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Sample records for sapidus decapoda brachyura

  1. Crustacea, Decapoda, Portunidae, Callinectes sapidus Rathbun, 1896: new record and filling distribution gaps

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    Rosa, Leonardo

    2013-01-01

    The blue crab Callinectes sapidus Rathbun, 1896 (Crustacea: Decapoda: Portunidae) is firstly recorded from the state of Sergipe, NE Brazil, filling a gap in the species distribution along Brazilian coast.

  2. Crustáceos fósiles (Decapoda: Brachyura) de la Formación Colón, Matanzas, Cuba

    OpenAIRE

    Rojas Consuegra, R.; Varela, C.

    2011-01-01

    Three families, four genera and four species of fossil crustacean (Decapoda: Brachyura) were studied. The following taxa are recorded for the first time for the Cuban Oligocene-Miocene belonging to Colon formation, Matanzas, Cuba: Raninoides sp. (Raninidae), Necronectes collinsi Schweitzer et al. (Portunidae), Iliacantha liodactylus Rathbun (Leucosiidae) and Hepatus sp. (Aethridae).

  3. Efectos del rizocéfalo Loxothylacus armatus (Cirripedia: Rhizocephala en el cangrejo Paraxanthus barbiger (Decapoda: Brachyura en Chile Effects of the rhizocephalan Loxothylacus armatus (Cirripedia: Rhizocephala on the crab Paraxanthus barbiger (Decapoda: Brachyura in Chile

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    MARIO GEORGE-NASCIMENTO

    2006-06-01

    Full Text Available Diversas asociaciones biológicas de amplia distribución y alta especificidad en el ambiente marino han pasado casi desapercibidas para los estudiosos en Chile. Aquí se describe cuantitativamente la infección por Loxothylacus armatus Boschma 1949 (Cirripedia: Rhizocephala en el cangrejo Paraxanthus barbiger (Poeppig 1836 (Decapoda: Brachyura y se evalúa si el parásito produce efectos semejantes a los descritos para asociaciones similares. Para ello, 513 ejemplares del cangrejo fueron recolectados manualmente desde el submareal somero, entre septiembre y octubre de 2003, en Lenga, Octava Región de Chile. La prevalencia de rizocéfalos alcanzó valores máximos cercanos al 60 % en hospedadores de pequeño tamaño corporal, provocando castración, inhibiendo el desarrollo gonadal de las hembras y modificando el tamaño de los caracteres sexuales secundarios en ambos sexos, lo que sugiere que impone una gran demanda sobre su hospedador. Estos resultados muestran una gran similitud con otros estudios que involucran a rizocéfalos, y podrían, al incorporar a las simbiosis en el estudio de las comunidades bentónicas en Chile, conducir a que se reinterprete, por ejemplo, la abundancia y patrones de reproducción de P. barbiger, una especie de depredador prominente de esas comunidades bentónicasIn the marine realm, several biological associations are distributed worldwide and are highly specific, but remain poorly studied in Chile. Here, we describe quantitatively the infection by the barnacle Loxothylacus armatus Boschma 1949 (Cirripedia: Rhizocephala in the crab Paraxanthus barbiger (Poeppig 1836 (Decapoda: Brachyura, and assess if the parasite effects are similar to rhizocephalan-decapod associations elsewhere. To do this, 513 crabs were collected by hand while scuba diving between September and October, 2003 in the shallow subtidal zone of Lenga (37° S, Chile. Maximum prevalence reached ca. 60 % in small body size hosts, producing total

  4. Aspectos ecológicos dos Brachyura (Crustacea: Decapoda no manguezal do Itacorubi, SC - Brasil

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    Joaquim Olinto Branco

    1990-01-01

    Full Text Available Ecological studies including occurrence, relative abundance and spatial distribution of 15 species of Brachyura, as well as the fluctuation in the annual cycle are carried out in mangrove (27º 34' 14" and 27º 35' 31" L.S.; 48º 30' 07"and 48º 31' 33" L. W., during the year 1986. The hydrological parameters temperature, salinity and pH of surface and intersticial waters were also studied. Chasmagnathus granulata was the most abundant specie, followed by Uça uruguayensis, Aratus pisonii and Callinectes danae. The number of males was higher than females. In the spring a larger rate of Catches occurred. The species Eurytium limosum, Cardisoma guanhumi that had the state of São Paulo as their austral boundaru and Uça maracoani the state of Paraná, have now their boundaries of distribution expanded is for as the City of Florianópolis, in the state of Santa Catarina.

  5. Seasonality of larvae of Brachyura and Anomura (Crustacea Decapoda in the Beagle Channel, Argentina

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    Gustavo A. Lovrich

    1999-12-01

    Full Text Available This is the first study in the Beagle Channel that reports the larval seasonal distribution, abundance and duration of the larval stages of Anomura and Brachyura, on the basis of a fortnightly sampling programme. An identification key to the specific level of zoeal stages is included. Between September 1987 and March 1989, 304 plankton samples were taken by means of oblique hauls from the bottom to the sea-surface. Eighty-five percent of the samples were taken shallower than 60 m depth. Decapod larvae occurred only in spring and summer. All but two taxa showed a single cohort of larvae which emerge at the beginning of the spring. A second cohort of Munida spp. and Halicarcinus planatus also occurred during summer. The most abundant taxa were Munida spp. (312 larvae ? 10 m-3, Peltarion spinosulum (288 larvae ? 10 m-3,, H. planatus (143 larvae ? 10 m-3, and Pagurus spp. (79 larvae ? 10 m-3, which represented 97% of the total larvae collected. Larvae of Pinnotheridae, Eurypodius latreillii, Libidoclaea granaria, and Paralomis granulosa were about an order of magnitude less abundant. Relative abundances of larvae correspond to relative abundances of the respective benthic stages. The absence of certain larval stages or of certain species (Acanthocyclus albatrossis and Lithodes santolla probably indicates their differential distribution within the Beagle Channel. Particularly, lithodid larvae may have benthic or epibenthic habits.

  6. Brachyura (Crustacea, Decapoda associated to Schizoporella unicornis (Bryozoa, Gymnolaemata in Ubatuba bay (SP, Brazil

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    Fernando L. M. Mantelatto

    1998-01-01

    Full Text Available This work characterizes the composition of Brachyura from Schizoporella unicornis. The samples were collected in 1995 at Itaguá Beach, Ubatuba (SP, at three month intervals, during all seasons from January to December. The Bryozoa colonies were obtained by snorkeling at a depth of five meters in daylight. A total of 323 specimens were collected from four families (Xanthidae, Portunidae, Majidae and Grapsidae. Pachygrapsus transversus, Hexapanopeus schimitti, and Menippe nodifrons occurred in all seasons. The highest and lowest number of individuals occurred during the spring and autumn, respectively. Xanthidae exhibited the highest density during the summer, autumn, and spring, while Grapsidae exhibited the highest density in winter. It was noted the presence of Charibdis hellerii, an portunid from Indo-Pacific ocean. The diversity of species obtained, in addition to an accentuated number of immature and ovigerous females specimens, suggested that Schizoporella colonies were a place of reproduction and development.Este trabalho caracterizou a composição dos braquiúros em Schizoporella unicornis. As amostras foram coletadas na Praia do Itaguá, Ubatuba (SP, em intervalos de três meses, durante as estações climáticas de Janeiro a Dezembro/1995. As colônias de briozoários foram obtidas por mergulho livre, coletadas pela manhã. Um total de 323 espécimes foram coletados dentro de quatro famílias (Xanthidae, Portunidae, Majidae e Grapsidae. Pachygrapsus transversus, Hexapanopeus schimitti e Menippe nodifrons ocorreram em todas as estações. O maior e o menor número de indivíduos foram registrados na primavera e no outono, respectivamente. Xanthidae exibiu maior densidade no verão, outono e primavera, enquanto Grapsidae foi no inverno. Registrou-se a ocorrência de Charibdis hellerii, espécie originária do Indo-Pacífico. A diversidade de espécies obtida, juntamente com o acentuado número de indivíduos imaturos e fêmeas ov

  7. Histological Description of Gonadal Development of Females and Males of Callinectes sapidus Rathbun, 1896 (Decapoda: Portunidae).

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    Carvalho-Saucedo, Liliana; Ramírez-Santiago, Cecilia; Pérez, Carlos

    2015-04-01

    This paper describes the characteristics of male and female germ cells during gonadal development and the gonadal maturity scale of the blue crab, Callinectes sapidus. A total of 20 specimens were collected monthly from June to November 2012, in two areas off the coast of the Gulf of Mexico: the San Andrés Lagoon and Alvarado Lagoon. The gonads were removed and processed following the standard technique of hematoxylin and eosin staining. An important event in oogenesis (pre-vitellogenesis) was the appearance of a perinuclear vesicle in the cytoplasm and the accumulation of yolk granules. Later, vitellogenesis began and there was an accumulation of nutritive droplets and fragmentation of the perinuclear vesicle. During spermatogenesis, the accumulation of two fluids was observed that were involved in the formation of the spermatophore and the delay of spermiogenesis. Based on the histological features of gonad maturity, five stages were described (inactive, early gametogenesis, development, maturity, and resorption), in females and males. This proposal can be useful for the study of reproductive seasonality of this species.

  8. Significance of the sexual openings and supplementary structures on the phylogeny of brachyuran crabs (Crustacea, Decapoda, Brachyura), with new nomina for higher-ranked podotreme taxa.

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    Guinot, Danièle; Tavares, Marcos; Castro, Peter

    2013-01-01

    it is lost in all other raninoid extant members. New evidence shows that the abdominal holding was an early occurrence for a brachyuran crab. The Raninoidea, sister to Palaeocorystoidea, is characterised by gymnopleurity, a condition that results from the lifting of the carapace and thus the exposure of several pleurites. The narrowing of the body and thoracic sternum, almost certainly associated with their burrowing behaviour, is a diagnostic feature of raninoid evolution, in contrast to the widening observed in the remaining Brachyura. The monophyly of Heterotremata is discussed. Although the correct assignment of the coxal male gonopore and sternal female gonopore (vulva) at the base of Decapoda and Eubrachyura, respectively, left no synapomorphies to support the Heterotremata, the group nevertheless should be regarded as the sister group to Thoracotremata. The controversial monophyly of Podotremata is discussed and arguments are presented against the suppression of this taxon. The distinction of Homoloidia from Dromioidia is argued, and a classification of Podotremata, which considers the fossil record whenever possible, is presented. The earliest brachyurans are re-examined, and a new interpretation of the phylogeny of several basal eubrachyuran groups (Dorippoidea, Inachoididae, Palicoidea, Retroplumoidea) is proposed. Stenorhynchus shares a number of characters with the Inachoididae that differentiate them from Inachidae, and also has some distinctive features that warrants its assignment to a separate inachoidid subfamily, Stenorhynchinae, which is resurrected. The concealment strategies among Brachyura are documented and discussed. Podotremes use carrying behaviour, often combined with burying and concealment under substrates, whereas living within a host, burying, and decoration are used by heterotremes, burrowing being essentially a thoracotreme strategy. (ABSTRACT TRUNCATED)

  9. DECAPODA, BRACHYURA, MAJIDAE

    African Journals Online (AJOL)

    mass, with longitudinal raised and rounded median area; first segment narrowly rectangular; second segment slightly wider, with lateral tubercle; segments three to five similar to second segment but becoming progressively longer; sixth segment ...

  10. Morphology of the first zoeal stages of five species of the portunid genus Callinectes (Decapoda, Brachyura) hatched at the laboratory.

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    Mantelatto, Fernando L; Reigada, Alvaro L D; Gatti, Aline C R; Cuesta, José A

    2014-05-23

    The genus Callinectes Stimpson, 1860 currently consists of 16 species, six of which are reported in Brazilian coast. In the present study, the first zoeal stages of Callinectes bocourti, C. danae, C. exasperatus, C. ornatus and C. sapidus from Brazil were obtained from ovigerous females. The morphological and meristic characters of all these larval stages are described and illustrated. Those of C. bocourti, C. danae and C. sapidus are redescribed and compared with the previous descriptions, and differences are listed. Larval characters of these species were examined for interspecific differences, as well as larval features to distinguish the genus Callinectes within Portunidae. In addition, other portunid genera and species with a known first zoeal stage are compared, with special attention to those species present in the same geographical area. Our findings concord with some previous molecular studies, and we discuss the complexity within the group.

  11. Morphology of the first zoeal stages of five species of the portunid genus Callinectes (Decapoda, Brachyura hatched at the laboratory

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    FERNANDO L. MANTELATTO

    2014-06-01

    Full Text Available The genus Callinectes Stimpson, 1860 currently consists of 16 species, six of which are reported in Brazilian coast. In the present study, the first zoeal stages of Callinectes bocourti,C. danae, C. exasperatus, C. ornatus and C. sapidus from Brazil were obtained from ovigerous females. The morphological and meristic characters of all these larval stages are described and illustrated. Those of C. bocourti, C. danae and C. sapidus are redescribed and compared with the previous descriptions, and differences are listed. Larval characters of these species were examined for interspecific differences, as well as larval features to distinguish the genus Callinectes within Portunidae. In addition, other portunid genera and species with a known first zoeal stage are compared, with special attention to those species present in the same geographical area. Our findings concord with some previous molecular studies, and we discuss the complexity within the group.

  12. Distribución espacial y temporal del cangrejo Callinectes sapidus (Decapoda: Portunidae en la Bahía de Chetumal, Quintana Roo, México

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    Héctor J Ortiz-León

    2007-03-01

    sapidus (Decapoda: Portunidae in Chetumal Bay, Quintana Roo, Mexico. In order to determine temporal and spatial distribution patterns of Callinectes sapidus, samplings were carried out during the cold-front (January-February, dry (May-June and rainy (August-September, 2002 climatic seasons, in 30 sampling stations of Chetumal Bay, grouped in sectors A (14 stations, B (eight stations and C (eight stations. In each sampling station crabs were collected from two transects parallel to the coast, each with three traps, separated by 30 m. Sediments were calcareous coarse and medium sand, white or lightly gray. A total of 1 031 specimens were collected. CPEU (Capture Per Effort Unit differed spatially and temporally. Highest CPEU was found in sector C with 1.3 ind∙trap-1, and in the rainy season with 1.1 ind∙trap-1. Population was predominantly composed of male individuals. The male:female ratio was 15:1. Males and adults (group II CPEU was significant different between sectors and climatic seasons. Both males and adults (group II had a greater CPEU in sector C (1.2 ind∙trap-1 and in the rainy season (1.1 ind∙trap-1. Abundance of female and juvenile individuals (group I was low during the sampling period whereas group 0 juvenile individuals were not found. A greater relative frequency between sectors and climatic seasons were observed in 130-139 mm and 140-149 mm size interval (CW. C. sapidus occurred on sandy sediments in Chetumal Bay. Pearson product moment correlations exhibited significant relationships between CPEU and temperature, salinity and dissolved oxygen. In Chetumal Bay, the spatial and temporal distribution of C. sapidus can be related to salinity, temperature, habitat quality, food availability, recruitment and reproduction events of individuals. Rev. Biol. Trop. 55 (1: 235-245. Epub 2007 March. 31.

  13. Fecundity, reproductive seasonality and maturation size of Callinectes sapidus females (Decapoda: Portunidae) in the Southeast coast of Brazil.

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    Severino-Rodrigues, Evandro; Musiello-Fernandes, Joelson; Moura, Alvaro A S; Branco, Geisa M P; Canéo, Victor O C

    2013-06-01

    C sapidus fisheries has a significant influence on the economy of some countries in North America and has a relative extensive literature in these regions. However, only few papers discuss the ecology of C. sapidus in the South Atlantic, despite its economic importance in that region. We studied the fecundity, reproductive seasonality and maturation size of C. sapidus females captured in the Southeast coast of Brazil from January to December 2002. Females were separated, weighted (Wt), and cephalothorax width (CW) was measured. Furthermore, the eggs-masses were classified according to embryonic development, separated, weighted (We) and fixed. Eggs were also separated and counted, resulting in the average number of eggs per individual (Ne). A total sample of 307 females was collected: 78 young, 130 adults and 99 ovigerous. Ovigerous females showed CW between 7.49 and 15.89cm with average of 12.21cm and were distributed throughout the sample period, with highest incidence between December and March. The onset of morphological maturity (L50) occurred at CW = 10.33 cm, and the size in which all were mature (L100) was CW = 11.20 cm. Individual fecundity ranged from 689 356 to 3 438 122 with an average of 2 006 974. The CW showed a positive growth trend with Ne and We. We concluded that in order to ensure the resource sustainability, it is necessary to prohibit captures of C. sapidus in these regions, especially during summer. Additionally, our studies suggest that the minimum capture size should be 11 cm of carapace width.

  14. Fecundity, reproductive seasonality and maturation size of Callinectes sapidus females (Decapoda: Portunidae in the Southeast coast of Brazil

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    Evandro Severino-Rodrigues

    2013-06-01

    Full Text Available C. sapidus fisheries has a significant influence on the economy of some countries in North America and has a relative extensive literature in these regions. However, only few papers discuss the ecology of C. sapidus in the South Atlantic, despite its economic importance in that region. We studied the fecundity, reproductive seasonality and maturation size of C. sapidus females captured in the Southeast coast of Brazil from January to December 2002. Females were separated, weighted (Wt, and cephalothorax width (CW was measured. Furthermore, the eggs-masses were classified according to embryonic development, separated, weighted (We and fixed. Eggs were also separated and counted, resulting in the average number of eggs per individual (Ne. A total sample of 307 females was collected: 78 young, 130 adults and 99 ovigerous. Ovigerous females showed CW between 7.49 and 15.89cm with average of 12.21cm and were distributed throughout the sample period, with highest incidence between December and March. The onset of morphological maturity (L50 occurred at CW=10.33cm, and the size in which all were mature (L100 was CW=11.20cm. Individual fecundity ranged from 689 356 to 3 438 122 with an average of 2 006 974. The CW showed a positive growth trend with Ne and We. We concluded that in order to ensure the resource sustainability, it is necessary to prohibit captures of C. sapidus in these regions, especially during summer. Additionally, our studies suggest that the minimum capture size should be 11cm of carapace width.

  15. Fecundity, reproductive seasonality and maturation size of Callinectes sapidus females (Decapoda: Portunidae in the Southeast coast of Brazil

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    Evandro Severino-Rodrigues

    2013-06-01

    Full Text Available C. sapidus fisheries has a significant influence on the economy of some countries in North America and has a relative extensive literature in these regions. However, only few papers discuss the ecology of C. sapidus in the South Atlantic, despite its economic importance in that region. We studied the fecundity, reproductive seasonality and maturation size of C. sapidus females captured in the Southeast coast of Brazil from January to December 2002. Females were separated, weighted (Wt, and cephalothorax width (CW was measured. Furthermore, the eggs-masses were classified according to embryonic development, separated, weighted (We and fixed. Eggs were also separated and counted, resulting in the average number of eggs per individual (Ne. A total sample of 307 females was collected: 78 young, 130 adults and 99 ovigerous. Ovigerous females showed CW between 7.49 and 15.89cm with average of 12.21cm and were distributed throughout the sample period, with highest incidence between December and March. The onset of morphological maturity (L50 occurred at CW=10.33cm, and the size in which all were mature (L100 was CW=11.20cm. Individual fecundity ranged from 689 356 to 3 438 122 with an average of 2 006 974. The CW showed a positive growth trend with Ne and We. We concluded that in order to ensure the resource sustainability, it is necessary to prohibit captures of C. sapidus in these regions, especially during summer. Additionally, our studies suggest that the minimum capture size should be 11cm of carapace width.Hay pocos estudios sobre la ecología de C. sapidus en el Atlántico Sur, a pesar de su importancia económica en la región. El presente estudio tiene como objetivo conocer la fecundidad, periodo reproductivo y talla de primera madurez morfológica de las hembras de C. sapidus, con el fin de contribuir a una mejor comprensión de la especie en el Atlántico occidental, así como ayudar en la toma de medidas de gestión en la región. Las

  16. Crustáceos no cerrito Ariano Souza, Rio Grande, Rio Grande do Sul e distribuição de Callinectes sapidus (Brachyura, Portunidae Crustaceans in the archaeological site Ariano Souza, Rio Grande, Rio Grande do Sul and distribution of Callinectes sapidus (Brachyura, Portunidae

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    Cléverson Ranniéri M. dos Santos

    2004-03-01

    Full Text Available While all species of the genus Callinectes Stimpson, 1860 have a continuous distribution on the Atlantic coast of the Americas, Callinectes sapidus Rathbun, 1896 is the only one with disjunct distribution. Considering that this species was introduced in Europe and Japan, it has been suggested that the occurrence of C. sapidus on the southern coast of Brazil was due to the transport by ballast water. In the archaeological site Ariano Souza, located in the estuary of the Patos Lagoon (southern Brazil, remains of crustaceans, including claws of approximately two thousand years ago, were found. A preliminary analysis of this material showed Callinectes chelae. Because this archaeological site is located inside the estuary, it has been hypothesized that these chelae belong either to C. sapidus or to C. danae Smith, 1869. A comparison between pincers collected in the archaeological and pincers of these two species (90 dactyls, 30 of each type was performed. The analysis (ANOVA considered the variability of seven characters of the dactyls, and demonstrated the existence of two groups. Results showed that the measured characters suffice to separate these species, and indicated that the material found in the archaeological site belongs to C. sapidus. The hypothesis of the introduction of C. sapidus in the area is rejected. The possible biogeographic history of the species is discussed.

  17. Crescimento de Callinectes sapidus (Crustacea, Decapoda, Portunidae no estuário da laguna dos Patos, RS, Brasil

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    Leonardo S. Ferreira

    2008-03-01

    Full Text Available Utilizando o método de deslocamento modal para a identificação das idades, estimou-se o crescimento do siri-azul Callinectes sapidus Rathbun, 1896 em duas áreas de pesca no estuário da Lagoa dos Patos. Os indivíduos foram coletados entre fevereiro de 2005 e março de 2006 no Saco da Mangueira e Saco do Arraial por meio de arrasto de rede de portas da pesca artesanal. Coletou-se um total de 2.609 animais, sendo 1.193 machos e 1.416 fêmeas. Para obtenção das curvas de crescimento utilizou-se o modelo de von Bertalanffy. As curvas foram validadas pela sua adequação ao ciclo de vida e aspectos biológicos da espécie. O tamanho máximo de largura de carapaça (LCmáx utilizado foi mantido fixo em todas as análises (LCmáx=162,71mm; ± d.p.=3,10 para machos e LCmáx=157,78mm; ± d.p.=5,45 para fêmeas, sendo esses valores médios das maiores medidas obtidas em mais de 20 anos de coletas no estuário. Os parâmetros de crescimento e longevidade foram estimados para machos (Saco da Mangueira, K=0,0039/dia; t o=-6.07; 1.195 dias; Saco do Arraial, K=0,0041/dia; t o=-5,84; 1.102 dias e fêmeas (Saco da Mangueira, K=0,0040/dia; t o=-6,22; 1.153 dias; Saco do Arraial, K=0,0039/dia; t o=-5.91; 1.181 dias. As curvas de crescimento estimadas nesse trabalho denotam que a espécie atinge o tamanho mínimo de captura praticamente no primeiro ano de vida (120mm.

  18. Composição e distribuição dos Crustácea (Decapoda na Lagoa do Peixe, Rio Grande do Sul, Brasil Composition and distribution of Crustácea (Decapoda from Lagoa do Peixe, Rio Grande do Sul, Brazil

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    Sandro Santos

    2000-03-01

    Full Text Available The Lagoa do Peixe ("Peixe's Lagoon" is located between Atlantic Ocean and Lagoa dos Patos and is 30 Km long and 0,5-2,0 Km wide (31º13'S, 50º55'W -31º26'S, 51º09'. For one year, the composition and distribution of Crustácea Decapoda were analyzed monthly (from July/1994 to June/1995, in seven subareas (terrestrial border and aquatic area and physical and chemical parameters (depth, salinity, sediment texture, oxygen in water, organic material of sediment and water temperature were registered. Decapoda from the families Grapsidae, Ocypodidae, Portunidae (Brachyura, Diogenidae (Anomura and Penaeidae (Penaeidea were captured. Chasmagnathus granulata Dana 1851 was found ali months near the canal connecting Atlantic Ocean and Lagoa do Peixe, but inside the lagoon it was collected only in October/94 and June/95, and in extreme subareas it was sampled in February/95 when the salinity was 31 ‰ at these sites. Callinectes sapidus Rathbun, 1896 and Cyrtograpsus angulatus Dana, 1851 were captured throughout the year, except October/94 and April/95, respectively. Both species were found in ali áreas of sample, indicating a high plasticity in relation to the factors analyzed in this study. Another species captured probably come in the lagoon in specific months, when the environment provides ideal conditions for their develo-pments.

  19. Illustrated Checklist of the Decapoda at Europa Island

    African Journals Online (AJOL)

    Davie PJF (2002) Crustacea: Malacostraca: Eucarida (Part 2): Decapoda - Anomura,. Brachyura. In: Wells, A., Houston,. W. W. K. (eds) Zoological Catalogue of Australia. CSIRO Publishing,. Melbourne, 641 pp. De Grave S, Fransen CH (2011) Carideorum. Catalogus: The Recent Species of the. Dendrobranchiate,.

  20. Names and publication dates of the Brachyura in F.É. Guérin (Guérin-Méneville) (Crustacea: Decapoda).

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    Low, Martyn E Y; Ng, Peter K L; Evenhuis, Neal L

    2013-11-12

    The names and dates of the publications of Brachyura of Guérin (Guérin-Méneville from 1836) are reviewed, and previously unidentified or overlooked names are identified. Several identical new names used in multiple publications by Guérin (also under the name Guérin-Méneville), and others that appeared in the same year necessitated the accurate determination of publication dates to establish priority. The authorships of three names should be credited to Guérin (1832): Gecarcinus lateralis (Gecarcinidae), Halimus aries (Majidae), and Libinia spinosa (Epialtidae), the first previously attributed to Fréminville (1835), the last two to H. Milne Edwards (1834). The overlooked genus- and species-group names Cyclocarcinus pinnotheroides Guérin-Méneville, 1838, are determined to be senior subjective synonyms of the genus- and species-group names Hapalonotus reticulatus (De Man, 1879) (Pilumnidae). Applying Article 23.9.1 of the International Code of Zoological Nomenclature, precedence is reversed between the genus-group names and the correct name is Hapalonotus pinnotheroides (Guérin-Méneville, 1838). Current and widespread use of the genus-group name Thalamita Latreille, 1829 (31 March) (Portunidae) is threatened by the overlooked Thalamites Guérin, 1829 (21 March), and the precedence of the names is also reversed to maintain usage of the former. The genus-group name Eurypodius Guérin (Inachidae) is shown to have been established in 1828, not 1825. Included is a complete bibliography of the publications in which Guérin (also under Guérin-Méneville) established new names for Brachyura, with their accurate publication dates.

  1. [Evaluation of the growth of Callinectes sapidus (Decapoda: Portunidae) by the use of lengthbased methods based on size in Tamaulipas, Mexico].

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    Rodríguez-Castro, Jorge Homero; Ramírez, José A; Velázquez-de-la-Cruz, Gonzalo; Correa-Sandoval, Alfonso

    2016-06-01

    The capture blue crab (Callinectes sapidus) is one of the major fisheries of the state of Tamaulipas, Mexico; both in volume and selling price, as well as employment generation, but there is little information on its biological characteristics. The aim of this study was to evaluate the growth parameters of the blue crab, establishing the most appropriate method. We estimated the length frequency of 17 814 crabs from commercial catch of thirteen locations, including four coastal lagoons. The lagoons were El Barril, Madre, Morales and San Andrés from Tamaulipas, State. Growth parameters were evaluated using indirect methods ELEFAN, PROJMAT and SLCA in combination with the jackknife technique to establish the uncertainty of estimates inherent in each method. The growth parameters L∞ and k were consolidated for purposes of comparison with the growth index phi prime (Φ’). With a mode of 110 mm, the interval carapace length varied between 60 and 205 mm. The values of the growth parameters varied according to the method used. Using SLCA, L∞ varied between 259 and 260 mm and k ranged between 0.749 and 0.750 /year; with PROJMAT, L∞ recorded values between 205 and 260 mm, k fluctuated between 0.550 and 0.740/year, and with ELEFAN, L∞ ranged between 156 and 215 mm and k varied between 0.479 and 0.848/year. Estimates by jackknife detected no variability in Φ’ between locations and significant differences between methods. The ranges of values of Φ’ and PROJMAT estimated SLCA (4.70 to 4.71 and 4.66 to 4.70, respectively) were in the range reported in the literature (4.201-4.798), while lower values ELEFAN contributed significantly (3.87 to 4.27). The SLCA and PROJMAT methods in combination with the jackknife technique, proved to be the most suitable to estimate the growth parameters of C. sapidus.

  2. Potamonautid river crabs (Decapoda, Brachyura, Potamonautidae ...

    African Journals Online (AJOL)

    EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · http://dx.doi.org/10.4314/wsa.v27i1.5015 · AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use ...

  3. Comparação do crescimento entre Callinectes sapidus (Crustacea, Decapoda, Portunidae coletados em campo e mantidos em condições contoladas Growth comparison between Callinectes sapidus (Crustacea, Decapoda, Portunidae collected on the field and maintained under controlled conditions

    Directory of Open Access Journals (Sweden)

    Marcos A. Rodrigues

    2008-09-01

    Full Text Available Devido à falta de estruturas rígidas para determinação de idade em crustáceos, métodos que se baseiam na determinação de idade através da distribuição da freqüência de comprimentos são comumente utilizados. Neste trabalho, foi comparado o crescimento do siri-azul, Callinectes sapidus Rathbun, 1896, em laboratório e a partir de indivíduos de campo. Os indivíduos de laboratório, após a coleta, tiveram seu crescimento acompanhado individualmente. A temperatura foi mantida constante (25±2°C e a salinidade a 20±5. Os indivíduos do campo foram coletados entre 2002 e 2004 em pontos pré-determinados no estuário da Laguna dos Patos, com rede de portas. A biometria, nos dois casos, constou da medida da largura da carapaça (LC mm. As estimativas das curvas de crescimento, segundo o modelo de Bertalanffy para os indivíduos de campo, foram obtidas pelo acompanhamento do deslocamento modal (MPA. O tamanho máximo de largura de carapaça utilizado foi mantido fixo em todas as análises (157,78 mm para fêmeas e 162,71 mm para machos. Para os indivíduos mantidos em laboratório, os parâmetros e a longevidade obtidos foram k=0,001/dia; t o=-1,53; longevidade de 3117 dias (8,5 anos para machos e k=0,002/dia; t o=-29,5; longevidade de 2795 dias para fêmeas (7,7 anos. Os parâmetros de crescimento e a longevidade estimados para os animais coletados em campo foram k=0,004/dia; t o=-4,23; 1267 dias (3,5 anos para machos e k=0,004/dia; t o=-3,71; 1260 dias (3,45 anos para fêmeas. Foram encontradas diferenças significativas entre animais de laboratório e de campo, sugerindo que a espécie responde de forma diferente ao ambiente em que está inserida.The absence of hard structures which register age on crustaceans requires the use of length-based methods for age determination, and modal progression analysis (MPA to estimate growth. In this paper, individual growth of the blue crab Callinectes sapidus Rathbun, 1896 was studied in the

  4. Dieta natural do siri-azul Callinectes sapidus (Decapoda, Portunidae na região estuarina da Lagoa dos Patos, Rio Grande, Rio Grande do Sul, Brasil Natural diet of the blue crab Callinectes sapidus (Decapoda, Portunidae in the Patos Lagoon estuary area, Rio Grande, Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Alexandre Oliveir

    Full Text Available Na região estuarina da Lagoa dos Patos, o siri-azul Callinectes sapidus Rathbun, 1869 é o mais abundante dentre as espécies do gênero. Apesar do siri-azul exercer influência sobre as comunidades bentônicas, por ser considerado um predador do topo da cadeia alimentar, não existem muitos estudos sobre aspectos ecológicos, inclusive sobre a composição e variabilidade sazonal de sua dieta natural, neste estuário. O objetivo principal deste estudo foi investigar a composição da dieta do siri-azul, evidenciando possíveis relações com a comunidade bentônica da região estuarina da Lagoa dos Patos. O período de estudo foi de março de 2003 a março de 2004, com coletas bimestrais. Os organismos foram coletados com auxílio de rede de arrasto de fundo, com malha de 13 mm entre nós opostos. Ainda em campo os animais foram fixados em formol 10%. Após a coleta os animais foram separados quanto ao sexo, medidos (largura e comprimento da carapaça - cm e pesados (peso - g. Após a triagem, os animais foram dissecados e os intestinos retirados e pesados. As análises dos conteúdos alimentares dos siris demonstraram que os hábitos alimentares são diversificados, constituindo-se principalmente de invertebrados bentônicos. O item encontrado com maior freqüência foi Detrito, seguido pelo molusco filtrador Erodona mactroides Bosc, 1802 (Erodonidae. Crustáceos da classe Ostracoda e grãos de areia foram importantes componentes dos conteúdos dos intestinos anteriores, sendo que areia não foi considerada como item alimentar, propriamente dito. Também foram encontradas cerdas e mandíbulas de poliquetos, além de sementes das macrófitas Ruppia maritima L. (Potamogatonaceae e Zannichellia palustris L. (Potamogatonaceae. Este estudo serve como subsídio para medidas de proteção e conservação da população do siri-azul, bem como caracteriza relações tróficas com comunidades bentônicas do estuário da Lagoa dos Patos.The Southern

  5. Modelagem de ocorrência e densidade de juvenis de Callinectes sapidus (Decapoda, Portunidae em dois estuários do Rio Grande do Sul, com dados zero-inflacionados

    Directory of Open Access Journals (Sweden)

    Ana Carolina Braga Martins

    2014-06-01

    Full Text Available Dados de contagem de juvenis de siri-azul (Callinectes sapidus Rathbun, 1896 coletados em dois estuários do Rio Grande do Sul são objeto do presente estudo. Por se encontrarem zero-inflacionados, esses dados motivaram a formulação de modelos hierárquicos, que quantificam o efeito das covariáveis categóricas mês e local sobre a probabilidade de ocorrência e densidade dessas populações, levando em conta a detecção imperfeita. Foram também desenvolvidos modelos não-hierárquicos para comparação. Uma abordagem Bayesiana foi adotada para a estimação dos parâmetros dos modelos por simulação Monte Carlo com Cadeias de Markov (MCMC. A comparação entre modelos foi feita com o Critério de Informação da Deviância (DIC. Os modelos hierárquicos apresentaram ajustes melhores que os modelos convencionais, mitigaram o problema do excesso de zeros e permitiram analisar simultaneamente as probabilidades de ocorrência e a densidade de juvenis de siri-azul. No estuário da Lagoa dos Patos, a probabilidade de ocorrência de juvenis na Classe 2 aumenta com a distância da desembocadura, enquanto em Tramandaí os pontos intermediários apresentam as maiores probabilidades. Em ambos os estuários a ocorrência é mais provável nos meses de verão e de inverno. A densidade de juvenis da Classe 2 apresenta marcada variação em relação aos meses do ano sendo, em geral, maior no estuário de Tramandaí.

  6. Evaluation and management of blue crab Callinectes sapidus (Rathbun, 1896 (Decapoda - Portunidae fishery in the Estuary of Cananéia, Iguape and Ilha Comprida, São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    JT. Mendonça

    Full Text Available The Estuary of Cananéia, Iguape and Ilha Comprida is a part of the Estuarine Lagoon System of Cananéia, Iguape and Paranaguá , on the south coast of São Paulo State, Brazil. It has been recognised for its high standards of environmental conservation. The objective of this paper was to assess the blue crab (Callinectes sapidus fishing in the region, from January 1998 to December 2006, and obtain supportive data to implement technical rules for the rational exploitation and resulting conservation of this resource. The results show a remarkable decrease in the fisheries' abundance index (CPUE up to 2005, from 9.4 to 4.0 kg/hour, mainly caused by an increase in fishing effort, from 1,960 to 13,776 hours on a yearly basis. With the decrease in the fishing effort in 2005 and 2006, the abundance index reacted positively, indicating a recovery of the exploited population. Due to the lack of appropriate regulation limiting the fishing effort and organising the fluctuating admission rates of new fishermen, there is a noticeable increase of the effort, above the sustainable level. Therefore, there is a need for measures to limit and control the admission of fishermen in this activity. One of the measures to be considered is the establishment of regular periods where fishing the species is prohibited (called "defeso", each year, especially from September to January. Another measure is to create a fishermen registry and special licenses, to limit the number of new entrants. Such actions require, for their implementation, a co-managed initiative, involving the blue crab fishermen themselves, governmental agencies and resource management researchers, and the integration of ecological, socioeconomic and cultural dimensions.

  7. New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea

    Directory of Open Access Journals (Sweden)

    Fernando L. Mantelatto

    Full Text Available Abstract We designed 14 new primers for amplification of the COI barcode region of decapod crustacean species. We tested, with high level of success, the generation of ~ 640 ± 49 base-pair sequences in selected groups of decapods (hermit crabs, squat lobsters, marine and freshwater crabs and shrimps, encompassing representatives of 27 genera of 15 families, 11 of Pleocyemata (Anomura, Brachyura, and Caridea and 4 of Dendrobranchiata. Based on the results we expect the applicability of these primers for several studies with different taxa within Decapoda.

  8. Tidal exchange of larvae of Sesarma catenata (Decapoda, Brachyura)

    African Journals Online (AJOL)

    1992-10-02

    Oct 2, 1992 ... bimonthly during spring and neap tides from October to March at the tidal inlet. Samples were collected hourly for 25 h in February, and for 13 h in all other months. Hourly estimates of water flux through the tidal inlet of the estuary were calculated using a generalized one-dimensional hydrodynamic model ...

  9. (Callinectes sapidus) from Contaminated Site

    African Journals Online (AJOL)

    To elucidate seasonal changes in hydrocarbons including polycyclic aromatic hydrocarbons (PAHs) due to oil spill, tissues of blue crab (Callinectes sapidus) were investigated. Total petroleum hydrocarbons (n-alkanes) concentrations ranged from 0.17-0.32ìg/gdw and 0.28-0.62ìg/gdw during the dry and wet seasons ...

  10. Palicus Alternatus Rathbun (Decapoda: Brachyura un nuevo registro para el Caribe colombiano Palicus Alternatus Rathbun (Decapoda: Brachyura un nuevo registro para el Caribe colombiano

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    Campos Néstor Hernando

    1991-06-01

    Full Text Available Se describe por primera vez para el Caribe colombiano un representante de la familia de cangrejos Palicidae, Palicus alternatus Rathbun, 1897, de la región de Santa Marta. Se complementa la descripción y se hacen anotaciones sobre su dimorfismo sexual. One member of the crab family Palicidae, Palicus alternatus Rathbun, 1897, is described by the first time for the Colombian Caribbean; the species was collected in the Santa Marta region. The description of P. alternatus is completed, including a discussion of its sexual dimorphism.

  11. Sistema reproductivo de hembras y machos en Cancer setosus (Molina, 1782 (Decapoda, Brachyura The reproductive system of females and males in Cancer setosus (Molina, 1782 (Decapoda, Brachyura

    Directory of Open Access Journals (Sweden)

    Merari Goldstein

    2010-01-01

    Full Text Available Las hembras del género Cáncer presentan una espermateca cuya conexión con el ovario puede ser dorsal o ventral, dependiendo de la especie. La espermateca es una estructura especializada para la recepción de los espermatóforos los cuales son colocados por el macho durante la cópula. El objetivo de este estudio es la descripción y medición de las estructuras reproductivas de ambos sexos en la jaiba peluda Cáncer setosus. Las hembras presentan un par de ovarios de conformación tipo H, siendo los lóbulos anteriores 2,4 veces más largos que los lóbulos posteriores. La espermateca es de paredes no extensibles y presentó una conexión de tipo ventral con el oviducto, lo que indica una fecundación interna para esta especie. El 64% de las hembras estudiadas presentó un par de tapones espermáticos: En los machos el primer par de pleópodos es tubular con su extremos distal aguzado y/o abierto formando un doblez. El segundo par de pleópodos termina en una estructura en forma de embudo con 12 prolongaciones laterales. Los testículos pares tienen un color blanquecino, y convergen en un vaso deferente, en el cual se puede distinguir tres zonas: una proximal, media y posterior. Cada uno de estos tiene un rol en la elaboración de los espermatóforos y la producción de líquido seminal.Females of the genus Cancer have a spermathecae whose connection with the ovary can be dorsal or ventral, depending on the species. The spermathecae is a structure specialized in receiving the spermatophores released by the male during mating. This study aims to describe and measure the reproductive structures of both sexes in the crab C setosus. Females have a pair of ovaries in an H-type conformation; the anterior lobes are 2.4 times longer than the posterior lobes. The spermathecae has walls that are not extensible and a ventral-type connection with the oviduct, which indicates internal fertilization for this species. Sixty-four percent (64% of the studied females presented a couple of spermatic blockages. In males, the first pair of pleopods is tubular, with the distal tips pointed and/or open, forming a fold. The second pair of pleopods ends in a funnel structure with 12 side extensions. The two testes are whitish in color and converge in a vas deference (VD that has three zones: proximal, middle, and posterior. Each of these plays a role in the elaboration of the spermatophores and seminal fluid production.

  12. The role of alternate hosts in the ecology and life history of Hematodinium sp., a parasitic dinoflagellate of the blue crab (Callinectes sapidus).

    Science.gov (United States)

    Lohan, Katrina M Pagenkopp; Reece, Kimberly S; Miller, Terrence L; Wheeler, Kersten N; Small, Hamish J; Shields, Jeffrey D

    2012-02-01

    Hematodinium sp. infections are relatively common in some American blue crab (Callinectes sapidus) populations in estuaries of the western Atlantic Ocean. Outbreaks of disease caused by Hematodinium sp. can be extensive and can cause substantial mortalities in blue crab populations in high salinities. We examined several species of crustaceans to determine if the same species of Hematodinium that infects C. sapidus is found in other crustaceans from the same localities. Over a 2-yr period, 1,829 crustaceans were collected from the Delmarva Peninsula, Virginia, examined for the presence of infections. A portion of the first internal transcribed spacer (ITS1) region of the ribosomal RNA (rRNA) gene complex from Hematodinium sp. was amplified and sequences were compared among 35 individual crustaceans putatively infected with the parasite, as determined by microscopic examination, and 4 crustaceans putatively infected based only on PCR analysis. Of the 18 crustacean species examined, 5 were infected with Hematodinium sp. after microscopic examination and PCR analysis, including 3 new host records, and an additional species was positive only via PCR analysis. The ITS1 rRNA sequences of Hematodinium sp. from the infected crustaceans were highly similar to each other and to that reported from C. sapidus (>98%). The similarity among these ITS1 sequences and similarities in the histopathology of infected hosts is evidence that the same species of Hematodinium found in C. sapidus infects a broad range of crustaceans along the Delmarva Peninsula. Our data indicate that the species of Hematodinium found in blue crabs from estuaries along the east coast of North America is a host generalist, capable of infecting hosts in different families within the Order Decapoda. Additionally, evidence indicates that it may be capable of infecting crustaceans within the Order Amphipoda.

  13. Biodiversidad, morfometría y alimentación de los cangrejos del género Callinectes (Decapoda: Portunidae en Santiago de Cuba

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    Liliana Gómez Luna

    2009-09-01

    Full Text Available El objetivo del presente trabajo es el estudio del género Callinectes en la zona costera del municipio de Santiago de Cuba. Se realizó una recolecta de 257 ejemplares durante marzo de 2007 a abril de 2008, en ocho estaciones a lo largo de la costa. El género resultó estar ampliamente distribuido en el litoral y representado al menos por cuatro especies: C. sapidus, C. similis, C. rathbunae y C. larvatus, las dos últimas no incluidas en la lista publicada de crustáceos (Crustacea: Decapoda cubanos. Las especies más abundantes resultaron ser C. sapidus y C. similis. C. rathbunae fue la más ampliamente distribuida. El análisis del contenido estomacal permitió identificar nueve categorías alimentarias, siendo las más importantes los peces y las macroalgas. Las especies de Callinectes tienen un amplio espectro trófico; comen no sólo lo disponible, sino también lo más abundante.Biodiversity, morphometry and diet of Callinectes crabs (Decapoda: Portunidae in Santiago de Cuba. On the basis of fishery intensity and proximity to river mouth, a total of 257 swimming Callinectes crabs were collected from March 2007 to April 2008 in eight localities. Captures were made with hanging nets, to a maximal depth of 1.5m, establishing a top time of 2 hr. The genus was widely distributed, and it is represented at least by four species: C. sapidus, C. similis, C. rathbunae and C. larvatus, the last two not included in the last list of Cuban crustaceans (Crustacea: Decapoda. The size and shape of the gonopods were very useful as taxonomical criteria, considering the prevalence of males. The most abundant species were C. sapidus (47.08%, and C. similis (30.35%. C. rathbunae, which was identified at 75% (6 of the localities, showed a better distribution, followed by C. sapidus, which appears in the 63% (5. Sex rate (Rs by species (male:female was 6.20 for C. rathbunae, 3.58 for C. similis, 1.40 for C. larvatus and 0.40 for C. sapidus. The most abundant

  14. Lilliput effect in a retroplumid crab (Crustacea: Decapoda) across the K/Pg boundary

    Science.gov (United States)

    Martínez-Díaz, José Luis; Phillips, George E.; Nyborg, Torrey; Espinosa, Belinda; Távora, Vladimir de Araújo; Centeno-García, Elena; Vega, Francisco J.

    2016-08-01

    The genus Costacopluma (Brachyura: Decapoda: Retroplumidae) had a wide distribution during the early Paleogene and is currently represented by 14 species across the Late Cretaceous and early Paleogene. Described early Paleogene species have a smaller mean body size compared to Campanian-Maastrichtian populations of Africa, northeastern Mexico, and southeastern United States. Originally described from the Paleocene and Eocene of Alabama, Costacopluma grayi Feldmann and Portell, 2007, is now documented from the uppermost Maastrichtian (66.2 Ma) of northeastern Mexico and Mississippi and Lower Paleocene of Arkansas, all representing medium size specimens. The morphological features of latest Maastrichtian (66.2 Ma) individuals are identical to those observed among populations of C. grayi from the Paleogene of Alabama and Arkansas, which have a smaller mean size. This size reduction, or dwarfism, in C. grayi across the K-Pg boundary is an example of the Lilliput effect. Dwarfism has been documented in several invertebrate groups as a response to environmental stress, but this is the first record of the Lilliput effect in brachyuran crustaceans. The stratigraphic and geographic range for Costacopluma mexicana Vega and Perrilliat, 1989, is extended to the upper Campanian in northeastern Mexico and lower Maastrichtian in Mississippi and is suggested as a possible ancestor of C. grayi. Different preservational modes for this species in northeastern Mexico are discussed.

  15. Theoretical basis of allometric relationships in juvenile brachyura ...

    African Journals Online (AJOL)

    ... juvenile Brachyura (crabs) was studied theoretically to assess the relevance of the allometric factor and the validity of the condition factor as these factors are often not determined because most fishery investigations are conducted for adult population. The allometric factor appears to be the main parameter in the equation: ...

  16. Phylogeography, cave invasion and diversification of the Philippine Sundathelphusa (Decapoda: Brachyura: Parathelphusidae)

    Science.gov (United States)

    Husana, D.; Haga, T.; Kase, T.; Yamamuro, M.

    2010-12-01

    Availability of resources influences the opportunistic characters of animals to migrate and colonize a specific habitat while the organism’s geographic isolation causes speciation and diversification. Stable isotope signatures (δ13C and δ15N) elucidate the nutrient source of cavernicolous animals. Mitochondrial DNA sequences of the large subunit rRNA (16S rRNA) revealed multiple colonizations of caves by the genus Sundathelphusa as well as its phylogenetic relationships. Overall molecular phylogeny of the freshwater crabs recovered one major clade of the Philippine Sundathelphusa consisting nine clusters/subclades distributed to various regions in the archipelago. Molecular clock estimation based on the substitution rate of 0.88% per million years established for the grapsid crab genus Sesarma suggested that the diversification of Sundathelphusa species in the Philippines could have started during the late Miocene epoch ca. 5.92 mya. Speciation events coincide with the timing of the eustatic sea level fluctuation and geologic changes in the Philippine archipelago. The sea level fluctuation and subsequent geologic changes must have caused geographic isolation of the species. This appears to be the most plausible explanation for the high diversity of Sundathelphusa species in the Philippines where its congeners inhabit a wide range of habitats, ranging from epigean to hypogean domain.

  17. A new species of land crab, Sesarma Say, 1817 (Decapoda, Brachyura), from the Pleistocene of Jamaica

    NARCIS (Netherlands)

    Collins, J.S.H.; Mitchell, S.F.; Donovan, S.K.

    2009-01-01

    Terrestrial arthropods are poorly known from the Cenozoic of Jamaica. A probably Late Pleistocene land crab, Sesarma primigenium sp. nov., is described from the Western Cement Company Cave, parish of St. Elizabeth, southwestern Jamaica. The same species is also tentatively recognised from the Late

  18. New record of Clypeasterophilus stebbingi (Rathbun, 1918 (Decapoda: Brachyura: Pinnotheridae from the east coast of Nicaragua

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    Michel E. Hendrickx

    Full Text Available The pinnotherid crab Clypeasterophilus stebbingi (Rathbun, 1918, previously known from Florida, USA, Colombia and Brazil, is reported for the first time from Central America, on the east coast of Nicaragua. A single female specimen was collected on the sand dollar Clypeaster subdepressus (Gray, 1825, at 4 m depth, among turtle grass on sandy bottom. The specimen fits well with the description provided by M.J. Rathbun and observations made on photographs of the male holotype. Comparison of the material described from Florida (including the type material, Colombia and Brazil to the Nicaragua specimen, however, indicates that C. stebbingi shows some variation in the shape of the third maxilliped.

  19. Juvenile development of Callinectes danae Smith, 1869 (Crustacea, Decapoda, Brachyura, Portunidae under laboratory conditions

    Directory of Open Access Journals (Sweden)

    EDUARDO A. BOLLA Jr

    2014-03-01

    Full Text Available The juvenile development of Callinectes danae was investigated from megalopae obtained in neuston samples at Ubatuba, São Paulo, Brazil. The individuals were raised in the laboratory under constant temperature (25 ± 1°C, filtered sea water from the collection location (35‰, and natural photoperiod. Newly hatched Artemia sp. nauplii were offered as food on a daily basis and ornamental-fish food was also provided for the juveniles from the 4th stage on. Twelve stages of the juvenile phase were obtained. The main morphological features that allowed recognition of the first juvenile stage were drawn and described. All the subsequent stages obtained were examined and measured, and the main changes in relation to the first stage were recorded. Sexual dimorphism becomes apparent from the fourth juvenile stage onwards. Some appendages and morphological features proved to be of great importance in the identification of species, including the number of segments of the antennal flagellum and the number of setae on the maxilla and on the 1st, 2nd and 3rd maxillipeds. These can probably be used for future comparisons and species identifications.

  20. Juvenile development of Callinectes danae Smith, 1869 (Crustacea, Decapoda, Brachyura, Portunidae) under laboratory conditions.

    Science.gov (United States)

    Bolla, Eduardo A; Fransozo, Vivian; Negreiros-Fransozo, Maria Lucia

    2014-03-01

    The juvenile development of Callinectes danae was investigated from megalopae obtained in neuston samples at Ubatuba, São Paulo, Brazil. The individuals were raised in the laboratory under constant temperature (25 ± 1°C), filtered sea water from the collection location (35‰), and natural photoperiod. Newly hatched Artemia sp. nauplii were offered as food on a daily basis and ornamental-fish food was also provided for the juveniles from the 4th stage on. Twelve stages of the juvenile phase were obtained. The main morphological features that allowed recognition of the first juvenile stage were drawn and described. All the subsequent stages obtained were examined and measured, and the main changes in relation to the first stage were recorded. Sexual dimorphism becomes apparent from the fourth juvenile stage onwards. Some appendages and morphological features proved to be of great importance in the identification of species, including the number of segments of the antennal flagellum and the number of setae on the maxilla and on the 1st, 2nd and 3rd maxillipeds. These can probably be used for future comparisons and species identifications.

  1. Embryonic development of the southern surf crab Ovalipes trimaculatus (Decapoda: Brachyura: Portunoidea

    Directory of Open Access Journals (Sweden)

    Antonela Martelli

    2016-11-01

    Full Text Available The embryogenesis of Ovalipes trimaculatus, a member of the highly valued portunid swimming crabs, was studied under nearly constant temperature (13±1°C, salinity (33 and photoperiod (14 h light:10 h dark conditions. A five-stage scale of embryonic development was defined for the species. Time required to complete development averaged 35.7±2.11 days, showing no significant differences between embryos located in inner, middle and outer portions of the egg mass. The egg chorion was rounded and showed the highest growth in diameter between stages I (morula-blastula-gastrula and II (primordium of larval structure and between stages III (appendage formation and IV (eye formation. Results reported here provide a baseline for optimizing hatchery operations and useful information for management purposes.

  2. Etisus evamuellerae, a new xanthid crab (Decapoda, Brachyura) from the Middle Miocene of Austria and Hungary

    Science.gov (United States)

    Hyžný, M.; van Bakel, B.W.M.; Guinot, D.

    2015-01-01

    On the basis of several carapaces, a new species of xanthid crab, Etisus evamuellerae, is described from the Middle Miocene of the Vienna (Austria) and Great Hungarian basins. It differs from the coeval xanthids, Xantho moldavicus and Pilodius vulgaris, in having a distinctly protruding front and comparatively longer carapace. Contrary to those two species, the new one makes up for just a small percentage in the decapod crustacean assemblages studied. PMID:25983383

  3. Two records of Macrophthalmus Desmarest, 1823 (Decapoda: Brachyura: Thoracotremata from the NW of the Arabian Gulf

    Directory of Open Access Journals (Sweden)

    Amaal Gh. Yasser

    2013-09-01

    Full Text Available Specimens of two crabs Macrophthalmus dentipes Lucas, 1836 and Macrophthalmus laevis A. Milne-Edwards, 1867 were collected from the intertidal zone of the lower reaches of Shatt Al-Arab at Fao region, Basrah, Iraq, 2012. A note on the morphological features of these two species and a photograph is provided to confirm the identification of the crabs.

  4. First record of Grapsus grapsus (Linnaeus, 1758 (Brachyura: Decapoda: Grapsidae in freshwater habitat

    Directory of Open Access Journals (Sweden)

    Tammy Iwasa Arai

    2017-12-01

    Full Text Available Abstract The sally lightfoot crab, Grapsus grapsus (Linnaeus, 1758, has been recorded from the oceanic islands off Brazil, as well as from the Caribbean, in coastal rocky shores in the Eastern Pacific, and in Galapagos. We report the first observation of G. grapsus in freshwater habitat, registered in a stream at Trindade Island, Brazil.

  5. Relative growth of Acantholobulus schmitti (Rathbun, 1930 (Crustacea, Brachyura, Panopeidae at Guaratuba Bay, southern Brazil

    Directory of Open Access Journals (Sweden)

    SF Frigotto

    Full Text Available A study on the relative growth of the crab Acantholobulus schmitti (Rathbun, 1930 (Decapoda, Brachyura, Panopeidae was performed with allometric techniques. The species is associated with lanterns used for oyster farming at Guaratuba Bay, southern Brazil. Crabs were obtained from five lanterns from March through December 2009. The following morphological characteristics were measured: the carapace width (CW, the height and length of the major cheliped propodus (LPH and LPL, the width of the female abdomen at the base of the 4th somite (AW and the length of the male gonopod (GL. A total of 1,004 individuals of A. schmitti were measured, of which 451 were males, 323 non-ovigerous females and 230 ovigerous females. The CW of the males varied from 1.87 to 11.86 mm, that of non-ovigerous females from 1.44 to 8.77 mm and that of ovigerous females from 4.09 to 11.12 mm. The plot of LPH against CW showed a mean inflection point at 3.67 mm CW for the males. This result indicated that male crabs are juvenile below this value and adult above it. For the females, the corresponding inflection point occurred at 3.36 mm CW in the plot of AW against CW. These results, CW x LPH and CW x AW, highlight the importance of chelae enlargement at the onset of maturation in males. Similarly, the widening of the abdomen in females is required to accommodate egg clutches during the reproductive period. Heterochely was recorded in both sexes. Right-handed crabs were more frequent than left-handed ones, with percentages of 75.8% in males and 82.7% in females. The population of A. schmitti from Guaratuba Bay becomes sexually mature at an earlier age than A. schmitti from the coast of São Paulo state. The lanterns used in oyster farming furnish a safe habitat in which these crabs can spend their entire benthic life.

  6. Detection of naphthalene by the blue crab, Callinectes sapidus

    Energy Technology Data Exchange (ETDEWEB)

    Pearson, W.H.; Olla, B.L.

    1979-03-01

    Increases in the antennular flicking rate indicated that blue crabs, Callinectes sapidus, detected the petroleum hydrocarbon naphthalene. A low incidence of aggressive displays but no food searching or gathering followed naphthalene detection. The results suggest that the chemosensory abilities of decapod crustaceans cover a broader range of substances than previously supposed.

  7. Observações sobre a ocorrência de Pilumnus floridanus Stimpson (Decapoda, Brachyura, Pilumnidae na costa do Rio de Janeiro, Brasil Remarks about the Pilumnus floridanus Stimpson (Decapoda, Brachyura, Pilumnidae occurrence in Rio de Janeiro coast, Brazil

    Directory of Open Access Journals (Sweden)

    Lydia M. S. Oliveira

    2006-12-01

    Full Text Available Os espécimes foram coletados na Baía de Sepetiba, Rio de Janeiro no inverno. Sua distribuição era conhecida para Off Cape Lookout, Carolina do Norte até a Flórida, Golfo do México, Canal de Yucatan, Honduras, Antilhas, Venezuela e Brasil (Alagoas, Sergipe e Bahia.The specimens were collected in Sepetiba Bay during the winter. Their distribution was formerly known from Off Cape Lookout, North Carolina until Florida, Gulf of Mexico, Yucatan Channel, Honduras, Antilles, Venezuela and Brazil (Alagoas, Sergipe and Bahia.

  8. Sacculina nectocarcini, a new species of rhizocephalan, a new species of rhizocephalan(Cirripedia: Rhizocephala) parasitising the red rock crabNectocarcinus integrifrons (Decapoda: Brachyura: Portunidae)(Decapoda: Brachyura: Portunidae)

    DEFF Research Database (Denmark)

    Gurney, Robert H.; Rybakov, Alexey V.; Høeg, Jens Thorvald

    2006-01-01

    The parasitic barnacles, Rhizocephala, are a little known group within Australia with only seven described species from a coastline stretching approximately 59763 km. This study describes a new species of Rhizocephala, Sacculina nectocarcini. The description is based on a unique combination...... of features pertaining to the structure of the mantle papillae, the retinaculae and the male receptacles. Biological notes, prevalence and intensity of infection are reported for this rhizocephalan, infesting the red rock crab, Nectocarcinus integrifrons, collected from Western Port, Victoria, Australia....

  9. A new species of Malayopotamon Bott, 1968 (Crustacea: Decapoda: Brachyura: Potamidae), a freshwater crab from northern Sumatra, Indonesia.

    Science.gov (United States)

    Ng, Peter K L

    2014-02-28

    A new species of potamid freshwater crab, Malayopotamon weh sp. nov., is described from the island of Pulau Weh off northern Sumatra, Indonesia. The species superficially resembles three Sumatran species: M. batak Ng & Wowor, 1991, M. tobaense (Bott, 1968), and M. turgeo Ng & Tan, 1999, but can easily be distinguished by various carapace as well as gonopod characters.

  10. Identification of European species of Maja (Decapoda: Brachyura: Majidae: RFLP analyses of COI mtDNA and morphological considerations

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    Guillermo Guerao

    2011-02-01

    Full Text Available Four species of crabs of the genus Maja have been described along the European coast: M. brachydactyla, M. squinado, M. goltziana and M. crispata. The commercially important species M. brachydactyla and M. squinado achieve the largest body sizes and are the most similar in morphology, and are therefore easily confused. The four species of Maja were identified using a novel morphometric index and a polymerase chain reaction followed by restriction fragment length polymorphism analysis (RFLP. The relationship between carapace length and the distance between the tips of antorbital spines was used to distinguish adults of M. brachydactyla and M. squinado. PCR-RFLP analysis of a partial sequence of the mitochondrial cytochrome oxidase type I (COI revealed that the four species of the genus Maja can be unambiguously discriminated using the combination of restriction endonucleases enzymes HpyCH4V and Ase I. The molecular identification may be particularly useful in larvae, juvenile and young crabs, when the morphological differences found in adults are not applicable.

  11. Relative growth, sexual dimorphism and morphometric maturity of Trichodactylus fluviatilis (Decapoda: Brachyura: Trichodactylidae from Santa Terezinha, Bahia, Brazil

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    Tiago Rozário da Silva

    2014-02-01

    Full Text Available Freshwater crabs are important elements in the aquatic biota of brooks, rivers, lakes and ponds, from both ecological and the socio-economic aspects. Trichodactylidae comprises 51 endemic species from the Neotropical region. Among all the species of this family, Trichodactylus fluviatilis Latreille, 1828 has the widest geographic distribution throughout Brazil. Despite that, there are few published contributions on the biology of this species. The present study investigated the following aspects of T. fluviatilis: relative growth, mean size at onset of morphometric maturity, sexual dimorphism, laterality and heterochely. Specimens were collected monthly from September 2010 through August 2011, from the Velha Eugênia Brook, municipality of Santa Teresinha, State of Bahia. Carapace width (CW, carapace length (CL, major cheliped length (MaCL and minor cheliped length (MiCL, major cheliped height (MaCH and minor cheliped height (MiCH, and width of the fifth abdominal segment (5AB were measured to evaluate the presence of sexual dimorphism; the major difference between the sexes was in the CW vs. 5AB ratio. Heterochely was observed in males and females,with the right cheliped larger than the left in 89% of males and 81% of females. Crab size at the onset of morphometric maturity (= puberty molt was estimated based on the ratio between CL, cheliped dimensions, 5AB and CW (independent variable. Females were larger than males when they reached morphometric maturity in all studied relations. We recommend the use of chelipeds and abdominal width relationships to estimate the size at the morphometric maturity in males and females, respectively.

  12. Two new species of freshwater crabs of the genus Sundathelphusa Bott, 1969 (Decapoda: Brachyura: Gecarcinucidae) from caves in Luzon, Philippines.

    Science.gov (United States)

    Husana, Daniel Edison M; Yamamuro, Masumi; Ng, Peter K L

    2014-06-17

    Two cave species of Sundathelphusa are described from a karst area in southern Luzon, Philippines. Both species have elongated ambulatory legs but the eyes and carapace pigmentation are well developed, indicating they are not troglobites. Sundathelphusa danae sp. nov. is superficially more similar to S. longipes (Balss, 1937) than to S. holthuisi Ng, 2010, which was described from the same locality. Sundathelphusa danae sp. nov. is distinguished from its closest congeners by its strongly convex anterolateral margin, more swollen branchial regions, possession of a complete frontal median triangle, laterally inflated subbranchial region and the more slender ambulatory legs. Sundathelphusa vienae sp. nov. is unusual among Sundathelphusa species in that its carapace is more quadrate, with the slender and almost straight male first gonopod tapered and having a pointed terminal segment. 

  13. Great unexpected differences between two populations of the intertidal crab Neohelice granulata inhabiting close but contrasting habitats (Crustacea: Decapoda: Brachyura)

    National Research Council Canada - National Science Library

    Eduardo D. Spivak; Claudia C. Bas; Tomás A. Luppi

    2016-01-01

    ...), are separated by only 190 km. They inhabit bays that drain into a Patagonian gulf in the southernmost limit of the geographical distribution of the species: San Antonio Bay (SAO) and San José Gulf (RSJ...

  14. A new species of portunid crab of the genus Charybdis (De Haan, 1833) (Crustacea: Decapoda: Brachyura) from Goa, India

    Digital Repository Service at National Institute of Oceanography (India)

    Padate, V.P.; Rivonker, C.U.; Anil, A.C.; Sawant, S.S.; Venkat, K.

    . Material and methods Abbreviations. The following abbreviations are used: CL- carapace length; CW- extreme width of carapace; LC- length of left cheliped; RC-length of right cheliped; OD- orbital diameter; IS- inter-orbital space/ Frontal width; G1... Charybdis, De Haan 1833, pp. 9-10. (type species: Cancer sexdentatus Herbst, 1783 (a junior subjective synonym of Cancer feriatus Linnaeus, 1758)) The genus Charybdis is characterized by the fronto-orbital border being decidedly less than the greatest...

  15. Experimental predatory behavior of the stone crab Eriphia verrucosa (Forskål, 1775 (Decapoda, Brachyura, Eriphiidae

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    Marta Pérez-Miguel

    2017-12-01

    Full Text Available Abstract Predatory behavior of the crab Eriphia verrucosa (Forskål, 1775 on the oyster Magallana angulata (Lamarck, 1819, the mussel Mytilus galloprovincialis Lamarck, 1819, the limpet Patella depressa Pennant, 1777, the hermit crab Clibanarius erythropus (Latreille, 1818, the gastropod Phorcus lineatus (da Costa, 1778 and the sea urchin Paracentrotus lividus (Lamarck, 1816 was experimentally studied. Taken into account that the sampled population of E. verrucosa was infected with the parasitic barnacle Sacculina eriphiae Smith, 1906, four predator categories were established regarding crab sex and parasite presence: uninfected and infected males and females, respectively. These four crab categories were fed on the six preys offered, but prey remains suggest that the crab obtains the flesh of each prey using different methods of attack. Irrespective of the prey species, uninfected females consistently presented lower percentages of crab feeding and total consumption. On average, it was found that a significantly higher percentage of crabs feed on M. galloprovincialis, P. depressa and Ph. lineatus than on the other three prey species (chi-squared test, p < 0.05. In terms of mean eaten biomass, the preys most eaten were P. lividus and P. depressa and the least eaten was C. erythropus (ANOVA test, p < 0.05. For crabs feeding on M. galloprovincialis and Ph. lineatus there was a significant positive correlation (p < 0.05 between the predator size and prey size, as well as between the predator size and total prey consumption. According to these results, potential effects of this top predator on intertidal communities are also discussed.

  16. Local knowledge of traditional fishermen on economically important crabs (Decapoda: Brachyura in the city of Conde, Bahia State, Northeastern Brazil

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    de Magalhães Henrique

    2012-07-01

    Full Text Available Abstract Background This article records the traditional knowledge of crab gatherers in the city of Conde, in the North Coast Region of Bahia State, Northeastern Brazil. Methods Data on biological and ecological aspects of economically important brachyuran crustaceans have been obtained from semi-structured interviews and in loco observations conducted from September 2007 to December 2009. A total of 57 fishermen of both genders, aged between 10 and 78 years have been interviewed (individually or collectively in different contexts; interviewees were asked about aspects such as external morphology, life cycle, trophic ecology, and spatial and temporal distribution of the major economically important brachyuran crustaceans in the region. Seven fishing communities were visited: Siribinha, Sítio do Conde, Poças, Ilha das Ostras, Cobó, Buri and Sempre Viva. Data were analyzed by comparing the information provided by participants with those from the specialized academic literature. Results The results show that artisanal fishermen have a wide ranging and well-grounded knowledge on the ecological and biological aspects of crustaceans. Crab gatherers of Conde know about growth and reproductive behavior of the animals they interact with, especially with regard to the three major biological aspects: “molt”, “walking dance” and “spawning”. Conclusion This knowledge constitutes an important source of information that should be considered in studies of management and sustainable use of fishery resources in the North Coast Region of Bahia State.

  17. A new species of the genus Kingsleya from Amazonia, with a modified key for the Brazilian Pseudothelphusidae (Crustacea: Decapoda: Brachyura)

    NARCIS (Netherlands)

    Magalhaes, C.

    1990-01-01

    Abstract: A new species of Pseudothelphusid crab, Kingsleya besti spec. nov., is described from Serra do Curicuriari, northwest of the State of Amazonas, Brazil. A modified dey for identification of the Pseudothelphusid crabs occurring in Brazil is given. Resumo: É descrita uma nova espécie de

  18. Insular species of Afrotropical freshwater crabs (Crustacea: Decapoda: Brachyura: Potamonautidae and Potamidae) with special reference to Madagascar and the Seychelles

    NARCIS (Netherlands)

    Cumberlidge, N.

    2008-01-01

    The evolutionary relationships between island and mainland faunas of the 24 species of insular freshwater crabs in the Afrotropical region are reviewed in the light of phylogenetic studies. Twenty insular species of freshwater crabs are endemic, and four are also found on the neighboring mainland of

  19. Comparative study of the morphology of the female seminal receptacles of Ilia nucleus and Persephona mediterranea (Decapoda, Brachyura, Leucosiidae).

    Science.gov (United States)

    Hayer, Sarah; Köhnk, Stephanie; Schubart, Christoph D; Boretius, Susann; Gorb, Stanislav N; Brandis, Dirk

    2017-03-01

    Because of the poor knowledge of the morphology of the female reproductive organs of most brachyuran crabs, this study investigated two Atlantic representatives of the family Leucosiidae, Ilia nucleus (Linnaeus, 1758) and Persephona mediterranea (Herbst, 1794), using histological methods and magnetic resonance imaging (MRI). While the vagina conforms to the concave type, the arrangement of the two chambers of the seminal receptacle differs strongly from that of other eubrachyuran sperm storage organs. Both chambers are oriented laterally within the crab's body. This is in contrast to the dorso-ventral orientation described in most other known brachyuran crabs. The lateral chamber is covered by cuticle, whereas the medial chamber is covered by a holocrine glandular epithelium. The oviduct connection is located ventrally, posterior to the vagina. The oviduct orifice is characterized by a transition from the epithelium lining the oviduct to the seminal receptacle's holocrine glandular epithelium. Moreover, muscle fibres are attached to the oviduct orifice and to the sternal cuticle. This musculature can be interpreted as an important feature in the fertilization and egg-laying process by supporting and controlling the inflow of eggs into the seminal receptacle lumen. The results of this study are compared to the morphology of the seminal receptacle of another leucosiid crab, Ebalia tumefacta (Montagu, 1808), and to those of other known eubrachyuran crabs. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Morfología comparada del esqueleto axial en la familia de cangrejos de agua dulce Trichodactylidae (Crustacea: Decapoda: Brachyura

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    Manuel Pedraza

    2015-07-01

    Full Text Available El esqueleto axial es una estructura compleja de origen cuticular, formada a partir de las láminas esclerotizadas de los esternitos y pleuritos torácicos así como por sus invaginaciones internas. Los caracteres de la parte ventral del esqueleto axial, que se reflejan externamente en las líneas de sutura del esternón torácico, son criterios importantes en el actual sistema de supragenerico de Trichodactylidae: (Trichodactylinae y Dilocarcininae (Dilocarcinini + Valdiviini. Con el objetivo de probar esa hipótesis de clasificación fue realizado el estudio anatómico del esqueleto axial de 43 individuos adultos, tanto machos como hembras, pertenecientes a 17 especies de 10 géneros de Trichodactylidae. Para la preparación del material, los individuos fueron diseccionados y sus esqueletos sumergidos, por un periodo de 24 a 48 horas, en una solución caliente de Hidróxido de Potasio (KOH. El análisis anatómico permitió describir un grupo de variaciones en la forma, orientación y conformación del esternón torácico, techo pleural, fragmas ventrales y dorsales, así como de la lámina de unión. De acuerdo con esas características las especies estudiadas fueron reunidas en tres grupos, siendo esas agrupaciones consistentes con la actual propuesta de clasificación de Trichodactylidae en subfamilias y tribus. El presente trabajo resalta la importancia informativa de la morfología del esqueleto axial para la taxonomía de Trichodactylidae.

  1. Reproductive cycle of the swimming crab Portunus spinimanus Latreille (Crustacea, Decapoda, Brachyura from Ubatuba, São Paulo, Brazil

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    Sandro Santos

    1999-01-01

    Full Text Available This study analyses some reproductive aspects of Portunus spinimanus Latreille, 1819, a crab usually commercialized in Ubatuba region. Monthly otter-trawl collections were taken for two years along the northern coast of São Paulo State, Brazil. The reproductive period was studied based on the frequency of ovigerous females in the population along the year; the frequency of physiologically mature swimming crabs and the type of spawn. The condition of development of ovigerous females ovary was associated with the stage of eggs development. This species presents continuous reproduction in Ubatuba region and total spawn, but with successive broods, which suggests a multiple spawn during the reproduction period.

  2. Description of a new genus of Cryptochiridae (Decapoda: Brachyura associated with Siderastrea (Anthozoa: Scleractinia, with notes on feeding habits

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    Marcelle F.S. Badaro

    2012-08-01

    Full Text Available Members of the Cryptochiridae are small gall-crabs that live as obligate symbionts of scleractinian corals. Only two species have so far been recorded in the western Atlantic Ocean. Herein a new Cryptochiridae genus and species is described, and new information is added on the life history of cryptochirids. The new genus is characterized by having the carapace with the lowest deflection angle among the genera, and also shows the following features: thoracic sternite 4 with setules and constriction smaller than half of the width of the basis, anterior margin curved with apical row of granules; third maxilliped with subcircular exopod reaching medially the lateral margin of the ischium; pereiopod 2 with prominent distomesial and anterolateral expansion on the merus, propodus almost twice larger than dactylus; thoracic sternite 7 with complete medial suture, female pleopod 3 uniramous with longitudinal opening. Male first pleopod straight with subdistal curvature of approximately 90°. Individuals belonging to the new genus are found in galls in massive corals although this structure is cited as being characteristic of ramified corals. The long plumose setae of the maxilliped 3 suggest a filter-feeding function, but the toothless chelae suggest that they are used to gather mucus.

  3. Histopathological characterization and in situ detection of Callinectes sapidus reovirus.

    Science.gov (United States)

    Tang, Kathy F J; Messick, Gretchen A; Pantoja, Carlos R; Redman, Rita M; Lightner, Donald V

    2011-11-01

    A reovirus (tentatively designated as Callinectes sapidus reovirus, CsRV) was found in the blue crabs C. sapidus collected in Chesapeake Bay in 2005. Histological examination of hepatopancreas and gill from infected crabs revealed eosinophilic to basophilic, cytoplasmic, inclusions in hemocytes and in cells of connective tissue. A cDNA library was constructed from total RNA extracted from hemolymph of infected crabs. One clone (designated as CsRV-28) with a 532-bp insert was 75% identical in nucleotide sequence (and 95% similar in translated amino acid sequence) to the quanylytransferase gene of the Scylla serrata reovirus (SsRV). The insert of CsRV-28 was labeled with digoxigenin-11-dUTP and hybridized to sections of hepatopancreas and gill of infected C. sapidus, this probe reacted to hemocytes and cells in the connective tissue. No reaction was seen in any of the tissues prepared from uninfected crabs. Thus, this in situ hybridization procedure can be used to diagnose CsRV. Copyright © 2011 Elsevier Inc. All rights reserved.

  4. Comparative brain architecture of the European shore crab Carcinus maenas (Brachyura) and the common hermit crab Pagurus bernhardus (Anomura) with notes on other marine hermit crabs.

    Science.gov (United States)

    Krieger, Jakob; Sombke, Andy; Seefluth, Florian; Kenning, Matthes; Hansson, Bill S; Harzsch, Steffen

    2012-04-01

    The European shore crab Carcinus maenas and the common hermit crab Pagurus bernhardus are members of the sister taxa Brachyura and Anomura (together forming the taxon Meiura) respectively. Both species share similar coastal marine habitats and thus are confronted with similar environmental conditions. This study sets out to explore variations of general brain architecture of species that live in seemingly similar habitats but belong to different major malacostracan taxa and to understand possible differences of sensory systems and related brain compartments. We examined the brains of Carcinus maenas, Pagurus bernhardus, and three other hermit crab species with immunohistochemistry against tyrosinated tubulin, f-actin, synaptic proteins, RF-amides and allatostatin. Our comparison showed that their optic neuropils within the eyestalks display strong resemblance in gross morphology as well as in detailed organization, suggesting a rather similar potential of processing visual input. Besides the well-developed visual system, the olfactory neuropils are distinct components in the brain of both C. maenas and P. bernhardus as well as the other hermit crabs, suggesting that close integration of olfactory and visual information may be useful in turbid marine environments with low visibility, as is typical for many habitats such as, e.g., the Baltic and the North Sea. Comparing the shape of the olfactory glomeruli in the anomurans showed some variations, ranging from a wedge shape to an elongate morphology. Furthermore, the tritocerebrum and the organization of the second antennae associated with the tritocerebrum seem to differ markedly in C. maenas and P. bernhardus, indicating better mechanosensory abilities in the latter close to those of other Decapoda with long second antennae, such as Astacida, Homarida, or Achelata. This aspect may also represent an adaptation to the "hermit lifestyle" in which competition for shells is a major aspect of their life history. The shore

  5. Evaluation of parenteral drugs for anesthesia in the blue crab (Callinectes sapidus).

    Science.gov (United States)

    Quesada, Rolando J; Smith, Christopher D; Heard, Darryl J

    2011-06-01

    The objective of this study was to evaluate the efficacy and safety of several parenteral anesthetics in blue crabs (Callinectes sapidus). Thirty-one animals were administered one or more of the following drugs by injection into the hemolymph (i.v.) through an arthrodial membrane: etomidate, ketamine, lidocaine, pentobarbital, propofol, tiletamine-zolazepam, xylazine, and ketamine-xylazine. A subset of crabs received intracardiac ketamine. Etomidate had no effect. Lidocaine effects were ultrashort (sapidus as well as in other crab species.

  6. Blue crabs Callinectes sapidus as potential biological reservoirs for white spot syndrome virus (WSSV).

    Science.gov (United States)

    Powell, James W B; Browdy, Craig L; Burge, Erin J

    2015-03-09

    White spot syndrome virus (WSSV) is a virulent pathogen of cultured shrimp and was first detected in farms in South Carolina (USA) in 1997 and subsequently in wild shrimp in 1999. We screened groups of 1808 wild Atlantic white shrimp Litopenaeus setiferus and 300 blue crabs Callinectes sapidus collected from South Carolina, Georgia, and Florida for the presence of WSSV using the Shrimple® immunoassay-strip test, with all positives and random subsets of negatives tested by TaqMan real-time PCR and in infectivity bioassays. Of 87 shrimp and 11 crabs that tested positive using the Shrimple® test, only a single C. sapidus was confirmed to be infected with WSSV by PCR and the infectivity bioassay. The data indicate that the prevalence of WSSV in these species is low in these southeastern US regions, but that C. sapidus may serve as a biological reservoir.

  7. First Record of Blue Crab Callinectes sapidus (Rathbun 1896 from the Middle Black Sea Coast

    Directory of Open Access Journals (Sweden)

    Mehmet AYDIN

    2017-12-01

    Full Text Available Two individuals of blue crab Callinectes sapidus (one male and one female were captured by trammel nets on 27 November 2017 and 1 December 2017 between 2 m to 10 m depths from the middle Black Sea coast. The carapace width and weight were measured as 19.4 cm and 449.19 g for the male, and 19.6 cm and 269.39 g for the female specimens. This is the first record for the C. sapidus from the middle Black Sea region.

  8. Crescimento relativo de Macrobrachium acanthurus (Wiegmann, 1836 (Crustacea, Decapoda, Palaemonidae

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    Wagner Cotroni Valenti

    1989-04-01

    Full Text Available In this paper, the relative growth of Macrobrachium acanthurus (Wiegmann, 1836 (Crustacea, Decapoda, Palaemonidae, is presented; the cephalothorax length/abdominal length, telson length/abdominal length and abdominal length/total length relashionships were determined. The adjusted equations showed a isometric growth pattern. There are no changes in these relationships during the animals growth that may be significant in the maximization of the meat's profit.

  9. New localities for Crustacea Decapoda in the Magellan region, southern South America

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    Julio H. Vinuesa

    1999-12-01

    Full Text Available We report the occurrence of Lithodes turkayi (Anomura, Libidoclaea granaria, and C. edwardsii (Brachyura, in the Beagle Channel. This observation extends their range of distribution south of the previously reported limit of the Straits of Magellan. Acanthocyclus albatrossis (Brachyura occurs south of Isla Navarino and also in the Beagle Channel, however in a particular habitat of its northern coast. The occurrence of Lithodes confundens (formerly identified as L. antarcticus north of the eastern entrance of the Straits of Magellan is also reported. We extend its range of distribution northerly and its bathymetric distribution to the intertidal.

  10. Synopsis of biological data on the blue crab, Callinectes sapidus Rathbun

    OpenAIRE

    Millikin, Mark R.; Williams, Austin B.

    1984-01-01

    This synopsis reviews taxonomy, morphology, distribution, life history, commercial hard and soft shell crab fisheries, physiology, diseases, ecology, laboratory culture methodology, and influences of environmental pollutants on the blue crab, Callinecles sapidus. Over 300 selected, published reports up to and including 1982 are covered. (PDF file contains 45 pages.)

  11. TROPHIC ACCUMULATION AND DEPURATION OF MERCURY BY BLUE CRABS (CALLINECTES SAPIDUS) AND PINK SHRIMP (PENAEUS DUORARUM).

    Science.gov (United States)

    Mercury concentrations in blue crabs (Callinectes sapidus) collected from an area of mercury-contaminated sediments in Lavaca Bay, TX, USA, are more than an order of magnitude greater than concentrations in penaeid shrimp from the same area. Laboratory feeding experiments using ...

  12. Influence of salinity on toxicity of cadmium and chromium to the blue crab, Callinectes sapidus

    Energy Technology Data Exchange (ETDEWEB)

    Frank, P.M.; Robertson, P.B.

    1979-01-01

    This study reports the lethal concentrations of cadmium and chromium to juvenile blue crabs, Callinectes sapidus, in acute toxicity tests at three different salinities. This economically important crustacean ranges throughout estuaries from fresh water to marine salinities, and may be subject to heavy metal pollution in industrial areas.

