Suured prügifirmad püüavad Saaremaa turgu / Mehis Tulk

Index Scriptorium Estoniae

Tulk, Mehis, 1967-

2004-01-01

Eelseisvate jäätmeveokonkursside tarvis on Saare maakonnas jäätmeloa hankinud lisaks OÜ-le Prügimees ka suured välismaised firmad Ragn-Sells ja Cleanaway. Tabel: Andmed jäätmekäitlusfirmade OÜ Prügimees, Cleanaway, Ragn Sells kohta. Vt. samas: Luba igal juhul vajalik

A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25

DEFF Research Database (Denmark)

Douthwaite, S; Garrett, R A; Wagner, R

1979-01-01

An RNA fragment, constituting three subfragments of nucleotide sequences 1-11, 69-87 and 89-120, is the most ribonuclease-resistant part of the native 5S RNA of Escherichia coli, at 0 degrees C. A smaller fragment of nucleotide sequence 69-87 and 90-110 is ribonuclease-resistant at 25 degrees....... Degradation of the L25-5S RNA complex with ribonuclease A or T2 yielded RNA fragments similar to those of the free 5S RNA at 0 degrees C and 25 degrees C; moreover L25 remained strongly bound to both RNA fragments and also produced some opening of the RNA structure in at least two positions. Protein L18...... initially protected most of the 5S RNA against ribonuclease digestion, at 0 degrees C, but was then gradually released prior to the formation of the larger RNA fragment. It cannot be concluded, therefore, as it was earlier (Gray et al., 1973), that this RNA fragment contains the primary binding site of L18....

Sequence analysis of L RNA of Lassa virus

International Nuclear Information System (INIS)

Vieth, Simon; Torda, Andrew E.; Asper, Marcel; Schmitz, Herbert; Guenther, Stephan

2004-01-01

The L RNA of three Lassa virus strains originating from Nigeria, Ghana/Ivory Coast, and Sierra Leone was sequenced and the data subjected to structure predictions and phylogenetic analyses. The L gene products had 2218-2221 residues, diverged by 18% at the amino acid level, and contained several conserved regions. Only one region of 504 residues (positions 1043-1546) could be assigned a function, namely that of an RNA polymerase. Secondary structure predictions suggest that this domain is very similar to RNA-dependent RNA polymerases of known structure encoded by plus-strand RNA viruses, permitting a model to be built. Outside the polymerase region, there is little structural data, except for regions of strong alpha-helical content and probably a coiled-coil domain at the N terminus. No evidence for reassortment or recombination during Lassa virus evolution was found. The secondary structure-assisted alignment of the RNA polymerase region permitted a reliable reconstruction of the phylogeny of all negative-strand RNA viruses, indicating that Arenaviridae are most closely related to Nairoviruses. In conclusion, the data provide a basis for structural and functional characterization of the Lassa virus L protein and reveal new insights into the phylogeny of negative-strand RNA viruses

Kümme teatristarti alustas suureks kasvamisega / Tiiu Laks

Index Scriptorium Estoniae

Laks, Tiiu,1984-

2010-01-01

Tallinna Linnateater annab kümnele algajale võimaluse lavastada professionaalses teatris. 6. oktoobril esietendunud monotükist "Suur mees juba", millega jõudis lavale kolm professionaalset debüüti korraga - Kaarel B. Väljamäe esimene teatritekst, Diana Leesalu esimene lavastus ning Priit Piusi esimene suur roll

Synthesis and Structural Characterization of 2'-Fluoro-α-L-RNA-Modified Oligonucleotides

DEFF Research Database (Denmark)

Bundgaard Jensen, Troels; Pasternak, Anna; Stahl Madsen, Andreas

2011-01-01

with the smallest destabilization towards RNA. Thermodynamic data show that the duplex formation with 2'-fluoro-α-L-RNA nucleotides is enthalpically disfavored but entropically favored. 2'-Fluoro-α-L-RNA nucleotides exhibit very good base pairing specificity following Watson-Crick rules. The 2'-fluoro......-α-L-RNA monomer was designed as a monocyclic mimic of the bicyclic α-L-LNA, and molecular modeling showed that this indeed is the case as the 2'-fluoro monomer adopts a C3'-endo/C2'-exo sugar pucker. Molecular modeling of modified duplexes show that the 2'-fluoro-α-L-RNA nucleotides partake in Watson-Crick base......We describe the synthesis and binding properties of oligonucleotides that contain one or more 2'-fluoro-α-L-RNA thymine monomer(s). Incorporation of 2'-fluoro-α-L-RNA thymine into oligodeoxynucleotides decreased thermal binding stability slightly upon hybridization with complementary DNA and RNA...

Retarding effect of prior-overloading on stress corrosion cracking of cold rolled 316L SS in simulated PWR water environment

Science.gov (United States)

Chen, Junjie; Lu, Zhanpeng; Xiao, Qian; Ru, Xiangkun; Ma, Jiarong; Shoji, Tetsuo

2017-12-01

The effect of prior single tensile overloading on the stress corrosion cracking behavior of cold rolled 316L in a simulated PWR water environment at 310 °C was investigated. SCC growth retardation by overloading was observed in cold rolled 316L specimens in both the T-L and L-T orientations. The stretch zone observed on the fracture surfaces of the overloaded specimens affected SCC propagation. The compressive residual stress induced by overloading process reduced the effective driving force of SCC propagation. The negative dK/da effect ahead of the crack tip likely contributes to the retardation of SCC growth. The duration of overloading is dependent on water chemistry and the local stress conditions.

Lauli mi, tuvi' toro sisse, kalli kramahvoona sisse... ehk Neiu surm & suured saajad / Marju Torp-Kõivupuu

Index Scriptorium Estoniae

Torp-Kõivupuu, Marju, 1960-

2004-01-01

Rets. rmt.: The Maiden's Death Song & The Great Wedding : Anne Vabarna's oral twin epic written down by A. O. Väisanen / by Lauri Honko in collaboration with Anneli Honko and Paul Hagu. Helsinki, 2003. Setu rahvalauliku Anne Vabarna kaksikeeposest "Suured saajad. Neiu koolulaul"

Põhja-Korea tuumakriisis saavutati läbimurre / Kalev Kask

Index Scriptorium Estoniae

Kask, Kalev

2007-01-01

Kuuepoolsel läbirääkimisel sõlmitud leppe järgi lubab Põhja-Korea sulgeda vaidlusaluse Youngbyoni tuumajaama, kus valmistatakse väidetavalt plutooniumi, peatingimuseks saada vastutasuks suures koguses elektrienergiat

Detailed analysis of RNA-protein interactions within the bacterial ribosomal protein L5/5S rRNA complex.

Science.gov (United States)

Perederina, Anna; Nevskaya, Natalia; Nikonov, Oleg; Nikulin, Alexei; Dumas, Philippe; Yao, Min; Tanaka, Isao; Garber, Maria; Gongadze, George; Nikonov, Stanislav

2002-12-01

The crystal structure of ribosomal protein L5 from Thermus thermophilus complexed with a 34-nt fragment comprising helix III and loop C of Escherichia coli 5S rRNA has been determined at 2.5 A resolution. The protein specifically interacts with the bulged nucleotides at the top of loop C of 5S rRNA. The rRNA and protein contact surfaces are strongly stabilized by intramolecular interactions. Charged and polar atoms forming the network of conserved intermolecular hydrogen bonds are located in two narrow planar parallel layers belonging to the protein and rRNA, respectively. The regions, including these atoms conserved in Bacteria and Archaea, can be considered an RNA-protein recognition module. Comparison of the T. thermophilus L5 structure in the RNA-bound form with the isolated Bacillus stearothermophilus L5 structure shows that the RNA-recognition module on the protein surface does not undergo significant changes upon RNA binding. In the crystal of the complex, the protein interacts with another RNA molecule in the asymmetric unit through the beta-sheet concave surface. This protein/RNA interface simulates the interaction of L5 with 23S rRNA observed in the Haloarcula marismortui 50S ribosomal subunit.

16.-17. sajandi kirikukalmistu luustike antropoloogiline analüüs (Tallinn Suur-Kloostri 14) / Raili Allmäe

Index Scriptorium Estoniae

Allmäe, Raili, 1963-

2008-01-01

Tallinna Issanda Muutmise kiriku territooriumil 2003. aastal toimunud arheoloogilistest kaevamistest. Peamiselt 16.-17. sajandi kalmistul fikseeriti 144 matust. Laste matuste suur osakaal matuste üldhulgast võib viidata kiriku juures tegutsenud õppeasutusele või laste hoolekandeasutusele

Miks ekspordib Hiina Venemaale läbi Tallinna? / interv. Mihkel Kärmas

Index Scriptorium Estoniae

2005-01-01

Hiina suured kaubalennukid veavad Venemaale kaupu läbi Tallinna lennujaama. Lühiintervjuu Tallinna tolliinspektuuri kontrolltalituse juhataja Peeter Kõve, lennujaama turundusdirektori Eduard Tüüri ja lennuameti asedirektori Rein Porroga

Juhan Parts : oleme Isamaaliiduga lähedased / Juhan Parts ; interv. Toomas Mattson

Index Scriptorium Estoniae

Parts, Juhan, 1966-

2003-01-01

Peaminister Juhan Parts vastab küsimustele, mis puudutavad Isamaaliitu kui Res Publicale kõige lähedasemat võimalikku koalitsioonipartnerit, nn suure koalitsiooni tekkimise võimalust. Skeem: Jõujooned Riigikogus

Finite-element modeling of soft tissue rolling indentation.

Science.gov (United States)

Sangpradit, Kiattisak; Liu, Hongbin; Dasgupta, Prokar; Althoefer, Kaspar; Seneviratne, Lakmal D

2011-12-01

We describe a finite-element (FE) model for simulating wheel-rolling tissue deformations using a rolling FE model (RFEM). A wheeled probe performing rolling tissue indentation has proven to be a promising approach for compensating for the loss of haptic and tactile feedback experienced during robotic-assisted minimally invasive surgery (H. Liu, D. P. Noonan, B. J. Challacombe, P. Dasgupta, L. D. Seneviratne, and K. Althoefer, "Rolling mechanical imaging for tissue abnormality localization during minimally invasive surgery, " IEEE Trans. Biomed. Eng., vol. 57, no. 2, pp. 404-414, Feb. 2010; K. Sangpradit, H. Liu, L. Seneviratne, and K. Althoefer, "Tissue identification using inverse finite element analysis of rolling indentation," in Proc. IEEE Int. Conf. Robot. Autom. , Kobe, Japan, 2009, pp. 1250-1255; H. Liu, D. Noonan, K. Althoefer, and L. Seneviratne, "The rolling approach for soft tissue modeling and mechanical imaging during robot-assisted minimally invasive surgery," in Proc. IEEE Int. Conf. Robot. Autom., May 2008, pp. 845-850; H. Liu, P. Puangmali, D. Zbyszewski, O. Elhage, P. Dasgupta, J. S. Dai, L. Seneviratne, and K. Althoefer, "An indentation depth-force sensing wheeled probe for abnormality identification during minimally invasive surgery," Proc. Inst. Mech. Eng., H, vol. 224, no. 6, pp. 751-63, 2010; D. Noonan, H. Liu, Y. Zweiri, K. Althoefer, and L. Seneviratne, "A dual-function wheeled probe for tissue viscoelastic property identification during minimally invasive surgery," in Proc. IEEE Int. Conf. Robot. Autom. , 2008, pp. 2629-2634; H. Liu, J. Li, Q. I. Poon, L. D. Seneviratne, and K. Althoefer, "Miniaturized force indentation-depth sensor for tissue abnormality identification," IEEE Int. Conf. Robot. Autom., May 2010, pp. 3654-3659). A sound understanding of wheel-tissue rolling interaction dynamics will facilitate the evaluation of signals from rolling indentation. In this paper, we model the dynamic interactions between a wheeled probe and a

Sisaliku jäljed piiblitõlkes : lõunaeestikeelse Vana Testamendi saatusest / Kristiina Ross

Index Scriptorium Estoniae

Ross, Kristiina, 1955-

2011-01-01

Uuritakse Kambja õpetaja Heinrich Anreas Erxlebeni lõunaeestikeelse Vana Testamendi tõlget (1796) ning leitakse, et sõnastuslikult langeb see suures osas kokku sada aastat varasema Andreas ja Adrian Virginiuse põhjaeestikeelse käsikirjaga

Study of rolled uranium annealing process; Etude du recuit de l'uranium lamine

Energy Technology Data Exchange (ETDEWEB)

Cabane, G [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1954-06-15

The dilatometric study of rolled uranium clearly shows not only the expansions or contractions induced by stress relief or diffusion of vacancies, but also the slope variations of the cooling curves, which are the best evidence of a texture change. Under the microscope, hard-rolled sheets appear as a mixture of two distinct structures; it is also possible by intermediate annealing to prepare homogeneous sheets of either structure, i.e. twinned or untwinned. All these sheets which have similar textures, undergo at first a primary recrystallization beginning at 320 deg C, then a texture change without any apparent crystal growth, at about 430 deg C. (author) [French] L'anisotropie de l'uranium {alpha} se manifeste fortement dans les coefficients de dilatation. Aussi la dilatometrie permet lle de reperer facilement les expansions ou contractions dues a des relachements de tensions ou a des disparitions de lacunes, ainsi que les variations de pente des courbes de refroidissement, qui constituent la plus importante manifestation d'un changement de texture. Au microscope, les toles fortement ecrouies apparaissent generalement formees d'un melange de deux structures differentes; on a pu aussi preparer des toles de structure homogene: les unes formees de cristaux macles, les autres apparemment depourvues de macles. Ces toles, qui ont toutes a peu pres la meme texture, subissent d'abord une recristallisation primaire a partir de 320 deg C, puis a 430 deg C environ, un changement de texture sans grossissement apparent de cristaux. (auteur)

A simulation study on the multi-pass rolling bond of 316L/Q345R stainless clad plate

Directory of Open Access Journals (Sweden)

Qin Qin

2015-07-01

Full Text Available This article describes an investigation into interface bonding research of 316L/Q345R stainless clad plate. A three-dimensional thermal–elastic–plastic model has been established using finite element analysis to model the multi-pass hot rolling process. Results of the model have been compared with those obtained from a rolling experiment of stainless clad plate. The comparisons of temperature and profile of the rolled stainless clad plate have indicated a satisfactory accuracy of finite element analysis simulation. Effects on interface bonding by different parameters including pre-heating temperature, multi-pass thickness reduction rules, rolling speed, covering rate, and different assemble patterns were analyzed systematically. The results show that higher temperature and larger thickness reduction are beneficial to achieve the bonding in vacuum hot rolling process. The critical reduction in the bond at the temperature of 1200 °C is 28%, and the critical thickness reduction reduces by about 2% when the temperature increases by 50 °C during the range from 1000 °C to 1250 °C. And the relationship between the minimum pass number and thickness reduction has been suggested. The results also indicate that large covering rate in the assemble pattern of outer soft and inner hard is beneficial to achieve the bond of stainless clad plate.

Roll forming of eco-friendly stud

Science.gov (United States)

Keum, Y. T.; Lee, S. Y.; Lee, T. H.; Sim, J. K.

2013-12-01

In order to manufacture an eco-friendly stud, the sheared pattern is designed by the Taguchi method and expanded by the side rolls. The seven geometrical shape of sheared pattern are considered in the structural and thermal analyses to select the best functional one in terms of the durability and fire resistance of dry wall. For optimizing the size of the sheared pattern chosen, the L9 orthogonal array and smaller-the-better characteristics of the Taguchi method are used. As the roll gap causes forming defects when the upper-and-lower roll type is adopted for expanding the sheared pattern, the side roll type is introduced. The stress and strain distributions obtained by the FEM simulation of roll-forming processes are utilized for the design of expanding process. The expanding process by side rolls shortens the length of expanding process and minimizes the cost of dies. Furthermore, the stud manufactured by expanding the sheared pattern of the web is an eco-friend because of the scrapless roll-forming process. In addition, compared to the conventionally roll-formed stud, the material cost is lessened about 13.6% and the weight is lightened about 15.5%.

Kirsipuu : peaaegu kindel, et lõpetan järgmisel aastal / Andres Kalvik

Index Scriptorium Estoniae

Kalvik, Andres, 1982-

2005-01-01

Pärast katkestamis Madridi MMi grupisõidus märkis Eesti parim tegevjalgrattur Jaan Kirsipuu, et suure tõenäosusega lõpeb koos Credit Agricolì lepinguga järgmisel aastal ka tema profisportlase karjäär

Suvised suured rockifestivalid / Margus Kiis

Index Scriptorium Estoniae

Kiis, Margus

2008-01-01

Lühiülevaade suvistest festivalidest: Rabarock 13. ja 14. juunil Järvakandis, Hard Rock Laager 27. ja 28. juunil Vana-Vigalas, Plink Plonk 19. juulil Tartu lauluväljaku kõrval, Punk'n Roll 22. ja 23. augustil Kilingi-Nõmmel

File list: Pol.CeL.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Pol.CeL.50.RNA_polymerase_II.AllCell dm3 RNA polymerase RNA polymerase II Cell line...70,SRX749072,SRX749071,SRX749073,SRX017852,SRX529168 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.CeL.50.RNA_polymerase_II.AllCell.bed ...

