, permitindo aos artistas absorverem no seu fluxo incessante de mudanças os signos dos novos tempos. O presente artigo tem o fim de analisar as obras de dois artistas modernos platinos - Joaquín Torres-García e Xul Solar - e verificar como a cidade é utilizada para a elaboração das suas poéticas e é concebida e mitificada

  10. Desenvolvimento de heliostato para geração heliotermica em torres solares

    OpenAIRE

    Silvestre, Alysson Domingos

    2016-01-01

    Com o aumento das perspectivas mundiais a respeito das energias renováveis e a utilização da energia solar térmica concentrada, com o objetivo de aumentar o fluxo de radiação solar incidente em sistemas térmicos tem se mostrado uma solução viável para geração de energia elétrica. Os dispositivos heliostatos tem a capacidade de rastrear o sol e refletir a radiação em um ponto fixo pré – estabelecido, normalmente conhecido como torre solar localizando se ao meio de um campo de dispositivos heli...

  11. A shark in the stars: astronomy and culture in the Torres Strait

    CERN Document Server

    Hamacher, Duane W

    2014-01-01

    Technology has, without doubt, expanded our understanding of space. The Voyager 1 space probe is on the brink of leaving our solar system. Massive telescopes have discovered blasts of fast radio bursts from 10 billion light years away. And after a decade on Mars, a Rover recently found evidence for an early ocean on the Red Planet. But with every new advance, it's also important to remember the science of astronomy has existed for thousands of years and forms a vital part of Indigenous Australian culture, even today. As an example, let's explore the astronomy of the Torres Strait Islanders, an Indigenous Australian people living between the tip of Cape York and Papua New Guinea.

  12. Ciencia, revolución y creencia en Camilo Torres: ¿una Colombia secular?

    Directory of Open Access Journals (Sweden)

    Alejandro Sánchez Lopera

    2006-10-01

    Full Text Available Camilo Torres (1929-1966, presentado en la literatura como “científico”, fundador del primer programa disciplinar de sociología en América Latina; como ���revolucionario”, combatiente de la guerrilla foquista del Ejército de Liberación Nacional (ELN; como “sacerdote”, figura decisiva en la dinámica de los movimientos eclesiales contestatarios de América Latina y Centroamérica. Este artículo analiza la forma en que, de acuerdo con la literatura que lo construye como personaje, su experiencia alteró las prácticas científicas, transformó los modos de lucha revolucionarios, y reconfiguró las articulaciones entre creencia y política.

  13. Removal of lead ions from aqueous solution by the dried aquatic plant, Lemna perpusilla Torr

    Energy Technology Data Exchange (ETDEWEB)

    Tang, Yankui, E-mail: tangyankui101@163.com [College of Environment, Guangxi University, Nanning 530004 (China); Chen, Ling; Wei, Xingren; Yao, Qiuyan; Li, Ting [College of Environment, Guangxi University, Nanning 530004 (China)

    2013-01-15

    Highlights: ► We found dried Lemna perpusilla Torr. acted as a respective adsorbent for Pb removal. ► We found the adsorption isotherm followed Langmuir model. ► We found the adsorption kinetics followed the pseudo-second-order model. ► We found Pb(II) adsorption on the biomass could be physical and chemical. -- Abstract: The aquatic plant, Lemna perpusilla Torr. strain, was dried, pulverized and used for Pb(II) removal from aqueous solution. Batch experiments were conducted to investigate the effects of pH, contact time, initial metal concentration and temperature on Pb(II) adsorption. A dose of 4 g L{sup −1} of dried L. perpusilla in a solution with an initial pH of 4.6, an initial Pb(II) concentration of 50 mg L{sup −1} and a contact time of 210 min resulted in the maximum Pb(II) removal efficiency (above 95%). The equilibrium adsorption capacities increased with increasing initial Pb(II) concentration. The adsorption isotherm was better described by a Langmuir model rather than a Freundlich model. Further, the adsorption kinetics followed a pseudo-second-order model. An FTIR examination revealed changes between the natural and Pb(II)-loaded plant material. Scanning electron micrograph (SEM) also revealed changes in the surface morphology of the biomass as a result of lead adsorption. Based on these results, it can be concluded that the dried L. perpusilla is effective in removing lead from aqueous solution and merits consideration for scaled-up trials.

  14. Seagrass-Watch: Engaging Torres Strait Islanders in marine habitat monitoring

    Science.gov (United States)

    Mellors, Jane E.; McKenzie, Len J.; Coles, Robert G.

    2008-09-01

    Involvement in scientifically structured habitat monitoring is a relatively new concept to the peoples of Torres Strait. The approach we used was to focus on awareness, and to build the capacity of groups to participate using Seagrass-Watch as the vehicle to provide education and training in monitoring marine ecosystems. The project successfully delivered quality scientifically rigorous baseline information on the seasonality of seagrasses in the Torres Strait—a first for this region. Eight seagrass species were identified across the monitoring sites. Seagrass cover varied within and between years. Preliminary evidence indicated that drivers for seagrass variability were climate related. Generally, seagrass abundance increased during the north-west monsoon ( Kuki), possibly a consequence of elevated nutrients, lower tidal exposure times, less wind, and higher air temperatures. Low seagrass abundance coincided with the presence of greater winds and longer periods of exposure at low tides during the south-east trade wind season ( Sager). No seasonal patterns were apparent when frequency of disturbance from high sedimentation and human impacts was high. Seagrass-Watch has been incorporated in to the Thursday Island High School's Marine Studies Unit ensuring continuity of monitoring. The students, teachers, and other interested individuals involved in Seagrass-Watch have mastered the necessary scientific procedures to monitor seagrass meadows, and developed skills in coordinating a monitoring program and skills in mentoring younger students. This has increased the participants' self-esteem and confidence, and given them an insight into how they may participate in the future management of their sea country.

  15. The potential impact of bedform migration on seagrass communities in Torres Strait, northern Australia

    Science.gov (United States)

    Daniell, James J.; Harris, Peter T.; Hughes, Michael G.; Hemer, Mark; Heap, Andrew

    2008-09-01

    Seagrass communities in the northwest of Torres Strait are known to disappear episodically over broad areas. Sediment mobility surveys were undertaken within two study areas during the monsoon and trade wind seasons, in the vicinity of Turnagain Island, to find out if the migration of bedforms could explain this disappearance. The two study areas covered sand bank and sand dune environments to compare and contrast their migration characteristics. Repeat multibeam sonar surveys were used to measure dune-crest migration during each season. Our results show that seagrass beds occur in the troughs of sediment-starved dunes, but no seagrass occurs in association with full-bedded dunes that are superimposed on large sand bank features. The coincidence of seagrass beds with the sediment-starved dunes is in spite of the fact that they migrate faster (0.59 m day -1) than full-bedded dunes (0.13 m day -1), which indicates that some other factor (other than dune migration rate) limits seagrass growth within Torres Strait. We suggest that seagrasses are unable to colonise full-bedded dunes because of the semi-continuously transported sand that characterises this environment. In contrast, the troughs of sediment-starved dunes experience only limited bedload transport and are less hostile for seagrasses. A conceptual model is presented to explain the occurrence of seagrass beds in relation to their proximity to migrating sand dunes. Based on our analysis, we conclude that the widespread dieback of seagrasses documented for the Turnagain Island region was not caused by dune migration.

  16. Cloning and characterization of SK2 channel from chicken short hair cells.

    Science.gov (United States)

    Matthews, T M; Duncan, R K; Zidanic, M; Michael, T H; Fuchs, P A

    2005-06-01

    In the inner ear of birds, as in mammals, reptiles and amphibians, acetylcholine released from efferent neurons inhibits hair cells via activation of an apamin-sensitive, calcium-dependent potassium current. The particular potassium channel involved in avian hair cell inhibition is unknown. In this study, we cloned a small-conductance, calcium-sensitive potassium channel (gSK2) from a chicken cochlear library. Using RT-PCR, we demonstrated the presence of gSK2 mRNA in cochlear hair cells. Electrophysiological studies on transfected HEK293 cells showed that gSK2 channels have a conductance of approximately 16 pS and a half-maximal calcium activation concentration of 0.74+/-0.17 microM. The expressed channels were blocked by apamin (IC(50)=73.3+/-5.0 pM) and d-tubocurarine (IC(50)=7.6+/-1.0 microM), but were insensitive to charybdotoxin. These characteristics are consistent with those reported for acetylcholine-induced potassium currents of isolated chicken hair cells, suggesting that gSK2 is involved in efferent inhibition of chicken inner ear. These findings imply that the molecular mechanisms of inhibition are conserved in hair cells of all vertebrates.

  17. New metabolic activity of the nonsulfated sulfakinin Zopat-SK-1 in the insect fat body.

    Science.gov (United States)

    Slocinska, Malgorzata; Marciniak, Pawel; Jarmuszkiewicz, Wieslawa; Rosinski, Grzegorz

    2015-06-01

    Insect sulfakinins are multifunctional neuropeptides homologous to vertebrate gastrin/cholecystokin (CCK) neuropeptide hormones. We investigated the action of the nonsulfated sulfakinin Zopat-SK-1 (pETSDDYGHLRFa) on the levels of chosen metabolites in the Zophobas atratus beetle fat body. Samples of fat body were collected 2h and 24h after hormone injection. The administration of 20pmol of Zopat-SK-1 to feeding larvae significantly increased concentrations of lipids and proteins and decreased the content of glycogen in fat body tissue in the 24h experimental group. In contrast, the only increase in total lipid concentration in prepupal fat bodies was observed 24h after Zopat-SK-1 treatment. Simultaneously, changes in the quality and quantity of free sugars in the hemolymph were measured. In larval hemolymph, a marked increase in free sugar concentration and a decrease in glucose content were observed 24h and 2h after Zopat-SK-1 application, respectively. No changes in the prepupal stage were observed. For the first time we show potent metabolic activity of sulfakinin in the fat body tissue of an insect. Our findings imply a physiological function of the nonsulfated form of sulfakinin in energy storage and release processes in fat body tissue of larvae and prepupae was indicated. We suggest a role for sulfakinin signaling in the regulation of energy metabolism in insect tissues. Copyright © 2014 Elsevier Inc. All rights reserved.

  18. 7SK small nuclear RNA inhibits cancer cell proliferation through apoptosis induction.

    Science.gov (United States)

    Keramati, Farid; Seyedjafari, Ehsan; Fallah, Parviz; Soleimani, Masoud; Ghanbarian, Hossein

    2015-04-01

    7SK small nuclear RNA (snRNA) is a 331-333-bp non-coding RNA, which recruits HEXIM 1/2 protein to inhibit positive elongation factor b (P-TEFb) activity. P-TEFb is an essential factor in alleviating promoter-proximal paused RNA polymerase II (Pol II) and initiating the productive elongation phase of gene transcription. Without this protein, Pol II will remain in its hypophosphorylated state, and no transcription occurs. In this study, we inhibited P-TEFb activity by over-expressing 7SK snRNA in human embryonic kidney (HEK) 293T cancer cell line. This inhibition led to a significant decrease in cell viability, which can be due to the transcription inhibition. Moreover, 7SK snRNA over-expression promoted apoptosis in cancerous cells. Our results suggest 7SK snRNA as a potential endogenous anti-cancer agent, and to the best of our knowledge, this is the first study that uses a long non-coding RNA's over-expression against cancer cell growth and proliferation.

  19. SK channels mediate NADPH oxidase-independent reactive oxygen species production and apoptosis in granulocytes.

    Science.gov (United States)

    Fay, Alex J; Qian, Xiang; Jan, Yuh Nung; Jan, Lily Yeh

    2006-11-14

    Neutrophils are immune cells that bind to, engulf, and destroy bacterial and fungal pathogens in infected tissue, and their clearance by apoptosis is essential for the resolution of inflammation. Killing involves both oxidative and nonoxidative processes, the oxidative pathway requiring electrogenic production of superoxide by the membrane-bound NADPH oxidase complex. A variety of stimuli, from bacterial chemotactic peptides to complement- or IgG-opsonized microbes, can induce the production of reactive oxygen species (ROS) by neutrophils, presumably by means of NADPH oxidase. We report here that 1-ethyl-2-benzimidazolinone (1-EBIO), an activator of Ca2+-activated potassium channels of small conductance (SK) and intermediate conductance (IK), causes production of superoxide and hydrogen peroxide by neutrophils and granulocyte-differentiated PLB-985 cells. This response can be partially inhibited by the SK blocker apamin, which inhibits a Ca2+-activated K+ current in these cells. Analysis of RNA transcripts indicates that channels encoded by the SK3 gene carry this current. The effects of 1-EBIO and apamin are independent of the NADPH oxidase pathway, as demonstrated by using a PLB-985 cell line lacking the gp91phox subunit. Rather, 1-EBIO and apamin modulate mitochondrial ROS production. Consistent with the enhanced ROS production and K+ efflux mediated by 1-EBIO, we found that this SK opener increased apoptosis of PLB-985 cells. Together, these findings suggest a previously uncharacterized mechanism for the regulation of neutrophil ROS production and programmed cell death.

  20. Hvem sidder dér bag skærmen?

    DEFF Research Database (Denmark)

    Werlauff, Erik; Schaumburg-Müller, Peer

    2009-01-01

    Med udgangspunkt i nutidens daytrader, der sidder ´"bag skærmen" og foretager talrige daglige køb og salg af børsnoterede aktier mv., analyseres det i artiklen, hvornår en sådan lovlig daytrading udarter til strafbar kursmanipulation. Nogle daytradere har ment, at hvis en ikke-professionel invest...

  1. Bifurcation diagram globally underpinning neuronal firing behaviors modified by SK conductance

    Science.gov (United States)

    Chen, Meng-Jiao; Ling, Heng-Li; Liu, Yi-Hui; Qu, Shi-Xian; Ren, Wei

    2014-02-01

    Neurons in the brain utilize various firing trains to encode the input signals they have received. Firing behavior of one single neuron is thoroughly explained by using a bifurcation diagram from polarized resting to firing, and then to depolarized resting. This explanation provides an important theoretical principle for understanding neuronal biophysical behaviors. This paper reports the novel experimental and modeling results of the modification of such a bifurcation diagram by adjusting small conductance potassium (SK) channel. In experiments, changes in excitability and depolarization block in nucleus accumbens shell and medium-spiny projection neurons are explored by increasing the intensity of injected current and blocking the SK channels by apamin. A shift of bifurcation points is observed. Then, a Hodgkin—Huxley type model including the main electrophysiological processes of such neurons is developed to reproduce the experimental results. The reduction of SK channel conductance also shifts the bifurcations, which is in consistence with experiment. A global bifurcation paradigm of this shift is obtained by adjusting two parameters, intensity of injected current and SK channel conductance. This work reveals the dynamics underpinning modulation of neuronal firing behaviors by biologically important ionic conductance. The results indicate that small ionic conductance other than that responsible for spike generation can modify bifurcation points and shift the bifurcation diagram and, thus, change neuronal excitability and adaptation.

  2. Control of Ca2+ Influx and Calmodulin Activation by SK-Channels in Dendritic Spines.

    Directory of Open Access Journals (Sweden)

    Thom Griffith

    2016-05-01

    Full Text Available The key trigger for Hebbian synaptic plasticity is influx of Ca2+ into postsynaptic dendritic spines. The magnitude of [Ca2+] increase caused by NMDA-receptor (NMDAR and voltage-gated Ca2+ -channel (VGCC activation is thought to determine both the amplitude and direction of synaptic plasticity by differential activation of Ca2+ -sensitive enzymes such as calmodulin. Ca2+ influx is negatively regulated by Ca2+ -activated K+ channels (SK-channels which are in turn inhibited by neuromodulators such as acetylcholine. However, the precise mechanisms by which SK-channels control the induction of synaptic plasticity remain unclear. Using a 3-dimensional model of Ca2+ and calmodulin dynamics within an idealised, but biophysically-plausible, dendritic spine, we show that SK-channels regulate calmodulin activation specifically during neuron-firing patterns associated with induction of spike timing-dependent plasticity. SK-channel activation and the subsequent reduction in Ca2+ influx through NMDARs and L-type VGCCs results in an order of magnitude decrease in calmodulin (CaM activation, providing a mechanism for the effective gating of synaptic plasticity induction. This provides a common mechanism for the regulation of synaptic plasticity by neuromodulators.

  3. Tee Toompealt Pääskülani ja Tallinna saras / Tõnu Raid

    Index Scriptorium Estoniae

    Raid, Tõnu, 1939-

    2009-01-01

    Keskaegses Tallinnas puudus maamõõtja. Esimese säilinud Tallinna linnaplaani valmistas 1680. aastal Tallinna gümnaasiumi professor Heinrich Julius Woltemate. 1807. aastal loodi linna maamõõtja koht. Kõrgepea nuki asukohast. Pärnu maanteest esimestel kaartidel. Pärnu maantee üleviimisest. Muinasaja teest Pääskülast Tallinna.

  4. SK2 channel modulation contributes to compartment-specific dendritic plasticity in cerebellar Purkinje cells.

    Science.gov (United States)

    Ohtsuki, Gen; Piochon, Claire; Adelman, John P; Hansel, Christian

    2012-07-12

    Small-conductance Ca(2+)-activated K(+) channels (SK channels) modulate excitability and curtail excitatory postsynaptic potentials (EPSPs) in neuronal dendrites. Here, we demonstrate long-lasting plasticity of intrinsic excitability (IE) in dendrites that results from changes in the gain of this regulatory mechanism. Using dendritic patch-clamp recordings from rat cerebellar Purkinje cells, we find that somatic depolarization or parallel fiber (PF) burst stimulation induce long-term amplification of synaptic responses to climbing fiber (CF) or PF stimulation and enhance the amplitude of passively propagated sodium spikes. Dendritic plasticity is mimicked and occluded by the SK channel blocker apamin and is absent in Purkinje cells from SK2 null mice. Triple-patch recordings from two dendritic sites and the soma and confocal calcium imaging studies show that local stimulation limits dendritic plasticity to the activated compartment of the dendrite. This plasticity mechanism allows Purkinje cells to adjust the SK2-mediated control of dendritic excitability in an activity-dependent manner.

  5. Mobile Devices for Tertiary Study--Philosophy Meets Pragmatics for Remote Aboriginal and Torres Strait Islander Women

    Science.gov (United States)

    Townsend, Philip

    2015-01-01

    This paper outlines PhD research which suggests mobile learning fits the cultural philosophies and roles of Aboriginal and Torres Strait Islander women who are preservice teachers in the very remote Australian communities where the research was conducted. The problem which the research addresses is the low completion rates for two community-based…

  6. The Fallacy of the Bolted Horse: Changing Our Thinking about Mature-Age Aboriginal and Torres Strait Islander University Students

    Science.gov (United States)

    Plater, Suzanne; Mooney-Somers, Julie; Lander, Jo

    2015-01-01

    The aim of this article is to critically review and analyse the public representations of mature-age university students in developed and some developing nations and how they compare to the public representations of mature-age Aboriginal and Torres Strait Islander university students in Australia ("students" also refers to graduates…

  7. Everywhere and Nowhere: Invisibility of Aboriginal and Torres Strain Islander Contact Languages in Education and Indigenous Language Contexts

    Science.gov (United States)

    Sellwood, Juanita; Angelo, Denise

    2013-01-01

    The language ecologies of Aboriginal and Torres Strait Islander communities in Queensland are characterised by widespread language shift to contact language varieties, yet they remain largely invisible in discourses involving Indigenous languages and education. This invisibility--its various causes and its many implications--are explored through a…

  8. Hearing the Voice of Remote Aboriginal and Torres Strait Islander Training Stakeholders Using Research Methodologies and Theoretical Frames of Reference

    Science.gov (United States)

    Guenther, John; Osborne, Sam; Arnott, Allan; McRae-Williams, Eva

    2017-01-01

    Researchers in remote Aboriginal and Torres Strait Islander contexts within Australia are frequently faced with the challenges of working in an intercultural space where channels of communication are garbled with interference created by the complexities of misunderstood worldviews, languages, values and expectations. A concern of many researchers…

  9. Acquired resistance to oxaliplatin is not directly associated with increased resistance to DNA damage in SK-N-ASrOXALI4000, a newly established oxaliplatin-resistant sub-line of the neuroblastoma cell line SK-N-AS

    Science.gov (United States)

    Saintas, Emily; Abrahams, Liam; Ahmad, Gulshan T.; Ajakaiye, Anu-Oluwa M.; AlHumaidi, Abdulaziz S. H. A. M.; Ashmore-Harris, Candice; Clark, Iain; Dura, Usha K.; Fixmer, Carine N.; Ike-Morris, Chinedu; Mato Prado, Mireia; Mccullough, Danielle; Mishra, Shishir; Schöler, Katia M. U.; Timur, Husne; Williamson, Maxwell D. C.; Alatsatianos, Markella; Bahsoun, Basma; Blackburn, Edith; Hogwood, Catherine E.; Lithgow, Pamela E.; Rowe, Michelle; Yiangou, Lyto; Rothweiler, Florian; Cinatl, Jindrich; Zehner, Richard; Baines, Anthony J.; Garrett, Michelle D.; Gourlay, Campbell W.; Griffin, Darren K.; Gullick, William J.; Hargreaves, Emma; Howard, Mark J.; Lloyd, Daniel R.; Rossman, Jeremy S.; Smales, C. Mark; Tsaousis, Anastasios D.; von der Haar, Tobias; Wass, Mark N.

    2017-01-01

    The formation of acquired drug resistance is a major reason for the failure of anti-cancer therapies after initial response. Here, we introduce a novel model of acquired oxaliplatin resistance, a sub-line of the non-MYCN-amplified neuroblastoma cell line SK-N-AS that was adapted to growth in the presence of 4000 ng/mL oxaliplatin (SK-N-ASrOXALI4000). SK-N-ASrOXALI4000 cells displayed enhanced chromosomal aberrations compared to SK-N-AS, as indicated by 24-chromosome fluorescence in situ hybridisation. Moreover, SK-N-ASrOXALI4000 cells were resistant not only to oxaliplatin but also to the two other commonly used anti-cancer platinum agents cisplatin and carboplatin. SK-N-ASrOXALI4000 cells exhibited a stable resistance phenotype that was not affected by culturing the cells for 10 weeks in the absence of oxaliplatin. Interestingly, SK-N-ASrOXALI4000 cells showed no cross resistance to gemcitabine and increased sensitivity to doxorubicin and UVC radiation, alternative treatments that like platinum drugs target DNA integrity. Notably, UVC-induced DNA damage is thought to be predominantly repaired by nucleotide excision repair and nucleotide excision repair has been described as the main oxaliplatin-induced DNA damage repair system. SK-N-ASrOXALI4000 cells were also more sensitive to lysis by influenza A virus, a candidate for oncolytic therapy, than SK-N-AS cells. In conclusion, we introduce a novel oxaliplatin resistance model. The oxaliplatin resistance mechanisms in SK-N-ASrOXALI4000 cells appear to be complex and not to directly depend on enhanced DNA repair capacity. Models of oxaliplatin resistance are of particular relevance since research on platinum drugs has so far predominantly focused on cisplatin and carboplatin. PMID:28192521

  10. Survivin selective inhibitor YM155 induce apoptosis in SK-NEP-1 Wilms tumor cells

    Directory of Open Access Journals (Sweden)

    Tao Yan-Fang

    2012-12-01

    Full Text Available Abstract Background Survivin, a member of the family of inhibitor of apoptosis proteins, functions as a key regulator of mitosis and programmed cell death. YM155, a novel molecular targeted agent, suppresses survivin, which is overexpressed in many tumor types. The aim of this study was to determine the antitumor activity of YM155 in SK-NEP-1 cells. Methods SK-NEP-1 cell growth in vitro and in vivo was assessed by MTT and nude mice experiments. Annexin V/propidium iodide staining followed by flow cytometric analysis was used to detect apoptosis in cell culture. Then gene expression profile of tumor cells treated with YM155 was analyzed with real-time PCR arrays. We then analyzed the expression data with MEV (Multi Experiment View cluster software. Datasets representing genes with altered expression profile derived from cluster analyses were imported into the Ingenuity Pathway Analysis tool. Results YM155 treatment resulted in inhibition of cell proliferation of SK-NEP-1cells in a dose-dependent manner. Annexin V assay, cell cycle, and activation of caspase-3 demonstrates that YM155 induced apoptosis in SK-NEP-1 cells. YM155 significantly inhibited growth of SK-NEP-1 xenografts (YM155 5 mg/kg: 1.45 ± 0.77 cm3; YM155 10 mg/kg: 0.95 ± 0.55 cm3 compared to DMSO group (DMSO: 3.70 ± 2.4 cm3 or PBS group cells (PBS: 3.78 ± 2.20 cm3, ANOVA P Conclusions The present study demonstrates that YM155 treatment resulted in apoptosis and inhibition of cell proliferation of SK-NEP-1cells. YM155 had significant role and little side effect in the treatment of SK-NEP-1 xenograft tumors. Real-time PCR array analysis firstly showed expression profile of genes dyes-regulated after YM155 treatment. IPA analysis also represents new molecule mechanism of YM155 treatment, such as NR3C1 and dexamethasone may be new target of YM155. And our results may provide new clues of molecular mechanism of apoptosis induced by YM155.

  11. Skórna postać leiszmaniozy u pacjenta powracającego z Maroka

    Directory of Open Access Journals (Sweden)

    Aleksander Garlicki

    2010-09-01

    Full Text Available Wprowadzenie: Leiszmanioza jest tropikalną chorobą pasożytniczą,wywołaną przez pierwotniaki z rodzaju Leishmania. RezerwuaremLeishmania są ludzie i zwierzęta, a wektorem przenoszącym pasożytakomary z rodzaju Phlebotomus i Lutzomyia. Wyróżnia się trzy postaciechoroby: skórną, śluzówkowo-skórną i trzewną. Cel pracy: Zwrócenie uwagi na narastający problem zawlekaniachorób z krajów tropikalnych i trudności w rozpoznaniu skórnej lei szmaniozy. Opis przypadku: Pacjent, lat 29, został przyjęty do Kliniki z powoduowrzodzenia II palca stopy lewej po powrocie z 16-dniowego pobytuw Maroku. Początkowo był diagnozowany w miejscu zamieszkania,gdzie wykluczono leiszmaniozę, opierając się na negatywnych wynikachbadań zeskrobin skórnych i testów serologicznych. Po przyjęciudo Kliniki pobrano wycinek ze zmiany skórnej palca do badania histopatologicznego,w którym stwierdzono zmiany sugerujące tę chorobę.Do leczenia włączono ketokonazol na 4 tygodnie, uzyskując niewielkąpoprawę. Po tej terapii obserwowano stałą, choć wolną, poprawę miejscowąi po kolejnych 3 miesiącach doszło do całkowitego wygojeniazmian skórnych. Wnioski: W procesie diagnostycznym skórnej leiszmaniozy należyuwzględnić ocenę histopatologiczną wycinka ze zmiany. W związkuz coraz częstszymi wyjazdami do stref występowania leiszmaniozynależy oczekiwać zwiększenia liczby przypadków tej choroby w Polsce.

  12. Glacial geomorphology of the Torres del Paine region (southern Patagonia): Implications for glaciation, deglaciation and paleolake history

    Science.gov (United States)

    García, Juan-Luis; Hall, Brenda L.; Kaplan, Michael R.; Vega, Rodrigo M.; Strelin, Jorge A.

    2014-01-01

    The processes affecting paleoclimate variability and Pleistocene glacial landscape development in the southern mid-latitudes remain poorly understood, in part because of the scarcity of comprehensive, well-studied records. Glacial landforms are invaluable for reconstructing past ice-sheet, climate, and associated environmental changes along the southern Andes, but there are significant spatial and temporal gaps in existing data. In this paper, we present new geomorphic and sedimentologic analyses, including surficial maps, for the Torres del Paine region (51°S, 73°W), southern South America. Our findings provide a new framework for understanding changes in the regional glacier history and Pleistocene landscape development. Glacial extent during the local last glacial maximum (LGM) remains unknown but new chronological data supported by geomorphic evidence afford evidence for a larger ice sheet at Torres del Paine than previously assumed. Deglaciation from the local LGM was underway by 17,400 ± 200 (1σ) cal. yr. BP. As opposed to previous suggestions, we have found that most of the moraines fringing the lakes in the Torres del Paine national park were deposited during a late-glacial expansion that occurred between 14,100 and 12,500 cal. yr. BP. Late-glacial advances also have been documented recently for the Última Esperanza and Lago Argentino basins to the south and north of Torres del Paine, respectively, suggesting an overall regional ice response to a climate signal. The Tehuelche paleolake accompanied each of the ice-sheet fluctuations in Torres del Paine. New data document at least three main phases of this paleolake, which drained eastward to the Atlantic Ocean, while the Andes gaps were blocked with ice. During the late phase of glacial lake formation, when water levels reached 125-155 m a.s.l., the lake likely merged with paleolake Consuelo in the Última Esperanza area at the end of the last glaciation. Lake Tehuelche in Torres del Paine had drained

  13. THE PROBLEM OF COMMON SLAVIC OPPOSITION X ~ SK ~ ŠČ

    Directory of Open Access Journals (Sweden)

    A. K. Shaposhnikov

    2015-01-01

    Full Text Available This article deals with Common Slavic rows of lexical oppositions with x ~ sk ~ šč, going back to primordial Indo-European sound clusters *sk or *ks, and presents an attempt at establishing laws and rules of their occurrence. There is one more Indo-European language, namely Albanian, in which there was a similar regular transition of etymological *sk, *ks into *h in initial, intervocal and final positions in the words. All examples of similar opposition known nowadays from ESSJA and other accessible publications are presented for further consideration of the similar phenomena. About 160 examples of different degrees of reliability  are submitted. The author deduces some rules on the basis of comparisons: 1 etymological initial *skregularly passed into *xbefore back vowels. Reconstruction of forms with s mobile is an anachronism. 2 etymological initial *skhas regularly changed into *ščbefore front vowels. 3 Etymological initial *skwas kept only after a prefix *ob-. 4 It is possible to explain preservation of etymological initial *skin some cases by a loss of a prefixal morpheme: *skъrbь  from *obskъrbь.  The mechanism of mutation seems to be as follows: skhas changed into ks(this stage of development: Sanskrit kṣoda  and Greek , and then by the notorious rule of RUKI kshas changed into khand later into x-. The etymological confluence skbefore a back vowel is kept only after prefix *ob(*obskalъ, *obskǫděti, *obskomina. Regular opposition initial x-: initial  šč-, can never  be sk-: šč-, therefore ščel’, ščyolka  correlate with oskal but skala,  skol  < *sъkala, *sъkolъ. In this case we have an etymological opposition *xo-: *sъ-ko-.

  14. Disease specific enrichment of circulating let-7 family microRNA in MuSK+ myasthenia gravis.

    Science.gov (United States)

    Punga, Tanel; Bartoccioni, Emanuela; Lewandowska, Marta; Damato, Valentina; Evoli, Amelia; Punga, Anna Rostedt

    2016-03-15

    Myasthenia gravis (MG) patients with antibodies against the muscle specific tyrosine kinase (MuSK+) have predominantly involvement of cranio-bulbar muscles and do not display thymus pathology, as do acetylcholine receptor antibody seropositive (AChR+) MG patients. In search of novel biomarkers for MuSK+ MG, we evaluated circulating serum microRNAs. Four analyzed microRNAs were specifically elevated in MuSK+ MG patient serum samples: let-7a-5p, let-7f-5p, miR-151a-3p and miR-423-5p. The circulating microRNA profile in MuSK+ MG differs from the profile previously observed in the serum of AChR+ MG, thus indicating the etiological difference between these two entities. We propose that the identified microRNAs could serve as potential serum biomarkers for MuSK+ MG.

  15. Rem Koolhaas y la nueva Babel. De la torre metropolitana al monumento al vacío

    Directory of Open Access Journals (Sweden)

    José Antonio Tallón

    2015-05-01

    Full Text Available Un primer acercamiento a las reflexiones de Rem Koolhaas en torno a la tipología de torre introduce al rascacielos neoyorquino como la alegoría del “automonumen­to”: una construcción en esencia destinada a reafirmar su sola presencia y que se distingue del resto por medio de su estatura, que la monumentaliza. La torre de Babel, símbolo inquebrantable de la leyenda de la construcción en altura, escenifi­ca una historia de construcción y destrucción que está vinculada ineludiblemente al pensamiento crítico de Rem Koolhaas en torno a la torre como tipología desa­creditada. Un recorrido por las distintas “Babel” que Rem Koolhaas cataloga en el glosario de términos incluido en el texto SMLXL construye un discurso en torno a la destrucción de la torre bíblica y la construcción de la nueva Babel koolhaasiana que inicia su recorrido con el rascacielos para acabar reclamando un nuevo estado de monumentalidad: la ausencia en su estado más puro representado por el muro, el máximo ejemplo de ausencia como la forma más elevada de presencia monu­mental. Una mirada crítica que comienza con la torre metropolitana como la nueva Babel para finalizar con el muro como el monumento al vacío

  16. Measuring emotional and social wellbeing in Aboriginal and Torres Strait Islander populations: an analysis of a Negative Life Events Scale

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    Gunthorpe Wendy

    2007-11-01

    Full Text Available Abstract Aboriginal and Torres Strait Islander Australians experience widespread socioeconomic disadvantage and health inequality. In an attempt to make Indigenous health research more culturally-appropriate, Aboriginal and Torres Strait Islander Australians have called for more attention to the concept of emotional and social wellbeing (ESWB. Although it has been widely recognised that ESWB is of crucial importance to the health of Aboriginal and Torres Strait Islander peoples, there is little consensus on how to measure in Indigenous populations, hampering efforts to better understand and improve the psychosocial determinants of health. This paper explores the policy and political context to this situation, and suggests ways to move forward. The second part of the paper explores how scales can be evaluated in a health research setting, including assessments of endorsement, discrimination, internal and external reliability. We then evaluate the use of a measure of stressful life events, the Negative Life Events Scale (NLES, in two samples of Aboriginal people living in remote communities in the Northern Territory of Australia. We argue that the Negative Life Events Scale is a promising assessment of psychosocial wellbeing in Aboriginal and Torres Strait Islander populations. Evaluation of the scale and its performance in other samples of Aboriginal and Torres Strait Islander populations is imperative if we hope to develop better, rather than more, scales for measuring ESWB among Indigenous Australians. Only then will it be possible to establish standardized methods of measuring ESWB and develop a body of comparable literature that can guide both a better understanding of ESWB, and evaluation of interventions designed to improve the psychosocial health of Indigenous populations and decrease health inequalities.

  17. Aboriginal and Torres Strait Islander Worldviews and Cultural Safety Transforming Sexual Assault Service Provision for Children and Young People

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    Leticia Funston

    2013-08-01

    Full Text Available Child Sexual Assault (CSA in Aboriginal and Torres Strait Islander communities is a complex issue that cannot be understood in isolation from the ongoing impacts of colonial invasion, genocide, assimilation, institutionalised racism and severe socio-economic deprivation. Service responses to CSA are often experienced as racist, culturally, financially and/or geographically inaccessible. A two-day forum, National Yarn Up: Sharing the Wisdoms and Challenges of Young People and Sexual Abuse, was convened by sexual assault services to identify the main practice and policy concerns regarding working with Aboriginal and Torres Strait Islander children and young people (C&YP, families and communities in the context of CSA. The forum also aimed to explore how services can become more accountable and better engaged with the communities they are designed to support. The forum was attended by eighty invited Aboriginal and Torres Strait Islander and non-Aboriginal youth sexual assault managers and workers representing both “victim” and “those who sexually harm others” services. In keeping with Aboriginal Community-Based Research methods forum participants largely directed discussions and contributed to the analysis of key themes and recommendations reported in this article. The need for sexual assault services to prioritise cultural safety by meaningfully integrating Aboriginal and Torres Strait Islander Worldviews emerged as a key recommendation. It was also identified that collaboration between “victims” and “those who sexually harm” services are essential given Aboriginal and Torres Strait Islander C&YP who sexually harm others may have also been victims of sexual assault or physical violence and intergenerational trauma. By working with the whole family and community, a collaborative approach is more likely than the current service model to develop cultural safety and thus increase the accessibility of sexual assault services.

  18. Numerical simulations of highly buoyant flows in the Castel Giorgio - Torre Alfina deep geothermal reservoir

    Science.gov (United States)

    Volpi, Giorgio; Crosta, Giovanni B.; Colucci, Francesca; Fischer, Thomas; Magri, Fabien

    2017-04-01

    Geothermal heat is a viable source of energy and its environmental impact in terms of CO2 emissions is significantly lower than conventional fossil fuels. However, nowadays its utilization is inconsistent with the enormous amount of energy available underneath the surface of the earth. This is mainly due to the uncertainties associated with it, as for example the lack of appropriate computational tools, necessary to perform effective analyses. The aim of the present study is to build an accurate 3D numerical model, to simulate the exploitation process of the deep geothermal reservoir of Castel Giorgio - Torre Alfina (central Italy), and to compare results and performances of parallel simulations performed with TOUGH2 (Pruess et al. 1999), FEFLOW (Diersch 2014) and the open source software OpenGeoSys (Kolditz et al. 2012). Detailed geological, structural and hydrogeological data, available for the selected area since early 70s, show that Castel Giorgio - Torre Alfina is a potential geothermal reservoir with high thermal characteristics (120 ° C - 150 ° C) and fluids such as pressurized water and gas, mainly CO2, hosted in a carbonate formation. Our two steps simulations firstly recreate the undisturbed natural state of the considered system and then perform the predictive analysis of the industrial exploitation process. The three adopted software showed a strong numerical simulations accuracy, which has been verified by comparing the simulated and measured temperature and pressure values of the geothermal wells in the area. The results of our simulations have demonstrated the sustainability of the investigated geothermal field for the development of a 5 MW pilot plant with total fluids reinjection in the same original formation. From the thermal point of view, a very efficient buoyant circulation inside the geothermal system has been observed, thus allowing the reservoir to support the hypothesis of a 50 years production time with a flow rate of 1050 t

  19. α-actinin2增加SK2通道在HEK293膜蛋白的表达%α-Actinin 2 increases membrane protein expression of SK2 channel in HEK293 cell

    Institute of Scientific and Technical Information of China (English)

    李涛; 陈桂兰; 黄文俊; 毛亮; 李畅; 杨艳; 曾晓荣

    2014-01-01

    目的:证实α-Actinin 2与小电导钙激活钾通道(Small Conductance Ca2+-activated K+Channels, SK)的共同表达增加SK2在HEK293细胞膜上的表达。方法:HEK293细胞用RPMI 1640培养基培养。将HEK293细胞分为两组,对照组细胞转染pIRES-SK2质粒,实验组细胞共转染pIRES-SK2和pcDNA3-α-actinin2质粒。免疫荧光共聚焦显微镜和蛋白印迹技术检测SK2通道蛋白在HEK293细胞膜上的表达。结果:免疫荧光共聚焦显微镜检测到转染pIRES-SK2质粒的HEK293细胞膜上有SK2通道蛋白的表达。蛋白印迹技术显示实验组蛋白条带亮度明显高于对照组。结论:α-Actinin 2能增加SK2在HEK293细胞膜上的表达。%Objective:To confirm whether the protein expression of SK2 channel in HEK293 cell membrane is increased by the co-expression of α-Actinin2. Methods:HEK293 cells were cultured and randomly divided into control group, transfected with plasmid pIRES-SK2,and experimental group,transfected with plasmid pIRES-SK2 plus pcDNA3-α-Actinin2. Immunofluorescence confocal microscopy and western-blotting were used to test the membrane protein expression of SK2 channel in HEK293 cell. Results: SK2 channel protein was detected in the membrane of HEK293 by immunofluorescence confocal microscopy ,and he protein expression level in the experimental group was much higher than that in the control group. Conclusion:α-Actinin2 can increase SK2 channel membrane protein expression in HEK293 cell.

  20. Torre «Reunión», en Dallas Texas - EE. UU.

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    Becket, Welton

    1980-12-01

    Full Text Available The Reunion Tower, 50 stories and 170 m high, along with the adjacent 1,000 room hotel, constitutes the first phase of an urbanistic rehabilitation complex for a plot of 20 hectares, located in the South-West sector of the city's shopping centre. Due to its singular characteristics, the Tower has in fact already become a clear identifying mark in the city of Dallas. Right at the top, it ends in a geodesic dome, in three levels, that amount to over 2,300 m2 of the total area, and where there is a restaurant on rotating platform, a cocktail room of the same characteristics, an observation terrace and a radio station. The project has been given the award of the Reinforced Cement Institute, and that of the American Institute of Architects (AIA corresponding to Los Angeles.

    La Torre «Reunión», de 50 plantas y 170 m de altura constituye, junto con el hotel de 1.000 habitaciones adyacente, la primera fase de un complejo de rehabilitación urbanística para una parcela de 20 hectáreas situada en el sector suroeste del centro comercial de la ciudad. De hecho, por sus singulares características, la Torre se ha convertido ya en una clara señal de identidad de la ciudad de Dallas. En su extremo superior está rematada por una cúpula geodésica, con tres niveles que suman más de 2.300 m2 de superficie total, y en los que están distribuidos un restaurante sobre plataforma giratoria, una sala de cocktails de iguales características, una terraza de observación y una emisora de radio. El proyecto ha merecido el premio concedido por el Instituto del Cemento Armado, y el del Instituto Americano de Arquitectos (AIA, correspondiente a Los Angeles.

  1. Bioreactor Cultivation of Zeltnera beyrichii (Torr. & A. Gray Mans.: A Novel Source of Biologically Active Compounds

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    Miloš Radović

    2013-08-01

    Full Text Available With regard to world’s increasing demand for biologically active compounds, a novel source of xanthones and secoiridoid glycosides has been studied . Zeltnera beyrichii (Torr. & A. Gray Mans., an insufficiently acknowledged North American medicinal plant species, may be considered a pharmacological substitute for commercial C. erythraea Rafn, since it accumulates in aerial parts nearly the same amount of secoiridoid glycosides: swertiamarin, gentiopicrin, and sweroside (13.76, 7.56, and 0.17 mmol per 100 g dry weight, respectively in plants grown under greenhouse condition, and a considerable amount of xanthones: decussatin and eustomin. Additionally, Z. beyrichii produced as much biomass during cultivation in RITA ® temporary immersion bioreactors as greenhouse-grown plants, in a third of the time. Plants grown in bioreactors contained moderate levels of total phenolics and total flavonoids, and possessed modest antioxidant activity and antimicrobial potential against eight bacterial and eight fungal species. Therefore, this species may be highly recommended for cultivation either in natural environment, or in bioreactors under in vitro conditions, for producing compounds of interest of modern pharmacology and food industry.

  2. Torres de vidrio del centro de arte "Reina Sofía" de Madrid

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    Íñiguez de Onzoño, José Luis

    1992-02-01

    Full Text Available The elevator towers in the Art Center "Reina Sofía" stand out in their urban setting due to their transparent partition against the opaque façade of the old "Hospital Provincial de Atocha". The material and functional characteristics of their partition are the topic of this article: - The bracing and fixation system against gravitation and thermic forces through vertical steel ties, special joints and springs in the bases. - The bracing and fixation system against horizontal actions on the corners of each piece. - The function of structural silicone as the only material between panels helping in the redistribution of stress.

    Las torres de ascensores del Centro de Arte "Reina Sofía", destacan en su entorno urbano debido a su importante cerramiento transparente, frente a la opaca fachada del antiguo Hospital Provincial de Atocha. Las características materiales y de funcionamiento de su cerramiento es lo que aquí se expone: — El sistema de anclaje y fijación ante las acciones gravitatorias y térmicas a través de tirantes verticales de acero, rótulas especiales, y muelles en sus bases. — El sistema de anclaje y fijación ante acciones horizontales en las esquinas de cada pieza. — La función de la silicona estructural, como único material de enlace entre paneles para colaborar en la redistribución de esfuerzos.

  3. Removal of lead ions from aqueous solution by the dried aquatic plant, Lemna perpusilla Torr.

    Science.gov (United States)

    Tang, Yankui; Chen, Ling; Wei, Xingren; Yao, Qiuyan; Li, Ting

    2013-01-15

    The aquatic plant, Lemna perpusilla Torr. strain, was dried, pulverized and used for Pb(II) removal from aqueous solution. Batch experiments were conducted to investigate the effects of pH, contact time, initial metal concentration and temperature on Pb(II) adsorption. A dose of 4 g L(-1) of dried L. perpusilla in a solution with an initial pH of 4.6, an initial Pb(II) concentration of 50 mg L(-1) and a contact time of 210 min resulted in the maximum Pb(II) removal efficiency (above 95%). The equilibrium adsorption capacities increased with increasing initial Pb(II) concentration. The adsorption isotherm was better described by a Langmuir model rather than a Freundlich model. Further, the adsorption kinetics followed a pseudo-second-order model. An FTIR examination revealed changes between the natural and Pb(II)-loaded plant material. Scanning electron micrograph (SEM) also revealed changes in the surface morphology of the biomass as a result of lead adsorption. Based on these results, it can be concluded that the dried L. perpusilla is effective in removing lead from aqueous solution and merits consideration for scaled-up trials.

  4. Eating disorder features in indigenous Aboriginal and Torres Strait Islander Australian Peoples

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    Hay Phillipa J

    2012-03-01

    Full Text Available Abstract Background Obesity and related cardiovascular and metabolic conditions are well recognized problems for Australian Aboriginal and Torres Strait Islander peoples. However, there is a dearth of research on relevant eating disorders (EDs such as binge eating disorder in these groups. Methods Data were obtained from interviews of 3047 (in 2005 and 3034 (in 2008 adults who were participants in a randomly selected South Australian household survey of individuals' age > 15 years. The interviewed comprised a general health survey in which ED questions were embedded. Data were weighted according to national census results and comprised key features of ED symptoms. Results In 2005 there were 94 (85 weighted First Australian respondents, and in 2008 65 (70 weighted. Controlling for secular differences, in 2005 rates of objective binge eating and levels of weight and shape influence on self-evaluation were significantly higher in indigenous compared to non-indigenous participants, but no significant differences were found in ED features in 2008. Conclusions Whilst results on small numbers must be interpreted with caution, the main finding was consistent over the two samples. For First Australians ED symptoms are at least as frequent as for non-indigenous Australians.

  5. Hipogeos funerarios del Bronce Pleno en Torre Velha 3 (Serpa, Portugal.

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    Catarina ALVES

    2011-04-01

    Full Text Available Recientes intervenciones arqueológicas realizadas en el Bajo Alentejo (Portugal, en el ámbito del Proyecto de Alqueva (EDIA, han permitido la identificación de importantes vestigios del Bronce del Sudoeste. En este artículo se presentan los primeros datos de uno de los mayores conjuntos de hipogeos funerarios de esa época, excavado en el yacimiento de Torre Velha 3 (Serpa, que muestra claras semejanzas con el mundo argárico, sobre todo en los rituales, en la arquitectura y en el ajuar ofrendado. Así, los hipogeos están formados por un atrio, que puede tener diversas formas y que da acceso a una se encuentran generalmente en la cámara, y se acompañan de artefactos cerámicos y metálicos y de ofrendas cárnicas, lo que constituye un indicio de la realización de rituales de comensalidad con ocasión de los en- tierros. La datación por radiocarbono de muestra óseas pertenecientes a las ofrendas de carne permitió asignar a estos hipogeos una cronología dentro del segundo cuarto/inicios del tercero del II Milenio a.C.

  6. Suicides in Aboriginal and Torres Strait Islander children: analysis of Queensland Suicide Register.

    Science.gov (United States)

    Soole, Rebecca; Kõlves, Kairi; De Leo, Diego

    2014-12-01

    Suicide rates among Indigenous Australian children are higher than for other Australian children. The current study aimed to identify factors associated with Indigenous child suicide when compared to other Australian children. Using the Queensland Suicide Register, suicides in Indigenous children (10-14 years) and other Australian children in the same age band were compared. Between 2000 and 2010, 45 child suicides were recorded: 21 of Indigenous children and 24 of other Australian children. This corresponded to a suicide rate of 10.15 suicides per 100,000 for Indigenous children - 12.63 times higher than the suicide rate for other Australian children (0.80 per 100,000). Hanging was the predominant method used by all children. Indigenous children were significantly more likely to suicide outside the home, to be living outside the parental home at time of death, and be living in remote or very remote areas. Indigenous children were found to consume alcohol more frequently before suicide, compared to other Australian children. Current and past treatments of psychiatric disorders were significantly less common among Indigenous children compared to other Australian children. Western conceptualisation of mental illness may not adequately embody Indigenous people's holistic perspective regarding mental health. Further development of culturally appropriate suicide prevention activities for Aboriginal and Torres Strait Islander children is required. © 2014 Public Health Association of Australia.

  7. Entrevista de Domenico Bilotti a Massimo La Torre sobre Goliarda Sapienza

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    Domenico Bilotti

    2014-07-01

    Full Text Available No se ha dicho ni escrito mucho (y quizás menos se ha leído sobre Goliarda Sapienza (1924-1996, actriz de cine y de teatro, escritora y poetisa que ha atravesado, con una gracia inaudita, los restos de una Italia que había soñado con el boom económico y, veinte años después, las ruinas de una Italia que había soñado con la revolución. Merecidamente, la editorial de Turín Einaudi está publicando de nuevo su catálogo de obras completas, dando a la autora siciliana finalmente el escaparate que habría merecido ya en vida. Conversamos sobre ello con Massimo La Torre, profesor de Filosofía del Derecho en la Universidad de Catanzaro, columnista de Critica Liberale, y, por pasión, conocedor de las artes cinematográficas y de la literatura siciliana.

  8. Magmatic evolution of the Early Pliocene Etrüsk stratovolcano, Eastern Anatolian Collision Zone, Turkey

    Science.gov (United States)

    Oyan, Vural; Keskin, Mehmet; Lebedev, Vladimir A.; Chugaev, Andrey V.; Sharkov, Evgenii V.

    2016-07-01

    The Pliocene Etrüsk stratovolcano, located in the northeast of Lake Van (Eastern Anatolia; Turkey), is one of the important volcanic centres in the Eastern Anatolian collision zone. Mt. Etrüsk overlies a widespread volcanic plateau, consisting of basaltic and hawaiitic lavas formed by fissure eruptions between 4.9-4.5 Ma. These basic lavas contain a phenocryst phase consisting of olivine, plagioclase and clinopyroxene. Trace element ratio diagrams imply that these basic magmas were generated from a mantle that contained a clear subduction component that is related to the subducted sediments rather than fluids or altered oceanic crust. Results of the melting models on the basaltic plateau lavas indicate that there was a marked variation both in the mantle source mineralogy (i.e. the ratio of garnet peridotite to spinel peridotite in the source varies between 60/40% and 40/60%) and the degree of melting (i.e. F between 0.8-4%). This can be explained by a model in which magmas were generated by partial melting of both metasomatised lithospheric and deeper asthenospheric mantle sources in an extensional setting in response to the partial delamination of the lithospheric mantle of Eastern Anatolia and then mixed with each other during Pliocene times. Central eruptions that formed the Etrüsk volcano lasted ~ 600 kyr between 4.3-3.7 Ma during Zanclean times. The estimated depth of the Etrüsk magma chamber is ~ 9-12 km. The volcano erupted lavas with a rather narrow compositional range from latite to rhyolite, which are either transitional or mildly alkaline in character. The Etrüsk lavas contain plagioclase, clino- and orthopyroxene, biotite, K-feldspar and rarely, minor amounts of olivine and amphibole in the phenocryst phase. A composite chemo-stratigraphic section of the volcano and petrological models indicate that the evolved lavas of the Etrüsk volcano differentiated from a parental magma composition, which is similar to that of the most primitive plateau

  9. Enhancing national data to align with policy objectives: Aboriginal and Torres Strait Islander smoking prevalence at finer geographic levels.

    Science.gov (United States)

    Wright, Alyson; Lovett, Ray; Roe, Yvette; Richardson, Alice

    2017-06-05

    Objectives The aim of the study was to assess the utility of national Aboriginal survey data in a regional geospatial analysis of daily smoking prevalence for Aboriginal and Torres Strait Islander Australians and discuss the appropriateness of this analysis for policy and program impact assessment.Methods Data from the last two Australian Bureau of Statistics (ABS) national surveys of Aboriginal and Torres Strait Islander people, the National Aboriginal and Torres Strait Islander Social Survey 2014-15 (n=7022 adults) and the National Aboriginal and Torres Strait Islander Health Survey 2012-13 (n=10896 adults), were used to map the prevalence of smoking by Indigenous regions.Results Daily smoking prevalence in 2014-15 at Indigenous regions ranges from 27.1% (95%CI 18.9-35.3) in the Toowoomba region in Queensland to 68.0% (95%CI 58.1-77.9) in the Katherine region in the Northern Territory. The confidence intervals are wide and there is no significant difference in daily smoking prevalence between the two time periods for any region.Conclusion There are significant limitations with analysing national survey data at finer geographical scales. Given the national program for Indigenous tobacco control is a regional model, evaluation requires finer geographical analysis of smoking prevalence to inform public health progress, policy and program effects. Options to improve the data currently collected include increasing national survey sample sizes, implementing a smoking status question in census surveys, investing in current cohort studies focused on this population or implementing localised surveys.What is known about the topic? The last geospatial analysis of Aboriginal and Torres Strait Islander smoking prevalence was undertaken in 1997. Current national survey data have not been analysed geospatially.What does this paper add? This paper provides new insights into the use of national survey data for understanding regional patterns and prevalence levels of smoking in

  10. Strategic approaches to enhanced health service delivery for Aboriginal and Torres Strait Islander people with chronic illness: a qualitative study

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    Aspin Clive

    2012-06-01

    Full Text Available Abstract Background Aboriginal and Torres Strait Islander people with chronic illness confront multiple challenges that contribute to their poor health outcomes, and to the health disparities that exist in Australian society. This study aimed to identify barriers and facilitators to care and support for Aboriginal and Torres Strait Islander people with chronic illness. Methods Face-to-face in-depth interviews were conducted with Aboriginal and Torres Strait Islander people with diabetes, chronic heart failure or chronic obstructive pulmonary disease (n-16 and family carers (n = 3. Interviews were transcribed verbatim and the transcripts were analysed using content analysis. Recurrent themes were identified and these were used to inform the key findings of the study. Results Participants reported both negative and positive influences that affected their health and well-being. Among the negative influences, they identified poor access to culturally appropriate health services, dislocation from cultural support systems, exposure to racism, poor communication with health care professionals and economic hardship. As a counter to these, participants pointed to cultural and traditional knowledge as well as insights from their own experiences. Participants said that while they often felt overwhelmed and confused by the burden of chronic illness, they drew strength from being part of an Aboriginal community, having regular and ongoing access to primary health care, and being well-connected to a supportive family network. Within this context, elders played an important role in increasing people’s awareness of the impact of chronic illness on people and communities. Conclusions Our study indicated that non-Indigenous health services struggled to meet the needs of Aboriginal and Torres Strait Islander people with chronic illness. To address their complex needs, health services could gain considerably by recognising that Aboriginal and Torres Strait

  11. Isolation, characterization and anti-cancer activity of SK84, a novel glycine-rich antimicrobial peptide from Drosophila virilis.

    Science.gov (United States)

    Lu, Jie; Chen, Zheng-wang

    2010-01-01

    We report herein the isolation and characterization of a novel glycine-rich antimicrobial peptide purified from the larvae of Drosophila virilis. A range of chromatographic methods was used for isolation and its antibacterial activity against Bacillus subtilis was employed to screen for the most active fractions. The peptide, termed SK84 due to its N-terminal serine, C-terminal lysine and a total of 84 residues, was completed sequenced using RT-PCR cDNA cloning. SK84 contains a high level of glycine (15.5%) and a hexaglycine cluster motif in the N-terminal part. SK84 displayed antibacterial activity against the tested Gram-positive bacteria (B. subtilis, Bacillus thuringiensis and Staphylococcus aureus), but had no effect on Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli) and fungi (Saccharomyces cerevisiae, Candida albicans). SK84 had specific inhibitory effects on the proliferation of several cancer cell lines (Human leukemia THP-1, liver cancer HepG2, and breast cancer MCF-7 cells), but no hemolytic activity. The results from scanning electron microscopy observations revealed that SK84 killed THP-1 cells by destroying the cell membranes. Alignment results show that SK84 is a mature protein processed from the pseudoprotein GJ19999 from D. virilis, and is very similar to several pseudoproteins from different Drosophila species. Our results show that SK84 represents a novel glycine-rich peptide family in Drosophila species with antimicrobial and anti-cancer cell activities.

  12. Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings.

    Science.gov (United States)

    Samuilov, V D; Kiselevsky, D B

    2015-04-01

    Plastoquinone bound with decyltriphenylphosphonium cation (SkQ1) penetrating through the membrane in nanomolar concentrations inhibited H2O2 generation in cells of epidermis of pea seedling leaves that was detected by the fluorescence of 2',7'-dichlorofluorescein. Photosynthetic electron transfer in chloroplasts isolated from pea leaves is suppressed by SkQ1 at micromolar concentrations: the electron transfer in chloroplasts under the action of photosystem II or I (with silicomolybdate or methyl viologen as electron acceptors, respectively) is more sensitive to SkQ1 than under the action of photosystem II + I (with ferricyanide or p-benzoquinone as electron acceptors). SkQ1 reduced by borohydride is oxidized by ferricyanide, p-benzoquinone, and, to a lesser extent, by silicomolybdate, but not by methyl viologen. SkQ1 is not effective as an electron acceptor supporting O2 evolution from water in illuminated chloroplasts. The data on suppression of photosynthetic O2 evolution or consumption show that SkQ1, similarly to phenazine methosulfate, causes conversion of the chloroplast redox-chain from non-cyclic electron transfer mode to the cyclic mode without O2 evolution. Oxidation of NADH or succinate in mitochondria isolated from pea roots is stimulated by SkQ1.

