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Sample records for satellite-derived normalized difference

  1. Using the satellite-derived normalized difference vegetation index (NDVI) to explain ranging patterns in a lek-breeding antelope: the importance of scale.

    Science.gov (United States)

    Bro-Jørgensen, Jakob; Brown, Molly E; Pettorelli, Nathalie

    2008-11-01

    Lek-breeding species are characterized by a negative association between territorial resource availability and male mating success; however, the impact of resources on the overall distribution patterns of the two sexes in lek systems is not clear. The normalized difference vegetation index (NDVI) has recently emerged as a powerful proxy measure for primary productivity, allowing the links between the distributions of animals and resources to be explored. Using NDVI at four spatial resolutions, we here investigate how the distribution of the two sexes in a lek-breeding population of topi antelopes relates to resource abundance before and during the rut. We found that in the dry season preceding the rut, topi density correlated positively with NDVI at the large, but not the fine, scale. This suggests that before the rut, when resources were relatively scant, topi preferred pastures where green grass was widely abundant. The pattern was less pronounced in males, suggesting that the need for territorial attendance prevents males from tracking resources as freely as females do. During the rut, which occurs in the wet season, both male and female densities correlated negatively with NDVI at the fine scale. At this time, resources were generally plentiful and the results suggest that, rather than by resource maximization, distribution during the rut was determined by benefits of aggregating on relatively resource-poor leks for mating, and possibly antipredator, purposes. At the large scale, no correlation between density and NDVI was found during the rut in either sex, which can be explained by leks covering areas too small to be reflected at this resolution. The study illustrates that when investigating spatial organization, it is important: (1) to choose the appropriate analytic scale, and (2) to consider behavioural as well as strictly ecological factors.

  2. Variations in satellite-derived carbon dioxide over different regions of China from 2003 to 2011

    Science.gov (United States)

    Xu, Yuyue; Ke, Changqing; Zhan, Wenfeng; Li, Haidong; Yao, Ling

    2017-02-01

    Variations of CO2 mole fraction (XCO2) on a global or country-wide scale have been widely examined, while the regional differences within China remain unclear because of the huge differences in the stage of economic development and climatic diversities. In this study, the variations of monthly and yearly XCO2 from 2003 to 2011 are analyzed for the entire China and its six geographical regions. Atmospheric Infrared Sounder (AIRS) data, which are mainly sensitive in the mid-troposphere, were used. During 2003-2011, the mean annual XCO2 increased from 375.7 ± 3.3 to 392.5 ± 3.5 ppm, at a rate of +2.10 ppm/year, which is similar to the previous rate during 2003-2008 (+2.09 ppm/year). However, there are two new findings for different regions of China. First, the XCO2 growth rate and its seasonal amplitude were higher in Northern China than in Southern China. The growth rates of XCO2 over North-East, North, and North-West during 2003-2011 are 2.18, 2.17, and 2.13 ppm/year, respectively, while they are 2.03, 2.05, and 2.09 ppm/year over South-East, Central, and South-West, respectively. The seasonal CO2 fluctuations over the North-East and North are larger than other regions. Second, the highest monthly mean XCO2 of mid-troposphere occurs during April to May for different regions of China in 2003-2011, while the lowest XCO2 is September for southern China and January for northern China. These results offer valuable insights into the regional differences of XCO2 within China.

  3. Varying applicability of four different satellite-derived soil moisture products to global gridded crop model evaluation

    Science.gov (United States)

    Sakai, Toru; Iizumi, Toshichika; Okada, Masashi; Nishimori, Motoki; Grünwald, Thomas; Prueger, John; Cescatti, Alessandro; Korres, Wolfgang; Schmidt, Marius; Carrara, Arnaud; Loubet, Benjamin; Ceschia, Eric

    2016-06-01

    Satellite-derived daily surface soil moisture products have been increasingly available, but their applicability to global gridded crop model (GGCM) evaluation is unclear. This study compares four different soil moisture products with the flux tower site observation at 18 cropland sites across the world where either of maize, soybean, rice and wheat is grown. These products include the first and second versions of Climate Change Initiative Soil Moisture (CCISM-1 and CCISM-2) datasets distributed by the European Space Agency and two different AMSR-E (Advanced Microwave Scanning Radiometer-Earth Observing System)-derived soil moisture datasets, separately provided by the Japan Aerospace Exploration Agency (AMSRE-J) and U.S. National Aeronautics and Space Administration (AMSRE-N). The comparison demonstrates varying reliability of these products in representing major characteristics of temporal pattern of cropland soil moisture by product and crop. Possible reasons for the varying reliability include the differences in sensors, algorithms, bands and criteria used when estimating soil moisture. Both the CCISM-1 and CCISM-2 products appear the most reliable for soybean- and wheat-growing area. However, the percentage of valid data of these products is always lower than other products due to relatively strict criteria when merging data derived from multiple sources, although the CCISM-2 product has much more data with valid retrievals than the CCISM-1 product. The reliability of the AMSRE-J product is the highest for maize- and rice-growing areas and comparable to or slightly lower than the CCISM products for soybean- and wheat-growing areas. The AMSRE-N is the least reliable in most location-crop combinations. The reliability of the products for rice-growing area is far lower than that of other upland crops likely due to the extensive use of irrigation and patch distribution of rice paddy in the area examined here. We conclude that the CCISM-1, CCISM-2 and AMSRE

  4. Migratory herbivorous waterfowl track satellite-derived green wave index.

    Science.gov (United States)

    Shariatinajafabadi, Mitra; Wang, Tiejun; Skidmore, Andrew K; Toxopeus, Albertus G; Kölzsch, Andrea; Nolet, Bart A; Exo, Klaus-Michael; Griffin, Larry; Stahl, Julia; Cabot, David

    2014-01-01

    Many migrating herbivores rely on plant biomass to fuel their life cycles and have adapted to following changes in plant quality through time. The green wave hypothesis predicts that herbivorous waterfowl will follow the wave of food availability and quality during their spring migration. However, testing this hypothesis is hampered by the large geographical range these birds cover. The satellite-derived normalized difference vegetation index (NDVI) time series is an ideal proxy indicator for the development of plant biomass and quality across a broad spatial area. A derived index, the green wave index (GWI), has been successfully used to link altitudinal and latitudinal migration of mammals to spatio-temporal variations in food quality and quantity. To date, this index has not been used to test the green wave hypothesis for individual avian herbivores. Here, we use the satellite-derived GWI to examine the green wave hypothesis with respect to GPS-tracked individual barnacle geese from three flyway populations (Russian n = 12, Svalbard n = 8, and Greenland n = 7). Data were collected over three years (2008-2010). Our results showed that the Russian and Svalbard barnacle geese followed the middle stage of the green wave (GWI 40-60%), while the Greenland geese followed an earlier stage (GWI 20-40%). Despite these differences among geese populations, the phase of vegetation greenness encountered by the GPS-tracked geese was close to the 50% GWI (i.e. the assumed date of peak nitrogen concentration), thereby implying that barnacle geese track high quality food during their spring migration. To our knowledge, this is the first time that the migration of individual avian herbivores has been successfully studied with respect to vegetation phenology using the satellite-derived GWI. Our results offer further support for the green wave hypothesis applying to long-distance migrants on a larger scale.

  5. Migratory herbivorous waterfowl track satellite-derived green wave index.

    Directory of Open Access Journals (Sweden)

    Mitra Shariatinajafabadi

    Full Text Available Many migrating herbivores rely on plant biomass to fuel their life cycles and have adapted to following changes in plant quality through time. The green wave hypothesis predicts that herbivorous waterfowl will follow the wave of food availability and quality during their spring migration. However, testing this hypothesis is hampered by the large geographical range these birds cover. The satellite-derived normalized difference vegetation index (NDVI time series is an ideal proxy indicator for the development of plant biomass and quality across a broad spatial area. A derived index, the green wave index (GWI, has been successfully used to link altitudinal and latitudinal migration of mammals to spatio-temporal variations in food quality and quantity. To date, this index has not been used to test the green wave hypothesis for individual avian herbivores. Here, we use the satellite-derived GWI to examine the green wave hypothesis with respect to GPS-tracked individual barnacle geese from three flyway populations (Russian n = 12, Svalbard n = 8, and Greenland n = 7. Data were collected over three years (2008-2010. Our results showed that the Russian and Svalbard barnacle geese followed the middle stage of the green wave (GWI 40-60%, while the Greenland geese followed an earlier stage (GWI 20-40%. Despite these differences among geese populations, the phase of vegetation greenness encountered by the GPS-tracked geese was close to the 50% GWI (i.e. the assumed date of peak nitrogen concentration, thereby implying that barnacle geese track high quality food during their spring migration. To our knowledge, this is the first time that the migration of individual avian herbivores has been successfully studied with respect to vegetation phenology using the satellite-derived GWI. Our results offer further support for the green wave hypothesis applying to long-distance migrants on a larger scale.

  6. Estimating carbon flux phenology with satellite-derived land surface phenology and climate drivers for different biomes: a synthesis of AmeriFlux observations.

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    Wenquan Zhu

    Full Text Available Carbon Flux Phenology (CFP can affect the interannual variation in Net Ecosystem Exchange (NEE of carbon between terrestrial ecosystems and the atmosphere. In this study, we proposed a methodology to estimate CFP metrics with satellite-derived Land Surface Phenology (LSP metrics and climate drivers for 4 biomes (i.e., deciduous broadleaf forest, evergreen needleleaf forest, grasslands and croplands, using 159 site-years of NEE and climate data from 32 AmeriFlux sites and MODIS vegetation index time-series data. LSP metrics combined with optimal climate drivers can explain the variability in Start of Carbon Uptake (SCU by more than 70% and End of Carbon Uptake (ECU by more than 60%. The Root Mean Square Error (RMSE of the estimations was within 8.5 days for both SCU and ECU. The estimation performance for this methodology was primarily dependent on the optimal combination of the LSP retrieval methods, the explanatory climate drivers, the biome types, and the specific CFP metric. This methodology has a potential for allowing extrapolation of CFP metrics for biomes with a distinct and detectable seasonal cycle over large areas, based on synoptic multi-temporal optical satellite data and climate data.

  7. Estimating carbon flux phenology with satellite-derived land surface phenology and climate drivers for different biomes: a synthesis of AmeriFlux observations.

    Science.gov (United States)

    Zhu, Wenquan; Chen, Guangsheng; Jiang, Nan; Liu, Jianhong; Mou, Minjie

    2013-01-01

    Carbon Flux Phenology (CFP) can affect the interannual variation in Net Ecosystem Exchange (NEE) of carbon between terrestrial ecosystems and the atmosphere. In this study, we proposed a methodology to estimate CFP metrics with satellite-derived Land Surface Phenology (LSP) metrics and climate drivers for 4 biomes (i.e., deciduous broadleaf forest, evergreen needleleaf forest, grasslands and croplands), using 159 site-years of NEE and climate data from 32 AmeriFlux sites and MODIS vegetation index time-series data. LSP metrics combined with optimal climate drivers can explain the variability in Start of Carbon Uptake (SCU) by more than 70% and End of Carbon Uptake (ECU) by more than 60%. The Root Mean Square Error (RMSE) of the estimations was within 8.5 days for both SCU and ECU. The estimation performance for this methodology was primarily dependent on the optimal combination of the LSP retrieval methods, the explanatory climate drivers, the biome types, and the specific CFP metric. This methodology has a potential for allowing extrapolation of CFP metrics for biomes with a distinct and detectable seasonal cycle over large areas, based on synoptic multi-temporal optical satellite data and climate data.

  8. Estimating Ground-Level Particulate Matter (PM) Concentration using Satellite-derived Aerosol Optical Depth (AOD)

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    Park, Seohui; Im, Jungho

    2017-04-01

    Atmospheric aerosols are strongly associated with adverse human health effects. In particular, particulate matter less than 10 micrometers and 2.5 micrometers (i.e., PM10 and PM2.5, respectively) can cause cardiovascular and lung diseases such as asthma and chronic obstructive pulmonary disease (COPD). Air quality including PM has typically been monitored using station-based in-situ measurements over the world. However, in situ measurements do not provide spatial continuity over large areas. An alternative approach is to use satellite remote sensing as it provides data over vast areas at high temporal resolution. The literature shows that PM concentrations are related with Aerosol Optical Depth (AOD) that is derived from satellite observations, but it is still difficult to identify PM concentrations directly from AOD. Some studies used statistical approaches for estimating PM concentrations from AOD while some others combined numerical models and satellite-derived AOD. In this study, satellite-derived products were used to estimate ground PM concentrations based on machine learning over South Korea. Satellite-derived products include AOD from Geostationary Ocean Color Imager (GOCI), precipitation from Tropical Rainfall Measuring Mission (TRMM), soil moisture from AMSR-2, elevation from Shuttle Radar Topography Mission (SRTM), and land cover, land surface temperature and normalized difference vegetation index (NDVI) from Moderate Resolution Imaging Spectroradiometer (MODIS). PM concentrations data were collected from 318 stations. A statistical ordinary least squares (OLS) approach was also tested and compared with the machine learning approach (i.e., random forest). PM concentration was estimated during spring season (from March to May) in 2015 that typically shows high concentration of PM. The randomly selected 80% of data were used for model calibration and the remaining 20% were used for validation. The developed models were further tested for prediction of PM

  9. Poverty, health and satellite-derived vegetation indices: their inter-spatial relationship in West Africa.

    Science.gov (United States)

    Sedda, Luigi; Tatem, Andrew J; Morley, David W; Atkinson, Peter M; Wardrop, Nicola A; Pezzulo, Carla; Sorichetta, Alessandro; Kuleszo, Joanna; Rogers, David J

    2015-03-01

    Previous analyses have shown the individual correlations between poverty, health and satellite-derived vegetation indices such as the normalized difference vegetation index (NDVI). However, generally these analyses did not explore the statistical interconnections between poverty, health outcomes and NDVI. In this research aspatial methods (principal component analysis) and spatial models (variography, factorial kriging and cokriging) were applied to investigate the correlations and spatial relationships between intensity of poverty, health (expressed as child mortality and undernutrition), and NDVI for a large area of West Africa. This research showed that the intensity of poverty (and hence child mortality and nutrition) varies inversely with NDVI. From the spatial point-of-view, similarities in the spatial variation of intensity of poverty and NDVI were found. These results highlight the utility of satellite-based metrics for poverty models including health and ecological components and, in general for large scale analysis, estimation and optimisation of multidimensional poverty metrics. However, it also stresses the need for further studies on the causes of the association between NDVI, health and poverty. Once these relationships are confirmed and better understood, the presence of this ecological component in poverty metrics has the potential to facilitate the analysis of the impacts of climate change on the rural populations afflicted by poverty and child mortality. © The Author 2015. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. "Ser diferente é normal?"/"Being different: is it normal?"

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    Viviane Veras

    2007-01-01

    Full Text Available A pergunta título deste trabalho retoma o slogan “Ser diferente é normal”, que é parte da campanha criada para uma organização não-governamental que atende portadores de Síndrome de Down. O objetivo é a inclusão social da pessoa com deficiência e o primeiro passo foi propor a inclusão de um grupo de diferentes no grupo dito normal. No vídeo de lançamento da campanha, o diferente, identificado como normal, é mostrado por meio de exemplos – um negro com cabelo black-power, um skin-head, um corpo tatuado, um corpo feminino halterofílico, uma família hippie, uma garota com síndrome de Down. A visão da adolescente dançando reduz, de certo modo, o efeito imaginário que vai além da síndrome, uma vez que apenas o corpo com seus olhinhos puxados se destacam, e não se interrogam questões cognitivas. Minha proposta é refletir sobre o estatuto paradoxal do exemplo, tal como é trabalhado nesse vídeo: se, por definição, um exemplo mostra de fato seu pertencimento a uma classe, pode-se concluir que é exatamente por ser exemplar que ele se encontra fora dela, no exato momento em que a exibe e define. The question in the title of this paper refers to the slogan "ser diferente é normal" ("It´s normal to be different", which is part of a campaign created for a NGO that supports people with Down syndrome. The objective of the campaign is to promote the social inclusion of individuals with Down syndrome, and the first step was to propose the inclusion of a group of "differents" in the so-called normal group. The film launching the campaign shows the different identified as normal by means of examples: a black man exhibiting blackpower haircut, a skin-head, a tattooed body, an over-athletic female body, a hippie family and a girl with Down syndrome. The vision of the dancing teenager lessens the imaginary effect that surpasses the syndrome, since only her body and her little oriental eyes stand out and no cognitive issues are

  11. How Different are Normal and Barred Spirals?

    CERN Document Server

    Bergh, Sidney van den

    2011-01-01

    No significant color differences are found between normal and barred spirals over the range of Hubble stages a - ab - b - bc. Furthermore, no significant difference is seen between the luminosity distributions of normal and barred galaxies over the same range of Hubble stages. However, SBc galaxies are found to be systematically fainter than Sc galaxies at 99% confidence. The observation that normal and barred spirals with Hubble stages a - ab - b - bc have indistinguishable intrinsic colors hints at the possibility that the bars in such spiral galaxies might be ephemeral structures. Finally, it is pointed out that lenticular galaxies of types S0 and SB0 are systematically fainter than are other early-type galaxies, suggesting that such galaxies are situated on evolutionary tracks that differ systematically from those of galaxies that lie along the E - Sa - Sb -Sc and E - SBa - SBb - SBc sequences.

  12. Statistical modeling of phenological phases in Poland based on coupling satellite derived products and gridded meteorological data

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    Czernecki, Bartosz; Jabłońska, Katarzyna; Nowosad, Jakub

    2016-04-01

    The aim of the study was to create and evaluate different statistical models for reconstructing and predicting selected phenological phases. This issue is of particular importance in Poland where national-wide phenological monitoring was abandoned in the middle of 1990s and the reactivated network was established in 2006. Authors decided to evaluate possibilities of using a wide-range of statistical modeling techniques to create synthetic archive dataset. Additionally, a robust tool for predicting the most distinguishable phenophases using only free of charge data as predictors was created. Study period covers the years 2007-2014 and contains only quality-controlled dataset of 10 species and 14 phenophases. Phenological data used in this study originates from the manual observations network run by the Institute of Meteorology and Water Management - National Research Institute (IMGW-PIB). Three kind of data sources were used as predictors: (i) satellite derived products, (ii) preprocessed gridded meteorological data, and (iii) spatial properties (longitude, latitude, altitude) of the monitoring site. Moderate-Resolution Imaging Spectroradiometer (MODIS) level-3 vegetation products were used for detecting onset dates of particular phenophases. Following indices were used: Normalized Difference Vegetation Index (NDVI), Enhanced Vegetation Index (EVI), Leaf Area Index (LAI), and Fraction of Photosynthetically Active Radiation (fPAR). Additionally, Interactive Multisensor Snow and Ice Mapping System (IMS) products were chosen to detect occurrence of snow cover. Due to highly noisy data, authors decided to take into account pixel reliability information. Besides satellite derived products (NDVI, EVI, FPAR, LAI, Snow cover), a wide group of observational data and agrometeorological indices derived from the European Climate Assessment & Dataset (ECA&D) were used as a potential predictors: cumulative growing degree days (GDD), cumulative growing precipitation days (GPD

  13. Investigation of Vegetation Dynamics using Long-Term Normalized Difference Vegetation Index Time-Series

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    Tamara Bellone

    2009-01-01

    Full Text Available Problem statement: The Normalized Difference Vegetation Index (NDVI is the most extensively used satellite-derived index of vegetation health and density. Since climate is one of the most important factors affecting vegetation condition, satellite-derived vegetation indexes have been often used to evaluate climatic and environmental changes at regional and global scale. The proposed study attempted to investigate the temporal vegetation dynamics in the whole Africa using historical NDVI time-series. Approach: For this aim, 15 day maximum value NDVI composites at 8 km spatial resolution produced from the NASA Global Inventory Mapping and Monitoring System (GIMMS had been used. They were derived from data collected daily by NOAA AVHRR satellites. The AVHRR NDVI GIMMS dataset was freely available and gives global coverage over an extensive time period. First of all, the selected NDVI base data had been geometrically pre-processed and organized into a historical database implemented in order to grant their spatial integration. Starting from this archive, monthly and yearly NDVI historical time-series, extended from 1982-2006, had been then developed and analysed on a pixel basis. Several routines hade been developed in IDL (Interactive Data Language programming tool with the purpose of applying suitable statistical analysis techniques to the historical information in the database in order to identify the long-term trend components of generated NDVI time-series and extract vegetation dynamics. Specific tests had been then considered in order to define the validity of results. Results: The existence of clear regional trends of NDVI, both decreasing and increasing had been showed, which helped to highlight areas subject, respectively to reduction or increase in vegetation greenness. Conclusion: As the relationship between the NDVI and vegetation productivity was well established, these estimated long-term trend components may be also, with much more

  14. Portable Instrument for Normalized Difference Vegetation Index

    Institute of Scientific and Technical Information of China (English)

    ZHOU Han-chang; ZHAO Chun-jiang; XUE Xu-zhang; HAO Xiao-jian

    2004-01-01

    By using four specially designed narrow bandpass filters and photodetectors in the instrument, the incident and reflected radiances of sun light on the vegetation are optically sensed, at the red and near infrared bands, then the normalized difference vegetation index(NDVI) is processed by a microprocessor. Compared with conventional spectrometer measuring method of NDVI, the instrument is easy to be used, compact, light and low-cost.

  15. How dementia differs from normal ageing.

    Science.gov (United States)

    Ginesi, Laura; Jenkins, Catharine; Keenan, Bernie

    Dementia is a collective name for a set of symptoms that include memory loss, mood changes, confusion and increasing difficulty with everyday tasks. This four-part series provides an overview of dementia and its treatment, from its causes and pathophysiology to diagnosis and the nurse's role in its management. This first article reviews the main forms of dementia and how research is shedding new light on the differences between dementia and normal ageing.

  16. Online Assessment of Satellite-Derived Global Precipitation Products

    Science.gov (United States)

    Liu, Zhong; Ostrenga, D.; Teng, W.; Kempler, S.

    2012-01-01

    Precipitation is difficult to measure and predict. Each year droughts and floods cause severe property damages and human casualties around the world. Accurate measurement and forecast are important for mitigation and preparedness efforts. Significant progress has been made over the past decade in satellite precipitation product development. In particular, products' spatial and temporal resolutions as well as timely availability have been improved by blended techniques. Their resulting products are widely used in various research and applications. However biases and uncertainties are common among precipitation products and an obstacle exists in quickly gaining knowledge of product quality, biases and behavior at a local or regional scale, namely user defined areas or points of interest. Current online inter-comparison and validation services have not addressed this issue adequately. To address this issue, we have developed a prototype to inter-compare satellite derived daily products in the TRMM Online Visualization and Analysis System (TOVAS). Despite its limited functionality and datasets, users can use this tool to generate customized plots within the United States for 2005. In addition, users can download customized data for further analysis, e.g. comparing their gauge data. To meet increasing demands, we plan to increase the temporal coverage and expanded the spatial coverage from the United States to the globe. More products have been added as well. In this poster, we present two new tools: Inter-comparison of 3B42RT and 3B42 Inter-comparison of V6 and V7 TRMM L-3 monthly products The future plans include integrating IPWG (International Precipitation Working Group) Validation Algorithms/statistics, allowing users to generate customized plots and data. In addition, we will expand the current daily products to monthly and their climatology products. Whenever the TRMM science team changes their product version number, users would like to know the differences by

  17. Trends in a satellite-derived vegetation index and environmental variables in a restored brackish lagoon

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    Ji Yoon Kim

    2015-07-01

    Full Text Available We evaluated relative influence of climatic variables on the plant productivity after lagoon restoration. Chilika Lagoon, the largest brackish lake ecosystem in East Asia, experienced severe problems such as excessive dominance of freshwater exotic plants and rapid debasement of biodiversity associated with decreased hydrologic connectivity between the lagoon and the ocean. To halt the degradation of the lagoon ecosystem, the Chilika Development Authority implemented a restoration project, creating a new channel to penetrate the barrier beach of the lagoon. Using a satellite-derived normalized difference vegetation index (NDVI dataset, we compared the trend of vegetation changes after the lagoon restoration, from April 1998 to May 2014. The time series of NDVI data were decomposed into trend, seasonal, and random components using a local regression method. The results were visualized to understand the traits of spatial distribution in the lagoon. The NDVI trend, indicative of primary productivity, decreased rapidly during the restoration period, and gradually increased (slope coefficient: 2.1×10−4, p<0.05 after two years of restoration. Level of seawater exchange had more influences on plant productivity than local precipitation in the restored lagoon. Higher El Niño/Southern Oscillation increased sea level pressure, and caused intrusion of seawater into the lagoon, and the subsequently elevated salinity decreased the annual mean NDVI. Our findings suggest that lagoon restoration plans for enhancing interconnectivity with the ocean should consider oceanographic effects due to meteorological forcing, and long-term NDVI results can be used as a valuable index for adaptive management of the restoration site.

  18. Satellite-derived methane emissions from inundation in Bangladesh

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    Peters, C. N.; Bennartz, R.; Hornberger, G. M.

    2017-05-01

    The uncertainty in methane (CH4) source strength of rice fields and wetlands is particularly high in South Asia CH4 budgets. We used satellite observations of CH4 column mixing ratios from Atmospheric Infrared Sounder (AIRS), Scanning Imaging Absorption Spectrometer for Atmospheric Chartography (SCIAMACHY), and Greenhouse Gases Observing Satellite (GOSAT) to estimate the contribution of Bangladesh emissions to atmospheric CH4 concentrations. Using satellite-derived inundation area as a proxy for source area, we developed a simple inverse advection model that estimates average annual CH4 surface fluxes to be 4, 9, and 19 mg CH4 m-2 h-1 in AIRS, SCIAMACHY, and GOSAT, respectively. Despite this variability, our flux estimates varied over a significantly narrower range than reported values for CH4 surface fluxes from a survey of 32 studies reporting ground-based observations between 0 and 260 mg CH4 m-2 h-1. Upscaling our satellite-derived surface flux estimates, we estimated total annual CH4 emissions for Bangladesh to be 1.3 ± 3.2, 1.8 ± 2.0, 3.1 ± 1.6 Tg yr-1, depending on the satellite. Our estimates of total emissions are in line with the median of total emission values for Bangladesh reported in earlier studies.

  19. Preliminary survey on site-adaptation techniques for satellite-derived and reanalysis solar radiation datasets

    Energy Technology Data Exchange (ETDEWEB)

    Polo, J.; Wilbert, S.; Ruiz-Arias, J. A.; Meyer, R.; Gueymard, C.; Súri, M.; Martín, L.; Mieslinger, T.; Blanc, P.; Grant, I.; Boland, J.; Ineichen, P.; Remund, J.; Escobar, R.; Troccoli, A.; Sengupta, M.; Nielsen, K. P.; Renne, D.; Geuder, N.; Cebecauer, T.

    2016-07-01

    At any site, the bankability of a projected solar power plant largely depends on the accuracy and general quality of the solar radiation data generated during the solar resource assessment phase. The term 'site adaptation' has recently started to be used in the framework of solar energy projects to refer to the improvement that can be achieved in satellite-derived solar irradiance and model data when short-term local ground measurements are used to correct systematic errors and bias in the original dataset. This contribution presents a preliminary survey of different possible techniques that can improve long-term satellite-derived and model-derived solar radiation data through the use of short-term on-site ground measurements. The possible approaches that are reported here may be applied in different ways, depending on the origin and characteristics of the uncertainties in the modeled data. This work, which is the first step of a forthcoming in-depth assessment of methodologies for site adaptation, has been done within the framework of the International Energy Agency Solar Heating and Cooling Programme Task 46 'Solar Resource Assessment and Forecasting.'

  20. A Combined Satellite-Derived Drought Indicator to Support Humanitarian Aid Organizations

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    Markus Enenkel

    2016-04-01

    Full Text Available Governments, aid organizations and researchers are struggling with the complexity of detecting and monitoring drought events, which leads to weaknesses regarding the translation of early warnings into action. Embedded in an advanced decision-support framework for Doctors without Borders (Médecins sans Frontières, this study focuses on identifying the added-value of combining different satellite-derived datasets for drought monitoring and forecasting in Ethiopia. The core of the study is the improvement of an existing drought index via methodical adaptations and the integration of various satellite-derived datasets. The resulting Enhanced Combined Drought Index (ECDI links four input datasets (rainfall, soil moisture, land surface temperature and vegetation status. The respective weight of each input dataset is calculated for every grid point at a spatial resolution of 0.25 degrees (roughly 28 kilometers. In the case of data gaps in one input dataset, the weights are automatically redistributed to other available variables. Ranking the years 1992 to 2014 according to the ECDI-based warning levels allows for the identification of all large-scale drought events in Ethiopia. Our results also indicate a good match between the ECDI-based drought warning levels and reported drought impacts for both the start and the end of the season.

  1. Linking Satellite-Derived Fire Counts to Satellite-Derived Weather Data in Fire Prediction Models to Forecast Extreme Fires in Siberia

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    Westberg, D. J.; Soja, A. J.; Stackhouse, P. W.

    2009-12-01

    Fire is the dominant disturbance that precipitates ecosystem change in boreal regions, and fire is largely under the control of weather and climate. Fire frequency, fire severity, area burned and fire season length are predicted to increase in boreal regions under climate change scenarios. Therefore to predict fire weather and ecosystem change, we must understand the factors that influence fire regimes and at what scale these are viable. The Canadian Fire Weather Index (FWI), developed by the Canadian Forestry Service, is used for this comparison, and it is calculated using local noon surface-level air temperature, relative humidity, wind speed, and daily (noon-noon) rainfall. The FWI assesses daily forest fire burning potential. Large-scale FWI are calculated at the NASA Langley Research Center (LaRC) using NASA Goddard Earth Observing System version 4 (GEOS-4) large-scale reanalysis and NASA Global Precipitation Climatology Project (GPCP) data. The GEOS-4 reanalysis weather data are 3-hourly interpolated to 1-hourly data at a 1ox1o resolution and the GPCP precipitation data are also at 1ox1o resolution. In previous work focusing on the fire season in Siberia in 1999 and 2002, we have shown the combination of GEOS-4 weather data and Global Precipitation Climatology Project (GPCP) precipitation data compares well to ground-based weather data when used as inputs for FWI calculation. The density and accuracy of Siberian surface station data can be limited, which leads to results that are not representative of the spatial reality. GEOS-4/GPCP-dervied FWI can serve to spatially enhance current and historic FWI, because these data are spatially and temporally consistency. The surface station and model reanalysis derived fire weather indices compared well spatially, temporally and quantitatively, and increased fire activity compares well with increasing FWI ratings. To continue our previous work, we statistically compare satellite-derived fire counts to FWI categories at

  2. Comparison of Satellite-Derived and In-Situ Observations of Ice and Snow Surface Temperatures over Greenland

    Science.gov (United States)

    Hall, Dorothy K.; Box, Jason E.; Casey, Kimberly A.; Hook, Simon J.; Shuman, Christopher A.; Steffen, Konrad

    2008-01-01

    The most practical way to get a spatially broad and continuous measurements of the surface temperature in the data-sparse cryosphere is by satellite remote sensing. The uncertainties in satellite-derived LSTs must be understood to develop internally-consistent decade-scale land-surface temperature (LST) records needed for climate studies. In this work we assess satellite-derived "clear-sky" LST products from the Moderate Resolution Imaging Spectroradiometer (MODIS) and the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), and LSTs derived from the Enhanced Thematic Mapper Plus (ETM+) over snow and ice on Greenland. When possible, we compare satellite-derived LSTs with in-situ air-temperature observations from Greenland Climate Network (GC-Net) automatic-weather stations (AWS). We find that MODIS, ASTER and ETM+ provide reliable and consistent LSTs under clear-sky conditions and relatively-flat terrain over snow and ice targets over a range of temperatures from -40 to 0 C. The satellite-derived LSTs agree within a relative RMS uncertainty of approx.0.5 C. The good agreement among the LSTs derived from the various satellite instruments is especially notable since different spectral channels and different retrieval algorithms are used to calculate LST from the raw satellite data. The AWS record in-situ data at a "point" while the satellite instruments record data over an area varying in size from: 57 X 57 m (ETM+), 90 X 90 m (ASTER), or to 1 X 1 km (MODIS). Surface topography and other factors contribute to variability of LST within a pixel, thus the AWS measurements may not be representative of the LST of the pixel. Without more information on the local spatial patterns of LST, the AWS LST cannot be considered valid ground truth for the satellite measurements, with RMS uncertainty approx.2 C. Despite the relatively large AWS-derived uncertainty, we find LST data are characterized by high accuracy but have uncertain absolute precision.

  3. Initialization with diabatic heating from satellite-derived rainfall

    Science.gov (United States)

    Ma, Leiming; Chan, Johnny; Davidson, Noel E.; Turk, Joe

    2007-07-01

    In this paper, a new technique is proposed to improve initialization of a tropical cyclone (TC) prediction model using diabatic heating profiles estimated from a combination of both infrared satellite cloud imagery and satellite-derived rainfall. The method is termed Rainfall-defined Diabatic Heating, RDH. To examine the RDH performance, initialization and forecast experiments are made with the Australia Bureau of Meteorology Research Centre (BMRC) Tropical Cyclone — Limited Area Prediction System (TC-LAPS) for the case of TC Chris, which made landfall on the west coast of Australia during 3-6 Feb 2002. RDH is performed in three steps: 1) based on previous observational and numerical studies, reference diabatic heating profiles are firstly classified into three kinds: convective, stratiform or composite types; 2) NRL (Naval Research Laboratory) 3-hourly gridded satellite rainfall estimates are categorized as one of the three types according to the rain rate; 3) within a nudging phase of 24 h, the model-generated heating at each grid point during the integration is replaced by the reference heating profiles on the basis of the satellite-observed cloud top temperature and rainfall type. The results of sensitivity experiments show that RDH has a positive impact on the model initialization of TC Chris. The heating profiles generated by the model within the observed rainfall area show agreement with that of reference heating. That is, maximum heating is located in the lower troposphere for convective rainfall, and in the upper troposphere for stratiform rainfall. In response to the replaced heating and its impact on the TC structure, the model initial condition and forecasts of the track and intensity are improved.

  4. Satellite-derived sea surface height and sea surface wind data fusion for spilled oil tracking

    Science.gov (United States)

    Kozai, Katsutoshi

    2003-12-01

    An attempt is made to estimate the trajectory of the spilled oil from the sunken tanker Nakhodka occurred on January 2, 1997 in the Japan Sea by fusing two microwave sensor data, namely ERS-2 altimeter and ADEOS/NSCAT scatterometer data. In this study 'fusion' is defined as the method of more reliable prediction for the trajectory of spilled oil than before. Geostrophic current vectors are derived from ERS-2 altimeter and wind-induced drift vectors are derived from ADEOS/NSCAT scatterometer data These two different satellite-derived vectors are 'fused' together in the surface current model to estimate and evaluate the trajectory of spilled oil from the sunken tanker Nakhodka. The distribution of component of spill vector is mostly accounted for by the distribution of geostrophic velocity component during the study period with some discrepancies during March, 1997.

  5. Evaluating a satellite-based seasonal evapotranspiration product and identifying its relationship with other satellite-derived products and crop yield: A case study for Ethiopia

    Science.gov (United States)

    Tadesse, Tsegaye; Senay, Gabriel B.; Berhan, Getachew; Regassa, Teshome; Beyene, Shimelis

    2015-01-01

    Satellite-derived evapotranspiration anomalies and normalized difference vegetation index (NDVI) products from Moderate Resolution Imaging Spectroradiometer (MODIS) data are currently used for African agricultural drought monitoring and food security status assessment. In this study, a process to evaluate satellite-derived evapotranspiration (ETa) products with a geospatial statistical exploratory technique that uses NDVI, satellite-derived rainfall estimate (RFE), and crop yield data has been developed. The main goal of this study was to evaluate the ETa using the NDVI and RFE, and identify a relationship between the ETa and Ethiopia’s cereal crop (i.e., teff, sorghum, corn/maize, barley, and wheat) yields during the main rainy season. Since crop production is one of the main factors affecting food security, the evaluation of remote sensing-based seasonal ETa was done to identify the appropriateness of this tool as a proxy for monitoring vegetation condition in drought vulnerable and food insecure areas to support decision makers. The results of this study showed that the comparison between seasonal ETa and RFE produced strong correlation (R2 > 0.99) for all 41 crop growing zones in Ethiopia. The results of the spatial regression analyses of seasonal ETa and NDVI using Ordinary Least Squares and Geographically Weighted Regression showed relatively weak yearly spatial relationships (R2 cropping zones. However, for each individual crop zones, the correlation between NDVI and ETa ranged between 0.3 and 0.84 for about 44% of the cropping zones. Similarly, for each individual crop zones, the correlation (R2) between the seasonal ETa anomaly and de-trended cereal crop yield was between 0.4 and 0.82 for 76% (31 out of 41) of the crop growing zones. The preliminary results indicated that the ETa products have a good predictive potential for these 31 identified zones in Ethiopia. Decision makers may potentially use ETa products for monitoring cereal crop yields and

  6. Temporal Trends in Satellite-Derived Erythemal UVB and Implications for Ambient Sun Exposure Assessment

    Directory of Open Access Journals (Sweden)

    Marvin Langston

    2017-02-01

    Full Text Available Ultraviolet radiation (UVR has been associated with various health outcomes, including skin cancers, vitamin D insufficiency, and multiple sclerosis. Measurement of UVR has been difficult, traditionally relying on subject recall. We investigated trends in satellite-derived UVB from 1978 to 2014 within the continental United States (US to inform UVR exposure assessment and determine the potential magnitude of misclassification bias created by ignoring these trends. Monthly UVB data remotely sensed from various NASA satellites were used to investigate changes over time in the United States using linear regression with a harmonic function. Linear regression models for local geographic areas were used to make inferences across the entire study area using a global field significance test. Temporal trends were investigated across all years and separately for each satellite type due to documented differences in UVB estimation. UVB increased from 1978 to 2014 in 48% of local tests. The largest UVB increase was found in Western Nevada (0.145 kJ/m2 per five-year increment, a total 30-year increase of 0.87 kJ/m2. This largest change only represented 17% of total ambient exposure for an average January and 2% of an average July in Western Nevada. The observed trends represent cumulative UVB changes of less than a month, which are not relevant when attempting to estimate human exposure. The observation of small trends should be interpreted with caution due to measurement of satellite parameter inputs (ozone and climatological factors that may impact derived satellite UVR nearly 20% compared to ground level sources. If the observed trends hold, satellite-derived UVB data may reasonably estimate ambient UVB exposures even for outcomes with long latency phases that predate the satellite record.

  7. Satellite Derived Volcanic Ash Product Inter-Comparison in Support to SCOPE-Nowcasting

    Science.gov (United States)

    Siddans, Richard; Thomas, Gareth; Pavolonis, Mike; Bojinski, Stephan

    2016-04-01

    In support of aeronautical meteorological services, WMO organized a satellite-based volcanic ash retrieval algorithm inter-comparison activity, to improve the consistency of quantitative volcanic ash products from satellites, under the Sustained, Coordinated Processing of Environmental Satellite Data for Nowcasting (SCOPEe Nowcasting) initiative (http:/ jwww.wmo.int/pagesjprogjsatjscopee nowcasting_en.php). The aims of the intercomparison were as follows: 1. Select cases (Sarychev Peak 2009, Eyjafyallajökull 2010, Grimsvötn 2011, Puyehue-Cordón Caulle 2011, Kirishimayama 2011, Kelut 2014), and quantify the differences between satellite-derived volcanic ash cloud properties derived from different techniques and sensors; 2. Establish a basic validation protocol for satellite-derived volcanic ash cloud properties; 3. Document the strengths and weaknesses of different remote sensing approaches as a function of satellite sensor; 4. Standardize the units and quality flags associated with volcanic cloud geophysical parameters; 5. Provide recommendations to Volcanic Ash Advisory Centers (VAACs) and other users on how to best to utilize quantitative satellite products in operations; 6. Create a "road map" for future volcanic ash related scientific developments and inter-comparison/validation activities that can also be applied to SO2 clouds and emergent volcanic clouds. Volcanic ash satellite remote sensing experts from operational and research organizations were encouraged to participate in the inter-comparison activity, to establish the plans for the inter-comparison and to submit data sets. RAL was contracted by EUMETSAT to perform a systematic inter-comparison of all submitted datasets and results were reported at the WMO International Volcanic Ash Inter-comparison Meeting to held on 29 June - 2 July 2015 in Madison, WI, USA (http:/ /cimss.ssec.wisc.edujmeetings/vol_ash14). 26 different data sets were submitted, from a range of passive imagers and spectrometers and

  8. Spatial disaggregation of satellite-derived irradiance using a high-resolution digital elevation model

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz-Arias, Jose A.; Tovar-Pescador, Joaquin [Department of Physics, University of Jaen (Spain); Cebecauer, Tomas [European Commission, Joint Research Centre, Ispra (Italy); GeoModel s.r.o., Bratislava (Slovakia); Institute of Geography, Slovak Academy of Sciences, Bratislava (Slovakia); Suri, Marcel [European Commission, Joint Research Centre, Ispra (Italy); GeoModel s.r.o., Bratislava (Slovakia)

    2010-09-15

    Downscaling of the Meteosat-derived solar radiation ({proportional_to}5 km grid resolution) is based on decomposing the global irradiance and correcting the systematic bias of its components using the elevation and horizon shadowing that are derived from the SRTM-3 digital elevation model (3 arc sec resolution). The procedure first applies the elevation correction based on the difference between coarse and high spatial resolution. Global irradiance is split into direct, diffuse circumsolar and diffuse isotropic components using statistical models, and then corrections due to terrain shading and sky-view fraction are applied. The effect of reflected irradiance is analysed only in the theoretical section. The method was applied in the eastern Andalusia, Spain, and the validation was carried out for 22 days on April, July and December 2006 comparing 15-min estimates of the satellite-derived solar irradiance and observations from nine ground stations. Overall, the corrections of the satellite estimates in the studied region strongly reduced the mean bias of the estimates for clear and cloudy days from roughly 2.3% to 0.4%. (author)

  9. Spatial and Quantitative Comparison of Satellite-Derived Land Cover Products over China

    Institute of Scientific and Technical Information of China (English)

    GAO Hao; JIA Gen-Suo

    2012-01-01

    Because land cover plays an important role in global climate change studies, assessing the agreement among different land cover products is critical. Significant discrepancies have been reported among satellite-derived land cover products, especially at the regional scale. Dif- ferent classification schemes are a key obstacle to the comparison of products and are considered the main fac- tor behind the disagreement among the different products. Using a feature-based overlap metric, we investigated the degree of spatial agreement and quantified the overall and class-specific agreement among the Moderate Resolution Imaging Spectoradiometer (MODIS), Global Land Cover 2000 (GLC2000), and the National Land Cover/Use Data- sets (NLCD) products, and the author assessed the prod- ucts by ground reference data at the regional scale over China. The areas with a low degree of agreement mostly occurred in heterogeneous terrain and transition zones, while the areas with a high degree of agreement occurred in major plains and areas with homogeneous vegetation. The overall agreement of the MODIS and GLC2000 products was 50.8% and 52.9%, and the overall accuracy was 50.3% and 41.9%, respectively. Class-specific agree- ment or accuracy varied significantly. The high-agreement classes are water, grassland, cropland, snow and ice, and bare areas, whereas classes with low agreement are shru- bland and wetland in both MODIS and GLC2000. These characteristics of spatial patterns and quantitative agree- ment could be partly explained by the complex landscapes, mixed vegetation, low separability of spectro-temporal- texture signals, and coarse pixels. The differences of class definition among different the classification schemes also affects the agreement. Each product had its advantages and limitations, but neither the overall accuracy nor the class-specific accuracy could meet the requirements of climate modeling.

  10. Estimating ground-level PM_{2.5} concentrations over three megalopolises in China using satellite-derived aerosol optical depth measurements

    Science.gov (United States)

    Zheng, Yixuan; Zhang, Qiang; Liu, Yang; Geng, Guannan; He, Kebin

    2016-04-01

    Numerous previous studies have revealed that statistical models which combine satellite-derived aerosol optical depth (AOD) and PM2.5 measurements acquired at scattered monitoring sites provide an effective method for deriving continuous spatial distributions of ground-level PM2.5 concentrations. Using the national monitoring networks that have recently been established by central and local governments in China, we developed linear mixed-effects (LMEs) models that integrate Moderate Resolution Imaging Spectroradiometer (MODIS) AOD measurements, meteorological parameters, and satellite-derived tropospheric NO2 column density measurements as predictors to estimate PM2.5 concentrations over three major industrialized regions in China, namely, the Beijing-Tianjin-Hebei region (BTH), the Yangtze River Delta region (YRD), and the Pearl River Delta region (PRD). The models developed for these three regions exploited different predictors to account for their varying topographies and meteorological conditions. Considering the importance of unbiased PM2.5 predictions for epidemiological studies, the correction factors calculated from the surface PM2.5 measurements were applied to correct biases in the predicted annual average PM2.5 concentrations introduced by non-stochastic missing AOD measurements. Leave-one-out cross-validation (LOOCV) was used to quantify the accuracy of our models. Cross-validation of the daily predictions yielded R2 values of 0.77, 0.8 and 0.8 and normalized mean error (NME) values of 22.4%, 17.8% and 15.2% for BTH, YRD and PRD, respectively. For the annual average PM2.5 concentrations, the LOOCV R2 values were 0.85, 0.76 and 0.71 for the three regions, respectively, whereas the LOOCV NME values were 8.0%, 6.9% and 8.4%, respectively. We found that the incorporation of satellite-based NO2 column density into the LMEs model contribute to considerable improvements in annual prediction accuracy for both BTH and YRD. The satisfactory performance of our

  11. Normalized difference vegetation index (NDVI) variation among cultivars and environments

    Science.gov (United States)

    Although Nitrogen (N) is an essential nutrient for crop production, large preplant applications of fertilizer N can result in off-field loss that causes environmental concerns. Canopy reflectance is being investigated for use in variable rate (VR) N management. Normalized difference vegetation index...

  12. Influence of satellite-derived photolysis rates and NOx emissions on Texas ozone modeling

    Directory of Open Access Journals (Sweden)

    W. Tang

    2014-09-01

    Full Text Available Uncertain photolysis rates and emission inventory impair the accuracy of state-level ozone (O3 regulatory modeling. Past studies have separately used satellite-observed clouds to correct the model-predicted photolysis rates, or satellite-constrained top-down NOx emissions to identify and reduce uncertainties in bottom-up NOx emissions. However, the joint application of multiple satellite-derived model inputs to improve O3 State Implementation Plan (SIP modeling has rarely been explored. In this study, Geostationary Operational Environmental Satellite (GOES observations of clouds are applied to derive the photolysis rates, replacing those used in Texas SIP modeling. This changes modeled O3 concentrations by up to 80 ppb and improves O3 simulations by reducing modeled normalized mean bias (NMB and normalized mean error (NME by up to 0.1. A sector-based discrete Kalman filter (DKF inversion approach is incorporated with the Comprehensive Air Quality Model with extensions (CAMx-Decoupled Direct Method (DDM model to adjust Texas NOx emissions using a high resolution Ozone Monitoring Instrument (OMI NO2 product. The discrepancy between OMI and CAMx NO2 vertical column densities (VCD is further reduced by increasing modeled NOx lifetime and adding an artificial amount of NO2 in the upper troposphere. The sector-based DKF inversion tends to scale down area and non-road NOx emissions by 50%, leading to a 2–5 ppb decrease in ground 8 h O3 predictions. Model performance in simulating ground NO2 and O3 are improved using inverted NOx emissions, with 0.25 and 0.04 reductions in NMBs and 0.13 and 0.04 reductions in NMEs, respectively. Using both GOES-derived photolysis rates and OMI-constrained NOx emissions together reduces modeled NMB and NME by 0.05 and increases the model correlation with ground measurement in O3 simulations and makes O3 more sensitive to NOx emissions in the O3 non-attainment areas.

  13. Utilizing Satellite-derived Precipitation Products in Hydrometeorological Applications

    Science.gov (United States)

    Liu, Z.; Ostrenga, D.; Teng, W. L.; Kempler, S. J.; Huffman, G. J.

    2012-12-01

    via OPeNDAP (http://disc.sci.gsfc.nasa.gov/services/opendap/) that can be used for remote access to individual variables within datasets in a form usable by many tools, such as IDV, McIDAS-V, Panoply, Ferret and GrADS; 4) GrADS-DODS Data Server or GDS (http://disc2.nascom.nasa.gov/dods/); 5) The Open Geospatial Consortium (OGC) Web Map Service (WMS) (http://disc.sci.gsfc.nasa.gov/services/wxs_ogc.shtml) that allows the use of data and enables clients to build customized maps with data coming from a different network; and 6) Providing NASA gridded hydrological data access through CUAHSI HIS (Consortium of Universities for the Advancement of Hydrologic Science, Inc. - Hydrologic Information Systems).

  14. Using GIS data and satellite derived irradiance to optimize siting of PV installations in Switzerland

    Science.gov (United States)

    Kahl, Annelen; Nguyen, Viet-Anh; Bartlett, Stuart; Sossan, Fabrizio; Lehning, Michael

    2016-04-01

    For a successful distribution strategy of PV installations, it does not suffice to choose the locations with highest annual total irradiance. Attention needs to be given to spatial correlation patterns of insolation to avoid large system-wide variations, which can cause extended deficits in supply or might even damage the electrical network. One alternative goal instead is to seek configurations that provide the smoothest energy production, with the most reliable and predictable supply. Our work investigates several scenarios, each pursuing a different strategy for a future renewable Switzerland without nuclear power. Based on an estimate for necessary installed capacity for solar power [Bartlett, 2015] we first use heuristics to pre-select realistic placements for PV installations. Then we apply optimization methods to find a subset of locations that provides the best possible combined electricity production. For the first part of the selection process, we use a DEM to exclude high elevation zones which would be difficult to access and which are prone to natural hazards. Then we use land surface cover information to find all zones with potential roof area, deemed suitable for installation of solar panels. The optimization employs Principal Component Analysis of satellite derived irradiance data (Surface Incoming Shortwave Radiation (SIS), based on Meteosat Second Generation sensors) to incorporate a spatial aspect into the selection process that does not simply maximize annual total production but rather provides the most robust supply, by combining regions with anti-correlated cloud cover patterns. Depending on the initial assumptions and constraints, the resulting distribution schemes for PV installations vary with respect to required surface area, annual total and lowest short-term production, and illustrate how important it is to clearly define priorities and policies for a future renewable Switzerland.

  15. Interindividual differences in H reflex modulation during normal walking

    DEFF Research Database (Denmark)

    Simonsen, Erik B; Dyhre-Poulsen, Poul; Alkjaer, T

    2002-01-01

    Based on previous studies, at least two different types of soleus Hoffmann (H) reflex modulation were likely to be found during normal human walking. Accordingly, the aim of the present study was to identify different patterns of modulation of the soleus H reflex and to examine whether...... or not subjects with different H reflex modulation would exhibit different walking mechanics and different EMG activity. Fifteen subjects walked across two force platforms at 4.5 km/h (+/-10%) while the movements were recorded on video. The soleus H reflex and EMG activity were recorded separately during...... treadmill walking at 4.5 km/h. Using a two-dimensional analysis joint angles, angular velocities, accelerations, linear velocities and accelerations were calculated, and net joint moments about the ankle, knee and hip joint were computed by inverse dynamics from the video and force plate data. Six subjects...

  16. Handling of subpixel structures in the application of satellite derived irradiance data for solar energy system analysis - a review

    Science.gov (United States)

    Beyer, Hans Georg

    2016-04-01

    With the increasing availability of satellite derived irradiance information, this type of data set is more and more in use for the design and operation of solar energy systems, most notably PV- and CSP-systems. By this, the need for data measured on-site is reduced. However, due to basic limitations of the satellite-derived data, several requirements put by the intended application cannot be coped with this data type directly. Traw satellite information has to be enhanced in both space and time resolution by additional information to be fully applicable for all aspects of the modelling od solar energy systems. To cope with this problem, several individual and collaborative projects had been performed in the recent years or are ongoing. Approaches are on one hand based on pasting synthesized high-resolution data into the low-resolution original sets. Pre-requite is an appropriate model, validated against real world data. For the case of irradiance data, these models can be extracted either directly from ground measured data sets or from data referring to the cloud situation as gained from the images of sky cameras or from monte -carlo initialized physical models. The current models refer to the spatial structure of the cloud fields. Dynamics are imposed by moving the cloud structures according to a large scale cloud motion vector, either extracted from the dynamics interfered from consecutive satellite images or taken from a meso-scale meteorological model. Dynamic irradiance information is then derived from the cloud field structure and the cloud motion vector. This contribution, which is linked to subtask A - Solar Resource Applications for High Penetration of Solar Technologies - of IEA SHC task 46, will present the different approaches and discuss examples in view of validation, need for auxiliary information and respective general applicability.

  17. Differences in quality characteristics of normal, PSE and DFD pork.

    Science.gov (United States)

    van der Wal, P G; Bolink, A H; Merkus, G S

    1988-01-01

    Differences in water-holding capacity, colour and tenderness/toughness were studied in 21 pork loins belonging to three quality categories, i.e. dark firm dry (DFD), normal and pale soft exudative (PSE). The division into the three groups was based upon the ultimate pH-values of the loins ( 6·4). The PSE loins were characterized by a poor waterholding capacity, higher cooking loss and paler colour in contrast to the DFD category. The normal loins took an intermediate position. Neither sarcomere length, determined on fresh muscle tissue, nor Armour tenderometer values showed significant differences between the various quality categories. This was in contrast to the Warner-Bratzler (W-B) shear force values, determined on cooked loins, which had the lowest values in the DFD category. A strong relationship between the W-B values and the cooking losses was found. It was concluded that neither sarcomere length nor Armour tenderometer measurements were able to predict the shear force values of cooked pork loins.

  18. Utilization of satellite-derived estimates of meteorological and land surface characteristics in the Land Surface Model for vast agricultural region territory

    Science.gov (United States)

    Muzylev, Eugene; Startseva, Zoya; Uspensky, Alexander; Volkova, Elena

    2015-04-01

    data from named radiometers. All technologies have been adapted to the study area. Verification of the AVHRR- and MODIS-derived LST estimates has been performed through comparison with ground-measured temperatures and analogous estimates obtained from remaining sensors. The reliability of SEVIRI-derived LST estimates has been verified by comparison with similar synchronous SEVIRI-derived estimates produced in LSA SAF (Land Surface Analysis Satellite Applications Facility, Lisbon, Portugal). Correctness of LAI estimates has been confirmed by comparing time behavior of satellite- and ground-based LAI during vegetation season. Satellite-derived estimates of precipitation have been built using the Multi Threshold Method (MTM) developed for automatic pixel-by-pixel classification of AVHRR and SEVIRI data. The method is intended for the cloud detection and identification of its types, estimation of the maximum liquid water content and water content of the cloud layer, allocation of precipitation zones and determination of instantaneous maximum intensities of precipitation in the pixel range around the clock throughout the year independently of the land surface type. Measurement data from five AVHRR channels or from eleven SEVIRI channels as well as their differences have been used in the MTM as predictors. To validate the methodology, ground-based observation data on daily precipitation sums at agricultural meteorological stations of the study region have been used. The probability of correct precipitation zone detection from satellite data is at least 70% (80-85% in some cases) when compared with ground-based observations. In the frame of this approach the transition from the rainfall intensity estimation to the calculation of their daily values has been accomplished. In the study the AVHRR- and SEVIRI-derived daily, monthly and annual sums of precipitation for the region of interest have been calculated. The daily and monthly sums have been found to be in good agreement

  19. Bi-directional normalized difference vegetation index: concept and application

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The data products of land surface bi-directional reflectance distribution function (BRDF) from the space-borne Polarization and Directionality of the Earth Reflectance (POLDER) of France and the Moderate Resolution Imaging Spectroradiometer (MODIS)of USA are available recently, but the atmospheric correction for meeting the requirement of quantitative remote sensing is still a very dfficult problem. This paper presents a concept: bi-directional normalized difference vegetation index (Bi-NDVI), in order to consider simul taneously the effects of both land surface BRDF and atmospheric path scattering. An atmospheric quality index is thus defined for satellite multi-angular observations. The quality of MODIS/BRDF data products can be improved notably through iterative inversion weighed by this index.

  20. Normalization vs. Social Role Valorization: Similar or Different?

    Science.gov (United States)

    Kumar, Akhilesh; Singh, Rajani Ranjan; Thressiakutty, A. T.

    2015-01-01

    The radical changes towards services for persons with disabilities were brought by Principle of Normalization, originated in 1969. As a consequence of Normalization, disability as a whole, and intellectual disability in particular, received the attention of the masses and the intelligentsia began advocating normalization ideologies which became…

  1. Interindividual differences in H reflex modulation during normal walking.

    Science.gov (United States)

    Simonsen, Erik B; Dyhre-Poulsen, Poul; Alkjaer, Tine; Aagaard, Per; Magnusson, S Peter

    2002-01-01

    Based on previous studies, at least two different types of soleus Hoffmann (H) reflex modulation were likely to be found during normal human walking. Accordingly, the aim of the present study was to identify different patterns of modulation of the soleus H reflex and to examine whether or not subjects with different H reflex modulation would exhibit different walking mechanics and different EMG activity. Fifteen subjects walked across two force platforms at 4.5 km/h (+/-10%) while the movements were recorded on video. The soleus H reflex and EMG activity were recorded separately during treadmill walking at 4.5 km/h. Using a two-dimensional analysis joint angles, angular velocities, accelerations, linear velocities and accelerations were calculated, and net joint moments about the ankle, knee and hip joint were computed by inverse dynamics from the video and force plate data. Six subjects (group S) showed a suppressed H reflex during the swing phase, and 9 subjects (group LS) showed increasing reflex excitability during the swing phase. The plantar flexor dominated moment about the ankle joint was greater for group LS. In contrast, the extensor dominated moment about the knee joint was greater for the S group. The hip joint moment was similar for the groups. The EMG activity in the vastus lateralis and anterior tibial muscles was greater prior to heel strike for the S group. These data indicate that human walking exhibits at least two different motor patterns as evaluated by gating of afferent input to the spinal cord, by EMG activity and by walking mechanics. Increasing H reflex excitability during the swing phase appears to protect the subject against unexpected perturbations around heel strike by a facilitated stretch reflex in the triceps surae muscle. Alternatively, in subjects with a suppressed H reflex in the swing phase the knee joint extensors seem to form the primary protection around heel strike.

  2. a Diagnostic Approach to Obtaining Planetary Boundary Layer Winds Using Satellite-Derived Thermal Data

    Science.gov (United States)

    Belt, Carol Lynn

    The feasibility of using satellite-derived thermal data to generate realistic synoptic-scale winds within the planetary boundary layer (PBL) is examined. Diagnostic "modified Ekman" wind equations from the Air Force Global Weather Central (AFGWC) Boundary Layer Model are used to compute winds at seven levels within the PBL transition layer (50 m to 1600 m AGL). Satellite-derived winds based on 62 predawn (0921 GMT 19 April 1979) TIROS-N soundings are compared to similarly-derived wind fields based on 39 AVE-SESAME II rawinsonde (RAOB) soundings taken 2 h later. Actual wind fields are also used as a basis for comparison. Qualitative and statistical comparisons show that the Ekman winds from both sources are in very close agreement, with an average vector correlation coefficient of 0.815. Best results are obtained at 300 m AGL. Satellite winds tend to be slightly weaker than their RAOB counterparts and exhibit a greater degree of cross-isobaric flow. The modified Ekman winds show a significant improvement over geostrophic values at levels nearest the surface. Horizontal moisture divergence, moisture advection, velocity divergence and relative vorticity are computed at 300 m AGL using satellite-derived winds and moisture data. Results show excellent agreement with corresponding RAOB-derived values. Areas of horizontal moisture convergence, velocity convergence, and positive vorticity are nearly coincident and align in regions which later develop intense convection. Vertical motion at 1600 m AGL is computed using stepwise integration of the satellite winds through the PBL. Values and patterns are similar to those obtained using the RAOB-derived winds. Regions of maximum upward motion correspond with areas of greatest moisture convergence and the convection that later develops.

  3. Asymptotic normality of recursive algorithms via martingale difference arrays

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    Werner Schachinger

    2001-12-01

    Full Text Available We propose martingale central limit theorems as an tool to prove asymptotic normality of the costs of certain recursive algorithms which are subjected to random input data. The recursive algorithms that we have in mind are such that if input data of size N produce random costs L N, then L N = D L n + L N-n +R N for N ≥ n 0 ≥2, where n follows a certain distribution P N on the integers {0, … ,N} and L k = D L k for k≥0. L n, L N-n and R N are independent, conditional on n, and R N are random variables, which may also depend on n, corresponding to the cost of splitting the input data of size N (into subsets of size n and N-n and combining the results of the recursive calls to yield the overall result. We construct a martingale difference array with rows converging to Z N:= [L N- EL N ] / [√ Var L N ]. Under certain compatibility assumptions on the sequence (P N N≥0 we show that a pair of sufficient conditions (of Lyapunov type for Z N → D N(0,1 can be restated as a pair of conditions regarding asymptotic relations between three sequences. All these sequences satisfy the same type of linear equation, that is also the defining equation for the sequence (EL N N≥0 and thus very likely a well studied object. In the case that the P N are binomial distributions with the same parameter p, and for deterministic R N, we demonstrate the power of this approach. We derive very general sufficient conditions in terms of the sequence (R N N≥0 (and for the scale R N =N α a characterization of those α leading to asymptotic normality of Z N.

  4. Investigation of different normalization reactions for dp collisions at ANKE

    Energy Technology Data Exchange (ETDEWEB)

    Fritzsch, Christopher; Guderian, Daniel; Mielke, Malte; Papenbrock, Michael; Schroeer, Daniel; Khoukaz, Alfons [Institut fuer Kernphysik, Westfaelische Wilhelms-Universitaet Muenster (Germany)

    2015-07-01

    Studies on the total cross sections of the reaction d+p → {sup 3}He + η are of special interest since they differ strongly from a pure phase space behaviour near threshold. This behaviour could be an indication for the presence of a quasi bound state of the η{sup 3}He-system. New high precision data from the ANKE spectrometer at the accelerator ring COSY at the Forschungszentrum Juelich allow the extraction of precise total and differential cross section values for the η production up to an excess energy of Q=15 MeV. Therefore, a careful luminosity determination was realized via dp-elastic scattering for 18 beam momenta in a range between 3120.17 MeV/c ≤ p{sub d} ≤ 3204.16 MeV/c. Furthermore, to verify the results of the dp-elastic scattering an independent normalization channel d+p → d+π{sup 0} + p{sub spec} was used. The method and recent results for both channels are presented and discussed.

  5. Application of satellite derived information for disaster risk reduction: vulnerability assessment for southwest coast of Pakistan

    Science.gov (United States)

    Rafiq, Lubna; Blaschke, Thomas; Zeil, Peter

    2010-10-01

    The SW-coast of Pakistan is vulnerable to natural disasters, such as cyclones and tsunamis. Lack of spatially referenced information is a major hinder for proper disaster risk management programs in Pakistan, but satellite remote sensing being reliable, fast and spatially referenced information can be used as an important component in various natural disaster risk reduction activities. This study aimed to investigate vulnerability of coastal communities to cyclone and tsunamis based on satellite derived information. It is observed that SPOT-5 is relevant source on threatened features with respect to certain vulnerabilities like road, settlements, infrastructure and used in preparation of hazard zonation and vulnerability maps. Landsat ETM found very useful in demarcation of flood inundated areas. The GIS integrated evaluation of LANDSAT and ASTER GDEM helps identify low lying areas most susceptible to flooding and inundation by cyclone surges and tsunamis. The GIS integrated evaluation of SPOT, LANDSAT and ASTER GDEM data helps identify areas and infrastructure most vulnerable to cyclone surges and tsunami. Additionally, analysis of the vulnerability of critical infrastructures (schools, hospitals) within hazard zones provides indicators for the degree of spatial exposure to disaster. Satellite derived information in conjunction with detailed surveys of hazard prone areas can provide comprehensive vulnerability and risk analysis.

  6. Evaluating satellite-derived long-term historical precipitation datasets for drought monitoring in Chile

    Science.gov (United States)

    Zambrano, Francisco; Wardlow, Brian; Tadesse, Tsegaye

    2016-10-01

    Precipitation is a key parameter for the study of climate change and variability and the detection and monitoring of natural disaster such as drought. Precipitation datasets that accurately capture the amount and spatial variability of rainfall is critical for drought monitoring and a wide range of other climate applications. This is challenging in many parts of the world, which often have a limited number of weather stations and/or historical data records. Satellite-derived precipitation products offer a viable alternative with several remotely sensed precipitation datasets now available with long historical data records (+30 years), which include the Climate Hazards Group InfraRed Precipitation with Station (CHIRPS) and Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks-Climate Data Record (PERSIANN-CDR) datasets. This study presents a comparative analysis of three historical satellite-based precipitation datasets that include Tropical Rainfall Measuring Mission (TRMM) Multi-satellite Precipitation Analysis (TMPA) 3B43 version 7 (1998-2015), PERSIANN-CDR (1983-2015) and CHIRPS 2.0 (1981-2015) over Chile to assess their performance across the country and evaluate their applicability for agricultural drought evaluation when used in the calculation of commonly used drought indicator as the Standardized Precipitation Index (SPI). In this analysis, 278 weather stations of in-situ rainfall measurements across Chile were initially compared to the satellite-based precipitation estimates. The study area (Chile) was divided into five latitudinal zones: North, North-Central, Central, South-Central and South to determine if there were a regional difference among these satellite-based estimates. Nine statistics were used to evaluate the performance of satellite products to estimate the amount and spatial distribution of historical rainfall across Chile. Hierarchical cluster analysis, k-means and singular value decomposition were used to

  7. Evaluating satellite-derived long-term historical precipitation datasets for drought monitoring in Chile

    Science.gov (United States)

    Zambrano, Francisco; Wardlow, Brian; Tadesse, Tsegaye; Lillo-Saavedra, Mario; Lagos, Octavio

    2017-04-01

    Precipitation is a key parameter for the study of climate change and variability and the detection and monitoring of natural disaster such as drought. Precipitation datasets that accurately capture the amount and spatial variability of rainfall is critical for drought monitoring and a wide range of other climate applications. This is challenging in many parts of the world, which often have a limited number of weather stations and/or historical data records. Satellite-derived precipitation products offer a viable alternative with several remotely sensed precipitation datasets now available with long historical data records (+30years), which include the Climate Hazards Group InfraRed Precipitation with Station (CHIRPS) and Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks-Climate Data Record (PERSIANN-CDR) datasets. This study presents a comparative analysis of three historical satellite-based precipitation datasets that include Tropical Rainfall Measuring Mission (TRMM) Multi-satellite Precipitation Analysis (TMPA) 3B43 version 7 (1998-2015), PERSIANN-CDR (1983-2015) and CHIRPS 2.0 (1981-2015) over Chile to assess their performance across the country and for the case of the two long-term products the applicability for agricultural drought were evaluated when used in the calculation of commonly used drought indicator as the Standardized Precipitation Index (SPI). In this analysis, 278 weather stations of in situ rainfall measurements across Chile were initially compared to the satellite data. The study area (Chile) was divided into five latitudinal zones: North, North-Central, Central, South-Central and South to determine if there were a regional difference among these satellite products, and nine statistics were used to evaluate their performance to estimate the amount and spatial distribution of historical rainfall across Chile. Hierarchical cluster analysis, k-means and singular value decomposition were used to analyze

  8. Advanced Very High Resolution Radiometer Normalized Difference Vegetation Index Composites

    Science.gov (United States)

    ,

    2005-01-01

    The Advanced Very High Resolution Radiometer (AVHRR) is a broad-band scanner with four to six bands, depending on the model. The AVHRR senses in the visible, near-, middle-, and thermal- infrared portions of the electromagnetic spectrum. This sensor is carried on a series of National Oceanic and Atmospheric Administration (NOAA) Polar Orbiting Environmental Satellites (POES), beginning with the Television InfraRed Observation Satellite (TIROS-N) in 1978. Since 1989, the United States Geological Survey (USGS) Center for Earth Resources Observation and Science (EROS) has been mapping the vegetation condition of the United States and Alaska using satellite information from the AVHRR sensor. The vegetation condition composites, more commonly called greenness maps, are produced every week using the latest information on the growth and condition of the vegetation. One of the most important aspects of USGS greenness mapping is the historical archive of information dating back to 1989. This historical stretch of information has allowed the USGS to determine a 'normal' vegetation condition. As a result, it is possible to compare the current week's vegetation condition with normal vegetation conditions. An above normal condition could indicate wetter or warmer than normal conditions, while a below normal condition could indicate colder or dryer than normal conditions. The interpretation of departure from normal will depend on the season and geography of a region.

  9. A Satellite-Derived Upper-Tropospheric Water Vapor Transport Index for Climate Studies

    Science.gov (United States)

    Jedlovec, Gray J.; Lerner, Jeffrey A.; Atkinson, Robert J.

    1998-01-01

    A new approach is presented to quantify upper-level moisture transport from geostationary satellite data. Daily time sequences of Geostationary Operational Environmental Satellite GOES-7 water vapor imagery were used to produce estimates of winds and water vapor mixing ratio in the cloud-free region of the upper troposphere sensed by the 6.7- microns water vapor channel. The winds and mixing ratio values were gridded and then combined to produce a parameter called the water vapor transport index (WVTI), which represents the magnitude of the two-dimensional transport of water vapor in the upper troposphere. Daily grids of WVTI, meridional moisture transport, mixing ratio, pressure, and other associated parameters were averaged to produce monthly fields for June, July, and August (JJA) of 1987 and 1988 over the Americas and surrounding oceanic regions, The WVTI was used to compare upper-tropospheric moisture transport between the summers of 1987 and 1988, contrasting the latter part of the 1986/87 El Nino event and the La Nina period of 1988. A similar product derived from the National Centers for Environmental Prediction (NCEP)-National Center for Atmospheric Research (NCAR) 40-Year Reanalysis Project was used to help to validate the index. Although the goal of this research was to describe the formulation and utility of the WVTI, considerable insight was obtained into the interannual variability of upper-level water vapor transport. Both datasets showed large upper-level water vapor transport associated with synoptic features over the Americas and with outflow from tropical convective systems. Minimal transport occurred over tropical and subtropical high pressure regions where winds were light. Index values from NCEP-NCAR were 2-3 times larger than that determined from GOES. This difference resulted from large zonal wind differences and an apparent overestimate of upper-tropospheric moisture in the reanalysis model. A comparison of the satellite-derived monthly

  10. Spatial Correlation of Satellite-Derived PM2.5 with Hospital Admissions for Respiratory Diseases

    Directory of Open Access Journals (Sweden)

    Ching-Ju Liu

    2016-11-01

    Full Text Available Respiratory diseases, particularly allergic rhinitis, are spatially and temporally correlated with the ground PM2.5 level. A study of the correlation between the two factors should therefore account for spatiotemporal variations. Satellite observation has the advantage of wide spatial coverage over pin-point style ground-based in situ monitoring stations. Therefore, the current study used both ground measurement and satellite data sets to investigate the spatial and temporal correlation of satellite-derived PM2.5 with respiratory diseases. This study used 4-year satellite data and PM2.5 levels of the period at eight stations in Taiwan to obtain the spatial and temporal relationship between aerosol optical depth (AOD and PM2.5. The AOD-PM2.5 model was further examined using the cross-validation (CV technique and was found to have high reliability compared with similar models. The model was used to obtain satellite-derived PM2.5 levels and to analyze the hospital admissions for allergic rhinitis in 2008. The results suggest that adults (18–65 years and children (3–18 years are the most vulnerable groups to the effect of PM2.5 compared with infants and elderly people. This result may be because the two affected age groups spend longer time outdoors. This result may also be attributed to the long-range PM2.5 transport from upper stream locations and the atmospheric circulation patterns, which are significant in spring and fall. The results of the current study suggest that additional environmental factors that might be associated with respiratory diseases should be considered in future studies.

  11. Can satellite-derived water surface changes be used to calibrate a hydrodynamic model?

    Science.gov (United States)

    Revilla-Romero, Beatriz; Beck, Hylke; Salamon, Peter; Burek, Peter; de Roo, Ad; Thielen, Jutta

    2015-04-01

    The limited availability of recent ground observational data is one of the main challenges for validation of hydrodynamic models. This is especially relevant for real-time global applications such as flood forecasting models. In this study, we aim to use remotely-sensed data from the Global Flood Detection System (GFDS) as a proxy of river discharge time series and test its value through calibration of the hydrological model LISFLOOD. This was carried out for the time period 1998-2010 at 40 sites in Africa, Europe, North America and South America by calibrating the parameters that control the flow routing and groundwater processes. We compared the performance of the calibrated simulated discharge time series that used satellite-derived data with the ground discharge time series. Furthermore, we compared it with the independent calibrated run that used ground data and also, to the non-calibrated simulated discharge time series. The non-calibrated set up used a set of parameters which values were predefined by expert-knowledge. This is currently being used by the LISFLOOD set up model embedded in the pre-operational Global Flood Awareness System (GloFAS). The results of this study showed that the satellite surface water changes from the Global Flood Detection System can be used as a proxy of river discharge data, through the demonstration of its added value for model calibration and validation. Using satellite-derived data, the skill scores obtained by the calibrated simulated model discharge improved when comparing to non-calibrated simulated time series. Calibration, post-processing and data assimilation strategies of satellite data as a proxy for streamflow data within the global hydrological model are outlined and discussed.

  12. Surface radiation at sea validation of satellite-derived data with shipboard measurements

    Directory of Open Access Journals (Sweden)

    Hein Dieter Behr

    2009-03-01

    Full Text Available Quality-controlled and validated radiation products are the basis for their ability to serve the climate and solar energy community. Satellite-derived radiation fluxes are well preferred for this task as they cover the whole research area in time and space. In order to monitor the accuracy of these data, validation with well maintained and calibrated ground based measurements is necessary. Over sea, however, long-term accurate reference data sets from calibrated instruments recording radiation are scarce. Therefore data from research vessels operating at sea are used to perform a reasonable validation. A prerequisite is that the instruments on board are maintained as well as land borne stations. This paper focuses on the comparison of radiation data recorded on board of the German Research Vessel "Meteor" during her 13 months cruise across the Mediterranean and the Black Sea with CM-SAF products using NOAA- and MSG-data (August 2006-August 2007: surface incoming short-wave radiation (SIS and surface downward long-wave radiation (SDL. Measuring radiation fluxes at sea causes inevitable errors, e.g.shadowing of fields of view of the radiometers by parts of the ship. These ship-inherent difficulties are discussed at first. A comparison of pairs of ship-recorded and satellite-derived mean fluxes for the complete measuring period delivers a good agreement: the mean bias deviation (MBD for SIS daily means is −7.6 W/m2 with a median bias of −4 W/m2 and consistently the MBD for monthly means is −7.3 W/m2, for SDL daily means the MBD is 8.1 and 6 W/m2 median bias respectively. The MBD for monthly means is 8.2 W/m2. The variances of the daily means (ship and satellite have the same annual courses for both fluxes. No significant dependence of the bias on the total cloud cover recorded according to WMO (1969 has been found. The results of the comparison between ship-based observations and satellite retrieved surface radiation reveal the good accuracy

  13. Model-simulated and Satellite-derived Leaf Area Index (LAI) Comparisons Across Multiple Spatial Scales

    Science.gov (United States)

    Iiames, J. S., Jr.; Cooter, E. J.

    2016-12-01

    Leaf Area Index (LAI) is an important parameter in assessing vegetation structure for characterizing forest canopies over large areas at broad spatial scales using satellite remote sensing data. However, satellite-derived LAI products can be limited by obstructed atmospheric conditions yielding sub-optimal values, or complete non-returns. The United States Environmental Protection Agency's Exposure Methods and Measurements and Computational Exposure Divisions are investigating the viability of supplemental modelled LAI inputs into satellite-derived data streams to support various regional and local scale air quality models for retrospective and future climate assessments. In this present study, one-year (2002) of plot level stand characteristics at four study sites located in Virginia and North Carolina (USA) are used to calibrate species-specific plant parameters in a semi-empirical biogeochemical model. The Environmental Policy Integrated Climate (EPIC) model was designed primarily for managed agricultural field crop ecosystems, but also includes managed woody species that span both xeric and mesic sites (e.g., mesquite, pine, oak, etc.). LAI was simulated using EPIC at a 4 km2 and 12 km2 grid coincident with the regional Community Multiscale Air Quality Model (CMAQ) grid. LAI comparisons were made between model-simulated and MODIS-derived LAI. Field/satellite-upscaled LAI was also compared to the corresponding MODIS LAI value. Preliminary results show field/satellite-upscaled LAI (1 km2) was 1.5 to 3 times smaller than that with the corresponding 1 km2 MODIS LAI for all four sites across all dates, with the largest discrepancies occurring at leaf-out and leaf senescence periods. Simulated LAI/MODIS LAI comparison results will be presented at the conference. Disclaimer: This work is done in support of EPA's Sustainable Healthy Communities Research Program. The U.S. Environmental Protection Agency funded and conducted the research described in this paper. Although

  14. High resolution satellite derived erodibility factors for WRF/Chem windblown dust simulations in Argentina

    Science.gov (United States)

    Cremades, Pablo Gabriel; Fernandez, Rafael Pedro; Allend, David; Mulena, Celeste; Puliafito, Salvador Enrique

    2017-04-01

    A proper representation of dust sources is critical to accurately predict atmospheric particle concentrations in regional windblown dust simulations. The Weather Research and Forecasting model with Chemistry (WRF/Chem) includes a topographic-based erodibility map originally conceived for global scale modeling, which fails to identify the geographical location of dust sources in many regions of Argentina. Therefore, this study aims at developing a method to obtain a high-resolution erodibility map suitable for regional or local scale modeling using WRF/Chem. We present two independent approaches based on global methods to estimate soil erodibility using satellite retrievals, i.e. topography from the Shuttle Radar Topography Mission (SRTM) and surface reflectance from the Moderate Resolution Imaging Spectroradiometer (MODIS). Simulation results of a severe Zonda wind episode in the arid central-west Argentina serve as bases for the analysis of these methods. Simulated dust concentration at surface level is compared with particulate matter measurements at one site in Mendoza city. In addition, we use satellite aerosol optical depth (AOD) retrievals to investigate model performance in reproducing spatial distribution of dust emissions. The erodibility map based on surface reflectance from MODIS improves the representation of small scale features, and increases the overall dust aerosol loading with respect to the standard map included by default. Simulated concentrations are in good agreement with measurements as well as satellite derived dust spatial distribution.