  13. Comparative analyses of olfactory systems in terrestrial crabs (Brachyura: evidence for aerial olfaction?

    Directory of Open Access Journals (Sweden)

    Jakob Krieger

    2015-12-01

    Full Text Available Adaptations to a terrestrial lifestyle occurred convergently multiple times during the evolution of the arthropods. This holds also true for the “true crabs” (Brachyura, a taxon that includes several lineages that invaded land independently. During an evolutionary transition from sea to land, animals have to develop a variety of physiological and anatomical adaptations to a terrestrial life style related to respiration, reproduction, development, circulation, ion and water balance. In addition, sensory systems that function in air instead of in water are essential for an animal’s life on land. Besides vision and mechanosensory systems, on land, the chemical senses have to be modified substantially in comparison to their function in water. Among arthropods, insects are the most successful ones to evolve aerial olfaction. Various aspects of terrestrial adaptation have also been analyzed in those crustacean lineages that evolved terrestrial representatives including the taxa Anomala, Brachyura, Amphipoda, and Isopoda. We are interested in how the chemical senses of terrestrial crustaceans are modified to function in air. Therefore, in this study, we analyzed the brains and more specifically the structure of the olfactory system of representatives of brachyuran crabs that display different degrees of terrestriality, from exclusively marine to mainly terrestrial. The methods we used included immunohistochemistry, detection of autofluorescence- and confocal microscopy, as well as three-dimensional reconstruction and morphometry. Our comparative approach shows that both the peripheral and central olfactory pathways are reduced in terrestrial members in comparison to their marine relatives, suggesting a limited function of their olfactory system on land. We conclude that for arthropod lineages that invaded land, evolving aerial olfaction is no trivial task.

  14. Five new species of freshwater crabs of the genera Ghatiana Pati & Sharma, 2014, and Gubernatoriana Bott, 1970 (Crustacea, Decapoda, Brachyura: Gecarcinucidae Rathbun, 1904) from the Western Ghats, India.

    Science.gov (United States)

    Pati, S K; Thackeray, T; Khaire, A

    2016-02-23

    Five new species of gecarcinucid freshwater crabs, Ghatiana atropurpurea n. sp., Ghatiana splendida n. sp., Gubernatoriana alcocki Pati n. sp., Gubernatoriana thackerayi Pati n. sp., and Gubernatoriana waghi Pati n. sp. are described from the Western Ghats in Maharashtra and Goa states of India. Ghatiana atropurpurea n. sp. can be differentiated from other species of Ghatiana Pati & Sharma, 2014 by the shape and colour of its carapace, and the sigmoid-shaped male first pleopod (G1). Ghatiana splendida n. sp. is separated from its congeners mainly by its long anterolateral margin of the carapace and short terminal article of the G1. Besides, the colour pattern (deep pink carapace and chelipeds, orange ambulatory legs) of G. splendida n. sp. is exceptional among its congeners. Gubernatoriana alcocki n. sp. is unique among species of Gubernatoriana Bott, 1970 due to its inwardly curved tip of the terminal article of the G1. The stout and cone-shaped terminal article of the G1 of G. thackerayi n. sp. is characteristic among its congeners. In addition, G. thackerayi n. sp. has a unique colouration amongst congeners (violet-red carapace and ambulatory legs, orange-red chelipeds). Gubernatoriana waghi n. sp. can be distinguished from its congeners by the quadrate-shaped carapace, narrow frontal margin, pointed tips of the cheliped fingers, short terminal article of the G1 and very short or vestigial terminal article of the male second pleopod (G2). The body colour (burnt orange carapace and ivory coloured chelipeds and ambulatory legs) of G. waghi n. sp. is also important to distinguish live crabs of the genus. Key to the species of Ghatiana and Gubernatoriana is provided.

  15. Significant fluctuations in ecdysteroid receptor gene (EcR) expression in relation to seasons of molt and reproduction in the grapsid crab, Metopograpsus messor (Brachyura: Decapoda).

    Science.gov (United States)

    Shyamal, Sharmishtha; Anilkumar, G; Bhaskaran, R; Doss, G P; Durica, D S

    2015-01-15

    Metopograpsus messor, a brachyuran crab inhabiting the estuaries of North Kerala (India), is a prolific breeder releasing approximately 14-16 broods a year. The present paper reports the sequence information on the DNA binding domain (C domain, DBD), linker (D domain) and ligand binding domain (E domain, LBD) of M. messor ecdysteroid receptor (MmEcR) gene, the first grapsid brachyuran crab EcR examined. We have also measured MmEcR transcript levels in the ovary and the hepatopancreas throughout the annual cycle, with special reference to seasons of molt and reproduction. MmEcR expression in both the tissues is found to be at its peak (P<0.05) in late premolt crabs (January/May, molt/reproduction season); the expression levels are lowest (P<0.05) during June/July, when the females would neither molt nor reproduce (season for molt/reproduction repose). Intermediate levels of expression were found during the breeding season (August/December). Interestingly, this pattern of gene expression is in concordance with the fluctuating ecdysteroid levels of the hemolymph and Y organ secretory activity. The significant levels of fluctuation in the ovarian expression of MmEcR strongly suggest the ovary as a potential target for ecdysteroid action. A season-wise comparison of the gene expression reveals that ovarian MmEcR transcript levels are higher in breeding crabs (August/December) than the non-breeding animals (June/July), implicating a possible ecdysteroid role in reproduction in M. messor. Copyright © 2014 Elsevier Inc. All rights reserved.

  16. Molt and reproduction enhancement together with hemolymph ecdysteroid elevation under eyestalk ablation in the female fiddler crab, Uca triangularis (Brachyura: Decapoda)

    Science.gov (United States)

    Supriya, Nagathinkal T.; Sudha, Kappalli; Krishnakumar, Velayudhannair; Anilkumar, Gopinathan

    2017-05-01

    We present the results of eyestalk extirpation experiments performed on the fiddler crab, Uca triangularis at seasons of molting and reproduction, with a view to have a better understanding of the regulatory mechanisms of these two highly energy-demanding processes. Bilateral eyestalk ablation resulted in precocious acceleration of both molting and reproduction, irrespective of the season at which each experiment was conducted. The rate of accelerated ovarian growth, however, was maximum if the eyestalk ablation was conducted during August-January, the breeding season in the wild, or in February-May (molting-reproductive season), wherein a section of the wild population would be engaged in molting and another section in breeding. The highest degree of precocious molt acceleration, on the other hand, was obtained during June-July when the population was primarily engaged in molting, but with no reproductive activity. The precocious oocyte maturation (due to de-eyestalking) was minimal in June-July. Significantly, the eyestalk ablation also resulted in a dramatic increase in the hemolymph ecdysteroid titer, revealing that a high ecdysteroid titer would have no restraining influence on vitellogenesis. No spawning was, however, observed among de-eyestalked females, even though their final oocyte size surpassed the size of the normal mature oocytes, implying that spawning is not exclusively under the control of eyestalk hormones. A comparative study performed on the vitelline components of the experimentals and the controls revealed that the precociously incorporated yolk under eyestalk ablation was biochemically impoverished. These results indicate that throughout the annual cycle, both the somatic and the reproductive growth of U. triangularis are under the influence of inhibitory principles from the eyestalks. It is also revealing that mere deprival of the inhibitory principles does not culminate into successful vitellogenesis. Arguably, the inhibitory influence from the eyestalks could be a prerequisite for normal healthy maturation of the oocytes and spawning.

  17. The response of digestive enzyme activity in the mature Chinese mitten crab, Eriocheir sinensis (Decapoda: Brachyura, to gradual increase of salinity

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    Ruifang Wang

    2013-06-01

    Full Text Available Mature Chinese mitten crabs, Eriocheir sinensis, were exposed to brackish water or seawater as an obligatory part of their reproductive migration. Physiological and biochemical reorganization were needed to adapt them to this migration. To understand the digestive adjustments of Eriocheir sinensis at biochemical level during this transformation from freshwater to seawater, the response of the activity of five digestive enzymes (amylase, cellulase, pepsin, trypsin and lipase in the hepatopancreas to salinities increasing gradually from 0 (freshwater to 35 (seawater was analysed in mature females and males. Digestive enzymes exhibited significantly higher activities in the hepatopancreas of males than those of females, except lipase. In females, amylase, pepsin and trypsin activities began to decrease significantly as the salinity reached 28, and cellulase activity decreased at 35; in males, a considerable decrease in the activity of digestive enzymes, except lipase, was observed at 21 and higher salinities, while an increase was observed at 14. Reduced enzyme activities at elevated salinities suggest that the digestive capacity of crabs for diets becomes weak, and all these digestive enzymes participated in digestive adjustments during osmoregulation. The initial salinity which induced the decrease of enzyme activity was lower in males than in females, indicating that females were more tolerant to elevated salinities than males from the point of digestive biochemical modulation.

  18. Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab Cyrtograpsus angulatus (Decapoda: Brachyura: Varunidae

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    María Soledad Michiels

    2013-03-01

    Full Text Available We studied the occurrence and characteristics of lipase activity and the response of lipase and proteolytic activity to salinity and dopamine injections in hepatopancreas of the euryhaline crab (Cyrtograpsus angulatus. Lipase activity was maximal at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km=0.019 mM, was higher at 37°C and appeared to be cold tolerant, being also high at 4°C. In 10 psu (hyper-regulation conditions, lipase and proteolytic activity were about 3 and 5 times higher, respectively, than in 35 psu (osmoconformation. In 40 psu (hypo-regulation, lipase activity was about three times higher than in 35 psu, while proteolytic activity was similar. Lipase activity was inhibited in vivo by 10–4 M dopamine in 35 psu but not in 10 or 40 psu. Proteolytic activity was not affected by 10–4 M dopamine. The differential responses of lipase and proteolytic activity to salinity and dopamine suggest the occurrence of distinct digestive adjustments and mechanisms of regulation upon osmoregulatory conditions. This study contributes to a better understanding of the complexity of the biochemical adaptations to salinity in euryhaline crabs. The fact that higher digestive enzyme activities could be associated with a differential digestive capacity potentially leading to enhanced availability of energy substrates is discussed.

  19. Feeding habits of the spider crab Libinia spinosa H. Milne Edwards, 1834 (Decapoda, Brachyura in Ubatuba bay, São Paulo, Brazil

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    Samara de Paiva Barros

    2008-04-01

    Full Text Available The main goal of this study was the identification of the items of the diet of the L. spinosa, based on the stomach contents analysis. The crabs were obtained from Ubatuba region north-eastern shore of São Paulo State. In the laboratory, all the individuals were dissected, the stomach was retreated and fixed in 10% formaline. The alimentary items were identified under stereomicroscope and analysed by the method of Frequency of Occurrence. A total of 194 stomachs was analysed and nine alimentary items were obtained. Unindentified material was found in 98% of analysed stomach and poriferan were present in less then 1% of stomachs. These results pointed a diversified diet explored by this crab, as well as the employment of some different methods for food intake. This suggested that these crabs could occupy different position in the trophic chain.O objetivo deste trabalho é identificar os itens alimentares que compõem a dieta de L. spinosa, por meio da análise do conteúdo estomacal. Os caranguejos foram coletados no litoral norte paulista (23º25’S-45º00’W. No laboratório, todos indivíduos foram dissecados, os estômagos foram retirados e fixados em formol 10%. Os itens alimentares foram identificados sob estereomicroscópio, sendo que para a análise foi utilizado o método Freqüência de Ocorrência. Foram analisados 194 estômagos, nos quais foram encontrados nove itens alimentares. A maior freqüência foi para material não identificado, ocorrendo em 98% dos estômagos analisados e Porifera foi o item com a menor freqüência, ocorrendo em menos de 1% dos estômagos. Com base nos resultados obtidos, sugere-se que o caranguejo L. spinosa apresenta uma dieta diversificada, sugerindo a utilização de diferentes métodos para a obtenção de alimento e por conseqüência podendo ocupar vários níveis na cadeia trófica.

  20. Population biology and distribution of the portunid crab Callinectes ornatus (Decapoda: Brachyura in an estuary-bay complex of southern Brazil

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    Timoteo T. Watanabe

    2014-08-01

    Full Text Available Trawl fisheries are associated with catches of swimming crabs, which are an important economic resource for commercial as well for small-scale fisheries. This study evaluated the population biology and distribution of the swimming crab Callinectes ornatus (Ordway, 1863 in the Estuary-Bay of São Vicente, state of São Paulo, Brazil. Crabs were collected from a shrimp fishing boat equipped with a semi-balloon otter-trawl net, on eight transects (four in the estuary and four in the bay from March 2007 through February 2008. Specimens caught were identified, sexed and measured. Samples of bottom water were collected and the temperature and salinity measured. A total of 618 crabs were captured (332 males, 267 females and 19 ovigerous females, with a sex ratio close to 1:1. A large number of juveniles were captured (77.67%. Crab spatial distributions were positively correlated with salinity (Rs = 0.73, p = 0.0395 and temperature (Rs = 0.71, p = 0.0092. Two peaks of recruitment occurred, in summer and autumn, and ovigerous females were mostly captured during summer, showing a seasonal reproductive pattern. The results showed that C. ornatus uses the bay as a nursery area for juvenile development. Callinectes ornatus is not yet a legally protected species, and the minimum allowed size of crabs caught in the area, although already restricted, should be carefully evaluated since the removal of large numbers of juveniles could negatively impact the local population.

  1. New distribution records of the gall crab Opecarcinus cathyae van der Meij, 2014 (Decapoda: Brachyura: Cryptochiridae) from the Red Sea, Maldives and Japan

    KAUST Repository

    Meij, Sancia E. T.

    2016-11-12

    The gall crab Opecarcinus cathyae van der Meij, 2014 has been reported from various localities in Indonesia and Malaysia. Recent surveys in the Red Sea, Maldives and Japan yielded additional specimens of O. cathyae, considerably expanding the known distribution range of this species to the east and west. The identity of O. cathyae was confirmed based on COI sequence data, revealing identical haplotypes for the Red Sea, Maldivian and Japanese material and three haplotypes in the Indonesian material. Opecarcinus cathyae has one of the widest known recorded distribution ranges for all gall crab species.

  2. Seasonal dynamics of the density of the crab larvae (Decapoda: Brachyura et Anomura) in Minonosok Bay of Pos'eta Bay (Peter the Great Bay, Sea of Japan)

    Science.gov (United States)

    Grigoryeva, N. I.

    2013-03-01

    As the materials for this project, we used data on the distribution of the larvae of the Asian paddle crab Charybdis japonicus (A. Milne-Edwards, 1861); the spider crabs Hyas coarctatus ursinus (= Hyas ursinus) (Rathbun, 1924), Pugettia quadridens (de Haan, 1839), and Pisoides bidentatus (H. Milne-Edwards, 1873); the samurai crab Paradorippe granulata (= Dorippe granulata) (de Haan, 1841); the pea crab Pinnixa rathbuni (Sakai, 1934); and the porcelain crab Pachycheles stevensii (Stimpson, 1858) in Minonosok Bay of Pos'eta Bay obtained during 2000-2002 and in 2004. The planktonic samples were collected from the last third of May to September. The greatest density of the larvae was observed in May, mid-June, late June-early July, and late July-early August. The greatest densities of the crab larvae and the decapod larvae ranged from 20.4 to 48.2 and from 88.4 to 245.3 specimens/m3. The schedule of the crab larvae's occurrence in the plankton is provided for the first time. The distribution of the density showed pronounced patchiness.

  3. Fecundity of Uca uruguayensis and Chasmagnathus granulatus (Decapoda, Brachyura from the "Refugio de Vida Silvestre" Bahía Samborombón, Argentina

    Directory of Open Access Journals (Sweden)

    II. César

    Full Text Available The aim of the present work conducted at the Refugio de Vida Silvestre Bahía Samborombón is to analyse the most relevant aspects of the fecundity of Chasmagnathus granulatus and Uca uruguayensis. Samplings were carried out from March 2001 to February 2003. Ovigerous females of U. uruguayensis (N = 13 and C. granulatus (N = 25 were found during spring and summer, their sizes (CW varied from 9.1 to 11.7 µm for the former species and from 22.8 to 32.4 mm for the latter. The egg diameter in U. uruguayensis ranged from 245 to 260 µm for embryos in the early stage of development and from 250 to 345 µm for those in mid-developmental stage, while in C. granulatus from 250t o 345 µm and from 260 to 365 µm respectively. Fecundity varied from 1126 to 6745 eggs/brood in U. uruguayensis and 15688-57418 eggs/brood in C. granulatus. For those females with broods in mid-developmental stage, several relationships were made. For U. uruguayensis the best correlation coefficients were obtained for the relationships: female weight vs. egg mass weight and carapace width vs. egg mass weight; for C. granulatus the best association was obtained between female size and the egg number and the egg mass weight.

  4. A Kinetic Characterization of the Gill (Na+, K+)-ATPase from the Semi-terrestrial Mangrove Crab Cardisoma guanhumi Latreille, 1825 (Decapoda, Brachyura).

    Science.gov (United States)

    Farias, Daniel L; Lucena, Malson N; Garçon, Daniela P; Mantelatto, Fernando L; McNamara, John C; Leone, Francisco A

    2017-10-01

    We provide a kinetic characterization of (Na+, K+)-ATPase activity in a posterior gill microsomal fraction from the semi-terrestrial mangrove crab Cardisoma guanhumi. Sucrose density gradient centrifugation reveals two distinct membrane fractions showing considerable (Na+, K+)-ATPase activity, but also containing other microsomal ATPases. The (Na+, K+)-ATPase, notably immuno-localized to the apical region of the epithelial pillar cells, and throughout the pillar cell bodies, has an M r of around 110 kDa and hydrolyzes ATP with V M = 146.8 ± 6.3 nmol Pi min-1 mg protein-1 and K M = 0.05 ± 0.003 mmol L-1 obeying Michaelis-Menten kinetics. While stimulation by Na+ (V M = 139.4 ± 6.9 nmol Pi min-1 mg protein-1, K M = 4.50 ± 0.22 mmol L-1) also follows Michaelis-Menten kinetics, modulation of (Na+, K+)-ATPase activity by MgATP (V M = 136.8 ± 6.5 nmol Pi min-1 mg protein-1, K 0.5 = 0.27 ± 0.04 mmol L-1), K+ (V M = 140.2 ± 7.0 nmol Pi min-1 mg protein-1, K 0.5 = 0.17 ± 0.008 mmol L-1), and NH4+ (V M = 149.1 ± 7.4 nmol Pi min-1 mg protein-1, K 0.5 = 0.60 ± 0.03 mmol L-1) shows cooperative kinetics. Ouabain (K I = 52.0 ± 2.6 µmol L-1) and orthovanadate (K I = 1.0 ± 0.05 µmol L-1) inhibit total ATPase activity by around 75%. At low Mg2+ concentrations, ATP is an allosteric modulator of the enzyme. This is the first study to provide a kinetic characterization of the gill (Na+, K+)-ATPase in C. guanhumi, and will be useful in better comprehending the biochemical underpinnings of osmoregulatory ability in a semi-terrestrial mangrove crab.

  5. New records of xanthid crabs Atergatis roseus (Rüppell, 1830 (Crustacea: Decapoda: Brachyura from Iraqi coast, south of Basrah city, Iraq

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    Khaled Khassaf Al-Khafaji

    2017-06-01

    Full Text Available Specimens of the The Brachyuran crab Atergatis roseus (Ruppell, 1830, were collected for first times from Iraqi coast, south Al-Faw, Basrah city, Iraq, in coast of northwest of Arabian Gulf. Morphological features and distribution pattern of this species are highlighted and a figure is provided. The material was mostly collected from the shallow subtidal and intertidal areas using trawl net and hand.

  6. Occurrence of the blue crab Callinectes sapidus, Rathbun, 1896, and its fisheries biology in Bardawil Lagoon, Sinai Peninsula, Egypt

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    Fatma A. Abdel Razek

    2016-06-01

    Full Text Available This study reports the yearly occurrence and the biology of fisheries belonging to the blue crab Callinectes sapidus (Rath. in the catch yield of Bardawil Lagoon (BL in Sinai Peninsula, Egypt over the past 15 years. The study shows that between the years 2000–2015 the crustacean fishery increased (pooling both shrimp and crabs with fluctuations from 40% to 63% of the total production of the lagoon. The crab yield, during this period, increased from 19% to 42% of the total lagoon production. Moreover, the results show that the crab catch of BL is composed mainly of two crab species Portunus pelagicus (L. and C. sapidus (Rath.. The latter is considered the most dominant in the crab yield with 85% of the total crab production, while C. sapidus is only 15%. The size of C. sapidus ranged between 65 and 155 mm (carapace width with a dominance of the class size 105 mm. Furthermore, the ratio of C. sapidus males to females was in favor of males. The regression of width–length relationship showed a marked deviation from the isometric growth. Length–width and body weight regressions also showed deviations from the isometric growth and the analysis of the covariance. This indicates the significant difference between sexes in respect to length weight relationship. The interrelationships between width–length and propodus length and depth of males and with abdomen in females of C. sapidus suggested that in most conditions the relationships were positive and highly significant. This study shows the first insights on the status of C. sapidus in Bardawil Lagoon, Egypt.

  7. Comparison of Three Different Traps for Catching Blue Crab (Callinectes sapidus Rathbun 1896) in Beymelek Lagoon

    OpenAIRE

    ATAR, Hasan Hüseyin

    2014-01-01

    In this study, the capture efficiencies and catch rates of three different traps were compared for the blue crab Callinectes sapidus in Beymelek Lagoon, Antalya, Turkey. All gear was used simultaneously and in the same habitats. The mean catch per unit effort (CPUE) was determined to be significantly higher for hoop nets than for traps. Gear effectiveness was examined by comparing CPUE (CPUE as number of crab per trap per haul, and g per trap haul), mean weight of individual crabs, catch comp...

  8. The Influence of Diet Composition on Fitness of the Blue Crab, Callinectes sapidus.

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    Benjamin A Belgrad

    Full Text Available The physiological condition and fecundity of an organism is frequently controlled by diet. As changes in environmental conditions often cause organisms to alter their foraging behavior, a comprehensive understanding of how diet influences the fitness of an individual is central to predicting the effect of environmental change on population dynamics. We experimentally manipulated the diet of the economically and ecologically important blue crab, Callinectes sapidus, to approximate the effects of a dietary shift from primarily animal to plant tissue, a phenomenon commonly documented in crabs. Crabs whose diet consisted exclusively of animal tissue had markedly lower mortality and consumed substantially more food than crabs whose diet consisted exclusively of seaweed. The quantity of food consumed had a significant positive influence on reproductive effort and long-term energy stores. Additionally, seaweed diets produced a three-fold decrease in hepatopancreas lipid content and a simultaneous two-fold increase in crab aggression when compared to an animal diet. Our results reveal that the consumption of animal tissue substantially enhanced C. sapidus fitness, and suggest that a dietary shift to plant tissue may reduce crab population growth by decreasing fecundity as well as increasing mortality. This study has implications for C. sapidus fisheries.

  9. The Influence of Diet Composition on Fitness of the Blue Crab, Callinectes sapidus.

    Science.gov (United States)

    Belgrad, Benjamin A; Griffen, Blaine D

    2016-01-01

    The physiological condition and fecundity of an organism is frequently controlled by diet. As changes in environmental conditions often cause organisms to alter their foraging behavior, a comprehensive understanding of how diet influences the fitness of an individual is central to predicting the effect of environmental change on population dynamics. We experimentally manipulated the diet of the economically and ecologically important blue crab, Callinectes sapidus, to approximate the effects of a dietary shift from primarily animal to plant tissue, a phenomenon commonly documented in crabs. Crabs whose diet consisted exclusively of animal tissue had markedly lower mortality and consumed substantially more food than crabs whose diet consisted exclusively of seaweed. The quantity of food consumed had a significant positive influence on reproductive effort and long-term energy stores. Additionally, seaweed diets produced a three-fold decrease in hepatopancreas lipid content and a simultaneous two-fold increase in crab aggression when compared to an animal diet. Our results reveal that the consumption of animal tissue substantially enhanced C. sapidus fitness, and suggest that a dietary shift to plant tissue may reduce crab population growth by decreasing fecundity as well as increasing mortality. This study has implications for C. sapidus fisheries.

  10. A new iphiculid crab (Crustacea, Brachyura, Leucosioidea) from the Middle Miocene of Austria, with notes on palaeobiogeography of Iphiculus

    Science.gov (United States)

    Hyžný, Matúš; Gross, Martin

    2017-01-01

    A new fossil species of the iphiculid genus Iphiculus Adams & White, 1849, (Crustacea, Brachyura) is described on the basis of three specimens from the Middle Miocene Florian Beds of Styria, Austria. Iphiculus eliasi sp. nov. constitutes the first European record of the genus. This occurrence represents the oldest record of Iphiculus, having implications for the palaeobiogeographic history of the family Iphiculidae. It is suggested that Iphiculus may have originated in the Western Tethys and migrated subsequently into the Indo-West Pacific. Alternatively, its current geographic restriction to the Indo-West Pacific can be a remnant of an ancient broader geographic distribution. PMID:27811673

  11. The Atlantic blue crab Callinectes sapidus in southern European coastal waters: Distribution, impact and prospective invasion management strategies.

    Science.gov (United States)

    Mancinelli, Giorgio; Chainho, Paula; Cilenti, Lucrezia; Falco, Silvia; Kapiris, Kostas; Katselis, George; Ribeiro, Filipe

    2017-06-15

    The native distribution of the blue crab Callinectes sapidus in the western Atlantic extends from Nova Scotia to Argentina. Introduced to Europe at the beginning of the 20th century, it is currently recorded almost ubiquitously in the Mediterranean and in the Black Sea. An overview of the occurrence, abundance, and ecological impact of the species in southern European waters is provided; additionally, we present a pragmatic assessment of its management scenarios, explicitly considering the dual nature of C. sapidus as both an invasive species and a fishery resource. We emphasise that the ongoing expansion of C. sapidus in the region may represent a stimulating challenge for the identification and implementation of future strategies in the management of invasive crustaceans. The impact of the invader could be converted into an enhancement of the services delivered by southern European coastal ecosystems, while mitigation costs could be transformed into profits for local populations. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Descrição de um Novo Decapoda (Natantia, Malacostraca, Crustacea

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    Márcia Aparecida Fernandes dos Reis

    2005-08-01

    Full Text Available A “lobster” fossil from the Riachuelo Formation, Sergipe Sub-basin, is here described and placed in the Decapoda, Infraorder Palinura, based on the flattened carapace and the well-developed abdomen. The specimen is characterized by a dorsal median keel and a keel in each margin of the carapace and abdomen. Field data and petrographic studies suggest that the environment in which the Decapoda lived was probably a place with waves and moderate currents, located behind a sand barrier or in within a lagoon. Apparently, the carbonization process was responsible for specimen preservation.

  13. Dimensionamento e sobreposição de nichos dos portunídeos (Decapoda, Brachyura, na Enseada da Fortaleza, Ubatuba, São Paulo, Brasil Breadth and overlap niches of the portunid crabs (Decapoda, Brachyura, in Fortaleza Bay, Ubatuba, São Paulo, Brazil

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    Marcelo Antonio Amaro Pinheiro

    1997-01-01

    Full Text Available The niche breadth of the Portunidae and their overlap on the subtidal sediments of Fortaleza bay, Ubatuba (São Paulo was analyzed. Samples were made monthly from November/1988 to October/1989, inseven areas of the bay using a shrimp fishery boat equipped with two otter-trawls. Each area was characterized based on environmental factors such as depth, temperature, salinity, dissolved oxygen, organic matter and granulometric composition of the sediment. The number of individuais of each species was registered to each area (resource. Levins's standardized measure (BA and niche percentage were calculated. Five species of swimming crabs were recorded in this study: Callinectes ornatus Ordway, 1863, Arenaeus cribrarius (Lamarck, 1818, Portunus spinimanus Latreille, 1819, Callinectes danae Smith, 1869 and Portunus spinicarpus (Stimpson, 1871. The widest ecological niche occurred to C. ornatus, present in all sampled areas (generalist species. Such fact can be related to high sediment tolerance when it is compared to P. spinimanus (especialist species which was limited to the areas with coarse granulometric fractions. Highest niche overlap was verified between C. danae and A. cribrarius may be due to greater salinity tolerance of these species. The low occurrence of P. spinicarpus and its reduced niche size in Fortaleza Bay are due to association of this species to cold water currents (ACAS more evidente in smaler depths during summer months. One future evaluation of the portunid diet can be useful to complement informations about this important aspect of the marine ecology.

  14. Reproductive biology of the sesarmid crab Armases rubripes (Decapoda, Brachyura from an estuarine area of the Sahy River, Sepetiba Bay, Rio de Janeiro, Brazil Biologia reprodutiva do caranguejo sesarmídeo Armases rubripes (Decapoda, Brachyura de uma área estuarina do rio Sahy, Baía de Sepetiba, Rio de Janeiro, Brasil

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    Giovana V. Lima

    2006-03-01

    Full Text Available The aim of this work was to gain knowledge about reproductive biology of the crab Armases rubripes (Rathbun, 1897 from an estuarine area of the Sepetiba Bay. Samples were taken monthly from February 2003 to January 2004 in the Sahy River estuary (22º56'S; 44º01'W, Rio de Janeiro, Brazil. The crabs were collected by hand during 15-minute catch-effort sessions conducted by two people. In the laboratory, the specimens were separated by sex, carapace width was measured and gonadal stage was checked macroscopically. A total of 830 individuals were caught - 304 males, 373 females (60 ovigerous females and 153 juveniles. The ovigerous females were found almost year-round, except in November and April, showing a continuous reproductive period. They presented a size range from 8.2 to 15.0 mm carapace width (12.1 ± 1.7 mm. Color and macroscopical aspects determined five gonadal stages for males and females (immature, rudimentary, intermediary, developed and resting. First sexual maturity was estimated at 6.5 mm of carapace width for males and 8.1 mm for females. Individual fecundity varied from 200 to 11,460 eggs (4,458 ± 2,739 eggs. Mean egg size was 0.248 ± 0.026 mm, varying from 0.213 to 0.333 mm, while the volume ranged from 0.0051 to 0.0188 mm³ (0.0082 ± 0.0029 mm³.O objetivo desse trabalho foi obter informações sobre a biologia reprodutiva do caranguejo Armases rubripes (Rathbun, 1897 numa área estuarina da Baía de Sepetiba. Exemplares foram obtidos mensalmente de fevereiro 2003 a janeiro 2004 no estuário do rio Sahy (22º56'S; 44º01'W, Rio de Janeiro, Brasil. Os caranguejos foram coletados manualmente durante 15 minutos por estação de coleta e por dois coletores. Em laboratório, os indivíduos foram separados por sexos, mensurados em relação à largura da carapaça e os estágios gonadais observados macroscopicamente. Um total de 830 indivíduos foram obtidos - 304 machos, 373 fêmeas (60 fêmeas ovígeras e 153 juvenis. As fêmeas ovígeras foram encontradas durante todo o período de estudo, exceto em novembro e abril, demonstrando um período reprodutivo contínuo. O tamanho das fêmeas ovígeras variou entre 8,2 e 15,0 mm de largura da carapaça (12,1 ± 1,7 mm. As gônadas observadas apresentaram cinco estágios de desenvolvimento, de acordo com a coloração e o aspecto macroscópico (imatura, rudimentar, intermediária, desenvolvida e desovada. A maturidade sexual foi estimada aos 6,5 mm de largura da carapaça entre os machos e 8,1 mm entre as fêmeas. A fecundidade individual variou entre 200 e 11.460 ovos (4.458 ± 2.739 ovos. O tamanho médio dos ovos foi de 0,248 ± 0,026 mm, variando entre 0,213 e 0,333 mm, enquanto o volume variou entre 0,0051 e 0,0188 mm³ (0,0082 ± 0,0029 mm³.

  15. Distribuição do caranguejo Hepatus pudibundus (Herbst, 1785 (Crustácea, Decapoda, Brachyura na Enseada da Fortaleza, Ubatuba (SP, Brasil Distribution of calico crab Hepatus Pudibunds (Herbst, 1785 (Crustacea, Decapoda, Brachyura in Fortaleza Bay, Ubatuba (SP, Brazil

    Directory of Open Access Journals (Sweden)

    Fernando Luis Medina Mantelatto

    1995-01-01

    Full Text Available Estudos acurados sobre a fauna macrobêntica e sua relação com o ambiente são importantes para a análise da função e estrutura de áreas litorâneas. A ñnalidade deste trabalho é caracterizar uma população do caranguejo Hepatus pudibundus na Enseada de Fortaleza, Ubatuba (SP, enfocando sua distribuição espacial, relacionado-a aos fatores físico-químicos (profundidade, temperatura, oxigênio dissolvido, salinidade, textura e teor de matéria orgânica do sedimento. Com um barco de pesca, equipado com duas redes de arrasto para camarão (tipo "otter- trawl", procedeu-se a coleta dos caranguejos, efetuada mensalmente, no período de novembro/1988 a outubro/1989, em sete radiais de 1 Km cada. Foram obtidos 405 espécimes, mostrando uma abundância heterogênea. A radial IV apresentou o menor número de indivíduos, o que pode ser explicado pelas condições físicas do sedimento (bastante compactado devido a porcentagem de silte + argila e da água (baixa salinidade. Em função dos resultados obtidos, ha fortes indícios para concluir que a Enseada da Fortaleza reúne condições favoráveis à procriação e desenvolvimento de H. pudibundus. Tais condições levam a crer que pequenas variações nos fatores bióticos e abióticos não são suficientes para alterar o padrão de distribuição desta espécie, intimamente relacionada à textura e teor de matéria orgânica do sedimento.The goal of this work is to characterize the distribution of the calico crab Hepatiis pudibundus (HERBST, 1785 in Fortaleza Bay, Ubatuba (SP, analised as a function of several environmental factors. Total of 405 specimens were collected in seven radiais in the bay. Hepatus pudibundus occurred in all radiais with heterogeneous abundance and its distribution was associated to several factors, mainly the texture and organic of sediment.

  16. Amino Acid Composition of Blue Crab (Callinectes sapidus) from the North Eastern Mediterranean Sea

    OpenAIRE

    KÜÇÜKGÜLMEZ, Aygül; ÇELIK, Mehmet

    2008-01-01

    The amino acid composition in tissues (claw, breast meats and hepatopancreas) of the blue crabs (Callinectes sapidus, RATHBUN, 1896), caught off the North Eastern Mediterranean Sea (Turkey) were investigated. The amounts of the amino acid contents of the claw, breast meat and hepatopancreas of the blue crab were found to be significantly different. Glutamic acid was major amino acid in all parts of the blue crab. The total essential amino acids ranged from 7.24 to 7.83 g/ 100 g fresh wt. The ...

  17. Predation of the ribbed mussel geukensia demissa by the blue crab callinectes sapidus

    Science.gov (United States)

    Seed, R.

    Callinectes sapidus fed extensively on Geukensia demissa in laboratory aquaria. Several predation techniques used by Callinectes to open its prey are reported. Prey value decreases monotonically with increasing mussel size. Crabs consumed mussels over a wide size range but were generally reluctant to feed on larger mussels whilst smaller, more profitably prey was available. The relative importance of 'energy maximization' and 'time minimization' could not be distinguished. The distribution and population structure of Geukensia at Beaufort, N. Carolina are briefly considered in terms of the foraging strategy of Callinectes.

  18. First occurrence of Callinectes sapidus (Rathbun, 1896) within the Sacca di Goro (Italy) and surroundings

    OpenAIRE

    Manfrin, Chiara; Turolla, Edoardo; Chung, J.; Giulianini, Piero

    2015-01-01

    The Sacca di Goro lagoon is an area located in the northern part of Italy. This locality has benefited in the past of an occasional and later the planned introduction of the Manila clam. Nowadays it produces more than 50% of the entire Italian clam production. Recently the crab Callinectes sapidus has been spotted and reported a few months ago on its female carrying eggs. This may signify the complete acclimation of this species in the Sacca di Goro lagoon and a potential ongoing spread to su...

  19. Optics and phylogeny: is there an insight? The evolution of superposition eyes in the Decapoda (Crustacea)

    NARCIS (Netherlands)

    Gaten, Edward

    1998-01-01

    This paper addresses the use of eye structure and optics in the construction of crustacean phylogenies and presents an hypothesis for the evolution of superposition eyes in the Decapoda, based on the distribution of eye types in extant decapod families. It is suggested that reflecting superposition

  20. Shallow-water anomuran and brachyuran crabs (Crustacea: Decapoda from southern Bahia, Brazi l Cangrejos anomuros y braquiuros (Crustacea: Decapoda de aguas someras del sur de Bahia, Brasil

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    Alexandre O Almeida

    2010-01-01

    Full Text Available The objective of this study was to determine the taxonomic composition and ecological aspects of decapods crustacean species belonging to the infraorders Anomura and Brachyura in shallow marine and estuarine waters from southern Bahia, a coastline about 640 km in extent, corresponding to approximately 7% of the Brazilian coast. Sixteen species of the infraorder Anomura and 68 of the infraorder Brachyura are reported for the study area. The most important families in terms of number of species were the Panopeidae with 11 species, and the Ocypodidae and Portunidae with 9. Among the Brachyura, the southern distribution of the species Austinixa leptodactyla Coelho, 1997 (Pinnotheridae, endemic to Brazil, is extended from the coast of Sergipe to Bahía (Prado, Cumuruxatiba Beach, 17°06'18.6"S, 39°10'50.4"W. The ocypodid Uca (Leptuca cumulanta Crane, 1943 and also the pinnotherids Austinixa aidae (Righi, 1967 and Fabia byssomiae (Say, 1818 are reported for the first time from the Bahia coast. The specimen of F. byssomiae examined was collected in the mantle cavity of the clam Macoma constrict (Bruchiére, 1792 (Bivalvia: Tellinidae, a new host record for the species.El objetivo del presente estudio fue determinar la composición taxonómica y aspectos ecológicos de los crustáceos decápodos pertenecientes a los infraórdenes Anomura y Brachyura, en aguas someras, marinas y estuarinas del sur de Bahia, Brasil, una línea costera con cerca de 640 km de extensión, que corresponde aproximadamente al 7% de la costa brasileña. Para esta area de estudio se registraron 16 especies del infraorden Anomura y 68 del infraorden Brachyura. Las familias más representativas en términos de número de especies fueron Panopeidae, con 11 especies, y Ocypodidae y Portunidae, ambas con nueve especies. Entre los Brachyura, la distribución meridional de Austinixa leptodactyla Coelho, 1997 (Pinnotheridae, endémica del Brasil, se extiende desde la costa de Sergipe

  1. Local distribution and abundance of Cardisoma guanhumi Latreille, 1928 (Brachyura: Gecarcinidae in southern Brazil

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    JF Oliveira-Neto

    Full Text Available The blue land crab, Cardisoma guanhumi Latreille, 1828 (Brachyura: Gecarcinidae is officially included in the list of over-exploited species in Brazil, although still abundantly found in the state of Santa Catarina, the southern limit of its distribution. This species was found in forested areas, gardens, and grassy areas, including crabs with carapace width larger than 80mm. The existence of this population with these characteristics is surprising, since there is only one official record of the species in the southern region. The objectives of this study are to estimate the abundance and occupation patterns of C. guanhumi in this region. Correlations with conservation were discussed. The absolute abundance of crabs in the middle of summer activity was established for an area of 100,000 m2. A smaller area was mapped and divided into sampling units for statistical analyses. We distributed approximately 240 crabs in a forested area of about 3,000 m2 and 150 crabs in grassy areas (90,000 m2. The statistical test of Kruskal-Wallis test showed that there are significant differences between the sizes of the openings of the galleries inside the forest and that located in grassy areas. In the forest, the openings tend to be much larger. Burrows were found at a distance of 150 metres from the channel. The number of galleries was higher in the forested area, although the burrows were more densely grouped in grassy areas. Although C. guanhumi seems to be adjusting well to changes caused by human occupation, small forested areas are more conducive to growth and conservation of this species.

  2. Effects of molting on the visual acuity of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Baldwin, Jamie; Johnsen, Sönke

    2011-09-15

    In crustaceans with compound eyes, the corneal lens of each facet is part of the exoskeleton and thus shed during molting. Here we used an optomotor assay to evaluate the impact of molting on visual acuity (as measured by the minimum resolvable angle, α(min)) in the female blue crab, Callinectes sapidus. We found that visual acuity decreases substantially in the days prior to molting and is gradually recovered after molting. Four days prior to molting, α(min) was 1.8 deg (N=5), a value approximating the best possible acuity in this species. In the 24 h before ecdysis occurred, α(min) increased to 15.0 deg (N=12), corresponding to an eightfold drop in visual acuity. Within 6 days after molting, α(min) returned to the pre-molting value. Micrographs of C. sapidus eyes showed that a gap between the corneal lens and the crystalline cone first appeared approximately 5 days prior to shedding and increased in width as the process progressed. This separation was likely responsible for the loss of visual acuity observed in behavioral tests. In blue crabs, mating is limited to the period of the female's pubertal molt, and a reduction in acuity during this time may have an effect on the sensory cues used in female mate choice. The results described here may be broadly applicable to all arthropods that molt and have particular importance for crustaceans that molt multiple times in their lifetime or have mating cycles that are paired with molting.

  3. The importance of color in mate choice of the blue crab Callinectes sapidus.

    Science.gov (United States)

    Baldwin, Jamie; Johnsen, Sönke

    2009-11-01

    Visual displays often play a large role in animal communication, particularly in sexual interactions. The blue crab Callinectes sapidus is both colorful and highly visually responsive, yet almost all studies of their courtship have focused on chemical cues. In the blue crab's underwater environment, however, visual cues may function more rapidly and over a longer distance than chemical cues. Given that blue crabs are aggressive and cannibalistic, visual cues may therefore allow blue crabs to quickly evaluate potential mates from safer distances. In the present study we show that courtship and mate choice behavior in C. sapidus can be stimulated by visual cues alone. Further, we show that males have a preference for females with red claw dactyls. In binary choice experiments, males displayed more often to photographs of females with red claws than to those with white claws or to those with black claws that were isoluminant to the red ones. This strongly suggests that male blue crabs made their choices based on the hue of the red claws, further suggesting that blue crabs are capable of color vision and use color in mate choice.

  4. Properties of bacteria that trigger hemocytopenia in the Atlantic blue crab, Callinectes sapidus.

    Science.gov (United States)

    Johnson, Nathaniel G; Burnett, Louis E; Burnett, Karen G

    2011-10-01

    In the blue crab Callinectes sapidus, injection with the bacterial pathogen Vibrio campbellii causes a decrease in oxygen consumption. Histological and physiological evidence suggests that the physical obstruction of hemolymph flow through the gill vasculature, caused by aggregations of bacteria and hemocytes, underlies the decrease in aerobic function associated with bacterial infection. We sought to elucidate the bacterial properties sufficient to induce a decrease in circulating hemocytes (hemocytopenia) as an indicator for the initiation of hemocyte aggregation and subsequent impairment of respiration. Lipopolysaccharide (LPS), the primary component of the gram-negative bacterial cell wall, is known to interact with crustacean hemocytes. Purified LPS was covalently bound to the surfaces of polystyrene beads resembling bacteria in size. Injection of these "LPS beads" caused a decrease in circulating hemocytes comparable to that seen with V. campbellii injection, while beads alone failed to do so. These data suggest that in general, gram-negative bacteria could stimulate hemocytopenia. To test this hypothesis, crabs were injected with different bacteria--seven gram-negative and one gram-positive species--and their effects on circulating hemocytes were assessed. With one exception, all gram-negative strains caused decreases in circulating hemocytes, suggesting an important role for LPS in the induction of this response. However, LPS is not necessary to provoke the immune response given that Bacillus coral, a gram-positive species that lacks LPS, caused a decrease in circulating hemocytes. These results suggest that a wide range of bacteria could impair metabolism in C. sapidus.

  5. Effects of soluble fractions of drilling fluids on development of crabs, Rhithropanopeus harrisii and Callinectes sapidus

    Energy Technology Data Exchange (ETDEWEB)

    Bookhout, C.G.; Monroe, R.J.; Forward, R.B. Jr.; Costlow, J.D. Jr.

    1984-01-01

    The mud aqueous fraction (MAF) and suspended particulate phase (SPP) of low-density lignosulfonate type mud with ferrochrome added were nontoxic to larvae during the complete larval development of Rhithropanopeus harrisii. Five percent (5000 ppm, 0.5 v/v mud in water) MAF and SPP were not toxic to Callinectes sapidus. Survival of C. sapidus larvae decreased as concentrations of MAF and SPP increased from 5% (5000 ppm, 0.5 v/v mud in water) to 50% (50,000 ppm, 5% v/v mud in water). No larvae reached the 1st crab stage in 100% (100,000 ppm, 10% v/v mud in water) MAF and SPP. Statistical analysis of the data on survival, mortality, and behavior are presented. Blue crab larval behavior is affected by exposure to MAF and SPP with the general effect being a decline in swimming speed. A significant reduction was only observed in 100% MAF but was noticed in 5, 25, 50, and 100% SPP.

  6. Crecimiento del cangrejo Callinectes danae (Decapoda: Portunidae de la Isla de Margarita, Venezuela

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    Jesylén Castillo

    2011-12-01

    Full Text Available Las especies del género Callinectes sostienen pesquerías importantes en varias partes del mundo. En el occidente de Venezuela, Callinectes sapidus es capturado con palangre y sostiene una pesquería artesanal importante en el Lago de Maracaibo; mientras que en la Isla de Margarita, Callinectes danae es capturado con nasa en zonas cercanas a lagunas costeras y su pesca está limitada por la oferta y la demanda. Estos recursos son de considerable importancia económica entre los crustáceos comestibles; sin embargo, pocos estudios han sido reportados sobre la dinámica poblacional y potencialidad pesquera en ambientes costeros de Venezuela que contribuyan a la toma de decisiones en la administración pesquera. En este documento se presenta información de los parámetros de crecimiento del cangrejo Callinectes danae Smith, 1869, con el fin de obtener un conocimiento detallado del patrón de crecimiento y lograr estimaciones más precisas sobre el tamaño de la población y su disponibilidad para la explotación. Se analizó una muestra constituida por 3 623 ejemplares capturados con nasa cangrejera por la flota artesanal, entre octubre 2007 y septiembre 2008. Se estableció la relación longitud-peso y se estimaron los parámetros de crecimiento de las curvas en longitud y peso del modelo de von Bertalanffy. La proporción sexual global mostró que no existe diferencia significativa entre machos y hembras (χ2=0.04, p>0.05. Los valores de las pendientes b entre machos y hembras presentaron diferencias significativas (ts=2.75, pDana Swimming crab growth Callinectes danae (Decapoda: Portunidae from Margarita Island, Venezuela. Callinectes danae is a common species captured with crab traps in nearby areas of coastal lagoons in Margarita Island. Although its considerable economic importance as a fishery resource, few studies have been done on population dynamics and its fishery potential in local coastal environments to support decision making in

  7. Concentration of HCB in Callinectes sapidus from the Calcasieu Estuary, Louisiana

    Energy Technology Data Exchange (ETDEWEB)

    Murray, H.E.; Murphy, C.N.; Gaston, G.R. (McNeese State Univ., Lake Charles, LA (United States))

    1992-01-01

    Blue crabs (Callinectes sapidus) were collected from several areas in the estuary, and edible tissues were analyzed for the organic compound hexachlorobenzene (HCB). Hexachlorobenzene was established as an indicator of industrial chemical contamination by the Louisiana Department of Health and Hospitals (LDHH). This organization used the FDA interim guideline concentration of 0.5 ppm HCB in fatty tissue of beef to develop an emergency action level of 0.060 ppm for edible seafood. In this study, an average HCB tissue concentration of 40 ppb was found in the Calcasieu Estuary. There was no significant difference in concentrations between sampling locations. However, the range in HCB concentrations varied among samples, with some analyses yielding tissue concentrations greater than the emergency action level of 60 ppb.

  8. Toxicity of the dispersant Corexit 9500 to early life stages of blue crab, Callinectes sapidus.

    Science.gov (United States)

    Anderson Lively, Julie A; McKenzie, Jon

    2014-12-01

    The Deepwater Horizon well released 4.4 million barrels of light crude oil offshore of Louisiana into one of the world's largest and most productive blue crab (Callinectes sapidus) fisheries. The objectives of this paper were to determine the toxicity of the dispersant Corexit(®) 9500A used in the 2010 oil spill on juvenile and larval blue crabs, and the long-term effects of sublethal acute exposure. Only the highest treatment levels of dispersant significantly increased mortality in larval and juvenile blue crabs (100 mg/L and 1,000 mg/L, respectively). This correlated to concentrations well above levels found in the Gulf of Mexico following the spill. Smaller and younger crabs showed higher mortality than older and larger crabs. This research indicates direct application of dispersants on crab larvae could cause acute mortality, but dilution through diffusion and natural weathering processes would minimize long-term effects.

  9. Cadmium-binding proteins from blue crabs (Callinectes sapidus) environmentally exposed to cadmium

    Energy Technology Data Exchange (ETDEWEB)

    Wiedow, M.A.; Kneip, T.J.; Garte, S.J.

    1982-06-01

    Two heat-stable (90/sup 0/C) cadmium-binding proteins were isolated from the hepatopancreas of Hudson River blue crabs (Callinectes sapidus) by Sephadex G-75 gel filtration chromatography. These proteins have molecular weights of 10,600 and 9,400, and ultraviolet absorbance ratios at 250/280 nm of 12.4 and 5.4, respectively. Repeated freezing and thawing and prolonged (3-6 weeks) storage resulted in protein degradation or loss of Cd-binding activity. These proteins were induced by laboratory injection of CdCl/sub 2/ in blue crabs from pristine (Chesapeake Bay) areas; however, injection of CdCl/sub 2/ into Hudson River animals yielded anomalous chromatography profiles. Cadmium-binding proteins were also identified in blue crab thoracic muscle and gill. The possibility is discussed that these proteins are a type of metallothionein and could contribute to the human toxicity of this cadmium-contaminated edible crustacean.

  10. Heavy Metals in the Blue Crab (Callinectes sapidus) in Mersin Bay, Turkey.

    Science.gov (United States)

    Çoğun, H Y; Firat, Ö; Aytekin, T; Firidin, G; Firat, Ö; Varkal, H; Temiz, Ö; Kargin, F

    2017-06-01

    The concentrations of four metals (copper, cadmium, zinc and lead) were quantified in blue crabs (Callinectes sapidus) tissues collected in January, April, June and September at two stations in Mersin Bay, northeastern Mediterranean Sea, using ICP-MS. The metal concentrations in crabs from the two stations differed significantly. The hepatopancreas was the major organ accumulating metals, followed by gill and muscle tissues. Except for lead, the highest concentrations of metals were measured in the hepatopancreas. The hepatopancreas had higher concentrations of all heavy metals (Cu 321.1 ± 4.30; Zn 182.2 ± 3.40; Cd 48.2 ± 2.00) compared to gill (Cu 90.2 ± 1.35; Zn 104.3 ± 2.30; Cd 22.3 ± 3.40) and muscle (Cu 19.1 ± 1.10; Zn 55.1 ± 3.25; Cd 2.5 ± 0.20). Among the metals analyzed, Cu, Zn and Pb were the most abundant in the different tissues while Cd was the least abundant in C. sapidus. Seasonality in the levels of the four metals was determined.The highest concentrations of all metals were observed in the June (Zn 55.1 ± 3.25; Cu 19.1 ± 1.10; Cd 2.5 ± 0.20; Pb 5.1 ± 0.18) compared to all other months (Zn 34.1 ± 3.23; Cu 11.1 ± 1.15; Cd 0.9 ± 0.20; Pb 3.1 ± 0.21).

  11. Metallothionein-like proteins in the blue crab Callinectes sapidus: effect of water salinity and ions.

    Science.gov (United States)

    De Martinez Gaspar Martins, Camila; Bianchini, Adalto

    2009-03-01

    The effect of water salinity and ions on metallothionein-like proteins (MTLP) concentration was evaluated in the blue crab Callinectes sapidus. MTLP concentration was measured in tissues (hepatopancreas and gills) of crabs acclimated to salinity 30 ppt and abruptly subjected to a hypo-osmotic shock (salinity 2 ppt). It was also measured in isolated gills (anterior and posterior) of crabs acclimated to salinity 30 ppt. Gills were perfused with and incubated in an isosmotic saline solution (ISS) or perfused with ISS and incubated in a hypo-osmotic saline solution (HSS). The effect of each single water ion on gill MTLP concentration was also analyzed in isolated and perfused gills through experiments of ion substitution in the incubation medium. In vivo, MTLP concentration was higher in hepatopancreas than in gills, being not affected by the hypo-osmotic shock. However, MTLP concentration in posterior and anterior gills significantly increased after 2 and 24 h of hypo-osmotic shock, respectively. In vitro, it was also increased when anterior and posterior gills were perfused with ISS and incubated in HSS. In isolated and perfused posterior gills, MTLP concentration was inversely correlated with the calcium concentration in the ISS used to incubate gills. Together, these findings indicate that an increased gill MTLP concentration in low salinity is an adaptive response of the blue crab C. sapidus to the hypo-osmotic stress. This response is mediated, at least in part, by the calcium concentration in the gill bath medium. The data also suggest that the trigger for this increase is purely branchial and not systemic.

  12. Silicification of the medial tooth in the blue crab Callinectes sapidus.

    Science.gov (United States)

    Nesbit, Katherine T; Roer, Robert D

    2016-12-01

    Observations of cuticular structures mineralized with silica within the Crustacea have been limited to the opal teeth of copepods, mandibles of amphipods, and recently the teeth of the gastric mill in the blue crab Callinectes sapidus. Copepod teeth are deposited during premolt, with sequential elaboration of organic materials followed by secretion of silica into the tooth mold. The timing of mineralization is in stark contrast to that of the general integument of crustaceans in which calcification is completely restricted to the postmolt period. To determine the timing of molt-related deposition and silicification of the teeth of the gastric mill, the medial tooth of the blue crab C. sapidus was examined histologically and ultrastructurally across the molt cycle. Histological data revealed deposition of the organic matrix of the epicuticle and exocuticle during premolt. No evidence of postmolt changes in the thickness of the epicuticle and exocuticle, or any deposition of endocuticle, was observed. Scanning electron microscopy revealed degradation of the outer surface of the old tooth during premolt. During premolt, epithelial structures resembling papilla appeared to secrete a fibrous web that coalesces to become the matrix of the new tooth. Semi-quantitative elemental analyses indicated simultaneous deposition of silica and organic matrix, and demonstrated a homogeneous distribution of silicon throughout the epicuticle of the tooth at all stages. However, there is evidence of deposition (presumably silicification) during postmolt as spaces between the papillae become filled in. Thus, the pattern and timing of deposition and silicification of the tooth are different from both teeth of copepods and the general exoskeleton of decapods, and may facilitate rapid resumption of feeding and consumption of the exuvia in early postmolt. J. Morphol. 277:1648-1660, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  13. Respiratory and digestive responses of postprandial Dungeness crabs, Cancer magister, and blue crabs, Callinectes sapidus, during hyposaline exposure.

    Science.gov (United States)

    Curtis, Daniel L; McGaw, Iain J

    2010-02-01

    Respiratory responses and gastric processing were examined during hyposaline exposure in two crab species of differing osmoregulatory ability. The efficient osmoregulator, Callinectes sapidus, displayed an immediate increase in oxygen uptake when exposed to low salinity in isolation. In contrast, the weak osmoregulator, Cancer magister, showed no change in oxygen uptake upon acute exposure (sapidus were able to sum the demands of osmoregulation and digestion. Thus, gastric processes continued unabated in low salinity. Conversely, postprandial C. magister prioritized responses to low salinity over those of digestion, resulting in a decrease in oxygen uptake when exposed to low salinity. This decrease in oxygen uptake corresponded to a reduction in the rate of contraction of the pyloric stomach and a subsequent doubling of gastric evacuation time. The current study is one of the few to illustrate how summation or prioritization of competing physiological systems is manifested in digestive processes.

  14. Detailed Spectroscopic Study of the Role of Br and Sr in Coloured Parts of the Callinectes Sapidus Crab Claw

    OpenAIRE

    Katsikini, Maria

    2016-01-01

    The exoskeleton of crustaceans consists mainly of calcium carbonate (CaCO$_3$) minerals and in many cases exhibits vivid colouration due to the presence of proteins rich in carotenoid chromophores. The exposure of aquatic animals in sea water results often in the incorporation of trace elements in their exoskeleton. The bonding configuration of Br and Sr trace elements in regions with different staining (white, orange and blue) of the exoskeleton of the Callinectes sapidus in crab claw are sy...

  15. New records of Decapod Crustaceans (Decapoda: Pontoniinae and Inachidae associated with sea anemones in Turkish waters

    Directory of Open Access Journals (Sweden)

    Z. DURIS

    2013-10-01

    Full Text Available Three anemone-associated decapod crustaceans, two shrimp species, Periclimenes amethysteus and P. aegylios (Caridea: Palaemonidae: Pontoniinae, and the crab Inachus phalangium (Brachyura: Inachidae, all collected from the Dardanelles, are reported for the first time from Turkish coasts. Another inachid crab, Macropodia czernjawskii is also reported for the first time to occur in association with the sea anemone, Anemonia viridis. Periclimenes scriptus was the fifth decapod species recorded associated with sea anemones within the present study, and while this species has already been reported from Turkish waters, this is the first time it is recorded from the Dardanelles (the Turkish Straits System.

  16. New primers for amplification of cytochrome c oxidase subunit I barcode region designed for species of Decapoda (Crustacea)

    OpenAIRE

    Fernando L. Mantelatto; Carvalho, Fabrício L.; Simões,Sabrina M.; Mariana Negri; Souza-Carvalho,Edvanda A.; Mariana Terossi

    2016-01-01

    Abstract We designed 14 new primers for amplification of the COI barcode region of decapod crustacean species. We tested, with high level of success, the generation of ~ 640 ± 49 base-pair sequences in selected groups of decapods (hermit crabs, squat lobsters, marine and freshwater crabs and shrimps), encompassing representatives of 27 genera of 15 families, 11 of Pleocyemata (Anomura, Brachyura, and Caridea) and 4 of Dendrobranchiata. Based on the results we expect the applicability of these...

  17. Antioxidant potential analysis of P. ostreatus, P. sajor-caju, P. sapidus and P. columbinus.

    Science.gov (United States)

    Irshad, A; Shahid, M; Asghar, M; Khan, J A

    2017-01-01

    The present study was conducted to evaluate the antioxidant activity of ethanol, n-hexane, dichloromethane, ethyl acetate and water extracts of four different Pleurotus spps. P. ostreatus, P. sajor-caju, P. sapidus and P. columbinus. The extraction was performed by classical organic solvent extraction (COSE). The extracts of Pleurotus spps. contained appreciable levels of total phenolic contents (TPC) (0.95-19.49 GAE, mg/g) and total flavonoid contents (0.85-3.73 CE, mg/g). All Pleurotus spps. also contained considerable DPPH radical scavenging activity, showing IC50 (19.15-54.50%) and reducing power (0.50-2.94 nm), respectively. The ascorbic acid content was in the range of (2.90-5.97 mg/g) for all Pleurotus spps. All studied Pleurotus spps. showed potential antioxidant activity. The results of four different Pleurotus spps. extracts showed that they can be used as a good food ingredient, and as a medicinal mushroom for digestive ailments, as well as in the pharmaceutical industry and in cosmetics.

  18. Chelal Characteristics and Foraging Behaviour of the Blue Crab Callinectes sapidusRathbun

    Science.gov (United States)

    Seed, R.; Hughes, R. N.

    1997-02-01

    This paper describes the morphological and mechanical characteristics of the chelae of Callinectes sapidus, together with the predation techniques, handling times and prey size selection of this voracious predator when presented with marsh mussels, Geukensia demissa, or fiddler crabs, Uca pugilator, in laboratory aquaria. Callinectes sapidusis heterochelous but differences in claw size and mechanical advantage between male and female crabs are statistically indistinguishable. Handling times for mussels and fiddler crabs increased steeply with prey size. Fiddler crabs are intrinsically more profitable than mussels, but the relative costs of capturing mobile prey, or excavating mussels, in natural populations is unknown. Previous laboratory experiments investigating the key stimuli used by C. sapiduswhen selecting prey are reviewed, and their results are compared with behaviour predicted by optimal foraging theory. It is concluded that crabs respond to the strongest stimuli received from the prey. Items were rejected after a given persistence time if the attack proved unsuccessful. Persistence time, however, was adjusted through experience to expectations of local prey quality and availability. The integrated result of this key-stimulus mechanism will be a close match to diets predicted by other behavioural models, including that based on optimal foraging theory.

  19. Evaluation of Blue Crab, Callinectes sapidus, Megalopal Settlement and Condition during the Deepwater Horizon Oil Spill

    Science.gov (United States)

    Grey, Erin K.; Chiasson, Susan C.; Williams, Hannah G.; Troeger, Victoria J.; Taylor, Caz M.

    2015-01-01

    The Blue Crab, Callinectes sapidus, is a commercially, culturally, and ecologically significant species in the Gulf of Mexico (GOM), whose offshore stages were likely impacted by the Deepwater Horizon oil spill (DWH). To test for DWH effects and to better understand the planktonic ecology of this species, we monitored Callinectes spp. megalopal settlement and condition at sites within and outside of the spill extent during and one year after the DWH. We tested for DWH effects by comparing 2010 settlement against baseline data available for two sites, and by testing for differences in settlement and condition inside and outside of the spill extent. We also developed time series models to better understand natural drivers of daily settlement variation (seasonal and lunar trends, hydrodynamics, wind) during 2010 and 2011. Overall, we found that neither megalopal settlement nor body weight were significantly reduced at oiled sites, but that high unexplained variation and low statistical power made detection of even large effects unlikely. Time series models revealed remarkably consistent and relatively strong seasonal and lunar trends within sites (explaining on average 28% and 9% of variation, respectively), while wind and hydrodynamic effects were weak (1–5% variation explained) and variable among sites. This study provides insights into DWH impacts as well as the natural drivers of Callinectes spp. megalopal settlement across the northern GOM. PMID:26270970

  20. Discovery of an opportunistic starfish pathogen, Orchitophrya stellarum, in captive blue crabs, Callinectes sapidus.

    Science.gov (United States)

    Small, H J; Miller, T L; Coffey, A H; Delaney, K L; Schott, E; Shields, J D

    2013-10-01

    Histophagous scuticociliate infections were discovered in blue crabs, Callinectes sapidus, held in research facilities at the Virginia Institute of Marine Science. Ciliates were observed infecting every tissue examined including the gills, heart, muscle, hepatopancreas, and epidermis. Hemolymph smears and histological tissue sections indicated a morphological similarity to Mesanophrys chesapeakensis, the only recorded histophagous ciliate infecting blue crabs. However, subsequent analysis of the ribosomal ITS region (ITS1-5.8S-ITS2) of the ciliate indicated the parasite was Orchitophrya stellarum, a parasitic ciliate previously reported infecting sea stars from Europe, Australia, and North America. A simple Polymerase Chain Reaction (PCR-RFLP) assay was developed to detect and differentiate between O. stellarum and M. chesapeakensis. Its application confirmed the presence of O. stellarum infecting blue crabs held in an additional research facility in Maryland. For growth studies, cultures of O. stellarum grew optimally on 10% blue crab serum in crustacean saline held at 10-20°C. A field survey of blue crabs collected during the winters of 2011-2012 and sea stars (Asterias forbesi) during the winter of 2010 from the Chesapeake Bay and eastern shore of Virginia did not identify additional infected individuals. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. The Effects of Ocean Acidification on Predator-Prey Interactions between Mya arenaria and Callinectes sapidus

    Science.gov (United States)

    Longmire, K.; Glaspie, C.; Seitz, R.

    2016-02-01

    The study examined the implications of ocean acidification for Mya arenaria and the predator-prey dynamics between M. arenaria and Callinectes sapidus. Clams were subjected to either ambient conditions or acidified conditions and grown over four weeks. Mortality, shell lengths, and biomass (ash-free dry weights) were recorded for clams destructively sampled each week. Clams were subjected to behavioral experiments to determine their response to an approaching physical disturbance. Crabs were exposed to acidified or ambient conditions for 48 hours, and placed in 48 hour mesocosm trials with clams. Shell lengths, mortality and biomass between the ambient and acidified clams were not significantly different between acidified and ambient treatments. Shell ash weights were lower for acidified clams, evidence of shell dissolution. In the behavioral experiment, ocean acidification reduced the ability of clams to respond to a predator stimulus. Lastly, in predator-prey mesocosm trials, in ambient conditions, crabs ate all or none of the available clams, whereas acidified crabs ate all available clams in many trials and ate at least one acidified clam per trial. The early effects of ocean acidification on M. arenaria will manifest in trophic interactions with other species, rather than impacting M. arenaria alone.

  2. A Predictive Model for the Growth of Listeria monocytogenes in Commercial Blue Crab (Callinectes sapidus).

    Science.gov (United States)

    Parveen, Salina; White, Channel; Tamplin, Mark L

    2017-10-13

    During the processing and handling of commercial blue crab (Callinectes sapidus), Listeria monocytogenes can potentially contaminate cooked meat and grow to hazardous levels. To manage this risk, predictive models are useful tools for designing and implementing preventive controls; however, no model specific for blue crab meat has been published or evaluated. In this study, a cocktail of L. monocytogenes strains was added to pasteurized blue crab meat, which was incubated at storage temperatures from 0 to 35°C. At selected time intervals, L. monocytogenes was enumerated by direct plating onto modified Oxford agar. A primary model was fitted to kinetic data to estimate the lag-phase duration (LPD) and growth rate (GR). Listeria monocytogenes replicated from 0 to 35°C, with GR ranging from 0.004 to 0.518 log CFU/h. Overall, the LPD decreased with increasing temperature, displaying a maximum value of 187 h at 0°C; however, this trend was not consistent. The LPD was not detected at 10°C, and it occurred inconsistently from trial to trial. A secondary GR model (R 2 = 0.9892) for pasteurized crab meat was compared with the L. monocytogenes GR in fresh crab meat, demonstrating bias and accuracy factors of 0.98 and 1.36, respectively. The model estimates varied from other published data and models, especially at temperatures ≥5°C, supporting the need for a specific predictive tool for temperature deviations.

  3. High Connectivity among Blue Crab (Callinectes sapidus) Populations in the Western South Atlantic.

    Science.gov (United States)

    Lacerda, Ana Luzia Figueiredo; Kersanach, Ralf; Cortinhas, Maria Cristina Silva; Prata, Pedro Fernandes Sanmartin; Dumont, Luiz Felipe Cestari; Proietti, Maíra Carneiro; Maggioni, Rodrigo; D'Incao, Fernando

    2016-01-01

    Population connectivity in the blue crab Callinectes sapidus was evaluated along 740 km of the Western South Atlantic coast. Blue crabs are the most exploited portunid in Brazil. Despite their economic importance, few studies report their ecology or population structure. Here we sampled four estuarine areas in southern Brazil during winter 2013 and summer 2014 in order to evaluate diversity, gene flow and structure of these populations. Nine microsatellite markers were evaluated for 213 adult crabs, with identification of seven polymorphic loci and 183 alleles. Pairwise FST values indicated low population structure ranging from -0.00023 to 0.01755. A Mantel test revealed that the geographic distance does not influence genetic (r = -0.48), and structure/migration rates confirmed this, showing that even the populations located at the opposite extremities of our covered region presented low FST and exchanged migrants. These findings show that there is a significant amount of gene flow between blue crab populations in South Brazil, likely influenced by local current dynamics that allow the transport of a high number of larvae between estuaries. Considering the elevated gene flow, the populations can be considered a single genetic stock. However, further information on population size and dynamics, as well as fishery demands and impacts at different regions, are necessary for harvest management purposes.

  4. Incidence of bacteremia in stressed and unstressed populations of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Welsh, P C; Sizemore, R K

    1985-01-01

    The incidence of bacteremia in the blue crab, Callinectes sapidus, is reported to be in excess of 80%. Because these results have been controversial, a field study was initiated to determine the effect of commercial capture and handling stresses on the incidence and levels of infection in blue crabs. The majority (75%) of "unstressed" crabs which were captured individually and bled immediately upon removal from the water were bacteremic, with a geometric mean level of infection of 14 CFU/ml of hemolymph. Crabs collected by crab pot, confined within these pots for as long as 24 h, and sampled immediately after removal from the water had a similar mean level of infection. Crabs subjected to the stresses of commercial capture, handling, and transport showed a higher incidence of infection (91%) and a mean infection level of 46 CFU/ml. Injuries sustained by crabs during commercial handling are thought to be associated with the higher incidence of infection. Vibrio spp. were primarily responsible for progressive infections in commercially stressed crabs and were the predominant bacterial type in heavily infected crabs. Our results indicated that uninjured healthy crabs do not have sterile hemolymph but instead harbor low-level bacterial infections. PMID:4051486

  5. Effects of cadmium on survival and bioenergetics of juvenile Callinectes sapidus at different salinities

    Energy Technology Data Exchange (ETDEWEB)

    Guerin, J.L. [Environmental Protection Agency, Gulf Breeze, FL (United States). Gulf Breeze Environmental Research Lab.; Stickle, W.B. [Louisiana State Univ., Baton Rouge, LA (United States). Dept. of Zoology and Physiology

    1995-12-31

    Juvenile blue crabs, Callinectes sapidus, from the Barataria estuary (Louisiana, USA) were exposed to a range of dissolved cadmium concentrations at 2.5 and 25{degree}/ooS to determine their response to cadmium. 21-day LC{sub 50}`s were an order of magnitude lower at 2.5{degree}/oo (19ppb) than at 25{degree}/oo (186ppb). Crabs were exposed to Cd levels of 0, 50 and 100ppb for measurements of physiological rates. Respiration was the primary component (x = 86.2%) of the energy expenditure budget in all cases, relative to excretion. Rates of energy expenditure did not vary with cadmium concentration at either salinity and were much lower than those of energy consumption and absorption. Scope for growth paralleled energy consumption and absorption. Scope for growth did not vary as a function of cadmium concentration at 25{degree}/ooS; at 2.5{degree}/ooS, however, scope for growth was significantly lower in crabs exposed to 50 and 100ppb Cd relative to control crabs. Cadmium levels of up to 100ppb do not stress juvenile blue crabs at 25{degree}/ooS; but at 2.5{degree}/ooS, these same levels of Cd result in mortality and sub-lethal stress. This finding is important since nursery grounds for juveniles of this species often lie in low salinity, estuarine waters, and dissolved cadmium levels as high as 50ppb have been reported in Louisiana estuaries.

  6. Accumulation of toxic organic pollutants in the blue crab Callinectes sapidus

    Energy Technology Data Exchange (ETDEWEB)

    Hale, R.C.

    1983-01-01

    Levels of toxic organic pollutants (TOP) were examined in the blue crabs, Callinectes sapidus, of Virginia waters. Alkyl substituted polynuclear aromatic hydrocarbons (ASPAH), thought derived from weathered petroleum, were the most common organic pollutants present. Unsubstituted aromatics, heterosubstituted aromatics, polychlorinated biphenyls (PCB), and DDT metabolites were also detected by the analytical procedure employed. Highest levels of ASPAH were found in crabs from the heavily industrialized Hampton Roads area of the James River. Lower concentrations were detected in crabs from the Pocomoke Sound, the upper James and the Rappahannock River sampling sites. PCB levels were elevated in samples taken from both James River stations. Levels of TOP were generally higher in crabs sampled in June than in September of 1981. Concentrations of pollutants in the tissues and tissue lipid content were correlated. Highest levels of both were detected in the hepatopancreas, followed by egg, ovary and muscle. TOP levels in field samples in in-vivo biotransformation of the model compound benzo(a)pyrene (B(a)P) in the hepatopancreas, during in-laboratory experiments, suggested that differences may exist in the uptake and elimination of pollutants as a function of the sex and maturity of the crabs. It appeared that the metabolism of aromatic hydrocarbons varied with the molt stage of the crabs.

  7. Comparison of metal concentrations in tissues of blue crab, Callinectes sapidus from Mediterranean Lagoons.

    Science.gov (United States)

    Mutlu, Cengiz; Türkmen, Mustafa; Türkmen, Aysun; Tepe, Yalçın

    2011-09-01

    This study was performed to investigate the metal concentrations in muscle and gill of blue crab, Callinectes sapidus from Dörtyol Lake, Akyatan Lagoon, Paradeniz Lagoon and Çamlık Lagoon from the northeastern coastal area of Mediterranean Sea. So, the levels of cadmium, chromium, copper, iron, manganese, nickel, aluminum and zinc in tissues of specimens from the lagoons were determined by inductively coupled plasma optical emission spectrometer. The metal concentrations found in muscle tissue varied for Cd: 0.03-0.08, Cr: 0.05-0.13, Cu: 5.38-11.7, Fe: 21.1-38.2, Mn: 0.15-2.98, Ni: 0.24-0.45, Zn: 13.9-20.1 and Al: 1.2-13.7 mg/kg wet weight. Iron showed the highest levels in both tissues, and generally followed by zinc except gills. On the other hand, cadmium showed the lowest levels from all sites. Followed cadmium; chromium had the second lowest levels. The differences between mean metal concentrations from different sampling sites were statistically significant. Regional changes in metal concentration were observed in the tissues of the crabs, but these variations may not influence consumption advisories.

  8. The male blue crab, Callinectes sapidus, uses both chromatic and achromatic cues during mate choice.

    Science.gov (United States)

    Baldwin, Jamie; Johnsen, Sönke

    2012-04-01

    In the blue crab, Callinectes sapidus, claw color varies by sex, sexual maturity and individual. Males rely in part on color cues to select appropriate mates, and these chromatic cues may be perceived through an opponent interaction between two photoreceptors with maximum wavelength sensitivities at 440 and 508 nm. The range of color discrimination of this dichromatic visual system may be limited, however, and it is unclear whether male blue crabs are capable of discriminating the natural variations in claw color that may be important in mate choice. By testing males' innate color preferences in binary choice tests between photographs of red-clawed females and six variations of orange-clawed females, we examined both the chromatic (opponent interaction) and achromatic (relative luminance) cues used in male mate choice. Males significantly preferred red-clawed females to orange-clawed females, except when the test colors were similar in both opponency and relative luminance. Our results are unusual in that they indicate that male mate choice in the blue crab is not guided solely by achromatic or chromatic mechanisms, suggesting that both color and intensity are used to evaluate female claw color.

  9. Evaluation of Blue Crab, Callinectes sapidus, Megalopal Settlement and Condition during the Deepwater Horizon Oil Spill.

    Science.gov (United States)

    Grey, Erin K; Chiasson, Susan C; Williams, Hannah G; Troeger, Victoria J; Taylor, Caz M

    2015-01-01

    The Blue Crab, Callinectes sapidus, is a commercially, culturally, and ecologically significant species in the Gulf of Mexico (GOM), whose offshore stages were likely impacted by the Deepwater Horizon oil spill (DWH). To test for DWH effects and to better understand the planktonic ecology of this species, we monitored Callinectes spp. megalopal settlement and condition at sites within and outside of the spill extent during and one year after the DWH. We tested for DWH effects by comparing 2010 settlement against baseline data available for two sites, and by testing for differences in settlement and condition inside and outside of the spill extent. We also developed time series models to better understand natural drivers of daily settlement variation (seasonal and lunar trends, hydrodynamics, wind) during 2010 and 2011. Overall, we found that neither megalopal settlement nor body weight were significantly reduced at oiled sites, but that high unexplained variation and low statistical power made detection of even large effects unlikely. Time series models revealed remarkably consistent and relatively strong seasonal and lunar trends within sites (explaining on average 28% and 9% of variation, respectively), while wind and hydrodynamic effects were weak (1-5% variation explained) and variable among sites. This study provides insights into DWH impacts as well as the natural drivers of Callinectes spp. megalopal settlement across the northern GOM.

  10. Photo-induced toxicity of Deepwater Horizon slick oil to blue crab (Callinectes sapidus) larvae.

    Science.gov (United States)

    Alloy, Matthew M; Boube, Idrissa; Griffitt, Robert J; Oris, James T; Roberts, Aaron P

    2015-09-01

    The 2010 Deepwater Horizon oil spill resulted in the accidental release of approximately 700 million L of crude oil into the Gulf of Mexico. Photo-induced toxicity after co-exposure to ultraviolet (UV) radiation is 1 mechanism by which polycyclic aromatic hydrocarbons (PAHs) from oil spills may exert toxicity. Blue crab are an important commercial and ecological resource in the Gulf of Mexico, and their largely transparent larvae may make them sensitive to PAH photo-induced toxicity. The goal of the present study was to examine the sensitivity of early lifestage blue crab (Callinectes sapidus) zoea to slick oil collected during the Deepwater Horizon spill. Blue crab zoea were exposed to 1 of several dilutions of water accommodated fractions from 1 of 2 sources of oil and gradations of natural sunlight in a factorial design. Two 7-h solar exposures were carried out with a recovery period (dark) in between. Survival was found to be UV- and PAH-dependent. Toxicity was observed within the range of surface PAH concentrations reported in the Gulf of Mexico during the Deepwater Horizon spill. These findings indicate that early lifestage blue crab are sensitive to photo-induced toxicity from Deepwater Horizon slick oil. © 2015 SETAC.

  11. Prevalence and distribution of three protozoan symbionts in blue crab (Callinectes sapidus) populations across Louisiana, USA.

    Science.gov (United States)

    Rogers, Holly A; Taylor, Sabrina S; Hawke, John P; Anderson Lively, Julie A

    2015-05-11

    Louisiana has one of the largest blue crab (Callinectes sapidus) fisheries in the USA, but little is known about blue crab diseases, parasites, and symbionts in this area. In 2013-2014, large juvenile and adult blue crabs were collected at 4 diverse sites to determine the prevalence of the protozoan symbionts associated with black gill disease (Lagenophrys callinectes), buckshot crabs (Urosporidium crescens), and bitter crab disease (Hematodinium perezi). A high aggregate prevalence of L. callinectes (93.2%) was identified across all seasons at all 4 collection sites regardless of salinity. A moderately low aggregate prevalence of U. crescens (22.4%) was identified across all seasons and sites. Prevalence of U. crescens depended on site salinity, with only 10% of infections detected at sites with callinectes and U. crescens are commensal parasites of blue crabs, infections can result in unmarketable and unappealing meat. In the Louisiana fishery, H. perezi has been blamed circumstantially for adult mortalities in the low salinity nearshore fishing grounds. Despite this, H. perezi was not detected in any of the large crabs sampled, even from the low salinity sites. The prevalence data reported here for these 3 protozoans are the first to include blue crabs sampled seasonally at multiple locations along the Louisiana coast over the period of a year.

  12. Environmental Stress and Pathogen Dynamics in the Blue Crab Callinectes sapidus

    Science.gov (United States)

    Sullivan, T. J.; Neigel, J.; Gelpi, C. G.

    2016-02-01

    The blue crab Callinectes sapidus is an ecologically and economically valuable species along the Gulf of Mexico and Atlantic coasts of North America. Throughout its range, the blue crab encounters a diverse array of parasitic and pathogenic microorganisms that have episodic and occasionally severe impacts on population numbers and viability. This makes understanding factors that influence pathogen dynamics, such as host stress, an important priority. To explore the role of environmental stress on the susceptibility of blue crabs to pathogens we screened individuals collected during the summers of 2014 and 2015 for a number of infectious agents. We sampled three life stages (megalopae, juvenile, and adult) from multiple marsh and offshore locations in Louisiana. Duration of stressful environmental conditions at each location was quantified from hourly recordings provided by the Louisiana Coastwide Reference Monitoring System. Pathogenic microorganisms were detected in crabs from multiple locations and multiple years. Some of the variability in prevalence of infection can be explained by exposure to stressful extremes of temperature and salinity during summer months.

  13. Evaluation of Blue Crab, Callinectes sapidus, Megalopal Settlement and Condition during the Deepwater Horizon Oil Spill.

    Directory of Open Access Journals (Sweden)

    Erin K Grey

    Full Text Available The Blue Crab, Callinectes sapidus, is a commercially, culturally, and ecologically significant species in the Gulf of Mexico (GOM, whose offshore stages were likely impacted by the Deepwater Horizon oil spill (DWH. To test for DWH effects and to better understand the planktonic ecology of this species, we monitored Callinectes spp. megalopal settlement and condition at sites within and outside of the spill extent during and one year after the DWH. We tested for DWH effects by comparing 2010 settlement against baseline data available for two sites, and by testing for differences in settlement and condition inside and outside of the spill extent. We also developed time series models to better understand natural drivers of daily settlement variation (seasonal and lunar trends, hydrodynamics, wind during 2010 and 2011. Overall, we found that neither megalopal settlement nor body weight were significantly reduced at oiled sites, but that high unexplained variation and low statistical power made detection of even large effects unlikely. Time series models revealed remarkably consistent and relatively strong seasonal and lunar trends within sites (explaining on average 28% and 9% of variation, respectively, while wind and hydrodynamic effects were weak (1-5% variation explained and variable among sites. This study provides insights into DWH impacts as well as the natural drivers of Callinectes spp. megalopal settlement across the northern GOM.

  14. Uptake and survival of enteric viruses in the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Hejkal, T W; Gerba, C P

    1981-01-01

    Uptake of poliovirus 1 by the blue crab, Callinectes sapidus, was measured to assess the likelihood of contamination by human enteric viruses. Virus was found in all parts of the crab within 2 h after the crab was placed in contaminated artificial seawater. The highest concentrations of virus were found in the hemolymph and digestive tract, but the meat also contained virus. The concentration of virus in the crabs was generally less than in the surrounding water. Changes in salinity did not substantially affect the rate of accumulation. An increase in temperature from 15 to 25 degrees C increased the rates of both uptake and removal. Poliovirus survived up to 6 days in crabs at a temperature of 15 degrees C and a salinity of 10 g/kg. When contaminated crabs were boiled, 99.9% of poliovirus 1, simian rotavirus SA11, and a natural isolate of echovirus 1 were inactivated within 8 min. These data demonstrate that viruses in crabs should not pose a serious health hazard if recommended cooking procedures are used. PMID:6261683

  15. High Connectivity among Blue Crab (Callinectes sapidus Populations in the Western South Atlantic.

    Directory of Open Access Journals (Sweden)

    Ana Luzia Figueiredo Lacerda

    Full Text Available Population connectivity in the blue crab Callinectes sapidus was evaluated along 740 km of the Western South Atlantic coast. Blue crabs are the most exploited portunid in Brazil. Despite their economic importance, few studies report their ecology or population structure. Here we sampled four estuarine areas in southern Brazil during winter 2013 and summer 2014 in order to evaluate diversity, gene flow and structure of these populations. Nine microsatellite markers were evaluated for 213 adult crabs, with identification of seven polymorphic loci and 183 alleles. Pairwise FST values indicated low population structure ranging from -0.00023 to 0.01755. A Mantel test revealed that the geographic distance does not influence genetic (r = -0.48, and structure/migration rates confirmed this, showing that even the populations located at the opposite extremities of our covered region presented low FST and exchanged migrants. These findings show that there is a significant amount of gene flow between blue crab populations in South Brazil, likely influenced by local current dynamics that allow the transport of a high number of larvae between estuaries. Considering the elevated gene flow, the populations can be considered a single genetic stock. However, further information on population size and dynamics, as well as fishery demands and impacts at different regions, are necessary for harvest management purposes.