  13. Detection of Large Color Variation of Potentially Hazardous Asteroid (297274) 1996 SK

    CERN Document Server

    Lin, Chien-Hsien; Lin, Zhong-Yi; Yoshida, Fumi; Cheng, Yu-Chi

    2013-01-01

    Low-inclination Near-Earth Asteroid (297274) 1996 SK, which is also classified as a Potentially Hazardous Asteroid, has a highly eccentric orbit. It was studied by multi-wavelength photometry within the framework of an NEA color survey at the Lulin Observatory. We report here the finding of large color variation across the surface of (297274) 1996 SK within one asteroidal rotation period of $4.656\\pm0.122$ hours and classify it as a S-type asteroid according to its average colors of $B-V=0.767\\pm0.033$, $V-R=0.482\\pm0.021$, $V-I=0.801\\pm0.025$ and the corresponding relative reflectance spectrum. It might be indicative of differential space weathering effect or compositional inhomogeneity of the surface materials.

  14. Muscle-Specific Receptor Tyrosine Kinase (MuSK) Myasthenia Gravis.

    Science.gov (United States)

    Hurst, Rebecca L; Gooch, Clifton L

    2016-07-01

    Autoimmune myasthenia gravis (MG) is the prototypic, antibody-mediated neuromuscular disease and is characterized by a decrease in the number of functional acetylcholine receptors (AChR) within the muscle end plate zone of the neuromuscular junction (NMJ). Although the pathophysiology of AChR-mediated myasthenia gravis has been extensively studied over the last 40 years since its original description by Patrick and Lindstrom (Science 180:871-872, 1973), less is known about the much more recently described muscle-specific kinase (MuSK) antibody-mediated MG. MuSK-MG has features clinically distinct from Ach-R MG, as well as a different pattern of response to treatment and a unique immunopathogenesis.

  15. Astronomical journey to the Skåne district in Sweden

    Science.gov (United States)

    Nakamura, Tsuko

    2006-09-01

    In September of 2005, we had a chance to travel to the Skåe district of Sweden. This short paper briefly reports what we saw there, along with historical episodes relating to Lund Observatory, the medieval astronomical clock preserved at the Lund Cathedral, and the Hven island where Tycho Brahe constructed in the late 16th century the first modern astronomical observatory called Uraniborg.

  16. Skærmstilling og underplantning af rødgran i Gludsted Plantage

    DEFF Research Database (Denmark)

    Brunner, Andreas; Sørensen, Ib Holmgård; Skovsgaard, Jens Peter

    Konvertering af rødgran til mere stabile blandingsbevoksninger er blevet et vigtigt mål i mange skove og betragtes som regel som et led i indførelsen af naturnær skovdrift. Rapporten beskriver et forsøg med skærmstilling og underplantning af rødgran in Gludsted Plantage og første resultater fra f...

  17. EarthLink和SK Telecom的合资公司正式成立

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    美国网络服务提供商EarthLink和韩国最大的通信公司韩国电信(SK Telecom)合资成立的移动虚拟网络运营商(Mobile Virtual Network Operator,MVNO)已组建完成,这家名称为SK—EarthLink的合资企业即将进人美国的无线服务市场。

  18. Observation of the decay B¯s0→ψ(2SK+π−

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    R. Aaij

    2015-07-01

    Full Text Available The decay B¯s0→ψ(2SK+π− is observed using a data set corresponding to an integrated luminosity of 3.0 fb−1 collected by the LHCb experiment in pp collisions at centre-of-mass energies of 7 and 8 TeV. The branching fraction relative to the B0→ψ(2SK+π− decay mode is measured to beB(B¯s0→ψ(2SK+π−B(B0→ψ(2SK+π−=5.38±0.36(stat±0.22(syst±0.31(fs/fd%, where fs/fd indicates the uncertainty due to the ratio of probabilities for a b quark to hadronise into a Bs0 or B0 meson. Using an amplitude analysis, the fraction of decays proceeding via an intermediate K⁎(8920 meson is measured to be 0.645±0.049(stat±0.049(syst and its longitudinal polarisation fraction is 0.524±0.056(stat±0.029(syst. The relative branching fraction for this component is determined to be B(B¯s0→ψ(2SK⁎(8920B(B0→ψ(2SK⁎(8920=5.58±0.57(stat±0.40(syst±0.32(fs/fd%. In addition, the mass splitting between the Bs0 and B0 mesons is measured as M(Bs0−M(B0=87.45±0.44(stat±0.09(syst MeV/c2.

  19. El arquitecto madrileño Pedro de la Torre en Toledo y un retablo inédito localizado

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    Antonio José Díaz Fernández

    2014-02-01

    Full Text Available Entre las realizaciones del arquitecto barroco Pedro de la Torre para la ciudad de Toledo es posible identificar un retablo hasta ahora inédito, datado en 1653 y en el que colabora el ensamblador toledano Juan García de San Pedro, localizado hoy en la parroquia de Nambroca (Toledo, en el que se aprecian las innovaciones estructurales y ornamentales propias del lenguaje desarrollado por el artista.Among the works of the baroque architect Pedro de la Torre for the city of Toledo is possible to identify an up to now unpublished altarpiece in which collaborates the toledan assembler Juan García de San Pedro. Dated in 1653 and at present located in the parish of Nambroca (Toledo, in which we can appreciate the typical structural and ornamental innovations of the language developed by the artist.

  20. La meridiana di Egnazio Danti nella Torre dei Venti in Vaticano: un'icona della riforma Gregoriana del calendario

    Science.gov (United States)

    Sigismondi, Costantino

    2014-05-01

    La Torre dei Venti domina l’angolo Sud Ovest del cortile della Pigna (nell'area dei Musei Vaticani), ed è inclusa negli ambienti dell'Archivio Segreto Vaticano. Non è aperta al pubblico, ma è universalmente nota per la fama che da oltre quattrocento anni la circonda, legata alle vicende della riforma Gregoriana del calendario. La meridiana tracciata da padre Egnazio Danti (1536-1586) nella torre dei Venti, fu visitata anche da Gregorio XIII, probabilmente il 21 marzo 1581 come suppone il padre Stein, per convincersi dell'anticipo ormai arrivato a dieci giorni dell'equinozio di primavera sulla data che il concilio di Nicea aveva fissato al 21 marzo per il computo pasquale. La ricognizione astrometrica del febbraio-marzo 2009 fatta dall'autore viene qui presentata.

  1. MaSK: A visualization tool for teaching and research in computational chemistry

    Science.gov (United States)

    Podolyan, Yevgeniy; Leszczynski, Jerzy

    The number of researchers using computational chemistry tools is growing every year. There are multiple programs used for calculation of various molecular and electronic properties such as optimized geometry, energy, vibrational spectra, and so forth. Another set of programs is used for the visualization of these properties. However, such programs are either too complex for a beginner or too simple for an intermediate user for everyday use. Molecular Modeling and Simulation Kit (MaSK) is designed to fill this gap by presenting an easy-to-use intuitive interface to quantum chemical programs such as GAMESS and Gaussian with an array of advanced tools. The program can be used as a postprocessor to visualize calculated properties or as a preprocessor to prepare the input files for quantum chemical programs. In addition, some properties such as the surfaces of molecular orbitals, electron and spin densities, and molecular electrostatic potentials are actually calculated by MaSK. If MaSK is combined with PC GAMESS, the preparation of the input, running of the calculations, and the display of the results can all be done without leaving the program's interface.

  2. Structural basis of agrin-LRP4-MuSK signaling

    Energy Technology Data Exchange (ETDEWEB)

    Zong, Yinong; Zhang, Bin; Gu, Shenyan; Lee, Kwangkook; Zhou, Jie; Yao, Guorui; Figueiredo, Dwight; Perry, Kay; Mei, Lin; Jin, Rongsheng [Cornell; (GSHU); (Sanford-Burnham)

    2012-06-27

    Synapses are the fundamental units of neural circuits that enable complex behaviors. The neuromuscular junction (NMJ), a synapse formed between a motoneuron and a muscle fiber, has contributed greatly to understanding of the general principles of synaptogenesis as well as of neuromuscular disorders. NMJ formation requires neural agrin, a motoneuron-derived protein, which interacts with LRP4 (low-density lipoprotein receptor-related protein 4) to activate the receptor tyrosine kinase MuSK (muscle-specific kinase). However, little is known of how signals are transduced from agrin to MuSK. Here, we present the first crystal structure of an agrin-LRP4 complex, consisting of two agrin-LRP4 heterodimers. Formation of the initial binary complex requires the z8 loop that is specifically present in neuronal, but not muscle, agrin and that promotes the synergistic formation of the tetramer through two additional interfaces. We show that the tetrameric complex is essential for neuronal agrin-induced acetylcholine receptor (AChR) clustering. Collectively, these results provide new insight into the agrin-LRP4-MuSK signaling cascade and NMJ formation and represent a novel mechanism for activation of receptor tyrosine kinases.

  3. Providing culturally appropriate mental health first aid to an Aboriginal or Torres Strait Islander adolescent: development of expert consensus guidelines

    OpenAIRE

    Chalmers, Kathryn J.; Bond, Kathy S.; Jorm, Anthony F; Kelly, Claire M; Kitchener, Betty A; Williams-Tchen, AJ

    2014-01-01

    Background It is estimated that the prevalence of mental illness is higher in Aboriginal and Torres Strait Islander adolescents compared to non-Aboriginal adolescents. Despite this, only a small proportion of Aboriginal youth have contact with mental health services, possibly due to factors such as remoteness, language barriers, affordability and cultural sensitivity issues. This research aimed to develop culturally appropriate guidelines for anyone who is providing first aid to an Australian...

  4. Torres Colón Madrid – España

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    Lamela, A.

    1977-09-01

    Full Text Available The Columbus Towers have introduced a new structural system in Spain which, up to now, has only been applied in 17 cases throughout the world; we refer to the suspended structure. Due to its location, at the intersection of our important Madrid streets, and to its unusual building process, the building has attracted the attention of technicians and public alike. The much discussed difference in the cost between this procedure and conventional methods, estimated to be about 20%, is widely justified and compensated in this case, to such an extent that it can be considered as the only possible functional solution in view of the site's reduced size and irregular characteristics in addition to the great height at which the Towers were planned and the lack of space available on the ground floors. The hanging structure, supported exclusively by means of a central concrete nucleus, permitted installation of the ramps and parking space in the basement, which would have been practically impossible to carry out using traditional construction methods and it freed the ground floors from structural conditioners in the interior distribution, providing the necessary clear space to carry out a flexible and varied distribution plan, as required by the characteristics of the offices, for which the building was designed.Torres Colón han introducido en España un nuevo sistema estructural, sólo aplicado hasta el momento en 17 casos en todo el mundo: el de la estructura colgada. Por su situación, en la confluencia de cuatro importantes vías madrileñas, y por las peculiaridades de su proceso constructivo, el edificio ha atraído la atención de los técnicos y del público no especializado. La muy discutida diferencia de costes entre este procedimiento y el tradicional, estimada aproximadamente en un 10-15%, queda ampliamente justificada y compensada en este caso, hasta tal punto, que puede ser considerada como la única solución funcional posible, dada la

  5. The origin of oriented lakes in the Andean foreland, Parque Nacional Torres del Paine (Chilean Patagonia)

    Science.gov (United States)

    Gonzales, Joseph; Aydin, Atilla

    2008-05-01

    The Parque Nacional Torres Del Paine and surrounding area in the Magallanes foreland basin in Chilean Patagonia is the site for numerous lakes fed by glaciers and rivers in the Andean highlands to the west. The lakes are elongate and have conspicuously systematic orientations. We hypothesize that the origin of the oriented lakes lies in the fault system, composed of a right-lateral strike-slip fault set oriented 58° from north, a left-lateral strike-slip set oriented 87°, and a thrust fault set oriented 167°, that exists within the underlying rocks. To test this hypothesis quantitatively, we determined the shape and orientation of the lakes by fitting each lake with an ellipse of appropriate aspect ratio, and later with multiple ellipses consistent with the composite geometry of some lakes. We then examined the faults in the area in terms of their kinematics, orientation and distribution. The distribution of lake orientations showed three distinct groups which appear to correspond to the three main fault groups. For lakes fitted with multiple ellipses, the difference in means between the right-lateral, left-lateral, and thrust faults and their corresponding groups of lakes are 3.05°, 1.57°, and 5.17°. Using a Kolmogorov-Smirnov (K-S) statistical test to compare the orientations of faults with respect to the lakes suggests that there is not a strongly significant difference between the fault orientations and the corresponding lake groups. These results indicate that the faults have a profound control on the orientation, shape, and distribution of the lakes. We attribute this to faults and their damage zones being weaker and therefore prone to a faster rate of erosion, and to stress perturbations associated with discontinuous faults resulting in localized high density fracturing and surface subsidence. These results have implications for lake and drainage system morphologies in other foreland basins along the Andes and other similar settings.

  6. Estructura de las Torres Colón - Madrid – España

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    Fernández Casado, C.

    1977-09-01

    Full Text Available This article describes the general guidelines for planning the concrete structure of this unique building which was necessary in view of the site's special requirements and maximum utilization of the resulting floors. In the first place, it deals with the specific problem, which is the suspended towers and their résistance, with the basic elements being studied separately: nucleus, upper head or suspension platform, tierods, ground floors and formwork movement. In the second place, the solution adopted for the underground part is taken into consideration and finally the article explains the intermediate zone foreseen in the initial project, although not in its final scope, which is the only part which, for justified reasons, was solved using a metallic structure.Se describen en este artículo las directrices generales para la organización de la estructura de hormigón de este edificio singular, que ha venido impuesta por imperativos ineludibles de solar y máxima utilización de las plantas resultantes. En primer lugar, se trata el problema específico, que son las torres colgadas y su comportamiento resistente, estudiando por separado sus elementos constitutivos: núcleo, cabeza superior o plataforma de cuelgue, tirantes, plantas tipo y movimiento de la cimbra. En segundo lugar se plantea la solución adoptada para la parte enterrada, y finalmente, se explica la zona intermedia prevista en el proyecto inicial, aunque no con su amplitud definitiva, que es la única parte que por razones que se justifican se ha resuelto a base de estructura metálica.

  7. A Review of Programs That Targeted Environmental Determinants of Aboriginal and Torres Strait Islander Health

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    Kevin Rowley

    2013-08-01

    Full Text Available Objective: Effective interventions to improve population and individual health require environmental change as well as strategies that target individual behaviours and clinical factors. This is the basis of implementing an ecological approach to health programs and health promotion. For Aboriginal People and Torres Strait Islanders, colonisation has made the physical and social environment particularly detrimental for health. Methods and Results: We conducted a literature review to identify Aboriginal health interventions that targeted environmental determinants of health, identifying 21 different health programs. Program activities that targeted environmental determinants of health included: Caring for Country; changes to food supply and/or policy; infrastructure for physical activity; housing construction and maintenance; anti-smoking policies; increased workforce capacity; continuous quality improvement of clinical systems; petrol substitution; and income management. Targets were categorised according to Miller’s Living Systems Theory. Researchers using an Indigenous community based perspective more often identified interpersonal and community-level targets than were identified using a Western academic perspective. Conclusions: Although there are relatively few papers describing interventions that target environmental determinants of health, many of these addressed such determinants at multiple levels, consistent to some degree with an ecological approach. Interpretation of program targets sometimes differed between academic and community-based perspectives, and was limited by the type of data reported in the journal articles, highlighting the need for local Indigenous knowledge for accurate program evaluation. Implications: While an ecological approach to Indigenous health is increasingly evident in the health research literature, the design and evaluation of such programs requires a wide breadth of expertise, including local Indigenous

  8. ADECUACIÓN DEL ACCESO A LA TORRE DEL HOMENAJE DEL ALCÁZAR DE ESTEPA

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    Alfonso del Pozo y Barajas

    2011-05-01

    Full Text Available RESUMEN Esta breve investigación pretende alumbrar algunos aspectos relativos al binomio duración/alteridad desde la praxis de la Arquitectura, y lo hace a través de la crítica pormenorizada de las fases progresivas de un proyecto relativamente reciente, que se desarrolla en el ámbito de lo patrimonial: La adecuación del acceso a la Torre del Homenaje del Alcázar de Estepa. Como se verá, en el discurso se reaviva de manera intencionada una vieja cuestión disciplinar: la de la relación análisis/proyecto que, desde su primera, reductiva formulación por Saverio Muratori y Gianfranco Caniggia, las posteriores aportaciones de Carlo Aymonino y, sobre todo, de Aldo Rossi, permanece como una herida que aún no se ha cerrado.SUMMARY This brief research seeks to illuminate some aspects of the duration /otherness combination from the practice of architecture. It does so through detailed criticism of the progressive phases of a relatively recent project developed in the heritage field: Improving access to the Tower of Homage of the Alcázar of Estepa. As will be seen, an old question of the discipline is intentionally revived in the discourse: that of the analysis / planning relationship which, since its first reductive formulation by Saverio Muratori and Gianfranco Caniggia, the subsequent contributions of Carlo Aymonino and, especially, Aldo Rossi, remain a wound that has not healed.

  9. A Review of Programs That Targeted Environmental Determinants of Aboriginal and Torres Strait Islander Health

    Science.gov (United States)

    Johnston, Leah; Doyle, Joyce; Morgan, Bec; Atkinson-Briggs, Sharon; Firebrace, Bradley; Marika, Mayatili; Reilly, Rachel; Cargo, Margaret; Riley, Therese; Rowley, Kevin

    2013-01-01

    Objective: Effective interventions to improve population and individual health require environmental change as well as strategies that target individual behaviours and clinical factors. This is the basis of implementing an ecological approach to health programs and health promotion. For Aboriginal People and Torres Strait Islanders, colonisation has made the physical and social environment particularly detrimental for health. Methods and Results: We conducted a literature review to identify Aboriginal health interventions that targeted environmental determinants of health, identifying 21 different health programs. Program activities that targeted environmental determinants of health included: Caring for Country; changes to food supply and/or policy; infrastructure for physical activity; housing construction and maintenance; anti-smoking policies; increased workforce capacity; continuous quality improvement of clinical systems; petrol substitution; and income management. Targets were categorised according to Miller’s Living Systems Theory. Researchers using an Indigenous community based perspective more often identified interpersonal and community-level targets than were identified using a Western academic perspective. Conclusions: Although there are relatively few papers describing interventions that target environmental determinants of health, many of these addressed such determinants at multiple levels, consistent to some degree with an ecological approach. Interpretation of program targets sometimes differed between academic and community-based perspectives, and was limited by the type of data reported in the journal articles, highlighting the need for local Indigenous knowledge for accurate program evaluation. Implications: While an ecological approach to Indigenous health is increasingly evident in the health research literature, the design and evaluation of such programs requires a wide breadth of expertise, including local Indigenous knowledge. PMID

  10. Torre “Costa Rica” - La Coruña – España

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    Franco Taboada, J. A.

    1977-07-01

    Full Text Available The building —which reaches 106 m in height over Street level— is located on a city lot in the isthmus which geographically defines the city and is made up by two well differentiated bodies: a a 5-storey block of offices and business premises, which covers the lot from dividing wall to dividing wall; b a tower of apartments, with 24 floors and 3 apartments per floor. It also has two basement floors for parking and a ground floor for businesses. It is a singular construction for several reasons, among which emphasis must be given to its original structure a pioneer in Spain, as well as to the arrangement of the apartments —all facing the outside— and the exterior treatment with prefabricated pieces of white concrete.El edificio —que alcanza los 106 m de altura sobre la rasante de la calle— está situado en un solar urbano, en el istmo que define geográficamente la ciudad, y se c o m p o n e de dos cuerpos bien diferenciados: a Bloque de oficinas y locales comerciales, de cinco plantas, que cubre las medianerías del solar. b Torre de viviendas, con 24 alturas y tres viviendas por planta. Dispone además de dos sótanos de garaje y una planta baja destinada a locales. Se trata de una construcción singular, por varias razones, entre las que hay que destacar su estructura original y pionera en España, si bien son dignas de mención, igualmente, la distribución de las viviendas —todas exteriores— y el tratamiento exterior, a base de piezas prefabricadas de hormigón blanco.

  11. The reflectance confocal microscopy features of sebaceous adenoma in a case of Muir Torre syndrome

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    Esma İnan Yüksel

    2015-03-01

    Full Text Available Muir-Torre syndrome (MTS is a rare autosomal dominant genodermatosis characterized by the occurrence of sebaceous gland neoplasms and/or keratoacanthomas associated with visceral malignancies. It is considered as a subtype of hereditary nonpolyposis colorectal cancer syndrome. Characteristic sebaceous gland neoplasms include sebaceous adenoma, sebaceous carcinoma, sebaceoma, and keratoacanthoma with sebaceous differentiation. The most common visceral malignancies are colorectal and genitourinary tumors. CASE: A 47year-old male patient admitted to our clinic complaining of two lesions on the nose. Dermatological examination revealed a plaque in 1 cm diameter consisting of bright yellowish-white coloured papules with slightly umblicated appearance and telangiectasias on the left site of the nose and had a dome shaped papule in 3 mm diameter with hyperkeratotic plug on the tip of the nose. He had personal history of partial colon resection because of colon cancer and familial Lynch 2 syndrome. On dermoscopic examination of sebaceous adenoma, a few yellow comedo-like globules and branching arborizing vessels were detected. Reflectance confocal microscopy (RCM revealed a good histopathologic correlation. Sebaceous lobules were composed by clusters of ovoid cells with hyporefractile dark nuclei and bright, hyperrefractile glistening cytoplasm. Numerous roundish to ovoid dark spaces corresponding to sebaceous ducts were detected. The diagnosis of MTS was established based on the personal and family history, dermoscopic, RCM and histopathologic findings. CONCLUSIONS: MTS evaluation is required in patients with biopsy-proven sebaceous adenoma. Early diagnosis may be lifesaving in patients with MTS. A better characterization of RCM features of sebaceous tumors will allow early diagnosis of the patients with MTS.

  12. Association between arsenic exposure and behavior among first-graders from Torreón, Mexico.

    Science.gov (United States)

    Roy, Aditi; Kordas, Katarzyna; Lopez, Patricia; Rosado, Jorge L; Cebrian, Mariano E; Vargas, Gonzalo Garcia; Ronquillo, Dolores; Stoltzfus, Rebecca J

    2011-07-01

    Previous studies suggest adverse effects of arsenic exposure on children's cognitive function. In this study, we examined the potential association between arsenic exposure and children's behavior. Five hundred and twenty-six children, 6-7 years old, living near a metal foundry in Torreón, Mexico, participated in the study. Arsenic exposure was measured as total urinary arsenic (UAs) and arsenic metabolites-monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) concentrations. Children's behavior was assessed by Conners Behavior Rating Scales for parents and teachers. The median (interquartile range) concentrations of UAs, MMA and DMA were 55.2 (39.7), 6.7 (5.9) and 39.3 (28.5) μg/L, respectively. The mean behavior scores from parent and teacher ratings were within the clinically normal range (T<65). The relationship between behavior and urinary arsenic was modeled in linear and logistic regression models, with UAs, MMA and DMA tested in separate models and entered as quartiles. No significant association was found between any measure of urinary arsenic and parent ratings of behavior. However, higher UAs was modestly associated with higher scores on the Oppositional, Cognitive Problems and ADHD sub-scales of the teacher ratings; a dose-response relationship was not established between UAs quartiles and behavior. Higher urinary DMA was associated with higher ratings on the Oppositional, Cognitive Problems and ADHD Index by teachers. The associations between UAs and behavior became statistically non-significant after adjustment for the Peabody Picture Vocabulary Test scores, suggesting that the harmful effects of arsenic on behavior may be secondary to arsenic-induced cognitive deficits. These data suggest a potential adverse association between arsenic and children's behavior and indicate a need to further study the effects of arsenic and arsenic metabolites on neurobehavioral outcomes in children. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. The ideas of Frantz Fanon and culturally safe practices for aboriginal and Torres Strait Islander people in Australia.

    Science.gov (United States)

    Molloy, Luke; Grootjans, John

    2014-03-01

    Mainstream mental health services in Australia have failed to provide culturally appropriate care for Aboriginal and Torres Strait Islander people despite several national reports and policies that have attempted to promote positive service development in response to the calls for change from the Aboriginal and Torres Strait Islander communities. In light of this situation, this article considers the ideas of Frantz Fanon and their potential for promoting cultural safety (Ramsden, 2002) in mainstream mental health services. This article argues that Fanon's ideas provide a conceptual strategy for nurses that prompts reflection and establishes a critical theoretical perspective linking power imbalance and inequitable social relationships in health care, thus complementing the aims of cultural safety. The purpose of this critical reflection is to guide nurses' understanding of the relationship between colonization and health status in order to change their attitudes from those that continue to support current hegemonic practices and systems of health care to those that support the health of Aboriginal and Torres Strait Islander people.

  14. Las torres del litoral en el reinado de Felipe II : una arquitectura para la defensa del territorio (II

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    Alicia Cámara Muñoz

    1991-01-01

    Full Text Available Fue a mediados del siglo XVI cuando la frecuencia de ataques a las costas de este reino hicieron necesario que la monarquía se preocupara de hallar el modo de defenderlas. En 1554 Carlos I ordenó al corregidor Ñuño del Águila hacer un informe acerca de los mejores sitios para la construcción de las torres. Al año siguiente se aprobó la construcción de una torre en el cabo de Palos y otra en el Estacio, ambas en la zona del Mar Menor. Esa zona siempre fue muy vulnerable debido a que —según cuenta fray Gerónimo Hurtado— al haber agua dulce en la Manga, allí «se proveen muchos navios, especial de los enemigos que no pueden llegar a hacer aguadas en las fuentes y ríos de nuestra costa... suelen los enemigos varar barcos por esta manga de tierra y hacer presa en la gente del campo y en pastores y otros que se van a recrear allí». En la zona de la Manga el concejo de Murcia había levantado, a partir de 1526, la torre de la Encañizada, que fue reparada tanto en 1564 como en 1578.

  15. Antioxidative and neuroprotective activities of peanut sprout extracts against oxidative stress in SK-N-SH cells

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    Pranee Lertkaeo

    2017-01-01

    Conclusions: PSE has neuroprotective activities against oxidative stress in SK-N-SH cells induced by PQ, suggesting that PSE is a highly promising agent in the prevention of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

  16. Outcomes of Single Segment Implantation of Conventional Intacs versus Intacs SK for Keratoconus

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    Mohammad Nasser Hashemian

    2014-01-01

    Full Text Available Purpose: To compare the visual, refractive, and keratometric outcomes of single-segment conventional and severe keratoconus (SK types of Intacs for correction of inferior keratoconus (KCN. Methods: A total number of 41 consecutive eyes of 23 patients with a diagnosis of inferior KCN underwent Intacs implantation. Eight eyes were excluded due to postoperative complications (4 eyes and loss to follow-up (4 eyes and finally 33 eyes underwent statistical analysis. Two groups were created according to Intacs type insertion; conventional group (17 eyes and SK groups (16 eyes. Intracorneal ring segments (ICRS implantation was indicated in keratoconic patients with contact-lens intolerance or reduced best spectacle-corrected visual acuity (BSCVA. Results: In the conventional group, mean uncorrected distance visual acuity (UCDVA improved from 0.45 ± 0.41 preoperatively to 0.69 ± 0.39 six months after surgery representing a gain of 2 Snellen lines, and in the SK group mean UCDVA changed from 0.40 ± 0.35 preoperatively to 0.58 ± 0.48 equivalent to two Snellen lines improvement 6 months after operation (P = 0.48. Mean preoperative BSCVA in the conventional group improved from 0.72 ± 0.41 to 0.86 ± 0.39 (2 lines improvement postoperatively and in the SK group improved from 0.71 ± 0.69 to 0.75 ± 0.45 (0.50 line improvement ( P = 0.29. Mean preoperative spherical equivalent (SE decreased from −4.86 ± 2.26 D to −3.57 ± 2.21 D (conventional group and from −4.20 ± 1.82 D to −3.60 ± 1.89 D (P = 0.34, mean astigmatism (AST decreased from −5.20 ± 2.07 D and −4.50 ± 2.26 D to −4.02 ± 2.57 D and − 3.18 ± 2.14 D in the conventional and SK groups, respectively (P = 0.68. Finally, mean K min decreased from 47.11 ± 2.51 D to 45.40 ± 3.30 D in the conventional group and from 45.05 ± 2.59 D to 44 ± 3.88 D in the SK group (P = 0.63 and mean K max was decreased from 52.82 ± 3.23 D to 50.52 ± 3.57 D and from 49.72 ± 3.17 D to 48.55 ± 4

  17. Using cultural immersion as the platform for teaching Aboriginal and Torres Strait Islander health in an undergraduate medical curriculum.

    Science.gov (United States)

    Smith, Janie D; Wolfe, Christina; Springer, Shannon; Martin, Mary; Togno, John; Bramstedt, Katrina A; Sargeant, Sally; Murphy, Bradley

    2015-01-01

    In 2011 Bond University was looking for innovative ways to meet the professional standards and guidelines in Aboriginal and Torres Strait Islander health in its Bachelor of Medicine, Bachelor of Surgery (MBBS) curriculum. In 2012 Bond piloted a compulsory cultural immersion program for all first year students, which is now a usual part of the MBBS program. Three phases were included - establishing an Indigenous health group, determining the Aboriginal and Torres Strait Islander educational content based on the professional standards and developing nine educational sessions and resources - as well as significant administrative processes. The cultural immersion was piloted in 2012 with 92 first year medical students. Following refinements it was repeated in 2013 with 95 students and in 2014 with 94 students. A comprehensive evaluation process was undertaken that included a paper-based evaluation form using a five-point Likert scale, as well as a confidential talking circle evaluation. The response rate was 95.4% (n=271, pooled cohort). Data were entered separately into SPSS and annual reports were written to the Faculty. Descriptive statistics are reported alongside themed qualitative data. The three combined student evaluation results were extremely positive. Students (n=271) strongly agreed that the workshop was well organised (M=4.3), that the facilitators contributed very positively to their experience (M=4.3), and that they were very satisfied overall with the activity (M=4.2). They agreed that the eight overall objectives had been well met (M=3.9-4.3). The nine sessions were highly evaluated with mean ratings of between 3.9 and 4.8. The 'best thing' about the immersion identified by more than half of the students was overwhelmingly (n=140) the Storytelling session, followed by bonding with the cohort, the Torres Strait Islander session and learning more about culture. The item identified as needing most improvement was the food (n=87), followed by the

  18. The Small-Conductance Ca2+-Activated Potassium Channel, Subtype SK3, in the Human Myometrium Is Downregulated in Early Stages of Pregnancy

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    M. Rahbek

    2013-01-01

    Full Text Available The human myometrium is mainly relaxed during pregnancy, but, up to term, contractions become more coordinated and forceful in order to initiate delivery. Small conductance Ca2+-activated K+ channels (SK channels in human myometrium have been shown to be downregulated in late pregnancy. The aim was to investigate the presence of SK2 and SK3 in the human myometrium from nonpregnant women, pregnant women at term, and pregnancies delivered preterm and, in addition, to characterize the time of downregulation of these channels. Using qRT-PCR, we observed significantly lower levels of mRNA for SK2 than for SK3 in the nonpregnant tissue. The mRNA levels of SK3 were significantly reduced in tissue from pregnancies at term and pregnancies resulting in preterm deliveries, whereas no downregulation for SK2 was observed. Western blotting confirmed the qRT-PCR results. Using immunohistochemical staining, both SK2 and SK3 were detected in endometrial glandular epithelium. We conclude that SK3 mRNA is downregulated early in pregnancy—at least among those that result in preterm deliveries. Furthermore, we find that SK channels are expressed not only in the uterine smooth muscle but also in the endometrial epithelium.

  19. The SK-N-AS human neuroblastoma cell line develops osteolytic bone metastases with increased angiogenesis and COX-2 expression

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    Takahiro Tsutsumimoto

    2014-11-01

    Full Text Available Neuroblastoma (NB, which arises from embryonic neural crest cells, is the most common extra-cranial solid tumor of childhood. Approximately half of NB patients manifest bone metastasis accompanied with bone pain, fractures and bone marrow failure, leading to disturbed quality of life and poor survival. To study the mechanism of bone metastasis of NB, we established an animal model in which intracardiac inoculation of the SK-N-AS human NB cells in nude mice developed osteolytic bone metastases with increased osteoclastogenesis. SK-N-AS cells induced the expression of receptor activator of NF-κB ligand and osteoclastogenesis in mouse bone marrow cells in the co-culture. SK-N-AS cells expressed COX-2 mRNA and produced substantial amounts of prostaglandin E2 (PGE2. In contrast, the SK-N-DZ and SK-N-FI human NB cells failed to develop bone metastases, induce osteoclastogenesis, express COX-2 mRNA and produce PGE2. Immunohistochemical examination of SK-N-AS bone metastasis and subcutaneous tumor showed strong expression of COX-2. The selective COX-2 inhibitor NS-398 inhibited PGE2 production and suppressed bone metastases with reduced osteoclastogenesis. NS-398 also inhibited subcutaneous SK-N-AS tumor development with decreased angiogenesis and vascular endothelial growth factor-A expression. Of interest, metastasis to the adrenal gland, a preferential site for NB development, was also diminished by NS-398. Our results suggest that COX2/PGE2 axis plays a critical role in the pathophysiology of osteolytic bone metastases and tumor development of the SK-NS-AS human NB. Inhibition of angiogenesis by suppressing COX-2/PGE2 may be an effective therapeutic approach for children with NB.

  20. Identification of Staphylococcus aureus Cellular Pathways Affected by the Stilbenoid Lead Drug SK-03-92 Using a Microarray.

    Science.gov (United States)

    Schwan, William R; Polanowski, Rebecca; Dunman, Paul M; Medina-Bielski, Sara; Lane, Michelle; Rott, Marc; Lipker, Lauren; Wescott, Amy; Monte, Aaron; Cook, James M; Baumann, Douglas D; Tiruveedhula, V V N Phani Babu; Witzigmann, Christopher M; Mikel, Cassandra; Rahman, Md Toufiqur

    2017-09-11

    The mechanism of action for a new lead stilbene compound coded SK-03-92 with bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA) is unknown. To gain insight into the killing process, transcriptional profiling was performed on SK-03-92 treated vs. untreated S. aureus. Fourteen genes were upregulated and 38 genes downregulated by SK-03-92 treatment. Genes involved in sortase A production, protein metabolism, and transcriptional regulation were upregulated, whereas genes encoding transporters, purine synthesis proteins, and a putative two-component system (SACOL2360 (MW2284) and SACOL2361 (MW2285)) were downregulated by SK-03-92 treatment. Quantitative real-time polymerase chain reaction analyses validated upregulation of srtA and tdk as well as downregulation of the MW2284/MW2285 and purine biosynthesis genes in the drug-treated population. A quantitative real-time polymerase chain reaction analysis of MW2284 and MW2285 mutants compared to wild-type cells demonstrated that the srtA gene was upregulated by both putative two-component regulatory gene mutants compared to the wild-type strain. Using a transcription profiling technique, we have identified several cellular pathways regulated by SK-03-92 treatment, including a putative two-component system that may regulate srtA and other genes that could be tied to the SK-03-92 mechanism of action, biofilm formation, and drug persisters.

  1. Possible mechanism of leptin on human SK-BR-3 breast cancer cells%瘦素作用于人乳腺癌SK-BR-3细胞的可能机制

    Institute of Scientific and Technical Information of China (English)

    曾繁余; 刘菁; 黄颖; 骆耐香

    2013-01-01

    Objective To explore the possible mechanism of leptin on human SK-BR-3 breast cancer cells, cell proliferation and vascular endothelial growth factor ( VEGF) expression. Methods SK-BR-3 cells were treated with different concentration of leptin for different hours in vitro, respectively. Cell proliferation was evaluated by CCK-8 method. The expression of VEGF mRNA and protein were detected in SK-BR-3 by real time PCR and western blot, respectively. Results Cell proliferation of SK-BR-3 was in a dose and time-dependent way in vitro (P < 0. 05 ). The expression level of VEGF mRNA and protein were increased significantly in a leptin dose-dependent manner ( P < 0. 05 ). Conclusions Leptin promotes the growth of human SK-BR-3 breast cancer cells in vitro, which plays a role in the mechanism of angiogenesis and metastasis by increasing the expression of VEGF.%目的 观察瘦素对人乳腺癌SK-BR-3细胞增殖及对VEGF表达的影响,探讨其可能机制.方法 不同浓度瘦素与人乳腺癌SK-BR-3细胞共同培养后,CCK-8法检测其对人乳腺癌SK-BR-3细胞增殖的影响,实时荧光定量PCR法及Western印迹法检测VEGF mRNA及蛋白表达情况.结果 在一定范围内,瘦素呈时间、剂量依赖性促进SK-BR-3细胞的生长(P<0.05);细胞受不同浓度瘦素作用后VEGF mRNA及蛋白表达量明显增加并具有剂量依赖性(P<0.05).结论 在体外瘦素对人乳腺癌SK-BR-3细胞有明显增殖作用,通过促进VEGF的表达,在促进血管的生成及肿瘤转移机制中可能发挥一定作用.

  2. Effects of PDGF-BB on migration and proliferation of SK-BR-3 mammary carcinoma cells%PDGF-BB 对 SK-BR-3乳腺癌细胞迁移、增殖的影响

    Institute of Scientific and Technical Information of China (English)

    步玉辉; 史建红; 崔乃鹏; 何欢; 王冰; 陈保平

    2014-01-01

    目的:观察血小板源性生长因子(PDGF)-BB对乳腺癌SK-BR-3细胞迁移、增殖的促进作用。方法分别以0.5、1、2、4ng/mL质量浓度的PDGF-BB干预对数期SK-BR-3乳腺癌细胞,细胞计数法和內盐法(MTS)检测细胞增殖情况,细胞迁移试验检测细胞迁移能力。结果与未加入任何药物的SK-BR-3乳腺癌细胞相比,4ng/mL的PDGF-BB对SK-BR-3乳腺癌细胞的迁移及增殖有明显促进作用,该促进作用与药物质量浓度呈正相关。结论在一定质量浓度范围内,PDGF-BB能明显促进人源性SK-BR-3细胞的迁移和增殖。%Objective To explore the effects of platelet-derived growth factor ( PDGF)-BB on the migration and prolif-eration of SK-BR-3 mammary carcinoma cells .Methods SK-BR-3 mammary carcinoma cells in logarithm growth stage were treated with different concentrations of PDGF-BB (0.5, 1, 2, 4 ng/mL).The proliferation was determined by cytom-etry and MTS assay , and the migration ability was detected by cell migration assay .Results Compared with SK-BR-3 mammary carcinoma cells without any drug , 4 ng/mL PDGF-BB promoted the migration and proliferation of SK-BR-3 mam-mary cells and it was positively associated with the drug concentration .Conclusions The PDGF-BB can promote the mi-gration and proliferation of human SK-BR-3 cells in a specific concentration range .

  3. Validation of risk assessment scales and predictors of intentions to quit smoking in Australian Aboriginal and Torres Strait Islander peoples: a cross-sectional survey protocol

    OpenAIRE

    Gould, Gillian Sandra; Watt, Kerrianne; McEwen, Andy; Cadet-James, Yvonne; Clough, Alan R

    2014-01-01

    Introduction Tobacco smoking is a very significant behavioural risk factor for the health of Australian Aboriginal and Torres Strait Islanders, and is embedded as a social norm. With a focus on women of childbearing age, and men of similar age, this project aims to determine how Aboriginal and Torres Strait Islander smokers assess smoking risks and how these assessments contribute to their intentions to quit. The findings from this pragmatic study should contribute to developing culturally ta...

  4. Social determinants and psychological distress among Aboriginal and Torres Strait islander adults in the Australian state of Victoria: a cross-sectional population based study.

    Science.gov (United States)

    Markwick, Alison; Ansari, Zahid; Sullivan, Mary; McNeil, John

    2015-03-01

    Aboriginal and Torres Strait Islander adults in the Australian state of Victoria have a higher prevalence of psychological distress than their non-Aboriginal and Torres Strait Islander counterparts. We sought to explain this inequality, focussing on the social determinants of health. We used population-based survey data from the 2008 Victorian Population Health Survey; a cross-sectional landline computer-assisted telephone survey of 34,168 randomly selected adults. We defined psychological distress as a score of 22 or more on the Kessler 10 Psychological Distress scale. We used logistic regression to identify socio-demographic characteristics and social capital indicators that were associated with psychological distress. We then created multivariable models to explore the association between psychological distress and Aboriginal and Torres Strait Islander status that incorporated all significant socioeconomic status (SES) and social capital variables, adjusting for all non-SES socio-demographic characteristics. Aboriginal and Torres Strait Islander Victorians (24.5%) were more than twice as likely than their non-Aboriginal and Torres Strait Islander counterparts (11.3%) to have psychological distress (odds ratio (OR) = 2.56, 95% confidence interval; 1.67-3.93). Controlling for SES, negative perceptions of the residential neighbourhood, lack of social support from family, social and civic distrust, and all non-SES socio-demographic variables (age, sex, marital status, household composition, and rurality), rendered the previously statistically significant inequality in the prevalence of psychological distress, between Aboriginal and Torres Strait Islander Victorians and their non-Aboriginal and Torres Strait Islander counterparts, insignificant at the p = 0.05 level (OR = 1.50; 0.97-2.32). Psychological distress is an important health risk factor for Aboriginal and Torres Strait Islander adults that has yet to be widely acknowledged and addressed. Addressing the

  5. Tres Torres y un mar: La última obra de Fernando Cassinello

    Directory of Open Access Journals (Sweden)

    García-Sánchez, J. F.

    2014-12-01

    Full Text Available The work of spanish architect, Fernando Cassinello Pérez has been rarely published, although he built a lot and well. His life is based on three areas: architectural production, academic and research activities. In this research his latest work will be discussed: Three Tower triplets Star-Building (1975 was built on the seafront in Zapillo, Almería; and that is a smart residential work of formal investigation and structural sincerity. Star-Building is an example of how, since the resignation and moderate density, it is possible to construct interesting proposals for housing -oriented towards tourism- on the beachfront. Of course, it deserves to be well known by who nowadays decides to design and build collective architecture. The three project versions will be discussed and described, also explaining how the structure and the traces of the process of building are the genesis and story of this work.La obra del arquitecto almeriense Fernando Cassinello Pérez ha sido escasamente publicada, pero lo cierto es que construyó mucho y bien. Su vida descansa sobre tres pilares: la producción arquitectónica, la actividad académica y la inquietud investigadora. Aquí se expondrá su última obra: las Tres Torres trillizas Star-Building (1975 que construyó frente al Paseo Marítimo de Almería, en el barrio de El Zapillo; y que son un elegante ejercicio residencial de investigación formal y sinceridad estructural. También son un ejemplo de cómo, desde la renuncia y con una densidad moderada, es posible construir interesantes propuestas de viviendas –orientadas al turismo– en la primera línea de playa. Y que, desde luego, merecen ser conocida por quien decida hoy proyectar y construir arquitectura colectiva. Se analizarán y describirán las tres versiones del proyecto; y se explicará cómo la estructura y las huellas del proceso de construcción son la génesis y el argumento de esta obra.

  6. Leczenie pierwotnych chłoniaków skóry. Rekomendacje Sekcji Chłoniaków Skóry Polskiej Grupy Badawczej Chłoniaków (PLRG

    Directory of Open Access Journals (Sweden)

    Jan Walewski

    2010-09-01

    Full Text Available Pierwotne chłoniaki skóry stanowią heterogenną grupę nowotworówukładu limfoidalnego, wśród których około 75% wywodzi się z limfocytówT i których pierwotnym i dominującym klinicznie umiejscowieniemjest skóra. Pierwotne chłoniaki skóry cechuje odmienny przebiegkliniczny i rokowanie w porównaniu z chłoniakami układowymi.Chłoniaki skóry mają najczęściej przebieg przewlekły, ale całkowitewyleczenie chorego rzadko jest możliwe. Diagnostyka i leczenie pierwotnychchłoniaków skóry jest zagadnieniem interdyscyplinarnym,w które powinni być zaangażowani, obok dermatologów, równieżonkolodzy, hematolodzy i patomorfolodzy. Powstała w 2009 roku SekcjaChłoniaków Skóry Polskiej Grupy Badawczej Chłoniaków podjęłapróbę opracowania jednolitych zaleceń terapeutycznych, które byłybyuznane i stosowane w środowiskach wszystkich zaangażowanych specjalności.Są one oparte na dostępnych europejskich i światowych rekomendacjachoraz na doświadczeniu polskich ośrodków dermatologicznychi hematoonkologicznych w zakresie leczenia chłoniaków skóry.

  7. Effect of Trastuzumab on Notch-1 Signaling Pathway in Breast Cancer SK-BR3 Cells

    Institute of Scientific and Technical Information of China (English)

    Ming Han; Hua-yu Deng; Rong Jiang

    2012-01-01

    Objective:To investigate the effects and mechanisms of trastuzumab on Notch-1 pathway in breast cancer cells,recognizing the significance of Notch-1 signaling pathway in trastuzumab resistance.Methods:Immunocytochemistry staining and Western blotting were employed to justify the expression of Notch-1 protein in HER2-overexpressing SK-BR3 cells and HER2-non-overexpressing breast cancer MDA-MB-231 cells.Western blotting and reverse transcription PCR (RT-PCR) were used to detect the activated Notch-1 and Notch-1 target gene HES-1 mRNA expression after SK-BR3 cells were treated with trastuzumab.Double immunofluorescence staining and co-immunoprecipitation were used to analyze the relationship of Notch-1 and HER2 proteins.Results:The level of Notch-1 nuclear localization and activated Notch-1 proteins in HER2-overexpressing cells were significantly lower than in HER2-non-overexpressing cells (P<0.01),and the expressions of activated Notch-1 and HES-1 mRNA were obviously increased after trastuzumab treatment (P<1.05),but HER2 expression did not change significantly for trastuzumab treating (P>0.05).Moreover,Notch-1 was discovered to co-localize and interact with HER2 in SK-BR3 cells.Conclusion:Overexpression of HER2 decreased Notch-1 activity by the formation of a HER2-Notch1 complex,and trastuzumab can restore the activity of Notch-1 signaling pathway,which could be associated with cell resistance to trastuzumab.

  8. How does ethanol induce apoptotic cell death of SK-N-SH neuroblastoma cells?*

    Institute of Scientific and Technical Information of China (English)

    Yong Moon; Yongil Kwon; Shun Yu

    2013-01-01

    A body of evidence suggests that ethanol can lead to damage of neuronal cel s. However, the me-chanism underlying the ethanol-induced damage of neuronal cel s remains unclear. The role of mi-togen-activated protein kinases in ethanol-induced damage was investigated in SK-N-SH neurob-lastoma cel s. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cel viability assay, DNA fragmentation detection, and flow cytometric analysis showed that ethanol induced apoptotic cel death and cel cycle arrest, characterized by increased caspase-3 activity, DNA fragmentation, nuclear disruption, and G 1 arrest of cel cycle of the SK-N-SH neuroblastoma cel s. In addition, western blot analysis indicated that ethanol induced a lasting increase in c-Jun N-terminal protein kinase activity and a transient increase in p38 kinase activity of the neuroblastoma cel s. c-Jun N-terminal protein kinase or p38 kinase inhibitors significantly reduced the ethanol-induced cel death. Ethanol also increased p53 phosphorylation, fol owed by an increase in p21 tumor sup-pressor protein and a decrease in phospho-Rb (retinoblastoma) protein, leading to alterations in the expressions and activity of cyclin dependent protein kinases. Our results suggest that ethanol me-diates apoptosis of SK-N-SH neuroblastoma cel s by activating p53-related cel cycle arrest possibly through activation of the c-Jun N-terminal protein kinase-related cel death pathway.

  9. Northern settlement family-style: labor planning and population policy in Noril'sk.

    Science.gov (United States)

    Bond, A R

    1985-01-01

    This paper reviews labor planning and population policies in Noril'sk, a mining and metallurgical settlement in northern Siberia. When the settlement was established in 1935, planners were lacking in ideas about how to recruit workers to staff the mines and smelters and how to retain the labor force once it was in place. From 1935-79, planners followed a forced labor policy dependent upon the labor of prisoners. However, this solution was not adequate for meeting the manpower needs of an economy undergoing rapid technological sophistication. Northern wage increments were introduced after 1945 to recruit skilled workers from other regions. These increments built up over a 4-year period to a maximum of 80% of base pay. A special cost of living bonus was also awarded. Although these inducements facilitated labor recruitment, labor retention remained a major problem. Surveys indicated that workers would prefer improvements in housing and social services to further wage increases. Thus, policy was directed at the housing shortages, poor medical care, and inadequate child care facilities in Noril'sk. Such improvements facilitated labor retention but also contributed to overpopulation. The population doubled between 1956-80, exceeding 180,000 in the latter year. In the early 1980s, selective measures toward population control were implemented to ensure maintenance of living standards (e.g., encouragement of older workers to leave the area upon retirement, more careful screening of recruits). The goal is to stabilize city size at around 250,000. The Noril'sk case illustrates that quality of life investments can alleviate labor retention problems even in the harshest physical environments. Recent policies have advocated productivity-enriching technologies that do not require increments in the labor force.

  10. Food truck som ett nytt fenomen i Skåne

    OpenAIRE

    Fromell, Hilda; Winter, Regina

    2016-01-01

    Food truck is a mobile restaurant business which originates from the United States, and can now be seen also in Sweden. There are specific guidelines regarding the food that are served from the food trucks rolling the streets of Skåne from 2013. For example the food should be ecological, which is in keeping with the other municipal goals regarding food and meal. The concept is communicated and marketed through social media, which makes it possible to effectively reach many people. Four food t...

  11. Aplikace marketingu na trhu rybářských potřeb

    OpenAIRE

    Skalický, Karel

    2009-01-01

    This bachelor thesis focuses on the marketing mixture of the fishing tackle. The aim of this thesis is to introduce the market of these particular products and its marketing specifics and to get the general idea of the marketing activities of the company Rybářské centrum s.r.o. Another aim is also to recommend and suggest new marketing strategies that help to develop the company. The theoretical part deals with all the important terminology concerning the marketing and marketing mix. The fish...

  12. Inclusive K0SK0S resonance production in ep collisions at HERA

    CERN Document Server

    AUTHOR|(CDS)2076592; Magill, S.; Musgrave, B.; Nicholass, D.; Repond, J.; Yoshida, R.; Mattingly, M.C.K.; Antonioli, P.; Bari, G.; Bellagamba, L.; Boscherini, D.; Bruni, A.; Bruni, G.; Cindolo, F.; Corradi, M.; Iacobucci, G.; Margotti, A.; Nania, R.; Polini, A.; Antonelli, S.; Basile, M.; Bindi, M.; Cifarelli, L.; Contin, A.; De Pasquale, S.; Sartorelli, G.; Zichichi, A.; Bartsch, D.; Brock, I.; Hartmann, H.; Hilger, E.; Jakob, H.P.; Jungst, M.; Nuncio-Quiroz, A.E.; Paul, E.; Samson, U.; Schonberg, V.; Shehzadi, R.; Wlasenko, M.; Brook, N.H.; Heath, G.P.; Morris, J.D.; Capua, M.; Fazio, S.; Mastroberardino, A.; Schioppa, M.; Susinno, G.; Tassi, E.; Kim, J.Y.; Ibrahim, Z.A.; Kamaluddin, B.; Wan Abdullah, W.A.T.; Ning, Y.; Ren, Z.; Sciulli, F.; Chwastowski, J.; Eskreys, A.; Figiel, J.; Galas, A.; Gil, M.; Olkiewicz, K.; Stopa, P.; Zawiejski, L.; Adamczyk, L.; Bold, T.; Grabowska-Bold, I.; Kisielewska, D.; Lukasik, J.; Przybycien, M.; Suszycki, L.; Kotanski, A.; Slominski, W.; Behrens, U.; Blohm, C.; Bonato, A.; Borras, K.; Ciesielski, R.; Coppola, N.; Fang, S.; Fourletova, J.; Geiser, A.; Gottlicher, P.; Grebenyuk, J.; Gregor, I.; Haas, T.; Hain, W.; Huttmann, A.; Januschek, F.; Kahle, B.; Katkov, I.I.; Klein, U.; Kotz, U.; Kowalski, H.; Lobodzinska, E.; Lohr, B.; Mankel, R.; Melzer-Pellmann, I.A.; Miglioranzi, S.; Montanari, A.; Namsoo, T.; Notz, D.; Parenti, A.; Rinaldi, L.; Roloff, P.; Rubinsky, I.; Santamarta, R.; Schneekloth, U.; Spiridonov, A.; Szuba, D.; Szuba, J.; Theedt, T.; Wolf, G.; Wrona, K.; Yagues Molina, A.G.; Youngman, C.; Zeuner, W.; Drugakov, V.; Lohmann, W.; Schlenstedt, S.; Barbagli, G.; Gallo, E.; Pelfer, P.G.; Bamberger, A.; Dobur, D.; Karstens, F.; Vlasov, N.N.; Bussey, P.J.; Doyle, A.T.; Dunne, W.; Forrest, M.; Rosin, M.; Saxon, D.H.; Skillicorn, I.O.; Gialas, I.; Papageorgiu, K.; Holm, U.; Klanner, R.; Lohrmann, E.; Schleper, P.; Schorner-Sadenius, T.; Sztuk, J.; Stadie, H.; Turcato, M.; Foudas, C.; Fry, C.; Long, K.R.; Tapper, A.D.; Matsumoto, T.; Nagano, K.; Tokushuku, K.; Yamada, S.; Yamazaki, Y.; Barakbaev, A.N.; Boos, E.G.; Pokrovskiy, N.S.; Zhautykov, B.O.; Aushev, V.; Borodin, M.; Kadenko, I.; Kozulia, A.; Libov, V.; Lisovyi, M.; Lontkovskyi, D.; Makarenko, I.; Sorokin, Iu.; Verbytskyi, A.; Volynets, O.; Son, D.; de Favereau, J.; Piotrzkowski, K.; Barreiro, F.; Glasman, C.; Jimenez, M.; Labarga, L.; del Peso, J.; Ron, E.; Soares, M.; Terron, J.; Zambrana, M.; Corriveau, F.; Liu, C.; Schwartz, J.; Walsh, R.; Zhou, C.; Tsurugai, T.; Antonov, A.; Dolgoshein, B.A.; Gladkov, D.; Sosnovtsev, V.; Stifutkin, A.; Suchkov, S.; Dementiev, R.K.; Ermolov, P.F.; Gladilin, L.K.; Golubkov, Yu.A.; Khein, L.A.; Korzhavina, I.A.; Kuzmin, V.A.; Levchenko, B.B.; Lukina, O.Yu.; Proskuryakov, A.S.; Shcheglova, L.M.; Zotkin, D.S.; Abt, I.; Caldwell, A.; Kollar, D.; Reisert, B.; Schmidke, W.B.; Grigorescu, G.; Keramidas, A.; Koffeman, E.; Kooijman, P.; Pellegrino, A.; Tiecke, H.; Vazquez Acosta, Monica Luisa; Wiggers, L.; Brummer, N.; Bylsma, B.; Durkin, L.S.; Lee, A.; Ling, T.Y.; Allfrey, Philip Daniel; Bell, M.A.; Cooper-Sarkar, A.M.; Devenish, R.C.E.; Ferrando, J.; Foster, B.; Korcsak-Gorzo, K.; Oliver, K.; Robertson, A.; Uribe-Estrada, C.; Walczak, R.; Bertolin, A.; Dal Corso, F.; Dusini, S.; Longhin, A.; Stanco, L.; Bellan, P.; Brugnera, R.; Carlin, R.; Garfagnini, A.; Limentani, S.; Oh, B.Y.; Raval, A.; Ukleja, J.; Whitmore, J.J.; Iga, Y.; D'Agostini, G.; Marini, G.; Nigro, A.; Cole, J.E.; Hart, J.C.; Abramowicz, H.; Ingbir, R.; Kananov, S.; Levy, A.; Stern, A.; Kuze, M.; Maeda, J.; Hori, R.; Kagawa, S.; Okazaki, N.; Shimizu, S.; Tawara, T.; Hamatsu, R.; Kaji, H.; Kitamura, S.; Ota, O.; Ri, Y.D.; Costa, M.; Ferrero, M.I.; Monaco, V.; Sacchi, R.; Solano, A.; Arneodo, M.; Ruspa, M.; Fourletov, S.; Martin, J.F.; Stewart, T.P.; Boutle, S.K.; Butterworth, J.M.; Gwenlan, C.; Jones, T.W.; Loizides, J.H.; Wing, M.; Brzozowska, B.; Ciborowski, J.; Grzelak, G.; Kulinski, P.; Luzniak, P.; Malka, J.; Nowak, R.J.; Pawlak, J.M.; Tymieniecka, T.; Ukleja, A.; zarnecki, A.F.; Adamus, M.; Plucinski, P.; Eisenberg, Y.; Hochman, D.; Karshon, U.; Brownson, E.; Danielson, T.; Everett, A.; Kcira, D.; Reeder, D.D.; Ryan, P.; Savin, A.A.; Smith, W.H.; Wolfe, H.; Bhadra, S.; Catterall, C.D.; Cui, Y.; Hartner, G.; Menary, S.; Noor, U.; Standage, J.; Whyte, J.

    2008-01-01

    Inclusive K0SK0S production in ep collisions at HERA was studied with the ZEUS detector using an integrated luminosity of 0.5 fb-1. Enhancements in the mass spectrum were observed and are attributed to the production of f2(1270)/a02(1320), f'2(1525) and f0(1710). The f0(1710) state was observed with a statistical significance of 5 standard deviations. Masses and widths were obtained using a fit which takes into account theoretical predictions based on SU(3) symmetry arguments.

  13. Altered active zones, vesicle pools, nerve terminal conductivity, and morphology during experimental MuSK myasthenia gravis.

    Science.gov (United States)

    Patel, Vishwendra; Oh, Anne; Voit, Antanina; Sultatos, Lester G; Babu, Gopal J; Wilson, Brenda A; Ho, Mengfei; McArdle, Joseph J

    2014-01-01

    Recent studies demonstrate reduced motor-nerve function during autoimmune muscle-specific tyrosine kinase (MuSK) myasthenia gravis (MG). To further understand the basis of motor-nerve dysfunction during MuSK-MG, we immunized female C57/B6 mice with purified rat MuSK ectodomain. Nerve-muscle preparations were dissected and neuromuscular junctions (NMJs) studied electrophysiologically, morphologically, and biochemically. While all mice produced antibodies to MuSK, only 40% developed respiratory muscle weakness. In vitro study of respiratory nerve-muscle preparations isolated from these affected mice revealed that 78% of NMJs produced endplate currents (EPCs) with significantly reduced quantal content, although potentiation and depression at 50 Hz remained qualitatively normal. EPC and mEPC amplitude variability indicated significantly reduced number of vesicle-release sites (active zones) and reduced probability of vesicle release. The readily releasable vesicle pool size and the frequency of large amplitude mEPCs also declined. The remaining NMJs had intermittent (4%) or complete (18%) failure of neurotransmitter release in response to 50 Hz nerve stimulation, presumably due to blocked action potential entry into the nerve terminal, which may arise from nerve terminal swelling and thinning. Since MuSK-MG-affected muscles do not express the AChR γ subunit, the observed prolongation of EPC decay time was not due to inactivity-induced expression of embryonic acetylcholine receptor, but rather to reduced catalytic activity of acetylcholinesterase. Muscle protein levels of MuSK did not change. These findings provide novel insight into the pathophysiology of autoimmune MuSK-MG.

