WorldWideScience

Sample records for sanger fastq file

  1. Generation of artificial FASTQ files to evaluate the performance of next-generation sequencing pipelines.

    Directory of Open Access Journals (Sweden)

    Matthew Frampton

    Full Text Available Pipelines for the analysis of Next-Generation Sequencing (NGS data are generally composed of a set of different publicly available software, configured together in order to map short reads of a genome and call variants. The fidelity of pipelines is variable. We have developed ArtificialFastqGenerator, which takes a reference genome sequence as input and outputs artificial paired-end FASTQ files containing Phred quality scores. Since these artificial FASTQs are derived from the reference genome, it provides a gold-standard for read-alignment and variant-calling, thereby enabling the performance of any NGS pipeline to be evaluated. The user can customise DNA template/read length, the modelling of coverage based on GC content, whether to use real Phred base quality scores taken from existing FASTQ files, and whether to simulate sequencing errors. Detailed coverage and error summary statistics are outputted. Here we describe ArtificialFastqGenerator and illustrate its implementation in evaluating a typical bespoke NGS analysis pipeline under different experimental conditions. ArtificialFastqGenerator was released in January 2012. Source code, example files and binaries are freely available under the terms of the GNU General Public License v3.0. from https://sourceforge.net/projects/artfastqgen/.

  2. GTZ: a fast compression and cloud transmission tool optimized for FASTQ files.

    Science.gov (United States)

    Xing, Yuting; Li, Gen; Wang, Zhenguo; Feng, Bolun; Song, Zhuo; Wu, Chengkun

    2017-12-28

    The dramatic development of DNA sequencing technology is generating real big data, craving for more storage and bandwidth. To speed up data sharing and bring data to computing resource faster and cheaper, it is necessary to develop a compression tool than can support efficient compression and transmission of sequencing data onto the cloud storage. This paper presents GTZ, a compression and transmission tool, optimized for FASTQ files. As a reference-free lossless FASTQ compressor, GTZ treats different lines of FASTQ separately, utilizes adaptive context modelling to estimate their characteristic probabilities, and compresses data blocks with arithmetic coding. GTZ can also be used to compress multiple files or directories at once. Furthermore, as a tool to be used in the cloud computing era, it is capable of saving compressed data locally or transmitting data directly into cloud by choice. We evaluated the performance of GTZ on some diverse FASTQ benchmarks. Results show that in most cases, it outperforms many other tools in terms of the compression ratio, speed and stability. GTZ is a tool that enables efficient lossless FASTQ data compression and simultaneous data transmission onto to cloud. It emerges as a useful tool for NGS data storage and transmission in the cloud environment. GTZ is freely available online at: https://github.com/Genetalks/gtz .

  3. FASTdoop: a versatile and efficient library for the input of FASTA and FASTQ files for MapReduce Hadoop bioinformatics applications.

    Science.gov (United States)

    Ferraro Petrillo, Umberto; Roscigno, Gianluca; Cattaneo, Giuseppe; Giancarlo, Raffaele

    2017-05-15

    MapReduce Hadoop bioinformatics applications require the availability of special-purpose routines to manage the input of sequence files. Unfortunately, the Hadoop framework does not provide any built-in support for the most popular sequence file formats like FASTA or BAM. Moreover, the development of these routines is not easy, both because of the diversity of these formats and the need for managing efficiently sequence datasets that may count up to billions of characters. We present FASTdoop, a generic Hadoop library for the management of FASTA and FASTQ files. We show that, with respect to analogous input management routines that have appeared in the Literature, it offers versatility and efficiency. That is, it can handle collections of reads, with or without quality scores, as well as long genomic sequences while the existing routines concentrate mainly on NGS sequence data. Moreover, in the domain where a comparison is possible, the routines proposed here are faster than the available ones. In conclusion, FASTdoop is a much needed addition to Hadoop-BAM. The software and the datasets are available at http://www.di.unisa.it/FASTdoop/ . umberto.ferraro@uniroma1.it. Supplementary data are available at Bioinformatics online.

  4. Manipulation of FASTQ data with Galaxy.

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    Blankenberg, Daniel; Gordon, Assaf; Von Kuster, Gregory; Coraor, Nathan; Taylor, James; Nekrutenko, Anton

    2010-07-15

    Here, we describe a tool suite that functions on all of the commonly known FASTQ format variants and provides a pipeline for manipulating next generation sequencing data taken from a sequencing machine all the way through the quality filtering steps. This open-source toolset was implemented in Python and has been integrated into the online data analysis platform Galaxy (public web access: http://usegalaxy.org; download: http://getgalaxy.org). Two short movies that highlight the functionality of tools described in this manuscript as well as results from testing components of this tool suite against a set of previously published files are available at http://usegalaxy.org/u/dan/p/fastq

  5. FQC Dashboard: integrates FastQC results into a web-based, interactive, and extensible FASTQ quality control tool

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    Brown, Joseph; Pirrung, Meg; McCue, Lee Ann

    2017-06-09

    FQC is software that facilitates large-scale quality control of FASTQ files by carrying out a QC protocol, parsing results, and aggregating quality metrics within and across experiments into an interactive dashboard. The dashboard utilizes human-readable configuration files to manipulate the pages and tabs, and is extensible with CSV data.

  6. AfterQC: automatic filtering, trimming, error removing and quality control for fastq data.

    Science.gov (United States)

    Chen, Shifu; Huang, Tanxiao; Zhou, Yanqing; Han, Yue; Xu, Mingyan; Gu, Jia

    2017-03-14

    Some applications, especially those clinical applications requiring high accuracy of sequencing data, usually have to face the troubles caused by unavoidable sequencing errors. Several tools have been proposed to profile the sequencing quality, but few of them can quantify or correct the sequencing errors. This unmet requirement motivated us to develop AfterQC, a tool with functions to profile sequencing errors and correct most of them, plus highly automated quality control and data filtering features. Different from most tools, AfterQC analyses the overlapping of paired sequences for pair-end sequencing data. Based on overlapping analysis, AfterQC can detect and cut adapters, and furthermore it gives a novel function to correct wrong bases in the overlapping regions. Another new feature is to detect and visualise sequencing bubbles, which can be commonly found on the flowcell lanes and may raise sequencing errors. Besides normal per cycle quality and base content plotting, AfterQC also provides features like polyX (a long sub-sequence of a same base X) filtering, automatic trimming and K-MER based strand bias profiling. For each single or pair of FastQ files, AfterQC filters out bad reads, detects and eliminates sequencer's bubble effects, trims reads at front and tail, detects the sequencing errors and corrects part of them, and finally outputs clean data and generates HTML reports with interactive figures. AfterQC can run in batch mode with multiprocess support, it can run with a single FastQ file, a single pair of FastQ files (for pair-end sequencing), or a folder for all included FastQ files to be processed automatically. Based on overlapping analysis, AfterQC can estimate the sequencing error rate and profile the error transform distribution. The results of our error profiling tests show that the error distribution is highly platform dependent. Much more than just another new quality control (QC) tool, AfterQC is able to perform quality control, data

  7. FQC Dashboard: integrates FastQC results into a web-based, interactive, and extensible FASTQ quality control tool.

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    Brown, Joseph; Pirrung, Meg; McCue, Lee Ann

    2017-06-09

    FQC is software that facilitates quality control of FASTQ files by carrying out a QC protocol using FastQC, parsing results, and aggregating quality metrics into an interactive dashboard designed to richly summarize individual sequencing runs. The dashboard groups samples in dropdowns for navigation among the data sets, utilizes human-readable configuration files to manipulate the pages and tabs, and is extensible with CSV data. FQC is implemented in Python 3 and Javascript, and is maintained under an MIT license. Documentation and source code is available at: https://github.com/pnnl/fqc . joseph.brown@pnnl.gov.

  8. Introduction of the Python script STRinNGS for analysis of STR regions in FASTQ or BAM files and expansion of the Danish STR sequence database to 11 STRs

    DEFF Research Database (Denmark)

    Friis, Susanne L; Buchard, Anders; Rockenbauer, Eszter

    2016-01-01

    01, TPOX, vWA), and Amelogenin was analysed with STRinNGS. Sequencing uncovered five common SNPs near four STRs and revealed 20 new alleles in the 207 Danes. Three short homopolymers in the D8S1179 flanking regions caused frequent sequencing errors. In 29 of 3726 allele calls (0.8%), sequences...... with homopolymer errors were falsely assigned as true alleles. An in-house developed script in R compensated for these errors by compiling sequence reads that had identical STR sequences and identical nucleotides in the five common SNPs. In the output file from the R script, all SNP-STR haplotype calls were...

  9. MutScan: fast detection and visualization of target mutations by scanning FASTQ data.

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    Chen, Shifu; Huang, Tanxiao; Wen, Tiexiang; Li, Hong; Xu, Mingyan; Gu, Jia

    2018-01-22

    Some types of clinical genetic tests, such as cancer testing using circulating tumor DNA (ctDNA), require sensitive detection of known target mutations. However, conventional next-generation sequencing (NGS) data analysis pipelines typically involve different steps of filtering, which may cause miss-detection of key mutations with low frequencies. Variant validation is also indicated for key mutations detected by bioinformatics pipelines. Typically, this process can be executed using alignment visualization tools such as IGV or GenomeBrowse. However, these tools are too heavy and therefore unsuitable for validating mutations in ultra-deep sequencing data. We developed MutScan to address problems of sensitive detection and efficient validation for target mutations. MutScan involves highly optimized string-searching algorithms, which can scan input FASTQ files to grab all reads that support target mutations. The collected supporting reads for each target mutation will be piled up and visualized using web technologies such as HTML and JavaScript. Algorithms such as rolling hash and bloom filter are applied to accelerate scanning and make MutScan applicable to detect or visualize target mutations in a very fast way. MutScan is a tool for the detection and visualization of target mutations by only scanning FASTQ raw data directly. Compared to conventional pipelines, this offers a very high performance, executing about 20 times faster, and offering maximal sensitivity since it can grab mutations with even one single supporting read. MutScan visualizes detected mutations by generating interactive pile-ups using web technologies. These can serve to validate target mutations, thus avoiding false positives. Furthermore, MutScan can visualize all mutation records in a VCF file to HTML pages for cloud-friendly VCF validation. MutScan is an open source tool available at GitHub: https://github.com/OpenGene/MutScan.

  10. Sanger dideoxy sequencing of DNA.

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    Walker, Sarah E; Lorsch, Jon

    2013-01-01

    While the ease and reduced cost of automated DNA sequencing has largely obviated the need for manual dideoxy sequencing for routine purposes, specific applications require manual DNA sequencing. For instance, in studies of enzymes or proteins that bind or modify DNA, a DNA ladder is often used to map the site at which an enzyme is bound or a modification occurs. In these cases, the Sanger method for dideoxy sequencing provides a rapid and facile method for producing a labeled DNA ladder. Copyright © 2013 Elsevier Inc. All rights reserved.

  11. Light-weight reference-based compression of FASTQ data.

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    Zhang, Yongpeng; Li, Linsen; Yang, Yanli; Yang, Xiao; He, Shan; Zhu, Zexuan

    2015-06-09

    The exponential growth of next generation sequencing (NGS) data has posed big challenges to data storage, management and archive. Data compression is one of the effective solutions, where reference-based compression strategies can typically achieve superior compression ratios compared to the ones not relying on any reference. This paper presents a lossless light-weight reference-based compression algorithm namely LW-FQZip to compress FASTQ data. The three components of any given input, i.e., metadata, short reads and quality score strings, are first parsed into three data streams in which the redundancy information are identified and eliminated independently. Particularly, well-designed incremental and run-length-limited encoding schemes are utilized to compress the metadata and quality score streams, respectively. To handle the short reads, LW-FQZip uses a novel light-weight mapping model to fast map them against external reference sequence(s) and produce concise alignment results for storage. The three processed data streams are then packed together with some general purpose compression algorithms like LZMA. LW-FQZip was evaluated on eight real-world NGS data sets and achieved compression ratios in the range of 0.111-0.201. This is comparable or superior to other state-of-the-art lossless NGS data compression algorithms. LW-FQZip is a program that enables efficient lossless FASTQ data compression. It contributes to the state of art applications for NGS data storage and transmission. LW-FQZip is freely available online at: http://csse.szu.edu.cn/staff/zhuzx/LWFQZip.

  12. Comparison of the Equine Reference Sequence with Its Sanger Source Data and New Illumina Reads.

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    Jovan Rebolledo-Mendez

    between new, high throughput genomic sequence data generated for Twilight and the EquCab2 reference assembly. Most of these represent errors in the assembly, while approximately 10,000 are demonstrated to be contributions from another horse. Other results are presented that include the binary alignment map file of the mapped Sanger reads, a list of variants identified as discrepancies between the source data and resulting reference, and a BED annotation file that lists the regions of the genome whose consensus was likely derived from low coverage alignments.

  13. CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

    National Research Council Canada - National Science Library

    Dehairs, Jonas; Talebi, Ali; Cherifi, Yacine; Swinnen, Johannes V

    2016-01-01

    .... Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform...

  14. CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing.

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    Dehairs, Jonas; Talebi, Ali; Cherifi, Yacine; Swinnen, Johannes V

    2016-07-01

    The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping up to three alleles from a single Sanger sequencing trace, providing a robust and readily accessible platform to directly identify indels and significantly speed up the characterization of clones.

  15. Frederick Sanger, Erwin Chargaff, and the metamorphosis of specificity.

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    Judson, H F

    1993-12-15

    That a transformation of ruling ideas in genetics and biochemistry took place at the dawn of molecular biology, in the late 1940s, is a commonplace; but the nature and components of that transformation are widely misunderstood. The change is often identified with the importation into biology of new styles of thought and new rigor by the many scientists trained in physics or chemistry who came into the nascent field--notably, Max Delbrück, Max Perutz, Francis Crick, John Kendrew, Maurice Wilkins, Rosalind Franklin. Most generally, the change is supposed to be the realization that genes are made not of protein but of nucleic acid--and this change was initiated, of course, by the work of Oswald Avery and his colleagues. These changes are not mutually exclusive, and both were surely important to the genesis of molecular biology. But logically prior to them, more fundamental, was another transformation in ruling preconceptions, one that has been neglected: the revolution in understanding of the chemical structures--the sequences of subunits--of proteins and of nucleic acids which was wrought by the work of Frederick Sanger and of Erwin Chargaff. This was a metamorphosis in the understanding of biochemical specificity, and while it astonished many biochemists it set free the small groups of those who were beginning to call themselves molecular biologists, enabling them to think of the relationship between genes and proteins in entirely new ways.

  16. Implementing a genomic data management system using iRODS in the Wellcome Trust Sanger Institute

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    Sale Kevin

    2011-09-01

    Full Text Available Abstract Background Increasingly large amounts of DNA sequencing data are being generated within the Wellcome Trust Sanger Institute (WTSI. The traditional file system struggles to handle these increasing amounts of sequence data. A good data management system therefore needs to be implemented and integrated into the current WTSI infrastructure. Such a system enables good management of the IT infrastructure of the sequencing pipeline and allows biologists to track their data. Results We have chosen a data grid system, iRODS (Rule-Oriented Data management systems, to act as the data management system for the WTSI. iRODS provides a rule-based system management approach which makes data replication much easier and provides extra data protection. Unlike the metadata provided by traditional file systems, the metadata system of iRODS is comprehensive and allows users to customize their own application level metadata. Users and IT experts in the WTSI can then query the metadata to find and track data. The aim of this paper is to describe how we designed and used (from both system and user viewpoints iRODS as a data management system. Details are given about the problems faced and the solutions found when iRODS was implemented. A simple use case describing how users within the WTSI use iRODS is also introduced. Conclusions iRODS has been implemented and works as the production system for the sequencing pipeline of the WTSI. Both biologists and IT experts can now track and manage data, which could not previously be achieved. This novel approach allows biologists to define their own metadata and query the genomic data using those metadata.

  17. Electrostatic Potential Maps and Natural Bond Orbital Analysis: Visualization and Conceptualization of Reactivity in Sanger's Reagent

    Science.gov (United States)

    Mottishaw, Jeffery D.; Erck, Adam R.; Kramer, Jordan H.; Sun, Haoran; Koppang, Miles

    2015-01-01

    Frederick Sanger's early work on protein sequencing through the use of colorimetric labeling combined with liquid chromatography involves an important nucleophilic aromatic substitution (S[subscript N]Ar) reaction in which the N-terminus of a protein is tagged with Sanger's reagent. Understanding the inherent differences between this S[subscript…

  18. biobambam: tools for read pair collation based algorithms on BAM files

    Science.gov (United States)

    2014-01-01

    Background Sequence alignment data is often ordered by coordinate (id of the reference sequence plus position on the sequence where the fragment was mapped) when stored in BAM files, as this simplifies the extraction of variants between the mapped data and the reference or of variants within the mapped data. In this order paired reads are usually separated in the file, which complicates some other applications like duplicate marking or conversion to the FastQ format which require to access the full information of the pairs. Results In this paper we introduce biobambam, a set of tools based on the efficient collation of alignments in BAM files by read name. The employed collation algorithm avoids time and space consuming sorting of alignments by read name where this is possible without using more than a specified amount of main memory. Using this algorithm tasks like duplicate marking in BAM files and conversion of BAM files to the FastQ format can be performed very efficiently with limited resources. We also make the collation algorithm available in the form of an API for other projects. This API is part of the libmaus package. Conclusions In comparison with previous approaches to problems involving the collation of alignments by read name like the BAM to FastQ or duplication marking utilities our approach can often perform an equivalent task more efficiently in terms of the required main memory and run-time. Our BAM to FastQ conversion is faster than all widely known alternatives including Picard and bamUtil. Our duplicate marking is about as fast as the closest competitor bamUtil for small data sets and faster than all known alternatives on large and complex data sets.

  19. Translating sanger-based routine DNA diagnostics into generic massive parallel ion semiconductor sequencing

    NARCIS (Netherlands)

    Diekstra, A.; Bosgoed, E.A.J.; Rikken, A.; Lier, B. van; Kamsteeg, E.J.; Tychon, M.W.J.; Derks, R.C.; Soest, R.A.; Mensenkamp, A.R.; Scheffer, H.; Neveling, K.; Nelen, M.R.

    2015-01-01

    BACKGROUND: Dideoxy-based chain termination sequencing developed by Sanger is the gold standard sequencing approach and allows clinical diagnostics of disorders with relatively low genetic heterogeneity. Recently, new next generation sequencing (NGS) technologies have found their way into diagnostic

  20. Targeted next-generation sequencing can replace Sanger sequencing in clinical diagnostics

    NARCIS (Netherlands)

    Sikkema-Raddatz, B.; Johansson, L.F.; de Boer, E.N.; Almomani, R.; Boven, L.G.; van den Berg, M.P.; van Spaendonck-Zwarts, K.Y.; van Tintelen, J.P.; Sijmons, R.H.; Jongbloed, J.D.H.; Sinke, R.J.

    Mutation detection through exome sequencing allows simultaneous analysis of all coding sequences of genes. However, it cannot yet replace Sanger sequencing (SS) in diagnostics because of incomplete representation and coverage of exons leading to missing clinically relevant mutations. Targeted

  1. Translating sanger-based routine DNA diagnostics into generic massive parallel ion semiconductor sequencing.

    Science.gov (United States)

    Diekstra, Adinda; Bosgoed, Ermanno; Rikken, Alwin; van Lier, Bart; Kamsteeg, Erik-Jan; Tychon, Marloes; Derks, Ronny C; van Soest, Ronald A; Mensenkamp, Arjen R; Scheffer, Hans; Neveling, Kornelia; Nelen, Marcel R

    2015-01-01

    Dideoxy-based chain termination sequencing developed by Sanger is the gold standard sequencing approach and allows clinical diagnostics of disorders with relatively low genetic heterogeneity. Recently, new next generation sequencing (NGS) technologies have found their way into diagnostic laboratories, enabling the sequencing of large targeted gene panels or exomes. The development of benchtop NGS instruments now allows the analysis of single genes or small gene panels, making these platforms increasingly competitive with Sanger sequencing. We developed a generic automated ion semiconductor sequencing work flow that can be used in a clinical setting and can serve as a substitute for Sanger sequencing. Standard amplicon-based enrichment remained identical to PCR for Sanger sequencing. A novel postenrichment pooling strategy was developed, limiting the number of library preparations and reducing sequencing costs up to 70% compared to Sanger sequencing. A total of 1224 known pathogenic variants were analyzed, yielding an analytical sensitivity of 99.92% and specificity of 99.99%. In a second experiment, a total of 100 patient-derived DNA samples were analyzed using a blind analysis. The results showed an analytical sensitivity of 99.60% and specificity of 99.98%, comparable to Sanger sequencing. Ion semiconductor sequencing can be a first choice mutation scanning technique, independent of the genes analyzed. © 2014 American Association for Clinical Chemistry.

  2. Simple protocol for population (Sanger sequencing for Zika virus genomic regions

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    Gabriela Bastos Cabral

    2017-11-01

    Full Text Available BACKGROUND A number of Zika virus (ZIKV sequences were obtained using Next-generation sequencing (NGS, a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE The aim of this study was to develop a simple and robust Sanger sequencing protocol targeting ZIKV relevant genetic regions, as envelope protein and nonstructural protein 5 (NS5. In addition, phylogenetic analysis of the ZIKV strains obtained using the present protocol and their comparison with previously published NGS sequences were also carried out. METHODS Six Vero cells isolates from serum and one urine sample were available to develop the procedure. Primer sets were designed in order to conduct a nested RT-PCR and a Sanger sequencing protocols. Bayesian analysis was used to infer phylogenetic relationships. FINDINGS Seven complete ZIKV envelope protein (1,571 kb and six partial NS5 (0,798 Kb were obtained using the protocol, with no amplification of NS5 gene from urine sample. Two NS5 sequences presented ambiguities at positions 495 and 196. Nucleotide analysis of a Sanger sequence and consensus sequence of previously NGS study revealed 100% identity. ZIKV strains described here clustered within the Asian lineage. MAIN CONCLUSIONS The present study provided a simple and low-cost Sanger protocol to sequence relevant genes of the ZIKV genome. The identity of Sanger generated sequences with published consensus NGS support the use of Sanger method for ZIKV population studies. The regions evaluated were able to provide robust phylogenetic signals and may be used to conduct molecular epidemiological studies and monitor viral evolution.

  3. Simple protocol for population (Sanger) sequencing for Zika virus genomic regions.

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    Cabral, Gabriela Bastos; Ferreira, João Leandro de Paula; Souza, Renato Pereira de; Cunha, Mariana Sequetin; Luchs, Adriana; Figueiredo, Cristina Adelaide; Brígido, Luís Fernando de Macedo

    2018-01-01

    A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. The aim of this study was to develop a simple and robust Sanger sequencing protocol targeting ZIKV relevant genetic regions, as envelope protein and nonstructural protein 5 (NS5). In addition, phylogenetic analysis of the ZIKV strains obtained using the present protocol and their comparison with previously published NGS sequences were also carried out. Six Vero cells isolates from serum and one urine sample were available to develop the procedure. Primer sets were designed in order to conduct a nested RT-PCR and a Sanger sequencing protocols. Bayesian analysis was used to infer phylogenetic relationships. Seven complete ZIKV envelope protein (1,571 kb) and six partial NS5 (0,798 Kb) were obtained using the protocol, with no amplification of NS5 gene from urine sample. Two NS5 sequences presented ambiguities at positions 495 and 196. Nucleotide analysis of a Sanger sequence and consensus sequence of previously NGS study revealed 100% identity. ZIKV strains described here clustered within the Asian lineage. The present study provided a simple and low-cost Sanger protocol to sequence relevant genes of the ZIKV genome. The identity of Sanger generated sequences with published consensus NGS support the use of Sanger method for ZIKV population studies. The regions evaluated were able to provide robust phylogenetic signals and may be used to conduct molecular epidemiological studies and monitor viral evolution.

  4. GLASS: assisted and standardized assessment of gene variations from Sanger sequence trace data.

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    Pal, Karol; Bystry, Vojtech; Reigl, Tomas; Demko, Martin; Krejci, Adam; Touloumenidou, Tasoula; Stalika, Evangelia; Tichy, Boris; Ghia, Paolo; Stamatopoulos, Kostas; Pospisilova, Sarka; Malcikova, Jitka; Darzentas, Nikos

    2017-12-01

    Sanger sequencing is still being employed for sequence variant detection by many laboratories, especially in a clinical setting. However, chromatogram interpretation often requires manual inspection and in some cases, considerable expertise. We present GLASS, a web-based Sanger sequence trace viewer, editor, aligner and variant caller, built to assist with the assessment of variations in 'curated' or user-provided genes. Critically, it produces a standardized variant output as recommended by the Human Genome Variation Society. GLASS is freely available at http://bat.infspire.org/genomepd/glass/ with source code at https://github.com/infspiredBAT/GLASS. nikos.darzentas@gmail.com or malcikova.jitka@fnbrno.cz. Supplementary data are available at Bioinformatics online.

  5. An effective combination of sanger and next generation sequencing in diagnostics of primary ciliary dyskinesia.

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    Djakow, Jana; Kramná, Lenka; Dušátková, Lenka; Uhlík, Jiří; Pursiheimo, Juha-Pekka; Svobodová, Tamara; Pohunek, Petr; Cinek, Ondřej

    2016-05-01

    Primary ciliary dyskinesia (PCD) is a multigenic autosomal recessive condition affecting respiratory tract and other organs where ciliary motility is required. The extent of its genetic heterogeneity is remarkable. The aim of the study was to develop a cost-effective pipeline for genetic diagnostics using a combination of Sanger and next generation sequencing (NGS). Data and samples of 33 families with 38 affected subjects with PCD diagnosed in childhood were collected over the territory of the Czech Republic. A panel of 18 PCD causative or candidate genes was implemented into an Illumina TruSeq Custom Amplicon NGS assay, and three ancestral mutations in SPAG1 were screened by conventional Sanger sequencing, which was also used for the confirmation of the NGS results and for the analysis of familial segregation. The causative gene was DNAH5 in 11/33 (33%) probands, SPAG1 in 8/33 (24%), and DNAI1, CCDC40, LRRC6 in one family each. If the high proportion of subjects with bi-allelic ancestral mutations in SPAG1 is corroborated in other Caucasian populations, a simple Sanger sequencing test for these three mutations may serve as an effective pre-screening step, being followed by an NGS panel for other, much larger, PCD genes. We present a combination of Sanger sequencing with an NGS panel for known and candidate PCD genes, implemented in a moderate-size national collection of patients. This strategy has proven to be cost-effective, rapid and reliable, and was able to detect the causative gene in two thirds of our PCD patients. © 2015 Wiley Periodicals, Inc.

  6. Comparison of base composition analysis and Sanger sequencing of mitochondrial DNA for four U.S. population groups.

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    Kiesler, Kevin M; Coble, Michael D; Hall, Thomas A; Vallone, Peter M

    2014-01-01

    A set of 711 samples from four U.S. population groups was analyzed using a novel mass spectrometry based method for mitochondrial DNA (mtDNA) base composition profiling. Comparison of the mass spectrometry results with Sanger sequencing derived data yielded a concordance rate of 99.97%. Length heteroplasmy was identified in 46% of samples and point heteroplasmy was observed in 6.6% of samples in the combined mass spectral and Sanger data set. Using discrimination capacity as a metric, Sanger sequencing of the full control region had the highest discriminatory power, followed by the mass spectrometry base composition method, which was more discriminating than Sanger sequencing of just the hypervariable regions. This trend is in agreement with the number of nucleotides covered by each of the three assays. Published by Elsevier Ireland Ltd.

  7. Simplified large-scale Sanger genome sequencing for influenza A/H3N2 virus.

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    Hong Kai Lee

    Full Text Available BACKGROUND: The advent of next-generation sequencing technologies and the resultant lower costs of sequencing have enabled production of massive amounts of data, including the generation of full genome sequences of pathogens. However, the small genome size of the influenza virus arguably justifies the use of the more conventional Sanger sequencing technology which is still currently more readily available in most diagnostic laboratories. RESULTS: We present a simplified Sanger-based genome sequencing method for sequencing the influenza A/H3N2 virus in a large-scale format. The entire genome sequencing was completed with 19 reverse transcription-polymerase chain reactions (RT-PCRs and 39 sequencing reactions. This method was tested on 15 native clinical samples and 15 culture isolates, respectively, collected between 2009 and 2011. The 15 native clinical samples registered quantification cycle values ranging from 21.0 to 30.56, which were equivalent to 2.4×10(3-1.4×10(6 viral copies/µL of RNA extract. All the PCR-amplified products were sequenced directly without PCR product purification. Notably, high quality sequencing data up to 700 bp were generated for all the samples tested. The completed sequence covered 408,810 nucleotides in total, with 13,627 nucleotides per genome, attaining 100% coding completeness. Of all the bases produced, an average of 89.49% were Phred quality value 40 (QV40 bases (representing an accuracy of circa one miscall for every 10,000 bases or higher, and an average of 93.46% were QV30 bases (one miscall every 1000 bases or higher. CONCLUSIONS: This sequencing protocol has been shown to be cost-effective and less labor-intensive in obtaining full influenza genomes. The constant high quality of sequences generated imparts confidence in extending the application of this non-purified amplicon sequencing approach to other gene sequencing assays, with appropriate use of suitably designed primers.

  8. NPM1 mutation analysis in Acute Myeloid Leukemia: Comparison of three techniques Sanger Sequencing, Pyrosequencing, and Real Time PCR.

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    Kumar, Dushyant; Mehta, Anurag; Panigrahi, Manoj Kumar; Nath, Sukanta; Saikia, Kandarpa Kumar

    2017-11-13

    Nucleophosmin-1 (NPM1) mutations have prognostic importance in acute myeloid leukemia (AML) patients with intermediate-risk karyotype at diagnosis. Approximately 30% of newly diagnosed cytogenetically normal AML (CN-AML) patients harbor NPM1 mutation in India. In this study we compared the efficiency of three molecular techniques in detecting NPM1 mutation in perepherial blood and bone marrow samples. In a single centre cohort we analyzed 165 CN-AML bone marrow/peripheral blood for NPM1 mutation analysis. Around 30 % CN-AML presented with the NPM1 mutation. For the detection, three methods were compared: Sanger sequencing, Pyrosequencing, and Real-timePCR. NPM1 exon 12 mutations were observed in 52 (31.51%) of all CN-AML cases. The sensitivity of Sanger sequencing, Pyrosequencing, andReal-time PCR: 80%, 90%, and 95%, whereas specificity was 95%, 100%, 100% respectively. While minimum limit of mutation detection was 20%-30% for Sanger sequencing, 1-5% for Pyrosequencing and 0.1-1% for Real Time PCR. Sequencing method which is reference method has lowest sensitive and sometimes difficult to interpret. Whereas real-time PCR is a highlysensitive method for mutation detection but limited for specific mutation type, While in our study pyrosequencing emerge as best suitable technique for thedetection of NPM1 mutation detection by Pyr analysis on the basis of its easy of interpretation and less time-consuming processes than sanger sequencing.

  9. Sanger sequencing solved a cryptic case of severe alpha₁-antitrypsin deficiency.

    Science.gov (United States)

    Zhan, Shing H; Abboud, Raja T; Jung, Benjamin; Kuchinka, Brian; Ralston, Diana; Casey, Brett; Mattman, Andre

    2012-04-01

    Alpha(1)-antitrypsin deficiency (AATD) is a clinically under-diagnosed genetic disorder that originates from deleterious mutations in the alpha(1)-antitrypsin (AAT) gene, SERPINA1. Severe deficiency is associated with significant pulmonary and hepatic malfunctions. Conventional clinical diagnosis involves the evaluation of serum AAT level and detection of diseased protein isoforms. In this communication, we describe the investigations of a case of severe AATD in which the AAT levels were well below those expected from the MZ phenotype determined by isoelectric focusing for protease inhibitor type (IEF PI-typing). In addition to the traditional diagnostic method that combines the assessment of serum AAT concentration and IEF PI-typing, we investigated the SERPINA1 gene of the proband and participating family members for mutations using Sanger sequencing. We identified a novel mutation (M409T) in the proband, initially missed by the standard diagnostic approach. The novel mutation was present in 4 out of 8 family members who participated in the study. This report illustrates the diagnostic value of incorporating exon sequencing of the AAT gene into the algorithm for evaluating AATD, particularly when the AAT serum level is significantly lower than expected from IEF PI-typing. Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  10. Automated Sanger DNA sequencing with one label in less than four lanes on gel.

    Science.gov (United States)

    Ansorge, W; Voss, H; Wirkner, U; Schwager, C; Stegemann, J; Pepperkok, R; Zimmermann, J; Erfle, H

    1989-01-01

    Novel Sanger dideoxy sequencing with only one fluorescent dye label for the four bases of one clone and sequence determination in two lanes on polyacrylamide gel is presented, loading A greater than G in one lane and T greater than C in the other. Sequencing reactions for the two bases in each lane are carried out in one tube. At present the ratio of ddATP:ddGTP and ddTTP:ddCPT is set to 5:1 in the two tubes. Distinction between the two bases in one lane is done by comparing the different magnitudes of the peaks. This method increases the capacity since more clones may be run simultaneously on one gel, while keeping the reliability and simplicity that comes with the use of only one fluorescent dye for the four bases of one clone. At present about 200 bases are determined with the one-dye two-lane method on the EMBL's automated fluorescent DNA sequencer, using T7 DNA polymerase. The error rate in the deduced sequence is about 1%. The technique is used for the determination of overlaps in mapping projects. In principle, it is possible to determine the sequence with one dye in only one lane on the gel by choosing the proper ddNTP ratios for all four bases, carrying out reactions in one tube and applying the product in one lane, but the error rate for this one-lane method seems too high at present and further improvements in the uniformity of peaks obtainable with the T7 DNA polymerase or other enzymes are required.

  11. Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus

    DEFF Research Database (Denmark)

    Bartels, Mette Damkjaer; Petersen, Andreas; Worning, Peder

    2014-01-01

    spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing of the spa repeat region. At Hvidovre Hospital, Denmark, whole-genome sequencing (WGS) of all MRSA isolates has been performed routinely since January 2013......, and an in-house analysis pipeline determines the spa types. Due to national surveillance, all MRSA isolates are sent to Statens Serum Institut, where the spa type is determined by PCR and Sanger sequencing. The purpose of this study was to evaluate the reliability of the spa types obtained by 150-bp paired......-end Illumina WGS. MRSA isolates from new MRSA patients in 2013 (n = 699) in the capital region of Denmark were included. We found a 97% agreement between spa types obtained by the two methods. All isolates achieved a spa type by both methods. Nineteen isolates differed in spa types by the two methods, in most...

  12. Homozygosity mapping and targeted sanger sequencing reveal genetic defects underlying inherited retinal disease in families from pakistan.