  16. A Lethal Virus of the Blue Crab Callinectes sapidus May Be Present Throughout its Trans-hemispheric Range

    Science.gov (United States)

    Schott, E. J.; Flowers, E. M.; Vinagre, A.; Brown, S.; Almeida, A.

    2016-02-01

    Blue crabs, Callinectes sapidus, are key epibenthic predators and support valuable fisheries throughout North and South America. On both continents, they are also used to produce soft shell crabs, a value added product. On the Atlantic coast of the USA, mortality of blue crabs in soft shell production is often 25% or greater. A majority of those crabs have been found to be infected with a reovirus lethal to blue crabs. The CsRV1 virus infects multiple ectodermal and mesodermal tissues and was first described as RLV (reo-like virus) in the late 1970s. However its potential for affecting large numbers of crabs was not fully recognized until 2010 when molecular methods were developed to study its prevalence in aquaculture and the wild. Using a sensitive Rt-qPCR assay for the virus, a multi-year survey of crabs from the mid-Atlantic USA coast and Chesapeake Bay revealed that approximately 20% of adult and juvenile crabs were positive for CsRV1 genomic RNA. If blue crabs in the wild die from CsRV1 infections as rapidly as captive infected crabs, it suggests that significant numbers of wild blue crabs die from CsRV1 infections. Crab fishery managers in the Chesapeake Bay recently recognized that there is a need to better understand the role of diseases in natural mortality. The CsRV1 virus is found in C. sapidus throughout the USA range, and has been discovered in diseased Brazilian C. sapidus from Rio Grande do Sul. Genetic analysis of the virus shows that strains of CsRV1 found in Brazil are distinct from the strains identified in the USA. This pan-hemispheric range suggests that the virus has long been a component of blue crab ecology. Much more study is needed to understand the history and impacts of CsRV1 throughout the USA, Brazil, and the vast coastline in between.

  17. An insulin-like growth factor found in hepatopancreas implicates carbohydrate metabolism of the blue crab Callinectes sapidus.

    Science.gov (United States)

    Chung, J Sook

    2014-04-01

    Hyperglycemia that is caused by the release of crustacean hyperglycemic hormone (CHH) from the sinus gland to hemolymph is one of the hallmark physiological phenomena, occurring in decapod crustaceans experiencing stressful conditions. However, the mechanism(s) by which such elevated glucose levels return to resting levels is still unknown. Interestingly, noted is a difference in the clearance rate of hemolymph glucose between adult females and adult males of the blue crab, Callinectes sapidus: the former with more rapid clearance than the latter. The presence of an endogenous-insulin-like molecule is suggested in C. sapidus because an injection of bovine insulin, significantly reduces the levels of hemolymph glucose that were previously elevated by emersion stress or the glucose injection. Using 5' and 3' RACE, the full-length cDNA of an insulin-like molecule is isolated from the hepatopancreas of an adult female C. sapidus and shows the same putative sequence of an insulin-like androgenic gland factor (IAG) but differs in 5' and 3' UTR sequences. A knock-down study using five injections of double-stranded RNA of CasIAG-hep (dsRNA-CasIAG-hep, 10μg/injection) over a 10-day period reduces CasIAG-hep expression by ∼50%. The levels of hemolymph glucose are also kept higher in dsRNA-CasIAG-hep injected group than those treated with dsRNA-green fluorescent protein (dsRNA-IAG-hep) or saline. Most importantly, the hepatopancreas of dsRNA-CasIAG-hep injected animals contains amounts of carbohydrate (glucose, trehalose, and glycogen) significantly lower than those of control groups, indicating that the function of CasIAG-hep in carbohydrate metabolism in crustaceans is similar to carbohydrate metabolism in vertebrates. Copyright © 2014 Elsevier Inc. All rights reserved.

  18. Cloning of aquaporin-1 of the blue crab, Callinectes sapidus: its expression during the larval development in hyposalinity.

    Science.gov (United States)

    Chung, J Sook; Maurer, Leah; Bratcher, Meagan; Pitula, Joseph S; Ogburn, Matthew B

    2012-09-03

    Ontogenetic variation in salinity adaptation has been noted for the blue crab, Callinectes sapidus, which uses the export strategy for larval development: females migrate from the estuaries to the coast to spawn, larvae develop in the ocean, and postlarvae (megalopae) colonize estuarine areas. We hypothesized that C. sapidus larvae may be stenohaline and have limited osmoregulatory capacity which compromises their ability to survive in lower salinity waters. We tested this hypothesis using hatchery-raised larvae that were traceable to specific life stages. In addition, we aimed to understand the possible involvement of AQP-1 in salinity adaptation during larval development and during exposure to hyposalinity. A full-length cDNA sequence of aquaporin (GenBank JQ970426) was isolated from the hypodermis of the blue crab, C. sapidus, using PCR with degenerate primers and 5' and 3' RACE. The open reading frame of CasAQP-1 consists of 238 amino acids containing six helical structures and two NPA motifs for the water pore. The expression pattern of CasAQP-1 was ubiquitous in cDNAs from all tissues examined, although higher in the hepatopancreas, thoracic ganglia, abdominal muscle, and hypodermis and lower in the antennal gland, heart, hemocytes, ovary, eyestalk, brain, hindgut, Y-organs, and gill. Callinectes larvae differed in their capacity to molt in hyposalinity, as those at earlier stages from Zoea (Z) 1 to Z4 had lower molting rates than those from Z5 onwards, as compared to controls kept in 30 ppt water. No difference was found in the survival of larvae held at 15 and 30 ppt. CasAQP-1 expression differed with ontogeny during larval development, with significantly higher expression at Z1-2, compared to other larval stages. The exposure to 15 ppt affected larval-stage dependent CasAQP-1 expression which was significantly higher in Z2- 6 stages than the other larval stages. We report the ontogenetic variation in CasAQP-1 expression during the larval development

  19. Note on a Collection of Decapoda Branchyura from the Coasts of Mexico, includung the Description of a new Genus and Species

    NARCIS (Netherlands)

    Buitendijk, A.M.

    1950-01-01

    The material of Brachyura on which this paper is based was collected during some years on the Mexican coasts and I am greatly indebted to Dr. F. Bonet of the Escuela Nacional de Ciencias Biologicas in Mexico, D. F., for the opportunity he offered me to study this interesting material and for his

  20. Un caso de hipertrofia bilateral de quelas en el cangrejo violinista Uca cumulanta (Decapoda: Ocypodidae

    Directory of Open Access Journals (Sweden)

    Carlos Lira

    2006-12-01

    Full Text Available Un macho de Uca cumulanta con hipertrofia bilateral de quelas fue capturado durante en la Laguna de La Restinga, Isla de Margarita, Venezuela. Ambas quelas eran subiguales en tamaño y se asemejaban al quelípedo mayor de los machos normales.A case of bilateral cheliped hypertrophya crab Uca cumulanta (Decapoda: Ocypodidae. An adult male of Uca cumulanta with bilateral cheliped hypertrophy was found during a collection of crabs at La Restinga Lagoon, Margarita Island, Venezuela. Both chelipeds were sub equal in size, regarding the major cheliped of a normal male. Rev. Biol. Trop. 54 (Suppl. 3: 117-119. Epub 2007 Jan. 15.

  1. Risk of Handling as a Route of Exposure to Infectious Waterborne Cryptosporidium parvum Oocysts via Atlantic Blue Crabs (Callinectes sapidus)▿

    Science.gov (United States)

    Graczyk, Thaddeus K.; McOliver, Cynthia; Silbergeld, Ellen K.; Tamang, Leena; Roberts, Jennifer D.

    2007-01-01

    Commercial Atlantic blue crabs (Callinectes sapidus) were exposed to 2.0 × 104 infectious waterborne oocysts of Cryptosporidium parvum. The study demonstrated that blue crabs can transfer C. parvum oocysts to persons involved in handling or preparing crabs and that they may contaminate other surfaces or products during storage. PMID:17449680

  2. A trehalose 6-phosphate synthase gene of the hemocytes of the blue crab, Callinectes sapidus: cloning, the expression, its enzyme activity and relationship to hemolymph trehalose levels.

    Science.gov (United States)

    Chung, J Sook

    2008-12-12

    Trehalose in ectoderms functions in energy metabolism and protection in extreme environmental conditions. We structurally characterized trehalose 6-phosphate synthase (TPS) from hemocytes of the blue crab, Callinectes sapidus. C. sapidus Hemo TPS (CasHemoTPS), like insect TPS, encodes both TPS and trehalose phosphate phosphatase domains. Trehalose seems to be a major sugar, as it shows higher levels than does glucose in hemocytes and hemolymph. Increases in HemoTPS expression, TPS enzyme activity in hemocytes, and hemolymph trehalose levels were determined 24 h after lipopolysaccharide challenge, suggesting that both TPS and TPP domains of CasHemoTPS are active and functional. The TPS gene has a wide tissue distribution in C. sapidus, suggesting multiple biosynthetic sites. A correlation between TPS activity in hemocytes and hemolymph trehalose levels was found during the molt cycle. The current study provides the first evidence of presence of trehalose in hemocytes and TPS in tissues of C. sapidus and implicates its functional role in energy metabolism and physiological adaptation.

  3. The occurrence of Callinectes arcuatus ordway, 1863 (Crustacea, Decapoda, Portunidae in Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Edilson Pires de Gouvêa

    1987-01-01

    Full Text Available During the study of the Carcinofauna of the Bahian Coast, some Brachyura Portunidae were found. Some of these animals were Callinectes arcuatus Ordway, 1863 which has its distribution restricted to the Pacific Ocean, from California to Peru and the Galapagos Islands. This is the first occurrence of this species reported from the Atlantic Ocean and the Brazilian Coast (Bahia, 38º50'Wand 12º50'S.Durante o estudo da carcinofauna do litoral baiano, muitos Braquiuros Portunidae foram encontrados. Alguns destes animais foram Callinectes arcuatus Ordway, 1863 que possuia sua distribuição restrita da Califórnia ao Peru e Ilhas Galápagos, no Oceano Pacífico. Esta é a primeira ocorrência desta espécie no Oceano Atlântico e na Costa Brasileira. (Bahia, 12º50'S e 38º50'W.

  4. Spontaneous alternation and locomotor activity in three species of marine crabs: green crab (Carcinus maenas), blue crab (Callinectes sapidus), and fiddler crab (Uca pugnax).

    Science.gov (United States)

    Balcı, Fuat; Ramey-Balcı, Patricia A; Ruamps, Perrine

    2014-02-01

    Spontaneous alternation refers to the tendency of organisms to explore places that they have least recently visited. Our previous work showed that alternation performance of Carcinus maenas (invasive European green crab) was significantly higher than Callinectes sapidus (native blue crab), and chance level performance (Ramey, P. A., Teichman, E., Oleksiak, J., & Balcı, F. [2009]. Spontaneous alternation in marine crabs: Invasive versus native species. Behavioural Processes, 82, 51-55.). In the current study, we first tested the robustness of these findings in the absence of visual cues, longer test durations, and wider maze dimensions. These manipulations enabled us to determine whether these two crab species relied on the visual cues provided during the spontaneous alternation task in our prior work, and allowed for better characterization of their exploratory activity in the maze. Our original findings were reproduced in the present study under these new task conditions, suggesting no role for visual cues during alternation, and emphasizing the robustness and generalizability of the corresponding interspecies differences in alternation performance. We also tested whether the lower alternation performance of C. sapidus also applied to another native crab species, Uca pugnax (fiddler crab). Spontaneous alternation performance of U. pugnax was significantly lower than C. maenas but indistinguishable from C. sapidus. Finally, we examined whether the potentially higher inherent risk-sensitivity of C. sapidus could have contributed to their lower alternation performance by testing C. maenas in the presence of a larger natural predator (stressor). Higher risk sensitivity presumably induced by the stressor led to locomotor activity patterns that better resembled those of C. sapidus, however the resultant reduction in alternation performance was not statistically significant. PsycINFO Database Record (c) 2014 APA, all rights reserved.

  5. Hemolymph ecdysteroids during the last three molt cycles of the blue crab, Callinectes sapidus: quantitative and qualitative analyses and regulation.

    Science.gov (United States)

    Chung, J Sook

    2010-01-01

    The profiles of circulating ecdysteroids during the three molt cycles prior to adulthood were monitored from the juvenile blue crab, Callinectes sapidus. Ecdysteroid patterns are remarkably similar in terms of peak concentrations ranging between 210-330 ng/ml hemolymph. Analysis of hemolymph at late premolt stage revealed six different types of ecdysteroids with ponasterone A (PoA) and 20-OH ecdysone (20-OH E) as the major forms. This ecdysteroid profile was consistent in all three molt cycles. Bilateral eyestalk ablation (EA) is a procedure that removes inhibitory neurohormones including crustacean hyperglycemic hormone (CHH) and molt-inhibiting hormone (MIH) and often results in precocious molting in crustaceans. However, the inhibitory roles of these neuropeptides in vivo have not yet been tested in C. sapidus. We determined the regulatory roles of CHH and MIH in the circulating ecdysteroid from ablated animals through daily injection. A daily administration of purified native CHH and MIH at physiological concentration maintained intermolt levels of ecdysteroids in the EA animals. This suggests that Y organs (YO) require a brief exposure to CHH and MIH in order to maintain the low level of ecdysteroids. Compared to intact animals, the EA crabs did not exhibit the level of peak ecdysteroids, and the major ecdysteroid turned out to be 20-OH E, not PoA. These results further underscore the important actions of MIH and CHH in ecdysteroidogenesis, as they not only inhibit, but also control the composition of output of the YO activity. (c) 2009 Wiley Periodicals, Inc.

  6. Extracellular digestion during hyposaline exposure in the Dungeness crab, Cancer magister, and the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Curtis, Daniel L; van Breukelen, Frank; McGaw, Iain J

    2013-12-01

    Extracellular digestive processes were examined in the Dungeness crab, Cancer magister and the blue crab, Callinectes sapidus, during hyposaline exposure. Both species are found in estuaries as adults, but vary in their ability to balance the cardiovascular and respiratory demands of concurrent osmoregulation and digestion. The weak osmoregulator, C. magister, is unable to balance the demands of osmoregulation and digestion. Concordant with observed decreases in oxygen consumption and mechanical digestion, proteolytic digestion within the foregut and hepatopancreas was delayed, resulting in a relative reduction of circulating amino acids post-feeding in low salinity. In contrast, the efficient osmoregulator, C. sapidus, balances the demands of osmoregulation and digestion, and mechanical digestion continues unabated in low salinity. Protease activity in the gut fluid and hepatopancreas showed either no change or a reduction over time. The transport of amino acids into the cells post-feeding is opposed by an efflux of amino acids at the cellular level, and resulted in a build up of amino acids in the hemolymph. Despite differences in the extracellular responses to low salinity exposure following feeding, both species were able to maintain high digestive efficiencies. © 2013.

  7. Temperature correlates with annual changes in Hematodinium perezi prevalence in blue crab Callinectes sapidus in Florida, USA.

    Science.gov (United States)

    Gandy, Ryan; Schott, Eric J; Crowley, Claire; Leone, Erin H

    2015-04-08

    Blue crabs Callinectes sapidus were monitored biannually throughout Florida, USA, for 2 yr using a highly sensitive, quantitative polymerase chain reaction (qPCR) to determine the spatial and temporal changes in prevalence and intensity of Hematodinium perezi infections during drought years. Despite persistent drought conditions, H. perezi infections were not universally found. Overall prevalence was 25.3% (95% CI: 22.8-28.1%) in 1066 crabs sampled from 6 locations (Jacksonville, Ormond Beach, Everglades City, Tampa Bay, Steinhatchee, and Panama City) from 2011 to 2012. Presence of H. perezi was consistently highest in winter season samples, ranging from 4.2-51.1% (3 locations) in 2011, to 32-83% (5 locations) in 2012. The highest prevalence and intensities were observed in the winter samples from Everglades City. Previous studies have found that the prevalence of H. perezi in C. sapidus in temperate regions of the US East Coast shows seasonal peaks in early winter in Maryland and South Carolina and in fall and spring in Georgia. The seasonality of infections in the subtropical waters of Florida reinforces the concept that temperature is a strong factor that may override other drivers, such as drought. Seasonal H. perezi infections in Florida appear to be triggered by the parasite responding to an optimal temperature during the annual rise from the low temperature of winter when salinity is elevated. However, salinity alone is not sufficient to trigger an increase in prevalence of H. perezi in Florida.

  8. Effects of hexavalent chromium on development of crabs, Rhithropanopeus harrisii and Callinectes sapidus

    Energy Technology Data Exchange (ETDEWEB)

    Bookhout, C.G.; Monroe, R.J.; Forward, R.B. Jr.; Costlow, J.D. Jr.

    1984-01-01

    Survival of Rhithropanopeus harrisii larvae from hatching to first crab stage occurred in Na/sub 2/CrO/sub 4/ concentrations from 1.1 to 29.1 ppm. Estimated LC50 for complete zoeal development was 17.8 ppm Na/sub 2/CrO/sub 4/ and it was 13.7 ppm for development to first crab stage. A concentration of 1.1 ppm Na/sub 2/CrO/sub 4/ was nontoxic, while Na/sub 2/CrO/sub 4/ concentrations of 7.2 and 14.5 ppm were sublethal and concentrations of 29.1 to 58.1 ppm were acutely toxic. Low concentrations of Na/sub 2/CrO/sub 4/ concentrations of Na/sub 2/CrO/sub 4/ caused an increase in swimming speed and high concentrations caused a decline. Survival of Callinectes sapidus larvae occurred in Na/sub 2/CrO/sub 4/ concentrations from 1.1 to 4.7 ppm. The LC50 for complete zoeal development was estimated to be 2.9 ppm Na/sub 2/CrO/sub 4/ and the LC50 for development to first crab stage was estimated to be 1.0 ppm Na/sub 2/CrO/sub 4/. The total Cr in sodium chromate is 32% by weight, hence, the total Cr concentrations tested were 32% of the Cr salts given above. Statistical analyses of the data on survival, duration and mortality of larvae are presented.

  9. Locomotory fatigue during moderate and severe hypoxia and hypercapnia in the Atlantic blue crab, Callinectes sapidus.

    Science.gov (United States)

    Stover, Kristin K; Burnett, Karen G; McElroy, Eric J; Burnett, Louis E

    2013-04-01

    The Atlantic blue crab, Callinectes sapidus (Rathbun), is a highly mobile crustacean that must locomote to find food, evade predators, find mates, and avoid adverse conditions such as hypoxia. In this study we tested the effects of two levels of hypoxia (10.4 kPa, 50% air saturation = moderate hypoxia; 4 kPa, 20% air saturation = severe hypoxia) and hypercapnic hypoxia (50% air saturation O(2) with Pco(2) = 2 kPa) on fatigue during sustained continuous exercise. Fatigue was induced by an exercise trial that entailed continuous sideways hexapedal walking on an underwater treadmill. Fatigue was quantified using two methods: (1) a pull force test that measures the holding strength of the legs, and (2) the number of fatigue-resisting behaviors (180° turns and stopping). Fatigue was defined as a pull force of 67% or less of the initial pre-exercise pull force and was reached after 6.12 h of walking for crabs in well-aerated normoxic seawater, 4 h in 50% air saturation, 2.07 h in 20% air saturation, and 4.58 h in 50% air saturation and hypercapnia. The number of fatigue-resisting behaviors increased with walking time in all treatments. Performance decreased in hypoxia, with fatigue being reached more quickly as the level of hypoxia intensified. Hypercapnia in moderate hypoxia did not have a deleterious influence on behavior and lengthened slightly the time it took crabs to fatigue. In addition, severe hypoxia exacerbated changes in gait kinematics as crabs became fatigued, by significantly increasing stride length and decreasing stride frequency.

  10. Toxicity of the organophosphate insecticide fenitrothion and its metabolism by blue crabs, Callinectes sapidus

    Energy Technology Data Exchange (ETDEWEB)

    Johnston, J.J.

    1986-01-01

    The LC/sub 50/ for Callinectes sapidus exposed to fenitrothion for 96 hours at 22/sup 0/C and a salinity of 34 ppt (parts per thousand) was estimated to be 8.6 ..mu..g/liter. Acute toxicity was shown to decrease with decreasing salinity and decreasing temperature. Exposure to a simulated tidal cycle increased the acute toxicity of fenitrothion to Callinectes. The autotomization response in Callinectes was shown to be affected at subacute exposure levels as low as 0.1 ..mu..g/liter. In vitro studies showed that the rates of formation of 3-methyl-4-nitrophenol and desmethyl fenitrothion were greater and the rate of formation of fenitrooxon was less in subcellular fractions prepared from the hepatopancreas of crabs which had been acclimated to lower salinity seawater. All three of these metabolites were formed at faster rates in subcellular fractions prepared from crabs which had been acclimated to and incubated at 22/sup 0/C than at 17/sup 0/C. Tissue distribution of aniline hydroxylase and glutathione-S-transferase activity was also determined. The uptake of /sup 14/C-fenitrothion at a level in a seawater of 5.2 ..mu..g/liter was greater at 22/sup 0/C than at 17/sup 0/C and from 34 ppt than from 17 ppt seawater. Tissue distribution of radioactivity was determined as well as the nature of radiolabelled metabolites in the hepatopancreas and in seawater. There was no significant difference in the overall level of metabolites detected in the 22/sup 0/C than in the 17/sup 0/C seawater. The rate of uptake of fenitrothion by Callinectes also affects the toxicity as the uptake of fenitrothion was more rapid at the higher salinity as well as at the higher temperature.

  11. Analysis of metals in blue crabs, Callinectes sapidus, from two Connecticut estuaries

    Energy Technology Data Exchange (ETDEWEB)

    Jop, K.M. [Sciences Applications International Corp., Narragansett, RI (United States); Biever, R.C.; Hoberg, J.R.; Shepherd, S.P. [Springborn Labs., Inc., Wareham, MA (United States)

    1997-02-01

    Decisions involving the use and allocation of resources in the nation`s coastal and estuarine regions require reliable and continuous information about the status and trends of environmental quality in those areas. In recent years much attention has been focused on assessing environmental risks resulting from the manufacture, distribution, use and disposal of chemicals. Legislation and public concern have produced numerous regulations concerning the introduction of chemicals into surface waters which increase the potential for human exposure. There is considerable concern about the human health aspects of metal cycling especially in coastal and inland waters that are in proximity to large populations or industrial centers. Many of these compounds tend to remain in the ecosystem and eventually move from lower to higher trophic levels within the human food chain. Blue crabs, Callinectes sapidus Rathbun, are important members of the estuarine food chain due to high abundance and their multiple role as scavengers, predators and prey. Because of their omnivorous feeding characteristics and association with sediments, blue crabs are exposed and may potentially accumulate significant amount of pollutants above background seawater concentrations. This species represents one of the most valuable fishery resources in the southern states. According to Marcus and Mathews blue crabs ranked fifth in 1984 and fourth in 1985 behind shrimp, swordfish, oysters and hard clams in economic value, but first in total weight for both years in South Carolina. Although in Connecticut blue crabs are harvested mostly for personal consumption, their commercial value in other states makes the results from any environmental survey of great interest from the consideration of risk to human health. This study evaluated the level of contaminants in Blue crabs in the summer months by examining the concentration of selected metals in samples of muscle and hepatopancreas tissue. 12 refs., 3 tabs.

  12. Molecular detection of the parasitic dinoflagellate Hematodinium perezi from blue crabs Callinectes sapidus in Louisiana, USA.

    Science.gov (United States)

    Sullivan, Timothy J; Gelpi, Carey G; Neigel, Joseph E

    2016-06-15

    The dinoflagellate Hematodinium perezi is a prolific pathogen of the blue crab Callinectes sapidus along the Atlantic and Gulf of Mexico coasts of North America. High prevalence, sometimes approaching 100%, and outbreaks with high mortality are associated with higher salinities. H. perezi has not been reported previously in blue crabs from Louisiana, USA, where salinities in coastal habitats are generally below the parasite's favorable range. However, the possibility that H. perezi infects blue crabs in higher salinity habitats offshore has not been investigated. A PCR-based test for H. perezi was used to screen blue crabs collected from both high and low salinity areas. These included juvenile and adult crabs from inshore marshes where salinities are relatively low and from higher salinity offshore shoals that are spawning sites for females. H. perezi was detected in blue crabs from offshore shoals (prevalence=5.6%) but not in juvenile or adult crabs from inshore habitats. Megalopae (post-larvae) were also collected from inshore locations. Although megalopae settle inshore where salinities are relatively low, the megalopal stage is preceded by a planktonic phase in higher salinity offshore waters. We detected H. perezi in 11.2% of settling megalopae tested. Although the prevalence of H. perezi was relatively low within our samples, if spawning females and megalopae are especially vulnerable, the impact on the population could be compounded. This is the first report of H. perezi from blue crabs in Louisiana and demonstrates the importance of examining all life stages in determining the prevalence of a harmful parasite.

  13. Lack of transmission of Hematodinium sp. in the blue crab Callinectes sapidus through cannibalism.

    Science.gov (United States)

    Li, Caiwen; Wheeler, Kersten N; Shields, Jeffrey D

    2011-10-06

    Hematodinium spp. are parasitic dinoflagellates of marine crustaceans. Outbreaks of Hematodinium sp. have impacted commercial landings of the blue crab Callinectes sapidus in the coastal bays of Virginia and Maryland (USA), with seasonal peaks in prevalence reaching 85%. The life cycle and transmission routes of the parasite in blue crabs are poorly understood. Cannibalism and waterborne transmission may be routes of transmission, although little conclusive evidence has been reported for these modes. We examined cannibalism as a route by a series of experiments wherein we repeatedly fed adult and juvenile crabs the tissues of crabs infected with Hematodinium. In each experiment, feeding was done 3 times over the course of 1 wk. Only 2 of 120 crabs were infected within 7 to 9 d after feeding, and these 2 were likely infected prior to the experimental exposures. Crabs inoculated with hemolymph from infected donors served as positive controls. They developed infections over 11 to 21 d, indicating that the Hematodinium sp. used in the cannibalism trials was infectious at the time of inoculation. Because amphipods also harbor Hematodinium-like infections, we fed tissues of infected crabs to the estuarine amphipod Leptocheirus plumulosus. Hematodinium DNA was detected in amphipods shortly after feeding, but not in animals held for longer periods, nor was it observed in histological preparations. Amphipods did not obtain infections by scavenging infected crab tissues. Our results show that Hematodinium sp. is not effectively transmitted through ingestion of diseased tissues, indicating that cannibalism may not be a major route of transmission for Hematodinium sp. in blue crabs.

  14. Functional characterization of neuroendocrine regulation of branchial carbonic anhydrase induction in the euryhaline crab Callinectes sapidus.

    Science.gov (United States)

    Mitchell, Reed T; Henry, Raymond P

    2014-12-01

    Carbonic anhydrase (CA) plays an essential role as a provider of counterions for Na(+)/H(+) and Cl(-)/HCO3 (-) exchange in branchial ionic uptake processes in euryhaline crustaceans. CA activity and gene expression are low in crabs acclimated to full-strength seawater, with transfer to low salinity resulting in large-scale inductions of mRNA and subsequent enzyme activity in the posterior ion-regulating gills (e.g., G7). In the green crab Carcinus maenas, CA has been shown to be under inhibitory neuroendocrine control by a putative hormone in the x-organ-sinus gland complex (XOSG), located in the eyestalk. This study characterizes the neuroendocrine regulation of CA induction in the blue crab Callinectes sapidus, a commonly used experimental organism for crustacean osmoregulation. In crabs acclimated to full-strength seawater, eyestalk ligation (ESL) triggered a 1.8- and 100-fold increase in CA activity and mRNA, respectively. Re-injection with eyestalk homogenates abolished increases in CA activity and fractionally reduced CA gene expression. ESL also enhanced CA induction by 33% after 96 h in crabs transferred to 15 ppt salinity. Injection of eyestalk homogenates into intact crabs transferred from 35 to 15 ppt diminished by 43% the CA induction stimulated by low salinity. These results point to the presence of a repressor hormone in the eyestalk. Separate injections of medullary tissue (MT) and sinus gland (SG), two components of the eyestalk, reduced salinity-stimulated CA activity by 22% and 49%, suggesting that the putative repressor is localized to the SG. Crabs injected with SG extract harvested from crabs acclimated to 5 ppt showed no decrease in CA activity, demonstrating that the hormone is down-regulated at low salinity. Our results show the presence in the XOSG of an inhibitory compound that regulates salinity-stimulated CA induction. © 2014 Marine Biological Laboratory.

  15. Isotopic determination of the trophic ecology of a ubiquitous key species - The crab Liocarcinus depurator (Brachyura: Portunidae)

    Science.gov (United States)

    Careddu, Giulio; Calizza, Edoardo; Costantini, Maria Letizia; Rossi, Loreto

    2017-05-01

    Knowledge of the trophic ecology of predators is key to understanding how they affect food web structure and ecosystem functioning. The harbour crab Liocarcinus depurator (L.) (Brachyura: Portunidae) is one of the most abundant decapod species in soft-bottom areas of the Mediterranean Sea and northeast Atlantic Ocean. It is both a common prey and predator of commercial and non-commercial marine species and its predation pressure appears to have little effect on the subtidal community assemblage. However, there are few studies of its diet and little is known about its role in mediating energy flows in marine ecosystems. In this study, carbon (δ13C) and nitrogen (δ15N) stable isotope analysis (SIA) and Bayesian analytical tools were used to characterise the trophic niche of L. depurator and to quantify the most important prey supporting this species under various environmental conditions. Specimens of L. depurator, their potential prey and basal resources were collected from two different subtidal areas of the Gulf of Gaeta, one affected by human activities (north side) and the other seasonally influenced by freshwater inputs originating from the River Garigliano (south side). While there were differences between the two sampling areas in terms of the abundance and δ15N and δ13C values of the macrobenthic prey community, no differences in the δ15N values and trophic position of L. depurator were observed. Specifically, Bayesian mixing models showed Polychaeta Errantia as the main source of crab diets in both areas. The observed differences in the δ13C values and the analysis of trophic pathways also indicate that the terrestrial organic matter originating from the discharge of the River Garigliano was integrated along the food web up to L. depurator. Although this species is usually considered an opportunistic feeder, it appears to be highly selective and its trophic habits did not influence food web topology, which in contrast was found to be strongly

  16. A new species of Foza Reed & Cumberlidge, 2006 from northern Madagascar (Decapoda, Brachyura, Potamoidea, Potamonautidae, with a redescription of F. goudoti (H. Milne Edwards, 1853 comb. n., and comments on Skelosophusa prolixa Ng & Takeda, 1994

    Directory of Open Access Journals (Sweden)

    Neil Cumberlidge

    2009-08-01

    Full Text Available Foza ambohitra sp. n. is described from Ambohitra, Antsiranana Province, northern Madagascar at 421 m elevation. This species is distinguished by characters of the carapace, the male anterior thoracic sternum, and the form of the male major cheliped and first gonopod. Thelphusa goudoti H. Milne Edwards, 1853, is transferred to Foza Reed & Cumberlidge, 2006, and redescribed, and a key to the three species of this genus is provided. Comments on the rare cave crab Skelosophusa prolixa Ng & Takeda, 1994, from Antsiranana Province are also included based on newly obtained material.

  17. Correct date and authorship of taxa of Middle American freshwater crabs described by Rodríguez & Smalley (1972) (not 1969) and included in Smalley (1970) (Crustacea: Decapoda: Brachyura: Pseudothelphusidae).

    Science.gov (United States)

    Guinot, Danièle; Hendrickx, Michel E

    2014-06-24

    One subgenus and one genus of Pseudothelphusidae described by Gilberto Rodríguez and Alfred E. Smalley from Mexico have been erroneously referred to for over 40 years as variously described in 1968 and 1969. The review of the original publication indicates that these taxa were published in a journal dated 1969 that became available for distribution only in 1972. Smalley (1970), who believed that the original manuscript had been previously published, referred to some of these new taxa (i.e., Epithelphusa, E. mixtepensis, Tehuana and T. veracruzana) and provided sufficient information to make these names available in 1970, thus becoming the correct authorship for these four taxa. Therefore they must be referred to as "Rodríguez & Smalley in Smalley 1970". A list of all affected taxa with the correct publication date and authorship is given. A list of publications in which the taxa authored by Rodríguez and Smalley were erroneously referred to as published in 1969 is also provided.

  18. Size structure and sex composition of Eurypodius latreillii Guérin, 1828 (Brachyura and Pagurus comptus White, 1847 (Anomura in southern Chile

    Directory of Open Access Journals (Sweden)

    R. Soto

    1999-12-01

    Full Text Available The decapod crustaceans Eurypodius latreillii (Brachyura and Pagurus comptus (Paguridae were the most frequently occurring species in Agassiz trawl (AGT samples obtained during the Chilean - German - Italian Victor Hensen Expedition in the Straits of Magellan and south of the Beagle Channel. Sex composition and length structure aspects were studied using the material from 15 stations in the two areas, which comprised 773 specimens of E. latreillii and 647 P. comptus. The size frequency of both species was characterized by the presence of small individuals in shallow waters. The sex composition varied with depth, indicating a dominance of E. latreillii females in deep waters, whereas no such pattern was observed in P. comptus. These differences may be caused by epizoites of E. latreillii and the availability of vacant gastropod shells for P. comptus, in addition to geographical aspects and life-cycle patterns of these species

  19. First record of Puerulus mesodontus Chan, Ma & Chu, 2013 (Crustacea, Decapoda, Achelata, Palinuridae) from south of Java, Indonesia.

    Science.gov (United States)

    Wardiatno, Yusli; Hakim, Agus Alim; Mashar, Ali; Butet, Nurlisa Alias; Adrianto, Luky; Farajallah, Achmad

    2016-01-01

    Three specimens of Puerulus mesodontus Chan, Ma & Chu, 2013 (Crustacea, Decapoda, Achelata, Palinuridae) were collected from Palabuhanratu Bay, southern Java, Indonesia. There is no previous record on the presence of the species in Indonesia. This finding represents the first record of this species in Java, Indonesia, and confirms that the species is present in the Indian Ocean. The morphological characters of the species are described. This paper contains a new distribution record of a lobster species from Indonesian waters.

  20. Efficacy of alfaxalone for intravascular anesthesia and euthanasia in blue crabs (Callinectes sapidus).

    Science.gov (United States)

    Minter, Larry J; Harms, Craig A; Archibald, Kate E; Broadhurst, Heather; Bailey, Kate M; Christiansen, Emily F; Lewbart, Gregory A; Posner, Lysa P

    2013-09-01

    The objective of this study was to characterize the behavioral effects and changes in heart rate of four doses of alfaxalone delivered by intravascular injection to blue crabs (Callinectes sapidus). Thirty (male, n = 27; female, n = 3) blue crabs were randomly assigned to one of four treatment groups of alfaxalone: eight animals were assigned to each of the 5-, 10-, and 15-mg/kg treatment groups, and the remaining six animals were assigned to the 100-mg/kg group. Times for anesthetic induction and recovery periods were recorded. Righting reflex, defensive posturing, and heart rate were evaluated before, during, and after the anesthetic trial. Anesthesia was induced in all 14 animals consolidated into the high-dosage group (15 mg/kg [n = 8] and 100 mg/kg [n = 6]), which was significantly greater than 8 of 16 animals in the low-dosage group (5 mg/kg [n = 2] and 10 mg/kg [n = 6]). Median anesthesia induction time for all crabs was 0.4 min, with no significant difference in induction time between groups observed. Median recovery time was 9.4 min (n = 2), 6.1 min (n = 5), 11.3 min (n = 8), and 66.1 min (n = 5) for the 5-, 10-, 15-, and 100-mg/kg groups, respectively. Recovery times were significantly longer for crabs exposed to an induction dose of 100 mg/kg compared with the 10- and 15-mg/kg induction doses. A significant decrease in the median heart rate was observed between the baseline value and that observed at both induction and 5 min postinjection in the 100-mg/kg dose trial. Two mortalities were observed during the anesthesia trials (n = 1, 10 mg/kg; n = 1, 100 mg/kg), both associated with the autotomization of limbs. In summary, the intravascular administration of alfaxalone at 15 mg/kg provided rapid and reliable sedation, whereas alfaxalone administered at 100 mg/kg produced rapid and long lasting anesthesia.

  1. CALCIUM PHOSPHATE GRANULES IN THE HEPATOPANCREAS OF THE BLUE CRAB CALLINECTES SAPIDUS

    Science.gov (United States)

    Becker, Gerald L.; Chen, Chung-Ho; Greenawalt, John W.; Lehninger, Albert L.

    1974-01-01

    The hepatopancreas of the adult male blue crab Callinectes sapidus in intermolt was found to contain substantial amounts of calcium, magnesium, and inorganic phosphorus, averaging about 260, 20, and 250 µg-atoms per g wet tissue, respectively, accounting for over 10% of the tissue dry weight. Electron microscopy of the intact tissue showed three qualitatively different granular structures having electron densities suggestive of high mineral content. After fractionation of the tissue using centrifugal techniques, almost 95% of the total mineral was found to reside in a heavy, nonmitochondrial particulate fraction(s). The bulk of the low-speed pellet consisted of relatively dense, roughly spherical granules 1–5 µm in diameter, which could be considerably purified by repeated suspension in water and low-speed sedimentation. In the electron microscope the isolated granules appeared basically similar to one of the three characteristic types of electron-dense granules seen in the intact tissue. Although the freshly isolated granules lost approximately 50% of their wet weight when dried at 105°C, only 10% more was lost upon dry ashing at 450°C, suggesting a fairly low content of organic material. Chemical analysis revealed calcium, magnesium, and inorganic phosphate at 5.7, 2.1, and 4.4 µg-atoms per mg dried granules, respectively, accounting for 69% of the dry weight of the fraction. By specific enzymatic assays, the freshly isolated granules were found to contain ATP, ADP, and AMP at levels of 0.13, 0.03, and 0.01 µmol/mg, or 8% of their total dry weight. The remainder of the total phosphorus contributed an additional 3%, whereas carbonate, citrate, oxalate, and protein each constituted no more than 1%. The mineral granules of the crab hepatopancreas appear to function as storage forms of calcium and phosphate during the intermolt period. This tissue appears promising as a model for study of the cellular events associated with biological calcification, since

  2. Sublethal Toxicity of Crude Oil Exposure in The Blue Crab, Callinectes sapidus, at Two Life History Stages.

    Science.gov (United States)

    Giltz, Sarah M; Taylor, Caz M

    2017-02-01

    The 2010 Deepwater Horizon oil spill occurred during peak spawning season for many Gulf of Mexico fish and invertebrates. Early life stages of important fishery species were at risk to encounter crude oil. In this study, we investigated the effect of crude oil exposure on two life stages of the blue crab (Callinectes sapidus). We tested the effects of oil exposure on the survival and growth of larval (pelagic) and juvenile (estuarine) blue crabs as well as the effects of oil on the molt frequency of juveniles. Larval crabs exposed to crude oil showed no discernable growth or mortality differences when compared to non-exposed controls. Juvenile crabs exposed to oil also showed no differences in size but exhibited increased intermolt duration (time between molts). Our study suggests that different life-stages may respond differently when exposed to crude oil and that oil exposure negatively affects growth rate of juvenile blue crabs.

  3. Toxicity of ozonated estuarine water to juvenile blue crabs (Callinectes sapidus) and Juvenile Atlantic menhaden (Brevoortia tyrannus)

    Energy Technology Data Exchange (ETDEWEB)

    Richardson, L.B.; Burton, D.T.

    1981-02-01

    Large quantitites of estuarine and marine water are treated with chlorine to prevent condenser system fouling at power plants. Chlorine and its residual by-products, however, are toxic to many forms of aquatic life. Ozone is one alternative oxidant which has proven to be an effective biocide and disinfectant in many fresh water applications. Ozonation of estuarine and marine waters, however, may produce residual compounds similar to those produced by chlorination. This study was initiated to provide baseline information on the toxicity of ozonated estuarine water to two representative estuarine species. The blue crab, Callinectes sapidus Rathbun, and the Atlantic menhaden, Brevoortia tyrannus Latrobe, were selected because of their wide distribution and commercial importance. The toxicity of ozone has been compared with chlorine toxicity data from the literature in an effort to examine possible similarities in toxicity.

  4. Detecting selection in the blue crab, Callinectes sapidus, using DNA sequence data from multiple nuclear protein-coding genes.

    Science.gov (United States)

    Yednock, Bree K; Neigel, Joseph E

    2014-01-01

    The identification of genes involved in the adaptive evolution of non-model organisms with uncharacterized genomes constitutes a major challenge. This study employed a rigorous and targeted candidate gene approach to test for positive selection on protein-coding genes of the blue crab, Callinectes sapidus. Four genes with putative roles in physiological adaptation to environmental stress were chosen as candidates. A fifth gene not expected to play a role in environmental adaptation was used as a control. Large samples (n>800) of DNA sequences from C. sapidus were used in tests of selective neutrality based on sequence polymorphisms. In combination with these, sequences from the congener C. similis were used in neutrality tests based on interspecific divergence. In multiple tests, significant departures from neutral expectations and indicative of positive selection were found for the candidate gene trehalose 6-phosphate synthase (tps). These departures could not be explained by any of the historical population expansion or bottleneck scenarios that were evaluated in coalescent simulations. Evidence was also found for balancing selection at ATP-synthase subunit 9 (atps) using a maximum likelihood version of the Hudson, Kreitmen, and Aguadé test, and positive selection favoring amino acid replacements within ATP/ADP translocase (ant) was detected using the McDonald-Kreitman test. In contrast, test statistics for the control gene, ribosomal protein L12 (rpl), which presumably has experienced the same demographic effects as the candidate loci, were not significantly different from neutral expectations and could readily be explained by demographic effects. Together, these findings demonstrate the utility of the candidate gene approach for investigating adaptation at the molecular level in a marine invertebrate for which extensive genomic resources are not available.

  5. Epidermal carbonic anhydrase activity and exoskeletal metal content during the molting cycle of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Calhoun, Stacy; Zou, Enmin

    2016-03-01

    During the crustacean molting cycle, the exoskeleton is first mineralized in postmolt and intermolt and then presumably demineralized in premolt in order for epidermal retraction to occur. The mineralization process calls for divalent metal ions, such as Ca(2+) and Mg(2+) , and bicarbonate ions whereas protons are necessary for dissolution of carbonate salts. Carbonic anhydrase (CA) has been suggested to be involved in exoskeletal mineralization by providing bicarbonate ions through catalyzing the reaction of carbon dioxide hydration. However, results of earlier studies on the role of epidermal CA in metal incorporation in crustacean exoskeleton are not consistent. This study was aimed to provide further evidence to support the notion that epidermal CA is involved in exoskeletal mineralization using the blue crab, Callinectes sapidus (Rathbun 1896), as the model crustacean. Significant increases first in calcium and magnesium then in manganese post-ecdysis indicate significant metal deposition during postmolt and intermolt. Significant positive correlation between calcium or magnesium content and epidermal CA activity in postmolt and intermolt constitutes evidence that CA is involved in the mineralization of the crustacean exoskeleton. Additionally, we proposed a hypothetical model to describe the role of epidermal CA in both mineralization and demineralization of the exoskeleton based on the results of epidermal CA activity and exoskeletal metal content during the molting cycle. Furthermore, we found that the pattern of epidermal CA activity during the molting cycle of C. sapidus is similar to that of ecdysteroids reported for the same species, suggesting that epidermal CA activity may be under control of the molting hormones. © 2016 Wiley Periodicals, Inc.

  6. The Involvement of Hemocyte Prophenoloxidase in the Shell-Hardening Process of the Blue Crab, Callinectes sapidus.

    Directory of Open Access Journals (Sweden)

    Javier V Alvarez

    Full Text Available Cuticular structures of arthropods undergo dramatic molt-related changes from being soft to becoming hard. The shell-hardening process of decapod crustaceans includes sclerotization and mineralization. Hemocyte PPO plays a central role in melanization and sclerotization particularly in wound healing in crustaceans. However, little is known about its role in the crustacean initial shell-hardening process. The earlier findings of the aggregation of heavily granulated hemocytes beneath the hypodermis during ecdysis imply that the hemocytes may be involved in the shell-hardening process. In order to determine if hemocytes and hemocyte PPO have a role in the shell-hardening of crustaceans, a knockdown study using specific CasPPO-hemo-dsRNA was carried out with juvenile blue crabs, Callinectes sapidus. Multiple injections of CasPPO-hemo-dsRNA reduce specifically the levels of CasPPO-hemo expression by 57% and PO activity by 54% in hemocyte lysate at the postmolt, while they have no effect on the total hemocyte numbers. Immunocytochemistry and flow cytometry analysis using a specific antiserum generated against CasPPO show granulocytes, semigranulocytes and hyaline cells as the cellular sources for PPO at the postmolt. Interestingly, the type of hemocytes, as the cellular sources of PPO, varies by molt stage. The granulocytes always contain PPO throughout the molt cycle. However, semigranulocytes and hyaline cells become CasPPO immune-positive only at early premolt and postmolt, indicating that PPO expression in these cells may be involved in the shell-hardening process of C. sapidus.

  7. The Involvement of Hemocyte Prophenoloxidase in the Shell-Hardening Process of the Blue Crab, Callinectes sapidus.

    Science.gov (United States)

    Alvarez, Javier V; Chung, J Sook

    2015-01-01

    Cuticular structures of arthropods undergo dramatic molt-related changes from being soft to becoming hard. The shell-hardening process of decapod crustaceans includes sclerotization and mineralization. Hemocyte PPO plays a central role in melanization and sclerotization particularly in wound healing in crustaceans. However, little is known about its role in the crustacean initial shell-hardening process. The earlier findings of the aggregation of heavily granulated hemocytes beneath the hypodermis during ecdysis imply that the hemocytes may be involved in the shell-hardening process. In order to determine if hemocytes and hemocyte PPO have a role in the shell-hardening of crustaceans, a knockdown study using specific CasPPO-hemo-dsRNA was carried out with juvenile blue crabs, Callinectes sapidus. Multiple injections of CasPPO-hemo-dsRNA reduce specifically the levels of CasPPO-hemo expression by 57% and PO activity by 54% in hemocyte lysate at the postmolt, while they have no effect on the total hemocyte numbers. Immunocytochemistry and flow cytometry analysis using a specific antiserum generated against CasPPO show granulocytes, semigranulocytes and hyaline cells as the cellular sources for PPO at the postmolt. Interestingly, the type of hemocytes, as the cellular sources of PPO, varies by molt stage. The granulocytes always contain PPO throughout the molt cycle. However, semigranulocytes and hyaline cells become CasPPO immune-positive only at early premolt and postmolt, indicating that PPO expression in these cells may be involved in the shell-hardening process of C. sapidus.

  8. Detecting selection in the blue crab, Callinectes sapidus, using DNA sequence data from multiple nuclear protein-coding genes.

    Directory of Open Access Journals (Sweden)

    Bree K Yednock

    Full Text Available The identification of genes involved in the adaptive evolution of non-model organisms with uncharacterized genomes constitutes a major challenge. This study employed a rigorous and targeted candidate gene approach to test for positive selection on protein-coding genes of the blue crab, Callinectes sapidus. Four genes with putative roles in physiological adaptation to environmental stress were chosen as candidates. A fifth gene not expected to play a role in environmental adaptation was used as a control. Large samples (n>800 of DNA sequences from C. sapidus were used in tests of selective neutrality based on sequence polymorphisms. In combination with these, sequences from the congener C. similis were used in neutrality tests based on interspecific divergence. In multiple tests, significant departures from neutral expectations and indicative of positive selection were found for the candidate gene trehalose 6-phosphate synthase (tps. These departures could not be explained by any of the historical population expansion or bottleneck scenarios that were evaluated in coalescent simulations. Evidence was also found for balancing selection at ATP-synthase subunit 9 (atps using a maximum likelihood version of the Hudson, Kreitmen, and Aguadé test, and positive selection favoring amino acid replacements within ATP/ADP translocase (ant was detected using the McDonald-Kreitman test. In contrast, test statistics for the control gene, ribosomal protein L12 (rpl, which presumably has experienced the same demographic effects as the candidate loci, were not significantly different from neutral expectations and could readily be explained by demographic effects. Together, these findings demonstrate the utility of the candidate gene approach for investigating adaptation at the molecular level in a marine invertebrate for which extensive genomic resources are not available.

  9. Occurence of the Asian shrimp Palaemon macrodactylus in the Southern bight of the North Sea, with a key to the Palaemonidae of North-western Europe (Crustacea: Decapoda: Caridea)

    NARCIS (Netherlands)

    d'Udekem d'Azoc, C.; Faasse, M.; Dumoulin, E.; Blauwe, de H.

    2005-01-01

    Het voorkomen van de rugstreepsteurgarnaal Palaemon macrodactylus in de zuidelijke bocht van de Noordzee, met een determinatiesleutel tot de Palaemonidae van Noordwest-Europa (Crustacea: Decapoda: Caridea) In 1999 werd de rugstreepsteurgarnaal Palaemon macrodactylus Rathbun, 1902, een soort

  10. Comparative analysis of the proximate and elemental composition of the blue crab Callinectes sapidus, the warty crab Eriphia verrucosa, and the edible crab Cancer pagurus

    OpenAIRE

    Zotti, Maurizio; Coco, Laura Del; Pascali, Sandra Angelica De; Migoni, Danilo; Vizzini, Salvatrice; Mancinelli, Giorgio; Fanizzi, Francesco Paolo

    2016-01-01

    The proximate composition and element contents of claw muscle tissue of Atlantic blue crabs (Callinectes sapidus) were compared with the native warty crab (Eriphia verrucosa) and the commercially edible crab (Cancer pagurus). The scope of the analysis was to profile the chemical characteristics and nutritive value of the three crab species. Elemental fingerprints showed significant inter-specific differences, whereas non-significant variations in the moisture and ash contents were observed. I...

  11. A second copper zinc superoxide dismutase (CuZnSOD) in the blue crab Callinectes sapidus: cloning and up-regulated expression in the hemocytes after immune challenge.

    Science.gov (United States)

    Sook Chung, J; Bachvaroff, T R; Trant, J; Place, A

    2012-01-01

    The full-length cDNA (1362 nucleotides, GenBank JF736621) encoding an extracellular copper zinc superoxide dismutase initially isolated from an EST library of the blue crab Callinectes sapidus was characterized using 3' RACE and named Cas-ecCuZnSOD-2. The open reading frame of Cas-ecCuZnSOD-2 contains 203 deduced amino acids with the conserved active catalytic center for copper and zinc binding and the post-translational modification at two putative N-glycosylation and nine phosphorylation sites. Overall, the deduced amino acids of Cas-ecCuZnSOD-2 shared only 35% sequence identity with that of Cas-ecCuZnSOD (GenBank AF264031) which was previously found in C. sapidus, while it showed ∼75% sequence identity to Scylla paramamosain ecCuZnSOD (GenBank FJ774661). The expression profile of Cas-ecCuZnSOD-2 and the other three C. sapidus SODs: ecCuZn, cytMn- and mitMn SODs was largely ubiquitous among the tested tissues obtained from a juvenile female at intermolt: brain, eyestalk ganglia, pericardial organs, and thoracic ganglia complex (nervous system); hepatopancreas (digestive system); heart, artery and hemocytes (circulatory system); gill and antennal gland (excretory system), hypodermis, and Y-organ (endocrine organ). Our study reports, for the first time in the crustaceans, expression analyses for all four Cas-SODs in hemocytes after immune challenges. Crabs challenged with lipopolysaccharides (LPS) injection had a remarkable induction of Cas-ecCuZnSOD-2 expression along with three other SODs in hemocytes, suggesting that Cas-SODs including Cas-ecCuZnSOD-2 are involved in the defense system, possibly innate immunity and immunocompetency of C. sapidus. Copyright © 2011 Elsevier Ltd. All rights reserved.

  12. Trehalose metabolism in the blue crab Callinectes sapidus: isolation of multiple structural cDNA isoforms of trehalose-6-phosphate synthase and their expression in muscles.

    Science.gov (United States)

    Shi, Q; Chung, J Sook

    2014-02-15

    Adult blue crab Callinectes sapidus exhibit behavioral and ecological dimorphisms: females migrating from the low salinity water to the high salinity area vs. males remaining in the same areas. The flesh basal muscle of the swimming paddle shows a dimorphic color pattern in that levator (Lev) and depressor (Dep) of females tend to be much darker than those of males, while both genders have the same light colored remoter (Rem) and promoter (Pro). The full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated from chela muscles of an adult female, C. sapidus. Two isoforms of the C. sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase (TPP) in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. In both males and females, the darker (Lev+Dep) muscles exhibit greater amounts of trehalose, TPS and trehalase activities than the light colored (Rem+Pro). The fact that adult females show higher levels of trehalase activity in the basal muscles and of glucose in Lev+Dep than those of adult males suggests that there may be a metabolic dimorphism. Moreover, the involvement of trehalose in energy metabolism that was examined under the condition of strenuous swimming activity mimicked in adult females demonstrates the intrinsic trehalose metabolism in Lev+Dep, which subsequently results in hemolymphatic hyperglycemia and hyperlactemia. Our data support that trehalose serves as an additional carbohydrate source of hemolymphatic hyperglycemia in this species. Behavioral and ecological dimorphisms of C. sapidus adults may be supported by a functional dimorphism in energy metabolism. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Genome sequence analysis of CsRV1, a pathogenic reovirus that infects the blue crab Callinectes sapidus across its trans-hemispheric range

    Directory of Open Access Journals (Sweden)

    Emily Maya Flowers

    2016-02-01

    Full Text Available The blue crab, Callinectes sapidus (Rathbun 1896, which is a commercially important trophic link in coastal ecosystems of the western Atlantic, is infected in both North and South America by C. sapidus Reovirus 1 (CsRV1, a double stranded RNA virus. The 12 genome segments of a North American strain of CsRV1 were sequenced using Ion Torrent technology. Putative functions could be assigned for 3 of the 13 proteins encoded in the genome, based on their similarity to proteins encoded in other reovirus genomes. Comparison of the CsRV1 RNA dependent RNA polymerase (RdRP sequence to genomes of other crab-infecting reoviruses shows that it is similar to the MCRV virus found in Scylla serrata, mud crab, and WX-2012 in Eriocheir sinensis, Chinese mitten crab, and supports the idea that there is a distinct Crabreo genus, different from Seadornavirus and Cardoreovirus, the two closest genera in the Reoviridae. A region of 98% nucleotide sequence identity between CsRV1 and the only available sequence of the P virus of Macropipus depurator suggests that these two viruses may be closely related. An 860 nucleotide region of the CsRV1 RdRP gene was amplified and sequenced from 15 infected crabs collected from across the geographic range of C. sapidus. Pairwise analysis of predicted protein sequences shows that CsRV1 strains in Brazil can be distinguished from those in North America based on conserved residues in this gene. The sequencing, annotation, and preliminary population metrics of the genome of CsRV1 should facilitate additional studies in diverse disciplines, including structure-function relationships of reovirus proteins, investigations into the evolution of the Reoviridae, and biogeographic research on the connectivity of C. sapidus populations across the Northern and Southern hemispheres.

  14. Genome Sequence Analysis of CsRV1: A Pathogenic Reovirus that Infects the Blue Crab Callinectes sapidus Across Its Trans-Hemispheric Range.

    Science.gov (United States)

    Flowers, Emily M; Bachvaroff, Tsvetan R; Warg, Janet V; Neill, John D; Killian, Mary L; Vinagre, Anapaula S; Brown, Shanai; Almeida, Andréa Santos E; Schott, Eric J

    2016-01-01

    The blue crab, Callinectes sapidus Rathbun, 1896, which is a commercially important trophic link in coastal ecosystems of the western Atlantic, is infected in both North and South America by C. sapidus Reovirus 1 (CsRV1), a double stranded RNA virus. The 12 genome segments of a North American strain of CsRV1 were sequenced using Ion Torrent technology. Putative functions could be assigned for 3 of the 13 proteins encoded in the genome, based on their similarity to proteins encoded in other reovirus genomes. Comparison of the CsRV1 RNA-dependent RNA polymerase (RdRP) sequence to genomes of other crab-infecting reoviruses shows that it is similar to the mud crab reovirus found in Scylla serrata and WX-2012 in Eriocheir sinensis, Chinese mitten crab, and supports the idea that there is a distinct "Crabreo" genus, different from Seadornavirus and Cardoreovirus, the two closest genera in the Reoviridae. A region of 98% nucleotide sequence identity between CsRV1 and the only available sequence of the P virus of Macropipus depurator suggests that these two viruses may be closely related. An 860 nucleotide region of the CsRV1 RdRP gene was amplified and sequenced from 15 infected crabs collected from across the geographic range of C. sapidus. Pairwise analysis of predicted protein sequences shows that CsRV1 strains in Brazil can be distinguished from those in North America based on conserved residues in this gene. The sequencing, annotation, and preliminary population metrics of the genome of CsRV1 should facilitate additional studies in diverse disciplines, including structure-function relationships of reovirus proteins, investigations into the evolution of the Reoviridae, and biogeographic research on the connectivity of C. sapidus populations across the Northern and Southern hemispheres.

  15. A new echiuran-associated snapping shrimp (Crustacea: Decapoda: Alpheidae) from the Indo-West Pacific.

    Science.gov (United States)

    Anker, Arthur; Komai, Tomoyuki; Marin, Ivan N

    2015-01-29

    Alpheus echiurophilus sp. nov. (Crustacea: Decapoda: Caridea: Alpheidae) is described based on material from Japan (Ryukyu Islands) and Vietnam (Nha Trang Bay); an additional, morphologically slightly different specimen from Madagascar (Nosy-Bé) is preliminarily referred to A. cf. echiurophilus sp. nov., awaiting collection of additional material and/or genetic comparison. All specimens of the new species were collected from burrows of thalassematid echiurans, either on intertidal and shallow subtidal sand-mud flats or in the mixed sand-gravel-rock intertidal. Alpheus echiurophilus sp. nov. belongs to the A. leviusculus species group, being morphologically closest to the Indo-West Pacific A. leviusculus Dana, 1852, A. hululensis Coutière, 1905, A. ladronis Banner, 1956, and the western Atlantic A. zimmermani Anker, 2007. The new species can be separated from all of them by a combination of morphological characters and also appears to have a diagnostic colouration.

  16. Estructura del aparato reproductor masculino de Uca uruguayensis (Decapoda, Ocypodidae Structure of the male reproductive tract of Uca uruguayensis (Decapoda, Ocypodidae

    Directory of Open Access Journals (Sweden)

    Elena Irene Cuartas

    Full Text Available El objetivo de este trabajo es describir las estructuras del sistema reproductor de machos de Uca uruguayensis Nobili, 1901 y estudiar los cambios relacionados con la madurez sexual. Se trabajó con observación estereoscópica de material fresco, técnica histológica de rutina y observaciones en Microscopio Electrónico de Barrido. Se incluyó la caracterización de los gonopodos 1 (G1 y 2 (G2. El tracto reproductor del macho se compone de un par de testículos (T, un vaso deferente (VD tubular y sinuoso y una ampolla terminal (AT. El VD tiene tres secciones, la anterior (VDA, la media (VDM y la posterior (VDP. El VDA y VDM están conformadas por un epitelio simple de células cúbicas. El epitelio del VDP es columnar y con núcleos basales y alongados. El diseño tubular se modifica al ocuparse el lumen del VDP con líquido espermático durante el verano y la musculatura circular se hace más evidente. La porción terminal del VDP se ensancha formando una ampolla (AT que comprende cuatro cámaras interconectadas. Todas las estructuras están rodeadas de una capa de tejido conectivo de poco espesor. Se identifican las modificaciones observadas en la histología de T y VD, definiendo como mas relevantes las observadas desde el mes de noviembre hasta marzo. Estas modificaciones sugieren que U. uruguayensis tiene, en la localidad estudiada, una única estación reproductiva durante el verano en esta latitud. La AT, tal como es descripta, es una estructura que hasta el momento no ha sido mencionada para los Brachyura.The structure of the male reproductive tract was described in Uca uruguayensis Nobili, 1901, by using histological methods, scanning electron microscopy techniques, and stereoscopic observations of fresh material. The aim of this work was to establish the functional changes associated with sexual maturation. The morphology of the first (G1 and second (G2 pair of gonopods was described. The male reproductive tract consists of paired

  17. LAMBRACHAEUS RAM/FER ALCOCK, A RARE SPIDER CRAB ...

    African Journals Online (AJOL)

    LAMBRACHAEUS RAM/FER ALCOCK, A RARE SPIDER CRAB FROM THE. EAST COAST OF SOUTHERN AFRICA (DECAPODA, BRACHYURA,. MAJIDAE). BRIAN KENS LEY. South African Museum. Cape Town. Accepted: May 1977. ABSTRACT. Lambrachaew rami/er Alcock, previously known only from a single record ...

  18. Sesarma (Sesarma) cerberus, a new cavernicolous crab from Amboina

    NARCIS (Netherlands)

    Holthuis, L.B.

    1964-01-01

    Though the number of species of macrurous Decapoda known from subterranean waters is rather extensive, there are surprisingly few species of crabs that have been found in that habitat. Wolf (1934: 105, 106) listed only four species of Brachyura, three of which are epigeal forms, which had only

  19. Biodegradation of vegetable and agrowastes by Pleurotus sapidus: a novel strategy to produce mushroom with enhanced yield and nutrition.

    Science.gov (United States)

    Singh, M P; Singh, V K

    2012-12-22

    Edible oyster mushroom Pleurotus sapidus was cultivated, on pea pod shell, cauliflower leaves, radish leaves, brassica straw in various combinations of paddy straw. The mushroom failed to grow on these vegetable wastes separately. However, it grew very well on these vegetable wastes when mixed with various combinations of paddy straw as substrate. Total yield and biological efficiency of the mushroom cultivated on substrate containing 20% and 30% vegetable wastes mixed with 70% and 80% (w/w) of paddy straw was found to be better, when compared with yield and efficiency obtained with paddy straw alone (100%). The protein content in the fruit bodies was found to be higher in the mushroom grown on paddy straw mixed with vegetable wastes than that obtained with paddy straw alone. Similarly, six amino acids (Leu, Ile, Val, Thr, Met and Phe) showed a significant increase when the mushroom was grown on a mixed substrate containing both vegetable wastes and paddy straw. On the contrary, the total sugar and reducing sugar content declined in the mushroom grown on the mixture of paddy straw and other wastes, when compared with the results obtained with paddy straw alone.

  20. Effects of acidifying ocean conditions on growth and survival of two life stages of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Giltz, S.; Taylor, C.

    2016-02-01

    Blue crabs, Callinectes sapidus, begin their larval phase offshore and circulate for approximately 30 days before settling near shore. As crabs transition to the juvenile stage, they move into coastal or estuarine environments characterized by lower salinity. Presently the average pH of the ocean is 8.1, 30% down from the beginning of the industrial revolution and is forecasted to drop to 7.8 by 2100. Decreasing pH causes dissolution of calcium carbonate shells, but the overall effects on crustaceans, such as blue crabs, are unknown. This study investigated the effect of a lower pH environment on the growth, survival, carapace hardness and molt frequency of larval and juvenile blue crabs in the Northern Gulf of Mexico. Larval crabs showed delayed growth under low pH (7.8) conditions compared to crabs in a control (present day) pH (8.1) environment. Population crashes (complete mortality) were experienced in 55% of the low pH aquaria but not in any of the control aquaria, suggesting that acidification poses a mortality risk. Under low pH conditions the intermolt duration decreased in juveniles, but the body length and weight did not differ from crabs raised in the control pH. Larvae (in tanks that did not crash) and juveniles did not experience increased mortality from a lower pH, but there do appear to be sublethal effects on growth and molting that differ between life history stages.

  1. Species profiles: Life histories and environmental requirements of coastal fishes and invertebrates (Gulf of Mexico): Blue crab. [Callinectes sapidus

    Energy Technology Data Exchange (ETDEWEB)

    Perry, H.M.; McIlwain, T.D.

    1986-06-01

    The blue crab, Callinectes sapidus, is common in tidal marsh estuaries and coastal waters of the Gulf of Mexico, occupying a variety of habitats depending upon the physiological requirements of each particular stage in its life history. Spawning occurs from spring through fall in high salinity estuarine and/or coastal waters. Development through the 7 zoeal stages requires approximately 31 days and occurs offshore. The megalopal stage is usually completed within a week. Recruitment to the estuary occurs during the megalopal stage. Molt to the first crab takes place within the estuary. Juveniles exhibit wide seasonal and areal distribution. Growth is rapid and blue crabs in the Gulf of Mexico may reach maturity within a year. Factors affecting growth and survival include food availability, predation, substratum, available habitat, temperature, salinity and pollutants. Blue crabs do not conform to specific trophic levels and are characterized as opportunistic benthic omnivores. Their diverse feeding habits and their importance as prey species for a variety of organisms make them an integral part of coastal ecosystems.

  2. Acute toxicity, histopathology, and bioconcentration-retention studies with No. 2 fuel oil and the blue crab, Callinectes sapidus Rathbun

    Energy Technology Data Exchange (ETDEWEB)

    Melzian, B.D.

    1982-01-01

    Adult male blue crabs (Callinectes sapidus Rathbun) were exposed in a flow-through system to five concentrations, nominally 0.0 (control), 1.0, 5.0, 10.0, or 20.0 ppm of the water accommodated fraction (WAF) of No. 2 fuel oil. The 96-hour LC/sub 5//sup 0/ was 14.1 ppm. All crabs in the 5.0, 10.0, and 20.0 ppm tanks exhibited abnormal behavior prior to death including periodic beating of the maxillipedal flagella, hyperactivity, ataxia, and equilibrium loss. The ability of adult male and female blue crabs to bioconcentrate and retain No. 2 fuel oil compounds was examined after crabs were exposed for two weeks or 30-35 days in flow-through systems to three sublethal No. 2 fuel oil WAF concentrations, nominally 0.00 (control), 0.01, or 1.0 ppm. Gill, hepatopancreas, and muscle tissues were analyzed by gas chromatography/mass spectrometry. Trace amounts of fuel oil hydrocarbons were detected in gill and hepatopancreas at 0.01 ppm exposures. All tissues bioconcentrated fuel oil compounds during 1.0 ppm WAF exposure periods, and with the exception of the gills, little depuration or metabolism of the bioconcentrated compounds occurred during a period of 30 days in clean, running seawater.

  3. Accumulation and retention of No. 2 fuel-oil compounds in the blue crab, Callinectes sapidus' rathbun

    Energy Technology Data Exchange (ETDEWEB)

    Melzian, B.D.; Lake, J.

    1987-01-01

    In order to determine if Blue Crabs (Callinectes sapidus) can bioaccumulate and retain complex mixtures of petroleum hydrocarbons, adult crabs were exposed for 14 days or 30-35 days in continuous flow-through seawater systems to three sublethal concentrations, nominally 0.00 (control), 0.01, or 1.0 ppm (mg/liter), of the water-accomodated fraction (WAF) of Number (No.) 2 fuel oil. Crabs exposed for 14 days were subsequently exposed to clean running seawater for a 30 day depuration period. Gill, hepatopancreas, and muscle tissue samples were collected from control, WAF exposed, and depurated crabs and analyzed by gas chromatography and/or gas chomatography-mass spectrometry. No. 2 fuel-oil compounds were not detected in any of the tissues of the control crabs. Trace amounts of fuel-oil compounds were detected in gill and hepatopancreas tissues collected from crabs exposed to the 0.01 ppm WAF and no fuel-oil compounds were detected in muscle tissues. All tissues of crabs exposed to the 1.0 ppm WAF accumulated No. 2 fuel-oil compounds and considerable amounts remained in hepatopancreas and gill tissues following depuration for 30 days in clean seawater.

  4. Grooming behaviors and gill fouling in the commercially important blue crab (Callinectes sapidus and stone crab (Menippe mercenaria

    Directory of Open Access Journals (Sweden)

    Jen L. Wortham

    2017-10-01

    Full Text Available Abstract Grooming behaviors reduce fouling of body regions. In decapods, grooming time budgets, body regions groomed, and grooming appendages are known in several species; however, little data exists on brachyuran crabs. In this study, grooming behaviors of two commercially important crabs were documented (blue crabs: Callinectes sapidus Rathbun, 1896; stone crabs: Menippe mercenaria Say, 1818. These crabs are harvested by fishermen and knowing their grooming behaviors is valuable, as clean crabs are preferred by consumers and the stone crab fishery consequence of removing one cheliped to grooming behaviors is unknown. Crabs were observed individually and agonistically to determine how grooming behaviors vary in the presence of another conspecific. Both species frequently use their maxillipeds and groom, with the gills being cleaned by epipods. Respiratory and sensory structures were groomed frequently in both species. Removal of a grooming appendage resulted in higher fouling levels in the gills, indicating that grooming behaviors do remove fouling. Overall, stone crabs had a larger individual time budget for grooming, but agonistic grooming time budgets were similar. Stone crab chelipeds are used in grooming, especially cleaning the other cheliped. The chelipeds are not the main grooming appendage; however, implications of losing one cheliped may have large impacts.

  5. A new PCR-based method shows that blue crabs (Callinectes sapidus (Rathbun)) consume winter flounder (Pseudopleuronectes americanus (Walbaum)).

    Science.gov (United States)

    Collier, Jackie L; Fitzgerald, Sean P; Hice, Lyndie A; Frisk, Michael G; McElroy, Anne E

    2014-01-01

    Winter flounder (Pseudopleuronectes americanus) once supported robust commercial and recreational fisheries in the New York (USA) region, but since the 1990s populations have been in decline. Available data show that settlement of young-of-the-year winter flounder has not declined as sharply as adult abundance, suggesting that juveniles are experiencing higher mortality following settlement. The recent increase of blue crab (Callinectes sapidus) abundance in the New York region raises the possibility that new sources of predation may be contributing to juvenile winter flounder mortality. To investigate this possibility we developed and validated a method to specifically detect winter flounder mitochondrial control region DNA sequences in the gut contents of blue crabs. A survey of 55 crabs collected from Shinnecock Bay (along the south shore of Long Island, New York) in July, August, and September of 2011 showed that 12 of 42 blue crabs (28.6%) from which PCR-amplifiable DNA was recovered had consumed winter flounder in the wild, empirically supporting the trophic link between these species that has been widely speculated to exist. This technique overcomes difficulties with visual identification of the often unrecognizable gut contents of decapod crustaceans, and modifications of this approach offer valuable tools to more broadly address their feeding habits on a wide variety of species.

  6. A Multifaceted Mass Spectrometric Method to Probe Feeding Related Neuropeptide Changes in Callinectes sapidus and Carcinus maenas.

    Science.gov (United States)

    Zhang, Yuzhuo; DeLaney, Kellen; Hui, Limei; Wang, Junhua; Sturm, Robert M; Li, Lingjun

    2018-02-12

    Food intake is regulated by various neuromodulators, including numerous neuropeptides. However, it remains elusive at the molecular and cellular level as to how these important chemicals regulate internal processes and which regions of the neuronal organs are responsible for regulating the behavior. Here we report a comparative neuropeptidomic analysis of the brain and pericardial organ (PO) in response to feeding in two well-studied crustacean physiology model organisms, Callinectes sapidus and Carcinus maenas, using mass spectrometry (MS) techniques. A multifaceted MS-based approach has been developed to obtain complementary information on the expression changes of a large array of neuropeptides in the brain and PO. The method employs stable isotope labeling of brain and PO extracts for relative MS quantitation, capillary electrophoresis (CE)-MS for fractionation and high-specificity analysis, and mass spectrometric imaging (MSI) for in-situ molecular mapping of peptides. A number of neuropeptides, including RFamides, B-type allatostatins (AST-B), RYamides, and orcokinins exhibit significant changes in abundance after feeding in this investigation. Peptides from the AST-B family found in PO tissue were shown to have both altered expression and localization changes after feeding, indicating that they may be a class of vital neuropeptide regulators involved in feeding behavior. Graphical Abstract ᅟ.

  7. Mercury, lead, and cadmium in blue crabs, Callinectes sapidus, from the Atlantic coast of Florida, USA: a multipredator approach.

    Science.gov (United States)

    Adams, Douglas H; Engel, Marc E

    2014-04-01

    Blue crabs, Callinectes sapidus, from the Atlantic coast of Florida were analyzed for total mercury, methylmercury, lead, and cadmium. Paired samples of two tissue types were analyzed for each crab, (1) muscle tissue (cheliped and body muscles) and (2) whole-body tissue (all organs, muscle tissue and connective tissue), for evaluation of the concentration of metals available to human consumers as well as estuarine predators. There were clear patterns of tissue-specific partitioning for each metal. Total mercury was significantly greater in muscle tissue (mean=0.078 µg/g) than in whole-body tissue (mean=0.055 µg/g). Conversely, whole-body concentrations of lead and cadmium (means=0.131 and 0.079 µg/g, respectively) were significantly greater than concentrations in muscle (means=0.02 and 0.029 µg/g, respectively). There were no significant correlations between any metal contaminant and crab size. Cadmium levels were significantly greater in the muscle tissue of females, but, no other sex-related differences were seen for other metals or tissue types. Methylmercury composed 93-100% of the total mercury in tissues. Compared to previous blue crab studies from different regions of the United States, mean concentrations of mercury, lead, and cadmium were relatively low, although isolated groups or individual blue crabs accumulated high metal concentrations. © 2013 Published by Elsevier Inc.

  8. Detailed spectroscopic study of the role of Br and Sr in coloured parts of the Callinectes sapidus crab claw.

    Science.gov (United States)

    Katsikini, M

    2016-07-01

    The exoskeleton of crustaceans consists mainly of calcium carbonate (CaCO3) minerals and in many cases exhibits vivid colouration due to the presence of proteins rich in carotenoid chromophores. The exposure of aquatic animals in sea water results often in the incorporation of trace elements in their exoskeleton. The bonding configuration of Br and Sr trace elements in regions with different staining (white, orange and blue) of the exoskeleton of the Callinectes sapidus in crab claw are systematically investigated by a number of complementary spectroscopic techniques, including X-ray absorption fine structure spectroscopy (EXAFS), X-ray fluorescence, Raman and visible light reflectivity spectroscopies. It is found that Sr substitutes for Ca and the Sr/Ca ratio is constant along the claw. In the orange region that includes the claw fingers, CaCO3 adopts a calcite-like structure, whereas in the blue and white regions, located in the palm of the claw, an aragonite-like structure dominates. On the other hand, Br, present only in the blue and orange stained parts of the claw, is bound to phenyl and/or phenol rings of amino acid residues, most probably to phenylalanine and/or tyrosine, of the chromophore protein. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Application of Artificial Neural Networks (ANNs for Weight Predictions of Blue Crabs (Callinectes sapidus RATHBUN, 1896 Using Predictor Variables

    Directory of Open Access Journals (Sweden)

    C. TURELI BILEN

    2011-10-01

    Full Text Available An evaluation of the performance of artificial networks (ANNs to estimate the weights of blue crab (Callinectes sapidus catches in Yumurtalık Cove (Iskenderun Bay that uses measured predictor variables is presented, including carapace width (CW, sex (male, female and female with eggs, and sampling month. Blue crabs (n=410 were collected each month between 15 September 1996 and 15 May 1998. Sex, CW, and sampling month were used and specified in the input layer of the network. The weights of the blue crabs were utilized in the output layer of the network. A multi-layer perception architecture model was used and was calibrated with the Levenberg Marguardt (LM algorithm. Finally, the values were determined by the ANN model using the actual data. The mean square error (MSE was measured as 3.3, and the best results had a correlation coefficient (R of 0.93. We compared the predictive capacity of the general linear model (GLM versus the Artificial Neural Network model (ANN for the estimation of the weights of blue crabs from independent field data. The results indicated the higher performance capacity of the ANN to predict weights compared to the GLM (R=0.97 vs. R=0.95, raw variable when evaluated against independent field data.

  10. Conservation in the first internal transcribed spacer (ITS1) region of Hematodinium perezi (genotype III) from Callinectes sapidus .

    Science.gov (United States)

    Pagenkopp Lohan, Katrina M; Small, Hamish J; Shields, Jeffrey D; Place, Allen R; Reece, Kimberly S

    2013-03-13

    Hematodinium spp. infections have been reported from blue crabs Callinectes sapidus in high-salinity waters of the USA from New Jersey to Texas. Recently, H. perezi (genotype III) has been proposed as the parasite species and genotype infecting blue crabs from Virginia; however, it is unknown whether this same genotype is present in blue crabs from other locations. To address this question, we collected 317 blue crabs from Massachusetts, Virginia, Georgia, Florida, Louisiana, and Texas to test for the presence of H. perezi (III) using a specific PCR assay targeting the first internal transcribed spacer (ITS1) region of the ribosomal RNA gene complex. To examine the genetic variation within H. perezi (III), ITS1 region sequences from the parasite in blue crabs from multiple locations were compared to each other and to those of H. perezi (III) found in alternate hosts from Virginia. In total, 34 distinct ITS1 sequence variants of the parasite were identified from blue crabs alone, and 38 distinct variants were identified when alternate hosts were included. However, a single ITS1 sequence variant appeared in all geographic regions and hosts, and also in blue crabs sampled from a previous study. The high similarity among all the ITS1 region sequences examined (>98%) and the observation of a single variant found throughout a large geographic range, strongly suggests that a single species and genotype of Hematodinium, specifically H. perezi (III), infects blue crabs from Virginia to Texas and multiple alternate host species in Virginia.

  11. A new PCR-based method shows that blue crabs (Callinectes sapidus (Rathbun consume winter flounder (Pseudopleuronectes americanus (Walbaum.

    Directory of Open Access Journals (Sweden)

    Jackie L Collier

    Full Text Available Winter flounder (Pseudopleuronectes americanus once supported robust commercial and recreational fisheries in the New York (USA region, but since the 1990s populations have been in decline. Available data show that settlement of young-of-the-year winter flounder has not declined as sharply as adult abundance, suggesting that juveniles are experiencing higher mortality following settlement. The recent increase of blue crab (Callinectes sapidus abundance in the New York region raises the possibility that new sources of predation may be contributing to juvenile winter flounder mortality. To investigate this possibility we developed and validated a method to specifically detect winter flounder mitochondrial control region DNA sequences in the gut contents of blue crabs. A survey of 55 crabs collected from Shinnecock Bay (along the south shore of Long Island, New York in July, August, and September of 2011 showed that 12 of 42 blue crabs (28.6% from which PCR-amplifiable DNA was recovered had consumed winter flounder in the wild, empirically supporting the trophic link between these species that has been widely speculated to exist. This technique overcomes difficulties with visual identification of the often unrecognizable gut contents of decapod crustaceans, and modifications of this approach offer valuable tools to more broadly address their feeding habits on a wide variety of species.

  12. In vitro culture and developmental cycle of the parasitic dinoflagellate Hematodinium sp. from the blue crab Callinectes sapidus.

    Science.gov (United States)

    Li, Caiwen; Miller, Terrence L; Small, Hamish J; Shields, Jeffrey D

    2011-12-01

    Hematodinium is a genus of parasitic dinoflagellates whose species have caused significant mortalities in marine crustacean fisheries worldwide. A species of Hematodinium infects the blue crab, Callinectes sapidus on the eastern seaboard of the USA. The mode of transmission of the parasite in blue crabs is unknown. We established several continuous in vitro cultures of Hematodinium sp. isolated from the haemolymph of infected blue crabs. One isolate has been continuously maintained in our laboratory through serial subcultivation for over 12 months, and is capable of infecting new hosts when inoculated into healthy crabs. Cells of the parasite undergo characteristic developmental changes in vitro consistent with the identifiable stages of Hematodinium sp.: filamentous trophonts, amoeboid trophonts, arachnoid trophonts and sporonts, sporoblasts, prespores and dinospores (macrospores and microspores). Additionally, we describe an unusual shunt in the life cycle wherein presumptive schizonts derived from arachnoid sporonts developed into filamentous and arachnoid trophonts that can then initiate arachnoid sporonts in new cultures. This may explain the rapid proliferation of the parasite in blue crab hosts. We also found that temperature and light intensity affected the growth and development of the parasite in vitro.

  13. Physicochemical properties of double-stranded RNA used to discover a reo-like virus from blue crab Callinectes sapidus.

    Science.gov (United States)

    Bowers, Holly A; Messick, Gretchen A; Hanif, Ammar; Jagus, Rosemary; Carrion, Lee; Zmora, Oded; Schott, Eric J

    2010-12-07

    Mortality among blue crab Callinectes sapidus in soft shell production facilities is typically 25% or greater. The harvest, handling, and husbandry practices of soft shell crab production have the potential to spread or exacerbate infectious crab diseases. To investigate the possible role of viruses in soft shell crab mortalities, we took advantage of the physicochemical properties of double-stranded RNA (dsRNA) to isolate a putative virus genome. Further characterization confirmed the presence of a reo-like virus that possesses 12 dsRNA genome segments. The virus was present in >50% of dead or dying soft shell crabs, but fewer than 5% of healthy hard crabs. Injection of the virus caused mortality and resulted in the appearance of viral RNA and virus inclusions in hemocytes. The genome of the virus was partially sequenced and the information used to develop a reverse transcription polymerase chain reaction (RT-PCR) assay that is able to detect the virus genome in as little as 7.5 pg of total RNA. The molecular tools developed during this study will allow us to quantify prevalence of the blue crab reo-like virus in captive (soft shell facilities, aquaculture operations) and wild populations and facilitate understanding of the role this virus has in blue crab life history.

