Differential diagnosis of feline leukemia virus subgroups using pseudotype viruses expressing green fluorescent protein.

Science.gov (United States)

Nakamura, Megumi; Sato, Eiji; Miura, Tomoyuki; Baba, Kenji; Shimoda, Tetsuya; Miyazawa, Takayuki

2010-06-01

Feline leukemia virus (FeLV) is classified into three receptor interference subgroups, A, B and C. In this study, to differentiate FeLV subgroups, we developed a simple assay system using pseudotype viruses expressing green fluorescent protein (GFP). We prepared gfp pseudotype viruses, named gfp(FeLV-A), gfp(FeLV-B) and gfp(FeLV-C) harboring envelopes of FeLV-A, B and C, respectively. The gfp pseudotype viruses completely interfered with the same subgroups of FeLV reference strains on FEA cells (a feline embryonic fibroblast cell line). We also confirmed that the pseudotype viruses could differentiate FeLV subgroups in field isolates. The assay will be useful for differential diagnosis of FeLV subgroups in veterinary diagnostic laboratories in the future.

Predictive modelling of Ross River virus notifications in southeastern Australia.

Science.gov (United States)

Cutcher, Z; Williamson, E; Lynch, S E; Rowe, S; Clothier, H J; Firestone, S M

2017-02-01

Ross River virus (RRV) is a mosquito-borne virus endemic to Australia. The disease, marked by arthritis, myalgia and rash, has a complex epidemiology involving several mosquito species and wildlife reservoirs. Outbreak years coincide with climatic conditions conducive to mosquito population growth. We developed regression models for human RRV notifications in the Mildura Local Government Area, Victoria, Australia with the objective of increasing understanding of the relationships in this complex system, providing trigger points for intervention and developing a forecast model. Surveillance, climatic, environmental and entomological data for the period July 2000-June 2011 were used for model training then forecasts were validated for July 2011-June 2015. Rainfall and vapour pressure were the key factors for forecasting RRV notifications. Validation of models showed they predicted RRV counts with an accuracy of 81%. Two major RRV mosquito vectors (Culex annulirostris and Aedes camptorhynchus) were important in the final estimation model at proximal lags. The findings of this analysis advance understanding of the drivers of RRV in temperate climatic zones and the models will inform public health agencies of periods of increased risk.

Determinants of foamy virus envelope glycoprotein mediated resistance to superinfection

International Nuclear Information System (INIS)

Berg, Angelika; Pietschmann, Thomas; Rethwilm, Axel; Lindemann, Dirk

2003-01-01

Little is known about the nature of foamy virus (FV) receptor molecules on target cells and their interaction with the viral glycoproteins. Similar to other viruses, cellular expression of the FV Env protein is sufficient to induce resistance to exogenous FV, a phenomenon called superinfection resistance (SIR). In this study we define determinants of the FV Env protein essential for mediating SIR. FV Env requires the extracellular domains of the SU and the TM subunits as well as membrane anchorage, efficient cell surface transport, and most probably correct subunit processing. This is in contrast to murine leukemia virus where secreted proteins comprising the receptor-binding domain in SU are sufficient to induce SIR. Furthermore, we demonstrate that cellular expression of the prototype FV envelope proteins induces SIR against pseudotypes with glycoproteins of other FV species, including of simian, feline, bovine, and equine origin. This implies that all of them use the same receptor molecules for viral entry

Vesicular stomatitis virus expressing a chimeric Sindbis glycoprotein containing an Fc antibody binding domain targets to Her2/neu overexpressing breast cancer cells

International Nuclear Information System (INIS)

Bergman, Ira; Whitaker-Dowling, Patricia; Gao Yanhua; Griffin, Judith A.; Watkins, Simon C.

2003-01-01

Vesicular stomatitis virus (VSV) is a candidate for development for cancer therapy. It is an oncolytic virus that is safe in humans. Recombinant virus can be made directly from plasmid components. We attempted to create a virus that targeted specifically to breast cancer cells. Nonreplicating and replicating pseudotype VSV were created whose only surface glycoprotein (gp) was a Sindbis gp, called Sindbis-ZZ, modified to severely reduce its native binding function and to contain the Fc-binding domain of Staphylococcus aureus protein A. When titered on Her2/neu overexpressing SKBR3 human breast cancer cells, pseudotype VSV coated with Sindbis-ZZ had 5 /ml. This work demonstrates the ability to easily create, directly from plasmid components, an oncolytic replicating VSV with a restricted host cell range

Efficient transduction of equine adipose-derived mesenchymal stem cells by VSV-G pseudotyped lentiviral vectors.

Science.gov (United States)

Petersen, Gayle F; Hilbert, Bryan; Trope, Gareth; Kalle, Wouter; Strappe, Padraig

2014-12-01

Equine adipose-derived mesenchymal stem cells (EADMSC) provide a unique cell-based approach for treatment of a variety of equine musculoskeletal injuries, via regeneration of diseased or damaged tissue, or the secretion of immunomodulatory molecules. These capabilities can be further enhanced by genetic modification using lentiviral vectors, which provide a safe and efficient method of gene delivery. We investigated the suitability of lentiviral vector technology for gene delivery into EADMSC, using GFP expressing lentiviral vectors pseudotyped with the G glycoprotein from the vesicular stomatitis virus (V-GFP) or, for the first time, the baculovirus gp64 envelope protein (G-GFP). In this study, we produced similarly high titre V-GFP and G-GFP lentiviral vectors. Flow cytometric analysis showed efficient transduction using V-GFP; however G-GFP exhibited a poor ability to transduce EADMSC. Transduction resulted in sustained GFP expression over four passages, with minimal effects on cell viability and doubling time, and an unaltered chondrogenic differentiation potential. Copyright © 2014 Elsevier Ltd. All rights reserved.

Isolation and characterization of broadly neutralizing human monoclonal antibodies to the e1 glycoprotein of hepatitis C virus

DEFF Research Database (Denmark)

Meunier, Jean-Christophe; Russell, Rodney S.; Goossens, Vera

2008-01-01

The relative importance of humoral and cellular immunity in the prevention or clearance of hepatitis C virus (HCV) infection is poorly understood. However, there is considerable evidence that neutralizing antibodies are involved in disease control. Here we describe the detailed analysis of human...... monoclonal antibodies (MAbs) directed against HCV glycoprotein E1, which may have the potential to control HCV infection. We have identified two MAbs that can strongly neutralize HCV-pseudotyped particles (HCVpp) bearing the envelope glycoproteins of genotypes 1a, 1b, 4a, 5a, and 6a and less strongly...... neutralize HCVpp bearing the envelope glycoproteins of genotype 2a. Genotype 3a was not neutralized. The epitopes for both MAbs were mapped to the region encompassing amino acids 313 to 327. In addition, robust neutralization was also observed against cell culture-adapted viruses of genotypes 1a and 2a...

Victoria Land, Ross Sea, and Ross Ice Shelf, Antarctica

Science.gov (United States)

2002-01-01

On December 19, 2001, MODIS acquired data that produced this image of Antarctica's Victoria Land, Ross Ice Shelf, and the Ross Sea. The coastline that runs up and down along the left side of the image denotes where Victoria Land (left) meets the Ross Ice Shelf (right). The Ross Ice Shelf is the world's largest floating body of ice, approximately the same size as France. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

Epidemiologic patterns of Ross River virus disease in Queensland, Australia, 2001-2011.

Science.gov (United States)

Yu, Weiwei; Mengersen, Kerrie; Dale, Pat; Mackenzie, John S; Toloo, Ghasem Sam; Wang, Xiaoyu; Tong, Shilu

2014-07-01

Ross River virus (RRV) infection is a debilitating disease that has a significant impact on population health, economic productivity, and tourism in Australia. This study examined epidemiologic patterns of RRV disease in Queensland, Australia, during January 2001-December 2011 at a statistical local area level. Spatio-temporal analyses were used to identify the patterns of the disease distribution over time stratified by age, sex, and space. The results show that the mean annual incidence was 54 per 100,000 persons, with a male:female ratio of 1:1.1. Two space-time clusters were identified: the areas adjacent to Townsville, on the eastern coast of Queensland, and the southeast areas. Thus, although public health intervention should be considered across all areas in which RRV occurs, it should specifically focus on high-risk regions, particularly during summer and autumn to reduce the social and economic impacts of RRV infection. © The American Society of Tropical Medicine and Hygiene.

Epidemiologic Patterns of Ross River Virus Disease in Queensland, Australia, 2001–2011

Science.gov (United States)

Yu, Weiwei; Mengersen, Kerrie; Dale, Pat; Mackenzie, John S.; Toloo, Ghasem (Sam); Wang, Xiaoyu; Tong, Shilu

2014-01-01

Ross River virus (RRV) infection is a debilitating disease that has a significant impact on population health, economic productivity, and tourism in Australia. This study examined epidemiologic patterns of RRV disease in Queensland, Australia, during January 2001–December 2011 at a statistical local area level. Spatio-temporal analyses were used to identify the patterns of the disease distribution over time stratified by age, sex, and space. The results show that the mean annual incidence was 54 per 100,000 persons, with a male:female ratio of 1:1.1. Two space-time clusters were identified: the areas adjacent to Townsville, on the eastern coast of Queensland, and the southeast areas. Thus, although public health intervention should be considered across all areas in which RRV occurs, it should specifically focus on high-risk regions, particularly during summer and autumn to reduce the social and economic impacts of RRV infection. PMID:24799374

Cell-cell transmission of VSV-G pseudotyped lentivector particles.

Directory of Open Access Journals (Sweden)

Amy M Skinner

Full Text Available Many replicating viruses, including HIV-1 and HTLV-1, are efficiently transmitted from the cell surface of actively infected cells upon contact with bystander cells. In a previous study, we reported the prolonged cell surface retention of VSV-G replication-deficient pseudotyped lentivector prior to endocytic entry. However, the competing kinetics of cell surface versus dissociation, neutralization or direct transfer to other cells have received comparatively little attention. Here we demonstrate that the relative efficiency of cell-cell surface transmission can outpace "cell-free" transduction at limiting vector input. This coincides with the prolonged half-life of cell bound vector but occurs, unlike HTLV-1, without evidence for particle aggregation. These studies suggest that cell-surface attachment stabilizes particles and alters neutralization kinetics. Our experiments provide novel insight into the underexplored cell-cell transmission of pseudotyped particles.

Ross - for altid?

DEFF Research Database (Denmark)

Blume, Peter Erik

2011-01-01

Med baggrund i Jens Ewalds bog om Alf Ross omtaler artiklen en række de problemstillinger, som bogen behandler, samt vurderer den betydning, Ross har haft og måske fortsat har. Afslutningsvis betones den juridiske biografis betydning.......Med baggrund i Jens Ewalds bog om Alf Ross omtaler artiklen en række de problemstillinger, som bogen behandler, samt vurderer den betydning, Ross har haft og måske fortsat har. Afslutningsvis betones den juridiske biografis betydning....

Sindbis Virus-Pseudotyped Lentiviral Vectors Carrying VEGFR2-Specific Nanobody for Potential Transductional Targeting of Tumor Vasculature.

Science.gov (United States)

Ahani, Roshank; Roohvand, Farzin; Cohan, Reza Ahangari; Etemadzadeh, Mohammad Hossein; Mohajel, Nasir; Behdani, Mahdi; Shahosseini, Zahra; Madani, Navid; Azadmanesh, Kayhan

2016-11-01

Introduction of selectivity/specificity into viral-based gene delivery systems, such as lentiviral vectors (LVs), is crucial in their systemic administration for cancer gene therapy. The pivotal role of tumor-associated endothelial cells (TAECs) in tumor angiogenesis and overexpression of vascular endothelial growth factor receptor-2 (VEGFR2 or KDR) in TAECs makes them a potent target in cancer treatment. Herein, we report the development of VEGFR2-targeted LVs pseudotyped with chimeric sindbis virus E2 glycoprotein (cSVE2s). For this purpose, either sequence of a VEGFR2-specific nanobody or its natural ligand (VEGF 121 ) was inserted into the binding site of sindbis virus E2 glycoprotein. In silico modeling data suggested that the inserted targeting motifs were exposed in the context of cSVE2s. Western blot analysis of LVs indicated the incorporation of cSVE2s into viral particles. Capture ELISA demonstrated the specificity/functionality of the incorporated cSVE2s. Transduction of 293/KDR (expressing VEGFR2) or 293T cells (negative control) by constructed LVs followed by fluorescent microscopy and flow cytometric analyses indicated selective transduction of 293/KDR cells (30 %) by both targeting motifs compared to 293T control cells (1-2 %). These results implied similar targeting properties of VEGFR2-specific nanobody compared to the VEGF 121 and indicated the potential for transductional targeting of tumor vasculature by the nanobody displaying LVs.

Analysis of proteins of mouse sarcoma pseudotype viruses: type-specific radioimmunoassays for ecotropic virus p30's

International Nuclear Information System (INIS)

Kennel, S.J.; Tennant, R.W.

1979-01-01

Murine sarcoma virus pseudotypes were prepared by infection of nonproducer cells (A1-2), which were transformed by the Gazdar strain of mouse sarcoma virus, with Gross (N-tropic), WN1802B (B-tropic), or Moloney (NB-tropic) viruses. The respective host range pseudotype sarcoma viruses were defined by the tritration characteristics on cells with the appropriate Fv-1 genotype. Proteins from virus progeny were analyzed by sodium dodecyl sulfate--polyacrylamide gel electrophoresis. Bands present in both the 65,000- and the 10,000- to 20,000-molecular-weight regions of the gel distinguished the pseudotype viruses from their respective helpers. Furthermore, two protein bands were noted in the p30 region of murine sarcoma virus (Gross), one corresponding to Gross virus p30, and another of slightly slower mobility. However, since the mobility of the putative sarcoma p30 is nearly indentical to that of WN1802B, its presence could not be established by sodium dodecyl sulfate--polyacrylamide gel electrophoresis. Type-specific radioimmunossays for Gross virus p30 and for WN1802B p30 were applied for analysis of pseudotype preparations, and among several ecotropic viruses tested, only the homologous virus scored in the respective assay. By use of these assays, pseudotype viruses were found to contain only 8 to 48% helper-specific p30's; the remainder is presumably derived from the sarcoma virus

Novel cyclo-peptides inhibit Ebola pseudotyped virus entry by targeting primed GP protein.

Science.gov (United States)

Li, Quanjie; Ma, Ling; Yi, Dongrong; Wang, Han; Wang, Jing; Zhang, Yongxin; Guo, Ying; Li, Xiaoyu; Zhou, Jinming; Shi, Yi; Gao, George F; Cen, Shan

2018-07-01

Ebola virus (EBOV) causes fatal hemorrhagic fever with high death rates in human. Currently, there are no available clinically-approved prophylactic or therapeutic treatments. The recently solved crystal structure of cleavage-primed EBOV glycoprotein (GPcl) in complex with the C domain of endosomal protein Niemann-Pick C1 (NPC1) provides a new target for the development of EBOV entry inhibitors. In this work, a computational approach using docking and molecular dynamic simulations is carried out for the rational design of peptide inhibitors. A novel cyclo-peptide (Pep-3.3) was identified to target at the late stage of EBOV entry and exhibit specific inhibitory activity against EBOV-GP pseudotyped viruses, with 50% inhibitory concentration (IC50) of 5.1 μM. In vitro binding assay and molecular simulations revealed that Pep-3.3 binds to GPcl with a KD value of 69.7 μM, through interacting with predicted residues in the hydrophobic binding pocket of GPcl. Mutation of predicted residues T83 caused resistance to Pep-3.3 inhibition in viral infectivity, providing preliminary support for the model of the peptide binding to GPcl. This study demonstrates the feasibility of inhibiting EBOV entry by targeting GPcl with peptides. Copyright © 2018 Elsevier B.V. All rights reserved.

Immunogenicity and efficacy of immunodeficiency virus-like particles pseudotyped with the G protein of vesicular stomatitis virus

International Nuclear Information System (INIS)

Kuate, Seraphin; Stahl-Hennig, Christiane; Stoiber, Heribert; Nchinda, Godwin; Floto, Anja; Franz, Monika; Sauermann, Ulrike; Bredl, Simon; Deml, Ludwig; Ignatius, Ralf; Norley, Steve; Racz, Paul; Tenner-Racz, Klara; Steinman, Ralph M.; Wagner, Ralf; Uberla, Klaus

2006-01-01

Vaccination with exogenous antigens such as recombinant viral proteins, immunodeficiency virus-derived whole inactivated virus particles, or virus-like particles (VLP) has generally failed to provide sufficient protection in animal models for AIDS. Pseudotyping VLPs with the vesicular stomatitis virus G protein (VSV-G), which is known to mediate entry into dendritic cells, might allow more efficient stimulation of immune responses. Therefore, we pseudotyped noninfectious immunodeficiency virus-like particles with VSV-G and carried out a preliminary screen of their immunogenicity and vaccination efficacy. Incorporation of VSV-G into HIV-1 VLPs led to hundred-fold higher antibody titers to HIV-1 Gag and enhancement of T cell responses in mice. Repeated vaccination of rhesus monkeys for 65 weeks with VSV-G pseudotyped simian immunodeficiency virus (SIV)-like particles (VLP[G]) provided initial evidence for efficient suppression of viral load after mucosal challenge with the SIVmac239 virus. Challenge of monkeys after a 28 week vaccination regimen with VLP[G] led to a reduction in peak viremia, but persistent suppression of viral load was not achieved. Due to limitations in the number of animals available for this study, improved efficacy of VSV-G pseudotyped VLPs in nonhuman primates could not be demonstrated. However, mouse experiments revealed that pseudotyping of VLPs with fusion-competent VSV-G clearly improves their immunogenicity. Additional strategies, particularly adjuvants, should be considered to provide greater protection against a challenge with pathogenic immunodeficiency virus

Ross Versus Non-Ross Aortic Valve Replacement in Children: A 22-Year Single Institution Comparison of Outcomes.

Science.gov (United States)

Brown, John W; Patel, Parth M; Ivy Lin, Jiuann-Huey; Habib, Asma S; Rodefeld, Mark D; Turrentine, Mark W

2016-05-01

The Ross aortic valve replacement (AVR) has been the AVR of choice for children at our center since 1993. Absence or inadequate quality of the pulmonary valve or, less commonly, family or surgeon preference caused us to select an alternative AVR prosthesis for some children. This review compares the outcomes of 42 children who received a non-Ross AVR with 115 children undergoing Ross root replacement at our institution during the most recent 22 years. A retrospective chart review of the 42 pediatric non-Ross AVRs was compared with 115 Ross AVRs. The mean age at AVR was 11.0 ± 6.5 years (range, 1 month to 18 years) for the non-Ross and 11.4 ± 5.5 years (range, 6 weeks to 18 years) for the Ross groups. Follow-up was 7.8 ± 6.2 years for the Ross group and 8.7 ± 6.5 years for the non-Ross group. The Ross AVR technique was modified in 2000; these modifications were applied to 72 patients (63%) of the total Ross AVR group. All-cause early and late mortality of the non-Ross AVR group was 17% compared with 4% for the Ross cohort (p = 0.017). The actuarial survival rate at 20 years for the non-Ross AVR group was 81% compared with 94% for the Ross group (p = 0.018). Reintervention rate was 29% (12/38) in the non-Ross group and 28% (32/115) in the entire Ross group (60% [26/43] before 2000, and 8% [6/72] after 2000). Thromboemboli, bleeding, and endocarditis occurred in 2.4%, 1.4%, and 10% in the non-Ross group compared with 0.9%, 2.6%, and 1.7% in the Ross AVR group (p = 0.46, 0.01, and 0.02), respectively. Patients in the non-Ross group had a significantly higher recurrent or persistent aortic valve gradient (>20 mm Hg) than did patients in the Ross group at most recent follow-up (p Ross AVR than with other types of AVR prostheses. The Ross AVR remains the treatment of choice for children who have an adequate pulmonary valve. Reoperation for Ross root dilatation, regurgitation, or both (only 6% since our modifications in 2000) has markedly reduced the main drawbacks

Ross og demokratiet

DEFF Research Database (Denmark)

Svensson, Palle

2006-01-01

En kritisk analyse af Alf Ross' teori om demokrati på grundlag af Roberty A. Dahl's begrebsapparat.......En kritisk analyse af Alf Ross' teori om demokrati på grundlag af Roberty A. Dahl's begrebsapparat....

Bayesian Spatiotemporal Analysis of Socio-Ecologic Drivers of Ross River Virus Transmission in Queensland, Australia

Science.gov (United States)

Hu, Wenbiao; Clements, Archie; Williams, Gail; Tong, Shilu; Mengersen, Kerrie

2010-01-01

This study aims to examine the impact of socio-ecologic factors on the transmission of Ross River virus (RRV) infection and to identify areas prone to social and ecologic-driven epidemics in Queensland, Australia. We used a Bayesian spatiotemporal conditional autoregressive model to quantify the relationship between monthly variation of RRV incidence and socio-ecologic factors and to determine spatiotemporal patterns. Our results show that the average increase in monthly RRV incidence was 2.4% (95% credible interval (CrI): 0.1–4.5%) and 2.0% (95% CrI: 1.6–2.3%) for a 1°C increase in monthly average maximum temperature and a 10 mm increase in monthly average rainfall, respectively. A significant spatiotemporal variation and interactive effect between temperature and rainfall on RRV incidence were found. No association between Socio-economic Index for Areas (SEIFA) and RRV was observed. The transmission of RRV in Queensland, Australia appeared to be primarily driven by ecologic variables rather than social factors. PMID:20810846

Rossing update

International Nuclear Information System (INIS)

Griebel, E.G.

2000-01-01

Rossing Uranium, along with the rest of the industry, has been undergoing major changes to adjust to the increasingly competitive uranium market in the 1990's. This paper will outline the developments during the 1990's at the mine, as well as current projects. Rossing has successfully managed the transition through a phase of very low production in the first half of the 1990's, and is now operating at 70% of capacity. During this phase the mine was also able to implement tailings and water management strategies which have reduced fresh water requirements to historical lows, whilst preserving process performance despite cessation of iron oxide supplies. Capital investment in mining equipment, a pre-screening facility in the Fine Crushing plant, and currently an ore sorting pilot plant have been made to enhance Rossing's ability to lower costs further. Rossing is in the middle of a business improvement programme aimed at cutting unit costs in excess of 20%, whilst continuing to improve safety, health and environmental performance. Key focus areas of this programme are maintenance strategy, equipment and plant utilisation and people development. (author)

Comparison of the Ross/Ross-Konno aortic root in children before and after the age of 18 months.

Science.gov (United States)

Lo Rito, Mauro; Davies, Ben; Brawn, William J; Jones, Timothy J; Khan, Natasha; Stickley, John; Barron, David J

2014-09-01

Evaluation of long-term results after the Ross/Ross-Konno operation in patients Ross/Ross-Konno operation (1991-2011). Data were obtained from hospital records and follow-up was 100% complete. Between January 1991 and December 2011, 140 patients underwent the Ross/Ross-Konno operation and 22 patients were Ross operation and 8 patients a Ross-Konno operation. Presentation at surgery was aortic valve stenosis in 13, regurgitation in 7 and mixed disease in 2. Only 4 patients (18%) had no surgery prior to Ross/Ross-Konno, and among the others the previous most frequent operation was aortic valvotomy (55%). There were 3 early deaths, all in high-risk cases with poor preoperative left ventricular function. At discharge there was no neoaortic regurgitation in 10, trivial in 4 and mild in 5. The median follow-up is 10.8 years (range 0.96-21). There was 1 late death due to progressive ventricular dysfunction 2.4 years after Ross-Konno and mitral valve replacement. Survival for patients 18-months patients (P Ross/Ross-Konno can be invaluable in the younger age group but not without risk in the setting of ventricular dysfunction. Long-term performance of the neoaortic valve is significantly better than in older children, which is related to maintenance of normal root dimensions compared with progressive dilatation in patients undergoing Ross/Ross-Konno at older ages. © The Author 2014. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

Alf Ross

DEFF Research Database (Denmark)

Ryberg, Jesper

En (overvejende) ny generation af akademikere møder og prøver Ross' tanker om emner som retfærdighed, demokrati, statsforfatningsret, ytringsfrihed, straf, erkendelsesteori, etik, logik, fri vilje og sygdom.......En (overvejende) ny generation af akademikere møder og prøver Ross' tanker om emner som retfærdighed, demokrati, statsforfatningsret, ytringsfrihed, straf, erkendelsesteori, etik, logik, fri vilje og sygdom....

Bandura, Ross, and Ross: Observational Learning and the Bobo Doll

Science.gov (United States)

Artino, Anthony R., Jr.

2007-01-01

Since the publication of their seminal article entitled, "Transmission of Aggression Through Imitation of Aggressive Models" (Bandura, Ross, & Ross, 1961), the work of Albert Bandura and his co-authors has had an immeasurable impact on the field of psychology, in general, and educational psychology, more specifically. The purpose of this report is…

Ross og erkendelsesteorien

DEFF Research Database (Denmark)

Holtermann, Jakob von Holderstein

2006-01-01

Et argument for at den logiske positivismes notoriske problemer ikke - som almindeligt antaget - har gjort Alf Ross' retsfilosofi forældet. Mere konkret påvises det at Ross i virkeligheden passer bedre sammen med den empiriske erkendelsesteori der har rejst sig på den logiske positivismes ruiner....

Does the Homograft for RVOT Reconstruction in Ross: Patients Fare Better than for Non-Ross Patients? A Single-Center Experience.

Science.gov (United States)

Ruzmetov, Mark; Geiss, Dale M; Shah, Jitendra J; Fortuna, Randall S; Welke, Karl F

2015-07-01

In patients undergoing the Ross procedure, the right ventricular outflow tract (RVOT) conduit is inserted in an orthotopic position rather than in the more heterotopic position used in the repair of complex congenital RVOT obstruction. The study aim was to compare the authors' institutional mid-term experience of large-sized homografts (>19 mm) in patients with Ross and non-Ross RVOT reconstructions. The outcome was reviewed of all homografts implanted for Ross (n = 72) or non-Ross (n = 64) RVOT reconstruction at a single center between 1993 and 2012. Echocardiographic data were reviewed to evaluate valve performance. Homograft dysfunction was defined as RVOT obstruction with peak echo-Doppler gradient >40 mmHg and/or grade >III/IV conduit valve regurgitation. Homograft failure was defined as the need for conduit replacement or catheter or surgical reintervention. The age, body weight, conduit diameter and previous surgery were significantly higher in patients with Ross compared to the non-Ross group (p = 0.002, 0.003 and Ross, n = 17; non-Ross, n = 18). The data acquired showed actuarial survival, freedom from conduit dysfunction and conduit failure to be similar in both cohorts. Freedom from any type of reoperation was worse for the Ross group (58%) than for the non-Ross group (72%) (p = 0.05). During the first 15 years after Ross or non-Ross pulmonary homograft implantation, the survival rate, freedom from failure and dysfunction, and RVOT gradient were statistically similar. Freedom from any type of reoperation was significantly higher in the non-Ross group, however.

The Ross Procedure in Pediatric Patients: A 20-Year Experience of Ross Procedure in a Single Institution.

Science.gov (United States)

Yoon, Dong Woog; Yang, Ji-Hyuk; Jun, Tae-Gook; Park, Pyo Won

2017-08-01

The Ross/Ross-Konno procedure is considered a good option for irreparable aortic valve disease in pediatric patients because of its hemodynamic performance and potential for growth of the pulmonary autograft. This study is a review of the long-term results of our 20-year experience with the Ross and Ross-Konno operations in a single institution. Between June 1995 and January 2016, 16 consecutive patients (mean age, 6.0±5.9 years; range, 16 days to 17.4 years) underwent either a Ross operation (n=9) or a Ross-Konno operation (n=7). The study included 12 males and 4 females, with a median follow-up period of 47 months (range, 6 to 256 months). There were no cases of in-hospital or late mortality. Six reoperations were performed in 5 patients. Four patients underwent right ventricular-pulmonary artery (RV-PA) conduit replacement. Two patients underwent concomitant replacement of the pulmonary autograft and RV-PA conduit 10 years and 8 years after the Ross operation, respectively. The rate of freedom from adverse outcomes of the pulmonary autograft was 88% and 70% at 5 and 10 years, respectively. The rate of freedom from valve-related reoperations was 79% and 63% at 5 and 10 years, respectively. Pulmonary autografts demonstrated good durability with low mortality. The Ross/Ross-Konno procedure is a good option that can be performed safely in pediatric patients with aortic valve disease, even in a small-volume center.

Resting lymphocyte transduction with measles virus glycoprotein pseudotyped lentiviral vectors relies on CD46 and SLAM

International Nuclear Information System (INIS)

Zhou Qi; Schneider, Irene C.; Gallet, Manuela; Kneissl, Sabrina; Buchholz, Christian J.

2011-01-01

The measles virus (MV) glycoproteins hemagglutinin (H) and fusion (F) were recently shown to mediate transduction of resting lymphocytes by lentiviral vectors. MV vaccine strains use CD46 or signaling lymphocyte activation molecule (SLAM) as receptor for cell entry. A panel of H protein mutants derived from vaccine strain or wild-type MVs that lost or gained CD46 or SLAM receptor usage were investigated for their ability to mediate gene transfer into unstimulated T lymphocytes. The results demonstrate that CD46 is sufficient for efficient vector particle association with unstimulated lymphocytes. For stable gene transfer into these cells, however, both MV receptors were found to be essential.

Climate variation and incidence of Ross river virus in Cairns, Australia: a time-series analysis.

Science.gov (United States)

Tong, S; Hu, W

2001-01-01

In this study we assessed the impact of climate variability on the Ross River virus (RRv) transmission and validated an epidemic-forecasting model in Cairns, Australia. Data on the RRv cases recorded between 1985 and 1996 were obtained from the Queensland Department of Health. Climate and population data were supplied by the Australian Bureau of Meteorology and the Australian Bureau of Statistics, respectively. The cross-correlation function (CCF) showed that maximum temperature in the current month and rainfall and relative humidity at a lag of 2 months were positively and significantly associated with the monthly incidence of RRv, whereas relative humidity at a lag of 5 months was inversely associated with the RRv transmission. We developed autoregressive integrated moving average (ARIMA) models on the data collected between 1985 to 1994, and then validated the models using the data collected between 1995 and 1996. The results show that the relative humidity at a lag of 5 months (p < 0.001) and the rainfall at a lag of 2 months (p < 0.05) appeared to play significant roles in the transmission of RRv disease in Cairns. Furthermore, the regressive forecast curves were consistent with the pattern of actual values. PMID:11748035

Rossing set for great future

International Nuclear Information System (INIS)

Anon.

1978-01-01

Rio Tinto's Rossing open pit operation - the world's largest uranium mine - has had difficult birth pangs. The teething troubles which beset the mine are examined, together with the mining and extraction methods employed. Rossing's enlightened ideas on the labour question are also covered

Rossing

International Nuclear Information System (INIS)

Anon.

1979-01-01

Rossing is a uranium mine in the heart of the Namib desert in South West Africa (Namibia). Interms of the material recovered from the huge open pit, it is the largest uranium mine in the world. This article gives a short history of the development of this mine

Enhancement of Ebola Virus Infection via Ficolin-1 Interaction with the Mucin Domain of GP Glycoprotein.

Science.gov (United States)

Favier, Anne-Laure; Gout, Evelyne; Reynard, Olivier; Ferraris, Olivier; Kleman, Jean-Philippe; Volchkov, Viktor; Peyrefitte, Christophe; Thielens, Nicole M

2016-06-01

Ebola virus infection requires the surface viral glycoprotein to initiate entry into the target cells. The trimeric glycoprotein is a highly glycosylated viral protein which has been shown to interact with host C-type lectin receptors and the soluble complement recognition protein mannose-binding lectin, thereby enhancing viral infection. Similarly to mannose-binding lectin, ficolins are soluble effectors of the innate immune system that recognize particular glycans at the pathogen surface. In this study, we demonstrate that ficolin-1 interacts with the Zaire Ebola virus (EBOV) glycoprotein, and we characterized this interaction by surface plasmon resonance spectroscopy. Ficolin-1 was shown to bind to the viral glycoprotein with a high affinity. This interaction was mediated by the fibrinogen-like recognition domain of ficolin-1 and the mucin-like domain of the viral glycoprotein. Using a ficolin-1 control mutant devoid of sialic acid-binding capacity, we identified sialylated moieties of the mucin domain to be potential ligands on the glycoprotein. In cell culture, using both pseudotyped viruses and EBOV, ficolin-1 was shown to enhance EBOV infection independently of the serum complement. We also observed that ficolin-1 enhanced EBOV infection on human monocyte-derived macrophages, described to be major viral target cells,. Competition experiments suggested that although ficolin-1 and mannose-binding lectin recognized different carbohydrate moieties on the EBOV glycoprotein, the observed enhancement of the infection likely depended on a common cellular receptor/partner. In conclusion, ficolin-1 could provide an alternative receptor-mediated mechanism for enhancing EBOV infection, thereby contributing to viral subversion of the host innate immune system. A specific interaction involving ficolin-1 (M-ficolin), a soluble effector of the innate immune response, and the glycoprotein (GP) of EBOV was identified. Ficolin-1 enhanced virus infection instead of tipping the

The Ross Sea Dipole - temperature, snow accumulation and sea ice variability in the Ross Sea region, Antarctica, over the past 2700 years

Science.gov (United States)

Bertler, Nancy A. N.; Conway, Howard; Dahl-Jensen, Dorthe; Emanuelsson, Daniel B.; Winstrup, Mai; Vallelonga, Paul T.; Lee, James E.; Brook, Ed J.; Severinghaus, Jeffrey P.; Fudge, Taylor J.; Keller, Elizabeth D.; Baisden, W. Troy; Hindmarsh, Richard C. A.; Neff, Peter D.; Blunier, Thomas; Edwards, Ross; Mayewski, Paul A.; Kipfstuhl, Sepp; Buizert, Christo; Canessa, Silvia; Dadic, Ruzica; Kjær, Helle A.; Kurbatov, Andrei; Zhang, Dongqi; Waddington, Edwin D.; Baccolo, Giovanni; Beers, Thomas; Brightley, Hannah J.; Carter, Lionel; Clemens-Sewall, David; Ciobanu, Viorela G.; Delmonte, Barbara; Eling, Lukas; Ellis, Aja; Ganesh, Shruthi; Golledge, Nicholas R.; Haines, Skylar; Handley, Michael; Hawley, Robert L.; Hogan, Chad M.; Johnson, Katelyn M.; Korotkikh, Elena; Lowry, Daniel P.; Mandeno, Darcy; McKay, Robert M.; Menking, James A.; Naish, Timothy R.; Noerling, Caroline; Ollive, Agathe; Orsi, Anaïs; Proemse, Bernadette C.; Pyne, Alexander R.; Pyne, Rebecca L.; Renwick, James; Scherer, Reed P.; Semper, Stefanie; Simonsen, Marius; Sneed, Sharon B.; Steig, Eric J.; Tuohy, Andrea; Ulayottil Venugopal, Abhijith; Valero-Delgado, Fernando; Venkatesh, Janani; Wang, Feitang; Wang, Shimeng; Winski, Dominic A.; Winton, V. Holly L.; Whiteford, Arran; Xiao, Cunde; Yang, Jiao; Zhang, Xin

2018-02-01

High-resolution, well-dated climate archives provide an opportunity to investigate the dynamic interactions of climate patterns relevant for future projections. Here, we present data from a new, annually dated ice core record from the eastern Ross Sea, named the Roosevelt Island Climate Evolution (RICE) ice core. Comparison of this record with climate reanalysis data for the 1979-2012 interval shows that RICE reliably captures temperature and snow precipitation variability in the region. Trends over the past 2700 years in RICE are shown to be distinct from those in West Antarctica and the western Ross Sea captured by other ice cores. For most of this interval, the eastern Ross Sea was warming (or showing isotopic enrichment for other reasons), with increased snow accumulation and perhaps decreased sea ice concentration. However, West Antarctica cooled and the western Ross Sea showed no significant isotope temperature trend. This pattern here is referred to as the Ross Sea Dipole. Notably, during the Little Ice Age, West Antarctica and the western Ross Sea experienced colder than average temperatures, while the eastern Ross Sea underwent a period of warming or increased isotopic enrichment. From the 17th century onwards, this dipole relationship changed. All three regions show current warming, with snow accumulation declining in West Antarctica and the eastern Ross Sea but increasing in the western Ross Sea. We interpret this pattern as reflecting an increase in sea ice in the eastern Ross Sea with perhaps the establishment of a modern Roosevelt Island polynya as a local moisture source for RICE.

Rossing uranium

International Nuclear Information System (INIS)

Anon.

1979-01-01

In this article the geology of the deposits of the Rossing uranium mine in Namibia is discussed. The planning of the open-pit mining, the blasting, drilling, handling and the equipment used for these processes are described

MR diagnostics after Ross procedure

International Nuclear Information System (INIS)

Steffens, J.C.; Link, J.; Harringer, W.; Haverich, A.; Heller, M.

1996-01-01

Purpose: To determine the ability of MRI to diagnose morphological and functional changes in patients after Ross procedure. Material and methods: During one year, 5 patients after Ross procedure were studied by MRI. T 1 -weighted spin-echo sequences in different orientations as well as cine sequences and a VENC-sequence were used. Results: In all patients the morphology including the postoperative changes could be visualised by MRI. The cine sequences provided functional information on valvular and ventricular function. VENC-MR yielded an exact quantification of the regurgitant fraction. Conclusion: MRI is capable of providing all important diagnostic information in patients after Ross procedure such as valvular and ventricular function and valvular and outflow tract morphology in a single examination. (orig.) [de

The South Pole and the Ross Sea

Science.gov (United States)

2002-01-01

This image shows a rare clear view of the South Pole (lower right) and the Ross Sea, Antarctica. The Sea-viewing Wide Field-of-view Sensor (SeaWiFS) acquired the scene on December 26, 2001. The geographic South Pole is located in the center of Antarctica, at an altitude of 2,900 meters (9,300 feet). It rests on a continent-wide ice sheet that is 2,870 m thick, with the underlying bedrock only 30 m (98 feet) above sea level. The ice underlying the South Pole is as much as 140,000 years old, and is currently accumulating at about 82 cm (32 inches) per year. Roughly 2,500 km (1,550 miles) away is the green water of the Ross Sea, which indicates the presence of large numbers of phytoplankton. This is a highly productive part of the world's oceans. Also note the ice gathered around McMurdo Sound, seen toward the lefthand shoreline of the Ross Sea, at the edge of the Ross Ice Shelf. According to National Science Foundation researchers, this ice is making it difficult for penguins to reach their food supply. Separating the continental Antarctic ice sheet from the Ross Sea are the Queen Maud Mountains and the Ross Ice Shelf. Image courtesy the SeaWiFS Project, NASA/Goddard Space Flight Center, and ORBIMAGE

The Ross Sea Dipole – temperature, snow accumulation and sea ice variability in the Ross Sea region, Antarctica, over the past 2700 years

Directory of Open Access Journals (Sweden)

N. A. N. Bertler

2018-02-01

Full Text Available High-resolution, well-dated climate archives provide an opportunity to investigate the dynamic interactions of climate patterns relevant for future projections. Here, we present data from a new, annually dated ice core record from the eastern Ross Sea, named the Roosevelt Island Climate Evolution (RICE ice core. Comparison of this record with climate reanalysis data for the 1979–2012 interval shows that RICE reliably captures temperature and snow precipitation variability in the region. Trends over the past 2700 years in RICE are shown to be distinct from those in West Antarctica and the western Ross Sea captured by other ice cores. For most of this interval, the eastern Ross Sea was warming (or showing isotopic enrichment for other reasons, with increased snow accumulation and perhaps decreased sea ice concentration. However, West Antarctica cooled and the western Ross Sea showed no significant isotope temperature trend. This pattern here is referred to as the Ross Sea Dipole. Notably, during the Little Ice Age, West Antarctica and the western Ross Sea experienced colder than average temperatures, while the eastern Ross Sea underwent a period of warming or increased isotopic enrichment. From the 17th century onwards, this dipole relationship changed. All three regions show current warming, with snow accumulation declining in West Antarctica and the eastern Ross Sea but increasing in the western Ross Sea. We interpret this pattern as reflecting an increase in sea ice in the eastern Ross Sea with perhaps the establishment of a modern Roosevelt Island polynya as a local moisture source for RICE.

Marc Ross

Indian Academy of Sciences (India)

Marc Ross. Articles written in Pramana – Journal of Physics. Volume 69 Issue 6 December 2007 pp 1147-1150 Machine Detector Interface. A laser-wire system for the International Linear Collider · Nicolas Delerue Sudhir Dixit Fred Gannaway David Howell Myriam Qurshi Grahame Blair Stewart Boogert Gary Boorman ...

Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light.

Science.gov (United States)

Faddy, Helen M; Fryk, Jesse J; Prow, Natalie A; Watterson, Daniel; Young, Paul R; Hall, Roy A; Tolksdorf, Frank; Sumian, Chryslain; Gravemann, Ute; Seltsam, Axel; Marks, Denese C

2016-06-01

Arboviruses, including dengue (DENV 1-4), chikungunya (CHIKV), and Ross River (RRV), are emerging viruses that are a risk for transfusion safety globally. An approach for managing this risk is pathogen inactivation, such as the THERAFLEX UV-Platelets system. We investigated the ability of this system to inactivate the above mentioned arboviruses. DENV 1-4, CHIKV, or RRV were spiked into buffy coat (BC)-derived platelet (PLT) concentrates in additive solution and treated with the THERAFLEX UV-Platelets system at the following doses: 0.05, 0.1, 0.15, and 0.2 J/cm(2) (standard dose). Pre- and posttreatment samples were taken for each dose, and the level of viral infectivity was determined. At the standard ultraviolet C (UVC) dose (0.2 J/cm(2) ), viral inactivation of at least 4.43, 6.34, and 5.13 log or more, was observed for DENV 1-4, CHIKV, and RRV, respectively. A dose dependency in viral inactivation was observed with increasing UVC doses. Our study has shown that DENV, CHIKV, and RRV, spiked into BC-derived PLT concentrates, were inactivated by the THERAFLEX UV-Platelets system to the limit of detection of our assay, suggesting that this system could contribute to the safety of PLT concentrates with respect to these emerging arboviruses. © 2016 AABB.

Major adverse cardiac and cerebrovascular events after the ross procedure: A report from the german-dutch ross registry

NARCIS (Netherlands)

H. Sievers (Hans Hinrich); U. Stierle (Ulrich); E.I. Charitos (Efstratios); T. Hanke; M. Misfeld (Martin); J.F.M. Bechtel (Matthias); A. Gorski (Armin); U.F. Franke (Ulrich); B. Graf (Bernhard); D.R. Robinson (Derek); A.J.J.C. Bogers (Ad); A. Dodge-Khatami (Ali); J.O. Boehm (Juergen); J.G. Rein (Joachim); C.A. Botha (Cornelius); R. Lange (Rüdiger); J. Hoerer (Juergen); A. Moritz (Anton); T. Wahlers (Thorsten); M. Breuer (Martin); K. Ferrari-Kuehne (Katharina); R. Hetzer (Roland); M. Huebler (Michael); G. Ziemer (Gerhard); J.J.M. Takkenberg (Hanneke); W. Hemmer

2010-01-01

textabstractBackground-: The purpose of the study is to report major cardiac and cerebrovascular events after the Ross procedure in the large adult and pediatric population of the German-Dutch Ross registry. These data could provide an additional basis for discussions among physicians and a source

36 CFR 7.69 - Ross Lake National Recreation Area.

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Ross Lake National Recreation... INTERIOR SPECIAL REGULATIONS, AREAS OF THE NATIONAL PARK SYSTEM § 7.69 Ross Lake National Recreation Area... snowmobiles the following locations within the Ross Lake National Recreation Area: (1) State Highway 20, that...

STS-110 Crew Interview: Jerry Ross

Science.gov (United States)

2002-01-01

STS-110 Mission Specialist Jerry Ross is seen during this preflight interview, where he gives a quick overview of the mission before answering questions about his inspiration to become an astronaut and his career path. Ross outlines his role in the mission in general, and specifically during the docking and extravehicular activities (EVAs). He describes the payload (S0 Truss and Mobile Transporter) and the dry run installation of the S0 truss that will take place the day before the EVA for the actual installation. Ross discusses the planned EVAs in detail and outlines what supplies will be left for the resident crew of the International Space Station (ISS). He ends with his thoughts on the most valuable aspect of the ISS.

Changing Roots : Remodelling after the Ross Operation

NARCIS (Netherlands)

A. Mookhoek (Aart)

2016-01-01

textabstractThe Ross operation is a surgical intervention in which a diseased aortic valve is replaced by the pulmonary valve of the same patient. A donor valve replaces the pulmonary valve. In this thesis, several key unanswered clinical questions are explored. Early mortality after the Ross

The Externally Supported Ross Operation: Early Outcomes and Intermediate Follow-Up.

Science.gov (United States)

Jacobsen, Roni M; Earing, Michael G; Hill, Garick D; Barnes, Michael; Mitchell, Michael E; Woods, Ronald K; Tweddell, James S

2015-08-01

The externally supported Ross (supported Ross), consisting of a Dacron (DuPont, Wilmington, DE) graft to support the neoaortic root, has been used in adolescent and adult patients to prevent neoaortic dilatation. Outcomes after the supported Ross technique were compared with the Ross procedure using the standard aortic root replacement technique (standard Ross). This was a retrospective analysis of 36 adolescent and young adult patients who underwent the Ross procedure between 1992 and 2013. The outcomes of supported Ross procedures in 26 patients were compared with the Ross procedure in 10 patients. End points included survival, neoaortic root dilatation, development of neoaortic regurgitation, and the need for reintervention. The median age at operation was 14 years (range, 11 to 31 years), and indications for the operation were mixed lesions (47%), followed by aortic regurgitation (42%) and stenosis (11%). There were no early deaths. The mean follow-up was 2.2 years (range, 1 to 11 years). At the 1-year (p = 0.01) and 3-year (p Ross cohort had a smaller neoaortic root z-score. Neither cohort had a large number of patients with significant neoaortic regurgitation, with 1 patient in the supported cohort compared with 3 patients in the standard cohort. Overall, 4 patients (40%) in the standard Ross cohort had required reintervention, including 3 directed at the neoaortic root. One patient in the supported Ross cohort required early reintervention for revision of the right coronary artery. At intermediate follow-up, patients who underwent the supported Ross technique were less likely to have neoaortic root dilatation compared with patients who underwent a standard Ross procedure. Further studies are needed to evaluate the long-term durability of this technique, particularly in regards to the development of significant aortic regurgitation, the rate of reintervention, and application to younger and smaller patients. Copyright © 2015 The Society of Thoracic Surgeons

Elisabeth Kubler-Ross as a Religious Leader.

Science.gov (United States)

Klass, Dennis; Hutch, Richard A.

1986-01-01

Considers Elisabeth Kubler-Ross as a charismatic religious leader and examines the feminine quality of her message and leadership style. An examination of the prospects for an enduring cultural innovation based on Kubler-Ross's work concludes that her leadership does not conform to conditions necessary for institutionalization of her charismatic…

RD-MolPack technology for the constitutive production of self-inactivating lentiviral vectors pseudotyped with the nontoxic RD114-TR envelope.

Science.gov (United States)

Marin, Virna; Stornaiuolo, Anna; Piovan, Claudia; Corna, Stefano; Bossi, Sergio; Pema, Monika; Giuliani, Erica; Scavullo, Cinzia; Zucchelli, Eleonora; Bordignon, Claudio; Rizzardi, Gian Paolo; Bovolenta, Chiara

2016-01-01

To date, gene therapy with transiently derived lentivectors has been very successful to cure rare infant genetic diseases. However, transient manufacturing is unfeasible to treat adult malignancies because large vector lots are required. By contrast, stable manufacturing is the best option for high-incidence diseases since it reduces the production cost, which is the major current limitation to scale up the transient methods. We have previously developed the proprietary RD2-MolPack technology for the stable production of second-generation lentivectors, based on the RD114-TR envelope. Of note, opposite to vesicular stomatitis virus glycoprotein (VSV-G) envelope, RD114-TR does not need inducible expression thanks to lack of toxicity. Here, we present the construction of RD2- and RD3-MolPack cells for the production of self-inactivating lentivectors expressing green fluorescent protein (GFP) as a proof-of-concept of the feasibility and safety of this technology before its later therapeutic exploitation. We report that human T lymphocytes transduced with self-inactivating lentivectors derived from RD3-MolPack cells or with self-inactivating VSV-G pseudotyped lentivectors derived from transient transfection show identical T-cell memory differentiation phenotype and comparable transduction efficiency in all T-cell subsets. RD-MolPack technology represents, therefore, a straightforward tool to simplify and standardize lentivector manufacturing to engineer T-cells for frontline immunotherapy applications.

Characterization of the receptor-binding domain of Ebola glycoprotein in viral entry.

Science.gov (United States)

Wang, Jizhen; Manicassamy, Balaji; Caffrey, Michael; Rong, Lijun

2011-06-01

Ebola virus infection causes severe hemorrhagic fever in human and non-human primates with high mortality. Viral entry/infection is initiated by binding of glycoprotein GP protein on Ebola virion to host cells, followed by fusion of virus-cell membrane also mediated by GP. Using an human immunodeficiency virus (HIV)-based pseudotyping system, the roles of 41 Ebola GP1 residues in the receptor-binding domain in viral entry were studied by alanine scanning substitutions. We identified that four residues appear to be involved in protein folding/structure and four residues are important for viral entry. An improved entry interference assay was developed and used to study the role of these residues that are important for viral entry. It was found that R64 and K95 are involved in receptor binding. In contrast, some residues such as I170 are important for viral entry, but do not play a major role in receptor binding as indicated by entry interference assay and/or protein binding data, suggesting that these residues are involved in post-binding steps of viral entry. Furthermore, our results also suggested that Ebola and Marburg viruses share a common cellular molecule for entry.

Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes.

Science.gov (United States)

Logan, Nicola; Dundon, William G; Diallo, Adama; Baron, Michael D; James Nyarobi, M; Cleaveland, Sarah; Keyyu, Julius; Fyumagwa, Robert; Hosie, Margaret J; Willett, Brian J

2016-11-11

The measurement of virus-specific neutralising antibodies represents the "gold-standard" for diagnostic serology. For animal morbilliviruses, such as peste des petits ruminants (PPRV) or rinderpest virus (RPV), live virus-based neutralisation tests require high-level biocontainment to prevent the accidental escape of the infectious agents. In this study, we describe the adaptation of a replication-defective vesicular stomatitis virus (VSVΔG) based pseudotyping system for the measurement of neutralising antibodies against animal morbilliviruses. By expressing the haemagglutinin (H) and fusion (F) proteins of PPRV on VSVΔG pseudotypes bearing a luciferase marker gene, neutralising antibody titres could be measured rapidly and with high sensitivity. Serological responses against the four distinct lineages of PPRV could be measured simultaneously and cross-neutralising responses against other morbilliviruses compared. Using this approach, we observed that titres of neutralising antibodies induced by vaccination with live attenuated PPRV were lower than those induced by wild type virus infection and the level of cross-lineage neutralisation varied between vaccinates. By comparing neutralising responses from animals infected with either PPRV or RPV, we found that responses were highest against the homologous virus, indicating that retrospective analyses of serum samples could be used to confirm the nature of the original pathogen to which an animal had been exposed. Accordingly, when screening sera from domestic livestock and wild ruminants in Tanzania, we detected evidence of cross-species infection with PPRV, canine distemper virus (CDV) and a RPV-related bovine morbillivirus, suggesting that exposure to animal morbilliviruses may be more widespread than indicated previously using existing diagnostic techniques. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals.

Science.gov (United States)

Ferns, R Bridget; Tarr, Alexander W; Hue, Stephane; Urbanowicz, Richard A; McClure, C Patrick; Gilson, Richard; Ball, Jonathan K; Nastouli, Eleni; Garson, Jeremy A; Pillay, Deenan

2016-05-01

HIV-1 infected patients who acquire HCV infection have higher rates of chronicity and liver disease progression than patients with HCV mono-infection. Understanding early events in this pathogenic process is important. We applied single genome sequencing of the E1 to NS3 regions and viral pseudotype neutralization assays to explore the consequences of viral quasispecies evolution from pre-seroconversion to chronicity in four co-infected individuals (mean follow up 566 days). We observed that one to three founder viruses were transmitted. Relatively low viral sequence diversity, possibly related to an impaired immune response, due to HIV infection was observed in three patients. However, the fourth patient, after an early purifying selection displayed increasing E2 sequence evolution, possibly related to being on suppressive antiretroviral therapy. Viral pseudotypes generated from HCV variants showed relative resistance to neutralization by autologous plasma but not to plasma collected from later time points, confirming ongoing virus escape from antibody neutralization. Copyright © 2016 Elsevier Inc. All rights reserved.

Isad ja tütred, isad ja pojad / Jüri Ross, Kristine Ross, Fjodor Berman ...[jt.] ; üles kirjutanud Jaana Liigand

Index Scriptorium Estoniae

2011-01-01

Büroomaailma juht Jüri Ross ja turundusdirektor Kristine Ross, BLRT Grupi omanik Fjodor Berman ja BLRT Grupi tütarfirma Refonda tegevdirektor Mark Berman, Saaremaa Laevakompanii ja paljude firmade omanik Vjatšeslav Leedo ja Mosaiigi kohviku omanik Katrin Leedo ärist, juhtimisest ja omavahelistest suhetest

Full production approaching at Rossing Uranium

International Nuclear Information System (INIS)

Anon.

1978-01-01

The Rossing uranium mine, in South West Africa's Namib Desert, is an extraordinary operation by any standards. The open pit mining operation; the highly complex metallurgical plant that, unlike those at mines in South Africa extracts the uranium as far as the uranium oxide or yellowcake product; the back-up services; and the team of people who run Rossing were all seen in action on a recent visit by Coal Gold and Base Minerals. This article examines the geology, the initial exploration and the open pit

Rossing uranium 1979

International Nuclear Information System (INIS)

Anon.

1979-01-01

This report describes the activities and organization of the Rossing uranium mine in South West Africa. The development of the mine during the last six years is described as well as the geology of the uranium deposits and aspects of the mining operations. The manpower structure and training possibilities for personnel are described

High-Resolution Body Wave Tomography of the Ross Sea Embayment, Antarctica

Science.gov (United States)

White-Gaynor, A.; Nyblade, A.; Wiens, D. A.; Aster, R. C.; Gerstoft, P.; Bromirski, P. D.; Stephen, R. A.

2017-12-01

The West Antarctic Rift System (WARS) is one of the least understood continental rift system on the planet. The 1000 km wide WARS includes the Ross Sea Embayment between Marie Byrd Land and the Transantarctic Mountains (TAMS). Active volcanism on Ross Island continues to challenge our understanding of the generally quiescent rift system. Previous regional-scale body wave tomographic investigations have identified areas of low seismic wave speeds to 200 km depth beneath Ross Island. However, the spatial extent of the low velocity structure across the entirety of the WARS remains poorly constrained due to the insufficient resolution of upper mantle structure under the Ross Sea Embayment away from Ross Island. We utilize teleseismic P wave observations recorded on the RIS/DRIS network, which consists of 34 seismometers deployed across the Ross Ice Shelf, along with data from nearby POLENET and TAMSEIS stations to better resolve this region. Relative P wave travel time residuals from 1300 teleseismic events, obtained using a multichannel cross-correlation method, have been inverted for a seismic velocity model of the upper mantle throughout the Ross Sea Embayment. Our results suggest that the low wave speed structure under Ross Island extends roughly halfway across the Embayment and south along the Transantarctic Mountains. This observation is consistent with a two-phase rifting history for the WARS in which broad, late Cretaceous rifting between Marie Byrd Land and the TAMS transitioned to more focused rifting along the TAMS margin in the Cenozoic.

Improved Coinfection with Amphotropic Pseudotyped Retroviral Vectors

Directory of Open Access Journals (Sweden)

Yuehong Wu

2009-01-01

Full Text Available Amphotropic pseudotyped retroviral vectors have typically been used to infect target cells without prior concentration. Although this can yield high rates of infection, higher rates may be needed where highly efficient coinfection of two or more vectors is needed. In this investigation we used amphotropic retroviral vectors produced by the Plat-A cell line and studied coinfection rates using green and red fluorescent proteins (EGFP and dsRed2. Target cells were primary human fibroblasts (PHF and 3T3 cells. Unconcentrated vector preparations produced a coinfection rate of ∼4% (defined as cells that are both red and green as a percentage of all cells infected. Optimized spinoculation, comprising centrifugation at 1200 g for 2 hours at 15∘C, increased the coinfection rate to ∼10%. Concentration by centrifugation at 10,000 g or by flocculation using Polybrene increased the coinfection rate to ∼25%. Combining the two processes, concentration by Polybrene flocculation and optimized spinoculation, increased the coinfection rate to 35% (3T3 or >50% (PHF. Improved coinfection should be valuable in protocols that require high transduction by combinations of two or more retroviral vectors.

Success: Richard Dyer on Diana Ross [and Beyond

NARCIS (Netherlands)

Kooijman, J.

2016-01-01

In June 1982, film scholar Richard Dyer published a two-page essay on African-American star Diana Ross in the journal Marxism Today. Part of Dyer’s essay focuses on the American conception of success and specifically on how Ross is one of the few black artists who has been "allowed" to be such a

Leucine-rich repeat-containing G protein-coupled receptor 4 facilitates vesicular stomatitis virus infection by binding vesicular stomatitis virus glycoprotein.

Science.gov (United States)

Zhang, Na; Huang, Hongjun; Tan, Binghe; Wei, Yinglei; Xiong, Qingqing; Yan, Yan; Hou, Lili; Wu, Nannan; Siwko, Stefan; Cimarelli, Andrea; Xu, Jianrong; Han, Honghui; Qian, Min; Liu, Mingyao; Du, Bing

2017-10-06

Vesicular stomatitis virus (VSV) and rabies and Chandipura viruses belong to the Rhabdovirus family. VSV is a common laboratory virus to study viral evolution and host immune responses to viral infection, and recombinant VSV-based vectors have been widely used for viral oncolysis, vaccination, and gene therapy. Although the tropism of VSV is broad, and its envelope glycoprotein G is often used for pseudotyping other viruses, the host cellular components involved in VSV infection remain unclear. Here, we demonstrate that the host protein leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is essential for VSV and VSV-G pseudotyped lentivirus (VSVG-LV) to infect susceptible cells. Accordingly, Lgr4-deficient mice had dramatically decreased VSV levels in the olfactory bulb. Furthermore, Lgr4 knockdown in RAW 264.7 cells also significantly suppressed VSV infection, and Lgr4 overexpression in RAW 264.7 cells enhanced VSV infection. Interestingly, only VSV infection relied on Lgr4, whereas infections with Newcastle disease virus, influenza A virus (A/WSN/33), and herpes simplex virus were unaffected by Lgr4 status. Of note, assays of virus entry, cell ELISA, immunoprecipitation, and surface plasmon resonance indicated that VSV bound susceptible cells via the Lgr4 extracellular domain. Pretreating cells with an Lgr4 antibody, soluble LGR4 extracellular domain, or R-spondin 1 blocked VSV infection by competitively inhibiting VSV binding to Lgr4. Taken together, the identification of Lgr4 as a VSV-specific host factor provides important insights into understanding VSV entry and its pathogenesis and lays the foundation for VSV-based gene therapy and viral oncolytic therapeutics. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

The Rossing file

International Nuclear Information System (INIS)

Roberts, A.

1980-01-01

The subject is discussed under the headings: the issues; Rio Tinto-Zinc; Namibia; the United Nations Decree No. 1; enter RTZ; signing the contracts; Namibia's response -SWAPO; the Rossing Mine; workers' voice. It is argued that the uranium mining operations are illegal. The wages, working conditions and living standards of the workers are criticized. (U.K.)

Jimmy, Sinclair, and Jim: on the biographical trail of James Sinclair Ross Jimmy, Sinclair, and Jim: on the biographical trail of James Sinclair Ross

Directory of Open Access Journals (Sweden)

John J. O'Connor

2008-04-01

Full Text Available The deaths of Earle Birney and Robertson Davies late in 1995 reminded readers of Canadian literature that the old order—those writers born before the First World War—was quickly passing. Of the major figures born in the early years of this century (Birney, Davies, Callaghan, MacLennan, Ross, only Ross was still living at the beginning of 1996, albeit in very poor health in a nursing home in Vancouver. The deaths of Earle Birney and Robertson Davies late in 1995 reminded readers of Canadian literature that the old order—those writers born before the First World War—was quickly passing. Of the major figures born in the early years of this century (Birney, Davies, Callaghan, MacLennan, Ross, only Ross was still living at the beginning of 1996, albeit in very poor health in a nursing home in Vancouver.

Noncommutative Blackwell-Ross martingale inequality

Science.gov (United States)

Talebi, Ali; Moslehian, Mohammad Sal; Sadeghi, Ghadir

We establish a noncommutative Blackwell-Ross inequality for supermartingales under a suitable condition which generalizes Khan’s work to the noncommutative setting. We then employ it to deduce an Azuma-type inequality.

The Paediatric Cardiology Hall of Fame – Donald Nixon Ross.

Science.gov (United States)

Somerville, Jane

2015-10-01

Donald Nixon Ross, FRCS (4 October 1922 to 7 July 2014) was a South African-born British cardiothoracic surgeon, who developed the pulmonary autograft, known as the Ross procedure, for the treatment of aortic valve disease, and also performed the first heart transplant in the United Kingdom in 1968. This paper, written by Jane Somerville, Professor of Cardiology [Retired], Imperial College London, London, United Kingdom, provides the personal recollections about Donald Ross from Jane Somerville, and thus provides a unique snapshot of cardiac surgical history.

Ross-Stirling engines: Variations on a theme

Energy Technology Data Exchange (ETDEWEB)

Walker, G; Fauvel, R

1986-01-01

A new mechanism called the Ross linkage offers the prospect for compact, lightweight, long-lived, relatively low-cost, Stirling engines with excellent prospects for early commercial developed for various applications. Ross-Stirling engines are unusually compact, with installation envelope about one-third of conventional engines, piston side forces are virtually eliminated facilitating the use of dry lubricated or close tolerance, gas lubricated seals and the linkage geometry automatically favors the large bore/short ratios preferred for Stirling engines. The linkage is simple to make with few moving parts so that cost is relatively low. Various potential or actual embodiments of Ross-Stirling engines are reviewed including Stirling-Stirling gas-fired heat pumps, multicylinder power systems and cryocoolers. The system has sufficient flexibility to readily accommodate widely disparate piston stroke and cylinder diameters. Most work has been done so far with two-piston Stirling engines but the same linkage may be adopted for piston-displacer Stirling engines. 6 refs., 10 figs.

Phenolic compounds in Ross Sea water

Science.gov (United States)

Zangrando, Roberta; Barbaro, Elena; Gambaro, Andrea; Barbante, Carlo; Corami, Fabiana; Kehrwald, Natalie; Capodaglio, Gabriele

2016-04-01

Phenolic compounds are semi-volatile organic compounds produced during biomass burning and lignin degradation in water. In atmospheric and paleoclimatic ice cores studies, these compounds are used as biomarkers of wood combustion and supply information on the type of combusted biomass. Phenolic compounds are therefore indicators of paleoclimatic interest. Recent studies of Antarctic aerosols highlighted that phenolic compounds in Antarctica are not exclusively attributable to biomass burning but also derive from marine sources. In order to study the marine contribution to aerosols we developed an analytical method to determine the concentration of vanillic acid, vanillin, p-coumaric acid, syringic acid, isovanillic acid, homovanillic acid, syringaldehyde, acetosyringone and acetovanillone present in dissolved and particle phases in Sea Ross waters using HPLC-MS/MS. The analytical method was validated and used to quantify phenolic compounds in 28 sea water samples collected during a 2012 Ross Sea R/V cruise. The observed compounds were vanillic acid, vanillin, acetovanillone and p-coumaric acid with concentrations in the ng/L range. Higher concentrations of analytes were present in the dissolved phase than in the particle phase. Sample concentrations were greatest in the coastal, surficial and less saline Ross Sea waters near Victoria Land.

Radiological Operations Support Specialist (ROSS) Pilot Course Summary and Recommendations

Energy Technology Data Exchange (ETDEWEB)

Alai, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Askin, A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Buddemeier, B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wogan, L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Doshi, P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Tai, L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2016-09-30

In support of the Department of Homeland Security / Science and Technology Directorate’s (DHS/S&T) creation of a new position called the Radiological Operations Support Specialist (ROSS), Lawrence Livermore National Laboratory (LLNL) in Sub-task 1.1 and 1.2 has assisted in the development of the ROSS skills, knowledge, and abilities (SKAs); identified potentially relevant training; cross-mapped the training to the SKAs; and identified gaps in the training related to the SKAs, as well as their respective level of training knowledge - current versus desired. In the follow on task, Sub-task 1.3, a 5 day ROSS Pilot Training course was developed to fill the priority gaps identified in Sub-Task 1.2. Additionally, in Sub-Task 1.5, LLNL has performed a gap analysis of electronic tools, handbooks, and job-aides currently available to the ROSS and developed recommendations for additional and next generation tools to ensure the operational effectiveness of the ROSS position. This document summarizes the feedback received from the instructors and pilot course observers on what worked in the course and what could be improved as well as an assessment of the Pre- and Post-Test administered to the students.

Radiological Operations Support Specialist (ROSS) Pilot Course Summary and Recommendations

International Nuclear Information System (INIS)

Alai, M.; Askin, A.; Buddemeier, B.; Wogan, L.; Doshi, P.; Tai, L.

2016-01-01

In support of the Department of Homeland Security / Science and Technology Directorate's (DHS/S&T) creation of a new position called the Radiological Operations Support Specialist (ROSS), Lawrence Livermore National Laboratory (LLNL) in Sub-task 1.1 and 1.2 has assisted in the development of the ROSS skills, knowledge, and abilities (SKAs); identified potentially relevant training; cross-mapped the training to the SKAs; and identified gaps in the training related to the SKAs, as well as their respective level of training knowledge - current versus desired. In the follow on task, Sub-task 1.3, a 5 day ROSS Pilot Training course was developed to fill the priority gaps identified in Sub-Task 1.2. Additionally, in Sub-Task 1.5, LLNL has performed a gap analysis of electronic tools, handbooks, and job-aides currently available to the ROSS and developed recommendations for additional and next generation tools to ensure the operational effectiveness of the ROSS position. This document summarizes the feedback received from the instructors and pilot course observers on what worked in the course and what could be improved as well as an assessment of the Pre- and Post-Test administered to the students.