  15. Comparative Study of Ground Measured, Satellite-Derived, and Estimated Global Solar Radiation Data in Nigeria

    Directory of Open Access Journals (Sweden)

    Boluwaji M. Olomiyesan

    2016-01-01

    Full Text Available In this study, the performance of three global solar radiation models and the accuracy of global solar radiation data derived from three sources were compared. Twenty-two years (1984–2005 of surface meteorological data consisting of monthly mean daily sunshine duration, minimum and maximum temperatures, and global solar radiation collected from the Nigerian Meteorological (NIMET Agency, Oshodi, Lagos, and the National Aeronautics Space Agency (NASA for three locations in North-Western region of Nigeria were used. A new model incorporating Garcia model into Angstrom-Prescott model was proposed for estimating global radiation in Nigeria. The performances of the models used were determined by using mean bias error (MBE, mean percentage error (MPE, root mean square error (RMSE, and coefficient of determination (R2. Based on the statistical error indices, the proposed model was found to have the best accuracy with the least RMSE values (0.376 for Sokoto, 0.463 for Kaduna, and 0.449 for Kano and highest coefficient of determination, R2 values of 0.922, 0.938, and 0.961 for Sokoto, Kano, and Kaduna, respectively. Also, the comparative study result indicates that the estimated global radiation from the proposed model has a better error range and fits the ground measured data better than the satellite-derived data.

  16. Validation of Satellite-Derived Sea Surface Temperatures for Waters around Taiwan

    Directory of Open Access Journals (Sweden)

    Ming-An Lee

    2005-01-01

    Full Text Available In order to validate the Advanced Very High Resolution Radiometer (AVHRR-derived sea surface temperatures (SST of the waters around Taiwan, we generated a match-up data set of 961 pairs, which included in situ SSTs and concurrent AVHRR measurements for the period of 1998 to 2002. Availability of cloud-free images, i.e., images with more than 85% of cloud-free area in their coverage, was about 2.23% of all AVHRR images during the study period. The range of in situ SSTs was from _ to _ The satellite derived-SSTs through MCSST and NLSST algorithms were linearly related to the in situ SSTs with correlation coefficients of 0.985 and 0.98, respectively. The MCSSTs and NLSSTs had small biases of 0.009 _ and 0.256 _ with root mean square deviations of 0.64 _ and 0.801 _ respectively, therefore the AVHRR-based MCSSTs and NLSSTs had high accuracy in the seas around Taiwan.

  17. Evaluation of a physically-based snow model with infrared and microwave satellite-derived estimates

    Science.gov (United States)

    Wang, L.

    2013-05-01

    Snow (with high albedo, as well as low roughness and thermal conductivity) has significant influence on the land-atmosphere interactions in the cold climate and regions of high elevation. The spatial and temporal variability of the snow distribution on a basin scale greatly determines the timing and magnitude of spring snowmelt runoff. For improved water resources management, a physically-based distributed snow model has been developed and applied to the upper Yellow River Basin to provide the outputs of snow variables as well as streamflows from 2001 to 2005. Remotely-sensed infrared information from MODIS satellites has been used to evaluate the model's outputs of spatially-distributed snow cover extent (SCE) and land surface temperature (LST); while the simulated snow depth (SD) and snow water equivalent (SWE) have been compared with the microwave information from SSM/I and AMSR-E satellites. In general, the simulated streamflows (including spring snowmelt) agree fairly well with the gauge-based observations; while the modeled snow variables show acceptable accuracies through comparing to various satellite-derived estimates from infrared or microwave information.;

  18. Satellite derived precipitation and freshwater flux variability and its dependence on the North Atlantic Oscillation

    Science.gov (United States)

    Andersson, Axel; Bakan, Stephan; Graßl, Hartmut

    2010-08-01

    The variability of satellite retrieved precipitation and freshwater flux from the `Hamburg Ocean Atmosphere Parameters and Fluxes from Satellite Data' (HOAPS) is assessed with special emphasis on the `North Atlantic Oscillation' (NAO). To cover also land areas, a novel combination of the satellite derived precipitation climatology with the rain gauge based `Full Data Reanalysis Product Version 4', of the `Global Precipitation Climatology Centre' (GPCC) is used. This yields unique high-resolution, quasi-global precipitation fields compiled from two independent data sources. Over the ocean, the response of the freshwater balance and the related parameters to the NAO is investigated for the first time by using a purely satellite based data set. A strong dependence of precipitation patterns to the state of the NAO is found. On synoptic scale this is in accordance with earlier findings by other satellite based and reanalysis products. Furthermore, the consistency of the combined HOAPS-3/GPCC data set allows also detailed regional analyses of precipitation patterns. The response of HOAPS-3 freshwater flux to the NAO is dominated by precipitation at mid and high latitudes, while for the subtropical regions the feedback of the evaporation is stronger.

  19. Sex differences in cognitive trajectories in clinically normal older adults.

    Science.gov (United States)

    McCarrey, Anna C; An, Yang; Kitner-Triolo, Melissa H; Ferrucci, Luigi; Resnick, Susan M

    2016-03-01

    Age effects on cognitive functioning are well-documented, but effects of sex on trajectories of cognitive aging are less clear. We examined cognitive ability across a variety of measures for 1,065 to 2,127 participants (mean baseline age 64.1 to 69.7 years) from the Baltimore Longitudinal Study of Aging who were repeatedly tested over a mean follow-up interval of 3.0 to 9.0 years with a mean of 2.3 to 4.4 assessments. Memory and other cognitive tests were administered at each visit, assessing mental status, verbal learning and memory, figural memory, language, attention, perceptuomotor speed and integration, executive function, and visuospatial ability. Importantly, participants free from cognitive impairment at all time points were used in the analyses. Results showed that for all tests, higher age at baseline was significantly associated with lower scores, and performance declined over time. In addition, advancing age was associated with accelerated longitudinal declines in performance (trend for mental status). After adjusting for age, education, and race, sex differences were observed across most tests of specific cognitive abilities examined. At baseline, males outperformed females on the 2 tasks of visuospatial ability, and females outperformed males in most other tests of cognition. Sex differences in cognitive change over time indicated steeper rates of decline for men on measures of mental status, perceptuomotor speed and integration, and visuospatial ability, but no measures on which women showed significantly steeper declines. Our results highlight greater resilience to age-related cognitive decline in older women compared with men.

  20. Evaluating and Quantifying the Climate-Driven Interannual Variability in Global Inventory Modeling and Mapping Studies (GIMMS Normalized Difference Vegetation Index (NDVI3g at Global Scales

    Directory of Open Access Journals (Sweden)

    Alvaro Ivanoff

    2013-08-01

    Full Text Available Satellite observations of surface reflected solar radiation contain information about variability in the absorption of solar radiation by vegetation. Understanding the causes of variability is important for models that use these data to drive land surface fluxes or for benchmarking prognostic vegetation models. Here we evaluated the interannual variability in the new 30.5-year long global satellite-derived surface reflectance index data, Global Inventory Modeling and Mapping Studies normalized difference vegetation index (GIMMS NDVI3g. Pearson’s correlation and multiple linear stepwise regression analyses were applied to quantify the NDVI interannual variability driven by climate anomalies, and to evaluate the effects of potential interference (snow, aerosols and clouds on the NDVI signal. We found ecologically plausible strong controls on NDVI variability by antecedent precipitation and current monthly temperature with distinct spatial patterns. Precipitation correlations were strongest for temperate to tropical water limited herbaceous systems where in some regions and seasons > 40% of the NDVI variance could be explained by precipitation anomalies. Temperature correlations were strongest in northern mid- to high-latitudes in the spring and early summer where up to 70% of the NDVI variance was explained by temperature anomalies. We find that, in western and central North America, winter-spring precipitation determines early summer growth while more recent precipitation controls NDVI variability in late summer. In contrast, current or prior wet season precipitation anomalies were correlated with all months of NDVI in sub-tropical herbaceous vegetation. Snow, aerosols and clouds as well as unexplained phenomena still account for part of the NDVI variance despite corrections. Nevertheless, this study demonstrates that GIMMS NDVI3g represents real responses of vegetation to climate variability that are useful for global models.

  1. Evaluating and Quantifying the Climate-Driven Interannual Variability in Global Inventory Modeling and Mapping Studies (GIMMS) Normalized Difference Vegetation Index (NDVI3g) at Global Scales

    Science.gov (United States)

    Zeng, Fanwei; Collatz, George James; Pinzon, Jorge E.; Ivanoff, Alvaro

    2013-01-01

    Satellite observations of surface reflected solar radiation contain informationabout variability in the absorption of solar radiation by vegetation. Understanding thecauses of variability is important for models that use these data to drive land surface fluxesor for benchmarking prognostic vegetation models. Here we evaluated the interannualvariability in the new 30.5-year long global satellite-derived surface reflectance index data,Global Inventory Modeling and Mapping Studies normalized difference vegetation index(GIMMS NDVI3g). Pearsons correlation and multiple linear stepwise regression analyseswere applied to quantify the NDVI interannual variability driven by climate anomalies, andto evaluate the effects of potential interference (snow, aerosols and clouds) on the NDVIsignal. We found ecologically plausible strong controls on NDVI variability by antecedent precipitation and current monthly temperature with distinct spatial patterns. Precipitation correlations were strongest for temperate to tropical water limited herbaceous systemswhere in some regions and seasons 40 of the NDVI variance could be explained byprecipitation anomalies. Temperature correlations were strongest in northern mid- to-high-latitudes in the spring and early summer where up to 70 of the NDVI variance was explained by temperature anomalies. We find that, in western and central North America,winter-spring precipitation determines early summer growth while more recent precipitation controls NDVI variability in late summer. In contrast, current or prior wetseason precipitation anomalies were correlated with all months of NDVI in sub-tropical herbaceous vegetation. Snow, aerosols and clouds as well as unexplained phenomena still account for part of the NDVI variance despite corrections. Nevertheless, this study demonstrates that GIMMS NDVI3g represents real responses of vegetation to climate variability that are useful for global models.

  2. Potential of satellite-derived ecosystem functional attributes to anticipate species range shifts

    Science.gov (United States)

    Alcaraz-Segura, Domingo; Lomba, Angela; Sousa-Silva, Rita; Nieto-Lugilde, Diego; Alves, Paulo; Georges, Damien; Vicente, Joana R.; Honrado, João P.

    2017-05-01

    In a world facing rapid environmental changes, anticipating their impacts on biodiversity is of utmost relevance. Remotely-sensed Ecosystem Functional Attributes (EFAs) are promising predictors for Species Distribution Models (SDMs) by offering an early and integrative response of vegetation performance to environmental drivers. Species of high conservation concern would benefit the most from a better ability to anticipate changes in habitat suitability. Here we illustrate how yearly projections from SDMs based on EFAs could reveal short-term changes in potential habitat suitability, anticipating mid-term shifts predicted by climate-change-scenario models. We fitted two sets of SDMs for 41 plant species of conservation concern in the Iberian Peninsula: one calibrated with climate variables for baseline conditions and projected under two climate-change-scenarios (future conditions); and the other calibrated with EFAs for 2001 and projected annually from 2001 to 2013. Range shifts predicted by climate-based models for future conditions were compared to the 2001-2013 trends from EFAs-based models. Projections of EFAs-based models estimated changes (mostly contractions) in habitat suitability that anticipated, for the majority (up to 64%) of species, the mid-term shifts projected by traditional climate-change-scenario forecasting, and showed greater agreement with the business-as-usual scenario than with the sustainable-development one. This study shows how satellite-derived EFAs can be used as meaningful essential biodiversity variables in SDMs to provide early-warnings of range shifts and predictions of short-term fluctuations in suitable conditions for multiple species.

  3. Predicting bird phenology from space: satellite-derived vegetation green-up signal uncovers spatial variation in phenological synchrony between birds and their environment.

    Science.gov (United States)

    Cole, Ella F; Long, Peter R; Zelazowski, Przemyslaw; Szulkin, Marta; Sheldon, Ben C

    2015-11-01

    Population-level studies of how tit species (Parus spp.) track the changing phenology of their caterpillar food source have provided a model system allowing inference into how populations can adjust to changing climates, but are often limited because they implicitly assume all individuals experience similar environments. Ecologists are increasingly using satellite-derived data to quantify aspects of animals' environments, but so far studies examining phenology have generally done so at large spatial scales. Considering the scale at which individuals experience their environment is likely to be key if we are to understand the ecological and evolutionary processes acting on reproductive phenology within populations. Here, we use time series of satellite images, with a resolution of 240 m, to quantify spatial variation in vegetation green-up for a 385-ha mixed-deciduous woodland. Using data spanning 13 years, we demonstrate that annual population-level measures of the timing of peak abundance of winter moth larvae (Operophtera brumata) and the timing of egg laying in great tits (Parus major) and blue tits (Cyanistes caeruleus) is related to satellite-derived spring vegetation phenology. We go on to show that timing of local vegetation green-up significantly explained individual differences in tit reproductive phenology within the population, and that the degree of synchrony between bird and vegetation phenology showed marked spatial variation across the woodland. Areas of high oak tree (Quercus robur) and hazel (Corylus avellana) density showed the strongest match between remote-sensed vegetation phenology and reproductive phenology in both species. Marked within-population variation in the extent to which phenology of different trophic levels match suggests that more attention should be given to small-scale processes when exploring the causes and consequences of phenological matching. We discuss how use of remotely sensed data to study within-population variation

  4. Induction in myeloid leukemic cells of genes that are expressed in different normal tissues

    OpenAIRE

    2005-01-01

    Using DNA microarray and cluster analysis of expressed genes in a cloned line (M1-t-p53) of myeloid leukemic cells, we have analyzed the expression of genes that are preferentially expressed in different normal tissues. Clustering of 547 highly expressed genes in these leukemic cells showed 38 genes preferentially expressed in normal hematopoietic tissues and 122 other genes preferentially expressed in different normal non-hematopoietic tissues including neuronal tissues, muscle, liver and te...

  5. Different coexpressions of arthritis-relevant genes between different body organs and different brain regions in the normal mouse population.

    Science.gov (United States)

    Cao, Yanhong; Huang, Yue; Wang, Lishi; Zhu, Jiaqian; Gu, Weikuan

    2013-02-25

    Structural changes in different parts of the brain in rheumatoid arthritis (RA) patients have been reported. RA is not regarded as a brain disease. Body organs such as spleen and lung produce RA-relevant genes. We hypothesized that the structural changes in the brain are caused by changes of gene expression in body organs. Changes in different parts of the brain may be affected by altered gene expressions in different body organs. This study explored whether an association between gene expressions of an organ or a body part varies in different brain structures. By examining the association of the 10 most altered genes from a mouse model of spontaneous arthritis in a normal mouse population, we found two groups of gene expression patterns between five brain structures and spleen. The correlation patterns between the prefrontal cortex, nucleus accumbens, and spleen were similar, while the associations between the other three parts of the brain and spleen showed a different pattern. Among overall patterns of the associations between body organs and brain structures, spleen and lung had a similar pattern, and patterns for kidney and liver were similar. Analysis of the five additional known arthritis-relevant genes produced similar results. Analysis of 10 nonrelevant-arthritis genes did not result in a strong association of gene expression or clearly segregated patterns. Our data suggest that abnormal gene expressions in different diseased body organs may influence structural changes in different brain parts.

  6. SACRA – global data sets of satellite-derived crop calendars for agricultural simulations: an estimation of a high-resolution crop calendar using satellite-sensed NDVI

    Directory of Open Access Journals (Sweden)

    S. Kotsuki

    2015-01-01

    Full Text Available To date, many studies have performed numerical estimations of food production and agricultural water demand to understand the present and future supply–demand relationship. A crop calendar (CC is an essential input datum to estimate food production and agricultural water demand accurately with the numerical estimations. CC defines the date or month when farmers plant and harvest in cropland. This study aims to develop a new global data set of a satellite-derived crop calendar for agricultural simulations (SACRA and reveal advantages and disadvantages of the satellite-derived CC compared to other global products. We estimate global CC at a spatial resolution of 5 min (≈10 km using the satellite-sensed NDVI data, which corresponds well to vegetation growth and death on the land surface. We first demonstrate that SACRA shows similar spatial pattern in planting date compared to a census-based product. Moreover, SACRA reflects a variety of CC in the same administrative unit, since it uses high-resolution satellite data. However, a disadvantage is that the mixture of several crops in a grid is not considered in SACRA. We also address that the cultivation period of SACRA clearly corresponds to the time series of NDVI. Therefore, accuracy of SACRA depends on the accuracy of NDVI used for the CC estimation. Although SACRA shows different CC from a census-based product in some regions, multiple usages of the two products are useful to take into consideration the uncertainty of the CC. An advantage of SACRA compared to the census-based products is that SACRA provides not only planting/harvesting dates but also a peak date from the time series of NDVI data.

  7. Calibration of the Distributed Hydrological Model mHM using Satellite derived Land Surface Temperature

    Science.gov (United States)

    Zink, M.; Samaniego, L. E.; Cuntz, M.

    2012-12-01

    A combined investigation of the water and energy balance in hydrologic models can lead to a more accurate estimation of hydrological fluxes and state variables, such as evapotranspiration and soil moisture. Hydrologic models are usually calibrated against discharge measurements, and thus are only trained on information of few points within a catchment. This procedure does not take into account any spatio-temporal variability of fluxes or state variables. Satellite data are a useful source of information to account for this spatial distributions. The objective of this study is to calibrate the distributed hydrological model mHM with satellite derived Land Surface Temperature (LST) fields provided by the Land Surface Analysis - Satellite Application Facility (LSA-SAF). LST is preferred to other satellite products such as soil moisture or evapotranspiration due to its higher precision. LST is obtained by solving the energy balance by assuming that the soil heat flux and the storage term are negligible on a daily time step. The evapotranspiration is determined by closing the water balance in mHM. The net radiation is calculated by using the incoming short- and longwave radiation, albedo and emissivity data provided by LSA-SAF. The Multiscale Parameter Regionalization technique (MPR, Samaniego et al. 2010) is used to determine the aerodynamic resistance among other parameters. The optimization is performed within the time period 2008-2010 using three objective functions that consider 1) only discharge, 2) only LST, and 3) a combination of both. The proposed method is applied to seven major German river basins: Danube, Ems, Main, Mulde, Neckar, Saale, and Weser. The annual coefficient of correlation between LSA-SAF incoming shortwave radiation and 28 meteorological stations operated by the German Weather Service (DWD) is 0.94 (RMSE = 29 W m-2) in 2009. LSA-SAF incoming longwave radiation could be further evaluated at two eddy covariance stations with a very similar

  8. Comparison and evaluation of satellite derived precipitation products for hydrological modeling of the Zambezi River Basin

    Directory of Open Access Journals (Sweden)

    T. Cohen Liechti

    2011-08-01

    Full Text Available In the framework of the African Dams ProjecT (ADAPT, an integrated water resource management study in the Zambezi Basin is currently under development. In view of the sparse gauging network for rainfall monitoring, the observations from spaceborne instrumentation currently produce the only available rainfall data for a large part of the basin.

    Three operational and acknowledged high resolution satellite derived estimates: the Tropical Rainfall Measuring Mission product 3B42 (TRMM 3B42, the Famine Early Warning System product 2.0 (FEWS RFE2.0 and the National Oceanic and Atmospheric Administration/Climate Prediction Centre (NOAA/CPC morphing technique (CMORPH are analyzed in terms of spatial and temporal repartition of the precipitations. They are compared to ground data for the wet seasons of the years 2003 to 2009 on a point to pixel basis at daily, 10-daily and monthly time steps and on a pixel to pixel basis for the wet seasons of the years 2003 to 2007 at monthly time steps.

    The general North-South gradient of precipitation is captured by all the analyzed products. Regarding the spatial heterogeneity, FEWS pixels are much more inter-correlated than TRMM and CMORPH pixels. For a rainfall homogeneity threshold criterion of 0.5 global mean correlation coefficient, the area of each subbasin should not exceed a circle of 2.5° latitude/longitude radius for FEWS and a circle of 0.75° latitude/longitude radius for TRMM and CMORPH considering rectangular mesh.

    In terms of reliability, the correspondence of all estimates with ground data increases with the time step chosen for the analysis. The volume ratio computation indicates that CMORPH is overestimating by nearly 1.5 times the rainfall. The statistics of TRMM and FEWS estimates show quite similar results.

    Due to the its lower inter-correlation and longer data set, the TRMM 3B42 product is chosen as input for the hydraulic-hydrologic model of the basin.

  9. Detecting shifts in tropical moisture imbalances with satellite-derived isotope ratios in water vapor

    Science.gov (United States)

    Bailey, A.; Blossey, P. N.; Noone, D.; Nusbaumer, J.; Wood, R.

    2017-06-01

    As global temperatures rise, regional differences in evaporation (E) and precipitation (P) are likely to become more disparate, causing the drier E-dominated regions of the tropics to become drier and the wetter P-dominated regions to become wetter. Models suggest that such intensification of the water cycle should already be taking place; however, quantitatively verifying these changes is complicated by inherent difficulties in measuring E and P with sufficient spatial coverage and resolution. This paper presents a new metric for tracking changes in regional moisture imbalances (e.g., E-P) by defining δDq—the isotope ratio normalized to a reference water vapor concentration of 4 mmol mol-1—and evaluates its efficacy using both remote sensing retrievals and climate model simulations in the tropics. By normalizing the isotope ratio with respect to water vapor concentration, δDq isolates the portion of isotopic variability most closely associated with shifts between E- and P-dominated regimes. Composite differences in δDq between cold and warm phases of El Niño-Southern Oscillation (ENSO) verify that δDq effectively tracks changes in the hydrological cycle when large-scale convective reorganization takes place. Simulated δDq also demonstrates sensitivity to shorter-term variability in E-P at most tropical locations. Since the isotopic signal of E-P in free tropospheric water vapor transfers to the isotope ratios of precipitation, multidecadal observations of both water vapor and precipitation isotope ratios should provide key evidence of changes in regional moisture imbalances now and in the future.

  10. A study of structural differences between liver cancer cells and normal liver cells using FTIR spectroscopy

    Science.gov (United States)

    Sheng, Daping; Xu, Fangcheng; Yu, Qiang; Fang, Tingting; Xia, Junjun; Li, Seruo; Wang, Xin

    2015-11-01

    Since liver cancer seriously threatens human health, it is very urgent to explore an effective method for diagnosing liver cancer early. In this study, we investigated the structure differences of IR spectra between neoplastic liver cells and normal liver cells. The major differences of absorption bands were observed between liver cancer cells and normal liver cells, the values of A2955/A2921, A1744/A1082, A1640/A1535, H1121/H1020 might be potentially useful factors for distinguishing liver cancer cells from normal liver cells. Curve fitting also provided some important information on structural differences between malignant and normal liver cancer cells. Furthermore, IR spectra combined with hierarchical cluster analysis could make a distinction between liver cancer cells and normal liver cells. The present results provided enough cell basis for diagnosis of liver cancer by FTIR spectroscopy, suggesting FTIR spectroscopy may be a potentially useful tool for liver cancer diagnosis.

  11. Food Consumption Patterns in Mediterranean Adolescents: Are There Differences between Overweight and Normal-Weight Adolescents?

    Science.gov (United States)

    Yannakoulia, Mary; Brussee, Sandra E.; Drichoutis, Andreas C.; Kalea, Anastasia Z.; Yiannakouris, Nikolaos; Matalas, Antonia-Leda; Klimis-Zacas, Dorothy

    2012-01-01

    Objective: To quantify food consumption (based on food group classification) during several time periods in a sample of adolescents and to identify potential differences in food patterns between normal-weight and overweight participants. Design: Cross-sectional study. Participants were classified as normal weight and overweight/obese. Dietary…

  12. Food Consumption Patterns in Mediterranean Adolescents: Are There Differences between Overweight and Normal-Weight Adolescents?

    Science.gov (United States)

    Yannakoulia, Mary; Brussee, Sandra E.; Drichoutis, Andreas C.; Kalea, Anastasia Z.; Yiannakouris, Nikolaos; Matalas, Antonia-Leda; Klimis-Zacas, Dorothy

    2012-01-01

    Objective: To quantify food consumption (based on food group classification) during several time periods in a sample of adolescents and to identify potential differences in food patterns between normal-weight and overweight participants. Design: Cross-sectional study. Participants were classified as normal weight and overweight/obese. Dietary…

  13. Analysis of Satellite-Derived Arctic Tropospheric BrO Columns in Conjunction with Aircraft Measurements During ARCTAS and ARCPAC

    Science.gov (United States)

    Choi, S.; Wang, Y.; Salawitch, R. J.; Canty, T.; Joiner, J.; Zeng, T.; Kurosu, T. P.; Chance, K.; Richter, A.; Huey, L. G.; hide

    2012-01-01

    We derive tropospheric column BrO during the ARCTAS and ARCPAC field campaigns in spring 2008 using retrievals of total column BrO from the satellite UV nadir sensors OMI and GOME-2 using a radiative transfer model and stratospheric column BrO from a photochemical simulation. We conduct a comprehensive comparison of satellite-derived tropospheric BrO column to aircraft in-situ observations ofBrO and related species. The aircraft profiles reveal that tropospheric BrO, when present during April 2008, was distributed over a broad range of altitudes rather than being confined to the planetary boundary layer (PBL). Perturbations to the total column resulting from tropospheric BrO are the same magnitude as perturbations due to longitudinal variations in the stratospheric component, so proper accounting of the stratospheric signal is essential for accurate determination of satellite-derived tropospheric BrO. We find reasonably good agreement between satellite-derived tropospheric BrO and columns found using aircraft in-situ BrO profiles, particularly when satellite radiances were obtained over bright surfaces (albedo> 0.7), for solar zenith angle BrO due to surface processes (the bromine explosion) is apparent in both the OMI and GOME-2 based tropospheric columns. The wide orbital swath of OMI allows examination of the evolution of tropospheric BrO on about hourly time intervals near the pole. Low surface pressure, strong wind, and high PBL height are associated with an observed BrO activation event, supporting the notion of bromine activation by high winds over snow.

  14. Analysis of satellite-derived Arctic tropospheric BrO columns in conjunction with aircraft measurements during ARCTAS and ARCPAC

    Directory of Open Access Journals (Sweden)

    S. Choi

    2012-02-01

    Full Text Available We derive tropospheric column BrO during the ARCTAS and ARCPAC field campaigns in spring 2008 using retrievals of total column BrO from the satellite UV nadir sensors OMI and GOME-2 using a radiative transfer model and stratospheric column BrO from a photochemical simulation. We conduct a comprehensive comparison of satellite-derived tropospheric BrO column to aircraft in-situ observations of BrO and related species. The aircraft profiles reveal that tropospheric BrO, when present during April 2008, was distributed over a broad range of altitudes rather than being confined to the planetary boundary layer (PBL. Perturbations to the total column resulting from tropospheric BrO are the same magnitude as perturbations due to longitudinal variations in the stratospheric component, so proper accounting of the stratospheric signal is essential for accurate determination of satellite-derived tropospheric BrO. We find reasonably good agreement between satellite-derived tropospheric BrO and columns found using aircraft in-situ BrO profiles, particularly when satellite radiances were obtained over bright surfaces (albedo >0.7, for solar zenith angle <80° and clear sky conditions. The rapid activation of BrO due to surface processes (the bromine explosion is apparent in both the OMI and GOME-2 based tropospheric columns. The wide orbital swath of OMI allows examination of the evolution of tropospheric BrO on about hourly time intervals near the pole. Low surface pressure, strong wind, and high PBL height are associated with an observed BrO activation event, supporting the notion of bromine activation by high winds over snow.

  15. NOAA Climate Data Record (CDR) of Normalized Difference Vegetation Index (NDVI), Version 4

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains gridded daily Normalized Difference Vegetation Index (NDVI) derived from the NOAA Climate Data Record (CDR) of Advanced Very High Resolution...

  16. The relationship between satellite-derived indices and species diversity across African savanna ecosystems

    Science.gov (United States)

    Mapfumo, Ratidzo B.; Murwira, Amon; Masocha, Mhosisi; Andriani, R.

    2016-10-01

    The ability to use remotely sensed diversity is important for the management of ecosystems at large spatial extents. However, to achieve this, there is still need to develop robust methods and approaches that enable large-scale mapping of species diversity. In this study, we tested the relationship between species diversity measured in situ with the Normalized Difference Vegetation Index (NDVI) and the Coefficient of Variation in the NDVI (CVNDVI) derived from high and medium spatial resolution satellite data at dry, wet and coastal savanna woodlands. We further tested the effect of logging on NDVI along the transects and between transects as disturbance may be a mechanism driving the patterns observed. Overall, the results of this study suggest that high tree species diversity is associated with low and high NDVI and at intermediate levels is associated with low tree species diversity and NDVI. High tree species diversity is associated with high CVNDVI and vice versa and at intermediate levels is associated with high tree species diversity and CVNDVI.

  17. Spatiotemporal changes of normalized difference vegetation index (NDVI) and response to climate extremes and ecological restoration in the Loess Plateau, China

    Science.gov (United States)

    Zhao, Anzhou; Zhang, Anbing; Liu, Xianfeng; Cao, Sen

    2017-03-01

    Extreme drought, precipitation, and other extreme climatic events often have impacts on vegetation. Based on meteorological data from 52 stations in the Loess Plateau (LP) and a satellite-derived normalized difference vegetation index (NDVI) from the third-generation Global Inventory Modeling and Mapping Studies (GIMMS3g) dataset, this study investigated the relationship between vegetation change and climatic extremes from 1982 to 2013. Our results showed that the vegetation coverage increased significantly, with a linear rate of 0.025/10a (P < 0.001) from 1982 to 2013. As for the spatial distribution, NDVI revealed an increasing trend from the northwest to the southeast, with about 61.79% of the LP exhibiting a significant increasing trend (P < 0.05). Some temperature extreme indices, including TMAXmean, TMINmean, TN90p, TNx, TX90p, and TXx, increased significantly at rates of 0.77 mm/10a, 0.52 °C/10a, 0.62 °C/10a, 0.80 °C/10a, 5.16 days/10a, and 0.65 °C/10a, respectively. On the other hand, other extreme temperature indices including TX10p and TN10p decreased significantly at rates of -2.77 days/10a and 4.57 days/10a (P < 0.01), respectively. Correlation analysis showed that only TMINmean had a significant relationship with NDVI at the yearly time scale (P < 0.05). At the monthly time scale, vegetation coverage and different vegetation types responded significantly positively to precipitation and temperature extremes (TMAXmean, TMINmean, TNx, TNn, TXn, and TXx) (P < 0.01). All of the precipitation extremes and temperature extremes exhibited significant positive relationships with NDVI during the spring and autumn (P < 0.01). However, the relationship between NDVI and RX1day, TMAXmean, TXn, and TXx was insignificant in summer. Vegetation exhibited a significant negative relationship with precipitation extremes in winter (P < 0.05). In terms of human activity, our results indicate a strong correlation between the cumulative afforestation area and NDVI in Yan

  18. Normal and retarded children's understanding of semantic relations in different verbal contexts.

    Science.gov (United States)

    Duchan, J F; Erickson, J G

    1976-12-01

    The effect of different semantic relations presented in different verbal contexts to language retarded and normal children at the one-and two-word stage of development was studied. No significant difference was found between the performance of mentally retarded language-disordered and normal children on the verbal comprehension task. Both groups of children performed best on the possessive, next on the agent-object, then actor-action, and poorest on the locative relations. Finally, nonsense, telegraphic, and expanded contexts did make a difference in the children's understandings with expanded being the best, telegraphic next, and nonsense contexts poorest. Theoretical and clinical implications are discussed.

  19. Analysis of satellite-derived Arctic tropospheric BrO columns in conjunction with aircraft measurements during ARCTAS and ARCPAC

    Directory of Open Access Journals (Sweden)

    S. Choi

    2011-09-01

    Full Text Available We derive estimates of tropospheric BrO column amounts during two Arctic field campaigns in 2008 using information from the satellite UV nadir sensors Ozone Monitoring Instrument (OMI and the second Global Ozone Monitoring Experiment (GOME-2 as well as estimates of stratospheric BrO columns from a model simulation. The sensitivity of the satellite-derived tropospheric BrO columns to various parameters is investigated using a radiative transfer model. We conduct a comprehensive analysis of satellite-derived tropospheric BrO columns including a detailed comparison with aircraft in-situ observations of BrO and related species obtained during the field campaigns. In contrast to prior expectation, tropospheric BrO, when present, existed over a broad range of altitudes. Our results show reasonable agreement between tropospheric BrO columns derived from the satellite observations and columns found using aircraft in-situ BrO. After accounting for the stratospheric contribution to total BrO column, several events of rapid BrO activation due to surface processes in the Arctic are apparent in both the OMI and GOME-2 based tropospheric columns. The wide orbital swath of OMI allows examination of the evolution of tropospheric BrO on about hourly time intervals near the pole. Low pressure systems, strong surface winds, and high planetary boundary layer heights are associated with the observed tropospheric BrO activation events.

  20. [Metabolic differences between males and females and between normal and obese subjects during total fast].

    Science.gov (United States)

    Göschke, H; Girard, J; Stahl, M

    1976-06-01

    In 24 normal and 24 obese subjects of both sexes circulating substrates (blood sugar, free fatty acids, ketone bodies) and hormones (insulin, growth hormone, pancreatic glucagon) were determined during 6 days of total fast. In normals the blood sugar fell to lower levels than in the obese. Plasma free fatty acids and ketone concentrations rose faster in normal than in obese subjects, and faster in females than in males. Plasma insulin concentrations declined to a greater extent in obese than in normal subjects. In all groups studied a significant increase of the pancreatic glucagon level within 1-3 days of fasting was observed, however, its rise occurred faster in normal females than in males. Growth hormone (GH) rose significantly in normal males but not in obese males. Following high overnight fasting values in some normal females showed no significant increase in GH levels but significantly higher GH values than obese females after 1-6 days of fasting. After summarizing starvation-induced metabolic changes common to all study groups the respective differences found between males and females and between normal and obese subjects are discussed.

  1. A Satellite-Derived Climatological Analysis of Urban Heat Island over Shanghai during 2000–2013

    Directory of Open Access Journals (Sweden)

    Weijiao Huang

    2017-06-01

    Full Text Available The urban heat island is generally conducted based on ground observations of air temperature and remotely sensing of land surface temperature (LST. Satellite remotely sensed LST has the advantages of global coverage and consistent periodicity, which overcomes the weakness of ground observations related to sparse distributions and costs. For human related studies and urban climatology, canopy layer urban heat island (CUHI based on air temperatures is extremely important. This study has employed remote sensing methodology to produce monthly CUHI climatology maps during the period 2000–2013, revealing the spatiotemporal characteristics of daytime and nighttime CUHI during this period of rapid urbanization in Shanghai. Using stepwise linear regression, daytime and nighttime air temperatures at the four overpass times of Terra/Aqua were estimated based on time series of Terra/Aqua-MODIS LST and other auxiliary variables including enhanced vegetation index, normalized difference water index, solar zenith angle and distance to coast. The validation results indicate that the models produced an accuracy of 1.6–2.6 °C RMSE for the four overpass times of Terra/Aqua. The models based on Terra LST showed higher accuracy than those based on Aqua LST, and nighttime air temperature estimation had higher accuracy than daytime. The seasonal analysis shows daytime CUHI is strongest in summer and weakest in winter, while nighttime CUHI is weakest in summer and strongest in autumn. The annual mean daytime CUHI during 2000–2013 is 1.0 and 2.2 °C for Terra and Aqua overpass, respectively. The annual mean nighttime CUHI is about 1.0 °C for both Terra and Aqua overpass. The resultant CUHI climatology maps provide a spatiotemporal quantification of CUHI with emphasis on temperature gradients. This study has provided information of relevance to urban planners and environmental managers for assessing and monitoring urban thermal environments which are constantly

  2. Timbre discrimination in normal-hearing and hearing-impaired listeners under different noise conditions.

    Science.gov (United States)

    Emiroglu, Suzan; Kollmeier, Birger

    2008-07-18

    In an attempt to quantify differences in object separation and timbre discrimination between normal-hearing and hearing-impaired listeners with a moderate sensorineural hearing loss of two different configurations, psychoacoustic measurements were performed with a total of 50 listeners. The experiments determined just noticeable differences (JND) of timbre in normal-hearing and hearing-impaired subjects along continua of "morphed" musical instruments and investigated the variance of JND in silence and different background noise conditions and on different sound levels. The results show that timbre JNDs of subjects with a steep hearing loss are significantly higher than of normal-hearing subjects, both in silence and noise, whereas timbre JNDs of flat/diagonal hearing-impaired subjects are similar to JNDs of normal-hearing subjects for signal levels above 55 dB (plus appropriate amplification for hearing-impaired). In noise (SNR=+10 dB) timbre JNDs of all subject groups are significantly higher than in silence. In the condition testing, transferability from silence to noise (i.e., the ability to imagine how the stimulus heard in silence would sound in noise), no significant JND differences across listener groups were found. The results can be explained by primary factors involved in sensorineural hearing loss and contradict the hypothesis that hearing-impaired people generally have more problems in object discrimination than normal-hearing people.

  3. Pathway-specific differences between tumor cell lines and normal and tumor tissue cells

    Directory of Open Access Journals (Sweden)

    Tozeren Aydin

    2006-11-01

    Full Text Available Abstract Background Cell lines are used in experimental investigation of cancer but their capacity to represent tumor cells has yet to be quantified. The aim of the study was to identify significant alterations in pathway usage in cell lines in comparison with normal and tumor tissue. Methods This study utilized a pathway-specific enrichment analysis of publicly accessible microarray data and quantified the gene expression differences between cell lines, tumor, and normal tissue cells for six different tissue types. KEGG pathways that are significantly different between cell lines and tumors, cell lines and normal tissues and tumor and normal tissue were identified through enrichment tests on gene lists obtained using Significance Analysis of Microarrays (SAM. Results Cellular pathways that were significantly upregulated in cell lines compared to tumor cells and normal cells of the same tissue type included ATP synthesis, cell communication, cell cycle, oxidative phosphorylation, purine, pyrimidine and pyruvate metabolism, and proteasome. Results on metabolic pathways suggested an increase in the velocity nucleotide metabolism and RNA production. Pathways that were downregulated in cell lines compared to tumor and normal tissue included cell communication, cell adhesion molecules (CAMs, and ECM-receptor interaction. Only a fraction of the significantly altered genes in tumor-to-normal comparison had similar expressions in cancer cell lines and tumor cells. These genes were tissue-specific and were distributed sparsely among multiple pathways. Conclusion Significantly altered genes in tumors compared to normal tissue were largely tissue specific. Among these genes downregulation was a major trend. In contrast, cell lines contained large sets of significantly upregulated genes that were common to multiple tissue types. Pathway upregulation in cell lines was most pronounced over metabolic pathways including cell nucleotide metabolism and oxidative

  4. Comparisons between buoy-observed, satellite-derived, and modeled surface shortwave flux over the subtropical North Atlantic during the Subduction Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Waliser, Duane E. [Institute for Terrestrial and Planetary Atmospheres, State University of New York, Stony Brook (United States); Weller, Robert A. [Woods Hole Oceanographic Institution, Woods Hole, Massachusetts (United States); Cess, Robert D. [Institute for Terrestrial and Planetary Atmospheres, State University of New York, Stony Brook (United States)

    1999-12-27

    satellite-derived climatologies. These comparisons showed much better and more consistent agreement, with relative bias errors ranging from about -1 to 6%. Comparisons to contemporaneous, daily-average satellite derived values show relatively good agreement as well, with relative biases of the order of 2% ({approx}3-9 W m-2) and root-mean-square differences of {approx}10% (25-30 W m-2). Aspects of the role aerosols play in the above results are discussed along with the implications of the above results on the integrity of open-ocean buoy measurements of surface shortwave flux and the possibility of using the techniques developed in this study to remotely monitor the operating condition of buoy-based shortwave radiometers. (c) 1999 American Geophysical Union.

  5. Normalizing computed tomography data reconstructed with different filter kernels: effect on emphysema quantification

    Energy Technology Data Exchange (ETDEWEB)

    Gallardo-Estrella, Leticia; Prokop, Mathias [Radboud University Nijmegen Medical Center, Geert Grooteplein 10 (route 767), P.O. Box 9101, Nijmegen (766) (Netherlands); Lynch, David A.; Stinson, Douglas; Zach, Jordan [National Jewish Health, Denver, CO (United States); Judy, Philip F. [Brigham and Women' s Hospital, Boston, MA (United States); Ginneken, Bram van; Rikxoort, Eva M. van [Radboud University Nijmegen Medical Center, Geert Grooteplein 10 (route 767), P.O. Box 9101, Nijmegen (766) (Netherlands); Fraunhofer MEVIS, Bremen (Germany)

    2016-02-15

    To propose and evaluate a method to reduce variability in emphysema quantification among different computed tomography (CT) reconstructions by normalizing CT data reconstructed with varying kernels. We included 369 subjects from the COPDGene study. For each subject, spirometry and a chest CT reconstructed with two kernels were obtained using two different scanners. Normalization was performed by frequency band decomposition with hierarchical unsharp masking to standardize the energy in each band to a reference value. Emphysema scores (ES), the percentage of lung voxels below -950 HU, were computed before and after normalization. Bland-Altman analysis and correlation between ES and spirometry before and after normalization were compared. Two mixed cohorts, containing data from all scanners and kernels, were created to simulate heterogeneous acquisition parameters. The average difference in ES between kernels decreased for the scans obtained with both scanners after normalization (7.7 ± 2.7 to 0.3 ± 0.7; 7.2 ± 3.8 to -0.1 ± 0.5). Correlation coefficients between ES and FEV{sub 1}, and FEV{sub 1}/FVC increased significantly for the mixed cohorts. Normalization of chest CT data reduces variation in emphysema quantification due to reconstruction filters and improves correlation between ES and spirometry. (orig.)

  6. Surprising Lack of Sex Differences in Normal Cognitive Aging in Twins

    Science.gov (United States)

    Finkel, Deborah; Reynolds, Chandra A.; Berg, Stig; Pedersen, Nancy L.

    2006-01-01

    Sex differences in the etiology of normal cognitive functioning in aging remain largely unexplored. We conducted an investigation of genetic and environmental contributions to sex differences in level of cognitive performance and rate of decline in the Swedish Adoption/Twin Study of Aging (SATSA) (Finkel & Pedersen, 2004) data set. Behavioral…

  7. Evaluation of the ISBA-TRIP continental hydrologic system over the Niger basin using in situ and satellite derived datasets

    Science.gov (United States)

    Pedinotti, V.; Boone, A.; Decharme, B.; Crétaux, J. F.; Mognard, N.; Panthou, G.; Papa, F.; Tanimoun, B. A.

    2012-06-01

    During the 1970s and 1980s, West Africa has faced extreme climate variations with extended drought conditions. Of particular importance is the Niger basin, since it traverses a large part of the Sahel and is thus a critical source of water for an ever-increasing local population in this semi arid region. However, the understanding of the hydrological processes over this basin is currently limited by the lack of spatially distributed surface water and discharge measurements. The purpose of this study is to evaluate the ability of the ISBA-TRIP continental hydrologic system to represent key processes related to the hydrological cycle of the Niger basin. ISBA-TRIP is currently used within a coupled global climate model, so that the scheme must represent the first order processes which are critical for representing the water cycle while retaining a limited number of parameters and a simple representation of the physics. To this end, the scheme uses first-order approximations to account explicitly for the surface river routing, the floodplain dynamics, and the water storage using a deep aquifer reservoir. In the current study, simulations are done at a 0.5 by 0.5° spatial resolution over the 2002-2007 period (in order to take advantage of the recent satellite record and data from the African Monsoon Multidisciplinary Analyses project, AMMA). Four configurations of the model are compared to evaluate the separate impacts of the flooding scheme and the aquifer on the water cycle. Moreover, the model is forced by two different rainfall datasets to consider the sensitivity of the model to rainfall input uncertainties. The model is evaluated using in situ discharge measurements as well as satellite derived flood extent, total continental water storage changes and river height changes. The basic analysis of in situ discharges confirms the impact of the inner delta area, known as a significant flooded area, on the discharge, characterized by a strong reduction of the

  8. Evaluation of the ISBA-TRIP continental hydrologic system over the Niger basin using in situ and satellite derived datasets

    Directory of Open Access Journals (Sweden)

    V. Pedinotti

    2012-06-01

    Full Text Available During the 1970s and 1980s, West Africa has faced extreme climate variations with extended drought conditions. Of particular importance is the Niger basin, since it traverses a large part of the Sahel and is thus a critical source of water for an ever-increasing local population in this semi arid region. However, the understanding of the hydrological processes over this basin is currently limited by the lack of spatially distributed surface water and discharge measurements. The purpose of this study is to evaluate the ability of the ISBA-TRIP continental hydrologic system to represent key processes related to the hydrological cycle of the Niger basin. ISBA-TRIP is currently used within a coupled global climate model, so that the scheme must represent the first order processes which are critical for representing the water cycle while retaining a limited number of parameters and a simple representation of the physics. To this end, the scheme uses first-order approximations to account explicitly for the surface river routing, the floodplain dynamics, and the water storage using a deep aquifer reservoir. In the current study, simulations are done at a 0.5 by 0.5° spatial resolution over the 2002–2007 period (in order to take advantage of the recent satellite record and data from the African Monsoon Multidisciplinary Analyses project, AMMA. Four configurations of the model are compared to evaluate the separate impacts of the flooding scheme and the aquifer on the water cycle. Moreover, the model is forced by two different rainfall datasets to consider the sensitivity of the model to rainfall input uncertainties. The model is evaluated using in situ discharge measurements as well as satellite derived flood extent, total continental water storage changes and river height changes. The basic analysis of in situ discharges confirms the impact of the inner delta area, known as a significant flooded area, on the discharge, characterized by a strong

  9. Difference in blood microcirculation recovery between normal frostbite and high-altitude frostbite

    Directory of Open Access Journals (Sweden)

    Ming-ke JIAO

    2017-02-01

    Full Text Available Objective To determine the difference in blood microcirculation recovery between normal frostbite and high-altitude frostbite during the wound healing. Methods Twenty four male rats were randomly divided into control group (n=8, normal frostbite group (n=8, and high-altitude group (n=8. The normal frostbite group rats were frozen to produce mid-degree frostbite models by controlling the freezing time with liquid nitrogen penetration equipment. The high-altitude frostbite group rats were acclimated to a hypoxic and low-pressure environment for 1 week, and then the high-altitude frostbite models were constructed by the same way with liquid nitrogen penetration apparatus. On days 3, 7, 11, 15, 19, and 23 after modeling, the recovery situation of blood circulation of each group was observed with contrast ultrasonography by injecting SonoVue micro-bubble into rats' tail. Finally, the micro-bubble concentration (MC was calculated to confirm the blood circulation recovery with software Image Pro. Results At different time points, the wound area of the high-altitude frostbite group was bigger than that of the normal frostbite group, and the MC of control group was always about (27±0.2×109/ml. On day 3, 7, 11, 15, 19, and 23, the MC was significantly lower in the high-altitude frostbite group than in the control group and normal frostbite group (P<0.05. The MC of normal frostbite group was significantly lower than that of the control group on day 3, 7, 11, 15 and 19 (P<0.05. In addition, no obvious difference in MC was found between normal group and control group on the 23th day (P<0.05. Conclusion The blood microcirculation recovery after high-altitude frostbite is significantly slower than the normal frostbite. DOI: 10.11855/j.issn.0577-7402.2017.01.13

  10. Difference in Membrane Repair Capacity Between Cancer Cell Lines and a Normal Cell Line.

    Science.gov (United States)

    Frandsen, Stine Krog; McNeil, Anna K; Novak, Ivana; McNeil, Paul L; Gehl, Julie

    2016-08-01

    Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane repair was investigated by disrupting the plasma membrane using laser followed by monitoring fluorescent dye entry over time in seven cancer cell lines, an immortalized cell line, and a normal primary cell line. The kinetics of repair in living cells can be directly recorded using this technique, providing a sensitive index of repair capacity. The normal primary cell line of all tested cell lines exhibited the slowest rate of dye entry after laser disruption and lowest level of dye uptake. Significantly, more rapid dye uptake and a higher total level of dye uptake occurred in six of the seven tested cancer cell lines (p normal cell line (98 % viable cells) was higher than in the three tested cancer cell lines (81-88 % viable cells). These data suggest more effective membrane repair in normal, primary cells and supplement previous explanations why electroporation-based therapies and other therapies permeabilizing the plasma membrane are more effective on malignant cells compared to normal cells in cancer treatment.

  11. Difference in membrane repair capacity between cancer cell lines and a normal cell line

    DEFF Research Database (Denmark)

    Frandsen, Stine Krog; McNeil, Anna K.; Novak, Ivana

    2016-01-01

    Electroporation-based treatments and other therapies that permeabilize the plasma membrane have been shown to be more devastating to malignant cells than to normal cells. In this study, we asked if a difference in repair capacity could explain this observed difference in sensitivity. Membrane...... repair was investigated by disrupting the plasma membrane using laser followed by monitoring fluorescent dye entry over time in seven cancer cell lines, an immortalized cell line, and a normal primary cell line. The kinetics of repair in living cells can be directly recorded using this technique...... cancer cell lines (p cell line (p membrane permeabilization by electroporation. Viability in the primary normal cell line (98 % viable cells) was higher...

  12. The vector projection of normalized interferogram differences and its application in phase-shift estimation

    Science.gov (United States)

    Li, Teng; Hong, Daxiang

    2016-04-01

    From three interferograms, a novel algorithm for extracting phase shifts based on the vector projection of normalized difference maps is presented. In it, subtraction and vector normalization are operated successively to obtain two normalized interferogram differences without the effect of background component. Then, the phase shift can be estimated based on the analysis and calculation of the vector projection. Without any iteration and complex calculation, this algorithm can be implemented for phase-shift range approximately being well distributed from 0 to 2π, when fringe number of interferograms is more than one. It offers a powerful tool for rapid calibration of phase shifts because of its high efficiency and easy implementation. Numerical simulations and experiments are performed to prove its validity.

  13. Oceanic Weather Decision Support for Unmanned Global Hawk Science Missions into Hurricanes with Tailored Satellite Derived Products

    Science.gov (United States)

    Feltz, Wayne; Griffin, Sarah; Velden, Christopher; Zipser, Ed; Cecil, Daniel; Braun, Scott

    2017-04-01

    The purpose of this presentation is to identify in-flight hazards to high-altitude aircraft, namely the Global Hawk. The Global Hawk was used during Septembers 2012-2016 as part of two NASA funded Hurricane Sentinel-3 field campaigns to over-fly hurricanes in the Atlantic Ocean. This talk identifies the cause of severe turbulence experienced over Hurricane Emily (2005) and how a combination of NOAA funded GOES-R algorithm derived cloud top heights/tropical overshooting tops using GOES-13/SEVIRI imager radiances, and lightning information are used to identify areas of potential turbulence for near real-time navigation decision support. Several examples will demonstrate how the Global Hawk pilots remotely received and used real-time satellite derived cloud and lightning detection information to keep the aircraft safely above clouds and avoid regions of potential turbulence.

  14. Principle and geomorphological applicability of summit level and base level technique using Aster Gdem satellite-derived data and the original software Baz

    Directory of Open Access Journals (Sweden)

    Akihisa Motoki

    2015-05-01

    Full Text Available This article presents principle and geomorphological applicability of summit level technique using Aster Gdem satellite-derived topographicdata. Summit level corresponds to thevirtualtopographic surface constituted bylocalhighest points, such as peaks and plateau tops, and reconstitutes palaeo-geomorphology before the drainage erosion. Summit level map is efficient for reconstitution of palaeo-surfaces and detection of active tectonic movement. Base level is thevirtualsurface composed oflocallowest points, as valley bottoms. The difference between summit level and base level is called relief amount. Thesevirtualmapsareconstructed by theoriginalsoftwareBaz. Themacroconcavity index, MCI, is calculated from summit level and relief amount maps. The volume-normalised three-dimensional concavity index, TCI, is calculated from hypsometric diagram. The massifs with high erosive resistance tend to have convex general form and low MCI and TCI. Those with low resistance have concave form and high MCI and TCI. The diagram of TCI vs. MCI permits to distinguish erosive characteristics of massifs according to their constituent rocks. The base level map for ocean bottom detects the basement tectonic uplift which occurred before the formation of the volcanic seamounts.

  15. Xanthohumol induces different cytotoxicity and apoptotic pathways in malignant and normal astrocytes.

    Science.gov (United States)

    Zajc, I; Filipič, M; Lah, T T

    2012-11-01

    Cytotoxicity and the mechanisms of cell death induced by xanthohumol (XN) were compared in normal and cancerous human cells as the differences may be relevant for the potential use of XN in cancer therapy. The cancer cells seemed to be more susceptible to the cytotoxicity of XN than normal cells, but a significant difference was observed only in astrocytic cells. XN induced a higher rate of apoptosis in glioblastoma cells than in normal astrocytes, which was associated with activation of p53 and an elevated Bax/Bcl-2 ratio in glioblastoma cells, indicating an intrinsic caspase-dependent apoptotic pathway. In contrast, a reduced Bax/Bcl-2 ratio was observed in normal human astrocytes. This was also associated with higher expression of the cell cycle inhibitor, p21, in glioblastoma cells than in normal astrocytes. In addition, at a lower, non-cytotoxic concentration, XN partially inhibited the invasiveness of glioblastoma cells. Due to the selective sensitivity of astrocytic cells to XN, this compound should be studied further as a candidate for adjuvant therapy in the treatment of glioma.

  16. Differences in the Interpretation of Discrepant and Nondiscrepant Messages by Normal and Disturbed Children.

    Science.gov (United States)

    Friend, Margaret; Becker, Judith E.

    1986-01-01

    The report examines research on differences in the interpretations of discrepant and nondiscrepant messages by normal and emotionally disturbed children and reports on a study in which tone of voice and verbal content were discrepant in their affective meaning. A review of the research on discrepant messages, their delivery, interpretation, and…

  17. Shear viscosities and normal stress differences of rigid liquid-crystalline polymers

    NARCIS (Netherlands)

    Tao, Y.G.; den Otter, Wouter K.; Briels, Willem J.

    2006-01-01

    Shear viscosities as well as first and second normal stress differences of solutions of rigid spherocylindrical colloids are investigated by Brownian dynamics simulations for aspect ratios L/D in a range from 25 to 60 and scaled volume fractions L/D from 0.5 to 4.5. Shear thinning behavior is

  18. Craniofacial morphological characteristics of Chinese adults with normal occlusion and different skeletal divergence

    NARCIS (Netherlands)

    Xiao, Danna; Gao, Hui; Ren, Yijin

    2011-01-01

    The aim of the present study was to examine the craniofacial morphologic characteristics of different vertical dysplasias in a population of Chinese adults with normal occlusion. Sixty-nine subjects (39 males and 30 females) were selected from 800 healthy students between 18 and 24 years of age. Lat

  19. Sex-related differences in peripheral glucose metabolism in normal subjects.

    Science.gov (United States)

    Paula, F J; Pimenta, W P; Saad, M J; Paccola, G M; Piccinato, C E; Foss, M C

    1990-01-01

    The metabolic response of muscle tissue to glucose ingestion was studied in 10 normal men (M) and women (F) by using the forearm balance technique and indirect calorimetry simultaneously. During the 3 hours after a 75 g--oral glucose load, glucose uptake per unit muscle mass was significantly higher in women than in men, F = 187.3 +/- 26.9 vs M = 116.7 +/- 9.5 mg/100 g forearm muscle (P less than 0.05). A significant difference in muscle glucose fate was also observed since the amount of glucose utilized through a nonoxidative pathway was significantly higher in women, F = 84.5 +/- 2.6% (161.8 +/- 27.3 mg/100 g forearm muscle) vs M = 75.3 +/- 2.2% (87.2 +/- 8.6 mg/100 g forearm muscle) (P less than 0.05), whereas the amount of glucose oxidized in relation to glucose uptake was significantly higher in men, M = 24.7 +/- 2.2% (28.2 +/- 3.2 mg/100 g forearm muscle) vs F = 15.5 +/- 2.6% (27.8 +/- 5.4 mg/100 g forearm muscle) (P less than 0.05). No significant differences in insulin response to glucose ingestion were detected between groups. The women showed greater suppression of serum free fatty acids (FFA) levels in relation to basal levels than men. We conclude that: 1) after ingesting 75 g glucose, normal women showed greater glucose uptake per unit muscle mass than normal men, 2) for 3 hours after the ingestion of 75 g glucose, the predominant tendency toward utilizing glucose by a nonoxidative pathway is more marked in normal women than in normal men, and 3) the higher glucose uptake per unit muscle mass in the female group in the presence of an insulin response not significantly different from that of the male group suggests that muscle insulin sensitivity is greater in normal women.

  20. A study of ICP-CR difference in children with normal anterior overjet and anterior crossbite

    OpenAIRE

    石川, 亜実; 宇賀, 茂; 桑原, 幹夫; 六車, 武史; 高木, さやか; 丹下, 貴司; 藤川, 隆義; 五十嵐, 清治; 溝口, 到; イシカワ, アミ; ウガ, シゲル; クワバラ, ミキオ; ムグルマ, タケシ; タカギ, サヤカ; タンゲ, タカシ

    2001-01-01

    This study evaluated ICP-CR (Intercuspal Position -Centric Relation) difference at bilateral condyles and incisors in growing patients with anteriro crossbite (mean age; 9 years 2 months), and compared with that of subjects with normal anterior overjet (mean age; 9 years 8 months), using an indicator of the mandibular position, Mandibular Positioning Indicator (MPI). With respect to direction of the ICP-CR difference at condyles and incisors, there were no significant defferences between subj...

  1. Differences between middle cerebral artery bifurcations with normal anatomy and those with aneurysms.

    Science.gov (United States)

    Sadatomo, Takashi; Yuki, Kiyoshi; Migita, Keisuke; Imada, Yasutaka; Kuwabara, Masashi; Kurisu, Kaoru

    2013-07-01

    The objectives of this study were to elucidate the normal anatomy of middle cerebral artery (MCA) bifurcations and to analyze the differences in patients with MCA aneurysms. In the present study, 62 patients underwent three-dimensional magnetic resonance angiography, and no intracranial lesions were noted. The widths of M1 and the superior and inferior M2 branches, as well as their respective lateral angles, were measured. These values were used to calculate the daughter artery ratio (DA ratio; width of larger M2/width of smaller M2) and the lateral angle ratio (LA ratio; lateral angle between M1 and larger M2/lateral angle between M1 and smaller M2). The DA and LA ratios of 54 MCA aneurysm patients (34 with ruptured aneurysms, 20 with unruptured aneurysms) were also calculated, using three-dimensional digital subtraction angiography, and compared with the normal values. In normal patients, the widths of M1 and the branches of M2, the lateral angles, and the LA and DA ratios were not significantly different between the right and left sides. The bilateral superior and inferior lateral angles of normal MCAs were significantly wider than those of MCAs with aneurysms. The DA ratio was 1.5 ± 0.4 in normal MCAs and 1.7 ± 0.7 in MCAs with aneurysms; this difference was significant (p bifurcations show close to symmetric structure in the M2 branches and the lateral angles, whereas aneurysmal MCAs do not show this symmetry.

  2. Satellite-derived estimates of forest leaf area index in southwest Western Australia are not tightly coupled to interannual variations in rainfall: implications for groundwater decline in a drying climate.

    Science.gov (United States)

    Smettem, Keith R J; Waring, Richard H; Callow, John N; Wilson, Melissa; Mu, Qiaozhen

    2013-08-01

    There is increasing concern that widespread forest decline could occur in regions of the world where droughts are predicted to increase in frequency and severity as a result of climate change. The average annual leaf area index (LAI) is an indicator of canopy cover and the difference between the annual maximum and minimum LAI is an indicator of annual leaf turnover. In this study, we analyzed satellite-derived estimates of monthly LAI across forested coastal catchments of southwest Western Australia over a 12 year period (2000-2011) that included the driest year on record for the last 60 years. We observed that over the 12 year study period, the spatial pattern of average annual satellite-derived LAI values was linearly related to mean annual rainfall. However, interannual changes to LAI in response to changes in annual rainfall were far less than expected from the long-term LAI-rainfall trend. This buffered response was investigated using a physiological growth model and attributed to availability of deep soil moisture and/or groundwater storage. The maintenance of high LAIs may be linked to a long-term decline in areal average underground water storage and diminished summer flows, with an emerging trend toward more ephemeral flow regimes.

  3. Different Coexpressions of Arthritis-Relevant Genes between Different Body Organs and Different Brain Regions in the Normal Mouse Population

    OpenAIRE

    Cao, Yanhong; Huang, Yue; Wang, Lishi; Zhu, JiaQian; Gu, Weikuan

    2012-01-01

    Structural changes in different parts of the brain in rheumatoid arthritis (RA) patients have been reported. RA is not regarded as a brain disease. Body organs such as spleen and lung produce RA-relevant genes. We hypothesized that the structural changes in the brain are caused by changes of gene expression in body organs. Changes in different parts of the brain may be affected by altered gene expressions in different body organs. This study explored whether an association between gene expres...

  4. Gene expression profile differences in gastric cancer, pericancerous epithelium and normal gastric mucosa by gene chip

    Institute of Scientific and Technical Information of China (English)

    Chuan-Ding Yu; Shen-Hua Xu; Hang-Zhou Mou; Zhi-Ming Jiang; Chi-Hong Zhu; Xiang-Lin Liu

    2005-01-01

    AIM: To study the difference of gene expression in gastric cancer (T), pericancerous epithelium (P) and normal tissue of gastric mucosa (C), and to screen an associated novel gene in early gastric carcinogenesis by oligonudeotide microarray.METHODS: U133A (Affymetrix, Santa Clara, CA) gene chip was used to detect the gene expression profile difference in T, P and C, respectively. Bioinformatics was used to analyze the detected results.RESULTS: When gastric cancer was compared with normal gastric mucosa, 766 genes were found, with a difference of more than four times in expression levels. Of the 766 genes,530 were up-regulated (Signal Log Ratio [SLR]>2), and 236 were down-regulated (SLR<-2). When pericancerous epithelium was compared with normal gastric mucosa, 64genes were found, with a difference of more than four times in expression levels. Of the 64 genes, 50 were up-regulated (SLR>2), and 14 were down-regulated (SLR<-2). Compared with normal gastric mucosa, a total of 143 genes with a difference in expression levels (more than four times, either in cancer or in pericancerous epithelium) were found in gastric cancer (T) and pericancerous epithelium (P). Of the 143 genes, 108 were up-regulated (SLR>2), and 35were down-regulated (SLR<-2).CONCLUSION: To apply a gene chip could find 143 genes associated with the genes of gastric cancer in pericancerous epithelium, although there were no pathological changes in the tissue slices. More interesting, six genes of pericancerous epithelium were up-regulated in comparison with genes of gastric cancer and three genes were down-regulated in comparison with genes of gastric cancer. It is suggested that these genes may be related to the carcinogenesis and development of early gastric cancer.

  5. Origin and quantification of differences between normal and tumor tissues observed by terahertz spectroscopy

    Science.gov (United States)

    Yamaguchi, Sayuri; Fukushi, Yasuko; Kubota, Oichi; Itsuji, Takeaki; Ouchi, Toshihiko; Yamamoto, Seiji

    2016-09-01

    The origin of the differences in the refractive index observed between normal and tumor tissues using terahertz spectroscopy has been described quantitatively. To estimate water content differences in tissues, we prepared fresh and paraffin-embedded samples from rats. An approximately 5% increase of water content in tumor tissues was calculated from terahertz time domain spectroscopy measurements compared to normal tissues. A greater than 15% increase in percentage of cell nuclei per unit area in tumor tissues was observed by hematoxylin and eosin stained samples, which generates a higher refractive index of biological components other than water. Both high water content and high cell density resulted in higher refractive index by approximately 0.05 in tumor tissues. It is predicted that terahertz spectroscopy can also be used to detect brain tumors in human tissue due to the same underlying mechanism as in rats.

  6. Development of gene microarray in screening differently expressed genes in keloid and normal-control skin

    Institute of Scientific and Technical Information of China (English)

    陈伟; 付小兵; 葛世丽; 孙晓庆; 周岗; 赵志力; 盛志勇

    2004-01-01

    Background Keloid is an intricate lesion that is probably regulated by many genes. In this study, the authors used the technique of complementary DNA (cDNA) microarray to analyse abnormal gene expression in keloids and normal control skins. Methods The polymerase chain reaction (PCR) products of 8400 genes were spotted in an array on chemical-material-coated-glass plates. The DNAs were fixed on the glass plates. The total RNAs were isolated from freshly excised human keloid and normal control skins, and the mRNAs were then purified. The mRNA from both keloid and normal control skins were reversely transcribed to cDNAs, with the incorporation of fluorescent dUTP, for preparing the hybridisation probes. The mixed probes were then hybridised to the cDNA microarray. After thorough washing, the cDNA microarray was scanned for differing fluorescent signals from two types of tissues. Gene expression of tissue growth factor-β1 (TGF-β1) and of c-myc was detected with both RT-PCR and Northern blot hybridisation to confirm the effectiveness of cDNA microarray. Results Among the 8400 human genes, 402 were detected with different expression levels between keloid and normal control skins. Two hundred and fifty genes, including TGF-β1 and c-myc, were up-regulated and 152 genes were down-regulated. Higher expressions of TGF-β1 and c-myc in keloid were also revealed using RT-PCR and Northern blot methods. Conclusion cDNA microarray analysis provides a powerful tool for investigating differential gene expression in keloid and normal control skins. Keloid is a complicated lesion with many genes involved.

  7. Evapotranspiration estimation using a normalized difference vegetation index transformation of satellite data

    Science.gov (United States)

    Seevers, P.M.; Ottmann, R. W.

    1994-01-01

    Evapotranspiration of irrigated crops on two irrigation service areas along the lower Colorado River was estimated using a normalized difference vegetation index of satellite data. A procedure was developed which equated the index to crop coefficients. Evapotranspiration estimates for fields for three dates of thematic mapper data were highly correlated with ground estimates. Service area estimates using thematic mapper and Advanced Very High Resolution Radiometer data agreed well with estimates based on US Geological Survey gauging station data.

  8. Using a Normalized Sobolev Norm of the Difference rather than the Difference of Sobolev Norms for Quantitative Surface Comparison

    CERN Document Server

    Perlin, Marc

    2014-01-01

    In this manuscript we present a method for the quantitative comparison of two surfaces, applicable to temporal and/or spatial extent in one or two dimensions. Often surface comparisons are simply overlaid graphs of results from different methodologies that are qualitative at best; it is the purpose of this work to facilitate quantitative evaluation. The surfaces can be analytical, numerical, and/or experimental, and the result returned by the method, termed surface similarity parameter or normalized error, has been normalized so that its value lies between zero and one. When the parameter has a value of zero, the surfaces are in perfect agreement, whereas a value of one is indicative of perfect disagreement. To provide insight regarding the magnitude of the parameter, several canonical cases are presented, followed by results from breaking water wave experimental measurements with numerical simulations, and by a comparison of a prescribed, periodic, square-wave surface profile and the subsequent manufactured ...

  9. Higher northern latitude normalized difference vegetation index and growing season trends from 1982 to 1999

    Science.gov (United States)

    Tucker, C. J.; Slayback, D. A.; Pinzon, J. E.; Los, S. O.; Myneni, R. B.; Taylor, M. G.

    2001-01-01

    Normalized difference vegetation index data from the polar-orbiting National Oceanic and Atmospheric Administration meteorological satellites from 1982 to 1999 show significant variations in photosynthetic activity and growing season length at latitudes above 35 degrees N. Two distinct periods of increasing plant growth are apparent: 1982-1991 and 1992-1999, separated by a reduction from 1991 to 1992 associated with global cooling resulting from the volcanic eruption of Mt. Pinatubo in June 1991. The average May to September normalized difference vegetation index from 45 degrees N to 75 degrees N increased by 9% from 1982 to 1991, decreased by 5% from 1991 to 1992, and increased by 8% from 1992 to 1999. Variations in the normalized difference vegetation index were associated with variations in the start of the growing season of -5.6, +3.9, and -1.7 days respectively, for the three time periods. Our results support surface temperature increases within the same period at higher northern latitudes where temperature limits plant growth.

  10. Validation of satellite derived LHF using coare_3.0 scheme and time series data over north-east Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Muraleedharan, P.M.; Pankajakshan, T.; Sathe, P.V.

    -6538 versión on-line Gayana (Concepc.) v.68 n.2 supl.TIIProc Concepción 2004 Como citar este artículo Gayana 68(2): 420-426, 2004 VALIDATION OF SATELLITE DERIVED LHF USING COARE_3.0 SCHEME AND TIME SERIES DATA OVER NORTH-EAST INDIAN...

  11. Cyclic alternating patterns in normal sleep and insomnia: structure and content differences.

    Science.gov (United States)

    Chouvarda, Ioanna; Mendez, Martin Oswaldo; Rosso, V; Bianchi, Anna M; Parrino, Liborio; Grassi, Andrea; Terzano, Mario Giovanni; Cerutti, Sergio; Maglaveras, Nicos

    2012-09-01

    This work aims to investigate new markers for the quantitative characterization of insomnia, in the context of sleep microstructure, as expressed by cyclic alternating pattern (CAP) sleep. The study group includes 11 subjects with normal sleep and 10 subjects with diagnosed primary insomnia. Differences between normal sleepers and insomniacs are investigated, in terms of dynamics and content of CAP events. The overall rate of CAP and of different phases is considered. The dynamic in the structure and alternation of CAP events is further studied in different scales by use of wavelet analysis, and calculation of energy/entropy features. The content of CAP events is studied in terms of electroencephalography (EEG) complexity analysis for the different types of events. Statistically significant differences are highlighted, both in structure and content. Besides confirming the increase in CAP rate, main findings regarding the microstructure difference in insomnia include: 1) as regards the deep sleep building phases, more irregular activation-deactivation patterns, with bigger deactivation time, i.e., distance between consecutive activation events, and appearing with higher EEG complexity in deactivation, and 2) a bigger duration of desynchronisation phases, with increased EEG complexity and more irregular patterns. This analysis extends previous findings on the relation between CAPrate increase and sleep instability mechanisms, proposing specific features of CAP that seem to play a role in insomnia (as consistently presented via classification analysis). This opens new perspectives for the understanding of the role of CAP in the quantitative characterization of sleep and its disorders.

  12. Validation of three satellite-derived databases of surface solar radiation using measurements performed at 42 stations in Brazil

    Science.gov (United States)

    Thomas, Claire; Wey, Etienne; Blanc, Philippe; Wald, Lucien

    2016-06-01

    The SoDa website (www.soda-pro.com) is populated with numerous solar-related Web services. Among them, three satellite-derived irradiation databases can be manually or automatically accessed to retrieve radiation values within the geographical coverage of the Meteosat Second Generation (MSG) satellite: the two most advanced versions of the HelioClim-3 database (versions 4 and 5, respectively HC3v4 and HC3v5), and the CAMS radiation service. So far, these databases have been validated against measurements of several stations in Europe and North Africa only. As the quality of such databases depends on the geographical regions and the climates, this paper extends this validation campaign and proposes an extensive comparison on Brazil and global irradiation received on a horizontal surface. Eleven stations from the Brazilian Institute of Space Research (INPE) network offer 1 min observations, and thirty-one stations from the Instituto Nacional de Meteorologia (INMET) network offer hourly observations. The satellite-derived estimates have been compared to the corresponding observations on hourly, daily and monthly basis. The bias relative to the mean of the measurements for HC3v5 is mostly comprised between 1 and 3 %, and that for HC3v4 between 2 and 5 %. These are very satisfactory results and they demonstrate that HC3v5, and to a lesser extent HC3v4, may be used in studies of long-term changes in SSI in Brazil. The situation is not so good with CAMS radiation service for which the relative bias is mostly comprised between 5 and 10 %. For hourly irradiation, the relative RMSE ranges from 15 to 33 %. The correlation coefficient is very large for all stations and the three databases, with an average of 0.96. The three databases reproduce well the hour from hour changes in SSI. The errors show a tendency to increase with the viewing angle of the MSG satellite. They are greater in tropical areas where the relative humidity in the atmosphere is important. It is concluded

  13. Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.

    Science.gov (United States)

    Zhu, Xiaoyang; Li, Xueping; Chen, Weixin; Chen, Jianye; Lu, Wangjin; Chen, Lei; Fu, Danwen

    2012-01-01

    Real-time reverse transcription PCR (RT-qPCR) is a preferred method for rapid and accurate quantification of gene expression studies. Appropriate application of RT-qPCR requires accurate normalization though the use of reference genes. As no single reference gene is universally suitable for all experiments, thus reference gene(s) validation under different experimental conditions is crucial for RT-qPCR analysis. To date, only a few studies on reference genes have been done in other plants but none in papaya. In the present work, we selected 21 candidate reference genes, and evaluated their expression stability in 246 papaya fruit samples using three algorithms, geNorm, NormFinder and RefFinder. The samples consisted of 13 sets collected under different experimental conditions, including various tissues, different storage temperatures, different cultivars, developmental stages, postharvest ripening, modified atmosphere packaging, 1-methylcyclopropene (1-MCP) treatment, hot water treatment, biotic stress and hormone treatment. Our results demonstrated that expression stability varied greatly between reference genes and that different suitable reference gene(s) or combination of reference genes for normalization should be validated according to the experimental conditions. In general, the internal reference genes EIF (Eukaryotic initiation factor 4A), TBP1 (TATA binding protein 1) and TBP2 (TATA binding protein 2) genes had a good performance under most experimental conditions, whereas the most widely present used reference genes, ACTIN (Actin 2), 18S rRNA (18S ribosomal RNA) and GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) were not suitable in many experimental conditions. In addition, two commonly used programs, geNorm and Normfinder, were proved sufficient for the validation. This work provides the first systematic analysis for the selection of superior reference genes for accurate transcript normalization in papaya under different experimental conditions.

  14. Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.

    Directory of Open Access Journals (Sweden)

    Xiaoyang Zhu

    Full Text Available Real-time reverse transcription PCR (RT-qPCR is a preferred method for rapid and accurate quantification of gene expression studies. Appropriate application of RT-qPCR requires accurate normalization though the use of reference genes. As no single reference gene is universally suitable for all experiments, thus reference gene(s validation under different experimental conditions is crucial for RT-qPCR analysis. To date, only a few studies on reference genes have been done in other plants but none in papaya. In the present work, we selected 21 candidate reference genes, and evaluated their expression stability in 246 papaya fruit samples using three algorithms, geNorm, NormFinder and RefFinder. The samples consisted of 13 sets collected under different experimental conditions, including various tissues, different storage temperatures, different cultivars, developmental stages, postharvest ripening, modified atmosphere packaging, 1-methylcyclopropene (1-MCP treatment, hot water treatment, biotic stress and hormone treatment. Our results demonstrated that expression stability varied greatly between reference genes and that different suitable reference gene(s or combination of reference genes for normalization should be validated according to the experimental conditions. In general, the internal reference genes EIF (Eukaryotic initiation factor 4A, TBP1 (TATA binding protein 1 and TBP2 (TATA binding protein 2 genes had a good performance under most experimental conditions, whereas the most widely present used reference genes, ACTIN (Actin 2, 18S rRNA (18S ribosomal RNA and GAPDH (Glyceraldehyde-3-phosphate dehydrogenase were not suitable in many experimental conditions. In addition, two commonly used programs, geNorm and Normfinder, were proved sufficient for the validation. This work provides the first systematic analysis for the selection of superior reference genes for accurate transcript normalization in papaya under different experimental

  15. Validation of Satellite Derived Primary Production Models in the Northeast Atlantic

    Science.gov (United States)

    Lobanova, P. V.; Bashmachnikov, I. L.; Brotas, V.

    2016-08-01

    With all the variety of models used for calculation of primary production of phytoplankton (PP) from remote sensing data, a choice of the most realistic one for a particular ocean region remains a non-trivial issue. In this work, we estimate PP in the Northeast Atlantic Ocean (200 - 510 N and 100 - 400 W) from 1998 to 2005 using three frequently used models: VGPM (Vertically Generalized Production Model), PSM (Platt and Sathyendranath Model) and Aph-PP model (Absorption Based Model). The modeled results are then compared with in situ observations of PP. The results show a close similarity in PP patterns obtained by different models, but the absolute modeled values differ substantially. In the Northeast Atlantic, PSM is found reproducing better the observed seasonal and spatial variability of PP as compared to the two other models. However, PSM slightly underestimates the PP values.