    Directory of Open Access Journals (Sweden)

    Maleeha Maria

    Full Text Available Homozygosity mapping has facilitated the identification of the genetic causes underlying inherited diseases, particularly in consanguineous families with multiple affected individuals. This knowledge has also resulted in a mutation dataset that can be used in a cost and time effective manner to screen frequent population-specific genetic variations associated with diseases such as inherited retinal disease (IRD.We genetically screened 13 families from a cohort of 81 Pakistani IRD families diagnosed with Leber congenital amaurosis (LCA, retinitis pigmentosa (RP, congenital stationary night blindness (CSNB, or cone dystrophy (CD. We employed genome-wide single nucleotide polymorphism (SNP array analysis to identify homozygous regions shared by affected individuals and performed Sanger sequencing of IRD-associated genes located in the sizeable homozygous regions. In addition, based on population specific mutation data we performed targeted Sanger sequencing (TSS of frequent variants in AIPL1, CEP290, CRB1, GUCY2D, LCA5, RPGRIP1 and TULP1, in probands from 28 LCA families.Homozygosity mapping and Sanger sequencing of IRD-associated genes revealed the underlying mutations in 10 families. TSS revealed causative variants in three families. In these 13 families four novel mutations were identified in CNGA1, CNGB1, GUCY2D, and RPGRIP1.Homozygosity mapping and TSS revealed the underlying genetic cause in 13 IRD families, which is useful for genetic counseling as well as therapeutic interventions that are likely to become available in the near future.

  13. Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens.

    Directory of Open Access Journals (Sweden)

    Linxiang Chen

    Full Text Available Conventional Sanger sequencing remains time-consuming and laborious. In this study, we developed a rapid improved sequencing protocol of 16S rRNA for pathogens identification by using a new combination of SYBR Green I real-time PCR and Sanger sequencing with FTA® cards. To compare the sequencing quality of this method with conventional Sanger sequencing, 12 strains, including three kinds of strains (1 reference strain and 3 clinical strains, which were previously identified by biochemical tests, which have 4 Pseudomonas aeruginosa, 4 Staphyloccocus aureus and 4 Escherichia coli, were targeted. Additionally, to validate the sequencing results and bacteria identification, expanded specimens with 90 clinical strains, also comprised of the three kinds of strains which included 30 samples respectively, were performed as just described. The results showed that although statistical differences (P<0.05 were found in sequencing quality between the two methods, their identification results were all correct and consistent. The workload, the time consumption and the cost per batch were respectively light versus heavy, 8 h versus 11 h and $420 versus $400. In the 90 clinical strains, all of the Pseudomonas aeruginosa and Staphyloccocus aureus strains were correctly identified, but only 26.7% of the Escherichia coli strains were recognized as Escherichia coli, while 33.3% as Shigella sonnei and 40% as Shigella dysenteriae. The protocol described here is a rapid, reliable, stable and convenient method for 16S rRNA sequencing, and can be used for Pseudomonas aeruginosa and Staphyloccocus aureus identification, yet it is not completely suitable for discriminating Escherichia coli and Shigella strains.

  14. Poly Peak Parser: Method and software for identification of unknown indels using Sanger Sequencing of PCR products

    Science.gov (United States)

    Hill, Jonathon T.; Demarest, Bradley L.; Bisgrove, Brent W.; Su, Yi-chu; Smith, Megan; Yost, H. Joseph

    2015-01-01

    Background Genome editing techniques, including ZFN, TALEN and CRISPR, have created a need to rapidly screen many F1 individuals to identify carriers of indels and determine the sequences of the mutations. Current techniques require multiple clones of the targeted region to be sequenced for each individual, which is inefficient when many individuals must be analyzed. Direct Sanger sequencing of a PCR amplified region surrounding the target site is efficient, but Sanger sequencing genomes heterozygous for an indel results in a string of “double peaks” due to the mismatched region. Results In order to facilitate indel identification, we developed an online tool called Poly Peak Parser (available at http://yost.genetics.utah.edu/software.php) that is able to separate chromatogram data containing ambiguous base calls into wild-type and mutant allele sequences. This tool allows the nature of the indel to be determined from a single sequencing run per individual performed directly on a PCR product spanning the targeted site, without cloning. Conclusions The method and algorithm described here facilitate rapid identification and sequence characterization of heterozygous mutant carriers generated by genome editing. Although designed for screening F1 individuals, this tool can also be used to identify heterozygous indels in many contexts. PMID:25160973

  15. Poly peak parser: Method and software for identification of unknown indels using sanger sequencing of polymerase chain reaction products.

    Science.gov (United States)

    Hill, Jonathon T; Demarest, Bradley L; Bisgrove, Brent W; Su, Yi-Chu; Smith, Megan; Yost, H Joseph

    2014-12-01

    Genome editing techniques, including ZFN, TALEN, and CRISPR, have created a need to rapidly screen many F1 individuals to identify carriers of indels and determine the sequences of the mutations. Current techniques require multiple clones of the targeted region to be sequenced for each individual, which is inefficient when many individuals must be analyzed. Direct Sanger sequencing of a polymerase chain reaction (PCR) amplified region surrounding the target site is efficient, but Sanger sequencing genomes heterozygous for an indel results in a string of "double peaks" due to the mismatched region. To facilitate indel identification, we developed an online tool called Poly Peak Parser (available at http://yost.genetics.utah.edu/software.php) that is able to separate chromatogram data containing ambiguous base calls into wild-type and mutant allele sequences. This tool allows the nature of the indel to be determined from a single sequencing run per individual performed directly on a PCR product spanning the targeted site, without cloning. The method and algorithm described here facilitate rapid identification and sequence characterization of heterozygous mutant carriers generated by genome editing. Although designed for screening F1 individuals, this tool can also be used to identify heterozygous indels in many contexts. © 2014 The Authors. Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

  16. Comparing whole-genome sequencing with Sanger sequencing for spa typing of methicillin-resistant Staphylococcus aureus.

    Science.gov (United States)

    Bartels, Mette Damkjær; Petersen, Andreas; Worning, Peder; Nielsen, Jesper Boye; Larner-Svensson, Hanna; Johansen, Helle Krogh; Andersen, Leif Percival; Jarløv, Jens Otto; Boye, Kit; Larsen, Anders Rhod; Westh, Henrik

    2014-12-01

    spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing of the spa repeat region. At Hvidovre Hospital, Denmark, whole-genome sequencing (WGS) of all MRSA isolates has been performed routinely since January 2013, and an in-house analysis pipeline determines the spa types. Due to national surveillance, all MRSA isolates are sent to Statens Serum Institut, where the spa type is determined by PCR and Sanger sequencing. The purpose of this study was to evaluate the reliability of the spa types obtained by 150-bp paired-end Illumina WGS. MRSA isolates from new MRSA patients in 2013 (n = 699) in the capital region of Denmark were included. We found a 97% agreement between spa types obtained by the two methods. All isolates achieved a spa type by both methods. Nineteen isolates differed in spa types by the two methods, in most cases due to the lack of 24-bp repeats in the whole-genome-sequenced isolates. These related but incorrect spa types should have no consequence in outbreak investigations, since all epidemiologically linked isolates, regardless of spa type, will be included in the single nucleotide polymorphism (SNP) analysis. This will reveal the close relatedness of the spa types. In conclusion, our data show that WGS is a reliable method to determine the spa type of MRSA. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  17. Comparison of Illumina de novo assembled and Sanger sequenced viral genomes: A case study for RNA viruses recovered from the plant pathogenic fungus Sclerotinia sclerotiorum.

    Science.gov (United States)

    Khalifa, Mahmoud E; Varsani, Arvind; Ganley, Austen R D; Pearson, Michael N

    2016-07-02

    The advent of 'next generation sequencing' (NGS) technologies has led to the discovery of many novel mycoviruses, the majority of which are sufficiently different from previously sequenced viruses that there is no appropriate reference sequence on which to base the sequence assembly. Although many new genome sequences are generated by NGS, confirmation of the sequence by Sanger sequencing is still essential for formal classification by the International Committee for the Taxonomy of Viruses (ICTV), although this is currently under review. To empirically test the validity of de novo assembled mycovirus genomes from dsRNA extracts, we compared the results from Illumina sequencing with those from random cloning plus targeted PCR coupled with Sanger sequencing for viruses from five Sclerotinia sclerotiorum isolates. Through Sanger sequencing we detected nine viral genomes while through Illumina sequencing we detected the same nine viruses plus one additional virus from the same samples. Critically, the Illumina derived sequences share >99.3 % identity to those obtained by cloning and Sanger sequencing. Although, there is scope for errors in de novo assembled viral genomes, our results demonstrate that by maximising the proportion of viral sequence in the data and using sufficiently rigorous quality controls, it is possible to generate de novo genome sequences of comparable accuracy from Illumina sequencing to those obtained by Sanger sequencing. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Sanger DNA-sequencing reactions performed in a solid-phase nanoreactor directly coupled to capillary gel electrophoresis.

    Science.gov (United States)

    Soper, S A; Williams, D C; Xu, Y; Lassiter, S J; Zhang, Y; Ford, S M; Bruch, R C

    1998-10-01

    A miniaturized, solid-phase nanoreactor was developed to prepare Sanger DNA-sequencing ladders which was directly interfaced to a capillary gel electrophoresis system. A biotinylated fragment of the rat brain actin gene (1 kbp) was amplified by PCR and attached to the interior wall of an (aminoalkyl)silane-derivatized fused-silica capillary tube via a biotin/streptavidin/biotin linkage. Coverage of the capillary wall with the biotinylated DNA averaged 77 +/- 10%. Stability of the anchored template under pressure (33 nL/s) and electroosmotic flows (11.3 nL/s) were favorable, requiring rinsing for > 150 h to reduce the surface coverage by only 50%. In addition, the immobilized template was stable toward temperatures required for preparing sequencing ladders, even under cycling conditions. Standard Sanger dideoxynucleotide termination performed in a large-volume (approximately 8 microL) solid-phase reactor using the thermally stable polymerase enzymes Taq and Vent and the polymerases T7 and Bst with off-line slab gel electrophoresis and autoradiographic detection indicated that acceptable fragment generation was achieved only in the case of the thermally stable polymerases. Banding was not apparent for T7 and Bst since all reagents were inserted into the column in a single plug at the beginning of the reaction. A small volume reactor (volume approximately 62 nL) was then used to perform DNA polymerase reactions and was coupled directly to a capillary gel column for separation. The capillary reactor was placed inside a thermocycler to control the temperature during chain extension and was directly connected to the gel column via zero dead volume fused-silica connectors. The complementary DNA fragments generated (C-track only) in the reactor were denatured using heat and directly injected onto the gel-filled capillary for size separation with detection accomplished using near-IR laser-induced fluorescence. Extension and single-base separation resolution of the C

  19. Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis

    Directory of Open Access Journals (Sweden)

    Anwesha Ghosh

    2017-03-01

    Full Text Available Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach which incorporates Sanger sequencing. In this study, structure of bacterioplankton communities was investigated from two stations of Sundarbans mangrove ecoregion using both Sanger and Illumina MiSeq sequencing approaches. The Illumina MiSeq data is available under the BioProject ID PRJNA35180 and Sanger sequencing data under accession numbers KX014101-KX014140 (Stn1 and KX014372-KX014410 (Stn3. Proteobacteria-, Firmicutes- and Bacteroidetes-like sequences retrieved from both approaches appeared to be abundant in the studied ecosystem. The Illumina MiSeq data (2.1 GB provided a deeper insight into the structure of bacterioplankton communities and revealed the presence of bacterial phyla such as Actinobacteria, Cyanobacteria, Tenericutes, Verrucomicrobia which were not recovered based on Sanger sequencing. A comparative analysis of bacterioplankton communities from both stations highlighted the presence of genera that appear in both stations and genera that occur exclusively in either station. However, both the Sanger sequencing and Illumina MiSeq data were coherent at broader taxonomic levels. Pseudomonas, Devosia, Hyphomonas and Erythrobacter-like sequences were the abundant bacterial genera found in the studied ecosystem. Both the sequencing methods showed broad coherence although as expected the Illumina MiSeq data helped identify rarer bacterioplankton groups and also showed the presence of unassigned OTUs indicating possible presence of novel bacterioplankton from the studied mangrove ecosystem.

  20. 454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine KRAS mutation analysis of formalin-fixed, paraffin-embedded samples

    Science.gov (United States)

    Altimari, Annalisa; de Biase, Dario; De Maglio, Giovanna; Gruppioni, Elisa; Capizzi, Elisa; Degiovanni, Alessio; D’Errico, Antonia; Pession, Annalisa; Pizzolitto, Stefano; Fiorentino, Michelangelo; Tallini, Giovanni

    2013-01-01

    Detection of KRAS mutations in archival pathology samples is critical for therapeutic appropriateness of anti-EGFR monoclonal antibodies in colorectal cancer. We compared the sensitivity, specificity, and accuracy of Sanger sequencing, ARMS-Scorpion (TheraScreen®) real-time polymerase chain reaction (PCR), pyrosequencing, chip array hybridization, and 454 next-generation sequencing to assess KRAS codon 12 and 13 mutations in 60 nonconsecutive selected cases of colorectal cancer. Twenty of the 60 cases were detected as wild-type KRAS by all methods with 100% specificity. Among the 40 mutated cases, 13 were discrepant with at least one method. The sensitivity was 85%, 90%, 93%, and 92%, and the accuracy was 90%, 93%, 95%, and 95% for Sanger sequencing, TheraScreen real-time PCR, pyrosequencing, and chip array hybridization, respectively. The main limitation of Sanger sequencing was its low analytical sensitivity, whereas TheraScreen real-time PCR, pyrosequencing, and chip array hybridization showed higher sensitivity but suffered from the limitations of predesigned assays. Concordance between the methods was k = 0.79 for Sanger sequencing and k > 0.85 for the other techniques. Tumor cell enrichment correlated significantly with the abundance of KRAS-mutated deoxyribonucleic acid (DNA), evaluated as ΔCt for TheraScreen real-time PCR (P = 0.03), percentage of mutation for pyrosequencing (P = 0.001), ratio for chip array hybridization (P = 0.003), and percentage of mutation for 454 next-generation sequencing (P = 0.004). Also, 454 next-generation sequencing showed the best cross correlation for quantification of mutation abundance compared with all the other methods (P < 0.001). Our comparison showed the superiority of next-generation sequencing over the other techniques in terms of sensitivity and specificity. Next-generation sequencing will replace Sanger sequencing as the reference technique for diagnostic detection of KRAS mutation in archival tumor tissues. PMID

  1. Diagnosis of Fanconi Anemia: Mutation Analysis by Multiplex Ligation-Dependent Probe Amplification and PCR-Based Sanger Sequencing

    Directory of Open Access Journals (Sweden)

    Johan J. P. Gille

    2012-01-01

    Full Text Available Fanconi anemia (FA is a rare inherited disease characterized by developmental defects, short stature, bone marrow failure, and a high risk of malignancies. FA is heterogeneous: 15 genetic subtypes have been distinguished so far. A clinical diagnosis of FA needs to be confirmed by testing cells for sensitivity to cross-linking agents in a chromosomal breakage test. As a second step, DNA testing can be employed to elucidate the genetic subtype of the patient and to identify the familial mutations. This knowledge allows preimplantation genetic diagnosis (PGD and enables prenatal DNA testing in future pregnancies. Although simultaneous testing of all FA genes by next generation sequencing will be possible in the near future, this technique will not be available immediately for all laboratories. In addition, in populations with strong founder mutations, a limited test using Sanger sequencing and MLPA will be a cost-effective alternative. We describe a strategy and optimized conditions for the screening of FANCA, FANCB, FANCC, FANCE, FANCF, and FANCG and present the results obtained in a cohort of 54 patients referred to our diagnostic service since 2008. In addition, the follow up with respect to genetic counseling and carrier screening in the families is discussed.

  2. Diagnosis of Fanconi Anemia: Mutation Analysis by Multiplex Ligation-Dependent Probe Amplification and PCR-Based Sanger Sequencing

    Science.gov (United States)

    Gille, Johan J. P.; Floor, Karijn; Kerkhoven, Lianne; Ameziane, Najim; Joenje, Hans; de Winter, Johan P.

    2012-01-01

    Fanconi anemia (FA) is a rare inherited disease characterized by developmental defects, short stature, bone marrow failure, and a high risk of malignancies. FA is heterogeneous: 15 genetic subtypes have been distinguished so far. A clinical diagnosis of FA needs to be confirmed by testing cells for sensitivity to cross-linking agents in a chromosomal breakage test. As a second step, DNA testing can be employed to elucidate the genetic subtype of the patient and to identify the familial mutations. This knowledge allows preimplantation genetic diagnosis (PGD) and enables prenatal DNA testing in future pregnancies. Although simultaneous testing of all FA genes by next generation sequencing will be possible in the near future, this technique will not be available immediately for all laboratories. In addition, in populations with strong founder mutations, a limited test using Sanger sequencing and MLPA will be a cost-effective alternative. We describe a strategy and optimized conditions for the screening of FANCA, FANCB, FANCC, FANCE, FANCF, and FANCG and present the results obtained in a cohort of 54 patients referred to our diagnostic service since 2008. In addition, the follow up with respect to genetic counseling and carrier screening in the families is discussed. PMID:22778927

  3. The empirical power of rare variant association methods: results from sanger sequencing in 1,998 individuals.

    Directory of Open Access Journals (Sweden)

    Martin Ladouceur

    2012-02-01

    Full Text Available The role of rare genetic variation in the etiology of complex disease remains unclear. However, the development of next-generation sequencing technologies offers the experimental opportunity to address this question. Several novel statistical methodologies have been recently proposed to assess the contribution of rare variation to complex disease etiology. Nevertheless, no empirical estimates comparing their relative power are available. We therefore assessed the parameters that influence their statistical power in 1,998 individuals Sanger-sequenced at seven genes by modeling different distributions of effect, proportions of causal variants, and direction of the associations (deleterious, protective, or both in simulated continuous trait and case/control phenotypes. Our results demonstrate that the power of recently proposed statistical methods depend strongly on the underlying hypotheses concerning the relationship of phenotypes with each of these three factors. No method demonstrates consistently acceptable power despite this large sample size, and the performance of each method depends upon the underlying assumption of the relationship between rare variants and complex traits. Sensitivity analyses are therefore recommended to compare the stability of the results arising from different methods, and promising results should be replicated using the same method in an independent sample. These findings provide guidance in the analysis and interpretation of the role of rare base-pair variation in the etiology of complex traits and diseases.

  4. Identification of novel BRCA founder mutations in Middle Eastern breast cancer patients using capture and Sanger sequencing analysis.

    Science.gov (United States)

    Bu, Rong; Siraj, Abdul K; Al-Obaisi, Khadija A S; Beg, Shaham; Al Hazmi, Mohsen; Ajarim, Dahish; Tulbah, Asma; Al-Dayel, Fouad; Al-Kuraya, Khawla S

    2016-09-01

    Ethnic differences of breast cancer genomics have prompted us to investigate the spectra of BRCA1 and BRCA2 mutations in different populations. The prevalence and effect of BRCA 1 and BRCA 2 mutations in Middle Eastern population is not fully explored. To characterize the prevalence of BRCA mutations in Middle Eastern breast cancer patients, BRCA mutation screening was performed in 818 unselected breast cancer patients using Capture and/or Sanger sequencing. 19 short tandem repeat (STR) markers were used for founder mutation analysis. In our study, nine different types of deleterious mutation were identified in 28 (3.4%) cases, 25 (89.3%) cases in BRCA 1 and 3 (10.7%) cases in BRCA 2. Seven recurrent mutations identified accounted for 92.9% (26/28) of all the mutant cases. Haplotype analysis was performed to confirm c.1140 dupG and c.4136_4137delCT mutations as novel putative founder mutation, accounting for 46.4% (13/28) of all BRCA mutant cases and 1.6% (13/818) of all the breast cancer cases, respectively. Moreover, BRCA 1 mutation was significantly associated with BRCA 1 protein expression loss (p = 0.0005). Our finding revealed that a substantial number of BRCA mutations were identified in clinically high risk breast cancer from Middle East region. Identification of the mutation spectrum, prevalence and founder effect in Middle Eastern population facilitates genetic counseling, risk assessment and development of cost-effective screening strategy. © 2016 UICC.

  5. Diagnostic single gene analyses beyond Sanger. Economic high-throughput sequencing of small genes involved in congenital coagulation and platelet disorders.

    Science.gov (United States)

    Najm, Juliane; Rath, Matthias; Schröder, Winnie; Felbor, Ute

    2017-07-17

    Molecular testing of congenital coagulation and platelet disorders offers confirmation of clinical diagnoses, supports genetic counselling, and enables predictive and prenatal diagnosis. In some cases, genotype-phenotype correlations are important for predicting the clinical course of the disease and adaptation of individualized therapy. Until recently, genotyping has been mainly performed by Sanger sequencing. While next generation sequencing (NGS) enables the parallel analysis of multiple genes, the cost-value ratio of custom-made panels can be unfavorable for analyses of specific small genes. The aim of this study was to transfer genotyping of small genes involved in congenital coagulation and platelet disorders from Sanger sequencing to an NGS-based method. A LR-PCR approach for target enrichment of the entire genomic regions of the genes F7, F10, F11, F12, GATA1, MYH9, TUBB1 and WAS was combined with high-throughput sequencing on a MiSeq platform. NGS detected all variants that had previously been identified by Sanger sequencing. Our results demonstrate that this approach is an accurate and flexible tool for molecular genetic diagnostics of single small genes.

  6. SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation.

    Directory of Open Access Journals (Sweden)

    Wei Shen

    Full Text Available FASTA and FASTQ are basic and ubiquitous formats for storing nucleotide and protein sequences. Common manipulations of FASTA/Q file include converting, searching, filtering, deduplication, splitting, shuffling, and sampling. Existing tools only implement some of these manipulations, and not particularly efficiently, and some are only available for certain operating systems. Furthermore, the complicated installation process of required packages and running environments can render these programs less user friendly. This paper describes a cross-platform ultrafast comprehensive toolkit for FASTA/Q processing. SeqKit provides executable binary files for all major operating systems, including Windows, Linux, and Mac OSX, and can be directly used without any dependencies or pre-configurations. SeqKit demonstrates competitive performance in execution time and memory usage compared to similar tools. The efficiency and usability of SeqKit enable researchers to rapidly accomplish common FASTA/Q file manipulations. SeqKit is open source and available on Github at https://github.com/shenwei356/seqkit.

  7. SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation.

    Science.gov (United States)

    Shen, Wei; Le, Shuai; Li, Yan; Hu, Fuquan

    2016-01-01

    FASTA and FASTQ are basic and ubiquitous formats for storing nucleotide and protein sequences. Common manipulations of FASTA/Q file include converting, searching, filtering, deduplication, splitting, shuffling, and sampling. Existing tools only implement some of these manipulations, and not particularly efficiently, and some are only available for certain operating systems. Furthermore, the complicated installation process of required packages and running environments can render these programs less user friendly. This paper describes a cross-platform ultrafast comprehensive toolkit for FASTA/Q processing. SeqKit provides executable binary files for all major operating systems, including Windows, Linux, and Mac OSX, and can be directly used without any dependencies or pre-configurations. SeqKit demonstrates competitive performance in execution time and memory usage compared to similar tools. The efficiency and usability of SeqKit enable researchers to rapidly accomplish common FASTA/Q file manipulations. SeqKit is open source and available on Github at https://github.com/shenwei356/seqkit.

  8. Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak

    Directory of Open Access Journals (Sweden)

    Léger Patrick

    2010-11-01

    Full Text Available Abstract Background The Fagaceae family comprises about 1,000 woody species worldwide. About half belong to the Quercus family. These oaks are often a source of raw material for biomass wood and fiber. Pedunculate and sessile oaks, are among the most important deciduous forest tree species in Europe. Despite their ecological and economical importance, very few genomic resources have yet been generated for these species. Here, we describe the development of an EST catalogue that will support ecosystem genomics studies, where geneticists, ecophysiologists, molecular biologists and ecologists join their efforts for understanding, monitoring and predicting functional genetic diversity. Results We generated 145,827 sequence reads from 20 cDNA libraries using the Sanger method. Unexploitable chromatograms and quality checking lead us to eliminate 19,941 sequences. Finally a total of 125,925 ESTs were retained from 111,361 cDNA clones. Pyrosequencing was also conducted for 14 libraries, generating 1,948,579 reads, from which 370,566 sequences (19.0% were eliminated, resulting in 1,578,192 sequences. Following clustering and assembly using TGICL pipeline, 1,704,117 EST sequences collapsed into 69,154 tentative contigs and 153,517 singletons, providing 222,671 non-redundant sequences (including alternative transcripts. We also assembled the sequences using MIRA and PartiGene software and compared the three unigene sets. Gene ontology annotation was then assigned to 29,303 unigene elements. Blast search against the SWISS-PROT database revealed putative homologs for 32,810 (14.7% unigene elements, but more extensive search with Pfam, Refseq_protein, Refseq_RNA and eight gene indices revealed homology for 67.4% of them. The EST catalogue was examined for putative homologs of candidate genes involved in bud phenology, cuticle formation, phenylpropanoids biosynthesis and cell wall formation. Our results suggest a good coverage of genes involved in these

  9. Comprehensive transcriptome assembly of Chickpea (Cicer arietinum L. using sanger and next generation sequencing platforms: development and applications.

    Directory of Open Access Journals (Sweden)

    Himabindu Kudapa

    Full Text Available A comprehensive transcriptome assembly of chickpea has been developed using 134.95 million Illumina single-end reads, 7.12 million single-end FLX/454 reads and 139,214 Sanger expressed sequence tags (ESTs from >17 genotypes. This hybrid transcriptome assembly, referred to as Cicer arietinumTranscriptome Assembly version 2 (CaTA v2, available at http://data.comparative-legumes.org/transcriptomes/cicar/lista_cicar-201201, comprising 46,369 transcript assembly contigs (TACs has an N50 length of 1,726 bp and a maximum contig size of 15,644 bp. Putative functions were determined for 32,869 (70.8% of the TACs and gene ontology assignments were determined for 21,471 (46.3%. The new transcriptome assembly was compared with the previously available chickpea transcriptome assemblies as well as to the chickpea genome. Comparative analysis of CaTA v2 against transcriptomes of three legumes - Medicago, soybean and common bean, resulted in 27,771 TACs common to all three legumes indicating strong conservation of genes across legumes. CaTA v2 was also used for identification of simple sequence repeats (SSRs and intron spanning regions (ISRs for developing molecular markers. ISRs were identified by aligning TACs to the Medicago genome, and their putative mapping positions at chromosomal level were identified using transcript map of chickpea. Primer pairs were designed for 4,990 ISRs, each representing a single contig for which predicted positions are inferred and distributed across eight linkage groups. A subset of randomly selected ISRs representing all eight chickpea linkage groups were validated on five chickpea genotypes and showed 20% polymorphism with average polymorphic information content (PIC of 0.27. In summary, the hybrid transcriptome assembly developed and novel markers identified can be used for a variety of applications such as gene discovery, marker-trait association, diversity analysis etc., to advance genetics research and breeding

  10. Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species: oak.

    Science.gov (United States)

    Ueno, Saneyoshi; Le Provost, Grégoire; Léger, Valérie; Klopp, Christophe; Noirot, Céline; Frigerio, Jean-Marc; Salin, Franck; Salse, Jérôme; Abrouk, Michael; Murat, Florent; Brendel, Oliver; Derory, Jérémy; Abadie, Pierre; Léger, Patrick; Cabane, Cyril; Barré, Aurélien; de Daruvar, Antoine; Couloux, Arnaud; Wincker, Patrick; Reviron, Marie-Pierre; Kremer, Antoine; Plomion, Christophe

    2010-11-23

    The Fagaceae family comprises about 1,000 woody species worldwide. About half belong to the Quercus family. These oaks are often a source of raw material for biomass wood and fiber. Pedunculate and sessile oaks, are among the most important deciduous forest tree species in Europe. Despite their ecological and economical importance, very few genomic resources have yet been generated for these species. Here, we describe the development of an EST catalogue that will support ecosystem genomics studies, where geneticists, ecophysiologists, molecular biologists and ecologists join their efforts for understanding, monitoring and predicting functional genetic diversity. We generated 145,827 sequence reads from 20 cDNA libraries using the Sanger method. Unexploitable chromatograms and quality checking lead us to eliminate 19,941 sequences. Finally a total of 125,925 ESTs were retained from 111,361 cDNA clones. Pyrosequencing was also conducted for 14 libraries, generating 1,948,579 reads, from which 370,566 sequences (19.0%) were eliminated, resulting in 1,578,192 sequences. Following clustering and assembly using TGICL pipeline, 1,704,117 EST sequences collapsed into 69,154 tentative contigs and 153,517 singletons, providing 222,671 non-redundant sequences (including alternative transcripts). We also assembled the sequences using MIRA and PartiGene software and compared the three unigene sets. Gene ontology annotation was then assigned to 29,303 unigene elements. Blast search against the SWISS-PROT database revealed putative homologs for 32,810 (14.7%) unigene elements, but more extensive search with Pfam, Refseq_protein, Refseq_RNA and eight gene indices revealed homology for 67.4% of them. The EST catalogue was examined for putative homologs of candidate genes involved in bud phenology, cuticle formation, phenylpropanoids biosynthesis and cell wall formation. Our results suggest a good coverage of genes involved in these traits. Comparative orthologous sequences (COS

  11. 454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine KRAS mutation analysis of formalin-fixed, paraffin-embedded samples

    Directory of Open Access Journals (Sweden)

    Altimari A

    2013-08-01

    Full Text Available Annalisa Altimari,1,* Dario de Biase,2,* Giovanna De Maglio,3 Elisa Gruppioni,1 Elisa Capizzi,1 Alessio Degiovanni,1 Antonia D'Errico,1 Annalisa Pession,2 Stefano Pizzolitto,3 Michelangelo Fiorentino,1,# Giovanni Tallini2,#1Laboratory of Molecular Oncologic and Transplantation Pathology, S. Orsola-Malpighi Hospital, Bologna, 2Laboratory of Molecular Pathology, Anatomic Pathology, Bellaria Hospital, Bologna, 3Department of Pathology, S. Maria della Misericordia Hospital, Udine, Italy*These authors contributed equally to this work #These authors share senior authorshipAbstract: Detection of KRAS mutations in archival pathology samples is critical for therapeutic appropriateness of anti-EGFR monoclonal antibodies in colorectal cancer. We compared the sensitivity, specificity, and accuracy of Sanger sequencing, ARMS-Scorpion (TheraScreen® real-time polymerase chain reaction (PCR, pyrosequencing, chip array hybridization, and 454 next-generation sequencing to assess KRAS codon 12 and 13 mutations in 60 nonconsecutive selected cases of colorectal cancer. Twenty of the 60 cases were detected as wild-type KRAS by all methods with 100% specificity. Among the 40 mutated cases, 13 were discrepant with at least one method. The sensitivity was 85%, 90%, 93%, and 92%, and the accuracy was 90%, 93%, 95%, and 95% for Sanger sequencing, TheraScreen real-time PCR, pyrosequencing, and chip array hybridization, respectively. The main limitation of Sanger sequencing was its low analytical sensitivity, whereas TheraScreen real-time PCR, pyrosequencing, and chip array hybridization showed higher sensitivity but suffered from the limitations of predesigned assays. Concordance between the methods was k = 0.79 for Sanger sequencing and k > 0.85 for the other techniques. Tumor cell enrichment correlated significantly with the abundance of KRAS-mutated deoxyribonucleic acid (DNA, evaluated as ΔCt for TheraScreen real-time PCR (P = 0.03, percentage of mutation for

  12. File sharing

    NARCIS (Netherlands)

    van Eijk, N.

    2011-01-01

    File sharing’ has become generally accepted on the Internet. Users share files for downloading music, films, games, software etc. In this note, we have a closer look at the definition of file sharing, the legal and policy-based context as well as enforcement issues. The economic and cultural

  13. Phenotype-genotype correlation with Sanger sequencing identified retinol dehydrogenase 12 (RDH12) compound heterozygous variants in a Chinese family with Leber congenital amaurosis.

    Science.gov (United States)

    Li, Yun; Pan, Qing; Gu, Yang-Shun

    2017-05-01

    Leber congenital amaurosis (LCA) is a group of clinically and genetically heterogeneous retinal dystrophy. To date, 22 genes are known to be responsible for LCA, and some specific phenotypic features could provide significant prognostic information for a potential genetic etiology. This study is to identify gene variants responsible for LCA in a Chinese family using direct Sanger sequencing, with the help of phenotype-genotype correlations. A Chinese family with six members including two individuals affected with LCA was studied. All patients underwent a complete ophthalmic examination. Based on phenotype-genotype correlation, direct Sanger sequencing was performed to identify the candidate gene on all family members and normal controls. Targeted next-generation sequencing was used to exclude other known LCA genes. By Sanger sequencing, we identified two novel missense variants in the retinol dehydrogenase 12 (RDH12) gene: a c.164C>A transversion predicting a p.T55K substitution, and a c.535C>G transversion predicting a p.H179D substitution. The two affected subjects carried both RDH12 variants, while their parents and offspring carried only one of heterozygous variants, showing complete cosegregation of the variants. The compound heterozygous variants were not present in 600 normal controls. Besides, the RDH12 variants were confirmed by targeted next-generation sequencing. The RDH12 compound heterozygous variants might be the cause of the LCA family. Our study adds to the molecular spectrum of RDH12-related retinopathy and offers an effective example of the power of phenotype-genotype correlations in molecular diagnosis of LCA.

  14. Sanger sequencing as a first-line approach for molecular diagnosis of Andersen-Tawil syndrome [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Armando Totomoch-Serra

    2017-06-01

    Full Text Available In 1977, Frederick Sanger developed a new method for DNA sequencing based on the chain termination method, now known as the Sanger sequencing method (SSM.  Recently, massive parallel sequencing, better known as next-generation sequencing (NGS,  is replacing the SSM for detecting mutations in cardiovascular diseases with a genetic background. The present opinion article wants to remark that “targeted” SSM is still effective as a first-line approach for the molecular diagnosis of some specific conditions, as is the case for Andersen-Tawil syndrome (ATS. ATS is described as a rare multisystemic autosomal dominant channelopathy syndrome caused mainly by a heterozygous mutation in the KCNJ2 gene. KCJN2 has particular characteristics that make it attractive for “directed” SSM. KCNJ2 has a sequence of 17,510 base pairs (bp, and a short coding region with two exons (exon 1=166 bp and exon 2=5220 bp, half of the mutations are located in the C-terminal cytosolic domain, a mutational hotspot has been described in residue Arg218, and this gene explains the phenotype in 60% of ATS cases that fulfill all the clinical criteria of the disease. In order to increase the diagnosis of ATS we urge cardiologists to search for facial and muscular abnormalities in subjects with frequent ventricular arrhythmias (especially bigeminy and prominent U waves on the electrocardiogram.

  15. Screening for duplications, deletions and a common intronic mutation detects 35% of second mutations in patients with USH2A monoallelic mutations on Sanger sequencing.