  14. Altered active zones, vesicle pools, nerve terminal conductivity, and morphology during experimental MuSK myasthenia gravis.

    Directory of Open Access Journals (Sweden)

    Vishwendra Patel

    Full Text Available Recent studies demonstrate reduced motor-nerve function during autoimmune muscle-specific tyrosine kinase (MuSK myasthenia gravis (MG. To further understand the basis of motor-nerve dysfunction during MuSK-MG, we immunized female C57/B6 mice with purified rat MuSK ectodomain. Nerve-muscle preparations were dissected and neuromuscular junctions (NMJs studied electrophysiologically, morphologically, and biochemically. While all mice produced antibodies to MuSK, only 40% developed respiratory muscle weakness. In vitro study of respiratory nerve-muscle preparations isolated from these affected mice revealed that 78% of NMJs produced endplate currents (EPCs with significantly reduced quantal content, although potentiation and depression at 50 Hz remained qualitatively normal. EPC and mEPC amplitude variability indicated significantly reduced number of vesicle-release sites (active zones and reduced probability of vesicle release. The readily releasable vesicle pool size and the frequency of large amplitude mEPCs also declined. The remaining NMJs had intermittent (4% or complete (18% failure of neurotransmitter release in response to 50 Hz nerve stimulation, presumably due to blocked action potential entry into the nerve terminal, which may arise from nerve terminal swelling and thinning. Since MuSK-MG-affected muscles do not express the AChR γ subunit, the observed prolongation of EPC decay time was not due to inactivity-induced expression of embryonic acetylcholine receptor, but rather to reduced catalytic activity of acetylcholinesterase. Muscle protein levels of MuSK did not change. These findings provide novel insight into the pathophysiology of autoimmune MuSK-MG.

  15. Spatially controlled Fe and Si isotope variations: an alternative view on the formation of the Torres del Paine pluton

    Science.gov (United States)

    Gajos, Norbert A.; Lundstrom, Craig C.; Taylor, Alexander H.

    2016-11-01

    We present new Fe and Si isotope ratio data for the Torres del Paine igneous complex in southern Chile. The multi-composition pluton consists of an approximately 1 km vertical exposure of homogenous granite overlying a contemporaneous 250-m-thick mafic gabbro suite. This first-of-its-kind spatially dependent Fe and Si isotope investigation of a convergent margin-related pluton aims to understand the nature of granite and silicic igneous rock formation. Results collected by MC-ICP-MS show a trend of increasing δ56Fe and δ30Si with increasing silica content as well as a systematic increase in δ56Fe away from the mafic base of the pluton. The marginal Torres del Paine granites have heavier Fe isotope signatures (δ56Fe = +0.25 ± 0.02 2se) compared to granites found in the interior pluton (δ56Fe = +0.17 ± 0.02 2se). Cerro Toro country rock values are isotopically light in both Fe and Si isotopic systems (δ56Fe = +0.05 ± 0.02 ‰; δ30Si = -0.38 ± 0.07 ‰). The variations in the Fe and Si isotopic data cannot be accounted for by local assimilation of the wall rocks, in situ fractional crystallization, late-stage fluid exsolution or some combination of these processes. Instead, we conclude that thermal diffusion or source magma variation is the most likely process producing Fe isotope ratio variations in the Torres del Paine pluton.

  16. 相依误差线性模型中的主成分 s-K 估计%Principal Components s-K Class Estimator in the Linear Model with Correlated Errors

    Institute of Scientific and Technical Information of China (English)

    周玲; 何道江

    2015-01-01

    为同时克服线性回归模型的自相关性和回归变量间的复共线性,通过融合主成分回归估计和 s-K 估计,提出一类新估计,称为主成分 s-K 估计;并在均方误差阵意义下,得到了这类估计分别优于广义最小二乘估计、主成分估计、r-k 和 s-K 估计的充要条件。 Monto Carlo 数值模拟表明,新估计是一种同时克服自相关性和复共线性的有效方法。%To combat autocorrelation in errors and multicollinearity among the regressors in linear regression model,we proposed a new estimator by combining the principal components regression (PCR)estimator and the s-K estimator.Then necessary and sufficient conditions for the superiority of the new estimator over the GLS,the PCR,the r-k and the s-K estimators were derived by the mean squared error matrix criterion.Finally,a Monte Carlo simulation study was carried out to investigate the performance of the proposed estimator.

  17. A propósito de un gran economista colonial, Don Antonio de Narváez y La Torre

    Directory of Open Access Journals (Sweden)

    Sergio Elías Ortiz

    1962-09-01

    Full Text Available Al preclaro nombre del Precursor don Pedro Fermín de Vargas, señalado como "nuestro primer economista" en reciente artículo publicado en el Boletín Cultural y Bibliográfico, bajo la firma de Alberto Miramón, tenemos que agregar otro, no menos brillante en nuestro concepto: el del Mariscal de Campo don Antonio de Narváez y La Torre, autor de la Relación o Informe de la Provincia de Santa Marta y Río Hacha.

  18. Torre y refugio: TOTO MA Gallery, Tokio, Japón XII Bienal de Arquitectura, Venecia, Italia

    OpenAIRE

    Radic,Smiljan; Correa,Marcela

    2011-01-01

    Dos experiencias de proyecto aparecen aquí relacionadas por un antiguo relato de los hermanos Grimm. El prototipo estructural de una torre armada con copas de cristal, tensores y barras de acero inoxidable, preparado para la exposición Global ends en Tokio, es uno de los extremos narrativos. Opuesto a él, una cavidad de granito y cedro -presentada en la Muestra central de la Bienal de Venecia en 2010- propone un espacio en penumbra, silencioso, fragante y seguro, a medio camino entre escondit...

  19. Trail impacts and trail impact management related to ecotourism visitation at Torres del Paine National Park, Chile

    Science.gov (United States)

    Farrell, T.A.; Marion, J.L.

    2002-01-01

    Ecotourism and protected area visitation in Central and South America are largely dependent upon a relatively undisturbed quality of natural resources. However, visitation may impact vegetation, soil, water and wildlife resources, and degrade visitor facilities such as recreation sites and trails. Findings are reported from trail impact research conducted at Torres del Paine National Park in Patagonia, Chile. The frequency and magnitude of selected trail impacts and the relative effect of the amount of use, vegetation type, trail position and trail grade are investigated. Findings differed from previous studies in that amount of use was significantly related to both trail width increases and trail erosion. Management actions to minimize trail impacts are offered.

  20. Torre para comunicaciones y prácticas de bomberos en la Eliana, Valencia. España

    Directory of Open Access Journals (Sweden)

    Monfort Lleonart, José

    1992-06-01

    Full Text Available The article brings the description of the characteristics of the project and the execution of the work on a communication tower which should also serve for practices of fire brigades and which has a water tank in the base.

    Se describen las características de proyecto y ejecución de una torre de comunicaciones, que además debe servir para realizar prácticas de bomberos y que alberga en su base un depósito de agua.

  1. Clinical findings in MuSK-antibody positive myasthenia gravis: a U.S. experience.

    Science.gov (United States)

    Pasnoor, Mamatha; Wolfe, Gil I; Nations, Sharon; Trivedi, Jaya; Barohn, Richard J; Herbelin, Laura; McVey, April; Dimachkie, Mazen; Kissel, John; Walsh, Ronan; Amato, Anthony; Mozaffar, Tahseen; Hungs, Marcel; Chui, Luis; Goldstein, Jonathan; Novella, Steven; Burns, Ted; Phillips, Lawrence; Claussen, Gwendolyn; Young, Angela; Bertorini, Tulio; Oh, Shin

    2010-03-01

    We performed a retrospective chart review on 53 muscle-specific kinase antibody (MuSK-Ab)-positive myasthenia gravis (MG) patients at nine university-based centers in the U.S. Of these, 66% were Caucasian, 85% were women, and age of onset was 9-79 years. Twenty-seven patients were nonresponsive to anticholinesterase therapy. Myasthenia Gravis Foundation of America improvement status was achieved in 53% patients on corticosteroids, 51% with plasma exchange, and in 20% on intravenous immunoglobulin (IVIG). Thymectomy was beneficial in 7/18 patients at 3 years. Long-term (> or =3 years) outcome was very favorable in 58% of patients who achieved remission and/or minimal manifestation status. Overall, 73% improved. There was one MG-related death. This survey reinforces several cardinal features of MuSK-Ab-positive MG, including prominent bulbar involvement and anticholinesterase nonresponsiveness. Facial or tongue atrophy was rare. Most patients respond favorably to immunotherapy. The best clinical response was to corticosteroids and plasma exchange, and the poorest response was to IVIG. Long-term outcome is favorable in about 60% of cases.

  2. A dehydrin-dehydrin interaction: the case of SK3 from Opuntia streptacantha

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    Itzell eHernandez

    2014-10-01

    Full Text Available Dehydrins belongs to a large group of highly hydrophilic proteins known as Late Embryogenesis Abundant (LEA proteins. It is well know that dehydrins are intrinsically disordered plant proteins that accumulate during the late stages of embryogenesis and in response to abiotic stresses; however, the molecular mechanisms by which their functions are carried out are still unclear. We have previously reported that transgenic Arabidopsis plants overexpressing an Opuntia streptacantha SK3 dehydrin (OpsDHN1 show enhanced tolerance to freezing stress. Herein, we show using a split-ubiquitin yeast two-hybrid system that OpsDHN1 dimerizes. We found that the deletion of regions containing K-segments and the histidine-rich region in the OpsDHN1 protein affects dimer formation. Not surprisingly, in silico protein sequence analysis suggests that OpsDHN1 is an intrinsically disordered protein, an observation that was confirmed by circular dichroism and gel filtration of the recombinantly expressed protein. The addition of zinc triggered the association of recombinantly expressed OpsDHN1 protein, likely through its histidine-rich motif. These data brings new insights about the molecular mechanism of the OpsDHN1 SK3-dehydrin

  3. Production and antioxidant activity of intracellular polysaccharide by Hypsizigus marmoreus SK-01

    Directory of Open Access Journals (Sweden)

    Honghong Liu

    2012-11-01

    Full Text Available The extraction parameters of intracellular polysaccharide (IPS from Hypsizigus marmoreus SK-01 mycelia were optimized, and the antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be: an ultrasonic treatment time of 618.07 s, an extraction temperature of 84.53 °C, a pH of 7.57, and an IPS yield of ca. 6.84%. The in vitro inhibition effects of IPS on hydroxyl, superoxide anion, and 1,1-diphenyl-2-picrylhydrazyl (DPPH radicals were 52.63 ± 3.19%, 68.21 ± 5.09%, and 63.43 ± 5.27%, respectively, and the EC50 values of IPS were 0.85 ± 0.05 g/L, 0.47 ± 0.03 g/L, and 0.62 ± 0.04 g/L, respectively. The activities of glutathione peroxidase (GSH-Px, superoxide dismutase (SOD, and catalase (CAT in mice blood were 2.52 ± 0.02 U/mL, 453.19 ± 38.43 U/mL, and 118.59 ± 9.64 U/mL, respectively. The results provide a reference for large-scale extraction of IPS by H. marmoreus SK-01 in industrial fermentation, suggesting that IPS can be used as a potential antioxidant that enhances adaptive immune responses.

  4. FEM analysis of hot-pressing process of aspheric glass P-SK57 lens

    Science.gov (United States)

    Zhu, Yongjian; Na, Jingxin; Wang, Yu; Li, Anhu; Zhi, Yanan; Fan, Yufeng

    2013-09-01

    Lens molding has become the promising technique to conduct mass produce of aspheric glass lens. It overcomes some disadvantages of traditional grinding or turning methods, such as high cost, low efficiency, unstable accuracy, and so on. Up to now, the lens molding process has been looked on as one of the reliable methods in fabrication of aspheric glass lens. However, in real production, one has found that it's hard to control the molding parameters, e.g. molding temperature, molding period, molding speed and pressing pressure, etc. Therefore it's necessary to develop the specific molding processes for a certain glass material. In this paper, SCHOTT P-SK57 is adopted to carry out the lens molding analysis in order to achieve the relative processing parameters. The molding cases are analyzed based on three different temperatures of 510°C, 520°C, and 530°C, higher than transition point 493°C of P-SK57. Through continuous heating and pressing simulation, the results show that the best pressing temperature could be about 530°C, at which the residual stress is only 5.22MPa (with the molding speed of 0.1mm/s).

  5. SK channel modulation rescues striatal plasticity and control over habit in cannabinoid tolerance.

    Science.gov (United States)

    Nazzaro, Cristiano; Greco, Barbara; Cerovic, Milica; Baxter, Paul; Rubino, Tiziana; Trusel, Massimo; Parolaro, Daniela; Tkatch, Tatiana; Benfenati, Fabio; Pedarzani, Paola; Tonini, Raffaella

    2012-01-08

    Endocannabinoids (eCBs) regulate neuronal activity in the dorso-lateral striatum (DLS), a brain region that is involved in habitual behaviors. How synaptic eCB signaling contributes to habitual behaviors under physiological and pathological conditions remains unclear. Using a mouse model of cannabinoid tolerance, we found that persistent activation of the eCB pathway impaired eCB-mediated long-term depression (LTD) and synaptic depotentiation in the DLS. The loss of eCB LTD, occurring preferentially at cortical connections to striatopallidal neurons, was associated with a shift in behavioral control from goal-directed action to habitual responding. eCB LTD and behavioral alterations were rescued by in vivo modulation of small-conductance calcium activated potassium channel (SK channel) activity in the DLS, which potentiates eCB signaling. Our results reveal a direct relationship between drug tolerance and changes in control of instrumental performance by establishing a central role for eCB LTD in habit expression. In addition, SK channels emerge as molecular targets to fine tune the eCB pathway under pathological conditions.

  6. Progesterone and estradiol enhance lipid mediated transfection of Sk-Br-3 mammalian cancer cells.

    Science.gov (United States)

    Köster, Frank; Felberbaum, Ricardo; Finas, Dominique; Wünsch, Kurt; Schulz, Christiane; Diedrich, Klaus; Hauser, Charlotte

    2002-06-01

    Cyclodextrin encapsulated beta-estradiol and progesterone were used for enhancement of gene delivery using the breast cancer cell line Sk-Br-3. A non-toxic concentration of cyclodextrin encapsulated sex steroids (50 microM) added to lipid or liposomal transfection led to a 12-fold increase of reporter gene expression (luciferase) with progesterone and an 8-fold increase with estradiol using Lipofectamine Plus mediated transfection. Using the lipid formulation Fugene-6 the results were a 5.5-fold and a 4.5-fold increase respectively. This enhancement could only be observed if the sex steroids were added to the cells before application of the DNA-Fugene complex supporting the evidence that intracellular processes are responsible for the activity of the steroids. The strong differences between progesterone and estradiol in modifying Lipofectamine Plus transfection in Sk-Br-3 cells may to be explained by differences in the distribution of these receptors in the cellular compartments. These results seem to add evidence on the possibility of using sex steroids to increase the efficiency of non-viral vectors for transfection, and may ultimately prove to be relevant to gene therapy in the treatment of breast cancer as well as other solid tumors.

  7. Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells.

    Science.gov (United States)

    Innajak, Sukanda; Mahabusrakum, Wilawan; Watanapokasin, Ramida

    2016-05-01

    Goniothalamin, a plant bioactive styrly-lactone, possesses many biological activities. In the present study, the anticancer effect of goniothalamin on human breast cancer cell line SK-BR-3 was investigated. The results showed that goniothalamin induced nuclear condensation, DNA fragmentation, apoptotic bodies and mitochondrial dysfunction as determined by JC-1 staining. Goniothalamin also increased the Bax/Bcl-2 ratio and expression of cleaved caspase-7, cleaved caspase-9 and cleaved PARP, but decreased Bcl-2 expression. In addition, goniothalamin induced apoptosis via p-JNK1/2 and p-p38 upregulation and inhibited cell survival via p-ERK1/2 and p-Akt downregulation. Notably, goniothalamin induced autophagy through upregulation of Atg7, Atg12-Atg5 conjugation and LC3II. The increased p-p38 and p-JNK1/2 and decreased p-Akt may lead to autophagy induction. Therefore, goniothalamin promoted apoptosis associated with autophagy induction in SK-BR-3 cells through p-p38 and p-JNK1/2 upregulation and p-Akt downregulation. The present study indicated that goniothalamin may be further used as a potential therapeutic candidate or may offer an alternative treatment for breast cancer.

  8. Resveratrol inhibits prostaglandin formation in IL-1β-stimulated SK-N-SH neuronal cells

    Directory of Open Access Journals (Sweden)

    Candelario-Jalil Eduardo

    2009-09-01

    Full Text Available Abstract Resveratrol, a polyphenol present in grapes and red wine, has been studied due to its vast pharmacological activity. It has been demonstrated that resveratrol inhibits production of inflammatory mediators in different in vitro and in vivo models. Our group recently demonstrated that resveratrol reduced the production of prostaglandin (PG E2 and 8-isoprostane in rat activated microglia. In a microglial-neuronal coculture, resveratrol reduced neuronal death induced by activated microglia. However, less is known about its direct roles in neurons. In the present study, we investigated the effects of resveratrol on interleukin (IL-1β stimulated SK-N-SH cells. Resveratrol (0.1-5 μM did not reduce the expression of cyclooxygenase (COX-2 and microsomal PGE2 synthase-1 (mPGES-1, although it drastically reduced PGE2 and PGD2 content in IL-1β-stimulated SK-N-SH cells. This effect was due, in part, to a reduction in COX enzymatic activity, mainly COX-2, at lower doses of resveratrol. The production of 8-iso-PGF2α, a marker of cellular free radical generation, was significantly reduced by resveratrol. The present work provides evidence that resveratrol reduces the formation of prostaglandins in neuroblastoma cells by reducing the enzymatic activity of inducible enzymes, such as COX-2, and not the transcription of the PG synthases, as demonstrated elsewhere.

  9. Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure.

    Science.gov (United States)

    Lim, Jia Chun; Thevarajoo, Suganthi; Selvaratnam, Chitra; Goh, Kian Mau; Shamsir, Mohd Shahir; Ibrahim, Zaharah; Chong, Chun Shiong

    2017-02-01

    Anoxybacillus sp. SK 3-4 is a Gram-positive, rod-shaped bacterium and a member of family Bacillaceae. We had previously reported that the strain is an aluminum resistant thermophilic bacterium. This is the first report to provide a detailed analysis of the global transcriptional response of Anoxybacillus when the cells were exposed to 600 mg L(-1) of aluminum. The transcriptome was sequenced using Illumina MiSeq sequencer. Total of 708 genes were differentially expressed (fold change >2.00) with 316 genes were up-regulated while 347 genes were down-regulated, in comparing to control with no aluminum added in the culture. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the majority of genes encoding for cell metabolism such as glycolysis, sulfur metabolism, cysteine and methionine metabolism were up-regulated; while most of the gene associated with tricarboxylic acid cycle (TCA cycle) and valine, leucine and isoleucine metabolism were down-regulated. In addition, a significant number of the genes encoding ABC transporters, metal ions transporters, and some stress response proteins were also differentially expressed following aluminum exposure. The findings provide further insight and help us to understand on the resistance of Anoxybacillus sp. SK 3-4 toward aluminium. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Differential Cytokine Changes in Patients with Myasthenia Gravis with Antibodies against AChR and MuSK.

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    Vuslat Yilmaz

    Full Text Available Neuromuscular transmission failure in myasthenia gravis (MG is most commonly elicited by autoantibodies (ab to the acetylcholine receptor or the muscle-specific kinase, constituting AChR-MG and MuSK-MG. It is controversial whether these MG subtypes arise through different T helper (Th 1, Th2 or Th17 polarized immune reactions and how these reactions are blunted by immunosuppression. To address these questions, plasma levels of cytokines related to various Th subtypes were determined in patients with AChR-MG, MuSK-MG and healthy controls (CON. Peripheral blood mononuclear cells (PBMC were activated in vitro by anti-CD3, and cytokines were quantified in supernatants. In purified blood CD4+ T cells, RNA of various cytokines, Th subtype specific transcription factors and the co-stimulatory molecule, CD40L, were quantified by qRT-PCR. Plasma levels of Th1, Th2 and Th17 related cytokines were overall not significantly different between MG subtypes and CON. By contrast, in vitro stimulated PBMC from MuSK-MG but not AChR-MG patients showed significantly increased secretion of the Th1, Th17 and T follicular helper cell related cytokines, IFN-γ, IL-17A and IL-21. Stimulated expression of IL-4, IL-6, IL-10 and IL-13 was not significantly different. At the RNA level, expression of CD40L by CD4+ T cells was reduced in both AChR-MG and MuSK-MG patients while expression of Th subset related cytokines and transcription factors were normal. Immunosuppression treatment had two effects: First, it reduced levels of IL12p40 in the plasma of AChR-MG and MuSK-MG patients, leaving other cytokine levels unchanged; second, it reduced spontaneous secretion of IFN-γ and increased secretion of IL-6 and IL-10 by cultured PBMC from AChR-MG, but not MuSK-MG patients. We conclude that Th1 and Th17 immune reactions play a role in MuSK-MG. Immunosuppression attenuates the Th1 response in AChR-MG and MuSK-MG, but otherwise modulates immune responses in AChR-MG and MuSK

  11. [Maria Skłodowska-Curie--her chemistry at the centenary of the second Nobel Prize].

    Science.gov (United States)

    Zagórski, Zbigniew Paweł; Kornacka, Ewa Maria

    2012-01-01

    The article presents from the perspective of one hundred years the work of Maria Curie-Skłodowska, which in many cases was ahead of the state of knowledge of the time. It opened new horizons and for this reason we made many digressions. The fact of awarding her the Nobel Prize twice is a sensation enough to present the values of careful activity of the Nobel Prize Committee that emphasizes the importance of Maria's achievements. A significant element of Maria Skłodowska-Curie's achievements was still mysterious character of the radiation in her time, and only chemical approach made it possible to organise the phenomena and explain the origin of the radiation. The essence of the research was an arduous separation of components following the track of growing radiation of successive fractions of preparations. This research was a start of the technology of educement of dispersed elements in great mass of materials. We underline the paramount role of the chemical research Maria Skłodowska conducted while still in Warsaw in the laboratories of the Museum of Industry and Agriculture under the guidance of an excellent chemist Józef Jerzy Boguski. Her research in Paris was the origin of the semi-commercial scale in chemistry and setting aside a special shed outside the university building was the beginning of the institutes that now function beyond universities and are key element of scientific and technical progress. Technology of splitting developed by Maria Skłodowska-Curie was applied also by other radiochemists, e.g. By Otto Hahn. Lively movement in radiochemistry of her lifetime resulted in Maria's disputes with e.g. German chemist Marckwald, who questioned the originality of polonium. The scientific disputes like this one Maria won triumphantly although in several others she had to accept opponents' argument, as in the case of radon. Her experiments were planned with utmost rationality as it was with the rejection of the hypothesis saying that radioactivity was

  12. A novel role for MuSK and non-canonical Wnt signaling during segmental neural crest cell migration.

    Science.gov (United States)

    Banerjee, Santanu; Gordon, Laura; Donn, Thomas M; Berti, Caterina; Moens, Cecilia B; Burden, Steven J; Granato, Michael

    2011-08-01

    Trunk neural crest cells delaminate from the dorsal neural tube as an uninterrupted sheet; however, they convert into segmentally organized streams before migrating through the somitic territory. These neural crest cell streams join the segmental trajectories of pathfinding spinal motor axons, suggesting that interactions between these two cell types might be important for neural crest cell migration. Here, we show that in the zebrafish embryo migration of both neural crest cells and motor axons is temporally synchronized and spatially restricted to the center of the somite, but that motor axons are dispensable for segmental neural crest cell migration. Instead, we find that muscle-specific receptor kinase (MuSK) and its putative ligand Wnt11r are crucial for restricting neural crest cell migration to the center of each somite. Moreover, we find that blocking planar cell polarity (PCP) signaling in somitic muscle cells also results in non-segmental neural crest cell migration. Using an F-actin biosensor we show that in the absence of MuSK neural crest cells fail to retract non-productive leading edges, resulting in non-segmental migration. Finally, we show that MuSK knockout mice display similar neural crest cell migration defects, suggesting a novel, evolutionarily conserved role for MuSK in neural crest migration. We propose that a Wnt11r-MuSK dependent, PCP-like pathway restricts neural crest cells to their segmental path.

  13. Comparison of visual, refractive and topographic keratometry outcomes of intacs and intacs SK in mild to moderate keratoconus eyes

    Directory of Open Access Journals (Sweden)

    Abdulrahman Al Muammar

    2015-01-01

    Full Text Available Purpose: The purpose was to evaluate and compare the visual and refractive outcomes, topographic keratometry (K and complications of Intacs and Intacs SK for mild to moderate keratoconus. Methods: In this retrospective study, all mild to moderate keratoconus eyes that underwent implantation of Intacs (Intacs group or Intacs SK (Intacs SK group with minimum follow-up of 12 months were included. Preoperative and postoperative uncorrected distance visual acuity (UDVA, corrected distance visual acuity (CDVA, manifest refraction, manifest cylinder, spherical equivalent (SE, minimum topographic keratometry, maximum topographic keratometry, and average topographic keratometry were compared in both groups. Results: There were 16 eyes in the Intacs group and 18 eyes in the Intacs SK group. Preoperatively, both groups were comparable for most parameters except gender and minimum K and average K. At 6 months postoperatively there were statistically significant improvements in UDVA, CDVA, manifest sphere, SE, minimum K, maximum K, and average K (P 0.05. The outcomes remained stable with no statistically significant differences between the 6 and 12 months visits. There were no complications in both groups. Conclusion: Both models of Intacs significantly improved vision and refractive outcomes, and topographic keratometry in cases of mild to moderate keratoconus. Intacs SK provided better (not statistically significant results.

  14. „Gott samstarf heimilis og skóla skilar sér alltaf“ : einkenni farsæls samstarfs heimilis og skóla á yngsta stigi grunnskóla

    OpenAIRE

    Lára Ágústa Hjartardóttir 1988

    2014-01-01

    Verkefnið snýst um samstarf heimilis og skóla á yngsta stigi grunnskóla. Tilgangurinn með rannsókninni var að varpa ljósi á hvað einkennir farsælt samstarf á yngsta stigi grunnskólans, hver ber ábyrgð og hverjar eru skyldur hvers aðila þegar kemur að samstarfinu. Markmiðið var að öðlast öryggi og styrk í samskiptum við heimilin og kynnast fjölbreyttum leiðum til að hvetja foreldra til þátttöku í skólastarfi barna sinna. Auk þess var skoðað hvernig samstarf heimilis og skóla hefur þróast á und...

  15. 10-(6'-Plastoquinonyl)decyltriphenylphosphonium (SkQ1) Does Not Increase the Level of Cytochromes P450 in Rat Liver and Human Hepatocyte Cell Culture.

    Science.gov (United States)

    Myasoedova, K N; Silachev, D N; Petrov, A D

    2016-12-01

    Mitochondria-targeted antioxidant SkQ1 did not increase the content of cytochromes P450 in livers of rats that were given SkQ1 in drinking water for 5 days in a dose (2.5 µmol per kg body weight) that exceeded 10 times the SkQ1 therapeutic dose. SkQ1 did not affect the levels of cytochrome P450 forms CYP1A2, CYP2B6, and CYP3A4 in monolayer cultures of freshly isolated human hepatocytes, while specific inducers of these forms (omeprazole, phenobarbital, and rifampicin, respectively) significantly increased expression of the cytochromes P450 under the same conditions. We conclude that therapeutic doses of SkQ1 do not induce cytochromes P450 in liver, and the absence of the inducing effect cannot be explained by poor availability of hepatocytes to SkQ1 in vivo.

  16. El retablo mayor de la parroquial de Vallecas (Madrid : una arquitectura desaparecida de Pedro y Francisco de la Torre (1672

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    Antonio José Díaz Fernández

    2004-01-01

    Full Text Available Como obra inédita dentro del catálogo de realizaciones del arquitecto y ensamblador de retablos Pedro de la Torre (1596-1677, uno de los principales artistas conformadores del arte barroco en el Madrid del siglo XVII, se ha de datar en 1672 el desaparecido retablo mayor de la iglesia parroquial de S. Pedro de Vallecas (Madrid, conocido hoy por fotografía, en el que intervienen otros artistas relevantes que aquí se documentan.As an unknown work belonging to the catalogue of performances of the architect and joiner of «retablos» Pedro de la Torre (1596-1677, one of the main creators of the baroque art at the Madrid of the XVIIth century, it’s possible to date in 1672 the missing main altarpiece of the S. Pedro’s church of Vallecas (Madrid, just known by photograph nowadays, where other important artists worked and are documented here.

  17. Comparing the role of absolute sea-level rise and vertical tectonic motions in coastal flooding, Torres Islands (Vanuatu).

    Science.gov (United States)

    Ballu, Valérie; Bouin, Marie-Noëlle; Siméoni, Patricia; Crawford, Wayne C; Calmant, Stephane; Boré, Jean-Michel; Kanas, Tony; Pelletier, Bernard

    2011-08-09

    Since the late 1990s, rising sea levels around the Torres Islands (north Vanuatu, southwest Pacific) have caused strong local and international concern. In 2002-2004, a village was displaced due to increasing sea incursions, and in 2005 a United Nations Environment Programme press release referred to the displaced village as perhaps the world's first climate change "refugees." We show here that vertical motions of the Torres Islands themselves dominate the apparent sea-level rise observed on the islands. From 1997 to 2009, the absolute sea level rose by 150 + /-20 mm. But GPS data reveal that the islands subsided by 117 + /-30 mm over the same time period, almost doubling the apparent gradual sea-level rise. Moreover, large earthquakes that occurred just before and after this period caused several hundreds of mm of sudden vertical motion, generating larger apparent sea-level changes than those observed during the entire intervening period. Our results show that vertical ground motions must be accounted for when evaluating sea-level change hazards in active tectonic regions. These data are needed to help communities and governments understand environmental changes and make the best decisions for their future.

  18. Comparing the role of absolute sea-level rise and vertical tectonic motions in coastal flooding, Torres Islands (Vanuatu)

    Science.gov (United States)

    Ballu, Valérie; Bouin, Marie-Noëlle; Siméoni, Patricia; Crawford, Wayne C.; Calmant, Stephane; Boré, Jean-Michel; Kanas, Tony; Pelletier, Bernard

    2011-08-01

    Since the late 1990s, rising sea levels around the Torres Islands (north Vanuatu, southwest Pacific) have caused strong local and international concern. In 2002-2004, a village was displaced due to increasing sea incursions, and in 2005 a United Nations Environment Programme press release referred to the displaced village as perhaps the world's first climate change "refugees." We show here that vertical motions of the Torres Islands themselves dominate the apparent sea-level rise observed on the islands. From 1997 to 2009, the absolute sea level rose by 150 + /-20 mm. But GPS data reveal that the islands subsided by 117 + /-30 mm over the same time period, almost doubling the apparent gradual sea-level rise. Moreover, large earthquakes that occurred just before and after this period caused several hundreds of mm of sudden vertical motion, generating larger apparent sea-level changes than those observed during the entire intervening period. Our results show that vertical ground motions must be accounted for when evaluating sea-level change hazards in active tectonic regions. These data are needed to help communities and governments understand environmental changes and make the best decisions for their future.

  19. The Gulf of Carpentaria heated Torres Strait and the Northern Great Barrier Reef during the 2016 mass coral bleaching event

    Science.gov (United States)

    Wolanski, E.; Andutta, F.; Deleersnijder, E.; Li, Y.; Thomas, C. J.

    2017-07-01

    The 2015/16 ENSO event increased the temperature of waters surrounding northeast Australia to above 30 °C, with large patches of water reaching 32 °C, for over two months, which led to severe bleaching of corals of the Northern Great Barrier Reef (NGBR). This study provides evidence gained from remote-sensing data, oceanographic data and oceanographic modeling, that three factors caused this excessive heating, namely: 1) the shutdown of the North Queensland Coastal Current, which would otherwise have flushed and cooled the Northern Coral Sea and the NGBR through tidal mixing 2) the advection of warm (>30 °C) water from the Gulf of Carpentaria eastward through Torres Strait and then southward over the NGBR continental shelf, and 3) presumably local solar heating. The eastward flux of this warm water through Torres Strait was driven by a mean sea level difference on either side of the strait that in turn was controlled by the wind, which also generated the southward advection of this warm water onto the NGBR shelf. On the NGBR shelf, the residence time of this warm water was longer inshore than offshore, and this may explain the observed cross-shelf gradient of coral bleaching intensity. The fate of the Great Barrier Reef is thus controlled by the oceanography of surrounding seas.

  20. Escolha de locais para instalação de torres de detecção de incêndio com auxílio do SIG

    Directory of Open Access Journals (Sweden)

    Nogueira Gilciano Saraiva

    2002-01-01

    Full Text Available O objetivo deste trabalho foi realizar um teste metodológico para avaliar as potencialidades de um sistema de informações geográficas e suas rotinas no planejamento de distribuição de torres de incêndio e na determinação do número ótimo de torres para visualização de uma área de estudo. A área estudada foi de 441,81 ha, em Belo Oriente-MG, compreendendo povoamentos de eucalipto, áreas de preservação permanente e reserva legal. Foram utilizados os temas vetoriais de uso do solo, recursos hídricos, malha viária e elevação. O processamento dos dados e das imagens e a produção dos mapas temáticos foram feitos no ArcView versão 3.1. Produziu-se um mapa de relevo interpolado e criou-se um tema de pontos, indicando os locais de instalação das torres. Foram escolhidos, inicialmente, oito pontos com base na proximidade de estradas e nos pontos mais elevados do terreno. A altura de cada torre foi fixada em 30 m. Utilizando a função Visibility do ArcView, pôde-se determinar a visibilidade da área a partir de cada ponto. A torre 1 apresentou a maior porcentagem de visibilidade (66,1%. Como a área estudada era pequena, decidiu-se pela instalação somente desta torre. Concluiu-se que a metodologia utilizada pode ser aplicada de maneira eficiente na determinação do número de torres necessárias e na confirmação ou não de locais previamente determinados para instalação das torres. Assim, com a utilização do sistema de informações geográficas aumenta-se a eficiência do sistema de vigilância e minimizam-se os custos com a instalação de torres.

  1. Różnice indywidualne w skłonności do kupowania impulsywnego. Konstrukcja i walidacja skali pomiarowej

    OpenAIRE

    Gąsiorowska, Agata

    2013-01-01

    Dotychczasowe badania nad zakupami impulsywnymi sugerują istnienie indy- widualnych różnic w zakresie skłonności do takiego sposobu kupowania. Niniejszy artykuł prezentuje wyniki badań, których celem była konstrukcja i weryfikacja wielowymiarowej skali do pomiaru skłonności do kupowania impulsywnego (SKI). Skala ta składa się z pięciu podwymiarów, reprezentujących emocje zwią- zane z zakupami, poczucie nacisku podczas zakupów, przyjemność z nich czerpaną, odczuwanie żalu pozakupowego i brak r...

  2. Overexpression of YB1 C-terminal domain inhibits proliferation, angiogenesis and tumorigenicity in a SK-BR-3 breast cancer xenograft mouse model.

    Science.gov (United States)

    Shi, Jian-Hong; Cui, Nai-Peng; Wang, Shuo; Zhao, Ming-Zhi; Wang, Bing; Wang, Ya-Nan; Chen, Bao-Ping

    2016-01-01

    Y-box-binding protein 1 (YB1) is a multifunctional transcription factor with vital roles in proliferation, differentiation and apoptosis. In this study, we have examined the role of its C-terminal domain (YB1 CTD) in proliferation, angiogenesis and tumorigenicity in breast cancer. Breast cancer cell line SK-BR-3 was infected with GFP-tagged YB1 CTD adenovirus expression vector. An 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) proliferation assay showed that YB1 CTD decreased SK-BR-3 cell proliferation, and down-regulated cyclin B1 and up-regulated p21 levels in SK-BR-3 cells. YB1 CTD overexpression changed the cytoskeletal organization and slightly inhibited the migration of SK-BR-3 cells. YB1 CTD also inhibited secreted VEGF expression in SK-BR-3 cells, which decreased SK-BR-3-induced EA.hy926 endothelial cell angiogenesis in vitro. YB1 CTD overexpression attenuated the ability of SK-BR-3 cells to form tumours in nude mice, and decreased in vivo VEGF levels and angiogenesis in the xenografts in SK-BR-3 tumour-bearing mice. Taken together, our findings demonstrate the vital role of YB1 CTD overexpression in inhibiting proliferation, angiogenesis and tumorigenicity of breast cancer cell line SK-BR-3.

  3. Inhibition of SK4 Potassium Channels Suppresses Cell Proliferation, Migration and the Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.

    Science.gov (United States)

    Zhang, Panshi; Yang, Xiaowei; Yin, Qian; Yi, Jilin; Shen, Wenzhuang; Zhao, Lu; Zhu, Zhi; Liu, Jinwen

    2016-01-01

    Treatments for triple-negative breast cancer (TNBC) are limited; intermediate-conductance calcium-activated potassium (SK4) channels are closely involved in tumor progression, but little is known about these channels in TNBC. We aimed to investigate whether SK4 channels affect TNBC. First, by immunohistochemistry (IHC) and western blotting (WB), increased SK4 protein expression in breast tumor tissues was detected relative to that in non-tumor breast tissues, but there was no apparent expression difference between various subtypes of breast cancer (p>0.05). Next, functional SK4 channels were detected in the TNBC cell line MDA-MB-231 using WB, real-time PCR, immunofluorescence and patch-clamp recording. By employing SK4 specific siRNAs and blockers, including TRAM-34 and clotrimazole, in combination with an MTT assay, a colony-formation assay, flow cytometry and a cell motility assay, we found that the suppression of SK4 channels significantly inhibited cell proliferation and migration and promoted apoptosis in MDA-MB-231 cells (pMDA-MB-231 cells to undergo the epithelial-mesenchymal transition (EMT) and to show increased SK4 mRNA expression. In addition, the down-regulation of SK4 expression inhibited the EMT markers Vimentin and Snail1. Collectively, our findings suggest that SK4 channels are expressed in TNBC and are involved in the proliferation, apoptosis, migration and EMT processes of TNBC cells.

  4. A clinical scoring system to identify patients with sebaceous neoplasms at risk for the Muir-Torre variant of Lynch syndrome.

    Science.gov (United States)

    Roberts, Maegan E; Riegert-Johnson, Douglas L; Thomas, Brittany C; Rumilla, Kandelaria M; Thomas, Colleen S; Heckman, Michael G; Purcell, Jennifer U; Hanson, Nancy B; Leppig, Kathleen A; Lim, Justin; Cappel, Mark A

    2014-09-01

    The Muir-Torre syndrome variant of Lynch syndrome is characterized by the presence of sebaceous neoplasms (adenoma, epithelioma/sebaceoma, carcinoma) and Lynch syndrome-associated cancers (colon, endometrial, and others). Several clinical scoring systems have been developed to identify patients with colon cancer at high risk of Lynch syndrome. However, no such system has been described for patients presenting with sebaceous neoplasms. Based on logistic regression analysis, a scoring system was developed for patients with sebaceous neoplasm to identify those with the highest likelihood of having Muir-Torre syndrome. The final version of the scoring system included variables such as age at presentation of initial sebaceous neoplasm, total number of sebaceous neoplasms, personal history of a Lynch-related cancer, and family history of Lynch-related cancers. Patients with a score of 3 or more were more likely to have Muir-Torre syndrome (28 of 29 patients), those with a score of 2 had intermediate likelihood (12 of 20 patients), and no patient with a score of 0 or 1 was diagnosed with Muir-Torre syndrome. The Mayo Muir-Torre syndrome risk scoring system appears to identify whether patients who present with sebaceous neoplasms are in need of further Lynch syndrome evaluation using easily ascertained clinical information. Abnormal mismatch repair gene immunohistochemistry of a sebaceous neoplasm is a poor predictor in regard to diagnosing Lynch syndrome.

  5. Complete Sequences of Four Plasmids of Lactococcus lactis subsp. cremoris SK11 Reveal Extensive Adaptation to the Dairy Environment†

    Science.gov (United States)

    Siezen, Roland J.; Renckens, Bernadet; van Swam, Iris; Peters, Sander; van Kranenburg, Richard; Kleerebezem, Michiel; de Vos, Willem M.

    2005-01-01

    Lactococcus lactis strains are known to carry plasmids encoding industrially important traits. L. lactis subsp. cremoris SK11 is widely used by the dairy industry in cheese making. Its complete plasmid complement was sequenced and found to contain the plasmids pSK11A (10,372 bp), pSK11B (13,332 bp), pSK11L (47,165 bp), and pSK11P (75,814 bp). Six highly homologous repB-containing replicons were found, all belonging to the family of lactococcal theta-type replicons. Twenty-three complete insertion sequence elements segment the plasmids into numerous modules, many of which can be identified as functional units or containing functionally related genes. Plasmid-encoded functions previously known to reside on L. lactis SK11 plasmids were now mapped in detail, e.g., lactose utilization (lacR-lacABCDFEGX), the proteolytic system (prtM-prtP, pepO, pepF), and the oligopeptide permease system (oppDFBCA). Newly identified plasmid-encoded functions could facilitate the uptake of various cations, while the pabA and pabB genes could be essential for folate biosynthesis. A competitive advantage could be obtained by using the putative flavin adenine dinucleotide-dependent d-lactate dehydrogenase and oxalate:formate antiporter for enhanced ATP synthesis, while the activity of the predicted α-acetolactate decarboxylase may contribute to the formation of an additional electron sink. Various stress response proteins are plasmid encoded, which could enhance strain robustness. A substantial number of these “adaptation” genes have not been described before on L. lactis plasmids. Moreover, several genes were identified for the first time in L. lactis, possibly reflecting horizontal gene transfer. PMID:16332824

  6. The Making of the Gdańsk Metropolitan Region. Local Discourses of Identities, Powers, and Hopes

    Directory of Open Access Journals (Sweden)

    Czepczyński Mariusz

    2014-12-01

    Full Text Available The process of metropolitanisation of the Gdańsk area is facilitated by public discourse involving local and regional politicians, media, and inhabitants. The discussion is based upon historical narrations, but also local ambitions, hopes and emotions, as well as infrastructural projects and investment attractiveness. Foucault suggests that modern power is a dispersed set of micro-practices, many of which operate through the normalising gaze of surveillance regimes. Gdańsk metropolitan cooperation, competition and encounters make the core of the paper; local and regional unifying initiatives and processes are accompanied by examples of separatism, identity conflicts, and political disagreements

  7. Genetic Pd, Pt, Au, Ag, and Rh mineralogy in Noril'sk sulfide ores

    Science.gov (United States)

    Spiridonov, E. M.; Kulagov, E. A.; Serova, A. A.; Kulikova, I. M.; Korotaeva, N. N.; Sereda, E. V.; Tushentsova, I. N.; Belyakov, S. N.; Zhukov, N. N.

    2015-09-01

    The undeformed ore-bearing intrusions of the Noril'sk ore field (NOF) cut through volcanic rocks of the Late Permian-Early Triassic trap association folded in brachysynclines. Due to the nonuniform load on the roof of intrusive bodies, most sulfide melts were squeezed, up to the tops of ore-bearing intrusions; readily fusible Ni-Fe-Cu sulfide melts were almost completely squeezed. In our opinion, not only one but two stages of mineralization developed at the Noril'sk deposits: (i) syntrap magmatic and (ii) epigenetic post-trap metamorphic-hydrothermal. All platinum-group minerals (PGM) and minerals of gold are metasomatic in the Noril'sk ores. They replaced sulfide solid solutions and exsolution structures. All types of PGM and Au minerals occur in the ores, varying in composition from pyrrhotite to chalcopyrite, talnakhite, mooihoekite, and rich in galena; they are localized in the inner and outer contact zones and differ only in the quantitative proportions of ore minerals. The aureoles of PGM and Au-Ag minerals are wider than the contours of sulfide bodies and coincide with halos of fluid impact on orebodies and adjacent host rocks. The pneumatolytic PGM and Au-Ag minerals are correlated in abundance with the dimensions of sulfide bodies. Their amounts are maximal in veins of late fusible ore composed of eutectic PbS ss and iss intergrowths, as well as at their contacts. The Pd and Pt contents in eutectic sulfide ores of NOF are the world's highest. In the process of noble-metal mineral formation, the fluids supply Pd, Pt, Au, As, Sb, Sn, Bi, and a part of Te, whereas Fe, Ni, Cu, Pb, Ag, Rh, a part of Te and Pd are leached from the replaced sulfide minerals. The pneumatolytic PGM of the early stage comprises Pd and Pt intermetallic compounds enriched in Au along with Pd-Pt-Fe-Ni-Cu-Sn-Pb(As) and (Pd,Pt,Au)(Sn,Sb,Bi,Te,As) solid solutions. Pneumatolytic PGM and Au minerals of the middle stage are products of solid-phase transformation and recrystallization of

  8. Parrot SK400摩托车专用蓝牙套装

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    专门做手机无线接口设备的Parrot公司,近期发表了这款Parrot SK400摩托车专用蓝牙免持套件。配有FM无线电播放器和无线手机遥控器。“骑士们”可以自由选择听音乐、广播或接电话。多声器的语音辨识系统,可以辨识联络人姓名并可自动接打电话。特别是Text To Speech功能,可以透过耳机,

  9. Convergence of clock processes in random environments and ageing in the p-spin SK model

    CERN Document Server

    Bovier, Anton

    2010-01-01

    We derive a general criterion for the convergence of clock processes in random dynamics in random environments that is applicable in cases when correlations are not negligible, extending recent results by Gayrard [15,16], based on general criterion for convergence of sums of dependent random variables due to Durrett and Resnick [13]. We demonstrate the power of this criterion by applying it to the case of random hopping time dynamics of the p-spin SK model. We prove that on a wide range of time scales, the clock process converges to a stable subordinator almost surely with respect to the environment. We also show that a time-time correlation function converges to the arcsine law for this subordinator, almost surely. This improves recent results of Ben Arous et al. [1] that obtained similar convergence result in law with respect to the random environment.

  10. LA TORRE ALMENARA DE SAN GARCÍA, ALGECIRAS (S. XVII-XVIII. RECUPERACIÓN VIRTUAL DE UNA ESTRUCTURA MILITAR GRACIAS A LA TECNOLOGÍA

    Directory of Open Access Journals (Sweden)

    Pablo Aparicio Resco

    2017-02-01

    Full Text Available La reconstrucción virtual de la torre almenara de San García se enmarca dentro de las actuaciones para crear en Gibraltar el Centro de Interpretación de la Re- serva de la Biosfera Intercontinental del Mediterráneo “Estrecho de Gibraltar”, un proyecto llevado a cabo por la empresa Geasense. Actualmente sólo se conserva parte de la cimentación de esta torre defensiva por lo que la reconstrucción vir- tual de la misma tuvo que ser realizada en base a paralelos bien documentados a lo largo de la costa sur de nuestra Península. Este proyecto ha permitido mostrar cómo pudo ser en el pasado la torre, cuál fue su función y su valor patrimonial para las gentes de la Bahía de Algeciras.

  11. Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma

    Directory of Open Access Journals (Sweden)

    Ferreira Ricardo B

    2011-01-01

    Full Text Available Abstract Background RNA quality and quantity are important factors for ensuring the accuracy of gene expression analysis and other RNA-based downstream applications. Extraction of high quality nucleic acids is difficult from neuronal cells and brain tissues as they are particularly rich in lipids. In addition, most common RNA extraction methods are phenol-based, resulting in RNA that may be incompatible with downstream applications such as gene expression. Findings In this work, a comparative analysis of the RNA quality obtained from SK-N-MC cells was performed using six commonly used RNA isolation kits: two phenol-based kits and four non-phenol based kits. The non-phenol based kits tested AxyPrep Multisource Total RNA Miniprep, RNeasy® Mini, EasySpin and Ilustra RNAspin Mini RNA Isolation, all performed well and resulted in the isolation of high quality RNA, as evaluated by A260/A280. The RNA extracted with AxyPrep Multisource Total RNA Miniprep, RNeasy® Mini and EasySpin provided the highest RNA yields. In particular, the RNA isolated by AxyPrep Multisource Total RNA Miniprep Kit did not show any detectable genomic DNA contamination even without previous DNase treatment or after RNA direct PCR amplification using universal 18S primers. Conclusions The RNA extracted from SK-N-MC cells with AxyPrep Multisource Total RNA Miniprep Kit was superior with respect to the RNA quality and concentration. This kit does not use aggressive organic solvents and RNA free of genomic DNA was isolated without the need for DNase treatment.

  12. Human ortholog of a plant salicylic acid receptor found in SK-N-SH cell line.

    Science.gov (United States)

    Skubatz, Hanna; Howald, William N

    2013-12-01

    Our previous studies have described the purification and characterization of a novel plant NAD(P)-reductase like protein (RL) from the thermogenic appendix of the Sauromatum guttatum inflorescence. RL is mainly located in cytoplasm of thermogenic plants and it can act like a bistable switch. It adopts a compact conformation during heat-production and a more expanded conformation when heat is not generated. Addition of salicylic acid, a natural thermogenic inducer, at picomolar concentration to a solution of purified RL induced a discontinuous volume phase transition in which the volume of RL in the oligomeric form expanded and shrunk repeatedly every 4-5 min. In the present study using ESI-MS analysis we have demonstrated the existence of RL in the human SK-N-SH cell line and in mouse brain tissue. The molecular mass of human RL is in the same range as of its plant counterpart, 34,140 ± 34 Da. The charge state distribution of the human RL is identical to its plant counterpart from the Sauromatum appendix during heat-production. Human RL was present in the compact state when it was purified from the SK-N-SH cell line When these cells were treated with salicylic acid (10 μM) a shift to a much more compact conformation was observed. It seems that the potential of RL to respond to salicylic acid was conserved. These results may reveal the existence of a thermoregulation system that is evolutionarily conserved and is operating by conformational changes. This discovery may also represent an opportunity for a better understanding of some of the diverse functions of salicylic acid and aspirin in plants and humans.

  13. Análisis estructural de la torre del homenaje de la Alhambra de Granada (España

    Directory of Open Access Journals (Sweden)

    Villegas, D.

    2014-12-01

    Full Text Available This paper aim, on one hand, to describe the structural response of “Homenaje Tower”, one of the towers of the Historical Complex of the Alhambra, in Granada (Spain and, on the other hand, to look over the different techniques used in the structural analysis of historical buildings, applying them to the tower to assess the scope of each of them. The tower is analyzed from three methods that count on different levels of complexity. First, an analysis using graphical method for limit analysis is carried out to assess the different safety coefficients from a stability point of view. Secondly, a finite element linear elastic analysis is applied in order to identify possible stress concentration zones, in particular zones with tensile stresses. Finally, a nonlinear static pushover analysis has been carried out over five different numerical models to assess the structural response to horizontal loads of perimeter walls and interior porticos.Este artículo pretende describir la respuesta estructural de la Torre del Homenaje, una de las torres del complejo de la Alhambra (Granada, y por otro lado revisar las distintas técnicas utilizadas en el análisis estructural de edificios históricos aplicándolas a dicho objeto para valorar el alcance de cada una de ellas. La torre se analiza mediante tres procedimientos con diferentes niveles de complejidad. En primer lugar, se utiliza el método de líneas de empuje para valorar los diferentes coeficientes de seguridad desde un punto de vista de la estabilidad. En segundo lugar, se lleva a cabo un análisis elástico y lineal aplicando el método de los elementos finitos para identificar posibles concentraciones de tensiones de tracción. Por último, se ha realizado un análisis estático no lineal tipo pushover sobre cinco modelos numéricos diferentes para valorar la respuesta estructural ante acciones horizontales de los muros perimetrales y de los pórticos interiores.

  14. Los usuarios y las webs de los archivos históricos nacionales: el caso del Arquivo Nacional da Torre do Tombo (Portugal Webs and national archives users: the case of Arquivo Nacional de Torre do Tombo (Portugal

    Directory of Open Access Journals (Sweden)

    Amalia Más Bleda

    2009-04-01

    Full Text Available El objetivo de este artículo es conocer cuál es la información o los servicios que se deberían incluir en el sitio web de un archivo, tomando como referencia la opinión de su público. Para ello analizamos las necesidades de 90 usuarios de un archivo concreto: el Arquivo Nacional da Torre do Tombo (Archivo Nacional de Portugal, obtenidas mediante la utilización de una entrevista personal. Se concluye que la mayoría de los usuarios desean encontrar en el sitio web información básica o práctica, como datos de contacto y condiciones de acceso y consulta, así como una descripción detallada de los fondos que custodia el archivo, el mayor número posible de documentos digitalizados, un buen sistema de búsqueda (catálogo y la posibilidad de hacer trámites en línea.Information is given of services that should be included in the website of an archive, taking as reference the opinion of its public. Therefore the needs of 90 users of this concrete archive, the Arquivo Nacional da Torre do Tombo (National Archive of Portugal are analyzed, which were obtained through a personal interview. Conclusions are that most users wish to find basic or practical information in the web, such as contact, access and consultation data; a detailed description of the archive's funds; the number of digitalized documents on-line; a good search system (catalog, and the possibility of doing transactions on-line.

  15. Antioxidant mechanism of mitochondria-targeted plastoquinone SkQ1 is suppressed in aglycemic HepG2 cells dependent on oxidative phosphorylation.

    Science.gov (United States)

    Ježek, Jan; Engstová, Hana; Ježek, Petr

    2017-09-01

    Previously suggested antioxidant mechanisms for mitochondria-targeted plastoquinone SkQ1 included: i) ion-pairing of cationic SkQ1(+) with free fatty acid anions resulting in uncoupling; ii) SkQ1H2 ability to interact with lipoperoxyl radical; iii) interference with electron flow at the inner ubiquinone (Q) binding site of Complex III (Qi), involving the reduction of SkQ1 to SkQ1H2 by ubiquinol. We elucidated SkQ1 antioxidant properties by confocal fluorescence semi-quantification of mitochondrial superoxide (Jm) and cytosolic H2O2 (Jc) release rates in HepG2 cells. Only in glycolytic cells, SkQ1 prevented the rotenone-induced enhancement of Jm and Jc but not basal releases without rotenone. The effect ceased in glutaminolytic aglycemic cells, in which the redox parameter NAD(P)H/FAD increased after rotenone in contrast to its decrease in glycolytic cells. Autofluorescence decay indicated decreased NADPH/NADH ratios with rotenone in both metabolic modes. SkQ1 did not increase cell respiration and diminished Jm established high by antimycin or myxothiazol but not by stigmatellin. The revealed SkQ1 antioxidant modes reflect its reduction to SkQ1H2 at Complex I IQ or Complex III Qi site. Both reductions diminish electron diversions to oxygen thus attenuating superoxide formation. Resulting SkQ1H2 oxidizes back to SkQ1at the second (flavin) Complex I site, previously indicated for MitoQ10. Regeneration proceeds only at lower NAD(P)H/FAD in glycolytic cells. In contrast, cyclic SkQ1 reduction/SkQ1H2 oxidation does not substantiate antioxidant activity in intact cells in the absence of oxidative stress (neither pro-oxidant activity, representing a great advantage). A targeted delivery to oxidative-stressed tissues is suggested for the effective antioxidant therapy based on SkQ1. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Effects of rhaponticin on proliferation of SK-BR-3 breast cancer cells%土大黄苷对乳腺癌细胞SK-BR-3增殖的影响

    Institute of Scientific and Technical Information of China (English)

    赵素容; 段海峰; 许尹; 卢兖伟; 蒋志文

    2010-01-01

    目的 观察土大黄苷对乳腺癌细胞SK-BR-3增殖的影响,并初步探讨其作用机制.方法 采用MTT法检测土大黄苷对SK-BR-3细胞的增殖抑制作用,倒置显微镜下观察细胞的形态学变化,Western印迹检测Akt及p-Akt蛋白的表达情况.结果 土大黄苷可抑制SK-BR-3细胞增殖,其作用具有浓度和时间依赖性,72 h的IC50为(181.3±11.4)μmol/L.200、400 μmol/L土大黄苷作用48 h后显示出明显的细胞毒作用,显微镜下可见细胞碎片增多、细胞密度减小.土大黄苷在100、200、400 μmol/L浓度时均可抑制p-Akt蛋白的表达.结论 土大黄苷可抑制SK-BR-3细胞的增殖,其机制可能与抑制p-Akt表达有关.

  17. Epidemiology, etiology, and motivation of alcohol misuse among Australian Aboriginal and Torres Strait Islanders of the Northern Territory: a descriptive review.

    Science.gov (United States)

    Ramamoorthi, Ramya; Jayaraj, Rama; Notaras, Leonard; Thomas, Mahiban

    2015-01-01

    The per capita alcohol consumption of the Northern Territory, Australia, is second highest in the world, estimated 15.1 liters of pure alcohol per year. Alcohol abuse is a major public health concern among Aboriginal and Torres Strait Islander people in Australia. The Aboriginal and Torres Strait Islanders in the Northern Territory consume approximately 16.9 liters of pure alcohol per year. This descriptive review is based on current published and grey literature in the context of high risk alcohol use, with a special focus on the epidemiological, etiological, and social factors, to predict alcohol misuse among the Australian Aboriginal and Torres Strait Islanders in Northern Territory. The methodology involved a descriptive search on PubMed, Northern Territory government reports, health databases, and Web sites with an emphasis on the etiology and epidemiology of high-risk alcohol consumption among the Australian Aboriginal and Torres Strait Islanders of the Northern Territory. This review has its own limitations because it does not rely on systematic review methodologies. However, it presents real data on the motives for binge drinking and alcohol-related violent assaults of this vulnerable population. Alcohol abuse and alcohol-related harms are considerably high among the rural and remote communities where additional research is needed. High-risk alcohol misuse within Australian Aboriginal and Torres Strait Islanders communities often leads to a series of physical and social consequences. This review highlights the need for culturally appropriate intervention approaches focusing on alcohol misuse among the Aboriginal and Torres Strait Islanders population of the Northern Territory.