Climate Variability, Social and Environmental Factors, and Ross River Virus Transmission: Research Development and Future Research Needs

Science.gov (United States)

Tong, Shilu; Dale, Pat; Nicholls, Neville; Mackenzie, John S.; Wolff, Rodney; McMichael, Anthony J.

2008-01-01

Background Arbovirus diseases have emerged as a global public health concern. However, the impact of climatic, social, and environmental variability on the transmission of arbovirus diseases remains to be determined. Objective Our goal for this study was to provide an overview of research development and future research directions about the interrelationship between climate variability, social and environmental factors, and the transmission of Ross River virus (RRV), the most common and widespread arbovirus disease in Australia. Methods We conducted a systematic literature search on climatic, social, and environmental factors and RRV disease. Potentially relevant studies were identified from a series of electronic searches. Results The body of evidence revealed that the transmission cycles of RRV disease appear to be sensitive to climate and tidal variability. Rainfall, temperature, and high tides were among major determinants of the transmission of RRV disease at the macro level. However, the nature and magnitude of the interrelationship between climate variability, mosquito density, and the transmission of RRV disease varied with geographic area and socioenvironmental condition. Projected anthropogenic global climatic change may result in an increase in RRV infections, and the key determinants of RRV transmission we have identified here may be useful in the development of an early warning system. Conclusions The analysis indicates that there is a complex relationship between climate variability, social and environmental factors, and RRV transmission. Different strategies may be needed for the control and prevention of RRV disease at different levels. These research findings could be used as an additional tool to support decision making in disease control/surveillance and risk management. PMID:19079707

The Ross II procedure: pulmonary autograft in the mitral position.

Science.gov (United States)

Athanasiou, Thanos; Cherian, Ashok; Ross, Donald

2004-10-01

The surgical management of mitral valve disease in women of childbearing age, young patients, and children with congenital mitral valve defects is made difficult by the prospect of lifelong anticoagulation. We suggest the use of a pulmonary autograft in the mitral position (Ross II procedure) as an alternative surgical technique. We present a review of the literature, historical perspectives, indications, selection criteria, and surgical technique for the Ross II procedure. Our literature search identified 14 studies that reported results from the Ross II operation. Performed in 103 patients, the overall in-hospital mortality was 7 (6.7%), with a late mortality of 10 (9%). Although further research is needed, current evidence suggests the Ross II operation is a valuable alternative in low-risk young patients where valve durability and the complication rate from other procedures is unsatisfactory and anticoagulation not ideal.

Histomorphometry of the esophagus of adult ross broilers | Mobini ...

African Journals Online (AJOL)

The purpose of this study was to determine the histomorphometrical variations of esophagus at different regions in Ross broilers. Twenty four apparently healthy adult Ross broilers (12 females and 12 males), aged from 7 to 9 weeks, were used. Tissue samples were taken from middle parts of cervical and thoracic regions of ...

Quantifying fall migration of Ross's gulls (Rhodostethia rosea) past Point Barrow, Alaska

Science.gov (United States)

Uher-Koch, Brian D.; Davis, Shanti E.; Maftei, Mark; Gesmundo, Callie; Suydam, R.S.; Mallory, Mark L.

2014-01-01

The Ross's gull (Rhodostethia rosea) is a poorly known seabird of the circumpolar Arctic. The only place in the world where Ross's gulls are known to congregate is in the near-shore waters around Point Barrow, Alaska where they undertake an annual passage in late fall. Ross's gulls seen at Point Barrow are presumed to originate from nesting colonies in Siberia, but neither their origin nor their destination has been confirmed. Current estimates of the global population of Ross's gulls are based largely on expert opinion, and the only reliable population estimate is derived from extrapolations from previous counts conducted at Point Barrow, but these data are now over 25 years old. In order to update and clarify the status of this species in Alaska, our study quantified the timing, number, and flight direction of Ross's gulls passing Point Barrow in 2011. We recorded up to two-thirds of the estimated global population of Ross's gulls (≥ 27,000 individuals) over 39 days with numbers peaking on 16 October when we observed over 7,000 birds during a three-hour period.

Effective in vivo and ex vivo gene transfer to intestinal mucosa by VSV-G-pseudotyped lentiviral vectors

Directory of Open Access Journals (Sweden)

Kasahara Noriyuki

2010-05-01

Full Text Available Abstract Background Gene transfer to the gastrointestinal (GI mucosa is a therapeutic strategy which could prove particularly advantageous for treatment of various hereditary and acquired intestinal disorders, including inflammatory bowel disease (IBD, GI infections, and cancer. Methods We evaluated vesicular stomatitis virus glycoprotein envelope (VSV-G-pseudotyped lentiviral vectors (LV for efficacy of gene transfer to both murine rectosigmoid colon in vivo and human colon explants ex vivo. LV encoding beta-galactosidase (LV-β-Gal or firefly-luciferase (LV-fLuc reporter genes were administered by intrarectal instillation in mice, or applied topically for ex vivo transduction of human colorectal explant tissues from normal individuals. Macroscopic and histological evaluations were performed to assess any tissue damage or inflammation. Transduction efficiency and systemic biodistribution were evaluated by real-time quantitative PCR. LV-fLuc expression was evaluated by ex vivo bioluminescence imaging. LV-β-Gal expression and identity of transduced cell types were examined by histochemical and immunofluorescence staining. Results Imaging studies showed positive fLuc signals in murine distal colon; β-Gal-positive cells were found in both murine and human intestinal tissue. In the murine model, β-Gal-positive epithelial and lamina propria cells were found to express cytokeratin, CD45, and CD4. LV-transduced β-Gal-positive cells were also seen in human colorectal explants, consisting mainly of CD45, CD4, and CD11c-positive cells confined to the LP. Conclusions We have demonstrated the feasibility of LV-mediated gene transfer into colonic mucosa. We also identified differential patterns of mucosal gene transfer dependent on whether murine or human tissue was used. Within the limitations of the study, the LV did not appear to induce mucosal damage and were not distributed beyond the distal colon.

Ross Ice Shelf airstream driven by polar vortex cyclone

Science.gov (United States)

Schultz, Colin

2012-07-01

The powerful air and ocean currents that flow in and above the Southern Ocean, circling in the Southern Hemisphere's high latitudes, form a barrier to mixing between Antarctica and the rest of the planet. Particularly during the austral winter, strong westerly winds isolate the Antarctic continent from heat, energy, and mass exchange, bolstering the scale of the annual polar ozone depletion and driving the continent's record-breaking low temperatures. Pushing through this wall of high winds, the Ross Ice Shelf airstream (RAS) is responsible for a sizable amount of mass and energy exchange from the Antarctic inland areas to lower latitudes. Sitting due south of New Zealand, the roughly 470,000-square-kilometer Ross Ice Shelf is the continent's largest ice shelf and a hub of activity for Antarctic research. A highly variable lower atmospheric air current, RAS draws air from the inland Antarctic Plateau over the Ross Ice Shelf and past the Ross Sea. Drawing on modeled wind patterns for 2001-2005, Seefeldt and Cassano identify the primary drivers of RAS.

Outcomes of Reintervention on the Autograft After Ross Procedure.

Science.gov (United States)

Kumar, S Ram; Bansal, Neeraj; Wells, Winfield J; Starnes, Vaughn A

2016-11-01

After a Ross procedure, a small subset of patients requires reintervention for autograft dilatation or valve insufficiency. We sought to determine the indications, nature, and outcomes of autograft reinterventions in the left ventricular outflow tract after a Ross procedure. We retrospectively reviewed the charts of 316 consecutive patients, age 4 days to 70 years, who underwent a Ross procedure at our institution. Of these, 47 patients (15%) required autograft reintervention during a median follow-up of 8.2 years. Forty-seven patients, median age 26 years (interquartile range [IQR], 14.4 to 44.8 years), 32 (68%) men, required autograft reintervention a median 5.5 years (IQR, 1.8 to 9.4 years) after a Ross procedure. In 47% (22 of 47 patients), the autograft valve could be salvaged (15 valve-sparing aortic replacements, 7 valve repairs). The remaining 53% underwent replacement of the root (10 mechanical, 5 homograft) or valve alone (7 mechanical, 3 bioprosthetic). Twenty patients presented without autograft root dilatation 1 year (IQR, 0.5 to 2.9 years) after Ross. Fifteen of them (75%) required valve replacement. The 27 patients who demonstrated root dilatation presented 6.9 years (IQR, 4.5 to 9.7 years; p Ross, and 17 (63%) of these valves could be spared (p = 0.01). There was no surgical mortality and 5 (11%) major morbidity events. Patients were followed up for 4.9 years (IQR, 2.1 to 7 years) after left ventricular outflow tract reintervention. For patients whose autograft valve could be spared, 3-, 5-, and 8-year freedom from valve replacement was 92%, 86%, and 86%, respectively. At last follow-up, only 1 patient had greater than mild aortic insufficiency, and all but 1 had normal ejection fraction. Autograft reintervention after a Ross appears to follow a bimodal distribution. Patients with primary autograft leaflet problems tend to present early without root dilatation and frequently require valve replacement. The autograft valve can be salvaged in the

Long-term results of the Ross procedure in a population-based follow-up.

Science.gov (United States)

Kallio, Merja; Pihkala, Jaana; Sairanen, Heikki; Mattila, Ilkka

2015-05-01

The purpose of this study was to evaluate the long-term outcomes of the Ross procedure in a nationwide follow-up. This retrospective study involved all children treated with the Ross procedure in Finland between 1994 and 2009. The clinical records were reviewed for demographic and anatomical characteristics, Ross operation data, surgical history and status at the latest follow-up. The median follow-up time was 11.5 (range 2.4-19.2) years. Fifty-one patients underwent either the Ross (n = 37) or the Ross-Konno (n = 14) procedure at a median age of 4.8 (range 0.02-16.3) years, including 13 infants (Ross procedure was aortic valve stenosis, regurgitation or both, which was observed in 29, 24 and 47% of patients, respectively. The early mortality (before hospital discharge) rate was 10% (31% in infants) and the late mortality rate 6% (15% in infants). Higher mortality was discovered in patients treated with the Ross-Konno procedure (P = 0.001). The most common cause for reintervention was pulmonary homograft stenosis. The rate of freedom from right ventricular outflow tract reintervention was 98% at 5 years, 83% at 10 years and 59% at 15 years. The rate of freedom from autograft reintervention was 98% at 5 and 10 years, and 81% at 15 years. At the latest follow-up visit, mild-to-moderate aortic root dilatation was reported in 52% of patients, and 4 patients had undergone autograft-related reinterventions. Trivial autograft valve regurgitation was commonly seen, but only 1 patient developed severe autograft regurgitation requiring mechanical valve replacement 15.9 years after the Ross operation. The most common reason for reintervention after the Ross procedure in children is homograft stenosis. Aortic root dilatation and autograft valve regurgitation are relatively common but rarely lead to reinterventions before adulthood. Intraoperative complications and complex cardiac anatomy are associated with high mortality in infants undergoing the Ross-Konno procedure. In our

Rossing reaps training benefits

Energy Technology Data Exchange (ETDEWEB)

1982-06-17

A training program for unskilled operators at the Rossing Uranium Mine provides on avenue for a number of Namibians to become skilled workers without doing an apprenticeship and despite a lack of basic education. The Patterson plan has worked well so far for the purpose. A cadet scheme for leadership and trust foundation for scholarship have also been implemented.

Rossing reaps training benefits

International Nuclear Information System (INIS)

Anon.

1982-01-01

A training program for unskilled operators at the Rossing Uranium Mine provides on avenue for a number of Namibians to become skilled workers without doing an apprenticeship and despite a lack of basic education. The Patterson plan has worked well so far for the purpose. A cadet scheme for leadership and trust foundation for scholarship have also been implemented

Defining glycoprotein cancer biomarkers by MS in conjunction with glycoprotein enrichment.

Science.gov (United States)

Song, Ehwang; Mechref, Yehia

2015-01-01

Protein glycosylation is an important and common post-translational modification. More than 50% of human proteins are believed to be glycosylated to modulate the functionality of proteins. Aberrant glycosylation has been correlated to several diseases, such as inflammatory skin diseases, diabetes mellitus, cardiovascular disorders, rheumatoid arthritis, Alzheimer's and prion diseases, and cancer. Many approved cancer biomarkers are glycoproteins which are not highly abundant proteins. Therefore, effective qualitative and quantitative assessment of glycoproteins entails enrichment methods. This chapter summarizes glycoprotein enrichment methods, including lectin affinity, immunoaffinity, hydrazide chemistry, hydrophilic interaction liquid chromatography, and click chemistry. The use of these enrichment approaches in assessing the qualitative and quantitative changes of glycoproteins in different types of cancers are presented and discussed. This chapter highlights the importance of glycoprotein enrichment techniques for the identification and characterization of new reliable cancer biomarkers.

Does temperature structure phytoplankton community composition in the Ross Sea, Antarctica?

Science.gov (United States)

The Ross Sea polynya experiences one of the largest phytoplankton blooms in the Southern Ocean. Energy flow potential within the Ross Sea food web is primarily set by diatoms and prymnesiophytes, the latter dominated by Phaeocystis antarctica. We investigated physical, chemical,...

Improved Survival After the Ross Procedure Compared With Mechanical Aortic Valve Replacement.

Science.gov (United States)

Buratto, Edward; Shi, William Y; Wynne, Rochelle; Poh, Chin L; Larobina, Marco; O'Keefe, Michael; Goldblatt, John; Tatoulis, James; Skillington, Peter D

2018-03-27

It is unclear whether the Ross procedure offers superior survival compared with mechanical aortic valve replacement (AVR). This study evaluated experience and compared long-term survival between the Ross procedure and mechanical AVR. Between 1992 and 2016, a total of 392 Ross procedures were performed. These were compared with 1,928 isolated mechanical AVRs performed during the same time period as identified using the University of Melbourne and Australia and New Zealand Society of Cardiac and Thoracic Surgeons' Cardiac Surgery Databases. Only patients between 18 and 65 years of age were included. Propensity-score matching was performed for risk adjustment. Ross procedure patients were younger, and had fewer cardiovascular risk factors. The Ross procedure was associated with longer cardiopulmonary bypass and aortic cross-clamp times. Thirty-day mortality was similar (Ross, 0.3%; mechanical, 0.8%; p = 0.5). Ross procedure patients experienced superior unadjusted long-term survival at 20 years (Ross, 95%; mechanical, 68%; p mechanical, 84%; p = 0.018). In this Australian, propensity-score matched study, the Ross procedure was associated with better long-term survival compared with mechanical AVR. In younger patients, with a long life expectancy, the Ross procedure should be considered in centers with sufficient expertise. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

Validation of Robotic Surgery Simulator (RoSS).

Science.gov (United States)

Kesavadas, Thenkurussi; Stegemann, Andrew; Sathyaseelan, Gughan; Chowriappa, Ashirwad; Srimathveeravalli, Govindarajan; Seixas-Mikelus, Stéfanie; Chandrasekhar, Rameella; Wilding, Gregory; Guru, Khurshid

2011-01-01

Recent growth of daVinci Robotic Surgical System as a minimally invasive surgery tool has led to a call for better training of future surgeons. In this paper, a new virtual reality simulator, called RoSS is presented. Initial results from two studies - face and content validity, are very encouraging. 90% of the cohort of expert robotic surgeons felt that the simulator was excellent or somewhat close to the touch and feel of the daVinci console. Content validity of the simulator received 90% approval in some cases. These studies demonstrate that RoSS has the potential of becoming an important training tool for the daVinci surgical robot.

A pseudotype baculovirus expressing the capsid protein of foot-and-mouth disease virus and a T-Cell immunogen shows enhanced immunogenicity in mice

Directory of Open Access Journals (Sweden)

Liu Xiangtao

2011-02-01

Full Text Available Abstract Background Foot-and-mouth disease (FMD is a highly contagious disease of livestock which causes severe economic loss in cloven-hoofed animals. Vaccination is still a major strategy in developing countries to control FMD. Currently, inactivated vaccine of FMDV has been used in many countries with limited success and safety concerns. Development of a novel effective vaccine is must. Methods In the present study, two recombinant pseudotype baculoviruses, one expressing the capsid of foot-and-mouth disease virus (FMDV under the control of a cytomegalovirus immediate early enhancer/promoter (CMV-IE, and the other the caspid plus a T-cell immunogen coding region under a CAG promoter were constructed, and their expression was characterized in mammalian cells. In addition, their immunogenicity in a mouse model was investigated. The humoral and cell-mediated immune responses induced by pseudotype baculovirus were compared with those of inactivated vaccine. Results Indirect immunofluorescence assay (IFA and indirect sandwich-ELISA (IS-ELISA showed both recombinant baculoviruses (with or without T-cell epitopes were transduced efficiently and expressed target proteins in BHK-21 cells. In mice, intramuscular inoculation of recombinants with 1 × 109 or 1 × 1010 PFU/mouse induced the production of FMDV-specific neutralizing antibodies and gamma interferon (IFN-γ. Furthermore, recombinant baculovirus with T-cell epitopes had better immunogenicity than the recombinant without T-cell epitopes as demonstrated by significantly enhanced IFN-γ production (P P Conclusions These results indicate that pseudotype baculovirus-mediated gene delivery could be a alternative strategy to develop a new generation of vaccines against FMDV infection.

Glycoprotein on cell surfaces

International Nuclear Information System (INIS)

Muramatsu, T.

1975-01-01

There are conjugated polysaccharides in cell membranes and outside of animal cells, and they play important role in the control of cell behavior. In this paper, the studies on the glycoprotein on cell surfaces are reported. It was found that the glycoprotein on cell surfaces have both N-glycoside type and O-glycoside type saccharic chains. Therefore it can be concluded that the basic structure of the saccharic chains in the glycoprotein on cell surfaces is similar to that of blood serum and body fluid. The main glycoprotein in the membranes of red blood corpuscles has been studied most in detail, and it also has both types of saccharic chains. The glycoprotein in liver cell membranes was found to have only the saccharic chains of acid type and to be in different pattern from that in endoplasmic reticula and nuclear membranes, which also has the saccharic chains of neutral type. The structure of the saccharic chains of H-2 antigen, i.e. the peculiar glycoprotein on the surfaces of lymph system cells, has been studied, and it is similar to the saccharic chains of glycoprotein in blood serum. The saccharic chain structures of H-2 antigen and TL antigen are different. TL, H-2 (D), Lna and H-2 (K) are the glycoprotein on cell surfaces, and are independent molecules. The analysis of the saccharic chain patterns on cell surfaces was carried out, and it was shown that the acid type saccharic chains were similar to those of ordinary glycoprotein, because the enzyme of pneumococci hydrolyzed most of the acid type saccharic chains. The change of the saccharic chain patterns of glycoprotein on cell surfaces owing to canceration and multiplication is complex matter. (Kako, I.)

STS-74 M.S. Jerry L. Ross suits up

Science.gov (United States)

1995-01-01

Spaceflight veteran Jerry L. Ross, Mission Specialist 2 on Shuttle Mission STS-74, is assisted by a suit technician as he finishes getting into his launch/entry suit in the Operations and Checkout Building. Ross and four fellow astronauts will depart shortly for Launch Pad 39A, where the Space Shuttle Atlantis awaits a second liftoff attempt during a seven-minute window scheduled to open at approximately 7:30 a.m. EST, Nov. 12.

75 FR 32802 - Certificate of Alternative Compliance for the Offshore Supply Vessel ROSS CANDIES

Science.gov (United States)

2010-06-09

... Compliance for the Offshore Supply Vessel ROSS CANDIES AGENCY: Coast Guard, DHS. ACTION: Notice. SUMMARY: The... vessel ROSS CANDIES as required by 33 U.S.C. 1605(c) and 33 CFR 81.18. DATES: The Certificate of... the offshore supply vessel ROSS CANDIES, O.N. 1222260. Full compliance with 72 COLREGS [[Page 32803...

Influence of Meteorological Regimes on Cloud Microphysics Over Ross Island, Antarctica

Science.gov (United States)

Glennon, C.; Wang, S. H.; Scott, R. C.; Bromwich, D. H.; Lubin, D.

2017-12-01

The Antarctic provides a sharp contrast in cloud microphysics from the high Arctic, due to orographic lifting and resulting strong vertical motions induced by mountain ranges and other varying terrain on several spatial scales. The Atmospheric Radiation Measurement (ARM) West Antarctic Radiation Experiment (AWARE) deployed advanced cloud remote sensing equipment to Ross Island, Antarctica, from December 2015 until January 2016. This equipment included scanning and zenith radars operating in the Ka and X bands, a high spectral resolution lidar (HSRL), and a polarized micropulse lidar (MPL). A major AWARE objective is to provide state-of-the-art data for improving cloud microphysical parameterizations in climate models. To further this objective we have organized and classified the local Ross Island meteorology into distinct regimes using k-means clustering on ERA-Interim reanalysis data. We identify synoptic categories producing unique regimes of cloud cover and cloud microphysical properties over Ross Island. Each day of observations can then be associated with a specific meteorological regime, thus assisting modelers with identifying case studies. High-resolution (1 km) weather forecasts from the Antarctic Mesoscale Prediction System (AMPS) are sorted into these categories. AMPS-simulated anomalies of cloud fraction, near-surface air temperature, and vertical velocity at 500-mb are composited and compared with ground-based radar and lidar-derived cloud properties to identify mesoscale meteorological processes driving Antarctic cloud formation. Synoptic lows over the Ross and Amundsen Seas drive anomalously warm conditions at Ross Island by injecting marine air masses inland over the West Antarctic Ice Sheet (WAIS). This results in ice and mixed-phase orographic cloud systems arriving at Ross Island from the south to southeast along the Transantarctic Mountains. In contrast, blocking over the Amundsen Sea region brings classical liquid-dominated mixed-phase and

Ross, macdonald, and a theory for the dynamics and control of mosquito-transmitted pathogens.

Science.gov (United States)

Smith, David L; Battle, Katherine E; Hay, Simon I; Barker, Christopher M; Scott, Thomas W; McKenzie, F Ellis

2012-01-01

Ronald Ross and George Macdonald are credited with developing a mathematical model of mosquito-borne pathogen transmission. A systematic historical review suggests that several mathematicians and scientists contributed to development of the Ross-Macdonald model over a period of 70 years. Ross developed two different mathematical models, Macdonald a third, and various "Ross-Macdonald" mathematical models exist. Ross-Macdonald models are best defined by a consensus set of assumptions. The mathematical model is just one part of a theory for the dynamics and control of mosquito-transmitted pathogens that also includes epidemiological and entomological concepts and metrics for measuring transmission. All the basic elements of the theory had fallen into place by the end of the Global Malaria Eradication Programme (GMEP, 1955-1969) with the concept of vectorial capacity, methods for measuring key components of transmission by mosquitoes, and a quantitative theory of vector control. The Ross-Macdonald theory has since played a central role in development of research on mosquito-borne pathogen transmission and the development of strategies for mosquito-borne disease prevention.

75 FR 69432 - Ross Bachofer v. Calpine Corporation; Notice of Complaint

Science.gov (United States)

2010-11-12

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL11-3-000] Ross Bachofer v. Calpine Corporation; Notice of Complaint November 4, 2010. Take notice that on October 26, 2010, pursuant... Federal Power Act, 16 U.S.C. 2824c, Ross Bachofer (Complainant) filed a complaint against Calpine...

77 FR 56859 - Federal Register Notification of Redesignation of Potential Wilderness as Wilderness, Ross Lake...

Science.gov (United States)

2012-09-14

... Register Notification of Redesignation of Potential Wilderness as Wilderness, Ross Lake National Recreation..., Ross Lake National Recreation Area, and Lake Chelan National Recreation Area as the Stephen Mather... acres of potential wilderness within Ross Lake National Recreation Area, including approximately 1,667...

Estimating flexural rigidity and load magnitude required for formation of Ross Island flexure moat

Science.gov (United States)

Jha, S.; Harry, D. L.; Wenman, C. P.

2017-12-01

Lithospheric flexural subsidence around Ross Island in West Antarctica led to formation of the Ross Island flexure moat. This subsidence was caused by two major volcanic phases on Ross Island. The first phase saw the first surficial expression of Ross Island and volcanism at Mt. Bird to the north of Ross Island, which lasted from 5.2 - 2.9 Ma. The second phase lasted from 1.78 Ma to present and is comprised of eruptions from Mt. Terror to the east, Mt. Erebus to the west and Hut Point Peninsula (HPP) to the south of Ross Island. Flexural subsidence of the lithosphere due to volcanism on Ross Island led to formation of a sedimentary moat around the island, which is preserved in stratigraphy imaged on seismic reflection profiles. We identified 5 unconformities (from deepest upward Ri, RMU1, RMU2, RMU3, RMU4) in the seismic surveys which correspond to flexural subsidence episodes around Ross Island since early Pliocene. Ri (4.4 Ma) lies near the bottom of the flexural moat and RMU4 near the seafloor and top of the moat fill. These unconformities were used to make isopach maps to constrain flexure modeling of the area. Isopach maps show circular or semi-circular flexure basins around Ross Island which is approximated using a continuous plate, point load flexure model. We used Ri - sea floor isopach to constrain flexure models for 5 profiles centered on 4 volcanic centers and trending radially out of Ross Island. Flexure models along two profiles beginning on Mt. Bird and one profile off HPP show a flexural rigidity range of 1.47 - 6.44 x 1018 Nm with load center of mass on Mt. Bird and on HPP, respectively. A similar model along a profile initiating on Mt. Terror, passing through Mt Erebus and extending west of Ross Island across the moat, yielded a higher flexural rigidity estimate of 2.03 x 1019 Nm with load centered at Mt. Erebus. A flexure model to the north east of Ross Island along a profile beginning at Mt Terror and trending north, provide the highest flexural

Long-Term Outcomes of the Ross Procedure Versus Mechanical Aortic Valve Replacement: Propensity-Matched Cohort Study.

Science.gov (United States)

Mazine, Amine; David, Tirone E; Rao, Vivek; Hickey, Edward J; Christie, Shakira; Manlhiot, Cedric; Ouzounian, Maral

2016-08-23

The ideal aortic valve substitute in young and middle-aged adults remains unknown. We sought to compare the long-term outcomes of patients undergoing the Ross procedure and those receiving a mechanical aortic valve replacement (AVR). From 1990 to 2014, 258 patients underwent a Ross procedure and 1444 had a mechanical AVR at a single institution. Patients were matched into 208 pairs through the use of a propensity score. Mean age was 37.2±10.2 years, and 63% were male. Mean follow-up was 14.2±6.5 years. Overall survival was equivalent (Ross versus AVR: hazard ratio, 0.91, 95% confidence interval, 0.38-2.16; P=0.83), although freedom from cardiac- and valve-related mortality was improved in the Ross group (Ross versus AVR: hazard ratio, 0.22; 95% confidence interval, 0.034-0.86; P=0.03). Freedom from reintervention was equivalent after both procedures (Ross versus AVR: hazard ratio, 1.86; 95% confidence interval, 0.76-4.94; P=0.18). Long-term freedom from stroke or major bleeding was superior after the Ross procedure (Ross versus AVR: hazard ratio, 0.09; 95% confidence interval, 0.02-0.31; PRoss procedure and mechanical AVR. However, the Ross procedure was associated with improved freedom from cardiac- and valve-related mortality and a significant reduction in the incidence of stroke and major bleeding. In specialized centers, the Ross procedure represents an excellent option and should be considered for young and middle-aged adults undergoing AVR. © 2016 American Heart Association, Inc.

76 FR 45543 - Notice of Intent To Prepare a Draft Environmental Impact Statement for the Skagit River General...

Science.gov (United States)

2011-07-29

... proposed flood-risk management project in the Skagit River Basin from Ross Dam to the river mouth at Skagit... share of the project costs. This is a single-purpose flood-risk management study. The goal of this project is to identify the National Economic Development (NED) plan, the flood-risk management alternative...

STS-88 Crew Interview: Jerry Ross

Science.gov (United States)

1998-01-01

Jerry Ross discusses the seven-day mission that will be highlighted by the mating of the U.S.-built Node 1 station element to the Functional Energy Block (FGB) which will already be in orbit, and two spacewalks to connect power and data transmission cables between the Node and the FGB. Node 1 will be the first Space Station hardware delivered by the Space Shuttle. He also disscusses the assembly sequence. The crew will conduct a series of rendezvous maneuvers similar to those conducted on other Shuttle missions to reach the orbiting FGB. Once the two elements are docked, Ross and Newman will conduct two scheduled spacewalks to connect power and data cables between the Node, PMAs and the FGB. The day following the spacewalks, Endeavour will undock from the two components, completing the first Space Station assembly mission.

California serogroup GC (G1) glycoprotein is the principal determinant of pH-dependent cell fusion and entry

International Nuclear Information System (INIS)

Plassmeyer, Matthew L.; Soldan, Samantha S.; Stachelek, Karen M.; Martin-Garcia, Julio; Gonzalez-Scarano, Francisco

2005-01-01

Members of the California serogroup of orthobunyaviruses, particularly La Crosse (LAC) and Tahyna (TAH) viruses, are significant human pathogens in areas where their mosquito vectors are endemic. Previous studies using wild-type LAC and TAH181/57, a highly neurovirulent strain with low neuroinvasiveness (Janssen, R., Gonzalez-Scarano, F., Nathanson, N., 1984. Mechanisms of bunyavirus virulence. Comparative pathogenesis of a virulent strain of La Crosse and an avirulent strain of Tahyna virus. Lab. Invest. 50 (4), 447-455), have demonstrated that the neuroinvasive phenotype maps to the M segment, the segment that encodes the two viral glycoproteins GN (G2) and GC (G1), as well as a non-structural protein NSm. To further define the role of GN and GC in fusion and entry, we prepared a panel of recombinant M segment constructs using LAC, TAH181/57, and V22F, a monoclonal-resistant variant of LAC with deficient fusion function. These M segment constructs were then tested in two surrogate assays for virus entry: a cell-to-cell fusion assay based on T7-luciferase expression, and a pseudotype transduction assay based on the incorporation of the bunyavirus glycoproteins on an MLV backbone. Both assays demonstrated that GC is the principal determinant of virus fusion and cell entry, and furthermore that the region delineated by amino acids 860-1442, corresponding to the membrane proximal two-thirds of GC, is key to these processes. These results, coupled with structural modeling suggesting homologies between the carboxy region of GC and Sindbis virus E1, suggest that the LAC GC functions as a type II fusion protein

Rossing - uranium changes the face of the Namib

International Nuclear Information System (INIS)

Anon.

1981-01-01

Rossing is not only an important source of uranium for industrial countries, but a large revenue-earner. It is injecting money into the economy. For efficiency, personnel and equipment had to be comfortably established. The Rossing orebody is the largest known deposit of uranium occuring in granite. Over one million ton of ore and waste are removed weekly. By the use of scintillometer readings the ore is blended to give a constant feed to the mill. Modern developments are used to optimise the mining operation. Radioactivity will be always present and is therefore regulary measured. The best working environment are always ensured

Does mosquito control have an effect on mosquito-borne disease? The case of Ross River virus disease and mosquito management in Queensland, Australia.

Science.gov (United States)

Tomerini, Deanna M; Dale, Pat E; Sipe, Neil

2011-03-01

We examined the relationship between types of mosquito control programs and the mosquito-borne Ross River virus (RRV) disease in Queensland, Australia. Mosquito control information was collected through a survey of the responsible agencies (local governments), and RRV disease notification data were provided by the Queensland state health authority. The study developed a typology of mosquito control programs, based on the approaches used. Based on the analysis of data on RRV disease rates between mosquito control types within 4 climatic regions, each region had different combinations of mosquito control strategies in their programs; there were also general similarities in the relationship between program types and RRV rates between the regions. The long-term RRV disease rates were lower in areas where the mosquito control program included pre-emptive (rather than reactive) surveillance based on an extensive (rather than incomplete) knowledge of mosquito habitats, and where treatment of both saltwater and freshwater habitats (compared to only saltwater habitats, in coastal areas) occurred. The data indicate that mosquito control is an effective public health intervention to reduce mosquito-borne disease; hence, climate change adaptation strategies should ensure that adequate resources are available for effective vector control so as to manage the risk of mosquito-borne diseases.

Coastal-change and glaciological map of the Ross Island area, Antarctica

Science.gov (United States)

Ferrigno, Jane G.; Foley, Kevin M.; Swithinbank, Charles; Williams, Richard S.

2010-01-01

Reduction in the area and volume of Earth?s two polar ice sheets is intricately linked to changes in global climate and to the resulting rise in sea level. Measurement of changes in area and mass balance of the Antarctic ice sheet was given a very high priority in recommendations by the Polar Research Board of the National Research Council. On the basis of these recommendations, the U.S. Geological Survey used its archive of satellite images to document changes in the cryospheric coastline of Antarctica and analyze the glaciological features of the coastal regions. The Ross Island area map is bounded by long 141? E. and 175? E. and by lat 76? S. and 81? S. The map covers the part of southern Victoria Land that includes the northwestern Ross Ice Shelf, the McMurdo Ice Shelf, part of the polar plateau and Transantarctic Mountains, the McMurdo Dry Valleys, northernmost Shackleton Coast, Hillary Coast, the southern part of Scott Coast, and Ross Island. Little noticeable change has occurred in the ice fronts on the map, so the focus is on glaciological features. In the western part of the map area, the polar plateau of East Antarctica, once thought to be a featureless region, has subtle wavelike surface forms (megadunes) and flow traces of glaciers that originate far inland and extend to the coast or into the Ross Ice Shelf. There are numerous outlet glaciers. Glaciers drain into the McMurdo Dry Valleys, through the Transantarctic Mountains into the Ross Sea, or into the Ross Ice Shelf. Byrd Glacier is the largest. West of the Transantarctic Mountains are areas of blue ice, readily identifiable on Landsat images, that have been determined to be prime areas for finding meteorites. Three subglacial lakes have been identified in the map area. Because McMurdo Station, the main U.S. scientific research station in Antarctica, is located on Ross Island in the map area, many of these and other features in the area have been studied extensively. The paper version of this map is

Aortic Valve Replacement and the Ross Operation in Children and Young Adults.

Science.gov (United States)

Sharabiani, Mansour T A; Dorobantu, Dan M; Mahani, Alireza S; Turner, Mark; Peter Tometzki, Andrew J; Angelini, Gianni D; Parry, Andrew J; Caputo, Massimo; Stoica, Serban C

2016-06-21

There are several options available for aortic valve replacement (AVR), with few comparative reports in the literature. The optimal choice for AVR in each age group is not clear. The study sought to report and compare outcomes after AVR in the young using data from a national database. AVR procedures were compared after advanced matching, both in pairs and in a 3-way manner, using a Bayesian dynamic survival model. A total of 1,501 patients who underwent AVR in the United Kingdom between 2000 and 2012 were included. Of these, 47.8% had a Ross procedure, 37.8% a mechanical AVR, 10.9% a bioprosthesis AVR, and 3.5% a homograft AVR, with Ross patients being significantly younger when compared to the other groups. Overall survival at 12 years was 94.6%. In children, the Ross procedure had a 12.7% higher event-free probability (death or any reintervention) at 10 years when compared to mechanical AVR (p = 0.05). We also compared all procedures except the homograft in a matched population of young adults, where the bioprosthesis had the lowest event-free probability of 78.8%, followed by comparable results in mechanical AVR and Ross, with 86.3% and 89.6%, respectively. Younger age was associated with mortality and pulmonary reintervention in the Ross group and with aortic reintervention in the mechanical AVR. Of all 3 options, only the patients undergoing the Ross procedure approached the survival of the general population. AVR in the young achieves good results, with the Ross being overall better suited for this age group, especially in children. Although freedom from aortic valve reintervention is superior after the Ross procedure, the need for homograft reinterventions is an issue to take into account. All methods have advantages and limitations, with reinterventions being an issue in the long term for all, more crucially in smaller children. Copyright © 2016 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.

Variability and Trends in Sea Ice Extent and Ice Production in the Ross Sea

Science.gov (United States)

Comiso, Josefino; Kwok, Ronald; Martin, Seelye; Gordon, Arnold L.

2011-01-01

Salt release during sea ice formation in the Ross Sea coastal regions is regarded as a primary forcing for the regional generation of Antarctic Bottom Water. Passive microwave data from November 1978 through 2008 are used to examine the detailed seasonal and interannual characteristics of the sea ice cover of the Ross Sea and the adjacent Bellingshausen and Amundsen seas. For this period the sea ice extent in the Ross Sea shows the greatest increase of all the Antarctic seas. Variability in the ice cover in these regions is linked to changes in the Southern Annular Mode and secondarily to the Antarctic Circumpolar Wave. Over the Ross Sea shelf, analysis of sea ice drift data from 1992 to 2008 yields a positive rate of increase in the net ice export of about 30,000 sq km/yr. For a characteristic ice thickness of 0.6 m, this yields a volume transport of about 20 cu km/yr, which is almost identical, within error bars, to our estimate of the trend in ice production. The increase in brine rejection in the Ross Shelf Polynya associated with the estimated increase with the ice production, however, is not consistent with the reported Ross Sea salinity decrease. The locally generated sea ice enhancement of Ross Sea salinity may be offset by an increase of relatively low salinity of the water advected into the region from the Amundsen Sea, a consequence of increased precipitation and regional glacial ice melt.

Ross sea ice motion, area flux, and deformation

Science.gov (United States)

kwok, Ron

2005-01-01

The sea ice motion, area export, and deformation of the Ross Sea ice cover are examined with satellite passive microwave and RADARSAT observations. The record of high-resolution synthetic aperture radar (SAR) data, from 1998 and 2000, allows the estimation of the variability of ice deformation at the small scale (10 km) and to assess the quality of the longer record of passive microwave ice motion. Daily and subdaily deformation fields and RADARSAT imagery highlight the variability of motion and deformation in the Ross Sea. With the passive microwave ice motion, the area export at a flux gate positioned between Cape Adare and Land Bay is estimated. Between 1992 and 2003, a positive trend can be seen in the winter (March-November) ice area flux that has a mean of 990 x 103 km2 and ranges from a low of 600 x 103 km2 in 1992 to a peak of 1600 x 103 km2 in 2001. In the mean, the southern Ross Sea produces almost twice its own area of sea ice during the winter. Cross-gate sea level pressure (SLP) gradients explain 60% of the variance in the ice area flux. A positive trend in this gradient, from reanalysis products, suggests a 'spinup' of the Ross Sea Gyre over the past 12 yr. In both the NCEP-NCAR and ERA-40 surface pressure fields, longer-term trends in this gradient and mean SLP between 1979 and 2002 are explored along with positive anomalies in the monthly cross-gate SLP gradient associated with the positive phase of the Southern Hemisphere annular mode and the extrapolar Southern Oscillation.

The Role of Conserved N-Linked Glycans on Ebola Virus Glycoprotein 2.

Science.gov (United States)

Lennemann, Nicholas J; Walkner, Madeline; Berkebile, Abigail R; Patel, Neil; Maury, Wendy

2015-10-01

N-linked glycosylation is a common posttranslational modification found on viral glycoproteins (GPs) and involved in promoting expression, cellular attachment, protection from proteases, and antibody evasion. The GP subunit GP2 of filoviruses contains 2 completely conserved N-linked glycosylation sites (NGSs) at N563 and N618, suggesting that they have been maintained through selective pressures. We assessed mutants lacking these glycans for expression and function to understand the role of these sites during Ebola virus entry. Elimination of either GP2 glycan individually had a modest effect on GP expression and no impact on antibody neutralization of vesicular stomatitis virus pseudotyped with Ebola virus GP. However, loss of the N563 glycan enhanced entry by 2-fold and eliminated GP detection by a well-characterized monoclonal antibody KZ52. Loss of both sites dramatically decreased GP expression and abolished entry. Surprisingly, a GP that retained a single NGS at N563, eliminating the remaining 16 NGSs from GP1 and GP2, had detectable expression, a modest increase in entry, and pronounced sensitivity to antibody neutralization. Our findings support the importance of the GP2 glycans in GP expression/structure, transduction efficiency, and antibody neutralization, particularly when N-linked glycans are also removed from GP1. © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Against Raising Hope of Raising the Dead: Contra Moody and Kubler-Ross.

Science.gov (United States)

Vicchio, Stephen J.; And Others

1979-01-01

Kubler-Ross and Moody have made assertions about survival after death. They argued that the subjects were not dead, but in the process of dying. An alternative explanation to this "glimpse of the afterlife" approach is offered. Other theological objections are raised to the Moody/Kubler-Ross approach. (Author)

78 FR 19330 - Supplemental Environmental Impact Statement for the Ross In-Situ Uranium Recovery Project in...

Science.gov (United States)

2013-03-29

... Ross In-Situ Uranium Recovery Project in Crook County, Wyoming AGENCY: Nuclear Regulatory Commission... Commission (NRC) for a new source materials license for the proposed Ross In-Situ Uranium Recovery (ISR) Project (Ross Project) proposed to be located in Crook County, Wyoming. The NRC is issuing for public...

Climatology and decadal variability of the Ross Sea shelf waters

Directory of Open Access Journals (Sweden)

A. Russo

2011-06-01

Full Text Available The World Ocean Database 2001 data located in the Ross Sea (named WOD01 and containing data in this region since 1928 are merged with recent data collected by the Italian expeditions (CLIMA dataset in the period November 1994-February 2004 in the same area. From this extended dataset, austral summer climatologies of the main Ross Sea subsurface, intermediate and bottom water masses: High Salinity Shelf Water (HSSW, Low Salinity Shelf Water (LSSW, Ice Shelf Water (ISW and Modified Circumpolar Deep Water (MCDW have been drawn. The comparison between the WOD01_1994 climatologies (a subset of the WOD01 dataset until April 1994 and the CLIMA ones for the period 1994/95-2003/04 showed significant changes occurred during the decade. The freshening of the Ross Sea shelf waters which occurred during the period 1960-2000, was confirmed by our analysis in all the main water masses, even though with a spatially varying intensity. Relevant variations were found for the MCDW masses, which appeared to reduce their presence and to deepen; this can be ascribed to the very limited freshening of the MCDW core, which allowed an increased density with respect to the surrounding waters. Variations in the MCDW properties and extension could have relevant consequences, e.g. a decreased Ross Ice Shelf basal melting or a reduced supply of nutrients, and may also be indicative of a reduced thermohaline circulation within the Ross Sea. Shelf Waters (SW having neutral density γn > 28.7 Kg m-3, which contribute to form the densest Antarctic Bottom Waters (AABW, showed a large volumetric decrease in the 1994/95-2003/04 decade, most likely as a consequence of the SW freshening.

Strontium isotopic study of sediment from the Ross Sea

International Nuclear Information System (INIS)

Kovach, J.; Faure, G.

1977-01-01

A preliminary report summarizing the results of a study of the strontium-87/strontium-86 ratios and of the rubidium and strontium concentrations of the fine-grained (less than 150 microns) noncarbonate fractions of sediment samples from core E32-25 raised from a depth of 327 fathoms in the Ross Sea at 78 0 31.0'S 164 0 24.7'W was presented. The strontium-87/strontium-86 ratios of the samples analyzed range from 0.7119 to 0.7220. Rubidium and strontium concentrations range, respectively, from 126 to 164 parts per million and from 113 to 174 parts per million. The observed strontium-87/strontium-86 ratios and strontium concentrations in sediment samples from core E32-25 fit a hyperbolic curve. These ratios can be used to estimate the concentrations of volcanogenic detritus in the sediment samples. The results of this study provide additional baseline data for anticipated future studies of sediment cores to be recovered from beneath the Ross Ice Shelf by the Ross Ice Shelf Project. 1 figure

Clinical Outcomes Following the Ross Procedure in Adults: A 25-Year Longitudinal Study.

Science.gov (United States)

Martin, Elisabeth; Mohammadi, Siamak; Jacques, Frederic; Kalavrouziotis, Dimitri; Voisine, Pierre; Doyle, Daniel; Perron, Jean

2017-10-10

Very few reports of long-term outcomes of patients who underwent the Ross procedure have been published. The authors reviewed their 25-year experience with the Ross procedure with the aim of defining very-long-term survival and factors associated with Ross-related failure. Between January 1990 and December 2014, the Ross procedure was performed in 310 adults (mean age 40.8 years) at a single institution. All patients were prospectively added to a dedicated cardiac surgery registry. Complete post-operative clinical examination and history were obtained, and transthoracic echocardiography was performed according to a standardized protocol. There was no loss to follow-up. Median follow-up was 15.1 years and up to 25 years. Bicuspid aortic valve was diagnosed in 227 patients (73.2%), and the most common indication for surgery was aortic stenosis (n = 225 [72.6%]). Freedom from any Ross-related reintervention was 92.9% and 70.1% at 10 and 20 years, respectively. Independent risk factors for pulmonary autograft degeneration were pre-operative large aortic annulus (hazard ratio: 1.1; p = 0.01), pre-operative aortic insufficiency (hazard ratio: 2.7; p = 0.002), and concomitant replacement of the ascending aorta (hazard ratio: 7.7; p = 0.0003). There were 4 hospital deaths (1.3%), and overall survival at 10 and 20 years was 94.1% and 83.6%, respectively. Long-term survival was not significantly different in patients who required Ross-related reintervention (log-rank p = 0.70). However, compared with the general population, survival was significantly lower in patients following the Ross procedure when matched on age and sex (p Ross procedure was associated with excellent long-term valvular outcomes and survival, regardless of the need for reintervention. Adults presenting with aortic insufficiency or a dilated aortic annulus or ascending aorta were at greater risk for reintervention. Unlike previous reports, long-term survival was lower in Ross

Cell-type-specific gene delivery into neuronal cells in vitro and in vivo

International Nuclear Information System (INIS)

Parveen, Zahida; Mukhtar, Muhammad; Rafi, Mohammed; Wenger, David A.; Siddiqui, Khwaja M.; Siler, Catherine A.; Dietzschold, Bernhard; Pomerantz, Roger J.; Schnell, Matthias J.; Dornburg, Ralph

2003-01-01

The avian retroviruses reticuloendotheliosis virus strain A (REV-A) and spleen necrosis virus (SNV) are not naturally infectious in human cells. However, REV-A-derived viral vectors efficiently infect human cells when they are pseudotyped with envelope proteins displaying targeting ligands specific for human cell-surface receptors. Here we report that vectors containing the gag region of REV-A and pol of SNV can be pseudotyped with the envelope protein of vesicular stomatitis virus (VSV) and the glycoproteins of different rabies virus (RV) strains. Vectors pseudotyped with the envelope protein of the highly neurotropic RV strain CVS-N2c facilitated cell type-specific gene delivery into mouse and human neurons, but did not infect other human cell types. Moreover, when such vector particles were injected into the brain of newborn mice, only neuronal cells were infected in vivo. Cell-type-specific gene delivery into neurons may present quite specific gene therapy approaches for many degenerative diseases of the brain

78 FR 12357 - Brockway Mould, Inc., a Division of Ross International Ltd. Including Robert Lerch From BJR...

Science.gov (United States)

2013-02-22

... Division of Ross International Ltd. Including Robert Lerch From BJR Trucking, Brockport, PA; Amended..., applicable to workers and former workers of Brockway Mould, Inc., a division of Ross International Ltd... Brockway Mould, Inc., a division of Ross International Ltd., including Robert Lerch from BJR Trucking...

Hemodynamic outcomes of the Ross procedure versus other aortic valve replacement: a systematic review and meta-analysis.

Science.gov (United States)

Um, Kevin J; McCLURE, Graham R; Belley-Cote, Emilie P; Gupta, Saurabh; Bouhout, Ismail; Lortie, Hugo; Alraddadi, Hatim; Alsagheir, Ali; Bossard, Matthias; McINTYRE, William F; Lengyel, Alexandra; Eikelboom, John W; Ouzounian, Maral; Chu, Michael W; Parry, Dominic; El-Hamamsy, Ismail; Whitlock, Richard P

2018-01-09

Life expectancy in young adults undergoing mechanical or bioprosthetic aortic valve replacement (AVR) may be reduced by up to 20 years compared to age matched controls. The Ross procedure is a durable, anticoagulation-sparing alternative. We performed a systematic review and meta-analysis to compare the valve hemodynamics of the Ross procedure versus other AVR. We searched Cochrane CENTRAL, MEDLINE and EMBASE from inception to February 2017 for randomized controlled trials (RCTs) and observational studies (n≥10 Ross). Independently and in duplicate, we performed title and abstract screening, full-text eligibility assessment, and data collection. We evaluated the risk of bias with the Cochrane and CLARITY tools, and the quality of evidence with the GRADE framework. We identified 2 RCTs and 13 observational studies that met eligibility criteria (n=1,412). In observational studies, the Ross procedure was associated with a lower mean aortic gradient at discharge (MD -9 mmHg, 95% CI [-13, -5], pRoss procedure was associated with a lower mean gradient at latest follow-up (MD -15 mmHg, 95% CI [-32, 2], p=0.08, I2=99%). The mean pulmonic gradient for the Ross procedure was 18.0 mmHg (95% CI [16, 20], pRoss procedure was associated with better aortic valve hemodynamics. Future studies should evaluate the impact of the Ross procedure on exercise capacity and quality of life.

Long-term Evaluation of the Ross Procedure in Acute Infective Endocarditis.

Science.gov (United States)

Ratschiller, Thomas; Sames-Dolzer, Eva; Paulus, Patrick; Schimetta, Wolfgang; Müller, Hannes; Zierer, Andreas F; Mair, Rudolf

2017-10-05

Optimal valve substitute for young patients with aortic valve endocarditis remains controversial. Given its better resistance to infection, the Ross procedure is an attractive alternative to prosthetic valve replacement or homograft implantation. The objective of this study was to assess long-term outcomes of the Ross procedure in this indication. From January 1991 to April 2017, 190 patients underwent a Ross procedure at our institution. Acute endocarditis was the indication for operation in 19 patients, including 6 patients with a bicuspid aortic valve. The pulmonary autograft was implanted as freestanding root replacement in all patients. The clinical follow-up is 100% complete, with a mean of 12.0 ± 5.7 years. The mean age of the study population was 35.9 ± 11.5 years. Moderate or severe aortic regurgitation was present in 84.2% of the patients. Systemic embolization had occurred in 36.8% of the patients. The mean aortic cross-clamp time was 126 ± 24 minutes. The median length of stay on the intensive care unit was 1 day. Mortality at 30 days was 5.3% (1 patient with gastrointestinal bleeding). Echocardiography at hospital discharge documented no or trivial aortic regurgitation in all patients. No case of recurrent endocarditis affecting the autograft occurred. One patient (0.4% per patient-year) was reoperated 1.8 years after the Ross procedure for homograft endocarditis. Three patients (15.8%) were reoperated for autograft aneurysm. The Ross procedure is a safe and effective alternative to prosthetic valve replacement or homograft implantation in selected young patients with acute endocarditis with a low rate of recurrent infection. Copyright © 2017 Elsevier Inc. All rights reserved.

In vitro comparison of three common essential oils mosquito repellents as inhibitors of the Ross River virus

Science.gov (United States)

Ralambondrainy, Miora; Belarbi, Essia; Viranaicken, Wildriss; Baranauskienė, Renata; Venskutonis, Petras Rimantas; Desprès, Philippe; El Kalamouni, Chaker; Sélambarom, Jimmy

2018-01-01

Background The essential oils of Cymbopogon citratus (CC), Pelargonium graveolens (PG) and Vetiveria zizanioides (VZ) are commonly used topically to prevent mosquito bites and thus the risk of infection by their vectored pathogens such as arboviruses. However, since mosquito bites are not fully prevented, the effect of these products on the level of viral infection remains unknown. Objectives To evaluate in vitro the essentials oils from Reunion Island against one archetypal arbovirus, the Ross River virus (RRV), and investigate the viral cycle step that was impaired by these oils. Methods The essential oils were extracted by hydrodistillation and analyzed by a combination of GC-FID and GC×GC-TOF MS techniques. In vitro studies were performed on HEK293T cells to determine their cytotoxicity, their cytoprotective and virucidal capacities on RRV-T48 strain, and the level of their inhibitory effect on the viral replication and residual infectivity prior, during or following viral adsorption using the reporter virus RRV-renLuc. Results Each essential oil was characterized by an accurate quantification of their terpenoid content. PG yielded the least-toxic extract (CC50 > 1000 μg.mL-1). For the RRV-T48 strain, the monoterpene-rich CC and PG essential oils reduced the cytopathic effect but did not display virucidal activity. The time-of-addition assay using the gene reporter RRV-renLuc showed that the CC and PG essential oils significantly reduced viral replication and infectivity when applied prior, during and early after viral adsorption. Overall, no significant effect was observed for the low monoterpene-containing VZ essential oil. Conclusion The inhibitory profiles of the three essential oils suggest the high value of the monoterpene-rich essential oils from CC and PG against RRV infection. Combined with their repellent activity, the antiviral activity of the essential oils of CC and PG may provide a new option to control arboviral infection. PMID:29771946

Recent Progress in Electrochemical Biosensors for Glycoproteins

Directory of Open Access Journals (Sweden)

Uichi Akiba

2016-12-01

Full Text Available This review provides an overview of recent progress in the development of electrochemical biosensors for glycoproteins. Electrochemical glycoprotein sensors are constructed by combining metal and carbon electrodes with glycoprotein-selective binding elements including antibodies, lectin, phenylboronic acid and molecularly imprinted polymers. A recent trend in the preparation of glycoprotein sensors is the successful use of nanomaterials such as graphene, carbon nanotube, and metal nanoparticles. These nanomaterials are extremely useful for improving the sensitivity of glycoprotein sensors. This review focuses mainly on the protocols for the preparation of glycoprotein sensors and the materials used. Recent improvements in glycoprotein sensors are discussed by grouping the sensors into several categories based on the materials used as recognition elements.

An optimal ross filter system for soft X-ray spectra measurement

International Nuclear Information System (INIS)

Huang Tianxuan; Zheng Zhijian; Sun Kexu; Jiang Shaoen

2000-01-01

A broadband Ross filter spectrometer is described for measuring soft X-ray radiation (0.1∼1.5 keV) emitted from laser plasma. It consists of a number of channels, each representing a Ross filter pair in conjunction with Al cathode X-ray diodes. An optimal channel has flat response within the sensitivity band, and minimal response outside it. The effect of some uncertainties on the accuracy of measurements is calculated

Minevikupärandit Häädemeestelt / Eevi Ross

Index Scriptorium Estoniae

Ross, Eevi, 1938-

2012-01-01

Raamatututvustus: Minevikupärandit Häädemeestelt : valimik korrespondentide murdetekste. VIII / Eesti Teaduste Akadeemia Emakeele Selts ; kogunud Marta Mäesalu ; koostanud ja toimetanud: Helju Kaal, Eevi Ross. Tallinn : Emakeele Selts, 2012

Sir Ronald Ross and the Malarial Parasite

Indian Academy of Sciences (India)

Home; Journals; Resonance – Journal of Science Education; Volume 11; Issue 7. Sir Ronald Ross and the Malarial Parasite - Discovery of its Route - From Man to Mosquito and Back. Shobhona Sharma. General Article Volume 11 Issue 7 July 2006 pp 4-13 ...

The Ross procedure offers excellent survival compared with mechanical aortic valve replacement in a real-world setting.

Science.gov (United States)

Andreas, Martin; Wiedemann, Dominik; Seebacher, Gernot; Rath, Claus; Aref, Tandis; Rosenhek, Raphael; Heinze, Georg; Eigenbauer, Ernst; Simon, Paul; Ruetzler, Kurt; Hiesmayr, Joerg-Michael; Moritz, Anton; Laufer, Guenther; Kocher, Alfred

2014-09-01

The ideal prosthesis for young patients requiring aortic valve replacement has not been defined to date. Although the Ross procedure provides excellent survival, its application is still limited. We compared the long-term survival after the Ross procedure with mechanical aortic valve replacement. All consecutive Ross procedures and mechanical aortic valve replacements performed between 1991 and 2008 at a single centre were analysed. Only adult patients between 18 and 50 years of age were included in the study. Survival and valve-related complications were evaluated. Furthermore, survival was compared with the age- and sex-matched Austrian population. A total of 159 Ross patients and 173 mechanical valve patients were included. The cumulative survival for the Ross procedure was significantly better, with survival rates of 96, 94 and 93% at 5, 10 and 15 years, respectively, in comparison to 90, 84 and 75% (P Ross group but was significantly reduced in the mechanical valve group. In a real-world setting, the Ross procedure is associated with a long-term survival benefit in young adults in comparison to mechanical aortic valve replacement. © The Author 2014. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

Development of glycoprotein capture-based label-free method for the high-throughput screening of differential glycoproteins in hepatocellular carcinoma.

Science.gov (United States)

Chen, Rui; Tan, Yexiong; Wang, Min; Wang, Fangjun; Yao, Zhenzhen; Dong, Liwei; Ye, Mingliang; Wang, Hongyang; Zou, Hanfa

2011-07-01

A robust, reproducible, and high throughput method was developed for the relative quantitative analysis of glycoprotein abundances in human serum. Instead of quantifying glycoproteins by glycopeptides in conventional quantitative glycoproteomics, glycoproteins were quantified by nonglycosylated peptides derived from the glycoprotein digest, which consists of the capture of glycoproteins in serum samples and the release of nonglycopeptides by trypsin digestion of captured glycoproteins followed by two-dimensional liquid chromatography-tandem MS analysis of released peptides. Protein quantification was achieved by comparing the spectrum counts of identified nonglycosylated peptides of glycoproteins between different samples. This method was demonstrated to have almost the same specificity and sensitivity in glycoproteins quantification as capture at glycopeptides level. The differential abundance of proteins present at as low as nanogram per milliliter levels was quantified with high confidence. The established method was applied to the analysis of human serum samples from healthy people and patients with hepatocellular carcinoma (HCC) to screen differential glycoproteins in HCC. Thirty eight glycoproteins were found with substantial concentration changes between normal and HCC serum samples, including α-fetoprotein, the only clinically used marker for HCC diagnosis. The abundance changes of three glycoproteins, i.e. galectin-3 binding protein, insulin-like growth factor binding protein 3, and thrombospondin 1, which were associated with the development of HCC, were further confirmed by enzyme-linked immunosorbent assay. In conclusion, the developed method was an effective approach to quantitatively analyze glycoproteins in human serum and could be further applied in the biomarker discovery for HCC and other cancers.

Downslope flow across the Ross Sea shelf break (Antarctica)

Science.gov (United States)

Bergamasco, A.; Budillon, G.; Carniel, S.; Defendi, V.; Meloni, R.; Paschini, E.; Sclavo, M.; Spezie, G.

2003-12-01

The analysis of some high-resolution hydrological data sets acquired during the 1997, 1998, 2001 and 2003 austral summers across the Ross Sea continental shelf break are here presented. The main focus of these cruises carried out in the framework of the Italian National Antarctic Program was the investigation of the downslope flow of the dense waters originated inside the Ross Sea. Such dense waters, flow near the bottom and, reaching the continental shelf break, ventilate the deep ocean. Two Antarctic continental shelf mechanisms can originate dense and deep waters. The former mechanism involves the formation, along the Victoria Land coasts, of a dense and saline water mass, the High Salinity Shelf Water (HSSW). The HSSW formation is linked to the rejection of salt into the water column as sea ice freezes, especially during winter, in the polynya areas, where the ice is continuously pushed offshore by the strong katabatic winds. The latter one is responsible of the formation of a supercold water mass, the Ice Shelf Water (ISW). The salt supplied by the HSSW recirculated below the Ross Ice Shelf, the latent heat of melting and the heat sink provided by the Ross Ice Shelf give rise to plumes of ISW, characterized by temperatures below the sea-surface freezing point. The dense shelf waters migrate to the continental shelf-break, spill over the shelf edge and descend the continental slope as a shelf-break gravity current, subject to friction and possibly enhanced by topographic channelling. Friction, in particular, breaks the constraint of potential vorticity conservation, counteracting the geostrophic tendency for along slope flow. The density-driven downslope motion or cascading entrains ambient water, namely the lower layer of the CDW, reaches a depth where density is the same and spreads off-slope. In fact, the cascading event is inhibited by friction without entrainment. The downslope processes are important for the ocean and climate system because they play a

Sir Ronald Ross and the Malarial Parasite

Indian Academy of Sciences (India)

1997-08-20

Aug 20, 1997 ... In 1857, a General in the Indian Army, Sir C C G Ross and his wife Matilda .... generally low-caste Indians who required a fee before drinking the water and ... nary demand being made upon their systems, as by fatigue, chill,.

Transcatheter Pulmonary Valve Replacement for Right Ventricular Outflow Tract Conduit Dysfunction After the Ross Procedure

DEFF Research Database (Denmark)

Gillespie, Matthew J; McElhinney, Doff B; Kreutzer, Jacqueline

2015-01-01

BACKGROUND: Right ventricular outflow tract (RVOT) conduit dysfunction is a limitation of the Ross procedure. Transcatheter pulmonary valve replacement (TPVR) could alter the impact of conduit dysfunction and the risk-benefit balance for the Ross procedure. METHODS: Retrospective review of databa......BACKGROUND: Right ventricular outflow tract (RVOT) conduit dysfunction is a limitation of the Ross procedure. Transcatheter pulmonary valve replacement (TPVR) could alter the impact of conduit dysfunction and the risk-benefit balance for the Ross procedure. METHODS: Retrospective review....... Of these, 56 (84%) received a Melody valve; in 5 of the 11 patients who did not, the implant was aborted due to concern for coronary artery compression, and 1 implanted patient required emergent surgery for left coronary compression. The RVOT gradient decreased from a median 38 mm Hg to 13.5 mm Hg (p

Cell wall O-glycoproteins and N-glycoproteins: biosynthesis and some functional aspects.

Directory of Open Access Journals (Sweden)

Eric eNguema-Ona

2014-10-01

Full Text Available Cell wall O-glycoproteins and N-glycoproteins are two types of glycomolecules whose glycans are structurally complex. They are both assembled and modified within the endomembrane system, i.e., the endoplasmic reticulum (ER and the Golgi apparatus, before their transport to their final locations within or outside the cell. In contrast to extensin, the O-glycan chains of arabinogalactan proteins are highly heterogeneous consisting mostly of (i a short oligo-arabinoside chain of three to four residues, and (ii a larger -1,3-linked galactan backbone with -1,6-linked side chains containing galactose, arabinose and, often, fucose, rhamnose or glucuronic acid. The fine structure of arabinogalactan chains varies between, and within plant species, and is important for the functional activities of the glycoproteins. With regards to N-glycans, ER-synthesizing events are highly conserved in all eukaryotes studied so far since they are essential for efficient protein folding. In contrast, evolutionary adaptation of N-glycan processing in the Golgi apparatus has given rise to a variety of organism-specific complex structures. Therefore, plant complex-type N-glycans contain specific glyco-epitopes such as core 1,2-xylose, core 1,3-fucose residues and Lewisa substitutions on the terminal position of the antenna. Like O-glycans, N-glycans of proteins are essential for their stability and function. Mutants affected in the glycan metabolic pathways have provided valuable information on the role of N-/O-glycoproteins in the control of growth, morphogenesis and adaptation to biotic and abiotic stresses. With regards to O-glycoproteins only extensin and arabinogalactan proteins are considered herein. The biosynthesis of these glycoproteins and functional aspects are presented and discussed in this review.

76 FR 41308 - Strata Energy, Inc., Ross In Situ Recovery Uranium Project, Crook County, WY; Notice of Materials...

Science.gov (United States)

2011-07-13

..., Inc., Ross In Situ Recovery Uranium Project, Crook County, WY; Notice of Materials License Application...-4737, or by e-mail to [email protected] . The Ross In Situ Recovery Uranium Project License... source and byproduct materials license at its Ross In Situ Recovery Uranium Project site located in Crook...

Mortimer Hills pegmatite uranium prospect: a Rossing-type uranium deposit in the Gascoyne Province

International Nuclear Information System (INIS)

Carter, J.D.

1984-01-01

A uraninite-bearing pegmatite of large dimensions in the Gascoyne Province is described. The pegmatite is compared with the Rossing uranium ore body of South West Africa and the two are shown to have common characteristics. Exploration recommendations for Rossing-type uranium mineralization in the Gascoyne Province are made

Uranium extraction at Rossing

International Nuclear Information System (INIS)

Kesler, S.B.; Fahrbach, D.O.E.

1982-01-01

Rossing Uranium Ltd. operates a large open pit uranium mine and extraction plant at a remote site in the Namib desert. Production started at the plant in 1978. A ferric leach process was introduced later, and the new leach plant began commissioning in October 1981. The process has proved to be reliable and easily controlled. Ferric iron is supplied through recovery from the acid plant calcine, and levels can be maintained above the design levels. Leach extractions were increased more than expected when this process was adopted, and the throughput has been considerably reduced, allowing cost savings in mining and milling

Note On The Ross Sea Shelf Water Downflow Processes (antarctica)

Science.gov (United States)

Bergamasco, A.; Defendi, V.; Spezie, G.; Budillon, G.; Carniel, S.

In the framework of the CLIMA Project of the Italian National Program for Research in Antarctica, three different experimental data sets were acquired along the continental shelf break; two of them (in 1997 and 2001) close to Cape Adare, the 1998 one in the middle of the Ross Sea (i.e. 75 S, 177 W). The investigations were chosen in order to explore the downslope flow of the bottom waters produced in the Ross Sea, namely the High Salinity Shelf Water (HSSW, the densest water mass of the southern ocean coming from its formation site in the polynya region in Terra Nova bay), and the Ice Shelf Water (ISW, originated below the Ross Ice Shelf and outflowing northward). Both bottom waters spill over the shelf edge and mix with the Circumpolar Deep Water (CDW) contributing to the formation of the Antarctic Bottom Waters (AABW). Interpreting temperature, salinity and density maps in terms of cascading processes, both HSSW and ISW overflows are evidenced during, respectively, 1997 and 1998. During the 2001 acquisition there is no presence of HSSW along the shelf break, nevertheless distribution captures the evidence of a downslope flow process.

CLOUDS IN THE COLDEST BROWN DWARFS: FIRE SPECTROSCOPY OF ROSS 458C

International Nuclear Information System (INIS)

Burgasser, Adam J.; Simcoe, Robert A.; Bochanski, John J.; Saumon, Didier; Mamajek, Eric E.; McMurtry, Craig; Pipher, Judith L.; Forrest, William J.; Cushing, Michael C.; Marley, Mark S.