File list: Pol.CeL.20.RNA_polymerase_II.AllCell [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available Pol.CeL.20.RNA_polymerase_II.AllCell dm3 RNA polymerase RNA polymerase II Cell line...70,SRX749072,SRX749071,SRX749073,SRX017852,SRX529168 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Pol.CeL.20.RNA_polymerase_II.AllCell.bed ...

Bobby heitis Bert Pringile väljakutse / Aigi Viira

Index Scriptorium Estoniae

Viira, Aigi

2004-01-01

Laulja Bert Pringi, kelle kehastada on suur roll Smithbridge Productions uues muusikaliprojektis - George Gershwini "Hull sinu järele", lavastaja Andrus Vaarik. Ka näitleja Andero Ermeli rollist loobumise põhjustest

Suurärimees pakub Lasnamäe karjääri eest 150 miljonit krooni / Urmas Seaver

Index Scriptorium Estoniae

Seaver, Urmas, 1973-

2006-01-01

Vt. ka Postimees : na russkom jazõke 4. mai lk. 10. Suurärimehe Endel Siffi firma Primos OÜ on valmis Lasnamäe paekarjääri ala korrastama 100 miljoni krooni eest, lisaks maksaks firma linnale hoonestusõiguse eest 50 miljonit krooni. Linnavalitsus ei ole veel võtnud seisukohta, kas anda karjäär otsustuskorras Merko Ehitusele või kaaluda uut pakkumist

The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity.

Science.gov (United States)

Ogino, Minako; Ito, Naoto; Sugiyama, Makoto; Ogino, Tomoaki

2016-05-21

The large (L) protein of rabies virus (RABV) plays multiple enzymatic roles in viral RNA synthesis and processing. However, none of its putative enzymatic activities have been directly demonstrated in vitro. In this study, we expressed and purified a recombinant form of the RABV L protein and verified its guanosine 5'-triphosphatase and GDP polyribonucleotidyltransferase (PRNTase) activities, which are essential for viral mRNA cap formation by the unconventional mechanism. The RABV L protein capped 5'-triphosphorylated but not 5'-diphosphorylated RABV mRNA-start sequences, 5'-AACA(C/U), with GDP to generate the 5'-terminal cap structure G(5')ppp(5')A. The 5'-AAC sequence in the substrate RNAs was found to be strictly essential for RNA capping with the RABV L protein. Furthermore, site-directed mutagenesis showed that some conserved amino acid residues (G1112, T1170, W1201, H1241, R1242, F1285, and Q1286) in the PRNTase motifs A to E of the RABV L protein are required for cap formation. These findings suggest that the putative PRNTase domain in the RABV L protein catalyzes the rhabdovirus-specific capping reaction involving covalent catalysis of the pRNA transfer to GDP, thus offering this domain as a target for developing anti-viral agents.

Roll force prediction of high strength steel using foil rolling theory in cold skin pass rolling

International Nuclear Information System (INIS)

Song, Gil Ho; Jung, Jae Chook

2013-01-01

Skin pass rolling is a very important process for applying a certain elongation to a strip in the cold rolling and annealing processes, which play an important role in preventing the stretching of the yield point when the material is processed. The exact prediction of the rolling force is essential for obtaining a given elongation with the steel grade and strip size. Unlike hot rolling and cold rolling, skin pass rolling is used to apply an elongation of within 2% to the strip. Under a small reduction, it is difficult to predict the rolling force because the elastic deformation behavior of the rolls is complicated and a model for predicting the rolling force has not yet been established. Nevertheless, the exact prediction of the rolling force in skin pass rolling has gained increasing importance in recent times with the rapid development of high strength steels for use in automobiles. In this study, the possibility of predicting the rolling force in skin pass rolling for producing various steel grades was examined using foil rolling theory, which is known to have similar elastic deformation behavior of rolls in the roll bite. It was found that a noncircular arc model is more accurate than a circular model in predicting the roll force of high strength steel below TS 980 MPa in skin pass rolling

Suurõnnetuse ohuga ettevõtte õiguslikud nõuded kahjuriski vältimiseks : bakalaureusetöö / Toomas Rebane ; Tartu Ülikool, õigusteaduskond ; juhendaja: Margus Kingisepp

Index Scriptorium Estoniae

Rebane, Toomas

2010-01-01

Suurõnnetuse ohuga ettevõtte mõistest ja olemusest, riskivastutuse kohaldamise eeldustest, riskivastutust välistavatest asjaoludest, riskivastutuse ulatusest, vastutuskindlustusest kui riskivastutust toetavast instrumendist. Ettevõtte õiguslikest kohustustest kahjuriski vältimiseks

Cladding of aluminum on AISI 304L stainless steel by cold roll bonding: Mechanism, microstructure, and mechanical properties

Energy Technology Data Exchange (ETDEWEB)

Akramifard, H.R., E-mail: akrami.1367@ut.ac.ir [School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran (Iran, Islamic Republic of); Mirzadeh, H., E-mail: hmirzadeh@ut.ac.ir [School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran (Iran, Islamic Republic of); Advanced Metalforming and Thermomechanical Processing Laboratory, School of Metallurgy and Materials Engineering, University of Tehran, Tehran (Iran, Islamic Republic of); Parsa, M.H., E-mail: mhparsa@ut.ac.ir [School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran (Iran, Islamic Republic of); Center of Excellence for High Performance Materials, School of Metallurgy and Materials Engineering, University of Tehran, Tehran (Iran, Islamic Republic of); Advanced Metalforming and Thermomechanical Processing Laboratory, School of Metallurgy and Materials Engineering, University of Tehran, Tehran (Iran, Islamic Republic of)

2014-09-08

The AA1050 aluminum alloy and AISI 304L stainless steel sheets were stacked together to fabricate Al/304L/Al clad sheet composites by the cold roll bonding process, which was performed at temperatures of ∼100 and 23 °C to produce austenitic and austenitic–martensitic microstructures in the AISI 304L counterpart, respectively. The peel test results showed that the threshold reduction required to make a suitable bond at room temperature is below 10%, which is significantly lower than the required reduction for cold roll bonding of Al sheets. The tearing of the Al sheet during the peel test signified that the bond strength of the roll bonded sheets by only 38% reduction has reached the strength of Al, which is a key advantage of the developed sheets. The extrusion of Al through the surface cracks and settling inside the 304L surface valleys due to strong affinity between Al and Fe was found to be the bonding mechanism. Subsequently, the interface and tensile behaviors of three-layered clad sheets after soaking at 200–600 °C for 1 h were investigated to characterize the effect of annealing treatment on the formation and thickening of intermetallic compound layer and the resultant mechanical properties. Field emission scanning electron microscopy, X-ray diffraction, and optical microscopy techniques revealed that an intermediate layer composed mainly of Al{sub 13}Fe{sub 4}, FeC and Al{sub 8}SiC{sub 7} forms during annealing at 500–600 °C. A significant drop in tensile stress–strain curves after the maximum point (UTS) was correlated to the interface debonding. It was found that the formation of intermediate layer by post heat treatment deteriorates the bond quality and encourages the debonding process. Moreover, the existence of strain-induced martensite in clad sheets was found to play a key role in the enhancement of tensile strength.

An "off-on" electrochemiluminescent biosensor based on DNAzyme-assisted target recycling and rolling circle amplifications for ultrasensitive detection of microRNA.

Science.gov (United States)

Zhang, Pu; Wu, Xiaoyan; Yuan, Ruo; Chai, Yaqin

2015-03-17

In this study, an off-on switching of a dual amplified electrochemiluminescence (ECL) biosensor based on Pb(2+)-induced DNAzyme-assisted target recycling and rolling circle amplification (RCA) was constructed for microRNA (miRNA) detection. First, the primer probe with assistant probe and miRNA formed Y junction which was cleaved with the addition of Pb(2+) to release miRNA. Subsequently, the released miRNA could initiate the next recycling process, leading to the generation of numerous intermediate DNA sequences (S2). Afterward, bare glassy carbon electrode (GCE) was immersed into HAuCl4 solution to electrodeposit a Au nanoparticle layer (depAu), followed by the assembly of a hairpin probe (HP). Then, dopamine (DA)-modified DNA sequence (S1) was employed to hybridize with HP, which switching off the sensing system. This is the first work that employs DA to quench luminol ECL signal, possessing the biosensor ultralow background signal. Afterward, S2 produced by the target recycling process was loaded onto the prepared electrode to displace S1 and served as an initiator for RCA. With rational design, numerous repeated DNA sequences coupling with hemin to form hemin/G-quadruplex were generated, which could exhibit strongly catalytic toward H2O2, thus amplified the ECL signal and switched the ON state of the sensing system. The liner range for miRNA detection was from 1.0 fM to 100 pM with a low detection limit down to 0.3 fM. Moreover, with the high sensitivity and specificity induced by the dual signal amplification, the proposed miRNA biosensor holds great potential for analysis of other interesting tumor markers.

The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity

Directory of Open Access Journals (Sweden)

Minako Ogino

2016-05-01

Full Text Available The large (L protein of rabies virus (RABV plays multiple enzymatic roles in viral RNA synthesis and processing. However, none of its putative enzymatic activities have been directly demonstrated in vitro. In this study, we expressed and purified a recombinant form of the RABV L protein and verified its guanosine 5′-triphosphatase and GDP polyribonucleotidyltransferase (PRNTase activities, which are essential for viral mRNA cap formation by the unconventional mechanism. The RABV L protein capped 5′-triphosphorylated but not 5′-diphosphorylated RABV mRNA-start sequences, 5′-AACA(C/U, with GDP to generate the 5′-terminal cap structure G(5′ppp(5′A. The 5′-AAC sequence in the substrate RNAs was found to be strictly essential for RNA capping with the RABV L protein. Furthermore, site-directed mutagenesis showed that some conserved amino acid residues (G1112, T1170, W1201, H1241, R1242, F1285, and Q1286 in the PRNTase motifs A to E of the RABV L protein are required for cap formation. These findings suggest that the putative PRNTase domain in the RABV L protein catalyzes the rhabdovirus-specific capping reaction involving covalent catalysis of the pRNA transfer to GDP, thus offering this domain as a target for developing anti-viral agents.

The L-L oligomerization domain resides at the very N-terminus of the sendai virus L RNA polymerase protein

International Nuclear Information System (INIS)

Cevik, Bayram; Smallwood, Sherin; Moyer, Sue A.

2003-01-01

The Sendai virus RNA-dependent RNA polymerase is composed of the L and P proteins. We previously showed that the L protein gives intragenic complementation and forms an oligomer where the L-L interaction site mapped to the N-terminal half of the protein (S. Smallwood et al., 2002, Virology, 00, 000-000). We now show that L oligomerization does not depend on P protein and progressively smaller N-terminal fragments of L from amino acids (aa) 1-1146 through aa 1-174 all bind wild-type L. C-terminal truncations up to aa 424, which bind L, can complement the transcription defect in an L mutant altered at aa 379, although these L truncation mutants do not bind P. The fragment of L comprising aa 1-895, furthermore, acts as a dominant-negative mutant to inhibit transcription of wild-type L. N-terminal deletions of aa 1-189 and aa 1-734 have lost the ability to form the L-L complex as well as the L-P complex, although they still bind C protein. These data are consistent with the L-L interaction site residing in aa 1-174. Site-directed mutations in the N-terminal 347 aa, of L which abolish P binding, do not affect L-L complex formation, so while the L and P binding sites on L are overlapping they are mediated by different amino acids. The N-terminal portions of L with aa 1-424, aa 1-381, and to a lesser extent aa 1-174, can complement the transcription defect in an L mutant altered at aa 77-81, showing their L-L interaction is functional

Crystal Structure of the 23S rRNA Fragment Specific to r-Protein L1 and Designed Model of the Ribosomal L1 Stalk from Haloarcula marismortui

Directory of Open Access Journals (Sweden)

Azat Gabdulkhakov

2017-02-01

Full Text Available The crystal structure of the 92-nucleotide L1-specific fragment of 23S rRNA from Haloarcula marismortui (Hma has been determined at 3.3 Å resolution. Similar to the corresponding bacterial rRNA fragments, this structure contains joined helix 76-77 topped by an approximately globular structure formed by the residual part of the L1 stalk rRNA. The position of HmaL1 relative to the rRNA was found by its docking to the rRNA fragment using the L1-rRNA complex from Thermus thermophilus as a guide model. In spite of the anomalous negative charge of the halophilic archaeal protein, the conformation of the HmaL1-rRNA interface appeared to be very close to that observed in all known L1-rRNA complexes. The designed structure of the L1 stalk was incorporated into the H. marismortui 50S ribosomal subunit. Comparison of relative positions of L1 stalks in 50S subunits from H. marismortui and T. thermophilus made it possible to reveal the site of inflection of rRNA during the ribosome function.

Frictional resistance of adiabatic two-phase flow in narrow rectangular duct under rolling conditions

International Nuclear Information System (INIS)

Xing, Dianchuan; Yan, Changqi; Sun, Licheng; Jin, Guangyuan; Tan, Sichao

2013-01-01

Highlights: ► Two-phase flow frictional resistance in narrow duct in rolling is studied. ► Frictional resistance behaviors in rolling are divided into three regions. ► Transient frictional pressure drop fluctuates synchronously with rolling motion. ► Conventional correlations are evaluated against experimental data in rolling motion. ► New correlation for transient frictional resistance in rolling motion is developed. - Abstract: Frictional resistance of air-water two-phase flow in a narrow rectangular duct subjected to rolling motion was investigated experimentally. Time-averaged and transient frictional pressure drop under rolling condition were compared with conventional correlation in laminar flow region (Re l l ⩽ 1400) and turbulent flow region (Re l > 1400) respectively. The result shows that, despite no influence on time-averaged frictional resistance, rolling motion does induce periodical fluctuation of the pressure drop in laminar and transition flow regions. Transient frictional pressure drop fluctuates synchronously with the rolling motion both in laminar and in transition flow region, while it is nearly invariable in turbulent flow region. The fluctuation amplitude of the Relative frictional pressure gradient decreases with the increasing of the superficial velocities. Lee and Lee (2002) correlation and Chisholm (1967) correlation could satisfactorily predict time-averaged frictional pressure drop under rolling conditions, whereas poorly predict the transient frictional pressure drop when it fluctuates periodically. A new correlation with better accuracy for predicting the transient frictional pressure drop in rolling motion is achieved by modifying the Chisholm (1967) correlation on the basis of analyzing the present experimental results with a great number of data points

Rolling Process Modeling Report: Finite-Element Prediction of Roll Separating Force and Rolling Defects

Energy Technology Data Exchange (ETDEWEB)

Soulami, Ayoub [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lavender, Curt A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Paxton, Dean M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Burkes, Douglas [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

2014-04-23

Pacific Northwest National Laboratory (PNNL) has been investigating manufacturing processes for the uranium-10% molybdenum (U-10Mo) alloy plate-type fuel for the U.S. high-performance research reactors. This work supports the Convert Program of the U.S. Department of Energy’s National Nuclear Security Administration (DOE/NNSA) Global Threat Reduction Initiative. This report documents modeling results of PNNL’s efforts to perform finite-element simulations to predict roll separating forces and rolling defects. Simulations were performed using a finite-element model developed using the commercial code LS-Dyna. Simulations of the hot rolling of U-10Mo coupons encapsulated in low-carbon steel have been conducted following two different schedules. Model predictions of the roll-separation force and roll-pack thicknesses at different stages of the rolling process were compared with experimental measurements. This report discusses various attributes of the rolled coupons revealed by the model (e.g., dog-boning and thickness non-uniformity).

Terras: NATO õppuste roll Euroopas kasvab

Index Scriptorium Estoniae

2013-01-01

Kaitseväe juhataja kindralmajor Riho Terrase sõnul suureneb lähiaastatel NATO õppuste roll Euroopas, sest Afganistanis sõdides saadud kogemust on vaja säilitada ning kasutada seda kollektiivkaitse tugevdamiseks

Clusters of basic amino acids contribute to RNA binding and nucleolar localization of ribosomal protein L22.

Directory of Open Access Journals (Sweden)

Jennifer L Houmani

Full Text Available The ribosomal protein L22 is a component of the 60S eukaryotic ribosomal subunit. As an RNA-binding protein, it has been shown to interact with both cellular and viral RNAs including 28S rRNA and the Epstein-Barr virus encoded RNA, EBER-1. L22 is localized to the cell nucleus where it accumulates in nucleoli. Although previous studies demonstrated that a specific amino acid sequence is required for nucleolar localization, the RNA-binding domain has not been identified. Here, we investigated the hypothesis that the nucleolar accumulation of L22 is linked to its ability to bind RNA. To address this hypothesis, mutated L22 proteins were generated to assess the contribution of specific amino acids to RNA binding and protein localization. Using RNA-protein binding assays, we demonstrate that basic amino acids 80-93 are required for high affinity binding of 28S rRNA and EBER-1 by L22. Fluorescence localization studies using GFP-tagged mutated L22 proteins further reveal that basic amino acids 80-93 are critical for nucleolar accumulation and for incorporation into ribosomes. Our data support the growing consensus that the nucleolar accumulation of ribosomal proteins may not be mediated by a defined localization signal, but rather by specific interaction with established nucleolar components such as rRNA.