    Science.gov (United States)

    Steele-Stallard, Heather B; Le Quesne Stabej, Polona; Lenassi, Eva; Luxon, Linda M; Claustres, Mireille; Roux, Anne-Francoise; Webster, Andrew R; Bitner-Glindzicz, Maria

    2013-08-08

    Usher Syndrome is the leading cause of inherited deaf-blindness. It is divided into three subtypes, of which the most common is Usher type 2, and the USH2A gene accounts for 75-80% of cases. Despite recent sequencing strategies, in our cohort a significant proportion of individuals with Usher type 2 have just one heterozygous disease-causing mutation in USH2A, or no convincing disease-causing mutations across nine Usher genes. The purpose of this study was to improve the molecular diagnosis in these families by screening USH2A for duplications, heterozygous deletions and a common pathogenic deep intronic variant USH2A: c.7595-2144A>G. Forty-nine Usher type 2 or atypical Usher families who had missing mutations (mono-allelic USH2A or no mutations following Sanger sequencing of nine Usher genes) were screened for duplications/deletions using the USH2A SALSA MLPA reagent kit (MRC-Holland). Identification of USH2A: c.7595-2144A>G was achieved by Sanger sequencing. Mutations were confirmed by a combination of reverse transcription PCR using RNA extracted from nasal epithelial cells or fibroblasts, and by array comparative genomic hybridisation with sequencing across the genomic breakpoints. Eight mutations were identified in 23 Usher type 2 families (35%) with one previously identified heterozygous disease-causing mutation in USH2A. These consisted of five heterozygous deletions, one duplication, and two heterozygous instances of the pathogenic variant USH2A: c.7595-2144A>G. No variants were found in the 15 Usher type 2 families with no previously identified disease-causing mutations. In 11 atypical families, none of whom had any previously identified convincing disease-causing mutations, the mutation USH2A: c.7595-2144A>G was identified in a heterozygous state in one family. All five deletions and the heterozygous duplication we report here are novel. This is the first time that a duplication in USH2A has been reported as a cause of Usher syndrome. We found that 8 of

  16. A comparison of parallel pyrosequencing and sanger clone-based sequencing and its impact on the characterization of the genetic diversity of HIV-1.

    Directory of Open Access Journals (Sweden)

    Binhua Liang

    Full Text Available BACKGROUND: Pyrosequencing technology has the potential to rapidly sequence HIV-1 viral quasispecies without requiring the traditional approach of cloning. In this study, we investigated the utility of ultra-deep pyrosequencing to characterize genetic diversity of the HIV-1 gag quasispecies and assessed the possible contribution of pyrosequencing technology in studying HIV-1 biology and evolution. METHODOLOGY/PRINCIPAL FINDINGS: HIV-1 gag gene was amplified from 96 patients using nested PCR. The PCR products were cloned and sequenced using capillary based Sanger fluorescent dideoxy termination sequencing. The same PCR products were also directly sequenced using the 454 pyrosequencing technology. The two sequencing methods were evaluated for their ability to characterize quasispecies variation, and to reveal sites under host immune pressure for their putative functional significance. A total of 14,034 variations were identified by 454 pyrosequencing versus 3,632 variations by Sanger clone-based (SCB sequencing. 11,050 of these variations were detected only by pyrosequencing. These undetected variations were located in the HIV-1 Gag region which is known to contain putative cytotoxic T lymphocyte (CTL and neutralizing antibody epitopes, and sites related to virus assembly and packaging. Analysis of the positively selected sites derived by the two sequencing methods identified several differences. All of them were located within the CTL epitope regions. CONCLUSIONS/SIGNIFICANCE: Ultra-deep pyrosequencing has proven to be a powerful tool for characterization of HIV-1 genetic diversity with enhanced sensitivity, efficiency, and accuracy. It also improved reliability of downstream evolutionary and functional analysis of HIV-1 quasispecies.

  17. Comparison of targeted next-generation sequencing and Sanger sequencing for the detection of PIK3CA mutations in breast cancer.

    Science.gov (United States)

    Arsenic, Ruza; Treue, Denise; Lehmann, Annika; Hummel, Michael; Dietel, Manfred; Denkert, Carsten; Budczies, Jan

    2015-01-01

    Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha, PIK3CA, is one of the most frequently mutated genes in breast cancer, and the mutation status of PIK3CA has clinical relevance related to response to therapy. The aim of our study was to investigate the mutation status of PIK3CA gene and to evaluate the concordance between NGS and SGS for the most important hotspot regions in exon 9 and 20, to investigate additional hotspots outside of these exons using NGS, and to correlate the PIK3CA mutation status with the clinicopathological characteristics of the cohort. In the current study, next-generation sequencing (NGS) and Sanger Sequencing (SGS) was used for the mutational analysis of PIK3CA in 186 breast carcinomas. Altogether, 64 tumors had PIK3CA mutations, 55 of these mutations occurred in exons 9 and 20. Out of these 55 mutations, 52 could also be detected by Sanger sequencing resulting in a concordance of 98.4 % between the two sequencing methods. The three mutations missed by SGS had low variant frequencies below 10 %. Additionally, 4.8 % of the tumors had mutations in exons 1, 4, 7, and 13 of PIK3CA that were not detected by SGS. PIK3CA mutation status was significantly associated with hormone receptor-positivity, HER2-negativity, tumor grade, and lymph node involvement. However, there was no statistically significant association between the PIK3CA mutation status and overall survival. Based on our study, NGS is recommended as follows: 1) for correctly assessing the mutation status of PIK3CA in breast cancer, especially for cases with low tumor content, 2) for the detection of subclonal mutations, and 3) for simultaneous mutation detection in multiple exons.

  18. Comparison of three human papillomavirus DNA detection methods: Next generation sequencing, multiplex-PCR and nested-PCR followed by Sanger based sequencing.

    Science.gov (United States)

    da Fonseca, Allex Jardim; Galvão, Renata Silva; Miranda, Angelica Espinosa; Ferreira, Luiz Carlos de Lima; Chen, Zigui

    2016-05-01

    To compare the diagnostic performance for HPV infection using three laboratorial techniques. Ninty-five cervicovaginal samples were randomly selected; each was tested for HPV DNA and genotypes using 3 methods in parallel: Multiplex-PCR, the Nested PCR followed by Sanger sequencing, and the Next_Gen Sequencing (NGS) with two assays (NGS-A1, NGS-A2). The study was approved by the Brazilian National IRB (CONEP protocol 16,800). The prevalence of HPV by the NGS assays was higher than that using the Multiplex-PCR (64.2% vs. 45.2%, respectively; P = 0.001) and the Nested-PCR (64.2% vs. 49.5%, respectively; P = 0.003). NGS also showed better performance in detecting high-risk HPV (HR-HPV) and HPV16. There was a weak interobservers agreement between the results of Multiplex-PCR and Nested-PCR in relation to NGS for the diagnosis of HPV infection, and a moderate correlation for HR-HPV detection. Both NGS assays showed a strong correlation for detection of HPVs (k = 0.86), HR-HPVs (k = 0.91), HPV16 (k = 0.92) and HPV18 (k = 0.91). NGS is more sensitive than the traditional Sanger sequencing and the Multiplex PCR to genotype HPVs, with promising ability to detect multiple infections, and may have the potential to establish an alternative method for the diagnosis and genotyping of HPV. © 2015 Wiley Periodicals, Inc.

  19. 831 Files

    Data.gov (United States)

    Social Security Administration — SSA-831 file is a collection of initial and reconsideration adjudicative level DDS disability determinations. (A few hearing level cases are also present, but the...

  20. ACONC Files

    Data.gov (United States)

    U.S. Environmental Protection Agency — ACONC files containing simulated ozone and PM2.5 fields that were used to create the model difference plots shown in the journal article. This dataset is associated...

  1. Identifiable Data Files - Denominator File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Denominator File combines Medicare beneficiary entitlement status information from administrative enrollment records with third-party payer information and GHP...

  2. Sanger Sequencing for BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del Mutation Screen on Pap Smear Cytology Samples

    Directory of Open Access Journals (Sweden)

    Sin Hang Lee

    2016-02-01

    Full Text Available Three sets of polymerase chain reaction (PCR primers were designed for heminested PCR amplification of the target DNA fragments in the human genome which include the site of BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del respectively, to prepare the templates for direct Sanger sequencing screen of these three founder mutations. With a robust PCR mixture, crude proteinase K digestate of the fixed cervicovaginal cells in the liquid-based Papanicolaou (Pap cytology specimens can be used as the sample for target DNA amplification without pre-PCR DNA extraction, purification and quantitation. The post-PCR products can be used directly as the sequencing templates without further purification or quantitation. By simplifying the frontend procedures for template preparation, the cost for screening these three founder mutations can be reduced to about US $200 per test when performed in conjunction with human papillomavirus (HPV assays now routinely ordered for cervical cancer prevention. With this projected price structure, selective patients in a high-risk population can be tested and each provided with a set of DNA sequencing electropherograms to document the absence or presence of these founder mutations in her genome to help assess inherited susceptibility to breast and ovarian cancer in this era of precision molecular personalized medicine.

  3. HUD GIS Boundary Files

    Data.gov (United States)

    Department of Housing and Urban Development — The HUD GIS Boundary Files are intended to supplement boundary files available from the U.S. Census Bureau. The files are for community planners interested in...

  4. Provider of Services File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The POS file consists of two data files, one for CLIA labs and one for 18 other provider types. The file names are CLIA and OTHER. If downloading the file, note it...

  5. FILE WINISIS

    Directory of Open Access Journals (Sweden)

    Apallidya Sitepu

    2012-07-01

    Full Text Available Winisis (CDS/ISIS for Windows menyediakan sarana yang memungkinkan pembuatan pangkalan data menjadijauh lebih mudah dibandingkan dengan versi DOS. Meskipun demikian, mengisi tabel definisi ruas (field definition table tetap memerlukan ketelitian. Cara yang paling mudah dalam menciptakan pangkalan data adalah menyalin (copy pangkalan data pihak lain dan menempatkannya dalam komputer anda.Kebutuhan untuk menyalin berkas (file dari pangkalan data lain pada umumnya muncul jika berkas tersebut terlalu rumit untuk dibuat dan diketik, misalnya berkas fonnat cetak (print format yang panjang atau label indeks (field selection table. Pangkalan data duplikat pada umumnya diperlukan untuk menyirnpan cadangan (backup cantuman pangkalan data, untuk menyediakan komputer sebagai katalog pemakai, atau untuk membuat terbitan. Artikel ini dimaksudkan untuk membantu pustakawan, dokumentalis dan arsiparis menyalin berkas atau pangkalan data Winisis. Agar dapat menyalin dengan benar, pemakai hams mengetahui direktori atau map(folder, dan berkas-berkas Winisis. Tulisan inijuga membahas prosedur memasang Winisis dalam jaringan (LAN.

  6. Decay data file based on the ENSDF file

    Energy Technology Data Exchange (ETDEWEB)

    Katakura, J. [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1997-03-01

    A decay data file with the JENDL (Japanese Evaluated Nuclear Data Library) format based on the ENSDF (Evaluated Nuclear Structure Data File) file was produced as a tentative one of special purpose files of JENDL. The problem using the ENSDF file as primary source data of the JENDL decay data file is presented. (author)

  7. PCF File Format.

    Energy Technology Data Exchange (ETDEWEB)

    Thoreson, Gregory G [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-08-01

    PCF files are binary files designed to contain gamma spectra and neutron count rates from radiation sensors. It is the native format for the GAmma Detector Response and Analysis Software (GADRAS) package [1]. It can contain multiple spectra and information about each spectrum such as energy calibration. This document outlines the format of the file that would allow one to write a computer program to parse and write such files.

  8. Standard interface file handbook

    Energy Technology Data Exchange (ETDEWEB)

    Shapiro, A.; Huria, H.C. (Cincinnati Univ., OH (United States))

    1992-10-01

    This handbook documents many of the standard interface file formats that have been adopted by the US Department of Energy to facilitate communications between and portability of, various large reactor physics and radiation transport software packages. The emphasis is on those files needed for use of the VENTURE/PC diffusion-depletion code system. File structures, contents and some practical advice on use of the various files are provided.

  9. Patient Assessment File (PAF)

    Data.gov (United States)

    Department of Veterans Affairs — The Patient Assessment File (PAF) database compiles the results of the Patient Assessment Instrument (PAI) questionnaire filled out for intermediate care Veterans...

  10. FHEO Filed Cases

    Data.gov (United States)

    Department of Housing and Urban Development — The dataset is a list of all the Title VIII fair housing cases filed by FHEO from 1/1/2007 - 12/31/2012 including the case number, case name, filing date, state and...

  11. Download this PDF file

    African Journals Online (AJOL)

    Fr. Ikenga

    funding is available, e-filing and e-payment systems may not be efficient because the underlying ... 6C S Amaefule, et. al, The Efficacy of E-Taxation as a Strategic Tool for National Economic Development. ... 11 Y Wang, The Adoption of Electronic Tax Filing Systems: An Empirical Study, (2002) Government Information.

  12. Value-Based File Retention: File Attributes as File Value and Information Waste Indicators

    NARCIS (Netherlands)

    Wijnhoven, Alphonsus B.J.M.; Amrit, Chintan Amrit; Dietz, Pim

    2014-01-01

    Several file retention policy methods propose that a file retention policy should be based on file value. Though such a retention policy might increase the value of accessible files, the method to arrive at such a policy is underresearched. This article discusses how one can arrive at a method for

  13. Hospital Service Area File

    Data.gov (United States)

    U.S. Department of Health & Human Services — This file is derived from the calendar year inpatient claims data. The records contain number of discharges, length of stay, and total charges summarized by provider...

  14. USEEIO Satellite Files

    Data.gov (United States)

    U.S. Environmental Protection Agency — These files contain the environmental data as particular emissions or resources associated with a BEA sectors that are used in the USEEIO model. They are organized...

  15. Patient Treatment File (PTF)

    Data.gov (United States)

    Department of Veterans Affairs — This database is part of the National Medical Information System (NMIS). The Patient Treatment File (PTF) contains a record for each inpatient care episode provided...

  16. Case File: The Spazzoids

    Science.gov (United States)

    ... Washing Experiment John Snow, the First Epidemiologist Microbe Multiplication Magic Scientific Poster Session Poisoned Picnic The Energy ... Help: How do I view different file formats (PDF, DOC, PPT, MPEG) on this site? Adobe PDF ...

  17. Closed Claim Query File

    Data.gov (United States)

    Social Security Administration — This file is used to hold information about disability claims that have been closed and have been selected for sampling.Sampling is the process whereby OQR reviews...

  18. Provider of Services File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The POS file contains data on characteristics of hospitals and other types of healthcare facilities, including the name and address of the facility and the type of...

  19. National Downloadable File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Physician Compare downloadable file is organized at the individual provider level; each line is unique at the provider/enrollment/organization/address level....

  20. INTERNET: PENGIRIMAN FILE

    Directory of Open Access Journals (Sweden)

    Zainul Bakri

    2012-10-01

    Full Text Available Salah satu alasan pengguna komputer untuk berhubungan dengan Internet adalah mendapat kesempatan untuk menyalin ('download' informasi yang tersimpan dari server jaringan komputer lain (misalnya menyalin program aplikasi komputer, data mentah, dan sebagainya. File Transfer  Protocol (FfP adalah cara di Internet untuk mengirim file dari satu tempat ke komputer pengguna. Untuk keperluan ini dapat menggunakan program FTP khusus atau dengan menggunakan Web browser.

  1. File System Virtual Appliances

    Science.gov (United States)

    2010-05-01

    version. The result is over 300 configurations. 14 · File system virtual appliances Type Description Memory Pressure Some RedHat Enterprise Linux 3 kernels...blocking. This results in minutes-long de- lays in dirty data writeback under low memory situations. RedHat acknowledged the semantic mismatch but did not...ten. This caused a file system to mistakenly assume that all pages were written, resulting in data corruption. Stack Size RedHat Enterprise Linux

  2. Generic file format

    Science.gov (United States)

    Felgate, Nick

    2002-11-01

    The Generic File Format (GFF) is a file format developed within the UK ASW community for the interchange and storage of underwater sonar data. Originally developed for the interchange of time-series data between analysis systems, it has been extended to provide for storage of processed acoustic data (e.g., power and DEMON spectrum, lofargram grey-scale), nonacoustic data (e.g., own-ship dynamics, sensor configuration) and event data (e.g., tracker output, sonar intercepts). The format employs the chunk concept, as used in the WAV and AIFF file formats, to provide extendability (including local variants) while providing a measure of backward compatability. However, the basic concept has been adapted to allow for the mixing in the one file of multiple channels of different sample-rates and data-types through the inclusion of a data frame concept and multiple data blocks. Chunk cross-referencing has been employed to ensure data consistency. A provision is made in the header of the file to store details of the sensor and processing for the data (e.g., the number of hydrophones, beam direction, FFT size) so that an analysis system does not need to know about the sensor or other system from which the data originated.

  3. 76 FR 39757 - Filing Procedures

    Science.gov (United States)

    2011-07-06

    ... System, a web-based software system which will receive and store electronic transmissions of filing... applicable hardware and software requirements for electronic filing. (2) To file or submit a document in... written consent thereto to the Secretary or the presiding administrative law judge. L. Copyright and Other...

  4. Product identification file

    Energy Technology Data Exchange (ETDEWEB)

    Gray, C.E. (ed.)

    1978-06-01

    This product identification file was compiled as an aid to the industrial hygienist who may encounter the products listed in surveys for and studies of occupational health hazards. It is pointed out that the chemical composition of a product may vary from year to year and some components may be added or deleted without an indication on the label. Some of the data in this file may not be complete depending on the analysis requested. For example, a solvent may be the only component for which the product was analyzed. The file is arranged by listing the chemical manufacturer, followed by the trade name. In cases where no manufacturer is known, the trade name appears in alphabetical order. The log number and the chemist who analyzed the product are listed for reference.

  5. NASA Thesaurus Data File

    Science.gov (United States)

    2012-01-01

    The NASA Thesaurus contains the authorized NASA subject terms used to index and retrieve materials in the NASA Aeronautics and Space Database (NA&SD) and NASA Technical Reports Server (NTRS). The scope of this controlled vocabulary includes not only aerospace engineering, but all supporting areas of engineering and physics, the natural space sciences (astronomy, astrophysics, planetary science), Earth sciences, and the biological sciences. The NASA Thesaurus Data File contains all valid terms and hierarchical relationships, USE references, and related terms in machine-readable form. The Data File is available in the following formats: RDF/SKOS, RDF/OWL, ZThes-1.0, and CSV/TXT.

  6. Accessing files in an internet - The Jade file system

    Science.gov (United States)

    Rao, Herman C.; Peterson, Larry L.

    1993-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  7. Accessing files in an Internet: The Jade file system

    Science.gov (United States)

    Peterson, Larry L.; Rao, Herman C.

    1991-01-01

    Jade is a new distribution file system that provides a uniform way to name and access files in an internet environment. It makes two important contributions. First, Jade is a logical system that integrates a heterogeneous collection of existing file systems, where heterogeneous means that the underlying file systems support different file access protocols. Jade is designed under the restriction that the underlying file system may not be modified. Second, rather than providing a global name space, Jade permits each user to define a private name space. These private name spaces support two novel features: they allow multiple file systems to be mounted under one directory, and they allow one logical name space to mount other logical name spaces. A prototype of the Jade File System was implemented on Sun Workstations running Unix. It consists of interfaces to the Unix file system, the Sun Network File System, the Andrew File System, and FTP. This paper motivates Jade's design, highlights several aspects of its implementation, and illustrates applications that can take advantage of its features.

  8. Download this PDF file

    African Journals Online (AJOL)

    2004-04-22

    Apr 22, 2004 ... could be the upgrading of the Operating System along with its related files, drivers etc. Any program that attempts to do such on its own should trigger a warning, alert the user and be prevented from making such modification. The same applied to Boot Sector writers and the modifications of interrupt vectors.

  9. Download this PDF file

    African Journals Online (AJOL)

    approaches. The authors herein evaluate their experience with this technique at the hospital of Cham- bery, France. Patients and Methods: The files of all patients subjected to laparoscopic adrenalec- tomy at the department of General and Thoracic Surgery between 1996 and 2000 were reviewed. A partial lateral decubitus ...

  10. Download this PDF file

    African Journals Online (AJOL)

    2004-04-19

    Apr 19, 2004 ... near the Norman, Oklahoma, municipal landfill, 1995: U.S. Geological. Survey Open File Report 95-825, 125pp. Odufisan, B., 1991. Electrical resistivity survey for groundwater in Benin. Owena Basin Development Authority (BORBDA). Proposed perma- nent site–Akure, Ondo State. Unpublished B.Sc.

  11. Download this PDF file

    African Journals Online (AJOL)

    User

    five years of age. The covariance components were estimated by “restricted maximum likelihood” using the WOMBAT software (Meyer, 2006). In all of the analyses, a pedigree file containing the identification of the animal, sire, and dam was used, with a total of 26 924 animals in the relationship matrix. Spearman correlation.

  12. Download this PDF file

    African Journals Online (AJOL)

    personal

    status on the severity of periodontal disease. Materials and Methods: A one year retrospective study of 298 patients who had been treated at the periodontics clinic of the University College Hospital,. Ibadan was conducted. Case file records were reviewed and information on patients' bio-data, occupation and periodontal ...

  13. 20 CFR 217.16 - Filing date.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Filing date. 217.16 Section 217.16 Employees... LUMP SUM Filing An Application § 217.16 Filing date. An application filed in a manner and form acceptable to the Board is officially filed with the Board on the earliest of the following dates: (a) On the...

  14. PFS: a distributed and customizable file system

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.

    1996-01-01

    In this paper we present our ongoing work on the Pegasus File System (PFS), a distributed and customizable file system that can be used for off-line file system experiments and on-line file system storage. PFS is best described as an object-oriented component library from which either a true file

  15. Huygens File Service and Storage Architecture

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.; Stabell-Kulo, Tage; Stabell-Kulo, Tage

    1993-01-01

    The Huygens file server is a high-performance file server which is able to deliver multi-media data in a timely manner while also providing clients with ordinary “Unix” like file I/O. The file server integrates client machines, file servers and tertiary storage servers in the same storage

  16. Huygens file service and storage architecture

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.; Stabell-Kulo, Tage; Stabell-Kulo, Tage

    1993-01-01

    The Huygens file server is a high-performance file server which is able to deliver multi-media data in a timely manner while also providing clients with ordinary “Unix” like file I/O. The file server integrates client machines, file servers and tertiary storage servers in the same storage

  17. MERRA-2: File Specification

    Science.gov (United States)

    Bosilovich, M. G.; Lucchesi, R.; Suarez, M.

    2015-01-01

    The second Modern-Era Retrospective analysis for Research and Applications (MERRA-2) is a NASA atmospheric reanalysis that begins in 1980. It replaces the original MERRA reanalysis (Rienecker et al., 2011) using an upgraded version of the Goddard Earth Observing System Model, Version 5 (GEOS-5) data assimilation system. The file collections for MERRA-2 are described in detail in this document, including some important changes from those of the MERRA dataset (Lucchesi, 2012).

  18. 78 FR 23251 - Notice of Agreements Filed

    Science.gov (United States)

    2013-04-18

    ... Service Agreement and Hanjin Shipping. Filing Party: Paul M. Keane, Esq.; Cichanowicz, Callan, Keane... Company Limited. ] Filing Party: Paul M. Keane, Esq.; Cichanowicz, Callan, Keane, Vengrow & Textor, LLP...

  19. Earnings Public-Use File, 2006

    Data.gov (United States)

    Social Security Administration — Social Security Administration released Earnings Public-Use File (EPUF) for 2006. File contains earnings information for individuals drawn from a systematic random...

  20. Converting Inhouse Subject Card Files to Electronic Keyword Files.

    Science.gov (United States)

    Culmer, Carita M.

    The library at Phoenix College developed the Controversial Issues Files (CIF), a "home made" card file containing references pertinent to specific ongoing assignments. Although the CIF had proven itself to be an excellent resource tool for beginning researchers, it was cumbersome to maintain in the card format, and was limited to very…

  1. The DNA Files

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-06-09

    The DNA Files is a radio documentary which disseminates genetics information over public radio. The documentaries explore subjects which include the following: How genetics affects society. How human life began and how it evolved. Could new prenatal genetic tests hold the key to disease prevention later in life? Would a national genetic data base sacrifice individual privacy? and Should genes that may lead to the cure for cancer be privately owned? This report serves as a project update for the second quarter of 1998. It includes the spring/summer 1998 newsletter, the winter 1998 newsletter, the program clock, and the latest flyer.

  2. 78 FR 17394 - Filing via the Internet; Electronic Tariff Filings; Revisions to Electric Quarterly Report Filing...

    Science.gov (United States)

    2013-03-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Filing via the Internet; Electronic Tariff Filings; Revisions to Electric... will be available. Off-site participants interested in attending via teleconference or viewing the...

  3. 10 CFR 603.1020 - File documents.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false File documents. 603.1020 Section 603.1020 Energy... Reporting Information About the Award § 603.1020 File documents. The award file should include an analysis..., the file must document the analysis required (see § 603.560) to set the fixed level of Federal support...

  4. 25 CFR 2.13 - Filing documents.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Filing documents. 2.13 Section 2.13 Indians BUREAU OF... § 2.13 Filing documents. (a) An appeal document is properly filed with an official of the Bureau of... for receipt of mail addressed to the official; the document is considered filed by mail on the date...

  5. 77 FR 20810 - Combined Notice of Filings

    Science.gov (United States)

    2012-04-06

    ...: Big Sandy-EQT Capacity Release Waiver Request to be effective N/A. Filed Date: 3/30/12. Accession... and Sales Report of Rockies Express Pipeline LLC. Filed Date: 3/30/12. Accession Number: 20120330-5140.... Description: QEP 37657-15 Amendment to Negotiated Rate Agreement filing to be effective 4/1/2012. Filed Date...

  6. 48 CFR 1404.802 - Contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract files. 1404.802 Section 1404.802 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL ADMINISTRATIVE MATTERS Contract Files 1404.802 Contract files. In addition to the requirements in FAR 4.802, files shall...

  7. Detecting Malicious Code by Binary File Checking

    Directory of Open Access Journals (Sweden)

    Marius POPA

    2014-01-01

    Full Text Available The object, library and executable code is stored in binary files. Functionality of a binary file is altered when its content or program source code is changed, causing undesired effects. A direct content change is possible when the intruder knows the structural information of the binary file. The paper describes the structural properties of the binary object files, how the content can be controlled by a possible intruder and what the ways to identify malicious code in such kind of files. Because the object files are inputs in linking processes, early detection of the malicious content is crucial to avoid infection of the binary executable files.

  8. Penyimpanan File Pribadi User Berbasis Web Cloud

    OpenAIRE

    Aulia, T. Mirza

    2017-01-01

    142406161 Pada penulisan tugas akhir ini, penulis membuat aplikasi penyimpanan file pribadi user berbasis web cloud. Aplikasi ini merupakan sebuah website penyimpanan file-file user. Seiring berkembanganya teknologi dibutuhkan juga web penyimpanan file,atau disebut juga dengan cloud,salah satunya seperti google drive,penulis membuat program ini karena terinspirasi melihat kejadian Mahasiswa USU bunuh diri akibat file skirpsinya hilang karena laptopnya dicuri. Berdasarkan hal tersebut penul...

  9. Performance optimization for managing massive numbers of small files in distributed file systems

    OpenAIRE

    Fu, Songling; He, Ligang; Huang, Chenlin; Liao, Xiangke; Li, Kenli

    2014-01-01

    The processing of massive numbers of small files is a challenge in the design of distributed file systems. Currently, the combined-block-storage approach is prevalent. However, the approach employs the traditional file systems such as ExtFS and may cause inefficiency when accessing small files randomly located in the disk. This paper focuses on optimizing the performance of data servers in accessing massive numbers of small files. We present a Flat Lightweight File System (iFlatLFS) to manage...

  10. PKA spectrum file

    Energy Technology Data Exchange (ETDEWEB)

    Kawai, M. [Toshiba Corp., Kawasaki, Kanagawa (Japan). Nuclear Engineering Lab.

    1997-03-01

    In the Japanese Nuclear Data Committee, the PKA/KERMA file containing PKA spectra, KERMA factors and DPA cross sections in the energy range between 10{sup -5} eV and 50 MeV is being prepared from the evaluated nuclear data. The processing code ESPERANT was developed to calculate quantities of PKA, KERMA and DPA from evaluated nuclear data for medium and heavy elements by using the effective single particle emission approximation (ESPEA). For light elements, the PKA spectra are evaluated by the SCINFUL/DDX and EXIFON codes, simultaneously with other neutron cross sections. The DPA cross sections due to charged particle emitted from light elements are evaluated for high neutron energy above 20 MeV. (author)

  11. Denominator File - Limited Data Set

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Denominator File combines Medicare beneficiary entitlement status information from administrative enrollment records with third-party payer information and GHP...

  12. FRS Geospatial Return File Format

    Science.gov (United States)

    The Geospatial Return File Format describes format that needs to be used to submit latitude and longitude coordinates for use in Envirofacts mapping applications. These coordinates are stored in the Geospatail Reference Tables.

  13. Physician Compare National Downloadable File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Physician Compare National Downloadable File is organized at the individual eligible professional level; each line is unique at the professional/enrollment...

  14. Value Modifier Public Use File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Center for Medicare (CM) has created a standard analytical file intended to promote transparency. For each Value Modifier performance year, CM will publish a...

  15. XML Files: MedlinePlus

    Science.gov (United States)

    ... this page: https://medlineplus.gov/xml.html MedlinePlus XML Files To use the sharing features on this page, please enable JavaScript. MedlinePlus produces XML data sets that you are welcome to download ...

  16. Cytoscape file of chemical networks

    Data.gov (United States)

    U.S. Environmental Protection Agency — The maximum connectivity scores of pairwise chemical conditions summarized from Cmap results in a file with Cytoscape format (http://www.cytoscape.org/). The figures...

  17. Search the SEC website for the latest EDGAR filings

    Data.gov (United States)

    Securities and Exchange Commission — This listing contains the most recent filings for the current official filing date (including filings made after the 5:30pm deadline on the previous filing day)....

  18. Usage analysis of user files in UNIX

    Science.gov (United States)

    Devarakonda, Murthy V.; Iyer, Ravishankar K.

    1987-01-01

    Presented is a user-oriented analysis of short term file usage in a 4.2 BSD UNIX environment. The key aspect of this analysis is a characterization of users and files, which is a departure from the traditional approach of analyzing file references. Two characterization measures are employed: accesses-per-byte (combining fraction of a file referenced and number of references) and file size. This new approach is shown to distinguish differences in files as well as users, which cam be used in efficient file system design, and in creating realistic test workloads for simulations. A multi-stage gamma distribution is shown to closely model the file usage measures. Even though overall file sharing is small, some files belonging to a bulletin board system are accessed by many users, simultaneously and otherwise. Over 50% of users referenced files owned by other users, and over 80% of all files were involved in such references. Based on the differences in files and users, suggestions to improve the system performance were also made.

  19. Cut-and-Paste file-systems: integrating simulators and file systems

    NARCIS (Netherlands)

    Bosch, H.G.P.; Mullender, Sape J.

    1995-01-01

    We have implemented an integrated and configurable file system called the Pegasus filesystem (PFS) and a trace-driven file-system simulator called Patsy. Patsy is used for off-line analysis of file-systemalgorithms, PFS is used for on-line file-systemdata storage. Algorithms are first analyzed in

  20. 29 CFR 102.98 - Petition for advisory opinion; who may file; where to file.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 2 2010-07-01 2010-07-01 false Petition for advisory opinion; who may file; where to file... REGULATIONS, SERIES 8 Declaratory Orders and Advisory Opinions Regarding Board Jurisdiction § 102.98 Petition for advisory opinion; who may file; where to file. Whenever an agency or court of any State or...

  1. Did accelerated filing requirements and SOX Section 404 affect the timeliness of 10-K filings?

    NARCIS (Netherlands)

    J. Impink (Joost); M. Lubberink (Martien); B. van Praag (Bart); D. Veenman (David)

    2012-01-01

    textabstractThis paper examines the effect of Sarbanes-Oxley provisions on 10-K filing delays. We find that tightened filing deadlines for accelerated and large accelerated filers are not associated with changes in the incidence of late filing. While Section 404 compliance does not affect filing

  2. Did accelerated filing requirements and SOX section 404 affect the timeliness of 10-K filings?

    NARCIS (Netherlands)

    Impink, J.; Lubberink, M.; van Praag, B.; Veenman, D.

    2010-01-01

    This paper examines the effect of Sarbanes-Oxley provisions on 10-K filing delays. We find that tightened filing deadlines for accelerated and large accelerated filers are not associated with changes in the incidence of late filing; that Section 404 compliance does not affect filing timeliness for

  3. Did Accelerated Filing Requirements and Sox Section 404 Affect the Timeliness of 10-K Filings?

    NARCIS (Netherlands)

    Impink, J.; Lubberink, M.; van Praag, B.; Veenman, D.

    2012-01-01

    This paper examines the effect of Sarbanes-Oxley provisions on 10-K filing delays. We find that tightened filing deadlines for accelerated and large accelerated filers are not associated with changes in the incidence of late filing. While Section 404 compliance does not affect filing timeliness for

  4. 37 CFR 1.54 - Parts of application to be filed together; filing receipt.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Parts of application to be filed together; filing receipt. 1.54 Section 1.54 Patents, Trademarks, and Copyrights UNITED STATES... Processing Provisions The Application § 1.54 Parts of application to be filed together; filing receipt. (a...

  5. 33 CFR 148.246 - When is a document considered filed and where should I file it?

    Science.gov (United States)

    2010-07-01

    ... filed and where should I file it? 148.246 Section 148.246 Navigation and Navigable Waters COAST GUARD... Formal Hearings § 148.246 When is a document considered filed and where should I file it? (a) If a document to be filed is submitted by mail, it is considered filed on the date it is postmarked. If a...

  6. Physician Fee Schedule Carrier Specific Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Centers for Medicare and Medicaid Services (CMS) has condensed all 56 Physician Fee Schedule (PFS) carrier specific pricing files into one zip file. It is...

  7. source files for manuscript in tex format

    Data.gov (United States)

    U.S. Environmental Protection Agency — Source tex files used to create the manuscript including original figure files and raw data used in tables and inline text. This dataset is associated with the...

  8. 76 FR 30333 - Combined Notice of Filings

    Science.gov (United States)

    2011-05-25

    ... Report filings: Docket Numbers: RP11-2051-000. Applicants: Black Marlin Pipeline Company. Description: Report of Black Marlin Pipeline Company. Filed Date: 04/28/2011. Accession Number: 20110428-5219. Comment...

  9. Synchronizing Defense Transportation System Reference Files

    National Research Council Canada - National Science Library

    Frohman, Harold

    1998-01-01

    .... Many DTS AISs frequently use outdated reference files. As a result, electronic transactions and documents containing reference file information have data errors that cause those AISs to reject the transactions or report erroneous information...

  10. Fermi FT2 Spacecraft Pointing Files

    Data.gov (United States)

    National Aeronautics and Space Administration — This utility permits you to download the most current version of the spacecraft (FT2) file predicting the LAT's pointing for a given mission week. The FT2 file is a...

  11. 12 CFR 303.221 - Filing procedures.

    Science.gov (United States)

    2010-01-01

    ... PROCEDURES Section 19 of the FDI Act (Consent to Service of Persons Convicted of Certain Criminal Offenses) § 303.221 Filing procedures. (a) Where to file. An application under section 19 of the FDI Act shall be...

  12. Physician-Supplier Procedure Summary Master File

    Data.gov (United States)

    U.S. Department of Health & Human Services — This file is a 100 percent summary of all Part B Carrier and DMERC Claims processed through the Common Working File and stored in the National Claims History...

  13. 77 FR 74655 - Combined Notice of Filings

    Science.gov (United States)

    2012-12-17

    ... Numbers: RP12-1066-001. Applicants: Big Sandy Pipeline, LLC. Description: Big Sandy Pipeline, LLC submits tariff filing per 154.203: RP12-1066 Compliance Filing to be effective 12/1/2012. Filed Date: 12/10/12.../1/2012. Filed Date: 12/10/12. Accession Number: 20121210-5172. Comments Due: 5 p.m. ET 12/24/12...