2010-01-01

Condensate clouds are a salient feature of L dwarf atmospheres, but have been assumed to play little role in shaping the spectra of the coldest T-type brown dwarfs. Here we report evidence of condensate opacity in the near-infrared spectrum of the brown dwarf candidate Ross 458C, obtained with the Folded-Port Infrared Echellette (FIRE) spectrograph at the Magellan Telescopes. These data verify the low-temperature nature of this source, indicating a T8 spectral classification, log 10 L bol /L sun = -5.62 ± 0.03, T eff = 650 ± 25 K, and a mass at or below the deuterium burning limit. The data also reveal enhanced emission at the K band associated with youth (low surface gravity) and supersolar metallicity, reflecting the properties of the Ross 458 system (age = 150-800 Myr, [Fe/H] = +0.2 to +0.3). We present fits of FIRE data for Ross 458C, the T9 dwarf ULAS J133553.45+113005.2, and the blue T7.5 dwarf SDSS J141624.08+134826.7B, to cloudless and cloudy spectral models from Saumon and Marley. For Ross 458C, we confirm a low surface gravity and supersolar metallicity, while the temperature differs depending on the presence (635 +25 -35 K) or absence (760 +70 -45 K) of cloud extinction. ULAS J1335+1130 and SDSS J1416+1348B have similar temperatures (595 +25 -45 K), but distinct surface gravities (log g = 4.0-4.5 cgs versus 5.0-5.5 cgs) and metallicities ([M/H] ∼ +0.2 versus -0.2). In all three cases, cloudy models provide better fits to the spectral data, significantly so for Ross 458C. These results indicate that clouds are an important opacity source in the spectra of young cold T dwarfs and should be considered when characterizing planetary-mass objects in young clusters and directly imaged exoplanets. The characteristics of Ross 458C suggest that it could itself be regarded as a planet, albeit one whose cosmogony does not conform with current planet formation theories.

Ross Ice Drainage System (RIDS) Glaciochemical Analysis, Version 1

Data.gov (United States)

National Aeronautics and Space Administration — The Ross Ice Drainage System (RIDS) project provides a high-resolution record of atmospheric chemical deposition taken from several ice cores and snow pits located...

The Ross Operation in Children and Young Adults: 12-Year Results and Trends From the UK National Database.

Science.gov (United States)

Zebele, Carlo; Chivasso, Pierpaolo; Sedmakov, Christo; Angelini, Gianni; Caputo, Massimo; Parry, Andrew; Stoica, Serban

2014-07-01

To determine UK national trends and results of the Ross operation in relation to all aortic valve interventions. Examination of the UK Congenital Central Cardiac Audit Database for all aortic valve procedures performed between 2000 and 2011 in children (0-16 years) and young adults (16-30 years). A total of 2,206 aortic valve procedures were performed in children and 1,824 in young adults, the proportions in the two groups being: Ross operation (19% vs 15%, respectively), surgical valvoplasty (9.5% vs 4%), surgical valvotomy (9.5% vs 1%), aortic valve replacement (AVR; 11% vs 55%), aortic root replacement (4% vs 18%), and balloon valvoplasty (47% vs 7%). The 30-day and 1-year survival after Ross is 99.3% and 98.7%, respectively, in the last four years achieving 100%. In children, the proportion of balloon valvoplasty increased from an average of 43% in 2000 to 2006 to 53% in 2007 to 2011, whereas the Ross operation decreased from 22% to 16% (P Ross (P Ross operations performed. The year-on-year changes show a significant decreasing trend locally and nationally. Despite an excellent track record, the Ross operation is performed less frequently in the United Kingdom. This report is a first step in comparing treatment modalities at national level. © The Author(s) 2014.

First stage of INTRAMAP: INtegrated Transantarctic Mountains and Ross Sea Area Magnetic Anomaly Project

Directory of Open Access Journals (Sweden)

D. Damaske

1999-06-01

Full Text Available INTRAMAP (INtegrated Transantarctic Mountains and Ross Sea Area Magnetic Anomaly Project is an international effort to merge the magnetic data acquired throughout the "Ross Sea Antarctic Sector" (south of 60°S between 135°-255°E including the Transantarctic Mountains (TAM, the Ross Sea, Marie Byrd Land, and the Pacific coast, and also to begin the compilation efforts for new data over the Wilkes Basin. This project is a component of the continental scale Antarctic Digital Magnetic Anomaly Project (ADMAP. The first stage of INTRAMAP addresses the analysis and merging of GITARA (1991-1994 and GANOVEX (1984 aeromagnetic surveys together with ground magnetic data (1984-1989. The combined data sets cover an area of approximately 30 km2 over Victoria Land and adjacent Ross Sea. Map and profile gridding were implemented to integrate the data sets. These approaches are studied for improving existing strategies to adopt for the whole magnetic compilation effort. The final microlevelled grid that we produce is a new tool for regional interpretation of the main tectonic and geologic features of this sector of Antarctica.

Dimers of beta 2-glycoprotein I mimic the in vitro effects of beta 2-glycoprotein I-anti-beta 2-glycoprotein I antibody complexes

NARCIS (Netherlands)

Lutters, B. C.; Meijers, J. C.; Derksen, R. H.; Arnout, J.; de Groot, P. G.

2001-01-01

Anti-beta(2)-glycoprotein I antibodies are thought to cause lupus anticoagulant activity by forming bivalent complexes with beta(2)-glycoprotein I (beta(2)GPI). To test this hypothesis, chimeric fusion proteins were constructed of the dimerization domain (apple 4) of factor XI and beta(2)GPI. Both a

Giving to Excellence: Generating Philanthropic Support for UK Higher Education. Ross-CASE Report 2016

Science.gov (United States)

Jain, Yashraj

2016-01-01

This report presents findings from the 2016 Ross-CASE Survey of Philanthropic Giving to Universities in UK. The project was conducted by CASE Europe and funded by HEFCE and the Ross-Group. This year's survey comes at a time of great change for the UK charity sector. The historical trend data of previous surveys will be invaluable in helping…

Glycoprotein biosynthesis by human normal platelets

International Nuclear Information System (INIS)

Rodriguez, P.; Bello, O.; Apitz-Castro, R.

1987-01-01

Incorporation of radioactive Man, Gal, Fuc, Glc-N, and NANA into washed human normal platelets and endogenous glycoproteins has been found. Both parameters were time dependent. Analysis of hydrolyzed labeled glycoproteins by paper chromatography revealed that the radioactive monosaccharide incubated with the platelets had not been converted into other sugars. Acid hydrolysis demonstrates the presence of a glycosidic linkage. All the effort directed to the demonstration of the existence of a lipid-sugar intermediate in intact human platelets yielded negative results for Man and Glc-N used as precursors. The incorporation of these sugars into glycoproteins is insensitive to bacitracin, suggesting no involvement of lipid-linked saccharides in the synthesis of glycoproteins in human blood platelets. The absence of inhibition of the glycosylation process in the presence of cycloheximide suggests that the sugars are added to proteins present in the intact platelets. These results support the contention that glycoprotein biosynthesis in human blood platelets observed under our experimental conditions is effected through direct sugar nucleotide glycosylation

STS-74 Mission Specialists McArther and Ross in OPF

Science.gov (United States)

1995-01-01

In Orbiter Processing Facility Bay 2, STS-74 Mission Specialist William 'Bill' McArthur Jr. (left) and Jerry L. Ross are reviewing the configuration of payload elements in the orbiter Atlantis' payload bay. Ross and McArthur are participating in the Crew Equipment Interface Test (CEIT), an opportunity for flight crew members to become familiar with the payload hardware they will be working with on-orbit. Located in Atlantis' payload bay are the Orbiter Docking System and the Docking Module, two pieces of flight hardware that will play a crucial role in the second docking of the Space Shuttle to the Russian Space Station Mir. STS-74 is currently targeted for an early November launch

Critiquing Research Methodology: Comments on Broader Concerns about Complex Statistical Studies. A Response to Ross

Science.gov (United States)

Stapleton, Paul

2006-01-01

Steven Ross's (2005) recent paper empirically measuring the efficacy of formative assessment compared to the summative variety found that the former produced positive effects on some aspects of language learning. In this critique, it is contended that Ross's study is flawed because his two groups of learners, one receiving summative assessment and…

R. E. van der Ross (1921–2017)

African Journals Online (AJOL)

19 Mrt. 2018 ... Sedert sy jeug het Richard Ernest van der Ross. (1921–2017) 'n belangrike rol in die breë Kaapse ge- meenskap gespeel. Hy het hom onderskei as 'n anti- apartheidsaktivis, 'n opvoedkundige, 'n geskied- skrywer en openbare intellektueel, 'n vakbondmens,. 'n gemeenskapsbouer, 'n maatskappydirekteur ...

Ross Ice Shelf, Antarctic Ice and Clouds

Science.gov (United States)

1991-01-01

In this view of Antarctic ice and clouds, (56.5S, 152.0W), the Ross Ice Shelf of Antarctica is almost totally clear, showing stress cracks in the ice surface caused by wind and tidal drift. Clouds on the eastern edge of the picture are associated with an Antarctic cyclone. Winds stirred up these storms have been known to reach hurricane force.

Experiencia a largo plazo con la operación de Ross en pacientes pediátricos

Directory of Open Access Journals (Sweden)

José M. Caffarena Calvar

2008-07-01

Conclusions: The Ross and Ross-Konno operations have changed the prognosis of pediatric patients with complex disease of the valve and aortic root. There is growth of the neo-aortic root in young patients. Sinotubular dilatation of the autograft with neoaortic valve insufficiency has been detected in patients with previously dilated aortic root when root replacement technique was performed. The best results are obtained in the younger andlower weight patients with complex left ventricular outflow obstruction without root dilatation. A close follow-up of patients with aortic root dilatation is mandatory to definitely validate the Ross procedure over aortic prosthesis.

Why can rossing uranium mine keep mining even in low price conditions of uranium market

International Nuclear Information System (INIS)

Tan Chenglong

2004-01-01

Rossing uranium mine is the only operating uranium mine in the world where the uranium occurs in intrusive alaskite. In the past 10 years, uranium market regressed in the world, uranium production weakened, expenditures of capital for uranium exploration were insufficient. Uranium spot market price rapidly decreased from $111.8/kg U in late 1970's to $22.1/kg U in mid-1990's. Why can Rossing uranium mine mined with traditional underground and open pit operation can keep running even in low price conditions of uranium market? Augumenting research on the deposit, mineral and technology, decreasing production cost and improving selling strategy can not only maintain Rossing's uranium production at present, but also ensure sustainable development in the coming 15 years. Exploration of low-costed uranium deposits is very important. However, obvious economic benefits can be obtained, as Rossing uranium mine does, by augumenting geological-economical research on the known uranium deposits of hard-rock type and by using new techniques to improve the conventional techniques in the uranium mine development. (authors)

Special design issues related to the G. Ross Lord Dam constructed in Metropolitan Toronto

Energy Technology Data Exchange (ETDEWEB)

Sowa, V.A. [Jacques Whitford and Associates Ltd., Vancouver, BC (Canada); Tawil, A.H. [Acres International Ltd., Niagara Falls, ON (Canada); Haley, D.R. [Toronto Region and Conservation Authority, Downsview, ON (Canada)

2002-07-01

This paper describes the special considerations required to build a flood control dam in a metropolitan area that holds major city infrastructures such as power transmission towers, pipelines, sanitary sewers and graveyards. The paper refers to the G. Ross Lord Dam, a 20 m high earth fill flood control dam which was constructed in 1973 on the West Branch of the Don River in Toronto. It was built following recommendations after Hurricane Hazel caused widespread flooding and the death of 81 people in 1954. The dam includes a concrete chute spillway and stilling basin. The geotechnical design of the dam was described along with the dam structures and the methods used to flood proof the infrastructure. The dam has a sloping impervious core and an upstream blanket to reduce seepage. Seepage control is provided by a drainage blanket and a chimney drain. A main overflow spillway was constructed on the south abutment, and a low level outlet was constructed at the base of the dam to accommodate normal river flows through the dam. Most of the water level control during a flood event is provided by the main overflow spillway. Spillway slab anchor keys prevent down slope creep of the slabs. The dam, the spillway and the reservoir structure have performed well since construction. 6 refs., 10 figs.

Valve-sparing root replacement for freestanding pulmonary autograft aneurysm after the Ross procedure.

Science.gov (United States)

Ratschiller, Thomas; Eva, Sames-Dolzer; Schimetta, Wolfgang; Paulus, Patrick; Müller, Hannes; Zierer, Andreas; Mair, Rudolf

2018-02-20

Autograft dilatation is the main long-term complication following the Ross procedure using the freestanding root replacement technique. We reviewed our 25-year experience with the Ross procedure with a special emphasis on valve-sparing reoperations. From 1991 to 2016, 153 patients (29.6 ± 16.6 years; 29.4% pediatric) underwent a Ross operation at our institution with implantation of the autograft as freestanding root replacement. The follow-up is 98.7% complete with a mean of 12.2 ± 5.5 years. Mortality at 30-days was 2.0%. Echocardiography documented no or trivial aortic regurgitation in 99.3% of the patients at discharge. Survival probability at 20 years was 85.4%. No case of autograft endocarditis occurred. Autograft deterioration rate was 2.01% per patient-year, and freedom from autograft reoperation was 75.3% at 15 years. A reoperation for autograft aneurysm was required in 35 patients (22.9%) at a mean interval of 11.1 ± 4.6 years after the Ross procedure. A valve-sparing root replacement was performed in 77% of patients, including 10 David and 17 Yacoub procedures with no early mortality. Three patients required prosthetic valve replacement within 2 years after a Yacoub operation. At latest follow-up, 92% of all surviving patients still carry the pulmonary autograft valve. Freedom from autograft valve replacement was 92.1% at 15 years. Using the David or Yacoub techniques, the autograft valve can be preserved in the majority of patients with root aneurysms after the Ross procedure. Reoperations can be performed with no early mortality, a good functional midterm result, and an acceptable reintervention rate. Copyright © 2018 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.

Late Quaternary climatic changes in the Ross Sea area, Antarctica

International Nuclear Information System (INIS)

Brambati, A.; Melis, R.; Quaia, T.; Salvi, G.

2002-01-01

Ten cores from the Ross Sea continental margin were investigated to detect Late Quaternary climatic changes. Two main climatic cycles over the last 300,000 yr (isotope stages 1-8) were recognised in cores from the continental slope, whereas minor fluctuations over the last 30,000 yr were found in cores from the continental shelf. The occurrence of calcareous taxa within the Last Glacial interval and their subsequent disappearance reveal a general raising of the CCD during the last climatic cycle. In addition, periodical trends of c. 400, c. 700, and c. 1400 yr determined on calcareous foraminifers from sediments of the Joides Basin, indicate fluctuations of the Ross Ice Shelf between 15 and 30 ka BP. (author). 24 refs., 5 figs

Ross Ice Shelf and the Queen Maude Mounains, Antarctica

Science.gov (United States)

1991-01-01

Part of the Ross Ice Shelf and the Queen Maude Mounains of Antarctica (55.5N, 178.0W) are in the background of this scene, oriented toward the south. Low stratocumulus clouds are predominant throughout most of the scene.

Is the Ross procedure a riskier operation? Perioperative outcome comparison with mechanical aortic valve replacement in a propensity-matched cohort.

Science.gov (United States)

Bouhout, Ismail; Noly, Pierre-Emmanuel; Ghoneim, Aly; Stevens, Louis-Mathieu; Cartier, Raymond; Poirier, Nancy; Bouchard, Denis; Demers, Philippe; El-Hamamsy, Ismail

2017-01-01

The aim of this study was to compare perioperative outcomes in young adults following isolated Ross procedure versus mechanical aortic valve replacement (AVR) in a high-volume centre. From 2007 to 2015, 337 elective isolated mechanical AVRs and 137 Ross procedures were performed in young adults (Ross cohort versus six (9%) in the mechanical AVR cohort (P = 0.49). A significant increase in serum creatinine (>2-fold increase) was more commonly observed after the Ross procedure (11 vs 1%; P = 0.03), but there was no significant difference in the rate of temporary dialysis. Twenty-seven patients (39%) required ≥1 blood product transfusion in the Ross group, whereas 21 patients (31%) did so in the mechanical AVR group (P = 0.47). Median hospital length of stay was similar in both the groups (6 days). There are no differences in mortality or major perioperative outcomes in adults undergoing an isolated Ross procedure or mechanical AVR. © The Author 2016. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

ROSS Skills, Knowledge, and Abilities Training Evaluation. Gaps and Recommendations

Energy Technology Data Exchange (ETDEWEB)

Ala, Maureen [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Gruidl, Jeremiah [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Buddemeier, Brooke [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2015-09-30

This document describes the development of the ROSS SKAs, the cross-mapping of the SKAs to the available training, identifies gaps in the SKA and training, and provides recommendations to address those gaps.

TEST OF THE CHEN-ROLL-ROSS MACROECONOMIC FACTOR MODEL: EVIDENCE FROM CROATIAN STOCK MARKET

Directory of Open Access Journals (Sweden)

Denis Dolinar

2015-12-01

Full Text Available This paper empirically examines the well-known Chen-Roll-Ross model on the Croatian stock market. Modifications of definitions of the Chen-Roll-Ross model variables showed as necessary because of doubtful availability and quality of input data needed. Namely, some macroeconomic and market variables are not available in the originally defined form or do not exist. In that sense this paper gives some alternative definitions for some model variables. Also, in order to improve statistical analysis, in this paper we have modified Fama-MacBeth technique in the way that second-pass regression was substituted with panel regression analysis. Based on the two-pass regression analysis of returns of 34 Croatian stocks on 4 macroeconomic variables during the seven-and-half-year observation period the following conclusion is made. In contrast to the results of Chen, Roll and Ross (1986 for the U.S. stock market, their model is not successful when describing a risk-return relation of Croatian stocks. Nevertheless, one observed version of the Chen-RollRoss model showed certain statistical significance. Namely, two risk factors in that version of the model were statistically significant: default premium, measured as risk premium for the corporate short-term bank loan financing, and term structure premium, measured on short-run basis.

Ammonia transport in the kidney by Rhesus glycoproteins

Science.gov (United States)

Verlander, Jill W.

2014-01-01

Renal ammonia metabolism is a fundamental element of acid-base homeostasis, comprising a major component of both basal and physiologically altered renal net acid excretion. Over the past several years, a fundamental change in our understanding of the mechanisms of renal epithelial cell ammonia transport has occurred, replacing the previous model which was based upon diffusion equilibrium for NH3 and trapping of NH4+ with a new model in which specific and regulated transport of both NH3 and NH4+ across renal epithelial cell membranes via specific membrane proteins is required for normal ammonia metabolism. A major advance has been the recognition that members of a recently recognized transporter family, the Rhesus glycoprotein family, mediate critical roles in renal and extrarenal ammonia transport. The erythroid-specific Rhesus glycoprotein, Rh A Glycoprotein (Rhag), was the first Rhesus glycoprotein recognized as an ammonia-specific transporter. Subsequently, the nonerythroid Rh glycoproteins, Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg), were cloned and identified as ammonia transporters. They are expressed in specific cell populations and membrane domains in distal renal epithelial cells, where they facilitate ammonia secretion. In this review, we discuss the distribution of Rhbg and Rhcg in the kidney, the regulation of their expression and activity in physiological disturbances, the effects of genetic deletion on renal ammonia metabolism, and the molecular mechanisms of Rh glycoprotein-mediated ammonia transport. PMID:24647713

Trends in the breeding population and driving factors of Adélie penguin in the Ross Sea

Science.gov (United States)

He, H.; Li, X.; Cheng, X.

2017-12-01

Ross Sea regions have been characterized by high penguin-chick-rearing habitat suitability in the recent past. Many studies have been done to study the Adélie penguins in the Ross Sea. However, the data they used both had advantages and drawbacks. Besides, little quantitative analysis were carried out to study the impact factors on the penguin population change. In this study, penguin population data from MAPPPD (Mapping application for penguin populations and projected dynamics) and IBA (Important bird areas in Antarctica) were integrated and analyzed to study the distribution and trends in the breeding population of Adélie penguin over time in the Ross Sea. In addition, linear fitting method for spatial data in time series were used to study the driving factors such as 2m-temperature, sea ice cover and chlorophyll-a concentration which can quantify phytoplankton blooms. Results indicated that there were 45 Adélie penguin colonies in the Ross Sea. Cape Adare and Cape Crozier were two biggest colonies on which current Adélie penguin abundance were 428516 and 280787 breeding pairs, respectively. Among these colonies, penguin population on 28 colonies increased, on 5 colonies decreased and on 5 colonies remained no change over time, and there were also 5 new colonies and one colony which were extinct. It was found that Adélie penguin population in most of colonies in the Ross Sea increased, which meant that Adélie penguins in the Ross Sea were "climate change winners". The main reasons for the increase in Adélie penguin population in the Ross Sea might be the rise in 2m-temperature and the increase in sea ice cover and phytoplankton. Higher temperatures have resulted in glacial retreat and snow melting, which leads to an increase in available habitat for penguins. The increased sea ice and phytoplankton might positively affect the abundance of Antarctic krill that was the major prey item for Adélie penguins in Antarctic.

DVD Review: "The Silver Fez" Directed by Lloyd Ross (2009 ...

African Journals Online (AJOL)

Abstract. Producer: Lloyd Ross, Joelle Chesselet. Sound design: Warrick Sony. Cast: Abubakar Davids, Mogamat Zain Benjamin. Approx. 87 min. Distributor: IRIS. ZAR 129.99. Journal of the Musical Arts in Africa, Volume 9 2012, 89–91 ...

Solubilization of glycoproteins of envelope viruses by detergents

International Nuclear Information System (INIS)

Berezin, V.E.; Zaides, V.M.; Artamsnov, A.F.; Isaeva, E.S.; Zhdanov, V.M.

1986-01-01

The action of a number of known ionic and nonionic detergents, as well as the new nonionic detergent MESK, on envelope viruses was investigated. It was shown that the nonionic detergents MESK, Triton X-100, and octyl-β-D-glucopyranoside selectively solubilize the outer glycoproteins of the virus particles. The nonionic detergent MESK has the mildest action. Using MESK, purified glycoproteins of influenza, parainfluenza, Venezuelan equine encephalomyelitis, vesicular stomatitis, rabies, and herpes viruses were obtained. The procedure for obtaining glycoproteins includes incubation of the virus suspension with the detergent MESK, removal of subvirus structures by centrifuging, and purification of glycoproteins from detergents by dialysis. Isolated glycoproteins retain a native structure and biological activity and possess high immunogenicity. The detergent MESK is promising for laboratory tests and with respect to the production of subunit vaccines

Survival comparison of the Ross procedure and mechanical valve replacement with optimal self-management anticoagulation therapy: propensity-matched cohort study.

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Mokhles, M Mostafa; Körtke, Heinrich; Stierle, Ulrich; Wagner, Otto; Charitos, Efstratios I; Bogers, Ad J J C; Gummert, Jan; Sievers, Hans-Hinrich; Takkenberg, Johanna J M

2011-01-04

It is suggested that in young adults the Ross procedure results in better late patient survival compared with mechanical prosthesis implantation. We performed a propensity score-matched study that assessed late survival in young adult patients after a Ross procedure versus that after mechanical aortic valve replacement with optimal self-management anticoagulation therapy. We selected 918 Ross patients and 406 mechanical valve patients 18 to 60 years of age without dissection, aneurysm, or mitral valve replacement who survived an elective procedure (1994 to 2008). With the use of propensity score matching, late survival was compared between the 2 groups. Two hundred fifty-three patients with a mechanical valve (mean follow-up, 6.3 years) could be propensity matched to a Ross patient (mean follow-up, 5.1 years). Mean age of the matched cohort was 47.3 years in the Ross procedure group and 48.0 years in the mechanical valve group (P=0.17); the ratio of male to female patients was 3.2 in the Ross procedure group and 2.7 in the mechanical valve group (P=0.46). Linearized all-cause mortality rate was 0.53% per patient-year in the Ross procedure group compared with 0.30% per patient-year in the mechanical valve group (matched hazard ratio, 1.86; 95% confidence interval, 0.58 to 5.91; P=0.32). Late survival was comparable to that of the general German population. In comparable patients, there is no late survival difference in the first postoperative decade between the Ross procedure and mechanical aortic valve implantation with optimal anticoagulation self-management. Survival in these selected young adult patients closely resembles that of the general population, possibly as a result of highly specialized anticoagulation self-management, better timing of surgery, and improved patient selection in recent years.

Evaluation of the Ross fast solution of Richards' equation in unfavourable conditions for standard finite element methods

International Nuclear Information System (INIS)

Crevoisier, D.; Voltz, M.; Chanzy, A.

2009-01-01

Ross [Ross PJ. Modeling soil water and solute transport - fast, simplified numerical solutions. Agron J 2003;95:1352-61] developed a fast, simplified method for solving Richards' equation. This non-iterative 1D approach, using Brooks and Corey [Brooks RH, Corey AT. Hydraulic properties of porous media. Hydrol. papers, Colorado St. Univ., Fort Collins: 1964] hydraulic functions, allows a significant reduction in computing time while maintaining the accuracy of the results. The first aim of this work is to confirm these results in a more extensive set of problems, including those that would lead to serious numerical difficulties for the standard numerical method. The second aim is to validate a generalisation of the Ross method to other mathematical representations of hydraulic functions. The Ross method is compared with the standard finite element model, Hydrus-1D [Simunek J, Sejna M, Van Genuchten MTh. The HYDRUS-1D and HYDRUS-2D codes for estimating unsaturated soil hydraulic and solutes transport parameters. Agron Abstr 357; 1999]. Computing time, accuracy of results and robustness of numerical schemes are monitored in 1D simulations involving different types of homogeneous soils, grids and hydrological conditions. The Ross method associated with modified Van Genuchten hydraulic functions [Vogel T, Cislerova M. On the reliability of unsaturated hydraulic conductivity calculated from the moisture retention curve. Transport Porous Media 1988:3:1-15] proves in every tested scenario to be more robust numerically, and the compromise of computing time/accuracy is seen to be particularly improved on coarse grids. Ross method run from 1.25 to 14 times faster than Hydrus-1D. (authors)

Transcatheter versus surgical valve replacement for a failed pulmonary homograft in the Ross population.

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Alassas, Khadija; Mohty, Dania; Clavel, Marie Annick; Husain, Aysha; Hijji, Talal; Aljoufan, Mansour; Alhalees, Zohair; Fadel, Bahaa M

2018-04-01

Patients who undergo the Ross procedure are at increased risk of pulmonary valve (PV) homograft dysfunction. For those who require reintervention on the homograft, transcatheter PV replacement (tPVR) provides a less invasive therapeutic option than surgical PVR (sPVR). We examined the outcomes following tPVR versus sPVR in a cohort of patients who underwent the Ross procedure. We performed a retrospective analysis of Ross patients age ≥14 years who underwent tPVR (n = 47) or sPVR (n = 41) at our institution. The patients' clinical and echocardiographic data were reviewed. Baseline parameters, including demographic data and left ventricular and right ventricular (RV) systolic function, were similar in the 2 groups. The mean follow-up was 56 ± 24 months for the tPVR group and 89 ± 46 months for the sPVR group (P Ross patients who require reintervention on the PV homograft, both tPVR and sPVR provide low procedural mortality and comparable midterm outcome with no significant difference in mortality or PV reintervention. However, IE is more common following tPVR. A larger randomized study is needed to determine the role of each procedure in patient management. Copyright © 2017 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.

Nucleic acid-binding glycoproteins which solubilize nucleic acids in dilute acid: re-examination of the Ustilago maydis glycoproteins

Energy Technology Data Exchange (ETDEWEB)

Unrau, P.; Champ, D.R.; Young, J.L.; Grant, C.E.

1980-01-01

Holloman reported the isolation from Ustilago maydis of a glycoprotein which prevented the precipitation of nucleic acids in cold 5% trichloroacetic acid. Two glycoprotein fractions from U. maydis with this nucleic acid-solubilizing activity were isolated in our laboratory using improved purification procedures. The activity was not due to nuclease contamination. The glycoproteins are distinguished by: their ability to bind to concanavalin A-Sepharose; their differential binding to double- and single-stranded deoxyribonucleic acid, and to ribonucleic acid; their molecular weights (46,000 and 69,000); and the relative amounts present in growing versus nongrowing cells. Both fractions required sulfhydryl-reducing conditions for optimal yields, specific activity, and stability. Nucleic acid binding was cooperative, the minimum number of glycoproteins required to make a native T7 DNA molecule soluble in dilute acid being estimated at 2 and 15, respectively.

Suramin is a potent inhibitor of Chikungunya and Ebola virus cell entry.

Science.gov (United States)

Henß, Lisa; Beck, Simon; Weidner, Tatjana; Biedenkopf, Nadine; Sliva, Katja; Weber, Christopher; Becker, Stephan; Schnierle, Barbara S

2016-08-31

Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes high fever, rash, and recurrent arthritis in humans. It has efficiently adapted to Aedes albopictus, which also inhabits temperate regions and currently causes large outbreaks in the Caribbean and Latin America. Ebola virus (EBOV) is a member of the filovirus family. It causes the Ebola virus disease (EDV), formerly known as Ebola hemorrhagic fever in humans and has a mortality rate of up to 70 %. The last outbreak in Western Africa was the largest in history and has caused approximately 25,000 cases and 10,000 deaths. For both viral infections no specific treatment or licensed vaccine is currently available. The bis-hexasulfonated naphthylurea, suramin, is used as a treatment for trypanosome-caused African river blindness. As a competitive inhibitor of heparin, suramin has been described to have anti-viral activity. We tested the activity of suramin during CHIKV or Ebola virus infection, using CHIKV and Ebola envelope glycoprotein pseudotyped lentiviral vectors and wild-type CHIKV and Ebola virus. Suramin efficiently inhibited CHIKV and Ebola envelope-mediated gene transfer while vesicular stomatitis virus G protein pseudotyped vectors were only marginally affected. In addition, suramin was able to inhibit wild-type CHIKV and Ebola virus replication in vitro. Inhibition occurred at early time points during CHIKV infection. Suramin, also known as Germanin or Bayer-205, is a market-authorized drug, however shows significant side effects, which probably prevents its use as a CHIKV drug, but due to the high lethality of Ebola virus infections, suramin might be valuable against Ebola infections.

The Satellite Passive-Microwave Record of Sea Ice in the Ross Sea Since Late 1978

Science.gov (United States)

Parkinson, Claire L.

2009-01-01

Satellites have provided us with a remarkable ability to monitor many aspects of the globe day-in and day-out and sea ice is one of numerous variables that by now have quite substantial satellite records. Passive-microwave data have been particularly valuable in sea ice monitoring, with a record that extends back to August 1987 on daily basis (for most of the period), to November 1970 on a less complete basis (again for most of the period), and to December 1972 on a less complete basis. For the period since November 1970, Ross Sea sea ice imagery is available at spatial resolution of approximately 25 km. This allows good depictions of the seasonal advance and retreat of the ice cover each year, along with its marked interannual variability. The Ross Sea ice extent typically reaches a minimum of approximately 0.7 x 10(exp 6) square kilometers in February, rising to a maximum of approximately 4.0 x 10(exp 6) square kilometers in September, with much variability among years for both those numbers. The Ross Sea images show clearly the day-by-day activity greatly from year to year. Animations of the data help to highlight the dynamic nature of the Ross Sea ice cover. The satellite data also allow calculation of trends in the ice cover over the period of the satellite record. Using linear least-squares fits, the Ross Sea ice extent increased at an average rate of 12,600 plus or minus 1,800 square kilometers per year between November 1978 and December 2007, with every month exhibiting increased ice extent and the rates of increase ranging from a low of 7,500 plus or minus 5,000 square kilometers per year for the February ice extents to a high of 20,300 plus or minus 6,100 kilometers per year for the October ice extents. On a yearly average basis, for 1979-2007 the Ross Sea ice extent increased at a rate of 4.8 plus or minus 1.6 % per decade. Placing the Ross Sea in the context of the Southern Ocean as a whole, over the November 1978-December 2007 period the Ross Sea had

Distribution of total alkalinity and pH in the Ross Sea (Antarctica waters during austral summer 2008

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Paola Rivaro

2014-10-01

Full Text Available Measurements of total alkalinity (AT and pH were made in the Ross Sea in January–February 2008 in order to characterize the carbonate system in the Ross Sea and to evaluate the variability associated with different water masses. The main water masses of the Ross Sea, Antarctic Surface Water, High Salinity Shelf Water (HSSW, Deep Ice Shelf Water, Circumpolar Deep Water (CDW and Antarctic Bottom Water, were identified on the basis of the physical and chemical data. In particular, the AT ranged between 2275 and 2374 µmol kg−1 with the lowest values in the surface waters (2275–2346 µmol kg−1, where the influence of the sea-ice melting and of the variability of the physical properties was significant. In the deep layers of the water column, the AT maxima were measured in correspondence to the preferential pathways of the spreading HSSW. The pH had variable values in the surface layer (7.890–8.033 with the highest values in Terra Nova Bay and Ross Sea polynyas. A low pH (7.969±0.025 traced the intrusion of the CDW in the Ross Sea shelf area. All samples revealed waters that were oversaturated with respect to both calcite and aragonite, but near corrosive levels of aragonite saturation state (Ω ca. 1.1–1.2 were associated with the entrainment of CDW over the slope. Aragonite undersaturation is of particular concern for the zooplankton species comprising to calcifying organisms such as pteropods. The partial pressure of CO2 at the sea surface was undersaturated with respect to the atmospheric value, particularly in Terra Nova Bay and the Ross Sea polynyas, but a large variability in the sea–air CO2 fluxes was observed associated with different responses in the strength of the biological and physical processes.

A Q-Methodological Study of the Kubler-Ross Stage Theory.

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Metzger, Anne M.

1979-01-01

Investigated the correspondence between stage changes hypothesized by the Kubler-Ross theory and the perception of the course of illness by seriously ill patients and their spouses. Supported the use of Q-methodology as a research procedure for investigations of terminal illness. (Author)

Märten Ross sõnastas majanduskriisi õppetunnid / Argo Ideon

Index Scriptorium Estoniae

Ideon, Argo, 1966-

2011-01-01

Ajutiselt peaministri majandus- ja rahandusnõunikuna töötanud endine Eesti Panga asepresident Märten Ross kirjutas loetelu õppetundidest, mida Eesti majanduspoliitika peaks arvestama 2008.-2009. aasta kriisi põhjal. Rossi 10 punkti lühidalt. Rahandusminister Jürgen Ligi ning Eesti Panga rahapoliitika ja majandusuuringute osakonna asejuhataja Martin Lindpere arvamus

On the dense water spreading off the Ross Sea shelf (Southern Ocean)

Science.gov (United States)

Budillon, G.; Gremes Cordero, S.; Salusti, E.

2002-07-01

In this study, current meter and hydrological data obtained during the X Italian Expedition in the Ross Sea (CLIMA Project) are analyzed. Our data show a nice agreement with previous data referring to the water masses present in this area and their dynamics. Here, they are used to further analyze the mixing and deepening processes of Deep Ice Shelf Water (DISW) over the northern shelf break of the Ross Sea. In more detail, our work is focused on the elementary mechanisms that are the most efficient in removing dense water from the shelf: either classical mixing effects or density currents that interact with some topographic irregularity in order to drop to deeper levels, or also the variability of the Antarctic Circumpolar Current (ACC) which, in its meandering, can push the dense water off the shelf, thus interrupting its geostrophic flow. We also discuss in detail the (partial) evidence of dramatic interactions of the dense water with bottom particulate, of geological or biological origin, thus generating impulsive or quasi-steady density-turbidity currents. This complex interaction allows one to consider bottom particular and dense water as a unique self-interacting system. In synthesis, this is a first tentative analysis of the effect of bottom particulate on the dense water dynamics in the Ross Sea.

Persistent organic pollutants in soils and sediments from James Ross Island, Antarctica

International Nuclear Information System (INIS)

Klanova, Jana; Matykiewiczova, Nina; Macka, Zdenek; Prosek, Pavel; Laska, Kamil; Klan, Petr

2008-01-01

Soil and sediment samples from James Ross Island were analyzed for their PCB, OCP and PAH contents. Soil concentrations ranged between 0.51 and 1.82 ng g -1 for seven indicator PCB congeners, between 0.49 and 1.34 ng g -1 for HCH congeners, between 0.51 and 3.68 ng g -1 for the sum of p,p'-DDT, DDE, and DDD, and between 34.9 and 171 ng g -1 for the sum of 16 EPA PAHs. Sediment levels from 0.32 to 0.83 ng g -1 were found for PCBs, from 0.14 to 0.76 ng g -1 for HCHs, from 0.19 to 1.15 ng g -1 for DDTs, and from 1.4 to 205 ng g -1 for PAHs. A prevalence of low-mass PAHs, less chlorinated PCBs, and more volatile chemicals indicates that the long-range atmospheric transport from populated areas of Africa, South America, and Australia is the most probable contamination source for the solid matrices in James Ross Island. - A survey of soil, sediment and air contamination in James Ross Island, Antarctica serves as a baseline study for evaluation of an anthropogenic impact of the new research facility

An improved radioimmunoassay for urinary Tamm-Horsfall glycoprotein

International Nuclear Information System (INIS)

Dawnay, A.B. St. J.; Thornley, C.; Cattell, W.R.

1982-01-01

A rapid specific radioimmunoassay has been used to measure Tamm-Horsfall glycoprotein (TH glycoprotein) in urine, and the method described. The apparent concentration increased with increasing dilution of urine in water, reaching a plateau at 1 in 20. This increase was greater the higher the osmolality and TH glycoprotein concentration and the lower the pH of the original sample. The apparent concentration of TH glycoprotein in neat or diluted urine was not affected by freezing or by storage at 4 0 C or room temperature for at least 2 days. A physiological range for the urinary excretion rate was established as 22-56 mg/24h, (considerably higher than the amount present in serum) based on samples from 29 individuals with normal renal function, as defined by their creatinine clearance. There was no significant correlation between serum concentrations of TH glycoprotein and its urinary excretion rate, nor between urinary excretion rate and creatinine clearance. (author)

Orthobunyavirus ultrastructure and the curious tripodal glycoprotein spike.

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Thomas A Bowden

Full Text Available The genus Orthobunyavirus within the family Bunyaviridae constitutes an expanding group of emerging viruses, which threaten human and animal health. Despite the medical importance, little is known about orthobunyavirus structure, a prerequisite for understanding virus assembly and entry. Here, using electron cryo-tomography, we report the ultrastructure of Bunyamwera virus, the prototypic member of this genus. Whilst Bunyamwera virions are pleomorphic in shape, they display a locally ordered lattice of glycoprotein spikes. Each spike protrudes 18 nm from the viral membrane and becomes disordered upon introduction to an acidic environment. Using sub-tomogram averaging, we derived a three-dimensional model of the trimeric pre-fusion glycoprotein spike to 3-nm resolution. The glycoprotein spike consists mainly of the putative class-II fusion glycoprotein and exhibits a unique tripod-like arrangement. Protein-protein contacts between neighbouring spikes occur at membrane-proximal regions and intra-spike contacts at membrane-distal regions. This trimeric assembly deviates from previously observed fusion glycoprotein arrangements, suggesting a greater than anticipated repertoire of viral fusion glycoprotein oligomerization. Our study provides evidence of a pH-dependent conformational change that occurs during orthobunyaviral entry into host cells and a blueprint for the structure of this group of emerging pathogens.

Setting the scientistic cat among the humanist pigeons Don Ross ...

African Journals Online (AJOL)

implications of cognitive science and economics for each other. Ross makes neoclassical economics central to the unification of the behavioural sciences, and defends its fundamental health against its critics. He locates the source of the empirical and conceptual problems besetting neoclassical economics in the mistaken ...

The glycoprotein of measles virus

International Nuclear Information System (INIS)

Anttonen, O.; Jokinen, M.; Salmi, A.; Vainionpaeae, R.; Gahmberg, C.G.

1980-01-01

Measles virus was propagated in VERO cells and purified from the culture supernatants by two successive tartrate-density-gradient centrifugations. Surface carbohydrates were labelled both in vitro and in vivo with 3 H after treatment with galactose oxidase/NaB 3 H 4 or with [ 3 H]glucosamine. The major labelled glycoprotein in measles virions had a mol.wt. of 79000. After labelling with periodate/NaB 3 H 4 , which would result in specific labelling of sialic acid residues, the 79000-mol.wt. glycoprotein was very weakly labelled. This suggested that there is no or a very low amount of sialic acid in the virions. Further analysis of the glycoprotein showed that galactose is the terminal carbohydrate unit in the oligosaccharide, and the molecular weight of the glycopeptide obtained after Pronase digestion is about 3000. The oligosaccharide is attached to the polypeptide through an alkali-stable bond, indicating a N-glycosidic asparagine linkage. (author)

Outcomes and Short-Term Follow-Up in Complex Ross Operations in Pediatric Patients Undergoing Damus-Kaye-Stansel Takedown.

Science.gov (United States)

Baird, Christopher W; Zurakowski, David; Bueno, Alejandra; Borisuk, Michele J; Raju, Vijayakumar; Mokashi, Suyog A; Emani, Sitaram; Marx, Gerald R; Del Nido, Pedro J

2016-01-01

Review echocardiography and outcomes before and after Ross procedures, including patients undergoing biventricular conversion with Damus-Kaye-Stansel (DKS) takedown. A retrospective review was performed on 62 patients undergoing simple (control group) and complex Ross procedures, including 12 patients who underwent biventricular conversion with Ross operation and DKS takedown (complex). Echocardiography was reviewed preoperatively and at discharge and late follow-up. Kaplan-Meier estimates of patient survival and freedom from reintervention were obtained. In all, 62 patients had a median age of 4.5 years (interquartile range [IQR]: 1-12.5), weight of 16.4kg (IQR: 8-41), and follow-up of 3.8 years (IQR: 1.3-6). The complex DKS takedown group had 2 deaths, no neoaortic valve or root reinterventions, and 3 right ventricular outflow tract (VOT) reinterventions. There were no differences from the control group in left VOT or right VOT reinterventions. Neither group showed differences between pre- and late follow-up aortic root and ascending aorta dimensions, and no correlations were found among preoperative pulmonary valve (PV) size, late aortic regurgitation (AR), aortic root, or ascending aortic Z-scores. Aortic valve size increased from discharge to late follow-up for both groups (P ≤ .05); 90% of patients at late follow-up had mild or less AR with similar distributions in severity between complex and control groups. Severity of late AR showed no correlation with preoperative PV size and is independent of it. The Ross procedure has good short-term results in simple and complex patients and should be considered in those undergoing Ross operation with biventricular conversion and DKS takedown. Moreover, native PV size should not be a contraindication for Ross procedure. Copyright © 2016 Elsevier Inc. All rights reserved.

Astrometric studies of the regions BD + 17 degree 4946, BD -5 degree 3071, and Ross 730-1

International Nuclear Information System (INIS)

Russell, J.; Gatewood, G.

1975-01-01

Positions, proper motions, and absolute parallaxes were found for 46 stars in the regions of BD +17 degree4946, BD -5 degree3071, and Ross 730-1. BD +17 degree4946 showed some evidence of irregular variations in position and magnitude. The residuals for Ross 730-1 were used in a preliminary study to test for an appropriate weighting system; a weak weighting system was indicated. (auth)

Replacement of the murine leukemia virus (MLV) envelope gene with a truncated HIV envelope gene in MLV generates a virus with impaired replication capacity

International Nuclear Information System (INIS)

Nack, Ursula; Schnierle, Barbara S.

2003-01-01

Murine leukemia virus (MLV) capsid particles can be efficiently pseudotyped with a variant of the HIV-1 envelope protein (Env) containing the surface glycoprotein gp120-SU and a carboxyl-terminally truncated transmembrane (TM) protein, with only seven cytoplasmic amino acids. MLV/HIV pseudotyped vector particles acquire the natural host tropism of HIV-1 and their entry is dependent on the presence of CD4 and an appropriate co-receptor on the surface of the target cell. We describe here the construction of chimeric MLV/HIV proviruses containing the truncated HIV envelope gene. The MLV/HIV provirus was generated by direct replacement of the MLV envelope gene with HIV Env coding sequences either with or without the additional inclusion of the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE). Chimeric MLV/HIV particles could be generated from transfected 293T cells and were able to infect CD4/CXCR4-positive target cells. However, the second round of infection of target cells was severely impaired, despite the fact that the WPRE element enhanced the amount of viral mRNA detected. Viral particles released from infected cells showed reduced HIV Env incorporation, indicating that additional factors required for efficient replication of MLV/HIV pseudotyped viruses are missing

South Polar Skua breeding populations in the Ross Sea assessed from demonstrated relationship with Adélie Penguin numbers

Science.gov (United States)

Wilson, Deborah J.; Lyver, Phil O'B.; Greene, Terry C.; Whitehead, Amy L.; Dugger, Catherine; Karl, Brian J.; Barringer, James R. F.; McGarry, Roger; Pollard, Annie M.; Ainley, David G.

2017-01-01

In the Ross Sea region, most South Polar Skuas (Stercorarius maccormicki) nest near Adélie Penguin (Pygoscelis adeliae) colonies, preying and scavenging on fish, penguins, and other carrion. To derive a relationship to predict skua numbers from better-quantified penguin numbers, we used distance sampling to estimate breeding skua numbers within 1000 m of 5 penguin nesting locations (Cape Crozier, Cape Royds, and 3 Cape Bird locations) on Ross Island in 3 consecutive years. Estimated numbers of skua breeding pairs were highest at Cape Crozier (270,000 penguin pairs; 1099 and 1347 skua pairs in 2 respective years) and lowest at Cape Royds (3000 penguin pairs; 45 skua pairs). The log–log linear relationship (R2 = 0.98) between pairs of skuas and penguins was highly significant, and most historical estimates of skua and penguin numbers in the Ross Sea were within 95 % prediction intervals of the regression. Applying our regression model to current Adélie Penguin colony sizes at 23 western Ross Sea locations predicted that 4635 pairs of skuas now breed within 1000 m of penguin colonies in the Ross Island metapopulation (including Beaufort Island) and northern Victoria Land. We estimate, using published skua estimates for elsewhere in Antarctica, that the Ross Sea South Polar Skua population comprises ~50 % of the world total, although this may be an overestimate because of incomplete data elsewhere. To improve predictions and enable measurement of future skua population change, we recommend additional South Polar Skua surveys using consistent distance-sampling methods at penguin colonies of a range of sizes.

Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge

Science.gov (United States)

Konduru, Krishnamurthy; Shurtleff, Amy C.; Bradfute, Steven B.; Nakamura, Siham; Bavari, Sina; Kaplan, Gerardo

2016-01-01

Ebola virus (EBOV), a member of the Filoviridae that can cause severe hemorrhagic fever in humans and nonhuman primates, poses a significant threat to the public health. Currently, there are no licensed vaccines or therapeutics to prevent and treat EBOV infection. Several vaccines based on the EBOV glycoprotein (GP) are under development, including vectored, virus-like particles, and protein-based subunit vaccines. We previously demonstrated that a subunit vaccine containing the extracellular domain of the Ebola ebolavirus (EBOV) GP fused to the Fc fragment of human IgG1 (EBOVgp-Fc) protected mice against EBOV lethal challenge. Here, we show that the EBOVgp-Fc vaccine formulated with QS-21, alum, or polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) adjuvants induced strong humoral immune responses in guinea pigs. The vaccinated animals developed anti-GP total antibody titers of approximately 105−106 and neutralizing antibody titers of approximately 103 as assessed by a BSL-2 neutralization assay based on vesicular stomatitis virus (VSV) pseudotypes. The poly-ICLC formulated EBOVgp-Fc vaccine protected all the guinea pigs against EBOV lethal challenge performed under BSL-4 conditions whereas the same vaccine formulated with QS-21 or alum only induced partial protection. Vaccination with a mucin-deleted EBOVgp-Fc construct formulated with QS-21 adjuvant did not have a significant effect in anti-GP antibody levels and protection against EBOV lethal challenge compared to the full-length GP construct. The bulk of the humoral response induced by the EBOVgp-Fc vaccine was directed against epitopes outside the EBOV mucin region. Our findings indicate that different adjuvants can eliciting varying levels of protection against lethal EBOV challenge in guinea pigs vaccinated with EBOVgp-Fc, and suggest that levels of total anti-GP antibodies elicit by protein-based GP subunit vaccines do not correlate with protection. Our data further support

Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge.

Science.gov (United States)

Konduru, Krishnamurthy; Shurtleff, Amy C; Bradfute, Steven B; Nakamura, Siham; Bavari, Sina; Kaplan, Gerardo

2016-01-01

Ebola virus (EBOV), a member of the Filoviridae that can cause severe hemorrhagic fever in humans and nonhuman primates, poses a significant threat to the public health. Currently, there are no licensed vaccines or therapeutics to prevent and treat EBOV infection. Several vaccines based on the EBOV glycoprotein (GP) are under development, including vectored, virus-like particles, and protein-based subunit vaccines. We previously demonstrated that a subunit vaccine containing the extracellular domain of the Ebola ebolavirus (EBOV) GP fused to the Fc fragment of human IgG1 (EBOVgp-Fc) protected mice against EBOV lethal challenge. Here, we show that the EBOVgp-Fc vaccine formulated with QS-21, alum, or polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) adjuvants induced strong humoral immune responses in guinea pigs. The vaccinated animals developed anti-GP total antibody titers of approximately 105-106 and neutralizing antibody titers of approximately 103 as assessed by a BSL-2 neutralization assay based on vesicular stomatitis virus (VSV) pseudotypes. The poly-ICLC formulated EBOVgp-Fc vaccine protected all the guinea pigs against EBOV lethal challenge performed under BSL-4 conditions whereas the same vaccine formulated with QS-21 or alum only induced partial protection. Vaccination with a mucin-deleted EBOVgp-Fc construct formulated with QS-21 adjuvant did not have a significant effect in anti-GP antibody levels and protection against EBOV lethal challenge compared to the full-length GP construct. The bulk of the humoral response induced by the EBOVgp-Fc vaccine was directed against epitopes outside the EBOV mucin region. Our findings indicate that different adjuvants can eliciting varying levels of protection against lethal EBOV challenge in guinea pigs vaccinated with EBOVgp-Fc, and suggest that levels of total anti-GP antibodies elicit by protein-based GP subunit vaccines do not correlate with protection. Our data further support

Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge.

Directory of Open Access Journals (Sweden)

Krishnamurthy Konduru

Full Text Available Ebola virus (EBOV, a member of the Filoviridae that can cause severe hemorrhagic fever in humans and nonhuman primates, poses a significant threat to the public health. Currently, there are no licensed vaccines or therapeutics to prevent and treat EBOV infection. Several vaccines based on the EBOV glycoprotein (GP are under development, including vectored, virus-like particles, and protein-based subunit vaccines. We previously demonstrated that a subunit vaccine containing the extracellular domain of the Ebola ebolavirus (EBOV GP fused to the Fc fragment of human IgG1 (EBOVgp-Fc protected mice against EBOV lethal challenge. Here, we show that the EBOVgp-Fc vaccine formulated with QS-21, alum, or polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC adjuvants induced strong humoral immune responses in guinea pigs. The vaccinated animals developed anti-GP total antibody titers of approximately 105-106 and neutralizing antibody titers of approximately 103 as assessed by a BSL-2 neutralization assay based on vesicular stomatitis virus (VSV pseudotypes. The poly-ICLC formulated EBOVgp-Fc vaccine protected all the guinea pigs against EBOV lethal challenge performed under BSL-4 conditions whereas the same vaccine formulated with QS-21 or alum only induced partial protection. Vaccination with a mucin-deleted EBOVgp-Fc construct formulated with QS-21 adjuvant did not have a significant effect in anti-GP antibody levels and protection against EBOV lethal challenge compared to the full-length GP construct. The bulk of the humoral response induced by the EBOVgp-Fc vaccine was directed against epitopes outside the EBOV mucin region. Our findings indicate that different adjuvants can eliciting varying levels of protection against lethal EBOV challenge in guinea pigs vaccinated with EBOVgp-Fc, and suggest that levels of total anti-GP antibodies elicit by protein-based GP subunit vaccines do not correlate with protection. Our data

Distribution and ventilation of water masses in the western Ross Sea inferred from CFC measurements

Science.gov (United States)

Rivaro, Paola; Ianni, Carmela; Magi, Emanuele; Massolo, Serena; Budillon, Giorgio; Smethie, William M.

2015-03-01

During the CLIMA Project (R.V. Italica cruise PNRA XVI, January-February 2001), hydrographic and chlorofluorocarbons (CFCs) observations were obtained, particularly in the western Ross Sea. Their distribution demonstrated water mass structure and ventilation processes in the investigated areas. In the surface waters (AASW) the CFC saturation levels varied spatially: CFCs were undersaturated in all the areas (range from 80 to 90%), with the exception of few stations sampled near Ross Island. In particular, the Terra Nova Bay polynya, where high salinity shelf water (HSSW) is produced, was a low-saturated surface area (74%) with respect to CFCs. Throughout most of the shelf area, the presence of modified circumpolar deep water (MCDW) was reflected in a mid-depth CFC concentration minima. Beneath the MCDW, CFC concentrations generally increased in the shelf waters towards the seafloor. We estimated that the corresponding CFCs saturation level in the source water region for HSSW was about 68-70%. Waters with high CFC concentrations were detected in the western Ross Sea on the down slope side of the Drygalski Trough, indicating that AABW was being supplied to the deep Antarctic Basin. Estimates of ventilation ages depend strongly on the saturation levels. We calculated ventilation ages using the saturation level calibrated tracer ratio, CFC11/CFC12. We deduced a mean residence time of the shelf waters of about 6-7 years between the western Ross Sea source and the shelf break.

Characteristics and processing of seismic data collected on thick, floating ice: Results from the Ross Ice Shelf, Antarctica

Science.gov (United States)

Beaudoin, Bruce C.; ten Brink, Uri S.; Stern, Tim A.

1992-01-01

Coincident reflection and refraction data, collected in the austral summer of 1988/89 by Stanford University and the Geophysical Division of the Department of Scientific and Industrial Research, New Zealand, imaged the crust beneath the Ross Ice Shelf, Antarctica. The Ross Ice Shelf is a unique acquisition environment for seismic reflection profiling because of its thick, floating ice cover. The ice shelf velocity structure is multilayered with a high velocity‐gradient firn layer constituting the upper 50 to 100 m. This near surface firn layer influences the data character by amplifying and frequency modulating the incoming wavefield. In addition, the ice‐water column introduces pervasive, high energy seafloor, intra‐ice, and intra‐water multiples that have moveout velocities similar to the expected subseafloor primary velocities. Successful removal of these high energy multiples relies on predictive deconvolution, inverse velocity stack filtering, and frequency filtering. Removal of the multiples reveals a faulted, sedimentary wedge which is truncated at or near the seafloor. Beneath this wedge the reflection character is diffractive to a two‐way traveltime of ∼7.2 s. At this time, a prominent reflection is evident on the southeast end of the reflection profile. This reflection is interpreted as Moho indicating that the crust is ∼21-km thick beneath the profile. These results provide seismic evidence that the extensional features observed in the Ross Sea region of the Ross Embayment extend beneath the Ross Ice Shelf.

Dilatation and Dysfunction of the Neo-aortic Root and in 76 Patients After the Ross Procedure.

Science.gov (United States)

Zimmermann, Corina A; Weber, Roland; Greutmann, Matthias; Dave, Hitendu; Müller, Christoph; Prêtre, René; Seifert, Burkhardt; Buechel, Emanuela Valsangiacomo; Kretschmar, Oliver; Attenhofer Jost, Christine H

2016-08-01

Pulmonary autograft replacement (Ross procedure) is used as an alternative to prosthetic aortic valve replacement patients with aortic valve disease. There are limited data on incidence and risk factors for dilatation and dysfunction of the neo-aortic after the Ross procedure. Ross procedure was performed in 100 patients at our institution between 1993 and 2011. In 76 patients, complete follow-up data were available. Their median age at surgery was 16 (0.4-58) years (76 % males; 95 % with congenital aortic valve disease). Median follow-up duration was 5.2 years (0.3-16.0 years). We analyzed their clinical and echocardiographic follow-up to identify possible risk factors for neo-aortic root dilatation and dysfunction. Ross procedure included reduction plasty of the native ascending aorta in 25 % of patients. During follow-up, 21 patients (28 %) developed neo-aortic root dilatation, 38 patients (50 %) dilatation oft the native ascending aorta and 7 patients (9 %) at least moderate neo-aortic regurgitation. Univariate risk factors for neo-aortic root dilatation were preoperative aortic regurgitation (p = 0.04), concomitant reduction plasty of the ascending aorta (p = 0.009) and a longer duration of follow-up (p = 0.005). Younger age at surgery was associated with dilatation of the ascending aorta (p = 0.03). Reoperation on the neo-aortic root because of severe dilatation was necessary in 6 patients (8 %), where 2 patients had at least moderate neo-aortic root regurgitation. Neo-aortic root and aortic dilatation are common after the Ross procedure. This is often combined with neo-aortic valve dysfunction. Close follow-up of these patients is mandatory.

Percutaneous valved stent repair of a failed homograft: implications for the Ross procedure.

Science.gov (United States)

Pretorius, Victor; Jones, Alan; Taylor, Dylan; Coe, Yashu; Ross, David B

2008-08-01

A case of percutaneous pulmonary valve implantation following a failed homograft in the pulmonary position is reported. A 16-year-old boy developed infective endocarditis of his pulmonary homograft, which was implanted four years earlier during a Ross procedure for congenital aortic stenosis. Following successful medical therapy, the boy was symptomatic due to pulmonary stenosis and regurgitation. A 22 mm Melody valve (Medtronic, USA) was successfully implanted percutaneously. His symptoms resolved and he was discharged home one day after the procedure. Echocardiography at the six-month follow-up demonstrated a normally functioning pulmonary valve. Percutaneous pulmonary valve replacement may make the Ross procedure a more attractive option for patients with aortic stenosis, particularly in the pediatric population.

Ross In Situ Uranium Recovery Project NESHAP Subpart W Construction Approval

Science.gov (United States)

On May 5, 2015, EPA issued a Construction Approval under the National Emission Standards for Hazardous Air Pollutants (NESHAPs) at 40 CFR Part 61, subpart W, to Strata Energy, Inc., for their Ross In Situ Recovery (ISR) Uranium Project in Crook County, WY.

Photographer: Digital Telepresence: Dr Murial Ross's Virtual Reality Application for Neuroscience

Science.gov (United States)

1995-01-01

Photographer: Digital Telepresence: Dr Murial Ross's Virtual Reality Application for Neuroscience Research Biocomputation. To study human disorders of balance and space motion sickness. Shown here is a 3D reconstruction of a nerve ending in inner ear, nature's wiring of balance organs.

Ezrin interacts with the SARS coronavirus Spike protein and restrains infection at the entry stage.

Directory of Open Access Journals (Sweden)

Jean Kaoru Millet

Full Text Available BACKGROUND: Entry of Severe Acute Respiratory Syndrome coronavirus (SARS-CoV and its envelope fusion with host cell membrane are controlled by a series of complex molecular mechanisms, largely dependent on the viral envelope glycoprotein Spike (S. There are still many unknowns on the implication of cellular factors that regulate the entry process. METHODOLOGY/PRINCIPAL FINDINGS: We performed a yeast two-hybrid screen using as bait the carboxy-terminal endodomain of S, which faces the cytosol during and after opening of the fusion pore at early stages of the virus life cycle. Here we show that the ezrin membrane-actin linker interacts with S endodomain through the F1 lobe of its FERM domain and that both the eight carboxy-terminal amino-acids and a membrane-proximal cysteine cluster of S endodomain are important for this interaction in vitro. Interestingly, we found that ezrin is present at the site of entry of S-pseudotyped lentiviral particles in Vero E6 cells. Targeting ezrin function by small interfering RNA increased S-mediated entry of pseudotyped particles in epithelial cells. Furthermore, deletion of the eight carboxy-terminal amino acids of S enhanced S-pseudotyped particles infection. Expression of the ezrin dominant negative FERM domain enhanced cell susceptibility to infection by SARS-CoV and S-pseudotyped particles and potentiated S-dependent membrane fusion. CONCLUSIONS/SIGNIFICANCE: Ezrin interacts with SARS-CoV S endodomain and limits virus entry and fusion. Our data present a novel mechanism involving a cellular factor in the regulation of S-dependent early events of infection.

Umírání a smrt v pojetí Elisabeth Kübler-Ross

OpenAIRE

TOTHOVÁ, Eva

2013-01-01

This work is focused on dying and exitus in understanding of Elisabeth Kübler-Ross. How did this unique woman feel the end of life, what was the sense and the main contens of her life. Elisabeth Kübler-Ross gave most of her life to help incurables people, their families and friends. During her work she described and defined five periods of confirmed illneses´ incidence. She was exemplary hearer, friend, every patient was her master. She felt a possibility to be with deathwards, to listen and ...

Preliminary results on the petrology and fluid inclusions of the Rossing uraniferous alaskites

International Nuclear Information System (INIS)

Cuney, M.

1980-01-01

Petrography, radioactivity distribution and fluid inclusions have been studied in three samples of the Rossing alaskites from the S.H. anomaly outside of the Rossing deposit. After the crystallization of the alaskitic magma which involved oligoclase, biotite, orthoclase and quartz, a deuteric alteration produced a quartz, microcline, albite, muscovite, calcite plus minus chlorite mineral association similar to those observed in several French uraniferous granites. Uranium redistribution occurred during this alteration. From fluid inclusions data, the pressure at the time of the intrusion is estimated to have been at least 6kbar for a temperature of 625 degrees Celsius. Reaction of the magma with the marbles of the Rossing formation led to the boiling of the magma by an increase in the CO 2 partial pressure as well as to its crystallization. Immiscibility between a dense saline (more than 30 per cent NaCl) and CO 2 -rich fluid is proposed to have occurred simultaneously or after the fluid oversaturation of the magma. Part of uraninite is formed at the magmatic stage, another part crystallized from the magmatic fluids in the biotite-rich selvages of the alaskites or in the uraninite-fluorite veins. Uraninite crystallization appears to be mainly controlled by the oxygen fugacity prevailing in the magma and in the surrounding rocks

STS-61B Astronaut Ross During ACCESS Extravehicular Activity

Science.gov (United States)

1985-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, VA and the Marshall Space Flight Center (MSFC), ACCESS and EASE were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo, astronaut Ross was working on the ACCESS experiment during an Extravehicular Activity (EVA). The primary objective of this experiment was to test the ACCESS structural assembly concept for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Problems and Possible Solutions Concerning Radiocarbon Dating of Surface Marine Sediments, Ross Sea, Antarctica

Science.gov (United States)

Andrews, John T.; Domack, Eugene W.; Cunningham, Wendy L.; Leventer, Amy; Licht, Kathy J.; Jull, A. J. Timothy; DeMaster, David J.; Jennings, Anne E.

1999-09-01

Radiocarbon accelerator mass spectrometric (AMS) dates on the acid-insoluble fraction from 38 core tops from the western Ross Sea, Antarctica, are used to address these questions: (1) What are the apparent ages of sediments at or close to the present sediment/water interface? (2) Is there a statistically significant pattern to the spatial distribution of core top ages? and (3) Is there a "correction factor" that can be applied to these age determinations to obtain the best possible Holocene (downcore) chronologies? Ages of core top sediments range from 2000 to 21,000 14C yr B.P. Some "old" core top dates are from piston cores and probably represent the loss of sediment during the coring process, but some core top samples >6000 14C yr B.P. may represent little or no Holocene deposition. Four possible sources of variability in dates ≤6000 14C yr B.P. (n = 28) are associated with (1) different sample preparation methods, (2) different sediment recovery systems, (3) different geographic regions, and (4) within-sample lateral age variability. Statistical analysis on an a posteriori design indicates that geographic area is the major cause of variability; there is a difference in mean surface sediment age of nearly 2000 yr between sites in the western Ross Sea and sites east of Ross Bank in south-central Ross Sea. The systematic variability in surface age between areas may be attributed to: (a) variable sediment accumulation rates (SAR) (surface age is inversely related to SAR), (b) differences in the percentage of reworked (dead) carbon between each area, and/or (c) differences in the CO2 exchange between the ocean and the atmosphere.

76 FR 77684 - Establishment of the Fort Ross-Seaview Viticultural Area

Science.gov (United States)

2011-12-14

...; Treasury decision. SUMMARY: This Treasury decision establishes the 27,500-acre ``Fort Ross-Seaview... may purchase. DATES: Effective Date: January 13, 2012. FOR FURTHER INFORMATION CONTACT: Elisabeth C... may purchase. Establishment of a viticultural area is neither an approval nor an endorsement by TTB of...

On the outflow of dense water from the Weddell and Ross Seas in OCCAM model

Directory of Open Access Journals (Sweden)

R. Kerr

2012-06-01

Full Text Available We describe the seasonal and interannual variability of volume transports in the Weddell and Ross Seas using the 1/12° 20-yr simulation of the OCCAM global ocean general circulation model. The average simulated full-depth cumulative volume transports were 28.5 ± 2.9 Sv (1 Sv ≡ 10⁶ m³ s⁻¹ and 13.4 ± 5.2 Sv, across the main export regions of the Weddell and Ross Seas, respectively. The values of mean outflow of Antarctic Bottom Water (AABW (defined by neutral density γⁿ ≥ 28.27 kg m⁻³ from the Weddell and Ross Seas of 10.6 ± 3.1 Sv and 0.5 ± 0.7 Sv, respectively, agree with the range reported in historical observational studies. The export of Weddell Sea dense water in OCCAM is primarily determined by the strength of the Weddell Gyre. Variability in AABW export is predominantly at periods of ~1 yr and 2–4 yr.

The hydroxyapatite-binding regions of a rat salivary glycoprotein.