  18. Effect of morphine on L-calcium channel of SK-N-SH human neuroblastoma cells%吗啡对人神经母细胞瘤SK-N-SH细胞L-Ca2+ 通道的作用

    Institute of Scientific and Technical Information of China (English)

    雷洪伊; 徐世元; 张庆国; 叶小平

    2008-01-01

    Objective To study the effect of morphine on L-calcium channels of SK-N-SH human neuroblastoma cells.Metllods SK-N-SH human neuroblastoma cells were cultured in vitro. Electrical current and conductance of calcium channels of SK-N-SH cells were measured using cell-attached patch clamp technique in the presence of morphine with different concentrations(0.10-8 mol/L,10-7 mol/L,10-6 mol/L and 10-5 mol/L)preteated with or without naloxone(10-5 mol/L).Results Electrical current of L-calcium channels of SK-N-SH human neuroblastoma cells was concentration-dependently depressed by morphine(P <0.01 at concentrations of 10-7,10-6 and 10-5 mol/L).Pretreatment with naloxone eliminated the morphine-induced depression on electrical current of calcium channels. Electric conductance of calcium channels did not change in the presence or absence of morohine. Conclusions Morphine inhibits electric current of L-type calcium channels of SK-N-SH human neuroblastoma cells in a manner of concentration dependence,probably via blockade of μ-opioid receptor;but does not influence electric conductence ot L-type calcium channels.%目的 观察吗啡对人神经母细胞瘤SK-N-SH细胞L-Ca2+通道的影响.方法 培养人神经母细胞瘤SK-N-SH细胞,应用细胞贴附式膜片钳技术记录在不同浓度吗啡(0、10-8 mol/L、10-7 mol/L、10-6 mol/L、10-5 mol/L)作用下SK-N-SH细胞L-Ca2+通道电流和电导变化.预先加入10-5 mol/L纳洛酮后,再依次加入上述不同浓度吗啡,记录此通道电流和电导.结果 10-7 mol/L、10-6 mol/L、10-5 mol/L吗啡可抑制SK-N-SH细胞L-型Ca2+通道电流,吗啡浓度为10-6mol/L、10-5 mol/L时抑制作用更明显(P<0.01),具有浓度依赖性;各浓度吗啡对L-Ca2+通道电导无影响;预先加入纳络酮,可阻断吗啡对该通道的抑制作用.结论 吗啡可抑制SK-N-SH细胞上L-Ca2+通道电流,对该通道电导无作用.纳络酮可翻转吗啡的作用,其抑制作用可能通过μ阿片受体产生.

  19. Translators and teachers of languages: grammars and vocabularies, Francisco de la Torre y Ocón (1728-1731

    Directory of Open Access Journals (Sweden)

    M.ª del Carmen CAZORLA VIVAS

    2013-03-01

    Full Text Available The 18th Century has not been studied in depth by experts on Historical Linguistics. However, this is a fruitful and important period in the history of dictionaries as far as lexicography is concerned. The present paper focuses on the lexicographer and translator Francisco de la Torre y Ocón (1660-1725 and his work El maestro de las dos lenguas. Diccionario español y francés en dos tomos. (1726-1728. The work by this renowned author will be analyzed within the framework of its historical and linguistic period (first half of the 18th Century. His approach to lexicography will be similarly analyzed with the aim of contributing to a better understanding of bilingual (French-Spanish lexicography.

  20. THE EFFECT OF CASEIN HYDROLYSATES ON TRANSGLUTAMINASE PRODUCTION BY STRETOVERTICILLUM SK-2%酪蛋白水解物对轮枝链霉菌SK-1产谷氨酰胺转胺酶的影响

    Institute of Scientific and Technical Information of China (English)

    常中义; 江波; 王璋

    2001-01-01

    酪蛋白经胰酶水解后,可以被轮枝链霉菌SK-1所利用.采用pH-STAT法得到了DH=3、DH=6、DH=9、DH=12等4个水解度的酪蛋白水解物.当酪蛋白水解物作氮源时,其水解度(即肽相对分子质量的大小)对轮枝链霉菌SK-1产谷氨酰胺转胺酶酶影响很大,其中以DH=3的酪蛋白水解物效果最好,而且肽的浓度对轮枝链霉菌SK-1产谷胺酰胺转胺酶也有一定的影响,其中以4%的浓度最为合适.%After being hydrolyzing,casein was utilized by Stretoverticillium SK-2.Casein hydrolyzated (DH=3,DH=6,DH=9,DH=12)wer e obtained wit h pancreatin.When the casein hydrolyzates were used as a nitrogen source,the der gree of hydrolysis(the molecular weight of casein peptide)had a significant infl uence on the production of transglutaminae by Stretoverticillium SK- 2 and the op timum value was DH=3,The production of transglutaminase also depend on the c oncentration of casein peptide and,with 4% addition of DH=3 casein peptide,t he production of transglutaminase reached 3.89 u/mL.

  1. 熊果酸对乳腺癌SK-BR-3细胞增殖和凋亡的影响%Effects of Ursolic Acid on Proliferation and Apoptosis of SK-BR-3 Cells

    Institute of Scientific and Technical Information of China (English)

    徐新伟; 郭玲玲; 顾振纶; 蒋小岗; 周文轩; 郭次仪

    2009-01-01

    目的 探讨熊果酸(UA)对ER(-)、Her-2过表达的人乳腺癌SK-BR-3细胞增殖和凋亡的影响.方法 利用噻唑蓝(MTT)比色法观察UA对SK-BR-3细胞增殖的影响.用Hoechst 33258荧光染色及电子显微镜观察凋亡细胞核形态变化.流式细胞仪检测UA对细胞周期的影响;DNA琼脂糖凝胶电泳观察DNA片段的断裂.结果 UA明显抑制SK-BR-3细胞的增殖,差异有统计学意义(PSK-BR-3细胞阻滞在G0/G1期,差异有统计学意义(均P<0.05).DNA琼脂糖凝胶电泳显示DNA大片段的断裂.结论 UA在体外对SK-BR-3细胞具有抗增殖和诱导凋亡的作用.

  2. Hacia la construcción del gracioso: Carnaval y metateatralidad en los pastores de Bartolomé Torres Naharro

    Directory of Open Access Journals (Sweden)

    Julio Vélez-Sainz

    2009-09-01

    Full Text Available Resumen: Es común en la crítica del teatro áureo destacar la función metaliteraria del personaje del gracioso. Éste funcionaría como un elemento casi ficticio dentro de la ficción de la comedia (la “ilusión escénica” y sus apartes dramáticos marcan el ritmo de ésta, lo que le serviría a los autores a ejercer una cierta crítica literaria a partir de estos personajes. El siguiente estudio plantea un acercamiento a los albores del teatro laico en la España pre-renacentista que desvela la metateatralidad de las figuras cómicas del teatro prelopista. Sostenemos que la autorreferencialidad del gracioso que tanto ha destacado la crítica es, en realidad, un rasgo heredado de sus antepasados teatrales: los pastores del pacense Bartolomé de Torres Naharro.Summary: Theater critics have insisted upon the metaliterary function of the gracioso stock-character in the Spanish Golden Age. This comic figure works as a quasi-fictitious element within the theatrical fiction (the “scenic illusion”, as some have termed and his dramatic comments and asides would serve to path the rhythm of the scenes. This character could also be used to insert comments of literary criticism The following study approaches dramatis personae of lay Renaissance theater that unveil the metatheatricality of the pre-Lopean comic figures. I thus assert that the self-referentiality of the gracioso is, in fact, a trait inherited from the gracioso´s predecessors, among them the comic shepherds of the Badajoz-born author Bartolomé de Torres Naharro.

  3. With good intentions: complexity in unsolicited informal support for Aboriginal and Torres Strait Islander peoples. A qualitative study

    Directory of Open Access Journals (Sweden)

    Aspin Clive

    2011-09-01

    Full Text Available Abstract Background Understanding people's social lived experiences of chronic illness is fundamental to improving health service delivery and health outcomes, particularly in relation to self-management activity. In explorations of social lived experiences this paper uncovers the ways in which Aboriginal and Torres Strait Islander people with chronic illness experience informal unsolicited support from peers and family members. Methods Nineteen Aboriginal and Torres Islander participants were interviewed in the Serious and Continuing Illness Policy and Practice Study (SCIPPS. Participants were people with Type 2 diabetes (N = 17, chronic obstructive pulmonary disease (N = 3 and/or chronic heart failure (N = 11 and family carers (N = 3. Participants were asked to describe their experience of having or caring for someone with chronic illness. Content and thematic analysis of in-depth semi-structured interviews was undertaken, assisted by QSR Nvivo8 software. Results Participants reported receiving several forms of unsolicited support, including encouragement, practical suggestions for managing, nagging, growling, and surveillance. Additionally, participants had engaged in 'yarning', creating a 'yarn' space, the function of which was distinguished as another important form of unsolicited support. The implications of recognising these various support forms are discussed in relation to responses to unsolicited support as well as the needs of family carers in providing effective informal support. Conclusions Certain locations of responsibility are anxiety producing. Family carers must be supported in appropriate education so that they can provide both solicited and unsolicited support in effective ways. Such educational support would have the added benefit of helping to reduce carer anxieties about caring roles and responsibilities. Mainstream health services would benefit from fostering environments that encourage informal interactions that

  4. El cine musical de Roberta Torre: un retrato postmoderno de la Sicilia de finales del siglo XX

    Directory of Open Access Journals (Sweden)

    Viñuela, Eduardo

    2012-12-01

    Full Text Available The renewal of the language of the musical at the turn of the century allowed for the development of new narratives in cinema through intertextualilty and parody. In Tano da morire (1997 and Sud side story (2000 Roberta Torre depicts a Sicily caught up in a process of transformation between tradition and modernity. Allusion to some of the stereotypes and clichés about southern Italy (the mafia, immigration, religion, popular music, etc. using a postmodern narrative offers us a fresh view of Sicily, far from the traditional platitudes.

    A finales del siglo XX el cine musical renovó su lenguaje, dando lugar a nuevas narrativas en las que la intertextualidad y la parodia permitían nuevas significaciones y múltiples lecturas. Roberta Torre se erige en una figura representativa del cine musical contemporáneo en Italia, y en sus películas Tano da morire (1997 y Sud side story (2000 nos presenta el retrato de una Sicilia en proceso de cambio y negociación entre tradición y modernidad. La alusión a los tópicos y a los estereotipos que configuran el imaginario de la Italia meridional (mafia, inmigración, religión, música popular, etc. dentro de una narrativa postmoderna ofrece una visión diferente que desmonta los clichés tradicionalmente asociados con Sicilia.

  5. [Mitochondria-targeted antioxidant SkQR1 selectively protects MDR-negative cells against ionizing radiation].

    Science.gov (United States)

    Fetisova, E K; Antoshina, M M; Cherepanynets, V D; Izumov, D S; Kireev, I I; Kireev, R I; Lyamzaev, K G; Riabchenko, N I; Chernyak, B V; Skulachev, V P

    2014-01-01

    Radioprotection appeared to be an important problem of today due to atom energetic development and utilization of radiation material in the industry, science and medicine. It has been shown that mitochondrial targeted antioxidant SkQR1 could attenuate radiation injury of human erythroleukemia K562 cells. Pretreatment with SkQR1 before irradiation decreased DNA double strand breaks formation, diminished the number of chromosomal aberrations and suppressed delayed ROS production. Prevention of oxidative stress and normalization of mitochondrial function by mitochondria-targeted antioxidants may be a potential therapeutic strategy not only against immediate consequences of radiation, but, either against its late consequences such as genomic instability. SkQR1 did not protect against radiation-induced damage the K562 subline with high level of multidrug resistance (MDR) due to SkQR1 extrusion with Pgp 170 MDR pump. We suggest that mitochondria-targeted antioxidants might be used for selective protection of normal cells against radiation-induced damage without interference with radiotherapy of MDR-positive tumors.

  6. Transcription profiling of interactions between Lactococcus lactis subsp. cremoris SK11 and Lactobacillus paracasei ATCC 334 during Cheddar cheese simulation.

    Science.gov (United States)

    Desfossés-Foucault, Émilie; LaPointe, Gisèle; Roy, Denis

    2014-05-16

    The starter cultures (Lactococcus sp.) and non-starter lactic acid bacteria (mostly Lactobacillus spp.) are essential to flavor development of Cheddar cheese. The aim of this study was to elucidate the transcriptional interaction between Lactococcus lactis subsp. cremoris SK11 and Lactobacillus paracasei ATCC 334 in mixed cultures during simulated Cheddar cheese manufacture (Pearce activity test) and ripening (slurry). Reverse transcription quantitative PCR (RT-qPCR) was used to quantify the expression of 34 genes common to both bacteria and for eight genes specific to either L. lactis subsp. cremoris SK11 or L. paracasei ATCC 334. The multifactorial analysis (MFA) performed on fold change results for each gene revealed that the genes linked to stress, protein and peptide degradation as well as carbohydrate metabolism of L. paracasei ATCC 334 were especially overexpressed in mixed culture with L. lactis subsp. cremoris SK11 during the ripening simulation. For L. lactis subsp. cremoris SK11, genes coding for amino acid metabolism were more expressed during the cheese manufacture simulation, especially in single culture. These results show how complementary functions of starter and NSLAB contribute to activities useful for flavor development.

  7. Active uptake and extravesicular storage of m-iodobenzylguanidine in human neuroblastoma SK-N-SH cells

    Energy Technology Data Exchange (ETDEWEB)

    Smets, L.A.; Loesberg, C.; Janssen, M.; Metwally, E.A.; Huiskamp, R.

    1989-06-01

    Radioiodinated m-iodobenzylguanidine (MIBG), an analogue of the neurotransmitter norepinephrine (NE), is increasingly used in the diagnosis and treatment of neural crest tumors. Active uptake and subsequent retention of MIBG and NE was studied in human neuroblastoma SK-N-SH cells. Neuron-specific uptake of (125I)MIBG and (3H)NE saturated at extracellular concentration of 10(-6) M and exceeded by 20-30-fold that by passive diffusion alone. A minimum of 50% of accumulated MIBG remained permanently stored but the SK-N-SH cells were incapable of retaining recaptured (3H)NE. (125I)MIBG was displaced from intracellular binding sites by unlabeled MIBG with 10-fold higher potency than by unlabeled NE. MIBG stored in SK-N-SH cells was insensitive to depletion by the inhibitor of granular uptake reserpine (RSP) and was not precipitated in a granular fraction by differential centrifugation. Only few electron-dense granules were found in these cells by electron microscopy. In contrast, MIBG storage in PC-12 pheochromocytoma cells which contained many storage granules, was sensitive to RSP and part of accumulated drug was recovered in a granular fraction. Accordingly, storage of MIBG in the SK-N-SH neuroblastoma cells is predominantly extravesicular and thus essentially different from that of biogenic amines in normal adrenomedullary tissue or in pheochromocytoma tumors, while sharing with these tissues a common mechanism of active uptake.

  8. Production of coagulation factor VII in human cell lines Sk-Hep-1 and HKB-11.

    Science.gov (United States)

    Corrêa de Freitas, Marcela Cristina; Bomfim, Aline de Sousa; Mizukami, Amanda; Picanço-Castro, Virgínia; Swiech, Kamilla; Covas, Dimas Tadeu

    2017-09-01

    Recombinant factor VII (rFVII) is the main therapeutic choice for hemophilia patients who have developed inhibitory antibodies against conventional treatments (FVIII and FIX). Because of the post-translational modifications, rFVII needs to be produced in mammalian cell lines. In this study, for the first time, we have shown efficient rFVII production in HepG2, Sk-Hep-1, and HKB-11 cell lines. Experiments in static conditions for a period of 96 h showed that HepG2-FVII produced the highest amounts of rhFVII, with an average of 1843 ng/mL. Sk-hep-1-FVII cells reached a maximum protein production of 1432 ng/mL and HKB-11-FVII cells reached 1468 ng/mL. Sk-Hep-1-rFVII and HKB-11-rFVII were selected for the first step of scale-up. Over 10 days of spinner flask culture, HKB-11 and SK-Hep-1 cells showed a cumulative production of rFVII of 152 μg and 202.6 μg in 50 mL, respectively. Thus, these human cell lines can be used for an efficient production of recombinant FVII. With more investment in basic research, human cell lines can be optimized for the commercial production of different bio therapeutic proteins. Copyright © 2017 Elsevier Inc. All rights reserved.

  9. First record of fish microfossils from Ramsåsa, site C, Skåne, southern Sweden

    NARCIS (Netherlands)

    Vergoossen, J.M.J.

    2003-01-01

    Within the thelodont dominated fauna of Ramsåsa, site C, Skåne, southern Sweden (Ludlow, Whitcliffian), the scales of the Thelodus parvidens group outnumber those of the combined T. sculptilis-T. admirabilis groups, some specimens of which are indistinguishable. Scale forms that might belong to one

  10. Clinical fluctuations in MuSK myasthenia gravis are related to antigen-specific IgG4 instead of IgG1

    NARCIS (Netherlands)

    Niks, E H; van Leeuwen, Y; Leite, M I; Dekker, F W; Wintzen, A R; Wirtz, P W; Vincent, A; van Tol, M J D; Jol-van der Zijde, C M; Verschuuren, J J G M

    2008-01-01

    We studied the longitudinal relation between disease severity and titers of antigen-specific IgG subclasses in sera of patients with myasthenia gravis and antibodies to Muscle Specific Kinase (MuSK MG). Six patients were included of whom 55 samples had been collected during 2.5-13.4 years. Anti-MuSK

  11. Application program of CRUST-1 10km continental scientific drilling rig in SK-2 scientific drilling well

    Science.gov (United States)

    Sun, Youhong; Gao, Ke; Yu, Ping; Liu, Baochang; Guo, Wei; Ma, Yinlong; Yang, Yang

    2014-05-01

    SK-2 Well is located in DaQing city,where is site of the largest oil field in China,Heilongjiang province, north-east of China.The objective of SK-2 well is to obtain full cores of cretaceous formation in Song Liao basin,and to build the time tunnel of Cretaceous greenhouse climate change,and to clarify the causes,processes and results of the formations of DaQing oil field. This will ensure to achieve our ultimate goals,to test the CRUST-1 drilling rig and improve China's deep scientific drilling technology,to form the scientific drilling technology,method and system with independent intellectual property rights,and to provide technical knowledge and information for China's ten kilometers super-deep scientific drilling technical resources.SK-2 Well is at 6400 meter depth, where the drilling inclination is 90 degree and the continuous coring length is 3535 meter that from 2865 to 6400 meter,the recovery rate of the core is greater or equal to 95 percent with 100 millimeters core diameter and 3.9 degree per 100 meter geothermal gradient.The CRUST-1 rig is designated with special drilling equipment for continental scientific drilling combined to the oil drilling equipment ability with advanced geological drilling technology which is highly automatic and intelligent. CRUST-1 drilling ability is 10000 meter with the maximum hook load 700 tons, the total power is 4610 Kilowatt.CRUST-1 will be integrated with a complete set of automation equipment,including big torque hydraulic top drive,high accuracy automatic drilling rod feeding system, suspended automatic drill string discharge device,hydraulic intelligent iron roughneck,and hydraulic automatic catwalk to fully meet the drilling process requirements of SK-2.Designed with advanced drilling technique for 260 degree in the bottom of SK-2 well and hard rock,including the drilling tools of high temperature hydraulic hammer,high temperature resistance and high strength aluminum drill pipe,high temperature preparation of mud

  12. [Rhein lysinate induces apoptosis in breast cancer SK-Br-3 cells by inhibiting HER-2 signal pathway].

    Science.gov (United States)

    Lin, Ya-Jun; Huang, Yun-Hong; Zhen, Yong-Zhan; Liu, Xiu-Jun; Zhen, Yong-Su

    2008-11-01

    This study is to investigate the effect of rhein lysinate on inducing human breast cancer cell line SK-Br-3 apoptosis and the role of HER-2 signal pathway in the apoptosis. MTT assay was used to detect SK-Br-3 cell proliferation. Cell cycle and apoptosis were analyzed by flow cytometry. The protein expression and the protein phosphorylation of HER-2 signal pathway were detected by Western blotting. The level of HER-2 mRNA was detected by RT-PCR and the level of HER-2 expression was also detected by immunofluorescence cytochemical methods. The results showed that rhein lysinate remarkably inhibited breast cancer SK-Br-3 cell proliferation. The IC50 value for 48 h treatment was 85 micromol x L(-1). Apoptosis in SK-Br-3 cells was induced by rhein lysinate in a dose dependent manner. The protein expressions of HER-2, NF-KB, and the protein phosphorylation of HER-2 were downregulated, however the protein expression of p53 and p21 was upregulated after rhein lysinate treatment. The level of HER-2 mRNA decreased by using RT-PCR assay and the level of HER-2 expression was also decreased by using immunofluorescence cytochemical assay after rhein lysinate treatment. It can be concluded that rhein lysinate could inhibit SK-Br-3 cell proliferation and induce apoptosis. HER-2/NF-kappaB/p53/p21 signal pathway might be involved in this process. Rhein lysinate has a good prospect to be an adjuvant chemotherapeutic drug.

  13. 海兔素对人乳腺癌SK-BR-3细胞的抑制作用及机制研究%Effect of Aplysin on the Proliferation and Apoptosis in Human Breast Cancer SK-BR-3

    Institute of Scientific and Technical Information of China (English)

    马文龙; 梁惠; 刘颖

    2012-01-01

    本文阐述了海兔素(Aplysin)对人乳腺癌SK-BR-3细胞增殖和凋亡的影响,并探讨了其可能的作用机制.分别采用CCK-8法和流式细胞术Annexin V-FITC/PI双染法测定不同剂量海兔素对SK-BR-3细胞的增殖抑制和凋亡诱导作用,并以Western blot测定SK-BR-3细胞中EGFR、Akt及ERK表达水平和磷酸化水平.结果发现,20、30、40、45、50、55、60 mg/L海兔素处理SK-BR-3细胞24 h后,细胞的生长增殖明显受到抑制,呈量效依赖性,其IC25和IC50值为分别为29.4和33.7 mg/L; IC25和IC50剂量海兔素可明显诱导细胞凋亡,并下调SK-BR-3细胞EGFR、Akt及ERK的磷酸化蛋白表达水平,但是不影响总蛋白表达水平.表明海兔素对人乳腺癌SK-BR-3细胞具有抑制增殖和诱导凋亡的作用,其作用机制可能与海兔素抑制细胞中EGFR蛋白磷酸化,进而阻断下游效应分子Akt和ERK的活化有关.%The effects of aplysin, nature extract from Laurencia, on the proliferation and apoptosis on human breast cancer SK-BR-3 cells were investigated in this study. After treatment with aplysin at a serial of concentrations,cell proliferation was investigated by using Cell Counting Kit-8 and apoptosis was analyzed by Annexin V-FTTC/PI staining via flow cy-tometry.The total and phosphorylated proteins of EGFR.Akt,and ERK were detected by western blot. The results showed that aplysin was able to inhibit SK-BR-3 cell proliferation in a concentration dependent manner within the concentration range of 20,30,40,45,50,55,and 60 mg/L. Aplysin could induce significant apoptosis at the dose of IC25 and IC50. Phosphorylation levels of the receptor of EGFR and cytoplasmic protein of Akt and ERK were significantly down-regulated by aplysin at the dose of IC25 and IC50,while their total protein expression levels were not affected. The results indicated that aplysin could inhibit SK-BR-3 cell proliferation and induce apoptosis by blocking EGFR/Akt and EGFR/ERK signal pathways.

  14. The Inhibiting Effect of Wild Cactus Polysaccharide on Lung Squamous Carcinoma Cells (SK-MES-1)%野生仙人掌多糖对肺鳞癌细胞SK-MES-1生长的抑制作用

    Institute of Scientific and Technical Information of China (English)

    吴迪; 魏斌; 王双; 孙海霞; 辛毅; 张翠丽

    2012-01-01

    目的:探讨仙人掌多糖对体外肺鳞癌细胞(SK-MES-1)生长抑制作用.方法:体外培养SK-MES-1细胞,四甲基偶氮唑盐(MTT)法观察野生仙人掌多糖对其生长的抑制,计算最低抑制浓度及抑制率;观察不同浓度多糖对细胞形态的影响:SDS-PAGE凝胶电泳简析不同组别蛋白质表达的差异.结果:野生仙人掌多糖24 h和48 h对肿瘤细胞的最低抑制浓度和抑制率分别为0.0625 mg/ml.34.06%和0.0625 mg/ml 35.37%;不同组间蛋白质表达有差异.结论:野生仙人掌多糖对SK-MES-1肺鳞癌细胞有抑制作用.%Objective: To investigate the anti-tumor effect of wild Cactus polysaccharide on SK-MES-1 lung squamous carcinoma cells. Methods: SK-MES-1 cells were cultivated in vitro. MTT assay was used to detect the inhibition phenomena of wild Cactus polysaccharide on the growth of tumor cells, calculated the lowest inhibition concentration and tumor inhibitive ratio(IR). The influence of different concentrations of polysaccharide on cell shape was examined respectively. SDS-PAGE assay was used to estimate the difference of protein expression among each group. Results: The lowest inhibition concentration and IR of wild Cactus polysaccharide to SK-MES-1 for 24 h and 48h were 0.0625 mg/ml, 34.06% and 0.0625 mg/ml, 35.37%. The protein expression was different in each group. Conclusion: Wild Cactus polysaccharide had anti-tumor effect on lung squamous carcinoma cells.

  15. Dinámica del color. Puntos de color en el centro, 7+4. Torres junto a la cubierta de la vía ferrea. Torres Centro.Oviedo

    Directory of Open Access Journals (Sweden)

    Río Durán, Fernando

    2003-12-01

    Full Text Available This proyect was the result of a compefition in the city of Oviedo and is a complex of seven fowers on the soufh side and four more towers on the north side of a great plal/orm covering the rail tracks in the city centre. What is most characteristic about this proyect might seem to be its chromatic evolution from the range of reds fo blues, which meant somefhing new in this city, less usedperhaps than others to intense colours; but there are other characteristics which we were concerned with in itsproyection and construction. The colouring of the facades is obtained by the use of resin panels which have the colour included in their ingredients, and which hang at some distance from the interior brick walls, creating a ventilated chamber for the service installafions. In this way the exterior skin of the building is taut and limpid, avoiding the usual presence of tubes and apparatus which the extremepoverty of our building sysfems is normally incapable of concealing.Este proyecto es el resultado de un concurso de arquitectura en la ciudad de Oviedo, se trata de un complejo de siete torres en el lado sur y otras cuafm torres en el lado norte de una gran plataforma que cubre la línea ferroviaria en el centro de la ciudad. Una de las características de este proyecto es su evolución cromáfica del rango de rojos a azules, hecho que significó algo novedoso en esta ciudad no tan usado en otra urbes en cuanto en el uso intenso del color; pero hay otras características en las que estábamos interesados con en su realización. El color de las fachadas se obtiene con el uso de tableros de la resina, prensados, que tienen el color adherido en su composición, dispuestos a poca de distancia de los cerramientos de ladrillo interiores, creando una cámara ventilada utilizada para las instalaciones de servicio. De esta manera la piel exterior del edificio es tersa, limpia y evitando la presencia habitual de conducciones y equipos que la pobreza extrema de

  16. Recall of anti-tobacco advertising and information, warning labels and news stories in a national sample of Aboriginal and Torres Strait Islander smokers.

    Science.gov (United States)

    Nicholson, Anna K; Borland, Ron; Sarin, Jasmine; Wallace, Sharon; van der Sterren, Anke E; Stevens, Matthew; Thomas, David P

    2015-06-01

    To describe recall of anti-tobacco advertising (mainstream and targeted), pack warning labels, and news stories among a national sample of Aboriginal and Torres Strait Islander smokers, and to assess the association of these messages with attitudes that support quitting, including wanting to quit. A quota sampling design was used to recruit participants from communities served by 34 Aboriginal community-controlled health services and one community in the Torres Strait. We surveyed 1643 Aboriginal and Torres Strait Islander smokers from April 2012 to October 2013. Frequency of recall of advertising and information, warning labels and news stories; recall of targeted and local advertising; attitudes about smoking and wanting to quit. More smokers recalled often noticing warning labels in the past month (65%) than recalled advertising and information (45%) or news stories (24%) in the past 6 months. When prompted, most (82%) recalled seeing a television advertisement. Just under half (48%) recalled advertising that featured an Aboriginal or Torres Strait Islander person or artwork (targeted advertising), and 16% recalled targeted advertising from their community (local advertising). Frequent recall of warning labels, news stories and advertising was associated with worry about health and wanting to quit, but only frequent advertising recall was associated with believing that society disapproves of smoking. The magnitude of association with relevant attitudes and wanting to quit increased for targeted and local advertising. Strategies to tackle Aboriginal and Torres Strait Islander smoking should sustain high levels of exposure to anti-tobacco advertising, news stories and warning labels. More targeted and local information may be particularly effective to influence relevant beliefs and subsequently increase quitting.

  17. Microwave Accelerated Transglycosylation of Rutin by Cyclodextrin Glucanotransferase from Bacillus sp. SK13.002

    Directory of Open Access Journals (Sweden)

    Beilei Pan

    2011-06-01

    Full Text Available Rutin was subjected to intermolecular transglycosylation assisted with microwave irradiation using cyclodextrin glucanotransferase (CGTase produced from Bacillus sp. SK13.002. Compared with the conventional enzymatic method for rutin transglycosylation (without microwave irradiation, microwave-assisted reaction (MAR was much faster and thus more efficient. While the conventional reaction took dozens of hours to reach the highest conversion rate of rutin and yield of transglycosylated rutin, MAR of rutin transglycosylation completed within only 6 min providing almost the same conversion rate of rutin and yield of products consisting of mono-, di-, tri-, tetra-, penta-glucosylated rutins. The optimum transglycosylation conditions for microwave irradiation were 40 °C and 60 W with the reaction system consisting mainly of the mixture of 0.3 g rutin (0.49 mmol pre-dissolved in 15 mL methanol, 1.8 g maltodextrin in 15 mL of 0.2 M sodium acetate buffer (pH 5.5 and CGTase (900 U. Results from this study indicated that MAR could be a potentially useful and economical technique for a faster and more efficient transglycosylation of rutin.

  18. Morroniside protects SK-N-SH human neuroblastoma cells against H2O2-induced damage.

    Science.gov (United States)

    Zhang, Jing-Xing; Wang, Rui; Xi, Jin; Shen, Lin; Zhu, An-You; Qi, Qi; Wang, Qi-Yi; Zhang, Lun-Jun; Wang, Feng-Chao; Lü, He-Zuo; Hu, Jian-Guo

    2017-03-01

    Oxidative stress-induced cell injury has been linked to the pathogenesis of neurodegenerative disorders such as spinal cord injury, Parkinson's disease, and multiple sclerosis. Morroniside is an antioxidant derived from the Chinese herb Shan-Zhu-Yu. The present study investigated the neuroprotective effect of morroniside against hydrogen peroxide (H2O2)-induced cell death in SK-N-SH human neuroblastoma cells. H2O2 increased cell apoptosis, as determined by flow cytometry and Hoechst 33342 staining. This effect was reversed by pretreatment with morroniside at concentrations of 1-100 µM. The increase in intracellular reactive oxygen species (ROS) generation and lipid peroxidation induced by H2O2 was also abrogated by morroniside. H2O2 induced a reduction in mitochondrial membrane potential, increased caspase-3 activity, and caused downregulation of B cell lymphoma-2 (Bcl-2) and upregulation of Bcl-2-associated X protein (Bax) expression. These effects were blocked by morroniside pretreatment. Thus, morroniside protects human neuroblastoma cells against oxidative damage by inhibiting ROS production while suppressing Bax and stimulating Bcl-2 expression, thereby blocking mitochondrial-mediated apoptosis. These results indicate that morroniside has therapeutic potential for the prevention and treatment of neurodegenerative diseases.

  19. [Analytical chemistry in works of Maria Skłodowska-Curie].

    Science.gov (United States)

    Hulanicki, Adam

    2012-01-01

    Maria Skłodowska-Curie--a Nobel Prize winner in chemistry--the elements of learning of chemistry gained just by a dint of work of more than ten months in Warsaw in the Institute of Industry and Agriculture Museum. The Nobel Prize concerned a contribution to the progress of chemistry through the discovery of radium and polonium, separation of radium and study of properties of this amazing element. It was awarded for an extremely arduous work, during which the chemical reactions being the principles of analytical chemistry were realized. Unlike to a typical analytical procedure, an initial attempt here was the thousands of kilograms of uranium ore: pitchblende. The final effect was small amounts of new elements: polonium and radium. Both the knowledge and the intuition of the researcher let her have a triumph. The difficulties she experienced because the properties of the searched chemical elements could only be evaluated thanks to the knowledge on other chemical elements. A significant achievement was the determination of the samples by means of radioactivity measurement, which gave rise to radiochemical analytical methods. An extreme analytical precision was demanded in multiple processes of fractional crystallization and precipitation which finally led to the calculation of the atomic mass of radium.

  20. Operation of milling-cutter crushers in the Sukhodol'sk coal preparation plant

    Energy Technology Data Exchange (ETDEWEB)

    Lesikov, A.V.

    1984-04-01

    The Sukhodol'sk coal preparation plant, constructed in 1962 in the Donbass, and with a capacity 4,900 t/d, is equipped with 2 railroad car tippler systems. Coal is supplied in MPS cars. In winter, coal fines supplied to the plant freeze and form large blocks which do not pass through screens separating tipplers from coal hoppers. Breaking up coal blocks is time consuming. From 6 to 18 workers cut coal each day in winter. The Voroshilovgradugleobogashchenie developed the DFM-2 milling cutter for breaking up coal blocks in 1980. In 1982 the system was modified. The cutting drum which consists of 23 discs has a diameter of 750 mm and is 3850 mm wide, revolution rate is 245 rpm. The system is driven by AO2-92-6 and AO2-52-6 electric motors with a rated power of 7.5 kW. The complete milling cutter system consists of 2 units of DFM-2 cutters. The DFM-2 weighs 10.64 t, is 2.3 m long, 3.83 m wide and 1.28 m high. Cutting frozen coal blocks unloaded from a railroad car by means of the DFM-2 system does not take more than 1.5 min. Use of the system eliminates manual operations.

  1. Komposition som projekt i heterogena studentgrupper: Ett exempel från skådespelarutbildningen

    Directory of Open Access Journals (Sweden)

    Sven Bjerstedt

    2015-12-01

    Full Text Available En vanligt förekommande utmaning i högre utbildning är översiktskurser med snäva tidsramar och heterogena studentgrupper. Artikeln vill peka på den positiva potentialen i sådana omständigheter genom att presentera ett konkret exempel på ett arbetssätt som har visat sig framgångsrikt inom skådespelarutbildningens musikundervisning: tonsättningsuppgifter som konceptualiseras individuellt och realiseras gemensamt. I en intervjustudie visas detta arbetssätt ha positiv påverkan på studenternas individuella utveckling i flera avseenden. Bland de läranderesultat som förefaller särskilt intressanta och potentiellt överförbara till åtskilliga ämnesområden finns självförtroende, förmåga att kommunicera samt förmåga att gemensamt hantera en kreativ process.

  2. Developing translational medicine professionals: the Marie Skłodowska-Curie action model.

    Science.gov (United States)

    Petrelli, Alessandra; Prakken, Berent J; Rosenblum, Norman D

    2016-11-29

    End goal of translational medicine is to combine disciplines and expertise to eventually promote improvement of the global healthcare system by delivering effective therapies to individuals and society. Well-trained experts of the translational medicine process endowed with profound knowledge of biomedical technology, ethical and clinical issues, as well as leadership and teamwork abilities are essential for the effective development of tangible therapeutic products for patients. In this article we focus on education and, in particular, we discuss how programs providing training on the broad spectrum of the translational medicine continuum have still a limited degree of diffusion and do not provide professional support and mentorship in the long-term, resulting in the lack of well established professionals of translational medicine (TMPs) in the scientific community. Here, we describe the Marie Skłodowska-Curie Actions program ITN-EUtrain (EUropean Translational tRaining for Autoimmunity & Immune manipulation Network) where training on the Translational Medicine machinery was integrated with education on professional and personal skills, mentoring, and a long-lasting network of TMPs.

  3. Characterization of Probiotic Jelly Drinks with Addition of Lactobacillus plantarum (SK5 from Bekasam during Storage

    Directory of Open Access Journals (Sweden)

    Susi Merry Marini

    2017-02-01

    Full Text Available AbstractLactic acid bacteria from bekasam has potential as an antimicrobial and enzyme producer, as well as probiotics. The purpose of this study were to get the selected formula of the jelly drink with the addition of carrageenan by organoleptic and determine the effect of adding wet biomass (WB and dry biomass (DB Lactobacillus plantarum (SK5 from bekasam against chemical and microbiological parameters jelly beverage probiotics during storage. This study consisted of two phases where a preliminary study to determine the best carrageenan concentration and primary research to determine the chemical and microbiological characteristics jelly drink probiotics during storage. Concentrations of carrageenan used were 0.67%; 1% and 1.34%. The best concentration obtained from the preliminary study was 0.67%. The pH of WB during storage decreased to 2.29; total microbial 0.91 log cfu/g; BAL total of 0.2 log cfu/g; dietary fiber 1.26% and 0.53% total sugars. Dry biomass characterization during storage decreased to pH of 2.54; Microbial total of 3.13 log cfu/g; LAB total of 2.22 log cfu/g, while the increase of the dietary fiber of 2.13% and total sugars 1.96%.

  4. A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillus sp. SK37

    Directory of Open Access Journals (Sweden)

    Yamabhai Montarop

    2011-06-01

    Full Text Available Abstract Background Microbial proteases are one of the most commercially valuable enzymes, of which the largest market share has been taken by subtilases or alkaline proteases of the Bacillus species. Despite a large amount of information on microbial proteases, a search for novel proteases with unique properties is still of interest for both basic and applied aspects of this highly complex class of enzymes. Oxidant stable proteases (OSPs have been shown to have a wide application in the detergent and bleaching industries and recently have become one of the most attractive enzymes in various biotechnological applications. Results A gene encoding a novel member of the subtilase superfamily was isolated from Virgibacillus sp. SK37, a protease-producing bacterium isolated from Thai fish sauce fermentation. The gene was cloned by an activity-based screening of a genomic DNA expression library on Luria-Bertani (LB agar plates containing 1 mM IPTG and 3% skim milk. Of the 100,000 clones screened, all six isolated positive clones comprised one overlapping open reading frame of 45% identity to the aprX gene from Bacillus species. This gene, designated aprX-sk37 was cloned into pET21d(+ and over-expressed in E. coli BL21(DE3. The enzyme product, designated AprX-SK37, was purified by an immobilized metal ion affinity chromatography to apparent homogeneity and characterized. The AprX-SK37 enzyme showed optimal catalytic conditions at pH 9.5 and 55°C, based on the azocasein assay containing 5 mM CaCl2. Maximum catalytic activity was found at 1 M NaCl with residual activity of 30% at 3 M NaCl. Thermal stability of the enzyme was also enhanced by 1 M NaCl. The enzyme was absolutely calcium-dependent, with optimal concentration of CaCl2 at 15 mM. Inhibitory effects by phenylmethanesulfonyl fluoride and ethylenediaminetetraacetic acid indicated that this enzyme is a metal-dependent serine protease. The enzyme activity was sensitive towards reducing agents

  5. Entomological and ecological studies in a new potential zoonotic leishmaniasis focus in Torres Novas municipality, Central Region, Portugal.

    Science.gov (United States)

    Branco, S; Alves-Pires, C; Maia, C; Cortes, S; Cristovão, J M S; Gonçalves, L; Campino, L; Afonso, M O

    2013-03-01

    In Portugal human and canine leishmaniasis are caused by Leishmania infantum, and Phlebotomus perniciosus and P. ariasi are the proven vectors. Three main foci were identified in eighty's decade: Trás-os-Montes and Alto Douro region, Lisbon region and Algarve region, but according to OnLeish observatory data, canine leishmaniasis cases have been reported from several other regions, for which sand fly species and their infection rates are unknown. This study is the first phlebotomine survey in Torres Novas municipality, Santarém District, Portugal. The main objectives were to identify the phlebotomine species, their bioecological aspects, Leishmania infection rate and the risk factors for the presence of phlebotomine species in the municipality. From June to November, 2010, 275 biotopes were surveyed with CDC light-traps. Captures covered the 17 parishes of the municipality and included domestic, peridomestic and sylvatic biotopes. Specimens were identified morphologically and females were used for molecular detection of Leishmania and bloodmeal identification. Simple and multiple logistic regression analysis were used to identify risk factors for phlebotomine presence. Nonparametric tests were used to compare densities of independent groups. A total of 1262 sand flies were captured and identified, and four species detected: P. perniciosus (73.69%), P. ariasi (8.16%), P. sergenti (6.58%) and Sergentomyia minuta (11.57%). In 71.4% localities at least one L. infantum proven vector species was present. Risk factors were identified as: high average temperatures and low relative humidities, sheltered locations and absence of strong wind, presence of pine trees as dominant vegetation, peridomestic biotopes, particularly sheep pens or proximity of sheep, poultry and house martin nests. L. infantum infection rate was 4% for P. ariasi and 0.48% for the total of Larroussius females. P. perniciosus females exhibited an opportunistic behavior, feeding in a wide variety of

  6. Manumycin诱导细胞凋亡抑制乳腺癌细胞SK-BR-3活性%Manumycin inhibits the activity of breast cancer cell line SK-BR-3 via inducing apoptosis

    Institute of Scientific and Technical Information of China (English)

    陶祥; 杨惠玲; 杨林; 郑芹

    2004-01-01

    目的:研究manumycin对乳腺癌腹腔转移癌细胞株SK-BR-3的抑癌效应及其诱导凋亡.方法: 用MTT法检测manumycin对SK-BR-3细胞的抑癌作用.免疫印迹方法检测p38 MAPK蛋白表达.用caspase-3活性检测试剂盒定量检测manumycin诱导细胞凋亡的水平及评估特异性的p38 MAPK抑制剂SB203580对凋亡的影响.结果: 经6 μmol/L、18 μmol/L、54 μmol/L manumycin处理SK-BR-3细胞24 h时,其抑制率分别为(7.4±3.9)%、(21.0±4.4)%和(64.7±4.1)%,呈量效关系.其中后2者的细胞活性与对照组比有显著差异(P<0.01).用药24 h的IC50为42.5 μmol/L.同时此药物可明显增加caspase-3的活性,且这一效应可部分地被p38抑制剂SB203580阻断.免疫印迹结果显示manumycin促进p38的磷酸化.结论:manumycin可通过诱导SK-BR-3细胞凋亡而产生抑癌作用,p38 MAPK是manumycin诱导细胞凋亡的通路之一.

  7. A research on the equipment and use of hydraulic hammer on SK320-6%神钢SK320-6挖掘机液压锤的配置及使用

    Institute of Scientific and Technical Information of China (English)

    翁东玲; 曾利文

    2006-01-01

    系统论证神钢SK320-6履带式液压挖掘机与英格索兰(INGERSOLL-RAND)Ⅵ1200液压锤的配置合理性,分析改装和使用时需注意的事项,从而扩展液压挖掘机在工程实际中的用途.

  8. TaSK5, an abiotic stress-inducible GSK3/shaggy-like kinase from wheat, confers salt and drought tolerance in transgenic Arabidopsis.

    Science.gov (United States)

    Christov, Nikolai Kirilov; Christova, Petya Koeva; Kato, Hideki; Liu, Yuelin; Sasaki, Kentaro; Imai, Ryozo

    2014-11-01

    A novel cold-inducible GSK3/shaggy-like kinase, TaSK5, was isolated from winter wheat using a macroarray-based differential screening approach. TaSK5 showed high similarity to Arabidopsis subgroup I GSK3/shaggy-like kinases ASK-alpha, AtSK-gamma and ASK-epsilon. RNA gel blot analyses revealed TaSK5 induction by cold and NaCl treatments and to a lesser extent by drought treatment. TaSK5 functionally complemented the cold- and salt-sensitive phenotypes of a yeast GSK3/shaggy-like kinase mutant, △mck1. Transgenic Arabidopsis plants overexpressing TaSK5 cDNA showed enhanced tolerance to salt and drought stresses. By contrast, the tolerance of the transgenic plants to freezing stress was not altered. Microarray analysis revealed that a number of abiotic stress-inducible genes were constitutively induced in the transgenic Arabidopsis plants, suggesting that TaSK5 may function in a novel signal transduction pathway that appears to be unrelated to DREB1/CBF regulon and may involve crosstalk between abiotic and hormonal signals.

  9. Effects of SK-896, a new human motilin analogue ([Leu13]motilin-Hse), on postoperative ileus in dogs after laparotomy.

    Science.gov (United States)

    Furuta, Yoshiyuki; Takeda, Motohiro; Nakayama, Yukiharu; Ito, Mikio; Suzuki, Yoshio

    2002-08-01

    The effects of SK-896, a new human motilin analogue ([Leu13]motilin-Hse), on digestive tract motility in postoperative ileus were evaluated in a dog model of ileus after laparotomy. SK-896 was intravenously administered at 0.17, 0.33 and 0.67 microg/kg starting soon after operation and then at 6-h intervals, for a total of 9 times. SK-896 progressively, dose-dependently and significantly increased the duodenal motility from 1 h after operation. The recovery time of the gastrointestinal-interdigestive migrating complex (GI-IMC) activity, which is an indicator of normal gastrointestinal tract activity after laparotomy, was 56.5 +/- 5.0 h in the control group. SK-896 significantly shortened this recovery time. On the other hand, the plasma SK-896 concentrations declined diexponentially after administration, and can be described by a linear pharmacokinetic model within the dose range used. In addition, the pharmacokinetics of SK-896 did not change significantly at any postoperative time. There was no correlation between the plasma SK-896 concentrations and the intensity of duodenal motility, because the activity in the duodenum decreased transiently 13 h after laparotomy and increased with time thereafter. The changes in the activity are considered to reflect the progressive changes in the state of ileus. In conclusion, SK-896 increased the duodenal motility significantly, shortening the recovery time of GI-IMC-like activity in dogs with post-laparotomy ileus. Therefore, it is expected from these results that SK-896 would be useful and effective for the treatment of gastroparalysis after abdominal surgery.

  10. Inhibition of SK4 Potassium Channels Suppresses Cell Proliferation, Migration and the Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.

    Directory of Open Access Journals (Sweden)

    Panshi Zhang

    Full Text Available Treatments for triple-negative breast cancer (TNBC are limited; intermediate-conductance calcium-activated potassium (SK4 channels are closely involved in tumor progression, but little is known about these channels in TNBC. We aimed to investigate whether SK4 channels affect TNBC. First, by immunohistochemistry (IHC and western blotting (WB, increased SK4 protein expression in breast tumor tissues was detected relative to that in non-tumor breast tissues, but there was no apparent expression difference between various subtypes of breast cancer (p>0.05. Next, functional SK4 channels were detected in the TNBC cell line MDA-MB-231 using WB, real-time PCR, immunofluorescence and patch-clamp recording. By employing SK4 specific siRNAs and blockers, including TRAM-34 and clotrimazole, in combination with an MTT assay, a colony-formation assay, flow cytometry and a cell motility assay, we found that the suppression of SK4 channels significantly inhibited cell proliferation and migration and promoted apoptosis in MDA-MB-231 cells (p<0.05. Further investigation revealed that treatment with epidermal growth factor (EGF/basic fibroblast growth factor (bFGF caused MDA-MB-231 cells to undergo the epithelial-mesenchymal transition (EMT and to show increased SK4 mRNA expression. In addition, the down-regulation of SK4 expression inhibited the EMT markers Vimentin and Snail1. Collectively, our findings suggest that SK4 channels are expressed in TNBC and are involved in the proliferation, apoptosis, migration and EMT processes of TNBC cells.

  11. Very high weight ratios of S/K in individual haze particles over Kalimantan during the 1997 Indonesian forest fires

    Science.gov (United States)

    Ikegami, Miwako; Okada, Kikuo; Zaizen, Yuji; Makino, Yukio; Jensen, Jorgen B.; Gras, John L.; Harjanto, Hery

    The elemental composition of individual aerosol particles of 0.15-3 μm radius, collected over Kalimantan during the 1997 Indonesian forest fire event, was analyzed using a transmission electron microscope equipped with an energy-dispersive X-ray analyzer (EDX). Although 60-90% of the particles collected at altitudes of 1-5 km contained K, they exhibited high weight ratios of S/K with median values of 9-18 independent of particle size. These were much larger than those (median values of 2-4) obtained from the forest fires in northern Australia. The high weight ratios over Kalimantan are considered to be due to the heterogeneous growth of particles through the oxidation of SO 2. In addition to SO 2 from the combustion of forest biomass, SO 2 originating from the combustion of peat below the ground is believed to have been important in producing the high S/K ratios.

  12. Genome-wide analysis of KAP1, the 7SK snRNP complex, and RNA polymerase II

    Directory of Open Access Journals (Sweden)

    Ryan P. McNamara

    2016-03-01

    Full Text Available The transition of RNA polymerase II (Pol II from transcription initiation into productive elongation in eukaryotic cells is regulated by the P-TEFb kinase, which phosphorylates the C-terminal domain of paused Pol II at promoter-proximal regions. Our recent study found that P-TEFb (in an inhibited state bound to the 7SK snRNP complex interacts with the KAP1/TRIM28 transcriptional regulator, and that KAP1 and the 7SK snRNP co-occupy most gene promoters containing paused Pol II. Here we provide a detailed experimental description and analysis of the ChIP-seq datasets that have been deposited into Gene Expression Omnibus (GEO: GS72622, so that independent groups can replicate and expand upon these findings. We propose these datasets would provide valuable information for researchers studying mechanisms of transcriptional regulation including Pol II pausing and pause release.

  13. The anti-inflammatory effect of the SOCC blocker SK&F 96365 on mouse lymphocytes after stimulation by Con A or PMA/ionomycin.

    Science.gov (United States)

    Ye, Yanxia; Zhang, Yaxing; Lu, Xiaoyu; Huang, Xiuyan; Zeng, Xiangfeng; Lai, Xinqiang; Zeng, Yaoying

    2011-09-01

    SK&F 96365, 51-(beta-[3-(p-methoxyphenyl)-propyloxy]-p-methoxyphenethyl)-1H-imidazole hydrochloride, has emerged as a useful pharmacological tool in the study of store-operated Ca²⁺ entry (SOCE). But the precise molecular mechanism and effect of SK&F 96365 on mouse lymphocytes are still not well determined. This study investigated the pharmacological profile of SK&F 96365 on mouse lymphocytes stimulated by mitogen concanavalin A (Con A) or by a combination of a protein kinase C (PKC) activator, phorbol 12-myristate 13-acetate (PMA) and a calcium ionophore, ionomycin in vitro. Our results showed that SK&F 96365 pre-treatment diminished the cytosolic calcium rise on lymphocytes induced by ionomycin, PMA/ionomycin, and thapsigargin (TG), respectively. CFDA-SE staining results showed that SK&F 96365 (5-20 μM) inhibited both Con A- and PMA/ionomycin-induced lymphocytes proliferation in a time- and dose-dependent manner. Upon the same stimulation, SK&F 96365 inhibited the expression of CD69 and CD25 on CD3⁺ T lymphocytes in a dose-dependent manner. The cell cycle analyzing results showed that SK&F 96365 caused a G0/G1 phase cell cycle arrest on both Con A- and PMA/ionomycin-activated lymphocytes in a dose-dependent manner. In addition, SK&F 96365 induced a decrease in mitochondrial membrane potential (ΔΨm) and promoted mitochondrial permeability transition (MPT) in both Con A- and PMA/ionomycin-activated lymphocytes. Furthermore, SK&F 96365 significantly inhibited the production of proinflammatory cytokines (interferon (IFN)-γ and tumor necrosis factor (TNF)), and the anti-inflammatory cytokine (IL-10) on both Con A- and PMA/ionomycin-activated lymphocytes. SK&F 96365 did not induce a statistically significant increase in levels of proinflammatory IL-6 and monocyte chemoattractant protein-1 (MCP-1) but of IL-12p70 upon the stimulation of Con A, whereas these three cytokines were markedly inhibited by it upon the stimulation of PMA/ionomycin. This finding

  14. Hafnium-neodymium constraints on source heterogeneity of the economic ultramafic-mafic Noril'sk-1 intrusion (Russia)

    Science.gov (United States)

    Malitch, K. N.; Belousova, E. A.; Griffin, W. L.; Badanina, I. Yu.

    2013-04-01

    The ultramafic-mafic Noril'sk-1 intrusion in Polar Siberia (Russia) hosts one of the world's major platinum-group-element (PGE)-Cusbnd Ni sulphide deposits. In situ Hf-isotope analyses of zircon and baddeleyite, combined with whole-rock Nd-isotope results, identify three distinct clusters of Hfsbnd Nd isotope values restricted to different lithological units (e.g., gabbro-diorite, unmineralised layered sequence gabbros, and mineralised portions, represented by ultramafic and taxitic-textured rocks). Hf-isotope signatures of zircon and baddeleyite from unmineralized mafic rocks (ɛHf from 7.3 ± 1.1 to 11.4 ± 0.3) reflect the dominant role of mantle-derived magmas and suggest that a juvenile mantle was one of the main sources for the ultramafic-mafic Noril'sk-1 intrusion. The less radiogenic Hf-isotope values for zircons from mineralised rocks (ɛHf from 4.9 ± 1.4 to 6.4 ± 1.2) and gabbro-diorite (ɛHf - 1.2 ± 1.9) are indicative of involvement of distinct source components, possibly equivalent to a subcontinental lithospheric mantle and a continental crust, respectively. The significant range in the initial 176Hf/177Hf values of unmineralised rocks and mineralised lithologies indicates interaction of distinct magma sources during formation of the Noril'sk-1 intrusion. Our new findings, in conjunction with whole-rock Nd-isotope results, imply that economic intrusions hosting PGE-Cusbnd Ni deposits of the Noril'sk area have a far more complex magmatic history than is commonly assumed.

  15. The first recorded bloom of Pseudochattonella farcimen (Dictyochophyceae, Heterokonta, (Riisberg I., 2008 in the Gulf of Gdańsk

    Directory of Open Access Journals (Sweden)

    Maria Łotocka

    2009-03-01

    Full Text Available In April 2001 a local bloom of the heterokont phytoflagellate Pseudochattonella farcimen (Riisberg I., 2008 (initially named Chattonella aff. verruculosa was observed for the first time in the southern part of the Gulf of Gdańsk.The species occurred in high cell densities: the count was 11.5 × 106 cells dm-3 and the biomass 927.5 µgC dm-3.

  16. METCAM/MUC18 augments migration, invasion, and tumorigenicity of human breast cancer SK-BR-3 cells.

    Science.gov (United States)

    Zeng, Guofang; Cai, Shaoxi; Liu, Yuan; Wu, Guang-Jer

    2012-01-15

    Previous research has identified METCAM/MUC18, an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family, as a promoter or a suppressor in the development of human breast cancer by MCF7, MDA-MB-231, and MDA-MB-468. To resolve these conflicting results we have investigated the role of this CAM in the progression of the three aforementioned cell lines plus one additional human breast cancer cell line, SK-BR-3. We transfected the SK-BR-3 cells with human METCAM/MUC18 cDNA to obtain G418-resistant clones, which expressed different levels of the protein and which were used to test the effect of human METCAM/MUC18 expression on in vitro motility, invasiveness, anchorage-independent colony formation in soft agar, disorganized growth in a 3D basement membrane culture assay, and in vivo tumorigenesis in athymic nude mice. Enforced METCAM/MUC18 expression increased in vitro motility, invasiveness, and anchorage-independent colony formation of SK-BR-3 cells and favored disorganized growth of the cells in 3D basement membrane culture. Enforced expression also increased tumorigenicity and final tumor weights of SK-BR-3 clones/cells after subcutaneous injection of the cells under the left third nipple of female athymic nude mice. To understand the mechanisms, we also determined the expression of several downstream key effectors in the tumors. Tumor cells from METCAM/MUC18 expressing clones exhibited elevated expression of an anti-apoptotic and survival index (Bcl2), an aerobic glycolysis index (LDH-A), and pro-angiogenesis indexes (VEGF and VAGFR2). We concluded that human METCAM/MUC18 promotes the development of breast cancer cells by increasing an anti-apoptosis and survival pathway and augmenting aerobic glycolysis and angiogenesis.

  17. Enhanced internalization of ErbB2 in SK-BR-3 cells with multivalent forms of an artificial ligand.

    Science.gov (United States)

    Vaidyanath, Arun; Hashizume, Toshihiro; Nagaoka, Tadahiro; Takeyasu, Nao; Satoh, Hitomi; Chen, Ling; Wang, Jiyou; Kasai, Tomonari; Kudoh, Takayuki; Satoh, Ayano; Fu, Li; Seno, Masaharu

    2011-11-01

    Targeting and down-regulation of ErbB2, a member of EGF receptor family, is regarded as one of the key aspect for cancer treatment because it is often overexpressed in breast and ovarian cancer cells. Although natural ligands for ErbB2 have not been found, unlike other ErbB receptors, EC-1, a 20-amino acid circular peptide, has been shown to bind to ErbB2 as an artificial ligand. Previously we showed EC-1 peptide did not induce the internalization of ErbB2 in SK-BR-3 cells. In this report, we designed divalent and multivalent forms of EC-1 peptide with the Fc portion of the human IgG and bionanocapsule modified with ZZ-tag on its surface to improve the interaction with ErbB2. These forms showed higher affinity to ErbB2 than that of EC-1 monomer. Furthermore, prominent endosomal accumulation of ErbB2 occurred in SK-BR-3 cells when stimulated with EC-Fc ligand multivalently displayed on the surface of the bionanocapsule, whereas SK-BR-3 cells as themselves displayed stringent mechanism against ErbB2 internalization without stimulation. The multivalent form of EC-1 peptide appeared to internalize ErbB2 more efficiently than divalent form did. This internalization was unaffected by the inhibition of clathrin association, but inhibited when the cholesterol was depleted which explained either caveolar or GPI-AP-early endocytic compartment (GEEC) pathway. Because of the lack of caveolin-1 expression, caveolar machinery may be lost in SK-BR-3 cell line. Therefore, it is suggested that the multivalent form of EC-1 induces the internalization of ErbB2 through the GEEC pathway.