NUMERICAL EVALUATION OF TEMPERATURE DISTRIBUTION IN THE ROLLING MILL ROLLS

Directory of Open Access Journals (Sweden)

José Claudino de Lira Júnior

2013-06-01

Full Text Available In hot rolling processes occur changes in the profile of the rolling mill rolls (expansion and contraction and constant wear due to mechanical stress and continuous thermal cycles of heating/cooling caused by contact rolled material- working roll and the cooling system by water jets in their surface, decreasing their lifetime. This paper presents a computational model to simulate the thermal performance of rolling mill rolls. The model was developed using the finite volume method for a transient two-dimensional system and allows calculating the temperature distribution of the rolling mill rolls under various conditions of service. Here it is investigated the influence of flow rate and temperature of the cooling water on the temperature distribution. The results show that the water temperature has greater influence than the water flow to control the surface temperature of the cylinders.

Investigation of bending fatigue-life of aluminum sheets based on rolling direction

Directory of Open Access Journals (Sweden)

Raif Sakin

2018-03-01

Full Text Available High-cycle fatigue (HCF and low-cycle fatigue (LCF fatigue lives of rolled AA1100 and AA1050 aluminum sheets along different directions were evaluated at room temperature. Four types of samples denoted as longitudinal (L and transverse (T to the rolling direction were compared because the samples along the two typical directions show an obvious anisotropy. A cantilever plane-bending and multi-type fatigue testing machine was specially designed for this purpose. Deflection-controlled fatigue tests were conducted under fully reversed loading. The longest fatigue lives in the LCF region were obtained for AA1050 (L while AA1100 (L samples had the longest fatigue lives in the HCF region. Keywords: AA1100, AA1050, Aluminum sheet, Bending fatigue life, Rolling direction

Detailed analysis of RNA-protein interactions within the bacterial ribosomal protein L5/5S rRNA complex.

OpenAIRE

Perederina, Anna; Nevskaya, Natalia; Nikonov, Oleg; Nikulin, Alexei; Dumas, Philippe; Yao, Min; Tanaka, Isao; Garber, Maria; Gongadze, George; Nikonov, Stanislav

2002-01-01

The crystal structure of ribosomal protein L5 from Thermus thermophilus complexed with a 34-nt fragment comprising helix III and loop C of Escherichia coli 5S rRNA has been determined at 2.5 A resolution. The protein specifically interacts with the bulged nucleotides at the top of loop C of 5S rRNA. The rRNA and protein contact surfaces are strongly stabilized by intramolecular interactions. Charged and polar atoms forming the network of conserved intermolecular hydrogen bonds are located in ...

The effect of roll with passive segment on the planetary rolling process

Directory of Open Access Journals (Sweden)

Qing-Ling Zeng

2015-03-01

Full Text Available In three-roll planetary rolling process, there is secondary torsion phenomenon that may lead to rolling instability. This article proposed a new idea to alleviate the secondary torsion phenomenon by dividing the secondary torsion segment out of the roll as an independent and passive one. To study the performance of the roll with passive segment, the three-dimensional finite element models of planetary rolling process using actual roll or new roll with passive segment involving elastic–plastic and thermal–mechanical coupling were established by the software ABAQUS/Explicit, and a series of analysis had been done successfully. The rolling temperature and rolling force of planetary mill were in good agreement with the measured results, which indicated that the finite element method would supply important reference merit for three-dimensional thermo-mechanical simulation of the three-roll planetary rolling process. Comparing the simulation results of the two models, the results indicated that the change in the roll structure had just a little influence on the metal deformation, temperature, and rolling force, but it lessened the secondary torsion deformation effectively and improved the outside roundness of the rolled tube slightly. The research provided a new idea for the roll design of three-roll planetary mill (PSW.

The effect of roll gap geometry on microstructure in cold-rolled aluminum

DEFF Research Database (Denmark)

Mishin, Oleg; Bay, B.; Winther, G.

2004-01-01

Microstructure and texture are analyzed through the thickness of two aluminum plates cold-rolled 40% with different roll gap geometries. It is found that both texture and microstructure are strongly affected by the rolling geometry. After rolling with intermediate-size draughts a rolling-type tex......Microstructure and texture are analyzed through the thickness of two aluminum plates cold-rolled 40% with different roll gap geometries. It is found that both texture and microstructure are strongly affected by the rolling geometry. After rolling with intermediate-size draughts a rolling...... layers. In these layers, extended planar dislocation boundaries are frequently found to be inclined closely to the rolling direction. The subsurface and central layers of this plate exhibit microstructures similar to those in the plate rolled with intermediate draughts. It is suggested...

RNase L controls terminal adipocyte differentiation, lipids storage and insulin sensitivity via CHOP10 mRNA regulation

DEFF Research Database (Denmark)

Fabre, Odile Martine Julie; Salehzada, T; Lambert, K

2012-01-01

Adipose tissue structure is altered during obesity, leading to deregulation of whole-body metabolism. Its function depends on its structure, in particular adipocytes number and differentiation stage. To better understand the mechanisms regulating adipogenesis, we have investigated the role...... is associated with CHOP10 mRNA and regulates its stability. CHOP10 expression is conserved in RNase L(-/-)-MEFs, maintaining preadipocyte state while impairing their terminal differentiation. RNase L(-/-)-MEFs have decreased lipids storage capacity, insulin sensitivity and glucose uptake. Expression of ectopic...... RNase L in RNase L(-/-)-MEFs triggers CHOP10 mRNA instability, allowing increased lipids storage, insulin response and glucose uptake. Similarly, downregulation of CHOP10 mRNA with CHOP10 siRNA in RNase L(-/-)-MEFs improves their differentiation in adipocyte. In vivo, aged RNase L(-)/(-) mice present...

Paralogs hnRNP L and hnRNP LL exhibit overlapping but distinct RNA binding constraints.

Directory of Open Access Journals (Sweden)

Sarah A Smith

Full Text Available HnRNP (heterogeneous nuclear ribonucleoprotein proteins are a large family of RNA-binding proteins that regulate numerous aspects of RNA processing. Interestingly, several paralogous pairs of hnRNPs exist that exhibit similar RNA-binding specificity to one another, yet have non-redundant functional targets in vivo. In this study we systematically investigate the possibility that the paralogs hnRNP L and hnRNP LL have distinct RNA binding determinants that may underlie their lack of functional redundancy. Using a combination of RNAcompete and native gel analysis we find that while both hnRNP L and hnRNP LL preferentially bind sequences that contain repeated CA dinucleotides, these proteins differ in their requirement for the spacing of the CAs. Specifically, hnRNP LL has a more stringent requirement for a two nucleotide space between CA repeats than does hnRNP L, resulting in hnRNP L binding more promiscuously than does hnRNP LL. Importantly, this differential requirement for the spacing of CA dinucleotides explains the previously observed differences in the sensitivity of hnRNP L and LL to mutations within the CD45 gene. We suggest that overlapping but divergent RNA-binding preferences, as we show here for hnRNP L and hnRNP LL, may be commonplace among other hnRNP paralogs.

The RNA-binding protein, ZFP36L2, influences ovulation and oocyte maturation.

Directory of Open Access Journals (Sweden)

Christopher B Ball

Full Text Available ZFP36L2 protein destabilizes AU-rich element-containing transcripts and has been implicated in female fertility. In the C57BL/6NTac mouse, a mutation in Zfp36l2 that results in the decreased expression of a form of ZFP36L2 in which the 29 N-terminal amino acid residues have been deleted, ΔN-ZFP36L2, leads to fertilized eggs that arrest at the two-cell stage. Interestingly, homozygous ΔN-Zfp36l2 females in the C57BL/6NTac strain release 40% fewer eggs than the WT littermates (Ramos et al., 2004, suggesting an additional defect in ovulation and/or oocyte maturation. Curiously, the same ΔN-Zfp36l2 mutation into the SV129 strain resulted in anovulation, prompting us to investigate a potential problem in ovulation and oocyte maturation. Remarkably, only 20% of ΔN-Zfp36l2 oocytes in the 129S6/SvEvTac strain matured ex vivo, suggesting a defect on the oocyte meiotic maturation process. Treatment of ΔN-Zfp36l2 oocytes with a PKA inhibitor partially rescued the meiotic arrested oocytes. Furthermore, cAMP levels were increased in ΔN-Zfp36l2 oocytes, linking the cAMP/PKA pathway and ΔN-Zfp36l2 with meiotic arrest. Since ovulation and oocyte maturation are both triggered by LHR signaling, the downstream pathway was investigated. Adenylyl cyclase activity was increased in ΔN-Zfp36l2 ovaries only upon LH stimulation. Moreover, we discovered that ZFP36L2 interacts with the 3'UTR of LHR mRNA and that decreased expression levels of Zfp36l2 correlates with higher levels of LHR mRNA in synchronized ovaries. Furthermore, overexpression of ZFP36L2 decreases the endogenous expression of LHR mRNA in a cell line. Therefore, we propose that lack of the physiological down regulation of LHR mRNA levels by ZFP36L2 in the ovaries is associated with anovulation and oocyte meiotic arrest.

RELATIONSHIP BETWEEN ROLLING AND SLIP RESISTANCE IN ROLLING BEARINGS

Directory of Open Access Journals (Sweden)

L. M. Bondarenko

2016-06-01

Full Text Available Purpose. About one of the causes of slip rolling is known from the second half of the 19th century, it was believed that the slip resistance appears at the place of contact due to different speeds on the arc of contact. Only in the mid-20th century it was proved that this resistance is negligible in rolling resistance. However (for some unknown reason it is ignored the fact that in practice in rolling bearings may rotate both the inner ring with a stationary outer one, and vice versa almost in equal relations. It is not taken into account the fact that the ball or roller in the rolling bearings runs the different distance along the roller path of the outer and inner bearing cages in one revolution. This fact is not taken into account in determining the calculated values for the friction coefficient of a rolling bearing reduced to the shaft. Therefore, the aim of this work is to determine the influence of path length on the track riding the outer and inner race of the bearing on the determination of the calculated value of the coefficient of friction of rolling bearings is given to the shaft. Methodology. The solution technique is based on the theory of plane motion of a rigid body, the theory of Hertzian contact deformation and the analytical dependencies for determination of coefficient of rolling friction. Findings. The obtained dependences on determination of rolling resistance of the balls or rollers along the bearing tracks of inner and outer bearing cages as well as path difference metering of the rolling on them allows to analytically obtain the rolling resistance and slipping for any size of bearings and different devices of bearing units. It is also possible at the design stage of rolling nodes to handle not only the design but also the content of the node. Originality. Using the analytical dependences for determination of the rolling resistance of bodies at point and line contacts, and also account for the difference in the path of the

Roll-to-Roll production of carbon nanotubes based supercapacitors

Science.gov (United States)

Zhu, Jingyi; Childress, Anthony; Karakaya, Mehmet; Roberts, Mark; Arcilla-Velez, Margarita; Podila, Ramakrishna; Rao, Apparao

2014-03-01

Carbon nanomaterials provide an excellent platform for electrochemical double layer capacitors (EDLCs). However, current industrial methods for producing carbon nanotubes are expensive and thereby increase the costs of energy storage to more than 10 Wh/kg. In this regard, we developed a facile roll-to-roll production technology for scalable manufacturing of multi-walled carbon nanotubes (MWNTs) with variable density on run-of-the-mill kitchen Al foils. Our method produces MWNTs with diameter (heights) between 50-100 nm (10-100 μm), and a specific capacitance as high as ~ 100 F/g in non-aqueous electrolytes. In this talk, the fundamental challenges involved in EDLC-suitable MWNT growth, roll-to-roll production, and device manufacturing will be discussed along with electrochemical characteristics of roll-to-roll MWNTs. Research supported by NSF CMMI Grant1246800.

Haavatutelaeva Steuben hukkumine Läänemerel / Vaino Kallas

Index Scriptorium Estoniae

Kallas, Vaino, 1927-

2008-01-01

Sakslaste reisilaevast Wilhelm Gustloffist, millel oli suur roll Teises maailmasõjas. Samal teemal : Aino Siebert "Sakslaste trauma - Wilhelm Gustloffi hukkumine" (Pärnu Postimees 17.07.08) ja Küllo Arjakas "Täienduseks Saksa reisilaeva Wilhelm Gustloffi hukkumise loole" (Pärnu Postimees 29.08.08)

A Novel Association between Two Trypanosome-Specific Factors and the Conserved L5-5S rRNA Complex

Science.gov (United States)

Ciganda, Martin; Prohaska, Kimberly; Hellman, Kristina; Williams, Noreen

2012-01-01

P34 and P37 are two previously identified RNA binding proteins in the flagellate protozoan Trypanosoma brucei. RNA interference studies have determined that the proteins are involved in and essential for ribosome biogenesis. The proteins interact with the 5S rRNA with nearly identical binding characteristics. We have shown that this interaction is achieved mainly through the LoopA region of the RNA, but P34 and P37 also protect the L5 binding site located on LoopC. We now provide evidence to show that these factors form a novel pre-ribosomal particle through interactions with both 5S rRNA and the L5 ribosomal protein. Further in silico and in vitro analysis of T. brucei L5 indicates a lower affinity for 5S rRNA than expected, based on other eukaryotic L5 proteins. We hypothesize that P34 and P37 complement L5 and bridge the interaction with 5S rRNA, stabilizing it and aiding in the early steps of ribosome biogenesis. PMID:22859981

l-Proline and RNA Duplex m-Value Temperature Dependence.

Science.gov (United States)

Schwinefus, Jeffrey J; Baka, Nadia L; Modi, Kalpit; Billmeyer, Kaylyn N; Lu, Shutian; Haase, Lucas R; Menssen, Ryan J

2017-08-03

The temperature dependence of l-proline interactions with the RNA dodecamer duplex surface exposed after unfolding was quantified using thermal and isothermal titration denaturation monitored by uv-absorbance. The m-value quantifying proline interactions with the RNA duplex surface area exposed after unfolding was measured using RNA duplexes with GC content ranging between 17 and 83%. The m-values from thermal denaturation decreased with increasing GC content signifying increasingly favorable proline interactions with the exposed RNA surface area. However, m-values from isothermal titration denaturation at 25.0 °C were independent of GC content and less negative than those from thermal denaturation. The m-value from isothermal titration denaturation for a 50% GC RNA duplex decreased (became more negative) as the temperature increased and was in nearly exact agreement with the m-value from thermal denaturation. Since RNA duplex transition temperatures increased with GC content, the more favorable proline interactions with the high GC content duplex surface area observed from thermal denaturation resulted from the temperature dependence of proline interactions rather than the RNA surface chemical composition. The enthalpy contribution to the m-value was positive and small (indicating a slight increase in duplex unfolding enthalpy with proline) while the entropic contribution to the m-value was positive and increased with temperature. Our results will facilitate proline's use as a probe of solvent accessible surface area changes during biochemical reactions at different reaction temperatures.

HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses

International Nuclear Information System (INIS)

Rollman, Erik; Arnheim, Lisen; Collier, Brian; Oeberg, Daniel; Hall, Haakan; Klingstroem, Jonas; Dillner, Joakim; Pastrana, Diana V.; Buck, Chris B.; Hinkula, Jorma; Wahren, Britta; Schwartz, Stefan

2004-01-01

Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57Bl/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus

Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre

DEFF Research Database (Denmark)

Rosendahl, G; Douthwaite, S

1993-01-01

RNA, and to investigate how this interaction is influenced by other ribosomal components. Complexes were characterized in both naked 23 S rRNA and ribosomes from an E. coli L11-minus strain, before and after reconstitution with L11. The protein protects 17 riboses between positions 1058 and 1085 in the naked 23 S r......The Escherichia coli ribosomal protein (r-protein) L11 and its binding site on 23 S ribosomal RNA (rRNA) are associated with ribosomal hydrolysis of guanosine 5'-triphosphate (GTP). We have used hydroxyl radical footprinting to map the contacts between L11 and the backbone riboses in 23 S r......)4 and other proteins within the ribosome. The antibiotics thiostrepton and micrococcin inhibit the catalytic functions of this region by slotting in between the accessible loops and interacting with nucleotides there....

Influence of ozone on RNA and protein content of Lemna minor L

Energy Technology Data Exchange (ETDEWEB)

Craker, L E

1972-01-01

The amount of RNA and protein in Lemna minor L. plants decreased after exposure to ozone, as compared with control plants receiving no ozone treatment. Differences in RNA and protein content between control and ozone-treated Lemna plants were detectable immediately following ozone treatments and remained throughout the 24 h sampling time.

