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Sample records for san pei fang

  1. Florence Fang Looks East

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    FLORENCE Fang, the Chinese immigrant who moved to San Francisco’s Chinatown as a young woman with little money and a great ambition, has become one of the most influential Asian American women in the United States. Fang is now chairwoman of Pan Asia Venture Capital Corporation, which oversees the activities of more than 20 companies including a handful of news publications, printing houses, restaurants, hotels, real estate and chemical industry.

  2. [Fang Chengpei and Zheng's laryngology].

    Science.gov (United States)

    Zheng, R

    1994-01-01

    The relationship between Fang Chengpei, a famous physician of the Xin'an school of traditional Chinese medicine and a master of traditional Chinese folk arts of the Qing Dynasty, and successors of Zheng's laryngology of Xin'an was even closer than that between his close relatives and friends. Zheng Meijian imparted laryngological medical skills to Fang, made a preface to Shou Yi Mi Lu (Secret Records of Medical Skills)--a secret document handed down in Fang-family, gave directions to and held discussions with Fang on the doctrine on five elements' motion and six kinds of natural factors. Fang participated in naming, prefacing, and revising Chong Lou Yu Yue (A Jade Key to Laryngeal Diseases) in which three secret recipes for laryngeal diseases that had been handed down in Fang's family were included. Together with Zheng Shufu, he wrote Chong Lou Yu Yue Xu Pian (Continuation of A Jade Key to Laryngeal Diseases). After Fang had passed away, many of his belongings were left in Zhengs' house, including medical documents, calligraphy, music scores, medical instruments, etc. Fang made a great contribution to the academic development of Zheng's Laryngology.

  3. XIAO Pei-gen

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Academician of Chinese Academy of Engineering Editor-in-chief of Chinese Herbal Medicines(CHM)Honorary director of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing100193,China Tel/Fax:+86-10-62894462 E-mail:xiaopg@public.bta.net.cn Professor XIAO Pei-gen is the founder of the Institute of Medicinal Plant Development(IMPLAD),Chinese Academy of Medical Sciences(CAMS).He is also one of the founders and leading

  4. Line-primitive Linear Spaces with Fang-Li Parameter gcd(k, r) at Most 12

    Institute of Scientific and Technical Information of China (English)

    Sheng Lin ZHOU; Yan Bo MA; Wei Dong FANG

    2011-01-01

    Let G be a subgroup of the automorphism group of a non-trivial finite linear space S. In this paper we prove that if G is line-primitive with Fang-Li parameter gcd(k, r) = 11 and 12, then G is also point-primitive. This extends the results of Fang and Li (1993) and Li and Liu (2001) which combined gives the result for gcd(k, r) ≤ 10.

  5. [The compilation, contents and spread of Qing li Shan jiu Fang (Formulary for Effective Rescuing in the Qingli Reign].

    Science.gov (United States)

    Han, Yi

    2015-01-01

    Qing li shan jiu fang (Formulary for Effective Rescuing in the Qingli Reign), compiled by Hanlin Academy of Medical Official in 1048 under the decree of the Emperor, is a medical formulary exclusively used to prevent and control poisonous parasite disease. It is composed of formulae provided by a medical scholar of Fuzhou, Lin Shiyuan, together with other formulae collected by the imperial physicians and so on. Unfortunately, it was lost about after the demise of the Southern Song Dynasty. However, in the Southern Song Dynasty, two books, Liang Kejia's Chun xi san shan zhi and Hong Mai's Yi jian zhi bu, do record the progress of its compilation, parts of its contents and the condition of its spread and application. Moreover, they also describe the kinds, the feature, the epidemic, prevention and cure of parasite poison. It is especially good that this book preserves three famous formulae, including Zhi gu du zheng fang (Orthodox Formula for Treating Parasitic Poisons) (called A Cathartic Formula with 8 Ingredients in the Yuan Dynasty), Jie du wan (Antidote Pills) and He qi tang san (Powder of Decoction for Harmonious Qi), which are of medical significance for the understanding of the property of Qing li shan jiu fang. The Song emperors, the central government and local officials all paid high attention to the spread and application of this book. They not only enacted it to the counties, and provinces, but also carved it on stone steles for popularizing the knowledge of preventing parasitic poisons to medical workers and common people.

  6. [Textual research on Newly Carved sun Zhenren's Qian jin fang].

    Science.gov (United States)

    Su, L

    1995-01-01

    As one of the ancient edition of Qian jin fang, this Newly Carved Sun Zhenren's Qian Jin Fang is far more authentic in the structure of its contents and its text, as compared to those editions of the Song Dynasty, and is, of course, closer to its original form and more reliable with textual significance. This article explores the distribution, characters of the edition and time of carving.

  7. Line-transitive point-imprimitive linear spaces with Fang-Li parameter gcd(k,r) at most 10

    CERN Document Server

    Guan, Haiyan; Zhou, Shenglin

    2011-01-01

    This paper is a further contribution to the classification of line-transitive finite linear spaces. We prove that if S is a non-trivial finite linear space with the Fang-Li parameter gcd(k,r) is 9 or 10, the automorphism group G of S is line-transitive and point-imprimitive, then S is the Desarguesian projective plane PG(2,9).

  8. Liu Fang Yuan at The Huntington——An Overview

    Institute of Scientific and Technical Information of China (English)

    (USA)Laurie Sowd

    2009-01-01

    Liu Fang Yuan is a classical Chinese Garden, the Garden of Flowing Fragrance, a most remarkable project in Huntington. This project was conceived by James Folsom. It is an international business venture, complex construction project, unique China-US collaboration, and center of cultural memory. Inspired by the centuries-old Chinese tradition of private gardens, Liu Fang Yuan combines the scenic beauty of nature with the expressiveness of literature to give deeper meaning to the landscape. True to the authentic nature of a Chinese garden, the design respects the site. It extends to adapting some of the traditional elements of Chinese garden design to meet local needs for seismic safety and wheelchair accessibility. Liu Fang Yuan is the only truly seasonal garden at the Huntington, emphasizing and symbolizing the progression of winter, spring, summer, and autumn. The visitor experiences more than the visual realm. The garden's name, Liu Fang Yuan, has bolh literal and symbolic meanings refer to the scent of flowers and trees, also echoes the name of famed Ming dynasty painter Li Liufang. Liu Fang Yuan is truly a microcosm of The Huntington, bringing together botany, art and literature in a garden that will serve as a place of cultural memory and center for education about one of the great humanities traditions of the world.

  9. The Effect of Linear PEI on Characteristics and Transfection Efficiency of PEI-Based Cationic Nanoliposomes

    Directory of Open Access Journals (Sweden)

    Mohammad Ramezani

    2011-01-01

    Full Text Available Objective(sThe development of efficient and safe carrier system to transfer DNA into cells is essential in non-viral gene therapy. The aim of the present study was to evaluate the effect of linear polyetheneimine (lPEI (2500 Da on the physicochemical and biological properties of lipopolyplexes constructed from liposomes and lPEI. Materials and MethodsDifferent lipopolymers were synthesized from lPEI and acrylate derivatives. Nanocarriers were composed of the lipids (DOPE, DPPE and DOTAP and the synthesized lipopolymers. After characterization of the prepared vectors by determination of size and zeta potential, transfection activity was tested in Neuro2A cells. Ethidium bromide and MTT test were used to evaluate the DNA condensation ability and cytotoxicity of vectors, respectively. Results Vector’s size ranged from 95 to 337 nm and they had positive charge. The differences in DNA binding properties of lipopolyplexes were not significant. Among lipids, DOTAP showed better impact on transfection efficiency. The highest transfection activity was achieved by liposomal formulation consist of DOTAP and lipopolymer composed of lPEI and hexyl acrylate. The lipopolyplexes showed minimum cytotoxicity to the cultured cells in vitro. Conclusion The results of study confirmed that it is possible to improve gene expression using lipopolyplexes.

  10. Minxin Pei, China's Crony Capitalism: The Dynamics of Regime Decay

    DEFF Research Database (Denmark)

    Brødsgaard, Kjeld Erik

    2017-01-01

    Book review of: Minxin Pei, China's Crony Capitalism: The Dynamics of Regime Decay. Cambridge, Mass.: Harvard University Press, 2016. 365 pp., including references and index. ISBN 9780674737297.......Book review of: Minxin Pei, China's Crony Capitalism: The Dynamics of Regime Decay. Cambridge, Mass.: Harvard University Press, 2016. 365 pp., including references and index. ISBN 9780674737297....

  11. IEOH MING PEI: UNA IDEA, TRES VARIACIONES

    Directory of Open Access Journals (Sweden)

    Alberto Donaire Rodríguez

    2010-05-01

    Full Text Available RESUMEN El texto siguiente es un resumen de mi lección de despedida, el 18 de noviembre de 2006, al jubilarme en la Escuela de Arquitectura de Sevilla. Como no se grabó y nunca llevo escritas mis clases, ésta es una reconstrucción basada en mi guión y en las notas tomadas por algunos asistentes, a los que agradezco su interés.Por vez primera se celebraba en la Escuela un acto de esta naturaleza, por iniciativa del entonces Director, Jaime Navarro Casas. A él agradezco su presencia y sus palabras, e igualmente a Juan Luis Trillo de Leyva, que hizo una especie de recorrido sentimental de mi paso por la Escuela, que escuché con emoción. En el mismo curso dieron luego su lección de despedida dos profesores que han dejado en nosotros honda huella, Rafael Manzano y José Luis Manzanares. Estos actos son un bello colofón de una carrera académica.La clase fue una reflexión sobre la arquitectura, al hilo del comentario de tres obras del arquitecto Ieoh Ming Pei en tres ciudades diferentes: las ampliaciones de la National Gallery de Washington, del Museo del Louvre de París y del Deutsches Historisches Museum de Berlín.SUMMARY The following text is a summary of my valedictory lecture, on 18 November 2006, on my retirement from the School of Architecture of Seville. As it was not recorded, and my never having written up my classes, this is a reconstruction based on my outline and the notes taken by some of those present, whom I thank for their interest.This was the first time an act of this nature was held in the School, on the initiative of the then Director, Jaime Navarro Casas. I thank him for his presence and his words, and also Juan Luis Trillo de Leyva, who made a sort of sentimental journey of my time at the School, to which I listened with emotion. Later, valedictory lectures were given by two teachers who have left a profound imprint on us, Rafael Manzano and José Luis Manzanares. These acts are a beautiful conclusion to an

  12. QCM Sensors Based on PEI Films for CO2Detection

    Institute of Scientific and Technical Information of China (English)

    Guang-Zhong Xie; Ting Kang; Yong Zhou; Tao Xie; Hui-Ling Tai; Ya-Dong Jiang

    2015-01-01

    In this paper, quartz crystal microbalance (QCM) gas sensors coated with polyehtyleneimine (PEI) was utilized for carbon dioxide (CO2) detection. The sensing mechanism is based on the availability of reversible acid-base reactions between CO2 molecules and PEI at room temperature. The experimental results revealed that the PEI/starch sensor exhibited much higher sensitivity than that of pure PEI, and showed approximate linearity over a concentration region ranging from 500ppm to 8000ppm. The influence of humidity had also been investigated. Furthermore, the response and recovery time deceased as the operation temperatures increased. Finally, sensitivity loss after conservation for several days and reversibility of the sensors had been discussed.

  13. Multiscale structural gradients enhance the biomechanical functionality of the spider fang

    Science.gov (United States)

    Bar-On, Benny; Barth, Friedrich G.; Fratzl, Peter; Politi, Yael

    2014-05-01

    The spider fang is a natural injection needle, hierarchically built from a complex composite material comprising multiscale architectural gradients. Considering its biomechanical function, the spider fang has to sustain significant mechanical loads. Here we apply experiment-based structural modelling of the fang, followed by analytical mechanical description and Finite-Element simulations, the results of which indicate that the naturally evolved fang architecture results in highly adapted effective structural stiffness and damage resilience. The analysis methods and physical insights of this work are potentially important for investigating and understanding the architecture and structural motifs of sharp-edge biological elements such as stingers, teeth, claws and more.

  14. [Professor XIAO Ji-fang's idea on acupuncture-moxibustion ].

    Science.gov (United States)

    Fan, Yu-Shan; Liao, Yu-Heng; Rui, Jing-Lin; Zhang, Cui-Zhou

    2012-03-01

    Professor XIAO Ji-fang, an experienced practitioner of Chinese medicine of Guangxi, is famous for his medical ethics, wide range of studies, comprehensive understanding of the classics, innovation, as well as u nique academic ideas. He focuses on mind-regulating in acupuncture and pulse diagnosis. At the same time, differentiation of syndromes, diseases and meridians are emphasized. The combination of acupuncture and moxibustion and integration of acupuncture and medicine are promoted. He is especially skilled at slow twisting needle insertion, Taiji needling therapy, scalp acupuncture and moxibustion with a herb prepared thread of Zhuang nationality, which all achieve remarkable effects.

  15. Bottom of the Iceberg: The Archontic Text - Review of Fanged Fan Fiction (McFarland, 2013)

    DEFF Research Database (Denmark)

    Schubart, Rikke

    2014-01-01

    Anmeldelse af Lindgren, Maria og Malin Isaksson, Fanged Fan Fiction: Variations on Twilight, True Blood and The Vampire Diaries (McFarland, 2013)......Anmeldelse af Lindgren, Maria og Malin Isaksson, Fanged Fan Fiction: Variations on Twilight, True Blood and The Vampire Diaries (McFarland, 2013)...

  16. Psychoanalysis of Jack London's "The Call of the Wild" and "White Fang"

    Science.gov (United States)

    Yang, Hongyan

    2015-01-01

    "The Call of the Wild" and "White Fang" both are masterpieces of Jack London. The protagonists Buck and White Fang are the incarnation of Jack himself to some extent for the two novels reveal a great deal of the writer. This essay aims at psychoanalyzing Jack London's creative process, the Oedipus complex and the confliction…

  17. Optimization of conditions for gene delivery system based on PEI

    Directory of Open Access Journals (Sweden)

    Roya Cheraghi

    2017-01-01

    Full Text Available Objective(s: PEI based nanoparticle (NP due to dual capabilities of proton sponge and DNA binding is known as powerful tool for nucleic acid delivery to cells. However, serious cytotoxicity and complicated conditions, which govern NPs properties and its interactions with cells practically, hindered achievement to high transfection efficiency. Here, we have tried to optimize the properties of PEI/ firefly luciferase plasmid complexes and cellular condition to improve transfection efficiency. Materials and Methods: For this purpose, firefly luciferase, as a robust gene reporter, was complexed with PEI to prepare NPs with different size and charge. The physicochemical properties of nanoparticles were evaluated using agarose gel retardation and dynamic light scattering.  MCF7 and BT474 cells at different confluency were also transfected with prepared nanoparticles at various concentrations for short and long times. Results: The branched PEI can instantaneously bind to DNA and form cationic NPs. The results demonstrated the production of nanoparticles with size about 100-500 nm dependent on N/P ratio. Moreover, increase of nanoparticles concentration on the cell surface drastically improved the transfection rate, so at a concentration of 30 ng/ìl, the highest transfection efficiency was achieved. On the other side, at confluency between 40-60%, the maximum efficiency was obtained. The result demonstrated that N/P ratio of 12 could establish an optimized ratio between transfection efficiency and cytotoxicity of PEI/plasmid nanoparticles. The increase of NPs N/P ratio led to significant cytotoxicity. Conclusion: Obtained results verified the optimum conditions for PEI based gene delivery in different cell lines.

  18. Synthesizing Iron Oxide Nanostructures: The Polyethylenenemine (PEI Role

    Directory of Open Access Journals (Sweden)

    Sergio Lentijo Mozo

    2017-01-01

    Full Text Available Controlled synthesis of anisotropic iron oxide nanoparticles is a challenge in the field of nanomaterial research that requires an extreme attention to detail. In particular, following up a previous work showcasing the synthesis of magnetite nanorods (NRs using a two-step approach that made use of polyethylenenemine (PEI as a capping ligand to synthesize intermediate β-FeOOH NRs, we studied the effect and influence of the capping ligand on the formation of β-FeOOH NRs. By comparing the results reported in the literature with those we obtained from syntheses performed (1 in the absence of PEI or (2 by using PEIs with different molecular weight, we showed how the choice of different PEIs determines the aspect ratio and the structural stability of the β-FeOOH NRs and how this affects the final products. For this purpose, a combination of XRD, HRTEM, and direct current superconducting quantum interference device (DC SQUID magnetometry was used to identify the phases formed in the final products and study their morphostructural features and related magnetic behavior.

  19. Synthesizing Iron Oxide Nanostructures: The Polyethylenenemine (PEI) Role

    KAUST Repository

    Mozo, Sergio Lentijo

    2017-01-12

    Controlled synthesis of anisotropic iron oxide nanoparticles is a challenge in the field of nanomaterial research that requires an extreme attention to detail. In particular, following up a previous work showcasing the synthesis of magnetite nanorods (NRs) using a two-step approach that made use of polyethylenenemine (PEI) as a capping ligand to synthesize intermediate β-FeOOH NRs, we studied the effect and influence of the capping ligand on the formation of β-FeOOH NRs. By comparing the results reported in the literature with those we obtained from syntheses performed (1) in the absence of PEI or (2) by using PEIs with different molecular weight, we showed how the choice of different PEIs determines the aspect ratio and the structural stability of the β-FeOOH NRs and how this affects the final products. For this purpose, a combination of XRD, HRTEM, and direct current superconducting quantum interference device (DC SQUID) magnetometry was used to identify the phases formed in the final products and study their morphostructural features and related magnetic behavior.

  20. Modifying the adsorption properties of anionic surfactants onto hydrophilic silica using the pH dependence of the polyelectrolytes PEI, ethoxylated PEI, and polyamines.

    Science.gov (United States)

    Zhang, Xiaoli; Taylor, Diana; Thomas, Robert; Penfold, Jeffrey; Tucker, Ian

    2011-04-05

    The manipulation of the adsorption of the anionic surfactant, sodium dodecyl sulfate, SDS, onto hydrophilic silica by the polyelectrolytes, polyethyleneimine, PEI, ethoxylated PEI, and the polyamine, pentaethylenehexamine, has been studied using neutron reflectometry. The adsorption of a thin PEI layer onto hydrophilic silica promotes a strong reversible adsorption of the SDS through surface charge reversal induced by the PEI at pH 7. At pH 2.4, a much thicker adsorbed PEI layer is partially swelled by the SDS, and the SDS adsorption is now no longer completely reversible. At pH 10, there is some penetration of SDS and solvent into a thin PEI layer, and the SDS adsorption is again not fully reversible. Ethoxylation of the PEI (PEI-EO(1) and PEI-EO(7)) results in a much weaker and fragile PEI and SDS adsorption at both pH 3 and pH 10, and both polymer and surfactant desorb at higher surfactant concentrations (>critical micellar concentration, cmc). For the polyamine, pentaethylenehexamine, adsorption of a layer of intermediate thickness is observed at pH 10, but at pH 3, no polyamine adsorption is evident; and at both pH 3 and pH 10, no SDS adsorption is observed. The results presented here show that, for the amine-based polyelectrolytes, polymer architecture, molecular weight, and pH can be used to manipulate the surface affinity for anionic surfactant (SDS) adsorption onto polyelectrolyte-coated hydrophilic silica surfaces.

  1. Understanding Biological Roles of Venoms Among the Caenophidia: The Importance of Rear-Fanged Snakes.

    Science.gov (United States)

    Mackessy, Stephen P; Saviola, Anthony J

    2016-11-01

    Snake venoms represent an adaptive trophic response to the challenges confronting a limbless predator for overcoming combative prey, and this chemical means of subduing prey shows several dominant phenotypes. Many front-fanged snakes, particularly vipers, feed on various vertebrate and invertebrate prey species, and some of their venom components (e.g., metalloproteinases, cobratoxin) appear to have been selected for "broad-brush" incapacitation of different prey taxa. Using proteomic and genomic techniques, the compositional diversity of front-fanged snakes is becoming well characterized; however, this is not the case for most rear-fanged colubroid snakes. Because these species consume a high diversity of prey, and because venoms are primarily a trophic adaptation, important clues for understanding specific selective pressures favoring venom component composition will be found among rear-fanged snake venoms. Rear-fanged snakes typically (but not always) produce venoms with lower complexity than front-fanged snakes, and there are even fewer dominant (and, arguably, biologically most relevant) venom protein families. We have demonstrated taxon-specific toxic effects, where lizards and birds show high susceptibility while mammals are largely unaffected, for both Old World and New World rear-fanged snakes, strongly indicating a causal link between toxin evolution and prey preference. New data are presented on myotoxin a, showing that the extremely rapid paralysis induced by this rattlesnake toxin is specific for rodents, and that myotoxin a is ineffectual against lizards. Relatively few rear-fanged snake venoms have been characterized, and basic natural history data are largely lacking, but directed sampling of specialized species indicates that novel compounds are likely among these specialists, particularly among those species feeding on invertebrate prey such as scorpions and centipedes. Because many of the more than 2200 species of colubroid snakes are rear-fanged

  2. San luo xuan mo xing ji qi liang hua fen zhi fang fa yan tao

    NARCIS (Netherlands)

    Ye, Y.; Leydesdorff, L.; Wu, Y.

    2014-01-01

    A discussed study on the Triple Helix with its quantitative analysis method. With brief introduction to background and progress of Triple-Helix(TH) researches, it is clarified that TH quantitative methodology focuses on the indicator of synergy (T) as reduction of uncertainty that can be shaped amon

  3. An experimental investigation of Fang's Ag superlens suitable for integration

    DEFF Research Database (Denmark)

    Jeppesen, Claus; Nielsen, Rasmus Bundgaard; Xiao, Sanshui

    2009-01-01

    We report on experimental realization of the Fang Ag superlens structure [1] suitable for further processing and integration in bio-chips by replacing PMMA with a highly chemical resistant cyclo-olefin copolymer, mr-I T85 (Micro Resist Technology, Berlin, Germany). The superlens was able to resolve...... processing is needed involving various organic polar solvents, PMMA cannot be used. We propose to use mr-I T85, which is highly chemically resistant to acids and polar solvents. Our superlens stack consists of a quartz/chrome grating mask, a 40 nm layer of mr-I T85, 35 nm Ag, and finally 70 nm...... to the excitation wavelength of the surface plasmons at the mr-I T85/Ag interface. Supporting COMSOL simulations illustrate the field intensity distribution inside the resist as well as the presence of surface plasmons at the mr-I T85/Ag boundary. AFM scans of the exposed structure revealed 80 nm gratings....

  4. Acetylcholinesterase immobilized onto PEI-coated silica nanoparticles.

    Science.gov (United States)

    Tumturk, Hayrettin; Yüksekdag, Hazer

    2016-01-01

    Polyethyleneimine (PEI) coated-silica nanoparticles were prepared by the Stöber method. The formation and the structure of the nanoparticles were characterized by ATR-FT-IR spectroscopy and transmission electron microscopy (TEM). TEM images of the silica and PEI-coated nanoparticles revealed that they were well dispersed and that there was no agglomeration. The acetylcholineesterase enzyme was immobilized onto these nanoparticles. The effects of pH and temperature on the storage stability of the free and immobilized enzyme were investigated. The optimum pHs for free and immobilized enzymes were determined as 7.0 and 8.0, respectively. The optimum temperatures for free and immobilized enzymes were found to be 30.0 and 35.0°C, respectively. The maximum reaction rate (Vmax) and the Michaelis-Menten constant (Km) were investigated for the free and immobilized enzyme. The storage stability of acetylcholinesterase was increased when immobilized onto the novel PEI-coated silica nanoparticles. The reuse numbers of immobilized enzyme were also studied. These hybrid nanoparticles are desirable as carriers for biomedical applications.

  5. Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos

    DEFF Research Database (Denmark)

    Zhou, Xiang; Laroche, Fabrice Jean Francois; Lamers, Gerda E M

    2012-01-01

    of DNA-condensing compounds required for efficient uptake of DNA. Here we report a polyethyleneimine (PEI) grafted ultra-small graphene oxide (PEI-g-USGO) for transfection. By removing the free PEI and ensuring a high PEI density on small sized graphene, we obtained very high transfection efficiencies...

  6. Role of Additives in Composite PEI/Oxide CO₂ Adsorbents: Enhancement in the Amine Efficiency of Supported PEI by PEG in CO₂ Capture from Simulated Ambient Air.

    Science.gov (United States)

    Sakwa-Novak, Miles A; Tan, Shuai; Jones, Christopher W

    2015-11-11

    Supported amines are promising candidate adsorbents for the removal of CO2 from flue gases and directly from ambient air. The incorporation of additives into polymeric amines such as poly(ethylenimine) (PEI) supported on mesoporous oxides is an effective strategy to improve the performance of the materials. Here, several practical aspects of this strategy are addressed with regards to direct air capture. The influence of three additives (CTAB, PEG200, PEG1000) was systematically explored under dry simulated air capture conditions (400 ppm of CO2, 30 °C). With SBA-15 as a model support for poly(ethylenimine) (PEI), the nature of the additive induced heterogeneities in the deposition of organic on the interior and exterior of the particles, an important consideration for future scale up to practical systems. The PEG200 additive increased the observed thermodynamic performance (∼60% increase in amine efficiency) of the adsorbents regardless of the PEI content, while the other molecules had less positive effects. A threshold PEG200/PEI value was identified at which the diffusional limitations of CO2 within the materials were nearly eliminated. The threshold PEG/PEI ratio may have physical origin in the interactions between PEI and PEG, as the optimal ratio corresponded to nearly equimolar OH/reactive (1°, 2°) amine ratios. The strategy is shown to be robust to the characteristics of the host support, as PEG200 improved the amine efficiency of PEI when supported on two varieties of mesoporous γ-alumina with PEI.

  7. F-127-PEI co-delivering docetaxel and TFPI-2 plasmid for nasopharyngeal cancer therapy

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Tao, E-mail: taoliu18@126.com [Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282 (China); Zhang, Xinyu [Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282 (China); Ke, Bo [Jiangxi Key Laboratory of Hematological Oncology and Cell Biology, Jiangxi Provincial People' s Hospital, Nanchang 330006 (China); Wang, Yigang [School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018 (China); Wu, Xidong [Department of Pharmacology, Jiangxi Institute of Materia Medica,Nanchang 330029 (China); Jiang, Gang [Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282 (China); Wu, Ting [Department of Light Chemical Engineering, Guangdong Polytechnic, Foshan 528041 (China); Nie, Guohui, E-mail: nghui@21cn.com [Department of Otolaryngological, Peking University Shenzhen Hospital, Shenzhen 518036 (China)

    2016-04-01

    The co-delivery of drug and gene has become the primary strategy in cancer therapy. However, to construct one safe co-delivering system with higher drug loading and gene transfection efficiency for cancer therapy is still challenging. Herein, a novel degradable nanocarriers were synthesized and characterized in this study, which was composed of polyethylenimine (PEI)-linked PEO–PPO–PEO (Pluronic F127), called F127-PEI. Then the nanocarrier was used for hydrophobic docetaxel (DOC) and functional gene (TFPI-2 plasmid) co-delivery to treat nasopharyngeal cancer (NPC). The results indicated that F127-PEI nanocarriers had higher DOC loading amount and possessed good gene delivery effect in vitro. For co-delivery analysis, the obtained F127-PEI/DOC/TFPI-2 complexes could induce a more significant apoptosis than DOC or TFPI-2 alone, and decreased invasive capacity of NPC HNE-1 cells more obviously. Moreover, the F127-PEI copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k by the hemolysis and MTT assays, which suggests a promising potential for NPC therapy. - Highlights: • F127-PEI was synthesized and used for drug and gene co-delivery. • F127-PEI showed good delivery ability to docetaxel and TFPI-2 plasmid. • The co-loaded complexes showed synergistic effect to nasopharyngeal carcinoma. • F127-PEI showed better blood safety and lower cytotoxicity compared to PEI-25k.

  8. [Needling technique of Professor Li Yan-Fang].

    Science.gov (United States)

    Li, Li-Jun

    2014-01-01

    Experiences of needling techniques of Professor LI Ya- fang is introduced in this article. Gentle and superficial insertion is adopted by Professor LI in clinic. Emphases are put on the qi regulation function, needling sensation to the affected region and insertion with both hands, especially the function of the left hand as pressing hand. The gentle and superficial insertion should be done as the follows: hold the needle with the right hand, press gently along the running course of meridians with the left hand to promote qi circulation, hard pressing should be applied at acupoints to disperse the local qi and blood, insert the needle gently and quickly into the subcutaneous region with the right hand, and stop the insertion when patient has the needling sensation. While the fast needling is characterized with shallow insertion and swift manipulation: the left hand of the manipulator should press first along the running course of the meridian, and fix the local skin, hold the needle with the right hand and insert the needle quickly into the acupoint. Withdrawal of the needle should be done immediately after the reinforcing and reducing manipulations. Professor LI is accomplished in qi regulation. It is held by him that regulating qi circulation is essence of acupuncture, letting the patient get the needling sensation is the most important task of needling. Lifting, thrusting and rotation manipulations should be applied to do reinforcing or reducing. The tissue around the tip of the needle should not be too contracted or too relaxed, and the resistance should not be too strong or too weak. The feeling of the insertion hand of the practitioner should not be too smooth or too hesitant. Needle should be inserted into the skin quickly at the moment of hard pressing by the left hand. And then, slow rotation and gentle lifting and thrusting can be applied to promote the needling sensation like electric current pass through and to reach the affected region along the

  9. Anti-depression effect of Kai Xin San and research of its compatibility

    Institute of Scientific and Technical Information of China (English)

    CHEN Yi-hong; WANG Dong-xiao; LIU Ping; WANG Jin-liang; DAI Chang; CHENG Gui-fang

    2005-01-01

    @@ Kai Xin San (KXS) is a category of traditional complex prescription that was first recorded in Bei Ji Qian Jin Yao Fang by SUN Si-miao in the Tang Dynasty and then also recorded in Tai Ping Hui Min He Ji Ju Fang, Qian Jin Yao Fang and Gu Jin Lu Yan in later generations. It was mainly used by herbalist doctors to treat symptoms including desolation, moodiness, forgetfulness etc., which are similar to the neurosis such as depression, anxiety and difficulty in studying and memory in the West medicine. The former recorded formulas were all composed of ginseng, polygala, hoelen and acori graminei with different proportion. But there are no systematic studies on the clinical adaptive symptom corresponding to different matching and the best anti-depressive matching so far.

  10. Synthesis of Pt/PEI-MWCNT composite materials on polyethyleneimine-functionalized MWNTs as supports

    Energy Technology Data Exchange (ETDEWEB)

    Yi, Sung-Chul; Jung, Chi Young [Department of Chemical Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul 133-791 (Korea, Republic of); Kim, Wha Jung, E-mail: whajungk@konkuk.ac.kr [Department of Materials Chemistry and Engineering, Konkuk University, 1-Hwayang-dong, Kwangjin-gu, Seoul 143-701 (Korea, Republic of)

    2011-12-15

    Graphical abstract: Schematic diagram for (a) the preparation of Pt/MWCNT functionalized with PEI and (b) TEM of Pt/PEI-MWCNT. Highlights: Black-Right-Pointing-Pointer We prepared Pt/PEI/MWCNT where MWCNT was first functionalized by PEI (polyethyleneimine) followed by Pt deposit onto it. Black-Right-Pointing-Pointer PEI functionalization provided high density homogeneous functional groups on MWCNT's sidewall. Black-Right-Pointing-Pointer Cationic PEI leads to homogeneous dispersion in solutions such as water and organic solvents. Black-Right-Pointing-Pointer Pt/PEI/MWCNT catalyst exhibits excellent electrocatalytic activity compared to that of Pt/MWCNT catalyst obtained with polyvinylpyrrolidine (PVP). -- Abstract: Composite materials with highly dispersed platinum (Pt) nanoparticles on multiwalled carbon nanotubes (MWCNTs), functionalized with polyethyleneimine (PEI) by a noncovalent method were prepared. The PEI-functionalization provided high density homogeneous functional groups on the MWCNTs' sidewalls for binding Pt nanoparticles. Cationic PEI leads to homogeneous dispersion in solutions such as water and organic solvents. The effects of a reducing agent on the Pt nanoparticles that form on the surfaces of the MWCNT were studied by varying the molar ratio of NaOH to H{sub 2}PtCl{sub 6}. These composite materials were characterized with transmission electron micrograph (TEM), X-ray diffractometer (XRD) and X-ray photoelectron spectroscopy (XPS). The Pt/PEI-MWCNT catalyst exhibits excellent electrocatalytic activity and compared with Pt/PVP-MWCNT catalysts obtained with polyvinylpyrrolidone (PVP). Finally, the cyclic voltammogram of methanol electrooxidation for Pt/PEI-MWCNT shows better tolerance to CO and methanol oxidation to CO{sub 2} than of Pt/PVP-MWCNT.

  11. Feasibility of Gas Drive in Fang-48 Fault Block Oil Reservoir

    Institute of Scientific and Technical Information of China (English)

    Cui Lining; Hou Jirui; Yin Xiangwen

    2007-01-01

    The Fang-48 fault block oil reservoir is an extremely low permeability reservoir, and it is difficult to produce such a reservoir by waterflooding. Laboratory analysis of reservoir oil shows that the minimum miscibility pressure for CO2 drive in Fang-48 fault block oil reservoir is 29 MPa, lower than the formation fracture pressure of 34 MPa, so the displacement mechanism is miscible drive. The threshold pressure gradient for gas injection is less than that for waterflooding, and the recovery by gas drive is higher than waterflooding. Furthermore, the threshold pressure gradient for carbon dioxide injection is smaller than that for hydrocarbon gas, and the oil recovery by carbon dioxide drive is higher than that by hydrocarbon gas displacement, so carbon dioxide drive is recommended for the development of the Fang-48 fault block oil reservoir.

  12. Effects of Huo Nao Fang in 60 Cases of Ischemic Apoplexy

    Institute of Scientific and Technical Information of China (English)

    陈尚书

    2003-01-01

    To observe the therapeutic effects of the prescription of Huo Nao Fang (活脑方) on ischemic apoplexy,120 patients were divided into a treatment group and a control group, with 60 in each group, the formertreated with Huo Nao Fang while the latter with western medicine. The nervous function and bloodrheology were tested before and after treatment for both groups, and the effects of Huo Nao Fang wereevaluated. The results showed that the total effective rate was 91.7% in the treatment group and 61.6% inthe control group, with a significant difference (P<0.01) between the two groups. The treatment group wassuperior to the control group in improving blood rheology, reducing blood lipid and restoring the nervousfunction (P<0.05).

  13. Linear and Branched PEIs (Polyethylenimines and Their Property Space

    Directory of Open Access Journals (Sweden)

    Claudiu N. Lungu

    2016-04-01

    Full Text Available A chemical property space defines the adaptability of a molecule to changing conditions and its interaction with other molecular systems determining a pharmacological response. Within a congeneric molecular series (compounds with the same derivatization algorithm and thus the same brute formula the chemical properties vary in a monotonic manner, i.e., congeneric compounds share the same chemical property space. The chemical property space is a key component in molecular design, where some building blocks are functionalized, i.e., derivatized, and eventually self-assembled in more complex systems, such as enzyme-ligand systems, of which (physico-chemical properties/bioactivity may be predicted by QSPR/QSAR (quantitative structure-property/activity relationship studies. The system structure is determined by the binding type (temporal/permanent; electrostatic/covalent and is reflected in its local electronic (and/or magnetic properties. Such nano-systems play the role of molecular devices, important in nano-medicine. In the present article, the behavior of polyethylenimine (PEI macromolecules (linear LPEI and branched BPEI, respectively with respect to the glucose oxidase enzyme GOx is described in terms of their (interacting energy, geometry and topology, in an attempt to find the best shape and size of PEIs to be useful for a chosen (nanochemistry purpose.

  14. Linear and Branched PEIs (Polyethylenimines) and Their Property Space

    Science.gov (United States)

    Lungu, Claudiu N.; Diudea, Mircea V.; Putz, Mihai V.; Grudziński, Ireneusz P.

    2016-01-01

    A chemical property space defines the adaptability of a molecule to changing conditions and its interaction with other molecular systems determining a pharmacological response. Within a congeneric molecular series (compounds with the same derivatization algorithm and thus the same brute formula) the chemical properties vary in a monotonic manner, i.e., congeneric compounds share the same chemical property space. The chemical property space is a key component in molecular design, where some building blocks are functionalized, i.e., derivatized, and eventually self-assembled in more complex systems, such as enzyme-ligand systems, of which (physico-chemical) properties/bioactivity may be predicted by QSPR/QSAR (quantitative structure-property/activity relationship) studies. The system structure is determined by the binding type (temporal/permanent; electrostatic/covalent) and is reflected in its local electronic (and/or magnetic) properties. Such nano-systems play the role of molecular devices, important in nano-medicine. In the present article, the behavior of polyethylenimine (PEI) macromolecules (linear LPEI and branched BPEI, respectively) with respect to the glucose oxidase enzyme GOx is described in terms of their (interacting) energy, geometry and topology, in an attempt to find the best shape and size of PEIs to be useful for a chosen (nanochemistry) purpose. PMID:27089324

  15. Linear and Branched PEIs (Polyethylenimines) and Their Property Space.

    Science.gov (United States)

    Lungu, Claudiu N; Diudea, Mircea V; Putz, Mihai V; Grudziński, Ireneusz P

    2016-04-13

    A chemical property space defines the adaptability of a molecule to changing conditions and its interaction with other molecular systems determining a pharmacological response. Within a congeneric molecular series (compounds with the same derivatization algorithm and thus the same brute formula) the chemical properties vary in a monotonic manner, i.e., congeneric compounds share the same chemical property space. The chemical property space is a key component in molecular design, where some building blocks are functionalized, i.e., derivatized, and eventually self-assembled in more complex systems, such as enzyme-ligand systems, of which (physico-chemical) properties/bioactivity may be predicted by QSPR/QSAR (quantitative structure-property/activity relationship) studies. The system structure is determined by the binding type (temporal/permanent; electrostatic/covalent) and is reflected in its local electronic (and/or magnetic) properties. Such nano-systems play the role of molecular devices, important in nano-medicine. In the present article, the behavior of polyethylenimine (PEI) macromolecules (linear LPEI and branched BPEI, respectively) with respect to the glucose oxidase enzyme GOx is described in terms of their (interacting) energy, geometry and topology, in an attempt to find the best shape and size of PEIs to be useful for a chosen (nanochemistry) purpose.

  16. "Chi-Fang-Qi": Is it the aureole of an ancient supernova?

    Institute of Scientific and Technical Information of China (English)

    ZHAO Fuyuan; JIANG Shiyang

    2005-01-01

    An annotation for the statement about JingXing (Great Star) in the most famous Chinese ancient historical book Shi Ji (Historical Records) authored by Sima Qian reads as follows: "There was a red aureole ("Chi-Fang-Qi") which connected with a deep-color aureole ("Qing-Fang-Qi"). Two yellow stars were in the red aureole,one yellow star was in the deep-color aureole". The author of the annotation was Meng Kang, an officer as well as a scholar of the Wei State in the Three Kingdoms period. The same records are to be found in two other Chinese historical books compiled by the authors of later ages in Tang Dynasty --Jin History and Sui History. The scene illustrated by the record of "Chi-Fang-Qi" is very similar to the pictures of SN1987A taken by the Hubble Space Telescope. Let it be noted that although the ancient authors were often with serious wrong understandings of some astronomical phenomena such as supernovae, their depictions about natural phenomena they witnessed were mostly objective, and it was unlikely that they fictionally made the record of "Chi-Fang-Qi". We infer that the ancient observers might have ever watched a very bright supernova with an aureole structure in its remnant and then left such a record. Meng Kang perhaps was the first to give the record about "Chi-Fang-Qi", and he might have lived in the period from about AD 180 to AD250, very near to AD 185, so, here the supernova the ancient people watched was most likely the one in the year of AD 185. This supposition is consistent with the distance parameter of the most possible remnant of SN185 derived by some modern measurements. If the correlation between the record of "Chi-Fang-Qi" and the supernova remnant can be further proven true, it would be an important verification of the modern theory about stellarevolution.

  17. [Experience of professor FANG Jianqiao treating trigeminal neuralgia at different stages].

    Science.gov (United States)

    Sun, Jing; Fang, Jianqiao; Shao, Xiaomei; Chen, Lifang

    2016-02-01

    Trigerninal neuralgia is a common refractory disease in clinic. Professor FANG Jianqiao has rich experience through diagnosing and treating the disease for many years. In the first diagnosis, professor FANG underlines the position of damaged neuron and syndrome differentiation. He considers acupuncture should be implemented by stages,namely according to whether the patients are in the period of pain attack, different acupuocture prescriptions are made. Acupuncture manipulation and needle-retention time should be adjusted according to the condition of disease. And the appropriate application of electroacupuncture and transcutaneous electrical acupoint stimulation can strengthen the effect.

  18. Changes in the Narrative of Fang Fang's Novels in the New Century%方方新世纪小说的叙事变化

    Institute of Scientific and Technical Information of China (English)

    王达敏

    2012-01-01

    As one of the most influential and outstanding contemporary Chinese writers, Fang Fang distin- guished himself in the literary circles in the 1980s and 1990s by "originally" portraying the coarse living state of common people in a secular stance as well as the deceit, suppression and alienation of human beings by the rigid and vulgar social reality, thereby becoming a representative writer of "neo-realism". In his novels of the new cen- tury, Fang Fang has realized his transcendence, as is manifest in the change of his narrative, i.e. the change from the portrayal of the survival dilemma of human beings and the alienation of humanity to the depiction of suh contents as "the survival destiny of human beings". "the confusion of humanitv" and "humanism" etc.%方方是中国当代最有影响力的优秀小说家之一。上世纪八九十年代,方方曾以世俗立场“原生态”地描写普通百姓粗鄙化的生存现状,以及僵化庸俗的现实对人的愚弄、迫压和异化而享誉文坛,成为“新写实”派代表性作家。在新世纪小说创作中,方方实现了对自己的超越,体现为叙事的变化,即从描写入的生存困境和人性异化到表现“人的生存命运”、“人性之困”和“人道主义”等思想内容。

  19. Temperature, salinity, sigma_t, pressure measurement collected using CTD from an unknown platform in the Min Fang Bay from 1984 to 1985 (NODC Accession 0048830)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Southern Ocean data - Min Fang Bay , temperature and salinity measurements collected using CTD from unknown platform in the Min Fang Bay from 1984 to 1985

  20. Synthesis of Superparamagnetic Iron Oxide Nanoparticles Modified with MPEG-PEI via Photochemistry as New MRI Contrast Agent

    Directory of Open Access Journals (Sweden)

    Yancong Zhang

    2015-01-01

    Full Text Available Novel method for synthesis of superparamagnetic iron oxide nanoparticles (SPIONs coated with polyethylenimine (PEI and modified with poly(ethylene glycol methyl ether (MPEG, MPEG-PEI-SPIONs, was developed. PEI-SPIONs were successfully prepared in aqueous system via photochemistry, and their surface was modified with poly(ethylene glycol methyl ether (MPEG. The so-obtained MPEG-PEI-SPIONs had a uniform hydrodynamic particle size of 34 nm. The successful coating of MPEG-PEI on the SPIONs was ascertained from FT-IR analysis, and the PEI and MPEG fractions in MPEG-PEI-SPIONs were calculated to account for 31% and 12%, respectively. Magnetic measurement revealed that the saturated magnetization of MPEG-PEI-SPIONs reached 46 emu/g and the nanoparticles showed the characteristic of being superparamagnetic. The stability experiment revealed that the MPEG-PEI modification improved the nanoparticles stability greatly. T2 relaxation measurements showed that MPEG-PEI-SPIONs show similar R2 value to the PEI-SPIONs. The T2-weighted magnetic resonance imaging (MRI of MPEG-PEI-SPIONs showed that the magnetic resonance signal was enhanced significantly with increasing nanoparticle concentration in water. These results indicated that the MPEG-PEI-SPIONs had great potential for application in MRI.

  1. San Francisco District Laboratory (SAN)

    Data.gov (United States)

    Federal Laboratory Consortium — Program Capabilities Food Analysis SAN-DO Laboratory has an expert in elemental analysis who frequently performs field inspections of materials. A recently acquired...

  2. Solution Behavior of Modified Polyethylenimine (PEI) Polymers by Light Scattering Investigations

    Energy Technology Data Exchange (ETDEWEB)

    Sonny A. Ekhorutomwen; Samuel P. Sawan; Barbara F. Smith; Thomas W. Robison; Kennard V. Wilson

    2004-03-18

    The eight average molecular weights, as well as other characteristics such as the second virial coefficients and root-mean-square (RMS) radii of gyration of poly (ethyleneimine) (PEI) and various derivatives, have been determined in solution light scattering studies. The solution dynamics of PEI and carboxylated and phosphorylated derivatives were studied a pH of 3.3, 7.0 and 10.0. Measurements were made in freshly distilled and de-ionized water as well as in 0.1 M, 1 M and 5-M solutions of sodium chloride in water. Molecular weights were calculated from Berry plots. The purified polymer, PEI-1, gave a molecular weight of 39,600 g/mol., while the same polymer, which was not purified, PEI-2, has MW of 43,100 g/mol.

  3. [Release characteristics in vitro and pharmacokinetics of da chuanxiong fang multiunit drug delivery system in rats].

    Science.gov (United States)

    Wei, Yuan-feng; Zhang, Ning; Lin, Xiao; Feng, Yi

    2011-09-01

    The drug release characteristics ofDa Chuanxiong Fang multiunit drug delivery system (DCXFMDDS) in vivo and in vitro were evaluated. Ferulic acid (FA) and senkyunolide I (SI) were used as marker components, which were two of the effective components of Da Chuanxiong Fang. And their contents were determined by HPLC. Drug release characteristics in vitro of DCXFMDDS and Da Chuanxiong pills and pharmacokinetics characteristics of DCXFMDDS and Da Chuanxiong Fang active fraction (DCXFAF) in rats were compared. It was obvious that FA released from the DCXFMDDS in a sustained fashion but SI in a fast fashion both in vitro and in vivo. The releasing process and the releasing mechanism of FA and SI from DCXFMDDS were different, but the AUC value indicated that compared with DCXFAF the extent of absorption of FA and SI from DCXFMDDS was increased. Though from the same multiunit drug delivery system, FA an SI had different drug release characteristics both in vitro and in vivo, and that may be one of the reason why DCXFMDDS has the good properties such as rapid and long-lasting effect and high efficiency.

  4. Analysis of Fang Puncture Wound Patterns in Isfahan Province’s, Iran, Venomous and Non-Venomous Snakes

    Directory of Open Access Journals (Sweden)

    Dehghani R.1 PhD,

    2015-01-01

    Full Text Available Aims Venomous snake bites are public health problems in different parts of the world. The most specific mainstay in the treatment of envenomation is anti-venom. To treat the envenomation, it is very important to identify the offending species. This study was designed to determine the penetrating pattern of fangs and teeth of some viper snakes. Materials & Methods This descriptive study was performed on live venomous and nonvenomous snakes from 2010 till 2011. All 47 sample snakes were collected from different regions of Isfahan province such as Kashan City, Ghamsar, Niasar, Mashhad Ardehal, Taher- Abad and Khozagh. Their mouths were inspected every two weeks and development of their fangs and teeth were recorded by taking clear digital photos. Fangs and teeth patterns of samples were drawn and the results were compared. Findings One or two wounds appeared as typical fang marks at the bite site of venomous snakes while non-venomous snakes had two carved rows of small teeth. Three different teeth and fang patterns were recognized in venomous snakes which were completely different. Conclusion The fang marks of venomous snakes do not always have a common and classic pattern and there are at least 3 different patterns in Isfahan province, Iran.

  5. BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector

    Science.gov (United States)

    Yue, Jianhui; Wu, Jun; Liu, Di; Zhao, Xiaoli; Lu, William W.

    2015-04-01

    Nanotechnology has made a significant impact on the development of nanomedicine. Nonviral vectors have been attracting more attention for the advantage of biosafety in gene delivery. Polyethylenimine (PEI)-conjugated chitosan (chitosan-g-PEI) emerged as a promising nonviral vector and has been demonstrated in many tumor cells. However, there is a lack of study focused on the behavior of this vector in stem cells which hold great potential in regenerative medicine. Therefore, in this study, in vitro gene delivering effect of chitosan-g-PEI was investigated in bone marrow stem cells. pIRES2-ZsGreen1-hBMP2 dual expression plasmid containing both the ZsGreen1 GFP reporter gene and the BMP2 functional gene was constructed for monitoring the transgene expression level. Chitosan-g-PEI-mediated gene transfer showed 17.2% of transfection efficiency and more than 80% of cell viability in stem cells. These values were higher than that of PEI. The expression of the delivered BMP2 gene in stem cells enhanced the osteogenic differentiation. These results demonstrated that chitosan-g-PEI is capable of applying in delivering gene to stem cells and providing potential applications in stem cell-based gene therapy.

  6. San Francisco District Laboratory (SAN)

    Data.gov (United States)

    Federal Laboratory Consortium — Program CapabilitiesFood Analysis SAN-DO Laboratory has an expert in elemental analysis who frequently performs field inspections of materials. A recently acquired...

  7. Suspeita de febre familiar dos cães Shar-peis chineses Suspected familiar chinese Shar-pei dog fever

    Directory of Open Access Journals (Sweden)

    Marcia Regina da Silva Ilha

    2005-06-01

    Full Text Available A febre (síndrome familiar dos cães Shar-peis Chineses é uma doença hereditária autossômica recessiva. Este trabalho descreve um caso de amiloidose generalizada em um cão Shar-pei Chinês, fêmea, de três anos de idade, cujo quadro clínico-patológico é consistente com o da febre familiar dos cães Shar-peis chineses. Esse animal tinha uma história clínica de edema bilateral recidivante na região do jarrete que vinha sendo observado desde os sete meses de idade. Três dias antes da morte, esse cão apresentou anorexia, apatia, vômito e diarréia. À necropsia, ambos os rins estavam firmes, pálidos e possuíam a superfície capsular irregular. Histologicamente foram observados depósitos de amilóide nos rins, no pâncreas, na tireóide e no baço. Esse amilóide perdeu sua afinidade para o vermelho Congo, quando utilizado o permanganato de potássio. Esse achado é consistente com amiloidose do tipo AA.Chinese Shar-pei familial fever is a breed-associated hereditary autosomal recessive disease of Chinese Shar-pei dogs. A case of generalized amyloidosis in a 3-year-old female Chinese Shar-pei dog with a tentative diagnosis of familial Chinese Shar-pei fever is described. This animal had a clinical history of episodic bilateral swelling of the tarsal joints since 7-month-old. Vomiting, diarrhea, anorexia and lethargy were observed 3 days prior to death. At necropsy, both kidneys were firm, whitish and had an irregular capsular surface. Microscopically, amyloid deposits were observed in the kidneys, pancreas, thyroid glands and spleen. Amyloid deposits lost their affinity for Congo red after treatment with potassium permanganate indicating AA-type amyloidosis.

  8. [Aspirin-PEI-beta-CyD as a novel non-viral vector for gene transfer].

    Science.gov (United States)

    Wang, Zhong-Ren; Chen, Dan; Zhou, Jun; Tang, Gu-Ping

    2009-01-01

    To develop a novel non-viral gene delivery vector based on PEI-beta-CyD as backbone modified with aspirin, and to identify its physicochemical characters. 1, 1-carbonyldiimidazole (CDI) was used to bind aspirin onto PEI-beta-CyD to form PEI-beta-CyD-ASP. (1)H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16, Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 cells. The structure of PEI-beta-CyD-ASP was confirmed by (1)H-NMR, FT-IR, UV and XRD, which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells. The synthesized aspirin-PEI-beta-CyD might be a potential gene delivery vector.

  9. Bioreducible crosslinked low molecular weight branched PEI-PBLG as an efficient gene carrier

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Low molecular weight polyethylenimine-poly(gamma-benzyl L-glutamate) (PEI-PBLG) was crosslinked by N,N’-cystaminebisacrylamide (CBA) to get the polymer named as CBA-PEI-PBLG (CPP).CPP not only inherits PEI-PBLG’s amphiphilic advantages,but also possesses reducible properties.CPP can complex with DNA to form nanoparticles.CPP/DNA complex particles were characterized by particle size and zeta potential analysis.The result showed that the complex particles have suitable size and surface charges for gene delivery.And gel retardation assay also prove CBA-PEI-PBLG has proper condensing ability for DNA.CPP has good reducible property,and also has good biocompatibility because of introducing PBLG segment.The cytotoxicity of CPP was evaluated using MTT assay,and the results showed CPP has lower cytotoxicity compared with PEI 25 K.The transfection properties were characterized in different cells by using plasmid DNA as a reporter.CPP showed higher transfection efficiencies and lower cytotoxicity in HeLa cells.This was attributed to bioreducible and biocompatibility properties of the CPP.These results suggested that CPP is a promising low-toxic,highly effective non-viral gene carrier.

  10. Preparation of PVA/PEI ultra-fine fibers and their composite membrane with PLA by electrospinning.

    Science.gov (United States)

    Dong, Cunhai; Yuan, Xiaoyan; He, Mingyu; Yao, Kangde

    2006-01-01

    Ultra-fine fibers of poly(vinyl alcohol)/polyethylenimine (PVA/PEI) were prepared by electrospinning of their blend solutions in water. Effects of PVA/PEI mass ratio and the polymer concentration on the fiber morphology were discussed by analysis of scanning electron micrographs. Results showed that uniform ultra-fine fibers could be obtained from an 8% PVA/PEI solution with 75:25 mass ratio. It was supposed that the introduction of PVA could promote electrospinning of PEI by weakening the intermolecular interaction and increasing solution viscosity. A composite membrane of PVA/PEI with poly(D,L-lactide) (PLA) was produced by co-electrospinning simultaneously from the aqueous 8% PVA/PEI (75:25) solution and a 20% PLA solution in N,N-dimethylformamide in two separated syringes. Fourier transform infrared spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy verified the existence of PVA/PEI and PLA in the fibrous membrane. We attempted to incorporate PEI with PLA as ultra-fine fibers to diminish the acidic inflammation caused by biodegradation of PLA. The fibrous composite membrane of PVA/PEI-PLA could provide better biocompatibility and would be used as drug-delivery carriers or tissue-engineering scaffolds.

  11. New locality record of Isomyia paurogonita Fang & Fan, 1986 (Diptera: Calliphoridae) from Peninsular Malaysia and Borneo.

    Science.gov (United States)

    Heo, C C; Aisha, S; Kurahashi, H; Omar, B

    2013-03-01

    Isomyia paurogonita Fang & Fan, 1986 (Diptera: Calliphoridae), a rare species of the subfamily Rhiniinae (tribe Cosminini) was recorded for the first time in Malaysia. We collected one male and two females during a field trip conducted at Genting Highland, Pahang, peninsular Malaysia in May 2011. A 3-day old cow liver was offered as attractant and dipterans collected were transferred to the laboratory for specimens processing and identification. The adults of I. paurogonita were attracted to the odour and then captured by using a sweep net. Isomyia paurogonita was also recorded from two other localities in Peninsular and Malaysian Borneo, namely Gombak Utara, Selangor and Sibu, Sarawak.

  12. San Marino.

    Science.gov (United States)

    1985-02-01

    San Marino, an independent republic located in north central Italy, in 1983 had a population of 22,206 growing at an annual rate of .9%. The literacy rate is 97% and the infant mortality rate is 9.6/1000. The terrain is mountainous and the climate is moderate. According to local tradition, San Marino was founded by a Christian stonecutter in the 4th century A.D. as a refuge against religious persecution. Its recorded history began in the 9th century, and it has survived assaults on its independence by the papacy, the Malatesta lords of Rimini, Cesare Borgia, Napoleon, and Mussolini. An 1862 treaty with the newly formed Kingdom of Italy has been periodically renewed and amended. The present government is an alliance between the socialists and communists. San Marino has had its own statutes and governmental institutions since the 11th century. Legislative authority at present is vested in a 60-member unicameral parliament. Executive authority is exercised by the 11-member Congress of State, the members of which head the various administrative departments of the goverment. The posts are divided among the parties which form the coalition government. Judicial authority is partly exercised by Italian magistrates in civil and criminal cases. San Marino's policies are tied to Italy's and political organizations and labor unions active in Italy are also active in San Marino. Since World War II, there has been intense rivalry between 2 political coalitions, the Popular Alliance composed of the Christian Democratic Party and the Independent Social Democratic Party, and the Liberty Committee, coalition of the Communist Party and the Socialist Party. San Marino's gross domestic product was $137 million and its per capita income was $6290 in 1980. The principal economic activities are farming and livestock raising, along with some light manufacturing. Foreign transactions are dominated by tourism. The government derives most of its revenue from the sale of postage stamps to

  13. Cellular delivery of shRNA using aptamer-conjugated PLL-alkyl-PEI nanoparticles.

    Science.gov (United States)

    Askarian, Saeedeh; Abnous, Khalil; Taghavi, Sahar; Oskuee, Reza Kazemi; Ramezani, Mohammad

    2015-12-01

    Introduction of an efficient gene delivery vector is still the main challenge of gene therapy. Both polyethylenimine (PEI) and poly(l-lysine) (PLL) comprise disadvantages which limited their application. To explore whether their deficiencies could be compensated by preparing copolymers consisting of both PLL and PEI, we generated several combinations of PLL-alkyl-PEI copolymers conjugated to aptamer and evaluated their both gene delivery efficiency and down-regulation of Bcl-XL, an anti-apoptotic gene, in lung cancer cell line. PLL was conjugated to either 10% or 50% of PEI by grafting different percentages of PEI to alkylated-PLL as core. The properties of modified polymers including size, surface charge density, DNA condensation ability, buffering capacity and cytotoxicity were evaluated. According to transfection results, aptamer conjugated PLL-alkyl-10%-PEI (PLPE8%) was selected for further gene silencing study by plasmid shRNA. Decrease in Bcl-XL gene expression was estimated by both RT-PCR and western-blot experiments. The obtained results revealed that the new copolymers had appropriate nano-scale size (117-128 nm) even after aptamer conjugation (168-183 nm). Moreover, they exhibited increased transfection efficiencies by up to 1.8-5 folds and acceptable cytotoxicity. The apoptosis was induced in transfected cells by shRNA-aptamer-copolymer due to the down-regulation of mRNA and protein levels. This study suggested a new vector for targeted non-viral gene delivery with high transfection efficiency in lung cancer or pulmonary systems. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. [Comparative study of theoretical literature on cold pathogenic disease in Wai tai mi yao fang (Arcane Essentials from the Imperial Library) and Tai ping sheng hui fang (Taiping Holy Prescriptions for Universal Relief)].

    Science.gov (United States)

    Zhang, Huirui; Liang, Yongxuan

    2014-09-01

    In the Wai tai mi yao fang (Arcane Essentials from the Imperial Library) compiled in 752, its portion on cold pathogenic disorders embodies the achievements before the mid Tang Dynasty, whereas that in the Tai ping sheng hui fang (Taiping Holy Prescriptions for Universal Relief), compiled in 992 embodies those before the early Song Dynasty. Comparison on the theory of cold disorders in both books reveal that, during the 2 centuries period from mid Tang to early Song Dynasties, the texts as a carrier for the transmission of such theory in both show no distinct changes, but only with minor revisions and improvements.

  15. Facile fabrication of AgNPs/(PVA/PEI) nanofibers: high electrochemical efficiency and durability for biosensors.

    Science.gov (United States)

    Zhu, Han; Du, MingLiang; Zhang, Ming; Wang, Pan; Bao, ShiYong; Wang, LiNa; Fu, YaQin; Yao, JuMing

    2013-11-15

    A novel, facile and green approach for the fabrication of H2O2, glutathione (GSH) and glucose detection biosensor using water-stable PVA and PVA/PEI nanofibers decorated with AgNPs by combining an in situ reduction approach and electrospinning technique has been demonstrated. Small, uniform and well-dispersed AgNPs embedded in the PVA nanofibers and immobilized on functionalized PVA/PEI nanofibers indicate the highly sensitive detection of H2O2 with a detection limit of 5 μM and exhibit a fast response, broad linear range, low detection limit and excellent stability and reusability.

  16. Jiang Tang Fang Long Jiao Nang in Treating Non-Insulin Dependent Diabetes Mellitus with Hearing Loss

    Institute of Scientific and Technical Information of China (English)

    LI Rui-yu; LI Meng; WANG Rui; LI Bin; GUO Ya-jing

    2008-01-01

    To study the efficacy and safety of the Jiang Tang Fang Long Jiao Nang (Hypoglycemic Anti-Deafness Capsule) in treating non-insulin dependent diabetes mellitus with hearing loss. Methods Two hundred ninety six patients with non-insulin dependent diabetes mellitus and hearing loss were randomly assigned to a treatment group(n=164, 208 ears) and a control group(n=132,184 ears). Patients in the treatment group were treated with the Jiang Tang Fang Long Jiao Nang and supplement herbal preparations as indicated by traditional Chinese medicine dialectical assessment, while control patients received glibenclamide and conventional treatments for deafness. Hearing, fasting blood glucose (FBG), post-prandial blood glucose(PBG), 24 hour urine sugar, platelet function indices, blood superoxide dismutase (SOD) and lipid peroxides (LPO) levels, and symptom improvement were compared between the two groups. Results The rate of hearing improvement was 56.7% for the treatment group and 26.6% for the control. FBG, PBG and 24 hour urine sugar improved in both groups, but the last two were superior in the treatment group compared to the control. Symptoms improvement was also superior in the treatment group compared to the control. In patients receiving Jiang Tang Fang Long Jiao Nang treatment, platelet function indices, SOD and LPO were all improved, while only LOP improvement was noticed in control patients. No acute or long-term toxicity was demonstrated for the Jiang Tang Fang Long Jiao Nang in animal tests. The Jiang Tang Fang Long Jiao Nang lowered blood glucose and serum triglycerides in a rat model of alloxan-induced diabetes. Conclusion The Jiang Tang Fang Long Jiao Nang is effective in improving hearing and diabetic indices in diabetic patients with deafness, without significant side effects.

  17. [Clinical effects of Fu Fang Ya Tong Ding on gingivitis and pericoronitis].

    Science.gov (United States)

    Wan, Hu-Chun; Zhang, Shuang; Wu, Ni-Ya; Kang, Dong; Hu, Li-Jie; Wang, Li-Zhi; Zhang, Ji-Ying; Xie, Ping

    2008-04-01

    To evaluate the clinical effects of Fu Fang Ya Tong Ding on treatment of gingivitis and pericoronitis. 120 clinical patients with gingivitis or pericoronitis were randomly divided into 3 groups (40 patients in each group). After routine rinse treatment for all patients, patients in the test group were treated with Fu Fang Ya Tong Ding, patients in the positive group were treated with iodine glycerol, while that time patients in the negative group received no treatment anymore. Ten minutes after treatment, visual analogue scale (VAS) was used to record the severity of pain for each patient. 3 days and 7 days later, pain and inflammation degree were also recorded by pain three-degree scoring method and index of gingivitis. The total treatment effects were evaluated under a comprehensive clinical treatment standard. 10 minutes after treatment, 40.0% of patients in the test group had almost no pain, while no obvious reduction of pain was found in the control group. 3 days, 7 days after the treatment, 92.5%, 95.0% of patients in the test group had no pain, and 55.0%, 90.0% of patients in the positive group had no pain. In the negative group, there were 47.5% of patients which pain was still remained in 7 days. 7 days after treatment, gingival index in the test group reduced by 25.0% and 42.8% compared with the positive and negative groups (Ppericoronitis through significantly reducing inflammation and pain.

  18. Two new species of fanged frogs from Peninsular Malaysia (Anura: Dicroglossidae).

    Science.gov (United States)

    Matsui, Masafumi; Belabut, Daicus M; Ahmad, Norhayati

    2014-11-03

    Taxonomic status of fanged frogs from the Peninsular Malaysia, previously assigned to Limnonectes kuhlii, is assessed using genetic and morphological approaches. Phylogenetic relationships inferred from sequences of the mitochondrial and nuclear genes revealed that the fanged frogs from the Peninsula form a monophyletic group and are clearly divergent from other species previously, or even now, assigned to L. kuhlii from Mainland Southeast Asia. In both mtDNA and nuDNA phylogeny, the Malay Peninsula clade diverges into two lineages, one from north (Larut Hill, Perak, and Hulu Terengganu, Terengganu) and another from south (Genting Highlands, Pahang, and Gombak, Selangor). These lineages are separated by large genetic distances, comparable with those observed between some other species of L. kuhlii-like frogs. Although the two lineages are very similar morphologically, they are distinguishable in several morphological traits and are considered heterospecific. We therefore describe them as L. utara sp. nov. and L. selatan sp. nov. These new species differ from all other species of kuhlii-like frogs from Mainland Southeast Asia by the surface of tibia, which is densely covered by large warts. 

  19. [An investigation into the Jin xiao fang (Prescriptions of Instant Effect), a lost recipe book of the Tang Dynasty].

    Science.gov (United States)

    Huang, Bin; Zhang, Yin-Zhu

    2013-07-01

    Altogether 141 prescriptions in the Jin xiao fang (Prescriptions of Instant Effect), a lost recipe book of the Tang Dynasty are cited in the Wai tai mi yao (Medical Secrets of An Official). Based on the analysis of these citations, it can be sure that the Jin xiao fang was written by Li Jian-yi of the Tang Dynasty, and completed around 724 to 733. These cited prescriptions are related to the clinical subjects, with complicate origins, for example, physicians and scholars. It is characterized by its "xiao yan (efficacy)".