  14. 77 FR 47834 - Combined Notice of Filings

    Science.gov (United States)

    2012-08-10

    ..., LLC. Description: Big Sandy Fuel Filing effective 9-1-2012 to be effective 9/1/2012. Filed Date: 7/31... Negotiated Rate to be effective 8/ 1/2012. Filed Date: 7/31/12. Accession Number: 20120731-5120. Comments Due...: Compliance with CP12-5-000 to be effective 9/1/2012. Filed Date: 7/31/12. Accession Number: 20120731-5129...

  15. 11 CFR 9006.2 - Filing dates.

    Science.gov (United States)

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Filing dates. 9006.2 Section 9006.2 Federal Elections FEDERAL ELECTION COMMISSION PRESIDENTIAL ELECTION CAMPAIGN FUND: GENERAL ELECTION FINANCING REPORTS AND RECORDKEEPING § 9006.2 Filing dates. The reports required to be filed under 11 CFR 9006.1...

  16. 12 CFR 19.100 - Filing documents.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Filing documents. 19.100 Section 19.100 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY RULES OF PRACTICE AND PROCEDURE Procedural Rules for OCC Adjudications § 19.100 Filing documents. All materials required to be filed with or...

  17. 47 CFR 76.1701 - Political file.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Political file. 76.1701 Section 76.1701... CABLE TELEVISION SERVICE Documents to be Maintained for Inspection § 76.1701 Political file. (a) Every... (political file) of all requests for cablecast time made by or on behalf of a candidate for public office...

  18. 47 CFR 73.1943 - Political file.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Political file. 73.1943 Section 73.1943... Rules Applicable to All Broadcast Stations § 73.1943 Political file. (a) Every licensee shall keep and permit public inspection of a complete and orderly record (political file) of all requests for broadcast...

  19. 76 FR 45788 - Combined Notice of Filings

    Science.gov (United States)

    2011-08-01

    ...: Big Sandy LINK Implementation to be effective 9/1/2011. Filed Date: 07/14/2011. Accession Number...: ETNG Modifications for Big Sandy LINK Implementation to be effective 9/1/2011. Filed Date: 07/14/2011... Big Sandy LINK Implementation to be effective 9/1/2011. Filed Date: 07/14/2011. Accession Number...

  20. FileMaker Pro 9

    CERN Document Server

    Coffey, Geoff

    2007-01-01

    FileMaker Pro 9: The Missing Manual is the clear, thorough and accessible guide to the latest version of this popular desktop database program. FileMaker Pro lets you do almost anything with the information you give it. You can print corporate reports, plan your retirement, or run a small country -- if you know what you're doing. This book helps non-technical folks like you get in, get your database built, and get the results you need. Pronto.The new edition gives novices and experienced users the scoop on versions 8.5 and 9. It offers complete coverage of timesaving new features such as the Q

  1. Capability File Names over HTTP

    OpenAIRE

    Owais, Fahad

    2010-01-01

    File access over the Internet adds a whole new dimension to the continuously evolving World Wide Web. Considering its significance in the big picture, there exists a need to provide a completely secure and  exible way to share and access content existing on a file host web server somewhere over the Internet. A number of access control mechanisms attempt to do this with moderate success with most of them lacking the ability to allow sharing of information to unauthenticated users and adequate ...

  2. The Crystallographic Information File (CIF

    Directory of Open Access Journals (Sweden)

    I D Brown

    2006-11-01

    Full Text Available The Crystallographic Information File (CIF, owned by the International Union of Crystallography, is a file structure based on tag-value ASCII pairs with tags defined in machine-readable dictionaries. The crystallographic community publishes and archives large quantities of numeric information generated by crystal structure determinations, and CIF's acceptance was assured by its adoption as the submission format for Acta Crystallographica and by the obvious needs of the community. CIF's strength lies in its dictionaries, which define most of the concepts of crystallography; its weakness is the difficulty of writing software that exploits its full potential.

  3. Protecting your files on the DFS file system

    CERN Multimedia

    Computer Security Team

    2011-01-01

    The Windows Distributed File System (DFS) hosts user directories for all NICE users plus many more data.    Files can be accessed from anywhere, via a dedicated web portal (http://cern.ch/dfs). Due to the ease of access to DFS with in CERN it is of utmost importance to properly protect access to sensitive data. As the use of DFS access control mechanisms is not obvious to all users, passwords, certificates or sensitive files might get exposed. At least this happened in past to the Andrews File System (AFS) - the Linux equivalent to DFS) - and led to bad publicity due to a journalist accessing supposedly "private" AFS folders (SonntagsZeitung 2009/11/08). This problem does not only affect the individual user but also has a bad impact on CERN's reputation when it comes to IT security. Therefore, all departments and LHC experiments agreed recently to apply more stringent protections to all DFS user folders. The goal of this data protection policy is to assist users in pro...

  4. Protecting your files on the AFS file system

    CERN Multimedia

    2011-01-01

    The Andrew File System is a world-wide distributed file system linking hundreds of universities and organizations, including CERN. Files can be accessed from anywhere, via dedicated AFS client programs or via web interfaces that export the file contents on the web. Due to the ease of access to AFS it is of utmost importance to properly protect access to sensitive data in AFS. As the use of AFS access control mechanisms is not obvious to all users, passwords, private SSH keys or certificates have been exposed in the past. In one specific instance, this also led to bad publicity due to a journalist accessing supposedly "private" AFS folders (SonntagsZeitung 2009/11/08). This problem does not only affect the individual user but also has a bad impact on CERN's reputation when it comes to IT security. Therefore, all departments and LHC experiments agreed in April 2010 to apply more stringent folder protections to all AFS user folders. The goal of this data protection policy is to assist users in...

  5. Adolescent Fertility: National File [Machine-Readable Data File].

    Science.gov (United States)

    Moore, Kristin A.; And Others

    This computer file contains recent cross sectional data on adolescent fertility in the United States for 1960, 1965, 1970, 1975 and 1980-85. The following variables are included: (1) births; (2) birth rates; (3) abortions; (4) non-marital childbearing; (5) infant mortality; and (6) low birth weight. Data for both teenagers and women aged 20-24 are…

  6. A Metadata-Rich File System

    Energy Technology Data Exchange (ETDEWEB)

    Ames, S; Gokhale, M B; Maltzahn, C

    2009-01-07

    Despite continual improvements in the performance and reliability of large scale file systems, the management of file system metadata has changed little in the past decade. The mismatch between the size and complexity of large scale data stores and their ability to organize and query their metadata has led to a de facto standard in which raw data is stored in traditional file systems, while related, application-specific metadata is stored in relational databases. This separation of data and metadata requires considerable effort to maintain consistency and can result in complex, slow, and inflexible system operation. To address these problems, we have developed the Quasar File System (QFS), a metadata-rich file system in which files, metadata, and file relationships are all first class objects. In contrast to hierarchical file systems and relational databases, QFS defines a graph data model composed of files and their relationships. QFS includes Quasar, an XPATH-extended query language for searching the file system. Results from our QFS prototype show the effectiveness of this approach. Compared to the defacto standard, the QFS prototype shows superior ingest performance and comparable query performance on user metadata-intensive operations and superior performance on normal file metadata operations.

  7. Deceit: A flexible distributed file system

    Science.gov (United States)

    Siegel, Alex; Birman, Kenneth; Marzullo, Keith

    1989-01-01

    Deceit, a distributed file system (DFS) being developed at Cornell, focuses on flexible file semantics in relation to efficiency, scalability, and reliability. Deceit servers are interchangeable and collectively provide the illusion of a single, large server machine to any clients of the Deceit service. Non-volatile replicas of each file are stored on a subset of the file servers. The user is able to set parameters on a file to achieve different levels of availability, performance, and one-copy serializability. Deceit also supports a file version control mechanism. In contrast with many recent DFS efforts, Deceit can behave like a plain Sun Network File System (NFS) server and can be used by any NFS client without modifying any client software. The current Deceit prototype uses the ISIS Distributed Programming Environment for all communication and process group management, an approach that reduces system complexity and increases system robustness.

  8. Registering Researchers in Authority Files

    Science.gov (United States)

    Smith-Yoshimura, Karen; Altman, Micah; Conlon, Michael; Cristán, Ana Lupe; Dawson, Laura; Dunham, Joanne; Hickey, Thom; Hook, Daniel; Horstmann, Wolfram; MacEwan, Andrew; Schreur, Philip; Smart, Laura; Wacker, Melanie; Woutersen, Saskia

    2014-01-01

    Written by OCLC Research Program Officer Karen Smith-Yoshimura and the 13 members of the Registering Researchers in Authority Files Task Group comprised of specialists from the US, UK, and the Netherlands, this report summarizes their research into approaches to providing authoritative researcher identifiers. Registering researchers in some type…

  9. Analyzing data files in SWAN

    CERN Document Server

    Gajam, Niharika

    2016-01-01

    Traditionally analyzing data happens via batch-processing and interactive work on the terminal. The project aims to provide another way of analyzing data files: A cloud-based approach. It aims to make it a productive and interactive environment through the combination of FCC and SWAN software.

  10. SPEM: a parser for EMBL style flat file database entries.

    Science.gov (United States)

    Pocock, M R; Hubbard, T; Birney, E

    1998-01-01

    We present a set of Perl modules for the flexible and robust parsing and editing of EMBL/SWISS-PROT databases. The Web page at http://www.sanger.ac. uk/Software/PerlModule/ provides information about downloading the SPEM and PrEMBL modules, and provides links to documentation and example code.

  11. Flat Files - JSNP | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ... Data file File name: jsnp_flat_files File URL: ftp://ftp.biosciencedbc.jp/archiv...his Database Database Description Download License Update History of This Database Site Policy | Contact Us Flat Files - JSNP | LSDB Archive ...

  12. 76 FR 61684 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-10-05

    ... Limited submits tariff filing per 35.1: Eighth Amendments to Agreements for Sale and Purchase of Capacity... submits tariff filing per 35.17(b): Amendment to Filing of Interconnection Agreement with Lowell...): Service Agreement No. 174, Amendment Type Filing to be effective 12/31/9998. Filed Date: 09/26/2011...

  13. High-throughput next-generation sequencing to genotype six classical HLA loci from 96 donors in a single MiSeq run.

    Science.gov (United States)

    Ehrenberg, P K; Geretz, A; Sindhu, R K; Vayntrub, T; Fernández Viña, M A; Apps, R; Michael, N L; Thomas, R

    2017-11-01

    Next generation sequencing (NGS) methods have been established as an efficient approach for HLA typing because unlike traditional Sanger sequencing, they provide unambiguous results at a reasonable cost. We previously developed a multi-locus index method to genotype four HLA loci (A, B, C, and DRB1) on the Illumina MiSeq platform. We have now expanded this method to include two additional loci, HLA-DPB1 and DQB1. Contiguous full-length amplicons from 5'UTR through 3'UTR regions were generated using one long-range PCR reaction per locus for each of the six loci from 96 individuals of different ethnicities. The six amplicons from each donor were pooled, enzymatically fragmented and given a donor-specific index. This approach enabled sequencing of 576 loci from 96 individuals in a single MiSeq run. Donor-specific sequence reads were demultiplexed, and allele calls were generated from FASTQ files using commercially available software. Comparison to HLA genotypes generated from Sanger sequence-based typing (SBT) identified no discordances among any of the alleles analyzed in this study. Importantly, this method was able to resolve 22 DPB1 and 20 DQB1 alleles that were ambiguous with the SBT method. Furthermore, a novel allele in each of these two loci was identified, with the DQB1*05:01:24 allele having a frequency of greater than five percent. This method was subsequently validated against a blinded panel of 22 samples from the 17th International HLA and Immunogenetics Workshop. The flexibility of the method is further highlighted by successful genotyping of eight loci comprising all classical HLA loci for a subset of the samples. We now present a high-throughput, high-resolution, scalable NGS HLA typing method to accurately and efficiently genotype all classical HLA class I and II loci. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  14. Converting Spreadsheet Files into Data Base Files Using AppleWorks V2.0 and the Apple IIGS Computer.

    Science.gov (United States)

    Schlenker, Richard M.

    This guide was developed as a "how to" training device for converting spreadsheet files into database files using AppleWorks version 2.0 on the Apple IIGS computer with two disk drives. Step-by-step instructions are provided for loading spreadsheet files, transferring spreadsheet files to the monitor, printing spreadsheet files, and…

  15. Storage of sparse files using parallel log-structured file system

    Energy Technology Data Exchange (ETDEWEB)

    Bent, John M.; Faibish, Sorin; Grider, Gary; Torres, Aaron

    2017-11-07

    A sparse file is stored without holes by storing a data portion of the sparse file using a parallel log-structured file system; and generating an index entry for the data portion, the index entry comprising a logical offset, physical offset and length of the data portion. The holes can be restored to the sparse file upon a reading of the sparse file. The data portion can be stored at a logical end of the sparse file. Additional storage efficiency can optionally be achieved by (i) detecting a write pattern for a plurality of the data portions and generating a single patterned index entry for the plurality of the patterned data portions; and/or (ii) storing the patterned index entries for a plurality of the sparse files in a single directory, wherein each entry in the single directory comprises an identifier of a corresponding sparse file.

  16. File Type Identification of File Fragments using Longest Common Subsequence (LCS)

    Science.gov (United States)

    Rahmat, R. F.; Nicholas, F.; Purnamawati, S.; Sitompul, O. S.

    2017-01-01

    Computer forensic analyst is a person in charge of investigation and evidence tracking. In certain cases, the file needed to be presented as digital evidence was deleted. It is difficult to reconstruct the file, because it often lost its header and cannot be identified while being restored. Therefore, a method is required for identifying the file type of file fragments. In this research, we propose Longest Common Subsequences that consists of three steps, namely training, testing and validation, to identify the file type from file fragments. From all testing results we can conlude that our proposed method works well and achieves 92.91% of accuracy to identify the file type of file fragment for three data types.

  17. Assessment of Insert Sizes and Adapter Content in Fastq Data from NexteraXT Libraries

    Directory of Open Access Journals (Sweden)

    Frances Susan Turner

    2014-01-01

    Full Text Available The Illumina NexteraXT transposon protocol is a cost effective way to generate paired end libraries. However the resulting insert size is highly sensitive to the concentration of DNA used, and the variation of insert sizes is often large. One consequence of this is some fragments may have an insert shorter than the length of a single read, particularly where the library is designed to produce overlapping paired end reads in order to produce longer continuous sequences. Such small insert sizes mean fewer longer reads, and also result in the presence of adapter at the end of the read. Here is presented a protocol to use publicly available tools to identify read pairs with small insert sizes and so likely to contain adapter, to check the sequence of the adapter, and remove adapter sequence from the reads. This protocol does not require a reference genome or prior knowledge of the sequence to be trimmed. Whilst the presence of fragments with small insert sizes may be a particular problem for NexteraXT libraries, the principle can be applied to any Illumina dataset in which the presence of such small inserts is suspected.

  18. Evaluated Nuclear Structure Data File

    Science.gov (United States)

    Tuli, Jagdish K.

    2004-10-01

    The Evaluated Nuclear Structure Data File (ENSDF) is a leading resource for the experimental nuclear data. It is maintained and distributed by the National Nuclear Data Center, Brookhaven National Laboratory. The file is mainly contributed to by an international network of evaluators under the auspice of the International Atomic Energy Agency. The ENSDF is updated, generally by mass number, i.e., evaluating together all isobars for a given mass number. If, however, experimental activity in an isobaric chain is limited to a particular nuclide then only that nuclide is updated. The evaluations are published in the journal Nuclear Data Sheets, a publication of Elsevier. This presentation will briefly review this and other databases and dissemination services of the US and international network, and reflect on how the network resources can help scientists in both the basic as well as the applied fields.

  19. 76 FR 63294 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-10-12

    ... Filings 1 Take notice that the Commission received the following electric rate filings: Docket Numbers.... 123 Merchant Substation Maintenance Agreement to be effective 10/1/2011. Filed Date: 10/03/2011...

  20. City locations for all places in the TIGER files

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — City locations for all places in the TIGER files; this file was extracted from dbf files posted on the internet by the Bureau of the Census. This is basically a...

  1. 76 FR 43679 - Filing via the Internet; Notice of Additional File Formats for efiling

    Science.gov (United States)

    2011-07-21

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Filing via the Internet; Notice of Additional File Formats for efiling Take notice that the Commission has added to its list of acceptable file formats the four...

  2. Forensic analysis of video file formats

    National Research Council Canada - National Science Library

    Gloe, Thomas; Fischer, André; Kirchner, Matthias

    2014-01-01

    .... In combination, such characteristics can help to authenticate digital video files in forensic settings by distinguishing between original and post-processed videos, verifying the purported source...

  3. WFC3/UVIS: Bias Reference Files Analysis

    Science.gov (United States)

    McKay, M.; Baggett, S.

    2017-11-01

    We created and delivered new superbias reference files for the WFC3/UVIS channel and analyzed the data for temporal variation in the residual two-dimensional bias structure. Previously, the pipeline only provided superbias reference files for 2009 to 2010 in which the 2010 file was used for all the following years. We updated the 2009 and 2010 reference files and created new reference files for 2009 to 2016. The files were constructed from stacks of standard bias frames taken from 2009-2016. Statistics of the reference files were calculated and plotted as a function of time to display any significant changes in the data. The difference between the previous and current superbias files is 0.02 electrons for 2009 and 0.04 electrons for 2010. The analysis shows that the overall residual bias level gradually increased by 0.16 electrons from 2009 - 2016. In this report, we briefly discuss the process we used to generate the bias reference files, the process to check for significant changes in the biases and results of the analysis.

  4. Shirky and Sanger, or the costs of crowdsourcing

    Directory of Open Access Journals (Sweden)

    Mathieu O'Neil

    2010-03-01

    Full Text Available Online knowledge production sites do not rely on isolated experts but on collaborative processes, on the wisdom of the group or “crowd”. Some authors have argued that it is possible to combine traditional or credentialled expertise with collective production; others believe that traditional expertise's focus on correctness has been superseded by the affordances of digital networking, such as re-use and verifiability. This paper examines the costs of two kinds of “crowdsourced” encyclopedic projects: Citizendium, based on the work of credentialled and identified experts, faces a recruitment deficit; in contrast Wikipedia has proved wildly popular, but anti-credentialism and anonymity result in uncertainty, irresponsibility, the development of cliques and the growing importance of pseudo-legal competencies for conflict resolution. Finally the paper reflects on the wider social implications of focusing on what experts are rather than on what they are for.

  5. selecting suitable drainage pattern to minimize flooding in sangere ...

    African Journals Online (AJOL)

    Mr Takana

    in the contour interpolation using Simple kriging method, with a precession of 0.001m, a vector layer for existing drainages was created. The spatial data obtained from the ground survey method was used in the production of the vector layer map. The coordinates were used in plotting the drainage geometry and delineation ...

  6. DATA Act File C Award Financial - Social Security

    Data.gov (United States)

    Social Security Administration — The DATA Act Information Model Schema Reporting Submission Specification File C. File C includes the agency award information from the financial accounting system at...

  7. Experiences on File Systems: Which is the best file system for you?

    CERN Document Server

    Blomer, J

    2015-01-01

    The distributed file system landscape is scattered. Besides a plethora of research file systems, there is also a large number of production grade file systems with various strengths and weaknesses. The file system, as an abstraction of permanent storage, is appealing because it provides application portability and integration with legacy and third-party applications, including UNIX utilities. On the other hand, the general and simple file system interface makes it notoriously difficult for a distributed file system to perform well under a variety of different workloads. This contribution provides a taxonomy of commonly used distributed file systems and points out areas of research and development that are particularly important for high-energy physics.

  8. 77 FR 13604 - Notice of Agreements Filed

    Science.gov (United States)

    2012-03-07

    ....; Hyundai Merchant Marine Co., Ltd.; Industrial Maritime Carriers, L.L.C.; Nordana Line A/ S; and Rickmers...., Ltd; Hyundai Merchant Marine Co., Ltd; and Nippon Yusen Kaisha. Filing Party: David F. Smith, Esquire.... Pte, Ltd.; Hyundai Merchant Marine Co., Ltd.; and Mitsui O.S.K. Lines, Ltd. Filing Party: Wayne R...

  9. 9 CFR 392.5 - Filing procedures.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Filing procedures. 392.5 Section 392.5 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOOD SAFETY AND... interested person may file a petition with FSIS. For purposes of this part, an “interested person” is any...

  10. New details emerge from the Einstein files

    CERN Multimedia

    Overbye, D

    2002-01-01

    For many years the FBI spied on Einstein. New details of this surveilance are emerging in "The Einstein File: J. Edgar Hoover's Secret War Against the World's Most Famous Scientist," by Fred Jerome, who sued the government with the help of the Public Citizen Litigation Group to obtain a less censored version of the file (1 page).

  11. 76 FR 11473 - Notice of Agreements Filed

    Science.gov (United States)

    2011-03-02

    ... Space Charter Agreement. Parties: A.P. Moeller-Maersk A/S and Mediterranean Shipping Company S.A. Filing...; American President Lines, Ltd.; Maersk Line Limited; and Zim Integrated Shipping Services, Ltd. Filing...) Company/China Shipping Container Lines, Co. Ltd.; COSCO Container Lines Company, Ltd.; Evergreen Line...

  12. 12 CFR 1412.7 - Filing instructions.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Filing instructions. 1412.7 Section 1412.7 Banks and Banking FARM CREDIT SYSTEM INSURANCE CORPORATION GOLDEN PARACHUTE AND INDEMNIFICATION PAYMENTS § 1412.7 Filing instructions. Requests to make excess nondiscriminatory severance plan payments and...

  13. 78 FR 61995 - Combined Notice of Filings

    Science.gov (United States)

    2013-10-10

    ...: Virginia Power Energy Marketing Inc. Description: Petition of Virginia Power Energy Marketing, Inc. for Temporary Waiver of Capacity Release Regulations and Policies, and Request for Expedited Action. Filed Date..., Petition for Temporary Waiver of Capacity Release Rules and Request for Shortened Comment Period. Filed...

  14. Aspects on Transfer of Aided - Design Files

    Science.gov (United States)

    Goanta, A. M.; Anghelache, D. G.

    2016-08-01

    At this stage of development of hardware and software, each company that makes design software packages has a certain type of file created and customized in time to distinguish that company from its competitors. Thus today are widely known the DWG files belonging AutoCAD, IPT / IAM belonging to Inventor, PAR / ASM of Solid Edge's, PRT from the NX and so on. Behind every type of file there is a mathematical model which is common to more types of files. A specific aspect of the computer -aided design is that all softwares are working with both individual parts and assemblies, but their approach is different in that some use the same type of file both for each part and for the whole (PRT ), while others use different types of files (IPT / IAM, PAR / ASM, etc.). Another aspect of the computer -aided design is to transfer files between different companies which use different software packages or even the same software package but in different versions. Each of these situations generates distinct issues. Thus, to solve the partial reading by a project different from the native one, transfer files of STEP and IGES type are used

  15. 78 FR 49493 - Combined Notice of Filings

    Science.gov (United States)

    2013-08-14

    ... following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket Numbers: RP13-1119-000. Applicants: East Tennessee Natural Gas, LLC. Description: FOSA Clean-up to be...: Central New York Oil And Gas, L.L.C. Description: Central New York Oil And Gas Company LLC--Order No. 776...

  16. 76 FR 68437 - Combined Notice of Filings

    Science.gov (United States)

    2011-11-04

    ... following Natural Gas Pipeline Rate and Refund Report filings: Filings Instituting Proceedings Docket.... Applicants: Colorado Interstate Gas Company. Description: Operational Purchases and Sales Report for the 12.... Applicants: Central New York Oil And Gas, LLC. Description: Central New York Oil And Gas, LLC submits tariff...

  17. 75 FR 67357 - Combined Notice of Filings

    Science.gov (United States)

    2010-11-02

    ... has received the following Natural Gas Pipeline Rate and Refund Report filings: Docket Numbers: RP11...: RP11-1433-000. Applicants: Central New York Oil and Gas, LLC. Description: Central New York Oil and Gas...: Northern Natural Gas Company. Description: Northern Natural Gas Company submits tariff filing per 154.204...

  18. 37 CFR 401.16 - Electronic filing.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Electronic filing. 401.16... OF COMMERCE RIGHTS TO INVENTIONS MADE BY NONPROFIT ORGANIZATIONS AND SMALL BUSINESS FIRMS UNDER GOVERNMENT GRANTS, CONTRACTS, AND COOPERATIVE AGREEMENTS § 401.16 Electronic filing. Unless otherwise...

  19. X-Files opjat v stroju

    Index Scriptorium Estoniae

    2008-01-01

    USA sarjale "The X-Files" põhinev teine järjefilm "Salatoimikud: Ma tahan uskuda" ("The X-Files: I Want to Believe") : režissöör Chris Carter : peaosades David Duchovny, Gillian Anderson : Ameerika Ühendriigid - Kanada 2008

  20. 77 FR 59921 - Combined Notice of Filings

    Science.gov (United States)

    2012-10-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings Take notice that the Commission has received the... Jurisdictional Blanket Certificate of Public Convenience and necessity, pursuant to Section 284.224. Filed Date...

  1. A Survey on Distributed File System Technology

    Science.gov (United States)

    Blomer, J.

    2015-05-01

    Distributed file systems provide a fundamental abstraction to location-transparent, permanent storage. They allow distributed processes to co-operate on hierarchically organized data beyond the life-time of each individual process. The great power of the file system interface lies in the fact that applications do not need to be modified in order to use distributed storage. On the other hand, the general and simple file system interface makes it notoriously difficult for a distributed file system to perform well under a variety of different workloads. This has lead to today's landscape with a number of popular distributed file systems, each tailored to a specific use case. Early distributed file systems merely execute file system calls on a remote server, which limits scalability and resilience to failures. Such limitations have been greatly reduced by modern techniques such as distributed hash tables, content-addressable storage, distributed consensus algorithms, or erasure codes. In the light of upcoming scientific data volumes at the exabyte scale, two trends are emerging. First, the previously monolithic design of distributed file systems is decomposed into services that independently provide a hierarchical namespace, data access, and distributed coordination. Secondly, the segregation of storage and computing resources yields to a storage architecture in which every compute node also participates in providing persistent storage.

  2. Investigation of At-Risk Patent Filings

    Science.gov (United States)

    Livne, O.

    2003-01-01

    The author presents an investigation of patent-application filings made without external financial support, or "at-risk", based on inventions disclosed to the University of California from fiscal years 1991 to 2000. The success of the at-risk patent applications filed on these invention disclosures is examined from the perspective of…

  3. 76 FR 1421 - Combined Notice of Filings

    Science.gov (United States)

    2011-01-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings December 30, 2010. Take notice that the.... Description: National Fuel Gas Supply Corporation submits tariff filing per 154.204: 2011 January IG Rate to...

  4. 76 FR 33748 - Combined Notice of Filings

    Science.gov (United States)

    2011-06-09

    ... Date: 05/24/2011. Accession Number: 20110524-5121. Comment Date: 5 p.m. Eastern Time on Monday, June 06, 2011. Docket Numbers: RP11-2125-000. Applicants: Big Sandy Pipeline, LLC. Description: Big Sandy Pipeline, LLC submits tariff filing per 154.204: Big Sandy Contract Assignment Filing to be effective 6/1...

  5. Athos: Efficient Authentication of Outsourced File Systems

    DEFF Research Database (Denmark)

    Triandopoulos, Nikolaos; Goodrich, Michael T.; Papamanthou, Charalampos

    2008-01-01

    outsourced storage. Using light-weight cryptographic primitives and efficient data-structuring techniques, we design authentication schemes that allow a client to efficiently verify that the file system is fully consistent with the exact history of updates and queries requested by the client. In Athos, file...

  6. 76 FR 41331 - Application Filing Requirements

    Science.gov (United States)

    2011-07-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision Application Filing Requirements AGENCY: Office of Thrift Supervision (OTS.... Title of Proposal: Application Filing Requirements. OMB Number: 1550-0056. Form Number: N/A. Description...

  7. 76 FR 26802 - Application Filing Requirements

    Science.gov (United States)

    2011-05-09

    ... Office of Thrift Supervision Application Filing Requirements AGENCY: Office of Thrift Supervision (OTS... Treasury will submit the proposed information collection requirement described below to the Office of... collection. Title of Proposal: Application Filing Requirements. OMB Number: 1550-0056. Form Number: N/A...

  8. 78 FR 72877 - Combined Notice of Filings

    Science.gov (United States)

    2013-12-04

    ... Cash-Out Refund Report of Southern Star Central Gas Pipeline, Inc. Filed Date: 11/22/13. Accession...: RP14-200-000. Applicants: Southern Star Central Gas Pipeline, Inc. Description: Annual Operational Flow Order Report of Southern Star Central Gas Pipeline, Inc. Filed Date: 11/21/13. Accession Number...

  9. Environmental Baseline File: National Transportation

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-05-22

    This Environmental Baseline File summarizes and consolidates information related to the national-level transportation of commercial spent nuclear fuel. Topics address include: shipmnents of commercial spent nuclear fuel based on mostly truck and mostly rail shipping scenarios; transportation routing for commercial spent nuclear fuel sites and DOE sites; radionuclide inventories for various shipping container capacities; transportation routing; populations along transportation routes; urbanized area population densities; the impacts of historical, reasonably foreseeable, and general transportation; state-level food transfer factors; Federal Guidance Report No. 11 and 12 radionuclide dose conversion factors; and national average atmospheric conditions.

  10. JENDL decay data file 2015

    OpenAIRE

    片倉 純一; 湊 太志

    2016-01-01

    JENDL Decay Data File 2015 (JENDL/DDF-2015) has been produced. The decay data of nuclides with mass numbers from 1 to 260 are included. The nuclides with unknown gamma- and/or beta-emission are also included in order to keep decay chains. The data of 1,284 fission product nuclides with mass from 66 to 172 remain unchanged from JENDL/FPD-2011 except several corrections which had been claimed by users, and those of the newly added 1,953 nuclides are taken from ENSDF. Finally, the decay data of ...

  11. 7 CFR 29.92 - Record of filing time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Record of filing time. 29.92 Section 29.92 Agriculture... INSPECTION Regulations Appeal § 29.92 Record of filing time. When an appeal is filed, the date and time of filing shall be recorded by the officer receiving it. ...

  12. 76 FR 30331 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2011-05-25

    ... Refund Report filings: Docket Numbers: RP11-2102-000. Applicants: Big Sandy Pipeline, LLC. Description: Big Sandy Pipeline, LLC submits tariff filing per 154.204: Big Sandy Negotiated Rate Agreement Filing to be effective 6/ 1/2011. Filed Date: 05/11/2011. Accession Number: 20110511-5167. Comment Date: 5 p...

  13. 78 FR 20910 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-04-08

    ... Complex. Description: Compliance filing to be effective 8/26/2010. Filed Date: 3/28/13. Accession Number... following electric corporate filings: Docket Numbers: EC13-86-000. Applicants: ITC Midwest LLC. Description: Application for Approval of Acquisition of Transmission Assets of ITC Midwest LLC. Filed Date: 3/27/13...

  14. The Design of a High-Performance File Server

    NARCIS (Netherlands)

    van Renesse, R.; Tanenbaum, A.S.; Wilschut, A.N.

    The Bullet server is a file server that outperforms traditional file servers by more than a factor of three. It achieves high throughput and low delay by a software design radically different from that of file servers currently in use. Whereas files are normally stored as a sequence of disk blocks,

  15. JENDL FP decay data file 2000

    Energy Technology Data Exchange (ETDEWEB)

    Katakura, Jun-ichi [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Yoshida, Tadashi [Musashi Inst. of Tech., Tokyo (Japan); Oyamatsu, Kazuhiro [Aichi Shukutoku Univ., Nagakute, Aichi (Japan); Tachibana, Takahiro [Waseda Univ., Tokyo (Japan)

    2001-07-01

    A decay data file of fission product (FP) nuclides has been developed for the use in nuclear technology field as one of special purpose files of JENDL (Japanese Evaluated Nuclear Data Library) in the format of ENDF/B and it is called JENDL FP Decay Data File 2000. The file includes the decay data for 1229 fission product nuclides: 142 stable and 1087 unstable nuclides. The data included for a nuclide are decay modes, their Q values and branching ratios, average decay energy values of beta-rays, gamma-rays and alpha-particles and their spectral data. The primary source of the decay data is ENSDF (Evaluated Nuclear Structure Data File), which is the internationally recognized data file of nuclear structure properties. The data in ENSDF, however, cover only measured ones. The data of the short-lived nuclides needed for the application fields such as decay heat prediction are often incomplete or not measured because of their short half-lives. For such nuclides a theoretical model calculation is applied to derive the needed data such as average decay energies and spectral data. The data in JENDL FP Decay Data File 2000 have been tested by summation calculation comparing its results with measured data of decay heat values and aggregate fission product spectra of various fissioning nuclei. The comparison showed good agreement between the calculated results and the measured values. (author)

  16. Interoperability format translation and transformation between IFC architectural design file and simulation file formats

    Science.gov (United States)

    Chao, Tian-Jy; Kim, Younghun

    2015-01-06

    Automatically translating a building architecture file format (Industry Foundation Class) to a simulation file, in one aspect, may extract data and metadata used by a target simulation tool from a building architecture file. Interoperability data objects may be created and the extracted data is stored in the interoperability data objects. A model translation procedure may be prepared to identify a mapping from a Model View Definition to a translation and transformation function. The extracted data may be transformed using the data stored in the interoperability data objects, an input Model View Definition template, and the translation and transformation function to convert the extracted data to correct geometric values needed for a target simulation file format used by the target simulation tool. The simulation file in the target simulation file format may be generated.

  17. Online file sharing innovations in media consumption

    CERN Document Server

    Andersson Schwarz, Jonas

    2013-01-01

    It is apparent that file sharing on the Internet has become an emerging norm of media consumption-especially among young people. This book provides a critical perspective on this phenomenon, exploring issues related to file sharing, downloading, peer-to-peer networks, ""piracy,"" and (not least) policy issues regarding these practices. Andersson Schwartz critically engages with the justificatory discourses of the actual file-sharers, taking Sweden as a geographic focus. By focusing on the example of Sweden-home to both The Pirate Bay and Spotify-he provides a unique insight into a mentality th

  18. Analyzing Log Files using Data-Mining

    Directory of Open Access Journals (Sweden)

    Marius Mihut

    2008-01-01

    Full Text Available Information systems (i.e. servers, applications and communication devices create a large amount of monitoring data that are saved as log files. For analyzing them, a data-mining approach is helpful. This article presents the steps which are necessary for creating an ‘analyzing instrument’, based on an open source software called Waikato Environment for Knowledge Analysis (Weka [1]. For exemplification, a system log file created by a Windows-based operating system, is used as input file.

  19. 21 CFR 1301.14 - Filing of application; acceptance for filing; defective applications.

    Science.gov (United States)

    2010-04-01

    ... OF JUSTICE REGISTRATION OF MANUFACTURERS, DISTRIBUTORS, AND DISPENSERS OF CONTROLLED SUBSTANCES... will not generally be accepted for filing. In the case of minor defects as to completeness, the...