  16. Trend shifts in satellite-derived vegetation growth in Central Eurasia, 1982-2013.

    Science.gov (United States)

    Xu, Hao-Jie; Wang, Xin-Ping; Yang, Tai-Bao

    2017-02-01

    Central Eurasian vegetation is critical for the regional ecological security and the global carbon cycle. However, climatic impacts on vegetation growth in Central Eurasia are uncertain. The reason for this uncertainty lies in the fact that the response of vegetation to climate change showed nonlinearity, seasonality and differences among plant functional types. Based on remotely sensed vegetation index and in-situ meteorological data for the years 1982-2013, in conjunction with the latest land cover type product, we analyzed how vegetation growth trend varied across different seasons and evaluated vegetation response to climate variables at regional, biome and pixel scales. We found a persistent increase in the growing season NDVI over Central Eurasia during 1982-1994, whereas this greening trend has stalled since the mid-1990s in response to increased water deficit. The stalled trend in the growing season NDVI was largely attributed by summer and autumn NDVI changes. Enhanced spring vegetation growth after 2002 was caused by rapid spring warming. The response of vegetation to climatic factors varied in different seasons. Precipitation was the main climate driver for the growing season and summer vegetation growth. Changes in temperature and precipitation during winter and spring controlled the spring vegetation growth. Autumn vegetation growth was mainly dependent on the vegetation growth in summer. We found diverse responses of different vegetation types to climate drivers in Central Eurasia. Forests were more responsive to temperature than to precipitation. Grassland and desert vegetation responded more strongly to precipitation than to temperature in summer but more strongly to temperature than to precipitation in spring. In addition, the growth of desert vegetation was more dependent on winter precipitation than that of grasslands. This study has important implications for improving the performance of terrestrial ecosystem models to predict future vegetation

  17. Ecosystem evaluation (1989-2012) of Ramsar wetland Deepor Beel using satellite-derived indices.

    Science.gov (United States)

    Mozumder, Chitrini; Tripathi, N K; Tipdecho, Taravudh

    2014-11-01

    The unprecedented urban growth especially in developing countries has laid immense pressure on wetlands, finally threatening their existence altogether. A long-term monitoring of wetland ecosystems is the basis of planning conservation measures for a sustainable development. Deepor Beel, a Ramsar wetland and major storm water basin of the River Brahmaputra in the northeastern region of India, needs particular attention due to its constant degradation over the past decades. A rule-based classification algorithm was developed using Landsat (2011)-derived indices, namely Normalised Difference Water Index (NDWI), Modified Normalised Difference Water Index (MNDWI), Normalised Difference Pond Index (NDPI), Normalised Difference Vegetation Index (NDVI) and field data as ancillary information. Field data, ALOS AVNIR and Google Earth images were used for accuracy assessment. A fuzzy accuracy assessment of the classified data sets showed an overall accuracy of 82 % for MAX criteria and 90 % for RIGHT criteria. The rules were used to classify major wetland cover types during low water season (January) in 1989, 2001 and 2012. The statistical analysis of the classified wetland showed heavy manifestation in aquatic vegetation and other features indicating severe eutrophication over the past 23 years. This degradation was closely related to major contributing anthropogenic factors, such as a railway line construction, growing croplands, waste disposal and illegal human settlements in the wetland catchment. In addition, the landscape development index (LDI) indicated a rapid increase in the impact of the surrounding land use on the wetland from 1989 to 2012. The techniques and results from this study may prove useful for top-down landscape analyses of this and other freshwater wetlands.

  18. Developing a new global network of river reaches from merged satellite-derived datasets

    Science.gov (United States)

    Lion, C.; Allen, G. H.; Beighley, E.; Pavelsky, T.

    2015-12-01

    In 2020, the Surface Water and Ocean Topography satellite (SWOT), a joint mission of NASA/CNES/CSA/UK will be launched. One of its major products will be the measurements of continental water extent, including the width, height, and slope of rivers and the surface area and elevations of lakes. The mission will improve the monitoring of continental water and also our understanding of the interactions between different hydrologic reservoirs. For rivers, SWOT measurements of slope must be carried out over predefined river reaches. As such, an a priori dataset for rivers is needed in order to facilitate analysis of the raw SWOT data. The information required to produce this dataset includes measurements of river width, elevation, slope, planform, river network topology, and flow accumulation. To produce this product, we have linked two existing global datasets: the Global River Widths from Landsat (GRWL) database, which contains river centerline locations, widths, and a braiding index derived from Landsat imagery, and a modified version of the HydroSHEDS hydrologically corrected digital elevation product, which contains heights and flow accumulation measurements for streams at 3 arcsecond spatial resolution. Merging these two datasets requires considerable care. The difficulties, among others, lie in the difference of resolution: 30m versus 3 arseconds, and the age of the datasets: 2000 versus ~2010 (some rivers have moved, the braided sections are different). As such, we have developed custom software to merge the two datasets, taking into account the spatial proximity of river channels in the two datasets and ensuring that flow accumulation in the final dataset always increases downstream. Here, we present our preliminary results for a portion of South America and demonstrate the strengths and weaknesses of the method.

  19. Spatial heterogeneity of satellite derived land surface parameters and energy flux densities for LITFASS-area

    Directory of Open Access Journals (Sweden)

    A. Tittebrand

    2009-03-01

    Full Text Available Based on satellite data in different temporal and spatial resolution, the current use of frequency distribution functions (PDF for surface parameters and energy fluxes is one of the most promising ways to describe subgrid heterogeneity of a landscape. Objective of this study is to find typical distribution patterns of parameters (albedo, NDVI for the determination of the actual latent heat flux (L.E determined from highly resolved satellite data within pixel on coarser scale.

    Landsat ETM+, Terra MODIS and NOAA-AVHRR surface temperature and spectral reflectance were used to infer further surface parameters and radiant- and energy flux densities for LITFASS-area, a 20×20 km2 heterogeneous area in Eastern Germany, mainly characterised by the land use types forest, crop, grass and water. Based on the Penman-Monteith-approach L.E, as key quantity of the hydrological cycle, is determined for each sensor in the accordant spatial resolution with an improved parametrisation. However, using three sensors, significant discrepancies between the inferred parameters can cause flux distinctions resultant from differences of the sensor filter response functions or atmospheric correction methods. The approximation of MODIS- and AVHRR- derived surface parameters to the reference parameters of ETM (via regression lines and histogram stretching, respectively, further the use of accurate land use classifications (CORINE and a new Landsat-classification, and a consistent parametrisation for the three sensors were realized to obtain a uniform base for investigations of the spatial variability.

    The analyses for 4 scenes in 2002 and 2003 showed that for forest clear distribution-patterns for NDVI and albedo are found. Grass and crop distributions show higher variability and differ significantly to each other in NDVI but only marginal in albedo. Regarding NDVI-distribution functions NDVI was found to be the key variable for L.E-determination.

  20. MRI investigation of normal fetal lung maturation using signal intensities on different imaging sequences.

    Science.gov (United States)

    Balassy, Csilla; Kasprian, Gregor; Brugger, Peter C; Weber, Michael; Csapo, Bence; Mittermayer, Christoph; Hörmann, Marcus; Prayer, Daniela

    2007-03-01

    To purpose of this paper is to study the relation between normal lung maturation signal and changes in intensity ratios (SIR) and to determine which magnetic resonance imaging sequence provides the strongest correlation of normal lung SIs with gestational age. 126 normal singleton pregnancies (20-37 weeks) were examined with a 1.5 Tesla unit. Mean SIs for lungs, liver, and gastric fluid were assessed on six different sequences, and SIRs of lung/liver (LLSIR) and lung/gastric fluid (LGSIR) were correlated with gestational age for each sequence. To evaluate the feasibility of SIRs in the prediction of the state of the lung maturity, accuracy of the predicted SIRs (D*) was measured by calculating relative residuals (D*-D)/D for each sequence. LLSIRs showed significant changes in every sequence (p<0.05), while LGSIRs only on two sequences. Significant differences were shown for the mean of absolute residuals for both LLSIRs (p<0.001) and for LGSIRs (p=0.003). Relative residuals of LLSIRs were significantly smaller on T1-weighted sequence, whereas they were significantly higher for LGSIRs on FLAIR sequence. Fetal liver seems to be adequate reference for the investigation of lung maturation. T1-weighted sequence was the most accurate for the measurement of the lung SIs; thus, we propose to determine LLSIR on T1-weighted sequence when evaluating lung development.

  1. MRI investigation of normal fetal lung maturation using signal intensities on different imaging sequences

    Energy Technology Data Exchange (ETDEWEB)

    Balassy, Csilla; Kasprian, Gregor; Weber, Michael; Hoermann, Marcus; Prayer, Daniela [Medical University of Vienna, Department of Radiology, Vienna (Austria); Brugger, Peter C. [Medical University of Vienna, Center of Anatomy and Cell Biology, Vienna (Austria); Csapo, Bence [Medical University of Vienna, Department of Obstetrics and Gyneocology, Vienna (Austria); Mittermayer, Christoph [Medical University of Vienna, Department of Pediatrics, Vienna (Austria)

    2007-03-15

    To purpose of this paper is to study the relation between normal lung maturation signal and changes in intensity ratios (SIR) and to determine which magnetic resonance imaging sequence provides the strongest correlation of normal lung SIs with gestational age. 126 normal singleton pregnancies (20-37 weeks) were examined with a 1.5 Tesla unit. Mean SIs for lungs, liver, and gastric fluid were assessed on six different sequences, and SIRs of lung/liver (LLSIR) and lung/gastric fluid (LGSIR) were correlated with gestational age for each sequence. To evaluate the feasibility of SIRs in the prediction of the state of the lung maturity, accuracy of the predicted SIRs (D*) was measured by calculating relative residuals (D*-D)/D for each sequence. LLSIRs showed significant changes in every sequence (p<0.05), while LGSIRs only on two sequences. Significant differences were shown for the mean of absolute residuals for both LLSIRs (p<0.001) and for LGSIRs (p=0.003). Relative residuals of LLSIRs were significantly smaller on T1-weighted sequence, whereas they were significantly higher for LGSIRs on FLAIR sequence. Fetal liver seems to be adequate reference for the investigation of lung maturation. T1-weighted sequence was the most accurate for the measurement of the lung SIs; thus, we propose to determine LLSIR on T1-weighted sequence when evaluating lung development. (orig.)

  2. Differences in expression of retinal proteins between diabetic and normal rats

    Institute of Scientific and Technical Information of China (English)

    Shang-Qing Liu; Jian Kang; Cheng-Jun Li; En-Jie Tang; Bin Wen; Rong Cai; Hui-Jun Yang

    2007-01-01

    AIM: To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes.METHODS: Changes in protein expression of retinal tissues from diabetic and normal rats were observed using 2-dimensional polyacrylamide gel electrophoresis (2-DE). Some protein spots exhibiting statistically significant variations (P < 0.05) were selected randomly and identified by tandem mass spectrometry and analyzed by bioinformatics.RESULTS: 2-DE showed that the expression was upregulated in 5 retinal proteins, down-regulated in 23retinal proteins, and disappeared in 8 retinal proteins.Eight spots were identified from the 36 spots by tandem mass spectrometry (MS/MS) and analyzed by bioinformatics. Guanylate kinase 1, triosephosphate isomerase 1, ATP synthase subunit d, albumin and dimethylarginine dimethylaminohydrolase 2 played an important role in signal transduction. Triosephosphate isomerase 1, crystallin alpha B, ATP synthase subunit d and peroxiredoxin 6 were involved in energy metabolism of retinal tissues. Guanylate kinase 1 played an important role in photoexcitation of retinal rod photoreceptor cells.Whether crystallin beta A1 plays a role in diabetic retinas is unknown so far.CONCLUSION: There are differences in expression of retinal proteins between diabetic and normal rats.These proteins may be involved in the mechanisms and prognosis of retinal diseases caused by diabetes.

  3. Radiometric Normalization of Large Airborne Image Data Sets Acquired by Different Sensor Types

    Science.gov (United States)

    Gehrke, S.; Beshah, B. T.

    2016-06-01

    Generating seamless mosaics of aerial images is a particularly challenging task when the mosaic comprises a large number of im-ages, collected over longer periods of time and with different sensors under varying imaging conditions. Such large mosaics typically consist of very heterogeneous image data, both spatially (different terrain types and atmosphere) and temporally (unstable atmo-spheric properties and even changes in land coverage). We present a new radiometric normalization or, respectively, radiometric aerial triangulation approach that takes advantage of our knowledge about each sensor's properties. The current implementation supports medium and large format airborne imaging sensors of the Leica Geosystems family, namely the ADS line-scanner as well as DMC and RCD frame sensors. A hierarchical modelling - with parameters for the overall mosaic, the sensor type, different flight sessions, strips and individual images - allows for adaptation to each sensor's geometric and radiometric properties. Additional parameters at different hierarchy levels can compensate radiome-tric differences of various origins to compensate for shortcomings of the preceding radiometric sensor calibration as well as BRDF and atmospheric corrections. The final, relative normalization is based on radiometric tie points in overlapping images, absolute radiometric control points and image statistics. It is computed in a global least squares adjustment for the entire mosaic by altering each image's histogram using a location-dependent mathematical model. This model involves contrast and brightness corrections at radiometric fix points with bilinear interpolation for corrections in-between. The distribution of the radiometry fixes is adaptive to each image and generally increases with image size, hence enabling optimal local adaptation even for very long image strips as typi-cally captured by a line-scanner sensor. The normalization approach is implemented in HxMap software. It has been

  4. Multi-site assimilation of a terrestrial biosphere model (BETHY) using satellite derived soil moisture data

    Science.gov (United States)

    Wu, Mousong; Sholze, Marko

    2017-04-01

    We investigated the importance of soil moisture data on assimilation of a terrestrial biosphere model (BETHY) for a long time period from 2010 to 2015. Totally, 101 parameters related to carbon turnover, soil respiration, as well as soil texture were selected for optimization within a carbon cycle data assimilation system (CCDAS). Soil moisture data from Soil Moisture and Ocean Salinity (SMOS) product was derived for 10 sites representing different plant function types (PFTs) as well as different climate zones. Uncertainty of SMOS soil moisture data was also estimated using triple collocation analysis (TCA) method by comparing with ASCAT dataset and BETHY forward simulation results. Assimilation of soil moisture to the system improved soil moisture as well as net primary productivity(NPP) and net ecosystem productivity (NEP) when compared with soil moisture derived from in-situ measurements and fluxnet datasets. Parameter uncertainties were largely reduced relatively to prior values. Using SMOS soil moisture data for assimilation of a terrestrial biosphere model proved to be an efficient approach in reducing uncertainty in ecosystem fluxes simulation. It could be further used in regional an global assimilation work to constrain carbon dioxide concentration simulation by combining with other sources of measurements.

  5. AFM method to detect differences in adhesion of silica bids to cancer and normal epithelial cells

    Science.gov (United States)

    Sokolov, Igor; Iyer, Swaminathan; Gaikwad, Ravi; Woodworth, Craig

    2009-03-01

    To date, the methods of detection of cancer cells have been mostly based on traditional techniques used in biology, such as visual identification of malignant changes, cell growth analysis, specific ligand-receptor labeling, or genetic tests. Despite being well developed, these methods are either insufficiently accurate or require a lengthy complicated analysis. A search for alternative methods for the detection of cancer cells may be a fruitful approach. Here we describe an AFM study that may result in a new method for detection of cancer cells in vitro. Here we use atomic force microscopy (AFM) to study adhesion of single silica beads to malignant and normal cells cultured from human cervix. We found that adhesion depends on the time of contact, and can be statistically different for malignant and normal cells. Using these data, one could develop an optical method of cancer detection based on adhesion of various silica beads.

  6. Mean Normalized Force Computation for Different Types of Obstacles due to Dam Break Using Statistical Techniques

    Directory of Open Access Journals (Sweden)

    Petrus H.A.J.M van Gelder

    2013-05-01

    Full Text Available The dam-break induced loads and their effects on buildings are of vital importance for assessing the vulnerability of buildings in flood-prone areas. A comprehensive methodology, for risk assessment of buildings subject to flooding, is nevertheless still missing. This research aims to take a step forward by following previous research. To this aim, (1 five statistical procedures including: simple correlation analysis, multiple linear regression model, stepwise multiple linear regression model, principal component analysis and cluster analysis are used to study relationship between mean normalized force on structure and other related variables; (2 a new and efficient variable that can take into account both the shape of the structure and flow conditions is proposed; (3 a new and practical formula for predicting the mean normalized force is suggested for different types of obstacles, which is missing in the previous research.

  7. Validation of Satellite-Derived Land Surface Temperature Products - Methods and Good Practice

    Science.gov (United States)

    Guillevic, P. C.; Hulley, G. C.; Hook, S. J.; Biard, J.; Ghent, D.

    2014-12-01

    Land Surface Temperature (LST) is a key variable for surface water and energy budget calculations that can be obtained globally and operationally from satellite observations. LST is used for many applications, including weather forecasting, short-term climate prediction, extreme weather monitoring, and irrigation and water resource management. In order to maximize the usefulness of LST for research and studies it is necessary to know the uncertainty in the LST measurement. Multiple validation methods and activities are necessary to assess LST compliance with the quality specifications of operational users. This work presents four different validation methods that have been widely used to determine the uncertainties in LST products derived from satellite measurements. 1) The temperature based validation method involves comparisons with ground-based measurements of LST. The method is strongly limited by the number and quality of available field stations. 2) Scene-based comparisons involve comparing a new satellite LST product with a heritage LST product. This method is not an absolute validation and satellite LST inter-comparisons alone do not provide an independent validation measurement. 3) The radiance-based validation method does not require ground-based measurements and is usually used for large scale validation effort or for LST products with coarser spatial resolution (> 1km). 4) Time series comparisons are used to detect problems that can occur during the instrument's life, e.g. calibration drift, or unrealistic outliers due to cloud coverage. This study enumerates the sources of errors associated with each method. The four different approaches are complementary and provide different levels of information about the quality of the retrieved LST. The challenges in retrieving the LST from satellite measurements are discussed using results obtained for MODIS and VIIRS. This work contributes to the objective of the Land Product Validation (LPV) sub-group of the

  8. Spatio-temporal variability of satellite derived aerosol optical thickness and ground measurements over East China

    Science.gov (United States)

    Meng, Fei; Shi, Tongguang

    2016-04-01

    Two-year records of Visible Infrared Imaging Radiometer Suite (VIIRS) Intermediate Product (IP) data on the aerosol optical thickness (AOT) at 550 nm were evaluated by comparing them with sun-sky radiometer measurements from the Chinese sun hazemeter network (CSHNET) and the aerosol robotic network (AERONET). The monthly and seasonal variations in the aerosol optical properties over eastern China were then investigated using collocated VIIRS IP data and CSHNET and AERONET measurements.Results show that the performances of the current VIIRS IP AOT retrievals at the provisional stage were consistent with ground measurements. Similar characteristics of seasonal and monthly variations were found among the measurements, though the observational methodologies were different, showing maxima in the summer and spring and minima in the winter and autumn.

  9. Steady state estimation of soil organic carbon using satellite-derived canopy leaf area index

    Science.gov (United States)

    Fang, Yilin; Liu, Chongxuan; Huang, Maoyi; Li, Hongyi; Leung, L. Ruby

    2014-12-01

    Estimation of soil organic carbon (SOC) stock using models typically requires long term spin-up of the carbon-nitrogen (CN) models, which has become a bottleneck for global modeling. We report a new numerical approach to estimate global SOC stock that can alleviate long spin-up. The approach uses satellite-based canopy leaf area index (LAI) and takes advantage of a reaction-based biogeochemical module—Next Generation BioGeoChemical Module (NGBGC) that was recently developed and incorporated in version 4 of the Community Land Model (CLM4). Although NGBGC uses the same CN mechanisms as in CLM4CN, it can be easily configured to run prognostic or steady state simulations. The new approach was applied at point and global scales and compared with SOC derived from spin-up by running NGBGC in the prognostic mode, and SOC from the Harmonized World Soil Database (HWSD). The steady state solution is comparable to the spin-up value when the satellite LAI is close to that from the spin-up solution, and largely captured the global variability of the HWSD SOC across the different dominant plant functional types (PFTs). The correlation between the simulated and HWSD SOC was, however, weak at both point and global scales, suggesting the needs for improving the biogeochemical processes described in CLM4 and updating HWSD. Besides SOC, the steady state solution also includes all other state variables simulated by a spin-up run, which makes the tested approach a promising tool to efficiently estimate global SOC distribution and evaluate and compare multiple aspects simulated by different CN mechanisms in the model.

  10. Comparison of Satellite-Derived Wind Measurements with Other Wind Measurement Sensors

    Science.gov (United States)

    Susko, Michael; Herman, Leroy

    1995-01-01

    The purpose of this paper is to compare the good data from the Jimsphere launches with the data from the satellite system. By comparing the wind speeds from the Fixed Pedestal System 16 (FPS-16) Radar/Jimsphere Wind System and NASA's 50-MHz Radar Wind Profiler, the validation of winds from Geostationary Operational Environmental Satellite 7 (GOES-7) is performed. This study provides an in situ data quality check for the GOES-7 satellite winds. Comparison was made of the flowfields in the troposphere and the lower stratosphere of case studies of pairs of Jimsphere balloon releases and Radar Wind Profiler winds during Space Shuttle launches. The mean and standard deviation of the zonal component statistics, the meridional component statistics, and the power spectral density curves show good agreement between the two wind sensors. The standard deviation of the u and v components for the STS-37 launch (consisting of five Jimsphere/Radar Wind Profiler data sets) was 1.92 and 1.67 m/s, respectively; for the STS-43 launch (there were six Jimsphere/Wind Profiler data sets) it was 1.39 and 1.44 m/s, respectively. The overall standard deviation was 1.66 m/s for the u component and 1.55 m/s tor the v component, and a standard deviation of 2.27 m/s tor the vector wind difference. The global comparison of satellite with Jimsphere balloon vector winds shows a standard deviation of 3.15 m/s for STS-43 and 4.37 m/s for STS-37. The overall standard deviation of the vector wind was 3.76 m/s, with a root-mean-square vector difference of 4.43 m/s. These data have demonstrated that this unique comparison of the Jimsphere and satellite winds provides excellent ground truth and a frame of reference during testing and validation of satellite data

  11. Is the sagittal postural alignment different in normal and dysphonic adult speakers?

    Science.gov (United States)

    Franco, Débora; Martins, Fernando; Andrea, Mário; Fragoso, Isabel; Carrão, Luís; Teles, Júlia

    2014-07-01

    Clinical research in the field of voice disorders, in particular functional dysphonia, has suggested abnormal laryngeal posture due to muscle adaptive changes, although specific evidence regarding body posture has been lacking. The aim of our study was to verify if there were significant differences in sagittal spine alignment between normal (41 subjects) and dysphonic speakers (33 subjects). Cross-sectional study. Seventy-four adults, 35 males and 39 females, were submitted to sagittal plane photographs so that spine alignment could be analyzed through the Digimizer-MedCalc Software Ltd program. Perceptual and acoustic evaluation and nasoendoscopy were used for dysphonic judgments: normal and dysphonic speakers. For thoracic length curvature (TL) and for the kyphosis index (KI), a significant effect of dysphonia was observed with mean TL and KI significantly higher for the dysphonic speakers than for the normal speakers. Concerning the TL variable, a significant effect of sex was found, in which the mean of the TL was higher for males than females. The interaction between dysphonia and sex did not have a significant effect on TL and KI variables. For the lumbar length curvature variable, a significant main effect of sex was demonstrated; there was no significant main effect of dysphonia or significant sex×dysphonia interaction. Findings indicated significant differences in some sagittal spine posture measures between normal and dysphonic speakers. Postural measures can add useful information to voice assessment protocols and should be taken into account when considering particular treatment strategies. Copyright © 2014 The Voice Foundation. Published by Mosby, Inc. All rights reserved.

  12. Time course of auditory streaming: Do CI users differ from normal-hearing listeners?

    Directory of Open Access Journals (Sweden)

    Martin eBöckmann-Barthel

    2014-07-01

    Full Text Available In a complex acoustical environment with multiple sound sources the auditory system uses streaming as a tool to organize the incoming sounds in one or more streams depending on the stimulus parameters. Streaming is commonly studied by alternating sequences of signals. These are often tones with different frequencies. The present study investigates stream segregation in cochlear implant (CI users, where hearing is restored by electrical stimulation of the auditory nerve. CI users listened to 30-s long sequences of alternating A and B harmonic complexes at four different fundamental frequency separations, ranging from 2 to 14 semitones. They had to indicate as promptly as possible after sequence onset, if they perceived one stream or two streams and, in addition, any changes of the percept throughout the rest of the sequence. The conventional view is that the initial percept is always that of a single stream which may after some time change to a percept of two streams. This general build-up hypothesis has recently been challenged on the basis of a new analysis of data of normal-hearing listeners which showed a build-up response only for an intermediate frequency separation. Using the same experimental paradigm and analysis, the present study found that the results of CI users agree with those of the normal-hearing listeners: (i the probability of the first decision to be a one-stream percept decreased and that of a two-stream percept increased as Δf increased, and (ii a build-up was only found for 6 semitones. Only the time elapsed before the listeners made their first decision of the percept was prolonged as compared to normal-hearing listeners. The similarity in the data of the CI user and the normal-hearing listeners indicates that the quality of stream formation is similar in these groups of listeners.

  13. Changes in satellite-derived spring vegetation green-up date and its linkage to climate in China from 1982 to 2010: a multimethod analysis.

    Science.gov (United States)

    Cong, Nan; Wang, Tao; Nan, Huijuan; Ma, Yuecun; Wang, Xuhui; Myneni, Ranga B; Piao, Shilong

    2013-03-01

    The change in spring phenology is recognized to exert a major influence on carbon balance dynamics in temperate ecosystems. Over the past several decades, several studies focused on shifts in spring phenology; however, large uncertainties still exist, and one understudied source could be the method implemented in retrieving satellite-derived spring phenology. To account for this potential uncertainty, we conducted a multimethod investigation to quantify changes in vegetation green-up date from 1982 to 2010 over temperate China, and to characterize climatic controls on spring phenology. Over temperate China, the five methods estimated that the vegetation green-up onset date advanced, on average, at a rate of 1.3 ± 0.6 days per decade (ranging from 0.4 to 1.9 days per decade) over the last 29 years. Moreover, the sign of the trends in vegetation green-up date derived from the five methods were broadly consistent spatially and for different vegetation types, but with large differences in the magnitude of the trend. The large intermethod variance was notably observed in arid and semiarid vegetation types. Our results also showed that change in vegetation green-up date is more closely correlated with temperature than with precipitation. However, the temperature sensitivity of spring vegetation green-up date became higher as precipitation increased, implying that precipitation is an important regulator of the response of vegetation spring phenology to change in temperature. This intricate linkage between spring phenology and precipitation must be taken into account in current phenological models which are mostly driven by temperature. © 2012 Blackwell Publishing Ltd.

  14. Modeling of groundwater draft based on satellite-derived crop acreage estimation over an arid region of northwest India

    Science.gov (United States)

    Bhadra, Bidyut Kumar; Kumar, Sanjay; Paliwal, Rakesh; Jeyaseelan, A. T.

    2016-11-01

    Over-exploitation of groundwater for agricultural crops puts stress on the sustainability of natural resources in the arid region of Rajasthan state, India. Hydrogeological study of groundwater levels of the study area during the pre-monsoon (May to June), post-monsoon (October to November) and post-irrigation (February to March) seasons of 2004-2005 to 2011-2012 shows a steady decline of groundwater levels at the rate of 1.28-1.68 m/year, mainly due to excessive groundwater draft for irrigation. Due to the low density of the groundwater observation-well network in the study area, assessment of groundwater draft, and thus groundwater resource management, becomes a difficult task. To overcome the situation, a linear groundwater draft model (LGDM) has been developed based on the empirical relationship between satellite-derived crop acreage and the observed groundwater draft for the year 2003-2004. The model has been validated for a decade, during three year-long intervals (2005-2006, 2008-2009 and 2011-2012) using groundwater draft, estimated through a discharge factor method. Further, the estimated draft was validated through observed pumping data from random sampled villages (2011-2012). The results suggest that the developed LGDM model provides a good alternative to the estimation of groundwater draft based on satellite-based crop area in the absence of groundwater observation wells in arid regions of northwest India.

  15. Modelling dengue fever risk in the State of Yucatan, Mexico using regional-scale satellite-derived sea surface temperature.

    Science.gov (United States)

    Laureano-Rosario, Abdiel E; Garcia-Rejon, Julian E; Gomez-Carro, Salvador; Farfan-Ale, Jose A; Muller-Karger, Frank E

    2017-08-01

    Accurately predicting vector-borne diseases, such as dengue fever, is essential for communities worldwide. Changes in environmental parameters such as precipitation, air temperature, and humidity are known to influence dengue fever dynamics. Furthermore, previous studies have shown how oceanographic variables, such as El Niño Southern Oscillation (ENSO)-related sea surface temperature from the Pacific Ocean, influences dengue fever in the Americas. However, literature is lacking on the use of regional-scale satellite-derived sea surface temperature (SST) to assess its relationship with dengue fever in coastal areas. Data on confirmed dengue cases, demographics, precipitation, and air temperature were collected. Incidence of weekly dengue cases was examined. Stepwise multiple regression analyses (AIC model selection) were used to assess which environmental variables best explained increased dengue incidence rates. SST, minimum air temperature, precipitation, and humidity substantially explained 42% of the observed variation (r(2)=0.42). Infectious diseases are characterized by the influence of past cases on current cases and results show that previous dengue cases alone explained 89% of the variation. Ordinary least-squares analyses showed a positive trend of 0.20±0.03°C in SST from 2006 to 2015. An important element of this study is to help develop strategic recommendations for public health officials in Mexico by providing a simple early warning capability for dengue incidence. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Modelling LAI at a regional scale with ISBA-A-gs: comparison with satellite-derived LAI over southwestern France

    Directory of Open Access Journals (Sweden)

    A. Brut

    2009-08-01

    Full Text Available A CO2-responsive land surface model (the ISBA-A-gs model of Météo-France is used to simulate photosynthesis and Leaf Area Index (LAI in southwestern France for a 3-year period (2001–2003. A domain of about 170 000 km2 is covered at a spatial resolution of 8 km. The capability of ISBA-A-gs to reproduce the seasonal and the interannual variability of LAI at a regional scale, is assessed with satellite-derived LAI products. One originates from the CYCLOPES programme using SPOT/VEGETATION data, and two products are based on MODIS data. The comparison reveals discrepancies between the satellite LAI estimates and between satellite and simulated LAI values, both in their intensity and in the timing of the leaf onset. The model simulates higher LAI values for the C3 crops than the satellite observations, which may be due to a saturation effect within the satellite signal or to uncertainties in model parameters. The simulated leaf onset presents a significant delay for C3 crops and mountainous grasslands. In-situ observations at a mid-altitude grassland site show that the generic temperature response of photosynthesis used in the model is not appropriate for plants adapted to the cold climatic conditions of the mountainous areas. This study demonstrates the potential of LAI remote sensing products for identifying and locating models' shortcomings at a regional scale.

  17. Modelling LAI at a regional scale with ISBA-A-gs: comparison with satellite-derived LAI over southwestern France

    Directory of Open Access Journals (Sweden)

    A. Brut

    2009-04-01

    Full Text Available A CO2-responsive land surface model (the ISBA-A-gs model of Météo-France is used to simulate photosynthesis and Leaf Area Index (LAI in southwestern France for a 3-year period (2001–2003. A domain of about 170 000 km2 is covered at a spatial resolution of 8 km. The capability of ISBA-A-gs to reproduce the seasonal and the inter-annual variability of LAI at a regional scale, is assessed with two satellite-derived LAI products. One originates from the CYCLOPES programme using SPOT/VEGETATION data, and the second is based on MODIS data. The comparison reveals discrepancies between the two satellite LAI estimates and between satellite and simulated LAI values, both in their intensity and in the timing of the leaf onset. The model simulates higher LAI values for the C3 crops and coniferous trees than the satellite observations, which may be due to a saturation effect within the satellite signal. The simulated leaf onset presents a significant delay for mountainous grasslands. In-situ observations at a mid-altitude grassland site show that the generic temperature response of photosynthesis used in the model is not appropriate for plants adapted to the cold climatic conditions of the mountainous areas. This study demonstrates the potential of LAI remote sensing products for identifying and locating models' shortcomings at a regional scale.

  18. Modelling LAI at a regional scale with ISBA-A-gs: comparison with satellite-derived LAI over southwestern France

    Science.gov (United States)

    Brut, A.; Rüdiger, C.; Lafont, S.; Roujean, J.-L.; Calvet, J.-C.; Jarlan, L.; Gibelin, A.-L.; Albergel, C.; Le Moigne, P.; Soussana, J.-F.; Klumpp, K.

    2009-04-01

    A CO2-responsive land surface model (the ISBA-A-gs model of Météo-France) is used to simulate photosynthesis and Leaf Area Index (LAI) in southwestern France for a 3-year period (2001-2003). A domain of about 170 000 km2 is covered at a spatial resolution of 8 km. The capability of ISBA-A-gs to reproduce the seasonal and the inter-annual variability of LAI at a regional scale, is assessed with two satellite-derived LAI products. One originates from the CYCLOPES programme using SPOT/VEGETATION data, and the second is based on MODIS data. The comparison reveals discrepancies between the two satellite LAI estimates and between satellite and simulated LAI values, both in their intensity and in the timing of the leaf onset. The model simulates higher LAI values for the C3 crops and coniferous trees than the satellite observations, which may be due to a saturation effect within the satellite signal. The simulated leaf onset presents a significant delay for mountainous grasslands. In-situ observations at a mid-altitude grassland site show that the generic temperature response of photosynthesis used in the model is not appropriate for plants adapted to the cold climatic conditions of the mountainous areas. This study demonstrates the potential of LAI remote sensing products for identifying and locating models' shortcomings at a regional scale.

  19. Aerus-GEO: newly available satellite-derived aerosol optical depth product over Europe and Africa

    Science.gov (United States)

    Carrer, D.; Roujean, J. L.; Ceamanos, X.; Six, B.; Suman, S.

    2015-12-01

    The major difficulty in detecting the aerosol signal from visible and near-infrared remote sensing observations is to reach the proper separation of the components related to the atmosphere and the surface. A method is proposed to circumvent this issue by exploiting the directional and temporal dimensions of the satellite signal through the use of a semi-empirical kernel-driven model for the surface/atmosphere coupled system. This algorithm was implemented by the ICARE Data Center (http://www.icare.univ-lille1.fr), which operationally disseminates a daily AOD product at 670 nm over the MSG disk since 2014. The proposed method referred to as AERUS-GEO (Aerosol and surface albEdo Retrieval Using a directional Splitting method - application to GEO data) is applied to three spectral bands (0.6 mm, 0.8 mm, and 1.6 mm) of MSG (Meteosat Second Generation) observations, which scan Europe, Africa, and the Eastern part of South America every 15 minutes. The daily AOD estimates at 0.63μm has been extensively validated. In contrast, the Angstrom coefficient is still going through validation and we will show the differences between the MSG derived Angstrom exponent with that of CAMS (Copernicus Atmosphere Monitoring Service) near-real time aerosol product. The impact of aerosol type on the aerosol radiative forcing will be presented as a part of future development plan.

  20. Comparison between MODIS and AIRS/AMSU satellite-derived surface skin temperatures

    Directory of Open Access Journals (Sweden)

    Y.-R. Lee

    2012-10-01

    Full Text Available Surface skin temperatures of the Version 5 Level 3 products of MODIS and AIRS/AMSU have been compared in terms of monthly anomaly trends and climatology over the globe during the period from September 2002 to August 2011. The MODIS temperatures in the 50° N–50° S region tend to systematically be ~1.7 K colder over land and ~0.5 K warmer over ocean than the AIRS/AMSU temperatures. Over high latitude ocean the MODIS values are ~5.5 K warmer than the AIRS/AMSU. The discrepancies between the annual averages of the two sensors are as much as ~12 K in the sea ice regions. Both MODIS and AIRS/AMSU show cooling trends from −0.05 ± 0.06 to −0.14 ± 0.07 K (9 yr−1 over the globe, but warming trends (0.02 ± 0.12–0.15 ± 0.19 K (9 yr−1 in the high latitude regions. The disagreement between the two sensors results mainly from the differences in ice/snow emissivity between MODIS infrared and AMSU microwave, and also in their observational local times.

  1. Simulation of suspended sediment transport initialized with satellite derived suspended sediment concentrations

    Indian Academy of Sciences (India)

    Ratheesh Ramakrishnan; A S Rajawat

    2012-10-01

    Suspended sediment transport in the Gulf of Kachchh is simulated utilizing the suspended sediment concentration (SSC) derived from Oceansat OCM imagery, as the initial condition in MIKE-21 Mud Transport model. Optimization of the model mud parameters, like settling velocity and critical shear stress for erosion are realized with respect to the sediment size distribution and the bottom bed materials observed in the Gulf. Simulated SSCs are compared with alternate OCM derived SSC. The results are observed to be impetus where the model is able to generate the spatial dynamics of the sediment concentrations. Sediment dynamics like deposition, erosion and dispersion are explained with the simulated tidal currents and OCM derived sediment concentrations. Tidal range is observed as the important physical factor controlling the deposition and resuspension of sediments within the Gulf. From the simulation studies; maximum residual current velocities, tidal fronts and high turbulent zones are found to characterise the islands and shoals within the Gulf, which results in high sediment concentrations in those regions. Remarkable variability in the bathymetry of the Gulf, different bed materials and varying tidal conditions induces several circulation patterns and turbulence creating the unique suspended sediment concentration pattern in the Gulf.

  2. Gene Expression Profile Differences in Gastric Cancer and Normal Gastric Mucosa by Oligonucleotide Microarrays

    Institute of Scientific and Technical Information of China (English)

    Chuanding Yu; Shenhua Xu; HangZhou Mou; Zhiming Jiang; Chihong Zhu; Xianglin Liu

    2006-01-01

    OBJECTIVE To study the difference of gene expression in gastric cancer (T) and normal tissue of gastric mucosa (C), and to screen for associated novel genes in gastric cancers by oligonucleotide microarrays.METHODS U133A (Affymetrix, Santa Clara, CA) gene chip was used to detect the gene expression profile difference in T and C. Bioinformatics was used to analyze the detected results.RESULTS When gastric cancers were compared with normal gastric mucosa, a total of 270 genes were found with a difference of more than 9times in expression levels. Of the 270 genes, 157 were up-regulated (Signal Log Ratio [SLR] ≥3), and 113 were down-regulated (SLR ≤-3).Using a classification of function, the highest number of gene expression differences related to enzymes and their regulatory genes (67, 24.8%),followed by signal-transduction genes (43,15.9%). The third were nucleic acid binding genes (17, 6.3%), fourth were transporter genes (15, 5.5%)and fifth were protein binding genes (12, 4.4%). In addition there were 50genes of unknown function, accounting for 18.5%. The five above mentioned groups made up 56.9% of the total gene number.CONCLUSION The 5 gene groups (enzymes and their regulatory proteins, signal transduction proteins, nucleic acid binding proteins, transporter and protein binding) were abnormally expressed and are important genes for further study in gastric cancers.

  3. Changes in satellite-derived impervious surface area at US historical climatology network stations

    Science.gov (United States)

    Gallo, Kevin; Xian, George

    2016-10-01

    The difference between 30 m gridded impervious surface area (ISA) between 2001 and 2011 was evaluated within 100 and 1000 m radii of the locations of climate stations that comprise the US Historical Climatology Network. The amount of area associated with observed increases in ISA above specific thresholds was documented for the climate stations. Over 32% of the USHCN stations exhibited an increase in ISA of ⩾20% between 2001 and 2011 for at least 1% of the grid cells within a 100 m radius of the station. However, as the required area associated with ISA change was increased from ⩾1% to ⩾10%, the number of stations that were observed with a ⩾20% increase in ISA between 2001 and 2011 decreased to 113 (9% of stations). When the 1000 m radius associated with each station was examined, over 52% (over 600) of the stations exhibited an increase in ISA of ⩾20% within at least 1% of the grid cells within that radius. However, as the required area associated with ISA change was increased to ⩾10% the number of stations that were observed with a ⩾20% increase in ISA between 2001 and 2011 decreased to 35 (less than 3% of the stations). The gridded ISA data provides an opportunity to characterize the environment around climate stations with a consistently measured indicator of a surface feature. Periodic evaluations of changes in the ISA near the USHCN and other networks of stations are recommended to assure the local environment around the stations has not significantly changed such that observations at the stations may be impacted.

  4. Improvements of Satellite-Derived Cyclonic Rainfall over the North Atlantic.

    Science.gov (United States)

    Klepp, Christian-Philipp; Bakan, Stephan; Graßl, Hartmut

    2003-02-01

    Case studies of rainfall, derived from Special Sensor Microwave Imager (SSM/I) satellite data during the passage of individual cyclones over the North Atlantic, are presented to enhance the knowledge of rainfall processes associated with frontal systems. A multisatellite method is applied for complete coverage of the North Atlantic twice a day. Different SSM/I precipitation algorithms have been tested for individual cyclones and compared to the Global Precipitation Climatology Project (GPCP) datasets. An independent rainfall pattern and intensity validation method is presented using voluntary observing ship (VOS) datasets and Advanced Very High Resolution Radiometer (AVHRR) images.Intense cyclones occur frequently in the wintertime period, with cold fronts propagating far south over the North Atlantic. Following upstream, large cloud clusters are frequently embedded in the cellular structured cold air of the backside regions, which produce heavy convective rainfall events, especially in the region off Newfoundland around 50°N. These storms can be easily identified on AVHRR images. It transpired that only the SSM/I rainfall algorithm of Bauer and Schlüssel is sensitive enough to detect the rainfall patterns and intensities observed by VOS for those cyclone types over the North Atlantic. In contrast, the GPCP products do not recognize this backside rainfall, whereas the frontal rainfall conditions are well represented in all tested datasets. This is suggested from the results of an intensive intercomparison study with ship reports from the time period of the Fronts and Atlantic Storm Track Experiment (FASTEX) field campaign. For this purpose, a new technique has been developed to transfer ship report codes into rain-rate estimates. From the analysis of a complete life cycle of a cyclone, it follows that these mesoscale backside rainfall events contribute up to 25% to the total amount of rainfall in North Atlantic cyclones.

  5. Normalizing surface electromyographic measures of the masticatory muscles: Comparison of two different methods for clinical purpose.

    Science.gov (United States)

    Mapelli, Andrea; Tartaglia, Gianluca Martino; Connelly, Stephen Thaddeus; Ferrario, Virgilio Ferruccio; De Felicio, Claudia Maria; Sforza, Chiarella

    2016-10-01

    To compare a new normalization technique (wax pad, WAX) with the currently utilized cotton roll (COT) method in surface electromyography (sEMG) of the masticatory muscles. sEMG of the masseter and anterior temporalis muscles of 23 subjects was recorded while performing two repetitions of 5s maximum voluntary clenches (MVC) on COT and WAX. For each task, the mean value of sEMG amplitude and its coefficient of variation were calculated, and the differences between the two repetitions computed. The standard error of measurement (SEM) was calculated. For each subject and muscle, the COT-to-WAX maximum activity increment was computed. Participant preference between tasks was also recorded. WAX MVC tasks had larger maximum EMG amplitude than COT MVC tasks (P0.391) and its coefficient of variation were unchanged (P>0.180). The WAX task was the more comfortable for 18/23 subjects (P=0.007). WAX normalization ensures the same stability level of maximum EMG amplitude as COT normalization, but it is more repeatable, elicits larger maximum muscular contraction, and is felt to be more comfortable by subjects. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Doses delivered to normal brain under different treatment protocols at Brookhaven National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Capala, J.; Coderre, J.A.; Liu, H.B. [and others

    1996-12-31

    As of October 31, 1996, 23 glioblastoma multiforme patients underwent BNCT under several treatment protocols at the Brookhaven Medical Research Reactor. For treatment planning and dosimetry purposes, these protocols may be divided into four groups. The first group comprises protocols that used an 8-cm collimator and allowed a peak normal brain dose of 10.5 Gy-Eq to avolume of 1 cm{sup 3} were the thermal neutron flux was maximal (even if it happened to be in the tumor volume). The second group differs from the first in that it allowed a peak normal brain dose of 12.6 Gy-Eq. The protocols of the third and fourth groups allowed the prescribed peak normal brain dose of 12.6 Gy-Eq to be outside of the tumor volume, used a 12-cm collimator and, respectively, uni- or bilateral irradiations. We describe the treatment planning procedures and report the doses delivered to various structures of the brain.

  7. Neurological damage disrupts normal sex differences in psychophysiological responsiveness to music.

    Science.gov (United States)

    Belfi, Amy M; Chen, Kuan-Hua; Schneider, Brett; Tranel, Daniel

    2016-01-01

    Men and women often display different physiological responses to emotional stimuli, and these responses can be affected by brain damage. Here, we investigated how brain damage differentially affects electrodermal responses based on sex. We studied neurologically normal, healthy adults and a sample of neurological patients. Participants listened to music, an emotional stimulus that reliably elicits skin conductance responses (SCRs). Electrodermal activity was recorded while participants listened to musical clips. When analyzing the data without regard to sex, there were no differences between healthy and brain-damaged participants in their SCRs. However, we found a significant interaction between brain injury status and sex. For men, brain damage significantly reduced SCRs. For women, there were no differences between brain-damaged participants and neurologically healthy participants. These findings illustrate the importance of including demographic variables, such as sex, when investigating brain-behavior relationships with a psychophysiological dependent variable.

  8. NORMALIZED DIFFERENCE SNOW INDEX SIMULATION FOR SNOW-COVER MAPPING IN FOREST BY GEOSAIL MODEL

    Institute of Scientific and Technical Information of China (English)

    CAO Yun-gang; LIU Chuang

    2006-01-01

    The snow-cover mapping in forest area is always one of the difficult points for optical satellite remote sensing. To investigate reflectance variability and to improve the mapping of snow in forest area, GeoSail model was used to simulate the reflectance of a snow-covered forest. Using this model, the effects of varying canopy density, solar illumination and view geometry on the performance of the MODIS (Moderate-resolution Imaging Spectroradiometer)snow-cover mapping algorithm were investigated. The relationship between NDSI (Normalized Difference Snow Index), NDVI (Normalized Difference Vegetation Index) and snow fraction was discussed in detail. Results indicated that the weak performance would be achieved if fixed criteria were used for different regions especially in the complicated land cover components. Finally, some suggestions to MODIS SNOWMAP algorithm were put forward to improve snow mapping precision in forest area based on the simulation, for example, new criteria should be used in coniferous forest, that is, NDSI greater than 0.3 and NDVI greater than zero. Otherwise, a threshold on view zenith angle may be used in the criteria such as 45°.

  9. Driver Gaze Behavior Is Different in Normal Curve Driving and when Looking at the Tangent Point.

    Directory of Open Access Journals (Sweden)

    Teemu Itkonen

    Full Text Available Several steering models in the visual science literature attempt to capture the visual strategies in curve driving. Some of them are based on steering points on the future path (FP, others on tangent points (TP. It is, however, challenging to differentiate between the models' predictions in real-world contexts. Analysis of optokinetic nystagmus (OKN parameters is one useful measure, as the different strategies predict measurably different OKN patterns. Here, we directly test this prediction by asking drivers to either a "drive as they normally would" or b to "look at the TP". The design of the experiment is similar to a previous study by Kandil et al., but uses more sophisticated methods of eye-movement analysis. We find that the eye-movement patterns in the "normal" condition are indeed markedly different from the "tp" condition, and consistent with drivers looking at waypoints on the future path. This is the case for both overall fixation distribution, as well as the more informative fixation-by-fixation analysis of OKN. We find that the horizontal gaze speed during OKN corresponds well to the quantitative prediction of the future path models. The results also definitively rule out the alternative explanation that the OKN is produced by an involuntary reflex even while the driver is "trying" to look at the TP. The results are discussed in terms of the sequential organization of curve driving.

  10. Different Sensitivities to Apoptotic Induction by Camptothecin between Normal and Senescent Lens Epithelial Cells

    Institute of Scientific and Technical Information of China (English)

    Haike Guo; Haiying Jin; Liya Wang; Hongyang Zhang; Xin Yang

    2002-01-01

    Purpose: To investigate whether normal and senescent lens epithelial cells have different defense abilities to apoptotic induction factor in vitro.Methods: Rabbit lens epithelial cells were cultured, passed. When reaching confluence, cells from the first and seventh passage were stained by x-gal staining to detect cell senescence. Cell apoptosis was detected by TUNEL(Roche).10μmol/L camptothecin was used to induce cell apoptosis from the lens epithelial cells of the first and seventh passage to distinguish different sensitivities to apoptotic induction factor between normal and senescent cells.Results: The senescent cells (41.17% ± 5.24% ) were detected in the lens epithelial cell culture of the seventh passage, which are higher than those of the first passage (0.98% ±0. 39% ). There was no apoptotic cell detected in the cell cultures undisturbed. Exposure of the first passage cells to camptothecin resulted in death of approximately 23.87% ± 3.45% of the cells during a 36 hour exposure period. In contrast, significantly more lens epithelial cells died through the apoptosis (38.29% ±4. 01% ) from the seventh passage.Conclusion: Senescent cells increased with cell passage. Senescence lens epithelial cells do not undergo apoptosis if they were not disturbed. But the vulnerabilities to apoptotic induction between health and senescence cells were different.

  11. GENDER DIFFERENCES IN FINE AND GROSS MOTOR SKILLS OF NORMALLY DEVELOPING OVERWEIGHT AND OBESE ADOLESCENTS

    Directory of Open Access Journals (Sweden)

    Şehmus ASLAN

    2016-12-01

    Full Text Available The purpose of this research is to compare girls’ and boys’ fine and gross motor skills in normally developing overweight and obese adolescents. 18 girls and 28 boys with 12-18 age range, in totally 46 overweight and obese adolecents participated in the study. Body mass index of participants were calculated. Fine and gross motor skills of adolescents were assessed by Short Form Bruininks-Oseretsky Test of Motor Proficiency (BOT-2 SF. There was no differences between girls and boys for BOT-2 SF total scores (p>0.05, but it was found differences in several items of BOT-2 SF. Scores of test items for fine motor scores in the girls were higher than the boys (p0.05. Gross motor skill tests including jumping in place, dribling a ball, sit-ups, one- legged stationary hop test scores were higher in the boys, while tapping feet and fingers test score was higher in the girls (p<0.05. Our results suggested that there is differences between girls’ and boys’ fine and gross motor skills in normally developing overweight and obese adolescents. Girls have better fine motor skill performance while boys have better gross motor skill performance in overweight and obese adolescents.

  12. Multifractal Downscaling of Rainfall Using Normalized Difference Vegetation Index (NDVI) in the Andes Plateau

    Science.gov (United States)

    Posadas, A. N.; Carbajal, M.; Quiroz, R.

    2017-01-01

    In this paper, a multifractal downscaling technique is applied to adequately transformed and lag corrected normalized difference vegetation index (NDVI) in order to obtain daily estimates of rainfall in an area of the Peruvian Andean high plateau. This downscaling procedure is temporal in nature since the original NDVI information is provided at an irregular temporal sampling period between 8 and 11 days, and the desired final scale is 1 day. The spatial resolution of approximately 1 km remains the same throughout the downscaling process. The results were validated against on-site measurements of meteorological stations distributed in the area under study. PMID:28125607

  13. Multifractal Downscaling of Rainfall Using Normalized Difference Vegetation Index (NDVI) in the Andes Plateau.

    Science.gov (United States)

    Duffaut Espinosa, L A; Posadas, A N; Carbajal, M; Quiroz, R

    2017-01-01

    In this paper, a multifractal downscaling technique is applied to adequately transformed and lag corrected normalized difference vegetation index (NDVI) in order to obtain daily estimates of rainfall in an area of the Peruvian Andean high plateau. This downscaling procedure is temporal in nature since the original NDVI information is provided at an irregular temporal sampling period between 8 and 11 days, and the desired final scale is 1 day. The spatial resolution of approximately 1 km remains the same throughout the downscaling process. The results were validated against on-site measurements of meteorological stations distributed in the area under study.

  14. Sex differences in obesity and cognitive function in a cognitively normal aging Chinese Han population

    Directory of Open Access Journals (Sweden)

    Li W

    2017-09-01

    Full Text Available Wei Li,* Qi Qiu,* Lin Sun, Ling Yue, Tao Wang, Xia Li, Shifu Xiao Alzheimer’s Disease and Related Disorders Center, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work Background: Sex differences in Alzheimer’s disease and mild cognitive impairment have been well recognized. However, sex differences in cognitive function and obesity in cognitively normal aging Chinese Han population have not attracted much attention. Objective: The aim of this study was to investigate the relationship between sex, obesity, and cognitive function in an elderly Chinese population with normal cognitive function. Subjects and methods: A total of 228 cognitively normal aging participants (males/females =93/135 entered this study. Their general demographic information (sex, age, and education was collected by standardized questionnaire. Apolipoprotein E (APOE genotype and serum lipid levels were measured. The Montreal Cognitive Assessment (MoCA was used to assess participants’ cognitive function. Results: The prevalence of obesity in elderly women (18/133, 13.5% was significantly higher than that in men (5/92, 5.4%, P=0.009. Regression analyses showed that obesity was associated with drinking alcohol (OR =13.695, P=0.045 and triglyceride (OR =1.436, P=0.048 in women and limited to low-density lipoprotein (OR =11.829, P=0.023 in men. Women performed worse on the naming score for MoCA than men (P<0.01. Stepwise linear regression analysis showed that education (t=3.689, P<0.001 and smoking (t=2.031, P=0.045 were related to the score of naming in female, while high-density lipoprotein (t=–2.077, P=0.041 was related to the score of naming in male; however, no correlation was found between body mass index and cognitive function in both male and female (P>0.05. Conclusion: Our finding suggests that there are significant sex differences in obesity and

  15. Variability and systematic differences in normal, protan, and deutan color naming.

    Science.gov (United States)

    Nagy, Balázs V; Németh, Zoltán; Samu, Krisztián; Ábrahám, György

    2014-01-01

    The congenital color vision deficient (CVD) generally demonstrates difficulties in color naming tasks. In our study we investigated color naming properties and uncertainties of a relatively large group of red-green CVDs using quasi monochromatic stimuli and seven basic color terms. The results show a large variability in color naming for the CVD when contrasted to normal color vision and similar alterations when comparing protans to deutans. Statistically significant differences were found in specific wavelength ranges between the tested groups. In general, protans and deutans have shown better color naming ability than expected, which suggests the use of non-chromatic visual cues.

  16. Difference in normal corneal thickness and curvature between Mongolian and Han nationalities

    Institute of Scientific and Technical Information of China (English)

    Hai-Xia; Zhao; Li; Zhang; Wen-Ying; Guan

    2015-01-01

    AIM: To investigate the differences in central corneal thickness(CCT) and curvature in myopic patients with different genders between Mongolian and Han nationalities in the Inner Mongolia region.METHODS: Patients with myopia, among whom 122cases(244 eyes) were Mongolian and 150 cases(300eyes) were Han, were selected. Pentacam was used to measure the corneal curvature, whereas CCT was determined by Pentacam, Orbscan and ultrasound pachymetry.RESULTS: Comparisons of the curvature of men and women in Mongolia showed a significant difference(P <0.05) by comparing of the CCT values measured by three methods in Han and Mongolian in Inner Mongolia, we can draw a conclusion that the CCT values measured by Pentacam are less than the values by Orbscan and ultrasound pachymetry, and there are statistically significant difference in CCT measurement among the instruments except between the Orbscan and ultrasound pachymetry. The CCT values of Mongolians are more than Han people, and the analysis exhibited statistical significance. The CCT values measured by Orbscan in Mongolia of women are more than men, the difference showed statistical significance.CONCLUSION: Corneal curvature in Mongolian females was significantly higher than that in males. However, no significant difference was observed among Han males and females. The CCT values of Mongolian females were higher than those of males. The normal CCT values measured by Pentacam in Mongolian myopic patients were smaller than those obtained by Orbscan or ultrasound. The normal CCT values of Mongolian patients with myopia were higher than those reported for domestic people.

  17. Differences in antiepileptic drug efficacy in hippocampally kindled normal and microcephalic rats.

    Science.gov (United States)

    Majkowski, J; Danneberg, P; Knappen, F; Sersen, E A

    1986-10-29

    The difference in antiepileptic drug efficacy was investigated in two groups of animals: 5 normal and 4 microcephalic rats. The latter were produced by a single i.p. injection of 30 mg/kg methylazoxymethanol acetate in the mother on the 15th day of gestation. Hippocampal kindling was performed to a seizure criterion in all animals followed by testing of the antiepileptic drugs vs placebo. Besides carbamazepine (CBZ), two new anticonvulsants were tested: (E)-2-[(alpha-amino)phenylmethylene]-benzo-[b]-thiophene-3(2H)-one (AF-CX 921) and its metabolite (E)-2-[alpha-amino)phenylmethylene]-benzo-[b]-thiophene-3(2H)-one- 1- oxide (AF-CX 1325). Frequency of occurrence and duration of afterdischarges and seizures were statistically examined. The duration of early afterdischarges (AD1) tended to be shorter in microcephalic than in normal animals in control and placebo periods. In contrast, during treatment with the antiepileptic drugs, AD1 durations were longer in microcephalic than in normal animals. This suggests that the drugs inhibited AD1 to a lesser extent in the microcephalics. Two other characteristics of EEG epileptic activity, focal spiking (FS) and late afterdischarges (AD2) also varied in the two groups. Both were significantly lower in occurrence in the microcephalic rats independent of treatment. Three types of behavioral manifestations were also examined: convulsive seizures (CS), epileptic behavior (EB) and quiet states (Q). The two groups of animals responded differently to the drugs with respect to Q and CS. In the microcephalics, AFCX 1325 and AFCX 921 were superior to CBZ, which in turn, was superior to placebo.(ABSTRACT TRUNCATED AT 250 WORDS)

  18. Shear viscosity and normal pressure differences in the scope of the extended Kirkwood-Smoluchowski equation

    Science.gov (United States)

    Koo, H.-M.

    1993-03-01

    The potential contribution to the viscosity in liquids is calculated from the pair-correlation function, which may be obtained by solving the extended Kirkwood-Smoluchowski (KS) equation. A secondary boundary condition near the hard core (r=1+) for the excess pair probability current density in the relative pair space is derived rigorously and applied to the extended KS equation. The intermolecular potential consists of hard core plus arbitrary soft tail. The viscosity coefficients calculated in this work prove to be essentially functions of the square root of the shear rate rather than functions of the shear rate itself. We give the explicit representation for the viscosity coefficients in the case of hard spheres. The shear thinning of the shear viscosity is recovered. The viscosity of the normal pressure difference 1/2(pxx-pyy) is found to be positive. The normal pressure difference of the second kind 1/2[pzz-1/2(pxx+pyy)] vanishes in the first-order perturbation calculation with respect to the deformation.

  19. Creatinine normalization of workplace urine drug tests: does it make a difference?

    Science.gov (United States)

    Price, James W

    2013-01-01

    This study examines the effect of creatinine normalization on urine drug concentrations of 5 substances (amphetamines, cocaine, marijuana, opiates, and phencyclidine) and how this affects the proportion of reported positives. The Wilcoxon matched-pairs signed-ranks test was used to compare the mean prenormalization urinary drug concentration with the mean postnormalization urinary drug concentration. Frequency analysis was performed on dichotomous drug test results and the information was used to complete McNemar testing for each drug to determine the difference of proportions for prenormalization positive drug tests to postnormalization positive drug test. Each drug tested (N = 4460) was found to have a statistically significant increase in mean urinary drug concentration after creatinine normalization with effect sizes ranging from small to medium with cocaine having the largest effect size (r = 0.229) and phencyclidine having the lowest effect size (r = 0.121). The differences in proportion of dichotomous results between study and control groups for drugs tested were compared with the McNemar test. Each drug had a statistically significant (P = 0.0010) increase of positive drug tests. This result indicates that specimen dilution does affect the number of laboratory-positive results confirmed.

  20. Desertification Risk Monitoring for North Shaanxi Province, China, Using Normalized Difference Vegetation Index (NDVI)

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    In this study, the remote sensing is applied to the examination of the relationship between desertification and normalized difference vegetation index (NDVI) in the context of northern Shaanxi Province. This relationship is also examined using spatial analysis methods. A strong negative correlation is found in the largest area desert, indicating that the relationship between desert and NDVI is not a simple linear one and that the correlation coefficient between NDVI and vegetation abundance is significant.The normalized difference vegetation index (NDVI) was compared with other vegetation index-based methodologies. NDVI is a valuable first-cut indicator for such systems, although the analysis and interpretation of its relationship to desertification are complex and also based on the detailed analysis of its reiationship to ecological zone, vegetation type and season. Conclusions thus made would help to upgrade the methodology as an effective tool for early-warning desertification in the northern Shaanxi Province where a drought is a recurring threat. This methodology includes the integration of NDVI with other socio-economic and bio-physical indicators in GIS, the complementation of desert area data with satellite data, and the analysis of the relationship between NDVI and specific climatic zones, for each season and vegetation type.

  1. RADIOMETRIC NORMALIZATION OF LARGE AIRBORNE IMAGE DATA SETS ACQUIRED BY DIFFERENT SENSOR TYPES

    Directory of Open Access Journals (Sweden)

    S. Gehrke

    2016-06-01

    Full Text Available Generating seamless mosaics of aerial images is a particularly challenging task when the mosaic comprises a large number of im-ages, collected over longer periods of time and with different sensors under varying imaging conditions. Such large mosaics typically consist of very heterogeneous image data, both spatially (different terrain types and atmosphere and temporally (unstable atmo-spheric properties and even changes in land coverage. We present a new radiometric normalization or, respectively, radiometric aerial triangulation approach that takes advantage of our knowledge about each sensor’s properties. The current implementation supports medium and large format airborne imaging sensors of the Leica Geosystems family, namely the ADS line-scanner as well as DMC and RCD frame sensors. A hierarchical modelling – with parameters for the overall mosaic, the sensor type, different flight sessions, strips and individual images – allows for adaptation to each sensor’s geometric and radiometric properties. Additional parameters at different hierarchy levels can compensate radiome-tric differences of various origins to compensate for shortcomings of the preceding radiometric sensor calibration as well as BRDF and atmospheric corrections. The final, relative normalization is based on radiometric tie points in overlapping images, absolute radiometric control points and image statistics. It is computed in a global least squares adjustment for the entire mosaic by altering each image’s histogram using a location-dependent mathematical model. This model involves contrast and brightness corrections at radiometric fix points with bilinear interpolation for corrections in-between. The distribution of the radiometry fixes is adaptive to each image and generally increases with image size, hence enabling optimal local adaptation even for very long image strips as typi-cally captured by a line-scanner sensor. The normalization approach is implemented in

  2. Content of Androgen Receptor in Cultured Genital Skin Fibroblast From Different Ages of Chinese Normal Men

    Institute of Scientific and Technical Information of China (English)

    卢建; 何立敏; 张金山; 杨震; 周云

    1995-01-01

    A ratpid, simple, reliable method is described for assaying androgen receptor (AR) in dispersed, whole, cultured human genital skin fibroblasts (GSF) with a synthetic androgen, 3H-methyltrienolone (3H-R1881). Receptors for androgen in GSF exhiblt high affinity (Kd=3.0±0.1 nmol/L), low binding capacity and androgen specificity. The content of AR in cultured GSF from 40 normal men varying in age from 1.5—60 years u:as also investigated by this assay. Scatchard analysis and slngle plot revealed the presence of 4.500-8500 binding sites per cell, mean number of AR in GSF of these men is 6288±1082 binding sites/cell. No significant difference was observed in the content of AR in different age groups. This result showed that the content of AR in these ceils did not change with age.

  3. Assessing Disagreement and Tolerance of Misclassification of Satellite-derived Land Cover Products Used in WRF Model Applications

    Institute of Scientific and Technical Information of China (English)

    GAO Hao; JIA Gensuo

    2013-01-01

    As more satellite-derived land cover products used in the study of global change,especially climate modeling,assessing their quality has become vitally important.In this study,we developed a distance metric based on the parameters used in weather research and forecasting (WRF) to characterize the degree of disagreement among land cover products and to identify the tolerance for misclassification within the International Geosphere Biosphere Programme (IGBP) classification scheme.We determined the spatial degree of disagreement and then created maps of misclassification of Moderate Resolution Imaging Spectoradiometer (MODIS) products,and we calculated overall and class-specific accuracy and fuzzy agreement in a WRF model.Our results show a high level of agreement and high tolerance of misclassification in the WRF model between large-scale homogeneous landscapes,while a low level of agreement and tolerance of misclassification appeared in heterogeneous landscapes.The degree of disagreement varied significantly among seven regions of China.The class-specific accuracy and fuzzy agreement in MODIS Collection 4 and 5 products varied significantly.High accuracy and fuzzy agreement occurred in the following classes:water,grassland,cropland,and barren or sparsely vegetated.Misclassification mainly occurred among specific classes with similar plant functional types and low discriminative spectro-temporal signals.Some classes need to be improved further; the quality of MODIS land cover products across China still does not meet the common requirements of climate modeling.Our findings may have important implications for improving land surface parameterization for simulating climate and for better understanding the influence of the land cover change on climate.

  4. Assessment of Satellite-Derived Essential Climate Variables in the Terrestrial Domain: Overview and Status of the CEOS LPV Subgroup

    Science.gov (United States)

    Roman, M. O.

    2015-12-01

    The validation of satellite-derived terrestrial observations has perennially faced the challenge of finding a consistent set of in-situ measurements that can both cover a wide range of surface conditions and provide timely and traceable product accuracy and uncertainty information. The Committee on Earth Observation Satellites (CEOS), the space arm of the Group on Earth Observations (GEO), plays a key role in coordinating the land product validation process. The Land Product Validation (LPV) sub-group of the CEOS Working Group on Calibration and Validation (WGCV) aims to address the challenges associated with the validation of global land products. This paper will provide a status of LPV subgroup focus area activities, which cover seven Global Climate Observing System (GCOS) terrestrial Essential Climate Variables (ECVs): (1) Snow Cover, (2) Surface Albedo, (3) Land Cover, (4) Leaf Area Index, (5) Fraction of Absorbed Photosynthetically Active Radiation (FAPAR), (6) Active Fires, and (7) Soil Moisture; as well as two additional variables (Land Surface Phenology and Land Surface Temperature), which are deemed of high priority of the LPV community. A primary focus of LPV is the implementation of a global validation framework for product intercomparison and validation (fig. 1). This framework is based on a citable protocol, fiducial reference data, and automated subsetting. Ideally, each of these parts will be integrated into an online platform where quantitative tests are run, and standardized intercomparison and validation results reported for all products used in the validation exercise. The establishment of consensus guidelines for in situ measurements as well as inter-comparison of trends derived from independently-obtained reference data and derived products will enhance coordination of the scientific needs of the Earth system communities with global LPV activities (http://lpvs.gsfc.nasa.gov/).

  5. Normalization method for asphalt mixture fatigue equation under different loading frequencies

    Institute of Scientific and Technical Information of China (English)

    吕松涛; 郑健龙

    2015-01-01

    In order to analyze the effect of different loading frequencies on the fatigue performance for asphalt mixture, the changing law of asphalt mixture strengths with loading speed was revealed by strength tests under different loading speeds. Fatigue equations of asphalt mixtures based on the nominal stress ratio and real stress ratio were established using fatigue tests under different loading frequencies. It was revealed that the strength of the asphalt mixture is affected by the loading speed greatly. It was also discovered that the fatigue equation based on the nominal stress ratio will change with the change of the fatigue loading speed. There is no uniqueness. But the fatigue equation based on the real stress ratio doesn’t change with the loading frequency. It has the uniqueness. The results indicate the fatigue equation based on the real stress ratio can realize the normalization of the asphalt mixture fatigue equation under different loading frequencies. It can greatly benefit the analysis of the fatigue characteristics under different vehicle speeds for asphalt pavement.

  6. Breast cancer subtype distribution is different in normal weight, overweight, and obese women.

    Science.gov (United States)

    Gershuni, Victoria; Li, Yun R; Williams, Austin D; So, Alycia; Steel, Laura; Carrigan, Elena; Tchou, Julia

    2017-06-01

    Obesity is associated with tumor promoting pathways related to insulin resistance and chronic low-grade inflammation which have been linked to various disease states, including cancer. Many studies have focused on the relationship between obesity and increased estrogen production, which contributes to the pathogenesis of estrogen receptor-positive breast cancers. The link between obesity and other breast cancer subtypes, such as triple-negative breast cancer (TNBC) and Her2/neu+ (Her2+) breast cancer, is less clear. We hypothesize that obesity may be associated with the pathogenesis of specific breast cancer subtypes resulting in a different subtype distribution than normal weight women. A single-institution, retrospective analysis of tumor characteristics of 848 patients diagnosed with primary operable breast cancer between 2000 and 2013 was performed to evaluate the association between BMI and clinical outcome. Patients were grouped based on their BMI at time of diagnosis stratified into three subgroups: normal weight (BMI = 18-24.9), overweight (BMI = 25-29.9), and obese (BMI > 30). The distribution of breast cancer subtypes across the three BMI subgroups was compared. Obese and overweight women were more likely to present with TNBC and normal weight women with Her2+ breast cancer (p = 0.008). We demonstrated, for the first time, that breast cancer subtype distribution varied significantly according to BMI status. Our results suggested that obesity might activate molecular pathways other than the well-known obesity/estrogen circuit in the pathogenesis of breast cancer. Future studies are needed to understand the molecular mechanisms that drive the variation in subtype distribution across BMI subgroups.

  7. Elastic microfibril distribution in the cornea: Differences between normal and keratoconic stroma.

    Science.gov (United States)

    White, Tomas L; Lewis, Philip N; Young, Robert D; Kitazawa, Koji; Inatomi, Tsutomu; Kinoshita, Shigeru; Meek, Keith M

    2017-06-01

    The optical and biomechanical properties of the cornea are largely governed by the collagen-rich stroma, a layer that represents approximately 90% of the total thickness. Within the stroma, the specific arrangement of superimposed lamellae provides the tissue with tensile strength, whilst the spatial arrangement of individual collagen fibrils within the lamellae confers transparency. In keratoconus, this precise stromal arrangement is lost, resulting in ectasia and visual impairment. In the normal cornea, we previously characterised the three-dimensional arrangement of an elastic fiber network spanning the posterior stroma from limbus-to-limbus. In the peripheral cornea/limbus there are elastin-containing sheets or broad fibers, most of which become microfibril bundles (MBs) with little or no elastin component when reaching the central cornea. The purpose of the current study was to compare this network with the elastic fiber distribution in post-surgical keratoconic corneal buttons, using serial block face scanning electron microscopy and transmission electron microscopy. We have demonstrated that the MB distribution is very different in keratoconus. MBs are absent from a region of stroma anterior to Descemet's membrane, an area that is densely populated in normal cornea, whilst being concentrated below the epithelium, an area in which they are absent in normal cornea. We contend that these latter microfibrils are produced as a biomechanical response to provide additional strength to the anterior stroma in order to prevent tissue rupture at the apex of the cone. A lack of MBs anterior to Descemet's membrane in keratoconus would alter the biomechanical properties of the tissue, potentially contributing to the pathogenesis of the disease. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  8. Switchable different operation states in an erbium-doped fiber laser cavity with normal dispersion

    Science.gov (United States)

    Gao, Weiqing; Liao, Meisong; Kawashima, Hiroyasu; Suzuki, Takenobu; Ohishi, Yasutake

    2012-08-01

    We demonstrate different operation states which can be switched by the polarization control in an erbium-doped fiber laser cavity with normal dispersion, including passive single-pulse and multiple-pulse mode-locking, coherent pulse pattern, and passive Q-switching. The mode-locked single pulse has a smooth and broad rectangular-shaped spectrum. With increasing pump power, multiple pulses appear and finally six pulses are observed, where the pulses have no interaction with each other. Keeping the pump power at 407 mW and adjusting the polarization state, we observe the coherent pulse pattern with the pulse numbers from 2 to 5. It is the first time five coherent pulses in the 1.55 μm normal dispersion cavity have been observed, to our knowledge. The mode-locked spectra are highly modulated and the largest pulse separation of 31.9 ps is observed for the two-pulse case. When the pump power exceeds 180 mW, the mode-locked operation can be switched to the passively Q-switched operation by controlling the polarization state. The repetition rate and pulse width can be changed by pump power variation, and the spectrum is tunable in the range of 8.45 nm.

  9. Do normal pupil diameter differences in the population underlie the color selection of #thedress?

    Science.gov (United States)

    Vemuri, Kavita; Bisla, Kulvinder; Mulpuru, SaiKrishna; Varadharajan, Srinivasa

    2016-03-01

    The fundamental question that arises from the color conundrum of #thedress is "What are the phenomena that underlie the individual differences in the reported colors when all other conditions like light and device for the display are identical?" A survey of 384 participants showed near-equal distribution into blue/black (b/b) and white/gold (w/g) groups. We looked at pupil size differences in a sample population of 53 individuals from these two groups and a group that switched from w/g to b/b. Our results showed that the w/g and switch groups had significantly lower pupil size than the b/b group (w/gpupil size. A standard infinity focus experiment was then conducted on 18 participants from each group to check if there is bimodality in the population, and we found a statistically significant difference (w/gpupil size by 3-4 mm, but the participants did not report a switch. The results suggest a population difference in normal pupil size influencing color observation which was exposed by the dress.

  10. Principle and geomorphological applicability of summit level and base level technique using Aster Gdem satellite-derived data and the original software Baz

    OpenAIRE

    Akihisa Motoki; Kenji Freire Motoki; Susanna Eleonora Sichel; Samuel da Silva; José Ribeiro Aires

    2015-01-01

    This article presents principle and geomorphological applicability of summit level technique using Aster Gdem satellite-derived topographicdata. Summit level corresponds to thevirtualtopographic surface constituted bylocalhighest points, such as peaks and plateau tops, and reconstitutes palaeo-geomorphology before the drainage erosion. Summit level map is efficient for reconstitution of palaeo-surfaces and detection of active tectonic movement. Base level is thevirtualsurface composed oflocal...

  11. Alpha particle induced DNA damage and repair in normal cultured thyrocytes of different proliferation status

    DEFF Research Database (Denmark)

    Lyckesvärd, Madeleine Nordén; Delle, Ulla; Kahu, Helena

    2014-01-01

    mechanism as (131)I [1], in cancer treatment has increased during recent years because of its high efficiency in inducing biological damage and beneficial dose distribution when compared to low-LET radiation. Most knowledge of the DNA damage response in thyroid is from studies using low-LET irradiation...... and much less is known of high-LET irradiation. In this paper we investigated the DNA damage response and biological consequences to photons from Cobolt-60 ((60)Co) and alpha particles from (211)At in normal primary thyrocytes of different cell cycle status. For both radiation qualities the intensity....... Increasing ratios of micronuclei per cell nuclei were seen up to 1Gy (211)At. We found that primary thyrocytes were much more sensitive to alpha particle exposure compared with low-LET photons. Calculations of the relative biological effectiveness yielded higher RBE for cycling cells compared with stationary...

  12. A Normalized Difference Vegetation Index (NDVI Time-Series of Idle Agriculture Lands: A Preliminary Study

    Directory of Open Access Journals (Sweden)

    Andrew K Skidmore

    2011-01-01

    Full Text Available In this paper, the NDVI time-series collected from the study area between year 2003 and 2005 of all land cover types are plotted and compared. The study area is the agricultural zones in Banphai District, Khonkean, Thailand. The LANDSAT satellite images of different dates were first transformed into a time series of Normalized Difference Vegetation Index (NDVI images before the investigation. It can be visually observed that the NDVI time series of the Idle Agriculture Land (IAL has the NDVI values closed to zero. In other words, the trend of the NDVI values remains, approximately, unchanged about the zero level for the whole period of the study time. In contrast, the non-idle areas hold a higher level of the NDVI variation. The NDVI values above 0.5 can be found in these non-idle areas during the growing seasons. Thus, it can be hypothesized that the NDVI time-series of the different land cover types can be used for IAL classification. This outcome is a prerequisite to the follow-up study of the NDVI pattern classification that will be done in the near future.

  13. Snow Monitoring Using Remote Sensing Data: Modification of Normalized Difference Snow Index

    Science.gov (United States)

    Kaplan, G.; Avdan, U.

    2016-12-01

    Snow cover is an important part of the Earth`s climate system so its continuous monitoring is necessary to map snow cover in high resolution. Satellite remote sensing can successfully fetch land cover and land cover changes. Although normalized difference snow index NDSI has quite good accuracy, topography shadow, water bodies and clouds can be easily misplaced as snow. Using Landsat TM, +ETM and TIRS/OLI satellite images, the NDSI was modified for more accurate snow mapping. In this paper, elimination of the misplaced water bodies was made using the high reflectance of the snow in the blue band. Afterwards, the modified NDSI (MNDSI) was used for estimating snow cover through the years on the highest mountains in Republic of Macedonia. The results from this study show that the MNDSI accuracy is bigger than the NDSI`s, totally eliminating the misplaced water bodies, and partly the one caused from topography and clouds. Also, it was noticed that the snow cover in the study area has been lowered through the years. For future studies, the MNDSI should be validated on different study areas with different characteristics.