  20. The Possible "Proton Sponge " Effect of Polyethylenimine (PEI) Does Not Include Change in Lysosomal pH

    DEFF Research Database (Denmark)

    Søndergaard, Rikke Vicki; Mattebjerg, Maria Ahlm; Henriksen, Jonas Rosager

    2013-01-01

    " hypothesis. Our measurements show that PEI does not induce change in lysosomal pH as previously suggested and quantification of PEI concentrations in lysosomes makes it uncertain that the "proton sponge " effect is the dominant mechanism of polyplex escape.Molecular Therapy (2012); doi:10.1038/mt.2012.185....

  1. PEG-PEI/siROCK2 Protects Against Aβ42-Induced Neurotoxicity in Primary Neuron Cells for Alzheimer Disease.

    Science.gov (United States)

    Liu, Yunyun; Yang, Xingyi; Lei, Qingfeng; Li, Zhong; Hu, Jingyang; Wen, Xiaojun; Wang, Huijun; Liu, Zhonglin

    2015-08-01

    Gene therapy that targets the ROCK2 gene has yielded promising results in the treatment of AD. Our previous study indicated that PEG-PEI/siROCK2 could effectively suppress ROCK2 mRNA expression and showed a promising prospect for the treatment of Alzheimer's disease. However, the ability of PEG-PEI/siROCK2 to reduce Aβ-induced cytotoxicity is unknown. To investigate the effect of PEG-PEI/siROCK2 against Aβ42-induced neurotoxicity, primary cultured cortical neurons were pretreated with PEG-PEI/siROCK2 for 24 h and then treated with 5 μM Aβ42 for 24 h. We found that PEG-PEI/siROCK2 increased the cell viability and reduced the number of apoptotic cells induced by Aβ42, as measured using an MTT assay and Annexin V/PI staining. A further study revealed that PEG-PEI/siROCK2 can activate p-Akt, and treatment with the PI3K inhibitor LY294002 attenuated the neuroprotective effects. These results suggest that PEG-PEI/siROCK2 prevents Aβ42-induced neurotoxicity and that the activation of PI3K/Akt pathway is involved in neuroprotection. Taken together, these findings shed light on the role of PEG-PEI/siROCK2 as a potential therapeutic agent for AD.

  2. Stabilization of Candida antarctica Lipase B (CALB Immobilized on Octyl Agarose by Treatment with Polyethyleneimine (PEI

    Directory of Open Access Journals (Sweden)

    Sara Peirce

    2016-06-01

    Full Text Available Lipase B from Candida antarctica (CALB was immobilized on octyl agarose (OC and physically modified with polyethyleneimine (PEI in order to confer a strong ion exchange character to the enzyme and thus enable the immobilization of other enzymes on its surface. The enzyme activity was fully maintained during the coating and the thermal stability was marginally improved. The enzyme release from the support by incubation in the non-ionic detergent Triton X-100 was more difficult after the PEI-coating, suggesting that some intermolecular physical crosslinking had occurred, making this desorption more difficult. Thermal stability was marginally improved, but the stability of the OCCALB-PEI was significantly better than that of OCCALB during inactivation in mixtures of aqueous buffer and organic cosolvents. SDS-PAGE analysis of the inactivated biocatalyst showed the OCCALB released some enzyme to the medium during inactivation, and this was partially prevented by coating with PEI. This effect was obtained without preventing the possibility of reuse of the support by incubation in 2% ionic detergents. That way, this modified CALB not only has a strong anion exchange nature, while maintaining the activity, but it also shows improved stability under diverse reaction conditions without affecting the reversibility of the immobilization.

  3. EFFECTS OF JIANG TANG FANG LONG FORMULA ON INSULIN PRODUCTION AND FUNCTION IN AN ANIMAL MODEL OF DIABETIC HEARING LOSS

    Institute of Scientific and Technical Information of China (English)

    YANG Yunxia; LI Ruiyu; JING Jianmei; ZHANG Yanzhuo; LI Meng; LI Bin; FENG Jinping; LI Li; LI Huizhen

    2013-01-01

    Objective To study the influence of Jiang Tang Fang Long formula on insulin production and function in an animal model of diabetic hearing loss. Methods Wistar rats (n=60) were randomly divided into 6 groups (10 in each) to receive no treatment (the normal control, Group A), or to receive intra-peritoneal 55 mg/kg streptozotocin with (Groups C, D and E) or without (Group B) subsequent Jiang Tang Fang Long formula treatment at various doses or Yu Long Wan treatment (Group F). After 60 days, fasting blood glucose (FBG), body weight (BW) and fasting insulin (FINS) were recorded and the HOMA-IR and HOMA-βcalcu-lated. Insulin expression in pancreatic tissues was measured by radioimmunoassay. Results Compared with animals that received streptozotocin without rescue treatment (Group B), animals that received higher doses of Jiang Tang Fang Long formula(Groups D and E) showed improved indices of diabetes manifestation (P0.05). Conclusion Our results suggest that Jiang Tang Fang Long formula may improve pancreatic β-cells function which may explain its efficacy in treating diabetic hearing loss.

  4. Anterior Kidney of the Yellow Mystus, Hemibagrus filamentus (Fang and Chaux, 1949

    Directory of Open Access Journals (Sweden)

    Sinlapachai SENARAT

    2013-12-01

    Full Text Available There is no available report on histological observation of the anterior kidney of the yellow mystus Hemibagrus filamentus (Fang and Chaux, 1949. Thus, the histology of the anterior kidney of specimens from the Tapee river, Chawang district, Nakhon Si Thammarat province, Thailand, was investigated under a light microscope in this study. The results revealed that the anterior kidney of H. filamentus consists of 3 distinct parts; pro, mid and hind anterior kidneys. Based upon different observations of the excretory portion and tissue types, this presented study is considered to be the first such report from Thailand. The pro-anterior kidney comprises of interregnal and exclusively hematopoietic tissues. The mid-anterior kidney is moderately composed of hematopoietic tissue and an increase of the melanomacrophage center (MMC with renal tubules. The last part of is the anterior kidney, which contains mainly renal tubules with a lesser amount of interstitial hematopoietic, which an increase in the glomerulus.

  5. Phylogeography of the fanged dicroglossine frog, Limnonectes fujianensis (Anura, Ranidae), in Taiwan.

    Science.gov (United States)

    Jang-Liaw, Nian-Hong; Chou, Wen-Hao

    2011-04-01

    A phylogenetic analysis of Taiwanese fanged dicroglossine frog, Limnonectes fujianensis (Anura, Ranidae), was conducted to examine its genetic diversification using sequence data from a portion of the mitochondrial DNA (mtDNA) cytochrome b sequences. We collected genetic data from 200 individuals at 23 localities in Taiwan and three localities in China. A neighbor-joining tree of 39 haplotypes revealed two clades in Taiwan and a clade in China, each showing restricted geographical distribution. The pattern of geographical divergence suggests a single invasion into Taiwan. Divergence times between clades were inferred using molecular clock tests. The population relationship of L. fujianensis between Taiwan and mainland China, and the phylogenetic relationships with its congeners, e.g., L. bannaensis, L. fragilis and L. kuhlii, were obtained and discussed.

  6. Receptor-mediated gene delivery using polyethylenimine (PEI)coupled with polypeptides targeting FGF receptors on cells surface

    Institute of Scientific and Technical Information of China (English)

    LI Da; WANG Qing-qing; TANG Gu-ping; HUANG Hong-liang; SHEN Fen-ping; LI Jing-zhong; YU Hai

    2006-01-01

    Objective: To construct a novel kind ofnonviral gene delivery vector based on polyethylenimine (PEI) conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods:The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results: The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR16 could be blocked by excess free polypeptides. Conclusion: The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy.

  7. Amiloidose renal em cão Shar-Pei: Relato de Caso Renal amyloidosis in a Shar-Pei dog: A case report

    Directory of Open Access Journals (Sweden)

    J.L. Reis Jr.

    2001-08-01

    Full Text Available O presente relato descreve os achados clínicos e anatomopatológicos de um caso de amiloidose renal em um cão macho de nove anos da raça Shar-Pei. O animal apresentava quadro clínico de esporotricose e de insuficiência renal e exames positivos para erlichiose e leishmaniose. No dia anterior ao óbito, o cão apresentou apatia, desidratação e anúria. À necropsia foram observados inúmeros pontos milimétricos esbranquiçados localizados no córtex renal e hepatização do lobo diafragmático esquerdo. O achado histológico mais importante foi deposição de material eosinofílico, amorfo e acelular localizado nos tufos glomerulares que se corou positivamente pelo vermelho congo (amilóide. Observaram-se nefrite supurada multifocal, espessamento da cápsula de Bowman e broncopneumonia supurada crônica, com fibrose intensa. A origem da amiloidose, no presente caso, poderia ser hereditária, assemelhando-se à amiloidose familiar descrita em cães da raça Shar-Pei, ou ser devida à inflamação supurada crônica e/ou leishmaniose.The clinical and pathological findings of a case of renal amyloidosis in a nine-year-old male Shar-Pei dog were described. Clinically, there were signs of sporotrichosis and renal insufficiency, besides being positive to leishmaniasis and ehrlichiosis. On the day before death, the animal became apathetic, dehydrated and anuric. On gross examination, there were several whitish millimetric spots seen widespread in both renal cortices and consolidation of the left diaphragmatic pulmonary lobe. The most important microscopic finding was a deposition of amorphous acellular material on the glomerular tufts which stained positively by congo red stain. Other changes were multifocal suppurative nephritis, thickening of the Bowman capsule and chronic suppurative bronchopneumonia. The origin of the amyloidosis in this case could be hereditary, being similar to familiar amyloidosis described in Shar-Pei breed, or due to

  8. Carboxymethylcellulose (CMC) formed nanogels with branched poly(ethyleneimine) (bPEI) for inhibition of cytotoxicity in human MSCs as a gene delivery vehicles.

    Science.gov (United States)

    Yang, Han Na; Park, Ji Sun; Jeon, Su Yeon; Park, Keun-Hong

    2015-05-20

    Specific vehicles are necessary for safe and efficient gene transfection into cells. Nano-type hydrogels (nanogel) comprising carboxymethylcellulose (CMC) complexed with branched type cationic poly(ethleneimine) (bPEI) were used as gene delivery vehicles. When complexes of CMC and bPEI were used in vitro, CMC showed nano-gel type properties, as shown by the results of a viscosity test, and bPEI showed low cytotoxicity comparing to bPEI alone. Together, these properties are shown to maintain high gene transfection efficiency. In viability experiments using three types of adult stem cells, cell viability varied depending on the branch form of PEI and whether or not it is in a complex with CMC. The gene delivery efficacy showed that the CMC nanogel complexed with bPEI (CMC-bPEI) showed more uptaking and gene transfection ability in hMSCs comparing to bPEI alone. In osteogenesis, the CMC-bPEI complexed with OSX pDNA showed more easy internalization than bPEI alone complexed with OSX pDNA in hMSCs. Specific genes and proteins related in osteogenic differentiation were expressed in hMSCs when the CMC-bPEI complexed with OSX pDNA was used.

  9. Optimization of Adsorption Conditions of Cr (VI by PEI Modified BSG Using Response Surface Methodology

    Directory of Open Access Journals (Sweden)

    Xinlong Jiang

    2015-02-01

    Full Text Available Surface response optimization of Adsorption Conditions of Cr (VI wastewater by PEI modified brewer's grains (BSG with the factors of pH value, adsorbent concentration, adsorption time, amount of adsorbent and the response of adsorption rate were studied. The optimal parameters for adsorption conditions were of adsorbent concentration of 113.30 mg/L, adsorbent particle size of 60~80 mesh, pH 1.79, adsorbent amount of 4.99 g/L, adsorption time and temperature of 1.88 h and 30°C, respectively. The maximal absorption rate got 100.0%, adsorption capacity was 46.58 mg/g. The PEI modified BSG is a promising, cheap, efficient, new biological materials of adsorption for Cr (VI in wastewater.

  10. Synthesis Technology of PEI Modified Brewer's Grains for the Adsorption of Cr(VI

    Directory of Open Access Journals (Sweden)

    Yihua Jiang

    2015-05-01

    Full Text Available With beer production waste brewer's grains (BSG as the raw material, through polyethylenimine (PEI modified and glutaraldehyde (GA cross-linking process for preparing the modified brewer's grains biosorbents. The simulation of Cr (VI wastewater as treatment object, through the optimization of synthesis conditions of Cr (VI, the optimal synthesis process was obtained. The results showed, 4 g BSG and 100 mL 4% PEI methanol solution were placed in 250 mL conical flask at room temperature, shaking for 24 h. After the mixture,shifted to 200 mL l.5% GA solution directly, magnetic stirring for half an hour, the modified results of brewer’s grain was best. Under the optimal conditions, the average of adsorption rate was 98.82% and adsorption capacity was 45.31 mg/g. The modified brewer's grains of amine surfactant modified is a promising treatment of chromium wastewater biological materials.

  11. Back pain and body posture evaluation instrument (BackPEI): development, content validation and reproducibility.

    Science.gov (United States)

    Noll, Matias; Tarragô Candotti, Cláudia; Vieira, Adriane; Fagundes Loss, Jefferson

    2013-08-01

    Following a search conducted in several databases, no instrument was found that jointly evaluates the prevalence of back pain and its associated demographic, social-economic, hereditary, behavioral and postural risk factors. Thus, the present study aims to develop the Back Pain and Body Posture Evaluation Instrument (BackPEI) for school-age children and verify its validity and reproducibility. Twenty-one questions were elaborated to compose the BackPEI instrument, eight experts checked the content validity, and its reproducibility was tested by applying the questionnaire to 260 primary schoolchildren, at two different times with a 7-day interval. The reproducibility data for the first 20 questions, analyzed using the kappa (k) coefficient, were classified as "very good" (k > 0.8) or "good" (0.6 back pain and its associated risk factors.

  12. STRUCTURE AND THERMAL MECHANICAL PROPERTIES OF ORIENTED POLYIMIDE (PEI-E)

    Institute of Scientific and Technical Information of China (English)

    SUN Zhenhua; ZHUANG Yugang; LI Shichun; DING Mengxian; FENG Zhiliu

    1992-01-01

    The strain induced crystallization behaviour in polyimide from 1 , 4-bis (3 ', 4 '- dicarboxyphenoxy)benzene and 4,4'-oxydianiline (PEI-E)has been investigated by WAXD, DSC and FTIR. The results obtained show that crystallization in PEI-E did take place just after tensile yielding. Meanwhile, the effect of strain induced crystallization on the thermomechanical properties was studied by DMA and TMA, the results of which indicate that the crystallization and hot stretching have a certain influence on the dynamic mechanical properties, such as weakening the β relaxation and decreasing the glass transition temperature. The TMA results confirm the shifting of glass transition temperature to lower temperature region after hot stretching. This phenomenon could be well explained by the effect of residual stress according to Eyring's theory.

  13. Resistance welding of carbon fibre reinforced polyetheretherketone composites using metal mesh and PEI film

    Institute of Scientific and Technical Information of China (English)

    闫久春; 王晓林; 秦明; 赵新英; 杨士勤

    2004-01-01

    Weldability of polyetheretherketone(PEEK) with polyetherimide(PEI) is tested. And carbon fiber reinforced PEEK laminates are resistance welded using stainless steel mesh heating element. The effects of the welding time and welding pressure on the lap shear strength of joints are investigated. Results show that PEEK can heal with PEI well in welding condition and the lap shear strength of PEEK/CF(carbon fibre) joint increases linearly with welding time, but reaches a maximum value when welding pressure ranging from 0.3MPa to 0.5MPa with constant welding time. The fracture characteristics of surface are analyzed by SEM techniques, and four types of fracture modes of lap shear joints are suggested.

  14. Henry Moore’s Public Sculpture in the US: The Collaborations with I. M. Pei

    Directory of Open Access Journals (Sweden)

    Alex Potts

    2016-07-01

    Full Text Available The many commissions Henry Moore received for public sculpture in the United States provided the occasion for several quite distinctive works. While not site specific, these were unique, and their final form, scale, and disposition was elaborated with a particular setting in mind. This aspect of Moore’s work in the US, which began with the monumental piece he designed for the Lincoln Center in New York in 1963–65, is examined here by focusing on the productive relationship he forged with the architect I. M. Pei in the 1970s. The sculptures Moore produced in collaboration with Pei respond in suggestive ways to the spatial environments created in American cities by late modern architectural developments. They also realize an oddly effective combination of the biomorphic and abstract that differs both from the bodily conception of Moore’s earlier work and the non-figurative character of much public sculpture of the time.

  15. Algae harvesting for biofuel production: influences of UV irradiation and polyethylenimine (PEI) coating on bacterial biocoagulation.

    Science.gov (United States)

    Agbakpe, Michael; Ge, Shijian; Zhang, Wen; Zhang, Xuezhi; Kobylarz, Patricia

    2014-08-01

    There is a pressing need to develop efficient and sustainable separation technologies to harvest algae for biofuel production. In this work, two bacterial species (Escherichia coli and Rhodococus sp.) were used as biocoagulants to harvest Chlorella zofingiensis and Scenedesmus dimorphus. The influences of UV irradiation and polyethylenimine (PEI)-coating on the algal harvesting efficiency were investigated. Results showed that the UV irradiation could slightly enhance bacteria-algae biocoagulation and algal harvesting efficiency. In contrast, the PEI-coated E. coli cells noticeably increased the harvesting efficiencies from 23% to 83% for S. dimorphus when compared to uncoated E. coli cells. Based on the soft-particle Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, an energy barrier existed between uncoated E. coli cells and algal cells, whereas the PEI coating on E. coli cells eliminated the energy barrier, thereby the biocoagulation was significantly improved. Overall, this work presented groundwork toward the potential use of bacterial biomass for algal harvesting from water. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. Polyethyleneimine (PEI mediated siRNA gene silencing in the Schistosoma mansoni snail host, Biomphalaria glabrata.

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    Matty Knight

    2011-07-01

    Full Text Available An in vivo, non-invasive technique for gene silencing by RNA interference (RNAi in the snail, Biomphalaria glabrata, has been developed using cationic polymer polyethyleneimine (PEI mediated delivery of long double-stranded (ds and small interfering (si RNA. Cellular delivery was evaluated and optimized by using a 'mock' fluorescent siRNA. Subsequently, we used the method to suppress expression of Cathepsin B (CathB with either the corresponding siRNA or dsRNA of this transcript. In addition, the knockdown of peroxiredoxin (Prx at both RNA and protein levels was achieved with the PEI-mediated soaking method. B. glabrata is an important snail host for the transmission of the parasitic digenean platyhelminth, Schistosoma mansoni that causes schistosomiasis in the neotropics. Progress is being made to realize the genome sequence of the snail and to uncover gene expression profiles and cellular pathways that enable the snail to either prevent or sustain an infection. Using PEI complexes, a convenient soaking method has been developed, enabling functional gene knockdown studies with either dsRNA or siRNA. The protocol developed offers a first whole organism method for host-parasite gene function studies needed to identify key mechanisms required for parasite development in the snail host, which ultimately are needed as points for disrupting this parasite mediated disease.

  17. Preparation and characterization of PEG-PEI/Fe3O4 nano-magnetic fluid by co-precipitation method

    Institute of Scientific and Technical Information of China (English)

    PENG Jian; ZOU Fen; LIU Lu; TANG Liang; YU Li; CHEN Wei; LIU Hui; TANG Jing-bo; WU Li-xiang

    2008-01-01

    PEG-PEI/Fe3O4 nano-magnetic fluids with different mass fractions of reactant were prepared by co-precipitation method. Besides particle size analyzer, the methods of XRD, IR, VSM and AFM were adopted to characterize the synthesized samples. Covalent bonding of PEG, PEI and Fe3O4 exhibits superparamagnetism. The TEM photograph shows that the particles are of stable dispersion and little aggregation, with smooth surface, spherical shape and a diameter of about 80 nm, which meets the requirements of nano-materials. When the mass fraction of PEI in reactant is 25%, the particle size, Zeta-potential and pEGFP-C1 DNA loading efficiency are all satisfactory. In this case, PEG-PEI/ Fe3O4 nano-magnetic fluids can be used as gene vectors or targeted drug carriers.

  18. Studies on preparing and adsorption property of grafting terpolymer microbeads of PEI-GMA/AM/MBA for bilirubin.

    Science.gov (United States)

    Gao, Baojiao; Lei, Haibo; Jiang, Liding; Zhu, Yong

    2007-06-15

    Crosslinking copolymer microbeads with a diameter range of 100-150 microm were synthesized by suspension copolymerization of glycidyl methacrylate (GMA), acrylamide (AM) and N,N'-methylene bisacrylamide (MBA). Subsequently, polyethyleneimine (PEI) was grafted on the surfaces of the terpolymer microbeads GMA/AM/MBA via the ring-opening reaction of the epoxy groups, and the grafting microbeads PEI-GMA/AM/MBA were prepared. In this paper, the adsorption property of the grafting microbeads for bilirubin was mainly investigated, and the effects of various factors, such as pH value, ionic strength and grafting degree of PEI on the surface of grafting microbeads and the adsorption capacity of the grafting microbeads for bilirubin were examined. The batch adsorption experiment results show that by right of the action of grafted polyamine macromolecules PEI, the grafting microbeads PEI-GMA/AM/MBA have quite strong adsorption ability for bilirubin; the isotherm adsorption conforms to Freundlich equation. The pH value of the medium affects the adsorption capacity greatly, As in the nearly neutral solutions with pH 6, the grafting microbeads have the strongest adsorption ability for bilirubin, whereas in acidic and basic solutions their adsorption ability is weak. The ionic strength hardly affects the adsorption ability of the grafting microbeads. The grafting degree of PEI on the surfaces of the grafting microbeads also has a great effect on the adsorption capacity, and higher the grafting degree of PEI on the surface of the microbead PEI-GMA/AM/MBA, the stronger is the adsorption ability of the microbeads.

  19. Bioreducible PEI-siRNA Nanocomplex for Liver Cancer Therapy: Transfection, Biodistribution, and Tumor Growth Inhibition In Vivo

    Directory of Open Access Journals (Sweden)

    Wei Xia

    2013-01-01

    Full Text Available A bioreducible polyethylenimine (SS-PEI was successfully applied as a nonviral carrier for the delivery of plasmid DNA and VEGF-siRNA in vitro and in vivo. The SS-PEI could strongly condense DNA or siRNA into nanosized complexes (below 200 nm with positive surface charges. In vitro transfection experiments using GFP plasmid as gene reporter showed that the complexes of SS-PEI/DNA were able to efficiently transfect HepG2 cells, with efficiency comparable to that of polyethylenimine, a gold standard for nonviral gene delivery. Moreover, the complexes of SS-PEI/VEGF-siRNA could lead to reduced levels of VEGF protein in HepG2 cells in vitro. Treatment with the complexes of SS-PEI/VEGF-siRNA efficiently inhibited HepG2 tumor growth in an xenograft mouse model. The data of this study imply that the SS-PEI is a potent nucleic acid carrier applicable for liver cancer gene therapy.

  20. Erwinia amylovora novel plasmid pEI70: complete sequence, biogeography, and role in aggressiveness in the fire blight phytopathogen.

    Directory of Open Access Journals (Sweden)

    Pablo Llop

    Full Text Available Comparative genomics of several strains of Erwinia amylovora, a plant pathogenic bacterium causal agent of fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised of plasmids. We recently identified and sequenced in full a novel 65.8 kb plasmid, called pEI70. Annotation revealed a lack of known virulence-related genes, but found evidence for a unique integrative conjugative element related to that of other plant and human pathogens. Comparative analyses using BLASTN showed that pEI70 is almost entirely included in plasmid pEB102 from E. billingiae, an epiphytic Erwinia of pome fruits, with sequence identities superior to 98%. A duplex PCR assay was developed to survey the prevalence of plasmid pEI70 and also that of pEA29, which had previously been described in several E. amylovora strains. Plasmid pEI70 was found widely dispersed across Europe with frequencies of 5-92%, but it was absent in E. amylovora analyzed populations from outside of Europe. Restriction analysis and hybridization demonstrated that this plasmid was identical in at least 13 strains. Curing E. amylovora strains of pEI70 reduced their aggressiveness on pear, and introducing pEI70 into low-aggressiveness strains lacking this plasmid increased symptoms development in this host. Discovery of this novel plasmid offers new insights into the biogeography, evolution and virulence determinants in E. amylovora.

  1. Erwinia amylovora novel plasmid pEI70: complete sequence, biogeography, and role in aggressiveness in the fire blight phytopathogen.

    Science.gov (United States)

    Llop, Pablo; Cabrefiga, Jordi; Smits, Theo H M; Dreo, Tanja; Barbé, Silvia; Pulawska, Joanna; Bultreys, Alain; Blom, Jochen; Duffy, Brion; Montesinos, Emilio; López, María M

    2011-01-01

    Comparative genomics of several strains of Erwinia amylovora, a plant pathogenic bacterium causal agent of fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised of plasmids. We recently identified and sequenced in full a novel 65.8 kb plasmid, called pEI70. Annotation revealed a lack of known virulence-related genes, but found evidence for a unique integrative conjugative element related to that of other plant and human pathogens. Comparative analyses using BLASTN showed that pEI70 is almost entirely included in plasmid pEB102 from E. billingiae, an epiphytic Erwinia of pome fruits, with sequence identities superior to 98%. A duplex PCR assay was developed to survey the prevalence of plasmid pEI70 and also that of pEA29, which had previously been described in several E. amylovora strains. Plasmid pEI70 was found widely dispersed across Europe with frequencies of 5-92%, but it was absent in E. amylovora analyzed populations from outside of Europe. Restriction analysis and hybridization demonstrated that this plasmid was identical in at least 13 strains. Curing E. amylovora strains of pEI70 reduced their aggressiveness on pear, and introducing pEI70 into low-aggressiveness strains lacking this plasmid increased symptoms development in this host. Discovery of this novel plasmid offers new insights into the biogeography, evolution and virulence determinants in E. amylovora.

  2. Morphological characterization of nanocomposites of membrane PEI/regional clay; Caracterizacao morfologica de membranas de nanocompositos de PEI/argila regional

    Energy Technology Data Exchange (ETDEWEB)

    Medeiros, Vanessa da N.; Leite, Amanda M.D.; Paz, Rene A. da; Araujo, Edcleide M., E-mail: vanismedeiros@gmail.com [Dept. de Engenharia de Materiais, Universidade Federal de Campina Grande - UFCG, Campina Grande, PB (Brazil); Pessan, Luiz A. [Universidade Federal de Sao Carlos - UFSCar, Sao Carlos, SP (Brazil)

    2011-07-01

    The interest in the nanotechnology development in the last decade allows the increase in the nanocomposite area due to the special properties presented by these materials. In the nanocomposite, the nanometric particles are dispersed in the polymer matrix. The membrane separation process, although is recently, is increasing in applications such as purification, fractioning and concentration of substances in several industries. The membranes to gas separation present specific advantages when compared with conventional separation process (such as, cryogenic distillation and adsorption). The aim of this work is to prepare plane membrane by evaporation-precipitation method. These membranes were obtained from polyetherimide (PEI) with bentonite clay from Paraiba/Brazil. The results showed that by XRD, it can be observed there is not practically intercalation of polymer molecules between the layers of clay and was seen by SEM that the membranes have dense structures, ie, favorable for application in gas separation. (author)

  3. 羟基喜树碱/GA-PEI-PLGA纳米粒的制备及其工艺优化%Preparation and Optimization of Hydroxycamptothecin Loaded GA-PEI-PLGA Nanoparticles

    Institute of Scientific and Technical Information of China (English)

    梁劲康; 吴得天; 胡巧红; 吴雅琼

    2016-01-01

    目的:制备包载羟基喜树碱(HCPT)的甘草次酸修饰的 PEI-PLGA(GA-PEI-PLGA)纳米粒。方法采用超声乳化-减压溶剂挥发法制备 HCPT/ GA-PEI-PLGA 纳米粒。在单因素实验基础上,选取药物/载体比、油相/水相体积比、超声时间、超声功率为考察因素,以包封率和载药量为考察指标,通过正交设计实验对载药纳米粒的制备工艺进行优化,并对其粒径及 Zeta 电位进行测定。结果超声乳化-减压溶剂挥发法成功制得 HCPT/ GA-PEI-PLGA 纳米粒,其中药物/载体比对载药纳米粒制备的影响最大。优化后的处方工艺为:药物/载体比3:10、油相/水相体积比1:9、超声功率570 W、超声时间15 min。制得的载药纳米粒包封率高达(87.52±3.91)%,载药量为(20.10±4.72)%;平均粒径(218.1±3.23)nm,Zeta 电位为(34.98±3.56)mV。结论超声乳化-减压溶剂挥发法适于制备 HCPT/ GA-PEI-PLGA 纳米粒,制得的载药纳米粒包封率和载药量较高、粒径分布均匀、稳定性好。%Objective To prepare hydroxycamptothecin(HCPT)loaded glycyrrhetinic acid modified polyethyleneimine-poly(lactic-co-glycolic acid)( GA-PEI-PLGA)nanoparticles. Methods Emulsion-solvent evaporation method was used to prepare HCPT-loaded GA-PEI-PLGA nanoparticles. Based on the single-factor experiments,drug/ GA-PEI-PLGA ratio,oil phase/ water phase ratio,sonication timeand power were further investigated. The formulation and process were optimized by orthogonal testingdesign using entrapment efficiency and drug loading efficiency as indexes. HCPT-loaded GA-PEI-PLGA nanoparticles ˊ diameter and zeta potential were determined. Results HCPT-loaded GA-PEI-PLGA nanoparticles were successfully prepared by emulsion-solvent evaporation method.Drug/ GA-PEI-PLGA ratio,in particular,had the uniform particle on the preparation of HCPT-loaded nanoparticles.The optimal process was as follows:drug/ GA-PEI-PLGA ratio of

  4. [Textual research on circulation of the Ming edition of Li Heng's Xiu zhen fang (Pocket Formulary)].

    Science.gov (United States)

    Yang, Jinping; Liu, Peng; Lu, Mingjing; Lu, Xing; Li, Shaolin; Jin, Xiumei

    2015-03-01

    Xiu zhen fang (Pocket Formulary) is a recipe book of the Ming Dynasty, inspired and managed by Zhu Su, compiled by Li Heng of liangyisuo (good physician house) in Zhou wangfu (Zhou's royal palace). The book was compiled and published twice during the reigns of the Hongwu and Yongle Emperors of the Ming Dynasty. Because of its high practicability, there were some editions in circulation, and the book was published several times only in the Ming Dynasty. At present, the earliest extanteditionwas the little character version of Yongle, and the version in the 4(th) year of Zhengde Emperor of the Ming Dynasty was a reprinting edition based on the Yongle edition, sharingthe same edition system. Most of the editions appeared after the reign of Zhengtong Emperor of the Ming Dynasty, titled by "kui ben (head version)" and "da quan (complete edition)" were the editionspublished in the local bookshops, which had rather distinct differences from the Yongle edition system not only in the its format but also in its contents.

  5. Histology and Immunohistochemistry Study on Mianbu Fang Healing Immunological Infertility of Male Mice

    Institute of Scientific and Technical Information of China (English)

    王望九; 黄震; 汤明礼; 李笑梅; 陈永华; 产美英; 胡德宝; 戴宁; 孔梅

    2000-01-01

    Objective To observe the histological and immunohistochemical changes caused by of Traditional Chinese Medicine——Mianbu Fang (Immunological Infertility Healing Mixture) in testis and epididymus of male mice with immunological infertility induced by antisperm antibody (AsAb ).Materials & Methods Models of mice with immunological infertility were used by in-jection of mice sperm. These mice were treated with Mianbu Ⅰ , Mianbu Ⅱ or Pred-nisone Acetates. The characteristics on histology and immunohistochemistry of mice were analyzed.Results High level of AsAb was detected in serum and seminal vesicle plasma of model mice after treatment. Immune compounds mostly deposited in limiting membrane of seminiferous tubules, spermatogonium and epithelia of epididymis ducts. The num-ber of spermatogenic cells per convoluted seminiferous tubule decreased. However, the Chinese medicine could significantly reduce the level of AsAb, or even eliminate the an-tibodies and clear out the immune compounds and increase the number of spermatogenic cells of semini ferous tubules.Conclusion Chinese Medicine Mianbu Ⅰ and Ⅱ can regulate, absorb and clear out cir-culation and partial AsAb, as well as immunological compound. Hence, they can in-crease the number of spermatogenic cells of semini ferous tubules and increase the preg-nancy of mice.

  6. Histology and Immunohistochemistry Study on Mianbu Fang Healing Immunological Infertility of Male Mice

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective To observe the histological and immunohistochemical changes caused by of Traditional Chinese Medicine--Mianbu Fang (Immunological Infertility Healing Mixture) in testis and epididymus of male mice with immunological infertility induced by antisperm antibody (AsAb ). Materials & Methods Models of mice with immunological infertility were used by in jection of mice sperm. These mice were treated with Mianbu Ⅰ , Mianbu Ⅱ or Pred nisone Acetates. The characteristics on histology and immunohistochemistry of mice were analyzed. Results High level of AsAb was detected in serum and seminal vesicle plasma of model mice after treatment. Immune compounds mostly deposited in limiting membrane of seminiferous tubules, spermatogonium and epithelia of epididymis ducts. The num ber of spermatogenic cells per convoluted seminiferous tubule decreased. However, the Chinese medicine could significantly reduce the level of As Ab, or even eliminate the an tibodies and clear out the immune compounds and increase the number of spermatogenic cells of seminiferous tubules. Conclusion Chinese Medicine Mianbu Ⅰ and Ⅱ can regulate, absorb and clear out cir culation and partial AsAb, as well as immunological compound. Hence, they can in crease the number of spermatogenic cells of seminiferous tubules and increase the preg nancy of mice.

  7. Preparation of PEI/PVA Superfine Fiber Loaded with Nano-Rhodium Hybrid Material by Electrospinning Technique%静电纺丝制备PEI/PVA超细纤维负载纳米铑杂化材料的研究

    Institute of Scientific and Technical Information of China (English)

    蔡盛赢; 蔡颖婷; 朱罕; 王盼; 包世勇; 邹美玲; 杜明亮; 张明

    2013-01-01

    利用NaBH4作为还原剂,表没食子儿茶素没食子酸酯(EGCG)为稳定剂,还原RhCl3水溶液制备铑纳米颗粒,并用透射电镜(TEM)研究了铑纳米颗粒的形貌.通过静电纺丝技术制备了PEI/PVA纳米纤维,并用戊二醛作为交联剂使之交联以提高其耐水性.然后在交联过的PEI/PVA纤维膜上负载铑纳米颗粒;利用傅里叶红外光谱仪(FTIR)、扫描电子显微镜(SEM)对PEI/PVA以及Rh/PEI/PVA薄膜进行了表征.结果表明:利用静电纺丝技术和复合技术可以制备PEI/PVA超细纤维负载纳米铑的杂化材料.

  8. Studies on Heteroses of Pei'ai 64S/Javanica Rice

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Agronomic traits and heteroses of javanica varieties and the indica/javanica hybrids in Changsha are reported. Javanica rice exhibits long panicles, big grains, less panicles per plant, a long growth duration and high plant height in Changsha. The hybrid of Pei'ai 64S/javanica possesses positive heterobeltiosis in culm height, panicle length, panicles per plant, spikelets per panicle, grains per panicle, yield per plant, theoretic yield per hectare and actual yield per hectare; performs negative heterobeltiosis in days to flower, seed setting rate and 1000-grain weight. There are significant linear regression relations between culm height of the hybrid and panicle length of its male parent, panicle length of the hybrid and that of its male parent, spikelets per panicle of the hybrid and spikelets per panicle and panicle length of its male parent, grains per panicle of the hybrid and spikelets per panicle of its male parent, 1000-grain weight of the hybrid and spikelets per panicle of its male parent, respectively. In general, significant differences at 1% level are seen in most traits except grains per panicle and theoretic yield per hectare between the indica/javanica hybrid and Pei'ai 64S/Teqin (CK). Compared with the check, the indica/javanica hybrids have positive standard heterosis in culm height, panicle length, spikelets per panicle, grains per panicle and 1000-grain weight but negative standard heterosis in days to flower, panicles per plant, seed setting rate, theoretic yield per hectare and actual yield per hectare. For the individuals, 11.1% hybrids possess > 40% standard heterosis in theoretic yield per hectare, 3.7% possess >40% standard heterosis in actual yield per hectare. The javanica restorer line 770 derives from a single cross of Pei'ai 64S/Moroberekan B-51-4-9-2. The hybrid 43S/770 possesses an elite plant type and strong heterosis.

  9. Hyaluronic acid reagent functional chitosan-PEI conjugate with AQP2-siRNA suppressed endometriotic lesion formation

    Directory of Open Access Journals (Sweden)

    Zhao MD

    2016-03-01

    Full Text Available Meng-Dan Zhao,1 Jin-Lin Cheng,2 Jing-Jing Yan,1 Feng-Ying Chen,1 Jian-Zhong Sheng,3 Dong-Li Sun,1 Jian Chen,4 Jing Miao,4 Run-Ju Zhang,1 Cai-Hong Zheng,1 He-Feng Huang1,5 1Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, 2State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, 3Department of Pathophysiology, School of Medicine, 4The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 5International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China Abstract: To identify a new drug candidate for treating endometriosis which has fewer side effects, a new polymeric nanoparticle gene delivery system consisting of polyethylenimine-grafted chitosan oligosaccharide (CSO-PEI with hyaluronic acid (HA and small interfering RNA (siRNA was designed. There was no obvious difference in sizes observed between (CSO-PEI/siRNAHA and CSO-PEI/siRNA, but the fluorescence accumulation in the endometriotic lesion was more significant for (CSO-PEI/siRNAHA compared with CSO-PEI/siRNA due to the specific binding of HA to CD44. In addition, the (CSO-PEI/siRNAHA nanoparticle gene therapy significantly decreased the endometriotic lesion sizes with atrophy and degeneration of the ectopic endometrium. The epithelial cells of ectopic endometrium from rat models of endometriosis showed a significantly lower CD44 expression than control after treatment with (CSO-PEI/siRNAHA. Furthermore, observation under an electron microscope showed no obvious toxic effect on the reproductive organs. Therefore, (CSO-PEI/siRNAHA gene delivery system can be used as an effective method for the treatment of endometriosis. Keywords: chitosan-PEI, hyaluronic acid, AQP2-siRNA, endometriosis, targeted therapy 

  10. The protective effect of Liu-Wei-Di-Huang-Fang in salt-sensitive hypertension rats.

    Science.gov (United States)

    Yang, Qiang; He, Yanming; Wang, Wenjian

    2014-01-01

    Abstract Hypertension is considered as a chronic and complex disease relating to multiple systemic systems. Apart from lowering blood pressure, the final purpose of the treatment lies in reducing the variability of blood pressure and other risk factors. Traditional Chinese medicine (TCM) has a long history of treating hypertension. This study was designed to determine the effect of Liu-Wei-Di-Huang-Fang (L-W-D-H-F), a compound used in traditional Chinese herbal medicine, to treat salt-sensitive hypertension (SSHT) induced by a high-salt and high-fat diet. L-W-D-H-F was prepared from six plant extracts. It was dissolved in 0.9% sodium chloride solution prior to use. Male Sprague-Dawley (6 weeks) rats were randomly divided into four groups: normal diet (CON); HSF (Without Drug Intervention); VAL (Valsartan 13.33 mg/kg/day); and LW (L-W-D-H-F 8.13 g/kg/day). Six weeks after blood pressure treatment, plasma biochemical analyses and histological and functional examination of the kidney were performed. L-W-D-H-F decreased the levels of mean arterial pressure (MAP), fasting blood glucose (FG), insulin (INS), high-density lipoprotein cholesterol (HDL-c), homeostasis model assessment of basal insulin resistance (HOMA-IR) and angiotensin II (Ang II) from plasma and Ang II and renin from kidney. It also promoted the excretion of urinary Na(+), reducing the loss of urinary K(+) and microalbuminuria (MAU), and improved the glomerular afferent arteriole, arterioles and each kidney unit. Together, these results suggest that L-W-D-H-F is capable of moderately reducing MAP in salt-sensitive hypertension and can work at different levels on multiple differential targets.

  11. Enhanced Delivery of Plasmid Encoding Interleukin-12 Gene by Diethylene Triamine Penta-Acetic Acid (DTPA)-Conjugated PEI Nanoparticles.

    Science.gov (United States)

    Dehshahri, Ali; Sadeghpour, Hossein; Keykhaee, Maryam; Khalvati, Bahman; Sheikhsaran, Fatemeh

    2016-05-01

    Recombinant therapeutic proteins have been considered as an efficient category of medications used for the treatment of various diseases. Despite their effectiveness, there are some reports on the systemic adverse effects of recombinant therapeutic proteins limiting their wide clinical applications. Among different cytokines used for cancer immunotherapy, interleukin-12 (IL-12) has shown great ability as a powerful antitumor and antiangiogenic agent. However, significant toxic reactions following the systemic administration of IL-12 have led researchers to seek for alternative approaches such as the delivery and local expression of the IL-12 gene inside the tumor tissues. In order to transfer the plasmid encoding IL-12 gene, the most extensively investigated polycationic polymer, polyethylenimine (PEI), was modified by diethylene triamine penta-acetic acid (DTPA) to modulate the hydrophobic-hydrophilic balance of the polymer as well as its toxicity. DTPA-conjugated PEI derivatives were able to form complexes in the size range around 100-180 nm with great condensation ability and protection of the plasmid against enzymatic degradation. The highest gene transfer ability was achieved by the DTPA-conjugated PEI at the conjugation degree of 0.1 % where the level of IL-12 production increased up to twofold compared with that of the unmodified PEI. Results of the present study demonstrated that modulation of the surface positive charge of PEI along with the improvement of the polymer hydrophobic balance could be considered as a successful strategy to develop safe and powerful nanocarriers.

  12. Enhanced CO2 adsorptive performance of PEI/SBA-15 adsorbent using phosphate ester based surfactants as additives.

    Science.gov (United States)

    Cheng, Dandan; Liu, Yue; Wang, Haiqiang; Weng, Xiaole; Wu, Zhongbiao

    2015-12-01

    In this study, a series of polyetherimide/SBA-15: 2-D hexagonal P6mm, Santa Barbara USA (PEI/SBA-15) adsorbents modified by phosphoric ester based surfactants (including tri(2-ethylhexyl) phosphate (TEP), bis(2-ethylhexyl) phosphate (BEP) and trimethyl phosphonoacetate (TMPA)) were prepared for CO2 adsorption. Experimental results indicated that the addition of TEP and BEP had positive effects on CO2 adsorption capacity over PEI/SBA-15. In particular, the CO2 adsorption amount could be improved by around 20% for 45PEI-5TEP/SBA-15 compared to the additive-free adsorbent. This could be attributed to the decrease of CO2 diffusion resistance in the PEI bulk network due to the interactions between TEP and loaded PEI molecules, which was further confirmed by adsorption kinetics results. In addition, it was also found that the cyclic performance of the TEP-modified adsorbent was better than the surfactant-free one. This could be due to two main reasons, based on the results of in situ DRIFT and TG-DSC tests. First and more importantly, adsorbed CO2 species could be desorbed more rapidly over TEP-modified adsorbent during the thermal desorption process. Furthermore, the enhanced thermal stability after TEP addition ensured lower degradation of amine groups during adsorption/desorption cycles.

  13. Enhanced corrosion protective PANI-PAA/PEI multilayer composite coatings for 316SS by spin coating technique

    Energy Technology Data Exchange (ETDEWEB)

    Syed, Junaid Ali; Lu, Hongbin; Tang, Shaochun; Meng, Xiangkang, E-mail: mengxk@nju.edu.cn

    2015-01-15

    Highlights: • PANI-PAA/PEI multilayers with controllable thickness were fabricated by spin assembly. • PAA matrix results in the homogeneous dispersion of PANI in the composite coatings. • Spin coating combined with heating assures the linear increase in thickness with n. • The corrosion protection property of PANI-PAA/PEI coatings were optimized at n = 20. • Enhanced protection owing to multilayer structure that lengthens the diffusion pathway of ions. - Abstract: In the present study, polyaniline-polyacrylic acid/polyethyleneimine (PANI-PAA/PEI) composite coatings with a multilayer structure for corrosion protection of 316 stainless steels (316SS) were prepared by an alternate deposition. Spin coating combined with heating assists removal of residual water that result in a linear increase in thickness with layer number (n). The combination of PANI-PAA composite with PEI and their multilayer structure provides a synergistic enhancement of corrosion resistance properties as determined by electrochemical measurements in 3.5% NaCl solution. Importantly, the PANI-PAA/PEI coating with an optimized layer number of n = 20 shows improved corrosion protection. The superior performance was attributed to the formation of an interfacial oxide layer as well as the multilayer structure that extend the diffusion pathway of corrosive ions.

  14. Novel PEI/Poly-γ-Gutamic Acid Nanoparticles for High Efficient siRNA and Plasmid DNA Co-Delivery

    Directory of Open Access Journals (Sweden)

    Shu-Fen Peng

    2017-01-01

    Full Text Available The efficient delivery of sufficient amounts of nucleic acids into target cells is critical for successful gene therapy and gene knockdown. The DNA/siRNA co-delivery system has been considered a promising approach for cancer therapy to simultaneously express and inhibit tumor suppressor genes and overexpressed oncogenes, respectively, triggering synergistic anti-cancer effects. Polyethylenimine (PEI has been identified as an efficient non-viral vector for transgene expression. In this study, we created a very high efficient DNA/siRNA co-delivery system by incorporating a negatively-charged poly-γ-glutamic acid (γ-PGA into PEI/nucleic acid complexes. Spherical nanoparticles with about 200 nm diameter were formed by mixing PEI/plasmid DNA/siRNA/γ-PGA (dual delivery nanoparticles; DDNPs with specific ratio (N/P/C ratio and the particles present positive surface charge under all manufacturing conditions. The gel retardation assay shows both nucleic acids were effectively condensed by PEI, even at low N/P ratios. The PEI-based DDNPs reveal excellent DNA/siRNA transfection efficiency in the human hepatoma cell line (Hep 3B by simultaneously providing high transgene expression efficiency and high siRNA silencing effect. The results indicated that DDNP can be an effective tool for gene therapy against hepatoma.

  15. Novel PEI/Poly-γ-Gutamic Acid Nanoparticles for High Efficient siRNA and Plasmid DNA Co-Delivery.

    Science.gov (United States)

    Peng, Shu-Fen; Hsu, Hung-Kun; Lin, Chun-Cheng; Cheng, Ya-Ming; Hsu, Kuang-Hsing

    2017-01-04

    The efficient delivery of sufficient amounts of nucleic acids into target cells is critical for successful gene therapy and gene knockdown. The DNA/siRNA co-delivery system has been considered a promising approach for cancer therapy to simultaneously express and inhibit tumor suppressor genes and overexpressed oncogenes, respectively, triggering synergistic anti-cancer effects. Polyethylenimine (PEI) has been identified as an efficient non-viral vector for transgene expression. In this study, we created a very high efficient DNA/siRNA co-delivery system by incorporating a negatively-charged poly-γ-glutamic acid (γ-PGA) into PEI/nucleic acid complexes. Spherical nanoparticles with about 200 nm diameter were formed by mixing PEI/plasmid DNA/siRNA/γ-PGA (dual delivery nanoparticles; DDNPs) with specific ratio (N/P/C ratio) and the particles present positive surface charge under all manufacturing conditions. The gel retardation assay shows both nucleic acids were effectively condensed by PEI, even at low N/P ratios. The PEI-based DDNPs reveal excellent DNA/siRNA transfection efficiency in the human hepatoma cell line (Hep 3B) by simultaneously providing high transgene expression efficiency and high siRNA silencing effect. The results indicated that DDNP can be an effective tool for gene therapy against hepatoma.

  16. Stylistic Awareness and Historical Writing to City Image in Novels by Fang Fang%论方方长篇小说的文体意识与城市形象的历史书写

    Institute of Scientific and Technical Information of China (English)

    肖敏

    2015-01-01

    Style is that of text with language as carrier ,which reflects the spirit mechanism and ways of thinking of the writer. In short ,it means the writer ’s discourse style and a series of content behind. In her writ⁃ing recently ,Fang Fang has shown a certain stylistic awareness ,with a sense of city culture. Fang Fang took special narrative perspective and narrative structure to achieve the purpose of writing the history and culture of Wuhan ,with different style from others in language. When she transform her creation ,trying to be more skill⁃ful for novels ,Wuhan city gave her rich ideological and cultural resources;Fang Fang less throughout took the non-focus perspective ,but prone to adopt the point-to-area novel structure ,to achieve the effect of being graceful easy. In language ,she was not reliant on the local language means to achieve the purpose of creation , nor stopped at the general writing of folkways in Wuhan ,but trying to show us the deep cultural character of Wuhan by means of her unique artistic language.%文体是以语言为载体的文本体式,也反映了作家本人之精神机制与思维方式。简言之,文体表现为作家的话语体式及其背后的一系列内容。方方近年来的长篇小说创作,体现出了一定的文体意识,具有城市文化感。方方采取特殊叙事视角和叙事结构等策略来达到对武汉历史文化的书写目的,在语言上也有不同于一般汉派作家的文体特征。在方方实现其创作转型、试图更娴熟地掌握长篇小说这种形式时,武汉这座城市给予了她丰富的思想资源和文化资源;方方较少通篇采用非聚焦视角,倾向采用以点带面的小说结构,以达到疏朗有致的效果。在语言方面,方方并不通过倚重还原本地语言的手段来达到创作目的,也没有止步于一般性地书写武汉的市民性特征,而是试图用其独特的语言手段和艺术方式来挖掘武汉的深层文化品格。

  17. 77 FR 54811 - Safety Zone; TriRock San Diego, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2012-09-06

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; TriRock San Diego, San Diego Bay, San Diego... safety zone upon the navigable waters of the San Diego Bay, San Diego, CA, in support of a bay swim in San Diego Harbor. This safety zone is necessary to provide for the safety of the participants, crew...

  18. 78 FR 58878 - Safety Zone; San Diego Shark Fest Swim; San Diego Bay, San Diego, CA

    Science.gov (United States)

    2013-09-25

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego Shark Fest Swim; San Diego Bay, San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone upon the navigable waters of the San Diego Bay, San Diego, CA, in support of San...

  19. 78 FR 53243 - Safety Zone; TriRock San Diego, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2013-08-29

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; TriRock San Diego, San Diego Bay, San Diego... temporary safety zone upon the navigable waters of the San Diego Bay, San Diego, CA, in support of a... Bryan Gollogly, Waterways Management, U.S. Coast Guard Sector San Diego; telephone (619) 278-7656, email...

  20. PEI Domestic Oil Tank Program : a history of recent oil spills on Prince Edward Island

    Energy Technology Data Exchange (ETDEWEB)

    McInnis, D.

    2001-04-01

    This power point presentation addressed the issue of recent oil spills on Prince Edward Island (PEI) with a critical examination of the Home Heat Tank Program in the province and how insurance companies, banks and real estate industry are dealing with the problem of oil spill contamination. The number of above ground domestic home heating tank spills continues to remain high and have been increasing in the past 10 years. This is due in part to improper installations which are essentially putting the safety of many homeowners and the environment at risk. It was noted that in Prince Edward Island, home heat tank installations do not have to be performed by qualified persons. Therefore, in order to save money, many home owners cut corners. Most improper installations are attributed to people who are not aware of proper guidelines and do not follow proper requirements and guidelines. In 1999 there were 208 domestic oil spills reported in PEI, of which 125 involved 17 tons or less of contaminated soil, 62 involved 51 tons of contaminated soil and 21 involved 51 tons or more of contaminated soil. The cost of cleanup for the latter was in the tens of thousands of dollars. It was recommended that this problem should be addressed in a 4 phase approach involving stakeholder consultation, public education, program assessment and regulation amendments. 37 figs.

  1. Combination of adenovirus and cross-linked low molecular weight PEI improves efficiency of gene transduction

    Energy Technology Data Exchange (ETDEWEB)

    Han Jianfeng; Zhao Dong; Zhong Zhirong; Zhang Zhirong; Gong Tao; Sun Xun, E-mail: xunsun22@gmail.com [Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, 610041 (China)

    2010-03-12

    Recombinant adenovirus (Ad)-mediated gene therapy is an exciting novel strategy in cancer treatment. However, poor infection efficiency with coxsackievirus and adenovirus receptor (CAR) down-regulated cancer cell lines is one of the major challenges for its practical and extensive application. As an alternative method of viral gene delivery, a non-viral carrier using cationic materials could compensate for the limitation of adenovirus. In our study, adenovectors were complexed with a new synthetic polymer PEI-DEG-bis-NPC (PDN) based on polyethylenimine (PEI), and then the properties of the vehicle were characterized by measurement of size distribution, zeta potential and transmission electron microscopy (TEM). Enhancement of gene transduction by Ad/PDN complexes was observed in both CAR-overexpressing cell lines (A549) and CAR-lacking cell lines (MDCK, CHO, LLC), as a result of facilitating binding and cell uptake of adenoviral particles by the cationic component. Ad/PDN complexes also promoted the inhibition of tumor growth in vivo and prolonged the survival time of tumor-bearing mice. These data suggest that a combination of viral and non-viral gene delivery methods may offer a new approach to successful cancer gene therapy.

  2. Use of PEI-coated Magnetic Iron Oxide Nanoparticles as Gene Vectors

    Institute of Scientific and Technical Information of China (English)

    韦卫中; 徐春芳; 吴华

    2004-01-01

    To evaluate the feasibility of using polyethyleneimine (PEI) coated magnetic iron oxide nanoparticles (polyMAG-1000) as gene vectors. The surface characteristics of the nanoparticles were observed with scanning electron microscopy. The ability of the nanoparticles to combine with and protect DNA was investigated at different PH values after polyMAG-1000 and DNA were combined in different ratios. The nanoparticles were tested as gene vectors with in vitro transfection models. Under the scanning electron microscope the nanoparticles were about 100 nm in diameter.The nanoparticles could bind and condense DNA under acid, neutral and alkaline conditions, and they could transfer genes into cells and express green fluorescent proteins (GFP). The transfection efficiency was highest (51 %) when the ratio of nanoparticles to DNA was 1:1 (v:w). In that ratio, the difference in transfection efficiency was marked depending on whether a magnetic field was present or not: about 10 % when it was absent but 51 % when it was present. The magnetic iron oxide nanoparticles coated with PEI may potentially be used as gene vectors.

  3. Utilization of PEI-modified Corynebacterium glutamicum biomass for the recovery of Pd(II) in hydrochloric solution.

    Science.gov (United States)

    Won, Sung Wook; Park, Jiyeong; Mao, Juan; Yun, Yeoung-Sang

    2011-02-01

    A new type of biosorbent was developed for binding anionic precious metals through cross-linking waste biomass Corynebacterium glutamicum with polyethylenimine (PEI). This biomass was evaluated for the removal and recovery of palladium and compared to commercial adsorbents, such as Amberjet 4200 Cl, Lewatit Monoplus TP 214, SPC-100, and SPS-200. The kinetic experiments revealed that the sorption equilibrium was reached with 30 min for the PEI-modified biomass. The maximum uptake of the biosorbent was 176.8 mg/g, which was calculated using the Langmuir model. The Pd(II) maximum uptake exhibited the following order: Amberjet 4200 Cl>Lewatit Monoplus TP 214>PEI-modified biomass>SPC-100>SPS-200. Acidified thiourea in 1.0M HCl was used to desorb Pd(II) from all of the sorbents examined.

  4. Targeted delivery of Bcl-2 conversion gene by MPEG-PCL-PEI-FA cationic copolymer to combat therapeutic resistant cancer.

    Science.gov (United States)

    Li, Zibiao; Liu, Xuan; Chen, Xiaohong; Chua, Ming Xuan; Wu, Yun-Long

    2017-07-01

    Deregulation of anti-apoptosis Bcl-2 protein expression was a key feature in human cancers with therapeutic resistance. Nuclear receptor Nur77 could induce the conformation change of Bcl-2 protein and converted it into an apoptosis inducer by "enemy to friend" strategy. However, the safe and effective delivery of this gene to combat therapeutic resistant cancer remained largely unexplored. In this report, we designed an amphiphilic cationic MPEG-PCL-PEI-FA copolymer, comprising biocompatible and hydrophilic methoxy-poly(ethylene glycol) (MPEG), biodegradable and hydrophobic poly(ε-caprolactone) (PCL), cationic poly(ethylene imine) (PEI) segments, and folic acid (FA) as targeting group, as a high efficient Nur77 gene carrier to folate receptor (FR) highly expressed and therapeutic resistant HeLa/Bcl-2 cancer cells. Interestingly, due to the incorporation of PCL and PEG segments, this MPEG-PCL-PEI-FA copolymer showed less toxicity but better gene transfection efficiency than non-viral gene carrier gold standard PEI (25kDa). This might be due to the formation of micelles to stabilize polyplex for enhanced gene transfection ability. More importantly, MPEG-PCL-PEI-FA copolymer exhibited excellent growth inhibition ability on therapeutic resistant HeLa/Bcl-2 cancer cells, which was FR overexpressed HeLa cervical cancer cells with high expression of Bcl-2 protein, thanks to its FA induced targeting ability, high gene transfection efficiency, and low cytotoxicity. This work signifies the first time that cationic amphiphilic MPEG-PCL-PEI-FA copolymers could be utilized for the gene delivery to therapeutic resistant cancer cells with high expression of anti-apoptosis Bcl-2 protein and the positive results are encouraging for the further design of polymeric platforms for combating drug resistant tumors. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Les noves tecnologies en el tractament i la valorització de fangs. Informe de síntesi

    OpenAIRE

    Obis, Josep M.; Moragas, L.; Moliner, Marc; Chornet, Esteban; Elías Castells, Xavier; Mata-Álvarez, Joan; Llorens, Joan; Torres, Ricard; Martín, Maria; Torrens, Esther; Stüber, Frank; Fortuny Sanromá, Agustín; Fabregat Llagostera, Azael; Bengoa, Christophe José; Font, Josep

    2007-01-01

    Aquesta reunió de treball (workshop) es realitzà com una activitat complementària de les III Jornades Tècniques, amb l’objectiu d’abordar les noves tecnologies en el tractament i la valorització de fangs i disposar d’informació de primera mà de les iniciatives de recerca, desenvolupament i innovació tecnològica en aquestes matèries. Més explícitament, l’objectiu ha consistit en aprofundir en les noves tecnologies i les seves possibilitats, analitzant no únicament les tecnologie...

  6. Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes.

    Science.gov (United States)

    Höbel, Sabrina; Loos, Andrea; Appelhans, Dietmar; Schwarz, Simona; Seidel, Jürgen; Voit, Brigitte; Aigner, Achim

    2011-01-20

    Polycationic non-viral polymers are widely employed as delivery platforms of plasmid DNA, or of small interfering RNAs (siRNAs) for the induction of RNA interference (RNAi). Among those, poly(ethylene imine)s (PEIs) take a prominent position due to their relatively high efficacy; however, their biodistribution profiles upon systemic delivery and their toxicity pose limitations which can be addressed by the introduction of PEI modifications. In this paper, we systematically analyse physicochemical and biological properties of DNA and siRNA complexes prepared from a set of maltose-, maltotriose- or maltoheptaose-modified hyperbranched PEIs (termed (oligo-)maltose-modified PEIs; OM-PEIs). We show that pH-dependent charge densities of the OM-PEIs correlate with the structure and degree of grafting, and the length of the oligomaltose. Decreased zeta potentials of OM-PEI-based complexes and changes in the thermodynamics of DNA complex formation are observed, while the complex sizes are largely unaffected by maltose grafting and the presence of serum proteins. Furthermore, although complexation efficacies of siRNAs are not altered, complex stabilities are markedly increased in OM-PEI complexes. DNA complex uptake and transfection kinetics are slowed down upon maltose-grafting of the PEI which can be attributed to decreased zeta potentials, and alterations in the uptake mechanisms (clathrin-dependent/clathrin-independent endocytosis) are observed. Independent of the maltose architecture, DNA and siRNA complexes based on maltose-grafted PEI show considerably lower cytotoxicity as compared to PEI complexes. While maltose grafting generally leads to reduced in vitro transfection efficacies, this effect is less profound in some OM-PEI/siRNA complexes as compared to OM-PEI/DNA complexes. Importantly, upon their systemic application in vivo, OM-PEI/siRNA complexes show marked differences in the siRNA biodistribution profile with e.g. substantially decreased siRNA levels in the

  7. The PEI-introduced CS shell/PMMA core nanoparticle for silencing the expression of E6/E7 oncogenes in human cervical cells.

    Science.gov (United States)

    Saengkrit, Nattika; Sanitrum, Phakorn; Woramongkolchai, Noppawan; Saesoo, Somsak; Pimpha, Nuttaporn; Chaleawlert-Umpon, Saowaluk; Tencomnao, Tewin; Puttipipatkhachorn, Satit

    2012-10-15

    In this study, we examined the potential of cationic nanoparticle - polyethyleneimine-introduced chitosan shell/poly (methyl methacrylate) core nanoparticles (CS-PEI) for siRNA delivery. Initially, DNA delivery was performed to validate the capability of CS-PEI for gene delivery in the human cervical cancer cell line, SiHa. siRNA delivery were subsequently carried out to evaluate the silencing effect on targeted E6 and E7 oncogenes. Physicochemical properties including size, zeta potential and morphology of CS-PEI/DNA and CS-PEI/siRNA complexes, were analyzed. The surface charges and sizes of the complexes were observed at different N/P ratios. The hydrodynamic sizes of the CS-PEI/DNA and CS-PEI/siRNA were approximately 300-400 and 400-500nm, respectively. Complexes were positively charged depending on the amount of added CS-PEI. AFM images revealed the mono-dispersed and spherical shapes of the complexes. Gel retardation assay confirmed that CS-PEI nanoparticles completely formed complexes with DNA and siRNA at a N/P ratio of 1.6. For DNA transfection, CS-PEI provided the highest transfection result. Localization of siRNA delivered through CS-PEI was confirmed by differential interference contrast (DIC) confocal imaging. The silencing effect of siRNA specific to HPV 16 E6/E7 oncogene was examined at 18 and 24h post-transfection. The results demonstrated the capacity of CS-PEI to suppress the expression of HVP oncogenes. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Aminopolymer Mobility and Support Interactions in Silica-PEI Composites for CO2 Capture Applications: A Quasielastic Neutron Scattering Study.

    Science.gov (United States)

    Holewinski, Adam; Sakwa-Novak, Miles A; Carrillo, Jan-Michael Y; Potter, Matthew E; Ellebracht, Nathan; Rother, Gernot; Sumpter, Bobby G; Jones, Christopher W

    2017-07-13

    Composite gas sorbents, formed from an active polymer phase and a porous support, are promising materials for the separation of acid gases from a variety of gas streams. Significant changes in sorption performance (capacity, rate, stability etc.) can be achieved by tuning the properties of the polymer and the nature of interactions between polymer and support. Here we utilize quasielastic neutron scattering (QENS) and coarse-grained molecular dynamics (MD) simulations to characterize the dynamic behavior of the most commonly reported polymer in such materials, poly(ethylenimine) (PEI), both in bulk form and when supported in a mesoporous silica framework. The polymer chain dynamics (rotational and translational diffusion) are characterized using two neutron backscattering spectrometers that have overlapping time scales, ranging from picoseconds to a few nanoseconds. Two modes of motion are detected for the PEI molecule in QENS. At low energy transfers, a "slow process" on the time scale of ∼200 ps is found and attributed to jump-mediated, center-of-mass diffusion. A second, "fast process" at ∼20 ps scale is also found and is attributed to a locally confined, jump-diffusion. Characteristic data (time scale and spectral weight) of these processes are compared to those characterized by MD, and reasonable agreement is found. For the nanopore-confined PEI, we observe a significant reduction in the time scale of polymer motion as compared to the bulk. The impacts of silica surface functionalization and of polymer fill fraction in the silica pores (controlling the portion of polymer molecules in contact with the pore walls), are both studied in detail. Hydrophobic functionalization of the silica leads to an increase of the PEI mobility above that in native silanol-terminated silica, but the dynamics are still slower than those in bulk PEI. Sorbents with faster PEI dynamics are also found to be more efficient for CO2 capture, possibly because sorption sites are more

  9. PEG-b-PPS-b-PEI micelles and PEG-b-PPS/PEG-b-PPS-b-PEI mixed micelles as non-viral vectors for plasmid DNA: tumor immunotoxicity in B16F10 melanoma.

    Science.gov (United States)

    Velluto, Diana; Thomas, Susan N; Simeoni, Eleonora; Swartz, Melody A; Hubbell, Jeffrey A

    2011-12-01

    Cationic micelles formed from poly(ethylene glycol)-bl-poly(propylene sulfide)-bl-poly(ethylene imine) (PEG-b-PPS-b-PEI) and from mixtures of poly(ethylene glycol)-bl-poly(propylene sulfide) (PEG-b-PPS) with PEG-b-PPS-b-PEI were explored as non-viral vectors for plasmid DNA (pDNA) transfection in a tumor immunotoxicity model. Complexes with pDNA were found to be templated exclusively by the size of the pDNA-free micelles and ranged from 240 nm (for PEG-b-PPS-b-PEI) to 30 nm (for mixed micelles of PEG-b-PPS/PEG-b-PPS-b-PEI). Both formulations transfected melanoma cells well in vitro. As a model with a functional read-out of tumor cell death, one with likely only small bystander effects, tumors were transfected with an antigen transgene, using an antigen to which the recipient animals had been previously vaccinated with a Th1-biasing adjuvant. Reduction in tumor growth, increase in intratumoral infiltration of cytotoxic T lymphocytes and accumulation of Th1-biasing cytokines indicated that both micelle formulations transfected efficiently compared with naked pDNA and with low cytotoxicity.