Science.gov (United States)

Embery, G; Green, D R

1989-09-01

The regions of a salivary sulphated glycoprotein which are involved in its attachment to hydroxyapatite (Biogel HTP) have been characterised. The sulphated glycoprotein, a 35S-labelled preparation from mixed palatal and buccal minor gland secretions of the rat was bound onto hydroxyapatite and the resultant glycoprotein-hydroxyapatite complex was sequentially digested with pronase E and alpha-L-fucosidase, a treatment which released 86.8% +/- 1.7% of the radioactivity of the initially bound glycoprotein. The fragments which remained attached to the hydroxyapatite after enzymic digestion were fractionated on Sephadex G-25 and analysed for carbohydrate and amino acid components. A range of amino acids were detected which could reflect both glycosylated and non-glycosylated-binding regions. Sialic acid, although considered to be involved in the attachment process was not detected in any of the fragments remaining after enzymic digestion, a finding which provides indirect evidence that the enzymically liberated products do not subsequently re-attach to the hydroxyapatite surface. The notable feature of the fractions with average Mr estimated at 1000 or less is the high proportion of N-acetylhexosamine and N-acetylgalactosamine. It is apparent that the hexosamine residues, which normally bear the ester sulphate moieties of sulphated glycoproteins, play an important role in the attachment of sulphated glycoproteins to hydroxyapatite.

Competition and Concentration in Bangladeshi Banking Sector: An Application of Panzar-Rosse Model

Directory of Open Access Journals (Sweden)

Md. Anwar Hossain Repon

2016-01-01

Full Text Available The purpose of this paper is to investigate the market structure and degree of concentration of Bangladeshi banking industry. The study measured market concentration by using widely recognized measures like k-bank concentration ratio and Herfindahl-Hirchman Index (HHI. It evaluates market structure by applying Panzar-Rosse Model over 8 years period from 2006 to 2013. The result of concentration measures indicates a decreasing trend and low level of market concentration in Bangladeshi banking industry over the sample period. The panzer-Rosse “H-Statistic” suggests that banks in Bangladesh are operating under monopolistic competition. Present paper contributes to a burgeoning literature on banking competition that has evolved significantly over the past periods on a developing country perspective like Bangladesh.

Isolation of allergenically active glycoprotein from Prosopis juliflora pollen.

Science.gov (United States)

Thakur, I S

1989-03-01

An allergenically active glycoprotein was homogeneously isolated from the aqueous extract of Prosopis juliflora pollen by ConA-Sepharose affinity chromatography. The molecular weight of this glycoprotein was 20,000 dalton, determined by gel filtration and SDS-PAGE. This fraction showed a total carbohydrate concentration of 25%. The purified glycoprotein revealed immunochemically most antigenic or allergenic and demonstrated homogeneous after reaction with P. juliflora pollen antiserum, characterized by gel diffusion, Immunoelectrophoresis and Radioallergosorbent test.

A Helping Hand in the Frederick Community—Ross Smith | Poster

Science.gov (United States)

By day, Ross Smith is the compliance and security officer for Data Management Services, Inc., assigned to the National Cancer Institute (NCI) at Frederick. His role is to ensure the secure operation of in-house computer systems, servers, and network connections. But in his spare time, Smith is also a volunteer firefighter and emergency medical technician (EMT).

Intracellular localization of hydroxyproline-rich glycoprotein biosynthesis

International Nuclear Information System (INIS)

Robinson, D.G.; Andreae, M.; Glas, A.R.; Sauer, A.

1984-01-01

The structural proteins of plant cell walls are glycoproteins characterized by O-glucosidic linkages to hydroxyproline or serine. Proline, not hydroxyproline, is the translatable amino acid in hydroxyproline-rich glycoproteins (HRGP). Hydroxylation and arabinosylation of proline are sequential, post-translational events. Because of this, there is no a priori reason for expecting HRGP synthesis to follow the well-established route for secretory and plasma membrane (PM) glycoproteins, i.e., from endoplasmic reticulum (ER) via the Golgi apparatus (GA) to the PM. In this paper, two plausible alternatives for HRGO secretion are examined. Because a feature of the majority of dicotyledons is overlapping GA and PM regions in sucrose density gradients, the authors have used two monocotyledonous systems to determine the distribution of HRGP and enzyme activity

Impact of pregnancy on autograft dilatation and aortic valve function following the Ross procedure.

Science.gov (United States)

Carvajal, Horacio G; Lindley, Kathryn J; Shah, Trupti; Brar, Anoop K; Barger, Philip M; Billadello, Joseph J; Eghtesady, Pirooz

2018-03-01

The effects of pregnancy on autograft dilatation and neoaortic valve function in patients with a Ross procedure have not been studied. We sought to evaluate the effect of pregnancy on autograft dilatation and valve function in these patients with the goal of determining whether pregnancy is safe after the Ross procedure. A retrospective chart review of female patients who underwent a Ross procedure was conducted. Medical records for 51 patients were reviewed. Among the 33 patients who met inclusion criteria, 11 became pregnant after surgery and 22 did not. Echocardiographic reports were used to record aortic root diameter and aortic insufficiency before, during, and after pregnancy. Patient's charts were reviewed for reinterventions and complications. Primary endpoints included reinterventions, aortic root dilation of ≥5 cm, aortic insufficiency degree ≥ moderate, and death. There were 18 pregnancies carried beyond 20 weeks in 11 patients. There was no significant difference in aortic root diameter between nulliparous patients and parous patients prior to their first pregnancy (3.53 ± 0.44 vs 3.57 ± 0.69 cm, P = .74). There was no significant change in aortic root diameter after first pregnancy (3.7 ± 0.4 cm, P = .056) although there was significant dilatation after the second (4.3 ± 0.7 cm, P = .009) and third (4.5 ± 0.7 cm, P = .009) pregnancies. Freedom from combined endpoints was significantly higher for patients in the pregnancy group than those in the nonpregnancy group (P = .002). Pregnancy was not associated with significantly increased adverse events in patients following the Ross procedure. Special care should be taken after the first pregnancy, as multiparity may lead to increased neoaortic dilatation. © 2017 Wiley Periodicals, Inc.

Analysis of glycoprotein-derived oligosaccharides in glycoproteins detected on two-dimensional gel by capillary electrophoresis using on-line concentration method.

Science.gov (United States)

Kamoda, Satoru; Nakanishi, Yasuharu; Kinoshita, Mitsuhiro; Ishikawa, Rika; Kakehi, Kazuaki

2006-02-17

Capillary electrophoresis (CE) is an effective tool to analyze carbohydrate mixture derived from glycoproteins with high resolution. However, CE has a disadvantage that a few nanoliters of a sample solution are injected to a narrow capillary. Therefore, we have to prepare a sample solution of high concentration for CE analysis. In the present study, we applied head column field-amplified sample stacking method to the analysis of N-linked oligosaccharides derived from glycoprotein separated by two-dimensional gel electrophoresis. Model studies demonstrated that we achieved 60-360 times concentration effect on the analysis of carbohydrate chains labeled with 3-aminobenzoic acid (3-AA). The method was applied to the analysis of N-linked oligosaccharides from glycoproteins separated and detected on PAGE gel. Heterogeneity of alpha1-acid glycoprotein (AGP), i.e. glycoforms, was examined by 2D-PAGE and N-linked oligosaccharides were released by in-gel digestion with PNGase F. The released oligosaccharides were derivatized with 3-AA and analyzed by CE. The results showed that glycoforms having lower pI values contained a larger amount of tetra- and tri-antennary oligosaccharides. In contrast, glycoforms having higher pI values contained bi-antennary oligosaccharides abundantly. The result clearly indicated that the spot of a glycoprotein glycoform detected by Coomassie brilliant blue staining on 2D-PAGE gel is sufficient for quantitative profiling of oligosaccharides.

Early and mid-term results of autograft rescue by Ross reversal: A one-valve disease need not become a two-valve disease.

Science.gov (United States)

Hussain, Syed T; Majdalany, David S; Dunn, Aaron; Stewart, Robert D; Najm, Hani K; Svensson, Lars G; Houghtaling, Penny L; Blackstone, Eugene H; Pettersson, Gösta B

2018-02-01

Risk of reoperation and loss of a second native valve are major drawbacks of the Ross operation. Rather than discarding the failed autograft, it can be placed back into the native pulmonary position by "Ross reversal." We review our early and mid-term results with this operation. From 2006 to 2017, 39 patients underwent reoperation for autograft dysfunction. The autograft was successfully rescued in 35 patients: by Ross reversal in 30, David procedure in 4, and autograft repair in 1. Medical records were reviewed for patient characteristics (mean age was 46 ± 13 years, range 18-67 years, and 23 were male), previous operations, indications for reoperation, hospital outcomes, and echocardiographic findings for the 30 patients undergoing successful Ross reversal. Follow-up was 4.1 ± 3.5 years (range 7 months-11 years). Median interval between the original Ross procedure and Ross reversal was 12 years (range 5-19 years). Eight patients also had absolute indications for replacement of the pulmonary allograft. There was no operative mortality. One patient required reoperation for bleeding. Another had an abdominal aorta injury from use of an endoballoon clamp. There was no other major postoperative morbidity, and median postoperative hospital stay was 7.2 days (range 4-41 days). No patient required reoperation during follow-up. Twenty-four patients had acceptable pulmonary valve function, and 6 had clinically well-tolerated moderate or severe pulmonary regurgitation. Ross reversal can be performed with low morbidity and acceptable pulmonary valve function, reducing patient risk of losing 2 native valves when the autograft fails in the aortic position. Copyright © 2017 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.

Unusual biogenic calcite structures in two shallow lakes, James Ross Island, Antarctica

Czech Academy of Sciences Publication Activity Database

Elster, Josef; Nedbalová, Linda; Vodrážka, R.; Láska, K.; Haloda, J.; Komárek, Jiří

2016-01-01

Roč. 13, č. 2 (2016), s. 535-549 ISSN 1726-4170 R&D Projects: GA MŠk ME 945 Institutional support: RVO:67985939 Keywords : James Ross Island * cyanobacteria * microalgae Subject RIV: EH - Ecology, Behaviour Impact factor: 3.851, year: 2016

Forced copulation results in few extrapair fertilizations in Ross's and lesser snow geese

Science.gov (United States)

Dunn, P.O.; Afton, A.D.; Gloutney, M.L.; Alisauskas, R.T.

1999-01-01

Extrapair paternity varies from 0 to over 70% of young among various populations of birds. Comparative studies have suggested that this variation is related to nesting density, breeding synchrony and the proportion of extrapair copulations. We used minisatellite DNA fingerprinting to examine levels of extrapair paternity in Ross's geese, Chen rossi, and lesser snow geese, C. caerulescens c. (hereafter snow geese) nesting in the largest known goose colony in the world. These geese have one of the highest known percentages of extrapair copulation (46-56% of all attempted copulations), and all of these appeared to be forced. Among all successful copulations, 33 and 38% were extrapair in Ross's and snow geese, respectively. Despite the high percentage of extrapair copulations, extrapair paternity was low in both Ross's and snow geese (2-5% of young). Extrapair paternity was not related to nest density in either species. However, in snow geese, extrapair paternity was more likely to occur in nests of females that nested asynchronously, either early or late in the season. This is one of a few reported examples of a negative relationship between extrapair paternity and breeding synchrony. Extrapair young also tended to come from eggs laid later in the clutch. Although forced extrapair copulations appear to be a relatively inefficient reproductive tactic for males, they may provide a reproductive advantage for some males.

Humanizing recombinant glycoproteins from Chinese hamster ovary cells

DEFF Research Database (Denmark)

Hansen, Anders Holmgaard; Amann, Thomas; Kol, Stefan

With new tools for gene-editing like zinc-fingers, TALENS and CRISPR, it is now feasible totailor-make the N-Glycoforms for therapeutic glycoproteins that have previously been almost impossible. We here demonstrate a case of humanizing a recombinant human glycoprotein that in Wild type (WT) Chinese...

Multichannel Seismic Reflection Data - SCAR - Ross Sea - 1987, SDLS CD-ROM vol 13

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data recorded during the 1987 field season in the Ross Sea, Antarctica, by SEVMORGEOLOGIA, RUSSIA. The...

Multichannel Seismic Reflection Data - SCAR - Ross Sea - 1980, SDLS CD-ROM vol 3

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data from four lines recorded during 1980 in the Ross Sea, Antarctica, by the Bundesanstalt fur...

Multichannel Seismic Reflection Data - SCAR - Ross Sea - 1980, SDLS, CD-ROM vol 4

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data from seven lines recorded during 1980 in the Ross Sea, Antarctica, by the Bundesanstalt fur...

Multichannel Seismic Reflection Data - SCAR - Ross Sea - 1989, SDLS CD-ROM vol 14

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data recorded during 1989 field season in the Ross Sea, Antarctica, by the SEVMORGEOLOGIA, RUSSIS. The...

Multichannel Seismic Reflection Data - SCAR - Ross Sea 1980, SDLS, CD-ROM vol 5

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data from eleven lines recorded during 1980 in the Ross Sea, Antarctica, by the Bundesanstalt fur...

Climate change impacts on southern Ross Sea phytoplankton composition, productivity, and export

Science.gov (United States)

Kaufman, Daniel E.; Friedrichs, Marjorie A. M.; Smith, Walker O.; Hofmann, Eileen E.; Dinniman, Michael S.; Hemmings, John C. P.

2017-03-01

The Ross Sea, a highly productive region of the Southern Ocean, is expected to experience warming during the next century along with reduced summer sea ice concentrations and shallower mixed layers. This study investigates how these climatic changes may alter phytoplankton assemblage composition, primary productivity, and export. Glider measurements are used to force a one-dimensional biogeochemical model, which includes diatoms and both solitary and colonial forms of Phaeocystis antarctica. Model performance is evaluated with glider observations, and experiments are conducted using projections of physical drivers for mid-21st and late-21st century. These scenarios reveal a 5% increase in primary productivity by midcentury and 14% by late-century and a proportional increase in carbon export, which remains approximately 18% of primary production. In addition, scenario results indicate diatom biomass increases while P. antarctica biomass decreases in the first half of the 21st century. In the second half of the century, diatom biomass remains relatively constant and P. antarctica biomass increases. Additional scenarios examining the independent contributions of expected future changes (temperature, mixed layer depth, irradiance, and surface iron inputs from melting ice) demonstrate that earlier availability of low light due to reduction of sea ice early in the growing season is the primary driver of productivity increases over the next century; shallower mixed layer depths additionally contribute to changes of assemblage composition and export. This study further demonstrates how glider data can be effectively used to facilitate model development and simulation, and inform interpretation of biogeochemical observations in the context of climate change.Plain Language SummaryUnderstanding how the global ocean responds to climate change requires knowing the natural behavior of individual regions and anticipating how future changes will affect each region differently. It

Determination of P-Glycoprotein Expression by Flow Cytometry in Hematological Malignancies

Directory of Open Access Journals (Sweden)

Berkay Saraymen

2016-03-01

Full Text Available Objective: Determination the expression of P-glycoprotein is especially problematic for normal tissues because immuno­logical methods are limited in terms of sensitivity. We aimed to determine the expression of P-glycoprotein and CD34 by flow cytometry, and to evaluate the level of expression of P-glycoprotein and CD34 with unresponsive to treatment in pa­tients diagnosed with hematologic malignancy. Methods: Our study included fifty patients diagnosed with acute myeloblastic leukemia and acute lymphoblastic leuke­mia, and twenty healthy controls who were admitted to Erci­yes University Hematology-Oncology Hospital. The suspend­ed cells from bone marrow samples of patients and the pe­ripheral blood samples of healthy people were marked with P-glycoprotein phycoerythrin and CD34 FITC or PerCP Cy 5.5; and then surface expression was measured by means of flow cytometry. Results: In 6 of 30 acute myeloblastic leukemia patients P-glycoprotein and CD34 expression, in 6 of 20 acute lympho­blastic leukemia patients P-glycoprotein, in 5 of them CD34 expression were determined. A significant relation between P-glycoprotein and CD34 expressions in acute myeloblas­tic leukemia and acute lymphoblastic leukemia bone marrow samples was reported. Conclusion: Our data indicate that flow cytometry is more reliable, precise and faster than molecular methods for mea­suring P-glycoprotein expression and suggests the pos­sibility of a significant relationship between P-glycoprotein and CD34 expressions in acute myeloblastic leukemia and acute lymphoblastic leukemia bone marrow samples. The blast cells expressing CD34 on their surface along with P-glycoprotein simultaneously show that multi drug resistance 1 gene is mostly active in immature cells.

Validation of an automated system for aliquoting of HIV-1 Env-pseudotyped virus stocks.

Science.gov (United States)

Schultz, Anke; Germann, Anja; Fuss, Martina; Sarzotti-Kelsoe, Marcella; Ozaki, Daniel A; Montefiori, David C; Zimmermann, Heiko; von Briesen, Hagen

2018-01-01

The standardized assessments of HIV-specific immune responses are of main interest in the preclinical and clinical stage of HIV-1 vaccine development. In this regard, HIV-1 Env-pseudotyped viruses play a central role for the evaluation of neutralizing antibody profiles and are produced according to Good Clinical Laboratory Practice- (GCLP-) compliant manual and automated procedures. To further improve and complete the automated production cycle an automated system for aliquoting HIV-1 pseudovirus stocks has been implemented. The automation platform consists of a modified Tecan-based system including a robot platform for handling racks containing 48 cryovials, a Decapper, a tubing pump and a safety device consisting of ultrasound sensors for online liquid level detection of each individual cryovial. With the aim to aliquot the HIV-1 pseudoviruses in an automated manner under GCLP-compliant conditions a validation plan was developed where the acceptance criteria-accuracy, precision as well as the specificity and robustness-were defined and summarized. By passing the validation experiments described in this article the automated system for aliquoting has been successfully validated. This allows the standardized and operator independent distribution of small-scale and bulk amounts of HIV-1 pseudovirus stocks with a precise and reproducible outcome to support upcoming clinical vaccine trials.

Glycoproteins of mouse vaginal epithelium: differential expression related to estrous cyclicity

DEFF Research Database (Denmark)

Horvat, B; Multhaupt, H A; Damjanov, I

1993-01-01

We used lectin overlay blotting and SDS-PAGE to analyze the estrous cycle-specific expression of mouse vaginal epithelial glycoproteins. Seven lectins chosen for their differential carbohydrate-binding specificity revealed 15 glycoproteins that showed cycle-related expression. Each lectin had...... in proestrus, coincident with the transformation of two superficial layers of vaginal squamous epithelium into mucinous cuboidal cells. Electron microscopic lectin histochemistry revealed the glycoproteins in the mucinous granules of surface cuboidal cells and in the lumen of the vagina. Our results illustrate...... the complexity of glycoconjugate synthesis in mouse vagina and reveal the distinct cycle-specific patterns of individual glycoprotein expression. These cyclic glycoproteins could serve as vaginal biochemical markers for the specific phases of the estrous cycle....

Mid-term Outcome of 100 Consecutive Ross Procedures: Excellent Survival, But Yet to Be a Cure.

Science.gov (United States)

Zimmermann, Corina; Attenhofer Jost, Christine; Prêtre, René; Mueller, Christoph; Greutmann, Matthias; Seifert, Burkhardt; Valsangiacomo Büchel, Emanuela; Kretschmar, Oliver; Dave, Hitendu Hasmukhlal; Weber, Roland

2018-03-01

The Ross procedure offers excellent short-term outcome but the long-term durability is under debate. Reinterventions and follow-up of 100 consecutive patients undergoing Ross Procedure at our centre (1993-2011) were analysed. Follow-up was available for 96 patients (97%) with a median duration of 5.3 (0.1-17.1) years. Median age of the patient cohort was 15.2 (0.04-58.4) years with 76 males. 93% had underlying congenital aortic stenosis. Root replacement technique was applied in all. The most common valved conduits used for reconstruction of the right ventricular outflow tract were homografts (66 patients) and bovine jugular vein (Contegra R ) graft (31 patients). Additional procedures included Ross-Konno procedure (14%), resection of subaortic stenosis/myectomy (11%) and reduction plasty of the ascending aorta (25%). One patient died within the first 30 days (1%). Late deaths occurred in 4 patients (4%) 0.5-4.5 years postoperatively: causes included pulmonary hypertension due to endocardial fibroelastosis (2), subarachnoid haemorrhage (1) and sudden cardiac death (1). Five-year survival was 93.6 (95% CI 88.1-99.1)%. Moderate or severe aortic (autograft) regurgitation needing reoperation occurred in 8 patients with a 5-year freedom from autograft reoperation of 98.5 (95.6-100)%. Five-year freedom from reintervention (surgery or catheter based) on the right ventricular outflow tract conduit was 91.5 (85.5-96.5)%. Univariate predictors of this reinterventions were smaller graft size (p = 0.03) and use of a Contegra R graft (p = 0.04). Ross procedure can be performed with low mortality and good survival in the long term. Most of the reinterventions are related to the neo-right ventricular outflow tract and may be partly attributed to the lack of growth. While the Ross Procedure remains an invaluable option for aortic valve disease in children, new solutions for the neo-pulmonary valve as well as for the less often occurring problems on the autograft are

Sources of iron in the Ross Sea Polynya in early summer

NARCIS (Netherlands)

Gerringa, L. J. A.; Laan, P.; van Dijken, G. L.; van Haren, H.; De Baar, H. J. W.; Arrigo, K. R.; Alderkamp, A. -C.

2015-01-01

Dissolved Fe (DFe) was measured in the Ross Sea Polynya (RSP), Antarctica, during a GEOTRACES cruise between 20 December 2013 and 5 January 2014. DFe was measured over the full water column with special emphasis on samples near the seafloor. In the upper mixed layer, DFe was very low everywhere (

Multichannel Seismic Reflection Data - SCAR, Ross Sea - 1982-1983, CD-ROM vol 9

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data from fourteen lines recorded during 1982 in the Ross Sea and Wilkes Island, Antarctica, by the...

Multichannel Seismic Reflection Data - SCAR - Ross Sea 1982, SDLS CD-ROM vol 12

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data from seven lines recorded during 1982 in the Ross Sea, Antarctica, by the Institut Francais du...

Estimation of the banking sector competition in the CEE countries: The Panzar-Rosse approach

Directory of Open Access Journals (Sweden)

Arben Mustafa

2017-12-01

Full Text Available Competitive conditions in the banking sectors in the light of transition process in the Central and Eastern Europe countries (CEE has been subject of interest for researchers and policy-makers, especially upon entry of foreign banks and concentrated markets. In this paper the Panzar-Rosse approach is used to assess competition of banking sectors in CEE. By using General Method of Moments on the panel data for 300 banks for the period 1999-2009, empirical evidence indicates that banks operating in the CEE exert monopoly behaviour. From the methodological point of view, the findings suggest that using a price equation or controlling for total assets in a Panzar-Rosse approach produces upwards biased and invalid estimates of the H-statistic. Therefore, further support is provided for maintaining a reducedform revenue equation when estimating a Panzar-Rosse model. The findings also suggest that banks operating in the non-EU countries of the CEE region have exerted stronger market power compared to the banks operating in the EU countries of this region. Policy-makers should improve competitive conditions and general ‘enabling’ environment for the banking sectors, especially the non-EU countries of the CEE region, while balancing competition-stability trade-off.

Herpesvirus glycoproteins undergo multiple antigenic changes before membrane fusion.

Directory of Open Access Journals (Sweden)

Daniel L Glauser

Full Text Available Herpesvirus entry is a complicated process involving multiple virion glycoproteins and culminating in membrane fusion. Glycoprotein conformation changes are likely to play key roles. Studies of recombinant glycoproteins have revealed some structural features of the virion fusion machinery. However, how the virion glycoproteins change during infection remains unclear. Here using conformation-specific monoclonal antibodies we show in situ that each component of the Murid Herpesvirus-4 (MuHV-4 entry machinery--gB, gH/gL and gp150--changes in antigenicity before tegument protein release begins. Further changes then occurred upon actual membrane fusion. Thus virions revealed their final fusogenic form only in late endosomes. The substantial antigenic differences between this form and that of extracellular virions suggested that antibodies have only a limited opportunity to block virion membrane fusion.

Three-Dimensionally Functionalized Reverse Phase Glycoprotein Array for Cancer Biomarker Discovery and Validation.

Science.gov (United States)

Pan, Li; Aguilar, Hillary Andaluz; Wang, Linna; Iliuk, Anton; Tao, W Andy

2016-11-30

Glycoproteins have vast structural diversity that plays an important role in many biological processes and have great potential as disease biomarkers. Here, we report a novel functionalized reverse phase protein array (RPPA), termed polymer-based reverse phase glycoprotein array (polyGPA), to capture and profile glycoproteomes specifically, and validate glycoproteins. Nitrocellulose membrane functionalized with globular hydroxyaminodendrimers was used to covalently capture preoxidized glycans on glycoproteins from complex protein samples such as biofluids. The captured glycoproteins were subsequently detected using the same validated antibodies as in RPPA. We demonstrated the outstanding specificity, sensitivity, and quantitative capabilities of polyGPA by capturing and detecting purified as well as endogenous α-1-acid glycoprotein (AGP) in human plasma. We further applied quantitative N-glycoproteomics and the strategy to validate a panel of glycoproteins identified as potential biomarkers for bladder cancer by analyzing urine glycoproteins from bladder cancer patients or matched healthy individuals.

Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages.

Science.gov (United States)

Zhu, R; Zacharias, L; Wooding, K M; Peng, W; Mechref, Y

2017-01-01

Protein glycosylation is one of the most important posttranslational modifications. Numerous biological functions are related to protein glycosylation. However, analytical challenges remain in the glycoprotein analysis. To overcome the challenges associated with glycoprotein analysis, many analytical techniques were developed in recent years. Enrichment methods were used to improve the sensitivity of detection, while HPLC and mass spectrometry methods were developed to facilitate the separation of glycopeptides/proteins and enhance detection, respectively. Fragmentation techniques applied in modern mass spectrometers allow the structural interpretation of glycopeptides/proteins, while automated software tools started replacing manual processing to improve the reliability and throughput of the analysis. In this chapter, the current methodologies of glycoprotein analysis were discussed. Multiple analytical techniques are compared, and advantages and disadvantages of each technique are highlighted. © 2017 Elsevier Inc. All rights reserved.

Iron Fertilization of the Southern Ocean: Regional Simulation and Analysis of C-Sequestration in the Ross Sea

Energy Technology Data Exchange (ETDEWEB)

Kevin Arrigo

2012-03-13

A modified version of the dynamic 3-dimensional mesoscale Coupled Ice, Atmosphere, and Ocean model (CIAO) of the Ross Sea ecosystem has been used to simulate the impact of environmental perturbations upon primary production and biogenic CO2 uptake. The Ross Sea supports two taxonomically, and spatially distinct phytoplankton populations; the haptophyte Phaeocystis antarctica and diatoms. Nutrient utilization ratios predict that P. antarctica and diatoms will be driven to nitrate and phosphate limitation, respectively. Model and field data have confirmed that the Ross Sea is iron limited with only two-thirds of the macronutrients consumed by the phytoplankton by the end of the growing season. In this study, the CIAO model was improved to simulate a third macronutrient (phosphate), dissolved organic carbon, air-sea gas exchange, and the carbonate system. This enabled us to effectively model pCO2 and subsequently oceanic CO2 uptake via gas exchange, allowing investigations into the affect of alleviating iron limitation on both pCO2 and nutrient drawdown.

Ross Terrill, The New Chinese Empire — and what it means for the United States

OpenAIRE

Eyraud, Henri

2007-01-01

Ross Terrill part du constat que la Chine est restée, jusqu'à maintenant, un empire sans contre-pouvoirs et répressif. Mao Zedong, Deng Xiaoping, Jiang Zemin et aujourd'hui Hu Jintao, chacun à leur manière et à leur époque, ont adapté, voire modernisé, les formes de pouvoir de l'empire millénaire. Pour l'auteur, la République populaire de Chine (RPC) a conservé une conception impériale d'elle-même et a réinventé l'autocratie millénaire. Ross Terrill est un observateur politique, mais aussi u...

Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production

International Nuclear Information System (INIS)

Jarvis, Donald L.

2003-01-01

The baculovirus-insect cell expression system is widely used to produce recombinant glycoproteins for many different biomedical applications. However, due to the fundamental nature of insect glycoprotein processing pathways, this system is typically unable to produce recombinant mammalian glycoproteins with authentic oligosaccharide side chains. This minireview summarizes our current understanding of insect protein glycosylation pathways and our recent efforts to address this problem. These efforts have yielded new insect cell lines and baculoviral vectors that can produce recombinant glycoproteins with humanized oligosaccharide side chains

Report of the Dutch experience with the Ross procedure in 343 patients

NARCIS (Netherlands)

Takkenberg, J. J. M.; Dossche, K. M. E.; Hazekamp, M. G.; Nijveld, A.; Jansen, E. W. L.; Waterbolk, T. W.; Bogers, A. J. J. C.

2002-01-01

Limited information is available on outcome after autograft aortic valve replacement, in particular with respect to the durability of the autograft and of the allograft used to reconstruct the right ventricular outflow tract. A retrospective follow-up study of all patients who underwent a Ross

76 FR 22338 - Proposed Fort Ross-Seaview Viticultural Area; Comment Period Reopening

Science.gov (United States)

2011-04-21

... May 9, 2005, from all interested persons. In response to a request from a local wine industry member... the Fort Ross-Seaview viticultural area. Two local wine industry members supported the petition without qualification; a third industry member supported the viticultural area's establishment while...

Duality of Ross Ice Shelf systems: crustal boundary, ice sheet processes and ocean circulation from ROSETTA-Ice surveys

Science.gov (United States)

Tinto, K. J.; Siddoway, C. S.; Padman, L.; Fricker, H. A.; Das, I.; Porter, D. F.; Springer, S. R.; Siegfried, M. R.; Caratori Tontini, F.; Bell, R. E.

2017-12-01

Bathymetry beneath Antarctic ice shelves controls sub-ice-shelf ocean circulation and has a major influence on the stability and dynamics of the ice sheets. Beneath the Ross Ice Shelf, the sea-floor bathymetry is a product of both tectonics and glacial processes, and is influenced by the processes it controls. New aerogeophysical surveys have revealed a fundamental crustal boundary bisecting the Ross Ice Shelf and imparting a duality to the Ross Ice Shelf systems, encompassing bathymetry, ocean circulation and ice flow history. The ROSETTA-Ice surveys were designed to increase the resolution of Ross Ice Shelf mapping from the 55 km RIGGS survey of the 1970s to a 10 km survey grid, flown over three years from New York Air National Guard LC130s. Radar, LiDAR, gravity and magnetic instruments provide a top to bottom profile of the ice shelf and the underlying seafloor, with 20 km resolution achieved in the first two survey seasons (2015 and 2016). ALAMO ocean-profiling floats deployed in the 2016 season are measuring the temperature and salinity of water entering and exiting the sub-ice water cavity. A significant east-west contrast in the character of the magnetic and gravity fields reveals that the lithospheric boundary between East and West Antarctica exists not at the base of the Transantarctic Mountains (TAM), as previously thought, but 300 km further east. The newly-identified boundary spatially coincides with the southward extension of the Central High, a rib of shallow basement identified in the Ross Sea. The East Antarctic side is characterized by lower amplitude magnetic anomalies and denser TAM-type lithosphere compared to the West Antarctic side. The crustal structure imparts a fundamental duality on the overlying ice and ocean, with deeper bathymetry and thinner ice on the East Antarctic side creating a larger sub-ice cavity for ocean circulation. The West Antarctic side has a shallower seabed, more restricted ocean access and a more complex history of

Regulation of glycoprotein synthesis in yeast by mating pheromones

International Nuclear Information System (INIS)

Tanner, W.

1984-01-01

In Saccharomyces cerevisiae, glycosylated proteins amount to less than 2% of the cell protein. Two intensively studied examples of yeast glycoproteins are the external cell wall - associated invertase and the vacuolar carboxypeptidase Y. Recently, it was shown that the mating pheromone, alpha factor, specifically and strongly inhibits the synthesis of N-glycosylated proteins in haploid a cells, whereas O-glycosylated proteins are not affected. In this paper, the pathways of glycoprotein biosynthesis are summarized briefly, and evidence is presented that mating pheomones have a regulatory function in glycoprotein synthesis

Engineered CHO cells for production of diverse, homogeneous glycoproteins

DEFF Research Database (Denmark)

Yang, Zhang; Wang, Shengjun; Halim, Adnan

2015-01-01

Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferas...

The place of theology in the modern university | Ross | Journal of ...

African Journals Online (AJOL)

The place of theology in the modern university. Kenneth R. Ross. Abstract. No Abstract. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about ...

Astronauts Ross and Helms at CAPCOM station during STS-61 simulations

Science.gov (United States)

1993-01-01

Astronauts Jerry L. Ross and Susan J. Helms are pictured at the Spacecraft Communicators console during joint integrated simulations for the STS-61 mission. Astronauts assigned to extravehicular activity (EVA) tasks with the Hubble Space Telescope (HST) were simultaneously rehearsing in a neutral buoyancy tank at the Marshall Space Flight Center (MSFC) in Alabama.

Bioinformatics Analysis of Envelope Glycoprotein E epitopes of ...

African Journals Online (AJOL)

The E glycoprotein of dengue virus is responsible for the viral binding to the receptor. The crystal structure of envelope glycoprotein has already been determined. However, where the well-defined Bcell and T-cell epitopes are located is still a question. Because of the large variations among the four dengue genotypes, it is ...

The Purification of a Blood Group A Glycoprotein: An Affinity Chromatography Experiment.

Science.gov (United States)

Estelrich, J.; Pouplana, R.

1988-01-01

Describes a purification process through affinity chromatography necessary to obtain specific blood group glycoproteins from erythrocytic membranes. Discusses the preparation of erythrocytic membranes, extraction of glycoprotein from membranes, affinity chromatography purification, determination of glycoproteins, and results. (CW)

STS-55 MS1/PLC Ross and Payload Specialist Walter work in SL-D2 module

Science.gov (United States)

1993-01-01

STS-55 Mission Specialist 1 (MS1) and Payload Commander (PLC) Jerry L. Ross floats near cycle ergometer and Rack 9 Anthrorack (AR) (Human Physiology Laboratory) as German Payload Specialist 1 Ulrich Walter reviews a checklist in front of Rack 11 Experiment Rack. These experiment stations and the crewmembers are in the shirt-sleeve environment of the Spacelab Deutsche 2 (SL-D2) science module onboard the Earth-orbiting Columbia, Orbiter Vehicle (OV) 102. In the background is the SL-D2 aft end cone. Behind Ross and Walter is Rack 12 Experiment Rack with Baroreflex (BA).

Visioni vittoriane: il paesaggio fiorentino nelle opere di Janet Ross e Vernon Lee

Directory of Open Access Journals (Sweden)

Gabriele Corsani

2015-11-01

Full Text Available Fra l’ultimo scorcio dell’Ottocento e i primi decenni del Novecento Firenze e i suoi dintorni sono il soggetto privilegiato di una grande quantità di descrizioni, note di diario, racconti, opera di scrittori stranieri, in particolare inglesi, che si radicano nell’approdo elettivo di quei luoghi. Il testo presenta la traccia letteraria del paesaggio fiorentino nelle opere di Vernon Lee e Janet Ross, due tipiche rappresentanti di questa tendenza che hanno vissuto, attraverso vicende biografiche in qualche modo parallele, un’esperienza di intensa identificazione con il paesaggio fiorentino. Di Janet Ross, viene commentato Old Florence and Modern Tuscany, volume che raccoglie una serie di articoli pubblicati su alcune riviste inglesi e fornisce una efficace panoramica sull’interesse molto concreto di Janet Ross per il mondo rurale che la vede addirittura impegnata nella gestione della fattoria di Castagnolo, a Lastra a Signa. Più sfaccettato è il commento alle opere di Vernon Lee, di cui sono commentati passi da Vanitas. Polite Stories, Genius Loci, Hortus Vitae and Limbo, in virtù della maggiore ampiezza e complessità del suo mondo culturale. Ne sono cifra distintiva la associazione fra storia e realtà attraverso la dimensione del mistero, che risulta una delle chiavi di acccesso alla bellezza e alla vitalità del paesagggio e la capacità di cogliere il ritmo proprio dei luoghi e di entrare in reale sintonia con essi. 

Geometric controls of the flexural gravity waves on the Ross Ice Shelf

Science.gov (United States)

Sergienko, O. V.

2017-12-01

Long-period ocean waves, formed locally or at distant sources, can reach sub-ice-shelf cavities and excite coupled motion in the cavity and the ice shelf - flexural gravity waves. Three-dimensional numerical simulations of the flexural gravity waves on the Ross Ice Shelf show that propagation of these waves is strongly controlled by the geometry of the system - the cavity shape, its water-column thickness and the ice-shelf thickness. The results of numerical simulations demonstrate that propagation of the waves is spatially organized in beams, whose orientation is determined by the direction of the of the open ocean waves incident on the ice-shelf front. As a result, depending on the beams orientation, parts of the Ross Ice Shelf experience significantly larger flexural stresses compared to other parts where the flexural gravity beams do not propagate. Very long-period waves can propagate farther away from the ice-shelf front exciting flexural stresses in the vicinity of the grounding line.

Tidal Modulation of Ice-shelf Flow: a Viscous Model of the Ross Ice Shelf

Science.gov (United States)

Brunt, Kelly M.; MacAyeal, Douglas R.

2014-01-01

Three stations near the calving front of the Ross Ice Shelf, Antarctica, recorded GPS data through a full spring-neap tidal cycle in November 2005. The data revealed a diurnal horizontal motion that varied both along and transverse to the long-term average velocity direction, similar to tidal signals observed in other ice shelves and ice streams. Based on its periodicity, it was hypothesized that the signal represents a flow response of the Ross Ice Shelf to the diurnal tides of the Ross Sea. To assess the influence of the tide on the ice-shelf motion, two hypotheses were developed. The first addressed the direct response of the ice shelf to tidal forcing, such as forces due to sea-surface slopes or forces due to sub-ice-shelf currents. The second involved the indirect response of ice-shelf flow to the tidal signals observed in the ice streams that source the ice shelf. A finite-element model, based on viscous creep flow, was developed to test these hypotheses, but succeeded only in falsifying both hypotheses, i.e. showing that direct tidal effects produce too small a response, and indirect tidal effects produce a response that is not smooth in time. This nullification suggests that a combination of viscous and elastic deformation is required to explain the observations.

Pulmonary homograft stenosis in the Ross procedure: Incidence, clinical impact and predictors in long-term follow-up.

Science.gov (United States)

Pardo González, Laura; Ruiz Ortiz, Martin; Delgado, Mónica; Mesa, Dolores; Villalba, Rafael; Rodriguez, Sara; Hidalgo, Francisco J; Alados, Pedro; Casares, Jaime; Suarez de Lezo, Jose

2017-04-01

The Ross procedure is used in the treatment of selected patients with aortic valve disease. Pulmonary graft stenosis can appear in the long-term follow-up after the Ross intervention, but the factors involved and its clinical implications are not fully known. To describe the incidence, clinical impact and predictors of homograft stenosis and reintervention after the Ross procedure in a prospective series in a tertiary referral hospital. From 1997 to 2009, 107 patients underwent the Ross procedure (mean age: 30±11 years; 69% men; 21 aged36mmHg) and surgical or percutaneous homograft reintervention. After 15 years of follow-up (median: 11 years), echocardiographic and clinical data were available in 91 (85%) and 104 (98%) patients, respectively: 26/91 (29%) patients developed homograft stenosis; 10/104 (10%) patients underwent 13 homograft reintervention procedures (three patients underwent surgical replacement, three received a percutaneous pulmonary valve and one needed stent implantation). The other three patients underwent two consecutive procedures in follow-up; one died because of a procedure-related myocardial infarction. Rates of survival free from homograft stenosis and reintervention at 1, 5 and 10 years were 96%, 82% and 75% and 99%, 94% and 91%, respectively. Paediatric patients had worse survival free from homograft stenosis (hazard ratio [HR] 3.50, 95% confidence interval [CI]: 1.56-7.90; P=0.002), although there were no significant differences regarding reintervention (HR: 2.01, 95% CI: 0.52-7.78; P=0.31). Younger age of homograft donor was also a stenosis predictor (HR: 0.97, 95% CI: 0.94-0.99; P=0.046). The probabilities of homograft stenosis and reintervention 10 years after the Ross procedure were 29% and 10%, respectively; only one patient had a reintervention-related death. Younger donor and recipient age were associated with a higher rate of stenosis. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Elisabeth Kubler-Ross and the Tradition of the Private Sphere: An Analysis of Symbols.

Science.gov (United States)

Klass, Dennis

1981-01-01

Shows how Kubler-Ross' schema functions as a symbol system. Analyzes the symbol "acceptance." Shows how that symbol is part of a strong American tradition of symbols of the private sphere. (Author/JAC)

Multichannel Seismic Reflection Data - SCAR - Ross Sea 1987-1988, SDLS CD-ROM vol 2

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These data are stacked multichannel marine seismic reflection data from six lines recorded during 1988 in the Ross Sea, Antarctica, by the Bundesanstalt fur...

New data on Weddell seal (Leptonychotes weddellii colonies: A genetic analysis of a top predator from the Ross Sea, Antarctica.

Directory of Open Access Journals (Sweden)

Ighor Antunes Zappes

Full Text Available In this paper, we studied the genetic variability in Weddell seal from colonies in Terra Nova Bay and Wood Bay, both sites located in the Ross Sea area, Antarctica. Two mitochondrial genes and one nuclear gene, with different mutation rates, were sequenced to investigate the haplotype diversity of the colonies and to test for a possible recent expansion. Fifteen microsatellites were used to analyze their genetic structure. Sequenced genes and microsatellites were also used to estimate the effective population size of the studied colonies and the Ross Sea seal population. The Ross Sea has a high density population of Weddel seals, with an estimated effective number of 50,000 females, and 1,341 individuals for the sampling area, possibly due to its high primary production. The colonies showed high diversity (Hd > 0.90 and many exclusive haplotypes (> 75%, likely a consequence of the surprisingly high site fidelity of Weddell seals, despite the proximity of the colonies. Nevertheless, there was low microsatellite differentiation between colonies, suggesting that they are part of a single larger population. Their expansion seemed to have started during the last glacial cycle (around 58,000 years ago, indicating that the Ross Sea seal populations have been present in the area for long time, probably due to the lack of hunting by humans and terrestrial predation. As a top predator, the role of Weddell seals in the Ross Sea ecology is crucial, and its demographic dynamics should be monitored to follow the future changes of such an important ecosystem.

Isolation and characterization of two antigenic glycoproteins from the pollen of Prosopis juliflora.

Science.gov (United States)

Thakur, I S

1991-03-01

Two antigenically active glycoprotein fractions were isolated from crude extract of the pollen of Prosopis juliflora using DEAE-cellulose ion exchange chromatography. The glycoproteins gave single band on polyacrylamide gel electrophoresis. The molecular weight of these two glycoprotein was 20,000 and 10,000 as determined by gel filtration on Sephadex G-75. With the help of crossed immunoelectrophoresis and gel diffusion crude extract exhibited twelve and three precipitating antigens suggesting its heterogeneous nature; and the purified glycoprotein fractions however formed single precipitin band on gel diffusion test and immunoelectrophoresis. As tested by ELISA the polyclonal antisera raised in rabbit showed strong binding affinity with glycoprotein of MW 20,000. These result indicates that the two glycoprotein fractions are not antigenically identical.

The influence of the Amundsen Sea Low on the winds in the Ross Sea and surroundings: Insights from a synoptic climatology

Science.gov (United States)

Coggins, Jack H. J.; McDonald, Adrian J.

2015-03-01

The Amundsen Sea Low (ASL) is an area of climatologically low atmospheric pressure situated over the Southern Ocean. The depth and location of this feature have significant effects on winds, temperature, moisture transport, and sea ice in its vicinity. In this article, we quantify the modulating effect of this feature on winds over the Ross Sea and Ross Ice Shelf. We examine composites of surface winds sampled according to extrema in ASL depth, longitude, and latitude. We employ the output of a previously developed synoptic climatology to identify the explanatory synoptic-scale forcings. In autumn, winter, and spring (AWS) we find that the impact of the depth of the ASL is smaller than that of its location. The ASL moves eastward when it is deep, thereby reducing its influence on Ross Sea winds. When the ASL is northward, we find strongly enhanced southerly flows over the Ross Sea and Ice Shelf, forced by greater cyclonic activity in the north of the Ross Sea. In summer, we find increased cyclonic flow coinciding with a deeper ASL, despite the ASL being located in the Bellingshausen Sea at this time. The responses to the ASL longitude and latitude are profoundly different to those in AWS, suggesting that relationships are strongly dependent on the varying seasonal location of the low. We examine two metrics of the ASL depth and identify that the absolute mean sea level pressure (MSLP) has a more widespread response than that of the relative MSLP.

Oceanographic mechanisms and penguin population increases during the Little Ice Age in the southern Ross Sea, Antarctica

Science.gov (United States)

Yang, Lianjiao; Sun, Liguang; Emslie, Steven D.; Xie, Zhouqing; Huang, Tao; Gao, Yuesong; Yang, Wenqing; Chu, Zhuding; Wang, Yuhong

2018-01-01

The Adélie penguin is a well-known indicator for climate and environmental changes. Exploring how large-scale climate variability affects penguin ecology in the past is essential for understanding the responses of Southern Ocean ecosystems to future global change. Using ornithogenic sediments at Cape Bird, Ross Island, Antarctica, we inferred relative population changes of Adélie penguins in the southern Ross Sea over the past 500 yr, and observed an increase in penguin populations during the Little Ice Age (LIA; 1500-1850 AD). We used cadmium content in ancient penguin guano as a proxy of ocean upwelling and identified a close linkage between penguin dynamics and atmospheric circulation and oceanic conditions. During the cold period of ∼1600-1825 AD, a deepened Amundsen Sea Low (ASL) led to stronger winds, intensified ocean upwelling, enlarged Ross Sea and McMurdo Sound polynyas, and thus higher food abundance and penguin populations. We propose a mechanism linking Antarctic marine ecology and atmospheric/oceanic dynamics which can help explain and predict responses of Antarctic high latitudes ecosystems to climate change.

Wandering gravel-bed rivers and high-constructive stable channel sandy fluvial systems in the Ross River area, Yukon Territory, Canada

Directory of Open Access Journals (Sweden)

Darrel G.F. Long

2011-07-01

Gravel-dominated strata, inter-bedded with, and overlying coal-bearing units, are interpreted as deposits of wandering gravel-bed rivers, with sinuosity approaching 1.4. In most exposures they appear to be dominated by massive and thin planar-bedded granule to small pebble conglomerates, which would traditionally be interpreted as sheet-flood or longitudinal bar deposits of a high-gradient braided stream or alluvial fan. Architectural analysis of exposures in an open-pit shows that the predominance of flat bedding is an artefact of the geometry of the roadside exposures. In the pit the conglomerates are dominated by large scale cross stratification on a scale of 1–5.5 m. These appear to have developed as downstream and lateral accretion elements on side-bars and on in-channel bars in water depths of 2–12 m. Stacking of strata on domed 3rd order surfaces suggests development of longitudinal in-channel bar complexes similar to those observed in parts of the modern Rhône River system. Mudstone preserved in some of the channels reflects intervals of channel abandonment or avulsion. Minimum channel width is from 70 to 450 m.

Purification and characterization of the glycoprotein allergen from Prosopis juliflora pollen.

Science.gov (United States)

Thakur, I S

1991-02-01

Highly active glycoprotein allergens have been isolated from pollen of Prosopis juliflora by a combination of Sephadex G-100 gel filtration and Sodium dodecyl sulphate-Poly-acrylamide gel electrophoresis. The glycoprotein fraction was homogeneous, and had molecular weight 20,000. The purified glycoprotein allergen contained 20% carbohydrate, mainly arabinose and galactose. Enzymatic digestion of glycoprotein with protease released glycopeptides of molecular weight ranging from less than 1,000 to more than 5,000 on Sephadex G-25 gel filtration. Antigenicity or allergenicity testing of these glycopeptides by immunodiffusion, immunoelectrophoresis, and radioallergosorbent test indicated complete loss of allergenic activity after digestion with protease whereas incubation with beta-D-galactosidase and periodate oxidation had little affect on the allergenic activity of the glycoprotein fraction. But incubation with alpha-D-glucosidase did not affect the allergenic activity significantly. All these tests indicated that protein played significant role in allergenicity of P. juliflora pollen.

Strategies to overcome or circumvent P-glycoprotein mediated multidrug resistance.

Science.gov (United States)

Yuan, Hongyu; Li, Xun; Wu, Jifeng; Li, Jinpei; Qu, Xianjun; Xu, Wenfang; Tang, Wei

2008-01-01

Cancer patients who receive chemotherapy often experience intrinsic or acquired resistance to a broad spectrum of chemotherapeutic agents. The phenomenon, termed multidrug resistance (MDR), is often associated with the over-expression of P-glycoprotein, a transmembrane protein pump, which can enhance efflux of a various chemicals structurally unrelated at the expense of ATP depletion, resulting in decrease of the intracellular cytotoxic drug accumulation. The MDR has been a big threaten to the human health and the war fight for it continues. Although several other mechanisms for MDR are elucidated in recent years, considerable efforts attempting to inverse MDR are involved in exploring P-glycoprotein modulators and suppressing P-glycoprotein expression. In this review, we will report on the recent advances in various strategies for overcoming or circumventing MDR mediated by P-glycoprotein.

76 FR 81962 - Final Environmental Impact Statement for General Management Plan, Ross Lake National Recreation...

Science.gov (United States)

2011-12-29

... soundscapes, and scenery through traditional outdoor activities. The NPS would actively manage to reduce... Ross Lake NRA in order to protect and enhance soundscapes and wilderness character, experience, and...

A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection.

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Takuya Kanno

Full Text Available Therapeutic agents to the central nervous system (CNS need to be efficiently delivered to the target site of action at appropriate therapeutic levels. However, a limited number of effective drugs for the treatment of neurological diseases has been developed thus far. Further, the pharmacological mechanisms by which such therapeutic agents can protect neurons from cell death have not been fully understood. We have previously reported the novel small-molecule compound, 2-[mesityl(methylamino]-N-[4-(pyridin-2-yl-1H-imidazol-2-yl] acetamide trihydrochloride (WN1316, as a unique neuroprotectant against oxidative injury and a highly promising remedy for the treatment of amyotrophic lateral sclerosis (ALS. One of the remarkable characteristics of WN1316 is that its efficacious doses in ALS mouse models are much less than those against oxidative injury in cultured human neuronal cells. It is also noted that the WN1316 cytoprotective activity observed in cultured cells is totally dependent upon the addition of fetal bovine serum in culture medium. These findings led us to postulate some serum factors being tightly linked to the WN1316 efficacy. In this study, we sieved through fetal bovine serum proteins and identified two N-linked glycoproteins, alpha-2-HS-glycoprotein (AHSG and hemopexin (HPX, requisites to exert the WN1316 cytoprotective activity against oxidative injury in neuronal cells in vitro. Notably, the removal of glycan chains from these molecules did not affect the WN1316 cytoprotective activity. Thus, two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy.

Trends in the breeding population of Adelie penguins in the Ross Sea, 1981-2012: a coincidence of climate and resource extraction effects.

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Phil O'B Lyver

Full Text Available Measurements of the size of Adélie penguin (Pygoscelis adeliae colonies of the southern Ross Sea are among the longest biologic time series in the Antarctic. We present an assessment of recent annual variation and trends in abundance and growth rates of these colonies, adding to the published record not updated for more than two decades. High angle oblique aerial photographic surveys of colonies were acquired and penguins counted for the breeding seasons 1981-2012. In the last four years the numbers of Adélie penguins in the Ross and Beaufort Island colonies (southern Ross Sea metapopulation reached their highest levels since aerial counts began in 1981. Results indicated that 855,625 pairs of Adélie penguins established breeding territories in the western Ross Sea, with just over a quarter (28% of those in the southern portion, constituting a semi-isolated metapopulation (three colonies on Ross Island, one on nearby Beaufort Island. The southern population had a negative per capita growth rate of -0.019 during 1981-2000, followed by a positive per capita growth rate of 0.067 for 2001-2012. Colony growth rates for this metapopulation showed striking synchrony through time, indicating that large-scale factors influenced their annual growth. In contrast to the increased colony sizes in the southern population, the patterns of change among colonies of the northern Ross Sea were difficult to characterize. Trends were similar to southern colonies until the mid-1990s, after which the signal was lost owing to significantly reduced frequency of surveys. Both climate factors and recovery of whale populations likely played roles in the trends among southern colonies until 2000, after which depletion of another trophic competitor, the Antarctic toothfish (Dissostichus mawsoni, may explain the sharp increasing trend evident since then.

Labelled antibody techniques in glycoprotein estimation

International Nuclear Information System (INIS)

Hazra, D.K.; Ekins, R.P.; Edwards, R.; Williams, E.S.

1977-01-01

The problems in the radioimmunoassay of the glycoprotein hormones (pituitary LH, FSH and TSH and human chlorionic gonadotrophin HGG) are reviewed viz: limited specificity and sensitivity in the clinical context, interpretation of disparity between bioassay and radioimmunoassay, and interlaboratory variability. The advantages and limitations of the labelled antibody techniques - classical immonoradiometric methods and 2-site or 125 I-anti-IgG indirect labelling modifications are reviewed in general, and their theoretical potential in glycoprotein assays examined in the light of previous work. Preliminary experiments in the development of coated tube 2-site assay for glycoproteins using 125 I anti-IgG labelling are described, including conditions for maximizing solid phase extraction of the antigen, iodination of anti-IgG, and assay conditions such as effects of temperature of incubation with antigen 'hormonefree serum', heterologous serum and detergent washing. Experiments with extraction and antigen-specific antisera raised in the same or different species are described as exemplified by LH and TSH assay systems, the latter apparently promising greater sensitivity than radioimmunoassay. Proposed experimental and mathematical optimisation and validation of the method as an assay system is outlined, and the areas for further work delineated. (orig.) [de

Structures and Functions of Pestivirus Glycoproteins: Not Simply Surface Matters.

Science.gov (United States)

Wang, Fun-In; Deng, Ming-Chung; Huang, Yu-Liang; Chang, Chia-Yi

2015-06-29

Pestiviruses, which include economically important animal pathogens such as bovine viral diarrhea virus and classical swine fever virus, possess three envelope glycoproteins, namely Erns, E1, and E2. This article discusses the structures and functions of these glycoproteins and their effects on viral pathogenicity in cells in culture and in animal hosts. E2 is the most important structural protein as it interacts with cell surface receptors that determine cell tropism and induces neutralizing antibody and cytotoxic T-lymphocyte responses. All three glycoproteins are involved in virus attachment and entry into target cells. E1-E2 heterodimers are essential for viral entry and infectivity. Erns is unique because it possesses intrinsic ribonuclease (RNase) activity that can inhibit the production of type I interferons and assist in the development of persistent infections. These glycoproteins are localized to the virion surface; however, variations in amino acids and antigenic structures, disulfide bond formation, glycosylation, and RNase activity can ultimately affect the virulence of pestiviruses in animals. Along with mutations that are driven by selection pressure, antigenic differences in glycoproteins influence the efficacy of vaccines and determine the appropriateness of the vaccines that are currently being used in the field.

Characterization of pH-sensitive molecular switches that trigger the structural transition of vesicular stomatitis virus glycoprotein from the postfusion state toward the prefusion state.

Science.gov (United States)

Ferlin, Anna; Raux, Hélène; Baquero, Eduard; Lepault, Jean; Gaudin, Yves

2014-11-01

Vesicular stomatitis virus (VSV; the prototype rhabdovirus) fusion is triggered at low pH and mediated by glycoprotein G, which undergoes a low-pH-induced structural transition. A unique feature of rhabdovirus G is that its conformational change is reversible. This allows G to recover its native prefusion state at the viral surface after its transport through the acidic Golgi compartments. The crystal structures of G pre- and postfusion states have been elucidated, leading to the identification of several acidic amino acid residues, clustered in the postfusion trimer, as potential pH-sensitive switches controlling the transition back toward the prefusion state. We mutated these residues and produced a panel of single and double mutants whose fusion properties, conformational change characteristics, and ability to pseudotype a virus lacking the glycoprotein gene were assayed. Some of these mutations were also introduced in the genome of recombinant viruses which were further characterized. We show that D268, located in the segment consisting of residues 264 to 273, which refolds into postfusion helix F during G structural transition, is the major pH sensor while D274, D395, and D393 have additional contributions. Furthermore, a single passage of recombinant virus bearing the mutation D268L (which was demonstrated to stabilize the G postfusion state) resulted in a pseudorevertant with a compensatory second mutation, L271P. This revealed that the propensity of the segment of residues 264 to 273 to refold into helix F has to be finely tuned since either an increase (mutation D268L alone) or a decrease (mutation L271P alone) of this propensity is detrimental to the virus. Vesicular stomatitis virus enters cells via endocytosis. Endosome acidification induces a structural transition of its unique glycoprotein (G), which mediates fusion between viral and endosomal membranes. G conformational change is reversible upon increases in pH. This allows G to recover its native

Experiência cirúrgica inicial com a operação de Ross (auto-enxerto pulmonar Initial surgical experience with the Ross procedure (pulmonary autograft

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Francisco Diniz Affonso da Costa

1996-06-01

Full Text Available FUNDAMENTO: Em decorrência dos excelentes resultados tardios observados com a operação de Ross, sua utilização tem sido cada vez mais freqüente em vários Centros de todo o mundo. OBJETIVO: Relatar a experiência cirúrgica inicial com essa operação em nosso meio. CASUÍSTICA E MÉTODOS: De maio/95 a fevereiro/96,24 pacientes com média de idades de 28,3 anos foram submetidos à operação de Ross pelo método de substituição do segmento proximal da aorta ascendente. Para a reconstrução da via de saída do ventrículo direito, foram utilizados 17 homoenxertos pulmonares e 7 homoenxertos aórticos preservados em solução de antibióticos. Em todos os pacientes foram realizados ecocardiograma bidimensional com Doppler e cateterismo cardíaco no pós-operatório imediato, para avaliar a função ventricular e desempenho hemodinâmico dos auto e homoenxertos utilizados. Três pacientes com evolução clínica superior a seis meses realizaram novo ecocardiograma. RESULTADOS: A mortalidade hospitalar foi de 4%. Os sobreviventes tiveram alta hospitalar em ritmo sinusal e sem sopro diastólico de insuficiência aórtica. O desempenho hemodinâmico dos auto-enxertos foi muito satisfatório, com baixos gradientes de pico (4,0 ± 1,3 mmHg pelo ecocardiograma e 2,8 ± 1,2 mmHg pelo cateterismo. Vinte e um pacientes apresentaram auto-enxertos suficientes e/ou com insuficiência trivial, e 2 pacientes tiveram insuficiência leve. Nenhum paciente teve insuficiência moderada ou importante. Os gradientes de pico dos homoenxertos também foram baixos (3,0 ± 0,9 mmHg pelo ecocardiograma e 4,3 ± 1,4 mmHg pelo cateterismo e apenas 2 apresentaram insuficiência leve. Houve significativa redução das dimensões sistólica e diastólica do ventrículo esquerdo no pós-operatório imediato, assim como da massa ventricular esquerda. Após um tempo médio de seguimento clínico de 5,1 meses (1-9 meses, todos os pacientes encontram-se em classe funcional I

STS-37 Mission Specialist (MS) Ross during simulation in JSC's FB-SMS

Science.gov (United States)

1991-01-01

STS-37 Mission Specialist (MS) Jerry L. Ross 'borrows' the pilots station to rehearse some of his scheduled duties for his upcoming mission. He is on the flight deck of the fixed-based (FB) shuttle mission simulator (SMS) during this unsuited simulation. The SMS is part of JSC's Mission Simulation and Training Facility Bldg 5.

Aortic valve repair for insufficiency in older children offers unpredictable durability that may not be advantageous over a primary Ross operation†.

Science.gov (United States)

Wilder, Travis J; Caldarone, Christopher A; Van Arsdell, Glen S; Pham-Hung, Eric; Gritti, Michael; Al Jughiman, Mohammed; Hickey, Edward J

2016-03-01

To evaluate the durability of aortic valve (AoV) repair relative to other strategies for children with significant aortic insufficiency (AI). From 2001 to 2012, 90 children with greater than or equal to moderate AI underwent surgery. Resulting procedures were classified according to final operative outcome: AoV repair (repair; n = 46, 51%), Ross procedure (Ross; n = 21, 23%) or replacement with mechanical or tissue prosthesis [aortic valve replacement (AVR); n = 23, 26%]. Repeated measures (n = 1081 echocardiograms) mixed-model analysis and parametric multiphase risk-adjusted hazard analysis were used to evaluate haemodynamic parameters and durability of operations. Mean age at operation was similar for repair and Ross groups, but slightly higher for the AVR group (10.6, 11 and 13.2, respectively; P = 0.04). Baseline annular dimensions were similar among groups. Of 46 repairs, 85% involved pericardial leaflet extensions (commonly with leaflet shaving and/or commisuroplasty). The remaining repairs were commissuroplasties. On multivariable analysis, repair was associated with increased early (∼1-2 years) AI and increased outflow tract peak pressure gradients relative to Ross and AVR procedures. On univariate analysis, repairs tended to have a larger annulus size compared with Ross or AVR; however, this was not significant on multivariable analysis. There were 25 reinterventions (surgical reoperation = 16; transcatheter intervention = 9) for 22 children. Freedom from surgical reoperation was 64, 100 and 51% at 6 years for repairs, Ross and AVR, respectively (P = 0.05); however, three of five reoperations after AVR were for failed bioprosthetic devices. The freedom from reintervention was not significantly influenced by the type of AoV operation (P = 0.43). Durability of aortic valve repair for children is limited by recurrence of AI and/or stenosis, often within the first few years. After repair, reoperation should be anticipated within ∼7 years. © The Author

Adipokine zinc-α2-glycoprotein regulated by growth hormone and linked to insulin sensitivity.

Science.gov (United States)

Balaz, Miroslav; Ukropcova, Barbara; Kurdiova, Timea; Gajdosechova, Lucia; Vlcek, Miroslav; Janakova, Zuzana; Fedeles, Jozef; Pura, Mikulas; Gasperikova, Daniela; Smith, Steven R; Tkacova, Ruzena; Klimes, Iwar; Payer, Juraj; Wolfrum, Christian; Ukropec, Jozef

2015-02-01

Hypertrophic obesity is associated with impaired insulin sensitivity and lipid-mobilizing activity of zinc-α2-glycoprotein. Adipose tissue (AT) of growth hormone (GH) -deficient patients is characterized by extreme adipocyte hypertrophy due to defects in AT lipid metabolism. It was hypothesized that zinc-α2-glycoprotein is regulated by GH and mediates some of its beneficial effects in AT. AT from patients with GH deficiency and individuals with obesity-related GH deficit was obtained before and after 5-year and 24-month GH supplementation therapy. GH action was tested in primary human adipocytes. Relationships of GH and zinc-α2-glycoprotein with adipocyte size and insulin sensitivity were evaluated in nondiabetic patients with noncancerous cachexia and hypertrophic obesity. AT in GH-deficient adults displayed a substantial reduction of zinc-α2-glycoprotein. GH therapy normalized AT zinc-α2-glycoprotein. Obesity-related relative GH deficit was associated with almost 80% reduction of zinc-α2-glycoprotein mRNA in AT. GH increased zinc-α2-glycoprotein mRNA in both AT of obese men and primary human adipocytes. Interdependence of GH and zinc-α2-glycoprotein in regulating AT morphology and metabolic phenotype was evident from their relationship with adipocyte size and AT-specific and whole-body insulin sensitivity. The results demonstrate that GH is involved in regulation of AT zinc-α2-glycoprotein; however, the molecular mechanism linking GH and zinc-α2-glycoprotein in AT is yet unknown. © 2014 The Obesity Society.

Changes on the ice plain of Ice Stream B and Ross Ice Shelf

Science.gov (United States)

Shabtaie, Sion

1993-01-01

During the 1970's and 1980's, nearly 200 stations from which accurate, three dimensional position fixes have been obtained from TRANSIT satellites were occupied throughout the Ross Ice Shelf. We have transformed the elevations obtained by satellite altimetry to the same geodetic datum, and then applied a second transformation to reduce the geodetic heights to elevations above mean sea level using the GEM-10C geoidal height. On the IGY Ross Ice Shelf traverse between Oct. 1957 and Feb. 1958, an accurate method of barometric altimetry was used on a loop around the ice shelf that was directly tied to the sea at both ends of the travel route, thus providing absolute elevations. Comparisons of the two sets of data at 32 station pairs on floating ice show a mean difference of 0 +/- 1 m. The elevation data were also compared with theoretical values of elevations for a hydrostatically floating ice shelf. The mean difference between theoretical and measured values of elevations is -2 +/- 1 m.

Glycoprotein expression by adenomatous polyps of the colon

Science.gov (United States)

Roney, Celeste A.; Xie, Jianwu; Xu, Biying; Jabour, Paul; Griffiths, Gary; Summers, Ronald M.

2008-03-01

Colon cancer is the second leading cause of cancer related deaths in the United States. Specificity in diagnostic imaging for detecting colorectal adenomas, which have a propensity towards malignancy, is desired. Adenomatous polyp specimens of the colon were obtained from the mouse model of colorectal cancer called adenomatous polyposis coli-multiple intestinal neoplasia (APC Min). Histological evaluation, by the legume protein Ulex europaeus agglutinin I (UEA-1), determined expression of the glycoprotein α-L-fucose. FITC-labelled UEA-1 confirmed overexpression of the glycoprotein by the polyps on fluorescence microscopy in 17/17 cases, of which 13/17 included paraffin-fixed mouse polyp specimens. In addition, FITC-UEA-1 ex vivo multispectral optical imaging of 4/17 colonic specimens displayed over-expression of the glycoprotein by the polyps, as compared to non-neoplastic mucosa. Here, we report the surface expression of α-L-fucosyl terminal residues by neoplastic mucosal cells of APC specimens of the mouse. Glycoprotein expression was validated by the carbohydrate binding protein UEA-1. Future applications of this method are the development of agents used to diagnose cancers by biomedical imaging modalities, including computed tomographic colonography (CTC). UEA-1 targeting to colonic adenomas may provide a new avenue for the diagnosis of colorectal carcinoma by CT imaging.

Observing the seasonal cycle of the upper ocean in the Ross Sea, Antarctica, with autonomous profiling floats

Science.gov (United States)

Porter, D. F.; Springer, S. R.; Padman, L.; Fricker, H. A.; Bell, R. E.