  14. Impact of Different Normality Thresholds for 24-hour ABPM at the Primary Health Care Level

    Science.gov (United States)

    Grezzana, Guilherme Brasil; Moraes, David William; Stein, Airton Tetelbon; Pellanda, Lucia Campos

    2017-01-01

    Background Hypertension is an important risk factor for cardiovascular outcomes. Primary health care (PHC) physicians should be prepared to act appropriately in the prevention of cardiovascular risk factors. However, the rates of patients with control of blood pressure (BP) remain low. The impact of the reclassification of high BP by 24-hour ambulatory BP monitoring (ABPM) can lead to different medical decisions in PHC. Objective To evaluate the agreement between the BP measured by a conventional method by PHC physicians and by 24-hour ABPM, considering different BP normal thresholds for the 24-hour ABPM according to the V Brazilian ABPM Guidelines and the European Society of Hypertension Guidelines. Methods A cross-sectional study including 569 hypertensive patients. The BP was initially measured by the PHC physicians and, later, by 24-hour ABPM. The BP measurements were obtained independently between the two methods. The therapeutic targets for the conventional BP followed the guidelines by the Eighth Joint National Committee (JNC 8), the V ABPM Brazilian Guidelines, and the 2013 European Hypertension Guidelines. Results There was an accuracy of 54.8% (95% confidence interval [95%CI] 0.51 - 0.58%) for the BP measured with the conventional method when compared with the 24-hour ABPM, with a sensitivity of 85% (95%CI 80.8 - 88.6%), specificity of 31.9% (95%CI 28.7 - 34.7%), and kappa value of 0.155, when considering the European Hypertension Guidelines. When using more stringent thresholds to characterize the BP as "normal" by ABPM, the accuracy was 45% (95%CI 0.41 - 0.47%) for conventional measurement when compared with 24-hour ABPM, with a sensitivity of 86.7% (95%CI 0.81 - 0.91%), specificity of 29% (95%CI 0.26 - 0.30%), and kappa value of 0.103. Conclusion The BP measurements obtained by PHC physicians showed low accuracy when compared with those obtained by 24-hour ABPM, regardless of the threshold set by the different guidelines. PMID:28099585

  15. Impact of Different Normality Thresholds for 24-hour ABPM at the Primary Health Care Level

    Directory of Open Access Journals (Sweden)

    Guilherme Brasil Grezzana

    Full Text Available Abstract Background: Hypertension is an important risk factor for cardiovascular outcomes. Primary health care (PHC physicians should be prepared to act appropriately in the prevention of cardiovascular risk factors. However, the rates of patients with control of blood pressure (BP remain low. The impact of the reclassification of high BP by 24-hour ambulatory BP monitoring (ABPM can lead to different medical decisions in PHC. Objective: To evaluate the agreement between the BP measured by a conventional method by PHC physicians and by 24-hour ABPM, considering different BP normal thresholds for the 24-hour ABPM according to the V Brazilian ABPM Guidelines and the European Society of Hypertension Guidelines. Methods: A cross-sectional study including 569 hypertensive patients. The BP was initially measured by the PHC physicians and, later, by 24-hour ABPM. The BP measurements were obtained independently between the two methods. The therapeutic targets for the conventional BP followed the guidelines by the Eighth Joint National Committee (JNC 8, the V ABPM Brazilian Guidelines, and the 2013 European Hypertension Guidelines. Results: There was an accuracy of 54.8% (95% confidence interval [95%CI] 0.51 - 0.58% for the BP measured with the conventional method when compared with the 24-hour ABPM, with a sensitivity of 85% (95%CI 80.8 - 88.6%, specificity of 31.9% (95%CI 28.7 - 34.7%, and kappa value of 0.155, when considering the European Hypertension Guidelines. When using more stringent thresholds to characterize the BP as "normal" by ABPM, the accuracy was 45% (95%CI 0.41 - 0.47% for conventional measurement when compared with 24-hour ABPM, with a sensitivity of 86.7% (95%CI 0.81 - 0.91%, specificity of 29% (95%CI 0.26 - 0.30%, and kappa value of 0.103. Conclusion: The BP measurements obtained by PHC physicians showed low accuracy when compared with those obtained by 24-hour ABPM, regardless of the threshold set by the different guidelines.

  16. Satellite Derived Water Quality Observations Are Related to River Discharge and Nitrogen Loads in Pensacola Bay, Florida

    Directory of Open Access Journals (Sweden)

    John C. Lehrter

    2017-09-01

    Full Text Available Relationships between satellite-derived water quality variables and river discharges, concentrations and loads of nutrients, organic carbon, and sediments were investigated over a 9-year period (2003–2011 in Pensacola Bay, Florida, USA. These analyses were conducted to better understand which river forcing factors were the primary drivers of estuarine variability in several water quality variables. Remote sensing reflectance time-series data were retrieved from the MEdium Resolution Imaging Spectrometer (MERIS and used to calculate monthly and annual estuarine time-series of chlorophyll a (Chla, colored dissolved organic matter (CDOM, and total suspended sediments (TSS. Monthly MERIS Chla varied from 2.0 mg m−3 in the lower region of the bay to 17.2 mg m−3 in the upper bay. MERIS CDOM and TSS exhibited similar patterns with ranges of 0.51–2.67 (m−1 and 0.11–8.9 (g m−3. Variations in the MERIS-derived monthly and annual Chla, CDOM, and TSS time-series were significantly related to monthly and annual river discharge and loads of nitrogen, organic carbon, and suspended sediments from the Escambia and Yellow rivers. Multiple regression models based on river loads (independent variables and MERIS Chla, CDOM, or TSS (dependent variables explained significant fractions of the variability (up to 62% at monthly and annual scales. The most significant independent variables in the regressions were river nitrogen loads, which were associated with increased MERIS Chla, CDOM, and TSS concentrations, and river suspended sediment loads, which were associated with decreased concentrations. In contrast, MERIS water quality variations were not significantly related to river total phosphorus loads. The spatially synoptic, nine-year satellite record expanded upon the spatial extent of past field studies to reveal previously unseen system-wide responses to river discharge and loading variation. The results indicated that variations in Pensacola Bay Chla

  17. Potential for a biogenic influence on cloud microphysics over the ocean: a correlation study with satellite-derived data

    Directory of Open Access Journals (Sweden)

    A. Lana

    2012-09-01

    Full Text Available Aerosols have a large potential to influence climate through their effects on the microphysics and optical properties of clouds and, hence, on the Earth's radiation budget. Aerosol–cloud interactions have been intensively studied in polluted air, but the possibility that the marine biosphere plays an important role in regulating cloud brightness in the pristine oceanic atmosphere remains largely unexplored. We used 9 yr of global satellite data and ocean climatologies to derive parameterizations of the temporal variability of (a production fluxes of sulfur aerosols formed by the oxidation of the biogenic gas dimethylsulfide emitted from the sea surface; (b production fluxes of secondary organic aerosols from biogenic organic volatiles; (c emission fluxes of biogenic primary organic aerosols ejected by wind action on sea surface; and (d emission fluxes of sea salt also lifted by the wind upon bubble bursting. Series of global monthly estimates of these fluxes were correlated to series of potential cloud condensation nuclei (CCN numbers derived from satellite (MODIS. More detailed comparisons among weekly series of estimated fluxes and satellite-derived cloud droplet effective radius (re data were conducted at locations spread among polluted and clean regions of the oceanic atmosphere. The outcome of the statistical analysis was that positive correlation to CCN numbers and negative correlation to re were common at mid and high latitude for sulfur and organic secondary aerosols, indicating both might be important in seeding cloud droplet activation. Conversely, primary aerosols (organic and sea salt showed widespread positive correlations to CCN only at low latitudes. Correlations to re were more variable, non-significant or positive, suggesting that, despite contributing to large shares of the marine aerosol mass, primary aerosols are not widespread major drivers of the variability of cloud

  18. Differences of optic disc appearance between normal tension and high tension glaucoma patients

    Directory of Open Access Journals (Sweden)

    Stojčić Milan

    2011-01-01

    Full Text Available Introduction. The term glaucoma is used to cover a wide range of diseases, whose main feature is optic neuropathy. According to the level of intraocular pressure (IOP, the open angle glaucoma is arbitrarily divided into high tension glaucoma (HTG and normal tension glaucoma (NTG. Objective. The aim of this study was to investigate the differences of optic disc cup appearance between patients with NTG and HTG. Methods. Prospective study included 30 patients (60 eyes with NTG and 30 patients (60 eyes with HTG. IOP was measured by Goldmann applanation tonometry. Examination of optic disc head was performed by indirect ophthalmoscopy with Volk 90 D superfield lens through a dilated pupil to observe qualitative and quantitative parameters. Visual fields were examined in all patients with the Octopus program G1, full threshold strategy (Octopus 500 EZ, Interzeag, Switzerland. Results. Vertical form of optic disc cup was present in 11 eyes with NTG (18.3% and three eyes with HTG (5% (p<0.05. A disc with localized tissue loss (polar notching on the inferior pole was observed in eight eyes with NTG (13.3% and in one eye with HTG (1.7% (p<0.01. Uniformly enlarged, round cup was more frequent in patients with HTG than NTG: 93.3% and 68.3% respectively (p<0.05. Conclusion. The perceived differences indicate a complex multifactorial nature of glaucoma disease and a possible existence of two pathophysiological ways of optic disc changes within the same basic disease.

  19. BMI and breast cancer prognosis benefit: mammography screening reveals differences between normal weight and overweight women.

    Science.gov (United States)

    Crispo, Anna; Grimaldi, Maria; D'Aiuto, Massimiliano; Rinaldo, Massimo; Capasso, Immacolata; Amore, Alfonso; D'Aiuto, Giuseppe; Giudice, Aldo; Ciliberto, Gennaro; Montella, Maurizio

    2015-02-01

    Few studies are available on the potential impact of body weight on breast cancer prognosis in screen-detected patients. Moreover, it is not known whether body mass index (BMI) could have a different prognostic impact in screen-detected versus symptomatic breast cancer patients. To investigate these unsolved issues, we carried out a retrospective study evaluating the effect of BMI on breast cancer prognosis in screen-detected vs symptomatic breast cancer patients. We conducted a follow-up study on 448 women diagnosed with incident, histologically-confirmed breast cancer. Patients were categorized according to their BMI as normal weight, overweight and obese. Disease free survival (DFS), overall survival (OS), and BMI curves were compared according to mode of cancer detection. Among screen-detected patients, higher BMI was associated with a significant lower DFS, whereas no significant difference was observed among symptomatic patients. OS showed similar results. In the multivariate analysis adjusting for age, education, tumor size, nodal status, estrogen receptor (ER), progesterone receptor (PR) and menopausal status, the risk for high level of BMI among screen-detected patients did not reach the statistical significance for either recurrence or survival. Our study highlights the potential impact of high bodyweight in breast cancer prognosis, the findings confirm that obesity plays a role in women breast cancer prognosis independently from diagnosis mode. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Impact of different IMRT techniques to improve conformity and normal tissue sparing in upper esophageal cancer

    Directory of Open Access Journals (Sweden)

    Amin E Amin

    2015-03-01

    Full Text Available Purpose: Intensity modulated radiotherapy (IMRT for cervical esophageal cancer is challenging. Although IMRT techniques using inverse planning algorithms are facilitating the treatment planning process, the irradiation dose to the normal tissues can be a critical issue. This study was performed to investigate the effect of beam numbers and their directions and local optimization on: (1 dose conformity and homogeneity to the planning target volume (PTV and (2 dose to the organ at risks (OARs.Methods: Four upper esophageal cancer cases were randomly selected for this treatment planning study. Eight IMRT plans were generated for each case with the same dose-volume constraints but with different beam numbers and arrangements. Local optimization using regular structures drawn automatically around the PTV with margins from 0.5-1.5 cm was performed. IMRT plans were evaluated with respect to isodose distributions, dose-volume histograms (DVHs parameters, homogeneity index (HI, and conformity index (CI. The statistical comparison between the types of plans was done using the One Way ANOVA test.Results: The results showed that IMRT using three or five beams was not sufficient to obtain good dose optimization. The seven field plans showed the best coverage for the PTV with tolerable doses for the OARs, and the beam orientation was very critical. Increasing beams (Bs number from 7 to 13 did not show significant differences in the PTV coverage, while the mean lung dose was increased. The PTV coverage were 95.1, 95.1, 98.1, 97.3, 97.3, 97.3, 97.0, and 97.0% for 3Bs, 5Bs, 7Bs, 9Bs, 13Bs, 7Bs(30, 7Bs(60 (beam angles were changed from 0o to 30o and 60o, and 7Bs(R (seven IMRT plans with ring, respectively. The mean heart dose did not exceed 0.36 Gy with p < 0.05. For lung doses, the best plan was the one with 9Bs which reduced lung volume doses V20Gy (% and V30Gy (%, and reduced mean lung dose from 5.4 to 4.5 Gy with p < 0.05 for 7Bs(R plans. IMRT improved the

  1. High resolution mapping of Normalized Difference Vegetation Indices (NDVI) of biological soil crusts

    Science.gov (United States)

    Fischer, T.; Veste, M.; Eisele, A.; Bens, O.; Spyra, W.; Hüttl, R. F.

    2012-04-01

    Normalized Difference Vegetation Indices (NDVI) are typically determined using satellite or airborne remote sensing, or field portable spectrometers, which give an averaged signal on centimetre to meter scale plots. Biological soil crust (BSC) patches may have smaller sizes, and ecophysiological, hydrological as well as pedological processes may be heterogeneously distributed within this level of resolution. A ground-based NDVI imaging procedure using low-cost equipment (Olympus Camedia 5000z digital camera equipped with a Hoya R72 infrared filter) was developed in this study to fill this gap at the level of field research, where carrying costly and bulky equipment to remote locations is often the limiting factor for data collection. A commercially available colour rendition chart (GretagMacbeth ColorChecker®) with known red (600-700 nm) and NIR (800-900 nm) reflectances was placed into each scene and used for calibration purposes on a per-image basis. Generation of NDVI images involved (i) determination of red and NIR reflectances from the pixel values of the red and NIR channels, respectively, and (ii) calculation and imaging of the NDVI, where NDVI values of -1 to +1 were mapped to grey values of 0 to 255. The correlation between NDVI values retrieved from these images and NDVI values determined using conventional field spectrometry (ASD FieldSpec 3 portable spectroradiometer) was close (r2 =0.91), the 95% confidence interval amounted to 0.10 NDVI units. The pixel resolution was 0.8 mm in the field and 0.2 mm in the laboratory, but can still be improved significantly with closer distance to the crust or with higher camera resolution. Geostatistical analysis revealed that both spatial variability as well as size of individual objects characterized by the NDVI increased with crust development. The latter never exceeded 4 mm in the investigated crusts, which points to the necessity of high resolution imaging for linking remote sensing with ecophysiology

  2. Bond strength of different adhesives to normal and caries-affected dentins

    Institute of Scientific and Technical Information of China (English)

    XUAN Wei; HOU Ben-xiang; L(U) Yalin

    2010-01-01

    Background Currently, several systems of dentin substrate-reacting adhesives are available for use in the restorative treatment against caries. However, the bond effectiveness and property of different adhesive systems to caries-affected dentin are not fully understood. The objective of this study was to evaluate the bond strength of different adhesives to both normal dentin (ND) and caries-affected dentin (CAD) and to analyze the dentin/adhesive interracial characteristics.Methods Twenty eight extracted human molars with coronal medium carious lesions were randomly assigned to four groups according to adhesives used. ND and CAD were bonded with etch-and-rinse adhesive Adper~(TM) Single Bond 2 (SB2) or self-etching adhesives Clearfil SE Bond (CSE), Clearfil S~3 Bond (CS3), iBond GI (IB). Rectangular sticks of resin-dentin bonded interfaces 0.9 mm~2 were obtained. The specimens were subjected to microtensile bond strength (μTBS) testing at a crosshead speed of 1 mm/min. Mean μTBS was statistically analyzed with analysis of variance (ANOVA) and Student-Newman-Keuls tests. Interfacial morphologies were analyzed by Scanning Electron Microscopy (SEM).Results Etch-and-rinse adhesive Adper~(TM) Single Bond 2 yielded high bond strength when applied to both normal and caries-affected dentin. The two-step self-etching adhesive Clearfil SE Bond generated the highest bond strength to ND among all adhesives tested but a significantly reduced strength when applied to CAD. For the one-step self-etching adhesives, Clearfil S~3 Bond and iBond GI, the bond strength was relatively low regardless of the dentin type. SEM interfacial analysis revealed that hybrid layers were thicker with poorer resin tag formation and less resin-filled lateral branches in the CAD than in the ND for all the adhesives tested.Conclusion The etch-and-rinse adhesive performed more effectively to caries-affected dentin than the self-etching adhesives.

  3. Sampling errors for satellite-derived tropical rainfall - Monte Carlo study using a space-time stochastic model

    Science.gov (United States)

    Bell, Thomas L.; Abdullah, A.; Martin, Russell L.; North, Gerald R.

    1990-01-01

    Estimates of monthly average rainfall based on satellite observations from a low earth orbit will differ from the true monthly average because the satellite observes a given area only intermittently. This sampling error inherent in satellite monitoring of rainfall would occur even if the satellite instruments could measure rainfall perfectly. The size of this error is estimated for a satellite system being studied at NASA, the Tropical Rainfall Measuring Mission (TRMM). First, the statistical description of rainfall on scales from 1 to 1000 km is examined in detail, based on rainfall data from the Global Atmospheric Research Project Atlantic Tropical Experiment (GATE). A TRMM-like satellite is flown over a two-dimensional time-evolving simulation of rainfall using a stochastic model with statistics tuned to agree with GATE statistics. The distribution of sampling errors found from many months of simulated observations is found to be nearly normal, even though the distribution of area-averaged rainfall is far from normal. For a range of orbits likely to be employed in TRMM, sampling error is found to be less than 10 percent of the mean for rainfall averaged over a 500 x 500 sq km area.

  4. Normal inter-limb differences during the straight leg raise neurodynamic test: a cross sectional study

    Directory of Open Access Journals (Sweden)

    Boyd Benjamin S

    2012-12-01

    Full Text Available Abstract Background The straight leg raise (SLR neurodynamic test is commonly used to examine the sensitivity of the lower quarter nervous system to movement. Range of motion during the SLR varies considerably, due to factors such as age, sex and activity level. Knowing intra-individual, inter-limb differences may provide a normative measure that is not influenced by such demographic characteristics. This study aimed to determine normal asymmetries between limbs in healthy, asymptomatic individuals during SLR testing and the relationship of various demographic characteristics. Methods The limb elevation angle was measured using an inclinometer during SLR neurodynamic testing that involved pre-positioning the ankle in plantar flexion (PF/SLR and neutral dorsiflexion (DF/SLR. Phase 1 of the study included 20 participants where the ankle was positioned using an ankle brace replicating research testing conditions. Phase 2 included 20 additional participants where the ankle was manually positioned to replicate clinical testing conditions. Results The group average range of motion during PF/SLR was 57.1 degrees (SD: 16.8 degrees on the left and 56.7 degrees (SD: 17.2 degrees on the right while during DF/SLR the group average was 48.5 degrees (SD: 16.1 degrees on the left and 48.9 degrees (SD: 16.4 degrees on the right. The range of motion during SLR was moderately correlated to weight (−0.40 to −0.52, body mass index (−0.41 to −0.52, sex (0.40 to 0.42 and self-reported activity level (0.50 to 0.57. Intra-individual differences between limbs for range of motion during PF/SLR averaged 5.0 degrees (SD: 3.5 degrees (95% CI: 3.8 degrees, 6.1 degrees and during DF/SLR averaged 4.1 degrees (SD: 3.2 degrees (95% CI: 3.1 degrees, 5.1 degrees but were not correlated with any demographic characteristic. There were no significant differences between Phase 1 and Phase 2. Conclusions Overall range of motion during SLR was related to sex, weight, BMI and

  5. The Effects of Different Doses of Ketamine on Quality of Normal Ejaculated Sperm

    Directory of Open Access Journals (Sweden)

    Forouzan Absalan

    2014-07-01

    Full Text Available Background: Ketamine, an injectable anesthetic in human and animal medicine, is also a recreational drug used by young adults. The aim of this study is to evaluate the effects of ketamine on membrane integrity, DNA fragmentation and sperm parameters in humans. Materials and Methods: This prospective study was conducted on 40 males with normal semen samples over one month (August 2012. Subjects were randomly allocated to four groups (Control and case I, II and III whose semen samples were adjusted to different concentrations of ketamine (1, 3, 5 μL for one hour. Sperm analysis was performed for routine parameters, motility and morphology. Evaluation of membrane integrity and DNA fragmentation was done by eosin-Y staining and the sperm chromatin dispersion (SCD test, respectively. The results were analyzed by ANOVA and Tukey’s tests. P≤0.05 was considered statistically significant. Results: Total sperm motility in all case groups were significantly lower compared with the control group. In case group III, progressive motility showed significant difference with case group II. After addition of ketamine, sperm had evidence of coiled tails in all case groups compared to the control group however this observation was not significant. Evaluation of membrane integrity showed the rate of necrospermia increased in all case groups. However, ketamine only significantly affected membrane integrity in case group III. SCD staining showed that in the control group nucleoids with medium halos (63.44 ± 1.2 were significantly different compared to the case groups I (15.44 ± 0.45, II (9.05±1.16 and III (10.55 ± 1.14, respectively. Between case groups, nucleoids with large and medium halos showed significant differences in case groups II and III compared with case group I. Nucleoids with medium halos were significantly different between case groups II and III. Conclusion: Ketamine, through its effect on membrane integrity and DNA fragmentation, decreased

  6. Prognostic land surface albedo from a dynamic global vegetation model clumped canopy radiative transfer scheme and satellite-derived geographic forest heights

    Science.gov (United States)

    Kiang, N. Y.; Yang, W.; Ni-Meister, W.; Aleinov, I. D.; Jonas, J.

    2014-12-01

    Vegetation cover was introduced into general circulations models (GCMs) in the 1980's to account for the effect of land surface albedo and water vapor conductance on the Earth's climate. Schemes assigning canopy albedoes by broad biome type have been superceded in 1990's by canopy radiative transfer schemes for homogeneous canopies obeying Beer's Law extinction as a function of leaf area index (LAI). Leaf albedo and often canopy height are prescribed by plant functional type (PFT). It is recognized that this approach does not effectively describe geographic variation in the radiative transfer of vegetated cover, particularly for mixed and sparse canopies. GCM-coupled dynamic global vegetation models (DGVMs) have retained these simple canopy representations, with little further evaluation of their albedos. With the emergence lidar-derived canopy vertical structure data, DGVM modelers are now revisiting albedo simulation. We present preliminary prognostic global land surface albedo produced by the Ent Terrestrial Biosphere Model (TBM), a DGVM coupled to the NASA Goddard Institute for Space Studies (GISS) GCM. The Ent TBM is a next generation DGVM designed to incorporate variation in canopy heights, and mixed and sparse canopies. For such dynamically varying canopy structure, it uses the Analytical Clumped Two-Stream (ACTS) canopy radiative transfer model, which is derived from gap probability theory for canopies of tree cohorts with ellipsoidal crowns, and accounts for soil, snow, and bare stems. We have developed a first-order global vegetation structure data set (GVSD), which gives a year of satellite-derived geographic variation in canopy height, maximum canopy leaf area, and seasonal LAI. Combined with Ent allometric relations, this data set provides population density and foliage clumping within crowns. We compare the Ent prognostic albedoes to those of the previous GISS GCM scheme, and to satellite estimates. The impact of albedo differences on surface

  7. Exosomes from Osteosarcoma and normal osteoblast differ in proteomic cargo and immunomodulatory effects on T cells.

    Science.gov (United States)

    Troyer, Ryan M; Ruby, Carl E; Goodall, Cheri P; Yang, Liping; Maier, Claudia S; Albarqi, Hassan A; Brady, Jacqueline V; Bathke, Kallan; Taratula, Oleh; Mourich, Dan; Bracha, Shay

    2017-09-15

    Canine osteosarcoma (OSA) is the most common cancer of the appendicular skeleton and is associated with high metastatic rate to the lungs and poor prognosis. Recent studies have shown the impact of malignant-derived exosomes on immune cells and the facilitation of immune evasion. In the current study, we have characterized the proteomic profile of exosomes derived from healthy osteoblasts and osteosarcoma cell lines. We investigated the direct impact of these exosomes on healthy T cells. Proteomic cargo of the malignant exosomes was markedly different from osteoblastic exosomes and contained immunosuppressive proteins including TGF-β, α fetoprotein and heat shock proteins. OSA exosomes directly attenuated the rate of T cell proliferation, increased a regulatory (FoxP3+) CD4+ phenotype and diminished the expression of the activation marker CD25+ on CD8+ cells. Exosomes of osteoblasts also demonstrated a direct impact on T cells, but to a lesser degree. Osteosarcoma-derived exosomes compared to normal osteoblasts contain an immunomodulatory cargo, which reduced the rate of T cell proliferation and promoted T regulatory phenotype. Osteoblast-derived exosomes can also reduce T cell activity, but to lesser degree compared to OSA exosomes and without promoting a T regulatory phenotype. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Haze detection by using modified normalized difference haze index in Beijing, Tianjin, and Hebei province

    Science.gov (United States)

    Han, Xinlei; Yao, Fengmei; Zhang, Jiahua; Waqar, Mirza Muhammad; Zha, Yong; He, Junliang

    2016-04-01

    This paper presents the development of index to detect haze from moderate resolution imaging spectroradiometer remote sensing data. Detection of haze over a large area has always been a problem. This study focuses on Beijing, Tianjin, and Shijiazhuang cities in China. These cities have suffered the worst hazy weather in recent years. The spectral influence of haze on surface features was determined through analysis of the spectral variations of surface covers between hazy and haze-free days. A spectral index known as modified normalized difference haze index (m-NDHI) is developed that can be used to monitor haze distribution and intensity. Correlation analysis of the derived m-NDHI and previously developed NDHI with in situ PM2.5 (particulate matter with diameter <2.5 μm) data reveals that m-NDHI over water bodies has a coefficient of 0.7096, 0.5864, and 0.4857 and NDHI has coefficient of 0.5625, 0.5321, and 0.4618 with PM2.5 for Beijing, Tianjin, and Shijiazhuang, respectively, in winter. Moreover, the correlation of m-NDHI with PM2.5 is 0.4097, 0.8092, and 0.5546 during the spring, summer, and autumn, respectively, in Beijing. This developed index can be a much easier and more effective method to detect haze in large scales from remotely sensing data and characterize the situation of urban atmospheric pollution.

  9. Estimating Sugarcane Yield Potential Using an In-Season Determination of Normalized Difference Vegetative Index

    Directory of Open Access Journals (Sweden)

    Howard Viator

    2012-06-01

    Full Text Available Estimating crop yield using remote sensing techniques has proven to be successful. However, sugarcane possesses unique characteristics; such as, a multi-year cropping cycle and plant height-limiting for midseason fertilizer application timing. Our study objective was to determine if sugarcane yield potential could be estimated using an in-season estimation of normalized difference vegetative index (NDVI. Sensor readings were taken using the GreenSeeker® handheld sensor from 2008 to 2011 in St. Gabriel and Jeanerette, LA, USA. In-season estimates of yield (INSEY values were calculated by dividing NDVI by thermal variables. Optimum timing for estimating sugarcane yield was between 601–750 GDD. In-season estimated yield values improved the yield potential (YP model compared to using NDVI. Generally, INSEY value showed a positive exponential relationship with yield (r2 values 0.48 and 0.42 for cane tonnage and sugar yield, respectively. When models were separated based on canopy structure there was an increase the strength of the relationship for the erectophile varieties (r2 0.53 and 0.47 for cane tonnage and sugar yield, respectively; however, the model for planophile varieties weakened slightly. Results of this study indicate using an INSEY value for predicting sugarcane yield shows potential of being a valuable management tool for sugarcane producers in Louisiana.

  10. Estimating sugarcane yield potential using an in-season determination of normalized difference vegetative index.

    Science.gov (United States)

    Lofton, Josh; Tubana, Brenda S; Kanke, Yumiko; Teboh, Jasper; Viator, Howard; Dalen, Marilyn

    2012-01-01

    Estimating crop yield using remote sensing techniques has proven to be successful. However, sugarcane possesses unique characteristics; such as, a multi-year cropping cycle and plant height-limiting for midseason fertilizer application timing. Our study objective was to determine if sugarcane yield potential could be estimated using an in-season estimation of normalized difference vegetative index (NDVI). Sensor readings were taken using the GreenSeeker® handheld sensor from 2008 to 2011 in St. Gabriel and Jeanerette, LA, USA. In-season estimates of yield (INSEY) values were calculated by dividing NDVI by thermal variables. Optimum timing for estimating sugarcane yield was between 601-750 GDD. In-season estimated yield values improved the yield potential (YP) model compared to using NDVI. Generally, INSEY value showed a positive exponential relationship with yield (r(2) values 0.48 and 0.42 for cane tonnage and sugar yield, respectively). When models were separated based on canopy structure there was an increase the strength of the relationship for the erectophile varieties (r(2) 0.53 and 0.47 for cane tonnage and sugar yield, respectively); however, the model for planophile varieties weakened slightly. Results of this study indicate using an INSEY value for predicting sugarcane yield shows potential of being a valuable management tool for sugarcane producers in Louisiana.

  11. Protecting contacts of hepatitis A: what's the difference between vaccine and human normal immunoglobulin?

    Science.gov (United States)

    Crowcroft, N S

    2008-01-01

    The efficacy of vaccine when time since exposure is prolonged (more than 1 week from onset of illness in the index case) is unknown, but is likely to be significantly lower than human normal immunoglobulin (HNIG). We estimated the number of additional secondary cases that may occur through giving vaccine instead of HNIG to contacts of cases of hepatitis A who are identified more than 1 week after onset in the index case. This was calculated for different levels of vaccine efficacy, assuming HNIG efficacy to be 80-90%. The number of households that need to be treated to prevent one secondary case was calculated using estimates of secondary attack ratios (AR). If more than 1 week has elapsed from onset of illness in the index case, for an average household size of 2.3 people, a vaccine efficacy of 50% and an AR of 10-25%, 8-26 households would need to be treated with vaccine before one additional secondary case would be observed. As UK public health professionals manage around one hepatitis A case per month, it would take from 8 months to over 2 years for them to observe one additional case amongst contacts using vaccine rather than HNIG. It is unlikely that an average practitioner would notice if vaccine were 30% less effective than HNIG. Public health practice and advice to patients and contacts should be based on evidence as well as experience.

  12. Dopamine transporter availability in clinically normal aging is associated with individual differences in white matter integrity

    Science.gov (United States)

    Rieckmann, Anna; Hedden, Trey; Younger, Alayna P.; Sperling, Reisa A.; Johnson, Keith A.; Buckner, Randy L.

    2016-01-01

    Aging-related differences in white matter integrity, the presence of amyloid plaques, and density of biomarkers indicative of dopamine functions can be detected and quantified with in vivo human imaging. The primary aim of the present study was to investigate whether these imaging-based measures constitute independent imaging biomarkers in older adults, which would speak to the hypothesis that the aging brain is characterized by multiple independent neurobiological cascades. We assessed MRI-based markers of white matter integrity and PET-based marker of dopamine transporter density and amyloid deposition in the same set of 53 clinically normal individuals (age 65–87). A multiple regression analysis demonstrated that dopamine transporter availability is predicted by white matter integrity, which was detectable even after controlling for chronological age. Further post-hoc exploration revealed that dopamine transporter availability was further associated with systolic blood pressure, mirroring the established association between cardiovascular health and white matter integrity. Dopamine transporter availability was not associated with the presence of amyloid burden. Neurobiological correlates of dopamine transporter measures in aging are therefore likely unrelated to Alzheimer’s disease but are aligned with white matter integrity and cardiovascular risk. More generally, these results suggest that two common imaging markers of the aging brain that are typically investigated separately do not reflect independent neurobiological processes. PMID:26542307

  13. Sugarcane leaf area estimate obtained from the corrected Normalized Difference Vegetation Index (NDVI

    Directory of Open Access Journals (Sweden)

    Rodrigo Moura Pereira

    2016-06-01

    Full Text Available Large farmland areas and the knowledge on the interaction between solar radiation and vegetation canopies have increased the use of data from orbital remote sensors in sugarcane monitoring. However, the constituents of the atmosphere affect the reflectance values obtained by imaging sensors. This study aimed at improving a sugarcane Leaf Area Index (LAI estimation model, concerning the Normalized Difference Vegetation Index (NDVI subjected to atmospheric correction. The model generated by the NDVI with atmospheric correction showed the best results (R2 = 0.84; d = 0.95; MAE = 0.44; RMSE = 0.55, in relation to the other models compared. LAI estimation with this model, during the sugarcane plant cycle, reached a maximum of 4.8 at the vegetative growth phase and 2.3 at the end of the maturation phase. Thus, the use of atmospheric correction to estimate the sugarcane LAI is recommended, since this procedure increases the correlations between the LAI estimated by image and by plant parameters.

  14. Global Trends in Seasonality of Normalized Difference Vegetation Index (NDVI, 1982–2011

    Directory of Open Access Journals (Sweden)

    Assaf Anyamba

    2013-09-01

    Full Text Available A 30-year series of global monthly Normalized Difference Vegetation Index (NDVI imagery derived from the Global Inventory Modeling and Mapping Studies (GIMMS NDVI3g archive was analyzed for the presence of trends in changing seasonality. Using the Seasonal Trend Analysis (STA procedure, over half (56.30% of land surfaces were found to exhibit significant trends. Almost half (46.10% of the significant trends belonged to three classes of seasonal trends (or changes. Class 1 consisted of areas that experienced a uniform increase in NDVI throughout the year, and was primarily associated with forested areas, particularly broadleaf forests. Class 2 consisted of areas experiencing an increase in the amplitude of the annual seasonal signal whereby increases in NDVI in the green season were balanced by decreases in the brown season. These areas were found primarily in grassland and shrubland regions. Class 3 was found primarily in the Taiga and Tundra biomes and exhibited increases in the annual summer peak in NDVI. While no single attribution of cause could be determined for each of these classes, it was evident that they are primarily found in natural areas (as opposed to anthropogenic land cover conversions and that they are consistent with climate-related ameliorations of growing conditions during the study period.

  15. Protoporphyrin IX formation and photobleaching in different layers of normal human skin

    DEFF Research Database (Denmark)

    Togsverd-Bo, Katrine; Idorn, Luise W; Philipsen, Peter A

    2012-01-01

    human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18......Topical photodynamic therapy (PDT) is used for various skin disorders, and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal...... and 37 J/cm(2)), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm(2)) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm(2)) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than...

  16. Dopamine transporter availability in clinically normal aging is associated with individual differences in white matter integrity.

    Science.gov (United States)

    Rieckmann, Anna; Hedden, Trey; Younger, Alayna P; Sperling, Reisa A; Johnson, Keith A; Buckner, Randy L

    2016-02-01

    Aging-related differences in white matter integrity, the presence of amyloid plaques, and density of biomarkers indicative of dopamine functions can be detected and quantified with in vivo human imaging. The primary aim of the present study was to investigate whether these imaging-based measures constitute independent imaging biomarkers in older adults, which would speak to the hypothesis that the aging brain is characterized by multiple independent neurobiological cascades. We assessed MRI-based markers of white matter integrity and PET-based marker of dopamine transporter density and amyloid deposition in the same set of 53 clinically normal individuals (age 65-87). A multiple regression analysis demonstrated that dopamine transporter availability is predicted by white matter integrity, which was detectable even after controlling for chronological age. Further post-hoc exploration revealed that dopamine transporter availability was further associated with systolic blood pressure, mirroring the established association between cardiovascular health and white matter integrity. Dopamine transporter availability was not associated with the presence of amyloid burden. Neurobiological correlates of dopamine transporter measures in aging are therefore likely unrelated to Alzheimer's disease but are aligned with white matter integrity and cardiovascular risk. More generally, these results suggest that two common imaging markers of the aging brain that are typically investigated separately do not reflect independent neurobiological processes. Hum Brain Mapp 37:621-631, 2016. © 2015 Wiley Periodicals, Inc.

  17. Grouping Normal Type Ia Supernovae by UV to Optical Color Differences

    CERN Document Server

    Milne, Peter A; Roming, Peter W A; Bufano, Filomena; Gehrels, Neil

    2013-01-01

    Observations of many SNe Ia with the UVOT instrument on the Swift satellite has revealed that there exists order to the differences in the UV-OPT colors of normal SNe. We examine UV-OPT color curves for 25 SNe Ia, dividing them into 4 groups, finding that ~1/3 of these SNe Ia have bluer UV-OPT colors than the larger group, with these "NUV-blue" SNe Ia 0.4 mag bluer than the "NUV-red" SNe Ia in u-v. Another group of events feature colors similar to NUV-red SNe Ia in the u-v to uvw1-v colors, but similar to the NUV-blue SNe Ia in the uvm2-v color. We name these events "MUV-blue". The last group initially has colors similar to NUV-red SNe Ia, but with color curves that feature more modest changes than the larger NUV-red group. These "irregular" events are comprised of all the NUV-red events with the broadest optical peaks, which leads us to consider this minor group a subset of the NUV-red group. When so separated and the accounting is made for the rapid time evolution of the UV-OPT colors, we find that the scat...

  18. Spatiotemporal variability and predictability of Normalized Difference Vegetation Index (NDVI) in Alberta, Canada

    Science.gov (United States)

    Jiang, Rengui; Xie, Jiancang; He, Hailong; Kuo, Chun-Chao; Zhu, Jiwei; Yang, Mingxiang

    2016-09-01

    As one of the most popular vegetation indices to monitor terrestrial vegetation productivity, Normalized Difference Vegetation Index (NDVI) has been widely used to study the plant growth and vegetation productivity around the world, especially the dynamic response of vegetation to climate change in terms of precipitation and temperature. Alberta is the most important agricultural and forestry province and with the best climatic observation systems in Canada. However, few studies pertaining to climate change and vegetation productivity are found. The objectives of this paper therefore were to better understand impacts of climate change on vegetation productivity in Alberta using the NDVI and provide reference for policy makers and stakeholders. We investigated the following: (1) the variations of Alberta's smoothed NDVI (sNDVI, eliminated noise compared to NDVI) and two climatic variables (precipitation and temperature) using non-parametric Mann-Kendall monotonic test and Thiel-Sen's slope; (2) the relationships between sNDVI and climatic variables, and the potential predictability of sNDVI using climatic variables as predictors based on two predicted models; and (3) the use of a linear regression model and an artificial neural network calibrated by the genetic algorithm (ANN-GA) to estimate Alberta's sNDVI using precipitation and temperature as predictors. The results showed that (1) the monthly sNDVI has increased during the past 30 years and a lengthened growing season was detected; (2) vegetation productivity in northern Alberta was mainly temperature driven and the vegetation in southern Alberta was predominantly precipitation driven for the period of 1982-2011; and (3) better performances of the sNDVI-climate relationships were obtained by nonlinear model (ANN-GA) than using linear (regression) model. Similar results detected in both monthly and summer sNDVI prediction using climatic variables as predictors revealed the applicability of two models for

  19. Effectiveness of Three Different Walking Prescription Durations on Total Physical Activity in Normal- and Overweight Women

    Directory of Open Access Journals (Sweden)

    Yves Schutz

    2014-08-01

    Full Text Available Objective: While there is a dose-response relationship between physical activity (PA and health benefit, little is known about the effectiveness of different PA prescriptions on total daily PA. Aim: To test, under real-life conditions and using an objective, non-invasive measurement technique (accelerometry, the effect of prescribing additional physical activity (walking only of different durations (30, 60 and 90 min/day on compliance (to the activity prescribed and compensation (to total daily PA. Participants in each group were prescribed 5 sessions of walking per week over 4 weeks. Methods: 55 normal-weight and overweight women (mean BMI 25 ± 5 kg/m2, height 165 ± 1 cm, weight 68 ± 2 kg and mean age 27 ± 1 years were randomly assigned to 3 prescription groups: 30, 60 or 90 min/day PA. Results: Walking duration resulted in an almost linear increase in the number of steps per day during the prescription period from an average of about 10,000 steps per day for the 30-min prescription to about 14,000 for the 90-min prescription. Compliance was excellent for the 30-min prescription but decreased significantly with 60-min and 90-min prescriptions. In parallel, degree of compensation subsequent to exercise increased progressively as length of prescription increased. Conclusion: A 30-min prescription of extra walking 5 times per week was well tolerated. However, in order to increase total PA further, much more than 60 min of walking may need to be prescribed in the majority of individuals. While total exercise ‘volume' increased with prescriptions longer than 30 min, compliance to the prescription decreased and greater compensation was evident.

  20. Evaluation of vegetation cover using the normalized difference vegetation index (NDVI

    Directory of Open Access Journals (Sweden)

    Gabriela Camargos Lima

    2013-08-01

    Full Text Available Soil loss by water erosion is the main cause of soil degradation in Brazil. However, erosion can be reduced by the presence of vegetation. The Normalized Difference Vegetation Index (NDVI makes it possible to identify the vegetative vigor of crops or natural vegetation which facilities the identification of areas with vegetation covers. This information is very important in identifying the phenomena which might be occurring in a particular area, especially those related to soil degradation by water erosion. Thus, the aim of this work was to assess the canopy cover by using NDVI, checking the image accuracy using the Coverage Index (CI based on the Stocking method, in the Sub-basin of Posses, which belongs to the Cantareira System, located in the Extrema municipality, Minas Gerais, Brazil. Landsat-5 TM images were used. The sub-basin of Posses was very altered in comparison to the surrounding areas. The NDVI technique proved to be a suitable tool to assess the uses that occur in the sub-basin of Posses, as validated by the Stocking methodology. The map derived from NDVI allowed the geographic distribution of different land uses to be observed and allowed for the identification of critical areas in relation to vegetation cover as well. This finding can be used to optimize efforts to recover and protect soil in areas with bare soil and degraded pasture, in order to reduce environmental degradation. The CI has not exceeded 40% for land use classes that occur in the majority of the sub-basin (91%, except in areas of woody vegetation.

  1. Spatiotemporal variability and predictability of Normalized Difference Vegetation Index (NDVI) in Alberta, Canada.

    Science.gov (United States)

    Jiang, Rengui; Xie, Jiancang; He, Hailong; Kuo, Chun-Chao; Zhu, Jiwei; Yang, Mingxiang

    2016-09-01

    As one of the most popular vegetation indices to monitor terrestrial vegetation productivity, Normalized Difference Vegetation Index (NDVI) has been widely used to study the plant growth and vegetation productivity around the world, especially the dynamic response of vegetation to climate change in terms of precipitation and temperature. Alberta is the most important agricultural and forestry province and with the best climatic observation systems in Canada. However, few studies pertaining to climate change and vegetation productivity are found. The objectives of this paper therefore were to better understand impacts of climate change on vegetation productivity in Alberta using the NDVI and provide reference for policy makers and stakeholders. We investigated the following: (1) the variations of Alberta's smoothed NDVI (sNDVI, eliminated noise compared to NDVI) and two climatic variables (precipitation and temperature) using non-parametric Mann-Kendall monotonic test and Thiel-Sen's slope; (2) the relationships between sNDVI and climatic variables, and the potential predictability of sNDVI using climatic variables as predictors based on two predicted models; and (3) the use of a linear regression model and an artificial neural network calibrated by the genetic algorithm (ANN-GA) to estimate Alberta's sNDVI using precipitation and temperature as predictors. The results showed that (1) the monthly sNDVI has increased during the past 30 years and a lengthened growing season was detected; (2) vegetation productivity in northern Alberta was mainly temperature driven and the vegetation in southern Alberta was predominantly precipitation driven for the period of 1982-2011; and (3) better performances of the sNDVI-climate relationships were obtained by nonlinear model (ANN-GA) than using linear (regression) model. Similar results detected in both monthly and summer sNDVI prediction using climatic variables as predictors revealed the applicability of two models for

  2. Using normalized difference vegetation index to estimate carbon fluxes from small rotationally grazed pastures

    Science.gov (United States)

    Skinner, R.H.; Wylie, B.K.; Gilmanov, T.G.

    2011-01-01

    Satellite-based normalized difference vegetation index (NDVI) data have been extensively used for estimating gross primary productivity (GPP) and yield of grazing lands throughout the world. However, the usefulness of satellite-based images for monitoring rotationally-grazed pastures in the northeastern United States might be limited because paddock size is often smaller than the resolution limits of the satellite image. This research compared NDVI data from satellites with data obtained using a ground-based system capable of fine-scale (submeter) NDVI measurements. Gross primary productivity was measured by eddy covariance on two pastures in central Pennsylvania from 2003 to 2008. Weekly 250-m resolution satellite NDVI estimates were also obtained for each pasture from the moderate resolution imaging spectroradiometer (MODIS) sensor. Ground-based NDVI data were periodically collected in 2006, 2007, and 2008 from one of the two pastures. Multiple-regression and regression-tree estimates of GPP, based primarily on MODIS 7-d NDVI and on-site measurements of photosynthetically active radiation (PAR), were generally able to predict growing-season GPP to within an average of 3% of measured values. The exception was drought years when estimated and measured GPP differed from each other by 11 to 13%. Ground-based measurements improved the ability of vegetation indices to capture short-term grazing management effects on GPP. However, the eMODIS product appeared to be adequate for regional GPP estimates where total growing-season GPP across a wide area would be of greater interest than short-term management-induced changes in GPP at individual sites.

  3. Difference in gene expression of macrophage between normal spleen and portal hypertensive spleen idendified by cDNA microarray

    OpenAIRE

    2007-01-01

    AIM: To identify the difference in gene expression of microphage (Mφ) between normal spleen and portal hypertensive spleen using cDNA microarrays and find new gene functions associated with hypersplenism in portal hypertension.

  4. The Application of Normal Stress Reduction Function in Tilt Tests for Different Block Shapes

    Science.gov (United States)

    Kim, Dong Hyun; Gratchev, Ivan; Hein, Maw; Balasubramaniam, Arumugam

    2016-08-01

    This paper focuses on the influence of the shapes of rock cores, which control the sliding or toppling behaviours in tilt tests for the estimation of rock joint roughness coefficients (JRC). When the JRC values are estimated by performing tilt tests, the values are directly proportional to the basic friction of the rock material and the applied normal stress on the sliding planes. Normal stress obviously varies with the shape of the sliding block, and the basic friction angle is also affected by the sample shapes in tilt tests. In this study, the shapes of core blocks are classified into three representative shapes and those are created using plaster. Using the various shaped artificial cores, a set of tilt tests is carried out to identify the shape influences on the normal stress and the basic friction angle in tilt tests. The test results propose a normal stress reduction function to estimate the normal stress for tilt tests according to the sample shapes based on Barton's empirical equation. The proposed normal stress reduction functions are verified by tilt tests using artificial plaster joints and real rock joint sets. The plaster joint sets are well matched and cast in detailed printed moulds using a 3D printing technique. With the application of the functions, the obtained JRC values from the tilt tests using the plaster samples and the natural rock samples are distributed within a reasonable JRC range when compared with the measured values.

  5. Trends in normalized difference vegetation index (NDVI) associated with urban development in northern West Siberia

    Science.gov (United States)

    Esau, Igor; Miles, Victoria V.; Davy, Richard; Miles, Martin W.; Kurchatova, Anna

    2016-08-01

    Exploration and exploitation of oil and gas reserves of northern West Siberia has promoted rapid industrialization and urban development in the region. This development leaves significant footprints on the sensitive northern environment, which is already stressed by the global warming. This study reports the region-wide changes in the vegetation cover as well as the corresponding changes in and around 28 selected urbanized areas. The study utilizes the normalized difference vegetation index (NDVI) from high-resolution (250 m) MODIS data acquired for summer months (June through August) over 15 years (2000-2014). The results reveal the increase of NDVI (or "greening") over the northern (tundra and tundra-forest) part of the region. Simultaneously, the southern, forested part shows the widespread decrease of NDVI (or "browning"). These region-wide patterns are, however, highly fragmented. The statistically significant NDVI trends occupy only a small fraction of the region. Urbanization destroys the vegetation cover within the developed areas and at about 5-10 km distance around them. The studied urbanized areas have the NDVI values by 15 to 45 % lower than the corresponding areas at 20-40 km distance. The largest NDVI reduction is typical for the newly developed areas, whereas the older areas show recovery of the vegetation cover. The study reveals a robust indication of the accelerated greening near the older urban areas. Many Siberian cities become greener even against the wider browning trends at their background. Literature discussion suggests that the observed urban greening could be associated not only with special tending of the within-city green areas but also with the urban heat islands and succession of more productive shrub and tree species growing on warmer sandy soils.

  6. Exploratory normalized difference water indices for semi-automated extraction of Antarctic lake features

    Science.gov (United States)

    Jawak, Shridhar D.; Luis, Alvarinho J.

    2016-05-01

    This work presents various normalized difference water indices (NDWI) to delineate lakes from Schirmacher Oasis, East Antarctica, by using a very high resolution WorldView-2 (WV-2) satellite imagery. Schirmacher oasis region hosts a number of fresh as well as saline water lakes, such as epishelf lakes, ice-free or landlocked lakes, which are completely frozen or semi-frozen and in a ice-free state. Hence, detecting all these types of lakes distinctly on satellite imagery was the major challenge, as the spectral characteristics of various types of lakes were identical to the other land cover targets. Multiband spectral index pixel-based approach is most experimented and recently growing technique because of its unbeatable advantages such as its simplicity and comparatively lesser amount of processing-time. In present study, semiautomatic extraction of lakes in cryospheric region was carried out by designing specific spectral indices. The study utilized number of existing spectral indices to extract lakes but none could deliver satisfactory results and hence we modified NDWI. The potentials of newly added bands in WV-2 satellite imagery was explored by developing spectral indices comprising of Yellow (585 - 625 nm) band, in combination with Blue (450 - 510 nm), Coastal (400 - 450 nm) and Green (510 - 580 nm) bands. For extraction of frozen lakes, use of Yellow (585 - 625 nm) and near-infrared 2 (NIR2) band pair, and Yellow and Green band pair worked well, whereas for ice-free lakes extraction, a combination of Blue and Coastal band yielded appreciable results, when compared with manually digitized data. The results suggest that the modified NDWI approach rendered bias error varying from 1 to 34 m2.

  7. Effect of different protein types on second meal postprandial glycaemia in normal weight and normoglycemic subjects

    Directory of Open Access Journals (Sweden)

    Winder Tadeu Silva Ton

    2014-03-01

    Full Text Available Background: Diabetes mellitus is a global epidemic affecting 346 million people in the world. The glycemic control is the key for diabetes prevention and management. Some proteins can stimulate insulin release and modulate glycemic response. Objectives: To assess the effect of the consumption of different types of protein (whey protein, soy protein and egg white on a second meal postprandial glycaemia in normal weight and normoglycemic subjects. Methodology: Randomized crossover clinical trial. After an overnight fast of 12-hours, ten subjects attended the laboratory to drink one of the protein shakes (whey, soy or egg white or the control drink. Thirty minutes later, the subjects consumed a glucose solution (25 g glucose. Glycemic response was monitored at times 0 (before glucose solution and 15, 30, 45, 60, 90 and 120 min (after glucose solution consumption. Incremental area under the glycemic curve (iAUC was calculated by the trapezoidal method. Furthermore, glycemic response was assessed by a new method using iG equation. Results: Compared with control, whey and soy protein drinks reduced postprandial iAUC in 56.5% (p = 0.004 and 44.4% (p = 0.029, respectively. Whey protein was the only protein capable of avoiding great fluctuations and a peak in postprandial glycemia. The assessment of glycemic response by iG equation showed positive correlation with iAUC (Pearson 0.985, p < 0.05. Conclusion: The consumption of whey and soy protein 30 minutes before a glucose load resulted in lower iAUC compared with control drink. Whey protein maintained postprandial glycemia more stable.

  8. Protoporphyrin IX formation and photobleaching in different layers of normal human skin: methyl- and hexylaminolevulinate and different light sources.

    Science.gov (United States)

    Togsverd-Bo, Katrine; Idorn, Luise W; Philipsen, Peter A; Wulf, Hans Christian; Hædersdal, Merete

    2012-10-01

    Topical photodynamic therapy (PDT) is used for various skin disorders, and selective targeting of specific skin structures is desirable. The objective was to assess accumulation of PpIX fluorescence and photobleaching within skin layers using different photosensitizers and light sources. Normal human skin was tape-stripped and incubated with 20% methylaminolevulinate (MAL) or 20% hexylaminolevulinate (HAL) for 3 h. Fluorescence microscopy quantified PpIX accumulation in epidermis, superficial, mid and deep dermis, down to 2 mm. PpIX photobleaching by light-emitting diode (LED, 632 nm, 18 and 37 J/cm(2)), intense pulsed light (IPL, 500-650 nm, 36 and 72 J/cm(2)) and long-pulsed dye laser (LPDL, 595 nm, 7.5 and 15 J/cm(2)) was measured using fluorescence photography and microscopy. We found higher PpIX fluorescence intensities in epidermis and superficial dermis in HAL-incubated skin than MAL-incubated skin (P dermis, fluorescence intensities were higher (37%) in HAL-treated skin than MAL-treated skin, although not significant (P = ns). At skin surface, photobleaching was significantly higher (90-98%) after LED illumination (18 and 37 J/cm²) than IPL (29-53%, 36 and 72 J/cm²) and LPDL (43-62%, 7 and 15 J/cm²) (P epidermis to deep dermis by LED illumination (37 J/cm², P = ns), but declined from epidermis to mid and deep dermis for IPL-treated skin and LPDL-treated skin (IPL 72 J/cm²: 26-15%; LPDL 15 J/cm²: 37-23%) (P < 0.04). Clinically, erythema correlated linearly with MAL and HAL-induced photobleaching (r² = 0.175, P < 0.001). In conclusion, selective PpIX accumulation indicates HAL as an alternative to MAL for epidermal-targeted PDT. In clinically relevant doses, PpIX photobleaching throughout the skin was more profound following LED than LPDL and IPL exposure.

  9. Investigating an approach to identifying the biomechanical differences between intercostal cartilage in subjects with pectus excavatum and normals in vivo: preliminary assessment of normal subjects

    Science.gov (United States)

    Rechowicz, Krzysztof; McKenzie, Frederic; Yan, Zhenzhen; Bawab, Sebastian; Ringleb, Stacie

    2009-02-01

    The cause of pectus excavatum (PE) is unknown and little research has been done to assess the material properties of the PE costal cartilage. One source reported, after studying ex vivo various properties of the costal cartilage in cases of PE that the biomechanical stability of PE cartilage is decreased when compared to that of normals. Building on this idea, it would be beneficial to measure the biomechanical properties of the costal cartilages in vivo to further determine the differences between PE subjects and normals. An approach to doing this would be to use a modified FARO arm, which can read applied loads and resulting deflections. These values can be used to establish a finite element model of the chest area of a person with PE. So far, a validated technique for the registration between a CT based 3D model of the ribcage and a skin surface scan in case of PE has been addressed. On the basis of the data gathered from 10 subjects with normal chests using a robot arm, stylus and 3D laser scanner, we tried to evaluate the influence of inter-measurement respiration of a subject on results accuracy and the possibility of using the stylus for deflection measurement. In addition, we established the best strategy for taking measurements.

  10. Reproducibility of VPCT parameters in the normal pancreas: comparison of two different kinetic calculation models.

    Science.gov (United States)

    Kaufmann, Sascha; Schulze, Maximilian; Horger, Thomas; Oelker, Aenne; Nikolaou, Konstantin; Horger, Marius

    2015-09-01

    To assess the reproducibility of volume computed tomographic perfusion (VPCT) measurements in normal pancreatic tissue using two different kinetic perfusion calculation models at three different time points. Institutional ethical board approval was obtained for retrospective analysis of pancreas perfusion data sets generated by our prospective study for liver response monitoring to local therapy in patients experiencing unresectable hepatocellular carcinoma, which was approved by the institutional review board. VPCT of the entire pancreas was performed in 41 patients (mean age, 64.8 years) using 26 consecutive volume measurements and intravenous injection of 50 mL of iodinated contrast at a flow rate of 5 mL/s. Blood volume(BV) and blood flow (BF) were calculated using two mathematical methods: maximum slope + Patlak analysis versus deconvolution method. Pancreas perfusion was calculated using two volume of interests. Median interval between the first and the second VPCT was 2 days and between the second and the third VPCT 82 days. Variability was assessed with within-patient coefficients of variation (CVs) and Bland-Altman analyses. Interobserver agreement for all perfusion parameters was calculated using intraclass correlation coefficients (ICCs). BF and BV values varied widely by method of analysis as did within-patient CVs for BF and BV at the second versus the first VPCT by 22.4%/50.4% (method 1) and 24.6%/24.0% (method 2) measured in the pancreatic head and 18.4%/62.6% (method 1) and 23.8%/28.1% (method 2) measured in the pancreatic corpus and at the third versus the first VPCT by 21.7%/61.8% (method 1) and 25.7%/34.5% (method 2) measured also in the pancreatic head and 19.1%/66.1% (method 1) and 22.0%/31.8% (method 2) measured in the pancreatic corpus, respectively. Interobserver agreement measured with ICC shows fair-to-good reproducibility. VPCT performed with the presented examinational protocol is reproducible and can be used for monitoring

  11. Grouping normal type Ia supernovae by UV to optical color differences

    Energy Technology Data Exchange (ETDEWEB)

    Milne, Peter A. [University of Arizona, Steward Observatory, 933 North Cherry Avenue, Tucson, AZ 85719 (United States); Brown, Peter J. [George P. and Cynthia Woods Mitchell Institute for Fundamental Physics and Astronomy, Texas A. and M. University, Department of Physics and Astronomy, 4242 TAMU, College Station, TX 77843 (United States); Roming, Peter W. A. [Space Science and Engineering Division, Southwest Research Corporation, P.O. Drawer 28510, San Antonio, TX 78228-0510 (United States); Bufano, Filomena [Universidad Andres Bello, Departmento de Cincias Fisicas, Avda. Republica 220, Santiago (Chile); Gehrels, Neil, E-mail: pbrown@physics.tamu.edu [NASA-Goddard Space Flight Center, Astrophysics Science Division, Codes 660.1 and 662, Greenbelt, MD 20771 (United States)

    2013-12-10

    Observations of many Type Ia supernovae (SNe Ia) for multiple epochs per object with the Swift Ultraviolet Optical Telescope instrument have revealed that there exists order to the differences in the UV-optical colors of optically normal supernovae (SNe). We examine UV-optical color curves for 23 SNe Ia, dividing the SNe into four groups, and find that roughly one-third of 'NUV-blue' SNe Ia have bluer UV-optical colors than the larger 'NUV-red' group. Two minor groups are recognized, 'MUV-blue' and 'irregular' SNe Ia. While we conclude that the latter group is a subset of the NUV-red group, containing the SNe with the broadest optical peaks, we conclude that the 'MUV-blue' group is a distinct group. Separating into the groups and accounting for the time evolution of the UV-optical colors lowers the scatter in two NUV-optical colors (e.g., u – v and uvw1 – v) to the level of the scatter in b – v. This finding is promising for extending the cosmological utilization of SNe Ia into the NUV. We generate spectrophotometry of 33 SNe Ia and determine the correct grouping for each. We argue that there is a fundamental spectral difference in the 2900-3500 Å wavelength range, a region suggested to be dominated by absorption from iron-peak elements. The NUV-blue SNe Ia feature less absorption than the NUV-red SNe Ia. We show that all NUV-blue SNe Ia in this sample also show evidence of unburned carbon in optical spectra, whereas only one NUV-red SN Ia features that absorption line. Every NUV-blue event also exhibits a low gradient of the Si II λ6355 absorption feature. Many NUV-red events also exhibit a low gradient, perhaps suggestive that NUV-blue events are a subset of the larger low-velocity gradient group.

  12. Differences in ( sup 14 C)glycerol utilization in normal and familial hypercholesterolemic fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Shireman, R.B.; Durieux, J. (Univ. of Florida Gainesville (USA))

    1991-01-01

    It is known that cultured fibroblasts from familial hypercholesterolemia (FH) patients lack the normal cell receptor for low density lipoprotein (LDL) and that the absence of receptor-mediated transport of LDL cholesterol into these cells results in increased cellular synthesis of cholesterol. After 20 h perincubation in lipid-free medium, cultured FH fibroblasts incorporated significantly greater amounts of ({sup 14}C)glycerol into cellular lipids than did normal fibroblasts. Relative to the control medium which contained only bovine serum albumin (BSA), preincubation with 5% fetal bovine serum or 50 micrograms LDL/ml decreased ({sup 14}C)glycerol incorporation by both cell types. FH cells utilized more ({sup 14}C)glycerol for phospholipid synthesis and less for triglyceride synthesis than normal cells. This study indicates that LDL may be important in the transport of glycerides, as well as cholesterol, to cells.

  13. Protein differences between normal and oligospermic human sperm demonstrated by two-dimensional gel electrophoresis.

    Science.gov (United States)

    Morgentaler, A; Schopperle, W M; Crocker, R H; DeWolf, W C

    1990-11-01

    Protein expression by sperm obtained from men with normal semen analysis and men with oligospermia were evaluated by two-dimensional gel electrophoresis. Proteins were solubilized in a 9.5 M urea/2% Nonidet-P40 (LKB, Bromma, Sweden) lysis buffer and underwent second dimension separation on 10 to 16% polyacrylamide gradient gels. A set of 36 invariant proteins was identified in all normospermic samples, whereas 8 of 10 evaluable oligospermic samples lacked 1 or more of the invariant proteins. Proteins absent in oligospermic samples may be critical to normal sperm function and may serve as markers for infertility.

  14. Comparing the Normalized Difference Infrared Index (NDII) with root zone storage in a lumped conceptual model

    Science.gov (United States)

    Sriwongsitanon, Nutchanart; Gao, Hongkai; Savenije, Hubert H. G.; Maekan, Ekkarin; Saengsawang, Sirikanya; Thianpopirug, Sansarith

    2016-08-01

    With remote sensing we can readily observe the Earth's surface, but direct observation of the sub-surface remains a challenge. In hydrology, but also in related disciplines such as agricultural and atmospheric sciences, knowledge of the dynamics of soil moisture in the root zone of vegetation is essential, as this part of the vadose zone is the core component controlling the partitioning of water into evaporative fluxes, drainage, recharge, and runoff. In this paper, we compared the catchment-scale soil moisture content in the root zone of vegetation, computed by a lumped conceptual model, with the remotely sensed Normalized Difference Infrared Index (NDII) in the Upper Ping River basin (UPRB) in northern Thailand. The NDII is widely used to monitor the equivalent water thickness (EWT) of leaves and canopy. Satellite data from the Moderate Resolution Imaging Spectroradiometer (MODIS) were used to determine the NDII over an 8-day period, covering the study area from 2001 to 2013. The results show that NDII values decrease sharply at the end of the wet season in October and reach lowest values near the end of the dry season in March. The values then increase abruptly after rains have started, but vary in an insignificant manner from the middle to the late rainy season. This paper investigates if the NDII can be used as a proxy for moisture deficit and hence for the amount of moisture stored in the root zone of vegetation, which is a crucial component of hydrological models. During periods of moisture stress, the 8-day average NDII values were found to correlate well with the 8-day average soil moisture content (Su) simulated by the lumped conceptual hydrological rainfall-runoff model FLEX for eight sub-catchments in the Upper Ping basin. Even the deseasonalized Su and NDII (after subtracting the dominant seasonal signal) showed good correlation during periods of moisture stress. The results illustrate the potential of the NDII as a proxy for catchment-scale root zone

  15. Control of interjoint coordination during the swing phase of normal gait at different speeds

    Directory of Open Access Journals (Sweden)

    Gottlieb Gerald L

    2007-04-01

    Full Text Available Abstract Background It has been suggested that the control of unconstrained movements is simplified via the imposition of a kinetic constraint that produces dynamic torques at each moving joint such that they are a linear function of a single motor command. The linear relationship between dynamic torques at each joint has been demonstrated for multijoint upper limb movements. The purpose of the current study was to test the applicability of such a control scheme to the unconstrained portion of the gait cycle – the swing phase. Methods Twenty-eight neurologically normal individuals walked along a track at three different speeds. Angular displacements and dynamic torques produced at each of the three lower limb joints (hip, knee and ankle were calculated from segmental position data recorded during each trial. We employed principal component (PC analysis to determine (1 the similarity of kinematic and kinetic time series at the ankle, knee and hip during the swing phase of gait, and (2 the effect of walking speed on the range of joint displacement and torque. Results The angular displacements of the three joints were accounted for by two PCs during the swing phase (Variance accounted for – PC1: 75.1 ± 1.4%, PC2: 23.2 ± 1.3%, whereas the dynamic joint torques were described by a single PC (Variance accounted for – PC1: 93.8 ± 0.9%. Increases in walking speed were associated with increases in the range of motion and magnitude of torque at each joint although the ratio describing the relative magnitude of torque at each joint remained constant. Conclusion Our results support the idea that the control of leg swing during gait is simplified in two ways: (1 the pattern of dynamic torque at each lower limb joint is produced by appropriately scaling a single motor command and (2 the magnitude of dynamic torque at all three joints can be specified with knowledge of the magnitude of torque at a single joint. Walking speed could therefore be altered

  16. Near Point of Convergence Break for Different Age Groups in Turkish Population with Normal Binocular Vision: Normative Data

    OpenAIRE

    Nihat Sayın; Birsen Gökyiğit; Pelin Kaynak; Duygu Tüzün Sayın; Ahmet Demirok; Serpil Akar

    2013-01-01

    Purpose: The purpose of this study was to evaluate the near point of convergence break in Turkish population with normal binocular vision and to obtain the normative data for the near point of convergence break in different age groups. Such database has not been previously reported. Material and Method: In this prospective study, 329 subjects with normal binocular vision (age range, 3-72 years) were evaluated. The near point of convergence break was measured 4 times repeatedly wit...

  17. Market size structure and small business lending : Are crisis times different from normal times?

    NARCIS (Netherlands)

    Berger, Allen N.; Cerqueiro, G.M.; Penas, Maria

    2015-01-01

    Conventional wisdom holds that small banks have comparative advantages vis-à-vis large banks in serving small firms, while recent literature suggests this may not be the case. Using a panel of recent US start-ups, we investigate how small bank presence affects these firms in normal times (2004–06) a

  18. Brain gray and white matter differences in healthy normal weight and obese children

    Science.gov (United States)

    To compare brain gray and white matter development in healthy normal weight and obese children. Twenty-four healthy 8- to 10-year-old children whose body mass index was either 95th percentile (obese) completed an MRI examination which included T1-weighted three-d...

  19. An experimental randomized study of six different ventilatory modes in a piglet model with normal lungs

    DEFF Research Database (Denmark)

    Nielsen, J B; Sjöstrand, U H; Henneberg, S W

    1991-01-01

    A randomized study of 6 ventilatory modes was made in 7 piglets with normal lungs. Using a Servo HFV 970 (prototype system) and a Servo ventilator 900 C the ventilatory modes examined were as follows: SV-20V, i.e. volume-controlled intermittent positive-pressure ventilation (IPPV); SV-20VIosc, i...

  20. Body composition differences in normal weight, obese-overweight and anorexic adolescents: role of adipocytokines.

    Science.gov (United States)

    Ondrak, Kristin S; Hackney, Anthony C

    2010-01-01

    Adipocytokines are signaling molecules released by adipose tissue with numerous functions, including regulation of metabolism, inflammatory process, and body mass. They are particularly interesting in youth, considering the rising prevalence of overweight/obesity and the linkage of this condition to inflammation. This chapter examines the relationship between body composition and select adipocytokines: leptin, adiponectin, IL-6, TNF-α, and resistin, in overweight, normal weight and anorexic youth. Leptin, which stimulates energy expenditure and promotes satiety, is highest in overweight youth, followed by normal weight and lastly anorexic youth. Adiponectin has similar functions to leptin but is negatively correlated with measures of body composition. Anorexic youth have the highest adiponectin per kg fat mass, followed by normal weight and overweight. Conversely, IL-6 is positively associated with body composition; however, research in anorexic youth is inconclusive. It has some pro-inflammatory effects and promotes glucose and fat use, therefore beneficial for maintenance of normal weight status. TNF-α is also a pro-inflammatory adipocytokine thought to be somewhat protective against cancer. TNF-α is highest in overweight, followed by normal weight and anorexic youth, similar to leptin. Finally, resistin is also involved in the pro-inflammatory response and the development of insulin resistance. However, far less research exists on this adipocytokine and its relation to body composition in overweight or anorexic youth is equivocal. In conclusion, several consistent relationships exist regarding adipocytokines and body composition; however, there is a need for additional research on these relationships in youth especially at extremes of adiposity such as overweight and anorexics. Copyright © 2010 S. Karger AG, Basel.

  1. Differences in MITF gene expression and histology between albino and normal sea cucumbers ( Apostichopus japonicus Selenka)

    Science.gov (United States)

    Zhao, Heling; Yang, Hongsheng; Zhao, Huan; Liu, Shilin; Wang, Tianming

    2012-01-01

    Albino Apostichopus japonicus occur both in the wild and in captivity. The offspring of albino A. japonicus also suffer from albinism. The formation of melanin in the melanocytes is dependant on microphthalmia-associated transcription factor (MITF). To investigate the role of MITF in controlling albinism, we cloned the full-length MITF cDNA from A. japonicus and compared MITF mRNA expression in albino and normal A. japonicus. In addition, we used light and electron microscopy to compare histological samples of normal and albino A. japonicus. The body wall of albino adults was characterized by significantly lower levels of MITF expression and lower numbers of epidermal melanocytes, which also contained less melanin. In albino juvenile offspring, MITF expression levels were significantly lower 32 d after fertilization and there were fewer, and less developed, epidermal melanocytes. Thus, we conclude that albino A. japonicus have fewer melanocytes and a reduced ability to synthesize melanin, likely because of lower expression of MITF.

  2. Proteome Differences in Placenta and Endometrium between Normal and Intrauterine Growth Restricted Pig Fetuses.

    Directory of Open Access Journals (Sweden)

    Fang Chen

    Full Text Available Uteroplacental tissue plays a key role in substance exchanges between maternal and fetal circulation, and, therefore, in the growth and development of fetuses. In this study, proteomics and western blotting were applied to investigate the changes of proteome in the placenta and endometrium of normal and intrauterine growth restriction (IUGR porcine fetuses during mid to late pregnancy (D60, 90, and 110 of gestation. Our results showed that proteins participating in cell structure, energy metabolism, stress response, cell turnover, as well as transport and metabolism of nutrients were differentially expressed in placenta and endometrium between normal and IUGR fetuses. Analysis of functions of these proteins suggests reductions in ATP production and nutrients transport, increases in oxidative stress and apoptosis, and impairment of cell metabolism in IUGR fetuses. Collectively, our findings aid in understanding of the mechanisms responsible for uteroplacental dysfunction in IUGR fetus, and are expected to provide new strategies to reduce fetal growth restriction in pigs and other mammals.

  3. How robust are in situ observations for validating satellite-derived albedo over the dark zone of the Greenland Ice Sheet?

    Science.gov (United States)

    Ryan, J. C.; Hubbard, A.; Irvine-Fynn, T. D.; Doyle, S. H.; Cook, J. M.; Stibal, M.; Box, J. E.

    2017-06-01

    Calibration and validation of satellite-derived ice sheet albedo data require high-quality, in situ measurements commonly acquired by up and down facing pyranometers mounted on automated weather stations (AWS). However, direct comparison between ground and satellite-derived albedo can only be justified when the measured surface is homogeneous at the length-scale of both satellite pixel and in situ footprint. Here we use digital imagery acquired by an unmanned aerial vehicle to evaluate point-to-pixel albedo comparisons across the western, ablating margin of the Greenland Ice Sheet. Our results reveal that in situ measurements overestimate albedo by up to 0.10 at the end of the melt season because the ground footprints of AWS-mounted pyranometers are insufficient to capture the spatial heterogeneity of the ice surface as it progressively ablates and darkens. Statistical analysis of 21 AWS across the entire Greenland Ice Sheet reveals that almost half suffer from this bias, including some AWS located within the wet snow zone.

  4. Pore size determination using normalized J-function for different hydraulic flow units

    Directory of Open Access Journals (Sweden)

    Ali Abedini

    2015-06-01

    Full Text Available Pore size determination of hydrocarbon reservoirs is one of the main challenging areas in reservoir studies. Precise estimation of this parameter leads to enhance the reservoir simulation, process evaluation, and further forecasting of reservoir behavior. Hence, it is of great importance to estimate the pore size of reservoir rocks with an appropriate accuracy. In the present study, a modified J-function was developed and applied to determine the pore radius in one of the hydrocarbon reservoir rocks located in the Middle East. The capillary pressure data vs. water saturation (Pc–Sw as well as routine reservoir core analysis include porosity (φ and permeability (k were used to develop the J-function. First, the normalized porosity (φz, the rock quality index (RQI, and the flow zone indicator (FZI concepts were used to categorize all data into discrete hydraulic flow units (HFU containing unique pore geometry and bedding characteristics. Thereafter, the modified J-function was used to normalize all capillary pressure curves corresponding to each of predetermined HFU. The results showed that the reservoir rock was classified into five separate rock types with the definite HFU and reservoir pore geometry. Eventually, the pore radius for each of these HFUs was determined using a developed equation obtained by normalized J-function corresponding to each HFU. The proposed equation is a function of reservoir rock characteristics including φz, FZI, lithology index (J*, and pore size distribution index (ɛ. This methodology used, the reservoir under study was classified into five discrete HFU with unique equations for permeability, normalized J-function and pore size. The proposed technique is able to apply on any reservoir to determine the pore size of the reservoir rock, specially the one with high range of heterogeneity in the reservoir rock properties.

  5. [Sensitivity of different morphological variants of Leptospira to the leptospirocidal activity of normal animal sera].

    Science.gov (United States)

    Anan'ina, Iu V; Chernukha, Iu G

    1984-10-01

    The leptospirocidal activity of normal animal sera with respect to 23 Leptospira strains was experimentally studied in vitro. 91.3% of the strains under study proved to be sensitive to the lytic action of cattle serum and 86.9%, to sheep serum. The uncinate variants of the pathogenic strains showed resistance to the action of the above sera, and their nonuncinate analogs were subject to agglutination with subsequent lysis, similarly to saprophytes.

  6. Two tails of the normal curve. Similarities and differences in the study of mental retardation and giftedness.

    Science.gov (United States)

    Robinson, N M; Zigler, E; Gallagher, J J

    2000-12-01

    Professionals in the fields of mental retardation and giftedness have much to teach each other as well as the field of human development in general. Examining the commonalities and differences between the fields in social issues, definitions, developmental differences from the norm, values and policy issues, and educational and long-term implications deepens insights about both normal and deviant development. The authors stress the importance of individual differences in the differential design of educational strategies and the application of approaches developed with specialized populations to normally developing children. Current social inequalities affect both of these fields in particular ways. Finally, numerous research agendas can be enhanced by including representatives of both ends of the normal curve.

  7. Comparison of surface energy fluxes with satellite-derived surface energy flux estimates from a shrub-steppe

    Energy Technology Data Exchange (ETDEWEB)

    Kirkham, Randy R. [Univ. of Washington, Seattle, WA (United States)

    1993-12-01

    This thesis relates the components of the surface energy balance (i.e., net radiation, sensible and latent heat flux densities, soil heat flow) to remotely sensed data for native vegetation in a semi-arid environment. Thematic mapper data from Landsat 4 and 5 were used to estimate net radiation, sensible heat flux (H), and vegetation amount. Several sources of ground truth were employed. They included soil water balance using the neutron thermalization method and weighing lysimeters, and the measurement of energy fluxes with the Bowen ratio energy balance (BREB) technique. Sensible and latent heat flux were measured at four sites on the U.S. Department of Energy`s Hanford Site using a weighing lysimeter and/or BREB stations. The objective was to calibrate an aerodynamic transport equation that related H to radiant surface temperature. The transport equation was then used with Landsat thermal data to generate estimates of H and compare these estimates against H values obtained with BREB/lysimeters at the time of overflight. Landsat and surface meteorologic data were used to estimate the radiation budget terms at the surface. Landsat estimates of short-wave radiation reflected from the surface correlate well with reflected radiation measured using inverted Eppley pyranometers. Correlation of net radiation estimates determined from satellite data, pyranometer, air temperature, and vapor pressure compared to net radiometer values obtained at time of overflight were excellent for a single image, but decrease for multiple images. Soil heat flux, GT, is a major component of the energy balance in arid systems and G{sub T} generally decreases as vegetation cover increases. Normalized difference vegetation index (NDVI) values generated from Landsat thermatic mapper data were representative of field observations of the presence of green vegetation, but it was not possible to determine a single relationship between NDVI and GT for all sites.

  8. Strong contribution of autumn phenology to changes in satellite-derived growing season length estimates across Europe (1982-2011).

    Science.gov (United States)

    Garonna, Irene; de Jong, Rogier; de Wit, Allard J W; Mücher, Caspar A; Schmid, Bernhard; Schaepman, Michael E

    2014-11-01

    Land Surface Phenology (LSP) is the most direct representation of intra-annual dynamics of vegetated land surfaces as observed from satellite imagery. LSP plays a key role in characterizing land-surface fluxes, and is central to accurately parameterizing terrestrial biosphere-atmosphere interactions, as well as climate models. In this article, we present an evaluation of Pan-European LSP and its changes over the past 30 years, using the longest continuous record of Normalized Difference Vegetation Index (NDVI) available to date in combination with a landscape-based aggregation scheme. We used indicators of Start-Of-Season, End-Of-Season and Growing Season Length (SOS, EOS and GSL, respectively) for the period 1982-2011 to test for temporal trends in activity of terrestrial vegetation and their spatial distribution. We aggregated pixels into ecologically representative spatial units using the European Landscape Classification (LANMAP) and assessed the relative contribution of spring and autumn phenology. GSL increased significantly by 18-24 days decade(-1) over 18-30% of the land area of Europe, depending on methodology. This trend varied extensively within and between climatic zones and landscape classes. The areas of greatest growing-season lengthening were the Continental and Boreal zones, with hotspots concentrated in southern Fennoscandia, Western Russia and pockets of continental Europe. For the Atlantic and Steppic zones, we found an average shortening of the growing season with hotspots in Western France, the Po valley, and around the Caspian Sea. In many zones, changes in the NDVI-derived end-of-season contributed more to the GSL trend than changes in spring green-up, resulting in asymmetric trends. This underlines the importance of investigating senescence and its underlying processes more closely as a driver of LSP and global change.

  9. Dynamic Response of Satellite-Derived Vegetation Growth to Climate Change in the Three North Shelter Forest Region in China

    Directory of Open Access Journals (Sweden)

    Bin He

    2015-08-01

    Full Text Available Since the late 1970s, the Chinese government has initiated ecological restoration programs in the Three North Shelter Forest System Project (TNSFSP area. Whether accelerated climate change will help or hinder these efforts is still poorly understood. Using the updated and extended AVHRR NDVI3g dataset from 1982 to 2011 and corresponding climatic data, we investigated vegetation variations in response to climate change. The results showed that the overall state of vegetation in the study region has improved over the past three decades. Vegetation cover significantly decreased in 23.1% and significantly increased in 21.8% of the study area. An increase in all three main vegetation types (forest, grassland, and cropland was observed, but the trend was only statistically significant in cropland. In addition, bare and sparsely vegetated areas, mainly located in the western part of the study area, have significantly expanded since the early 2000s. A moisture condition analysis indicated that the study area experienced significant climate variations, with warm-wet conditions in the western region and warm-dry conditions in the eastern region. Correlation analysis showed that variations in the Normalized Difference Vegetation Index (NDVI were positively correlated with precipitation and negatively correlated with temperature. Ultimately, climate change influenced vegetation growth by controlling the availability of soil moisture. Further investigation suggested that the positive impacts of precipitation on NDVI have weakened in the study region, whereas the negative impacts from temperature have been enhanced in the eastern study area. However, over recent years, the negative temperature impacts have been converted to positive impacts in the western region. Considering the variations in the relationship between NDVI and climatic variables, the warm–dry climate in the eastern region is likely harmful to vegetation growth, whereas the warm

  10. Differences in collagen architecture between keloid, hypertrophic scar, normotrophic scar, and normal skin: An objective histopathological analysis.