  14. Evolution and phylogeny of the mud shrimps (Crustacea: Decapoda) revealed from complete mitochondrial genomes.

    Science.gov (United States)

    Lin, Feng-Jiau; Liu, Yuan; Sha, Zhongli; Tsang, Ling Ming; Chu, Ka Hou; Chan, Tin-Yam; Liu, Ruiyu; Cui, Zhaoxia

    2012-11-16

    The evolutionary history and relationships of the mud shrimps (Crustacea: Decapoda: Gebiidea and Axiidea) are contentious, with previous attempts revealing mixed results. The mud shrimps were once classified in the infraorder Thalassinidea. Recent molecular phylogenetic analyses, however, suggest separation of the group into two individual infraorders, Gebiidea and Axiidea. Mitochondrial (mt) genome sequence and structure can be especially powerful in resolving higher systematic relationships that may offer new insights into the phylogeny of the mud shrimps and the other decapod infraorders, and test the hypothesis of dividing the mud shrimps into two infraorders. We present the complete mitochondrial genome sequences of five mud shrimps, Austinogebia edulis, Upogebia major, Thalassina kelanang (Gebiidea), Nihonotrypaea thermophilus and Neaxius glyptocercus (Axiidea). All five genomes encode a standard set of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a putative control region. Except for T. kelanang, mud shrimp mitochondrial genomes exhibited rearrangements and novel patterns compared to the pancrustacean ground pattern. Each of the two Gebiidea species (A. edulis and U. major) and two Axiidea species (N. glyptocercus and N. thermophiles) share unique gene order specific to their infraorders and analyses further suggest these two derived gene orders have evolved independently. Phylogenetic analyses based on the concatenated nucleotide and amino acid sequences of 13 protein-coding genes indicate the possible polyphyly of mud shrimps, supporting the division of the group into two infraorders. However, the infraordinal relationships among the Gebiidea and Axiidea, and other reptants are poorly resolved. The inclusion of mt genome from more taxa, in particular the reptant infraorders Polychelida and Glypheidea is required in further analysis. Phylogenetic analyses on the mt genome sequences and the distinct gene orders provide further

  15. Evolution and phylogeny of the mud shrimps (Crustacea: Decapoda) revealed from complete mitochondrial genomes

    Science.gov (United States)

    2012-01-01

    Background The evolutionary history and relationships of the mud shrimps (Crustacea: Decapoda: Gebiidea and Axiidea) are contentious, with previous attempts revealing mixed results. The mud shrimps were once classified in the infraorder Thalassinidea. Recent molecular phylogenetic analyses, however, suggest separation of the group into two individual infraorders, Gebiidea and Axiidea. Mitochondrial (mt) genome sequence and structure can be especially powerful in resolving higher systematic relationships that may offer new insights into the phylogeny of the mud shrimps and the other decapod infraorders, and test the hypothesis of dividing the mud shrimps into two infraorders. Results We present the complete mitochondrial genome sequences of five mud shrimps, Austinogebia edulis, Upogebia major, Thalassina kelanang (Gebiidea), Nihonotrypaea thermophilus and Neaxius glyptocercus (Axiidea). All five genomes encode a standard set of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a putative control region. Except for T. kelanang, mud shrimp mitochondrial genomes exhibited rearrangements and novel patterns compared to the pancrustacean ground pattern. Each of the two Gebiidea species (A. edulis and U. major) and two Axiidea species (N. glyptocercus and N. thermophiles) share unique gene order specific to their infraorders and analyses further suggest these two derived gene orders have evolved independently. Phylogenetic analyses based on the concatenated nucleotide and amino acid sequences of 13 protein-coding genes indicate the possible polyphyly of mud shrimps, supporting the division of the group into two infraorders. However, the infraordinal relationships among the Gebiidea and Axiidea, and other reptants are poorly resolved. The inclusion of mt genome from more taxa, in particular the reptant infraorders Polychelida and Glypheidea is required in further analysis. Conclusions Phylogenetic analyses on the mt genome sequences and the

  16. The effects of temperature and salinity on larval development of Armases rubripes Rathbun, 1897 (Brachyura, Grapsoidea, Sesarmidae), and the southern limit of its geographical distribution

    Science.gov (United States)

    Luppi, T. A.; Spivak, E. D.; Bas, C. C.

    2003-11-01

    The southernmost stable population of Armases (= Metasesarma) rubripes (Brachyura, Grapsoidea, Sesarmidae) is found in Montevideo, Uruguay, in the northeastern coast of the Rio de la Plata estuary. Isolated individuals have seldom been collected in the southwestern coast of this huge estuary. Since pelagic larvae are primarily responsible for the dispersion of A. rubripes, and crab larvae are generally less tolerant than adults to extreme environmental conditions, we tested the survival and intermolt period of zoeae in different combinations of salinity (10, 20, and 30 psu) and temperature (16 and 20 °C). These salinity and temperature conditions are usually found in the estuary and adjacent waters during spring and summer. We found that the survival rate of the larvae increased and they developed faster at 30 psu and 20 °C. It is unlikely that larvae from the Montevideo population of A. rubripes could be transported south and westward due to the hydrographic conditions of the region. The Rio de la Plata seems to function as a biogeographic barrier to A. rubripes, and probably to other crab species as well. However, this barrier is not absolute: it hinders, but does not entirely prevent, the dispersal of larvae.

  17. The effects of fipronil and the photodegradation product fipronil desulfinyl on growth and gene expression in juvenile blue crabs, Callinectes sapidus, at different salinities

    Science.gov (United States)

    Goff, Andrew D.; Saranjampour, Parichehr; Ryan, Lauren M.; Hladik, Michelle; Covi, Joseph A.; Armbrust, Kevin L.; Brander, Susanne M.

    2017-01-01

    Endocrine disrupting compounds (EDCs) are now widely established to be present in the environment at concentrations capable of affecting wild organisms. Although many studies have been conducted in fish, less is known about effects in invertebrates such as decapod crustaceans. Decapods are exposed to low concentrations of EDCs that may cause infertility, decreased growth, and developmental abnormalities. The objective herein was to evaluate effects of fipronil and its photodegradation product fipronil desulfinyl. Fipronil desulfinyl was detected in the eggs of the decapod Callinectes sapidus sampled off the coast of South Carolina. As such, to examine specific effects on C. sapidus exposed in early life, we exposed laboratory-reared juveniles to fipronil and fipronil desulfinyl for 96 hours at three nominal concentrations (0.01, 0.1, 0.5 μg/L) and two different salinities (10, 30 ppt). The size of individual crabs (weight, carapace width) and the expression of several genes critical to growth and reproduction were evaluated. Exposure to fipronil and fipronil desulfinyl resulted in significant size increases in all treatments compared to controls. Levels of expression for vitellogenin (Vtg), an egg yolk precursor, and the ecdysone receptor (EcR), which binds to ecdysteroids that control molting, were inversely correlated with increasing fipronil and fipronil desulfinyl concentrations. Effects on overall growth and on the expression of EcR and Vtg differ depending on the exposure salinity. The solubility of fipronil is demonstrated to decrease considerably at higher salinities. This suggests that fipronil and its photodegradation products may be more bioavailable to benthic organisms as salinity increases, as more chemical would partition to tissues. Our findings suggest that endocrine disruption is occurring through alterations to gene expression in C. sapidus populations exposed to environmental levels of fipronil, and that effects may be dependent upon the

  18. Trophic flexibility of the Atlantic blue crab Callinectes sapidus in invaded coastal systems of the Apulia region (SE Italy): A stable isotope analysis

    Science.gov (United States)

    Mancinelli, Giorgio; Teresa Guerra, Maria; Alujević, Karla; Raho, Davide; Zotti, Maurizio; Vizzini, Salvatrice

    2017-11-01

    The Atlantic blue crab Callinectes sapidus is recognized as an Invasive Alien Species in the Mediterranean Sea. However, its trophic role and feeding flexibility in invaded benthic food webs have been addressed only recently. Here, field samplings were conducted in winter and summer in five coastal systems of the Apulia region (SE Italy), three located on the Ionian Sea (Mar Piccolo, Torre Colimena, and Spunderati) and two on the Adriatic Sea (Acquatina and Alimini Grande). Captured blue crabs were weighed and had their δ13C and δ15N isotopic signatures measured; their trophic level (TL) was estimated using the mussel Mytilus galloprovincialis as isotopic baseline. C. sapidus abundances varied greatly across systems and seasons, and in Adriatic systems the species was not collected in winter. Trophic levels showed significant spatial and temporal variations, although with no general pattern. In winter, the Mar Piccolo population showed the highest TL values; the lowest estimates were in Torre Colimena and Spunderati, where crabs showed δ13C signatures significantly higher than mussels, suggesting the contribution of 13C-enriched plant material in the diet. In summer, with the exception of the Mar Piccolo, Ionian populations increased their trophic level; both Adriatic populations were characterized by the lowest TL estimates. The analysis performed at the individual scale further indicated body weight-related changes in trophic level. For the Torre Colimena population, in particular, a hump-shaped pattern was observed in both seasons. The present study highlighted a considerable spatial and temporal trophic flexibility of C. sapidus at the population scale, while at the individual scale size-related shifts in trophic level were observed. The ability of the blue crab to vary its energy sources in relation with season, local environmental conditions, and ontogenetic stage is emphasized, suggesting that it may represent a key determinant of its invasion success.

  19. The effects of fipronil and the photodegradation product fipronil desulfinyl on growth and gene expression in juvenile blue crabs, Callinectes sapidus, at different salinities.

    Science.gov (United States)

    Goff, Andrew D; Saranjampour, Parichehr; Ryan, Lauren M; Hladik, Michelle L; Covi, Joseph A; Armbrust, Kevin L; Brander, Susanne M

    2017-05-01

    Endocrine disrupting compounds (EDCs) are now widely established to be present in the environment at concentrations capable of affecting wild organisms. Although many studies have been conducted in fish, less is known about effects in invertebrates such as decapod crustaceans. Decapods are exposed to low concentrations of EDCs that may cause infertility, decreased growth, and developmental abnormalities. The objective herein was to evaluate effects of fipronil and its photodegradation product fipronil desulfinyl. Fipronil desulfinyl was detected in the eggs of the decapod Callinectes sapidus sampled off the coast of South Carolina. As such, to examine specific effects on C. sapidus exposed in early life, we exposed laboratory-reared juveniles to fipronil and fipronil desulfinyl for 96h at three nominal concentrations (0.01, 0.1, 0.5μg/l) and two different salinities (10, 30ppt). The size of individual crabs (weight, carapace width) and the expression of several genes critical to growth and reproduction were evaluated. Exposure to fipronil and fipronil desulfinyl resulted in significant size increases in all treatments compared to controls. Levels of expression for vitellogenin (Vtg), an egg yolk precursor, and the ecdysone receptor (EcR), which binds to ecdysteroids that control molting, were inversely correlated with increasing fipronil and fipronil desulfinyl concentrations. Effects on overall growth and on the expression of EcR and Vtg differ depending on the exposure salinity. The solubility of fipronil is demonstrated to decrease considerably at higher salinities. This suggests that fipronil and its photodegradation products may be more bioavailable to benthic organisms as salinity increases, as more chemical would partition to tissues. Our findings suggest that endocrine disruption is occurring through alterations to gene expression in C. sapidus populations exposed to environmental levels of fipronil, and that effects may be dependent upon the salinity at

  20. Mavi Yengecin (Callinectes Sapidus Rathbun 1896) Et Verimi ve Besin Madde İçeriği Üzerine bir Araştırma

    OpenAIRE

    Atar, Hasan H.; Ölmez, Murtaza; Bekcan, Süleyman; Seçer, Selçuk

    2001-01-01

    In this research, it is aimed to determine the efficiency and the nutrient materials of its meat such as moisture, crude protein, crude fat and crude ash of blue crab (Callinectes sapidus). The mean live weigh and meat weight of blue crab was determined as 128.044±10.71 g and 24.365±2.25 g and mean meat yield was23.36±10.71 %. As a result of nutrient materials analysis it was found that the percentages of mean moisture, dry matter, crude protein, crude fat, crude ash, calcium and phosphor of ...

  1. Seasonal Variation and Meat Composition of Blue Crab (Callinectes sapidus, RATHBUN, 1896) Caught in İskenderun Bay, North-East Mediterranean

    OpenAIRE

    TÜRELİ, Canan

    2002-01-01

    The seasonal variation and meat composition of blue crabs (Callinectes sapidus Rathbun, 1896) were investigated. Twenty (10 male, 10 female) crabs were used each season. The total weight of the samples was estimated and the ratios of crude protein, lipid, dry matter and crude ash were obtained from meat of the body and claws. Comparatively higher values of dry matter, crude ash and lipid were determined for the claw meat of males in winter. In the same season the crude protein and lipid co...

  2. The Effects of Season and Sex on the Nutritional Quality of Muscle Types of Blue Crab Callinectes sapidus and Swimming Crab Portunus segnis

    OpenAIRE

    AYAS, Deniz

    2016-01-01

    The effects of season and sex on the nutritional quality of muscle types (lump crab meatLCM, claw crab meat-CCM) of swimming crab (Portunus segnis) and blue crab (Callinectes sapidus) were investigated. Carapace width, carapace length and total weight of both crab species were measured. High protein content in spring and low protein content in autumn were observed for both crab species. The levels of lipid content of both crab species were found to be similar. Higher lipid contents in spri...

  3. Changes in ecdysteroid levels and expression patterns of ecdysteroid-responsive factors and neuropeptide hormones during the embryogenesis of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Techa, Sirinart; Alvarez, Javier V; Sook Chung, J

    2015-04-01

    Embryogenesis requires the involvement and coordination of multiple networks of various genes, according to a timeline governing development. Crustacean embryogenesis usually includes the first molt, a process that is known to be positively controlled by ecdysteroids. We determined the amounts of ecdysteroids, as well as other related factors: the ecdysone receptor (CasEcR), the retinoid X receptor (CasRXR), the molt-inhibiting hormone (CasMIH), and crustacean hyperglycemic hormone (CasCHH) during the ovarian and embryonic developments of Callinectes sapidus. In summary, the ovaries at stages 1-4 have expression levels of maternal CasEcR and CasRXR 10-50 times higher than levels seen in embryos at the yolk stage. This large difference in the amount of the these factors in C. sapidus ovaries suggests that these maternal ecdysteroid-responsive factors may be utilized at the initiation of embryogenesis. During embryogenesis, the changes in total ecdysteroids and levels of CasEcR and CasRXR expression are similar to those observed in juvenile molts. The full-length cDNA sequence of the C. sapidus BTB domain protein (CasBTBDP) initially isolated from Y-organ cDNA, contains only Broad-Complex, Tramtrack, and Bric a brac (BTB) domains. The levels of CasBTBDP are kept constant throughout embryogenesis. The expression profiles of CasMIH and CasCHH are similar to the titers of ecdysteroids. However, the timing of their appearance is followed by increases in CasEcRs and CasRXRs, implying that the expressions of these neuropeptides may be influenced by ecdysteroids. Moreover, the ecdysteroid profile during embryogenesis may track directly with the timing of organogenesis of Y-organs and their activity. Our work reports, for first time, the observed expression and changes of ecdysteroid-responsive factors, along with CasCHH and CasMIH, during embryogenesis in the crustacean C. sapidus. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Abundance and distribution of Portunidae larval phases (Crustacea: Brachyura in the estuarine and coastal region of the Patos Lagoon, southern Brazil

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    Rony Roberto Ramos Vieira

    Full Text Available ABSTRACT: The abundance and distribution of larval phases of the Portunidae found in the estuary of the Patos Lagoon and the coastal region were studied during two years (1995 and 1999. A conical net (165 cm long, 60 cm mouth, and 330 µm mesh equipped with a flowmeter was towed for three minutes at 2 knots at six stations within the estuary and four stations in the coastal region. Samplings were carried out on the surface and near the bottom. At each sampling location, the salinity and temperature were also recorded. In 1995, the zoeae of Arenaeus cribrarius (Lamarck, 1818, Callinectes sapidus Rathbun, 1896 and Achelous spinicarpus Stimpson, 1871 were caught, resulting in a total abundance of 121.98 ind.100 m-3 (90.95 ind.100 m-3 on the surface and 31.03 ind.100 m-3 near the bottom. A total of 452.27 ind.100 m-3 were caught in the megalopa phase (13.49 ind.100 m-3 on the surface and 438.78 ind.100 m-3 near the bottom. In 1999, only zoeae of C. sapidus were caught, resulting in a total abundance of 419.78 ind.100 m-3 (386.98 ind.100 m-3 on the surface and 32.8 ind.100 m-3 near the bottom. Megalopae of these three species were caught, resulting in a total abundance of 179.91 ind.100 m-3 (25.38 ind.100 m-3 on surface and 154.53 ind.100 m-3 near the bottom. Summer was the season with the highest abundance of larvae in both years. During spring and summer, spawning was observed in the estuarine region of the Patos Lagoon.

  5. Comparative Ultrastructure and Carbohydrate Composition of Gastroliths from Astacidae, Cambaridae and Parastacidae Freshwater Crayfish (Crustacea, Decapoda

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    Gérard Alcaraz

    2012-12-01

    Full Text Available Crustaceans have to cyclically replace their rigid exoskeleton in order to grow. Most of them harden this skeleton by a calcification process. Some decapods (land crabs, lobsters and crayfish elaborate calcium storage structures as a reservoir of calcium ions in their stomach wall, as so-called gastroliths. For a better understanding of the cyclic elaboration of these calcium deposits, we studied the ultrastructure of gastroliths from freshwater crayfish by using a combination of microscopic and physical techniques. Because sugars are also molecules putatively involved in the elaboration process of these biomineralizations, we also determined their carbohydrate composition. This study was performed in a comparative perspective on crayfish species belonging to the infra-order Astacidea (Decapoda, Malacostraca: three species from the Astacoidea superfamily and one species from the Parastacoidea superfamily. We observed that all the gastroliths exhibit a similar dense network of protein-chitin fibers, from macro- to nanoscale, within which calcium is precipitated as amorphous calcium carbonate. Nevertheless, they are not very similar at the molecular level, notably as regards their carbohydrate composition. Besides glucosamine, the basic carbohydrate component of chitin, we evidenced the presence of other sugars, some of which are species-specific like rhamnose and galacturonic acid whereas xylose and mannose could be linked to proteoglycan components.

  6. Comparative Ultrastructure and Carbohydrate Composition of Gastroliths from Astacidae, Cambaridae and Parastacidae Freshwater Crayfish (Crustacea, Decapoda)

    Science.gov (United States)

    Luquet, Gilles; Fernández, María S.; Badou, Aïcha; Guichard, Nathalie; Roy, Nathalie Le; Corneillat, Marion; Alcaraz, Gérard; Arias, José L.

    2012-01-01

    Crustaceans have to cyclically replace their rigid exoskeleton in order to grow.Most of them harden this skeleton by a calcification process. Some decapods (land crabs, lobsters and crayfish) elaborate calcium storage structures as a reservoir of calcium ions in their stomach wall, as so-called gastroliths. For a better understanding of the cyclic elaboration of these calcium deposits, we studied the ultrastructure of gastroliths from freshwater crayfish by using a combination of microscopic and physical techniques. Because sugars are also molecules putatively involved in the elaboration process of these biomineralizations, we also determined their carbohydrate composition. This study was performed in a comparative perspective on crayfish species belonging to the infra-order Astacidea (Decapoda, Malacostraca): three species from the Astacoidea superfamily and one species from the Parastacoidea superfamily. We observed that all the gastroliths exhibit a similar dense network of protein-chitin fibers, from macro- to nanoscale, within which calcium is precipitated as amorphous calcium carbonate. Nevertheless, they are not very similar at the molecular level, notably as regards their carbohydrate composition. Besides glucosamine, the basic carbohydrate component of chitin, we evidenced the presence of other sugars, some of which are species-specific like rhamnose and galacturonic acid whereas xylose and mannose could be linked to proteoglycan components. PMID:24970155

  7. Sex determination in crayfish: are intersex Cherax quadricarinatus (Decapoda, Parastacidae) genetically females?

    Science.gov (United States)

    Parnes, S; Khalaila, I; Hulata, G; Sagi, A

    2003-10-01

    In the Australian red-claw crayfish Cherax quadricarinatus (von Martens) (Decapoda, Parastacidae), a gonochoristic species, seven different combinations of intersex individuals (with both male and female genital openings) have been described. However, to date, the genetic basis for this phenomenon has not been investigated. This study was designed to test a simple chromosome-based sex-determination model for C. quadricarinatus that assumes the male to be the homogametic (ZZ) sex. According to our model, intersex individuals that are functionally males are genetically females (WZ). Individual crosses were performed between intersex and female crayfish, with control crosses being performed between normal males and females. The control crosses yielded, in most cases, the expected 1:1 sex ratio in the F1 progeny. Crosses between intersex individuals and females yielded a 1:3 (male:female) sex ratio in most crosses. According to our hypothesis, one-third of the females produced in a cross of a female with an intersex animal should be WW females. The hypothesis was tested by crossing normal males with F1 females, which were progeny of intersex fathers. These crosses yielded almost 100% females, a finding that conforms to the above-suggested sex determination model for C. quadricarinatus and the female WZ genotype of intersex individuals.

  8. Effects of copper on the physiological responses of the commercial crab Lithodes santolla (Decapoda: Anomura larvae

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    Oscar Antonio Amin

    2010-06-01

    Full Text Available Effects of copper toxicity on zoea I of Lithodes santolla (Decapoda: Anomura were analysed. The 96-h LC50 was estimated, resulting in 298.5 µg L-1. Groups of larvae were exposed to sublethal concentrations (40, 80 and 160 µg L-1 for 96 h. Oxygen consumption, ammonia excretion, O:N atomic ratio, lipid peroxidation (LPO and body water content were measured. Oxygen consumption of treated groups (mean 46.92 ± 8.03 µg-atom O2 h-1 mg-1 did not differ significantly with control. Ammonia excretion decreased by 60% at higher Cu concentration (1.61 ± 0.65 µg-atom N-NH3 h-1 mg-1, leading to a 117% increase in the O:N ratio. LPO values during the exposure time were higher in all treatments than in the controls. The water content was significantly higher in treatments than in controls. The highest concentration assayed, which represents about 50% of 96-h LC50, had evident effects on the parameters analysed. The values of copper in water reported for the coastal zone of Ushuaia bay exceed the value established by the United States Environmental Protection Agency (USEPA for ambient water quality criteria. Therefore, the results obtained in the present study are a contribution to the study of potential effects of copper as a common stressor in the first larval stage of this commercial species of the Beagle Channel.

  9. El cangrejo Heterocrypta tommasii (Decapoda: Parthenopidae: adición a la carcinofauna venezolana

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    Juan Bolaños

    2006-12-01

    Full Text Available Se registra por primera vez en aguas marinas venezolanas la presencia del cangrejo Heterocrypta tommasii y se presentan las principales características del espécimen estudiado. Este hallazgo amplía la distribución conocida de la especie (la cual sólo contemplaba Colombia, Guayana y Brasil, establece el primer registro del género Heterocrypta en Venezuela e incrementa a seis el número de especies conocidas de Parthenopidae para este país.The crab Heterocrypta tommasii (Decapoda: Parthenopidae: an addition to the carcinofauna of Venezuela. The crab Heterocrypta tommasii Rodrigues da Costa, 1959 is reported for the first time from Venezuelan marine waters and its main taxonomical features are presented and illustrated. This report extends the range from Colombia, Guyana and Brazil; is the first report of the genus for Venezuela, and increases to six the number of Parthenopidae species known from the country. Rev. Biol. Trop. 54 (Suppl. 3: 131-134. Epub 2007 Jan. 15.

  10. The Larval Development of Penaeus semisulcatus (de Hann, 1850 (Decapoda: Penaeidae.

    Directory of Open Access Journals (Sweden)

    Gürel Türkmen

    2015-12-01

    Full Text Available Penaeus semisulcatus (de Hann, 1850 (Decapoda: Penaeidae’un larval gelişimi. Penaeus semisulcatus’un yapay üretimi üzerine olan çalışma Haziran, 2001 tarihinde Çeşme-İzmir’de bulunan Pınar Deniz Ürünleri (Balık Çiftliği’nde gerçekleştirilmiştir. Çalışmada yumurta almak amacıyla kullanılan olgun dişi bireyler Antalya’dan getirilmiştir. Elde edilen yumurtalar laboratuar koşullarında post larval aşamaya kadar yetiştirilmiştir. Laboratuarda yetiştirilen bireylerin larval aşamaları tanımlanmış ve fotoğraflanmıştır. Larval dönemlerde Isochrysis sp. ve Artemia nauplii yem olarak kullanılmıştır. Altı naupliar, üç protozoeal, üç mysis ve ilk postlarval dönem ana hatlarıyla tanımlanmıştır. P. semisulcatus’un larval morfolojisi diğer Penaeus türleri ve bu tür ile ilgili önceki çalışmalarla karşılaştırılmıştır

  11. Cloning of an insulin-like androgenic gland factor (IAG) from the blue crab, Callinectes sapidus: implications for eyestalk regulation of IAG expression.

    Science.gov (United States)

    Chung, J Sook; Manor, R; Sagi, A

    2011-08-01

    In malacostracan crustaceans, sex differentiation is uniquely regulated by a hormone secreted by the male-specific androgenic gland (AG). An isopod AG hormone was the first to be structurally elucidated and was found to belong to the insulin superfamily of proteins. Recently, it has been found that the AGs of several decapod crustaceans express insulin-like androgenic gland factors (IAGs), whose function is believed to be similar to that of the isopod AG hormone. Here we report the isolation from the blue crab Callinectes sapidus of the full-length cDNA encoding a candidate insulin-like AG hormone, termed Cas-IAG. The predicted protein Cas-IAG was encoded as a precursor consisting of a signal peptide, the B chain, the C peptide, and the A chain in that order. While the AG was the main source of Cas-IAG expression, as found in other decapod species, the hepatopancreas of male Callinectes sapidus crabs displayed minor Cas-IAG expression. Eyestalk ablation confirmed the presence of a possible endocrine axis between the eyestalk ganglia and the AG, implying that Cas-IAG expression is negatively regulated by (a) substance(s) present in the eyestalk ganglia. Copyright © 2011 Elsevier Inc. All rights reserved.

  12. Ecdysteroids regulate the levels of Molt-Inhibiting Hormone (MIH expression in the blue crab, Callinectes sapidus.

    Directory of Open Access Journals (Sweden)

    Sirinart Techa

    Full Text Available Arthropod molt is coordinated through the interplay between ecdysteroids and neuropeptide hormones. In crustaceans, changes in the activity of Y-organs during the molt cycle have been regulated by molt-inhibiting hormone (MIH and crustacean hyperglycemic hormone (CHH. Little has been known of the mode of direct effects of ecdysteroids on the levels of MIH and CHH in the eyestalk ganglia during the molt cycle. This study focused on a putative feedback of ecdysteroids on the expression levels of MIH transcripts using in vitro incubation study with ecdysteroids and in vivo RNAi in the blue crab, Callinectes sapidus. Our results show a specific expression of ecdysone receptor (EcR in which EcR1 is the major isoform in eyestalk ganglia. The initial elevation of MIH expression at the early premolt stages is replicated by in vitro incubations of eyestalk ganglia with ecdysteroids that mimic the intrinsic conditions of D0 stage: the concentration (75 ng/ml and composition (ponasterone A and 20-hydroxyecdysone at a 3:1 (w:w ratio. Additionally, multiple injections of EcR1-dsRNA reduce MIH expression by 67%, compared to the controls. Our data provide evidence on a putative feedback mechanism of hormonal regulation during molting cycle, specifically how the molt cycle is repeated during the life cycle of crustaceans. The elevated concentrations of ecdysteroids at early premolt stage may act positively on the levels of MIH expression in the eyestalk ganglia. Subsequently, the increased MIH titers in the hemolymph at postmolt would inhibit the synthesis and release of ecdysteroids by Y-organs, resulting in re-setting the subsequent molt cycle.

  13. Overwintering of the parasitic dinoflagellate Hematodinium perezi in dredged blue crabs (Callinectes sapidus) from Wachapreague Creek, Virginia.

    Science.gov (United States)

    Shields, Jeffrey D; Sullivan, Shelley E; Small, Hamish J

    2015-09-01

    Parasitic dinoflagellates in the genus Hematodinium cause disease and mortality in several commercially important marine decapod crustaceans. One species, Hematodinium perezi, occurs in blue crabs, Callinectes sapidus, along the eastern seaboard and Gulf coast of the USA. The parasite infects blue crabs, other decapods, and amphipods in the high salinity waters of coastal bays. Epizootics of the parasite often reach prevalence levels of 75-80% during outbreaks with diseased crabs dying from the infection. Prevalence of the parasite is bimodal, with a minor peak in late spring or summer, and a major peak in fall, and declining rapidly to nearly zero in late November and December. The rapid decline in infections in the late fall brings up the question of whether the parasite overwinters in crabs or whether it uses an unidentified resting stage, such as a cyst. We report observations on the prevalence of the parasite from winter dredge surveys undertaken in 2011 and 2012. Crabs were examined via hemolymph smears, histology, and PCR diagnosis for the presence of H. perezi and other pathogens. Active infections were observed from January through March in 2011 and 2012, indicating the parasite can overwinter in blue crabs. However, several crabs that were positive by PCR had presumptive effete infections that were difficult to diagnose in histological slides and hemolymph smears. These infections did not appear to be active and may have been in subsidence. Dredged crabs with light and moderate active infections were held at 15°C to determine if the parasite was capable of rapid progression. In 8 cases, infections exhibited logarithmic growth progressing rapidly over 8-12days. We present evidence that overwintering of H. perezi occurs in the blue crab hosts, that infections are capable of responding rapidly to increases in temperatures, and that overwintering provides a reservoir of infected animals for transmission to occur in the spring. Copyright © 2015 Elsevier Inc

  14. Changes in intracellular calcium concentration in crustacean (Callinectes sapidus) Y-organs: relation to the hemolymphatic ecdysteroid titer.

    Science.gov (United States)

    Chen, Hsiang-Yin; Watson, R Douglas

    2011-01-01

    Secretion of ecdysteroid molting hormones by crustacean Y-organs is negatively regulated (inhibited) by molt-inhibiting hormone (MIH), a neuropeptide produced by neurosecretory cells in eyestalk ganglia. The inhibitory effect of MIH is mediated by one or more cyclic nucleotide second messengers. In addition, available data indicate that ecdysteroidogenesis is positively regulated (stimulated) by intracellular calcium. However, despite the apparent critical role of calcium in regulating ecdysteroidogenesis, the level of Ca(2+) in Y-organs cells has not been previously determined. In studies reported here, eyestalks were ablated from blue crabs (Callinectes sapidus) to remove the endogenous source of MIH and activate Y-organs. At 0, 3, 6, and 9 days after eyestalk ablation (D0, D3, D6, and D9, respectively), the level of Ca(2+) in Y-organ cells was determined using a fluorescent calcium indicator (Fluo-4), and the hemolymphatic ecdysteroid titer was determined by radioimmunoassay. Calcium fluorescence in D6 Y-organs was 3.5-fold higher than that in D0 controls; calcium fluorescence in D9 Y-organs was 3.9-fold higher than in D0 controls (P<0.05). Measurement of fluorescence along a transect drawn through representative cells indicated that the calcium fluorescence was localized to cytoplasm and not to nuclei. Associated with the increase in intracellular Ca(2+) was a significant increase in the hemolymphatic ecdysteroid titer: The level of ecdysteroids in hemolymph rose from 5.5 ng/mL on D0 to 49.6 ng/mL on D6 and 87.2 ng/mL on D9 (P<0.05). The results are consistent with the hypothesis that ecdysteroidogenesis is stimulated by an increase in intracellular Ca(2+).

  15. Ecdysteroids regulate the levels of Molt-Inhibiting Hormone (MIH) expression in the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Techa, Sirinart; Chung, J Sook

    2015-01-01

    Arthropod molt is coordinated through the interplay between ecdysteroids and neuropeptide hormones. In crustaceans, changes in the activity of Y-organs during the molt cycle have been regulated by molt-inhibiting hormone (MIH) and crustacean hyperglycemic hormone (CHH). Little has been known of the mode of direct effects of ecdysteroids on the levels of MIH and CHH in the eyestalk ganglia during the molt cycle. This study focused on a putative feedback of ecdysteroids on the expression levels of MIH transcripts using in vitro incubation study with ecdysteroids and in vivo RNAi in the blue crab, Callinectes sapidus. Our results show a specific expression of ecdysone receptor (EcR) in which EcR1 is the major isoform in eyestalk ganglia. The initial elevation of MIH expression at the early premolt stages is replicated by in vitro incubations of eyestalk ganglia with ecdysteroids that mimic the intrinsic conditions of D0 stage: the concentration (75 ng/ml) and composition (ponasterone A and 20-hydroxyecdysone at a 3:1 (w:w) ratio). Additionally, multiple injections of EcR1-dsRNA reduce MIH expression by 67%, compared to the controls. Our data provide evidence on a putative feedback mechanism of hormonal regulation during molting cycle, specifically how the molt cycle is repeated during the life cycle of crustaceans. The elevated concentrations of ecdysteroids at early premolt stage may act positively on the levels of MIH expression in the eyestalk ganglia. Subsequently, the increased MIH titers in the hemolymph at postmolt would inhibit the synthesis and release of ecdysteroids by Y-organs, resulting in re-setting the subsequent molt cycle.

  16. BIOCHEMICAL AND ULTRASTRUCTURAL ASPECTS OF CA2+ TRANSPORT BY MITOCHONDRIA OF THE HEPATOPANCREAS OF THE BLUE CRAB CALLINECTES SAPIDUS

    Science.gov (United States)

    Chen, Chung-Ho; Greenawalt, John W.; Lehninger, Albert L.

    1974-01-01

    Mitochondria isolated from the hepatopancreas of the blue crab Callinectes sapidus show up to 12-fold stimulation of respiration on addition of Ca2+, which is accompanied by Ca2+ accumulation (Ca2+:site = 1.9) and H+ ejection (H+:Ca2+ = 0.85). Sr2+ and Mn2+ are also accumulated; Mg2+ is not. A strongly hypertonic medium (383 mosM), Mg2+, and phosphate are required for maximal Ca2+ uptake. Ca2+ uptake takes precedence over oxidative phosphorylation of ADP for respiratory energy. Once Ca2+ is accumulated by the crab mitochondria, it is stable and only very slowly released, even by uncoupling agents. ATP hydrolysis also supports Ca2+ uptake. Respiration-inhibited crab hepatopancreas mitochondria show both high-affinity and low-affinity Ca2+-binding sites, which are inactive in the presence of uncoupling agents. Crab hepatopancreas mitochondria have an enormous capacity for accumulation of Ca2+, up to 5,500 ng-atoms Ca2+ per mg protein, with an equivalent amount of phosphate. Freshly isolated mitochondria contain very large amounts of Ca2+, Mg2+, phosphate, K+, and Na+; their high Ca2+ content is a reflection of the vary large amount of extra-mitochondrial Ca2+ in the whole tissue. Electron microscopy of crab mitochondria loaded with Ca2+ and phosphate showed large electron-dense deposits, presumably of precipitated calcium phosphate. They consisted of bundles of needle-like crystals, whereas Ca2+-loaded rat liver mitochondria show only amorphous deposits of calcium phosphate under similar conditions. The very pronounced capacity of crab hepatopancreas mitochondria for transport of Ca2+ appears to be adapted to a role in the storage and release of Ca2+ during the molting cycle of this crustacean. PMID:4827906

  17. The Effects of Acute Copper and Ammonia Challenges on Ammonia and Urea Excretion by the Blue Crab Callinectes sapidus.

    Science.gov (United States)

    Zimmer, Alex M; Jorge, Marianna Basso; Wood, Chris M; Martins, Camila M G; Bianchini, Adalto

    2017-04-01

    Copper (Cu) is a persistent environmental contaminant that elicits several physiological disturbances in aquatic organisms, including a disruption in ammonia regulation. We hypothesized that exposure to Cu in a model crustacean (blue crab, Callinectes sapidus) acclimated to brackish water (2 ppt) would lead to hyperammonemia by stimulating an increase in ammonia production and/or by inhibiting ammonia excretion. We further hypothesized that urea production would represent an ammonia detoxification strategy in response to Cu. In a pilot experiment, exposure to 0, 100, and 200 µg/L Cu for 6 h caused significant concentration-dependent increases in ammonia excretion (J amm ). Based on these results, an acute 24-h 100 µg/L Cu exposure was conducted and this similarly caused an overall stimulation of J amm during the 24-h period, indicative of an increase in ammonia production. Terminal haemolymph total ammonia content (T amm ) was unchanged, suggesting that while ammonia production was increased, there was no inhibition of the excretion mechanism. In support of our second hypothesis, urea excretion (J urea ) increased in response to Cu exposure; haemolymph [urea] was unaffected. This suggested that urea production also was increased. To further test the hypothesis that J urea increased to prevent hyperammonemia during Cu exposure, crabs were exposed to high environmental ammonia (HEA; 2.5 mmol/L NH 4 HCO 3 ) for 12 h in a separate experiment. This led to a fourfold increase in haemolymph T amm , whereas J urea increased only transiently and haemolymph [urea] was unchanged, indicating that urea production likely does not contribute to the attenuation of hyperammonemia in blue crabs. Overall, Cu exposure in blue crabs led to increased ammonia and urea production, which were both eliminated by excretion. These results may have important implications in aquaculture systems where crabs may be exposed to elevated Cu and/or ammonia.

  18. The larval development of the partner shrimp Periclimenes sagittifer (Norman, 1861) (Decapoda: Caridea: Palaemonidae: Pontoniinae) described from laboratory-reared material, with a note on chemical settlement cues

    Science.gov (United States)

    dos Santos, Antonina; Calado, Ricardo; Bartilotti, Cátia; Narciso, Luís

    2004-04-01

    The complete larval development (eight zoeae and megalopa) of Periclimenes sagittifer (Norman, 1861) (Decapoda: Palaemonidae: Pontoniinae) from laboratory-reared material is described and illustrated. The morphology of the first larval stage is compared with previous larval descriptions of other species in the genus (P. agag, P. americanus, P. calmani, P. diversipes, P. grandis and P. pandionis). The importance of chemical settlement cues for late stage Periclimenes larvae is discussed.

  19. Impact of molt-disrupting BDE-47 on epidermal ecdysteroid signaling in the blue crab, Callinectes sapidus, in vitro.

    Science.gov (United States)

    Booth, Ashley; Zou, Enmin

    2016-08-01

    Polybrominated diphenyl ethers (PBDEs) are environmentally pervasive flame retardants that have been linked with endocrine disruption in a variety of organisms. BDE-47, one of the most prevalent congeners found in aquatic environments, has recently been shown to inhibit crustacean molting, but little is known about the specific mechanism through which molt-inhibition occurs. This study examined whether the inhibitory effect on molting arises from the disruption of hormone signaling in the epidermis using the blue crab, Callinectes sapidus, as the model crustacean. First, we partially sequenced cDNA of N-acetyl-β-glucosaminidase (NAG) from the epidermis, a terminal enzyme in the molting hormone-signaling cascades that is commonly used as the biomarker for ecdysteroid signaling. This partial cDNA sequence was then used to create primers for quantification of NAG gene expression. Then, a new tissue culture technique was developed and dubbed the epidermis-with-exoskeleton (EWE) method, wherein epidermal tissue, along with the overlying exoskeleton, is immersed in a medium of physiologically relevant osmolarity. Using this EWE tissue culture method, we assessed the inducibility of NAG mRNA by 20-hydroxyecdysone (20-HE) in vitro. Exposures to 1μM 20-HE were found to induce NAG mRNA at a significantly higher level than the control. Using NAG expression as a biomarker for ecdysteroid signaling, the effects of BDE-47 were measured. BDE-47 alone at 100nM and a combination of 1μM BDE-47 and 1μM 20-HE were found to significantly increase NAG mRNA. A trend of increasing NAG gene expression in the binary BDE-47 exposure as compared to 1μM BDE-47 and 1μM 20-HE alone is suggestive of a synergistic effect of these two chemicals on ecdysteroid signaling in the cultured epidermis. Discussion on the mechanism for inhibition of crustacean molting by BDE-47 is presented. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. De novo assembly of a transcriptome from juvenile blue crabs (Callinectes sapidus) following exposure to surrogate Macondo crude oil.

    Science.gov (United States)

    Yednock, Bree K; Sullivan, Timothy J; Neigel, Joseph E

    2015-07-11

    The blue crab, Callinectes sapidus, is economically and ecologically important in western Atlantic and Gulf of Mexico coastal estuaries. In 2010 blue crabs in the northern Gulf of Mexico were exposed to crude oil and chemical dispersants from the Deepwater Horizon oil spill. To characterize the blue crab transcriptome and identify genes that could be regulated in response to oil exposure we sequenced transcriptomes from hepatopancreas and gill tissues of juvenile blue crabs after exposing them to a water-accommodated fraction of surrogate Macondo crude oil in the laboratory and compared them to transcriptomes from an unexposed control group. Illumina sequencing provided 42.5 million paired-end sequencing reads for the control group and 44.9 million paired-end reads for the treatment group. From these, 73,473 transcripts and 52,663 genes were assembled. Comparison of control and treatment transcriptomes revealed about 100 genes from each tissue type that were differentially expressed. However, a much larger number of transcripts, approximately 2000 from each tissue type, were differentially expressed. Several examples of alternatively spliced transcripts were verified by qPCR, some of which showed significantly different expression patterns. The combined transcriptome from all tissues and individuals was annotated to assign putative gene products to both major gene ontology categories as well as specific roles in responses to cold and heat, metabolism of xenobiotic compounds, defence, hypoxia, osmoregulation and ecdysis. Among the annotations for upregulated and alternatively-spliced genes were candidates for the metabolism of oil-derived compounds. Previously, few genomic resources were available for blue crabs or related brachyuran crabs. The transcriptome sequences reported here represent a major new resource for research on the biology of blue crabs. These sequences can be used for studies of differential gene expression or as a source of genetic markers. Genes

  1. Distribuição e ecologia dos decapoda numa área estuarina de Ubatuba (SP

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    Jorge de Abreu

    1980-12-01

    Full Text Available Benthic macro fauna (Decapoda was sampled by beam trawl at 6 stations in Bay of Fortaleza and adjacents Escuro e Claro rivers (São Paulo, Ubatuba, Brasil. Samples were taken monthly during 1973-1974. Diversity was high in muddy sand sites of Bay of Fortaleza and low at very shallow stations located in the rivers, where the effects of extreme fluctuations of salinity were evident. The changes in species composition appeared to correspond most clearly to differences in salinity. The total number of species of decapods collected in the areas was 27 (14 species of Natantia and 13 of Reptantia. Juveniles of Penaeus brasiiiensis and Penaeus paulensis reach the rivers. Callinectes danae live in the Bay and in the rivers. Xiphopeneus kroyeri is a specie who inhabit the Bay of Fortaleza in high populational density.

  2. Gastropod shell species utilized by hermit crabs (Decapoda: Anomura along the Turkish coast of the Levantine Sea

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    Tahir Ozcan

    2013-06-01

    Full Text Available The present study aimed to describe the gastropod shell species utilized by hermit crabs (Decapoda: Anomura collected during investigations of the decapod fauna of the Levantine Sea coast of Turkey. Specimens were collected from July 2005 to October 2005. A total of 715 individuals belonging to 9 hermit crab species occupying 47 gastropod shell species were collected. The hermit crabs, Diogenes pugilator (Roux, 1829 and Cestopagurus timidus (Roux, 1830 had the highest inhabitation frequency (IF, inhabiting 23.92% and 23.78% of all the samples collected respectively. The nine hermit crab species captured utilizing shells fromforty-seven gastropods species. Cerithium scabridum Philippi, 1849 (100% was the most commonly utilized shell by all hermit crabs.

  3. Decapoda: Potamonautidae

    African Journals Online (AJOL)

    1999-02-09

    Feb 9, 1999 ... The classification of aquatic inland invertebrates has received much systematic attention. Despite such efforts ..... The population hiology and genetics of the deep sea spider crab, Encephaloidcs armstrongi Wood-Mason 1891 ... Tilt: biology of Crustacea, VuJumc II Em- hryology, Morphulol!Y i.md Genetic:>.

  4. Elucidation of the regio- and chemoselectivity of enzymatic allylic oxidations with Pleurotus sapidus – conversion of selected spirocyclic terpenoids and computational analysis

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    Verena Weidmann

    2013-10-01

    Full Text Available Allylic oxidations of olefins to enones allow the efficient synthesis of value-added products from simple olefinic precursors like terpenes or terpenoids. Biocatalytic variants have a large potential for industrial applications, particularly in the pharmaceutical and food industry. Herein we report efficient biocatalytic allylic oxidations of spirocyclic terpenoids by a lyophilisate of the edible fungus Pleurotus sapidus. This ‘’mushroom catalysis’’ is operationally simple and allows the conversion of various unsaturated spirocyclic terpenoids. A number of new spirocyclic enones have thus been obtained with good regio- and chemoselectivity and chiral separation protocols for enantiomeric mixtures have been developed. The oxidations follow a radical mechanism and the regioselectivity of the reaction is mainly determined by bond-dissociation energies of the available allylic CH-bonds and steric accessibility of the oxidation site.

  5. Effects of salinity on the accumulation of hemocyte aggregates and bacteria in the gills of Callinectes sapidus, the Atlantic blue crab, injected with Vibrio campbellii.

    Science.gov (United States)

    Ikerd, Jennifer L; Burnett, Karen G; Burnett, Louis E

    2015-05-01

    In addition to respiration and ion regulation, crustacean gills accumulate and eliminate injected particles, along with hemocyte aggregates that form in response to those particles. Here we report that the dose of Vibrio campbellii previously shown to induce a decrease in respiration and hemolymph flow across the gill in the Atlantic blue crab, Callinectes sapidus, also triggered the formation of aggregates containing four or more hemocytes in the gills, compared with saline-injected controls. More bacteria were trapped and rendered non-culturable per unit weight by anterior respiratory gills than posterior gills specialized for ion regulation. Further, more bacteria accumulated in the anterior gills of animals held at 30 ppt than those at 10 ppt. Thus, the role of the gills in immune defense comes at an energetic cost to this and likely to other crustaceans; this cost is influenced by acclimation salinity and the position and specialized function of individual gills. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. The Effects of Season and Sex on the Nutritional Quality of Muscle Types of Blue Crab Callinectes sapidus and Swimming Crab Portunus segnis

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    Deniz Ayas

    2016-05-01

    Full Text Available The effects of season and sex on the nutritional quality of muscle types (lump crab meatLCM, claw crab meat-CCM of swimming crab (Portunus segnis and blue crab (Callinectes sapidus were investigated. Carapace width, carapace length and total weight of both crab species were measured. High protein content in spring and low protein content in autumn were observed for both crab species. The levels of lipid content of both crab species were found to be similar. Higher lipid contents in spring and winter, lower lipid contents in summer and autumn for both sexes were found. Although both crab species contain small amounts of fat, they are good sources of n-3 PUFA content (especially EPA and DHA for all seasons regardless of sex and muscle types.

  7. Salinity-induced changes in gene expression from anterior and posterior gills of Callinectes sapidus (Crustacea: Portunidae) with implications for crustacean ecological genomics.

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    Havird, Justin C; Mitchell, Reed T; Henry, Raymond P; Santos, Scott R

    2016-09-01

    Decapods represent one of the most ecologically diverse taxonomic groups within crustaceans, making them ideal to study physiological processes like osmoregulation. However, prior studies have failed to consider the entire transcriptomic response of the gill - the primary organ responsible for ion transport - to changing salinity. Moreover, the molecular genetic differences between non-osmoregulatory and osmoregulatory gill types, as well as the hormonal basis of osmoregulation, remain underexplored. Here, we identified and characterized differentially expressed genes (DEGs) via RNA-Seq in anterior (non-osmoregulatory) and posterior (osmoregulatory) gills during high to low salinity transfer in the blue crab Callinectes sapidus, a well-studied model for crustacean osmoregulation. Overall, we confirmed previous expression patterns for individual ion transport genes and identified novel ones with salinity-mediated expression. Notable, novel DEGs among salinities and gill types for C. sapidus included anterior gills having higher expression of structural genes such as actin and cuticle proteins while posterior gills exhibit elevated expression of ion transport and energy-related genes, with the latter likely linked to ion transport. Potential targets among recovered DEGs for hormonal regulation of ion transport between salinities and gill types included neuropeptide Y and a KCTD16-like protein. Using publically available sequence data, constituents for a "core" gill transcriptome among decapods are presented, comprising genes involved in ion transport and energy conversion and consistent with salinity transfer experiments. Lastly, rarefication analyses lead us to recommend a modest number of sequence reads (~10-15M), but with increased biological replication, be utilized in future DEG analyses of crustaceans. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Molt-inhibiting hormone stimulates vitellogenesis at advanced ovarian developmental stages in the female blue crab, Callinectes sapidus 1: an ovarian stage dependent involvement.

    Science.gov (United States)

    Zmora, Nilli; Trant, John; Zohar, Yonathan; Chung, J Sook

    2009-07-07

    To understand the hormonal coordination of the antagonism between molting and reproduction in crustaceans, the terminally anecdysial mature female Callinectes sapidus was used as a model. The regulatory roles of crustacean hyperglycemic hormone (CHH) and molt-inhibiting hormone (MIH) in vitellogenesis were examined. A competitive specific RIA was used to measure the levels of MIH and CHH in the hemolymphs of mature females at pre- and mid- vitellogenic stages, and their effects on vitellogenesis at early (early 2, E2) and mid vitellogenesis (3) stages were determined in vitro. A hepatopancreas fragments incubation system was developed and the levels of vitellogenin (VtG), as well as VtG mRNA and heterogeneous nuclear (hn)VtG RNA were determined using RIA or QPCR, respectively. MIH titers were four times higher at mid-vitellogenesis than at pre-vitellogenesis, while CHH levels in the hemolymph were constant. In the in vitro incubation experiments, MIH increased both VtG mRNA levels and secretion at ovarian stage 3. At stage E2, however, MIH resulted in a mixed response: downregulation of VtG mRNA and upregulation of hnVtG RNA. CHH had no effect on any of the parameters. Actinomycin D blocked the stimulatory effects of MIH in stage 3 animals on VtG mRNA and VtG, while cycloheximide attenuated only VtG levels, confirming the MIH stimulatory effect at this stage. MIH is a key endocrine regulator in the coordination of molting and reproduction in the mature female C. sapidus, which simultaneously inhibits molt and stimulates vitellogenesis.

  9. Seasonal variations in reproductive activity of the blue crab, Callinectes sapidus: Vitellogenin expression and levels of vitellogenin in the hemolymph during ovarian development.

    Science.gov (United States)

    Thongda, Willawan; Chung, J Sook; Tsutsui, Naoaki; Zmora, Nilli; Katenta, Anna

    2015-01-01

    In general, season affects the physiology and behavior of most animals. Warmer temperatures accelerate growth and reproduction of ectotherms, whereas these processes are slowed or halted in colder temperatures. Female blue crabs, Callinectes sapidus inhabiting the Chesapeake Bay, exhibit a seasonal migratory behavior that is closely tied with spawning and the release of larvae. To better understand reproductive activities of the migratory adult females, we examined two reproductive parameters of these crabs sampled monthly (April-December, 2006): the levels of vitellogenin (VtG) in the hemolymph and VtG expression in the hepatopancreas and ovary. The full-length cDNA of VtG (CasVtG-ova) has been isolated from the ovary. The putative CasVtG sequence found in the ovary is >99% identical to that of the hepatopancreas and is related most closely to the sequences reported in other crab species. In female C. sapidus, the hepatopancreas produces over 99% of the total VtG toward the ovarian development. Ovarian stages 2 and 3 in the sampled females are characterized by significant high levels of VtG in hemolymph and VtG expression in both the hepatopancreas and ovary. However, during the southbound migration in fall, females at ovarian stages 2 and 3 have decreased VtG levels, compared to those in spring and summer. The decreased vitellogenesis activity during the fall migration suggests seasonal adaptation to ensure successful spawning and the larval release. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. mRNA Expression and activity of ion-transporting proteins in gills of the blue crab Callinectes sapidus: effects of waterborne copper.

    Science.gov (United States)

    Martins, Camila M G; Almeida, Daniela Volcan; Marins, Luis Fernando Fernandes; Bianchini, Adalto

    2011-01-01

    Waterborne Cu effects on the transcription of genes encoding ion-transporting proteins and the activities of these proteins were evaluated in gills of the blue crab Callinectes sapidus acclimated to diluted (2‰) and full (30‰) seawater. Crabs were exposed (96 h) to an environmentally relevant concentration of dissolved Cu (0.78 µM) and had their posterior (osmoregulating) gills dissected for enzymatic and molecular analysis. Endpoints analyzed were the activity of key enzymes involved in crab osmoregulation (sodium-potassium adenosine triphosphatase [Na(+)/K(+)-ATPase], hydrogen adenosine triphosphatase [H(+)-ATPase], and carbonic anhydrase [CA]) and the mRNA expression of genes encoding these enzymes and the sodium-potassium-chloride (Na(+)/K(+)/2Cl⁻) cotransporter. Copper effects were observed only in crabs acclimated to diluted seawater (hyperosmoregulating crabs) and were associated with an inhibition of the expression of mRNA of genes encoding the Na(+)/K(+)-ATPase and the Na(+)/K(+)/2Cl⁻ cotransporter. However, Cu did not affect Na(+)/K(+)-ATPase activity, indicating that the gene transcription is downregulated before a significant inhibition of the enzyme activity can be observed. This also suggests the existence of a compensatory response of this enzyme to prevent osmoregulatory disturbances after short-term exposure to environmentally relevant Cu concentrations. These findings suggest that Cu is a potential ionoregulatory toxicant in blue crabs C. sapidus acclimated to low salinity. The lack of Cu effect on blue crabs acclimated to full seawater would be due to the reduced ion uptake needed for the regulation of the hemolymph osmotic concentration in full seawater (30‰). Also, this could be explained considering the lower bioavailability of toxic Cu (free ion) associated with the higher ionic content and dissolved organic matter concentration in high salinity (30‰) than in diluted seawater (2‰). © 2010 SETAC.

  11. Metal Accumulations in Water, Sediment, Crab (Callinectes sapidus) and Two Fish Species (Mugil cephalus and Anguilla anguilla) from the Köyceğiz Lagoon System-Turkey: An Index Analysis Approach.

    Science.gov (United States)

    Genç, Tuncer Okan; Yilmaz, Fevzi

    2017-08-01

    The concentrations of six metals (Hg, Cd, Cu, Pb, Cr and Zn) individual total metal load (IMBI) values and its relation to condition index were determined in water, sediment and tissues of crab (Callinectes sapidus) and two fish species (Mugil cephalus and Anguilla anguilla) inhabiting Köyceğiz Lagoon System. The average distribution of the IMBI values ranged from 0.033 to 0.265. Distribution patterns of IMBI in species follow the sequence: A. anguilla > M. cephalus > C. sapidus. Results showed that there are positive relationships between species sizes and metal levels in most cases. The concentrations of Pb in muscle in the three studied species were in all cases considerably higher than the maximum levels set by law. Average Cd, Cu and Zn values in M. cephalus were also higher than the limits proposed for fish by FAO/WHO, EC and TFC. Therefore, the human consumption of all analysed species is not recommended.

  12. (1)H NMR metabolomic profiling of the blue crab (Callinectes sapidus) from the Adriatic Sea (SE Italy): A comparison with warty crab (Eriphia verrucosa), and edible crab (Cancer pagurus).

    Science.gov (United States)

    Zotti, Maurizio; De Pascali, Sandra Angelica; Del Coco, Laura; Migoni, Danilo; Carrozzo, Leonardo; Mancinelli, Giorgio; Fanizzi, Francesco Paolo

    2016-04-01

    The metabolomic profile of blue crab (Callinectes sapidus) captured in the Acquatina lagoon (SE Italy) was compared to an autochthonous (Eriphia verrucosa) and to a commercial crab species (Cancer pagurus). Both lipid and aqueous extracts of raw claw muscle were analyzed by (1)H NMR spectroscopy and MVA (multivariate data analysis). Aqueous extracts were characterized by a higher inter-specific discriminating power compared to lipid fractions. Specifically, higher levels of glutamate, alanine and glycine characterized the aqueous extract of C. sapidus, while homarine, lactate, betaine and taurine characterized E. verrucosa and C. pagurus. On the other hand, only the signals of monounsaturated fatty acids distinguished the lipid profiles of the three crab species. These results support the commercial exploitation and the integration of the blue crab in human diet of European countries as an healthy and valuable seafood. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Dos nuevas especies de cangrejos de agua dulce (Brachyura: Pseudothelphusidae de la Serranía de Los Paraguas, Cordillera Occidental de Colombia

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    Gabriel E Ramos-Tafur

    2006-09-01

    Full Text Available Se describen dos nuevas especies de cangrejos de agua dulce del género Hypolobocera de la Serranía de Los Paraguas, Cordillera Occidental de Colombia. H. solimani n. sp. está estrechamente relacionada con H. alata Campos 1989, pero se puede distinguir de ésta por el margen superior del mero del tercer maxilípedo, el cual es liso; por la forma del ápice del primer gonópodo y la forma del lóbulo interno o papila del canal espermático, la cual es cóncava, levantada y termina bordeada por diminutas papilas o tubérculos redondeados. H. triangula n. sp. es muy similar a H. rotundilobata Rodríguez 1994, pero se diferencia de ésta por el tamaño y la forma del lóbulo lateral, una cresta basal de tubérculos y la superficie apical del gonópodo. Con estas dos nuevas descripciones se incrementa a 20 el número de miembros válidos del género Hypolobocera conocidos del occidente de Colombia o del Chocó biogeográfico.Two new species of fresh water crab (Brachyura: Pseudothelphusidae from Serranía de Los Paraguas, Cordillera Occidental, Colombia. Two new species of freshwater crabs of the genus Hypolobocera from Serranía de Los Paraguas, Cordillera Occidental, Colombia, are described. Hypolobocera solimani n. sp. is closely related to H. alata Campos 1989, but can be distinguished from it by smooth upper margin of third maxilliped merus, the size and shape of apex of first gonopod, and the internal lobe or papilla of spermatic channel, which is concave, raised, with ends with minute papillae or rounded tubercles. Hypolobocera triangula n. sp. is very similar to H. rotundilobata Rodríguez 1994, but differs from it by the shape and size of the lateral lobe, an oblique basal ridge of tubercles and by the apex of first gonopod. With the two new species, the total number of valid members of the genus Hypolobocera known from western Colombia or biogeographic Chocó is 20. Rev. Biol. Trop. 54 (3: 1047-1056. Epub 2006 Sept. 29.

  14. Width-weight relationship and condition factor of Ucides cordatus (Crustacea, Decapoda, Ucididae at tropical mangroves of Northeast Brazil

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    Marina S.L.C. Araújo

    2012-09-01

    Full Text Available The present contribution aims at evaluating the carapace width vs. humid weight relationship and the condition factor of Ucides cordatus (Linnaeus, 1763, in the mangrove forests of the Ariquindá and Mamucabas rivers, state of Pernambuco, Brazil. These two close areas present similar characteristics of vegetation and substrate, but exhibit different degrees of environmental conservation: the Ariquindá River is the preserved area, considered one of the last non-polluted of Pernambuco, while the Mamucabas River suffers impacts from damming, deforestation and deposition of waste. A total of 1,298 individuals of U. cordatus were collected. Males were larger and heavier than females, what is commonly observed in Brachyura. Ucides cordatus showed allometric negative growth (p < 0.05, which is probably related to the dilatation that this species develops in the lateral of the carapace, which stores six pairs of gills. The values of b were within the limit established for aquatic organisms. Despite of the condition factor being considered an important feature to confirm the reproductive period, since it varies with cyclic activities, in the present study it was not correlated to the abundance of ovigerous females. However, it was considered a good parameter to evaluate environmental impacts, being significantly lower at the impacted area.

  15. The complete mitochondrial genome of the stomatopod crustacean Squilla mantis

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    Cook Charles E

    2005-08-01

    Full Text Available Abstract Background Animal mitochondrial genomes are physically separate from the much larger nuclear genomes and have proven useful both for phylogenetic studies and for understanding genome evolution. Within the phylum Arthropoda the subphylum Crustacea includes over 50,000 named species with immense variation in body plans and habitats, yet only 23 complete mitochondrial genomes are available from this subphylum. Results I describe here the complete mitochondrial genome of the crustacean Squilla mantis (Crustacea: Malacostraca: Stomatopoda. This 15994-nucleotide genome, the first described from a hoplocarid, contains the standard complement of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a non-coding AT-rich region that is found in most other metazoans. The gene order is identical to that considered ancestral for hexapods and crustaceans. The 70% AT base composition is within the range described for other arthropods. A single unusual feature of the genome is a 230 nucleotide non-coding region between a serine transfer RNA and the nad1 gene, which has no apparent function. I also compare gene order, nucleotide composition, and codon usage of the S. mantis genome and eight other malacostracan crustaceans. A translocation of the histidine transfer RNA gene is shared by three taxa in the order Decapoda, infraorder Brachyura; Callinectes sapidus, Portunus trituberculatus and Pseudocarcinus gigas. This translocation may be diagnostic for the Brachyura. For all nine taxa nucleotide composition is biased towards AT-richness, as expected for arthropods, and is within the range reported for other arthropods. Codon usage is biased, and much of this bias is probably due to the skew in nucleotide composition towards AT-richness. Conclusion The mitochondrial genome of Squilla mantis contains one unusual feature, a 230 base pair non-coding region has so far not been described in any other malacostracan. Comparisons with other

  16. The complete mitochondrial genome of the stomatopod crustacean Squilla mantis.

    Science.gov (United States)

    Cook, Charles E

    2005-08-09

    Animal mitochondrial genomes are physically separate from the much larger nuclear genomes and have proven useful both for phylogenetic studies and for understanding genome evolution. Within the phylum Arthropoda the subphylum Crustacea includes over 50,000 named species with immense variation in body plans and habitats, yet only 23 complete mitochondrial genomes are available from this subphylum. I describe here the complete mitochondrial genome of the crustacean Squilla mantis (Crustacea: Malacostraca: Stomatopoda). This 15994-nucleotide genome, the first described from a hoplocarid, contains the standard complement of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a non-coding AT-rich region that is found in most other metazoans. The gene order is identical to that considered ancestral for hexapods and crustaceans. The 70% AT base composition is within the range described for other arthropods. A single unusual feature of the genome is a 230 nucleotide non-coding region between a serine transfer RNA and the nad1 gene, which has no apparent function. I also compare gene order, nucleotide composition, and codon usage of the S. mantis genome and eight other malacostracan crustaceans. A translocation of the histidine transfer RNA gene is shared by three taxa in the order Decapoda, infraorder Brachyura; Callinectes sapidus, Portunus trituberculatus and Pseudocarcinus gigas. This translocation may be diagnostic for the Brachyura. For all nine taxa nucleotide composition is biased towards AT-richness, as expected for arthropods, and is within the range reported for other arthropods. Codon usage is biased, and much of this bias is probably due to the skew in nucleotide composition towards AT-richness. The mitochondrial genome of Squilla mantis contains one unusual feature, a 230 base pair non-coding region has so far not been described in any other malacostracan. Comparisons with other Malacostraca show that all nine genomes, like most other

  17. Breeding period in the mangrove crab Goniopsis cruentata (Decapoda: Grapsidae in Northeast Brazil

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    José Jonathas Pereira Rodrigues de Lira

    2013-03-01

    Full Text Available The brachyuran crabs are iteroparous species which present a high diversification of reproduction patterns, which may have evolved as a species-specific response to environmental conditions. Tropical species commonly present a year-round reproduction due to stable environment conditions. Goniopsis cruentata is a crab species widely distributed along the Western Atlantic, inhabiting practically every microhabitat in the mangrove ecosystem. The aim of the present study is to determine the breeding period of the crab Goniopsis cruentata in Northeastern Brazil and also to evaluate the influence of water salinity, rainfall and air and water temperature on it. A total of 71 ovigerous females, captured from August-2007 to July-2008, were used to assess the breeding period of this species. It was analyzed by the monthly proportion of ovigerous females. A correlation was applied to verify the influence of the abiotic factors on the breeding period. The present population bred seasonal-continuously with peaks in the dry period, which was not associated with monthly variations of salinity, rainfall and air and water temperatures. Therefore, according to statistical analyses, our hypothesis was refuted. However, breeding was intensified in the dry period, when salinity and temperatures were higher and rainfall was lower. We conclude that, even though breeding is not related to monthly variation of environmental factors, it occurs in periods of higher salinity and temperatures and lower rainfall.Los cangrejos Brachyura son especies iteróparas, que presentan alta diversificación en los patrones de reproducción, que pueden haber evolucionado como una respuesta específica de la especie a las condiciones ambientales. Las especies tropicales frecuentemente presentan una reproducción en todo el año debido a las condiciones estables del medio. El cangrejo Goniopsis cruentata es una especie ampliamente distribuida en largo del Atlántico occidental, y que

  18. Elevated water temperature increases the levels of reo-like virus and selected innate immunity genes in hemocytes and hepatopancreas of adult female blue crab, Callinectes sapidus.

    Science.gov (United States)

    Chung, J Sook; Pitula, J S; Schott, E; Alvarez, J V; Maurer, L; Lycett, K A

    2015-11-01

    Seasonal changes in water temperature directly affect the aquatic ecosystem. The blue crab, Callinectes sapidus, inhabiting the Chesapeake Bay has been adapted to seasonal changes of the environmental conditions. In this, the animals halt their physiological process of the growth and reproduction during colder months while they resume these processes as water temperatures increase. We aimed to understand the effect of the elevated temperatures on a disease progression of reo-like virus (CsRLV) and innate immunity of adult female C. sapidus. Following a rise in water temperature from 10 to 23 °C, CsRLV levels in infected crabs rose significantly in hemocytes and multiple organs. However, in hemocytes, the elevated temperature had no effect on the levels of three innate immune genes: Cas-ecCuZnSOD-2, CasPPO and CasLpR three carbohydrate metabolic genes: CasTPS, CasGlyP; and CasTreh and the total hemocyte counts (THC). Interestingly, the hemocytes of CsRLV infected animals exposed to 23 °C for 10 days had significantly elevated levels of Cas-ecCuZnSOD-2 and CasTPS, compared to those of the uninfected ones also exposed to the same condition and compared to hatchery-raised females kept at 23 °C. Despite the lack of changes in THC, the types of hemocytes from the animals with high CsRLV levels differed from those of uninfected ones and from hatchery animals kept at 23 °C: CsRLV-infected crabs had hemocytes of smaller size with less cytosolic complexity than uninfected crabs. It therefore appears that the change in temperature influences rapid replication of CsRLV in all internal tissues examined. This implies that CsRLV may have broad tissue tropism. Interestingly, the digestive tract (mid- and hindgut) contains significantly higher levels of CsRLV than hemocytes while hepatopancreas and ovary have lower levels than hemocytes. Innate immune responses differ by tissue: midgut and hepatopancreas with upregulated Cas-ecCuZnSOD-2 similar to that found in hemocytes. By

  19. Ecdysone and retinoid-X receptors of the blue crab, Callinectes sapidus: cloning and their expression patterns in eyestalks and Y-organs during the molt cycle.

    Science.gov (United States)

    Techa, Sirinart; Chung, J Sook

    2013-09-15

    Crustacean molting is known to be regulated largely by ecdysteroids and crustacean hyperglycemic hormone (CHH) neuropeptide family including molt-inhibiting hormone (MIH) and CHH. The surge of 20-OH ecdysone and/or ponasterone A initiates the molting process through binding to its conserved heterodimeric nuclear receptor: Ecdysone Receptor (EcR) and Ultraspiracle (USP)/Retinoid-X Receptor (RXR). To better understand the role of ecdysteroids in the molt regulation, the full-length cDNAs of the blue crab, Callinectes sapidus EcR1 and RXR1 were isolated from the Y-organs and their expression levels were determined in both Y-organs and eyestalks at various molt stages. Y-organs show the expression of four putative isoforms of CasEcRs and CasRXRs which differ in the length of the open reading frame but share the same domain structures as in typical nuclear receptors: AF1, DBD, HR, LBD, and AF2. The putative CasEcR isoforms are derived from a 27-aa insert in the HR and a 49-aa residue substitution in the LBD. In contrast, an insertion of a 5-aa and/or a 45-aa in the DBD and LBD gives rise to CasRXR isoforms. The eyestalks and Y-organs show the co-expression of CasEcRs and CasRXRs but at the different levels. In the eyestalks, the expression levels of CasRXRs are 3-5 times higher than those of CasEcRs, while in Y-organs, CasRXRs are 2.5-4 times higher than CasEcRs. A tissue-specific response to the changes in the levels of hemolymphatic ecdysteroids indicates that these tissues may have differences in the sensitivity or responsiveness to ecdysteroids. The presence of upstream open reading frame and internal ribosome entry site in 5' UTR sequences of C. sapidus and other arthropod EcR/RXR/USP analyzed by in silico indicates a plausible, strong control(s) of the translation of these receptors. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Evaluation of 5.8S rRNA to identify Penaeus semisulcatus and its subspecies, Penaeus semisulcatus persicus (Penaeidae and some Decapoda species

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    Zahra Noroozi

    2015-10-01

    Full Text Available The green tiger prawn, Penaeus semisulcatus is one of the most important members of the family Penaeidae in the Persian Gulf. Based on the morphological characteristics, two groups, including P. semisulcatus and its subspecies viz. P. s. persicus are recognized. This study was conducted to investigate the genetic distance between P. semisulcatus and P. s. persicus by analyzing partial sequence of 5.8S rRNA. Another objective of this study is to evaluate the ability of 5.8S rRNA to identify the species of Decapoda. The results indicated that the 5.8S rRNA gene of both P. semisulcatus and P. s. persicus were exactly identical, and sequence variation was not observed. The results also indicated that 5.8S rRNA sequences between species of the same genus of analysed species of Decapoda are conserved, and no genetic distance was observed in species level. The low evolutionary rate and efficient conservation of the 5.8S rRNA can be attributed to its role in the translation process.

  1. A new microsporidium, Triwangia caridinae gen. nov., sp. nov. parasitizing fresh water shrimp, Caridina formosae (Decapoda: Atyidae) in Taiwan.

    Science.gov (United States)

    Wang, Tai-Chuan; Nai, Yu-Shin; Wang, Chih-Yuan; Solter, Leellen F; Hsu, Hui-Chen; Wang, Chung-Hsiung; Lo, Chu-Fang

    2013-03-01

    A new microsporidium was isolated from the endemic, Taiwanese shrimp, Caridina formosae (Decapoda, Atyidae) from northern Taiwan. A conspicuous symptom of infection was presence of opaque white xenomas located in the proximity of the alimentary tract, the surface of the hepatopancreas, and the gills. A fully developed xenoma consisted of a hard, thick capsule filled with sporophorous vesicles containing multiple spores. Microsporidia developed synchronously within the same sporophorous vesicle, although the stage of parasite development differed among the vesicles. Fresh spores were pyriform, mononucleated and measured 6.53 × 4.38 μm. The polar filament was anisofilar with 9-11 coils. Phylogenetic analysis based on the small subunit ribosomal DNA sequence showed that the isolate is most similar to the fish microsporidian clade containing the genera Kabatana, Microgemma, Potaspora, Spraguea, and Teramicra. The highest sequence identity, 80%, was with Spraguea spp. Based on pathogenesis, life cycle and phylogenetic analysis, we erect a new genus and species, Triwangia caridinae for the novel microsporidium. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. Seamount influences on mid-water shrimps (Decapoda) and gnathophausiids (Lophogastridea) of the South-West Indian Ridge

    Science.gov (United States)

    Letessier, Tom B.; De Grave, Sammy; Boersch-Supan, Philipp H.; Kemp, Kirsty M.; Brierley, Andrew S.; Rogers, Alex D.

    2017-02-01

    Maintenance of often-observed elevated levels of pelagic diversity and biomass on seamounts, of relevance to conservation and fishery management, involves complex interactions between physical and biological variables that remain poorly understood. To untangle these biophysical processes we explore factors influencing the distribution of epi- and meso-pelagic (0-1000 m) micronektonic crustaceans (>15 mm; order Lophogastridea, family Gnathophausiidea; and order Decapoda) on and off seamounts along the South West Indian Ridge (SWIR, 27° to 42°S) and on a seamount off the Madagascar Ridge (31.6°S, 42.8°E). Thirty-one species of micronektic crustaceans were caught using mid-water trawls within the study area but there was no apparent latitude-related patterns in species richness or abundance. Species richness predicted by rarefraction curves and numerical abundance was highest in the vicinity (800 m). The dominant species assemblage comprised the shrimps Systellaspis debilis (37%) and Sergia prehensilis (34%), and was restricted to seamounts on the subtropical SWIR. Our observations suggest that the 'oasis effect' of seamounts conventionally associated with higher trophic levels is also applicable to pelagic micronektic crustaceans at lower trophic levels. We suggest that the enhanced biomass and species richness attributed is due to 'habitat enrichment', whereby seamounts provide favourable habitats for both pelagic and bentho-pelagic mid-water crustaceans.

  3. Ultrastructural aspects of a new species, Vavraia mediterranica (Microsporidia, Pleistophoridae), parasite of the French Mediterranean shrimp, Crangon crangon (Crustacea, Decapoda).

    Science.gov (United States)

    Azevedo, C

    2001-11-01

    The life cycle stages of a new species of the genus Vavraia (Microsporidia, Pleistophoridae), which parasitizes the shrimp Crangon crangon (Crustacea, Decapoda), were examined by light and electron microscopy. This parasite was monomorphic with polysporous sporogony and developed in the skeletal muscle of the host. The multinucleate sporogonial plasmodium divided by plasmotomy and multiple division into uninucleate sporoblasts. All stages were surrounded by a thick and amorphous dense coat external to the plasmalemma. This structure gradually became a merontogenetic sporophorous vacuole (MSV) where the sporonts developed into sporoblasts. The MSV was filled with episporontal granular secretory products and eventually contained up to 50 uninucleate spores. During spore morphogenesis, these episporontal granular products within the MSV became organized as episporontal tubular-like structures. In transverse sections, these structures showed a mean diameter of 1.0 microm, but disappeared during the final phase of the spore maturation. Mature spores were ellipsoidal to slightly pyriform and measured 2.30 x 1.41 microm. The polar filament was anisofilar and consisted of a single coil with six to seven turns (rarely five). This new species is named Vavraia mediterranica n. sp.

  4. Larval Pseudoproleptus sp. (Nematoda: Cystidicolidae) found in the Amazon river prawn Macrobrachium amazonicum (Decapoda: Palaemonidae) in Brazil.

    Science.gov (United States)

    Moravec, Frantisek; Santos, Cláudia P

    2009-06-01

    Previously undescribed infective larvae of the cystidicolid nematode Pseudoproleptus sp. (probably conspecific with the nematode originally described as Heliconema izecksohni Fabio, 1982, a parasite of freshwater fish in Brazil), were found encapsulated in the hemocel of the Amazon river prawn Macrobrachium amazonicum (Heller) (Decapoda: Palaemonidae) from the natural canals on the Mexiana Island (Amazon River Delta), Pardá State, Brazil. The prevalence in prawns (body length 48-110 mm) examined in January and March 2008 (n = 44) was 32%, with an intensity of 1-6 (mean 2) larvae per crustacean. The nematode larvae (body length 19.7-25.7 mm), characterized by the cephalic end provided with a helmet-like cuticular structure having a thickened free posterior margin, are described based on light and scanning electron microscopy. Apparently prawns play a role as intermediate hosts for this nematode species. This is the first record of a larval representative of Cystidicolidae in South America and the first record of a species of Pseudoproleptus Khera, 1955, in the Neotropics. Heliconema izecksohni is transferred to Pseudoproleptus as Pseudoproleptus izecksohni (Fabio, 1982) n. comb.

  5. Isolation and characterization of nine polymorphic microsatellite markers for the deep-sea shrimp Nematocarcinus lanceopes (Crustacea: Decapoda: Caridea)

    Science.gov (United States)

    2013-01-01

    Background The shrimp Nematocarcinus lanceopes Bate, 1888 is found in the deep sea around Antarctica and sub-Antarctic islands. Previous studies on mitochondrial data and species distribution models provided evidence for a homogenous circum-Antarctic population of N. lanceopes. However, to analyze the fine-scale population genetic structure and to examine influences of abiotic environmental conditions on population composition and genetic diversity, a set of fast evolving nuclear microsatellite markers is required. Findings We report the isolation and characterization of nine polymorphic microsatellite markers from the Antarctic deep-sea shrimp species Nematocarcinus lanceopes (Crustacea: Decapoda: Caridea). Microsatellite markers were screened in 55 individuals from different locations around the Antarctic continent. All markers were polymorphic with 9 to 25 alleles per locus. The observed heterozygosity ranged from 0.545 to 0.927 and the expected heterozygosity from 0.549 to 0.934. Conclusions The reported markers provide a novel tool to study genetic structure and diversity in Nematocarcinus lanceopes populations in the Southern Ocean and monitor effects of ongoing climate change in the region on the populations inhabiting these. PMID:23448502

  6. El rol de Cyrtograpsus angulatus (Crustacea; Brachyura en los ciclos de vida de Microphallus szidati (Digenea; Microphallidae y Falsifilicollis chasmagnathi (Acanthocephala; Filicollidae: algunos aspectos de su ecologia parasitaria The role of Cytograpsus angulatus (Crustacea; Brachyura in the life cycles of Microphallus szidati (Digenea; Microphallidae and Falsifilicollis chasmagnathi (Acanthocephala; Filicollidae: some aspects of their ecological parasitology

    Directory of Open Access Journals (Sweden)

    Sergio R. Martorelli

    1989-12-01

    in the females (Spivak & Politis, in press appeared correlated with prevalence; c in the studied crabs the pervalence fo F. chasmagnathi is higher in males than in females; d the intensity did not appear correlated with size and sex of the intermediate host; e M. szidati and F. chasmagnathi are closely associated with the intermediate host; f C. angulatus and Himantopus melanurus Vieillot, 1817 (Aves; Recurvirostridae are reported as new intermediate and definitive hosts, respectively, for F. chasmagnathi; g Chasmagnathus granulata Dana, 1851 (Cruscea; Brachyura is reported as new intermediate host for M. szidati.

  7. Chlorine, temperature, and exposure duration effects of power plant effluents on juvenile blue crabs Callinectes sapidus and grass shrimp Palaemonetes pugio

    Energy Technology Data Exchange (ETDEWEB)

    Hall, L.W. Jr.; Burton, D.T.; Margrey, S.L.

    1979-07-01

    The effects of interacting Cl, temperature, and exposure conditions similar to those found in power plant effluents were assessed for the blue crab Callinectes sapidus and the grass shrimp Palemonetes pugio. Test organisms were exposed to total residual Cl (TRC) concentrations of 0.00, 0.15, and 0.30 mg/l in combination with temperatures 2, 6, and 10/sup 0/C above ambient for 0.08, 2.0, and 4.0 h. The TRC concentrations were decayed over a 1 to 1.5-h period to < 0.01 mg/l. Temperatures were decayed over a 4-h period to 2/sup 0/C above ambient. These conditions were used to simulate Cl and temperature conditions encountered in power plant discharge canals and near-field receiving streams. Regression model techniques were used to establish the interaction of Cl, temperature excess (..delta..T), and exposure duration as factors that caused death up to 36 h after the exposure period. Mortality increased for grass shrimp as the Cl concentration, ..delta..T, and length of exposure increased. A percentage mortality model for grass shrimp shows that (1) the response to Cl concentration alone and ..delta..T alone is proportional to the square root of Cl concentration and ..delta..T, (2) the effect of duration of exposure is linear, and (3) no second-order interactions occurred among the variables tested. No significant mortality was found for blue crabs for any of the variables tested.

  8. Comparative analysis of the proximate and elemental composition of the blue crab Callinectes sapidus, the warty crab Eriphia verrucosa, and the edible crab Cancer pagurus

    Directory of Open Access Journals (Sweden)

    Maurizio Zotti

    2016-02-01

    Full Text Available The proximate composition and element contents of claw muscle tissue of Atlantic blue crabs (Callinectes sapidus were compared with the native warty crab (Eriphia verrucosa and the commercially edible crab (Cancer pagurus. The scope of the analysis was to profile the chemical characteristics and nutritive value of the three crab species. Elemental fingerprints showed significant inter-specific differences, whereas non-significant variations in the moisture and ash contents were observed. In the blue crab, protein content was significantly lower than in the other two species, while its carbon content resulted lower than that characterizing only the warty crab. Among micro-elements, Ba, Cr, Cu, Li, Mn, Ni, and Pb showed extremely low concentrations and negligible among-species differences. Significant inter-specific differences were observed for Na, Sr, V, Ba, Cd and Zn; in particular, cadmium and zinc were characterized in the blue crab by concentrations significantly lower than in the other two species. The analysis of the available literature on the three species indicated a general lack of comparable information on their elemental composition. The need to implement extended elemental fingerprinting techniques for shellfish quality assessment is discussed, in view of other complementary profiling methods such as NMR-based metabolomics.

  9. PCR-based prevalence of a fatal reovirus of the blue crab, Callinectes sapidus (Rathbun) along the northern Atlantic coast of the USA.

    Science.gov (United States)

    Flowers, E M; Simmonds, K; Messick, G A; Sullivan, L; Schott, E J

    2016-06-01

    There is a need for more information on the relationship between diseases and fluctuations of wild populations of marine animals. In the case of Callinectes sapidus reovirus 1 (CsRV1, also known as RLV), there is a lack of baseline information on range, prevalence and outbreaks, from which to develop an understanding of population-level impacts. An RT-qPCR assay was developed that is capable of detecting 10 copies of the CsRV1 genome. In collaboration with state, federal and academic partners, blue crabs were collected from sites throughout the north-eastern United States to assess the northern range of this pathogen. In addition, archived crab samples from the Chesapeake Bay were assessed for CsRV1 by RT-qPCR and histology. PCR-based assessments indicate that CsRV1 was present at all but one site. Prevalence of CsRV1 as assessed by RT-qPCR was highly variable between locations, and CsRV1 prevalence varied between years at a given location. Mean CsRV1 prevalence as assessed by RT-qPCR was >15% each year, and peak prevalence was 79%. The wide geographic range and highly variable prevalence of CsRV1 indicate that more study is needed to understand CsRV1 dynamics and the role the virus plays in blue crab natural mortality. © 2015 The Authors Journal of Fish Diseases Published by John Wiley & Sons Ltd.

  10. Investigation of Petroleum and Wastewater Contaminants in Blue Crab, Callinectes sapidus, Megalopae in the Northern Gulf of Mexico using GC-MS

    Science.gov (United States)

    Chiasson, S. C.; Taylor, C.