  10. 方英文小小说的智慧与情趣%Intelligence and Aesthetics in Fang Yingwen's Novelette

    Institute of Scientific and Technical Information of China (English)

    芦芙荭

    2014-01-01

    押中国当代著名作家方英文创作了不少精品小小说,博雅温情、幽默俊逸的作品风格,一直受到广大读者的热捧。方英文小小说,运用“失衡”的人物关系、“不常规”的视角等艺术手法,形成构思新颖、结尾惊人的艺术结构;在叙述上,借助第一人称、“段子”及独创的比喻等语言表现艺术,形成幽默中不失智慧的语言风格。%Fang Yingwen, Chinese celebrated contemporary writer, has created many boutique novelette, his erudition and warmth, humor and chaste writing style have been welcomed by the general readers. Fang's novelette used "imbalanced" character relationship and "unusual" artistic technique to form the art structure of novel design and amazing end; on the narrative, he used language art of first person, " skit " and original metaphor to form the humor and intelligent language style.

  11. 姜黄素-甘草次酸-PEI-PLGA 纳米粒的制备及工艺优化%Preparation of curcumin-glycyrrhetinic acid-PEI-PLGA nanoparticles and the process optimization

    Institute of Scientific and Technical Information of China (English)

    胡慧中; 郑晓君; 胡巧红

    2015-01-01

    Objective To prepare curcumin (Cur)-glycyrrhetinic acid (GA)-PEI-PLGA nanoparticles and optimize the preparation process .Methods Cur-GA-PEI-PLGA nanoparticles were prepared by thin film hydration method .After establishing the analysis method , single factor experiment and orthogonal design experiment were used to evaluate the effects of hydration temperature , hydration time and drug addition on the entrapment efficiency and drug loading , and to determine the optimum preparation conditions .Cur-GA-PEI-PLGA nanoparticles were prepared under the optimum preparation condi-tions , and we determined the entrapment efficiency and drug loading .The morphology of the nanoparticles was observed by transmission electron microscope (TEM).The diameter and zeta potential of the nanoparticles were determined by dynamic light scattering particle size analyzer ( DLS) .Results The optimum preparation conditions of Cur-GA-PEI-PLGA nanopar-ticles were as follows:the hydration temperature was 60 ℃, the hydration time was 3 h, and the drug addition was 7 mg. The entrapment efficiency and drug loading were (58.1 ±1.2)%and (14.5 ±1.4)%under these conditions .The nanop-articles were spherical and uniform under TEM .The average particle size was 330.3 nm, and the nanoparticles distributed evenly (PDI was 0.311), zeta potential was 39.2 mV.Conclusions Thin film hydration method is suitable for the prepa-ration of Cur-GA-PEI-PLGA nanoparticles .The optimum preparation conditions are as follows:the hydration temperature is 60 ℃, the hydration time is 3 h, and the drug addition is 7 mg.%目的:制备姜黄素(Cur)-甘草次酸(GA)-PEI-PLGA纳米粒,并优化其制备工艺。方法采用薄膜水化法制备Cur-GA-PEI-PLGA纳米粒,建立分析方法后,采用单因素和正交设计试验考察水化温度、水化时间、投药量对纳米粒包封率和载药量的影响,确定最佳制备条件。在最佳条件下制备Cur-GA-PEI-PLGA纳米粒,检测其包

  12. Alginate/PEI/DNA polyplexes: a new gene delivery system%藻酸盐/PEI/DNA复合载体作为一种新型基因递送系统

    Institute of Scientific and Technical Information of China (English)

    蒋革; 文相贤; 金美羅; 李东哲; 林美正; 廉荣一

    2006-01-01

    目的克服多聚乙烯亚胺(PEI,polyethlenimine)/DNA载体对细胞的毒性以及在含血清培养基里对癌细胞基因的转移率低的问题.方法利用具有水溶性、可生物降解的、并带有负电的藻酸盐(alginate)对PEI/DNA载体进行包衣,制备出复合载体,并在体外含50%血清培养基里,与PEI/DNA载体比较对C3癌细胞转染率.结果在含50%血清的培养基里,藻酸盐包衣制备的复合体载体[alginate:DNA,0.15(w/w);PEI:DNA,N:P=10]与PEI/DNA载体相比,对C3癌细胞基因转染率高出10~30倍,而且其表面正电荷数比PEI/DNA载体减少了一半,颗粒较小,并降低对细胞毒性和红血球集聚反应.结论作为新型的藻酸盐包衣制备的复合载体能提高在体外含高浓度血清培养基里对C3癌细胞的转染率,并能减少其对细胞毒性.%Aim To avoid the limitation of the use of cationic polyethlenimine (PEI)-complexed plasmid DNA use for in vitro or in vivo gene delivery due to its cytotoxicity and lower efficiency in the presence of serum. Methods A polyplex with decreased positive charge on the complex surface was designed. The PEI/DNA (PD) complexes coated with an anionic biodegradable polymer, alginate were prepared and their gene delivery behavior with PD was compared. Results The alginate-coated PD polyplex, where alginate: PEI: DNA [alginate: DNA, 0. 15 (w/w); PEI: DNA, N: P = 10] showed about 10 -30 fold-increased transfection efficiency compared to corresponding non-coated complexes to C3 cells in the presence of 50% serum. The surface charge of the alginate-coated complex was approximately half of that of the alginate-lacking complex. The size of alginate-coated complex was slightly smaller than that of the corresponding complex without alginate. The former complex also showed a reduced erythrocyte aggregation activity and decreased cytotoxicities to C3 cells in comparison with PD complex. Conclusion The alginate-coated PD polyplexes as a new gene delivery

  13. 《类聚方》与《金匮要略》对比研究%Comparison Study on Leiju Fang and Jinkui Yaolue

    Institute of Scientific and Technical Information of China (English)

    姚佳音

    2012-01-01

    This thesis refers to Japonic medical composing Leiju Fang , and contrasts Lei with the final edition of Jingui Yaolue in Song dynasty. It is indicated that Jingui Yaolue and Leiju Fang have diffences on content, interpunc-tion, prescription, dose, etc. The thesis provide the reference proof to the Jingui Yaolue related research.%参考日本医书《类聚方》,比照宋定本《金匮要略》,从文字,句读,方名,剂量等方面进行对比分析,为研究《金匮要略》提供参考依据.

  14. Comparison of PEI-PEG and PLL-PEG copolymer coatings on the prevention of protein fouling.

    Science.gov (United States)

    Bergstrand, Anna; Rahmani-Monfared, Ghazal; Ostlund, Asa; Nydén, Magnus; Holmberg, Krister

    2009-03-01

    The effect of surface charge on the protein resistance of adsorbed layers of poly(ethylene imine)-[g]-poly(ethylene glycol), PEI-PEG, and poly(L-lysine)-[g]-poly(ethylene glycol), PLL-PEG, was studied. Mixed and monofunctional self-assembled monolayers, SAMs, on gold were obtained by adsorption of 16-mercapto-1-hexadecanoic acid and 16-mercapto-1-hexadecanol. The surface charge was systematically varied by changing the ratio of the two alkanethiols. The graft copolymers PEI-PEG and PLL-PEG were adsorbed at the SAMs and tested for resistance towards human serum albumin and fibrinogen. The adsorbed amount of copolymers increased with increasing negative surface charge. However, the best protein resistance was found at an intermediate surface charge. The PLL-PEG covered surfaces showed better protein resistance than the PEI-PEG covered surfaces. Thus, this work demonstrates that an adsorbed layer of PEG-grafted PEI and, in particular, PEG-grafted PLL is efficient in preventing protein adsorption when there is charge neutralization between the copolymer and the underlying surface. (c) 2008 Wiley Periodicals, Inc.

  15. 76 FR 22724 - Draft Program Environmental Impact Statement/Environmental Impact Report (PEIS/R) and Public...

    Science.gov (United States)

    2011-04-22

    ...) to reduce or avoid adverse water supply impacts to all of the Friant Division long-term contractors... alternatives considered in the Draft PEIS/R include actions that will be implemented to work towards achieving... contracts between the United States and the Central Valley Project (CVP) Friant Division contractors....

  16. Effect of polymer concentration on the structure and performance of PEI hollow fiber membrane contactor for CO2 stripping.

    Science.gov (United States)

    Naim, R; Ismail, A F

    2013-04-15

    A series of polyetherimide (PEI) hollow fiber membranes with various polymer concentrations (13-16 wt.%) for CO2 stripping process in membrane contactor application was fabricated via wet phase inversion method. The PEI membranes were characterized in terms of liquid entry pressure, contact angle, gas permeation and morphology analysis. CO2 stripping performance was investigated via membrane contactor system in a stainless steel module with aqueous diethanolamine as liquid absorbent. The hollow fiber membranes showed decreasing patterns in gas permeation, contact angle, mean pore size and effective surface porosity with increasing polymer concentration. On the contrary, wetting pressure of PEI membranes has enhanced significantly with polymer concentration. Various polymer concentrations have different effects on the CO2 stripping flux in which membrane with 14 wt.% polymer concentration showed the highest stripping flux of 2.7 × 10(-2)mol/m(2)s. From the performance comparison with other commercial membrane, it is anticipated that the PEI membrane has a good prospect in CO2 stripping via membrane contactor.

  17. Coupling of a bifunctional peptide R13 to OTMCS-PEI copolymer as a gene vector increases transfection efficiency and tumor targeting

    Directory of Open Access Journals (Sweden)

    Lv H

    2014-03-01

    Full Text Available Hui Lv,1,* Qing Zhu,1,* Kewu Liu,2 Manman Zhu,1 Wenfang Zhao,1 Yuan Mao,1 Kehai Liu1 1Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai, People's Republic of China; 2Heilongjiang Forest By-Product and Speciality Institute, Mudanjiang, People's Republic of China *These authors contributed equally to this work Background: A degradable polyethylenimine (PEI derivative coupled to a bifunctional peptide R13 was developed to solve the transfection efficiency versus cytotoxicity and tumor-targeting problems of PEI when used as a gene vector. Methods: We crossed-linked low molecular weight PEI with N-octyl-N-quaternary chitosan (OTMCS to synthesize a degradable PEI derivative (OTMCS-PEI, and then used a bifunctional peptide, RGDC-Tat (49–57 called R13 to modify OTMCS-PEI so as to prepare a new gene vector, OTMCS-PEI-R13. This new gene vector was characterized by various physicochemical methods. Its cytotoxicity and gene transfection efficiency were also determined both in vitro and in vivo. Results: The vector showed controlled degradation and excellent buffering capacity. The particle size of the OTMCS-PEI-R13/DNA complexes was around 150–250 nm and the zeta potential ranged from 10 mV to 30 mV. The polymer could protect plasmid DNA from being digested by DNase I at a concentration of 23.5 U DNase I/µg DNA. Further, the polymer was resistant to dissociation induced by 50% fetal bovine serum and 400 µg/mL sodium heparin. Compared with PEI 25 kDa, the OTMCS-PEI-R13/DNA complexes showed higher transfection efficiency both in vitro and in vivo. Further, compared with OTMCS-PEI, distribution of OTMCS-PEI-R13 at tumor sites was markedly enhanced, indicating the tumor-targeting specificity of R13. Conclusion: OTMCS-PEI-R13 could be a potential candidate as a safe and efficient gene delivery carrier for gene therapy. Keywords: nonviral gene vector, polyethylenimine, R13, transfection efficiency

  18. 聚四氟乙烯和石墨对聚醚酰亚胺摩擦学性能的影响%Tribological Properties of PTFE/PEI and Graphite/PEI Blends

    Institute of Scientific and Technical Information of China (English)

    马彦琼; 谭忠阳; 李玉杰; 王大明; 党国栋; 陈春海

    2014-01-01

    The tribological performances of polyetherimide( PEI) composite materials filled with polytetraflu-oroethylene( PTFE) and graphite were evaluated on UMT-2 friction and wear tester, respectively. The micro-structures of worn surfaces, cross-sections of the PEI composite were examined with scanning electron micro-scope( SEM) , and wear mechanism was analyzed. The result showed that PEI composite filled with PTFE and graphite were improved significantly on friction and wear properties. The friction coefficient is reduced to below 0. 3(the friction coefficient of pure PEI is 0. 41), the wear rate is reduced by 3 orders of magnitude. When filled with 10%PTFE in composites PTFE system, the friction coefficient of the composites is lowest, and the friction coefficient of the graphite counterpart is lowest in 15% graphite content. The lowest friction coefficient is 0. 23 and 0. 27. For the wear rate, PEI composite materials filled with PTFE and graphite are both de-creased with the content increasing respectively. Consequently, compared with the PEI composite materials filled with graphite, the PEI composite materials filled with PTFE has more excellent tribological performance and mechanical behavior. The mechanical behavior of two kinds of the PEI composites was also measured, such as tensile strength, bending strength, impact strength and Rockwell hardness. The results show that, when the mass fraction of 5%—15%, which is the best tribological performance improvement in addition, comprehensive mechanical properties of blends of materials is still good.%将聚四氟乙烯( PTFE)和石墨两类减摩耐磨填料填充到聚醚酰亚胺( PEI)中,表征其摩擦性能,利用扫描电子显微镜分析了磨损表面的显微结构,并分析了磨损机制.研究结果表明, PTFE和石墨的填充明显改善了PEI的摩擦磨损性,摩擦系数降低到0.3以下(纯PEI的摩擦系数为0.41),磨损率降低了3个数量级.在PTFE体系中, PTFE

  19. SAN CARLOS APACHE PAPERS.

    Science.gov (United States)

    ROESSEL, ROBERT A., JR.

    THE FIRST SECTION OF THIS BOOK COVERS THE HISTORICAL AND CULTURAL BACKGROUND OF THE SAN CARLOS APACHE INDIANS, AS WELL AS AN HISTORICAL SKETCH OF THE DEVELOPMENT OF THEIR FORMAL EDUCATIONAL SYSTEM. THE SECOND SECTION IS DEVOTED TO THE PROBLEMS OF TEACHERS OF THE INDIAN CHILDREN IN GLOBE AND SAN CARLOS, ARIZONA. IT IS DIVIDED INTO THREE PARTS--(1)…

  20. San Carlo Operaen

    DEFF Research Database (Denmark)

    Holm, Bent

    2005-01-01

    En indplacering af operahuset San Carlo i en kulturhistorisk repræsentationskontekst med særligt henblik på begrebet napolalità.......En indplacering af operahuset San Carlo i en kulturhistorisk repræsentationskontekst med særligt henblik på begrebet napolalità....

  1. 丝尾鳠生物学特性及人工养殖技术%Biological Characteristics and Artificial Breeding Technology of Mystus wyckioides Chaux et Fang

    Institute of Scientific and Technical Information of China (English)

    张正兴; 张正雄; 薛晨江; 王文玉

    2014-01-01

    Morphological characteristics,life habits and breeding habits of Mystus wyckioides Chaux et Fang were expounded. Through the system discussion of aquarium construction,artificial reproduction technology,fish breeding technology,disease prevention and control technology of Mystus wyckioides Chaux et Fang,biological characteristics of Mystus wyckioides Chaux et Fang such as feeding habits,dissolved oxygen demand,temperature adaptability,salinity tolerance were verified,reproduction and fish cultivation measures of Mystus wyckioides Chaux et Fang were pointed out,pond farming required supporting technology and common disease control technology were also put forward.%阐述了丝尾鳠的形态特征、生活习性、繁殖习性。通过对丝尾鳠的养鱼池建造、人工繁殖技术、成鱼养殖技术、病害防治技术的系统论述,探明了丝尾鳠的食性、溶氧需求、温度适应性、盐度耐受性等生物学特性,指出了丝尾鳠繁殖与苗种培育措施,提出了池塘养殖所需配套技术及常见病害防治技术。

  2. Synthesis, and Characterization, and Evaluation of Cellular Effects of the FOL-PEG-g-PEI-GAL Nanoparticles as a Potential Non-Viral Vector for Gene Delivery

    Directory of Open Access Journals (Sweden)

    S. Ghiamkazemi

    2010-01-01

    Full Text Available In this manuscript, we synthesized the potential non viral vector for gene delivery with proper transfection efficiency and low cytotoxicity. Polyethylenimine (PEI is a well-known cationic polymer which has high positive surface charge for condensing plasmid DNA. However; it is highly cytotoxic in many cell lines because of the high surface charge, non-biodegradability and non-biocompatibility. To enhance PEI biodegradability, the graft copolymer “PEG-g-PEI” was synthesized. To target cancer liver cells, two targeting ligands folic acid and galactose (lactobionic acid which are over expressed on human hepatocyte carcinoma were attached to graft copolymer and “FOL-PEG-g-PEI-GAL” copolymer was synthesized. Composition of this grafted copolymer was characterized using 1H-NMR and FTIR spectra. The molecular weight and zeta potential of this copolymer was compared to PEI. The particle size and zeta potential of FOL-PEG-g-PEI-GAL/DNA complexes at various N/P ratio were measured using dynamic light scattering (DLS. Cytotoxicity of the copolymer was also studied in cultured HepG2 human hepatoblastoma cell line. The FOL-PEG-g-PEI-GAL/DNA complexes at various N/P ratios exhibited no cytotoxicity in HepG2 cell line compared to PEI 25K as a control. The novel copolymer showed enhanced biodegradability in physiological conditions in compared with PEI and targeted cultured HepG2 cells. More importantly, significant transfection efficiency was exhibited in cancer liver cells. Together, our results showed that “FOL-PEG-g-PEI-GAL” nanoparticals could be considered as a useful non-viral vector for targeted gene delivery.

  3. The alleviating pain effect of aqueous extract from tong-xie-yao-fang, on experimental visceral hypersensitivity and its mechanism.

    Science.gov (United States)

    Hu, Xu-Guang; Xu, Di; Zhao, Yue; Yang, Xiang-Bo; Meng, Jiang; Shen, Han; Guo, Jiao

    2009-06-01

    Tong-Xie-Yao-Fang (TXYF) is a prescription in traditional chinese medicine (TCM), used for relieving abdominal pain associated with irritable bowel syndrome. The aim of the present study was to investigate the effects and mechanism of TXYF on experimental visceral hypersensitivity (VH) models. TXYF affected the abdominal withdrawal reflex produced by colonic distention in maternal separation-induced visceral hypersensitivity rats, in a dosage-dependent manner. TXYF significantly decreased serotonin (5-HT) levels in serum and corticotrophin releasing factor (CRF) concentrations in the brain. Moreover, it was found that VH alleviation by TXYF was dependent on the substance P (SP) expression in the colon mucosa. These results suggest that TXYF attenuates behavioral hyperalgesia by regulating substance associated with the brain-gut axis, including decreasing the expression of 5-HT and SP in the periphery and that of CRF in the center.

  4. Haemocytic leukemia in Prince Edward Island (PEI) soft shell clam (Mya arenaria): Spatial distribution in agriculturally impacted estuaries

    Energy Technology Data Exchange (ETDEWEB)

    Muttray, Annette, E-mail: amuttray@rescan.com [Environment Canada, Water Science and Technology Directorate, 867 Lakeshore Road, Burlington, ON, Canada L7R 4A6 (Canada); Reinisch, Carol, E-mail: creinisch@mbl.edu [Visiting Scientist Environment Canada, Water Science and Technology Directorate, 867 Lakeshore Road, Burlington, ON, Canada L7R 4A6 (Canada); Miller, Jason, E-mail: j.miller@ec.gc.ca [Environment Canada, Water Science and Technology Directorate, 867 Lakeshore Road, Burlington, ON, Canada L7R 4A6 (Canada); Ernst, William, E-mail: bill.ernst@ec.gc.ca [Environment Canada, Water Science and Technology Directorate, 45 Alderney Drive, Dartmouth, NS, Canada B2Y 2N6 (Canada); Gillis, Patricia, E-mail: patty.gillis@ec.gc.ca [Environment Canada, Water Science and Technology Directorate, 867 Lakeshore Road, Burlington, ON, Canada L7R 4A6 (Canada); Losier, Melanie, E-mail: melanie.losier@ec.gc.ca [Environment Canada, Atlantic Laboratory for Environmental Testing, Environmental Science Centre, P.O. Box 23005 Moncton, NB, Canada E1A 6S8 (Canada); Sherry, James, E-mail: jim.sherry@ec.gc.ca [Environment Canada, Water Science and Technology Directorate, 867 Lakeshore Road, Burlington, ON, Canada L7R 4A6 (Canada)

    2012-05-01

    Intensive farming of potatoes in Prince Edward Island (PEI) relies on the repeated and widespread application of fertilizers and pesticides. In PEI the main potato farming areas are in close proximity and drain directly to estuaries. Runoff from high agricultural activity watersheds could impact benthic organism health in the depositional zone of downstream estuaries. The estuarine filter feeder Mya arenaria (soft-shell clam) could be particularly vulnerable to both particle-adsorbed and water soluble contaminants. M. arenaria is susceptible to haemocytic leukemia. In May 2009, we established that heavily proliferated leukemia (HPL) prevalence was generally higher in PEI estuaries located downstream of high intensity potato farming (Dunk and Wilmot estuaries) watersheds than in estuaries downstream of lower intensity areas. Using Mab-1E10 based immunocytochemistry we observed that leukemic haemocytes from the Dunk and Wilmot estuaries were 1E10 negative whereas those from the Ox/Sheep estuary (low potato farming intensity) were 1E10 positive. The expression of genes in the p53 tumour suppressor pathway enabled us to differentiate groups of leukemic and normal M. arenaria, validating our diagnoses. In October 2009, we confirmed that HPL prevalence was elevated in the Dunk and Wilmot estuaries compared to reference (Souris River). Moreover, leukemia prevalence declined with distance from the river mouths along transects through the Dunk and Wilmot estuaries. The pesticides ss-endosulfan and {alpha}-endosulfan were detected in surface sediments from the Dunk and Wilmot estuaries, but not in sediments from either the Souris River or several other lower intensity potato farming watersheds. Our study provides evidence of an association between intensity of potato farming and prevalence of clam leukemia at downstream estuaries in PEI. - Highlights: Black-Right-Pointing-Pointer We examined leukemia prevalence in PEI clams Mya arenaria. Black

  5. Analgesic activity of DaChuanXiongFang after intranasal administration and its potential active components in vivo.

    Science.gov (United States)

    Guo, Jianming; Pan, Weiwei; Qian, Dawei; Duan, Jin-ao; Shang, Erxin; Tang, Yuping

    2013-11-25

    DaChuanXiongFang was a well-known formula originated from Jin Dynasty, China. It has been used in both China and Japan to treat migraine. In the present study, the analgesic and sedative efficacy of DaChuanXiongFang ethanol extract (DCXFEE) after intranasal administration was tested and compared with that by intragastric route. Three mice experimental models: acetic acid-induced writhing response test, hot-plate latent pain response test and pentobarbital-induced sleep model were used to evaluate DCXFEE activity. To further explore the in vivo potential active components of DCXFEE that contribute to the difference of activity induced by different administration route, ultra performance liquid chromatography-mass spectrometer (UPLC-MS) was utilized to analyze components in rat brain after given DCXFEE (60 mg/kg). DCXFEE showed analgesic efficacy after intranasal administration (15, 30 and 60 mg/kg) in acetic acid-induced writhing response in mice. While after intragastric administration, DCXFEE only showed analgesic efficacy at high dose (60 mg/kg). Moreover, the analgesic potency was weaker after intragastric administration compared with that after intranasal administration at the same dose (60 mg/kg). Similar results were obtained in hot-plate latent pain response test in mice. DCXFEE (60 mg/kg) had no sedative effect after intranasal and intragastric administration. No components originated from DCXFEE were identified in rat brain 15 min after oral administration. One major parent component ligustilide was detected in rat brain after intranasal administration. These data demonstrate that DCXFEE had faster onset of action as well as better analgesic efficacy after intranasal administration than that after intragastric administration. DCXFEE has no sedative activity on potentiation of pentobarbital-induced sleep in mice given by both routes. Ligustilide might represents the potential major bioactive component of DCXFEE after intranasal administration and contribute

  6. Design Techniques of FANG Xuegu's Fashion Illustration%方雪鸪服装画的设计技法

    Institute of Scientific and Technical Information of China (English)

    张竞琼; 林舒琴; 谭莹

    2016-01-01

    探讨了方雪鸪服装画的风格,将其服装画的设计技法归结为素描、水彩和色粉画法3种;并从形体、妆容和配饰3方面分析了他对女性形象的构建,将其服装画的风格概括为优雅浪漫、图文并茂和巧妙布局.认为民国时期的绘画艺术、报刊媒介和服饰流行构成了对其服装画的根本影响.%Xuegu FANG,a famous painter and fashion designer in the history of modern China,got great achievement in fashion illustration field.This article mainly discusses about the style of Xuegu FANG's fashion illustration.Through the research,we summarize his drawing skills as three types: sketch,watercolor and crayon.We analyze his construction methods for female image from three aspects: body,makeup and ornament.Besides,his fashion illustration styles are sum up as elegant and romantic,excellent in both pictures and literary composition,as well as sophisticated layout.At the same time,we judge that the painting skills,the newspapers and magazines,and the clothing fashion of the republican period of China made important impact on his fashion illustrations.

  7. miRNA oligonucleotide and sponge for miRNA-21 inhibition mediated by PEI-PLL in breast cancer therapy.

    Science.gov (United States)

    Gao, Shiqian; Tian, Huayu; Guo, Ye; Li, Yuce; Guo, Zhaopei; Zhu, Xiaojuan; Chen, Xuesi

    2015-10-01

    MicroRNA-21 (miR-21) inhibition is a promising biological strategy for breast cancer therapy. However its application is limited by the lack of efficient miRNA inhibitor delivery systems. As a cationic polymer transfection material for nucleic acids, the poly (l-lysine)-modified polyethylenimine (PEI-PLL) copolymer combines the high transfection efficiency of polyethylenimine (PEI) and the good biodegradability of polyllysine (PLL). In this work, PEI-PLL was successfully synthesized and confirmed to transfect plasmid and oligonucleotide more effectively than PEI in MCF-7 cells (human breast cancer cells). In this regard, two kinds of miR-21 inhibitors, miR-21 sponge plasmid DNA (Sponge) and anti-miR-21 oligonucleotide (AMO), were transported into MCF-7 cells by PEI-PLL respectively. The miR-21 expression and the cellular physiology were determined post transfection. Compared with the negative control, PEI-PLL/Sponge or PEI-PLL/AMO groups exhibited lower miR-21 expression and cell viability. The anti-tumor mechanism of PEI-PLL/miR-21 inhibitors was further studied by cell cycle and western blot analyses. The results indicated that the miR-21 inhibition could induce the cell cycle arrest in G1 phase, upregulate the expression of Programmed Cell Death Protein 4 (PDCD4) and thus active the caspase-3 apoptosis pathway. Interestingly, the PEI-PLL/Sponge and PEI-PLL/AMO also sensitized the MCF-7 cells to anti-tumor drugs, doxorubicin (DOX) and cisplatin (CDDP). These results demonstrated that PEI-PLL/Sponge and PEI-PLL/AMO complexes would be two novel and promising gene delivery systems for breast cancer gene therapy based on miR-21 inhibition. This work was a combination of the high transfection efficiency of polyethylenimine (PEI), the good biodegradability of polyllysine (PLL) and the breast cancer-killing effect of miR-21 inhibitors. The poly (l-lysine)-modified polyethylenimine (PEI-PLL) copolymer was employed as the vector of miR-21 sponge plasmid DNA (Sponge) or

  8. 防风通圣散治疗肥胖症的研究现状%Fang Feng Tong Sheng San Status of Treatment of Obesity

    Institute of Scientific and Technical Information of China (English)

    牛蔚露; 万斌

    2009-01-01

    国内外学者就防风通圣散治疗肥胖症进行了较为深入的研究,取得了不少成果.查阅近30年的相关文献报道,分别从动物试验和临床研究两个方面就其现状予以综述.通过整理分析文献发现:(1)相关文献数量较少;(2)动物实验文献更少,且多数由国外学者完成;(3)早期临床试验不尽规范;(4)防风通圣散是中医临床治疗肥胖症的常用方剂,其疗效确切,安全性高,值得深入研究.

  9. Evaluation of the Passive Cooling Strategies for Pei Min Sport Complex

    Science.gov (United States)

    Yam, K. S.; Yem, W. L.; Lee, V. C. C.

    2017-07-01

    This paper presents a modelling study on the evaluation of the passive cooling strategies for Pei Min sport complex at Miri. The squash centre has experienced excessively high temperature during peak hours that results in complains from the users. We discussed several passive cooling mechanisms and proposed four strategies for the sport centre. Thermal energy simulations were performed on these strategies using OpenStudio to evaluate their impact on the hourly temperature profile within the building. It was found that the peak temperature during the noon was significantly reduced when conductive material was applied at the lower surface of the roof, and the top of the roof was coated with white paint. However, insulating the roof also leads to weaker heat dispersion from the building which lower the rate of temperature drop in the late afternoon. Partitioning the roof was found to have similar effect as insulating roof. Air infiltration is essential for promoting air movement and regulating the temperature within the building. It was found the complex already have sufficient opening for the full effect of air infiltration.

  10. PEI De-kai (裴德恺)A Prominent Pathophysiologist

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@PEI De-kai, male, was born in Shanghai, China, on June 8, 1928. His native place was Danyang, Jiangsu Province and he was graduated from Dalian Medical College in 1954. He holds some posts in several departments, such as the pathophysiologic professor, the academic comittee member and the head of basic research section in the Institute of Acute Abdomen in Dalian Medical University. His titles in learned society were the deputy-director of the Acute Abdomen Speciality Committee, Chinese Association of the Integration of Traditional and Western Medicine, and also in its Liaoning Branch; the deputy-director of Digestion Committee in Chinese Association of Pathophysiology, and the director of its branch in Dalian. He also holds some concurrent posts, such as the sanitary specialist of consultative committee and the researcher specially invited by Dalian municipality. As a professor, research fellow, and consultant, he was appointed by China Academy of Traditional Chinese Medicine, Dalian Institute of Medical Science, the Hospital of Integration of Traditional and Western Medicine in Dalian, and the Gallstone Research Center of State Administration of Traditional Chinese Medicine respectively.

  11. Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro

    Institute of Scientific and Technical Information of China (English)

    Gu Ping TANG; Zhi Yu WANG

    2006-01-01

    The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa.Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5 (mol/mol). The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.

  12. A Study on Zhu Pei's View on The Book of Songs%朱霈诗经学论略

    Institute of Scientific and Technical Information of China (English)

    朱宏胜

    2012-01-01

    朱霈治《诗》颇具怀疑精神,其以《氓》为异国之人淫于外州;《鸡鸣》为太师所奏乐曲;《无衣》为关武公;《采蘩》非夫人亲蚕之作;《汉广》诗与婚嫁有关。如此等等,敢于质疑前贤,自出新解。%Zhu Pei had the spirit of skepticism when he studied The Book of Songs. In his view, Meng is a poem which portraits a stranger in Wei; Ji Ming is a piece of music played by senior grand tutors; Wu Yi is a poem which praises Wu Gong; Hart Guang is a piece of poetry related to marriage, etc. Zhu Pei dared to question his predecessors, and speak out his own opinion.

  13. Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery.

    Science.gov (United States)

    Siu, King Sun; Zhang, Yujuan; Zheng, Xiufen; Koropatnick, James; Min, Wei-Ping

    2016-01-01

    The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo.

  14. Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells.

    Science.gov (United States)

    Lv, Juan; Hao, Xuefang; Li, Qian; Akpanyung, Mary; Nejjari, Abdelilah; Neve, Agnaldo Luis; Ren, Xiangkui; Feng, Yakai; Shi, Changcan; Zhang, Wencheng

    2017-01-17

    In this work, a biodegradable star-shaped copolymer poly(lactide-co-3(S)-methyl-morpholine-2,5-dione)6 (Star-(PLMD)6) was synthesized via ring-opening polymerization (ROP), and subsequently a gene carrier Star-PLMD-g-PEI-g-PEG-CREDVW was prepared by grafting polyethyleneimine (PEI), polyethylene glycol (PEG) and targeting peptide REDV onto Star-(PLMD)6. This gene carrier could form stable micelles to condense pEGFP-ZNF580 through electrostatic interaction. The resulting complexes were biocompatible and showed high efficiency in gene delivery. In addition, these complexes exhibited high selectivity for endothelial cells (ECs), high transfection efficiency and enhanced migration of ECs. The protein level of ZNF580 expression was significantly high (up to 85%), while the control group was only 51%. This combination of degradability, targeting ligand and star-structure strategy exhibits a significant advantage in transfection efficiency and migration of ECs.

  15. Two-Sided Surface Oxidized Cellulose Membranes Modified with PEI: Preparation, Characterization and Application for Dyes Removal

    OpenAIRE

    Wei Wang; Qian Bai; Tao Liang; Huiyu Bai; Xiaoya Liu

    2017-01-01

    Porous regenerated cellulose (RC) membranes were prepared with cotton linter pulp as a raw material. These membranes were first oxidized on both sides by a modified (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) oxidation system using a controlled oxidation reaction technique. Then, the oxidized RC membranes were functionalized with polyethylenimine (PEI) via the glutaraldehyde crosslinking method to obtain bifunctional (carboxyl and amino) porous RC membranes, as revealed by Fourier transfo...

  16. The Effect of PEI and PVP-Stabilized Gold Nanoparticles on Equine Platelets Activation: Potential Application in Equine Regenerative Medicine

    Directory of Open Access Journals (Sweden)

    Mateusz Hecold

    2017-01-01

    Full Text Available The aim of this work was to assess the effect of different stabilizing agents, for example, polyethylenimine (PEI and polyvinylpyrrolidone (PVP, on gold nanoparticles (AuNPs and their influence on equine platelet activation and release of particular growth factors. The gold nanoparticles were produced by chemical reduction of chloroauric acid. UV-Vis spectroscopy confirmed the presence of gold nanoparticles in investigated solutions. The AuNPs were incubated with whole blood at various concentrations. The morphology of platelets in PRP prepared from the blood incubated with AuNPs was characterized by scanning transmission electron microscopy, whereas the concentrations of growth factors and cytokines were evaluated by ELISA assays. The most promising results were obtained with equine platelets incubated with 5% AuNPs stabilized by PEI, which lead to secretion of bone morphogenetic protein 2 (BMP-2, vascular endothelial growth factor (VEGF, and fibroblast growth factor 1 (FGF-1 and simultaneously cause decrease in concentration of interleukin-1 alpha (IL-1α. The qRT-PCR confirmed ELISA test results. The incubation with 5% AuNPs stabilized by PEI leads to upregulation of BMP-2 and VEGF transcripts of mRNA level and to downregulating expression of interleukin-6 (IL-6. Obtained data shed a promising light on gold nanoparticle application for future regenerative medicine application.

  17. Electric Field Simulation and Effect of Different Solvent Ratios on the Performance of Single Electrospun PVDF/PEI Composite Film

    Directory of Open Access Journals (Sweden)

    Jin-gang Jiang

    2016-01-01

    Full Text Available On the basis of the finite element calculation theory of electric field, the electric field distribution in a representative electrospinning device is computed. The electric field structure of a needle-plate type electrospinning device was simulated by means of ANSYS software. And the vector distribution of the nozzle on the spinneret pipe was got. For the purpose of the analysis on the influence of different solvent ratios on the performance of a single electrospun PVDF/PEI composite film, polyvinylidene fluoride and polyetherimide with a mass ratio of 8/2 were dissolved in a mixed solvent. The mixed solvent is composed of N,N-dimethylformamide and tetrahydrofuran, added in different proportions. Through the electrostatic spinning technology, PVDF/PEI composite fiber membranes were prepared. Using scanning electron microscopy (SEM, X-ray diffraction (XRD, differential scanning calorimetry (DSC, and mechanical properties testing, the effects of tetrahydrofuran on the composite microstructure, crystallinity, and mechanical properties of the PVDF/PEI composite fiber membranes are discussed.

  18. Low Molecular Weight PEI-Based Vectors via Acid-Labile Ortho Ester Linkage for Improved Gene Delivery.

    Science.gov (United States)

    Zhang, Lei; Yu, Min; Wang, Jun; Tang, Rupei; Yan, Guoqing; Yao, Weijing; Wang, Xin

    2016-08-01

    A series of novel pH-sensitive gene delivery vectors (POEI 1, 2, and 3) are synthesized through Michael addition from low molecular weight PEI (LMW PEI) via acid-labile ortho ester linkage with terminal acrylates (OEAc) by various feed molar ratios. The obtained POEI 1 and POEI 2 can efficiently condense plasmid DNA into nanoparticles with size range of 200-300 nm and zeta-potentials of about +15 mV while protecting DNA from enzymatic digestion compared with POEI 3. Significantly, ortho ester groups of POEI main-chains can make an instantaneous degradation-response to acidic endosomal pH (≈5.0), resulting in accelerated disruption of polyplexes and intracellular DNA release. MTT assay reveals that all POEIs exhibit much lower cytotoxicity in different cells than branched PEI (25 KDa). As expected, POEI 1 and POEI 2 perform improved gene transfection in vitro, suggesting that such polycations might be promising gene vectors based on overcoming toxicity-efficiency contradiction.

  19. Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate

    Directory of Open Access Journals (Sweden)

    Xiangyou Wang

    2013-04-01

    Full Text Available In this paper, an amperometric immunosensor for the detection of carbofuran was developed. Firstly, multiwall carbon nanotubes (MWCNTs and graphene sheets-ethyleneimine polymer-Au (GS-PEI-Au nanocomposites were modified onto the surface of a glass carbon electrode (GCE via self-assembly. The nanocomposites can increase the surface area of the GCE to capture a large amount of antibody, as well as produce a synergistic effect in the electrochemical performance. Then the modified electrode was coated with gold nanoparticles-antibody conjugate (AuNPs-Ab and blocked with BSA. The monoclonal antibody against carbofuran was covalently immobilized on the AuNPs with glutathione as a spacer arm. The morphologies of the GS-PEI-Au nanocomposites and the fabrication process of the immunosensor were characterized by X-ray diffraction (XRD, ultraviolet and visible absorption spectroscopy (UV-vis and scanning electron microscopy (SEM, respectively. Under optimal conditions, the immunosensor showed a wide linear range, from 0.5 to 500 ng/mL, with a detection limit of 0.03 ng/mL (S/N = 3. The as-constructed immunosensor exhibited notable performance features such as high specificity, good reproducibility, acceptable stability and regeneration performance. The results are mainly due to the excellent properties of MWCNTs, GS-PEI-Au nanocomposites and the covalent immobilization of Ab with free hapten binding sites for further immunoreaction. It provides a new avenue for amperometric immunosensor fabrication.

  20. Amperometric immunosensor for carbofuran detection based on MWCNTs/GS-PEI-Au and AuNPs-antibody conjugate.

    Science.gov (United States)

    Zhu, Ying; Cao, Yaoyao; Sun, Xia; Wang, Xiangyou

    2013-04-19

    In this paper, an amperometric immunosensor for the detection of carbofuran was developed. Firstly, multiwall carbon nanotubes (MWCNTs) and graphene sheets-ethyleneimine polymer-Au (GS-PEI-Au) nanocomposites were modified onto the surface of a glass carbon electrode (GCE) via self-assembly. The nanocomposites can increase the surface area of the GCE to capture a large amount of antibody, as well as produce a synergistic effect in the electrochemical performance. Then the modified electrode was coated with gold nanoparticles-antibody conjugate (AuNPs-Ab) and blocked with BSA. The monoclonal antibody against carbofuran was covalently immobilized on the AuNPs with glutathione as a spacer arm. The morphologies of the GS-PEI-Au nanocomposites and the fabrication process of the immunosensor were characterized by X-ray diffraction (XRD), ultraviolet and visible absorption spectroscopy (UV-vis) and scanning electron microscopy (SEM), respectively. Under optimal conditions, the immunosensor showed a wide linear range, from 0.5 to 500 ng/mL, with a detection limit of 0.03 ng/mL (S/N = 3). The as-constructed immunosensor exhibited notable performance features such as high specificity, good reproducibility, acceptable stability and regeneration performance. The results are mainly due to the excellent properties of MWCNTs, GS-PEI-Au nanocomposites and the covalent immobilization of Ab with free hapten binding sites for further immunoreaction. It provides a new avenue for amperometric immunosensor fabrication.

  1. Formation and characterization of β-cyclodextrin (β-CD) - polyethyleneglycol (PEG) - polyethyleneimine (PEI) coated Fe3O4 nanoparticles for loading and releasing 5-Fluorouracil drug.

    Science.gov (United States)

    Prabha, G; Raj, V

    2016-05-01

    In this work, β-cyclodextrin (β-CD) - polyethyleneglycol (PEG) - polyethyleneimine (PEI) coated iron oxide nanoparticles (Fe3O4-β-CD-PEG-PEI) were developed as drug carriers for drug delivery applications. The 5- Fluorouracil (5-FU) was chosen as model drug molecule. The developed nanoparticles (Fe3O4-β-CD-PEG-PEI) were characterized by various techniques such as Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM). The average particles size range of 5-FU loaded Fe3O4-β-CD, Fe3O4-β-CD-PEG and Fe3O4-β-CD-PEG-PEI nanoparticles were from 151 to 300nm and zeta potential value of nanoparticles were from -43mV to -20mV as measured using Malvern Zetasizer. Finally, encapsulation efficiency (EE), loading capacity (LC) and in-vitro drug release performance of 5-FU drug loaded Fe3O4-β-CD, Fe3O4-β-CD-PEG and Fe3O4-β-CD-PEG-PEI nanoparticles was evaluated by UV-vis spectroscopy. In-vitro cytotoxicity tests investigated by MTT assay indicate that 5-FU loaded Fe3O4-β-CD-PEG-PEI nanoparticles were toxic to cancer cells and non-toxic to normal cells. The in-vitro release behavior of 5-FU from drug (5-FU) loaded Fe3O4-β-CD-PEG-PEI composite at different pH values and temperature was studied. It was found that 5-FU was released faster in pH 6.8 than in the acidic mediums (pH 1.2), and the released quantity was higher. Therefore, the newly prepared Fe3O4-β-CD-PEG-PEI carrier exhibits a promising potential capability for anticancer drug delivery in tumor therapy.

  2. PEI-engineered respirable particles delivering a decoy oligonucleotide to NF-κB: inhibiting MUC2 expression in LPS-stimulated airway epithelial cells.

    Directory of Open Access Journals (Sweden)

    Francesca Ungaro

    Full Text Available A specific and promising approach to limit inflammation and mucin iperproduction in chronic lung diseases relies on specific inhibition of nuclear Factor-κB (NF-κB by a decoy oligonucleotide (dec-ODN. To fulfill the requirements dictated by translation of dec-ODN therapy in humans, inhalable dry powders were designed on a rational basis to provide drug protection, sustained release and to optimize pharmacological response. To this end, large porous particles (LPP for dec-ODN delivery made of a sustained release biomaterial (poly(lactic-co-glycolic acid, PLGA and an "adjuvant" hydrophilic polymer (polyethylenimine, PEI were developed and their effects on LPS-stimulated human airway epithelial cells evaluated. The composite PLGA/PEI particles containing dec-ODN (i.e., LPP(PEI were successfully engineered for widespread deposition in the lung and prolonged release of intact dec-ODN in vitro. LPP(PEI caused a prolonged inhibition of IL-8 and MUC2 expression in CF human bronchial epithelial cells and human epithelial pulmonary NCI-H292 cells, respectively, as compared to naked dec-ODN. Nonetheless, as compared to previously developed LPP, the presence of PEI was essential to construct a dec-ODN delivery system able to act in mucoepidermoid lung epithelial cells. In perspective, engineering LPP with PEI may become a key factor for tuning carrier properties, controlling lung inflammation and mucin production which, in turn, can foster in vivo translation of dec-ODN therapy.

  3. Effect of Yishen Qiqiao Fang on dopamine content in serum and cerebrospinal fluid of patients in persistent vegetative statec

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    BACKGROUND: It has been demonstrated that the attack of persistent vegetative state is associated with the damaged neuron, which produces dopamine, and nervous pathway, the reduce of dopamine or malfunction of dopamine.OBJECTIVE: To observe the effect of self-made traditional Chinese medicine (TCM) Yishen Qiqiao Fang,which has the functions of supplementing qi and nourishing blood, resolving phlegm by promoting blood circulation, restoring consciousness and inducing resuscitation, on the contents of dopamine in serum and cerebrospinal fluid of patients in persistent vegetative state.DESIGN: An open randomized controlled clinical trial.SETTINGS: Nanjing University of Traditional Chinese Medicine, Nanjing Zijin Hospital.PARTICIPANTS: Thirty-eight inpatients of persistent vegetative state were selected from the Department of Neurology, Nanjing Zijin Hospital from August 2005 to November 2006. The patients were diagnosed according to the diagnostic standards set by the summary of a meeting for specialists in Nanjing. Informed contents were obtained from their relatives. According to the order of admission, the enrolled patients were divided into control group (n =20) and TCM treated group (n =20).METHODS: In the control group, the patients were treated with routine treatments for the symptoms. In the TCM treated group, the patients were treated with Yishen Qiqiao Fang besides the same treatments in the control group. TCM dispensing granules: each bag of mongolian milkvetch root, Chinese angelica and peach seed equaled to 10 g crude drug respectively; each bag of grassleaf sweetflag rhizome and dahurian angelica root equaled to 6 g crude drug respectively; Musk 0.05 g. The daily dosage for adults: 4 bags of mongolian milkvetch root, 2 bags of Chinese angelica, 0.05 g musk, 1 bag of peach seed, 2 bags of grassleaf sweetflag rhizome and 2 bags of dahurian angelica root, which should be given though nasal feeding or gastrostogavage before breakfast and supper every day

  4. Developmental and reproductive toxicity of PVP/PEI-coated silver nanoparticles to zebrafish.

    Science.gov (United States)

    Orbea, Amaia; González-Soto, Nagore; Lacave, José María; Barrio, Irantzu; Cajaraville, Miren P

    2017-09-01

    Cellular and molecular mechanisms of toxicity of silver nanoparticles (NPs) and their toxicity to fish embryos after waterborne exposure have been widely investigated, but much less information is available regarding the effect of Ag NPs on physiological functions such as growth or reproduction. In this work, the effects of waterborne exposure of adult zebrafish (Danio rerio) to PVP/PEI coated Ag NPs (~5nm) on reproduction (fecundity) were investigated. Moreover, the development of the embryos after parental exposure was compared with the development of embryos after direct waterborne exposure to the NPs. For this, two experiments were run: 1) embryos from unexposed parents were treated for 5days with Ag NPs (10μgAgL(-1)-10mgAgL(-1)) and development was monitored, and 2) selected breeding zebrafish were exposed for 3weeks to 100ngAgL(-1) (environmentally relevant concentration) or to 10μgAgL(-1) of Ag NPs, fecundity was scored and development of resulting embryos was monitored up to 5days. Waterborne exposure of embryos to Ag NPs resulted in being highly toxic (LC50 at 120h=50μgAgL(-1)), causing 100% mortality during the first 24h of exposure at 0.1mgAgL(-1). Exposure of adults, even at the environmentally relevant silver concentration, caused a significant reduction of fecundity by the second week of treatment and resulting embryos showed a higher prevalence of malformations than control embryos. Exposed adult females presented higher prevalence of vacuolization in the liver. These results show that Ag NPs at an environmentally relevant concentration are able to affect population level parameters in zebrafish. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Screening and characterization of ethanol-tolerant and thermotolerant acetic acid bacteria from Chinese vinegar Pei.

    Science.gov (United States)

    Chen, Yang; Bai, Ye; Li, Dongsheng; Wang, Chao; Xu, Ning; Hu, Yong

    2016-01-01

    Acetic acid bacteria (AAB) are important microorganisms in the vinegar industry. However, AAB have to tolerate the presence of ethanol and high temperatures, especially in submerged fermentation (SF), which inhibits AAB growth and acid yield. In this study, seven AAB that are tolerant to temperatures above 40 °C and ethanol concentrations above 10% (v/v) were isolated from Chinese vinegar Pei. All the isolated AAB belong to Acetobacter pasteurianus according to 16S rDNA analysis. Among all AAB, AAB4 produced the highest acid yield under high temperature and ethanol test conditions. At 4% ethanol and 30-40 °C temperatures, AAB4 maintained an alcohol-acid transform ratio of more than 90.5 %. High alcohol-acid transform ratio was still maintained even at higher temperatures, namely, 87.2, 77.1, 14.5 and 2.9% at 41, 42, 43 and 44 °C, respectively. At 30 °C and different initial ethanol concentrations (4-10%), the acid yield by AAB4 increased gradually, although the alcohol-acid transform ratio decreased to some extent. However, 46.5, 8.7 and 0.9% ratios were retained at ethanol concentrations of 11, 12 and 13%, respectively. When compared with AS1.41 (an AAB widely used in China) using a 10 L fermentor, AAB4 produced 42.0 g/L acetic acid at 37 °C with 10% ethanol, whereas AS1.41 almost stopped producing acetic acid. In conclusion, these traits suggest that AAB4 is a valuable strain for vinegar production in SF.

  6. Prescription Rules of Huagan Tongluo Fang%化肝通络方组方规律探讨

    Institute of Scientific and Technical Information of China (English)

    李忠意; 田耀洲

    2014-01-01

    Huagan Tongluo Fang, originated from a famous prescription“Lang Qing A Ta” in Tibetan medicine by Pro-fessor TIAN Yao-zhou, has a significant effect on primary biliary cirrhosis in clinic.The prescription fuses traditional Chinese medicine and Tibetan medicine, having a distinct compatibility, with saffron and artificial cow bezoar as the monarch for nourishing liver and dredging collaterals, flying squirrel faeces, India swertia and emblic leafflower fruit as minister for clearing away liver heat and soothing blood to nourish liver, milk-vetch root, nutmeg, dolomiaea root, aq-uilaria wood and liquorice root as assistant and guide for invigorating spleen to regulate qi.This paper systematically ex-plains prescription rules of Huagan Tongluo Fang, modern pharmacology researches and methods for treating liver and analyzes the prescription rules mainly based on clearing heat, eliminating stasis, dredging collaterals and tonifying spleen from the perspective of theory of traditional Chinese medicine and modern pharmacological research to make a conclusion that the core of the prescription is to nourish liver and dredge collaterals, soothe blood to nourish liver, treat liver and spleen together.%化肝通络方是由田耀洲教授化裁藏医治肝名方“郎庆阿塔”而成,在临床上治疗原发性胆汁淤积性肝硬化( PBC)效果显著。处方融合传统中药、藏药,配伍鲜明,以西红花、人工牛黄为君药,化肝通络;以五灵脂、印度獐牙菜、余甘子为臣药,清化肝热、行血养肝;佐使黄芪、肉豆蔻、木香、沉香、甘草等健脾理气。本文从中医理论角度及现代药理学研究出发,对化肝通络方组方理论依据、现代药理学研究、治肝之法等方面进行系统阐述,分析其组方规律,以清热、化瘀、通络、健脾为主线,总结其组方核心为化肝通络,行血养肝,肝脾同治。

  7. Principle Analysis of Zhuanggu Fang in Treating Senile Osteoporosis%壮骨方治疗老年骨质疏松症的原理分析

    Institute of Scientific and Technical Information of China (English)

    吴群海

    2015-01-01

    Senile osteoporosis is a common disease.With aging population, the incidence rate increased year by year.Zhuanggu Fang has unique advantages in the prevention and treatment of primary osteoporosis.In this article, the author introduced the further development in therapy of primary osteoporosis by Zhuanggu Fang.%老年骨质疏松症是临床常见病。随着人口逐渐老龄化,发病率逐年升高。壮骨方在预防治疗原发性骨质疏松症有独到的优势。本文进一步壮骨方在治疗原发性骨质疏松症研究领域的进展。

  8. [PHEMA/PEI]–Cu(II) based immobilized metal affinity chromatography cryogels: Application on the separation of IgG from human plasma

    Energy Technology Data Exchange (ETDEWEB)

    Bakhshpour, Monireh; Derazshamshir, Ali; Bereli, Nilay [Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara (Turkey); Elkak, Assem [Laboratory of “Valorisation des Ressources Naturelles et Produits de Santé (VRNPS)”, Doctoral School of Sciences and Technology, Lebanese University, Rafic Hariri University Campus, Hadath (Lebanon); Denizli, Adil, E-mail: denizli@hacettepe.edu [Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara (Turkey)

    2016-04-01

    The immobilized metal-affinity chromatography (IMAC) has gained significant interest as a widespread separation and purification tool for therapeutic proteins, nucleic acids and other biological molecules. The enormous potential of IMAC for proteins with natural surface exposed-histidine residues and for recombinant proteins with histidine clusters. Cryogels as monolithic materials have recently been proposed as promising chromatographic adsorbents for the separation of biomolecules in downstream processing. In the present study, IMAC cryogels have been synthesized and utilized for the adsorption and separation of immunoglobulin G (IgG) from IgG solution and whole human plasma. For this purpose, Cu(II)-ions were coupled to poly(hydroxyethyl methacrylate) PHEMA using poly(ethylene imine) (PEI) as the chelating ligand. In this study the cryogels formation optimized by the varied proportion of PEI from 1% to 15% along with different amounts of Cu (II) as chelating metal. The prepared cryogels were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The [PHEMA/PEI]–Cu(II) cryogels were assayed for their capability to bind the human IgG from aqueous solutions. The IMAC cryogels were found to have high affinity toward human IgG. The adsorption of human IgG was investigated onto the PHEMA/PEI cryogels with (10% PEI) and the concentration of Cu (II) varied as 10, 50, 100 and 150 mg/L. The separation of human IgG was achieved in one purification step at pH 7.4. The maximum adsorption capacity was observed at the [PHEMA/PEI]–Cu(II) (10% PEI) with 72.28 mg/g of human IgG. The purification efficiency and human IgG purity were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). - Highlights: • Cu(II)-ions were coupled to PHEMA using PEI as the chelating ligand. • Cu(II) chelated [PHEMA/PEI] cryogels for IgG separation were produced. • Maximum IgG adsorption capacity

  9. RNA-seq and high-definition mass spectrometry reveal the complex and divergent venoms of two rear-fanged colubrid snakes.

    Science.gov (United States)

    McGivern, James J; Wray, Kenneth P; Margres, Mark J; Couch, Michelle E; Mackessy, Stephen P; Rokyta, Darin R

    2014-12-03

    Largely because of their direct, negative impacts on human health, the venoms of front-fanged snakes of the families Viperidae and Elapidae have been extensively characterized proteomically, transcriptomically, and pharmacologically. However, relatively little is known about the molecular complexity and evolution of the venoms of rear-fanged colubrid snakes, which are, with a few notable exceptions, regarded as harmless to humans. Many of these snakes have venoms with major effects on their preferred prey, and their venoms are probably as critical to their survival as those of front-fanged elapids and viperids. We sequenced the venom-gland transcriptomes from a specimen of Hypsiglena (Desert Night Snake; family Colubridae, subfamily Dipsadinae) and of Boiga irregularis (Brown Treesnake; family Colubridae, subfamily Colubrinae) and verified the transcriptomic results proteomically by means of high-definition mass spectrometry. We identified nearly 3,000 nontoxin genes for each species. For B. irregularis, we found 108 putative toxin transcripts in 46 clusters with definition transcriptomic and proteomic approaches. We found a high level of divergence in the venom types of two colubrids. These two venoms reflected the hemorrhagic/neurotoxic venom dichotomy that broadly characterizes the difference in venom strategies between elapids and viperids.

  10. [Tai ping sheng hui fang (Taiping Holy Prescriptions for Universal Relief) and the compilation during the early Song Dynasty].

    Science.gov (United States)

    Han, Yi

    2010-07-01

    Tai ping sheng hui fang, the first medical formulary of the Song Dynasty, compiled from the 3(rd) year of Taiping-xingguo reign to the 3(rd) year of Chunhua reign (978 ∼ 992), under the edict of the Taizong Emperor, was popularly applied and extensively circulated and called "the first formulary of the Dynasty". It is extremely significant in the medical history of the Song Dynasty due to its theory of prescription art, practical prescriptions and clinical practice. During the process of its circulation, different versions appeared, including the Guozijian Orthodox Version, Guozijian Small-character Version, Chongwen Hall Abridged Version, Newly Carved Version of Zhuanyunsi, and the Local Abridged Version, thus adapting to the demands of various walks of life in the society. Its unique role in the development of the Song society was founded by its introduction, application and popularization by the emperors, local officials, medical scholars, diplomatic envoys, and intellectuals. The "kind administration" of the authority and the government was further greatly facilitated by the involvement of the government and the introduction of printing. Its practical prescriptions became forceful tools to prevent and treat diseases, to conquer witchcraft, to protect local social security, and to pronounce the merits of officials and physicians at all levels.

  11. Tong Xie Yao Fang relieves irritable bowel syndrome in rats via mechanisms involving regulation of 5-hydroxytryptamine and substance P.

    Science.gov (United States)

    Yin, Yue; Zhong, Lei; Wang, Jian-Wei; Zhao, Xue-Ying; Zhao, Wen-Jing; Kuang, Hai-Xue

    2015-04-21

    To investigate whether the Chinese medicine Tong Xie Yao Fang (TXYF) improves dysfunction in an irritable bowel syndrome (IBS) rat model. Thirty baby rats for IBS modeling were separated from mother rats (1 h per day) from days 8 to 21, and the rectum was expanded by angioplasty from days 8 to 12. Ten normal rats were used as normal controls. We examined the effects of TXYF on defection frequency, colonic transit function and smooth muscle contraction, and the expression of 5-hydroxytryptamine (5-HT) and substance P (SP) in colonic and hypothalamus tissues by Western blot and RT-PCT techniques in both normal rats and IBS model rats with characterized visceral hypersensitivity. Defecation frequency was 1.8 ± 1.03 in normal rats and 4.5 ± 1.58 in IBS model rats (P colon transit function was significantly increased (256.88 ± 20.32 vs 93.36 ± 17.28, P colonic smooth muscle tension and contract frequency in IBS model rats were significantly decreased by administration of TXYF. Exogenous agonist stimulants increased spontaneous activity and elicited contractions of colon smooth muscle in IBS model rats, and all of these actions were significantly reduced by TXYF involving 5-HT and SP down-regulation. TXYF can modulate the activity of the enteric nervous system and alter 5-HT and SP activities, which may contribute to the symptoms of IBS.

  12. [PHEMA/PEI]-Cu(II) based immobilized metal affinity chromatography cryogels: Application on the separation of IgG from human plasma.

    Science.gov (United States)

    Bakhshpour, Monireh; Derazshamshir, Ali; Bereli, Nilay; Elkak, Assem; Denizli, Adil

    2016-04-01

    The immobilized metal-affinity chromatography (IMAC) has gained significant interest as a widespread separation and purification tool for therapeutic proteins, nucleic acids and other biological molecules. The enormous potential of IMAC for proteins with natural surface exposed-histidine residues and for recombinant proteins with histidine clusters. Cryogels as monolithic materials have recently been proposed as promising chromatographic adsorbents for the separation of biomolecules in downstream processing. In the present study, IMAC cryogels have been synthesized and utilized for the adsorption and separation of immunoglobulin G (IgG) from IgG solution and whole human plasma. For this purpose, Cu(II)-ions were coupled to poly(hydroxyethyl methacrylate) PHEMA using poly(ethylene imine) (PEI) as the chelating ligand. In this study the cryogels formation optimized by the varied proportion of PEI from 1% to 15% along with different amounts of Cu (II) as chelating metal. The prepared cryogels were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The [PHEMA/PEI]-Cu(II) cryogels were assayed for their capability to bind the human IgG from aqueous solutions. The IMAC cryogels were found to have high affinity toward human IgG. The adsorption of human IgG was investigated onto the PHEMA/PEI cryogels with (10% PEI) and the concentration of Cu (II) varied as 10, 50, 100 and 150 mg/L. The separation of human IgG was achieved in one purification step at pH7.4. The maximum adsorption capacity was observed at the [PHEMA/PEI]-Cu(II) (10% PEI) with 72.28 mg/g of human IgG. The purification efficiency and human IgG purity were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

  13. Efficient and Tumor Targeted siRNA Delivery by Polyethylenimine-graft-polycaprolactone-block-poly(ethylene glycol)-folate (PEI-PCL-PEG-Fol).

    Science.gov (United States)

    Liu, Li; Zheng, Mengyao; Librizzi, Damiano; Renette, Thomas; Merkel, Olivia M; Kissel, Thomas

    2016-01-04

    Efficient delivery of functional nucleic acids into specific cells or tissues is still a challenge for gene therapy and largely depends on targeted delivery strategies. The folate receptor (FR) is known to be overexpressed extracellularly on a variety of human cancers and is therefore an outstanding gate for tumor-targeted Trojan horse-like delivery of therapeutics. In this study, an amphiphilic and biodegradable ternary copolymer conjugated with folate as ligand, polyethylenimine-graft-polycaprolactone-block-poly(ethylene glycol)-folate (PEI-PCL-PEG-Fol) was synthesized and evaluated for targeted siRNA delivery via folate-FR recognition. The amphiphilic character of similar polymers was shown previously to support endosomal release of endocytosed nanocarriers and to promote formation of long circulating micelles. The obtained PEI-PCL-PEG-Fol exhibited less cytotoxicity in comparison with the corresponding ternary copolymer without folate (PEI-PCL-PEG) and with unmodified PEI25kDa. Stable micelle-like polyplexes with hydrodynamic diameters about 100 nm were found to have a zeta potential of +8.6 mV, which was lower than that of micelleplexes without folate-conjugation (+13-16 mV). Nonetheless, increased cellular uptake and in vitro gene knockdown of PEI-PCL-PEG-Fol/siRNA micelleplexes were observed in SKOV-3 cells, an FR overexpressing cell line, in comparison with the nonfolate-conjugated ones. Moreover, PEI-PCL-PEG-Fol/siRNA micelleplexes exhibited excellent stability in vivo during the analysis of 120 min and a longer circulation half life than hyPEI25kDa/siRNA polyplexes. Most interestingly, the targeted delivery system yielded 17% deposition of the i.v. injected siRNA per gram in the tumor after 24 h due to the effective folate targeting and the prolonged circulation.

  14. San Pascual (1989) n. 272

    OpenAIRE

    Pérez, María Dolores, O.S.C. (Directora)

    1989-01-01

    Editorial. Entrevista madre abadesa. Ofrenda. San Pascual tercer centenario de la canonizacion y cuarto de su muerte. San Pascual, un Santo universal. Pascual Baylón, poeta. grupo Scout Sant Pasqual. Aportaciones, donativos, limosnas, benefactores. Boletin informativo del templo de San Pascual de villareal.

  15. Shen-Qi-Jie-Yu-Fang has antidepressant effects in a rodent model of postpartum depression by regulating the immune organs and subsets of T lymphocytes

    Directory of Open Access Journals (Sweden)

    Qu M

    2015-06-01

    Full Text Available Miao Qu,1 Qisheng Tang,1 Xiaoli Li,1 Ruizhen Zhao,1 Jingya Li,1 Hong Xu,2 Yushan Gao,2 Yingqiu Mao31Third Affiliated Hospital, 2School of Basic Medical Sciences, 3Center of Scientific Research, Beijing University of Chinese Medicine, Beijing, People’s Republic of China Background: Shen-Qi-Jie-Yu-Fang (SJ Fang is a herbal preparation used in traditional Chinese medicine, and is a potentially important new therapeutic agent in postpartum depression (PPD. Previously, we have elucidated the effects of SJ Fang on hormone receptors and monoamine neurotransmitters involved in the hypothalamic-pituitary-adrenal and hypothalamic-pituitary-gonadal axes in PPD rats. However, the immune-modulating effects of SJ Fang in PPD are still unknown. In this study, we explored the effects of SJ Fang on the immune organs and subsets of T lymphocytes in PPD rats.Methods: PPD was created in Sprague-Dawley rats by inducing hormone-simulated pregnancy followed by hormone withdrawal. After hormone withdrawal, the PPD rats were then treated with fluoxetine at 1, 2, and 4 weeks, and the SJ Fang rats were also treated at 1, 2, and 4 weeks. Depressive behavior in the rats was evaluated by the forced swim test, sucrose consumption test, and open field test. The thymus index and spleen index were calculated. Hematoxylin-eosin staining was used to identify pathological features in the thymus and spleen. CD3, CD4, and CD8 lymphocyte subsets were analyzed by flow cytometry.Results: Both fluoxetine and SJ Fang increased immobility time, sucrose consumption, an horizontal and vertical movements. After 4 weeks of treatment with fluoxetine or SJ Fang, the thymus index and spleen index were significantly higher than at baseline, and the morphology of the thymus and spleen were returning to normal. Two weeks after hormone withdrawal, subsets of T lymphocytes indicated a shift from immune activation to immune suppression, which was reversed by 4 weeks of treatment with fluoxetine or SJ

  16. Aspectos clínicos do entrópio de desenvolvimento em cães da raça Shar Pei Clinical aspects of developmental entropion in Shar Pei dogs

    Directory of Open Access Journals (Sweden)

    F.A.B. Viana

    2006-04-01

    Full Text Available Avaliaram-se os aspectos clínicos do entrópio de desenvolvimento em filhotes de cães da raça Shar Pei. Utilizaram-se 50 animais com idades entre 18 e 128 dias, que apresentavam graus variáveis de inversão palpebral e lesões oculares, as quais foram classificadas de acordo com um modelo específico proposto. As lesões encontradas foram fotofobia, epífora, inversão da margem palpebral, blefarospasmo, conjuntivite, quemose, edema, erosão e vascularização da córnea e phthisis bulbi.The clinical aspects of entropion in puppies of the Shar Pei breed were studied using 50 animals, 18 to 128 day-old, that presented variable degrees of palpebral inversion and ocular lesions. The ocular lesions were classified according to a specific model. Photophobia, epiphora, palpebral margins inversion, blepharospasm, conjuntivitis, quemosis, corneal edema, erosion and vascularization and phthisis bulbi were observed.