2017-12-01

The upper layers of the Southern Ocean where it meets the Antarctic ice sheet undergoes a large seasonal cycle controlled by surface radiation and by freshwater fluxes, both of which are strongly influenced by sea ice. In regions where seasonal sea ice and icebergs limit use of ice-tethered profilers and conventional moorings, autonomous profiling floats can sample the upper ocean. The deployment of seven Apex floats (by sea) and six ALAMO floats (by air) provides unique upper ocean hydrographic data in the Ross Sea close to the Ross Ice Shelf front. A novel choice of mission parameters - setting parking depth deeper than the seabed - limits their drift, allowing us to deploy the floats close to the ice shelf front, while sea ice avoidance algorithms allow the floats to to sample through winter under sea ice. Hydrographic profiles show the detailed development of the seasonal mixed layer close to the Ross front, and interannual variability of the seasonal mixed layer and deeper water masses on the central Ross Sea continental shelf. After the sea ice breakup in spring, a warm and fresh surface mixed layer develops, further warming and deepening throughout the summer. The mixed layer deepens, with maximum temperatures exceeding 0ºC in mid-February. By March, the surface energy budget becomes negative and sea ice begins to form, creating a cold, saline and dense surface layer. Once these processes overcome the stable summer stratification, convection erodes the surface mixed layer, mixing some heat downwards to deeper layers. There is considerable interannual variability in the evolution and strength of the surface mixed layer: summers with shorter ice-free periods result in a cooler and shallower surface mixed layer, which accumulates less heat than the summers with longer ice-free periods. Early ice breakup occurred in all floats in 2016/17 summer, enhancing the absorbed solar flux leading to a warmer surface mixed layer. Together, these unique measurements from

Histochemical and structural analysis of mucous glycoprotein secreted by the gill of Mytilus edulis

International Nuclear Information System (INIS)

Ahn, Hae-Young.

1988-01-01

Studies were carried out to characterized various mucous cells in the gill filament, to ascertain structural characteristics of the secreted mucous glycoproteins, and to determine the ability of the gill epithelium to incorporate [ 14 C]glucosamine as a precursor in the biosynthesis and secretion of mucous glycoproteins. Using histochemical staining techniques, mucous cells containing neutral and acidic mucins were found in the lateral region, whereas mucous cells containing primarily neutral or sulfated mucins were found in the postlateral region. Serotonin, but not dopamine, stimulated the mucous secretion. In tissues pretreated with [ 14 C]glucosamine, the secreted glycoproteins contain incorporated radiolabel. Analysis by column chromatography using Bio-Gel P-2 and P-6 shows that the secretion contains two glycoprotein populations. Glycoprotein II has a molecular weight of 2.3 x 10 4 daltons. Upon alkaline reductive borohydride cleavage of the O-glycosidic linkages of glycoprotein I, about 70% of the radiolabel was removed from the protein. Gas chromatographic analysis of the carbohydrate composition shows that the glycoproteins contains N-acetylglucosamine (GluNAc), N-acetylgalactosamine (GalNAc), and galactose, fucose and mannose. Amino acid analysis shows that the glycoproteins are rich in serine, threonine and proline

Monoclonal antibody to an external epitope of the human mdr1 P-glycoprotein

NARCIS (Netherlands)

Arceci, R. J.; Stieglitz, K.; Bras, J.; Schinkel, A.; Baas, F.; Croop, J.

1993-01-01

A membrane glycoprotein, termed P-glycoprotein, has been shown to be responsible for cross-resistance to a broad range of structurally and functionally distinct cytotoxic agents. P-glycoprotein, encoded in humans by the mdr1 gene, functions as an energy-dependent efflux pump to exclude these

THE ROLE OF P-GLYCOPROTEIN IN RATIONAL PHARMACOTHERAPY IN CARDIOLOGY

Directory of Open Access Journals (Sweden)

A. V. Shulkin

2015-09-01

Full Text Available On the basis of the analysis of published data the role of P-glycoprotein, carrier protein, in rational pharmacotherapy in cardiology was shown on the example of its substrates – digoxin, antiplatelet agents and anticoagulants. Determination of C3435T polymorphism of multidrug resistance gene (MDR1, encoding P-glycoprotein, in pharmacotherapy with digoxin, antiplatelet drugs (clopidogrel tikagrelol, prasugrel and anticoagulants (dabigatran etexilate, rivaroxaban, edoxaban is not feasible in routine practice. Drug in- teractions have clinical implications for the efficacy and safety of pharmacotherapy in coadministration of these drugs with P-glycoprotein substrates, inducers and inhibitors.

Structures and Functions of Pestivirus Glycoproteins: Not Simply Surface Matters

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Fun-In Wang

2015-06-01

Full Text Available Pestiviruses, which include economically important animal pathogens such as bovine viral diarrhea virus and classical swine fever virus, possess three envelope glycoproteins, namely Erns, E1, and E2. This article discusses the structures and functions of these glycoproteins and their effects on viral pathogenicity in cells in culture and in animal hosts. E2 is the most important structural protein as it interacts with cell surface receptors that determine cell tropism and induces neutralizing antibody and cytotoxic T-lymphocyte responses. All three glycoproteins are involved in virus attachment and entry into target cells. E1-E2 heterodimers are essential for viral entry and infectivity. Erns is unique because it possesses intrinsic ribonuclease (RNase activity that can inhibit the production of type I interferons and assist in the development of persistent infections. These glycoproteins are localized to the virion surface; however, variations in amino acids and antigenic structures, disulfide bond formation, glycosylation, and RNase activity can ultimately affect the virulence of pestiviruses in animals. Along with mutations that are driven by selection pressure, antigenic differences in glycoproteins influence the efficacy of vaccines and determine the appropriateness of the vaccines that are currently being used in the field.

Importance of the short cytoplasmic domain of the feline immunodeficiency virus transmembrane glycoprotein for fusion activity and envelope glycoprotein incorporation into virions

International Nuclear Information System (INIS)

Celma, Cristina C.P.; Paladino, Monica G.; Gonzalez, Silvia A.; Affranchino, Jose L.

2007-01-01

The mature form of the envelope (Env) glycoprotein of lentiviruses is a heterodimer composed of the surface (SU) and transmembrane (TM) subunits. Feline immunodeficiency virus (FIV) possesses a TM glycoprotein with a cytoplasmic tail of approximately 53 amino acids which is unusually short compared with that of the other lentiviral glycoproteins (more than 100 residues). To investigate the relevance of the FIV TM cytoplasmic domain to Env-mediated viral functions, we characterized the biological properties of a series of Env glycoproteins progressively shortened from the carboxyl terminus. All the mutant Env proteins were efficiently expressed in feline cells and processed into the SU and TM subunits. Deletion of 5 or 11 amino acids from the TM C-terminus did not significantly affect Env surface expression, fusogenic activity or Env incorporation into virions, whereas removal of 17 or 23 residues impaired Env-mediated cell-to-cell fusion. Further truncation of the FIV TM by 29 residues resulted in an Env glycoprotein that was poorly expressed at the cell surface, exhibited only 20% of the wild-type Env fusogenic capacity and was inefficiently incorporated into virions. Remarkably, deletion of the TM C-terminal 35 or 41 amino acids restored or even enhanced Env biological functions. Indeed, these mutant Env glycoproteins bearing cytoplasmic domains of 18 or 12 amino acids were found to be significantly more fusogenic than the wild-type Env and were efficiently incorporated into virions. Interestingly, truncation of the TM cytoplasmic domain to only 6 amino acids did not affect Env incorporation into virions but abrogated Env fusogenicity. Finally, removal of the entire TM cytoplasmic tail or deletion of as many as 6 amino acids into the membrane-spanning domain led to a complete loss of Env functions. Our results demonstrate that despite its relatively short length, the FIV TM cytoplasmic domain plays an important role in modulating Env-mediated viral functions

Quantitative mass spectrometric analysis of glycoproteins combined with enrichment methods.

Science.gov (United States)

Ahn, Yeong Hee; Kim, Jin Young; Yoo, Jong Shin

2015-01-01

Mass spectrometry (MS) has been a core technology for high sensitive and high-throughput analysis of the enriched glycoproteome in aspects of quantitative assays as well as qualitative profiling of glycoproteins. Because it has been widely recognized that aberrant glycosylation in a glycoprotein may involve in progression of a certain disease, the development of efficient analysis tool for the aberrant glycoproteins is very important for deep understanding about pathological function of the glycoprotein and new biomarker development. This review first describes the protein glycosylation-targeting enrichment technologies mainly employing solid-phase extraction methods such as hydrizide-capturing, lectin-specific capturing, and affinity separation techniques based on porous graphitized carbon, hydrophilic interaction chromatography, or immobilized boronic acid. Second, MS-based quantitative analysis strategies coupled with the protein glycosylation-targeting enrichment technologies, by using a label-free MS, stable isotope-labeling, or targeted multiple reaction monitoring (MRM) MS, are summarized with recent published studies. © 2014 The Authors. Mass Spectrometry Reviews Published by Wiley Periodicals, Inc.

A Method for Determining the Content of Glycoproteins in Biological Samples

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Yang Gao

2016-11-01

Full Text Available The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG, was detected with Inductively coupled plasma mass spectrometry (ICP-MS. The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs.

Luminescence dating of paleolake deltas and glacial deposits in Garwood Valley, Antarctica: Implications for climate, Ross ice sheet dynamics, and paleolake duration

Science.gov (United States)

Levy, Joseph S.; Rittenour, Tammy M.; Fountain, Andrew G.; O'Connor, Jim E.

2017-01-01

The formation of perched deltas and other lacustrine deposits in the McMurdo Dry Valleys of Antarctica is widely considered to be evidence of valley-filling lakes dammed by the grounded Ross Sea ice sheet during the local Last Glacial Maximum, with lake drainage interpreted as a record of grounding line retreat. We used luminescence dating to determine the age of paleolake deltas and glacial tills in Garwood Valley, a coastal dry valley that opens to the Ross Sea. Luminescence ages are stratigraphically consistent with radiocarbon results from algal mats within the same delta deposits but suggest radiocarbon dates from lacustrine carbonates may overestimate deposit ages by thousands of years. Results suggest that late Holocene delta deposition into paleolake Howard in Garwood Valley persisted until ca. 3.5 ka. This is significantly younger than the date when grounded ice is thought to have retreated from the Ross Sea. Our evidence suggests that the local, stranded ice-cored till topography in Garwood Valley, rather than regional ice-sheet dynamics, may have controlled lake levels for some McMurdo Dry Valleys paleolakes. Age control from the supraglacial Ross Sea drift suggests grounding and up-valley advance of the Ross Sea ice sheet into Garwood valley during marine oxygen isotope stage (MIS) 4 (71–78 ka) and the local Last Glacial Maximum (9–10 ka). This work demonstrates the power of combining luminescence dating with existing radiocarbon data sets to improve understanding of the relationships among paleolake formation, glacial position, and stream discharge in response to climate change.

Glycoprotein of the wall of sycamore tissue-culture cells.

Science.gov (United States)

Heath, M F; Northcote, D H

1971-12-01

1. A glycoprotein containing a large amount of hydroxyproline is present in the cell walls of sycamore callus cells. This protein is insoluble and remained in the alpha-cellulose when a mild separation procedure was used to obtain the polysaccharide fractions of the wall. The glycoprotein contained a high proportion of arabinose and galactose. 2. Soluble glycopeptides were prepared from the alpha-cellulose fraction when peptide bonds were broken by hydrazinolysis. The soluble material was fractionated by gel filtration and one glycopeptide was further purified by electrophoresis; it had a composition of 10% hydroxyproline, 35% arabinose and 55% galactose, and each hydroxyproline residue carried a glycosyl radical so that the oligosaccharides on the glycopeptide had an average degree of polymerization of 9. 3. The extraction of the glycopeptides was achieved without cleavage of glycosyl bonds, so that the glycoprotein cannot act as a covalent cross-link between the major polysaccharides of the wall. 4. The wall protein approximates in conformation to polyhydroxyproline and therefore it probably has similar physicochemical properties to polyhydroxyproline. This is discussed in relation to the function of the glycoprotein and its effect on the physical and chemical nature of the wall.

Physical, chemical, and biological data collected during two research cruises in the Ross Sea, Antarctica from December 2004 to January 2005 and October to November 2005 for Ross Sea Sulfur Dynamics study (NODC Accession 0117322)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains transect data from two research cruises to the Ross Sea, Antarctica, aboard the RV Nathaniel B. Palmer (NBP) in December 2004 to January 2005...

Global identification of prokaryotic glycoproteins based on an Escherichia coli proteome microarray.

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Zong-Xiu Wang

Full Text Available Glycosylation is one of the most abundant protein posttranslational modifications. Protein glycosylation plays important roles not only in eukaryotes but also in prokaryotes. To further understand the roles of protein glycosylation in prokaryotes, we developed a lectin binding assay to screen glycoproteins on an Escherichia coli proteome microarray containing 4,256 affinity-purified E.coli proteins. Twenty-three E.coli proteins that bound Wheat-Germ Agglutinin (WGA were identified. PANTHER protein classification analysis showed that these glycoprotein candidates were highly enriched in metabolic process and catalytic activity classes. One sub-network centered on deoxyribonuclease I (sbcB was identified. Bioinformatics analysis suggests that prokaryotic protein glycosylation may play roles in nucleotide and nucleic acid metabolism. Fifteen of the 23 glycoprotein candidates were validated by lectin (WGA staining, thereby increasing the number of validated E. coli glycoproteins from 3 to 18. By cataloguing glycoproteins in E.coli, our study greatly extends our understanding of protein glycosylation in prokaryotes.

Thyroid hormone upregulates zinc-α2-glycoprotein production in the liver but not in adipose tissue.

Directory of Open Access Journals (Sweden)

Rafael Simó

Full Text Available Overproduction of zinc-α2-glycoprotein by adipose tissue is crucial in accounting for the lipolysis occurring in cancer cachexia of certain malignant tumors. The main aim of this study was to explore whether thyroid hormone could enhance zinc-α2-glycoprotein production in adipose tissue. In addition, the regulation of zinc-α2-glycoprotein by thyroid hormone in the liver was investigated. We performed in vitro (HepG2 cells and primary human adipocytes and in vivo (C57BL6/mice experiments addressed to examine the effect of thyroid hormone on zinc-α2-glycoprotein production (mRNA and protein levels in liver and visceral adipose tissue. We also measured the zinc-α2-glycoprotein serum levels in a cohort of patients before and after controlling their hyperthyroidism. Our results showed that thyroid hormone up-regulates zinc-α2-glycoprotein production in HepG2 cells in a dose-dependent manner. In addition, the zinc-α2-glycoprotein proximal promoter contains functional thyroid hormone receptor binding sites that respond to thyroid hormone treatment in luciferase reporter gene assays in HepG2 cells. Furthermore, zinc-α2-glycoprotein induced lipolysis in HepG2 in a dose-dependent manner. Our in vivo experiments in mice confirmed the up-regulation of zinc-α2-glycoprotein induced by thyroid hormone in the liver, thus leading to a significant increase in zinc-α2-glycoprotein circulating levels. However, thyroid hormone did not regulate zinc-α2-glycoprotein production in either human or mouse adipocytes. Finally, in patients with hyperthyroidism a significant reduction of zinc-α2-glycoprotein serum levels was detected after treatment but was unrelated to body weight changes. We conclude that thyroid hormone up-regulates the production of zinc-α2-glycoprotein in the liver but not in the adipose tissue. The neutral effect of thyroid hormones on zinc-α2-glycoprotein expression in adipose tissue could be the reason why zinc-α2-glycoprotein is not

Thyroid hormone upregulates zinc-α2-glycoprotein production in the liver but not in adipose tissue.

Science.gov (United States)

Simó, Rafael; Hernández, Cristina; Sáez-López, Cristina; Soldevila, Berta; Puig-Domingo, Manel; Selva, David M

2014-01-01

Overproduction of zinc-α2-glycoprotein by adipose tissue is crucial in accounting for the lipolysis occurring in cancer cachexia of certain malignant tumors. The main aim of this study was to explore whether thyroid hormone could enhance zinc-α2-glycoprotein production in adipose tissue. In addition, the regulation of zinc-α2-glycoprotein by thyroid hormone in the liver was investigated. We performed in vitro (HepG2 cells and primary human adipocytes) and in vivo (C57BL6/mice) experiments addressed to examine the effect of thyroid hormone on zinc-α2-glycoprotein production (mRNA and protein levels) in liver and visceral adipose tissue. We also measured the zinc-α2-glycoprotein serum levels in a cohort of patients before and after controlling their hyperthyroidism. Our results showed that thyroid hormone up-regulates zinc-α2-glycoprotein production in HepG2 cells in a dose-dependent manner. In addition, the zinc-α2-glycoprotein proximal promoter contains functional thyroid hormone receptor binding sites that respond to thyroid hormone treatment in luciferase reporter gene assays in HepG2 cells. Furthermore, zinc-α2-glycoprotein induced lipolysis in HepG2 in a dose-dependent manner. Our in vivo experiments in mice confirmed the up-regulation of zinc-α2-glycoprotein induced by thyroid hormone in the liver, thus leading to a significant increase in zinc-α2-glycoprotein circulating levels. However, thyroid hormone did not regulate zinc-α2-glycoprotein production in either human or mouse adipocytes. Finally, in patients with hyperthyroidism a significant reduction of zinc-α2-glycoprotein serum levels was detected after treatment but was unrelated to body weight changes. We conclude that thyroid hormone up-regulates the production of zinc-α2-glycoprotein in the liver but not in the adipose tissue. The neutral effect of thyroid hormones on zinc-α2-glycoprotein expression in adipose tissue could be the reason why zinc-α2-glycoprotein is not related to weight

3,3′,4,4′,5-Pentachlorobiphenyl Inhibits Drug Efflux Through P-Glycoprotein in KB-3 Cells Expressing Mutant Human P-Glycoprotein

Directory of Open Access Journals (Sweden)

Hiroshi Fujise

2004-01-01

Full Text Available The effects on the drug efflux of 3,3′,4,4′,5-pentachlorobiphenyl (PCB-126, the most toxic of all coplanar polychlorinated biphenyls (Co-PCBs, were examined in KB-3 cells expressing human wild-type and mutant P-glycoprotein in which the 61st amino acid was substituted for serine or phenylalanine (KB3-Phe61. In the cells expressing P-glycoproteins, accumulations of vinblastine and colchicine decreased form 85% to 92% and from 62% to 91%, respectively, and the drug tolerances for these chemicals were increased. In KB3-Phe61, the decreases in drug accumulation were inhibited by adding PCB-126 in a way similar to that with cyclosporine A: by adding 1 μM PCB-126, the accumulations of vinblastine and colchicine increased up to 3.3- and 2.3-fold, respectively. It is suggested that PCB-126 decreased the drug efflux by inhibiting the P-glycoprotein in KB3-Phe61. Since there were various P-glycoproteins and many congeners of Co-PCBs, this inhibition has to be considered a new cause of the toxic effects of Co-PCBs.

Prediction of conserved sites and domains in glycoproteins B, C and D of herpes viruses.

Science.gov (United States)

Rasheed, Muhammad Asif; Ansari, Abdur Rahman; Ihsan, Awais; Navid, Muhammad Tariq; Ur-Rehman, Shahid; Raza, Sohail

2018-03-01

Glycoprotein B (gB), C (gC) and D (gD) of herpes simplex virus are implicated in virus adsorption and penetration. The gB, gC and gD are glycoproteins for different processes of virus binding and attachment to the host cells. Moreover, their expression is necessary and sufficient to induce cell fusion in the absence of other glycoproteins. Egress of herpes simplex virus (HSV) and other herpes viruses from cells involves extensive modification of cellular membranes and sequential envelopment, de-envelopment and re-envelopment steps. Viral glycoproteins are important in these processes, and frequently two or more glycoproteins can largely suffice in any step. Hence, we target the 3 important glycoproteins (B, C and D) of eight different herpes viruses of different species. These species include human (HSV1 and 2), bovine (BHV1), equine (EHV1 and 4), chicken (ILT1 and MDV2) and pig (PRV1). By applying different bioinformatics tools, we highlighted the conserved sites in these glycoproteins which might be most significant regarding attachment and infection of the viruses. Moreover the conserved domains in these glycoproteins are also highlighted. From this study, we will able to analyze the role of different viral glycoproteins of different species during herpes virus adsorption and penetration. Moreover, this study will help to construct the antivirals that target the glycoproteins of different herpes viruses. Copyright © 2018 Elsevier Ltd. All rights reserved.

Distribution of Ra-226 in the Ross Sea, Antarctica

International Nuclear Information System (INIS)

Bettoli, M.G.; Cantelli, L.; Queirazza, G.; Roveri, M.; Tositti, L.; Tubertini, O.; Valcher, S.

1996-01-01

An improved procedure for the determination of Rn-222 and Ra-226 in seawater developed for easier on-board operations is presented. Data on the radioactive disequilibrium between the mentioned radionuclides as directly determined in the field can be greatly helpful in the study of the gas-exchange processes at the air-sea interface, especially as far as the Antarctic Ocean is concerned. The method employed has been preliminary tested in laboratory on a set of seawater samples collected in the Ross Sea and Ra-226 data collected are discussed and compared to literature data. (UK)

Astronaut Ross Approaches Assembly Concept for Construction of Erectable Space Structure (ACCESS)

Science.gov (United States)

1999-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia, and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo, astronaut Ross, perched on the Manipulator Foot Restraint (MFR) approaches the erected ACCESS. The primary objective of these experiments was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Assessing Competition with the Panzar-Rosse Model: The Role of Scale, Costs, and Equilibrium

NARCIS (Netherlands)

Bikker, J.A.|info:eu-repo/dai/nl/06912261X; Shaffer, S.; Spierdijk, L.

2009-01-01

The Panzar-Rosse test has been widely applied to assess competitive conduct, often in specifcations controlling for firm scale or using a price equation. We show that neither a price equation nor a scaled revenue function yields a valid measure for competitive conduct. Moreover, even an unscaled

Ross E. Baker, DC: A Canadian chiropractic survivor.

Science.gov (United States)

Brown, Douglas M

2014-03-01

This paper is an historical biography of a fortunate man. It begins with a glimpse of Ross E. Baker's origins in south-western Ontario, watches him going to school and working in Hamilton before joining the Canadian Army and shipping off to Europe to fight in the Second World War. At War's end, the article picks up Dr. Baker as he comes home, starts a family, becomes a chiropractor and sustains a viable practice. Now in the twilight of life, the good doctor is last seen content with his retirement, spending days at his cottage property, reviewing his memoirs and reflecting on the tumult, terror and eventual triumph of the D-Day landing at Normandy.

Glycoprotein and proteoglycan techniques

International Nuclear Information System (INIS)

Beeley, J.G.

1985-01-01

The aim of this book is to describe techniques which can be used to answer some of the basic questions about glycosylated proteins. Methods are discussed for isolation, compositional analysis, and for determination of the primary structure of carbohydrate units and the nature of protein-carbohydrate linkages of glycoproteins and proteoglycans. High resolution NMR is considered, as well as radioactive labelling techniques. (Auth.)

HIV-1 envelope glycoprotein

Science.gov (United States)

Caulfield, Michael; Cupo, Albert; Dean, Hansi; Hoffenberg, Simon; King, C. Richter; Klasse, P. J.; Marozsan, Andre; Moore, John P.; Sanders, Rogier W.; Ward, Andrew; Wilson, Ian; Julien, Jean-Philippe

2017-08-22

The present application relates to novel HIV-1 envelope glycoproteins, which may be utilized as HIV-1 vaccine immunogens, and antigens for crystallization, electron microscopy and other biophysical, biochemical and immunological studies for the identification of broad neutralizing antibodies. The present invention encompasses the preparation and purification of immunogenic compositions, which are formulated into the vaccines of the present invention.

A NEW ANALYSIS OF THE TWO CLASSICAL ZZ CETI WHITE DWARFS GD 165 AND ROSS 548. II. SEISMIC MODELING

International Nuclear Information System (INIS)

Giammichele, N.; Fontaine, G.; Brassard, P.; Charpinet, S.

2016-01-01

We present the second of a two-part seismic analysis of the bright, hot ZZ Ceti stars GD 165 and Ross 548. In this second part, we report the results of detailed searches in parameter space for identifying an optimal model for each star that can account well for the observed periods, while being consistent with the spectroscopic constraints derived in our first paper. We find optimal models for each target that reproduce the six observed periods well within ∼0.3% on the average. We also find that there is a sensitivity on the core composition for Ross 548, while there is practically none for GD 165. Our optimal model of Ross 548, with its thin envelope, indeed shows weight functions for some confined modes that extend relatively deep into the interior, thus explaining the sensitivity of the period spectrum on the core composition in that star. In contrast, our optimal seismic model of its spectroscopic sibling, GD 165 with its thick envelope, does not trap/confine modes very efficiently, and we find weight functions for all six observed modes that do not extend into the deep core, hence accounting for the lack of sensitivity in that case. Furthermore, we exploit after the fact the observed multiplet structure that we ascribe to rotation. We are able to map the rotation profile in GD 165 (Ross 548) over the outermost ∼20% (∼5%) of its radius, and we find that the profile is consistent with solid-body rotation

Evaluating indices of lipid and protein content in lesser snow and Ross's geese during spring migration

Science.gov (United States)

Webb, Elisabeth B.; Fowler, Drew N.; Woodall, Brendan A.; Vrtiska, Mark P.

2018-01-01

Assessing nutrient stores in avian species is important for understanding the extent to which body condition influences success or failure in life‐history events. We evaluated predictive models using morphometric characteristics to estimate total body lipids (TBL) and total body protein (TBP), based on traditional proximate analyses, in spring migrating lesser snow geese (Anser caerulescens caerulescens) and Ross's geese (A. rossii). We also compared performance of our lipid model with a previously derived predictive equation for TBL developed for nesting lesser snow geese. We used external and internal measurements on 612 lesser snow and 125 Ross's geese collected during spring migration in 2015 and 2016 within the Central and Mississippi flyways to derive and evaluate predictive models. Using a validation data set, our best performing lipid model for snow geese better predicted TBL (root mean square error [RMSE] of 23.56) compared with a model derived from nesting individuals (RMSE = 48.60), suggesting the importance of season‐specific models for accurate lipid estimation. Models that included body mass and abdominal fat deposit best predicted TBL determined by proximate analysis in both species (lesser snow goose, R2 = 0.87, RMSE = 23.56: Ross's geese, R2 = 0.89, RMSE = 13.75). Models incorporating a combination of external structural measurements in addition to internal muscle and body mass best predicted protein values (R2 = 0.85, RMSE = 19.39 and R2 = 0.85, RMSE = 7.65, lesser snow and Ross's geese, respectively), but protein models including only body mass and body size were also competitive and provided extended utility to our equations for field applications. Therefore, our models indicated the importance of specimen dissection and measurement of the abdominal fat pad to provide the most accurate lipid estimates and provide alternative dissection‐free methods for estimating protein.

76 FR 71082 - Strata Energy, Inc., Ross Uranium Recovery Project; New Source Material License Application...

Science.gov (United States)

2011-11-16

... NUCLEAR REGULATORY COMMISSION [Docket No. 40-9091; NRC-2011-0148] Strata Energy, Inc., Ross Uranium Recovery Project; New Source Material License Application; Notice of Intent To Prepare a... intent to prepare a supplemental environmental impact statement. SUMMARY: Strata Energy, Inc. (Strata...

TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.

Directory of Open Access Journals (Sweden)

Stephanie Jemielity

2013-03-01

Full Text Available Human T-cell Immunoglobulin and Mucin-domain containing proteins (TIM1, 3, and 4 specifically bind phosphatidylserine (PS. TIM1 has been proposed to serve as a cellular receptor for hepatitis A virus and Ebola virus and as an entry factor for dengue virus. Here we show that TIM1 promotes infection of retroviruses and virus-like particles (VLPs pseudotyped with a range of viral entry proteins, in particular those from the filovirus, flavivirus, New World arenavirus and alphavirus families. TIM1 also robustly enhanced the infection of replication-competent viruses from the same families, including dengue, Tacaribe, Sindbis and Ross River viruses. All interactions between TIM1 and pseudoviruses or VLPs were PS-mediated, as demonstrated with liposome blocking and TIM1 mutagenesis experiments. In addition, other PS-binding proteins, such as Axl and TIM4, promoted infection similarly to TIM1. Finally, the blocking of PS receptors on macrophages inhibited the entry of Ebola VLPs, suggesting that PS receptors can contribute to infection in physiologically relevant cells. Notably, infection mediated by the entry proteins of Lassa fever virus, influenza A virus and SARS coronavirus was largely unaffected by TIM1 expression. Taken together our data show that TIM1 and related PS-binding proteins promote infection of diverse families of enveloped viruses, and may therefore be useful targets for broad-spectrum antiviral therapies.

Ross operation in children and young adults: the Alder Hey case series

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Pozzi Marco

2004-04-01

Full Text Available Abstract Background The ideal prosthesis for aortic valve replacement in children and young adults has not been found yet. In recent years there has been a renewed interest in the replacement of aortic valve with the pulmonary autograft owing to its advantages of lack of anticoagulation, potential for growth and excellent haemodynamic performance. The purpose of this study was to review our institutional experience at Alder Hey hospital with the Ross procedure in children and young adults. Methods From November 1996 to September 2003, 38 patients (mean age, 13.1 ± 5.7 years underwent the Ross procedure for various aortic valve diseases using the root replacement technique. Clinical and echocardiographic follow-up was performed early (within 30 days, 3 to 6 months, and yearly after surgery. Medical records of all patients were reviewed retrospectively. Results There was 1 perioperative death. The patients were followed-up for a median interval of 36 months and up to 7 years. One patient died 3 years after surgery secondary to ventricular arrhythmia with overall mortality of 5.3%. Actuarial survival at 7 years was 94 ± 2.5% and there was 100% freedom from reoperation for autograft valve dysfunction or any other cause. Balloon dilatation was required in 2 patients for pulmonary homograft stenosis. The haemodynamics at the latest follow-up were also similar to those at the time of discharge after surgery. There was no progression in the degree of aortic regurgitation for 11 patients with trivial and 3 with mild regurgitation. Conclusion Our experience demonstrates that Ross operation is an attractive option for aortic valve replacement in children and young adults. Not only can the operation be accomplished with a low operative risk but the valve function stays normal over a long period of time with minimal alteration in lifestyle and no need for repeated operations to replace the valve as a result of somatic growth of the children.

Influence of a yeast fermented product on the serum levels of the mannan-binding lectin and the antibodies against the Newcastle disease virus in Ross broilers

DEFF Research Database (Denmark)

Cortés-Coronado, R F; Gómez-Rosales, S; de L Angeles, M

2017-01-01

The objective of this research was to evaluate the serum concentrations of mannan-binding lectin (MBL) at different ages in Ross broilers fed increasing amounts of a yeast-fermented product (YFP) and inoculated with a vaccine against Newcastle disease virus (NDV). Eighty mixed Ross B308 broilers...

Crystallization and preliminary X-ray analysis of Chandipura virus glycoprotein G

International Nuclear Information System (INIS)

Baquero, Eduard; Buonocore, Linda; Rose, John K.; Bressanelli, Stéphane; Gaudin, Yves; Albertini, Aurélie A.

2012-01-01

Chandipura virus glycoprotein ectodomain (Gth) was purified and crystallized at pH 7.5. X-ray diffraction data set was collected to a resolution of 3.1 Å. Fusion in members of the Rhabdoviridae virus family is mediated by the G glycoprotein. At low pH, the G glycoprotein catalyzes fusion between viral and endosomal membranes by undergoing a major conformational change from a pre-fusion trimer to a post-fusion trimer. The structure of the G glycoprotein from vesicular stomatitis virus (VSV G), the prototype of Vesiculovirus, has recently been solved in its trimeric pre-fusion and post-fusion conformations; however, little is known about the structural details of the transition. In this work, a soluble form of the ectodomain of Chandipura virus G glycoprotein (CHAV G th ) was purified using limited proteolysis of purified virus; this soluble ectodomain was also crystallized. This protein shares 41% amino-acid identity with VSV G and thus its structure could provide further clues about the structural transition of rhabdoviral glycoproteins induced by low pH. Crystals of CHAV G th obtained at pH 7.5 diffracted X-rays to 3.1 Å resolution. These crystals belonged to the orthorhombic space group P2 1 2 1 2, with unit-cell parameters a = 150.3, b = 228.2, c = 78.8 Å. Preliminary analysis of the data based on the space group and the self-rotation function indicated that there was no trimeric association of the protomers. This unusual oligomeric status could result from the presence of fusion intermediates in the crystal

Atmospheric Boundary Layer Dynamics Near Ross Island and Over West Antarctica.

Science.gov (United States)

Liu, Zhong

The atmospheric boundary layer dynamics near Ross Island and over West Antarctica has been investigated. The study consists of two parts. The first part involved the use of data from ground-based remote sensing equipment (sodar and RASS), radiosondes, pilot balloons, automatic weather stations, and NOAA AVHRR satellite imagery. The second part involved the use of a high resolution boundary layer model coupled with a three-dimensional primitive equation mesoscale model to simulate the observed atmospheric boundary layer winds and temperatures. Turbulence parameters were simulated with an E-epsilon turbulence model driven by observed winds and temperatures. The observational analysis, for the first time, revealed that the airflow passing through the Ross Island area is supplied mainly by enhanced katabatic drainage from Byrd Glacier and secondarily drainage from Mulock and Skelton glaciers. The observed diurnal variation of the blocking effect near Ross Island is dominated by the changes in the upstream katabatic airflow. The synthesized analysis over West Antarctica found that the Siple Coast katabatic wind confluence zone consists of two superimposed katabatic airflows: a relatively warm and more buoyant katabatic flow from West Antarctica overlies a colder and less buoyant katabatic airflow from East Antarctica. The force balance analysis revealed that, inside the West Antarctic katabatic wind zone, the pressure gradient force associated with the blocked airflow against the Transantarctic Mountains dominates; inside the East Antarctic katabatic wind zone, the downslope buoyancy force due to the cold air overlying the sloping terrain is dominant. The analysis also shows that these forces are in geostrophic balance with the Coriolis force. An E-epsilon turbulence closure model is used to simulate the diurnal variation of sodar backscatter. The results show that the model is capable of qualitatively capturing the main features of the observed sodar backscatter. To

Synthetic glycopeptides and glycoproteins with applications in biological research

Directory of Open Access Journals (Sweden)

Ulrika Westerlind

2012-05-01

Full Text Available Over the past few years, synthetic methods for the preparation of complex glycopeptides have been drastically improved. The need for homogenous glycopeptides and glycoproteins with defined chemical structures to study diverse biological phenomena further enhances the development of methodologies. Selected recent advances in synthesis and applications, in which glycopeptides or glycoproteins serve as tools for biological studies, are reviewed. The importance of specific antibodies directed to the glycan part, as well as the peptide backbone has been realized during the development of synthetic glycopeptide-based anti-tumor vaccines. The fine-tuning of native chemical ligation (NCL, expressed protein ligation (EPL, and chemoenzymatic glycosylation techniques have all together enabled the synthesis of functional glycoproteins. The synthesis of structurally defined, complex glycopeptides or glyco-clusters presented on natural peptide backbones, or mimics thereof, offer further possibilities to study protein-binding events.

The glycoproteins of Marburg and Ebola virus and their potential roles in pathogenesis.

Science.gov (United States)

Feldmann, H; Volchkov, V E; Volchkova, V A; Klenk, H D

1999-01-01

Filoviruses cause systemic infections that can lead to severe hemorrhagic fever in human and non-human primates. The primary target of the virus appears to be the mononuclear phagocytic system. As the virus spreads through the organism, the spectrum of target cells increases to include endothelial cells, fibroblasts, hepatocytes, and many other cells. There is evidence that the filovirus glycoprotein plays an important role in cell tropism, spread of infection, and pathogenicity. Biosynthesis of the glycoprotein forming the spikes on the virion surface involves cleavage by the host cell protease furin into two disulfide linked subunits GP1 and GP2. GP1 is also shed in soluble form from infected cells. Different strains of Ebola virus show variations in the cleavability of the glycoprotein, that may account for differences in pathogenicity, as has been observed with influenza viruses and paramyxoviruses. Expression of the spike glycoprotein of Ebola virus, but not of Marburg virus, requires transcriptional editing. Unedited GP mRNA yields the nonstructural glycoprotein sGP, which is secreted extensively from infected cells. Whether the soluble glycoproteins GP1 and sGP interfere with the humoral immune response and other defense mechanisms remains to be determined.

Vacuum-field solutions of Ross and Sen-Dunn theories of gravitation

International Nuclear Information System (INIS)

Krori, K.D.; Nandy, D.

1978-01-01

Vacuum-field solutions of Ross (Phys. Rev.; D5:284 (1972)) and Sen-Dunn (J. Math. Phys.; 12:578 (1971)) theories of gravitation have been obtained with the aid of a Friedmann-type metric. Non-static solutions are found showing that the Birkhoff theorem holds for neither theory. It has been observed that the two theories have a limited scope for vacuum solution as against the Brans-Dicke theory. Mach's principle, however, holds for both the theories. (author)

Three-dimensionally Functionalized Reverse Phase Glycoprotein Array for Cancer Biomarker Discovery and Validation

OpenAIRE

Pan, Li; Aguilar, Hillary Andaluz; Wang, Linna; Iliuk, Anton; Tao, W. Andy

2016-01-01

Glycoproteins have vast structural diversity which plays an important role in many biological processes and have great potential as disease biomarkers. Here we report a novel functionalized reverse phase protein array (RPPA), termed polymer-based reverse phase GlycoProtein Array (polyGPA), to specifically capture and profile glycoproteomes, and validate glycoproteins. Nitrocellulose membrane functionalized with globular hydroxyaminodendrimers was used to covalently capture pre-oxidized glycan...

A Battle of Words: "Dignity" and "Peace" in the Writings of Elisabeth Kübler-Ross.

Science.gov (United States)

Burnier, Daniel

2017-04-01

This article analyzes the writings of Elisabeth Kübler-Ross through the discursive lens of the phrase "dying with dignity." For her, the phrase meant allowing someone to die comfortably his/her own death. This phrase has to be understood in relationship with the final "stage of acceptance" of her model. Describing this key part of her well-known scientific output, she often used, in the early 1970s, the phrase "dying in peace and dignity." An evaluation of the evidence suggests that because the concept of dignity was co-opted by the pro-euthanasia movement during this decade, the language of dignity was little by little abandoned by her. In later years, only "peace" survived from her favorite expression. Although this concept of peace remains present to the end in all Kübler-Ross writings, the pro-euthanasia movement has also started to speak the language of peace.

Diet and trophic niche of Antarctic silverfish Pleuragramma antarcticum in the Ross Sea, Antarctica.

Science.gov (United States)

Pinkerton, M H; Forman, J; Bury, S J; Brown, J; Horn, P; O'Driscoll, R L

2013-01-01

The diet of Antarctic silverfish Pleuragramma antarcticum was evaluated by examining stomach contents of specimens collected in the Ross Sea (71°-77° S; 165°-180° E) in January to March 2008. Pleuragramma antarcticum (50-236 mm standard length, L(S)) and prey items were analysed for stable-isotopic composition of carbon and nitrogen. According to index of relative importance (I(RI) ), which incorporates frequency of occurrence, mass and number of prey items, the most important prey items were copepods (81%I(RI) over all specimens), predominantly Metridia gerlachei and Paraeuchaeta sp., with krill and fishes having low I(RI) (2·2 and 5·6%I(RI) overall). According to mass of prey (M) in stomachs, however, fishes (P. antarcticum and myctophids) and krill dominated overall diet (48 and 22%M, respectively), with copepods being a relatively minor constituent of overall diet by mass (9·9%M). Piscivory by P. antarcticum occurred mainly in the extreme south-west of the region and near the continental slope. Krill identified to species level in P. antarcticum stomachs were predominantly Euphausia superba (14·1%M) with some Euphausia crystallophorias (4·8%M). Both DistLM modelling (PRIMER-permanova+) on stomach contents (by I(RI)) and stepwise generalized linear modelling on stable isotopes showed that L(S) and location were significant predictors of P. antarcticum diet. Postlarval P. antarcticum (50-89 mm L(S)) consumed exclusively copepods. Juvenile P. antarcticum (90-151 mm L(S)) consumed predominantly krill and copepods by mass (46 and 30%M, respectively). Small adult P. antarcticum (152-178 mm L(S)) consumed krill, fishes and copepods (37, 36 and 15%M, respectively). Large adult P. antarcticum (179-236 mm L(S)) consumed predominantly fishes and krill (55 and 17%M, respectively), especially in the north (near the Ross Sea slope) and in the SW Ross Sea. Amphipods were occasionally important prey items for P. antarcticum (western Ross Sea, 39%M). General

Multi-Decadal Averages of Basal Melt for Ross Ice Shelf, Antarctica Using Airborne Observations

Science.gov (United States)

Das, I.; Bell, R. E.; Tinto, K. J.; Frearson, N.; Kingslake, J.; Padman, L.; Siddoway, C. S.; Fricker, H. A.

2017-12-01

Changes in ice shelf mass balance are key to the long term stability of the Antarctic Ice Sheet. Although the most extensive ice shelf mass loss currently is occurring in the Amundsen Sea sector of West Antarctica, many other ice shelves experience changes in thickness on time scales from annual to ice age cycles. Here, we focus on the Ross Ice Shelf. An 18-year record (1994-2012) of satellite radar altimetry shows substantial variability in Ross Ice Shelf height on interannual time scales, complicating detection of potential long-term climate-change signals in the mass budget of this ice shelf. Variability of radar signal penetration into the ice-shelf surface snow and firn layers further complicates assessment of mass changes. We investigate Ross Ice Shelf mass balance using aerogeophysical data from the ROSETTA-Ice surveys using IcePod. We use two ice-penetrating radars; a 2 GHz unit that images fine-structure in the upper 400 m of the ice surface and a 360 MHz radar to identify the ice shelf base. We have identified internal layers that are continuous along flow from the grounding line to the ice shelf front. Based on layer continuity, we conclude that these layers must be the horizons between the continental ice of the outlet glaciers and snow accumulation once the ice is afloat. We use the Lagrangian change in thickness of these layers, after correcting for strain rates derived using modern day InSAR velocities, to estimate multidecadal averaged basal melt rates. This method provides a novel way to quantify basal melt, avoiding the confounding impacts of spatial and short-timescale variability in surface accumulation and firn densification processes. Our estimates show elevated basal melt rates (> -1m/yr) around Byrd and Mullock glaciers within 100 km from the ice shelf front. We also compare modern InSAR velocity derived strain rates with estimates from the comprehensive ground-based RIGGS observations during 1973-1978 to estimate the potential magnitude of

Trends in the Breeding Population of Adélie Penguins in the Ross Sea, 1981–2012: A Coincidence of Climate and Resource Extraction Effects

Science.gov (United States)

Lyver, Phil O’B.; Barron, Mandy; Barton, Kerry J.; Ainley, David G.; Pollard, Annie; Gordon, Shulamit; McNeill, Stephen; Ballard, Grant; Wilson, Peter R.

2014-01-01

Measurements of the size of Adélie penguin (Pygoscelis adeliae) colonies of the southern Ross Sea are among the longest biologic time series in the Antarctic. We present an assessment of recent annual variation and trends in abundance and growth rates of these colonies, adding to the published record not updated for more than two decades. High angle oblique aerial photographic surveys of colonies were acquired and penguins counted for the breeding seasons 1981–2012. In the last four years the numbers of Adélie penguins in the Ross and Beaufort Island colonies (southern Ross Sea metapopulation) reached their highest levels since aerial counts began in 1981. Results indicated that 855,625 pairs of Adélie penguins established breeding territories in the western Ross Sea, with just over a quarter (28%) of those in the southern portion, constituting a semi-isolated metapopulation (three colonies on Ross Island, one on nearby Beaufort Island). The southern population had a negative per capita growth rate of −0.019 during 1981–2000, followed by a positive per capita growth rate of 0.067 for 2001–2012. Colony growth rates for this metapopulation showed striking synchrony through time, indicating that large-scale factors influenced their annual growth. In contrast to the increased colony sizes in the southern population, the patterns of change among colonies of the northern Ross Sea were difficult to characterize. Trends were similar to southern colonies until the mid-1990s, after which the signal was lost owing to significantly reduced frequency of surveys. Both climate factors and recovery of whale populations likely played roles in the trends among southern colonies until 2000, after which depletion of another trophic competitor, the Antarctic toothfish (Dissostichus mawsoni), may explain the sharp increasing trend evident since then. PMID:24621601

Filamentous fungi as production organisms for glycoproteins of bio-medical interest

NARCIS (Netherlands)

Maras, M.; Die, I. van; Contreras, R.; Hondel, C.A.M.J.J. van den

1999-01-01

Filamentous fungi are commonly used in the fermentation industry for large scale production of glycoproteins. Several of these proteins can be produced in concentrations up to 20-40 g per litre. The production of heterologous glycoproteins is at least one or two orders of magnitude lower but

The surface climatology of the Ross Ice Shelf Antarctica.

Science.gov (United States)

Costanza, Carol A; Lazzara, Matthew A; Keller, Linda M; Cassano, John J

2016-12-01

The University of Wisconsin-Madison Antarctic Automatic Weather Station (AWS) project has been making meteorological surface observations on the Ross Ice Shelf (RIS) for approximately 30 years. This network offers the most continuous set of routine measurements of surface meteorological variables in this region. The Ross Island area is excluded from this study. The surface climate of the RIS is described using the AWS measurements. Temperature, pressure, and wind data are analysed on daily, monthly, seasonal, and annual time periods for 13 AWS across the RIS. The AWS are separated into three representative regions - central, coastal, and the area along the Transantarctic Mountains - in order to describe specific characteristics of sections of the RIS. The climatology describes general characteristics of the region and significant changes over time. The central AWS experiences the coldest mean temperature, and the lowest resultant wind speed. These AWSs also experience the coldest potential temperatures with a minimum of 209.3 K at Gill AWS. The AWS along the Transantarctic Mountains experiences the warmest mean temperature, the highest mean sea-level pressure, and the highest mean resultant wind speed. Finally, the coastal AWS experiences the lowest mean pressure. Climate indices (MEI, SAM, and SAO) are compared to temperature and pressure data of four of the AWS with the longest observation periods, and significant correlation is found for most AWS in sea-level pressure and temperature. This climatology study highlights characteristics that influence the climate of the RIS, and the challenges of maintaining a long-term Antarctic AWS network. Results from this effort are essential for the broader Antarctic meteorology community for future research.

Co-treatment by docetaxel and vinblastine breaks down P-glycoprotein mediated chemo-resistance

Directory of Open Access Journals (Sweden)

Mahsa Mohseni

2016-03-01

Results: Combination treatment of the cells with docetaxel and vinblastine decreased the IC50 values for docetaxel from (30±3.1 to (15±2.6 nM and for vinblastine from (30±5.9 to (5±5.6 nM (P≤0.05.               P-glycoprotein mRNA expression level showed a significant up-regulation in the cells incubated with each drug alone (P≤0.001. Incubation of the cells with combined concentrations of both agents neutralized P-glycoprotein overexpression (P≤0.05. Adding verapamil, a P-glycoprotein inhibitor caused a further increase in the percentage of apoptotic cells when the cells were treated with both agents.  Conclusion:Our results suggest that combination therapy along with P-glycoprotein inhibition can be considered as a novel approach to improve the efficacy of chemotherapeutics in cancer patients with high P-glycoprotein expression.

Glycoproteins and sialyl transferase of human B lymphoblastoid cell lines

International Nuclear Information System (INIS)

Lui, S.W.L.; Ng, M.H.

1980-01-01

We used two radiolabeling methods to study glycoproteins on the surface of lymphoblastoid cells. One of the methods affects tritiation of residues which are oxidized with galactose oxidase and the other causes tritiation of neuraminic acid residues. This approach was shown to allow a better resolution of cell surface glycoproteins than if either method were used alone. Glycoproteins of B 1 - 19 cells which harbor the Epstein-Barr virus genomes were compared with those of its parental cell line, BJAB, which does not harbor the viral genomes. These studies did not reveal a unique viral protein. A 28,000 mol. wt. glycoprotein was found to be the most prominent neuraminic acidlabeled product of B 1 - 19 cells and also of the two other cell lines, Raji and Ly38, which harbor the EBV genomes. A similar molecular weight species from BJAB cells identified by galactose oxidase labeling might be deficient in neuraminic acid residues as it was poorly labeled by the periodate oxidation method. The neuraminic acid content and level of sialyl transferase of BJAB cells were found to be lower than those of the other cell lines studied. (auth.)

Effects of chronic ethanol administration on hepatic glycoprotein secretion in the rat

International Nuclear Information System (INIS)

Sorrell, M.F.; Nauss, J.M.; Donohue, T.M. Jr.; Tuma, D.J.

1983-01-01

The effects of chronic ethanol feeding on protein and glycoprotein synthesis and secretion were studied in rat liver slices. Liver slices from rats fed ethanol for 4-5 wk showed a decreased ability to incorporate [ 14 C]glucosamine into medium trichloracetic acid-precipitable proteins when compared to the pair-fed controls; however, the labeling of hepatocellular glycoproteins was unaffected by chronic ethanol treatment. Immunoprecipitation of radiolabeled secretory (serum) glycoproteins with antiserum against rat serum proteins showed a similar marked inhibition in the appearance of glucosamine-labeled proteins in the medium of slices from ethanol-fed rats. Minimal effects, however, were noted in the labeling of intracellular secretory glycoproteins. Protein synthesis, as determined by measuring [ 14 C]leucine incorporation into medium and liver proteins, was decreased in liver slices from ethanol-fed rats as compared to the pair-fed controls. This was the case for both total proteins as well as immunoprecipitable secretory proteins, although the labeling of secretory proteins retained in the liver slices was reduced to a lesser extent than total radiolabeled hepatic proteins. When the terminal sugar, [ 14 C]fucose, was employed as a precursor in order to more closely focus on the final steps of hepatic glycoprotein secretion, liver slices obtained from chronic ethanol-fed rats exhibited impaired secretion of fucose-labeled proteins into the medium. When ethanol (5 or 10 mM) was added to the incubation medium containing liver slices from the ethanol-fed rats, the alterations in protein and glycoprotein synthesis and secretion caused by the chronic ethanol treatment were further potentiated. The results of this study indicate that liver slices prepared from chronic ethanol-fed rats exhibit both impaired synthesis and secretion of proteins and glycoproteins, and these defects are further potentiated by acute ethanol administration

Identification, isolation, and N-terminal sequencing of style glycoproteins associated with self-incompatibility in Nicotiana alata.

Science.gov (United States)

Jahnen, W; Batterham, M P; Clarke, A E; Moritz, R L; Simpson, R J

1989-05-01

S-Gene-associated glycoproteins (S-glycoproteins) from styles of Nicotiana alata, identified by non-equilibrium two-dimensional electrophoresis, were purified by cation exchange fast protein liquid chromatography with yields of 0.5 to 8 micrograms of protein per style, depending on the S-genotype of the plant. The method relies on the highly basic nature of the S-glycoproteins. The elution profiles of the different S-glycoproteins from the fast protein liquid chromatography column were characteristic of each S-glycoprotein, and could be used to establish the S-genotype of plants in outbreeding populations. In all cases, the S-genotype predicted from the style protein profile corresponded to that predicted from DNA gel blot analysis using S-allele-specific DNA probes and to that established by conventional breeding tests. Amino-terminal sequences of five purified S-glycoproteins showed a high degree of homology with the previously published sequences of N. alata and Lycopersicon esculentum S-glycoproteins.

"All this that has happened to me shouldn't happen to nobody else": Loretta Ross and the Women of Color Reproductive Freedom Movement of the 1980s.

Science.gov (United States)

Nelson, Jennifer

2010-01-01

Loretta Ross exemplifies women of color feminist participation in and transformation of the women's health movement of the 1970s and 1980s. Ross helped build a women's health movement that by the late 1980s made the demands of women of color central. This movement was attractive to many women of color who had rejected the collapse of a broader women's health movement into the abortion rights movement as too narrowly focused. Many women of color activists, including Ross, argued that the emphasis on abortion rights and choice failed to address the linked socioeconomic and community health issues confronted by many women of color and poor women. Ross's work spurred coalition building among white women and women of color that focused on expanding reproductive justice and women's health beyond legal abortion. By the 1990s these efforts had produced a vibrant and engaged feminist reproductive justice movement that promoted the socioeconomics of good health for all women.

Dissolved rare earth elements in the central-western sector of the Ross Sea, Southern Ocean: Geochemical tracing of seawater masses.

Science.gov (United States)

Turetta, Clara; Barbaro, Elena; Capodaglio, Gabriele; Barbante, Carlo

2017-09-01

The present essay contributes to the existing literature on rare earth elements (REEs) in the southern hemisphere by presenting the first data, to our knowledge, on the vertical profiles of dissolved REEs in 71 samples collected in the central-western sector of the Ross Sea (Southern Ocean-SO). The REEs were measured in the water samples collected during the 2002-2003 and 2005-2006 austral summers. 4 samples were collected and analysed in the framework of a test experiment, as part of the WISSARD Project (Whillans Ice Stream Subglacial Access Research Drilling). Our results show significant differences between the REE patterns of the main water masses present in the SO: we could observe specific signature in the High Salinity Shelf Water (HSSW), Ice Shelf Water (ISW) and Low Salinity Shelf Water (LSSW). A significant increase in Terbium (Tb) concentration was observed in the HSSW and ISW, the two principal water masses contributing to the formation of Antarctic Bottom Water (AABW) in the Ross Sea area, and in LSSW. Some of the HSSW samples show enrichment in Neodymium (Nd). Dissolved REE could therefore be used as tracers to understand the deep circulation of the SO (Pacific sector). We hypothesize that: (I) the characteristic dissolved REE pattern may derive from the composition of source area and from the hydrothermal activity of the central-western area of the Ross Sea; (II) the Tb anomaly observed in the AABW on the South Australian platform could be partially explained by the contribution of AABW generated in the Ross Sea region. Copyright © 2017 Elsevier Ltd. All rights reserved.

The peanut lectin-binding glycoproteins of human epidermal keratinocytes

International Nuclear Information System (INIS)

Morrison, A.I.; Keeble, S.; Watt, F.M.

1988-01-01

The peanut lectin (PNA) is known to bind more strongly to keratinocytes that are undergoing terminal differentiation than to proliferating keratinocytes. In order to investigate the significance of this change in cell-surface carbohydrate authors have identified the PNA-binding glycoproteins of cultured human keratinocytes and antibodies against them. Two heavily glycosylated bands of 110 and 250 kDa were resolved by PAGE of [ 14 C]galactose- or [ 14 C]mannose- and [ 14 C]glucosamine-labeled cell extracts eluted with galactose from PNA affinity columns. The higher molecular weight band was also detected on PNA blots of unlabeled cell extracts transferred to nitrocellulose. Both bands were sensitive to pronase digestion, but only the 250-kDa band was digested with trypsin. A rabbit antiserum that we prepared (anti-PNA-gp) immunoprecipitated both bands from cell extracts. In contrast to PNA, anti-PNA-gp bound equally to proliferating and terminally differentiating cells, indicating that some epitope(s) of the PNA-binding glycoproteins is present on the cell surface prior to terminal differentiation. When keratinocytes grown as a monolayer in low-calcium medium were switched to medium containing 2 mM calcium ions in order to induce desmosome formation and stratification, there was a dramatic redistribution of the PNA-binding glycoproteins, which became concentrated at the boundaries between cells. This may suggest a role for the glycoproteins in cell-cell interactions during stratification

A Novel Method for Detection of Glycoproteins on Sodium Dodecyl Sulphate Polyacrylamide Gel Using Radio-Iodinated Tyrosine

DEFF Research Database (Denmark)

Nalla, Amarnadh; Draz, Hossam M.; Dole, Anita

2009-01-01

The aim of this study is to develop a novel method for detection of glycoproteins on polyacrylamide gel. In this method, radio-iodinated-tyrosine (125I-tyrosine) was conjugated to glycoprotein by schiff's base mechanism on the sodium dodecyl sulfate- polyacrylamide gel. Ovalbumin and Concanavalin...... of glycoproteins using 125I-tyrosine selectively detected ovalbumin. Present results showed that MPD enhanced glycoprotein detection method can be used as a sensitive tool for the detection of glycoproteins on polyacrylamide gel...

Spatial Localization of the Ebola Virus Glycoprotein Mucin-Like Domain Determined by Cryo-Electron Tomography

OpenAIRE

Tran, Erin E. H.; Simmons, James A.; Bartesaghi, Alberto; Shoemaker, Charles J.; Nelson, Elizabeth; White, Judith M.; Subramaniam, Sriram

2014-01-01

The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.

P-glycoprotein targeted nanoscale drug carriers

KAUST Repository

Li, Wengang; Abu Samra, Dina Bashir Kamil; Merzaban, Jasmeen; Khashab, Niveen M.

2013-01-01

Multi-drug resistance (MDR) is a trend whereby tumor cells exposed to one cytotoxic agent develop cross-resistance to a range of structurally and functionally unrelated compounds. P -glycoprotein (P -gp) efflux pump is one of the mostly studied drug

Multiple genes encode the major surface glycoprotein of Pneumocystis carinii

DEFF Research Database (Denmark)

Kovacs, J A; Powell, F; Edman, J C

1993-01-01

hydrophobic region at the carboxyl terminus. The presence of multiple related msg genes encoding the major surface glycoprotein of P. carinii suggests that antigenic variation is a possible mechanism for evading host defenses. Further characterization of this family of genes should allow the development......The major surface antigen of Pneumocystis carinii, a life-threatening opportunistic pathogen in human immunodeficiency virus-infected patients, is an abundant glycoprotein that functions in host-organism interactions. A monoclonal antibody to this antigen is protective in animals, and thus...... blot studies using chromosomal or restricted DNA, the major surface glycoproteins are the products of a multicopy family of genes. The predicted protein has an M(r) of approximately 123,000, is relatively rich in cysteine residues (5.5%) that are very strongly conserved, and contains a well conserved...

Glycoproteins of axonal transport: affinity chromatography on fucose-specific lectins

Energy Technology Data Exchange (ETDEWEB)

Gustavsson, S.; Ohlson, C.; Karlsson, J.O.

1982-03-01

Rapidly transported fucose-labeled glycoproteins from axons of rabbit retinal ganglion cells were solubilized with nonionic detergents. The solubilized components were subjected to affinity chromatography on three different fucose-specific lectins. A recently characterized fucose-specific lectin from Aleuria aurantia bound reversibly approximately 60% of the applied protein-bound radioactivity. The lectins from Lotus tetragonolobus and Ulex europaeus bound are very small proportions of the labeled rapidly transported glycoproteins.

WHISPERS Project on the easternmost slope of the Ross Sea (Antarctica): preliminary results.

Science.gov (United States)

Olivo, E.; De Santis, L.; Bergamasco, A.; Colleoni, F.; Gales, J. A.; Florindo-Lopez, C.; Kim, S.; Kovacevic, V.; Rebesco, M.

2017-12-01

The advance and retreat of the West Antarctic Ice Sheet from the outer continental shelf and the oceanic circulation are the main causes of the depositional processes on the Ross Sea continental slope, at present time and during the most of the Cenozoic. Currently the Antarctic Bottom Water formation is directly linked to the relatively warm Circumpolar Deep Water that, encroaching the continental shelf, mixes with the colder Ross Sea Bottom Water. Detailed multibeam and geological surveys useful to locate and characterize peculiar morphological structures on the bottom are essential to study how the glacial and oceanographic processes interact with the seabed sediments. In the framework of the PNRA-WHISPERS project (XXXIIth Italian Antarctic expedition - January/March 2017), new multibeam bathymetric, sub-bottom chirp, were acquired from the easternmost margin of the Ross Sea, on the southeastern side of the Hayes Bank, usually covered by sea ice. We observed on the upper slope erosional features (incised gullies of likely glacial meltwater origin). A broad scar in the upper slope is characterized by an elongated SSW-NNE ridge (10 km long, 850-1200 m water depth, 2 km wide), that may be a remnants of previous glacial or debris flow deposits, eroded by meltwater outwash discharge at the beginning of grounding ice retreat and by RSBW cascading along the slope, as documented by Expandable Bathy-Thermograph and Acoustic Depth Current Profile data. Sub-bottom chirp profiles crossing this ridge show a very low amplitude reflective sea bed, supporting the hypothesis of its soft sediment nature, in good agreement with a very low acoustic velocity obtained by multichannel seismic data reprocessing. The occurrence of internal stratification on 2D multichannel seismic profiles would discount a gas-fluids related mud volcano origin. No sediment cores were collected, due to bad sea conditions and limited ship time, further data collection would be needed to fully understand

MR imaging of right ventricular function after the Ross procedure for aortic valve replacement: initial experience

NARCIS (Netherlands)

Grotenhuis, Heynric B.; de Roos, Albert; Ottenkamp, Jaap; Schoof, Paul H.; Vliegen, Hubert W.; Kroft, Lucia J. M.

2008-01-01

PURPOSE: To prospectively assess right ventricular (RV) function after the Ross procedure by using magnetic resonance (MR) imaging. MATERIALS AND METHODS: The local ethics committee approved the study and informed consent was obtained from all participants prior to enrollment in the study. Seventeen

Development of a radioimmunoassay for 'Tamm-Horsfall-like' glycoprotein in serum and cerebrospinal fluid

International Nuclear Information System (INIS)

Hartmann, L.; Bringuier, A.-F.; Schuller, E.

1983-01-01

Affinity chromatography purification was combined with a radioimmunoassay for 'Tamm-Horsfall-like' glycoprotein. This enabled serum comcentrations to be established and to demonstrate its presence in cerebrospinal fluid for the first time. This assay method used in different circumstances suggests a multifocal synthesis. Nevertheless, urinary Tamm-Horsfall glycoprotein so far must be distinguished from the serum or cerebrospinal fluid Tamm-Horsfall-like glycoprotein. (Auth.)

Dynamics of the Ross-Stirling engine

Energy Technology Data Exchange (ETDEWEB)

Doige, A G; Walker, G

1986-01-01

A computer model has been developed for the simulation of the dynamic loading in a Stirling engine having a Ross linkage as the drive mechanism. The model is based on a complete theoretical formulation of the dynamics of the system. The masses and moments of inertia of all moving components have been included in the model. The computer program can be used for determining the effect of changes in many design parameters on the geometry, velocities, accelerations, dynamic loading and reactions at all pin connections and engine-mount locations. In this paper, emphasis is given to assessing the general characteristics of the reaction forces at the pin connections and to the reduction of overall engine shaking forces by simple balancing methods. The most heavily loaded element in the engine is the pin connecting the crank and the yoke. The force at that location is a combination of a static load produced by gas pressure in the cylinders and a combined inertia load for the whole engine which increases with the square of the rotational speed. 6 refs., 12 figs., 2 tabs.

Infrared speckle observations of the binary Ross 614 AB - combined shift-and-add and zero-and-add analysis

International Nuclear Information System (INIS)

Davey, B.L.K.; Bates, R.H.T.; Cocke, W.J.; Mccarthy, D.W. Jr.; Christou, J.C.

1989-01-01

One-dimensional infrared speckle scans of Ross 614 AB were recorded at a wavelength of 2.2 microns, and the three bins corresponding to the three best seeing conditions were further processed by applying a shift-and-add algorithm to the set of images contained within each bin, generating three shift-and-add images with differing shift-and-add point-spread functions. A zero-and-add technique was used to deconvolve the three shift-and-add images in order to obtain parameters corresponding to the separation and the brightness ratio of a two-component model of Ross 614 Ab. Least-squares analysis results reveal a separation of 1.04 arcsec and a brightness ratio of 4.3 for the binary system at this wavelength. 31 refs

Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography.

Science.gov (United States)

Tran, Erin E H; Simmons, James A; Bartesaghi, Alberto; Shoemaker, Charles J; Nelson, Elizabeth; White, Judith M; Subramaniam, Sriram

2014-09-01

The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Time budgets of Snow Geese Chen caerulescens and Ross's Geese Chen rossii in mixed flocks: Implications of body size, ambient temperature and family associations

Science.gov (United States)

Jonsson, J.E.; Afton, A.D.

2009-01-01

Body size affects foraging and forage intake rates directly via energetic processes and indirectly through interactions with social status and social behaviour. Ambient temperature has a relatively greater effect on the energetics of smaller species, which also generally are more vulnerable to predator attacks than are larger species. We examined variability in an index of intake rates and an index of alertness in Lesser Snow Geese Chen caerulescens caerulescens and Ross's Geese Chen rossii wintering in southwest Louisiana. Specifically we examined variation in these response variables that could be attributed to species, age, family size and ambient temperature. We hypothesized that the smaller Ross's Geese would spend relatively more time feeding, exhibit relatively higher peck rates, spend more time alert or raise their heads up from feeding more frequently, and would respond to declining temperatures by increasing their proportion of time spent feeding. As predicted, we found that Ross's Geese spent more time feeding than did Snow Geese and had slightly higher peck rates than Snow Geese in one of two winters. Ross's Geese spent more time alert than did Snow Geese in one winter, but alert rates differed by family size, independent of species, in contrast to our prediction. In one winter, time spent foraging and walking was inversely related to average daily temperature, but both varied independently of species. Effects of age and family size on time budgets were generally independent of species and in accordance with previous studies. We conclude that body size is a key variable influencing time spent feeding in Ross's Geese, which may require a high time spent feeding at the expense of other activities. ?? 2008 The Authors.

Frequency of anti-glycoprotein Ia/IIa (anti-HPA-5b,-5a and anti-glycoprotein IIb/IIIa (anti-HPA-1a,-3a,-4a alloantibodies in multiparous women of African descent

Directory of Open Access Journals (Sweden)

Zaccheaus A Jeremiah

2010-05-01

Full Text Available Zaccheaus A Jeremiah1, Justina E Oburu2, Osaro Erhabor1, Fiekumo I Buseri1, Teddy C Adias31Haematology and Blood Transfusion Science Unit, Department of Medical Laboratory Sciences, College of Health Sciences, Niger Delta University, Wilberforce Island, Nigeria; 2Department of Haematology and Blood Transfusion, University of Port Harcourt Teaching Hospital, Port Harcourt, Nigeria; 3Rivers State University of Science and Technology, Port Harcourt, NigeriaBackground: Human platelet antibodies are often implicated in some disease conditions, such as neonatal alloimmune thrombocytopenia (NAIT, idiopathic thrombocytopenic purpura (ITP and platelet refractoriness. The frequencies of these alloantibodies have not been reported in Nigeria and West Africa.Methods: Screening for allontibodies to human platelet antigens (HPA was undertaken using the GTI PakPlus® qualitative solid phase ELISA reagent. Platelet count was done using the ICSH approved procedure using 1% ammonium oxalate reagent.Study design: A cross-section of apparently healthy adult Nigerian multiparous non-pregnant women, who were staff of a tertiary health facility in the Niger Delta, Nigeria, were screened for alloantibodies to human platelet antigens.Results: Of the one hundred (100 women screened, the prevalence of anti-glycoprotein IIb/IIIa (anti-HPA-Ia,-3a,-4a was zero percent (0%, anti-glycoprotein Ia/IIa (anti-HPA-5b accounted for 30% of results, while anti-glycoprotein Ia/IIa (anti-HPA-5a was 18%. Parity was found to exert significant influence on the development to HPA antibodies (Fisher’s Exact Test = 11.683, P < 0.05; 13.577, P < 0.01. The platelet count of the women did not appear to exert any influence on the development of the antibodies (P > 0.05.Conclusion: This study has observed a high prevalence of anti-HPA-5b in our sample population. The prevalence of alloantibodies to HPA antigens was found to associate strongly with parity. These results indicate that there is a

[A reappraisal of the works of Elisabeth Kubler-Ross].