  18. Lycopene Inhibits Metastasis of Human Liver Adenocarcinoma SK-Hep-1 Cells by Downregulation of NADPH Oxidase 4 Protein Expression.

    Science.gov (United States)

    Jhou, Bo-Yi; Song, Tuzz-Ying; Lee, Inn; Hu, Miao-Lin; Yang, Nae-Cherng

    2017-08-16

    NADPH oxidase 4 (NOX4), with the sole function to produce reactive oxygen species (ROS), can be a molecular target for disrupting cancer metastasis. Several studies have indicated that lycopene exhibited anti-metastatic actions in vitro and in vivo. However, the role of NOX4 in the anti-metastatic action of lycopene remains unknown. Herein, we first confirmed the anti-metastatic effect of lycopene (0.1-5 μM) on human liver adenocarcinoma SK-Hep-1 cells. We showed that lycopene significantly inhibited NOX4 protein expression, with the strongest inhibition of 64.3 ± 10.2% (P Hep-1 cells. We then determined the effects of lycopene on transforming growth factor β (TGF-β)-induced metastasis. We found that TGF-β (5 ng/mL) significantly increased migration, invasion, and adhesion activity, the intracellular ROS level, matrix metalloproteinase 9 (MMP-9) and MMP-2 activities, the level of NOX4 protein expression, and NOX activity. All these TGF-β-induced effects were antagonized by the incubation of SK-Hep-1 cells with lycopene (2.5 μM). Using transient transfection of siRNA against NOX4, we found that the downregulation of NOX4 could mimic lycopene by inhibiting cell migration and the activities of MMP-9 and MMP-2 during the incubation with or without TGF-β on SK-Hep-1 cells. The results demonstrate that the downregulation of NOX4 plays a crucial role in the anti-metastatic action of lycopene in SK-Hep-1 cells.

  19. Análisis del color y el soleamiento en las Torres de Quart de Valencia (España

    Directory of Open Access Journals (Sweden)

    Torres, A.

    2012-09-01

    Full Text Available In the Towers of Quart (s. XV in the city of Valencia (Spain, an exhaustive chromatic measurement has been done of the remains of colour pigment found on the three distinct materials used in the external facade walls. The sun lighting exposure on the building surfaces has also been studied. The paper describes and justifies the process for making an accurate study of the state of the colour and its illumination by the sun, which enables guidelines to be established for renovation works on the monument.

    En las Torres de Quart (s. XV de la ciudad de Valencia, se ha desarrollado un exhaustivo trabajo de medición cromática de los restos de pigmentos existentes en las fachadas extramuros del edificio, sobre tres estratos materiales de distinta naturaleza. Asimismo, se ha estudiado la incidencia de la luz solar sobre las superficies del edificio. Se describe y justifica el proceso llevado a cabo para la obtención de un documento gráfico preciso del estado del color y su iluminación que permita establecer un protocolo de actuación para intervenir en el monumento.

  20. La Torre dei Venti di Atene. Un rilievo in remoto per lo studio e la disseminazione di un orologio archeologico

    Directory of Open Access Journals (Sweden)

    Ramona Quattrini

    2014-07-01

    Full Text Available ItLa Torre dei Venti di Atene è un manufatto di grande rilevanza storica e documentale, significativo anche per l’eccellente stato di conservazione. Mancandone una documentazione di rilievo con tecnologie attuali, è stata scelta come caso studio per verificare fattibilità e performance di un rilievo image-based, lontano dall’edificio e condotto con prese fotografiche non professionali. Gli utilizzi del modello reality-based sono la disseminazione fotorealistica, il confronto con basi di dati scientificamente affidabili e un corpus abilitante per la migliore soluzione di ricostruzione virtuale allo stato originario. In particolare il lavoro si inserisce in un quadro di studi, condotti dal nostro gruppo, nell’ambito della verifica delle rispondenze di alcuni significativi manufatti alle norme e prescrizioni vitruviane.EnThe Tower of Winds (Athens is a very important historical and archaeological monument, very well preserved. In this paper the Tower is presented as a case study, that investigates performance of image-based survey. This survey is performed with current technologies, using non metric and non professional pictures. A specific goal is a reality-based 3D model, enabling photorealistic dissemination and comparison with scientific data and historical surveys. Furthermore we obtained a corpus allowing a correct virtual archaeological reconstruction. This research falls in studies field, carried out by our group, of comparison and check between artifacts and Vitruvian standards.

  1. Buena sangre y hábitos españoles: repensando a Alonso de Silva y Diego de Torres

    Directory of Open Access Journals (Sweden)

    Joanne Rappaport

    2012-01-01

    Full Text Available Este artículo explora la frontera porosa entre mestizos e indígenas en el Nuevo Reino de Granada durante el siglo XVI, interrogándose sobre cómo los mestizos aspirantes a los cacicazgos muiscas se presentaron a sí mismos y cómo fueron caracterizados por sus adversarios en la negociación de sus relaciones con la corona española, los encomenderos locales y sus propios vasallos. La reflexión se centrará, en particular, en dos hombres: don Alonso de Silva, cacique de Tibasosa, y don Diego de Torres, cacique de Turmequé; ambos hijos de mujeres pertenecientes a líneas de sucesión de los cacicazgos muiscas, pero que fueron criados en hogares paternos españoles. Las pretensiones de estos dos personajes sobre los cacicazgos contradecían las aspiraciones de otros mestizos de élite de su tiempo, quienes buscaban aceptación en la sociedad española de Santafé y Tunja. En contraste, los dos se describían sistemáticamente a sí mismos como candidatos  privilegiados para lograr el estatus de miembros de la élite indígena, recurriendo a marcadores de identidad cristianos e ibéricos.

  2. Effect of Citrus floral extracts on the foraging behavior of the stingless bee Scaptotrigona pectoralis (Dalla Torre

    Directory of Open Access Journals (Sweden)

    Julieta Grajales-Conesa

    2012-03-01

    Full Text Available Effect of Citrus floral extracts on the foraging behavior of the stingless bee Scaptotrigona pectoralis (Dalla Torre. Stingless bees have an important role as pollinators of many wild and cultivated plant species in tropical regions. Little is known, however, about the interaction between floral fragrances and the foraging behavior of meliponine species. Thus we investigated the chemical composition of the extracts of citric (lemon and orange flowers and their effects on the foraging behavior of the stingless bee Scaptotrigona pectoralis. We found that each type of flower has its own specific blend of major compounds: limonene (62.9% for lemon flowers, and farnesol (26.5%, (E-nerolidol (20.8%, and linalool (12.7% for orange flowers. In the foraging experiments the S. pectoralis workers were able to use the flower extracts to orient to the food source, overlooking plates baited with hexane only. However, orange flower extracts were seemingly more attractive to these worker bees, maybe because of the particular blend present in it. Our results reveal that these fragrances are very attractive to S. pectoralis, so we can infer that within citric orchards they could be important visitors in the study area; however habitat destruction, overuse of pesticides and the competitive override by managed honeybees might have put at risk their populations and thus the ecological services they provide to us.

  3. Del texto literario a la imagen cinematográfica: humor y terror en La torre de los siete jorobados

    Directory of Open Access Journals (Sweden)

    Mohamed Ben Slama

    2017-08-01

    Full Text Available El presente ensayo estudia dos posturas diferentes a la hora de afrontar el reto de combinar lo humorístico y lo terrorífico en una misma obra: la literaria y la cinematográfica. En La torre de los siete jorobados, tanto el escritor, Emilio Carrere, como el director de cine, Edgar Neville, no renuncian a sus armas al tratar de transmitir a sus respectivos receptores dos sensaciones tan opuestas como son el terror y el humor. Sin embargo, sus enfoques a la hora de combinar estos dos elementos son diferentes: si en la novela el humor está al servicio del terror, en la película se produce un intercambio de papeles: el terror es el que está al servicio del humor. Así pues, el objetivo principal de este estudio es demostrar que la adaptación cinematográfica puede conservar perfectamente el espíritu de la obra original sin, por ello, tener que ser una simple réplica. En esto consiste la labor del cineasta.

  4. Investigation of coupling between chemistry and discharge dynamics in radio frequency hydrogen plasmas in the Torr regime

    Energy Technology Data Exchange (ETDEWEB)

    Kalache, B [LPICM, UMR 7647 (CNRS), Ecole Polytechnique, 91128 Palaiseau Cedex (France); Novikova, T [LPICM, UMR 7647 (CNRS), Ecole Polytechnique, 91128 Palaiseau Cedex (France); Morral, A Fontcuberta i [LPICM, UMR 7647 (CNRS), Ecole Polytechnique, 91128 Palaiseau Cedex (France); Cabarrocas, P Roca i [LPICM, UMR 7647 (CNRS), Ecole Polytechnique, 91128 Palaiseau Cedex (France); Morscheidt, W [LIMHP, UPR 1311 (CNRS), UPN, Av. J. B. Clement, 93430 Villetaneuse (France); Hassouni, K [LIMHP, UPR 1311 (CNRS), UPN, Av. J. B. Clement, 93430 Villetaneuse (France)

    2004-07-07

    We present the results of a study of a capacitively coupled hydrogen discharge by means of a one-dimensional numerical fluid model and experiments. The model includes a detailed description of the gas-phase chemistry taking into account the production of H{sup -} ions by dissociative attachment of H{sub 2} vibrational levels. The population of these levels is described by a Boltzmann vibrational distribution function characterized by a vibrational temperature T{sub V}. The effect of the dissociative-attachment reaction on the discharge dynamics was investigated by varying the vibrational temperature, which was used as a model input parameter. Increasing the vibrational temperature from 1000 to 6000 K affects both the chemistry and the dynamics of the electrical discharge. Because of dissociative attachment, the H{sup -} ion density increases by seven orders of magnitude and the H{sup -} ion density to electron density ratio varies from 10{sup -7} to 6, while the positive ion density increases slightly. As a consequence, the atomic hydrogen density increases by a factor of three, and the sheath voltage drops from 95 to 75 V. Therefore, clear evidence of a strong coupling between chemistry and electrical dynamics through the production of H{sup -} ions is demonstrated. Moreover, satisfactory agreement between computed and measured values of atomic hydrogen and H{sup -} ion densities gives further support to the requirement of a detailed description of the hydrogen vibrational kinetics for capacitively coupled radio frequency discharge models in the Torr regime.

  5. Space and time structure of helium pulsed surface-wave discharges at intermediate pressures (5-50 Torr)

    Science.gov (United States)

    Hamdan, Ahmad; Valade, Fabrice; Margot, Joëlle; Vidal, François; Matte, Jean-Pierre

    2017-01-01

    In this paper, the ignition and development of a plasma created by pulsed surface wave discharges (PSWDs) was experimentally investigated using time-resolved imaging techniques and optical spectroscopy in helium at intermediate gas pressures between 5 and 50 Torr. We found that the ionization front moves at a few km s-1 during the ignition phase and decreases to hundreds of m s-1 after only some tens of µs. Once the plasma has reached a sufficient length, a standing wave pattern is observed in the light emission of the discharge. We attribute its formation to the reflection of the surface wave on the ionization front, which results in a pattern of nodes and antinodes. We have also determined the time and space evolution of the gas temperature. It is shown that the gas temperature increases from the room temperature value to a plateau at several hundreds of degrees over a short time (typically 100 µs). These results supports those obtained by light emission imaging and also show that the standing wave pattern does not affect the gas temperature.

  6. Plain packaging implementation: perceptions of risk and prestige of cigarette brands among Aboriginal and Torres Strait Islander people.

    Science.gov (United States)

    Maddox, Raglan; Durkin, Sarah; Lovett, Ray

    2016-06-01

    To assess the impact of plain packaging with larger graphic health warnings on perceptions of risk and prestige related to different cigarette brands among Aboriginal and Torres Strait Islander people in the Australian Capital Territory. We hypothesised that the changes would decrease perceptions that 'some cigarette brands are more harmful than others', and that 'some brands are more prestigious than others', and this would be stronger among participants aged ≤35 years, and among smokers compared with non-smokers. Participants completed the survey prior to packaging changes, and were followed up 12 months later (n=98). Repeated measures ANCOVAs assessed perception changes. Following plain packaging implementation, there was a significant reduction in perceptions that 'some cigarette brands are more harmful than others'. There was no overall change in perceptions of prestige. However, there was a significant interaction for age. Analyses indicated a reduction in perceptions that 'some cigarette brands are more prestigious than others' among younger participants (p=0.05), but no change among older participants (p>0.20). There was no interaction for smoking status for perceptions of prestige, indicating smokers' and non-smokers' perceptions did not differ on this measure. These findings provide support for the packaging changes. © 2015 Public Health Association of Australia.

  7. Lékařský software pro vyúčtování dokladů

    OpenAIRE

    Pohl, Ondřej

    2013-01-01

    Cílem práce je rozšíření lékařského softwaru OpenDositech.Rozšířením je implementace rozhraní pro elektronickou komunikaci s webovými portály zdravotních pojišťoven do programu OpenDositech. Nejprve je popsán dostupný lékařský software spolu s datovým rozhraním pro předávání lékařských dokladů zdravotním pojišťovnám. Práce se poté zabývá předáváním dokladů přes Portál zdravotních pojišťoven. V závěru práce je popsána implementace rozhraní do programu OpenDositech. The aim of this thesis is...

  8. Role of degassing of the Noril'sk nickel deposits in the Permian-Triassic mass extinction event.

    Science.gov (United States)

    Le Vaillant, Margaux; Barnes, Stephen J; Mungall, James E; Mungall, Emma L

    2017-03-07

    The largest mass extinction event in Earth's history marks the boundary between the Permian and Triassic Periods at circa 252 Ma and has been linked with the eruption of the basaltic Siberian Traps large igneous province (SLIP). One of the kill mechanisms that has been suggested is a biogenic methane burst triggered by the release of vast amounts of nickel into the atmosphere. A proposed Ni source lies within the huge Noril'sk nickel ore deposits, which formed in magmatic conduits widely believed to have fed the eruption of the SLIP basalts. However, nickel is a nonvolatile element, assumed to be largely sequestered at depth in dense sulfide liquids that formed the orebodies, preventing its release into the atmosphere and oceans. Flotation of sulfide liquid droplets by surface attachment to gas bubbles has been suggested as a mechanism to overcome this problem and allow introduction of Ni into the atmosphere during eruption of the SLIP lavas. Here we use 2D and 3D X-ray imagery on Noril'sk nickel sulfide, combined with simple thermodynamic models, to show that the Noril'sk ores were degassing while they were forming. Consequent "bubble riding" by sulfide droplets, followed by degassing of the shallow, sulfide-saturated, and exceptionally volatile and Cl-rich SLIP lavas, permitted a massive release of nickel-rich volcanic gas and subsequent global dispersal of nickel released from this gas as aerosol particles.

  9. Reflection seismic studies over the end-glacial Burträsk fault, Skellefteå, Sweden

    Directory of Open Access Journals (Sweden)

    C. Juhlin

    2011-01-01

    Full Text Available Reflection seismic data were acquired along a ca. 22 km long profile over the end-glacial Burträsk fault with a nominal receiver and source spacing of 20 m. A steeply dipping reflection can be correlated to the Burträsk fault, indicating that the fault dips at about 55° to the southeast near the surface. The reflection from the fault is rather poorly imaged, probably due to a lateral offset in the fault of about 1 km at this location and the crookedness of the seismic profile in the vicinity of the fault. A more pronounced steeply dipping reflection is observed about 4 km southeast of the Burträsk fault. Based on its correlation with a topographic low at the surface this reflection is interpreted to originate from a fracture zone. There are no signs of large displacements along this zone as the glacial ice receded, but earthquakes could be associated with it today. Other reflections on the processed seismic section may originate from changes in lithological variations in the supra-crustal rocks or from intrusions of more mafic rock. Constraints on the fault geometry provided by the reflection seismic data will help determine what stresses were required to activate the fault when the major rupture along it occurred ca. 9500 years ago.

  10. East Scandinavian and Noril'sk plume mafic large igneous provinces of Pd-Pt ores: Geological and metallogenic comparison

    Science.gov (United States)

    Mitrofanov, F. P.; Bayanova, T. B.; Korchagin, A. U.; Groshev, N. Yu.; Malitch, K. N.; Zhirov, D. V.; Mitrofanov, A. F.

    2013-09-01

    This paper compares the geological, geophysical, and isotopic geochemical data on the Paleoproterozoic East Scandinavian Pd-Pt province in the Baltic Shield and the Late Paleozoic Noril'sk Pd-Pt province in the Siberian Craton. Both provinces contain large magmatic PGE deposits: low-sulfide in the Baltic Shield and high-sulfide in the Siberian Craton. Multidisciplinary evidence shows that the East Scandinavian mafic large igneous province, which has a plume nature, is intracratonic and was not subjected to the crucial effect of subduction-related and other contamination processes, whereas the Noril'sk province is pericratonic with substantial crustal contamination of the intrusive processes. Low-sulfide Pd-Pt deposits dominate in the East Scandinavian province, while high-sulfide Ni-Cu-PGE deposits play the leading role in the Noril'sk province. The U-Pb, Sm-Nd, and Rb-Sr isotopic data indicate multistage and long-term (tens of millions of years) geological history of mafic large igneous provinces. The plume magmatism with specific geochemistry and metallogeny is probably related to lower mantle sources.

  11. Inhibitory Effects of Anti-sense PTTG on Malignant Phenotype of Human Ovarian Carcinoma Cell Line SK-OV-3

    Institute of Scientific and Technical Information of China (English)

    陈刚; 李静; 李辅军; 李箫; 周剑锋; 卢运萍; 马丁

    2004-01-01

    To construct eukaryotic expression vector expressing full length anti-sense pituitary tumor transforming gene (PTTG) mRNA and observe its blocking effect on the potential invasion of human ovarian carcinoma cell line SK-OV-3. PCR primers containing designed enzyme cut sites were used for cloning full-length PTTG gene fragment, and the resulting PCR product was inserted into the eukaryotic vector pcDNA3. 1 in the antisense direction. The recombinant vector was then transfected into SK-OV-3 by Lipofectamine. The positive cell clone was screened by G418, PTTG and bFGF at protein level expression were detected by Western blot. The biological behavior change of transfection positive cells was observed by colony formation in soft agar assay. Our results showed that SK-OV-3 clones stably expressing full-length recombinant pcDNA3. 1-PTTGas were obtained. The expressions of PTTG and bFGF protein in transfected cells were decreased by 61.5 % and 52.3%, respectively as compared with non-transfected ones. The number of colony formation was reduced significantly in transfected cells as compared with empty vector transfected and non-transfected cells. It is concluded that the recombinant vector pcDNA3. 1-PTTGas is a novel tool and provides an alternative anti-sense gene therapy targeted at PTTG in human carcinoma.

  12. Hepatotoxicity after liver irradiation in children and adolescents. Results from the RiSK

    Energy Technology Data Exchange (ETDEWEB)

    Roesler, Pascal; Christiansen, Hans [Medical School Hannover, Department of Radiotherapy and Special Oncology, Hannover (Germany); Kortmann, Rolf-Dieter [University of Leipzig, Department of Radiotherapy, Leipzig (Germany); Martini, Carmen [University Hospital of Freiburg, Department of Radiotherapy, Freiburg im Breisgau (Germany); Matuschek, Christiane [Heinrich-Heine University of Duesseldorf, Department of Radiotherapy, Medical Faculty, Duesseldorf (Germany); Meyer, Frank [Hospital Augsburg, Department of Radiotherapy, Augsburg (Germany); Ruebe, Christian [University Hospital of Homburg/Saar, Department of Radiotherapy, Homburg/Saar (Germany); Langer, Thorsten [University Hospital of Schleswig-Holstein, Department of Pediatrics, Pediatric Oncology, Campus Luebeck (Germany); Koch, Raphael [University of Muenster, Institute of Biostatistics and Clinical Research, Muenster (Germany); Eich, Hans Theodor; Willich, Normann [University Hospital of Muenster, Department of Radiotherapy, Muenster (Germany); Steinmann, Diana [Medical School Hannover, Department of Radiotherapy and Special Oncology, Hannover (Germany); University Hospital of Muenster, Department of Radiotherapy, Muenster (Germany)

    2015-05-01

    The aim of this study was to evaluate acute and late radiotherapy-associated hepatotoxicity in consideration of dose-volume effects and liver function in childhood and adolescence. Since 2001, irradiated children and adolescents in Germany have been prospectively documented in the ''Register of Treatment-Associated Late Effects After Radiotherapy of Malignant Diseases in Childhood and Adolescence (RiSK)'' using standardized forms. Toxicity was graded according to the Radiation Therapy Oncology Group (RTOG) criteria. Until April 2012, 1,392 children and adolescents from 62 radiotherapy centers were recruited. In all, 216 patients underwent irradiation of the liver (median age 9 years, range 1-18 years, 70 patients with total-body irradiation, TBI). For 75 % of patients without TBI, information on acute toxicity of the liver was available: 24 patients had acute toxicity of grade 1-4 (grade 1, 2, and 4, in 20, 3, and 1 patient, respectively), including five patients receiving simultaneous hepatotoxic chemotherapy. Information on late toxicity was documented in 465 forms from 216 patients, with a median follow-up of 2 years. A maximum grade of toxicity of ≥ 0 occurred in 18 patients over time (with grade 1, 2, and 3 toxicity occurring in 15, 2, and 1 patient, respectively), including three patients (17 %) with TBI. One of them received simultaneous hepatotoxic chemotherapy. In multivariable analysis, volume-dose correlations showed no statistically noticeable effect on acute or chronic toxicity. Only low hepatotoxicity developed in children after irradiation of various abdominal and thoracic tumors. Due to the low radiation doses to the liver (median liver dose = 5 Gy) and the low toxicities that were consecutively observed, dose-volume curves for liver toxicity could not be established. These findings reflect the cautious attitude of radiation oncologists in terms of attributable liver doses in the treatment of the investigated tumor entities. It

  13. Intracorneal Rings (INTACS SK) Might be Beneficial in Keratoconus; A Prospective Nonrandomized Study.

    Science.gov (United States)

    A Ibrahim, Tarek; Elmor, Osama

    2013-01-01

    In order to determine the effect of intracorneal rings (Intacs SK), when implanted in keratoconic patients, on corneal curvature, Uncorrected Visual Acuity (UCVA), Best Corrected Visual Acuity (BCVA) and on the progression of the cone through three years follow-up period. In this prospective nonrandomized study 114 eyes of 71 keratoconic patients (38 females and 33 males) were implanted with Intacs SK. Incisions were always made in the steep meridian. UCVA, BCVA, Corneal Topography (TMS) were measured pre and postoperatively and at intervals of 1, 3, 6 & 12 months then yearly for 3 consecutive years. Preoperative mean k-reading was 52.53 and 48.18, 49.56, 49.17, 48.51, 48.15 & 48.01 at 1, 3, 6, 12, 24 & 36 months postoperatively (P‹0.01). In terms of UCVA, 15.64% of patients gained more than 3 lines and 69.73% gained 1-3 lines with a total of 85.37% of patients gaining lines compared to their preoperative UCVA (P‹0.01) while 14.63% of cases did not gain any lines at 1 month postoperative. At three months postoperatively, 12.64% gained more than 3 lines, 71.15% gained 1-3 lines with a total of 83.79% while 16.21% did not gain any lines. Three years postoperative 11.82% of cases gained more than 3 lines, 73.23% gained 1-3 lines with a total of 85.05% while 14.95% did not gain any lines (P‹0.01). With regard to BCVA, 19.73% gained more than 3 lines, 68.26% gained 1-3 lines with a total of 87.99% of cases gaining lines compared to their preoperative BCVA (P‹0.01) while 12.01% did not gained any lines at 1 month postoperative. At three months postoperatively, 14.96% gained more than 3 lines, 70.19% gained 1-3 lines with a total of 85.15% while 14.85% did not gain any lines. Three years postoperative, 12.17% gained more than 3 lines, 71.78% gained 1-3 lines with a total of 83.95% (P‹0.01) while 16.05% did not gain any lines. No eyes lost any lines as it pertained to UCVA & BCVA. Despite the fluctuation of k-readings, UCVA and BCVA in the first 3 months, which

  14. [Maria Skłodowska-Curie and Piotr Curie an epoch-makingin year 1898].

    Science.gov (United States)

    Wielogórski, Zbigniew

    2012-01-01

    For many reasons the year 1898 was unusual for Maria Skłodowska-Curie and her husband. After defining the subject of the doctoral thesis and choosing Henri Becqerel as thesis supervisor, Maria started intensive experimental work. In the allotted room called storeroom, in conditions that were far too inadequate, they managed to put up a unique measuring equipment composed of instruments whose originator was Pierre Curie. In the ionization chamber and in the piezoelectric quartz charges formed, whose mutual neutralization was shown by the quadrant electrometer. Ionization current, which was measured quantitatively, was proportional to the radiation of the sample. Studying many elements, their compounds and minerals enabled Maria to state that uranium is not the only element endowed with the power of radiation; the second one turned out to be thorium. Anomaly detected in the radiation of uranium minerals made it possible for Maria to draw an extremely important conclusion: radioactive uranium and thorium are not the only elements endowed with such an attribute. Pitchblende, which was studied by the Curie couple, had to contain also other radioactive substances. Gustave Bémont also participated in the chemical analysis of the uranium ore and it is worth reminding that he was involved in the discovery of polonium and uranium. The phenomenon of radioactivity couldn't have been explained if it was not for the sources of strong radioactivity. Those sources undoubtedly could have been the discovered elements but their scanty content in the uranium ore made their isolation very difficult and laborious. Access to industrial remains after procession of pitchblende from Jachymov (Sankt Joachimstahl), obtained owing to the mediation of Eduard Suess, provided the source of this raw material. From it, in a shack also called le hangar, the Curie couple isolated the first samples of the radium salt. This element, later extracted by discoverers on a grand scale and handed over in a

  15. Bioelectrochemical Mn(II) leaching from manganese ore by Lactococcus lactis SK071115.

    Science.gov (United States)

    Jeon, Bo Young; Park, Doo Hyun

    2011-02-01

    L. lactis sk071115 has been shown to grow more actively and generate lower levels of lactate in glucose-defined medium with nitrate than in medium with Mn(IV). By adding Mn(IV) to a L. lactis culture, lactate production was relatively reduced in combination with Mn(II) production, but cell mass production levels did not increase. Both cell-free extract and intact L. lactis cells reacted electrochemically with Mn(IV) but did not react with Mn(II) upon cyclic voltammetry using neutral red (NR) as an electron mediator. A modified graphite felt cathode with NR (NR-cathode) was employed to induce electrochemical reducing equivalence for bacterial metabolism. Cell-free L. lactis extract catalyzed the reduction of Mn(IV) to Mn(II) under both control and electrochemical reduction conditions; however, the levels of Mn(II) generated under electrochemical reduction conditions were approximately 4 times those generated under control conditions. The levels of Mn(II) generated by the catalysis of L. lactis immobilized in the NR-cathode (L-NR-cathode) under electrochemical reduction conditions were more than 4 times that generated under control conditions. Mn(II) production levels were increased by approximately 2.5 and 4.5 times by the addition of citrate to the reactant under control and electrochemical reduction conditions, respectively. The cumulative Mn(II) produced from manganese ore by catalysis of the L-NR-cathode for 30 days reached levels of approximately 3,800 and 16,000 mg/l under control and electrochemical reduction conditions, respectively. In conclusion, the electrochemical reduction reaction generated by the NR-cathode activated the biochemical reduction of Mn(IV) to Mn(II) by L. lactis.

  16. Effect of rhaponticin on apoptosis in human breast cancer SK-BR-3 cells%土大黄苷对人乳腺癌细胞SK-BR-3凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    赵素容; 刘浩; 梁颖; 钱江华; 陈超; 蒋志文

    2012-01-01

    目的:观察土大黄苷对人乳腺癌细胞SK-BR-3凋亡的影响.方法:Annexin V-FITC/PI双标法检测100、200、400 μmol/L土大黄苷对SK-BR-3细胞凋亡的影响,Western blot法检测Bcl-2及Bax蛋白的表达情况,试剂盒检测caspase-3的活性变化.结果:土大黄苷可诱导SK-BR-3细胞出现明显的早期凋亡,并呈浓度依赖性(P<0.05~P<0.01).土大黄苷在100、200、400 μmol/L浓度时均可抑制Bcl-2蛋白的表达,且随着其浓度的增加,蛋白表达逐渐降低,并促进Bax蛋白的表达(P<0.01).土大黄苷对caspase-3具有激活作用,且随着土大黄苷浓度的增加,caspase-3的活化程度逐渐增强.结论:土大黄苷具有诱导乳腺癌细胞凋亡的作用,其机制可能与调节凋亡相关蛋白Bcl-2、Bax的表达及激活caspase-3有关(P<0.01).%Objective: To investigate the effect of rhaponticin on apoptosis in human breast cancer SK-BR-3 cells. Methods: SK-BR-3 cells were treated with rhaponticin at the concentrations of 100,200,and 400 ?mol/L. Apoptosis was examined with Annexin V-FITC Apoptosis Detection Kit by flow cytometry. The expressions of Bcl-2 and Bax protein were analyzed by Western blot, caspase-3 activity was detected by special kit. Results: Rhaponticin induced obvious early apoptosis in SK-BR-3 cells in a concentration-dependent manner( P < 0. 05 to P < 0. 01) . Western blot results showed that rhaponticin enhanced the expression of Bax protein, and inhibited the expression of Bcl-2 protein, which was gradually down-regulated following the increase of rhaponticin's concentration ( P < 0. 01 ). Meanwhile, rhaponticin induced the activation of caspase-3 , and caspase-3 activity was increased with its concentration ( P < 0. 01 ) . Conclusions: Rhaponticin can induce apoptosis in SK-BR-3 cells, which may be correlated to the down-regulation of Bcl-2, the up-regulation of Bax, and the activation of caspase-3.

  17. Direct Host Plasminogen Binding to Bacterial Surface M-protein in Pattern D Strains of Streptococcus pyogenes Is Required for Activation by Its Natural Coinherited SK2b Protein.

    Science.gov (United States)

    Chandrahas, Vishwanatha; Glinton, Kristofor; Liang, Zhong; Donahue, Deborah L; Ploplis, Victoria A; Castellino, Francis J

    2015-07-24

    Streptokinase (SK), secreted by Group A Streptococcus (GAS), is a single-chain ∼47-kDa protein containing three consecutive primary sequence regions that comprise its α, β, and γ modules. Phylogenetic analyses of the variable β-domain sequences from different GAS strains suggest that SKs can be arranged into two clusters, SK1 and SK2, with a subdivision of SK2 into SK2a and SK2b. SK2b is secreted by skin-tropic Pattern D M-protein strains that also express plasminogen (human Pg (hPg)) binding Group A streptococcal M-protein (PAM) as its major cell surface M-protein. SK2a-expressing strains are associated with nasopharynx tropicity, and many of these strains express human fibrinogen (hFg) binding Pattern A-C M-proteins, e.g. M1. PAM interacts with hPg directly, whereas M1 binds to hPg indirectly via M1-bound hFg. Subsequently, SK is secreted by GAS and activates hPg to plasmin (hPm), thus generating a proteolytic surface on GAS that enhances its dissemination. Due to these different modes of hPg/hPm recognition by GAS, full characterizations of the mechanisms of activation of hPg by SK2a and SK2b and their roles in GAS virulence are important topics. To more fully examine these subjects, isogenic chimeric SK- and M-protein-containing GAS strains were generated, and the virulence of these chimeric strains were analyzed in mice. We show that SK and M-protein alterations influenced the virulence of GAS and were associated with the different natures of hPg activation and hPm binding. These studies demonstrate that GAS virulence can be explained by disparate hPg activation by SK2a and SK2b coupled with the coinherited M-proteins of these strains.

  18. [Connection of magnetic antisense probe with SK-Br-3 oncocyte mRNA nucleotide detected by high resolution atomic force microscope].

    Science.gov (United States)

    Tan, Shude; Ouyang, Yu; Li, Xinyou; Wen, Ming; Li, Shaolin

    2011-06-01

    The present paper is aimed to detect superparamagnetic iron oxide labeled c-erbB2 oncogene antisense oligonucleotide probe (magnetic antisense probe) connected with SK-Br-3 oncocyte mRNA nucleotide by high resolution atomic force microscope (AFM). We transfected SK-Br-3 oncocyte with magnetic antisense probe, then observed the cells by AFM with high resolution and detected protein expression and magnetic resonance imagine (MRI). The high resolution AFM clearly showed the connection of the oligonucleotide remote end of magnetic antisense probe with the mRNA nucleotide of oncocyte. The expression of e-erbB2 protein in SK-Br3 cells were highly inhibited by using magnetic antisense probe. We then obtained the lowest signal to noise ratio (SNR) of SK-Br-3 oncocyte transfected with magnetic antisense probe by MRI (PSK-Br-3 mRNA of tumor cell nuclear.

  19. Pääsküla sõjaväeobjekti jääkreostus ning piirkonna edasise kasutamise perspektiivid / Triin Uudeväli, Anto Raukas

    Index Scriptorium Estoniae

    Uudeväli, Triin

    2004-01-01

    Pääskülas asunud NL-i raketijuhtimisekeskuse ja sideväeosa poolt tekitatud keskkonnareostuse hindamisest, likvideerimisest ning ettepanekutest territooriumi edasiseks kasutamiseks. Kaart. Skeem. Fotod

  20. Kit-negative fibroblast-like cells expressing SK3, a Ca2+-activated K+ channel, in the gut musculature in health and disease

    DEFF Research Database (Denmark)

    Vanderwinden, Jean-Marie; Rumessen, Jüri J; de Kerchove d'Exaerde, Alban;

    2002-01-01

    CD34-ir fibroblast-like cells adjacent to, but distinct from, the cells of Cajal remains elusive. The distribution of SK3 was studied by immunohistochemistry in the normal human gut, in motility disorders with a lack of cells of Cajal (infantile hypertrophic pyloric stenosis and Hirschsprung...... were lacking. These cells were CD34-ir fibroblast-like cells in the human gut and in the mouse stomach, while SK3-ir cells in the mouse intestine were CD34 negative. As SK channels are reportedly involved in inhibitory neurotransmission, our morphological observations suggest that SK3-ir interstitial...... cells, distinct from the Kit-ir interstitial cells of Cajal, may represent a novel cellular component in the control of excitability of the digestive musculature. Further studies will be required to directly address the function of these cells....

  1. The Mitochondria-Targeted Antioxidant SkQ1 Downregulates Aryl Hydrocarbon Receptor-Dependent Genes in the Retina of OXYS Rats with AMD-Like Retinopathy

    Directory of Open Access Journals (Sweden)

    M. L. Perepechaeva

    2014-01-01

    Full Text Available The mitochondria-targeted antioxidant SkQ1 is a novel drug thought to retard development of age-related diseases. It has been shown that SkQ1 reduces clinical signs of retinopathy in senescence-accelerated OXYS rats, which are a known animal model of human age-related macular degeneration (AMD. The aim of this work was to test whether SkQ1 affects transcriptional activity of AhR (aryl hydrocarbon receptor and Nrf2 (nuclear factor erythroid 2-related factor 2, which are considered as AMD-associated genes in the retina of OXYS and Wistar rats. Our results showed that only AhR and AhR-dependent genes were sensitive to SkQ1. Dietary supplementation with SkQ1 decreased the AhR mRNA level in both OXYS and Wistar rats. At baseline, the retinal Cyp1a1 mRNA level was lower in OXYS rats. SkQ1 supplementation decreased the Cyp1a1 mRNA level in Wistar rats, but this level remained unchanged in OXYS rats. Baseline Cyp1a2 and Cyp1b1 mRNA expression was stronger in OXYS than in Wistar rats. In the OXYS strain, Cyp1a2 and Cyp1b1 mRNA levels decreased as a result of SkQ1 supplementation. These data suggest that the Cyp1a2 and Cyp1b1 enzymes are involved in the pathogenesis of AMD-like retinopathy of OXYS rats and are possible therapeutic targets of SkQ1.

  2. Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3 in rat uterus after stimulation with 17β-estradiol.

    Directory of Open Access Journals (Sweden)

    Mette Rahbek

    Full Text Available Preterm births accounts for roughly 9% of all births worldwide and can have detrimental or even lethal consequences for the infant. However to develop new treatment that will lower the rate of preterm births, more knowledge is required on the factors contributing to the contraction and relaxation of the myometrium. The small conductance Ca²⁺-activated potassium channel subtype 3 (SK3 has been identified in the myometrium of several species including humans, mice and rats, but with great inter species variation of the expression pattern and regulation. The aim of this study was to investigate the expression of SK3 in the uterus of rats stimulated with 17β-estradiol and progesterone in order to get an in depth understanding of the rat uterine SK3. Using immunohistochemistry SK3 was localized to the glandular and luminal endometrial lamina epitheliali. Furthermore, a weak signal was observed in the myometrium. Using Western blot the protein level of SK3 was found to increase in uteri from animals treated with 17β-estradiol, an effect that was not reflected at the mRNA level. The levels of mRNA for SK3 were significantly lower in the uterus of 17β-estradiol-treated animals than in the uterus of ovariectomized animals. We conclude that the SK channels are present in the endometrial epithelium, and possibly also in the myometrium of the rat uterus. Furthermore, the hormonal effect on SK3 caused by 17β-estradiol includes divergent regulation at mRNA and protein levels.

  3. The study of pressure measurement techniques and devices in the range of 10(exp -1) to 10(exp -5) torr (2 millipsi to 0.2 micropsi)

    Science.gov (United States)

    Hoffman, John H.

    1990-01-01

    The atmospheric pressure range was studied in a region where conventional pressure sensing devices do not provide meaningful measurements. However, a hot filament gauge was developed and miniaturized which will measure the pressure in the 10(exp -1) to 10(exp -5) torr (2 millipsi to 0.2 micropsi) region, hence the name Micropsi gauge. Laboratory studies were made comparing the currently available devices with the newly developed miniature low power 'Micropsi' pressure sensor.

  4. Alcohol-Related Violence among the Australian Aboriginal and Torres Strait Islanders of the Northern Territory: Prioritizing an Agenda for Prevention-Narrative Review Article.

    OpenAIRE

    Ramya Ramamoorthi; Rama Jayaraj; Leonard Notaras; Mahiban Thomas

    2014-01-01

    Abstract Alcohol - related violence among Australian Aboriginal and Torres Strait Islanders (also called as “Indigenous”) is a major public health concern in Northern Territory of Australia. There is dearth of epidemiological data that link three contributing epidemics: alcohol misuse, violence, and trauma in the Northern Territory. In this review, we aimed to concentrate on how these epidemics intersect among the Indigenous people in the Northern Territory. In our descriptive review, we have...

  5. Dos políticas de instrumental científico: el Instituto del Material científico y el Torres Quevedo

    Directory of Open Access Journals (Sweden)

    Romero, Ana

    1998-08-01

    Full Text Available Not available.En este artículo se comparan las políticas públicas de adquisición, distribución y mantenimiento de material científico para centros docentes y de investigación, a través de dos instituciones: el Instituto del Material Científico (1911-1936 y el Instituto Torres Quevedo (1939-1965.

  6. Cuerpo y torres de la iglesia de Villaconejos ¿una nueva obra de fray Alberto de la Madre de Dios?

    Directory of Open Access Journals (Sweden)

    Martínez Ruiz, Javier

    2014-03-01

    Full Text Available Based on unpublished documentation, the author suggests that Friar Albert of the Mother of God is the architect of the main body and towers of the church of Villaconejos.Argumentos que apuntan a fray Alberto de la Madre de Dios como tracista del cuerpo y torres de la iglesia de Villaconejos, partiendo de la documentación inédita de la obra.

  7. Data on in vivo selection of SK-OV-3 Luc ovarian cancer cells and intraperitoneal tumor formation with low inoculation numbers

    Directory of Open Access Journals (Sweden)

    Elly De Vlieghere

    2016-03-01

    Full Text Available This data paper contains information about the in vivo model for peritoneal implants used in the paper “Tumor-environment biomimetics delay peritoneal metastasis formation by deceiving and redirecting disseminated cancer cells” (De Vlieghere et al., 2015 [1]. A double in vivo selection of SK-OV-3 Luc human ovarian cancer cell line was used to create SK-OV-3 Luc IP1 and SK-OV-3 Luc IP2 cell lines. This data paper shows functional activities of the three cell lines in vitro and in vivo. Phase-contrast images show the morphology of these cells, metabolic and luciferase activity has been determined. Survival data of mice peritoneally injected with SK-OV-3 Luc or SK-OV-3 Luc IP2 is available with H&E histology of the peritoneal implants. Tumor growth curves and bioluminescent images of mice inoculated with a different number of SK-OV-3 Luc IP2 cells are also included.

  8. Calcium-activated potassium channel SK1 is widely expressed in the peripheral nervous system and sensory organs of adult zebrafish.

    Science.gov (United States)

    Cabo, R; Zichichi, R; Viña, E; Guerrera, M C; Vázquez, G; García-Suárez, O; Vega, J A; Germanà, A

    2013-10-25

    Sensory cells contain ion channels involved in the organ-specific transduction mechanisms that convert different types of stimuli into electric energy. Here we focus on small-conductance calcium-activated potassium channel 1 (SK1) which plays an important role in all excitable cells acting as feedback regulators in after-hyperpolarization. This study was undertaken to analyze the pattern of expression of SK1 in the zebrafish peripheral nervous system and sensory organs using RT-PRC, Westernblot and immunohistochemistry. Expression of SK1 mRNA was observed at all developmental stages analyzed (from 10 to 100 days post fertilization, dpf), and the antibody used identified a protein with a molecular weight of 70kDa, at 100dpf (regarded to be adult). Cell expressing SK1 in adult animals were neurons of dorsal root and cranial nerve sensory ganglia, sympathetic neurons, sensory cells in neuromasts of the lateral line system and taste buds, crypt olfactory neurons and photoreceptors. Present results report for the first time the expression and the distribution of SK1 in the peripheral nervous system and sensory organs of adult zebrafish, and may contribute to set zebrafish as an interesting experimental model for calcium-activated potassium channels research. Moreover these findings are of potential interest because the potential role of SK as targets for the treatment of neurological diseases and sensory disorders.

  9. Wide variation in absolute cardiovascular risk assessment in Aboriginal and Torres Strait Islander people with Type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Bhakti Ramesh Vasant

    2016-03-01

    Full Text Available Background: Absolute cardiovascular risk assessment (CVRA is based on the combined effects of multiple risk factors and can identify asymptomatic individuals at high risk of cardiovascular disease. Aboriginal and Torres Strait Islander people are disproportionately affected by cardiovascular disease and diabetes. Our study aimed to investigate variations in the use of absolute CVRA in patients with diabetes at Indigenous community healthcare centres, and to identify patient and health centre characteristics that may contribute to this variation. Methods: Audits of clinical records of 1,728 patients with a known diagnosis of diabetes across 121 health centres over the period 2012–2014 were conducted as part of a large-scale continuous quality improvement program. Multilevel regression modelling was used to quantify variation in recording of CVRA attributable to health centre and patient characteristics. Results: The proportion of eligible patients with documented CVRA was 33% (n=574/1,728. The majority (95% of assessments were conducted in the Northern Territory (NT. Multilevel regression analysis showed health centre characteristics accounted for 70% of the variation in assessments in the NT. Government-operated health centres had 18.8 times the odds (95% CI 7.7–46.2 of recording CVRA delivery compared with other health centres. Conclusion: Health centres in the NT delivered the majority of absolute CVRA to Indigenous patients with diabetes in our study. Health systems factors that may have facilitated provision of CVRA in the NT include decision support tools and a reporting process for CVRA delivery. Implementation of similar systems in other jurisdictions may help improve CVRA delivery. Early identification and treatment of high risk individuals through wider use of CVRA may help reduce the burden of cardiovascular disease in Indigenous Australians with diabetes.

  10. Spatial clustering of toxic trace elements in adolescents around the Torreón, Mexico lead-zinc smelter.

    Science.gov (United States)

    Garcia-Vargas, Gonzalo G; Rothenberg, Stephen J; Silbergeld, Ellen K; Weaver, Virginia; Zamoiski, Rachel; Resnick, Carol; Rubio-Andrade, Marisela; Parsons, Patrick J; Steuerwald, Amy J; Navas-Acién, Ana; Guallar, Eliseo

    2014-11-01

    High blood lead (BPb) levels in children and elevated soil and dust arsenic, cadmium, and lead were previously found in Torreón, northern Mexico, host to the world's fourth largest lead-zinc metal smelter. The objectives of this study were to determine spatial distributions of adolescents with higher BPb and creatinine-corrected urine total arsenic, cadmium, molybdenum, thallium, and uranium around the smelter. Cross-sectional study of 512 male and female subjects 12-15 years of age was conducted. We measured BPb by graphite furnace atomic absorption spectrometry and urine trace elements by inductively coupled plasma-mass spectrometry, with dynamic reaction cell mode for arsenic. We constructed multiple regression models including sociodemographic variables and adjusted for subject residence spatial correlation with spatial lag or error terms. We applied local indicators of spatial association statistics to model residuals to identify hot spots of significant spatial clusters of subjects with higher trace elements. We found spatial clusters of subjects with elevated BPb (range 3.6-14.7 μg/dl) and urine cadmium (0.18-1.14 μg/g creatinine) adjacent to and downwind of the smelter and elevated urine thallium (0.28-0.93 μg/g creatinine) and uranium (0.07-0.13 μg/g creatinine) near ore transport routes, former waste, and industrial discharge sites. The conclusion derived from this study was that spatial clustering of adolescents with high BPb and urine cadmium adjacent to and downwind of the smelter and residual waste pile, areas identified over a decade ago with high lead and cadmium in soil and dust, suggests that past and/or present plant operations continue to present health risks to children in those neighborhoods.

  11. A mental health first aid training program for Australian Aboriginal and Torres Strait Islander peoples: description and initial evaluation

    Directory of Open Access Journals (Sweden)

    Hart Laura M

    2009-06-01

    Full Text Available Abstract Background Mental Health First Aid (MHFA training was developed in Australia to teach members of the public how to give initial help to someone developing a mental health problem or in a mental health crisis situation. However, this type of training requires adaptation for specific cultural groups in the community. This paper describes the adaptation of the program to create an Australian Aboriginal and Torres Strait Islander Mental Health First Aid (AMHFA course and presents an initial evaluation of its uptake and acceptability. Methods To evaluate the program, two types of data were collected: (1 quantitative data on uptake of the course (number of Instructors trained and courses subsequently run by these Instructors; (2 qualitative data on strengths, weaknesses and recommendations for the future derived from interviews with program staff and focus groups with Instructors and community participants. Results 199 Aboriginal people were trained as Instructors in a five day Instructor Training Course. With sufficient time following training, the majority of these Instructors subsequently ran 14-hour AMHFA courses for Aboriginal people in their community. Instructors were more likely to run courses if they had prior teaching experience and if there was post-course contact with one of the Trainers of Instructors. Analysis of qualitative data indicated that the Instructor Training Course and the AMHFA course are culturally appropriate, empowering for Aboriginal people, and provided information that was seen as highly relevant and important in assisting Aboriginal people with a mental illness. There were a number of recommendations for improvements. Conclusion The AMHFA program is culturally appropriate and acceptable to Aboriginal people. Further work is needed to refine the course and to evaluate its impact on help provided to Aboriginal people with mental health problems.

  12. A network approach to policy framing: A case study of the National Aboriginal and Torres Strait Islander Health Plan.

    Science.gov (United States)

    Browne, Jennifer; de Leeuw, Evelyne; Gleeson, Deborah; Adams, Karen; Atkinson, Petah; Hayes, Rick

    2017-01-01

    Aboriginal health policy in Australia represents a unique policy subsystem comprising a diverse network of Aboriginal-specific and "mainstream" organisations, often with competing interests. This paper describes the network structure of organisations attempting to influence national Aboriginal health policy and examines how the different subgroups within the network approached the policy discourse. Public submissions made as part of a policy development process for the National Aboriginal and Torres Strait Islander Health Plan were analysed using a novel combination of network analysis and qualitative framing analysis. Other organisational actors in the network in each submission were identified, and relationships between them determined; these were used to generate a network map depicting the ties between actors. A qualitative framing analysis was undertaken, using inductive coding of the policy discourses in the submissions. The frames were overlaid with the network map to identify the relationship between the structure of the network and the way in which organisations framed Aboriginal health problems. Aboriginal organisations were central to the network and strongly connected with each other. The network consisted of several densely connected subgroups, whose central nodes were closely connected to one another. Each subgroup deployed a particular policy frame, with a frame of "system dysfunction" also adopted by all but one subgroup. Analysis of submissions revealed that many of the stakeholders in Aboriginal health policy actors are connected to one another. These connections help to drive the policy discourse. The combination of network and framing analysis illuminates competing interests within a network, and can assist advocacy organisations to identify which network members are most influential. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Buena sangre y hábitos españoles: repensando a Alonso de Silva y Diego de Torres

    Directory of Open Access Journals (Sweden)

    Joanne Rappaport

    2012-05-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Este artículo explora la frontera porosa entre mestizos e indígenas en el Nuevo Reino de Granada durante el siglo XVI, interrogándose sobre cómo los mestizos aspirantes a los cacicazgos muiscas se presentaron a sí mismos y cómo fueron caracterizados por sus adversarios en la negociación de sus relaciones con la corona española, los encomenderos locales y sus propios vasallos. La reflexión se centrará, en particular, en dos hombres: don Alonso de Silva, cacique de Tibasosa, y don Diego de Torres, cacique de Turmequé; ambos hijos de mujeres pertenecientes a líneas de sucesión de los cacicazgos muiscas, pero que fueron criados en hogares paternos españoles. Las pretensiones de estos dos personajes sobre los cacicazgos contradecían las aspiraciones de otros mestizos de élite de su tiempo, quienes buscaban aceptación en la sociedad española de Santafé y Tunja. En contraste, los dos se describían sistemáticamente a sí mismos como candidatos  privilegiados para lograr el estatus de miembros de la élite indígena, recurriendo a marcadores de identidad cristianos e ibéricos.

  14. Development of a Humane Slaughter Device for Green Turtles for Use by Traditional Owners in the Torres Strait Islands, Australia.

    Science.gov (United States)

    Flint, Mark; Mills, Paul C; Loban, Frank; Simpson, Tristan; Lui, Stan; Fujii, Ronald; Whap, Don; Flint, Jaylene B; Owen, Helen

    2017-01-01

    Marine turtles are caught and slaughtered for consumption as part of traditional indigenous community harvest in Australia as well as in many countries in which marine turtles can be found. However, changes to the Animal Care and Protection Act 2001 in 2012 resulted in Australian indigenous hunters becoming potentially liable to prosecution for using traditional practices to slaughter marine turtles. To provide indigenous hunters with an alternative scientifically tested method to hunt, we developed and tested a humane method as an option to use in indigenous communities. Between 2012 and 2015, a device was developed, tested on 11 carcasses to determine effectiveness and repeatability, used on 5 anaesthetised animals independently diagnosed as candidates for euthanasia, and ultimately used on 2 healthy, conscious animals as part of normal indigenous community subsistence harvesting under observation before being left with the communities for use. Feedback was sought from the communities on the suitability and potential adoption of the device. The device effectively ablated the hind brain and severed the spinal cord when deployed in 81% (9/11) of the tested carcasses, with death in 100% (5/5) of turtles, on average, within 78 seconds of deployment on anaesthetised turtles and death in 100% (2/2) of turtles, on average, within 144 seconds when deployed on healthy turtles within community. Failure to ablate the hindbrain and sever the spinal cord in the cadaver cases was due to incorrect deployment of the device. This device showed promise as an alternative euthanasia method available to indigenous communities of the Torres Straits. Further work is required to encourage acceptance by hunters.

  15. Development of a Humane Slaughter Device for Green Turtles for Use by Traditional Owners in the Torres Strait Islands, Australia

    Science.gov (United States)

    Flint, Mark; Mills, Paul C.; Loban, Frank; Simpson, Tristan; Lui, Stan; Fujii, Ronald; Whap, Don; Flint, Jaylene B.; Owen, Helen

    2017-01-01

    Marine turtles are caught and slaughtered for consumption as part of traditional indigenous community harvest in Australia as well as in many countries in which marine turtles can be found. However, changes to the Animal Care and Protection Act 2001 in 2012 resulted in Australian indigenous hunters becoming potentially liable to prosecution for using traditional practices to slaughter marine turtles. To provide indigenous hunters with an alternative scientifically tested method to hunt, we developed and tested a humane method as an option to use in indigenous communities. Between 2012 and 2015, a device was developed, tested on 11 carcasses to determine effectiveness and repeatability, used on 5 anaesthetised animals independently diagnosed as candidates for euthanasia, and ultimately used on 2 healthy, conscious animals as part of normal indigenous community subsistence harvesting under observation before being left with the communities for use. Feedback was sought from the communities on the suitability and potential adoption of the device. The device effectively ablated the hind brain and severed the spinal cord when deployed in 81% (9/11) of the tested carcasses, with death in 100% (5/5) of turtles, on average, within 78 seconds of deployment on anaesthetised turtles and death in 100% (2/2) of turtles, on average, within 144 seconds when deployed on healthy turtles within community. Failure to ablate the hindbrain and sever the spinal cord in the cadaver cases was due to incorrect deployment of the device. This device showed promise as an alternative euthanasia method available to indigenous communities of the Torres Straits. Further work is required to encourage acceptance by hunters. PMID:28076432

  16. Spatial clustering of toxic trace elements in adolescents around the Torreón, Mexico lead–zinc smelter

    Science.gov (United States)

    Garcia-Vargas, Gonzalo G.; Rothenberg, Stephen J.; Silbergeld, Ellen K.; Weaver, Virginia; Zamoiski, Rachel; Resnick, Carol; Rubio-Andrade, Marisela; Parsons, Patrick J.; Steuerwald, Amy J.; Navas-Acién, Ana; Guallar, Eliseo

    2016-01-01

    High blood lead (BPb) levels in children and elevated soil and dust arsenic, cadmium, and lead were previously found in Torreón, northern Mexico, host to the world’s fourth largest lead–zinc metal smelter. The objectives of this study were to determine spatial distributions of adolescents with higher BPb and creatinine-corrected urine total arsenic, cadmium, molybdenum, thallium, and uranium around the smelter. Cross-sectional study of 512 male and female subjects 12–15 years of age was conducted. We measured BPb by graphite furnace atomic absorption spectrometry and urine trace elements by inductively coupled plasma-mass spectrometry, with dynamic reaction cell mode for arsenic. We constructed multiple regression models including sociodemographic variables and adjusted for subject residence spatial correlation with spatial lag or error terms. We applied local indicators of spatial association statistics to model residuals to identify hot spots of significant spatial clusters of subjects with higher trace elements. We found spatial clusters of subjects with elevated BPb (range 3.6–14.7 µg/dl) and urine cadmium (0.18–1.14 µg/g creatinine) adjacent to and downwind of the smelter and elevated urine thallium (0.28–0.93 µg/g creatinine) and uranium (0.07–0.13 µg/g creatinine) near ore transport routes, former waste, and industrial discharge sites. The conclusion derived from this study was that spatial clustering of adolescents with high BPb and urine cadmium adjacent to and downwind of the smelter and residual waste pile, areas identified over a decade ago with high lead and cadmium in soil and dust, suggests that past and/or present plant operations continue to present health risks to children in those neighborhoods. PMID:24549228

  17. A simple diabetes vascular severity staging instrument and its application to a Torres Strait Islander and Aboriginal adult cohort of north Australia

    Directory of Open Access Journals (Sweden)

    Gibson Odette R

    2012-07-01

    Full Text Available Abstract Background To develop an instrument that predicts diabetes-related vascular disease severity using routinely collected data on Australian Aboriginal and Torres Strait Islander adults with type 2 diabetes, in the absence of diabetes duration. Methods A complex diabetes severity classification system was simplified and adapted for use with an Australian Aboriginal and Torres Strait Islander adult population with type 2 diabetes in north Queensland. Detailed vascular health risks and morbidities were mapped to routinely collected measures. Individual–level health screening, hospital separation and mortality data were linked and used to plot mean monthly in-patient hospital cost and percent mortality by disease severity as defined by the newly developed instrument, to test construct validity. Results The revised instrument consists of four combined diabetes-related microvascular and macrovascular stages that range from least severe (stage 1 to severe irreversible vascular impairment (stage 4. When applied to data of an Aboriginal and Torres Strait Islander Australian population the instrument showed good construct validity, predicting higher hospital cost and mortality as vascular disease severity increased. Conclusions This instrument discriminates between levels of diabetes-related vascular disease severity, displays good construct validity by predicting increased hospital cost and mortality with worsening severity and can be populated with routinely collected data. It may assist with future health service research and its use could be extended to practice settings for health care planning for diabetes management programs and monitoring vascular disease progression.

  18. First report of a Trichinella papuae infection in a wild pig (Sus scrofa) from an Australian island in the Torres Strait region.

    Science.gov (United States)

    Cuttell, L; Cookson, B; Jackson, L A; Gray, C; Traub, R J

    2012-04-30

    Multiple Trichinella species are reported from the Australasian region although mainland Australia has never confirmed an indigenous case of Trichinella infection in humans or animals. Wildlife surveys in high-risk regions are essential to truly determine the presence or absence of Trichinella, but in mainland Australia are largely lacking. In this study, a survey was conducted in wild pigs from mainland Australia's Cape York Peninsula and Torres Strait region for the presence of Trichinella, given the proximity of a Trichinella papuae reservoir in nearby PNG. We report the detection of a Trichinella infection in a pig from an Australian island in the Torres Strait, a narrow waterway that separates the islands of New Guinea and continental Australia. The larvae were characterised as T. papuae (Kikori strain) by PCR and sequence analysis. No Trichinella parasites were found in any pigs from the Cape York Peninsula. These results highlight the link the Torres Strait may play in providing a passage for introduction of Trichinella parasites from the Australasian region to the Australian mainland.