  17. Metabolomic analysis of anti-hypoxia and anti-anxiety effects of Fu Fang Jin Jing Oral Liquid.

    Directory of Open Access Journals (Sweden)

    Xia Liu

    Full Text Available BACKGROUND: Herba Rhodiolae is a traditional Chinese medicine used by the Tibetan people for treating hypoxia related diseases such as anxiety. Based on the previous work, we developed and patented an anti-anxiety herbal formula Fu Fang Jin Jing Oral Liquid (FJJOL with Herba Rhodiolae as a chief ingredient. In this study, the anti-hypoxia and anti-anxiety effects of FJJOL in a high altitude forced-swimming mouse model with anxiety symptoms will be elucidated by NMR-based metabolomics. METHODS: In our experiments, the mice were divided randomly into four groups as flatland group, high altitude saline-treated group, high altitude FJJOL-treated group, and high altitude diazepam-treated group. To cause anxiety effects and hypoxic defects, a combination use of oxygen level decreasing (hypobaric cabin and oxygen consumption increasing (exhaustive swimming were applied to mice. After a three-day experimental handling, aqueous metabolites of mouse brain tissues were extracted and then subjected to NMR analysis. The therapeutic effects of FJJOL on the hypobaric hypoxia mice with anxiety symptoms were verified. RESULTS: Upon hypoxic exposure, both energy metabolism defects and disorders of functional metabolites in brain tissues of mice were observed. PCA, PLS-DA and OPLS-DA scatter plots revealed a clear group clustering for metabolic profiles in the hypoxia versus normoxia samples. After a three-day treatment with FJJOL, significant rescue effects on energy metabolism were detected, and levels of ATP, fumarate, malate and lactate in brain tissues of hypoxic mice recovered. Meanwhile, FJJOL also up-regulated the neurotransmitter GABA, and the improvement of anxiety symptoms was highly related to this effect. CONCLUSIONS: FJJOL ameliorated hypobaric hypoxia effects by regulating energy metabolism, choline metabolism, and improving the symptoms of anxiety. The anti-anxiety therapeutic effects of FJJOL were comparable to the conventional anti-anxiety drug

  18. The Therapeutic Effects of the Chinese Herbal Medicine, Lang Chuang Fang Granule, on Lupus-Prone MRL/lpr Mice

    Directory of Open Access Journals (Sweden)

    Kai-Peng Huang

    2016-01-01

    Full Text Available Systemic lupus erythematosus (SLE is a chronic autoimmune disease that leads to severe multiorgan damage. Lang Chuang Fang (LCF is a Chinese herbal medicine that is clinically prescribed for treating SLE. In this study, we examined the therapeutic effects of LCF granule on lupus-prone MRL/lpr mice. Female mice were randomly separated into six groups, and LCF treatment groups received LCF granule at the dosage of 0.97 g/kg/d, 1.95 g/kg/d, and 3.90 g/kg/d, respectively. Here, we found that, compared to the MRL/lpr mice, both the spleen coefficient and thymus coefficient were reduced in the LCF granule-treated mice. There was a marked downregulation in CRP and anti-dsDNA autoantibody and an evident upregulation of CH50 in LCF granule-treated mice. LCF granule treatment also obviously reduced the proteinuria, BUN, and SCr levels in MRL/lpr mice at the dosage of 0.97 g/kg/d, 1.95 g/kg/d, and 3.90 g/kg/d, indicating that LCF granule alleviated the renal injury of MRL/lpr mice. Furthermore, LCF granule decreased p65 NF-κB levels and increased Sirt1 and Nrf2 levels in the kidney tissues of MRL/lpr mice, which might elucidate the beneficial effects of LCF on lupus nephritis. In conclusion, this study demonstrates that LCF granule has therapeutic effects on lupus-prone MRL/lpr mice.

  19. Separation of macromolecular proteins and rejection of toxic heavy metal ions by PEI/cSMM blend UF membranes.

    Science.gov (United States)

    Kanagaraj, P; Nagendran, A; Rana, D; Matsuura, T; Neelakandan, S

    2015-01-01

    The charged surface modifying macromolecule (cSMM) was blended into the casting solution of poly(ether imide) (PEI) to prepare surface modified ultrafiltration membranes by phase inversion technique. The separation of proteins including bovine serum albumin, egg albumin, pepsin and trypsin was investigated by the fabricated membranes. On increasing cSMM content, solute rejection decreases whereas membrane flux increases. The pore size and surface porosity of the 5 wt% cSMM blend PEI membranes increases to 41.4 Å and 14.8%, respectively. Similarly, the molecular weight cut-off of the membranes ranged from 20 to 45 kDa, depending on the various compositions of the prepared membranes. The toxic heavy metal ions Cu(II), Cr(III), Zn(II) and Pb(II) from aqueous solutions were subjected to rejection by the prepared blended membrane with various concentration of polyethyleneimine (PETIM) as water soluble polymeric ligand. It was found that the rejection behavior of metal ion depends on the PETIM concentration and the stability complexation of metal ion with ligand.

  20. Preparation and characterization of polymer blend based on sulfonated poly (ether ether ketone) and polyetherimide (SPEEK/PEI) as proton exchange membranes for fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Hashim, Nordiana; Ali, Ab Malik Marwan [Ionic Material and Devices Research Laboratory, Institute of Science, Universiti Teknologi MARA, 40450 Shah Alam (Malaysia); Lepit, Ajis; Rasmidi, Rosfayanti [Faculty of Applied Sciences, Universiti Teknologi MARA Sabah, Beg Berkunci 71, 88997 Kota Kinabalu (Malaysia); Subban, Ri Hanum Yahaya [Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam (Malaysia); Institute of Science, Universiti Teknologi MARA, 40450 Shah Alam (Malaysia); Yahya, Muhd Zu Azhan [Faculty of Defence Science & Technology, Universiti Pertahanan Nasional Malaysia, 57000 Kuala Lumpur (Malaysia)

    2015-08-28

    Blends of sulfonated poly (ether ether ketone) (SPEEK) and polyetherimide (PEI) were prepared in five different weight ratios using N-methyl-2-pyrrolidone (NMP) as solvent by the solution cast technique. The degree of sulfonation (DS) of the sulfonated PEEK was determined from deuterated dimethyl sulfoxide (DMSO-d{sub 6}) solution of the purified polymer using {sup 1}H NMR method. The properties studied in the present investigation includes conductivity, water uptake, thermal stability and structure analysis of pure SPEEK as well as SPEEK-PEI polymer blend membranes. The experimental results show that the conductivity of the membranes increased with increase in temperature from 30 to 80°C, except for that of pure SPEEK membrane which increased with temperature from 30 to 60°C while its conductivity decreased with increasing temperature from 60 to 80°C. The conductivity of 70wt.%SPEEK-30wt.%PEI blend membrane at 80% relative humidity (RH) is found to be 1.361 × 10{sup −3} Scm{sup −1} at 30°C and 3.383 × 10{sup −3} Scm{sup −1} at 80°C respectively. It was also found that water uptake and thermal stability of the membranes slightly improved upon blending with PEI. Structure analysis was carried out using Fourier Transform Infrared (FTIR) spectroscopy which revealed considerable interactions between sulfonic acid group of SPEEK and imide groups of PEI. Modification of SPEEK by blending with PEI shows good potential for improving the electrical and physical properties of proton exchange membranes.

  1. Acute in vivo toxicity mitigation of PEI-coated maghemite nanoparticles using controlled oxidation and surface modifications toward siRNA delivery.

    Science.gov (United States)

    Israel, Liron Limor; Lellouche, Emmanuel; Ostrovsky, Stella; Yarmiayev, Valeria; Bechor, Moshe; Michaeli, Shulamit; Lellouche, Jean-Paul Moshe

    2015-07-22

    A ceric ammonium nitrate (CAN)-based doping step was used for the fabrication of core maghemite nanoparticles (NPs) that enabled the obtainment of colloid particles with a view to a high-level nanoparticle (NP) surface doping by Ce(III/IV). Such doping of Ce(III/IV) cations enables one to exploit their quite rich coordination chemistry for ligand coordinative binding. In fact, they were shown to act as powerful Lewis acid centers for attaching any organic (Lewis base) ligand such as a 25 kDa branched PEI polymer. Resulting conPEI25-CAN-γ-Fe2O3 NPs have been fully characterized before a successful implementation of siRNA loading and cell delivery/gene silencing using a well-known dual luciferase system. This attractive result emphasized their significant potential as an NP platform technology toward additional MRI and/or drug delivery (peptide)-relating end applications. However, due to their high positive charge, PEI polymers can cause severe in vivo toxicity due to their interaction with negatively charged red blood cells (RBC), resulting in RBC aggregation and lysis, leading to thrombosis and, finally, to animal death. In order to mitigate these acute toxic effects, two different types of surface modifications were performed. One modification included the controlled oxidation of 0.1-5% of the PEI amines before or after conjugation to the NPs, using hydrogen peroxide or potassium persulfate. The other type of modification was the addition of a second biocompatible polyanionic polymer to the PEI grafted NPs, based on the concept of a layer-by-layer (LbL) technique. This modification is based on the coordination of another polyanionic polymer on the NPs surface in order to create a combined hybrid PEI and polyanionic polymer nanosystem. In both cases, the surface modification successfully mitigated the NP acute in vivo toxicity, without compromising the silencing efficiency.

  2. Hydrothermal synthesis of nanostructured hybrids based on iron oxide and branched PEI polymers. Influence of high pressure on structure and morphology

    Energy Technology Data Exchange (ETDEWEB)

    Popescu, L.M., E-mail: mpopescu@imnr.ro [National R& D Institute for Non-ferrous and Rare Metals, 102 Biruintei Blvd, Pantelimon, Judetul Ilfov (Romania); Piticescu, R.M., E-mail: roxana@imnr.ro [National R& D Institute for Non-ferrous and Rare Metals, 102 Biruintei Blvd, Pantelimon, Judetul Ilfov (Romania); Petriceanu, M., E-mail: mirelap@imnr.ro [National R& D Institute for Non-ferrous and Rare Metals, 102 Biruintei Blvd, Pantelimon, Judetul Ilfov (Romania); Ottaviani, M.F., E-mail: maria.ottaviani@uniurb.it [University of Urbino “Carlo Bo”, Department of Earth, Life and Environmental Sciences, Urbino (Italy); Cangiotti, M., E-mail: michela.cangiotti@uniurb.it [University of Urbino “Carlo Bo”, Department of Earth, Life and Environmental Sciences, Urbino (Italy); Vasile, E., E-mail: eugeniuvasile@yahoo.com [University Politehnica of Bucharest, Bucharest (Romania); National R& D Institute for Non-ferrous and Rare Metals, 102 Biruintei Blvd, Pantelimon, Judetul Ilfov (Romania); Dîrtu, M.M., E-mail: marinela.dirtu@uclouvain.be [Institute of Condensed Mater and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université Catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve (Belgium); Wolff, M., E-mail: mariusz.wolff@uclouvain.be [Institute of Condensed Mater and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université Catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve (Belgium); Garcia, Y., E-mail: yann.garcia@uclouvain.be [Institute of Condensed Mater and Nanosciences, Molecules, Solids and Reactivity (IMCN/MOST), Université Catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve (Belgium); and others

    2015-07-01

    Homogeneous hybrids in which iron oxide nanoparticles are entrapped within polymer structure are of interest for their potential applications in biomedical field, such as diagnostic, therapeutic and theranostic purposes. For this reason, hybrid nanomaterials based on branched polyethyleneimine (PEI) and iron oxide with different ratios were synthesized in a single step by hydrothermal procedure at high pressure and low temperature. Iron oxide is formed in the presence of branched PEI and the interaction between them takes place in the reaction medium. The influence of synthesis parameters on the hybrid formation, as well as chemical and structural properties was studied by means of FTIR, DSC-TG, HRTEM, electron paramagnetic resonance (EPR), magnetic measurements (SQUID) and {sup 57}Fe Mössbauer analyses. It has been shown that synthesis parameters influence thermal stability and morphology of the hybrids. FeO(OH) crystallites of 2–5 nm are formed. Iron oxyhydroxide nanoparticles strongly entrapped in PEI structure are obtained. The low and distributed values of the specific spontaneous magnetisation in samples prepared under the same pressure conditions support the presence of very fine FeO(OH) nanoparticles, which formation and magnetic properties are depending on the mass ratio between iron oxide and PEI. - Highlights: • Polyethyleneimine (PEI) – iron oxide hybrids were synthesized by hydrothermal method. • Synthesis parameters influence thermal stability and morphology of the hybrids. • Small crystallites of FeO(OH) with size between 2 and 5 nm are formed. • Formation of stable hybrid nanostructures in the pressure range 1000–3000 atm. • FeO(OH) nanoparticles are entrapped in PEI structure at low inorganic–organic ratio.

  3. Qualitative Assessment of the Symptoms and Impact of Pancreatic Exocrine Insufficiency (PEI) to Inform the Development of a Patient-Reported Outcome (PRO) Instrument.

    Science.gov (United States)

    Johnson, Colin D; Arbuckle, Rob; Bonner, Nicola; Connett, Gary; Dominguez-Munoz, Enrique; Levy, Philippe; Staab, Doris; Williamson, Nicola; Lerch, Markus M

    2017-03-22

    Pancreatic exocrine insufficiency (PEI) affects patients with chronic pancreatitis (CP) and cystic fibrosis (CF) who produce insufficient digestive pancreatic enzymes. Common symptoms include steatorrhoea, diarrhea, and abdominal pain. The objective of the study was to develop and test the content validity of a patient-reported outcome (PRO) instrument assessing PEI symptoms and their impact on health-related quality of life. Instrument development was supported by a literature review, expert physician interviews (n = 10: Germany 4, UK 3, France 3), and exploratory, qualitative, concept-elicitation interviews with patients with CF and CP with PEI (n = 61: UK 29, Germany 18, France 14) and expert physicians (n = 10). Cognitive debriefing of the draft instrument was then performed with patients with PEI (n = 37: UK 24, Germany 8, France 5), and feasibility was assessed with physicians (n = 3). For all interviews, verbatim transcripts were qualitatively analysed using thematic analysis methods and Atlas.ti computerized qualitative software. All themes were data driven rather than a priori. Patient interviews elicited symptoms and impacts not reported in the literature. Six symptom concepts emerged: pain, bloating, bowel symptoms, nausea/vomiting, eating problems, and tiredness/fatigue. Six impact domains were also identified. A 45-item instrument was developed in English, French, and German for testing in cognitive debriefing patient interviews. Following cognitive debriefing, 18 items were deleted. Rigorous qualitative patient research and expert clinical input supported development of a PEI-specific PRO with the potential to aid management and monitoring of unmet needs among patients with PEI. The next step is to perform psychometric evaluation of the resulting instrument.

  4. 75 FR 55975 - Safety Zone; San Diego Harbor Shark Fest Swim; San Diego Bay, San Diego, CA

    Science.gov (United States)

    2010-09-15

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego Harbor Shark Fest Swim; San Diego Bay, San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone upon the navigable waters of the San Diego Bay, San Diego, CA, in support...

  5. Internal Causes and Reflections on Wretchedness of Female Existence--Comment on Feng Shui by Fang Fang%世俗环境中的痛楚阴影与美丽光芒——影片万箭穿心的女性主义阐释

    Institute of Scientific and Technical Information of China (English)

    李静

    2012-01-01

    Based on an ordinary female worker Li Baoli's marriage tragedy, Fang Fang describes her suffering, reveals the internal causes of wretchedness of modem female existence, and reflects how women maintain self-value and dignity in their marriages and family lives in her novel Feng Shui.%方方《万箭穿心》围绕普通女工李宝莉的婚姻悲剧,道尽了其处于生活中的种种痛苦。作者以冷峻的笔调揭露了现代女性生存之痛的内在原因,同时也反思女性在婚姻家庭生活中如何保持自我价值和尊严。

  6. Structure and mechanics of the San Andreas-San Gregorio fault junction, San Francisco, California

    Science.gov (United States)

    Parsons, Tom; Bruns, Terry R.; Sliter, Ray

    2005-01-01

    The right-lateral San Gregorio and San Andreas faults meet west of the Golden Gate near San Francisco. Coincident seismic reflection and refraction profiling across the San Gregorio and San Andreas faults south of their junction shows the crust between them to have formed shallow extensional basins that are dissected by parallel strike-slip faults. We employ a regional finite element model to investigate the long-term consequences of the fault geometry. Over the course of 2-3 m.y. of slip on the San Andreas-San Gregorio fault system, elongated extensional basins are predicted to form between the two faults. An additional consequence of the fault geometry is that the San Andreas fault is expected to have migrated eastward relative to the San Gregorio fault. We thus propose a model of eastward stepping right-lateral fault formation to explain the observed multiple fault strands and depositional basins. The current manifestation of this process might be the observed transfer of slip from the San Andreas fault east to the Golden Gate fault.

  7. 方剑乔教授治疗肩周炎经验总结%Pro. Fang Jianqiao's Experience in Treating Frozen Shoulder

    Institute of Scientific and Technical Information of China (English)

    陈敏之; 尹小虎; 方剑乔

    2014-01-01

    [目的]总结方剑乔教授运用经皮穴位神经电刺激治疗肩周炎的临床经验。[方法]通过收集临床病例资料、记录方剑乔教授的临证经验,对方剑乔教授使用经皮穴位神经电刺激配合温针灸治疗肩周炎的方法进行整理与分析。[结果]方剑乔教授认为肩周炎主要是由无菌性炎症引起的。在诊断上重视临床体格检查;治疗上使用经皮穴位神经电刺激,配合局部阿是穴温针灸以疏通经络,行气活血止痛,疗效显著。[结论]方剑乔教授运用经皮穴位神经电刺激疗法治疗肩周炎,临床疗效显著,为治疗肩周炎提供了一种无创伤性、经济安全,操作简便且有效的治疗方法,临床上值得大力推广。%[Objective] To summarize the clinical experience on treating frozen shoulder by using transcutaneous electrical nerve stimulation, TENS. [Method] By col ecting clinical cases and record of Pro. Fang Jianqiao clinical experience to sort and analyze the methodology of Pro. Fang Jianqiao's clinical y used TENS and warm acupuncture to treatment frozen shoulder. [Result] Professor Fang Jianqiao applies the theory of anatomy and muscle along meridians in his diagnosis and treatment on frozen shoulder, which is based on its origin theory of aseptic inflammation. Treatment with transcutaneous electrical nerve stimulation, TENS, combined with local Ashi warm acupuncture to clear the meridians, qi and blood circulation and relieve pain. [conclusion] Professor Fang Jianqiao has great innovation on treatment of frozen shoulder and the combination of Transcutaneous electrical nerve stimulation, TENS on frozen shoulder has noninvasive, economy, safe and significant effect in clinic. This clinical method is worthy promotion to public.

  8. A Doctoral Dissertation by Undesirable Learning Style——On Liu Fang's Song Dynasty Double Capital of Urban Culture and Literary Production

    Institute of Scientific and Technical Information of China (English)

    Tan Qingyang

    2011-01-01

    Recently,I saw a doctoral dissertation online.It is Liu Fang' s Song Dynasty Double Capital of Urban Culture and Literary Production which passed Ph.D.thesis defense in a university in 2008.At the first glance at the subject,it is very attractive.While I read the afterword and knew the topic had won three funds given by his university,his province and the Ministry of Education respectively,it seemed even better.But after carefully reading the text,I was disappointed.We can say that it is not only filled with faults everywhere,but also by undesirable learning style.

  9. [The classification method of syndromes in the Bei ji qian jin yao fang (Essential Recipes for Emergent Use Worth A Thousand Gold)].

    Science.gov (United States)

    Shen, Xiaowei; Zheng, Wenjie; Ma, Mingyue; Zhang, Zhibin

    2014-03-01

    Bei ji qian jin yao fang (Essential Recipes for Emergent Use Worth A Thousand Gold) epitomizes the medical achievements of the Tang Dynasty with an extensive coverage. The classification method of syndromes in this book is derived from Nei jing (Inner Canon), Nan jing (Classic of Questioning), Shang han za bing lun (Treatise on Cold Pathogenic and Miscellaneous Diseases), Zhu bing yuan hou lun (General Treatise on Causes and Manifestations of All Diseases), and enlightens the later generation with many unique characteristics, reflecting the mature recognition of the diseases in the contemporary clinical medicine from one side. It is still of great significance for understanding the diseases and exploring its pathogenesis even today.

  10. Influência da temperatura no desempenho mecânico de compósitos PEI/fibras de vidro Influence from the temperature on the mechanical behavior of PEI/glass fiber composites

    Directory of Open Access Journals (Sweden)

    Gustavo H. Oliveira

    2009-01-01

    Full Text Available Os compósitos poliméricos vêm ganhando grande destaque devido aos avanços tecnológicos conquistados pelas indústrias, principalmente a aeroespacial. Estes avanços têm como uma de várias finalidades a obtenção de compósitos com baixa massa específica associada às elevadas resistência mecânica e rigidez. Comparando os compósitos termoplásticos aos termorrígidos, pode ser destacado como suas principais vantagens: processamento mais rápido pela ausência de um ciclo de cura; baixa absorção de umidade; excelente resistência química; maior temperatura de serviço; baixo custo de transporte e estocagem; maior resistência ao impacto; maior rigidez e a possibilidade de serem reciclados. Devido às grandes variações de temperatura que estes materiais estão sujeitos durante sua vida útil como artefatos de aplicação aeronáutica, é de extrema importância a realização de estudos do seu comportamento mecânico a elevadas temperaturas, uma vez que a estas temperaturas as propriedades do compósito podem ser modificadas. O presente trabalho visa avaliar o comportamento mecânico (tração, fadiga e cisalhamento interlaminar, tanto em temperatura ambiente como a 80 °C de compósitos termoplásticos de matriz PEI (poli(éter-imida reforçados com fibras de vidro. Observou-se que os valores de resistências à tração, cisalhamento e fadiga do laminado PEI/fibras de vidro foram superiores aos encontrados para o laminado de epóxi/fibras de vidro. Entretanto, em todos os casos foram observadas quedas significativas nas propriedades mecânicas obtidas a 80 °C, em comparação com aquelas à temperatura ambiente.Polymeric composites have been used due to the technological progress in industries, mainly the aerospace. These advances are aimed at obtaining composites with low specific gravity associated with high mechanical properties and stiffness. The main advantages of thermoplastics over thermoset laminates include: fast

  11. Una aportación al estudio del cuento fang de Guinea Ecuatorial en lengua española / A CONTRIBUTION TO THE STUDY ABOUT THE FANG TALES FROM EQUATORIAL GUINEA IN SPANISH LANGUAGE

    Directory of Open Access Journals (Sweden)

    María Teresa Abaga Envó

    2009-06-01

    Full Text Available Resumen: Una lectura sobre los estudios narratológicos revela que el acto narrativo es un hecho consustancial al ser humano. “Somos el homo sapiens, porque somos el homo narrans”, diría el académico José María Merino (2002: 57. De las estructuras narrativas, el cuento oral tradicional se alza como la forma más antigua. Tan antigua y presente en todos los pueblos que componentes semánticos y formales se repiten en relatos de distintas culturas. Tales aspectos constituyen el objeto de análisis de este repertorio de cuentos fantásticos de Guinea Ecuatorial transmitidos en lengua española. En dicho entorno, el cuento oral fang se manifiesta como un arte viviente. No es un evento pensado sólo para niños; toda la comunidad participa de esta “fiesta” que se verá enaltecida por la presencia del canto. Dada la escasa proliferación de estudios del relato oral africano y en particular del guineano, se hace imperativo partir de estudios occidentales, lo cual nos desliza hacia el terreno del comparativismo. Sin embargo, no siempre dichos estudios son confirmados en esta investigación que concluye con las siguientes tesis: el criterio verdad y ficción al que se recurre para delimitar ciertas narraciones tradicionales europeas queda difuminado; uso irregular de las fórmulas de abertura y cierre; la asignación del fin lúdico de los cuentos fantásticos se acompaña del didáctico y la pretendida universalización del método estructuralista formal de Vladimir Propp, expuesta en Morfología del cuento (1987, se vislumbra inoperante, pues quedan reducidas las treinta y una funciones y la sucesión lineal de las mismas. Los temas y los motivos, arropados por el espíritu de la cultura donde emergen, son similares a los identificados en compilaciones españolas o internacionales. Finalmente, la naturaleza oral tradicional del repertorio queda patente a través de las nueve “Leyes Épicas” establecidas por Axel Orlik (1965: la Ley de

  12. Regulating effect of TongXie-YaoFang on colonic epithelial secretion via Cl- and HCO3- channel

    Science.gov (United States)

    Yang, Cheng; Xiong, Ying; Zhang, Sheng-Sheng; An, Fang-Mei; Sun, Jing; Zhang, Qing-Lin; Zhan, Qiang

    2016-01-01

    AIM To investigate the pharmacological effect of TongXie-YaoFang (TXYF) formula, a Chinese herbal formula, on Diarrhea-predominant irritable bowel syndrome (D-IBS) rats. METHODS In a neonatal maternal separation plus restraint stress (NMS + RS) model of D-IBS, male Sprague Dawley rats were randomly divided into two groups (NMS + RS group and TXYF-formula group) with no handlings were used as controls (NH group). Starting from postnatal day 60, rats in TXYF-formula group were administered TXYF-formula (4.92 g/100 g bodyweight) orally twice a day for 14 consecutive days while NH group and NMS + RS group were given distilled water. Using short-circuit current technology, we observed 5-HT-induced changes of current across ion channels, such as cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, epithelial Na+ channel (ENaC), Ca2+-dependent Cl- channel (CACC), Na+-K+-2Cl- co-transporter (NKCC), and Na+-HCO3- co-transporter (NBC), in the colonic epithelium of three groups after exposure to drugs and specific blockers with a Power Lab System (AD Instruments International). RESULTS Under basal conditions, the changes of short-circuit current (∆Isc, µA/cm2) induced by 5-HT were similar in NH group and TXYF-formula group, and both higher than NMS + RS group (70.86 µA/cm2 ± 12.32 µA/cm2, 67.67 µA/cm2 ± 11.68 µA/cm2 vs 38.8 µA/cm2 ± 7.25 µA/cm2, P CFTR Cl- channel was blocked by 1,1-dimethyl piperidinium chloride (DPC), 5-HT-induced ∆Isc did not significantly differ in three groups (42.28 µA/cm2 ± 10.61 µA/cm2 vs 51.48 µA/cm2 ± 6.56 µA/cm2 vs 47.75 µA/cm2 ± 7.99 µA/cm2, P > 0.05, respectively). The similar results could also be obtained in three groups when NBC and NKCC were respectively blocked by their blockers. CONCLUSION TXYF-formula can regulate the Cl- and HCO3- secretion of colonic mucosa via CFTR Cl- channel, Cl-/HCO3- exchanger, NBC and NKCC co-transporters. PMID:28082810

  13. Ergonomic Analysis of UI’s BicyclesUsing Posture Evaluation Index (PEI Method in Virtual Environment

    Directory of Open Access Journals (Sweden)

    Erlinda Muslim

    2010-10-01

    Full Text Available This research was conducted to study ergonomic aspect from University of Indonesia bicycle in virtual environment. Software Jack 6.0 was used to analyze it. PEI was used as approach that integrated the results of three methods: Lower Back Analysis, Ovako Working Posture Analysis, and Rapid Upper Limb Assessment. The research objective is to evaluate existing design of University of Indonesia bicycle and to determine the most ergonomic redesign which concern with handlebar height and saddle height modification. The result showed that the most ergonomic design of University of Indonesia bicycle is the one with the highest handlebar height (22 cm and the lowest saddle height (11 cm.

  14. Involvement of canine oral papillomavirus in generalized oral and cutaneous verrucosis in a Chinese Shar Pei dog.

    Science.gov (United States)

    Sundberg, J P; Smith, E K; Herron, A J; Jenson, A B; Burk, R D; Van Ranst, M

    1994-03-01

    Severe papillomatosis developed in the oral cavity and spread throughout the haired skin of the trunk and limbs of an 8-month-old female Chinese Shar Pei dog. The dog had received corticosteroids prior to referral, which was associated with the onset of demodecosis and papillomatosis. Papillomavirus structural antigens were detected in biopsies by immunohistochemistry using a panel of monoclonal and polyclonal antibodies. An 8.2-kilobase papillomavirus-specific DNA molecule was detected in the cutaneous lesions by high stringency Southern blot hybridization using a cloned canine oral papillomavirus DNA probe. Restriction enzyme analysis revealed that the virus in the cutaneous lesions was identical to the canine oral papillomavirus. Discontinuation of the steroids combined with the use of a mitocide, antibiotics, and an autogenous vaccine resolved the demodecosis and papillomatosis. This case report suggests that corticosteroid-induced immunosuppression can expand the tissue tropism of papillomaviruses.

  15. Two-Sided Surface Oxidized Cellulose Membranes Modified with PEI: Preparation, Characterization and Application for Dyes Removal

    Directory of Open Access Journals (Sweden)

    Wei Wang

    2017-09-01

    Full Text Available Porous regenerated cellulose (RC membranes were prepared with cotton linter pulp as a raw material. These membranes were first oxidized on both sides by a modified (2,2,6,6-tetramethylpiperidin-1-yloxyl (TEMPO oxidation system using a controlled oxidation reaction technique. Then, the oxidized RC membranes were functionalized with polyethylenimine (PEI via the glutaraldehyde crosslinking method to obtain bifunctional (carboxyl and amino porous RC membranes, as revealed by Fourier transform infrared spectroscopy (FT-IR, elemental analysis and zeta potential measurement. The scanning electron microscopy (SEM and the tests of the mechanical properties and permeability characteristics of modified RC membranes demonstrated that the porous structure and certain mechanical properties could be retained. The adsorption performance of the modified membranes towards dyes was subsequently investigated. The modified membranes displayed good adsorption capacities, rapid adsorption equilibrium and removal efficiencies towards both anionic (xylenol orange (XO and cationic (methylene blue (MB dyes, making them suitable bioadsorbents for wastewater treatment.

  16. O ENVELHECIMENTO, A DOENÇA DE ALZHEIMER E AS CONTRIBUIÇÕES DO PROGRAMA DE ENRIQUECIMENTO INSTRUMENTAL (PEI

    Directory of Open Access Journals (Sweden)

    Antonia Rozeli Roberto de Oliveira

    2010-06-01

    Full Text Available This article suggests that the aging process has unique and distinct features. A distinction is made between normal and pathological aging. Actually we are concerned that a successful aging is in close relationship with the pre-vention of diseases, and maintenance of physical and cognitive activities. Epidemiological studies show that elderly people with mild cognitive deficts are at greater risk of developing Alzheimer's disease. This disease affects brain function, primarily memory, and also speech, executive functions, praxias and gnosias. Besides the current pharmacological approach, alterna-tive therapies, like neuropsychological rehabilitation, are in current use and contributes in a great amount to the improvement of cognitive and behav-ioral functions. In this article we gave special attention using PEI and EAM, in neuropsychological rehabilitation programs directed to Alzheimer`s dis-ease, focusing the improvement of residual functions and aiming the reduc-tion of the impact that this disease brings to the quality of life.

  17. Nano-TiO2 reinforced PEEK/PEI blends as biomaterials for load-bearing implant applications.

    Science.gov (United States)

    Díez-Pascual, Ana M; Díez-Vicente, Angel L

    2015-03-11

    Biocompatible ternary nanocomposites based on poly(ether ether ketone) (PEEK)/poly(ether imide) (PEI) blends reinforced with bioactive titanium dioxide (TiO2) nanoparticles were fabricated via ultrasonication followed by melt-blending. The developed biomaterials were characterized using FT-IR, SEM, XRD, DSC, TGA, and DMA. Further, their water-absorption, tensile, tribological, dielectric, and antibacterial properties were evaluated. PEI acts as a coupling agent, since it can interact both with PEEK via π-π stacking and polar interactions as well as with the nanoparticles through hydrogen bonding, as corroborated by the FT-IR spectra, which resulted in a homogeneous titania dispersion within the biopolymer blend without applying any particle surface treatment or polymer functionalization. A change from promotion to retardation in the crystallization rate of the matrix was found with increasing TiO2 concentration, while its crystalline structure remained unaltered. The nanoparticles stiffened, strengthened, and toughened the matrix simultaneously, and the optimal properties were achieved at 4.0 wt % TiO2. More interesting, the tensile properties were retained after steam sterilization in an autoclave or exposure to a simulated body fluid (SBF). The nanocomposites also displayed reduced water absorption though higher thermal stability, storage modulus, glass transition temperature, dielectric constant, and dielectric loss compared to the control blend. Further, remarkable enhancements in the tribological properties under both SBF and dry environments were attained. The nanoparticles conferred antibacterial action versus Gram-positive and Gram-negative bacteria in the presence and the absence of UV light, and the highest inhibition was attained at 4.0 wt % nanoparticle concentration. These nanocomposites are expected to be used in long-term load-bearing implant applications.

  18. San Cástulo

    OpenAIRE

    Jaramillo, Tania

    2014-01-01

    Porque no te acercas y nos entendemos, nos vamos cayendo por el lucro de la colonia, nos perdemos en la esquina de san Cástulo y nos vamos volando a Eleuterio, en una noche, que la luna nos vigile, que nos aguarde, que retrase el día, y la gente permanezca dormida o despierta pero temerosa de la noche, de los policías y los delincuentes, de los violadores y de nosotros, de la vida nocturna, de ese lugar oscuro en alguna parte, donde nos convertimos y aullamos.

  19. San Cástulo

    OpenAIRE

    Jaramillo, Tania

    2014-01-01

    Porque no te acercas y nos entendemos, nos vamos cayendo por el lucro de la colonia, nos perdemos en la esquina de san Cástulo y nos vamos volando a Eleuterio, en una noche, que la luna nos vigile, que nos aguarde, que retrase el día, y la gente permanezca dormida o despierta pero temerosa de la noche, de los policías y los delincuentes, de los violadores y de nosotros, de la vida nocturna, de ese lugar oscuro en alguna parte, donde nos convertimos y aullamos.

  20. Coma blisters sans coma.

    Science.gov (United States)

    Heinisch, Silke; Loosemore, Michael; Cusack, Carrie A; Allen, Herbert B

    2012-09-01

    Coma blisters (CBs) are self-limited lesions that occur in regions of pressure during unconscious states classically induced by barbiturates. We report a case of CBs sans coma that were histologically confirmed in a 41-year-old woman who developed multiple tense abdominal bullae with surrounding erythema following a transatlantic flight. Interestingly, the patient was fully conscious and denied medication use or history of medical conditions. A clinical diagnosis of CBs was confirmed by histopathologic findings of eccrine gland necrosis, a hallmark of these bulIous lesions.

  1. Fabrication of Core-Shell PEI/pBMP2-PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells

    Directory of Open Access Journals (Sweden)

    Qiao Xie

    2016-01-01

    Full Text Available Bone tissue engineering is the most promising technology for enhancing bone regeneration. Scaffolds loaded with osteogenic factors improve the therapeutic effect. In this study, the bioactive PEI (polyethylenimine/pBMP2- (bone morphogenetic protein-2 plasmid- PLGA (poly(D, L-lactic-co-glycolic acid core-shell scaffolds were prepared using coaxial electrospinning for a controlled gene delivery to hPDLSCs (human periodontal ligament stem cells. The pBMP2 was encapsulated in the PEI phase as a core and PLGA was employed to control pBMP2 release as a shell. First, the scaffold characterization and mechanical properties were evaluated. Then the gene release behavior was analyzed. Our results showed that pBMP2 was released at high levels in the first few days, with a continuous release behavior in the next 28 days. At the same time, PEI/pBMP2 showed high transfection efficiency. Moreover, the core-shell electrospun scaffold showed BMP2 expression for a much longer time (more than 28 days compared with the single axial electrospun scaffold, as evaluated by qRT-PCR and western blot after culturing with hPDLSCs. These results suggested that the core-shell PEI/pBMP2-PLGA scaffold fabricated by coaxial electrospinning had a good gene release behavior and showed a prolonged expression time with a high transfection efficiency.

  2. Elucidating the interplay between DNA-condensing and free polycations in gene transfection through a mechanistic study of linear and branched PEI

    DEFF Research Database (Denmark)

    Dai, Zhuojun; Gjetting, Torben; Mattebjerg, Maria Ahlm;

    2011-01-01

    In the present study we compare LPEI and BPEI characteristics related to DNA condensation and their role as free polycation chains in gene transfection. Using radioactive 32P labeled DNA, we investigated the effect of free PEI chains on the cellular uptake of polyplexes. Our investigations show d...

  3. Co-delivery of Doxorubicin Encapsulated PLGA Nanoparticles and Bcl-xL shRNA Using Alkyl-Modified PEI into Breast Cancer Cells.

    Science.gov (United States)

    Ebrahimian, Mahboubeh; Taghavi, Sahar; Mokhtarzadeh, Ahad; Ramezani, Mohammad; Hashemi, Maryam

    2017-02-24

    In recent years, much effort has been focused on an appropriate combination of chemotherapeutic drugs and nucleic acids to exploit additive or synergistic therapeutic effects and overcome many obstacles such as the reduction of side effects and drug resistance. Short hairpin RNA (shRNA) has designed to allow the production of small interfering RNA (siRNA) within the cells and offer long-lasting silencing of target genes. In this study, alkyl-modified polyethylenimine (PEI 10 kD) was used for co-delivery of doxorubicin (DOX) encapsulated into poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) and Bcl-xL shRNA (one class of molecules that block apoptosis of tumor cells) into breast cancer cells. Our results demonstrated that modification of PEI with alkyl chain could enhance the induction of apoptosis in tumor cells by suppression of Bcl-xL gene using Bcl-xL shRNA more than PEI alone. On the other hand, DOX encapsulated into PLGA had more synergistic effect with shRNA in comparison with DOX alone. In conclusion, combination of PLGA-DOX NPs and alkyl-PEI/shRNA complexes may have promising applications in breast cancer therapy.

  4. San Diego's Capital Planning Process

    Science.gov (United States)

    Lytton, Michael

    2009-01-01

    This article describes San Diego's capital planning process. As part of its capital planning process, the San Diego Unified School District has developed a systematic analysis of functional quality at each of its school sites. The advantage of this approach is that it seeks to develop and apply quantifiable metrics and standards for the more…

  5. Los Angeles og San Francisco

    DEFF Research Database (Denmark)

    Ørstrup, Finn Rude

    1998-01-01

    Kompendium udarbejdet til en studierejse til Los Angeles og San Francisco april-maj 1998 Kunstakademiets Arkitektskole, Institut 3H......Kompendium udarbejdet til en studierejse til Los Angeles og San Francisco april-maj 1998 Kunstakademiets Arkitektskole, Institut 3H...

  6. A novel unstable duplication upstream of HAS2 predisposes to a breed-defining skin phenotype and a periodic fever syndrome in Chinese Shar-Pei dogs.

    Directory of Open Access Journals (Sweden)

    Mia Olsson

    2011-03-01

    Full Text Available Hereditary periodic fever syndromes are characterized by recurrent episodes of fever and inflammation with no known pathogenic or autoimmune cause. In humans, several genes have been implicated in this group of diseases, but the majority of cases remain unexplained. A similar periodic fever syndrome is relatively frequent in the Chinese Shar-Pei breed of dogs. In the western world, Shar-Pei have been strongly selected for a distinctive thick and heavily folded skin. In this study, a mutation affecting both these traits was identified. Using genome-wide SNP analysis of Shar-Pei and other breeds, the strongest signal of a breed-specific selective sweep was located on chromosome 13. The same region also harbored the strongest genome-wide association (GWA signal for susceptibility to the periodic fever syndrome (p(raw = 2.3 × 10⁻⁶, p(genome = 0.01. Dense targeted resequencing revealed two partially overlapping duplications, 14.3 Kb and 16.1 Kb in size, unique to Shar-Pei and upstream of the Hyaluronic Acid Synthase 2 (HAS2 gene. HAS2 encodes the rate-limiting enzyme synthesizing hyaluronan (HA, a major component of the skin. HA is up-regulated and accumulates in the thickened skin of Shar-Pei. A high copy number of the 16.1 Kb duplication was associated with an increased expression of HAS2 as well as the periodic fever syndrome (p < 0.0001. When fragmented, HA can act as a trigger of the innate immune system and stimulate sterile fever and inflammation. The strong selection for the skin phenotype therefore appears to enrich for a pleiotropic mutation predisposing these dogs to a periodic fever syndrome. The identification of HA as a major risk factor for this canine disease raises the potential of this glycosaminoglycan as a risk factor for human periodic fevers and as an important driver of chronic inflammation.

  7. Flow injection chemiluminescence immunoassay of microcystin-LR by using PEI-modified magnetic beads as capturer and HRP-functionalized silica nanoparticles as signal amplifier.

    Science.gov (United States)

    Lu, Jusheng; Wei, Wei; Yin, Lihong; Pu, Yuepu; Liu, Songqin

    2013-03-07

    A rapid sandwiched immunoassay of microcystin-LR (MC-LR) in water is proposed with flow injection chemiluminescence detection. The magnetic beads (MBs) were first modified with polyethyleneimine (PEI) by acylamide bond between the carboxyl group on the surface of MBs and the primary amine group in PEI, followed by immobilizing of anti-MC-LR (Ab1) onto PEI with glutaraldehyde as linkage. The resulting Ab1 modified MBs captured the target MC-LR in water, reacted with the horseradish peroxidase and anti-MC-LR co-immobilized silica nanoparticles, and were detected with flow injection chemiluminescence. When using PEI/MBs as the carrier of anti-MC-LR, the CL signal was greatly enhanced up to 9-fold compared to that using MBs without PEI modification. The CL signal was further amplified 13-fold when Si/Ab2 was used as the signal probe. Under the optimal conditions, the present immunoassay exhibited a wide quantitative range from 0.02 to 200 μg L(-1) with a detection limit of 0.006 μg L(-1), which was much lower than the WHO provisional guideline limit of 1.0 μg L(-1) for MC-LR in drinking water. The relative standard deviation was 4.8% and the recoveries for the spiked samples ranged from 84% to 115%, which indicated acceptable precision and accuracy for MC-LR. The present method is easier to perform and less time-consuming (the entire analysis process lasted about 40 minutes) and has been applied to the detection of MC-LR in different water samples successfully.

  8. Effect of miR-146a/bFGF/PEG-PEI Nanoparticles on Inflammation Response and Tissue Regeneration of Human Dental Pulp Cells

    Directory of Open Access Journals (Sweden)

    Lu Liu

    2016-01-01

    Full Text Available Introduction. Inflammation in dental pulp cells (DPCs initiated by Lipopolysaccharide (LPS results in dental pulp necrosis. So far, whether there is a common system regulating inflammation response and tissue regeneration remains unknown. miR-146a is closely related to inflammation. Basic fibroblast growth factor (bFGF is an important regulator for differentiation. Methods. To explore the effect of miR-146a/bFGF on inflammation and tissue regeneration, polyethylene glycol-polyethyleneimine (PEG-PEI was synthesized, and physical characteristics were analyzed by dynamic light scattering and gel retardation analysis. Cell absorption, transfection efficiency, and cytotoxicity were assessed. Alginate gel was combined with miR-146a/PEG-PEI nanoparticles and bFGF. Drug release ratio was measured by ultraviolet spectrophotography. Proliferation and odontogenic differentiation of DPCs with 1 μg/mL LPS treatment were determined. Results. PEG-PEI prepared at N/P 2 showed complete gel retardation and smallest particle size and zeta potential. Transfection efficiency of PEG-PEI was higher than lipo2000. Cell viability decreased as N/P ratio increased. Drug release rate amounted to 70% at the first 12 h and then maintained slow release afterwards. Proliferation and differentiation decreased in DPCs with LPS treatment, whereas they increased in miR-146a/bFGF gel group. Conclusions. PEG-PEI is a promising vector for gene therapy. miR-146a and bFGF play critical roles in inflammation response and tissue regeneration of DPCs.

  9. Effect of Wuzi Yanzong Fang Against Cyclophosphamide Induced DNA Damage in Mice%五子衍宗方对环磷酰胺致小鼠DNA损伤的影响

    Institute of Scientific and Technical Information of China (English)

    刘苗苗; 袁丁; 黄威峰; 彭奔; 张长城

    2014-01-01

    Objective To study protective effects of Wuzi Yanzong Fang on DNA damage induced by cyclophosphamide (CTX) in mice, and explore its mechanism. Methods BalB/c mice were randomly divided into normal group, model group, Wuzi Yanzong Fang low dose group and Wuzi Yanzong Fang high dose group. Mice in Wuzi Yanzong Fang groups were pretreated with Wuzi Yanzong Fang for 7 days, then the mice in Wuzi Yanzong Fang groups and model group were intraperitoneally injected with CTX (100 mg/kg) every other day for three times, and mice in Wuzi Yanzong Fang groups were continued administered with Wuzi Yanzong Fang. Animals were sacrificed in twelve hours after the final treatment of CTX. ELISA was used to detect 8-OHdG content in serum, and single cell gel electrophoresis to detect DNA damage in bone marrow cells. Results Wuzi Yanzong Fang low dose group and high dose group reduced the level of 8-OHdG in serum. Wuzi Yanzong Fang significantly decreased Olive tail moment, tail moment, tail length and tail DNA%in mouse bone marrow cells. Conclusion Wuzi Yanzong Fang has good protective effects on DNA damage caused by CTX.%目的:观察五子衍宗方对环磷酰胺(CTX)致小鼠 DNA 损伤的影响,并探讨其作用机制。方法将BalB/c 小鼠随机分为正常组、模型组、五子衍宗方低剂量组、五子衍宗方高剂量组。五子衍宗方低、高剂量组预给药1周后,除正常组外,其余各组腹腔注射CTX 100 mg/kg造模,隔日1次,共3次,同时五子衍宗方低、高剂量组继续给药,末次给予CTX后12 h处死小鼠,分离血清,ELISA检测血清中8-羟基脱氧鸟苷(8-OHdG)的含量,应用单细胞凝胶电泳技术检测骨髓细胞DNA损伤。结果五子衍宗方低、高剂量组能够降低血清中8-OHdG的含量,使小鼠骨髓细胞彗星图像的Olive尾矩、尾距、尾长和尾部DNA含量降低。结论五子衍宗方对CTX致小鼠DNA损伤具有较好的保护作用。

  10. 方北方作品中的人道主义情怀论析%An Analysis of Humanism in Fang Beifang's Works

    Institute of Scientific and Technical Information of China (English)

    金进

    2011-01-01

    方北方的童年和青年时期都在中国度过,特别是1937—1947的十年岁月,在这十年的求学生涯里,已经进入成熟期的中国现代文学对方北方的影响是巨大的,他的长篇代表作“风云三部曲”彰显着他文脉中的中国(文学)经验。而另一部长篇代表作“马来亚三部曲”是方北方反映新马华人由移民心态转向本土认同的重要代表作,他以个人影射历史,为马华现实主义文学打下了重要基础。在近七十的文学生涯中,执守于马来西亚认同的方北方,关心着马华社会成长过程中的种种问题,以自己蓬勃的创作力、饱满的激情,倾诉着他对新马社会的关心,这种人道主义情怀是他留给马华文坛的重要文学精神。%Fang Beifang spent his childhood and youth in China, especially the decade between 1937 and 1947. During those ten years while he was still studying, modem Chinese literature that had matured had an enormous impact on Fang Beifang. His novel masterpiece "The Storm Trilogy" revealed the Chinese (literary) experience in his work. Another novel masterpiece "The Malayan Trilogy" is an important work in which he reflected how the mentality of the Chinese in Singapore and Malaysia had shifted from an immigrant mindset to a recognition of themselves as locals. His technique of using a character to allude to history has laid an important foundation in Mabua realism literature. In his nearly seventy years of literary career, Fang Beifang, who steadfastly stood his ground and held on to his Malaysian identify, was concerned about all the problems in the growth of the Mahua community. He poured out his concerns for the communities in Singapore and Malaysia through his vibrant creativity with full passion. This humanism is an important literary spirit that he has left for the Mabua writing circle.

  11. 方北方小说与马来西亚华人的家国认同%The Malaysian Chinese Homeland Consciousness and Fang Beifang’s Novels

    Institute of Scientific and Technical Information of China (English)

    潘碧华; 祝家丰

    2011-01-01

    本文主要探讨方北方小说在马来西亚华文文学本土化的过程中,对家国意识的转变。华人最初的意识是侨民心态,接着意识到本土化的重要性,国家独立后萌生爱国意识。方北方是马来西亚当代出色的华文作家,他的作品多以历史事实作为背景,穿插个人经历,反映出时代的面貌。方北方重要作品是"风云三部曲"和"激流三部曲"6部长篇小说中。"风云三步曲"以中国抗战为背景,"激流三部曲"则是他在马来(西)亚生活的实际经历的总结。方北方小说记录了华人在马来西亚开垦拓荒的功绩,他的小说也见证了马来(西)亚独立前后华人对家国认同的心理挣扎,与政治背景有着极大的关系。%This article will discuss the transformation of homeland consciousness in Fang Beifang's novels against the background of the gradual localization of Malaysian Chinese literature.Begining with an immigrant identity in the early days,the Chinese gradually realized the importance of localization,and a patriotic consciousness emerged after Malaysia attained its independence.A prominent Malaysian Chinese author Fang Beifang based his works mainly on historical events,enriched with his personal experience and the flavor of the period.Fang Beifang's major works include The Trilogy of Wind and Cloud and The Trilogy of Torrent.The former is set during the anti-Japanese war in China,whereas the latter sums up his real life experiences in Malaya/Malaysia.His works record the Chinese's contribution to the development of Malaysia,as well as the national identity and political dilemmas faced by the Malaysian Chinese community.

  12. Polylysine-modified polyethylenimine (PEI-PLL) mediated VEGF gene delivery protects dopaminergic neurons in cell culture and in rat models of Parkinson's Disease (PD).

    Science.gov (United States)

    Sheikh, Muhammad Abid; Malik, Yousra Saeed; Xing, Zhenkai; Guo, Zhaopei; Tian, Huayu; Zhu, Xiaojuan; Chen, Xuesi

    2017-05-01

    Parkinson's Disease (PD) is a chronic neurodegenerative disorder characterized by motor deficits which result from the progressive loss of dopaminergic neurons. Gene therapy using growth factors such as VEGF seems to be a viable approach for potential therapeutic treatment of PD. In this study, we utilized a novel non-viral gene carrier designated as PEI-PLL synthesized by our laboratory to deliver VEGF gene to study its effect by using both cell culture as well as animal models of PD. For cell culture experiments, we utilized 6-hydroxydopamine (6-OHDA) mediated cell death model of MN9D cells following transfection with either a control plasmid or VEGF expressing plasmid. As compared to control transfected cells, PEI-PLL mediated VEGF gene delivery to MN9D cells resulted in increased cell viability, increase in the number of Tyrosine hydroxylase (TH) positive cells and decreased apoptosis following 6-OHDA insult. Next, we studied the therapeutic potential of PEI-PLL mediated VEGF gene delivery in SNPc by using unilateral 6-OHDA Medial forebrain bundle (MFB) lesion model of PD in rats. VEGF administration prevented the loss of motor functions induced by 6-OHDA as determined by behavior analysis. Similarly, VEGF inhibited the 6-OHDA mediated loss of DA neurons in Substantia Nigra Pars Compacta (SNPc) as well as DA nerve fibers in striatum as determined by TH immunostaining. In addition, PEI-PLL mediated VEGF gene delivery also prevented apoptosis and microglial activation in PD rat models. Together, these results clearly demonstrated the beneficial effects of PEI-PLL mediated VEGF gene delivery on dopaminergic system in both cell culture and animal models of PD. In this report, we exploited the potential of PEI-PLL to deliver VEGF gene for the potential therapeutic treatment of PD by using both cell culture and animal models of PD. To the best of our knowledge, this is the first report describing the use of novel polymeric gene carriers for the delivery of VEGF gene

  13. 78 FR 39610 - Safety Zone; Big Bay Boom, San Diego Bay; San Diego, CA

    Science.gov (United States)

    2013-07-02

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Big Bay Boom, San Diego Bay; San Diego, CA... temporary safety zones upon the navigable waters of the San Diego Bay for the annual Port of San Diego... Sector San Diego, Coast Guard; telephone 619-278-7261, email d11marineeventssd@uscg.mil . If you have...

  14. 75 FR 38412 - Safety Zone; San Diego POPS Fireworks, San Diego, CA

    Science.gov (United States)

    2010-07-02

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego POPS Fireworks, San Diego, CA... zone on the ] navigable waters of San Diego Bay in support of the San Diego POPS Fireworks. This safety.... Coast Guard Sector San Diego, CA; telephone 619-278- 7262, e-mail Shane.E.Jackson@uscg.mil . If you have...

  15. 78 FR 42027 - Safety Zone; San Diego Bayfair; Mission Bay, San Diego, CA

    Science.gov (United States)

    2013-07-15

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego Bayfair; Mission Bay, San Diego... proposing a temporary safety zone on the navigable waters of Mission Bay in San Diego, CA for the San Diego..., call or email Lieutenant John Bannon, Waterways Management, U.S. Coast Guard Sector San Diego...

  16. 78 FR 29289 - Safety Zone; Big Bay Boom, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2013-05-20

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Big Bay Boom, San Diego Bay, San Diego, CA... establish four temporary safety zones upon the navigable waters of San Diego ] Bay for the Port of San Diego... Management, U.S. Coast Guard Sector San Diego; telephone (619) 278-7261, email John.E.Bannon@uscg.mil . If...

  17. 78 FR 53245 - Safety Zone; San Diego Bayfair; Mission Bay, San Diego, CA

    Science.gov (United States)

    2013-08-29

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego Bayfair; Mission Bay, San Diego... temporary safety zone upon the navigable waters of Mission Bay in San Diego, CA for the annual San Diego... Management, U.S. Coast Guard Sector San Diego; telephone (619) 278-7261, email John.E.Bannon@uscg.mil . If...

  18. Polyionic complex of single-walled carbon nanotubes and PEG-grafted-hyperbranched polyethyleneimine (PEG-PEI-SWNT) for an improved doxorubicin loading and delivery: development and in vitro characterization.

    Science.gov (United States)

    Farvadi, Fakhrossadat; Tamaddon, AliMohammad; Sobhani, Zahra; Abolmaali, Samira Sadat

    2016-05-13

    To take advantages of single-walled carbon nanotubes (SWNTs) for cellular delivery of chemotherapeutic agents (e.g. doxorubicin) in order to decrease general toxicities of doxorubicin (DOX) and to promote the efficacy, we aimed to develop a novel approach to stabilize SWNTs through consequent steps of oxidation and PEG-g-PEI polyionic complexation (PEG-PEI-SWNT). The DOX loading capacity of modified SWNTs was about 900%. Moreover, it showed an enhanced dispersibility in physiologic-stimulated medium. DOX release was prolonged, independent of dilution, and exhibited an acidic pH-stimulated release. Therefore, PEG-PEI-SWNT could be used for cancer chemotherapy in vivo.

  19. Riparian Habitat - San Joaquin River

    Data.gov (United States)

    California Department of Resources — The immediate focus of this study is to identify, describe and map the extent and diversity of riparian habitats found along the main stem of the San Joaquin River,...

  20. 1906 San Francisco, USA Images

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The 1906 San Francisco earthquake was the largest event (magnitude 8.3) to occur in the conterminous United States in the 20th Century. Recent estimates indicate...

  1. Histopathological finding of liver and kidney tissues of the yellow mystus, Hemibagrus filamentus (Fang and Chaux, 1949, from the Tapee River, Thailand

    Directory of Open Access Journals (Sweden)

    Sinlapachai Senarat

    2015-02-01

    Full Text Available A histological finding of liver and kidney tissues of yellow mystus, Hemibagrus filamentus (Fang and Chaux, 1949, collected from the Tapee River (Chawang District, Nakhon Si Thammarat Province, Thailand was conducted to examine the pathological lesions. Lesions, including cellular swelling, hydropic degeneration, or vacuolation of hepatocytes were found. From this, coagulative necrosis with pyknotic nuclei, infiltration of macrophage, constriction of sinusoidal capillaries and damage of endothelial cells of blood vessels were observed in liver tissues. The dilation of both Bowman’s space and the glomerulus were observed from kidney lesions. Moreover, necrosis in renal tubular cells was also observed in some areas. Although preliminary, the results from this study clearly show the need for further investigation into stress factors and vectors affecting the health of H. filamentus and other fish species within the Tapee River.

  2. 细说方鸿渐生活中的五位女性%Detailed Analyse of Five Women in Fang Hongjian's Life

    Institute of Scientific and Technical Information of China (English)

    张芙蓉

    2011-01-01

    Fang Hongjian was a main figure in the works "Weicheng".The five women who lived at his side was the represents of socia stratum people during the semi-colonial and semi-feudal society.Theirs speech and deportment reflected the social appeerence,also disclosed every aspect of people influenced by western culture and by tradition at that time.%方鸿渐是《围城》贯穿始终的主要人物。活跃在他身边的五位女性,是当时社会多层面人物的代表。她们的言行举止反映了当时的社会风貌。揭示出当时半殖民地半封建社会下,深受西方文化熏陶的国人及传统国民灵魂的方方面面。

  3. O ENVELHECIMENTO, A DOENÇA DE ALZHEIMER E AS CONTRIBUIÇÕES DO PROGRAMA DE ENRIQUECIMENTO INSTRUMENTAL (PEI

    Directory of Open Access Journals (Sweden)

    Antonia Rozeli Roberto de Oliveira

    2010-01-01

    Full Text Available Ese artículo tiene como propuesta presentar el envejecimiento como un proceso individualizado con potencialidades únicas y distintas, haciendo distinción entre la vejez normal y la patológica. Se enfatiza que el envejecimiento exitoso depende en gran parte de la prevención de enfermedades, adecuado condicionamiento físico y preservación de las funciones cognitivas. Sin embargo, estudios epidemiológicos señalan que ancianos con ligeras alteraciones cognitivas presentan un mayor riesgo de desarrollar la Enfermedad de Alzheimer (EA, considerada el diagnóstico más frecuente entre las demencias. Considerándose que esa condición afecta el cerebro, ocasionando perdida de la memoria y el comprometimiento en varias áreas cognitivas (lenguaje, función ejecutiva, praxia, gnosi, etc. son propuestas alternativas de tratamiento más allá de la terapéutica farmacológica caracterizando la rehabilitación neuropsicológica. En ese estudio, en especial, intentase abordar las posibilidades de uso del Programa de Enriquecimiento Instrumental (PEI y de la Experiencia de la Aprendizaje Mediada (EAM en los programas de rehabilitación cognitiva direccionado a la EA, visando favorecer la optimización de las capacidades residuales y disminuir el impacto negativo en la cualidad de vida de estas personas.

  4. Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals

    Directory of Open Access Journals (Sweden)

    Jinhui Cui

    2012-01-01

    Full Text Available To evaluate the performance of the magnetic nanoparticles as gene transfer vector for breeding transgenic animals, we investigated a new approach to deliver green fluorescent protein (GFP gene to porcine kidney 15 (PK-15 and porcine embryonic fibroblast (PEF cells using PEI-modified magnetic nanoparticles as gene vector. The morphology of the nanoparticles and nanoparticle/DNA complexes was characterized using scanning electron microscopy. It was found that the surface of the particles becomes coarse and rough with increased average diameter, which implied the effective conjugating between nanoparticles with DNA. The zeta potential of nanoparticle/DNA complexes drops down from +29.4 mV to +23.1 mV comparing with pure nanoparticles. Agarose gel electrophoresis experiments show that DNA plasmids can be protected effectively against degradation of exonuclease and endonuclease. The efficiency of gene delivery was affected by the mass ratio of nanoparticle/DNA and the amount of nanoparticle/DNA complexes. We confirm that the most optimal mass ratio of nanoparticle/DNA is 1  :  1 by conducting a series of experiments. This work provides important experimental basis for the application of the magnetic nanoparticles on gene delivery to porcine somatic cells, which is significant for the achieving of breeding new transgenic cloned pigs by using somatic cell nuclear transfer technique.

  5. Water absorption in PEEK and PEI matrices. Contribution to the understanding of water-polar group interactions

    Science.gov (United States)

    Courvoisier, E.; Bicaba, Y.; Colin, X.

    2016-05-01

    The water absorption in two aromatic linear polymers (PEEK and PEI) was studied between 10% and 90% RH at 30, 50 and 70°C. It was found that these polymers display classical Henry and Fick's behaviors. Moreover, they have very close values of equilibrium water concentration C∞ and water diffusivity D presumably because their respective polar groups establish molecular interactions of the same nature with water. This assumption was checked from a literature compilation of values of C∞ and D for a large variety of linear and tridimensional polymers containing a single type of polar group. It was then evidenced that almost all types of carbonyl group (in particular, those belonging to imides, amides and ketones) have the same molar contribution to water absorption, except those belonging to esters which are much less hydrophilic. Furthermore, hydroxyl and sulfone groups are much more hydrophilic than carbonyl groups so that their molar contribution is located on another master curve. On this basis, semi-empirical structure/water transport property relationships were proposed. It was found that C∞ increases exponentially with the concentration of polar groups (presumably because water is doubly bonded), but also with the intensity of their molecular interactions with water. In contrast, D is inversely proportional to C∞, which means that polar group-water interactions slow down the rate of water diffusion.

  6. Environmental assessment : Rodent control program : San Joaquin river levee : San Luis National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Lower San Joaquin Levee District (LSJLD) requires that six miles of levee situated along the San Joaquin River on San Luis National Wildlife Refuge (SLNWR) be...

  7. Probing the Role of Zr Addition versus Textural Properties in Enhancement of CO 2 Adsorption Performance in Silica/PEI Composite Sorbents

    Energy Technology Data Exchange (ETDEWEB)

    Sakwa-Novak, Miles A.; Holewinski, Adam; Hoyt, Caroline B.; Yoo, Chun-Jae; Chai, Song-Hai; Dai, Sheng; Jones, Christopher W.

    2015-09-01

    Polymeric amines such as poly(ethylenimine) (PEI) supported on mesoporous oxides are promising candidate adsorbents for CO2 capture processes. An important aspect to the design and optimization of these materials is a fundamental understanding of how the properties of the oxide support such as pore structure, particle morphology, and surface properties affect the efficiency of the guest polymer in its interactions with CO2. Previously, the efficiency of impregnated PEI to adsorb CO2 was shown to increase upon the addition of Zr as a surface modifier in SBA-15. However, the efficacy of this method to tune the adsorption performance has not been explored in materials of differing textural and morphological nature. Here, these issues are directly addressed via the preparation of an array of SBA-15 support materials with varying textural and morphological properties, as well as varying content of zirconium doped into the material. Zirconium is incorporated into the SBA-15 either during the synthesis of the SBA-15, or postsynthetically via deposition of Zr species onto pure-silica SBA-15. The method of Zr incorporation alters the textural and morphological properties of the parent SBA-15 in different ways. Importantly, the CO2 capacity of SBA-15 impregnated with PEI increases by a maximum of ~60% with the quantity of doped Zr for a “standard” SBA-15 containing significant microporosity, while no increase in the CO2 capacity is observed upon Zr incorporation for an SBA-15 with reduced microporosity and a larger pore size, pore volume, and particle size. Finally, adsorbents supported on SBA-15 with controlled particle morphology show only modest increases in CO2 capacity upon inclusion of Zr to the silica framework. The data demonstrate that the textural and morphological properties of the support have a more significant impact on the ability of PEI to capture CO2 than the support surface composition.

  8. Residencia San Pedro, California

    Directory of Open Access Journals (Sweden)

    Neutra, Richard J.

    1961-01-01

    Full Text Available Esta vivienda representa una aproximación más hacia la típica casa grande española, con techos de teca de 7 cm, que los señores Rados han edificado y en la que albergan a su gran familia de hijos, los cuales tienen ya sus propios vástagos. Ambos, el señor y la señora Rados, descienden de familias navieras italianas de Trieste, y el propio señor Rados tiene una compañía constructora de barcos en el puerto de San Pedro, que puede verse desde su propia casa. Los dos son verdaderamente unos abuelos muy sociables, cariñosos y atentos. Por añadidura, la señora Rados se entretiene frecuentemente y le agrada el cuidado de la casa. Por ello ha sido proyectada para facilitar sensiblemente toda esta serie de actividades.

  9. Electrochemical non-enzyme sensor for detecting clenbuterol (CLB) based on MoS2-Au-PEI-hemin layered nanocomposites.

    Science.gov (United States)

    Yang, Yayun; Zhang, Huan; Huang, Chusen; Yang, Dapeng; Jia, Nengqing

    2017-03-15

    Clenbuterol (CLB), a kind of β2-adrenergic agonists, could disrupt cardiovascular and central nervous system. In this work, a new electrochemical non-enzyme sensor for detecting Clenbuterol (CLB) was fabricated based on MoS2-Au-PEI-hemin layered nanocomposites. The two-dimensional (2-D) MoS2 nanosheets were first in-situ assembled with Au nanoparticles, and polyethylenimine (PEI), then hemin molecules were immobilized onto the MoS2-Au-PEI film-modified glassy carbon electrode (GCE) via amide bond. Scanning electron microscopy (SEM) and Zeta potential measurements were employed to characterize the MoS2-based nanomaterials. Cyclic voltammetry (CV) was used to investigate electrochemical activity of the immobilized hemin on the modified electrode. Upon the optimum conditions, the proposed electrochemical sensor showed an excellent response for CLB including a wide linear ranging from 10ng/mL to 2μg/mL and a detection limit (LOD) of 1.92ng/mL CLB (S/N=3) with favorable reproducibility and stability. Furthermore, this presented method could be feasible for determining CLB in the real pork samples.