    Science.gov (United States)

    Verhaegen, Pauline D H M; van Zuijlen, Paul P M; Pennings, Noor M; van Marle, Jan; Niessen, Frank B; van der Horst, Chantal M A M; Middelkoop, Esther

    2009-01-01

    Normotrophic, hypertrophic, and keloidal scars are different types of scar formation, which all need a different approach in treatment. Therefore, it is important to differentiate between these types of scar, not only clinically but also histopathologically. Differences were explored for collagen orientation and bundle thickness in 25 normal skin, 57 normotrophic scar, 56 hypertrophic scar, and 56 keloid biopsies, which were selected on clinical diagnosis. Image analysis was performed by fast fourier transformation. The calculated collagen orientation index ranged from 0 (random orientation) to 1 (parallel orientation). The bundle distance was calculated by the average distance between the centers of the collagen bundles. The results showed that compared with all three types of scars, the collagen orientation index was significantly lower in normal skin, which indicates that scars are organized in a more parallel manner. No differences were found between the different scars. Secondly, compared with normal skin, normotrophic scar, and hypertrophic scar, the bundle distance was significantly larger in keloidal scar, which suggests that thicker collagen bundles are present in keloidal scar. This first extensive histological study showed objective differences between normal skin, normotrophic, hypertrophic, and keloidal scar.

  11. Apparent diffusion coefficient normalization of normal liver: Will it improve the reproducibility of diffusion-weighted imaging at different MR scanners as a new biomarker?

    Science.gov (United States)

    Zhu, Jie; Zhang, Jie; Gao, Jia-Yin; Li, Jin-Ning; Yang, Da-Wei; Chen, Min; Zhou, Cheng; Yang, Zheng-Han

    2017-01-01

    Apparent diffusion coefficient (ADC) measurement in diffusion-weighted imaging (DWI) has been reported to be a helpful biomarker for detection and characterization of lesion. In view of the importance of ADC measurement reproducibility, the aim of this study was to probe the variability of the healthy hepatic ADC values measured at 3 MR scanners from different vendors and with different field strengths, and to investigate the reproducibility of normalized ADC (nADC) value with the spleen as the reference organ. Thirty enrolled healthy volunteers received DWI with GE 1.5T, Siemens 1.5T, and Philips 3.0T magnetic resonance (MR) systems on liver and spleen (session 1) and were imaged again after 10 to 14 days using only GE 1.5T MR and Philips 3.0T MR systems (session 2). Interscan agreement and reproducibility of ADC measurements of liver and the calculated nADC values (ADCliver/ADCspleen) were statistically evaluated between 2 sessions. In session 1, ADC and nADC values of liver were evaluated for the scanner-related variability by 2-way analysis of variance and intraclass correlation coefficients (ICCs). Coefficients of variation (CVs) of ADCs and nADCs of liver were calculated for both 1.5 and 3.0-T MR system. Interscan agreement and reproducibility of ADC measurements of liver and related nADCs between 2 sessions were found to be satisfactory with ICC values of 0.773 to 0.905. In session 1, the liver nADCs obtained from different scanners were consistent (P = 0.112) without any significant difference in multiple comparison (P = 0.117 to >0.99) by using 2-way analysis of variance with post-hoc analysis of Bonferroni method, although the liver ADCs varied significantly (P < 0.001). nADCs measured by 3 scanners were in good interscanner agreements with ICCs of 0.685 to 0.776. The mean CV of nADCs of both 1.5T MR scanners (9.6%) was similar to that of 3.0T MR scanner (8.9%). ADCs measured at 3 MR scanners with different field strengths and vendors could

  12. Differences in regional air trapping in current smokers with normal spirometry.

    Science.gov (United States)

    Karimi, Reza; Tornling, Göran; Forsslund, Helena; Mikko, Mikael; Wheelock, Åsa M; Nyrén, Sven; Sköld, C Magnus

    2017-01-01

    We investigated regional air trapping on computed tomography in current smokers with normal spirometry. It was hypothesised that presence of regional air trapping may indicate a specific manifestation of smoking-related changes.40 current smokers, 40 patients with chronic obstructive pulmonary disease (COPD), and 40 healthy never- smokers underwent computed tomography scans. Regional air trapping was assessed on end-expiratory scans and emphysema, micronodules and bronchial wall thickening on inspiratory scans. The ratio of expiratory and inspiratory mean lung attenuation (E/I) was calculated as a measure of static (fixed) air trapping.Regional air trapping was present in 63% of current smokers, in 45% of never smokers and in 8% of COPD patients (p<0.001). Current smokers with and without regional air trapping had E/I ratio of 0.81 and 0.91, respectively (p<0.001). Forced expiratory volume in 1 s (FEV1) was significantly higher and emphysema less frequent in current smokers with regional air trapping.Current smokers with regional air trapping had higher FEV1 and less emphysema on computed tomography. In contrast, current smokers without regional air trapping resembled COPD. Our results highlight heterogeneity among smokers with normal spirometry and may contribute to early detection of smoking related structural changes in the lungs.

  13. Differences in MITF gene expression and histology between albino and normal sea cucumbers (Apostichopus japonicus Selenka)

    Institute of Scientific and Technical Information of China (English)

    ZHAO Heling; YANG Hongsheng; ZHAO Huan; LIU Shilin; WANG Tianming

    2012-01-01

    Albino Apostichopus japonicus occur both in the wild and in captivity.The offspring of albino A.japonicus also suffer from albinism.The formation of melanin in the melanocytes is dependant on microphthalmia-associated transcription factor (MITF).To investigate the role of MITF in controlling albinism,we cloned the full-length MITF cDNA from A.japonicus and compared MITF mRNA expression in albino and normal A.japonicus.In addition,we used light and electron microscopy to compare histological samples of normal and albino A.japonicus.The body wall of albino adults was characterized by significantly lower levels of MITF expression and lower numbers of epidermal melanocytes,which also contained less melanin.In albino juvenile offspring,MITF expression levels were significantly lower 32 d after fertilization and there were fewer,and less developed,epidermal melanocytes.Thus,we conclude that albino A.japonicus have fewer melanocytes and a reduced ability to synthesize melanin,likely because of lower expression of MITF.

  14. Comparing journals from different fields of Science and Social Science through a JCR Subject Categories Normalized Impact Factor

    CERN Document Server

    Dorta-Gonzalez, Pablo; 10.1007/s11192-012-0929-9

    2013-01-01

    The journal Impact Factor (IF) is not comparable among fields of Science and Social Science because of systematic differences in publication and citation behaviour across disciplines. In this work, a decomposing of the field aggregate impact factor into five normally distributed variables is presented. Considering these factors, a Principal Component Analysis is employed to find the sources of the variance in the JCR subject categories of Science and Social Science. Although publication and citation behaviour differs largely across disciplines, principal components explain more than 78% of the total variance and the average number of references per paper is not the primary factor explaining the variance in impact factors across categories. The Categories Normalized Impact Factor (CNIF) based on the JCR subject category list is proposed and compared with the IF. This normalization is achieved by considering all the indexing categories of each journal. An empirical application, with one hundred journals in two ...

  15. Effects of Normal and Perturbed Social Play on the Duration and Amplitude of Different Types of Infant Smiles

    Science.gov (United States)

    Fogel, Alan; Hsu, Hui-Chin; Shapiro, Alyson F.; Nelson-Goens, G. Christina; Secrist, Cory

    2006-01-01

    Different types of smiling varying in amplitude of lip corner retraction were investigated during 2 mother-infant games--peekaboo and tickle--at 6 and 12 months and during normally occurring and perturbed games. Using Facial Action Coding System (FACS), infant smiles were coded as simple (lip corner retraction only), Duchenne (simple plus cheek…

  16. Evaluation of different normalization procedures for the calculation of the standardized uptake value in therapy response monitoring studies

    NARCIS (Netherlands)

    Vriens, Dennis; de Geus-Oei, Lioe-Fee; van Laarhoven, Hanneke W; Timmer-Bonte, Johanna N H; Krabbe, Paul F M; Visser, Eric P; Oyen, Wim J G

    2009-01-01

    OBJECTIVES: The aim of this prospective study was to assess the influence of different normalization procedures on relative changes in standardized uptake values (SUV) of F-2-fluoro-2-deoxy-D-glucose positron emission tomography (FDG-PET) for the assessment of chemotherapy response in patients with

  17. The composition of trigeminal nerve branches in normal adult chickens and after debeaking at different ages.

    Science.gov (United States)

    Dubbeldam, J L; De Bakker, M A; Bout, R G

    1995-06-01

    The long term effects of amputation of the tip of the beak were studied in adult hens that were debeaked on the day of hatching, at the age of 8 d and at 6 wk, by EM analysis of fibre spectra of the medial branch of the ophthalmic nerve and of the intramandibular nerve. Three categories of fibre were distinguished for further analysis, i.e. unmyelinated axons, small myelinated fibres and large myelinated fibres. In normal birds the ophthalmic nerve contains relatively more large fibres than the intramandibular nerve. Amputation consistently results in a reduction of the number of large fibres and a substantial increase in the number of small myelinated fibres. The proportion of unmyelinated axons is rather variable, but is not affected by beak trimming. Age at debeaking has no effect. The observations are inconclusive concerning the possibility of heightened nociception.

  18. Differences in the interaction of acetylcholine receptor antibodies with receptor from normal, denervated and myasthenic human muscle.

    OpenAIRE

    Lefvert, A. K.

    1982-01-01

    The interaction of acetylcholine receptor antibodies with different kinds of human skeletal muscle receptor was investigated. The reaction of most receptor antibodies was strongest with receptor from a patient with myasthenia gravis and with receptor from denervated muscle. Results obtained with these receptors were well correlated. The binding of most receptor antibodies to receptor from functionally normal muscle was much weaker and also qualitatively different. In a few patients with moder...

  19. Quantitative and qualitative differences in DNA complementary to avian myeloblastosis virus between normal and leukemic chicken cells.

    Science.gov (United States)

    Baluda, M A; Shoyab, M; Evans, R; Markham, P D; Ali, M

    1975-01-01

    Hybridization of avian myeloblastosis virus (AMV) RNA with DNA immobilized on filters or in liquid with a vast DNA excess was used to measure the viral specific DNA sequences in chicken cells. Newly synthesized viral DNA (v-DNA) appears within an hour after infection of chicken embryo fibroblasts (CEF) with avian oncornaviruses. A fraction of newly synthesized v-DNA becomes integrated into the cellular genome and the remainder gradually disappears. A covalent linkage between v-DNA and cellular DNA was demonstrated to exist in CEF and in leukemic myeloblasts by alkaline sucrose velocity sedimentation. Hybridization of AMV RNA in DNA excess has revealed that there are 2 clases of viral specific sequences within normal as well as in leukemic cells. The 2 types of sequences differ in their rate of hybridization. The amount of both types of DNA sequences is about 2 times higher in leukemic cells than in normal cells. Both the fast- and slowly reacting sequences in leukemic cells exhibit a higher Tm (2 degrees C) than the respective DNA sequences in normal cells. Furthermore, when nucleotide sequences in AMV RNA complementary to normal DNA are removed first by exhaustive hybridization with normal DNA, the residual RNA only hybridizes with leukemic DNA but not with normal DNA. These results suggest that leukemic cells contain viral specific DNA sequences which are absent in normal cells. Endogenous v-DNA has been shown to be integrated in cellular DNA region(s) with a reiteration frequency of approximately 1,200 copies per cell and each integration unit appears to have a size approximately equivalent to the 35S RNA subunit of the viral genome. Viral sequences acquired after infection appear to be integrated in the unique region of cell DNA, or in tandem with the endogenous viral sequences.

  20. Increased prospective memory interference in normal and pathological aging: different roles of motor and verbal processing speed.

    Science.gov (United States)

    Gao, J L; Cheung, R T F; Chan, Y S; Chu, L W; Lee, T M C

    2013-01-01

    This is a study on prospective memory (PM) and the PM interference effect in normal and pathological aging. One hundred and seven subjects, including 41 healthy young adults, 40 non-demented older adults and 26 patients with mild Alzheimer's disease (AD) participated in this study using a laboratory event-based PM task. PM task performance was comparable between the non-demented older and young adults, but impaired in the AD patients. The PM interference effect increased progressively from the healthy young adults, the non-demented older adults, to the AD patients. Path analysis revealed that the possible mechanism mediating the increased PM interference was the slow motor processing speed in normal aging, while it was the slow verbal speed in pathological aging. It is suggested that different neuropsychological mechanisms may underpin the affected performance of PM task in normal and pathological aging.

  1. Comparative study on different models for estimation of direct normal irradiance (DNI) over Egypt atmosphere

    Energy Technology Data Exchange (ETDEWEB)

    Madkour, M.A.; Hamed, A.B. [Physics Department, Faculty of Science, Mansoura University (Egypt); El-Metwally, M. [Physics Department, Faculty of Education at Suez, Suez Canal University, Suez (Egypt)

    2006-03-01

    The results obtained by using seven-parameterization broadband models to estimate Direct Normal Irradiance (DNI) along with two spectral models for four sites in Egypt atmosphere were compared with ground DNI measurements. Some statistical indicators (MBE, RMSE and R{sup 2}) have been used to measure the performance of the used models. MBE for all dataset is <1% to both spectral models (SPCTRAL2, SMARTS2) and broadband models (MLWT1, MLWT2 and REST) while is equal to 1.2% to YANG model. However, RMSE are around 2% for spectral models and 3% to the broadband models. The error in prediction of DNI to such models is below experimental errors a part from the big number of observations. On the other hand, Louche, Dogniaux and Rodgers models provide relatively bad performance, RMSE are at most cases >4%. Determination coefficient (R{sup 2}) results to all models are near 1.0. If we excluded spectral models, the broadband models MLWT1, MLWT2 and REST along with YANG models provide the best performance in all tests, therefore, those models can be used in Egypt atmosphere. (author)

  2. Sex differences in the effect of MK-801 on normal and spinal cord injured rats.

    Science.gov (United States)

    Nieto-Sampedro, M; Bailón, C; Rivas, F; Moreno, M T

    1991-01-01

    The induction of functional paraplegia in female rats by contusive spinal cord injury was not prevented by compound MK-801. However, the treatment reduced cavitation around the lesion epicenter to 14 mm3 compared to 17 mm3 in untreated controls t-test, P vaginal frotis. Also, beginning 24 h after MK-801 injection, the proportion of PMN increased 400% in female blood, whereas males maintained control values. Arthritis-like joint inflammation was prominent in the toes of female rats, but males were unaffected. After continued treatment with the drug for 15 days, PMN count in female rats decreased and the animals resumed cycling. However, during this period female rats lost 20% of their weight, whereas males gained 26%. One hour after MK-801 injection large increases in blood pressure occurred in both sexes, returning to normal values 2 h later. Hypothermia does not appear to be a factor in the neuroprotective effect of MK-801, but the drug has a number of potentially dangerous side effects, particularly in female rats. Because polymorphonuclear cells are known sources of oxygen free radicals, neuroprotection by MK-801 treatment ought to be much more efficient in males than in females and the drug should be used in combination with a free-radical scavenger.

  3. Analysis of differences of gene expressions in keloid and normal skin with the aid of cDNA microarray

    Institute of Scientific and Technical Information of China (English)

    Chen Wei; Fu Xiaobing; Sun Xiaoqing; Sun Tongzhu; Zhao Zhili; Yang Yinhui; Sheng Zhiyong

    2003-01-01

    Background: Microarray analysis is a popular tool to investigate the function of genes that are responsible for the phenotype of the disease. Keloid is a intricate lesion which is probably modulated by interplay of many genes. We ventured to study the differences of gene expressions between keloids and normal skins with the aid of cDNA microarray in order to explore the molecular mechanism underlying keloid formation. Methods: The PCR products of 8400 human genes were spotted on a chip in array. The DNAs were then fixed on the glass plate by a series of treatments. Total RNAs was isolated from freshly excised human keloids and normal skin, and then was purified to mRNA by Oligotex. Both the mRNA from keloids and normal skin was reversely transcribed to cDNAs with the incorporations of fluorescent dUTP, for preparing the hybridization probes. The mixed probes were then hybridized to the cDNA microarray. After highly stringent washing, the cDNA microarray was scanned for the fluorescent signals to display the differences between two kinds of tissues. Results: Among 8400 human genes, there were 402 genes (4.79%) with different expression levels between the keloids and normal skins in all cases, 250were up-regulated (2.98%) and 152 down-regulated (1.81%). Analyses of collagen, fibronectin, proteoglycan,growth factors and apoptosis related molecule gene expression confirmed that our molecular data obtained by cDNA microarray were consistent with published biochemical and clinical observations of keloids. Conclusions: DNA microarray technology is an effective technique in screening for differences in gene expression between keloid and normal skin. Many genes are involved in the formation of keloids. Further analysis of the obtained genes will help understand the molecular mechanism of keloid formation.

  4. Decreases in urine specific gravity and urinary creatinine in dogs with different diseases but with normal renal function.

    Science.gov (United States)

    Fojut-Pałka, B; Winnicka, A

    2008-01-01

    The subject of this study was to determine urine specific gravity (USg) and urinary creatinine (UCrn) in dogs with different diseases but with normal renal function. Sick dogs with different diseases were divided into nine groups. Dogs suffering from polyuria/polydipsia, vomits, diarrhoea and females in oestrus or pregnant were excluded from the studies. The healthy dogs served as a control group. Over a three-year period, a total of 267 dogs were examined clinically as well as using imaging and laboratory diagnostics methods. In sick dogs, USg and UCrn were found to be essentially decreased (except animals with neurological and uterine diseases, and neurological diseases, respectively), as compared with normal dogs. In clinically healthy animals of the control group, UCrn and USg did not significantly differ between the females and males. As for the control group, no correlation between UCrn/USg and the body weight/age was found, either.

  5. Fabrication of 14 different RNA nanoparticles for specific tumor targeting without accumulation in normal organs.

    Science.gov (United States)

    Shu, Yi; Haque, Farzin; Shu, Dan; Li, Wei; Zhu, Zhenqi; Kotb, Malak; Lyubchenko, Yuri; Guo, Peixuan

    2013-06-01

    Due to structural flexibility, RNase sensitivity, and serum instability, RNA nanoparticles with concrete shapes for in vivo application remain challenging to construct. Here we report the construction of 14 RNA nanoparticles with solid shapes for targeting cancers specifically. These RNA nanoparticles were resistant to RNase degradation, stable in serum for >36 h, and stable in vivo after systemic injection. By applying RNA nanotechnology and exemplifying with these 14 RNA nanoparticles, we have established the technology and developed "toolkits" utilizing a variety of principles to construct RNA architectures with diverse shapes and angles. The structure elements of phi29 motor pRNA were utilized for fabrication of dimers, twins, trimers, triplets, tetramers, quadruplets, pentamers, hexamers, heptamers, and other higher-order oligomers, as well as branched diverse architectures via hand-in-hand, foot-to-foot, and arm-on-arm interactions. These novel RNA nanostructures harbor resourceful functionalities for numerous applications in nanotechnology and medicine. It was found that all incorporated functional modules, such as siRNA, ribozymes, aptamers, and other functionalities, folded correctly and functioned independently within the nanoparticles. The incorporation of all functionalities was achieved prior, but not subsequent, to the assembly of the RNA nanoparticles, thus ensuring the production of homogeneous therapeutic nanoparticles. More importantly, upon systemic injection, these RNA nanoparticles targeted cancer exclusively in vivo without accumulation in normal organs and tissues. These findings open a new territory for cancer targeting and treatment. The versatility and diversity in structure and function derived from one biological RNA molecule implies immense potential concealed within the RNA nanotechnology field.

  6. Difference in leptin hormone response to nutritional status in normal adult male albino rats.

    Science.gov (United States)

    Al-Sowyan, Noorah S

    2009-01-15

    The present study investigated the effect of 14 days diet, enriched in butter, vitamin E (vit. E) and green tea, on the major regulators of energy expenditure. Leptin is the product OB gene. This 16 KDa protein is produced by mature adipocytes and is secreted in plasma. Its plasma levels are strongly correlated with adipose mass in rodents as well as in humans. Leptin inhibit food intake, reduces body weight and stimulates energy expenditure. In order to evaluate the effect of diet enriched in butter, vit. E and green tea on body weight, adipose tissue weight and organs weight, serum lipids, lipoproteins content and serum leptin levels in male albino rats supplemented for 14 days on the previous diet. This study showed that high fat diet significantly increased body weight and adipose tissue weight, while vit. E and green tea enriched diet significantly lowered body weight and adipose tissue weight, kidney and spleen weights didn't show significant changes in all the experimental groups. While liver weight decreased in diet supplemented with high fat diet. Also, the results showed that high fat diet and vit. E supplemented diet induced significant increase in total cholesterol, LDLc., triglyceride level with significant decrease in HDLc. level as compared to normal control rats. Finally green tea supplemented diet induced significant decrease in total cholesterol, LDLc., triglyceride level with insignificant increase in HDLc. level in control rats. On the other hand, high fat supplemented diet significantly increased serum leptin levels in rats compared to control group, while vit. E and green tea enriched diet significantly lowered serum leptin levels at the end of experimental period. In conclusion, improving the biological activity of leptin by diet modification may exist as a practical strategy for the treatment of obesity and related disorders and a diet rich in green tea to reduce the risk of cardiovascular disease (CVD) obesity and also protect the liver

  7. Threshold Voltage of MOSFET Devices Extracted by Normalized Mutual Integral Difference Operator

    Institute of Scientific and Technical Information of China (English)

    HEJin; ZHENGTaolei; ZHANGXing; WANGYangyuan

    2003-01-01

    The threshold voltage of MOSFETs to be extracted by a novel experimental method named the nor-realized mutual integral difference operator has been inves-tigated in this paper. The basic principle of this method is to use the extreme spectral characteristics of the nor-realized mutual integral difference result of the MOSFET transfer characteristics to find the threshold voltage. Ap-plication of this method has also been demonstrated nu-merically in extracting the threshold voltage of MOS de-vices with the different effective channel length and par-asitic series resistance. The results show this method is sensitive to the channel length variation while insensitive to the parasitic resistance component. The extracted re-sults on the threshold voltage of MOSFET devices have been compared with those obtained by the second deriva-tive method and the agreements have been found, showing the advantage of the method presented here.

  8. Estimation of glacier mass balance: An approach based on satellite-derived transient snowlines and a temperature index driven by meteorological observations

    Science.gov (United States)

    Tawde, S. A.; Kulkarni, A. V.; Bala, G.

    2015-12-01

    In the Himalaya, large area is comprised of glaciers and seasonal snow, mainly due to its high elevated mountain ranges. Long term and continuous assessment of glaciers in this region is important for climatological and hydrological applications. However, rugged terrains and severe weather conditions in the Himalaya lead to paucity in field observations. Therefore, in recent decades, glacier dynamics are extensively monitored using remote sensing in inaccessible terrain like Himalaya. Estimation of glacier mass balance using empirical relationship between mass balance and area accumulation ratio (AAR) requires an accurate estimate of equilibrium-line altitude (ELA). ELA is defined as the snowline at the end of the hydrological year. However, identification of ELA, using remote sensing is difficult because of temporal gaps, cloud cover and intermediate snowfall on glaciers. This leads to large uncertainty in glacier mass-balance estimates by the conventional AAR method that uses satellite-derived highest snowline in ablation season as an ELA. The present study suggests a new approach to improve estimates of ELA location. First, positions of modelled snowlines are optimized using satellite-derived snowlines in the early melt season. Secondly, ELA at the end of the glaciological year is estimated by the melt and accumulation models driven using in situ temperature and precipitation records. From the modelled ELA, mass balance is estimated using the empirical relationship between AAR and mass balance. The modelled mass balance is validated using field measurements on Chhota Shigri and Hamtah glaciers, Himachal Pradesh, India. The new approach shows a substantial improvement in glacier mass-balance estimation, reducing bias by 46% and 108% for Chhota Shigiri and Hamtah glaciers respectively. The cumulative mass loss reconstructed from our approach is 0.85 Gt for nine glaciers in the Chandra basin from 2001 to 2009. The result of the present study is in agreement with

  9. Foot pressure analysis of adults with flat and normal feet at different gait speeds on an ascending slope

    Science.gov (United States)

    Kim, Myoung-Kwon

    2015-01-01

    [Purpose] This study was conducted to determine the difference in foot pressures between flat and normal feet at different gait speeds on an ascending slope. [Subjects] This study enrolled 30 adults with normal (n=15) and flat feet (n=15), with ages from 21 to 30 years old, who had no history of neurological disorders or gait problems. A treadmill was used for the analysis of kinematic features during gait, using a slope of 10%, and gait velocities of slow, normal, and fast. [Methods] A foot pressure analyzer was used to measure changes in foot pressure. [Results] Compared to the normal subjects, the foot pressure of the flatfoot subjects showed a significant increase in the 2–3rd metatarsal region with increasing gait speed, whereas there were significant decreases in the 1st toe and 1st metatarsal regions with increasing gait speed. [Conclusion] The body weight of adults with flatfoot was concentrated on the 2–3rd metatarsal region during the stance phase and increased with walking speed on the ascending slope due to weakening of function of the medial longitudinal arch. PMID:26834348

  10. Description of the difference in plaque index between normal and autistic children age 6-12 years

    Directory of Open Access Journals (Sweden)

    Arinda M Nadya

    2013-07-01

    Full Text Available Background: Oral hygiene status can be seen from the plaque index contained in the mouth. Children aged 6-12 years are school age, they can be taught about oral health care independently. The research objective was to obtain data about description of the differences in plaque index between normal and autistic children aged 6-12 years in Yayasan Autisme Ourdream, Lembaga Pendidikan Autisme Prananda, and SDN Banjarsari in the Bandung city. Materials and Methods: This study was a descriptive analytic. The population was all the students at SDN Banjarsari and autistic children aged 6-12 years in both the foundation. Samplings were collected by simple random sampling, the number of samples obtained normal and autistic children each were 21 persons. Clinical examinations for plaque index using Patient Hygiene Performance (PHP index of Podshadley and Halley. The data were analyzed with independent t-test. Results: The results showed an average of plaque index normal and autistic children aged 6-12 years was 2.06 with a standard deviation of 0.573 and 3.23 with a standard deviation of 0.473. C onclusion: The conclusions showed that there were significant differences in plaque index between normal and autistic children, these values are equally moderate category by Patient Hygiene Performance (PHP index of Podshadley and Halley.

  11. Vestibular evoked myogenic potential eliciting in normal subjects: comparison of four different methods.

    Science.gov (United States)

    Eleftheriadou, Anna; Deftereos, Spyros N; Zarikas, Vasilios; Panagopoulos, Grigoris; Korres, Stavros; Sfetsos, Sotirios; Karageorgiou, Klimentini L; Ferekidou, Elisa; Kandiloros, Dimitrios

    2008-10-01

    Vestibular evoked myogenic potential (VEMP) recording is a new method for testing the otolith receptors and vestibulospinal pathways. The aim of this study was to evaluate the characteristics of VEMP using four different techniques to find reasons to prefer one type of recording over the others. Twenty healthy persons, 10 males and 10 females with ages ranging from 20 to 57 years (mean age 41 years), were enrolled in this study. Eliciting of VEMPs by using monaural or binaural acoustic stimulation and unilateral or bilateral SCM contraction was evaluated; 105 dB NHL acoustic stimulation consisting of 145 dB rarefaction clicks was applied. Latencies of p13, n23, n34, p44 peaks; amplitudes p13-n23 and n34-p44; and interaural amplitude differences (IADs) were assessed. All four methods elicited constant and evident waveforms. The reliability coefficients of amplitudes were high for all four methods and for both waves. However, the higher scores of reliability appeared for the monaural-ipsilateral recording. The results indicated no statistically significant difference between the right and left sides for all four types of VEMP eliciting. No correlation was found between IAD13-23 and IAD34-44 for all four methods. Statistically significant differences were found only for n23 latency among the four methods. Although no evidence to reject or strongly favour a specific method was found, the monaural-ipsilateral recording was associated with some advantages.

  12. Quantitative and qualitative differences in protein expression between papillary thyroid carcinoma and normal thyroid tissue.

    NARCIS (Netherlands)

    Brown, L.M.; Helmke, S.M.; Hunsucker, S.W.; Netea-Maier, R.T.; Chiang, S.A.; Heinz, D.E.; Shroyer, K.R.; Duncan, M.W.; Haugen, B.R.

    2006-01-01

    In order to better understand basic mechanisms of tumor development and identify potential new biomarkers, we have performed difference gel electrophoresis (DIGE) and peptide mass fingerprinting on pooled protein extracts from patients with papillary thyroid carcinoma (PTC) compared with matched nor

  13. A normalized difference vegetation index (NDVI) time-series of idle agriculture lands: A preliminary study

    NARCIS (Netherlands)

    Vaiphasa, C.; Piamduaytham, S.; Vaiphasa, T.; Skidmore, A.K.

    2011-01-01

    In this paper, the NDVI time-series collected from the study area between year 2003 and 2005 of all land cover types are plotted and compared. The study area is the agricultural zones in Banphai District, Khonkean, Thailand. The LANDSAT satellite images of different dates were first transformed into

  14. Computational imaging reveals shape differences between normal and malignant prostates on MRI

    Science.gov (United States)

    Rusu, Mirabela; Purysko, Andrei S.; Verma, Sadhna; Kiechle, Jonathan; Gollamudi, Jay; Ghose, Soumya; Herrmann, Karin; Gulani, Vikas; Paspulati, Raj; Ponsky, Lee; Böhm, Maret; Haynes, Anne-Maree; Moses, Daniel; Shnier, Ron; Delprado, Warick; Thompson, James; Stricker, Phillip; Madabhushi, Anant

    2017-01-01

    We seek to characterize differences in the shape of the prostate and the central gland (combined central and transitional zones) between men with biopsy confirmed prostate cancer and men who were identified as not having prostate cancer either on account of a negative biopsy or had pelvic imaging done for a non-prostate malignancy. T2w MRI from 70 men were acquired at three institutions. The cancer positive group (PCa+) comprised 35 biopsy positive (Bx+) subjects from three institutions (Gleason scores: 6–9, Stage: T1–T3). The negative group (PCa−) combined 24 biopsy negative (Bx−) from two institutions and 11 subjects diagnosed with rectal cancer but with no clinical or MRI indications of prostate cancer (Cl−). The boundaries of the prostate and central gland were delineated on T2w MRI by two expert raters and were used to construct statistical shape atlases for the PCa+, Bx− and Cl− prostates. An atlas comparison was performed via per-voxel statistical tests to localize shape differences (significance assessed at p < 0.05). The atlas comparison revealed central gland hypertrophy in the Bx− subpopulation, resulting in significant volume and posterior side shape differences relative to PCa+ group. Significant differences in the corresponding prostate shapes were noted at the apex when comparing the Cl− and PCa+ prostates. PMID:28145532

  15. Effect of different protein types on second meal postprandial glycaemia in normal weight and normoglycemic subjects.

    Science.gov (United States)

    Silva Ton, Winder Tadeu; das Graças de Almeida, Crislaine; de Morais Cardoso, Leandro; Marvila Girondoli, Yassana; Feliciano Pereira, Patrícia; Viana Gomes Schitini, Josiane Keila; Galvão Cândido, Flávia; Marques Arbex, Priscila; de Cássia Gonçalves Alfenas, Rita

    2014-03-01

    Introducción: La diabetes mellitus es una enfermedad epidémica que afecta a 346 millones de personas en el mundo. El control glicémico es la clave para la prevención y el control de la diabetes. Algunas proteínas pueden estimular la liberación de insulina y modular la respuesta glicémica. Objetivos: Evaluar el efecto del consumo de diferentes tipos de proteínas (proteína de suero de leche, proteína de soja y la clara de huevo) de la glicemia postprandial en una segunda comida en individuos de peso normal y normoglicémicos. Metodología: Este fue un ensayo clínico aleatorizado cruzado. Después de un ayuno nocturno de 12 horas, diez individuos asistieron al laboratorio para beber uno de los batidos de proteínas (suero de leche, soja o clara de huevo) o la bebida control. Treinta minutos más tarde, los individuos consumieron una solución de glucosa (25 g de glucosa). La respuesta glicémica fue monitorizada en los tiempos 0 (antes de solución de glucosa) y 15, 30, 45, 60, 90 y 120 min (después del consumo de la solución de glucosa). El área incrementada bajo la curva de glicemia (iAUC) fue calculada por el método trapezoidal. Por otra parte, la respuesta glicémica se evaluó mediante un nuevo método que utiliza la ecuación de iG. Resultados: En comparación con el control, las bebidas de suero de leche y de proteína de soja reducen iAUC postprandial en 56,5% (p = 0,004) y 44,4% (p = 0,029), respectivamente. La proteína de suero es la única proteína capaz de evitar grandes fluctuaciones y un pico de glicemia postprandial. La evaluación de la respuesta glicémica por la ecuación iG mostró correlación positiva con iAUC (Pearson 0,985, p glucosa resultó en menor iAUC en comparación con la bebida control. La proteína del suero mantiene la glucemia postprandial más estable.

  16. Comparison of Different Levels of Reading Comprehension between Hearing-Impaired Loss and Normal-Hearing Students

    Directory of Open Access Journals (Sweden)

    Azam Sharifi

    2011-12-01

    Full Text Available Background and Aim: Reading skill is one of the most important necessities of students' learning in everyday life. This skill is referred to the ability of comprehension, comment and conclusion from texts and receiving the meaning of the massage which is composed. Educational development in any student has a direct relation with the ability of the comprehension. This study is designed to investigate the effects of hearing loss on reading comprehension in hearing-impaired students compared to normal-hearing ones.Methods: Seventeen hearing-impaired students in 4th year of primary exceptional schools in Karaj, Robatkarim and Shahriyar, Iran, were enrolled in this cross-sectional study. Seventeen normal-hearing students were randomly selected from ordinary schools next to exceptional ones as control group. They were compared for different levels of reading comprehension using the international standard booklet (PIRLS 2001. Results: There was a significant difference in performance between hearing-impaired and normal- hearing students in different levels of reading comprehension (p<0.05.Conclusion: Hearing loss has negative effects on different levels of reading comprehension, so in exceptional centers, reconsideration in educational planning in order to direct education from memorizing to comprehension and deeper layers of learning seems necessary.

  17. Long thermal interactions of PAW with normal tooth structure and different dental biomaterials

    Science.gov (United States)

    Bostǎnaru, Andra-Cristina; Hnatiuc, Eugen; Roşca, Irina; Vasiliu, Ana Lavinia; Doroftei, Mirela; Ursu, Laura; Ailincǎi, Luminiţa Iuliana; Nǎstasǎ, Valentin; Mareş, Mihai

    2016-12-01

    Plasma activated water (PAW) has been widely considered to be an effective method for decontamination. Recently, numerous studies report that plasma-activated water (PAW) also has antibacterial ability to prevent or treat dental caries and periodontal related diseases. In this context, this study presents the first report to evaluate the plasma activated water effect on vital teeth enamel and different dental biomaterials. In this context, this study presents the first report to evaluate long thermal interactions of plasma activated water effect on vital teeth enamel and different dental biomaterials without organic substrate. The results suggest that the long-thermal of treatment with PAW of enamel without organic substrate can dissolve the apatite crystallites which are highly organized hierarchical structures.

  18. Vegetation extraction from high-resolution satellite imagery using the Normalized Difference Vegetation Index (NDVI)

    Science.gov (United States)

    AlShamsi, Meera R.

    2016-10-01

    Over the past years, there has been various urban development all over the UAE. Dubai is one of the cities that experienced rapid growth in both development and population. That growth can have a negative effect on the surrounding environment. Hence, there has been a necessity to protect the environment from these fast pace changes. One of the major impacts this growth can have is on vegetation. As technology is evolving day by day, there is a possibility to monitor changes that are happening on different areas in the world using satellite imagery. The data from these imageries can be utilized to identify vegetation in different areas of an image through a process called vegetation detection. Being able to detect and monitor vegetation is very beneficial for municipal planning and management, and environment authorities. Through this, analysts can monitor vegetation growth in various areas and analyze these changes. By utilizing satellite imagery with the necessary data, different types of vegetation can be studied and analyzed, such as parks, farms, and artificial grass in sports fields. In this paper, vegetation features are detected and extracted through SAFIY system (i.e. the Smart Application for Feature extraction and 3D modeling using high resolution satellite ImagerY) by using high-resolution satellite imagery from DubaiSat-2 and DEIMOS-2 satellites, which provide panchromatic images of 1m resolution and spectral bands (red, green, blue and near infrared) of 4m resolution. SAFIY system is a joint collaboration between MBRSC and DEIMOS Space UK. It uses image-processing algorithms to extract different features (roads, water, vegetation, and buildings) to generate vector maps data. The process to extract green areas (vegetation) utilize spectral information (such as, the red and near infrared bands) from the satellite images. These detected vegetation features will be extracted as vector data in SAFIY system and can be updated and edited by end-users, such as

  19. Effect of different types of music on exercise performance in normal individuals.

    Science.gov (United States)

    Thakur, Anuprita M; Yardi, Sujata S

    2013-01-01

    While exercising, people seem to enjoy listening to music believing that it relaxes them or helps give the necessary rhythm for exercise. But is music really beneficial? In view of different people listening to different types of music, this study was intended to assess effect of different types of music on exercise performance. 30 healthy female college students in the age group of 18 to 25 years were made to walk on the treadmill 3 times at one week interval: without music (A), with slow music (B), with fast music (C). Duration of exercise and rate of perceived exertion were recorded at the end of each session. The results showed an increase in the duration of exercise in Group B and Group C as compared to Group A and the increase was more in Group C as compared to Group B. It was observed that level of RPE was the same at the end of every exercise session. The reason for increase in exercise duration with music could be because of various factors like dissociation, arousal, motivation, etc. It can be thus suggested that exercises can be performed for longer duration with music than without music and the effect is more with fast music than with slow music. Also with music, the same level of exertion is perceived though the walking duration is considerably increased.

  20. Moralizing, normalizing or absent teachers – how children’s fiction represents differences

    Directory of Open Access Journals (Sweden)

    Edgar Roberto Kirchof

    2008-12-01

    Full Text Available Due to a increase of children’s fiction addressing the subject of ‘differences’ in the last few years in the Brazilian context, this study proposes a reflection on the way these books represent differences related to minority groups and gender. The analyses are focused on the character roles that are played by the teacher as she/he is faced with differences. Ten books published in Brazil by different authors and publishing houses between 2004 and 2008 were analyzed. The main argument of the analyses is based on the assumption that the increase of the issue of ‘difference’ in recent Brazilian children’s books is in accordance with various state policies relating to minorities rights, on the one hand, and to a broad development of criticism of children’s fiction from the 1970s until the present day as to stereotypical representation of gender and minority groups in the British and North-American context, on the other hand.

  1. Sound localization, sound lateralization, and binaural masking level differences in young children with normal hearing.

    Science.gov (United States)

    Van Deun, Lieselot; van Wieringen, Astrid; Van den Bogaert, Tim; Scherf, Fanny; Offeciers, F Erwin; Van de Heyning, Paul H; Desloovere, Christian; Dhooge, Ingeborg J; Deggouj, Naïma; De Raeve, Leo; Wouters, Jan

    2009-04-01

    In this study, procedures for measuring sound localization, sound lateralization, and binaural masking level differences (BMLDs) in young children were developed. Sensitivity for these tasks was assessed in large groups of children between 4 and 9 yr of age to investigate potential developmental trends. Sound localization was measured in the sound field, with a broadband bell-ring presented from one of nine loudspeakers positioned in the frontal horizontal field. A group of 33 children between 4 and 6 yr of age and 5 adults took part in this experiment. Sound lateralization based on interaural time differences was measured with headphones in 49 children between 4 and 9 yr of age and 10 adults. A low-frequency stimulus containing harmonics 2 to 5 from a click train with a rate of 160 Hz was used. In the BMLD test, the same filtered click train was presented diotically or dichotically (phase reversed or time delayed) in a broadband (200 to 1000 Hz) frozen noise to 23 children between 4 and 6 yr of age and 10 adults. For comparison with literature, additional measurements with a 500-Hz sinusoid were administered to adults. All tasks were adapted to the interest and attention span of young children. Children of 5 yr of age did not perform significantly different from adults on the sound localization task, but mean absolute errors were larger for the 4-yr-olds. Also on the BMLD task, 5-yr-old children performed at the adult level, whereas the 4-yr-old children obtained significantly less binaural unmasking compared with the adults. Concerning sound lateralization, a small but significant difference between adults and children existed, but no age effects were apparent in the 4- to 9-yr-old group. Overall, the variation was relatively large in the 4-yr-old group, with some of the children performing at adult level, in all three tasks. The results of this study show that the modified procedures are suitable for testing children from the age of 4 to 5 yr. Furthermore, it

  2. [Correlation analysis on normalized difference vegetation index (NDVI) of different vegetations and climatic factors in Southwest China].

    Science.gov (United States)

    Zhang, Yuan-Dong; Zhang, Xiao-He; Liu, Shi-Rong

    2011-02-01

    Based on the 1982-2006 NDVI remote sensing data and meteorological data of Southwest China, and by using GIS technology, this paper interpolated and extracted the mean annual temperature, annual precipitation, and drought index in the region, and analyzed the correlations of the annual variation of NDVI in different vegetation types (marsh, shrub, bush, grassland, meadow, coniferous forest, broad-leaved forest, alpine vegetation, and cultural vegetation) with corresponding climatic factors. In 1982-2006, the NDVI, mean annual temperature, and annual precipitation had an overall increasing trend, and the drought index decreased. Particularly, the upward trend of mean annual temperature was statistically significant. Among the nine vegetation types, the NDVI of bush and mash decreased, and the downward trend was significant for bush. The NDVI of the other seven vegetation types increased, and the upward trend was significant for coniferous forest, meadow, and alpine vegetation, and extremely significant for shrub. The mean annual temperature in the areas with all the nine vegetation types increased significantly, while the annual precipitation had no significant change. The drought index in the areas with marsh, bush, and cultural vegetation presented an increasing trend, that in the areas with meadow and alpine vegetation decreased significantly, and this index in the areas with other four vegetation types had an unobvious decreasing trend. The NDVI of shrub and coniferous forest had a significantly positive correlation with mean annual temperature, and that of shrub and meadow had significantly negative correlation with drought index. Under the conditions of the other two climatic factors unchanged, the NDVI of coniferous forest, broad-leaved forest, and alpine vegetation showed the strongest correlation with mean annual temperature, that of grass showed the strongest correlation with annual precipitation, and the NDVI of mash, shrub, grass, meadow, and cultural

  3. Explicit Construction of Self-Dual Integral Normal Bases for the Square-Root of the Inverse Different

    CERN Document Server

    Pickett, Erik Jarl

    2010-01-01

    Let $K$ be a finite extension of $\\Q_p$, let $L/K$ be a finite abelian Galois extension of odd degree and let $\\bo_L$ be the valuation ring of $L$. We define $A_{L/K}$ to be the unique fractional $\\bo_L$-ideal with square equal to the inverse different of $L/K$. For $p$ an odd prime and $L/\\Q_p$ contained in certain cyclotomic extensions, Erez has described integral normal bases for $A_{L/\\Q_p}$ that are self-dual with respect to the trace form. Assuming $K/\\Q_p$ to be unramified we generate odd abelian weakly ramified extensions of $K$ using Lubin-Tate formal groups. We then use Dwork's exponential power series to explicitly construct self-dual integral normal bases for the square-root of the inverse different in these extensions.

  4. Sumatriptan does not affect arteriovenous oxygen differences in jugular and cubital veins in normal human subjects

    DEFF Research Database (Denmark)

    Wienecke, T.; Hansen, J.M.; Petersen, J.;

    2008-01-01

    Arteriovenous anastomoses (AVAs) may open up during migraine attacks. In studies with anaesthetized and bilaterally vagosympatectomized pigs, triptans reduce AVA blood flow and increase the arteriovenous O-2 difference (AVDO(2)). To investigate whether subcutaneous sumatriptan 6 mg could induce...... changes in the AVDO(2), we measured the AVDO(2) in the external jugular vein in healthy subjects. We also measured the AVDO(2) in the internal jugular and cubital veins. There were no changes in AVDO(2) after subcutaneous sumatriptan, probably because AVA blood flow is limited in humans with an intact...

  5. Comparison of different normalization strategies for the analysis of glomerular microRNAs in IgA nephropathy.

    Science.gov (United States)

    Bockmeyer, Clemens L; Säuberlich, Karen; Wittig, Juliane; Eßer, Marc; Roeder, Sebastian S; Vester, Udo; Hoyer, Peter F; Agustian, Putri A; Zeuschner, Philip; Amann, Kerstin; Daniel, Christoph; Becker, Jan U

    2016-08-24

    Small nucleolar RNAs (snoRNAs) have been used for normalization in glomerular microRNA (miRNA) quantification without confirmation of validity. Our aim was to identify glomerular reference miRNAs in IgA nephropathy. We compared miRNAs in human paraffin-embedded renal biopsies from patients with cellular-crescentic IgA-GN (n = 5; crescentic IgA-GN) and non-crescentic IgA-GN (n = 5; IgA-GN) to mild interstitial nephritis without glomerular abnormalities (controls, n = 5). Laser-microdissected glomeruli were used for expression profiling of 762 miRNAs by low-density TaqMan arrays (cards A and B). The comparison of different normalization methods (GeNormPlus, NormFinder, global mean and snoRNAs) in crescentic IgA-GN, IgA-GN and controls yielded similar results. However, levels of significance and the range of relative expression differed. In median, two normalization methods demonstrated similar results. GeNormPlus and NormFinder gave different top ranked reference miRNAs. Stability ranking for snoRNAs varied between cards A and B. In conclusion, we suggest the geometric mean of the most stable reference miRNAs found in GeNormPlus (miR-26b-5p), NormFinder (miR-28-5p) and snoRNAs (RNU44) as reference. It should be considered that significant differences could be missed using one particular normalization method. As a starting point for glomerular miRNA studies in IgA nephropathy we provide a library of miRNAs.

  6. Transcription profiles of Streptococcus pneumoniae grown under different conditions of normal gravitation

    Science.gov (United States)

    Allen, C. A.; Galindo, C. L.; Pandya, U.; Watson, D. A.; Chopra, A. K.; Niesel, D. W.

    2007-02-01

    High-aspect rotating vessels (HARVs) are used to study the effects low-shear modeled microgravity (LSMMG) on bacterial gene expression. LSMMG is generated by orienting HARVs with the axis of rotation perpendicular to the gravity vector while gravitational controls are oriented with the axis of rotation parallel to the gravity vector. Microarray analysis was performed on Streptococcus pneumoniae TIGR4 grown in HARVs under three conditions (LSMMG, 1×g, and static) to determine if global transcriptional activity is altered between different gravitational controls and LSMMG. Results revealed 101 differentially expressed genes under static conditions compared to 1×g, 46 genes between 1×g and LSMMG, and nine genes between static and LSMMG. Hierarchical cluster analysis revealed 15 genes exhibiting similar expression patterns under static conditions compared to 1×g. These results indicate that rotation, in addition to low-shear forces, might contribute to bacterial adaptation to the LSMMG.

  7. Base excision repair activities differ in human lung cancer cells and corresponding normal controls

    DEFF Research Database (Denmark)

    Karahalil, Bensu; Bohr, Vilhelm A; De Souza-Pinto, Nadja C

    2010-01-01

    Oxidative damage to DNA is thought to play a role in carcinogenesis by causing mutations, and indeed accumulation of oxidized DNA bases has been observed in samples obtained from tumors but not from surrounding tissue within the same patient. Base excision repair (BER) is the main pathway...... for the repair of oxidized modifications both in nuclear and mitochondrial DNA. In order to ascertain whether diminished BER capacity might account for increased levels of oxidative DNA damage in cancer cells, the activities of BER enzymes in three different lung cancer cell lines and their non......-cancerous counterparts were measured using oligonucleotide substrates with single DNA lesions to assess specific BER enzymes. The activities of four BER enzymes, OGG1, NTH1, UDG and APE1, were compared in mitochondrial and nuclear extracts. For each specific lesion, the repair activities were similar among the three...

  8. Differences in assertive speech acts produced by children with autism, Asperger syndrome, specific language impairment, and normal development.

    Science.gov (United States)

    Ziatas, Kathryn; Durkin, Kevin; Pratt, Chris

    2003-01-01

    The assertive speech acts of children with autism (n = 12) and Asperger syndrome (n = 12), individually matched to children with specific language impairment (SLI; n = 24) and children with normal development (n = 24) were studied in the context of gently structured conversation. These children also completed the false belief test of theory of mind. The children with autism used significantly lower proportions of assertions involving explanations and descriptions than the children with SLI or normal development and significantly lower proportions of assertions involving internal state and explanations than the children with Asperger syndrome. The children with autism used a higher proportion of assertions involving identifications than any other group. The assertions of the children with Asperger syndrome were generally not different than those of the children with SLI or normal development except for a higher proportion of assertions involving own internal state. Further analysis of the mental assertions revealed that the children with autism and Asperger syndrome predominantly referred to desire and made few references to thought and belief, whereas the children with SLI and those with normal development used a higher proportion of references to thought and belief.

  9. Gene Expression Differences between Enriched Normal and Chronic Myelogenous Leukemia Quiescent Stem/Progenitor Cells and Correlations with Biological Abnormalities

    Directory of Open Access Journals (Sweden)

    M. Affer

    2011-01-01

    Full Text Available In comparing gene expression of normal and CML CD34+ quiescent (G0 cell, 292 genes were downregulated and 192 genes upregulated in the CML/G0 Cells. The differentially expressed genes were grouped according to their reported functions, and correlations were sought with biological differences previously observed between the same groups. The most relevant findings include the following. (i CML G0 cells are in a more advanced stage of development and more poised to proliferate than normal G0 cells. (ii When CML G0 cells are stimulated to proliferate, they differentiate and mature more rapidly than normal counterpart. (iii Whereas normal G0 cells form only granulocyte/monocyte colonies when stimulated by cytokines, CML G0 cells form a combination of the above and erythroid clusters and colonies. (iv Prominin-1 is the gene most downregulated in CML G0 cells, and this appears to be associated with the spontaneous formation of erythroid colonies by CML progenitors without EPO.

  10. Differences in center of pressure trajectory between normal and steppage gait

    Directory of Open Access Journals (Sweden)

    Nima Jamshidi

    2010-01-01

    Full Text Available Background: This pilot study aimed to assess the differences in center of pressure trajectory in neuropathic patients with steppage gait. Steppage gait has previously been evaluated by several biomechanical methods, but plantar pressure distribution has been much less studied. The purpose of this study was to analyze the changes in center of pressure tra-jectory using a force plate. Methods: The steppage gait group was selected from the patients using drop foot brace (25 male and the control group was selected from Isfahan university students (20 male. They walked at self- selected speed at a mean of ten tri-als (+2 to collect the center of pressure using a force plate. Center of pressure patterns were categorized into four pat-terns based on the center of pressure displacement magnitude (spatial features through time (temporal features when the longitudinal axis of the insole was plotted as the Y- axis and the transverse axis of the insole as X- axis during stance phase. Results: The horizontal angle measured from center of pressure linear regression was positive in the control group (4.6 ± 2.4 (p < 0.005, but negative in the patient group (- 2.3 ± 1.6 (p < 0.005. Conclusions: The finding of this research measured center of pressure trajectory in steppage gait over time, which is useful for designing better shoe sole and also orthopaedic device and better understanding of stability in patients with drop foot.

  11. Differences in Centre of Pressure Trajectory between Normal and Steppage Gait

    Directory of Open Access Journals (Sweden)

    Nima Jamshidi

    2010-01-01

    Full Text Available Abstract Background: This pilot study aimed to assess the differences in centre of pressure trajectory in neuropathic patients with steppage gait. Steppage gait was previously evaluated by several biomechanical methods, but plantar pressure distribution has been much less studied. Our purpose was to analyze the changes in centre of pressure trajectory using a force plate. Methods: The steppage gait group was selected from the patients using drop foot brace (25 male and the control group was selected from Isfahan university students (20 male. They walked at self-selected at a mean of ten trials (+2 to collect the centre of pressure using a force plate. Centre of pressure patterns were categorized into four patterns based on the centre of pressure displacement magnitude (spatial features through time (temporal features when the longitudinal axis of the insole was plotted as the Y-axis and the transverse axis of the insole as X-axis during stance phase. Results: The result of this research indicated that the horizontal angle measured from centre of pressure linear regression in control group is positive (4.6± 2.4 (p<0.005 but in patients group is negative (-2.3±1.6 (p<0.005. Conclusions: The finding of this research measured centre of pressure trajectory in steppage gait over time which is useful for designing better shoe sole and also orthopaedic device and better understanding of stability in patients with drop foot.

  12. Sex-differences in grey-white matter structure in normal-reading and dyslexic adolescents.

    Science.gov (United States)

    Sandu, Anca-Larisa; Specht, Karsten; Beneventi, Harald; Lundervold, Arvid; Hugdahl, Kenneth

    2008-06-13

    MR images were used to look for brain structure irregularities in adolescent children with dyslexia by use of combined grey and white matter volume measurements and fractal dimension (FD) of the grey-white matter border. The data were collected from 13 dyslexic adolescent (8 boys and 5 girls) that were compared with 18 control subjects (8 boys and 10 girls). The MR images were first segmented, and the volume as well as the FD of the grey/white matter border for the whole brain and for each hemisphere was computed. Changes were found in the measured volumes of both grey and white matter and were best reflected in the ratio of grey/white matter and in FD values, especially in the left hemisphere. The results showed that, although dyslexia is less frequent in women, the structural differences in the brain are more pronounced in their case, pointing to an increased vulnerability of the female brain to morphological changes associated with dyslexia.

  13. Determination of Leaf Area Index, Total Foliar N, and Normalized Difference Vegetation Index for Arctic Ecosystems Dominated by Cassiope tetragona

    DEFF Research Database (Denmark)

    Campioli, M; Street, LE; Michelsen, Anders

    2009-01-01

    have not been accurately quantified. We address this knowledge gap by (i) direct measurements of LAI and TFN for C. tetragona, and (ii) determining TFN-LAI and LAI–normalized difference vegetation index (NDVI) relationships for typical C. tetragona tundras in the subarctic (Sweden) and High Arctic...... leaf N and biomass. The LAI-NDVI and TFN-LAI relationships showed high correlation and can be used to estimate indirectly LAI and TFN. The LAI-NDVI relationship for C. tetragona vegetation differed from a generic LAI-NDVI relationship for arctic tundra, whereas the TFN-LAI relationship did not. Overall...

  14. Endogenous pancreatic polypeptide in different vascular beds: relationship to release and degradation in subjects with normal and decreased kidney function

    DEFF Research Database (Denmark)

    Henriksen, Jens Henrik Sahl; Schwartz, T W; Bülow, J B

    1986-01-01

    The plasma concentration of pancreatic polypeptide (PP-like immunoreactivity) was measured in different vascular beds in order to determine regional kinetics of endogenous PP in fasting, supine subjects with normal or moderately decreased kidney function. Patients with kidney disease (n = 10) had...... a progressively increasing rate of PP secretion in subjects with raised circulating PP. No statistically significant difference could be detected between systemic and renal venous PP or across the lung, left adrenal gland, or lower limb. Assuming steady state between secretion and biodegradation, the metabolic...

  15. What pediatricians should know about normal language development: ensuring cultural differences are not diagnosed as disorders.

    Science.gov (United States)

    Weiss, Amy L; Van Haren, Melissa S

    2003-07-01

    The roles and responsibilities of speech-language pathologists and pediatricians have become greater with the changing population demographics in the United States. In some states, the majority of the population belongs to a national cultural minority, eg, New Mexico. Even a state such as Iowa, with only a 5% nonmajority population, has a school-aged population that is almost 10% nonmajority. This growth of diversity is likely to continue. Rather than viewing sensitivity to the influence of culture on language learning and other developmental areas as an "add-on" to a practice, it may be wiser to recognize that approaching all clients with as few assumptions about their behaviors as possible will guarantee nonbiased service delivery for all. Without nonbiased service delivery, incorrect diagnoses and provision of inappropriate therapy become more likely. Fortunately, many resources are available to assist pediatricians and speech-language pathologists in learning about various cultures. Institutional review boards have become more vigilant about the inclusion of a cross-section of subject populations as participants in research studies in addition to protecting the rights of all participants. Funding agencies also have expressed as a priority the inclusion of research subjects from minority populations to add to the information available about the incidence and prevalence of disorders across the range of our potential patients. In a society in which cultural differences are not just defined by race or ethnicity, but by gender, sexual orientation, age, geographic region, and religion, belief systems about disease, disability, and treatment are dynamic entities for health professionals to take into consideration. It is a challenge that speech-language pathologists and pediatricians must meet if they are to provide the best and most appropriate services for their patients.

  16. Normal and tumour cervical cells respond differently to vaginal lactobacilli, independent of pH and lactate.

    Science.gov (United States)

    Motevaseli, Elahe; Shirzad, Mahdieh; Akrami, Seyed Mohammad; Mousavi, Azam-Sadat; Mirsalehian, Akbar; Modarressi, Mohammad Hossein

    2013-07-01

    Cervical cancer is a human papilloma virus (HPV)-related cancer, but most HPV infections are transient or intermittent and resolve spontaneously. Thus, other factors, such as cervical microflora, which are dominated by lactobacilli, must be involved in invasive cervical carcinoma development after HPV infection. Previous studies have demonstrated that lactobacilli have antitumour effects, and it is possible that vaginal lactobacilli prevent cervical cancer. Here we examined the proliferative and apoptotic responses of normal and tumour cervical cells to common vaginal lactobacilli components by investigating human normal fibroblast-like cervical (normal cervical) and HeLa (cervical tumour) cell responses to Lactobacillus gasseri and Lactobacillus crispatus. The effects of different lactobacilli components, such as culture supernatants, cytoplasmic extracts, cell-wall extracts and live cells, were determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, trypan blue staining, lactate dehydrogenase assay and colorimetric caspase-3 activity assay. Changes in caspase-3 and human chorionic gonadotropin β (hCGβ) expression were analysed by quantitative RT-PCR. Tumour cell growth inhibition by culture supernatants was higher than that by pH- and lactate-adjusted controls. However, the effects of the supernatants on normal cells were similar to those of lactate-adjusted controls. Apoptosis was inhibited by supernatants, which was consistent with higher hCGβ expression since hCG inhibits apoptosis. Our study demonstrated that common vaginal lactobacilli exert cytotoxic effects on cervical tumour cells, but not on normal cells, and that this cytotoxicity is independent of pH and lactate. Our results encourage further studies on the interaction between lactobacilli and cervical cells, and administration of common vaginal lactobacilli as probiotics.

  17. Different Kinetics of Puerarin in Plasma of Normal and Depressed Rats After Oral Administration of Chinese Medicine TZ18

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The objective of this study is to quantify the puerarin in rat plasma following oral administration of TZ18 and compare the pharmacokinetics characteristics of puerarin in normal rats with that in depression model rats. A high performance liquid chromatography method was used to quantify the puerarin due to its Intra- and inter-day precision coefficients of variation and accuracy bias were acceptable (Maximum coefficient of variation was 5.74% for intra-day and 3.09% for inter-day) over the entire range. The recoveries spectively. The concentration-time curves for both normal rats and depression model rats were fit to a twocompartment model with the first order absorption. The results show significant differences in the main pharmacokinetic parameters of peak time, peak concentration, and the area under the concentration-time curve between the two kinds of rats.

  18. Uncaria tomentosa Leaves Decoction Modulates Differently ROS Production in Cancer and Normal Cells, and Effects Cisplatin Cytotoxicity.

    Science.gov (United States)

    Kośmider, Anita; Czepielewska, Edyta; Kuraś, Mieczysław; Gulewicz, Krzysztof; Pietrzak, Wioleta; Nowak, Renata; Nowicka, Grażyna

    2017-04-12

    Uncaria tomentosa is a woody vine with a long history of use in traditional Peruvian medicine and nowadays supplements containing this vine as ingredient are available. Immunomodulating, anti-inflammatory and anticancer properties of Uncaria tomentosa have been suggested and attributed mainly to the presence of tetracyclic or pentacyclic oxindole alkaloids. However, the synergic action of different compounds occurring in extracts and modulation of redox processes may significantly influence the anticancer activity of Uncaria tomentosa. The aim of the present study was to investigate for the first time the cytotoxic effects of the tetracyclic alkaloids free aqueous extract (decoction) of dried Uncaria tomentosa leaf blades in normal and cancer cells, and to assess the effect of the tested extract on cisplatin (CDDP) cytotoxicity. Tested Uncaria tomentosa extract was not cytotoxic for NHDF cells, but demonstrated cytotoxic effect against HepG2 cells. The extract increased ROS production in HepG2 cells, which resulted in decreased GSH level, leading to apoptosis of these cells through activation of caspase-3 and caspase-7. A reduction of NF-κB active form was observed in cancer cells. In normal cells the extract did not affect ROS production, GSH level and NF-κB activity, and maintained cell viability. HepG2 cells incubation with Uncaria tomentosa decoction and simultaneously with CDDP resulted in an increase in CDPP cytotoxic activity against HepG2, while under the same conditions Uncaria tomentosa prevents NHDF cell viability reduction due to CDDP. The results indicate that Uncaria tomentosa leaves decoction modulates differently cancer and normal cells oxidative metabolism and, enhanced cytotoxicity of CDDP against cancer cells and at the same time increased normal healthy cells resistance to cisplatin. Further studies are needed to confirm our observations and to describe underlying molecular mechanism, and the potential usefulness of Uncaria tomentosa decoction

  19. Pharmacokinetic difference of berberine between normal and chronic visceral hypersensitivity irritable bowel syndrome rats and its mechanism.

    Science.gov (United States)

    Gong, Zipeng; Chen, Ying; Zhang, Ruijie; Yang, Qing; Wang, Yajie; Guo, Yan; Zhou, Bingbing; Weng, Xiaogang; Liu, Xuchen; Li, Yujie; Zhu, Xiaoxin; Dong, Yu

    2015-10-01

    Berberine is one of active alkaloids from Rhizoma coptidis in traditional Chinese medicine. The pharmacokinetics of berberine in rat plasma were compared between normal and chronic visceral hypersensitivity irritable bowel syndrome rats (CVH-IBS) established by mechanical colon irritation using angioplasty balloons for 2 weeks after oral administration of berberine hydrochloride (25 mg/kg) with the equivalent dose of 22 mg/kg for berberine according to body weight. Immunohistochemical analysis of c-fos and myosin light chain kinase (MLCK) and immunofluorescence analysis of MLCK in rat colon were conducted. Quantification of berberine in rat plasma was achieved by using a sensitive and rapid UPLC-MS/MS method. Plasma samples were collected at 15 different points in time and the pharmacokinetic parameters were analyzed by WinNonlin software. The great different pharmacokinetic behavior of berberine was observed between normal and CVH-IBS model rats. Compared with normal group, T1/2 and AUC(0-t) of berberine in the model group were significantly increased, respectively (573.21 ± 127.53 vs 948.22 ± 388.57 min; 8,657.19 ± 1,562.54 vs 11,415.12 ± 1,670.72 min.ng/ml). Cl/F of berberine in the model group significantly decreased, respectively (13.89 ± 1.69 vs 9.19 ± 2.91 L/h/kg). Additionally, the expressions of c-fos and MLCK in model group were higher than those in normal group. The pharmacokinetic behavior of berberine was significantly altered in CVH-IBS pathological conditions, which indicated the dosage modification of berberine hydrochloride in CVH-IBS were necessary. Especially, improved exposure to berberine in rat plasma in CVH-IBS model rats was attributed to increased the expression of MLCK.

  20. Land Surface Temperature Retrieval in Wetlands Using Normalized Difference Vegetation Index-Emissivity Estimation and ASTER Emissivity Product

    Science.gov (United States)

    Muro, Javier; Heinmann, Sascha; Strauch, Adrian; Menz, Gunter

    2016-08-01

    Land Surface Temperature (LST) has the potential to act as a continuous indicator of the ecological status of wetlands. Accurate emissivity values are required in order to calculate precise LST. We test two emissivity retrieval methods and their influence on LST calculated from a Landsat 7 image of a highly dynamic wetland in Southern Spain. LST calculated using NDVI (Normalized Difference Vegetation Index) threshold estimations and the ASTER emissivity product are compared. The results show differences of around 0-1 K for most land covers, and up to 3 K for areas of bare soil when Landsat and ASTER images have the same acquisition date. Tests using Landsat and ASTER images from different seasons do not show greater differences between both LSTs. This has important implications for automated LST retrieval methods, such as the one planed by the USGS using Landsat and ASTER emissivity products.

  1. Assessing Differences in Risk Perceptions About Obesity Among "Normal-Weight" and "Overweight" Adolescents--A Qualitative Study.

    Science.gov (United States)

    Gavaravarapu, SubbaRao M; Rao, K Mallikarjuna; Nagalla, Balakrishna; Avula, Laxmaiah

    2015-01-01

    To assess the differences in risk perceptions of overweight/obese and normal-weight adolescents about obesity and associated risk factors. Qualitative study using focus group discussions (FGDs). Five randomly selected schools from the South Indian city of Hyderabad. Seventy-nine adolescents (ages 11-14 years) participated in 10 FGDs (5 each with overweight/obese and normal-weight groups). Whether obesity-related risk perceptions differ with actual weight status or not. FGDs were recorded, transcribed, and manually coded for thematic analysis. Results were presented according to 6 themes. At each stage of coding and analysis, reports were read independently by 2-3 researchers and the inter-coder reliability was high (ratio of number of agreements against the sum of agreements plus disagreements was over 90%). Adolescents across the groups had limited understanding of nutrition during adolescence as well as causes and consequences of obesity. The optimistic bias that they were less vulnerable compared to others to the risks of obesity was evident from perceptions of overweight groups. While overweight adolescents argued that obesity was hereditary, the normal-weight participants perceived "faulty food habits" and laziness as the reasons. Adolescents across the groups considered fruits and vegetables as healthy foods. There were clear differences in perceptions of adolescents of different weight status. Employing the risk perception analysis framework, this study identified the following adolescent traits: responsive, avoidance, and indifference, which may be useful for developing nutrition communication programs. Copyright © 2015 Society for Nutrition Education and Behavior. Published by Elsevier Inc. All rights reserved.

  2. Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR

    Directory of Open Access Journals (Sweden)

    Deforce Dieter

    2006-08-01

    Full Text Available Abstract Background Candida albicans biofilms are commonly found on indwelling medical devices. However, the molecular basis of biofilm formation and development is not completely understood. Expression analysis of genes potentially involved in these processes, such as the ALS (Agglutinine Like Sequence gene family can be performed using quantitative PCR (qPCR. In the present study, we investigated the expression stability of eight housekeeping genes potentially useful as reference genes to study gene expression in Candida albicans (C. albicans biofilms, using the geNorm Visual Basic Application (VBA for Microsoft Excel. To validate our normalization strategies we determined differences in ALS1 and ALS3 expression levels between C. albicans biofilm cells and their planktonic counterparts. Results The eight genes tested in this study are ranked according to their expression stability (from most stable to least stable as follows: ACT1 (β-actin/PMA1 (adenosine triphosphatase, RIP (ubiquinol cytochrome-c reductase complex component, RPP2B (cytosolic ribosomal acidic protein P2B, LSC2 (succinyl-CoA synthetase β-subunit fragment, IMH3 (inosine-5'-monophosphate dehydrogenase fragment, CPA1 (carbamoyl-phosphate synthethase small subunit and GAPDH (glyceraldehyde-3-phosphate dehydrogenase. Our data indicate that five genes are necessary for accurate and reliable normalization of gene expression data in C. albicans biofilms. Using different normalization strategies, we found a significant upregulation of the ALS1 gene and downregulation of the ALS3 gene in C. albicans biofilms grown on silicone disks in a continous flow system, the CDC reactor (Centre for Disease Control, for 24 hours. Conclusion In conclusion, we recommend the use of the geometric mean of the relative expression values from the five housekeeping genes (ACT1, PMA1, RIP, RPP2B and LSC2 for normalization, when analysing differences in gene expression levels between C. albicans biofilm

  3. The circadian variation in Anti-Mullerian hormone in patients with polycystic ovary syndrome differs significantly from normally ovulating women.

    Directory of Open Access Journals (Sweden)

    Leif Bungum

    Full Text Available OBJECTIVE: [corrected] To improve the biologic understanding of the Polycystic Ovarian Syndrome (PCOS condition by examining the circadian variation and relationship between Anti Müllerian Hormone (AMH, gonadotropins and ovarian steroids in PCOS patients compared to normally ovulating and menstruating women. By comparing the pattern of co-variation between AMH and Luteinizing Hormone, two compounds closely linked to hyperandrogenism and anovulation in PCOS, the involvement of the Hypothalamic-Pituitary-Ovarian axis in PCOS pathology could be elucidated. PATIENTS: Eight normal-weighted young, anovulatory PCOS-women as study group and ten normal menstruating and ovulating women as controls. INTERVENTIONS: Observational prospective study of the circadian variation in AMH, gonadotropins, sex steroids and androgens in a study and a control group. A circadian profile was performed in each study and control subject during a 24-h period by blood sampling every second hour, starting at 8:00 a.m. and continuing until 8:00 a.m. the following day. RESULTS: Significant differences in hormonal levels were found between the groups, with higher concentrations of AMH, LH and androgens in the PCOS group and lower amounts of FSH and progesterone. A distinct difference in the circadian variation pattern of AMH and LH between PCOS patients and normal controls was seen, with PCOS patients presenting a uniform pattern in serum levels of AMH and LH throughout the study period, without significant nadir late-night values as was seen in the control group. In PCOS women, a significant positive association between LH/ FSH and testosterone was found opposite to controls. MAIN OUTCOME MEASURES: Circadian variation in Anti-Müllerian Hormone, gonadotropins and ovarian steroids and the covariation between them. CONCLUSION: A significant difference in the circadian secretion of LH and AMH in PCOS women compared to normally ovulating women indicate an increased GnRH pulse

  4. Spatial selective auditory attention in the presence of reverberant energy: individual differences in normal-hearing listeners.

    Science.gov (United States)

    Ruggles, Dorea; Shinn-Cunningham, Barbara

    2011-06-01

    Listeners can selectively attend to a desired target by directing attention to known target source features, such as location or pitch. Reverberation, however, reduces the reliability of the cues that allow a target source to be segregated and selected from a sound mixture. Given this, it is likely that reverberant energy interferes with selective auditory attention. Anecdotal reports suggest that the ability to focus spatial auditory attention degrades even with early aging, yet there is little evidence that middle-aged listeners have behavioral deficits on tasks requiring selective auditory attention. The current study was designed to look for individual differences in selective attention ability and to see if any such differences correlate with age. Normal-hearing adults, ranging in age from 18 to 55 years, were asked to report a stream of digits located directly ahead in a simulated rectangular room. Simultaneous, competing masker digit streams were simulated at locations 15° left and right of center. The level of reverberation was varied to alter task difficulty by interfering with localization cues (increasing localization blur). Overall, performance was best in the anechoic condition and worst in the high-reverberation condition. Listeners nearly always reported a digit from one of the three competing streams, showing that reverberation did not render the digits unintelligible. Importantly, inter-subject differences were extremely large. These differences, however, were not significantly correlated with age, memory span, or hearing status. These results show that listeners with audiometrically normal pure tone thresholds differ in their ability to selectively attend to a desired source, a task important in everyday communication. Further work is necessary to determine if these differences arise from differences in peripheral auditory function or in more central function.

  5. Lactic Acid Bacteria Protects Caenorhabditis elegans from Toxicity of Graphene Oxide by Maintaining Normal Intestinal Permeability under different Genetic Backgrounds

    Science.gov (United States)

    Zhao, Yunli; Yu, Xiaoming; Jia, Ruhan; Yang, Ruilong; Rui, Qi; Wang, Dayong

    2015-11-01

    Lactic acid bacteria (LAB) is safe and useful for food and feed fermentation. We employed Caenorhabditis elegans to investigate the possible beneficial effect of LAB (Lactobacillus bulgaricus) pretreatment against toxicity of graphene oxide (GO) and the underlying mechanisms. LAB prevented GO toxicity on the functions of both primary and secondary targeted organs in wild-type nematodes. LAB blocked translocation of GO into secondary targeted organs through intestinal barrier by maintaining normal intestinal permeability in wild-type nematodes. Moreover, LAB prevented GO damage on the functions of both primary and secondary targeted organs in exposed nematodes with mutations of susceptible genes (sod-2, sod-3, gas-1, and aak-2) to GO toxicity by sustaining normal intestinal permeability. LAB also sustained the normal defecation behavior in both wild-type nematodes and nematodes with mutations of susceptible genes. Therefore, the beneficial role of LAB against GO toxicity under different genetic backgrounds may be due to the combinational effects on intestinal permeability and defecation behavior. Moreover, the beneficial effects of LAB against GO toxicity was dependent on the function of ACS-22, homologous to mammalian FATP4 to mammalian FATP4. Our study provides highlight on establishment of pharmacological strategy to protect intestinal barrier from toxicity of GO.

  6. Differences in Learning Strategies, Goal Orientations, and Self-Concept between Overachieving, Normal-Achieving, and Underachieving Secondary Students.

    Science.gov (United States)

    Castejón, Juan L; Gilar, Raquel; Veas, Alejandro; Miñano, Pablo

    2016-01-01

    The aims of this work were to identify and establish differential characteristics in learning strategies, goal orientations, and self-concept between overachieving, normal-achieving and underachieving secondary students. A total of 1400 Spanish first and second year high school students from the South-East geographical area participated in this study. Three groups of students were established: a group with underachieving students, a group with a normal level of achievement, and a third group with overachieving students. The students were assigned to each group depending on the residual punctuations obtained from a multiple regression analysis in which the punctuation of an IQ test was the predictor and a measure composed of the school grades of nine subjects was the criteria. The results of one-way ANOVA and the Games-Howell post-hoc test showed that underachieving students had significantly lower punctuations in all of the measures of learning strategies and learning goals, as well as all of the academic self-concept, personal self-concept, parental relationship, honesty, and personal stability factors. In contrast, overachieving students had higher punctuations than underachieving students in the same variables and higher punctuations than normal-achieving students in most of the variables in which significant differences were detected. These results have clear educational implications.

  7. Differences in learning strategies, goal orientations, and self-concept between overachieving, normal-achieving, and underachieving secondary students.

    Directory of Open Access Journals (Sweden)

    JUAN LUIS CASTEJON

    2016-09-01

    Full Text Available The aims of this work were to identify and establish differential characteristics in learning strategies, goal orientations and self-concept between overachieving, normal-achieving and underachieving secondary students. A total of 1,400 Spanish first and second year high school students from the South-East geographical area participated in this study. Three groups of students were established: a group with underachieving students, a group with a normal level of achievement, and a third group with overachieving students. The students were assigned to each group depending on the residual punctuations obtained from a multiple regression analysis in which the punctuation of an IQ test was the predictor and a measure composed of the school grades of nine subjects was the criteria. The results of one-way ANOVA and the Games-Howell post-hoc test showed that underachieving students had significantly lower punctuations in all of the measures of learning strategies and learning goals, as well as all of the academic self-concept, personal self-concept, parental relationship, honesty, and personal stability factors. In contrast, overachieving students had higher punctuations than underachieving students in the same variables and higher punctuations than normal-achieving students in most of the variables in which significant differences were detected. These results have clear educational implications.

  8. Comparative Evaluation of Different Commercial Topical Natural Products Base Formulation on Wound Healing in Normal and Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Surayya Razali

    2016-10-01

    Full Text Available Summary. Wound healing is impaired and delayed in diabetes state. The aim of the study was to compare the effects of different topical natural products; Olive Heal, olive oil and vitamin E on the wound healing process in normal and diabetic rats. The study was carried out using 56 male Sprague-Dawley rats which were assigned randomly into 7 groups:  group I (normal wounded untreated, group II (normal wounded treated with Olive Heal, group III (diabetic wounded untreated, group IV (diabetic wounded treated with Jelonet®, group V (diabetic wounded treated with Olive Heal, group VI (diabetic wounded treated with olive oil, and group VII (diabetic wounded treated with palm vitamin E. Four full thickness excisions were made at the back of the rats. Wound healing was assessed by rate of wound closure and histological evaluation on the tenth day after wounding. All treatments were able to enhance the wound healing process by increasing rate of wound closure significantly (p0.05 on collagen deposition and neovascularization scores. However, all treatments showed high histological scores compared to group III. In conclusion, topical application of the natural products enhanced wound healing process in normal and diabetic rats. Industrial relevance. Wound healing impairment in diabetes mellitus patients is one of the complications which can debilitate patients. Natural products that can be used as topical dressing have been studied. This study was conducted to determine the ability of Olive Heal, olive oil and vitamin E in promoting wound healing. These treatments pose fewer side effects compared to the chemical drugs used commercially. In addition, antioxidant contents in these natural products may play a role to reduce radicals and act as scavengers against reactive species. It may protect the cells from oxidative damage thus expediting wound healing. This will explore the possibilities of commercializing these natural products as wound healing agents

  9. A study on the differences between oral squamous cell carcinomas and normal oral mucosas measured by Fourier transform infrared spectroscopy.