    2016-02-01

    We collected blue crab, Callinectes sapidus, megalopae five days per week over a six-month period in 2010 and 2011 at multiple sites across the Northern Gulf of Mexico, and one site in 2013. We used GC-MS to investigate if we could detect evidence of petroleum hydrocarbons in megalopae, and if detected hydrocarbons could be linked to the DWH oil spill. We made a comparison between the distance from the oil slick to our collection sites and the concentration of long straight chain alkanes found in megalopae. We also compared levels of alkanes in megalopae between years. We then scanned the megalopae for presence of other contaminants using GC-MS. We detected long straight chain alkanes in megalopae in 2010 but not in 2011 or 2013. The highest levels of alkanes were found in Grand Isle, suggesting that the alkanes could possibly be sourced from the DWH oil spill. No Polycyclic Aromatic Hydrocarbons (PAHs) were detected, and we found no significant relationship between distance of oil slick to collection sites. Other componds were also detected, of which 4-nonylphenol, butylated hydroxytoluene, 2,4,6-tri-tertbutylphenol, and 2,4-di-tertbutylphenol exceeded the quantification limit.

  11. Stage-specific changes in calcium concentration in crustacean (Callinectes sapidus) Y-organs during a natural molting cycle, and their relation to the hemolymphatic ecdysteroid titer.

    Science.gov (United States)

    Chen, Hsiang-Yin; Dillaman, Richard M; Roer, Robert D; Watson, R Douglas

    2012-09-01

    Secretion of ecdysteroid molting hormones by crustacean Y-organs is suppressed by molt-inhibiting hormone (MIH). The suppressive effect of MIH on ecdysteroidogenesis is mediated by one or more cyclic nucleotide second messengers. In addition, existing data indicate that ecdysteroidogenesis is positively regulated (stimulated) by intracellular Ca(++). Despite the apparent critical role of calcium in regulating ecdysteroidogenesis, the level of Ca(++) in Y-organ cells has not been previously measured during a natural molting cycle for any crustacean species. In studies reported here, a fluorescent calcium indicator (Fluo-4) was used to measure Ca(++) levels in Y-organs during a molting cycle of the blue crab, Callinectes sapidus. Mean calcium fluorescence increased 5.8-fold between intermolt (C4) and stage D3 of premolt, and then dropped abruptly, reaching a level in postmolt (A) that was not significantly different from that in intermolt (P>0.05). The level of ecdysteroids in hemolymph of Y-organ donor crabs (measured by radioimmunoassay) showed an overall pattern similar to that observed for calcium fluorescence, rising from 2.9 ng/mL in intermolt to 357.1 ng/mL in D3 (P<0.05), and then dropping to 55.3 ng/mL in D4 (P<0.05). The combined results are consistent with the hypothesis that ecdysteroidogenesis is stimulated by an increase in intracellular Ca(++). Copyright © 2012 Elsevier Inc. All rights reserved.

  12. Systematic investigation of factors controlling supercritical fluid extraction (SFE) of spiked and aged PCBs from edible tissues of the blue crab (Callinectes sapidus).

    Science.gov (United States)

    Gaylor, Michael O; Harvey, Ellen; Hale, Robert C

    2015-01-01

    Systematic investigation of factors controlling supercritical fluid extraction (SFE) of spiked and naturally incurred (aged) PCBs from edible tissues of the Blue Crab (Callinectes sapidus) was undertaken. Effects of extraction pressure, temperature and time, CO2 flow rate and total volume, and collection temperature were assessed. Temperature dramatically impacted extraction efficiency, especially at lower pressures. Surprisingly, extraction of both spiked and aged PCBs was flow rate dependent, counter to prevailing views regarding the relative ease of SFE of spiked versus aged contaminants from environmental matrices. PCBs were optimally trapped on a 1:1 mixture of C18-modified and porous silica at 0°C and eluted with <2 mL isooctane at 90°C. A combined 10 min static/30 min dynamic extraction at 35.5 MPa and 150°C with a CO2 flow rate of 3 mL min(-1) yielded maximum (quantitative) recoveries of spiked and aged PCBs. Resulting solvent extracts required no cleanup and could be analyzed directly by halogen-selective GC with MS confirmation.

  13. Effects of temperature and salinity on prevalence and intensity of infection of blue crabs, Callinectes sapidus, by Vibrio cholerae, V. parahaemolyticus, and V. vulnificus in Louisiana.

    Science.gov (United States)

    Sullivan, Timothy J; Neigel, Joseph E

    2018-01-01

    Coastal marine and estuarine environments are experiencing higher average temperatures, greater frequency of extreme temperature events, and altered salinities. These changes are expected to stress organisms and increase their susceptibility to infectious diseases. However, beyond these generalities, little is known about how environmental factors influence host-pathogen relationships in the marine realm. We investigated the prevalence and intensity of infections by Vibrio cholerae, V. parahaemolyticus, and V. vulnificus in blue crabs, Callinectes sapidus, from Louisiana saltmarshes in relation to temperature and salinity. We evaluated relationships for single measurements taken at the time of collection and for more complex measurements representing accumulated exposure to physiologically-stressful environmental conditions for up to 31 days prior to collection. We found that: (1) prevalence of infection varied across the Louisiana coast, (2) prevalence of all three Vibrio species was influenced by temperature and salinity, and (3) measurements that represent accumulated exposure to extreme conditions are useful predictors of infection prevalence and can provide insights into underlying biological mechanisms. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Comparative analysis of the proximate and elemental composition of the blue crab Callinectes sapidus, the warty crab Eriphia verrucosa, and the edible crab Cancer pagurus.

    Science.gov (United States)

    Zotti, Maurizio; Coco, Laura Del; Pascali, Sandra Angelica De; Migoni, Danilo; Vizzini, Salvatrice; Mancinelli, Giorgio; Fanizzi, Francesco Paolo

    2016-02-01

    The proximate composition and element contents of claw muscle tissue of Atlantic blue crabs (Callinectes sapidus) were compared with the native warty crab (Eriphia verrucosa) and the commercially edible crab (Cancer pagurus). The scope of the analysis was to profile the chemical characteristics and nutritive value of the three crab species. Elemental fingerprints showed significant inter-specific differences, whereas non-significant variations in the moisture and ash contents were observed. In the blue crab, protein content was significantly lower than in the other two species, while its carbon content resulted lower than that characterizing only the warty crab. Among micro-elements, Ba, Cr, Cu, Li, Mn, Ni, and Pb showed extremely low concentrations and negligible among-species differences. Significant inter-specific differences were observed for Na, Sr, V, Ba, Cd and Zn; in particular, cadmium and zinc were characterized in the blue crab by concentrations significantly lower than in the other two species. The analysis of the available literature on the three species indicated a general lack of comparable information on their elemental composition. The need to implement extended elemental fingerprinting techniques for shellfish quality assessment is discussed, in view of other complementary profiling methods such as NMR-based metabolomics.

  15. Habitat selection of the pink shrimp Farfantepenaeus paulensis and the blue crab Callinectes sapidus in an estuary in southern Brazil: influence of salinity and submerged seagrass meadows

    Directory of Open Access Journals (Sweden)

    Vinicius Mendes Ruas

    Full Text Available This study was conducted in two estuarine inlets (Saco da Mangueira and Saco do Arraial at the Patos Lagoon estuary, southern Brazil. The changes in relative abundance and size of post-larvae and juvenile shrimp Farfantepenaeus paulensis and juvenile blue crab Callinectes sapidus were compared, considering the influence of salinity and the presence of submerged seagrass meadows. The analyses were performed using generalized linear models (GLM for abundance variations and ANOVA for variations on the size of individuals. The pink shrimp was more abundant at Saco da Mangueira, in seagrass meadows and areas of higher salinity. The blue crab was more abundant at Saco do Arraial and in lower levels of salinity. The importance of submerged vegetation for the blue crab lies in a preference of smaller crabs of the species for the seagrass meadows. It has been shown that these species choose different habitats in the estuary, and both the salinity and the presence of submerged seagrass meadows influence the selection of habitat.

  16. Effects of crude oil and oil/dispersant mixture on growth and expression of vitellogenin and heat shock protein 90 in blue crab, Callinectes sapidus, juveniles.

    Science.gov (United States)

    Chiasson, Susan C; Taylor, Caz M

    2017-06-30

    The 2010 Deepwater Horizon (DWH) oil spill in the northern Gulf of Mexico (NGOM) resulted in over 780million liters of crude oil spilling into Gulf waters. In an effort to disperse the oil, nearly 7.6million liters of dispersant was applied. Many commercially and recreationally important species reside in or near the area of the spill. The blue crab, Callinectes sapidus, is common in the NGOM and is both economically and ecologically important in this region. In this study, after exposing juvenile blue crabs to oil or a mixture of oil and dispersant we tested for relative expression of heat shock protein 90 (hsp90) and vitellogenin (vtg) by measuring their corresponding mRNA expression. We also monitored crabs over two molts to test for effects on growth. Expression of hsp90 was significantly downregulated, and we did not detect any effects of exposure to oil or oil/dispersant mixture on growth or vtg expression. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Crecimiento del cangrejo Callinectes danae (Decapoda: Portunidae de la Isla de Margarita, Venezuela

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    Jesylén Castillo

    2011-12-01

    Full Text Available Las especies del género Callinectes sostienen pesquerías importantes en varias partes del mundo. En el occidente de Venezuela, Callinectes sapidus es capturado con palangre y sostiene una pesquería artesanal importante en el Lago de Maracaibo; mientras que en la Isla de Margarita, Callinectes danae es capturado con nasa en zonas cercanas a lagunas costeras y su pesca está limitada por la oferta y la demanda. Estos recursos son de considerable importancia económica entre los crustáceos comestibles; sin embargo, pocos estudios han sido reportados sobre la dinámica poblacional y potencialidad pesquera en ambientes costeros de Venezuela que contribuyan a la toma de decisiones en la administración pesquera. En este documento se presenta información de los parámetros de crecimiento del cangrejo Callinectes danae Smith, 1869, con el fin de obtener un conocimiento detallado del patrón de crecimiento y lograr estimaciones más precisas sobre el tamaño de la población y su disponibilidad para la explotación. Se analizó una muestra constituida por 3 623 ejemplares capturados con nasa cangrejera por la flota artesanal, entre octubre 2007 y septiembre 2008. Se estableció la relación longitud-peso y se estimaron los parámetros de crecimiento de las curvas en longitud y peso del modelo de von Bertalanffy. La proporción sexual global mostró que no existe diferencia significativa entre machos y hembras (χ2=0.04, p>0.05. Los valores de las pendientes b entre machos y hembras presentaron diferencias significativas (ts=2.75, p<0.05 igualmente los interceptos a (ts=2.44, p<0.05; por lo que se estableció la relación longitud-peso por separado: P=7.48e-5*L2.98 para machos y P=1.21e-4*L2.87 para hembras, evidenciándose un crecimiento alométrico negativo en ambos sexos. Los parámetros de crecimiento quedaron establecidos en: L∞=134.80mm, P∞=166.04g y k=0.86/año para machos; L∞=122.35mm, P∞=118.45g y k=0.63/año para hembras. La

  18. Feeding habits of Stellifer rastrifer (Perciformes, Sciaenidae) at Guaratuba mangrove, Parana, Brazil

    OpenAIRE

    Chaves, Paulo de Tarso C.; Vendel, Ana Lúcia

    1998-01-01

    Feeding habits of Stellifer rastrifer (Perciformes, Sciaenidae) at Guaratuba mangrove, Parana, Brazil, were studied from February 1996 to February 1997. It was observed that its diet was based on invertebrates, mainly Decapoda non-Brachyura and Polychaeta. In a smaller proportion there were plants, Copepoda, Gammaridea and Mollusca. The level of contribution of each food item changed according to the season and the individual size. Such plasticity in feeding behaviour was similar to that desc...

  19. Descripción y distribución geográfica de una nueva especie para el archipiélago Juan Fernández: Platymera gaudichaudii (H. Milne-Edwards, 1837 (Decapoda, Calappidae

    Directory of Open Access Journals (Sweden)

    Marco A Retamal

    2013-11-01

    Full Text Available Se identifica una nueva especie en aguas del archipiélago Juan Fernández: Platymera gaudichaudii (Decapoda, Calappidae, ampliándose su distribución geográfica conocida en el hemisferio sur. Se entrega una diagnosis de la especie y se actualiza el status taxonómico de otras tres especies de decápodos en este archipiélago.

  20. Discovery and characterization of the Crustacean hyperglycemic hormone precursor related peptides (CPRP) and orcokinin neuropeptides in the sinus glands of the blue crab Callinectes sapidus using multiple tandem mass spectrometry techniques.

    Science.gov (United States)

    Hui, Limei; Cunningham, Robert; Zhang, Zichuan; Cao, Weifeng; Jia, Chenxi; Li, Lingjun

    2011-09-02

    The crustacean sinus gland (SG) is a well-defined neuroendocrine site that produces numerous hemolymph-borne agents including the most complex class of endocrine signaling molecules-neuropeptides. Via a multifaceted mass spectrometry (MS) approach, 70 neuropeptides were identified including orcokinins, orcomyotropin, crustacean hyperglycemic hormone (CHH) precursor-related peptides (CPRPs), red pigment concentrating hormone (RPCH), pigment dispersing hormone (PDH), proctolin, RFamides, RYamides, and HL/IGSL/IYRamide. Among them, 15 novel orcokinins, 9 novel CPRPs, 1 novel orcomyotropin, 1 novel Ork/Orcomyotropin-related peptide, and 1 novel PDH were de novo sequenced via collision induced dissociation (CID) from the SG of a model organism Callinectes sapidus. Electron transfer dissociation (ETD) was used for sequencing of intact CPRPs due to their large size and higher charge state. Capillary isoelectric focusing (CIEF) was employed for separation of members of the orcokinin family, which is one of the most abundant neuropeptide families observed in the SG. Collectively, our study represents the most complete characterization of neuropeptides in the SG and provides a foundation for future investigation of the physiological function of neuropeptides in the SG of C. sapidus.

  1. The trophic position of the Atlantic blue crab Callinectes sapidus Rathbun 1896 in the food web of Parila Lagoon (South Eastern Adriatic, Croatia: a first assessment using stable isotopes.

    Directory of Open Access Journals (Sweden)

    G. MANCINELLI

    2016-09-01

    Here, carbon and nitrogen isotopic signatures were measured in C. sapidus and other representative taxa of the benthic flora and fauna sampled in summer in the Parila Lagoon, a coastal basin located in the Neretva River estuary area (Croatia. d15N values of individual blue crab specimens were used to calculate their trophic position (TP , using the filter feeder mussel Mytilus galloprovincialis as the isotopic baseline. In general, the isotopic signature of C. sapidus was comparable with those characterizing invertebrate and fish predators (e.g., Hexaplex trunculus, Gobius niger. An average  trophic position of 3.73 was estimated for the blue crab population. However, a considerable inter-individual variation was observed in TP values, significantly related with the size of the specimens. Interestingly, a hump-shaped quadratic polynomial resulted the best fitting model for the data, characterized for small-size individuals by a positive body size-TP relationship, leveling or even turning negative for larger adult crabs. The interplay between environmental factors and ontogenetic diet shifts in determining the trophic position of the blue crab and, ultimately, its ecological impact on the benthic community of the Parila Lagoon is discussed.

  2. New functions of arthropod bursicon: inducing deposition and thickening of new cuticle and hemocyte granulation in the blue crab, Callinectes sapidus.

    Directory of Open Access Journals (Sweden)

    J Sook Chung

    Full Text Available Arthropod growth requires molt-associated changes in softness and stiffness of the cuticle that protects from desiccation, infection and injury. Cuticle hardening in insects depends on the blood-borne hormone, bursicon (Burs, although it has never been determined in hemolymph. Whilst also having Burs, decapod crustaceans reiterate molting many more times during their longer life span and are encased in a calcified exoskeleton, which after molting undergoes similar initial cuticle hardening processes as in insects. We investigated the role of homologous crustacean Burs in cuticular changes and growth in the blue crab, Callinectes sapidus. We found dramatic increases in size and number of Burs cells during development in paired thoracic ganglion complex (TGC neurons with pericardial organs (POs as neurohemal release sites. A skewed expression of Burs β/Burs α mRNA in TGC corresponds to protein contents of identified Burs β homodimer and Burs heterodimer in POs. In hemolymph, Burs is consistently present at ∼21 pM throughout the molt cycle, showing a peak of ∼89 pM at ecdysis. Since initial cuticle hardness determines the degree of molt-associated somatic increment (MSI, we applied recombinant Burs in vitro to cuticle explants of late premolt or early ecdysis. Burs stimulates cuticle thickening and granulation of hemocytes. These findings demonstrate novel cuticle-associated functions of Burs during molting, while the unambiguous and constant presence of Burs in cells and hemolymph throughout the molt cycle and life stages may implicate further functions of its homo- and heterodimer hormone isoforms in immunoprotective defense systems of arthropods.

  3. Investigating physiological, cellular and molecular effects in juvenile blue crab, Callinectus sapidus, exposed to field-collected sediments contaminated by oil from the Deepwater Horizon Incident.

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    Pie, Hannah V; Schott, Eric J; Mitchelmore, Carys L

    2015-11-01

    Juvenile blue crabs, Callinectus sapidus, were exposed for 31 days to six different sediments collected within the Pass a Loutre State Wildlife Management Area approximately 6 months or 1.5 years post-capping of the Macondo-252 well-head following the Deepwater Horizon (DWH) Incident. Based on forensic analysis to fingerprint for DWH oil, these sediments differed in their levels of DWH oil contamination, and included one reference sediment collected from a location with no detectable DWH oil present. The concentration of 50 individual parent and alkylation group polycyclic aromatic hydrocarbons (PAHs), saturated hydrocarbons (37 total), and total extractable hydrocarbons were determined in each sediment, as were biologically relevant metals, grain size distribution, percent total organic carbon, and percent total solids. Total concentrations of 50 PAHs (TPAH50) of initial treatment sediments ranged from 187 μg kg(-1) (reference site) to 2,086,458 μg kg(-1) (the highest DWH oil contaminated site). Multiple biological endpoints were measured including mortality, growth, and ecdysis. Additionally, early biomarkers of biological stress were examined in the hemolymph and hepatopancreas of crabs, including DNA damage (Comet assay) and expression of genes encoding Cu-metallothionein (CuMT), glutathione-S-transferase (GST), and manganese superoxide dismutase (MnSOD). Over the 31 day exposure, there were no treatment related mortalities in juvenile blue crabs. The overall growth and molting of the crabs were not substantially different between the various sediment exposures over the exposure period. Additionally, none of the early biomarkers of biological stress were correlated with PAH concentrations. Overall, juvenile blue crabs did not appear to be negatively impacted during the 31 day exposure by DWH oil contaminated sediments collected at least 6 months post-capping of the Macondo-252 well-head. Copyright © 2015. Published by Elsevier B.V.

  4. New functions of arthropod bursicon: inducing deposition and thickening of new cuticle and hemocyte granulation in the blue crab, Callinectes sapidus.

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    Chung, J Sook; Katayama, Hidekazu; Dircksen, Heinrich

    2012-01-01

    Arthropod growth requires molt-associated changes in softness and stiffness of the cuticle that protects from desiccation, infection and injury. Cuticle hardening in insects depends on the blood-borne hormone, bursicon (Burs), although it has never been determined in hemolymph. Whilst also having Burs, decapod crustaceans reiterate molting many more times during their longer life span and are encased in a calcified exoskeleton, which after molting undergoes similar initial cuticle hardening processes as in insects. We investigated the role of homologous crustacean Burs in cuticular changes and growth in the blue crab, Callinectes sapidus. We found dramatic increases in size and number of Burs cells during development in paired thoracic ganglion complex (TGC) neurons with pericardial organs (POs) as neurohemal release sites. A skewed expression of Burs β/Burs α mRNA in TGC corresponds to protein contents of identified Burs β homodimer and Burs heterodimer in POs. In hemolymph, Burs is consistently present at ∼21 pM throughout the molt cycle, showing a peak of ∼89 pM at ecdysis. Since initial cuticle hardness determines the degree of molt-associated somatic increment (MSI), we applied recombinant Burs in vitro to cuticle explants of late premolt or early ecdysis. Burs stimulates cuticle thickening and granulation of hemocytes. These findings demonstrate novel cuticle-associated functions of Burs during molting, while the unambiguous and constant presence of Burs in cells and hemolymph throughout the molt cycle and life stages may implicate further functions of its homo- and heterodimer hormone isoforms in immunoprotective defense systems of arthropods.

  5. Mercury content of blue crabs (Callinectes sapidus) from southern New England coastal habitats: Contamination in an emergent fishery and risks to human consumers.

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    Taylor, David L; Calabrese, Nicholas M

    2018-01-01

    Total mercury (Hg; ppm dry weight) was measured in blue crabs, Callinectes sapidus, collected from Narraganset Bay and adjacent coastal lagoons and tidal rivers (Rhode Island/Massachusetts, USA) from May to August 2006-2016. For juvenile crabs (21-79mm carapace width, CW), total Hg was significantly greater in chelae muscle tissue (mean±1 SD=0.32±0.21ppm; n=65) relative to whole bodies (0.21±0.16ppm; n=19), and irrespective of tissue-type, crab Hg was positively related to CW indicating bioaccumulation of the toxicant. Across a broader range of crab sizes (43-185mm CW; n=465), muscle Hg concentrations were significantly higher in crabs from the Taunton River relative to other locations (0.71±0.35ppm and 0.20±0.10ppm, respectively). Spatial variations in crab Hg dynamics were attributed to habitat-specific Hg burdens of their prey, including bivalves, gastropods, polychaetes, and shrimp. Prey Hg, in turn, was directly related to localized sediment Hg and methylmercury conditions. Biota-sediment accumulation factors for crabs and prey were negatively correlated with sediment organic content, verifying that organically-enriched substrates reduce Hg bioavailability. From a human health perspective, frequent consumption of crabs from the Taunton River may pose a human health risk (23% of legal-size crabs exceeded US EPA threshold level); thus justifying spatially-explicit Hg advisories for this species. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. D1-like dopamine receptors downregulate Na+-K+-ATPase activity and increase cAMP production in the posterior gills of the blue crab Callinectes sapidus.

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    Arnaldo, Francis B; Villar, Van Anthony M; Konkalmatt, Prasad R; Owens, Shaun A; Asico, Laureano D; Jones, John E; Yang, Jian; Lovett, Donald L; Armando, Ines; Jose, Pedro A; Concepcion, Gisela P

    2014-09-15

    Dopamine-mediated regulation of Na(+)-K(+)-ATPase activity in the posterior gills of some crustaceans has been reported to be involved in osmoregulation. The dopamine receptors of invertebrates are classified into three groups based on their structure and pharmacology: D1- and D2-like receptors and a distinct invertebrate receptor subtype (INDR). We tested the hypothesis that a D1-like receptor is expressed in the blue crab Callinectes sapidus and regulates Na(+)-K(+)-ATPase activity. RT-PCR, using degenerate primers, showed the presence of D1βR mRNA in the posterior gill. The blue crab posterior gills showed positive immunostaining for a dopamine D5 receptor (D5R or D1βR) antibody in the basolateral membrane and cytoplasm. Confocal microscopy showed colocalization of Na(+)-K(+)-ATPase and D1βR in the basolateral membrane. To determine the effect of D1-like receptor stimulation on Na(+)-K(+)-ATPase activity, intact crabs acclimated to low salinity for 6 days were given an intracardiac infusion of the D1-like receptor agonist fenoldopam, with or without the D1-like receptor antagonist SCH23390. Fenoldopam increased cAMP production twofold and decreased Na(+)-K(+)-ATPase activity by 50% in the posterior gills. This effect was blocked by coinfusion with SCH23390, which had no effect on Na(+)-K(+)-ATPase activity by itself. Fenoldopam minimally decreased D1βR protein expression (10%) but did not affect Na(+)-K(+)-ATPase α-subunit protein expression. This study shows the presence of functional D1βR in the posterior gills of euryhaline crabs chronically exposed to low salinity and highlights the evolutionarily conserved function of the dopamine receptors on sodium homeostasis. Copyright © 2014 the American Physiological Society.

  7. Cloning of prophenoloxidase from hemocytes of the blue crab, Callinectes sapidus and its expression and enzyme activity during the molt cycle.

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    Alvarez, Javier V; Chung, J Sook

    2013-11-01

    The arthropods cuticle undergoes dramatic morphological and biochemical changes from being soft to hardness through each molting process. Prophenoloxidase (PPO) known as a key enzyme in the arthropod innate immune system involved in the melanization reaction, has been related with the initial shell-hardening process, specifically in the sclerotization of the protein matrix in the new cuticle. Since hemocytes have been reported as the main PPO source in arthropods, the transport of hemocyte PPO into the newly laid, soft cuticle has been proposed for shell-hardening occurring during and immediately after ecdysis. In order to define the role of hemocyte PPO in the shell-hardening of crustaceans, the full-length cDNA sequence (2806 nt) of hemocytes PPO of the blue crab Callinectes sapidus (CasPPO-hemo) is isolated using degenerate PCR and 5'-3' RACE. CasPPO-hemo encodes a putative PPO (672 aa) showing three hemocyanin domains: N, M, and C in order and two copper binding sites (CuA & CuB). The sequence analysis identifies the putative CasPPO-hemo as zymogen which requires the cleavage at the N-terminus for its activation. Hemocyte extract (CasHLS) contains the PO, the activity of which depends on the in vitro activation of trypsin. The expression levels of CasPPO-hemo are kept constant during the molt cycle. The increase in the number of hemocytes at early premolt correlates with the elevated PO activity, while at late premolt, the increment in hemocyte numbers does not reflect on the PO activity. The functional importance of the changes in the levels of CasHLS-PO activity during molt cycle is discussed in relation to cuticle hardening process. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Feedback From Peripheral Musculature to Central Pattern Generator in the Neurogenic Heart of the Crab Callinectes sapidus: Role of Mechanosensitive Dendrites

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    García-Crescioni, Keyla; Fort, Timothy J.; Stern, Estee; Brezina, Vladimir

    2010-01-01

    The neurogenic heart of decapod crustaceans is a very simple, self-contained, model central pattern generator (CPG)-effector system. The CPG, the nine-neuron cardiac ganglion (CG), is embedded in the myocardium itself; it generates bursts of spikes that are transmitted by the CG's five motor neurons to the periphery of the system, the myocardium, to produce its contractions. Considerable evidence suggests that a CPG-peripheral loop is completed by a return feedback pathway through which the contractions modify, in turn, the CG motor pattern. One likely pathway is provided by dendrites, presumably mechanosensitive, that the CG neurons project into the adjacent myocardial muscle. Here we have tested the role of this pathway in the heart of the blue crab, Callinectes sapidus. We performed “de-efferentation” experiments in which we cut the motor neuron axons to the myocardium and “de-afferentation” experiments in which we cut or ligated the dendrites. In the isolated CG, these manipulations had no effect on the CG motor pattern. When the CG remained embedded in the myocardium, however, these manipulations, interrupting either the efferent or afferent limb of the CPG-peripheral loop, decreased contraction amplitude, increased the frequency of the CG motor neuron spike bursts, and decreased the number of spikes per burst and burst duration. Finally, passive stretches of the myocardium likewise modulated the spike bursts, an effect that disappeared when the dendrites were cut. We conclude that feedback through the dendrites indeed operates in this system and suggest that it completes a loop through which the system self-regulates its activity. PMID:19828726

  9. Production and characterization of films based on blends of chitosan from blue crab (Callinectes sapidus) waste and pectin from Orange (Citrus sinensis Osbeck) peel.

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    Baron, Ricardo Duran; Pérez, Latife Lúquez; Salcedo, Jesús Mejía; Córdoba, Luis Pérez; Sobral, Paulo José do Amaral

    2017-05-01

    The objective of this study was to develop and characterize films based on blends of chitosan and pectin, produced in laboratory scale, from industrial wastes. The chitosan was obtained by termoalcaline deacetylation of chitin, extracted from blue crab (Callinectes sapidus) waste and characterized according to degree of deacetylation (DD) and viscosimetric molecular weight (Mw); and pectin was extracted by conventional heating, from orange (Citrus sinensis Osbeck) peel and characterized according to degree of esterification (DE) and molecular weight (Mw). The Ch:P based films were prepared by the casting method in different Ch:P ratios [0: 100, 25:75, 50:50, 75:25 and 100:0], and compared to two controls [0:100 and 100:0], of commercial pectin and chitosan. Glycerol was used as a plasticizer at concentrations of 0.2g/g macromolecules. The addition of high concentrations of pectin in the formulations resulted in films with high solubility and an increase in moisture. No significant difference (P>0.05) in the degree of swelling (DS) and water vapor permeability (WVP) of the films was observed. Ch:P blend films were less stiff and therefore more elastic and flexible than films based on only one biopolymer. The control films presented better results in terms of color, being brighter and less opaque than other film formulations. These data suggest that chitosan or pectin obtained from agro-industrial waste is a potential matrix to produce biodegradable films for future food applications. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Systematic and evolutionary insights derived from mtDNA COI barcode diversity in the Decapoda (Crustacea: Malacostraca.

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    Joana Matzen da Silva

    Full Text Available BACKGROUND: Decapods are the most recognizable of all crustaceans and comprise a dominant group of benthic invertebrates of the continental shelf and slope, including many species of economic importance. Of the 17635 morphologically described Decapoda species, only 5.4% are represented by COI barcode region sequences. It therefore remains a challenge to compile regional databases that identify and analyse the extent and patterns of decapod diversity throughout the world. METHODOLOGY/PRINCIPAL FINDINGS: We contributed 101 decapod species from the North East Atlantic, the Gulf of Cadiz and the Mediterranean Sea, of which 81 species represent novel COI records. Within the newly-generated dataset, 3.6% of the species barcodes conflicted with the assigned morphological taxonomic identification, highlighting both the apparent taxonomic ambiguity among certain groups, and the need for an accelerated and independent taxonomic approach. Using the combined COI barcode projects from the Barcode of Life Database, we provide the most comprehensive COI data set so far examined for the Order (1572 sequences of 528 species, 213 genera, and 67 families. Patterns within families show a general predicted molecular hierarchy, but the scale of divergence at each taxonomic level appears to vary extensively between families. The range values of mean K2P distance observed were: within species 0.285% to 1.375%, within genus 6.376% to 20.924% and within family 11.392% to 25.617%. Nucleotide composition varied greatly across decapods, ranging from 30.8 % to 49.4 % GC content. CONCLUSIONS/SIGNIFICANCE: Decapod biological diversity was quantified by identifying putative cryptic species allowing a rapid assessment of taxon diversity in groups that have until now received limited morphological and systematic examination. We highlight taxonomic groups or species with unusual nucleotide composition or evolutionary rates. Such data are relevant to strategies for conservation of

  11. Systematic and evolutionary insights derived from mtDNA COI barcode diversity in the Decapoda (Crustacea: Malacostraca).

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    Matzen da Silva, Joana; Creer, Simon; dos Santos, Antonina; Costa, Ana C; Cunha, Marina R; Costa, Filipe O; Carvalho, Gary R

    2011-05-12

    Decapods are the most recognizable of all crustaceans and comprise a dominant group of benthic invertebrates of the continental shelf and slope, including many species of economic importance. Of the 17635 morphologically described Decapoda species, only 5.4% are represented by COI barcode region sequences. It therefore remains a challenge to compile regional databases that identify and analyse the extent and patterns of decapod diversity throughout the world. We contributed 101 decapod species from the North East Atlantic, the Gulf of Cadiz and the Mediterranean Sea, of which 81 species represent novel COI records. Within the newly-generated dataset, 3.6% of the species barcodes conflicted with the assigned morphological taxonomic identification, highlighting both the apparent taxonomic ambiguity among certain groups, and the need for an accelerated and independent taxonomic approach. Using the combined COI barcode projects from the Barcode of Life Database, we provide the most comprehensive COI data set so far examined for the Order (1572 sequences of 528 species, 213 genera, and 67 families). Patterns within families show a general predicted molecular hierarchy, but the scale of divergence at each taxonomic level appears to vary extensively between families. The range values of mean K2P distance observed were: within species 0.285% to 1.375%, within genus 6.376% to 20.924% and within family 11.392% to 25.617%. Nucleotide composition varied greatly across decapods, ranging from 30.8 % to 49.4 % GC content. Decapod biological diversity was quantified by identifying putative cryptic species allowing a rapid assessment of taxon diversity in groups that have until now received limited morphological and systematic examination. We highlight taxonomic groups or species with unusual nucleotide composition or evolutionary rates. Such data are relevant to strategies for conservation of existing decapod biodiversity, as well as elucidating the mechanisms and constraints

  12. Systematic and Evolutionary Insights Derived from mtDNA COI Barcode Diversity in the Decapoda (Crustacea: Malacostraca)

    Science.gov (United States)

    Matzen da Silva, Joana; Creer, Simon; dos Santos, Antonina; Costa, Ana C.; Cunha, Marina R.; Costa, Filipe O.; Carvalho, Gary R.

    2011-01-01

    Background Decapods are the most recognizable of all crustaceans and comprise a dominant group of benthic invertebrates of the continental shelf and slope, including many species of economic importance. Of the 17635 morphologically described Decapoda species, only 5.4% are represented by COI barcode region sequences. It therefore remains a challenge to compile regional databases that identify and analyse the extent and patterns of decapod diversity throughout the world. Methodology/Principal Findings We contributed 101 decapod species from the North East Atlantic, the Gulf of Cadiz and the Mediterranean Sea, of which 81 species represent novel COI records. Within the newly-generated dataset, 3.6% of the species barcodes conflicted with the assigned morphological taxonomic identification, highlighting both the apparent taxonomic ambiguity among certain groups, and the need for an accelerated and independent taxonomic approach. Using the combined COI barcode projects from the Barcode of Life Database, we provide the most comprehensive COI data set so far examined for the Order (1572 sequences of 528 species, 213 genera, and 67 families). Patterns within families show a general predicted molecular hierarchy, but the scale of divergence at each taxonomic level appears to vary extensively between families. The range values of mean K2P distance observed were: within species 0.285% to 1.375%, within genus 6.376% to 20.924% and within family 11.392% to 25.617%. Nucleotide composition varied greatly across decapods, ranging from 30.8 % to 49.4 % GC content. Conclusions/Significance Decapod biological diversity was quantified by identifying putative cryptic species allowing a rapid assessment of taxon diversity in groups that have until now received limited morphological and systematic examination. We highlight taxonomic groups or species with unusual nucleotide composition or evolutionary rates. Such data are relevant to strategies for conservation of existing decapod

  13. Occurrence and behaviour of Paromola cuvieri (Crustacea, Decapoda) in the Santa Maria di Leuca cold-water coral community (Mediterranean Sea)

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    Capezzuto, Francesca; Maiorano, Porzia; Panza, Michele; Indennidate, Antonella; Sion, Letizia; D'Onghia, Gianfranco

    2012-01-01

    Occurrence and behaviour of Paromola cuvieri (Crustacea, Decapoda) were recorded by means of the MEMO lander equipped with two digital cameras and deployed in the cold-water coral community of the Santa Maria di Leuca (Mediterranean Sea). A total of 14 individuals were observed at depths between 547 and 648 m; 10 in the coral habitat on coral mounds and 4 off the coral habitat on muddy bottoms. Thirteen specimens recorded were females, one male and all were shown to scavenge the bait. All the specimens carried a sponge on their exoskeleton using the fifth pereiopods. The specimens were distinguishable by the size and shape of the carried sponge. The present observations demonstrate both passive covering behaviour and active behaviour of discouraging approach and attack from competitors or predators, respectively. This study represents the first in situ documentation of Paromola cuvieri behaviour interacting with other deep-sea species in the Mediterranean Sea.

  14. First inventory of the Crustacea (Decapoda, Stomatopoda) of Juan de Nova Island with ecological observations and comparison with nearby islands in the Mozambique channel (Europa, Glorieuses, Mayotte)

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    Poupin, J.

    2016-04-01

    Crustacea Decapoda and Stomatopoda are inventoried for the first time in Juan de Nova Island, Iles Eparses, Mozambique channel. In total, 112 species are reported: 69 crabs, 28 anomurans, 11 shrimps, 3 mantis shrimps and 1 lobster. A comparison is made with nearby islands in the Mozambique channel: Glorieuses Islands (157 species), Europa Island (178 species), and Mayotte Island (505 species). The lower species richness at Juan de Nova is explained by the small size of the island and by the difficulties to collect the crustaceans on the reef flat hardly accessible at low tide. The crustaceans are listed by main habitats from land to outer reef (2-20 m). The presence of the coconut crab (Birgus latro), an endangered species vulnerable to human predation, is confirmed.

  15. Evaluation of lactic acid bacterium fermentation products and food-grade chemicals to control Listeria monocytogenes in blue crab (Callinectes sapidus) meat.

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    Degnan, A J; Kaspar, C W; Otwell, W S; Tamplin, M L; Luchansky, J B

    1994-01-01

    Fresh blue crab (Callinectes sapidus) meat was obtained from retail markets in Florida and sampled for viable Listeria monocytogenes. The pathogen was found in crabmeat in three of four different lots tested by enrichment and at levels of 75 CFU/g in one of the same four lots by direct plating. Next, crabmeat was steam sterilized, inoculated with a three-strain mixture of L. monocytogenes (ca. 5.5 log10 CFU/g), washed with various lactic acid bacterium fermentation products (2,000 to 20,000 arbitrary units [AU]/ml of wash) or food-grade chemicals (0.25 to 4 M), and stored at 4 degrees C. Counts of the pathogen remained relatively constant in control samples during storage for 6 days, whereas in crabmeat washed with Perlac 1911 or MicroGard (10,000 to 20,000 AU), numbers initially decreased (0.5 to 1.0 log10 unit/g) but recovered to original levels within 6 days. Numbers of L. monocytogenes cells decreased 1.5 to 2.7 log10 units/g of crabmeat within 0.04 day when washed with 10,000 to 20,000 AU of Alta 2341, enterocin 1083, or Nisin per ml. Thereafter, counts increased 0.5 to 1.6 log10 units within 6 days. After washing with food-grade chemicals, modest reductions (0.4 to 0.8 log10 unit/g) were observed with sodium acetate (4 M), sodium diacetate (0.5 or 1 M), sodium lactate (1 M), or sodium nitrite (1.5 M). However, Listeria counts in crabmeat washed with 2 M sodium diacetate decreased 2.6 log10 units/g within 6 days. In addition, trisodium phosphate reduced L. monocytogenes counts from 1.7 (0.25 M) to > 4.6 (1 M) log10 units/g within 6 days.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7944362

  16. The influence of temperature and salinity on mortality of recently recruited blue crabs, Callinectes sapidus, naturally infected with Hematodinium perezi (Dinoflagellata).

    Science.gov (United States)

    Huchin-Mian, Juan Pablo; Small, Hamish J; Shields, Jeffrey D

    2018-02-01

    The parasitic dinoflagellate Hematodinium perezi is highly prevalent in juvenile blue crabs, Callinectes sapidus, along the eastern seaboard of the USA. Although the parasite is known to kill adult crabs, the mortality rate of naturally infected juvenile crabs remains unknown. We analyzed the influence of temperature and salinity on the mortality of recently recruited blue crabs that were naturally infected with H. perezi. Over 492 juvenile crabs (infected, n = 282; uninfected controls, n = 210) were held individually in six-well plates and held at six temperatures (4, 10, 15, 20, 25, and 30 °C) or three salinities (5, 15, and 30 psu) for a maximum of 90 days. Mortality of infected crabs was 10 times higher at elevated temperatures (25 and 30 °C) and salinity (30 psu) compared to uninfected control treatments. By contrast, infected crabs exposed to mild temperatures (10, 15, and 20 °C) showed a high survival (>80%), no different than uninfected control treatments. Infected crabs at the lowest temperature (4 °C) exhibited a high mortality, but the intensity of infection was lower than in the other temperature treatments. In addition, this study revealed the optimal temperature (25 °C) and salinity (30 psu) for H. perezi to progress in its life cycle leading to sporulation in juvenile crabs; 31.6% (19/60) of crabs held under these conditions released dinospores of H. perezi after 10 days. Crabs held at other temperatures did not release dinospores over the time course of the experiment. Infected crabs were capable of molting and in most cases molted at the same frequency as uninfected crabs serving as controls. The mortality observed in this study indicates that early benthic juveniles will experience significant mortality due to H. perezi with increasing ocean temperatures and that this mortality may be a significant factor in the recruitment of blue crabs to high salinity regions. Copyright © 2018. Published by Elsevier Inc.

  17. Molecular cloning of a putative crustacean hyperglycemic hormone (CHH) isoform from extra-eyestalk tissue of the blue crab (Callinectes sapidus), and determination of temporal and spatial patterns of CHH gene expression.

    Science.gov (United States)

    Zheng, Junying; Chen, Hsiang-Yin; Choi, Cheol Young; Roer, Robert D; Watson, R Douglas

    2010-11-01

    Crustacean hyperglycemic hormone (CHH) is a polypeptide neurohormone involved in regulation of multiple physiological processes. We report here the cloning from thoracic ganglia of the blue crab (Callinectes sapidus) a cDNA (CsCHH-2) encoding a putative CHH isoform (CsCHH-2). CsCHH-2 is structurally similar to a putative preproCHH (CsCHH-1) previously cloned from eyestalk ganglia of C. sapidus. The two preprohormones possess an identical signal peptide and CHH precursor related peptide, but differ in the mature CHH polypeptide. An analysis by RT-PCR of the tissue distribution of CsCHH-1 and CsCHH-2 revealed the former is restricted to eyestalk neural ganglia, while the latter is widely distributed among tissues. The type of CHH transcript present in eyestalk and thoracic ganglia did not vary as a function of the molt cycle. An assessment of transcript abundance in tissues of intermolt crabs showed the abundance of the CsCHH-1 transcript in eyestalk ganglia far exceeds the abundance of the CsCHH-2 transcript in extra-eyestalk tissue. An assessment of transcript abundance during a molt cycle showed CsCHH-1 transcript abundance in eyestalk ganglia was low during intermolt, rose during premolt, reaching a peak in D(3), then fell prior to molting, and remained low during postmolt. By contrast, CsCHH-2 transcript abundance in thoracic ganglia was low during intermolt, rose sharply during D(2), then dropped in D(3) and remained low during postmolt. The results are consistent with the hypothesis that CsCHH-1 and CsCHH-2 differ with respect to physiological function. Copyright © 2010 Elsevier Inc. All rights reserved.

  18. Acute toxicity, accumulation and tissue distribution of copper in the blue crab Callinectes sapidus acclimated to different salinities: In vivo and in vitro studies

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    De Martinez Gaspar Martins, Camila [Programa de Pos-Graduacao em Ciencias Fisiologicas - Fisiologia Animal Comparada, Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), Campus Carreiros, Av. Italia km 8, 96201-900, Rio Grande, RS (Brazil); Barcarolli, Indianara Fernanda; Menezes, Eliana Jaime de; Mussoi Giacomin, Marina [Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), Campus Carreiros, Av. Italia km 8, 96201-900, Rio Grande, RS (Brazil); Wood, Chris M. [McMaster University, Department of Biology, 1280 Main Street West, Hamilton, ON, L8S 4K1 (Canada); Bianchini, Adalto, E-mail: adaltobianchini@furg.br [Programa de Pos-Graduacao em Ciencias Fisiologicas - Fisiologia Animal Comparada, Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), Campus Carreiros, Av. Italia km 8, 96201-900, Rio Grande, RS (Brazil); Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), Campus Carreiros, Av. Italia km 8, 96201-900, Rio Grande, RS (Brazil)

    2011-01-17

    In vivo and in vitro studies were performed to evaluate acute toxicity, organ-specific distribution, and tissue accumulation of copper in Callinectes sapidus acclimated to two different experimental salinities (2 and 30 ppt). Blue crabs were quite tolerant to copper. Acute dissolved copper toxicity (96-h LC{sub 50} and its corresponding 95% confident interval) was higher at salinity 2 ppt (5.3 (3.50-8.05) {mu}M Cu) than at 30 ppt (53.0 (27.39-102.52) {mu}M Cu). The difference between salinities can be completely explained based on the water chemistry because it disappeared when 96-h LC{sub 50} values were expressed as the free Cu{sup 2+} ion (3.1 (1.93-4.95) {mu}M free Cu at 2 ppt versus 5.6 (2.33-13.37) {mu}M free Cu at 30 ppt) or the Cu{sup 2+} activity (1.4 (0.88-2.26) {mu}M Cu activity at 2 ppt versus 1.7 (0.71-4.07) {mu}M Cu activity at 30 ppt). The relationships between gill Cu burden and % mortality were very similar at 2 and 30 ppt, in accord with the Biotic Ligand Model. In vivo experiments showed that copper concentration in the hemolymph is not dependent on metal concentration in the surrounding medium at either experimental salinity. They also showed that copper flux into the gills is higher than into other tissues analyzed, and that anterior and posterior gills are similarly important sites of copper accumulation at both experimental salinities. In vitro experiments with isolated-perfused gills showed that there is a positive relationship between copper accumulation in this tissue and the metal concentration in the incubation media for both anterior and posterior gills. A similar result was observed at both low and high salinities. Furthermore, in vitro experiments showed that copper accumulation in posterior gills is also positively and strongly dependent on the incubation time with copper. Gill copper accumulation occurred at a lower rate in the first 2 h of metal exposure, increasing markedly after this 'steady-state' period. This finding

  19. Acute toxicity, accumulation and tissue distribution of copper in the blue crab Callinectes sapidus acclimated to different salinities: in vivo and in vitro studies.

    Science.gov (United States)

    Martins, Camila De Martinez Gaspar; Barcarolli, Indianara Fernanda; de Menezes, Eliana Jaime; Giacomin, Marina Mussoi; Wood, Chris M; Bianchini, Adalto

    2011-01-17

    In vivo and in vitro studies were performed to evaluate acute toxicity, organ-specific distribution, and tissue accumulation of copper in Callinectes sapidus acclimated to two different experimental salinities (2 and 30 ppt). Blue crabs were quite tolerant to copper. Acute dissolved copper toxicity (96-h LC(50) and its corresponding 95% confident interval) was higher at salinity 2 ppt (5.3 (3.50-8.05) μM Cu) than at 30 ppt (53.0 (27.39-102.52) μM Cu). The difference between salinities can be completely explained based on the water chemistry because it disappeared when 96-h LC(50) values were expressed as the free Cu(2+) ion (3.1 (1.93-4.95) μM free Cu at 2 ppt versus 5.6 (2.33-13.37) μM free Cu at 30 ppt) or the Cu(2+) activity (1.4 (0.88-2.26) μM Cu activity at 2 ppt versus 1.7 (0.71-4.07) μM Cu activity at 30 ppt). The relationships between gill Cu burden and % mortality were very similar at 2 and 30 ppt, in accord with the Biotic Ligand Model. In vivo experiments showed that copper concentration in the hemolymph is not dependent on metal concentration in the surrounding medium at either experimental salinity. They also showed that copper flux into the gills is higher than into other tissues analyzed, and that anterior and posterior gills are similarly important sites of copper accumulation at both experimental salinities. In vitro experiments with isolated-perfused gills showed that there is a positive relationship between copper accumulation in this tissue and the metal concentration in the incubation media for both anterior and posterior gills. A similar result was observed at both low and high salinities. Furthermore, in vitro experiments showed that copper accumulation in posterior gills is also positively and strongly dependent on the incubation time with copper. Gill copper accumulation occurred at a lower rate in the first 2h of metal exposure, increasing markedly after this "steady-state" period. This finding was corroborated by a significant

  20. Abbreviation of larval development and extension of brood care as key features of the evolution of freshwater Decapoda.

    Science.gov (United States)

    Vogt, Günter

    2013-02-01

    The transition from marine to freshwater habitats is one of the major steps in the evolution of life. In the decapod crustaceans, four groups have colonized fresh water at different geological times since the Triassic, the freshwater shrimps, freshwater crayfish, freshwater crabs and freshwater anomurans. Some families have even colonized terrestrial habitats via the freshwater route or directly via the sea shore. Since none of these taxa has ever reinvaded its environment of origin the Decapoda appear particularly suitable to investigate life-history adaptations to fresh water. Evolutionary comparison of marine, freshwater and terrestrial decapods suggests that the reduction of egg number, abbreviation of larval development, extension of brood care and lecithotrophy of the first posthatching life stages are key adaptations to fresh water. Marine decapods usually have high numbers of small eggs and develop through a prolonged planktonic larval cycle, whereas the production of small numbers of large eggs, direct development and extended brood care until the juvenile stage is the rule in freshwater crayfish, primary freshwater crabs and aeglid anomurans. The amphidromous freshwater shrimp and freshwater crab species and all terrestrial decapods that invaded land via the sea shore have retained ocean-type planktonic development. Abbreviation of larval development and extension of brood care are interpreted as adaptations to the particularly strong variations of hydrodynamic parameters, physico-chemical factors and phytoplankton availability in freshwater habitats. These life-history changes increase fitness of the offspring and are obviously favoured by natural selection, explaining their multiple origins in fresh water. There is no evidence for their early evolution in the marine ancestors of the extant freshwater groups and a preadaptive role for the conquest of fresh water. The costs of the shift from relative r- to K-strategy in freshwater decapods are traded

  1. Species diversity and distribution of freshwater crabs (Decapoda: Pseudothelphusidae inhabiting the basin of the Rio Grande de Térraba, Pacific slope of Costa Rica

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    Luis Rólier Lara

    2013-09-01

    Full Text Available During the last decades, knowledge on biodiversity of freshwater decapods has increased considerably; however, information about ecology of these crustaceans is scarce. Currently, the freshwater decapod fauna of Costa Rica is comprised by representatives of three families (Caridea: Palaemonidae and Atyidae; Brachyura: Pseudothelphusidae. The present study aims to describe the species diversity and distribution of freshwater crabs inhabiting the basin of the Rio Grande de Térraba, Pacific slope of Costa Rica, where the Instituto Costarricense de Electricidad (ICE plans to implement one of the largest damming projects in the region. Samples were collected in 39 locations at an altitude ranging from 20 to 1,225 m. Sampling was carried out during several months in 2007, 2009 and 2010. We collected a total of 661 crabs, comprising eight species of Pseudothelphusidae of three genera, representing 53% of the 15 pseudothelphusid crab species currently recorded from Costa Rica. The most common species was Ptychophallus paraxanthusi followed by P. tristani. Freshwater crabs were more frequently encountered in the middle-low region of the basin (between 311 and 600 m and less frequently in the medium-high basin (between 601 and 1,225 m. Ptychophallus paraxanthusi showed the widest distribution and was collected in altitudes ranging from 20 to 700 m. The Rio Grande de Térraba region can be considered as a relatively small, but highly diverse system. Therefore, any alteration of the basin of Rio Grande de Térraba, and especially the possible construction of a hydroelectric power plant, needs to be carefully analyzed to mitigate the damaging effects of this project on the freshwater crabs. More ecological information about freshwater crabs from Costa Rica and the Central American region are needed to reach a first reasonable overview on the ecological role of these decapods in freshwater systems.

  2. Biology of three Malacostraca (Decapoda) in a Mediterranean lagoon with particular emphasis on the effect of rapid environmental changes on the activity (catchability) of the species

    Science.gov (United States)

    Crivelli, A. J.

    1982-12-01

    The activity of three Malacostraca (Decapoda), Crangon crangon, Carcinus aestuarii and Paleamon squilla, was studied following rapid environmental changes in a Mediterranean lagoon linked temporarily with the sea. Nekton was sampled every 2-3 days using fixed fyke nets. A canonical analysis and a stepwise multiple regression were computed to determine which factor(s) can predict or account for the catch of these three decapods. In spite of rapid environmental changes no exceptional mortality was observed in each of the three species. Temperature is the environmental factor with major impact on the activity of C. crangon. Activity of P. squilla is controlled by water depth. At a depth of less than 20 cm two factors, salinity and phase of the moon, have a significant influence on the activity of this species. At depths deeper than 20 cm the activity is inhibited. There is only a weak relationship between the catch of C. aestuarii and salinity. Additional data on the life cycle of these species are given. The limitations of a monthly sampling period are discussed and the need for more short-term, consecutive sampling periods is stressed in order to understand the dynamics of systems with rapid environmental fluctuations.

  3. A new species of hermit crab, Diogenes heteropsammicola (Crustacea, Decapoda, Anomura, Diogenidae, replaces a mutualistic sipunculan in a walking coral symbiosis.

    Directory of Open Access Journals (Sweden)

    Momoko Igawa

    Full Text Available Symbiont shift is rare in obligate mutualisms because both the partners are reciprocally dependent on and specialized to each other. In the obligate accommodation-transportation mutualism between walking corals and sipunculans, however, an unusual saltatory symbiont shift was discovered. In shallow waters of southern Japan, an undescribed hermit crab species was found living in corallums of solitary scleractinian corals of the genera Heterocyathus and Heteropsammia, replacing the usual sipunculan symbiont. We described the hermit crab as a new species Diogenes heteropsammicola (Decapoda, Anomura, Diogenidae, and explored its association with the walking corals. This hermit crab species obligately inhabits the coiled cavity of the corals, and was easily distinguished from other congeneric species by the exceedingly slender chelipeds and ambulatory legs, and the symmetrical telson. Observations of behavior in aquaria showed that the new hermit crab, like the sipunculan, carries the host coral and prevents the coral from being buried. This is an interesting case in which an organism phylogenetically distant from Sipuncula takes over the symbiotic role in association with a walking coral. The hermit crab species is unique in that its lodging is a living solitary coral that grows with the hermit crab in an accommodation-transportation mutualism.

  4. ABUNDANCE AND MORPHOMETRIC RELATIONSHIPS OF AMAZON SHRIMP - Macrobrachium amazonicum (HELLER 1862 (DECAPODA, PALAEMONIDAE - IN AN AMAZON ESTUARY - NORTH COAST OF BRAZIL

    Directory of Open Access Journals (Sweden)

    Bianca Bentes da Silva

    2016-12-01

    Full Text Available From Macrobrachium amazonicum (Heller, 1862 (Decapoda, Palaemonidae abundance in monthly collections in the Amazon estuary and on Mosqueiro Island (State of Pará, Brazil between April 2006 and August 2007, we verified that the site mentioned is favorable to the development of the species, since there is a significant number of young and adults specimens throughout the year, whose abundance is significantly higher in the dry season. Icoaraci was the site of great contribution in the total of specimens caught, relating this productivity to the large amount of organic matter in suspension. In this study, we found the largest M. amazonicum specimen ever collected, comparing to those mentioned by available scientific literature. The individual was a female caught on Combu Island with 44.72 mm carapace length or 18.45 cm total length. Relations of body mass (g vs. carapace length (mm for males, females and sexes together had negative allometry, which can be associated to the gonadal maturation cycle of the species. All other morphometric relationships showed positive allometry. Keywords. carapace; bio-ecology; population dynamics.

  5. A novel putative lipoprotein receptor (CasLpR) in the hemocytes of the blue crab, Callinectes sapidus: cloning and up-regulated expression after the injection of LPS and LTA.

    Science.gov (United States)

    Tsutsui, Naoaki; Chung, J Sook

    2012-03-01

    The full-length cDNA encoding a putative lipoprotein receptor (CasLpR) was isolated from the hemocytes of Callinectes sapidus using 5' and 3' RACEs. The open reading frame for CasLpR contains a precursor of putative CasLpR consisting of 1710 amino acid residues including 22 amino acid residues of the signal peptide (22 amino acids). Mature CasLpR (1688 amino acids with 5.6% of phosphorylation sites) has multiple, putative functional domains: five low-density lipoprotein receptor domains in the N-terminus, and a G-protein-coupled receptor proteolysis site domain and a 7 transmembrane receptor (secretin family) domain in the C-terminus. To date, there are no proteins with a similar domain structure in the GenBank. The expression pattern of CasLpR was exclusive in hemocytes among all tested tissues obtained from a juvenile female at intermolt stage: brain, eyestalk ganglia, pericardial organs, and thoracic ganglia complex (nervous system); hepatopancreas (digestive system); heart, artery and hemocytes (circulatory system); gill and antennal gland (excretory system), hypodermis; and Y-organ (endocrine organ). There was no CasLpR expression in the ovary of an adult female. A putative function of CasLpR was examined after challenges of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in vivo using qRT-PCR assays. Animals at 24 h after injection of LPS or LTA up-regulated the expression of CasLpR in hemocytes by ∼3.5 and 1.4 folds, respectively, compared to the controls that received saline injection. LPS challenge also caused the greatest increment (∼55 folds) of heat shock protein 90 (Hsp90) expression in these samples. These data indicate that putative CasLpR and CasHsp90 may be involved in the defense system or the stress response of C. sapidus. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. Dynamic patterns of zooplankton transport and migration in Catuama Inlet (Pernambuco, Brazil, with emphasis on the decapod crustacean larvae Patrones dinámicos de transporte y migración de zooplancton en el estuario Catuama (Pernambuco, Brasil, con énfasis en las larvas de crustáceos decápodos

    Directory of Open Access Journals (Sweden)

    Ralf Schwamborn

    2008-01-01

    Full Text Available The objective of this study was to quantify and to model zooplankton transport with emphasis on decapod crustacean larvae. Sampling was carried out at three depths with a plankton pump coupled to a 300-/¿m mesh. Current data were obtained with an ADCP. Our data showed the existence of vertically and horizontally heterogeneous current and transport fields. We identified 27 groups of Decapoda (larvae of Sergestidae, Porcellanidae, Upogebiidae, Cari dea, Brachyura. Most of the species and larval phases showed characteristic vertical migration patterns, in phase with the diurnal tidal cycles, thus enhancing retention or export from the estuary.Esta investigación tiene como objetivo cuantificar y modelar el transporte de zooplancton, con énfasis en las larvas de Crustacea Decapoda. El muestreo se realizó a tres profundidades con una bomba de plancton acoplada a una red con malla de 300 /¿m. Se obtuvieron los datos de corrientes con un ADCP. Los datos mostraron la existencia de campos de corrientes y de transporte vertical y horizontal heterogéneos. Se identificaron 27 grupos de Decapoda (larvas de Sergestidae, Porcellanidae, Upogebiidae, Caridea, Brachyura. La mayoría de las especies y fases larvales mostraron patrones de migración vertical característicos, en fase con los ciclos de la marea diurnos, favoreciendo la retención o exportación del estuario.

  7. Distribución espacial de Ocypode quadrata (Decapoda: Ocypodidae en ocho playas de la zona norte oriental de Cuba

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    Frank A. Ocaña

    2012-09-01

    Full Text Available Uno de los organismos más comunes en las playas arenosas son los miembros del género Ocypode conocidos como cangrejos fantasmas, están ampliamente distribuidos en las regiones tropicales y subtropicales; además tienen un importante papel en la transferencia de energía en diferentes niveles tróficos. El objetivo del presente trabajo es dar a conocer aspectos relacionados con la variación espacial de la densidad de O. quadrata en playas con diferente nivel de influencia humana en la costa norte oriental de Cuba. En mayo de 2010, fueron contadas un total de 355 galerías en 360 unidades de muestreo. La composición por clases de tallas fue de 237 galerías de jóvenes y 118 de adultos. Del total de galerías contadas el 74% ocuparon el nivel P1, 20% el nivel P2 y 6% el nivel P3. La densidad varió entre 0.06-0.52galerías/m2. A pesar del nivel de influencia humana, la mayoría de las playas no mostró diferencias significativas en la densidad de galerías (Scheffé, p>0.05. La densidad de individuos en la zona superior de resaca del oleaje es mayor (Scheffé, pSpatial distribution of Ocypode quadrata (Decapoda: Ocypodidae in eight beaches of NorthEastern Cuba. Studies on the ecology of Ocypode quadrata have been mostly carried out in the Northern and Southern part of its distribution range. In despite that this species is common in Cuban beaches, there are no quantitative studies regarding its abundance and spatial distribution. The aim of this study was to report some aspects about the spatial variation of O. quadrata density in sandy beaches, with different levels of human influence, in the North coast of Eastern Cuba. For this, on May 2010, eight beaches with different levels of human influence were surveyed. On each beach, the number of crabs burrows were counted in 45 quadrats of 4m2 located in three different strata (P1, P2 and P3. According to burrow opening diameter, crabs were separated into young and adults forms. To determine the

  8. Influencia del ciclo lunar en la captura de langosta espinosa Panulirus argus (Decapoda: Palinuridae en el Golfo de Batabanó, Cuba

    Directory of Open Access Journals (Sweden)

    Alexander Lopeztegui

    2011-03-01

    Full Text Available Muchos procesos biológicos tales como los comportamientos reproductivos y migratorios se han asociado con los ciclos de la luna. Con el objetivo de determinar la relación entre las capturas de langosta espinosa Panulirus argus (Decapoda: Palinuridae y el ciclo lunar, en la zona del Golfo de Batabanó, se correlacionaron los niveles de iluminación nocturna del ciclo lunar (INT con la captura diaria por barco (CDB durante siete meses lunares correspondientes al período de pesca del 2002. Los datos de captura provinieron de las tres empresas pesqueras que realizan su actividad en la zona del Golfo de Batabanó. La relación entre el ciclo lunar y las capturas de langostas fue examinada mediante varios procedimientos estadísticos. Los primeros gráficos realizados, cualitativos, mostraron que no existe relación entre las tasas de captura y el ciclo lunar. Los coeficientes de Spearman calculados, modularmente menores que 0.1 en todos los casos, demostraron cuantitativamente que no existe correlación entre las mencionadas variables. La prueba de Kruskal-Wallis detectó diferencias sólo en una de las tres áreas estudiadas. Las correlaciones cruzadas no detectaron significación en ninguna de las zonas, con coeficientes de correlación cruzada modularmente inferiores a 0.1. A diferencia de lo reportado por otros autores, las capturas de P. argus y el ciclo lunar no presentan correlación significativa en el Golfo de Batabanó.Influence of lunar cycle on catches of spiny lobster Panulirus argus (Decapoda: Palinuridae in the Gulf of Batabanó, Cuba. Many biological processes such as reproductive and migratory behaviours have been associated with moon cycles. In this study, the nocturnal light levels associated with lunar cycle (INT were correlated with daily catch rate of lobster P. argus, during seven lunar months of 2002 fishing period, to determine a possible relationship between these variables. The lobster catches were obtained from three

  9. Evolution of life history traits in Asian freshwater prawns of the genus Macrobrachium (Crustacea: Decapoda: Palaemonidae) based on multilocus molecular phylogenetic analysis.

    Science.gov (United States)

    Wowor, Daisy; Muthu, Victor; Meier, Rudolf; Balke, Michael; Cai, Yixiong; Ng, Peter K L

    2009-08-01

    Freshwater prawns of the genus Macrobrachium are free-living decapod crustaceans that are commonly encountered in tropical streams and lakes. We present a molecular phylogenetic analysis of the diverse Southeast and East Asian fauna based on >3 kb sequence data from three nuclear and two mitochondrial markers for almost 50% of the described fauna. We reconstruct the phylogenetic relationships and track the evolution of key life history traits. Our tree suggests that the last common ancestor of the Asian Macrobrachium laid numerous small eggs and had prolonged larval development ("PLD") in saline coastal waters after which the adults matured in freshwater habitats. We also argue for five independent losses of the marine larval phase to yield five clades of species that develop entirely in freshwater and have fewer and larger eggs than the species with PLD. These species have either semi-abbreviated (two origins) with at least one free-swimming stage or abbreviated larval development ("ALD": three origins) which lack free-swimming larvae. A Shimodaira-Hasegawa test rejects all trees that would imply a single loss of the marine larval phase, but alternative and equally parsimonious optimizations exist that imply a smaller number of losses. However, these scenarios would require the re-acquisition of free-swimming larvae. A concentrated-change test supports Pereira and Garcia's [Pereira, G.A., Garcia, J.V., 1995. Larval development of Macrobrachium reyesi Pereira (Decapoda, Palaemonidae), with a discussion on the origin of abbreviated development in palaemonids. J. Crust. Biol. 15, 117-133] hypothesis of a significant correlation between living in freshwater and the origin of semi-abbreviated and abbreviated larval development. Our phylogenetic tree also reveals that Asian Macrobrachium have independently become cavernicolous at least twice, and invaded the highly acidic waters of freshwater and peat swamps two or three times.

  10. Nearshore Community Studies of Neah Bay, Washington

    Science.gov (United States)

    1988-06-01

    Leptochelia dubia 0.1 Isopoda ɘ.1 Idorea sp. 0.1 Capreflidea ɘ.1 Caprella sp. 0.1 Decapoda- Brachyura 0.5 Pinnotheridae 0.1 Crangon sp. 0.3 Chironornidae...Candea- larvae 0.1 0.5 Crangon sp.-larvae 0.3 0.6 36.0 Dec:Voda-larvae 0.2 0.5 2.0 50.2 2.3 Brachyura-larvae 1.4 0.1 0.6 1.9 1.1 ɘ.1 Cancer sp...seine collections at Baadah Point and Crown Z in May. Planktonic prey, principally fish and sand shrimp ( Crangon sp.) larvae and calanoid copepods

  11. Annotated checklist of the decapod crustaceans of the Gulf of Oman, northwestern Indian Ocean.

    Science.gov (United States)

    Naderloo, Reza; Ebrahimnezhad, Saeed; Sari, Alireza

    2015-10-09

    The decapod crustaceans of the Gulf of Oman have been documented based on the published literature and new sampling along the Iranian coast between 2005 and 2015. A total of 121 species were collected along the Iranian coast, of which 43 are new records for the Gulf of Oman. The Decapoda of the Gulf is currently represented by 258 species belonging to five infraorders: Axiidea, Achelata, Anomura, Brachyura, and Caridea. Brachyura, with 176 species, are the best represented group, followed by Anomura and Caridea with 42 and 17 species, respectively. The least diverse groups are Achelata, with five species, and Axiidea, with three. On the basis of the available information, the northern (Iranian) coast with 189 species is more diverse than the southern (United Arab Emirates and Oman) coast with 134 species.

  12. TECHNICAL NOTE A NEW, VOLUNTEER-BASED, COST EFFECTIVE METHOD FOR ZOOLOGICAL MAPPING: THE PHOTO IDENTIFICATION OF FRESHWATER CRAYFISH (CRUSTACEA: DECAPODA SPECIES AND THE IMPORTANCE OF VOLUNTEERS IN CRAYFISH RESEARCH

    Directory of Open Access Journals (Sweden)

    PUKY M.

    2006-01-01

    Full Text Available The publication of the European Crayfish Atlas within the framework of the CRAYNET programme is a major breakthrough in Decapoda mapping in Europe. The current data base contains information from approximately 60-70% of the 50 km × 50 km squares covering the continent. A new method to improve this situation further is the use of photo identification involving volunteers in the collection of distribution data. The crayfish identification ability of two potential user groups, astacologists and biology teachers/students, was tested using the questionnaire given in Figure 2. One picture (Astacus leptodactylus male was correctly identified by everyone, the others were recognised by 59-94% (astacologists and 30-88% (non-crayfish specialised biology teachers and students. The first European Decapoda photo identification survey proved that the necessary expertise exists in the continent to determine crayfish species from pictures and also that the quality of the pictures is less important than the presence of key details. Photo identification is a low cost and environmental-friendly approach but it also needs special considerations. Volunteers need to be supported in several ways, such as by providing precise and interesting educational material in an easily understandable language describing e.g. the key parts of the body to photograph. However, the general use of digital cameras and even mobile phones/cameras and the Internet is an effective way to launch such surveys as it gives, on one hand, an opportunity for the specialists to check, and if necessary correct, the field identification of less experienced people and, on the other hand, it provides a much larger data base than what is available now by using the data collected by students, conservationists, scouts or other volunteers.

  13. [Proceedings of the 5th Symposium on Mesozoic and Cenozoic Decapod Crustaceans, Krakow, Poland, 2013: A tribute to Pál Mihály Müller / R.H.B. Fraaije, M. Hyžný, J.W.M. Jagt, M. Krobicki & B.W.M. van Bakel (eds.)]: A new genus and species of raninoidian crab (Decapoda, Brachyura) from the Lower Cretaceous of Colombia, South America

    NARCIS (Netherlands)

    Luque, J.

    2014-01-01

    A new raninoidian crab, Bellcarcinus aptiensis gen. nov., sp. nov., is described from the upper Aptian of Colombia. The new genus displays a combination of traits seen among some ancient species within the Necrocarcinidae and Orithopsidae, obscuring its family placement. This taxon represents the

  14. CRUSTACEA: DECAPODA: THALASSINIDEA

    African Journals Online (AJOL)

    November-January). 2. A maximum ... Although species of Callianassa have a world-wide distribution very few studies of their breeding and ..... The author acknowledges the financial assistance of the Department of Nature Conservation of the Cape ...

  15. Thalassinidea, Decapoda, Crustacea

    African Journals Online (AJOL)

    1986-07-23

    Jul 23, 1986 ... Oxygen consumption experiments were performed on the thalassinid prawn, Callianassa kraussi Stabbing, using an open flow system coupled to a Radiometer electrode and a continuous recorder. The effect of starvation, sex, body size, salinity, temperature, seasons and reduced oxygen tensions on the ...

  16. The presence of an insulin-like androgenic gland factor (IAG) and insulin-like peptide binding protein (ILPBP) in the ovary of the blue crab, Callinectes sapidus and their roles in ovarian development.

    Science.gov (United States)

    Huang, Xiaoshuai; Ye, Haihui; Chung, J Sook

    2017-08-01

    Insulin-like androgenic gland factor (IAG) that is produced by the male androgenic gland (AG), plays a role in sexual differentiation and maintenance of male secondary sex characteristics in decapod crustaceans. With an earlier finding of IAG expression in a female Callinectes sapidus ovary, we aimed to examine a putative role of IAG during the ovarian development of this species. To this end, the full-length cDNA sequence of the ovarian CasIAG (termed CasIAG-ova) has been isolated. The predicted mature peptide sequence of CasIAG-ova is identical to that of the IAG from the AG, except in their signal peptide regions. The CasIAG-ova contains an alternative initiation codon (UUG) as the start codon, which suggests that the translational regulation of CasIAG-ova may differ from that of the IAG from AG. To define the function of CasIAG-ova, the expressions of CasIAG-ova as well as its putative binding protein, insulin-like peptide binding protein (ILPBP), are measured in the ovaries at various developmental stages obtained from different seasons. Season affects both CasIAG and ILPBP expression in the ovary. Overall, summer females at earlier ovarian stages contain high levels of CasIAG and ILPBP than spring or fall females. These findings indicate that CasIAG-ova and CasILPBP may be involved in the ovarian development. When comparing the levels of CasIAG and CasILPBP in the ovary, the latter are much higher (∼10-10000 fold) than the former. Expression patterns of CasILPBP differ from those of CasIAG-ova during ovarian development and by season, suggesting that ILPBP may have an additional role in ovarian development rather than a function of a putative binding protein of IAG. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Molecular cloning of a plasma membrane Ca²⁺ ATPase (PMCA) from Y-organs of the blue crab (Callinectes sapidus), and determination of spatial and temporal patterns of PMCA gene expression.

    Science.gov (United States)

    Chen, Hsiang-Yin; Roer, Robert D; Watson, R Douglas

    2013-06-10

    Existing data indicate that a stage-specific increase in intracellular free Ca(2+) stimulates ecdysteroid production by crustacean molting glands (Y-organs). The concentration of Ca(2+) in cytosol is controlled mainly by proteins intrinsic to the plasma membrane and to the membranes of organelles. Several families of proteins are involved, including Ca(2+) channels, Ca(2+) pumps (ATPases), and Ca(2+) exchangers. The family of Ca(2+) pumps includes plasma membrane calcium ATPases (PMCAs). As a step toward understanding the involvement of calcium signaling in regulation of ecdysteroidogenesis, we used a PCR-based cloning strategy (RT-PCR followed by 3'- and 5'-RACE) to clone from Y-organs of the blue crab (Callinectes sapidus) a cDNA encoding a putative PMCA. The 4292 base pair (bp) cDNA includes a 3510 bp open reading frame encoding a 1170-residue protein (Cas-PMCA). The conceptually translated protein has a relative molecular mass of 128.8×10(3) and contains all signature domains of an authentic PMCA, including ten transmembrane domains and a calmodulin binding site. The predicted membrane topography of Cas-PMCA is as expected for an authentic PMCA protein. A phylogenetic analysis of nonredundant amino acid sequences of PMCA proteins from different species showed Cas-PMCA clusters with other arthropod PMCA proteins. An assessment of tissue distribution showed the Cas-PMCA transcript to be broadly distributed in both neural and non-neural tissues. Studies using quantitative real-time PCR revealed stage-specific changes in Cas-PMCA abundance during the molting cycle, with peak expression occurring during premolt stage D2, a pattern consistent with the hypothesis that Cas-PMCA functions to maintain cellular Ca(2+) homeostasis in Y-organs. Copyright © 2013 Elsevier B.V. All rights reserved.

  18. The smell of moulting: N-acetylglucosamino-1,5-lactone is a premoult biomarker and candidate component of the courtship pheromone in the urine of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Kamio, Michiya; Schmidt, Manfred; Germann, Markus W; Kubanek, Julia; Derby, Charles D

    2014-04-15

    Female blue crabs (Callinectes sapidus) in their pubertal moult stage release unidentified sex pheromone molecules in their urine, causing males to respond with courtship behaviours including a display called courtship stationary paddling and a form of precopulatory guarding called cradle carry. We hypothesized that pheromones are mixtures of molecules and are more concentrated in urine of pubertal premoult females compared with other moulting stages and thus that these molecules are biomarkers (i.e. metabolites that can be used as an indicator of some biological state or condition) of pubertal premoult females. We tested this hypothesis by combining bioassay-guided fractionation and biomarker targeting. To evaluate the molecular mass of the putative pheromone by bioassay-guided fractionation, we separated urine from pubertal premoult females and intermoult males by ultrafiltration into three molecular mass fractions. The 1000 Da fraction of female urine induced male courtship stationary paddling, but none of the fractions of male urine did. Thus, female urine contains molecules of <1000 Da that stimulate courtship behaviours in males. Biomarker targeting using nuclear magnetic resonance (NMR) spectral analysis of the 500-1000 Da fraction of urine from premoult and postmoult males and females revealed a premoult biomarker. Purification, nuclear magnetic resonance, mass spectrometry and high pressure liquid chromatography analysis of this premoult biomarker identified it as N-acetylglucosamino-1,5-lactone (NAGL) and showed that it is more abundant in urine of premoult females and males than in urine of either postmoult or juvenile females and males. NAGL has not been reported before as a natural product or as a molecule of the chitin metabolic pathway. Physiological and behavioural experiments demonstrated that blue crabs can detect NAGL through their olfactory pathway. Thus, we hypothesize that NAGL is a component of the sex pheromone and that it acts in

  19. Gene transcripts encoding hypoxia-inducible factor (HIF) exhibit tissue- and muscle fiber type-dependent responses to hypoxia and hypercapnic hypoxia in the Atlantic blue crab, Callinectes sapidus.

    Science.gov (United States)

    Hardy, Kristin M; Follett, Chandler R; Burnett, Louis E; Lema, Sean C

    2012-09-01

    Hypoxia inducible factor (HIF) is a transcription factor that under low environmental oxygen regulates the expression of suites of genes involved in metabolism, angiogenesis, erythropoiesis, immune function, and growth. Here, we isolated and sequenced partial cDNAs encoding hif-α and arnt/hif-β from the Atlantic blue crab, Callinectes sapidus, an estuarine species that frequently encounters concurrent hypoxia (low O(2)) and hypercapnia (elevated CO(2)). We then examined the effects of acute exposure (1h) to hypoxia (H) and hypercapnic hypoxia (HH) on relative transcript abundance for hif-α and arnt/hif-β in different tissues (glycolytic muscle, oxidative muscle, hepatopancreas, gill, and gonads) using quantitative real-time RT-PCR. Our results indicate that hif-α and arnt/hif-β mRNAs were constitutively present under well-aerated normoxia (N) conditions in all tissues examined. Further, H and HH exposure resulted in both tissue-specific and muscle fiber type-specific effects on relative hif-α transcript abundance. In the gill and glycolytic muscle, relative hif-α mRNA levels were significantly lower under H and HH, compared to N, while no change (or a slight increase) was detected in oxidative muscle, hepatopancreas and gonadal tissues. H and HH did not affect relative transcript abundance for arnt/hif-β in any tissue or muscle fiber type. Thus, in crustaceans the HIF response to H and HH appears to involve changes in hif transcript abundance, with variation in hif-α and arnt/hif-β transcriptional dynamics occurring in both a tissue- and muscle fiber type-dependent manner. Copyright © 2012 Elsevier Inc. All rights reserved.

  20. Environmental factors influencing the distribution of three species within the genus Persephona Leach, 1817 (Crustacea, Decapoda, Leucosiidae in two regions on the northern coast of São Paulo State, Brazil

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    Rafaela T Pereira

    2014-05-01

    Full Text Available Patterns of spatio-temporal distribution of Brachyura are determined by the interaction among life history traits, inter and intraspecific relationships, as well as by the variation of abiotic factors. This study aimed to characterize patterns of spatio-temporal distribution of Persephona lichtensteinii, Persephona mediterranea and Persephona punctata in two regions of the northern coast of São Paulo State, southeastern region of Brazil. Collections were done monthly from July 2001 to June 2003 in Caraguatatuba and Ubatuba, using a shrimp fishery boat equipped with double-rig nets. The patterns of species distribution were tested by means of redundancy analysis (RDA and generalized linear mixed models (GLMM in relation to the recorded environmental factors (BT: bottom temperature, BS: bottom salinity, OM: organic matter and granulometry (Phi. The most influent environmental factor over the species distribution was the Phi, and the ascendant order of influence was P. lichtensteinii, P. punctata and P. mediterranea. The greater abundance of P. mediterranea showed a conservative pattern of distribution for the genus in the sampled region. The greater occurrence of P. punctata and P. lichtensteinii, in distinct transects than those occupied by P. mediterranea, seems to be a strategy to avoid competition among congeneric species, which is related to the substratum specificity.

  1. Relação energia: proteína dietária para pós-larvas de Macrobrachium amazonicum (Crustacea, Decapoda - DOI: 10.4025/actascianimsci.v25i2.1979 Dietary energy: protein ratio for Macrobrachium amazonicum (Crustacea, Decapoda- DOI: 10.4025/actascianimsci.v25i2.1979

    Directory of Open Access Journals (Sweden)

    Dario Rocha Falcon

    2003-04-01

    Full Text Available Esta pesquisa foi realizada no Laboratório de Nutrição de Organismos Aquáticos (AquaNutri da FMVZ - Unesp - Campus de Botucatu, unidade integrada ao CaUnesp. Teve por objetivo avaliar o desempenho de pós-larvas de Macrobrachium amazonicum (Crustacea, Decapoda, submetidas a dietas purificadas, contendo 3 níveis de proteína bruta (30%; 35% e 40% e 2 níveis de energia bruta (3200 e 3600kcal/kg de ração, em um delineamento inteiramente casualizado, sistema fatorial 3x2, com 5 repetições. Pós-larvas com peso médio inicial de 280±12mg, provenientes do Setor de Carcinicultura do CaUnesp, foram mantidas em 30 aquários de 80 litros, em um sistema de recirculação de água (1,0L/min com temperatura controlada. Os resultados demonstraram, após 60 dias, a importância do balanço e a interação da energia e da proteína para as pós-larvas. Pode-se concluir que as rações com 35,0% de proteína bruta e 3600kcal de EB/kg proporcionam melhores respostas de ganho de peso e conversão alimentar para o M. amazonicum; que rações com 30,0% de proteína bruta e 3600kcal de EB/kg proporcionam melhores taxas de sobrevivência; e que as melhores respostas de crescimento são obtidas quando as rações apresentam a relação energia:proteína de 10,28:1,0.This experiment was conducted at the Aquatic Nutrition Organisms Laboratory (AquaNutri, integrated unit to CaUnesp. The aim of this study was to evaluate the growth performance of Macrobrachium amazonicum (Crustacea, Decapoda, post larvae. Post larvae were fed diet containing different levels of protein and energy. A factorial experiment with three levels of protein (30.0; 35.0 and 40.0 crude protein and two levels of energy (3200 and 3600 gross energy kcal/kg was carried out on a completely randomly design, with five replicates. 360 post larvae were randomly stocked, average weight of 280±12mg, into 30 aquaria, 80L each aquarium. Albumin-gelatin diet was used. Weight gain, feed conversion and

  2. Larval development of Sesarma catenata Ortmann (Brachyura ...

    African Journals Online (AJOL)

    1986-11-17

    Nov 17, 1986 ... 202. S.-Afr.Tydskr.Dierk.1987,22(3). Table 2 Segmentation and setation of appendages of Sesarma catenata zoeal stages; A = aesthetacs; S = simple; SP = sparcely plumose; HP = highly plumose; D. = denticulate; hp = hirsute proximal; pdt = plumodenticulate terminal; all symbols ex- cept 'A' refer to setae.