LncRNA TUG1 sponges microRNA-9 to promote neurons apoptosis by up-regulated Bcl2l11 under ischemia.

Science.gov (United States)

Chen, Shengcai; Wang, Mengdie; Yang, Hang; Mao, Ling; He, Quanwei; Jin, Huijuan; Ye, Zi-Ming; Luo, Xue-Ying; Xia, Yuan-Peng; Hu, Bo

2017-03-25

Emerging studies have illustrated that LncRNAs TUG1 play critical roles in multiple biologic processes. However, the LncRNA TUG1 expression and function in ischemic stroke have not been reported yet. In this study, we found that LncRNA TUG1 expression was significantly up-regulated in brain ischemic penumbra from rat middle carotid artery occlusion (MCAO) model, while similar results were also observed in cultured neurons under oxygen-glucose deprivation (OGD) insult. Knockdown of TUG1 decreased the ratio of apoptotic cells and promoted cells survival in vitro, which may be regulated by the elevated miRNA-9 expression and decreased Bcl2l11 protein. Furthermore, TUG1 could directly interact with miR-9 and down-regulating miR-9 could efficiently reverse the function of TUG1 on the Bcl2l11 expression. In summary, our result sheds light on the role of LncRNA TUG1 as a miRNA sponge for ischemic stroke, possibly providing a new therapeutic target in stroke. Copyright © 2017 Elsevier Inc. All rights reserved.

Double-blind randomized controlled trial of rolls fortified with microencapsulated iron.

Science.gov (United States)

Barbosa, Teresa Negreira Navarro; Taddei, José Augusto de Aguiar Carrazedo; Palma, Domingos; Ancona-Lopez, Fábio; Braga, Josefina Aparecida Pellegrini

2012-01-01

To evaluate the impact of the fortification of rolls with microencapsulated iron sulfate with sodium alginate on the hemoglobin levels in preschoolers as compared to controls. Double-blind randomized controlled trial comprised of children aged 2 to 6 years with initial hemoglobin exceeding 9 g/dL from four not-for-profit daycares randomly selected in the city of São Paulo - Brazil. Children of 2 daycares (n = 88) received rolls with fortified wheat flour as the exposed group (EC) and children of 2 daycares (n = 85) received rolls without fortification as the control group (CG) over a 24-week period. Rolls with 4 mg iron each were offered once a day, five days a week. Hemoglobin concentrations were determined in capillary blood by HemoCue® at three moments of trial: baseline (Ml), after 12 and 24 weeks of intervention (M2, M3). Hemoglobin concentration presented significant increase up to M3 in EG (11.7-12.5-12.6 g/dL) and in CG (11.1-12.4-12.3 g/dL) with higher elevations in children initially with anemia. There was significant reduction in the occurrence of anemia from 22% to 9% in EG and from 47% to 8.2% in CG at M3. Rolls fortified with microencapsulated iron sulfate were well tolerated, increased hemoglobin levels and reduced the occurrence of anemia, but with no difference compared to the control group.

Manufacturing Demonstration Facility: Roll-to-Roll Processing

Energy Technology Data Exchange (ETDEWEB)

Datskos, Panos G [ORNL; Joshi, Pooran C [ORNL; List III, Frederick Alyious [ORNL; Duty, Chad E [ORNL; Armstrong, Beth L [ORNL; Ivanov, Ilia N [ORNL; Jacobs, Christopher B [ORNL; Graham, David E [ORNL; Moon, Ji Won [ORNL

2015-08-01

This Manufacturing Demonstration Facility (MDF)e roll-to-roll processing effort described in this report provided an excellent opportunity to investigate a number of advanced manufacturing approaches to achieve a path for low cost devices and sensors. Critical to this effort is the ability to deposit thin films at low temperatures using nanomaterials derived from nanofermentation. The overarching goal of this project was to develop roll-to-roll manufacturing processes of thin film deposition on low-cost flexible substrates for electronics and sensor applications. This project utilized ORNL s unique Pulse Thermal Processing (PTP) technologies coupled with non-vacuum low temperature deposition techniques, ORNL s clean room facility, slot dye coating, drop casting, spin coating, screen printing and several other equipment including a Dimatix ink jet printer and a large-scale Kyocera ink jet printer. The roll-to-roll processing project had three main tasks: 1) develop and demonstrate zinc-Zn based opto-electronic sensors using low cost nanoparticulate structures manufactured in a related MDF Project using nanofermentation techniques, 2) evaluate the use of silver based conductive inks developed by project partner NovaCentrix for electronic device fabrication, and 3) demonstrate a suite of low cost printed sensors developed using non-vacuum deposition techniques which involved the integration of metal and semiconductor layers to establish a diverse sensor platform technology.

Право в годы Великой Отечественной войны

Index Scriptorium Estoniae

2015-01-01

Terve ajakirja number on pühendatud erinevatele õigusküsimustele Suure Isamaasõja ajal: mobilisatsiooniõigus, meditsiiniõigus, kiriku roll, võitlus kuritegevusega, sõjavangide tööle rakendamine, kaadri ettevalmistamine töölaagrite kohtutele, vastuluure tegevus, töösuhete reguleerimine jne.

Inflationary dynamics with a smooth slow-roll to constant-roll era transition

Energy Technology Data Exchange (ETDEWEB)

Odintsov, S.D. [ICREA, Passeig Luis Companys, 23, 08010 Barcelona (Spain); Oikonomou, V.K., E-mail: odintsov@ieec.uab.es, E-mail: v.k.oikonomou1979@gmail.com [Laboratory for Theoretical Cosmology, Tomsk State University of Control Systems and Radioelectronics (TUSUR), Lenin Avenue 40, 634050 Tomsk (Russian Federation)

2017-04-01

In this paper we investigate the implications of having a varying second slow-roll index on the canonical scalar field inflationary dynamics. We shall be interested in cases that the second slow-roll can take small values and correspondingly large values, for limiting cases of the function that quantifies the variation of the second slow-roll index. As we demonstrate, this can naturally introduce a smooth transition between slow-roll and constant-roll eras. We discuss the theoretical implications of the mechanism we introduce and we use various illustrative examples in order to better understand the new features that the varying second slow-roll index introduces. In the examples we will present, the second slow-roll index has exponential dependence on the scalar field, and in one of these cases, the slow-roll era corresponds to a type of α-attractor inflation. Finally, we briefly discuss how the combination of slow-roll and constant-roll may lead to non-Gaussianities in the primordial perturbations.

The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA

Science.gov (United States)

Lai, Stella M.; Lai, Lien B.; Foster, Mark P.; Gopalan, Venkat

2014-01-01

The RNA-binding protein L7Ae, known for its role in translation (as part of ribosomes) and RNA modification (as part of sn/oRNPs), has also been identified as a subunit of archaeal RNase P, a ribonucleoprotein complex that employs an RNA catalyst for the Mg2+-dependent 5′ maturation of tRNAs. To better understand the assembly and catalysis of archaeal RNase P, we used a site-specific hydroxyl radical-mediated footprinting strategy to pinpoint the binding sites of Pyrococcus furiosus (Pfu) L7Ae on its cognate RNase P RNA (RPR). L7Ae derivatives with single-Cys substitutions at residues in the predicted RNA-binding interface (K42C/C71V, R46C/C71V, V95C/C71V) were modified with an iron complex of EDTA-2-aminoethyl 2-pyridyl disulfide. Upon addition of hydrogen peroxide and ascorbate, these L7Ae-tethered nucleases were expected to cleave the RPR at nucleotides proximal to the EDTA-Fe–modified residues. Indeed, footprinting experiments with an enzyme assembled with the Pfu RPR and five protein cofactors (POP5, RPP21, RPP29, RPP30 and L7Ae–EDTA-Fe) revealed specific RNA cleavages, localizing the binding sites of L7Ae to the RPR's catalytic and specificity domains. These results support the presence of two kink-turns, the structural motifs recognized by L7Ae, in distinct functional domains of the RPR and suggest testable mechanisms by which L7Ae contributes to RNase P catalysis. PMID:25361963

Rapid RNase L-driven arrest of protein synthesis in the dsRNA response without degradation of translation machinery.

Science.gov (United States)

Donovan, Jesse; Rath, Sneha; Kolet-Mandrikov, David; Korennykh, Alexei

2017-11-01

Mammalian cells respond to double-stranded RNA (dsRNA) by activating a translation-inhibiting endoribonuclease, RNase L. Consensus in the field indicates that RNase L arrests protein synthesis by degrading ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs). However, here we provide evidence for a different and far more efficient mechanism. By sequencing abundant RNA fragments generated by RNase L in human cells, we identify site-specific cleavage of two groups of noncoding RNAs: Y-RNAs, whose function is poorly understood, and cytosolic tRNAs, which are essential for translation. Quantitative analysis of human RNA cleavage versus nascent protein synthesis in lung carcinoma cells shows that RNase L stops global translation when tRNAs, as well as rRNAs and mRNAs, are still intact. Therefore, RNase L does not have to degrade the translation machinery to stop protein synthesis. Our data point to a rapid mechanism that transforms a subtle RNA cleavage into a cell-wide translation arrest. © 2017 Donovan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

Study of wheat breakfast rolls fortified with folic acid. The effect on folate status in women during a 3-month intervention.

Science.gov (United States)

Johansson, Madelene; Witthöft, Cornelia M; Bruce, Ake; Jägerstad, Margaretha

2002-12-01

Folate has come into focus due to its protective role against child birth defects such as neural tube defects (NTD). Swedish authorities recommend all fertile women to increase their folate intake to 400 microg/day by eating folate-rich foods. Because not all women follow these recommendations, there is a discussion today about whether Sweden should introduce folic acid fortification in wheat flour and sifted rye flour. This decision needs knowledge about the bioavailability of folic acid from fortified foods. To investigate effects of two folic acid fortification levels on folate status in healthy female volunteers and to study the folic acid stability during the baking procedure and storage of the fortified breakfast rolls. Twenty-nine healthy women were recruited. Folic acid-fortified wheat breakfast rolls were baked with the purpose to contain 200 microg folic acid/roll (roll L) and 400 microg folic acid/roll (roll H). Fourteen women were given one roll/day of roll L (group L) and 15 one roll/day of roll H (group H) during 12 weeks of intervention. Fasting venous blood samples were collected on days 0, 30, 60 and 90. Serum homocysteine concentrations were determined using an immunoassay. Serum and erythrocyte folate concentrations were analysed using a protein-binding assay with fluorescent quantification. The folic acid concentration in the breakfast rolls was analysed by HPLC on days 0, 30, 60 and 90. Total folate concentration was measured with microbiological assay on day 45. Group L Group L had initially an average erythrocyte folate concentration of 577 +/- 93 nmol/L. After 90 days of intervention, an increase of 20 % (p level of 18.7 +/- 4.8 nmol/L at day 0. Thereafter, all women of group H had serum concentrations at or above the upper limit of quantification (23 nmol/L). At day 0, mean serum homocysteine concentrations were 8.4 +/- 1.7 micromol/L, which decreased by 16 % (p level of fortification seems to be sufficient to improve the folate status

COMPARISON OF 16S rRNA-PCR-RFLP, LipL32-PCR AND OmpL1-PCR METHODS IN THE DIAGNOSIS OF LEPTOSPIROSIS

Directory of Open Access Journals (Sweden)

Tülin GÜVEN GÖKMEN

Full Text Available SUMMARY Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of infected wild and domestic animals such as rodents and dogs. The clinical course of leptospirosis is variable and may be difficult to distinguish from many other infectious diseases. The dark-field microscopy (DFM, serology and nucleic acid amplification techniques are used to diagnose leptospirosis, however, a distinctive standard reference method is still lacking. Therefore, in this study, we aimed to determine the presence of Leptospira spp., to differentiate the pathogenic L. interrogans and the non-pathogenic L. biflexa, and also to determine the sensitivity and specificity values of molecular methods as an alternative to conventional ones. A total of 133 serum samples, from 47 humans and 86 cattle were evaluated by two conventional tests: the Microagglutination Test (MAT and the DFM, as well as three molecular methods, the 16S rRNA-PCR followed by Restriction Fragment Lenght Polymorphism (RFLP of the amplification products 16S rRNA-PCR-RFLP, LipL32-PCR and OmpL1-PCR. In this study, for L. interrogans, the specificity and sensitivity rates of the 16S rRNA-PCR and the LipL32-PCR were considered similar (100% versus 98.25% and 100% versus 98.68%, respectively. The OmpL1-PCR was able to classify L. interrogans into two intergroups, but this PCR was less sensitive (87.01% than the other two PCR methods. The 16S rRNA-PCR-RFLP could detect L. biflexa DNA, but LipL32-PCR and OmpL1-PCR could not. The 16S rRNA-PCR-RFLP provided an early and accurate diagnosis and was able to distinguish pathogenic and non-pathogenic Leptospira species, hence it may be used as an alternative method to the conventional gold standard techniques for the rapid disgnosis of leptospirosis.

Computer-aided roll pass design in rolling of airfoil shapes

Science.gov (United States)

Akgerman, N.; Lahoti, G. D.; Altan, T.

1980-01-01

This paper describes two computer-aided design (CAD) programs developed for modeling the shape rolling process for airfoil sections. The first program, SHPROL, uses a modular upper-bound method of analysis and predicts the lateral spread, elongation, and roll torque. The second program, ROLPAS, predicts the stresses, roll separating force, the roll torque and the details of metal flow by simulating the rolling process, using the slab method of analysis. ROLPAS is an interactive program; it offers graphic display capabilities and allows the user to interact with the computer via a keyboard, CRT, and a light pen. The accuracy of the computerized models was evaluated by (a) rolling a selected airfoil shape at room temperature from 1018 steel and isothermally at high temperature from Ti-6Al-4V, and (b) comparing the experimental results with computer predictions. The comparisons indicated that the CAD systems, described here, are useful for practical engineering purposes and can be utilized in roll pass design and analysis for airfoil and similar shapes.

Bottle roll leach test for Temrezli uranium ore

International Nuclear Information System (INIS)

Çetin, K.; Bayrak, M.; Turan, A. İsbir; Üçgül, E.

2014-01-01

The bottle roll leach test is one of the dynamic leaching procedure which can meet in-situ mining needs for determining suitable working conditions and helps to simulate one of the important parameter; injection well design. In this test, the most important parameters are pulp density, acidic or basic concentration of leach solution, time and temperature. In recent years, bottle roll test is used not only for uranium but also gold, silver, copper and nickel metals where in situ leach (ISL) mining is going to be applied. For this purpose for gold and silver metal cyanide bottle roll tests and for uranium metal; acidic and basic bottle roll tests could be applied. The new leach test procedure which is held in General Directorate of Mineral Research and Exploration (MTA) of Turkey is mostly suitable for determining metal extraction conditions and recovery values in uranium containing ore bodies. The tests were conducted with samples taken from Temrezli Uranium Ore located in approximately 200 km east of Turkey’s capital, Ankara. Mining rights of Temrezli Ore is controlled 100% by Anatolia Energy Ltd. The resource estimate includes an indicated mineral resource of 10.827 Mlbs U_3O_8 [~4160 t U] at an average grade of 1426 ppm [~1210 ppm U] and an additional inferred resource of 6.587 Mlbs of U_3O_8 [~2530 t U] at an average grade of 904 ppm [~767 ppm U]. In accordance with the demand from Anatolia Energy bottle roll leach tests have been initiated in MTA laboratories to investigate the recovery values of low-grade uranium ore under in-situ leach conditions. Bottle roll leaching tests are performed on pulverized samples with representative lixiviant solution at ambient pressure and provide an initial evaluation of ore leachability with a rough estimate of recovery value. At the end of the tests by using 2 g/L NaHCO_3 and 0.2 g/L H_2O_2 more than 90% of uranium can pass into leach solution in 12 days. (author)

Processing of fine grained AISI 304L austenitic stainless steel by cold rolling and high-temperature short-term annealing

Science.gov (United States)

Naghizadeh, Meysam; Mirzadeh, Hamed

2018-05-01

An advanced thermomechanical process based on the formation and reversion of deformation-induced martensite was used to refine the grain size and enhance the hardness of an AISI 304L austenitic stainless steel. Both low and high reversion annealing temperatures and also the repetition of the whole thermomechanical cycle were considered. While a microstructure with average austenite grain size of a few micrometers was achieved based on cold rolling and high-temperature short-term annealing, an extreme grain refinement up to submicrometer regime was obtained by cold rolling followed by low-temperature long-term annealing. However, the required annealing time was found to be much longer, which negates its appropriateness for industrial production. While a magnificent grain refinement was achieved by one pass of the high-temperature thermomechanical process, the reduction in grain size was negligible by the repetition of the whole cycle. It was found that the hardness of the thermomechanically processed material is much higher than that of the as-received material. The results of the present work were shown to be compatible with the general trend of grain size dependence of hardness for AISI 304L stainless steel based on the Hall-Petch relationship. The results were also discussed based on the X-ray evaluation of dislocation density by modified Williamson-Hall plots.