  19. PAQR3增强乳腺癌细胞SK-BR-3的表柔比星敏感性%PAQR3 enhances chemosensitivity of breast cancer cell line SK-BR-3 to epirubicin

    Institute of Scientific and Technical Information of China (English)

    黄剑波; 罗鑫荣; 孔令泉; 向廷秀; 任国胜

    2013-01-01

    目的 探讨PAQR3增强乳腺癌细胞对表柔比星的敏感性.方法 采用Real-time PCR比较ER、PR及HER2受体不同的4株乳腺癌细胞(MDA-MB-231、MCF7、SK-BR-3及T47D)的PAQR3表达情况.在乳腺癌细胞SK-BR-3中转染人源性PAQR3全长表达质粒,设立对照组,观察比较转染PAQR3细胞组与空载质粒细胞组对表柔比星的敏感性,分别计算其所对应的IC50.通过细胞凋亡检测、Western blot探讨PAQR3增加表柔比星敏感性的机制.结果 在4株乳腺癌细胞中,受体三阴性细胞MDA-MB-231的PAQR3表达量最高,SK-BR-3与MCF7表达量相对较低;SK-BR-3过表达PAQR3后,其对表柔比星的化疗敏感性显著升高,IC50显著降低[(25.33±0.94) μg/mL vs(17.72±1.11) μg/mL,P<0.05];PAQR3本身并未促使SK-BR-3凋亡,实验组与对照组的凋亡率差异并无统计学意义(P>0.05).但在同等浓度表柔比星的作用下,PAQR3促使SK-BR-3表达凋亡相关蛋白Cleaved Caspase-7,从而导致表柔比星抑制率增加,对CleavedCaspase-3、Bcl-2、Bcl-xL表达则无影响.进一步分析发现,PAQR3可抑制乳腺癌化疗中表柔比星所致的ERK活化,因而促使乳腺癌细胞对表柔比星敏感.结论 PAQR3可提高乳腺癌细胞对表柔比星的化疗敏感性,PAQR3表达可能与乳腺癌对表柔比星的敏感性相关.

  20. Biological activity and binding of estradiol to SK-Mel 23 human melanoma cells

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    Sarti M.S.M.V.

    2004-01-01

    Full Text Available Patients expressing estradiol receptors in melanoma cells have been reported to have a better prognosis. We therefore decided to investigate the in vitro effects of ß-estradiol and tamoxifen on the growth and tyrosinase activity of SK-Mel 23 human melanoma cells. Twenty-four-hour treatment with 0.4 nM ß-estradiol inhibited cell proliferation in 30% (0.70 ± 0.03 x 10(5 cells and increased tyrosinase activity in 50% (7130.5 ± 376.5 cpm/10(5 cells, as compared to untreated cells (1.0 ± 0.05 x 10(5 cells and 4769 ± 25.5 cpm/10(5 cells, respectively. Both responses were completely (100% blocked by 1 µM tamoxifen. Higher concentrations (up to 1.6 nM or longer treatments (up to 72 h did not result in a larger effect of the hormone on proliferation or tyrosinase activity. Competition binding assays demonstrated the presence of binding sites to [2,4,6,7-³H]-ß-estradiol, and that the tritiated analogue was displaced by the unlabeled hormone (1 nM to 100 µM, Kd = 0.14 µM, maximal displacement of 93% or by 10 µM tamoxifen (displacement of 60%. ß-estradiol also increased the phosphorylated state of two proteins of 16 and 46 kDa, after 4-h treatment, as determined by Western blot. The absorbance of each band was 1.9- and 4-fold the controls, respectively, as determined with Image-Pro Plus software. Shorter incubation periods with ß-estradiol did not enhance phosporylation; after 6-h treatment with the hormone, the two proteins returned to the control phosphorylation levels. The growth inhibition promoted by estradiol may explain the better prognosis of melanoma-bearing women as compared to men, and open new perspectives for drug therapy.

  1. DJ1 Expression Downregulates in Neuroblastoma Cells (SK-N-MC Chronically Exposed to HIV-1 and Cocaine.

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    Upal eRoy

    2015-07-01

    Full Text Available Background: HIV-associated neurological disorder (HAND has long been recognized as a consequence of Human Immunodeficiency Virus (HIV infection in the brain. The pathology of HAND gets more complicated with the recreational drug use such as cocaine. Recent studies have suggested multiple genetic influences involved in the pathology of addiction and HAND but only a fraction of the entire genetic risk has been investigated so far. In this regard, role of DJ1 protein (a gene linked to autosomal recessive early-onset Parkinson’s disease in regulating dopamine transmission and reactive oxygen species (ROS production in neuronal cells will be worth investigating in HIV-1 and cocaine exposed microenvironment. Being a very abundant protein in the brain, DJ1 could serve as a potential marker for early detection of HIV-1 and/or cocaine related neurological disorder.Methods: In vitro analysis was done to observe the effect of HIV-1 and/or cocaine on DJ1 protein expression in neuroblastoma cells (SK-N-MC. Gene expression and protein analysis of DJ1 was done on the HIV infected and/or cocaine treated SK-N-MC and compared to untreated cells using real time PCR, Western Blot and flow cytometry.Results: Gene expression and protein analysis indicated that there was a significant decrease in DJ1 expression in SK-N-MC chronically exposed to HIV-1 and/or cocaine.Conclusion: This is the first study to establish that DJ1 expression level in the neuronal cells significantly decreased in presence of HIV-1and/or cocaine indicating oxidative stress level of dopamine neurons.

  2. La "Torre dei diamanti" in Castel Gavone. Un esempio di architettura del potere nel Finale alla fine del Quattrocento, a cura di Tiziano Mannoni e Giovanni Murialdo, Cd-rom, Comune di Finale Ligure, 2002

    OpenAIRE

    Paola Guglielmotti

    2003-01-01

    Review of La "Torre dei diamanti" in Castel Gavone. Un esempio di architettura del potere nel Finale alla fine del Quattrocento, a cura di Tiziano Mannoni e Giovanni Murialdo, Cd-rom, Comune di Finale Ligure, 2002 .

  3. La "Torre dei diamanti" in Castel Gavone. Un esempio di architettura del potere nel Finale alla fine del Quattrocento, a cura di Tiziano Mannoni e Giovanni Murialdo, Cd-rom, Comune di Finale Ligure, 2002

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    Paola Guglielmotti

    2003-06-01

    Full Text Available Review of La "Torre dei diamanti" in Castel Gavone. Un esempio di architettura del potere nel Finale alla fine del Quattrocento, a cura di Tiziano Mannoni e Giovanni Murialdo, Cd-rom, Comune di Finale Ligure, 2002 .

  4. Upregulation of SK3 and IK1 channels contributes to the enhanced endothelial calcium signaling and the preserved coronary relaxation in obese Zucker rats.

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    Belén Climent

    Full Text Available BACKGROUND AND AIMS: Endothelial small- and intermediate-conductance KCa channels, SK3 and IK1, are key mediators in the endothelium-derived hyperpolarization and relaxation of vascular smooth muscle and also in the modulation of endothelial Ca2+ signaling and nitric oxide (NO release. Obesity is associated with endothelial dysfunction and impaired relaxation, although how obesity influences endothelial SK3/IK1 function is unclear. Therefore we assessed whether the role of these channels in the coronary circulation is altered in obese animals. METHODS AND RESULTS: In coronary arteries mounted in microvascular myographs, selective blockade of SK3/IK1 channels unmasked an increased contribution of these channels to the ACh- and to the exogenous NO- induced relaxations in arteries of Obese Zucker Rats (OZR compared to Lean Zucker Rats (LZR. Relaxant responses induced by the SK3/IK1 channel activator NS309 were enhanced in OZR and NO- endothelium-dependent in LZR, whereas an additional endothelium-independent relaxant component was found in OZR. Fura2-AM fluorescence revealed a larger ACh-induced intracellular Ca2+ mobilization in the endothelium of coronary arteries from OZR, which was inhibited by blockade of SK3/IK1 channels in both LZR and OZR. Western blot analysis showed an increased expression of SK3/IK1 channels in coronary arteries of OZR and immunohistochemistry suggested that it takes place predominantly in the endothelial layer. CONCLUSIONS: Obesity may induce activation of adaptive vascular mechanisms to preserve the dilator function in coronary arteries. Increased function and expression of SK3/IK1 channels by influencing endothelial Ca2+ dynamics might contribute to the unaltered endothelium-dependent coronary relaxation in the early stages of obesity.

  5. Physical education, study of 3º the education in the towns of Torre de Moncorvo and Vila Nova de Gaia

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    Ruben Gonçalves Pereira

    2008-03-01

    Full Text Available The aim of this study that we proposed to develop was fundamentally guided as an attempt to answer a group of relevant questions: Is living in the interior of a country a restraining factor for the harmonious physical, mental and social development of the youth? Are physical aptitudes profiles distinct in terms of the level, depending on the fact that young people live in the interior or in the coast of a country? To answer these questions we develop a study in which we characterize and evaluate students of both sexes, from the 2nd and 3rd cycles of the Basic Education (from 5th to 9th grade, from schools of two diametrical opposed areas of Portugal: one school in a city by the coast - Vila Nova de Gaia, the other in the deep interior of Portugal - Torre de Moncorvo and we search the existence or not of an influence on the students' levels of physical aptitudes. This exploratory study was made from a random sample composed of 3271 subjects from the 5th grade to the 9th school grades. The battery of tests applied were the ones designated as "FACDEX" - Somato-Motor Development and Sports Excellence factors in the Portuguese Population. (Marques et al., 1991, obtaining a detailed analysis of the levels of Physical Aptitudes of young people from 10 to 17 years old. The results allowed us to conclude that the students of the Rural areas present a slightly higher standard in the Upper strength tests (2 kg ball, Medium strength (Sit Up's and Lower strength (Long jump. The students of the Urban Zone present higher standard levels in the Flexibility tests (Sit and Reach, Upper strength (Hockey ball Launching"; "Dinamometry"; quickness “10x5 Metres." and "Resistance 12". In terms of Speed, they show identical values. The students of the Urban Zone show higher weight values in terms of age in the female and male sex, with the exception of male individuals from the 14 to 16 years old and the girls after 15 years old. The girls of the Rural Environment

  6. Physical education, study of 3º the education in the towns of Torre de Moncorvo and Vila Nova de Gaia

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    R.G. Pereira

    2008-01-01

    Full Text Available The aim of this study that we proposed to develop was fundamentally guided as an attempt to answer a group of relevant questions: Is living in the interior of a country a restraining factor for the harmonious physical, mental and social development of the youth? Are physical aptitudes profiles distinct in terms of the level, depending on the fact that young people live in the interior or in the coast of a country? To answer these questions we develop a study in which we characterize and evaluate students of both sexes, from the 2nd and 3rd cycles of the Basic Education (from 5th to 9th grade, from schools of two diametrical opposed areas of Portugal: one school in a city by the coast - Vila Nova de Gaia, the other in the deep interior of Portugal- Torre de Moncorvo and we search the existence or not of an influence on the students’ levels of physical aptitudes. This exploratory study was made from a random sample composed of 3271 subjects from the 5th grade to the 9th school grades. The battery of tests applied were the ones designated as "FACDEX" - Somato-Motor Development and Sports Excellence factors in the Portuguese Population. (Marques et al., 1991, obtaining a detailed analysis of the levels of Physical Aptitudes of young people from 10 to 17 years old. The results allowed us to conclude tha the students of the Rural areas present a slightly higher standard in the Upper strength tests (2 kg ball , Medium strength (Sit Up' s and Lower strength (Long jump. The students of the Urban Zone present higher standard levels in the Flexibility tests (Sit and Reach, Upper strength (Hockey ball Launching"; "Dinamometry"; quickness “10x5 Metres ." and "Resistance 12'". In terms of Speed, they show identical values. The students of the Urban Zone show higher weight values in terms of age in the female and male sex, with the exception of male individuals from the 14 to 16 years old and the girls after 15 years old. The girls of the Rural Environment

  7. The Murri clinic: a comparative retrospective study of an antenatal clinic developed for Aboriginal and Torres Strait Islander women

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    Kildea Sue

    2012-12-01

    Full Text Available Abstract Background Indigenous Australians are a small, widely dispersed population. Regarding childbearing women and infants, inequities in service delivery and culturally unsafe services contribute to significantly poorer outcomes, with a lack of high-level research to guide service redesign. This paper reports on an Evaluation of a specialist (Murri antenatal clinic for Australian Aboriginal and Torres Strait Islander women. Methods A triangulated mixed method approach generated and analysed data from a range of sources: individual and focus group interviews; surveys; mother and infant audit data; and routinely collected data. A retrospective analysis compared clinical outcomes of women who attended the Murri clinic (n=367 with Indigenous women attending standard care (n=414 provided by the same hospital over the same period. Both services see women of all risk status. Results The majority of women attending the Murri clinic reported high levels of satisfaction, specifically with continuity of carer antenatally. However, disappointment with the lack of continuity during labour/birth and postnatally left some women feeling abandoned and uncared for. Compared to Indigenous women attending standard care, those attending the Murri clinic were statistically less likely to be primiparous or partnered, to experience perineal trauma, to have an epidural and to have a baby admitted to the Neonatal Intensive Care Unit, and were more likely to have a non-instrumental vaginal birth. Multivariate analysis found higher normal birth (spontaneous onset of labour, no epidural, non-instrumental vaginal birth without episiotomy rates amongst women attending the Murri clinic. Conclusions Significant benefits were associated with attending the Murri clinic. Recommendations for improvement included ongoing cultural competency training for all hospital staff, reducing duplication of services, improving co-ordination and communication between community and tertiary

  8. Prevalence of Hepatitis C Among Australian Aboriginal and Torres Strait Islander people: A Systematic Review and Meta-Analysis

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    Graham

    2016-07-01

    Full Text Available Context Aboriginal and Torres Strait Islanders (Aboriginal account for approximately 3% of the Australian population. They have the poorest health, economic and social outcomes. Higher notification rates of hepatitis C antibodies (anti-HCV have been reported among Aboriginal compared with non-Aboriginal people. The identification of Aboriginal people in national surveillance has some weaknesses, with only four of the eight jurisdictions included in national reporting. To address some of these limitations, we aim to estimate the pooled prevalence of anti-HCV among Aboriginal people in Australia. Evidence Acquisition We searched the databases: Pubmed, Web of Science and Informit, and the New South Wales and Northern Territory Public Health Bulletins. A study was included if it reported the number of Aboriginal people testing positive for anti-HCV and the number tested for anti-HCV. A meta-analysis by population-group was conducted if three or more studies reported a prevalence estimate. Variables included: author, year of publication, study design, study period, gender (female, male, age, population group (Aboriginal people in prison, Aboriginal people who inject drugs, number testing anti-HCV positive, number tested for anti-HCV and prevalence (%. Due to a long time period, we separated the studies estimating the prevalence anti-HCV among Aboriginal people in prison into two time periods, 1994 - 2004 and 2005 - 2012. Results Overall, 15 studies met our inclusion criteria. Among Aboriginal people in prison, the pooled prevalence of anti-HCV was 18.1% (95%CI: 6.6 - 29.7. The pooled prevalence among Aboriginal people in prison was 25.7% (95%CI: 4.1-47.3 in studies published between 1994 - 2004 and 14.5% (95%CI: 1.7 - 27.3 in studies published from 2005 - 2012. The pooled prevalence of anti-HCV was 58.7% (95%CI: 53.9 - 63.5 among Aboriginal people who inject drugs and 2.9% (95%CI: 0.30 - 6.1 among Aboriginal people who did not inject drugs, however

  9. Social and Emotional Wellbeing Screening for Aboriginal and Torres Strait Islanders within Primary Health Care: A Series of Missed Opportunities?

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    Erika Langham

    2017-07-01

    Full Text Available BackgroundSocial and emotional wellbeing (SEWB is a critical determinant of health outcomes for Indigenous Australians. This study examined the extent to which primary healthcare services (PHSs undertake SEWB screening and management of Aboriginal and Torres Strait Islander clients, and the variation in SEWB screening and management across Indigenous PHS.MethodsCross-sectional analysis between 2012 and 2014 of 3,407 Indigenous client records from a non-representative sample of 100 PHSs in 4 Australian states/territory was undertaken to examine variation in the documentation of: (1 SEWB screening using identified measurement instruments, (2 concern regarding SEWB, (3 actions in response to concern, and (4 follow up actions. Binary logistic regression was used to determine the factors associated with screening.ResultsThe largest variation in SEWB screening occurred at the state/territory level. The mean rate of screening across the sample was 26.6%, ranging from 13.7 to 37.1%. Variation was also related to PHS characteristics. A mean prevalence of identified SEWB concern was 13% across the sample, ranging from 9 to 45.1%. For the clients where SEWB concern was noted, 25.4% had no referral or PHS action recorded. Subsequent internal PHS follow up after 1 month occurred in 54.7% of cases; and six-monthly follow up of referrals to external services occurred in 50.9% of cases.ConclusionOur findings suggest that the lack of a clear model or set of guidelines on best practice for screening for SEWB in Indigenous health may contribute to the wide variation in SEWB service provision. The results tell a story of missed opportunities: 73.4% of clients were not screened and no further action was taken for 25.4% for whom an SEWB concern was identified. There was no follow up for just under half of those for whom action was taken. There is a need for the development of national best practice guidelines for SEWB screening and management, accompanied by

  10. P2X(7) receptor activation enhances SK3 channels- and cystein cathepsin-dependent cancer cells invasiveness.

    Science.gov (United States)

    Jelassi, B; Chantôme, A; Alcaraz-Pérez, F; Baroja-Mazo, A; Cayuela, M L; Pelegrin, P; Surprenant, A; Roger, S

    2011-05-05

    ATP-gated P2X(7) receptors (P2X(7)R) are unusual plasma membrane ion channels that have been extensively studied in immune cells. More recently, P2X(7)R have been described as potential cancer cell biomarkers. However, mechanistic links between P2X(7)R and cancer cell processes are unknown. Here, we show, in the highly aggressive human breast cancer cell line MDA-MB-435s, that P2X(7) receptor is highly expressed and fully functional. Its activation is responsible for the extension of neurite-like cellular prolongations, of the increase in cell migration by 35% and in cell invasion through extracellular matrix by 150%. The change in cancer cell morphology and the increased migration appeared to be due to the activation of Ca(2+)-activated SK3 potassium channels. The enhanced invasion through the extracellular matrix was related to the increase of mature forms of cysteine cathepsins in the extracellular medium, which was independent of SK3 channel activity and not associated with cell death. Pharmacological targeting of P2X(7)R in vivo was crucial for cell invasiveness in a zebrafish model of metastases. Our results demonstrate a novel mechanistic link between P2X(7)R functionality in cancer cells and invasiveness, a key parameter in tumour growth and in the development of metastases. They also suggest a potential therapeutic role for the newly developed P2X(7)R antagonists.

  11. The Effects of Boswellia Resin Extract on Dopaminergic Cell line, SK-N-SH, against MPP+-Induced Neurotoxicity

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    Sareh Kazmi

    2011-10-01

    Full Text Available Introduction: Oxidative stress and neuroinflammation are involved in neurodegeneration procedure in Parkinson’s disease. Recently, neuroprotective potential of Boswellia resin has been demonstrated. Therefore, this study examined whether administration of Boswellia resin would attenuate MPP+- induced neuronal death in SK-N-SH- cell line, a human dopaminergic neurons- in vitro. Methods: Boswellia resin extract was added to culture medium (10μg/ml before and after exposure of SK-N-SH cell line to MPP+ (1000μM. Cell viability and apoptosis features were assessed using MTT and Hoechst staining, respectively. Results: Treatment with Boswellia resin 2 and 3h prior to MPP ° exposure and up to 60 minutes after MPP ° exposure significantly increased cell viability compare to untreated cells. Apoptotic features were also reduced significantly by Boswellia resin (10 μg/ml compare to that of control untreated cells. Discussion: Boswellia resin has neuroprotective effects on dopaminergic neurons which can be applicable in Parkinson’s disease.

  12. Spectral Knowledge (SK-UTALCA): Software for Exploratory Analysis of High-Resolution Spectral Reflectance Data on Plant Breeding

    Science.gov (United States)

    Lobos, Gustavo A.; Poblete-Echeverría, Carlos

    2017-01-01

    This article describes public, free software that provides efficient exploratory analysis of high-resolution spectral reflectance data. Spectral reflectance data can suffer from problems such as poor signal to noise ratios in various wavebands or invalid measurements due to changes in incoming solar radiation or operator fatigue leading to poor orientation of sensors. Thus, exploratory data analysis is essential to identify appropriate data for further analyses. This software overcomes the problem that analysis tools such as Excel are cumbersome to use for the high number of wavelengths and samples typically acquired in these studies. The software, Spectral Knowledge (SK-UTALCA), was initially developed for plant breeding, but it is also suitable for other studies such as precision agriculture, crop protection, ecophysiology plant nutrition, and soil fertility. Various spectral reflectance indices (SRIs) are often used to relate crop characteristics to spectral data and the software is loaded with 255 SRIs which can be applied quickly to the data. This article describes the architecture and functions of SK-UTALCA and the features of the data that led to the development of each of its modules. PMID:28119705

  13. Sedimentation from suspension and sediment accumulation rate in the River Vistula prodelta, Gulf of Gdańsk (Baltic Sea

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    Mateusz Damrat

    2013-11-01

    Full Text Available The River Vistula is one of the largest suppliers of fresh water and terrigenous matter to the Baltic Sea. The impact of this river on the Baltic coastal system and the fate of the sediment delivered to the Gulf of Gdańsk are not well understood. Spatial transport patterns, as well as the settling, deposition and accumulation of the sediments were studied at the Vistula prodelta in different seasons from January 2012 to January 2013. The concentration of suspended matter in the water column was measured with optical methods, the sedimentation rate was determined with sediment traps, and the sediment accumulation rate was estimated using 210Pb dating. Our data shows that the annual supply of sediment to the sediment-water interface exceeds the annual rate of sediment accumulation in the outer Vistula prodelta by a factor of three. Sediment trapping during rough weather showed that significant sediment redeposition was taking place, even at depths of 55 m. The dynamic sedimentary processes occurring in the Vistula prodelta mean that that more than two-thirds of the sediment mass can be remobilized and then redeposited in deeper parts of the Gdańsk Basin.

  14. Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.

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    Giuliana Napolitano

    Full Text Available BACKGROUND: The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb from the large inactive multisubunit 7SK snRNP complex. P-TEFb is required for transcription of most class II genes through stimulation of RNA polymerase II elongation and cotranscriptional pre-mRNA processing. METHODOLOGY/PRINCIPAL FINDINGS: We show here that caffeine prevents UV-induced dissociation of P-TEFb as well as transcription inhibition. The caffeine-effect does not involve PI3-kinase-related protein kinases, because inhibition of phosphatidylinositol 3-kinase family members (ATM, ATR and DNA-PK neither prevents P-TEFb dissociation nor transcription inhibition. Finally, caffeine prevention of transcription inhibition is independent from DNA damage. CONCLUSION/SIGNIFICANCE: Pharmacological prevention of P-TEFb/7SK snRNP dissociation and transcription inhibition following UV-induced DNA damage is correlated.

  15. In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2(T) hydrocarbon biodegradation.

    Science.gov (United States)

    Matturro, Bruna; Frascadore, Emanuela; Cappello, Simone; Genovese, Mariella; Rossetti, Simona

    2016-09-15

    This study aimed to develop a new assay based on the whole cell hybridization in order to monitor alkane hydroxylase genes (alkB system) of the marine bacterium Alcanivorax borkumensis SK2(T) commonly reported as the predominant microorganism responsible for the biodegradation of n-alkanes which are the major fraction of petroleum hydrocarbons. The assay based on the whole cell hybridization targeting alkB2 gene was successfully developed and calibrated on a pure culture of Alcanivorax borkumensis SK2(T) with a detection efficiency up to 80%. The approach was further successfully validated on hydrocarbon-contaminated seawater and provided cells abundance (6.74E+04alkB2-carryingcellsmL(-1)) higher of about one order of magnitude than those obtained by qPCR (4.96E+03alkB2genecopiesmL(-1)). This study highlights the validity of the assay for the detection at single cell level of key-functional genes involved in the biodegradation of n-alkanes.

  16. EFEKTIVITAS PERSYARATAN HYGIENE SANITASI TERHADAP USAHA RUMAH MAKAN DAN RESTORAN BERDASARKAN KEPMENKES RI NOMOR 1098/MENKES/SK/VII/2003

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    Gusti Ayu Dita Nomia Sari

    2016-09-01

    Full Text Available Tourists who visited tourism destination in addition to the cultural and natural environment, food and drinks being the main attraction. Lack of awareness of the restaurant business to maintain cleanliness and sanitation are not uncommon cause of food poisoning. Legal issues raised in this study are: a How obligation of entrepreneur restaurants and legal protection for consumers under the Consumer Protection Act; b how the effectiveness of sanitation hygiene is based on Kepmenkes No. 1098 / Menkes / SK / VII / 2003 against the restaurants in the Ubud area. This study uses empirical legal research . The data is processed by analyzing qualitative descriptive analytical. Wisatawan yang mengunjungi destinasi pariwisata selain budaya dan keindahan alam, makanan dan minuman menjadi daya tarik utama lainnya. Kurangnya kesadaran pengusaha restoran untuk menjaga kebersihan dan sanitasi yang tidak jarang menyebabkan terjadinya keracunan makanan. Isu hukum yang diangkat dalam penelitian ini adalah: a Bagaimana kewajiban pelaku usaha rumah makan dan restoran dan perlindungan hukum bagi konsumen menurut UU Perlindungan Konsumen; b bagaimana efektivitas hygiene sanitasi restoran berdasarkan Kepmenkes No. 1098 / Menkes / SK / VII / 2003 terhadap rumah makan dan restoran di kawasan ubud. Penelitian ini menggunakan penelitian hukum empiris . Data diolah dengan menganalisis deskriptif kualitatif analitis.

  17. “El libro que contiene onze partidos del M. Dn Joseph de Torres”. ¿Torres y Martínez Bravo, o Torres y Vergara? Aproximaciones a una interpretación filológica del manuscrito de la colección Sánchez Garza

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    Delgado Parra, Gustavo

    2008-12-01

    Full Text Available The Torres Book from the Sanchez Garza collection is one of the most polemic issues in the study of Hispanic music. This controversy has been the result of two main factors. On the one hand, the authorship problems and on the other, the apparent disorder of the musical content in this manuscript due to the ineffi cient work done by the copiers who gave it form. The latter has lead to conclude, as a generalized opinion, that it is a highly corrupted copy of the organ works that could have been composed by the famous Spanish composer, organist, musical theorist and printer, Joseph de Torres y Martinez Bravo (*1670c; †1738; in which case the copier would be the one to blame for the demerit of such a renowned musician of the Royal Chapel of Madrid. If to this we add the transcription offered by the CENIDIM in 1993 which multiplies the confusion, we are facing pieces of music full of questions and a musical text which is neither effective nor convincing.El Libro de Torres de la colección Sánchez Garza, es uno de los documentos más polémicos de los últimos tiempos en la musicología hispánica. Esta polémica ha sido el resultado de dos factores fundamentales: por un lado el problema de la autoría, y por el otro, el aparente desorden del contenido musical del manuscrito, debido supuestamente, al trabajo inefi ciente de los copistas que le dieron forma. Esto último ha llevado a concluir, como opinión generalizada, que se trata de una copia corrompida de las obras para órgano que habrían sido compuestas por el prestigiado compositor, organista, teórico musical e impresor español, Joseph de Torres y Martínez Bravo (*1670c; †1738, en cuyo caso el copista del manuscrito sería el responsable de dicho desorden; lo que ha llevado a suponer, a otros investigadores, que incluso las obras en cuestión no sean de su autoría. A esto debemos agregar la confusa y a veces errónea transcripción ofrecida por el CENIDIM en 1993, en cuyo caso las

  18. In vitro detection of human breast cancer cells (SK-BR3) using herceptin-conjugated liquid crystal microdroplets as a sensing platform.

    Science.gov (United States)

    Ding, Wang; Gupta, Kailash Chandra; Park, Soo-Young; Kim, Young-Kyoo; Kang, Inn-Kyu

    2016-10-20

    The present study utilizes antibody-protein interactions to develop an LC microdroplet based biosensor for naked eye detection of SK-BR3 human breast cancer cells. The herceptin antibody-conjugated LC microdroplets were fabricated using 4-cyano-4'-pentyl biphenyl (5CB) as the liquid crystalline phase and sodium dodecyl sulfate (SDS) as the surfactant. The poly (styrene-b-acrylic acid) amphiphilic block copolymer (PS-b-PA) played a role as a modifier for the liquid crystalline interfaces. The 5CB molecules in the herceptin antibody-conjugated LC microdroplets have shown an orientation transition from radial to bipolar on selective interactions with targeted SK-BR3 breast cancer cells, which are over expressed by the human epidermal growth factor receptor 2-positive (HER2). The herceptin antibody-conjugated LC microdroplets are found to be highly selective in the detection of SK-BR3 cancer cells in the presence of control cells, such as KB cancer cells and fibroblast (FB), and also in the presence of 10% human blood plasma. The interaction forces of the SK-BR3 cancer cells were only effective in causing orientation transitions in 5CB molecules in the LC microdroplets, which clearly suggested that the herceptin antibody-conjugated LC microdroplets could be used as a selective biosensor for a real-time detection of SK-BR3 cancer cells in biological fluids.

  19. Transcription Factors Mediate the Enzymatic Disassembly of Promoter-Bound 7SK snRNP to Locally Recruit P-TEFb for Transcription Elongation

    Directory of Open Access Journals (Sweden)

    Ryan P. McNamara

    2013-12-01

    Full Text Available The transition from transcription initiation into elongation is controlled by transcription factors, which recruit positive transcription elongation factor b (P-TEFb to promoters to phosphorylate RNA polymerase II. A fraction of P-TEFb is recruited as part of the inhibitory 7SK small nuclear ribonucleoprotein particle (snRNP, which inactivates the kinase and prevents elongation. However, it is unclear how P-TEFb is captured from the promoter-bound 7SK snRNP to activate elongation. Here, we describe a mechanism by which transcription factors mediate the enzymatic release of P-TEFb from the 7SK snRNP at promoters to trigger activation in a gene-specific manner. We demonstrate that Tat recruits PPM1G/PP2Cγ to locally disassemble P-TEFb from the 7SK snRNP at the HIV promoter via dephosphorylation of the kinase T loop. Similar to Tat, nuclear factor (NF-κB recruits PPM1G in a stimulus-dependent manner to activate elongation at inflammatory-responsive genes. Recruitment of PPM1G to promoter-assembled 7SK snRNP provides a paradigm for rapid gene activation through transcriptional pause release.

  20. Cross-sector collaborations in Aboriginal and Torres Strait Islander childhood disability: a systematic integrative review and theory-based synthesis.

    Science.gov (United States)

    Green, Anna; DiGiacomo, Michelle; Luckett, Tim; Abbott, Penelope; Davidson, Patricia Mary; Delaney, Joanne; Delaney, Patricia

    2014-12-18

    Aboriginal and Torres Strait Islander children in Australia experience a higher prevalence of disability and socio-economic disadvantage than other Australian children. Early intervention is vital for improved health outcomes, but complex and fragmented service provision impedes access. There have been international and national policy shifts towards inter-sector collaborative responses to disability, but more needs to be known about how collaboration works in practice. A systematic integrative literature review using a narrative synthesis of peer-reviewed and grey literature was undertaken to describe components of inter- and intra-sector collaborations among services to Aboriginal and Torres Strait Islander children with a disability and their families. The findings were synthesized using the conceptual model of the ecological framework. Thirteen articles published in a peer-reviewed journal and 18 articles from the grey literature met inclusion criteria. Important factors in inter- and intra-sector collaborations identified included: structure of government departments and agencies, and policies at the macro- (government) system level; communication, financial and human resources, and service delivery setting at the exo- (organizational) system level; and relationships and inter- and intra-professional learning at the meso- (provider) system level. The policy shift towards inter-sector collaborative approaches represents an opportunity for the health, education and social service sectors and their providers to work collaboratively in innovative ways to improve service access for Aboriginal and Torres Strait Islander children with a disability and their families. The findings of this review depict a national snapshot of collaboration, but as each community is unique, further research into collaboration within local contexts is required to ensure collaborative solutions to improve service access are responsive to local needs and sustainable.

  1. Entre experiência e invenção: incidências autobiográficas em Antônio Torres

    Directory of Open Access Journals (Sweden)

    Rogério Gustavo Gonçalves

    2016-01-01

    Full Text Available En la literatura de Antonio Torres, la presencia de elementos que hacen referencia a las experiencias personales y a las características específicas de su vida, elaborados artísticamente, hace que el memorialismo, que gobierna la narración de la historia d e los personajes, mézclese, a veces, con recuerdos íntimos del escritor. Basando - se en estas características, este artículo pretende estudiar, en las novelas de Antonio Torres, los procedimientos de ficcionalización de la experiencia, dada su importancia e n el proceso de construcción narrativa y en la relación autor - texto - lector. De la lectura dialógica de la totalidad de su obra, buscamos comprender la delineación de un "mito del escritor" o de una personalidad literaria y la consiguiente construcción de u n espacio autobiográfico, que se traduce en un universo ficticio singular donde personajes, temas y situaciones reaparecen con frecuencia, produciendo un enlace comunicativo entre los textos y causando la familiaridad inmediata del lector. Además de la pro ducción de una imagen literaria de Antonio Torres, nos fijamos en cómo la migración de elementos personales a su obra permite la entrada de información de la realidad socio - política de que es testigo en la esfera ficcional. Aprovechando su tierra y su pueb lo para retratar el impacto del cambio social en la gente del sertão del noreste de Brasil, la obra del autor termina expresando la preocupación de mostrar la crisis de identidad de estos sujetos del noreste, unida a los recuerdos de un pasado que no se re concilia al momento actual.

  2. Working at the interface in Aboriginal and Torres Strait Islander health: focussing on the individual health professional and their organisation as a means to address health equity.

    Science.gov (United States)

    Wilson, Annabelle M; Kelly, Janet; Magarey, Anthea; Jones, Michelle; Mackean, Tamara

    2016-11-17

    Aboriginal and Torres Strait Islander people experience inequity in health outcomes in Australia. Health care interactions are an important starting place to seek to address this inequity. The majority of health professionals in Australia do not identify as Aboriginal and/or Torres Strait Islander people and the health care interaction therefore becomes an example of working in an intercultural space (or interface). It is therefore critical to consider how health professionals may maximise the positive impact within the health care interaction by skilfully working at the interface. Thirty-five health professionals working in South Australia were interviewed about their experiences working with Aboriginal people. Recruitment was through purposive sampling. The research was guided by the National Health and Medical Research Council Values and Ethics for undertaking research with Aboriginal communities. Critical social research was used to analyse data. Interviews revealed two main types of factors influencing the experience of non-Aboriginal health professionals working with Aboriginal people at the interface: the organisation and the individual. Within these two factors, a number of sub-factors were found to be important including organisational culture, organisational support, accessibility of health services and responding to expectations of the wider health system (organisation) and personal ideology and awareness of colonisation (individual). A health professional's practice at the interface cannot be considered in isolation from individual and organisational contexts. It is critical to consider how the organisational and individual factors identified in this research will be addressed in health professional training and practice, in order to maximise the ability of health professionals to work with Aboriginal and Torres Strait Islander people and therefore contribute to addressing health equity.

  3. Epibionts and parasites on crustaceans (Copepoda, Cladocera, Cirripedia larvae inhabiting the Gulf of Gdańsk (Baltic Sea in very large numbers* This work was supported in part by grant No. BW/1320-5-0183-3 from the University of Gdańsk.

    Directory of Open Access Journals (Sweden)

    Luiza Bielecka

    2014-06-01

    Infestation by epibionts and parasites was not restricted to calanoid copepods: it was also detected in non-negligible numbers on other crustaceans, namely, Harpacticoida, Cladocera (Bosmina sp. and Cirripedia larvae (nauplii in the Gulf of Gdańsk.

  4. Study on meter-scale cycles of the Lower Cretaceous Quantou Formation in SK-1 well%SK-1井下白垩统泉头组米级旋回研究

    Institute of Scientific and Technical Information of China (English)

    郭娟娟; 吴欣松; 潘建国; 魏东涛; 付建伟

    2012-01-01

    Based on the data of core and well logging of SK-1 well,this paper carried out the sedimentary microfacies identification,and divided cycle into third-order,fourth-order,fifth-order and meter-scale cycles.According to the cyclic sequence,this paper carried out the Fischer graphic analysis,and established cyclic stratigraphic framework.The absolute age ofQuantou Formation in SongliaoBasin was obtained by usingwell logging spectral analysis,and the cycle times were estimated.The result shows that the fifth-order cycle is 112 ka and fourth-order cycle is 379 ka,which is separately corresponding toMilankovitch eccentricity short circle 85~140 ka and Milankovitch eccentricity long circle 350~400 ka.The results coincide with the result of logging.This further illustrates that the forming of cycle sequence formation is controlled byMilankovitch cycle,which provides scientific foundation for the test of influencing factors of climate change in Cretaceous period.%通过对SK-1井泉头组岩心的详细观察与描述,并结合测井曲线特征,开展了泉头组沉积微相的识别,并在此基础上,划分了Ⅲ级、Ⅳ级、Ⅴ级及米级旋回。通过对所划分的旋回地层进行Fischer图解分析,建立了反映泉头组地层叠置关系的旋回地层格架。利用测井谱分析,结合前人分析测试得到的松辽盆地泉头组的绝对年龄,对各级旋回进行周期计算,发现Ⅴ级旋回周期约为112ka,Ⅳ级旋回周期约为379ka,分别与米兰科维奇偏心率短周期85~140ka和长周期350~400ka相对应,从而表明SK-1井旋回地层的形成受米兰科维奇旋回的控制。此研究结果为早白垩世气候变化对旋回地层的影响研究提供了科学依据。

  5. Estudio de las torres de refrigeración asociadas a brotes comunitarios de legionelosis Study of refrigeration towers associated with community outbreaks of legionellosis

    Directory of Open Access Journals (Sweden)

    Albert Pérez Lleonart

    2007-08-01

    Full Text Available Objetivo: Evaluar los factores de riesgo de las torres de refrigeración asociadas a un brote comunitario de legionelosis según sus características de mantenimiento y la calidad del agua. Método: Comparar los datos recogidos entre los 4 tipos de torres de refrigeración: control 1, control 2, probable y confirmada. Se han estudiado un total de 184 torres de refrigeración asociadas a 17 brotes comunitarios de legionelosis de la provincia de Barcelona durante el año 2004, de las cuales 112 son control 1, 54 control 2, 8 probables y 10 confirmadas. Resultados: Las torres confirmadas se caracterizan por: niveles elevados de conductividad, aerobios totales, dureza cálcica, sólidos totales en disolución, temperatura y turbidez; concentraciones de cloro deficientes (Objetive: To evaluate the factors of risk of the risk cooling towers associated to a community outbreak of legionellosis according to its characteristics of maintenance and the quality of the water with the purpose of improving the systems of prevention of this one disease. Method: To compare the piece of information gathered between the 4 types of cooling towers: control 1, control 2, probable and confirmed. A total of 184 cooling towers have been studied associated to 17 community outbreak of legionellosis of the province of Barcelona (Spain during the year 2004, of which 112 are control 1, 54 control 2, 8 probable and 10 confirmed. Results: The confirmed towers are characterized by: high levels of conductivity, total aerobes, calcic hardness, total solids in dissolution, temperature and turbidity; low levels of chlorine (< 2 ppm; of use hypochlorite as disinfectant in a 10%; greater degree of breach of the revision program and cleaning of the internal elements of the tower (tray, stuffed and separating of drops; degree of performance of the periodic disinfections and the cleaning of deposits of the 100%. A 47% of the investigated towers do not use the previous treatments of the

  6. Del cristianismo a la teosofía : “Père Soleil” de Joaquín Torres-García

    OpenAIRE

    Vidal, Edgard

    2014-01-01

    International audience; Resumen : Este artículo se propone estudiar las interrelaciones entre la obra constructiva y el pensamiento esotérico en la obra de Joaquín Torres-García, concentrándose sobre un momento importante en la vida del pintor, el libro " Père Soleil " , escrito en Paris y firmado por el autor en 1931. A partir de una base de datos lexicales de los términos con letras mayúsculas, son descubiertas numerosas correlaciones de este texto con el corpus teosófico francés de fines d...

  7. Proposta de ferramenta para o gerenciamento do risco operacional nas operações realizadas pela torre de controle no Aeroporto Internacional de São Paulo - Guarulhos

    OpenAIRE

    Erik Augusto Geraldis

    2012-01-01

    Os profissionais do serviço de tráfego aéreo usualmente trabalham prestando um serviço ininterrupto composto pelas mais diversas composições de eventos, o que eventualmente pode proporcionar riscos aos usuários e comunidade aeronáutica. A segurança neste serviço depende, dentre outros fatores, da capacidade de identificar perigos e do gerenciamento dos riscos inerentes à atividade. Com o objetivo de identificar perigos no serviço de controle de tráfego aéreo prestado pela Torre de Controle do...

  8. Diseño de receptor de torre central para generación directa de vapor con perfil de potencia variable longitudinal y transversalmente

    OpenAIRE

    Losada Godoy, Carlos

    2014-01-01

    En este estudio se ha realizado el diseño de un receptor de una central de Torre Central de energía solar para generación directa de vapor, mediante el uso de métodos numéricos, con un perfil de potencia incidente variable longitudinal y transversalmente. Para ello se ha dividido la geometría del receptor según el método de diferencias finitas, y se ha procedido a resolver las ecuaciones del balance de energía. Una vez resuelto el sistema de ecuaciones se dispone de la distribución de tempe...

  9. La contribución de Manuel de Torres a la economía agraria en España (1930-1960)

    OpenAIRE

    Zabalza, Juan

    2004-01-01

    Este trabajo analiza, desde una perspectiva de historia del pensamiento económico, la introducción del análisis económico en el estudio de la agricultura española a través de los escritos agrarios de Manuel de Torres durante el periodo 1930-1960. Se ha incidido en el modelo analítico que subyace a sus trabajos sobre la agricultura española y en la correspondencia entre la evolución de su pensamiento económico y su análisis agrario a lo largo del periodo. Finalmente, el trabajo ha contribuido ...

  10. Study protocol: a pragmatic randomised controlled trial of a 12-week physical activity and nutritional education program for overweight Aboriginal and Torres Strait Islander women

    Directory of Open Access Journals (Sweden)

    Cargo Margaret

    2011-08-01

    Full Text Available Abstract Background Aboriginal and Torres Strait Islander women have a higher prevalence and incidence of obesity and type 2 diabetes than non-Indigenous Australian women. Physical inactivity is a key modifiable risk factor for obesity and evidence shows that even modest reductions in waist circumference (WC have significant health benefits. Trialing physical activity programs in difficult-to-reach high risk groups, especially urban Indigenous Australians poses distinct implementation challenges. Methods/Design The trial objective is to evaluate the effectiveness of a structured 12-week physical activity group program with nutritional advice. The design is a pragmatic randomised controlled trial. This study protocol describes the implementation and evaluation of the program. Participants are randomised into either an intervention or waitlisted group. The waitlisted group have a 12 month waiting period before commencing the 12-week program. Participant data is collected at baseline, 12, 24 and 52 weeks. Participants are Aboriginal and Torres Strait Islander women, aged 18-64 years with a waist circumference greater than 80 centimetres residing in Adelaide. The primary outcome measure is WC change immediately post program from baseline. Secondary outcomes include short term and long term changes in WC, weight, blood pressure, fasting blood glucose, insulin, insulin resistance (calculated HOMA, haemoglobin A1C (HbA1C, triglycerides and C-reactive protein (CRP. Behavioural and psychosocial surveys are administered to assess physical activity, dietary intake and the participant's motivation, self-efficacy and perceived social support for physical activity. Qualitative interviews focusing on participants' motivation, enablers and barriers to healthy eating and physical activity will be undertaken. Implementation fidelity and participation are also assessed. Discussion The Aboriginal and Torres Strait Islander Women's Fitness Program (WFP is designed

  11. Diseño y construcción de banco de pruebas para torres de transmisión eléctrica

    OpenAIRE

    Toledo Torres, Enrique Roman; Falconi, Wiesner

    2009-01-01

    Se procedió a la construcción de un banco de pruebas a escala natural para torres auto soportadas usadas en las líneas de transmisión de alto voltaje en donde también se realizaron los ensambles de prototipos para su revisión y las pruebas de cargas antes de la construcción en serie de una estructura de acero con especificaciones para licitación internacional para la obra de la Línea Eléctrica Milagro- Machala. En las primeras partes de este trabajo se revisan los criterios técnicos que s...

  12. Libraries in 17th century Nueva Granada: The library of Fray Cristóbal de Torres at Colegio Mayor de Nuestra Señora del Rosario

    Directory of Open Access Journals (Sweden)

    María del Rosario García

    2015-07-01

    Full Text Available This article comprises a visit to the library of Fray Cristóbal de Torres, an exemplary reader in 17th century Nueva Granada, Archbishop of Santafé de Bogotá and founder of Colegio Mayor de Nuestra Señora del Rosario. The nature of this library is analyzed in comparison to others from the time, as well as some aspects related to the circulation of knowledge in Spain and America, through the analysis of the texts donated to Colegio Mayor del Rosario by the archbishop. Likewise, this article presents some conclusions regarding the conditions of education in 17th century Nueva Granada.

  13. Pedro de la Torre y los retablos baldaquino de la Virgen del Sagrario de Toledo y de los Ojos Grandes de Lugo

    Directory of Open Access Journals (Sweden)

    Adolfo de Abel Vilela

    1995-01-01

    Full Text Available La introducción en Galicia del retablo-baldaquino, difundido por la escuela madrileña del siglo XVII, tendrá lugar a través de una traza hecha por su creador Pedro de la Torre para la Virgen del Sagrario de la catedral de Toledo. Esta obra de la catedral de Lugo, hoy desaparecida, se hará, con algunas modificaciones, por el entallador y arquitecto castellano Francisco González, destinada a albergar la imagen de la Virgen de los Ojos Grandes.

  14. Integral system for computer aided design of power line transmission towers; Sistema integral para el diseno asistido por computadora de torres de transmision

    Energy Technology Data Exchange (ETDEWEB)

    Nagore, Gabriel [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1986-12-31

    The profitable use of the advantages offered by the computer aided design, through the integral system for the structural design of power transmission towers (SIDETT) will contribute to facilitate and improve the design of the structures that link the electric power generation centers with the consumers. [Espanol] El aprovechamiento de las ventajas que ofrece el diseno asistido por computadora, a traves del sistema integral para el diseno estructural de torres de transmision (SIDETT), contribuira a agilizar y mejorar el diseno de las estructuras que enlazan los centros de generacion de energia electrica con los consumidores.

  15. Mechanism of TNFα Regulating KLF4 Expression in Breast Cancer Cells SK-BR-3%肿瘤坏死因子α调节乳腺癌SK-BR-3细胞中KLF4表达及其机制研究

    Institute of Scientific and Technical Information of China (English)

    刘彩云; 史建红; 周金飒; 赵明智; 王冰; 崔乃鹏; 陈保平

    2015-01-01

    目的 探讨肿瘤坏死因子α(TNFα)对乳腺癌SK-BR-3细胞中Krüppel样因子4(KLF4)表达的影响,明确KLF4在促进乳腺癌SK-BR-3细胞凋亡中的作用机制.方法 用不同浓度TNFα(0、1、5、10、20 ng/ml)刺激乳腺癌SK-BR-3细胞,采用Western blot法检测KLF4表达水平;用流式细胞术和DAPI染色法分析细胞凋亡情况.结果 随着刺激浓度的增高,KLF4表达水平呈剂量依赖性逐渐增多.流式细胞术和DAPI染色显示,TNFα可诱导乳腺癌SK-BR-3细胞凋亡.构建pAd-GFP和pAd-GFP-KLF4腺病毒表达载体,在乳腺癌SK-BR-3细胞中过表达GFP或GFP-KLF4,再给予TNFα刺激后,流式细胞术观察结果显示,KLF4过表达可促进乳腺癌SK-BR-3细胞凋亡.结论 TNFα可诱导乳腺癌SK-BR-3细胞中KLF4表达,KLF4参与了TNFα诱导的乳腺癌SK-BR-3细胞凋亡过程.

  16. Rapid agonist-induced loss of sup 125 I-. beta. -endorphin opioid receptor sites in NG108-15, but not SK-N-SH neuroblastoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Cone, R.I.; Lameh, J.; Sadee, W. (Univ. of California, San Francisco (United States))

    1991-01-01

    The authors have measured {mu} and {delta} opioid receptor sites on intact SK-N-SH and NG108-15 neuroblastoma cells, respectively, in culture. Use of {sup 125}I-{beta}-endorphin ({beta}E) as a tracer, together with {beta}E(6-31) to block high-affinity non-opioid binding in both cell lines, permitted the measurement of cell surface {mu} and {delta} opioid receptor sites. Labeling was at {delta} sites in NG108-15 cells and predominantly at {mu} sites in SK-N-SH cells. Pretreatment with the {mu} and {delta} agonist, DADLE, caused a rapid loss of cell surface {delta} receptor sites in NG108-15 cells, but failed to reduce significantly {mu} receptor density in SK-N-SH cells.

  17. Carcino-embryonic antigen in monitoring the growth of human colon adenocarcinoma tumour cells SK-CO-1 and HT-29 in vitro and in nude mice

    DEFF Research Database (Denmark)

    Sölétormos, G; Fogh, J M; Sehested-Hansen, B

    1997-01-01

    A set of experimental model systems were designed to investigate (a) the inter-relationship between growth of two human cancer cell lines (SK-CO-1, HT-29) and carcino-embryonic antigen (CEA) kinetics; and (b) whether neoplastic growth or CEA concentration is modulated by human growth hormone (h......GH). We found that increasing CEA concentration depended on tumour burden. SK-CO-1 cells had the lowest growth rates but the highest rates of CEA production. The rate of CEA increase exceeded the growth rate of both SK-CO-1 and HT-29. hGH modulated neither neoplastic growth nor CEA production....... In conclusion, our results suggest that experimental models may be useful for investigating the role of serological markers as monitors of increasing tumour burden. It will be of interest to investigate the performance of those model systems in examining the effect of cytotoxic agents in neoplastic growth....

  18. Krüppel-like factor 4 induces apoptosis and inhibits tumorigenic progression in SK-BR-3 breast cancer cells.

    Science.gov (United States)

    Wang, Bing; Zhao, Ming-Zhi; Cui, Nai-Peng; Lin, Dan-Dan; Zhang, An-Yi; Qin, Yan; Liu, Cai-Yun; Yan, Wei-Tao; Shi, Jian-Hong; Chen, Bao-Ping

    2015-01-01

    Krüppel-like factor 4 (KLF4) functions as either a tumor suppressor or an oncogene in different tissues by regulating the expression of various genes. The aim of this study was to reveal the functions of KLF4 in regulating breast cancer apoptosis, proliferation, and tumorigenic progression. KLF4 expression levels in breast cancer tissues and breast cancer cell lines were found to be much lower than those in nontumorous tissues and a nontransformed mammary epithelial cell line. KLF4 was upregulated in the tumor necrosis factor-α-induced SK-BR-3 breast cancer cell apoptotic process. Overexpression of KLF4 promoted SK-BR-3 breast cancer cell apoptosis and suppressed SK-BR-3 cell tumorigenicity in vivo.

  19. RuvBL2 is involved in histone deacetylase inhibitor PCI-24781-induced cell death in SK-N-DZ neuroblastoma cells.

    Directory of Open Access Journals (Sweden)

    Qinglei Zhan

    Full Text Available Neuroblastoma is the second most common solid tumor diagnosed during infancy. The survival rate among children with high-risk neuroblastoma is less than 40%, highlighting the urgent needs for new treatment strategies. PCI-24781 is a novel hydroxamic acid-based histone deacetylase (HDAC inhibitor that has high efficacy and safety for cancer treatment. However, the underlying mechanisms of PCI-24781 are not clearly elucidated in neuroblastoma cells. In the present study, we demonstrated that PCI-24781 treatment significantly inhibited tumor growth at very low doses in neuroblastoma cells SK-N-DZ, not in normal cell line HS-68. However, PCI-24781 caused the accumulation of acetylated histone H3 both in SK-N-DZ and HS-68 cell line. Treatment of SK-N-DZ with PCI-24781 also induced cell cycle arrest in G2/M phase and activated apoptosis signaling pathways via the up-regulation of DR4, p21, p53 and caspase 3. Further proteomic analysis revealed differential protein expression profiles between non-treated and PCI-24781 treated SK-N-DZ cells. Totally 42 differentially expressed proteins were identified by MALDI-TOF MS system. Western blotting confirmed the expression level of five candidate proteins including prohibitin, hHR23a, RuvBL2, TRAP1 and PDCD6IP. Selective knockdown of RuvBL2 rescued cells from PCI-24781-induced cell death, implying that RuvBL2 might play an important role in anti-tumor activity of PCI-24781 in SK-N-DZ cells. The present results provide a new insight into the potential mechanism of PCI-24781 in SK-N-DZ cell line.

  20. Slit2 and Robo1 induce opposing effects on metastasis of hepatocellular carcinoma Sk-hep-1 cells.

    Science.gov (United States)

    Yuan, Mingjing; Guo, Hui; Li, Jing; Sui, Chengzhi; Qin, Ying; Wang, Jingjing; Khan, Yasir Hayat; Ye, Liying; Xie, Fuan; Wang, Heng; Yuan, Li; Ye, Jun

    2016-07-01

    The neural guidance molecular, Slit2, and its cognate receptor, Robo1, play critical roles in the development of the nervous system, nevertheless, their functions are not limited to this system. Numerous studies have shown decreased Slit2 expression in a wide variety of cancers, highlighting its potential as a tumor suppressor. However, the Slit2/Robo1 signaling axis was reported to induce either suppressive or stimulatory effects on tumor growth and metastasis, depending on cellular context. There is a paucity of information on the effects of the Slit2/Robo1 signaling axis on the growth and metastasis of human hepatocellular carcinoma (HCC). Large-scale data mining of the Oncomine database has revealed heterogeneous expression of Slit2 in HCC. We screened the Sk-hep-1, a cell line showing a relatively high level of Slit2, and low level of Robo1 expression. After Slit2 knockdown and Robo1 overexpression in these cells, we found Slit2 and Robo1 exerted opposing effects on tumor growth and metastasis both in in vitro and in vivo models. Slit2 knockdown and Robo1 overexpression in Sk-hep-1 cells promoted tumor growth and metastasis, suggesting a negative and positive role for Slit2 and Robo1, respectively, in tumor progression. Robo1 overexpression upregulated matrix metalloproteinase (MMP)2, -9 and membrane-type1 MMP (MT1-MMP) expression, stimulated MMP2, but not MMP9 activation, and downregulated expression of TIMP1 and 2. The PI3K/Akt signaling pathway is of importance in regulating MMP2 expression in Sk-hep-1 cells, since Robo1 overexpression stimulated phosphorylation of Akt while the PI3K inhibitor LY294002, significantly inhibited the upregulation of MMP2 and also the enhanced cell invasion induced by Robo1 overexpression. We postulate that Robo1 promotes tumor invasion partly by the upregulation of MMP2 after activation of PI3K/Akt signaling pathway. Notably, Slit2 knockdown caused the upregulation of Robo1 expression both at the mRNA and protein levels

  1. Two-Particle Correlations in pp, $\\overline{p}\\overline{p}$ and $K^{0}_{s}K^{0}_{s}$ pairs from Hadronic Z decays

    CERN Document Server

    Schael, S; Brunelière, R; De Bonis, I; Décamp, D; Goy, C; Jézéquel, S; Lees, J P; Martin, F; Merle, E; Minard, M N; Pietrzyk, B; Trocmé, B; Bravo, S; Casado, M P; Chmeissani, M; Crespo, J M; Fernández, E; Fernández-Bosman, M; Garrido, L; Martínez, M; Pacheco, A; Ruiz, H; Colaleo, A; Creanza, D; De Filippis, N; De Palma, M; Iaselli, G; Maggi, G; Maggi, M; Nuzzo, S; Ranieri, A; Raso, G; Ruggieri, F; Selvaggi, G; Silvestris, L; Tempesta, P; Tricomi, A; Zito, G; Huang, X; Lin, J; Ouyang, Q; Wang, T; Xie, Y; Xu, R; Xue, S; Zhang, J; Zhang, L; Zhao, W; Abbaneo, D; Barklow, T; Buchmüller, O L; Cattaneo, M; Clerbaux, B; Drevermann, H; Forty, R W; Frank, M; Gianotti, F; Hansen, J B; Harvey, J; Hutchcroft, D E; Janot, P; Jost, B; Kado, M; Mato, P; Moutoussi, A; Ranjard, F; Rolandi, Luigi; Schlatter, W D; Sguazzoni, G; Teubert, F; Valassi, A; Videau, I; Badaud, F; Dessagne, S; Falvard, A; Fayolle, D; Gay, P; Jousset, J; Michel, B; Monteil, S; Pallin, D; Pascolo, J M; Perret, P; Hansen, J D; Hansen, J R; Hansen, P H; Kraan, A C; Nilsson, B S; Kyriakis, A; Markou, C; Simopoulou, E; Vayaki, A; Zachariadou, K; Blondel, A; Brient, J C; Machefert, F P; Rougé, A; Videau, H L; Ciulli, V; Focardi, E; Parrini, G; Antonelli, A; Antonelli, M; Bencivenni, G; Bossi, F; Capon, G; Cerutti, F; Chiarella, V; Laurelli, P; Mannocchi, G; Murtas, G P; Passalacqua, L; Kennedy, J; Lynch, J G; Negus, P; O'Shea, V; Thompson, A S; Wasserbaech, S R; Cavanaugh, R J; Dhamotharan, S; Geweniger, C; Hanke, P; Hepp, V; Kluge, E E; Putzer, A; Stenzel, H; Tittel, K; Wunsch, M; Beuselinck, R; Cameron, W; Davies, G; Dornan, P J; Girone, M; Marinelli, N; Nowell, J; Rutherford, S A; Sedgbeer, J K; Thompson, J C; White, R; Ghete, V M; Girtler, P; Kneringer, E; Kuhn, D; Rudolph, G; Bouhova-Thacker, E; Bowdery, C K; Clarke, D P; Ellis, G; Finch, A J; Foster, F; Hughes, G; Jones, R W L; Pearson, M R; Robertson, N A; Smizanska, M; van der Aa, O; Delaere, C; Leibenguth, G; Lemaître, V; Blumenschein, U; Hölldorfer, F; Jakobs, K; Kayser, F; Kleinknecht, K; Müller, A S; Renk, B; Sander, H G; Schmeling, S; Wachsmuth, H W; Zeitnitz, C; Ziegler, T; Bonissent, A; Coyle, P; Curtil, C; Ealet, A; Fouchez, D; Payre, P; Tilquin, A; Ragusa, F; David, A; Dietl, H; Ganis, G; Hüttmann, K; Lütjens, G; Männer, W; Moser, H G; Settles, R; Villegas, M; Wolf, G; Boucrot, J; Callot, O; Davier, M; Duflot, L; Grivaz, J F; Heusse, P; Jacholkowska, A; Serin, L; Veillet, J J; Azzurri, P; Bagliesi, G; Boccali, T; Foà, L; Giammanco, A; Giassi, A; Ligabue, F; Messineo, A; Palla, F; Sanguinetti, G; Sciabà, A; Spagnolo, P; Tenchini, R; Venturi, A; Verdini, P G; Awunor, O; Blair, G A; Cowan, G; García-Bellido, A; Green, M G; Medcalf, T; Misiejuk, A; Strong, J A; Teixeira-Dias, P; Clifft, R W; Edgecock, T R; Norton, P R; Tomalin, I R; Ward, J J; Bloch-Devaux, B; Boumediene, D E; Colas, P; Fabbro, B; Lançon, E; Lemaire, M C; Locci, E; Pérez, P; Rander, J; Tuchming, B; Vallage, B; Litke, A M; Taylor, G; Booth, C N; Cartwright, S; Combley, F; Hodgson, P N; Lehto, M H; Thompson, L F; Böhrer, A; Brandt, S; Grupen, C; Hess, J; Ngac, A; Prange, G; Borean, C; Giannini, G; He, H; Pütz, J; Rothberg, J E; Armstrong, S R; Berkelman, K; Cranmer, K; Ferguson, D P S; Gao, Y; González, S; Hayes, O J; Hu, H; Jin, S; Kile, J; McNamara, P A; Nielsen, J; Pan Yi Bin; Von Wimmersperg-Töller, J H; Wiedenmann, W; Wu, J; Wu Sau Lan; Wu, X; Zobernig, G; Dissertori, G

    2005-01-01

    Two-Particle correlations in pp, pbarpbar and K0sK0s pairs have been studied in hadronic Z decays recorded at LEP with the ALEPH detector. The correlations were measured as a function of the four-momentum difference Q of the pair. For pp, pbarpbar pairs a depletion of events is observed in the region Q < 3 GeV, and for K0sK0s pairs an enhancement of events is observed in the region Q < 0.5 GeV. These features are consistent with expectations from Fermi-Dirac and Bose-Einstein statistics, respectively.