    Science.gov (United States)

    Fukuyama, Y; Yoshida, S; Yanagisawa, S; Shimizu, M

    1999-01-01

    We investigated the differences of Fourier transform infrared (FTIR) spectra between oral squamous cell carcinoma (OSCC) and normal gingival epithelium (NGE) or normal subgingival tissue (NST). We used 15 specimens of OSCC which had not been treated before measurement and 10 of NGE or NST. We also used cultured oral squamous cell carcinoma (COSCC) and the tissue (MSCC) which massed for 3 months after the cultured oral squamous cell carcinoma was transplanted into the lower back of a rat. Those tissue spectra were compared with the purified human collagens and human keratin. One half of every tissue specimen was measured with FTIR and the other half was investigated histologically. The differences of FTIR spectra between OSCC and NGE were observed in the bands between 1431 and 1482 cm(-1) and between 1183 and 1274 cm(-1). The shoulder at 1368 cm(-1) tended to disappear in OSCC, and the peaks at 1246 and 1083 cm(-1) found in NGE tended to shift to those at 1242 and 1086 cm(-1) in OSCC, respectively. The infrared spectrum of NST was noticed to be strongly influenced by the presence of collagen. Significant differences were also observed in the second derivative FTIR spectra between OSCC and NGE. Our data suggested that this infrared technique is applicable to clinical diagnostics.

  10. Renal tolerance to nonhomogenous irradiation: Comparison of observed effects to predictions of normal tissue complication probability from different biophysical models

    Energy Technology Data Exchange (ETDEWEB)

    Flentje, M.; Hensley, F.; Gademann, G.; Wannenmacher, M. (Univ. of Heidelberg (Germany)); Menke, M. (German Cancer Research Center, Heidelberg (Germany))

    1993-09-01

    A patient series was analyzed retrospectively as an example of whole organ kidney irradiation with an inhomogenous dose distribution to test the validity of biophysical models predicting normal tissue tolerance to radiotherapy. From 1969 to 1984, 142 patients with seminoma were irradiated to the paraaortic region using predominantly rotational techniques which led to variable but partly substantial exposure of the kidneys. Median follow up was 8.2 (2.1-21) years and actuarial 10-year survival (Kaplan-Meier estimate) 82.8%. For all patients 3-dimensional dose distributions were reconstructed and normal tissue complication probabilities for the kidneys were generated from the individual dose volume histograms. To this respect different published biophysical algorithms were introduced in a 3-dimensional-treatment planning system. In seven patients clinically manifest renal impairment was observed (interval 10-84 months). An excellent agreement between predicted and observed effects was seen for two volume-oriented models, whereas complications were overestimated by an algorithm based on critical element assumptions. Should these observations be confirmed and extended to different types of organs corresponding algorithms could easily be integrated into 3-dimensional-treatment planning programs and be used for comparing and judging different plans on a more biologically oriented basis.

  11. Enthalpy difference between conformations of normal alkanes: Raman spectroscopy study of n-pentane and n-butane.

    Science.gov (United States)

    Balabin, Roman M

    2009-02-12

    Conformation equilibrium in normal pentane (C(5)H(12)) was studied by the low-temperature gas-phase Raman spectroscopy method. A special retroreflecting multipass cell was constructed. Gas-phase (vapor) spectra were recorded in the temperature region of -130.3 to -23.1 degrees C and in the spectral range below 500 cm(-1). The peaks of trans-trans (399.0 cm(-1)), trans-gauche (328.9 cm(-1)), and gauche(+)-gauche(+) (267.1 cm(-1)) conformers (rotamers) of n-pentane were assigned using quantum chemistry data (MP2 and B3LYP methods with aug-cc-pVTZ basis set). The contour of each line was deconvoluted using ab initio data to obtain precise total integral intensity. The intensities at different temperatures were used to evaluate the enthalpy (energy) difference between trans-gauche and trans-trans (DeltaH(tg) = 618 +/- 6 cal/mol), and gauche(+)-gauche(+) and trans-trans (DeltaH(g+g+) = 940 +/- 20 cal/mol) conformers. Normal butane (n-butane) measurements under similar experimental conditions have been taken to understand the chain length influence. The C(4)H(10) enthalpy difference value has been evaluated (DeltaH(g) = 660 +/- 22 cal/mol). The results are compared with published experimental and theoretical data. The data presented here can be used as a reference for quantum chemistry calculations of conformation equilibrium in n-butane and n-pentane.

  12. Difference in gene expression of macrophage between normal spleen and portal hypertensive spleen idendified by cDNA microarray

    Institute of Scientific and Technical Information of China (English)

    Feng Yan; Xiao-Min Wang

    2007-01-01

    AIM: To identify the difference in gene expression of microphage (Mφ) between normal spleen and portal hypertensive spleen using cDNA microarrays and find new gene functions associated with hypersplenism in portal hypertension.METHODS: The Biostar-H140s chip containing 14112 spots of cDNAs were used to investigate the difference of the expression. The total RNA extracted from macrophages isolated from both normal spleen and portal hypertensive spleen was reversely transcribed to cDNA with the incorporation of fluorescent (cy3 and cy5) labeled dCTP to prepare the hybridization probes.After hybridization, the gene chip was scanned for the fluorescent intensity. The differentially expressed genes were screened. That was repeated three times,and only the genes which had differential expression in all three chips were considered to be associated with hypersplenism in portal hypertension.RESULTS: Eight hundred and ninety-six, 1330 and 898 genes were identified to be differentially expressed in three chips, respectively. One hundred and twenty-one genes (0.86%) were identified to be differentially expressed in all three chips, including 21 up-regulated genes and 73 down-regulated genes. The differentially expressed genes were related to ionic channel and transport protein, cyclin, cytoskeleton, cell receptor, cell signal conduct, metabolism, immune, and so on. These genes might be related to the hypersplenism in portal hypertension.CONCLUSION: The investigations based on cDNA microarray can screen differentially expressed genes of macrophages between normal spleen and portal hypertensive spleen, thus may provide a new idea in studying the pathogenesis of hypersplenism in portal hypertension.

  13. Production of satellite-derived aerosol climate data records: current status of the ESA Aerosol_cci project

    Science.gov (United States)

    de Leeuw, Gerrit; Holzer-Popp, Thomas; Pinnock, Simon

    2015-04-01

    and the Aerosol_cci team Within the ESA Climate Change Initiative (CCI) project Aerosol_cci (Phase 1: 2010 -2014; Phase 2: 2014-2017) intensive work has been conducted to improve algorithms for the retrieval of aerosol information from European sensors ATSR (3 algorithms), PARASOL, MERIS (3 algorithms), synergetic AATSR/SCIAMACHY, OMI and GOMOS. Whereas OMI and GOMOS were used to derive absorbing aerosol index and stratospheric extinction profiles, respectively, Aerosol Optical Depth (AOD) and Ångström coefficient were retrieved from the other sensors. The cooperation between the project partners, including both the retrieval teams and independent validation teams, has resulted in a strong improvement of most algorithms. In particular the AATSR retrieved AOD is qualitatively similar to that from MODIS, usually taken as the standard, MISR and SeaWiFS. This conclusion has been reached form several different ways of validation of the L2 and L3 products, using AERONET sun photometer data as the common ground-truth for the application of both 'traditional' statistical techniques and a 'scoring' technique using spatial and temporal correlations. Quantitatively, the limited AATSR swath width of 500km results in a smaller amount of data. Nevertheless, the assimilation of AATSR-retrieved AOD, together with MODIS data, contributes to improving the in the ECMWF climate model results. In addition to the multi-spectral AOD, and thus the Ångström Exponent, also a per-pixel uncertainty is provided and validated. By the end of Aerosol_cci Phase 1 the ATSR algorithms have been applied to both ATSR-2 and AATSR resulting in an AOD time series of 17 years. In phase 2 this work is continued with a focus on the further improvement of the ATSR algorithms as well as those for the other instruments and algorithms, mentioned above, which in phase 1 were considered less mature. The first efforts are on the further characterization of the uncertainties and on better understanding of the

  14. Modifications of glycosphingolipid profile and synthesis in normal rat fibroblasts and in syngeneic neoplastic cells at different subculture stages.

    Science.gov (United States)

    Colombo, I; Sottocornola, E; Moretti, S; Meloni, M A; Pippia, P; Berra, B

    2000-05-31

    Glycosphingolipids are plasma membrane macromolecules involved in diversified recognition functions on the cell surface resulting in modulation of cell adhesion and differentiation. As the in vitro cellular system of the neoplastic cell line SGS/4A and syngeneic normal fibroblasts (FG) represents a useful tool for studies on molecular mechanisms regulating cell adhesion, neoplastic transformation and cellular ageing, we studied the changes of glycosphingolipid and of the enzymes involved in their metabolism in both cultured cells at different subculture stages. The FG subculture progression induces a drastic decrease of total glycosphingolipid content with consistent alterations in the molecular composition. In particular, a significant decrease of GM(3), a slight increase of GD(1a), the disappearance of 'b'-series gangliosides and the drastic reduction of triosylceramides were observed. On the contrary, the increasing number of SGS/4A subcultures, characterized by a specific and different glycosphingolipid composition as compared with FG cells, does not cause modifications. Although glycosyltransferase activity levels quite well parallel the glycosphingolipid patterns and can account for the noted variations, the mRNA expression analysis of two glycosyltransferases suggests that the in vitro cell ageing of normal rat fibroblasts causes drastic changes in the glycosphingolipid profile through the regulation, at either the transcriptional or post-translational level, of some biosynthetic enzymes.

  15. Satellite-derived changes in the permafrost landscape of central Yakutia, 2000-2011: wetting, drying, and fires

    Science.gov (United States)

    Boike, Julia; Grau, Thomas; Heim, Birgit; Günther, Frank; Langer, Moritz; Muster, Sina; Gouttevin, Isabelle; Lange, Stephan

    2016-04-01

    The focus of this research has been on detecting changes in lake areas, vegetation, land surface temperatures, and the area covered by snow, using data from remote sensing. The study area covers the main (central) part of the Lena River catchment in the Yakutia region of Siberia (Russia), extending from east of Yakutsk to the central Siberian Plateau, and from the southern Lena River to north of the Vilyui River. Approximately 90% of the area is underlain by continuous permafrost. Remote sensing products were used to analyze changes in water bodies, land surface temperature (LST), and leaf area index (LAI), as well as the occurrence and extent of forest fires, and the area and duration of snow cover. The remote sensing analyses (for LST, snow cover, LAI, and fire) were based on MODIS-derived NASA products for 2000 to 2011. Changes in water bodies were calculated from two mosaics of (USGS) Landsat high resolution (30 m) satellite images from 2002 and 2009. Within the study area's 315,000 km² the total area covered by lakes increased by 17.5% between 2002 and 2009, but this increase varied in different parts of the study area, ranging between 11% and 42%. The land surface temperatures showed a consistent warming trend, with an average increase of about 0.12°C/year. The average rate of warming during the April-May transition period was 0.15°C/year and 0.19°C/year in the September-October period, but ranged up to 0.45°C/year in some areas during April-May. Regional differences in the rates of land surface temperature change, and possible reasons for the temperature changes, are discussed with respect to changes in the land cover. Our analysis of a broad spectrum of variables over the study area suggests that the spring warming trend is very likely to be due to changes in the area covered by snow. The warming trend observed in fall does not, however, appear to be directly related to any changes in the area of snow cover, or to the atmospheric conditions, or to the

  16. Towards Calibration of Sentinel 3 Data: Validation of Satellite-Derived SST Against In Situ Coastal Observations of the Portuguese Marine Waters

    Science.gov (United States)

    Vicente, Ricardo; Esteves, Rita; Lamas, Luisa; Pinto, Jose Paulo; Almeida, Sara; de Azevedo, Eduardo; Correia, Cecilia; Reis, Francisco

    2016-08-01

    Validation of future Sentinel-3 SLSTR data in the Eastern Atlantic Ocean was analysed here through a comparison of satellite-derived STT against in situ mooring buoys observations.SSTskin retrieved from IR satellite radiometers on- board ERS 1-2, Envisat, and Aqua, and concurrent SSTbulk measured with 14 buoy thermistors located at 1m depth were used to assess the statistical relationships between these datasets, with 20038 match- ups spanning from 1996 to 2015.As expected, results showed consistency between SSTskin and SSTbulk, exhibiting a correlation coefficient on the order of 98 %. Biases of both (A)ATSR and MODIS for day-time suggest a warmer satellite skin retrieval of + 0.15o and + 0.06o, respectively. For the night-time dataset, biases of - 0.25o and - 0.17o for (A)A TSR and MODIS, respectively, indicate cooler skin retrievals and reveal an inversion of the upper ocean thermic gradient. The RMSE ´s found were 0.53o for (A)ATSR and 0.41o for MODIS datasets.

  17. A photogrammetric DEM of Greenland based on 1978-1987 aerial photos: validation and integration with laser altimetry and satellite-derived DEMs

    Science.gov (United States)

    Korsgaard, N. J.; Kjaer, K. H.; Nuth, C.; Khan, S. A.

    2014-12-01

    Here we present a DEM of Greenland covering all ice-free terrain and the margins of the GrIS and local glaciers and ice caps. The DEM is based on the 3534 photos used in the aero-triangulation which were recorded by the Danish Geodata Agency (then the Geodetic Institute) in survey campaigns spanning the period 1978-1987. The GrIS is covered tens of kilometers into the interior due to the large footprints of the photos (30 x 30 km) and control provided by the aero-triangulation. Thus, the data are ideal for providing information for analysis of ice marginal elevation change and also control for satellite-derived DEMs.The results of the validation, error assessments and predicted uncertainties are presented. We test the DEM using Airborne Topographic Mapper (IceBridge ATM) as reference data; evaluate the a posteriori covariance matrix from the aero-triangulation; and co-register DEM blocks of 50 x 50 km to ICESat laser altimetry in order to evaluate the coherency.We complement the aero-photogrammetric DEM with modern laser altimetry and DEMs derived from stereoscopic satellite imagery (AST14DMO) to examine the mass variability of the Northeast Greenland Ice Stream (NEGIS). Our analysis suggests that dynamically-induced mass loss started around 2003 and continued throughout 2014.

  18. Decision making based on global flood forecasts and satellite-derived inundation maps in data-sparse regions

    Science.gov (United States)

    Revilla-Romero, Beatriz; Hirpa, Feyera A.; Thielen-del Pozo, Jutta; Salamon, Peter; Brakenridge, G. Robert; Pappenberger, Florian; De Groeve, Tom

    2016-04-01

    Early flood warning and real-time monitoring systems play a key role in flood risk reduction and disaster response decisions. Global-scale flood forecasting and satellite-based flood detection systems are currently operating, however their reliability for decision making applications needs to be assessed. In this study, we performed comparative evaluations of several operational global flood forecasting and flood detection systems, using major flood events recorded over 2012-2014. Specifically, we evaluated the spatial extent and temporal characteristics of flood detections from the Global Flood Detection System (GFDS) and the Global Flood Awareness System (GloFAS). Furthermore, we compared the GFDS flood maps with those from NASA's two Moderate Resolution Imaging Spectroradiometer (MODIS) sensors. Results reveal that: 1) general agreement was found between the GFDS and MODIS flood detection systems, 2) large differences exist in the spatio-temporal characteristics of the GFDS detections and GloFAS forecasts, and 3) the quantitative validation of global flood disasters in data-sparse regions is highly challenging. Overall, the satellite remote sensing provides useful near real-time flood information that can be useful for risk management. We highlight the known limitations of global flood detection and forecasting systems, and propose ways forward to improve the reliability of large scale flood monitoring tools.

  19. Influence of Desert Dust Intrusions on Ground-based and Satellite Derived Ultraviolet Irradiance in Southeastern Spain

    Science.gov (United States)

    Krotkov, Nickolay A.; Anton, Manuel; Valenzuela, Antonio; Roman, Roberto; Lyamani, Hassan; Arola, Antti; Olmo, Francisco J.; Alados-Arboledas

    2012-01-01

    The desert dust aerosols strongly affect propagation of solar radiation through the atmosphere, reducing surface irradiance available for photochemistry and photosynthesis. This paper evaluates effects of desert dust on surface UV erythemal irradiance (UVER), as measured by a ground-based broadband UV radiometer and retrieved from the satellite Ozone Monitoring Instrument (OMI) at Granada (southern Spain) from January 2006 to December 2010. The dust effects are characterized by the transmittance ra tio of the measured UVER to the corresponding modeled clear sky value. The transmittance has an exponential dependency on aerosol optical depth (AOD), with minimum values of approximately 0.6 (attenuation of approximately 40%). The OMI UVER algorithm does not account for UV aerosol absorption, which results in overestimation of the ground-based UVER especially during dust episodes with a mean relative difference up to 40%. The application of aerosol absorption post-correction method reduces OMI bias up to approximately 13%. The results highlight great effect of desert dust on the surface UV irradiance in regions like southern Spain, where dust intrusions from Sahara region are very frequent.

  20. Improvements of Satellite Derived Cyclonic Rainfall Over The North Atlantic and Implications Upon The Air-sea Interaction

    Science.gov (United States)

    Klepp, C.; Bakan, S.; Grassl, H.

    Case studies of rainfall, derived from Special Sensor Microwave/Imager (SSM/I) satellite data, during the passage of individual cyclones over the North Atlantic are presented to enhance the knowledge of rainfall processes asso ciated with frontal sys- tems. The Multi-Satellite-Technique (MST) is described to receive a complete cov- erage of the North Atlantic twice a day. Different SSM/I precipitation algorithms (Bauer and Schlüssel, Ferraro, Wentz) have been tested for individual cyclones and were compared to the Global Precipitation Climatology Project (GPCP) data sets and NWP model results (ECMWF, REMO). An independent rainfall pattern and -intensity vali dation method is presented using voluntary observing ship (VOS) data sets and AVHRR images. Intense storms occur very often in the wintertime period with cold fronts propagating far south over the North Atlantic. Large frontal conditions are mostly well represented in all tested data sets. Following upstream numerous showers are usually embedded in the cellular structures of the cold air on the backside of post frontal subsidences. Cold air clusters frequently occur in these backside regions which produce heavy convective rainfall events. Espe cially in the region off Newfoundland at 50o North, where very cold air within arctic cold air outbreaks is advected over the warm waters of the Gulfstream current, these heavily raining clusters often rapidly form into mesoscale storms. These small but intense cyclones up to 1500km in diame- ter are characterized by very heavy precipitation over the entire region and may occur every three days in wintertime. The storms can be easily identified on AVHRR im- ages. Only the SSM/I rainfall algorithm of Bauer and Schlüssel in combination with the presented MST is sensitive enough to detect the correct patterns and intensities of rainfall for those cyclone types over the North Atlantic, whereas the GPCP products fail in recognizing any rainfall at all. A SLP study figures

  1. Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

    Science.gov (United States)

    Zhu, Jianfeng; Zhang, Lifeng; Li, Wanfeng; Han, Suying; Yang, Wenhua; Qi, Liwang

    2013-01-01

    Quantitative real-time reverse transcription polymerase chain reaction (qPCR), a sensitive technique for gene expression analysis, depends on the stability of the reference genes used for data normalization. Caragana intermedia, a native desert shrub with strong drought-resistance, sand-fixing capacity and high forage value that is widespread in the desert land of west and northwest China, has not been investigated regarding the identification of reference genes suitable for the normalization of qPCR data. In this study, 10 candidate reference genes were analyzed in C. intermedia subjected to different abiotic (osmotic, salt, cold and heat) stresses, in two distinct plant organs (roots and leaves). The expression stability of these genes was assessed using geNorm, NormFinder and BestKeeper algorithms. The best-ranked reference genes differed across the different sets of samples, but UNK2, PP2A and SAND were the most stable across all tested samples. UNK2 and SAND would be appropriate for normalizing gene expression data for salt-treated roots, whereas the combination of UNK2, SAND and EF-1α would be appropriate for salt-treated leaves. UNK1, UNK2 and PP2A would be appropriate for PEG-treated (osmotic) roots, whereas the combination of TIP41 and PP2A was the most suitable for PEG-treated leaves. SAND, PP2A and TIP41 exhibited the most stable expression in heat-treated leaves. In cold-treated leaves, SAND and EF-1α were the most stably expressed. To further validate the suitability of the reference genes identified in this study, the expression levels of DREB1 and DREB2 (homologs of AtDREB1 and AtDREB2) were studied in parallel. This study is the first systematic analysis for the selection of superior reference genes for qPCR in C. intermedia under different abiotic stress conditions, and will benefit future studies on gene expression in C. intermedia and other species of the leguminous genus Caragana.

  2. Reference gene selection for quantitative real-time PCR normalization in Caragana intermedia under different abiotic stress conditions.

    Directory of Open Access Journals (Sweden)

    Jianfeng Zhu

    Full Text Available Quantitative real-time reverse transcription polymerase chain reaction (qPCR, a sensitive technique for gene expression analysis, depends on the stability of the reference genes used for data normalization. Caragana intermedia, a native desert shrub with strong drought-resistance, sand-fixing capacity and high forage value that is widespread in the desert land of west and northwest China, has not been investigated regarding the identification of reference genes suitable for the normalization of qPCR data. In this study, 10 candidate reference genes were analyzed in C. intermedia subjected to different abiotic (osmotic, salt, cold and heat stresses, in two distinct plant organs (roots and leaves. The expression stability of these genes was assessed using geNorm, NormFinder and BestKeeper algorithms. The best-ranked reference genes differed across the different sets of samples, but UNK2, PP2A and SAND were the most stable across all tested samples. UNK2 and SAND would be appropriate for normalizing gene expression data for salt-treated roots, whereas the combination of UNK2, SAND and EF-1α would be appropriate for salt-treated leaves. UNK1, UNK2 and PP2A would be appropriate for PEG-treated (osmotic roots, whereas the combination of TIP41 and PP2A was the most suitable for PEG-treated leaves. SAND, PP2A and TIP41 exhibited the most stable expression in heat-treated leaves. In cold-treated leaves, SAND and EF-1α were the most stably expressed. To further validate the suitability of the reference genes identified in this study, the expression levels of DREB1 and DREB2 (homologs of AtDREB1 and AtDREB2 were studied in parallel. This study is the first systematic analysis for the selection of superior reference genes for qPCR in C. intermedia under different abiotic stress conditions, and will benefit future studies on gene expression in C. intermedia and other species of the leguminous genus Caragana.

  3. Assessment of strain and dyssynchrony in normal fetuses using speckle tracking echocardiography - comparison of three different ultrasound probes.

    Science.gov (United States)

    Rolf, Daniel; Schmidt, Rene; Möllers, Mareike; Oelmeier de Murcia, Kathrin; Braun, Janina; Hammer, Kerstin; Klockenbusch, Walter; Schmitz, Ralf

    2017-07-28

    To evaluate segmental left (LV-S) and right (RV-S) ventricular strain as well as longitudinal mechanical myocardial dyssynchrony as a time difference between peaks in strain of both ventricles in fetuses (two-chamber-dyssynchrony, 2C-DYS) using speckle tracking echocardiography (STE). The aim of our study was to evaluate the influence of data acquisition on the results of STE measurement using different ultrasound probes. We prospectively recorded cardiac cycles of four-chamber views of 56 normal fetuses with three different ultrasound probes and analyzed them offline with speckle tracking imaging software. Furthermore, we looked at a possible influence of heartbeat variability (beat-to-beat variability). The evaluation of the parameters was feasible with all three probes in 53 cases. There was no influence of heartbeat variability and no noticeable differences in 2C-DYS, LV-S and RV-S in all cases and for all three probes determined. Assessment of strain and dyssynchrony using STE with three different probes is comparable. Further research is needed to validate dyssynchrony as a predictor for fetal outcome.

  4. Role of VEGF receptors in normal and psoriatic human keratinocytes: evidence from irradiation with different UV sources.

    Directory of Open Access Journals (Sweden)

    Jian-Wei Zhu

    Full Text Available Vascular endothelial growth factor (VEGF promotes angiogenesis and plays important roles both in physiological and pathological conditions. VEGF receptors (VEGFRs are high-affinity receptors for VEGF and are originally considered specific to endothelial cells. We previously reported that VEGFRs were also constitutively expressed in normal human keratinocytes and overexpressed in psoriatic epidermis. In addition, UVB can activate VEGFRs in normal keratinocytes, and the activated VEGFR-2 signaling is involved in the pro-survival mechanism. Here, we show that VEGFRs were also upregulated and activated by UVA in normal human keratinocytes via PKC, and interestingly, both the activated VEGFR-1 and VEGFR-2 protected against UVA-induced cell death. As VEGFRs were over-expressed in psoriatic epidermis, we further investigated whether narrowband UVB (NB-UVB phototherapy or topical halomethasone monohydrate 0.05% cream could affect their expression. Surprisingly, the over-expressed VEGFRs in psoriatic epidermis were significantly attenuated by both treatments. During NB-UVB therapy, VEGFRs declined first in the basal, and then gradually in the upper psoriatic epidermis. VEGFRs were activated in psoriatic epidermis, their activation was enhanced by NB-UVB, but turned undetectable after whole therapy. This process was quite different from that by halomethasone, in which VEGFRs and phospho-VEGFRs decreased in a gradual, homogeneous manner. Our findings further suggest that UV-induced activation of VEGFRs serves as a pro-survival signal for keratinocytes. In addition, VEGFRs may be involved in the pathological process of psoriasis, and UV phototherapy is effective for psoriasis by directly modulating the expression of VEGFRs.

  5. Near Point of Convergence Break for Different Age Groups in Turkish Population with Normal Binocular Vision: Normative Data

    Directory of Open Access Journals (Sweden)

    Nihat Sayın

    2013-12-01

    Full Text Available Purpose: The purpose of this study was to evaluate the near point of convergence break in Turkish population with normal binocular vision and to obtain the normative data for the near point of convergence break in different age groups. Such database has not been previously reported. Material and Method: In this prospective study, 329 subjects with normal binocular vision (age range, 3-72 years were evaluated. The near point of convergence break was measured 4 times repeatedly with an accommodative target. Mean values of near point of convergence break were provided for these age groups (≤10, 11-20, 21-30, 31-40, 41-50, 51-60, and >60 years old. A statistical comparison (one-way ANOVA and post-hoc test of these values between age groups was performed. A correlation between the near point of convergence break and age was evaluated by Pearson’s correlation test. Results: The mean value for near point of convergence break was 2.46±1.88 (0.5-14 cm. Specifically, 95% of measurements in all subjects were 60 year-old age groups in the near point of convergence break values (p=0.0001, p=0.0001, p=0.006, p=0.001, p= 0.004. A mild positive correlation was observed between the increase in near point of convergence break and increase of age (r=0.355 (p<0.001. Discussion: The values derived from a relatively large study population to establish a normative database for the near point of convergence break in the Turkish population with normal binocular vision are in relevance with age. This database has not been previously reported. (Turk J Ophthalmol 2013; 43: 402-6

  6. ANALISA KESEHATAN TANAMAN PADI BERDASARKAN NILAI NORMALIZED DIFFERENCE VEGETATION INDEX (NDVI MENGGUNAKAN CITRA ASTER (STUDI KASUS : KABUPATEN INDRAMAYU - JAWA BARAT

    Directory of Open Access Journals (Sweden)

    Prasetyo Rahaldi

    2015-02-01

    Full Text Available Padi merupakan salah satu tanaman budidaya yang terpenting karena merupakan makanan pokok bagi 90% penduduk Indonesia. Oleh sebab itu dibutuhkan analisa yang cepat dan akurat mengenai kesehatan tanaman padi. Dalam Penelitian ini NDVI atau Normalized Difference Vegetation Index merupakan metode yang digunakan dalam membandingkan tingkat kehijauan vegetasi yang berasal dari citra ASTER. Dari nilai NDVI tersebut dapat diketahui klasifikasi kesehatan tanaman padi. Dalam penelitian ini klasifikasi kesehatan tanaman padi dibagi menjadi  4 kelas. Kesehatan sangat baik terdapat pada rentang nilai NDVI 0.721-0.92, untuk kesehatan baik rentang nilai NDVI antara 0.421-0.72, dan nilai NDVI kesehatan normal terdapat pada rentang 0.221-0.42, sedangkan kesehatan buruk nilai NDVI 0.11-0.22. Selain itu juga menggunakan data Field Spectrometer sebanyak 14 titik sebagai data lapangan yang digunakan untuk proses validasi. Validasi ini mempunyai koefisien korelasi (R sebesar 0.829. Sehingga dapat dikatakan antara nilai hasil prediksi dan hasil pengukuran lapangan berkolerasi sebesar 82,9 %. Dengan data citra ASTER juga dihasilkan pustaka spektral dan peta kesehatan tanaman padi, dalam pustaka spektral semakin sehat tanaman nilai Digital Number pada band 2 semakin kecil. Sedangkan band 3 banyak dipantulkan atau tidak digunakan sehingga nilai Digital Number pada tanaman padi yang semakin sehat, nilainya semakin tinggi. Sedangkan dalam peta kesehatan tanaman padi klasifikasi kesehatan buruk luas areanya 3.949.560 Ha. Pada klasifikasi kesehatan normal luas areanya 14.877.315 Ha. Sedangkan pada klasifikasi kesehatan baik luas areanya 9.846.833 Ha dan pada klasifikasi kesehatan sangat baik luas areanya 8.922.892.

  7. Differences in the haematological profile of healthy 70 year old men and women: normal ranges with confirmatory factor analysis

    Directory of Open Access Journals (Sweden)

    Taylor Michelle

    2010-06-01

    Full Text Available Abstract Background Reference ranges are available for different blood cell counts. These ranges treat each cell type independently and do not consider possible correlations between cell types. Methods Participants were identified from the Community Health Index as survivors of the 1947 Scottish Mental Survey, all born in 1936, who were resident in Lothian (potential n = 3,810 and invited to participate in the study. Those who consented were invited to attend a Clinical Research Facility where, amongst other assessments, blood was taken for full blood count. First we described cell count data and bivariate correlations. Next we performed principal components analysis to identify common factors. Finally we performed confirmatory factor analysis to evaluate suitable models explaining relationships between cell counts in men and women. Results We examined blood cell counts in 1027 community-resident people with mean age 69.5 (range 67.6-71.3 years. We determined normal ranges for each cell type using Q-Q plots which showed that these ranges were significantly different between men and women for all cell types except basophils. We identified three principal components explaining around 60% of total variance of cell counts. Varimax rotation indicated that these could be considered as erythropoietic, leukopoietic and thrombopoietic factors. We showed that these factors were distinct for men and women by confirmatory factor analysis: in men neutrophil count was part of a 'thrombopoietic' trait whereas for women it was part of a 'leukopoietic' trait. Conclusions First, normal ranges for haematological indices should be sex-specific; at present this only pertains to those associated with erythrocytes. Second, differences between individuals across a range of blood cell counts can be explained to a considerable extent by three major components, but these components are not the same in men and women.

  8. Food choice, eating behavior, and food liking differs between lean/normal and overweight/obese, low-income women.

    Science.gov (United States)

    Dressler, Heidi; Smith, Chery

    2013-06-01

    The higher rate of obesity among low-income women has widely been attributed to environmental barriers; however, many low-income women are still able to maintain a healthy weight despite obesogenic environments. To better understand personal and behavioral attributes related to food choice and weight, overweight/obese women and lean/normal weight women living in similar low-income environments, participated in focus groups, and taste testing sessions to investigate food liking (n=83). During focus groups, lean/normal weight participants reported that health was influential in food choice, while overweight/obese participants expressed cost as being more of a factor. Both BMI (kg/m(2)) groups reported that taste was of greatest importance. Personal factors, like emotional eating, and overeating were also discussed with differences noted between BMI (kg/m(2)) groups. Quantitative data also showed cost to be more important for overweight/obese women. Taste testing results revealed that overweight/obese participants had a higher overall liking for both healthy and less healthy foods, as well as other food categories. Additionally, these women had a higher liking of fat in the context of spreadable fats. Our results show that a variety of complex factors interact to influence eating behavior and present weight status of women living in similarly impoverished environments. However, findings from this exploratory study should be confirmed through further research. Copyright © 2013 Elsevier Ltd. All rights reserved.

  9. Effect of different amounts of coffee on dietary intake and appetite of normal-weight and overweight/obese individuals.

    Science.gov (United States)

    Gavrieli, A; Karfopoulou, E; Kardatou, E; Spyreli, E; Fragopoulou, E; Mantzoros, C S; Yannakoulia, M

    2013-06-01

    To investigate the effects of different coffee amounts on dietary intake and appetite feelings in normal-weight and overweight/obese individuals. Thirty-three volunteers (16 normal-weight, 17 overweight/obese) participated in three trials: they consumed a standard breakfast along with 200 ml of either coffee with 3 or 6 mg caffeine/kg body weight (Coffee 3 and Coffee 6, respectively), or water. At fasting and at standard time points for the 3 h following breakfast/drink consumption participants recorded their appetite feelings on visual analogue scales. At 180 min, participants consumed an ad libitum meal and the next day they recalled their food intake during the experimental day. A significant intervention effect was found for the energy intake of the ad libitum meal (P = 0.05) and of the whole day (P = 0.02) only in overweight/obese individuals. Specifically, Coffee 6 resulted in a reduced energy intake during the ad libitum meal compared to Coffee 3 (P = 0.03) and in the total day compared to both water (P = 0.04) and Coffee 3 (P = 0.008). No effect was observed for the appetite feelings. A moderate coffee amount can effectively reduce energy intake in the following meal and in the total day compared to lower or no coffee intake in overweight/obese participants. Copyright © 2013 The Obesity Society.

  10. Cytological Differences in the Localization of Glucocorticoid Receptor-Like Imnunoreactivity in the Normal and Transplanted Pituitary Pars Intermedia

    Science.gov (United States)

    Iturriza, Fermlín C.; Dumm, César L. A. Gómez

    1992-01-01

    Glucocorticoid receptor-like immunoreactivity (GCRI) was found in the normal pituitary pars intermedia (PI) when immunohistochemistry was used. Since in previous studies we described two kinds of cells in the denervated (grafted) PI, i.e., “light cells” (overactive cells which do not contain detectable melanocyte stimulating hormone) and “dark cells” (hypoactive cells which contain the hormone), it was decided to investigate whether different patterns of distribution of the receptors could be detected in the grafted gland when compared with the intact PI. Intact glands showed the receptors located in the nucleus. In transplanted glands, it was observed that light cells showed receptors in both the nuclei and the cytoplasm; on the other hand, dark cells displayed them in the nuclei only, as is the case in all cells of the normal PI. We had previously interpreted dark cells as dopamine-indifferent, whereas light cells were considered dopamine-sensitive. The changes in the distribution of GCR after denervation by grafting, which only affected the light cells, support the view of other authors that GCR of. the pars intermedia are under the influence of dopamine and reinforce our opinion that dark cells are dopamine-indifferent PMID:1571397

  11. Pattern of normal age-related regional differences in white matter microstructure is modified by vascular risk.

    Science.gov (United States)

    Kennedy, Kristen M; Raz, Naftali

    2009-11-10

    Even successful aging is associated with regional brain shrinkage and deterioration of the cerebral white matter. Aging also brings about an increase in vascular risk, and vascular impairment may be a potential mechanism behind the observed patterns of aging. The goals of this study were to characterize the normal age differences in white matter integrity in several brain regions across the adult life span and to assess the modifying effect of vascular risk on the observed pattern of regional white matter integrity. We estimated fractional anisotropy and diffusivity of white matter in nine cerebral regions of interest in 77 healthy adults (19-84 years old). There was a widespread reduction of white matter anisotropy with age, and prefrontal and occipital regions evidenced the greatest age-related differences. Diffusivity increased with age, and the magnitude of age differences increased beginning with the middle of the fifth decade. Vascular risk factors modified age differences in white matter integrity. Clinically diagnosed and treated arterial hypertension was associated with reduced white matter anisotropy and increased diffusivity beyond the effects of age. In the normotensive participants, elevation of arterial pulse pressure (a surrogate of arterial stiffness) was linked to deterioration of the white matter integrity in the frontal regions. Although the causal role of vascular risk in brain aging is unclear, the observed pattern of effects suggests that vascular risk may drive the expansion of age-related white matter damage from anterior to posterior regions.

  12. Photoelastic stress analysis of endodontically treated teeth restored with different post systems: normal and alveolar bone resorption cases.

    Science.gov (United States)

    Ma, Jinbao; Miura, Hiroyuki; Okada, Daizo; Yusa, Koichiro

    2011-01-01

    The present study examined the influence of different post materials and their lengths on the mechanical stress of endodontically treated incisor roots in two alveolar bone conditions. Two-dimensional photoelastic models were fabricated to simulate the endodontically treated maxillary central incisors restored with three kinds of posts materials (low Young's modulus glass fiber post, high Young's modulus glass fiber post, and prefabricated stainless steel post) and two post lengths (8 and 4 mm). Completed models were placed in a transmission polariscope and loaded with a static force of 150 N at 45° to the tooth axis. Photoelastic photographs and the magnitudes of fringe order revealed stress distribution in the root, and suggest that the glass fiber post with a low Young's modulus and long length can reduce the stress concentration both in normal and alveolar bone resorption conditions.

  13. Response of remotely sensed Normalized Difference Water Deviation Index to the 2006 Drought of eastern Sichuan Basin

    Institute of Scientific and Technical Information of China (English)

    ZHANG WenJiang; LU QiFeng; GAO ZhiQiang; PENG Jian

    2008-01-01

    One of the most serious droughts in last century occurred in eastern Sichuan Basin in the summer of 2006 (hereinafter called the Drought). The response of Moderate Resolution Imaging Spectroradiometer (MODIS, boarding on NASA satellites of Terra and Aqua) to the Drought was analyzed in order to reach one practicable monitoring solution for regional soil moisture. Temporal process and spatial extension of the Drought were firstly estimated with ground meteorological and hydrological observations. Then, for the whole region of Sichuan and Chongqing, the remotely sensed Normalized Difference Water In-dex (NDWI) for the summers of 2001-2006 were calculated based on 8-day composite MODIS products, which were further used to construct a new water index (Normalized Difference Water Deviation Index, NDWDI) to examine the sensitivity of remote sensing in the Drought. The study showed that the NDWDI is more sensitive to regional drought than other absolute-soil-moisture-based indices. With the new index, the study extracted the spatial-temporal characteristics of the 2006 Drought, and explored its developing and withdrawing processes, which agreed with related statistics. Compared with ground method of drought observation, the NDWDI-based remote sensing solution of this paper is more pref-erable and practicable in that the local soil properties of water consumption and supply are implicitly taken into account, and the spatial representativity limit of ground observation is circumvented to a degree as satellite remotely senses the earth surface in a way of two-dimensional pixel matrix. So, the NDWDI-based method can be used to monitor regional soil water stress situation more practically and efficiently.

  14. Response of remotely sensed Normalized Difference Water Deviation Index to the 2006 Drought of eastern Sichuan Basin

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    One of the most serious droughts in last century occurred in eastern Sichuan Basin in the summer of 2006 (hereinafter called the Drought). The response of Moderate Resolution Imaging Spectroradiometer (MODIS, boarding on NASA satellites of Terra and Aqua) to the Drought was analyzed in order to reach one practicable monitoring solution for regional soil moisture. Temporal process and spatial extension of the Drought were firstly estimated with ground meteorological and hydrological observations. Then, for the whole region of Sichuan and Chongqing, the remotely sensed Normalized Difference Water In- dex (NDWI) for the summers of 2001―2006 were calculated based on 8-day composite MODIS products, which were further used to construct a new water index (Normalized Difference Water Deviation Index, NDWDI) to examine the sensitivity of remote sensing in the Drought. The study showed that the NDWDI is more sensitive to regional drought than other absolute-soil-moisture-based indices. With the new index, the study extracted the spatial-temporal characteristics of the 2006 Drought, and explored its developing and withdrawing processes, which agreed with related statistics. Compared with ground method of drought observation, the NDWDI-based remote sensing solution of this paper is more pref- erable and practicable in that the local soil properties of water consumption and supply are implicitly taken into account, and the spatial representativity limit of ground observation is circumvented to a degree as satellite remotely senses the earth surface in a way of two-dimensional pixel matrix. So, the NDWDI-based method can be used to monitor regional soil water stress situation more practically and efficiently.

  15. Estradiol differently affects melanin synthesis of malignant and normal melanocytes: a relationship with clock and clock-controlled genes.

    Science.gov (United States)

    Poletini, Maristela Oliveira; de Assis, Leonardo Vinicius Monteiro; Moraes, Maria Nathalia; Castrucci, Ana Maria de Lauro

    2016-10-01

    Melanin production within melanocytes is regulated, among others, by estradiol, whose effects on melanogenesis are still not completely elucidated. Here we show that although 10(-7) M 17β-estradiol (E2) increased tyrosinase mRNA levels in B16-F10 malignant melanocytes, there was a transient decrease and abolishment of the temporal variation of melanin content. Both parameters were much higher in the malignant than in normal Melan-a cells. Considering that silencing clock machinery in human melanocytes increases melanogenesis, we investigated clock gene expression in those cell lines. Except for Melan-a Bmal1 and B16-F10 Per2 expression of control cells, Per1, Per2, and Bmal1 expression increased independently of cell type or E2 treatment after 24 h. However, melanoma cells showed a marked increase in Per1 and Bma11 expression in response to E2 at the same time points, what may rule out E2 as a synchronizer agent since the expression of those genes were not in antiphase. Next, we investigated the expression of Xpa, a clock-controlled gene, which in Melan-a cells, peaked at 18 h, and E2 treatment shifted this peak to 24 h, whereas B16-F10 Xpa expression peaked at 24 h in both control and E2 group, and it was higher compared to Melan-a cells in both groups. Therefore, malignant and normal melanocytes display profound differences on core elements of the local clock, and how they respond to E2, what is most probably determinant of the differences seen on melanin synthesis and Tyrosinase and Xpa expression. Understanding these processes at the molecular level could bring new strategies to treat melanoma.

  16. Evaluating the difference between the normalized difference vegetation index and net primary productivity as the indicators of vegetation vigor assessment at landscape scale.

    Science.gov (United States)

    Xu, Chi; Li, Yutong; Hu, Jian; Yang, Xuejiao; Sheng, Sheng; Liu, Maosong

    2012-03-01

    Both the net primary productivity (NPP) and the normalized difference vegetation index (NDVI) are commonly used as indicators to characterize vegetation vigor, and NDVI has been used as a surrogate estimator of NPP in some cases. To evaluate the reliability of such surrogation, here we examined the quantitative difference between NPP and NDVI in their outcomes of vegetation vigor assessment at a landscape scale. Using Landsat ETM+ data and a process model, the Boreal Ecosystem Productivity Simulator, NPP distribution was mapped at a resolution of 90 m, and total NDVI during the growing season was calculated in Heihe River Basin, Northwest China in 2002. The results from a comparison between the NPP and NDVI classification maps show that there existed a substantial difference in terms of both area and spatial distribution between the assessment outcomes of these two indicators, despite that they are strongly correlated. The degree of difference can be influenced by assessment schemes, as well as the type of vegetation and ecozone. Overall, NDVI is not a good surrogate of NPP as the indicators of vegetation vigor assessment in the study area. Nonetheless, NDVI could serve as a fairish surrogate indicator under the condition that the target region has low vegetation cover and the assessment has relatively coarse classification schemes (i.e., the class number is small). It is suggested that the use of NPP and NDVI should be carefully selected in landscape assessment. Their differences need to be further evaluated across geographic areas and biomes.

  17. Correlation analysis of Normalized Different Vegetation Index (NDVI)difference series and climate variables in the Xilingole steppe,China from 1983 to 1999

    Institute of Scientific and Technical Information of China (English)

    GU Zhihui; CHEN Jin; SHI Peijun; XU Ming

    2007-01-01

    There is a crucial need in the study of global change to understand how terrestrial ecosystems respond to the climate system.It has been demonstrated by many researches that Normalized Different Vegetation Index (NDVI)time series from remotely sensed data,which provide effective information of vegetation conditions on a large scale with highly temporal resolution,have a good relation with meteorological factors.However,few of these studies have taken the cumulative property of NDVI time series into account.In this study,NDVI difference series were proposed to replace the original NDVI time series with NDVI difference series to reappraise the relationship between NDVI and meteorological factors.As a proxy of the vegetation growing process,NDVI difference represents net primary productivity of vegetation at a certain time interval under an environment controlled by certain climatic conditions and other factors.This data replacement is helpful to eliminate the cumulative effect that exist in original NDVI time series,and thus is more appropriate to understand how climate system affects vegetation growth in a short time scale.By using the correlation analysis method,we studied the relationship between NOAA/AVHRR ten-day NDVI difference series and corresponding meteorological data from 1983 to 1999 from 11 meteorological stations located in the Xilingole steppe in Inner Mongolia.The results show that:(1)meteorological factors are found to be more significantly correlation with NDVI difference at the biomass-rising phase than that at the falling phase;(2)the relationship between NDVI difference and climate variables varies with vegetation types and vegetation communities.In a typical steppe dominated by Leymus chinensis,temperature has higher correlation with NDVI difference than precipitation does,and in a typical steppe dominated by Stipa krylovii,the correlation between temperature and NDVI difference is lower than that between precipitation and NDVI difference.In a

  18. Different percentages of false-positive results obtained using five methods for the calculation of reference change values based on simulated normal and ln-normal distributions of data.

    Science.gov (United States)

    Lund, Flemming; Petersen, Per Hyltoft; Fraser, Callum G; Sölétormos, György

    2016-11-01

    Background Reference change values provide objective tools to assess the significance of a change in two consecutive results for a biomarker from an individual. The reference change value calculation is based on the assumption that within-subject biological variation has random fluctuation around a homeostatic set point that follows a normal (Gaussian) distribution. This set point (or baseline in steady-state) should be estimated from a set of previous samples, but, in practice, decisions based on reference change value are often based on only two consecutive results. The original reference change value was based on standard deviations according to the assumption of normality, but was soon changed to coefficients of variation (CV) in the formula (reference change value = ± Z ċ 2(½) ċ CV). Z is being dependent on the desired probability of significance, which also defines the percentages of false-positive results. The aim of this study was to investigate false-positive results using five different published methods for calculation of reference change value. Methods The five reference change value methods were examined using normally and ln-normally distributed simulated data. Results One method performed best in approaching the theoretical false-positive percentages on normally distributed data and another method performed best on ln-normally distributed data. The commonly used reference change value method based on two results (without use of estimated set point) performed worst both on normally distributed and ln-normally distributed data. Conclusions The optimal choice of method to calculate reference change value limits requires knowledge of the distribution of data (normal or ln-normal) and, if possible, knowledge of the homeostatic set point.

  19. Is magnetic resonance imaging of hepatic hemangioma any different in liver fibrosis and cirrhosis compared to normal liver?

    Energy Technology Data Exchange (ETDEWEB)

    Duran, Rafael, E-mail: rafael.duran@chuv.ch [Centre Hospitalier Universitaire Vaudois, University of Lausanne, Diagnostic and Interventional Radiology, Lausanne (Switzerland); Assistance-Publique Hôpitaux de Paris, APHP, Hôpital Beaujon, Department of Radiology, Clichy (France); Ronot, Maxime, E-mail: Maxime.ronot@bjn.aphp.fr [Assistance-Publique Hôpitaux de Paris, APHP, Hôpital Beaujon, Department of Radiology, Clichy (France); University Paris Diderot, Sorbonne Paris Cité, INSERM U773, Centre de Recherche Biomédicale Bichat-Beaujon, CRB3 Paris (France); Di Renzo, Sara, E-mail: Direnzo.sara@gmail.com [Assistance-Publique Hôpitaux de Paris, APHP, Hôpital Beaujon, Department of Radiology, Clichy (France); Gregoli, Bettina, E-mail: Bettinagregoli@yahoo.it [Assistance-Publique Hôpitaux de Paris, APHP, Hôpital Beaujon, Department of Radiology, Clichy (France); Van Beers, Bernard E., E-mail: Bernard.van-beers@bjn.aphp.fr [Assistance-Publique Hôpitaux de Paris, APHP, Hôpital Beaujon, Department of Radiology, Clichy (France); Vilgrain, Valérie, E-mail: Valerie.vilgrain@bjn.aphp.fr [Assistance-Publique Hôpitaux de Paris, APHP, Hôpital Beaujon, Department of Radiology, Clichy (France); University Paris Diderot, Sorbonne Paris Cité, INSERM U773, Centre de Recherche Biomédicale Bichat-Beaujon, CRB3 Paris (France)

    2015-05-15

    Highlights: • Hemangiomas were similar in patients with or without chronic liver disease on MRI. • Decrease in size & number of hemangiomas could start before the onset of cirrhosis. • T2 shine-through effect was less frequently observed in cirrhosis. - Abstract: Purpose: To compare qualitative and quantitative magnetic resonance (MR) imaging characteristics of hepatic hemangiomas in patients with normal, fibrotic and cirrhotic livers. Materials and methods: Retrospective, institutional review board approved study (waiver of informed consent). Eighty-nine consecutive patients with 231 hepatic hemangiomas who underwent liver MR imaging for lesion characterization were included. Lesions were classified into three groups according to the patients’ liver condition: no underlying liver disease (group 1), fibrosis (group 2) and cirrhosis (group 3). Qualitative and quantitative characteristics (number, size, signal intensities on T1-, T2-, and DW MR images, T2 shine-through effect, enhancement patterns (classical, rapidly filling, delayed filling), and ADC values) were compared. Results: There were 160 (69%), 45 (20%), and 26 (11%) hemangiomas in groups 1, 2 and 3, respectively. Lesions were larger in patients with normal liver (group 1 vs. groups 2 and 3; P = .009). No difference was found between the groups on T2-weighted images (fat-suppressed fast spin-echo (P = .82) and single-shot (P = .25)) and in enhancement patterns (P = .56). Mean ADC values of hemangiomas were similar between groups 1, 2 and 3 (2.11 ± .52 × 10{sup −3} mm{sup 2}/s, 2.1 ± .53 × 10{sup −3} mm{sup 2}/s and 2.14 ± .44 × 10{sup −3} mm{sup 2}/s, P = 87, respectively). T2 shine-through effect was less frequently observed in cirrhosis (P = .02). Conclusion: MR imaging characteristics of hepatic hemangioma were similar in patients with normal compared to fibrotic and cirrhotic livers. Smaller lesion size was observed with liver disease and less T2 shine-through effect was seen in

  20. Effect of regional lung inflation on ventilation heterogeneity at different length scales during mechanical ventilation of normal sheep lungs.

    Science.gov (United States)

    Wellman, Tyler J; Winkler, Tilo; Costa, Eduardo L V; Musch, Guido; Harris, R Scott; Venegas, Jose G; Vidal Melo, Marcos F

    2012-09-01

    Heterogeneous, small-airway diameters and alveolar derecruitment in poorly aerated regions of normal lungs could produce ventilation heterogeneity at those anatomic levels. We modeled the washout kinetics of (13)NN with positron emission tomography to examine how specific ventilation (sV) heterogeneity at different length scales is influenced by lung aeration. Three groups of anesthetized, supine sheep were studied: high tidal volume (Vt; 18.4 ± 4.2 ml/kg) and zero end-expiratory pressure (ZEEP) (n = 6); low Vt (9.2 ± 1.0 ml/kg) and ZEEP (n = 6); and low Vt (8.2 ± 0.2 ml/kg) and positive end-expiratory pressure (PEEP; 19 ± 1 cmH(2)O) (n = 4). We quantified fractional gas content with transmission scans, and sV with emission scans of infused (13)NN-saline. Voxel (13)NN-washout curves were fit with one- or two-compartment models to estimate sV. Total heterogeneity, measured as SD[log(10)(sV)], was divided into length-scale ranges by measuring changes in variance of log(10)(sV), resulting from progressive filtering of sV images. High-Vt ZEEP showed higher sV heterogeneity at 36-mm (r = -0.72) length scales (P < 0.001). We conclude that sV heterogeneity at length scales <60 mm increases in poorly aerated regions of mechanically ventilated normal lungs, likely due to heterogeneous small-airway narrowing and alveolar derecruitment. PEEP reduces sV heterogeneity by maintaining lung expansion and airway patency at those small length scales.

  1. Fracture resistance of structurally compromised and normal endodontically treated teeth restored with different post systems: An in vitro study

    Directory of Open Access Journals (Sweden)

    Vajihesadat Mortazavi

    2012-01-01

    Full Text Available Background: With the aim of developing methods that could increase the fracture resistance of structurally compromised endodontically treated teeth, this study was conducted to compare the effect of three esthetic post systems on the fracture resistance and failure modes of structurally compromised and normal roots. Materials and Methods: Forty five extracted and endodontically treated maxillary central teeth were assigned to 5 experimental groups (n=9. In two groups, the post spaces were prepared with the corresponding drills of the post systems to be restored with double taper light posts (DT.Light-Post (group DT.N and zirconia posts (Cosmopost (group Zr.N. In other 3 groups thin wall canals were simulated to be restored with Double taper Light posts (DT.W, double taper Light posts and Ribbond fibers (DT+R.W and Zirconia posts (Zr.W. After access cavity restoration and thermocycling, compressive load was applied and the fracture strength values and failure modes were evaluated. Data were analyzed using two-way ANOVA, Tukey and Fisher exact tests (P<0.05. Results: The mean failure loads (N were 678.56, 638.22, 732.44, 603.44 and 573.67 for groups DT.N, Zr.N, DT.W, DT+R.W and Zr.w respectively. Group DT+R.W exhibited significantly higher resistance to fracture compared to groups Zr.N, DT.W and Zr.w (P<0.05. A significant difference was detected between groups DT.N and Zr.W (P=0.027. Zirconia posts showed significantly higher root fracture compared to fiber posts (P=0.004. Conclusion: The structurally compromised teeth restored with double taper light posts and Ribbond fibers showed the most fracture resistance and their strengths were comparable to those of normal roots restored with double taper light posts. More desirable fracture patterns were observed in teeth restored with fiber posts.

  2. Canine epidermal lipid sampling by skin scrub revealed variations between different body sites and normal and atopic dogs.

    Science.gov (United States)

    Angelbeck-Schulze, Mandy; Mischke, Reinhard; Rohn, Karl; Hewicker-Trautwein, Marion; Naim, Hassan Y; Bäumer, Wolfgang

    2014-07-10

    Previously, we evaluated a minimally invasive epidermal lipid sampling method called skin scrub, which achieved reproducible and comparable results to skin scraping. The present study aimed at investigating regional variations in canine epidermal lipid composition using the skin scrub technique and its suitability for collecting skin lipids in dogs suffering from certain skin diseases. Eight different body sites (5 highly and 3 lowly predisposed for atopic lesions) were sampled by skin scrub in 8 control dogs with normal skin. Additionally, lesional and non-lesional skin was sampled from 12 atopic dogs and 4 dogs with other skin diseases by skin scrub. Lipid fractions were separated by high performance thin layer chromatography and analysed densitometrically. No significant differences in total lipid content were found among the body sites tested in the control dogs. However, the pinna, lip and caudal back contained significantly lower concentrations of ceramides, whereas the palmar metacarpus and the axillary region contained significantly higher amounts of ceramides and cholesterol than most other body sites. The amount of total lipids and ceramides including all ceramide classes were significantly lower in both lesional and non-lesional skin of atopic dogs compared to normal skin, with the reduction being more pronounced in lesional skin. The sampling by skin scrub was relatively painless and caused only slight erythema at the sampled areas but no oedema. Histological examinations of skin biopsies at 2 skin scrubbed areas revealed a potential lipid extraction from the transition zone between stratum corneum and granulosum. The present study revealed regional variations in the epidermal lipid and ceramide composition in dogs without skin abnormalities but no connection between lipid composition and predilection sites for canine atopic dermatitis lesions. The skin scrub technique proved to be a practicable sampling method for canine epidermal lipids, revealed

  3. What’s Normal? Immune Profiling of Human Milk from Healthy Women Living in Different Geographical and Socioeconomic Settings

    Science.gov (United States)

    Ruiz, Lorena; Espinosa-Martos, Irene; García-Carral, Cristina; Manzano, Susana; McGuire, Michelle K.; Meehan, Courtney L.; McGuire, Mark A.; Williams, Janet E.; Foster, James; Sellen, Daniel W.; Kamau-Mbuthia, Elizabeth W.; Kamundia, Egidioh W.; Mbugua, Samwel; Moore, Sophie E.; Kvist, Linda J.; Otoo, Gloria E.; Lackey, Kimberly A.; Flores, Katherine; Pareja, Rossina G.; Bode, Lars; Rodríguez, Juan M.

    2017-01-01

    Human milk provides a very wide range of nutrients and bioactive components, including immune factors, human milk oligosaccharides, and a commensal microbiota. These factors are essential for interconnected processes including immunity programming and the development of a normal infant gastrointestinal microbiome. Newborn immune protection mostly relies on maternal immune factors provided through milk. However, studies dealing with an in-depth profiling of the different immune compounds present in human milk and with the assessment of their natural variation in healthy women from different populations are scarce. In this context, the objective of this work was the detection and quantification of a wide array of immune compounds, including innate immunity factors (IL1β, IL6, IL12, INFγ, TNFα), acquired immunity factors (IL2, IL4, IL10, IL13, IL17), chemokines (IL8, Groα, MCP1, MIP1β), growth factors [IL5, IL7, epidermal growth factor (EGF), granulocyte colony-stimulating factor, granulocyte–macrophage colony-stimulating factor, TGFβ2], and immunoglobulins (IgA, IgG, IgM), in milk produced by healthy women of different ethnicities living in different geographic, dietary, socioeconomic, and environmental settings. Among the analyzed factors, IgA, IgG, IgM, EGF, TGFβ2, IL7, IL8, Groα, and MIP1β were detected in all or most of the samples collected in each population and, therefore, this specific set of compounds might be considered as the “core” soluble immune factors in milk produced by healthy women worldwide. This approach may help define which immune factors are (or are not) common in milk produced by women living in various conditions, and to identify host, lifestyle, and environmental factors that affect the immunological composition of this complex biological fluid. Clinical Trial Registration: www.ClinicalTrials.gov, identifier NCT02670278. PMID:28713365

  4. What’s Normal? Immune Profiling of Human Milk from Healthy Women Living in Different Geographical and Socioeconomic Settings

    Directory of Open Access Journals (Sweden)

    Lorena Ruiz

    2017-06-01

    Full Text Available Human milk provides a very wide range of nutrients and bioactive components, including immune factors, human milk oligosaccharides, and a commensal microbiota. These factors are essential for interconnected processes including immunity programming and the development of a normal infant gastrointestinal microbiome. Newborn immune protection mostly relies on maternal immune factors provided through milk. However, studies dealing with an in-depth profiling of the different immune compounds present in human milk and with the assessment of their natural variation in healthy women from different populations are scarce. In this context, the objective of this work was the detection and quantification of a wide array of immune compounds, including innate immunity factors (IL1β, IL6, IL12, INFγ, TNFα, acquired immunity factors (IL2, IL4, IL10, IL13, IL17, chemokines (IL8, Groα, MCP1, MIP1β, growth factors [IL5, IL7, epidermal growth factor (EGF, granulocyte colony-stimulating factor, granulocyte–macrophage colony-stimulating factor, TGFβ2], and immunoglobulins (IgA, IgG, IgM, in milk produced by healthy women of different ethnicities living in different geographic, dietary, socioeconomic, and environmental settings. Among the analyzed factors, IgA, IgG, IgM, EGF, TGFβ2, IL7, IL8, Groα, and MIP1β were detected in all or most of the samples collected in each population and, therefore, this specific set of compounds might be considered as the “core” soluble immune factors in milk produced by healthy women worldwide. This approach may help define which immune factors are (or are not common in milk produced by women living in various conditions, and to identify host, lifestyle, and environmental factors that affect the immunological composition of this complex biological fluid.Clinical Trial Registration:www.ClinicalTrials.gov, identifier NCT02670278.

  5. Virus expression in different tissues of normal and tumor-bearing mice inoculated with a murine leukemia virus.

    Science.gov (United States)

    Youn, J K; Santillana, M; Hue, G; Barski, G

    1977-11-15

    Evolution of virus expression in different lymphoid organs as well as in solid syngeneic tumors of mice inoculated with an MuLV was studied with the aid of in vitro XC co-culture technique. When normal adult mice of strain XLII were inoculated intraperitoneally with a cultured Rauscher virus (RC), the virus could be detected, 10 days after inoculation, only in bone marrow in small amounts and thereafter no virus could be found in any of the organs tested, including bone marrow, spleen, thymus, lymph node and kidney. However, when age- and sex-matched parallel mice bearing syngeneic subcutaneous non-viral tumors were inoculated similarly with the RC virus, the virus could be detected abundantly not only in bone marrow and spleen but also in tumors during the first 3 weeks and even 6 weeks after virus inoculation. Transitional decrease or disappearance of the virus was observed around the 25th-31st day in organs and tumors of the inoculated mice. When the tumor mass was removed from these mice by surgery, the virus disappeared rapidly and definitely from all the organs tested. The virus recovered from in vitro explanted and cultured tumors, taken from mice inoculated with the virus, induced typical lymphoid leukemia in BALB/c mice inoculated as newborns. However, from certain aspects (hypertrophy of the thymus and lymph nodes), this virus was different from the original RC virus.

  6. Effects of Normalized Difference Vegetation Index and Related Wavebands' Characteristics on Detecting Spatial Heterogeneity Using Variogram-based Analysis

    Institute of Scientific and Technical Information of China (English)

    WEN Zhaofei; ZHANG Ce; ZHANG Shuqing; DING Changhong; LIU Chunyue; PAN Xin; LI Huapeng; SUN Yan

    2012-01-01

    Spatial heterogeneity is widely used in diverse applications,such as recognizing ecological process,guiding ecological restoration,managing land use,etc.Many researches have focused on the inherent scale multiplicity of spatial heterogeneity by using various environmental variables.How these variables affect their corresponding spatial heterogeneities,however,have received little attention.In this paper,we examined the effects of characteristics of normalized difference vegetation index (NDVI) and its related bands variable images,namely red and near infrared (NIR),on their corresponding spatial heterogeneity detection based on variogram models.In a coastal wetland region,two groups of study sites with distinct fractal vegetation cover were tested and analyzed.The results show that:1) in high fractal vegetation cover (H-FVC) area,NDVI and NIR variables display a similar ability in detecting the spatial heterogeneity caused by vegetation growing status structure; 2) in low fractal vegetation cover (L-FVC) area,the NIR and red variables outperform NDVI in the survey of soil spatial heterogeneity; and 3) generally,NIR variable is ubiquitously applicable for vegetation spatial heterogeneity investigation in different fractal vegetation covers.Moreover,as variable selection for remote sensing applications should fully take the characteristics of variables and the study object into account,the proposed variogram analysis method can make the variable selection objectively and scientifically,especially in studies related to spatial heterogeneity using remotely sensed data.

  7. Interannual Variability of the Normalized Difference Vegetation Index on the Tibetan Plateau and Its Relationship with Climate Change

    Institute of Scientific and Technical Information of China (English)

    ZHOU Dingwen; FAN Guangzhou; HUANG Ronghui; FANG Zhifang; LIU Yaqin; LI Hongquan

    2007-01-01

    The Qinghai-Xizang Plateau, or Tibetan Plateau, is a sensitive region for climate change, where the manifestation of global warming is particularly noticeable. The wide climate variability in this region significantly affects the local land ecosystem and could consequently lead to notable vegetation changes. In this paper, the interannual variations of the plateau vegetation are investigated using a 21-year normalized difference vegetation index (NDVI) dataset to quantify the consequences of climate warming for the regional ecosystem and its interactions. The results show that vegetation coverage is best in the eastern and southern plateau regions and deteriorates toward the west and north. On the whole, vegetation activity demonstrates a gradual enhancement in an oscillatory manner during 1982-2002. The temporal variation also exhibits striking regional differences: an increasing trend is most apparent in the west, south, north and southeast, whereas a decreasing trend is present along the southern plateau boundary and in the central-east region. Covariance analysis between the NDVI and surface temperature/precipitation suggests that vegetation change is closely related to climate change. However, the controlling physical processes vary geographically. In the west and east, vegetation variability is found to be driven predominantly by temperature, with the impact of precipitation being of secondary importance. In the central plateau, however, temperature and precipitation factors are equally important in modulating the interannual vegetation variability.

  8. NDVI (Normalized Difference Vegetation Index) signatures of transient ecohydrological systems: The case of post-mining landscapes

    Science.gov (United States)

    Brück, Yasemine; Schulte Overberg, Philipp; Pohle, Ina; Hinz, Christoph

    2017-04-01

    Assessing ecohydrological systems that undergo state transitions due to environmental change is becoming increasingly important. One system that can be used to study severe disturbances are post-mining landscapes as they usually are associated with complete removal of vegetation and afterwards subsequent ecosystem restoration or spontaneous rehabilitation in line with natural succession. Within this context it is of interest, whether and how (fast) the land cover in these areas returns to conditions comparable to those in the undisturbed surrounding or those prior mining. Many aspects of mine site rehabilitation depend on climatic, geomorphic and ecological settings, which determine at which rate vegetation may be re-established. In order to identify general patterns of vegetation establishment, we propose to use NDVI (Normalized Difference Vegetation Index) time series for mine affected land to estimate rate of recovery across climate regions and ecoregions. In this study we analysed the MODIS Terra Satellite 8 day-composite NDVI for areas influenced by surface mining in different climates from 2001 to 2015. The locations have been chosen based on their extent and the data availability of mining and rehabilitation activities. We selected coal extraction as a case study as strip mining generates well-defined chronosequences of disturbance. The selected mining areas are located in equatorial, arid, warm temperate or snow climates with different precipitation and temperature conditions according to the Köppen-Geiger classification. We analysed the NDVI time series regarding significant characteristics of the re-vegetation phase. We applied hierarchical cluster analysis to capture the spatial heterogeneity between different pixels (ca. 250 * 250 m2 each) in and around each open cast mine. We disentangled seasonality, trend and residual components in the NDVI time series by Seasonal and Trend decomposition using LOESS. As expected the time of the removal of vegetation

  9. Differences in lifestyle behaviors, dietary habits, and familial factors among normal-weight, overweight, and obese Chinese children and adolescents

    Directory of Open Access Journals (Sweden)

    Guo Xiaofan

    2012-10-01

    Full Text Available Abstract Background Pediatric obesity has become a global public health problem. Data on the lifestyle behaviors, dietary habits, and familial factors of overweight and obese children and adolescents are limited. The present study aims to compare health-related factors among normal-weight, overweight, and obese Chinese children and adolescents. Methods We conducted a cross-sectional study consisted of 4262 children and adolescents aged 5–18 years old from rural areas of the northeast China. Anthropometric measurements and self-reported information on health-related variables, such as physical activities, sleep duration, dietary habits, family income, and recognition of weight status from the views of both children and parents, were collected by trained personnel. Results The prevalence rates of overweight and obesity were 15.3 and 6.4%, respectively. Compared to girls, boys were more commonly overweight (17.5% vs. 12.9% and obese (9.5% vs. 3.1%. Approximately half of the parents with an overweight or obese child reported that they failed to recognize their child’s excess weight status, and 65% of patients with an overweight child reported that they would not take measures to decrease their child’s body weight. Obese children and adolescents were more likely to be nonsnackers [odds ratio (OR: 1.348; 95% confidence interval (CI: 1.039–1.748] and to have a family income of 2000 CNY or more per month (OR: 1.442; 95% CI: 1.045–1.99 and less likely to sleep longer (≥7.5 h (OR: 0.475; 95% CI: 0.31–0.728 than the normal-weight participants. Conclusions Our study revealed a high prevalence of overweight and obesity in a large Chinese pediatric population. Differences in sleep duration, snacking, family income, and parental recognition of children’s weight status among participants in different weight categories were observed, which should be considered when planning prevention and treatment programs for pediatric obesity.

  10. Reference genes selection and normalization of oxidative stress responsive genes upon different temperature stress conditions in Hypericum perforatum L.

    Science.gov (United States)

    Velada, Isabel; Ragonezi, Carla; Arnholdt-Schmitt, Birgit; Cardoso, Hélia

    2014-01-01

    Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization. Hypericum perforatum L. (St. John's wort) is a field growing plant that is frequently exposed to a variety of adverse environmental stresses that can negatively affect its productivity. This widely known medicinal plant with broad pharmacological properties (anti-depressant, anti-tumor, anti-inflammatory, antiviral, antioxidant, anti-cancer, and antibacterial) has been overlooked with respect to the identification of reference genes suitable for RT-qPCR data normalization. In this study, 11 candidate reference genes were analyzed in H. perforatum plants subjected to cold and heat stresses. The expression stability of these genes was assessed using GeNorm, NormFinder and BestKeeper algorithms. The results revealed that the ranking of stability among the three algorithms showed only minor differences within each treatment. The best-ranked reference genes differed between cold- and heat-treated samples; nevertheless, TUB was the most stable gene in both experimental conditions. GSA and GAPDH were found to be reliable reference genes in cold-treated samples, while GAPDH showed low expression stability in heat-treated samples. 26SrRNA and H2A had the highest stabilities in the heat assay, whereas H2A was less stable in the cold assay. Finally, AOX1, AOX2, CAT1 and CHS genes, associated with plant stress responses and oxidative stress, were used as target genes to validate the reliability of identified reference genes. These target genes showed differential expression profiles over time in treated samples. This study not only is the first systematic analysis for the selection of suitable reference genes for RT-qPCR studies in H. perforatum subjected to temperature stress conditions, but may also provide valuable information

  11. Reference genes selection and normalization of oxidative stress responsive genes upon different temperature stress conditions in Hypericum perforatum L.

    Directory of Open Access Journals (Sweden)

    Isabel Velada

    Full Text Available Reverse transcription-quantitative real-time PCR (RT-qPCR is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization. Hypericum perforatum L. (St. John's wort is a field growing plant that is frequently exposed to a variety of adverse environmental stresses that can negatively affect its productivity. This widely known medicinal plant with broad pharmacological properties (anti-depressant, anti-tumor, anti-inflammatory, antiviral, antioxidant, anti-cancer, and antibacterial has been overlooked with respect to the identification of reference genes suitable for RT-qPCR data normalization. In this study, 11 candidate reference genes were analyzed in H. perforatum plants subjected to cold and heat stresses. The expression stability of these genes was assessed using GeNorm, NormFinder and BestKeeper algorithms. The results revealed that the ranking of stability among the three algorithms showed only minor differences within each treatment. The best-ranked reference genes differed between cold- and heat-treated samples; nevertheless, TUB was the most stable gene in both experimental conditions. GSA and GAPDH were found to be reliable reference genes in cold-treated samples, while GAPDH showed low expression stability in heat-treated samples. 26SrRNA and H2A had the highest stabilities in the heat assay, whereas H2A was less stable in the cold assay. Finally, AOX1, AOX2, CAT1 and CHS genes, associated with plant stress responses and oxidative stress, were used as target genes to validate the reliability of identified reference genes. These target genes showed differential expression profiles over time in treated samples. This study not only is the first systematic analysis for the selection of suitable reference genes for RT-qPCR studies in H. perforatum subjected to temperature stress conditions, but may also provide

  12. Normalized Difference Vegetation Index as a Tool for Wheat Yield Estimation: A Case Study from Faisalabad, Pakistan

    Directory of Open Access Journals (Sweden)

    Syeda Refat Sultana

    2014-01-01

    Full Text Available For estimation of grain yield in wheat, Normalized Difference Vegetation Index (NDVI is considered as a potential screening tool. Field experiments were conducted to scrutinize the response of NDVI to yield behavior of different wheat cultivars and nitrogen fertilization at agronomic research area, University of Agriculture Faisalabad (UAF during the two years 2008-09 and 2009-10. For recording the value of NDVI, Green seeker (Handheld-505 was used. Split plot design was used as experimental model in, keeping four nitrogen rates (N1= 0 kg ha−1, N2= 55 kg ha−1, N3=110 kg ha−1, and N4= 220 kg ha−1 in main plots and ten wheat cultivars (Bakkhar-2001, Chakwal-50, Chakwal-97, Faisalabad-2008, GA-2002, Inqlab-91, Lasani-2008, Miraj-2008, Sahar-2006, and Shafaq-2006 in subplots with four replications. Impact of nitrogen and difference between cultivars were forecasted through NDVI. The results suggested that nitrogen treatment N4 (220 kg ha−1 and cultivar Faisalabad-2008 gave maximum NDVI value (0.85 at grain filling stage among all treatments. The correlation among NDVI at booting, grain filling, and maturity stages with grain yield was positive (R2 = 0.90; R2 = 0.90; R2 = 0.95, respectively. So, booting, grain filling, and maturity can be good depictive stages during mid and later growth stages of wheat crop under agroclimatic conditions of Faisalabad and under similar other wheat growing environments in the country.

  13. [The effect of alloxan on the multiplication of different types of normal or cancerous cells, and on the transformation of chick embryo fibroblasts by Rous virus in vitro].

    Science.gov (United States)

    Grobon, P; Latarjet, R

    1975-05-26

    The action of alloxan was studied in vitro, on different categories of normal or cancerous cells. At concentrations of 250 and 350 gamma/ml, alloxan does not significantly inhibit the growth of normal cells, whereas it does inhibit the growth of cancerous cells. Furthermore, alloxan inhibits infection and transformation of chicken-embryo fibroblasts infected by the Rous virus.

  14. The Effect of Spinal Tap Test on Different Sensory Modalities of Postural Stability in Idiopathic Normal Pressure Hydrocephalus

    Science.gov (United States)

    Abram, Katrin; Bohne, Silvia; Bublak, Peter; Karvouniari, Panagiota; Klingner, Carsten M.; Witte, Otto W.; Guntinas-Lichius, Orlando; Axer, Hubertus

    2016-01-01

    Background/Aims Postural instability in patients with normal pressure hydrocephalus (NPH) is a most crucial symptom leading to falls with secondary complications. The aim of the current study was to evaluate the therapeutic effect of spinal tap on postural stability in these patients. Methods Seventeen patients with clinical symptoms of NPH were examined using gait scale, computerized dynamic posturography (CDP), and neuropsychological assessment. Examinations were done before and after spinal tap test. Results The gait score showed a significant improvement 24 h after spinal tap test in all subtests and in the sum score (p < 0.003), while neuropsychological assessment did not reveal significant differences 72 h after spinal tap test. CDP showed significant improvements after spinal tap test in the Sensory Organization Tests 2 (p = 0.017), 4 (p = 0.001), and 5 (p = 0.009) and the composite score (p = 0.01). Patients showed best performance in somatosensory and worst performance in vestibular dominated tests. Vestibular dominated tests did not improve significantly after spinal tap test, while somatosensory and visual dominated tests did. Conclusion Postural stability in NPH is predominantly affected by deficient vestibular functions, which did not improve after spinal tap test. Conditions which improved best were mainly independent from visual control and are based on proprioceptive functions. PMID:27790243

  15. Metabolomic analysis reveals differences in umbilical vein plasma metabolites between normal and growth-restricted fetal pigs during late gestation.

    Science.gov (United States)

    Lin, Gang; Liu, Chuang; Feng, Cuiping; Fan, Zhiyong; Dai, Zhaolai; Lai, Changhua; Li, Zhen; Wu, Guoyao; Wang, Junjun

    2012-06-01

    Intrauterine growth restriction (IUGR) remains a major problem for both human health and animal production due to its association with high rates of neonatal morbidity and mortality, low efficiency of food utilization, permanent adverse effects on postnatal growth and development, and long-term health and productivity of the offspring. However, the underlying mechanisms for IUGR are largely unknown. In this study, one IUGR fetus and one normal body weight (NBW) fetus were obtained from each of 9 gilts at each of 2 gestational ages (d 90 and 110). Metabolomes of umbilical vein plasma in IUGR and NBW fetuses were determined by MS, while hormones, amino acids, and related metabolites in maternal and fetal plasma were measured using assay kits and chromatographic methods. Metabolites (including glucose, urea, ammonia, amino acids, and lipids) in umbilical vein plasma exhibited a cluster of differences between IUGR and NBW fetuses on d 90 and 110 of gestation. These changes in the IUGR group are associated with disorders of nutrient and energy metabolism as well as endocrine imbalances, which may contribute to the retardation of fetal growth and development. The findings help provide information regarding potential mechanisms responsible for IUGR in swine and also have important implications for the design of effective strategies to prevent, diagnose, and treat IUGR in other mammalian species, including humans.

  16. Unmanned aircraft system-derived crop height and normalized difference vegetation index metrics for sorghum yield and aphid stress assessment

    Science.gov (United States)

    Stanton, Carly; Starek, Michael J.; Elliott, Norman; Brewer, Michael; Maeda, Murilo M.; Chu, Tianxing

    2017-04-01

    A small, fixed-wing unmanned aircraft system (UAS) was used to survey a replicated small plot field experiment designed to estimate sorghum damage caused by an invasive aphid. Plant stress varied among 40 plots through manipulation of aphid densities. Equipped with a consumer-grade near-infrared camera, the UAS was flown on a recurring basis over the growing season. The raw imagery was processed using structure-from-motion to generate normalized difference vegetation index (NDVI) maps of the fields and three-dimensional point clouds. NDVI and plant height metrics were averaged on a per plot basis and evaluated for their ability to identify aphid-induced plant stress. Experimental soil signal filtering was performed on both metrics, and a method filtering low near-infrared values before NDVI calculation was found to be the most effective. UAS NDVI was compared with NDVI from sensors onboard a manned aircraft and a tractor. The correlation results showed dependence on the growth stage. Plot averages of NDVI and canopy height values were compared with per-plot yield at 14% moisture and aphid density. The UAS measures of plant height and NDVI were correlated to plot averages of yield and insect density. Negative correlations between aphid density and NDVI were seen near the end of the season in the most damaged crops.

  17. Correlation of meteorological parameters and remotely sensed normalized difference vegetation index (NDVI) with cotton leaf curl virus (CLCV) in Multan

    Science.gov (United States)

    Ahmed, A.; Akhtar, A.; Khalid, B.; Shamim, A.