Avian reovirus L2 genome segment sequences and predicted structure/function of the encoded RNA-dependent RNA polymerase protein

Directory of Open Access Journals (Sweden)

Xu Wanhong

2008-12-01

Full Text Available Abstract Background The orthoreoviruses are infectious agents that possess a genome comprised of 10 double-stranded RNA segments encased in two concentric protein capsids. Like virtually all RNA viruses, an RNA-dependent RNA polymerase (RdRp enzyme is required for viral propagation. RdRp sequences have been determined for the prototype mammalian orthoreoviruses and for several other closely-related reoviruses, including aquareoviruses, but have not yet been reported for any avian orthoreoviruses. Results We determined the L2 genome segment nucleotide sequences, which encode the RdRp proteins, of two different avian reoviruses, strains ARV138 and ARV176 in order to define conserved and variable regions within reovirus RdRp proteins and to better delineate structure/function of this important enzyme. The ARV138 L2 genome segment was 3829 base pairs long, whereas the ARV176 L2 segment was 3830 nucleotides long. Both segments were predicted to encode λB RdRp proteins 1259 amino acids in length. Alignments of these newly-determined ARV genome segments, and their corresponding proteins, were performed with all currently available homologous mammalian reovirus (MRV and aquareovirus (AqRV genome segment and protein sequences. There was ~55% amino acid identity between ARV λB and MRV λ3 proteins, making the RdRp protein the most highly conserved of currently known orthoreovirus proteins, and there was ~28% identity between ARV λB and homologous MRV and AqRV RdRp proteins. Predictive structure/function mapping of identical and conserved residues within the known MRV λ3 atomic structure indicated most identical amino acids and conservative substitutions were located near and within predicted catalytic domains and lining RdRp channels, whereas non-identical amino acids were generally located on the molecule's surfaces. Conclusion The ARV λB and MRV λ3 proteins showed the highest ARV:MRV identity values (~55% amongst all currently known ARV and MRV

Inflation with a smooth constant-roll to constant-roll era transition

Science.gov (United States)

Odintsov, S. D.; Oikonomou, V. K.

2017-07-01

In this paper, we study canonical scalar field models, with a varying second slow-roll parameter, that allow transitions between constant-roll eras. In the models with two constant-roll eras, it is possible to avoid fine-tunings in the initial conditions of the scalar field. We mainly focus on the stability of the resulting solutions, and we also investigate if these solutions are attractors of the cosmological system. We shall calculate the resulting scalar potential and, by using a numerical approach, we examine the stability and attractor properties of the solutions. As we show, the first constant-roll era is dynamically unstable towards linear perturbations, and the cosmological system is driven by the attractor solution to the final constant-roll era. As we demonstrate, it is possible to have a nearly scale-invariant power spectrum of primordial curvature perturbations in some cases; however, this is strongly model dependent and depends on the rate of the final constant-roll era. Finally, we present, in brief, the essential features of a model that allows oscillations between constant-roll eras.

Upscaling of polymer solar cell fabrication using full roll-to-roll processing

DEFF Research Database (Denmark)

Krebs, Frederik C; Tromholt, Thomas; Jørgensen, Mikkel

2010-01-01

factors (excluding bus bars) of 50, 67 and 75% respectively. In addition modules with lengths of 6, 10, 20, 22.5 and 25 cm were explored. The devices were prepared by full roll-to-roll solution processing in a web width of 305 mm and roll lengths of up to 200 m. The devices were encapsulated...... with a barrier material in a full roll-to-roll process using standard adhesives giving the devices excellent stability during storage and operation. The total area of processed polymer solar cell was around 60 m2 per run. The solar cells were characterised using a roll-to-roll system comprising a solar simulator...... to the cost for electricity using existing technologies the levelized cost of electricity (LCOE) is expected to be significantly higher than the existing technologies due to the inferior operational lifetime. The presented devices are thus competitive for consumer electronics but ill-suited for on...

The effects of cold rolling orientation and water chemistry on stress corrosion cracking behavior of 316L stainless steel in simulated PWR water environments

Energy Technology Data Exchange (ETDEWEB)

Chen, Junjie [Institute of Materials Science, School of Materials Science and Engineering, Shanghai University, Mailbox 269, 149 Yanchang Road, Shanghai, 200072 (China); Lu, Zhanpeng, E-mail: zplu@t.shu.edu.cn [Institute of Materials Science, School of Materials Science and Engineering, Shanghai University, Mailbox 269, 149 Yanchang Road, Shanghai, 200072 (China); State Key Laboratory of Advanced Special Steels, Shanghai University, 149 Yanchang Road, Shanghai, 200072 (China); Xiao, Qian; Ru, Xiangkun; Han, Guangdong; Chen, Zhen [Institute of Materials Science, School of Materials Science and Engineering, Shanghai University, Mailbox 269, 149 Yanchang Road, Shanghai, 200072 (China); Zhou, Bangxin [Institute of Materials Science, School of Materials Science and Engineering, Shanghai University, Mailbox 269, 149 Yanchang Road, Shanghai, 200072 (China); State Key Laboratory of Advanced Special Steels, Shanghai University, 149 Yanchang Road, Shanghai, 200072 (China); Shoji, Tetsuo [New Industry Creation Hatchery Center, Tohoku University, Sendai 980-8579 (Japan)

2016-04-15

Stress corrosion cracking behaviors of one-directionally cold rolled 316L stainless steel specimens in T–L and L–T orientations were investigated in hydrogenated and deaerated PWR primary water environments at 310 °C. Transgranular cracking was observed during the in situ pre-cracking procedure and the crack growth rate was almost not affected by the specimen orientation. Locally intergranular stress corrosion cracks were found on the fracture surfaces of specimens in the hydrogenated PWR water. Extensive intergranular stress corrosion cracks were found on the fracture surfaces of specimens in deaerated PWR water. More extensive cracks were found in specimen T–L orientation with a higher crack growth rate than that in the specimen L–T orientation with a lower crack growth rate. Crack branching phenomenon found in specimen L–T orientation in deaerated PWR water was synergistically affected by the applied stress direction as well as the preferential oxidation path along the elongated grain boundaries, and the latter was dominant. - Highlights: • Transgranular fatigue crack growth rate was not affected by the cold rolling orientation. • Locally intergranular SCC was found in the hydrogenated PWR water. • Extensive intergranular SCC cracks were found in deaerated PWR water. • T–L specimen showed more extensive SCC cracks and a higher crack growth rate. • Crack branching related to the applied stress and the preferential oxidation path.

Does unpaired adenosine-66 from helix II of Escherichia coli 5S RNA bind to protein L18?

DEFF Research Database (Denmark)

Christiansen, J; Douthwaite, S R; Christensen, A

1985-01-01

Adenosine-66 is unpaired within helix II of Escherichia coli 5S RNA and lies in the binding site of ribosomal protein L18. It has been proposed as a recognition site for protein L18. We have investigated further the structural importance of this nucleotide by deleting it. The 5S RNA gene of the rrn...... plasmid derived from pKK3535. Binding studies with protein L18 revealed that the protein bound much more weakly to the mutated 5S RNA. We consider the most likely explanation of this result is that L18 interacts with adenosine-66, and we present a tentative model for an interaction between the unpaired...

Plasmonic color metasurfaces fabricated by a high speed roll-to-roll method

DEFF Research Database (Denmark)

Murthy, Swathi; Pranov, Henrik; Feidenhans'l, Nikolaj Agentoft

2017-01-01

Lab-scale plasmonic color printing using nano-structured and subsequently metallized surfaces have been demonstrated to provide vivid colors. However, upscaling these structures for large area manufacturing is extremely challenging due to the requirement of nanometer precision of metal thickness....... In this study, we have investigated a plasmonic color meta-surface design that can be easily upscaled. We have demonstrated the feasibility of fabrication of these plasmonic color surfaces by a high-speed roll-to-roll method, comprising roll-to-roll extrusion coating at 10 m min-1 creating a polymer foil having...... 100 nm deep pits of varying sub-wavelength diameter and pitch length. Subsequently this polymer foil was metallized and coated also by high-speed roll-to-roll methods. The perceived colors have high tolerance towards the thickness of the metal layer, when this thickness exceeds the depths of the pits...

Changes in the synthesis of DNA, RNA and protein during somatic embryogenesis in wheat (triticum aestivum L.)

International Nuclear Information System (INIS)

Cui Kairong; Wang Xiaozhe; Chen Xiong; Wang Yafu

1997-01-01

Embryogenic and non-embryogenic callus formed from immature embryo of wheat (Triticum aestivum L.) in N 6 B 5 MS medium I supplemented with 2,4-D 2 mg/L, KT 0.5 mg/L, LH300 mg/L, sucrose 3% were sub-cultured and transferred respectively to N 6 B 5 MS medium II (2,4-D was decreased to 0.5 mg/L and 4 mol/L proline was added). Somatic embryos obtained from embryogenic callus, and plantlet formed from non-embryogenic callus through organogenesis respectively. By incorporation of 3 H-thymidine, 3 H-uridine and 3 H-leucine into DNA, RNA and protein respectively, the rate of synthesis of DNA, RNA and protein during somatic embryogenesis were measured. A large amount of RNA and protein synthesized during the early somatic embryogenesis. The activities of RNA and protein synthesis reached the peak on the 4th and the 8th day respectively, then decreased a little, but kept a high level. The synthesis of DNA increased apparently during the early stage. No apparent change occurred when the embryogenic cell masses formed. The synthesis rate of RNA and protein in non-embryogenic callus were much less than that in embryogenic callus. Actinomycin and cycloheximide inhibited not only the synthesis of nucleic acid and protein, but also the growth of embryogenic callus and somatic embryogenesis. The earlier the inhibitors were added, the greater the influence was caused. The results indicate that the active expression of corresponding genes of wheat is the molecular base of somatic embryogenesis

Fabrication of cold-rolled bands of the alloy-ehi 702 in rolls

International Nuclear Information System (INIS)

Zhuchin, V.N.; Gindin, A.Sh.; Shaburov, V.E.; Vladimirov, S.M.; Sokolov, V.A.; Shavkun, V.V.; Perepelitsa, I.V.; Markov, V.V.; Naymov, E.P.; Evstaf'ev, P.P.

1977-01-01

The questions are discussed, connected with the manufacture of cold-rolled strip of alloy EI702 in reels from strip blanks. It has been established that in the manufacture of hot-rolled stock from EI702 slabs it is necessary to use powerful rolling equipment because of high resistance to deformation. The reel method for manufacturing EI702 alloy improves the rolled stock and increases percentage of serviceable stock, as well as the output

Stress and accidental defect detection on rolling mill rolls

International Nuclear Information System (INIS)

Auzas, J.-D.

1999-01-01

During the rolling mill process, rolls are submitted to high pressures that can lead to local decohesion or metallurgical changes. Both these cracks or softened areas must be detected as soon as they appear because of the risk of spalling, marks on the product, and mill wreck. These defects can be detected using the eddy current method, and particularly sensors specially developed for micro-defects detection. These sensors must be adapted to the environment of a roll grinding machine on which they must be installed. Users' schedule of conditions also require them to be attached to a wide range of eddy current generator and automatic computerized interpretation. Mill requirements for new high tech roll grades and quality lead to continuous development and improvement of the tools that will provide immediate 'go - no go' information. This paper is an update of these developments. (author)

Rolling and slipping down Galileo close-quote s inclined plane: Rhythms of the spheres

International Nuclear Information System (INIS)

Crawford, F.S.

1996-01-01

In ''Two New Sciences'' (TNS) Galileo presents a number of theorems and propositions for smooth solid spheres released from rest and rolling a distance d in time t down an incline of height H and length L. We collect and summarize his results in a single grand proportionality P: d 1 /d 2 =(t 2 1 /t 2 2 )(H/L) 1 /(H/L) 2 . (P) From TNS it is clear that what we call P is assumed by Galileo to hold for all inclinations including vertical free fall with H/L=1. But in TNS he describes only experiments with gentle inclinations H/L 1 while rolling down a gentle incline is deflected so as to be launched horizontally with speed v 1 into a free fall orbit discovered by Galileo to be a parabola. The measured horizontal distance X 2 traveled in this parabolic orbit (for a given vertical distance fallen to the floor) was smaller than he expected, by a factor 0.84. But that is exactly what we (moderns) expect, since we know that Galileo did not appreciate the difference between rolling without slipping, and slipping on a frictionless surface

Effects of False Tilt Cues on the Training of Manual Roll Control Skills

Science.gov (United States)

Zaal, Peter M. T.; Popovici, Alexandru; Zavala, Melinda A.

2015-01-01

This paper describes a transfer-of-training study performed in the NASA Ames Vertica lMotion Simulator. The purpose of the study was to investigate the effect of false tilt cues on training and transfer of training of manual roll control skills. Of specific interest were the skills needed to control unstable roll dynamics of a mid-size transport aircraft close to the stall point. Nineteen general aviation pilots trained on a roll control task with one of three motion conditions: no motion, roll motion only, or reduced coordinated roll motion. All pilots transferred to full coordinated roll motion in the transfer session. A novel multimodal pilot model identification technique was successfully applied to characterize how pilots' use of visual and motion cues changed over the course of training and after transfer. Pilots who trained with uncoordinated roll motion had significantly higher performance during training and after transfer, even though they experienced the false tilt cues. Furthermore, pilot control behavior significantly changed during the two sessions, as indicated by increasing visual and motion gains, and decreasing lead time constants. Pilots training without motion showed higher learning rates after transfer to the full coordinated roll motion case.

Spray rolling aluminum alloy strip

Energy Technology Data Exchange (ETDEWEB)

McHugh, Kevin M.; Delplanque, J.-P.; Johnson, S.B.; Lavernia, E.J.; Zhou, Y.; Lin, Y

2004-10-10

Spray rolling combines spray forming with twin-roll casting to process metal flat products. It consists of atomizing molten metal with a high velocity inert gas, cooling the resultant droplets in flight and directing the spray between mill rolls. In-flight convection heat transfer from atomized droplets teams with conductive cooling at the rolls to rapidly remove the alloy's latent heat. Hot deformation of the semi-solid material in the rolls results in fully consolidated, rapidly solidified product. While similar in some ways to twin-roll casting, spray rolling has the advantage of being able to process alloys with broad freezing ranges at high production rates. This paper describes the process and summarizes microstructure and tensile properties of spray-rolled 2124 and 7050 aluminum alloy strips. A Lagrangian/Eulerian poly-dispersed spray flight and deposition model is described that provides some insight into the development of the spray rolling process. This spray model follows droplets during flight toward the rolls, through impact and spreading, and includes oxide film formation and breakup when relevant.

Replication of nanopits and nanopillars by roll-to-roll extrusion coating using a structured cooling roll

DEFF Research Database (Denmark)

Murthy, Swathi; Pranov, Henrik; Pedersen, Henrik Chresten

2016-01-01

This paper investigates a novel, very high throughput, roll-to-roll (R2R) process for nanostructuring of polymer foils, called R2R extrusion coating. It has the potential to accelerate the integration of nanostructured materials in consumer products for a variety of applications, including optical....../height of 100 nm. The best replication was achieved in polypropylene, by running at high roller line-speed of 60 m/min, and high cooling roller temperature of 70°C. Replication in other common polymers like polyethylene and polystyrene was not possible for the parameter range used for the investigation......., technical, and functional surfaces and devices. In roll-to-roll extrusion coating, a molten polymer film is extruded through a flat die forming a melt curtain, and then laminated onto a carrier foil. The lamination occurs as the melt curtain is pressed between a cooling roller and a counter roller...

Roll-to-Roll Nanoforming of Metals Using Laser-Induced Superplasticity.

Science.gov (United States)

Goswami, Debkalpa; Munera, Juan C; Pal, Aniket; Sadri, Behnam; Scarpetti, Caio Lui P G; Martinez, Ramses V

2018-05-24

This Letter describes a low-cost, scalable nanomanufacturing process that enables the continuous forming of thin metallic layers with nanoscale accuracy using roll-to-roll, laser-induced superplasticity (R2RLIS). R2RLIS uses a laser shock to induce the ultrahigh-strain-rate deformation of metallic films at room temperature into low-cost polymeric nanomolds, independently of the original grain size of the metal. This simple and inexpensive nanoforming method does not require access to cleanrooms and associated facilities, and can be easily implemented on conventional CO 2 lasers, enabling laser systems commonly used for rapid prototyping or industrial cutting and engraving to fabricate uniform and three-dimensional crystalline metallic nanostructures over large areas. Tuning the laser power during the R2RLIS process enables the control of the aspect ratio and the mechanical and optical properties of the fabricated nanostructures. This roll-to-roll technique successfully fabricates mechanically strengthened gold plasmonic nanostructures with aspect ratios as high as 5 that exhibit high oxidation resistance and strong optical field enhancements. The CO 2 laser used in R2RLIS can also integrate the fabricated nanostructures on transparent flexible substrates with robust interfacial contact. The ability to fabricate ultrasmooth metallic nanostructures using roll-to-roll manufacturing enables the large scale production, at a relatively low-cost, of flexible plasmonic devices toward emerging applications.