  2. TIMP-1 overexpression does not affect sensitivity to HER2-targeting drugs in the HER2-gene-amplified SK-BR-3 human breast cancer cell line

    DEFF Research Database (Denmark)

    Deng, Xiaohong; Fogh, Louise; Lademann, Ulrik Axel

    2013-01-01

    affect sensitivity to the HER2-targeting drugs trastuzumab and lapatinib. SK-BR-3 human breast cancer cells were stably transfected with TIMP-1, characterized with regard to TIMP-1 protein expression, proliferation, and functionality of the secreted TIMP-1, and the sensitivity to trastuzumab...... and lapatinib was studied in five selected single-cell subclones expressing TIMP-1 protein at various levels plus the parental SK-BR-3 cell line. Both trastuzumab and lapatinib reduced cell viability, as determined by MTT assay, but the sensitivity to the drugs was not associated with the expression level...

  3. Egf binding to its receptor triggers a rapid tyrosine phosphorylation of the erbB-2 protein in the mammary tumor cell line SK-BR-3.

    OpenAIRE

    King, C. R.; Borrello, I; Bellot, F; Comoglio, P; Schlessinger, J

    1988-01-01

    The epidermal growth factor receptor (EGF-R) and the erbB-2 proto-oncogene product protein are closely related by their structural homology and their shared enzymatic activity as autophosphorylating tyrosine kinases. We show that in mammary tumor cells (SK-BR-3) EGF causes a rapid increase in tyrosine phosphorylation of the erbB-2 protein. Phosphorylation of erbB-2 does not occur in cells lacking the EGF-R (MDA-MB-453). Phosphorylation of erbB-2 in SK-BR-3 cells is blocked if EGF is prevented...

  4. Antonio Sánchez Barbudo y Guillermo de Torre. Una polémica durante la Guerra Civil española

    Directory of Open Access Journals (Sweden)

    Raúl Illescas

    2006-11-01

    Full Text Available Antonio Sánchez Barbudo and Guillermo de Torre maintained during 1937 a controversy around the art and to the social function of the intellectual and the artist. This one was provoked from an article published by Guillermo de Torre in Sur magazine, that it deserved the immediate answer of Bearded Sanchez as its secretary of the magazine Hora de España; and soon a second article of the theoretician of the vanguards. The controversy, like the one that maintained the artists Ramón Gaya and José Renau is exemplary in as much it allows to read the conditions of creation and the place of the commitment of the artist and intellectual, during the last moments of II Republic and in the middle of the Civil War. This work is part of an article in course that analyzes the discussion between intellectual outstanding in Sur magazine, in where two precise and differentiated positions specify affluent that they extend the context of the Spanish conflict.

  5. Implementación de aritmética de torres de campos finitos binarios de extensión 2 en FPGA

    Directory of Open Access Journals (Sweden)

    Fabián Velásquez Clavijo

    2013-01-01

    Full Text Available En el presente trabajo se muestran los aspectos básicos de la aritmética de campos finitos binarios GF(2m, extendidos usando el concepto de torres de campos GF(22m, en este caso con extensión 2 o cuadrática. El uso de torres de campos agiliza el cómputo de operaciones en los campos finitos, lo cual es aplicado en el cálculo de emparejamientos bilineales, parte fundamental de la criptografía basada en identidad; se presentan los conceptos básicos de aritmética en GF(2m y la construcción de las operaciones suma y multiplicación en campos binarios extendidos. De igual manera, se presentan los resultados de la implementación en un dispositivo FPGA XV5LX110T de Xilinx Inc., desarrollada usando lenguaje VHDL y la herramienta ISE Design Suite System Edition 14.4.

  6. La fauna de mamíferos del yacimiento de la Campa Torres (Gijón, Asturias, España

    Directory of Open Access Journals (Sweden)

    Corina LIESAU VON LETTOW-VORBECK

    2009-12-01

    Full Text Available RESUMEN: El análisis de los restos faunísticos del castro astur de La Campa Torres ha permitido aproximarnos a las estrategias paleoeconómicas de uno de los asentamientos asturianos fortificados más importantes de la Edad del Hierro. El vacuno supone el principal recurso ganadero de la ocupación prerromana complementada, marginalmente, con el ganado lanar, caprino y porcino. La escasa frecuencia de los restos de caballo y perro constatan un patrón habitual en la mayoría de los yacimientos protohistóricos, aunque el despiece exhaustivo de algunos canes no descarta el consumo de estos carnívoros. La actividad cinegética resulta ser testimonial como complemento a la dieta, representada tan sólo por el ciervo.ABSTRACT: The study of the faunal remains of Campa Torres has allowed to approximate us to the paleoeconomical patterns of one of the most important fortified Iron Age hillforts in Asturias. The cattle supposes the main stock during the prerroman occupation, complemented marginally with the sheep, goat and pig. The low frequency of the horse and dog bones states a habitual pattern as in most of the prehistoric sites, although the exhaustive disarticulation marks of dogs do not discard the consume of these carnivores. The hunting activities are evidenced only by some testimonial remains of red dear.

  7. Estudio del patrimonio cultural del valle de Echo en Huesca: la calzada romana Zaragoza-Bearne y la torre de Ysil.

    Directory of Open Access Journals (Sweden)

    Torreño Gómez, Ildefonso

    2002-08-01

    Full Text Available In this article, studies and analysis of pathology as well as consolidation and rehabilitation proposals of the roman road "Caesaraugusta-Beneharnum " are presented. The study focuses on the Northern part of this road, located in the Echo Valley, and the guard tower of Ysil placed there. This research has been accomplished in order to establish a tourist trail on this roman road, restauring the tower as un information stand for the trailEn este artículo se presentan los trabajos de estudio y análisis de patología y propuestas de consolidación y rehabilitación de la calzada romana "Caesaraugusta-Beneharnum", en su tramo situado al norte del Valle de Echo (Huesca, y de la torre-vigía de Ysil, enclavada en la misma. Estos trabajos fueron realizados con el objeto de habilitar una senda turística en la mencionada calzada, y rehabilitar la torre de Ysil como punto de parada e información sobre la misma.

  8. The reconstruction of air pressure in Gdańsk in the period of instrumental observations, 1739-2012

    Science.gov (United States)

    Filipiak, Janusz

    2015-04-01

    The purpose of the paper is to characterise the details of reconstruction of air pressure in Gdańsk in the whole period of regular instrumental records spanning the period 1739-2012. Gdańsk pressure series has been reconstructed by joining air pressure observations of 15 local shorter series. The entire instrumental series can be divided into three sub-periods: the Early Observers period, lasting to 1806, the First Meteorological Networks, covering the years from 1807 to 1875 and Modern Measurements begun in 1876. During the first period, observations were made from two to four times per day, sometimes even at midnight. In the 19th century and up to 1945 thrice-daily barometer readings were a standard, in the last few decades the density of data is much denser. A serious gap in the original daily data exists for the period 1849-1875, where it appears that no original source of data have survived. Selected data are presented in the form of five-day or monthly averages. Numerous errors made probably during the re-writing of original observers' data by their assistants were discovered during digitalization. Despite this the quality of observations can be regarded as good. Data have been corrected to provide a monthly-mean measure of atmospheric pressure in the unit of hPa at standard conditions, i.e. standard gravity, 0 °C and at sea level. Some inconsistencies may still arise as the procedure of the homogenization of air temperature is not completed and in case of a few oldest series air temperature was estimated. Numerous breakpoints were identified in the homogenisation of the series and they cannot be always linked to known causes. A reasonably detailed station history has been compiled by incorporating metadata contained in various written sources. These metadata have facilitated the homogenisation of the data series. Mean annual atmospheric pressure in Gdańsk in period 1739-2012 calculated on the basis of homogenized series was 1014.9 hPa. Mean seasonal

  9. Analysis of PolSK based FSO system using wavelength and time diversity over strong atmospheric turbulence with pointing errors

    Science.gov (United States)

    Prabu, K.; Cheepalli, Shashidhar; Kumar, D. Sriram

    2014-08-01

    Free space optics (FSO) or wireless optical communication systems is an evolving alternative to the current radio frequency (RF) links due to its high and secure datarates, large license free bandwidth access, ease of installation, and lower cost for shorter range distances. These systems are largely influenced by atmospheric conditions due to wireless transmission; requirement of line of sight (LOS) propagation may lead to alignment problems in turn pointing errors. In this paper, we consider atmospheric turbulence and pointing errors are the major limitations. We tried to address these difficulties by considering polarization shift keying (PolSK) modulated FSO communication system with wavelength and time diversity. We derived the closed form expressions for estimation of the average bit error rate (BER) and outage probability, which are vital system performance metrics. Analytical results are shown considering different practical cases.

  10. 绝地大反攻击败SK NiP瑞典称霸王者再现

    Institute of Scientific and Technical Information of China (English)

    J.son

    2006-01-01

    <正> NiP 自2005年由 HeatoN 及 Potti 重组以来,仍然活跃在世界 CS 舞台,但却始终缺乏一种王者之气,即便是在瑞典国内,也未曾取得过令人瞩目的成绩。而 SK 虽然也几经重组,但仍然依靠着 Fisker 和 Spawn 等老将的稳定发挥保住了自己的霸主地位。但就在上个月,Spawn 突然由 SK 出走并进入了 NiP 的阵容,而且临时取代了 HeatoN 的位置。突如其来的变故使人们一下子关注起 NiP 来,毕竟 NiP 的阵容已经更趋向稳定和合理。两个豪门俱乐部的恩恩怨怨向来为世界 CS 爱好者们所津津乐道,如今新仇旧恨交织在一起的10个人将爆发出一场怎样的战争呢? NiP 自2005年由 HeatON 及 Potti 重组以来,仍然活跃在世界 CS 舞台,但却始终缺乏一种王者之气,即便是在瑞典国内,也未曾取得过令人瞩目的成绩。而 SK 虽然也几经重组,但仍然依靠着 Fisker 和 Spawn 等老将的稳定发挥保住了自己的霸主地位。但就在上个月,Spawn 突然由 SK 出走并进入了 NiP 的阵容,而且临时取代了 HeatON 的位置。突如其来的变故使人们一下子关注起 NiP 来,毕竟 NiP 的阵容已经更趋向稳定和合理。两个豪门俱乐部的恩恩怨怨向来为世界 CS 爱好者们所津津乐道,如今新仇旧恨交织在一起的10个人将爆发出一场怎样的战争呢?

  11. Polonium (210)Po activities in human blood of patients with ischaemic heart disease from Gdańsk in Poland.

    Science.gov (United States)

    Boryło, Alicja; Skwarzec, Bogdan; Romańczyk, Grzegorz; Siebert, Janusz

    The determination of polonium (210)Po in human blood samples is presented and discussed in this paper. The human blood samples were collected from patients of Medical University of Gdańsk with ischaemic heart disease (morbus ischaemicus cordis, MIC). The polonium concentrations in analyzed human blood samples are very differentiated. (210)Po is of particular interest in public health and although is present in the environment in extremely low amounts, it is easily bioaccumulated to the human body. The study shows that the amount of (210)Po that is incorporated into the human body depends on the food habits and some difference in its levels could be observed between smokers and non-smokers.

  12. COMPARATIVE ANALYSIS OF TRANSPORT ORGANIZATION AND FUNCTIONING OF WATER TRAMS IN GDAŃSK, BYDGOSZCZ AND KRAKÓW

    Directory of Open Access Journals (Sweden)

    Emilia Miszewska-Urbańsk

    2016-03-01

    Full Text Available The subject of this work is to identify and juxtapose solutions implemented in transport provided by water trams for the following cities: Gdańsk, Bydgoszcz and Kraków. As a result of the conducted analysis of transport factors, the degree of conformity of project objectives to the actual role of the water tram has been determined. It was found that the Kraków Water Tram (also known as Cracow Water Tram is characterized by the greatest degree of conformity with project objectives. The solutions adopted in the project can be used to develop organizational and functional standards for this type of transport in Poland, since the lack of system solutions and little experience in implementation of water trams resulted in different organizational and technical approaches in their functioning.

  13. Selegiline increases heme oxygenase-1 expression and the cytotoxicity produced by dopamine treatment of neuroblastoma SK-N-SH cells

    Directory of Open Access Journals (Sweden)

    Rieder C.R.M.

    2004-01-01

    Full Text Available Increased dopamine catabolism may be associated with oxidative stress and neuronal cell death in Parkinson's disease. The present study was carried out to examine the effect of dopamine on the expression of heme oxygenase-1 and -2 (HO-1 and HO-2 in human neuroblastomas (SK-N-SH cell line and the effects of selegiline and antioxidants on this expression. Cells were kept with close control of pH and were incubated with varying concentrations of dopamine (0.1-100 µM for 24 h. HO-1 and HO-2 cDNA probes were prepared by reverse transcription-polymerase chain reaction amplification. The mRNA expression of HO-1 and HO-2 was measured by Northern blot analysis. The levels of HO-1 mRNA increased after dopamine treatment, in a dose-dependent manner, in all cell lines studied, whereas levels of the two HO-2 transcripts did not. The HO-1 and HO-2 protein expression was analyzed by Western blotting. HO-1 protein was undetectable in untreated SK-N-SH cells and increased after treatment with dopamine. In contrast, the HO-2 protein (36 kDa was detected in untreated cells and the levels did not change as a result of treatment. alpha-Tocopherol (10-100 µM and ascorbic acid (100 µM did not attenuate the effects of dopamine. Selegiline (10 µM produced significant increase (P < 0.01 in the induction of HO-1 by dopamine (more than six times the control values. The increased expression of HO-1 following dopamine treatment indicates that dopamine produces oxidative stress in this cell line.

  14. Impact of image segmentation on high-content screening data quality for SK-BR-3 cells

    Directory of Open Access Journals (Sweden)

    Li Yizheng

    2007-09-01

    Full Text Available Abstract Background High content screening (HCS is a powerful method for the exploration of cellular signalling and morphology that is rapidly being adopted in cancer research. HCS uses automated microscopy to collect images of cultured cells. The images are subjected to segmentation algorithms to identify cellular structures and quantitate their morphology, for hundreds to millions of individual cells. However, image analysis may be imperfect, especially for "HCS-unfriendly" cell lines whose morphology is not well handled by current image segmentation algorithms. We asked if segmentation errors were common for a clinically relevant cell line, if such errors had measurable effects on the data, and if HCS data could be improved by automated identification of well-segmented cells. Results Cases of poor cell body segmentation occurred frequently for the SK-BR-3 cell line. We trained classifiers to identify SK-BR-3 cells that were well segmented. On an independent test set created by human review of cell images, our optimal support-vector machine classifier identified well-segmented cells with 81% accuracy. The dose responses of morphological features were measurably different in well- and poorly-segmented populations. Elimination of the poorly-segmented cell population increased the purity of DNA content distributions, while appropriately retaining biological heterogeneity, and simultaneously increasing our ability to resolve specific morphological changes in perturbed cells. Conclusion Image segmentation has a measurable impact on HCS data. The application of a multivariate shape-based filter to identify well-segmented cells improved HCS data quality for an HCS-unfriendly cell line, and could be a valuable post-processing step for some HCS datasets.

  15. Anthocyanins Protect SK-N-SH Cells Against Acrolein-Induced Toxicity by Preserving the Cellular Redox State.

    Science.gov (United States)

    Belkacemi, Abdenour; Ramassamy, Charles

    2016-01-01

    In Alzheimer's disease (AD) and in mild cognitive impairment (MCI) patients, by-products of lipid peroxidation such as acrolein accumulated in vulnerable regions of the brain. We have previously shown that acrolein is a highly reactive and neurotoxic aldehyde and its toxicity involves the alteration of several redox-sensitive pathways. Recently, protein-conjugated acrolein in cerebrospinal fluid has been proposed as a biomarker to distinguish between MCI and AD. With growing evidence of the early involvement of oxidative stress in AD etiology, one would expect that a successful therapy should prevent brain oxidative damage. In this regard, several studies have demonstrated that polyphenol-rich extracts exert beneficial effect on cognitive impairment and oxidative stress. We have recently demonstrated the efficacy of an anthocyanin formulation (MAF14001) against amyloid-β-induced oxidative stress. The aim of this study is to investigate the neuroprotective effect of MAF14001 as a mixture of anthocyanins, a particular class of polyphenols, against acrolein-induced oxidative damage in SK-N-SH neuronal cells. Our results demonstrated that MAF14001, from 5μM, was able to efficiently protect SK-N-SH cells against acrolein-induced cell death. MAF14001 was able to lower reactive oxygen species and protein carbonyl levels induced by acrolein. Moreover, MAF1401 prevented glutathione depletion and positively modulated, in the presence of acrolein, some oxidative stress-sensitive pathways including the transcription factors NF-κB and Nrf2, the proteins γ-GCS and GSK3β, and the protein adaptator p66Shc. Along with its proven protective effect against amyloid-β toxicity, these results demonstrate that MAF14001 could target multiple mechanisms and could be a promising agent for AD prevention.

  16. MULTICELLULAR-MEDIATED RESISTANCE TO CISPLATIN AND TAXOL IN HUMAN OVARIAN CANCER SK-OV-3IP1 MULTICELLULAR AGGREGATES

    Institute of Scientific and Technical Information of China (English)

    陈建利; 丰有吉; 张琴

    2002-01-01

    Objective: To investigate the chemosensitivity of ovarian cancer SK-OV-3ip1 multicellular aggregates (MCA) to cisplatin and taxol and to explore the possible mechanisms. Methods: Liquid overlay system was employed to obtain MCA. We detected the resistance using trypan blue exclusion testing, clonogenic assay, cell cycle profiles and apoptosis with flow cytometry (FCM). Results: After cisplatin exposure, MCA cells showed nearly equal cell viability with monolayer cells (P=0.05). After 40(M cisplatin exposure for 12 h, no clone ((50 cells) was formed, but more viable cells attached to the bottom of 24-well plate in MCA group than monolayer. Furthermore, apoptosis rate and cell cycle profiles with FCM had no significant change between MCA and monolayer cells. After taxol exposure, however, trypan blue exclusion testing demonstrated higher cell viability in MCA cells (P=0.003) and higher clone formation rate in 100-cell group than monolayer cells (0.01SK-OV-3ip1 cell line appear to be more resistant to taxol but not to cisplatin. Cell cycle redistribution and multicellular-mediated inhibition of apoptosis can partially account for the resistance.

  17. A High Molecular-Mass Anoxybacillus sp. SK3-4 Amylopullulanase: Characterization and Its Relationship in Carbohydrate Utilization

    Directory of Open Access Journals (Sweden)

    Kian Mau Goh

    2013-05-01

    Full Text Available An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK was purified to homogeneity and characterized. Though amylopullulanases larger than 200 kDa are rare, the molecular mass of purified ApuASK appears to be approximately 225 kDa, on both SDS-PAGE analyses and native-PAGE analyses. ApuASK was stable between pH 6.0 and pH 8.0 and exhibited optimal activity at pH 7.5. The optimal temperature for ApuASK enzyme activity was 60 °C, and it retained 54% of its total activity for 240 min at 65 °C. ApuASK reacts with pullulan, starch, glycogen, and dextrin, yielding glucose, maltose, and maltotriose. Interestingly, most of the previously described amylopullulanases are unable to produce glucose and maltose from these substrates. Thus, ApuASK is a novel, high molecular-mass amylopullulanase able to produce glucose, maltose, and maltotriose from pullulan and starch. Based on whole genome sequencing data, ApuASK appeared to be the largest protein present in Anoxybacillus sp. SK3-4. The α-amylase catalytic domain present in all of the amylase superfamily members is present in ApuASK, located between the cyclodextrin (CD-pullulan-degrading N-terminus and the α-amylase catalytic C-terminus (amyC domains. In addition, the existence of a S-layer homology (SLH domain indicates that ApuASK might function as a cell-anchoring enzyme and be important for carbohydrate utilization in a streaming hot spring.

  18. Carcino-embryonic antigen in monitoring the growth of human colon adenocarcinoma tumour cells SK-CO-1 and HT-29 in vitro and in nude mice

    DEFF Research Database (Denmark)

    Sölétormos, G; Fogh, J M; Sehested-Hansen, B;

    1997-01-01

    A set of experimental model systems were designed to investigate (a) the inter-relationship between growth of two human cancer cell lines (SK-CO-1, HT-29) and carcino-embryonic antigen (CEA) kinetics; and (b) whether neoplastic growth or CEA concentration is modulated by human growth hormone (h...

  19. $K^{0}_{s}-K^{0}_{s}$ correlations in pp collisions at $\\sqrt{s}=7 TeV$ from the LHC ALICE experiment

    CERN Document Server

    Abelev, Betty; Adamova, Dagmar; Adare, Andrew Marshall; Aggarwal, Madan; Aglieri Rinella, Gianluca; Agocs, Andras Gabor; Agostinelli, Andrea; Aguilar Salazar, Saul; Ahammed, Zubayer; Ahmad, Arshad; Ahmad, Nazeer; Ahn, Sang Un; Akindinov, Alexander; Aleksandrov, Dmitry; Alessandro, Bruno; Alfaro Molina, Jose Ruben; Alici, Andrea; Alkin, Anton; Almaraz Avina, Erick Jonathan; Alme, Johan; Alt, Torsten; Altini, Valerio; Altinpinar, Sedat; Altsybeev, Igor; Andrei, Cristian; Andronic, Anton; Anguelov, Venelin; Anielski, Jonas; Anticic, Tome; Antinori, Federico; Antonioli, Pietro; Aphecetche, Laurent Bernard; Appelshauser, Harald; Arbor, Nicolas; Arcelli, Silvia; Armesto, Nestor; Arnaldi, Roberta; Aronsson, Tomas Robert; Arsene, Ionut Cristian; Arslandok, Mesut; Augustinus, Andre; Averbeck, Ralf Peter; Awes, Terry; Aysto, Juha Heikki; Azmi, Mohd Danish; Bach, Matthias Jakob; Badala, Angela; Baek, Yong Wook; Bailhache, Raphaelle Marie; Bala, Renu; Baldini Ferroli, Rinaldo; Baldisseri, Alberto; Baldit, Alain; Baltasar Dos Santos Pedrosa, Fernando; Ban, Jaroslav; Baral, Rama Chandra; Barbera, Roberto; Barile, Francesco; Barnafoldi, Gergely Gabor; Barnby, Lee Stuart; Barret, Valerie; Bartke, Jerzy Gustaw; Basile, Maurizio; Bastid, Nicole; Basu, Sumit; Bathen, Bastian; Batigne, Guillaume; Batyunya, Boris; Baumann, Christoph Heinrich; Bearden, Ian Gardner; Beck, Hans; Behera, Nirbhay Kumar; Belikov, Iouri; Bellini, Francesca; Bellwied, Rene; Belmont-Moreno, Ernesto; Bencedi, Gyula; Beole, Stefania; Berceanu, Ionela; Bercuci, Alexandru; Berdnikov, Yaroslav; Berenyi, Daniel; Bergognon, Anais Annick Erica; Berzano, Dario; Betev, Latchezar; Bhasin, Anju; Bhati, Ashok Kumar; Bhom, Jihyun; Bianchi, Livio; Bianchi, Nicola; Bianchin, Chiara; Bielcik, Jaroslav; Bielcikova, Jana; Bilandzic, Ante; Bjelogrlic, Sandro; Blanco, F; Blanco, Francesco; Blau, Dmitry; Blume, Christoph; Bock, Nicolas; Boettger, Stefan; Bogdanov, Alexey; Boggild, Hans; Bogolyubsky, Mikhail; Boldizsar, Laszlo; Bombara, Marek; Book, Julian; Borel, Herve; Borissov, Alexander; Bose, Suvendu Nath; Bossu, Francesco; Botje, Michiel; Boyer, Bruno Alexandre; Braidot, Ermes; Braun-Munzinger, Peter; Bregant, Marco; Breitner, Timo Gunther; Browning, Tyler Allen; Broz, Michal; Brun, Rene; Bruna, Elena; Bruno, Giuseppe Eugenio; Budnikov, Dmitry; Buesching, Henner; Bufalino, Stefania; Bugaiev, Kyrylo; Busch, Oliver; Buthelezi, Edith Zinhle; Caffarri, Davide; Cai, Xu; Caines, Helen Louise; Calvo Villar, Ernesto; Camerini, Paolo; Canoa Roman, Veronica; Cara Romeo, Giovanni; Carena, Francesco; Carena, Wisla; Carminati, Federico; Casanova Diaz, Amaya Ofelia; Castillo Castellanos, Javier Ernesto; Casula, Ester Anna Rita; Catanescu, Vasile; Cavicchioli, Costanza; Ceballos Sanchez, Cesar; Cepila, Jan; Cerello, Piergiorgio; Chang, Beomsu; Chapeland, Sylvain; Charvet, Jean-Luc Fernand; Chattopadhyay, Sukalyan; Chattopadhyay, Subhasis; Chawla, Isha; Cherney, Michael Gerard; Cheshkov, Cvetan; Cheynis, Brigitte; Chiavassa, Emilio; Chibante Barroso, Vasco Miguel; Chinellato, David; Chochula, Peter; Chojnacki, Marek; Choudhury, Subikash; Christakoglou, Panagiotis; Christensen, Christian Holm; Christiansen, Peter; Chujo, Tatsuya; Chung, Suh-Urk; Cicalo, Corrado; Cifarelli, Luisa; Cindolo, Federico; Cleymans, Jean Willy Andre; Coccetti, Fabrizio; Colamaria, Fabio; Colella, Domenico; Conesa Balbastre, Gustavo; Conesa del Valle, Zaida; Constantin, Paul; Contin, Giacomo; Contreras, Jesus Guillermo; Cormier, Thomas Michael; Corrales Morales, Yasser; Cortes Maldonado, Ismael; Cortese, Pietro; Cosentino, Mauro Rogerio; Costa, Filippo; Cotallo, Manuel Enrique; Crochet, Philippe; Cruz Alaniz, Emilia; Cuautle, Eleazar; Cunqueiro, Leticia; D'Erasmo, Ginevra; Dainese, Andrea; Dalsgaard, Hans Hjersing; Danu, Andrea; Das, Debasish; Das, Indranil; Das, Kushal; Dash, Ajay Kumar; Dash, Sadhana; De, Sudipan; de Barros, Gabriel; De Caro, Annalisa; de Cataldo, Giacinto; de Cuveland, Jan; De Falco, Alessandro; De Gruttola, Daniele; De Marco, Nora; De Pasquale, Salvatore; de Rooij, Raoul Stefan; Delagrange, Hugues; Deloff, Andrzej; Demanov, Vyacheslav; Denes, Ervin; Deppman, Airton; Di Bari, Domenico; Di Giglio, Carmelo; Di Liberto, Sergio; Di Mauro, Antonio; Di Nezza, Pasquale; Diaz Corchero, Miguel Angel; Dietel, Thomas; Divia, Roberto; Djuvsland, Oeystein; Dobrin, Alexandru Florin; Dobrowolski, Tadeusz Antoni; Dominguez, Isabel; Donigus, Benjamin; Dordic, Olja; Driga, Olga; Dubey, Anand Kumar; Ducroux, Laurent; Dupieux, Pascal; Dutta Majumdar, AK

    2013-07-16

    Identical neutral kaon pair correlations are measured in $\\sqrt{s}$=7 TeV pp collisions in the ALICE experiment. One-dimensional K$^{0}_{s}$-K$^{0}_{s}$ correlation functions in terms of the invariant momentum difference of kaon pairs are formed in two multiplicity and two transverse momentum ranges. The femtoscopic parameters for the radius and correlation strength of the kaon source are extracted. The fit includes quantum statistics and final-state interactions of the $a_0/f_0$ resonance. K$^{0}_{s}$-K$^{0}_{s}$ correlations show an increase in radius for increasing multiplicity and a slight decrease in radius for increasing transverse mass, $m_T$, as seen in pion-pion correlations in the pp system and in heavy-ion collisions. Transverse mass scaling is observed between the K$^{0}_{s}$-K$^{0}_{s}$ and pion-pion radii. Also, the first observation is made of the decay of the $f_{2}'$(1525) meson into the K$^{0}_{s}$-K$^{0}_{s}$ channel in pp collisions.

  20. Intracellular synthesis of glutamic acid in Bacillus methylotrophicus SK19.001, a glutamate-independent poly(γ-glutamic acid)-producing strain.

    Science.gov (United States)

    Peng, Yingyun; Zhang, Tao; Mu, Wanmeng; Miao, Ming; Jiang, Bo

    2016-01-15

    Bacillus methylotrophicus SK19.001 is a glutamate-independent strain that produces poly(γ-glutamic acid) (γ-PGA), a polymer of D- and L-glutamic acids that possesses applications in food, the environment, agriculture, etc. This study was undertaken to explore the synthetic pathway of intracellular L- and D-glutamic acid in SK19.001 by investigating the effects of tricarboxylic acid cycle intermediates and different amino acids as metabolic precursors on the production of γ-PGA and analyzing the activities of the enzymes involved in the synthesis of L- and D-glutamate. Tricarboxylic acid cycle intermediates and amino acids could participate in the synthesis of γ-PGA via independent pathways in SK19.001. L-Aspartate aminotransferase, L-glutaminase and L-glutamate synthase were the enzymatic sources of L-glutamate. Glutamate racemase was responsible for the formation of D-glutamate for the synthesis of γ-PGA, and the synthetase had stereoselectivity for glutamate substrate. The enzymatic sources of L-glutamate were investigated for the first time in the glutamate-independent γ-PGA-producing strain, and multiple enzymatic sources of L-glutamate were verified in SK19.001, which will benefit efforts to improve production of γ-PGA with metabolic engineering strategies. © 2015 Society of Chemical Industry.

  1. SK channels modulate the excitability and firing precision of projection neurons in the robust nucleus of the arcopallium in adult male zebra finches

    Institute of Scientific and Technical Information of China (English)

    Guo-Qiang Hou; Xuan Pan; Cong-Shu Liao; Song-Hua Wang; Dong-Feng Li

    2012-01-01

    [Objective] Motor control is encoded by neuronal activity.Small conductance Ca2+-activated Kˉ channels (SK channels) maintain the regularity and precision of firing by contributing to the afterhyperpolarization (AHP) of the action potential in mammals.However,it is not clear how SK channels regulate the output of the vocal motor system in songbirds.The premotor robust nucleus of the arcopallium (RA) in the zebra finch is responsible for the output of song information.The temporal pattern of spike bursts in RA projection neurons is associated with the timing of the acoustic features of birdsong.[Methods] The firing properties of RA projection neurons were analyzed using patch clamp wholecell and cell-attached recording techniques.[Results] SK channel blockade by apamin decreased the AHP amplitude and increased the evoked firing rate in RA projection neurons.It also caused reductions in the regularity and precision of firing.RA projection neurons displayed regular spontaneous action potentials,while apamin caused irregular spontaneous firing but had no effect on the firing rate.In the absence of synaptic inputs,RA projection neurons still had spontaneous firing,and apamin had an evident effect on the firing rate,but caused no significant change in the firing regularity,compared with apamin application in the presence of synaptic inputs.[Conclusion]SK channels contribute to the maintenance of firing regularity in RA projection neurons whichrequires synaptic activity,and consequently ensures the precision of song encoding.

  2. METCAM/MUC18 promoted tumorigenesis of human breast cancer SK-BR-3 cells in a dosage-specific manner

    Directory of Open Access Journals (Sweden)

    Chang-Yu Huang

    2016-04-01

    Conclusion: Transient suppression observed previously in the clone was caused by injection of a high cell number (2 × 106–5 × 106. METCAM/MUC18 positively promotes tumorigenesis of SK-BR-3 cells by increasing the survival and proliferation pathway.

  3. Effect of the SK/IK channel modulator 4,5-dichloro-1,3-diethyl-1,3-dihydro-benzoimidazol-2-one (NS4591) on contractile force in rat, pig and human detrusor smooth muscle

    DEFF Research Database (Denmark)

    Nielsen, Jens Steen; Rode, Frederik; Rahbek, Mette

    2011-01-01

    • To investigate the importance of small (SK)- and intermediate (IK)-conductance Ca2(+) -activated K(+) channels on bladder function, by studying the effects of 4,5-dichloro-1,3-diethyl-1,3-dihydro-benzoimidazol-2-one (NS4591), a new modulator of SK/IK channels, on contractions induced...

  4. Therapeutic action of the mitochondria-targeted antioxidant SkQ1 on retinopathy in OXYS rats linked with improvement of VEGF and PEDF gene expression.

    Directory of Open Access Journals (Sweden)

    Anton M Markovets

    Full Text Available UNLABELLED: The incidence of age-related macular degeneration (AMD, the main cause of blindness in older patients in the developed countries, is increasing with the ageing population. At present there is no effective treatment for the prevailing geographic atrophy, dry AMD, whereas antiangiogenic therapies successful used in managing the wet form of AMD. Recently we showed that mitochondria-targeted antioxidant plastoquinonyl-decyl-triphenylphosphonium (SkQ1 is able to prevent the development and moreover caused regression of pre-existing signs of the retinopathy in OXYS rats, an animal model of AMD. Here we examine the effects of SkQ1 on expression of key regulators of angiogenesis vascular endothelial growth factor A (VEGF and its antagonist pigment epithelium-derived factor (PEDF genes in the retina of OXYS rats as evidenced by real-time PCR and an ELISA test for VEGF using Wistar rats as control. Ophthalmoscopic examinations confirmed that SkQ1 supplementation (from 1.5 to 3 months of age, 250 nmol/kg prevented development while eye drops SkQ1 (250 nM, from 9 to 12 months caused some reduction of retinopathy signs in OXYS rats and did not reveal any negative effects on the control Wistar rat's retina. Prevention of premature retinopathy by SkQ1 was connected with an increase of VEGF mRNA and protein in OXYS rat's retina up to the levels corresponding to the Wistar rats, and did not involve changes in PEDF expression. In contrast the treatment with SkQ1 drops caused a decrease of VEGF mRNA and protein levels and an increase in the PEDF mRNA level in the middle-aged OXYS rats, but in Wistar rats the changes of gene expression were the opposite. CONCLUSIONS: The beneficial effects of SkQ1 on retinopathy connected with normalization of expression of VEGF and PEDF in the retina of OXYS rats and depended on age of the animals and the stage of retinopathy.

  5. A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery.

    Science.gov (United States)

    Tamaki, Tetsuro; Hirata, Maki; Nakajima, Nobuyuki; Saito, Kosuke; Hashimoto, Hiroyuki; Soeda, Shuichi; Uchiyama, Yoshiyasu; Watanabe, Masahiko

    2016-01-01

    Losses in vital functions of the somatic motor and sensory nervous system are induced by severe long-gap peripheral nerve transection injury. In such cases, autologous nerve grafts are the gold standard treatment, despite the unavoidable sacrifice of other healthy functions, whereas the prognosis is not always favorable. Here, we use human skeletal muscle-derived stem cells (Sk-SCs) to reconstitute the function after long nerve-gap injury. Muscles samples were obtained from the amputated legs from 9 patients following unforeseen accidents. The Sk-SCs were isolated using conditioned collagenase solution, and sorted as CD34+/45- (Sk-34) and CD34-/45-/29+ (Sk-DN/29+) cells. Cells were separately cultured/expanded under optimal conditions for 2 weeks, then injected into the athymic nude mice sciatic nerve long-gap model (7-mm) bridging an acellular conduit. After 8-12 weeks, active cell engraftment was observed only in the Sk-34 cell transplanted group, showing preferential differentiation into Schwann cells and perineurial/endoneurial cells, as well as formation of the myelin sheath and perineurium/endoneurium surrounding regenerated axons, resulted in 87% of numerical recovery. Differentiation into vascular cell lineage (pericyte and endothelial cells) were also observed. A significant tetanic tension recovery (over 90%) of downstream muscles following electrical stimulation of the sciatic nerve (at upper portion of the gap) was also achieved. In contrast, Sk-DN/29+ cells were completely eliminated during the first 4 weeks, but relatively higher numerical (83% vs. 41% in axon) and functional (80% vs. 60% in tetanus) recovery than control were observed. Noteworthy, significant increase in the formation of vascular networks in the conduit during the early stage (first 2 weeks) of recovery was observed in both groups with the expression of key factors (mRNA and protein levels), suggesting the paracrine effects to angiogenesis. These results suggested that the human Sk

  6. The rock-cut tomb of Bolores (Torres Vedras: an interdisciplinary approach to understanding the social landscape of the Late Neolithic/Copper Age of the Iberian Peninsula

    Directory of Open Access Journals (Sweden)

    Lillios, Katina T.

    2014-12-01

    Full Text Available To better understand the sociopolitical landscape of the Portuguese Estremadura during the Late Neolithic/Copper Age, interdisciplinary excavations were conducted at Bolores (Torres Vedras, in the Sizandro River Valley. Following a test season in 1986, a University of Iowa team conducted four campaigns between 2007 and 2012. Bolores is a rock-cut tomb used primarily between 2800-2600 cal BC for the burial of adults, adolescents, and children (MNI=36. The architectural, material cultural, and bioarchaeological evidence suggests that Bolores housed the remains of a distinctive group of local individuals who marked their difference from other burial populations in the Sizandro and Estremadura through material culture and tomb architecture. Social differences were denoted spatially and through offerings of material goods. No social stratification is evident, however, that would suggest a state-level society: there are no wealthy child burials and no significant health or dietary disparities within this population or between it and others in the region.Para comprender mejor el paisaje sociopolítico de la Extremadura portuguesa durante el Neolítico Final/Edad del Cobre se han llevado a cabo excavaciones interdisciplinares en Bolores (Torres Vedras, en el valle del río Sizandro. Tras una campaña de prueba en 1986, un equipo de la Universidad de Iowa realizó cuatro campañas entre 2007 y 2012. Bolores es una tumba hipogea que fue utilizada principalmente entre 2800 y 2600 cal BCE como enterramiento colectivo para adultos, adolescentes y niños (NMI=36. Los datos arquitectónicos, bioarqueológicos y de cultura material analizados hasta la fecha sugieren que Bolores albergó los restos de un grupo social distintivo compuesto de individuos locales que marcaban sus diferencias con otras poblaciones funerarias del Sizandro y Extremadura portuguesa mediante la cultura material y la arquitectura funeraria. Las diferencias sociales en la población de

  7. Correção cirúrgica da doença de Hirschprung pela técnica de de la Torre-Mondragon

    Directory of Open Access Journals (Sweden)

    Gabriela Teixeira Araujo

    2015-10-01

    Full Text Available A Aganglionose Intestinal Congênita (AIC, ou Doença de Hirschprung, é uma má formação caracterizada pela ausência de células ganglionares nos plexos mioentérico e submucoso da porção distal do trato gastrointestinal. Assim, o segmento aganglionar é aperistáltico e espástico, constituindo um obstáculo ao transito intestinal e gerando a dilatação do segmento saudável. A doença costuma manifestar-se no período neonatal com obstrução intestinal, distensão abdominal e vômitos. O tratamento é cirúrgico, pela técnica de De La Torre-Mondragon, realizada em um tempo cirúrgico e sem colostomia prévia. Relato do caso: P.R.D., masculino, 33 dias de vida, foi encaminhado ao serviço com quadro de distensão abdominal e parada da eliminação de fezes há 22 horas. Foi internado primeiramente aos cinco dias de vida com quadro de obstrução intestinal, aliviada após limpeza mecânica. Encontrava-se com abdômen distendido, flácido, sem sinais de desconforto à palpação e ruídos hidroaéreos presentes. O enema opaco evidenciou progressão do contraste com dilatação do retossigmoide. A manometria retal demonstrou reflexo inibitório anorretal não desencadeado, com inúmeras evacuações do lactente durante o exame. Foi indicada correção cirúrgica feita pela técnica de De La Torre-Mondragon, sem intercorrências. Anatomopatológico da peça cirúrgica confirmou ausência de células ganglionares no segmento. O diagnóstico preciso e o manejo cirúrgico precoce foi fundamental para evitar a principal complicação desta doença, a enterocolite necrosante. A técnica de De La Torre-Mondragon consiste no rebaixamento transanal endorretal do cólon e permitiu a recuperação do transito intestinal precocemente, sem a necessidade de colostomia. O reestabelecimento precoce do transito intestinal melhorou a qualidade de vida do paciente e diminuiu custos e o tempo de hospitalização.

  8. Génesis y autoría de La torre de los siete jorobados de Emilio Carrère

    Directory of Open Access Journals (Sweden)

    Labrador Ben, Julia M.

    2002-12-01

    Full Text Available In this paper the genesis of Emilio Carrère's novel, La torre de los siete jorobados is analyzed: its bakcground as a short novel, its authorship (with the participation of a second author, Jesús de Aragón, the inclusion in this participation of some texts from other works by Carrère, the dating of the novel's precedents and follows in the form of short novels, and the consecutive editions of both the novel and of the short ones. The origin of some additional texts and their later exploitation in a new novel, La calavera de Atahualpa, are also established. A detailed study of the second novel completes the paper with an overview of the literary work of Jesús de Aragón. A brief analysis on the filmic adaptation of the novel is finally provided.Se analiza en el presente artículo la génesis de la novela de Emilio Carrère La torre de los siete jorobados: sus antecedentes como novela corta, su autoría, en la que se valora la participación de un segundo autor (Jesús de Aragón, la inclusión en dicha participación de textos de otras obras de Carrère, la datación de los antecedentes y consecuentes de dicha novela en forma de relatos cortos y de las sucesivas ediciones tanto de La torre de los siete jorobados como de dichas novelas cortas. Se establece también el origen de algunos textos adicionales y su posterior utilización en una segunda novela: La calavera de Atahualpa. El artículo se completa con un estudio detallado de la novela, un repaso somero de la obra literaria de Jesús de Aragón, y un breve análisis de la adaptación cinematográfica.

  9. Effects of magnetic c-erbB2 antisense probe on morphology and expression of SK-Br-3 cancer cell lines in vitro%磁性c-erbB2反义探针对SK-Br-3肿瘤细胞株形态及表达的影响

    Institute of Scientific and Technical Information of China (English)

    贺海容; 谭书德; 文明; 温志鹏; 刘海燕; 李少林

    2012-01-01

    目的:探讨磁性c-erbB2反义寡脱氧核苷酸探针对SK-Br-3肿瘤细胞株形态及表达的影响,阐明该探针是否同时具有诊断与治疗功能.方法:磁性c-erbB2反义探针转染高表达c-erbB2的SK Br 3肿瘤细胞作为实验组,磁性c-erbB2反义探针转染Fuda肿瘤细胞株、SPIO及ASODN转染SK-Br-3肿瘤细胞、未转染的SK-Br-3肿瘤细胞设置为4个对照组,普鲁士蓝染色光镜观察细胞内铁的分布,透射电镜观察其超微结构,并检测细胞cerbB2蛋白表达变化及铁含量,行体外MR成像.结果:光镜显示SK-Br-3肿瘤细胞胞浆内散在分布铁颗粒,透射电镜观察到SK-Br-3肿瘤细胞胞浆内铁颗粒、团状吞饮体,细胞出现胞膜空泡化、细胞固缩、核固缩及凋亡小体,而对照组细胞无上述改变;与对照组比较,实验组SK-Br3细胞蛋白的表达抑制率明显升高,细胞铁含量增加(P<0.01),MR扫描显示信号减低(P<0.05).结论:磁性c-erbB2反义探针能够顺利进入SK-Br-3肿瘤细胞,不仅能导致SK-Br-3细胞的凋亡,还能够被体外MR成像所检测,可能成为一种具有治疗与诊断意义的双功能探针.

  10. The activity of the nonsulfated sulfakinin Zopat-SK-1 in the neck-ligated larvae of the beetle Zophobas atratus.

    Science.gov (United States)

    Slocinska, M; Czubak, T; Marciniak, P; Jarmuszkiewicz, W; Rosinski, G

    2015-07-01

    Insect sulfakinins (SKs) are multifunctional neuropeptides structurally and functionally homologous to the mammalian gastrin/cholecystokinin (CCK). It has been proposed that SKs play a role in modulating energy management in insects by interacting with adipokinetic hormone (AKH), the principle hormone controlling insect intermediary metabolism. To exclude head factors (including AKH) that influence the activity of the nonsulfated sulfakinin Zopat-SK-1 in the larvae of the beetle Zophobas atratus, ligature and in vitro bioassays were used. Our study showed that in the neck-ligated larvae, Zopat-SK-1 evoked a much more pronounced glycogenolytic effect in fat body tissue and a significantly higher hypertrahelosemic effect in hemolymph than in larvae without ligation. We found that the concentration of the sugar trehalose increased under hormonal treatment but no changes in glucose levels were observed. Under in vitro conditions, the maximal glycogenolytic effect of Zopat-SK-1 in fat body was observed at 10 pmol of hormone. Ligature and in vitro bioassays indicated that Zopat-SK-1 activity in the Z. atratus larvae is modulated by head signals and/or factors from the gastrointestinal tract. Our data indicate the existence of a brain-gastrointestinal axis that has a role in controlling of energy (carbohydrate) metabolism in the insect body. Moreover, these results, together with immunological evidence of a cholecystokinin-like (sulfakinin) receptor in the Z. atratus fat body, help us to better understand the SK signaling pathways and its physiological role in insect biology. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. Thrombin/Matrix Metalloproteinase-9-Dependent SK-N-SH Cell Migration is Mediated Through a PLC/PKC/MAPKs/NF-κB Cascade.

    Science.gov (United States)

    Yang, Chien-Chung; Lin, Chih-Chung; Chien, Peter Tzu-Yu; Hsiao, Li-Der; Yang, Chuen-Mao

    2016-11-01

    Thrombin has been known to activate inflammatory genes including matrix metalloproteinases (MMPs). The elevated expression of MMP-9 has been observed in patients with neuroinflammatory diseases and may contribute to the pathology of brain diseases. However, the mechanisms underlying thrombin-induced MMP-9 expression in SK-N-SH cells remain unknown. The effects of thrombin on MMP-9 expression were examined in SK-N-SH cells by gelatin zymography, Western blot, real-time PCR, promoter activity assay, and cell migration assay. The detailed mechanisms were analyzed by using pharmacological inhibitors and small intefering RNA (siRNA) transfection. Here, we demonstrated that thrombin induced the expression of proform MMP-9 and migration of SK-N-SH cells, which were attenuated by pretreatment with the inhibitor of thrombin (PPACK), Gq (GPA2A), PC-PLC (D609), PI-PLC (ET-18-OCH3), nonselective protien kinase C (PKC, GF109203X), PKCα/βII (Gö6983), PKCδ (Rottlerin), p38 mitogen-activated protein kinases (MAPK) (SB202190), JNK1/2 (SP600125), or NF-κB (Bay11-7082 or Helenalin) and transfection with siRNA of Gq, PKCα, PKCβ, PKCδ, p38, JNK1/2, IKKα, IKKβ, or p65. Moreover, thrombin-stimulated PKCα/βII, PKCδ, p38 MAPK, JNK1/2, or p65 phosphorylation was abrogated by their respective inhibitor of PPACK, GPA2A, D609, ET-18-OCH3, Gö6983, Rottlerin, SB202190, SP600125, Bay11-7082, or Helenalin. Pretreatment with these inhibitors or transfection with MMP-9 siRNA also blocked thrombin-induced SK-N-SH cell migration. Our results show that thrombin stimulates a Gq/PLC/PKCs/p38 MAPK and JNK1/2 cascade, which in turn triggers NF-κB activation and ultimately induces MMP-9 expression and cell migration in SK-N-SH cells.

  12. KLF5在 TNFα诱导的 SK-BR-3乳腺癌细胞凋亡中的作用%Roles of KLF5 in inhibition TNFα-induced SK-BR-3 breast cancer cell apoptosis

    Institute of Scientific and Technical Information of China (English)

    史建红; 刘彩云; 张安易; 崔乃鹏; 王冰; 陈保平; 马振峰

    2014-01-01

    目的探讨肿瘤坏死因子α( TNFα)对 SK-BR-3乳腺癌细胞中 Krüppel 样因子5(KLF5)表达的影响,明确KLF5在TNFα诱导SK-BR-3乳腺癌细胞凋亡中的作用。方法用不同浓度TNFα(0、1、5、10、20μg/L)刺激SK-BR-3乳腺癌细胞不同时间(0、6、12、24、36 h),采用Western印迹方法检测KLF5表达水平;用实时定量PCR( qRT-PCR)检测TNFα刺激对SK-BR-3乳腺癌细胞凋亡基因表达的影响, qRT-PCR和流式细胞术观察过表达KLF5对SK-BR-3乳腺癌凋亡的影响。结果TNFα刺激SK-BR-3乳腺癌细胞后,随着刺激浓度的增高,KLF5表达水平呈剂量和时间依赖性逐渐降低。 qRT-PCR结果显示,TNFα可上调SK-BR-3乳腺癌细胞凋亡基因cascpase3、caspase9和bax表达,并下调bcl-2表达水平。构建pAd-GFP和pAd-GFP-KLF5腺病毒表达载体,在SK-BR-3乳腺癌细胞中过表达GFP或GFP-KLF5,再给予TNFα刺激,qRT-PCR和流式细胞术观察结果显示,过表达外源性KLF5可抑制TNFα诱导的SK-BR-3乳腺癌细胞凋亡。结论 TNFα可抑制SK-BR-3乳腺癌细胞中KLF5表达,KLF5参与了TNFα诱导的SK-BR-3乳腺癌细胞凋亡过程。%Objective To explore the expression levels and roles of Kr üpple-like factor 5 ( KLF5 ) in tumor necrosis factor α( TNFα)-induced SK-BR-3 breast cancer cells.Methods SK-BR-3 breast cancer cells were stimulated by TNFαat different concentrations (0, 1, 5, 10, 20 μg/L) for specified durations (0, 6, 12, 24, 36 h).Western blot was performed to detect KLF 5 protein levels.Then Western blot and quantitative real-time PCR ( qRT-PCR) were used to detect the expression levels of apoptosis genes.Flow cytometry and qRT-PCR were used to observe the effects of exogenous KLF 5 on TNFα-induced apoptosis of SK-BR-3 breast cancer cell.Results KLF5 expression levels significantly decreased in TNFα-stimulated SK-BR-3 breast cancer cells in a concentration-and time-dependent manner.Quantitative RT

  13. "Diagnóstico de eficiência energética de uma torre de resfriamento de água da Arcelormittal Inox Brasil"

    OpenAIRE

    2010-01-01

    O presente trabalho apresenta a aplicação de um modelo de formulação integral para determinação do parâmetro de desempenho de cada uma das três células da torre de resfriamento de água do convertedor MRP-L da área de Aciaria da ArcelorMittal Inox Brasil. Os testes foram conduzidos durante o início e o fim das corridas processadas do aço elétrico GNO, que tem boa representatividade em relação aos demais aços, tanto pela freqüência anual, quanto pelo maior calor gerado durante o seu processamen...

  14. El conocimiento geográfico de la Patagonia interior y la construcción de la imagen de Torres del Paine como patrimonio natural a conservar

    Directory of Open Access Journals (Sweden)

    Ferrer Jiménez, Daniel

    2009-06-01

    Full Text Available Torres del Paine National Park (12th Region, Chile, the Paine Massif and its surroundings are a major milestone concerning three issues: the perception of nature in the Patagonian-Andean environment, the first steps of conservationism in South America and the first scientific studies in this part of the planet. In addition, ever since scientists and travelers were to “discover” this region, Paine has been a benchmark of both the first proposals for nature tourism linked with conservationism, as well as a major challenge for alpine expeditions.

    Due to these circumstances, the doctoral dissertation of the author devotes one entire chapter to analysing the main contributions of the Paine phenomenon to the geographical knowledge of inland Patagonia and to the perception of Torres del Paine as a natural heritage worth preserving. This is precisely the subject of this paper, which gives an insight into the geographical reconnaissance explorations that took place between 1877 and 1896 in the Patagonian inland, under the initiatives of Chilean and Argentinean governments; the first known touristic travel in the Chilean region of Magallanes; the role of the first “visionaries” of Patagonian-Andean tourism in Chile, gathered around Werner Gromsch and the Touring Club; or the geographical, scientific and informative work carried out by Alberto Maria De Agostini. Finally, the article discusses the first alpine expeditions which targeted the summits of the Paine Massif.

    El Parque Nacional de las Torres del Paine (XII Región, Chile, el Macizo Paine y su entorno, constituye un hito en la imagen de la naturaleza del mundo andino patagónico, en los inicios del primer conservacionismo de América del Sur y en los estudios científicos sobre esa parte del Planeta. En relación con ello, además, el Paine ha sido, desde su “descubrimiento” científico y viajero hace más de un siglo, un referente fundamental de las

  15. Preventive conservation and management: contribution to a new integrated model, based on the case study of National Archive Torre do Tombo, Portugal

    Directory of Open Access Journals (Sweden)

    Luís Filipe Raposo Pereira

    2016-01-01

    Full Text Available The development of a management model, in the preventive conservation area in the National Archive Torre do Tombo, included the identification of the specificities related to assessment and risk management methodology in Archives area/field and the definition of concepts and processes connected to management in that context. The present paper will focus on those contributions, particularly in the changes resulting by a new perspective in terms of management, based in the methodology defined by the AS/NZS 4360 standard. It also represents the end of the characterization of a process with two sequential phases, corresponding to the periods 2006-2007 and 2009-2013, which intention was to reinforce management assumptions in the preventive conservation field.

  16. Preventive conservation and management: contribution to a new integrated model, based on the case study of National Archive Torre do Tombo, Portugal

    Directory of Open Access Journals (Sweden)

    Luís Filipe Raposo Pereira

    2016-01-01

    Full Text Available The development of a management model, in the preventive conservation area in the National Archive Torre do Tombo, included the identification of the specificities related to assessment and risk management methodology in Archives area/field and the definition of concepts and processes connected to management in that context. The present paper will focus on those contributions, particularly in the changes resulting by a new perspective in terms of management, based in the methodology defined by the AS/NZS 4360 standard. It also represents the end of the characterization of a process with two sequential phases, corresponding to the periods 2006-2007 and 2009-2013, which intention was to reinforce management assumptions in the preventive conservation field.

  17. 大堡礁和托雷斯海峡航路新变化%Latest Changes in Great Barrier Reef and Torres Strait Navigation

    Institute of Scientific and Technical Information of China (English)

    许永强

    2007-01-01

    大堡礁(Great Barrier Reef)位于澳大利亚东北部昆士兰州海岸外,从北部的Cape York(1041S 14232E),向东南延伸到Lady Elliot Island(2407S 15243E),绵延约两千多公里,是世界上最大的珊瑚礁群。托雷斯海峡(TorresStrait)位于约克角半岛和巴布亚新几内亚之间.连接珊瑚海和阿拉弗拉海。由于地理条件的特殊性,

  18. Torres, Anália Cardoso (org., Francisco Vieira da Silva, Teresa Líbano Monteiro, Miguel Cabrita, Homens e mulheres entre família e trabalho

    Directory of Open Access Journals (Sweden)

    Mónica Lopes

    2012-10-01

    Full Text Available Homens e mulheres entre família e trabalho é um trabalho coordenado por Anália Cardoso Torres, uma autora que é uma importante referência no âmbito dos estudos sobre a família, e em particular sobre a conjugalidade. Esta obra, cuja base empírica mais importante é um inquérito por ques­tionário aplicado em 1999, procura anali­sar as diferentes dimensões da divisão se­xual e familiar do trabalho pago e não pago, abrindo caminho à melhor com­preensão das práticas que neste plano têm lugar no ter...