  20. 77 FR 16838 - Notice of Agreements Filed

    Science.gov (United States)

    2012-03-22

    ... Sailing Agreement. Parties: Dole Ocean Cargo Express, Inc. and Great White Fleet (US) Ltd. Filing Party... name of Great White Fleet (US) Ltd. to Great White Fleet Liner Services Ltd. Agreement No.: 011961-011...

  1. 76 FR 23573 - Combined Notice of Filings

    Science.gov (United States)

    2011-04-27

    ..., May 02, 2011. Docket Numbers: RP11-2014-000. Applicants: Black Marlin Pipeline Company. Description: Black Marlin Pipeline Company submits tariff filing per 154.204: Gas Quality Waiver to be effective 5/1...

  2. 78 FR 27217 - Combined Notice of Filings

    Science.gov (United States)

    2013-05-09

    .... Docket Numbers: RP13-847-000. Applicants: Black Marlin Pipeline Company. Description: Annual Cash-Out Report of Black Marlin Pipeline Company. Filed Date: 4/30/13. Accession Number: 20130430-5187. Comments...

  3. 77 FR 27039 - Combined Notice of Filings

    Science.gov (United States)

    2012-05-08

    ...: 5 p.m. ET 5/8/12. Docket Numbers: RP12-651-000. Applicants: Black Marlin Pipeline Company. Description: Black Marlin Pipeline Company submits Annual Imbalance Cash-out Report. Filed Date: 4/26/12...

  4. 78 FR 26779 - Notice of Agreements Filed

    Science.gov (United States)

    2013-05-08

    ...). Agreement No.: 012206. Title: Industria Armamento Meridionale S.P.A./K-Line Space Charter Agreement. Parties: Industria Armamento Meridionale S.P.A. (``Inarme'') and Kawasaki Kisen Kaisha, Ltd. Filing Party: John P...

  5. 12 CFR 303.121 - Filing procedures.

    Science.gov (United States)

    2010-01-01

    ... writing to the appropriate FDIC office. (b) Contents of filing. A complete letter notice or letter... part 362 of this chapter; (iii) A copy of the bank's business plan regarding the conduct of the...

  6. 78 FR 10172 - Notice of Agreements Filed

    Science.gov (United States)

    2013-02-13

    ... Harbor & Terminal District and Federal Marine Terminals, Inc. (FMT). Filing Party: C. Jonathan Benner; Thompson Coburn LLC; 1909 K Street NW. Suite 600, Washington, DC 20006. Synopsis: The agreement permits FMT...

  7. NCPC Central Files Information System (CFIS)

    Data.gov (United States)

    National Capital Planning Commission — This dataset contains records from NCPC's Central Files Information System (CFIS), which is a comprehensive database of projects submitted to NCPC for design review...

  8. FDIC Summary of Deposits (SOD) Download File

    Data.gov (United States)

    Federal Deposit Insurance Corporation — The FDIC's Summary of Deposits (SOD) download file contains deposit data for branches and offices of all FDIC-insured institutions. The Federal Deposit Insurance...

  9. 76 FR 18219 - Notice of Agreement Filed

    Science.gov (United States)

    2011-04-01

    ... Line Joint Service Agreement and A.P. Moller- Maersk A/S. Filing Party: Paul M. Keane, Esq.; Cichanowicz, Callan, Keane, Vengrow & Textor, LLP; 61 Broadway, Suite 3000, New York, NY 10006-2802 Synopsis...

  10. 78 FR 63474 - Notice of Agreements Filed

    Science.gov (United States)

    2013-10-24

    ...: Evergreen Line Joint Service Agreement and Hanjin Shipping Co. Ltd. Filing Party: Paul M. Keane, Esq.; Cichanowicz, Callan, Keane, Vengrow and Textor, LLP; 61 Broadway, Suite 3000; New York, NY 10006. Synopsis...

  11. 75 FR 78245 - Notice of Agreements Filed

    Science.gov (United States)

    2010-12-15

    ...: Evergreen Line Joint Service Agreement and A.P. Moller- Maersk A/S. Filing Party: Paul M. Keane, Esq.; Cichanowicz, Callan, Keane, Vengrow & Textor, LLP; 61 Broadway, Suite 3000, New York, NY 10006- 2802. Synopsis...

  12. IntentToFileWebService

    Data.gov (United States)

    Department of Veterans Affairs — A veteran can initiate an Intent To File (ITF) a claim for VBA benefits by initiating a VBA claim via multiple web enabled front ends or by submitting a paper claim...

  13. Medicare Geographic Variation - Public Use File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare Geographic Variation Public Use File provides the ability to view demographic, utilization and quality indicators at the state level (including...

  14. Health Insurance Marketplace Public Use Files

    Data.gov (United States)

    U.S. Department of Health & Human Services — A set of seven (7) public use files containing information on health insurance issuers participating in the Health Insurance Marketplace and certified qualified...

  15. 77 FR 31346 - Combined Notice of Filings

    Science.gov (United States)

    2012-05-25

    ... Numbers: RP12-732-000. Applicants: Golden Triangle Storage, Inc. Description: Revisions to Request for.... Description: Negotiated Rate Service Agreement--Rice to be effective 5/16/2012. Filed Date: 5/15/12. Accession...

  16. National Drug File - Reference Terminology API

    Data.gov (United States)

    U.S. Department of Health & Human Services — The National Drug File - Reference Terminology (NDF-RT) is produced by the U.S. Department of Veterans Affairs, Veterans Health Administration (VHA). NDF-RT is an...

  17. Identifiable Data Files - Health Outcomes Survey (HOS)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare Health Outcomes Survey (HOS) identifiable data files are comprised of the entire national sample for a given 2-year cohort (including both respondents...

  18. 75 FR 42445 - Notice of Agreement Filed

    Science.gov (United States)

    2010-07-21

    ... No.: 012105. Title: SCM Lines Transportes/CCNI Agreement. Parties: Compania Chilena de Navegacion Interoceanica S.A. and SCM Lines Transportes Maritimos Sociedade Unipessoal, LDA. Filing Party: John P. Vayda...

  19. 75 FR 12748 - Notice of Agreements Filed

    Science.gov (United States)

    2010-03-17

    ... ( http://www.fmc.gov ) or by contacting the Office of Agreements at (202)-523-5793 or [email protected]fmc...; Yangming Marine Transport Corporation; and Zim Integrated Shipping Services, Ltd. Filing Party: Joshua P...

  20. 78 FR 36781 - Notice of Agreements Filed

    Science.gov (United States)

    2013-06-19

    ... ( www.fmc.gov ) or by contacting the Office of Agreements at (202)-523-5793 or [email protected]fmc.gov... River Stevedores, Inc. and South Jersey Port Corporation. Filing Party: Eugene Mattioni; Suite 200-399...

  1. Identifiable Data Files - Medicare Provider Analysis and ...

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Medicare Provider Analysis and Review (MEDPAR) File contains data from claims for services provided to beneficiaries admitted to Medicare certified inpatient...

  2. 75 FR 23749 - Combined Notice of Filings

    Science.gov (United States)

    2010-05-04

    ...-Mississippi River Transmission Corporation submits an amended negotiated rate agreement between MRT and LER... amended negotiated rate agreement between MRT and CES. Filed Date: 04/26/2010. Accession Number: 20100426...

  3. Radiology Teaching Files on the Internet

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Eun Chung; Kim, Eun Kyung [Dept. of Oral and Maxillofacial Radiology, College of Dentistry, DonKook University, Seoul (Korea, Republic of)

    1996-08-15

    There is increasing attention about radiology teaching files on the Internet in the field of diagnostic radiology. The purpose of this study was to aid in the creation of new radiology teaching file by analysing the present radiology teaching file sites on the Internet with many aspects and evaluating images on those sites, using Macintosh II ci compute r, 28.8kbps TelePort Fax/Modem, Netscape Navigator 2.0 software. The results were as follow : 1. Analysis of radiology teaching file sites (1) Country distribution was the highest in USA (57.5%). (2) Average number of cases was 186 cases and radiology teaching file sites with search engine were 9 sites (22.5%). (3) At the method of case arrangement, anatomic area type and diagnosis type were found at the 10 sites (25%) each, question and answer type was found at the 9 sites (22.5%). (4) Radiology teaching file sites with oro-maxillofacial disorder were 9 sites (22.5%). (5) At the image format, GIF format was found at the 14 sites (35%), and JPEG format found at the 14 sites (35%). (6) Created year was the highest in 1995 (43.7%). (7) Continuing case upload was found at the 35 sites (87.5%). 2. Evaluation of images on the radiology teaching files (1) Average file size of GIF format (71 Kbyte) was greater than that of JPEG format (24 Kbyte). (P<0.001) (2) Image quality of GIF format was better than that of JPEG format. (P<0.001)

  4. 77 FR 61587 - Combined Notice of Filings

    Science.gov (United States)

    2012-10-10

    ....P. Description: 10/01/12 FERC Order 587-V NAESB 2.0 to be effective 12/1/2012. Filed Date: 10/1/12...: 20121001-5448. Comments Due: 5 p.m. ET 10/15/12. Docket Numbers: RP13-106-000. Applicants: Young Gas... Numbers: RP13-1-000. Applicants: KO Transmission Company. Description: Order No. 587-V Compliance Filing...

  5. 76 FR 30336 - Combined Notice of Filings

    Science.gov (United States)

    2011-05-25

    ... Rates 2011-05-04 to be effective 5/5/ 2011. Filed Date: 05/04/2011. Accession Number: 20110504-5159. Comment Date: 5 p.m. Eastern Time on Monday, May 16, 2011. Docket Numbers: RP11-2092-000. Applicants: Big... effective 4/1/ 2011. Filed Date: 04/04/2011. Accession Number: 20110404-5138. Comment Date: 5 p.m. Eastern...

  6. Divorce Filing as Role Behavior: Effect of No-Fault Law on Divorce Filing Patterns

    Science.gov (United States)

    Gunter, B. G.; Johnson, Doyle P.

    1978-01-01

    Study contrasts male-female behavior in official initiation and completion of divorce proceedings before and after implementation of "no-fault" divorce law. Passage of no-fault law was followed by reversal in male and female filing and completion patterns, with males filing and completing more frequently under "no-fault"…

  7. 77 FR 12830 - Pershing County Water Conservation District; Notice of Intent To File License Application, Filing...

    Science.gov (United States)

    2012-03-02

    ... Energy Regulatory Commission Pershing County Water Conservation District; Notice of Intent To File....: 14327-000. c. Date Filed: November 22, 2011. d. Submitted by: Pershing County Water Conservation... Applicant Contact: Bennie Hodges, Pershing County Water Conservation District, P.O. Box 218, Lovelock, NV...

  8. 76 FR 70651 - Fee for Filing a Patent Application Other Than by the Electronic Filing System

    Science.gov (United States)

    2011-11-15

    ... the Electronic Filing System AGENCY: United States Patent and Trademark Office, Commerce. ACTION... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE... for a design, plant, or provisional application, that is not filed by electronic means as prescribed...

  9. Large-scale File System Design and Architecture

    Directory of Open Access Journals (Sweden)

    V. Dynda

    2002-01-01

    Full Text Available This paper deals with design issues of a global file system, aiming to provide transparent data availability, security against loss and disclosure, and support for mobile and disconnected clients.First, the paper surveys general challenges and requirements for large-scale file systems, and then the design of particular elementary parts of the proposed file system is presented. This includes the design of the raw system architecture, the design of dynamic file replication with appropriate data consistency, file location and data security.Our proposed system is called Gaston, and will be referred further in the text under this name or its abbreviation GFS (Gaston File System.

  10. Contents of GPS Data Files

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, John P. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Carver, Matthew Robert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Norman, Benjamin [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-12-09

    There are no very detailed descriptions of most of these instruments in the literature – we will attempt to fix that problem in the future. The BDD instruments are described in [1]. One of the dosimeter instruments on CXD boxes is described in [2]. These documents (or web links to them) and a few others are in this directory tree. The cross calibration of the CXD electron data with RBSP is described in [3]. Each row in the data file contains the data from one time bin from a CXD or BDD instrument along with a variety of parameters derived from the data. Time steps are commandable but 4 minutes is a typical setting. These instruments are on many (but not all) GPS satellites which are currently in operation. The data come from either BDD instruments on GPS Block IIR satellites (SVN41 and 48), or else CXD-IIR instruments on GPS Block IIR and IIR-M satellites (SVN53-61) or CXD-IIF instruments on GPS block IIF satellites (SVN62-73). The CXD-IIR instruments on block IIR and IIR(M) satellites use the same design.

  11. Archive of Census Related Products (ACRP): 1980 SAS Transport Files

    Data.gov (United States)

    National Aeronautics and Space Administration — The 1980 SAS Transport Files portion of the Archive of Census Related Products (ACRP) contains housing and population demographics from the 1980 Summary Tape File...

  12. 77 FR 45348 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-07-31

    ...: Operational Interface and Transfer of Asset Agreement with ANP Bellingham to be effective 9/22/2012. Filed... with ANP Blackstone to be effective 9/22/2012. Filed Date: 7/23/12 Accession Number: 20120723-5156...

  13. 77 FR 51991 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-08-28

    ... following electric corporate filings: Docket Numbers: EC12-135-000. Applicants: Energia Sierra Juarez U.S., LLC. Description: Section 203 Application of Energia Sierra Juarez U.S., LLC. Filed Date: 8/20/12...

  14. 5 CFR 831.1204 - Filing disability retirement applications: General.

    Science.gov (United States)

    2010-01-01

    ... business. The date of filing by commercial overnight delivery is the date the application is given to the... executed or submitted in a letter or other form not prescribed by OPM, is deemed timely filed. OPM will not...

  15. 77 FR 23707 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-04-20

    ... following exempt wholesale generator filings: Docket Numbers: EG12-56-000. Applicants: NRG Solar Alpine LLC. Description: NRG Solar Alpine, LLC Notice of Self Certification of Exempt Wholesale Generator Status. Filed...

  16. 76 FR 14964 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-03-18

    ... following exempt wholesale generator filings: Docket Numbers: EG11-68-000. Applicants: FRV AE Solar, LLC. Description: Notice of Self-Certification as an Exempt Wholesale Generator by FC of FRV AE Solar, LLC. Filed...

  17. 75 FR 57751 - Combined Notice of Filings # 1

    Science.gov (United States)

    2010-09-22

    .... Applicants: Waterbury Generation, LLC. Description: Waterbury Generation, LLC submits tariff filing per 35.12: Waterbury Generation, LLC Baseline Tariff to be effective 9/14/ 2010. Filed Date: 09/14/2010. Accession...

  18. 76 FR 12353 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-03-07

    ...-630-000. Applicants: Heritage Hospital. Description: Amending initial self-certification. Filed Date... Food Stores, Inc. Description: Form 556 of Lowe's Food Stores, Inc. Filed Date: 01/11/2011. Accession...

  19. 75 FR 3721 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-01-22

    ... Bay Cable LLC. Description: Supplemental Filing to Application of Trans Bay Cable LLC. Filed Date: 01...; FPL Energy Green Power Wind, LLC; FPL Energy Hancock County Wind, LLC; FPLE Maine Hydro, LLC; FPL...

  20. 77 FR 10737 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-02-23

    ... following electric rate filings: Docket Numbers: ER12-21-003. Applicants: Agua Caliente Solar, LLC. Description: Notice of Change in Status of Agua Caliente Solar, LLC. Filed Date: 2/14/12. Accession Number...

  1. 77 FR 3759 - Combined Notice of Filings #2

    Science.gov (United States)

    2012-01-25

    ... following electric rate filings: Docket Numbers: ER12-21-002. Applicants: Agua Caliente Solar, LLC. Description: Clarification of Notice of Change in Status for Agua Caliente. Filed Date: 1/13/12. Accession...

  2. Fair Housing Act Cases Filed by Year and State

    Data.gov (United States)

    Department of Housing and Urban Development — The 'Fair Housing Act Cases Filed by Year and State with the Bases and Outcomes - 2000-2013' dataset summarizes the cases filed as a result of complaints made...

  3. 75 FR 65317 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-10-22

    ... Numbers: ER11-85-000. Applicants: Societe Generale Energie (USA) Corp. Description: Societe Generale Energie (USA) Corp. submits tariff filing per 35.12: SGE USA Baseline Tariff Filing to be effective 10/12...

  4. Supplemental Security Income Public-Use Microdata File, 2001 Data

    Data.gov (United States)

    Social Security Administration — The SSI Public-Use Microdata File contains an extract of data fields from SSA's Supplemental Security Record file and consists of a 5 percent random, representative...

  5. 14 CFR 303.03 - Requirement to file application.

    Science.gov (United States)

    2010-01-01

    ... PROCEEDINGS) PROCEDURAL REGULATIONS REVIEW OF AIR CARRIER AGREEMENTS General Provisions § 303.03 Requirement to file application. A person who seeks approval of a section 412 transaction must file with the...

  6. 76 FR 36107 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-06-21

    ... Light Company submits tariff filing per 35.17(b): IPL Changes in Depreciation Rates for Wholesale... tariff filing per 35.13(a)(2)(iii: IPL & Lakefield Wind Project--LBA Agreement to be effective 6/15/2011...

  7. 76 FR 46791 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-08-03

    ... submits tariff filing per 35.17(b): IPL & Elk Wind Energy--LBA Agreement Amendment to be effective 6/14.... Description: Interstate Power and Light Company submits tariff filing per 35.17(b): IPL & Lakefield Wind...

  8. FDIC Institution Directory (ID) -- Insured Insitution Download File

    Data.gov (United States)

    Federal Deposit Insurance Corporation — The FDIC's Institution Directory (ID) download file provides a list of all FDIC-insured institutions. The file includes demographic information related to the...

  9. Are we married? State tax filing problems after Windsor

    National Research Council Canada - National Science Library

    Bernstein, Aaron M

    2015-01-01

    .... A same-sex spouse residing in a state that does not recognize same-sex marriage is required to file federal taxes as married under federal law but is prohibited from filing as married in-state...

  10. THE USELESS OF THE TRANSFER PRICING FILE

    Directory of Open Access Journals (Sweden)

    DEACONU SORIN-CONSTANTIN

    2017-10-01

    Full Text Available In the context of the globalization and of the strengthening of the relations between the UE economical agents, it appears more and more the idea of groups of entities for the justification of the reciprocal deliveries, buying and loans. The transferring price is a concept that requires maximum attention from the affiliated parts, meaning the entities with reciprocal operations and common managers or associates. The theme of our study is the verification of the hypothesis when the transferring price documentary file is not useful. The research methodology of our study include: dedicated literature, study cases, etc. the conclusion is that all affiliated entities (according to the national legislation are legally imposed to establish such a documentary file and the tax authorities may require explanations and information related to the content of this file. Still, the file does not bring extra information for the tax authorities, because its establishing is partial from the start, because no tax payer will accuse himself in front of the tax authorities. We propose the elimination of this documentary file and its replacement with an electronic format of information to an international level, so the tax authority can perform an analysis of the intra-group operation without going to the premises of the economical operators

  11. DIRAC File Replica and Metadata Catalog

    Science.gov (United States)

    Tsaregorodtsev, A.; Poss, S.

    2012-12-01

    File replica and metadata catalogs are essential parts of any distributed data management system, which are largely determining its functionality and performance. A new File Catalog (DFC) was developed in the framework of the DIRAC Project that combines both replica and metadata catalog functionality. The DFC design is based on the practical experience with the data management system of the LHCb Collaboration. It is optimized for the most common patterns of the catalog usage in order to achieve maximum performance from the user perspective. The DFC supports bulk operations for replica queries and allows quick analysis of the storage usage globally and for each Storage Element separately. It supports flexible ACL rules with plug-ins for various policies that can be adopted by a particular community. The DFC catalog allows to store various types of metadata associated with files and directories and to perform efficient queries for the data based on complex metadata combinations. Definition of file ancestor-descendent relation chains is also possible. The DFC catalog is implemented in the general DIRAC distributed computing framework following the standard grid security architecture. In this paper we describe the design of the DFC and its implementation details. The performance measurements are compared with other grid file catalog implementations. The experience of the DFC Catalog usage in the CLIC detector project are discussed.

  12. Analyzing endosonic root canal file oscillations: an in vitro evaluation.

    Science.gov (United States)

    Lea, Simon C; Walmsley, A Damien; Lumley, Philip J

    2010-05-01

    Passive ultrasonic irrigation may be used to improve bacterial reduction within the root canal. The technique relies on a small file being driven to oscillate freely within the canal and activating an irrigant solution through biophysical forces such as microstreaming. There is limited information regarding a file's oscillation patterns when operated while surrounded by fluid as is the case within a canal root. Files of different sizes (#10 and #30, 27 mm and 31 mm) were connected to an ultrasound generator via a 120 degrees file holder. Files were immersed in a water bath, and a laser vibrometer set up with measurement lines superimposed over the files. The laser vibrometer was scanned over the oscillating files. Measurements were repeated 10 times for each file/power setting used. File mode shapes are comprised of a series of nodes/antinodes, with thinner, longer files producing more antinodes. The maximum vibration occurred at the free end of the file. Increasing generator power had no significant effect on this maximum amplitude (p > 0.20). Maximum displacement amplitudes were 17 to 22 microm (#10 file, 27 mm), 15 to 21 microm (#10 file, 31 mm), 6 to 9 microm (#30 file, 27 mm), and 5 to 7 microm (#30, 31 mm) for all power settings. Antinodes occurring along the remaining file length were significantly larger at generator power 1 than at powers 2 through 5 (p < 0.03). At higher generator powers, energy delivered to the file is dissipated in unwanted vibration resulting in reduced vibration displacement amplitudes. This may reduce the occurrence of the biophysical forces necessary to maximize the technique's effectiveness. Copyright (c) 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  13. A Pragmatist's Approach to Creating a Private File.

    Science.gov (United States)

    Tchobanoff, James B.

    1985-01-01

    Describes experiences involved in the development of a private file (software and hardware) at The Pillsbury Company over a period of approximately two decades. Evolution of the file, use of STAIRS (a sophisticated file-management system and search program), sample reports and entries, database structure, and online searching are highlighted. (EJS)

  14. 76 FR 69715 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-11-09

    ... the following electric corporate filings: Docket Numbers: EC12-20-000. Applicants: Duke Energy Ohio... Application regarding Huxley Substation Transmission Assets. Filed Date: 11/02/2011. Accession Number... following electric rate filings: Docket Numbers: ER10-1478-003. Applicants: Pennsylvania Electric Company...

  15. 78 FR 61942 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-10-07

    ... following electric corporate filings: Docket Numbers: EC13-155-000. Applicants: Consolidated Edison Company... Commission received the following electric rate filings: Docket Numbers: ER10-1847-004; ER10-1856-004; ER10... 1 Round Valley Substation to be effective 10/1/2013. Filed Date: 9/26/13. Accession Number: 20130926...

  16. 76 FR 65713 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-10-24

    ... following electric corporate filings: Docket Numbers: EC12-8-000. Applicants: Plymouth Rock Energy, LLC. Description: Application for Approval Under Section 203 of Plymouth Rock Energy, LLC. Filed Date: 10/13/2011... Service Company of New Mexico. Description: Public Service Company of New Mexico submits tariff filing per...

  17. 75 FR 49918 - Combined Notice of Filings # 1

    Science.gov (United States)

    2010-08-16

    ... LLC. Description: Grays Harbor Energy LLC submits tariff filing per 35: Refile Baseline Market-Based.... Description: Kleen Energy Systems, LLC submits captioned public utility's conformed market-based rate tariff... per 35: Refile Baseline Market-Based Rate Tariff Filing to be effective 8/5/ 2010. Filed Date: 08/05...

  18. 75 FR 49923 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-08-16

    ...): Supplement to Initial Market-Based Rate Application to be effective 11/10/2010. Filed Date: 08/02/2010... Filing (Market-Based Volume 28) to be effective 8/2/2010. Filed Date: 08/02/2010. Accession Number...-2108-000. Applicants: Heritage Stoney Corners Wind Farm I, LLC. Description: Gray PLLC submits an...

  19. 78 FR 16848 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-03-19

    ... 3/29/13. Take notice that the Commission received the following exempt wholesale generator filings...-Certification of Exempt Wholesale Generator Status of Goldthwaite Wind Energy LLC. Filed Date: 3/7/13. Accession... Solar 1, LLC. Description: Copper Mountain Solar 1, LLC submits tariff filing per 35.13(a)(2)(iii...

  20. 48 CFR 750.7109-1 - Filing requests.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Filing requests. 750.7109... Policy Interests of the United States 750.7109-1 Filing requests. Any person (hereinafter called the “contractor”) seeking an adjustment under standards set forth in 750.7106 may file a request in duplicate with...

  1. 75 FR 63452 - Combined Notice Of Filings #1

    Science.gov (United States)

    2010-10-15

    ... Ridge Energy LLC submits tariff filing per 35.12: Baseline Filing of Assignment, Co-Tenancy and Shared... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice Of Filings 1 October 6, 2010. Take notice that the...

  2. 77 FR 2935 - Mars, Inc.; Filing of Color Additive Petition

    Science.gov (United States)

    2012-01-20

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 73 Mars, Inc.; Filing of Color Additive Petition... Administration (FDA) is announcing that Mars, Inc., has filed a petition proposing that the color additive... additive petition (CAP 2C0293) has been filed by Mars, Inc., c/o Keller and Heckman LLP, 1001 G St. NW...

  3. 7 CFR 51.26 - Record of filing time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Record of filing time. 51.26 Section 51.26 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Appeal Inspection § 51.26 Record of filing time. A record showing the date and time of filing an...

  4. 33 CFR 52.22 - Time limit for filing application.

    Science.gov (United States)

    2010-07-01

    ... PERSONNEL BOARD FOR CORRECTION OF MILITARY RECORDS OF THE COAST GUARD General Provisions Regarding Applications § 52.22 Time limit for filing application. An application for correction of a record must be filed... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Time limit for filing application...

  5. 77 FR 48133 - Combined Notice of Filings #2

    Science.gov (United States)

    2012-08-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 2 Take notice that the Commission received the... Certificate of Concurrence Compliance Filing to be effective 7/1/2012. Filed Date: 7/31/12. Accession Number...

  6. Digital Libraries: The Next Generation in File System Technology.

    Science.gov (United States)

    Bowman, Mic; Camargo, Bill

    1998-01-01

    Examines file sharing within corporations that use wide-area, distributed file systems. Applications and user interactions strongly suggest that the addition of services typically associated with digital libraries (content-based file location, strongly typed objects, representation of complex relationships between documents, and extrinsic…

  7. 45 CFR 34.3 - Filing procedures and time limits.

    Science.gov (United States)

    2010-10-01

    ... submitted under this part must be presented in writing to the Claims Officer (See paragraph (c) of this... has been filed and/or paid; (6) Travel orders, if applicable; (7) Any citations or traffic tickets, if...) A claim filed under this section must be filed in writing with the Department within two years from...

  8. 27 CFR 31.171 - Method of filing.

    Science.gov (United States)

    2010-04-01

    ...) Supporting documents for receipts and dispositions, such as delivery receipts and bills of lading, also may...' Records and Reports § 31.171 Method of filing. A wholesale dealer may file the records of receipt and... records of receipt and disposition in accordance with their own filing system as long as the records are...

  9. 43 CFR 36.4 - Filing of application.

    Science.gov (United States)

    2010-10-01

    ... ACROSS, AND ACCESS INTO, CONSERVATION SYSTEM UNITS IN ALASKA § 36.4 Filing of application. (a) A SF 299... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Filing of application. 36.4 Section 36.4...) Prior to filing the SF 299, the applicant shall determine whether additional information to that...

  10. 40 CFR 305.8 - Examination of documents filed.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 27 2010-07-01 2010-07-01 false Examination of documents filed. 305.8... Examination of documents filed. (a) Inspection of Documents. Subject to the provisions of law restricting... copy any document filed in any proceeding. Such documents shall be made available by the Claims...

  11. 12 CFR Appendix F to Part 360 - Customer File Structure

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Customer File Structure F Appendix F to Part... POLICY RESOLUTION AND RECEIVERSHIP RULES Pt. 360, App. F Appendix F to Part 360—Customer File Structure This is the structure of the data file to provide to the FDIC information related to each customer who...

  12. 76 FR 24467 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-05-02

    ... following electric corporate filings: Docket Numbers: EC11-74-000. Applicants: Macho Springs Power I, LLC... per Order in ER11-2745-000 to resubmit the Schedule A Image to be effective 12/20/2010. Filed Date: 04... Schedule A Image to be effective 12/28/2010. Filed Date: 04/25/2011. Accession Number: 20110425-5170...

  13. 10 CFR 1003.71 - Who may file.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Who may file. 1003.71 Section 1003.71 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICE OF HEARINGS AND APPEALS PROCEDURAL REGULATIONS Private Grievances and Redress § 1003.71 Who may file. Any person may file a petition under this subpart who is adversely...

  14. 43 CFR 4.1150 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1150 Section 4.1150 Public Lands: Interior Office of the Secretary of the Interior DEPARTMENT HEARINGS AND APPEALS PROCEDURES... Assessments of Civil Penalties § 4.1150 Who may file. Any person charged with a civil penalty may file a...

  15. 46 CFR 550.401 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Who may file. 550.401 Section 550.401 Shipping FEDERAL... Petitions for Section 19 Relief § 550.401 Who may file. Any person who has been harmed by, or who can... the United States, may file a petition for relief under the provisions of this part. ...

  16. 43 CFR 4.1290 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1290 Section 4.1290 Public Lands: Interior Office of the Secretary of the Interior DEPARTMENT HEARINGS AND APPEALS PROCEDURES... Expenses Under Section 525(e) of the Act § 4.1290 Who may file. (a) Any person may file a petition for...

  17. 29 CFR 2700.40 - Who may file.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Who may file. 2700.40 Section 2700.40 Labor Regulations... of Discharge, Discrimination or Interference § 2700.40 Who may file. (a) The Secretary. A...), may be filed by the complaining miner, representative of miners, or applicant for employment if the...

  18. 10 CFR 1003.31 - Who may file.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Who may file. 1003.31 Section 1003.31 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICE OF HEARINGS AND APPEALS PROCEDURAL REGULATIONS Appeals § 1003.31 Who may file. Any person may file an appeal under this subpart who is so authorized by § 1003.27, a...

  19. 76 FR 41774 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-07-15

    ...: Bruce Power Inc. Description: Bruce Power Inc. submits tariff filing per 35.37: Bruce Power Inc... Time on Thursday, July 21, 2011. Docket Numbers: ER11-3968-000. Applicants: Duke Energy Lee II, LLC. Description: Duke Energy Lee II, LLC submits tariff filing per 35.37: Triennial Filing to be effective 1/9...

  20. 76 FR 21721 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-04-18

    ... Filing to Submit Revised Market-Based Rate Tariff to be effective 1/14/2011 under ER11-2882-001 Filing...)(iii: GenOn Diesels Distribution Service Agreement-- Amendment to First Supplement to be effective 6/1.... Applicants: Sempra Generation. Description: Sempra Generation submits tariff filing per 35.13(a)(2)(iii...

  1. 78 FR 46850 - Filing Financial and Other Reports

    Science.gov (United States)

    2013-08-02

    ... ADMINISTRATION 12 CFR Parts 741 and 748 RIN 3313-AE25 Filing Financial and Other Reports AGENCY: National Credit... its regulations regarding filing financial, statistical, and other reports and credit union profiles... Proposed Rulemaking for Parts 741 and 748, Filing financial and other reports'' in the email subject line...

  2. 75 FR 61718 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-10-06

    ... & Deep Creek LLC. Description: Brookfield Power Piney & Deep Creek LLC submits tariff filing per 35.12: Brookfield Power Piney & Deep Creek LLC Baseline Market-Based Rate Tariff to be effective 9/24/2010. Filed... service, persons with Internet access who will eFile a document and/or be listed as a contact for an...

  3. 76 FR 46790 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-08-03

    ... following electric rate filings: Docket Numbers: ER10-78-003. Applicants: Orange Grove Energy, L.P... in Status Orange Grove Energy, L.P. Filed Date: 07/14/2011. Accession Number: 20110714-5038. Comment... submits tariff filing per 35.12: Metering Agent Agreement between WPPI, L'Anse & UPPCO to be effective 10...

  4. FRAMES User Defined Body Burden Concentration File Module Documentation

    Energy Technology Data Exchange (ETDEWEB)

    Pelton, Mitchell A.; Rutz, Frederick C.; Eslinger, Melany A.; Gelston, Gariann M.

    2001-06-01

    The Framework for Risk Analysis in Multimedia Environmental Systems (FRAMES) Body Burden Concentration File (BBF) contains time-varying, instantaneous, constituent concentrations for body burden by contaminant. This report contains the requirements for this file and will be used by software engineers and testers to ensure that the file inputs properly.

  5. 19 CFR 191.0a - Claims filed under NAFTA.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Claims filed under NAFTA. 191.0a Section 191.0a Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) DRAWBACK § 191.0a Claims filed under NAFTA. Claims for drawback filed under the...

  6. 77 FR 62505 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-10-15

    ... the following exempt wholesale generator filings: Docket Numbers: EG13-1-000. Applicants: Big Blue Wind Farm, LLC. Description: Self-Certification of EWG Status of Big Blue Wind Farm, LLC. Filed Date... and Related Requests of LDH Rensselaer LLC and Louis Dreyfus Energy Services, L.P. Filed Date: 10/3/12...

  7. 78 FR 52523 - Big Rivers Electric Corporation; Notice of Filing

    Science.gov (United States)

    2013-08-23

    ... Energy Regulatory Commission Big Rivers Electric Corporation; Notice of Filing Take notice that on August 16, 2013, Big Rivers Electric Corporation filed its proposed revenue requirements for reactive supply..., as appropriate. Such notices, motions, or protests must be filed on or before the comment date. On or...

  8. 77 FR 71410 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-11-30

    ... Act for Transfer of Jurisdictional Facilities and Requests for Waivers of Filing Requirements. Filed...-000. Applicants: Entergy Arkansas, Inc. Description: Chisholm View Wind Transfer Agreement to be... Participate in PJM Reliability Pricing Model Auctions. Filed Date: 11/15/12. Accession Number: 20121115-5174...

  9. 77 FR 34376 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-06-11

    ... Municipal Complex Cogeneration to be effective 6/1/2012. Filed Date: 6/1/12. Accession Number: 20120601-5260...: Market-Based Rate Application to be effective 8/1/ 2012. Filed Date: 6/1/12. Accession Number: 20120601... following electric corporate filings: Docket Numbers: EC12-106-000. Applicants: Arizona Public Service...

  10. 20 CFR 655.15 - Required pre-filing recruitment.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Required pre-filing recruitment. 655.15... States (H-2B Workers) § 655.15 Required pre-filing recruitment. (a) Time of filing of application. An... recruitment steps set forth in this section have been fully satisfied, except where specifically exempted from...

  11. 46 CFR 232.6 - Financial report filing requirement.

    Science.gov (United States)

    2010-10-01

    ... RELATED ACTIVITIES UNIFORM FINANCIAL REPORTING REQUIREMENTS Income Statement § 232.6 Financial report filing requirement. (a) Reporting Frequency and Due Dates. The contractor shall file a semiannual... 46 Shipping 8 2010-10-01 2010-10-01 false Financial report filing requirement. 232.6 Section 232.6...