    2013-06-01

    Climate change and weather has a profound effect on the spread of Cotton Leaf Curl Virus (CLCV) which is transmitted by whitefly. Climate change is altering temperature and precipitation patterns, resulting in the shift of some insect/pest from small population to large population thus effecting crops yield. To find out the relationship between the weather conditions, outburst of CLCV and changes in Normalized Difference Vegetation Index (NDVI) values due to the outburst of CLCV, a study was carried out for tehsil Multan. Data was acquired for the months of June, July, August and September for the year 2010. Regression analysis between CLCV and meteorological conditions as well as between CLCV and NDVI was performed. Meteorological parameters included temperature, humidity, precipitation, cloud cover, wind direction, pan evaporation and sunshine hours. NDVI values were calculated from SPOT satellite imagery (1km) using ArcMap10 and WinDisp v5.1. Correlation coefficients obtained in most of the cases were acceptable however the significance F and P-value were higher than their critical value at 95% level of significance. Therefore significant correlation was found only between CLCV and temperature and between CLCV and PAN evaporation during the month of July.

  18. Stay-green in spring wheat can be determined by spectral reflectance measurements (normalized difference vegetation index) independently from phenology.

    Science.gov (United States)

    Lopes, Marta S; Reynolds, Matthew P

    2012-06-01

    The green area displayed by a crop is a good indicator of its photosynthetic capacity, while chlorophyll retention or 'stay-green' is regarded as a key indicator of stress adaptation. Remote-sensing methods were tested to estimate these parameters in diverse wheat genotypes under different growing conditions. Two wheat populations (a diverse set of 294 advanced lines and a recombinant inbred line population of 169 sister lines derived from the cross between Seri and Babax) were grown in Mexico under three environments: drought, heat, and heat combined with drought. In the two populations studied here, a moderate heritable expression of stay-green was found-when the normalized difference vegetation index (NDVI) at physiological maturity was estimated using the regression of NDVI over time from the mid-stages of grain-filling to physiological maturity-and for the rate of senescence during the same period. Under heat and heat combined with drought environments, stay-green calculated as NDVI at physiological maturity and the rate of senescence, showed positive and negative correlations with yield, respectively. Moreover, stay-green calculated as an estimation of NDVI at physiological maturity and the rate of senescence regressed on degree days give an independent measurement of stay-green without the confounding effect of phenology. On average, in both populations under heat and heat combined with drought environments CTgf and stay-green variables accounted for around 30% of yield variability in multiple regression analysis. It is concluded that stay-green traits may provide cumulative effects, together with other traits, to improve adaptation under stress further.

  19. Subsidence hazard and risk assessments for Mexico City: An interdisciplinary analysis of satellite-derived subsidence map (PSInSAR) and census data.

    Science.gov (United States)

    Solano Rojas, D. E.; Cabral-Cano, E.; Wdowinski, S.; Hernaández Espriú, A.; Falorni, G.; Bohane, A.

    2014-12-01

    The Mexico City Metropolitan Area is the largest urban center in the American continent, with 20.4 millions of inhabitants, representing 17.8% of the total population of the country. Over the past several decades Mexico City has been experienced rapid subsidence, up to ~370 mm/yr, caused by groundwater extraction. The subsidence rate is inhomogeneous, as it controlled by the local geology. Unconsolidated sediments tend to compact and induce rapid subsidence, whereas subsurface volcanic rocks are less prone to subsidence. Intensive faulting in the city has been observed in areas of differential deformation; in these areas buildings and infrastructure are highly damaged. Quantification of subsidence-induce damage is needed for establishing the magnitude of the phenomenon. Our study uses three data sources: a satellite-derived subsidence map, census information of population distribution for 2010, and information on buildings and infrastructure. The subsidence map was calculated from 29 SAR scene acquired by the Envisat satellite during the years 2003-2010 using the Persistent Scatterers Interferometry (PSI) method with the SqueeSAR algorithm. The information of the census of population comes from the National Institute of Statistics and Geography (INEGI), which also provides the information about infrastructure. We intersected the information from the three maps using a geographic information system (GIS), which cover an area of 1, 640 km2. As subsidence-induced damage occurs mainly in areas of differential subsidence, we based the GIS analysis on the subsidence gradients, rather than subsidence rates. In order to evaluate subsidence-induced faulting risk, we generated a risk matrix that worked as the main parameter to create a risk map. We then reclassified the urban area into 5 zones according to the related risk, with R0 for the lowest risk and R4 for the highest. Our counting showed that 350 km2 of the city is located in an urban area of high to very high risk

  20. The Greenhouse Gas Climate Change Initiative (GHG-CCI): comparison and quality assessment of near-surface-sensitive satellite-derived CO2 and CH4 global data sets

    Science.gov (United States)

    Buchwitz, Michael

    2013-04-01

    The GHG-CCI project is one of several projects of the European Space Agency's (ESA) Climate Change Initiative (CCI). The goal of the CCI is to generate and deliver data sets of various satellite-derived Essential Climate Variables (ECVs) in line with GCOS (Global Climate Observing System) requirements. The "ECV Greenhouse Gases" (ECV GHG) is the global distribution of important climate relevant gases - atmospheric CO2 and CH4 - with a quality sufficient to obtain information on regional CO2 and CH4 sources and sinks. Two satellite instruments deliver the main input data for GHG-CCI: SCIAMACHY/ENVISAT and TANSO-FTS/GOSAT. The first order priority goal of GHG-CCI is the further development of retrieval algorithms for near-surface-sensitive column-averaged dry air mole fractions of CO2 and CH4, denoted XCO2 and XCH4, to meet the demanding user requirements. GHG-CCI focusses on four core data products: XCO2 from SCIAMACHY and TANSO and XCH4 from the same two sensors. For each of the four core data products at least two candidate retrieval algorithms have been independently further developed and the corresponding data products have been quality assessed and inter-compared. This activity is referred to as "Round Robin" (RR) activity within the CCI. The main goal of the RR was to identify for each of the four core products which algorithm to be used to generate the Climate Research Data Package (CRDP), which will essentially be the first version of the ECV GHG. This manuscript gives an overview about the GHG-CCI RR and related activities. This comprises the establishment of the user requirements, the improvement of the candidate retrieval algorithms and comparisons with ground-based observations and models. The manuscript summarizes the final RR algorithm selection decision and its justification. Comparison with ground-based Total Carbon Column Observing Network (TCCON) data indicates that the "breakthrough" single measurement precision requirement has been met for

  1. Microvessel density at different levels of normal or injured bile duct in dogs and its surgical implications

    Institute of Scientific and Technical Information of China (English)

    LinGeng; DingLuo; Hao-ChunZhang; Liang-SongZhao; De-KeQing

    2011-01-01

    BACKGROUND: Ischemic recurrent stricture after surgical repair for iatrogenic bile duct injury (BDI) remains a challenge in clinical practice. The present study was designed to investigate whether ischemia is universal and of varied severity at different levels of the proximal bile duct after BDI. METHODS: A total of 30 beagle dogs were randomly divided into control, BDI, and BDI-repaired groups. The BDI animal model was established based on the classic pattern of laparoscopic cholecystectomy-related BDI. The animals were sacrificed on postoperative day 15, and bile duct tissue was harvested to assess microvessel density (MVD) at selected levels of the normal, post-BDI and BDI-repaired bile duct with the CD34 immunohistochemistry technique. RESULTS:  In the control group, MVD at level H (high level) was remarkably higher than that at level L (low level). No significant difference was found between MVDs at levels H and M (middle level), as well as at levels M and L. However, the tendency was noted that the closer the level to the hilus, the greater the MVD at that level. In both the BDI and BDI-repaired groups, MVDs at level H were generally greater than those at level L, despite the unremarkable differences between MVDs at neighboring levels. In these two groups, a similar tendency of MVD distribution to that in the control group was found; the closer the level to the injury site, the lower was the MVD at that level. Moreover, compared with the MDVs at the levels M and L in the control group, MVDs at the corresponding levels in the BDI and BDI-repaired groups were all remarkably reduced (P CONCLUSIONS: After BDI, universal ischemic damage in the injured proximal bile duct develops close to the injury site, while close to the hilus, ischemia is relatively slight. High hepaticojejunostomy, rather than low biloenterostomy or end-to-end duct anastomosis, should be recommended for BDI repair. Great care should be taken to protect the peribiliary plexus during

  2. A comparison with result of normalized image to different template image on statistical parametric mapping of ADHD children patients

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Dong Ho [Kyonggi University, Suwon (Korea, Republic of); Park, Soung Ock; Kwon, Soo Il [Dongnam Health College, Suwon (Korea, Republic of); Joh, Chol Woo; Yoon, Seok Nam [Medical College, Ajou University, Suwon (Korea, Republic of)

    2003-06-15

    We studied 64 ADHD children patients group (4 {approx} 15 ys, mean age: 8 {+-} 2.6 ys. M/F: 52/12) and 12 normal group (6 {approx} 7 ys, mean age: 9.4 {+-} 3.4 ys, M/F: 8/4) of the brain had been used to analysis of blood flow between normal and ADHD group. For analysis of Children ADHD, we used 12 children's mean brain images and made Template image of SPM99 program. In crease of blood flow (P-value 0.05), the result of normalized images to Template image to offer from SPM99 program, showed significant cluster in inter-Hemispheric and occipital Lobe, in the case of normalized images to children template image, showed inter-hemispheric and parietal lobe.

  3. Intraorgan differences of blood flow, oxygen supply and glycogen content in the multilobular liver of normal and hemorrhagic rats.

    Science.gov (United States)

    Metzger, H P; Schywalsky, M

    1992-02-01

    In order to characterize intraorgan differences in blood supply of the rat liver, hepatic blood flow (HBF), surface oxygen tension (sPO2) and glycogen content of the largest and smallest lobi have been determined for normal and hemorrhagic rats (N = 68) in ketamin-xylazine anesthesia. 1. Mean HBF +/- SD of lobus sinister measured 1.07 +/- 0.23 ml/g min (n = 119 determinations, N = 9 rats); HBF of lob. caudatus dexter showed a left-shifted histogram (mean value = 0.77 ml/g.min, median = 0.72 ml/g.min, modul = 0.63 ml/g.min, p less than 0.005). 2. Mean sPO2 +/- SD of lob. sin. measured 23 +/- 6.8 mm Hg (n = 168, N = 16). The histograms of lob. caudat. dext. and sin. were left-shifted (mean value of l.c.d. = 9 mm Hg, median = 4 mm Hg, modul = 0 mm Hg, mean value of l.c.s. = 16 mm Hg, median = 17 mm Hg, modul = 0 mm Hg). Under hemorrhage sPO2 became almost zero in 91% of the measurements. 3. In response to an arterial bolus of fluorescence stained gamma-globulins, spreading of the dye showed a pronounced front and marked periportal area within lob. sin., while an irregular convective front and a much smaller area were detected within both of the lobi caudati. Under hemorrhage, intersinusoidal staining and undefined, irregular contours were observed within all lobes. 4. Compared with lob. sin. preferential glycogen depletion and partial centrilobular necrosis were detected within both of the lob. caudati while under hemorrhage the glycogen stores were empty and severe group necroses have been observed especially within the small lobi. From the data it is concluded that in comparison to lob. sin. an insufficient supply and pronounced vulnerability against hepatic ischemia exists within the small lobi caudati.

  4. Loss of surface horizon of an irrigated soil detected by radiometric images of normalized difference vegetation index.

    Science.gov (United States)

    Fabian Sallesses, Leonardo; Aparicio, Virginia Carolina; Costa, Jose Luis

    2017-04-01

    The use of the soil in the Humid Pampa of Argentina has changed since the mid-1990s from agricultural-livestock production (that included pastures with direct grazing) to a purely agricultural production. Also, in recent years the area under irrigation by central pivot has been increased to 150%. The waters used for irrigation are sodium carbonates. The combination of irrigation and rain increases the sodium absorption ratio of soil (SARs), consequently raising the clay dispersion and reducing infiltration. This implies an increased risk of soil loss. A reduction in the development of white clover crop (Trifolium repens L.) was observed at an irrigation plot during 2015 campaign. The clover was planted in order to reduce the impact of two maize (Zea mays L.) campaigns under irrigation, which had increased soil SAR and deteriorated soil structure. SPOT-5 radiometric normalized difference vegetation index (NDVI) images were used to determine two zones of high and low production. In each zone, four random points were selected for further geo-referenced field sampling. Two geo-referenced measures of effective depth and surface soil sampling were carried out in each point. Texture of soil samples was determined by Pipette Method of Sedimentation Analysis. Data exploratory analysis showed that low production zone had a media effective depth = 80 cm and silty clay loam texture, while high production zone had a media effective depth > 140 cm and silt loam texture. The texture class of the low production zone did not correspond to prior soil studies carried out by the INTA (National Institute of Agricultural Technology), which showed that those soil textures were silt loam at surface and silty clay loam at sub-surface. The loss of the A horizon is proposed as a possible explanation, but further research is required. Besides, the need of a soil cartography actualization, which integrates new satellite imaging technologies and geo-referenced measurements with soil sensors is

  5. Correlation between normalized difference vegetation index and malaria in a subtropical rain forest undergoing rapid anthropogenic alteration.

    Science.gov (United States)

    Wayant, Nicole M; Maldonado, Diego; Rojas de Arias, Antonieta; Cousiño, Blanca; Goodin, Douglas G

    2010-05-01

    Time-series of coarse-resolution greenness values derived through remote sensing have been used as a surrogate environmental variable to help monitor and predict occurrences of a number of vector-borne and zoonotic diseases, including malaria. Often, relationships between a remotely-sensed index of greenness, e.g. the normalized difference vegetation index (NDVI), and disease occurrence are established using temporal correlation analysis. However, the strength of these correlations can vary depending on type and change of land cover during the period of record as well as inter-annual variations in the climate drivers (precipitation, temperature) that control the NDVI values. In this paper, the correlation between a long (260 months) time-series of monthly disease case rates and NDVI values derived from the Global Inventory Modeling and Mapping Studies (GIMMS) data set were analysed for two departments (administrative units) located in the Atlantic Forest biome of eastern Paraguay. Each of these departments has undergone extensive deforestation during the period of record and our analysis considers the effect on correlation of active versus quiescent periods of case occurrence against a background of changing land cover. Our results show that timeseries data, smoothed using the Fourier Transform tool, showed the best correlation. A moving window analysis suggests that four years is the optimum time frame for correlating these values, and the strength of correlation depends on whether it is an active or a quiescent period. Finally, a spatial analysis of our data shows that areas where land cover has changed, particularly from forest to non-forest, are well correlated with malaria case rates.

  6. Mild cognitive disorders are associated with different patterns of brain asymmetry than normal ageing: the PATH through life study

    Directory of Open Access Journals (Sweden)

    Nicolas Cherbuin

    2010-05-01

    Full Text Available Background and Purpose: Defining how brain structures differ in pre-clinical dementia is important to better understand the pathological processes involved and to inform clinical practice. The aim of this study was to identify significant brain correlates (volume and asymmetry in volume of mild cognitive disorders when compared to normal controls in a large community-based sample of young-old individuals who were assessed for cognitive impairment. Methods: Cortical and sub-cortical volumes were measured using a semi-automated method in 398 participants aged 65-70 years who were selected from a larger randomly sampled cohort and who agreed to undergo an MRI scan. Diagnoses were reached based on established protocols for MCI and a more inclusive category of any Mild Cognitive Disorder (any-MCD: which includes AAMI, AACD, OCD, MNC, CDR, MCI. Logistic regression analyses were used to assess the relationship between volume and asymmetry of theoretically relevant cerebral structures (predictors and MCI or any-MCD while controlling for age, sex, and intra-cranial volume. Results: The main correlates of cognitive impairment assessed in multivariate analyses were hippocampal asymmetry (more to left, MCI: OR 0.83, 95%CI 0.71-0.96, p = .013; MCD: OR 0.86, 95%CI 0.77-0.97, p = .011, lateral ventricle asymmetry (more to left, MCI: OR 0.95, 95%CI 0.91-0.99, p = .009; MCD: OR 0.95, 95%CI 0.92-0.98, p = .004, and cerebellar cortex asymmetry (more to left, MCI: OR 1.51, 95%CI 1.13-2.01, p = .005. Conclusions: In this population-based cohort stronger associations were found between asymmetry measures, rather than raw volumes in cerebral structures, and mild cognitive disorders.

  7. A Comparison of Land Surface Water Mapping Using the Normalized Difference Water Index from TM, ETM+ and ALI

    Directory of Open Access Journals (Sweden)

    Bingyu Sun

    2013-10-01

    Full Text Available Remote sensing has more advantages than the traditional methods of land surface water (LSW mapping because it is a low-cost, reliable information source that is capable of making high-frequency and repeatable observations. The normalized difference water indexes (NDWIs, calculated from various band combinations (green, near-infrared (NIR, or shortwave-infrared (SWIR, have been successfully applied to LSW mapping. In fact, new NDWIs will become available when Advanced Land Imager (ALI data are used as the ALI sensor provides one green band (Band 4, two NIR bands (Bands 6 and 7, and three SWIR bands (Bands 8, 9, and 10. Thus, selecting the optimal band or combination of bands is critical when ALI data are employed to map LSW using NDWI. The purpose of this paper is to find the best performing NDWI model of the ALI data in LSW map. In this study, eleven NDWI models based on ALI, Thematic Mapper (TM, and Enhanced Thematic Mapper Plus (ETM+ data were compared to assess the performance of ALI data in LSW mapping, at three different study sites in the Yangtze River Basin, China. The contrast method, Otsu method, and confusion matrix were calculated to evaluate the accuracies of the LSW maps. The accuracies of LSW maps derived from eleven NDWI models showed that five NDWI models of the ALI sensor have more than an overall accuracy of 91% with a Kappa coefficient of 0.78 of LSW maps at three test sites. In addition, the NDWI model, calculated from the green (Band 4: 0.525–0.605 μm and SWIR (Band 9: 1.550–1.750 μm bands of the ALI sensor, namely NDWIA4,9, was shown to have the highest LSW mapping accuracy, more than the other NDWI models. Therefore, the NDWIA4,9 is the best indicator for LSW mapping of the ALI sensor. It can be used for mapping LSW with high accuracy.

  8. Normal values of flash visual evoked potentials in rabbits:Differences between the sexes and the eyes

    Institute of Scientific and Technical Information of China (English)

    Bo Bu; Dingbiao Zhou; Bainan Xu; Xinguang Yu; Yuanzheng Zhang

    2006-01-01

    BACKGROUND: The wave form, latency and wave amplitude of visual evoked potentials (VEP) are obviously affected by the stimulative parameters, physiological status of the subjects and anesthetics, thus there are greater normal variations and individual differences. The features of flash VEP (F-VEP) are to be observed.OBJECTIVE: To observe and compare the differences of F-VEP latencies and wave amplitudes between eyes in rabbits, and investigate the correlation with sex and the side of eyes.DESIGN: A comparative animal experiment.SETTING: Department of Neurosurgery, General Hospital of Chinese PLA.MATERIALS: The experiment was carried out in the neurophysiological laboratory of the Institute of Neurosciences, General Hospital of Chinese PLA from September 2004 to February 2005. Thirty big-ear rabbits of clean degree, 15 males and 15 females, weighing 2.0-2.5 kg, were provided by the animal center of the General Hospital of Chinese PLA.METHODS: Viking-IV perioperative monitor and flash stimulator for special use were applied. The rabbits were anesthetized with intramuscular injection of compound ketamine. The recording electrode was placed at 3 mm anterior to exoccipital tuberosity (onion, Oz), and the reference electrode was placed at the ear edge of the same side. The stimulative frequency was 1.9 Hz, and the amplifier was 50 μV; The range of wave filter was 5 Hz for high pass and 100 Hz for Iow pass; The average overlapping was 200 times, and the analytical time was 250 ms.MAIN OUTCOME MEASURES: Comparison of F-VEP wave forms; F-VEP latencies and wave amplitudes.RESULTS: All the 30 rabbits were involved in the analysis of results. ① Comparison of F-VEP wave forms in rabbits: The F-VEP waves mainly manifested aspositive-negative-positive (PNP). The F-VEP manifestations to light stimulations were extremely similar between left and right eyes, and the wave amplitudes of both eyes were obviously increased. ② Determinations of F-VEP latencies and wave amplitudes

  9. Selection of reference genes for quantitative real-time PCR normalization in Panax ginseng at different stages of growth and in different organs.

    Science.gov (United States)

    Liu, Jing; Wang, Qun; Sun, Minying; Zhu, Linlin; Yang, Michael; Zhao, Yu

    2014-01-01

    Quantitative real-time reverse transcription PCR (qRT-PCR) has become a widely used method for gene expression analysis; however, its data interpretation largely depends on the stability of reference genes. The transcriptomics of Panax ginseng, one of the most popular and traditional ingredients used in Chinese medicines, is increasingly being studied. Furthermore, it is vital to establish a series of reliable reference genes when qRT-PCR is used to assess the gene expression profile of ginseng. In this study, we screened out candidate reference genes for ginseng using gene expression data generated by a high-throughput sequencing platform. Based on the statistical tests, 20 reference genes (10 traditional housekeeping genes and 10 novel genes) were selected. These genes were tested for the normalization of expression levels in five growth stages and three distinct plant organs of ginseng by qPCR. These genes were subsequently ranked and compared according to the stability of their expressions using geNorm, NormFinder, and BestKeeper computational programs. Although the best reference genes were found to vary across different samples, CYP and EF-1α were the most stable genes amongst all samples. GAPDH/30S RPS20, CYP/60S RPL13 and CYP/QCR were the optimum pair of reference genes in the roots, stems, and leaves. CYP/60S RPL13, CYP/eIF-5A, aTUB/V-ATP, eIF-5A/SAR1, and aTUB/pol IIa were the most stably expressed combinations in each of the five developmental stages. Our study serves as a foundation for developing an accurate method of qRT-PCR and will benefit future studies on gene expression profiles of Panax Ginseng.

  10. The assessment of anthropogenic impact on the environment in East Fennoscandia based on the Normalized Difference Vegetation Index data

    Science.gov (United States)

    Miulgauzen, Daria; Pankratova, Lubov

    2017-04-01

    Being a part of Eurasian "cold sector", ecosystems of East Fennoscandia may fit in the category of the most vulnerable to any external impact, including anthropogenic one. The productivity of plant communities can serve as an indicator representing the state of ecosystems, especially in disturbed areas. The present research is aimed at the environmental impact assessment caused by the Pechenganikel Mining and Metallurgical Plant based on the plant communities' productivity data on the example of ecosystems of East Fennoscandia. Vegetation productivity was assessed on the basis of the Normalized Difference Vegetation Index (NDVI) which is often used for screenings to quantify plant canopy. The essence of the method is that of the difference between the spectral reflectance of vegetation in red and near-infrared regions. The index was calculated on the satellite images of Landsat 8 in IDRISI Kilimanjaro (Clark Labs) according to the equation: N DV I = N-IR- RED-; N IR +RED NIR - spectral reflectance measurements in near-infrared region, RED - spectral reflectance measurements in red region. To compare the index calculations with the information on the state of plant communities, the field studies were carried out in the area of 380 km2 in the vicinity of the Pechenganikel Mining and Metallurgical Plant (Kola Peninsula, Nikel urban-type settlement). As a result, there was created a map in MapInfo Professional 12.5 (Pitney Bowes Software) that represents the vegetation damage at a scale of 1:100,000. The field research has revealed the morphogenetic discrepancy between the soil-plant cover of the area in question and the one of "zonal" ecosystems. Plant communities have been widely modified or destroyed because of air pollution and there are numerous disturbances in the soil profile structure. In terms of vegetation productivity, the analysis of the NDVI figures has shown that the closer the pollution source (Pechenganikel Plant) is, the more significant the

  11. Ultra-structural effects of different low-level lasers on normal cultured human melanocytes: an in vitro comparative study.

    Science.gov (United States)

    AlGhamdi, Khalid M; Kumar, Ashok; A Al-Ghamdi, Attieh; Al-Rikabi, Ammar C; Mubarek, Mohammed; Ashour, Abdelkader E

    2016-12-01

    The aim of this study was to investigate the effects of the different types of low-level laser therapy (LLLT) on the ultra-structure and number of melanosomes in normal cultured human melanocytes. Specific effects of various types of LLLT on the ultra-structure of melanosomes have not yet been reported. Melanocytes were exposed to LLLT at an energy level of 2.0 J/cm(2), using a blue (457 nm), red (635 nm), or ultraviolet (UV) (355 nm) laser. After 72 h of irradiation, the melanocytes were fixed in 2.5 % glutaraldehyde (pH 7.2) phosphate buffer for 8 h and analyzed by transmission electron microscopy. Four developmental stages (I to IV) of melanosomes were observed, and their numbers were counted manually. The percentage of stages I, II, III, and IV melanosomes was 12.8, 14.2, 22.6, and 50.3 %, respectively, in the control (sham light). However, the melanosome percentages were 41.2, 5.4, 8.2, and 24.2 % in stages I, II, III, and IV, respectively, in the blue laser-treated group; 58.4, 6.1, 9.3, and 26.2 % for stages I, II, III, and IV, respectively, in the red laser-treated group; and 31.3, 11.1, 16.5, and 41.1 % for stages I, II, III, and IV, respectively, in the UV laser-treated group. The present data show that the amount of stage I is significantly higher (P < 0.0001) in the LLLT-treated cells compared to the control, which indicates significant stimulation of melanogenesis. The red laser was more effective than the other lasers. Moreover, the effects of LLLT on the ultra-structure of melanosomes need to be studied in a larger number of subject groups.

  12. Trends in the normalized difference vegetation index (NDVI) associated with urban development in arctic and subarctic Western Siberia

    Science.gov (United States)

    Outten, S.; Miles, V.; Ezau, I.

    2015-12-01

    Changes in normalized difference vegetation index (NDVI) in the high Arctic have been reliably documented, with widespread "greening" (increase in NDVI), specifically along the northern rim of Eurasia and Alaska. Whereas in West Siberia south of 65N, widespread "browning" (decrease in NDVI) has been noted, although the causes remain largely unclear. In this study we report results of statistical analysis of the spatial and temporal changes in NDVI around 28 major urban areas in the arctic and subarctic Western Siberia. Exploration and exploitation of oil and gas reserves has led to rapid industrialization and urban development in the region. This development has significant impact on the environment and particularly in the vegetation cover in and around the urbanized areas. The analysis is based on 15 years (2000-2014) of high-resolution (250 m) Moderate Resolution Imaging Spectroradiometer (MODIS) data acquired for summer months (June through August) over the entire arctic and subarctic Western Siberian region. The analysis shows that the NDVI background trends are generally in agreement with the trends reported in previous coarse-resolution NDVI studies. Our study reveals greening over the arctic (tundra and tundra-forest) part of the region. Simultaneously, the southern (boreal taiga forest) part is browning, with the more densely vegetation areas or areas with highest NDVI, particularly along Ob River showing strong negative trend. The unexpected and interesting finding of the study is statistically robust indication of the accelerated increase of NDVI ("greening") in the older urban areas. Many Siberian cities become greener even against the decrease in the NDVI background. Moreover, interannual variations of urban NDVI are not coherent with the NDVI background variability. We also find that in tundra zones, NDVI values are higher in a 5-10 km buffer zone around the city edge than in rural areas (40 km distance from the city edge), and in taiga in a 5-10 km

  13. Same Noses, Different Nasalance Scores: Data from Normal Subjects and Cleft Palate Speakers for Three Systems for Nasalance Analysis

    Science.gov (United States)

    Bressmann, Tim; Klaiman, Paula; Fischbach, Simone

    2006-01-01

    Nasalance scores from the Nasometer, the NasalView and the OroNasal System were compared. The data was collected from 50 normal participants and 19 hypernasal patients with cleft palate. The Nasometer had the lowest nasalance scores for the non-nasal Zoo Passage and that the OroNasal System had the lowest nasalance scores for the Nasal Sentences.…

  14. Effect of Different Surface Treatments on Microtensile Bond Strength of Composite Resin to Normal and Fluorotic Enamel after Microabrasion

    Directory of Open Access Journals (Sweden)

    Mahshid Mohammadi Basir

    2017-01-01

    Full Text Available Objectives: This study aimed to determine the effect of surface treatments such as tooth reduction and extending the etching time on microtensile bond strength (µTBS of composite resin to normal and fluorotic enamel after microabrasion. Materials and Methods: Fifty non-carious anterior teeth were classified into two groups of normal and fluorotic (n=25 using Thylstrup and Fejerskov index (TFI=4-6. Teeth in each group were treated with five modalities as follows and restored with OptiBond FL and Z350 composite resin: 1-Etching (30 seconds, bonding, filling (B; 2-Tooth reduction (0.3mm, etching, bonding, filling (R-B; 3-Microabrasion (120 seconds, etching, bonding, filling (M-B; 4- Microabrasion, tooth reduction, etching, bonding, filling (M-R-B; and 5- Microabrasion, etching (60 seconds, bonding, filling (M-2E-B. Ten experimental groups (n=5 were designed; 150 rectangular samples (10 in each group with a cross-sectional area of 1×1mm2 were prepared for µTBS test. Failure mode was determined under a stereomicroscope and one specimen was selected from each group for scanning electron microscopy (SEM analysis. Data were analyzed using two-way ANOVA and Tukey’s test.Results: The µTBS to normal enamel was higher than to fluorotic enamel in all groups except for group (R-B. The Maximum and minimum µTBS were noted in the group (normal, reduction, bonding and (fluorosed, microabrasion, bonding, respectively.  Tooth reduction increased µTBS more effectively than extended etching time after microabrasion. Conclusions: Fluorosis may reduce µTBS of composite resin to enamel. Microabrasion reduced the bond strength. Tooth reduction and extended etching time increased µTBS of composite resin to both normal and fluorotic enamel.Keywords: Fluorosis, Dental; Enamel Microabrasion; Dental Bonding; Composite Resins

  15. Effect of Different Surface Treatments on Microtensile Bond Strength of Composite Resin to Normal and Fluorotic Enamel After Microabrasion

    Science.gov (United States)

    Bassir, Mahshid Mohammadi; Rezvani, Mohammad Bagher; Hosseini, Zahra Malek

    2016-01-01

    Objectives: This study aimed to determine the effect of surface treatments such as tooth reduction and extending the etching time on microtensile bond strength (μTBS) of composite resin to normal and fluorotic enamel after microabrasion. Materials and Methods: Fifty non-carious anterior teeth were classified into two groups of normal and fluorotic (n=25) using Thylstrup and Fejerskov index (TFI=4–6). Teeth in each group were treated with five modalities as follows and restored with OptiBond FL and Z350 composite resin: 1-Etching (30 seconds), bonding, filling (B); 2-Tooth reduction (0.3mm), etching, bonding, filling (R-B); 3-Microabrasion (120 seconds), etching, bonding, filling (MB); 4- Microabrasion, tooth reduction, etching, bonding, filling (M-R-B); and 5- Microabrasion, etching (60 seconds), bonding, filling (M-2E-B). Ten experimental groups (n=5) were designed; 150 rectangular samples (10 in each group) with a cross-sectional area of 1×1mm2 were prepared for μTBS test. Failure mode was determined under a stereomicroscope and one specimen was selected from each group for scanning electron microscopy (SEM) analysis. Data were analyzed using two-way ANOVA and Tukey’s test. Results: The μTBS to normal enamel was higher than to fluorotic enamel in all groups except for group (R-B). The Maximum and minimum μTBS were noted in the group (normal, reduction, bonding) and (fluorosed, microabrasion, bonding), respectively. Tooth reduction increased μTBS more effectively than extended etching time after microabrasion. Conclusions: Fluorosis may reduce μTBS of composite resin to enamel. Microabrasion reduced the bond strength. Tooth reduction and extended etching time increased μTBS of composite resin to both normal and fluorotic enamel. PMID:28243305

  16. Plantar pressure differences among adults with mild flexible flatfoot, severe flexible flatfoot and normal foot when walking on level surface, walking upstairs and downstairs.

    Science.gov (United States)

    Zhai, Jun Na; Wang, Jue; Qiu, Yu Sheng

    2017-04-01

    [Purpose] This study observed the plantar pressure between flexible flatfoot and normal foot on different walking conditions to find out if flexible flatfoot needs the treatment and how the plantar pressure change while walking upstairs and downstairs. [Subjects and Methods] Fifteen adults with mild flexible flatfoot, fifteen adults with severe flexible flatfoot and fifteen adults with normal foot were examined while walking on a level surface, walking up and down 10 cm and 20 cm stairs. The max force and the arch index were acquired using the RSscan system. The repeated measures ANOVA was performed to analyze the data. [Results] Compared with normal foot, both max force and arch index of severe flatfoot were significantly increased on different walking conditions. When walking down 10 cm and 20 cm stairs, the plantar data of both normal foot and flatfoot were significantly increased. [Conclusion] The plantar pressure of severe flexible flatfoot were significantly larger than that of normal foot on different walking conditions. In addition, the arches of both normal foot and flatfoot were obviously deformed when walking downstairs. It is therefore necessary to be treated for severe flexible flatfoot to prevent further deformation.

  17. Differences in oestrogen receptors in malignant and normal breast tissue as identified by the binding of a new synthetic progestogen.

    OpenAIRE

    Iqbal, M J; Colletta, A. A.; Houmayoun-Valyani, S. D.; Baum, M.

    1986-01-01

    Oestrogen receptor protein (ER) was detected in 9 of 11 samples of malignant breast tissue and 8 of 9 samples of normal breast tissue. Levels of cytosolic ER (ERc) in malignant breast were 21-1102 fmol mg-1 soluble protein (Kd 1.8 X 10(-9)-3.1 X 10(-8) mol l-1) and those of nucleosolic ER (ERn), 13-526 fmol mg-1 soluble protein (Kd 2.1 X 10(-9)-1.4 X 10(-8) mol l-1). In normal breast tissue ERc levels were 33-640 fmol mg-1 soluble protein (Kd 1.3 X 10(-10)-3.2 X 10(-9) mol l-1), ERn was detec...

  18. Identification of Appropriate Reference Genes for Normalization of miRNA Expression in Grafted Watermelon Plants under Different Nutrient Stresses.

    Science.gov (United States)

    Wu, Weifang; Deng, Qin; Shi, Pibiao; Yang, Jinghua; Hu, Zhongyuan; Zhang, Mingfang

    2016-01-01

    Watermelon (Citrullus lanatus) is a globally important crop belonging to the family Cucurbitaceae. The grafting technique is commonly used to improve its tolerance to stress, as well as to enhance its nutrient uptake and utilization. It is believed that miRNA is most likely involved in its nutrient-starvation response as a graft-transportable signal. The quantitative real-time reverse transcriptase polymerase chain reaction is the preferred method for miRNA functional analysis, in which reliable reference genes for normalization are crucial to ensure the accuracy. The purpose of this study was to select appropriate reference genes in scion (watermelon) and rootstocks (squash and bottle gourd) of grafted watermelon plants under normal growth conditions and nutrient stresses (nitrogen and phosphorus starvation). Under nutrient starvation, geNorm identified miR167c and miR167f as two most stable genes in both watermelon leaves and squash roots. miR166b was recommended by both geNorm and NormFinder as the best reference in bottle gourd roots under nutrient limitation. Expression of a new Cucurbitaceae miRNA, miR85, was used to validate the reliability of candidate reference genes under nutrient starvation. Moreover, by comparing several target genes expression in qRT-PCR analysis with those in RNA-seq data, miR166b and miR167c were proved to be the most suitable reference genes to normalize miRNA expression under normal growth condition in scion and rootstock tissues, respectively. This study represents the first comprehensive survey of the stability of miRNA reference genes in Cucurbitaceae and provides valuable information for investigating more accurate miRNA expression involving grafted watermelon plants.

  19. Differences in lifestyle behaviors, dietary habits, and familial factors among normal-weight, overweight, and obese Chinese children and adolescents

    OpenAIRE

    Guo Xiaofan; Zheng Liqiang; Li Yang; Yu Shasha; Sun Guozhe; Yang Hongmei; Zhou Xinghu; Zhang Xingang; Sun Zhaoqing; Sun Yingxian

    2012-01-01

    Abstract Background Pediatric obesity has become a global public health problem. Data on the lifestyle behaviors, dietary habits, and familial factors of overweight and obese children and adolescents are limited. The present study aims to compare health-related factors among normal-weight, overweight, and obese Chinese children and adolescents. Methods We conducted a cross-sectional study consisted of 4262 children and adolescents aged 5–18 years old from rural areas of the northeast China. A...

  20. Identification of Appropriate Reference Genes for Normalization of miRNA Expression in Grafted Watermelon Plants under Different Nutrient Stresses

    Science.gov (United States)

    Wu, Weifang; Deng, Qin; Shi, Pibiao; Yang, Jinghua; Hu, Zhongyuan; Zhang, Mingfang

    2016-01-01

    Watermelon (Citrullus lanatus) is a globally important crop belonging to the family Cucurbitaceae. The grafting technique is commonly used to improve its tolerance to stress, as well as to enhance its nutrient uptake and utilization. It is believed that miRNA is most likely involved in its nutrient-starvation response as a graft-transportable signal. The quantitative real-time reverse transcriptase polymerase chain reaction is the preferred method for miRNA functional analysis, in which reliable reference genes for normalization are crucial to ensure the accuracy. The purpose of this study was to select appropriate reference genes in scion (watermelon) and rootstocks (squash and bottle gourd) of grafted watermelon plants under normal growth conditions and nutrient stresses (nitrogen and phosphorus starvation). Under nutrient starvation, geNorm identified miR167c and miR167f as two most stable genes in both watermelon leaves and squash roots. miR166b was recommended by both geNorm and NormFinder as the best reference in bottle gourd roots under nutrient limitation. Expression of a new Cucurbitaceae miRNA, miR85, was used to validate the reliability of candidate reference genes under nutrient starvation. Moreover, by comparing several target genes expression in qRT-PCR analysis with those in RNA-seq data, miR166b and miR167c were proved to be the most suitable reference genes to normalize miRNA expression under normal growth condition in scion and rootstock tissues, respectively. This study represents the first comprehensive survey of the stability of miRNA reference genes in Cucurbitaceae and provides valuable information for investigating more accurate miRNA expression involving grafted watermelon plants. PMID:27749935

  1. Pilot study on quantitative assessment of muscle imbalance: differences of muscle synergies, equilibrium-point trajectories, and endpoint stiffness in normal and pathological upper-limb movements.

    Science.gov (United States)

    Oku, Takanori; Uno, Kanna; Nishi, Tomoki; Kageyama, Masayuki; Phatiwuttipat, Pipatthana; Koba, Keitaro; Yamashita, Yuto; Murakami, Kenta; Uemura, Mitsunori; Hirai, Hiroaki; Miyazaki, Fumio; Naritomi, Hiroaki

    2014-01-01

    This paper proposes a novel method for assessment of muscle imbalance based on muscle synergy hypothesis and equilibrium point (EP) hypothesis of motor control. We explain in detail the method for extracting muscle synergies under the concept of agonist-antagonist (AA) muscle pairs and for estimating EP trajectories and endpoint stiffness of human upper limbs in a horizontal plane using an electromyogram. The results of applying this method to the reaching movement of one normal subject and one hemiplegic subject suggest that (1) muscle synergies (the balance among coactivation of AA muscle pairs), particularly the synergies that contributes to the angular directional kinematics of EP and the limb stiffness, are quite different between the normal subject and the hemiplegic subject; (2) the concomitant EP trajectory is also different between the normal and hemiplegic subjects, corresponding to the difference of muscle synergies; and (3) the endpoint (hand) stiffness ellipse of the hemiplegic subject becomes more elongated and orientation of the major axis rotates clockwise more than that of the normal subject. The level of motor impairment would be expected to be assessed from a comparison of these differences of muscle synergies, EP trajectories, and endpoint stiffness among normal and pathological subjects using the method.

  2. Left and right reaction time differences to the sound intensity in normal and AD/HD children.

    Science.gov (United States)

    Baghdadi, Golnaz; Towhidkhah, Farzad; Rostami, Reza

    2017-06-01

    Right hemisphere, which is attributed to the sound intensity discrimination, has abnormality in people with attention deficit/hyperactivity disorder (AD/HD). However, it is not studied whether the defect in the right hemisphere has influenced on the intensity sensation of AD/HD subjects or not. In this study, the sensitivity of normal and AD/HD children to the sound intensity was investigated. Nineteen normal and fourteen AD/HD children participated in the study and performed a simple auditory reaction time task. Using the regression analysis, the sensitivity of right and left ears to various sound intensity levels was examined. The statistical results showed that the sensitivity of AD/HD subjects to the intensity was lower than the normal group (p  0.05). However, in control group the left pathway was more sensitive to the sound intensity level than the right one (p = 0.0156). It can be probable that the deficit of the right hemisphere has influenced on the auditory sensitivity of AD/HD children. The possible existent deficits of other auditory system components such as middle ear, inner ear, or involved brain stem nucleuses may also lead to the observed results. The development of new biomarkers based on the sensitivity of the brain hemispheres to the sound intensity has been suggested to estimate the risk of AD/HD. Designing new technique to correct the auditory feedback has been also proposed in behavioral treatment sessions. Copyright © 2017. Published by Elsevier B.V.

  3. Effect of three different calcium hydroxide mixtures (calcium hydroxide with glycerine, normal saline and distilled water on root dentin microhardness

    Directory of Open Access Journals (Sweden)

    Hasheminia SM

    2007-06-01

    Full Text Available Background and Aim: During root canal therapy, it is necessary to remove as many bacteria as possible from the root canal. The use of medicaments is recommended to reduce the microbial population prior to root filling. Calcium hydroxide pastes have been used because of their antibacterial effects and the ability of tissue dissolving. The aim of this study was to evaluate the effect of calcium hydroxide/glycerine mixture, calcium hydroxide/normal saline mixture and calcium hydroxide/distilled water mixture on root dentin microhardness in storage times of 7 and 14 days.Materials and Methods: In this in vitro study, fifteen extracted maxillary canines and central incisors were selected. The crowns of the teeth were removed and the canals were prepared. Teeth were sectioned transversally to produce a total of 30 dentin discs from the middle third of the roots. Specimens were divided into three groups of 10 discs each. Dentin samples were subjected to calcium hydroxide/glycerine, calcium hydroxide/normal saline and calcium hydroxide/distilled water mixtures for 7 and 14 days. Dentin microhardness was measured by a Vickers indenter with a load of 200 g for 15 seconds. Data were analyzed using ANOVA, Paired t-test and LSD with p<0.05 as the level of significance.Results: Statistical analysis showed that all three mixtures decreased dentin microhardness. After 7 days, reduction in dentin microhardness by calcium hydroxide/glycerine combination was significantly higher than calcium hydroxide/normal saline and calcium hydroxide/distilled water combinations. After 14 days, reduction in dentin microhardness by calcium hydroxide/distilled water combination was significantly higher than the other two groups.Conclusion: Based on the results of this study, the use of calcium hydroxide combinations for intracanal dressing reduces dentin microhardness. After 7 days calcium hydroxide/glycerine combination and after 14 days calcium hydroxide/distilled water

  4. [The binding of iron to normal human erythrocyte membranes and its intracellular penetration as a function of different glucides].

    Science.gov (United States)

    Bouvet, D; Boulard, M; Najean, Y

    1975-04-14

    In vivo intestinal absorption of iron in rat is greatly enhanced by Lactose and D-Xylose. Both sugars are also able to increase the amount of iron bound to the red cell membrane in the animal. Similar effects have been noted when using human normal red cells. Lactose of D-Xylose are able to convert into an active transport curve the linear diffusion curve which is noted when iron is used without any ligand. It is possible to quantify the effect of both sugars on the flux of iron towards the red cell membrane.

  5. Interleukin 1 beta induces diabetes and fever in normal rats by nitric oxide via induction of different nitric oxide synthases

    DEFF Research Database (Denmark)

    Reimers, J I; Bjerre, U; Mandrup-Poulsen, T

    1994-01-01

    Substantial in vitro evidence suggests that nitric oxide may be a major mediator of interleukin 1 (IL-1) induced pancreatic beta-cell inhibition and destruction in the initial events leading to insulin-dependent diabetes mellitus. Using NG-nitro-L-arginine methyl ester, an inhibitor of both......, glucagon, corticosterone and leukocyte- and differential-counts in normal rats injected once daily for 5 days with interleukin 1 beta (IL-1 beta) (0.8 microgram/rat = 4.0 micrograms/kg). Inhibition of both the constitutive and the inducible forms of nitric oxide synthase prevented IL-1 beta-induced fever...

  6. Satellite-derived NDVI, LST, and climatic factors driving the distribution and abundance of Anopheles mosquitoes in a former malarious area in northwest Argentina.

    Science.gov (United States)

    Dantur Juri, María Julia; Estallo, Elizabet; Almirón, Walter; Santana, Mirta; Sartor, Paolo; Lamfri, Mario; Zaidenberg, Mario

    2015-06-01

    Distribution and abundance of disease vectors are directly related to climatic conditions and environmental changes. Remote sensing data have been used for monitoring environmental conditions influencing spatial patterns of vector-borne diseases. The aim of this study was to analyze the effect of the Normalized Difference Vegetation Index (NDVI) and Land Surface Temperature (LST) obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS), and climatic factors (temperature, humidity, wind velocity, and accumulated rainfall) on the distribution and abundance of Anopheles species in northwestern Argentina using Poisson regression analyses. Samples were collected from December, 2001 to December, 2005 at three localities, Aguas Blancas, El Oculto and San Ramón de la Nueva Orán. We collected 11,206 adult Anopheles species, with the major abundance observed at El Oculto (59.11%), followed by Aguas Blancas (22.10%) and San Ramón de la Nueva Orán (18.79%). Anopheles pseudopunctipennis was the most abundant species at El Oculto, Anopheles argyritarsis predominated in Aguas Blancas, and Anopheles strodei in San Ramón de la Nueva Orán. Samples were collected throughout the sampling period, with the highest peaks during the spring seasons. LST and mean temperature appear to be the most important variables determining the distribution patterns and major abundance of An. pseudopunctipennis and An. argyritarsis within malarious areas. © 2015 The Society for Vector Ecology.

  7. Transcriptional Differences between Normal and Glioma-Derived Glial Progenitor Cells Identify a Core Set of Dysregulated Genes

    Directory of Open Access Journals (Sweden)

    Romane M. Auvergne

    2013-06-01

    Full Text Available Glial progenitor cells (GPCs are a potential source of malignant gliomas. We used A2B5-based sorting to extract tumorigenic GPCs from human gliomas spanning World Health Organization grades II–IV. Messenger RNA profiling identified a cohort of genes that distinguished A2B5+ glioma tumor progenitor cells (TPCs from A2B5+ GPCs isolated from normal white matter. A core set of genes and pathways was substantially dysregulated in A2B5+ TPCs, which included the transcription factor SIX1 and its principal cofactors, EYA1 and DACH2. Small hairpin RNAi silencing of SIX1 inhibited the expansion of glioma TPCs in vitro and in vivo, suggesting a critical and unrecognized role of the SIX1-EYA1-DACH2 system in glioma genesis or progression. By comparing the expression patterns of glioma TPCs with those of normal GPCs, we have identified a discrete set of pathways by which glial tumorigenesis may be better understood and more specifically targeted.

  8. Differences in lingual vibrotactile threshold shifts during magnitude-estimation scaling between normal-speaking children and children with articulation problems.

    Science.gov (United States)

    Fucci, D; Petrosino, L; Underwood, G; Clark, K

    1992-10-01

    The purpose of this study was to investigate differences in function of the tactile sensory system between groups of normal-speaking children and children with articulation problems. This task was accomplished by studying possible tactile threshold shifts occurring during magnitude-estimation scaling of vibratory stimuli presented to the dorsal surface of the tongue. 10 normal-speaking children (M age = 7.8 yr.) and 9 children with articulation problems (M age = 7.5 yr.) participated. The normal-speaking children and articulatory defective children performed differently on the magnitude-estimation scaling task in which threshold was allowed to return to baseline after each stimulus presentation. These two groups of children also showed dissimilar threshold shifts for the suprathreshold intensities employed in the magnitude-estimation scaling.

  9. Differences in collagen architecture between keloid, hypertrophic scar, normotrophic scar, and normal skin: An objective histopathological analysis

    NARCIS (Netherlands)

    Verhaegen, P.D.; Zuijlen, van P.P.; Pennings, N.M.; Marle, van J.; Niessen, F.B.; Horst, C.; Middelkoop, E.

    2009-01-01

    Normotrophic, hypertrophic, and keloidal scars are different types of scar formation, which all need a different approach in treatment. Therefore, it is important to differentiate between these types of scar, not only clinically but also histopathologically. Differences were explored for collagen or

  10. Joint feedback analysis modeling of nonesterified fatty acids in obese Zucker rats and normal Sprague-Dawley rats after different routes of administration of nicotinic acid.

    Science.gov (United States)

    Tapani, Sofia; Almquist, Joachim; Leander, Jacob; Ahlström, Christine; Peletier, Lambertus A; Jirstrand, Mats; Gabrielsson, Johan

    2014-08-01

    Data were pooled from several studies on nicotinic acid (NiAc) intervention of fatty acid turnover in normal Sprague-Dawley and obese Zucker rats in order to perform a joint PKPD of data from more than 100 normal Sprague-Dawley and obese Zucker rats, exposed to several administration routes and rates. To describe the difference in pharmacodynamic parameters between obese and normal rats, we modified a previously published nonlinear mixed effects model describing tolerance and oscillatory rebound effects of NiAc on nonesterified fatty acids plasma concentrations. An important conclusion is that planning of experiments and dose scheduling cannot rely on pilot studies on normal animals alone. The obese rats have a less-pronounced concentration-response relationship and need higher doses to exhibit desired response. The relative level of fatty acid rebound after cessation of NiAc administration was also quantified in the two rat populations. Building joint normal-disease models with scaling parameter(s) to characterize the "degree of disease" can be a useful tool when designing informative experiments on diseased animals, particularly in the preclinical screen. Data were analyzed using nonlinear mixed effects modeling, for the optimization, we used an improved method for calculating the gradient than the usually adopted finite difference approximation. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

  11. Joint Feedback Analysis Modeling of Nonesterified Fatty Acids in Obese Zucker Rats and Normal Sprague–Dawley Rats after Different Routes of Administration of Nicotinic Acid

    Science.gov (United States)

    Tapani, Sofia; Almquist, Joachim; Leander, Jacob; Ahlström, Christine; Peletier, Lambertus A; Jirstrand, Mats; Gabrielsson, Johan

    2014-01-01

    Data were pooled from several studies on nicotinic acid (NiAc) intervention of fatty acid turnover in normal Sprague–Dawley and obese Zucker rats in order to perform a joint PKPD of data from more than 100 normal Sprague–Dawley and obese Zucker rats, exposed to several administration routes and rates. To describe the difference in pharmacodynamic parameters between obese and normal rats, we modified a previously published nonlinear mixed effects model describing tolerance and oscillatory rebound effects of NiAc on nonesterified fatty acids plasma concentrations. An important conclusion is that planning of experiments and dose scheduling cannot rely on pilot studies on normal animals alone. The obese rats have a less-pronounced concentration–response relationship and need higher doses to exhibit desired response. The relative level of fatty acid rebound after cessation of NiAc administration was also quantified in the two rat populations. Building joint normal-disease models with scaling parameter(s) to characterize the “degree of disease” can be a useful tool when designing informative experiments on diseased animals, particularly in the preclinical screen. Data were analyzed using nonlinear mixed effects modeling, for the optimization, we used an improved method for calculating the gradient than the usually adopted finite difference approximation. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:2571–2584, 2014 PMID:24986056

  12. Gender differences in nigrostriatal dopaminergic innervation are present at young-to-middle but not at older age in normal adults.

    NARCIS (Netherlands)

    Wong, K.K.; Muller, M.L.; Kuwabara, H.; Studenski, S.A.; Bohnen, N.I.

    2012-01-01

    Gender differences in brain dopaminergic activity have been variably reported in the literature. We performed an evaluation for gender effects on striatal dopamine transporter (DAT) binding in a group of normal subjects. Community-dwelling adults (n = 85, 50F/35M, mean age 62.7 +/- 16.2 SD, range 20

  13. Coronal View Ultrasound Imaging of Movement in Different Segments of the Tongue during Paced Recital: Findings from Four Normal Speakers and a Speaker with Partial Glossectomy

    Science.gov (United States)

    Bressmann, Tim; Flowers, Heather; Wong, Willy; Irish, Jonathan C.

    2010-01-01

    The goal of this study was to quantitatively describe aspects of coronal tongue movement in different anatomical regions of the tongue. Four normal speakers and a speaker with partial glossectomy read four repetitions of a metronome-paced poem. Their tongue movement was recorded in four coronal planes using two-dimensional B-mode ultrasound…

  14. The Effect of Spinal Tap Test on Different Sensory Modalities of Postural Stability in Idiopathic Normal Pressure Hydrocephalus

    Directory of Open Access Journals (Sweden)

    Katrin Abram

    2016-09-01

    Full Text Available Background/Aims: Postural instability in patients with normal pressure hydrocephalus (NPH is a most crucial symptom leading to falls with secondary complications. The aim of the current study was to evaluate the therapeutic effect of spinal tap on postural stability in these patients. Methods: Seventeen patients with clinical symptoms of NPH were examined using gait scale, computerized dynamic posturography (CDP, and neuropsychological assessment. Examinations were done before and after spinal tap test. Results: The gait score showed a significant improvement 24 h after spinal tap test in all subtests and in the sum score (p Conclusion: Postural stability in NPH is predominantly affected by deficient vestibular functions, which did not improve after spinal tap test. Conditions which improved best were mainly independent from visual control and are based on proprioceptive functions.

  15. [Composition and metabolism of extremely low-density lipoproteins in normal rabbits of different age and under experimental atherosclerosis].

    Science.gov (United States)

    Chaialo, P P

    1981-01-01

    The lipid composition and protein content in extremely low-density lipoproteids. lipoproteid-lypase activity and breakdown dynamics of the mentioned [3H]cholesterol-labelled lipoproteids are studied in blood of young and old rabbits in norm and under experimental atherosclerosis. It is shown that in normal animals the content of all lipid components and protein decreases with ageing in the fraction of extremely low-density lipoproteids isolated from blood serum. The activity of lipoproteid lypase and breakdown of intravenously injected labelled extremely low-density lipoproteids are lowered in old animals as well. Under experimental atherosclerosis in extremely low-density lipoproteids there occurs accumulation of cholesterol esters in considerable amounts, the breakdown of the class lipoproteids is slown down. The enzymic activity manifests no significant changes in comparison with the norm. Disturbances in the composition of lipoproteids and their metabolism with the studied pathology are more pronounced in old rabbits than in young ones.

  16. [Different significance in normal subjects and in glaucoma patients tested with Optopol PTS-910, in the galucoma program].

    Science.gov (United States)

    Dascalu, Ana Maria; Cherecheanu, Alina Popa; Stana, Daniela; Serban, Dragoş

    2013-01-01

    to quantify the inter-test variability (dB) for the Optopol PTS automated perimeter, Glaucoma Fast threshold program. A prospective study was performed on 166 glaucomatous patients and a control group of 30 normal subjects, tested by complete ophthalmological exam and automated perimetry (Optopol PTS-910). The visual field was tested weekly for 4 consecutive weeks. The visual field defects were classified according to the Aulhorn-Karmeyer descriptive scale. For the control group, the medium inter-test variability was of 1.57 +/- 0.24 dB, lower next to fixation and increasing towards the 50 degree isopter. The medium inter-test variability increases along with the perimetric stage :1.57 +/- 0.66 dB for pre-perimetric glaucoma, 2.13 + 1.04 dB for non-specific defects group, 3.23 + 1.01 dB for the stage 1, 3.52 + 2.61 dB, for the stage 2, 3.65 + 1.19dB for the stage 3 and 5.82 +/- 1.67dB for the stage 4. For the cases of preperimetric glaucoma and non-specific defects, a similar profile of variability to the normal subjects can be observed. For the stages 2-4, the profile of the areas with maxim inter-test variability moves towards the relative scotoma and the surrounding area. A better description of the inter-test variability and the evolution of this intricate parameter of the retinal light sensitivity is useful for the differential diagnostic between the real change and the "background noise" in early detection of the functional progression in glaucoma.

  17. Insomnia in depression: differences in objective and subjective sleep and awakening quality to normal controls and acute effects of trazodone.

    Science.gov (United States)

    Saletu-Zyhlarz, Gerda Maria; Abu-Bakr, Manal Hassan; Anderer, Peter; Gruber, Georg; Mandl, Magdalena; Strobl, Roland; Gollner, Dietmar; Prause, Wolfgang; Saletu, Bernd

    2002-02-01

    Utilizing polysomnography (PSG) and psychometry, objective and subjective sleep and awakening quality was investigated in 11 drug-free patients (five females, six males) aged 35-75 years (mean age 54.1 +/- 11.4) suffering from nonorganic insomnia (F 51.0) related to a depressive episode (F 32) or recurrent depressive disorder (F 33). as compared with 11 age- and sex-matched normal controls (five females, six males) aged 36-75 years (mean age 53.0 +/- 13.5). PSG demonstrated decreased sleep efficiency, total sleep time (TST), total sleep period (TSP) and sleep stage S2, as well as increased wakefulness during TSP, early morning awakening, sleep latency to S1, S2, S3 and sleep stage S1 in depressed patients. Subjective sleep quality and the total score of the Self-Assessment of Sleep and Awakening Quality Scale (SSA) were deteriorated as were morning and evening well being, drive, mood and fine motor activity right. Evening and morning blood pressure, the O2 desaturation index and periodic leg movement (PLM) index were increased. In a subsequent acute, placebo-controlled cross-over design study, the acute effects of 100 mg of trazodone, a serotonin reuptake inhibitor with a sedative action due to 5-HT2 and alpha1 receptor blockade, were investigated in the patients. As compared with placebo, trazodone induced an increase in sleep efficiency (primary target variable), TST, TSP and SWS (S3 + S4), as well as a decrease in wakefulness during the TSP, early morning awakening and S2. There was no change in rapid eye movement (REM) sleep with the exception of an increase in the REM duration in minutes. Trazodone also caused an improvement in subjective sleep quality, affectivity, numerical memory and somatic complaints. All respiratory variables remained within normal limits. Critical flicker frequency and moming diastolic blood pressure were decreased. The present study demonstrated that depression induced significant changes in objective and subjective sleep and awakening

  18. Similarities and differences between eating disorders and obese patients in a virtual environment for normalizing eating patterns.

    Science.gov (United States)

    Perpiñá, Conxa; Roncero, María

    2016-05-01

    Virtual reality has demonstrated promising results in the treatment of eating disorders (ED); however, few studies have examined its usefulness in treating obesity. The aim of this study was to compare ED and obese patients on their reality judgment of a virtual environment (VE) designed to normalize their eating pattern. A second objective was to study which variables predicted the reality of the experience of eating a virtual forbidden-fattening food. ED patients, obese patients, and a non-clinical group (N=62) experienced a non-immersive VE, and then completed reality judgment and presence measures. All participants rated the VE with similar scores for quality, interaction, engagement, and ecological validity; however, ED patients obtained the highest scores on emotional involvement, attention, reality judgment/presence, and negative effects. The obese group gave the lowest scores to reality judgment/presence, satisfaction and sense of physical space, and they held an intermediate position in the attribution of reality to virtually eating a "fattening" food. The palatability of a virtual food was predicted by attention capturing and belonging to the obese group, while the attribution of reality to the virtual eating was predicted by engagement and belonging to the ED group. This study offers preliminary results about the differential impact on ED and obese patients of the exposure to virtual food, and about the need to implement a VE that can be useful as a virtual lab for studying eating behavior and treating obesity.

  19. Serum Alanine Aminotransferase Levels within Normal Range Have Different Associations with Augmentation Index and Other Cardiometabolic Risk Factors in Nondrinkers and Drinkers: A Chinese Community-Based Analysis

    Directory of Open Access Journals (Sweden)

    Shihui Fu

    2017-01-01

    Full Text Available Background. To investigate whether serum alanine aminotransferase (ALT levels within normal range were associated with augmentation index (AIx and cardiometabolic risk factors in nondrinkers and drinkers in Chinese community-dwelling population. Methods. There were 4165 participants with serum ALT levels within normal range. Results. Alcohol drinking was observed in 1173 participants (28.2%. In multivariate analysis, serum ALT levels of nondrinkers were independently associated with age, sex, body mass index (BMI, hypertension, diabetes mellitus, diastolic blood pressure, triglyceride, low-density lipoprotein-cholesterol (LDL-c, and AIx, while serum ALT levels of drinkers were independently associated with age, sex, BMI, triglyceride, and LDL-c (p<0.05 for all. Conclusions. Associations of serum ALT levels within normal range with age, sex, body height and weight, and blood lipid were simultaneously present in participants with and without alcohol drinking, while associations of serum ALT levels within normal range with AIx, blood pressure, and glucose were seen in nondrinkers rather than in drinkers. These findings not only provide the evidence that serum ALT levels, even within the normal range, have different associations with arteriosclerosis and cardiometabolic risk factors in nondrinkers and drinkers but also are helpful in understanding the underlying pathophysiologic mechanisms linking the hepatic function to arteriosclerosis and cardiometabolic risk factors.

  20. Difference between Toxicities of Iron Oxide Magnetic Nanoparticles with Various Surface-Functional Groups against Human Normal Fibroblasts and Fibrosarcoma Cells

    Directory of Open Access Journals (Sweden)

    Jaewook Lee

    2013-10-01

    Full Text Available Recently, many nanomedical studies have been focused on magnetic nanoparticles (MNPs because MNPs possess attractive properties for potential uses in imaging, drug delivery, and theranostics. MNPs must have optimized size as well as functionalized surface for such applications. However, careful cytotoxicity and genotoxicity assessments to ensure the biocompatibility and biosafety of MNPs are essential. In this study, Fe3O4 MNPs of different sizes (approximately 10 and 100–150 nm were prepared with different functional groups, hydroxyl (–OH and amine (–NH2 groups, by coating their surfaces with tetraethyl orthosilicate (TEOS, 3-aminopropyltrimethoxysilane (APTMS or TEOS/APTMS. Differential cellular responses to those surface-functionalized MNPs were investigated in normal fibroblasts vs. fibrosarcoma cells. Following the characterization of MNP properties according to size, surface charge and functional groups, cellular responses to MNPs in normal fibroblasts and fibrosarcoma cells were determined by quantifying metabolic activity, membrane integrity, and DNA stability. While all MNPs induced just about 5% or less cytotoxicity and genotoxicity in fibrosarcoma cells at lower than 500 μg/mL, APTMS-coated MNPs resulted in greater than 10% toxicity against normal cells. Particularly, the genotoxicity of MNPs was dependent on their dose, size and surface charge, showing that positively charged (APTMS- or TEOS/APTMS-coated MNPs induced appreciable DNA aberrations irrespective of cell type. Resultantly, smaller and positively charged (APTMS-coated MNPs led to more severe toxicity in normal cells than their cancer counterparts. Although it was difficult to fully differentiate cellular responses to various MNPs between normal fibroblasts and their cancer counterparts, normal cells were shown to be more vulnerable to internalized MNPs than cancer cells. Our results suggest that functional groups and sizes of MNPs are critical determinants of degrees

  1. An assessment of racial differences in the upper limits of normal ALT levels in children and the effect of obesity on elevated values.

    Science.gov (United States)

    Kliethermes, S; Ma, M; Purtell, C; Balasubramanian, N; Gonzalez, B; Layden, T J; Cotler, S J

    2017-10-01

    Childhood obesity is a risk factor for non-alcoholic fatty liver disease and poses important public health issues for children. Racial differences in alanine aminotransferase (ALT) levels among children have not been described. This study aimed to identify racial differences in upper limit normal (ULN) ALT levels and evaluate the effect of obesity on elevated levels in children without other metabolic risk factors. National Health and Nutrition Examination Surveys and clinical data from the Loyola University Health System were used to determine ULN ALT by race and gender. Quantile regression was used to evaluate the impact of obesity on elevated ALT and to identify potential risk factors for ALT above the ULN. Upper limit normal (ULN) ALT was approximately 28.0 and 21.0-24.0 U/L for boys and girls, respectively. No significant difference in ULN ALT across race was observed. Obesity was significantly associated with elevated ALT; obese children with elevated ALT had values 10 U/L higher than normal-weight children. Racial differences in ALT levels among adults are not evident in children. Obesity, in the absence of metabolic risk factors and other causes of liver disease, is associated with elevated ALT, providing evidence against the concept of healthy obesity in children. © 2016 World Obesity Federation.

  2. Three different profiles: early socio-communicative capacities in typical Rett syndrome, the preserved speech variant and normal development.

    Science.gov (United States)

    Marschik, Peter B; Bartl-Pokorny, Katrin D; Tager-Flusberg, Helen; Kaufmann, Walter E; Pokorny, Florian; Grossmann, Tobias; Windpassinger, Christian; Petek, Erwin; Einspieler, Christa

    2014-02-01

    This is the first study aiming to compare pre-diagnostic socio-communicative development of a female with typical Rett syndrome (RTT), a female with the preserved speech variant of RTT (PSV) and a control toddler. We analysed 1275 min of family videos at the participants' age between 9 and 24 months and used the Inventory of Potential Communicative Acts (IPCA) to delineate their repertoires of communicative forms and functions. The results revealed different profiles for the three different conditions. The repertoire of communicative gestures and (pre)linguistic vocalizations was most comprehensive in the control toddler, followed by the female with PSV and the female with RTT. These findings contribute to the growing knowledge about early developmental abnormalities in RTT. In order to define distinctive profiles for typical and atypical RTT and evaluate their specificity, a larger body of evidence is needed.

  3. Canine epidermal lipid sampling by skin scrub revealed variations between different body sites and normal and atopic dogs

    OpenAIRE

    Angelbeck-Schulze, Mandy; Mischke, Reinhard; Rohn, Karl; Hewicker-Trautwein, Marion; Naim, Hassan Y.; Bäumer, Wolfgang

    2014-01-01

    Background Previously, we evaluated a minimally invasive epidermal lipid sampling method called skin scrub, which achieved reproducible and comparable results to skin scraping. The present study aimed at investigating regional variations in canine epidermal lipid composition using the skin scrub technique and its suitability for collecting skin lipids in dogs suffering from certain skin diseases. Eight different body sites (5 highly and 3 lowly predisposed for atopic lesions) were sampled by ...

  4. Canine epidermal lipid sampling by skin scrub revealed variations between different body sites and normal and atopic dogs

    OpenAIRE

    Angelbeck-Schulze, Mandy; Mischke, Reinhard; Rohn, Karl; Hewicker-Trautwein, Marion; Naim, Hassan Y.; Bäumer, Wolfgang

    2014-01-01

    Background Previously, we evaluated a minimally invasive epidermal lipid sampling method called skin scrub, which achieved reproducible and comparable results to skin scraping. The present study aimed at investigating regional variations in canine epidermal lipid composition using the skin scrub technique and its suitability for collecting skin lipids in dogs suffering from certain skin diseases. Eight different body sites (5 highly and 3 lowly predisposed for atopic lesions) were sampled by ...

  5. A study of fluctuating asymmetry in hybrids of dwarf and normal lake whitefish ecotypes (Coregonus clupeaformis) from different glacial races.

    Science.gov (United States)

    Lu, G; Bernatchez, L

    1999-12-01

    Fish ecotypes found in north temperate lakes are increasingly used as model organisms to explore patterns and processes of population divergence that may ultimately cause speciation. Processes involved in their reproductive isolation are, however, still poorly understood. Recent experimental studies on whitefish ecotypes from different glacial races revealed that embryonic mortality of hybrids was 2.4-4.7 times higher than for parental forms. In this study, we compared fluctuating asymmetry (FA) in morphological traits of these same hybrid and pure crosses to test the hypothesis that genetic stress observed in hybrids at embryonic stages is also manifested at later developmental stages. Twelve morphological traits were used to measure asymmetry. Variable degrees of asymmetry were observed depending on traits and crosses, however there was no significant difference in FA among crosses. These results thus provided no evidence in support of the working hypothesis and indicated that genetic stress may differ among life stages. It is more likely that high hybrid embryonic mortality acts together with ecological factors at later stages to increase further the extent of reproductive isolation between sympatric whitefish ecotypes.

  6. Satellite-derived primary productivity and its spatial and temporal variability in the China seas%中国近海初级生产力的遥感研究及其时空演化

    Institute of Scientific and Technical Information of China (English)

    檀赛春; 石广玉

    2006-01-01

    The spatial and temporal variability of primary productivity in the China seas from 2003 to 2005 was estimated using a size-fractionated primary productivity model. Primary productivity estimated from satellite-derived data showed spatial and temporal variability. Annual averaged primary productivity levels were 564.39, 363.08, 536.47, 413.88, 195.77, and 100.09 gCm-2a-1 in the Bohai Sea, northern Yellow Sea (YS), southern YS, northern East China Sea (ECS), southern ECS, and South China Sea (SCS), respectively. Peaks of primary productivity appeared in spring (April-June) and fall (October and November) in the northern YS, southern YS, and southern ECS, while a single peak (June) appeared in the Bohai Sea and northern ECS. The SCS had two peaks in primary productivity, but these peaks occurred in winter (January) and summer (August), with the winter peak far higher than the summer peak. Monthly averaged primary productivity values from 2003 to 2005 in the Bohai Sea and southern YS were higher than those in the other four seas during most months, while those in the southern ECS and SCS were the lowest. Primary productivity in spring (March-June in the southern ECS and April-July in the other five areas) contributed approximately 41% on average to the annual primary productivity in all the study seas except the SCS. The largest interannual variability also occurred in spring (average standard deviation = 6.68), according to the satellite-derived estimates. The contribution during fall (October-January in the southern ECS and August-November in the other five areas) was approximately 33% on average; the primary productivity during this period also showed interannual variability. However, in the SCS, the winter (December-March) contribution was the highest (about 42%), while the spring (April-July) contribution was the lowest (28%). The SCS did share a feature with the other five areas: the larger the contribution, the larger the interannual variability. Spatial and

  7. Gastric potential difference and pH in ulcer patients and normal volunteers during Stroop's colour word conflict test

    DEFF Research Database (Denmark)

    Højgaard, L; Bendtsen, Flemming

    1989-01-01

    . The volunteers fell into two groups: In seven no sympathetic activation was elicited as no changes in heart rate were demonstrated. Gastric pH was unchanged, and PD increased slightly. Sympathetic activation was elicited in the other six with increased heart rate by 18 (6) beats per min. Potential difference...... declined significantly during sympathetic activation (delta PD = -5 (2)mV, p less than 0.05). Gastric pH increased. Eleven of 12 ulcer patients had sympathetic activation accompanied by a decline in PD, and an increased pH. Sympathetic activation in ulcer patients and volunteers impaired gastric mucosal...

  8. Gastric potential difference and pH in ulcer patients and normal volunteers during Stroop's colour word conflict test

    DEFF Research Database (Denmark)

    Højgaard, L; Bendtsen, Flemming

    1989-01-01

    Whether mental stress is important in the pathogenesis of gastric mucosal disorders is not clearly established. This study investigated the relationship between sympathetic activation caused by the Stroop's colour word conflict test and gastric mucosal function, monitored by measuring the gastric...... mucosal electrical potential difference (PD). In 13 healthy volunteers and 12 duodenal ulcer patients gastric PD, pH, and heart rate were measured continuously during basal conditions, during mental stress evoked by the Stroop's colour word conflict test, and after return to basal conditions...

  9. Cerebrospinal Fluid Amyloid-β 42, Total Tau and Phosphorylated Tau are Low in Patients with Normal Pressure Hydrocephalus: Analogies and Differences with Alzheimer's Disease.

    Science.gov (United States)

    Santangelo, Roberto; Cecchetti, Giordano; Bernasconi, Maria Paola; Cardamone, Rosalinda; Barbieri, Alessandra; Pinto, Patrizia; Passerini, Gabriella; Scomazzoni, Francesco; Comi, Giancarlo; Magnani, Giuseppe

    2017-01-01

    Co-existence of Alzheimer's disease (AD) in normal pressure hydrocephalus (NPH) is a frequent finding, thus a common pathophysiological basis between AD and NPH has been postulated. We measured CSF amyloid-β 42 (Aβ42), total tau (t-tau), and phosphorylated tau (p-tau) concentrations in a sample of 294 patients with different types of dementia and 32 subjects without dementia. We then compared scores on neuropsychological tests of NPH patients with pathological and normal CSF Aβ42 values. Aβ42 levels were significantly lower in NPH than in control patients, with no significant differences between AD and NPH. On the contrary, t-tau and p-tau levels were significantly lower in NPH than in AD, with no differences between NPH and controls. NPH patients with pathological Aβ42 levels did not perform worse than NPH patients with normal Aβ42 levels in any cognitive domains. Our data seem to support the hypothesis of amyloid accumulation in brains of NPH patients. Nevertheless, amyloid does not seem to play a pathogenetic role in the development of cognitive deficits in NPH.

  10. Gastric potential difference and pH in ulcer patients and normal volunteers during Stroop's colour word conflict test

    DEFF Research Database (Denmark)

    Højgaard, L; Bendtsen, Flemming

    1989-01-01

    mucosal electrical potential difference (PD). In 13 healthy volunteers and 12 duodenal ulcer patients gastric PD, pH, and heart rate were measured continuously during basal conditions, during mental stress evoked by the Stroop's colour word conflict test, and after return to basal conditions...... declined significantly during sympathetic activation (delta PD = -5 (2)mV, p less than 0.05). Gastric pH increased. Eleven of 12 ulcer patients had sympathetic activation accompanied by a decline in PD, and an increased pH. Sympathetic activation in ulcer patients and volunteers impaired gastric mucosal......Whether mental stress is important in the pathogenesis of gastric mucosal disorders is not clearly established. This study investigated the relationship between sympathetic activation caused by the Stroop's colour word conflict test and gastric mucosal function, monitored by measuring the gastric...

  11. Different sensitivities to rocuronium of the neuromuscular junctions innervated by normal/damaged facial nerves and somatic nerve in rats: the role of the presynaptic acetylcholine quantal release

    Institute of Scientific and Technical Information of China (English)

    CHEN Jun-liang; LI Shao-qin; CHI Fang-lu; CHEN Lian-hua; LI Shi-tong

    2012-01-01

    Background Muscles present different responses to muscle relaxants,a mechanism of importance in surgeries requiring facial nerve evoked electromyography under general anaesthesia.The non-depolarizing muscle relaxants have multiple reaction formats in the neuromuscular junction,in which pre-synaptic quantal release of acetylcholine was one of the important mechanisms.This study was to compare the pre-synaptic quantal release of acetylcholine from the neuromuscular junctions innervated by normal/damaged facial nerves and somatic nerve under the effect of rocuronium in rats in vitro.Methods Acute right-sided facial nerve injury was induced by nerve crush axotomies.Both sided facial nerve connected orbicularis oris strips and tibial nerve connected gastrocnemius strips were isolated to measure endplate potentials (EPP) and miniature endplate potentials (MEPP) using an intracellular microelectrode gauge under different rocuronium concentrations.Then,the pre-synaptic quantal releases of acetylcholine were calculated by the ratios of the EPPs and the MEPPs,and compared among the damaged or normal facial nerve innervated orbicularis oris and tibial nerve innervated gastrocnemius.Results The EPP/MEPP ratios of the three neuromuscular junctions decreased in a dose dependent manner with the increase of the rocuronium concentration.With the concentrations of rocuronium being 5 μg/ml,7.5 μg/ml and 10 μg/ml,the decrease of the EPP/MEPP ratio in the damaged facial nerve group was greater than that in the normal facial nerve group.The decrease in the somatic nerve group was the biggest,with significant differences.Conclusions Rocuronium presented different levels of inhibition on the pre-synaptic quantal release of acetylcholine in the three groups of neuromuscular junctions.The levels of the inhibition showed the following sequence:somatic nerve >damaged facial nerve > normal facial nerve.The difference may be one of the reasons causing the different sensitivities to

  12. Human Renal Normal, Tumoral, and Cancer Stem Cells Express Membrane-Bound Interleukin-15 Isoforms Displaying Different Functions

    Directory of Open Access Journals (Sweden)

    Sandy Azzi

    2015-06-01

    Full Text Available Intrarenal interleukin-15 (IL-15 participates to renal pathophysiology, but the role of its different membrane-bound isoforms remains to be elucidated. In this study, we reassess the biology of membrane-bound IL-15 (mb-IL-15 isoforms by comparing primary cultures of human renal proximal tubular epithelial cells (RPTEC to peritumoral (ptumTEC, tumoral (RCC, and cancer stem cells (CSC/CD105+. RPTEC express a 14 to 16 kDa mb-IL-15, whose existence has been assumed but never formally demonstrated and likely represents the isoform anchored at the cell membrane through the IL-15 receptor α (IL-15Rα chain, because it is sensitive to acidic treatment and is not competent to deliver a reverse signal. By contrast, ptumTEC, RCC, and CSC express a novel N-hyperglycosylated, short-lived transmembrane mb-IL-15 (tmb-IL-15 isoform around 27 kDa, resistant to acidic shock, delivering a reverse signal in response to its soluble receptor (sIL-15Rα. This reverse signal triggers the down-regulation of the tumor suppressor gene E-cadherin in ptumTEC and RCC but not in CSC/CD105+, where it promotes survival. Indeed, through the AKT pathway, tmb-IL-15 protects CSC/CD105+ from non-programmed cell death induced by serum starvation. Finally, both mb-IL-15 and tmb-IL-15 are sensitive to metalloproteases, and the cleaved tmb-IL-15 (25 kDa displays a powerful anti-apoptotic effect on human hematopoietic cells. Overall, our data indicate that both mb-IL-15 and tmb-IL-15 isoforms play a complex role in renal pathophysiology downregulating E-cadherin and favoring cell survival. Moreover, “apparently normal” ptumTEC cells, sharing different properties with RCC, could contribute to organize an enlarged peritumoral “preneoplastic” environment committed to favor tumor progression.

  13. Impact of hemolysis during sample collection: how different is drug concentration in hemolyzed plasma from that of normal plasma?