  19. Patrimonio histórico y metaversos. Estudio de caso de la recreación interactiva de la Torre de Hércules en Second Life.

    Directory of Open Access Journals (Sweden)

    Viviana Barneche Naya

    2010-05-01

    Full Text Available El potencial de los metaversos, mundos virtuales tridimensionales multiusuario on-line, donde los usuarios crean sus contenidos, que abarcan campos tan diversos como la cultura, la ciencia, la educación o la recreación patrimonial entre otras, va mas allá de actuar como generador de conexiones humanas y de alojar actividades sociales compartidas, abren la posibilidad de compartir experiencias vivenciales en un mismo “lugar físico virtual”. Este artículo describe la experiencia de los autores en la reconstrucción virtual interactiva de la emblemática Torre de Hércules de A Coruña en Second Life, realizada con fines divulgativos, incidiendo en la problemática encontrada y las soluciones técnicas empleadas para su realización.

  20. Las casas-torre bajomedievales. Análisis sistémico de un proceso de reestructuración espacial/territorial

    Directory of Open Access Journals (Sweden)

    Azkarate Garai-Olaun, Agustín

    2004-12-01

    Full Text Available The torre banderiza is one of the most relevant elements of Basque building heritage., Representative examples of this type of architecture can be found throughout the autonomous community, characteristic of a turbulent period which lasted a little longer than the low medieval crisis (14th to 16th centuries. This study sets out to formulate a systemic, interpretative and integrating proposal, based on stratigraphic stonework analysis, to show the specific relevance of this construction as an element of administering resources in the territory. But above all the work attempts to understand historical-archaeological research.La torre banderiza es uno de los más relevantes elementos del patrimonio construido vasco. Diseminados por toda la geografía de la comunidad autónoma, se pueden encontrar ejemplos representativos de este tipo arquitectónico, característico de una convulsa etapa que se prolongó algo más allá del período de crisis bajomedieval (siglos XIV-XVI. Este trabajo pretende por un lado formular una propuesta interpretativa sistémica e integradora que, partiendo del análisis estratigráfico de las fábricas, muestre el peso específico de esta construcción como elemento de gestión de los recursos del territorio. Pero sobre todo, en las siguientes líneas se hace una decidida apuesta por una forma de entender la investigación histórico-arqueológica.

  1. La adaptación de la forma a las cargas en el arte estructural: desde las pilas y el arco, a la torre

    Directory of Open Access Journals (Sweden)

    Serrano-López, R.

    2014-06-01

    Full Text Available The shape of the Eiffel Tower has been discussed several times, seeking for physical and mathematical relationships. This article aims to address this analysis from a different point of view: the historical and sequential evolution of design over the course of the Eiffel company. Thus, the relationship between the experience gained in different works and technicians can be verified. The paper also tries to give merit to work of all the collaborators of Eiffel over those years. In short, the final solution of the Tower is contrasted and corroborated as a great example of structural art, as has been described in the past.La forma de la Torre Eiffel ha sido varias veces analizada, buscando relaciones físicas y matemáticas. Este artículo pretende abordar este análisis desde un punto de vista diferente, la evolución histórica y secuencial del diseño a lo largo de la trayectoria de la empresa de Eiffel. De esta manera, puede comprobarse la relación entre la experiencia acumulada en diferentes obras y técnicos. Se intenta además atribuir los méritos que corresponden a todos los colaboradores de Eiffel a lo largo de esos años. En definitiva, se contrasta y corrobora de esta manera a la solución final de la Torre como un magnífico ejemplo de arte estructural, como ya ha sido descrito en otras ocasiones.

  2. Simulation of a non-equilibrium helium plasma bullet emerging into oxygen at high pressure (250-760 Torr) and interacting with a substrate

    Science.gov (United States)

    Yan, Wen; Economou, Demetre J.

    2016-09-01

    A two-dimensional computational study of a plasma bullet emanating from a helium gas jet in oxygen ambient at high pressure (250-760 Torr) was performed, with emphasis on the bullet interaction with a substrate. Power was applied in the form of a trapezoidal +5 kV pulse lasting 150 ns. A neutral gas transport model was employed to predict the concentration distributions of helium and oxygen in the system. These were then used in a plasma dynamics model to investigate the characteristics of the plasma bullet during its propagation and interaction with a substrate. Upon ignition, the discharge first propagated as a surface wave along the inner wall of the containing tube, and then exited the tube with a well-defined ionization front (streamer or plasma bullet). The plasma bullet evolved from a hollow (donut-shaped) feature to one where the maximum of ionization was on axis. The bullet propagated in the gap between the tube exit and the substrate with an average speed of ˜2 × 105 m/s. Upon encountering a metal substrate, the bullet formed a conductive channel to the substrate. Upon encountering a dielectric substrate, the bullet turned into an ionization wave propagating radially along the substrate surface. For a conductive substrate, the radial species fluxes to the surface peaked on the symmetry axis. For a dielectric substrate, a ring-shaped flux distribution was observed. The "footprint" of plasma-surface interaction increased either by decreasing the gap between tube exit and substrate, decreasing the relative permittivity of an insulating substrate, or decreasing pressure. As the system pressure was lowered from 760 to 250 Torr, the discharge was initiated earlier, and the plasma bullet propagation speed increased. A reverse electric field developed during the late stages of the ramp-down of the pulse, which accelerated electrons forming a brief backward discharge.

  3. DNA-based identifications reveal multiple introductions of the vegetable leafminer Liriomyza sativae (Diptera: Agromyzidae) into the Torres Strait Islands and Papua New Guinea.

    Science.gov (United States)

    Blacket, M J; Rice, A D; Semeraro, L; Malipatil, M B

    2015-10-01

    Leafmining flies (Diptera: Agromyzidae) can be serious economic pests of horticultural crops. Some genera such as Liriomyza are particularly problematic with numerous species, some of which are highly polyphagous (wide host range), which can only be confidently identified morphologically from adult males. In our study, DNA barcoding was employed to establish new locality records of the vegetable leafminer fly, Liriomyza sativae, from the islands of Torres Strait (Queensland, Australia) and the central highlands of Papua New Guinea (PNG). These records represent significant range extensions of this highly invasive plant pest. Specimens of immature leafminers (from leaf mines) were collected over a 5-year period during routine plant health surveys in ethanol or on FTA® filter paper cards, both methods proved effective at preserving and transporting insect DNA under tropical conditions, with FTA cards possessing some additional logistical benefits. Specimens were identified through sequencing two sections of the cytochrome oxidase I gene and the utility of each was assessed for the identification of species and intra-specific genetic lineages. Our study indicates that multiple haplotypes of L. sativae occur in PNG, while a different haplotype is present in the Torres Strait, with genetic regionalization between these areas apart from a single possible instance - one haplotype 'S.7' appears to be common between these two regions - interestingly this has also been the most common haplotype detected in previous studies of invasive L. sativae populations. The DNA barcoding methods employed here not only identified multiple introductions of L. sativae, but also appear generally applicable to the identification of other agromyzid leafminers (Phytomyzinae and Agromyzinae) and should decrease the likelihood of potentially co-amplifying internal hymenopteran parasitoids. Currently, L. sativae is still not recorded from the Australian mainland; however, further sampling of

  4. 苦参碱对人肾母细胞瘤SK-NEP-1细胞趋化因子受体4表达的影响%The effect of matrine on CXCR4 expression in SK-NEP-1 cells

    Institute of Scientific and Technical Information of China (English)

    毛玲; 薛天阳; 许伟

    2014-01-01

    Objectives To investigate the effects of matrine on the proliferation and apoptosis of SK-NEP-1 cells in vitro, and its possible mechanism. Methods Trials were divided into following groups:control group, 0.5, 1.0 and 1.5mg/ml of ma-trine intervention groups. The inhibition rate of SK-NEP-1 cells treated with different concentration of Matrine was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry (FCM). RT-PCR analysis was employed to measure the PDCD4 mRNA expression. Results Matrine (final concentrations=0.5, 1.0 and 1.5mg/ml) could induce apoptosis and inhibit the growth of SK-NEP-1 cells. Compared with the controls without matrine treatment (8.81±3.71)%, the inhibition rates of SK-NEP-1 cells were (20.79 ± 6.20)%, (31.25 ± 5.07)%, and (51.15 ± 12.70)%, respectively;the apoptotic rates of SK-NEP-1 cells treated with different concentration of matrine were (13.67±0.78)%,(17.43±1.65)%and (20.80±1.54)%, respectively. Significant difference in the inhibition and apoptotic rates of SK-NEP-1 cells between each drug group and control group was observed(P<0.05), and the inhibition and apoptotic rates of SK-NEP-1 cells increased gradually with increased matrine concentration, thus exhibiting a dose-dependent effect(P<0.05). To the expression of CXCR4 mRNA,the grey levels of SK-NEP-1 cells treated with matrine intervention group (final concentrations=0.5, 1.0 and 1.5 mg/ml) were (0.720 ± 0.058), (0.540 ± 0.095) and (0.307 ± 0.050), respectively. The mRNA expression of CXCR4 was seen in SK-NEP-1 cells. Compared with control group, the expres-sion of CXCR4 mRNA was decreased significantly in matrine intervention group (P<0.01).There were significant difference in CXCR4 mRNA level among the SK-NEP-1 cells treated with 0.5,1.0,1.5mg/mL of matrine (P<0.01). Conclusions Matrine could induce apoptosis and inhibit the growth of SK-NEP-1 cells in a dose-dependent way which may be associated with the down-regulated CXCR4 expression in SK

  5. Inhibition of beta-site amyloid precursor protein-cleaving enzyme and beta-amyloid precursor protein genes in SK-N-SH cells

    Institute of Scientific and Technical Information of China (English)

    Suqin Gao; Lin Sun; Enji Han; Hongshun Qi; Jinbo Feng; Shunliang Xu; Wen Xia

    2009-01-01

    BACKGROUND:Previous studies have demonstrated that Piper futokadsura stem selectively inhibits expression of amyloid precursor protein (APP) at the mRNA level.In addition,the piperlonguminine (A) and dihydropiperlonguminine (B) components (1:0.8),which can be separated from Futokadsura stem,selectively inhibit expression of the APP at mRNA and protein levels.OBJECTIVE:Based on previous findings,the present study investigated the effects of β-site amyloid precursor protein cleaving enzyme (BACE1) and APP genes on the production of β-amyloid peptide 42 (Aβ42) in human neuroblastoma cells (SK-N-SH cells) using small interfering RNAs (siRNAs) and A/B components separated from Futokadsura stem,respectively.DESIGN,TIME AND SETTING:A gene interference-based randomized,controlled,in vitro experiment was performed at the Key Laboratory of Cardiovascular Remodeling and Function Research,Ministries of Education and Public Health,and Institute of Pharmacologic Research,School of Pharmaceutical Science & Department of Biochemistry,School of Medicine,Shandong University between July 2006 and December 2007.MATERIALS:SK-N-SH cells were provided by Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai,China;mouse anti-human BACE1 monoclonal antibody was purchased from R&D Systems,USA;mouse anti-human APP monoclonal antibody was purchased from Cell Signaling Technology,USA;and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG was provided by Sigma,USA.METHODS:The human BACE1 cDNA sequence was obtained from NCBI website (www.ncbi.nlm.nih.gov/sites/entrez).Three pairs of siRNAs,specific to human BACE1 gene,were synthesized through the use of Silencer? pre-designed siRNA specification,and were transfected into SK-N-SH cells with siPORT NeoFX transfection agent to compare the effects of different concentrations of siRNAs (10-50 nmol/L) on SK-N-SH cells.Futokadsura stem was separated and purified with chemical methods,and the crystal was composed of

  6. 噬菌体肽库筛选与SK-BR-3细胞表面HER2特异性结合的活性短肽%Screening Short Active Peptide Combinded with HER2 on SK-BR-3 by Phage Display Peptide Library

    Institute of Scientific and Technical Information of China (English)

    汪静; 高晓娟; 吴秀丽

    2015-01-01

    目的 利用噬菌体展示肽库技术筛选与人乳腺癌细胞表面HER2特异性结合的活性短肽.方法 以过表达HER2的人乳腺癌细胞SK-BR-3为靶细胞,Herceptin(R)作为竞争性洗脱剂,采用竞争结合实验,经四轮亲和筛选,获得阳性噬菌体富集;通过ELISA评价与SK-BR-3细胞高特异性结合的阳性噬菌体克隆;分析DNA测序结果并推导短肽序列;采用MTT法比较筛选短肽与Herceptin(R)对SK-BR-3细胞生长的抑制率.结果 随机挑选的16个克隆对SK-BR-3细胞具有特异性结合力,其中S-1阳性克隆与靶细胞特异结合活性最强,且其短肽序列对SK-BR-3细胞生长具有一定的抑制作用.结论 通过噬菌体展示肽库对过表达HER2乳腺癌细胞进行筛选,获得与SK-BR-3细胞高特异性结合的活性短肽序列,为设计乳腺癌靶向药物与导向治疗提供参考数据.

  7. Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation

    Directory of Open Access Journals (Sweden)

    Anett Illing

    2013-01-01

    Full Text Available Pluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the expression and the role of ion channels during human embryogenesis, organ development, and cell fate determination. Of note, small and intermediate conductance, calcium-activated potassium channels have been reported to modify stem cell behaviour and differentiation. These channels are broadly expressed throughout human tissues and are involved in various cellular processes, such as the after-hyperpolarization in excitable cells, and also in differentiation processes. To this end, human induced pluripotent stem cells (hiPSCs generated from plucked human hair keratinocytes have been exploited in vitro to recapitulate endoderm formation and, concomitantly, used to map the expression of the SK channel (SKCa subtypes over time. Thus, we report the successful generation of definitive endoderm from hiPSCs of ectodermal origin using a highly reproducible and robust differentiation system. Furthermore, we provide the first evidence that SKCas subtypes are dynamically regulated in the transition from a pluripotent stem cell to a more lineage restricted, endodermal progeny.

  8. Sprawozdanie z polityki rachunkowości jako odrębny składnik sprawozdania finansowego

    Directory of Open Access Journals (Sweden)

    Ewa Walińska

    2016-07-01

    Full Text Available Niniejszy artykuł poświęcono rozważaniom na temat polityki rachunkowości z perspektywy użytkownika sprawozdania finansowego. Jego celem jest prezentacja nowej formuły ujawnień informacji dotyczącej polityki rachunkowości, która zdaniem autorki podnosiłaby rangę i znaczenie tego elementu systemu rachunkowości. W artykule przedstawiono istotę i miejsce polityki rachunkowości w sprawozdaniu finansowym w świetle obowiązującego prawa bilansowego i praktyki największych spółek publicznych notowanych na Giełdzie Papierów Wartościowych w Warszawie. Podsumowaniem rozważań jest propo- zycja ujawniania polityki rachunkowości jako odrębnego składnika sprawozdania finansowego – spra- wozdania z polityki rachunkowości.

  9. From laboratory to pilot plant: the solid-state process development of a highly potent cathepsin S/K inhibitor.

    Science.gov (United States)

    Feth, Martin Philipp; Heyse, Winfried; Baumgartner, Bruno; Nagel, Norbert; Tappertzhofen, Christoph; Olpp, Thomas; Jurascheck, Jörg; Ulrich, Joachim; Helmdach, Lydia; Petzoldt, Christine

    2013-04-01

    The solid-state development for the low dose drug molecule SAR114137, a selective and reversible inhibitor of cysteine cathepsin S/K, is reported. Six polymorphic forms as well as various solvate phases were discovered by an extensive polymorphism screening. The solid phase characterizations revealed that phase 1, from which a single crystal structure could be obtained, is the thermodynamically most stable form and therefore it was chosen for pharmaceutical development. The successful scale-up from development laboratory into pilot plant for the crystallization and drying processes is presented. Testing of different drying techniques, like agitated drying in conical or filter dryers as well as spray drying, proved them to be very promising alternatives to the conventional tray drying process and might be used during the industrialization phase of the project. The use of online analytical tools (e.g., Raman spectroscopy) for a better process understanding and as tools for process optimization is shown. Furthermore, wet milling by ultrasound was performed on laboratory scale and discussed as potential option to reach the desired particle size distribution necessary for a good content uniformity of the API in an oral formulation.

  10. Emissions from the copper-nickel industry on the Kola Peninsula and at Noril'sk, Russia

    Science.gov (United States)

    Boyd, Ron; Barnes, S.-J.; De Caritat, P.; Chekushin, V.A.; Melezhik, V.A.; Reimann, C.; Zientek, M.L.

    2009-01-01

    Published estimates for base metal emissions from the copper-nickel industry on the Kola Peninsula are re-examined in the light of (a) chemical data on the composition of the ores; (b) official emission figures for 1994; and (c) modelled emissions based on dry and wet deposition estimates derived from data for snow and rain samples collected in 1994. The modelled emissions, official emission figures and chemical data are mutually compatible for Ni, Cu and Co and show that previously published figures underestimated the emissions of the major elements, Ni and Cu (though within the same order of magnitude) and overestimated the emissions of As, Pb, Sb and Zn by up to several orders of magnitude, in some cases exceeding the calculated total input to the plants. Published estimates have neglected information on the nature and chemistry of the ores processed in metallurgical industries in the Noril'sk area of Siberia and the Urals. Revised emission estimates for 1994, using knowledge of the chemistry of the ores, are proposed: taken with published information on total emissions up to 2000 these data give an indication of emission levels in more recent years. ?? 2008 Elsevier Ltd. All rights reserved.

  11. Spectroscopic and Gas Chromatographic Studies of Pigments and Binders in Gdańsk Paintings of the 17th Century

    Directory of Open Access Journals (Sweden)

    Justyna Olszewska-Świetlik

    2013-01-01

    Full Text Available This work presents spectroscopic (optical microscopy, OM; micro-Raman, Raman; and Fourier-transform adsorption infrared, FT-IR and gas chromatographic studies of two famous panel paintings from the Gdańsk artists of the 17th century Golden Age, “Servilius Appius” by Isaac van den Blocke and “Allegory of Wealth” probably by Anton Möller. Application of the aforementioned methods allowed us to identify pigments and binders used in the panel paintings. In particular, it was determined that the yellow pigment used by both artists is lead-tin yellow type I (2PbO·SnO2, the white pigment is lead (II carbonate hydroxide (2PbCO3·Pb(OH2, and the ground layer material consist of chalk (CaCO3. The analysis showed also that in the case of “Allegory of Wealth,” the red layer consists not only of cinnabar (HgS but also of lead-tin yellow type I.

  12. Contamination of Runoff Water at Gdańsk Airport (Poland by Polycyclic Aromatic Hydrocarbons (PAHs and Polychlorinated Biphenyls (PCBs

    Directory of Open Access Journals (Sweden)

    Jacek Namieśnik

    2011-12-01

    Full Text Available Airport runoff can contain high concentrations of various pollutants, in particular polycyclic aromatic hydrocarbons (PAHs and polychlorinated biphenyls (PCBs, the environmental levels of which have to be monitored. Airport runoff water samples, collected at the Gdańsk-Rębiechowo Airport from 2008 to 2009, were analysed for PAHs and PCBs by gas chromatography. The aromatic fractions were separated by liquid-liquid extraction and analysed by GC/MS. Total PAH concentrations were 295–6,758 ng/L in 2008 and 180–1,924 ng/L in 2009, while total PCB levels in 2008 ranged from 0.14 to 0.44 µg/L and in 2009 from 0.06 to 0.23 µg/L. The PAH and PCB compositions in airport runoff waters were examined over a range of spatial and temporal scales to determine distributions, trends and possible sources. This pollution is mainly pyrolytic and related to anthropogenic activity. There were significant differences between the samples collected in the two seasons. An understanding of the magnitude of contamination due to airport runoff water is important for the effective management of airport infrastructure.

  13. Immobilization of α-Amylase from Anoxybacillus sp. SK3-4 on ReliZyme and Immobead Supports

    Directory of Open Access Journals (Sweden)

    Ummirul Mukminin Kahar

    2016-09-01

    Full Text Available α-Amylase from Anoxybacillus sp. SK3-4 (ASKA is a thermostable enzyme that produces a high level of maltose from starches. A truncated ASKA (TASKA variant with improved expression and purification efficiency was characterized in an earlier study. In this work, TASKA was purified and immobilized through covalent attachment on three epoxide (ReliZyme EP403/M, Immobead IB-150P, and Immobead IB-150A and an amino-epoxide (ReliZyme HFA403/M activated supports. Several parameters affecting immobilization were analyzed, including the pH, temperature, and quantity (mg of enzyme added per gram of support. The influence of the carrier surface properties, pore sizes, and lengths of spacer arms (functional groups on biocatalyst performances were studied. Free and immobilized TASKAs were stable at pH 6.0–9.0 and active at pH 8.0. The enzyme showed optimal activity and considerable stability at 60 °C. Immobilized TASKA retained 50% of its initial activity after 5–12 cycles of reuse. Upon degradation of starches and amylose, only immobilized TASKA on ReliZyme HFA403/M has comparable hydrolytic ability with the free enzyme. To the best of our knowledge, this is the first report of an immobilization study of an α-amylase from Anoxybacillus spp. and the first report of α-amylase immobilization using ReliZyme and Immobeads as supports.

  14. Reproduktorová skříň pro laboratorní měření impedance reproduktoru

    OpenAIRE

    Nevole, Tomáš

    2009-01-01

    V této práci je řešena problematika návrhu a konstrukce univerzální modulární reproduktorové skříně- ozvučnice, která by měla sloužit pro laboratorní měření impedance širokopásmového elektromagnetického reproduktoru. Toto měření má prokázat, jak se mění elektrické parametry reproduktoru vlivem změny vlastností akustického prostředí, do nějž vyzařuje. V první části je nejprve vysvětlena potřebná teorie ke zvládnutí problému, jako způsob šíření zvukového vlnění látkovým prostředím, vlastnosti a...

  15. Neuroprotective effects of KR-62980, a new PPARγ agonist, against chemical ischemia-reperfusion in SK-N-SH cells.

    Science.gov (United States)

    Kim, Ki Young; Cho, Hyun Sill; Lee, Su Hee; Ahn, Jin Hee; Cheon, Hyae Gyeong

    2011-02-01

    PPARγ agonists exert neuroprotective effects against various types of brain injuries. In the present study, we investigated the effects of KR-62980, a new PPARγ agonist, and rosiglitazone on the neuronal cell death induced by chemical ischemia-reperfusion in SK-N-SH cells and their underlying molecular mechanisms. Both agonists inhibited chemical ischemia-reperfusion-induced cell death, and the effects were associated with anti-apoptotic action. KR-62980 and rosiglitazone suppressed NO and ROS formation, and N-acetyl-N-acetoxy-4-chlorobenzenesulfonamide, an NO generator, reversed the protective effects of the agonists on cell viability. In the agonist-induced anti-apoptotic process, PTEN expression was suppressed in parallel with increased Akt and ERK phosphorylation, whereas PD98059 (an ERK inhibitor) or wortmannin (a PI-3K inhibitor) abolished the cell survival by KR-62980 and rosiglitazone. All of the effects of KR-62980 and rosiglitazone appeared to be PPARγ-dependent because the effects were reversed by bisphenol A diglycidyl ether, a PPARγ antagonist, or by PPARγ knockdown. Our results demonstrate that two PPARγ agonists, KR-62980 and rosiglitazone, inhibited chemical ischemia-reperfusion-induced neuronal cell death by PPARγ-mediated anti-apoptotic and anti-oxidant mechanisms related to PTEN suppression and ERK phosphorylation.

  16. A synergistic antiproliferation effect of curcumin and docosahexaenoic acid in SK-BR-3 breast cancer cells: unique signaling not explained by the effects of either compound alone

    Directory of Open Access Journals (Sweden)

    Jo Davisson V

    2011-04-01

    Full Text Available Abstract Background Breast cancer is a collection of diseases in which molecular phenotypes can act as both indicators and mediators of therapeutic strategy. Therefore, candidate therapeutics must be assessed in the context of multiple cell lines with known molecular phenotypes. Docosahexaenoic acid (DHA and curcumin (CCM are dietary compounds known to antagonize breast cancer cell proliferation. We report that these compounds in combination exert a variable antiproliferative effect across multiple breast cell lines, which is synergistic in SK-BR-3 cells and triggers cell signaling events not predicted by the activity of either compound alone. Methods Dose response curves for CCM and DHA were generated for five breast cell lines. Effects of the DHA+ CCM combination on cell proliferation were evaluated using varying concentrations, at a fixed ratio, of CCM and DHA based on their individual ED50. Detection of synergy was performed using nonlinear regression of a sigmoid dose response model and Combination Index approaches. Cell molecular network responses were investigated through whole genome microarray analysis of transcript level changes. Gene expression results were validated by RT-PCR, and western blot analysis was performed for potential signaling mediators. Cellular curcumin uptake, with and without DHA, was analyzed via flow cytometry and HPLC. Results CCM+DHA had an antiproliferative effect in SK-BR-3, MDA-MB-231, MDA-MB-361, MCF7 and MCF10AT cells. The effect was synergistic for SK-BR-3 (ER- PR- Her2+ relative to the two compounds individually. A whole genome microarray approach was used to investigate changes in gene expression for the synergistic effects of CCM+DHA in SK-BR-3 cells lines. CCM+DHA triggered transcript-level responses, in disease-relevant functional categories, that were largely non-overlapping with changes caused by CCM or DHA individually. Genes involved in cell cycle arrest, apoptosis, inhibition of metastasis, and

  17. Study on geometric approach of SVM algorithm SK algorithm analysis and study%SVM的几何方法——SK类思路的研究

    Institute of Scientific and Technical Information of China (English)

    常振华; 陈伯成; 李英杰; 刘文煌; 闫学为

    2011-01-01

    The geometric approach of the Support Vector Machine(SVM) is a kind of geometric way to find the solution to the problem of the SVM algorithm. Based on its geometric characters,the SK(Schlesinger-Kozinec) algorithm is studied intuitively. It briefly sums up the two convex hulls,based on their relative positions, into five categories,and makes sure their optimizing position got in each computing is mostly at the hull vertices or boundary,it can get to the boundary(the optimization place of the computing) at the first computation.The manual single-step simulation results show that the projection is not always successful for such kind of algorithms in many cases,though it can't affect the computing result,but can weaken the algorithm efficiency. Based on the analysis,it demonstrates two improving ways for the soft SK algorithm(Backward-SK and Forward-SK methods),and makes some simulation for comparing. The simulation results show that the improved method computing results are almost same as the SK and soft SK ones,but the computing process of improved one is more intuitive.%支持向量机(Support Vector Machine,SVM)的几何方法是一种基于SVM计算过程中几何意义出发的求解方法.利用其几何特点,比较直观地对其基本算法的构建过程进行了分析.两凸包相对位置可以简要地归纳成5类,且在该类算法迭代过程最优点多在顶点和边界上,该类算法在第一次迭代就可能达到边界(最优点);该类算法的手动单步模拟计结果揭示:很多情况下,该类算法迭代过程的投影并不成功,虽不影响解法的最终结果,但会影响迭代效率:基于几何的分析,给出软SK软算法的两种改进思路(Backward-SK和Forward-SK思路),并进行了仿真比较计算.实验表明,该方法计算效果与原思路相似,但是计算过程理解更加直观.

  18. Metodología e investigación del faro romano en la restauración de la Torre de Hércules de A Coruña (1990-1992)

    OpenAIRE

    Latorre Gonzalez-Moro, Pablo

    2009-01-01

    Propuesta de reconstrucción del faro romano de La Coruña a partir de los restos conservados en el nucleo del faro conocida como La torre de Hércules, los restos de su cimentación puestos al descubierto durante su restauración en 1992 y las fuentes documentales existentes.

  19. Connection of Magnetic Antisense Probe with SK-Br-3 Oncocyte mRNA Nucleotide Detected by High Resolution Atomic Force Microscope%高精度原子力显微镜显示磁性反义探针与SK-Br-3肿瘤细胞mRNA核苷酸连接

    Institute of Scientific and Technical Information of China (English)

    谭书德; 欧阳羽; 李信友; 文明; 李少林

    2011-01-01

    为了将高精度原子力显微镜(AFM)用于显示超顺磁性氧化铁标记的c-erbB2癌基因反义寡脱氧核苷酸探针(磁性反义探针)与SK-Br-3肿瘤细胞mRNA核苷酸的连接,我们在磁性反义探针转染SK-Br-3肿瘤细胞基础上,用AFM对转染后的肿瘤细胞进行观察,并同时对转染后的肿瘤细胞进行蛋白表达检测及MRI成像,以进一步证实AFM的观察结果.从AFM显示的磁性反义探针转染SK-Br-3肿瘤细胞后单个细胞的全貌图及局部放大图发现,探针中反义寡脱氧核苷酸中的脱氧胞嘧啶核苷酸闭环与肿瘤细胞mRNA嘌呤核苷酸环相连接;此外,磁性反义探针能特异性抑制SK-Br3细胞c-erbB2的蛋白表达,MRI显示磁性反义探针转染SK-Br-3肿瘤细胞的信号强度最低(P<0.05).实验表明,AFM可以清楚显示磁性反义探针与SK-Br-3肿瘤细胞核mRNA核苷酸的连接.%The present paper is aimed to detect superparamagnetic iron oxide labeled c-erbB2 oncogene antisense oli-gonucleotide probe (magnetic antisense probe) connected with SK-Br-3 oncocyte mRNA nucleotide by high resolution atomic force microscope (AFM). We transfected SK-Br-3 oncocyte with magnetic antisense probe, then observed the cells by AFM with high resolution and detected protein expression and magnetic resonance imagine ( MRI). The high resolution AFM clearly showed the connection of the oligonucleotide remote end of magnetic antisense probe with the mRNA nucleotide of oncocyte. The expression of c-erbB2 protein in SK-Br3 cells were highly inhibited by using magnetic antisense probe. We then obtained the lowest signal to noise ratio (SNR) of SK-Br-3 oncocyte transfected with magnetic antisense probe by MRI (P<0. 05). These experiments demonstrated that the high resolution AFM could be used to show the binding of magnetic antisense probe and SK-Br-3 mRNA of tumor cell nuclear.

  20. Antiarrhythmic effect of the Ca(2+)-activated K(+) (SK) channel inhibitor ICA combined with either amiodarone or dofetilide in an isolated heart model of atrial fibrillation

    DEFF Research Database (Denmark)

    Kirchhoff, Jeppe Egedal; Diness, Jonas Goldin; Abildgaard, Lea;

    2016-01-01

    whether a combination of the SK channel blocker N-(pyridin-2-yl)-4-(pyridin-2-yl)thiazol-2-amine (ICA) together with either dofetilide or amiodarone provided a synergistic effect. The duration of AF was reduced with otherwise subefficacious concentrations of either dofetilide or amiodarone when combined......Dose is an important parameter in terms of both efficacy and adverse effects in pharmacological treatment of atrial fibrillation (AF). Both of the class III antiarrhythmics dofetilide and amiodarone have documented anti-AF effects. While dofetilide has dose-related ventricular side effects......, amiodarone primarily has adverse non-cardiac effects. Pharmacological inhibition of small conductance Ca(2+)-activated K(+) (SK) channels has recently been reported to be antiarrhythmic in a number of animal AF models. In a Langendorff model of acutely induced AF on guinea pig hearts, it was investigated...

  1. Pharmacologic inhibition of small-conductance calcium-activated potassium (SK) channels by NS8593 reveals atrial antiarrhythmic potential in horses

    DEFF Research Database (Denmark)

    Haugaard, Maria Mathilde; Hesselkilde, Eva Zander; Pehrson, Steen Michael

    2015-01-01

    . The electrophysiologic and anti-AF effects of the relative selective SK channel inhibitor NS8593 (5 mg/kg IV) were evaluated in anesthetized horses, focusing on the potential of NS8593 to terminate acute pacing-induced AF, drug-induced changes in atrial effective refractory period, AF duration and vulnerability....... NS8593 terminated all induced AF episodes (duration ≥15 minutes), caused pronounced prolongation of atrial effective refractory period, and reduced AF duration and vulnerability. QRS duration and QTc interval were not affected by treatment. CONCLUSION: SK channels are widely distributed in atrial...... and ventricular cardiomyocytes and contribute to atrial repolarization. Inhibition by NS8593 terminates pacing-induced AF of short duration and decreases AF duration and vulnerability without affecting ventricular conduction and repolarization. Thus, inhibition by NS8593 demonstrates clear atrial antiarrhythmic...

  2. The Skælskør structure in eastern Denmark – wrench-related anticline or primary Late Cretaceous sea-floor topography?

    DEFF Research Database (Denmark)

    Surlyk, Finn; Boldreel, Lars Ole; Lykke-Andersen, Holger;

    2010-01-01

    Sorgenfrei (1951) identified a number of NW–SE oriented highs in the Upper Cretaceous – Danian Chalk Group in eastern Denmark, including the Skælskør structure and interpreted them as anticlinal folds formed by wrenching along what today is known as the Ringkøbing-Fyn High. Recent reflection...... seismic studies of the Chalk Group in Øresund and Kattegat have shown that similar highs actually represent topographic highs on the Late Cretaceous – Danian seafloor formed by strong contourparallel bottom currents. Reflection seismic data collected over the Skælskør structure in order to test...... the hypothesis of Sorgenfrei show that the Base Chalk reflection is relatively flat with only very minor changes in inclination and cut by only a few minor faults. The structure is situated along the northern margin of a high with roots in a narrow basement block, projecting towards the northwest from...

  3. Współistnienie linijnej IgA dermatozy pęcherzowej z atopowym zapaleniem skóry. Trudności diagnostyczne. Opis przypadku

    Directory of Open Access Journals (Sweden)

    Marcin Zakrzewski

    2011-12-01

    Full Text Available Wprowadzenie: Linijna IgA dermatoza pęcherzowa (ang. linear IgAbullous dermatosis – LABD jest przewlekłą autoimmunologiczną chorobąpęcherzową skóry, która niezwykle rzadko współistnieje z innymischorzeniami. Cel pracy: Przedstawienie wyjątkowo rzadkiego współistnienia LABDz atopowym zapaleniem skóry (AZS. Opis przypadku: Kobieta, lat 28, zgłosiła się do poradni dermatologicznejz powodu rozsianych na skórze całego ciała, układających sięobrączkowato zmian pęcherzowych na rumieniowym podłożu oraznadżerek w obrębie błon śluzowych jamy ustnej. Poza tym na skórzetwarzy, nadgarstków oraz w zgięciach łokciowych stwierdzano zmianytypowe dla AZS. Pacjentka od wczesnego dzieciństwa choruje naAZS, idiopatyczną nadpłytkowość oraz obecnie na bezobjawowewrzodziejące zapalenie jelita grubego. U matki kobiety rozpoznanostwardnienie rozsiane. W badaniu immunofluorescencyjnym bezpośrednimwycinka skóry wykazano obecność linijnych złogów IgA nagranicy skórno-naskórkowej, za pomocą badania immunofluorescencyjnegopośredniego nie stwierdzono obecności przeciwciał reagującychz błoną podstawną, a metodą ELISA przeciwciał anty-NC16A. Napodstawie obrazu klinicznego oraz wykonanych badań rozpoznanoLABD, co dodatkowo potwierdzono badaniem wycinka skóry metodąmikroskopii konfokalnej. Ze względu na nadpłytkowość pacjentkabyła konsultowana hematologicznie i miała wykonaną biopsję szpiku.Rozpoznano u niej nadpłytkowość idiopatyczną. Pomimo zaburzeńhematologicznych podjęto leczenie prednizonem w dawkach zmniejszającychsię (od 40 mg/dobę oraz enoksaparyną. Podczas terapiiobserwowano systematyczną poprawę kliniczną, pęcherze wchłonęłysię, pozostawiając pozapalne przebarwienia. Przy redukcji dawki prednizonuobserwowano stopniowe pogarszanie się zmian skórnych typowychdla AZS, zwłaszcza w obrębie twarzy i kończyn górnych. Z tegowzględu włączono leczenie cyklosporyną A. Wnioski

  4. Epibionts and parasites on crustaceans (Copepoda, Cladocera, Cirripedia larvae inhabiting the Gulf of Gdańsk (Baltic Sea in very large numbers

    Directory of Open Access Journals (Sweden)

    Luiza Bielecka

    2014-06-01

    Full Text Available The occurrence of epizoic filter-feeding Protozoa (Vorticella and Zoothamnium and parasitic Protozoa (Ellobiopsis on Calanoida was noticed in the Gulf of Gdańsk in 1998, 1999 and 2006. The relatively high (4-16% of all calanoids level of infestation varied depending on the type of infestation (0.1-13% of the population of particular taxa. The dominant copepods – Acartia spp., Temora longicornis and Centropages hamatus - were attacked the most frequently (from 10.5% to 54% of all infested calanoids. Epibiosis and parasitism were observed on all copepod developmental stages (adults, juveniles and nauplii. Epibionts and parasites were located on different parts of the body, but mainly on the prosome. Infestation by epibionts and parasites was not restricted to calanoid copepods: it was also detected in non-negligible numbers on other crustaceans, namely, Harpacticoida, Cladocera (Bosmina sp. and Cirripedia larvae (nauplii in the Gulf of Gdańsk.

  5. En dag med vin och historia : Utveckling av en ny produkt för Finlands Skärgårdsbokning

    OpenAIRE

    Salminen, Helen

    2013-01-01

    Dagens resenärer är allt mera intresserade av lokal producerad vin och mat. Under 1990-talet växte enoturismen fram som en nisch och efter alkohollagförändringen 1995 kunde enoturismen också slå rot i Finland. Idag finns det 25 vingårdar runtom i Finland, majoriteten av dessa ligger i södra Finland. Syftet med detta examensarbete är att genom tjänsteutveckling skapa en ny produkt för Finlands Skärgårdsbokning där jag sammanbinder Åbolands Skärgård och enoturismen. För att kunna utveckla denna...

  6. Measuring the CP asymmetry in the decay $D^0 \\rightarrow K^0_SK^0_S$ using Run II data at LHCb.

    CERN Document Server

    Fischer, Kamil Leszek

    2017-01-01

    A sensitivity study for the measurement of $CP$ asymmetry in the decay $D^0 \\rightarrow K^0_{S}K^0_{S}$ has been performed using $pp$ collision data collected by the LHCb experiment during Run II at center-of-mass energy $\\sqrt{s} =13 \\ TeV$. The results indicate that an improvement of a factor of at least three in efficiency is necessary to achieve a world's best precision on $\\mathcal{A}_{CP}$. A proposed set of changes to the trigger selection for this channel is presented. If implemented these changes would increase the signal yield per unit of integrated luminosity by a factor of six, and therefore place LHCb in the position to perform the world's most precise measurement of $\\mathcal{A}_{CP}$($D^0 \\rightarrow K^0_{S}K^0_{S}$).

  7. Synergistic antiarrhythmic effect of combining inhibition of Ca(2+)-activated K(+) (SK) channels and voltage-gated Na(+) channels in an isolated heart model of atrial fibrillation

    DEFF Research Database (Denmark)

    Kirchhoff, Jeppe Egedal; Goldin Diness, Jonas; Sheykhzade, Majid

    2015-01-01

    be subefficacious as monotherapy, may prevent atrial fibrillation (AF) and have reduced proarrhythmic potential in the ventricles. METHODS: Subefficacious concentrations of ranolazine, flecainide, and lidocaine were tested alone or in combination with the SK channel blocker N-(pyridin-2-yl)-4-(pyridin-2-yl...... of the adverse effect profile could be an additional advantage if compound concentrations could be reduced. OBJECTIVE: The purpose of this study was to test the hypothesis that combined inhibition of Ca(2+)-activated K(+) channels (SK channels) and voltage-gated Na(+) channels, in concentrations that would......)thiazol-2-amine (ICA) in a Langendorff-perfused guinea pig heart model in which AF was induced after acetylcholine application and burst pacing. RESULTS: AF duration was reduced when both flecainide and ranolazine were combined with ICA in doses that did not reduce AF as monotherapy. At higher...

  8. Antiarrhythmic effect of the Ca(2+)-activated K(+) (SK) channel inhibitor ICA combined with either amiodarone or dofetilide in an isolated heart model of atrial fibrillation

    DEFF Research Database (Denmark)

    Kirchhoff, Jeppe Egedal; Diness, Jonas Goldin; Abildgaard, Lea;

    2016-01-01

    Dose is an important parameter in terms of both efficacy and adverse effects in pharmacological treatment of atrial fibrillation (AF). Both of the class III antiarrhythmics dofetilide and amiodarone have documented anti-AF effects. While dofetilide has dose-related ventricular side effects...... that combination of subefficacious concentrations of an SK channel blocker and either dofetilide or amiodarone can maintain anti-AF properties, while the risk of ventricular arrhythmias is reduced....

  9. [Oxidative stress and autophagy in SK-N-SH cells induced by manganese chloride or 1-methyl-4-phenylpyridinium: a comparative analysis].

    Science.gov (United States)

    Liu, W L; Dou, C S; Wang, Y; Zhao, P; Fu, J L; Yao, B Y; Zhou, Z C

    2017-02-20

    Objective: To investigate the effect of manganese chloride (MnCl(2)) or 1-methyl-4-phenylpyridinium (MPP (+)) on oxidative stress and autophagy in human neuroblastomaSK-N-SH cells and the mechanism of the neurotoxicity of manganese. Methods: SK-N-SH cells were treated with MnCl(2) or MPP(+) at doses of 0.062 5, 0.125, 0.25, 0.5, 1.0, and 2.0 mmol/L for 24 hours, and MTT assay was used to measure cell viability. The cells weretreated with MnCl(2) or MPP(+) at doses of 0.125, 0.25, and 0.5 mmol/L for 24 hours, and flow cytometry was used to measure the content of reactive oxygen species (ROS) in cells, a laser scanning confocal microscope was used to observe autophagosome in cells, and Western blot was used to measure the expression of autophagy-related proteins P62 and LC3-II/LC3-I. Results: Compared with the control group, the 0.0625-2.0 mmol/L MnCl(2) and 0.125-2.0 mmol/L MPP (+) treatment groups had significant reductions in the viability of SK-N-SH cells, and the 0.25-2.0 mmol/L MnCl(2) treatment groups had significantly lower viability than the groups treated with the same doses of MPP(+) (all PN-SH cells, and MPP(+) has a significantly greater inductive effect on autophagy of SK-N-SH cells than MnCl(2).

  10. Bromodomain and extra-terminal (BET) bromodomain inhibition activate transcription via transient release of positive transcription elongation factor b (P-TEFb) from 7SK small nuclear ribonucleoprotein.

    Science.gov (United States)

    Bartholomeeusen, Koen; Xiang, Yanhui; Fujinaga, Koh; Peterlin, B Matija

    2012-10-19

    By phosphorylating elongation factors and the C-terminal domain of RNA polymerase II, the positive transcription elongation factor b (P-TEFb) is the critical kinase for transcription elongation and co-transcriptional processing of eukaryotic genes. It exists in inactive small nuclear ribonucleoprotein (7SK snRNP) and active (free P-TEFb) complexes in cells. The P-TEFb equilibrium determines the state of cellular activation, proliferation, and differentiation. Free P-TEFb, which is required for growth, can be recruited to RNA polymerase II via transcription factors, BRD4, or the super elongation complex (SEC). UV light, various signaling cascades, transcriptional blockade, or compounds such as hexamethylene bisacetamide (HMBA), suberoylanilide hydroxamic acid (SAHA), and other histone deacetylase inhibitors lead to a rapid release of free P-TEFb, followed by its reassembly into the 7SK snRNP. As a consequence, transcription of HEXIM1, a critical 7SK snRNP subunit, and HIV is induced. In this study, we found that a bromodomain and extra-terminal (BET) bromodomain inhibitor, JQ1, which inhibits BRD4 by blocking its association with chromatin, also leads to the rapid release of free P-TEFb from the 7SK snRNP. Indeed, JQ1 transiently increased levels of free P-TEFb and BRD4·P-TEFb and SEC·P-TEFb complexes in cells. As a consequence, the levels of HEXIM1 and HIV proteins rose. Importantly, the knockdown of ELL2, a subunit of the SEC, blocked the ability of JQ1 to increase HIV transcription. Finally, the effects of JQ1 and HMBA or SAHA on the P-TEFb equilibrium were cooperative. We conclude that HMBA, SAHA, and JQ1 affect transcription elongation by a similar and convergent mechanism.

  11. THE INFLUENCE OF HYDROLOGICAL DATA QUALITY ON ESTIMATING THE RUNOFF FROM CITY CATCHMENT ON THE EXAMPLE OF STRZYŻA STEAM IN GDAŃSK

    Directory of Open Access Journals (Sweden)

    Michał Szydłowski

    2015-10-01

    Full Text Available Channel flow control and prediction of waterbodies reaction on heavy rainfalls or rapid snowmelt become critical when forecasting the flood risk in urban catchments. A very good example of both problematic and carrying a real threat to urban infrastructure is the Strzyża Creek in Gdańsk. In years 2011–2013 undertook the implementation of storm water monitoring system in the Strzyża Creek basin in Gdańsk. Since that time, it has become possible to collect valuable hydrological data on rainfall and flows in the strongly diversified urbanized districts of the city. Currently, the work is underway to extend the Strzyża Creek monitoring system. The ongoing research project is being realized again in years 2015–2017. The main aims of a project are: development of the methods of flood control, expansion of the existing rainfall-runoff and storage monitoring system in the basin and integrated water quantity and quality analysis, since the Strzyża Creek is one of the main stormwater collectors in Gdańsk. The article presents an example of using continuous data collected by the hydrological monitoring system to determine the annual runoff coefficient. The results were compared with the same parameter estimated on the basis of monthly instantaneous flow measurements and rainfall observations from the precipitation gauge localized in the vicinity of the analyzed catchment.

  12. Phenolic phytochemicals derived from red pine (Pinus densiflora) inhibit the invasion and migration of SK-Hep-1 human hepatocellular carcinoma cells.

    Science.gov (United States)

    Lee, Sang Jun; Lee, Ki Won; Hur, Haeng Jeon; Chun, Ji Young; Kim, Seo Young; Lee, Hyong Joo

    2007-01-01

    Considerable attention has recently been focused on identifying chemopreventive phytochemicals derived from medicinal plants. Here, we analyzed phenolic phytochemicals from red pine (RP) leaves and found epigallocatechin gallate (EGCG) and epigallocatechin (EGC), and catechin gallate (CG) as their major phenolic phytochemicals. This article also investigated whether RP leaf extract and its phenolic phytochemicals inhibit the invasion of SK-Hep-1 human hepatocellular carcinoma cells (SK-Hep-1 cells). RP suppressed the invasion and the migration of SK-Hep-1 cells. EGCG and CG also inhibited the invasion and migration, with EGC exhibiting a lower efficacy. Matrix metalloproteinases (MMPs), particularly gelatinase-A (MMP-2) and gelatinase-B (MMP-9), degrade components of the basement membrane and are strongly implicated in invasion and metastasis formation of malignant tumors. RP suppressed both MMP-2 and MMP-9 activities. EGCG and CG reduced the activities of MMP-9 and MMP-2 in a dose-dependent manner, with EGC exhibiting a lower efficacy on both MMPs. Our results suggest that RP inhibits tumor invasion and migration, which may be attributed to the effects of EGCG and CG. In particular, EGCG plays a key role in the efficacy of RP against hepatocarcinogenesis.

  13. Stjórnvald eða silkihúfa. Um hlutverk skólanefnda í íslenskum framhaldsskólum

    Directory of Open Access Journals (Sweden)

    Magnús Ingvarsson

    2008-12-01

    Full Text Available Þessi grein er byggð á rannsókn sem gerð var á starfsemi skólanefnda í íslenskum framhaldsskólum og var hún lokaverkefni til meistaraprófs í opinberri stjórnsýslu við Háskóla Íslands vorið 2008. Í greininni eru dregin saman helstu atriðin í rannsókninni. Þess ber þó að geta að síðan rannsóknin var gerð voru ný framhaldsskólalög samþykkt, en samkvæmt nýju lögunum breytist starfsemi skólanefnda lítillega og þau færast aðeins nær því að endurspegla raunveruleg störf nefndanna.

  14. Macrophage inhibitory cytokine-1 transactivates ErbB family receptors via the activation of Src in SK-BR-3 human breast cancer cells.

    Science.gov (United States)

    Park, Yun Jung; Lee, Hansoo; Lee, Jeong-Hyung

    2010-02-01

    The function of macrophage inhibitory cytokine-1 (MIC-1) in cancer remains controversial, and its signaling pathways remain poorly understood. In this study, we demonstrate that MIC-1 induces the transactivation of EGFR, ErbB2, and ErbB3 through the activation of c-Src in SK-BR-3 breast cells. MIC-1 induced significant phosphorylation of EGFR at Tyr845, ErbB2 at Tyr877, and ErbB3 at Tyr1289 as well as Akt and p38, Erk1/2, and JNK mitogen-activated protein kinases (MAPKs). Treatment of SK-BR-3 cells with MIC-1 increased the phosphorylation level of Src at Tyr416, and induced invasiveness of those cells. Inhibition of c-Src activity resulted in the complete abolition of MIC-1-induced phosphorylation of the EGFR, ErbB2, and ErbB3, as well as invasiveness and matrix metalloproteinase (MMP)-9 expression in SK-BR-3 cells. Collectively, these results show that MIC-1 may participate in the malignant progression of certain cancer cells through the activation of c-Src, which in turn may transactivate ErbB-family receptors.

  15. The Apoptotic Effect of Ursolic Acid on SK-Hep-1 Cells is Regulated by the PI3K/Akt, p38 and JNK MAPK Signaling Pathways.

    Science.gov (United States)

    Chuang, Wan-Ling; Lin, Ping-Yi; Lin, Hui-Chuan; Chen, Yao-Li

    2016-01-01

    Ursolic acid (UA) is a pentacyclic triterpene acid that is present in a wide variety of medicinal herbs and edible plants. This study investigated the effect of UA on apoptosis and proliferation of hepatocellular carcinoma SK-Hep-1 cells. After treatment of SK-Hep-1 cells with different concentrations of UA, we observed that cell viability was reduced in a dose- and time-dependent manner. Furthermore, there was a dose-dependent increase in the percentage of cells in the sub-G1 and G2/M phases, with cells treated with 60 μM showing the highest percentages of cells in those phases. UA-induced chromatin condensation of nuclei was observed by using DAPI staining. The western blot results revealed that exposure to UA was associated with decreased expression of the anti-apoptotic proteins Mcl-1, Bcl-xL, Bcl-2, and TCTP and increased expression of apoptosis-related proteins TNF-α, Fas, FADD, Bax, cleaved caspase-3, caspase-8, caspase-9, and PARP. Immunocytochemistry staining showed that treatment with UA resulted in increased expression of caspase-3. Moreover, exposure to UA resulted in the inhibition of the PI3K/Akt and p38 MAPK signaling pathways. These findings suggest that UA inhibits the proliferation of SK-Hep-1 cells and induces apoptosis.

  16. Choroby oraz pielęgnacja skóry głowy i włosów

    OpenAIRE

    Szklarczyk, Małgorzata; Goździalska, Anna; Jaśkiewicz, Jerzy

    2012-01-01

    Praca recenzowana / peer-reviewed paper Skóra głowy może być wrażliwa z natury lub może stać się taka w wyniku niekorzystnie działających czynników zewnętrznych, które prowadzą do osłabienia naturalnej bariery ochronnej skóry. Mieszek włosowy, z którego wyrasta włos jest bardzo wrażliwy. Zmiany w jego obrębie powodują wyraźne wahania w rozwoju włosa, w tym prowadząc do jego utraty. Problemy związane ze skórą głowy i włosów wymagają natychmiastowej reakcji, gdyż są wskaźnikiem nieprawidł...

  17. Loss of function mutation in LARP7, chaperone of 7SK ncRNA, causes a syndrome of facial dysmorphism, intellectual disability, and primordial dwarfism.

    Science.gov (United States)

    Alazami, Anas M; Al-Owain, Mohammad; Alzahrani, Fatema; Shuaib, Taghreed; Al-Shamrani, Hussain; Al-Falki, Yahya H; Al-Qahtani, Saleh M; Alsheddi, Tarfa; Colak, Dilek; Alkuraya, Fowzan S

    2012-10-01

    Primordial dwarfism (PD) is a clinically and genetically heterogeneous condition. Various molecular mechanisms are known to underlie the disease including impaired mitotic mechanics, abnormal IGF2 expression, perturbed DNA damage response, defective spliceosomal machinery, and abnormal replication licensing. Here, we describe a syndromic form of PD associated with severe intellectual disability and distinct facial features in a large multiplex Saudi family. Analysis reveals a novel underlying mechanism for PD involving depletion of 7SK, an abundant cellular noncoding RNA (ncRNA), due to mutation of its chaperone LARP7. We show that 7SK levels are tightly linked to LARP7 expression across cell lines, and that this chaperone is ubiquitously expressed in the mouse embryo. The 7SK is known to influence the expression of a wide array of genes through its inhibitory effect on the positive transcription elongation factor b (P-TEFb) as well as its competing role in HMGA1-mediated transcriptional regulation. This study documents a critical role played by ncRNA in human development and adds to the growing list of molecular mechanisms that, when perturbed, converge on the PD phenotype. © 2012 Wiley Periodicals, Inc.

  18. The effect of VMP35 supplement ingredients encapsulated in a novel Phospholipid Prodosome SK713 SLP nutrient delivery technology on properties of live human blood.

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    Kenneth Blum

    2015-09-01

    Full Text Available Background: In North America digestive malfunction in terms of disintegration, dissolution, and absorption of food and nutrients, is a widespread malady. Malabsorption is also an exacerbating factor in most chronic degenerative diseases that might benefit from dietary supplementation. The purpose of this experiment was to determine, as shown by changes in properties of live blood, whether, a novel soy-lecithin-phospholipid-nutrient encapsulation technology could promote rapid bioavailability and bioactivity of a VMP35 nutraceutical formulation encapsulated within its clustoidal multilamellar Soy Lecithin Phospholipid (SLP liquid SK713 SLP structures. Method: Changes in peripheral blood smears from 38 subjects were measured utilizing peripheral live blood cell imaging (LBCI with phase contrast microscopy. Results: Compared to baseline and control, consistently and reproducibly, the SK713 SLP technology effected positive changes in the blood as demonstrated by observable morphological, hematological and rheological changes five minutes from intake and sustained for at least 30 minutes post intake. Conclusions: These results showed that the SK713 SLP system makes an important contribution by increasing the potential benefits of dietary supplementation to those patients with compromised digestive processes. We encourage additional research on this novel delivery system believing that it has potential impact on future therapy.

  19. Torre de RTVE en el polígono 35 de la Avenida de la Paz Madrid/España

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    Miret Corral, Fernando

    1982-06-01

    Full Text Available The present number of Revista Informes, in its section «Works and projects», of a monographic character, deals with the recently finished RTVE tower in Madrid. It contemplates, in an exhaustive manner, all the aspects of the construction of a work of such singular characteristics, such as the general planning and concept, constructively and structurally, urbanistic and plastic, each of which is developed by the technicians and specialists who have intervened both in the project and in its execution. A very precise definition is given of the geometric characteristics of the tower, providing complete data referring to the foundation, shaft capitel and platforms, stairways and nucleous of elevators, mast for antennas, etc., with a concrete reference to the mechanical requirements of each element of the bases of hypothesis of calculation of the organisation and auxiliary means employed, with special emphasis on the description of the system of sliding shuttering employed, and likewise the aspects of safety and organisation of the work.El presente número de la Revista Informes ocupa su sección «Obras y proyectos», con carácter monográfico, a la recientemente terminada torre de RTVE en Madrid. En la misma se contemplan, de una manera exhaustiva, todos los aspectos que implican la construcción de una obra de características tan singulares, como son los de planteamiento general y de concepto, constructivos y estructurales en todas sus vertientes, urbanísticos y plásticos, cada uno de los cuales es desarrollado por los técnicos y especialistas que han intervenido tanto en el proyecto como en la ejecución. Se proporciona una definición muy precisa de las características geométricas de la torre, aportándose datos completos referidos a la cimentación, fuste, copa o capitel y plataformas, escaleras y núcleo de ascensores, mástil para antenas, etc., con un apunte concreto de las solicitaciones mecánicas que soporta cada elemento de las

  20. Translating E-Mental Health Into Practice: What Are the Barriers and Enablers to E-Mental Health Implementation by Aboriginal and Torres Strait Islander Health Professionals?

    Science.gov (United States)

    Singer, Judy; DuBois, Simon; Hyde, Kelly

    2017-01-01

    Background With increasing evidence for the effectiveness of e-mental health interventions for enhancing mental health and well-being, a growing challenge is how to translate promising research findings into service delivery contexts. A 2012 e-mental health initiative by the Australian Federal Government (eMHPrac) has sought to address the issue through several strategies, one of which has been to train different health professional workforces in e-mental health (e-MH). Objective The aim of the study was to report on the barriers and enablers of e-MH uptake in a cohort of predominantly Aboriginal and Torres Strait Islander health professionals (21 Indigenous, 5 non-Indigenous) who occupied mainly support or case management roles within their organizations. Methods A 3- or 2-day e-MH training program was followed by up to 5 consultation sessions (mean 2.4 sessions) provided by the 2 trainers. The trainer-consultants provided written reports on each of the 30 consultation sessions for 7 consultation groups. They were also interviewed as part of the study. The written reports and interview data were thematically analyzed by 2 members of the research team. Results Uptake of e-MH among the consultation group was moderate (22%-30% of participants). There were significant organizational barriers to uptake resulting from procedural and administrative problems, demanding workloads, prohibitive policies, and a lack of fit between the organizational culture and the introduction of new technologies. Personal barriers included participant beliefs about the applicability of e-MH to certain populations, and workers’ lack of confidence and skills. However, enthusiastic managers and tech-savvy champions could provide a counter-balance as organizational enablers of e-MH; and the consultation sessions themselves appear to have enhanced skills and confidence, shifted attitudes to new technologies, and seeded a perception that e-MH could be a valuable health education resource