  12. 75 FR 62378 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-10-08

    ... Filings 1 September 30, 2010. Take notice that the Commission received the following electric corporate...: Power Receivable Finance, LLC. Description: Power Receivable Finance, LLC submits tariff filing per 35.12: Power Receivable Finance, LLC MBR Tariff to be effective 9/ 29/2010. Filed Date: 09/29/2010...

  13. 75 FR 70733 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-11-18

    ... Time on Friday, November 19, 2010. Docket Numbers: ER11-1966-000. Applicants: Verde Energy USA Inc. Description: Verde Energy USA Inc. submits tariff filing per 35.12: Baseline Filing to be effective 10/29/2010...: Alcan Power Marketing, Inc. Description: Alcan Power Marketing, Inc. submits tariff filing per 35.12...

  14. 76 FR 17642 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-03-30

    ... Central Company. Description: AEP Texas Central Company submits tariff filing per 35.15: 20110322 TCC...): 20110322 TCC-Magic Valley GIA to be effective 2/25/ 2011. Filed Date: 03/22/2011. Accession Number...(a)(2)(iii): 20110322 TCC-Los Vientos GIA to be effective 3/7/ 2011. Filed Date: 03/22/2011...

  15. 75 FR 74034 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-11-30

    ...: Sunoco Power Marketing, LLC. Description: Sunoco Power Marketing, LLC submits tariff filing per 35: Sunoco Power Marketing Baseline Filing to be effective 11/18/2010. Filed Date: 11/18/2010. Accession...-001. Applicants: Delmarva Power & Light Company. Description: Delmarva Power & Light Company submits...

  16. 21 CFR 720.1 - Who should file.

    Science.gov (United States)

    2010-04-01

    ... VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.1 Who should file. Either the manufacturer, packer, or distributor of a cosmetic product is requested to file Form FDA 2512 (“Cosmetic Product Ingredient Statement”), whether or not the cosmetic product enters interstate commerce. This...

  17. 21 CFR 720.2 - Times for filing.

    Science.gov (United States)

    2010-04-01

    ... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) COSMETICS VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.2 Times for filing. Within 180 days after forms are made available to the industry, Form FDA 2512 should be filed for each cosmetic...

  18. 75 FR 70736 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-11-18

    ... Company. Description: Interstate Power and Light Company submits tariff filing per 35.13(a)(2)(iii): IPL... filing per 35.13(a)(2)(iii): IPL WPL--LBA Agreement to be effective 12/ 28/2010. Filed Date: 10/29/2010...

  19. 76 FR 42704 - Sky River LLC; Notice of Filing

    Science.gov (United States)

    2011-07-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Sky River LLC; Notice of Filing Take notice that, on July 8, 2011, Sky River LLC filed to amend its Open Access Transmission Tariff (OATT) filing, submitted on April 1, 2011 and...

  20. Defining File Format Obsolescence: A Risky Journey

    Directory of Open Access Journals (Sweden)

    David Pearson

    2008-08-01

    Full Text Available File format obsolescence is a major risk factor threatening the ongoing usefulness of digital information collections. While the preservation community has become increasingly interested in tools for assessing a wide range of risks, the National Library of Australia is developing mechanisms specifically focused on the risks of format obsolescence. The paper reports on the AONS II Project, undertaken in conjunction with the Australian Partnership for Sustainable Repositories (APSR. The project aimed to refine and develop a software tool that would automatically find and report indicators of obsolescence risks, to help repository managers decide if preservation action is needed. The paper discusses the current mismatch between this objective and the available sources of information on file formats, and emphasises the need to take account of both local and global factors in assessing risk. The paper calls for the preservation community to engage with the further development of thinking about file format obsolescence.

  1. ESUSA: U. S. endangered species distribution file

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, J; Calef, C E

    1978-05-01

    A file containing distribution data on federally listed or proposed endangered species of the United States is described. Included are (a) the common name, (b) the scientific name, (c) the taxonomic family, (d) the OES/FWS/USDI group (mammal, bird, etc.), (e) the status, (f) the geographic distribution by counties, and (g) Federal Register references. Status types are endangered, threatened, proposed, under review, deleted, and rejected. Distribution is by Federal Information Processing Standard (FIPS) county code and is of four types: designated critical habitat, present range, potential range, and historic range. The file is currently being used in conjunction with similar data on projected future energy facilities to anticipate possible conflicts. However, the file would be useful to any project correlating endangered species with location information expressed by county. An example is as an aid in evaluating Forest Service or Bureau of Land Management proposed wilderness areas.

  2. Auto Draw from Excel Input Files

    Science.gov (United States)

    Strauss, Karl F.; Goullioud, Renaud; Cox, Brian; Grimes, James M.

    2011-01-01

    The design process often involves the use of Excel files during project development. To facilitate communications of the information in the Excel files, drawings are often generated. During the design process, the Excel files are updated often to reflect new input. The problem is that the drawings often lag the updates, often leading to confusion of the current state of the design. The use of this program allows visualization of complex data in a format that is more easily understandable than pages of numbers. Because the graphical output can be updated automatically, the manual labor of diagram drawing can be eliminated. The more frequent update of system diagrams can reduce confusion and reduce errors and is likely to uncover symmetric problems earlier in the design cycle, thus reducing rework and redesign.

  3. Tuning HDF5 for Lustre File Systems

    Energy Technology Data Exchange (ETDEWEB)

    Howison, Mark; Koziol, Quincey; Knaak, David; Mainzer, John; Shalf, John

    2010-09-24

    HDF5 is a cross-platform parallel I/O library that is used by a wide variety of HPC applications for the flexibility of its hierarchical object-database representation of scientific data. We describe our recent work to optimize the performance of the HDF5 and MPI-IO libraries for the Lustre parallel file system. We selected three different HPC applications to represent the diverse range of I/O requirements, and measured their performance on three different systems to demonstrate the robustness of our optimizations across different file system configurations and to validate our optimization strategy. We demonstrate that the combined optimizations improve HDF5 parallel I/O performance by up to 33 times in some cases running close to the achievable peak performance of the underlying file system and demonstrate scalable performance up to 40,960-way concurrency.

  4. Next generation WLCG File Transfer Service (FTS)

    CERN Multimedia

    CERN. Geneva

    2012-01-01

    LHC experiments at CERN and worldwide utilize WLCG resources and middleware components to perform distributed computing tasks. One of the most important tasks is reliable file replication. It is a complex problem, suffering from transfer failures, disconnections, transfer duplication, server and network overload, differences in storage systems, etc. To address these problems, EMI and gLite have provided the independent File Transfer Service (FTS) and Grid File Access Library (GFAL) tools. Their development started almost a decade ago, in the meantime, requirements in data management have changed - the old architecture of FTS and GFAL cannot keep support easily these changes. Technology has also been progressing: FTS and GFAL do not fit into the new paradigms (cloud, messaging, for example). To be able to serve the next stage of LHC data collecting (from 2013), we need a new generation of  these tools: FTS 3 and GFAL 2. We envision a service requiring minimal configuration, which can dynamically adapt to the...

  5. The Future of the Andrew File System

    CERN Multimedia

    CERN. Geneva; Altman, Jeffrey

    2011-01-01

    The talk will discuss the ten operational capabilities that have made AFS unique in the distributed file system space and how these capabilities are being expanded upon to meet the needs of the 21st century. Derrick Brashear and Jeffrey Altman will present a technical road map of new features and technical innovations that are under development by the OpenAFS community and Your File System, Inc. funded by a U.S. Department of Energy Small Business Innovative Research grant. The talk will end with a comparison of AFS to its modern days competitors.

  6. An evaluated neutronic data file for bismuth

    Energy Technology Data Exchange (ETDEWEB)

    Guenther, P.T.; Lawson, R.D.; Meadows, J.W.; Smith, A.B.; Smith, D.L.; Sugimoto, M. (Argonne National Lab., IL (USA)); Howerton, R.J. (Lawrence Livermore National Lab., CA (USA))

    1989-11-01

    A comprehensive evaluated neutronic data file for bismuth, extending from 10{sup {minus}5} eV to 20.0 MeV, is described. The experimental database, the application of the theoretical models, and the evaluation rationale are outlined. Attention is given to uncertainty specification, and comparisons are made with the prior ENDF/B-V evaluation. The corresponding numerical file, in ENDF/B-VI format, has been transmitted to the National Nuclear Data Center, Brookhaven National Laboratory. 106 refs., 10 figs., 6 tabs.

  7. 78 FR 26768 - Great Lakes Hydro America, LLC; Notice of Intent To File License Application, Filing of Pre...

    Science.gov (United States)

    2013-05-08

    ... Energy Regulatory Commission Great Lakes Hydro America, LLC; Notice of Intent To File License Application...-filing Process. b. Project No.: 2520-072. c. Dated Filed: March 1, 2013. d. Submitted By: Great Lakes... designating Great Lakes Hydro America, LLC as the Commission's non-federal representative for carrying out...

  8. 75 FR 39224 - PK Ventures, Inc.; Notice of Intent To File License Application, Filing of Pre-Application...

    Science.gov (United States)

    2010-07-08

    ... Energy Regulatory Commission PK Ventures, Inc.; Notice of Intent To File License Application, Filing of... Licensing Process. b. Project No.: 4093-031. c. Dated Filed: April 30, 2010. d. Submitted By: PK Ventures....com. i. FERC Contact: Sean Murphy at (202) 502-6145; or e-mail at [email protected] . j. PK...

  9. 78 FR 59923 - Cave Run Energy, LLC; Notice of Intent To File License Application, Filing of Pre-Application...

    Science.gov (United States)

    2013-09-30

    ... Energy Regulatory Commission Cave Run Energy, LLC; Notice of Intent To File License Application, Filing.... Project No.: 14376-001. c. Date Filed: July 21, 2013. d. Submitted By: Cave Run Energy, LLC. e. Name of Project: Cave Run Hydroelectric Project. f. Location: On the Licking River, in Rowan and Bath counties...

  10. Text Classification: Classifying Plain Source Files with Neural Network

    Directory of Open Access Journals (Sweden)

    Jaromir Veber

    2010-10-01

    Full Text Available The automated text file categorization has an important place in computer engineering, particularly in the process called data management automation. A lot has been written about text classification and the methods allowing classification of these files are well known. Unfortunately most studies are theoretical and for practical implementation more research is needed. I decided to contribute with a research focused on creating of a classifier for different kinds of programs (source files, scripts…. This paper will describe practical implementation of the classifier for text files depending on file content.

  11. Design and Implementation of a Metadata-rich File System

    Energy Technology Data Exchange (ETDEWEB)

    Ames, S; Gokhale, M B; Maltzahn, C

    2010-01-19

    Despite continual improvements in the performance and reliability of large scale file systems, the management of user-defined file system metadata has changed little in the past decade. The mismatch between the size and complexity of large scale data stores and their ability to organize and query their metadata has led to a de facto standard in which raw data is stored in traditional file systems, while related, application-specific metadata is stored in relational databases. This separation of data and semantic metadata requires considerable effort to maintain consistency and can result in complex, slow, and inflexible system operation. To address these problems, we have developed the Quasar File System (QFS), a metadata-rich file system in which files, user-defined attributes, and file relationships are all first class objects. In contrast to hierarchical file systems and relational databases, QFS defines a graph data model composed of files and their relationships. QFS incorporates Quasar, an XPATH-extended query language for searching the file system. Results from our QFS prototype show the effectiveness of this approach. Compared to the de facto standard, the QFS prototype shows superior ingest performance and comparable query performance on user metadata-intensive operations and superior performance on normal file metadata operations.

  12. Grammar-Based Specification and Parsing of Binary File Formats

    Directory of Open Access Journals (Sweden)

    William Underwood

    2012-03-01

    Full Text Available The capability to validate and view or play binary file formats, as well as to convert binary file formats to standard or current file formats, is critically important to the preservation of digital data and records. This paper describes the extension of context-free grammars from strings to binary files. Binary files are arrays of data types, such as long and short integers, floating-point numbers and pointers, as well as characters. The concept of an attribute grammar is extended to these context-free array grammars. This attribute grammar has been used to define a number of chunk-based and directory-based binary file formats. A parser generator has been used with some of these grammars to generate syntax checkers (recognizers for validating binary file formats. Among the potential benefits of an attribute grammar-based approach to specification and parsing of binary file formats is that attribute grammars not only support format validation, but support generation of error messages during validation of format, validation of semantic constraints, attribute value extraction (characterization, generation of viewers or players for file formats, and conversion to current or standard file formats. The significance of these results is that with these extensions to core computer science concepts, traditional parser/compiler technologies can potentially be used as a part of a general, cost effective curation strategy for binary file formats.

  13. 78 FR 21364 - Notice of Agreements Filed

    Science.gov (United States)

    2013-04-10

    ... removes Hyundai Merchant Marine Co., Ltd from the agreement. Agreement No.: 012199. Title: NYK/Hanjin... Co., Ltd.; and Hyundai Merchant Marine Co., Ltd. Filing Party: Jacob K. Lee; NYK Line (North America... as one Party); Hapag-Lloyd AG; Hyundai Merchant Marine Co., Ltd.; Mitsui O.S.K. Lines, Ltd.; Nippon...

  14. 77 FR 60433 - Notice of Agreements Filed

    Science.gov (United States)

    2012-10-03

    ... Express, Inc. and Great White Fleet Liner Services Ltd. Filing Party: Wade S. Hooker, Esq., 211 Central... principal office of Great White Fleet and Crowley Latin American Services. Agreement No.: 012055-002. Title... of the parties. Agreement No.: 012178-001. Title: GWF/Crowley Space Charter Agreement. Parties: Great...

  15. 10 CFR 431.423 - Filing requirements.

    Science.gov (United States)

    2010-01-01

    ... Efficiency and Renewable Energy, U.S. Department of Energy, Section 327 Petitions, Building Technologies, EE... 10 Energy 3 2010-01-01 2010-01-01 false Filing requirements. 431.423 Section 431.423 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY EFFICIENCY PROGRAM FOR CERTAIN COMMERCIAL AND INDUSTRIAL...

  16. 21 CFR 820.198 - Complaint files.

    Science.gov (United States)

    2010-04-01

    ... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES QUALITY SYSTEM REGULATION Records § 820.198 Complaint files. (a) Each manufacturer shall maintain... reported to FDA under part 803 of this chapter, Medical Device Reporting. (b) Each manufacturer shall...

  17. 12 CFR 303.161 - Filing procedures.

    Science.gov (United States)

    2010-01-01

    ... filed in letter form with the appropriate FDIC office at the same time as required conversion... letter form with the appropriate FDIC office as soon as practicable after adoption of its plan of...; (2) Certified board resolutions relating to the conversion; (3) A business plan, including a detailed...

  18. 76 FR 6606 - Combined Notice of Filings

    Science.gov (United States)

    2011-02-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal.... Comment Date: 5 p.m. Eastern Time on Wednesday, February 09, 2011. Docket Numbers: RP11-1732-000... filing per 154.204: J-W 34689 to Q-West Capacity Release Negative Rate Agreement to be effective 2/1/2011...

  19. 75 FR 74732 - Notice of Agreements Filed

    Science.gov (United States)

    2010-12-01

    ... Services Limited, Inc.; Mediterranean Shipping Company, SA; Seaboard Marine Ltd.; South Pacific Shipping Company, Ltd. (dba Ecuadorian Line); and Trinity Shipping Line. Filing Party: Wayne R. Rohde, Esq.; Cozen... APL's corporate address. Agreement No.: 012086-001. Title: Maersk Line/Horizon Lines Space Charter...

  20. 75 FR 20849 - Notice of Agreements Filed

    Science.gov (United States)

    2010-04-21

    ... Abaco Shipping Company Limited. Filing Parties: Neal M. Mayer, Esq.; Hoppel, Mayer & Coleman; 1050... Latin America Services, LLC.; Dole Ocean Cargo Express; Great White Fleet; King Ocean Services Limited... geographic scope of the Agreement. Agreement No.: 011830-008. Title: Indamex Cross Space Charter, Sailing and...

  1. 78 FR 25443 - Notice of Agreements Filed

    Science.gov (United States)

    2013-05-01

    ..., Ltd.; Seafreight Line, Ltd.; Tropical Shipping and Construction Company Limited; and Zim Integrated.... Agreement No.: 012172-001. Title: Maersk Line/MSC Caribbean Space Charter Agreement. Parties: A.P. Moller-Maersk A/S trading under the name Maersk Line and Mediterranean Shipping Company S.A. Filing Party: Wayne...

  2. 21 CFR 211.198 - Complaint files.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Complaint files. 211.198 Section 211.198 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL CURRENT GOOD MANUFACTURING PRACTICE FOR FINISHED PHARMACEUTICALS Records and Reports § 211.198 Complaint...

  3. 77 FR 27450 - Combined Notice of Filings

    Science.gov (United States)

    2012-05-10

    ... Numbers: RP12-668-000. Applicants: WPX Energy Marketing, LLC, Williams Energy Resources LLC. Description: Joint Petition of WPX Energy Marketing, LLC, et al. for Limited Waiver of Capacity Release Regulations...: Gulf South Pipeline Company, LP. Description: Capacity Negotiated Rate Agreement filing--Chesapeake...

  4. Iron filings cement engineered soil mix

    Science.gov (United States)

    Bioretention cells are used in urban stormwater management to reduce storm surge and nutrient loss. The cells are filled with mixtures of soil, sand, compost, and other materials, and are underlain by a drain. The purpose of this study was to determine if iron filings would be a suitable filter for ...

  5. 75 FR 9635 - Notice of Informational Filing

    Science.gov (United States)

    2010-03-03

    ... permit field testing of the software-based dispatch system for low density lines identified as Track...). Specifically, the informational filing contains a description of the TrackAccess product and an operational... testing of the product on or about March 1, 2010, or as soon thereafter as practicable, contingent upon...

  6. Fast probabilistic file fingerprinting for big data

    NARCIS (Netherlands)

    Tretjakov, K.; Laur, S.; Smant, G.; Vilo, J.; Prins, J.C.P.

    2013-01-01

    Background: Biological data acquisition is raising new challenges, both in data analysis and handling. Not only is it proving hard to analyze the data at the rate it is generated today, but simply reading and transferring data files can be prohibitively slow due to their size. This primarily

  7. 27 CFR 26.275 - Filing.

    Science.gov (United States)

    2010-04-01

    ...' invoices, delivery receipts, bills or lading, etc., or exact copies of the same, may be filed in accordance... importer conducts wholesale operations, one legible copy of each required record of receipt and disposition... conducts only retail operations, either loose-leaf or book records may be maintained for the daily receipt...

  8. 27 CFR 27.136 - Filing.

    Science.gov (United States)

    2010-04-01

    ... either loose-leaf or book records of the daily receipt of liquors which contain all the required information. (d) Supporting documents, such as consignors' invoices, delivery receipts, bills of lading, etc... copy of each required record of receipt and disposition shall be filed not later than one business day...

  9. Single-file water in nanopores.

    Science.gov (United States)

    Köfinger, Jürgen; Hummer, Gerhard; Dellago, Christoph

    2011-09-14

    Water molecules confined to pores with sub-nanometre diameters form single-file hydrogen-bonded chains. In such nanoscale confinement, water has unusual physical properties that are exploited in biology and hold promise for a wide range of biomimetic and nanotechnological applications. The latter can be realized by carbon and boron nitride nanotubes which confine water in a relatively non-specific way and lend themselves to the study of intrinsic properties of single-file water. As a consequence of strong water-water hydrogen bonds, many characteristics of single-file water are conserved in biological and synthetic pores despite differences in their atomistic structures. Charge transport and orientational order in water chains depend sensitively on and are mainly determined by electrostatic effects. Thus, mimicking functions of biological pores with apolar pores and corresponding external fields gives insight into the structure-function relation of biological pores and allows the development of technical applications beyond the molecular devices found in living systems. In this Perspective, we revisit results for single-file water in apolar pores, and examine the similarities and the differences between these simple systems and water in more complex pores. This journal is © the Owner Societies 2011

  10. One Quiz File, Several Modes of Delivery

    Science.gov (United States)

    Herbert, John C.

    2012-01-01

    This report offers online course designers, particularly those keen on using Moodle CMSs, a means of diversifying accessibility to their educational materials via multiple modes of delivery that do not require the creation of numerous files and formats for just one activity. The author has made contributions to the development of an open source…

  11. 75 FR 14159 - Notice of Agreements Filed

    Science.gov (United States)

    2010-03-24

    .... Agreement No.: 012085-001. Title: 2007 Crane Purchase, Relocation and Modification Agreement Between Matson..., Inc. Filing Party: Matthew Thomas, Esq.; Reed Smith LLP; 301 K Street, NW. Suite 1100-East Tower... agree on matters related to use of cranes by third parties. Agreement No.: 012093. Title: CSAV/K-Line...

  12. 24 CFR 17.67 - Claims files.

    Science.gov (United States)

    2010-04-01

    ... Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development... against whom a claim is pending in his office. The file shall be kept reasonably up to date by the... also contain a checklist or brief summary of actions taken to collect or comprise a claim. ...

  13. 78 FR 14779 - Combined Notice of Filings

    Science.gov (United States)

    2013-03-07

    ... and Sales Report of Colorado Interstate Gas Company LLC Filed Date: 8/31/12 Accession Number: 20120831... Numbers: RP13-612-000 Applicants: Dominion Cove Point LNG, LP Description: DCP--2013 Annual EPCA to be... Docket Numbers: RP13-613-000 Applicants: Dominion Cove Point LNG, LP Description: DCP--2013 Annual Fuel...

  14. 77 FR 47832 - Combined Notice of Filings

    Science.gov (United States)

    2012-08-10

    ... Numbers: RP10-996-000. Applicants: Dominion Cove Point LNG, LP. Description: DCP--2012 Report of Operational Sales and Purchase of Gas. Filed Date: 7/25/12. Accession Number: 20120725-5029. Comments Due: 5 p.... ET 8/13/12. Docket Numbers: RP12-909-000. Applicants: CenterPoint Energy Gas Transmission Comp...

  15. Physician Fee Schedule National Payment Amount File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The significant size of the Physician Fee Schedule Payment Amount File-National requires that database programs (e.g., Access, dBase, FoxPro, etc.) be used to read...

  16. 49 CFR 360.3 - Filing fees.

    Science.gov (United States)

    2010-10-01

    ... currency drawn upon funds deposited in a United States or foreign bank or other financial institution... separate proceedings. (e) Waiver or reduction of filing fees. It is the general policy of the Federal Motor... should be addressed to the Director, Office of Data Analysis and Information Systems. (ii) Basis. The...

  17. 75 FR 24702 - Notice of Agreements Filed

    Science.gov (United States)

    2010-05-05

    ... ( http://www.fmc.gov ) or by contacting the Office of Agreements at (202) 523-5793 or [email protected]fmc... Stevedores, Inc.; The Port of Philadelphia & Camden; and South Jersey Port Corporation. Filing Party: Francis... agreement updates and replaces the parties' previous agreement under FMC Agreement No. 008425. By Order of...

  18. SIDS-toADF File Mapping Manual

    Science.gov (United States)

    McCarthy, Douglas; Smith, Matthew; Poirier, Diane; Smith, Charles A. (Technical Monitor)

    2002-01-01

    The "CFD General Notation System" (CGNS) consists of a collection of conventions, and conforming software, for the storage and retrieval of Computational Fluid Dynamics (CFD) data. It facilitates the exchange of data between sites and applications, and helps stabilize the archiving of aerodynamic data. This effort was initiated in order to streamline the procedures in exchanging data and software between NASA and its customers, but the goal is to develop CGNS into a National Standard for the exchange of aerodynamic data. The CGNS development team is comprised of members from Boeing Commercial Airplane Group, NASA-Ames, NASA-Langley, NASA-Lewis, McDonnell-Douglas Corporation (now Boeing-St. Louis), Air Force-Wright Lab., and ICEM-CFD Engineering. The elements of CGNS address all activities associated with the storage of data on external media and its movement to and from application programs. These elements include: 1) The Advanced Data Format (ADF) Database manager, consisting of both a file format specification and its I/O software, which handles the actual reading and writing of data from and to external storage media; 2) The Standard Interface Data Structures (SIDS), which specify the intellectual content of CFD data and the conventions governing naming and terminology; 3) The SIDS-to-ADF File Mapping conventions, which specify the exact location where the CFD data defined by the SIDS is to be stored within the ADF file(s); and 4) The CGNS Mid-level Library, which provides CFD-knowledgeable routines suitable for direct installation into application codes. The SIDS-toADF File Mapping Manual specifies the exact manner in which, under CGNS conventions, CFD data structures (the SIDS) are to be stored in (i.e., mapped onto) the file structure provided by the database manager (ADF). The result is a conforming CGNS database. Adherence to the mapping conventions guarantees uniform meaning and location of CFD data within ADF files, and thereby allows the construction of

  19. FAST: FAST Analysis of Sequences Toolbox.

    Science.gov (United States)

    Lawrence, Travis J; Kauffman, Kyle T; Amrine, Katherine C H; Carper, Dana L; Lee, Raymond S; Becich, Peter J; Canales, Claudia J; Ardell, David H

    2015-01-01

    FAST (FAST Analysis of Sequences Toolbox) provides simple, powerful open source command-line tools to filter, transform, annotate and analyze biological sequence data. Modeled after the GNU (GNU's Not Unix) Textutils such as grep, cut, and tr, FAST tools such as fasgrep, fascut, and fastr make it easy to rapidly prototype expressive bioinformatic workflows in a compact and generic command vocabulary. Compact combinatorial encoding of data workflows with FAST commands can simplify the documentation and reproducibility of bioinformatic protocols, supporting better transparency in biological data science. Interface self-consistency and conformity with conventions of GNU, Matlab, Perl, BioPerl, R, and GenBank help make FAST easy and rewarding to learn. FAST automates numerical, taxonomic, and text-based sorting, selection and transformation of sequence records and alignment sites based on content, index ranges, descriptive tags, annotated features, and in-line calculated analytics, including composition and codon usage. Automated content- and feature-based extraction of sites and support for molecular population genetic statistics make FAST useful for molecular evolutionary analysis. FAST is portable, easy to install and secure thanks to the relative maturity of its Perl and BioPerl foundations, with stable releases posted to CPAN. Development as well as a publicly accessible Cookbook and Wiki are available on the FAST GitHub repository at https://github.com/tlawrence3/FAST. The default data exchange format in FAST is Multi-FastA (specifically, a restriction of BioPerl FastA format). Sanger and Illumina 1.8+ FastQ formatted files are also supported. FAST makes it easier for non-programmer biologists to interactively investigate and control biological data at the speed of thought.

  20. FAST: FAST Analysis of Sequences Toolbox

    Directory of Open Access Journals (Sweden)

    Travis J. Lawrence

    2015-05-01

    Full Text Available FAST (FAST Analysis of Sequences Toolbox provides simple, powerful open source command-line tools to filter, transform, annotate and analyze biological sequence data. Modeled after the GNU (GNU’s Not Unix Textutils such as grep, cut, and tr, FAST tools such as fasgrep, fascut, and fastr make it easy to rapidly prototype expressive bioinformatic workflows in a compact and generic command vocabulary. Compact combinatorial encoding of data workflows with FAST commands can simplify the documentation and reproducibility of bioinformatic protocols, supporting better transparency in biological data science. Interface self-consistency and conformity with conventions of GNU, Matlab, Perl, BioPerl, R and GenBank help make FAST easy and rewarding to learn. FAST automates numerical, taxonomic, and text-based sorting, selection and transformation of sequence records and alignment sites based on content, index ranges, descriptive tags, annotated features, and in-line calculated analytics, including composition and codon usage. Automated content- and feature-based extraction of sites and support for molecular population genetic statistics makes FAST useful for molecular evolutionary analysis. FAST is portable, easy to install and secure thanks to the relative maturity of its Perl and BioPerl foundations, with stable releases posted to CPAN. Development as well as a publicly accessible Cookbook and Wiki are available on the FAST GitHub repository at https://github.com/tlawrence3/FAST. The default data exchange format in FAST is Multi-FastA (specifically, a restriction of BioPerl FastA format. Sanger and Illumina 1.8+ FastQ formatted files are also supported. FAST makes it easier for non-programmer biologists to interactively investigate and control biological data at the speed of thought.

  1. 78 FR 77354 - Procedural Rules To Permit Parties To File and Serve Documents Electronically

    Science.gov (United States)

    2013-12-23

    ... HEALTH REVIEW COMMISSION 29 CFR Part 2700 Procedural Rules To Permit Parties To File and Serve Documents... permit parties to file and serve documents electronically. The Commission is permitting electronic filing... allow parties to file documents electronically with the Commission. Although parties may file documents...

  2. 77 FR 58821 - Alcoa Power Generating Inc.; Notice of Application Accepted for Filing, Soliciting Comments...

    Science.gov (United States)

    2012-09-24

    ... . If unable to be filed electronically, documents may be paper-filed. To paper-file, an original and..., using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp . You must include your name... Generating Inc. (APGI) filed a request for a temporary variance from the operating guidelines of their...

  3. Constructing AppleWorks Data Base Files for the Apple IIe Computer.

    Science.gov (United States)

    Schlenker, Richard M.

    This step-by-step guide to creating database files using the AppleWorks software on the Apple IIe computer covers (1) loading the program; (2) adding files to the desktop; (3) selecting the database option; (4) naming the file; (5) naming categories or fields; (6) inserting data; (7) changing database file formats; (8) altering the file layout;…

  4. 76 FR 62100 - Approval of Filing Fees for Exempt Reporting Advisers and Private Fund Advisers

    Science.gov (United States)

    2011-10-06

    ... equity fund assets would file Form PF on a quarterly basis. All other private fund advisers would file on... COMMISSION Approval of Filing Fees for Exempt Reporting Advisers and Private Fund Advisers AGENCY: Securities... filing Form ADV and private fund advisers filing Form PF. SUMMARY: The Securities and Exchange Commission...

  5. Single File Dynamics Advances with a Focus on Biophysical Relevance

    Science.gov (United States)

    Flomenbom, Ophir

    2014-08-01

    In this review (appearing in the Special Issue on single file dynamics in biophysics and related extensions), three recently treated variants in file dynamics are presented: files with density that is not fixed, files with heterogeneous particles, and files with slow particles. The results in these files include: • In files with a density law that is not fixed, but decays as a power law with an exponent a the distance from the origin, the particle in the origin mean square displacement (MSD) scales like MSD t[1+a]/2, with a Gaussian probability density function (PDF). This extends the scaling, MSD t1/2, seen in a constant density file. • When, in addition, the particles' diffusion coefficients are distributed like a power law with an exponent γ (around the origin), the MSD follows MSD t[1-γ]/[2/(1+a) - γ], with a Gaussian PDF. • In anomalous files that are renewal, namely, when all particles attempt a jump together, yet, with jump times taken from a PDF that decays as a power law with an exponent -1 - ɛ, ψ(t) t-1-ɛ, the MSD scales like the MSD of the corresponding normal file, in the power ɛ. • In anomalous files of independent particles, the MSD is very slow and scales like MSD log2(t). Even more exciting, the particles form clusters, defining a dynamical phase transition: depending on the anomaly power ɛ, the percentage of particles in clusters ξ follows ξ = √ {1-ǎrepsilon3}, yet when ɛ > 1, fluidity rather than clusters is seen. We talk about utilizing these results while focusing on biophysical processes and applications: dynamics in channels, membranes, biosensors, etc. Special Issue Comments: In this article, results about recently suggested variants in single file dynamics appear: heterogeneous files and slow files, yet also, the relevance with biophysical processes. It is related to the Special Issue articles about expansions in files,61 files with force,62 and the zig zag occurrences in files.63

  6. Oscillation characteristics of endodontic files: numerical model and its validation.

    Science.gov (United States)

    Verhaagen, Bram; Lea, Simon C; de Bruin, Gerrit J; van der Sluis, Luc W M; Walmsley, A Damien; Versluis, Michel

    2012-11-01

    During a root canal treatment, an antimicrobial fluid is injected into the root canal to eradicate all bacteria from the root canal system. Agitation of the fluid using an ultrasonically vibrating miniature file results in a significant improvement in the cleaning efficacy over conventional syringe irrigation. Numerical analysis of the oscillation characteristics of the file, modeled as a tapered, driven rod, shows a sinusoidal wave pattern with an increase in amplitude and decrease in wavelength toward the free end of the file. Measurements of the file oscillation with a scanning laser vibrometer show good agreement with the numerical simulation. The numerical model of endodontic file oscillation has the potential for predicting the oscillation pattern and fracture likeliness of various file types and the acoustic streaming they induce during passive ultrasonic irrigation.

  7. Media analyses based on Microsoft NTFS file ownership.

    Science.gov (United States)

    Kerr, Fred C

    2006-10-16

    The ever-increasing size of digital media presents a continuous challenge to digital investigators who must rapidly assess computer media to find and identify evidence. To meet this challenge, methods must continuously be sought to expedite the examination process. This paper investigates using the file ownership property as an analytical tool focusing on activity by individuals associated with the computer. Research centered on the New Technology File System (NTFS), which is the default file system in Microsoft Windows Operating System (OS). This was done because Microsoft's worldwide market penetration makes Windows and NTFS the most likely OS and file system to be encountered in digital forensic examinations. Significantly, digital forensic software now allows examination of NTFS file attributes and properties including the ownership property. The paper outlines potential limitations regarding interpreting ownership findings, and suggests areas for further research. Overall, file ownership is seen as a potentially viable new digital forensic tool.

  8. Sandia Data Archive (SDA) file specifications

    Energy Technology Data Exchange (ETDEWEB)

    Dolan, Daniel H. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Ao, Tommy [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-02-01

    The Sandia Data Archive (SDA) format is a specific implementation of the HDF5 (Hierarchal Data Format version 5) standard. The format was developed for storing data in a universally accessible manner. SDA files may contain one or more data records, each associated with a distinct text label. Primitive records provide basic data storage, while compound records support more elaborate grouping. External records allow text/binary files to be carried inside an archive and later recovered. This report documents version 1.0 of the SDA standard. The information provided here is sufficient for reading from and writing to an archive. Although the format was original designed for use in MATLAB, broader use is encouraged.

  9. INSPIRE and SPIRES Log File Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Adams, Cole; /Wheaton Coll. /SLAC

    2012-08-31

    SPIRES, an aging high-energy physics publication data base, is in the process of being replaced by INSPIRE. In order to ease the transition from SPIRES to INSPIRE it is important to understand user behavior and the drivers for adoption. The goal of this project was to address some questions in regards to the presumed two-thirds of the users still using SPIRES. These questions are answered through analysis of the log files from both websites. A series of scripts were developed to collect and interpret the data contained in the log files. The common search patterns and usage comparisons are made between INSPIRE and SPIRES, and a method for detecting user frustration is presented. The analysis reveals a more even split than originally thought as well as the expected trend of user transition to INSPIRE.

  10. Distributed Data Management and Distributed File Systems

    CERN Document Server

    Girone, Maria

    2015-01-01

    The LHC program has been successful in part due to the globally distributed computing resources used for collecting, serving, processing, and analyzing the large LHC datasets. The introduction of distributed computing early in the LHC program spawned the development of new technologies and techniques to synchronize information and data between physically separated computing centers. Two of the most challenges services are the distributed file systems and the distributed data management systems. In this paper I will discuss how we have evolved from local site services to more globally independent services in the areas of distributed file systems and data management and how these capabilities may continue to evolve into the future. I will address the design choices, the motivations, and the future evolution of the computing systems used for High Energy Physics.