  10. Dexamethasone-loaded reconstitutable charged polymeric (PLGA)n -b-bPEI micelles for enhanced nuclear delivery of gene therapeutics.

    Science.gov (United States)

    Mishra, Deepa; Kang, Han Chang; Cho, Hana; Bae, You Han

    2014-06-01

    This study investigates the potential of dexamethasone (Dex) to enhance the nuclear accumulation and subsequent gene expression of plasmid DNA (pDNA) delivered using a charged polymeric micelle-based gene delivery system. (PLGA)n -b-bPEI25kDa block copolymers are synthesized and used to prepare Dex-loaded cationic micelles (DexCM). After preparing DexCM/pDNA complexes, bPEI1.8kDa is coated on the complexes using a Layer-by-Layer (LbL) technique to construct DexCM/pDNA/bPEI1.8kDa complexes (i.e., LbL-DexCM polyplexes) that are 100-180 nm in diameter and have a zeta potential of 30-40 mV. In MCF7 cells, LbL-DexCM polyplexes cause 3-13-fold higher transfection efficiencies compared to LbL-CM polyplexes and show negligible cytotoxicity. LbL-DexCM3 polyplexes induce much higher nuclear delivery of pDNA compared to LbL-CM3 polyplexes. These results suggest that Dex-loaded polyplexes could be used in gene and drug delivery applications to increase nuclear accumulation of therapeutic payloads, further leading to a decrease in the dose of the drug and gene necessary to achieve equivalent therapeutic effects.

  11. Effect of Hydrogen Reduction of Silver Ions on the Performance and Structure of New Solid Polymer Electrolyte PEI/Pebax2533/AgBF4 Composite Membranes

    Institute of Scientific and Technical Information of China (English)

    WANG Yanbei; REN Jizhong; LI Hui; DENG Maicun

    2013-01-01

    In this paper,the effect of hydrogen reduction of silver ions on the performance and structure of new solid polymer electrolyte polyetherimide (PEI)/Pebax2533 (Polynylon12/tetramethylene oxide block copolymer,PA12-PTMO)/AgBF4 composite membranes is investigated.For PEI/Pebax2533/AgBF4 composite membranes prepared with different AgBF4 concentration,the permeances of propylene and ethylene increase with the increase of AgBF4 concentration due to the carrier-facilitated transport,resulting in a high selectivity.But for propylene/propane mixture,the mixed-gas selectivity is lower than its ideal selectivity.The hydrogen reduction strongly influences the membrane performance,which causes the decrease of propylene permeance and the increase of propane permeance.With the increase of hydrogen reduction time,the membranes show a clearly color change from white to brow0n,yielding a great selectivity loss.The data of X-ray diffraction and FT-IR prove that silver ions are reduced to Ag after hydrogen reduction,and aggregated on the surface of PEI/Pebax2533/AgBF4 composite membranes.

  12. mPEG-PCL-g-PEI聚合物纳米粒介导的小干扰RNA递送%Small interfering RNA delivery mediated by mPEG-PCL-g-PEI polymer nanoparticles

    Institute of Scientific and Technical Information of China (English)

    黄伟; 吕明; 高钟镐; 金明姬; 杨长青

    2011-01-01

    本文旨在合成小干扰核糖核酸(siRNA)递送载体聚合物聚乙二醇-聚己内酯-聚乙烯亚胺(mPEG-PCL-g-PEI),并探讨其体外siRNA递送性能.通过开环聚合反应制备二嵌段共聚物聚乙二醉-聚己内酯(mPEG-PCL-OH),将mPEG-PCL-OH的羟基末端依次化学转化为羟基(-COOH)和N-羟基琥珀酰亚胺(-NHS)生成mPEG-PCL-NHS,再将mPEG-PCL-NHS同枝化聚乙烯亚胺(PEI)反应生成三元共聚物mPEG-PCL-g-PEI.应用傅里叶变换红外光谱(FTIR)、核磁共振(NMR)和凝胶渗透色谱(GPC)对聚合物mPEG-PCL-g-PEI进行结构表征;通过复凝聚法制备mPEG-PCL-g-PEI/siRNA纳米粒,并测定其粒径和zeta电位;通过体外细胞MTT测试,比较mPEG-PCL-g-PEI/siRNA纳米粒和PEI/siRNA纳米粒的细胞毒性;通过体外细胞转染实验,考察不同N/P比的mPEG-PCL-g-PEI/siRNA纳米粒对萤火虫荧光素酶基因表达的抑制效率.结果表明,合成的三元聚合物(MPEG5k-PCL1.2k)1.4-g-PEI10k能压缩siRNA形成粒径为50~200 mn的纳米粒,其表面带有正电荷.MTT分析结果显示(mPEG5k-PCL1.2k)1.4-g-PEI10k/siRNA纳米粒的细胞毒性显著小于PEI10K/siRNA纳米粒(P< 0.05).当N/P比在50~150,萤火虫荧光素酶基因的表达显著下调(P<0.01).当N/P比为125时,萤火虫荧光素酶基因表达的抑制效率最大.聚合物mPEG-PCL-g-PEI能递送siRNA进入细胞,抑制靶基因表达,且细胞毒性较低,有望成为一种新型的siRNA递送载体.%The aim of this paper is to report the synthesis of the mPEG-PCL-g-PEI copolymers as small interfering RNA (siRNA) delivery vector, and exploration of the siRNA delivery potential of mPEG-PCL-g-PEI in vitro. The diblock copolymers mPEG-PCL-OH was prepared through the ring-opening polymerization. Then, the hydroxyl terminal (-OH) of mPEG-PCL-OH was chemically converted into the carboxy (-COOH) and N-hydroxysuccinimide (NHS) in turn to prepare mPEG-PCL-NHS. The branched PEI was reacted with mPEG-PCL-NHS to synthesize the ternary

  13. 沛县蔬菜生态系统能值分析%Emergy analysis of vegetable ecosystem in Pei County, Jiangsu Province

    Institute of Scientific and Technical Information of China (English)

    付伟; 蒋芳玲; 刘洪文; 吴震

    2011-01-01

    县蔬菜产业发展迅速;但净能值产出率仅略高于江苏省耕地生态系统净能值产出率,可能由于能值产出较高的先进技术推广不够;沛县蔬菜生态系统环境负荷率为1.43,低于江苏省耕地生态系统环境负荷率(2.83),且农药能值投入仅占0.21%,说明沛县蔬菜产业有较强的可持续发展能力和较大的发展潜力.为促进沛县蔬菜产业综合效益的提高和可持续发展,应进一步加强蔬菜产业基础设施及蔬菜产品加工业的建设,加大蔬菜产业的科学技术推广力度,增强沛县蔬菜生态经济系统的产出能力.%The theory of emergy analysis is based on the premise that all forms of energy are from the sun. Different and incomparable forms of energy are therefore unifiable into solar emjoules. In this study, environmental resources and economic characteristics of vegetable ecosystems in Pei County (one of the main production bases of vegetable in Jiangsu Province) were quantitatively analyzed using the theories and methods of ecosystem emergy analysis. A series of emergy indices were developed for evaluating the environment resources and development characters of the vegetable ecosystem in Pei County. These indices simultaneously provided scientific basis for sustainable development of vegetable industry in Pei County. The study showed that: in 2007, vegetable emergy input in Pei County was 1.22Χ 1021 sej. Industrial auxiliary energy input was 7.18Χ 1020 sej, accounting for 58.85% of the total vegetable ecosystem emergy, which suggested that the development of vegetable industry in Pei County relied mainly on industrial auxiliary energy, and had developed beyond traditional agriculture relied mainly on natural conditions. Vegetable ecosystem output in PeiCounty was 3.20Χ 1021 sej, accounting for 39.65% of total agriculture emergy yield, indicating that vegetable industry was one of the strongest pillars of industries in Pei County. Emergy outputs from

  14. 《外台秘要方》与《太平圣惠方》中伤寒理论文献的比较%Comparative study of theoretical literature on cold pathogenic disease in Wai tai mi yao fang (Arcane Essentials from the Imperial Library) and Tai ping sheng hui fang (Taiping Holy Prescriptions for Universal Relief)

    Institute of Scientific and Technical Information of China (English)

    张慧蕊; 梁永宣

    2014-01-01

    In the Wai tai mi yao fang (Arcane Essentials from the Imperial Library) compiled in 752,its portion on cold pathogenic disorders embodies the achievements before the mid Tang Dynasty,whereas that in the Tai ping sheng hui fang (Taipng Holy Prescriptions for Universal Relief),compiled in 992 embodies those before the early Song Dynasty.Comparison on the theory of cold disorders in both books reveal that,during the 2 centuries period from mid Tang to early Song Dynasties,the texts as a carrier for the transmission of such theory in both show no distinct changes,but only with minor revisions and improvements.%唐天宝十年(752)成书的《外台秘要方》中的伤寒部分,汇总了中唐及其以前的伤寒成果,而宋淳化三年(992)成书的《太平圣惠方》中的伤寒内容,则汇集了宋初及以前诸家伤寒之大成.对2书中伤寒理论部分进行比较,可以发现从中唐至宋初200年间,以“文本”为载体的伤寒理论基本没有大的变化,仅有少量的修正和完善.

  15. 正电型PVC/PEI复合纳滤膜的制备及其盐/染料分离性能∗%Fabrication anddye-salt separation of positively charged PVC/PEI composite nanofiltration membranes

    Institute of Scientific and Technical Information of China (English)

    崔月; 许云秋; 姚之侃; 杜世媛; 赵斌; 朱宝库; 朱利平

    2014-01-01

    在聚氯乙烯(PVC)超滤膜表面通过沉积形成与之共价结合的聚乙烯亚胺(PEI)层,先后用戊二醛和2,3-环氧丙基三甲基氯化铵(EPTAC)令 PEI 交联和季铵化,制备了荷正电 PVC/PEI 复合纳滤膜;考察PEI分子量和 EPTAC 浓度对膜结构及性能的影响.结果表明,当分子量分别为5万和7万的两种 PEI 按质量比1∶1混合使用时,可以得到皮层致密均一的复合膜,EPTAC 季铵化后 PVC/PEI 复合纳滤膜荷正电,有良好的亲水性,0.3 MP a 操作压力下水通量达58.7 L/m2·h,对 NaCl 不截留,对刚果红染料的截留率达98.9%,在染料和无机盐分离领域具有重要意义.%By filtering deposition of polyethylene imine (PEI)and then crosslinking by glutaraldehyde,a dense separation layer was introduced onto the surface of PVC ultrafiltration membrane.On this basis,positively charged PVC/PEI composite NF membrane was prepared after the reaction between the remaining amino groups and 2,3-epoxy-trimethyl ammonium chloride(EPTAC).It was found that we would achieve PVC nanofiltration membrane with the best performance using mixture of PEI-7w and PEI-2w (1∶1 ).The PVC/PEI composite NF membrane showed good hydrophilicity and great nanofiltration performance.Under the operating pressure of 0.3 MPa,the Congo red rejection and flux is 98.9% and 58.7 L/m2 ·h,respectively,while the NaCl rejection is almost zero.Thus,the PVC nanofiltration membrane was quite qualified for the separation of mixed inorgan-icsalt and dye solution.This approach is a promising way for fabricating PVC NF membrane with high water flux and dye rej ection.

  16. Vegetation - San Felipe Valley [ds172

    Data.gov (United States)

    California Department of Resources — This Vegetation Map of the San Felipe Valley Wildlife Area in San Diego County, California is based on vegetation samples collected in the field in 2002 and 2005 and...

  17. 75 FR 39166 - Safety Zone; San Francisco Giants Baseball Game Promotion, San Francisco, CA

    Science.gov (United States)

    2010-07-08

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Francisco Giants Baseball Game... Bay off San Francisco, CA in support of the San Francisco Giants Baseball Game Promotion. This safety... Giants will sponsor the San Francisco Giants Baseball Game Promotion on July 16, 2010, on the...

  18. 78 FR 21403 - Notice of Inventory Completion: San Francisco State University NAGPRA Program, San Francisco, CA

    Science.gov (United States)

    2013-04-10

    ... NAGPRA Program, c/o Department of Anthropology, San Francisco State University, 1600 Holloway Avenue, San...: Pursuant to 25 U.S.C. 3001(9), the human remains described in this notice represent the physical remains of... NAGPRA Program, c/o Department of Anthropology, San Francisco State University, 1600 Holloway Avenue, San...

  19. 76 FR 55796 - Safety Zone; TriRock Triathlon, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2011-09-09

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; TriRock Triathlon, San Diego Bay, San Diego.... Basis and Purpose Competitor Group is sponsoring the TriRock Triathlon, consisting of 2000 swimmers....T11-431 Safety Zone; TriRock Triathlon, San Diego Bay, San Diego, CA. (a) Location. The limits of...

  20. 76 FR 45693 - Safety Zone; San Diego POPS Fireworks, San Diego, CA

    Science.gov (United States)

    2011-08-01

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego POPS Fireworks, San Diego, CA... temporary safety zone on the navigable waters of San Diego Bay in support of the San Diego POPS Fireworks... Diego, CA; telephone (619) 278- 7262, e-mail Shane.E.Jakcson@uscg.mil . If you have questions on viewing...

  1. 78 FR 38584 - Safety Zone; San Diego Symphony Summer POPS Fireworks 2013 Season, San Diego, CA

    Science.gov (United States)

    2013-06-27

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego Symphony Summer POPS Fireworks 2013 Season, San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone on the navigable waters of San Diego Bay in support of the San Diego...

  2. 76 FR 75908 - Notice of Inventory Completion: The University of California, San Diego, San Diego, CA

    Science.gov (United States)

    2011-12-05

    ... National Park Service Notice of Inventory Completion: The University of California, San Diego, San Diego... California on behalf of the University of California, San Diego, have completed an inventory of human remains... contact the University of California, San Diego. Disposition of the human remains and associated funerary...

  3. 77 FR 42647 - Safety Zone: San Diego Symphony POPS Fireworks; San Diego, CA

    Science.gov (United States)

    2012-07-20

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone: San Diego Symphony POPS Fireworks; San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone on the navigable waters of San Diego Bay in support of the San Diego Symphony POPS...

  4. 75 FR 77756 - Safety Zone; San Diego Parade of Lights Fireworks, San Diego, CA

    Science.gov (United States)

    2010-12-14

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; San Diego Parade of Lights Fireworks, San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone upon the navigable water of the San Diego Bay in San Diego, CA in support of the two...

  5. The San Bernabe power substation; La subestacion San Bernabe

    Energy Technology Data Exchange (ETDEWEB)

    Chavez Sanudo, Andres D. [Luz y Fuerza del Centro, Mexico, D. F. (Mexico)

    1997-12-31

    The first planning studies that gave rise to the San Bernabe substation go back to year 1985. The main circumstance that supports this decision is the gradual restriction for electric power generation that has been suffering the Miguel Aleman Hydro System, until its complete disappearance, to give priority to the potable water supply through the Cutzamala pumping system, that feeds in an important way Mexico City and the State of Mexico. In this document the author describes the construction project of the San Bernabe Substation; mention is made of the technological experiences obtained during the construction and its geographical location is shown, as well as the one line diagram of the same [Espanol] Los primeros estudios de planeacion que dieron origen a la subestacion San Bernabe se remontan al ano de 1985. La circunstancia principal que soporta esta decision es la restriccion paulatina para generar energia que ha venido experimentando el Sistema Hidroelectrico Miguel Aleman, hasta su desaparicion total, para dar prioridad al suministro de agua potable por medio del sistema de bombeo Cutzamala, que alimenta en forma importante a la Ciudad de Mexico y al Estado de Mexico. En este documento el autor describe el proyecto de construccion de la subestacion San Bernabe; se mencionan las experiencias tecnologicas obtenidas durante su construccion y se ilustra su ubicacion geografica, asi como un diagrama unifilar de la misma

  6. Shen-Qi-Jie-Yu-Fang exerts effects on a rat model of postpartum depression by regulating inflammatory cytokines and CD4+CD25+ regulatory T cells

    Directory of Open Access Journals (Sweden)

    Li JY

    2016-04-01

    Full Text Available Jingya Li,1,* Ruizhen Zhao,1,* Xiaoli Li,1 Wenjun Sun,1 Miao Qu,1 Qisheng Tang,1 Xinke Yang,1 Shujing Zhang2 1Third Affiliated Hospital, 2School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, People’s Republic of China *These authors contributed equally to this work Background: Shen-Qi-Jie-Yu-Fang (SJF is composed of eight Chinese medicinal herbs. It is widely used in traditional Chinese medicine for treating postpartum depression (PPD. Previous studies have shown that SJF treats PPD through the neuroendocrine mechanism. Aim: To further investigate the effect of SJF on the immune system, including the inflammatory response system and CD4+CD25+ regulatory T (Treg cells. Materials and methods: Sprague Dawley rats were used to create an animal model of PPD by inducing hormone-simulated pregnancy followed by hormone withdrawal. After hormone withdrawal, the PPD rats were treated with SJF or fluoxetine for 1, 2, and 4 weeks. Levels of Treg cells in peripheral blood were measured by flow cytometry analysis. Serum interleukin (IL-1β and IL-6 were evaluated by enzyme-linked immunosorbent assay, and gene and protein expressions of IL-1RI, IL-6Rα, and gp130 in the hippocampus were observed by reverse-transcription polymerase chain reaction and Western blot. Results: Serum IL-1β in PPD rats increased at 2 weeks and declined from then on, while serum IL-6 increased at 1, 2, and 4 weeks. Both IL-1β and IL-6 were downregulated by SJF and fluoxetine. Changes in gene and protein expressions of IL-1RI and gp130 in PPD rats were consistent with changes in serum IL-1β, and were able to be regulated by SJF and fluoxetine. The levels of Treg cells were negatively correlated with serum IL-1β and IL-6, and were decreased in PPD rats. The levels of Treg cells were increased by SJF and fluoxetine. Conclusion: Dysfunction of proinflammatory cytokines and Tregs in different stages of PPD was attenuated by SJF and fluoxetine through

  7. A Spanish Borderlands Community: San Antonio.

    Science.gov (United States)

    Teja, Jesus F. de la

    2000-01-01

    Discusses the founding of San Antonio, originally San Antonio de Bexar, which, in 1718, came into being as a military settlement involved in Spanish imperial defensive measures. Focuses on the development and continued growth of San Antonio, Texas's most populous city in the 19th century. (CMK)

  8. Convair Astronautics, San Diego (California

    Directory of Open Access Journals (Sweden)

    Pereira & Luckmam, Arquitectos

    1960-05-01

    Full Text Available Este brillante y espectacular complejo industrial se ha creado especialmente para la investigación y fabricación de cohetes intercontinentales y vehículos del espacio de las Fuerzas Aéreas de los EE. UU., en las proximidades de San Diego y cerca del campo de pruebas de Sycamore Canyon.

  9. Enhanced delivery of Paclitaxel using electrostatically-conjugated Herceptin-bearing PEI/PLGA nanoparticles against HER-positive breast cancer cells.

    Science.gov (United States)

    Yu, Kongtong; Zhao, Jinlong; Zhang, Zunkai; Gao, Yin; Zhou, Yulin; Teng, Lesheng; Li, Youxin

    2016-01-30

    We have developed a novel nanoparticle delivery system fabricated from polyethylenimine (PEI) and poly(d,l-lactide-co-glycolide) (PLGA), which were able to deliver the chemotherapeutic agent Paclitaxel, while the biomacromolecule Herceptin acted as a targeting ligand that was conjugated onto the surfaces of the nanoparticles via electrostatic interactions. In this study, these electrostatically-conjugated Herceptin-bearing PEI/PLGA nanoparticles (eHER-PPNs) were optimized and employed as vectors to target HER2-positive breast cancer cells. The eHER-PPNs had an average diameter of ∼ 280 nm and a neutral surface charge (1.00 ± 0.73 mV), which remained stable under physiological conditions. The anticancer effects of eHER-PPNs were investigated in HER2-positive BT474 cells and HER2-negative MCF7 cells. The eHER-PPNs showed enhanced cytotoxicity that was dependent on the receptor expression levels and the incubation time. These conjugated nanoparticles deliver Paclitaxel more efficiently (pHerceptin and the combined effects of these two monotherapies. Furthermore, the chemically-conjugated Herceptin-bearing PEI/PLGA nanoparticles (cHER-PPNs) were fabricated as a comparison. The eHER-PPNs exhibited lower cell viability (46.7%) than that of cHER-PPNs (65.1%). The targeting ability of eHER-PPNs was demonstrated through confocal microscopy images and flow cytometry, which showed that eHER-PPNs displayed higher cellular uptake efficiency (p<0.001) in comparison with cHER-PPNs. Therefore, eHER-PPNs could provide promising platforms for the delivery of therapeutic drugs against HER2-positive breast cancers.

  10. Synthesis and characterization of Fe3O4-PEG-LAC-chitosan-PEI nanoparticle as a survivin siRNA delivery system.

    Science.gov (United States)

    Arami, S; Rashidi, M R; Mahdavi, M; Fathi, M; Entezami, A A

    2017-03-01

    The limited effectiveness of the conventional methods for cancer treatment makes the researchers to find novel safe and effective therapeutic strategies. One of these strategies is to use small interfering RNAs (siRNAs). A major challenge here is the siRNA delivery into the cells. The purpose of this study was to design and prepare a biocompatible, biodegradable, and safe nanosized particle for siRNA delivery into human breast cancer MCF-7 and leukemia K562 cells. Chemically synthesized magnetic nanoparticles containing polyethyleneglycol-lactate polymer (PEG-LAC), chitosan, and polyethyleneimine (PEI) were successfully prepared and used as a gene delivery vehicle. The nanoparticles were characterized by Fourier transform infrared spectroscopy and zeta potential. The Fe3O4-PEG-LAC-chitosan-PEI nanoparticle showed efficient and stable survivin siRNA loading in gel retardation assay. The cytotoxicity of the prepared nanoparticle was studied using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay and was compared with that of mitoxantrone (MTX) in combination with the prepared siRNA delivery system to evaluate the possible synergic effect of MTX and survivin siRNA. The nanoparticles with and without noncomplementary siRNA showed low toxicity against both cell lines; however, a twofold decrease was observed in cell survival percent after MTX addition to MCF-7 cells treated with either nanoparticle itself or complexed with noncomplementary siRNA. While survivin siRNA nanoplex caused threefold decrease in the cell survival percent, its combination with MTX did not result in a significant increase in the cytotoxic effect. Therefore, Fe3O4-PEG-LAC-chitosan-PEI nanoparticle should be considered as a potential carrier for enhanced survivin siRNA delivery into MCF-7 and K562 cells.

  11. On-line monitoring the extract process of Fu-fang Shuanghua oral solution using near infrared spectroscopy and different PLS algorithms

    Science.gov (United States)

    Kang, Qian; Ru, Qingguo; Liu, Yan; Xu, Lingyan; Liu, Jia; Wang, Yifei; Zhang, Yewen; Li, Hui; Zhang, Qing; Wu, Qing

    2016-01-01

    An on-line near infrared (NIR) spectroscopy monitoring method with an appropriate multivariate calibration method was developed for the extraction process of Fu-fang Shuanghua oral solution (FSOS). On-line NIR spectra were collected through two fiber optic probes, which were designed to transmit NIR radiation by a 2 mm flange. Partial least squares (PLS), interval PLS (iPLS) and synergy interval PLS (siPLS) algorithms were used comparatively for building the calibration regression models. During the extraction process, the feasibility of NIR spectroscopy was employed to determine the concentrations of chlorogenic acid (CA) content, total phenolic acids contents (TPC), total flavonoids contents (TFC) and soluble solid contents (SSC). High performance liquid chromatography (HPLC), ultraviolet spectrophotometric method (UV) and loss on drying methods were employed as reference methods. Experiment results showed that the performance of siPLS model is the best compared with PLS and iPLS. The calibration models for AC, TPC, TFC and SSC had high values of determination coefficients of (R2) (0.9948, 0.9992, 0.9950 and 0.9832) and low root mean square error of cross validation (RMSECV) (0.0113, 0.0341, 0.1787 and 1.2158), which indicate a good correlation between reference values and NIR predicted values. The overall results show that the on line detection method could be feasible in real application and would be of great value for monitoring the mixed decoction process of FSOS and other Chinese patent medicines.

  12. Renoprotective Effect of the Shen-Yan-Fang-Shuai Formula by Inhibiting TNF-α/NF-κB Signaling Pathway in Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Jie Lv

    2017-01-01

    Full Text Available Diabetic kidney disease (DKD is the leading cause of end-stage kidney disease, and satisfactory therapeutic strategies have not yet been established. The Shen-Yan-Fang-Shuai Formula (SYFSF is a traditional Chinese formula composed of Astragali radix, Radixangelicae sinensis, Rheum officinale Baill, and four other herbs. It has been widely used as an effective treatment for DKD patients in China. However, little is known about the molecular mechanisms underlying SYFSF’s renoprotection. In this study, we compared the protective effect of SYFSF to irbesartan on the histology and renal cells in type 2 DKD rat model and high-glucose (HG cultured mesangial cells, respectively. We found that SYFSF could significantly decrease urinary albumin, cholesterol, and triglyceride. And a decrease in serum creatinine was also found in SYFSF-treated group compared with irbesartan-treated rats. In addition, SYFSF inhibited the interstitial expansion and glomerulosclerosis in diabetic rats. Notably, SYFSF markedly downregulated the expression of MCP-1, TGF-β1, collagen IV, and fibronectin in diabetic rat models and HG-induced mesangial cell models. The renoprotection was closely associated with a reduced expression of TNF-α and phosphorylated NF-κBp65. Our study suggests that SYFSF may ameliorate diabetic kidney injury. The observed renoprotection is probably attributable to an inhibition of inflammatory response and extracellular matrix (ECM accumulation mediated by TNF-α/NF-κBp65 signaling pathway.

  13. 浅谈方有执对《伤寒论》的发挥%Introduction the Viewpoint of FANG Youzhi to SHANGHANLUN

    Institute of Scientific and Technical Information of China (English)

    谢韬

    2015-01-01

    新安医家方有执通过错简重订《伤寒论》,认为伤寒应以六经为纲,六经以太阳为纲,太阳以风伤卫、寒伤营、风寒两伤营卫为纲。揭示了伤寒的发病、转归规律,为《伤寒论》的研究提供了新的方向。%Xin'an physician Fang Youzhi by booking ShangHan Scince , thinking of typhoid fever should be with the six classics as the key link, the six classics to the sun as the key link, the sun to the wind hurt Wei, cold injury camp, cold injury to both Ying Wei gang.Revealing the pathogenesis of typhoid, transferring patterns.Provides a new research direction for the study of the ShangHan Scince.

  14. Professor WANG Xia-fang's Experience on Treating Asthma in Children%王霞芳治疗小儿哮喘经验

    Institute of Scientific and Technical Information of China (English)

    吴岚莹; 封玉林

    2012-01-01

    This article introduced professor WANG Xia-fang' s experience on treating asthma in children. It is held that acute attack of asthma in children results from invasion of exogenous wind and accumulation of endogenous phlegm-fluid; its treatment focused on releasing lung and resolving phlegm,relieving coughing and suppressing asthma,and dispelling wind and dregding collaterals. Xuanfei Tongluo Pingchuan Decoction was used in clinical practice. In paracmasis we applied the method of strengthening earth to generate metal to invigorate the spleen and to help transportation and transformation,cutting off the. source of eliminating phlegm and reducing asthma palindromia. The curative is obviously effective.%介绍王霞芳教授分期治疗小儿哮喘的经验.认为小儿哮喘急性发作期以风邪外袭,痰饮内伏,致肺失宣降为主要病因病机,治疗以宣肺化痰、止咳平喘、祛风通络,选用自拟“宣肺通络平喘汤”;缓解期用“培土生金法”健脾助运,杜绝生痰之源,治痰饮之本减少哮喘复发,疗效显著.

  15. Fang Zhongyan’s Legal Thought and Reform%范仲淹的法律思想与法制变革

    Institute of Scientific and Technical Information of China (English)

    郭东旭; 郭瑞童

    2013-01-01

    作为一位以天下为己任的政治家,范仲淹具有修德省刑的法律思想和精审法令的立法主张。在法制变革的实践中,范仲淹又持有令出为行的执法理念、明法慎罚的用法原则。其法律思想集中体现了儒家重情恤刑的慎法精神。%Fang Zhongyan as a politician who regards the whole society as his own duty has the legal thought of strengthening morality and reducing penalty. He also advocates that law enforcing officials should obey the law and adhere to the principles of the law strictly in the practice of legal reform. His legal thought reflects the spirit of Confucian which stands for the value of feelings and using penalty cautiously.

  16. Effect of TongXie-YaoFang on Cl− and HCO3- Transport in Diarrhea-Predominant Irritable Bowel Syndrome Rats

    Directory of Open Access Journals (Sweden)

    Xiaofang Lu

    2016-01-01

    Full Text Available TongXie-YaoFang (TXYF can effectively alleviate the symptoms of diarrhea-predominant irritable bowel syndrome (D-IBS patients. However, the curative mechanism has not been fully clarified. The study was designed to investigate the effect of TXYF on the colonic ion transport induced by serotonin (5-HT in D-IBS rats. A method of multiple stress (neonatal maternal separation (NMS combined with restraint stress (RS was used to induce the D-IBS model. The model rats were randomly divided into two groups: NMS + RS group and TXYF-formula group, and the normal control (no handling rats were classified as NH group. In the NMS + RS group, the change of short-circuit current (ΔIsc induced by 5-HT was lower than that in the NH and TXYF-formula groups. After removing of the extracellular Cl− or HCO3- or basolateral Na+ or blocking the cystic fibrosis transmembrane conductance regulator (CFTR, Na+-K+-2Cl− cotransporter (NKCC, Na+-HCO3- cotransporter, Cl-/HCO3- exchanger, K+ channel, or Na+/K+-ATPase, respectively, there was no difference in 5-HT-induced ΔIsc among the three groups. These data suggest that TXYF can regulate 5-HT-induced Cl− and HCO3- secretion, possibly mediated by the combined action of CFTR, NKCC, Na+-HCO3- cotransporter, Cl-/HCO3- exchanger, K+ channel, and Na+/K+-ATPase.

  17. 大渡裸裂尻鱼资源现状与保护措施%Distribution Status and Protection Measure of Schizopygopsis Malacanthus Chengi (Fang)

    Institute of Scientific and Technical Information of China (English)

    李华; 李强; 李联满

    2012-01-01

      Schizopygopsis malacanthus chengi (Fang) are principally distributed in the upper reaches of the Dadu River, which is endemic species in China. The population of the fish living in the upper reaches of the Dadu River has gone down quickly so far, The reasons accountable for the decline of the fish resources are due to water conservancy project, river mining and overfishing. The fish can be proliferated and protected by enhancing fishery management, reinforcing scientific research, and carrying out the return of the individuals cultured.%  大渡裸裂尻鱼主要分布在大渡河水系上游河段,属于我国的特有鱼类。目前大渡裸裂尻鱼在大渡河水系上游的资源量在急剧下降,造成这种现象的原因与水利工程、河道采矿和过度捕捞等因素有关。可通过强化渔政管理、加强科学研究以及采取人工增殖放流等主要措施,对大渡裸裂尻鱼进行增殖保护。

  18. Greetings: Professor Pei-Ming Ho won the 2011 AAA Award and Prof.Cenke Xu won the 2011 OYRA Award

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Professor Pei-Ming Ho won the 2011 Achievement in Asia Award (AAA),and Prof.Cenke Xu won the 2011 Outstanding Young Researcher Award (OYRA) of the Overseas Chinese Physics Association (OCPA).The OCPA AAA Award is given annually to Chinese physicists working in Asia in recognition of their outstanding achievements in physics,and the OYRA Award is given annually to a physicist of Chinese ethnicity working in North America,Europe,or other regions outside Asia,to encourage and to recognize the young researcher's contributions in physics.

  19. Preparation Gold-PEI Nanoparticles for Gene Delivery%纳米金-聚乙烯亚胺基因载体的制备

    Institute of Scientific and Technical Information of China (English)

    卢耀勇; 王冬晓; 王建国; 张霞; 王莺

    2013-01-01

    目的:基因方法治疗癌症近年来取得了很大的突破,因此基因载体的构建显得尤为重要.其中纳米基因载体合成简单,成本低廉,并能够包裹、浓缩、保护核苷酸使其免受核酸酶降解,因此纳米材料广泛地应用于基因输送.我们拟开展聚乙烯亚胺-纳米金基因载体的制备及其表征.方法:采用层层包裹技术制备基因载体,首先通过柠檬酸钠还原法制备纳米金颗粒后,应用11-巯基十一烷酸对金颗粒进行修饰,使其表面带有羧基,然后进一步将带有氨基的低分子量聚乙烯亚胺与羧基进行连接.应用动态光散射(DLS),紫外可见光谱(UV)和透射电子显微镜(TEM)对构建的纳米基因载体进行表征.结果:成功制备了聚乙烯亚胺-纳米金基因载体,检测表明每一步制备出的产物纳米尺寸在20-30nm之间,液体均匀稳定,分散系数(PDI)在0.2以下,Zeta电位测定表明,每步的产物电荷变化与外层包裹的反应物有关.尽管金颗粒外层包裹聚乙烯亚胺,但是总体上纳米载体尺寸没有发生太大的变化,TEM检测表明每一步形成了均匀的、单分散的、球状的纳米颗粒.结论:我们通过层层包裹技术成功制备了聚乙烯亚胺-纳米金基因载体,在进一步开展的生物活性的检测中,希望通过纳米载体的携带作用,将基因转染进靶细胞,从而检测相关基因对靶细胞的沉默作用,提高基因药物的应用,为开发新型基因药物提供基础.%Objective:In recent years,gene delivery system has made great breakthrough in cancer therapy,therefore it is important to fabricate efficient gene carrier.Among them,nanomaterials which can entrap and concentrate nucleic acid thereby avoiding degradation by nuclease have been extensively used in gene delivery due to their easy preparation and low cost.Herein,we prepared and characterized gold-polyethyleneimine (Gold-PEI) nanopaticles for gene delivery.Methods:We demonstrated a

  20. Long-term results of interventional treatment of large unresectable hepatocellular carcinoma (HCC): significant survival benefit from combined transcatheter arterial chemoembolization (TACE) and percutaneous ethanol injection (PEI) compared to TACE monotherapy; Langzeitergebnisse der interventionellen Therapie von grossen, inoperablen hepatozellulaeren Karzinomen (HCC): signifikanter Ueberlebensvorteil von transarterieller Chemoembolisation (TACE) und perkutaner Ethanolinjektion (PEI) gegenueber der TACE-Monotherapie

    Energy Technology Data Exchange (ETDEWEB)

    Lubienski, A.; Bitsch, R.G.; Grenacher, L.; Kauffmann, G.W. [Radiologische Universitaetsklinik Heidelberg, Abt. Radiodiagnostik, Heidelberg (Germany); Schemmer, P. [Chirurgische Universitaetsklinik Heidelberg (Germany); Duex, M. [Radiologisches Zentralinstitut Krankenhaus Nordwest Frankfurt (Germany)

    2004-12-01

    Purpose: A retrospective analysis of long-term efficacy of combined transcatheter arterial chemoembolization (TACE) and percutaneous ethanol injection (PEI) and TACE monotherapy was conducted in patients with large, non-resectable hepatocellular carcinoma (HCC). Methods and Materials: Fifty patients with large, unresectable HCC lesions underwent selective TACE. Liver cirrhosis was present in 42 patients, due to alcohol abuse (n = 22) and viral infection (n = 17). In three patients, the underlying cause for liver cirrhosis remained unclear. Child A cirrhosis was found in 22 and Child B cirrhosis in 20 patients. Repeated and combined TACE and PEI were performed in 22 patients and repeated TACE monotherapy was performed in 28 patients. Survival and complication rates were determined and compared. Results: The 6-, 12-, 24- and 36-month survival rates were 61%, 21%, 4%, and 4% for TACE monotherapy and 77%, 55%, 39% and 22% for combined TACE and PEI (Kaplan-Meier method). The kind of treatment significantly affected the survival rate (p=0.002 log-rank test). Severe side effects were present in two patients of the monotherapy group and in three patients of the combination therapy group. (orig.)

  1. A comparison of the effectiveness of cationic polymers poly-L-lysine (PLL) and polyethylenimine (PEI) for non-viral delivery of plasmid DNA to bone marrow stromal cells (BMSC).

    Science.gov (United States)

    Farrell, Laura-Lee; Pepin, Joel; Kucharski, Cezary; Lin, Xiaoyue; Xu, Zhenghe; Uludag, Hasan

    2007-03-01

    Bone marrow stromal cells (BMSC) represent an important cell phenotype for pursuit of successful gene therapy. Non-viral methods to enable expression of exogenous genes in BMSC will accelerate clinical application of gene therapy, without the concerns associated with the viral means of gene transfer. Towards this end, this study investigated the potential of cationic polymers poly-L-lysine (PLL) and branched polyethylenimine (PEI) as gene carriers for modification of BMSC. Both polymers rapidly (approximately 30 min) condensed a 4.2 kb Enhanced Green Fluorescent Protein (pEGFP-N2) plasmid into 100-200 nm particles. PLL and PEI were both readily internalized with BMSC with >80% of BMSC exhibiting polymer uptake by flow cytometric analysis. The relative uptake of PEI, however, was significantly higher as compared to the PLL. The majority of the BMSC (>60%) exhibited nuclear presence of the polymers as analyzed by fluorescent microscopy. Although both polymers were able to deliver the pEGFP-N2 into the cells under microscopic evaluation, only a small fraction of the cells (PLL and PEI readily displayed cellular uptake, but PEI was more effective in delivering plasmid DNA intracellularly, which was likely the underlying basis for a more sustained gene expression.

  2. Efficacious cellular codelivery of doxorubicin and EGFP siRNA mediated by the composition of PLGA and PEI protected gold nanoparticles.

    Science.gov (United States)

    Kumar, Krishan; Vulugundam, Gururaja; Jaiswal, Pradeep Kumar; Shyamlal, Bharti Rajesh Kumar; Chaudhary, Sandeep

    2017-09-15

    This study reports the simultaneous delivery of EGFP siRNA and the chemotherapeutic drug, doxorubicin by means of the composition that results from the electrostatic interaction between positively charged siRNA-complexes of gold nanoparticles (AuNPs) capped with PEI, 25kDa (P25-AuNPs) and negatively charged carboxymethyl cellulose formulated PLGA nanoparticles loaded with doxorubicin. The nanoparticles and their facile interaction were studied by means of dynamic light scattering (DLS), zeta potential, transmission electron microscopic (TEM) measurements. The flow cytometric and confocal microscopic analysis evidenced the simultaneous internalization of both labelled siRNA and doxorubin into around 55% of the HeLa cancer cell population. Fluorescence microscopic studies enabled the visual analysis of EGFP expressing HeLa cells which suggested that the composition mediated codelivery resulted in a substantial downregulation of EGFP expression and intracellular accumulation of doxorubicin. Interestingly, codelivery treatment resulted in an increased cellular delivery of doxorubicin when compared to PLGA-DOX alone treatment. On the other hand, the activity of siRNA complexes of PEI-AuNPs was completely retained even when they were part of composition. The results suggest that this formulation can serve as promising tool for delivery applications in combinatorial anticancer therapy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Programa de Enriquecimiento Instrumental (PEI: alternativa pedagógica que responde al desafío de calidad en educación

    Directory of Open Access Journals (Sweden)

    Aida Varela

    2006-01-01

    Full Text Available Este artículo presenta la implementación del Programa de Enriquecimento Instrumental en las Escuelas de Enseñanza Secundaria de la red pública estatal de bahia, experiencia acompañada por un proyecto de investigación delineado para evaluar la efectividad del programa como aplicación de la teoría de la modificabilidad cognitiva estructural. la metodología usada fue la experiencia de aprendizaje mediado, en lo que se refiere alproceso de asimilación, significación y transferencia /trascendencia de información por parte de profesores y alumnos. La investigación se ha constituido en un estudio comparativo entre alumnos de unidades escolares que ya integran el PEI a su currículo (grupo experimental y de aquellas que no tienen la investigación del PEI(grupo control. El análisis de los datos revela modificabilidad en el proceso enseñanza-aprendizaje.

  4. 葡萄糖淀粉酶在高压静电纺PEI/PVA纳米纤维膜上的离子吸附固定%Immobilization of Glucoamylase onto the Electrospinning PEI/PVA Composite Nanofiber Membrane via Ionic Adsorption

    Institute of Scientific and Technical Information of China (English)

    隋春红; 王程; 董顺福; 韩丽琴

    2015-01-01

    旨在应用离子结合法将葡萄糖淀粉酶固定在PEI/PVA纳米纤维膜上并对其理化性质进行研究。采用高压静电纺丝技术制备聚乙烯亚胺(PEI)/聚乙烯醇(PVA)纳米纤维膜,采用热交联方法使其具备水稳定性后,再利用离子吸附法固定葡萄糖淀粉酶。结果显示,利用红外光谱(FT-IR)表征固定有葡萄糖淀粉酶的PEI/PVA纳米纤维膜,表明葡萄糖淀粉酶可成功固定在静电纺丝形成的PEI/PVA纳米纤维膜表面。通过固定化葡萄糖淀粉酶的酶学性质鉴定,发现固定化葡萄糖淀粉酶的最适反应温度为65℃,比游离的葡萄糖淀粉酶提高了6℃;固定化葡萄糖淀粉酶的适用pH值范围明显变宽;热稳定性和存贮稳定性显著增强且可以重复使用。利用离子吸附法能简便地将蛋白质分子固定于纳米纤维膜上,具有一定的应用前景。%It was to study the physical and chemical properties of immobilization of glucoamylase on the PEI/PVA nanofibers via ion bonding. Polyethyleneimine(PEI)/polyvinyl alcohol(PVA)composite nanofiber membrane was prepared by electrospinning technology, and thermal crosslinking was applied to make it possess water stability. Glucoamylase was immobilized onto the electrospinning PEI/PVA composite nanofiber membrane via ion adsorption. Results showed that fourier transform infrared spectrum(FT-IR)indicated that glucoamylase can be successfully fixed on the surface of the electrospinning PEI/PVA composite nanofiber membrane. Then, the enzymatic properties of immobilized glucoamylase were identified. The results have shown that the optimum reaction temperature of immobilized glucoamylase is 65℃, 6 degrees higher than free glucoamylase, and the applicable pH range of immobilized glucoamylase is wider than free glucoamylase significantly. In addition, immobilized glucoamylase also has good thermal stability, storage stability and reusability. The use of ion

  5. Anatomy Features and Environment Flexibility of Rhododendron shanii Fang%都支杜鹃茎、叶解剖特征与环境的适应性

    Institute of Scientific and Technical Information of China (English)

    王桂芹; 刘艳然

    2012-01-01

    利用光学显微解剖技术,对生长于大别山区的都支杜鹃(Rhododendron shanii Fang)的茎、叶进行了解剖学与环境适应性之间的关系研究.结果表明:叶的上表皮具有厚的角质膜、复表皮、气孔缺失;下表皮被毛,气孔分布密集,气孔突出;叶肉中栅栏组织和纤维丰富,且纤维呈束存在,并形成了叶肉中的支撑系统,海绵组织内具有发达的通气组织;茎中木栓宽广,次生韧皮部与次生木质部厚度比是1/5,次生木质部中管孔直径狭窄、管密孔集,绝大多数为管胞,同时木纤维十分丰富.上述结构的特异性均表明茎和叶在诸多方面表现为结构与环境的统一性,是特殊综合生态环境长期作用的结果.复表皮、主脉周韧维管束、次生木质部缺少导管等这些特征均说明都支杜鹃在系统分类中应处于较原始的地位.%The relationships between the anatomy and the adaptation to the environment for stems and leaves of Rhododendron shanii Fang growing in the Dabie Mountain area were studied by means of optical microdissection technology. The results show that there are thick cuticle and multiple epidermis and stomatal absence on the leaves' epidermis; there are hair and distribution of prominent stomatal are serried under epidermis of leaves; there are abundance of palisade tissue and fibre in mesophyll, the fibre constitutes fiber bundle forming the skeleton system of mesophyll. Cork is wide in stem, the thickness ratio is 1/5 between the secondary phloem and secondary xylem; Most of pores with pores diameter stenosis and over disperse are the tracheid and moreover fibre is very rich in secondary xylem. All these features indicate that stems and leaves have unity of structure and environment in many aspects, such structural features results from adapting to environments. These features with multiple epidermis and amphicribral bundle of the main vein and vessel deficiency etc suggests that R. shanii is

  6. Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice.

    Science.gov (United States)

    Li, Jinyu; Wei, Yi; Li, Xue; Zhu, Dashuai; Nie, Bo; Zhou, Jingwei; Lou, Lixia; Dong, Bin; Wu, Aiming; Che, Yongzhe; Chen, Meng; Zhu, Lingqun; Mu, Mingwei; Chai, Limin

    2017-01-05

    Xian-Fang-Huo-Ming-Yin (XFHM), a traditional herbal formula, has been used to treat sores and carbuncles for hundreds of years in Asia. Nowadays, its clinical effects in treatment of rheumatoid arthritis (RA) have been validated. In this study, we want to study its possible molecular mechanisms of regulating the differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis (CIA) mice for RA treatment. A high performance liquid chromatography-electrospray ionization/mass spectrometer (HPLC-ESI/MS(n)) system was used to analyze the constituents of XFHM granules. An arthritics mouse model was induced by collagen and leflunomide (LEF) was used as a positive control medicine. Pathological changes at the metatarsophalangeal joint were studied through Safranin O and immunohistochemical staining. The differentiation of T, B and NK cells was examined by flow cytometry and pro-inflammatory cytokines were assayed using an Inflammation Antibody Array assay. The expression of key molecules of the nuclear factor κB (NF-κB) and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathways in spleen were studied by western-blot analysis. In our study. 21 different dominant chemical constituents were identified in XFHM. Treatment with XFHM suppressed the pathological changes in arthrosis of CIA. Additionally, XFHM down-regulated the proliferation and differentiation of CD3(+) T cells and CD3(-)CD19(+) B cells significantly. However, XFHM had no significant effect on CD3(-)NK1.1(+) NK cells. Further study showed that the production of pro-inflammatory cytokines had been suppressed by inhibiting the activation of NF-κB and JAK/STAT signaling. XFHM can regulate and maintain the immunologic balance of lymphocytic immunity and inhibit the production of pro-inflammatory cytokines, thus suppressing the pathological changes of RA. Therefore, XFHM may be used as an application of traditional medicine against RA

  7. 《救迷良方》与戒除鸦片烟毒%Jiu mi liang fang (Effective Prescriptions for Rescuing Addict) and quitting from opium addiction

    Institute of Scientific and Technical Information of China (English)

    刘悦

    2008-01-01

    清代青浦著名医家何其伟,在鸦片烟毒泛滥、严重危害人民健康之际,应林则徐要求,秉着医家的责任感和同情心,辑录戒烟方论而成.这部现存最早的戒烟专著,理法方药齐备,对鸦片的危害、性味归经,瘾成过程及病机、瘾发症状等,均有较全面、正确的认识,指出戒烟需先立志坚定,以治心瘾,所载戒烟方药,经过了实践验证,当时流传甚广;所述戒烟思想和方法,一直为后世沿用,在民间广泛传播.%When opium was running rampantly and seriously damaging people's health in the Qing dynasty,in response to the request of Lin Ze-xu,He Qi-wei,a contemporary famous physician at Qingpu,based on his own professional responsibility and compassion,compiled Jiu mi liang fang (Effective Prescriptions for Rescuing Addict),the first extant monograph on quitting opium which includes the detriments of opium,its nature,taste and channel tropism,causes and pathogenesis of addiction,and the symptoms.He pointed out that the powerful will was essential.The prescriptions recorded in the book were effective through the proof of practice and very popular at that time.The thought and measures he mentioned are stillin use even now,and spread widely in the folks.

  8. Chemical profiles and pharmacological activities of Chang-Kang-Fang, a multi-herb Chinese medicinal formula, for treating irritable bowel syndrome.

    Science.gov (United States)

    Mao, Qian; Shi, Lei; Wang, Zhi-Gang; Luo, Yu-Hui; Wang, Yin-Yu; Li, Xue; Lu, Min; Ju, Jian-Min; Xu, Jin-Di; Kong, Ming; Zhou, Shan-Shan; Shen, Min-Qin; Li, Song-Lin

    2017-04-06

    Chang-Kang-Fang formula (CKF), a multi-herb traditional Chinese medicinal formula, has been clinically used for treatment of irritable bowel syndrome (IBS). The mechanisms of CKF for treating IBS and the components that are responsible for the activities were still unknown. To investigate the chemical profiles and effects of CKF on IBS model. The chemical profiles of CKF were investigated by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q/TOF-MS/MS). On colon irritation induced rat neonates IBS model, the influence of CKF on neuropeptides, including substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal polypeptide (VIP) and 5-hydroxytryptamine (5-HT), were measured by ELISA, and the effect on intestinal sensitivity was assessed based on the abdominal withdrawal reflex (AWR) scores. In addition, the activities of CKF against acetic acid-induced nociceptive responses and prostigmin methylsulfate triggered intestinal propulsion in mice were also evaluated. 80 components were identified or tentatively assigned from CKF, including 11 alkaloids, 20 flavanoids, 4 monoterpenoids, 9 iridoid glycoside, 9 phenylethanoid glycosides, 10 chromones, 7 organic acid, 3 coumarins, 2 triterpene and 5 other compounds. On IBS rat model, CKF was observed to reduce AWR scores and levels of SP, CGRP, VIP and 5-HT. Moreover, CKF reduced the acetic acid-induced writhing scores at all dosages and reduced the intestinal propulsion ration at dosage of 7.5 and 15.0g/kg/d. CKF could alleviate the symptoms of IBS by modulating the brain-gut axis through increasing the production of neuropeptides such as CGRP, VIP, 5-HT and SP, releasing pain and reversing disorders of intestinal propulsion. Berberine, paeoniflorin, acteoside, flavonoids and chromones may be responsible for the multi-bioactivities of CKF. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  9. San Pascual (1991) Año XXVIII, n. 284

    OpenAIRE

    Pérez, María Dolores, O.S.C. (Directora)

    1991-01-01

    Editorial. Documento de la Santa sede. San Pascual y la Virgen de Gracia. Nueva revista dedicada a San Pascual. Adoración nocturna. Desde la clausura. Vida en el santuario de San Pascual. Orden Franciscana. Coplas a San Pascual Baylón. San Pascual en el arte. Rutas pascualinas. A l'ombra del claustre.

  10. San Pascual (1991) Año XXVIII, n. 284

    OpenAIRE

    Pérez, María Dolores, O.S.C. (Directora)

    1991-01-01

    Editorial. Documento de la Santa sede. San Pascual y la Virgen de Gracia. Nueva revista dedicada a San Pascual. Adoración nocturna. Desde la clausura. Vida en el santuario de San Pascual. Orden Franciscana. Coplas a San Pascual Baylón. San Pascual en el arte. Rutas pascualinas. A l'ombra del claustre.

  11. Species - San Diego Co. [ds121

    Data.gov (United States)

    California Department of Resources — This is the Biological Observation Database point layer representing baseline observations of sensitive species (as defined by the MSCP) throughout San Diego County....

  12. 33 CFR 165.1102 - Security Zone; Naval Base Point Loma; San Diego Bay, San Diego, CA.

    Science.gov (United States)

    2010-07-01

    ... Loma; San Diego Bay, San Diego, CA. 165.1102 Section 165.1102 Navigation and Navigable Waters COAST... Guard District § 165.1102 Security Zone; Naval Base Point Loma; San Diego Bay, San Diego, CA. (a) Location. The following area is a security zone: The water adjacent to the Naval Base Point Loma, San Diego...

  13. 77 FR 57494 - Safety Zone; Fleet Week Fireworks, San Francisco Bay, San Francisco, CA

    Science.gov (United States)

    2012-09-18

    ... SECURITY Coast Guard 33 CFR Part 165 Safety Zone; Fleet Week Fireworks, San Francisco Bay, San Francisco... will enforce the safety zone for the Fleet Week Fireworks in the Captain of the Port, San Francisco...'' W (NAD83) for the Fleet Week Fireworks in 33 CFR 165.1191, Table 1, item number 25. This safety...

  14. 78 FR 10062 - Safety Zone; Sea World San Diego Fireworks, Mission Bay; San Diego, CA

    Science.gov (United States)

    2013-02-13

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Sea World San Diego Fireworks, Mission Bay; San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone on the navigable waters of Mission Bay in support of the Sea World San Diego...

  15. 77 FR 42649 - Safety Zone: Sea World San Diego Fireworks, Mission Bay; San Diego, CA

    Science.gov (United States)

    2012-07-20

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone: Sea World San Diego Fireworks, Mission Bay; San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is... rule, call or email Petty Officer David Varela, Waterways Management, U.S. Coast Guard Sector San Diego...

  16. 77 FR 60899 - Safety Zone; Sea World San Diego Fireworks, Mission Bay; San Diego, CA

    Science.gov (United States)

    2012-10-05

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Sea World San Diego Fireworks, Mission Bay; San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone on the navigable waters of Mission Bay in support of the Sea World San Diego...

  17. 77 FR 42638 - Safety Zone: Sea World San Diego Fireworks, Mission Bay; San Diego, CA

    Science.gov (United States)

    2012-07-20

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone: Sea World San Diego Fireworks, Mission Bay; San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone on the navigable waters of Mission Bay in support of the Sea World San Diego...

  18. 78 FR 29025 - Sea World San Diego Fireworks 2013 Season; Mission Bay, San Diego, CA

    Science.gov (United States)

    2013-05-17

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Sea World San Diego Fireworks 2013 Season; Mission Bay, San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone on the navigable waters of Mission Bay in support of the Sea World San Diego...

  19. 76 FR 46352 - Approval of Noise Compatibility Program for San Diego International, San Diego, CA

    Science.gov (United States)

    2011-08-02

    ... Federal Aviation Administration Approval of Noise Compatibility Program for San Diego International, San Diego, CA AGENCY: Federal Aviation Administration, DOT. ACTION: Notice . SUMMARY: The Federal Aviation Administration (FAA) announces its findings on the noise compatibility program submitted by San Diego Regional...

  20. 78 FR 77597 - Safety Zone; Allied PRA-Solid Works, San Diego Bay; San Diego, CA

    Science.gov (United States)

    2013-12-24

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Allied PRA-Solid Works, San Diego Bay; San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the navigable waters of the San Diego Bay in support of a fireworks...

  1. 桂枝解郁方不同工艺抗抑郁作用的比较%Comparison on Anti-depressant Effects of Guizhi Jieyu Fang by Different Techniques

    Institute of Scientific and Technical Information of China (English)

    龚梦鹃; 何伟; 唐春萍; 杨超艳

    2011-01-01

    目的:比较桂枝解郁方不同工艺的抗抑郁作用.方法:本实验将小鼠随机分成4组,即桂枝解郁方-A组、桂枝解郁方-B组(分别ig给予桂枝解郁液-A和桂枝解郁液-B 12.66 g·kg-1,每天1次,连续给药10 d)、盐酸阿米替林组(实验前3 dig给予盐酸阿米替林50 mg·kg-1,每天1次)、空白对照组(给予等体积的生理盐水).采用小鼠强迫游泳实验、悬尾实验、自主活动实验和利血平拮抗实验,以小鼠不动时间、自主活动数和体温下降值为指标来评价桂枝解郁方不同工艺抗抑郁的作用.结果:在强迫游泳实验和悬尾实验中,桂枝解郁方-A能明显缩短小鼠的不动时间(P<0.05);在自主活动实验中,各组小鼠之间无显著性差异.利血平拮抗实验中,桂枝解郁方-A在4h能显著拮抗利血平诱发的小鼠体温降低(P<0.05).结论:桂枝解郁方-A的抗抑郁作用优于桂枝解郁方B.%Objective: To compare the anti-depressant effects of Guizhi Jieyu Fang by different techniques.Method: Mice were divided randomly into four groups: two treatment groups(Guizhi Jieyu Fang-A and Guizhi Jieyu Fang-B orally given for 10 days, 12.66 g· kg -1, daily ), amitriptyline hydrochloride group ( amitriptyline hydrochloride orally given for 3 days 50 g· kg- 1, daily) and control group. The forced swimming test, tail suspension test, open field activity test and reserpine-induced hypothermia test were carried out to determine the antidepressant activity in mice. Mice immobility time in forced swimming test and tail suspension test,spontaneous activity frequency in open field activity test, and body temperature decrease in reserpine-induced hypothermia test were observed. Result:Guizhi Jieyu Fang-A could significantly shorten immobility time in forced swimming test and tail suspension test and inhabit the decrease of body temperature in reserpine antagonistic test at the 4th hour( P <0.05 ) ,but no impact in open field activity test

  2. Cuartel San Carlos. Yacimiento veterano

    Directory of Open Access Journals (Sweden)

    Mariana Flores

    2007-01-01

    Full Text Available El Cuartel San Carlos es un monumento histórico nacional (1986 de finales del siglo XVIII (1785-1790, caracterizado por sufrir diversas adversidades en su construcción y soportar los terremotos de 1812 y 1900. En el año 2006, el organismo encargado de su custodia, el Instituto de Patrimonio Cultural del Ministerio de Cultura, ejecutó tres etapas de exploración arqueológica, que abarcaron las áreas Traspatio, Patio Central y las Naves Este y Oeste de la edificación. Este trabajo reseña el análisis de la documentación arqueológica obtenida en el sitio, a partir de la realización de dicho proyecto, denominado EACUSAC (Estudio Arqueológico del Cuartel San Carlos, que representa además, la tercera campaña realizada en el sitio. La importancia de este yacimiento histórico, radica en su participación en los acontecimientos que propiciaron conflictos de poder durante el surgimiento de la República y en los sucesos políticos del siglo XX. De igual manera, se encontró en el sitio una amplia muestra de materiales arqueológicos que reseñan un estilo de vida cotidiana militar, así como las dinámicas sociales internas ocurridas en el San Carlos, como lugar estratégico para la defensa de los diferentes regímenes que atravesó el país, desde la época del imperialismo español hasta nuestros días.

  3. Downtown revitalization in San Salvador

    OpenAIRE

    Ejeborn, Elisabet; Nedersjö, Julia

    2012-01-01

    The aim of this master’s thesis in spatial planning is to research the conditions in the historic city centre of San Salvador and make a strategy and urban design proposal for the area, but also to investigate the relationship between economic development, public institutions and the public space in this area. The research has been done through literature studies on El Salvador and formal/informal economy, onsite inventory, studies of good examples and interviews with people in the area. In t...

  4. Downtown revitalization in San Salvador

    OpenAIRE

    Ejeborn, Elisabet; Nedersjö, Julia

    2012-01-01

    The aim of this master’s thesis in spatial planning is to research the conditions in the historic city centre of San Salvador and make a strategy and urban design proposal for the area, but also to investigate the relationship between economic development, public institutions and the public space in this area. The research has been done through literature studies on El Salvador and formal/informal economy, onsite inventory, studies of good examples and interviews with people in the area. In t...

  5. The Characteristics and Significance of Using Allusions In FangWeng Ci-Poem%放翁词中典故运用的特征及其意义

    Institute of Scientific and Technical Information of China (English)

    邢蕊杰

    2011-01-01

    Ci-Poem Using allusions is very popular in South-Song Dynasty. Lu you and Xin qi-ji are the represen- tative writers. Lu you, also named Fang weng, has lots of allusions in his Ci-Poem, which involve the different meaning of history and profound implications. Therefore FangWeng tries to use various ways to express the allu- sions in his Ct-Poem, which as a true portrayal of his inner world, helps him to reflect his life experience and ideas truly. Using allusions in the FangWeng Ci-Poem has the lyric and flexible characteristics, which enhances the elegant and dignified temperament to Ci-Poem. Using allusions reflect the current aesthetic fashion in Song Dy- nasty and also is the result of the Ci-Poem evolution.%词的用典,在南宋词坛渐成风气,以陆游、辛弃疾为代表。陆放翁以典入词,数量多、范围广,熔铸百家,广博精当。放翁词语典内容广泛,事典意蕴深厚,用法灵活多样。典故中的历史积淀形象表达了词人的人生情怀或思想志趣,成为其内心世界的真实写照。放翁词大量用典,抒情言志,增强了词作的书卷气,增添了其风雅相,是时代审美风尚的具体反映以及词体演进的必然结果。

  6. 旱地脂脱方联合梅花针叩刺治疗脂溢性脱发疗效观察%Observation of Han Di Zhi Tuo Fang Combined with Percussopunctator in Treating Alopecia Seborrhoeica

    Institute of Scientific and Technical Information of China (English)

    李恒

    2014-01-01

    目的:观察旱地脂脱方联合梅花针叩刺治疗脂溢性脱发的治疗效果。方法选择86例脂溢性脱发患者随机分为观察组和对照组各43例;对照组给予口服维生素B6片剂治疗,治疗组给予旱地脂脱方联合梅花针叩刺治疗。比较两组治疗效果。结果治疗组总有效率(88.37%)高于对照组(74.42%),差异有统计学意义(P<0.05)。结论旱地脂脱方联合梅花针叩刺治疗脂溢性脱发疗效显著,值得进一步推广。%ObjectTo analysis the effect of Han Di Zhi Tuo Fang combined with percussopunctator in treating alopecia seborrhoeica. Methods: 86 cases of alopecia seborrhoeica were divided into treatment group and control group with 43 cases. Control group were treated by Vitamin B6. Treatment group were treated with Han Di Zhi Tuo Fang combined with percussopunctator. The clinical effects of the two groups were observed. Results: The total effective rate in treatment group (88.37%) was significantly higher than control group (74.42%), the difference was statistical significance (p<0.05). Conclusion:The therapy of Han Di Zhi Tuo Fang combined with percussopunctator has a good effect in treating alopecia seborrhoeica, worth clinical promotion.

  7. Treating 2 cases of MDS by Professor PEI Zheng-xue%裴正学教授治愈MDS 2例

    Institute of Scientific and Technical Information of China (English)

    陈光艳; 靖芳

    2014-01-01

    骨髓增生异常综合征(Myelodysplastic Syndromes,MDS)病因不明,发病机理不清,西医治疗无策。裴正学教授具有多年的临床实践经验,擅长治疗各种疑难病症,其以“兰州方”治疗MDS 2例,疗效显著。%MDS unknown etiology, pathogenesis is unclear, modern medicine is no policy. Professor PEI Zheng-xue has many years of clinical experience, specializes in treating various incurable diseases, using the “Lanzhou recipe” for treating MDS 2 cases, a significant effect.

  8. Effects of SongYuAnShenFang on Serum Cytokines Levels of Sleep Dep-rivation Rats%松郁安神方对睡眠剥夺大鼠血清细胞因子的影响

    Institute of Scientific and Technical Information of China (English)

    李星; 黄俊山; 张一帆; 曾雪爱; 张娅

    2014-01-01

    OBJECTIVE To observe the effects of SongYuAnShenFang on serum cytokines levels of sleep depri-vation rats.METHODS The sleep deprivation in rats model was constructed with the method called “modified mul-tiple platform method”.Comparions were housed either in normal cages or in tanks with large platforms.After the treatment of SongYuAnShenFang in Sleep Deprivation Rats ,serum levels of IL-1β、TNF-αwere measured by enzyme linked immunosorbent assay.RESULTS Significant differences in serum level of TNF-αwere observed between SongYuAnShenFang group and REM sleep deprivation group ( P0.05 ).CONCLUSION SongYuAnShenFang in improving sleep quality may be based on its regulation of serum level of TNF-α,then adjust the excessive body metabolism caused by sleep depriveation.%目的:观察松郁安神方对睡眠剥夺大鼠血清细胞因子的影响,进一步探讨松郁安神方调节睡眠的机制。方法采用“改良多平台睡眠剥夺法”构建睡眠剥夺大鼠模型,以正常笼养及大平台作为对照组,采用酶联免疫吸附法测定睡眠剥夺大鼠经松郁安神方治疗后,血清细胞因子IL-1β、TNF-α水平的变化。结果松郁安神方组血清TNF-α高于REM睡眠剥夺组(P<0.05);松郁安神方组血清IL-1β较正常对照组、大平台环境对照组及REM睡眠剥夺组无显著性差异(P>0.05)。结论松郁安神方可能通过调节血清细胞因子TNF-α的含量从而调节睡眠,且通过对睡眠剥夺大鼠睡眠的调节继而改善睡眠剥夺所致机体的过度能量代谢。

  9. Evaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal models of hemorrhagic spinal cord injury.

    Science.gov (United States)

    Liu, Yu; He, Zhi-Jie; Xu, Bo; Wu, Qi-Zhu; Liu, Gang; Zhu, Hongyan; Zhong, Qian; Deng, David Y; Ai, Hua; Yue, Qiang; Wei, Yi; Jun, Shen; Zhou, Guangqian; Gong, Qi-Yong

    2011-05-19

    Cell tracking using iron oxide nanoparticles has been well established in MRI. However, in experimental rat models, the intrinsic iron signal derived from erythrocytes masks the labeled cells. The research evaluated a clinically applied Gd-DTPA for T1-weighted positive enhancement for cell tracking in spinal cord injury (SCI) rat models. MSCs were labeled with jetPEI/Gd-DTPA particles to evaluate the transfection efficiency by MRI in vitro. Differentiation assays were carried out to evaluate the differentiation ability of Gd-DTPA-labeled MSCs. The Gd-DTPA-labeled MSCs were transplanted to rat SCI model and monitored by MRI in vivo. Fluorescence images were taken to confirm the MRI results. Behavior test was assessed with Basso, Beattie, and Bresnahan (BBB) scoring in 6weeks after cell transplantation. The Gd-labeled MSCs showed a significant increase in signal intensity in T1-weighted images. After local transplantation, Gd-DTPA-labeled MSCs could be detected in SCI rat models by the persistent T1-weighted positive enhancement from 3 to 14days. Under electronic microscope, Gd-DTPA/jetPEI complexes were mostly observed in cytoplasm. Fluorescence microscopy examination showed that the Gd-labeled MSCs survived and distributed within the injured spinal cord until 2weeks. The Gd-labeled MSCs were identified and tracked with MRI by cross and sagittal sections. The BBB scores of the rats with labeled MSCs transplantation were significantly higher than those of control rats. Our results demonstrated that Gd-DTPA is appropriate for cell tracking in rat model of SCI, indicating that an efficient and nontoxic label method with Gd-DTPA could properly track MSCs in hemorrhage animal models. Copyright © 2011 Elsevier B.V. All rights reserved.