  3. Pesquería y parámetros biométricos de los cangrejos del género Calappa (Brachyura: Calappidae en el oriente de Venezuela

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    José J Alió

    2005-09-01

    para ser comercializadas, mientras que el ejemplar vivo es devuelto al mar, para fines prácticos una relación común para las tres especies, que permitiría convertir el peso de las quelas a peso total del ejemplar sería: Peso total = 3 x peso quelas. No se observa una tendencia descendente significativa en la relación entre las capturas por unidad de esfuerzo y el esfuerzo. La variación de las capturas tiende a seguir la del esfuerzo de pesca, con una tendencia descendente de ambos parámetros en los últimos años. No se encontraron hembras ovadas en las tres especies, por lo que pudieran ocurrir migraciones reproductivas hacia aguas más profundas durante los meses de octubre a enero, cuando la abundancia de los cangrejos es menor en el área de estudio. Se recomienda que la práctica de extraer una sola quela, devolviendo el resto del animal vivo al mar, debe promoverse entre los pescadores, mientras que debería evitarse la extracción de quelas en las hembras.Fishery and biometrics of genus Calappa crabs (Brachyura: Calappidae in northeastern Venezuela. The box crabs, genus Calappa, are important crab species landed by the industrial trawl fleet in Venezuela. These crabs have a wide distribution in the country, from the Gulf of Venezuela to the Orinoco River, but major landings take place in such gulf, near Margarita Island and northern Sucre State. Average annual landing in the eastern region was 69 t during 1970-2001, with a maximum of 221 t. For this study a sample of 2 398 box crabs was collected by observers on board of industrial trawl vessels, which operated in eastern Venezuela from 1994 to 2000. Three species were found in the landings, Calappa sulcata and C. flammea, each represented 47% of the sample, and C. nítida, which accounted for 5% of the sample. Sex ratio was strongly biased towards males in C. sulcata (2.8:1 and in C. flammea (1.9:1, while it was only slightly biased in C. nitida (1.4:1. Due to its large size, C. sulcata is of great

  4. Molt-inhibiting hormone stimulates vitellogenesis at advanced ovarian developmental stages in the female blue crab, Callinectes sapidus 2: novel specific binding sites in hepatopancreas and cAMP as a second messenger

    Science.gov (United States)

    Zmora, Nilli; Sagi, Amir; Zohar, Yonathan; Chung, J Sook

    2009-01-01

    The finding that molt-inhibiting hormone (MIH) regulates vitellogenesis in the hepatopancreas of mature Callinectes sapidus females, raised the need for the characterization of its mode of action. Using classical radioligand binding assays, we located specific, saturable, and non-cooperative binding sites for MIH in the Y-organs of juveniles (J-YO) and in the hepatopancreas of vitellogenic adult females. MIH binding to the hepatopancreas membranes had an affinity 77 times lower than that of juvenile YO membranes (KD values: 3.22 × 10-8 and 4.19 × 10-10 M/mg protein, respectively). The number of maximum binding sites (BMAX) was approximately two times higher in the hepatopancreas than in the YO (BMAX values: 9.24 × 10-9 and 4.8 × 10-9 M/mg protein, respectively). Furthermore, MIH binding site number in the hepatopancreas was dependent on ovarian stage and was twice as high at stage 3 than at stages 2 and 1. SDS-PAGE separation of [125I] MIH or [125I] crustacean hyperglycemic hormone (CHH) crosslinked to the specific binding sites in the membranes of the J-YO and hepatopancreas suggests a molecular weight of ~51 kDa for a MIH receptor in both tissues and a molecular weight of ~61 kDa for a CHH receptor in the hepatopancreas. The use of an in vitro incubation of hepatopancreas fragments suggests that MIH probably utilizes cAMP as a second messenger in this tissue, as cAMP levels increased in response to MIH. Additionally, 8-Bromo-cAMP mimicked the effects of MIH on vitellogenin (VtG) mRNA and heterogeneous nuclear (hn) VtG RNA levels. The results imply that the functions of MIH in the regulation of molt and vitellogenesis are mediated through tissue specific receptors with different kinetics and signal transduction. MIH ability to regulate vitellogenesis is associated with the appearance of MIH specific membrane binding sites in the hepatopancreas upon pubertal/final molt. PMID:19583849

  5. Estructura de tallas y crecimiento de los cangrejos Callinectes arcuatus y C. bellicosus (Decapoda: Portunidae en la laguna costera Las Guásimas, México

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    Luis Hernández

    2007-03-01

    Full Text Available Presentamos información sobre la estructura de tallas, la relación ancho-peso y los parámetros de crecimiento de las jaibas Callinectes arcuatus y C. bellicosus. Las muestras fueron recolectadas mensualmente con una red de arrastre tipo camaronera durante el día y la noche desde marzo 1998 hasta febrero 2000 en una laguna costera del Golfo de California. C. bellicosus (n= 878 fue más abundante que C. arcuatus (n= 357 y su intervalo de talla de 8.4- 166 mm ancho del caparazón (AC y 9-130 mm AC, respectivamente. Ambas poblaciones son representadas principalmente por jóvenes (75 mm AC entre 37-75 mm AC y adultos entre 76- 90 mm AC. La relación ancho-peso mostró que los machos crecen más que las hembras en ambas especies y se observó una tendencia de crecimiento isométrico. Los parámetros de crecimiento para C. arcuatus estimados con el modelo von Bertalanffy fueron: K= 0.84 año-1, L∞= 140 a -0.12 mm para C. arcuatus, y K= 0.9 año -1, L∞= 169 a -0.11 mm para C. bellicosus. Estos resultados mostraron que la edad relativa a la cual se alcanza el crecimiento máximo es entre los tres y cuatro años de edad para ambas especies.Size frequency distribution and growth of the crabs Callinectes arcuatus and C. bellicosus (Decapoda: Portunidae in Las Guásimas coastal lagoon, Mexico. Information on size frequency distribution, the width-weight relation and growth parameters of the crabs Callinectes arcuatus and C. bellicosus is presented. The data comes from samples taken with a trawl net both day and night on a monthly basis from March 1998 to February 2000 in a coastal lagoon from Gulf of California. C. bellicosus (n= 878 was more abundant than C. arcuatus (n= 357 and its size frequency distribution presented carapace width CW ranges of 8.4-166 mm and 9-130 mm respectively. Both populations were mainly represented by juveniles (75 mm in CW ranging from 37 to 75 mm in CW, and adults (>75 mm in CW between 76 and 90 mm in CW. In both species

  6. Crecimiento y reproducción del camarón Atya margaritacea (Decapoda: Atyidae en el Río Presidio, Sinaloa, México

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    Jesús Sánchez Palacios

    2008-06-01

    Full Text Available Se estudiaron el crecimiento y reproducción de de Atya margaritacea, con base en 542 organismos capturados en cinco sitios distribuidos desde la parte media de la cuenca hasta la desembocadura del río Presidio (Sinaloa, NW de México. La recolecta se realizó mediante el empleo de una red Surber, recolectándose organismos cuya longitud total y peso total variaron de 15 a 96 mm y 0.1 a 25.2 g respectivamente. La proporción macho:hembra fue 1.96:1 y los machos alcanzaron las mayores tallas y pesos. La relación entre la longitud total (Lt y el peso total (Pt se estableció mediante el modelo Pt = 1.02x10-5 (Lt3.2089 para los machos y por Pt = 2.29x10-5 (Lt3.0159 para las hembras. El crecimiento en machos resultó alométricamente positivo mientras que en hembras fue isométrico. La regresión entre la longitud total y longitud cefalotórax (Lc fue Log Lc = 1.1118 (Log Lt- 0.6087 en machos y Log Lc = 0.9945 (Log Lt- 0.4321 en hembras. El crecimiento relativo entre estas partes del cuerpo resultó alométrico positivo en machos e isométrico en hembras, indicando un claro dimorfismo sexual de estos organismos. Hembras ovígeras se presentaron en la época de lluvias (julio a noviembre. La fecundidad absoluta varió de 1 860 a 22 400 huevos en hembras de 43 y 59 mm de longitud y de 1.9 a 6.0 g de peso. La ecuación que relacionó el número de huevos con la longitud y peso, fue Fec = 8.3x10-7 (Lt5.8053 y Fec = 732 (Pt1.836 respectivamente.Growth and reproduction of the shrimp Atya margaritacea (Decapoda: Atyidae in Río Presidio, Sinaloa, Mexico. Some growth and reproduction parameters of the population of the shrimp Atya margaritacea in Presidio River (Sinaloa, NW Mexico were studied using 542 organisms collected with a Surber net in five sampling locations distributed from mid- to low river. Total lengths and gross weights ranged from 15 to 96 mm and 0.1 to 25.2 g. The male:female ratio was 1.96:1 and males had larger sizes and weights. The

  7. Efecto tóxico de DDT y endosulfan en postlarvas de camarón blanco, Litopenaeus vannamei (Decapoda:Penaeidaede Chiapas,México

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    Vicente Castro-Castro

    2005-06-01

    Full Text Available Con el fin de conocer la toxicidad del DDT y endosulfan sobre postlarvas de camarón blanco (Litopenaeus vannaei, se realizaron pruebas de toxicidad aguda en condiciones de laboratorio por 168 h, con temperatura de 29 ± 1 °C, salinidad de 3 ± 1 ‰ y pH en 8 ± 1.Se calculó la concentración letal media (LC50 , la LC50 "incipiente", los tiempos medios de muerte (LT50 , la Máxima Concentración Aceptable del Tóxico (MACT y el "Nivel de Seguridad" (LS; así mismo, en los organismos sobrevivientes se determinó la concentración a la que el crecimiento de los organismos se reduce en un 5 y 50% (CE5 y CE50 . Se evaluaron además las alteraciones en el consumo de oxígeno. El DDT fue 3 veces más tóxico que el endosulfan; sin embargo, los organismos resultaron ser muy sensibles a ambos compuestos. La tasa de crecimiento de las postlarvas disminuyó en un 80 y 50% para el DDT y endosulfan respectivamente. La baja resistencia de las postlarvas al DDT y endosulfan, y las concentraciones de estos compuestos en la laguna, sugieren que si se diera un ingreso adicional de estos plaguicidas al sistema, es muy probable un potencial impacto en la producción de camarón del sistemaToxic efect of DDT and endosulfan in white shrimp postlarvae Litopenaeus vannamei (Decapoda: Penaeidae from Chiapas, Mexico .We analized acute toxicity in white shrimp (Litopenaeus vannamei postlarvae exposed to two chlorinated pesticides, DDT and endosulfan, under laboratory conditions during 168 hours, with controlled temperature (29 ± 1°C, salinity (3 ± 1 ‰ and pH (8 ± 1. Median lethal concentrations (LC50 , "incipient" LC50, median lethal time (LT50 the "maximum acceptable concentration of the toxic compound" (MACT and "the safety level" (SL were determined. The concentration of the compounds at which organism growth was reduced by 5 and 50% (EC5 and EC50 , as well as changes in oxygen consumption patterns were determined in the surviving postlarvae.They were very

  8. Feeding habits of Stellifer rastrifer (Perciformes, Sciaenidae at Guaratuba mangrove, Parana, Brazil

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    Paulo de Tarso C. Chaves

    1998-08-01

    Full Text Available Feeding habits of Stellifer rastrifer (Perciformes, Sciaenidae at Guaratuba mangrove, Parana, Brazil, were studied from February 1996 to February 1997. It was observed that its diet was based on invertebrates, mainly Decapoda non-Brachyura and Polychaeta. In a smaller proportion there were plants, Copepoda, Gammaridea and Mollusca. The level of contribution of each food item changed according to the season and the individual size. Such plasticity in feeding behaviour was similar to that described to some fish populations from other estuaries, and could be an indicator of the high level of instability presented by this kind of ecosystem.A alimentação do cangulo ou canguá, Stellifer rastrifer, foi estudada no manguezal da Baía de Guaratuba entre fevereiro 1996 e fevereiro 1997. Constatou-se que a dieta está baseada em invertebrados, sobretudo Decapoda Brachyura e Polychaeta. Secundariamente, registram-se vegetais superiores, Copepoda, Gammaridea e Mollusca. O grau de participação dos itens varia com a estação e o tamanho dos indivíduos. Tal plasticidade no hábito alimentar, também conhecida em outras populações de peixes estuarinos, pode ser considerada como mais um indicador do grau de instabilidade encontrado nestes ambientes.

  9. Padrão de deslocamento do camarão marinho Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae nas fases clara e escura ao longo de 24 horas Displacement pattern of marine shrimp Litopenaeus vannamei (Boone, 1931 (Crustacea, Decapoda, Penaeidae in light and dark phases during a 24-hour period

    Directory of Open Access Journals (Sweden)

    Cibele S. Pontes

    2006-03-01

    Full Text Available O padrão diário de atividade de Litopenaeus vannamei (Boone, 1931 (Crustacea, Decapoda, Penaeidae é ainda pouco entendido. Com o objetivo de fornecer subsídios para otimizar o manejo nas fazendas de cultivo através do estudo do comportamento deste animal, foram desenvolvidos experimentos com juvenis (7,57 ± 1,01 g, mantidos em aquários (30 l. Para registro da atividade, os aquários foram submetidos a fotoperíodo artificial (fase do claro e do escuro, 12h:12 h, sendo monitorados 16 camarões (33 m² em cada fase, durante 20 dias, em janelas de 15 min a cada hora. A alimentação foi fornecida em horários aleatórios, sendo registrados os deslocamentos horizontal e vertical (focal contínuo e os comportamentos de natação e exploração (focal instantâneo. Os deslocamentos horizontal e vertical dos camarões foram mais acentuados na fase escura, predominando o comportamento de natação. A exploração do substrato ocorreu em ambas fases, acentuando-se entre cinco e sete horas após o acendimento das luzes.Litopenaeus vannamei (Boone, 1931 daily behavior pattern is still poorly understood. With the aim of optimizing the management of shrimp farms through the study of this animal behavior, experiments were performed with juvenile (7.57 ± 1.01, which were marked and kept in aquariums (30 l. To record activity, the aquariums were submitted to an artificial photoperiod (light and dark phases, 12h:12 h, with 16 shrimp (33 m² monitored in each phase for 20 days, for 15-minute periods every hour. Food was provided at random times and a record kept of horizontal and vertical displacement (continuous focal, swimming and exploration behavior (instantaneous focal. The horizontal and vertical displacements of the shrimp were greater during the dark phase, with a predominance of the swimming behavior. Exploration of the substrate occurred in both phases, with a peak between five and seven hours after the lights were turned on.

  10. Life on the beach for a sand crab (Emerita rathbunae (Decapoda, Hippidae: parasite-induced mortality of females in populations of the Pacific sand crab caused by Microphallus nicolli (Microphallidae

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    Juan Violante-González

    2016-09-01

    Full Text Available Parasites, by definition, can affect mortality of their host, making parasitism an important biotic determinant of animal population dynamics and community structure. Reduction in the number of larger, reproductive age females in populations of the Pacific sand crab, Emerita rathbunae (Decapoda, Hippidae, was observed in studies of the helminth community of this host. The aim of this study was to determine if high abundance of the metacercaria of the trematode, Microphallus nicolli (Microphallidae, causes mortality in this host. Females of E. rathbunae were collected from four sandy beaches in Guerrero State, Mexico, and helminths were collected from each crab. An analysis of variance (Anova was applied to these data in order to identify differences in abundance between sizes of crabs, and an analysis of covariance (Ancova was applied to identify differences in the abundance of metacercariae between locations. Parasite-related mortality was inferred by a decrease in abundance in older hosts. Linear and polynomial regressions of mean abundance of helminths (log x+1 transformed data vs. cephalothorax length of crabs were significant for the four populations of E. rathbunae, indicating increased mortality of older, more heavily infected female crabs and resultant removal from the population. Encapsulation and melanization of cysts by crabs was observed, indicating that an immune response by crabs also killed a portion of the cysts from subsequent exposures. Mortality of hosts through behavioral modification favoring transmission of highly infected crabs was suggested as the driving force behind this process.

  11. (H. Milne Edwards) (Decapoda: Penaeidae)

    African Journals Online (AJOL)

    aghomotsegin

    2014-01-24

    Jan 24, 2014 ... The structure of vasa deferentia and spermatophores has been described in Parapenaeopsis stylifera. The male reproductive system consists of two symmetrical halves; each half bears testis, vas deferens and an ejaculatory duct. Each testis comprised of two to three short, broad and milky white lobes; vas.

  12. Variaciones en la condición nutricional de langostas Panulirus argus (Decapoda: Palinuridae en la región este del Golfo de Batabanó, Cuba Variations of the nutritional condition of lobsters Panulirus argus (Decapoda: Palinuridae in Eastern region of the Gulf of Batabanó, Cuba

    Directory of Open Access Journals (Sweden)

    Alexander Lopeztegui Castillo

    2012-03-01

    Full Text Available En Cuba, la región del Golfo de Batabanó es la más importante zona de pesca de langosta debido a que de ella se extrae anualmente alrededor del 70% de la captura nacional. Con el objetivo de determinar variaciones espacio-temporales en la condición nutricional (K de langostas Panulirus argus del Golfo de Batabanó, Cuba, se calculó dicha condición mediante una relación pesolargo (K=Pt/Lt, para lo que se utilizaron ejemplares de cuatro zonas (uno, dos, cuatro y cinco del este del golfo, capturados en los años 1981 y 2010. Se encontró que en todos los casos analizados la condición nutricional fue significativamente mayor en machos que en hembras. Se detectaron variaciones significativas entre zonas en 1981 y 2010. También entre años el estado nutricional de las langostas presenta variaciones significativas, de manera que en 1981 (2.34±0.84g/mm fue mayor que en 2010 (1.96±0.49g/mm. Tanto las variaciones interzonales como las interanuales parecen estar relacionadas con variaciones reportadas en el tipo de fondo y la cobertura vegetal. Variaciones estacionales en la abundancia y distribución de organismos bentónicos que constituyen alimento para las langostas también podrían estar influyendo. Las diferencias entre sexos en cambio, se suponen consecuencia de la metodología utilizada y del dimorfismo sexual característico de esta especie, por cuanto otros métodos de estimación de K que no involucran mediciones morfométricas, no detectan estas diferencias.Variations of the nutritional condition of lobsters Panulirus argus (Decapoda: Palinuridae in Eastern region of the Gulf of Batabanó, Cuba. Nutritional condition can affect survival and growth rate of crustaceans, and this is mostly affected by habitat conditions. This study describes the space-temporary nutritional changes in this commercially important species. With this aim, the variations in the nutritional condition (K of lobsters from four zones (1, 2, 4 and 5 in the Gulf

  13. Efecto de la temperatura superficial y la salinidad en el reclutamiento del camarón rosado Farfantepenaeus duorarum (Decapoda: Penaeidae, en la Sonda de Campeche, Golfo de México

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    Mauricio Ramírez-Rodríguez

    2006-12-01

    Full Text Available Con el objetivo de evaluar los efectos a largo plazo de variables ambientales en la población de camarón rosado Farfantepenaeus duorarum en la Sonda de Campeche, al sur del Golfo de México, se analizaron los patrones de variación del reclutamiento, la salinidad y la temperatura superficial de 1969 a 1991. El reclutamiento muestra una clara tendencia decreciente desde la década de los años setenta, con una disminución acentuada desde los años ochenta. La temperatura superficial muestra una tendencia al aumento, particularmente desde 1972. La diferencia entre la temperatura a mediados de los setenta y finales de los ochenta fue de 0.5 °C. A lo largo del periodo de estudio la salinidad disminuyó. En el largo plazo, el reclutamiento muestra correlación negativa con la temperatura superficial y positiva con la salinidad. Los efectos de estas variables son estadísticamente significativos, explicando respectivamente el 52 % y 55 % de la variación del reclutamiento y, aunque no implican relaciones causa-efecto, sugieren simultaneidad de cambioImpact of surface temperature and salinity on the recruiting of the pink shrimp Farfantepenaeus duorarum (Decapoda: Penaeidae, in Sonda de Campeche, Gulf of Mexico. We studied the long term effects of two environmental variables, salinity and surface temperature, on the pink shrimp (Farfantepenaeus duorarum population in the southern Gulf of Mexico, considering the relationship between recruiting and the concurrent shrimp stock depletion of the last two decades. Our data were collected from 1969 to 1991. Recruitment has been clearly declining, particularly in the 1970s, with an accentuated drop since the 1980s. Sea surface temperatures have steadily risen, particularly since 1972. The temperature difference between the mid 1970s and the late 1980s is 0.5 °C. Salinity decreased throughout the period. From a long term perspective, recruitment is negatively correlated with temperature and positively

  14. İskenderun Körfezi'nde Dağılım Gösteren Kum Yengeci [Portunus pelagicus (Linneaus, 1758] ve Mavi Yengeç (Callinectes sapidus Rathbun, 1896 Populasyonları ve Morfometrisi Üzerinde Araştırmalar.

    Directory of Open Access Journals (Sweden)

    Tahir Özcan

    2015-12-01

    Full Text Available Bu çalışma ile İskenderun Körfezi’nden Temmuz 2002- Haziran 2003 tarihleri arsında elde edilen örnekler incelenerek Atlantik kökenli mavi yengeç ( Callinectes sapidus Rathbun, 1896 ve İndo-Pasifik kökenli kum yengeci (Portunus pelagicus Linneaus, 1758’nin boy dağılımları, erkek-dişi oranları belirlenmiştir. Toplam 467 birey (139 adet mavi yengeç ve 328 adet kum yengeci incelenmiştir. İskenderun Körfezi’ndeki kum yengeci populasyonunda %58 ile erkeklerin baskın olduğu, dişi bireylerin ortalama uzun karapas genişlikleri 119,9±2,8 mm genişliğinde, erkek bireylerin ortalama 104,9±2,1 mm uzun karapas genişliğinde oldukları belirlenmiştir. Mavi yengeç populasyonunda ise yaklaşık olarak populasyonun %69’unu dişi bireylerin oluşturduğu ve ortalama uzun karapas genişliği 148,9±2,5 mm, %31’ini oluşturan erkek bireylerin ise ortalama 123,3±3,4 mm uzun karapas genişliğinde oldukları belirlenmiştir

  15. Coeficientes de utilización digestiva aparente de materia seca, proteína y aminoácidos esenciales de ingredientes terrestres para el camarón del Pacífico Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Martín Terrazas

    2010-12-01

    Full Text Available Los aminoácidos esenciales y su disponibilidad son importantes en la formulación de alimentos. La digestibilidad de materia seca (DAMS, proteína (DAP y aminoácidos esenciales (DAAA fueron determinados (triplicado para el camarón blanco del Pacífico, Litopenaeus vannamei (15-19g, usando una dieta de referencia con 30% (de cada ingrediente. Los ingredientes evaluados fueron: caseína (CAS, harinas de subproductos avícolas (HSPA y porcícolas (HSPP, gluten de maíz (GLM y trigo (GLT, pasta de soya (PS, harinas de sorgo (HS y trigo (HT. La DAMS y DAP variaron entre 68%-109% y 70%-103%, respectivamente. La DAP en CAS, PS, HT y GLT fue mayor al 90%, en GLM y HSPA superior a 80%; HSPP (76% y HS (70% tuvieron menor digestibilidad. Hubo concordancia entre DAP y DAAA, excepto para Arg en GLM, Fen y Leu en HS, Fen en PS y Lis en HT y HSPA. Se encontró una gran variabilidad en la DAMS, DAP y DAAA en los ingredientes, lo que debe ser tomado en cuenta al formular alimentos para camarón.Apparent digestion coefficients for dry matter, protein and essential amino acids in terrestrial ingredients for Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae. Protein quality mainly depends on the essential amino acid (EAA profile, but also on its bioavailability, because EAA digestibility is generally lower than the analyzed amounts. This information is needed in the aquaculture industry for aquafeed formulation. For this purpose, the apparent digestibility coefficients of dry matter, protein, and essential amino acids of eight feedstuffs of terrestrial origin were determined for the juvenile whiteleg shrimp Litopenaeus vannamei (15-19g, using 1% chromic oxide as an inert marker. A reference diet was formulated and produced in the laboratory. Eight experimental diets were prepared each with 30% of one of the experimental ingredients added to the reference diet: casein, porcine byproduct meal poultry byproduct meal, corn meal, wheat gluten meal, soybean

  16. Zoological notes from port Dickson : III. Crustacea Anomura and Brachyura

    NARCIS (Netherlands)

    Buitendijk, A.M.

    1947-01-01

    From his stay at Port Dickson on the Malay Peninsula at the beginning of 1946 Major Dr. L. D. Brongersma of the N I C A Detachment brought home several species of crabs. A list of this material which is now incorporated in the collections of the Museum of Natural History at Leiden is given below,

  17. Activity and feeding of Dotilla fenestrata (Brachyura: Ocypodidae) in ...

    African Journals Online (AJOL)

    The activity of and consumption of organic material by the sand-bubbler crab Dotilla fenestrata was studied over neap and spring tides on a sheltered sand bank close to the mouth of the warm, temperate Kowie Estuary, South Africa. Crabs emerged from their burrows only after the tide receded, and it was light. Time to ...

  18. Embriologia do siri Arenaeus cribrarius (Lamarck (Crustacea, Brachyura, Portunidae Embryology of the swimming crab Arenaeus cribrarius (Lamarck (Crustacea, Brachyura, Portunidae

    Directory of Open Access Journals (Sweden)

    Marcelo A.A. Pinheiro

    2002-06-01

    Full Text Available Ovigerous females of Arenaeus cribrarius were collected at Ubatuba, São Paulo, and maintaining in aquaria under controlled temperature (25±1ºC, salinity (35±1‰ and photoperiod (12:12. A small sample of eggs was collected for each 24h, for morphological description, chromatic changes and biometric analysis throughout the embryonic development. Eight egg stages were photographed and had their small and large diameters measured under a microscope provided with camera lucida. The egg's shape was low elliptical during all the embryogenesis and showed size increase (24.4% and volume (91.1 %, when the last stage were compared with the first one. According to mean egg diameter and volume, the eggs should be pooled in three different groups (1-3; 4-6; 7-8 corresponding to initial, intermediate and final stages. The attained results were compared with the literature which emphasizing the species of Portunidae's family.

  19. Macroepizoísmo em Libinia ferreirae (Crustacea, Brachyura, Majidae Macroepizoites on Libinia ferreirae (Crustacea, Brachyura, Majidae

    Directory of Open Access Journals (Sweden)

    Vanessa C. Winter

    2006-06-01

    Full Text Available Um estudo de distribuição dos macroepizóicos foi realizado numa população do caranguejo-aranha Libinia ferreirae Brito Capello, 1871 proveniente do litoral dos estados do Paraná e de Santa Catarina. O material biológico foi obtido junto aos pescadores, e faz parte do rejeito de pesca. Os caranguejos foram mensurados e os macroepizóicos identificados e contados. Os seguintes macroepizóicos sésseis foram registrados: Calliactis tricolor (Lesueur, 1817 (Cnidaria; Actiniaria (Cnidaria; Arca sp. (Mollusca; Ostreidae (Mollusca; Acanthodesia tenuis (Desor, 1848 (Bryozoa; Cirripedia e duas espécies tubícolas de Gammaridea (Crustacea. Além destes organismos ocorreram dois tubos desabitados e quatro animais vágeis. A anêmona C. tricolor foi a espécie mais abundante e freqüente, sendo, provavelmente, utilizada como mecanismo de camuflagem pelo caranguejo. O macroepizoísmo em L. ferreirae está relacionado com a idade ou tamanho do caranguejo, tendo maior incidência naqueles mais velhos ou de maior porte. Entretanto, a densidade dos macroepizóicos por caranguejo se mantém em torno de três. Não há relação entre o macroepizoísmo e o sexo do hospedeiro; somente fêmeas ovígeras utilizam desse recurso mais freqüentemente do que as não-ovígeras. Devido à maior área de fixação, os macroepizóicos colonizam principalmente a carapaça do caranguejo, enquanto nos pereiópodos há predominância de Bryozoa.A study of the distribution of the macroepizoites was carried out on a population of the spider crab Libinia ferreirae Brito Capello, 1871 from the Southern Brazilian coast. Crabs were obtained from shrimps and fishes by-catch. They were measured, and their macroepizoites were identified and counted. The following sessile macroepizoites were registered: Calliactis tricolor (Lesueur, 1817 (Cnidaria; Actiniaria (Cnidaria; Arca sp. (Mollusca; Ostreidae (Mollusca; Acanthodesia tenuis (Desor, 1848 (Bryozoa; Cirripedia and two species of Gammaridea (Crustacea. The sea anemone C. tricolor was the most frequent species and probably it is used as a mechanism of camouflage by crabs. Macroepizoites on L. ferreirae are related to its age or size, therefore, bigger or older crabs had greater incidence of macroepizoites. However, the density of macroepizoites is about three per crab. There is not a relationship between macroepizoism and sex of the host, but ovigerous females use this resource more frequently than non-ovigerous ones. Due to larger setting area, macroepizoites colonize mainly the carapace of the host; bryozoans predominate in hosts' pereiopods.

  20. The complete mitochondrial genome sequence of Euphausia pacifica (Malacostraca: Euphausiacea) reveals a novel gene order and unusual tandem repeats.

    Science.gov (United States)

    Shen, Xin; Wang, Haiqing; Wang, Minxiao; Liu, Bin

    2011-11-01

    Euphausiid krill are dominant organisms in the zooplankton population and play a central role in marine ecosystems. Euphausia pacifica (Malacostraca: Euphausiacea) is one of the most important and dominant crustaceans in the North Pacific Ocean. In this paper, we described the gene content, organization, and codon usage of the E. pacifica mitochondrial genome. The mitochondrial genome of E. pacifica is 16 898 bp in length and contains a standard set of 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes. Translocation of three transfer RNAs (trnL(1), trnL(2), and trnW) was found in the E. pacifica mitochondrial genome when comparing with the pancrustacean ground pattern. The rate of K(a)/K(s) in 13 protein-coding genes among three krill is much less than 1, which indicates a strong purifying selection within this group. The largest noncoding region in the E. pacifica mitochondrial genome contains one section with tandem repeats (4.7 x 154 bp), which are the largest tandem repeats found in malacostracan mitochondrial genomes so far. All analyses based on nucleotide and amino acid data strongly support the monophyly of Stomatopoda, Penaeidae, Caridea, Brachyura, and Euphausiacea. The Bayesian analysis of nucleotide and amino acid datasets strongly supports the close relationship between Euphausiacea and Decapoda, which confirms traditional findings. The maximum likelihood analysis based on amino acid data strongly supports the close relationship between Euphausiacea and Penaeidae, which destroys the monophyly of Decapoda.

  1. Decapod larvae dynamics on Berlengas Biosphere Reserve (UNESCO - Portugal

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    Lénia Da Fonseca Alexandre Rato

    2014-05-01

    Total decapoda abundance ranged from 0,06 ind.m-3 in May 2011 to 64,28ind.m-3 in August 2012, and significantly different between summer/winter and winter/spring months (P(perm≤0,05. The data obtained on this study revealed that Infraorders Brachyura, Anomura and Caridea are the most common. All three are significantly different between months (P(perm≤0,05 but not between sampling stations (P(perm>0,05. Brachyuran abundance was significantly affected by the Oceanograhic Conditions (P(perm≤0,05. Abundances were higher in spring and summer months, when Chlorophyl a values (mg.m-3, Temperature (ºC and Salinity (ppt were also higher. Decapoda community is directly affected by the surrounding environmental conditions in Berlengas Biosphere Reserve and abundance might also be related with specific larvae release throughout the year. Each sampling station was considered a replica from the study area. The ecological importance of Berlengas was also verified by the presence of non-frequent larvae of Achelata and Stomatopoda.

  2. Análise comparativa da alimentação de peixes (Teleostei entre ambientes de marisma e de manguezal num estuário do sul do Brasil (Baía de Guaratuba, Paraná Study on feeding habits in estuarine fish (Teleostei comparatively between salt marshes and mangroves in southern Brazil (Guaratuba Bay

    Directory of Open Access Journals (Sweden)

    Paulo de T. Chaves

    2008-03-01

    Full Text Available Estudou-se a composição da dieta de peixes em dois tipos de ambiente de áreas rasas estuarinas - marisma e manguezal, objetivando avaliar se essas formações vegetais desencadeiam na ictiofauna respostas diferentes quanto à alimentação. As seis espécies avaliadas, as mais abundantes nessas áreas, mostraram-se predominantemente planctívoras, porém com particularidades quanto ao tipo de vegetação ocupada. Na marisma, Atherinella brasiliensis (Quoy & Gaimard, 1825 apresentou a dieta com maior número de itens e menor similaridade em relação às demais espécies. No manguezal tal isolamento coube a Anchoa januaria (Steindachner, 1879, espécie com maior participação de Decapoda Brachyura e Decapoda não-Brachyura, e única que nesse ambiente incluiu Gammaridae na dieta. Anchoviella lepidentostole (Fowler, 1911 identificou-se com Anchoa lyolepis (Evermann & Marsh, 1900 na marisma e com Opisthonema oglinum (Le Sueur, 1818 e Harengula clupeola (Cuvier, 1829 no manguezal. Uma situação comum a marisma e manguezal registrou-se entre O. oglinum e H. clupeola, espécies com dietas praticamente restritas a Diatomacea e Copepoda. Evidenciou-se o quanto as espécies são capazes de variar sua dieta com o ambiente, provavelmente em resposta à disponibilidade local. Mais que isso, porém, constatou-se que, seja na marisma, seja no manguezal, mesmo havendo mudança nos hábitos tróficos das espécies, cada uma delas mantém um padrão de diferenças em relação às demais que compõem a assembléia, fato que possivelmente assegura a abundância e coexistência entre elas nas áreas estuarinas rasas.Diet of fish inhabiting shallow waters close to salt marshes and mangroves was analyzed in order to evaluate how different the influence of these environments on fish feeding habits is. The six studied species, the most abundant in these areas, are mainly planctyvores, however they showed particularities in each area. In salt marshes Atherinella

  3. (Ortmann) (Decapoda, Crustacea) within the Swartkops estuary

    African Journals Online (AJOL)

    1987-03-13

    Mar 13, 1987 ... between the bivalve Macoma balthica and bacteria in intertidal sediments, Minas Basin, Bay of Fundy. J. Mar. Res. 35(3): 499-508. VAN DRIEL, C.D. 1978. Aspekte van die biologie van Pyura sto!onifera (Heller) (Tunicata: Ascidiacea) in Algoabaai met spesiale verwysing na die energie metabolisme. Ph.D.

  4. (Smith) (Decapoda: Parastacidae), on filter feeding

    African Journals Online (AJOL)

    One must therefore add to the list of factors that contribute to juvenile mortality the factors of food type and particle size. Although adult marron can presently be maintained for long periods of time in the laboratory, maintenance of the juvenile remains a problem. A diet for juveniles is presently being sought which will contain ...

  5. Crustacea Decapoda von der Insel Rhodos, Griechenland

    NARCIS (Netherlands)

    Lewinsohn, Ch.

    1976-01-01

    Obwohl uns schon Aristoteles (384-322 v. Chr.) über Decapoden aus dem Ägäischen Meer berichtet (Palinurus elephas (Fabricius) und Homarus gammarus (L.)), ist die Decapodenfauna dieser Region des östlichen Mittelmeeres bis heute nicht eingehend erforscht worden. Es gibt bis jetzt keine

  6. (Smith) (Decapoda: Parastacidae), on filter feeding

    African Journals Online (AJOL)

    the 'marron') introduced to South Africa from. Australia, are outlined. The high juvenile mortality rate problem is discussed. With reference to the morphology of the mouthparts of the juveniles, a type of diet is proposed which, it is suggested,.

  7. Use of blue crab (Callinectes sapidus) embryos for toxicity testing

    Energy Technology Data Exchange (ETDEWEB)

    Lee, R.; O`Malley, K. [Skidaway Inst. of Oceanography, Savannah, GA (United States)

    1995-12-31

    After fertilization, blue crab embryos develop in egg sacs attached to the female pleopods, often referred to as the sponge. Lipovitellin and lipid droplets in the egg sacs provide energy and nutrition for the developing embryos. Embryos were removed from the sponge and transferred to 24 well culture plates containing sea water with or without toxicants, Each well contained 10 embryos. After 7 to 10 days, embryos hatched to swimming zoea. The effects of toxicants at various concentrations on hatching were determined and the EC{sub 50} calculated. For example, the EC{sub 50} for tributyltin, fenvalerate and mercuric chloride were 50, 30 and 90 ng/liter, respectively. The hatching success of control embryos ranged from 95 to 98%. Formation of the heart, eyespot formation, appendage formation and utilization rate of lipovitellin were also effected by exposure to toxicants. At a low concentration of mercuric ion (30ng/liter) the heart formed, but there was no heart beat. Eyespot formation was abnormal in the presence of high concentrations of cadmium (2 {micro}g/liter) and zinc (5 {micro}g/liter), Crab embryos offer many advantages for toxicity testing of pure compounds or mixtures in water, including toxicity testing of sediment pore water. The crab embryos may also serve as models to understand the effect of specific toxicants on the heart and eye spots of crustaceans.

  8. Feeding of guitarfish Rhinobatos percellens (Walbaum, 1972 (Elasmobranchii, Rhinobatidae, the target of artisanal fishery in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Hugo Bornatowski

    2010-03-01

    Full Text Available Rhinobatos percellens is one of three species of Rhinobatidae found on Brazilian shores and is one of the most abundant species on the shallow continental shelf of Paraná and Santa Catarina States. Specimens caught by artisanal fishery between July/2001 and March/2003 by fishing communities located on the frontier between two southern Brazilian States (Paraná and Santa Catarina had their stomach contents analyzed. According to the Alimentary Index (IAi, Decapoda (69% and Teleostei (22% were the main items consumed. Polychaeta, a common prey consumed by several benthonic fishes, was poorly represented in the feeding of R. percellens. This fact may be related to the availability of prey in the environment, or to the size of the guitarfish analyzed (ontogeny. Seasonal variance of main preys (with higher IAi was observed: Dendrobranchiata and Pleocyemata displayed higher percentages in spring and autumn, Teleostei in the winter and Brachyura during the summer.Rhinobatos percellens é uma das três espécies de Rhinobatidae encontrada na costa brasileira e uma das espécies de elasmobrânquio mais abundante na plataforma continental dos estados do Paraná e Santa Catarina. Indivíduos capturados pela pesca artesanal entre Julho/2001 e Março/2003 nas comunidades pesqueiras localizadas entre o Paraná e Santa Catarina tiveram seus conteúdos estomacais analisados. O Índice de Importância Alimentar (IAi mostrou que as principais presas consumidas foram Decapoda (69% e Teleostei (22%. Polychaeta, principal presa ingerida por várias espécies de peixes bentônicos foi pouco expressiva na alimentação de R. percellens. Essa diferença pode estar relacionada com a disponibilidade de presas no ambiente ou com o tamanho das raias analisadas (ontogenia. De acordo com análises sazonais, observou-se que os camarões Dendrobranchiata e Pleocyemata foram as principais presas consumidas durante a primavera e outono, Teleostei durante o inverno e Brachyura

  9. Genetic analyses determine connectivity among cave and surface populations of the Jamaican endemic freshwater crab Sesarma fossarum in the Cockpit Country

    Directory of Open Access Journals (Sweden)

    Manuel Stemmer

    2016-01-01

    Full Text Available The Jamaican freshwater crab Sesarma fossarum (Decapoda: Brachyura: Sesarmidae is endemic to western central Jamaica where it occurs in cave and surface streams of karst regions. In the present study, we examine the population genetic structure of the species, providing evidence for intraspecific differentiation and genetic substructure among twelve sampled populations. Interestingly, crabs from caves appear genetically undistinguishable from representatives of nearby surface waters, despite previously observed and described morphometric differentiation. In contrast, genetic isolation takes place among populations from rivers and caves belonging to different watersheds. In one case, even populations from different tributaries of the same river were characterized by different genotypes. Overall, the species shows low haplotype and nucleotide diversities, which indicates a high homogeneity and point towards a relatively recent intraspecific radiation and diversification. Our results on the genetic diversification of S. fossarum helps to reconstruct unknown subterranean water flow and cave connections in its native range, allowing prediction of its further dispersal and differentiation potential. Unfortunately, its natural habitat of Jamaican cockpit karst, which also is home to several other endemic species and is a globally-recognized Key Biodiversity Area, is under imminent threat of intensive bauxite mining.

  10. Spatial and temporal distribution of decapod larvae in the subtropical waters of the Arvoredo archipelago, SC, Brazil Distribuição espacial e temporal de larvas de decápodos nas águas subtropicais do arquipélago do Arvoredo, SC, Brasil

    Directory of Open Access Journals (Sweden)

    Andréa G. Koettker

    2006-03-01

    Full Text Available The present paper aims to describe the temporal and spatial distribution of the composition and abundance of Decapoda larvae in the shallow waters around Arvoredo Marine Biological Reserve. Stomatopod occurrence is also discussed. Plankton samples were collected at five sites around the Arvoredo Island every two months for one year from May, 2002 to April, 2003. Thirty-nine morphotypes, 11 genus and 4 species (Artemesia longinaris Bate, 1888, Hexapanopeus schmitii Rathbun, 1930, Menippe nodifrons Stimpson, 1859 and Pleoticus muelleri Bate, 1888 were identified, among them only two morphotypes of Stomatopoda larvae, and the remainder Decapoda larvae. Brachyuran zoeae were the most abundant group and they were well represented by Portunidae and Xanthidae zoeae. Lucifer sp. and Caridea zoeae were the most abundant non-brachyuran taxa. Decapod larvae were observed to occur at all sampling sites, however the spatial distribution demonstrated a general tendency to greater abundance and diversity at the southern sites of the Island. Decapoda and Stomatopoda larvae occurred throughout the year, showing that reproduction is continuous, but that larval input in planktonic community was significantly higher during autumn and spring.Este trabalho tem como objetivo a descrição da distribuição temporal e espacial da composição e abundância de larvas de decápodes nas águas rasas ao redor da Reserva Biológica Marinha do Arvoredo. A ocorrência de larvas de estomatópodes também foi discutida. Foram realizadas coletas de plâncton em cinco estações ao redor da Ilha do Arvoredo, de maio de 2002 a abril de 2003 a cada dois meses. Foram identificados 39 morfotipos, 11 gêneros e 4 espécies (Artemesia longinaris Bate, 1888, Hexapanopeus schmitii Rathbun, 1930, Menippe nodifrons Stimpson, 1859 and Pleoticus muelleri Bate, 1888, sendo apenas dois morfotipos da Ordem Stomatopoda e o restante da Ordem Decapoda. Zoés de Brachyura foi o grupo mais abundante

  11. Prediction of Scylla olivacea (Crustacea; Brachyura) peptide hormones using publicly accessible transcriptome shotgun assembly (TSA) sequences.

    Science.gov (United States)

    Christie, Andrew E

    2016-05-01

    The aquaculture of crabs from the genus Scylla is of increasing economic importance for many Southeast Asian countries. Expansion of Scylla farming has led to increased efforts to understand the physiology and behavior of these crabs, and as such, there are growing molecular resources for them. Here, publicly accessible Scylla olivacea transcriptomic data were mined for putative peptide-encoding transcripts; the proteins deduced from the identified sequences were then used to predict the structures of mature peptide hormones. Forty-nine pre/preprohormone-encoding transcripts were identified, allowing for the prediction of 187 distinct mature peptides. The identified peptides included isoforms of adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin B, allatostatin C, bursicon β, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone/molt-inhibiting hormone, diuretic hormone 31, eclosion hormone, FMRFamide-like peptide, HIGSLYRamide, insulin-like peptide, intocin, leucokinin, myosuppressin, neuroparsin, neuropeptide F, orcokinin, pigment dispersing hormone, pyrokinin, red pigment concentrating hormone, RYamide, short neuropeptide F, SIFamide and tachykinin-related peptide, all well-known neuropeptide families. Surprisingly, the tissue used to generate the transcriptome mined here is reported to be testis. Whether or not the testis samples had neural contamination is unknown. However, if the peptides are truly produced by this reproductive organ, it could have far reaching consequences for the study of crustacean endocrinology, particularly in the area of reproductive control. Regardless, this peptidome is the largest thus far predicted for any brachyuran (true crab) species, and will serve as a foundation for future studies of peptidergic control in members of the commercially important genus Scylla. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. Marine pollution effects on the southern surf crab Ovalipes trimaculatus (Crustacea: Brachyura: Polybiidae) in Patagonia Argentina.

    Science.gov (United States)

    Lezcano, Aníbal Hernán; Rojas Quiroga, María Laura; Liberoff, Ana Laura; Van der Molen, Silvina

    2015-02-28

    We compared the carapace shape and thickness as well as the energy density of Ovalipes trimaculatus inhabiting areas comprising a gradient of marine pollution: high, moderate and undetected, in the Nuevo gulf (Patagonia Argentina). The carapace shape was evaluated by means of individual asymmetry scores (=fluctuating asymmetry) whereas the carapace thickness was assessed by measuring the carapace dry weight. The energy density was analyzed through its negative relationship with water content in muscle tissue. The individual asymmetry scores as well as the percentage of water content in muscle tissue were proportional to the marine pollution gradient, whereas the carapaces thickness did not differ among sampling sites. Our results are consistent with previous findings and demonstrate the direct effect of marine pollution on other taxa different from gastropods, cephalopods and polyplacophora and add to long-standing concerns about detrimental effects caused by marine pollution on the benthic community of the Nuevo gulf. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. A new species of Euprognatha Stimpson, 1871 (Crustacea, Brachyura, Inachoididae from off coast of northeastern Brazil

    Directory of Open Access Journals (Sweden)

    William Santana

    2008-01-01

    Full Text Available A new species of Euprognatha Stimpson, 1871 from off coast of Brazil (Canopus Bank, 02º15.3'00"S - 38º16.0'00"W is described and illustrated, namely Euprognatha limatula n. sp. The new species is compared to its congeners. Lectotypes are designated for E. acuta A. Milne-Edwards, 1880 and E. granulata Faxon, 1893. A key to the species of Euprognatha is provided.Uma nova espécie do gênero Euprognatha Stimpson, 1871 coligida ao largo da costa brasileira (monte submarino Canopus, 02º15.3'00"S - 38º16.0'00"W é descrita e ilustrada, nomeadamente Euprognatha limatula n. sp. A nova espécie é comparada às suas congêneres. Lectótipos são designados para E. acuta A. Milne-Edwards, 1880 and E. granulata Faxon, 1893. É fornecida uma chave de identificação para as espécies de Euprognatha.

  14. Mass spectrometric characterization of the neuropeptidome of the ghost crab Ocypode ceratophthalma (Brachyura, Ocypodidae).

    Science.gov (United States)

    Hui, Limei; D'Andrea, Brandon T; Jia, Chenxi; Liang, Zhidan; Christie, Andrew E; Li, Lingjun

    2013-04-01

    The horn-eyed ghost crab Ocypode ceratophthalma is a terrestrial brachyuran native to the Indo-Pacific region, including the islands of Hawaii. Here, multiple mass spectrometric platforms, including matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) and nanoflow liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (nanoLC-ESI-Q-TOF MS/MS), were used to characterize the neuropeptidome of this species. In total, 156 peptide paracrines/hormones, representing 15 peptide families, were identified from the O. ceratophthalma supraesophageal ganglion (brain), eyestalk ganglia, pericardial organ and/or sinus gland, including 59 neuropeptides de novo sequenced here for the first time. Among the de novo sequenced peptides were isoforms of A-type allatostatin, B-type allatostatin, FMRFamide-like peptide (FLP), orcokinin, orcomyotropin and RYamide. Of particular note, were several novel FLPs including DVRAPALRLRFamide, an isoform of short neuropeptide F, and NRSNLRFamide, the orcokinins NFDEIDRSGYGFV and DFDEIDRSSFGFH, which exhibit novel Y for F and D for N substitutions at positions 10 and 1, respectively, and FDAYTTGFGHS, a member of the orcomyotropin family exhibiting a novel Y for F substitution at position 4. Taken collectively, the set of peptides described here represents the largest number of neuropeptides thus far characterized via mass spectrometry from any single crustacean, and provides a framework for future investigations of the physiological roles played by these molecules in this species. Copyright © 2013 Elsevier Inc. All rights reserved.

  15. Mass spectrometric characterization of the neuropeptidome of the ghost crab Ocypode ceratophthalma (Brachyura, Ocypodidae)

    OpenAIRE

    Hui, Limei; D’Andrea, Brandon T.; Jia, Chenxi; Liang, Zhidan; Christie, Andrew E.; Li, Lingun

    2013-01-01

    The horn-eyed ghost crab Ocypode ceratophthalma is a terrestrial brachyuran native to the Indo-Pacific region, including the islands of Hawaii. Here, multiple mass spectrometric platforms, including matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS/MS) and nanoflow liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (nanoLC-ESI-Q-TOF MS/MS), were used to characterize th...

  16. Development of the male reproductive system in Callinectes ornatus Ordway, 1863 (Brachyura: Portunidae

    Directory of Open Access Journals (Sweden)

    Fabiana Aparecida do Nascimento

    Full Text Available This study describes the histology and histochemistry of the male reproductive system in Callinectes ornatus, comparing juvenile and adult developmental stages. We also analyzed changes in the gonadosomatic (GSI and hepatosomatic (HSI indices, and the weights of the testis and vas deferens during the development. The results showed that all stages, beginning with the juvenile (JUV, through developing (DEV and mature (MAT adult males of C. ornatus produce sperm and spermatophores. During development, testicular lobes showed the same characteristics of production and release of sperm into the seminiferous duct. The vas deferens showed little histological and histochemical change in the epithelium in juvenile and adult males. The differences consisted of the larger amount of secretion in MAT males compared to JUV and DEV ones. The chemical composition of the seminal fluid was similar, but MAT males produced a more homogeneous secretion. Morphological and physiological maturation are not synchronized in C. ornatus, since JUV males produced spermatophores similar to those in DEV and MAT males. However, these JUV are not yet able to reproduce, since they still have the abdomen attached to the cephalothoracic sternum. The increase of the GSI during development was significant for MAT males, and is related to the production of sufficient volume of seminal fluid to form the sperm "plug" in the female seminal receptacle. The HSI decreased from DEV to MAT adult stages, indicating that reserves from the hepatopancreas are used to develop the reproductive system after the pubertal molt.

  17. Reproductive investment and multiple spawning evidence in the redfinger rubble crab Eriphia gonagra (Brachyura, Eriphioidea

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    Gustavo M. Teixeira

    Full Text Available Abstract The variation in reproductive investment (RI and the hypothesis of multiple spawning were evaluated in the redfinger rubble crab Eriphia gonagra (Fabricius, 1781. The gonads and embryos showed synchronous development, and fecundity and RI varied widely among females of the same size class. The mean RI value recorded was 11.31%, with no significant differences among the means for different size classes. The allometric analysis of fecundity indicated RI decrease while body size increase, but we suggested that this occurs due to size overestimation where the largest width of carapace was used as body size reference in these analyzes. In addition, we found an isometric relationship for “female weight vs. egg number”, and also for “female weight vs. egg weight”, indicating that RI increased proportionally with size of females. Relatively high frequencies both of smaller females with rudimentary gonads, and of larger females with developed gonads were observed. This indicates that larger females take place more frequently in the population reproductive output over time. This difference could not be observed by means of RI analyses of captured and fixed crabs, for which only one stage of gonad development and/or one spawning is usually recorded.

  18. Female reproductive cycle of the Southwestern Atlantic estuarine crab Chasmagnathus granulatus (Brachyura, Grapsoidea, Varunidae

    Directory of Open Access Journals (Sweden)

    Romina B. Ituarte

    2004-03-01

    Full Text Available The female reproductive biology of a Chasmagnathus granulatus population inhabiting the area near the mouth of Mar Chiquita coastal lagoon, Argentina, was studied. An increase in air temperature during the spring is related to the start of the breeding period, when well defined egg-laying and hatching pulses were observed. Hatching is synchronic during the whole summer but the egg production was not, probably due to the gradual incorporation of young females to the reproductive population. Neither egg-laying nor larval release showed a clear relation to moon phase or tidal cycles, suggesting that reproduction is not rigidly programmed in this unpredictable habitat. Females moult at the beginning of autumn, after releasing the last larvae. However, a new cohort of ovocytes, which was in primary vitellogenesis before moulting, completed the secondary ovogenesis after moulting. Consequently, ovaries remained fully developed throughout the winter.

  19. The first zoeal stage of Parthenope macrochelos (Herbst, 1790 hatched in the laboratory (Crustacea: Brachyura: Parthenopidae

    Directory of Open Access Journals (Sweden)

    Guillermo Guerao

    1999-03-01

    Full Text Available The first zoeal stage of the parthenopid crab Parthenope macrochelos is described and illustrated from laboratory-hatched material obtained from an ovigerous crab captured in the western Mediterranean. The first larva of P. macrochelos possesses all the characters as diagnostic of the Parthenopidae and some additional features, including a seta on the basal segment of the endopod of the maxillule and a well developed lateral spine on the telson forks. The present stage is compared with those previously described from other species of the genus Parthenope.

  20. The fiddler crabs (Crustacea: Brachyura: Ocypodidae: genus Uca of the South Atlantic Ocean

    Directory of Open Access Journals (Sweden)

    Luis Ernesto Arruda Bezerra

    Full Text Available The taxonomy of the 11 species of fiddler crabs [Uca (Uca maracoani (Latreille, 1802-1803, U. (U. tangeri (Eydoux, 1835, U. (Minuca burgersi Holthuis, 1967, U. (M. mordax (Smith, 1870, U. (M. rapax (Smith, 1870, U. (M. thayeri Rathbun, 1900, U. (M. victoriana von Hagen, 1987, U. (M. vocator (Herbst, 1804, U. (Leptuca cumulanta Crane, 1943, U. (L. leptodactyla Rathbun, 1898 and U. (L. uruguayensis Nobili, 1901] of the South Atlantic Ocean is reviewed. Keys for identification, updating the keys for the Atlantic Ocean are proposed, including the species recently described. Comments reporting morphological variations among types and additional material and among populations of different localities are included. The Atlantic species are divided into three subgenera: Uca s. str., Minuca and Leptuca. The eastern Atlantic species U. (U.tangeri is included in subgenus Uca s. str. due to the presence of a proximal spine opposing the spoon-tipped setae of the second maxilliped, which is considered an apomorphic character of the subgenus Uca s. str.

  1. Population structure of the burrowing crab Neohelice granulata (Brachyura, Varunidae in a southwestern Atlantic salt marsh

    Directory of Open Access Journals (Sweden)

    Sabrina Angeletti

    2015-07-01

    Full Text Available Neohelice granulata inhabits estuarine and protected coastal areas in temperate regions and is the most dominant decapod crustacean in the Bahía Blanca Estuary, Argentina. The population structure was studied during a year in a SW Atlantic salt marsh located in the Bahía Blanca Estuary. Crabs were sampled monthly from August 2010 to July 2011. The maximum observed density was 30 crabs m-2 in February and 70 burrows m-2 in May. The maximum carapace width (CW was 32 and 27.5 mm in males and females respectively. Medium size crabs were between 16 and 20 mm CW. Significantly smaller sized crabs were observed at the lower intertidal regions (P < 0.05. The sex ratio was favorable for males and was significantly different from the expected 1:1 (P < 0.05. The recruitment of unsexed juveniles crabs (CW <6.5 mm was observed throughout the year and the presence of ovigerous females from October to February indicated seasonal reproduction. The average size of ovigerous females was CW = 20.8 mm and the smallest ovigerous female measured was 16 mm CW. For the first time, the population structure of the most important macro-invertebrate is analyzed in the Bahía Blanca Estuary. This study may help to make decisions in the area, where anthropic action is progressing day by day.

  2. Tallas y fecundidad de Juxtafabia muliniarum (Brachyura: Pinnotheridae asociado con Saccostrea palmula (Bivalvia: Ostreidae, Costa Rica

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    Jorge Cabrera Peña

    2001-12-01

    Full Text Available Se determinaron las tallas, frecuencia de infección y fecundidad de Juxtafabia muliniarum de la cavidad paleal de Saccostrea palmula de mayo de 1998 a mayo de 1999, la frecuencia de infestación fue de un 18.52 % en una muestra de 540 ostras. De los 136 cangrejos, el 36 % fueron parejas, el 60 % hembras solas y el 4 % machos solos. El promedio de la longitud del caparazón fue de 5.6 + 0.74 mm (ámbito entre 4.0 y 7.6 mm para hembras y 2.71 + 0.60 mm (ámbito entre 1.6 y 4.0 mm para machos. El peso promedio fue de 0.180 + 0.084 g (ámbito entre 0.06 y 0.4 g para hembras y 0.011 + 0.003 g (ámbito entre 0.01 y 0.02 g para machos. La fecundidad de J. muliniarum se rigió por la ecuación F = 3904.6 Ln (Lc -4651.1. En todos los meses se encontraron hembras ovígeras. La relación longitud del caparazón-peso total para machos y hembras se rigió porla ecuación Pt = 6 X 10-4 Lc3.2122. La proporción sexual fue de 1.0 macho: 2.04 hembras. Se registra a S. palmula como huésped de J. muliniarumSize and fecundity observations of pea crab (Juxtafabia muliniarum from the paleal cavity of!he oyster Saccostrea palmula were made from May 1998 to May 1999. Infestation frequency was 18.52 % in a sample of 540 oysters. Of 136 pea crabs, 36 % were couples, 60 % were single females and 4 % were single males. The mean caparace length of J. muliniarum was 5.6 + 0.74 mm (range 4.0 to 7.6 mm for females and 2.7 + 0.60 mm (range 1.6 to 4.0 mm for males. The mean weight was 0.180 + 0.084 g (range 0.06 to 0.4g for females and 0.011 + 0.003 g (range 0.01 to 0.02 g for males. Ovigerous females (43.75 % of all females were found in all months. The caparace length - fecundity relationship was F = 3904.6 Ln (Lc -4651.1. The caparace length-weight relationship was P = 6 X 10-4 Lc3.2122. The mean sex-ratio was 1.0 male: 2.4 females. Saccostrea palmula infected only by females was the dominant group (60.78 %. This mollusk is a new host record for the crab

  3. On a new species of Uca from the West Indian region (Crustacea, Brachyura, Ocypodidae)

    NARCIS (Netherlands)

    Holthuis, L.B.

    1967-01-01

    A paper on the Ocypodid crabs of the Netherlands Antilles, which is now in preparation, was held up by the fact that one of the species of Uca, commonly found on practically all of the islands, proved to need a new name, which I expected to be introduced by Dr. Jocelyn Crane in her coming monograph

  4. Can contrasting environmental conditions of mangroves induce morphological variability in Aratus pisonii (Crustacea: Brachyura: Sesarmidae?

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    Beatriz López-Sánchez

    2016-09-01

    Full Text Available Aratus pisonii is one of the most common crab species in Neotropical mangroves. It shows great plasticity in its life history traits, which makes it an interesting subject for comparative studies. This study evaluated the morphometric variability in five populations of A. pisonii inhabiting mangroves with different degrees of structural development under contrasting environmental conditions. Mangrove forests located on the northwest coast of Venezuela were studied during the rainy season in 2006. The results showed morphometric differences and interaction between sampling sites and sex (PERMANOVA, P=0.0001, as well as the presence of five morphological groups in males and four in females. The findings support the existence of sexual dimorphism. Females from the dwarf hypersaline mangrove showed a wide variability associated with the chelipeds. The differences in crab morphology between sites seem to be related to a combination of environmental factors that is unique for each habitat, leading to the formation of different morphological groups, in which the mangrove structural development (resource availability and salinity (which compromises the energy budget play an important role. The presence of more robust chelipeds in females from the dwarf hypersaline mangrove seems to reflect an adaptation to the biomechanical properties of the leaves (sclerophylly.

  5. Phylogenetic patterns and the adaptive evolution of osmoregulation in fiddler crabs (Brachyura, Uca)

    Science.gov (United States)

    Faria, Samuel Coelho; Provete, Diogo Borges; Thurman, Carl Leo

    2017-01-01

    Salinity is the primary driver of osmoregulatory evolution in decapods, and may have influenced their diversification into different osmotic niches. In semi-terrestrial crabs, hyper-osmoregulatory ability favors sojourns into burrows and dilute media, and provides a safeguard against hemolymph dilution; hypo-osmoregulatory ability underlies emersion capability and a life more removed from water sources. However, most comparative studies have neglected the roles of the phylogenetic and environmental components of inter-specific physiological variation, hindering evaluation of phylogenetic patterns and the adaptive nature of osmoregulatory evolution. Semi-terrestrial fiddler crabs (Uca) inhabit fresh to hyper-saline waters, with species from the Americas occupying higher intertidal habitats than Indo-west Pacific species mainly found in the low intertidal zone. Here, we characterize numerous osmoregulatory traits in all ten fiddler crabs found along the Atlantic coast of Brazil, and we employ phylogenetic comparative methods using 24 species to test for: (i) similarities of osmoregulatory ability among closely related species; (ii) salinity as a driver of osmoregulatory evolution; (iii) correlation between salt uptake and secretion; and (iv) adaptive peaks in osmoregulatory ability in the high intertidal American lineages. Our findings reveal that osmoregulation in Uca exhibits strong phylogenetic patterns in salt uptake traits. Salinity does not correlate with hyper/hypo-regulatory abilities, but drives hemolymph osmolality at ambient salinities. Osmoregulatory traits have evolved towards three adaptive peaks, revealing a significant contribution of hyper/hypo-regulatory ability in the American clades. Thus, during the evolutionary history of fiddler crabs, salinity has driven some of the osmoregulatory transformations that underpin habitat diversification, although others are apparently constrained phylogenetically. PMID:28182764

  6. Checklist of fossil decapod crustaceans from tropical America. Part I: Anomura and Brachyura

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    Javier Luque

    2017-10-01

    Full Text Available Abstract Our knowledge of fossil crustaceans from the tropics has increased considerably during recent decades, thanks to novel findings and the re-examination of museum specimens. However, several previous records have been misidentified, numerous museum specimens have never been reported, and many new discoveries are yet to be published. Here, we present a detailed, up-to-date, and revised checklist for every marine, terrestrial, or freshwater fossil decapod crustacean occurrence from tropical America known to us, including their age, geographic occurrences, and related literature. We recognize the occurrence of at least 32 superfamilies, 69 families, 190 genera, and 415 species of brachyurans (‘true’ crabs, and anomurans (‘false’ crabs, hermit crabs, squat lobsters, and allies, several of them previously unknown. The checklist comprises records from three main geographic regions: 1 northern South America (Bolivia, Brazil, Chile, Colombia, Ecuador, Peru, Venezuela; 2 Central America and southern North America (Belize, Costa Rica, Honduras, Panama, Mexico, southern and central Florida; and 3 the Caribbean Islands + Bermuda (Anguilla, Antigua, Aruba, Bahamas, Barbados, Bermuda, Bonaire, Cuba, Curaçao, Dominican Republic, The Grenadines, Haiti, Jamaica, Puerto Rico, Saint Bartélemy, Saint Martin, Trinidad. Previous findings, new occurrences, and the revised systematic placement for several problematic/misidentified records, indicate that the fossil record of anomurans and brachyurans in tropical America is more diverse than previously envisioned, with a considerable degree of endemism at the genus- and species-levels.

  7. Post-larval development and sexual dimorphism of the spider crab Maja brachydactyla (Brachyura: Majidae

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    Guillermo Guerao

    2009-12-01

    Full Text Available The post-larval development of the majid crab Maja brachydactyla Balss, 1922 was studied using laboratory-reared larvae obtained from adult individuals collected in the NE Atlantic. The morphology of the first juvenile stage is described in detail, while the most relevant morphological changes and sexual differentiation are highlighted for subsequent juvenile stages, until juvenile 8. The characteristic carapace spines of the adult phase are present in the first juvenile stage, though with great differences in the degree of development and relative size. The carapace shows a high length/weight ratio, which becomes similar to that of adults at stage 7-8. Males and females can be distinguished from juvenile stage 4, based on sexual dimorphism in the pleopods and the presence of gonopores. In addition, the allometric growth of the pleon is sex-dependent from juvenile stage 4, with females showing a positive allometry (b=1.23 and males an isometric allometry (b=1.02.

  8. Life history of plesionika edwardsi (crustacea, decapoda, Pandalidae)

    African Journals Online (AJOL)

    The life history of Plesionika edwardsi (Brandt, 1851) around the Canary Islands in the Eastern Central Atlantic was investigated, based on a total of 11 434 shrimps ranging in length between 8 and 40 mm carapace length (CL). The species carries out seasonal migrations; they concentrate in deep water during winter, ...

  9. Spermatozoal ultrastructure in three Atlantic solenocerid shrimps (Decapoda, Dendrobranchiata).

    Science.gov (United States)

    Medina, Antonio; Scelzo, Marcelo A; Tudge, Christopher C

    2006-03-01

    The spermatozoal ultrastructure in three solenocerid shrimps (Solenocera membranacea, S. africana, and Pleoticus muelleri) from different Atlantic locations was examined with the aim of increasing understanding of the phylogenetic relationships in the Dendrobranchiata. A considerable structural similarity between the sperm of these species and those of penaeid shrimps supports a close affinity between the Penaeidae and Solenoceridae. However, significant differences in the sperm morphology of the previously investigated sicyoniids (namely, a greater complexity of the acrosomal complex) suggest evolutionary separation of the Sicyoniidae from the assemblage Penaeidae-Solenoceridae. Two ultrastructural features distinguish the spermatozoa of the three studied solenocerids from penaeid sperm: 1) separation of the plasma and acrosome membranes at the base of the spike and anterior region of the cap, and 2) asymmetry of the acrosomal cap, which appears to be a synapomorphy of the group. No striking ultrastructural differences were found between the spermatozoa of the closely related species S. membranacea and S. africana, whereas a great number of morphological differences separate the spermatozoa of Pleoticus from those of Solenocera (e.g., shape of the acrosomal cap, structural arrangement of the contents of the whole acrosome vesicle, thickness and distribution of the cytoplasm, and external shape of the spike).

  10. Current state of knowledge on Virilastacus species (Crustacea, Decapoda, Parastacidae

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    Erich H Rudolph

    2015-11-01

    Full Text Available The genus Virilastacus was created in 1991 to accommodate Parastacus araucanius Faxon, 1914. At present, Virilastacus comprises four burrowing species, three of which were described at the beginning of the XXI century, and biological knowledge about these species is mainly limited to taxonomic and distributional aspects. This review compiles published information about these species, together with other data available to the author in order to update the current state of biological knowledge and, in turn, to promote the conservation of these species. An upgraded diagnosis of the genus Virilastacus is provided, together with information related to each species with regard to: distinctive morphological traits, geographic distribution, habitat, burrow morphology, burrowing behavior, body size, sexual system, and state of conservation. Some aspects related to morphological adaptations to their burrowing life style, phylogenetic affinities and main threats to conservation are also discussed. It is concluded that biological knowledge about these four species is scarce and fragmentary; furthermore, they are currently under threat as a result of anthropogenic activities that are degrading and fragmenting their habitat.

  11. Lysmata seticaudata (Decapoda, Crustacea) als Putzergarnele im Mittelmeer

    Science.gov (United States)

    Patzner, R. A.

    1982-06-01

    Members of the shrimp species Lysmata seticaudata were observed to clean a morrey eel in the western Mediterranean Sea (Baleares, Spain). In contrast to the cleaning symbiosis of tropical shrimps, several Lysmata species have only one host, thus representing a primitive type of cleaning behaviour. Lysmata seticaudata is not dependent upon cleaning for food and usually these shrimps are found without a host.

  12. The abundance and composition of crabs (Decapoda) in Uta Ewa ...

    African Journals Online (AJOL)

    ... abundant accounting for 46.6% of the percentage abundance. Station 2 had the highest Shannon diversity index of 1.22, while Station 1 had 1.15. The abundance and composition of crab species in the study areas is an indication that the area is an important ecological zone for benthos macroinvertebrates conservation.

  13. Phylogenetic relationships within the snapping shrimp genus Synalpheus (Decapoda: Alpheidae).

    Science.gov (United States)

    Hultgren, Kristin M; Hurt, Carla; Anker, Arthur

    2014-08-01

    The snapping shrimp genus Synalpheus (Alpheidae) is one of the most speciose decapod genera, with over 160 described species worldwide. Most species live in symbiotic relationships with other marine organisms, such as sponges, corals and crinoids, and some sponge-dwelling species have a highly organized, social structure. The present study is the first worldwide molecular phylogenetic analysis of Synalpheus, based on >2,200 bp of sequence data from two mitochondrial (COI and 16S) and two nuclear (PEPCK and 18S) loci. Our molecular data show strong support for monophyly of three out of six traditionally recognized morphology-based species groups: the S. brevicarpus, S. comatularum and S. gambarelloides groups. The remaining three species groups (S. paulsoni, S. neomeris and S. coutierei groups) are non-monophyletic in their current composition and will need to be either abandoned or taxonomically redefined. We also identified potential cryptic species of Synalpheus in our dataset, using intraspecific and interspecific sequence variation in COI from the taxonomically well-studied S. gambarelloides group to establish a genetic divergence threshold. We then used both genetic divergence and tree-based criteria (reciprocal monophyly) to identify potential cryptic species in the remaining taxa of the genus. Our results suggest the presence of multiple cryptic lineages in Synalpheus, underlining the need for more integrative taxonomic studies-including morphological, ecological, molecular, and color pattern data-in this biologically interesting genus. Copyright © 2014 Elsevier Inc. All rights reserved.

  14. A new dicyemid from Sepiella japonica (Mollusca: Cephalopoda: Decapoda).

    Science.gov (United States)

    Furuya, Hidetaka

    2008-02-01

    A new species of dicyemid mesozoan is described from Sepiella japonica Sasaki, 1929, collected from the eastern area of Inland Sea, Osaka Bay, and Kii Strait in Japan. Dicyema sepiellae n. sp. is a large species that reaches about 4,000 microm in length. This species lives in folds of the renal appendages. The vermiform stages are characterized as having 24-29 peripheral cells, a conical calotte, and an axial cell that extends to the middle of the metapolar cells, but to the base of propolar cells in vermiform embryos. Infusoriform embryos consist of 39 cells; 2 nuclei are present in each urn cell and the refringent bodies are solid. This is the first description of a dicyemid in any species of Sepiella.

  15. Two new dicyemids from Sepia longipes (Mollusca: Cephalopoda: Decapoda).

    Science.gov (United States)

    Furuya, Hidetaka

    2009-06-01

    Two new species of dicyemid mesozoan are described from Sepia longipes, Sasaki, 1913, collected from Tosa Bay in Japan. Dicyema oxycephalum n. sp. is a medium-sized species that is about 1,800 microm in length. It lives in folds of the renal appendages. The vermiform stages are characterized as having 28-34 peripheral cells, a conical calotte, and an axial cell that extends to the base of the propolar cells. Infusoriform embryos consist of 39 cells; 2 nuclei are present in each urn cell and the refringent bodies are solid. Pseudicyema cappacephalum n. sp. is also a medium-size species; it is about 1,000 microm in length. It too lives in folds of the renal appendages. The vermiform stages are characterized as having 32-34 peripheral cells, a cap-shaped calotte, and an axial cell that extends to the base of propolar cells. Infusoriform embryos consist of 39 cells; 2 nuclei are present in each urn cell and the refringent bodies are solid. This is the first description of dicyemids from S. longipes.

  16. First Zoeal Stage of Camptandrium sexdentatum (Crustacea: Decapoda: Camptandriidae

    Directory of Open Access Journals (Sweden)

    Park, Jay Hee

    2014-10-01

    Full Text Available The first zoea of Camptandrium sexdentatum is described for the first time with a digital image of live zoeas. An ovigerous crab of C. sexdentatum was collected at the muddy sand flat in Namhaedo Island on 2 June 2012 and hatched in the laboratory on 6 June 2012. In Camptandriidae, the first zoea of C. sexdentatum is distinguished from the first zoeas of Cleistostoma dilatatum and Deiratonotus cristatum by having no dorsal and lateral carapace spines, an abdomen significantly broadened posteriorly, and a subovoid telson without forks. Especially, the finding of a subovoid telson without forks is the first report in brachyuran zoeas.

  17. A new cavernicolous freshwater crab from New Guinea (Crustacea Decapoda)

    NARCIS (Netherlands)

    Holthuis, L.B.

    1980-01-01

    Through the kindness of Mr. Philip Chapman of Culford, Bury St. Edmunds, Suffolk, it was made possible for me to examine a male and a female of a species of cavernicolous crab from the Western Highlands of Papua New Guinea, collected there by Mr. N. Plumley. The species proved to be new to science

  18. Review of Palaemoninae (Crustacea: Decapoda: Caridea) from Vietnam, Macrobrachium excepted

    NARCIS (Netherlands)

    Xuan, Van N.

    1992-01-01

    An account is presented of the species of Palaemoninae known from Vietnam, the genus Macrobrachium excluded. Of each species a description or descriptive notes are provided, and the habitat and economic importance are discussed. Illustrations of each species, two of which are new to science, are

  19. The Hermit Crabs (Crustacea Decapoda, Paguridea) of Northwestern North America

    NARCIS (Netherlands)

    Mclaughlin, P.A.

    1974-01-01

    A systematic study has been made of the Paguridea (exclusive of the Lithodidae) from northwestern North America. In addition to the redescriptions of all known species, two subgenera are herein raised to generic rank and a new genus is described. Several systematic problems have been resolved, and

  20. Hermit Crabs (Decapoda: Anomura: Paguridea) from the Seychelles

    NARCIS (Netherlands)

    McLaughlin, P.A.; Hogarth, P.J.

    1998-01-01

    A report on the hermit crabs collected during the Netherlands Seychelles Expedition, 1992-1993, supplemented by materials collected for Enterprise Oil and examined by one of the authors. Twentyseven species, representing three families, are recognized, including one new genus and four new species.

  1. Fecundity of spiny lobster Palinurus gilchristi (Decapoda: Palinuridae)

    African Journals Online (AJOL)

    Fecundity of spiny lobster Palinurus gilchristi was investigated on traditional lobster fishing grounds on the Agulhas Bank, from Mossel Bay to Algoa Bay, and Port Alfred, based on early- and late-stage egg masses collected from 415 mature females (58–118mm carapace length, CL). Fecundity increased linearly with ...

  2. Growth of spiny lobster palinurus gilchristi (decapoda: palinuridae ...

    African Journals Online (AJOL)

    Growth of spiny lobster Palinurus gilchristi was investigated on four traditional lobster fishing grounds, Agulhas Bank, St Francis, Port Elizabeth and Port Alfred, using tagging data. Of 11 144 individuals tagged in six tagging sessions between 1988 and 1993, 1 031 (9.25 %) were recaptured by May 1995. Most P. gilchristi ...

  3. Estructura del aparato reproductor masculino de Uca uruguayensis (Decapoda, Ocypodidae)

    OpenAIRE

    Cuartas,Elena Irene; Petriella,Ana María

    2010-01-01

    The structure of the male reproductive tract was described in Uca uruguayensis Nobili, 1901, by using histological methods, scanning electron microscopy techniques, and stereoscopic observations of fresh material. The aim of this work was to establish the functional changes associated with sexual maturation. The morphology of the first (G1) and second (G2) pair of gonopods was described. The male reproductive tract consists of paired organs: testes, and tubular and convoluted vas deferens (VD...

  4. The Crustacea Decapoda Macrura (the Alpheidae excepted) of Easter Island

    NARCIS (Netherlands)

    Holthuis, L.B.

    1972-01-01

    So far the Crustacean fauna of Easter Island has received but very little attention. In most early narratives of expeditions visiting the island no mention is made of any Crustacean. Behrens (1908: 135) who accompanied Jacob Roggeveen on the voyage during which, in 1722, the island was discovered,

  5. Studies on the morphometry of Macrobrachium nobilii (Decapoda, Palaemonidae

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    Pitchaimuthu Mariappan

    2004-07-01

    Full Text Available Fourteen morphometric characters of Macrobrachium nobilii (Henderson and Matthai, 1910, a freshwater prawn were measured from 72 males and 66 females collected at random in field. The growth constant (b of total length and weight for male (4.28 and female (3.02 differed significantly (PQuatorze características morfológicas do Macrobrachium nobilii (Henderson e Matthai, 1910, 72 machos e 66 fêmeas de lagostas de água fresca foram medidas. O crescimento constante (b do comprimento total e peso para os machos (4.28 e fêmeas (3.02 apresentaram diferenças significativas (P<0.05. A comparação da inclinação e da elevação das regressões entre sexos indicaram diferenças significativas em todos os parâmetros dependentes estudados comprimento da carcaça (x, exceto largura da carcaça (y. Nos machos, as garras principais e secundárias demonstraram alometria positiva quando nas fêmeas o crescimento foi isométrico.

  6. DISTRIBUTION OF AUSTROPOTAMOBIUS TORRENTIUM (DECAPODA: ASTACIDAE IN SLOVAKIA

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    STLOUKAL E.

    2005-07-01

    Full Text Available The distribution of the stone crayfish Austropotamobius torrentium (SCHRANK, 1803 in Slovakia is restricted to only some creeks in the Malé Karpaty Mountains. Most of new sites have been discovered due to intensive investigations during the past three years. The occurrence of this species in other mountain ranges in Slovakia, as mentioned by other authors, has not been confirmed by recent research. A. torrentium is among the rarest species of invertebrates listed in Annex II of the EU Habitats Directive occurring in Slovakia.

  7. Galatheoid squat lobsters (Crustacea: Decapoda: Anomura from Korean waters

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    Jung Nyun Kim

    2016-10-01

    Full Text Available Abstract Ten species of Galatheoidea (squat lobsters, belonging to two families, were collected in the Korean exclusive economic zone: Galathea balssi Miyake and Baba, 1964, Galathea orientalis Stimpson, 1858, Galathea pubescens Stimpson, 1858, and Galathea rubromaculata Miyake and Baba, 1967 belonging to Galatheidae; Bathymunida brevirostris Yokoya, 1933, Cervimunida princeps Benedict, 1902, Munida caesura Macpherson and Baba, 1993, Munida japonica Stimpson, 1858, Munida pherusa Macpherson and Baba, 1993, and Paramunida scabra (Henderson, 1885 belonging to Munididae. The present study comprises the morphological description of these ten species, including drawings and color photographs, a brief review of their regional records, and a key for their identification. Although all species are common in Japanese waters, G. balssi, G. rubromaculata, B. brevirostris, C. princeps, M. caesura, and M. pherusa are new to Korean marine fauna.

  8. Crescimento de Aegla manuinflata (Decapoda, Anomura, Aeglidae em ambiente natural

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    André Trevisan

    2011-12-01

    Full Text Available O presente trabalho teve como objetivo avaliar o crescimento de Aegla manuinflata Bond-Buckup & Santos, 2009 em ambiente natural. Para isso foram realizadas coletas mensais de abril de 2006 a maio de 2007 no arroio Passo Taquara, no município de São Pedro do Sul, Rio Grande do Sul, Brasil. Os organismos foram coletados utilizando armadilhas plásticas e puçá. Os espécimes foram sexados e tiveram mensurado o comprimento do cefalotórax (CC, em seguida foram devolvidos ao córrego. O crescimento foi avaliado utilizando o modelo de von Bertalanffy. As curvas de crescimento do cefalotórax, estimadas para machos e fêmeas, são descritas pelas seguintes equações Ct = 28,00 [1 - e-0,0047(t+10,63] e Ct = 25,16 [1 - e-0,0051(t+17,65], respectivamente. Os machos atingem tamanhos maiores que as fêmeas. A longevidade foi estimada em três anos para os machos e dois anos e meio para as fêmeas.

  9. On some Indo-westpacific Palaemoninae (Crustacea Decapoda Caridae)

    NARCIS (Netherlands)

    Holthuis, L.B.

    1952-01-01

    Since the publication of my paper on the prawns of the subfamily Palaemoninae collected by the Siboga and Snellius Expeditions (Holthuis, 1950, Siboga Exped., mon. 39a9), I had the opportunity to examine more material of this group, which enabled me to make additions and corrections to the above

  10. A technology analysis of the U.S. Atlantic blue crab (Callinectes sapidus) processing industry

    OpenAIRE

    Hong, Gi-Pyo

    1990-01-01

    The dehydration rates of crabs during low temperature storage and various cooking processes were determined. Season, cooking time, and cooking method significantly affected the weight loss of crabs. The thermal energy (F250 ) crabs received during a commercial cooking process was evaluated by season, cooking method and time, and crab type. The effects of sex, size, and type (sexual maturity) of crab and season on the yields of lump, top flake, bottom flake, and claw...

  11. Disease, parasite, and commensal prevalences for blue crab Callinectes sapidus at shedding facilities in Louisiana, USA.

    Science.gov (United States)

    Rogers, Holly A; Taylor, Sabrina S; Hawke, John P; Schott, Eric J; Anderson Lively, Julie A

    2015-01-15

    Blue crab diseases, parasites, and commensals are not well studied in the Gulf of Mexico, and their prevalence rates have only been sporadically determined. Commercial soft shell shedding facilities in Louisiana experience high mortality rates of pre-molt crabs, and some of these deaths may be attributable to diseases or parasites. During the active shedding season in 2013, we determined the prevalence of shell disease, Vibrio spp., Lagenophrys callinectes, and Hematodinium perezi at 4 commercial shedding facilities along the Louisiana coast. We also detected Ameson michaelis and reo-like virus infections. Shell disease was moderately prevalent at rates above 50% and varied by shedding facility, collection month, and crab size. Vibrio spp. bacteria were prevalent in the hemolymph of 37% of the pre-molt crabs. Lagenophrys callinectes was highly prevalent in the pre-molt crabs, but because it is a commensal species, it may not cause high mortality rates. Hematodinium perezi was absent in all pre-molt crabs.

  12. Oesophageal chemoreceptors of blue crabs, Callinectes sapidus, sense chemical deterrents and can block ingestion of food.

    Science.gov (United States)

    Aggio, Juan F; Tieu, Ryan; Wei, Amy; Derby, Charles D

    2012-05-15

    Decapod crustaceans such as blue crabs possess a variety of chemoreceptors that control different stages of the feeding process. All these chemoreceptors are putative targets for feeding deterrents that cause animals to avoid or reject otherwise palatable food. As a first step towards characterizing the chemoreceptors that mediate the effect of deterrents, we used a behavioral approach to investigate their precise location. Data presented here demonstrate that chemoreceptors located on the antennules, pereiopods and mouthparts do not mediate the food-rejection effects of a variety of deterrents, both natural and artificial to crabs. Crabs always searched for deterrent-laced food and took it to their oral region. The deterrent effect was manifested as either rejection or extensive manipulation, but in both cases crabs bit the food. The biting behavior is relevant because the introduction of food into the oral cavity ensured that the deterrents gained access to the oesophageal taste receptors, and so we conclude that they are the ones mediating rejection. Additional support comes from the fact that a variety of deterrent compounds evoked oesophageal dilatation, which is mediated by oesophageal receptors and has been linked to food rejection. Further, there is a positive correlation between a compound's ability to elicit rejection and its ability to evoke oesophageal dilatation. The fact that deterrents do not act at a distance is in accordance with the limited solubility of most known feeding deterrents, and likely influences predator-prey interactions and their outcome: prey organisms will be attacked and bitten before deterrents become relevant.

  13. The effect of salinity on experimental infections of a Hematodinium sp. in blue crabs, Callinectes sapidus.

    Science.gov (United States)

    Coffey, Anna H; Li, Caiwen; Shields, Jeffrey D

    2012-06-01

    The parasitic dinoflagellate Hematodinium sp. parasitizes blue crabs along the Atlantic seaboard of the United States. Infections in blue crabs have only been reported from waters where salinity is >11 practical salinity units (psu). Blue crabs maintain a hyperosmotic internal concentration at low salinities (0-5 psu), roughly comparable to 24 psu, and should be capable of maintaining an infection in low-salinity waters even if Hematodinium spp. cells are intolerant of low salinities. We tested this notion by observing the effect of low salinity on the progression of disease in crabs experimentally infected with the parasite. Blue crabs were acclimated to 5 psu or 30 psu salinity treatments. They were inoculated with Hematodinium sp. and necropsied 3, 7, 10, and 15 days post-inoculation. The low-salinity treatment did not have an effect on the proliferation of Hematodinium sp. infections in blue crabs; moreover, a greater proportion of infections in crabs in the low-salinity treatment developed dinospore stages than did those in the high-salinity treatment, indicating that salinity may affect the development of the parasite. However, dinospores from in vitro cultures rapidly became inactive when held in salinities <15 psu. Our experiments indicate that Hematodinium spp. can develop in blue crabs at low salinities, but that the parasite is incapable of transmission in this environment, which explains the lack of natural infections in crabs at low salinities.