  11. Distributed Data Management and Distributed File Systems

    Science.gov (United States)

    Girone, Maria

    2015-12-01

    The LHC program has been successful in part due to the globally distributed computing resources used for collecting, serving, processing, and analyzing the large LHC datasets. The introduction of distributed computing early in the LHC program spawned the development of new technologies and techniques to synchronize information and data between physically separated computing centers. Two of the most challenges services are the distributed file systems and the distributed data management systems. In this paper I will discuss how we have evolved from local site services to more globally independent services in the areas of distributed file systems and data management and how these capabilities may continue to evolve into the future. I will address the design choices, the motivations, and the future evolution of the computing systems used for High Energy Physics.

  12. A History of the Andrew File System

    CERN Multimedia

    CERN. Geneva; Altman, Jeffrey

    2011-01-01

    Derrick Brashear and Jeffrey Altman will present a technical history of the evolution of Andrew File System starting with the early days of the Andrew Project at Carnegie Mellon through the commercialization by Transarc Corporation and IBM and a decade of OpenAFS. The talk will be technical with a focus on the various decisions and implementation trade-offs that were made over the course of AFS versions 1 through 4, the development of the Distributed Computing Environment Distributed File System (DCE DFS), and the course of the OpenAFS development community. The speakers will also discuss the various AFS branches developed at the University of Michigan, Massachusetts Institute of Technology and Carnegie Mellon University.

  13. Electronic Document Management Using Inverted Files System

    Directory of Open Access Journals (Sweden)

    Suhartono Derwin

    2014-03-01

    Full Text Available The amount of documents increases so fast. Those documents exist not only in a paper based but also in an electronic based. It can be seen from the data sample taken by the SpringerLink publisher in 2010, which showed an increase in the number of digital document collections from 2003 to mid of 2010. Then, how to manage them well becomes an important need. This paper describes a new method in managing documents called as inverted files system. Related with the electronic based document, the inverted files system will closely used in term of its usage to document so that it can be searched over the Internet using the Search Engine. It can improve document search mechanism and document save mechanism.

  14. Electronic Document Management Using Inverted Files System

    Science.gov (United States)

    Suhartono, Derwin; Setiawan, Erwin; Irwanto, Djon

    2014-03-01

    The amount of documents increases so fast. Those documents exist not only in a paper based but also in an electronic based. It can be seen from the data sample taken by the SpringerLink publisher in 2010, which showed an increase in the number of digital document collections from 2003 to mid of 2010. Then, how to manage them well becomes an important need. This paper describes a new method in managing documents called as inverted files system. Related with the electronic based document, the inverted files system will closely used in term of its usage to document so that it can be searched over the Internet using the Search Engine. It can improve document search mechanism and document save mechanism.

  15. 78 FR 34370 - Revisions to Electric Quarterly Report Filing Process; Notice of Availability of Video Showing...

    Science.gov (United States)

    2013-06-07

    ... processes for filing EQRs allows an EQR seller and its agent to file using a web interface that generally replicates the Commission-distributed software used currently. A video showing how EQRs can be filed using...

  16. 12 CFR 11.3 - Filing requirements and inspection of documents.

    Science.gov (United States)

    2010-01-01

    ...) Electronic filings. An electronic filing of a statement required under section 16(a) of the 1934 Act that is..., whichever is currently in effect, shall be deemed filed on the same business day. (4) Mandatory compliance...

  17. Defining File Format Obsolescence: A Risky Journey

    OpenAIRE

    David Pearson; Colin Webb

    2008-01-01

    File format obsolescence is a major risk factor threatening the ongoing usefulness of digital information collections. While the preservation community has become increasingly interested in tools for assessing a wide range of risks, the National Library of Australia is developing mechanisms specifically focused on the risks of format obsolescence. The paper reports on the AONS II Project, undertaken in conjunction with the Australian Partnership for Sustainable Repositories (APSR). The projec...

  18. A distributed file service based on optimistic concurrency control

    OpenAIRE

    Mullender, Sape J.; Tanenbaum, Andrew S.

    1985-01-01

    The design of a layered file service for the Amoeba Distributed System is discussed, on top of which various applications can easily be intplemented. The bottom layer is formed by the Amoeba Block Services, responsible for implementing stable storage and repficated, highly available disk blocks. The next layer is formed by the Amoeba File Service which provides version management and concurrency control for tree-structured files. On top of this layer, the appficafions, ranging from databases ...

  19. 43 CFR 4.1181 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1181 Section 4.1181 Public...) Or 521(a)(3) Orders of Cessation § 4.1181 Who may file. (a) An application for review of an order of cessation may be filed under this section, whenever temporary relief has not been granted under section 525...

  20. 76 FR 62791 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-10-11

    ...: 20110928 TCC-Midway Farms Wind PDA to be effective 8/ 31/2011. Filed Date: 09/28/2011. Accession Number... filing per ] 35.13(a)(2)(iii: TNC-White Camp Solar PDA to be effective 9/19/2011. Filed Date: 09/28/2011..., October 19, 2011. Docket Numbers: ER11-4666-000. Applicants: NaturEner Glacier Wind Energy 1, LLC...

  1. Short-Term File Reference Patterns in a UNIX Environment,

    Science.gov (United States)

    1986-03-01

    files, this sharing is restricted to standard system files. We see very little sharing of user files. This paper emphasizes the implications the...daemon 166761 33.4% 13 0.1% 12830 library 59245 11.9% 222 1.4% 267 manual pages 18371 3.7% 1597 9.8% 11.5 news 40022 8,0% 5828 35.6% 6.9 program

  2. Strategy on review method for JENDL High Energy File

    Energy Technology Data Exchange (ETDEWEB)

    Yamano, Naoki [Sumitomo Atomic Energy Industries Ltd., Tokyo (Japan)

    1998-11-01

    Status on review method and problems for a High Energy File of Japanese Evaluated Nuclear Data Library (JENDL-HE File) has been described. Measurements on differential and integral data relevant to the review work for the JENDL-HE File have been examined from a viewpoint of data quality and applicability. In order to achieve the work effectively, strategy on development of standard review method has been discussed as well as necessity of tools to be used in the review scheme. (author)

  3. File and object replication in data grids

    CERN Document Server

    Stockinger, H E; Allcock, B; Foster, I; Holtman, K; Tierney, B L

    2001-01-01

    Data replication is a key issue in a data grid and can be managed in different ways and at different levels of granularity: for example, at the file level or the object level. In the high-energy physics community, data grids are being developed to support the distributed analysis of experimental data. We have produced a prototype data replication tool, the Grid Data Management Pilot (GDMP) that is in production use in one physics experiment, with middleware provided by the Globus toolkit used for authentication, data movement and other purposes. We present a new, enhanced GDMP architecture and prototype implementation that uses Globus data-grid tools for efficient file replication. We also explain how this architecture can address object replication issues in an object-oriented database management system. File transfer over wide-area networks requires specific performance tuning in order to gain optimal data transfer rates. We present performance results obtained with GridFTP, an enhanced version of FTP, and ...

  4. Semantic File Annotation and Retrieval on Mobile Devices

    Directory of Open Access Journals (Sweden)

    Sadaqat Jan

    2011-01-01

    Full Text Available The rapid development of mobile technologies has facilitated users to generate and store files on mobile devices such as mobile phones and PDAs. However, it has become a challenging issue for users to efficiently and effectively search for files of interest in a mobile environment involving a large number of mobile nodes. This paper presents SemFARM framework which facilitates users to publish, annotate and retrieve files which are geographically distributed in a mobile network enabled by Bluetooth. The SemFARM framework is built on semantic web technologies in support of file retrieval on low-end mobile devices. A generic ontology is developed which defines a number of keywords, their possible domains and properties. Based on semantic reasoning, similarity degrees are computed to match user queries with published file descriptions. The SemFARM prototype is implemented using the Java mobile platform (J2ME. The performance of SemFARM is evaluated from a number of aspects in comparison with traditional mobile file systems and enhanced alternatives. Experimental results are encouraging showing the effectiveness of SemFARM in file retrieval. We can conclude that the use of semantic web technologies have facilitated file retrieval in mobile computing environments maximizing user satisfaction in searching for files of interest.

  5. RRB / SSI Interface Checkwriting Integrated Computer Operation Extract File (CHICO)

    Data.gov (United States)

    Social Security Administration — This monthly file provides SSA with information about benefit payments made to railroad retirement beneficiaries. SSA uses this data to verify Supplemental Security...

  6. DIGITAL ONCOLOGY PATIENT RECORD - HETEROGENEOUS FILE BASED APPROACH

    Directory of Open Access Journals (Sweden)

    Nikolay Sapundzhiev

    2010-12-01

    Full Text Available Introduction: Oncology patients need extensive follow-up and meticulous documentation. The aim of this study was to introduce a simple, platform independent file based system for documentation of diagnostic and therapeutic procedures in oncology patients and test its function.Material and methods: A file-name based system of the type M1M2M3.F2 was introduced, where M1 is a unique identifier for the patient, M2 is the date of the clinical intervention/event, M3 is an identifier for the author of the medical record and F2 is the specific software generated file-name extension.Results: This system is in use at 5 institutions, where a total of 11 persons on 14 different workstations inputted 16591 entries (files for 2370. The merge process was tested on 2 operating systems - when copied together all files sort up as expected by patient, and for each patient in a chronological order, providing a digital cumulative patient record, which contains heterogeneous file formats.Conclusion: The file based approach for storing heterogeneous digital patient related information is an reliable system, which can handle open-source, proprietary, general and custom file formats and seems to be easily scalable. Further development of software for automatic checks of the integrity and searching and indexing of the files is expected to produce a more user-friendly environment

  7. High-Performance, Multi-Node File Copies and Checksums for Clustered File Systems

    Science.gov (United States)

    Kolano, Paul Z.; Ciotti, Robert B.

    2012-01-01

    Modern parallel file systems achieve high performance using a variety of techniques, such as striping files across multiple disks to increase aggregate I/O bandwidth and spreading disks across multiple servers to increase aggregate interconnect bandwidth. To achieve peak performance from such systems, it is typically necessary to utilize multiple concurrent readers/writers from multiple systems to overcome various singlesystem limitations, such as number of processors and network bandwidth. The standard cp and md5sum tools of GNU coreutils found on every modern Unix/Linux system, however, utilize a single execution thread on a single CPU core of a single system, and hence cannot take full advantage of the increased performance of clustered file systems. Mcp and msum are drop-in replacements for the standard cp and md5sum programs that utilize multiple types of parallelism and other optimizations to achieve maximum copy and checksum performance on clustered file systems. Multi-threading is used to ensure that nodes are kept as busy as possible. Read/write parallelism allows individual operations of a single copy to be overlapped using asynchronous I/O. Multinode cooperation allows different nodes to take part in the same copy/checksum. Split-file processing allows multiple threads to operate concurrently on the same file. Finally, hash trees allow inherently serial checksums to be performed in parallel. Mcp and msum provide significant performance improvements over standard cp and md5sum using multiple types of parallelism and other optimizations. The total speed-ups from all improvements are significant. Mcp improves cp performance over 27x, msum improves md5sum performance almost 19x, and the combination of mcp and msum improves verified copies via cp and md5sum by almost 22x. These improvements come in the form of drop-in replacements for cp and md5sum, so are easily used and are available for download as open source software at http://mutil.sourceforge.net.

  8. DATA Act File B Object Class and Program Activity - Social Security

    Data.gov (United States)

    Social Security Administration — The DATA Act Information Model Schema Reporting Submission Specification File B. File B includes the agency object class and program activity detail obligation and...

  9. Adding Data Management Services to Parallel File Systems

    Energy Technology Data Exchange (ETDEWEB)

    Brandt, Scott [Univ. of California, Santa Cruz, CA (United States)

    2015-03-04

    The objective of this project, called DAMASC for “Data Management in Scientific Computing”, is to coalesce data management with parallel file system management to present a declarative interface to scientists for managing, querying, and analyzing extremely large data sets efficiently and predictably. Managing extremely large data sets is a key challenge of exascale computing. The overhead, energy, and cost of moving massive volumes of data demand designs where computation is close to storage. In current architectures, compute/analysis clusters access data in a physically separate parallel file system and largely leave it scientist to reduce data movement. Over the past decades the high-end computing community has adopted middleware with multiple layers of abstractions and specialized file formats such as NetCDF-4 and HDF5. These abstractions provide a limited set of high-level data processing functions, but have inherent functionality and performance limitations: middleware that provides access to the highly structured contents of scientific data files stored in the (unstructured) file systems can only optimize to the extent that file system interfaces permit; the highly structured formats of these files often impedes native file system performance optimizations. We are developing Damasc, an enhanced high-performance file system with native rich data management services. Damasc will enable efficient queries and updates over files stored in their native byte-stream format while retaining the inherent performance of file system data storage via declarative queries and updates over views of underlying files. Damasc has four key benefits for the development of data-intensive scientific code: (1) applications can use important data-management services, such as declarative queries, views, and provenance tracking, that are currently available only within database systems; (2) the use of these services becomes easier, as they are provided within a familiar file

  10. An integrated approach to a teaching file linked to PACS.

    Science.gov (United States)

    Wilkinson, Luke E; Gledhill, Sam R

    2007-12-01

    To meet the educational needs of a medical imaging department with a strong teaching commitment, a teaching file that uses digital data supplied by the institutional picture archiving and communications system (PACS) was required. This teaching file had to be easily used by the end users, have a simple submission process, be able to support multiple users, be searchable on all data fields, and implementing the teaching file must not incur any additional software or hardware costs. The teaching file developed to address this problem takes advantage of the database structure and capabilities of several components included in the commercial PACS installed at the hospital. MS Access is used to seamlessly integrate with the digital imaging and communication in medicine (DICOM) database of a normal work station that is part of the PACS. This integration allows relevant patient and study demographics to be copied from images of interest and then to be stored in a separate database as the back-end of the digital teaching file. When images for a particular teaching file case need to be reviewed, they are automatically retrieved and displayed from the main PACS database using an open application programming interface (API) connection defined on the PACS web server. Utilizing this open API connection means the teaching file contains only the relevant demographic information of each teaching file case; no image data is stored locally. The open API connection allows access to imaging data usually not encountered in a teaching file, allowing more comprehensive imaging case files to be developed by the radiologist. Other advantages of this teaching file design are that it does not duplicate image data, it is small allowing simple ongoing backup, and it can be opened with multiple users accessing the database without compromising data access or integrity.

  11. Storing files in a parallel computing system based on user-specified parser function

    Science.gov (United States)

    Faibish, Sorin; Bent, John M; Tzelnic, Percy; Grider, Gary; Manzanares, Adam; Torres, Aaron

    2014-10-21

    Techniques are provided for storing files in a parallel computing system based on a user-specified parser function. A plurality of files generated by a distributed application in a parallel computing system are stored by obtaining a parser from the distributed application for processing the plurality of files prior to storage; and storing one or more of the plurality of files in one or more storage nodes of the parallel computing system based on the processing by the parser. The plurality of files comprise one or more of a plurality of complete files and a plurality of sub-files. The parser can optionally store only those files that satisfy one or more semantic requirements of the parser. The parser can also extract metadata from one or more of the files and the extracted metadata can be stored with one or more of the plurality of files and used for searching for files.

  12. 76 FR 12724 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-03-08

    ... Company submits tariff filing per 35.13(a)(1): 02--25--11 Attach O ITO--RC to be effective 4/ 26/2011... filing per 35.13(a)(2)(iii: Corrections to PG&E's Wholesale Distribution Tariff to be effective 4/28/2010...

  13. 28 CFR 32.2 - Computation of time; filing.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Computation of time; filing. 32.2 Section 32.2 Judicial Administration DEPARTMENT OF JUSTICE PUBLIC SAFETY OFFICERS' DEATH, DISABILITY, AND EDUCATIONAL ASSISTANCE BENEFIT CLAIMS General Provisions § 32.2 Computation of time; filing. (a) In computing...

  14. [Scanning electron microscope study of chemically disinfected endodontic files].

    Science.gov (United States)

    Navarro, G; Mateos, M; Navarro, J L; Canalda, C

    1991-01-01

    Forty stainless steel endodontic files were observed at scanning electron microscopy after being subjected to ten disinfection cycles of 10 minutes each one, immersed in different chemical disinfectants. Corrosion was not observed on the surface of the files in circumstances that this study was made.

  15. 77 FR 27766 - Jamar, John P.; Notice of Filing

    Science.gov (United States)

    2012-05-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Jamar, John P.; Notice of Filing Take notice that on May 3, 2012, John P. Jamar submitted for filing, an application for authority to hold interlocking positions, pursuant to...

  16. 76 FR 39867 - Russell, John G.; Notice of Filing

    Science.gov (United States)

    2011-07-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Russell, John G.; Notice of Filing Take notice that on June 29, 2011, John G. Russell submitted for filing, an application for authority to hold interlocking positions, pursuant to...

  17. 77 FR 37392 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-06-21

    ...: ER11-4525-002; ER11-4524-001. Applicants: Middletown Coke Company, LLC, Haverhill North Coke Company. Description: Notice of Change in Status of Middletown Coke Company, LLC, et al. Filed Date: 6/8/12. Accession... Numbers: ER12-1979-000. Applicants: PBF Power Marketing LLC. Description: Revised Change in Status Filing...

  18. Apical extrusion of debris using reciprocating files and rotary ...

    African Journals Online (AJOL)

    titanium systems. Procedure: Sixty ... and instrumentation with the single-file systems was significantly faster than in the multi-file systems (P < 0.05). The. WaveOne group ... chips, pulp tissue, which contain microorganisms, may be extruded into ...

  19. 26 CFR 40.6091-1 - Place for filing returns.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 16 2010-04-01 2010-04-01 true Place for filing returns. 40.6091-1 Section 40.6091-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) MISCELLANEOUS EXCISE TAXES EXCISE TAX PROCEDURAL REGULATIONS § 40.6091-1 Place for filing returns. (a) Quarterly returns. Except as provided in paragraph (b) of...

  20. 75 FR 59247 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-09-27

    ... 07, 2010. Docket Numbers: ER10-2669-000. Applicants: ANP Bellingham Energy Company, LLC. Description: ANP Bellingham Energy Company, LLC submits tariff filing per 35.12: Baseline MBR Tariff Filing of ANP.... Applicants: ANP Blackstone Energy Company, LLC. Description: ANP Blackstone Energy Company, LLC submits...

  1. 76 FR 28018 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-05-13

    ... Service Company of New Mexico. Description: Public Service Company of New Mexico submits tariff filing per..., May 26, 2011. Docket Numbers: ER11-3525-000. Applicants: Round Rock Energy LLC. Description: Round Rock Energy LLC submits tariff filing per 35.15: Notice of Cancellation of MBR Tariff for Round Rock...

  2. Image files - RPD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...ftp://ftp.biosciencedbc.jp/archive/rpd/LATEST/rpd_gel_image.zip File size: 38.5 MB Simple search URL - Data ... License Update History of This Database Site Policy | Contact Us Image files - RPD | LSDB Archive ...

  3. 21 CFR 1309.34 - Acceptance for filing; defective applications.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Acceptance for filing; defective applications. 1309.34 Section 1309.34 Food and Drugs DRUG ENFORCEMENT ADMINISTRATION, DEPARTMENT OF JUSTICE... case of minor defects as to completeness, the Administrator may accept the application for filing with...

  4. Tuning HDF5 subfiling performance on parallel file systems

    Energy Technology Data Exchange (ETDEWEB)

    Byna, Suren [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Chaarawi, Mohamad [Intel Corp. (United States); Koziol, Quincey [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Mainzer, John [The HDF Group (United States); Willmore, Frank [The HDF Group (United States)

    2017-05-12

    Subfiling is a technique used on parallel file systems to reduce locking and contention issues when multiple compute nodes interact with the same storage target node. Subfiling provides a compromise between the single shared file approach that instigates the lock contention problems on parallel file systems and having one file per process, which results in generating a massive and unmanageable number of files. In this paper, we evaluate and tune the performance of recently implemented subfiling feature in HDF5. In specific, we explain the implementation strategy of subfiling feature in HDF5, provide examples of using the feature, and evaluate and tune parallel I/O performance of this feature with parallel file systems of the Cray XC40 system at NERSC (Cori) that include a burst buffer storage and a Lustre disk-based storage. We also evaluate I/O performance on the Cray XC30 system, Edison, at NERSC. Our results show performance benefits of 1.2X to 6X performance advantage with subfiling compared to writing a single shared HDF5 file. We present our exploration of configurations, such as the number of subfiles and the number of Lustre storage targets to storing files, as optimization parameters to obtain superior I/O performance. Based on this exploration, we discuss recommendations for achieving good I/O performance as well as limitations with using the subfiling feature.

  5. 75 FR 8689 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-02-25

    ... Company submits supplements to its 12/31/09 filing of updated market power analysis for study period of 12... Creek Energy LLC; Grays Harbor Energy LLC; Forward Energy LLC; Grand Ridge Energy LLC; Willow Creek.... Description: Algonquin Companies submits notice of change regarding market-based rate authority. Filed Date...

  6. 77 FR 27041 - Combined Notice of Filings # 1

    Science.gov (United States)

    2012-05-08

    ... LLC, Grays Harbor Energy LLC, Grand Ridge Energy LLC, Willow Creek Energy LLC, Hardee Power Partners... Market-Based Rate Authority of Spring Canyon Energy LLC, et al. Filed Date: 4/27/12. Accession Number... Updated Market Power Analysis for the Southeast Region. Filed Date: 4/27/12. Accession Number: 20120427...

  7. 75 FR 76710 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2010-12-09

    ...: Public Service Company of New Mexico submits its Compliance update of its Market Based Rates Tariff, FERC... on Tuesday, December 14, 2010. Docket Numbers: ER10-1611-002. Applicants: Grays Harbor Energy LLC. Description: Grays Harbor Energy LLC. submits tariff filing per 35: Supplemental Category 1 Exemption Filing...

  8. 21 CFR 606.170 - Adverse reaction file.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Adverse reaction file. 606.170 Section 606.170... Adverse reaction file. (a) Records shall be maintained of any reports of complaints of adverse reactions... thorough investigation of each reported adverse reaction shall be made. A written report of the...

  9. 76 FR 34223 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-06-13

    ...: Self-Certification of Mojave Solar LLC as an Exempt Wholesale Generator. Filed Date: 06/03/2011... following exempt wholesale generator filings: Docket Numbers: EG11-90-000. Applicants: El Segundo Energy Center LLC. Description: Notice of Self Certification of Exempt Wholesale Generator Status El Segundo...

  10. 76 FR 53896 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-08-30

    ... following exempt wholesale generator filings: Docket Numbers: EG11-119-000. Applicants: Copper Mountain Solar 1, LLC. Description: Copper Mountain Solar 1, LLC Notice of Self- Certification of Exempt Wholesale Generator Status. Filed Date: 08/22/2011. Accession Number: 20110822-5079. Comment Date: 5 p.m...

  11. 76 FR 59676 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-09-27

    ... received the following exempt wholesale generator filings: Docket Numbers: EG11-127-000. Applicants: Invenergy Illinois Solar I LLC. Description: Notice of Self-Certification of Exempt Wholesale Generator Status of Invenergy Illinois Solar I LLC. Filed Date: 09/16/2011. Accession Number: 20110916-5077...

  12. 77 FR 58536 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-09-21

    .... Applicants: Catalina Solar, LLC. Description: Notice of Self-Certification of Exempt Wholesale Generator... generator filings: Docket Numbers: EG12-110-000. Applicants: Crawfordsville Energy, LLC. Description: Notice of Self-Certification as an Exempt Wholesale Generator of Crawfordsville Energy, LLC. Filed Date: 9...

  13. 78 FR 25070 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-04-29

    ... that the Commission received the following exempt wholesale generator filings: Docket Numbers: EG13-28...-29-000 Applicants: Imperial Valley Solar 1, LLC Description: Imperial Valley Solar 1, LLC Notice of Self- Certification of Exempt Wholesale Generator Status. Filed Date: 4/19/13 Accession Number...

  14. 78 FR 29127 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-05-17

    ... following exempt wholesale generator filings: Docket Numbers: EG13-33-000. Applicants: Solar Star California XIX, LLC. Description: Self-Certification of EWG Status of Solar Star California XIX, LLC. Filed Date...-000. Applicants: Solar Star California XX, LLC. Description: Self-Certification of EWG Status of Solar...

  15. 78 FR 29126 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-05-17

    ... exempt wholesale generator filings: Docket Numbers: EG13-32-000. Applicants: Arlington Valley Solar Energy II, LLC. Description: Notice of Self-Certification of Exempt Wholesale Generator Status. Filed.... Docket Numbers: ER13-1430-000. Applicants: Arlington Valley Solar Energy II, LLC. Description...

  16. 78 FR 58531 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-09-24

    ... Take notice that the Commission received the following exempt wholesale generator filings: Docket Numbers: EG13-57-000 Applicants: Genesis Solar, LLC Description: Notice of Self-Certification of Exempt Wholesale Generator Status of Genesis Solar, LLC. Filed Date: 9/16/13 Accession Number: 20130916-5078...

  17. 78 FR 59664 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-09-27

    ... exempt wholesale generator filings: Docket Numbers: EG13-58-000. Applicants: NRG Delta LLC. Description: Notice of NRG Delta LLC for Self-Certification of Exempt Wholesale Generator Status. Filed Date: 9/19/13.... Applicants: Genesis Solar, LLC. Description: Genesis Solar, LLC Petition for Waiver from Order Nos. 888, 889...

  18. 77 FR 37895 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-06-25

    ... following exempt wholesale generator filings: Docket Numbers: EG12-76-000. Applicants: NRG Solar Avra Valley, LLC. Description: Notice of Self Certification of Exempt Wholesale Generator Status NRG Solar Avra... Exempt Wholesale Generator Status of Spearville 3, LLC. Filed Date: 6/13/12. Accession Number: 20120613...

  19. 78 FR 59921 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-09-30

    ... Commission received the following exempt wholesale generator filings: Docket Numbers: EG13-59-000. Applicants: Mountain View Solar, LLC. Description: Notice of Self-Certification of Exempt Wholesale Generator Status of Mountain View Solar, LLC. Filed Date: 9/20/13. Accession Number: 20130920-5119. Comments Due: 5 p.m. e.t...

  20. 76 FR 40903 - Maine Public Service Company; Notice of Filing

    Science.gov (United States)

    2011-07-12

    ... Energy Regulatory Commission Maine Public Service Company; Notice of Filing Take notice that on June 22, 2011, Maine Public Service Company submitted a filing notifying the Commission of its relinquishment... available for review in the Commission's Public Reference Room in Washington, DC. There is an ``e...

  1. 75 FR 18195 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-04-09

    ... received the following electric rate filings: Docket Numbers: ER10-374-001. Applicants: Medicine Bow Power Partners, LLC. Description: Withdrawal of Request for Waiver of Medicine Bow Power Partners, LLC. Filed Date: 03/26/2010. Accession Number: 20100326-5082. Comment Date: 5 p.m. Eastern Time on Friday, April...

  2. 78 FR 74126 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-12-10

    .... and New England Power Pool Participants Committee submit Installed Capacity Requirement, Hydro Quebec.... Comments Due: 5 p.m. ET 12/24/13. Docket Numbers: ER14-512-000. Applicants: NRG Power Marketing LLC. Description: NRG Power Marketing LLC submits tariff filing per 35: ] Compliance Filing for MBR Tariff to be...

  3. 18 CFR 385.2002 - Caption of filings (Rule 2002).

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Caption of filings (Rule 2002). 385.2002 Section 385.2002 Conservation of Power and Water Resources FEDERAL ENERGY... filing must begin with a caption that sets forth: (a) The docket designation, if any; (b) The words...

  4. 77 FR 74652 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-12-17

    ... Energy II LLC. Description: Supplemental Filing of Amended Co-Tenancy, and Shared Facilities Agreement to... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice that the Commission received the...

  5. 77 FR 70427 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-11-26

    ... and North Sky River Energy, LLC Shared Facilities Agreement to be effective 11/16/2012. Filed Date: 11... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice that the Commission received the...

  6. 75 FR 65621 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-10-26

    ... Ridge Energy LLC submits tariff filing per 35.13(a)(2)(iii): Amended Assignment, Co-Tenancy, and Shared... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 October 18, 2010. Take notice that the...

  7. 7 CFR 58.24 - Record of filing time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Record of filing time. 58.24 Section 58.24 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Or Grading and Reinstatement of Regrading § 58.24 Record of filing time. A record showing the date...

  8. 7 CFR 52.9 - Record of filing time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Record of filing time. 52.9 Section 52.9 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Regulations Governing Inspection and Certification Inspection Service § 52.9 Record of filing time. A record...

  9. 50 CFR 260.17 - Record of filing time.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Record of filing time. 260.17 Section 260.17 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC... Fishery Products for Human Consumption Inspection Service § 260.17 Record of filing time. A record showing...

  10. 78 FR 16848 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-03-19

    ... following electric corporate filings: Docket Numbers: EC13-80-000. Applicants: Berry Petroleum Company. Description: Berry Petroleum Company's Section 203 FPA Application. Filed Date: 3/11/13. Accession Number...: Docket Numbers: QF13-325-000. Applicants: IPS Power Engineering. Description: Form 556--Notice of self...

  11. 75 FR 32937 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-06-10

    ... tariff filing per 35: CMS Gen MI Power LLC Power Sales & Reactive Supply & Voltage Control Service Tariff... Ravenswood, LLC. Description: TC Ravenswood, LLC submits Rate Schedule FERC No 2 and Minimum Fuel Oil Supply... per 35: UE Reactive Supply and Voltage Control to be effective 6/1/2010. Filed Date: 06/01/2010...

  12. 76 FR 51963 - Cobra Pipeline Ltd.; Notice of Baseline Filings

    Science.gov (United States)

    2011-08-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Cobra Pipeline Ltd.; Notice of Baseline Filings Take notice that on August 12, 2011, Cobra Pipeline Ltd. submitted a revised baseline filing of their Statement of Operating...

  13. 78 FR 76828 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-12-19

    ... Cancellation of Duke Energy Florida OATT Certificate of Concurrence to be effective 12/27/ 2013. Filed Date: 12... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 Take notice that the Commission received the...

  14. 77 FR 68759 - Combined Notice of Filings #2

    Science.gov (United States)

    2012-11-16

    .... Applicants: Goshen Phase II LLC. Description: Notification of Non-Material Change in Status of Goshen Phase...-Material Change in Status re: Minonk. Filed Date: 11/7/12. Accession Number: 20121107-5079. Comments Due: 5.... Description: Supplement to Notice of Change in Status of Canandaigua Power Partners LLC, et al. Filed Date: 10...

  15. 48 CFR 6104.402 - Filing claims [Rule 402].

    Science.gov (United States)

    2010-10-01

    ... SERVICES ADMINISTRATION TRAVEL AND RELOCATION EXPENSES CASES 6104.402 Filing claims . (a) Filing claims. A... shall be in writing and must be signed by the claimant or by the claimant's attorney or authorized...) Claim forwarded by agency on behalf of claimant. If an agency has denied a claim for travel or...

  16. 77 FR 74841 - Combined Notice of Filings #2

    Science.gov (United States)

    2012-12-18

    ... Transmission Rate Case Exhibit No. SPPC-9.2 Depreciation Study and Supporting Testimony Filed with the Public... Power Marketing LLC, Alcoa Power Generating Inc. Description: Notice of Non-Material Change in Status of Alcoa Power Marketing LLC, et al. Filed Date: 12/11/2012. Accession Number: 20121211-5100. Comments Due...

  17. 78 FR 77116 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-12-20

    ..., Inc. Description: Southwest Power Pool, Inc. submits tariff filing per 35.13(a)(2)(iii: KCPL and GMO... under Section 204 of the Federal Power Act and Part 34 of the Commission's Regulations. Filed Date: 12... 214 of the Commission's Regulations (18 CFR 385.211 and 385.214) on or before 5:00 p.m. Eastern time...

  18. 77 FR 59920 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-10-01

    ... the following electric rate filings: Docket Numbers: ER11-1933-002; ER10-2441-002. Applicants: Green Mountain Power Corporation, Central Vermont Public Service Corporation. Description: Green Mountain Power... Receivable Finance, LLC. Description: MBR Cancellation to be effective 11/20/2012. Filed Date: 9/21/12...

  19. 76 FR 48841 - Liberty Gas Storage, LLC; Notice of Filing

    Science.gov (United States)

    2011-08-09

    ... Energy Regulatory Commission Liberty Gas Storage, LLC; Notice of Filing Take notice that on July 25, 2011, Liberty Gas Storage, LLC (Liberty) submitted a request for confirmation that it is not required to file... Liberty exceed 200,000 Dth for three consecutive calendar years. Liberty argues that given the low levels...

  20. 40 CFR 1620.3 - Administrative claim; who may file.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Administrative claim; who may file... ADMINISTRATIVE CLAIMS ARISING UNDER THE FEDERAL TORT CLAIMS ACT § 1620.3 Administrative claim; who may file. (a) A claim for damage to or loss of property may be presented by the owner of the property, or his or...

  1. 43 CFR 4.1361 - Who may file.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Who may file. 4.1361 Section 4.1361 Public... Lands) and for Coal Exploration Permits (federal Program) § 4.1361 Who may file. The applicant, permittee, or any person having an interest which is or may be adversely affected by a decision of OSMRE set...

  2. 76 FR 69252 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-11-08

    ..., Victory Garden Phase IV, LLC, Waymart Wind Farm, L.P., Wessington Wind Energy Center, LLC, White Oak... following exempt wholesale generator filings: Docket Numbers: EG12-7-000. Applicants: Manzana Wind LLC. Description: Self-Certification of Exempt Wholesale Generator Status of Manzana Wind LLC. Filed Date: 10/31...

  3. 76 FR 5380 - Advisory Committees; Filing of Closed Meeting Reports

    Science.gov (United States)

    2011-01-31

    ... HUMAN SERVICES Food and Drug Administration Advisory Committees; Filing of Closed Meeting Reports AGENCY... Congress the annual reports of those FDA advisory committees that held closed meetings during fiscal year....C. app.1) and 21 CFR 14.60(d), FDA has filed with the Library of Congress the annual reports for the...

  4. 78 FR 64883 - Filing Financial and Other Reports

    Science.gov (United States)

    2013-10-30

    ... ADMINISTRATION 12 CFR Parts 741 and 748 RIN 3313-AE25 Filing Financial and Other Reports AGENCY: National Credit... to amend its ] regulations regarding filing financial, statistical, and other reports and credit... information as required by the Board.\\1\\ The Act directs each FICU to make reports of condition to the Board...

  5. 76 FR 22688 - Combined Notice of Filings #2

    Science.gov (United States)

    2011-04-22

    ... generator filings: Docket Numbers: EG11-74-000. Applicants: Yuba City Cogeneration Partners, LP. Description: Self-Certification of Yuba City Cogeneration Partners, LP. Filed Date: 04/15/2011. Accession Number.... Applicants: Yuba City Cogeneration Partners, LP. Description: Yuba City Cogeneration Partners, LP submits...

  6. 76 FR 48806 - Endangered Species; File No. 16194

    Science.gov (United States)

    2011-08-09

    ... for Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 16194... appointment in the following offices: Permits, Conservation and Education Division, Office of Protected..., Permits, Conservation and Education Division. by e-mail to [email protected] (include the File No...