Rolling into spatial disorientation: Simulator demonstration of the post-roll (Gillingham) illusion

NARCIS (Netherlands)

Nooij, S.A.E.; Groen, E.L.

2011-01-01

Introduction: Spatial disorientation (SD) is still a contributing factor in many aviation accidents, stressing the need for adequate SD training scenarios. In this article we focused on the post-roll effect (the sensation of rolling back after a roll maneuver, such as an entry of a coordinated turn)

Research on the rolling moment in the symmetrical and asymmetrical rolling process

Science.gov (United States)

Alexa, V.; Raţiu, S. A.; Kiss, I.; Cioată, C. G.

2017-01-01

Research distribution the rolling moments symmetrical and asymmetrical report presents great importance both in theory and to introduce clarifications to the calculation of rolling resistance line assemblies. Clarifying individuals of metallic material deformation between the rolls single cylinder diameters act of any difference of work and analysis of advance and delay phenomena. Torque drive value for each of the rolling cylinders was done by reducing the thickness of the laminate samples, an experimental facility located in the laboratory of plastic deformation of the Faculty of Engineering Hunedoara. The analysis of research results show that in terms of power consumption for deformation and safety equipment in operation is rational for mills which require such a difference between the work rolls to execute about one cylinder operated.

Teaduseksperiment tõi maailmalõpu paanika / Heiki Suurkask

Index Scriptorium Estoniae

Suurkask, Heiki, 1972-

2008-01-01

Euroopa tuumauuringute organisatsiooni (CERN) ehitatud suur hadronite põrgataja (LHC) alustab täna tööd, läkitades prootonkiired esmakordselt 27-kilomeetrisse maaalusesse ringtunnelisse. Lisaks joonis: Ajalooline eksperiment : "Suure paugu" päev

Dimensional ranges and rolling efficiency in a tandem cold rolling mill

Energy Technology Data Exchange (ETDEWEB)

Larkiola, J.

1997-12-31

In this work, physical models and a neural network theory have been combined in order to predict the properties of a steel strip and to optimise the process parameters in cold rolling. The prediction of the deformation resistance of the material and the friction parameter is based on the physical model presented by Bland, Ford and Ellis and artificial neural network computing (ANN). The accuracy of these models has been tested and proved by using a large amount of the measured data. With the aid of these models it has been shown that (a) the small change to the relative reduction distribution can have a clear effect upon the rolling efficiency, (b) the dimensional ranges of the tandem cold roll mill can be determined and optimised and (c) the possibility to cold roll a new product of new width, strength or thickness can be determined and the parameters of the tandem cold rolling process can be optimised. (orig.) 43 refs.

Cross-linking of L5 protein to 5 S RNA in rat liver 60-S subunits by ultraviolet irradiation

International Nuclear Information System (INIS)

Terao, K.; Uchiumi, T.; Ogata, K.

1980-01-01

After rat liver 60-S ribosomal subunits were irradiated with ultraviolet light at 254 nm, they were treated with EDTA and then subjected to sucrose density-gradient centrifugation to isolate 5 S RNA-protein complex. When 5 S RNA-protein was analyzed by SDS-acrylamide gel electrophoresis which dissociated noncovalent 5 S RNA-protein, two protein bands were observed. The one showed a slower mobility than the protein band (L5) of 5 S RNA-protein from non-irradiated 60 S subunit and the other showed the same mobility as L5 protein. Since the former band was shown to be specific to ultraviolet-irradiation, it was considered as cross-linked 5 S RNA-protein. After the two protein bands were iodinated with 125 I, labeled protein was extracted and treated with RNAase. Thereafter, it was analyzed by two-dimensional acrylamide gel electrophoresis, followed by autoradiography. The results indicate that the protein component of cross-linked 5 S RNA-protein is L5 protein (ribosomal protein); these proteins are designated according to the proposed uniform nomenclature. (Auth.)

The development of clinical activity in relapsing-remitting MS is associated with a decrease of FasL mRNA and an increase of Fas mRNA in peripheral blood

NARCIS (Netherlands)

Lopatinskaya, L.; Boxel van-Dezaire, A.H.H.; Barkhof, F.; Polman, C.H.; Lucas, C.J.; Nagelkerken, L.

2003-01-01

In this longitudinal study, we examined the expression of Fas, FasL, CCR3, CCR5 and CXCR3 mRNA in peripheral blood mononuclear cells (PBMCs) of secondary progressive (SP) and relapsing-remitting (RR) multiple sclerosis (MS) patients. In RR patients, FasL, CCR3 and CCR5 mRNA levels were increased

Unravelling the complexity of microRNA-mediated gene regulation in black pepper (Piper nigrum L.) using high-throughput small RNA profiling.

Science.gov (United States)

Asha, Srinivasan; Sreekumar, Sweda; Soniya, E V

2016-01-01

Analysis of high-throughput small RNA deep sequencing data, in combination with black pepper transcriptome sequences revealed microRNA-mediated gene regulation in black pepper ( Piper nigrum L.). Black pepper is an important spice crop and its berries are used worldwide as a natural food additive that contributes unique flavour to foods. In the present study to characterize microRNAs from black pepper, we generated a small RNA library from black pepper leaf and sequenced it by Illumina high-throughput sequencing technology. MicroRNAs belonging to a total of 303 conserved miRNA families were identified from the sRNAome data. Subsequent analysis from recently sequenced black pepper transcriptome confirmed precursor sequences of 50 conserved miRNAs and four potential novel miRNA candidates. Stem-loop qRT-PCR experiments demonstrated differential expression of eight conserved miRNAs in black pepper. Computational analysis of targets of the miRNAs showed 223 potential black pepper unigene targets that encode diverse transcription factors and enzymes involved in plant development, disease resistance, metabolic and signalling pathways. RLM-RACE experiments further mapped miRNA-mediated cleavage at five of the mRNA targets. In addition, miRNA isoforms corresponding to 18 miRNA families were also identified from black pepper. This study presents the first large-scale identification of microRNAs from black pepper and provides the foundation for the future studies of miRNA-mediated gene regulation of stress responses and diverse metabolic processes in black pepper.

Roll-to-roll UV imprint lithography for flexible electronics

NARCIS (Netherlands)

Maury, P.; Turkenburg, D.H.; Stroeks, N.; Giesen, P.; Barbu, I.; Meinders, E.R.; Bremen, A. van; Iosad, N.; Werf, R. van der; Onvlee, H.

2011-01-01

We propose a roll-to-roll UV imprint lithography tool as a way to pattern flexible PET foil with µm-resolution. As a way to overcome dimensional instability of the foil and its effect on overlay, a self-align approach was investigated, that permits to make several layers in a single lithography

Inhibition of nitric oxide synthesis enhances leukocyte rolling and adhesion in human microvasculature

Directory of Open Access Journals (Sweden)

Hossain Mokarram

2012-07-01

Full Text Available Abstract Background Nitric oxide (NO is a multifunctional signaling molecule that regulates important cellular events in inflammation including leukocyte recruitment. Previous studies have shown that pharmacological inhibition of NO synthesis induces leukocyte recruitment in various in vitro and animal models. However, it is not known whether NO modulation has similar effects on leukocyte-endothelial cell interactions within the human microvasculature. The present study explored the effect of systemic L-NAME treatment on leukocyte recruitment in the SCID-hu mouse model. Methods Human skin xenografts were transplanted in SCID mice to study human leukocyte dynamics in human vasculature. Early events of human leukocyte recruitment in human vasculature were studied using intravital microscopy. NO synthesis was pharmacologically inhibited using NG-nitro-L-arginine methyl ester (L-NAME. Immunohistochemical analysis was performed to elucidate E-selectin expression in human xenograft skin. Human neutrophil-endothelial cell interactions were also studied in an in vitro flow chamber assay system. P- and E-selectin expression on cultured human umbilical vein endothelial cells (HUVECs was measured using ELISA. Platelet-activating factor (PAF synthesis was detected using a TLC-based assay. Results L-NAME treatment significantly enhanced the rolling and adhesion of human leukocytes to the human vasculature. Functional blocking of P- and E-selectins significantly inhibited rolling but not adhesion induced by inhibition of NO synthesis. Systemic L-NAME treatment enhanced E-selectin expression in human xenograft skin. L-NAME treatment significantly enhanced P- and E-selectin expression on HUVECs. L-NAME treatment did not significantly modify neutrophil rolling or adhesion to HUVECs indicating that L-NAME−induced subtle P- and E-selectin expression was insufficient to elicit dynamic neutrophil-HUVEC interactions in vitro. Moreover, synthesis of endothelial

Strategic flat rolling of Ag/BSCCO-2223 tapes

DEFF Research Database (Denmark)

Nielsen, Morten Storgård; Bech, Jakob Ilsted; Eriksen, Morten

2002-01-01

In the process of single-step flat rolling of multifilament Ag/BSCCO-2223 wire to tape previous work has shown the optimum strategy giving maximum critical current density to be it balance between the length and width strain, so they are of equal size i.e. so, that the logarithmic strain ratio, L...

EKA - suur tükk ajab suu lõhki / Tuuli Jõesaar

Index Scriptorium Estoniae

Jõesaar, Tuuli

2011-01-01

Eesti Kunstiakadeemia uue hoone arhitektuurikonkursi võidutöö "Art Plaza" projekti muutmisest. Kadunud on korruseid läbinud avatud aatrium. Uuenenud plaanid ei ole veel EKA nõukogu ette jõudnud. Projektis saab muudatusi teha töö autor (Taani bürood SEA ja Effekt koos AET Arhitektide ja Allianss Arhitektidega) koostöös dekaanide, õppejõudude ja tehniliste ekspertidega. Lisandunud on tudengite algatusrühm

On the constant-roll inflation

Science.gov (United States)

Yi, Zhu; Gong, Yungui

2018-03-01

The primordial power spectra of scalar and tensor perturbations during slow-roll inflation are usually calculated with the method of Bessel function approximation. For constant-roll or ultra slow-roll inflation, the method of Bessel function approximation may be invalid. We compare the numerical results with the analytical results derived from the Bessel function approximation, and we find that they differ significantly on super-horizon scales if the constant slow-roll parameter ηH is not small. More accurate method is needed for calculating the primordial power spectrum for constant-roll inflation.

Effect of Lactobacillus casei- casei and Lactobacillus reuteri on acrylamide formation in flat bread and Bread roll.

Science.gov (United States)

Dastmalchi, Farnaz; Razavi, Seyed Hadi; Faraji, Mohammad; Labbafi, Mohsen

2016-03-01

The aim of this study was the evaluation of fermentation by lactic acid bacteria (LAB) contains lactobacillus (L.) casei- casei and L. reuteri on acrylamide formation and physicochemical properties of the Iranian flat bread named, Sangak, and Bread roll. Sangak and Bread roll were made with whole and white wheat flour, respectively. Whole-wheat flour had upper content of protein, sugar, ash, fiber, damaged starch and the activity of amylase than the white wheat flour. After 24 h of fermentation, the pH values of the sourdoughs made from whole-wheat flour (3.00, 2.90) were lower, in compared to sourdoughs prepared from white wheat flour (3.60, 3.58). In addition, in Sangak bread, glucose, and fructose were completely utilized after fermentation, but in bread roll, the reduced sugar levels increased after fermentation and baking that represent microorganisms cannot be activated and utilized sugars. Acrylamide formation was impacted by pH of sourdough and total reducing sugar (r = 0.915, r = 0.885 respectively). Bread roll and Sangak bread were fermented by L. casei- casei contained lowest acrylamide content, in two bread types (219.1, 104.3 μg/kg respectively). As an important result, the acrylamide content of Sangak bread in all cases was lower than in the Bread roll.

Technology development for roll-to-roll production of organic photovoltaics

NARCIS (Netherlands)

Galagan, Y.O.; Vries, I.G. de; Langen, A.P.; Andriessen, H.A.J.M.; Verhees, W.J.H.; Veenstra, S.C.; Kroon, J.M.

2011-01-01

In order to reach the objective of low-cost, large area organic photovoltaic systems, we build up a knowledge base concerning the influence of process conditions on the performance of polymer solar cells. A large area solar cell module, with roll-to-roll coated PEDOT:PSS and photoactive layers

Rolling Force Prediction in Heavy Plate Rolling Based on Uniform Differential Neural Network

Directory of Open Access Journals (Sweden)

Fei Zhang

2016-01-01

Full Text Available Accurate prediction of the rolling force is critical to assuring the quality of the final product in steel manufacturing. Exit thickness of plate for each pass is calculated from roll gap, mill spring, and predicted roll force. Ideal pass scheduling is dependent on a precise prediction of the roll force in each pass. This paper will introduce a concept that allows obtaining the material model parameters directly from the rolling process on an industrial scale by the uniform differential neural network. On the basis of the characteristics that the uniform distribution can fully characterize the solution space and enhance the diversity of the population, uniformity research on differential evolution operator is made to get improved crossover with uniform distribution. When its original function is transferred with a transfer function, the uniform differential evolution algorithms can quickly solve complex optimization problems. Neural network structure and weights threshold are optimized by uniform differential evolution algorithm, and a uniform differential neural network is formed to improve rolling force prediction accuracy in process control system.

Apoptosis Triggers Specific, Rapid, and Global mRNA Decay with 3′ Uridylated Intermediates Degraded by DIS3L2

Directory of Open Access Journals (Sweden)

Marshall P. Thomas

2015-05-01

Full Text Available Apoptosis is a tightly coordinated cell death program that damages mitochondria, DNA, proteins, and membrane lipids. Little is known about the fate of RNA as cells die. Here, we show that mRNAs, but not noncoding RNAs, are rapidly and globally degraded during apoptosis. mRNA decay is triggered early in apoptosis, preceding membrane lipid scrambling, genomic DNA fragmentation, and apoptotic changes to translation initiation factors. mRNA decay depends on mitochondrial outer membrane permeabilization and is amplified by caspase activation. 3′ truncated mRNA decay intermediates with nontemplated uridylate-rich tails are generated during apoptosis. These tails are added by the terminal uridylyl transferases (TUTases ZCCHC6 and ZCCHC11, and the uridylated transcript intermediates are degraded by the 3′ to 5′ exonuclease DIS3L2. Knockdown of DIS3L2 or the TUTases inhibits apoptotic mRNA decay, translation arrest, and cell death, whereas DIS3L2 overexpression enhances cell death. Our results suggest that global mRNA decay is an overlooked hallmark of apoptosis.

Finite-element model to predict roll-separation force and defects during rolling of U-10Mo alloys

Science.gov (United States)

Soulami, Ayoub; Burkes, Douglas E.; Joshi, Vineet V.; Lavender, Curt A.; Paxton, Dean

2017-10-01

A major goal of the Convert Program of the U.S. Department of Energy's National Nuclear Security Administration (DOE/NNSA) is to enable high-performance research reactors to operate with low-enriched uranium rather than the high-enriched uranium currently used. To this end, uranium alloyed with 10 wt% molybdenum (U-10Mo) represents an ideal candidate because of its stable gamma phase, low neutron caption cross section, acceptable swelling response, and predictable irradiation behavior. However, because of the complexities of the fuel design and the need for rolled monolithic U-10Mo foils, new developments in processing and fabrication are necessary. This study used a finite-element code, LS-DYNA, as a predictive tool to optimize the rolling process. Simulations of the hot rolling of U-10Mo coupons encapsulated in low-carbon steel were conducted following two different schedules. Model predictions of the roll-separation force and roll pack thicknesses at different stages of the rolling process were compared with experimental measurements. The study reported here discussed various attributes of the rolled coupons revealed by the model (e.g., waviness and thickness non-uniformity like dog-boning). To investigate the influence of the cladding material on these rolling defects, other cases were simulated: hot rolling with alternative can materials, namely, 304 stainless steel and Zircaloy-2, and bare-rolling. Simulation results demonstrated that reducing the mismatch in strength between the coupon and can material improves the quality of the rolled sheet. Bare-rolling simulation results showed a defect-free rolled coupon. The finite-element model developed and presented in this study can be used to conduct parametric studies of several process parameters (e.g., rolling speed, roll diameter, can material, and reduction).

75 FR 42782 - Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil, Japan, and Russia

Science.gov (United States)

2010-07-22

...)] Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil, Japan, and Russia AGENCY: United... Brazil and Japan, and the suspended investigation on hot-rolled steel from Russia. SUMMARY: The... Japan, and the suspended investigation on hot-rolled steel from Russia would be likely to lead to...

75 FR 62566 - Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil, Japan, and Russia

Science.gov (United States)

2010-10-12

...)] Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil, Japan, and Russia AGENCY: United... antidumping duty investigation on hot-rolled steel from Russia. SUMMARY: The Commission hereby gives notice of... suspended investigation on hot-rolled steel from Russia would be likely to lead to continuation or...

Yeast polypeptide exit tunnel ribosomal proteins L17, L35 and L37 are necessary to recruit late-assembling factors required for 27SB pre-rRNA processing.