Science.gov (United States)

Afonso, Selene Beviláqua Chaves; Minayo, Maria Cecília de Souza

2013-09-01

This article presents a reappraisal of part of the works of Elizabeth Kubler-Ross, one of the most quoted authors addressing the end of life process, mourning and dying. Her work has contributed to a clearer understanding of these issues by health professionals, families, religious and lay people who handle and/or experience mourning. She has also been the subject of controversy related to ethical issues and the scientific rigor of her work. The books analyzed in this article are: On death and dying (1969); Questions and answers on death and dying (1971); Living with death and dying (1981); On children and death(1983); On life after death (1991) and Life lessons (2000).

Glycoproteins functionalized natural and synthetic polymers for prospective biomedical applications: A review.

Science.gov (United States)

Tabasum, Shazia; Noreen, Aqdas; Kanwal, Arooj; Zuber, Mohammad; Anjum, Muhammad Naveed; Zia, Khalid Mahmood

2017-05-01

Glycoproteins have multidimensional properties such as biodegradability, biocompatibility, non-toxicity, antimicrobial and adsorption properties; therefore, they have wide range of applications. They are blended with different polymers such as chitosan, carboxymethyl cellulose (CMC), polyvinyl pyrrolidone (PVP), polycaprolactone (PCL), heparin, polystyrene fluorescent nanoparticles (PS-NPs) and carboxyl pullulan (PC) to improve their properties like thermal stability, mechanical properties, resistance to pH, chemical stability and toughness. Considering the versatile charateristics of glycoprotein based polymers, this review sheds light on synthesis and characterization of blends and composites of glycoproteins, with natural and synthetic polymers and their potential applications in biomedical field such as drug delivery system, insulin delivery, antimicrobial wound dressing uses, targeting of cancer cells, development of anticancer vaccines, development of new biopolymers, glycoproteome research, food product and detection of dengue glycoproteins. All the technical scientific issues have been addressed; highlighting the recent advancement. Copyright © 2017 Elsevier B.V. All rights reserved.

Preparation of 131I-asialo-α1-acid glycoprotein

International Nuclear Information System (INIS)

Rijk, P.P. van

1975-01-01

α 1 -Acid glycoprotein (orosomucoid) was prepared from a byproduct of the ethanol plasma fractionation by means of ion-exchange procedures. Immunoelectrophoresis suggested a high degree of purity; the purified protein contained 13.5% sialic acid and 17.8% hexose. The α 1 -acid glycoprotein was modified by removal of sialic acid with neurominidase (E.C. 3.2.1.18) followed by iodination with 131 I. The purpose of the preparation, its potential use as a pharmacon for liver-function studies in nuclear medicine, is the subject of further study

Pre- and syn-Ross orogenic granitoids at Drake Head and Kartografov Island, Oates Coast, northern Victoria Land, East Antarctica

International Nuclear Information System (INIS)

Adams, C.J.; Roland, N.W.

2002-01-01

The majority of the Oates Coast, northern Victoria Land granitoids, typified by those at Drake Head and Kartografov Island (Harald Bay), are monzogranites with lesser granodiorites and minor quartz-monzodiorite and syenogranite. All are plagioclase-K-feldspar-biotite granitoids with additional muscovite, garnet and/or hornblende, and are subalkaline and peraluminous. Berg Granite typifies the early Ordovician, Granite Harbour Instrusive (GHI) suite of the Ross Orogen at the Oates Coast. Granitoids from Kartografov Island have higher amounts of Fe+Mg+Ti and an ambiguous Rb-Sr geochronology: they could be either pre-Ross Orogeny in age, or syn-Ross Orogeny and representing a lower structural level of GHI. The Drake Head granite gneiss has a fractionated leuco-granite composition similar to Berg Granite, and is intruded by granite and granodiorite. Rb-Sr ages indicate that all are Neoproterozoic, although the granite gneiss result is probably an errorchron age, reflecting its less uniform nature (granodiorite:649 ± 30 Ma, initial ratio 0.7065 +/- 6; granite gneiss: 682 ± 140 Ma, initial ratio 0.7107 ± 50). These late Neoproterozoic granitoids provide a source for distinctive detrital zircon age components in extensive early Paleozoic turbidites of Australia-New Zealand-Antarctica. (author). 24 refs., 5 figs., 1 tab

Nipah virus infection and glycoprotein targeting in endothelial cells

Directory of Open Access Journals (Sweden)

Maisner Andrea

2010-11-01

Full Text Available Abstract Background The highly pathogenic Nipah virus (NiV causes fatal respiratory and brain infections in animals and humans. The major hallmark of the infection is a systemic endothelial infection, predominantly in the CNS. Infection of brain endothelial cells allows the virus to overcome the blood-brain-barrier (BBB and to subsequently infect the brain parenchyma. However, the mechanisms of NiV replication in endothelial cells are poorly elucidated. We have shown recently that the bipolar or basolateral expression of the NiV surface glycoproteins F and G in polarized epithelial cell layers is involved in lateral virus spread via cell-to-cell fusion and that correct sorting depends on tyrosine-dependent targeting signals in the cytoplasmic tails of the glycoproteins. Since endothelial cells share many characteristics with epithelial cells in terms of polarization and protein sorting, we wanted to elucidate the role of the NiV glycoprotein targeting signals in endothelial cells. Results As observed in vivo, NiV infection of endothelial cells induced syncytia formation. The further finding that infection increased the transendothelial permeability supports the idea of spread of infection via cell-to-cell fusion and endothelial cell damage as a mechanism to overcome the BBB. We then revealed that both glycoproteins are expressed at lateral cell junctions (bipolar, not only in NiV-infected primary endothelial cells but also upon stable expression in immortalized endothelial cells. Interestingly, mutation of tyrosines 525 and 542/543 in the cytoplasmic tail of the F protein led to an apical redistribution of the protein in endothelial cells whereas tyrosine mutations in the G protein had no effect at all. This fully contrasts the previous results in epithelial cells where tyrosine 525 in the F, and tyrosines 28/29 in the G protein were required for correct targeting. Conclusion We conclude that the NiV glycoprotein distribution is responsible for

Isolation of glycoproteins from brown algae

DEFF Research Database (Denmark)

2015-01-01

The present invention relates to a novel process for the isolation of unique anti-oxidative glycoproteins from the pH precipitated fractions of enzymatic extracts of brown algae. Two brown seaweeds viz, Fucus serratus and Fucus vesiculosus were hydrolysed by using 3 enzymes viz, Alcalase, Viscozyme...

Molecular cloning and expression of cDNA encoding a lumenal calcium binding glycoprotein from sarcoplasmic reticulum

International Nuclear Information System (INIS)

Leberer, E.; Charuk, J.H.M.; MacLennan, D.H.; Green, N.M.

1989-01-01

Antibody screening was used to isolate a cDNA encoding the 160-kDa glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum. The cDNA is identical to that encoding the 53-kDa glycoprotein except that it contains an in-frame insertion of 1,308 nucleotides near its 5' end, apparently resulting from alternative splicing. The protein encoded by the cDNA would contain a 19-residue NH 2 -terminal signal sequence and a 453-residue COOH-terminal sequence identical to the 53-kDa glycoprotein. It would also contain a 436-amino acid insert between these sequences. This insert would be highly acidic, suggesting that it might bind Ca 2+ . The purified 160-kDa glycoprotein and the glycoprotein expressed in COS-1 cells transfected with cDNA encoding the 160-kDa glycoprotein were shown to bind 45 C 2+ in a gel overlay assay. The protein was shown to be located in the lumen of the sarcoplasmic reticulum and to be associated through Ca 2+ with the membrane. The authors propose that this lumenal Ca 2+ binding glycoprotein of the sarcoplasmic reticulum be designated sarcalumenin

Australine, a pyrrolizidine alkaloid that inhibits amyloglucosidase and glycoprotein processing

International Nuclear Information System (INIS)

Tropea, J.E.; Molyneux, R.J.; Kaushal, G.P.; Pan, Y.T.; Mitchell, M.; Elbein, A.D.

1989-01-01

Australine is a polyhydroxylated pyrrolizidine alkaloid that was isolated from the seeds of the Australian tree Castanospermum australe and characterized by NMR and X-ray diffraction analysis. Since swainsonine and catanospermine are polyhydroxylated indolizidine alkaloids that inhibit specific glycosidases, the authors tested australine against a variety of exoglycosidases to determine whether it would inhibit any of these enzymes. This alkaloid proved to be a good inhibitor of the α-glucosidase amyloglucosidase (50% inhibition at 5.8 μM), but it did not inhibit β-glucosidase, α- or β-mannosidase, or α- or β-galactosidase. The inhibition of amyloglucosidase was of a competitive nature. Australine also inhibited the glycoprotein processing enzyme glucosidase I, but had only slight activity toward glucosidase II. When incubated with cultured cells, this alkaloid inhibited glycoprotein processing at the glucosidase I step and caused the accumulation of glycoproteins with Glc 3 Man 7-9 (GlcNAc) 2 -oligosaccharides

Statins Suppress Ebola Virus Infectivity by Interfering with Glycoprotein Processing.

Science.gov (United States)

Shrivastava-Ranjan, Punya; Flint, Mike; Bergeron, Éric; McElroy, Anita K; Chatterjee, Payel; Albariño, César G; Nichol, Stuart T; Spiropoulou, Christina F

2018-05-01

Ebola virus (EBOV) infection is a major public health concern due to high fatality rates and limited effective treatments. Statins, widely used cholesterol-lowering drugs, have pleiotropic mechanisms of action and were suggested as potential adjunct therapy for Ebola virus disease (EVD) during the 2013-2016 outbreak in West Africa. Here, we evaluated the antiviral effects of statin (lovastatin) on EBOV infection in vitro Statin treatment decreased infectious EBOV production in primary human monocyte-derived macrophages and in the hepatic cell line Huh7. Statin treatment did not interfere with viral entry, but the viral particles released from treated cells showed reduced infectivity due to inhibition of viral glycoprotein processing, as evidenced by decreased ratios of the mature glycoprotein form to precursor form. Statin-induced inhibition of infectious virus production and glycoprotein processing was reversed by exogenous mevalonate, the rate-limiting product of the cholesterol biosynthesis pathway, but not by low-density lipoprotein. Finally, statin-treated cells produced EBOV particles devoid of the surface glycoproteins required for virus infectivity. Our findings demonstrate that statin treatment inhibits EBOV infection and suggest that the efficacy of statin treatment should be evaluated in appropriate animal models of EVD. IMPORTANCE Treatments targeting Ebola virus disease (EVD) are experimental, expensive, and scarce. Statins are inexpensive generic drugs that have been used for many years for the treatment of hypercholesterolemia and have a favorable safety profile. Here, we show the antiviral effects of statins on infectious Ebola virus (EBOV) production. Our study reveals a novel molecular mechanism in which statin regulates EBOV particle infectivity by preventing glycoprotein processing and incorporation into virus particles. Additionally, statins have anti-inflammatory and immunomodulatory effects. Since inflammation and dysregulation of the immune

Death age, seasonality, taphonomy and colonization of seal carcasses from Ulu Peninsula, James Ross Island, Antarctic Peninsula

Czech Academy of Sciences Publication Activity Database

Nývlt, D.; Nývltová Fišáková, Miriam; Barták, M.; Stachoň, Z.; Pavel, V.; Mlčoch, B.; Láska, K.

2016-01-01

Roč. 28, č. 1 (2016), s. 3-16 ISSN 0954-1020 Institutional support: RVO:68081758 Keywords : James Ross Island * preservation state * Prince Gustav Channel * sea ice * seal behaviour Subject RIV: EH - Ecology, Behaviour Impact factor: 1.461, year: 2016

Bacterivory by a Summer Assemblage of Nanoplankton in the Ross Sea, Antarctica: Mixotrophic Versus Heterotrophic Protists

Science.gov (United States)

Sanders, R. W.; Gast, R. J.

2016-02-01

Many protists traditionally described as phototrophic have recently been shown to have retained the primitive trait of phagotrophy, and thus function as mixotrophs. Mixotrophic nanoflagellates were identified in every sample examined from a summer cruise in the Ross Sea, Antarctica, where they often were more abundant than heterotrophic nanoflagellates that have previously been considered the major bacterivores in marine waters. Mixotrophs, identified by uptake of fluorescent tracers, comprised similar proportions (9-75%) of the total bacterivorous flagellates in summer as were previously determined for an earlier spring cruise in the Ross Sea. Protist diversity also was linked to functional bacterivores using a culture-independent method in which BrdU-labeled DNA of bacterial prey was incorporated into the DNA of eukaryotic grazers. Immunoprecipitation of the BrdU-labeld DNA was followed by high-throughput sequencing to identify a diverse group of bacterivores, including numerous uncultured eukaryotes. However, its utility for identification of mixotrophs was limited by the availability of sequences from known mixotrophs.

The expression and serological reactivity of recombinant canine herpesvirus 1 glycoprotein D

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MarkéŽta Vaňkov‡á

2016-01-01

Full Text Available The aim of this work was to express recombinant glycoprotein D of canine herpesvirus 1 in bacterial cells and to evaluate its diagnostic sensitivity and specificity when compared to traditional serological methods. The gene fragment coding glycoprotein D of canine herpesvirus 1 was amplified by polymerase chain reaction, cloned into plasmid vector and expressed in Escherichia coli cells. Recombinant protein was then purified and used as an antigen in immunoblot for a detection of canine herpesvirus 1 specific antibodies. Antibody testing was performed on the panel of 100 canine sera by immunoblot with recombinant glycoprotein D as antigen and compared with indirect immunofluorescence assay. Serum samples were collected from 83 dogs with no history of canine herpesvirus 1 or reproductive disorders, and from 17 dogs from breeding kennels with a history of canine herpesvirus 1 related reproductive disorders. Sensitivity of glycoprotein D based immunoblot was 89.2% and specificity was 93%. Kappa value was calculated to be 0.8 between immunoblot and indirect immunofluorescence assay. Antibodies against canine herpesvirus 1 infection were detected in 33% of samples by immunoblot assay. Our study confirms that recombinant glycoprotein D expressed in bacterial cells could be used as a suitable and sensitive antigen for immunological tests and that herpesvirus infection seems to be common among the canine population in the Czech Republic.

Early Winter Sea Ice Dynamics in the Ross Sea from In Situ and Satellite Observations

Science.gov (United States)

Maksym, T.; Ackley, S. F.; Stammerjohn, S. E.; Tison, J. L.; Hoeppner, K.

2017-12-01

The Ross Sea sea ice cover is one of the few regions of the cryosphere that have been expanding in recent decades. However, 2017 saw a significantly delayed autumn ice advance and record low early winter sea ice extent. Understanding the causes and impacts of this variability has been hampered by a lack of in situ observations. A winter cruise into the Ross Sea in April-June 2017 provided some of the only in situ winter observations of sea ice processes in this region in almost 20 years. We present a first look at data from arrays of drifting buoys deployed in the ice pack and outflow from these polynyas, supplemented by a suite of high-resolution synthetic aperture radar (SAR) data. Additional observations included high-resolution sonar imagery of ice deformation features from an autonomous underwater vehicle, shipboard visual observations of sea ice properties, and in situ measurements of snow and thickness and structural properties. These data show that the delay in ice advance led to a thin, highly dynamic sea ice pack, with substantial ice production and export from the Ross Ice Shelf and Terra Nova Bay polynyas. Despite these high rates of ice production, the pack ice remained thin due to rapid export and northward drift. Compared to the only prior winter observations made in 1995 and 1998, the ice was thinner, with less ridging and snow cover, reflecting a younger ice cover. Granular ice was less prevalent than in these prior cruises, particularly in the outer pack, likely due to less snow ice formation and less pancake ice formation at the advancing ice edge. Despite rapid basal ice growth, the buoy data suggest that deformation may be the dominant mechanism for sea ice thickening in the pack once an initial ice cover forms.

Global site-specific analysis of glycoprotein N-glycan processing.

Science.gov (United States)

Cao, Liwei; Diedrich, Jolene K; Ma, Yuanhui; Wang, Nianshuang; Pauthner, Matthias; Park, Sung-Kyu Robin; Delahunty, Claire M; McLellan, Jason S; Burton, Dennis R; Yates, John R; Paulson, James C

2018-06-01

N-glycans contribute to the folding, stability and functions of the proteins they decorate. They are produced by transfer of the glycan precursor to the sequon Asn-X-Thr/Ser, followed by enzymatic trimming to a high-mannose-type core and sequential addition of monosaccharides to generate complex-type and hybrid glycans. This process, mediated by the concerted action of multiple enzymes, produces a mixture of related glycoforms at each glycosite, making analysis of glycosylation difficult. To address this analytical challenge, we developed a robust semiquantitative mass spectrometry (MS)-based method that determines the degree of glycan occupancy at each glycosite and the proportion of N-glycans processed from high-mannose type to complex type. It is applicable to virtually any glycoprotein, and a complete analysis can be conducted with 30 μg of protein. Here, we provide a detailed description of the method that includes procedures for (i) proteolytic digestion of glycoprotein(s) with specific and nonspecific proteases; (ii) denaturation of proteases by heating; (iii) sequential treatment of the glycopeptide mixture with two endoglycosidases, Endo H and PNGase F, to create unique mass signatures for the three glycosylation states; (iv) LC-MS/MS analysis; and (v) data analysis for identification and quantitation of peptides for the three glycosylation states. Full coverage of site-specific glycosylation of glycoproteins is achieved, with up to thousands of high-confidence spectra hits for each glycosite. The protocol can be performed by an experienced technician or student/postdoc with basic skills for proteomics experiments and takes ∼7 d to complete.

Analytical Pipeline for Discovery and Verification of Glycoproteins from Plasma-Derived Extracellular Vesicles as Breast Cancer Biomarkers.

Science.gov (United States)

Chen, I-Hsuan; Aguilar, Hillary Andaluz; Paez Paez, J Sebastian; Wu, Xiaofeng; Pan, Li; Wendt, Michael K; Iliuk, Anton B; Zhang, Ying; Tao, W Andy

2018-05-15

Glycoproteins comprise more than half of current FDA-approved protein cancer markers, but the development of new glycoproteins as disease biomarkers has been stagnant. Here we present a pipeline to develop glycoproteins from extracellular vesicles (EVs) through integrating quantitative glycoproteomics with a novel reverse phase glycoprotein array and then apply it to identify novel biomarkers for breast cancer. EV glycoproteomics show promise in circumventing the problems plaguing current serum/plasma glycoproteomics and allowed us to identify hundreds of glycoproteins that have not been identified in blood. We identified 1,453 unique glycopeptides representing 556 glycoproteins in EVs, among which 20 were verified significantly higher in individual breast cancer patients. We further applied a novel glyco-specific reverse phase protein array to quantify a subset of the candidates. Together, this study demonstrates the great potential of this integrated pipeline for biomarker discovery.

Homologous and heterologous antibody responses of mice immunized with purified feline herpesvirus type 1 and canine herpesvirus glycoproteins.

Science.gov (United States)

Limcumpao, J A; Horimoto, T; Xuan, X N; Tohya, Y; Azetaka, M; Takahashi, E; Mikami, T

1991-06-01

The three glycoproteins each of feline herpesvirus type 1 (FHV-1) and canine herpesvirus (CHV) were purified by affinity chromatography using glycoprotein-specific monoclonal antibodies and used individually or in combination in immunizing mice to determine their relative immunogenicity. All the glycoproteins induced detectable virus neutralizing antibodies to the homologous virus but FHV-1 gp143/108 and its cross-reacting counterpart, CHV gp145/112, elicited the highest titers not only to the homologous virus but to the heterologous virus as well. The production of ELISA antibodies after glycoprotein immunization was variable, while hemagglutination-inhibiting antibodies were produced by only 1 out of 10 FHV-1 gp60-inoculated mice. In general, the antibody titers induced by CHV glycoproteins were lower than those by FHV-1 glycoproteins. These results indicate that these glycoproteins may be useful as subunit vaccines against FHV-1 and CHV infections.

Ross Works on the Assembly Concept for Construction of Erectable Space Structure (ACCESS) During

Science.gov (United States)

1985-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia, and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo, astronaut Ross works on ACCESS high above the orbiter. The primary objective of these experiments was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

The Xanthophyceae and Chlorophyceae of the Western Ross Sea, Victoria Land, Antarctica and Macquarie Island collected under the direction of Prof. Dr. J. S. Zaneveld (1963—1967)

NARCIS (Netherlands)

Wagner, H.P.; Zaneveld, J.S.

1988-01-01

During the austral summer seasons of 1963-1964 and 1964-1965, and during the austral winter season of 1967 Prof. Dr. J.S. Zaneveld made collection expeditions to the western Ross Sea. In 1965 collections were also made from the U.S.C.G. icebreaker ‘Glacier’, around the western Ross Sea and around

Understanding Ice Shelf Basal Melting Using Convergent ICEPOD Data Sets: ROSETTA-Ice Study of Ross Ice Shelf

Science.gov (United States)

Bell, R. E.; Frearson, N.; Tinto, K. J.; Das, I.; Fricker, H. A.; Siddoway, C. S.; Padman, L.

2017-12-01

The future stability of the ice shelves surrounding Antarctica will be susceptible to increases in both surface and basal melt as the atmosphere and ocean warm. The ROSETTA-Ice program is targeted at using the ICEPOD airborne technology to produce new constraints on Ross Ice Shelf, the underlying ocean, bathymetry, and geologic setting, using radar sounding, gravimetry and laser altimetry. This convergent approach to studying the ice-shelf and basal processes enables us to develop an understanding of the fundamental controls on ice-shelf evolution. This work leverages the stratigraphy of the ice shelf, which is detected as individual reflectors by the shallow-ice radar and is often associated with surface scour, form close to the grounding line or pinning points on the ice shelf. Surface accumulation on the ice shelf buries these reflectors as the ice flows towards the calving front. This distinctive stratigraphy can be traced across the ice shelf for the major East Antarctic outlet glaciers and West Antarctic ice streams. Changes in the ice thickness below these reflectors are a result of strain and basal melting and freezing. Correcting the estimated thickness changes for strain using RIGGS strain measurements, we can develop decadal-resolution flowline distributions of basal melt. Close to East Antarctica elevated melt-rates (>1 m/yr) are found 60-100 km from the calving front. On the West Antarctic side high melt rates primarily develop within 10 km of the calving front. The East Antarctic side of Ross Ice Shelf is dominated by melt driven by saline water masses that develop in Ross Sea polynyas, while the melting on the West Antarctic side next to Hayes Bank is associated with modified Continental Deep Water transported along the continental shelf. The two sides of Ross Ice Shelf experience differing basal melt in part due to the duality in the underlying geologic structure: the East Antarctic side consists of relatively dense crust, with low amplitude

The Kubler-Ross model, physician distress, and performance reporting.

Science.gov (United States)

Smaldone, Marc C; Uzzo, Robert G

2013-07-01

Physician performance reporting has been proposed as an essential component of health-care reform, with the aim of improving quality by providing transparency and accountability. Despite strong evidence demonstrating regional variation in practice patterns and lack of evidence-based care, public outcomes reporting has been met with resistance from medical professionals. Application of the Kubler-Ross 'five stages of grief' model--a conceptual framework consisting of a series of emotional stages (denial, anger, bargaining, depression, and acceptance) inspired by work with terminally ill patients--could provide some insight into why physicians are reluctant to accept emerging quality-reporting mechanisms. Physician-led quality-improvement initiatives are vital to contemporary health-care reform efforts and applications in urology, as well as other medical disciplines, are currently being explored.

Ansel Adams's Eucalyptus Tree, Fort Ross: Nature, Photography, and the Search for California

OpenAIRE

Adam Arenson

2005-01-01

This article considers the image of California evoked in the unusual Ansel Adams photograph Eucalyptus Tree, Fort Ross, California (1969), a Polaroid Land image of the garrison fence and an aged eucalyptus tree. Considering the participation of Russian occupation, Australian cross-pollination, Carleton Watkins's early photographs of redwoods, automotive and tourist images in the creation of this distinctive California place, the article argues that to understand Ansel Adams's work, we must no...

Bioactivity of proteins isolated from Lactobacillus plantarum L67 treated with Zanthoxylum piperitum DC glycoprotein.

Science.gov (United States)

Song, S; Oh, S; Lim, K-T

2015-06-01

Lactobacilli in the human gastrointestinal tract have beneficial effects on the health of their host. To enhance these effects, the bioactivity of lactobacilli can be fortified through exogenous dietary or pharmacological agents, such as glycoproteins. To elucidate the inductive effect of Zanthoxylum piperitum DC (ZPDC) glycoprotein on Lactobacillus plantarum L67, we evaluated the radical-scavenging activity, anti-oxidative enzymes (SOD, GPx and CAT), growth rate, ATPase activity and β-galactosidase activity of this strain. When Lact. plantarum L67 was treated with ZPDC glycoprotein at different concentrations, the intensities of a few SDS-PAGE bands were slightly changed. The amount of a 23 kDa protein was increased upon treatment with increasing concentrations of ZPDC glycoprotein. The results of this study indicate that the radical-scavenging activity for O2(-) and OH¯, but not for the DPPH radical, increased in a concentration-dependent manner after treatment with ZPDC glycoprotein. The activation of anti-oxidative enzymes (SOD, GPx and CAT), growth rate and β-galactosidase activity also increased in a concentration-dependent manner in response to ZPDC glycoprotein treatment, whereas ATPase activity was decreased. In summary, ZPDC glycoprotein stimulated an increase in the bioactivity of Lact. plantarum L67. Significance and impact of the study: This study demonstrated that Lactobacillus plantarum L67 possesses anti-oxidative activity. This strain of lactic bacteria has been known to have various probiotic uses, such as yogurt starters and dietary additional supplements. We found, through this experiment, that the protein has a strong anti-oxidative character, and the activity can be enhanced by treatment with Zanthoxylum piperitum DC (ZPDC) glycoprotein. This study may be application of Lact. plantarum L67 treated by ZPDC glycoprotein in yogurt fermentation. It could be one of the avenues of minimizing yogurt postacidification during storage. In addition

Colonial Fantasies, Narrative Borders, and the Canadian North in the Works of Germany's Colin Ross(1885-1945

Directory of Open Access Journals (Sweden)

Nicole Pissowotzki

2009-03-01

Full Text Available This paper argues that the Canadian North is a discursive construction, within which German colonial fantasies emerge. In particular, I argue that it is through bordering that colonial fantasies of German Lebensraum ("living space" in the Canadian North are brought into being. I further argue that the German biologist and geographer Friedrich Ratzel (1844-1904, with his view of the "organic state," provides the ideological framework for colonial fantasies in the travel writings of Colin Ross. I focus on the writer's colonial imagination and his perception of borders, and on how both relate to the Canadian North. I show that seemingly bare geographical information and demographical data, provided in Ross' travelogues, carry colonial fantasies of German spaces in the Canadian North. Those spaces are bordered by "shared histories" and "narrative boundaries," thus constructing a collective German colonial identity (cf. Eder 2006, 255-257.

The haemagglutination activity of equine herpesvirus type 1 glycoprotein C.

Science.gov (United States)

Andoh, Kiyohiko; Hattori, Shiho; Mahmoud, Hassan Y A H; Takasugi, Maaya; Shimoda, Hiroshi; Bannai, Hiroshi; Tsujimura, Koji; Matsumura, Tomio; Kondo, Takashi; Kirisawa, Rikio; Mochizuki, Masami; Maeda, Ken

2015-01-02

Equine herpesvirus type 1 (EHV-1) has haemagglutination (HA) activity toward equine red blood cells (RBCs), but the identity of its haemagglutinin is unknown. To identify the haemagglutinin of EHV-1, the major glycoproteins of EHV-1 were expressed in 293T cells, and the cells or cell lysates were mixed with equine RBCs. The results showed that only EHV-1 glycoprotein C (gC)-producing cells adsorbed equine RBCs, and that the lysate of EHV-1 gC-expressing cells agglutinated equine RBCs. EHV-1 lacking gC did not show HA activity. HA activity was inhibited by monoclonal antibodies (MAbs) specific for gC, but not by antibodies directed against other glycoproteins. In addition, HA activity was not inhibited by the addition of heparin. These results indicate that EHV-1 gC can bind equine RBCs irrespective of heparin, in contrast to other herpesvirus gC proteins. Copyright © 2014 Elsevier B.V. All rights reserved.

Australine, a pyrrolizidine alkaloid that inhibits amyloglucosidase and glycoprotein processing

Energy Technology Data Exchange (ETDEWEB)

Tropea, J.E.; Molyneux, R.J.; Kaushal, G.P.; Pan, Y.T.; Mitchell, M.; Elbein, A.D. (Univ. of Texas Health Science Center, San Antonio (USA))

1989-03-07

Australine is a polyhydroxylated pyrrolizidine alkaloid that was isolated from the seeds of the Australian tree Castanospermum australe and characterized by NMR and X-ray diffraction analysis. Since swainsonine and catanospermine are polyhydroxylated indolizidine alkaloids that inhibit specific glycosidases, the authors tested australine against a variety of exoglycosidases to determine whether it would inhibit any of these enzymes. This alkaloid proved to be a good inhibitor of the {alpha}-glucosidase amyloglucosidase (50% inhibition at 5.8 {mu}M), but it did not inhibit {beta}-glucosidase, {alpha}- or {beta}-mannosidase, or {alpha}- or {beta}-galactosidase. The inhibition of amyloglucosidase was of a competitive nature. Australine also inhibited the glycoprotein processing enzyme glucosidase I, but had only slight activity toward glucosidase II. When incubated with cultured cells, this alkaloid inhibited glycoprotein processing at the glucosidase I step and caused the accumulation of glycoproteins with Glc{sub 3}Man{sub 7-9}(GlcNAc){sub 2}-oligosaccharides.

Western Ross Sea continental slope gravity currents

Science.gov (United States)

Gordon, Arnold L.; Orsi, Alejandro H.; Muench, Robin; Huber, Bruce A.; Zambianchi, Enrico; Visbeck, Martin

2009-06-01

Antarctic Bottom Water of the world ocean is derived from dense Shelf Water that is carried downslope by gravity currents at specific sites along the Antarctic margins. Data gathered by the AnSlope and CLIMA programs reveal the presence of energetic gravity currents that are formed over the western continental slope of the Ross Sea when High Salinity Shelf Water exits the shelf through Drygalski Trough. Joides Trough, immediately to the east, offers an additional escape route for less saline Shelf Water, while the Glomar Challenger Trough still farther east is a major pathway for export of the once supercooled low-salinity Ice Shelf Water that forms under the Ross Ice Shelf. The Drygalski Trough gravity currents increase in thickness from ˜100 to ˜400 m on proceeding downslope from ˜600 m (the shelf break) to 1200 m (upper slope) sea floor depth, while turning sharply to the west in response to the Coriolis force during their descent. The mean current pathway trends ˜35° downslope from isobaths. Benthic-layer current and thickness are correlated with the bottom water salinity, which exerts the primary control over the benthic-layer density. A 1-year time series of bottom-water current and hydrographic properties obtained on the slope near the 1000 m isobath indicates episodic pulses of Shelf Water export through Drygalski Trough. These cold (34.75) pulses correlate with strong downslope bottom flow. Extreme examples occurred during austral summer/fall 2003, comprising concentrated High Salinity Shelf Water (-1.9 °C; 34.79) and approaching 1.5 m s -1 at descent angles as large as ˜60° relative to the isobaths. Such events were most common during November-May, consistent with a northward shift in position of the dense Shelf Water during austral summer. The coldest, saltiest bottom water was measured from mid-April to mid-May 2003. The summer/fall export of High Salinity Shelf Water observed in 2004 was less than that seen in 2003. This difference, if real

Assimilating bio-optical glider data during a phytoplankton bloom in the southern Ross Sea

Science.gov (United States)

Kaufman, Daniel E.; Friedrichs, Marjorie A. M.; Hemmings, John C. P.; Smith, Walker O., Jr.

2018-01-01

The Ross Sea is a region characterized by high primary productivity in comparison to other Antarctic coastal regions, and its productivity is marked by considerable variability both spatially (1-50 km) and temporally (days to weeks). This variability presents a challenge for inferring phytoplankton dynamics from observations that are limited in time or space, which is often the case due to logistical limitations of sampling. To better understand the spatiotemporal variability in Ross Sea phytoplankton dynamics and to determine how restricted sampling may skew dynamical interpretations, high-resolution bio-optical glider measurements were assimilated into a one-dimensional biogeochemical model adapted for the Ross Sea. The assimilation of data from the entire glider track using the micro-genetic and local search algorithms in the Marine Model Optimization Testbed improves the model-data fit by ˜ 50 %, generating rates of integrated primary production of 104 g C m-2 yr-1 and export at 200 m of 27 g C m-2 yr-1. Assimilating glider data from three different latitudinal bands and three different longitudinal bands results in minimal changes to the simulations, improves the model-data fit with respect to unassimilated data by ˜ 35 %, and confirms that analyzing these glider observations as a time series via a one-dimensional model is reasonable on these scales. Whereas assimilating the full glider data set produces well-constrained simulations, assimilating subsampled glider data at a frequency consistent with cruise-based sampling results in a wide range of primary production and export estimates. These estimates depend strongly on the timing of the assimilated observations, due to the presence of high mesoscale variability in this region. Assimilating surface glider data subsampled at a frequency consistent with available satellite-derived data results in 40 % lower carbon export, primarily resulting from optimized rates generating more slowly sinking diatoms. This

The effect of P-glycoprotein on methadone hydrochloride flux in equine intestinal mucosa.

Science.gov (United States)

Linardi, R L; Stokes, A M; Andrews, F M

2013-02-01

Methadone is an effective analgesic opioid that may have a place for the treatment of pain in horses. However, its absorption seems to be impaired by the presence of a transmembrane protein, P-glycoprotein, present in different tissues including the small intestine in other species. This study aims to determine the effect of the P-glycoprotein on methadone flux in the equine intestinal mucosa, as an indicator of in vivo drug absorption. Jejunum tissues from five horses were placed into the Ussing chambers and exposed to methadone solution in the presence or absence of Rhodamine 123 or verapamil. Electrical measurements demonstrated tissue viability for 120 min, and the flux of methadone across the jejunal membrane (mucosal to submucosal direction) was calculated based on the relative drug concentration measured by ELISA. The flux of methadone was significantly higher only in the presence of verapamil. P-glycoprotein was immunolocalized in the apical membrane of the jejunal epithelial cells (enterocytes), mainly located in the tip of the villi compared to cells of the crypts. P-glycoprotein is present in the equine jejunum and may possibly mediate the intestinal transport of methadone. This study suggests that P-glycoprotein may play a role in the poor intestinal absorption of methadone in vivo. © 2012 Blackwell Publishing Ltd.

[Pregnancy-specific beta-glycoprotein in the serum of women with a complicated early pregnancy].

Science.gov (United States)

Radikov, N

1989-01-01

The author determined pregnancy specific beta 1-glycoprotein in 109 women with threatened early pregnancy as 32 of the women suffered from abortus imminens with several unsuccessful pregnancies in the past as well as 67 women with abortus incipiens with bleeding ex utero. The author established that 87% of women with abortus imminens and preserved pregnancies had values of beta 1-glycoprotein close to those of normal pregnancy for the respective gestational week. 93% of women with abortus incipiens preserved pregnancies till term, but the specific glycoprotein was with in normal ranges. Spontaneous abortion occurred in 7% of women with low values under the 10th percentile. The present study show that examination of pregnancy specific beta 1-glycoprotein in women with threatened early pregnancy is of prognostic significance for the outcome of pregnancy.

Study on the extraction and purification of glycoprotein from the yellow seahorse, Hippocampus kuda Bleeker

Science.gov (United States)

Su, Yuting; Xu, Yongjian

2015-01-01

The optimum parameters of extraction for glycoprotein from seahorse were examined and determined by Box-Behnken combined with ultrasonic extraction technology. Column chromatography of glycoprotein was used for further purification. The optimal extraction conditions of seahorse glycoprotein were extracting time 4.3 h, salt concentration 0.08 mol/L, extracting temperature 73°C, raw material, and water ratio 1:6. At the optimal conditions, the yield of saccharide reached to 1.123%, and the yield of protein reached to 5.898%. For purifying the crude glycoprotein, the stage renounces of DEAE-52 column chromatography were done, respectively, with 0.05, 0.1, 0.5 mol/L NaHCO3 solution, and further purification was done with Sephadex G-100 column chromatography. Finally, two pieces of seahorse glycoprotein were obtained by the column chromatography, that is, HG-11 and HG-21. The saccharide content was 56.7975% and 39.479%, the protein content was 30.5475% and 51.747%, respectively. PMID:26288722

Improved radioimmunoassay for urinary Tamm-Horsfall glycoprotein. Investigation and resolution of factors affecting its quantification

Energy Technology Data Exchange (ETDEWEB)

Dawney, A B.St.J.; Thornley, C; Cattell, W R [Saint Bartholomew' s Hospital, London (UK)

1982-09-15

A rapid specific radioimmunoassay has been used to measure Tamm-Horsfall glycoprotein (TH glycoprotein) in urine, and the method described. The apparent concentration increased with increasing dilution of urine in water, reaching a plateau at 1 in 20. This increase was greater the higher the osmolality and TH glycoprotein concentration and the lower the pH of the original sample. The apparent concentration of TH glycoprotein in neat or diluted urine was not affected by freezing or by storage at 4/sup 0/C or room temperature for at least 2 days. A physiological range for the urinary excretion rate was established as 22-56 mg/24h, (considerably higher than the amount present in serum) based on samples from 29 individuals with normal renal function, as defined by their creatinine clearance. There was no significant correlation between serum concentrations of TH glycoprotein and its urinary excretion rate, nor between urinary excretion rate and creatinine clearance.

A novel PET imaging protocol identifies seizure-induced regional overactivity of P-glycoprotein at the blood-brain barrier

Science.gov (United States)

Bankstahl, Jens P.; Bankstahl, Marion; Kuntner, Claudia; Stanek, Johann; Wanek, Thomas; Meier, Martin; Ding, Xiao-Qi; Müller, Markus; Langer, Oliver; Löscher, Wolfgang

2013-01-01

About one third of epilepsy patients are pharmacoresistant. Overexpression of P-glycoprotein and other multidrug transporters at the blood-brain barrier is thought to play an important role in drug-refractory epilepsy. Thus, quantification of regionally different P-glycoprotein activity in the brain in vivo is essential to identify P-glycoprotein overactivity as the relevant mechanism for drug-resistance in an individual patient. Using the radiolabeled P-glycoprotein substrate (R)-[11C]verapamil and different doses of co-administered tariquidar, which is an inhibitor of P-glycoprotein, we evaluated whether small-animal positron emission tomography (PET) can quantify regional changes in transporter function in the rat brain at baseline and 48 h after a pilocarpine-induced status epilepticus. P-glycoprotein expression was additionally quantified by immunohistochemistry. To reveal putative seizure-induced changes in blood-brain barrier integrity, we performed gadolinium-enhanced magnetic resonance scans on a 7.0 Tesla small-animal scanner. Before P-glycoprotein modulation, brain uptake of (R)-[11C]verapamil was low in all regions investigated in control and post-status epilepticus rats. After administration of 3 mg/kg tariquidar, which inhibits P-glycoprotein only partially, we observed increased regional differentiation in brain activity uptake in post-status epilepticus versus control rats, which diminished after maximal P-glycoprotein inhibition. Regional increases in the efflux rate constant k2, but not in distribution volume VT or influx rate constant K1, correlated significantly with increases in P-glycoprotein expression measured by immunohistochemistry. This imaging protocol proves to be suitable to detect seizure-induced regional changes in P-glycoprotein activity and is readily applicable to humans, with the aim to detect relevant mechanisms of pharmacoresistance in epilepsy in vivo. PMID:21677164

Fbs1 protects the malfolded glycoproteins from the attack of peptide:N-glycanase

International Nuclear Information System (INIS)

Yamaguchi, Yoshiki; Hirao, Takeshi; Sakata, Eri; Kamiya, Yukiko; Kurimoto, Eiji; Yoshida, Yukiko; Suzuki, Tadashi; Tanaka, Keiji; Kato, Koichi

2007-01-01

Fbs1 is a cytosolic lectin putatively operating as a chaperone as well as a substrate-recognition subunit of the SCF Fbs1 ubiquitin ligase complex. To provide structural and functional basis of preferential binding of Fbs1 to unfolded glycoproteins, we herein characterize the interaction of Fbs1 with a heptapeptide carrying Man 3 GlcNAc 2 by nuclear magnetic resonance (NMR) spectroscopy and other biochemical methods. Inspection of the NMR data obtained by use of the isotopically labeled glycopeptide indicated that Fbs1 interacts with sugar-peptide junctions, which are shielded in native glycoprotein, in many cases, but become accessible to Fbs1 in unfolded glycoproteins. Furthermore, Fbs1 was shown to inhibit deglycosylation of denatured ribonuclease B by a cytosolic peptide:N-glycanase (PNGase). On the basis of these data, we suggest that Fbs1 captures malfolded glycoproteins, protecting them from the attack of PNGase, during the chaperoning or ubiquitinating operation in the cytosol

Purification and characterization of a soluble glycoprotein from garlic (Allium sativum) and its in vitro bioactivity.

Science.gov (United States)

Wang, Yan; Zou, Tingting; Xiang, Minghui; Jin, Chenzhong; Zhang, Xuejiao; Chen, Yong; Jiang, Qiuqing; Hu, Yihong

2016-10-02

A soluble glycoprotein was purified to homogeneity from ripe garlic (Allium sativum) bulbs using ammonium sulfate precipitation, Sephadex G-100 gel filtration, and diethylaminoethyl-52 cellulose anion-exchange chromatography. A native mass of 55.7 kDa estimated on gel permeation chromatography and a molecular weight of 13.2 kDa observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis supported that the glycoprotein is a homotetramer. β-Elimination reaction result suggested that the glycoprotein is an N-linked type. Fourier-transform infrared spectroscopy proved that it contains sugar. Gas chromatography-mass spectrometer analysis showed that its sugar component was galactose. The glycoprotein has 1,1-diphenyl-2-picrylhydrazil free radical scavenging activity and the peroxidation inhibition ability to polyunsaturated fatty acid. These results indicated that the glycoprotein has potential for food additives, functional foods, and even biotechnological and medical applications.

Annotating Human P-Glycoprotein Bioassay Data.

Science.gov (United States)

Zdrazil, Barbara; Pinto, Marta; Vasanthanathan, Poongavanam; Williams, Antony J; Balderud, Linda Zander; Engkvist, Ola; Chichester, Christine; Hersey, Anne; Overington, John P; Ecker, Gerhard F

2012-08-01

Huge amounts of small compound bioactivity data have been entering the public domain as a consequence of open innovation initiatives. It is now the time to carefully analyse existing bioassay data and give it a systematic structure. Our study aims to annotate prominent in vitro assays used for the determination of bioactivities of human P-glycoprotein inhibitors and substrates as they are represented in the ChEMBL and TP-search open source databases. Furthermore, the ability of data, determined in different assays, to be combined with each other is explored. As a result of this study, it is suggested that for inhibitors of human P-glycoprotein it is possible to combine data coming from the same assay type, if the cell lines used are also identical and the fluorescent or radiolabeled substrate have overlapping binding sites. In addition, it demonstrates that there is a need for larger chemical diverse datasets that have been measured in a panel of different assays. This would certainly alleviate the search for other inter-correlations between bioactivity data yielded by different assay setups.

Chemoenzymatic site-specific labeling of influenza glycoproteins as a tool to observe virus budding in real time.

Directory of Open Access Journals (Sweden)

Maximilian Wei-Lin Popp

Full Text Available The influenza virus uses the hemagglutinin (HA and neuraminidase (NA glycoproteins to interact with and infect host cells. While biochemical and microscopic methods allow examination of the early steps in flu infection, the genesis of progeny virions has been more difficult to follow, mainly because of difficulties inherent in fluorescent labeling of flu proteins in a manner compatible with live cell imaging. We here apply sortagging as a chemoenzymatic approach to label genetically modified but infectious flu and track the flu glycoproteins during the course of infection. This method cleanly distinguishes influenza glycoproteins from host glycoproteins and so can be used to assess the behavior of HA or NA biochemically and to observe the flu glycoproteins directly by live cell imaging.

Intestinal mucus and juice glycoproteins have a liquid crystalline structure

International Nuclear Information System (INIS)

Denisova, E.A.; Lazarev, P.I.; Vazina, A.A.; Zheleznaya, L.A.

1985-01-01

X-ray diffraction patterns have been obtained from the following components of canine gastrointestinal tract: (1) native small intestine mucus layer; (2) the precipitate of the flocks formed in the duodenal juice with decreasing pH; (3) concentrated solutions of glycoproteins isolated from the duodenal juice. The X-ray patterns consist of a large number of sharp reflections of spacings between about 100 and 4 A. Some reflections are common for all components studied. All the patterns are interpreted as arising from the glycoprotein molecules ordered into a liquid crystalline structure. (author)

Use of λgt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella-zoster virus glycoproteins

International Nuclear Information System (INIS)

Petrovskis, E.A.; Timmins, J.G.; Post, L.E.

1986-01-01

A library of pseudorabies virus (PRV) DNA fragments was constructed in the expression cloning vector λgt11. The library was screened with antisera which reacted with mixtures of PRV proteins to isolate recombinant bacteriophages expressing PRV proteins. By the nature of the λgt11 vector, the cloned proteins were expressed in Escherichia coli as β-galactosidase fusion proteins. The fusion proteins from 35 of these phages were purified and injected into mice to raise antisera. The antisera were screened by several different assays, including immunoprecipitation of [ 14 C]glucosamine-labeled PRV proteins. This method identified phages expressing three different PRV glycoproteins: the secreted glycoprotein, gX; gI; and a glycoprotein that had not been previously identified, which we designate gp63. The gp63 and gI genes map adjacent to each other in the small unique region of the PRV genome. The DNA sequence was determined for the region of the genome encoding gp63 and gI. It was found that gp63 has a region of homology with a herpes simplex virus type 1 (HSV-1) protein, encoded by US7, and also with varicella-zoster virus (VZV) gpIV. The gI protein sequence has a region of homology with HSV-1 gE and VZV gpI. It is concluded that PRV, HSV, and VZV all have a cluster of homologous glycoprotein genes in the small unique components of their genomes and that the organization of these genes is conserved

Platelet Glycoprotein Ib-IX and Malignancy

Science.gov (United States)

2010-09-01

provide a unique microenvironment supporting the accumulation of more platelets and the elaboration of a fibrin - rich network produced by coagulation...process and can initiate the formation of a platelet - rich thrombus by tethering the platelet to a thrombogenic surface. Several ligands binding to GP Ib... Platelet Glycoprotein Ib-IX and Malignancy PRINCIPAL INVESTIGATOR: Jerry Ware, Ph.D

The quality control of glycoprotein folding in the endoplasmic reticulum, a trip from trypanosomes to mammals

Directory of Open Access Journals (Sweden)

A.J. Parodi

1998-05-01

Full Text Available The present review deals with the stages of synthesis and processing of asparagine-linked oligosaccharides occurring in the lumen of the endoplasmic reticulum and their relationship to the acquisition by glycoproteins of their proper tertiary structures. Special emphasis is placed on reactions taking place in trypanosomatid protozoa since their study has allowed the detection of the transient glucosylation of glycoproteins catalyzed by UDP-Glc:glycoprotein glucosyltransferase and glucosidase II. The former enzyme has the unique property of covalently tagging improperly folded conformations by catalyzing the formation of protein-linked Glc1Man7GlcNAc2, Glc1Man8GlcNac2 and Glc1Man9GlcNAc2 from the unglucosylated proteins. Glucosyltransferase is a soluble protein of the endoplasmic reticulum that recognizes protein domains exposed in denatured but not in native conformations (probably hydrophobic amino acids and the innermost N-acetylglucosamine unit that is hidden from macromolecular probes in most native glycoproteins. In vivo, the glucose units are removed by glucosidase II. The influence of oligosaccharides in glycoprotein folding is reviewed as well as the participation of endoplasmic reticulum chaperones (calnexin and calreticulin that recognize monoglucosylated species in the same process. A model for the quality control of glycoprotein folding in the endoplasmic reticulum, i.e., the mechanism by which cells recognize the tertiary structure of glycoproteins and only allow transit to the Golgi apparatus of properly folded species, is discussed. The main elements of this control are calnexin and calreticulin as retaining components, the UDP-Glc:glycoprotein glucosyltransferase as a sensor of tertiary structures and glucosidase II as the releasing agent.

Boronic Acid-Based Approach for Separation and Immobilization of Glycoproteins and Its Application in Sensing

Directory of Open Access Journals (Sweden)

Lin Liu

2013-10-01

Full Text Available Glycoproteins influence a broad spectrum of biological processes including cell-cell interaction, host-pathogen interaction, or protection of proteins against proteolytic degradation. The analysis of their glyco-structures and concentration levels are increasingly important in diagnosis and proteomics. Boronic acids can covalently react with cis-diols in the oligosaccharide chains of glycoproteins to form five- or six-membered cyclic esters. Based on this interaction, boronic acid-based ligands and materials have attracted much attention in both chemistry and biology as the recognition motif for enrichment and chemo/biosensing of glycoproteins in recent years. In this work, we reviewed the progress in the separation, immobilization and detection of glycoproteins with boronic acid-functionalized materials and addressed its application in sensing.

Molecular evidence of inefficient transduction of proliferating human B lymphocytes by VSV-pseudotyped HIV-1-derived lentivectors

International Nuclear Information System (INIS)

Serafini, M.; Naldini, L.; Introna, M.

2004-01-01

Lentiviral vectors are attractive tools to transduce dividing and nondividing cells. Human tonsillar B lymphocytes have been purified and induced to proliferate by the addition of anti-CD40 + IL-4 or anti-CD40 + anti-μ signals and transduced at high MOI with a VSV pseudotyped lentivector carrying the eGFP gene under the control of the PGK promoter. Parallel cultures of PHA-stimulated T lymphocytes containing a comparable amount of cycling cells during the infection reached over 70% eGFP transduction. By contrast, only less than 3% B lymphocytes became eGFP positive after 7 days from transduction. Molecular analysis of the viral life cycle shows that cytoplasmic retrotranscribed cDNA and nuclear 2LTR circles are detectable at lower levels and for a shorter period of time in proliferating B cells with respect to proliferating T lymphocytes. Moreover, FACS-sorted eGFP-positive and negative B cell populations were both positive for the presence of retrotranscribed cDNA and 2LTR circles nuclear forms. By contrast, nested Alu-LTR PCR allowed us to detect an integrated provirus in FACS-sorted eGFP-positive cells only. Together with the demonstration that infection in saturation conditions led to an increase in the percentage of transduced cells (reaching 9%), these findings suggest that in proliferating B lymphocytes, lentiviral transduction is an inefficient process blocked at the early steps of the viral life cycle possibly involving partially saturable restriction factors

An examination of the role of colonial Phaeocystis antarctica in the microbial food web of the Ross Sea

Science.gov (United States)

The extensive buildup of phytoplankton biomass in the Ross Sea conflicts with the view that high rates of herbivory occur in all regions of the Southern Ocean. Nano- and microplanktonic consumers comprise a significant fraction of total plankton biomass; however, the importance o...

Banking Competition and Efficiency: Empirical Analysis on the Bosnia and Herzegovina Using Panzar-Rosse Model

Directory of Open Access Journals (Sweden)

Memić Deni

2015-03-01

Full Text Available Background: Competition in the banking industry has been an important topic in the scientific literature as researchers tried to assess the level of competition in the banking sector. Objectives: This paper has an aim to investigate the market structure and a long term equilibrium of the banking market in Bosnia and Herzegovina nationwide as well as on its constitutional entities as well as to evaluate the monopoly power of banks during the years 2008-2012. Methods/Approach: The paper is examining the market structure using the most frequently applied measures of concentration k-bank concentration ratio (CRk and Herfindahl-Hirschman Index (HHI as well as evaluating the monopoly power of banks by employing Panzar-Rosse “H-statistic”. Results: The empirical results using CRk and HHI show that Bosnia and Herzegovina banking market has a moderately concentrated market with a concentration decreasing trend. The Panzar-Rosse “H-statistic” suggests that banks in Bosnia and Herzegovina operate under monopoly or monopolistic competition depending on the market segment. Conclusions: Banks operating on the banking market in Bosnia and Herzegovina seem to be earning their total and interest revenues under monopoly or perfectly collusive oligopoly.

STS-61B Astronaut Ross Works on Assembly Concept for Construction of Erectable Space Structure

Science.gov (United States)

1985-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo astronaut Ross, located on the Manipulator Foot Restraint (MFR) over the cargo bay, erects ACCESS. The primary objective of this experiment was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

STS-61B Astronauts Ross and Spring Work on Experimental Assembly of Structures in Extravehicular

Science.gov (United States)

1985-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia, and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). This STS-61B onboard photo depicts astronauts Ross and Spring working on EASE. The primary objective of these experiments was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Summer Distribution of Co2 Partial Pressure In The Ross Sea, Antarctica, and Relations With Biological Activity

Science.gov (United States)

Sandrini, S.; Tositti, L.; Tubertini, O.; Ceradini, S.; Palucci, A.; Barbini, R.; Fantoni, R.; Colao, F.; Ferrari, G. M.

The oceans play a key role in the processes responsible for global climate changes, in fact the oceanic uptake of anthropogenic atmospheric carbon dioxide is estimated to be 17-39The Southern Ocean and Antarctic marginal seas are considered to absorb up to half of this fraction. The Ross Sea, during the summer pack-ice melting, expe- riences rapid seasonal outgrowths, giving rise to phytoplankton blooms, especially in polynya areas near the coast line. This has a direct influence on pCO2 concentration in surface water, and hence on CO2 fluxes between ocean and atmosphere. Both the Ross Sea and the Southern Ocean transect between New Zealand and Antarctica are sys- tematically investigated during Italian Antarctic oceanographic campaigns onboard of the R/V Italica. During the XVI expedition, which took place in January and Febru- ary 2001, simultaneous measurements of surface pCO2 and Chlorophyll-a by laser remote-sensing apparatus were collected. Chlorophyll-a and pCO2 showed a general anticorrelation along the cruise. The survey has revealed the presence of high produc- tive regions in the polynya and close to the ice edge. The linear regression analysis of the chl-a vs pCO2 values improved our knowledge of the time evolution of the phyto- planktonic growth, independently measured by means of the laser yield, thus allowing for discrimination between different initial and final blooms in the Antarctic Ross Sea. The results obtained are here presented and discussed. They confirm the importance of biological production in the net absorption of atmospheric CO2 in continental shelf zones.

Temperature, salinity and oxygen data collected from the Oden in the Amundsen and Ross Seas, 2010 - 2011 (NCEI Accession 0104260)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Data were collected to investigate the physical oceanography of the Amundsen and Ross Seas. Funded under the following projects: Physical Oceanography of the...

Mucus glycoprotein secretion by tracheal explants: effects of pollutants

International Nuclear Information System (INIS)

Last, J.A.; Kaizu, T.

1980-01-01

Tracheal slices incubated with radioactive precursors in tissue culture medium secrete labeled mucus glycoproteins into the culture medium. We have used an in vivtro approach, a combined method utilizing exposure to pneumotoxins in vivo coupled with quantitation of mucus secretion rates in vitro, to study the effects of inhaled pollutants on mucus biosynthesis by rat airways. In addition, we have purified the mucus glycoproteins secreted by rat tracheal explants in order to determine putative structural changes that might by the basis for the observed augmented secretion rates after exposure of rats to H2SO4 aerosols in combination with high ambient levels of ozone. After digestion with papain, mucus glycoproteins secreted by tracheal explants may be separated into five fractions by ion-exchange chromatography, with recovery in high yield, on columns of DEAE-cellulose. Each of these five fractions, one neutral and four acidic, migrates as a single unique spot upon cellulose acetate electrophoresis at pH values of 8.6 and 1.2. The neutral fraction, which is labeled with [3H] glucosamine, does not contain radioactivity when Na2 35SO4 is used as the precursor. Acidic fractions I to IV are all labeled with either 3H-glucosamine or Na2 35SO4 as precursor. Acidic fraction II contains sialic acid as the terminal sugar on its oligosaccharide side chains, based upon its chromatographic behavior on columns of wheat-germ agglutinin-Agarose. Treatment of this fraction with neuraminidase shifts its elution position in the gradient to a lower salt concentration, coincident with acidic fraction I. After removal of terminal sialic acid residues with either neuraminidase or low pH treatment, the resultant terminal sugar on the oligosaccharide side chains is fucose. These results are identical with those observed with mucus glycoproteins secreted by cultured human tracheal explants and purified by these same techniques

Glycan structures contain information for the spatial arrangement of glycoproteins in the plasma membrane.

Directory of Open Access Journals (Sweden)

M Kristen Hall

Full Text Available Glycoconjugates at the cell surface are crucial for cells to communicate with each other and the extracellular microenvironment. While it is generally accepted that glycans are vectorial biopolymers, their information content is unclear. This report provides evidence that distinct N-glycan structures influence the spatial arrangement of two integral membrane glycoproteins, Kv3.1 and E-cadherin, at the adherent membrane which in turn alter cellular properties. Distinct N-glycan structures were generated by heterologous expression of these glycoproteins in parental and glycosylation mutant Chinese hamster ovary cell lines. Unlike the N-linked glycans, the O-linked glycans of the mutant cell lines are similar to those of the parental cell line. Western and lectin blots of total membranes and GFP immunopurified samples, combined with glycosidase digestion reactions, were employed to verify the glycoproteins had predominantly complex, oligomannose, and bisecting type N-glycans from Pro(-5, Lec1, and Lec10B cell lines, respectively. Based on total internal reflection fluorescence and differential interference contrast microscopy techniques, and cellular assays of live parental and glycosylation mutant CHO cells, we propose that glycoproteins with complex, oligomannose or bisecting type N-glycans relay information for localization of glycoproteins to various regions of the plasma membrane in both a glycan-specific and protein-specific manner, and furthermore cell-cell interactions are required for deciphering much of this information. These distinct spatial arrangements also impact cell adhesion and migration. Our findings provide direct evidence that N-glycan structures of glycoproteins contribute significantly to the information content of cells.

Two Case Histories, Ishbel Ross and Emma Bugbee: Women Journalists Ride the Rail with the Suffragettes.

Science.gov (United States)

Merrick, Beverly G.

The rise to prominence of the women's suffrage movement in the World War I years brought women reporters into U.S. newsrooms for the first time. In 1911 Emma Bugbee became the first woman hired as a "hard" news reporter for the "New York Tribune" (later the "Herald Tribune"). Ishbel Ross, author of "Ladies of the…

Design and synthesis of an antigenic mimic of the Ebola glycoprotein

OpenAIRE

Rutledge, Ryan D.; Huffman, Brian J.; Cliffel, David E.; Wright, David W.

2008-01-01

An antigenic mimic of the Ebola glycoprotein was synthesized and tested for its ability to be recognized by an anti-Ebola glycoprotein antibody. Epitope-mapping procedures yielded a suitable epitope that, when presented on the surface of a nanoparticle, forms a structure that is recognized by an antibody specific for the native protein. This mimic-antibody interaction has been quantitated through ELISA and QCM-based methods and yielded an affinity (Kd = 12 × 10−6 M) within two orders of magni...

Involvement of viral envelope GP2 in Ebola virus entry into cells expressing the macrophage galactose-type C-type lectin

International Nuclear Information System (INIS)

Usami, Katsuaki; Matsuno, Keita; Igarashi, Manabu; Denda-Nagai, Kaori; Takada, Ayato; Irimura, Tatsuro

2011-01-01

Highlights: → Ebola virus infection is mediated by binding to and fusion with the target cells. → Structural feature of the viral glycoprotein determines the infectivity. → Surface C-type lectin, MGL, of macrophages and dendritic cells mediate the infection. → GP2, one of glycoprotein subunits, plays an essential role in MGL-mediated infection. → There is a critical amino acid residue involved in high infectivity. -- Abstract: Ebola virus (EBOV) infection is initiated by the interaction of the viral surface envelope glycoprotein (GP) with the binding sites on target cells. Differences in the mortality among different species of the Ebola viruses, i.e., Zaire ebolavirus (ZEBOV) and Reston ebolavirus (REBOV), correspond to the in vitro infectivity of the pseudo-typed virus constructed with the GPs in cells expressing macrophage galactose-type calcium-type lectin (MGL/CD301). Through mutagenesis of GP2, the transmembrane-anchored subunit of GP, we found that residues 502-527 of the GP2 sequence determined the different infectivity between VSV-ZEBOV GP and -REBOV GP in MGL/CD301-expressing cells and a histidine residue at position 516 of ZEBOV GP2 appeared essential in the differential infectivity. These findings may provide a clue to clarify a molecular basis of different pathogenicity among EBOV species.

Involvement of viral envelope GP2 in Ebola virus entry into cells expressing the macrophage galactose-type C-type lectin

Energy Technology Data Exchange (ETDEWEB)

Usami, Katsuaki [Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033 (Japan); Matsuno, Keita; Igarashi, Manabu [Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 001-0020 (Japan); Denda-Nagai, Kaori [Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033 (Japan); Takada, Ayato [Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 001-0020 (Japan); Irimura, Tatsuro, E-mail: irimura@mol.f.u-tokyo.ac.jp [Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033 (Japan)

2011-04-01

Highlights: {yields} Ebola virus infection is mediated by binding to and fusion with the target cells. {yields} Structural feature of the viral glycoprotein determines the infectivity. {yields} Surface C-type lectin, MGL, of macrophages and dendritic cells mediate the infection. {yields} GP2, one of glycoprotein subunits, plays an essential role in MGL-mediated infection. {yields} There is a critical amino acid residue involved in high infectivity. -- Abstract: Ebola virus (EBOV) infection is initiated by the interaction of the viral surface envelope glycoprotein (GP) with the binding sites on target cells. Differences in the mortality among different species of the Ebola viruses, i.e., Zaire ebolavirus (ZEBOV) and Reston ebolavirus (REBOV), correspond to the in vitro infectivity of the pseudo-typed virus constructed with the GPs in cells expressing macrophage galactose-type calcium-type lectin (MGL/CD301). Through mutagenesis of GP2, the transmembrane-anchored subunit of GP, we found that residues 502-527 of the GP2 sequence determined the different infectivity between VSV-ZEBOV GP and -REBOV GP in MGL/CD301-expressing cells and a histidine residue at position 516 of ZEBOV GP2 appeared essential in the differential infectivity. These findings may provide a clue to clarify a molecular basis of different pathogenicity among EBOV species.

Involvement of Leishmania donovani major surface glycoprotein ...

Indian Academy of Sciences (India)

The major surface glycoprotein gp63 of the kinetoplastid protozoal parasite Leishmania is implicated as a ligand mediating uptake of the parasite into, and survival within, the host macrophage. By expressing gp63 antisense RNA from an episomal vector in L. donovani promastigotes, gp63-deficient transfectants were ...

Bioinformatics Analysis of Envelope Glycoprotein E epitopes of ...

African Journals Online (AJOL)

User

2011-05-02

May 2, 2011 ... 1National Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan. 2Department of ..... E glycoprotein and its interaction with antibody with the method of molecular dynamics and molecular model ...

eEF-2 Phosphorylation Down-Regulates P-Glycoprotein Over-Expression in Rat Brain Microvessel Endothelial Cells.

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Xing Hua Tang

Full Text Available We investigated whether glutamate, NMDA receptors, and eukaryote elongation factor-2 kinase (eEF-2K/eEF-2 regulate P-glycoprotein expression, and the effects of the eEF-2K inhibitor NH125 on the expression of P-glycoprotein in rat brain microvessel endothelial cells (RBMECs.Cortex was obtained from newborn Wistar rat brains. After surface vessels and meninges were removed, the pellet containing microvessels was resuspended and incubated at 37°C in culture medium. Cell viability was assessed by the MTT assay. RBMECs were identified by immunohistochemistry with anti-vWF. P-glycoprotein, phospho-eEF-2, and eEF-2 expression were determined by western blot analysis. Mdr1a gene expression was analyzed by RT-PCR.Mdr1a mRNA, P-glycoprotein and phospho-eEF-2 expression increased in L-glutamate stimulated RBMECs. P-glycoprotein and phospho-eEF-2 expression were down-regulated after NH125 treatment in L-glutamate stimulated RBMECs.eEF-2K/eEF-2 should have played an important role in the regulation of P-glycoprotein expression in RBMECs. eEF-2K inhibitor NH125 could serve as an efficacious anti-multidrug resistant agent.

Structure of a Pestivirus Envelope Glycoprotein E2 Clarifies Its Role in Cell Entry

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Kamel El Omari

2013-01-01

Full Text Available Enveloped viruses have developed various adroit mechanisms to invade their host cells. This process requires one or more viral envelope glycoprotein to achieve cell attachment and membrane fusion. Members of the Flaviviridae such as flaviviruses possess only one envelope glycoprotein, E, whereas pestiviruses and hepacivirus encode two glycoproteins, E1 and E2. Although E2 is involved in cell attachment, it has been unclear which protein is responsible for membrane fusion. We report the crystal structures of the homodimeric glycoprotein E2 from the pestivirus bovine viral diarrhea virus 1 (BVDV1 at both neutral and low pH. Unexpectedly, BVDV1 E2 does not have a class II fusion protein fold, and at low pH the N-terminal domain is disordered, similarly to the intermediate postfusion state of E2 from sindbis virus, an alphavirus. Our results suggest that the pestivirus and possibly the hepacivirus fusion machinery are unlike any previously observed.