  7. 75 FR 67682 - Endangered Species; File No. 15566

    Science.gov (United States)

    2010-11-03

    ... Protected Species (APPS) home page, https://apps.nmfs.noaa.gov , and then selecting File No. 15566 from the... appointment in the following offices: Permits, Conservation and Education Division, Office of Protected..., Permits, Conservation and Education Division: By e-mail to [email protected] (include the File No...

  8. 12 CFR Appendix C to Part 360 - Deposit File Structure

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Deposit File Structure C Appendix C to Part 360 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY RESOLUTION AND RECEIVERSHIP RULES Pt. 360, App. C Appendix C to Part 360—Deposit File Structure This is the...

  9. 75 FR 79367 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-12-20

    ... Numbers: ER11-2311-000. Applicants: Freedom Logistics, LLC. Description: Freedom Logistics, LLC submits tariff filing per 35.1: Freedom Logistics FERC Tariff to be effective 12/8/2010. Filed Date: 12/08/2010... p.m. Eastern Time on Wednesday, December 29, 2010. Docket Numbers: ER11-2314-000. Applicants: City...

  10. 29 CFR 8.8 - Filing of administrative record.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Filing of administrative record. 8.8 Section 8.8 Labor Office of the Secretary of Labor PRACTICE BEFORE THE ADMINISTRATIVE REVIEW BOARD WITH REGARD TO FEDERAL SERVICE CONTRACTS Review of Other Proceedings and Related Matters § 8.8 Filing of administrative record...

  11. 36 CFR 8.8 - Filing of labor agreements.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Filing of labor agreements. 8.8 Section 8.8 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR LABOR STANDARDS APPLICABLE TO EMPLOYEES OF NATIONAL PARK SERVICE CONCESSIONERS § 8.8 Filing of labor...

  12. 20 CFR 217.10 - Application filed after death.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Application filed after death. 217.10 Section... APPLICATION FOR ANNUITY OR LUMP SUM Applications § 217.10 Application filed after death. (a) A survivor... expenses dies before applying for the lump-sum death payment under part 234 of this chapter. The...

  13. 75 FR 141 - Big Rivers Electric Corporation; Notice of Filing

    Science.gov (United States)

    2010-01-04

    ...: E9-31089] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. NJ09-3-001] Big... Order and Granting Waivers,'' Big Rivers Elec. Corp., 128 FERC ] 61,264 (2009) (September 17 Order), Big... protests must be filed on or before the comment date. Anyone filing a motion to intervene or protest must...

  14. 76 FR 7837 - Big Rivers Electric Corporation; Notice of Filing

    Science.gov (United States)

    2011-02-11

    ... December 1, 2010, the date that Big Rivers integrated its transmission facilities with the Midwest... Energy Regulatory Commission Big Rivers Electric Corporation; Notice of Filing Take notice that on February 4, 2011, Big Rivers Electric Corporation (Big Rivers) filed a notice of cancellation of its Second...

  15. 76 FR 36104 - Combined Notice of Filings No. 2

    Science.gov (United States)

    2011-06-21

    ... the period April 2010 through March 2011 of Equitrans, L.P. Filed Date: 06/03/2011. Accession Number: 20110603-5330. Comment Date: 5 p.m. Eastern Time on Monday, June 20, 2011. Docket Numbers: RP11-2092-001. Applicants: Big Sandy Pipeline, LLC. Description: Big Sandy Pipeline, LLC submits tariff filing per 154.203...

  16. 77 FR 56831 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-09-14

    ... following exempt wholesale generator filings: Docket Numbers: EG12-106-000. Applicants: Limon Wind, LLC. Description: Notice of Self-Certification of Exempt Wholesale Generator Status of Limon Wind, LLC. Filed Date...-000. Applicants: Limon Wind II, LLC. Description: Notice of Self-Certification of Exempt Wholesale...

  17. 48 CFR 204.805 - Disposal of contract files.

    Science.gov (United States)

    2010-10-01

    ... eligible for destruction. If no space is available locally, transfer the files to the General Services... soon as practicable once they are no longer needed. (5) Retain pricing review files, containing documents related to reviews of the contractor's price proposals, subject to cost or pricing data (see FAR...

  18. 76 FR 21728 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2011-04-18

    ... Irrigation District Negotiated Rates to be effective 4/1/2011. Filed Date: 03/31/2011. Accession Number... on Tuesday, April 12, 2011. Docket Numbers: RP11-1952-000. Applicants: Transcontinental Gas Pipe Line Company, LLC. Description: Transcontinental Gas Pipe Line Company, LLC submits tariff filing per 154.204...

  19. 77 FR 5201 - Ecolab, Inc.; Filing of Food Additive Petition

    Science.gov (United States)

    2012-02-02

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 173 Ecolab, Inc.; Filing of Food Additive... Administration (FDA) is announcing that Ecolab, Inc., has filed a petition proposing that the food additive... Ecolab, Inc., 370 North Wabasha St., St. Paul, MN 55102-1390. The petition proposes to amend the food...

  20. 76 FR 18542 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-04-04

    ...-2997-001 Applicants: Vectren Retail, LLC Description: Vectren Retail, LLC submits tariff filing per 35: Vectren Retail d/b/a Vectren Source, Supplement to Initial MBR Application to be effective 4/29/2011...- recertifications. Any person seeking to challenge such qualifying facility status may do so by filing a motion...

  1. 37 CFR 2.111 - Filing petition for cancellation.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Filing petition for cancellation. 2.111 Section 2.111 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Cancellation § 2.111 Filing petition for...

  2. 37 CFR 2.59 - Filing substitute specimen(s).

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Filing substitute specimen(s). 2.59 Section 2.59 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Drawing § 2.59 Filing substitute specimen(s). (a...

  3. 37 CFR 2.101 - Filing an opposition.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Filing an opposition. 2.101 Section 2.101 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Opposition § 2.101 Filing an opposition. (a) An opposition...

  4. 37 CFR 2.34 - Bases for filing.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Bases for filing. 2.34 Section 2.34 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES The Written Application § 2.34 Bases for filing. (a) The...

  5. CONCEALMENT OF PRIVATE INFORMATION USING THE SPECIAL FILE SYSTEM

    Directory of Open Access Journals (Sweden)

    Alexander V. Remizov

    2013-01-01

    Full Text Available The article deals with methods of information hiding at the level of file system. Advantages and disadvantages of existing methods are given. The new method of information hiding with the use of specific file system is offered. Comparative results of existing works and offered method connected with capacity, speedwork and stability to detection are given.

  6. 20 CFR 655.1302 - Required pre-filing activity.

    Science.gov (United States)

    2010-04-01

    ... EMPLOYMENT OF FOREIGN WORKERS IN THE UNITED STATES Labor Certification Process for Temporary Agricultural Employment in the United States (H-2A Workers) § 655.1302 Required pre-filing activity. (a) Time of filing of... application for H-2A workers. If the job opportunity is located in more than one State, the employer may...

  7. 75 FR 18201 - Wisconsin Electric Power Company; Notice of Filing

    Science.gov (United States)

    2010-04-09

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Wisconsin Electric Power Company; Notice of Filing April 2, 2010. Take notice that on March 26, 2010, Wisconsin Electric Power Company filed counterpart signature pages to the...

  8. 76 FR 12098 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2011-03-04

    ... the following electric corporate filings: Docket Numbers: EC11-36-000 ] Applicants: BP Wind Energy..., 2011 Docket Numbers: ER11-2588-001 Applicants: Power Receivable Finance, LLC Description: Power Receivable Finance, LLC submits tariff filing per 35.17(b): PRF Substitute First Revised MBR Tariff to be...

  9. 40 CFR 716.25 - Adequate file search.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Adequate file search. 716.25 Section... ACT HEALTH AND SAFETY DATA REPORTING General Provisions § 716.25 Adequate file search. The scope of a person's responsibility to search records is limited to records in the location(s) where the required...

  10. 78 FR 59665 - Combined Notice of Filings #2

    Science.gov (United States)

    2013-09-27

    ... following electric corporate filings: Docket Numbers: EC13-152-000. Applicants: Capital Power Investments...: 20130920-5066. Comments Due: 5 p.m. ET 10/21/13. Docket Numbers: ER13-79-002. Applicants: Public Service Company of New Mexico. Description: Order 1000 IntraRegional Compliance Filing to be effective 12/31/9998...

  11. 76 FR 358 - Combined Notice of Filings # 1

    Science.gov (United States)

    2011-01-04

    ... Time on Tuesday, January 11, 2011. Docket Numbers: ER11-2456-000. Applicants: University of Pennsylvania. Description: University of Pennsylvania submits tariff filing per 35.1: Baseline Tariff Filing to... listed as a contact for an intervenor must create and validate an eRegistration account using the e...

  12. 5 CFR 293.403 - Contents of employee performance files.

    Science.gov (United States)

    2010-01-01

    ... records used to track employee performance during the appraisal period.) (6) Any form or other document... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Contents of employee performance files... REGULATIONS PERSONNEL RECORDS Employee Performance File System Records § 293.403 Contents of employee...

  13. 76 FR 43681 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-07-21

    ..., August 03, 2011. Docket Numbers: ER11-4041-000. Applicants: Verde Energy USA Trading, LLC. Description: Verde Energy USA Trading, LLC submits tariff filing per 35.12: Verde Energy MBR Application Filing to be... Marketing, LLC, Milford Wind Corridor Phase I, LLC, Milford Wind Corridor Phase II, LLC, Stetson Wind II...

  14. 75 FR 54615 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-09-08

    ... on Operation of Rate Schedule 7, Charges for Wind Forecasting Service. Filed Date: 08/16/2010... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 1 August 25, 2010. Take notice that the...

  15. 77 FR 65872 - Combined Notice of Filings #1

    Science.gov (United States)

    2012-10-31

    .... Description: TCC-Midway Farms Wind IA to be effective 9/24/2012. Filed Date: 10/23/12. Accession Number... Central Company. Description: TCC-Sendero Wind Energy PDA to be effective 10/16/ 2012. Filed Date: 10/23...

  16. 48 CFR 750.7110-5 - Contract files.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contract files. 750.7110-5 Section 750.7110-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT CONTRACT... Interests of the United States 750.7110-5 Contract files. The fully executed action memorandum indicating...

  17. 75 FR 76721 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2010-12-09

    ... Phase II Holdings LLC, Goshen Wind Holdings LLC. Description: Joint Application for Authorization of..., LLC. Description: Evergreen Wind Power III, LLC submits tariff filing per 35.12: MBR Application of... per 35.13(a)(2)(iii): Ameren-Dynegy DFA to be effective 11/1/2010. Filed Date: 11/30/2010. Accession...

  18. 21 CFR 720.3 - How and where to file.

    Science.gov (United States)

    2010-04-01

    ... VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.3 How and where to file. Forms FDA 2512 and FDA 2514 (“Discontinuance of Commercial Distribution of Cosmetic Product Formulation... or delivered to: Cosmetic Product Statement, Food and Drug Administration, 5100 Paint Branch Pkwy...

  19. 77 FR 33206 - Combined Notice of Filings #2

    Science.gov (United States)

    2012-06-05

    ..., Oasis Power Partners, LLC, Crescent Ridge LLC, Eurus Combine Hills II LLC, Avenal Park LLC, Sand Drag...: High Trail Wind Farm, LLC. Description: High Trail Wind Farm, LLC submits tariff filing per 35: High Trail Wind Farm First Revised MBR to be effective 5/26/2012. Filed Date: 5/25/12. Accession Number...

  20. 75 FR 68337 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-11-05

    ... Butte Wind Park, LLC. Description: Burley Butte Wind Park, LLC submits tariff filing per 35.12: Burley Butte Wind Park Market Based Rate Application to be effective 10/25/2010. Filed Date: 10/25/2010... Numbers: ER11-1882-000. Applicants: Golden Valley Wind Park, LLC. Description: Golden Valley Wind Park...

  1. 76 FR 49761 - Combined Notice of Filings #1

    Science.gov (United States)

    2011-08-11

    ... Energy Commercial Asset Management, Inc. Description: Notices of Cancellation of Duke Energy Commercial Asset Management, Inc. Filed Date: 08/05/2011. Accession Number: 20110805-5020. Comment Date: 5 p.m... to issue long-term debate. Filed Date: 08/02/2011. Accession Number: 20110805-0017. Comment Date: 5 p...

  2. 28 CFR 2.56 - Disclosure of Parole Commission file.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Disclosure of Parole Commission file. 2.56 Section 2.56 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS, YOUTH OFFENDERS, AND JUVENILE DELINQUENTS United States Code Prisoners and Parolees § 2.56 Disclosure of Parole Commission file....

  3. 78 FR 91 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-01-02

    .... Applicants: PacifiCorp. Description: Termination of BPA NITSA (Clark PUD) to be effective 12/7/2012. Filed....m. ET 1/11/13. Docket Numbers: ER13-611-000. Applicants: NorthWestern Corporation. Description: NorthWestern Corporation submits tariff filing per 35.13(a)(2)(iii: SA 663 664 665--Unexecuted...

  4. Integral test of JENDL fusion file

    Energy Technology Data Exchange (ETDEWEB)

    Maekawa, Fujio [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1997-03-01

    Integral test of JENDL Fusion File (J-FF) is performed through analyses of available benchmark experiments. As a result, good agreement between the calculated results with J-FF and the measured data is observed as a whole. Thus, J-FF is qualified to be used for nuclear design of fusion reactors. Owing to the high quality evaluation of J-FF, cross section data in J-FF for many nuclides are recommended to be assigned as data in FENDL/E-2.0 in the IAEA Consultants` Meeting held at Karlsruhe, Germany, 24-28 June, 1996. (author)

  5. Colour grading video files in Adobe Lightroom

    OpenAIRE

    Tommiska, Tarina

    2017-01-01

    The purpose of this thesis was to instruct the reader how to colour grade video files in a way that would be best suitable for photographers. This thesis suggests why video content should be colour graded in order to make an impact on the viewer and stand out in a meaningful way. I go through the very basics of colour theory to help the reader better understand the emotional impact of colour when observed. Colour theory sets the base for all colour grading and correction related work. ...

  6. 76 FR 20971 - Free Flow Power Corporation; Notice of Intent To File License Application, Filing of Pre...

    Science.gov (United States)

    2011-04-14

    ... Energy Regulatory Commission Free Flow Power Corporation; Notice of Intent To File License Application.... b. Project No. 13346-001. c. Dated Filed: February 18, 2011. d. Submitted By: Free Flow Power Corporation (Free Flow Power), on behalf of its subsidiary Paynebridge, LLC. e. Name of Project: Williams Dam...

  7. 75 FR 38805 - Filing Via the Internet; Electronic Tariff Filings Notice of Display of Time on Commission's...

    Science.gov (United States)

    2010-07-06

    ... [Federal Register Volume 75, Number 128 (Tuesday, July 6, 2010)] [Notices] [Page 38805] [FR Doc No: 2010-16284] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. RM07-16-000; RM01-5-000] Filing Via the Internet; Electronic Tariff Filings Notice of Display of Time on Commission's...

  8. 76 FR 8726 - Town of Edgartown; Notice of Intent To File License Application, Filing of Draft Application...

    Science.gov (United States)

    2011-02-15

    ...) 13 commercially operated OCGenTM horizontal hydrokinetic cross flow turbine generation units with a... Expedited Processing of a Hydrokinetic Pilot Project License Application, and Soliciting Comments a. Type of Filing: Notice of Intent to File a License Application for an Original License for a Hydrokinetic Pilot...

  9. 78 FR 35352 - Aviation Proceedings, Agreements Filed the Week Ending May 25, 2013

    Science.gov (United States)

    2013-06-12

    ... Office of the Secretary Aviation Proceedings, Agreements Filed the Week Ending May 25, 2013 The following... provisions. Answers may be filed within ] 21 days after the filing of the application. Docket Number: DOT-OST-2013-0110. Date Filed: May 24, 2013. Parties: Members of the International Air Transport Association...

  10. 78 FR 39435 - Aviation Proceedings, Agreements filed the week ending May 25, 2013

    Science.gov (United States)

    2013-07-01

    ... the week ending May 25, 2013 The following Agreements were filed with the Department of Transportation... procedures governing proceedings to enforce these provisions. Answers may be filed within 21 days after the filing of the application. Docket Number: DOT-OST-2012-0121. Date Filed: May 22, 2013. Parties: Members...

  11. 78 FR 33466 - Aviation Proceedings, Agreements Filed the Week Ending May 11, 2013

    Science.gov (United States)

    2013-06-04

    ... Office of the Secretary Aviation Proceedings, Agreements Filed the Week Ending May 11, 2013 The following... provisions. Answers may be filed within 21 days after the filing of the application. Docket Number: DOT-OST-2013-0098. Date Filed: May 8, 2013. Parties: Members of the International Air Transport Association...

  12. 77 FR 31421 - Aviation Proceedings, Agreements Filed the Week Ending May 12, 2012

    Science.gov (United States)

    2012-05-25

    ... Office of the Secretary Aviation Proceedings, Agreements Filed the Week Ending May 12, 2012 The following... provisions. Answers may be filed within 21 days after the filing of the application. Docket Number: DOT-OST-2012-0070 Date Filed: May 9, 2012 Parties: Members of the International Air Transport Association...

  13. 76 FR 33400 - Aviation Proceedings, Agreements Filed the Week Ending May 7, 2011

    Science.gov (United States)

    2011-06-08

    ... Office of the Secretary Aviation Proceedings, Agreements Filed the Week Ending May 7, 2011 The following... provisions. Answers may be filed within 21 days after the filing of the application. Docket Number: DOT-OST-2011-0090. Date Filed: May 5, 2011. Parties: Members of the International Air Transport Association...

  14. 15 CFR Appendix D to Part 30 - AES Filing Citation, Exemption and Exclusion Legends

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false AES Filing Citation, Exemption and... Appendix D to Part 30—AES Filing Citation, Exemption and Exclusion Legends I. USML Proof of Filing Citation AES ITN Example: AES X20060101987654. II. AES Proof of Filing Citation subpart A § 30.7 AES ITN...

  15. The Secret Prover : Proving Possession of Arbitrary Files While not Giving Them Away

    NARCIS (Netherlands)

    Teepe, Wouter

    2005-01-01

    The Secret Prover is a Java application which allows a user (A) to prove to another user (B), that A possesses a file. If B also possesses this file B will get convinced, and if B does not possess this file B will gain no information on (the contents of) this file. This is the first implementation

  16. 41 CFR 102-118.555 - Can a TSP file a supplemental administrative claim?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Can a TSP file a... 118-TRANSPORTATION PAYMENT AND AUDIT Claims and Appeal Procedures Transportation Service Provider (tsp) Filing Requirements § 102-118.555 Can a TSP file a supplemental administrative claim? Yes, a TSP may file...

  17. On-Board File Management and Its Application in Flight Operations

    Science.gov (United States)

    Kuo, N.

    1998-01-01

    In this paper, the author presents the minimum functions required for an on-board file management system. We explore file manipulation processes and demonstrate how the file transfer along with the file management system will be utilized to support flight operations and data delivery.

  18. ITER Creation Safety File Expertise Results

    Science.gov (United States)

    Perrault, D.

    2013-06-01

    In March 2010, the ITER operator delivered the facility safety file to the French "Autorité de Sûreté Nucléaire" (ASN) as part of its request for the creation decree, legally necessary before building works can begin on the site. The French "Institut de Radioprotection et de Sûreté Nucléaire" (IRSN), in support to the ASN, recently completed its expertise of the safety measures proposed for ITER, on the basis of this file and of additional technical documents from the operator. This paper presents the IRSN's main conclusions. In particular, they focus on the radioactive materials involved, the safety and radiation protection demonstration (suitability of risk management measures…), foreseeable accidents, building and safety important component design and, finally, wastes and effluents to be produced. This assessment was just the first legally-required step in on-going safety monitoring of the ITER project, which will include other complete regulatory re-evaluations.

  19. Literature-filing system for vulvar conditions.

    Science.gov (United States)

    Haefner, H K; Kamlapurker, M

    1997-08-01

    To develop a classification system for the retrieval of stored material on various vulvar conditions. The major subject areas of vulvar conditions and management were identified. They were further classified into categories and subcategories. A numbering system was chosen for coding. The information was formatted to allow retrieval from the Internet in a framework ready for label printing. The classification system developed provides guidelines that are useful in making retrieval of stored material on various vulvar conditions more efficient. It can be expanded and personalized to fit the practitioner's needs and can be obtained from the Internet in the following manner by opening Netscape and entering the location: http:/(/)www.med.umich.edu/obgyn/vulva/ vulvalit.html. The filing system was formatted to allow standard, 5266, file-folder-sized label sheets to be inserted into the printer, allowing access to pretyped labels. A total of 464 labels will be printed. This system for retrieving vulvar information will be useful to many people, including clinicians, educators and researchers. The information is easily obtained from the Internet and printed in label form. It aids in the development of a system for life-long learning.

  20. Dynamic Non-Hierarchical File Systems for Exascale Storage

    Energy Technology Data Exchange (ETDEWEB)

    Long, Darrell E. [Univ. of California, Santa Cruz, CA (United States); Miller, Ethan L [Univ. of California, Santa Cruz, CA (United States)

    2015-02-24

    This constitutes the final report for “Dynamic Non-Hierarchical File Systems for Exascale Storage”. The ultimate goal of this project was to improve data management in scientific computing and high-end computing (HEC) applications, and to achieve this goal we proposed: to develop the first, HEC-targeted, file system featuring rich metadata and provenance collection, extreme scalability, and future storage hardware integration as core design goals, and to evaluate and develop a flexible non-hierarchical file system interface suitable for providing more powerful and intuitive data management interfaces to HEC and scientific computing users. Data management is swiftly becoming a serious problem in the scientific community – while copious amounts of data are good for obtaining results, finding the right data is often daunting and sometimes impossible. Scientists participating in a Department of Energy workshop noted that most of their time was spent “...finding, processing, organizing, and moving data and it’s going to get much worse”. Scientists should not be forced to become data mining experts in order to retrieve the data they want, nor should they be expected to remember the naming convention they used several years ago for a set of experiments they now wish to revisit. Ideally, locating the data you need would be as easy as browsing the web. Unfortunately, existing data management approaches are usually based on hierarchical naming, a 40 year-old technology designed to manage thousands of files, not exabytes of data. Today’s systems do not take advantage of the rich array of metadata that current high-end computing (HEC) file systems can gather, including content-based metadata and provenance1 information. As a result, current metadata search approaches are typically ad hoc and often work by providing a parallel management system to the “main” file system, as is done in Linux (the locate utility), personal computers, and enterprise search

  1. Efficient load rebalancing for distributed file system in Clouds

    Directory of Open Access Journals (Sweden)

    Mr. Mohan S. Deshmukh

    2016-05-01

    Full Text Available Cloud computing is an upcoming era in software industry. It’s a very vast and developing technology. Distributed file systems play an important role in cloud computing applications based on map reduce techniques. While making use of distributed file systems for cloud computing, nodes serves computing and storage functions at the same time. Given file is divided into small parts to use map reduce algorithms in parallel. But the problem lies here since in cloud computing nodes may be added, deleted or modified any time and also operations on files may be done dynamically. This causes the unequal load distribution of load among the nodes which leads to load imbalance problem in distributed file system. Newly developed distributed file system mostly depends upon central node for load distribution but this method is not helpful in large-scale and where chances of failure are more. Use of central node for load distribution creates a problem of single point dependency and chances of performance of bottleneck are more. As well as issues like movement cost and network traffic caused due to migration of nodes and file chunks need to be resolved. So we are proposing algorithm which will overcome all these problems and helps to achieve uniform load distribution efficiently. To verify the feasibility and efficiency of our algorithm we will be using simulation setup and compare our algorithm with existing techniques for the factors like load imbalance factor, movement cost and network traffic.

  2. Cyclic Fatigue of ProFile Vortex and Vortex Blue Nickel-Titanium Files in Single and Double Curvatures.

    Science.gov (United States)

    Duke, Frederic; Shen, Ya; Zhou, Huimin; Ruse, N Dorin; Wang, Zhe-jun; Hieawy, Ahmed; Haapasalo, Markus

    2015-10-01

    The aims of this study were to determine the flexibility of ProFile Vortex (VX) and Vortex Blue (VB) files (Dentsply Tulsa Dental Specialties, Tulsa, OK) and then to evaluate and compare their fatigue resistance in artificial single curvature and 2 different artificial double curvature canals. Flexibility of the files (size 25/.04) in bending was assessed according to ISO 3630-1. Both files were subjected to fatigue tests inside artificial canals with a single curvature (group 1: 60° curvature, 5-mm radius) and with 2 different double curvatures (group 2: first [coronal] curve of 60° curvature and 5-mm radius and the second one [apical] of 30° curvature and 2-mm radius and group 3: first curve of 60° curvature and 5-mm radius and the second one of 60° curvature and 2-mm radius). The number of cycles to fracture (NCF) was recorded, and the fracture surface of all fragments was examined with a scanning electron microscope. The bending load was significantly lower for VB files than VX files (P < .05), and the 2 types of files followed different trajectories in identical canals. In group 1, the 2 files had significantly higher NCF than in groups 2 and 3 (P < .05). Both files had significantly higher NCF in group 2 than in group 3 (P < .05). In group 1, VB files had fatigue resistance superior to VX files (P < .05), whereas in groups 2 and 3 their fatigue resistance was not statistically different from each other. The crack initiation of a vast majority of files that fractured in double curvature canals (groups 2 and 3) was localized on either 1 of 2 of the 3 cutting edges. Double curvature canals represent a much more stressful and challenging anatomy than single curvature canals, and, in them, fatigue resistance may be affected by the degrees and the radii of curvatures as well as by the bending properties of the files. Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  3. Dental burs and endodontic files: are routine sterilization procedures effective?

    Science.gov (United States)

    Morrison, Archie; Conrod, Susan

    2009-02-01

    The complex miniature architecture of dental burs and endodontic files makes precleaning and sterilization difficult. Devising a sterilization protocol for endodontic files and dental burs requires care, and some have suggested that these instruments be considered single-use devices. One purpose of this study was to determine the effectiveness of various sterilization techniques currently used in dentistry for the resterilization of dental burs and endodontic files. The second aim was to determine whether new dental burs and endodontic files, as supplied in packages from the manufacturer, are sterile. The sterility of new (unused) and used dental burs and endodontic files before and after various sterilization procedures was analyzed. New burs and files were tested immediately after removal from manufacturers" packaging, with or without prior sterilization. Burs and files that had been used in various dental offices were precleaned, packaged, resterilized and then tested for various pathogens. Each item was individually removed from the sterilization packaging, transferred by sterile technique into Todd-Hewitt broth, incubated at 37 degrees C for 72 hours and observed for bacterial growth. Sterilization procedures were 100% effective for unused burs and unused files but were less than 100% effective for all other test groups. Contamination rates following sterilization ranged from 15% for one group of used burs (p = 0.01) to 58% for one group of used files (p sterile and should therefore be sterilized before first use. The resterilization procedures tested here were not adequate, and more rigorous sterilization procedures are needed. If such procedures cannot be devised, these instruments should perhaps be considered single-use devices.

  4. A Reputation System with Anti-Pollution Mechanism in P2P File Sharing Systems

    OpenAIRE

    Mei, Qi; Yajun, Guo; Huifang, Yan

    2009-01-01

    File pollution has become a very serious problem in peer-to-peer file sharing systems, because of which it greatly reduces the effectiveness of systems. Users downloaded pollution files not only consumed bandwidth, but were also likely to share polluted files without checking. If these polluted files carry a virus, Trojan horse, or other malicious code, the loss of users would be disastrous. There is much research done on reputation-based anti-pollution mechanisms. Peer reputation systems and...

  5. FileMaker Pro 11 The Missing Manual

    CERN Document Server

    Prosser, Susan

    2010-01-01

    This hands-on, friendly guide shows you how to harness FileMaker's power to make your information work for you. With a few mouse clicks, the FileMaker Pro 11 database helps you create and print corporate reports, manage a mailing list, or run your entire business. FileMaker Pro 11: The Missing Manual helps you get started, build your database, and produce results, whether you're running a business, pursuing a hobby, or planning your retirement. It's a thorough, accessible guide for new, non-technical users, as well as those with more experience. Start up: Get your first database up and runnin

  6. Parallel file system analysis through application I/O tracing

    OpenAIRE

    Wright, Steven A.; Hammond, Simon D.; Pennycook, Simon J.; Bird, Robert F.; Herdman, J. A.; Miller, I.; Vadgama, A.; Bhalerao, Abhir; Jarvis, Stephen A.

    2013-01-01

    Input/Output (I/O) operations can represent a significant proportion of the run-time of parallel scientific computing applications. Although there have been several advances in file format libraries, file system design and I/O hardware, a growing divergence exists between the performance of parallel file systems and the compute clusters that they support. In this paper, we document the design and application of the RIOT I/O toolkit (RIOT) being developed at the University of Warwick with our ...

  7. CIF (Crystallographic Information File): A Standard for Crystallographic Data Interchange

    Science.gov (United States)

    Brown, I. D.

    1996-01-01

    The Crystallographic Information File (CIF) uses the self-defining STAR file structure. This requires the creation of a dictionary of data names and definitions. A basic dictionary of terms needed to describe the crystal structures of small molecules was approved in 1991 and is currently used for the submission of papers to Acta Crystallographica C. A number of extensions to this dictionary are in preparation. By storing the dictionary itself as a STAR file, the definitions and relationships in the CIF dictionary become computer interpretable. This offers many possibilities for the automatic handling of crystallographic information. PMID:27805170

  8. Log files as a tool for improving Internet dictionaries

    DEFF Research Database (Denmark)

    Henning, Bergenholtz.; Johnsen, Mia

    2005-01-01

    lexicographers to monitor user behaviour in a different and much more precise way. Analyses of log files reveal exactly which lemmas and which types of information have been requested, and, perhaps more significantly, which lemmas and which types of information have been requested but were not found...... in the dictionary. Furthermore, log files allow lexicographers to see the types of information which have not, or not yet, been searched for. All in all, log files may thus be used as a tool for improving internet dictionaries - and perhaps also printed dictionaries - quite considerably....

  9. ESUSA: US endangered species distribution file

    Energy Technology Data Exchange (ETDEWEB)

    Nagy, J.; Calef, C.E.

    1979-10-01

    This report describes a file containing distribution data on endangered species of the United States of Federal concern pursuant to the Endangered Species Act of 1973. Included for each species are (a) the common name, (b) the scientific name, (c) the family, (d) the group (mammal, bird, etc.), (e) Fish and Wildlife Service (FWS) listing and recovery priorities, (f) the Federal legal status, (g) the geographic distribution by counties or islands, (h) Federal Register citations and (i) the sources of the information on distribution of the species. Status types are endangered, threatened, proposed, formally under review, candidate, deleted, and rejected. Distribution is by Federal Information Processing Standard (FIPS) county code and is of four types: designated critical habitat, present range, potential range, and historic range.

  10. The persistence of object file representations.

    Science.gov (United States)

    Noles, Nicholaus S; Scholl, Brian J; Mitroff, Stephen R

    2005-02-01

    Coherent visual experience of dynamic scenes requires not only that the visual system segment scenes into component objects but that these object representations persist, so that an object can be identified as the same object from an earlier time. Object files (OFs) are visual representations thought to mediate such abilities: OFs lie between lower level sensory processing and higher level recognition, and they track salient objects over time and motion. OFs have traditionally been studied via object-specific preview benefits (OSPBs), in which discriminations of an object's features are speeded when an earlier preview of those features occurred on the same object, as opposed to on a different object, beyond general displaywide priming. Despite its popularity, many fundamental aspects of the OF framework remain unexplored. For example, although OFs are thought to be involved primarily in online visual processing, we do not know how long such representations persist; previous studies found OSPBs for up to 1500 msec but did not test for longer durations. We explored this issue using a modified object reviewing paradigm and found that robust OSPBs persist for more than five times longer than has previously been tested-for at least 8 sec, and possibly for much longer. Object files may be the "glue" that makes visual experience coherent not just in online moment-by-moment processing, but on the scale of seconds that characterizes our everyday perceptual experiences. These findings also bear on research in infant cognition, where OFs are thought to explain infants' abilities to track and enumerate small sets of objects over longer durations.

  11. 76 FR 55897 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2011-09-09

    ..., 2011. Docket Numbers: RP11-2516-000. Applicants: Black Marlin Pipeline Company. Description: Black Marlin Pipeline Company submits tariff filing per 154.402: Annual Charge Adjustment to be effective 10/1...

  12. 75 FR 61741 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2010-10-06

    ..., 2010. Docket Numbers: RP10-1306-000. Applicants: Black Marlin Pipeline Company. Description: Black Marlin Pipeline Company submits tariff filing per 154.203: Baseline Tariff Resubmittal to be effective 8...

  13. 76 FR 6610 - Combined Notice of Filings No. 1

    Science.gov (United States)

    2011-02-07

    ...-000. Applicants: Black Marlin Pipeline Company. Description: Petition for Extension of Temporary Exemptions from Tariff Provisions of Black Marlin Pipeline Company. Filed Date: 01/20/2011. Accession Number...

  14. HCUP State Emergency Department Databases (SEDD) - Restricted Access File

    Data.gov (United States)

    U.S. Department of Health & Human Services — The State Emergency Department Databases (SEDD) contain the universe of emergency department visits in participating States. Restricted access data files are...

  15. Archive of Census Related Products (ACRP): 1992 Boundary Files

    Data.gov (United States)

    National Aeronautics and Space Administration — The 1992 Boundary Files portion of the Archive of Census Related Products (ACRP) consists of 1992 boundary data from the U.S. Census Bureau's Topologically...

  16. Archive of Census Related Products (ACRP): 1990 Standard Extract Files

    Data.gov (United States)

    National Aeronautics and Space Administration — The 1990 Standard Extract Files portion of the Archive of Census Related Products (ACRP) contains population and housing data derived from the U.S. Census Bureau's...

  17. 78 FR 21926 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-04-12

    ...-007; ER10-2678-006; ER10-2638-007. Applicants: ANP Bellingham Energy Company, LLC, ANP Blackstone.... Description: Notice of Non-Material Change in Status of ANP Bellingham Energy Company, LLC, et al. Filed Date...

  18. 22 CFR 41.103 - Filing an application.

    Science.gov (United States)

    2010-04-01

    ... custody of, or a legitimate interest in, the alien. (3) Waiver of filing of application when personal.... All documents required by the consular officer under the authority of § 41.105(a) are considered...

  19. 78 FR 35016 - Combined Notice of Filings #1

    Science.gov (United States)

    2013-06-11

    ... following electric rate filings: Docket Numbers: ER12-2159-003. Applicants: Canadian Hills Wind, LLC... Public Service Company. Description: Refund Report for MPP Westwing Substation Interconnection... Energy, Inc. Description: Westar Energy, Inc. submits Nemaha-Marshall Electric Cooperative Association...

  20. 75 FR 12535 - Combined Notice of Filings #1

    Science.gov (United States)

    2010-03-16

    ...-659-012; ER04- 657-012; ER04-660-012. Applicants: Astoria Generating Company, LP, Boston Generating...: California Independent System Operator Corporation submits errata to 3/1/2010 ISO's response. Filed Date: 03...