    Science.gov (United States)

    Tan, Aimin; Gagné, Sébastien; Lévesque, Isabelle A; Lachance, Sylvain; Boudreau, Nadine; Lévesque, Ann

    2012-07-15

    Hemolysis is a common phenomenon in clinical studies. Despite the growing interest in hemolysis matrix effect, how hemolysis impacts the representability of hemolyzed plasma samples was rarely evaluated. The purpose of this research is to perform such an evaluation by theoretical consideration and experiment. A formula for estimating the impact is proposed, which includes the degree of hemolysis and the drug's red blood cell (RBC): plasma concentration ratio. The impact of hemolysis on the representability of hemolyzed plasma samples is compound-dependant. Given the same degree of hemolysis, the stronger a drug binds to RBCs, the more significant the impact of hemolysis. For a drug with high affinity to RBCs, the results of hemolyzed plasma samples may not be useful even though they are accurate. There is an overall agreement between theoretical predication and experimental results. Among the ten different drug compounds tested, only methazolamide, which binds strongly to RBCs, showed significant change in plasma concentration due to hemolysis. Copyright © 2012 Elsevier B.V. All rights reserved.

  14. Differences in tumour and normal tissue concentrations of iodine- and indium-labelled monoclonal antibody. Pt. 2

    Energy Technology Data Exchange (ETDEWEB)

    Pimm, M.V.; Baldwin, R.W.; Perkins, A.C.

    1985-12-01

    The blood, tumour and whole-body levels and survivals of /sup 131/I- and /sup 111/In-labelled monoclonal antibody (791T/36) have been compared in mice with human tumour xenografts. The blood levels and survivals of /sup 131/I- and /sup 111/In-labelled antibody were similar when expressed as proportions of the injected doses. However, the whole-body survival of /sup 111/In following administration of /sup 111/In-labelled antibody was over twice as long as that of /sup 131/I after administration of /sup 131/I-labelled antibody, principally because free /sup 131/I was rapidly excreted but free /sup 111/In was retained, primarily in liver, spleen and kidneys. Consequently, when expressed in relation to the whole body, blood levels of /sup 111/In became progressively lower than those of /sup 131/I following administration of labelled antibodies. In mice with human tumour xenografts the proportion of the injected dose of /sup 111/In from /sup 111/In-labelled antibody deposited in tumour tissue was 4-5 times higher than that of /sup 131/I from /sup 131/I-labelled antibody. When compared with the whole-body levels of radiolabel the difference was less marked, although /sup 111/In accumulation in tumour was more rapid. The higher levels and longer retention of /sup 111/In in tumour produced tumour-to-blood ratios that were 7-8 times those achieved with /sup 131/I-labelled antibody.

  15. Age-related changes in BDNF protein levels in human serum: differences between autism cases and normal controls.

    Science.gov (United States)

    Katoh-Semba, Ritsuko; Wakako, Rie; Komori, Taku; Shigemi, Hiroko; Miyazaki, Noriko; Ito, Hironori; Kumagai, Toshiyuki; Tsuzuki, Masako; Shigemi, Kenji; Yoshida, Futoshi; Nakayama, Atsuo

    2007-10-01

    Accumulating evidence suggests the possible association between the concentrations of serum brain-derived neurotrophic factor (BDNF) and psychiatric disease with impaired brain development. Yet the reasons remain unclear. We therefore investigated the characteristics of serum BDNF as well as its age-related changes in healthy controls in comparison to autism cases. BDNF was gradually released from platelets at 4 degrees C, reached a maximal concentration after around 24 h, and remained stable until 42 h. At room temperature, BDNF was found to be immediately degraded. Circadian changes, but not seasonal changes, were found in serum levels of BDNF existing as the mature form with a molecular mass of 14 kDa. In healthy controls, the serum BDNF concentration increased over the first several years, then slightly decreased after reaching the adult level. There were no sex differences between males and females. In the autism cases, mean levels were significantly lower in children 0-9 years old compared to teenagers or adults, or to age-matched healthy controls, indicating a delayed BDNF increase with development. In a separate study of adult rats, a circadian change in serum BDNF was found to be similar to that in the cortex, indicating a possible association with cortical functions.

  16. Tribological evaluation of refined, bleached and deodorized palm stearin using four-ball tribotester with different normal loads

    Institute of Scientific and Technical Information of China (English)

    Chiong Ing TIONG; Yahya AZLI; Mohammed Rafiq ABDUL KADIR; Samion SYAHRULLAIL

    2012-01-01

    The effects of the mechanical factors with applied loads on the tribological performance of refined,bleached and deodorised (RBD) palm stearin (PS) were studied using a four-ball tribotester.All the RBD PS results were simultaneously compared with the additive-free paraffinic mineral oil (PMO).The experiments were carried out using different loads with a constant speed in order to gain a better understanding of the mechanical processes that occurred during the experiment.For each experiment,the temperature was increased to 75℃ and was run for 1 h.In a mechanical system,lubricant plays an important role in reducing wear and friction.PS exists as a semi-solid at room temperature after the fractionation process from oil palm.Due to the increasing rate of pollution to the environment,vegetable oil was chosen as the test lubricant with regard to its biodegradability.Other advantages of vegetable oil are that it is more easily harvestable and non-toxic compared to petroleum-based oil,which made it a suitable candidate.From the experiment,RBD PS is found to have a better friction constraint reduction compared with additive-free PMO.

  17. Clarifying Normalization

    Science.gov (United States)

    Carpenter, Donald A.

    2008-01-01

    Confusion exists among database textbooks as to the goal of normalization as well as to which normal form a designer should aspire. This article discusses such discrepancies with the intention of simplifying normalization for both teacher and student. This author's industry and classroom experiences indicate such simplification yields quicker…

  18. Beyond the Normalized Difference Vegetation Index (NDVI): Developing a Natural Space Index for population-level health research.

    Science.gov (United States)

    Rugel, Emily J; Henderson, Sarah B; Carpiano, Richard M; Brauer, Michael

    2017-08-29

    Natural spaces can provide psychological benefits to individuals, but population-level epidemiologic studies have produced conflicting results. Refining current exposure-assessment methods is necessary to advance our understanding of population health and to guide the design of health-promoting urban forms. The aim of this study was to develop a comprehensive Natural Space Index that robustly models potential exposure based on the presence, form, accessibility, and quality of multiple forms of greenspace (e.g., parks and street trees) and bluespace (e.g., oceans and lakes). The index was developed for greater Vancouver, Canada. Greenness presence was derived from remote sensing (NDVI/EVI); forms were extracted from municipal and private databases; and accessibility was based on restrictions such as private ownership. Quality appraisals were conducted for 200 randomly sampled parks using the Public Open Space Desktop Appraisal Tool (POSDAT). Integrating these measures in GIS, exposure was assessed for 60,242 postal codes using 100- to 1,600-m buffers based on hypothesized pathways to mental health. A single index was then derived using principal component analysis (PCA). Comparing NDVI with alternate approaches for assessing natural space resulted in widely divergent results, with quintile rankings shifting for 22-88% of postal codes, depending on the measure. Overall park quality was fairly low (mean of 15 on a scale of 0-45), with no significant difference seen by neighborhood-level household income. The final PCA identified three main sets of variables, with the first two components explaining 68% of the total variance. The first component was dominated by the percentages of public and private greenspace and bluespace and public greenspace within 250m, while the second component was driven by lack of access to bluespace within 1 km. Many current approaches to modeling natural space may misclassify exposures and have limited specificity. The Natural Space Index

  19. Diffusion-weighted MR imaging of upper abdominal organs at different time points: Apparent diffusion coefficient normalization using a reference organ.

    Science.gov (United States)

    Song, Ji Soo; Kwak, Hyo Sung; Byon, Jung Hee; Jin, Gong Yong

    2017-05-01

    To compare the apparent diffusion coefficient (ADC) of upper abdominal organs acquired at different time points, and to investigate the usefulness of normalization. We retrospectively evaluated 58 patients who underwent three rounds of magnetic resonance (MR) imaging including diffusion-weighted imaging of the upper abdomen. MR examinations were performed using three different 3.0 Tesla (T) and one 1.5T systems, with variable b value combinations and respiratory motion compensation techniques. The ADC values of the upper abdominal organs from three different time points were analyzed, using the ADC values of the paraspinal muscle (ADCpsm ) and spleen (ADCspleen ) for normalization. Intraclass correlation coefficients (ICC) and comparison of dependent ICCs were used for statistical analysis. The ICCs of the original ADC and ADCpsm showed fair to substantial agreement, while ADCspleen showed substantial to almost perfect agreement. The ICC of ADCspleen of all anatomical regions showed less variability compared with that of the original ADC (P measurement of the upper abdominal organs in different MR systems at different time points and could be regarded as an imaging biomarker for future multicenter, longitudinal studies. 5 J. MAGN. RESON. IMAGING 2017;45:1494-1501. © 2016 International Society for Magnetic Resonance in Medicine.

  20. The Difference in the Profile of Working Memory, Auditory Working Memory, and Spatial Working Memory between Drug, Stimulant, and Methadone Abusers and Normal People

    Directory of Open Access Journals (Sweden)

    Ahmad Alipour

    2015-06-01

    Full Text Available Objective: The present study was an attempt to examine the difference in the profile of working memory, auditory working memory, and spatial working memory between drug, stimulant, and methadone abusers and normal people. Method: This study was a causal-comparative one with between-group comparison methodology. All the individuals addicted to opiates, stimulants, and methadone who had referred to Khomeini treatment centers of the city from September 2013 to February 2014 constituted the statistical population of the study. The number of 154 abusers (54 drug abusers, 50 stimulant abusers, and 50 methadone abusers and the number of 50 normal participants were chosen as the sample of the study by purposive sampling method. The participants responded to Wechsler Memory Scale—third edition (WMS-III. Results: There was a significant difference between the normal group and drug, stimulant, and methadone abusers in terms of working memory, auditory working memory, and spatial working memory. Conclusion: Drug and stimulant use leads to sustained damage in cognitive processes such as working memory. However, research indicates that these cognitive processes will improve with the passage of time.

  1. Texture analysis of collagen second-harmonic generation images based on local difference local binary pattern and wavelets differentiates human skin abnormal scars from normal scars.

    Science.gov (United States)

    Liu, Yao; Zhu, Xiaoqin; Huang, Zufang; Cai, Jianyong; Chen, Rong; Xiong, Shuyuan; Chen, Guannan; Zeng, Haishan

    2015-01-01

    Quantitative methods for noninvasive diagnosis of scars are a challenging issue in medicine. This work aims to implement a texture analysis method for quantitatively discriminating abnormal scars from normal scars based on second-harmonic generation (SHG) images. A local difference local binary pattern (LD-LBP) operator combined with a wavelet transform was explored to extract diagnosis features from scar SHG images that were related to the alteration in collagen morphology. Based on the quantitative parameters including the homogeneity, directional and coarse features in SHG images, the scar collagen SHG images were classified into normal or abnormal scars by a support vector machine classifier in a leave-one-out cross-validation procedure. Our experiments and data analyses demonstrated apparent differences between normal and abnormal scars in terms of their morphological structure of collagen. By comparing with gray level co-occurrence matrix, wavelet transform, and combined basic local binary pattern and wavelet transform with respect to the accuracy and receiver operating characteristic analysis, the method proposed herein was demonstrated to achieve higher accuracy and more reliable classification of SHG images. This result indicated that the extracted texture features with the proposed method were effective in the classification of scars. It could provide assistance for physicians in the diagnostic process.

  2. Comparative study of two different Bombesin derivates labeled with {sup 111}In and biodistribution in normal mice

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Ricardo S.; Alcarde, Lais F.; Correa, Beatriz L.; Massicano, Adriana V.F.; Couto, Renata M.; Mengatti, Jair; Araujo, Elaine B. de, E-mail: ricardooliveira@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    Nuclear medicine is a medical speciality that uses radioactive compounds (radiopharmaceuticals), consisting of a substrate and a radioactive isotope, for diagnostic. Among the peptides of interest for Nuclear Medicine, bombesin (BBN), a 14 amino acid neuropeptide analog of human gastrin-releasing peptide, is one of the highlights. This is a comparative study aiming to establish the best condition to radiolabel two BBN derivatives, (DTPA-Phe-Gly{sub 5}-BBN{sub (6-14)}) and (DTPA-Phe-Gly{sub 2}-BBN{sub (6-14})) with 111-indium. Specific objectives of this study were evaluate a good condition of radiolabelling in search excellent specific activity the bombesin derivatives and determinate the biodistribution in health mice model. Ten micrograms (10μg) of the derivative DTPA-Phe-Gly2-BBN (6-14) was labeled with 18.5 MBq (0.5 mCi) of {sup 111}InCl{sub 3} at 25°C for different times (5, 15 and 30 minutes). The best condition was applied to peptide mass variation (10, 5, 2.5, 1, 0.5, 0.25 and 0.1 μg), keeping all other parameters fixed. Finally, the influence of {sup 111}InCl{sub 3} activity in the radiolabeling process (18.5, 37, 55.5, 74, 185 MBq) was evaluated. The best conditions were repeated for the second derivate, DTPA-Phe-Gly{sub 5}-BBN{sub (6-14}). The radiochemical purity was assessed by thin layer chromatography (TLC), using 0.2 M EDTA pH 5 as solvent, and high performance liquid chromatography (HPLC) with a C18 column with linear gradient 10% A to 90% A (v/v) (A: 0,1% of TFA in CH3CN; B: 0,1% of TFA in H2O) at a flow rate of 1 mL/minute for 15 minutes. Considering the reaction time, the higher radiochemical purity was obtained when 10μg of the peptide was labeled with 18.5 MBq (0.5 mCi) of {sup 111}In for 15 minutes at 25°C (97.33 ± 0.50%, n=3). In the mass variation study, the best results of radiochemical purity were obtained when 10 μg of the peptide was employed (97.69 ± 0.4%, n = 4). Finally, the maximum specific activity of the radiolabelled

  3. Modulation of late sodium current by Ca2+, calmodulin, and CaMKII in normal and failing dog cardiomyocytes: similarities and differences.

    Science.gov (United States)

    Maltsev, Victor A; Reznikov, Vitaliy; Undrovinas, Nidas A; Sabbah, Hani N; Undrovinas, Albertas

    2008-04-01

    Augmented and slowed late Na(+) current (I(NaL)) is implicated in action potential duration variability, early afterdepolarizations, and abnormal Ca(2+) handling in human and canine failing myocardium. Our objective was to study I(NaL) modulation by cytosolic Ca(2+) concentration ([Ca(2+)](i)) in normal and failing ventricular myocytes. Chronic heart failure was produced in 10 dogs by multiple sequential coronary artery microembolizations; 6 normal dogs served as a control. I(NaL) fine structure was measured by whole cell patch clamp in ventricular myocytes and approximated by a sum of fast and slow exponentials produced by burst and late scattered modes of Na(+) channel gating, respectively. I(NaL) greatly enhanced as [Ca(2+)](i) increased from "Ca(2+) free" to 1 microM: its maximum density increased, decay of both exponentials slowed, and the steady-state inactivation (SSI) curve shifted toward more positive potentials. Testing the inhibition of CaMKII and CaM revealed similarities and differences of I(NaL) modulation in failing vs. normal myocytes. Similarities include the following: 1) CaMKII slows I(NaL) decay and decreases the amplitude of fast exponentials, and 2) Ca(2+) shifts SSI rightward. Differences include the following: 1) slowing of I(NaL) by CaMKII is greater, 2) CaM shifts SSI leftward, and 3) Ca(2+) increases the amplitude of slow exponentials. We conclude that Ca(2+)/CaM/CaMKII signaling increases I(NaL) and Na(+) influx in both normal and failing myocytes by slowing inactivation kinetics and shifting SSI. This Na(+) influx provides a novel Ca(2+) positive feedback mechanism (via Na(+)/Ca(2+) exchanger), enhancing contractions at higher beating rates but worsening cardiomyocyte contractile and electrical performance in conditions of poor Ca(2+) handling in heart failure.

  4. Laser and Non-Coherent Light Effect on Peripheral Blood Normal and Acute Lymphoblastic Leukemic Cells by Using Different Types of Photosensitizers

    Science.gov (United States)

    El Batanouny, Mohamed H.; Khorshid, Amira M.; Arsanyos, Sonya F.; Shaheen, Hesham M.; Abdel Wahab, Nahed; Amin, Sherif N.; El Rouby, Mahmoud N.; Morsy, Mona I.

    2010-04-01

    Photodynamic therapy (PDT) is a novel treatment modality of cancer and non-cancerous conditions that are generally characterized by an overgrowth of unwanted or abnormal cells. Irradiation of photosensitizer loaded cells or tissues leads via the photochemical reactions of excited photosensitizer molecules to the production of singlet oxygen and free radicals, which initiate cell death. Many types of compounds have been tested as photosensitizers, such as methylene blue (MB) and photopherin seemed to be very promising. This study involved 26 cases of acute lymphoblastic leukemia and 15 normal volunteers as a control group. The cell viability was measured by Light microscope and flowcytometer. Mode of cell death was detected by flowcytometer and electron microscope in selected cases. The viability percentage of normal peripheral blood mononuclear cells (PBMC) incubated with methylene blue (MB) alone or combined with photo irradiation with diode laser (as measured by light microscope) was significantly lower than that of untreated cases either measured after 1 hour (pdecrease in viability was more enhanced with increasing the incubation time. For normal cells incubated with photopherin either for 1/2 an hour or 1 hour, there was a weak cytotoxic effect compared to the effect on untreated cells. There was a significant decrease in viability percentage of cells incubated with photopherin either for 1/2 an hour or 1 hour and photoirradiated with He:Ne laser compared to normal untreated cells. The decrease in the cell viability percentage was significantly lower with the use of PDT (photopherin and He:Ne laser ) compared to either photopherin alone or He:Ne laser alone. The decrease in viability was more enhanced with increasing the incubation time. The same effects reported on normal cells were detected on leukemic cells on comparing different methods used. However a more pronounced decrease in cell viability was detected. The most efficient ways of decreasing viability

  5. Changes in the metabolic footprint of placental explant-conditioned medium cultured in different oxygen tensions from placentas of small for gestational age and normal pregnancies.

    LENUS (Irish Health Repository)

    Horgan, R P

    2012-01-31

    Being born small for gestational age (SGA) confers significantly increased risks of perinatal morbidity and mortality. Accumulating evidence suggests that an SGA fetus results from a poorly perfused and abnormally developed placenta. Some of the placental features seen in SGA, such as abnormal cell turnover and impaired nutrient transport, can be reproduced by culture of placental explants in hypoxic conditions. Metabolic footprinting offers a hypothesis-generating strategy to investigate factors absorbed by and released from this tissue in vitro. Previously, metabolic footprinting of the conditioned culture media has identified differences in placental explants cultured under normoxic and hypoxic conditions and between normal pregnancies and those complicated by pre-eclampsia. In this study we aimed to examine the differences in the metabolic footprint of placental villous explants cultured at different oxygen (O(2)) tensions between women who deliver an SGA baby (n = 9) and those from normal controls (n = 8). Placental villous explants from cases and controls were cultured for 96 h in 1% (hypoxic), 6% (normoxic) and 20% (hyperoxic) O(2). Metabolic footprints were analysed by Ultra Performance Liquid Chromatography coupled to an electrospray hybrid LTQ-Orbitrap Mass Spectrometry (UPLC-MS). 574 metabolite features showed significant difference between SGA and normal at one or more of the oxygen tensions. SGA explant media cultured under hypoxic conditions was observed, on a univariate level, to exhibit the same metabolic signature as controls cultured under normoxic conditions in 49% of the metabolites of interest, suggesting that SGA tissue is acclimatised to hypoxic conditions in vivo. No such behaviour was observed under hyperoxic culture conditions. Glycerophospholipid and tryptophan metabolism were highlighted as areas of particular interest.

  6. Effect Of 2% Chlorhexidine Application on the Bonding Effectiveness of Different Dentin Adhesives in Normal and Caries Affected Dentin in Primary Teeth: an in Vitro Study

    Directory of Open Access Journals (Sweden)

    Elham Farrokh gisour

    2016-03-01

    Full Text Available Background and Objective:The bond strength of adhesives to dentin is one of the most important parameters for evaluating the efficacy of dental adhesives.The aim of this study was to evaluate the effect of 2% chlorhexidine on 24 hour shear bond strength of different adhesives in normal and caries affected dentin in primary teeth. Materials and Methods:Sixty extracted human primary molars were selected and sectioned in two halves and after preparing a flat surface of superficial dentin, were randomly divided into 12 groups; according to dentin condition (normal and caries affected, type of adhesive (Clearfil SE Bond [SE], Clearfil S3Bond [S3 ], Single Bond2 [S2 ] and treatment (with chlorhexidine [2%CHX] and without 2%CHX . In S2 group, after etching, CHX were applied and in SE and S3 groups were applied before use of bonding agents. Then composite was attached to the prepared surfaces and cured. The samples were maintained in distilled water at 37 ˚C for 24 hours and then thermocycled. Shear bond strength was calculated. Mode of failures was examined by stereomicroscope. Data were analyzed using t-test and ANOVA with p<0.05 as the level of significance. Results:There were no significant differences in shear bond strength between the different types of adhesives (P=0.9, use of CHX (P=0.22 and type of dentin (P=0.9. The dominant fracture pattern was mixed. Conclusion:2% CHX had no effect on 24 hour shear bond strength of adhesive systems to normal and caries affected dentin in primary teeth.

  7. Metabolism of sup 3 H- and sup 14 C-labeled glutamate, proline, and alanine in normal and adrenalectomized rats using different sites of tracer administration and sampling

    Energy Technology Data Exchange (ETDEWEB)

    Said, H.M.; Chenoweth, M.; Dunn, A. (Univ. of Southern California, Los Angeles (USA))

    1989-08-01

    Alanine, glutamate and proline labeled with 14C and 3H were infused into fasted normal and adrenalectomized rats. Alanine was administered by the A-V mode (arterial administration-venous sampling), and glutamate and proline by both the A-V and V-A (venous administration-arterial sampling) modes. The kinetics of 14C alanine and 14C glutamate differed markedly from those of the tritium-labeled compounds, but there was little difference in the kinetics of 3H and 14C proline. The replacement rate calculated from the A-V mode for glutamate was about half that obtained in the V-A mode, but there was little difference with proline. The masses of the amino acids (total content of amino acids in the body) were calculated from the washout curves of the tritium-labeled compounds after the infusion of tracer was terminated. The masses for the normal rats were 407 mumol/kg for alanine, 578 mumol/kg for glutamate and 296 mumol/kg for proline. The so-called distribution spaces calculated conventionally from total masses and the amino acid concentrations in plasma are much greater than the volume of the body, reflecting the fact that amino acid concentrations in tissues greatly exceed those in plasma. Adrenalectomy markedly affected the kinetics of the three amino acids, and their replacement rates were greatly reduced. The proline and glutamate masses were reduced by at least one half, while that of alanine was unchanged. Adrenalectomy markedly reduced the conversion of proline to glutamate. The hydrocortisone regimen used in this study restored the metabolism of alanine and glutamate to normal, but had no effect on that of proline.

  8. A Preliminary Study on Racial Differences in HMOX1, NFE2L2, and TGFβ1 Gene Polymorphisms and Radiation-Induced Late Normal Tissue Toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Alam, Asim [Department of Radiation Oncology, Virginia Commonwealth University, Richmond, Virginia (United States); Mukhopadhyay, Nitai D. [Department of Biostatistics, Virginia Commonwealth University, Richmond, Virginia (United States); Ning, Yi [Department of Family Medicine and Population Health, Virginia Commonwealth University, Richmond, Virginia (United States); Reshko, Leonid B.; Cardnell, Robert J.G.; Alam, Omair; Rabender, Christopher S.; Yakovlev, Vasily A.; Walker, Linda; Anscher, Mitchell S. [Department of Radiation Oncology, Virginia Commonwealth University, Richmond, Virginia (United States); Mikkelsen, Ross B., E-mail: rmikkels@vcu.edu [Department of Radiation Oncology, Virginia Commonwealth University, Richmond, Virginia (United States)

    2015-10-01

    Purpose: This study tested whether racial differences in genetic polymorphisms of 4 genes involved in wound repair and response to radiation can be used to predict the occurrence of normal tissue late effects of radiation therapy and indicate potential therapeutic targets. Methods and Materials: This prospective study examined genetic polymorphisms that modulate the expression of 4 genes involved in inflammation and fibrosis and response to radiation (HMOX1, NFE2L2, NOS3, and TGFβ1). DNA from blood samples of 179 patients (∼80% breast and head and neck) collected at the time of diagnosis by their radiation oncologist as exhibiting late normal tissue toxicity was used for the analysis. Patient demographics were as follows: 56% white, 43% African American, 1% other. Allelic frequencies of the different polymorphisms of the participants were compared with those of the general American population stratified by race. Twenty-six additional patients treated with radiation, but without toxicity at 3 months or later after therapy, were also analyzed. Results: Increased frequency of a long GT repeat in the HMOX1 promoter was associated with late effects in both African American and white populations. The single nucleotide polymorphisms (SNP) rs1800469 in the TGFβ1 promoter and the rs6721961 SNP in the NFE2L2 promoter were also found to significantly associate with late effects in African Americans but not whites. A combined analysis of these polymorphisms revealed that >90% of African American patients with late effects had at least 1 of these minor alleles, and 58% had 2 or more. No statistical significance was found relating the studied NOS3 polymorphisms and normal tissue toxicity. Conclusions: These results support a strong association between wound repair and late toxicities of radiation. The presence of these genetic risk factors can vary significantly among different ethnic groups, as demonstrated for some of the SNPs. Future studies should account for the

  9. The differences in ultrasound and clinicopathological features between basal-like and normal-like subtypes of triple negative breast cancer.

    Directory of Open Access Journals (Sweden)

    Ziyao Li

    Full Text Available The aim of this study was to identify the ultrasound features and clinicopathological characteristics of basal-like subtype of triple negative breast cancers (TNBCs.This study was approved by the ethical board of the Second Affiliated Hospital of Harbin Medical University. The patients' clinicopathological information was available. The ultrasound features of 62 tumors from 62 TNBC patients were interpreted. The immunohistochemical results of cytokertain5/6 (CK5/6 and Epidermal Growth Factor Receptor (EGFR were used to classify the tumor into basal-like and normal-like groups. The association of the ultrasound features interpreted by experienced ultrasound doctors with the immunohistochemical classification was studied.Of the 62 TNBC cases, 42 (67.7% exhibited the basal-like phenotype and 20 (32.3% exhibited the normal-like phenotype based on the immunohistochemical CK5/6 and EGFR markers. Of all the tumors, 90.3% were invasive carcinomas. The basal-like tumors were significantly associated with a maximum diameter on ultrasound of more than 20 mm (36, 85.7% (P = 0.0014. The normal-like tumors usually exhibited lateral shadows (15, 75% (P = 0.0115 as well as microlobulated margins (12, 60% (P = 0.0204 compared to the basal-like subtype. Other ultrasound features showed no significant differences between the two groups.Although ultrasound cannot yet be used to differentiate between the basal-like subtype and normal-like subtype of TNBC, ultrasound can be used to provide some useful information to the clinicians.

  10. The Hamburg Ocean-Atmosphere Parameters and Fluxes from Satellite Data (HOAPS): A climatological atlas of satellite-derived air-sea interaction parameters over the world oceans

    Digital Repository Service at National Institute of Oceanography (India)

    Grassl, H.; Jost, V.; Schulz, J.; RameshKumar, M.R.; Bauer, P.; Schluessel, P.

    temperature, specific humidity at air and sea surface temperature, difference in humidity, Dalton number, wind speed and the air sea fluxes such as latent heat, sensible heat and longwave radiation. The atlas also provides the hydrological cycle parameters..., the humidity difference, wind speed, the Dalton num- ber used for the parameterisation of latent heat flux, latent heat flux, sensible heat flux, net longwave radiation at the surface, evaporation, rainfall, and the freshwater flux computed as their difference...

  11. [Evaluation of image quality using the normalized-rank approach for primary class liquid-crystal display (LCD) monitors with different colors and resolution].

    Science.gov (United States)

    Kuroki, Hidefumi; Katayama, Reiji; Sakaguchi, Taro; Maeda, Takashi; Morishita, Junji; Hayabuchi, Naofumi

    2010-11-20

    The purposes of this study were to evaluate the image quality of five types of liquid-crystal display (LCD) monitors by utilizing the normalized-rank approach and to investigate the effect of LCD monitor specifications, such as display colors, luminance, and resolution, on the evaluators' ranking. The LCD monitors used in this study were 2, 3 and 5 mega-pixel monochrome LCD monitors, and 2 and 3 mega-pixel color LCD monitors (Eizo Nanao Corporation). All LCD monitors were calibrated to the grayscale standard display function (GSDF) with different maximum luminance (recommended luminance) settings. Also, four kinds of radiographs were used for observer study based on the normalized-rank approach: three adult chest radiographs, three pediatric chest radiographs, three ankle joint radiographs, and four double-contrasted upper gastrointestinal radiographs. Ten radiological technologists participated in the observer study. Monochrome LCD monitors exhibited superior ranking with statistically significant differences (pLCD monitors in all kinds of radiographs. The major difference between monochrome and color monitors was luminance. Therefore, it is considered that the luminance of LCD monitors affects observers' evaluations based on image quality. Moreover, in the case of radiographs that include high frequency image components, the monitor resolution also affects the evaluation. In clinical practice, it is necessary to optimize the luminance and choose appropriate LCD monitors for diagnostic images.

  12. Assessing the Impacts of the 2009/2010 Drought on Vegetation Indices, Normalized Difference Water Index, and Land Surface Temperature in Southwestern China

    Directory of Open Access Journals (Sweden)

    Xiaoqiang Zhang

    2017-01-01

    Full Text Available Droughts are projected to increase in severity and frequency on both regional and global scales. Despite the increasing occurrence and intensity of the 2009/2010 drought in southwestern China, the impacts of drought on vegetation in this region remain unclear. We examined the impacts of the 2009/2010 drought in southwestern China on vegetation by calculating the standardized anomalies of Normalized Difference Vegetation Index (NDVI, Enhanced Vegetation Index (EVI, Normalized Difference Water Index (NDWI, and Land Surface Temperature (LST. The standardized anomalies of NDVI, EVI, and NDWI exhibited positively skewed frequency distributions, while the standardized anomalies of LST exhibited a negatively skewed frequency distribution. These results implied that the NDVI, EVI, and NDWI declined, while LST increased in the 2009/2010 drought-stricken vegetated areas during the drought period. The responses of vegetation to the 2009/2010 drought differed substantially among biomes. Savannas, croplands, and mixed forests were more vulnerable to the 2009/2010 drought than deciduous forest and grasslands, while evergreen forest was resistant to the 2009/2010 drought in southwestern China. We concluded that the 2009/2010 drought had negative impacts on vegetation in southwestern China. The resulting assessment on the impacts of drought assists in evaluating and mitigating its adverse effects in southwestern China.

  13. A Dose-Response Strategy Reveals Differences between Normal-Weight and Obese Men in Their Metabolic and Inflammatory Responses to a High-Fat Meal.

    OpenAIRE

    Schwander, Flurina; Kopf-Bolanz, Katrin A.; Buri, Caroline; Portmann, Reto; Egger, Lotti; Chollet, Magali; McTernan, P.G.; Piya, Milan K.; Gijs, Martin A. M.; Vionnet, Nathalie; Pralong, Franc̨ois; Laederach, Kurt; Vergeres, Guy

    2014-01-01

    A dose-response strategy may not only allow investigation of the impact of foods and nutrients on human health but may also reveal differences in the response of individuals to food ingestion based on their metabolic health status. In a randomized crossover study, we challenged 19 normal-weight (BMI: 20-25 kg/m(2)) and 18 obese (BMI: >30 kg/m(2)) men with 500, 1000, and 1500 kcal of a high-fat (HF) meal (60.5% energy from fat). Blood was taken at baseline and up to 6 h postprandially and a...

  14. EGFR signaling pathways are wired differently in normal 184A1L5 human mammary epithelial and MDA-MB-231 breast cancer cells.

    Science.gov (United States)

    Speth, Zachary; Islam, Tanzila; Banerjee, Kasturi; Resat, Haluk

    2017-03-29

    Because of differences in the downstream signaling patterns of its pathways, the role of the human epidermal growth factor family of receptors (HER) in promoting cell growth and survival is cell line and context dependent. Using two model cell lines, we have studied how the regulatory interaction network among the key proteins of HER signaling pathways may be rewired upon normal to cancerous transformation. We in particular investigated how the transcription factor STAT3 and several key kinases' involvement in cancer-related signaling processes differ between normal 184A1L5 human mammary epithelial (HME) and MDA-MB-231 breast cancer epithelial cells. Comparison of the responses in these cells showed that normal-to-cancerous cellular transformation causes a major re-wiring of the growth factor initiated signaling. In particular, we found that: i) regulatory interactions between Erk, p38, JNK and STAT3 are triangulated and tightly coupled in 184A1L5 HME cells, and ii) STAT3 is only weakly associated with the Erk-p38-JNK pathway in MDA-MB-231 cells. Utilizing the concept of pathway substitution, we predicted how the observed differences in the regulatory interactions may affect the proliferation/survival and motility responses of the 184A1L5 and MDA-MB-231 cells when exposed to various inhibitors. We then validated our predictions experimentally to complete the experiment-computation-experiment iteration loop. Validated differences in the regulatory interactions of the 184A1L5 and MDA-MB-231 cells indicated that instead of inhibiting STAT3, which has severe toxic side effects, simultaneous inhibition of JNK together with Erk or p38 could be a more effective strategy to impose cell death selectively to MDA-MB-231 cancer cells while considerably lowering the side effects to normal epithelial cells. Presented analysis establishes a framework with examples that would enable cell signaling researchers to identify the signaling network structures which can be used to

  15. Birkhoff normalization

    NARCIS (Netherlands)

    Broer, H.; Hoveijn, I.; Lunter, G.; Vegter, G.

    2003-01-01

    The Birkhoff normal form procedure is a widely used tool for approximating a Hamiltonian systems by a simpler one. This chapter starts out with an introduction to Hamiltonian mechanics, followed by an explanation of the Birkhoff normal form procedure. Finally we discuss several algorithms for comput

  16. Raman spectroscopy analysis of differences in composition of spent culture media of in vitro cultured preimplantation embryos isolated from normal and fat mice dams.

    Science.gov (United States)

    Fabian, Dušan; Kačmarová, Martina; Kubandová, Janka; Čikoš, Štefan; Koppel, Juraj

    2016-06-01

    The aim of the present study was to compare overall patterns of metabolic activity of in vitro cultured preimplantation embryos isolated from normal and fat mice dams by means of non-invasive profiling of spent culture media using Raman spectroscopy. To produce females with two different types of body condition (normal and fat), a previously established two-generation model was used, based on overfeeding of experimental mice during prenatal and early postnatal development. Embryos were isolated from spontaneously ovulating and naturally fertilized dams at the 2-cell stage of development and cultured to the blastocyst stage in synthetic oviductal medium KSOMaa. Embryos from fat mice (displaying significantly elevated body weight and fat) showed similar developmental capabilities in vitro as embryos isolated from normal control dams (displaying physiological body weight and fat). The results show that alterations in the composition of culture medium caused by the presence of developing mouse preimplantation embryos can be detected using Raman spectroscopy. Metabolic activity of embryos was reflected in evident changes in numerous band intensities in the 1620-1690cm(-1) (amide I) region and in the 1020-1140cm(-1) region of the Raman spectrum for KSOMaa. Moreover, multivariate analysis of spectral data proved that the composition of proteins and other organic compounds in spent samples obtained after the culture of embryos isolated from fat dams was different from that in spent samples obtained after the culture of embryos from control dams. This study demonstrates that metabolic activity of cultured preimplantation embryos might depend on the body condition of their donors.

  17. Photosynthesis-dependent and -independent responses of stomata to blue, red and green monochromatic light: differences between the normally oriented and inverted leaves of sunflower.

    Science.gov (United States)

    Wang, Yin; Noguchi, Ko; Terashima, Ichiro

    2011-03-01

    The effects of growth light environment on stomatal light responses were analyzed. We inverted leaves of sunflower (Helianthus annuus) for 2 weeks until their full expansion, and measured gas exchange properties of the adaxial and abaxial sides separately. The sensitivity to light assessed as the increase in stomatal conductance was generally higher in the abaxial stomata than in the adaxial stomata, and these differences could not be completely changed by the inversion treatment. We also treated the leaves with DCMU to inhibit photosynthesis and evaluated the photosynthesis-dependent and -independent components of stomatal light responses. The red light response of stomata in both normally oriented and inverted leaves relied only on the photosynthesis-dependent component. The blue light response involved both the photosynthesis-dependent and photosynthesis-independent components, and the relative contributions of the two components differed between the normally oriented and inverted leaves. A green light response was observed only in the abaxial stomata, which also involved the photosynthesis-dependent and photosynthesis-independent components, strongly suggesting the existence of a green light receptor in sunflower leaves. Moreover, acclimation of the abaxial stomata to strong direct light eliminated the photosynthesis-independent component in the green light response. The results showed that stomatal responses to monochromatic light change considerably in response to growth light environment, although some of these responses appear to be determined inherently. © The Author 2011. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.

  18. [Analysis of difference of serum proteins between patients with cirrhosis and normal people by capillary electrophoresis with light-emitting diode induced fluorescence detection].

    Science.gov (United States)

    Bao, Rihuang; Fan, Qingjie; Song, Long; Yao, Yuan; Gao, Weiping

    2015-02-01

    An experimental method of protein separation by capillary electrophoresis coupled with light-emitting diode induced fluorescence detection (CE-LED-IF) was established. The fluorescein isothiocyanate (FITC) was used as fluorescent dye in this method. An optical fiber was inserted into the separation capillary and was suited right at the detection window which was used to transmit the excitation light of LED. The efficiency of the light of LED and the detection limits of FITC was improved. The difference of serum proteins between the patients with cirrhosis and normal people was detected by this method. A series of effects on CE were studied, such as the wavelength of the light source and filter, the concentration and pH of the running buffer and the separation voltage. Then the optimized method was established for the determination of the serum proteins. The repeatability of CE-LED-IF was tested and the RSDs of migration time and peak area were 1. 14% and 3. 23% , respectively. The results showed that there were two specific peaks in cirrhosis serum. The differences between the patients with cirrhosis and normal people having statistical significance indicated that these proteins might be the potential biomarkers for cirrhosis diagnosis. The developed CE-LED-IF method proved suitable for the separation of serum proteins with good repeatability and supportable for the clinical diagnosis of cirrhosis.

  19. Granulocyte-macrophage colony-stimulating factor levels in amniotic fluid before the onset of labor and during labor do not differ in normal pregnancies.

    Science.gov (United States)

    Hayashi, Masatoshi; Sohma, Ryoichi; Sumioka, Yumiko; Inaba, Noriyuki

    2006-01-01

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) at the implantation site may regulate invasion and differentiation of placental trophoblast. We evaluated whether GM-CSF levels in amniotic fluid during labor contributing to subsequent delivery differed from those before the onset of labor in normal pregnancies. This study enrolled 36 Japanese women experiencing normal pregnancies with single fetuses who had no infection. Of these pregnancies, 18 were women during labor that led to subsequent term delivery (labors). The other 18 were women without labor underwent cesarean section (controls). These two groups (18 labors and 18 controls) were compared. The average gestational age at entry was 38-39 weeks of gestation. The women's ages and gestational ages did not differ significantly between the two groups. Amniotic fluid was collected and the GM-CSF levels were compared between two groups. The GM-CSF level was determined by the enzyme-linked immunosorbent assay method. There was no significant increase in GM-CSF levels in amniotic fluid during labor compared with that before the onset of labor. The GM-CSF in amniotic fluid may not promote the onset of labor at term and/or term labor contributing to subsequent delivery may not induce the production and secretion of GM-CSF into amniotic cavity.

  20. Effect of the normalized prescription isodose line on the magnitude of Monte Carlo vs. pencil beam target dose differences for lung stereotactic body radiotherapy.

    Science.gov (United States)

    Zheng, Dandan; Zhang, Qinghui; Liang, Xiaoying; Zhu, Xiaofeng; Verma, Vivek; Wang, Shuo; Zhou, Sumin

    2016-07-08

    In lung stereotactic body radiotherapy (SBRT) cases, the pencil beam (PB) dose calculation algorithm is known to overestimate target dose as compared to the more accurate Monte Carlo (MC) algorithm. We investigated whether changing the normalized prescription isodose line affected the magnitude of MC vs. PB target dose differences. Forty-eight patient plans and twenty virtual-tumor phantom plans were studied. For patient plans, four alternative plans prescribed to 60%, 70%, 80%, and 90% isodose lines were each created for 12 patients who previously received lung SBRT treatments. Using 6 MV dynamic conformal arcs, the plans were individually optimized to achieve similar dose coverage and conformity for all plans of the same patient, albeit at the different prescription levels. These plans, having used a PB algorithm, were all recalculated with MC to compare the target dose differences. The relative MC vs. PB target dose variations were investigated by comparing PTV D95, Dmean, and D5 loss at the four prescription levels. The MC-to-PB ratio of the plan heterogeneity index (HI) was also evaluated and compared among different isodose levels. To definitively demonstrate the cause of the isodose line dependence, a simulated phantom study was conducted using simple, spherical virtual tumors planned with uniform block margins. The tumor size and beam energy were also altered in the phantom study to investigate the interplay between these confounding factors and the isodose line effect. The magnitude of the target dose overestimation by PB was greater for higher prescription isodose levels. The MC vs. PB reduction in the target dose coverage indices, D95 and V100 of PTV, were found to monotonically increase with increasing isodose lines from 60% to 90%, resulting in more pronounced target dose coverage deficiency at higher isodose prescription levels. No isodose level-dependent trend was observed for the dose errors in the target mean or high dose indices, Dmean or D5. The

  1. Evaluation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in Pyrus pyrifolia using different tissue samples and seasonal conditions.

    Science.gov (United States)

    Imai, Tsuyoshi; Ubi, Benjamin E; Saito, Takanori; Moriguchi, Takaya

    2014-01-01

    We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α, Glyceraldehyde-3-phosphate dehydrogenase, together with newly added genes Annexin, SAND and TIP41. A total of 17 primer combinations for these eight genes were evaluated using cDNAs synthesized from 16 tissue samples from four groups, namely: flower bud, flower organ, fruit flesh and fruit skin. Gene expression stabilities were analyzed using geNorm and NormFinder software packages or by ΔCt method. geNorm analysis indicated three best performing genes as being sufficient for reliable normalization of RT-qPCR data. Suitable reference genes were different among sample groups, suggesting the importance of validation of gene expression stability of reference genes in the samples of interest. Ranking of stability was basically similar between geNorm and NormFinder, suggesting usefulness of these programs based on different algorithms. ΔCt method suggested somewhat different results in some groups such as flower organ or fruit skin; though the overall results were in good correlation with geNorm or NormFinder. Gene expression of two cold-inducible genes PpCBF2 and PpCBF4 were quantified using the three most and the three least stable reference genes suggested by geNorm. Although normalized quantities were different between them, the relative quantities within a group of samples were similar even when the least stable reference genes were used. Our data suggested that using the geometric mean value of three reference genes for normalization is quite a reliable approach to evaluating gene expression by RT-qPCR. We propose that the initial evaluation of gene expression stability by ΔCt method, and subsequent evaluation by geNorm or NormFinder for limited number of superior gene candidates will be a practical way of finding out

  2. Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR in Baphicacanthus cusia under Hormonal Stimuli and UV Irradiation, and in Different Plant Organs

    Directory of Open Access Journals (Sweden)

    Yuxiang Huang

    2017-05-01

    Full Text Available Baphicacanthus cusia (Nees Bremek, the plant source for many kinds of drugs in traditional Chinese medicine, is widely distributed in South China, especially in Fujian. Recent studies about B. cusia mainly focus on its chemical composition and pharmacological effects, but further analysis of the plant's gene functions and expression is required to better understand the synthesis of its effective compounds. Real-time quantitative polymerase chain reaction (RT-qPCR is a powerful method for gene expression analysis. It is necessary to select a suitable reference gene for expression normalization to ensure the accuracy of RT-qPCR results. Ten candidate reference genes were selected from the transcriptome datasets of B. cusia in this study, and the expression stability was assessed across 60 samples representing different tissues and organs under various conditions, including ultraviolet (UV irradiation, hormonal stimuli (jasmonic acid methyl ester and abscisic acid, and in different plant organs. By employing different algorithms, such as geNorm, NormFinder, and BestKeeper, which are complementary approaches based on different statistical procedures, 18S rRNA was found to be the most stable gene under UV irradiation and hormonal stimuli, whereas ubiquitin-conjugating enzyme E2 was the best suitable gene for different plant organs. This novel study aimed to screen for suitable reference genes and corresponding primer pairs specifically designed for gene expression studies in B. cusia, in particular for RT-qPCR analyses.

  3. Diffusion tensor imaging of white and grey matter within the spinal cord of normal Beagle dogs: Sub-regional differences of the various diffusion parameters.

    Science.gov (United States)

    Yoon, Hakyoung; Park, Noh-Won; Ha, Yun-Mi; Kim, Jaehwan; Moon, Won-Jin; Eom, Kidong

    2016-09-01

    Diffusion tensor imaging (DTI) is an advanced diffusion weighted imaging technique that can identify early stage lesions and Wallerian degeneration within the spinal cord; these changes are difficult to recognise on conventional magnetic resonance imaging (MRI). The only DTI parameters previously investigated in dogs are fractional anisotropy and mean diffusivity (MD). The aim of this study was to evaluate multiple DTI parameters in sub-regional areas of the spinal cord in normal Beagles. All imaging data were obtained from the lumbar spinal cord (L1-L3) of ten normal dogs using a 3 Tesla MRI scanner. Transverse multi-shot echo planar imaging sequences (b values = 0 and 800 s/mm(2); 12 directions) were used for DTI. Regions of interest were selected from sub-regions of the white and grey matter, and from the whole spinal cord, in the transverse plane in all DTI maps. The DTI parameters in spinal cord sub-regions in the transverse plane were significantly different amongst the white matter, grey matter and whole spinal cord (P matter sub-regions (P grey matter may be useful for regional evaluation of the dog spinal cord.

  4. [How do risk patients with overweight/obesity differ in their health knowledge and behaviour from patients with normal weight? A study in primary medical care].

    Science.gov (United States)

    Küpper, B; Krause, P; Glaesmer, H; Wittchen, H-U

    2004-06-01

    Individual health-related behaviour patterns and lifestyles are strongly associated with the risk of suffering from cardiovascular diseases. Overweight (BMI 25 to 30 kg/m(2)) and obese patients (BMI > or = 30 kg/m(2)) are at particular risk to develop these diseases. Therefore, we investigated whether these patients are more aware of health-related issues and problems than normal-weight patient with data from the HYDRA study on 45,000 subjects. Health knowledge, problem awareness and health behaviour differed significantly among the examined patient groups (normal weight/overweight/obesity). The overweight and obese patients were aware of potential risk factors for various diseases (e. g. hypertension, diabetes); they recognized their own health-related problems and attended courses to change their problematic health behaviours more frequently. According to the patients' evaluations, however, these offers of courses are not very helpful. Changing the contents and implementations of health courses seems necessary to decrease costs and improve quality in the health care system on a long-term basis.

  5. A matrix clustering method to explore patterns of land-cover transitions in satellite-derived maps of the Brazilian Amazon

    Science.gov (United States)

    Müller-Hansen, Finn; Cardoso, Manoel F.; Dalla-Nora, Eloi L.; Donges, Jonathan F.; Heitzig, Jobst; Kurths, Jürgen; Thonicke, Kirsten

    2017-02-01

    Changes in land-use systems in tropical regions, including deforestation, are a key challenge for global sustainability because of their huge impacts on green-house gas emissions, local climate and biodiversity. However, the dynamics of land-use and land-cover change in regions of frontier expansion such as the Brazilian Amazon are not yet well understood because of the complex interplay of ecological and socioeconomic drivers. In this paper, we combine Markov chain analysis and complex network methods to identify regimes of land-cover dynamics from land-cover maps (TerraClass) derived from high-resolution (30 m) satellite imagery. We estimate regional transition probabilities between different land-cover types and use clustering analysis and community detection algorithms on similarity networks to explore patterns of dominant land-cover transitions. We find that land-cover transition probabilities in the Brazilian Amazon are heterogeneous in space, and adjacent subregions tend to be assigned to the same clusters. When focusing on transitions from single land-cover types, we uncover patterns that reflect major regional differences in land-cover dynamics. Our method is able to summarize regional patterns and thus complements studies performed at the local scale.

  6. Reducing the Impact of Sampling Bias in NASA MODIS and VIIRS Level 3 Satellite Derived IR SST Observations over the Arctic

    Science.gov (United States)

    Minnett, P. J.; Liu, Y.; Kilpatrick, K. A.

    2016-12-01

    Sea-surface temperature (SST) measurements by satellites in the northern hemisphere high latitudes confront several difficulties. Year-round prevalent clouds, effects near ice edges, and the relative small difference between SST and low-level cloud temperatures lead to a significant loss of infrared observations regardless of the more frequent polar satellite overpasses. Recent research (Liu and Minnett, 2016) identified sampling issues in the Level 3 NASA MODIS SST products when 4km observations are aggregated into global grids at different time and space scales, particularly in the Arctic, where a binary decision cloud mask designed for global data is often overly conservative at high latitudes and results in many gaps and missing data. This under sampling of some Arctic regions results in a warm bias in Level 3 products, likely a result of warmer surface temperature, more distant from the ice edge, being identified more frequently as cloud free. Here we present an improved method for cloud detection in the Arctic using a majority vote from an ensemble of four classifiers trained based on an Alternative Decision Tree (ADT) algorithm (Freund and Mason 1999, Pfahringer et. al. 2001). This new cloud classifier increases sampling of clear pixel by 50% in several regions and generally produces cooler monthly average SST fields in the ice-free Arctic, while still retaining the same error characteristics at 1km resolution relative to in situ observations. SST time series of 12 years of MODIS (Aqua and Terra) and more recently VIIRS sensors are compared and the improvements in errors and uncertainties resulting from better cloud screening for Level 3 gridded products are assessed and summarized.

  7. The impact of different background noises: effects on cognitive performance and perceived disturbance in employees with aided hearing impairment and normal hearing.

    Science.gov (United States)

    Hua, Håkan; Emilsson, Magnus; Kähäri, Kim; Widén, Stephen; Möller, Claes; Lyxell, Björn

    2014-10-01

    Health care professionals frequently meet employees with hearing impairment (HI) who experience difficulties at work. There are indications that the majority of these difficulties might be related to the presence of background noise. Moreover, research has also shown that high-level noise has a more detrimental effect on cognitive performance and self-rated disturbance in individuals with HI than low-level noise. The purpose of this study was to examine the impact of different types of background noise on cognitive performance and perceived disturbance (PD) in employees with aided HI and normal hearing. A mixed factorial design was conducted to examine the effect of noise in four experimental conditions. A total of 40 participants (21 men and 19 women) were recruited to take part in the study. The study sample consisted of employees with HI (n = 20) and normal hearing (n = 20). The group with HI had a mild-moderate sensorineural HI, and they were all frequent hearing-aid users. The current study was conducted by using four general work-related tasks (mental arithmetic, orthographic decoding, phonological decoding, and serial recall) in four different background conditions: (1) quiet, (2) office noise at 56 dBA, (3) daycare noise at 73.5 dBA, and (4) traffic noise at 72.5 dBA. Reaction time and the proportion of correct answers in the working tasks were used as outcome measures of cognitive performance. The Borg CR-10 scale was used to assess PD. Data collection occurred on two separate sessions, completed within 4 wk of each other. All tasks and experimental conditions were used in a counterbalanced order. Two-way analysis of variance with repeated measures was performed to analyze the results. To examine interaction effects, pairwise t-tests were used. Pearson correlation coefficients between reaction time and proportion of correct answers, and cognitive performance and PD were also calculated to examine the possible correlation between the different variables. No

  8. Quantitative structure-retardation factor relationship of protein amino acids in different solvent mixtures for normal-phase thin-layer chromatography.

    Science.gov (United States)

    Yousefinejad, Saeed; Honarasa, Fatemeh; Saeed, Negar

    2015-05-01

    A quantitative predictive/descriptive model was proposed for the retardation factors of protein amino acids in normal-phase thin-layer chromatography. The experimental retardation factors of 126 chromatographic mixtures (21 protein amino acids in different mobile phases) were used as the independent variable. The matrix of dependent variables of the model was built using structural descriptors of amino acids and empirical parameters of solvents of the applied mobile phases. After variable selection, a five-parametr model was proposed for the retardation factor of amino acids, which covered about 84 and 77% variance of data in training and cross-validation, respectively. The correlation coefficient of the external test set was 0.80, which shows the prediction potential of proposed model as well as its good applicability domain that was checked using a standardized residual-leverage plot. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Atmospheric COS measurements and satellite-derived vegetation fluorescence data to evaluate the terrestrial gross primary productivity of CMIP5 model

    Science.gov (United States)

    Peylin, Philippe; MacBean, Natasha; Launois, Thomas; Belviso, Sauveur; Cadule, Patricia; Maignan, Fabienne

    2016-04-01

    Predicting the fate of the ecosystem carbon stocks and their sensitivity to climate change strongly relies on our ability to accurately model the gross carbon fluxes, i.e. photosynthesis and respiration. The Gross Primary Productivity (GPP) simulated by the different terrestrial models used in CMIP5 show large differences however, not only in terms of mean value but also in terms of phase and amplitude, thus hampering accurate investigations into carbon-climate feedbacks. While the net C flux of an ecosystem (NEE) can be measured in situ with the eddy covariance technique, the GPP is not directly accessible at larger scales and usually estimates are based on indirect measurements combining different tracers. Recent measurements of a new atmospheric tracer, the Carbonyl sulphide (COS), as well as the global measurement of Solar Induced Fluorescence (SIF) from satellite instruments (GOSAT, GOME2) open a new window for evaluating the GPP of earth system models. The use of COS relies on the fact that it is absorbed by the leaves in a similar manner to CO2, while there seems to be nothing equivalent to respiration for COS. Following recent work by Launois et al. (ACP, 2015), there is a potential to evaluate model GPP from atmospheric COS and CO2 measurements, using a transport model and recent parameterizations for the non-photosynthetic sinks (oxic soils, atmospheric oxidation) and biogenic sources (oceans and anoxic soils) of COS. Vegetation uptake of COS is modeled as a linear function of GPP and the ratio of COS to CO2 rate of uptake by plants. For the fluorescence, recent measurements of SIF from space appear to be highly correlated with monthly variations of data-driven GPP estimates (Guanter et al., 2012), following a strong dependence of vegetation SIF on photosynthetic activity. These global measurements thus provide new indications on the timing of canopy carbon uptake. In this work, we propose a dual approach that combines the strength of both COS and SIF

  10. From skin to bulk: An adjustment technique for assimilation of satellite-derived temperature observations in numerical models of small inland water bodies

    Science.gov (United States)

    Javaheri, Amir; Babbar-Sebens, Meghna; Miller, Robert N.

    2016-06-01

    Data Assimilation (DA) has been proposed for multiple water resources studies that require rapid employment of incoming observations to update and improve accuracy of operational prediction models. The usefulness of DA approaches in assimilating water temperature observations from different types of monitoring technologies (e.g., remote sensing and in-situ sensors) into numerical models of in-land water bodies (e.g., lakes and reservoirs) has, however, received limited attention. In contrast to in-situ temperature sensors, remote sensing technologies (e.g., satellites) provide the benefit of collecting measurements with better X-Y spatial coverage. However, assimilating water temperature measurements from satellites can introduce biases in the updated numerical model of water bodies because the physical region represented by these measurements do not directly correspond with the numerical model's representation of the water column. This study proposes a novel approach to address this representation challenge by coupling a skin temperature adjustment technique based on available air and in-situ water temperature observations, with an ensemble Kalman filter based data assimilation technique. Additionally, the proposed approach used in this study for four-dimensional analysis of a reservoir provides reasonably accurate surface layer and water column temperature forecasts, in spite of the use of a fairly small ensemble. Application of the methodology on a test site - Eagle Creek Reservoir - in Central Indiana demonstrated that assimilation of remotely sensed skin temperature data using the proposed approach improved the overall root mean square difference between modeled surface layer temperatures and the adjusted remotely sensed skin temperature observations from 5.6°C to 0.51°C (i.e., 91% improvement). In addition, the overall error in the water column temperature predictions when compared with in-situ observations also decreased from 1.95°C (before assimilation

  11. On the Use of Global Flood Forecasts and Satellite-Derived Inundation Maps for Flood Monitoring in Data-Sparse Regions

    Directory of Open Access Journals (Sweden)

    Beatriz Revilla-Romero

    2015-11-01

    Full Text Available Early flood warning and real-time monitoring systems play a key role in flood risk reduction and disaster response decisions. Global-scale flood forecasting and satellite-based flood detection systems are currently operating, however their reliability for decision-making applications needs to be assessed. In this study, we performed comparative evaluations of several operational global flood forecasting and flood detection systems, using 10 major flood events recorded over 2012–2014. Specifically, we evaluated the spatial extent and temporal characteristics of flood detections from the Global Flood Detection System (GFDS and the Global Flood Awareness System (GloFAS. Furthermore, we compared the GFDS flood maps with those from NASA’s two Moderate Resolution Imaging Spectroradiometer (MODIS sensors. Results reveal that: (1 general agreement was found between the GFDS and MODIS flood detection systems, (2 large differences exist in the spatio-temporal characteristics of the GFDS detections and GloFAS forecasts, and (3 the quantitative validation of global flood disasters in data-sparse regions is highly challenging. Overall, satellite remote sensing provides useful near real-time flood information that can be useful for risk management. We highlight the known limitations of global flood detection and forecasting systems, and propose ways forward to improve the reliability of large-scale flood monitoring tools.

  12. Assessing the Sensitivity of Satellite-Derived Estimates of Ice Sheet Mass Balance to Regional Climate Model Simulations of Snow Accumulation and Firn Compaction

    Science.gov (United States)

    Briggs, K.; Shepherd, A.; Horwath, M.; Horvath, A.; Nagler, T.; Wuite, J.; Muir, A.; Gilbert, L.; Mouginot, J.

    2015-12-01

    Surface mass balance (SMB) estimates from Regional Climate Models (RCMs) are fundamental for assessing and understanding ice sheet mass trends. Mass budget and altimetry assessments rely on RCMs both directly for estimates of the SMB contribution to the total mass trend, and indirectly for ancillary data in the form of firn compaction corrections. As such, mass balance assessments can be highly sensitive to RCM outputs and therefore their accuracy. Here we assess the extent to which geodetic measurements of mass balance are sensitive to RCM model outputs at different resolutions. We achieve this by comparing SMB dependent estimates of mass balance from the mass budget method and altimetry, with those from satellite gravimetry that are independent of SMB estimates. Using the outputs of the RACMO/ANT 2.3 model at 5.5 km and 27 km horizontal spatial resolution, we generate estimates of mass balance using the mass budget method and altimetry for the Western Palmer Land region of the Antarctic Peninsula between 2003 and 2014. We find a 19% increase in the long-term (1980 to 2014) mean annual SMB for the region when enhancing the model resolution to 5.5 km. This translates into an approximate 50% reduction in the total mass loss from 2003 to 2014 calculated with the mass budget method and a 15% increase in the altimetry estimate. The use of the enhanced resolution product leads to consistency between the estimates of mass loss from the altimetry and the mass budget method that is not observed with the coarser resolution product, in which estimates of cumulative mass fall beyond the relative errors. Critically, when using the 5.5 km product, we find excellent agreement, both in pattern and magnitude, with the independent estimate derived from gravimetry. Our results point toward the crucial need for high resolution SMB products from RCMs for mass balance assessments, particularly in regions of high mass turnover and complex terrain as found over the Antarctic Peninsula.

  13. Classification of Satellite Derived Chlorophyll a Space-Time Series by Means of Quantile Regression: An Application to the Adriatic Sea

    Science.gov (United States)

    Girardi, P.; Pastres, R.; Gaetan, C.; Mangin, A.; Taji, M. A.

    2015-12-01

    In this paper, we present the results of a classification of Adriatic waters, based on spatial time series of remotely sensed Chlorophyll type-a. The study was carried out using a clustering procedure combining quantile smoothing and an agglomerative clustering algorithms. The smoothing function includes a seasonal term, thus allowing one to classify areas according to “similar” seasonal evolution, as well as according to “similar” trends. This methodology, which is here applied for the first time to Ocean Colour data, is more robust with respect to other classical methods, as it does not require any assumption on the probability distribution of the data. This approach was applied to the classification of an eleven year long time series, from January 2002 to December 2012, of monthly values of Chlorophyll type-a concentrations covering the whole Adriatic Sea. The data set was made available by ACRI (http://hermes.acri.fr) in the framework of the Glob-Colour Project (http://www.globcolour.info). Data were obtained by calibrating Ocean Colour data provided by different satellite missions, such as MERIS, SeaWiFS and MODIS. The results clearly show the presence of North-South and West-East gradient in the level of Chlorophyll, which is consistent with literature findings. This analysis could provide a sound basis for the identification of “water bodies” and of Chlorophyll type-a thresholds which define their Good Ecological Status, in terms of trophic level, as required by the implementation of the Marine Strategy Framework Directive. The forthcoming availability of Sentinel-3 OLCI data, in continuity of the previous missions, and with perspective of more than a 15-year monitoring system, offers a real opportunity of expansion of our study as a strong support to the implementation of both the EU Marine Strategy Framework Directive and the UNEP-MAP Ecosystem Approach in the Mediterranean.

  14. Comparison of Satellite-Derived TOA Shortwave Clear-Sky Fluxes to Estimates from GCM Simulations Constrained by Satellite Observations of Land Surface Characteristics

    Science.gov (United States)

    Anantharaj, Valentine G.; Nair, Udaysankar S.; Lawrence, Peter; Chase, Thomas N.; Christopher, Sundar; Jones, Thomas

    2010-01-01

    Clear-sky, upwelling shortwave flux at the top of the atmosphere (S(sub TOA raised arrow)), simulated using the atmospheric and land model components of the Community Climate System Model 3 (CCSM3), is compared to corresponding observational estimates from the Clouds and Earth's Radiant Energy System (CERES) sensor. Improvements resulting from the use of land surface albedo derived from Moderate Resolution Imaging Spectroradiometer (MODIS) to constrain the simulations are also examined. Compared to CERES observations, CCSM3 overestimates global, annual averaged S(sub TOA raised arrow) over both land and oceans. However, regionally, CCSM3 overestimates S(sub TOA raised arrow) over some land and ocean areas while underestimating it over other sites. CCSM3 underestimates S(sub TOA raised arrow) over the Saharan and Arabian Deserts and substantial differences exist between CERES observations and CCSM3 over agricultural areas. Over selected sites, after using groundbased observations to remove systematic biases that exist in CCSM computation of S(sub TOA raised arrow), it is found that use of MODIS albedo improves the simulation of S(sub TOA raised arrow). Inability of coarse resolution CCSM3 simulation to resolve spatial heterogeneity of snowfall over high altitude sites such as the Tibetan Plateau causes overestimation of S(sub TOA raised arrow) in these areas. Discrepancies also exist in the simulation of S(sub TOA raised arrow) over ocean areas as CCSM3 does not account for the effect of wind speed on ocean surface albedo. This study shows that the radiative energy budget at the TOA is improved through the use of MODIS albedo in Global Climate Models.

  15. Satellite-derived geoid for the estimation of lithospheric cooling and basal heat flux anomalies over the northern Indian Ocean lithosphere

    Indian Academy of Sciences (India)

    S Rajesh; T J Majumdar

    2015-12-01

    The northern Indian Ocean consists of older Bay of Bengal (BOB) oceanic lithosphere with numerous intra-plate loads; whereas, contrasting elements like active Mid-Ocean ridge divergence and slow spreading ridges are present in the relatively younger (<60 Ma) Arabian Sea oceanic lithosphere. The mechanism of lithospheric cooling of young age oceanic lithosphere from the moderately active and slow spreading Carlsberg Ridge is analysed by considering the hypothesis of near lithospheric convective action or whole upper mantle convection. We addressed these issues by studying the marine geoid at different spatial wavelengths and retrieved and compared their lithospheric cooling signatures, plate spreading and distribution of mass and heat anomalies along with seismicity, bathymetry, gravity and isochron age data. Results show that progressive cooling of young-aged oceanic lithosphere from the Mid-Ocean Carlsberg Ridge is because of conductive cooling and those signals are retrieved in the shorter wavelength band (111 < < 1900 km) of constrained residual geoid with mass anomaly sources near to sublithospheric. This shows steadiness in the geoid anomaly decay rate (∼–0.1 m/Ma), consistency in the growth of thermal boundary layer and progressive fall of basal temperature and heat flux (900–300 K and 100–18 mW m−2) with increase of lithospheric age. The above observations are attributed to the fact that the advective–convective action beneath the Mid-Ocean Carlsberg Ridge is driven by the basal temperature gradient between the lithosphere and the near lithospheric low viscose thin layer. But, for the case of old-aged oceanic lithosphere in the BOB, the residual geoid anomaly cooling signals are not prominently seen in the same band as that of the Arabian Sea because of the Ninetyeast Ridge magmatism. However, its cooling anomaly signatures are retrieved at relatively higher band (1335 ≤ ≤ 3081 km) having erratic geoid decay rates (–0.3 to 0.2 m/Ma) owing

  16. Study of different HILIC, mixed-mode, and other aqueous normal-phase approaches for the liquid chromatography/mass spectrometry-based determination of challenging polar pesticides.

    Science.gov (United States)

    Vass, Andrea; Robles-Molina, José; Pérez-Ortega, Patricia; Gilbert-López, Bienvenida; Dernovics, Mihaly; Molina-Díaz, Antonio; García-Reyes, Juan F

    2016-07-01

    The aim of the study was to evaluate the performance of different chromatographic approaches for the liquid chromatography/mass spectrometry (LC-MS(/MS)) determination of 24 highly polar pesticides. The studied compounds, which are in most cases unsuitable for conventional LC-MS(/MS) multiresidue methods were tested with nine different chromatographic conditions, including two different hydrophilic interaction liquid chromatography (HILIC) columns, two zwitterionic-type mixed-mode columns, three normal-phase columns operated in HILIC-mode (bare silica and two silica-based chemically bonded columns (cyano and amino)), and two standard reversed-phase C18 columns. Different sets of chromatographic parameters in positive (for 17 analytes) and negative ionization modes (for nine analytes) were examined. In order to compare the different approaches, a semi-quantitative classification was proposed, calculated as the percentage of an empirical performance value, which consisted of three main features: (i) capacity factor (k) to characterize analyte separation from the void, (ii) relative response factor, and (iii) peak shape based on analytes' peak width. While no single method was able to provide appropriate detection of all the 24 studied species in a single run, the best suited approach for the compounds ionized in positive mode was based on a UHPLC HILIC column with 1.8 μm particle size, providing appropriate results for 22 out of the 24 species tested. In contrast, the detection of glyphosate and aminomethylphosphonic acid could only be achieved with a zwitterionic-type mixed-mode column, which proved to be suitable only for the pesticides detected in negative ion mode. Finally, the selected approach (UHPLC HILIC) was found to be useful for the determination of multiple pesticides in oranges using HILIC-ESI-MS/MS, with limits of quantitation in the low microgram per kilogram in most cases. Graphical Abstract HILIC improves separation of multiclass polar pesticides.

  17. Normalization in econometrics

    OpenAIRE

    Hamilton, James D.; Daniel F. Waggoner; Zha, Tao

    2004-01-01

    The issue of normalization arises whenever two different values for a vector of unknown parameters imply the identical economic model. A normalization does not just imply a rule for selecting which point, among equivalent ones, to call the maximum likelihood estimator (MLE). It also governs the topography of the set of points that go into a small-sample confidence interval associated with that MLE. A poor normalization can lead to multimodal distributions, disjoint confidence intervals, and v...

  18. Rough endoplasmic reticulum trafficking errors by different classes of mutant dentin sialophosphoprotein (DSPP) cause dominant negative effects in both dentinogenesis imperfecta and dentin dysplasia by entrapping normal DSPP.

    Science.gov (United States)

    von Marschall, Zofia; Mok, Seeun; Phillips, Matthew D; McKnight, Dianalee A; Fisher, Larry W

    2012-06-01

    Families with nonsyndromic dentinogenesis imperfecta (DGI) and the milder, dentin dysplasia (DD), have mutations in one allele of the dentin sialophosphoprotein (DSPP) gene. Because loss of a single Dspp allele in mice (and likely, humans) causes no dental phenotype, the mechanism(s) underling the dominant negative effects were investigated. DSPP mutations occur in three classes. (The first class, the mid-leader missense mutation, Y6D, was not investigated in this report.) All other 5′ mutations of DSPP result in changes/loss in the first three amino acids (isoleucine-proline-valine [IPV]) of mature DSPP or, for the A15V missense mutation, some retention of the hydrophobic leader sequence. All of this second class of mutations caused mutant DSPP to be retained in the rough endoplasmic reticulum (rER) of transfected HEK293 cells. Trafficking out of the rER by coexpressed normal DSPP was reduced in a dose-responsive manner, probably due to formation of Ca2+-dependent complexes with the retained mutant DSPP. IPV-like sequences begin many secreted Ca2+-binding proteins, and changing the third amino acid to the charged aspartate (D) in three other acidic proteins also caused increased rER accumulation. Both the leader-retaining A15V and the long string of hydrophobic amino acids resulting from all known frameshift mutations within the 3′-encoded Ca2+-binding repeat domain (third class of mutations) caused retention by association of the mutant proteins with rER membranes. More 5′ frameshift mutations result in longer mutant hydrophobic domains, but the milder phenotype, DD, probably due to lower effectiveness of the remaining, shorter Ca2+-binding domain in capturing normal DSPP protein within the rER. This study presents evidence of a shared underlying mechanism of capturing of normal DSPP by two different classes of DSPP mutations and offers an explanation for the mild (DD-II) versus severe (DGI-II and III) nonsyndromic dentin phenotypes. Evidence is also

  19. A dose-response strategy reveals differences between normal-weight and obese men in their metabolic and inflammatory responses to a high-fat meal.

    Science.gov (United States)

    Schwander, Flurina; Kopf-Bolanz, Katrin A; Buri, Caroline; Portmann, Reto; Egger, Lotti; Chollet, Magali; McTernan, Philip G; Piya, Milan K; Gijs, Martin A M; Vionnet, Nathalie; Pralong, François; Laederach, Kurt; Vergères, Guy

    2014-10-01

    A dose-response strategy may not only allow investigation of the impact of foods and nutrients on human health but may also reveal differences in the response of individuals to food ingestion based on their metabolic health status. In a randomized crossover study, we challenged 19 normal-weight (BMI: 20-25 kg/m(2)) and 18 obese (BMI: >30 kg/m(2)) men with 500, 1000, and 1500 kcal of a high-fat (HF) meal (60.5% energy from fat). Blood was taken at baseline and up to 6 h postprandially and analyzed for a range of metabolic, inflammatory, and hormonal variables, including plasma glucose, lipids, and C-reactive protein and serum insulin, glucagon-like peptide-1, interleukin-6 (IL-6), and endotoxin. Insulin was the only variable that could differentiate the postprandial response of normal-weight and obese participants at each of the 3 caloric doses. A significant response of the inflammatory marker IL-6 was only observed in the obese group after ingestion of the HF meal containing 1500 kcal [net incremental AUC (iAUC) = 22.9 ± 6.8 pg/mL × 6 h, P = 0.002]. Furthermore, the net iAUC for triglycerides significantly increased from the 1000 to the 1500 kcal meal in the obese group (5.0 ± 0.5 mmol/L × 6 h vs. 6.0 ± 0.5 mmol/L × 6 h; P = 0.015) but not in the normal-weight group (4.3 ± 0.5 mmol/L × 6 h vs. 4.8 ± 0.5 mmol/L × 6 h; P = 0.31). We propose that caloric dose-response studies may contribute to a better understanding of the metabolic impact of food on the human organism. This study was registered at clinicaltrials.gov as NCT01446068. © 2014 American Society for Nutrition.

  20. A Dose-Response Strategy Reveals Differences between Normal-Weight and Obese Men in Their Metabolic and Inflammatory Responses to a High-Fat Meal123

    Science.gov (United States)

    Schwander, Flurina; Kopf-Bolanz, Katrin A.; Buri, Caroline; Portmann, Reto; Egger, Lotti; Chollet, Magali; McTernan, Philip G.; Piya, Milan K.; Gijs, Martin A. M.; Vionnet, Nathalie; Pralong, François; Laederach, Kurt; Vergères, Guy

    2014-01-01

    A dose-response strategy may not only allow investigation of the impact of foods and nutrients on human health but may also reveal differences in the response of individuals to food ingestion based on their metabolic health status. In a randomized crossover study, we challenged 19 normal-weight (BMI: 20–25 kg/m2) and 18 obese (BMI: >30 kg/m2) men with 500, 1000, and 1500 kcal of a high-fat (HF) meal (60.5% energy from fat). Blood was taken at baseline and up to 6 h postprandially and analyzed for a range of metabolic, inflammatory, and hormonal variables, including plasma glucose, lipids, and C-reactive protein and serum insulin, glucagon-like peptide-1, interleukin-6 (IL-6), and endotoxin. Insulin was the only variable that could differentiate the postprandial response of normal-weight and obese participants at each of the 3 caloric doses. A significant response of the inflammatory marker IL-6 was only observed in the obese group after ingestion of the HF meal containing 1500 kcal [net incremental AUC (iAUC) = 22.9 ± 6.8 pg/mL × 6 h, P = 0.002]. Furthermore, the net iAUC for triglycerides significantly increased from the 1000 to the 1500 kcal meal in the obese group (5.0 ± 0.5 mmol/L × 6 h vs. 6.0 ± 0.5 mmol/L × 6 h; P = 0.015) but not in the normal-weight group (4.3 ± 0.5 mmol/L × 6 h vs. 4.8 ± 0.5 mmol/L × 6 h; P = 0.31). We propose that caloric dose-response studies may contribute to a better understanding of the metabolic impact of food on the human organism. This study was registered at clinicaltrials.gov as NCT01446068. PMID:24812072

  1. Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis

    Science.gov (United States)

    Sánchez-Abarca, Luis Ignacio; Rosón-Burgo, Beatriz; Redondo, Alba; Rico, Ana; Preciado, Silvia; Ortega, Rebeca; Rodríguez, Concepción; Muntión, Sandra; Hernández-Hernández, Ángel; De Las Rivas, Javier; González, Marcos; González Porras, José Ramón; del Cañizo, Consuelo; Sánchez-Guijo, Fermín

    2017-01-01

    There is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HD-MSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls. Gene expression profile of BM-MSC showed a total of 169 genes that were differentially expressed in BM-MSC from MPN patients compared to HD-MSC. In addition, we studied the ability of BM-MSC to support the growth and survival of hematopoietic stem/progenitor cells (HSPC), showing a significant increase in the number of CFU-GM colonies when MPN-HSPC were co-cultured with MPN-MSC. Furthermore, MPN-MSC showed alteration in the expression of genes associated to the maintenance of hematopoiesis, with an overexpression of SPP1 and NF-kB, and a downregulation of ANGPT1 and THPO. Our results suggest that BM-MSC from JAK2+ patients differ from their normal counterparts and favor the maintenance of malignant clonal hematopoietic cells. PMID:28796790

  2. Variation in FDG uptakes in different regions in normal human brain as a function of the time (30 and 60 minutes) after injection of FDG

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, Kazunari; Sakamoto, Setsu; Mori, Tetsuya; Sasaki, Masahiro [Hyogo Inst. for Aging Brain and Cognitive Disorders, Himeji (Japan); Hosaka, Kayo [Kobe Univ. (Japan). School of Medicine

    2002-06-01

    The authors' goal was to determine whether FDG uptakes in various regions of the brain are different for early and late scanning time in positron emission tomography (PET). F-18 fluorodeoxyglucose (FDG) PET was performed on 15 healthy normal subjects to obtain early and late acquisition glucose metabolic images (30 and 60 min after FDG injection), respectively. The two sets of images were compared in a voxel-by-voxel analysis. In the bilateral posterior cingulated gyrus, parietal and frontal association cortices, and subcallosal cortices, the FDG uptakes were larger on the late scan image than on the early scan image, and the FDG uptakes were larger in the cerebellar hemisphere, vermis and frontal basis on the early scan image than on the late scan image. These results suggest that there are different regional FDG uptakes depending on the scanning time after FDG injection and we must be careful in replacing conventional FDG PET scanning with early scanning in FDG PET study. (author)

  3. Sensitivity of the Enhanced Vegetation Index (EVI and Normalized Difference Vegetation Index (NDVI to Topographic Effects: A Case Study in High-density Cypress Forest

    Directory of Open Access Journals (Sweden)

    Guoyu Qiu

    2007-11-01

    Full Text Available Vegetation indices play an important role in monitoring variations in vegetation.The Enhanced Vegetation Index (EVI proposed by the MODIS Land Discipline Groupand the Normalized Difference Vegetation Index (NDVI are both global-based vegetationindices aimed at providing consistent spatial and temporal information regarding globalvegetation. However, many environmental factors such as atmospheric conditions and soilbackground may produce errors in these indices. The topographic effect is another veryimportant factor, especially when the indices are used in areas of rough terrain. In thispaper, we theoretically analyzed differences in the topographic effect on the EVI and theNDVI based on a non-Lambertian model and two airborne-based images acquired from amountainous area covered by high-density Japanese cypress plantation were used as a casestudy. The results indicate that the soil adjustment factor “L” in the EVI makes it moresensitive to topographic conditions than is the NDVI. Based on these results, we stronglyrecommend that the topographic effect should be removed in the reflectance data beforethe EVI was calculated—as well as from other vegetation indices that similarly include a term without a band ratio format (e.g., the PVI and SAVI—when these indices are used in the area of rough terrain, where the topographic effect on the vegetation indices having only a band ratio format (e.g., the NDVI can usually be ignored.