  10. Trouble Brewing in San Francisco. Policy Brief

    Science.gov (United States)

    Buck, Stuart

    2010-01-01

    The city of San Francisco will face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that San Francisco faces an aggregate $22.4 billion liability for pensions and retiree health benefits that are underfunded--including $14.1 billion for the city…

  11. Induction of a balanced Th1/Th2 immune responses by co-delivery of PLGA/ovalbumin nanospheres and CpG ODNs/PEI-SWCNT nanoparticles as TLR9 agonist in BALB/c mice.

    Science.gov (United States)

    Ebrahimian, Mahboubeh; Hashemi, Maryam; Maleki, Mohsen; Abnous, Khalil; Hashemitabar, Gholamreza; Ramezani, Mohammad; Haghparast, Alireza

    2016-10-29

    To develop effective and safe vaccines with reduced dose of antigen and adjuvant, intelligent delivery systems are required. Many delivery systems have been developed to enhance the biological activity of cytosine-phosphorothioate-guanine oligodeoxynucleotides (CpG ODN) as both immunotherapeutic agents and vaccine adjuvants. In this study we designed a novel CpG ODN delivery system based on single-walled carbon nanotube (SWCNT) functionalized with polyethylenimine (PEI) and alkylcarboxylated PEI (AL-PEI). The physicochemical characteristics, cytotoxicity and cellular uptake studies of these carriers were performed. All carriers were conjugated with CpG ODN followed by co-delivery with ovalbumin (OVA) encapsulated into poly (lactic-co-glycolic acid) nanospheres (PLGA NSs) to enhance the induction of immune responses. The effect of these formulations on antibody (IgG1, IgG2a) and cytokine (IL-1β, IFN-γ, IL-4) production was evaluated in an in vivo experiment. The results showed that all nano-adjuvant formulations had a strong influence in up-regulation of IFN-γ and IL-4 in parallel with high IgG1-IgG2a isotype antibody titers in mice. In particular, SWCNT-AL-PEI nano-adjuvant formulation generated a balanced Th1 and Th2 immune response with more biased toward Th1 response without exhibiting any inflammatory and toxic effects. Therefore this nano-adjuvant formulation could be used as an efficient prophylactic immune responses agent.

  12. Effects of different proportions of high carbohydrate intake on morbidity of hypertension of middle aged and elderly people in Zhang Fang village, Fang Shan District, Beijing%高碳水化合物摄入比例对北京市房山区张坊村中老年人高血压患病率的影响

    Institute of Scientific and Technical Information of China (English)

    洪忠新; 王佳; 武力; 顾中一; 毕研霞; 丁冰杰; 李伟; 张立红

    2012-01-01

    目的 探讨碳水化合物摄入比例与北京市房山区张坊村中老年人高血压患病率的关系.方法 随机抽取北京市房山区张坊村中老年村民150例,测量血压和人体指标,并对村民膳食摄入量进行调查,按纳入和排除标准入选132例作为研究对象.根据碳水化合物的摄入比例将村民分成两组:高碳水化合物摄入比例组(碳水化合物摄入比例占总能量比例大于或等于60%组),以下称高碳水化合物组(high carbohydrate group,HC组),和非高碳水化合物摄入比例组(碳水化合物摄入比例占总能量比例小于60%组),以下称非高碳水化合物组(none high carbohydrate group,NHC组),并对两组人群的有关指标进行统计分析.结果 该村中老年高血压患病率较高,达59.1%.HC组高血压患病率明显高于NHC组,以收缩压升高为显著;两组村民年龄、性别、吸烟和饮酒情况差异无统计学意义;两组村民人体测量指标差异无统计学意义;两组村民总能量、碳水化合物、钙、钠及钾等摄入量差异无统计学意义,但NHC组村民鱼肉类、干果类和油脂类摄入量显著高于HC组,按营养素换算后NHC组村民蛋白质和脂肪摄入量亦显著高于HC组.结论 碳水化合物摄入占总能量比例大于或等于60%时,高血压患病率明显增加,以收缩压升高为显著.这与蛋白质和脂肪的摄入量的减少有关,与年龄、吸烟、饮酒、人体测量指标及总能量、碳水化合物、钙、钠及钾等摄入量无关.%Objective To invesligale the relationship belween the proportion of carbohydrale inlake and the morbidily of hypertension in middle aged and elderly people in Zhang Fang village, Fang Shan Dislricl, Beijing. Methods Tolally 150 middle aged and elderly villagers were selecled randomly in Zhang Fang Village,Fang Shan Dislricl, Beijing. The btood pressure and paramelers of anlhropomelry were measured and the diel inlake was investigated. According to

  13. Gal-PEG-PEI/psiRNA肝靶向性纳米基因载体对人胎肝细胞系L-02细胞的转染效率检测%Transfection efficiency of galactosylated poly (ethylene glycol) -graft-polyethylenimine as hepatocyte-tar geting psiRNA carrier in human fetus liver cells L-02 cells

    Institute of Scientific and Technical Information of China (English)

    聂常富; 张彤; 陈规划; 韩风; 邱大鹏; 王云检; 庞春

    2011-01-01

    目的:检测半乳糖-聚乙二醇-聚乙烯亚胺/小干扰RNA质粒(Gal-PEG-PEI/psiRNA)肝靶向性纳米基因载体对人胎肝细胞系L-02细胞的转染效率.方法:合成Gal-PEG-PEI/psiRNA纳米复合物,采用纳米粒径仪测定复合物的粒径和zeta电位,用不同Gal-PEG-PEI氨基与siRNA磷酸基比例(N/P)的Gal-PEG-PEI/psiRNA纳米复合物转染L-02细胞,以Lipofectamine 2000、非靶向性载体PEG-PEI/psiRNA和裸siRNA转染为对照组,48 h后用流式细胞仪测定转染效率.转染前加入1 mg半乳糖观察其半乳糖竞争拮抗结果.结果:Gal-PEG-PEI/psiRNA纳米复合物的粒径随N/P增大而减小,N/P≥1/15时达最小粒径,约为80 nm.Gal-PEG-PEI/psiRNA纳米基因载体的转染率为(20.4±0.9)%,明显高于非肝靶向性载体PEG-PEI/psiRNA(P<0.01)及裸psiRNA (P <0.01),但低于Lipofectamine 2000(P<0.05);加入竞争性拮抗剂半乳糖后GaI-PEG-PEI/psiRNA纳米基因载体的转染率下降至(4.8±2.1)%,PEG-PEI/psiRNA介导的转染率则不受影响.结论:Gal-PEG-PEI/psiRNA纳米基因载体可明显提高人胎肝细胞系L-02细胞的转染效率,且具有良好的肝细胞靶向性.%Objective; To evaluate the transfection efficiency of galactosylated poly (ethylene glycol) -graft-polyethylenimine (Gal-PEG-PEI) as a nonviral gene carrier of hepatocyte-targeting plasmid siRNA (psiRNA ) in human fetus liver cells L-02 cells. Methods: The characteristics of the Gal-PEG-PEI/psiRNA nanoparticles were measured by dynamic light scattering. The transfection experiments were performed with the Gal-PEG-PEI/psiRNA using GFP as the reporter gene in L-02 cells and the transfection efficiency was evaluated by flow cytometry after 48 h. The competition assay was carried out to confirm the uptake of Gal-PEG-PEI /psiRNA by ASGP-R in L-02 cells by adding l mg galactose into the transfection media. Results: The results indicated that the particle sizes decreased with increasing charge ratios of Gal-PEG-PEI to psi

  14. Fang CO2 med Aminosyrer

    DEFF Research Database (Denmark)

    Lerche, Benedicte Mai

    2010-01-01

    Med såkaldte “carbon capture-teknikker” er det muligt at rense røgen fra kulfyrede kraftværker, således at den er næsten helt fri for drivhusgassen CO2. Kunsten er at gøre processen tilstrækkeligt billig. Et lovende fangstredskab i denne proces er aminosyrer.......Med såkaldte “carbon capture-teknikker” er det muligt at rense røgen fra kulfyrede kraftværker, således at den er næsten helt fri for drivhusgassen CO2. Kunsten er at gøre processen tilstrækkeligt billig. Et lovende fangstredskab i denne proces er aminosyrer....

  15. An investigation into the Jin xiao fang (Prescriptions of Instant Effect), a lost recipe book of the Tang Dynasty%关于唐代已佚方书《近效方》的考察

    Institute of Scientific and Technical Information of China (English)

    黄斌; 张银柱

    2013-01-01

    《外台秘要方》引用了现已佚唐代方书《近效方》的141首方剂.对其引文进行分析,可知《近效方》由唐人李谏议所编写,大约成书于724-733年之间.《外台秘要方》引用的141首方剂涉及到临床各科,其来源也比较复杂,既有医人,也有士人,但所有方剂均具有“效验”的特点.%Altogether 141 prescriptions in the Jin xiao fang (Prescriptions of Instant Effect),a lost recipe book of the Tang Dynasty are cited in the Wai tai mi yao (Medical Secrets of An Official).Based on the analysis of these citations,it can be sure that the Jin xiao fang was written by Li Jian-yi of the Tang Dynasty,and completed around 724 to 733.These cited prescriptions are related to the clinical subjects,with complicate origins,for example,physicians and scholars.It is characterized by its "xiao yan (efficacy)"

  16. Membrane Fouling in Cu2+ Removal Process of Ultrafiltration of PEI-Cu2+ Complex in Water%聚乙烯亚胺(PEI)络合-超滤处理水中Cu2+过程中的膜污染

    Institute of Scientific and Technical Information of China (English)

    赵倩

    2011-01-01

    该文依据膜流动电位及zeta电位的测定原理设计了一套试验装置,可对中空纤维膜的流动电位进行测定.通过该装置研究了聚偏氟乙烯(PVDF)中空纤维超滤膜的动电现象在聚乙烯亚胺(PEI)络合-超滤处理水中Cu2+过程中的作用及规律,以此分析膜污染发生的过程与机理,为如何减轻和清洗超滤膜污染提供了依据.%According to the theories of membrane streaming potential and zeta potential, an experimental device was designed to measure streaming potential of hollow fiber membranes. By this design, the process of uItrafiltration of PEI-Cu2+ complex in water, changes of removal rate of Cu2* and streaming potential of the membrane were examined. The electrokinetic phenomenon and mechanism of poly vinylidine fluoride (PVDF) hollow fiber membrane pollution were studied and data for the reduction and cleaning of the membrane contamination were given in this paper.

  17. Textual research on Chen Ye and his Jia cang jing yan fang (Family-presreved Empirical Recipes) of the Song Dynasty%宋代陈哗及《家藏经验方》考

    Institute of Scientific and Technical Information of China (English)

    张雪丹; 张如青; 陈德兴

    2014-01-01

    Chen Ye,an official of the Southern Song Dynasty,also known as Chen Rihua as his styled name,was born in Changle,Fuzhou in the reign of Shaoxing,and died during the reign of Duanping.He had been consecutively in the positions of Jiang shi lang (Court Gentleman for Ceremonial Service),Zhi zhou (Prefect) of Lingding,the Ti xing (Judicial Commissioner) of Guangdong,the Zong ling (Overseer-general) of Sichuan,Shah ding (Reviser),Shu lin and other positions in Tongzhou,Yuanzhou.His works included 1 volume of Gu ling xian sheng nian pu (Mr.Guling's Chronological Biography),1 volume of Tan xie (On Humor),1 volume of Shi hua (Poetry),8 volumes of Jin yuan li shu (Jin Yuan's Smart Technique),3 volumes of Yi jian zhi lei bian (Classified Compilation of Yijian's Annals),(Zeng guang) Suo s ui lu (Augmented Records of Trivial Matters),5 volumes of Jia cang jing yah fang (Family-preserved Empirical Recipes).He also compiled the 8-volume Yinjiang zhi (Yinjiang's Annals),published the 2-volume Jia cang ji yao fang (Collected Essential Recipes from Family Preservation),and other proses and poetry.Jia cangjing yah fang was a formulary compiled by Chen Ye,which was lost.Altogether 74 of its recipes were cited in Fu ren da quan liang fang (Complete Effective Prescriptions for Women's Diseases),Shou qin yang lao shu (A Book for Pursuing Seniors' Longevity and Healthcare),Pu ji fang (Prescriptions for Universal Relief) and Yong le da dian (Yongle Encyclopedia).%南宋官员陈哗,字日华,福州长乐人,约生于宋绍兴年间,卒于端平年间,历任将仕郎、临汀知州、广东提刑、四川总领、删定、书林等职,亦曾于通州、沅州等地任职,著有《古灵先生年谱》1卷、《谈谐》1卷、《诗话》1卷、《金渊利术》8卷、《夷坚志类编》3卷、《(增广)琐碎录》、《家藏经验方》5卷,主持编撰《鄞江志》8卷,刊行方导《家藏集要方》2卷,另有诗词散文等.《家藏经验方》为陈晔所编撰的一部方

  18. PEI改性介孔分子筛KIT-6对CO2吸附性能的研究%Study on CO2 Adsorption Properties of PEI-Modified Mesoporous Molecular Sieve KIT-6

    Institute of Scientific and Technical Information of China (English)

    张中正; 王慧; 魏伟; 孙予罕

    2014-01-01

    CO2 capture has gained great attention due to its accumulation in the atmosphere, which is widely agreed as the predominant cause of greenhouse effect. In order to find an efficient adsorbent with high CO2 adsorption capacity and separation selectivity, mesoporous silica KIT-6 was synthesized by hydrothermal method and its amine-functionalized by impregnating it with PEI (polyethylenimine). The CO2 adsorption performances of KIT-6 and its modified ones were evaluated by using a dynamic adsorption apparatus. The effects of different PEI-loading ratioes on the adsorption of CO2 were investigated by the changes of breakthrough time, adsorption capacity and separation coefficient. The results show that PEI-modified KIT-6 adsorbent could greatly enhance CO2 adsorption amount and the separation coefficient between CO2 and N2. The CO2 adsorption performance increases with PEI-loading and then decreases. When the PEI-loading ratio is 0.6, the adsorption amount of CO2 and the separation selectivity reach their maximum value of 2.09 mmol×g-1 and 30.56, respectively, which are 6.0 times and 7.2 times higher than that of KIT-6 without PEI loading. Moreover, the CO2 adsorption capacity of PEI-modifies KIT-6 increases with temperature. Besides, the saturated PEI-modified adsorbent can be fully regenerated at 378 K and the regenerated adsorbent shows fairly good stability in 20 cycles of adsorption/regeneration.%CO2的大量排放是造成温室效应的主要因素,对全球排放的CO2进行捕集非常重要。今采用浸渍法将PEI(聚乙烯亚胺)负载到KIT-6介孔分子筛孔道表面上,使孔的吸附作用和胺的吸收作用相结合,制备出吸附容量大和选择性高的CO2吸附剂,从穿透时间、吸附量、分离因子等方面研究了PEI负载率对CO2吸附性能的影响。结果表明:KIT-6介孔分子筛经PEI改性后对CO2的吸附量和选择性增强。随着PEI负载量的增大,吸附剂对CO2的吸附性能先增大

  19. Small angle neutron scattering (SANS and V-SANS) study of asphaltene aggregates in crude oil.

    Science.gov (United States)

    Headen, Thomas F; Boek, Edo S; Stellbrink, Jörg; Scheven, Ulrich M

    2009-01-06

    We report small angle neutron scattering (SANS) experiments on two crude oils. Analysis of the high-Q SANS region has probed the asphaltene aggregates in the nanometer length scale. We find that the radius of gyration decreases with increasing temperature. We show that SANS measurements on crude oils give similar aggregate sizes to those found from SANS measurements of asphaltenes redispersed in deuterated toluene. The combined use of SANS and V-SANS on crude oil samples has allowed the determination of the radius of gyration of large scale asphaltene aggregates of approximately 0.45 microm. This has been achieved by the fitting of Beaucage functions over two size regimes. Analysis of the fitted Beaucage functions at very low-Q has shown that the large scale aggregates are not simply made by aggregation of all the smaller nanoaggregates. Instead, they are two different aggregates coexisting.

  20. Vertical tectonic deformation associated with the San Andreas fault zone offshore of San Francisco, California

    Science.gov (United States)

    Ryan, H. F.; Parsons, T.; Sliter, R. W.

    2008-10-01

    A new fault map of the shelf offshore of San Francisco, California shows that faulting occurs as a distributed shear zone that involves many fault strands with the principal displacement taken up by the San Andreas fault and the eastern strand of the San Gregorio fault zone. Structures associated with the offshore faulting show compressive deformation near where the San Andreas fault goes offshore, but deformation becomes extensional several km to the north off of the Golden Gate. Our new fault map serves as the basis for a 3-D finite element model that shows that the block between the San Andreas and San Gregorio fault zone is subsiding at a long-term rate of about 0.2-0.3 mm/yr, with the maximum subsidence occurring northwest of the Golden Gate in the area of a mapped transtensional basin. Although the long-term rates of vertical displacement primarily show subsidence, the model of coseismic deformation associated with the 1906 San Francisco earthquake indicates that uplift on the order of 10-15 cm occurred in the block northeast of the San Andreas fault. Since 1906, 5-6 cm of regional subsidence has occurred in that block. One implication of our model is that the transfer of slip from the San Andreas fault to a fault 5 km to the east, the Golden Gate fault, is not required for the area offshore of San Francisco to be in extension. This has implications for both the deposition of thick Pliocene-Pleistocene sediments (the Merced Formation) observed east of the San Andreas fault, and the age of the Peninsula segment of the San Andreas fault.

  1. 75 FR 55270 - Safety Zone; NASSCO Launching of USNS Washington Chambers, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2010-09-10

    ... Chambers, San Diego Bay, San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the navigable waters of the San Diego Bay in... the Port (COTP) San Diego or his designated representative. DATES: This rule is effective from 9:15 a...

  2. Description of gravity cores from San Pablo Bay and Carquinez Strait, San Francisco Bay, California

    Science.gov (United States)

    Woodrow, Donald L.; John L. Chin,; Wong, Florence L.; Fregoso, Theresa; Jaffe, Bruce E.

    2017-06-27

    Seventy-two gravity cores were collected by the U.S. Geological Survey in 1990, 1991, and 2000 from San Pablo Bay and Carquinez Strait, California. The gravity cores collected within San Pablo Bay contain bioturbated laminated silts and sandy clays, whole and broken bivalve shells (mostly mussels), fossil tube structures, and fine-grained plant or wood fragments. Gravity cores from the channel wall of Carquinez Strait east of San Pablo Bay consist of sand and clay layers, whole and broken bivalve shells (less than in San Pablo Bay), trace fossil tubes, and minute fragments of plant material.

  3. San Luis Valley waterbird plan : Draft

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The goal of this plan is "to provide and protect a habitat base of sufficient quality and quantity to maintain healthy viable populations of waterbirds in the San...

  4. Historical methyl mercury in San Francisco Bay

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — San Francisco Bay, California is considered a mercury-impaired watershed. Elevated concentrations of mercury are found in water and sediment as well as fish and...

  5. Bathymetry--Offshore of San Francisco, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the bathymetry and shaded-relief maps of Offshore of San Francisco, California (raster data file is included in...

  6. Backscatter A [8101]--Offshore San Gregorio, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the acoustic-backscatter map (see sheet 3, SIM 3306) of the Offshore of San Gregorio map area, California. Backscatter data...

  7. Habitat--Offshore of San Francisco, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the habitat map of the seafloor of the Offshore of San Francisco map area, California. The vector data file is included in...

  8. San Antonio Bay 1986-1989

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The effect of salinity on utilization of shallow-water nursery habitats by aquatic fauna was assessed in San Antonio Bay, Texas. Overall, 272 samples were collected...

  9. Habitat--Offshore of San Francisco, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the habitat map of the seafloor of the Offshore of San Francisco map area, California. The vector data file is included in...

  10. Contours--Offshore of San Francisco, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the bathymetric contours for several seafloor maps of the Offshore of San Francisco map area, California. The vector data file...

  11. Backscatter B [7125]--Offshore San Gregorio, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the acoustic-backscatter map (see sheet 3, SIM 3306) of the Offshore of San Gregorio map area, California. Backscatter data...

  12. San Bernardino National Wildlife Refuge contaminant study

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The San Bernardino National Wildlife Refuge was established in 1982 for the protection and management of endangered desert fishes which are indigenous to the Rio...

  13. On Mr. Cai Yuan-pei's Sports Thought and its Inspirations%论蔡元培先生的体育思想及其启示

    Institute of Scientific and Technical Information of China (English)

    杜景强

    2013-01-01

    Mr. Cai Yuan-pei is a famous educator and practitioner in Chinese modern history of education, he presented a thought of military country education from the perspective of“resisting foreign imposition and saving motherland, strengthening military and enric-hing country”;advocated the idea of“sports first in full personality”, emphasizing that physical education plays a fundamental role in the comprehensive development education; focused the popularity of sports, specially thinking that citizens should develop lifelong physical exercise regularly;advocated actively to encourages and support women's equality to participate in sports. This paper considers that the application of Cai Yuan-pei's sports ideology will be helpful for sports workers to learn from previous useful thinking and elimi-nate negative factors that probably arise in current physical education.%蔡元培先生是中国近代教育史上著名的教育家和实践家,他站在“御侮救国,强兵富国”的立场提出了军国民体育思想;提倡“完全人格,首在体育”的体育教育主张强调体育在全面发展教育中,具有基础性的重要作用;在强调体育的普及的同时,还特别强调国民应该养成经常锻炼的终身体育思想。提倡积极鼓励和大力支持妇女平等的参加体育。将蔡元培的体育思想运用到当前体育教育中,有助于广大体育工作者吸取前人有益的思维成果,消除当前体育教育工作中可能出现的不利因素。

  14. An Archeological Survey of the San Diego River

    Science.gov (United States)

    1975-08-27

    Indian populations and to arable landa and water, Father Serra moved Mission San Diego de Aleala ------ to the site of Nipoguay, an Indian village located...NN ARCHAEOLOGICAL SRE OF THE ~i7 SAN DIEGORIE I If SAN DIEGO STATE UNIVERSITY FOUNDATION DEPARTMENT OF THE ARMY, CORPS OF ENGINEERS kSAN DIEGO STATE...the San Diego River Archeological Survey A. PERFORMING ORG.ý REPORT NUM§ER na 7. AUTHOR(s) 6. CONTRACT OR GRANT NUMBER(*) - I- PERFORMING ORGANIZATION

  15. SSC San Diego Strategic Plan. Revision 3

    Science.gov (United States)

    2001-11-01

    OMB control number. 1. REPORT DATE NOV 2001 2. REPORT TYPE 3. DATES COVERED - 4. TITLE AND SUBTITLE SSC San Diego Strategic Plan. Rev 3 5a...Business Improvement Group ( CBIG ), will be composed of the team members listed above, and will be responsible for monitoring the planning and...implementation of these objectives. The CBIG will charter sub-groups as necessary. Improve Corporate IT infrastructure Much of the SSC San Diego IT service

  16. Trouble Brewing in San Diego. Policy Brief

    Science.gov (United States)

    Buck, Stuart

    2010-01-01

    The city of San Diego will face enormous budgetary pressures from the growing deficits in public pensions, both at a state and local level. In this policy brief, the author estimates that San Diego faces total of $45.4 billion, including $7.95 billion for the county pension system, $5.4 billion for the city pension system, and an estimated $30.7…

  17. Abrupt along-strike change in tectonic style: San Andreas Fault zone, San Francisco Peninsula

    Science.gov (United States)

    Zoback, Mary Lou; Jachens, Robert C.; Olson, Jean A.

    1999-05-01

    Seismicity and high-resolution aeromagnetic data are used to define an abrupt change from compressional to extensional tectonism within a 10- to 15-km-wide zone along the San Andreas fault on the San Francisco Peninsula and offshore from the Golden Gate. This 100-km-long section of the San Andreas fault includes the hypocenter of the Mw = 7.8 1906 San Francisco earthquake as well as the highest level of persistent microseismicity along that ˜470-km-long rupture. We define two distinct zones of deformation along this stretch of the fault using well-constrained relocations of all post-1969 earthquakes based a joint one-dimensional velocity/hypocenter inversion and a redetermination of focal mechanisms. The southern zone is characterized by thrust- and reverse-faulting focal mechanisms with NE trending P axes that indicate "fault-normal" compression in 7- to 10-km-wide zones of deformation on both sides of the San Andreas fault. A 1- to 2-km-wide vertical zone beneath the surface trace of the San Andreas is characterized by its almost complete lack of seismicity. The compressional deformation is consistent with the young, high topography of the Santa Cruz Mountains/Coast Ranges as the San Andreas fault makes a broad restraining left bend (˜10°) through the southernmost peninsula. A zone of seismic quiescence ˜15 km long separates this compressional zone to the south from a zone of combined normal-faulting and strike-slip-faulting focal mechanisms (including a ML = 5.3 earthquake in 1957) on the northernmost peninsula and offshore on the Golden Gate platform. Both linear pseudogravity gradients, calculated from the aeromagnetic data, and seismic reflection data indicate that the San Andreas fault makes an abrupt ˜3-km right step less than 5 km offshore in this northern zone. A similar right-stepping (dilatational) geometry is also observed for the subparallel San Gregorio fault offshore. Persistent seismicity and extensional tectonism occur within the San Andreas

  18. 凉血通瘀中药治疗脑出血急性期168例疗效观察%Liangxue Tongyu Fang for treating acuter-phase cerebral hemorrhage in 168 cases

    Institute of Scientific and Technical Information of China (English)

    过伟峰; 王敬卿; 赵扬; 邵凤扬; 陈隐漪; 顾锡镇; 王永生; 路楷; 张兰坤; 吴勉华; 李国春; 周学平; 叶放; 袁园; 全亚萍; 陈顺中

    2012-01-01

    目的 评价凉血通瘀中药治疗脑出血急性期的临床疗效.方法 将337例患者随机分为治疗组168例和对照组169例,2组均采用西医内科常规治疗,治疗组同时服用凉血通瘀中药,疗程均为21 d.疗程结束后分别统计并比较2组综合疗效、中风病类诊断评分、脑出血吸收情况、脑水肿分级及格拉斯哥预后结果( GOS)评分.结果 治疗组总有效率88.0%,高于对照组的77.5%(P<0.05).2组治疗后中风病类诊断评分及GOS评分均较治疗前显著下降(P<0.01),且治疗组优于对照组(P <0.05或P<0.01).结论 结合凉血通瘀中药治疗脑出血急性期能显著提高临床疗效,改善预后.%Objective To review the curative effect of Liangxue Tongyu Fang on acute-phase cerebral hemorrhage. Methods All patients (n =337) were randomly divided into treatment group (n - 168) and control group (n = 169). The two groups were given routine therapies of Western internal medicine, and treatment group was given Liangxue Tongyu Fang at the same time. A therapeutic course was 21 days. The comprehensive curative effect, diagnostic scores of stroke, absorption of cerebral hemorrhage, haematoma grading and scores of Glasgow outcome score (GOS) were counted and compared in two groups. Results The total effective rate was 88. 0% in treatment group, which was higher than that (77. 5% ) in control group (P <0. 05) . The diagnostic scores of stroke and GOS decreased significantly in two groups after the treatment than before (P <0. 01) , which was more significant in treatment group than that in control group ( P < 0. 05 or P < 0. 01 ). Conclusion Liangxue Tongyu Fang can significantly improve the curative effect and outcomes in the treatment of acute cerebral hemorrhage.

  19. Changes of powder prescriptions in Qian jin yao fang (Essential Prescriptions Worth A Thousand Gold) made by Song people%简述宋人对《千金要方》散剂的改动

    Institute of Scientific and Technical Information of China (English)

    曾凤

    2008-01-01

    两种重要传本、在内容上多有不同,其中在方剂文献方面的差异特别值得重视.宋人对原书的散剂相关内容进行了诸多改动,包括论述散剂的章节与内容、药味计量单位、药味重量、散剂制法等,其所有的改动均无任何标示,书前的"凡例"亦无任何说明.从内容来看,由于增补了诸多散剂相关医学资料,宋校本较之新雕本显然更为丰富,书籍的章节编排更为明确合理.作为中医临床百科全书,宋校本更便于阅读与使用.但从传统的文献整理的角度来看,径改原书确为学者之大忌.%As the contents in the two circulated versions of Qian jin yao fang (Essential Prescriptions Worth A Thousand Gold),i.e Bei ko qian jin yao fang (Essential Prescriptions Worth a Thousand Gold for Emergencies) and Xin diao sun zhen ren qian jin fang (Newly Printed Sun's Prescriptions Worth A Thousand Gold) aye different greatly,especially on the literature of prescriptions.Song people made a lot of changes on the contents of powder prescriptions,including the related chapters and its contents,computation unit of materia medica,weights,and processing methods etc.without any remarks,nor notes in its preface.The contents in the Song version were more enriched than the newly carved version with the reasonable arrangement of its texts.As a clinical encyclopedia of traditional Chinese medicine,the Song version is more convenient for reading and using.However,it is a great taboo for the scholars to tamper an ancient work at will from bibliographic viewpoints.

  20. Clinical Observation on Treating Colorectal Cancer Combined FuZheng KangAiFang with FOLFOX Chemotherapy%扶正抗癌方结合FOLFOX化疗方案治疗大肠癌的临床观察

    Institute of Scientific and Technical Information of China (English)

    曾英

    2011-01-01

    Objective:To observe the therapeutic effect of treating colorectal cancer combined FuZheng KangAiFang with FOLFOX chemotherapy Method:40 colorcctal cancer patients were randomly allocated into two groups: the integrative medicine group and chemotherapy group.Patients in two groups were chemotherapy with FOLFOX programe,the integrative medicine group were given additional FuZheng KangAiFang. Two weeks treatment as one cycle, After 3 cycles, size of tumor,CEA marks,side effects,the change of symptoms,quality of life and CD4/CD8 were compared between two groups.Result:The efficiency rates in the integrative medicine group and chemotherapy group were 57. 14% and 33.33%Conclusion:In the treatment of colorectal cancer, the remedy of chemotherapy plus FuZheng KangAiFang could play down the CEA marks,down-regulate the side effects,regulate the symptom of TCM,improve the life quality, effectively meliorate the immunity state,had effects of synergistic and attenuation.%目的:观察扶正抗癌方结合FOLFOX化疗方案治疗大肠癌的临床疗效.方法:将40例大肠癌患者随机分为中西医结合组和单纯化疗组.2组均采用FOLFOX方案全身化疗,中西医结合组加用扶正抗癌方口服.3个周期后观察2组患者肿瘤大小、癌胚抗原(CEA)水平、化疗不良反应、症状改善、生活质量和CD4/CD8比值变化.结果:中西医结合组有效率为57.14%,单纯化疗组为33.33%.结论:扶正抗癌方结合FOLFOX化疗方案治疗大肠癌能够降低肿瘤标志物CEA水平、减少化疗副作用、改善患者中医证候、提高患者生活质量、调节免疫状态,即起到了减毒增效作用.

  1. Low-temperature growth and physical investigations of undoped and (In, Co) doped ZnO thin films sprayed on PEI flexible substrate

    Science.gov (United States)

    Ben Ameur, S.; Barhoumi, A.; Mimouni, R.; Amlouk, M.; Guermazi, H.

    2015-08-01

    ZnO thin films were deposited on polymer substrate Polyethyerimide (PEI) at 250 °C by spray pyrolysis technique. The effects of different doping elements (Co and In) on physical properties of ZnO thin films were investigated. Thin film characterizations were carried out using X-ray diffraction technique, UV-Vis-NIR spectroscopy, Photoluminescence (PL) spectroscopy and the contact angle measurement method. XRD measurement showed a successful growth of crystalline films on polymer substrate at low temperature by the spray pyrolysis process. XRD patterns revealed that all films consist of single ZnO phase and were well crystallized with preferential orientation towards (1 0 1) direction. Doping by cobalt has effective role in the enhancement of the crystalline quality, increases in the band gap according to Burstein Moss effect. Doping with indium leads rather to the decrease of both crystallinity and optical band gap energy value. Photoluminescence of the films showed UV emission (NBE) and visible emission related to defects. The contact angles were measured to study the effect of various doping elements on the hydrophobicity of the film depending on surface roughness. Results showed strong dependence on the doping element. In fact, doping with cobalt element increases the roughness of ZnO films and reinforces the surface from hydrophilic to hydrophobic (θ > 90°).

  2. "A different kind of beauty": scientific and architectural style in I.M. Pei's Mesa Laboratory and Louis Kahn's Salk Institute.

    Science.gov (United States)

    Leslie, Stuart W

    2008-01-01

    I.M. Pei's Mesa Laboratory for the National Center for Atmospheric Research in Boulder, Colorado, and Louis Kahn's Salk Institute in La Jolla, California, are rare examples of laboratories as celebrated for their architecture as for their scientific contributions. Completed in the mid-1960s, these signature buildings still express the scientific style of their founding directors, Walter Roberts and Jonas Salk. yet in commissioning their laboratories, Roberts and Salk had to work with architects as strong-willed as themselves. A close reading of the two laboratories reveals the ongoing negotiations and tensions in collaborations between visionary scientist and visionary architect. Moreover, Roberts and Salk also had to become architects of atmospheric and biomedical sciences. For laboratory architecture, however flexible in theory, necessarily stabilizes scientific practice, since a philosophy of research is embedded in the very structure of the building and persists far longer than the initial vision and mission that gave it life. Roberts and Salk's experiences suggest that even the most carefully designed laboratories must successfully adapt to new disciplinary configurations, funding opportunities, and research priorities, or risk becoming mere architectural icons.

  3. Suppression of tumor growth in lung cancer xenograft model mice by poly(sorbitol-co-PEI)-mediated delivery of osteopontin siRNA.

    Science.gov (United States)

    Cho, Won-Young; Hong, Seong-Ho; Singh, Bijay; Islam, Mohammad Ariful; Lee, Somin; Lee, Ah Young; Gankhuyag, Nomundelger; Kim, Ji-Eun; Yu, Kyeong-Nam; Kim, Kwang-Ho; Park, Young-Chan; Cho, Chong-Su; Cho, Myung-Haing

    2015-08-01

    Small interfering RNA (siRNA)-mediated gene silencing represents a promising strategy for treating diseases such as cancer; however, specific gene silencing requires an effective delivery system to overcome the instability and low transfection efficiency of siRNAs. To address this issue, a polysorbitol-based transporter (PSOT) was prepared by low molecular weight branched polyethylenimine (bPEI) crosslinked with sorbitol diacrylate (SDA). Osteopontin (OPN) gene, which is highly associated with non-small cell lung cancer (NSCLC) was targeted by siRNA therapy using siRNA targeting OPN (siOPN). Characterization study confirmed that PSOT formed compact complexes with siOPN and protected siOPN against degradation by RNase. PSOT/siOPN complexes demonstrated low cytotoxicity and enhanced transfection efficiency in vitro, suggesting that this carrier may be suitable for gene silencing. In the A549 and H460 lung cancer cell lines, PSOT/siOPN complexes demonstrated significant silencing efficiency at both RNA and protein levels. To study in vivo tumor growth suppression, two lung cancer cell-xenograft mouse models were prepared and PSOT/siOPN complexes were delivered into the mice through intravenous injection. The siOPN-treated groups demonstrated significantly reduced OPN expression at both the RNA and protein levels as well as suppression of tumor volume and weight. Taken together, siOPN delivery using PSOT may present an effective and novel therapeutic system for lung cancer treatment.

  4. Descenso de las propiedades mecánicas del pei reforzado con fibra de vidrio y carbono en ambientes químicos

    Directory of Open Access Journals (Sweden)

    Viña, J.

    2000-08-01

    Full Text Available This work shows how the mechanical properties, tensile and shear strength, are modified in different way if two different types of composite materials are immersed in toluen and sulfuric acid. The composites have a thermoplastic as matrix and one of them is reinforced with glass fiber and the other with carbon fiber. Also the important influence of the temperature has been tested in the case of the acid and specimens with holes have been tested to know their influence.

    El presente trabajo muestra la diferente influencia que sobre las propiedades mecánicas, resistencias a tracción y cortadura, tiene la inmersión en dos agentes químicos, tales como, tolueno y ácido sulfúrico de dos tipos diferentes de materiales compuestos, uno reforzado con fibra de vidrio y otro con fibra de carbono. En ambos casos la matriz empleada es un termoplástico de altas prestaciones denominado PEI. En el caso del ataque con el ácido se ha podido, asimismo, contrastar la importante influencia de la temperatura en la disminución de las resistencias y por último, también se han ensayado, a tracción, probetas que contenían agujeros pasantes para, en este caso, descartar que el aumento de la superficie de ataque aumentaba éste.

  5. Poly-cross-linked PEI through aromatically conjugated imine linkages as a new class of pH-responsive nucleic acids packing cationic polymers

    Directory of Open Access Journals (Sweden)

    Shun eChen

    2016-02-01

    Full Text Available Cationic polyimines polymerized through aromatically conjugated bis-imine linkages and intra-molecular cross-linking were found to be a new class of effective transfection materials for their flexibility in structural optimization, responsiveness to intracellular environment, the ability to facilitate endosome escape and cytosol release of the nucleic acids, as well as self-metabolism. When three phthalaldehydes of different substitution positions were used to polymerize highly branched low molecular weight polyethylenimine (PEI-1.8K, the product through ortho-phthalimines (named PPOP showed significantly higher transfection activity than its two tere- and iso-analogues (named PPTP and PPIP. Physicochemical characterization confirmed the similarity of three polyimines in pH-responded degradability, buffer capacity, as well as the size and Zeta potential of the polyplexes formed from the polymers. A mechanistic speculation may be that the ortho-positioned bis-imine linkage of PPOP may only lead to the straight trans-configuration due to steric hindrance, resulting in larger loops of intra-polymer cross-linking and more flexible backbone.

  6. 适用于高温高矿化度条件的聚乙烯亚胺冻胶堵水剂%Water shutoff agent of PEI gel applicable to high temperature and high salinity reservoirs

    Institute of Scientific and Technical Information of China (English)

    吴运强; 毕岩滨; 纪萍; 汪进; 贾寒; 周洪涛

    2015-01-01

    A gel type shutoff agent with anionic polyacrylamide as a main agent required for high temperature and high salinity formation water shutoff was developed using a crosslinking agent of water soluble polymer-polyethylenimine (PEI), and protective agent was added to slow down hydrolysis of the main agent under high temperature. The impact of main agent mass fraction, main agent molecular weight, PEI mass fraction, PEI molecular weight, salinity and pH was experimentally investigated on gelling performance of PEI gel. Experimental results show that the relative molecular weights of main agent and PEI increase as the mass fraction of main agent and PEI increases while the gelation time is shortened. But under high mass fraction, excessive crosslinking is more likely to occur, causing premature gel dehydration. The gelation time is shortened with the increase of pH values, and becomes longer with the increase of salinity. At 110℃, for formation water with NaCl content of 50 000 mg / L, the gel shows high strength and good resistance to temperature and good blocking effectiveness, and remains hydrated for at least 120 days. The optimum formula for water shutoff system is 0.6% to 0.8% anionic polyacrylamide & 0.3% to 0.7% PEI & 0.8% to 1% high temperature protective agent.%针对高温高矿化度地层堵水需求,研制了一种耐高温高矿化度的以阴离子聚丙烯酰胺为主剂的冻胶型堵剂,交联剂采用水溶性聚合物聚乙烯亚胺(PEI),并加入保护剂减缓高温下主剂的水解。实验考察了主剂质量分数、主剂分子量、PEI质量分数、PEI分子量、矿化度和pH值等因素对PEI冻胶成胶性能的影响。实验结果表明:随着主剂和PEI相对分子质量增加,主剂和PEI质量分数的增加,成胶时间缩短,但交联剂质量分数过高易发生过交联现象,造成冻胶过早脱水;成胶时间随着pH值的增加而缩短,随矿化度的增加而变长。在110℃条

  7. Synthesis of SAN-PB-SAN triblock copolymers via a ''living'' copolymerization with macro-photoiniferters

    NARCIS (Netherlands)

    Kroeze, E; de Boer, B.; ten Brinke, G.; Hadziioannou, G

    1996-01-01

    A technique is described for the synthesis of poly((styrene-co-acrylonitrile)-block-butadiene-block-(styrene-co-acrylonitrile)) (SAN-PB-SAN) triblock copolymers through polybutadiene-based photo-iniferters. Dihydroxy- and dicarboxy-terminated polybutadienes were transformed into the chloro-terminate

  8. 76 FR 1386 - Safety Zone; Centennial of Naval Aviation Kickoff, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2011-01-10

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Centennial of Naval Aviation Kickoff, San... in support of the Centennial of Naval Aviation Kickoff. This temporary safety zone is necessary to... Purpose On February 12, 2010, the Centennial of Naval Aviation Kickoff will take place in San Diego Bay...

  9. 77 FR 59969 - Notice of Inventory Completion: San Francisco State University, Department of Anthropology, San...

    Science.gov (United States)

    2012-10-01

    ... Anthropology, San Francisco, CA; Correction AGENCY: National Park Service, Interior. ACTION: Notice; correction... Department of Anthropology). The human remains and associated funerary objects were removed from Marin County... San Francisco State University Department of Anthropology records. In the Federal Register (73...

  10. Una Visita al Viejo San Juan (A Visit to Old San Juan).

    Science.gov (United States)

    Cabello, Victor; And Others

    Written in Spanish, this black and white illustrated booklet provides a tour of Old San Juan, Puerto Rico's oldest and most historic city. Brief historical information is provided on the Perro de San Jeronimo, a statue of a barking dog found in front of the Castillo; Plaza de Colon, a small plaza dedicated to Christopher Columbus; the Catedral de…

  11. Una Visita al Viejo San Juan (A Visit to Old San Juan).

    Science.gov (United States)

    Cabello, Victor; And Others

    Written in Spanish, this black and white illustrated booklet provides a tour of Old San Juan, Puerto Rico's oldest and most historic city. Brief historical information is provided on the Perro de San Jeronimo, a statue of a barking dog found in front of the Castillo; Plaza de Colon, a small plaza dedicated to Christopher Columbus; the Catedral de…

  12. 33 CFR 165.754 - Safety Zone: San Juan Harbor, San Juan, PR.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety Zone: San Juan Harbor, San Juan, PR. 165.754 Section 165.754 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED ACCESS AREAS Specific Regulated Navigation Areas and...

  13. Self-assembly of various Au nanocrystals on functionalized water-stable PVA/PEI nanofibers: a highly efficient surface-enhanced Raman scattering substrates with high density of "hot" spots.

    Science.gov (United States)

    Zhu, Han; Du, MingLiang; Zhang, Ming; Wang, Pan; Bao, ShiYong; Zou, Meiling; Fu, YaQin; Yao, JuMing

    2014-04-15

    We have demonstrated a facile approach for the fabrication of flexible and reliable sulfydryl functionalized PVA/PEI nanofibers with excellent water stability for the self-assembly of Au nanocrystals, such as Au nanoparticles (AuNPs), Au nanoflowers (AuNFs) and Au nanorods (AuNRs), used as the highly efficient surface-enhanced Raman scattering (SERS) substrates for the detection of rhodamine B (RhB). Various methods were employed to cross-link the PVA nanofibers with better morphology and porous structures after immersing in water for desired times. Various SERS-active Au nanocrystals, such as AuNPs, AuNFs, and AuNRs have been successfully synthesized. After the grafting of MPTES on the cross-linked PVA/PEI nanofibers, the Au nanocrystals can easily be self-assembled on the surfaces of the nanofibers because of the strong interactions of the Au-S chemical bondings. The Au nanocrystals self-assembled throughout the PVA/PEI nanofibers used as SERS substrates all exhibit enhanced SERS signals of RhB compared with their individual nanocrystals. It is mainly due to the close interparticle distance, mutual orientation and high density of "hot" spots, that can strongly affect the overall optical response and the SERS enhancement. By changing the amounts of the self-assembled AuNFs on the nanofibers, we can control the density of the "hot" spots. With the increased amounts of the AuNFs throughout the nanofibers, the SERS substrates show enhanced Raman signals of the RhB, indicating that the increased density of "hot" spots can directly lead to the SERS enhancement. The AuNFs/(PVA/PEI) SERS substrates show good sensitivity, reliability and low detection limit (10(-9) M). The presented approach can be broadly applicable to the assembly of different types of plasmonic nanostructures and these novel materials with strong SERS enhancement can be applied in bioanalysis and biosensors.

  14. 78 FR 19103 - Safety Zone; Spanish Navy School Ship San Sebastian El Cano Escort; Bahia de San Juan; San Juan, PR

    Science.gov (United States)

    2013-03-29

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Spanish Navy School Ship San Sebastian El... during the transit of the Spanish Navy School Ship San Sebastian El Cano, a public vessel, and during... board the Spanish Navy School Ship San Sebastian El Cano. The inbound escort is scheduled to take...

  15. Effective of Hui Medicine AikangFang Synergism Chemotherapy to Lewis Lung Carcinoma Cell Ultrastructure%回药爱康方协同化疗对小鼠Lewis肺癌细胞超微结构的影响

    Institute of Scientific and Technical Information of China (English)

    马治国; 马科; 周丽萍; 边静; 周慧

    2012-01-01

    Objective Observed effective of Hui medicine AikangFang synergism chemotherapy to mice Lewis lung cancer cell changes by electron microscope. Methods We uesed to 55 C57 mice which were inoculated Lewis lung carcinoma cells after 24 h were randomly divided into model group, chemotherapy group, Hui medicine AikangFang; AikangFang low - dose and high - dose chemotherapy group. After 14d treatment, we observed the general situation of mice, completely stripping tumor tissue that the tumor, lung cells dissection, e-lectron microscope drawn, fabrication, observed in each group changes in the structure of tumor cells. Results Compared with model group, the rest of group mice general situation were good, high - dose plus chemotherapy group was best, the mice tumor weight compared with model group were statistically significant ( P < 0.01 ). Treatment groups of lung cancer cells have different degrees of morpho logical damage, high - dose chemotherapy group the most obvious cell damage, a large number of organelles necrosis, chromatin high degree of concentration. Conclusion Hui medicine AikangFang affects cancer cells , the high - dose chemotherapy can be effectively coordinated damage tumor cells.%目的 观察回药爱康方协同化疗对小鼠Lewis肺癌细胞超微结构的影响.方法 把55只C57小鼠接种Lewis肺癌细胞24h后随机分为模型组、化疗组、爱康方组、爱康方低剂量加化疗组、爱康方高剂量加化疗组.治疗14d后,观察小鼠一般情况,完整剥离肿瘤组织称瘤重,电镜取材、制作,观察各组肿瘤细胞结构的改变.结果 与模型组比较,其余各组小鼠一般情况较好,高剂量加化疗组最佳,小鼠瘤重较模型组轻,差异均有统计学意义(P<0.01);与模型组比较,其余各组的肺癌细胞形态都有不同程度的损伤,高剂量加化疗组细胞破坏最明显,出现大量细胞器坏死,染色质高度聚集.结论 回药爱康方高剂量协同化疗可有效损伤肿瘤细胞.

  16. Fissile Material Disposition Program: Deep Borehole Disposal Facility PEIS data input report for direct disposal. Direct disposal of plutonium metal/plutonium dioxide in compound metal canisters. Version 3.0

    Energy Technology Data Exchange (ETDEWEB)

    Wijesinghe, A.M.; Shaffer, R.J.

    1996-01-15

    The US Department of Energy (DOE) is examining options for disposing of excess weapons-usable nuclear materials [principally plutonium (Pu) and highly enriched uranium (HEU)] in a form or condition that is substantially and inherently more difficult to recover and reuse in weapons production. This report is the data input report for the Programmatic Environmental Impact Statement (PEIS). The PEIS examines the environmental, safety, and health impacts of implementing each disposition alternative on land use, facility operations, and site infrastructure; air quality and noise; water, geology, and soils; biotic, cultural, and paleontological resources; socioeconomics; human health; normal operations and facility accidents; waste management; and transportation. This data report is prepared to assist in estimating the environmental effects associated with the construction and operation of a Deep Borehole Disposal Facility, an alternative currently included in the PEIS. The facility projects under consideration are, not site specific. This report therefore concentrates on environmental, safety, and health impacts at a generic site appropriate for siting a Deep Borehole Disposal Facility.

  17. Fissile Material Disposition Program: Deep Borehole Disposal Facility PEIS data input report for direct disposal. Direct disposal of plutonium metal/plutonium dioxide in compound metal canisters. Version 3.0

    Energy Technology Data Exchange (ETDEWEB)

    Wijesinghe, A.M.; Shaffer, R.J.

    1996-01-15

    The US Department of Energy (DOE) is examining options for disposing of excess weapons-usable nuclear materials [principally plutonium (Pu) and highly enriched uranium (HEU)] in a form or condition that is substantially and inherently more difficult to recover and reuse in weapons production. This report is the data input report for the Programmatic Environmental Impact Statement (PEIS). The PEIS examines the environmental, safety, and health impacts of implementing each disposition alternative on land use, facility operations, and site infrastructure; air quality and noise; water, geology, and soils; biotic, cultural, and paleontological resources; socioeconomics; human health; normal operations and facility accidents; waste management; and transportation. This data report is prepared to assist in estimating the environmental effects associated with the construction and operation of a Deep Borehole Disposal Facility, an alternative currently included in the PEIS. The facility projects under consideration are, not site specific. This report therefore concentrates on environmental, safety, and health impacts at a generic site appropriate for siting a Deep Borehole Disposal Facility.

  18. Superparamagnetic Iron Oxide (Fe3O4 Nanoparticles Coated with PEG/PEI for Biomedical Applications: A Facile and Scalable Preparation Route Based on the Cathodic Electrochemical Deposition Method

    Directory of Open Access Journals (Sweden)

    Isa Karimzadeh

    2017-01-01

    Full Text Available Cathodic electrochemical deposition (CED is introduced as an efficient and effective method for synthesis and surface coating of superparamagnetic iron oxide nanoparticles (SPIONs. In this way, bare Fe3O4 nanoparticles were electrosynthesized through CED method from aqueous solution Fe3+ : Fe2+ chloride (molar ratio of 2 : 1. In the next step, the surface of NPs was coated with polyethyleneimine (PEI and polyethylene glycol (PEG during the CED procedure, and PEG/PEI coated SPIONs were obtained. The prepared NPs were evaluated by powder X-ray diffraction (XRD, Fourier transform infrared spectroscopy (FTIR, thermogravimetric analysis (TGA, dynamic light scattering (DLS, vibrating sample magnetometer (VSM, and field-emission scanning electron microscopy (FE-SEM. The pure magnetite phase and nanosize (about 15 nm of the prepared NPs were confirmed by XRD and FE-SEM. The presence of two coats (i.e., PEG and PEI on the surface of electrosynthesized NPs was proved via FTIR results. The percentage of polymer coat (37.5% on the NPs surface was provided by TGA analysis. The high magnetization value, negligible coercivity, and remanence measured by VSM indicate the superparamagnetic nature of both prepared NPs. The obtained results confirmed that the prepared Fe3O4 nanoparticles have suitable physicochemical and magnetic properties for biomedical applications.

  19. municipios Maracaibo y San Francisco

    Directory of Open Access Journals (Sweden)

    Rosa E. Ortiz

    2005-01-01

    Full Text Available El estudio de las Organizaciones No Gubernamentales estuvo dirigido a caracterizar la diversidad de grupos y asociaciones que bajo el termino genérico de ONG’s existen en los municipios Maracaibo y San Francisco, los más poblados y relevantes en la vida política, económica, social y cultural del estado Zulia-Venezuela. Se planteó una investigación exploratoria y descriptiva. El resultado permitió reconocer a Existe un crecimiento sostenido de las ONG en estos municipios: b los sectores con mayor concentración son el de salud; educación; deporte y recreación; arte y cultura. c Las instituciones que promueven más organizaciones son la iglesia y el Estado, lo que supone es este último caso una reducción de las funciones que le corresponden, además de la minimización de costos y conflictos laborales. d Ambivalencia en la orientación de su funcionamiento: Algunas ONG’s implementan prácticas próximas a la mercantilización de los servicios con lo cual legitiman estrategias del modelo económico neoliberal mientras otras contribuyen a fortalecer la democratización de los servicios públicos. Se concluye que el crecimiento y características de las ONGs en estos municipios estuvo vinculado en los noventa y comienzos de la década de 2000 por la aplicación de las políticas públicas neoliberales.

  20. 山田业广《千金要方读书记》初探%Preliminary study on Yamada's Qian jin yao fang du shu ji (Reading Notes of Essential Recipes Worth A Thousand Gold)

    Institute of Scientific and Technical Information of China (English)

    李俊德

    2008-01-01

    Yamada was a Japanese famous textology expert and bibliographer of Kampo medicine in the middle and late Edo period, one of his representative works is Qian jin yao fang du shu ji ( Reading Notes of Essential Recipes Worth A Thousand Gold), which was completed after he studied Qian jia yao fang (Essential Recipes Worth A Thousand Gold) , with the Song edition of Edo medical library as the master text it check against the Yuan edition, Zbengde edition, Wanli edition, Jiajing edition and handcopied genuine version. The above editions were the best contemporary version even until today, with high literature values. Yamada made a perfect texture research on Qian jin yaofang with familiar textology knowledge so as to cor-rect mistakes, elaborate annotations and judge common words. Unfortunately, there were seldom new ideas and several checked mistakes and wrong annotations.%山田业广是江户中末期日本著名的汉方医学考据学专家、文献学家,是其代表著作之一.山田业广以江户医学馆模刻宋版作为底本,取元刊本、正德本、万历本、嘉靖本、手抄真本等为校本,对进行研究并完成的撰写.以上几种版本是当时乃至现在所存之最善者,具有较高的文献价值.山田业广在中运用熟稔的考据学知识,对进行精良的考证,纠正讹误、阐发文义、判断俗字等方面为其所得;但新颖见解较少,并存在误校误训之处.

  1. The Fang Zhiming Spirit is Quality Teaching Resource for Cadre Education%方志敏精神是干部教育的优质教学资源

    Institute of Scientific and Technical Information of China (English)

    胡松; 吴寒斌; 高虹

    2012-01-01

    以方志敏同志为核心的赣东北党在长期的革命斗争和根据地建设实践中凝结而成的"方志敏精神",在当时发挥了重要的历史作用,并因其具有鲜明的优质教育教学资源的核心特征,具有符合现代教育教学价值的丰富内涵,成为在新的历史条件下对领导干部进行理想信念教育、道德情操教育、爱国主义教育、时代精神教育、党的宗旨教育以及廉政教育等的优质资源。%The Fang Zhiming Spirit distilled by the CPC organization in the northeast Jiangxi,as led by Comrade Fang Zhiming,during the long-term practice of revolutionary struggle and construction of revolutionary base,played an important historical role.It conforms to the core characteristics of quality teaching resources and has enriched connotation conforming to the value of contemporary education,becoming a quality resource for carrying out faith education,moral education,patriotism education,time spirit education,party tenet education,and anti-corruption education to Led leader cadres.

  2. San Pascual (2007) Año XLV, n. 344

    OpenAIRE

    2007-01-01

    Editorial. Coronación pontificia y declaración del patronato canónico para nuestra señora de Gracia. siguiendo los caminos de San Pascual, capítulo XIII Madrid II. Entrevista, Rosario García García presidenta de la asociación de hijas de María del Rosario.¡Seréis mis testigos (II)!. Donativos pro nueva bandera de San Pascual. San Pascual y la virgen de Gracia. Vida en el santuario. Los tesoros de San Pascual. Milagros de San Pascual VI, libra San Pascual a muchos del peligro del mar y de otro...

  3. San Diego Science Alliance Education Outreach Activities

    Science.gov (United States)

    Blue, Anne P.

    1996-11-01

    The General Atomics Science Education Outreach Activities as well as those of several other San Diego area institutions led to the formation in 1994 of the San Diego Science Alliance. The Science Alliance is a consortium of science-related industries, institutions of research and higher education, museums, medical health networks, and science competitions in support of K-12 science education. Some Alliance accomplishments include printing over 4000 resource catalogs for teachers, workshops presented by over 20 of their business members at the San Diego Science Education Conference, and hosting of 3 eight-week courses for teachers. The Alliance provides an important forum for interaction between schools and teachers and local industries and institutions. The Science Alliance maintains a World Wide Web Home Page at elvbf http://www.cerf.net/sd_science/. General Atomics' role in the San Diego Science Alliance will be presented.(Presented by Patricia S. Winter for the General Atomics Science Education Groups and San Diego Science Alliance.)

  4. Cacao use and the San Lorenzo Olmec.

    Science.gov (United States)

    Powis, Terry G; Cyphers, Ann; Gaikwad, Nilesh W; Grivetti, Louis; Cheong, Kong

    2011-05-24

    Mesoamerican peoples had a long history of cacao use--spanning more than 34 centuries--as confirmed by previous identification of cacao residues on archaeological pottery from Paso de la Amada on the Pacific Coast and the Olmec site of El Manatí on the Gulf Coast. Until now, comparable evidence from San Lorenzo, the premier Olmec capital, was lacking. The present study of theobromine residues confirms the continuous presence and use of cacao products at San Lorenzo between 1800 and 1000 BCE, and documents assorted vessels forms used in its preparation and consumption. One elite context reveals cacao use as part of a mortuary ritual for sacrificial victims, an event that occurred during the height of San Lorenzo's power.

  5. Salt Ponds, South San Francisco Bay

    Science.gov (United States)

    2002-01-01

    higher resolution 1000 pixel-wide image The red and green colors of the salt ponds in South San Francisco Bay are brilliant visual markers for astronauts. The STS-111 crew photographed the bay south of the San Mateo bridge in June, 2002. This photograph is timely because a large number of the salt ponds (more than 16,500 acres) that are owned by Cargill, Inc. will be sold in September for wetlands restoration-a restoration project second in size only to the Florida Everglades project. Rough boundaries of the areas to be restored are outlined on the image. Over the past century, more than 80% of San Francisco Bay's wetlands have been filled and developed or diked off for salt mining. San Francisco Bay has supported salt mining since 1854. Cargill has operated most of the bay's commercial salt ponds since 1978, and had already sold thousands of acres to the State of California and the Don Edwards National Wildlife Refuge. This new transaction will increase San Francisco Bay's existing tidal wetlands by 50%. The new wetlands, to be managed by the California Department of Fish and Game and the U.S. Fish and Wildlife Service, will join the Don Edwards National Wildlife Refuge, and provide valuable habitat for birds, fish and other wildlife. The wetlands will contribute to better water quality and flood control in the bay, and open up more coastline for public enjoyment. Additional information: Cargill Salt Ponds (PDF) Turning Salt Into Environmental Gold Salt Ponds on Way to Becoming Wetlands Historic Agreement Reached to Purchase San Francisco Bay Salt Ponds Astronaut photograph STS111-376-3 was provided by the Earth Sciences and Image Analysis Laboratory at Johnson Space Center. Additional images taken by astronauts and cosmonauts can be viewed at the NASA-JSC Gateway to Astronaut Photography of Earth

  6. Ectopic study of calcium phosphate cement seeded with pBMP-2 modified canine bMSCs mediated by a non-viral PEI derivative.

    Science.gov (United States)

    Lü, Kaige; Zeng, Deliang; Zhang, Wenjie; Xia, Lunguo; Xu, Ling; Jiang, Xinquan; Zhang, Fuqiang

    2012-02-01

    We have evaluated the ectopic new bone formation effects of CPC (calcium phosphate cement) seeded with pBMP-2 (plasmids containing bone morphogenetic protein-2 gene) transfected canine bMSCs (bone marrow stromal cells) mediated by a non-viral PEI (polyethylenimine) derivative (GenEscort™ II) in nude mice. Canine bMSCs were transfected with pBMP-2 or pEGFP (plasmids containing enhanced green fluorescent protein gene) mediated by GenEscort™ II in vitro, and the osteoblastic differentiation was explored by ALP (alkaline phosphatase) staining, ARS (alizarin red S) staining and RT-qPCR (real-time quantitative PCR) analysis. Ectopic bone formation effects of CPC/pBMP-2 transfected bMSCs were evaluated and compared with CPC/pEGFP transfected bMSCs or CPC/untransfected bMSCs through histological, histomorphological and immunohistochemical analysis 8 and 12 weeks post-operation in nude mice. Transfection efficiency was up ∼35% as demonstrated by EGFP (enhanced green fluorescent protein) expression. ALP and ARS staining were stronger with pBMP-2 gene transfection, and mRNA expression of BMP-2 (bone morphogenetic protein-2), Col 1 (collagen 1) and OCN (osteocalcin) in pBMP-2 group was significantly up-regulated at 6 and 9 days. Significantly higher NBV (new bone volume) was achieved in pBMP-2 group than in the control groups at 8 and 12 weeks (P<0.05). In addition, immunohistochemical analysis indicated higher OCN expression in pBMP-2 group (P<0.01). We conclude that CPC seeded with pBMP-2 transfected bMSCs mediated by GenEscort™ II could enhance ectopic new bone formation in nude mice, suggesting that GenEscort™ II mediated pBMP-2 gene transfer is an effective non-viral method and CPC is a suitable scaffold for gene enhanced bone tissue engineering.

  7. Acute toxicity, bioaccumulation and effects of dietary transfer of silver from brine shrimp exposed to PVP/PEI-coated silver nanoparticles to zebrafish.

    Science.gov (United States)

    Lacave, José María; Fanjul, Álvaro; Bilbao, Eider; Gutierrez, Nerea; Barrio, Irantzu; Arostegui, Inmaculada; Cajaraville, Miren P; Orbea, Amaia

    2017-09-01

    The extensive use and release to the aquatic environment of silver nanoparticles (NPs) could lead to their incorporation into the food web. Brine shrimp larvae of 24h showed low sensitivity to the exposure to PVP/PEI-coated Ag NPs (5nm), with EC50 values at 24h of 19.63mgAgL(-1), but they significantly accumulated silver after 24h of exposure to 100μgL(-1) of Ag NPs. Thus, to assess bioaccumulation and effects of silver transferred by the diet in zebrafish, brine shrimp larvae were exposed to 100ngL(-1) of Ag NPs as an environmentally relevant concentration or to 100μgL(-1) as a potentially effective concentration and used to feed zebrafish for 21days. Autometallography revealed a dose- and time-dependent metal accumulation in the intestine and in the liver of zebrafish. Three-day feeding with brine shrimps exposed to 100ngL(-1) of Ag NPs was enough to impair fish health as reflected by the significant reduction of lysosomal membrane stability and the presence of vacuolization and necrosis in the liver. However, dietary exposure to 100μgL(-1) of Ag NPs for 3days did not significantly alter gene transcription levels, neither in the liver nor in the intestine. After 21days, biological processes such as lipid transport and localization, cellular response to chemical stimulus and response to xenobiotic stimulus were significantly altered in the liver. Overall, these results indicate an effective dietary transfer of silver and point out to liver as the main target organ for Ag NP toxicity in zebrafish after dietary exposure. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Evaluación del comportamiento mecánico de compuestos PEI – fibra de vidrio ante solicitaciones térmicas

    Directory of Open Access Journals (Sweden)

    Chacón, L.

    2004-04-01

    Full Text Available The mechanical behavior of a composite with thermoplastic matrix and glass fiber reinforcement has been studied. It has been tested to constant stresses of a value equivalent to tensile strength divided by three to 50, 100 and 150 ºC, during periods of time between 250 and 3000 minutes. A decrement of the tensile strength and deformation can be observed in the three temperatures. Also a group of specimens have been exposed to accelerated aging in an environmental chamber to 70 ºC and 95% RH, during 30 days; and after they have been tested. In that case a decrement of the strength and deformation after 250 minutes has been observed, but in larger time of exposure these properties suffered an increment.

    Se ha estudiado el comportamiento mecánico de un compuesto fabricado con un termoplástico PEI (polieterimida reforzado con tejido de fibra de vidrio, frente a ensayos en los que se mantienen tensiones iguales a un tercio de su resistencia a tracción a 50, 100 y 150 ºC, durante periodos comprendidos entre 250 y 3000 minutos. En los resultados se observa una disminución de la resistencia y de la deformación en las tres temperaturas, con un mantenimiento de sus valores hasta las ensayadas a 3000 minutos. Además fueron expuestas un grupo de probetas del material, a envejecimiento acelerado en cámara higrotérmica a 70 ºC y a un 95% de H.R., durante 30 días; para luego someterlas a los ensayos antes descritos. Para esas probetas se obtuvieron pequeñas fluctuaciones de la resistencia y deformación con el tiempo, sin observarse una influencia significativa de la temperatura a la que se hicieron los ensayos termomecánicos.