Structure of a Pestivirus Envelope Glycoprotein E2 Clarifies Its Role in Cell Entry

Science.gov (United States)

El Omari, Kamel; Iourin, Oleg; Harlos, Karl; Grimes, Jonathan M.; Stuart, David I.

2013-01-01

Summary Enveloped viruses have developed various adroit mechanisms to invade their host cells. This process requires one or more viral envelope glycoprotein to achieve cell attachment and membrane fusion. Members of the Flaviviridae such as flaviviruses possess only one envelope glycoprotein, E, whereas pestiviruses and hepacivirus encode two glycoproteins, E1 and E2. Although E2 is involved in cell attachment, it has been unclear which protein is responsible for membrane fusion. We report the crystal structures of the homodimeric glycoprotein E2 from the pestivirus bovine viral diarrhea virus 1 (BVDV1) at both neutral and low pH. Unexpectedly, BVDV1 E2 does not have a class II fusion protein fold, and at low pH the N-terminal domain is disordered, similarly to the intermediate postfusion state of E2 from sindbis virus, an alphavirus. Our results suggest that the pestivirus and possibly the hepacivirus fusion machinery are unlike any previously observed. PMID:23273918

Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFα expression.

Science.gov (United States)

Müller, Mario M; Lehmann, Roland; Klassert, Tilman E; Reifenstein, Stella; Conrad, Theresia; Moore, Christoph; Kuhn, Anna; Behnert, Andrea; Guthke, Reinhard; Driesch, Dominik; Slevogt, Hortense

2017-04-12

Exposure of human monocytes to lipopolysaccharide (LPS) induces a temporary insensitivity to subsequent LPS challenges, a cellular state called endotoxin tolerance. In this study, we investigated the LPS-induced global glycoprotein expression changes of tolerant human monocytes and THP-1 cells to identify markers and glycoprotein targets capable to modulate the immunosuppressive state. Using hydrazide chemistry and LC-MS/MS analysis, we analyzed glycoprotein expression changes during a 48 h LPS time course. The cellular snapshots at different time points identified 1491 glycoproteins expressed by monocytes and THP-1 cells. Label-free quantitative analysis revealed transient or long-lasting LPS-induced expression changes of secreted or membrane-anchored glycoproteins derived from intracellular membrane coated organelles or from the plasma membrane. Monocytes and THP-1 cells demonstrated marked differences in glycoproteins differentially expressed in the tolerant state. Among the shared differentially expressed glycoproteins G protein-coupled receptor 84 (GPR84) was identified as being capable of modulating pro-inflammatory TNFα mRNA expression in the tolerant cell state when activated with its ligand Decanoic acid.

Evaluación del efecto de microorganismos eficientes en agua de bebida suministrada a pollos Ross X Ross en la granja Tunguavita

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Suren Angélica García-Vera

2011-02-01

Full Text Available El uso  de Microorganismos  Eficientes  (ME  en avicultura permite  aumentar  la productividad  y rentabilidad, además de mejorar las condiciones sanitarias de  la producción, convirtiéndolos en una técnica de suplementación sencilla, que permite optimizar  los  recursos para  el mejoramiento de las explotaciones avícolas. Con el fin de evaluar el  efecto de  los ME  sobre  algunos  parámetros productivos, se realizó este trabajo con pollos de engorde de la raza Ross X Ross. Se seleccionaron de  forma  aleatoria  90  aves,  las  cuales  fueron divididas  en  tres  grupos,  con  tres  repeticiones, respectivamente.  Al  tratamiento  uno  se  les administró ME durante  toda  la producción, a razón de 1 mL por cada 2.000 mL de agua (semanas 1, 7, 8 y 1mL por cada 1.000 mL de agua (semanas 2-6. Al  tratamiento  dos  se  les  administró ME durante toda la producción, a razón de 5 mL por cada 2.000 mL de agua (semanas 1, 7, 8 y 5 mL por  cada  1.000 mL  de  agua  (semanas 2-6.  El tratamiento  tres  correspondió  al  tratamiento control. Se realizó pesaje y recolección de heces cada  semana,  durante  las  8  semanas  de  la producción y se comprobó que en los grupos en los  que  se  usó ME  se  produjo  un  aumento significativo de conversión alimenticia y, por  lo tanto, de ganancia de peso en comparación con el  tratamiento control; en cuanto la concentración amoniacal en heces, ésta disminuyó notablemente en los tratamientos 1 ý 2.

Influence of Ross Sea Bottom Water changes on the warming and freshening of the Antarctic Bottom Water in the Australian-Antarctic Basin

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K. Shimada

2012-07-01

Full Text Available Changes to the properties of Antarctic Bottom Water in the Australian-Antarctic Basin (AA-AABW between the 1990s and 2000s are documented using data from the WOCE Hydrographic Program (WHP and repeated hydrographic surveys. Strong cooling and freshening are observed on isopycnal layers denser than γⁿ = 28.30 kg m⁻³. Changes in the average salinity and potential temperature below this isopycnal correspond to a basin-wide warming of 1300 ± 200 GW and freshening of 24 ± 3 Gt year⁻¹. Recent changes to dense shelf water in the source regions in the Ross Sea and George V Land can explain the freshening of AA-AABW but not its extensive warming. An alternative mechanism for this warming is a decrease in the supply of AABW from the Ross Sea (RSBW. Hydrographic profiles between the western Ross Sea and George V Land (171–158° E were analyzed with a simple advective-diffusive model to assess the causes of the observed changes. The model suggests that the warming of RSBW observed between the 1970s and 2000s can be explained by a 21 ± 23% reduction in RSBW transport and the enhancement of the vertical diffusion of heat resulting from a 30 ± 7% weakening of the abyssal stratification. The documented freshening of Ross Sea dense shelf water leads to a reduction in both salinity and density stratification. Therefore the direct freshening of RSBW at its source also produces an indirect warming of the RSBW. A simple box model suggests that the changes in RSBW properties and volume transport (a decrease of 6.7% is assumed between the year 1995 and 2005 can explain 51 ± 6% of the warming and 84 ± 10% of the freshening observed in AA-AABW.

STS-110 M.S. Ross in M-113 personnel carrier during TCDT

Science.gov (United States)

2002-01-01

KENNEDY SPACE CENTER, FLA. -- STS-110 Mission Specialist Jerry Ross waits his turn at driving the M-113 armored personnel carrier, part of Terminal Countdown Demonstration Test activities. In the background, right, is Mission Specialist Lee Morin. TCDT includes emergency egress training and a simulated launch countdown, and is held at KSC prior to each Space Shuttle flight. Scheduled for launch April 4, the 11-day mission will feature Shuttle Atlantis docking with the International Space Station (ISS) and delivering the S0 truss, the centerpiece-segment of the primary truss structure that will eventually extend over 300 feet.

Rhodocytin (aggretin) activates platelets lacking alpha(2)beta(1) integrin, glycoprotein VI, and the ligand-binding domain of glycoprotein Ibalpha

DEFF Research Database (Denmark)

Bergmeier, W; Bouvard, D; Eble, J A

2001-01-01

Although alpha(2)beta(1) integrin (glycoprotein Ia/IIa) has been established as a platelet collagen receptor, its role in collagen-induced platelet activation has been controversial. Recently, it has been demonstrated that rhodocytin (also termed aggretin), a snake venom toxin purified from the v...

Comparison of glycoprotein expression between ovarian and colon adenocarcinomas

DEFF Research Database (Denmark)

Multhaupt, H A; Arenas-Elliott, C P; Warhol, M J

1999-01-01

, carcinoembryonic antigen, and cytokeratins 7 and 20 to detect tumor-associated glycoproteins and keratin proteins in ovarian and colonic carcinomas. RESULTS: CA125, carcinoembryonic antigen, and cytokeratins 7 and 20 can distinguish between colonic and serous or endometrioid adenocarcinomas of the ovary in both...... primary and metastatic lesions. Mucinous ovarian adenocarcinomas differed in that they express carcinoembryonic antigen and cytokeratins 7 and 20 and weakly express CA125. The other glycoprotein antigens were equally expressed by ovarian and colonic adenocarcinomas and therefore were of no use...... in distinguishing between these 2 entities. CONCLUSION: A panel of monoclonal antibodies against cytokeratins 7 and 20 antigens, CA125, and carcinoembryonic antigen is useful in differentiating serous and endometrioid adenocarcinomas of the ovary from colonic adenocarcinomas. Mucinous ovarian adenocarcinomas cannot...

Extracellular Glycoproteins in Embryogenic Culture of Pumpkin (Cucurbita pepo L.

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Hana Čipčić Paljetak

2011-01-01

Full Text Available The extracellular proteins in three distinctly induced embryogenic lines of pumpkin (Cucurbita pepo L. cultivated in four MS media modified regarding the nitrogen composition or auxin presence/absence have been analyzed. Extracellular glycoproteins containing α-D-mannose were specifically detected by the lectine concavalin A. During the cultivation of embryogenic tissue in the medium supplemented with reduced nitrogen, the embryos were mostly arrested at preglobular and globular developmental stages, which coincide with the absence of protein secretion. Secreted glycoproteins of 76, 68, 37 and 34 kDa were detected only if any of the three lines were cultivated in the medium that stimulates embryo development, irrespectively of the addition of 2,4-dichlorophenoxyacetic acid or tunicamycin. The glycoprotein of 64 kDa was detected in all lines cultivated in hormone-free MS medium with conventional nitrogen sources and it appears to be associated with embryo maturation. Tunicamycin treatment did not influence embryogenesis, although it specifically affected glycosylation of proteins in the investigated lines. Our results show that besides auxin, the source of nitrate is of great importance for proper protein glycosylation, excretion and developmental transition of pumpkin somatic embryos.

A seasonal study of dissolved cobalt in the Ross Sea, Antarctica: micronutrient behavior, absence of scavenging, and relationships with Zn, Cd, and P

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M. A. Saito

2010-12-01

Full Text Available We report the distribution of cobalt (Co in the Ross Sea polynya during austral summer 2005–2006 and the following austral spring 2006. The vertical distribution of total dissolved Co (dCo was similar to soluble reactive phosphate (PO₄³⁻, with dCo and PO₄³⁻ showing a significant correlation throughout the water column (r² = 0.87, 164 samples. A strong seasonal signal for dCo was observed, with most spring samples having concentrations ranging from ~45–85 pM, whereas summer dCo values were depleted below these levels by biological activity. Surface transect data from the summer cruise revealed concentrations at the low range of this seasonal variability (~30 pM dCo, with concentrations as low as 20 pM observed in some regions where PO₄³⁻ was depleted to ~0.1 μM. Both complexed Co, defined as the fraction of dCo bound by strong organic ligands, and labile Co, defined as the fraction of dCo not bound by these ligands, were typically observed in significant concentrations throughout the water column. This contrasts the depletion of labile Co observed in the euphotic zone of other ocean regions, suggesting a much higher bioavailability for Co in the Ross Sea. An ecological stoichiometry of 37.6 μmol Co:mol⁻¹ PO₄³⁻ calculated from dissolved concentrations was similar to values observed in the subarctic Pacific, but approximately tenfold lower than values in the Eastern Tropical Pacific and Equatorial Atlantic. The ecological stoichiometries for dissolved Co and Zn suggest a greater overall use of Zn relative to Co in the shallow waters of the Ross Sea, with a Co:PO₄³⁻/Zn:PO₄³⁻ ratio of 1:17. Comparison of these observed stoichiometries with values estimated in culture studies suggests that Zn is a key micronutrient that likely influences phytoplankton diversity in the Ross

IgA antibodies against β2 glycoprotein I in hemodialysis patients are an independent risk factor for mortality.

Science.gov (United States)

Serrano, Antonio; García, Florencio; Serrano, Manuel; Ramírez, Elisa; Alfaro, F Javier; Lora, David; de la Cámara, Agustín Gómez; Paz-Artal, Estela; Praga, Manuel; Morales, Jose M

2012-06-01

Cardiovascular complications are the most important cause of death in patients on dialysis with end-stage renal disease. Antibodies reacting with β-glycoprotein I seem to play a pathogenic role in antiphospholipid syndrome and stroke and are involved in the origin of atherosclerosis. Here we evaluated the presence of anticardiolipin and anti-β-glycoprotein I antibodies together with other vascular risk factors and their relationship with mortality and cardiovascular morbidity in a cohort of 124 hemodialysis patients prospectively followed for 2 years. Of these, 41 patients were significantly positive for IgA anti-β-glycoprotein I, and the remaining had normal values. At 24 months, overall and cardiovascular mortality and thrombotic events were all significantly higher in patients with high anti-β-glycoprotein I antibodies. Multivariate analysis using Cox regression modeling found that age, hypoalbuminemia, use of dialysis catheters, and IgA β-glycoprotein I antibodies were independent risk factors for death. Thus, IgA antibodies to β-glycoprotein I are detrimental to the clinical outcome of hemodialysis patients.

Glycoprotein Ibalpha signalling in platelet apoptosis and clearance

NARCIS (Netherlands)

van der Wal, E.

2010-01-01

Storage of platelets at low temperature reduces bacterial growth and might better preserve the haemostatic function of platelets than current procedures. Incubation at 0C is known to expose ?-N-acetyl-D-glucosamine-residues on glycoprotein (GP)Ibalpha inducing receptor-clustering and platelet

Breaking Ice 2: A rift system on the Ross Ice Shelf as an analog for tidal tectonics on icy moons

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Brunt, K. M.; Hurford, T., Jr.; Schmerr, N. C.; Sauber, J. M.; MacAyeal, D. R.

2016-12-01

Ice shelves are the floating regions of the polar ice sheets. Outside of the influence of the narrow region of their grounding zone, they are fully hydrostatic and strongly influenced by the ocean tides. Recent observational and modeling studies have assessed the effect of tides on ice shelves, including: the tidal influence on the ice-shelf surface height, which changes by as much as 6 to 7 m on the southern extreme of the Ronne-Filchner Ice Shelf; the tidal modulation of the ice-shelf horizontal flow velocities, which changes the mean ice-flow rate by as much as two fold on the Ross Ice Shelf; and the tidal contribution to fracture and rift propagation, which eventually leads to iceberg calving. Here, we present the analysis of 16 days of continuous GPS data from a rift system near the front of the Ross Ice Shelf. While the GPS sites were installed for a different scientific investigation, and not optimized to assess tidal rifting mechanics, they provide a first-order sense of the tidal evolution of the rift system. These analyses can be used as a terrestrial analog for tidal activity on icy satellites, such as Europa and Enceladus, moons of Jupiter and Saturn, respectively. Using remote sensing and modeling of the Ross Ice Shelf rift system, we can investigate the geological processes observed on icy satellites and advance modeling efforts of their tidal-tectonic evolution.

Intra-seasonal variation in foraging behavior among Adélie penguins (Pygocelis adeliae) breeding at Cape Hallett, Ross Sea, Antarctica

Science.gov (United States)

Lyver, P.O.B.; MacLeod, C.J.; Ballard, G.; Karl, B.J.; Barton, K.J.; Adams, J.; Ainley, D.G.; Wilson, P.R.

2011-01-01

We investigated intra-seasonal variation in foraging behavior of chick-rearing Adélie penguins, Pygoscelis adeliae, during two consecutive summers at Cape Hallett, northwestern Ross Sea. Although foraging behavior of this species has been extensively studied throughout the broad continental shelf region of the Ross Sea, this is the first study to report foraging behaviors and habitat affiliations among birds occupying continental slope waters. Continental slope habitat supports the greatest abundances of this species throughout its range, but we lack information about how intra-specific competition for prey might affect foraging and at-sea distribution and how these attributes compare with previous Ross Sea studies. Foraging trips increased in both distance and duration as breeding advanced from guard to crèche stage, but foraging dive depth, dive rates, and vertical dive distances travelled per hour decreased. Consistent with previous studies within slope habitats elsewhere in Antarctic waters, Antarctic krill (Euphausia superba) dominated chick meal composition, but fish increased four-fold from guard to crèche stages. Foraging-, focal-, and core areas all doubled during the crèche stage as individuals shifted distribution in a southeasterly direction away from the coast while simultaneously becoming more widely dispersed (i.e., less spatial overlap among individuals). Intra-specific competition for prey among Adélie penguins appears to influence foraging behavior of this species, even in food webs dominated by Antarctic krill.

Lithology and chronology of ice-sheet fluctuations (magnetic susceptibility of cores from the western Ross Sea)

Science.gov (United States)

Jennings, Anne E.

1993-01-01

The goals of the marine geology part of WAIS include reconstructing the chronology and areal extent of ice-sheet fluctuations and understanding the climatic and oceanographic influences on ice-sheet history. As an initial step toward attaining these goals, down-core volume magnetic susceptibility (MS) logs of piston cores from three N-S transects in the western Ross Sea are compared. The core transects are within separate petrographic provinces based on analyses of till composition. The provinces are thought to reflect the previous locations of ice streams on the shelf during the last glaciation. Magnetic susceptibility is a function of magnetic mineral composition, sediment texture, and sediment density. It is applied in the western Ross Sea for two purposes: (1) to determine whether MS data differentiates the three transects (i.e., flow lines), and thus can be used to make paleodrainage reconstructions of the late Wisconsinan ice sheet; and (2) to determine whether the MS data can aid in distinguishing basal till diamictons from diamictons of glacial-marine origin and thus, aid paleoenvironmental interpretations. A comparison of the combined data of cores in each transect is presented.

The Fontaine Pluton : an early Ross Orogeny calc-alkaline gabbro from southern Victoria Land, Antarctica

International Nuclear Information System (INIS)

Cottle, J.M.; Cooper, A.F.

2006-01-01

The Fontaine Pluton is a previously undescribed mafic intrusion outcropping at Fontaine Bluff on the south side of the Carlyon Glacier in southern Victoria Land, Antarctica. It is the southern-most member of a laterally extensive mafic suite emplaced at mid-crustal depths during the initial stages of the Ross Orogeny. The pluton comprises recrystallised hornblende-biotite gabbro, which in places shows well-defined centimetre to metre scale primary igneous layering. Recrystallised ultramafic enclaves composed of amphibole-chlorite-talc are inferred to be remnants of a chemically and mineralogically distinct cumulate fraction. The intrusion has a 87 Sr/ 86 Sr (i) ratio of 0.70679 and a 143 Nd/ 144 Nd (i) ratio of 0.51187 (εNd (i) = -1.2). This, coupled with other geochemical data, implies that the Fontaine Pluton was formed by c. 15% partial melting of a depleted mantle source that was subsequently contaminated by continental crust. Preliminary U-Pb geochronology on zircon suggests an emplacement age for the pluton of 546 ± 10 Ma. These new data indicate that Ross Orogeny magmatism in this area of southern Victoria Land was initiated in the late Neoproterozoic along a subducting plate margin. (author). 55 refs., 10 figs., 3 tabs

Requirements within the Ebola Viral Glycoprotein for Tetherin Antagonism

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Nathan H. Vande Burgt

2015-10-01

Full Text Available Tetherin is an interferon-induced, intrinsic cellular response factor that blocks release of numerous viruses, including Ebola virus, from infected cells. As with many viruses targeted by host factors, Ebola virus employs a tetherin antagonist, the viral glycoprotein (EboGP, to counteract restriction and promote virus release. Unlike other tetherin antagonists such as HIV-1 Vpu or KSHV K5, the features within EboGP needed to overcome tetherin are not well characterized. Here, we describe sequences within the EboGP ectodomain and membrane spanning domain (msd as necessary to relieve tetherin restriction of viral particle budding. Fusing the EboGP msd to a normally secreted form of the glycoprotein effectively promotes Ebola virus particle release. Cellular protein or lipid anchors could not substitute for the EboGP msd. The requirement for the EboGP msd was not specific for filovirus budding, as similar results were seen with HIV particles. Furthermore trafficking of chimeric proteins to budding sites did not correlate with an ability to counter tetherin. Additionally, we find that a glycoprotein construct, which mimics the cathepsin-activated species by proteolytic removal of the EboGP glycan cap and mucin domains, is unable to counteract tetherin. Combining these results suggests an important role for the EboGP glycan cap and msd in tetherin antagonism.

Mapping Ross Ice Shelf with ROSETTA-Ice airborne laser altimetry

Science.gov (United States)

Becker, M. K.; Fricker, H. A.; Padman, L.; Bell, R. E.; Siegfried, M. R.; Dieck, C. C. M.

2017-12-01

The Ross Ocean and ice Shelf Environment and Tectonic setting Through Aerogeophysical surveys and modeling (ROSETTA-Ice) project combines airborne glaciological, geological, and oceanographic observations to enhance our understanding of the history and dynamics of the large ( 500,000 square km) Ross Ice Shelf (RIS). Here, we focus on the Light Detection And Ranging (LiDAR) data collected in 2015 and 2016. This data set represents a significant advance in resolution: Whereas the last attempt to systematically map RIS (the surface-based RIGGS program in the 1970s) was at 55 km grid spacing, the ROSETTA-Ice grid has 10-20 km line spacing and much higher along-track resolution. We discuss two different strategies for processing the raw LiDAR data: one that requires proprietary software (Riegl's RiPROCESS package), and one that employs open-source programs and libraries. With the processed elevation data, we are able to resolve fine-scale ice-shelf features such as the "rampart-moat" ice-front morphology, which has previously been observed on and modeled for icebergs. This feature is also visible in the ROSETTA-Ice shallow-ice radar data; comparing the laser data with radargrams provides insight into the processes leading to their formation. Near-surface firn state and total firn air content can also be investigated through combined analysis of laser altimetry and radar data. By performing similar analyses with data from the radar altimeter aboard CryoSat-2, we demonstrate the utility of the ROSETTA-Ice LiDAR data set in satellite validation efforts. The incorporation of the LiDAR data from the third and final field season (December 2017) will allow us to construct a DEM and an ice thickness map of RIS for the austral summers of 2015-2017. These products will be used to validate and extend observations of height changes from satellite radar and laser altimetry, as well as to update regional models of ocean circulation and ice dynamics.

Serum-SP/sub 1/-pregnancy-specific-. beta. -glycoprotein in choriocarcinoma and other neoplastic disease

Energy Technology Data Exchange (ETDEWEB)

Searle, F; Leake, B A; Bagshawe, K D; Dent, J [Charing Cross Group of Hospitals, London (UK)

1978-03-18

A radioimmunoassay for a placental glycoprotein, ..beta../sub 1/SP/sub 1/, capable of detecting 2 ..mu..g/l of the glycoprotein in serum was used to measure concentrations of ..beta../sub 1/SP/sub 1/ in patients with choriocarcinoma, teratoma, colonic cancer, breast cancer, and ovarian cancer. Twelve out of 94 (13%) healthy men and healthy non-pregnant women had detectable serum-..beta../sub 1/SP/sub 1/ concentrations. Concentrations up to 50,000 ..mu..g/l were found in the sera of patients with hydatidiform mole, invasive mole, choriocarcinoma, and malignant teratoma. ..beta../sub 1/-glycoprotein concentrations were generally much lower than corresponding concentrations of chorionic gonadotrophin which is the most reliable marker for trophoblastic tumors. In a few cases, however, ..beta../sub 1/-glycoprotein measurements may be useful in the detection of minimal residual tumor. The slightly raised values found in some patients with carcinoma of the colon, breast, or ovary seem unlikely to be useful for diagnostic purposes or for monitoring the course of these cancers.

Implications of Two Well-Known Models for Instructional Designers in Distance Education: Dick-Carey versus Morrison-Ross-Kemp

Science.gov (United States)

Akbulut, Yavuz

2007-01-01

This paper first summarizes, and then compares and contrasts two well-known instructional design models: Dick and Carey Model (DC) and Morrison, Ross and Kemp model (MRK). The target audiences of both models are basically instructional designers. Both models have applications for different instructional design settings. They both see the…

Structure of a trimeric variant of the Epstein-Barr virus glycoprotein B

Energy Technology Data Exchange (ETDEWEB)

Backovic, Marija [Northwestern Univ., Evanston, IL (United States); Longnecker, Richard [Northwestern Univ., Chicago, IL (United States); Jardetzky, Theodore S [Northwestern Univ., Evanston, IL (United States)

2009-03-16

Epstein-Barr virus (EBV) is a herpesvirus that is associated with development of malignancies of lymphoid tissue. EBV infections are life-long and occur in >90% of the population. Herpesviruses enter host cells in a process that involves fusion of viral and cellular membranes. The fusion apparatus is comprised of envelope glycoprotein B (gB) and a heterodimeric complex made of glycoproteins H and L. Glycoprotein B is the most conserved envelope glycoprotein in human herpesviruses, and the structure of gB from Herpes simplex virus 1 (HSV-1) is available. Here, we report the crystal structure of the secreted EBV gB ectodomain, which forms 16-nm long spike-like trimers, structurally homologous to the postfusion trimers of the fusion protein G of vesicular stomatitis virus (VSV). Comparative structural analyses of EBV gB and VSV G, which has been solved in its pre and postfusion states, shed light on gB residues that may be involved in conformational changes and membrane fusion. Also, the EBV gB structure reveals that, despite the high sequence conservation of gB in herpesviruses, the relative orientations of individual domains, the surface charge distributions, and the structural details of EBV gB differ from the HSV-1 protein, indicating regions and residues that may have important roles in virus-specific entry.

Ghost Stories: Review of Ross Gibson's The Summer Exercises

Directory of Open Access Journals (Sweden)

Deane Williams

2010-03-01

Full Text Available Back 
in 
1986, 
Ross 
Gibson
 was 
interviewed 
in
 Filmnews 
by
 Adrian
 Martin 
and
Tina
 Kaufman
 about
 his
 experimental
 short
 essay
 film
 Camera
 Natura
 (1984. Accompanying
 that
 interview
 was
 a
 photo
 of
 Gibson
 ‘on
 location’
 for
 his
 film,
 wearing
 a
 hat
 not
 unlike
 the
 one
 that
 features
 on
 the
 cover
 of
 his
 new
 book
 The
 Summer 
Exercises.
 Back 
then
 I
 understood
 the 
hat
 to 
be
 an
explorer’s,
 someone 
like
 Charles
 Sturt,
 someone
 whose
 willingness
 to
 learn
 from
 his
 environment
 had
 impressed
 Gibson
 in
 fashioning
 his
 own
 history
 of
 Australian
 landscape
 imaging.
 Now
 the
 hat
 looks
 like
 a
 detective’s
 or,
 rather,
 Marcel
 Duchamp’s
 rendering
 of
 a
 detective’s
 hat: 
on 
display,
 ready 
for
 our
 re‐consideration.

Pseudotyping Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV): F Proteins from Group II NPVs are functionally analogous to AcMNPV GP64

NARCIS (Netherlands)

Lung, O.; Westenberg, M.; Vlak, J.M.; Zuidema, D.; Blissard, G.W.

2002-01-01

GP64, the major envelope glycoprotein of budded virions of the baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), is involved in viral attachment, mediates membrane fusion during virus entry, and is required for efficient virion budding. Thus, GP64 is essential for viral

Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. AAA

Directory of Open Access Journals (Sweden)

Menzel Diedrik

2011-02-01

Full Text Available Abstract Background Hydroxyproline rich glycoproteins (HRGPs are implicated to have a role in many aspects of plant growth and development but there is limited knowledge about their localization and function during somatic embryogenesis of higher plants. In this study, the localization and function of hydroxyproline rich glycoproteins in embryogenic cells (ECs and somatic embryos of banana were investigated by using immunobloting and immunocytochemistry with monoclonal JIM11 and JIM20 antibodies as well as by treatment with 3,4-dehydro-L-proline (3,4-DHP, an inhibitor of extensin biosynthesis, and by immunomodulation with the JIM11 antibody. Results Immunofluorescence labelling of JIM11 and JIM20 hydroxyproline rich glycoprotein epitopes was relatively weak in non-embryogenic cells (NECs, mainly on the edge of small cell aggregates. On the other hand, hydroxyproline rich glycoprotein epitopes were found to be enriched in early embryogenic cells as well as in various developmental stages of somatic embryos. Embryogenic cells (ECs, proembryos and globular embryos showed strong labelling of hydroxyproline rich glycoprotein epitopes, especially in their cell walls and outer surface layer, so-called extracellular matrix (ECM. This hydroxyproline rich glycoprotein signal at embryo surfaces decreased and/or fully disappeared during later developmental stages (e.g. pear-shaped and cotyledonary stages of embryos. In these later developmental embryogenic stages, however, new prominent hydroxyproline rich glycoprotein labelling appeared in tri-cellular junctions among parenchymatic cells inside these embryos. Overall immunofluorescence labelling of late stage embryos with JIM20 antibody was weaker than that of JIM11. Western blot analysis supported the above immunolocalization data. The treatment with 3,4-DHP inhibited the development of embryogenic cells and decreased the rate of embryo germination. Embryo-like structures, which developed after 3,4-DHP

Rat macrophages: membrane glycoproteins in differentiation and function

NARCIS (Netherlands)

van den Berg, T. K.; Döpp, E. A.; Dijkstra, C. D.

2001-01-01

Macrophages (mphi) play a crucial role in the immune system. The rat offers unique advantages for studying the biology of mphi. Firstly, monoclonal antibodies (mAb) against many rat mphi surface glycoproteins have become available. These have not only demonstrated a considerable heterogeneity among

Interplay between HIV Entry and Transportin-SR2 Dependency

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Gijsbers Rik

2011-01-01

Full Text Available Abstract Background Transportin-SR2 (TRN-SR2, TNPO3, transportin 3 was previously identified as an interaction partner of human immunodeficiency virus type 1 (HIV-1 integrase and functions as a nuclear import factor of HIV-1. A possible role of capsid in transportin-SR2-mediated nuclear import was recently suggested by the findings that a chimeric HIV virus, carrying the murine leukemia virus (MLV capsid and matrix proteins, displayed a transportin-SR2 independent phenotype, and that the HIV-1 N74D capsid mutant proved insensitive to transportin-SR2 knockdown. Results Our present analysis of viral specificity reveals that TRN-SR2 is not used to the same extent by all lentiviruses. The DNA flap does not determine the TRN-SR2 requirement of HIV-1. We corroborate the TRN-SR2 independent phenotype of the chimeric HIV virus carrying the MLV capsid and matrix proteins. We reanalyzed the HIV-1 N74D capsid mutant in cells transiently or stably depleted of transportin-SR2 and confirm that the N74D capsid mutant is independent of TRN-SR2 when pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G. Remarkably, although somewhat less dependent on TRN-SR2 than wild type virus, the N74D capsid mutant carrying the wild type HIV-1 envelope required TRN-SR2 for efficient replication. By pseudotyping with envelopes that mediate pH-independent viral uptake including HIV-1, measles virus and amphotropic MLV envelopes, we demonstrate that HIV-1 N74D capsid mutant viruses retain partial dependency on TRN-SR2. However, this dependency on TRN-SR2 is lost when the HIV N74D capsid mutant is pseudotyped with envelopes mediating pH-dependent endocytosis, such as the VSV-G and Ebola virus envelopes. Conclusion Here we discover a link between the viral entry of HIV and its interaction with TRN-SR2. Our data confirm the importance of TRN-SR2 in HIV-1 replication and argue for careful interpretation of experiments performed with VSV-G pseudotyped viruses in

Electrophoretic demonstration of glycoproteins, lipoproteins, and phosphoproteins in human and bovine enamel

DEFF Research Database (Denmark)

Kirkeby, S; Moe, D; Bøg-Hansen, T C

1990-01-01

Enamel proteins from fully mineralized human molars and from bovine tooth germs were separated by electrophoresis. The gels were stained for detection of glycoproteins, lipoproteins, and phosphoproteins. Glycoproteins were shown by periodic acid-Schiff staining and lectin blotting. In mature human...... enamel a number of high molecular weight proteins could be demonstrated after ethylenediaminetetra-acetic acid demineralization and subsequent Triton X-100 extraction. These proteins are suggested to be lipoproteins. Phosphoproteins could only be visualized in enamel matrix from the tooth germs....

Argon Kα measurement on DIII endash D by Ross filters technique (abstract)

International Nuclear Information System (INIS)

Snider, R.T.; Bogatu, I.N.; Brooks, N.H.; Wade, M.R.

1999-01-01

Techniques to reduce the heat flux to the divertor plates in tokamak power plants and the consequent erosion of, and subsequent damage to the divertor target plates include the injection of impurities such as argon, that can dissipate the energy (through radiative or collisional processes) before it reaches the target plates. An important issue with this type of scheme is poisoning of the plasma core by the impurities introduced in the divertor region. Subsequently, there is a desire to measure the profiles of the injected impurities in the core. X-ray Ross filters with an effective narrow band pass centered on the argon Kα line at 3.2 keV, have been installed on two of the existing x-ray arrays on DIII endash D in order to help determine the argon concentration profiles. Emissivity profiles of the Kα lines and the emissivity profiles for the argon enhanced continuum can be inferred from the inverted filtered x-ray brightness signals if T e , n e , and Ar 18+ profiles are known. The MIST code is used to couple the filtered x-ray signals to the time dependent measurements of T e and n e . Further, the Ar 16+ profiles measured by charge transfer spectroscopy, are used as a constraint on the MIST code runs to calculate Ar 18+ profiles and unfold the argon emissivity profiles. A discussion of the Ross filters, the DIII endash D argon data, and the data analysis scheme for inferring argon emissivity profiles will be discussed. Estimates of the total argon concentration in the core determined from this technique in DIII endash D argon puff experiments will be presented. copyright 1999 American Institute of Physics

Effect of expression of P-glycoprotein on technetium-99m methoxyisobutylisonitrile single photon emission computed tomography of brain tumors

Energy Technology Data Exchange (ETDEWEB)

Shibata, Yasushi; Matsumura, Akira; Nose, Tadao [Tsukuba Univ., Ibaraki (Japan). Inst. of Clinical Medicine

2002-08-01

The expression of P-glycoprotein was investigated imunohistochemically in 26 brain tumor tissues and compared with the findings of technetium-99m methoxyisobutylisonitrile single photon emission computed tomography ({sup 99m}Tc-MIBI SPECT) to clarify the effect of P-glycoprotein on the diagnostic accuracy. P-glycoprotein labeling index of both tumor cells and vascular endothelial cells showed no clear relationship with the findings of {sup 99m}Tc-MIBI SPECT imaging. Expression of P-glycoprotein has no effect on the diagnostic accuracy of {sup 99m}Tc-MIBI SPECT. (author)

Changes in intestinal absorption of nutrients and brush border glycoproteins after total parenteral nutrition in rats.

Science.gov (United States)

Miura, S; Tanaka, S; Yoshioka, M; Serizawa, H; Tashiro, H; Shiozaki, H; Imaeda, H; Tsuchiya, M

1992-01-01

The effect of total parenteral nutrition on nutrients absorption and glycoprotein changes of brush border membrane was examined in rat small intestine. In total parenteral nutrition rats, a marked decrease in activity of brush border enzymes was observed mainly in the proximal and middle segments of the intestine. Galactose perfusion of jejunal segment showed that hexose absorption was significantly inhibited, while intestinal absorption of glycine or dipeptide, glycylglycine was not significantly affected by total parenteral nutrition treatment. When brush border membrane glycoprotein profile was examined by [3H]-glucosamine or [3H]-fucose incorporation into jejunal loops, significant changes were observed in the glycoprotein pattern of brush border membrane especially in the high molecular weight range over 120 kDa after total parenteral nutrition treatment, suggesting strong dependency of glycoprotein synthesis on luminal substances. Molecular weight of sucrase isomaltase in brush border membrane detected by specific antibody showed no significant difference, however, in total parenteral nutrition and control rats. Also, molecular weight of specific sodium glucose cotransporter of intestinal brush border membrane detected by selective photoaffinity labelling was not altered in total parenteral nutrition rats. It may be that prolonged absence of oral food intake may produce significant biochemical changes in brush border membrane glycoprotein and absorptive capacity of small intestine, but these changes were not observed in all brush border membrane glycoproteins. Images Figure 1 Figure 2 Figure 3 Figure 4 PMID:1582592

Carcinoma-specific Ulex europaeus agglutinin-I binding glycoproteins of human colorectal carcinoma and its relation to carcinoembryonic antigen.

Science.gov (United States)

Matsushita, Y; Yonezawa, S; Nakamura, T; Shimizu, S; Ozawa, M; Muramatsu, T; Sato, E

1985-08-01

Glycoproteins binding to Ulex europaeus agglutinin-I (UEA-I) lectin, which recognizes the terminal alpha-L-fucose residue, were analyzed in 18 cases of human colorectal carcinoma by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by the Western blotting method. In the distal large bowel (descending and sigmoid colon and rectum), high-molecular-weight glycoproteins binding to UEA-I existed in carcinoma tissue but not in normal mucosa. In the proximal large bowel (ascending and transverse colon), high-molecular-weight glycoproteins binding to UEA-I were found both in normal mucosa and in carcinoma tissue, whereas those from the carcinoma tissue had an apparently lower molecular weight as compared to the weight of those from the normal mucosa. Thus there is a biochemical difference in UEA-I binding glycoproteins between the normal mucosa and the carcinoma tissue, although in our previous histochemical study no difference was observed in UEA-I binding glycoproteins of the proximal large bowel between the carcinoma tissue and the normal mucosa. Furthermore, carcinoembryonic antigen from the carcinoma tissue was found to have the same electrophoretical mobility as the UEA-I binding glycoproteins.

Extra-oviductal expression of oviductal glycoprotein 1 in mouse ...

Indian Academy of Sciences (India)

2017-01-11

Jan 11, 2017 ... oestrogen-dependent protein, oviduct-specific glycoprotein. 1 (Ovgp1) also ... the tissues collected were testis, epididymis, prostate gland and seminal vesicle. ... The antigenic sites were unmasked by incuba- tion of sections ...

Coping with Dying: Lessons That We Should and Should Not Learn from the Work of Elisabeth Kubler-Ross.

Science.gov (United States)

Corr, Charles A.

1993-01-01

Appraises work of Elisabeth Kubler-Ross in area of coping with dying. Suggests lessons from that work. Draws broad conclusions about processes involved in coping with dying, argues on behalf of need to develop better theoretical models to explicate what is involved in coping with dying, and suggests requirements for model. (Author/NB)

Celastraceae sesquiterpenes as a new class of modulators that bind specifically to human P-glycoprotein and reverse cellular multidrug resistance.

Science.gov (United States)

Muñoz-Martínez, Francisco; Lu, Peihua; Cortés-Selva, Fernando; Pérez-Victoria, José María; Jiménez, Ignacio A; Ravelo, Angel G; Sharom, Frances J; Gamarro, Francisco; Castanys, Santiago

2004-10-01

Overexpression of ABCB1 (MDR1) P-glycoprotein, a multidrug efflux pump, is one mechanism by which tumor cells may develop multidrug resistance (MDR), preventing the successful chemotherapeutic treatment of cancer. Sesquiterpenes from Celastraceae family are natural compounds shown previously to reverse MDR in several human cancer cell lines and Leishmania strains. However, their molecular mechanism of reversion has not been characterized. In the present work, we have studied the ability of 28 dihydro-beta-agarofuran sesquiterpenes to reverse the P-glycoprotein-dependent MDR phenotype and elucidated their molecular mechanism of action. Cytotoxicity assays using human MDR1-transfected NIH-3T3 cells allowed us to select the most potent sesquiterpenes reversing the in vitro resistance to daunomycin and vinblastine. Flow cytometry experiments showed that the above active compounds specifically inhibited drug transport activity of P-glycoprotein in a saturable, concentration-dependent manner (K(i) down to 0.24 +/- 0.01 micromol/L) but not that of ABCC1 (multidrug resistance protein 1; MRP1), ABCC2 (MRP2), and ABCG2 (breast cancer resistance protein; BCRP) transporters. Moreover, sesquiterpenes inhibited at submicromolar concentrations the P-glycoprotein-mediated transport of [(3)H]colchicine and tetramethylrosamine in plasma membrane from CH(R)B30 cells and P-glycoprotein-enriched proteoliposomes, supporting that P-glycoprotein is their molecular target. Photoaffinity labeling in plasma membrane and fluorescence spectroscopy experiments with purified protein suggested that sesquiterpenes interact with transmembrane domains of P-glycoprotein. Finally, sesquiterpenes modulated P-glycoprotein ATPase-activity in a biphasic, concentration-dependent manner: they stimulated at very low concentrations but inhibited ATPase activity as noncompetitive inhibitors at higher concentrations. Sesquiterpenes from Celastraceae are promising P-glycoprotein modulators with potential

Glycoprotein H of herpes simplex virus type 1 requires glycoprotein L for transport to the surfaces of insect cells

NARCIS (Netherlands)

Westra, DF; Glazenburg, KL; Harmsen, MC; Tiran, A; Scheffer, AJ; Welling, GW; The, TH; WellingWester, S

In mammalian cells, formation of heterooligomers consisting of the glycoproteins H and L (gH and gL) of herpes simplex virus type 1 is essential for the cell-to-cell spread of virions and for the penetration of virions into cells. We examined whether formation of gH1/gL1 heterooligomers and cell

MDR1 P-glycoprotein transports endogenous opioid peptides

NARCIS (Netherlands)

Oude Elferink, R. P.; Zadina, J.

2001-01-01

MDR1 P-glycoprotein is generally regarded as an efflux pump for amphipathic toxic compounds. The question remains, however, whether certain endogenous compounds are also substrates for this transporter. Certain peptides have been shown to interact with MDR1 Pgp as well and we have therefore

Uma releitura da obra de Elisabeth Kubler-Ross

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Selene Beviláqua Chaves Afonso

2013-09-01

Full Text Available Este artigo apresenta uma releitura de parte da obra de Elizabeth Ross, uma das autoras mais citadas sobre a questão da terminalidade da vida, do luto e do morrer. Sua obra tem sido de grande contribuição tanto para os profissionais de saúde como para pais, mães, filhos, parentes, leigos e religiosos que vivenciam o luto. Também tem sido alvo de controvérsias relacionadas a questões éticas e quanto a seu rigor científico. Os livros aqui comentados são: On death and dying (Sobre A morte e o morrer, de 1969; Questions and answers on death and dying (Perguntas e respostas sobre a morte e o morrer, de 1971; Living with death and dying(Vivendo com a morte e os moribundos, de 1981; On children and death (Sobre as crianças e a morte, de 1983; On life after death (Sobre a vida depois da morte, de 1991 e Life lessons (Lições de vida, de 2000.

Assimilating bio-optical glider data during a phytoplankton bloom in the southern Ross Sea

Directory of Open Access Journals (Sweden)

D. E. Kaufman

2018-01-01

Full Text Available The Ross Sea is a region characterized by high primary productivity in comparison to other Antarctic coastal regions, and its productivity is marked by considerable variability both spatially (1–50 km and temporally (days to weeks. This variability presents a challenge for inferring phytoplankton dynamics from observations that are limited in time or space, which is often the case due to logistical limitations of sampling. To better understand the spatiotemporal variability in Ross Sea phytoplankton dynamics and to determine how restricted sampling may skew dynamical interpretations, high-resolution bio-optical glider measurements were assimilated into a one-dimensional biogeochemical model adapted for the Ross Sea. The assimilation of data from the entire glider track using the micro-genetic and local search algorithms in the Marine Model Optimization Testbed improves the model–data fit by  ∼ 50 %, generating rates of integrated primary production of 104 g C m−2 yr−1 and export at 200 m of 27 g C m−2 yr−1. Assimilating glider data from three different latitudinal bands and three different longitudinal bands results in minimal changes to the simulations, improves the model–data fit with respect to unassimilated data by  ∼ 35 %, and confirms that analyzing these glider observations as a time series via a one-dimensional model is reasonable on these scales. Whereas assimilating the full glider data set produces well-constrained simulations, assimilating subsampled glider data at a frequency consistent with cruise-based sampling results in a wide range of primary production and export estimates. These estimates depend strongly on the timing of the assimilated observations, due to the presence of high mesoscale variability in this region. Assimilating surface glider data subsampled at a frequency consistent with available satellite-derived data results in 40 % lower carbon export, primarily

A NEW ANALYSIS OF THE TWO CLASSICAL ZZ CETI WHITE DWARFS GD 165 AND ROSS 548. I. PHOTOMETRY AND SPECTROSCOPY

International Nuclear Information System (INIS)

Giammichele, N.; Fontaine, G.; Bergeron, P.; Brassard, P.; Charpinet, S.; Vauclair, G.; Pfeiffer, B.

2015-01-01

We present the first of a two-part seismic analysis of the two bright hot ZZ Ceti stars GD 165 and Ross 548. In this first part, we report the results of frequency extraction exercises based on time-series data sets of exceptional quality. We uncovered up to 13 independent pulsation modes in GD 165, regrouped into six main frequency multiplets. These include 9 secure (signal-to-noise ratio, S/N > 4) detections and 4 possible ones (4 ≥ S/N ≥ 3). Likewise, we isolated 11 independent modes in Ross 548 (9 secure and 2 possible detections), also regrouped into 6 multiplets. The multiplet structure is likely caused by rotational splitting. We also provide updated estimates of the time-averaged atmospheric properties of these two pulsators in the light of recent developments on the front of atmospheric modeling for DA white dwarfs

Expression of variable viruses as herpes simplex glycoprotein D and varicella zoster gE glycoprotein using a novel plasmid based expression system in insect cell

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A.M. Al-Sulaiman

2017-11-01

Full Text Available Several prokaryotic and eukaryotic expression systems have been used for in vitro production of viruses’ proteins. However eukaryotic expression system was always the first choice for production of proteins that undergo post-translational modification such as glycosylation. Recombinant baculoviruses have been widely used as safe vectors to express heterologous genes in the culture of insect cells, but the manipulation involved in creating, titrating, and amplifying viral stocks make it time consuming and laborious. Therefore, to facilitate rapid expression in insect cell, a plasmid based expression system was used to express herpes simplex type 1 glycoprotein D (HSV-1 gD and varicella zoster glycoprotein E (VZV gE. Recombinant plasmids were generated, transfected into insect cells (SF9, and both glycoproteins were expressed 48 h post-infection. A protein with approximately molecular weight of 64-kDa and 98-kDa for HSV-1 gD and VZV gE respectively was expressed and confirmed by SDS. Proteins were detected in insect cells cytoplasm and outer membrane by immunofluorescence. The antigenicity and immunoreactivity of each protein were confirmed by immunoblot and ELISA. Results suggest that this system can be an alternative to the traditional baculovirus expression for small scale expression system in insect cells.

The Ross classification for heart failure in children after 25 years: a review and an age-stratified revision.

Science.gov (United States)

Ross, Robert D

2012-12-01

Accurate grading of the presence and severity of heart failure (HF) signs and symptoms in infants and children remains challenging. It has been 25 years since the Ross classification was first used for this purpose. Since then, several modifications of the system have been used and others proposed. New evidence has shown that in addition to signs and symptoms, data from echocardiography, exercise testing, and biomarkers such as N-terminal pro-brain natriuretic peptide (NT-proBNP) all are useful in stratifying outcomes for children with HF. It also is apparent that grading of signs and symptoms in children is dependent on age because infants manifest HF differently than toddlers and older children. This review culminates in a proposed new age-based Ross classification for HF in children that incorporates the most useful data from the last two decades. Testing of this new system will be important to determine whether an age-stratified scoring system can unify the way communication of HF severity and research on HF in children is performed in the future.

Low rate doses effects of gamma radiation on glycoproteins of transmembrane junctions in fibroblasts

International Nuclear Information System (INIS)

Bringas, J.E.; Caceres, J.L.

1996-01-01

Glycoproteins of trans-membrane junctions are molecules that help to bind cells with the extracellular matrix. Integrins are the most important trans-membrane molecules among others. The damage of gamma radiation on those proteins could be an important early event that causes membrane abnormalities which may lead to cell malfunction and cancer induced by radiation due to cell dissociation. Randomized blocks with 3 repetitions of mouse embryo fibroblast cultures, were irradiated with Cobalt-60 gamma rays, during 20 days. Biological damage to glycoproteins and integrins was evaluated by cellular growth and fibroblast proliferative capacity. Integrins damage was studied by isolation by column immunoaffinity chromatography migrated on SDS-Page under reducing and non reducing conditions, and inhibition of integrins extracellular matrix adhesion by monoclonal antibodies effect. The dose/rate (0.05 Gy/day-0.2 Gy/day) of gamma given to cells did not show damage evidence on glycoproteins and integrins. If damage happened, it was repaired by cells very soon, was delayed by continuous cellular division or by glycoproteins characteristic of being multiple extracellular ligatures. Bio effects became more evident with an irradiation time greater than 20 days or a high dose/rate. (authors). 6 refs

RESEARCH OF COMPETITION IN DEPOSIT MARKET OF UKRAINE BASED ON THE PANZAR-ROSSE MODEL

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Iryna Didenko

2016-11-01

Full Text Available The problem in an adequate assessment of the level of competition in the banking market has prompted researchers to search constantly some new methods. The most famous of them were later successfully adapted to the banking market, are a models of Bresnahan, Panzar-Rosse, Bertrand, Iwat, Monti-Klein-Modesto Barros and others. In Ukraine, the process of assessing the level of competition in the banking sector is very relevant, in line with the recent reforms of particular relevance and distribution. Regarding the domestic deposit market, there is a shortage of qualitative analysis of this issue, unlike foreign practice. Therefore this study we try to solve this problem. The key goal of researchers in this study is in a detailed analysis to identify the important factors which make influence on the allocated markets, in particular on lending and deposit rates. Based on these factors there could be implemented internal adjustment processes taking place in the markets. The purpose of the article is to determine the level of competition in the deposit market of Ukraine in 2006-2015 basing on nonstructural Panzar- Rosse model and identify key factors that affect this level. Methodology. To research the level of competition in the deposit market of Ukraine the Panzar-Rosse model was used, in which separate 11 models were built using the integrated tool “Multiple regression” in the software package of statistics analysis STATISTICA. The input data in the model was presented with the information on financial performance of Ukrainian banks during ten years from 01.01.2005 till 01.01.2015. Results of this research showed that in Ukrainian deposit market there is a monopolistic competition, as proved by the calculated values of H-statistics index. The year of 2008, in the context of the global economic crisis, was marked as an unbalanced period for deposit market of Ukraine. Practical implications. Knowing the current state of competition level in the

Integrating terrestrial and marine records of the LGM in McMurdo Sound, Antarctica: implications for grounded ice expansion, ice flow, and deglaciation of the Ross Sea Embayment

Science.gov (United States)

Christ, A. J.; Marchant, D. R.

2017-12-01

During the LGM, grounded glacier ice filled the Ross Embayment and deposited glacial drift on volcanic islands and peninsulas in McMurdo Sound, as well as along coastal regions of the Transantarctic Mountains (TAM), including the McMurdo Dry Valleys and Royal Society Range. The flow geometry and retreat history of this ice remains debated, with contrasting views yielding divergent implications for both the fundamental cause of Antarctic ice expansion as well as the interaction and behavior of ice derived from East and West Antarctica during late Quaternary time. We present terrestrial geomorphologic evidence that enables the reconstruction of former ice elevations, ice-flow paths, and ice-marginal environments in McMurdo Sound. Radiocarbon dates of fossil algae interbedded with ice-marginal sediments provide a coherent timeline for local ice retreat. These data are integrated with marine-sediment records and multi-beam data to reconstruct late glacial dynamics of grounded ice in McMurdo Sound and the western Ross Sea. The combined dataset suggest a dominance of ice flow toward the TAM in McMurdo Sound during all phases of glaciation, with thick, grounded ice at or near its maximum extent between 19.6 and 12.3 calibrated thousands of years before present (cal. ka). Our data show no significant advance of locally derived ice from the TAM into McMurdo Sound, consistent with the assertion that Late Pleistocene expansion of grounded ice in McMurdo Sound, and throughout the wider Ross Embayment, occurs in response to lower eustatic sea level and the resulting advance of marine-based outlet glaciers and ice streams (and perhaps also reduced oceanic heat flux), rather than local increases in precipitation and ice accumulation. Finally, when combined with allied data across the wider Ross Embayment, which show that widespread deglaciation outside McMurdo Sound did not commence until 13.1 ka, the implication is that retreat of grounded glacier ice in the Ross Embayment did

A review on the relation between the brain-serum concentration ratio of drugs and the influence of P-glycoprotein

DEFF Research Database (Denmark)

Ejsing, Thomas Broeng; Morling, Niels; Linnet, Kristian

2007-01-01

This overview on the brain-serum relationship for drugs illustrates the importance of the drug transporter P-glycoprotein at the blood-brain barrier. Generally, an inverse relationship exists between the magnitude of the brain-serum ratio and the influence of P-glycoprotein. Concerning the pharma...... the pharmacogenomics of P-glycoprotein, no clear effect of single nucleotide polymorphisms (SNPs) has been demonstrated in humans....

Secretion of hepatitis C virus envelope glycoproteins depends on assembly of apolipoprotein B positive lipoproteins.

Directory of Open Access Journals (Sweden)

Vinca Icard

Full Text Available The density of circulating hepatitis C virus (HCV particles in the blood of chronically infected patients is very heterogeneous. The very low density of some particles has been attributed to an association of the virus with apolipoprotein B (apoB positive and triglyceride rich lipoproteins (TRL likely resulting in hybrid lipoproteins known as lipo-viro-particles (LVP containing the viral envelope glycoproteins E1 and E2, capsid and viral RNA. The specific infectivity of these particles has been shown to be higher than the infectivity of particles of higher density. The nature of the association of HCV particles with lipoproteins remains elusive and the role of apolipoproteins in the synthesis and assembly of the viral particles is unknown. The human intestinal Caco-2 cell line differentiates in vitro into polarized and apoB secreting cells during asymmetric culture on porous filters. By using this cell culture system, cells stably expressing E1 and E2 secreted the glycoproteins into the basal culture medium after one week of differentiation concomitantly with TRL secretion. Secreted glycoproteins were only detected in apoB containing density fractions. The E1-E2 and apoB containing particles were unique complexes bearing the envelope glycoproteins at their surface since apoB could be co-immunoprecipitated with E2-specific antibodies. Envelope protein secretion was reduced by inhibiting the lipidation of apoB with an inhibitor of the microsomal triglyceride transfer protein. HCV glycoproteins were similarly secreted in association with TRL from the human liver cell line HepG2 but not by Huh-7 and Huh-7.5 hepatoma cells that proved deficient for lipoprotein assembly. These data indicate that HCV envelope glycoproteins have the intrinsic capacity to utilize apoB synthesis and lipoprotein assembly machinery even in the absence of the other HCV proteins. A model for LVP assembly is proposed.

Panorama Pluton : a composite gabbro-monzodiorite early Ross Orogeny intrusion in southern Victoria Land, Antarctica

International Nuclear Information System (INIS)

Mellish, S.D.; Cooper, A.F.; Walker, N.W.

2002-01-01

The Koettlitz Glacier Alkaline Province of the Walcott Glacier to Radian Glacier area of the Transantarctic Mountains contains a diverse suite of intrusions ranging from gabbro and diorite to granite, nepheline syenite, and carbonatite. Most of the plutons are alkaline (A-type), although the Panorama Pluton is mafic, comprising both hypersthene normative gabbroic and quartz normative monzodioritic lithologies. The pluton has a composite nature, determined by whole-rock geochemical trends and Nd-Sr isotope data that reflect distinctive source regions for the different components. U-Pb geochronology of zircon and titanite indicates the Panorama Pluton was intruded during the early stages of the Neoproterozoic-early Paleozoic Ross Orogeny at 535 ± 9 Ma, and that it is coeval with the geochemically similar Dromedary Mafic Complex which crops out 10 km to the southeast. The Panorama Pluton is a volumetrically minor mafic component of the Koettlitz Glacier Alkaline Province, which predates, by at least 15 m.y., the dominant calc-alkaline suites that occur along-strike in the Dry Valleys area to the north, and the central Transantarctic Mountains to the south. The Panorama Pluton magmas, and other Koettlitz Glacier Alkaline Province lithologies, are interpreted to have formed in an extensional or transtensional jog that predates the onset of widespread Ross Orogeny subduction. (author). 48 refs., 7 figs., 3 tabs

A hybrid monolithic column based on boronate-functionalized graphene oxide nanosheets for online specific enrichment of glycoproteins.

Science.gov (United States)

Zhou, Chanyuan; Chen, Xiaoman; Du, Zhuo; Li, Gongke; Xiao, Xiaohua; Cai, Zongwei

2017-05-19

A hybrid monolithic column based on aminophenylboronic acid (APBA)-functionalized graphene oxide (GO) has been developed and used for selective enrichment of glycoproteins. The APBA/GO composites were homogeneously incorporated into a polymer monolithic column with the help of oligomer matrix and followed by in situ polymerization. The effect of dispersion of APBA/GO composites in the polymerization mixture on the performance of the monolithic column was explored in detail. The presence of graphene oxide not only enlarged the BET surface area from 6.3m 2 /g to 169.4m 2 /g, but also provided abundant boronic acid moieties for glycoprotein extraction, which improved the enrichment selectivity and efficiency for glycoproteins. The APBA/GO hybrid monolithic column was incorporated into a sequential injection system, which facilitated online extraction of proteins. Combining the superior properties of extraordinary surface area of GO and the affinity interaction of APBA to glycoproteins, the APBA/GO hybrid monolithic column showed higher enrichment factors for glycoproteins than other proteins without cis-diol-containing groups. Also, under comparable or even shorter processing time and without the addition of any organic solvent, it showed higher binding capacity toward glycoproteins compared with the conventional boronate affinity monolithic column. The practical applicability of this system was demonstrated by processing of egg white samples for extraction of ovalbumin and ovotransferrin, and satisfactory results were obtained by assay with SDS-PAGE. Copyright © 2017 Elsevier B.V. All rights reserved.

A Framework for the Evaluation and Reporting of Outcomes after Complex Heart Valve Interventions: Applications to the European Ross Registry

NARCIS (Netherlands)

E.I. Charitos (Efstratios)

2014-01-01

markdownabstract__Abstract__ Chapter 1 is the general introduction of the thesis. Chapter 2 presents a proposal on the nomenclature of the aortic root components. Chapters 3 and 4 present the single center results with the subcoronary Ross procedure in the University of Lübeck. Chapter 5

Thiolated polymers: evidence for the formation of disulphide bonds with mucus glycoproteins.

Science.gov (United States)

Leitner, Verena M; Walker, Greg F; Bernkop-Schnürch, Andreas

2003-09-01

Disulphide bonds between thiolated polymers (thiomers) and cysteine-rich subdomains of mucus glycoproteins are supposed to be responsible for the enhanced mucoadhesive properties of thiomers. This study set out to provide evidence for these covalent interactions using poly(acrylic acid)-cysteine conjugates of 2 and 450 kDa (PAA2-Cys, PAA450-Cys) displaying 402.5-776.0 micromol thiol groups per gram polymer. The effect of the disulphide bond breaker cysteine on thiomer-mucin disulphide bonds was monitored by (1) mucoadhesion studies and (2) rheological studies. Furthermore, (3) diffusion studies and (4) gel filtration studies were performed with thiomer-mucus mixtures. The addition of cysteine significantly (Ppolymer. Gel filtration studies showed that PAA2-Cys was able to form disulphide bonds with mucin glycoproteins resulting in an altered elution profile of the mucin/PAA2-Cys mixture in comparison to mucin alone or mucin/PAA2 mixture. According to these results, the study provides evidence for the formation of covalent bonds between thiomer and mucus glycoproteins.

Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection

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Mahmoud, Nora F. [Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan); Faculty of Pharmacy, Suez Canal University, Ismailia (Egypt); Jasirwan, Chyntia [Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan); Division of Hepatobiliary, Department of Internal Medicine, Faculty of Medicine, University of Indonesia (Indonesia); Kanemoto, Satoshi; Wakata, Aika; Wang, Bochao; Hata, Yuuki [Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan); Nagamata, Satoshi [Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan); Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe (Japan); Kawabata, Akiko [Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan); Tang, Huamin [Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan); Department of Immunology, Nanjing Medical University, Nanjing (China); Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.jp [Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan)

2016-03-15

Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in contrast to its revertant, could not be reconstituted. Moreover, deletion of gB cytoplasmic tail impaired the intracellular transport of gB protein to the trans-Golgi network (TGN). Taken together, these results suggest that gB CTD is critical for HHV-6 propagation and important for intracellular transportation. - Highlights: • Glycoprotein B (gB) is highly conserved among herpesviruses. • HHV-6 gB is also abundantly expressed in virions. • In the present study, we showed the function of HHV-6 gB cytoplasmic tail domain (CTD). • We found that deletion of gB CTD impairs the intracellular transport of gB protein to the trans-Golgi network (TGN), and CTD of gB is critical for HHV-6 propagation.

Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection

International Nuclear Information System (INIS)

Mahmoud, Nora F.; Jasirwan, Chyntia; Kanemoto, Satoshi; Wakata, Aika; Wang, Bochao; Hata, Yuuki; Nagamata, Satoshi; Kawabata, Akiko; Tang, Huamin; Mori, Yasuko

2016-01-01

Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in contrast to its revertant, could not be reconstituted. Moreover, deletion of gB cytoplasmic tail impaired the intracellular transport of gB protein to the trans-Golgi network (TGN). Taken together, these results suggest that gB CTD is critical for HHV-6 propagation and important for intracellular transportation. - Highlights: • Glycoprotein B (gB) is highly conserved among herpesviruses. • HHV-6 gB is also abundantly expressed in virions. • In the present study, we showed the function of HHV-6 gB cytoplasmic tail domain (CTD). • We found that deletion of gB CTD impairs the intracellular transport of gB protein to the trans-Golgi network (TGN), and CTD of gB is critical for HHV-6 propagation.

Neural glycoprotein M6a is released in extracellular vesicles and modulated by chronic stressors in blood

OpenAIRE

Monteleone, Melisa C.; Billi, Silvia C.; Brocco, Marcela A.; Frasch, Alberto C.

2017-01-01

Membrane neuronal glycoprotein M6a is highly expressed in the brain and contributes to neural plasticity promoting neurite growth and spine and synapse formation. We have previously showed that chronic stressors alter hippocampal M6a mRNA levels in rodents and tree shrews. We now show that M6a glycoprotein can be detected in mouse blood. M6a is a transmembrane glycoprotein and, as such, unlikely to be free in blood. Here we demonstrate that, in blood, M6a is transported in extracellular vesic...

Glycoprotein fucosylation is increased in seminal plasma of subfertile men

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Beata Olejnik

2015-04-01

Full Text Available Fucose, the monosaccharide frequent in N- and O-glycans, is a part of Lewis-type antigens that are known to mediate direct sperm binding to the zona pellucida. Such interaction was found to be inhibited in vitroby fucose-containing oligo- and polysaccharides, as well as neoglycoproteins. The objective of this study was to screen seminal plasma proteins of infertile/subfertile men for the content and density of fucosylated glycoepitopes, and compare them to samples of fertile normozoospermic subjects. Seminal proteins were separated in polyacrylamide gel electrophoresis and blotted onto nitrocellulose membrane and probed with fucose-specific Aleuria aurantia lectin (AAL. Twelve electrophoretic bands were selected for quantitative densitometric analysis. It was found that the content, and especially the density of fucosylated glycans, were higher in glycoproteins present in seminal plasma of subfertile men. No profound differences in fucosylation density were found among the groups of normozoospermic, oligozoospermic, asthenozoospermic, and oligoasthenozoospermic subfertile men. According to the antibody probing, AAL-reactive bands can be attributed to male reproductive tract glycoproteins, including prostate-specific antigen, prostatic acid phosphatase, glycodelin and chorionic gonadotropin. Fibronectin, α1 -acid glycoprotein, α1 -antitrypsin, immunoglobulin G and antithrombin III may also contribute to this high fucosylation. It is suggested that the abundant fucosylated glycans in the sperm environment could interfere with the sperm surface and disturb the normal course of the fertilization cascade.

Separation and identification of carp pituitary proteins and glycoproteins

Czech Academy of Sciences Publication Activity Database

Ryšlavá, H.; Janatová, M.; Čalounová, G.; Selicharová, Irena; Barthová, J.; Barth, Tomislav

2005-01-01

Roč. 50, č. 9 (2005), 430-437 ISSN 1212-1819 R&D Projects: GA MZe(CZ) QF3028 Institutional research plan: CEZ:AV0Z4055905 Keywords : carp hormones * glycoproteins * oligosaccharide chains Subject RIV: CE - Biochemistry Impact factor: 0.254, year: 2005

Human platelet glycoprotein IX: An adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures

International Nuclear Information System (INIS)

Hickey, M.J.; Williams, S.A.; Roth, G.J.

1989-01-01

The glycoprotein (GP) Ib-IX complex on the surface of human platelets functions as the von Willebrand factor receptor and mediates von Willebrand factor-dependent platelet adhesion to blood vessels. GPIX is a relatively small (M r , 17,000) protein that may provide for membrane insertion and orientation of the larger component of the complex. GPIb (M r , 165,000). Using antibody screening, the authors cloned a cDNA encoding GPIX from a human erythroleukemia cell cDNA library constructed in phage λgt11. Lacking a 5' untranslated region and start codon, the cDNA sequence includes 604 nucleotides, beginning with 495 bases at the 5' end coding for 165 amino acids, followed by a stop codon and 106 noncoding bases at the 3' end. By Northern blot analysis, the GPIX cDNA hybridizes with a single 1.0-kilobase species of platelet poly(A) + RNA. Translation of the cDNA sequence gives a predicted protein sequence beginning with a truncated putative signal sequence of 5 amino acids followed by a sequence of 17 amino acids matching that determined directly by Edman degradation of intact GPIX. GPIX contains a leucine-rich glycoprotein (LRG) sequence of 24 amino acids similar to conserved LRG sequences in GPIb and other proteins from humans, Drosophila, and yeast. The role of the flank-LRG center-flank structure in the evolution and function of the LRG proteins remains to be defined

Encoding asymmetry of the N-glycosylation motif facilitates glycoprotein evolution.