Science.gov (United States)

Gamalinda, Michael; Jakovljevic, Jelena; Babiano, Reyes; Talkish, Jason; de la Cruz, Jesús; Woolford, John L

2013-02-01

Ribosome synthesis involves the coordinated folding and processing of pre-rRNAs with assembly of ribosomal proteins. In eukaryotes, these events are facilitated by trans-acting factors that propel ribosome maturation from the nucleolus to the cytoplasm. However, there is a gap in understanding how ribosomal proteins configure pre-ribosomes in vivo to enable processing to occur. Here, we have examined the role of adjacent yeast r-proteins L17, L35 and L37 in folding and processing of pre-rRNAs, and binding of other proteins within assembling ribosomes. These three essential ribosomal proteins, which surround the polypeptide exit tunnel, are required for 60S subunit formation as a consequence of their role in removal of the ITS2 spacer from 27SB pre-rRNA. L17-, L35- and L37-depleted cells exhibit turnover of aberrant pre-60S assembly intermediates. Although the structure of ITS2 does not appear to be grossly affected in their absence, these three ribosomal proteins are necessary for efficient recruitment of factors required for 27SB pre-rRNA processing, namely, Nsa2 and Nog2, which associate with pre-60S ribosomal particles containing 27SB pre-rRNAs. Altogether, these data support that L17, L35 and L37 are specifically required for a recruiting step immediately preceding removal of ITS2.

Roll paper pilot. [mathematical model for predicting pilot rating of aircraft in roll task

Science.gov (United States)

Naylor, F. R.; Dillow, J. D.; Hannen, R. A.

1973-01-01

A mathematical model for predicting the pilot rating of an aircraft in a roll task is described. The model includes: (1) the lateral-directional aircraft equations of motion; (2) a stochastic gust model; (3) a pilot model with two free parameters; and (4) a pilot rating expression that is a function of rms roll angle and the pilot lead time constant. The pilot gain and lead time constant are selected to minimize the pilot rating expression. The pilot parameters are then adjusted to provide a 20% stability margin and the adjusted pilot parameters are used to compute a roll paper pilot rating of the aircraft/gust configuration. The roll paper pilot rating was computed for 25 aircraft/gust configurations. A range of actual ratings from 2 to 9 were encountered and the roll paper pilot ratings agree quite well with the actual ratings. In addition there is good correlation between predicted and measured rms roll angle.

Lengthening the lifetime of roll-to-roll produced polymer solar cells

DEFF Research Database (Denmark)

Madsen, Morten Vesterager

the knowledge of the degradation mechanisms involved in roll-to-roll coated polymer solar cells. While only a part of the experiments have directly involved roll-to-roll coated devices, most of the work is applicable to coated devices. The first part of the dissertation is devoted to the study of in......The field of polymer solar cells is a field with an exponential growth in the number of published papers. It is a field defined by a set of challenges including; efficiency, stability and processability. Before all of these challenges have been addressed; polymer solar cells...... will not be a commercial success. This dissertation is devoted primarily to the study of the stability of polymer solar cells, and more specifically to designing and verifying experimental techniques, procedures, and automated solutions to stability tests and characterization. The goal of the project was to expand...

Oxidized Low-density Lipoprotein (ox-LDL) Cholesterol Induces the Expression of miRNA-223 and L-type Calcium Channel Protein in Atrial Fibrillation

Science.gov (United States)

He, Fengping; Xu, Xin; Yuan, Shuguo; Tan, Liangqiu; Gao, Lingjun; Ma, Shaochun; Zhang, Shebin; Ma, Zhanzhong; Jiang, Wei; Liu, Fenglian; Chen, Baofeng; Zhang, Beibei; Pang, Jungang; Huang, Xiuyan; Weng, Jiaqiang

2016-08-01

Atrial fibrillation (AF) is the most common sustained arrhythmia causing high morbidity and mortality. While changing of the cellular calcium homeostasis plays a critical role in AF, the L-type calcium channel α1c protein has suggested as an important regulator of reentrant spiral dynamics and is a major component of AF-related electrical remodeling. Our computational modeling predicted that miRNA-223 may regulate the CACNA1C gene which encodes the cardiac L-type calcium channel α1c subunit. We found that oxidized low-density lipoprotein (ox-LDL) cholesterol significantly up-regulates both the expression of miRNA-223 and L-type calcium channel protein. In contrast, knockdown of miRNA-223 reduced L-type calcium channel protein expression, while genetic knockdown of endogenous miRNA-223 dampened AF vulnerability. Transfection of miRNA-223 by adenovirus-mediated expression enhanced L-type calcium currents and promoted AF in mice while co-injection of a CACNA1C-specific miR-mimic counteracted the effect. Taken together, ox-LDL, as a known factor in AF-associated remodeling, positively regulates miRNA-223 transcription and L-type calcium channel protein expression. Our results implicate a new molecular mechanism for AF in which miRNA-223 can be used as an biomarker of AF rheumatic heart disease.

Influences of rolling method on deformation force in cold roll-beating forming process

Science.gov (United States)

Su, Yongxiang; Cui, Fengkui; Liang, Xiaoming; Li, Yan

2018-03-01

In process, the research object, the gear rack was selected to study the influence law of rolling method on the deformation force. By the mean of the cold roll forming finite element simulation, the variation regularity of radial and tangential deformation was analysed under different rolling methods. The variation of deformation force of the complete forming racks and the single roll during the steady state under different rolling modes was analyzed. The results show: when upbeating and down beating, radial single point average force is similar, the tangential single point average force gap is bigger, the gap of tangential single point average force is relatively large. Add itionally, the tangential force at the time of direct beating is large, and the dire ction is opposite with down beating. With directly beating, deformation force loading fast and uninstall slow. Correspondingly, with down beating, deformat ion force loading slow and uninstall fast.

75 FR 64246 - Certain Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil: Correction to Notice of...

Science.gov (United States)

2010-10-19

...-Rolled Carbon-Quality Steel Products From Brazil: Correction to Notice of Antidumping Duty Order AGENCY... certain hot-rolled flat-rolled carbon-quality steel products from Brazil. See Antidumping Duty Order: Certain Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil, 67 FR 11093 (March 12, 2002...

Rolling Shutter Motion Deblurring

KAUST Repository

Su, Shuochen

2015-06-07

Although motion blur and rolling shutter deformations are closely coupled artifacts in images taken with CMOS image sensors, the two phenomena have so far mostly been treated separately, with deblurring algorithms being unable to handle rolling shutter wobble, and rolling shutter algorithms being incapable of dealing with motion blur. We propose an approach that delivers sharp and undis torted output given a single rolling shutter motion blurred image. The key to achieving this is a global modeling of the camera motion trajectory, which enables each scanline of the image to be deblurred with the corresponding motion segment. We show the results of the proposed framework through experiments on synthetic and real data.

High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L..

Directory of Open Access Journals (Sweden)

Livia Donaire

Full Text Available Small RNAs (sRNAs of 20 to 25 nucleotides (nt in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has enabled profiling of the sRNA component of more than 40 plant species. Here, we used deep sequencing and molecular methods to report the first inventory of sRNAs in olive (Olea europaea L.. sRNA libraries prepared from juvenile and adult shoots revealed that the 24-nt class dominates the sRNA transcriptome and atypically accumulates to levels never seen in other plant species, suggesting an active role of heterochromatin silencing in the maintenance and integrity of its large genome. A total of 18 known miRNA families were identified in the libraries. Also, 5 other sRNAs derived from potential hairpin-like precursors remain as plausible miRNA candidates. RNA blots confirmed miRNA expression and suggested tissue- and/or developmental-specific expression patterns. Target mRNAs of conserved miRNAs were computationally predicted among the olive cDNA collection and experimentally validated through endonucleolytic cleavage assays. Finally, we use expression data to uncover genetic components of the miR156, miR172 and miR390/TAS3-derived trans-acting small interfering RNA (tasiRNA regulatory nodes, suggesting that these interactive networks controlling developmental transitions are fully operational in olive.

RNA transcription in isolated chloroplasts during senescence and rejuvenation of intact cotyledons of CUCURBITA PEPO L. (ZUCCHINI)

International Nuclear Information System (INIS)

Mishev, K.; Ananiev, E.; Denev, L.; Radeva, G.

2006-01-01

RNA transcription was studied in intact chloroplasts isolated from cotyledons of Cucurbita pepoL. (zucchini) during their growth and development including natural senescence and rejuvenation. Rejuvenation of cotyledons was studied after decapitation of the epicotyl above the senescing yellow cotyledons. Maximal incorporation of [32P] UTP into overall chloroplast RNA was measured two days after exposure of seedlings to light (day 6 th after the onset of germination), followed by a gradual decrease reaching minimal values at the age of 25-28 days when cotyledons began to yellow and eventually die. Rejuvenation of cotyledons completely restored chloroplast RNA synthesis and fifteen days after decapitation (at the age of 40 days), the values of chloroplast transcription even exceeded that of the maximal transcriptional activity in young cotyledons. Inhibitory analysis with tagetitoxin (a specific inhibitor of plastid encoded chloroplast RNA polymerase (PEP)) showed that in young and rejuvenated cotyledons about 85% of chloroplast RNA polymerase activity was due to PEP and only 15% corresponded to the nuclear encoded plastid RNA polymerase (NEP). Definite regions of two chloroplast encoded genes were amplified by means of PCR technique using specific DNA primers for Rubisco large subunit gene (rbcL) and the housekeeping gene for chloroplast 16S rRNA as well as chloroplast DNA as a template. The appropriate lengths of the amplified DNA fragments were checked by restriction analysis

Interlayer adhesion in roll-to-roll processed flexible inverted polymer solar cells

KAUST Repository

Dupont, Stephanie R.; Oliver, Mark; Krebs, Frederik C.; Dauskardt, Reinhold H.

2012-01-01

The interlayer adhesion of roll-to-roll processed flexible inverted P3HT:PCBM bulk heterojunction (BHJ) polymer solar cells is reported. Poor adhesion between adjacent layers may result in loss of device performance from delamination driven

A Coupled Model for Work Roll Thermal Contour with Subsectional Cooling in Aluminum Strip Cold Rolling

Directory of Open Access Journals (Sweden)

Shao Jian

2014-10-01

Full Text Available Little attention had been given to the evaluation of subsectional cooling control ability under complicated working conditions. In this paper, heat generation was calculated by using finite difference method. Strip hardening, work roll elastic deformation and elastic recovery of strip were taken into account. The mean coefficient of convective heat transfer on work roll surface was simulated by FLUENT. Calculation model had used the alternative finite difference scheme, which improved the model stability and computing speed. The simulation result shows that subsectional cooling control ability is different between different rolling passes. Positive and negative control abilities are roughly the same in the same pass. The increase of rolled length, working pressure of header and friction coefficient has positive effect on subsectional cooling control ability, and the rolling speed is on the contrary. On the beginning of the pass, when work roll surface has not reached the stable temperature, control ability of subsectional cooling is mainly affected by rolled length. The effect of mean coefficient of convective heat transfer and coefficient of friction is linear. When rolling speed is over 500 m/min, control ability of subsectional cooling becomes stable.

Relationship between shoulder roll and hand propulsion in the front crawl stroke.

Science.gov (United States)

Kudo, Shigetada; Sakurai, Yoshihisa; Miwa, Takahiro; Matsuda, Yuji

2017-05-01

This study re-evaluated the magnitude of hand propulsion (HP) in the pull and push phases of the front crawl stroke and investigated the association between the angular velocity of shoulder roll (ω SR ) and hand propulsive lift (HP L ). ω SR was computed in the plane normal to a forward direction for 16 skilled swimmers performing the front crawl stroke at a maximal sprinting pace. HP, hand propulsive drag (HP D ) and HP L were determined by a dynamic pressure approach. HP and HP D in the pull phase were greater than in the push phase (P push phase. Eleven swimmers out of the 16 swimmers had a significant within-swimmers correlation between ω SR and HP L in the push phase (P push phase as the ω SR of rolling back to the neutral position became faster. A swimmer should use more drag for hand propulsion in the pull phase and propulsion from drag and lift equally in the push phase. Based on the relationship between ω SR and HP L in the push phase, a possible stroke technique to enhance HP L using ω SR is discussed.

Roll-to-roll printed silver nanowires for increased stability of flexible ITO-free organic solar cell modules

DEFF Research Database (Denmark)

Benatto, Gisele Alves dos Reis; Roth, Bérenger; Corazza, Michael

2016-01-01

We report the use of roll-to-roll printed silver nanowire networks as front electrodes for fully roll-to-roll processed flexible indium-tin-oxide (ITO) free OPV modules. We prepared devices with two types of back electrodes, a simple PEDOT:PSS back electrode and a PEDOT:PSS back electrode...

Fabrication of Superhydrophobic Metallic Surface by Wire Electrical Discharge Machining for Seamless Roll-to-Roll Printing

Directory of Open Access Journals (Sweden)

Jin-Young So

2018-04-01

Full Text Available This paper presents a proposal of a direct one-step method to fabricate a multi-scale superhydrophobic metallic seamless roll mold. The mold was fabricated using the wire electrical discharge machining (WEDM technique for a roll-to-roll imprinting application to produce a large superhydrophobic surface. Taking advantage of the exfoliating characteristic of the metallic surface, nano-sized surface roughness was spontaneously formed while manufacturing the micro-sized structure: that is, a dual-scale hierarchical structure was easily produced in a simple one-step fabrication with a large area on the aluminum metal surface. This hierarchical structure showed superhydrophobicity without chemical coating. A roll-type seamless mold for the roll-to-roll process was fabricated through engraving the patterns on the cylindrical substrate, thereby enabling to make a continuous film with superhydrophobicity.

Rolling Resistance Measurement and Model Development

DEFF Research Database (Denmark)

Andersen, Lasse Grinderslev; Larsen, Jesper; Fraser, Elsje Sophia

2015-01-01

There is an increased focus worldwide on understanding and modeling rolling resistance because reducing the rolling resistance by just a few percent will lead to substantial energy savings. This paper reviews the state of the art of rolling resistance research, focusing on measuring techniques, s......, surface and texture modeling, contact models, tire models, and macro-modeling of rolling resistance...

RNA-binding proteins ZFP36L1 and ZFP36L2 promote cell quiescence.

Science.gov (United States)

Galloway, Alison; Saveliev, Alexander; Łukasiak, Sebastian; Hodson, Daniel J; Bolland, Daniel; Balmanno, Kathryn; Ahlfors, Helena; Monzón-Casanova, Elisa; Mannurita, Sara Ciullini; Bell, Lewis S; Andrews, Simon; Díaz-Muñoz, Manuel D; Cook, Simon J; Corcoran, Anne; Turner, Martin

2016-04-22

Progression through the stages of lymphocyte development requires coordination of the cell cycle. Such coordination ensures genomic integrity while cells somatically rearrange their antigen receptor genes [in a process called variable-diversity-joining (VDJ) recombination] and, upon successful rearrangement, expands the pools of progenitor lymphocytes. Here we show that in developing B lymphocytes, the RNA-binding proteins (RBPs) ZFP36L1 and ZFP36L2 are critical for maintaining quiescence before precursor B cell receptor (pre-BCR) expression and for reestablishing quiescence after pre-BCR-induced expansion. These RBPs suppress an evolutionarily conserved posttranscriptional regulon consisting of messenger RNAs whose protein products cooperatively promote transition into the S phase of the cell cycle. This mechanism promotes VDJ recombination and effective selection of cells expressing immunoglobulin-μ at the pre-BCR checkpoint. Copyright © 2016, American Association for the Advancement of Science.

Rolled lawn as tool for industrial barren remediation

Science.gov (United States)

Gorbacheva, T. T.; Ivanova, L. A.; Kikuchi, R.; Gerardo, R.

2009-04-01

Fast development of the industrial and urban territories during last century has led to great disturbance of natural ecosystems in a lot of regions of the world. In the Far North the risk resulted from technogenic influence involves essentially more expressed negative consequences for the nature comparing to a regions of averages and southern latitudes due to higher sensitivity of northern ecosystems. Since thirtieth years of last century industrial complexes on extraction and processing of nonferrous metals ores are functioned on Kola peninsula territory. They are powerful sources of emissions of acidifying substances and heavy metals. Long term influence of these emissions resulted in deep degradation of terrestrial ecosystems up to industrial barren arising in immediate proximity to industrial centre Monchegorsk. The most radical way of disturbed territories rehabilitation is biological remediation. In 2006-2008 innovative methods of high-quality grass cover performance was developed in local enterprise «VIPON» in Apatity. Vermiculite trademark «VIPON» is characterized by not broken structure of minerals combined with week reactance, high mechanical durability, favorable рН equal 6.5-7.0, valuable absorptive and ion exchange properties. Final product of proposed technology was rolled lawn which successfully applied for remediation of disturbed sites in urban territories as such as industrial plots with low contamination. One of abstract authors namely L.Ivanova is one of technology implementators. During 2008 the field test was performed near the smelter complex (67°51'N, 32°48'E) to estimate suitability of proposed method for site remediation in more severe conditions such as in industrial barren. The method is based on cultivation of perennial grasses using hydroponics with thermally inflated vermiculite from local deposit (Kovdor) followed by rolled lawn placement on very contaminated sites near Monchegorsk. Great advantage of rolled lawn is short

Computational design of rolling bearings

CERN Document Server

Nguyen-Schäfer, Hung

2016-01-01

This book comprehensively presents the computational design of rolling bearings dealing with many interdisciplinary difficult working fields. They encompass elastohydrodynamics (EHD), Hertzian contact theory, oil-film thickness in elastohydrodynamic lubrication (EHL), bearing dynamics, tribology of surface textures, fatigue failure mechanisms, fatigue lifetimes of rolling bearings and lubricating greases, Weibull distribution, rotor balancing, and airborne noises (NVH) in the rolling bearings. Furthermore, the readers are provided with hands-on essential formulas based on the up-to-date DIN ISO norms and helpful examples for computational design of rolling bearings. The topics are intended for undergraduate and graduate students in mechanical and material engineering, research scientists, and practicing engineers who want to understand the interactions between these working fields and to know how to design the rolling bearings for automotive industry and many other industries.