  9. A Case for Historic Joint Rupture of the San Andreas and San Jacinto Faults

    Science.gov (United States)

    Lozos, J.

    2015-12-01

    The ~M7.5 southern California earthquake of 8 December 1812 ruptured the San Andreas Fault from Cajon Pass to at least as far north as Pallet Creek (Biasi et al., 2002). The 1812 rupture has also been identified in trenches at Burro Flats to the south (Yule and Howland, 2001). However, the lack of a record of 1812 at Plunge Creek, between Cajon Pass and Burro Flats (McGill et al., 2002), complicates the interpretation of this event as a straightforward San Andreas rupture. Paleoseismic records of a large early 19th century rupture on the northern San Jacinto Fault (Onderdonk et al., 2013; Kendrick and Fumal, 2005) allow for alternate interpretations of the 1812 earthquake. I use dynamic rupture modeling on the San Andreas-San Jacinto junction to determine which rupture behaviors produce slip patterns consistent with observations of the 1812 event. My models implement realistic fault geometry, a realistic velocity structure, and stress orientations based on seismicity literature. Under these simple assumptions, joint rupture of the two faults is the most common behavior. My modeling rules out a San Andreas-only rupture that is consistent with the data from the 1812 earthquake, and also shows that single fault events are unable to match the average slip per event for either fault. The choice of nucleation point affects the details of rupture directivity and slip distribution, but not the first order result that multi-fault rupture is the preferred behavior. While it cannot be definitively said that joint San Andreas-San Jacinto rupture occurred in 1812, these results are consistent with paleoseismic and historic data. This has implications for the possibility of future multi-fault rupture within the San Andreas system, as well as for interpretation of other paleoseismic events in regions of complex fault interactions.

  10. A Glorious Century of Art Education: San Francisco's Art Institute

    Science.gov (United States)

    Dobbs, Stephen Mark

    1976-01-01

    Author described the life and times of the San Francisco Art Institute and reviewed the forces that made San Francisco a city of more than ordinary awareness of the arts in its civic and civil existence. (Editor/RK)

  11. Species Observations (poly) - San Diego County [ds648

    Data.gov (United States)

    California Department of Resources — Created in 2009, the SanBIOS database serves as a single repository of species observations collected by various departments within the County of San Diego's Land...

  12. Mammal Track Counts - San Diego County, 2010 [ds709

    Data.gov (United States)

    California Department of Resources — The San Diego Tracking Team (SDTT) is a non-profit organization dedicated to promoting the preservation of wildlife habitat in San Diego County through citizen-based...

  13. Coastal Cactus Wren, San Diego Co. - 2009 [ds702

    Data.gov (United States)

    California Department of Resources — The San Diego Multiple Species Conservation program (MSCP) was developed for the conservation of plants and animals in the southeast portion of San Diego County....

  14. Coastal Cactus Wren, San Diego Co. - 2011 [ds708

    Data.gov (United States)

    California Department of Resources — The San Diego Multiple Species Conservation program (MSCP) was developed for the conservation of plants and animals in the southeast portion of San Diego County....

  15. Mammal Track Counts - San Diego County [ds442

    Data.gov (United States)

    California Department of Resources — The San Diego Tracking Team (SDTT) is a non-profit organization dedicated to promoting the preservation of wildlife habitat in San Diego County through citizen-based...

  16. San Marino-China Friendship Association 20 Years Old

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    <正>San Marino, with a population of less than 30,000, is one of the smallest countries in Europe. In such a small country the San Marino-China Friendship Association (SMCFA) was set up and has exerted

  17. Las fortalezas de San Lázaro

    Directory of Open Access Journals (Sweden)

    Enrique Naranjo Martínez

    1961-11-01

    Full Text Available Generalmente en Cartagena, al hablar de las fortalezas del cerro de San Lázaro, las llaman en conjunto el Castillo de San Felipe de Barajas, pero en esto nos parece que hay un error. El castillo propiamente dicho se construyó en 1657, según la inscripción que en placa de mármol, ostentaba El Caballero, desde donde la sonora voz de la campana daba los alertas a la plaza fuerte en los días de la colonia.

  18. Cacao use and the San Lorenzo Olmec

    OpenAIRE

    Powis, Terry G.; Cyphers, Ann; Gaikwad, Nilesh W.; Grivetti, Louis; Cheong, Kong

    2011-01-01

    Mesoamerican peoples had a long history of cacao use—spanning more than 34 centuries—as confirmed by previous identification of cacao residues on archaeological pottery from Paso de la Amada on the Pacific Coast and the Olmec site of El Manatí on the Gulf Coast. Until now, comparable evidence from San Lorenzo, the premier Olmec capital, was lacking. The present study of theobromine residues confirms the continuous presence and use of cacao products at San Lorenzo between 1800 and 1000 BCE, an...

  19. Southern San Francisco Bay Colonial Nesting Bird Census 1995-1996: Don Edwards San Francisco Bay National Wildlife Refuge Lands

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the 1995-1996 field season of the San Francisco Bay Bird Observatory (SFBBO) Colonial Waterbird Monitoring Study on the Don Edwards San...

  20. 33 CFR 110.210 - San Diego Harbor, CA.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false San Diego Harbor, CA. 110.210... ANCHORAGE REGULATIONS Anchorage Grounds § 110.210 San Diego Harbor, CA. (a) The anchorage grounds. (1... Commander, Naval Base, San Diego, CA. The administration of these anchorages is exercised by the...

  1. 78 FR 48646 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2013-08-09

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... at the Supervisor's Office of the Pike & San Isabel National Forests, Cimarron and Comanche...

  2. 75 FR 51749 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2010-08-23

    ... No: 2010-20802] DEPARTMENT OF AGRICULTURE Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Pike & San Isabel Resource... at the Supervisor's Office of the Pike & San Isabel National Forests, Cimarron and Comanche...

  3. 76 FR 30903 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2011-05-27

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... of the Pike & San Isabel National Forests, Cimarron and Comanche National Grasslands (PSICC) at...

  4. 75 FR 65609 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2010-10-26

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... Supervior's Office of the Pike & San Isabel National Forests, Cimarron and Comanche National...

  5. 76 FR 2331 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2011-01-13

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... of the Pike & San Isabel National Forests, Cimarron and Comanche National Grasslands (PSICC) at...

  6. 77 FR 50459 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2012-08-21

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... will be held at the Supervisor's Office of the Pike & San Isabel National Forests, Cimarron...

  7. 76 FR 27304 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2011-05-11

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... Pike & San Isabel National Forests, Cimarron and Comanche National Grasslands (PSICC) at 2840...

  8. 75 FR 78675 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2010-12-16

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... of the Pike & San Isabel National Forests, Cimarron and Comanche National Grasslands (PSICC) at...

  9. 76 FR 9540 - Pike & San Isabel Resource Advisory Committee

    Science.gov (United States)

    2011-02-18

    ... Forest Service Pike & San Isabel Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Pike & San Isabel Resource Advisory Committee will meet in Pueblo, Colorado... of the Pike & San Isabel National Forests, Cimarron and Comanche National Grasslands (PSICC) at...

  10. 76 FR 12692 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2011-03-08

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... comments should be sent to Attn: San Juan National Forest RAC, 15 Burnett Court, Durango, CO 81301...

  11. 76 FR 40876 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2011-07-12

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... Sonoran Meeting Rooms. Written comments should be sent to Attn: San Juan National Forest RAC, 15 Burnett...

  12. 75 FR 48306 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2010-08-10

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... comments should be sent to Attn: San Juan National Forest RAC, 15 Burnett Court, Durango, CO 81301...

  13. 75 FR 71179 - Environmental Impact Statement: San Diego County, CA

    Science.gov (United States)

    2010-11-22

    ... Federal Highway Administration Environmental Impact Statement: San Diego County, CA AGENCY: Federal... Environmental Impact Statement (EIS) will be prepared ] for a proposed highway project in San Diego County... Community Center, 2258 Island Avenue, San Diego, California 92102. FOR FURTHER INFORMATION CONTACT: Kevin...

  14. San Francisco Bay Long Term Management Strategy for Dredging

    Science.gov (United States)

    The San Francisco Bay Long Term Management Strategy (LTMS) is a cooperative effort to develop a new approach to dredging and dredged material disposal in the San Francisco Bay area. The LTMS serves as the Regional Dredging Team for the San Francisco area.

  15. Heidegger y el cristianismo de San Pablo y San Agustín

    Directory of Open Access Journals (Sweden)

    Francisco de Lara

    2007-01-01

    Full Text Available Este texto intenta mostrar el sentido de la interpretación de San Pablo y San Agustín que Heidegger lleva a cabo en sus primeros cursos de Friburgo. En concreto, se pretende apuntar al motivo por el que el joven Heidegger recupera aspectos del cristianismo para su proyecto filosófico y cuáles son los elementos concretos que las Epístolas de San Pablo y las Confesiones de San Agustín le aportan. De esta forma, se apreciará, entre otras cosas, la importancia concedida por Heidegger a la acentuación del mundo propio (Selbstwelt y de la temporalidad que es característica de la experiencia cristiana del vivir

  16. San Juan Islands National Wildlife Refuge : San Juan Wilderness : Wilderness management plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document is a plan regarding management of the San Juan Wilderness. After introducing the area, it analyzes current management practices against current public...

  17. Gravity cores from San Pablo Bay and Carquinez Strait, San Francisco Bay, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data release contains information on gravity cores that were collected by the U.S. Geological Survey in the area of San Pablo Bay and Carquinez Strait,...

  18. San Jose, California: Solar in Action (Brochure)

    Energy Technology Data Exchange (ETDEWEB)

    2011-10-01

    This brochure provides an overview of the challenges and successes of San Jose, CA, a 2008 Solar America City awardee, on the path toward becoming a solar-powered community. Accomplishments, case studies, key lessons learned, and local resource information are given.

  19. San Diego, California: Solar in Action (Brochure)

    Energy Technology Data Exchange (ETDEWEB)

    2011-10-01

    This brochure provides an overview of the challenges and successes of San Diego, CA, a 2007 Solar America City awardee, on the path toward becoming a solar-powered community. Accomplishments, case studies, key lessons learned, and local resource information are given.

  20. Habitat--Offshore of San Gregorio, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the habitat map of the seafloor (see sheet 7, SIM 3306) of the Offshore of San Gregorio map area, California. The vector data...

  1. San Francisco's New Zoo's Connections for Conservation.

    Science.gov (United States)

    Routman, Emily

    2001-01-01

    Provides information on a redevelopment project at the San Francisco Zoo known as the New Zoo. The explicit goal of the project is to inspire a sense of caring and appreciation for wildlife that is the foundation of a conservation ethic. (DDR)

  2. SANS observations on weakly flocculated dispersions

    DEFF Research Database (Denmark)

    Mischenko, N.; Ourieva, G.; Mortensen, K.;

    1997-01-01

    Structural changes occurring in colloidal dispersions of poly-(methyl metacrylate) (PMMA) particles, sterically stabilized with poly-(12-hydroxystearic acid) (PHSA), while varying the solvent quality, temperature and shear rate, are investigated by small-angle neutron scattering (SANS). For a mod...

  3. San Diego Zoo:Success in Breeding

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Giant pandas have become very popular in U.S.zoos. One in particular, the San Diego Zoo, has been extremely successful at making the pandas feel at home and getting them to breed. In 1999, it became home tothe first surviving panda cub born in the United States.

  4. Habitat--Offshore of San Gregorio, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the habitat map of the seafloor (see sheet 7, SIM 3306) of the Offshore of San Gregorio map area, California. The vector data...

  5. SANS analysis of aqueous ionic perfluoropolyether micelles

    CERN Document Server

    Gambi, C M C; Chittofrati, A; Pieri, R; Baglioni, P; Teixeira, J

    2002-01-01

    Preliminary SANS results of ionic chlorine terminated perfluoropolyether micelles in water are given. The experimental spectra have been analyzed by a two-shell ellipsoidal model for the micellar form factor and a screened Coulombic plus hard-sphere repulsion potential for the structure factor. (orig.)

  6. San Antonio, Texas: Solar in Action (Brochure)

    Energy Technology Data Exchange (ETDEWEB)

    2011-10-01

    This brochure provides an overview of the challenges and successes of San Antonio, TX, a 2008 Solar America City awardee, on the path toward becoming a solar-powered community. Accomplishments, case studies, key lessons learned, and local resource information are given.

  7. Contours--Offshore of San Gregorio, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the bathymetric contours for several seafloor maps (see sheets 1, 2, 3, 7, 10, SIM 3306) of the Offshore of San Gregorio map...

  8. Bathymetry--Offshore San Gregorio, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the bathymetry and shaded-relief maps (see sheet 1 and 2, SIM 3306) of the Offshore of San Gregorio map area, California....

  9. Nathaniel Hawthorne Elementary School: San Antonio, Texas.

    Science.gov (United States)

    American Educator, 1997

    1997-01-01

    Discusses the successful use of Core Knowledge Curriculum in one inner-city elementary school in San Antonio (Texas) that had previously reflected low student achievement, inconsistent attendance, and student behavioral problems. Improvements in these conditions as revealed through teacher observations are highlighted. (GR)

  10. 1986 San Salvador, El Salvador Images

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — At least 1,000 people killed, 10,000 injured, 200,000 homeless and severe damage in the San Salvador area. About 50 fatalities were the result of landslides in the...

  11. 77 FR 20379 - San Diego Gas &

    Science.gov (United States)

    2012-04-04

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission San Diego Gas & Electric Company v. Sellers of Energy and Ancillary Services Into Markets Operated by the California Independent System Operator Corporation and the California...

  12. 75 FR 8106 - Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara, and San Mateo...

    Science.gov (United States)

    2010-02-23

    ... Fish and Wildlife Service Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara... located in Alameda, Santa Clara, and San Mateo Counties of California. We provide this notice in... in Alameda, Santa Clara, and San Mateo Counties of California, consists of several non...

  13. 75 FR 8804 - Safety Zone; NASSCO Launching of USNS Charles Drew, San Diego Bay, San Diego, CA.

    Science.gov (United States)

    2010-02-26

    ... Diego Bay, San Diego, CA. AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the navigable waters of the San Diego Bay in support of... Captain of the Port (COTP) San Diego or his designated representative. DATES: This rule is effective from...

  14. 77 FR 48532 - Notice of Intent To Repatriate Cultural Items: San Diego State University, San Diego, CA

    Science.gov (United States)

    2012-08-14

    ... National Park Service Notice of Intent To Repatriate Cultural Items: San Diego State University, San Diego, CA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The San Diego State University... Diego State University Archaeology Collections Management Program. DATES: Representatives of any Indian...

  15. 75 FR 17329 - Safety Zone; Big Bay Fourth of July Fireworks, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2010-04-06

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Big Bay Fourth of July Fireworks, San Diego Bay, San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Notice of proposed rulemaking. SUMMARY: The Coast Guard proposes establishing a temporary safety zone on the navigable waters of the San Diego Bay in...

  16. 76 FR 70480 - Otay River Estuary Restoration Project, South San Diego Bay Unit of the San Diego Bay National...

    Science.gov (United States)

    2011-11-14

    ... Fish and Wildlife Service Otay River Estuary Restoration Project, South San Diego Bay Unit of the San Diego Bay National Wildlife Refuge, California; Environmental Impact Statement AGENCY: Fish and Wildlife... the San Diego Bay National Wildlife Refuge. This notice advises the public that we intend to gather...

  17. 78 FR 48044 - Safety Zone; San Diego International Airport Terminal Two West Grand Opening Fireworks; San Diego...

    Science.gov (United States)

    2013-08-07

    ... [Docket No. USCG-2013-0637] RIN 1625-AA00 Safety Zone; San Diego International Airport Terminal Two West Grand Opening Fireworks; San Diego, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone on the navigable waters of the San Diego Bay in support...

  18. San Pascual (2013) Año L, n. 366

    OpenAIRE

    Pérez, María Dolores, O.S.C. (Directora)

    2013-01-01

    Editorial. Actualidad: Su Santidad el Papa Benedicto XVI y su renuncia. Peregrinos de la Fe con San Pascual Baylón. Efemérides Pascualinas: 15 de febrero de 1946. Se inaugura en el punte del Mar de Valencia el casilicio de San Pascual Baylón, obra del escultor José Pascual Ortells. Otras efemérides. San Pascual en la cerámica: El Retablo cerámico de San Pascual, Burriana. San Pascual y los Papas: Papa Benedicto XV. Año de la Fe: Exposición de crucifijos en el museo "Pouet del Sant" del Monast...

  19. San Juan Uchucuanicu: évolution historique

    Directory of Open Access Journals (Sweden)

    1975-01-01

    Full Text Available La communauté de San Juan est reconnue depuis 1939. Une première partie concerne l’organisation de la reducción de San Juan vers le milieu du XVIe siècle. Le poids fiscal s’exerce durement sur le village et la crise est générale dans toute la vallée du Chancay au XVIIe. siècle. La christianisation des habitants est définitive au milieu de ce même siècle. C’est vers la fin du XVIIe siècle et durant tout le XVIIIe que se multiplient les conflits entre San Juan et les villages voisins liés aux terrains de pâture et à la possession de l’eau. La deuxième partie du travail concerne les rapports de la communauté de San Juan avec le Pérou contemporain : contrainte fiscale toujours très lourde durant la fin de l’époque coloniale, exactions des militaires juste avant l’indépendance. La période républicaine voit toujours les conflits avec les villages voisins mais aussi la naissance de familles qui cherchent à retirer le maximum de la communauté. Les terres sont divisées et attribuées : la détérioration de l’organisation communale traditionnelle est manifeste. L4es conflits se multiplient entre petits propriétaires, mais aussi avec les haciendas voisines : c’est l’apparition d’une véritable lutte de classes. La situation actuelle est incertaine, le poids de l’économie marchande se développe avec l’exode des jeunes. Que sera la communauté San Juan à la fin de ce siècle? La comunidad de San Juan está reconocida desde 1939. La primera parte concierne a la organización de la 'reducción' de San Juan hacia mediados del siglo XVI. El peso fiscal se ejerce duramente sobre el pueblo y en el siglo XVII la crisis es general en todo el valle de Chancay. Hacia mediados del mismo siglo la cristianización de los habitantes es definitiva. Es hacia fines del siglo XVII y durante todo el siglo XVIII que se multiplican los conflictos entre San Juan y los pueblos vecinos, los que están relacionados con los terrenos de

  20. 方氏头皮针改善颈型颈椎病颈痛症状的临床研究%Clinical Study of Fang's Scalp Acupuncture for Relieving Neck pain Induced by Cervical Type of Cervical Spondylosis

    Institute of Scientific and Technical Information of China (English)

    邓特伟; 邓丽丽; 周达君

    2013-01-01

    [目的]观察方氏头针治疗颈型颈椎病颈痛的临床疗效.[方法]选取70例颈型颈椎病患者,随机分为治疗组与对照组各35例.治疗组采用方氏头针治疗,对照组采用传统针刺治疗.使用NPQ颈痛量表分别于治疗前、治疗1周后和疗程完毕时对2组疗效进行评价.[结果]治疗1周后及治疗结束后,2组患者的NPQ量表得分均显著降低,与治疗前比较差异均有统计学意义(P< 0.05或P<0.01),说明2组对颈型颈椎病颈痛皆有显著疗效.治疗1周后及治疗结束后2组间比较,差异均无统计学意义(P>0.05),表明2组在改善颈型颈椎病颈痛方面疗效相当.[结论]方氏头针与传统针刺治疗颈型颈椎病颈痛皆有较好的临床疗效;跟传统针灸相比,方氏头皮针更无痛、安全、精准、便利,对治疗骨关节病有独特优势.%Objective To observe the therapeutic effect of Fang's scalp acupuncture for relieving neck pain induced by cervical type of cervical spondylosis.Methods Seventy neck pain patients induced by cervical type of cervical spondylosis were equally divided into two groups.The treatment group was given Fang's scalp acupuncture,and the control group was given conventional acupuncture.The therapeutic effect was evaluated with Northwick Park Neck Pain Questionnaire (NPQ) scoring before treatment,one week after treatment and at the end of treatment.Results One week after treatment and at the end of treatment,NPQ scores were decreased in both groups (P < 0.05 or P < 0.01 compared with those before treatment),but the difference of NPQ scores were insignificant between the two groups (P>0.05).Conclusion Fang's scalp acupuncture has similar effect on relieving neck pain of cervical type of cervical spondylosis to conventional acupuncture,and has the advantages of painlessness,safety,accuracy and convenience.

  1. Prevention of mannitol renal damage by enema of Tongfu Xiezhou Fang in 22 cases%通腑泻浊方灌肠预防甘露醇肾损害22例临床观察

    Institute of Scientific and Technical Information of China (English)

    陈可为; 白慧梅; 郭家奎; 张津玮

    2012-01-01

    Object To investigate the effect of enema of Tongfu Xiezhou Fang in prevention of mannitol renal damage during treatment in the patients with severe cerebral infarction without reducing the dose of mannital in order to keep the curative effect. Methods The patients (n =48) with severe cerebral infarction (Glasgow Coma Score≤8) were randomly divided into treatment group ( n = 22) and control group ( n = 26). Mannitol was used conventionally as main dehydrating agent, and treatment group was given retention enema of Tongfu Xiezhou Fang for 30 minutes (once a day) at the same time of applying mannitol, and control group was not given enema. The levels of serum cystatin C and serum creatinine (SCr), blood urea nitrogen (BUN) and 24-hour urine output were detected in two groups before treatment and on the 5 th day of treatment. Results There were 2 cases with mannitol renal damage in treatment group (9.1%). There was one dead case due to cerebral edema and 10 cases with mannitol renal damage in control group (38. 5% ). The difference between two groups had statistical significance after statistical comparison ( P <0.05). The comparison in indexes of kidney function showed that the results were better in treatment group than those in control group ( P < 0. 05 ). Conclusion The retention enema of Tongfu Xiezhou Fang has reliable curative effect of preventing mannitol renal damage in the patients with severe cerebral infarction.%目的 探讨治疗重症脑梗死患者在不减低甘露醇剂量以保持疗效的前提下,利用通腑泻浊方灌肠预防甘露醇肾损害的疗效.方法 将48例重症脑梗死患者(格拉斯哥昏迷评分≤8分)随机分为治疗组22例和对照组26例,常规应用甘露醇作为主要脱水剂,治疗组在应用甘露醇的同时加以中药保留灌肠30 min,1日1次;对照组用甘露醇时不予以灌肠.监测2组患者治疗前、治疗第5天血清胱抑素C、血肌酐、尿素氮、24 h尿量等.结果 治

  2. Clinical Observation of Modified Baoshen Fang NO.2 on Patients with Diabetic Nephropathy%改良保肾方Ⅱ号治疗糖尿病肾病临床观察

    Institute of Scientific and Technical Information of China (English)

    段昱方; 蔡朕; 赵文景; 张胜容

    2011-01-01

    Objective: To study the clinical effects of Modified Baoshen fang NO.2 on patients with Diabetic Nephropathy. Methods: The 79 patients with Diabetic Nephropathy were divided into 2 groups by random, and both of the two groups were treated by diet and Glycemic control. In the control of blood pressure and blood lipids, treatment group was also treated by Modified Baoshen fang NO.2. Both of the two groups were treated by three months. TCM syndrome score, urinary protein excretion, glomerular filtration rate, glycated hemoglobin and blood lipids were observed before and after the treatment between two groups.Results: The total effective rate of treatment group was 87.50%, in the control group, the total effective rate was 61.54%, and the difference between the two groups was statistically significant ( P<O.05 ) .The TCM syndrome score and the urinary protein excretion were decreased compared with before treatment in the two groups both, but the treatment group was significantly better than the control group (P<0.05).The glomerular filtration rate before and after treatment had no significant difference (P>0.05 ) in the two groups. After treatment, glycosylated hemoglobin, cholesterol and triglycerides were better than those before treatment (P<O.05), but there was no significant difference between groups (P>O.05). Conclusions:The Modified Baoshen fang NO.2. can significantly reduce urinary protein in patients with diabetic kidney disease, improving clinical symptoms, and it has a significant effect in treating diabetic nephropathy.%目的:观察改良保肾方Ⅱ号对糖尿病肾病患者的临床疗效.方法:将79例2型糖尿病肾病患者分为治疗组和对照组,两组病人均予基础治疗即:控制饮食、血糖、血压、血脂.在此基础上,治疗组加用改良保肾方Ⅱ号中药汤剂,两组疗程均为3个月.观察两组治疗前后中医证候评分、尿蛋白定量、肾小球滤过率、糖化血红蛋白及血脂等指标

  3. Influence of Qing Jin Zhu Yu Fang iontophoresis on the respiratory function of COPD patients%清金逐瘀方离子导入对COPD患者呼吸功能的影响

    Institute of Scientific and Technical Information of China (English)

    郭义娟; 赵晓霞; 秦玉玲; 黄立强

    2014-01-01

    Objective:To observe the influence of Qing Jin Zhu Yu Fang iontophoresis on the respiratory function of patients with chronic obstructive pulmonary disease(COPD).Methods:120 cases of COPD patients were selected from February 2011 to February 2013.According to random number table method,the patients were divided into the experimental group and the control group with 60 cases in each.The control group was given conventional western medicine treatment,and the experimental group was given Qing Jin Zhu Yu Fang iontophoresis on the basis of conventional western medicine treatment.After treatment for two weeks, the lung function of patients were compared,such as forced expiratory vital capacity(FVC),forced expiratory volume in 1 second(FEV1),FEV1/predicted value(FEV1%),blood gas analysis index(arterial blood oxygen partial pressure PaO2,carbon dioxide pressure PaCO2).Results:After treatment for two weeks,the lung functions of two groups were all significantly improved.FVC,FEV1, FEV1% of the experimental group improved more significantly than those of the control group,and the difference was statistically significant(P<0.05).Conclusion:On the basis of conventional western medicine treatment,adding Qing Jin Zhu Yu Fang iontophoresis can significantly improve lung function in COPD patients,reduce inflammation,and obtain apparent curative effect.%目的:观察清金逐瘀方离子导入对慢性阻塞性肺疾病(COPD)患者呼吸功能的影响。方法:2011年2月-2013年2月收治COPD患者120例,按随机数字表法分为试验组和对照组,每组60例。对照组给予常规西医治疗,试验组在常规西医治疗的基础上予以清金逐瘀方离子导入,治疗2周后对比两组肺功能,即用力呼气肺活量(FVC)、1 s用力呼气容积(FEV1)、FEV1/预计值(FEV1%),血气分析指标(动脉血氧分压 PaO2、二氧化碳分压 PaCO2)。结果:治疗2周后,两组患者肺功能均有显著改善,其中试验组 FVC、FEV1、FEV1%

  4. 归逍方治疗肾虚肝郁型多囊卵巢综合征临床研究%Clinical Study of Guixiao Fang on Polycystic Ovary Syndrome Patients with Kidney-Asthenia and Liver Depression Syndrome

    Institute of Scientific and Technical Information of China (English)

    杨正望; 赵娜; 全春梅

    2016-01-01

    目的:观察归逍方治疗多囊卵巢综合征肾虚肝郁证的临床疗效并探讨其作用机制。方法将60例多囊卵巢综合征患者随机分为治疗组和对照组各30例。治疗组予归逍方口服治疗,对照组予口服炔雌醇环丙孕酮片治疗,疗程3个月。观察比较两组总疗效及治疗前后促黄体生成素(LH)、卵泡刺激素(FSH)、睾酮(T)、体质量指数(BMI)、F-G评分、痤疮评分、卵巢体积、中医证候积分变化。结果治疗组总有效率为87.6%,对照组为63.3%,治疗组疗效优于对照组(P<0.05);治疗组在降低BMI、LH、中医证候积分及缩小卵巢体积方面疗效优于对照组(P<0.05);两组在降低T、LH/FSH 及改善患者多毛、痤疮方面疗效相当(P>0.05)。结论归逍方治疗肾虚肝郁型多囊卵巢综合征临床疗效确切,其作用机制可能与改善LH/FSH比值、降低T水平相关。%Objective To observe the clinical efficacy of Guixiao Fang on polycystic ovary syndrome (PCOS) patients with kidney-asthenia liver depression syndrome, and investigate the mechanism. Methods The sixty PCOS patients with kidney liver depression syndrome were randomly divided into the treatment group and the control group, 30 cases in each group. Patients in the treatment group were treated by Guixiao Fang, and the patients in the control group were given progesterone tablets of the estradiol. Results The total effective rate in treatment group is 87.6%, the control group is 63.3%, the treatment group is superior to the control group (P0.05). Conclusion Guixiao Fang shows obvious clinical effect in treating PCOS patients with kidney liver depression syndrome, its mechanism may be related with the improving LH/FSH and reducing the T level.

  5. 李培旭治疗慢性肾功能衰竭经验%Li Pei-xu's Experience of Treating Chronic Renal Failure

    Institute of Scientific and Technical Information of China (English)

    袁小飞; 许辉

    2016-01-01

    慢性肾功衰竭病因复杂,病机多变,病情加重的因素较多,容易受外邪、饮食失宜、劳倦、情志失调等的影响,另外因肾脏本身排出毒素和代谢废物的功能下降,也会导致病情加重而出现恶心呕吐、厌食、头痛等症状.李培旭根据多年的临床经验发现在慢性肾功能衰竭的不同的阶段,一定时期内疾病的病理变化相对稳定,只要辨证准确,分清主次,通过补益脾肾、通利六腑、疏利三焦、清热祛实、补气化瘀,配合化毒、解毒、排毒、攻毒等方法,在慢性肾功能衰竭的早期阶段及早干预,可使病变的肾功能得到逆转,或者病情的发展得到控制,防止病情快速向尿毒症发展.%Chronic renal function failure has complex etiology,and changeable pathogenesis and many factors which lead to disease aggravation such as exogenous factors,improper diet,fatigue,emotional disorders,etc.Besides,the kidney function of ejecting toxin and metabolic waste decline may also lead to worsening illness and symptoms such as nausea and vomiting,anorexia,headache,etc.According to many years of clinical experience,Li Pei-xu has found that the pathological changes of chronic renal failure are relatively stable in the different stages or during a given period of time,so as long as finding the accurate syndrome differentiation,distinguishing between primary and secondary,nourishing the spleen and kidney,unblocking six fu-organs,unblocking the three energizers,clearing away heat and removing sthenia,tonifying qi and removing blood stasis,chronic renal failure is given early intervention,the pathological changes of renal function can be reversed,or the development of the disease can be controlled and prevented from changing into uremia quickly.

  6. Brief Explanation of"Shi"of Bone in Qi Xiao Liang Fang(《奇效良方》Prescriptions of Wonderful Efficacy)%《奇效良方》骨之“势”浅解

    Institute of Scientific and Technical Information of China (English)

    郭玉晶; 陈居伟; 刘更生

    2012-01-01

    《奇效良方·正骨兼金镞门》是古代伤科文献中的重要资料,其所记载的人身“三百六十五骨节”中包括对于骨内物质“势、液、髓”的论述.这一论述在现存古代文献记载中较为少见,但它也清楚的表明古人对于人体构造是有过系统研究的,且观察比较仔细.文章对《奇效良方·正骨兼金镞门》所载“势、液、髓”中“势”进行了研究,通过“势”字的含义演变结合现代人体解剖中骨骼构造的研究进行系统分析,认为“势、液、髓”中“势”所指代相当于现代骨骼构造中骨小梁及部分骨密质结构.综上,文章意在引起中医学界对于中医学人体解剖生理的重视.%Volume 56 of Qi Xiao Liang Fang () Prescriptions of Wonderful Efficacy) ( ) is the essential material among the ancient literatures of traumatology. It records "three hundred and sixty - five joints"of the human body and discusses"Shi,Ye,Sui" ,the substances of bone. The statement is seldom seen in the present existing ancient documents, but it clearly indicates the systematic study of the human body structure and the intensive observation by the ancients. This paper focuses on the study on the understanding of" Shi" recorded in" Shi, Ye, Sui "of the Volume 56 of Qi Xiao Liang Fang (Prescriptions of Wonderful Efficacy) (). A systematic analysis was conducted through the study on the evolution on the meaning of" Shi" in combination with the skeleton structure in modern human body anatomy. It was viewed that" Shi"recorded in" Shi, Ye, Sui" implied the bone trabecula and a part of compact bone structure. In summary,the paper aims to draw the attention of the field of traditional Chinese medicine to the human anatomy and physiology of traditional Chinese medicine.

  7. 《鲁谼方氏族谱》的历史社会学解读%The Interpretation of Luhong Fang Family Genealogy from the Perspective of Historical Sociology

    Institute of Scientific and Technical Information of China (English)

    方金友

    2013-01-01

    近年来,随着我国经济社会的快速发展,民间续修族谱活动逐渐兴起,人们更多地给予族谱以正面的新认识。人们从族谱记载家族世系等情况中,可以了解姓氏源流、家族兴替、人口迁徙、族习门风乃至社会变故等历史,赋予族谱一定的历史价值与社会意义。《鲁谼方氏族谱》由桐城派古文家方东树撰修,具有较高的历史文献价值,折射出社会变迁与文化传承。受学术风气与交叉学科的影响,人们解读族谱的方式有所变化,族谱学研究的视野不断扩展,从历史社会学的角度解读族谱亦是一种尝试。%In recent years, with the rapid economic and social development of China, the activities of editing genealogy are gradually on the rise, which makes more and more people take new positive understanding of it. From the family lineage recorded in genealogy, people can get to know the family history, such as origin of the family name, rise and fall of the family, population migration, customs and moral standing, as well as social changes, which give genealogy certain historical value and social significance. The Luhong Fang Family Genealogy, which was written by Fang Dongshu,the Tongcheng school prose master, reflects social changes and cultural inheritance, and embodies high historical and cultural value. Under the influence of academic atmosphere and interdisciplinary, the horizon of genealogy research is expanding, which changes the way of people’s understanding of genealogy. To interpret genealogy from the perspective of historical sociology is also an attempt.

  8. 丹瓜方对转基因糖尿病小鼠心脏损伤的作用%Study of Dan Gua-Fang in Preventention and Treatment of Heart Injury of Genetically Induced Diabetic Mice

    Institute of Scientific and Technical Information of China (English)

    黄苏萍; 陈燕; 林妙娴; 颜群芳; 王思梅; 叶梦捷

    2014-01-01

    探讨丹瓜方对转基因糖尿病小鼠心脏组织的影响。32只A poE-/-小鼠数字随机等分为模型组、丹瓜方组、联合用药组(丹瓜方+吡格列酮)、吡格列酮组。喂以普通饲料,各组分别给药12周。小鼠处死,取心脏、匀浆,免疫组化测心脏血清反应因子(SR F)含量;解剖冠状动脉,光镜观察比较。结果:与模型组比较,联合用药组与丹瓜方组SR F阳性率降低有显著性差异(P<0.01);形态学观察,丹瓜方组与联合用药组冠状动脉内膜大部分无增厚。结果表明:丹瓜方对糖尿病小鼠心脏具有保护作用。%To observe the effect of D an Gua-Fang on the heart of genetically induced diabetic mice. T hirty-tw o A poE-/-diabetic m ice w ere random ly divided into the m odel group, D an-gua prescription group, the com bined treatm ent group (Dan-gua prescription+ pioglitazone) and pioglitazone group. E ach group w as given m edicine respectively, and fed w ith norm al diet for 12 w eeks. T he heart hom ogenates production w as used to m easure related indicators. T he expression of SR F in heart hom ogenates w as detected by im m unohistochem ical. T he C oronary w as observed under light m icroscopy. C om pared w ith the m odel group, there w ere significant difference of SR F positive rate betw een com bination group and D an-gua prescription group (P<0.01). T he m orphology of D an-gua prescription group and the com bination group w as significantly im proved: m ost of the tunica intim a of the coronary artery cannot be found thickening. C om pared w ith the control group, Pioglitazone group's effect is w eaker,butbetterthan thatofthe untreated group.D an G ua-Fang hasprotective effecton the heartofdiabetic m ice.

  9. High-rise buildings PeiDianJian within gas fire-extinguishing systems design change are discussed%高层建筑内变配电间气体灭火系统设计探讨

    Institute of Scientific and Technical Information of China (English)

    林孔学

    2011-01-01

    文章对高层建筑内变配电间常用气体灭火系统设计进行了深入分析,提出了目前设计存在的问题及注意事项,并结合工程实例进行了阐述。%This paper PeiDianJian commonly used in high-rise buildings change gas fire-extinguishing systems design are analysed,put forward the current design problems and precautions,and combined with engineering example is discussed in this paper.

  10. 松郁安神方对REM睡眠剥夺大鼠认知功能及神经递质的影响%Effects of Song-Yu-An-Shen-Fang on Learning and Memory Capability and Levels of neurotransmitter in REM Sleep Deprivation Rats

    Institute of Scientific and Technical Information of China (English)

    曾雪爱; 黄俊山; 王雅丽

    2012-01-01

    Objective: To observe the effects of the compound Song-Yu-An-Shen-Fang on learning and memory capability and levels of monamine-neurotransmitter in REM sleep deprivation rats. Methods: The rats were randomly divided into normal group, environment control group, REM sleep deprivation group, Song-Yu-An-Shen-Fang treatment group and alprazolam treatment group. Morris water maze was used to measure Learning and memory performance. The levels of NE, DA and 5-HT were determined by HPLC-ECD. Results; Song-Yu-An-Shen-Fang improved the learning and memory capability of REM sleep deprivation rats and regulated the levels of NE, DA and 5-HT in plasma. Conclusion: Song-Yu-An-Shen-Fang can significantly improve the learning and memory capability in REM sleep deprivation rats, the underlying mechanism may be related to regulate the levels of some neurotransmitters.%目的:研究松郁安神方对REM睡眠剥夺大鼠认知功能及血浆中神经递质的影响.方法:药物治疗5d后水迷宫实验观察大鼠的学习记忆,用高效液相-电化学检测NE、DA、5-HT的含量.结果:中药治疗后大鼠的学习记忆能力较模型组明显改善,NE含量明显降低,DA、5-HT含量明显升高.结论:松郁安神方不仅可改善REM睡眠剥夺大鼠的学习记忆能力,而且可调节大鼠睡眠相关物质NE、DA、5-HT的含量,其学习记忆能力的改变可能与某些神经递质的改变有关.

  11. San Cristobal Galapagos wind power project

    Energy Technology Data Exchange (ETDEWEB)

    Tolan, J. [Sgurr Energy, Glasgow (United Kingdom)

    2009-07-01

    The San Cristobal Galapagos wind power project was described. With its unique endemic flora and fauna, the Galapagos Islands were declared a world heritage site and marine reserve. The San Cristobal wind project was initiated in 1999 to reduce the environmental impacts of energy use on the island, and has been operational since 2007. Three 800 kW wind turbines have been installed in order to reduce 52 per cent of the island's diesel generation. The project's high penetration wind-diesel hybrid system included 300 kW diesel generators, a 13.2 kV utility distribution system, and six 300 kW wind turbines. The project is located outside of Galapagos Petrel flight paths and nesting areas. Turbines from a factory in Spain were used. The wind turbine foundation was constructed from concrete sand and stone mined on the island. Photographs of the installation process were included. tabs., figs.

  12. San Telmo, backpackers y otras globalizaciones

    Directory of Open Access Journals (Sweden)

    Fernando Firmo

    2015-12-01

    Full Text Available Este artículo pretende contribuir al debate sobre otras formas de globalización  presentando una etnografía realizada en el barrio de San Telmo sobre mochileros que combinan en sus experiencias viaje y trabajo. Su objetivo es viajar al mismo tiempo que sacan provecho de esto para conseguir el capital necesario que les permita continuar en movimiento alrededor del globo. En este texto quiero hablar sobre estos auténticos actores de la globalización popular que ponen el foco en procesos y agentes alternativos no hegemónicos y que en este caso desarrollan su actividad en el contexto de la experiencia mochilera en San Telmo, siendo mi intención enriquecer las reflexiones sobre la globalización desde abajo.

  13. SAFOD Penetrates the San Andreas Fault

    Directory of Open Access Journals (Sweden)

    Mark D. Zoback

    2006-03-01

    Full Text Available SAFOD, the San Andreas Fault Observatory at Depth (Fig. 1, completed an important milestone in July 2005 by drilling through the San Andreas Fault at seismogenic depth. SAFOD is one of three major components of EarthScope, a U.S. National Science Foundation (NSF initiative being conducted in collaboration with the U.S. Geological Survey (USGS. The International Continental Scientific DrillingProgram (ICDP provides engineering and technical support for the project as well as online access to project data and information (http://www.icdp-online.de/sites/sanandreas/news/news1.html. In 2002, the ICDP, the NSF, and the USGS provided funding for a pilot hole project at the SAFOD site. Twenty scientifi c papers summarizing the results of the pilot hole project as well as pre-SAFOD site characterization studies were published in Geophysical Research Letters (Vol.31, Nos. 12 and 15, 2004.

  14. Discovery Along the San Andreas Fault: Relocating Photographs From the 1906 Earthquake in San Francisco and San Mateo Counties

    Science.gov (United States)

    Grove, K.; Prentice, C.; Polly, J.; Yuen, C.; Wu, K.; Zhong, S.; Lopez, J.

    2005-12-01

    April of 2006 will mark the 100-year anniversary of the great 1906 San Francisco earthquake. This earthquake was important not only because of its human tragedy (thousands of dead or homeless people), but also because of its scientific significance. The 8.3 magnitude earthquake ruptured 430 km of the northern San Andreas fault (SAF) and lasted nearly one minute. Investigations after the earthquake led to discoveries that were the beginning of modern earthquake theories and measuring instruments. This was also one of the first large-scale natural disasters to be photographed. Our research group, which is part of the National Science Foundation funded SF-ROCKS program, acquired photographs that were taken shortly after the earthquake in downtown San Francisco and along the SAF in San Mateo County. The SAF photos are part of a Geographical Information System (GIS) database being published on a U.S. Geological Survey web site. The goal of our project was to improve estimates of photograph locations and to compare the landscape features that were visible after the earthquake with the landscape that we see today. We used the GIS database to find initial photo locations, and we then used a high-precision Global Positioning System (GPS) to measure the geographic coordinates of the locations once we matched our view to what we saw in a photo. Where possible, we used a digital camera to retake photos from the same position, to show the difference in the landscape 100 years later. The 1906 photos show fault zone features such as ground rupture, sag ponds, shutter ridges, and offset fences. Changes to the landscape since 1906 have included erosion and grading of the land, building of houses and other structures, and more tree cover compared to previous grassland vegetation. Our project is part of 1906 Earthquake Centennial activities; it is contributing to the photo archive that helps scientists and engineers who study earthquakes and their effects. It will also help the

  15. 新安医家方开的摩腹运气功法与养生思想%Abdomen-rubbing qigong exercise and health preservation concept of Fang Kai, a Xin’an medical physician

    Institute of Scientific and Technical Information of China (English)

    刘洋; 胡玲; 来丹丹; 段文秀; 许静; 吴子建; 李坛明

    2016-01-01

    摩腹运气功法为清代新安医家方开首创,是新安医学养生著作中的重要组成部分。它是一种按摩与导引相结合,经络理论与藏象理论相结合的特色鲜明的养生保健疗法。摩腹运气功法操作简单,安全无副作用,具有顾护脾胃,补气益精,调和营卫、疏通经络、宣畅气机等养生保健作用,为推广与普及摩腹运气功法,对其进行总结。%The abdomen-rubbing qigong exercise was first developed by Fang Kai, a Xin’an medical practitioner. It’s an essential part of Xin’an health preservation works. This health preservation method integrates massage with Daoyin and integrates meridian theory with Zangxiang theory. It’s safe, simple and has no side effects. Practice of this qigong exercise can protect the spleen and stomach, tonify qi and essence, harmonize Ying-Nutrients and Wei-Defense, unblock meridians and circulate qi. This paper aims to summarize and promote this exercise.

  16. Metabolite identification of seven active components of Huan-Nao-Yi-Cong-Fang in rat plasma using high-performance liquid chromatography combined with hybrid ion trap/time-of-flight mass spectrometry.

    Science.gov (United States)

    Wang, Minchao; Lu, Yanzhen; Liu, Jiangang; Li, Hao; Wei, Yun

    2016-02-01

    Huan-Nao-Yi-Cong-Fang (HNYCF) is a potential prescription in treating Alzheimer's disease. Seven constituents [ferulic acid (FA), 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (THSG), berberine hydrochloride (BHCl), emodin, ginsenoside Rg1 (Rg1), ginsenoside Re (Re) and ginsenoside Rb1 (Rb1)] have been used as quality chemical markers of HNYCF owing to their biological significance and high contents in crude plant materials. This study explored the metabolites of the seven bioactive components in rat plasma to give useful data for further study of the action mechanism of HNYCF. LC/MS-IT-TOF was used to simultaneously characterize the metabolites of the seven components. Using the combination of MetID Solution 1.0 software and accurate mass measurements, the metabolites of HNYCF were reliably characterized. Their structures were elucidated based on the accurate MS(2) spectra and comparisons of their changes in accurate molecular masses and fragment ions with those of parent compounds. A total of five parent active compounds (BHCl, emodin, Rg1, Rb1 and Re) and 10 metabolites were found from the rat plasma 2 h after oral administration of HNYCF dosage, of which two metabolites of emodin were observed for the first time. The proposed metabolic pathways of the bioactive components in the rat plasma are helpful for further studies on the pharmacokinetics and real active compound forms of this drug.

  17. The classification method of syndromes in the Bei ji qian jin yao fang (Essential Recipes for Emergent Use Worth A Thousand Gold)%《备急千金要方》病证分类方法

    Institute of Scientific and Technical Information of China (English)

    申晓伟; 郑文杰; 马明越; 张志斌

    2014-01-01

    Bei ji qian jin yao fang (Essential Recipes for Emergent Use Worth A Thousand Gold) epitomizes the medical achievements of the Tang Dynasty with an extensive coverage.The classification method of syndromes in this book is derived from Neijing (Inner Canon),Nanjing (Classic of Questioning),Shang han za bing lun (Treatise on Cold Pathogenic and Miscellaneous Diseases),Zhu bing yuan hou lun (General Treatise on Causes and Manifestations of All Diseases),and enlightens the later generation with many unique characteristics,reflecting the mature recognition of the diseases in the contemporary clinical medicine from one side.It is still of great significance for understanding the diseases and exploring its pathogenesis even today.%《备急千金要方》为唐代医书集大成者,内容涵盖十分广泛,其病证分类方法,上承《内经》、《难经》、《伤寒杂病论》、《诸病源候论》,下启后世诸家,有很多独创之处,从一个侧面体现出当时的临床医学对很多疾病的认识已相当成熟,直到现代,对于今人认识疾病,探讨疾病病机仍有很大的意义.

  18. Examination of spotted sand bass (Paralabrax maculatofasciatus) pollutant bioaccumulation in San Diego Bay, San Diego, California.

    Science.gov (United States)

    Loflen, Chad L

    2013-01-01

    The spotted sand bass (Paralabrax maculatofasciatus) is an important recreational sport and subsistence food fish within San Diego Bay, a large industrialized harbor in San Diego, California. Despite this importance, few studies examining the species life history relative to pollutant tissue concentrations and the consumptive fishery exist. This study utilized data from three independent spotted sand bass studies from 1989 to 2002 to investigate PCB, DDT, and mercury tissue concentrations relative to spotted sand bass age and growth in San Diego Bay, with subsequent comparisons to published pollutant advisory levels and fishery regulations for recreational and subsistence consumption of the species. Subsequent analysis focused on examining temporal and spatial differences for different regions of San Diego Bay. Study results for growth confirmed previous work, finding the species to exhibit highly asymptotic growth, making tissue pollutant concentrations at initial take size difficult if not impossible to predict. This was corroborated by independent tissue concentration results for mercury, which found no relationship between fish size and pollutant bioaccumulation observed. However, a positive though highly variable relationship was observed between fish size and PCB tissue concentration. Despite these findings, a significant proportion of fish exhibited pollutant levels above recommended state recreational angler consumption advisory levels for PCBs and mercury, especially for fish above the minimum take size, making the necessity of at-size predictions less critical. Lastly, no difference in tissue concentration was found temporally or spatially within San Diego Bay.

  19. San Bernardino National Wildlife Refuge Well 10

    Energy Technology Data Exchange (ETDEWEB)

    Ensminger, J.T.; Easterly, C.E.; Ketelle, R.H.; Quarles, H.; Wade, M.C.

    1999-12-01

    The U.S. Geological Survey (USGS), at the request of the U.S. Fish and Wildlife Service, evaluated the water production capacity of an artesian well in the San Bernardino National Wildlife Refuge, Arizona. Water from the well initially flows into a pond containing three federally threatened or endangered fish species, and water from this pond feeds an adjacent pond/wetland containing an endangered plant species.

  20. Bismuth ochers from San Diego Co., California

    Science.gov (United States)

    Schaller, W.T.

    1911-01-01

    The chief points brought out in this paper may be briefly summarized as follows: (1) The existence of natural Bi2O3 has not been established. (2) Natural bismite or bismuth ocher, when pure, is more probably a bismuth hydroxide. (3) The bismuth ochers from San Diego County, California, are either a bismuth hydroxide or bismuth vanadate, pucherite, or mixtures of these two. (4) Pucherite has been found noncrystallin and determined for the first time in the United States.

  1. The San Joaquin Valley Westside Perspective

    Energy Technology Data Exchange (ETDEWEB)

    Quinn, Nigel W.T.; Linneman, J. Christopher; Tanji, Kenneth K.

    2006-03-27

    Salt management has been a challenge to westside farmerssince the rapid expansion of irrigated agriculture in the 1900 s. Thesoils in this area are naturally salt-affected having formed from marinesedimentary rocks rich in sea salts rendering the shallow groundwater,and drainage return flows discharging into the lower reaches of the SanJoaquin River, saline. Salinity problems are affected by the importedwater supply from Delta where the Sacramento and San Joaquin Riverscombine. Water quality objectives on salinity and boron have been inplace for decades to protect beneficial uses of the river. However it wasthe selenium-induced avian toxicity that occurred in the evaporationponds of Kesterson Reservoir (the terminal reservoir of a planned but notcompleted San Joaquin Basin Master Drain) that changed public attitudesabout agricultural drainage and initiated a steady stream ofenvironmental legislation directed at reducing non-point source pollutionof the River. Annual and monthly selenium load restrictions and salinityand boron Total Maximum Daily Loads (TMDLs) are the most recent of thesepolicy initiatives. Failure by both State and Federal water agencies toconstruct a Master Drain facility serving mostly west-side irrigatedagriculture has constrained these agencies to consider only In-Valleysolutions to ongoing drainage problems. For the Westlands subarea, whichhas no surface irrigation drainage outlet to the San Joaquin River,innovative drainage reuse systems such as the Integrated Farm DrainageManagement (IFDM) offer short- to medium-term solutions while morepermanent remedies to salt disposal are being investigated. Real-timesalinity management, which requires improved coordination of east-sidereservoir releases and west-side drainage, offers some relief toGrasslands Basin farmers and wetland managers - allowing greater salinityloading to the River than under a strict TMDL. However, currentregulation drives a policy that results in a moratorium on all

  2. Border Security: The San Diego Fence

    Science.gov (United States)

    2007-05-23

    sector is located north of Tijuana and Tecate, Mexican cities with a combined population of 2 million people, and features no natural barriers to entry...more marked in the areas where fencing was constructed within San Diego sector. The USBP’s Imperial Beach and Chula Vista stations saw their...effects on (1) the Tijuana River National Estuarine Research and Reserve; (2) state and federally listed threatened and endangered species; (3) lands

  3. An overview of San Francisco Bay PORTS

    Science.gov (United States)

    Cheng, Ralph T.; McKinnie, David; English, Chad; Smith, Richard E.

    1998-01-01

    The Physical Oceanographic Real-Time System (PORTS) provides observations of tides, tidal currents, and meteorological conditions in real-time. The San Francisco Bay PORTS (SFPORTS) is a decision support system to facilitate safe and efficient maritime commerce. In addition to real-time observations, SFPORTS includes a nowcast numerical model forming a San Francisco Bay marine nowcast system. SFPORTS data and nowcast numerical model results are made available to users through the World Wide Web (WWW). A brief overview of SFPORTS is presented, from the data flow originated at instrument sensors to final results delivered to end users on the WWW. A user-friendly interface for SFPORTS has been designed and implemented. Appropriate field data analysis, nowcast procedures, design and generation of graphics for WWW display of field data and nowcast results are presented and discussed. Furthermore, SFPORTS is designed to support hazardous materials spill prevention and response, and to serve as resources to scientists studying the health of San Francisco Bay ecosystem. The success (or failure) of the SFPORTS to serve the intended user community is determined by the effectiveness of the user interface.

  4. Magnetotelluric Data, Southern San Luis Valley, Colorado

    Science.gov (United States)

    Williams, Jackie M.; Rodriguez, Brian D.

    2007-01-01

    Introduction The population of the San Luis Valley region is growing rapidly. The shallow unconfined and the deeper confined Santa Fe Group aquifer in the San Luis Basin is the main sources of municipal water for the region. Water shortfalls could have serious consequences. Future growth and land management in the region depend on accurate assessment and protection of the region's ground-water resources. An important issue in managing the ground-water resources is a better understanding of the hydrogeology of the Santa Fe Group and the nature of the sedimentary deposits that fill the Rio Grande rift, which contain the principal ground-water aquifers. The U.S. Geological Survey (USGS) is conducting a series of multidisciplinary studies of the San Luis Basin located in southern Colorado. Detailed geologic mapping, high-resolution airborne magnetic surveys, gravity surveys, an electromagnetic survey, called magnetotellurics (MT), and hydrologic and lithologic data are being used to better understand the aquifer systems. The primary goal of the MT survey is to map changes in electrical resistivity with depth that are related to differences in rock type. These various rock types help control the properties of aquifers in the region. This report does not include any interpretation of the data. Its purpose is to release the MT data acquired at the 22 stations shown in figure 1.

  5. Magnetotelluric Data, San Luis Valley, Colorado

    Science.gov (United States)

    Rodriguez, Brian D.; Williams, Jackie M.

    2008-01-01

    The San Luis Valley region population is growing. Water shortfalls could have serious consequences. Future growth and land management in the region depend on accurate assessment and protection of the region?s ground-water resources. An important issue in managing the ground-water resources is a better understanding of the hydrogeology of the Santa Fe Group and the nature of the sedimentary deposits that fill the Rio Grande rift, which contain the principal ground-water aquifers. The shallow unconfined aquifer and the deeper confined Santa Fe Group aquifer in the San Luis Basin are the main sources of municipal water for the region. The U.S. Geological Survey (USGS) is conducting a series of multidisciplinary studies of the San Luis Basin located in southern Colorado. Detailed geologic mapping, high-resolution airborne magnetic surveys, gravity surveys, an electromagnetic survey (called magnetotellurics, or MT), and hydrologic and lithologic data are being used to better understand the aquifers. The MT survey primary goal is to map changes in electrical resistivity with depth that are related to differences in rock types. These various rock types help control the properties of aquifers. This report does not include any data interpretation. Its purpose is to release the MT data acquired at 24 stations. Two of the stations were collected near Santa Fe, New Mexico, near deep wildcat wells. Well logs from those wells will help tie future interpretations of this data with geologic units from the Santa Fe Group sediments to Precambrian basement.

  6. Research on SF/PEI self-assembly nano fiber membrane for filtering Cu2+%SF/PEI自组装纳米纤维膜用于Cu2+过滤的研究

    Institute of Scientific and Technical Information of China (English)

    马瑞丽; 何建新; 张弦

    2012-01-01

    采用静电层层自组装技术将丝素(SF)与聚乙烯亚胺(PEI)复合制备的SF/PEI纳米纤维,随着自组装层数的增多,纤维直径变粗,表面变得不规整,且在一定的自组装层数内,复合纳米纤维膜对Cu2+的过滤效率不断增大.杂化Fe后,纤维表面变得更加凹凸不平,但对Cu2+的过滤效果显著改善.这对于制备新型、高效、无二次污染、低治理成本的替代或改进的重金属过滤材料提供了理论意义.%F/PEI composite Nano fiber membrane was made in this experiment by layer-layer electrostatic self-assembly technology, With the increasing in the number in self-assembly layer, the fiber diameter become larger, the surface become irregular, and in certain layer, the adsorption efficiency of Cu2+ increases. After the hybrid Fe, fiber surface become more uneven, but the adsorption efficiency improved significantly. This experiment provides theoretical significance for new, effective, no secondary pollution and low management cost alternative or improved heavy metal filter material.

  7. 大川芎方多元释药系统的体外释药特征和大鼠体内药动学研究%Release characteristics in vitro and pharmacokinetics of Da Chuanxiong Fang multiunit drug delivery system in rats

    Institute of Scientific and Technical Information of China (English)

    魏元锋; 张宁; 林晓; 冯怡

    2011-01-01

    The drug release characteristics of Da Chuanxiong Fang multiunit drug delivery system (DCXFMDDS) in vivo and in vitro were evaluated. Ferulic acid (FA) and senkyunolide I (SI) were used as marker components, which were two of the effective components of Da Chuanxiong Fang. And their contents were determined by HPLC. Drug release characteristics in vitro of DCXFMDDS and Da Chuanxiong pills and pharmacokinetics characteristics of DCXFMDDS and Da Chuanxiong Fang active fraction (DCXFAF) in rats were compared. It was obvious that FA released from the DCXFMDDS in a sustained fashion but SI in a fast fashion both in vitro and in vivo. The releasing process and the releasing mechanism of FA and SI from DCXFMDDS were different, but the AUC value indicated that compared with DCXFAF the extent of absorption of FA and SI from DCXFMDDS was increased. Though from the same multiunit drug delivery system, FA an SI had different drug release characteristics both in vitro and in vivo, and that may be one of the reason why DCXFMDDS has the good properties such as rapid and long-lasting effect and high efficiency.%本文评价了大川芎方多元释药系统(Da Chuanxiong Fang multiunit drug delivery system,DCXFMDDS)体内外释药特点.以已知的有效成分阿魏酸(ferulic acid,FA)与洋川芎内酯I(senkyunolide I,SI)为指标成分,采用HPLC法测定其含量,比较了DCXFMDDS与大川芎片的体外释药特征,以及与大川芎方效应组分(Da Chuanxiong Fang active fraction,DCXFAF)的大鼠体内药动学特征.结果表明,DCXFMDDS中FA在体内外具有明显的缓释作用,而SI在体内外均能快速释药,两种物质体外释药特征及其机制均不相同,但体内吸收程度(以AUC表征)均好于DCXFAF组.DCXFMDDS改变了不同化学成分在体内外释药特征,可能是其起效快、药效好、药效维持时间长的原因之一.

  8. Historia del Estadio San Marcos de la Universidad Nacional Mayor de San Marcos

    OpenAIRE

    Meza Bazán, Mario Miguel

    2009-01-01

    La historia del Estadio San Marcos de la Universidad San Marcos de Lima es pretexto para estudiar sus precarias relaciones con el Estado en contextos de autoritarismo y clientelismo. El estadio, planeado al principio para ser el mayor coliseo deportivo de Perú, se convirtió con la donación del dictador Manuel Odría a la Universidad, en espacio de su futura ciudad universitaria. Señalamos que esta donación se hizo cuando el estadio no había sido concluido porque le resultaba demasiado oneroso ...

  9. A Study of the San Andreas Slip Rate on the San Francisco Peninsula, California

    Science.gov (United States)

    Feigelson, L. M.; Prentice, C.; Grove, K.; Caskey, J.; Ritz, J. F.; Leslie, S.

    2008-12-01

    The most recent large earthquake on the San Andreas Fault (SAF) along the San Francisco Peninsula was the great San Francisco earthquake of April 18, 1906, when a Mw= 7.8 event ruptured 435-470 km of the northern SAF. The slip rate for this segment of the SAF is incompletely known but is important for clarifying seismic hazard in this highly urbanized region. A previous study south of our site has found an average slip rate of 17±4 mm/yr for the late Holocene on the San Francisco Peninsula segment of the SAF. North of the Golden Gate, the SAF joins the San Gregorio Fault with an estimated slip rate of 6 mm/yr. A trench study north of where the two faults join has produced an average late Holocene slip rate of 24±3 mm/yr. To refine slip-rate estimates for the peninsula segment of the SAF, we excavated a trench across the fault where we located an abandoned channel between the San Andreas and Lower Crystal Springs reservoirs. This abandoned channel marks the time when a new channel cut across the SAF; the new channel has since been offset in a right-lateral sense about 20 m. The measured amount of offset and the age of the youngest fluvial sediments in the abandoned channel will yield a slip rate for the San Francisco Peninsula segment of the SAF. We excavated a trench across the abandoned channel and logged the exposed sediments. Our investigation revealed channel-fill alluvium incised and filled by probable debris flow sediments, and a wide fault zone in bedrock, west of the channel deposits. The most prominent fault is probably the strand that moved in 1906. We completed a total-station survey to more precisely measure the offset stream, and to confirm that the fault exposed in the trench aligns with a fence that is known to have been offset 2.8m during the 1906 earthquake. We interpret the debris flow sediments to represent the last phase of deposition prior to abandonment of the old channel. We collected samples for radiocarbon dating, optically stimulated

  10. SSC San Diego Command History Calendar Year 2005

    Science.gov (United States)

    2006-03-01

    SSC San Diego from Capt. Tim Flynn on 18 August 2005. Capt. Unetic joined the Center from SPAWAR Headquarters where he was Executive Assistant to...communications. Rear Adm. Tim Flynn Rear Adm. Tim Flynn served as SSC San Diego Commanding Officer beginning 2 May 2002. Before joining SSC San Diego, he was...Steven Short, Arleen Simbulan, Robert Smith, Gleason Snashall, Dow Street, Weden Teng, Deborah Tharp , Tine Thompson, Viet Tran, Thomas Tucker, Rob Turner

  11. Geophysical evidence for wedging in the San Gorgonio Pass structural knot, southern San Andreas fault zone, southern California

    Science.gov (United States)

    Langenheim, V.E.; Jachens, R.C.; Matti, J.C.; Hauksson, E.; Morton, D.M.; Christensen, A.

    2005-01-01

    Geophysical data and surface geology define intertonguing thrust wedges that form the upper crust in the San Gorgonio Pass region. This picture serves as the basis for inferring past fault movements within the San Andreas system, which are fundamental to understanding the tectonic evolution of the San Gorgonio Pass region. Interpretation of gravity data indicates that sedimentary rocks have been thrust at least 5 km in the central part of San Gorgonio Pass beneath basement rocks of the southeast San Bernardino Mountains. Subtle, long-wavelength magnetic anomalies indicate that a magnetic body extends in the subsurface north of San Gorgonio Pass and south under Peninsular Ranges basement, and has a southern edge that is roughly parallel to, but 5-6 km south of, the surface trace of the Banning fault. This deep magnetic body is composed either of upper-plate rocks of San Gabriel Mountains basement or rocks of San Bernardino Mountains basement or both. We suggest that transpression across the San Gorgonio Pass region drove a wedge of Peninsular Ranges basement and its overlying sedimentary cover northward into the San Bernardino Mountains during the Neogene, offsetting the Banning fault at shallow depth. Average rates of convergence implied by this offset are broadly consistent with estimates of convergence from other geologic and geodetic data. Seismicity suggests a deeper detachment surface beneath the deep magnetic body. This interpretation suggests that the fault mapped at the surface evolved not only in map but also in cross-sectional view. Given the multilayered nature of deformation, it is unlikely that the San Andreas fault will rupture cleanly through the complex structures in San Gorgonio Pass. ?? 2005 Geological Society of America.

  12. 2007 San Diego wildfires and asthmatics.

    Science.gov (United States)

    Vora, Chirag; Renvall, Marian J; Chao, Peter; Ferguson, Paul; Ramsdell, Joe W

    2011-02-01

    This case series reports the changes in the respiratory health of eight asthmatic subjects and the relationship with air quality associated with the October 2007 firestorm in San Diego County of California. Participants were eight subjects with asthma enrolled in Asthma Clinical Research Network (ACRN) (NIH# U10-HL074218) studies at the University of California San Diego (UCSD), School of Medicine, who had study data collected immediately prior, during and 1 month after the 5-day firestorm in San Diego County. Air quality deteriorated to an extreme average of 71.5 mg/m(3) small particulate matter less than 2.5 μm (PM(2.5)) during the firestorm. Respiratory health data included morning and evening peak expiratory flow rates (PEFR), morning and evening Forced Expiratory Volume in one second (FEV(1)), rescue medication usage, and sputum eosinophils. Morning and evening PEFR and FEV(1) rates remained stable. The two subjects tested during the fires had elevated eosinophil counts and rescue medication usage was increased in five of the eight subjects. Pulmonary function test values were stable during the wildfires for all eight subjects but there was a statistically significant increase in rescue medication usage during the wildfires that correlated with PM(2.5) values. The two subjects tested during the fires showed increases in sputum eosinophil counts consistent with increased airways inflammation. These findings suggest that poor air quality associated with wildfires resulted in an increase in airways inflammation in these asthmatic subjects, but pulmonary function tests remained stable, possibly due to increased rescue medication usage. This is especially pertinent as there is an increase in incidence of wildfires this decade.

  13. Dal "San Marco" al "Vega". (English Title: From "San Marco" to Vega)

    Science.gov (United States)

    Savi, E.