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Ryan Williams

Full Text Available Protein N-glycosylation is found in all domains of life and has a conserved role in glycoprotein folding and stability. In animals, glycoproteins transit through the Golgi where the N-glycans are trimmed and rebuilt with sequences that bind lectins, an innovation that greatly increases structural diversity and redundancy of glycoprotein-lectin interaction at the cell surface. Here we ask whether the natural tension between increasing diversity (glycan-protein interactions and site multiplicity (backup and status quo might be revealed by a phylogenic examination of glycoproteins and NXS/T(X ≠ P N-glycosylation sites. Site loss is more likely by mutation at Asn encoded by two adenosine (A-rich codons, while site gain is more probable by generating Ser or Thr downstream of an existing Asn. Thus mutations produce sites at novel positions more frequently than the reversal of recently lost sites, and therefore more paths though sequence space are made available to natural selection. An intra-species comparison of secretory and cytosolic proteins revealed a departure from equilibrium in sequences one-mutation-away from NXS/T and in (A content, indicating strong selective pressures and exploration of N-glycosylation positions during vertebrate evolution. Furthermore, secretory proteins have evolved at rates proportional to N-glycosylation site number, indicating adaptive interactions between the N-glycans and underlying protein. Given the topology of the genetic code, mutation of (A is more often nonsynonomous, and Lys, another target of many PTMs, is also encoded by two (A-rich codons. An examination of acetyl-Lys sites in proteins indicated similar evolutionary dynamics, consistent with asymmetry of the target and recognition portions of modified sites. Our results suggest that encoding asymmetry is an ancient mechanism of evolvability that increases diversity and experimentation with PTM site positions. Strong selective pressures on PTMs may have

Cancer Biomarker Discovery: Lectin-Based Strategies Targeting Glycoproteins

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David Clark

2012-01-01

Full Text Available Biomarker discovery can identify molecular markers in various cancers that can be used for detection, screening, diagnosis, and monitoring of disease progression. Lectin-affinity is a technique that can be used for the enrichment of glycoproteins from a complex sample, facilitating the discovery of novel cancer biomarkers associated with a disease state.

The Lyssavirus glycoprotein: A key to cross-immunity.

Science.gov (United States)

Buthelezi, Sindisiwe G; Dirr, Heini W; Chakauya, Ereck; Chikwamba, Rachel; Martens, Lennart; Tsekoa, Tsepo L; Stoychev, Stoyan H; Vandermarliere, Elien

2016-11-01

Rabies is an acute viral encephalomyelitis in warm-blooded vertebrates, caused by viruses belonging to Rhabdovirus family and genus Lyssavirus. Although rabies is categorised as a neglected disease, the rabies virus (RABV) is the most studied amongst Lyssaviruses which show nearly identical infection patterns. In efforts to improving post-exposure prophylaxis, several anti-rabies monoclonal antibodies (mAbs) targeting the glycoprotein (G protein) sites I, II, III and G5 have been characterized. To explore cross-neutralization capacity of available mAbs and discover new possible B-cell epitopes, we have analyzed all available glycoprotein sequences from Lyssaviruses with a focus on sequence variation and conservation. This information was mapped on the structure of a representative G protein. We proposed several possible cross-neutralizing B-cell epitopes (GUVTTTF, WLRTV, REECLD and EHLVVEEL) in complement to the already well-characterized antigenic sites. The research could facilitate development of novel cross-reactive mAbs against RABV and even more broad, against possibly all Lyssavirus members. Copyright © 2016 Elsevier Inc. All rights reserved.

Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates

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Se Chang Park

2013-01-01

Full Text Available Cyprinid herpes virus 3 (CyHV-3 diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio. Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116 of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116. In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies.

Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates

Science.gov (United States)

Han, Jee Eun; Kim, Ji Hyung; Renault, Tristan; Choresca, Casiano; Shin, Sang Phil; Jun, Jin Woo; Park, Se Chang

2013-01-01

Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies. PMID:23435236

Men of Good Will: The Religious Education Association, J. Elliot Ross, and the National Conference of Jews and Christians

Science.gov (United States)

Nolan, Lucinda A.

2009-01-01

An impetus of the Religious Education Association (REA) toward becoming an actively intercultural and interreligious agency emerged in the third decade of its existence. This article explores this period through an examination of the involvement of the REA members, Father John Elliot Ross and others (1884-1946) in a series of seminars conducted by…

Glycoprotein Ibα clustering in platelet storage and function

NARCIS (Netherlands)

Gitz, E.

2013-01-01

Platelets are anucleated, discoid-shaped cells that play an essential role in the formation of a hemostatic plug to prevent blood loss from injured vessels. Initial platelet arrest at the damaged arterial vessel wall is mediated through the interaction between the platelet receptor glycoprotein (GP)

Glucocorticoid-regulated and constitutive trafficking of proteolytically processed cell surface-associated glycoproteins in wild type and variant rat hepatoma cells

International Nuclear Information System (INIS)

Amacher, S.L.; Goodman, L.J.; Bravo, D.A.; Wong, K.Y.; Goldfine, I.D.; Hawley, D.M.; Firestone, G.L.

1989-01-01

Glucocorticoids regulate the trafficking of mouse mammary tumor virus (MMTV) glycoproteins to the cell surface in the rat hepatoma cell line M1.54, but not in the immunoselected sorting variant CR4. To compare the localization of MMTV glycoproteins to another proteolytically processed glycoprotein, both wild type M1.54 cells and variant CR4 cells were transfected with a human insulin receptor (hIR) expression vector, pRSVhIR. The production of cell surface hIR was monitored in dexamethasone-treated and -untreated wild type M1.54 and variant CR4 cells by indirect immunofluorescence, direct plasma membrane immunoprecipitation, and by [125I] insulin binding. In both wild type and variant rat hepatoma cells, hIR were localized at the cell surface in the presence or in the absence of 1 microM dexamethasone. In contrast, the glucocorticoid-regulated trafficking of cell surface MMTV glycoproteins occurred only in wild type M1.54 cells. We conclude that the hIR, which undergoes posttranslational processing reactions similar to MMTV glycoproteins, does not require glucocorticoids to be transported to the plasma membrane and is representative of a subset of cell surface glycoproteins whose trafficking is constitutive in rat hepatoma cells. Thus, MMTV glycoproteins and hIR provide specific cell surface markers to characterize the glucocorticoid-regulated and constitutive sorting pathways

BROILER´S ROSS 308 MEAT CHEMICAL COMPOSITION AFTER ADDITION OF BEE POLLEN AS A SUPPLEMENT IN THEIR FEED MIXTURES

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Peter Haščík

2014-02-01

Full Text Available The goal of this study was to evaluate the meat chemical composition of broiler chicken Ross 308. In the experiment, totally 200 one-day-old chicks were divided into 4 groups (n=50 for 42 days. Bee pollen was added to feed mixtures in doses 0; 2,500; 3,500 and 4,500 mg.kg-1. The findings found the moisture content of breast and thigh muscles were higher in the experimental groups compared to the control except E3 in thigh muscle, but in protein content were higher in the control group. On the other hand the fat content and energy value in the control group were higher comparison to experimental groups except E1. There are no significant differences (P ≥ 0.05 among the experimental groups. From the current study they conclude the bee pollen has a positive effect on the broiler Ross 308 chemical composition because the increase of moisture content and decrease the fat content which may be acceptable for several special human diets.

Replacement of the cytoplasmic domain alters sorting of a viral glycoprotein in polarized cells.

OpenAIRE

Puddington, L; Woodgett, C; Rose, J K

1987-01-01

The envelope glycoprotein (G protein) of vesicular stomatitis virus (VSV) is transported to the basolateral plasma membrane of polarized epithelial cells, whereas the hemagglutinin glycoprotein (HA protein) of influenza virus is transported to the apical plasma membrane. To determine if the cytoplasmic domain of VSV G protein might be important in directing G protein to the basolateral membrane, we derived polarized Madin-Darby canine kidney cell lines expressing G protein or G protein with i...

Efficacy of soluble glycoprotein fraction from Allium sativum purified by size exclusion chromatography on murine Schistosomiasis mansoni.

Science.gov (United States)

Aly, Ibrahim; Taher, Eman E; El-Sayed, Hoda; Mohammed, Faten A; ELnain, Gehan; Hamad, Rabab S; Bayoumy, Elsayed M

2017-06-01

In this work, the efficiency of crude MeOH extracts and soluble glycoprotein fraction of Allium sativum purified by size-exclusion chromatography (SEC) on parasitological, histopathological and some biochemical parameters in Schistosoma mansoni infected mice were investigated. Animals were infected by tail immersion with 100 cercariae/each mouse and divided into five groups in addition to the normal control. The results revealed a significant decrease in mean worm burden in all treated mice especially in the group treated with soluble glycoprotein fraction of A. sativum as compared to infected non-treated control with the disappearance of female worms. Administration of the studied extracts revealed remarkable amelioration in the levels of all the measured parameters in S. mansoni infected mice. In addition, treatment of mice with crude A. sativum MeOH extract and soluble glycoprotein fraction of A. sativum decreased significantly the activities of studied enzymes as compared to the infected untreated group. The highest degrees of enhancement in pathological changes was observed in the treated one with soluble glycoprotein fraction of A. sativum compared to the infected group represented by small sized, late fibro-cellular granuloma, the decrease in cellular constituents and degenerative changes in eggs. In conclusion, A. sativum treatment had effective schistosomicidal activities, through reduction of worm burden and tissue eggs, especially when it was given in purified glycoprotein fraction. Moreover, the soluble glycoprotein fraction of A. sativum largely modulates both the size and the number of granulomas. Copyright © 2017 Elsevier Ltd. All rights reserved.

High-throughput screening using pseudotyped lentiviral particles: a strategy for the identification of HIV-1 inhibitors in a cell-based assay.

Science.gov (United States)

Garcia, Jean-Michel; Gao, Anhui; He, Pei-Lan; Choi, Joyce; Tang, Wei; Bruzzone, Roberto; Schwartz, Olivier; Naya, Hugo; Nan, Fa-Jun; Li, Jia; Altmeyer, Ralf; Zuo, Jian-Ping

2009-03-01

Two decades after its discovery the human immunodeficiency virus (HIV) is still spreading worldwide and killing millions. There are 25 drugs formally approved for HIV currently on the market, but side effects as well as the emergence of HIV strains showing single or multiple resistances to current drug-therapy are causes for concern. Furthermore, these drugs target only 4 steps of the viral cycle, hence the urgent need for new drugs and also new targets. In order to tackle this problem, we have devised a cell-based assay using lentiviral particles to look for post-entry inhibitors of HIV-1. We report here the assay development, validation as well as confirmation of the hits using both wild-type and drug-resistant HIV-1 viruses. The screening was performed on an original library, rich in natural compounds and pure molecules from Traditional Chinese Medicine pharmacopoeia, which had never been screened for anti-HIV activity. The identified hits belong to four chemical sub-families that appear to be all non-nucleoside reverse transcriptase inhibitors (NNRTIs). Secondary tests with live viruses showed that there was good agreement with pseudotyped particles, confirming the validity of this approach for high-throughput drug screens. This assay will be a useful tool that can be easily adapted to screen for inhibitors of viral entry.

Recombinant pestivirus E2 glycoproteins prevent viral attachment to permissive and non permissive cells with different efficiency.

Science.gov (United States)

Asfor, A S; Wakeley, P R; Drew, T W; Paton, D J

2014-08-30

Bovine viral diarrhoea virus (BVDV) is an economically important animal pathogen, which like other pestiviruses has similar molecular biological features to hepaciviruses, including human Hepatitis C virus. The pestivirus E2 glycoproteins are the major target for virus-neutralising antibodies, as well as playing a role in receptor binding and host range restriction. In this study, recombinant E2 glycoproteins (rE2) derived from three different pestivirus species were examined for their inhibitory effects on pestivirus infectivity in cell culture. Histidine-tagged rE2 glycoproteins of BVDV type 2 strain 178003, BVDV type 1 strain Oregon C24V and CSFV strain Alfort 187 were produced in Spodoptera frugiperda insect cells and purified under native conditions. The ability of rE2 glycoprotein to inhibit the infection of permissive cells by both homologous and heterologous virus was compared, revealing that the inhibitory effects of rE2 glycoproteins correlated with the predicted similarity of the E2 structures in the recombinant protein and the test virus. This result suggests that the sequence and structure of E2 are likely to be involved in the host specificity of pestiviruses at their point of uptake into cells. Copyright © 2014 Elsevier B.V. All rights reserved.

Membrane glycoproteins of differentiating skeletal muscle cells

International Nuclear Information System (INIS)

Miller, K.R.; Remy, C.N.; Smith, P.B.

1987-01-01

The composition of N-linked glycoprotein oligosaccharides was studied in myoblasts and myotubes of the C2 muscle cell line. Oligosaccharides were radioactively labelled for 15 hr with [ 3 H] mannose and plasma membranes isolated. Ten glycopeptides were detected by SDS-PAGE and fluorography. The extent of labelling was 4-6 fold greater in myoblasts vs myotubes. A glycopeptide of Mr > 100,000 was found exclusively in myoblast membranes. Lectin chromatography revealed that the proportion of tri-, tetranntenary, biantennary and high mannose chains was similar throughout differentiation. The high mannose chain fraction was devoid of hybrid chains. The major high mannose chain contained nine mannose residues. The higher level of glycopeptide labelling in myoblasts vs myotubes corresponded to a 5-fold greater rate of protein synthesis. Pulse-chase experiments were used to follow the synthesis of the Dol-oligosaccharides. Myoblasts and myotubes labelled equivalently the glucosylated tetradecasaccharide but myoblasts labelled the smaller intermediates 3-4 greater than myotubes. Myoblasts also exhibited a 2-3 fold higher Dol-P dependent glycosyl transferase activity for chain elongation and Dol-sugar synthesis. Together these results show that the degree of protein synthesis and level of Dol-P are contributing factors in the higher capacity of myoblasts to produce N-glycoproteins compared to myotubes

Ross Sea Mollusca from the Latitudinal Gradient Program: R/V Italica 2004 Rauschert dredge samples

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Claudio Ghiglione

2013-10-01

Full Text Available Information regarding the molluscs in this dataset is based on the Rauschert dredge samples collected during the Latitudinal Gradient Program (LGP on board the R/V “Italica” in the Ross Sea (Antarctica in the austral summer 2004. A total of 18 epibenthic dredge deployments/samplings have been performed at four different locations at depths ranging from 84 to 515m by using a Rauschert dredge with a mesh size of 500µm. In total 8,359 specimens have been collected belonging to a total of 161 species. Considering this dataset in terms of occurrences, it corresponds to 505 discrete distributional records (incidence data. Of these, in order of abundance, 5,965 specimens were Gastropoda (accounting for 113 species, 1,323 were Bivalvia (accounting for 36 species, 949 were Aplacophora (accounting for 7 species, 74 specimens were Scaphopoda (3 species, 38 were Monoplacophora (1 species and, finally, 10 specimens were Polyplacophora (1 species. This data set represents the first large-scale survey of benthic micro-molluscs for the area and provides important information about the distribution of several species, which have been seldom or never recorded before in the Ross Sea. All vouchers are permanently stored at the Italian National Antarctic Museum (MNA, Section of Genoa, enabling future comparison and crosschecking. This material is also currently under study, from a molecular point of view, by the barcoding project “BAMBi” (PNRA 2010/A1.10.

The effect of propolis extract in the diet of chickens Ross 308 on their performance

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PETER HACK

2014-12-01

Full Text Available The focus of the study was the performance of Ross 308 chickens after the application of propolis extract in their diet. The nutritional value of feed mixtures for chickens was equal, but the experimental groups were also added propolis extract in the dose of 200 mg.kg-1 (E1, 300 mg.kg-1 (E2 and 400 mg.kg-1 (E3, respectively. The results of the experiments showed that the propolis extract in the examined concentrations can be applied to the diet of chickens, since it managed to raise (P = 0.05 the body weight positively (E1 2354.60 g; E2 2371.40 g; E3 2382.90 g, compared to control group (2272.89 g. Total increase of body weights indicates positive tendency of propolis extract in the diet of chickens Ross 308, because higher values (P = 0.05 were reached in the experimental groups (2311.60 g E1 to 2339.60 g E3 compared to control (2230.80 g. Total feed consumption was higher in the experimental groups, on the contrary, FCR was lower (P = 0.05 in the experimental groups (1.64 to 1.67 compared to control (1.69. The best performance results were reached in the group with the greatest addition of propolis extract, i.e. in the dose 400 mg.kg-1 of feed mixtures (E3.

Effect of an aqueous extract of Scoparia dulcis on plasma and tissue glycoproteins in streptozotocin induced diabetic rats.

Science.gov (United States)

Latha, M; Pari, L

2005-02-01

The influence of Scoparia dulcis, a traditionally used plant for the treatment of diabetes mellitus, was examined in streptozotocin diabetic rats on dearrangement in glycoprotein levels. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of streptozotocin. An aqueous extract of Scoparia dulcis plant was administered orally for 6 weeks. The effect of the Scoparia dulcis extract on blood glucose, plasma insulin, plasma and tissue glycoproteins studied was in comparison to glibenclamide. The levels of blood glucose and plasma glycoproteins were increased significantly whereas the level of plasma insulin was significantly decreased in diabetic rats. There was a significant decrease in the level of sialic acid and elevated levels of hexose, hexosamine and fucose in the liver and kidney of streptozotocin diabetic rats. Oral administration of Scoparia dulcis plant extract (SPEt) to diabetic rats led to decreased levels of blood glucose and plasma glycoproteins. The levels of plasma insulin and tissue sialic acid were increased whereas the levels of tissue hexose, hexosamine and fucose were near normal. The present study indicates that Scoparia dulcis possesses a significant beneficial effect on glycoproteins in addition to its antidiabetic effect.

Comparative Profiling of Triple-Negative Breast Carcinomas Tissue Glycoproteome by Sequential Purification of Glycoproteins and Stable Isotope Labeling

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Xiang Chen

2016-01-01

Full Text Available Background: Women with triple negative breast cancers (TNBCs have a poor prognosis due to lack of suitable targeted therapies. Changes in the protein glycosylation are increasingly being recognized as an important modification associated with cancer etiology. Methods: In an attempt to identify TNBC biomarkers with greater diagnostic and prognostic capabilities, hydrazide- based chemistry method combined with LC-MS/MS were used to purify and identify N-linked glycopeptides or glycoproteins of tissues from TNBC patients. Results: A total of 550 unique N-linked glycoproteins were identified, among these proteins, 72 unique N-linked glycoproteins were significantly regulated in tumor tissues, of which 56 proteins were upregulated and 16 proteins were downregulated. To assess the validity of the results, three selected proteins including Vascular endothelial growth factor receptor 1, Insulin receptor, Tissue factor pathway inhibitor were selected for western blot analysis, and these proteins were found as potential biomarkers of TNBC. The top three pathways of differentially expressed glycoproteins participated in were caveolar-mediated endocytosis signaling, agrin interactions at neuromuscular junction and LXR/RXR activation. Conclusion: This work provides potential glycoprotein markers to function as a novel tissue-based biomarker for TNBC.

Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography

Czech Academy of Sciences Publication Activity Database

Flodrová, Dana; Bobálová, Janette; Laštovičková, Markéta

2016-01-01

Roč. 44, č. 2 (2016), s. 286-297 ISSN 0133-3720 R&D Projects: GA ČR(CZ) GPP503/12/P395 Institutional support: RVO:68081715 Keywords : barley * wheat * glycoprotein * mass spectrometry * lectin chromatography Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 0.496, year: 2016

TROPHOBLASTIC β1 – GLYCOPROTEIN SYNTHESIS IN SEROPOSITIVE PREGNANT WOMEN

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R. N. Bogdanovich

2005-01-01

Full Text Available Abstract. The level of trophoblastic β1 – glycoprotein (SP–1 was determined in the blood sera of 200 healthy pregnant women and 184 women with threatened abortions in term till 20 weeks of pregnancy. In group of women experiencing recurrent abortions in 38 % cases antibodies to chorionic gonadotropin, in 39,5 % cases antibodies to phospholipids, in 25,5 % – antibodies to tireoglobulin were revealed in significant amounts. In 20,65 % lupus anticoagulant was found. The majority of women in this group had changes in homeostasis. The presence of autoantibodies during pregnancy is the unfavourable factor in the development of placental insufficiency. This is proved by the decreased secretion of trophoblastic β1 – glycoprotein – a marker of the fetal part of placenta. (Med. Immunol., 2005, vol.7, № 1, pp. 85588

Glycoprotein component of plant cell walls

International Nuclear Information System (INIS)

Cooper, J.B.; Chen, J.A.; Varner, J.E.

1984-01-01

The primary wall surrounding most dicotyledonous plant cells contains a hydroxyproline-rich glycoprotein (HRGP) component named extensin. A small group of glycopeptides solubilized from isolated cell walls by proteolysis contained a repeated pentapeptide glycosylated by tri- and tetraarabinosides linked to hydroxyproline and, by galactose, linked to serine. Recently, two complementary approaches to this problem have provided results which greatly increase the understanding of wall extensin. In this paper the authors describe what is known about the structure of soluble extensin secreted into the walls of the carrot root cells

Common Core State Standards and Teacher Effectiveness. Q&A with Ross Wiener, Ph.D. REL Mid-Atlantic Teacher Effectiveness Webinar Series

Science.gov (United States)

Regional Educational Laboratory Mid-Atlantic, 2013

2013-01-01

In this REL Mid-Atlantic webinar, Dr. Ross Wiener, Vice President and Executive Director of the Education and Society Program, Aspen Institute, discussed strategies for integrating the Common Core State Standards (CCSS) into teacher effectiveness systems, including ways in which the CCSS can support professional growth and inform teacher…

Albert Ross Tilley: The legacy of a Canadian plastic surgeon.

Science.gov (United States)

Mowbrey, Kevin

2013-01-01

The present article chronicles the career of Dr Albert Ross Tilley, one of the most important Canadian plastic surgeons of the 20th century. Tilley is most well known for his innovations of burn management during World War II and his treatment of a group of burn patients known affectionately as the 'Guinea Pig Club'. In addition to the superb surgical skills he applied to the physical wounds of his patients, Tilley was also a pioneer of caring for the emotional and psychological afflictions suffered by many airmen of World War II. As one of the founding fathers of the Canadian Society of Plastic Surgeons, Tilley's work was instrumental in establishing the specialty and ensured its prosperity for years to come. Serving in the capacity of leader, educator and innovator, Tilley remains one of Canada's most decorated physicians, and his body of work encompasses contributions to the medical field that remain significant and beneficial to patient care to this day.

The Dehumanizing American Dream in David Mamet’s Glengarry Glen Ross

Directory of Open Access Journals (Sweden)

Mehdi Sepehrmanesh

2014-01-01

Full Text Available The American Dream is a recurrent theme in American literature. In this response, this paper is an attempt to expose the destructive effects of the dream on the human spirit. It is also shown, through the analysis of David Mamet’s glengarry Glen Ross, that despite the promise of the dream it contains many contradictions. Beneath the seeming simple surface of the play lies a deep current of meanings that reflect the calamities of modern American life, and in a broader sense, the modern world. This article indicates how capitalism inculcates ideologies in the mind of individuals in order to facilitate the exploiting process and unquestioning subordination. Ragged individualism, for instance, as  the most prominent of these ideologies, disrupts all communal bonds and even exceeds to the disintegration of friendship and family life.

One new genus and three new species of deep-sea nematodes (Nematoda: Microlaimidae) from the Southwest Pacific Ocean and Ross Sea.

Science.gov (United States)

Leduc, Daniel

2016-02-11

New deep-sea nematodes of the family Microlaimidae are described from the Southwest Pacific Ocean and Ross Sea. Microlaimus korari n. sp. is characterised by annulated cuticle with longitudinal bars, round amphideal aperture slightly smaller than the cryptospiral amphideal fovea, spacious and heavily cuticularised buccal cavity with large dorsal tooth and right subventral tooth situated anteriorly relative to left subventral tooth, slender spicules 4.4 cloacal body diameters long, and gubernaculum 1.2 cloacal body diameters long with laterally curved distal end and swollen proximal end. Bolbolaimus tongaensis n. sp. is characterised by annulated cuticle with longitudinal bars, oval amphideal aperture and cryptocircular amphideal fovea situated between cephalic setae and only partially surrounded by cuticle annulations, and short spicules cuticularised along dorsal edge and at proximal end and with swollen portion near proximal end. Maragnopsia n. gen. is characterised by a minute, non-cuticularised mouth cavity without teeth, an elongated posterior pharyngeal bulb more than twice as long as it is wide, a single outstretched testis, and a conico-cylindrical tail 13-16 anal body diameters long. A list of all 83 valid Microlaimus species is provided. The present study provides the first microlaimid species records from deep-sea habitats (> 200 m depth) in the Southwest Pacific and Ross Sea. The presence of M. korari n. sp. on both the continental slope of New Zealand and Ross Sea abyssal plain suggests that this species has a wide geographical and depth distribution. However, molecular analyses will be required to confirm the identity of these two geographically disparate populations.

Appearance and cellular distribution of lectin-like receptors for alpha 1-acid glycoprotein in the developing rat testis

DEFF Research Database (Denmark)

Andersen, U O; Bøg-Hansen, T C; Kirkeby, S

1996-01-01

A histochemical avidin-biotin technique with three different alpha 1-acid glycoprotein glycoforms showed pronounced alterations in the cellular localization of two alpha 1-acid glycoprotein lectin-like receptors during cell differentiation in the developing rat testis. The binding of alpha 1-acid...

The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion

Energy Technology Data Exchange (ETDEWEB)

Ben Abdeljelil, Nawel; Rochette, Pierre-Alexandre; Pearson, Angela, E-mail: angela.pearson@iaf.inrs.ca

2013-09-15

Mutations in UL24 of herpes simplex virus type 1 can lead to a syncytial phenotype. We hypothesized that UL24 affects the sub-cellular distribution of viral glycoproteins involved in fusion. In non-immortalized human foreskin fibroblasts (HFFs) we detected viral glycoproteins B (gB), gD, gH and gL present in extended blotches throughout the cytoplasm with limited nuclear membrane staining; however, in HFFs infected with a UL24-deficient virus (UL24X), staining for the viral glycoproteins appeared as long, thin streaks running across the cell. Interestingly, there was a decrease in co-localized staining of gB and gD with F-actin at late times in UL24X-infected HFFs. Treatment with chemical agents that perturbed the actin cytoskeleton hindered the formation of UL24X-induced syncytia in these cells. These data support a model whereby the UL24 syncytial phenotype results from a mislocalization of viral glycoproteins late in infection. - Highlights: • UL24 affects the sub-cellular distribution of viral glycoproteins required for fusion. • Sub-cellular distribution of viral glycoproteins varies in cell-type dependent manner. • Drugs targeting actin microfilaments affect formation of UL24-related syncytia in HFFs.

Multiple genes encode the major surface glycoprotein of Pneumocystis carinii

DEFF Research Database (Denmark)

Kovacs, J A; Powell, F; Edman, J C

1993-01-01

The major surface antigen of Pneumocystis carinii, a life-threatening opportunistic pathogen in human immunodeficiency virus-infected patients, is an abundant glycoprotein that functions in host-organism interactions. A monoclonal antibody to this antigen is protective in animals, and thus this a...

Ross Sea Polynyas: Response of Ice Concentration Retrievals to Large Areas of Thin Ice

Science.gov (United States)

Kwok, R.; Comiso, J. C.; Martin, S.; Drucker, R.

2007-01-01

For a 3-month period between May and July of 2005, we examine the response of the Advanced Microwave Scanning Radiometer (AMSR-E) Enhanced NASA Team 2 (NT2) and AMSR-E Bootstrap (ABA) ice concentration algorithms to large areas of thin ice of the Ross Sea polynyas. Coincident Envisat Synthetic Aperture Radar (SAR) coverage of the region during this period offers a detailed look at the development of the polynyas within several hundred kilometers of the ice front. The high-resolution imagery and derived ice motion fields show bands of polynya ice, covering up to approximately 105 km(sup 2) of the Ross Sea, that are associated with wind-forced advection. In this study, ice thickness from AMSR-E 36 GHz polarization information serves as the basis for examination of the response. The quality of the thickness of newly formed sea ice (<10 cm) from AMSR-E is first assessed with thickness estimates derived from ice surface temperatures from the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument. The effect of large areas of thin ice in lowering the ice concentration estimates from both NT2/ABA approaches is clearly demonstrated. Results show relatively robust relationships between retrieved ice concentrations and thin ice thickness estimates that differ between the two algorithms. These relationships define the approximate spatial coincidence of ice concentration and thickness isopleths. Using the 83% (ABA) and 91% (NT2) isopleths as polynya boundaries, we show that the computed coverage compares well with that using the estimated 10-cm thickness contour. The thin ice response characterized here suggests that in regions with polynyas, the retrieval results could be used to provide useful geophysical information, namely thickness and coverage.

Ross filter pairs for metal artefact reduction in x-ray tomography: a case study based on imaging and segmentation of metallic implants

Science.gov (United States)

Arhatari, Benedicta D.; Abbey, Brian

2018-01-01

Ross filter pairs have recently been demonstrated as a highly effective means of producing quasi-monoenergetic beams from polychromatic X-ray sources. They have found applications in both X-ray spectroscopy and for elemental separation in X-ray computed tomography (XCT). Here we explore whether they could be applied to the problem of metal artefact reduction (MAR) for applications in medical imaging. Metal artefacts are a common problem in X-ray imaging of metal implants embedded in bone and soft tissue. A number of data post-processing approaches to MAR have been proposed in the literature, however these can be time-consuming and sometimes have limited efficacy. Here we describe and demonstrate an alternative approach based on beam conditioning using Ross filter pairs. This approach obviates the need for any complex post-processing of the data and enables MAR and segmentation from the surrounding tissue by exploiting the absorption edge contrast of the implant.

Molecular organization in bacterial cell membranes. Specific labelling and topological distribution of glycoproteins and proteins in Streptomyces albus membranes

Energy Technology Data Exchange (ETDEWEB)

Larraga, V; Munoz, E [Consejo Superior de Investigaciones Cientificas, Madrid (Spain). Instituto de Biologia Celular

1975-05-01

The paper reports about an investigation into the question of the specific labelling and topological distribution of glycoproteins and proteins in Streptomyces albus membranes. The method of sample preparation is described: Tritium labelling of glycoproteins in protoplasts and membranes, iodination of proteins, trypsin treatment and polyacrylamide gel electrophoresis. The findings suggest an asymmetrical distribution of the glycoproteins in membranes and a weak accessibility to iodine label. A structural model of the plasma membranes of Streptomyces albus is proposed similar to the general 'fluid mosaic' model of Singer and Nicholson.

Generating Isoform-Specific Antibodies : Lessons from Nucleocytoplasmic Glycoprotein Skp1

NARCIS (Netherlands)

West, Christopher M.; Van Der Wel, Hanke; Chinoy, Zoiesha; Boons, Geert Jan; Gauthier, Ted J.; Taylor, Carol M.; Xu, Yuechi

2015-01-01

Antibodies that discriminate protein isoforms differing by modifications at specific amino acids have revolutionized studies of their functions. Skp1 is a novel nucleocytoplasmic glycoprotein that is hydroxylated at proline-143 and then O-glycosylated by a pentasaccharide attached via a GlcNAcα1,

Direct chemical modification and voltammetric detection of glycans in glycoproteins

Czech Academy of Sciences Publication Activity Database

Trefulka, Mojmír; Paleček, Emil

2014-01-01

Roč. 48, NOV2014 (2014), s. 52-55 ISSN 1388-2481 R&D Projects: GA ČR(CZ) GAP301/11/2055 Institutional support: RVO:68081707 Keywords : Glycoproteins * Chemical modification * Os(VI)L complexes Subject RIV: BO - Biophysics Impact factor: 4.847, year: 2014

Irradiation of rat brain reduces P-glycoprotein expression and function

NARCIS (Netherlands)

Bart, J.; Nagengast, W. B.; Coppes, R. P.; Wegman, T. D.; van der Graaf, W. T. A.; Groen, H. J. M.; Vaalburg, W.; de Vries, E. G. E.; Hendrikse, N. H.

2007-01-01

The blood - brain barrier ( BBB) hampers delivery of several drugs including chemotherapeutics to the brain. The drug efflux pump P- glycoprotein ( P- gp), expressed on brain capillary endothelial cells, is part of the BBB. P- gp expression on capillary endothelium decreases 5 days after brain

Moses presages Kubler-Ross: five stages in accepting death, as seen in the midrash.

Science.gov (United States)

Berkowitz, M C; Knight, L

2001-11-01

This paper elucidates and explains an ancient midrash (rabbinic interpretation of a biblical text) through the lens of modern psychological theory. The midrash describes Moses' reactions to his approaching death. The paper points out that these reactions anticipate the five classic stages, described by Elizabeth Kubler-Ross, of coming to accept terminal illness: denial, anger, bargaining, depression and acceptance. The article shows the ancient rabbis' sensitivity to human feeling and the universal nature of human reaction through the dialogue and reactions they attribute to Moses. Finally, it shows how using this midrash offers a constructive model for approaching death, for Jewish and non-Jewish patients alike, as well as their caregivers.

STS-110 M.S. Ross and Smith in M-113 personnel carrier during TCDT

Science.gov (United States)

2002-01-01

KENNEDY SPACE CENTER, FLA. -- With STS-110 Mission Specialists Jerry Ross (far left) and Steven Smith (third from left) on board, Commander Michael Bloomfield scatters dust as he practices driving the M-113 armored personnel carrier. The driving is part of Terminal Countdown Demonstration Test activities, which include emergency egress training and a simulated launch countdown. The TCDT is held at KSC prior to each Space Shuttle flight. Scheduled for launch April 4, the 11-day mission will feature Shuttle Atlantis docking with the International Space Station (ISS) and delivering the S0 truss, the centerpiece-segment of the primary truss structure that will eventually extend over 300 feet.

STS-55 MS1/PLC Ross monitors Payload Specialist Walter's Anthrorack activity

Science.gov (United States)

1993-01-01

STS-55 German Payload Specialist 1 Ulrich Walter breathes into Rack 9 Anthrorack (AR) (Human Physiology Laboratory) device for Pulmonary Perfusion and Ventilation During Rest and Exercise experiment while working inside the Spacelab Deutsche 2 (SL-D2) science module aboard the Earth-orbiting Columbia, Orbiter Vehicle (OV) 102. Seated on the bicycle ergometer, Walter utilizes the respiratory monitoring system, part of a broad battery of experiments designed to investigate human physiology under microgravity conditions. In the background, Mission Specialist 1 (MS1) and Payload Commander (PLC) Jerry L. Ross monitors Walter's activity. Walter represents the German Aerospace Research Establishment (DLR) on the 10-day SL-D2 mission. Visible on the aft end cone are a fire extinguisher and the Crew Telesupport Experiment (CTE) Macintosh portable computer mounted on an adjustable work platform.

Rapid Holocene thinning of outlet glaciers followed by readvance in the western Ross Embayment, Antarctica

Science.gov (United States)

Jones, R. S.; Whitmore, R.; Mackintosh, A.; Norton, K. P.; Eaves, S.; Stutz, J.

2017-12-01

Investigating Antarctic deglaciation following the LGM provides an opportunity to better understand patterns, mechanisms and drivers of ice sheet retreat. In the Ross Sea sector, geomorphic features preserved on the seafloor indicate that streaming East Antarctic outlet glaciers once extended >100 km offshore of South Victoria Land prior to back-stepping towards their modern configurations. In order to adequately interpret the style and causes of this retreat, the timing and magnitude of corresponding ice thickness change is required. We present new constraints on ice surface lowering from Mawson Glacier, an outlet of the East Antarctic Ice Sheet that flows into the western Ross Sea. Surface-exposure (10Be) ages from samples collected in elevation transects above the modern ice surface reveal that rapid thinning occurred at 5-8 ka, broadly coeval with new ages of grounding-line retreat at 6 ka and rapid thinning recorded at nearby Mackay Glacier at 7 ka. Our data also show that a moraine formed near to the modern ice margin of Mawson Glacier at 0.8 ka, which, together with historical observations, indicates that glaciers in this region readvanced during the last thousand years. We argue that 1) the accelerated thinning of outlet glaciers was driven by local grounding-line retreat through overdeepened basins during the early-mid Holocene, and 2) the glaciers subsequently readvanced, possibly linked to late Holocene sea-ice expansion, before retreating to their current positions. Our work demonstrates that these outlet glaciers were closely coupled to environmental and topography-induced perturbations near their termini throughout the Holocene.

An unusual dependence of human herpesvirus-8 glycoproteins-induced cell-to-cell fusion on heparan sulfate

International Nuclear Information System (INIS)

Tiwari, Vaibhav; Darmani, Nissar A.; Thrush, Gerald R.; Shukla, Deepak

2009-01-01

Human herpesvirus-8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins-induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from following lines of evidence: (1) a significant reduction in cell-to-cell fusion occurred when target cells were treated with heparinase; (2) in a competition assay, when the effector cells expressing HHV-8 glycoproteins were challenged with soluble HS, cell-to-cell fusion was reduced; and, (3) co-expression of HHV-8 glycoproteins gH-gL on target cells resulted in inhibition of cell surface HS expression. Taken together, our results indicate that cell surface HS can play an additional role during HHV-8 pathogenesis.

An unusual dependence of human herpesvirus-8 glycoproteins-induced cell-to-cell fusion on heparan sulfate

Energy Technology Data Exchange (ETDEWEB)

Tiwari, Vaibhav [Department of Ophthalmology, University of Illinois at Chicago, Chicago, IL 60612 (United States); Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612 (United States); Department of Basic Medical Sciences, College of Osteopathic Medicine of the Pacific and College of Optometry, Western University of Health Sciences, Pomona, CA 91766 (United States); Darmani, Nissar A.; Thrush, Gerald R. [Department of Basic Medical Sciences, College of Osteopathic Medicine of the Pacific and College of Optometry, Western University of Health Sciences, Pomona, CA 91766 (United States); Shukla, Deepak, E-mail: dshukla@uic.edu [Department of Ophthalmology, University of Illinois at Chicago, Chicago, IL 60612 (United States); Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612 (United States)

2009-12-18

Human herpesvirus-8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins-induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from following lines of evidence: (1) a significant reduction in cell-to-cell fusion occurred when target cells were treated with heparinase; (2) in a competition assay, when the effector cells expressing HHV-8 glycoproteins were challenged with soluble HS, cell-to-cell fusion was reduced; and, (3) co-expression of HHV-8 glycoproteins gH-gL on target cells resulted in inhibition of cell surface HS expression. Taken together, our results indicate that cell surface HS can play an additional role during HHV-8 pathogenesis.

Effect of penguin and seal excrement on mercury distribution in sediments from the Ross Sea region, East Antarctica.

Science.gov (United States)

Nie, Yaguang; Liu, Xiaodong; Sun, Liguang; Emslie, Steven D

2012-09-01

Total mercury (Hg) concentration and several other geochemical parameters were determined for five sediment profiles from the Antarctic Ross Sea region. Our data exhibit significant positive correlations between Hg concentration and total organic carbon (TOC) content in all profiles, suggesting the predominant role of organic matter (OM) as a Hg carrier. The OM in the sediments originates primarily from penguin guano and algae. High Hg content in guano and a positive correlation between Hg and a guano bio-element (phosphorus, P) in the ornithogenic sediment profiles (MB6, BI and CC) indicate that Hg was strongly influenced by guano input. The bottom sediments of MB6 with seal hairs contain relatively high Hg. This increase is attributed to the input of seal excrement, suggesting that sedimentary Hg may be an effective trophic-level indicator from seals to penguins. The enrichment factor (EF) for Hg was calculated and the results indicated apparent Hg enrichment in the sediment profiles from the Ross Sea region caused by bio-vectors such as penguins and seals. Compared with typical sediments from other sites in Antarctica and the SQGs (sediment quality guidelines), the total amount of Hg in our study area is still not considered to be adversely high. Copyright © 2012 Elsevier B.V. All rights reserved.

Contribution of the attachment G glycoprotein to pathogenicity and immunogenicity of avian metapneumovirus subgroup C.

Science.gov (United States)

Govindarajan, Dhanasekaran; Kim, Shin-Hee; Samal, Siba K

2010-03-01

Avian metapneumovirus (AMPV) causes an upper respiratory tract infection in turkeys leading to serious economic losses to the turkey industry. The G glycoprotein of AMPV is known to be associated with viral attachment and pathogenesis. In this study, we determined the role of the G glycoprotein in the pathogenicity and immunogenicity of AMPV strain Colorado (AMPV/CO). Recombinant AMPV/CO lacking the G protein (rAMPV/CO-deltaG) was generated using a reverse-genetics system. The recovered rAMPV/CO-deltaG replicated slightly better than did wild-type AMPV in Vero cells. However, deletion of the G gene in AMPV resulted in attenuation of the virus in turkeys. The mutant virus induced less-severe clinical signs and a weaker immune response in turkeys than did the wild-type AMPV. Our results suggest that the G glycoprotein is an important determinant for the pathogenicity and immunogenicity of AMPV.

Podoplanin - a small glycoprotein with many faces

OpenAIRE

Ugorski, Maciej; Dziegiel, Piotr; Suchanski, Jaroslaw

2016-01-01

Podoplanin is a small membrane glycoprotein with a large number of O-glycoside chains and therefore it belongs to mucin-type proteins. It can be found on the surface of many types of normal cells originating from various germ layers. It is present primarily on the endothelium of lymphatic vessels, type I pneumocytes and glomerular podocytes. Increased levels of podoplanin or its neo-expression have been found in numerous types of human carcinomas, but it is especially common in squamous cell ...

[Research progress on ebola virus glycoprotein].

Science.gov (United States)

Ding, Guo-Yong; Wang, Zhi-Yu; Gao, Lu; Jiang, Bao-Fa

2013-03-01

Ebola virus (EBOV) causes outbreaks of a highly lethal hemorrhagic fever in humans and there are no effective therapeutic or prophylactic treatments available. The glycoprotein (GP) of EBOV is a transmembrane envelope protein known to play multiple functions including virus attachment and entry, cell rounding and cytotoxicity, down-regulation of host surface proteins, and enhancement of virus assembly and budding. GP is the primary target of protective immunity and the key target for developing neutralizing antibodies. In this paper, the research progress on genetic structure, pathogenesis and immunogenicity of EBOV GP in the last 5 years is reviewed.

Phosphorylation of varicella-zoster virus glycoprotein gpI by mammalian casein kinase II and casein kinase I

International Nuclear Information System (INIS)

Grose, C.; Jackson, W.; Traugh, J.A.

1989-01-01

Varicella-zoster virus (VZV) glycoprotein gpI is the predominant viral glycoprotein within the plasma membranes of infected cells. This viral glycoprotein is phosphorylated on its polypeptide backbone during biosynthesis. In this report, the authors investigated the protein kinases which participate in the phosphorylation events. Under in vivo conditions, VZV gpI was phosphorylated on its serine and threonine residues by protein kinases present within lysates of either VZV-infected or uninfected cells. Because this activity was diminished by heparin, a known inhibitor of casein kinase II, isolated gpI was incubated with purified casein kinase II and shown to be phosphorylated in an in vitro assay containing [γ- 32 P]ATP. The same glycoprotein was phosphorylated when [ 32 P]GTP was substituted for [ 32 P]ATP in the protein kinase assay. They also tested whether VZV gpI was phosphorylated by two other ubiquitous mammalian protein kinases--casein kinase I and cyclic AMP-dependent kinase--and found that only casein kinase I modified gpI. When the predicted 623-amino-acid sequence of gpI was examined, two phosphorylation sites known to be optimal for casein kinase II were observed. In summary, this study showed that VZV gpI was phosphorylated by each of two mammalian protein kinases (casein kinase I and casein kinase II) and that potential serine-threonine phosphorylation sites for each of these two kinases were present in the viral glycoprotein

Urinary excretion of beta 2-glycoprotein-1 (apolipoprotein H) and other markers of tubular malfunction in "non-tubular" renal disease.

Science.gov (United States)

Flynn, F V; Lapsley, M; Sansom, P A; Cohen, S L

1992-07-01

To determine whether urinary beta 2-glycoprotein-1 assays can provide improved discrimination between chronic renal diseases which are primarily of tubular or glomerular origin. Urinary beta 2-glycoprotein-1, retinol-binding protein, alpha 1-microglobulin, beta 2-microglobulin, N-acetyl-beta-D-glucosa-minidase and albumin were measured in 51 patients with primary glomerular disease, 23 with obstructive nephropathy, and 15 with polycystic kidney disease, and expressed per mmol of creatinine. Plasma beta 2-glycoprotein-1 was assayed in 52 patients and plasma creatinine in all 89. The findings were compared between the diagnostic groups and with previously published data relating to primary tubular disorders. All 31 patients with plasma creatinine greater than 200 mumol/l excreted increased amounts of beta 2-glycoprotein-1, retinol-binding protein, and alpha 1-microglobulin, and 29 had increased N-acetyl-beta-D-glucosaminidase; the quantities were generally similar to those found in comparable patients with primary tubular pathology. Among 58 with plasma creatinine concentrations under 200 mumol/l, increases in beta 2-glycoprotein-1, retinol-binding protein, and alpha 1-microglobulin excretion were less common and much smaller, especially in those with obstructive nephropathy and polycystic disease. The ratios of the excretion of albumin to the other proteins provided the clearest discrimination between the patients with glomerular or tubular malfunction, but an area of overlap was present which embraced those with obstructive nephropathy and polycystic disease. Increased excretion of beta 2-glycoprotein-1 due to a raised plasma concentration or diminution of tubular reabsorption, or both, is common in all the forms of renal disease investigated, and both plasma creatinine and urinary albumin must be taken into account when interpreting results. Ratios of urinary albumin: beta 2-glycoprotein-1 greater than 1000 are highly suggestive of primary glomerular disease and

A sheep hydatid cyst glycoprotein as receptors for three toxic lectins, as well as Abrus precatorius and Ricinus communis agglutinins.

Science.gov (United States)

Wu, A M; Song, S C; Wu, J H; Pfüller, U; Chow, L P; Lin, J Y

1995-01-18

The binding properties of a glycoprotein with blood group P1 specificity isolated from sheep hydatid cyst fluid with Gal and GalNAc specific lectins was investigated by quantitative precipitin and precipitin inhibition assays. The glycoprotein completely precipitated Ricinus communis agglutinin (RCA1), Abrus precatorius agglutinin (APA) and Mistletoe toxic lectin-I (ML-I). Only 1.0 microgram of P1 glycoprotein was required to precipitate 50% of 5.1 micrograms ML-I nitrogen. It also reacted well with abrin-a and ricin, precipitating over 73% of the lectin nitrogen added, but poorly or weakly with Dolichos biflorus (DBL), Vicia villosa (VVL, a mixture of A4, A2B2 and B4), VVL-B4, Arachis hypogaea (PNA), Maclura pomifera (MPL), Bauchinia purpurea alba (BPL) and Wistaria floribunda (WFL) lectins. When an inhibition assay in the range of 5.1 micrograms N to 5.9 micrograms N of lectins (ML-I, abrin-a; ricin, RCA1, and APA, and 10 micrograms P1 active glycoprotein interaction was performed; from 76 to 100% of the precipitations were inhibited by 0.44 and 0.52 mumol of Gal alpha 1-->4Gal and Gal beta 1-->4GlcNAc, respectively, but not or insignificantly with 1.72 mumol of GlcNAc. The Gal alpha 1-->4Gal disaccharide found in this P1 active glycoprotein is a frequently occurring sequence of many glycosphingolipids located at the surface of mammalian cell membranes, especially human erythrocytes and intestinal cells for ligand binding and microbial toxin attachment. The present finding suggests that the Gal alpha 1-->4Gal beta 1-->4GlcNAc sequence in this P1 active glycoprotein is one of the best glycoprotein receptors for three toxic lectins (ricin, abrin-a, and ML-I) as well as for APA, and RCA1, and the result of inhibition assay implies that these lectins are recognizing part or all of the Gal alpha 1-->4Gal beta 1-->4GlcNAc sequence in the P1 active glycoprotein.

Influence of sea ice cover and icebergs on circulation and water mass formation in a numerical circulation model of the Ross Sea, Antarctica

Science.gov (United States)

Dinniman, Michael S.; Klinck, John M.; Smith, Walker O.

2007-11-01

Satellite imagery shows that there was substantial variability in the sea ice extent in the Ross Sea during 2001-2003. Much of this variability is thought to be due to several large icebergs that moved through the area during that period. The effects of these changes in sea ice on circulation and water mass distributions are investigated with a numerical general circulation model. It would be difficult to simulate the highly variable sea ice from 2001 to 2003 with a dynamic sea ice model since much of the variability was due to the floating icebergs. Here, sea ice concentration is specified from satellite observations. To examine the effects of changes in sea ice due to iceberg C-19, simulations were performed using either climatological ice concentrations or the observed ice for that period. The heat balance around the Ross Sea Polynya (RSP) shows that the dominant term in the surface heat budget is the net exchange with the atmosphere, but advection of oceanic warm water is also important. The area average annual basal melt rate beneath the Ross Ice Shelf is reduced by 12% in the observed sea ice simulation. The observed sea ice simulation also creates more High-Salinity Shelf Water. Another simulation was performed with observed sea ice and a fixed iceberg representing B-15A. There is reduced advection of warm surface water during summer from the RSP into McMurdo Sound due to B-15A, but a much stronger reduction is due to the late opening of the RSP in early 2003 because of C-19.

William Parsons, 3rd Earl of Rosse astronomy and the castle in nineteenth-century Ireland

CERN Document Server

Mollan, R Charles

2015-01-01

This is a revealing account of the family life and achievements of the Third Earl of Rosse, a hereditary peer and resident landlord at Birr Castle, County Offaly, in nineteenth-century Ireland, before, during and after the devastating famine of the 1840s. He was a remarkable engineer, who built enormous telescopes in the cloudy middle of Ireland. The book gives details, in an attractive non-technical style which requires no previous scientific knowledge, of his engineering initiatives and the astronomical results, but also reveals much more about the man and his contributions - locally in the

Identification of a mouse synaptic glycoprotein gene in cultured neurons.

Science.gov (United States)

Yu, Albert Cheung-Hoi; Sun, Chun Xiao; Li, Qiang; Liu, Hua Dong; Wang, Chen Ran; Zhao, Guo Ping; Jin, Meilei; Lau, Lok Ting; Fung, Yin-Wan Wendy; Liu, Shuang

2005-10-01

Neuronal differentiation and aging are known to involve many genes, which may also be differentially expressed during these developmental processes. From primary cultured cerebral cortical neurons, we have previously identified various differentially expressed gene transcripts from cultured cortical neurons using the technique of arbitrarily primed PCR (RAP-PCR). Among these transcripts, clone 0-2 was found to have high homology to rat and human synaptic glycoprotein. By in silico analysis using an EST database and the FACTURA software, the full-length sequence of 0-2 was assembled and the clone was named as mouse synaptic glycoprotein homolog 2 (mSC2). DNA sequencing revealed transcript size of mSC2 being smaller than the human and rat homologs. RT-PCR indicated that mSC2 was expressed differentially at various culture days. The mSC2 gene was located in various tissues with higher expression in brain, lung, and liver. Functions of mSC2 in neurons and other tissues remain elusive and will require more investigation.

Provenance and post-depositional low-temperature evolution of the James Ross Basin sedimentary rocks (Antarctic Peninsula) based on fission track analysis

Czech Academy of Sciences Publication Activity Database

Svojtka, Martin; Nývlt, D.; Murakami, Masaki; Vávrová, J.; Filip, Jiří; Mixa, P.

2009-01-01

Roč. 21, č. 6 (2009), s. 593-607 ISSN 0954-1020 R&D Projects: GA MŠk 1K05030 Institutional research plan: CEZ:AV0Z30130516 Keywords : James Ross Island * Seymour Island * Cretaceous–Palaeogene succession * fission track dating * zircon * apatite Subject RIV: DB - Geology ; Mineralogy Impact factor: 1.496, year: 2009

Intercellular transfer of P-glycoprotein from the drug resistant human bladder cancer cell line BIU-87 does not require cell-to-cell contact.

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Zhou, Hui-liang; Zheng, Yong-jun; Cheng, Xiao-zhi; Lv, Yi-song; Gao, Rui; Mao, Hou-ping; Chen, Qin

2013-09-01

The efflux activity of transmembrane P-glycoprotein prevents various therapeutic drugs from reaching lethal concentrations in cancer cells, resulting in multidrug resistance. We investigated whether drug resistant bladder cancer cells could transfer functional P-glycoprotein to sensitive parental cells. Drug sensitive BIU-87 bladder cancer cells were co-cultured for 48 hours with BIU-87/ADM, a doxorubicin resistant derivative of the same cell line, in a Transwell® system that prevented cell-to-cell contact. The presence of P-glycoprotein in recipient cell membranes was established using fluorescein isothiocyanate, laser scanning confocal microscopy and Western blot. P-glycoprotein mRNA levels were compared between cell types. Rhodamine 123 efflux assay was done to confirm that P-glycoprotein was biologically active. The amount of P-glycoprotein protein in BIU-87 cells co-cultured with BIU-87/ADM was significantly higher than in BIU-87 cells (0.44 vs 0.25) and BIU-87/H33342 cells (0.44 vs 0.26, each p transfer. P-glycoprotein mRNA expression was significantly higher in BIU-87/ADM cells than in co-cultured BIU-87 cells (1.28 vs 0.30), BIU-87/H33342 (0.28) and BIU-87 cells (0.25, each p <0.001), ruling out a genetic mechanism. After 30 minutes of efflux, rhodamine 123 fluorescence intensity was significantly lower in BIU-87/ADM cells (5.55 vs 51.45, p = 0.004) and co-cultured BIU-87 cells than in BIU-87 cells (14.22 vs 51.45, p <0.001), indicating that P-glycoprotein was functional. Bladder cancer cells can acquire functional P-glycoprotein through a nongenetic mechanism that does not require direct cell contact. This mechanism is consistent with a microparticle mediated process. Copyright © 2013 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS

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Wang Bin

2007-12-01

Full Text Available Abstract Background CCR5-restricted (R5 human immunodeficiency virus type 1 (HIV-1 variants cause CD4+ T-cell loss in the majority of individuals who progress to AIDS, but mechanisms underlying the pathogenicity of R5 strains are poorly understood. To better understand envelope glycoprotein (Env determinants contributing to pathogenicity of R5 viruses, we characterized 37 full-length R5 Envs from cross-sectional and longitudinal R5 viruses isolated from blood of patients with asymptomatic infection or AIDS, referred to as pre-AIDS (PA and AIDS (A R5 Envs, respectively. Results Compared to PA-R5 Envs, A-R5 Envs had enhanced fusogenicity in quantitative cell-cell fusion assays, and reduced sensitivity to inhibition by the fusion inhibitor T-20. Sequence analysis identified the presence of Asn 362 (N362, a potential N-linked glycosylation site immediately N-terminal to CD4-binding site (CD4bs residues in the C3 region of gp120, more frequently in A-R5 Envs than PA-R5 Envs. N362 was associated with enhanced fusogenicity, faster entry kinetics, and increased sensitivity of Env-pseudotyped reporter viruses to neutralization by the CD4bs-directed Env mAb IgG1b12. Mutagenesis studies showed N362 contributes to enhanced fusogenicity of most A-R5 Envs. Molecular models indicate N362 is located adjacent to the CD4 binding loop of gp120, and suggest N362 may enhance fusogenicity by promoting greater exposure of the CD4bs and/or stabilizing the CD4-bound Env structure. Conclusion Enhanced fusogenicity is a phenotype of the A-R5 Envs studied, which was associated with the presence of N362, enhanced HIV-1 entry kinetics and increased CD4bs exposure in gp120. N362 contributes to fusogenicity of R5 Envs in a strain dependent manner. Our studies suggest enhanced fusogenicity of A-R5 Envs may contribute to CD4+ T-cell loss in subjects who progress to AIDS whilst harbouring R5 HIV-1 variants. N362 may contribute to this effect in some individuals.

An unusual dependence of human herpesvirus-8 Glycoproteins-induced cell-to-cell fusion on heparan sulfate

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Tiwari, Vaibhav; Darmani, Nissar A.; Thrush, Gerald R.; Shukla, Deepak

2009-01-01

Human herpes virus 8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from following lines of evidence: 1) a significant reduction in cell-to-cell fusion occurred when target cells were treated with heparinase; 2) in a competition assay, when the effector cells expressing HHV-8 glycoproteins were challenged with soluble HS, cell-to-cell fusion was reduced; and, 3) coexpression of HHV-8 glycoproteins gH-gL on target cells resulted in inhibition of cell surface HS expression. Taken together, our results indicate that cell surface HS can play an additional role during HHV-8 pathogenesis. PMID:19747451

Comparative Analysis of Whey N-Glycoproteins in Human Colostrum and Mature Milk Using Quantitative Glycoproteomics.

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Cao, Xueyan; Song, Dahe; Yang, Mei; Yang, Ning; Ye, Qing; Tao, Dongbing; Liu, Biao; Wu, Rina; Yue, Xiqing

2017-11-29

Glycosylation is a ubiquitous post-translational protein modification that plays a substantial role in various processes. However, whey glycoproteins in human milk have not been completely profiled. Herein, we used quantitative glycoproteomics to quantify whey N-glycosylation sites and their alteration in human milk during lactation; 110 N-glycosylation sites on 63 proteins and 91 N-glycosylation sites on 53 proteins were quantified in colostrum and mature milk whey, respectively. Among these, 68 glycosylation sites on 38 proteins were differentially expressed in human colostrum and mature milk whey. These differentially expressed N-glycoproteins were highly enriched in "localization", "extracellular region part", and "modified amino acid binding" according to gene ontology annotation and mainly involved in complement and coagulation cascades pathway. These results shed light on the glycosylation sites, composition and biological functions of whey N-glycoproteins in human colostrum and mature milk, and provide substantial insight into the role of protein glycosylation during infant development.

Molecular cloning of S1 glycoprotein gene of infectious bronchitis ...

African Journals Online (AJOL)

In vitro protein expression is an important method of obtaining large amounts of viral proteins to investigate their biological properties. The S1 glycoprotein of infectious bronchitis virus, due to its effective immune-dominant role is an appropriate candidate for production of recombinant vaccine against infectious bronchitis ...

The Lyssavirus glycoprotein: A key to cross-immunity

International Nuclear Information System (INIS)

Buthelezi, Sindisiwe G.; Dirr, Heini W.; Chakauya, Ereck; Chikwamba, Rachel; Martens, Lennart; Tsekoa, Tsepo L.; Stoychev, Stoyan H.; Vandermarliere, Elien

2016-01-01

Rabies is an acute viral encephalomyelitis in warm-blooded vertebrates, caused by viruses belonging to Rhabdovirus family and genus Lyssavirus. Although rabies is categorised as a neglected disease, the rabies virus (RABV) is the most studied amongst Lyssaviruses which show nearly identical infection patterns. In efforts to improving post-exposure prophylaxis, several anti-rabies monoclonal antibodies (mAbs) targeting the glycoprotein (G protein) sites I, II, III and G5 have been characterized. To explore cross-neutralization capacity of available mAbs and discover new possible B-cell epitopes, we have analyzed all available glycoprotein sequences from Lyssaviruses with a focus on sequence variation and conservation. This information was mapped on the structure of a representative G protein. We proposed several possible cross-neutralizing B-cell epitopes (GUVTTTF, WLRTV, REECLD and EHLVVEEL) in complement to the already well-characterized antigenic sites. The research could facilitate development of novel cross-reactive mAbs against RABV and even more broad, against possibly all Lyssavirus members. -- Highlights: •The current PEP has raised safety and availability concerns. •Cocktails of mAbs have been proposed as alternative treatment. •Amino acid conservation amongst Lyssavirus G proteins was studied. •Possible cross-neutralizing B-cell epitopes were proposed.

The Lyssavirus glycoprotein: A key to cross-immunity

Energy Technology Data Exchange (ETDEWEB)

Buthelezi, Sindisiwe G. [Council for Scientific and Industrial Research, Biosciences Unit, Pretoria (South Africa); Protein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg (South Africa); Dirr, Heini W. [Protein Structure-Function Research Unit, School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg (South Africa); Chakauya, Ereck; Chikwamba, Rachel [Council for Scientific and Industrial Research, Biosciences Unit, Pretoria (South Africa); Martens, Lennart [Unit for Computational Omics and Systems Biology, Medical Biotechnology Center, VIB, Ghent (Belgium); Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Ghent (Belgium); Tsekoa, Tsepo L. [Council for Scientific and Industrial Research, Biosciences Unit, Pretoria (South Africa); Stoychev, Stoyan H., E-mail: Sstoychev@csir.co.za [Council for Scientific and Industrial Research, Biosciences Unit, Pretoria (South Africa); Vandermarliere, Elien [Unit for Computational Omics and Systems Biology, Medical Biotechnology Center, VIB, Ghent (Belgium); Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Ghent (Belgium); Bioinformatics Institute Gent, Ghent University, Ghent (Belgium)

2016-11-15

Rabies is an acute viral encephalomyelitis in warm-blooded vertebrates, caused by viruses belonging to Rhabdovirus family and genus Lyssavirus. Although rabies is categorised as a neglected disease, the rabies virus (RABV) is the most studied amongst Lyssaviruses which show nearly identical infection patterns. In efforts to improving post-exposure prophylaxis, several anti-rabies monoclonal antibodies (mAbs) targeting the glycoprotein (G protein) sites I, II, III and G5 have been characterized. To explore cross-neutralization capacity of available mAbs and discover new possible B-cell epitopes, we have analyzed all available glycoprotein sequences from Lyssaviruses with a focus on sequence variation and conservation. This information was mapped on the structure of a representative G protein. We proposed several possible cross-neutralizing B-cell epitopes (GUVTTTF, WLRTV, REECLD and EHLVVEEL) in complement to the already well-characterized antigenic sites. The research could facilitate development of novel cross-reactive mAbs against RABV and even more broad, against possibly all Lyssavirus members. -- Highlights: •The current PEP has raised safety and availability concerns. •Cocktails of mAbs have been proposed as alternative treatment. •Amino acid conservation amongst Lyssavirus G proteins was studied. •Possible cross-neutralizing B-cell epitopes were proposed.

Glycoprotein profiles of macrophages at different stages of activation as revealed by lectin binding after electrophoretic separation.

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Irimura, T; North, S M; Nicolson, G L

1987-01-01

Glycoprotein profiles of rat macrophages (M phi) at different stages of activation were studied by examining the reactivity of various lectins to the glycoproteins separated by polyacrylamide gel electrophoresis. Ricinus communis agglutinin 1 (RCA1) revealed several components including glycoproteins of Mr 160 kDa and 65 kDa prominent in resident M phi. A pokeweed mitogen (PWM) isolectin, Pa-4, recognizes branched poly(N-acetyllactosamine)-type carbohydrate chains, and revealed a significant increase in glycoproteins of Mr ranging from 70 kDa to 150 kDa on thioglycolate-elicited M phi. Increased reactivity of PWM to thioglycolate-elicited M phi was observed by direct binding of 125I-labeled Pa-4 to intact or glutaraldehyde-fixed M phi. Histochemical staining of formaldehyde-fixed M phi in vitro with biotinylated Pa-4 was consistent with the gel analysis, that is, resident M phi had no reactivity while thioglycolate-elicited M phi showed slight reactivity. Alveolar and intratumoral M phi bound more Pa-4 than resident or thioglycolate-elicited M phi. The PWM isolectin may therefore serve as a marker for an early stage of M phi activation.

N-terminal sequence of human leukocyte glycoprotein Mo1: conservation across species and homology to platelet IIb/IIIa.

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Pierce, M W; Remold-O'Donnell, E; Todd, R F; Arnaout, M A

1986-12-12

Mo1 and gp160-gp93 are two surface membrane glycoprotein heterodimers present on granulocytes and monocytes derived from humans and guinea pigs, respectively. We purified both antigens and found that their alpha subunits had identical N-termini which were significantly homologous to the alpha subunit of the human adhesion platelet glycoprotein IIb/IIIa.

The Ross Procedure in Patients among the Pediatric Population, Post Ten Years of Experience

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Andrey A. Ivanov

2013-09-01

Full Text Available The aim of the study was to analyze the results of the surgical treatment in pediatric patients who had undergone the Ross procedure.Material and Methods: The study involved 114 patients between 12 days to 18 years in age. The early and late (up to 5 years results of the treatment were studied. The examination included echocardiography, catheterization of the cardiac chambers and angiocardiography. The case distribution of patients based on diagnosis was as follows: isolated aortic valve stenosis (IAVS in 38 (33.3% patients, aortic valve insufficiency (AVI in 33 (28.9%, and combined heart defects in 56 patients (49.1%.Results: The death rate was 6.14% during the early postoperative period and 1.14% in the late postoperative period; the actuarial survival in the long-term was 98.86%. The complication rate was 51.5%. The most frequent complication was pericarditis (25.6%, whereas cardiac and respiratory failure occurred in 7.6% of the cases and cardiac arrhythmias in 6.1% of the cases. The average time spent in the intensive care unit was 3.48 ± 2.90 days; the hospitalization period on average was 24.70±10.87 days. After surgery, there was a tendency of the echocardiographic parameters to move toward normalization. The frequency of reoperation in the late period was 23.7%, the main reason for which being the conduit dysfunction in the position of the pulmonary artery (PA.Conclusion: The clinical efficacy of the Ross procedure in the treatment of aortic valve malformations in the pediatric group was confirmed. However, in some cases, the need to perform repeated operations due to the increase in the ring size and an increase in the neo-aortic insufficiency during the somatic growth process.

Evaluating the Effects of Cytomegalovirus Glycoprotein B on the Maturation and Function of Monocyte-derived dendritic cells

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Afsson shariat

2015-11-01

Full Text Available Background & Objectives: Interaction of cytomegalovirus glycoprotein B with toll-like receptors of dendritic cells leads to early signaling and innate immune responses. The aim of this study is to evaluate the effects of cytomegalovirus glycoprotein B on the maturation and function of monocyte-derived dendritic cells in treated groups in comparison with control groups. Materials & Methods: Blood samples were taken from 5 healthy volunteers. Following the generation of monocyte-derived dendritic cells on the fifth day of cell culture, half of the immature dendritic cells were treated with cytomegalovirus glycoprotein B, and the rest of them were induced to mature dendritic untreated cells and were used as the control group. The maturation and function of dendritic cells were evaluated in these two groups. Results: The gene expression level of toll-like receptor-4 significantly increased in the group treated with glycoprotein B (p < 0.05, whereas there were no significant differences in the expression rates of CD83, CD86, CD1a, and HLA-DR and the secretion of IL-23 from monocyte-derived dendritic cells between the treated groups and the controls. Conclusion: The increase in the gene expression of toll-like receptor-4 in monocyte-derived dendritic cells treated with cytomegalovirus glycoprotein B showed that cell contact is required to elicit cellular antiviral response and toll-like receptor activation. Thus, it is critical to recognize the viral and cellular determinants of the immune system in order to develop new therapeutic strategies against cytomegalovirus.