  14. POP levels in blue crab (Callinectes sapidus) and edible fish from the eastern Mediterranean coast.

    Science.gov (United States)

    Tekin, Serkan; Pazi, Idil

    2017-01-01

    Organochlorinated pesticides and Aroclors were measured in the muscle of two edible fish species (gray mullet, sea bream) and blue crab, collected from eastern Mediterranean coast in 2013. The concentration of organochlorinated pesticides (OCPs) and Aroclors in biota samples which were collected at six sites ranged from 1.0-8.6 and 9-47.5 ng g -1 wet weight, respectively. Total DDT concentrations in seafood samples were compared to tolerance level established by the US Food and Drug Administration (FDA); the concentrations were detected below the tolerence level. Health risk assessment was conducted related to the consumption of chemically contaminated seafood. The estimated daily intake of OCPs calculated by using the estimated daily fish consumption in Turkey was far below the acceptable daily intake as established by FAO/WHO. Our data indicated that consumption of blue crab, gray mullet, and sea bream collected from the Mediterranean coast of Turkey could pose "no risk" for human health in terms of OCPs.

  15. Impacts of the Deepwater Horizon oil spill on blue crab, Callinectes sapidus, larval settlement in Mississippi

    Science.gov (United States)

    The largest accidental release of crude oil in history occurred in the north-central Gulf of Mexico (GOM) between April 20 and July 15, 2010 (Alford et al., this volume). The DWH spill was unprecedented due to both its magnitude (>600,000 metric tons released) and its occurren...

  16. Changes in ecdysteroids during embryogenesis of the blue crab, callinectes sapidus rathbun

    Energy Technology Data Exchange (ETDEWEB)

    McCarthy, J.F.; Skinner, D.M.

    1979-01-01

    Total ecdysteroid titers (estimated by radioimmunoassay (RIA)) in embryos of the blue crab increased from approx. 6 ng 20-hydroxyecdysone equivalents/g in the immature embryo to a maximum of approx. 500 ng 20-hydroxyecdysone equivalents/g in maturing embryos; titers dropped to approx. 300 ng 20-hydroxyecydsone equivalents/g in prehatch embryos. High-pressure reverse-phase chromatography of the embryo extracts resolved five RIA-active components, ..cap alpha..-Ecdysone and the polar conjugate of 20-hydroxyecdysone were present in low quantities. The concentration of 20-hydroxyecdysone increased during embryogenesis to a maximum of approx. 160 ng/g in maturing embryos and decreased only slightly in the prehatch embryos. Two unidentified components were also detected and the changes in their concentrations were estimated. One, an apolar component (peak III), accounted for as much as 63% of the total RIA activity as the embryos matured. The estimated concentration of this component increased from 85 ng/g in early embryos to 475 ng/g in maturing embryos, then decreased by 50% in the prehatch embryos. The level of the other, more polar component (peak II) increased from 7.5 to 75 ng/g as the embryos developed. The increase in the concentration of ecdysteroids during embryogenesis indicates that crab embryos have the capacity to synthesize ecdysteroids and suggests that these hormones may have a physiological role in the embryonic development of crustaceans.

  17. Ultrastructure, Histochemistry, and Mineralization Patterns in the Ecdysial Suture of the Blue Crab, Callinectes sapidus

    Science.gov (United States)

    Priester, Carolina; Dillaman, Richard M.; Gay, D. Mark

    2005-12-01

    The ecdysial suture is the region of the arthropod exoskeleton that splits to allow the animal to emerge during ecdysis. We examined the morphology and composition of the intermolt and premolt suture of the blue crab using light microscopy and scanning electron microscopy. The suture could not be identified by routine histological techniques; however 3 of 22 fluorescein isothiocyanate-labeled lectins tested (Lens culinaris agglutinin, Vicia faba agglutinin, and Pisum sativum agglutinin) differentiated the suture, binding more intensely to the suture exocuticle and less intensely to the suture endocuticle. Back-scattered electron (BSE) and secondary electron observations of fracture surfaces of intermolt cuticle showed less mineralized regions in the wedge-shaped suture as did BSE analysis of premolt and intermolt resin-embedded cuticle. The prism regions of the suture exocuticle were not calcified. X-ray microanalysis of both the endocuticle and exocuticle demonstrated that the suture was less calcified than the surrounding cuticle with significantly lower magnesium and phosphorus concentrations, potentially making its mineral more soluble. The presence or absence of a glycoprotein in the organic matrix, the extent and composition of the mineral deposited, and the thickness of the cuticle all likely contribute to the suture being removed by molting fluid, thereby ensuring successful ecdysis.

  18. Record of the freshwater stingrays Potamotrygon brachyura and P. motoro (Chondrichthyes, Potamotrygonidae in the lower Uruguay river, South America Registro das raias de água doce Potamotrygon brachyura e P. motoro (Potamotrygonidae no baixo rio Uruguai, America do Sul

    Directory of Open Access Journals (Sweden)

    María Cristina Oddone

    2012-06-01

    Full Text Available Freshwater stingrays, or potamotrygonids, are restricted to Neotropical river drainages. These elasmobranchs are well adapted to freshwater environments and the number of described species gradually increases as further research is carried out. Some of the first studies on their systematics and natural history were carried out in the 1960s and 1970s in southern South America. However, there is no new published data on potamotrygonids from Uruguayan waters since then (except for local journal reports from sportive fishermen and specimens deposited in Uruguayan collections. The present study aims to record the recent occurrence of two species of potamotrygonids caught by sport fishermen, with comments on other published historical records for the same area. As many other elasmobranchs, these species have an important, but not always well understood, role in the Uruguayan rivers ecosystems.As raias de água doce ou potamotrigonídeos estão restritas às drenagens dos rios Neotropicais. Estas raias tem se adaptado bem aos ambientes de água doce e o número de espécies descritas aumenta gradualmente conforme as pesquisas na área se intensificam. Alguns dos primeiros estudos sobre a sistemática e a historia natural deste grupo foram realizados nas décadas de 1960 e 1970 na região Sul de América do Sul. Porém, há poucos dados publicados sobre potamotrigonídeos em águas uruguaias desde então (com exceção de registros de pescadores esportivos publicados em jornais locais, e espécimes depositados em coleções uruguaias. O presente estudo, portanto, tem como objetivo registrar a ocorrência de duas espécies de potamotrigonídeos no Uruguai capturados por pescadores esportivos assim como realizar uma compilação de ocorrências anteriores presentes na literatura. Como muitos outros elasmobrânquios, estas espécies possuem um papel importante nos ecossistemas dos rios uruguaios, embora não completamente compreendido.

  19. Sexual maturity of the deep-sea red crab Chaceon notialis Manning & Holthuis, 1989 (Brachyura: Geryonidae in southern Brazil Madurez sexual del cangrejo rojo de profundidad Chaceon notialis Manning & Holthuis, 1989 (Brachyura: Geryonidae al sur de Brasil

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    Rodrigo Sant'Ana

    2009-01-01

    Full Text Available The red crab Chaceon notialis is one of the three deep-sea crab species currently exploited in Brazil. The red crab fishery started in 1998 with foreign vessels that, as of 2000, have been extensively moni-tored by observers and tracked by satellite. A management plan implemented in 2005 was based only on bio-mass dynamics, as biological knowledge of the resource was limited at that date. Samples taken aboard were used to determine size at first sexual maturity for males and females by studying the allometric growth of the chelae and abdomen in relation to the carapace width (CW, the proportion of females with opened vulvae and eggs in the pleopods, and males showing copula marks on the first ambulatory legs. Morphometric maturity was attained, on average, at 8.9 cm CW (males and 8.8 cm CW (females. The CW5% was estimated to be 6.9 and 9.7 cm CW for females, considering the vulva condition and eggs in the pleopods, respectively, and 8.4 cm CW for males. The maximum estimated proportions of ovigerous females and males with copula marks by size class were 0.8 and 0.7, respectively, suggesting an annual reproductive cycle for the species, both at the populational and individuals levels. The size composition analysis showed that up to 97% of the females caught in the fishery were immature. Given these results, enhancing trap selectivity and minimizing the mortality of ovigerous females should be considered as new and immediate goals for the management of the resource.El cangrejo-rojo Chaceon notialis corresponde a una de las tres especies de cangrejos de profundidad que actualmente se explotan en Brasil. La pesca de cangrejo-rojo comenzó en el año 1998 por barcos extranjeros que, desde 2000 fueron intensamente vigilados por observadores y rastreados por satélites. En el año de 2005 se implemento un plan de manejo, considerando solamente el estudio de la dinámica de la biomasa del recurso, ya que el conocimiento biológico todavía era limitado. A partir de muestras obtenidas a bordo de los barcos de pesca, se estimó la talla de primera madurez de machos y hembras mediante la utilización del crecimiento alométrico de la quela y el abdomen, con respecto al ancho del caparazón (CW, proporción de hembras con vulvas abiertas y huevos en los pleópodos y machos con marcas de cópula en las primeras patas ambulatorias. La madurez morfométrica para los machos fue obtenida en promedio a 8,9 cm de CW y para las hembras a 8,8 cm de CW. El CW50./d fue estimado en 8,4 cm para machos y para hembras, tomando en cuenta la condición de la vulva o los huevos en los pleópodos, en 6,9 y 9,7 cm respectivamente. Las maximas proporciones estimadas de hembras ovígeras y machos con marcas de cópula por talla fueron de 0,8 y 0,7 respectivamente, lo que sugiere que el ciclo reproductivo a nivel poblacional e individual es anual. El análisis de la composición de tallas indicó que el 97% de las hembras capturadas, eran inmaduras. A partir de estos resultados se consideró un aumento en la selectividad de las trampas y la disminución de las hembras ovígeras en las capturas como objetivos nuevos e inmediatos para el mejor manejo de este recurso.

  20. Sexual maturity of the deep-sea royal crab Chaceon ramosae Manning, Tavares & Albuquerque, 1989 (Brachyura: Geryonidae in southern Brazil Madurez sexual del cangrejo real Chaceon ramosae Manning, Tavares & Albuquerque, 1989 (Brachyura: Geryonidae en el sur de Brasil

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    Paulo Ricardo Pezzuto

    2009-01-01

    Full Text Available The royal crab Chaceon ramosae is one of the three species of deep-sea crabs currently exploited in Brazil. The royal crab fishery started in 2001 with foreign vessels that were extensively monitored by observers and tracked by satellite. A management plan implemented in 2005 was based only on biomass dynamics, as biological knowledge of the resource was limited at that date. Samples taken aboard were used to determine size at first sexual maturity for males and females by studying the use of allometric growth of chelae and abdomen in relation to carapace width (CW, the proportion of females with opened vulvae and eggs in the pleopods, and males showing copula marks on the first ambulatory legs. Morphometric maturity was attained, on average, at 12.1 cm (males and 10.7 cm (females. The CW50% was estimated to be 10.9 cm and 12.2 cm for females, respectively considering the vulva condition and eggs in the pleopods, and 13.6 cm for males. By size class, the maximum estimated proportions of ovigerous females by size class was 0.4 and of males with copula marks was 0.6, suggesting a bi-annual reproductive cycle for individuals of the species. The size composition analysis showed that irnmature individuals may comprise up to 70% of the catches. These results indicate the need to consider enhanced trap selectivity and lower mortality of ovigerous females as new and immediate goals to improve resource management.El cangrejo real Chaceon ramosae corresponde a una de las tres especies de cangrejos de profundidad que actualmente se explotan en Brasil. La pesca de cangrejo real comenzó en el año 2001 por barcos extranjeros que eran intensamente supervisados por observadores y rastreados por satélites. En el año de 2005 se implemento un plan de manejo, considerando solamente el estudio de la dinámica de la biomasa del recurso, ya que el conocimiento biológico todavía era limitado. A partir de muestras obtenidas a bordo de los barcos de pesca, se estimó la talla de primera madurez de machos y hembras a través de la utilización del crecimiento alométrico de la quela y el abdomen con respecto al ancho del caparazón (CW, proporción de hembras con vulvas abiertas y huevos en los pleópodos, y machos con marcas de cópula en las primeras patas ambulatorias. La madurez morfométrica de los machos fue obtenida en promedio a 12,1 cm CW y en las hembras a 10,7 cm CW. El CW50% fue estimado en 13,6 cm en machos y, en hembras considerando la condición de la vulva o los huevos en los pleópodos, en 10,9 y 12,2 cm respectivamente. La máxima proporción estimada de hembras ovígeras y machos con marcas de cópula por talla fueron de 0,4 y 0,6 respectivamente, lo que sugiere que el ciclo reproductivo a nivel individual es bi-anual. El análisis de la composición de tallas evidenció que individuos inmaduros componen hasta el 70% de las capturas. A partir de estos resultados, se consideró incrementar el efecto selectivo de las trampas y disminuir la captura de hembras ovígeras, como objetivos nuevos e inmediatos para mejorar el manejo de este recurso.

  1. Fertilidade do caranguejo de mangue Ucides cordatus (Linnaeus (Crustacea, Brachyura, Ocypodidae, em Iguape (São Paulo, Brasil Fertility of the mangrove crab Ucides cordatus (Linnaeus (Crustacea, Brachyura, Ocypodidae, at Iguape (São Paulo, Brazil

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    Gustavo Yomar Hattori

    2003-06-01

    Full Text Available A total of 58 Ucides cordatus (Linnaeus, 1763 ovigerous females in final embryonic stage were collected in mangrove forest at Iguape (São Paulo. Each female were kept in laboratory until the larvae hatching and had their carapace width (CW measured with caliper (0.05 mm and the larval number (LN calculated by volumetric method. The hatching rates (HR were calculated for each animal's size class (5 mm. The values of LN and CW obtained were subjected to regression analyses and the best fitting model was chosen by comparing the obtained determination coefficients (R². The fertility varied from 71,200 to 220,800 larvae (147,169 ± 32,070 larvae, corresponding to 41.7 to 76.8 mm CW (63.7±7.9 mm, respectively. The relationship LNxCW was expressed by the equation LN = 284.1CW1.5º¹ (N = 58; R² = 0.74; p < 0.01, that showed a positive correlation between the variables. Most part of ovigerous females (89.7% showed their hatching during the nightly period. A negative correlation between HR and CW was observed in these animals (p < 0.05, probably due to reduction of spermatophores in the seminal receptacle (multiparous females or due to senility in the females with large size. The biological studies of U. cordatus are very important for practices adoption that preserve and make the rational use of this recourse in Brazilian's mangrove forests.

  2. Crescimento somático do caranguejo-uçá Ucides cordatus (Crustacea, Brachyura, Ocypodidae em laboratório Somatic growth of the mangrove crab Ucides cordatus (Crustacea, Brachyura, Ocypodidae in laboratory

    Directory of Open Access Journals (Sweden)

    Giovana V. Lima

    Full Text Available Em crustáceos, o aumento de tamanho ocorre imediatamente após a muda, quando o animal está com a carapaça mole. O crescimento de Ucides cordatus (Linnaeus, 1763 foi observado em laboratório, através da ocorrência de muda, incremento de muda e período de intermuda. O estudo foi realizado durante os meses de outubro/2000 a março/2002 e um total de 91 caranguejos (15 machos, 33 fêmeas e 43 juvenis foi coletado no manguezal de Itacuruçá - Coroa Grande, Estado do Rio de Janeiro, Brasil. Os animais foram mantidos em dois tipos de tanques: os caranguejos adultos foram mantidos em tanques de 1000 l, durante 18 meses, enquanto os juvenis foram mantidos em tanques plásticos de 20 l de capacidade durante oito meses. Os animais foram mantidos em sistema aberto de circulação de água do mar e alimentados duas vezes por semana com folhas de Rhizophora mangle (L. (Rhizophoraceae e Laguncularia racemosa (Gaertn. (Combretaceae. A largura da carapaça variou entre 50,1 a 70,0 mm nos machos, 40,2 a 80,0 mm entre as fêmeas e 1,1 a 40,1 mm entre os juvenis. A sobrevivência dos espécimes estudados foi de 46,7% entre os machos, 39,4% entre as fêmeas após dezoito meses e 67,4% entre os juvenis, após oito meses de observação. Os machos e as fêmeas realizaram três mudas durante o experimento, enquanto os juvenis realizaram até duas mudas. As mudas ocorreram entre agosto e abril, mostrando maior freqüência durante a primavera e o verão. O incremento na largura da carapaça diminuiu com o tamanho do indivíduo, com média de 2,21 ± 1,39% para os machos, 1,28 ± 0,84% para as fêmeas e 2,89 ± 2,13% para os juvenis. A relação entre o incremento percentual e a largura da carapaça pode ser expresso pela equação IM = -0.0707LC + 4.645 (r²= 0.40. O período de intermuda foi de 191 ± 140 dias entre os machos, 216 ± 76,2 dias entre as fêmeas e 54 ± 1,41 dia entre os juvenis. O incremento de muda foi estatisticamente significativo (pIn crustaceans, size increase occurs only immediately after molting when the animal is in the soft-shelled condition. The growth of Ucides cordatus (Linnaeus, 1763 was observed in laboratory, through its occurrence of molt, percentual increment of size and intermolt period. The study was realized from October/2000 to March/2002 and a total of 91 crabs (15 males, 33 females and 43 juveniles were sampled in Itacuruçá - Coroa Grande mangrove, State of Rio de Janeiro, Brazil. The animals were maintained in two types of containers: adult crabs were kept in containers of 1000 l capacity, during eighteen months; while the juveniles were kept in plastic containers of 20 l capacity during eight months. The crabs were maintained in flowing seawater and fed on frozen leaves of Rhizophora mangle (L. (Rhizophoraceae and Laguncularia racemosa (Gaertn. (Combretaceae, twice a week. The carapace width ranged from 50.1 to 70.0 mm in males, 40.2 to 80.0 mm to females and 1.1 to 40.1 mm to juveniles. The survival rate of the specimens studied was of 46.7% for males, 39.4% for females after eighteen months and 67.4% for juveniles after eight months. The males and females molted up to three times during the experiment, while the juveniles molted up twice. Molts occurred between August and April, showing more frequency during spring and summer. The increment of carapace width decreased with size, with mean was 2.21 ± 1.39% for males, 1.28 ± 0.84% for females and 2.89 ± 2.13% for juveniles. The relationship between the percentual increment and the carapace width can be expressed by the equation IM = -0.0707LC + 4.645 (r²= 0.40. The intermolt period was 191 ± 140 days for males, 216 ± 76.2 days for females and 54 ± 1.41 days for juveniles. The percentual increment of size was statistically significant (p<0,05 when compared males and females, and adults and juveniles.

  3. Condition factor of Goniopsis cruentata (Crustacea, Brachyura, Grapsidae from Mundaú/Manguaba estuarine complex, Alagoas, Brazil Fator de condição de Goniopsis cruentata (Crustacea, Brachyura, Grapsidae do complexo estuarino Mundaú/Manguaba, Alagoas, Brasil

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    José J. P. R. Lira

    2012-09-01

    Full Text Available The condition factor is a parameter which acts as a general indicator of the "well-being" of a species, and it can be obtained through the analysis of width vs. weight relationships. The present work aims to investigate size vs. weight relationship and the condition factor of the crab Goniopsis cruentata (Latreille, 1803. The study area was the Mundaú/Manguaba estuarine complex, Maceió, state of Alagoas, Northeast Brazil. Samplings were monthly accomplished from August 2007 to July 2008. A total of 626 individuals were analyzed, being 309 males and 317 females. Males were larger and heavier than females, what is expected in many brachyuran. The growth was positive allometric to both males (b = 3.42 and females (b = 3.30, not obeying the "cube law". The condition factor of female was higher than that of male crabs, probably due to the gonad weight of females. It also varied seasonally for both sexes, being higher in the autumn and winter in males, and in the autumn and spring in females, and related to the molt and period of spawning intensification.O fator de condição é um parâmetro que age como um indicador geral do "bem-estar" de uma espécie e pode ser obtido através da análise da relação largura-peso. O presente trabalho visa investigar a relação tamanho vs. peso e o fator de condição do caranguejo Goniopsis cruentata (Latreille, 1803. A área de estudo foi o complexo estuarino Mundaú/Manguaba, Maceió, estado de Alagoas, Nordeste do Brasil. As amostragens foram realizadas mensalmente de agosto de 2007 a julho de 2008. Um total de 626 indivíduos foi analisado, sendo 309 machos e 317 fêmeas. Os machos foram maiores e mais pesados que as fêmeas, o que é esperado em muitas espécies de braquiúros. O crescimento foi alométrico positivo tanto para machos (b = 3,42 quanto para fêmeas (b = 3,30, não obedecendo a "regra do cubo". O fator de condição da fêmea foi maior do que o dos machos, provavelmente devido ao peso das gônadas das fêmeas. O fator de condição também variou sazonalmente para ambos os sexos, sendo mais elevado no outono e no inverno nos machos, e no outono e primavera nas fêmeas, e estando relacionado à muda e ao período intenso de desova.

  4. Maturidade sexual do caranguejo Armases rubripes (Rathbun (Crustacea, Brachyura, Sesarmidae na Baía de Sepetiba, Rio de Janeiro, Brasil Sexual maturity of the crab Armases rubripes (Rathbun (Crustacea, Brachyura, Sesarmidae in Sepetiba Bay, Rio de Janeiro, Brazil

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    Giovana V. Lima

    2006-12-01

    Full Text Available Objetivou-se analisar a maturidade sexual de Armases rubripes (Rathbun, 1897 em três diferentes ambientes (rio, praia arenosa e manguezal no sudeste do Brasil. Os caranguejos foram coletados mensalmente de julho/2002 a fevereiro/2004, utilizando-se peneiras no Rio Sahy durante as marés altas e, manualmente, na praia e no manguezal, durante as marés baixas. Os animais foram capturados por dois coletores num esforço de 15 minutos por estação de coleta. No laboratório, os exemplares foram separados por sexo e mensurados em relação a largura da carapaça, do abdome e comprimento do gonopódio. As gônadas, gonopódios e o abdome foram analisados segundo sua forma e seu aspecto. Um total de 4.051 indivíduos foram amostrados (1.067 machos, 1.563 fêmeas e 1.421 juvenis. Foram determinados cinco morfotipos: juvenis, machos imaturos, fêmeas imaturas, machos maduros e fêmeas maduras. O início da maturidade sexual fisiológica e morfológica ocorreu de forma assíncrona, com machos produzindo gametas mais cedo que as fêmeas. O tamanho da primeira maturidade sexual apresentou diferença significativa entre os ambientes analisados, exceção observada para o manguezal. Os indivíduos obtidos no manguezal apresentaram menores tamanhos para o início da maturidade sexual. Embora, estimados os tamanhos da maturidade sexual morfológica de machos e fêmeas, a análise do crescimento alométrico foi inadequado para a espécie.The present study analyzed the sexual maturity of Armases rubripes (Rathbun, 1897 in three populations from different environmental conditions (river, beach and mangrove in the southeastern Brazilian coast. Crabs were sampled monthly from July 2002 to February 2004; they were captured with sieves at Sahy River, during the high tides, and by hand at beach and mangrove areas, during low tides. Animals were captured by establishing a catch effort of two collectors during 15 minutes for each station. At laboratory, all individuals were sexed and the carapace width measured. The gonads, gonopods and abdomen morphology were observed according to shape and macroscopic aspects. A total of 4,051 specimens were sampled (1,067males, 1,563 females and 1,421 juveniles. There were recognized five morphotypes: juveniles, immature males, immature females, mature males and mature females. The onset of physiological and morphological sexual maturity was asynchronous within sexes, of which, males reaching the ability to produce gametes earlier than females. The size at the onset of sexual maturity was significantly different among populations different coming from areas, except for mangrove area. The individuals obtained from mangrove showed the shortest size at the onset of sexual maturity. Therefore, estimates of males and females sizes at morphological sexual maturity the allometric growth analyses are inadequate in this species.

  5. Aspectos reprodutivos de Goniopsis cruentata (Latreille (Crustacea, Brachyura, Grapsidae na Baía de Sepetiba, Rio de Janeiro, Brasil Reproductive aspects of Goniopsis cruentata (Latreille (Crustacea, Brachyura, Grapsidae at the Sepetiba Bay, Rio de Janeiro, Brazil

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    Zilanda de Souza Silva

    2002-09-01

    Full Text Available The objective of this study was to get some knowledge about the reproductive aspects of Goniopsis cruentata (Latreille, 1803, concerning through sex ratio, morphological sexual maturity, reproductive period, fecundity and eggs size. The crabs were collected monthly in Itacuruçá's Mangroves from November, 1997 to November, 1998. Four hundred and seven crabs were caught and they were 47% males, 36% females and 17% ovigerous females. The sex ratio was 1:1.13 (male:female (X²= 1.54, p > 0.05. The relation of abdomen width (AW and carapace width (CW in females showed a positive allometric grow (b = 1.36, it suggests the size onset of maturity occur from 26.0 a 29.0 mm (CW in females. The ovigerous females occured during all the research period, except for the winter months (June to August. The size of these ovigerous females varied from 26.0 to 48.8 mm with mean size 35.1 + 5.2 mm. Individual fecundity varied from 29,975 to 142,050 eggs and mean fecundity was 74,751 ± 27 eggs. The mean diameter of the eggs was 300 ± 20 µm.

  6. Patterns and Processes of Chasmagnathus granulata and Cyrtograpsus angulatus (Brachyura: Grapsidae) Recruitment in Mar Chiquita Coastal Lagoon, Argentina

    Science.gov (United States)

    Luppi, T. A.; Spivak, E. D.; Anger, K.; Valero, J. L.

    2002-08-01

    Recruitment patterns of crabs and other benthic invertebrates with planktonic larvae are determined by a combination of pre- and post-settlement factors. Recruitment is considered settlement limited when there is a positive correlation between the postlarvae available in the water column and the number of recruits, and regulated when abundance of individuals is mainly affected by post-settlement factors, such as competence and inter or intraspecific predation. Temperate southwestern Atlantic saltmarshes are inhabited by Cyrtograpsus angulatus and Chasmagnathus granulata, two intertidal grapsid crabs. These crabs are considered key species in the ecosystem in Mar Chiquita coastal lagoon, Buenos Aires, Argentina. In this work, we compare the recruitment pattern of Cyrtograpsus angulatus and Chasmagnathus granulata on the basis of a 3-year sampling programme. Furthermore, we examine experimentally, in laboratory and field, settlement and postsettlement processes that can help explain these patterns: habitat selection, intra- and inter-cohort cannibalism and predator avoidance behaviour. Finally, we integrate our results with the previous knowledge about distribution, growth and reproductive biology of both species, to propose a model of recruitment of each species. Both species had a consistent recruitment pattern through 3 years. Recruitment of both species started at the beginning of summer, and continued to autumn, but recruits were present until the beginning of spring. Densities of recruits and juveniles of C. angulatus were not correlated, whereas, there was a relationship between abundance of recruits and juveniles of C. granulata. In the field, recruits of C. angulatus suffered high mortality caused by intra- and inter-cohort cannibalism. Megalopae of C. angulatus selected a substrate (crevices in Ficopomatus enigmaticus reef) that gave them refuge against cannibals. First crabs of C. granulata selected adult conspecific substrate (mud from adult habitats). Recruits of C. angulatus avoided adults of either species. A qualitative model of recruitment of both species is proposed. Post-settlement processes strongly affects the recruitment pattern and, consequently, both species have regulated recruitment. The degree of such regulation, however, is more intense in C. angulatus than in C. granulata.

  7. Consistent habitat segregation between sexes in the spider crabs Maja brachydactyla and Maja squinado (Brachyura, as revealed by stable isotopes

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    Guillermo Guerao

    2016-03-01

    Full Text Available Differences in the resource use patterns of males and females of the spider crab species Maja brachydactyla Balss, 1922 and M. squinado (Herbst, 1788 from several geographic areas (three in the Atlantic and two in the Mediterranean were studied through the analysis of stable isotopes of carbon and nitrogen in the exoskeleton of post-pubertal (adult specimens. Results confirmed that males and females from the same population usually did not differ in δ15N values and hence foraged at the same trophic level. In contrast, females were usually enriched in 13C as compared with males from the same population, thus suggesting that females use shallower habitats than males before the terminal moult. The results reported here also indicate that stable isotop.

  8. Salangathelphusa peractio, a new species of lowland freshwater crab from Pulau Langkawi, Peninsular Malaysia (Crustacea, Brachyura, Gecarcinucidae

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    Peter K. L. Ng

    2017-10-01

    Full Text Available A new species of lowland freshwater crab of the family Gecarcinucidae, Salangathelphusa peractio, is described from Langkawi, an island off the northwestern coast of peninsular Malaysia. Salangathelphusa peractio sp. n. can be separated from S. brevicarinata (Hilgendorf, 1882 in having a proportionately broader external orbital tooth, a distinctly concave posterolateral margin, and the terminal segment of the male first gonopod is not distinctly bent laterally outwards; and from S. anophrys (Kemp, 1923 by its more quadrate carapace and the terminal segment of the male first gonopod possessing a relatively longer and less curved distal part. This is sixth wholly freshwater brachyuran species known from the island.

  9. A new species of Fizesereneia Takeda & Tamura, 1980 (Crustacea: Brachyura: Cryptochiridae) from the Red Sea and Oman

    KAUST Repository

    Van Der Meij, Sancia E T

    2015-03-16

    A new species of cryptochirid crab, Fizesereneia panda van der Meij, is described and illustrated based on specimens collected from the scleractinian corals Lobophyllia cf. hemprichii and L. cf. corymbosa from the Farasan Banks, Farasan Islands, and the reefs off Thuwal in the Saudi Arabian Red Sea, and from Symphyllia recta from reefs in the Gulf of Oman. This is the second cryptochirid species with the Red Sea as type locality. It can be separated from its congeners by the subrectangular carapace, raised midline and the complete division of the carapace depressions, and reddish black colour pattern of these concavities in live specimens. This new species is the seventh assigned to Fizesereneia. A DNA barcode for the new species has been deposited in GenBank. Copyright © 2015 Magnolia Press.

  10. Integrative Taxonomic Study of the Purse Crab Genus Persephona Leach, 1817 (Brachyura: Leucosiidae): Combining Morphology and Molecular Data

    Science.gov (United States)

    Magalhães, Tatiana; Robles, Rafael; Felder, Darryl L.

    2016-01-01

    Marine crabs of the genus Persephona Leach, 1817 are restricted to American waters of the western Atlantic and eastern Pacific Oceans. Subfamilial assignment of this taxon has varied between authors and its species composition remain in question. We conducted a comparative study based on morphology and molecular phylogenetics for all ten recognized species of Persephona, along with Iliacantha hancocki. We tested whether Persephona finneganae, P. lichtensteinii, and P. crinita represent a single species as suggested by some authors; whether specimens identified as P. punctata, P. mediterranea, and P. aquilonaris warrant treatment as separate species; and whether I. hancocki should be regarded as a junior synonym of P. subovata. Diagnostic morphological characters (of the carapace, chelipeds, and third maxillipeds) were used along with gonopod (male first pleopod 1) features and live coloration. The 16S rRNA and the Cytochrome Oxidase I (COI) (DNA barcoding) mitochondrial genes were used as molecular markers. Both morphological and molecular analyses revealed that putative specimens of P. crinita from Brazil and those assigned to P. finneganae were no different from specimens presently assignable to P. lichtensteinii. P. finneganae is regarded as a junior synonym of P. lichtensteinii, and we apply P. crinita only to specimens we examined from the Gulf of Mexico. Specimens from Brazil previously reported as P. crinita are herewith concluded to represent P. lichtensteinii. Additionally, P. townsendi is a junior synonym of P. orbicularis, Iliacantha hancocki is concluded to be a junior synonym of P. subovata, while P. aquilonaris and P. mediterranea are found to represent separate species. On the basis of our revisions, eight species of Persephona are considered valid, and the reported distribution for P. crinita is restricted. PMID:27099956

  11. Morphology of the first zoea of the shamefaced crab Calappa granulata (Linnaeus, 1758 (Brachyura, Calappidae obtained in the laboratory

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    Torres, P.

    1999-12-01

    Full Text Available The first zoeal stage of the calappid crab Calappa granulata is described and illustrated from laboratory-reared material obtained from an ovigerous female captured in the western Mediterranean. The morphology of this larval stage is clearly different from previous description from plankton-collected specimens attributed to this species. The present stage is compared with those previously described from other species of the genus Calappa.En el presente trabajo se describe el primer estadio larvario del cangrejo Calappa granulata. Las larvas se obtuvieron a partir de una hembra ovígera capturada en el Mediterráneo occidental. La morfología de la primera zoea es claramente diferente a la anterior descripción realizada a partir de ejemplares obtenidos en el plancton, y que fue atribuida a C. granulata. La primera zoea de esta especie es comparada con la de otras especies descritas del género Calappa.

  12. Population structure and sexual maturity of the calico box crab Hepatus epheliticus Linnaeus (Brachyura, Hepatidae) from Yucatan Peninsula, Mexico

    OpenAIRE

    Hernaez, Patricio; Rombenso,Artur; Pinheiro, Marcelo A. A.; Simoes, Nuno

    2012-01-01

    The calico box crab Hepatus epheliticus is an abundant species from shallow and continental shelf waters of the Atlantic coast of USA and Mexico. Information about population structure and sexual maturity is absent, even though this crab is caught to be used as bait for the octopus fishery in the Campeche Bank, Mexico. In order to achieve such information, a total of 768 individuals were collected from January to March 2010 through baited traps installed in the Yucatan Peninsula, Mexico. Our ...

  13. Morphology and histology of the female reproductive system of the mangrove land crab, Ucides cordatus (Linnaeus, 1763 (Brachyura: Ocypodidae

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    Flávia Duarte Ferraz Sampaio

    Full Text Available This study provides a detailed description of the macro- and microscopic anatomy of the female reproductive system of the mangrove land crab, Ucides cordatus. A total of 277 females were collected between October 1998 and September 1999 in mangroves of the Victoria Bay, State of Espírito Santo, Southeast Brazil. The reproductive system of this species is composed of two ovaries united together by a bridge, present in the form of an H when viewed dorsally, and two seminal receptacles. Histological analysis revealed the following six stages of oocyte development: I. young germ cells (oogonias; II. oocytes of the germinative centers; III. oocytes with lipid vitellogenesis; IV. vitellogenic oocytes with lipid and protein; V. oocytes with completed vitellogenesis; and VI. oocytes in hyalinization. The anatomy of the ovary is characterized by the following six stages of development: I. immature; II. at maturity; III. mature; IV. partly spawned; V. fully spawned; and VI. in recovery. Reproduction was observed in summer, and the results suggesting that this species showed multiple spawnings.

  14. A molecular method for the detection of sally lightfoot crab larvae (Grapsus grapsus, Brachyura, Grapsidae) in plankton samples.

    Science.gov (United States)

    Ströher, Patrícia R; Firkowski, Carina R; Freire, Andrea S; Pie, Marcio R

    2011-07-01

    The decapod Grapsus grapsus is commonly found on oceanic islands of the Pacific and Atlantic coasts of the Americas. In this study, a simple, quick and reliable method for detecting its larvae in plankton samples is described, which makes it ideal for large-scale studies of larval dispersal patterns in the species.

  15. Biology of Grapsus grapsus (Linnaeus, 1758) (Brachyura, Grapsidae) in the Saint Peter and Saint Paul Archipelago, Equatorial Atlantic Ocean

    National Research Council Canada - National Science Library

    Freire, A S; Pinheiro, M A. A; Karam-Silva, H; Teschima, M M

    2011-01-01

    .... The low diversity of food items, absence of predators of large crabs and high geographic isolation are the determinants of unique behavioural and biological characteristics observed in the G. grapsus population.

  16. A molecular method for the detection of sally lightfoot crab larvae (Grapsus grapsus, Brachyura, Grapsidae) in plankton samples

    OpenAIRE

    Ströher,Patrícia R.; Carina R. Firkowski; Freire,Andrea S.; Pie, Marcio R.

    2011-01-01

    The decapod Grapsus grapsus is commonly found on oceanic islands of the Pacific and Atlantic coasts of the Americas. In this study, a simple, quick and reliable method for detecting its larvae in plankton samples is described, which makes it ideal for large-scale studies of larval dispersal patterns in the species.

  17. Moult cycle and growth of the crab Halicarcinus planatus (Brachyura, Hymenosomatidae) in the Beagle Channel, southern tip of South America

    Science.gov (United States)

    Diez, Mariano J.; Lovrich, Gustavo A.

    2013-09-01

    The crab Halicarcinus planatus is the only hymenosomatid crab that inhabits the southern tip of South America and is the only decapod species that reproduces twice a year in the Beagle Channel. In this article, we study the moult cycle in the field (moult frequency, analysis of size frequency distribution) and linked it with growth studied in the laboratory (absolute and per cent growth increment, Hiatt function). Hiatt functions were similar for males and females. Moult frequency was seasonal: in early austral spring and in austral summer. In females, the pubertal moult is the terminal moult, whereas males continue moulting after attaining the size of morphometric maturity. Moult increment was highly variable. The relationship between absolute moult increment and crab size was described by a quadratic function. Per cent growth increment decreased with size, and relationships were different for each sex: linear for females and quadratic for males. Seven and eight modal groups explained the size frequency distributions for females and males from the field, respectively, and revealed the existence of two cohorts of recruits per year. Further modal analysis was mainly hampered by the high variability of size increment that could make any moulting individual fall in its own or one of two following modal groups. The antagonism between growth and reproduction was evident in small males. We hypothesize that the terminal pubertal moult is an advantageous feature that allows females to maximize their investment in reproduction after their terminal moult, which allows this species to have two spawnings per year.

  18. Cangrejos braquiuros (Brachyura del arrecife de Cabo Pulmo-Los Frailes y alrededores, Golfo de California, México

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    María del Socorro García-Madrigal

    1999-06-01

    Full Text Available El análisis de los fragmentos del coral Pocillopora elegans recolectados en siete campañas de muestreo durante 1988 y 1989 resultó en 281 ejemplares de braquiuros, identificándose 30 especies de ocho familias, de las cuales 22 especies son nuevos registros para el área. Había 39 especies de braquiuros registradas; ahora hay 61 especies de braquiuros, repartidas en 16 familias. Majidae contiene el mayor número de especies (23, seguida por Panopeidae (9 y Xanthidae (6. Las especies más abundantes fueron Trapezia ferruginea (71 ejemplares, Theleophrys cristulipes (39 y Microcassiope xantusii xantusii (29. Todas las especies se agruparon en cuatro grupos zoogeográficos: indopacífico (8.5%, anfiamericano (5.1%, Pacífico oriental (76.3% y endémico del Pacífico mexicano (10.1%. Las especies de braquiuros de este arrecife (con sólo 0.004% de la superficie de la región representan el 21% de todas las especies registradas para la región del golfo de California, costa occidental de Baja California Sur, áreas costeras de Nayarit y Sinaloa y zona económica exclusiva de estos estados.Fragments of the coral Pocillopora elegans collected in 1988 and 1989 produced 281 specimens of brachyurans. There were 30 species and subspecies in eight families, and 22 are new records for the Cabo Pulmo-Los Frailes reef. Including earlier records, there are now 61 species and subspecies (19 families from the area. Majidae had the largest number of species (23, followed by Panopeidae (9, and Xanthidae (6. Most abundant were Trapezia ferruginea (71 specimens, Theleophrys cristulipes (39 and Microcassiope xantusii xantusii (29. The species were sorted out in four zoogeographic groups: Indopacific (8.5%, Amphiamerican (5.1%, Eastern Pacific (76.3%, and Mexican Pacific endemics (10.1%. The reef represents only 0.004% of the Gulf area but harbors 21% of all species recorded from the gulf of California, western coast of Baja California Sur and exclusive economic zone of Nayarit and Sinaloa.

  19. Cangrejos braquiuros (Brachyura de la bahía de Maruata, Michoacán, México

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    María del Socorro García-Madrigal

    2000-03-01

    Full Text Available Se hicieron 11 muestreos mensuales, de abril de 1990 a marzo de 1991. Las recolectas se hicieron en forma manual en todos los ambientes: fango, arena, vegetación, laguna, río y en la zona intermareal, hasta una profundidad máxima de seis metros. Se obtuvieron 287 ejemplares de braquiuros agrupados en 14 familias, 34 géneros y 42 especies. Las especies analizadas son nuevos registros geográficos para las costas de Michoacán, además de dos ampliaciones de ámbito: una, Micropanope cristimanus hacia el sur desde Manzanillo, Colima; la otra, Glyptograpsus impressus, hacia el norte desde Acapulco, Guerrero. Se proporciona una nueva localidad intermedia para tres especies con distribución disyunta, Eupleurodon peruvianus, Epialtus sulcirostris y Mithrax spinipes. Las familias con mayor número de especies son la Mithracidae (7 y Grapsidae (6. Las especies fueron agrupadas en cuatro grupos biogeográficos: Indopacífico (4.76%, Anfiamericano (9.52%, Pacífico este tropical (78.57% y Pacífico mexicano (7.14%.A total of 287 specimen of brachyuran crabs were collected in 1990-1991. The specimens belong to 42 species, 34 genera and 14 families. All species represent new records for the coast of Michoacan and include two range extensions: Micropanope cristimanus from Manzanillo to Maruata, and Glyptograpsus impressus from Acapulco to Maruata. The presence of Eupleurodon peruvianus, Epialtus sulcirostris and Mithrax spinipes represents a gap in their distribution. The families with haighest numbers of species were the Mithracidae (7 and Grapsidae (6. The species were sorted out in four groups acording to their zoogeographic affinity: Indopacific (4.76%, Amphiamerican (9.52%, Eastern Pacific (78.57% and Mexican Pacific (7.14%.

  20. Harryplax severus, a new genus and species of an unusual coral rubble-inhabiting crab from Guam (Crustacea, Brachyura, Christmaplacidae

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    Jose C.E. Mendoza

    2017-01-01

    Full Text Available Harryplax severus, a new genus and species of coral rubble-dwelling pseudozioid crab is described from the island of Guam in the western Pacific Ocean. The unusual morphological features of its carapace, thoracic sternum, eyes, antennules, pereopods and gonopods place it in the family Christmaplacidae Naruse & Ng, 2014. A suite of characters on the cephalothorax, pleon and appendages distinguishes H. severus gen. & sp. n. from the previously sole representative of the family, Christmaplax mirabilis Naruse & Ng, 2014, described from Christmas Island in the eastern Indian Ocean. This represents the first record of Christmaplacidae in the Pacific Ocean. With the discovery of a second genus, a revised diagnosis for Christmaplacidae is provided.

  1. Distribution notes on the Endangered freshwater crab Ceylonthelphusa armata Ng (1995 (Crustacea: Brachyura: Gecarcinucidae in Sri Lanka

    Directory of Open Access Journals (Sweden)

    U. S.C. Udagedera

    2015-09-01

    Full Text Available There are 52 species of freshwater crabs in Sri Lanka and 51 of them are endemic. There are several species that are known only from a single locality.  Ceylonthelphusa armata is such a species, and we have initiated a study to record the distribution of this species as its typical locality is heavily polluted at Kadugannawa.  At present C. armata is known from six localities, and this information will be useful to facilitate the conservation of the species by assessing the Red List.  

  2. A molecular method for the detection of sally lightfoot crab larvae (Grapsus grapsus, Brachyura, Grapsidae in plankton samples

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    Patrícia R. Ströher

    2011-01-01

    Full Text Available The decapod Grapsus grapsus is commonly found on oceanic islands of the Pacific and Atlantic coasts of the Americas. In this study, a simple, quick and reliable method for detecting its larvae in plankton samples is described, which makes it ideal for large-scale studies of larval dispersal patterns in the species.

  3. Range extensions along western Atlantic for Epialtidae crabs (Brachyura, Majoidea genera Acanthonyx Latreille, 1828 and Epialtus H. Milne Edwards, 1834

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    Ana Francisca Tamburus

    Full Text Available The present study provided information extending the known geographical distribution of three species of majoid crabs, the epialtids Acanthonyx dissimulatus Coelho, 1993, Epialtus bituberculatus H. Milne Edwards, 1834, and E. brasiliensis Dana, 1852. Specimens of both genera from different carcinological collections were studied by comparing morphological characters. We provide new data that extends the geographical distributions of E. bituberculatus to the coast of the states of Paraná and Santa Catarina (Brazil, and offer new records from Belize and Costa Rica. Epialtus brasiliensis is recorded for the first time in the state of Rio Grande do Sul (Brazil, and A. dissimulatus is reported from Quintana Roo, Mexico. The distribution of A. dissimulatus, previously known as endemic to Brazil, has a gap between the states of Espírito Santo and Rio de Janeiro. However, this restricted southern distribution is herein amplified by the Mexican specimens.

  4. Reproduction and management of the mangrove crab Ucides cordatus (Crustacea, Brachyura, Ucididae at Iguape, São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    BRUNO S. SANT'ANNA

    2014-09-01

    Full Text Available The mangrove crab Ucides cordatus is one of the most exploited crustaceans in Brazil. The present study investigated the breeding season of this species and the period of the “andada” phenomenon, when the crabs are active outside their burrows and perform agonistic behaviors. Furthermore related them to environmental factors, in a mangrove on the coast of Brazil, with inferences about management of this crab. The crabs reproduced from October through February. The “andada” occurred from November through February, with typical characteristics in the first two months, during the full and new moon periods, and was influenced positively by the air and soil temperature, luminosity, and tidal amplitude, and negatively by salinity. Based on the results of the present study, the period when the “andada” occurs in other parts of Brazil could be defined, which will aid in managing this fishery. For Iguape, state of São Paulo, Brazil, we suggest a legal off-season for both sexes in December or during the full and new moon, including January.

  5. Condition factor of Goniopsis cruentata (Crustacea, Brachyura, Grapsidae from Mundaú/Manguaba estuarine complex, Alagoas, Brazil

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    José J. P. R. Lira

    2012-09-01

    Full Text Available The condition factor is a parameter which acts as a general indicator of the "well-being" of a species, and it can be obtained through the analysis of width vs. weight relationships. The present work aims to investigate size vs. weight relationship and the condition factor of the crab Goniopsis cruentata (Latreille, 1803. The study area was the Mundaú/Manguaba estuarine complex, Maceió, state of Alagoas, Northeast Brazil. Samplings were monthly accomplished from August 2007 to July 2008. A total of 626 individuals were analyzed, being 309 males and 317 females. Males were larger and heavier than females, what is expected in many brachyuran. The growth was positive allometric to both males (b = 3.42 and females (b = 3.30, not obeying the "cube law". The condition factor of female was higher than that of male crabs, probably due to the gonad weight of females. It also varied seasonally for both sexes, being higher in the autumn and winter in males, and in the autumn and spring in females, and related to the molt and period of spawning intensification.

  6. DIVERSIDADE E DISTRIBUIÇÃO DOS PORTUNIDEOS (CRUSTACEA, BRACHYURA NO LITORAL DE ANCHIETA, ESPÍRITO SANTO, BRASIL

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    Renzo Gonçalves Tavares

    2017-01-01

    Full Text Available O objetivo deste estudo foi caracterizar a diversidade e a distribuição espaço temporal dos portunideos na região de Anchieta, litoral sul capixaba, relacionando com fatores ambientais como: temperatura, salinidade, profundidade, teor de matéria orgânica e textura do sedimento. Os indivíduos foram coletados no período de Março/2013 à Fevereiro/2015 em três pontos distintos. Não houve diferença significativa nos valores médios de temperatura, salinidade e teor de matéria orgânica. Foi coletado um total de 2.849 portunideos, compreendendo quatro espécies: Callinectes ornatus e Callinectes danae que foram consideradas constantes e Cronius ruber e Achelous spinimanus consideradas acessórias. A maior abundância de indivíduos foi registrada no P1 por ser próximo ao estuário, região importante para o desenvolvimento e ciclo de vida desses animais, e com picos no outono para C. ornatus e na primavera para C. danae. A região de Anchieta pode ser considerada uma área importante para o estabelecimento e manutenção dessas espécies de portunideos.

  7. Cytochemical characterization of gill and hepatopancreatic cells of the crab Ucides cordatus (Crustacea, Brachyura validated by cell metal transport

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    Priscila Ortega

    2014-09-01

    Full Text Available Ucides cordatus (Linnaeus, 1763 is a hypo-hyper-regulating mangrove crab possessing gills for respiratory and osmoregulatory processes, separated in anterior and posterior sections. They also have hepatopancreas, which is responsible for digestion and absorption of nutrients and detoxification of toxic metals. Each of these organs has specific cells that are important for in vitro studies in cell biology, ion and toxic metals transport. In order to study and characterize cells from gills and hepatopancreas, both were separated using a Sucrose Gradient (SG from 10 to 40% and cells in each gradient were characterized using the vital mitochondrial dye DASPEI (2-(4-dimethylaminostyryl-N- ethylpyridinium iodide and Trichrome Mallory's stain. Both in 20 and 40% SG for gill cells and 30% SG for hepatopancreatic cells, a greater number of cells were colored with DASPEI, indicating a larger number of mitochondria in these cells. It is concluded that the gill cells present in 20% and 40% SG are Thin cells, responsible for respiratory processes and Ionocytes responsible for ion transport, respectively. For hepatopancreatic cells, the 30% SG is composed of Fibrillar cells that possess larger number of membrane ion and nutrient transporters. Moreover, the transport of toxic metal cadmium (Cd by isolated hepatopancreatic cells was performed as a way of following cell physiological integrity after cell separation and to study differences in transport among the cells. All hepatopancreatic cells were able to transport Cd. These findings are the first step for further work on isolated cells of these important exchange epithelia of crabs, using a simple separation method and to further develop successful in vitro cell culture in crabs.

  8. A new species of Trichopeltarion A. Milne-Edwards, 1880, from the Southwestern Atlantic (Crustacea: Brachyura: Atelecyclidae

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    Marcos Tavares

    2005-01-01

    Full Text Available A new species of Trichopeltarion A. Milne-Edwards, 1880, is described from off coast of Brazil, namely Trichopeltarion pezzutoi n. sp. The new species is compared to its Atlantic congeners, Trichopeltarion nobile A. Milne-Edwards, 1880, and Trichopeltarion intesi (Crosnier, 1981. The record of T. nobile from Brazil should actually be attributed to T. pezzutoi n. sp. The differences between the genera Trichopeltarion and Peltarion Jacquinot, 1847 are discussed.Uma nova espécie do gênero Trichopeltarion A. Milne-Edwards, 1880 coligida no talude continental brasileiro é descrita e ilustrada, nomeadamente Trichopeltarion pezzutoi n. sp. A nova espécie é comparada às suas congêneres do oceano Atlântico, Trichopeltarion nobile A. Milne-Edwards, 1880 e Trichopeltarion intesi (Crosnier, 1981. O registro de T. nobile para o Brasil deve ser atribuído à T. pezzutoi n. sp. São discutidas as diferenças entre os gêneros Trichopeltarion e Peltarion Jacquinot, 1847.

  9. A new species of Fizesereneia Takeda & Tamura, 1980 (Crustacea: Brachyura: Cryptochiridae) from the Red Sea and Oman

    NARCIS (Netherlands)

    Meij, van der S.E.T.; Berumen, M.L.; Paulay, G.

    2015-01-01

    A new species of cryptochirid crab, Fizesereneia panda van der Meij, is described and illustrated based on specimens collected from the scleractinian corals Lobophyllia cf. hemprichii and L. cf. corymbosa from the Farasan Banks, Farasan Islands, and the reefs off Thuwal in the Saudi Arabian Red Sea,

  10. Combined Effects of Temperature and Salinity on Larval ...

    African Journals Online (AJOL)

    Daisy Ouya

    Decapoda,. Grapsidae). Scient. Mar. 54: 55–60. Anger, K., Harms, J., Montú, M. & De Bakker, C. (1990) Effects of salinity on the larval development of a semiterrestrial tropical crab,. Sesarma angustipes (Decapoda: Grapsidae). Mar. Ecol. Prog.

  11. Estructura del aparato reproductor masculino de Uca uruguayensis (Decapoda, Ocypodidae) Structure of the male reproductive tract of Uca uruguayensis (Decapoda, Ocypodidae)

    National Research Council Canada - National Science Library

    Elena Irene Cuartas; Ana María Petriella

    El objetivo de este trabajo es describir las estructuras del sistema reproductor de machos de Uca uruguayensis Nobili, 1901 y estudiar los cambios relacionados con la madurez sexual. Se trabajó con observación...

  12. Estrutura populacional e biologia reprodutiva de Parastacus defossus (Crustacea: Decapoda: Parastacidae Population structure and reproductive biology of Parastacus defossus (Crustacea: Decapoda: Parastacidae

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    Clarissa K. Noro

    2008-12-01

    Full Text Available Este trabalho descreve a estrutura populacional e aspectos da biologia reprodutiva de Parastacus defossus Faxon, 1898, uma espécie com hábito fossorial que passa sua vida no interior de galerias subterrâneas. Aproximadamente 30 exemplares foram coletados mensalmente, de janeiro de 2003 a agosto de 2005 na região do Lami, Município de Porto Alegre, Rio Grande do Sul, Brasil. Registrou-se o comprimento mínimo, máximo e médio da carapaça de machos, fêmeas, juvenis e animais com ootestículo. Analisou-se a distribuição de freqüência por classes de tamanho, a proporção sexual, o recrutamento e o período reprodutivo. Foram amostrados um total de 766 exemplares, 315 machos (41,12%, 278 fêmeas (36,30%, 166 juvenis (21,67% e sete animais com ootestículo (0,91%. Machos e fêmeas estiveram presentes durante todos os meses de amostragem e em todas as classes de tamanho, sendo que a maior freqüência de indivíduos foi registrada nas classes de tamanho ente 24 a 30 mm. A maior freqüência de juvenis foi observada na primavera/2003 e verão/2005. Diferenças significativas no tamanho de machos e fêmeas foram registradas apenas nas classes de tamanho entre 26-30 mm. A proporção sexual no período amostral foi de 1:0,88 (x² = 2,3; p > 0,05 e entre as classes de tamanho foram constatadas diferenças significativas nas classes de tamanho 26-28 mm (x² = 5,43; p This study investigates the population structure and the reproductive biology of Parastacus defossus Faxon, 1898, a fossorial species that spends its life inside subterranean tunnels. Around 30 specimens were collected each month in the region of Lami, municipality of Porto Alegre, state of Rio Grande do Sul, Brazil, from January, 2003 to August, 2005. The minimum, the maximum, and the mean carapace length of males, females, juveniles and individuals with ootestis were recorded. The frequency distribution by size classes, the sexual proportion, the recruitment, and the reproductive period were analyzed. A total of 766 specimens were collected, from which 315 were males (41.12%, 278 were females (36.30%, 166 were juveniles (21.67%, and seven individuals had ootestis (0.91%. Males and females were present in all months and in all size classes. The highest frequency of individuals matched the size classes from 24 to 30 mm. The highest frequency of juveniles was recorded during the spring of 2003 and the summer 2005. Significant differences in the size of males and females were recorded in the size classes between 26 and 30 mm. The sex ratio between all males and females was 1:0.88 (x² = 2.3, p > 0.05. The proportion between males and females showed significant differences between the size classes 26-28 mm (x² = 5.43, p < 0.05 and 28-30 mm (x² = 4.77, p < 0.05 carapace length, were the number of males was highest. Of the 278 females, only 17 were ovigerous (6.11% and the reproductive period was clearly restricted to the spring.

  13. The effects of power station entrainment passage on three species of marine planktonic crustacean, Acartia tonsa (Copepoda), Crangon crangon (Decapoda) and Homarus gammarus (Decapoda).

    Science.gov (United States)

    Bamber, Roger N; Seaby, Richard M H

    2004-05-01

    Experiments have been undertaken exposing larval common shrimp (Crangon crangon) and lobster (Homarus gammarus) and adult copepods (Acartia tonsa) to the key stresses of entrainment within power-station cooling-water systems. The apparatus has enabled the testing of mechanical, thermal, chlorine and realistic pressure effects both alone and in combination, the range of stressors spanning the standard conditions found within a temperate coastal direct-cooled power station. Mechanical stresses affected only lobster larvae, pressure changes affected only the Acartia adults. Residual chlorine caused significant mortality of Acartia and shrimp larvae, but had no effect on lobster larvae even at 1 ppm. The temperature increment significantly affected all three species, with a synergistic effect on chlorine sensitivity in the shrimp larvae, but only temperatures higher than would be experienced in a normally-operating power station affected the copepods. The majority of individuals of each species would survive passage through a power-station system under normal conditions. It is notable that, within the species tested, generalizations from the responses of one species to those of another are not valid.

  14. Palaemonidae (Crustacea: Decapoda: Caridea from the shallow waters from Quintana Roo, Mexican Caribbean coast Palaemonidae (Crustacea: Decapoda: Caridea de las aguas someras de Quintana Roo, Caribe mexicano

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    Ramiro Román-Contreras

    2010-04-01

    Full Text Available Few studies have focused on the palaemonid fauna of the Mexican Caribbean. This study provides a list of shallow water free-living and symbiont shrimps of the family Palaemonidae collected on turtle grass (Thalassia testudinum in Bahía de la Ascensión, Bahía del Espíritu Santo and Mahahual reef lagoon, Quintana Roo, Mexico. Ten species in 8 genera are reported, of which the genus Periclimenes is the most diverse with 3 species. An updated geographic distribution along the western Atlantic and other regions is provided for all the species. The greatest affinity of the palaemonid fauna studied, besides that with the Caribbean province, is with the Brazilian, Argentinian, and Texan zoogeographic provinces. Of the 10 species reported in this paper, 8 represent new local records in the studied area.Los crustáceos de la familia Palaemonidae del Caribe mexicano han sido poco estudiados. En este trabajo se presenta un listado de palemónidos de vida libre y simbiontes recolectados en el pasto marino Thalassia testudiuim de Bahía de la Ascensión, Bahía del Espíritu Santo y la laguna arrecifal de Mahahual, Quintana Roo, México. Se registran 10 especies pertenecientes a 8 géneros, siendo el género Periclimenes el más diverso con 3 especies. Para todas las especies se proporciona su distribución geográfica en la costa del Atlántico americano así como en otras regiones. Además de la provincia Caribeña, los palemónidos recolectados también muestran alta afinidad con otras provincias zoogeográficas como la Brasileña, Argentina y Texana; de las 10 especies registradas en este estudio, 8 representan nuevo registro local en el área estudiada.

  15. Dynamics of gonadal development of Aegla platensis Schmitt (Decapoda, Anomura, Aeglidae Dinâmica do desenvolvimento gonadal de Aegla platensis Schmitt (Decapoda, Anomura, Aeglidae

    Directory of Open Access Journals (Sweden)

    Carolina C. Sokolowicz

    2006-12-01

    Full Text Available One way to estimate gonadal development through the reproductive cycle is to observe the growth of the gonads related to the organs used to store energy. The aim of this study was to follow the gonadosomatic and hepatosomatic indexes during annual cycle of Aegla platensis Schmitt, 1942. Adult animals were collected in Taquara, Rio Grande do Sul, Brazil (29°46'S, 50°53'W. Males and females were initially weighed and dissected and had their gonads and hepatopancreas (HP removed and weighed in order to estimate the Gonadosomatic (GI and hepatosomatic (HI indexes. In females, the indexes were also compared to the degree of development of the ovaries. In males the GI showed a peak of gonadal development in the autumn (p Uma maneira de estimar o desenvolvimento gonadal ao longo do ciclo reprodutivo é observar o crescimento das gônadas em relação aos órgãos utilizados para o estoque de energia. O objetivo desse estudo foi acompanhar os índices gonadossomático e hepatossomático durante o ciclo reprodutivo de Aegla platensis Schmitt, 1942. Animais adultos foram coletados em Taquara, Rio Grande do Sul, Brasil (29°46'S, 50°53'W. Machos e fêmeas foram pesados e dissecados, gônadas e hepatopâncreas (HP foram retirados e pesados para obtenção dos índices gonadossomático (IG e hepatossomático (IH. Nas fêmeas, os índices também foram comparados com o grau de desenvolvimento dos ovários. Nos machos o IG mostrou um pico de desenvolvimento gonadal no outono (p < 0,05, quando os valores do IH diminuíram (p < 0,05. Fêmeas mostraram um aumento do IG no final do verão e início do outono (p < 0,05 e os valores do IG aumentaram à medida que o ovário tornava-se maduro. Em A. platensis, tanto nos machos como nas fêmeas, os valores do IH nunca mostraram valores mais baixos que o IG, o que pode indicar que esses aeglídeos apresentam um padrão de utilização de energia diferente dos outros decápodos, onde à medida que o desenvolvimento gonadal aumenta os valores do IH diminuem marcadamente.

  16. Notes on a collection of Crustacea Decapoda from the Great Bitter Lake, Egypt, with a list of the species of Decapoda known from the Suez Canal

    NARCIS (Netherlands)

    Holthuis, L.B.

    1956-01-01

    Between August 18 and September 5, 1950, Dr. C. Beets, geologist Royal Dutch Shell Oil Company, explored the aquatic fauna and flora of the Great Bitter Lake. In the course of this exploration dredge hauls were made at 47 stations, distributed all over the lake. An account of this work and a

  17. Reproductive behavior of intertidal hermit crabs (Decapoda, Anomura in southeastern Brazil Comportamento reprodutivo de ermitões (Decapoda, Anomura entremarés no sudeste brasileiro

    Directory of Open Access Journals (Sweden)

    Alexander Turra

    2005-06-01

    Full Text Available The reproductive behavior of four intertidal hermit crab species [Clibanarius antillensis Stimpson, 1859, C. sclopetarius (Herbst, 1796, C. vittatus (Bosc, 1802, and Pagurus criniticornis (Dana, 1852] was studied in São Sebastião Channel, southeastern Brazil. The overall behavior followed previous descriptions for Diogenid and Pagurid hermit crabs but some particular differences were recorded. Male to male agonistic encounters over females and guarding behavior were more common to C. sclopetarius, C. vittatus, and Pagurus criniticornis. During guarding, males clearly grasped females' chelipeds, eyestalks, and the margins of their shell apertures. This oblique guarding and, simultaneously, accessing behavior (OGAP has never been described for hermit crabs and was more common to C. sclopetarius and C. vittatus. Pagurus criniticornis did not present the typical jerking behavior of pagurids. Multiple copulations between a single pair were recorded in all species while in only one occasion a female of C. vittatus was observed copulating with two males. In the post-copulatory behavior males held females for some time and, after that, they were left inactive and sometimes totally to partially buried in the substrate. In mating pairs of all species males were larger than females and two individuals (out of 23 of C. antillensis, one (out of 5 of C. sclopetarius and one (out of 3 of C. vittatus, which courted successfully as males fertilizing females' eggs, were demonstrated to be intersex, with both male and female gonopores.O comportamento reprodutivo de quatro espécies de caranguejos ermitões entremarés [Clibanarius antillensis Stimpson, 1859, C. sclopetarius (Herbst, 1796, C. vittatus (Bosc, 1802 e Pagurus criniticornis (Dana, 1852] foi estudado no Canal de São Sebastião, sudeste brasileiro. O comportamento geral destas espécies seguiu descrições prévias para ermitões das famílias Diogenidae e Paguridae mas algumas diferenças foram registradas. Encontros agonísticos entre machos para disputar fêmeas e o comportamento de guarda foram mais comuns a C. sclopetarius, C. vittatus e Pagurus criniticornis. Durante a guarda os machos claramente tocavam os quelípodos, pedúnculos oculares e as margens da abertura das conchas das fêmeas. Este comportamento de guardar e, simultaneamente, estimular as fêmeas em uma posição oblíqua (OGAP nunca havia sido registrado e, no presente estudo, foi mais conspícuo em C. sclopetarius e C. vittatus. Pagurus criniticornis não apresentou o comportamento típico da família Paguridae denominado "jerking" (machos sacudindo as fêmeas. Cópulas múltiplas em um mesmo par foram registradas para todas as espécies enquanto apenas em uma ocasião uma fêmea de C. vittatus foi copulada por dois machos diferentes. Após a cópula os machos continuavam segurando as fêmeas por algum tempo até que elas eram deixadas inativas e, em algumas vezes, totalmente ou parcialmente enterradas no substrato. Os machos foram maiores que as fêmeas nos pares que acasalaram em todas as espécies estudadas e dois indivíduos (de um total de 23 de C. antillensis, um (de 5 de C. sclopetarius e um (de 3 de C. vittatus, que acasalaram com sucesso fertilizando os ovos das fêmeas, foram identificados como intersexo, com gonóporos tanto maculinos quanto femininos.

  18. Floating marine litter as a raft for drifting voyages for Planes minutus (Crustacea: Decapoda: Grapsidae) and Liocarcinus navigator (Crustacea: Decapoda: Polybiidae).

    Science.gov (United States)

    Tutman, P; Kapiris, K; Kirinčić, M; Pallaoro, A

    2017-07-15

    The Columbus crab Planes minutus and Arch-fronted swimming crab Liocarcinus navigator, within their distribution ranges in the Mediterranean, were found rafted on plastic macro-litter floating on the open south Adriatic. While P. minutus was recorded from inanimate flotsam outside of the Mediterranean, L. navigator is herein reported for the first time on floating marine litter. The role of floating litter as habitat or as a dispersal agent for marine invertebrates has received quite attention however, records of decapod crabs drifting on litter has been relatively sparse. Our results suggests that vast quantities of floating debris, comprised primarily of non-biodegradable plastic polymers, probably will augment natural floating substrates in the marine environment, potentially facilitating the spread of invasive species. The dispersion of rafting crabs through floating debris should be investigated given the high potential ecological risk of invasion by exotic species due to the increase in waste production (ecological risk assessment). Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Structure, molting, and mineralization of the dorsal ossicle complex in the gastric mill of the blue crab, Callinectes sapidus.

    Science.gov (United States)

    Vatcher, Hayley E; Roer, Robert D; Dillaman, Richard M

    2015-11-01

    This study examined the mesocardiac and urocardiac ossicles in the gastric mill of the blue crab to describe its structure, mineralization, and dynamics throughout the molt cycle, and to assess its possible utility in age determination. Morphologically, the mineralized ossicles are similar to the calcified dorsal carapace having a lamellate structure comprised of sheets of chitin/protein fibrils. Staining with acridine orange showed the same arrangement of an epicuticle, exocuticle, and endocuticle. In much of the mesocardiac and urocardiac ossicles, the endocuticle is very reduced, with the exocuticle predominating; the reverse of the dimensions of the exoskeleton. The lamellate structure of the ossicles was confirmed with scanning electron microscopy; however, elemental mapping by energy-dispersive analysis of X-rays revealed that the ossicles are mineralized with calcium phosphate, in contrast to the calcium carbonate biomineral of the exoskeleton. The medial tooth of the urocardiac ossicle is not calcified, but the epicuticle is highly elaborated and impregnated with silica. Histological examination of the ossicles demonstrated that they are molted during ecdysis, so despite the appearance of bands in the mesocardiac ossicle, it is difficult to hypothesize how the bands could represent a record of chronological age. © 2015 Wiley Periodicals, Inc.

  20. Climate Change Effects on Respiration Rates of Blue Crab (Callinectes sapidus) from the Patuxent River, Chesapeake Bay

    Science.gov (United States)

    Nyffeler, A.

    2016-02-01

    A rise in atmospheric CO2 induces a greenhouse effect that also causes ocean temperatures and CO2 levels to rise. These environmental changes may represent an additional energetic cost for blue crabs because they rely on the concentration of CO2 in the water to deposit calcium carbonate in their shells. We conducted a respiration experiment to measure the effect of climate change on crab metabolism. Crabs were collected from the Chesapeake Bay and exposed to different heated and acidified conditions. After crabs had been exposed to the environmental conditions in the chambers for two molts, they were placed in respiration chambers to measure rates of oxygen consumption. Results indicated different trends in respiration rates between the treatments, although the patterns were not statistically significant. Crabs exposed to higher temperatures showed elevated respiration rates, while crabs exposed to high CO2 demonstrated decreased respiration rates. The two factors of climate change (high temperature and high CO2) did not demonstrate the highest respiration rate, but rather the crabs exposed to high temperatures and ambient CO2 showed the highest mean respiration rate. These data suggest that crab metabolism may not change as much as expected due to climate changes.

  1. Bioaccumulation and depuration of metals in blue crabs (Callinectes sapidus Rathbun) from a contaminated and clean estuary

    Energy Technology Data Exchange (ETDEWEB)

    Reichmuth, Jessica M., E-mail: jreichmuth02@gmail.co [Department of Biological Sciences, Rutgers, State University of New Jersey, 195 University Avenue, Newark, NJ 07102 (United States); Weis, Peddrick, E-mail: weis@umdnj.ed [Department of Radiology, UMDNJ-Medical School of New Jersey, Newark, NJ 07101 (United States); Weis, Judith S., E-mail: jweis@andromeda.rutgers.ed [Department of Biological Sciences, Rutgers, State University of New Jersey, 195 University Avenue, Newark, NJ 07102 (United States)

    2010-02-15

    Blue crabs from a contaminated estuary (Hackensack Meadowlands-HM) and a cleaner reference site (Tuckerton-TK) were analyzed for Cr, Cu, Hg, Pb, and Zn in muscle and hepatopancreas. Crabs from each site were taken into the laboratory and fed food from the other site, or in another experiment, transplanted to the other site for eight weeks. All crabs were analyzed for metals. Overall, tissue concentrations reflected environmental conditions. Tissue differences were found for Cu, Pb and Zn (all higher in hepatopancreas), and Hg (higher in muscle). HM muscle had more Hg than TK muscle, but did not decrease after transplanting or consuming clean food. HM crabs lost Cu, Pb and Zn in hepatopancreas after being fed clean food or transplanted. TK crabs increased Hg in muscle and Cr and Zn in hepatopancreas after transplantation or being fed contaminated (HM) food. Concentrations were variable, suggesting that blue crabs may not be fully reliable bioindicators of polluted systems. - The accumulation of metals within the muscle and hepatopancreas of blue crabs was highly variable, but often followed environmental concentrations.

  2. Experimental infections of Orchitophrya stellarum (Scuticociliata) in American blue crabs (Callinectes sapidus) and fiddler crabs (Uca minax).

    Science.gov (United States)

    Miller, Terrence L; Small, Hamish J; Peemoeller, Bhae-Jin; Gibbs, David A; Shields, Jeffrey D

    2013-11-01

    Outbreaks of an unidentified ciliate have occurred on several occasions in blue crabs from Chesapeake Bay held during winter months in flow-through systems. The parasite was initially thought to be Mesanophrys chesapeakensis, but molecular analysis identified it as Orchitophyra stellarum, a facultative parasite of sea stars (Asteroidea). We investigated the host-parasite association of O. stellarum in the blue crab host. Crabs were inoculated with the ciliate, or they were held in bath exposures after experimentally induced autotomy of limbs in order to determine potential mechanisms for infection. Crabs inoculated with the ciliate, or exposed to it after experimental autotomy, rapidly developed fatal infections. Crabs that were not experimentally injured, but were exposed to the ciliate, rarely developed infections; thus, indicating that the parasite requires a wound or break in the cuticle as a portal of entry. For comparative purposes, fiddler crabs, Uca minax, were inoculated with the ciliate in a dose-titration experiment. Low doses of the ciliate (10 per crab) were sometimes able to establish infections, but high intensity infections developed quickly at doses over 500 ciliates per crab. Chemotaxis studies were initiated to determine if the ciliate preferentially selected blue crab serum (BCS) over other nutrient sources. Cultures grown on medium with BCS or fetal bovine serum showed some conditioning in their selection for different media, but the outcome in choice experiments indicated that the ciliate was attracted to BCS and not seawater. Our findings indicate that O. stellarum is a facultative parasite of blue crabs. It can cause infections in exposed crabs at 10-15°C, but it requires a portal of entry for successful host invasion, and it may find injured hosts using chemotaxis. Copyright © 2013 Elsevier Inc. All rights reserved.

  3. EFFECTS OF HYPOXIA ON GENE AND PROTEIN EXPRESSION IN THE BLUE CRAB, CALLINECTES SAPIDUS. (R829458C003)

    Science.gov (United States)

    Increases in hypoxic conditions are one of the major factors responsible for declines in estuarine habitat quality, yet to date there are no indicators for recognizing populations of estuarine organisms that are suffering from chronic hypoxic stress. Here we test the hypothesi...

  4. Variations in prevalence of viral, bacterial, and rhizocephalan diseases and parasites of the blue crab (Callinectes sapidus).

    Science.gov (United States)

    Rogers, Holly A; Taylor, Sabrina S; Hawke, John P; Anderson Lively, Julie A

    2015-05-01

    Prevalence of blue crab diseases and parasites has not been consistently monitored in the Gulf of Mexico. To establish current prevalence levels and to more fully understand population dynamics, commercial landing trends, and effects of future natural and anthropogenic disasters on animal health, we measured the prevalence of white spot syndrome virus (WSSV), Loxothylacus texanus, shell disease, and Vibrio spp. in blue crabs collected from Louisiana in 2013 and the beginning of 2014. We used PCR to detect WSSV and L. texanus infections, visual gross diagnosis for L. texanus externae and shell disease, and standard microbiological culture techniques and biochemical testing for Vibrio spp. We found no crabs infected with WSSV or L. texanus. Absence of L. texanus parasitization was expected based on the sampled salinities and the sampling focus on large crabs. Shell disease was present at a level of 54.8% and was most prevalent in the winter and summer and least prevalent in the spring. Vibrio spp. were found in the hemolymph of 22.3% of the crabs and prevalence varied by site, season, and sex. Additionally, three of 39 crabs tested were infected with reo-like virus. Copyright © 2015 Elsevier Inc. All rights reserved.

  5. Occurrence of the Atlantic blue crab Callinectes sapidus Rathbun, 1896 in two Mediterranean coastal habitats: Temporary visitor or permanent resident?

    Science.gov (United States)

    Mancinelli, Giorgio; Carrozzo, Leonardo; Costantini, Maria Letizia; Rossi, Loreto; Marini, Gabriele; Pinna, Maurizio

    2013-12-01

    Coastal habitats worldwide are threatened by invasive alien species (IAS) that can alter community and ecosystem processes. Invasions are of particular concern for the Mediterranean Sea, and IAS-related descriptors of good ecological status have been recently proposed in European reference regulations.

  6. Prevalence of Vibrio parahaemolyticus, and Vibrio vulnificus in blue crabs (Callinectes sapidus), seawater and sediments of the Maryland Coastal Bays.

    Science.gov (United States)

    Rodgers, C; Parveen, S; Chigbu, P; Jacobs, J; Rhodes, M; Harter-Dennis, J

    2014-10-01

    To determine the prevalence of total and pathogenic Vibrio parahaemolyticus (Vp) and V. vulnificus (Vv) in blue crabs, water and sediment from the Maryland Coastal Bays (MCBs), USA. Crab, haemolymph, sediment and seawater samples were collected monthly from four sites in MCBs from February 2012 through October 2012 with environmental parameters recorded. The most-probable-number (MPN) methodology was used to enumerate Vp and Vv with presumptive colonies and the presence of virulence markers confirmed using polymerase chain reaction (PCR). Results indicate that blue crabs contained both Vp and Vv at densities (7·28 and 5·43 log MPN g(-1) , respectively) higher than those reported for bivalves. In addition, markers for clinically relevant strains of both species were detected in over 30% of samples. Haemolymph, sediment and seawater samples were also routinely positive for both species and clinically relevant strains, but generally at lower densities than found in crabs (4·27, 3·28, and 2·39 log MPN g(-1) per ml(-1) Vp, and 4·28, 2·49 and 2·38 log MPN g(-1) per ml(-1) Vv). Blue crabs concentrate Vp and Vv at levels greater than found in water or sediment. While changes in abundance associated with temperature are apparent, there is little evidence to support differences among sampling locations. These results highlight the potential for blue crab related vibriosis and the importance of proper handling, cooking and care of this popular seafood before consumption. © 2014 The Society for Applied Microbiology.

  7. Prevalence, characterization and sources of Listeria monocytogenes in blue crab (Callinectus sapidus) meat and blue crab processing plants.

    Science.gov (United States)

    Pagadala, Sivaranjani; Parveen, Salina; Rippen, Thomas; Luchansky, John B; Call, Jeffrey E; Tamplin, Mark L; Porto-Fett, Anna C S

    2012-09-01

    Seven blue crab processing plants were sampled to determine the prevalence and sources of Listeria spp. and Listeria monocytogenes for two years (2006-2007). A total of 488 raw crabs, 624 cooked crab meat (crab meat) and 624 environmental samples were tested by standard methods. Presumptive Listeria spp. were isolated from 19.5% of raw crabs, 10.8% of crab meat, and 69.5% of environmental samples. L. monocytogenes was isolated from 4.5% of raw crabs, 0.2% of crab meat, and 2.1% of environmental samples. Ninety-seven percent of the isolates were resistant to at least one of the ten antibiotics tested. Eight different serotypes were found among 76 L. monocytogenes isolates tested with the most common being 4b, 1/2b and 1/2a. Automated EcoRI ribotyping differentiated 11 ribotypes among the 106 L. monocytogenes isolates. Based on ribotyping analysis, the distribution of the ribotypes in each processing plant had a unique contamination pattern. A total of 92 ApaI and 88 AscI pulsotypes among the 106 L. monocytogenes isolates were found and distinct pulsotypes were observed in raw crab, crab meat and environmental samples. Ribotypes and serotypes recovered from crab processing plants included subtypes that have been associated with listeriosis cases in other food outbreaks. Our findings suggest that molecular methods may provide critical information about sources of L. monocytogenes in crab processing plants and will augment efforts to improve food safety control strategies such as targeting specific sources of contamination and use of aggressive detergents prior to sanitizing. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Decapod crustaceans in fresh waters of southeastern Bahia, Brazil.

    Science.gov (United States)

    de Almeida, Alexandre Oliveira; Coelho, Petrônio Alves; Luz, Joaldo Rocha; dos Santos, José Tiago Almeida; Ferraz, Neyva Ribeiro

    2008-09-01

    A total of 117 species of freshwater decapod crustaceans are known from Brazil. Knowledge regarding the fauna of Decapoda from inland waters in the state of Bahia, northeast Brazil, is incipient. In spite of its wide territory and rich hydrographic net, only 13 species of limnetic decapods have been reported from that state. The objective of this contribution was to survey decapod crustaceans of some hydrographic basins in southeastern Bahia. The material described herein was obtained in samplings conducted between 1997 and 2005. Voucher specimens were deposited in the carcinological collections of the Museu de Zoologia, Universidade Estadual de Santa Cruz, Ilhéus, Brazil, and Departamento de Oceanografia, Universidade Federal de Pernambuco, Recife, Brazil. A total of 13 species was collected. The carideans were represented by the atyids Atya scabra (Leach, 1815) and Potimirim potimirim (Müller, 1881) and the palaemonids Macrobrachium acanthurus (Wiegmann, 1836), M. amazonicum (Heller, 1862), M. carcinus (Linnaeus, 1758), M. heterochirus (Wiegmann, 1836), M. jelskii (Miers, 1877), M. olfersi (Wiegmann, 1836), and Palaemon (Palaemon) pandaliformis (Stimpson, 1871). The brachyurans were represented by the portunids Callinectes bocourti A. Milne-Edwards, 1879 and C. sapidus Rathbun, 1895, the trichodactylid Trichodactylus fluviatilis Latreille, 1828 and the panopeid Panopeus rugosus A. Milne-Edwards, 1881. Macrobrachium heterochirus represents a new record from Bahia, and M. amazonicum is reported for the first time in southeast Bahia. The occurrence of two extreme different forms of T. fluviatilis was observed. Form A is characterized by the frontal margin of carapace bordered by conspicuous granules, the anterolateral margin provided with developed teeth plus granules, and the posterolateral margin provided with granulation similar to that found on the front. In form B the frontal margin is smooth or has an inconspicuous granulation; the anterolateral margin is

  9. Salmoneus yoyo nov. sp., a peculiar new infaunal shrimp from Lombok, Indonesia (Decapoda, Caridea, Alpheidae).

    Science.gov (United States)

    Anker, Arthur; Firdaus, Muhammad; Pratama, Idham Sumarto

    2014-08-18

    An unusual new species of the alpheid shrimp genus Salmoneus Holthuis, 1955 is described from Sekotong Bay, southwestern Lombok, Indonesia. The holotype and single known specimen of Salmoneus yoyo sp. nov. was collected with a suction pump from a burrow of unknown host, on a seagrass flat partly exposed at low tide. The new species presents three characters on the chelipeds that are unique within Salmoneus: a conspicuous, mesially curved, hook-like process on the distomesial margin of the merus, a row of blunt teeth on the ventromesial margin of the merus, and a series of blunt teeth on the ventromesial margin of the propodus. Salmoneus yoyo sp. nov. also has a characteristic colour pattern consisting of bright red chromatophores occupying most of the carapace surface, except for the frontal and post-frontal areas. 

  10. The curious case of Neotroglocarcinus dawydoffi (Decapoda, Cryptochiridae): unforeseen biogeographic patterns resulting from isolation

    KAUST Repository

    Van Der Meij, Sancia E.T.

    2014-09-09

    © 2014 The Trustees of the Natural History Museum, London. Coral gall crabs form a commonly overlooked component of the associated fauna of shallow-water reef corals and therefore little is known about their ecology and biogeography. This study investigated the biogeography and phylogenetic position of the informal Detocarcini species group within the Cryptochiridae. We used molecular data for two mitochondrial markers (COI and 16S) obtained from gall crabs covering (part of) a wide geographic range: the Red Sea, Malaysia, Indonesia and New Caledonia. Our phylogeny reconstructions portrayed the Detocarcini as paraphyletic within the monophyletic Cryptochiridae. A phylogeographic clustering was noticed in Neotroglocarcinus dawydoffi that was absent in its sister species, N. hongkongensis, and the closely related species Pseudocryptochirus viridis. A Neighbour Network was estimated for the N. dawydoffi dataset to visualize the similarity between sequences from different biogeographic areas, resulting in three groupings: (1) New Caledonia with Lembeh/Ternate (eastern Indonesia), (2) Semporna/Kudat (eastern Malaysia), and (3) Red Sea (Saudi Arabia). Cryptic speciation rather than isolation is discussed and rejected as an alternative explanation for the observed biogeographic pattern.