    2017-10-01

    Apart from the two superpowers, among the other countries Italy has had an important role in astronautics. The roots of Italian astronautics' history runs deep in the hottest years of the Cold War, and it had its first remarkable achievement in the San Marco project..after years of advanced technologies testing, they achieved European cooperation and built VEGA, the current Arianespace light launcher.

  14. College Success and the Black Male. San Jose City College, San Jose, California. Research Report #128.

    Science.gov (United States)

    Carr, Percy; And Others

    In 1992, a study was conducted at San Jose City College (SJCC) and Evergreen Valley College (EVC), California, to examine the fourth semester persistence rates of black male students and to investigate the effect of SJCC athletic and athlete academic support programs on persistence. Study findings included the following: (1) new full-time (NFT)…

  15. 78 FR 57482 - Safety Zone; America's Cup Aerobatic Box, San Francisco Bay, San Francisco, CA

    Science.gov (United States)

    2013-09-19

    ... government and the States, or on the distribution of power and responsibilities among the various levels of... Sector San Francisco; telephone (415) 399-3585 or email at D11-PF-MarineEvents@uscg.mil . If you have... ``America's Cup World Series'' regattas in 2012 and the ``Louis Vuitton Cup,'' ``Red Bull Youth America's...

  16. SANS-II at SINQ: Installation of the former Risø-SANS facility

    DEFF Research Database (Denmark)

    Strunz, P.; Mortensen, K.; Janssen, S.

    2004-01-01

    SANS-II facility at SINQ (Paul Scherrer Institute)-the reinstalled former Riso small-angle neutron scattering instrument-is presented. Its operational characteristics are listed. Approaches for precise determination of wavelength, detector dead time and attenuation factors are described as well. (C...

  17. Otomi de San Andres Cuexcontitlan, Estado de Mexico (Otomi of San Andres Cuexcontitlan, State of Mexico).

    Science.gov (United States)

    Lastra, Yolanda

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Otomi, an indigenous language of Mexico spoken in San Andres Cuexcontitlan, in the state of Mexico. The objective of collecting such a representative…

  18. 75 FR 15611 - Safety Zone; United Portuguese SES Centennial Festa, San Diego Bay, San Diego, CA

    Science.gov (United States)

    2010-03-30

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; United Portuguese SES Centennial Festa, San... United Portuguese SES Centennial Festa. This temporary safety zone is necessary to provide for the safety... Spectaculars is sponsoring the United Portuguese SES Centennial Festa, which will include a fireworks...

  19. EPA, San Diego County Air District to Unveil Air Monitor in San Ysidro

    Science.gov (United States)

    LOS ANGELES - On Tuesday, U.S. EPA Regional Administrator Jared Blumenfeld, along with representatives from the San Diego County Air Pollution Control District (SDAPCD), U.S. Customs and Border Protection and the U.S. General Services Administration, will

  20. Chinanteco de San Juan Lealao, Oaxaca (Chinantec of San Juan Lealao, Oaxaca).

    Science.gov (United States)

    Mexico Coll. (Mexico City)

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Chinantec, an indigenous language of Mexico spoken in San Juan Lealao, in the state of Oaxaca. The objective of collecting such a representative sampling of…

  1. Huave de San Mateo del Mar, Oaxaca (Huave of San Mateo del Mar, Oaxaca).

    Science.gov (United States)

    Mexico Coll. (Mexico City)

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Huave, an indigenous language of Mexico spoken in San Mateo del Mar, in the state of Oaxaca. The objective of collecting such a representative sampling of…

  2. Trique de San Juan Copala, Oaxaca (Trique of San Juan Copala, Oaxaca).

    Science.gov (United States)

    Mexico Coll. (Mexico City)

    This document is one of 17 volumes on indigenous Mexican languages and is the result of a project undertaken by the Archivo de Lenguas Indigenas de Mexico. This volume contains information on Trique, an indigenous language of Mexico spoken in San Juan Copala, in the state of Oaxaca. The objective of collecting such a representative sampling of the…

  3. San Francisco Bay National Wildlife Refuge Complex Annual Narrative Report 1979

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The San Francisco Bay National Wildlife Refuge Complex comprises of three refuges including the San Francisco Bay National Wildlife Refuge, the San Pablo Bay...

  4. San Francisco Bay National Wildlife Refuge Complex Annual Narrative Report 1983

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The San Francisco Bay National Wildlife Refuge Complex comprises of three refuges including the San Francisco Bay National Wildlife Refuge, the San Pablo Bay...

  5. San Francisco Bay National Wildlife Refuge Complex Annual Narrative Report 1981

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The San Francisco Bay National Wildlife Refuge Complex comprises of three refuges including the San Francisco Bay National Wildlife Refuge, the San Pablo Bay...

  6. San Francisco Bay National Wildlife Refuge Complex Annual Narrative Report 1977

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The San Francisco Bay National Wildlife Refuge Complex comprises of three refuges including the San Francisco Bay National Wildlife Refuge, the San Pablo Bay...

  7. [Health levels in San Andres Cholula].

    Science.gov (United States)

    Alvarez Martinez, A; Corro Fernandez, G; Balmaceda, M

    1991-12-01

    In matters of health and curing, the community of San Andres Cholula in Puebla, Mexico, demonstrates a syncretism similar to religious syncretism. Perspectives on illness and health consistent with the traditional medical practices of curanderos coexist with modern medical practices. Curanderos and physicians often treat the same patients. A curandero's powers are viewed as a special gift transmitted by God or the saints during a dream. The curandero effects a cure not only through knowledge of the medicinal plants, rites, and ceremonies, but by understanding the context of the patient. The Western medical concept of disease emphasizes a biological model and technological control, to the detriment of mental, behavioral, and social factors and determinants. The traditional medical concept stresses the relationship of the individual to the social and ecological environment. Improvements in life expectancy in the developing countries in recent years have been attributed to improved levels of living or to importation of vaccination programs, antibiotics, and similar technologies from the developed countries. The vital register of San Andres Cholula records many deaths whose cause cannot be easily interpreted according to the World Health Organization International Classification of Diseases. It is clear, however, that the root cause of many deaths is malnutrition. The proportion of deaths caused by infectious diseases has declined in Mexico since 1940, but Puebla is still included among the states with the highest incidence. There are great regional and rural-urban mortality differentials in Mexico. In the past 50 years, the infant mortality rate has declined from 250 to 40/1000 live births in San Andres Cholula, more as a result of vaccination campaigns than of improved levels of living. 89% of children have been vaccinated, but the population still lives in about the same state of material comfort as it has for generations except that most households have televisions

  8. Gredos San Diego Cooperative. Cooperate to undertake

    Directory of Open Access Journals (Sweden)

    Carlos de la Higuera

    2012-12-01

    Full Text Available This paper analyzes the cooperative experience through Gredos San Diego model, its institutional approaches and its history from the point of view of management, focusing on the variables that enable the success of a collective ownership institution. First, the author makes a brief analysis of the principles that guide the cooperative, its origins and its current situation, including the development of GSD Cooperative Group. It continues exploring the evolution of management, dividing it into four distinct stages, and concludes with a summary with the findings of the previous president of the cooperative.Received: 23.07.2012Accepted: 10.09.2012

  9. PLAN DIRECTOR URBANO DE SAN JOSÉ

    OpenAIRE

    Municipalidad de San José, Gobierno Local

    2011-01-01

    Este documento  resume el Plan Director Urbano del Cantòn Central de San José. Este Plan Urbano-Territorial ha sido diseñado como respuesta concreta del Gobierno Local, a la necesidad de regular y planificar el desarrollo general de la ciudad y el proceso de urbanización. Se incluye en un primer apartado una caracterización de la problemática y las tendencias urbanas josefinas; un segundo contenido se definen ls objetivos y líneas estratégicas de la ciudad que queremos. Un tercer apartado sob...

  10. Early Maya writing at San Bartolo, Guatemala.

    Science.gov (United States)

    Saturno, William A; Stuart, David; Beltrán, Boris

    2006-03-03

    The ruins of San Bartolo, Guatemala, contain a sample of Maya hieroglyphic writing dating to the Late Preclassic period (400 B.C. to 200 A.D.). The writing appears on preserved painted walls and plaster fragments buried within the pyramidal structure known as "Las Pinturas," which was constructed in discrete phases over several centuries. Samples of carbonized wood that are closely associated with the writing have calibrated radiocarbon dates of 200 to 300 B.C. This early Maya writing implies that a developed Maya writing system was in use centuries earlier than previously thought, approximating a time when we see the earliest scripts elsewhere in Mesoamerica.

  11. Rhythm & Blues de sans papiers parisiens

    OpenAIRE

    Fogel, Frederique

    2014-01-01

    Le sans papiers (ici parisien) est l’une des figures, plurielles, du migrant immobilisé. Le temps qui passe lui ouvre des possibilités de régulariser sa situation. Il lui faut donc patienter, s’adapter dans la contrainte au "rhythm" défini par l’administration, tantôt lent, tantôt rapide. Et trouver aussi son propre rythme en posant des actes. Il lui faut faire avec le blues, qui survient souvent par la prise de conscience du décalage entre l’espoir d’une vie meilleure qui avait déclenché le ...

  12. 基于网络药理学研究大川芎方治疗偏头痛的作用机制%Network-assisted Investigation into Mechanism of Dachuanxiong Fang on Migraine

    Institute of Scientific and Technical Information of China (English)

    袁荣高; 顾明华; 古江勇

    2016-01-01

    目的:研究大川芎方治疗偏头痛的分子作用机制。方法结合文献挖掘、分子对接和网络分析研究大川芎方中化合物群与偏头痛相关靶蛋白的相互作用。结果分子对接发现大川芎中有38个活性成分,且大部分具有较好的口服生物利用度和良好的脑内暴露量,进一步网络分析发现 senkyunolide M,riligustilide,levistolide A,chuanxiongterpene 等16个化合物为其主要活性成分,可通过与 CGRP、p38、TNF-α、iGluR5、NOS 等18个靶蛋白作用,抑制神经源性炎症和神经递质的产生,降低源自三叉神经的感受器对伤害性信息的超敏化,并对抗皮层扩布性抑制,从而发挥治疗偏头痛的作用。结论通过网络分析明确了大川芎方治疗偏头痛的分子作用机制,诠释了其科学内涵,有助于推动后续相应制剂的药效物质基础研究和分子机制实验研究。%OBJECTIVE To understand the mechanism of Dachuanxiong Fang on migraine.METHODS Data mining,Mo-lecular docking and Network Analysis were employed to investigate the active compounds and key target protein.RESULTS 38 active hits were identified by virtual screening,of which,most compounds had good oral bioavailability and might be opti-mal CNS exposure.Among them,1 6 molecules(senkyunolide M,levistolide A,chuanxiongterpene,riligustilide,etc.) could significantly contribute to alleviate migraine.And they could target 18 protein(such as CGRP,p38,TNF-α,iGluR5,NOS)to inhibit neurogenic inflammation and neutrotransmitters,to reduce the sensitive of nociceptors originated in the trigeminal gan-glion,and to combat the genesis and propagation of cortical spreading depression.CONCLUSION It was illustrated the molec-ular mechanism and the key active compounds of Dachuanxiong Fang,which was helpful for wet experiments to explore targets and active compounds.

  13. Application of the donkey-hide gelatin in gynecology diseases in the Qian Jin Fang%阿胶在《千金方》妇科疾病中的应用

    Institute of Scientific and Technical Information of China (English)

    王静; 王晓明; 田思胜

    2012-01-01

    Woman has the special physiological function, which determines different cure methods when they are ill. Catamenia, pregnancy, childbearing and lactation are all related with blood. The donkey-hide gelatin is regularly used in clinical practice with its function of regulating blood. It is a common gynecological herb in treatment of gynecological diseases, for the good effect of nourishing blood for moistening dryness and nourishing blood for preventing miscarriage. Because it is the sentient flesh thing, the donkey-hide gelatin can nourish Qi and blood, consolidate Chong vessel well in postpartum. This paper discusses the usage of donkey-hide gelatin in Qian Jin Fang which was written by SUN Si-miao for providing reference and inspiration in modern clinical and medical research.%妇人特殊的生理现象,决定了其治疗时立方有别.妇女经、孕、产、乳的生理活动均以血为用,而阿胶是治疗或调养时常用理血之药,同时为妇科疾病常用药,是养血润燥佳品,是养血安胎圣药,是产后调养气血,血肉有情之物,为养血固冲之上品.文章通过对阿胶在《千金方》中的运用浅析,以期阿胶在现代临床应用和医学研究中提供借鉴和启迪.

  14. Qualitative and quantitative analysis of chemical constituents in traditional Chinese medicinal formula Tong-Xie-Yao-Fang by high-performance liquid chromatography/diode array detection/electrospray ionization tandem mass spectrometry.

    Science.gov (United States)

    Yan, Zhixiang; Yang, Xinghao; Wu, Jianbo; Su, Huai; Chen, Chen; Chen, Yin

    2011-04-08

    Tong-Xie-Yao-Fang (TXYF), a famous traditional Chinese medicine formula, has efficient effects on treatment of the diarrhea-predominant irritable bowel syndrome (D-IBS), a disease with high incidence worldwide. However, the active principles for this complex formula have not been fully explored so far. In this paper, high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) was applied for the qualitative and quantitative analysis of major chemical constituents in TXYF. Two monoterpene glycosides, one chromone and five polymethoxylated flavones were tentatively characterized based on the retention times, UV spectra and MS data. Fifteen compounds were unambiguously identified by comparison with reference standards. Constituents such as lactone and steroidal, which could not be found by single HPLC method due to the low content in the formula, were identified in this paper. Seven compounds (gallic acid, prim-O-β-D-glucosylcimifugin, paeoniflorin, cimifugin, naringin, hesperidin and 4'-O-β-D-glucosyl-5-O-methylvisamminol) were quantified by HPLC-DAD using a C18 column and gradient elution with acetonitrile and water-0.1% formic acid. The method exhibited intra- and inter-day precision of less than 2.35% and 3.14%, respectively. The LODs and the LOQs for the analytes were less than 0.47 and 1.82 μg ml(-1), respectively. The overall recoveries ranged from 96.82% to 102.47%, with the R.S.D. ranging from 1.17% to 3.94%. These results demonstrated that our present method was effective and reliable for comprehensive quality evaluation of TXYF. Meanwhile, the study might provide the chemical evidence for revealing the material basis of its therapeutic effects. Copyright © 2011 Elsevier B.V. All rights reserved.

  15. Arabic and Persian Sources used in Liu Zhi' s Tian-Fang Xing-Li%刘智《天方性理》采辑经书文献考

    Institute of Scientific and Technical Information of China (English)

    姚继德; 王根明

    2012-01-01

    Liu Zhi (1660--1730) is one eminent Chinese Muslim philosopher in the earlier Qing dynasty. The Arabic and Persian bibliography (Caijijingshumu) attached his Islamic philosophical translation work Tian-Fang Xing-Li, is the oldest literature recorded in Arabic and Persian transliteration in China. The list of his reference contains important information of religious and cultural exchanges between China and the Islamic world before 18th century. The paper offers us a new comprehensive textual research of this bibliography based on the Arabic and Per-sian sources survived in Chinese Muslim society as well as the sources in Arab and Persian world.%刘智(1660—1730)是清初中国伊斯兰学者,其著作《天方性理》卷首附有"采辑经书目",该书目是中国现存最早的阿拉伯文和波斯文文献目录。本文根据中国回族经堂教育现存的阿拉伯文和波斯文文献,并参考国外阿拉伯文和波斯文文献资料对之作了较为全面的考证,是迄今为止对《天方性理》参考文献的全面考释,对研究古代伊斯兰世界与中国穆斯林社会的文化交流有重要文献学价值。

  16. 子宫腔填塞纱条联合欣母沛治疗产后出血的疗效%Effect of Uterine Cavity Gauze Packing and Yan Pei Joint Treatment on Post-partum Hemorrhage

    Institute of Scientific and Technical Information of China (English)

    伍志虹; 魏静

    2009-01-01

    Objective To explore the clinical effect of uterine cavity gauze packing and yan pei joint treatment on post-partum haemorrhage.Methods 118 post-partum hemorrhage patients were randomly divided into 2 groups:control group (n=51) and treatment group (n=67).The control group were treated with uterine massage,intravenous administration of oxytocin,and sublingual administration of misoprostol;As to the treatment group,uterine cavity gauze packing with yan pei intramuscular injection was jointly administered in addition to the same treatment for the control group.The status of post-partum hemorrhage was compared between the two groups.Results Bleeding of the treatment group was significantly less than that of the control group(P<0.05,respectively) either 2 hours or 24 hours after treatment.Conclusion Uterine cavity gauze packing and yan pei joint treatment is an effective one for post-partum hemorrhage.%目的 探讨子宫腔填塞纱条联合欣母沛治疗产后出血的临床疗效.方法 将118例产后出血患者随机分为2组:对照组51例和治疗组67例.对照组采用按摩子宫、注射催产素、舌下含服米索前列醇等治疗,治疗组在对照组治疗的基础上加用欣母沛联合子宫腔填塞纱条治疗.并比较2组产后出血的情况.结果 治疗组治疗后2、24 h出血量均明显少于对照组(均P<0.05).结论 欣母沛联合子宫腔填塞纱条是治疗产后出血的一种有效方法.

  17. 33 CFR 80.1142 - San Francisco Harbor, CA.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false San Francisco Harbor, CA. 80.1142 Section 80.1142 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Coast § 80.1142 San Francisco Harbor, CA. A straight...

  18. 33 CFR 80.1104 - San Diego Harbor, CA.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false San Diego Harbor, CA. 80.1104 Section 80.1104 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Coast § 80.1104 San Diego Harbor, CA. A line drawn...

  19. 33 CFR 80.1130 - San Luis Obispo Bay, CA.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false San Luis Obispo Bay, CA. 80.1130 Section 80.1130 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Coast § 80.1130 San Luis Obispo Bay, CA. A line drawn...

  20. 1906 Letter to the San Francisco Health Department

    Science.gov (United States)

    Schmachtenberg, Kristin

    2006-01-01

    On Wednesday, April 18, 1906, an earthquake, measuring 7.8 on the Richter magnitude scale and lasting 48 seconds, erupted along the San Andreas fault with a flash point originating in the San Francisco Bay area. The force of the earthquake tore apart buildings and roads, causing water and gas mains to twist and break. The resulting effects of the…

  1. The San Diego Panasonic Partnership: A Case Study in Restructuring.

    Science.gov (United States)

    Holzman, Michael; Tewel, Kenneth J.

    1992-01-01

    The Panasonic Foundation provides resources for restructuring school districts. The article examines its partnership with the San Diego City School District, highlighting four schools that demonstrate promising practices and guiding principles. It describes recent partnership work on systemic issues, noting the next steps to be taken in San Diego.…

  2. Voice and Valency in San Luis Potosi Huasteco

    Science.gov (United States)

    Munoz Ledo Yanez, Veronica

    2014-01-01

    This thesis presents an analysis of the system of transitivity, voice and valency alternations in Huasteco of San Luis Potosi (Mayan) within a functional-typological framework. The study is based on spoken discourse and elicited data collected in the municipalities of Aquismon and Tancanhuitz de Santos in the state of San Luis Potosi, Mexico. The…

  3. The San Diego Panasonic Partnership: A Case Study in Restructuring.

    Science.gov (United States)

    Holzman, Michael; Tewel, Kenneth J.

    1992-01-01

    The Panasonic Foundation provides resources for restructuring school districts. The article examines its partnership with the San Diego City School District, highlighting four schools that demonstrate promising practices and guiding principles. It describes recent partnership work on systemic issues, noting the next steps to be taken in San Diego.…

  4. Reconstructing a Spanish Mission: San Luis de Talimali.

    Science.gov (United States)

    McEwan, Bonnie G.; Hann, John H.

    2000-01-01

    Focuses on the reconstruction of the San Luis de Talimali, which was among the largest and most important missions in Spanish Florida with the Apalachee Indians as their parishioners. Describes the various structures found at San Luis: (1) the council house; (2) the chief's house; and (3) the church. (CMK)

  5. San Marino-China Friendship Association Delegation in China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    <正>On the occasion of the 20th anniversary of the San Marino-China Friendship Association (SMCFA), a 26-member delegation of the Association headed by G. F. Terenzi, president of the SMCFA and former captain regent of San Marino, visited China from May 16 to 28.

  6. COSMOS (County of San Mateo Online System). A Searcher's Manual.

    Science.gov (United States)

    San Mateo County Superintendent of Schools, Redwood City, CA. Educational Resources Center.

    Operating procedures are explained for COSMOS (County of San Mateo Online System), a computerized information retrieval system designed for the San Mateo Educational Resources Center (SMERC), which provides interactive access to both ERIC and a local file of fugitive documents. COSMOS hardware and modem compatibility requirements are reviewed,…

  7. 77 FR 47358 - San Juan National Forest Resource Advisory Committee

    Science.gov (United States)

    2012-08-08

    ... Forest Service San Juan National Forest Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The San Juan National Forest Resource Advisory Council (RAC) will meet in... recommendations to the Forest Service concerning projects and funding consistent with Title II of the Act. The...

  8. Wind resource assessment: San Nicolas Island, California

    Energy Technology Data Exchange (ETDEWEB)

    McKenna, E. [National Renewable Energy Lab., Golden, CO (United States); Olsen, T.L. [Timothy L. Olsen Consulting, (United States)

    1996-01-01

    San Nicolas Island (SNI) is the site of the Navy Range Instrumentation Test Site which relies on an isolated diesel-powered grid for its energy needs. The island is located in the Pacific Ocean 85 miles southwest of Los Angeles, California and 65 miles south of the Naval Air Weapons Station (NAWS), Point Mugu, California. SNI is situated on the continental shelf at latitude N33{degree}14` and longitude W119{degree}27`. It is approximately 9 miles long and 3.6 miles wide and encompasses an area of 13,370 acres of land owned by the Navy in fee title. Winds on San Nicolas are prevailingly northwest and are strong most of the year. The average wind speed is 7.2 m/s (14 knots) and seasonal variation is small. The windiest months, March through July, have wind speeds averaging 8.2 m/s (16 knots). The least windy months, August through February, have wind speeds averaging 6.2 m/s (12 knots).

  9. Solar sanitary system (SOL-SAN)

    Energy Technology Data Exchange (ETDEWEB)

    Cobb, J.C.

    1996-11-01

    Ordinary composting toilets, because of cooling by evaporation, do not heat the product (humus) hot enough to kill all pathogenic viruses, bacteria, or parasite eggs and cysts. The SOL-SAN system uses direct radiation to pasteurize incoming river water for drinking and also, separately, to pasteurize and dry the humus, and to pasteurize the effluent gray/brown water. Work is in progress on simple fool-proof methods of insuring that the water will not flow out unless it has been pasteurized. Heat exchangers recapture the heat from these very hot pasteurized liquids, thereby warming more in-coming water for washing, which is important for preventing transmission of pathogenic microbes. When pasteurized, the humus and gray/brown water can safely be recycled to fertilize and water the family vegetable garden. Thus no sewer would be needed, and the vegetables or fish would grow well. Widespread use of the SOL-SAN system would save water and nutrients, reduce the prevalence of infectious diseases, improve the nutrition and vitality of the population, and save the large fraction of human food now consumed by parasites.

  10. Synthetic seismicity for the San Andreas fault

    Directory of Open Access Journals (Sweden)

    S. N. Ward

    1994-06-01

    Full Text Available Because historical catalogs generally span only a few repetition intervals of major earthquakes, they do not provide much constraint on how regularly earthquakes recur. In order to obtain better recurrence statistics and long-term probability estimates for events M ? 6 on the San Andreas fault, we apply a seismicity model to this fault. The model is based on the concept of fault segmentation and the physics of static dislocations which allow for stress transfer between segments. Constraints are provided by geological and seismological observations of segment lengths, characteristic magnitudes and long-term slip rates. Segment parameters slightly modified from the Working Group on California Earthquake Probabilities allow us to reproduce observed seismicity over four orders of magnitude. The model yields quite irregular earthquake recurrence patterns. Only the largest events (M ? 7.5 are quasi-periodic; small events cluster. Both the average recurrence time and the aperiodicity are also a function of position along the fault. The model results are consistent with paleoseismic data for the San Andreas fault as well as a global set of historical and paleoseismic recurrence data. Thus irregular earthquake recurrence resulting from segment interaction is consistent with a large range of observations.

  11. San Joaquin-Tulare Conjunctive Use Model: Detailed model description

    Energy Technology Data Exchange (ETDEWEB)

    Quinn, N.W.T.

    1992-03-01

    The San Joaquin - Tulare Conjunctive Use Model (SANTUCM) was originally developed for the San Joaquin Valley Drainage Program to evaluate possible scenarios for long-term management of drainage and drainage - related problems in the western San Joaquin Valley of California. A unique aspect of this model is its coupling of a surface water delivery and reservoir operations model with a regional groundwater model. The model also performs salinity balances along the tributaries and along the main stem of the San Joaquin River to allow assessment of compliance with State Water Resources Control Board water quality objectives for the San Joaquin River. This document is a detailed description of the various subroutines, variables and parameters used in the model.

  12. San Pascual (2005) Año XLIII, n. 337

    OpenAIRE

    Pérez, María Dolores, O.S.C. (Directora)

    2005-01-01

    Editorial. Congreso eucarístico. ¡Con regocijo encontrada por un sencillo pastor!. Opúsculos de San Pascual. Recordando la vocación de Sor Mª Gracia. Eclesia de eucarestia II. San Pascual Patrono eucarístico II. Rincón poético. Vida en el santuario. Turnos de adoración diurna mensual en San Pascual. 2005 un año Sampascualino. Caminos de San Pascual. Año de la Inmaculada. Una imagen de San Pascual en México II. Crónica del año de la eucaristía. La luz de las imágenes. ¡Tu historia! ¡Mi histor...

  13. SAN MICHELE. ENTRE CIELO Y MAR / San Michele, between sky and sea

    Directory of Open Access Journals (Sweden)

    Pablo Blázquez Jesús

    2012-11-01

    Full Text Available ESUMEN El cementerio es uno de los tipos arquitectónicos más profundos y metafóricos. El concurso para la ampliación del cementerio de San Michele, convocado en 1998 por la administración Municipal de Venecia, se convierte en un excelente campo de pruebas sobre el que poder analizar el contexto histórico en torno a esta tipología, y su relación con la ciudad y el territorio. El estudio de este caso concreto nos permite descubrir personajes, relaciones casuales y hallazgos que se despliegan a lo largo del texto. La historia del cementerio de San Michele es también la crónica de la transformación de la ciudad de Venecia y su Laguna. Interpretando este concurso como un instrumento de investigación, el objetivo del artículo es el de comprender la realidad contemporánea de la arquitectura funeraria a través de la isla de San Michele, Venecia, y las propuestas finalistas de Carlos Ferrater, Enric Miralles y David Chipperfield. Una historia bajo la cual se vislumbran claves que nos sirven para reflexionar acerca del cementerio contemporáneo, la ciudad y el territorio.SUMMARY The cemetery is one of the most profound and metaphorical kinds of architecture. The competition for the extension of the San Michele Cemetery, called in 1998 by the Venice municipal administration, is an excellent testing ground on which to analyse the historical context surrounding this type of architecture, and its relationship with the city and the region. The study of this particular case allows us to uncover characters, casual relationships and findings that unfold throughout the text. The history of the San Michele cemetery is also the chronicle of the transformation of the city of Venice and its Lagoon. Interpreting this competition as a research tool, the aim of the paper is to understand the contemporary reality of funerary architecture through the island of San Michele, Venice, and the finalist proposals of Carlos Ferrater, Enric Miralles and David

  14. Combination of PEI-Mn0.5Zn0.5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma.

    Science.gov (United States)

    Tang, Qiusha; Lu, Mudan; Chen, Daozhen; Liu, Peidang

    2015-01-01

    To explore a new combination of thermal treatment and gene therapy for hepatoma, a heat-inducible herpes simplex virus thymidine kinase/ganciclovir (HSV-TK/GCV) gene therapy system was developed in which thermal energy generated by Mn0.5Zn0.5Fe2O4 nanoparticles (MZF-NPs) under an alternating magnetic field was used to activate gene expression. First, a recombinant eukaryotic plasmid, pHsp 70-HSV-TK, was constructed as a target gene for therapy. This recombinant plasmid was used to transfect SMMC-7721 hepatoma cells and the gene expression was evaluated. Magnet-induced heating was then applied to cells to assess the antihepatoma effects of the polyethylenimine (PEI)-MZF-NPs/pHsp 70-HSV-TK/GCV complex, in vitro and in vivo. The results showed that cells were successfully transfected with pHsp 70-HSV-TK and that expression levels of HSV-TK remained stable. Both in vitro and in vivo results indicated that the combination of gene therapy and heat treatment resulted in better therapeutic effects than heating-alone group. The rates of apoptosis and necrosis in the combined treatment group were 49.0% and 7.21%, respectively. The rate of inhibition of cell proliferation in the combined treatment group was significantly higher (87.5%) than that in the heating-alone group (65.8%; Pcombined treatment group were 91.3% and 87.91%, respectively, and were significantly higher than the corresponding rates of the heating-alone group (70.41% and 57.14%; Pcombined treatment group were significantly lower than those in the other groups (Pcombined application of heat treatment and gene therapy has a synergistic and complementary effect and that PEI-MZF-NPs can simultaneously act both as a nonviral gene vector and a magnet-induced source of heat, thereby representing a viable approach for the treatment of cancer.

  15. 33 CFR 165.776 - Security Zone; Coast Guard Base San Juan, San Juan Harbor, Puerto Rico

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Coast Guard Base San Juan, San Juan Harbor, Puerto Rico 165.776 Section 165.776 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED ACCESS AREAS Specific...

  16. Research of Zibu Shenjing Fang on Leptin, Ob-R and NPY level of mice with kidney essence insufficiency%滋补肾精方对肾精不足证小鼠Leptin、Ob-R和NPY表达的影响

    Institute of Scientific and Technical Information of China (English)

    姚军孝; 王玉萍; 沈世林; 何岳珍; 李海; 苏小军; 李立

    2013-01-01

    目的:观察滋补肾精方对肾精不足证小鼠瘦素(Leptin)及瘦素受体(Ob-R)和神经肽Y(NPY)水平的影响,并探讨脾肾相互影响机制.方法:将50只小鼠随机分为正常组、模型组、金匮肾气丸组及滋补肾精方高、低剂量组.造模同时,模型组灌胃0.9%氯化钠溶液(0.02mL/g)、金匮肾气丸组灌胃金匮肾气丸混悬液(2.7g·kg-1·d-1)、滋补肾精方高、低剂量组分别以滋补肾精方(20g·kg-1·d-1、10g·kg-1·d-1)灌胃,连续21d.观察小鼠一般状况,血清Leptin、脑组织Ob-R和NPY表达水平.结果:滋补肾精方能够改善小鼠肾精不足证表现.与模型组比较,滋补肾精方高、低剂量组可明显降低Leptin及Ob-R活性,升高NPY活性(P<0.05,P<0.01).结论:滋补肾精方可能通过调节Leptin及Ob-R与NPY水平的途径发挥补肾填精作用,脾肾相互影响是通过Leptin-Ob-R-NPY轴实现的.%Objective: To observe the effect of Zibu Shenjing Fang on Leptin, Ob-R and NPY level of mice with kidney essence insufficiency, and to explore the each other influence mechanism of spleen and kidney. Methods: 50 healthy male Kunming mouse were divided into 5 groups: normal group, model group, Jinkui Shenqi Wan group, Zibu Shenjing Fang high and low doses group. With established the animal models of kidney essence insufficiency, model group was given saline (0.02mL/g), Jinkui Shenqi Wan group was given Jinkui Shenqi Wan (2.7g·kg-1·d-1), and Zibu Shenjing Fang high-dose and low-dose groups were given Zibu Shenjing Fang (20gkg·d-1,10gkg·d-1) respectively for 21 days. Then observed the general situation of animals, measured the level of Leptin in blood, Ob-R and NPY in Brain tissue. Results: Zibu Shenjing Fang obviously makes the symptoms of the mice with kidney essence insufficiency better, adjusts the activity of Leptin in serum and Ob-R, NPY in Brain tissue (P<0.05, P<0.01). Conclusion: Zibu Shenjing Fang may play an important role of Filling Kidney essence by

  17. Estudo Comparado da Gestão das Visitações nos Parques Estaduais Turísticos do Alto da Ribeira (PETAR e Intervales (PEI, São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Gilberto Sarfati

    2012-04-01

    Full Text Available ResumoO objetivo primário desta pesquisa é investigar porque dois parques estaduais relativamente semelhantes (PETAR e PEI apresentam graus de conservação tão distintos. A hipótese central é que as diferenças podem ser explicadas em função das gestões diferentes, ou seja, o PEI é mais conservado que o PETAR, pois sua gestão é conduzida pela Fundação Florestal com a participação da comunidade local através de uma cooperativa. A análise dos dados e do resultado da pesquisa de campo nos leva a concluir que o PETAR está sofrendo um processo de deterioração de suas cavernas abertas à visitação e de seu entorno fruto do turismo desordenado, configurando assim a incidência da tragédia dos comuns. Já no caso do PEI foi constatada uma visitação mais controlada e limitada e, portanto, um grau de preservação do complexo turístico bastante superior. Conclui-se que o modelo de gestão no qual o PEI é vinculado a Fundação Florestal ajuda a explicar o melhor grau de conservação do parque em relação ao PETAR administrado pelo Instituto Florestal. Palavras-Chave: PETAR; PEI; gestão de visitações; turismo sustentável; tragédia dos comuns.  AbstractThe primary objective of this research is to investigate why

  18. Microbial biogeography of San Francisco Bay sediments

    Science.gov (United States)

    Lee, J. A.; Francis, C. A.

    2014-12-01

    The largest estuary on the west coast of North America, San Francisco Bay is an ecosystem of enormous biodiversity, and also enormous human impact. The benthos has experienced dredging, occupation by invasive species, and over a century of sediment input as a result of hydraulic mining. Although the Bay's great cultural and ecological importance has inspired numerous surveys of the benthic macrofauna, to date there has been almost no investigation of the microbial communities on the Bay floor. An understanding of those microbial communities would contribute significantly to our understanding of both the biogeochemical processes (which are driven by the microbiota) and the physical processes (which contribute to microbial distributions) in the Bay. Here, we present the first broad survey of bacterial and archaeal taxa in the sediments of the San Francisco Bay. We conducted 16S rRNA community sequencing of bacteria and archaea in sediment samples taken bimonthly for one year, from five sites spanning the salinity gradient between Suisun and Central Bay, in order to capture the effect of both spatial and temporal environmental variation on microbial diversity. From the same samples we also conducted deep sequencing of a nitrogen-cycling functional gene, nirS, allowing an assessment of evolutionary diversity at a much finer taxonomic scale within an important and widespread functional group of bacteria. We paired these sequencing projects with extensive geochemical metadata as well as information about macrofaunal distribution. Our data reveal a diversity of distinct biogeographical patterns among different taxa: clades ubiquitous across sites; clades that respond to measurable environmental drivers; and clades that show geographical site-specificity. These community datasets allow us to test the hypothesis that salinity is a major driver of both overall microbial community structure and community structure of the denitrifying bacteria specifically; and to assess

  19. Beneficial Reuse of San Ardo Produced Water

    Energy Technology Data Exchange (ETDEWEB)

    Robert A. Liske

    2006-07-31

    This DOE funded study was performed to evaluate the potential for treatment and beneficial reuse of produced water from the San Ardo oilfield in Monterey County, CA. The potential benefits of a successful full-scale implementation of this project include improvements in oil production efficiency and additional recoverable oil reserves as well as the addition of a new reclaimed water resource. The overall project was conducted in two Phases. Phase I identified and evaluated potential end uses for the treated produced water, established treated water quality objectives, reviewed regulations related to treatment, transport, storage and use of the treated produced water, and investigated various water treatment technology options. Phase II involved the construction and operation of a small-scale water treatment pilot facility to evaluate the process's performance on produced water from the San Ardo oilfield. Cost estimates for a potential full-scale facility were also developed. Potential end uses identified for the treated water include (1) agricultural use near the oilfield, (2) use by Monterey County Water Resources Agency (MCWRA) for the Salinas Valley Water Project or Castroville Seawater Intrusion Project, (3) industrial or power plant use in King City, and (4) use for wetlands creation in the Salinas Basin. All of these uses were found to have major obstacles that prevent full-scale implementation. An additional option for potential reuse of the treated produced water was subsequently identified. That option involves using the treated produced water to recharge groundwater in the vicinity of the oil field. The recharge option may avoid the limitations that the other reuse options face. The water treatment pilot process utilized: (1) warm precipitation softening to remove hardness and silica, (2) evaporative cooling to meet downstream temperature limitations and facilitate removal of ammonia, and (3) reverse osmosis (RO) for removal of dissolved salts, boron

  20. SAN/CXFS test report to LLNL

    Energy Technology Data Exchange (ETDEWEB)

    Ruwart, T M; Eldel, A

    2000-01-01

    The primary objectives of this project were to evaluate the performance of the SGI CXFS File System in a Storage Area Network (SAN) and compare/contrast it to the performance of a locally attached XFS file system on the same computer and storage subsystems. The University of Minnesota participants were asked to verify that the performance of the SAN/CXFS configuration did not fall below 85% of the performance of the XFS local configuration. There were two basic hardware test configurations constructed from the following equipment: Two Onyx 2 computer systems each with two Qlogic-based Fibre Channel/XIO Host Bus Adapter (HBA); One 8-Port Brocade Silkworm 2400 Fibre Channel Switch; and Four Ciprico RF7000 RAID Disk Arrays populated Seagate Barracuda 50GB disk drives. The Operating System on each of the ONYX 2 computer systems was IRIX 6.5.6. The first hardware configuration consisted of directly connecting the Ciprico arrays to the Qlogic controllers without the Brocade switch. The purpose for this configuration was to establish baseline performance data on the Qlogic controllers / Ciprico disk raw subsystem. This baseline performance data would then be used to demonstrate any performance differences arising from the addition of the Brocade Fibre Channel Switch. Furthermore, the performance of the Qlogic controllers could be compared to that of the older, Adaptec-based XIO dual-channel Fibre Channel adapters previously used on these systems. It should be noted that only raw device tests were performed on this configuration. No file system testing was performed on this configuration. The second hardware configuration introduced the Brocade Fibre Channel Switch. Two FC ports from each of the ONYX2 computer systems were attached to four ports of the switch and the four Ciprico arrays were attached to the remaining four. Raw disk subsystem tests were performed on the SAN configuration in order to demonstrate the performance differences between the direct-connect and the

  1. 太白山自然保护区金背杜鹃菌根调查研究%A Study on Rhododendron clementiae Forrest ex W.W. Smilh Subsp.aureodorsale W.P.Fang' Mycorrhizae in the Nature Preserve of the Taibai Mountains

    Institute of Scientific and Technical Information of China (English)

    吴重华; 王吉忍; 杨俊秀; 胡崇德

    2000-01-01

    首次报道了在太白山自然保护区巴山冷杉林下采到的金背杜鹃(Rhododendron clementinae Forrest ex W.W. Smith subsp. aureodorsale W. P. Fang)共生真菌3种:卷缘网褶菌(Poxillus involotes (Botsch) Fr.)、亚褐环粘盖牛肝(Suillus subluteus (Peck) Snell ex Slipp & Snell.)、黄粉牛肝菌(Pulveroboletus ravenelii Berk. et Curt.),且这3种与金背杜鹃形成外生菌根.

  2. La depuradora de Begues : reactor biològic o aiguamolls construïts?. Estudi comparatiu dels vectors : paràmetres de l'H2O, fangs produïts, costos capitals i d'explotació, personal

    OpenAIRE

    Gómez Castillo, Jordi

    2010-01-01

    L'objectiu del present estudi és comparar els vectors de paràmetres de l'aigua, producció de fangs, costos i personal entre 2 tipus d'instal·lacions EDAR al municipi de Begues; una ja existent amb tractament secundari i terciari mitjançant un reactor biològic i una potencial amb tractament secundari i terciari mitjançant aiguamolls construïts. La finalitat del projecte és determinar, gràcies a l'estudi dels principals vectors ambientals de la infraestructura i a altres estudiats per na Susana...

  3. Reflections on Mr. Fang Guoyu’s Research Methodology Embodied in Textual Criticisms and Explanations of Historical Geography of Southwestern China%关于《中国西南历史地理考释》研究方法的再思考

    Institute of Scientific and Technical Information of China (English)

    谭世圆

    2016-01-01

    Mr. Fang Guoyu,a famous historian,made groundbreaking contribution to the research on the his-tory and geography of China’s southwestern borders. His methodology for textual criticisms and explanation of his-torical geography is represented in Textual Criticisms and Explanations of Historical Geography of Southwestern China. Mr. Fang emphasizes the observance and comparison of literatures and applies the methods in the study of historical sources to the analysis of historical materials collected. Although great importance is attached to the transmission of historical sources and materials,Mr. Fang does not restrict his research to these historical materi-als. In close association with historical reality,Mr. Fang employs the Marxist approach of historical materialism to interpret factual historical activities in terms of different regional characteristics in different periods of history and to make textual criticisms and explanations of toponyms in terms of the evolution of geographical conditions and ad-ministrative divisions.%方国瑜先生是著名的历史学家,在西南边疆史地研究方面有开创性的贡献。从《中国西南历史地理考释》中可总结其历史地理考释的方法。方先生重视文献的参稽考索,将史源学方法运用于所搜集的史料,重视史源和史料流传,但又不局限于史料。他紧密结合历史实际,运用马克思主义历史唯物主义的方法,从每一个时期、每一个地区的具体情况来阐释具体历史活动,根据地理环境、政治区划的演变来考释地名。

  4. Research on influence and mechanism of Roujin Fang on jaw-opening reflex of tension-type headache rats%柔筋方对紧张型头痛大鼠张颌反射的影响及机制研究

    Institute of Scientific and Technical Information of China (English)

    吴宏赟; 胡志强

    2012-01-01

    目的 探索柔筋方对紧张型头痛大鼠张颌反射的影响及作用机制.方法 采用颈部双侧半棘肌局部注射三磷酸腺苷二钠注射液(ATP)制造紧张型头痛的动物实验模型.体重(254±28)g 的SPF 级Wistar 大鼠40 只,分为假手术组、模型组、柔筋方组和一氧化氮合成酶(NOS)抑制剂组,通过张颌反射定量分析紧张型头痛模型大鼠头区皮肤肌肉筋膜疼痛阈值及敏感性的变化.结果 柔筋方组和NOS 抑制剂组潜伏期延长(P < 0.05 或P < 0.01),模型组疼痛阈值降低(P < 0.01),柔筋方组和NOS 抑制剂组疼痛阈值有下降趋势,但差异无统计学意义(P > 0.05).结论 柔筋方可显著延长潜伏期,并减低疼痛阈值,降低头颈部肌筋膜敏感性,疗效机制与降低一氧化氮浓度有关.%Objective To study influence and mechanism of Roujin Fang on jaw-opening reflex of tension-type headache rats. Methods Models were established by injecting adenosine disodium triphosphate injection (ATP) in both semispinalis neck intramuscular. 40 Wistar SPF rats weighed (254±28) g were selected, and randomly divided into sham-operated group, model group, Roujin Fang group and nitric oxide synthase (NOS) group. Accroding to jaw-opening reflex (JOR), myofascial pain threshold and sensitivity were quantitatively measured. Results Latency in Roujin Fang group and NOS group were significantly longer than that in pre-injection (P 0.05). Conclusion Roujin Fang can remarkably prolong latency, decrease pain threshold and reduce myofascial sensitivity, the mecha -nism is related to consistency of nitric oxide.

  5. Amplification sans bruit d'images optiques

    Science.gov (United States)

    Gigan, S.; Delaubert, V.; Lopez, L.; Treps, N.; Maitre, A.; Fabre, C.

    2004-11-01

    Nous utilisons un Oscillateur Paramétrique Optique (OPO) pompé sous le seuil dans le but d'amplifier une image multimode transverse sans dégradation du rapport signal à bruit. Le dispositif expérimental met en œuvre un OPO de type II triplement résonant et semi-confocal pour le faisceau amplifié. L'existence d'effets quantiques lors de l'amplification multimode dans un tel dispositif a été montrée expérimentalement. Plus généralement, ceci nous a amené à étudier les propriétés quantiques transverses des faisceaux lumineux amplifiés. Une telle étude peut trouver des applications non seulement en imagerie, mais également dans le traitement quantique de l'information.

  6. San Carlos Apache Tribe - Energy Organizational Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Rapp, James; Albert, Steve

    2012-04-01

    The San Carlos Apache Tribe (SCAT) was awarded $164,000 in late-2011 by the U.S. Department of Energy (U.S. DOE) Tribal Energy Program's "First Steps Toward Developing Renewable Energy and Energy Efficiency on Tribal Lands" Grant Program. This grant funded:  The analysis and selection of preferred form(s) of tribal energy organization (this Energy Organization Analysis, hereinafter referred to as "EOA").  Start-up staffing and other costs associated with the Phase 1 SCAT energy organization.  An intern program.  Staff training.  Tribal outreach and workshops regarding the new organization and SCAT energy programs and projects, including two annual tribal energy summits (2011 and 2012). This report documents the analysis and selection of preferred form(s) of a tribal energy organization.

  7. Stratigraphy, sedimentology and paleontology of lower Eocene San Jose formation, central San Juan basin, New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Lucas, S.G.; Smith, L.N. (New Mexico Museum of Natural History, Albuquerque (USA))

    1989-09-01

    The lower Eocene San Jose Formation in the central portion of the San Juan basin (Gobernador-Vigas Canyon area) consists of the Cuba Mesa, Regina, Llaves, and Tapicitos Members. Well log data indicate that, from its 100-m thickness, the Cuba Mesa Member thins toward the basin center and pinches out to the northeast by lat. 36{degree}40'N, long. 107{degree}19'W. The Regina Member has the most extensive outcrops in the central basin, and it decreases in sandstone/mud rock ratio to the north. The Llaves and Tapicitos Members occur only at the highest elevations, are thin due to erosion, and are not mappable as separate units. Well log data and 1,275 m of measured stratigraphic section in the Regina, Llaves, and Tapicitos Members indicate these strata are composed of approximately 35% medium to coarse-grained sandstone and 65% fine-grained sandstone and mud rock. Sedimentology and sediment-dispersal patterns indicate deposition by generally south-flowing streams that had sources to the northwest, northeast, and east. Low-sinuosity, sand-bedded, braided( ) streams shifted laterally across about 1 km-wide channel belts to produce sheet sandstones that are prominent throughout the San Jose Formation. Subtle levees separated channel environments from floodplain and local lacustrine areas. Avulsion relocated channels periodically to areas on the floodplain, resulting in the typically disconnected sheet sandstones within muddy overbank deposits of the Regina Member.

  8. Puente Coronado - San Diego (EE. UU.

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1971-12-01

    Full Text Available This 3,5 km long bridge, joining the cities of San Diego and Coronado is one of the longest in the world of this type, and one of the three most important straight line bridges in the United States. Its supporting structure consists of reinforced concrete columns resting on footings or piles, according to whether they are under the sea water or on dry land. The superstructure is partly of metal plates and partly of box girders. The surfacing of the deck consists of asphalt epoxy concrete, of 5 cm depth. Special paint was applied to the bridge, including layers of vinyl, iron oxide and blue vinyl on a zinc base.Este puente, de unos 3 km y medio, que une las ciudades de San Diego y Coronado es uno de los de mayor longitud del mundo, de este tipo, y uno de los tres principales ortótropos de los Estados Unidos de América. Su infraestructura está constituida por pilas de hormigón armado apoyadas sobre pilotes o sobre zapatas, según estén en el mar o en tierra firme. La superestructura está formada, en parte, por chapas metálicas y, en parte, por vigas cajón. El acabado del tablero metálico se realizó a base de hormigón asfáltico de epoxi con un espesor de 5 cm. La pintura es especial y se compone de capas de vinilo, de óxido de hierro y de vinilo azul sobre una capa de cinc.

  9. Paleohydrogeology of the San Joaquin basin, California

    Science.gov (United States)

    Wilson, A.M.; Garven, G.; Boles, J.R.

    1999-01-01

    Mass transport can have a significant effect on chemical diagenetic processes in sedimentary basins. This paper presents results from the first part of a study that was designed to explore the role of an evolving hydrodynamic system in driving mass transport and chemical diagenesis, using the San Joaquin basin of California as a field area. We use coupled hydrogeologic models to establish the paleohydrogeology, thermal history, and behavior of nonreactive solutes in the basin. These models rely on extensive geological information and account for variable-density fluid flow, heat transport, solute transport, tectonic uplift, sediment compaction, and clay dehydration. In our numerical simulations, tectonic uplift and ocean regression led to large-scale changes in fluid flow and composition by strengthening topography-driven fluid flow and allowing deep influx of fresh ground water in the San Joaquin basin. Sediment compaction due to rapid deposition created moderate overpressures, leading to upward flow from depth. The unusual distribution of salinity in the basin reflects influx of fresh ground water to depths of as much as 2 km and dilution of saline fluids by dehydration reactions at depths greater than ???2.5 km. Simulations projecting the future salinity of the basin show marine salinities persisting for more than 10 m.y. after ocean regression. Results also show a change from topography-to compaction-driven flow in the Stevens Sandstone at ca. 5 Ma that coincides with an observed change in the diagenetic sequence. Results of this investigation provide a framework for future hydrologic research exploring the link between fluid flow and diagenesis.

  10. Lahar-hazard zonation for San Miguel volcano, El Salvador

    Science.gov (United States)

    Major, J.J.; Schilling, S.P.; Pullinger, C.R.; Escobar, C.D.; Chesner, C.A.; Howell, M.M.

    2001-01-01

    San Miguel volcano, also known as Chaparrastique, is one of many volcanoes along the volcanic arc in El Salvador. The volcano, located in the eastern part of the country, rises to an altitude of about 2130 meters and towers above the communities of San Miguel, El Transito, San Rafael Oriente, and San Jorge. In addition to the larger communities that surround the volcano, several smaller communities and coffee plantations are located on or around the flanks of the volcano, and the PanAmerican and coastal highways cross the lowermost northern and southern flanks of the volcano. The population density around San Miguel volcano coupled with the proximity of major transportation routes increases the risk that even small volcano-related events, like landslides or eruptions, may have significant impact on people and infrastructure. San Miguel volcano is one of the most active volcanoes in El Salvador; it has erupted at least 29 times since 1699. Historical eruptions of the volcano consisted mainly of relatively quiescent emplacement of lava flows or minor explosions that generated modest tephra falls (erupted fragments of microscopic ash to meter sized blocks that are dispersed into the atmosphere and fall to the ground). Little is known, however, about prehistoric eruptions of the volcano. Chemical analyses of prehistoric lava flows and thin tephra falls from San Miguel volcano indicate that the volcano is composed dominantly of basalt (rock having silica content

  11. 李恒《袖珍方》明刊本流布情况考%Textual research on circulation of the Ming edition of Li Heng’ s Xiu zhen fang (Pocket Formulary)

    Institute of Scientific and Technical Information of China (English)

    杨金萍; 刘鹏; 路明静; 卢星; 李绍林; 金秀梅

    2015-01-01

    《袖珍方》是在明代朱橚主持及授意下,由周王府良医所的李恒编撰的一部方书。该书曾于洪武、永乐年间编刊过2次,因实用性强,后世有多种版本流传,仅明代就刊刻过多次。目前所见最早刊本为永乐年间刊小字本,明正德四年刊本是在永乐本基础上的重刊本,属于同一版本系统;明正统年间以后出现多种以“魁本”“大全”命名的刊本,多是坊刻本,与永乐本系统相比,不论版式还是内容有较大的差异。%Xiu zhen fang ( Pocket Formulary) is a recipe book of the Ming Dynasty, inspired and managed by Zhu Su, compiled by Li Heng of liangyisuo ( good physician house) in Zhou wangfu ( Zhou’ s royal palace) .The book was compiled and published twice during the reigns of the Hongwu and Yongle Em-perors of the Ming Dynasty.Because of its high practicability, there were some editions in circulation, and the book was published several times only in the Ming Dynasty.At present, the earliest extanteditionwas the little character version of Yongle, and the version in the 4th year of Zhengde Emperor of the Ming Dynasty was a reprinting edition based on the Yongle edition, sharingthe same edition system.Most of the editions appeared after the reign of Zhengtong Emperor of the Ming Dynasty, titled by“kui ben( head version)”and“da quan( complete edition)”were the editionspublished in the local bookshops, which had rather distinct differences from the Yongle edition system not only in the its format but also in its contents.

  12. 固肾培元方治疗老年性聋临床观察%Clinical Observation on the Therapeutic Effect of Gushen Peiyuan Fang in Treating 35 Patients of Presbycusis

    Institute of Scientific and Technical Information of China (English)

    李莉; 李卫华; 毋桂花

    2012-01-01

    Objective; To observe the therapeutic effect of Gushen Peiyuan Fang (JPF ) on Presbycusis based on a clinical trial. Methods: Sixty-seven cases with Presbycusis were randomly divided into two groups equally,i.e. controlling groups( CG Were treated by Golden theragpam and Tanakan Tablets,treating group( TG ) treated by JPF based on the Golden theragpam and Tanakan Tablets.Then, evaluations on the therapeutic effect were carried out respectively following the end of the therapies. Results: The total effective rate was 62.9% among the cases of TG,while it was 53.1% among those of CG,with no statistical significance( P>0.05 ) between them. But the TG was higher than the CG. Conclusions: JPF in treating Presbycusis in the total efficiency is better than that of control group, Chinese medicine may improve the inner ear microcirculation, promote functional recovery of hair cells, thereby enhancing the hearing.%目的:观察固肾培元方治疗老年性聋的临床疗效.方法:67例老年性聋患者,随机分为两组.对照组口服金施尔康和达纳康,治疗组在口服金施尔康和达纳康的基础上,给予固肾培元方治疗,用药结束后进行疗效观察.结果:治疗组总有效率62.9%,对照组总有效率53.1%.两组总有效率比较无显著性意义(P>0.05),但治疗组总有效率高于对照组.结论:固肾培元方治疗老年性耳聋总有效率优于对照组,提示补肾活血通窍中药可能通过改善内耳局部微循环,促进内耳毛细胞功能恢复,从而起到提高听力的作用.

  13. Rational Thinking on Chen Shuwei Appeal-A Discussion with Mr.Fang Kong%对陈书伟上诉案的理性思考--兼与方孔先生商榷

    Institute of Scientific and Technical Information of China (English)

    聂帅钧

    2015-01-01

    “操”字状一经报道便引起激烈的讨论,而拘留决定的作出更将此事推向了高潮。方孔先生从人的理性和法律的理性出发,指出“操”字状并未侮辱司法工作人员及法律,但其观点过于理想化且未考虑我国国情。“操”字状中折射出的言论自由、社会常理、司法权威等法治问题引人深思,笔者认为言论自由应存在一定的界限,理性的法律应包含社会常理,司法权威应得到进一步加强。总之,反思该案将为建设社会主义法治国家提供不少经验和启示。%The report on the case of “Cao”character caused a heated discussion,and the detention made this case to a climax.In perspective of human rationality and reason,Mr.Fang Kong pointed out that the case of“Cao”character in petition for appeal that doesn’t insult the judicial clerks and laws,however,the view is so idealistic without considering the national conditions of our country.The case of “Cao”character reflects some problems such as the freedom of speech,social convention,and judicial authority.It is believed that freedom of speech should be limited in certain boundaries,the rationality of the law should include the conventions of the society,and the judicial authority should be further strengthened.In a word,the rethinking will provide some experiences and enlightenments for construction of socialist China ruled by law.

  14. SANS spectra of the fractal supernucleosomal chromatin structure models

    Science.gov (United States)

    Ilatovskiy, Andrey V.; Lebedev, Dmitry V.; Filatov, Michael V.; Petukhov, Michael G.; Isaev-Ivanov, Vladimir V.

    2012-03-01

    The eukaryotic genome consists of chromatin—a nucleoprotein complex with hierarchical architecture based on nucleosomes, the organization of higher-order chromatin structures still remains unknown. Available experimental data, including SANS spectra we had obtained for whole nuclei, suggested fractal nature of chromatin. Previously we had built random-walk supernucleosomal models (up to 106 nucleosomes) to interpret our SANS spectra. Here we report a new method to build fractal supernucleosomal structure of a given fractal dimension or two different dimensions. Agreement between calculated and experimental SANS spectra was significantly improved, especially for model with two fractal dimensions—3 and 2.

  15. San Pascual (2008) Año XLVI, n. 347

    OpenAIRE

    Pérez, María Dolores, O.S.C. (Directora)

    2008-01-01

    Editorial. Restauración de esta obra de arte. Homilía del Excmo. y rvdmo. Sr. Obispo de la diócesis Dr. D. Casimirio López Llorente. Siguiendo los caminos de San Pascual. Entrevista D. Francisco José Cortes Blasco, cura, párroco de la Iglesia arciprestal de nuestra ciudad. La importancia de la eucaristía. Vida en el santuario. Rincón poético. La pila del bautismo de la parroquia de San Jaime de vila-real. El corazón de un Santo (IV). Milagros de San Pascual IX. Confidencias. ¡Alabado seas, se...

  16. 40 CFR 81.164 - San Diego Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false San Diego Intrastate Air Quality... Quality Control Regions § 81.164 San Diego Intrastate Air Quality Control Region. The San Diego Intrastate... within the outermost boundaries of the area so delimited): In the State of California: San Diego County. ...

  17. 77 FR 43350 - Draft Environmental Assessment and Proposed Habitat Conservation Plan for the San Diego Unified...

    Science.gov (United States)

    2012-07-24

    ... San Diego Unified School District's Jonas Salk Elementary School Project in the City of San Diego, San Diego County, CA AGENCY: Fish and Wildlife Service, Interior. ACTION: Notice of availability: request... Elementary School Project in response to an application from the San Diego Unified School District (District...

  18. The San Andreas fault in the San Francisco Bay region, California: Structure and kinematics of a Young plate boundary

    Science.gov (United States)

    Jachens, R.C.; Zoback, M.L.

    1999-01-01

    Recently acquired high-resolution aeromagnetic data delineate offset and/or truncated magnetic rock bodies of the Franciscan Complex that define the location and structure of, and total offset across, the San Andreas fault in the San Francisco Bay region. Two distinctive magnetic anomalies caused by ultramafic rocks and metabasalts east of, and truncated at, the San Andreas fault have clear counterparts west of the fault that indicate a total right-lateral offset of only 22 km on the Peninsula segment, the active strand that ruptured in 1906. The location of the Peninsula segment is well defined magnetically on the northern peninsula where it goes offshore, and can be traced along strike an additional ~6 km to the northwest. Just offshore from Lake Merced, the inferred fault trace steps right (northeast) 3 km onto a nearly parallel strand that can be traced magnetically northwest more than 20 km as the linear northeast edge of a magnetic block bounded by the San Andreas fault, the Pilarcitos fault, and the San Gregorio-Hosgri fault zone. This right-stepping strand, the Golden Gate segment, joins the eastern mapped trace of the San Andreas fault at Bolinas Lagoon and projects back onshore to the southeast near Lake Merced. Inversion of detailed gravity data on the San Francisco Peninsula reveals a 3 km wide basin situated between the two strands of the San Andreas fault, floored by Franciscan basement and filled with Plio-Quaternary sedimentary deposits of the Merced and Colma formations. The basin, ~1 km deep at the coast, narrows and becomes thinner to the southeast along the fault over a distance of ~12 km. The length, width, and location of the basin between the two strands are consistent with a pull-apart basin formed behind the right step in the right-lateral strike-slip San Andreas fault system and currently moving southeast with the North American plate. Slight nonparallelism of the two strands bounding the basin (implying a small component of convergence

  19. Forecasting Selenium Discharges to the San Francisco Bay-Delta Estuary: Ecological Effects of A Proposed San Luis Drain Extension

    Science.gov (United States)

    Presser, Theresa S.; Luoma, Samuel N.

    2006-01-01

    Selenium discharges to the San Francisco Bay-Delta Estuary (Bay-Delta) could change significantly if federal and state agencies (1) approve an extension of the San Luis Drain to convey agricultural drainage from the western San Joaquin Valley to the North Bay (Suisun Bay, Carquinez Strait, and San Pablo Bay); (2) allow changes in flow patterns of the lower San Joaquin River and Bay-Delta while using an existing portion of the San Luis Drain to convey agricultural drainage to a tributary of the San Joaquin River; or (3) revise selenium criteria for the protection of aquatic life or issue criteria for the protection of wildlife. Understanding the biotransfer of selenium is essential to evaluating effects of selenium on Bay-Delta ecosystems. Confusion about selenium threats to fish and wildlife stem from (1) monitoring programs that do not address specific protocols necessary for an element that bioaccumulates; and (2) failure to consider the full complexity of the processes that result in selenium toxicity. Past studies show that predators are more at risk from selenium contamination than their prey, making it difficult to use traditional methods to predict risk from environmental concentrations alone. This report presents an approach to conceptualize and model the fate and effects of selenium under various load scenarios from the San Joaquin Valley. For each potential load, progressive forecasts show resulting (1) water-column concentration; (2) speciation; (3) transformation to particulate form; (4) particulate concentration; (5) bioaccumulation by invertebrates; (6) trophic transfer to predators; and (7) effects on those predators. Enough is known to establish a first-order understanding of relevant conditions, biological response, and ecological risks should selenium be discharged directly into the North Bay through a conveyance such as a proposed extension of the San Luis Drain. The approach presented here, the Bay-Delta selenium model, determines the mass, fate

  20. San Diego Littoral Cell CRSMP Potential Offshore Borrow Areas 2009

    Data.gov (United States)

    California Department of Resources — Offshore sediment sources along the entire reach of the San Diego Coastal RSM Plan region were previously identified by SANDAG and used for Regional Beach Sand...

  1. Port San Luis, California Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Port San Luis, California Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST)...

  2. Marine Seismic Reflection Profiles Data of San Francisco Bay Area

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Dataset consists of two-dimensional marine seismic reflection profile data from the San Francisco Bay area. These data were acquired in 1995, with the vessel Robert...

  3. Vernal Pool Amphibians, Shrimp, Plants - San Diego [ds188

    Data.gov (United States)

    California Department of Resources — In 2002, the City of San Diego (City) received funding through a U. S. Fish and Wildlife Service (USFWS) Section 6 Planning Grant to complete an inventory and...

  4. San Joaquin River National Wildlife Refuge: Final Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on San Joaquin River NWR for the next 15 years. This plan outlines the Refuge vision and...

  5. Folds--Offshore of San Francisco Map Area, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for folds for the geologic and geomorphic map of the Offshore of San Francisco map area, California. The vector data file is...

  6. Faults--Offshore of San Francisco Map Area, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for faults for the geologic and geomorphic map of the Offshore San Francisco map area, California. The vector data file is included...

  7. Faults--Offshore of San Gregorio Map Area, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the faults for the geologic and geomorphic map (see sheet 10, SIM 3306) of the Offshore of San Gregorio map area, California....

  8. Final San Andres National Wildlife Refuge Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on San Andres NWR for the next 15 years. This plan outlines the Refuge vision and purpose...

  9. San Francisco Littoral Cell CRSMP Sensitive Habitat 2012

    Data.gov (United States)

    California Department of Resources — Ecological regions of interest as identified in the San Francisco Coastal Region Sediment Management Plan.NPS identifies several special management areas within the...

  10. Folds--Offshore of San Gregorio Map Area, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the folds for the geologic and geomorphic map (see sheet 10, SIM 3306) of the Offshore of San Gregorio map area, California....

  11. Faults--Offshore of San Francisco Map Area, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for faults for the geologic and geomorphic map of the Offshore San Francisco map area, California. The vector data file is included...

  12. Folds--Offshore of San Francisco Map Area, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for folds for the geologic and geomorphic map of the Offshore of San Francisco map area, California. The vector data file is...

  13. San Francisco Littoral Cell CRSMP Sensitive Habitat 2012

    Data.gov (United States)

    California Department of Resources — Ecological regions of interest as identified in the San Francisco Coastal Region Sediment Management Plan.NPS identifies several special management areas within the...

  14. Narrative Report : San Francisco Bay NWR Complex : Fiscal Year 1974

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual narrative report details the conditions and management of San Francisco Bay National Wildlife Refuge in 1974. The report begins with a general...

  15. Backscatter C [8101]--Offshore of San Francisco, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of DS 781 presents data for the acoustic-backscatter map of the Offshore of San Francisco map area, California. Backscatter data are provided as separate...

  16. San Francisco Bay National Wildlife Refuge Complex : Annual Narrative 1980

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual narrative report details the conditions and management of San Francisco Bay National Wildlife Refuge in 1980. The report begins with a general...

  17. San Pablo Bay National Wildlife Refuge: Final Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on San Pablo Bay NWR for the next 15 years. This plan outlines the Refuge vision and...

  18. Faults--Offshore of San Gregorio Map Area, California

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This part of SIM 3306 presents data for the faults for the geologic and geomorphic map (see sheet 10, SIM 3306) of the Offshore of San Gregorio map area, California....

  19. Avian botulism in the southern San Joaquin valley 1970

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A joint effort of the Department of Fish and' Game and the U. S. Bureau of Sport Fisheries and Wildlife contained botulism losses in the southern San Joaquin Valley...

  20. Contaminants investigation of the San Antonio River of Texas, 1995

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — In 1992, the U.S. Fish and Wildlife Service conducted a contaminants investigation on fish collected from the San Antonio and Guadalupe Rivers in southeast Texas....