  11. Population biology of the crab Armases angustipes (Crustacea, Decapoda, Sesarmidae at Brazilian tropical coast

    Directory of Open Access Journals (Sweden)

    Marina de Sá Leitão C. de Araújo

    2014-06-01

    Full Text Available The semi terrestrial crabs are important elements of the fauna of coastal regions. The aim of this study was to analyze the population structure of Armases angustipes (Dana, 1852 at estuaries of the Ariquindá River, considered a non impacted area, and Mamucabas River, considered a few impacted area, on the south coast of state of Pernambuco, Brazil. The species occurred in all months of the year. The number of individuals per month varied, being higher in the months of transition between the seasons. This is probably due to significant seasonal variations of air and burrow temperature and burrow salinity. There was no sexual dimorphism in size of A. angustipes in the mangrove of Ariquindá River, but males were larger than females in the mangrove of Mamucabas River. In both estuaries, the sex ratio did not differ from Mendelian proportion, but showed a deviation for females. The analysis of temporal variation in sex ratio showed significant differences in some months of the year. These variations are due to cyclical events that act distinctly on each sex. In both estuaries, size classes of carapace width were equally represented by both sexes. The ovigerous females of A. angustipes occurred only in some months of the year, especially in summer, in both estuaries. Probably the high phytoplankton productivity observed in summer favors the reproductive activity, since these algae serve as food for the larvae. Specimens of the population of Rio Ariquindá are largest and wider than those of Mamucabas River. This fact, associated with the low abundance of crabs and the lower frequency of ovigerous females observed in Mamucabas River, is an indication that this population may be influenced by the environmental impacts that this estuary has received.

  12. Majoid crabs community (Crustacea: Decapoda from infralittoral rocky/sandy bottom of Anchieta Island, Ubatuba

    Directory of Open Access Journals (Sweden)

    Fernando L. Mantelatto

    2004-06-01

    Full Text Available The aim of the present study was to characterize the composition of Majoidea crabs inhabiting the infralittoral rocky/sandy bottom of Anchieta Island (Ubatuba, from north coast of São Paulo State. Crabs were collected monthly during 1999 and 2000 by using SCUBA and cages. The occurrence of eight species of majoids (Epialtus brasiliensis, Mithraculus forceps, Mithrax braziliensis, Mithrax hispidus, Mithrax tortugae, Pelia rotunda, Pitho lherminieri and Stenorhynchus seticornis was registered and corresponded to 24.2% of total of majid crabs recorded in the coast of São Paulo State. Mithraculus forceps, Mithrax tortugae and Stenorhynchus seticornis were most abundant in samples and constituted populations that belonged to the brachyuran community of the studied area. The taxocoenosis of the area was dominated by M. forceps (57.62%. The occurrence of Mithrax braziliensis in the present study enlarged its south distribution to the São Paulo State.O objetivo do presente estudo foi caracterizar a composição dos caranguejos Majoidea habitantes do infralitoral rochoso/arenoso da Ilha Anchieta (Ubatuba, litoral norte do Estado de São Paulo. Os caranguejos foram coletados mensalmente durante 1999 e 2000, por mergulho autônomo e armadilhas. Foi registrada a ocorrência de oito espécies de caranguejos majóideos (Epialtus brasiliensis, Mithraculus forceps, Mithrax braziliensis, Mithrax hispidus, Mithrax tortugae, Pelia rotunda, Pitho lherminieri e Stenorhynchus seticornis o que corresponde a 24,2% do total de majóideos registrados para o litoral do Estado de São Paulo. Mithraculus forceps, Mithrax tortugae e Stenorhynchus seticornis constituem populações frequentes na comunidade da área de estudo. A taxoeconose da área foi dominada por M. foreceps (57,62%. A ocorrência de Mithrax braziliensis no presente estudo amplia sua distribuição sul para o Estado de São Paulo.

  13. Aquacultural importance of the integumental pore pattern in postlarval whiteleg shrimp Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Jorge Alberto Cabrera-Jiménez

    2006-09-01

    Full Text Available The analysis of integumental pore pattern has identified two intraspecific groups of postlarval shrimp Litopenaeus vannamei (Boone, 1931, a species of which three to five natural populations or groups are expected to be found from Mexico to Peru. Natural distribution and man-made redistributions have not been typified on a population basis in any penaeid, but it is important to do so in this commercially significant group. Important factors to be defined on a geographic population or group basis include differences in domesticity (eg resistance to pathological agents; food conversion indexes and food preferences; growth indices; tolerance to stress of various origins and other attributes useful in aquaculture. This consideration is valid not only for L. vannamei but also for any other shrimp species that has been the object of artificial redistribution. Rev. Biol. Trop. 54 (3: 707-709. Epub 2006 Sept. 29.Mediante el análisis del patrón de poros tegumentarios ha sido posible caracterizar a dos grupos intraespecíficos en dos estadios postlarvales del camarón Litopenaeus vannamei. Se estima que se podrán encontrar entre tres y cinco grupos o poblaciones naturales en el área de distribución natural desde México hasta Perú. En ningún peneido se ha tipificado a los grupos o poblaciones naturales, lo cual es muy importante no solamente en relación con las poblaciones en su medio natural, sino también en relación a las redistribuciones hechas por el ser humano. Esto se debe a las implicaciones acuiculturales y a las diferencias en domesticidad, como la resistencia o sensibilidad a enfermedades virales y a otros agentes patógenos, el factor de conversión y las preferencias alimenticias, el índice de crecimiento, la tolerancia al estrés de diversos orígenes y otros atributos útiles en la acuicultura, todos los cuales no están definidos aún sobre la base de grupos o poblaciones geográficas. Esta consideración es válida no solamente para L. vannamei sino para cualesquier otra especie de peneido que haya sido objeto de redistribución artificial.

  14. Digestive proteinases of red shrimp Pleoticus muelleri (Decapoda, Penaeoidea): partial characterization and relationship with molting.

    Science.gov (United States)

    Fernández Gimenez, A V; García-Carreño, F L; Navarrete del Toro, M A; Fenucci, J L

    2001-10-01

    The present study describes the activity and some characteristics of proteinases in the hepatopancreas of red shrimp Pleoticus muelleri during the different stages of the molting cycle. Proteolytic activity was highest between pH 7.5 and 8. The hepatopancreatic protein content in the premolt stage was higher than in the other stages of the molting cycle (P.05). No significant differences were found in total proteolytic activity in the hepatopancreas when comparing molting stages. The proteolytic activity of the P. muelleri hepatopancreas enzyme preparations is the main responsibility of serine proteinases. TLCK, a trypsin inhibitor, reduced azocasein hydrolysis between 26% (intermolt) and 37% (premolt). TPCK, a chymotrypsin inhibitor, did not decrease hydrolytic activity, except for in postmolt. Low trypsin and chymotrypsin activities were found during intermolt, and increased in postmolt. The electrophoretogram of the enzyme extracts shows 12 bands of activity during intermolt (from 16.6 to 53.1 kDa). Some fractions were not detected in the postmolt and premolt stages. Three low molecular weight trypsin forms (17.4, 19.1 and 20 kDa) were found in all molting stages. One band of chymotrypsin (21.9 kDa) was observed in all molting stages. High molecular mass active bands (66-205 kDa) could not be characterized with inhibitors. Comparison of the protease-specific activity of the hepatopancreas of some species indicated a relationship between digestive enzyme activity and feeding habits of the shrimp. Omnivorous shrimp, such as Penaeus vannamei (syn: Litopenaeus vannamei) and Penaeus monodon, showed higher protease activity than the carnivorous shrimp, Penaeus californiensis (syn: Farfantepenaeus californiensis) and P. muelleri. In fact, the enzymatic activity in the hepatopancreas of P. muelleri showed variations in relation to feeding habit and molting cycle.

  15. Reproductive and morphometric traits of Macrobrachium amazonicum (Decapoda: Palaemonidae from the Pantanal, Brazil, suggests initial speciation

    Directory of Open Access Journals (Sweden)

    Liliam Hayd

    2013-03-01

    Full Text Available The palaemonid shrimp Macrobrachium amazonicum shows an unusually large geographic range (ca. 4 000km across living in coastal, estuarine, and limnic inland habitats of the upper Amazon, Orinoco, and La Plata basins. This raises doubts whether allopatric, ecologically diverse populations belong to the same species. While shrimps from estuarine and Amazonian habitats have been studied in great detail, very little is known about hololimnetic inland populations. In the present study, biological traits related to growth (maximum body size, fresh weight, morphometric relationships and reproduction (sex ratio; occurrence of male morphotypes; minimum sexable size; minimum size of ovigerous females; fecundity; egg size, were studied in M. amazonicum collected from a pond culture and two natural freshwater habitats (Rio Miranda; Lagoa Baiazinha in the Pantanal of Mato Grosso do Sul, Brazil. In total, 2 270 shrimps were examined (603 males; 1 667 females, 157 of these ovigerous. Sex ratio (males:females was at all sampling sites strongly female-biased, ranging from 0.2-0.6. Maximum body size was larger in natural habitats compared to the pond culture, suggesting reduced growth or a shorter life span under artificial mass rearing conditions. Maximum fecundity observed in our material was 676 eggs, reached by the largest female (TL=65mm; Lagoa Baiazinha. A significant difference between slope parameters of linear regressions describing fecundity, either in terms of numbers of eggs laid or of larvae released, in relation to female fresh weight, indicates egg losses. This may be due in part to a 2.4-fold increase in egg volume occurring during the course of embryonic development, while the available space under the abdomen remains limited. Size-weight relationships differed significantly between males and females, indicating sexspecific morphometric differences. Males appear to have a more slender body shape than females, reaching thus lower weight at equal TL. When reproductive and morphometric traits are compared with literature data from estuarine and inland populations living in the Amazon and Orinoco plains, shrimps from the Pantanal show conspicuous peculiarities differing from other populations: (1 maximum body size is far smaller, suggesting shorter longevity; (2 females are consistently larger than males; (3 different male morphotypes are absent; (4 minimum sexable size and (5 minimum size of ovigerous females are smaller. These traits suggest a heterochronic shift (predisplacement of sexual maturation and r-selection. In summary, our data show biologically relevant differences in life-history traits of shrimps from the Pantanal compared to M. amazonicum populations in other regions. All these differences persist also in long-term cultures maintained under constant conditions. Altogether, our data support the hypothesis that M. amazonicum in the Paraná-Paraguay drainage basin has phylogenetically diverged from allopatric populations that are hydrologically separated by continental watersheds, implying an at least incipient vicariant speciation.El camarón palaemónido Macrobrachium amazonicum muestra una distribución geográfica inusualmente grande (aprox. 4 000km de diámetro, vive en las zonas costeras, estuarios y hábitats interiores límnicos del alto Amazonas, Orinoco y las cuencas de La Plata. Esto plantea dudas sobre sí poblaciones alopátricas y ecológicamente diversas pertenecen a la misma especie. Mientras que los camarones de estuarios y de hábitats Amazónicos se han estudiado con gran detalle, se sabe muy poco acerca de las poblaciones holo-limnéticas del interior. En el presente estudio, los rasgos biológicos relacionados con el crecimiento (tamaño máximo del cuerpo, peso fresco, relaciones morfométricas y la reproducción (proporción de sexos; ocurrencia de morfotipos masculinos; tamaño mínimo sexable; tamaño mínimo de las hembras ovígeras; fecundidad; tamaño del huevo, fueron estudiados en M. amazonicum recolectados en un estanque de cultivo y dos hábitats naturales de agua dulce (Río Miranda; Baiazinha Lagoa en el Pantanal de Mato Grosso do Sul, Brasil. En total, 2 270 camarones fueron examinados (603 machos, 1 667 hembras, 157 de estas ovígeras. La proporción de sexos (machos:hembras fue en todos los sitios de muestreo fuertemente sesgada por hembras, desde 0.2 hasta 0.6. El tamaño corporal máximo fue mayor en los hábitats naturales comparado con el estanque de cultivo, sugeriendo un crecimiento reducido o un lapso de vida más corto en condiciones artificiales de crianza masiva. La fecundidad máxima observada en nuestro material fue 676 huevos, alcanzado por la hembra de mayor tamaño (largo total, LT=65mm. Una diferencia significativa entre los parámetros de pendiente de regresiones lineales que describen la fecundidad, comparando el número de huevos puestos o de larvas liberadas, en relación con el peso de la hembra, indican pérdida de huevos. Esto puede ser debido a un aumento de 2.4 veces en el volumen del huevo que ocurre durante el desarrollo embrionario, mientras que el espacio disponible bajo el abdomen sigue siendo limitado. Relaciones de tamaño-peso diferían significativamente entre machos y hembras, lo que indica diferencias morfométricas sexoespecíficas. Los machos parecen tener una forma de cuerpo más delgado que las hembras, alcanzando por lo tanto el peso más bajo en la igualdad de LT. Cuando los rasgos reproductivos y morfométricos son comparados con datos de la literatura de poblaciones estuarinas y poblaciones del interior que viven en el Amazonas y en los llanos del Orinoco, los camarones del Pantanal muestran peculiaridades diferentes: (1 el tamaño máximo del cuerpo es mucho más pequeño, lo que sugiere menor longevidad; (2 las hembras son más grandes que los machos; (3 diferentes morfotipos masculinos están ausentes; (4 el tamaño mínimo sexable y (5 el tamaño mínimo de hembras ovígeras son más pequeños. Estas características sugieren un cambio heterocrónico (pre-desplazamiento de la maduración sexual y la selección-r. En resumen, nuestros datos muestran diferencias biológicamente relevantes en los rasgos de la historia de vida de los camarones del Pantanal comparados con M. amazonicum en otras regiones. Todas estas diferencias persisten también en cultivos a largo plazo mantenidos bajo condiciones constantes. En conjunto, nuestros datos apoyan la hipótesis que M. amazonicum en la desembocadura de la cuenca Paraná-Paraguay ha divergido filogenéticamente de poblaciones alopátricas que están hidrológicamente separadas por cuencas continentales, lo que implica al menos una incipiente especiación vicariante.

  16. Multiple drivers of decline in the global status of freshwater crayfish (Decapoda: Astacidea)

    Science.gov (United States)

    Richman, Nadia I.; Böhm, Monika; Adams, Susan B.; Alvarez, Fernando; Bergey, Elizabeth A.; Bunn, John J. S.; Burnham, Quinton; Cordeiro, Jay; Coughran, Jason; Crandall, Keith A.; Dawkins, Kathryn L.; DiStefano, Robert J.; Doran, Niall E.; Edsman, Lennart; Eversole, Arnold G.; Füreder, Leopold; Furse, James M.; Gherardi, Francesca; Hamr, Premek; Holdich, David M.; Horwitz, Pierre; Johnston, Kerrylyn; Jones, Clive M.; Jones, Julia P. G.; Jones, Robert L.; Jones, Thomas G.; Kawai, Tadashi; Lawler, Susan; López-Mejía, Marilu; Miller, Rebecca M.; Pedraza-Lara, Carlos; Reynolds, Julian D.; Richardson, Alastair M. M.; Schultz, Mark B.; Schuster, Guenter A.; Sibley, Peter J.; Souty-Grosset, Catherine; Taylor, Christopher A.; Thoma, Roger F.; Walls, Jerry; Walsh, Todd S.; Collen, Ben

    2015-01-01

    Rates of biodiversity loss are higher in freshwater ecosystems than in most terrestrial or marine ecosystems, making freshwater conservation a priority. However, prioritization methods are impeded by insufficient knowledge on the distribution and conservation status of freshwater taxa, particularly invertebrates. We evaluated the extinction risk of the world's 590 freshwater crayfish species using the IUCN Categories and Criteria and found 32% of all species are threatened with extinction. The level of extinction risk differed between families, with proportionally more threatened species in the Parastacidae and Astacidae than in the Cambaridae. Four described species were Extinct and 21% were assessed as Data Deficient. There was geographical variation in the dominant threats affecting the main centres of crayfish diversity. The majority of threatened US and Mexican species face threats associated with urban development, pollution, damming and water management. Conversely, the majority of Australian threatened species are affected by climate change, harvesting, agriculture and invasive species. Only a small proportion of crayfish are found within the boundaries of protected areas, suggesting that alternative means of long-term protection will be required. Our study highlights many of the significant challenges yet to come for freshwater biodiversity unless conservation planning shifts from a reactive to proactive approach. PMID:25561679

  17. The first finding of Ostrea cf. puelchana (Bivalvia living as epibiont on Callinectes exasperates (Decapoda

    Directory of Open Access Journals (Sweden)

    Silvio Felipe Barbosa Lima

    2017-05-01

    Full Text Available This paper describes the epibiosis of Ostrea cf. puelchana on Callinectes exasperatus (Gerstaecker, 1856, both collected from the estuary of the Paraíba River, in the state of Paraíba, northeastern Brazil. The basibiont crab was captured using a trap installed in mangrove area at a depth of about 2 meters. The oyster was closely attached to the left side of dorsal carapace covering most of the epibranchial, mesobranchial and metabranchial regions. Possible advantages and disadvantages for both the epibiont and the basibiont are discussed. We believe that young O. cf. puelchana may avoid a variety of potential predators due to the considerable movement capacity of C. exasperatus and may also serve as a small protective shield for the basibiont. However, the oyster, which is a bivalve with an epifaunal lifestyle, is likely to be negatively affected, mainly due to burrowing activity of the crab. This is the first record of epibiosis between bivalves of the genus Ostrea Linnaeus, 1758 and crabs of the genus Callinectes Stimpson, 1860.

  18. An Inventory of Crayfish (Order Decapoda) at the Erie National Wildlife Refuge in Northwestern Pennsylvania

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Whatever their local name may be, crawfish, crawdads, or crabs, crayfish are the longest lived and largest of all freshwater crustaceans and are observed to be quite...

  19. Three species of Crustacea Decapoda Macrura from Southern Brazil, including a new species of Upogebia

    NARCIS (Netherlands)

    Holthuis, L.B.

    1956-01-01

    In June 1954, during an ecological study of the mangroves near the Research Station of the São Paulo University Oceanographic Institute at Cananeia, about 200 km south of Santos, southern Brazil, Dr. S. Gerlach of the Zoological Institute and Museum of the University of Kiel, Germany, collected some

  20. Use of intertidal areas by shrimps (Decapoda in a brazilian Amazon estuary

    Directory of Open Access Journals (Sweden)

    HEBERT A. SAMPAIO

    2014-03-01

    Full Text Available The present work investigated the occupation and the correlation of the shrimp abundance in relation to environmental variables in different habitats (mangroves, salt marshes and rocky outcrops in an Amazon estuary. The collections were made in August and November 2009, at low syzygy tide on Areuá Beach, situated in the Extractive Reserve of Mãe Grande de Curuçá, Pará, Brazil totaling 20 pools. In each environment, we recorded the physical-chemical factors (pH, salinity, and temperature and measured the area (m2 and volume (m3 of every pool through bathymetry. The average pH, salinity, temperature, area and volume of tide pools were 8.75 (± 0.8 standard deviation, 35.45 (± 3, 29.49 °C (± 2.32, 27.41 m2 (± 41.18, and 5.19 m3 (± 8.01, respectively. We caught a total of 4,871 shrimps, distributed in three families and four species: Farfantepenaeus subtilis (98.36% (marine followed by Alpheus pontederiae (0.76% (estuarine, Macrobrachium surinamicum (0.45% and Macrobrachium amazonicum (0.43% predominantly freshwater. The species F. subtilis and A. pontederiae occurred in the three habitats, whereas M. surinamicum occurred in salt marsh and rocky outcrop and M. amazonicum only in marisma. Temperature and pH were the most important environmental descriptors that significantly affected the density and biomass of shrimps.

  1. DNA barcoding of twelve shrimp species (Crustacea: Decapoda from Turkish seas reveals cryptic diversity

    Directory of Open Access Journals (Sweden)

    R. BILGIN

    2014-05-01

    Full Text Available DNA barcoding is a useful tool for the identification and potential discovery of new species. In this study, DNA barcoding was employed by sequencing the mitochondrial cytochrome oxidase subunit I gene (COI to characterize the genetic diversity of 12 shrimp species inhabiting Turkish coastal waters and, when possible, to compare with the genetic data available from different parts of the Mediterranean and eastern Atlantic. This study also comprises the first DNA barcoding study performed in the Turkish Seas using COI. A total of 40 shrimp specimens were collected and analyzed from 9 sites. Generally, the barcoding gap criterion was successful at identifying species; hence COI appeared to be a good marker of choice for DNA barcoding in this group. Out of the 12 species investigated, five were barcoded for the first time. In six species two intraspecific clades were retrieved after the analyses. The results suggest the presence of cryptic diversity in a genetically understudied marine area, Turkish coastal waters, and further investigation in these species using population genetics, taxonomic approaches and nuclear markers is likely to result in designation of new species.

  2. Die Anomuren des Roten Meeres (Crustacea Decapoda: Paguridea, Galatheidea, Hippidea) 1)

    NARCIS (Netherlands)

    Lewinsohn, Ch.

    1969-01-01

    INHALT Einführung................... 4 Technische Bemerkungen................ 9 Systematischer Teil................. 12 Paguridea................... 12 Diogenidae.................. 12 Paguristes.................. 12 Clibanarius.................. 18 Dardanus.................. 26

  3. Distribution, reproduction, and feeding of West African shrimp Plesionika carinata Holthuis, 1951 (Decapoda, Pandalidae)

    Science.gov (United States)

    Burukovsky, R. N.

    2017-09-01

    Plesionika carinata is endemic in the West African tropical biogeographical realm and is distributed from the western Sahara coast (23°35' N) to southern Namibia (29° S). A total of 263 individuals with a total carapace length of 31 to 71 mm have been studied. P. carinata juveniles are pelagic and occur over depths from 340 to 2000 m or more, mainly over the lower part of the continental slope in summer and over the upper part of the continental slope in autumn. Adults are mainly benthic and occur on the edge of the shelf and the upper part of the continental slope (at depths of 300 to 880 m). Ovigerous females have 750 to 3330 eggs 0.45-0.5 × 0.5-0.65 mm in size on pleopods. Plesionika carinata feeds on abundant micronectonic and macroplanktonic crustaceans (euphausiids and pelagic shrimps) at earlier pelagic stages; it is detritophagous/necrophagous and predatory near the bottom when adult.

  4. Thermal biology of the sub-polar–temperate estuarine crab Hemigrapsus crenulatus (Crustacea: Decapoda: Varunidae)

    Science.gov (United States)

    Cumillaf, Juan P.; Blanc, Johnny; Paschke, Kurt; Gebauer, Paulina; Díaz, Fernando; Re, Denisse; Chimal, María E.; Vásquez, Jorge; Rosas, Carlos

    2016-01-01

    ABSTRACT Optimum temperatures can be measured through aerobic scope, preferred temperatures or growth. A complete thermal window, including optimum, transition (Pejus) and critical temperatures (CT), can be described if preferred temperatures and CT are defined. The crustacean Hemigrapsus crenulatus was used as a model species to evaluate the effect of acclimation temperature on: (i) thermal preference and width of thermal window, (ii) respiratory metabolism, and (iii) haemolymph proteins. Dependant on acclimation temperature, preferred temperature was between 11.8°C and 25.2°C while CT was found between a minimum of 2.7°C (CTmin) and a maximum of 35.9°C (CTmax). These data and data from tropical and temperate crustaceans were compared to examine the association between environmental temperature and thermal tolerance. Temperate species have a CTmax limit around 35°C that corresponded with the low CTmax limit of tropical species (34–36°C). Tropical species showed a CTmin limit around 9°C similar to the maximum CTmin of temperate species (5–6°C). The maximum CTmin of deep sea species that occur in cold environments (2.5°C) matched the low CTmin values (3.2°C) of temperate species. Results also indicate that the energy required to activate the enzyme complex (Ei) involved in respiratory metabolism of ectotherms changes along the latitudinal gradient of temperature. PMID:26879464

  5. Carideorum catalogus: the recent species of the dendrobranchiate, stenopodidean, procarididean and caridean shrimps (Crustacea: Decapoda)

    NARCIS (Netherlands)

    De Grave, S.; Fransen, C.H.J.M.

    2011-01-01

    A checklist of recent species of dendrobranchiate, stenopodidean, procarididean and caridean shrimps including synonyms and type localities. Also listed are unavailable names, larval names, nomina dubia and nomina nuda. A complete list of references to original descriptions of taxa listed is

  6. Growth of hatchery raised banana shrimp Penaeus merguiensis (de Man) (Crustacea: Decapoda) juveniles under different salinity

    Digital Repository Service at National Institute of Oceanography (India)

    Saldanha, C.M.; Achuthankutty, C.T.

    occur along the west coast of India during the summer season. It is suggested that this species may be considered for the summer crop along the west coast of India when the salinity in the ponds goes generally higher and the broodstock is also available... in good numbers. A crop rotation of P. monodon with A merguiensis is also recommended as this would reduce pressure on the wild broodstock of the former and may also reduce the chances of recurrence of whitespot baculo virus infection prevalent...

  7. Confirmation of the presence of Janicea antiguensis (Chace, 1972 (Decapoda: Barbouriidae in northeastern and eastern Brazil

    Directory of Open Access Journals (Sweden)

    Bruno W. Giraldes

    Full Text Available The barbouriid shrimp Janicea antiguesis (Chace, 1972 is reported from Porto de Galinhas and Tamandaré in Pernambuco and Guarapari in Espírito Santo, Brazil. These records confirm the presence of J. antiguensis in northeastern and eastern Brazil, considerably extending its Brazilian range from Fernando de Noronha to southern Espírito Santo and also representing the first record of this species from mainland coastal reefs. In Brazil, J. antiguensis occurs in or near marine reef caves, typically at depths between 5 and 15 m, and can be most easily observed while scuba diving at night. Colour photographs of J. antiguensis from various Atlantic localities are provided and its colour pattern is compared to that of the closely related Indo-West Pacific barbouriid shrimp, Parhippolyte misticia (Clark, 1989. Some in situ observations are provided for the Porto de Galinhas population of J. antiguensis. Multiple observations of pairs with both individuals brooding embryos at different developmental stages suggest protandric simultaneous hermaphroditism in J. antiguensis.

  8. Pleonal muscle development in the shrimp Penaeus (Litopenaeus) vannamei (Crustacea: Malacostraca: Decapoda: Dendrobranchiata).

    Science.gov (United States)

    Hertzler, Philip L; Freas, William R

    2009-05-01

    Penaeoidean shrimp pleonal muscle is a valuable economic resource worldwide, but little is known of its development during larval stages. The development of pleonal muscle in Penaeus (Litopenaeus) vannamei was studied by rhodamine-phalloidin staining and laser-scanning confocal microscopy. Dorsal pleonal muscle was first evident at the protozoea I stage while ventral pleonal muscle was present by the protozoea II stage. Identifiable ventral pleonal muscles were evident by the protozoea III stage and all ventral muscle types were present in the mysis I. The tail flex response began at the mysis stage and growth of existing pleonal muscles continued. The pleopods formed during the mysis stages, with coxal and basis muscles developed by mysis III. The pleopods became functional beginning with the first post-larval stage. We conclude that the pleonal muscle pattern of P. vannamei larvae is similar to that of adult Penaeus setiferus, and that homologous muscles are present. The major formation of dorsal pleonal muscles occurs during the protozoea II stage, while significant development of ventral pleonal muscles occurs during the protozoea III stage.

  9. Cleavage and gastrulation of the dendrobranchiate shrimp Penaeus monodon (Crustacea, Malacostraca, Decapoda).

    Science.gov (United States)

    Biffis, Caterina; Alwes, Frederike; Scholtz, Gerhard

    2009-11-01

    The cleavage pattern of the black tiger shrimp Penaeus monodon was analyzed from the first division until gastrulation. Observations were based on microscopy combined with the use of fluorescent dyes, histological techniques, and computer based three-dimensional reconstructions. Early cleavage is holoblastic and follows a stereotypic pattern, which largely corresponds to what is known from other dendrobranchiate decapods. However, for the first time in this group, we report the presence of an intracellular structure throughout early development. This intracellular body (icb) marks the lineage of one of the two enlarged and division-delayed mesendoderm cells that initiate gastrulation. The identity of the icb as a natural marker and putative determinant of the germ line and its implications on the establishment of the body axes are discussed. The icb as a landmark reveals that the same stereotypic cell division pattern can lead to different fates of individual cells. Hence, the results of this study permit an additional approach to study the relation between cell lineage pattern and the identity of cell lineages.

  10. Synalpheus pinkfloydi sp. nov., a new pistol shrimp from the tropical eastern Pacific (Decapoda: Alpheidae).

    Science.gov (United States)

    Anker, Arthur; Hultgren, Kristin M; Grave, Sammy DE

    2017-04-12

    A new, conspicuously coloured species of the alpheid genus Synalpheus Spence Bate, 1888, is described based on material collected on the Pacific coast of Panama. Synalpheus pinkfloydi sp. nov. is closely related to the western Atlantic S. antillensis Coutière, 1909, the two taxa being transisthmian, cryptic sister species. Both species are characterised by the distal areas of their major and minor chelae coloured in an intense, almost glowing pink-red. The morphological differences between S. pinkfloydi sp. nov. and S. antillensis Coutière, 1909 are subtle, being limited to the slightly different proportions of the merus of both chelipeds, distodorsal armature of the major cheliped merus, relative length of the antennal scaphocerite, and body size. However, they are genetically different with a 10.2% sequence divergence in COI. Based on molecular clock estimates, these transisthmian taxa diverged around 6.8-7.8 mya, i.e. well before the final closure of the Isthmus of Panama 2.5-3 mya.

  11. From the Palaeontological Collection of the Universalmuseum Joanneum - The Cenozoic Decapod Crustaceans (Crustacea: Malacostraca: Decapoda).

    Science.gov (United States)

    Hyžný, Matúš; Gross, Martin

    2016-01-01

    Cenozoic decapod crustaceans housed in the collections of the Universalmuseum Joanneum (Graz, Austria) are reviewed. Previous descriptions, geographic and stratigraphic provenance and collection history are discussed. Altogether 72 specimens are figured, including five holotypes. Taxonomic affinity of previously unpublished material is addressed. Gebiacantha sp. from the middle Miocene of Wetzelsdorf is the first fossil record of the genus from the Paratethys.

  12. Muscle development in the marbled crayfish--insights from an emerging model organism (Crustacea, Malacostraca, Decapoda).

    Science.gov (United States)

    Jirikowski, Günther; Kreissl, Sabine; Richter, Stefan; Wolff, Carsten

    2010-09-01

    The development of the crustacean muscular system is still poorly understood. We present a structural analysis of muscle development in an emerging model organism, the marbled crayfish--a representative of the Cambaridae. The development and differentiation of muscle tissue and its relation to the mesoderm-forming cells are described using fluorescent and non-fluorescent imaging tools. We combined immunohistochemical staining for early isoforms of myosin heavy chain with phallotoxin staining of F-actin, which distinguishes early and more differentiated myocytes. We were thus able to identify single muscle precursor cells that serve as starting points for developing muscular units. Our investigations show a significant developmental advance in head appendage muscles and in the posterior end of the longitudinal trunk muscle strands compared to other forming muscle tissues. These findings are considered evolutionary relics of larval developmental features. Furthermore, we document the development of the muscular heart tissue from myogenic precursors and the formation and differentiation of visceral musculature.

  13. A new groundwater-dwelling species of Euryrhynchina from Cameroon (Malacostraca, Decapoda, Euryrhynchidae).

    Science.gov (United States)

    Grave, Sammy DE; Piscart, Christophe; Kayo, Raoul P Tuekam; Anker, Arthur

    2017-04-12

    A new species of the euryrhynchid shrimp genus Euryrhynchina Powell, 1976, E. puteola sp. nov., is described from Cameroon. The new species can be easily distinguished from the only other species in the genus, E. edingtonae Powell, 1976, by the absence of a setiferous lobe on the eye peduncle, the absence of a meral spine on the ambulatory pereiopods, the shape of the scaphocerite tooth, a single spine on the uropodal diaeresis (vs. 2-3), the absence of a podobranch on the second maxilliped, and the absence of appendices internae on the male pleopods 2-5. Although the species was discovered in a phreatic well, it remains unclear if it is a true stygobiont.

  14. Two Species of the Genus Discorsopagurus (Malacostraca: Decapoda: Paguridae New to Korea

    Directory of Open Access Journals (Sweden)

    Jung, Jibom

    2016-04-01

    Full Text Available The genus Discorsopagurus and two species belonging to this genus, Discorsopagurus maclaughlinae and Discorsopagurus tubicola, are herein first reported in Korean waters. Species belonging to Discorsopagurus are easily distinguished from other hermit crabs of the family Paguridae by their telsons that lack indentations and other characteristics such as straight abdomens, fused third and fourth abdominal tergites, and symmetrical uropods. Discorsopagurus maclaughlinae and D. tubicola were found to live in a tube made by a polychaete tubeworm. The two species differ in the shape of the posterior margin of the telson, the length of the pereopod, and the presence of setae on the antennal flagellum. Discorsopagurus tubicola is reported for the second time in the world. In this paper, there will be provided plates and descriptions of the two species.

  15. Intersexuality in the porcellanid crab Pisidia longicornis (Crustacea: Decapoda: Anomura: Porcellanidae

    Directory of Open Access Journals (Sweden)

    Luciane Augusto de Azevedo Ferreira

    2015-04-01

    Full Text Available Intersex specimens of Pisidia longicornis (Linnaeus, 1767 are recorded for the first time and their secondary sexual characters are analyzed. Of 145 specimens of P. longicornis examined in this study, six were identified as intersex individuals. Of these, three presented male secondary sexual characters, well-developed male gonopores and rudimentary female gonopores, whereas the other three had female secondary sexual characters, with female gonopores being more pronounced than the male ones. The present study provides the first record of intersex porcelain crabs.

  16. Cheramus iranicus, a new species of ghost shrimp (Decapoda: Axiidea: Callianassidae) from the Persian Gulf, Iran.

    Science.gov (United States)

    Sepahvand, Vahid; Momtazi, Farzaneh; Tudge, Christopher

    2015-11-10

    A new species of callianassid ghost shrimp is described from the Persian Gulf, Iran. Cheramus iranicus sp. nov. is the first member of Cheramus described from Iranian subtidal waters and can be differentiated by a large cheliped with a ventroproximal spine on the merus and 7 spines on the ventral margin of the ischium, the endopod of the uropod has 4 movable spines on the distal margin and the posterior margin of the telson is strongly bilobed with a prominent medial tooth and two pairs of lateral movable spines. It is compared to other described Cheramus-like species showing a high degree of morphological similarity.

  17. Population genetic history of Aristeus antennatus (Crustacea: Decapoda) in the Western and Central Mediterranean Sea.

    Science.gov (United States)

    Marra, Annamaria; Mona, Stefano; Sà, Rui M; D'Onghia, Gianfranco; Maiorano, Porzia

    2015-01-01

    Aristeus antennatus is an ecologically and economically important deep-water species in the Mediterranean Sea. In this study we investigated the genetic variability of A. antennatus sampled from 10 sampling stations in the Western and Central Mediterranean. By comparing our new samples with available data from the Western area, we aim to identify potential genetic stocks of A. antennatus and to reconstruct its historical demography in the Mediterranean. We analyzed two regions of mitochondrial DNA in 319 individuals, namely COI and 16S. We found two main results: i) the genetic diversity values consistent with previous data within the Mediterranean and the absence of barriers to gene flow within the Mediterranean Sea; ii) a constant long-term effective population size in almost all demes but a strong signature of population expansion in the pooled sample about 50,000 years B.P./ago. We propose two explanation for our results. The first is based on the ecology of A. antennatus. We suggest the existence of a complex meta-population structured into two layers: a deeper-dwelling stock, not affected by fishing, which preserves the pattern of historical demography; and genetically homogeneous demes inhabiting the fishing grounds. The larval dispersal, adult migration and continuous movements of individuals from "virgin" deeper grounds not affected by fishing to upper fishing areas support an effective 'rescue effect' contributing to the recovery of the exploited stocks and explain their genetic homogeneity throughout the Mediterranean Sea. The second is based on the reproduction model of this shrimp: the high variance in offspring production calls for a careful interpretation of the data observed under classical population genetics and Kingman's coalescent. In both cases, management policies for A. antennatus will therefore require careful evaluation of the meta-population dynamics of all stocks in the Mediterranean. In the future, it will be particularly relevant to sample the deepest ones directly.

  18. Deep-sea Mediterranean biology: the case of Aristaeomorpha foliacea (Risso, 1827 (Crustacea: Decapoda: Aristeidae

    Directory of Open Access Journals (Sweden)

    Chrissi-Yianna Politou

    2004-12-01

    Full Text Available Data on the distribution, abundance and biological parameters of the giant red shrimp Aristaeomorphafoliacea were collected during a research survey in deep waters (600-4000 m of the Mediterranean Sea at three locations: the Balearic Sea, the western Ionian and the eastern Ionian in early summer 2001. The shrimp was mainly found in the shallower zone (< 1000 m of the eastern Ionian Sea. Few specimens were caught in the deeper waters of this region, with 1100 m being the lower limit of its distribution. This is the maximum depth reported for the species in the Mediterranean. At the other two locations, the species was scarcely caught and only in the shallowest zone (< 1000 m. In the area and depth zone of high abundance, 5 modal groups for females and 3 for males were distinguished using the Bhattacharya method. The recruitment seems to take place at the shallowest stations (600 m. More than 50% of adult females were in advanced maturity stages. The striking abundance differences of the species between the western and eastern locations may be explained by the different exploitation level of the deep waters (almost null in the eastern Ionian Sea and extensive more westwards. However, the distribution and biological characteristics of A.foliacea indicate a higher vulnerability to over-fishing comparing to the other commercial co-occurring deep-water shrimp Aristeusantennatus.

  19. [Reproduction of the pink shrimp Farfantepenaeus notialis (Decapoda: Penaeidae) in the Colombian Caribbean].

    Science.gov (United States)

    Paramo, Jorge; Pérez, Daniel; Wolff, Matthias

    2014-06-01

    The shallow water pink shrimp (Farfantepenaeus notialis) is among the socioeconomically most important resources of the Caribbean. The lack of biological and fishery information is of great concern for the fisheries management authorities. The presented study therefore aimed at the investigation of the reproductive cycle, the size composition and the size at first maturity of this species as a basis for the ordination and management of this resource. The study was conducted from June 2012 to May 2013 off the coast of the Caribbean Sea of Colombia. A total of 5 356 individuals were collected, identified, classified and preserved for their subsequent analysis in the laboratory. Size, weight, sex and gonad stage were recorded for each specimen. Significant differences were found in sex ratio in all months sampled with a clear predominance of females. Mature females were found year-around, but two reproductive peaks were identified during the periods October-December and April-June. The mean catch total length size (MCS) for females and males was 148.00mm and 122.54mm, respectively. The mean size at maturity (LT50%) was 129.34mm for females and 97.77mm for males. MCS was always above LT50% for both sexes. Considering the large reduction in fishing effort in the Colombian Caribbean Sea over the last years, we could expect that the shrimp population is in a rebuilding process or perhaps it may be already restored.

  20. A new record of pasiphaeid shrimp of the genus Pasiphaea (Crustacea: Decapoda: Caridea) in Korean waters

    Science.gov (United States)

    Kim, Jung Nyun; Choi, Jung Hwa; Jo, Hyun-su

    2016-09-01

    A single specimen of the pelagic shrimps, Pasiphaea japonica Omori, 1976 (Pasiphaeidae) collected in the southeastern waters of Korea is described and illustrated. Although this species occurs widely in the Indo-West Pacific including the Japanese coast of the East/Japan Sea and the middle and southern parts of the East China Sea, this is the first record of the species and the genus in Korean waters. The species is distinguished from other congeners by the following combination of characteristics: non-carinate dorsal sixth abdominal somite with a terminal tooth, rudimentary pleurobranch on the eighth thoracic somite, merus of the first pereopod with more than eight spines, and almost entirely transparent white color.

  1. Population genetic history of Aristeus antennatus (Crustacea: Decapoda in the Western and Central Mediterranean Sea.

    Directory of Open Access Journals (Sweden)

    Annamaria Marra

    Full Text Available Aristeus antennatus is an ecologically and economically important deep-water species in the Mediterranean Sea. In this study we investigated the genetic variability of A. antennatus sampled from 10 sampling stations in the Western and Central Mediterranean. By comparing our new samples with available data from the Western area, we aim to identify potential genetic stocks of A. antennatus and to reconstruct its historical demography in the Mediterranean. We analyzed two regions of mitochondrial DNA in 319 individuals, namely COI and 16S. We found two main results: i the genetic diversity values consistent with previous data within the Mediterranean and the absence of barriers to gene flow within the Mediterranean Sea; ii a constant long-term effective population size in almost all demes but a strong signature of population expansion in the pooled sample about 50,000 years B.P./ago. We propose two explanation for our results. The first is based on the ecology of A. antennatus. We suggest the existence of a complex meta-population structured into two layers: a deeper-dwelling stock, not affected by fishing, which preserves the pattern of historical demography; and genetically homogeneous demes inhabiting the fishing grounds. The larval dispersal, adult migration and continuous movements of individuals from "virgin" deeper grounds not affected by fishing to upper fishing areas support an effective 'rescue effect' contributing to the recovery of the exploited stocks and explain their genetic homogeneity throughout the Mediterranean Sea. The second is based on the reproduction model of this shrimp: the high variance in offspring production calls for a careful interpretation of the data observed under classical population genetics and Kingman's coalescent. In both cases, management policies for A. antennatus will therefore require careful evaluation of the meta-population dynamics of all stocks in the Mediterranean. In the future, it will be particularly relevant to sample the deepest ones directly.

  2. Ontogenic study of isozymes in giant tiger prawn, Penaeus monodon (Crustacea: Decapoda)

    Science.gov (United States)

    Tong, Jin-Gou; Ballment, E.; Benzie, J. A. H.

    1996-12-01

    The patterns of isozyme variation throughout the life cycle of the giant tiger prawn, Penaeus monodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH, PGD, and PROT). The zymograms showed little variation in the number of isozymic loci expressed during larval development compared with results reported for other penaeid prawns. There were specific ontogenic patterns at some loci. As larvae developed beyond the embryonic stage, there was a tendency for intensity of staining and complexity of banding patterns to increase.

  3. Stages and other aspects of the embryology of the parthenogenetic Marmorkrebs (Decapoda, Reptantia, Astacida).

    Science.gov (United States)

    Alwes, Frederike; Scholtz, Gerhard

    2006-04-01

    The early development of the parthenogenetic Marmorkrebs (marbled crayfish) is described with respect to external morphology, cell lineage, and segment formation. Due to its parthenogenetic reproduction mode, the question arises whether or not the marbled crayfish is a suitable model organism for developmental approaches. To address this question, we describe several aspects of the embryonic development until hatching. We establish ten stages based on characteristic external changes in the living eggs such as blastoderm formation, gastrulation process, formation and differentiation of the naupliar and post-naupliar segments, limb bud differentiation, and eye differentiation. The study of the post-naupliar cell division patterns, segment formation, and engrailed expression reveals distinct similarities to that of other freshwater crayfish. On this basis, we evaluate the possibility of a generalization of ontogenetic processes in the Marmorkrebs for either freshwater crayfish or other crustacean developmental systems.

  4. First Record of Aliaporcellana and Lissoporcellana (Crustacea: Decapoda: Porcellanidae from Korea

    Directory of Open Access Journals (Sweden)

    Lee, Sanghui

    2015-07-01

    Full Text Available Two porcelain crab species, Aliaporcellana pygmaea (De Man, 1902 and Lissoporcellana nakasonei (Miyake, 1978, are reported here for the first time from Korean waters based on specimens collected from Jeju Island, Korea. The genus Aliaporcellana Nakasone and Miyake, 1969, and Lissoporcellana Haig, 1978 are also reported here for the first time in Korea. Aliaporcellana pygmaea has a wide distribution. The specimen reported here is the most northern record of this species. While, Lissoporcellana nakasonei, associated with anthozoans, was known for distributing range from New Caledonia to southern Japan. As a result, the distribution rage of L. nakasonei is changed to Korea. Now, 12 species of porcelain crabs have been reported in Korean waters.

  5. New record of the genus Phycocaris Kemp, 1916 (Decapoda: Caridea: Hippolytidae) from Hainan Island, China

    Science.gov (United States)

    Gan, Zhibin; Li, Xinzheng

    2017-05-01

    The monotypic genus Phycocaris Kemp, 1916, which was established based on material from the Indian Ocean and previously only known from the type localities, Australia and Japan, is now newly recorded from the South China Sea. A detailed description of Phycocaris simulans Kemp, 1916 based on the specimen collected from Hainan Island is presented. Specific features and the differences between the Indian Ocean and the present material are described.

  6. Thermal biology of the sub-polar–temperate estuarine crab Hemigrapsus crenulatus (Crustacea: Decapoda: Varunidae

    Directory of Open Access Journals (Sweden)

    Juan P. Cumillaf

    2016-03-01

    Full Text Available Optimum temperatures can be measured through aerobic scope, preferred temperatures or growth. A complete thermal window, including optimum, transition (Pejus and critical temperatures (CT, can be described if preferred temperatures and CT are defined. The crustacean Hemigrapsus crenulatus was used as a model species to evaluate the effect of acclimation temperature on: (i thermal preference and width of thermal window, (ii respiratory metabolism, and (iii haemolymph proteins. Dependant on acclimation temperature, preferred temperature was between 11.8°C and 25.2°C while CT was found between a minimum of 2.7°C (CTmin and a maximum of 35.9°C (CTmax. These data and data from tropical and temperate crustaceans were compared to examine the association between environmental temperature and thermal tolerance. Temperate species have a CTmax limit around 35°C that corresponded with the low CTmax limit of tropical species (34–36°C. Tropical species showed a CTmin limit around 9°C similar to the maximum CTmin of temperate species (5–6°C. The maximum CTmin of deep sea species that occur in cold environments (2.5°C matched the low CTmin values (3.2°C of temperate species. Results also indicate that the energy required to activate the enzyme complex (Ei involved in respiratory metabolism of ectotherms changes along the latitudinal gradient of temperature.

  7. Description of laboratory reared first zoea of Callinectes danae Smith (Crustacea, Decapoda, Portunidae

    Directory of Open Access Journals (Sweden)

    Cheruparambil Sankarankutty

    1999-01-01

    Full Text Available A detailed description of laboratory reared first zoea larva of Callinectes danae Smith, 1869 is given. The larvae utilized for this study were reared in vitro in a special incubator. A comparison with larvae of other species is also attempted.

  8. Caridean shrimps (Crustacea, Decapoda) from seagrass habitats in Hansa Bay, Papua New Guinea

    NARCIS (Netherlands)

    Grave, de Sammy

    1999-01-01

    Five species of caridean shrimps collected from seagrass habitats at Hansa Bay, on the northern coastline of Papua New Guinea are reported. Four species are new to the fauna of Papua New Guinea. Morphological details of Nikoides danae, Latreutes pymoeus and L. porcinus are discussed and compared to

  9. On the taxonomy and distribution of Callinectes Stimpson (Crustacea, Decapoda, Portunidae in Rio Grande do Norte

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    C. Sankarankutty

    1991-01-01

    Full Text Available A faunistic survey of the species of Callinectes Stimpson, 1871 carried out in the coastal and estuarine regions of the State of Rio Grande do Norte yielded five species: C. bocourti A. Milne Edwards, 1879; C. danae Smith, 1869; C. exasperatus (Gerstaecker, 1856; C. larvatus Ordway, 1863 and C. ornatus Ordway, 1863. Specimens examined consist of those collected on a monthly basis from three fixed stations within the estuary of Potengi, Natal during a period of two years and those obtained from other localities. Among the species occuring in the region, C. danae is more abundant and has a wider range of tolerance of salinity while C. larvatus is restricted to the marine habitat. Though C. danae sustains important artisan fishery, C. bocourti of large size also contribute to the fishery.

  10. Description of laboratory reared first zoea of Callinectes danae Smith (Crustacea, Decapoda, Portunidae)

    OpenAIRE

    Cheruparambil Sankarankutty; Sung Yun Hong; Kwang Bong Kim

    1999-01-01

    A detailed description of laboratory reared first zoea larva of Callinectes danae Smith, 1869 is given. The larvae utilized for this study were reared in vitro in a special incubator. A comparison with larvae of other species is also attempted.

  11. The complete mitogenome of the red claw crayfish Cherax quadricarinatus (Von Martens, 1868) (Crustacea: Decapoda: Parastacidae).

    Science.gov (United States)

    Gan, Han Ming; Tan, Mun Hua; Austin, Christopher M

    2016-01-01

    The commercial freshwater crayfish Cherax quadricarinatus complete mitochondrial genome was recovered from partial genome sequencing using the MiSeq Personal Sequencer. The mitogenome has 15,869 base pairs consisting of 13 protein-coding genes, 2 ribosomal subunit genes, 22 transfer RNAs, and a non-coding AT-rich region. The base composition of C. quadricarinatus is 32.16% for T, 23.39% for C, 33.26% for A, and 11.19% for G, with an AT bias of 65.42%.

  12. Efecto de la densidad en el crecimiento de juveniles tempranos de Cherax quadricarinatus (Decapoda: Parastacidae

    Directory of Open Access Journals (Sweden)

    M. C. Gallo-García

    2012-01-01

    Full Text Available En un estudio de cuatro semanas, se evaluó el efecto de tres densidades (134, 178, 223 org/ m2 sobre el crecimiento y supervivencia de juveniles tempranos (0.05 ± 0.02 g de Cherax quadricarinatus cultivados en un sistema de recirculación. Se alcanzaron pesos húmedos de 0.62 a 0.64 g, con tallas de 30.59 a 31.11 mm de longitud total y una supervivencia promedio final de 68%, sin diferencias significativas en los parámetros productivos de las diferentes densidades (p> 0.05. Los resultados sugieren la posibilidad de obtener buenos rendimientos durante el primer mes de cultivo a densidades tan altas como 223 org/m2 en un sistema de recirculación.

  13. Structural alterations in the male reproductive system of the freshwater crayfish, Cherax quadricarinatus (Decapoda, Parastacidae).

    Science.gov (United States)

    Bugnot, Ana B; López Greco, Laura S

    2009-10-01

    No diseases affecting reproductive performance have been previously reported in freshwater crayfishes. This study aims to characterise one reproductive system abnormality found in males of Cherax quadricarinatus reared in captivity. Fifteen adult males of C. quadricarinatus (70-110 g) were purchased from San Mateo S.A. farm (Entre Ríos, Argentina) each season during 2007. Macroscopic analysis showed that 26.6% of the animals sacrificed in winter presented brownish distal vasa deferentia. Histological analysis showed different levels of structural abnormality in the epithelium of the vasa deferentia and spermatophore. Granular and hyaline haemocytes were identified within the vasa deferentia but no significant differences were found in the sperm count between normal and brownish vas deferens. Histological analysis of the crayfishes sacrificed in autumn also showed these modifications in 22% of the animals, however, they did not show the brownish colour under macroscopic analysis. The similarities between the male reproductive system syndrome in shrimps and the abnormalities found in C. quadricarinatus are notable. An unspecific response to thermic stress is a possible explanation of these structural alterations.

  14. Comparative ultrastructure of spermatozoa of the redclaw Cherax quadricarinatus and the yabby Cherax destructor (Decapoda, Parastacidae).

    Science.gov (United States)

    Kouba, Antonín; Niksirat, Hamid; Bláha, Martin

    2015-02-01

    Ultrastructure of spermatozoa of redclaw Cherax quadricarinatus and yabby Cherax destructor were described and compared. The acrosome complex and nucleus are located at the anterior and posterior region of the spermatozoon, respectively. The acrosome is a complex vesicle divided into two parts: the main body of the acrosome appears as a dense cup-shaped structure in longitudinal sagittal view, with the subacrosome zone occupying the central area of the vesicle. The acrosome is larger in C. quadricarinatus (width 2.37±0.27μm, length 1.31±0.23μm) than in C. destructor (width 1.80±0.27μm, length 1.01±0.15μm). There was no significant difference in L:W ratios of the studied species. The subacrosome zone in both species consists of two areas of different electron density. The nucleus is substantially decondensed and irregular in shape, with elaborate extended processes. The examined species exhibited a well-conserved structure of crayfish spermatozoon, similar to those of Cherax cainii and Cherax albidus. Small acrosome size, the absence of radial arms, and an extracellular capsule seem to be the morphological features that mostly distinguish Cherax from the Astacidae and Cambaridae. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Effects of androgenic gland ablation on growth and reproductive parameters of Cherax quadricarinatus males (Parastacidae, Decapoda).

    Science.gov (United States)

    Tropea, Carolina; Hermida, Gladys N; López Greco, Laura S

    2011-11-01

    This work investigates the effects of androgenic gland (AG) ablation on the structure of the reproductive system, development of secondary sexual characters and somatic growth in Cherax quadricarinatus males. The AG ablation, which was performed at an early developmental stage (initial weight: 1.85±0.03 g), had no effect on the somatic growth parameters (specific growth rate and growth increment), but it prevented the re-formation of male gonopores and appendices masculinae. However, the red patch differentiation and chelae size were similar to those in control males. All the ablated animals developed a male reproductive system. Testis structure was macroscopically and histologically normal. The distal portion of the vas deferens (DVD) was enlarged in some animals, with histological alterations of the epithelium and the structure of the spermatophore. Results suggest that the higher growth in males than in females may be due to an indirect effect of the AG on energy investment in reproduction rather than to a direct effect of an androgen. This is the first report of a potential action of the AG on the secretory activity of the distal VD and the structural organization of the spermatophore. Although the AG may play a role in the development of male copulatory organs, its association with the red patch development deserves further research. The results obtained in the present study support and complement those from intersexes of the same species. Copyright © 2011 Elsevier Inc. All rights reserved.

  16. Taxonomy, phylogeny, historical biogeography, and historical ecology of the genus Pontonia (Crustacea: Decapoda: Caridea: Palaemonidae)

    NARCIS (Netherlands)

    Fransen, C.H.J.M.

    2002-01-01

    Species of the genus Pontonia Latreille, 1829, are distributed in tropical and subtropical waters around the world, living in association with either molluscan or ascidian hosts. In the present taxonomic revision, Pontonia sensu lato is divided into six genera: Pontonia sensu stricto; Ascidonia gen.

  17. Feeding of the planktonic shrimp Lucifer faroni Borradaile, 1915 (Crustacea: Decapoda in the laboratory

    Directory of Open Access Journals (Sweden)

    Luz Amelia Vega-Pérez

    1996-01-01

    Full Text Available Feeding behavior of juveniles of the planktonic shrimp Lucifer faxoni Borradaile was studied in the laboratory under light and dark conditions. Newly-hatched nauplii and metanauplii of Artemio were used as prey organisms. The fecding raie of L. foxoni was dependent on prey size and prey density, but was not obviously affected by light or dark conditions. The capture of the prey tended to increase with longer exposure time to prey. The maximum ingestion rate was 17.28 and 13.40 nauplii.L. faxoni .d- , in the light and in the dark conditions, respectively.O comportamento alimentar de espécimens jovens de Lucifer faxoni Borradaile, em laboratório, sob condições de luz e escuro, foi estudado utilizando-se como alimento náuplios recém-eclodidos e metanáuplios de Artemio. Neste estudo a taxa de alimentação de L. faxoni foi influenciada pelo tamanho e concentração da presa, bem como pelo tempo de contato com a mesma. A atividade alimentar de L. faxoni foi maior em condições de luz, quando comparado com as condições de escuro. f- taxa máxima de ingestão calculada foi de 17,28 e 13,40 náuplios.L. faxoni .d- para as condições de luz e escuro, respectivamente.

  18. Use of intertidal areas by shrimps (Decapoda) in a Brazilian Amazon estuary.

    Science.gov (United States)

    Sampaio, Hebert A; Martinelli-Lemos, Jussara M

    2014-03-01

    The present work investigated the occupation and the correlation of the shrimp abundance in relation to environmental variables in different habitats (mangroves, salt marshes and rocky outcrops) in an Amazon estuary. The collections were made in August and November 2009, at low syzygy tide on Areuá Beach, situated in the Extractive Reserve of Mãe Grande de Curuçá, Pará, Brazil totaling 20 pools. In each environment, we recorded the physical-chemical factors (pH, salinity, and temperature) and measured the area (m2) and volume (m3) of every pool through bathymetry. The average pH, salinity, temperature, area and volume of tide pools were 8.75 (± 0.8 standard deviation), 35.45 (± 3), 29.49 °C (± 2.32), 27.41 m2 (± 41.18), and 5.19 m3 (± 8.01), respectively. We caught a total of 4,871 shrimps, distributed in three families and four species: Farfantepenaeus subtilis (98.36%) (marine) followed by Alpheus pontederiae (0.76%) (estuarine), Macrobrachium surinamicum (0.45%) and Macrobrachium amazonicum (0.43%) predominantly freshwater. The species F. subtilis and A. pontederiae occurred in the three habitats, whereas M. surinamicum occurred in salt marsh and rocky outcrop and M. amazonicum only in marisma. Temperature and pH were the most important environmental descriptors that significantly affected the density and biomass of shrimps.

  19. Phylogeny and evolutionary patterns in the Dwarf crayfish subfamily (Decapoda: Cambarellinae.

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    Carlos Pedraza-Lara

    Full Text Available The Dwarf crayfish or Cambarellinae, is a morphologically singular subfamily of decapod crustaceans that contains only one genus, Cambarellus. Its intriguing distribution, along the river basins of the Gulf Coast of United States (Gulf Group and into Central México (Mexican Group, has until now lacked of satisfactory explanation. This study provides a comprehensive sampling of most of the extant species of Cambarellus and sheds light on its evolutionary history, systematics and biogeography. We tested the impact of Gulf Group versus Mexican Group geography on rates of cladogenesis using a maximum likelihood framework, testing different models of birth/extinction of lineages. We propose a comprehensive phylogenetic hypothesis for the subfamily based on mitochondrial and nuclear loci (3,833 bp using Bayesian and Maximum Likelihood methods. The phylogenetic structure found two phylogenetic groups associated to the two main geographic components (Gulf Group and Mexican Group and is partially consistent with the historical structure of river basins. The previous hypothesis, which divided the genus into three subgenera based on genitalia morphology was only partially supported (P = 0.047, resulting in a paraphyletic subgenus Pandicambarus. We found at least two cases in which phylogenetic structure failed to recover monophyly of recognized species while detecting several cases of cryptic diversity, corresponding to lineages not assigned to any described species. Cladogenetic patterns in the entire subfamily are better explained by an allopatric model of speciation. Diversification analyses showed similar cladogenesis patterns between both groups and did not significantly differ from the constant rate models. While cladogenesis in the Gulf Group is coincident in time with changes in the sea levels, in the Mexican Group, cladogenesis is congruent with the formation of the Trans-Mexican Volcanic Belt. Our results show how similar allopatric divergence in freshwater organisms can be promoted through diverse vicariant factors.

  20. Biodiversidad marina de Costa Rica: Crustacea: Decapoda (Penaeoidea, Sergestoidea, Stenopodidea, Caridea, Thalassinidea, Palinura del Caribe

    Directory of Open Access Journals (Sweden)

    Rita Vargas

    1999-12-01

    Full Text Available Aquí se informan para la costa Caribe de Costa Rica un total de 30 especies de camarones y langostas. Estas se clasifican en el Suborden Dendrobranchiata (Penaeoidea, 6 spp.; Sergestoidea, 2 spp.; y en el Suborden Pleocyemata (Stenopididea, 2 spp.; Caridea, 15 spp.; Thalassinidea, 2 spp. y Palinura, 3 spp.. Del total de especies, 22 son informadas por primera vez para Costa Rica, y adicionalmente una, informada previamente para la costa Pacífica, se informa para el Caribe. Además, se amplia la distribución geográfica de Pontonia domestica, previamente informada de Carolina del Norte, Golfo de México y Bahamas; y Rhynchocinetes rigens, de Florida, Bahamas, islas Vírgenes, Bermuda, Madeira y Azores, a la costa Caribe de Costa Rica. De 218 especies de Penaeoidea, Sergestoidea, Stenopodidea y Caridea mencionadas por Chace (1972 para el Caribe, solo 25 (11.5% se han encontrado hasta ahora en la costa Caribe de Costa Rica.A total of 30 species of shrimp and lobster are reported from the Caribbean coast of Costa Rica. They belong to the Suborder Dendrobranchiata: Penaeoidea, 6 spp. and Sergestoidea, 2 spp.; and Suborder Pleocyemata: Stenopodidea, 2 spp.; Caridea, 15 spp.; Thalassinidea, 2 spp.; Palinura, 3 spp. Of these, 22 species are reported for the first time for Costa Rica, and one species previously known from the Pacific coast is informed from the Caribbean coast. Distribution ranges are extended for: Pontonia domestica Gibbes, from North Carolina, Gulf of Mexico and Bahamas; and Rhynchocinetes rigens Gordon, from the northern Caribbean, Bermuda and eastern Atlantic, to the Caribbean coast of Costa Rica. Of 218 species of Penaeoidea, Sergestoidea, Caridea and Stenopodidea mentioned for the Caribbean by Chace (1972, only 25 (11.5% have been reported from the Caribbean coast of Costa Rica.

  1. Studies on planktonic decapoda and stomatopoda (Crustacea) from the Arabian Sea

    Digital Repository Service at National Institute of Oceanography (India)

    Paulinose, V.T.; Goswami, S.C.; Nair, V.R.

    and longitudes 66 degrees 00' and 78 degrees 00'E and depths ranging from 25 to 500 m. Only 40 samples out of 93, contained planktonic decapods and stomatopods. Maximum number of larvae (1501/100 m3) was obtained from Station 228 (Ga. Cr. 13), a very shallow...

  2. Is Crangon crangon (L. 1758, Decapoda, Caridea) food limited in the Wadden Sea?

    Science.gov (United States)

    Hufnagl, Marc; Temming, Axel; Dänhardt, Andreas; Perger, Robert

    2010-10-01

    Dry weight condition (dry weight at length), caloric content and RNA•DNA -1-ratio of brown shrimp Crangon crangon were monitored in temperature controlled starvation experiments and field samples. A significant decrease of dry weight and RNA•DNA -1 was observed within the experiments in all length classes (20, 30, 45 and 60 mm) and at all temperatures (9, 12, 17 and 20 °C). The highest weight loss of -1.9%·d -1 occurred at 20 °C. Dry weight condition and RNA·DNA -1 in field samples from the Wadden Sea (2005-2007) peaked in summer and were lowest in winter, while caloric content increased during winter, possibly indicating vitellogenesis. The results of the starvation experiments were used to interpret observed field conditions suggesting that at least 25% of the shrimp population is food-limited. Food limitation was most pronounced in the period November until April, where up to 75% of the population exhibited signs of starvation or food limitation.

  3. Systematic position of Neocrangon communis (Decapoda, Crangonidae) based on the features of larval morphology.

    Science.gov (United States)

    Sedova, Nina; Grigoriev, Sergey

    2014-07-07

    The present article deals with morphological comparison of four species of shrimp larvae, such as Neocrangon communis and Mesocrangon intermedia, Crangon dalli and C. septemspinosa, inhabiting the Okhotsk Sea and north-western part of the Pacific Ocean. Morphological comparison of I-V zoeal stages is discussed. The main morphological differences of the appropriate larval stages are detected. Most features of C. dalli and C. septemspinosa are similar and differ from M. intermedia and N. communis. It is shown that M. intermedia and N. communis more similar species by their origin than it is accepted to think. It is assumed that these two species should be included into one genus-Mesocrangon. The figures of I, III-V zoeal stages are presented.

  4. Pathology and ultrastructure of an intranuclear bacilliform virus (IBV) infecting brown shrimp Crangon crangon (Decapoda: Crangonidae).

    Science.gov (United States)

    Stentiford, G D; Bateman, K; Feist, S W

    2004-03-10

    The brown shrimp Crangon crangon supports an important fishery in Europe (over 25000 t, valued at 80 million euros in 2000). Through the course of histopathological screening of crustaceans from the Clyde estuary, western Scotland, for the biological effect of contaminants, we have discovered a highly prevalent (up to 100%) non-occluded intranuclear bacilliform virus (IBV) infection in the hepatopancreatic tubule epithelia and midgut epithelia of wild C. crangon. This is the first report of an IBV in this family. We have termed this virus Crangon crangon bacilliform virus (CcBV). Histological and ultrastructural observations suggest that this virus is similar to other IBVs previously described from crabs and penaeid shrimps. The nuclei of virus-infected epithelial cells contained an eosinophilic, hypertrophied viroplasm that marginalised the chromatin of the host nucleus. Infected cells were often separated from their neighbouring cells and their nuclei appeared apoptotic. In heavily infected shrimp, apoptotic cells were expelled into the lumen of the hepatopancreatic tubule or the midgut. Following this stage, some hepatopancreatic tubules became degenerate, with remnants of the basement membrane and myoepithelial lining remaining. Transmission electron microscopy of hypertrophic nuclei revealed the presence of rod-shaped and cylindrical, envelope-bound virions. These virions did not form arrays and were not embedded within occlusion bodies, but did appear to be partially occluded in an amorphous matrix that corresponded to a granular viroplasm. The ultrastructure, morphology and size of the nucleocapsid and the complete virion aligns the virus most closely to the IBVs previously reported from other decapod crustaceans. Due to the pathological manifestation of IBV infection in C. crangon, it appears likely that it can act as a population modulator, particularly at sites where infection prevalence is high, such as that observed in the Clyde estuary.

  5. On the reproductive biology of the females of the shrimp Crangon crangon (L.) (Decapoda, Crangonidae)

    Science.gov (United States)

    Burukovsky, R. N.; Ivanov, E. S.

    2015-02-01

    A three-type scheme of the reproductive strategy of the females of the shrimp Crangon crangon is proposed. ( 1) The sub-Arctic strategy: summer spawning, long-lasting from early June to mid-July. Each female spawns only once within this time period. Despite it being the summertime, the egg size falls into the range of the "winter" spawning of the females inhabiting the boreal areas of the geographic range. ( 2) The boreal strategy: the spawning takes place all year round. Two groups of females reproduce: large-size winter-spawning (end of November-early March) laying large "winter" eggs and summer-spawning (late March-early April till July) laying small-size "summer" eggs. ( 3) The subtropical strategy: winter spawning (from November through the end of March-beginning of April). The egg size of this group presumably refers to the size of the "summer" eggs produced by the females inhabiting the boreal areas of the geographical range. Such a reproductive strategy of the females of C. crangon refers to the logic of the "excluded fourth case," i.e., either laying the winter eggs during the summer (sub-Arctic), either laying the summer eggs during the winter (subtropics), or both (boreal areas). There is no place for the forth case.

  6. Shoaling of sergestid shrimp Acetes johni (Crustacea Decapoda: Sergestidae) associated with swarming of gammarid amphipods

    Digital Repository Service at National Institute of Oceanography (India)

    Goswami, S.C.

    Aggregation of sergestid shrimp Acetes johni and gammarid amphipods was observed at a station (22 degrees 07'N; 67 degrees 03'E) in the northern Arabian Sea during September 1991. Shoaling occurred in the surface layer at night resulting in high...

  7. Purification and characterization of a lectin from the white shrimp Litopenaeus setiferus (Crustacea decapoda) hemolymph.

    Science.gov (United States)

    Alpuche, Juan; Pereyra, Ali; Agundis, Concepción; Rosas, Carlos; Pascual, Cristina; Slomianny, Marie-Christine; Vázquez, Lorena; Zenteno, Edgar

    2005-06-20

    A 291-kDa lectin (LsL) was purified from the hemolymph of the white shrimp Litopenaeus setiferus by affinity chromatography on glutaraldehyde-fixed stroma from rabbit erythrocytes. LsL is a heterotetramer of two 80-kDa and two 52-kDa subunits, with no covalently-liked carbohydrate, and mainly composed by aspartic and glutamic acids, glycine and alanine, with relatively lower methionine and cysteine contents. Edman degradation indicated that the NH2-terminal of the 80-kDa subunit is composed DASNAQKQHDVNFLL, whereas the NH2-terminal of the 52-kDa subunit is blocked. The peptide mass fingerprint of LsL was predicted from tryptic peptides from each subunit by MALDI-TOF, and revealed that each subunit showed 23 and 22%, respectively, homology with the hemocyanin precursor from Litopenaeus vannamei. Circular dichroism analysis revealed beta sheet and alpha helix contents of 52.7 and 6.1%, respectively. LsL agglutinate at higher titers guinea pig, murine, and rabbit erythrocytes its activity is divalent cation-dependent. N-acetylated sugars, such as GlcNAc, GalNAc, and NeuAc, were the most effective inhibitors of the LsL hemagglutinating activity. Sialylated O-glycosylated proteins, such as bovine submaxillary gland mucin, human IgA, and fetuin, showed stronger inhibitory activity than sialylated N-glycosylated proteins, such as human orosomucoid, IgG, transferrin, and lactoferrin. Desialylation of erythrocytes or inhibitory glycoproteins abolished their capacity to bind LsL, confirming the relevance of sialic acid in LsL-ligand interactions.

  8. Genetic variability of the Amazon River prawn Macrobrachium amazonicum (Decapoda, Caridea, Palaemonidae)

    NARCIS (Netherlands)

    Vergamini, F.G.; Pileggi, L.G.; Mantelatto, F.L.

    2011-01-01

    The freshwater prawn Macrobrachium amazonicum is widely distributed in South America, and occupies habitats with a wide range of salinities. Several investigations have revealed the existence of wide intraspecific variability among different populations, although the understanding of this

  9. Escape behavior and neuronal responses to looming stimuli in the crab Chasmagnathus granulatus (Decapoda: Grapsidae).

    Science.gov (United States)

    Oliva, Damián; Medan, Violeta; Tomsic, Daniel

    2007-03-01

    Behavioral responses to looming stimuli have been studied in many vertebrate and invertebrate species, but neurons sensitive to looming have been investigated in very few animals. In this paper we introduce a new experimental model using the crab Chasmagnathus granulatus, which allows investigation of the processes of looming detection and escape decision at both the behavioral and neuronal levels. By analyzing the escape response of the crab in a walking simulator device we show that: (i) a robust and reliable escape response can be elicited by computer-generated looming stimuli in all tested animals; (ii) parameters such as distance, speed, timing and directionality of the escape run, are easy to record and quantify precisely in the walking device; (iii) although the magnitude of escape varies between animals and stimulus presentations, the timing of the response is remarkably consistent and does not habituate at 3 min stimulus intervals. We then study the response of neurons from the brain of the crab by means of intracellular recordings in the intact animal and show that: (iv) two subclasses of previously identified movement detector neurons from the lobula (third optic neuropil) exhibit robust and reliable responses to the same looming stimuli that trigger the behavioral response; (v) the neurons respond to the object approach by increasing their rate of firing in a way that closely matches the dynamics of the image expansion. Finally, we compare the neuronal with the behavioral response showing that: (vi) differences in the neuronal responses to looming, receding or laterally moving stimuli closely reflect the behavioral differences to such stimuli; (vii) during looming, the crab starts to run soon after the looming-sensitive neurons begin to increase their firing rate. The increase in the running speed during stimulus approach faithfully follows the increment in the firing rate, until the moment of maximum stimulus expansion. Thereafter, the neurons abruptly stop firing and the animal immediately decelerates its run. The results are discussed in connection with studies of responses to looming stimuli in the locust.

  10. [Growth and reproduction of the shrimp Atya margaritacea (Decapoda: Atyidae) in Rio Presidio, Sinaloa, Mexico].

    Science.gov (United States)

    Sánchez Palacios, Jesús; Alvarez, Rigoberto Beltrán; Ramírez Lozano, Juan Pedro

    2008-06-01

    Some growth and reproduction parameters of the population of the shrimp Atya margaritacea in Presidio River (Sinaloa, NW Mexico) were studied using 542 organisms collected with a Surber net in five sampling locations distributed from mid- to low river. Total lengths and gross weights ranged from 15 to 96 mm and 0.1 to 25.2 g. The male:female ratio was 1.96:1 and males had larger sizes and weights. The relations of total length (Lt) to gross weight (Pt) were Pt = 1.02 x 10(-5) (Lt)32089 for males and Pt = 2.29 x 10(-5) (Lt)3.0159 for females. Growth in males was positively allometric whereas in females it was isometric. The regressions between total lengths and cephalothorax (Lc) were Log Lc = 1.1118 (Log Lt)- 0.6087 for males and Log Lc = 0.9945 (Log Lt)- 0.4321 for females. The relative growth between these body parts was allometrically positive in males and isometric in females; this result indicates a clear sexual dimorphism. Ovigerous females appeared in the rainy season (July to November). The absolute fecundity ranged from 1860 to 22400 eggs in females of 43 to 59 mm in length and 1.9 to 6.0 g in weight. The equations relating the number of eggs to length and weight were Fec = 8.3 x 10(-7) (Lt)5.8053 and Fec = 732 (Pt)1.836 respectively.

  11. Survival of Ucides cordatus (Decapoda: Ocypodidae megalopae during transport under different conditions of density and duration

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    Robson Ventura

    2010-01-01

    Full Text Available Target areas for Ucides cordatus (Linnaeus, 1763 restocking programs are often located far from the laboratory where larval rearing is developed. During translocation, the larvae are submitted to highly stressful conditions due to handling, packing, and transport activities. The aim of the present study was to assess the mortality rates of U. cordatus megalopae caused by different transportation procedures. Megalopae at loading densities of 50, 150, and 300 ind.L-1 were packed in double polyethylene 12 x 25 cm plastic bags with 200 ml of marine water at salinity 30. The bags were filled with oxygen at a proportion of 1:2 parts of water and sealed tightly. The trepidations during transport were simulated by the use of a shaker device (800 vibrations/minute over periods of three and six hours inside a dark container. The survivorship rates of larvae after simulation were compared to those obtained in control groups, which consisted of plastic vials with megalopae at a loading density of 50 ind.L-1 maintained at rest. Immediately after the two transport simulations, there was no significant difference in survivorship between the treatments and the control. However, 24 hours after simulation some of the tested densities resulted in significantly lower survivorships. The results demonstrated that U. cordatus megalopae can tolerate six hours of shaking during transportation, at high densities with minimal mortality.

  12. The male reproductive system of Hippolyte inermis Leach 1815 (Decapoda, Caridea)

    Science.gov (United States)

    Cobos, Vanesa; Díaz, Vanessa; Raso, Jose Enrique García; Manjón-Cabeza, M. E.

    2011-03-01

    The present work completes a series of studies on the biology of the shrimp Hippolyte inermis Leach 1815, where we suggested the species to be gonochoristic. The morphology of the male reproductive system (testes, vasa deferentia, gonopores) and the different stages of male germ cell development are described for the first time in the genus Hippolyte, using TEM, SEM, and histological methods. All males from 1.70 to 3.42 mm in carapace length had active testes and well-developed vasa deferentia. No case of sex reversal could be found.

  13. Insights into the morphology of symbiotic shrimp eyes (Crustacea, Decapoda, Palaemonidae; the effects of habitat demands

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    Nicola C. Dobson

    2016-04-01

    Full Text Available Morphometric differences in the optical morphology of symbiotic palaemonid shrimps can be observed among species symbiotic with different host organisms. Discriminant functional analysis revealed three distinct groups within the species examined. Of these, bivalve symbionts appear to have an eye design that is solely unique to this host-symbiont grouping, a design that spans across multiple genera of phylogenetically unrelated animals. Although some taxonomic effects may be evident, this does not explain the difference and similarities in eye morphology that are seen within these shrimps. Therefore evolutionary pressures from their host environments are having an impact on the optical morphology of their eyes however, as indicated by host-hopping events there ecological adaptations occur post host invasion.

  14. Insights into the morphology of symbiotic shrimp eyes (Crustacea, Decapoda, Palaemonidae); the effects of habitat demands.

    Science.gov (United States)

    Dobson, Nicola C; Johnson, Magnus L; De Grave, Sammy

    2016-01-01

    Morphometric differences in the optical morphology of symbiotic palaemonid shrimps can be observed among species symbiotic with different host organisms. Discriminant functional analysis revealed three distinct groups within the species examined. Of these, bivalve symbionts appear to have an eye design that is solely unique to this host-symbiont grouping, a design that spans across multiple genera of phylogenetically unrelated animals. Although some taxonomic effects may be evident, this does not explain the difference and similarities in eye morphology that are seen within these shrimps. Therefore evolutionary pressures from their host environments are having an impact on the optical morphology of their eyes however, as indicated by host-hopping events there ecological adaptations occur post host invasion.

  15. Redescription of the early larval stages of the pandalid shrimp Chlorotocus crassicornis (Decapoda: Caridea: Pandalidae).

    Science.gov (United States)

    Landeira, Jose M; Jiang, Guo-Chen; Chan, Tin-Yam; Shih, Tung-Wei; Gozález-Gordillo, J Ignacio

    2015-09-07

    The first four larval stages of the pandalid shrimp Chlorotocus crassicornis (A. Costa, 1871) are described and illustrated from laboratory-reared material obtained from ovigerous females collected in the southwestern Spain and south Taiwan. The second to fourth larval stages of this species are reported for the first time to science. Detailed examination of the first larval stages reveals that previous description misidentified some key larval characters which have prevented its identification in plankton samples. It is found that the zoeal morphology of Chlorotocus is not very different from other pandalid larvae, and in fact closely resembles Plesionika and Heterocarpus.

  16. Notes on the occurrence of some poorly known decapoda (Crustacea) in the Southern North Sea

    NARCIS (Netherlands)

    Adema, J.P.H.M.; Creutzberg, F.; Noort, van G.J.

    1982-01-01

    INTRODUCTION Since April 1972 an ecological trawl-survey programme has been undertaken by the Netherlands Institute for Sea Research (NIOZ), Texel, in the southern North Sea with the R. V. "Aurelia". The main object is to obtain information on distribution, density, biomass and fluctuations of

  17. Redescription of two Dicyemennea (Phylum: Dicyemida) from Rossia pacifica (Mollusca: Cephalopoda: Decapoda).

    Science.gov (United States)

    Furuya, Hidetaka

    2007-08-01

    Two species of dicyemid mesozoan are redescribed from Rossia pacifica Berry, 1911, collected off Iwase in Toyama Bay, Honshu, Japan. Dicyemennea brevicephaloides Bogolepova-Dobrokhotova, 1962, is a large species that reaches about 4,000 microm in length. This species attaches to the surface of the branchial heart appendages. The vermiform stages are characterized as having 23 peripheral cells, a disc-shaped calotte, and an axial cell that extends to the propolar cells. An anterior abortive axial cell is absent in vermiform embryos. Infusoriform embryos consist of 35 cells; a single nucleus is present in each urn cell and the refringent bodies are absent. Dicyemennea rossiae Bogolepova-Dobrokhotova, 1962, is a medium species that reaches about 2000 microm in length. This species lives in folds of the renal appendages. The vermiform stages are characterized as having 29-34 peripheral cells, a conical calotte, and an axial cell that extends to the middle of the metapolar cells. An anterior abortive axial cell is present in vermiform embryos. Infusoriform embryos consist of 39 cells; 2 nuclei are present in each urn cell and the refringent bodies are solid.

  18. Benthic decapod crustaceans associated with captures of Munida spp. (Decapoda: Anomura in the Beagle Channel, Argentina

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    Patricia Pérez Barros

    2004-06-01

    Full Text Available Benthic decapod crustacean assemblages in the Beagle Channel are dominated by the galatheid crabs Munidasubrugosa and Munida gregaria. This study deals with the bathymetric and spatial distribution, density and diversity of the assemblage of decapod species that co-occur with Munida spp. in the Beagle Channel. A total of 131 samples were obtained using an epibenthic trawl from February 1999 to December 2000. They were taken monthly from two depth strata separated by the 40 m isobath (range: 4-262 m, and from three different sites: Bahía Lapataia, Ushuaia and Punta Segunda. Sixteen of the 36 recorded species of decapod crustaceans from the Beagle Channel were caught. Munidasubrugosa and M. gregaria contributed 94% to the total decapod material. Of the remaining 6%, Austropandalusgrayi (Caridea was the most abundant species, constituting 43% of the non-galatheid decapod fraction. Some species, namely Paguruscomptus and Eurypodius spp., were characteristic of shallow waters ( 40 m. The average density and diversity of non-galatheid decapods varied with depth and location. Decapod diversity correlated positively with the density of algae and polychaete tubes. The influence of three-dimensional bottom structure on decapod diversity in the Beagle Channel is discussed.

  19. Extended hatching periods in the subantarctic lithodid crabs Lithodes santolla and Paralomis granulosa (Crustacea: Decapoda: Lithodidae)

    Science.gov (United States)

    Thatje, S.; Calcagno, J. A.; Lovrich, G. A.; Sartoris, F. J.; Anger, K.

    2003-06-01

    Temporal pattern of hatching was studied in the subantarctic lithodid crabs Lithodes santolla (Molina) and Paralomis granulosa (Jaquinot) from the Argentine Beagle Channel. In both species, larval hatching occurred in low daily numbers over an extended period of up to several weeks, depending on hatch size. Low daily hatching activity and low oxygen-consumption rates in freshly hatched P. granulosa larvae are discussed as life history adaptations to, and/or physiological constraints by, the environmental conditions of high latitudes.

  20. A New Report of Two Species of Pagurid Hermit Crabs (Crustacea: Decapoda: Anomura from Korea

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    Won Kim

    2014-01-01

    Full Text Available Pagurus undosus and Pagurus parvispina, collected from the East Sea by fishery trap, are newly recorded from Korean waters. Pagurus undosus is distinguished from other hermit crab by its right cheliped slightly elevated medially, and with broad ridge formed by deep depressions mesial and lateral to midline. Pagurus parvispina is distinguished from other hermit crab by its right cheliped covered with large spine and long tufts of setae. These species live in cold water areas and their geographical distribution is extended southwardly by the present study. A specimen of the former species, P. undosus, was found living in a shelter formed by a sponge, similar to that observed in Pagurus pectinatus. Descriptions and figures of these two species are provided in this paper. Currently, 27 species of the genus Pagurus are recorded in Korean fauna.