Microstructural and mechanical responses to various rolling speeds determined in multi-pass break-down rolling of AZ31B alloy

Science.gov (United States)

Jia, Weitao; Tang, Yan; Ning, Fangkun; Le, Qichi; Cui, Jianzhong

2018-04-01

Different rolling operations of as-cast AZ31B alloy were performed under different rolling speed (18 ∼ 72 m min‑1) and rolling pass conditions at 400 °C. Microstructural studies, tensile testing and formability evaluation relevant to each rolling operation were investigated. For 1-pass rolling, coarse average grain size (CAGS) region gradually approached the center layer as the rolling speed increased. Moreover, twins, shear bands and coarse-grain structures were the dominant components in the microstructure of plates rolled at 18, 48 and 72 m min‑1, respectively, indicating the severe deformation inhomogeneity under the high reduction per pass condition. For 2-pass rolling and 4-pass rolling, dynamic recrystallization was observed to be well and CAGS region has substantially disappeared, indicating the significant improvement in deformation uniformity and further the grain homogenization under the conditions. Microstructure uniformity degree of 2-pass rolled plates did not vary much as the rolling speed varied. On this basis, shear band distribution dominated the deformation behavior during the uniaxial tension of the 2-pass rolled plates. However, microstructure uniformity accompanied by twin distribution played a leading role in stretching the 4-pass rolled plates.

75 FR 77828 - Certain Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil: Extension of Time Limit...

Science.gov (United States)

2010-12-14

...-Rolled Carbon-Quality Steel Products From Brazil: Extension of Time Limit for Final Results of...-Rolled Carbon Quality Steel Products From Brazil: Preliminary Results of Countervailing Duty... administrative review of the countervailing duty order on certain hot-rolled flat-rolled carbon- quality steel...

Multilength Scale Patterning of Functional Layers by Roll-to-Roll Ultraviolet-Light-Assisted Nanoimprint Lithography.

Science.gov (United States)

Leitgeb, Markus; Nees, Dieter; Ruttloff, Stephan; Palfinger, Ursula; Götz, Johannes; Liska, Robert; Belegratis, Maria R; Stadlober, Barbara

2016-05-24

Top-down fabrication of nanostructures with high throughput is still a challenge. We demonstrate the fast (>10 m/min) and continuous fabrication of multilength scale structures by roll-to-roll UV-nanoimprint lithography on a 250 mm wide web. The large-area nanopatterning is enabled by a multicomponent UV-curable resist system (JRcure) with viscous, mechanical, and surface properties that are tunable over a wide range to either allow for usage as polymer stamp material or as imprint resist. The adjustable elasticity and surface chemistry of the resist system enable multistep self-replication of structured resist layers. Decisive for defect-free UV-nanoimprinting in roll-to-roll is the minimization of the surface energies of stamp and resist, and the stepwise reduction of the stiffness from one layer to the next is essential for optimizing the reproduction fidelity especially for nanoscale features. Accordingly, we demonstrate the continuous replication of 3D nanostructures and the high-throughput fabrication of multilength scale resist structures resulting in flexible polyethylenetherephtalate film rolls with superhydrophobic properties. Moreover, a water-soluble UV-imprint resist (JRlift) is introduced that enables residue-free nanoimprinting in roll-to-roll. Thereby we could demonstrate high-throughput fabrication of metallic patterns with only 200 nm line width.

Control of surface thermal scratch of strip in tandem cold rolling

Science.gov (United States)

Chen, Jinshan; Li, Changsheng

2014-07-01

The thermal scratch seriously affects the surface quality of the cold rolled stainless steel strip. Some researchers have carried out qualitative and theoretical studies in this field. However, there is currently a lack of research on effective forecast and control of thermal scratch defects in practical production, especially in tandem cold rolling. In order to establish precise mathematical model of oil film thickness in deformation zone, the lubrication in cold rolling process of SUS410L stainless steel strip is studied, and major factors affecting oil film thickness are also analyzed. According to the principle of statistics, mathematical model of critical oil film thickness in deformation zone for thermal scratch is built, with fitting and regression analytical method, and then based on temperature comparison method, the criterion for deciding thermal scratch defects is put forward. Storing and calling data through SQL Server 2010, a software on thermal scratch defects control is developed through Microsoft Visual Studio 2008 by MFC technique for stainless steel in tandem cold rolling, and then it is put into practical production. Statistics indicate that the hit rate of thermal scratch is as high as 92.38%, and the occurrence rate of thermal scratch is decreased by 89.13%. Owing to the application of the software, the rolling speed is increased by approximately 9.3%. The software developed provides an effective solution to the problem of thermal scratch defects in tandem cold rolling, and helps to promote products surface quality of stainless steel strips in practical production.

Material-Process-Performance Relationships for Roll-to-Roll Coated PEM Electrodes

Energy Technology Data Exchange (ETDEWEB)

Mauger, Scott; Neyerlin, K.C.; Stickel, Jonathan; Ulsh, Michael; More, Karren; Wood, David

2017-04-26

Roll-to-roll (R2R) coating is the most economical and highest throughput method for producing fuel cell electrodes. R2R coating encompasses many different methodologies to create uniform films on a moving web substrate. Here we explore two coating methods, gravure and slot die, to understand the impacts of each on film uniformity and performance.

Constant-roll (quasi-)linear inflation

Science.gov (United States)

Karam, A.; Marzola, L.; Pappas, T.; Racioppi, A.; Tamvakis, K.

2018-05-01

In constant-roll inflation, the scalar field that drives the accelerated expansion of the Universe is rolling down its potential at a constant rate. Within this framework, we highlight the relations between the Hubble slow-roll parameters and the potential ones, studying in detail the case of a single-field Coleman-Weinberg model characterised by a non-minimal coupling of the inflaton to gravity. With respect to the exact constant-roll predictions, we find that assuming an approximate slow-roll behaviour yields a difference of Δ r = 0.001 in the tensor-to-scalar ratio prediction. Such a discrepancy is in principle testable by future satellite missions. As for the scalar spectral index ns, we find that the existing 2-σ bound constrains the value of the non-minimal coupling to ξphi ~ 0.29–0.31 in the model under consideration.

Rolling of molybdenum and niobium tubes on cold-rolling mill with high stiff stand

Energy Technology Data Exchange (ETDEWEB)

Potapov, I N; Shejkh-Ali, A D; Filimonov, G V; Lunev, A G

1984-03-01

To develop the technique of tube production the process of rolling is studied and comparative evaluation of the structure formed is carried out. It is shown that billets of rods deformed by screw rolling have the improved plastic properties and are deformed on cold-rolling mill (CRM) with a high degree of reduction without defect formation. High stiff stand of the CRM permits to produce high-quality molybdenum tubes.

Effect of microstructural anisotropy on fracture toughness of hot rolled 13Cr ODS steel - The role of primary and secondary cracking

Science.gov (United States)

Das, A.; Viehrig, H. W.; Bergner, F.; Heintze, C.; Altstadt, E.; Hoffmann, J.

2017-08-01

ODS steels have been known to exhibit anisotropic fracture behaviour and form secondary cracks. In this work, the factors responsible for the anisotropic fracture behaviour have been investigated using scanning electron microscopy and electron backscatter microscopy. Fracture toughness of hot rolled 13Cr ODS steel was determined using unloading compliance method for L-T and T-L orientations at various temperatures. L-T orientation had higher fracture toughness than T-L orientation and also contained more pronounced secondary cracking. Secondary cracks appeared at lower loads than primary cracks in both orientations. Primary crack propagation was found to be preferentially through fine grains in a bimodal microstructure. Grains were aligned and elongated the most towards rolling direction followed by T and S directions resulting in fracture anisotropy. Crystallographic texture and preferential alignment of Ti enriched particles parallel to rolling direction also contributed towards fracture anisotropy.

Lowering the quantification limit of the QubitTM RNA HS assay using RNA spike-in.

Science.gov (United States)

Li, Xin; Ben-Dov, Iddo Z; Mauro, Maurizio; Williams, Zev

2015-05-06

RNA quantification is often a prerequisite for most RNA analyses such as RNA sequencing. However, the relatively low sensitivity and large sample consumption of traditional RNA quantification methods such as UV spectrophotometry and even the much more sensitive fluorescence-based RNA quantification assays, such as the Qubit™ RNA HS Assay, are often inadequate for measuring minute levels of RNA isolated from limited cell and tissue samples and biofluids. Thus, there is a pressing need for a more sensitive method to reliably and robustly detect trace levels of RNA without interference from DNA. To improve the quantification limit of the Qubit™ RNA HS Assay, we spiked-in a known quantity of RNA to achieve the minimum reading required by the assay. Samples containing trace amounts of RNA were then added to the spike-in and measured as a reading increase over RNA spike-in baseline. We determined the accuracy and precision of reading increases between 1 and 20 pg/μL as well as RNA-specificity in this range, and compared to those of RiboGreen(®), another sensitive fluorescence-based RNA quantification assay. We then applied Qubit™ Assay with RNA spike-in to quantify plasma RNA samples. RNA spike-in improved the quantification limit of the Qubit™ RNA HS Assay 5-fold, from 25 pg/μL down to 5 pg/μL while maintaining high specificity to RNA. This enabled quantification of RNA with original concentration as low as 55.6 pg/μL compared to 250 pg/μL for the standard assay and decreased sample consumption from 5 to 1 ng. Plasma RNA samples that were not measurable by the Qubit™ RNA HS Assay were measurable by our modified method. The Qubit™ RNA HS Assay with RNA spike-in is able to quantify RNA with high specificity at 5-fold lower concentration and uses 5-fold less sample quantity than the standard Qubit™ Assay.

Kulturens rolle

DEFF Research Database (Denmark)

Hasse, Cathrine

2007-01-01

Kulturens rolle. Herunder kulturens betydning for psykologisk teori og forskning set i lyset af den stigende globalisering og væksten i kulturmøder. Der gives eksempler fra hverdagssituationer, den pædagogiske praksis, fra indvandrerforskning, turister men også fra avisernes referater af kulturmø......Kulturens rolle. Herunder kulturens betydning for psykologisk teori og forskning set i lyset af den stigende globalisering og væksten i kulturmøder. Der gives eksempler fra hverdagssituationer, den pædagogiske praksis, fra indvandrerforskning, turister men også fra avisernes referater af...

Ribosomal protein L5 has a highly twisted concave surface and flexible arms responsible for rRNA binding.

OpenAIRE

Nakashima, T; Yao, M; Kawamura, S; Iwasaki, K; Kimura, M; Tanaka, I

2001-01-01

Ribosomal protein L5 is a 5S rRNA binding protein in the large subunit and plays an essential role in the promotion of a particular conformation of 5S rRNA. The crystal structure of the ribosomal protein L5 from Bacillus stearothermophilus has been determined at 1.8 A resolution. The molecule consists of a five-stranded antiparallel beta-sheet and four alpha-helices, which fold in a way that is topologically similar to the ribonucleoprotein (RNP) domain. The molecular shape and electrostatic ...

Finite-element model to predict roll-separation force and defects during rolling of U-10Mo alloys

Energy Technology Data Exchange (ETDEWEB)

Soulami, Ayoub; Burkes, Douglas E.; Joshi, Vineet V.; Lavender, Curt A.; Paxton, Dean

2017-10-01

This study used a finite element code, LSDYNA, as a predictive tool to optimize the rolling process. Simulations of the hot rolling of U-10Mo coupons encapsulated in low-carbon steel were conducted following two different schedules. Model predictions of the roll-separation force and roll pack thicknesses at different stages of the rolling process were compared with experimental measurements. The study reported here discussed various attributes of the rolled coupons revealed by the model (e.g., waviness and thickness non-uniformity like dog boning). To investigate the influence of the cladding material on these rolling defects, other cases were simulated:  hot rolling with alternative can materials, namely, 304 stainless steel and Zircaloy-2, and bare-rolling.

Hot rolling of thick uranium molybdenum alloys

Science.gov (United States)

DeMint, Amy L.; Gooch, Jack G.

2015-11-17

Disclosed herein are processes for hot rolling billets of uranium that have been alloyed with about ten weight percent molybdenum to produce cold-rollable sheets that are about one hundred mils thick. In certain embodiments, the billets have a thickness of about 7/8 inch or greater. Disclosed processes typically involve a rolling schedule that includes a light rolling pass and at least one medium rolling pass. Processes may also include reheating the rolling stock and using one or more heavy rolling passes, and may include an annealing step.

MicroRNA-608 and microRNA-34a regulate chordoma malignancy by targeting EGFR, Bcl-xL and MET.

Directory of Open Access Journals (Sweden)

Ying Zhang

Full Text Available Chordomas are rare malignant tumors that originate from the notochord remnants and occur in the skull base, spine and sacrum. Due to a very limited understanding of the molecular pathogenesis of chordoma, there are no adjuvant and molecular therapies besides surgical resection and radiation therapy. microRNAs (miRNAs are small noncoding regulatory RNA molecules with critical roles in cancer. The role of miRNAs in chordomas is mostly unknown. We uncover microRNA-608 (miR-608 and microRNA-34a (miR-34a as novel tumor suppressive microRNAs that regulate malignancy in chordoma. We find that miR-608 and miR-34a expressions are downregulated in human chordoma cell lines and primary cells at least partially via alteration of their genes' copy numbers. We identify the commonly deregulated oncogenes EGFR and Bcl-xL as direct targets of miR-608 and the receptor tyrosine kinase MET as direct target of miR-34a. We show that EGFR and MET activations promote chordoma cell proliferation and invasion and that pharmacological inhibition of EGFR and MET inhibits chordoma cell proliferation and survival. We demonstrate that restoration of miR-608 and miR-34a inhibits cell proliferation and invasion and induces apoptosis in chordoma cells. We find that miR-34a inversely correlates with MET expression and miR-608 inversely correlates with EGFR expression in chordoma cells. These findings demonstrate for the first time that miR-608 and miR-34a regulate chordoma malignancy by regulating EGFR, MET and Bcl-xL.

Mutant forms of Escherichia coli protein L25 unable to bind to 5S rRNA are incorporated efficiently into the ribosome in vivo.

Science.gov (United States)

Anikaev, A Y; Korepanov, A P; Korobeinikova, A V; Kljashtorny, V G; Piendl, W; Nikonov, S V; Garber, M B; Gongadze, G M

2014-08-01

5S rRNA-binding ribosomal proteins of the L25 family are an evolutional acquisition of bacteria. Earlier we showed that (i) single replacements in the RNA-binding module of the protein of this family result in destabilization or complete impossibility to form a complex with 5S rRNA in vitro; (ii) ΔL25 ribosomes of Escherichia coli are less efficient in protein synthesis in vivo than the control ribosomes. In the present work, the efficiency of incorporation of the E. coli protein L25 with mutations in the 5S rRNA-binding region into the ribosome in vivo was studied. It was found that the mutations in L25 that abolish its ability to form the complex with free 5S rRNA do not prevent its correct and efficient incorporation into the ribosome. This is supported by the fact that even the presence of a very weakly retained mutant form of the protein in the ribosome has a positive effect on the activity of the translational machinery in vivo. All this suggests the existence of an alternative incorporation pathway for this protein into the ribosome, excluding the preliminary formation of the complex with 5S rRNA. At the same time, the stable L25-5S rRNA contact is important for the retention of the protein within the ribosome, and the conservative amino acid residues of the RNA-binding module play a key role in this.

Ship Roll Damping Control

DEFF Research Database (Denmark)

Perez, Tristan; Blanke, Mogens

2012-01-01

limitations and large variations of the spectral characteristics of wave-induced roll motion. This tutorial paper presents an account of the development of various ship roll motion control systems together with the challenges associated with their design. It discusses the assessment of performance...

Improved Dutch Roll Approximation for Hypersonic Vehicle

Directory of Open Access Journals (Sweden)

Liang-Liang Yin

2014-06-01

Full Text Available An improved dutch roll approximation for hypersonic vehicle is presented. From the new approximations, the dutch roll frequency is shown to be a function of the stability axis yaw stability and the dutch roll damping is mainly effected by the roll damping ratio. In additional, an important parameter called roll-to-yaw ratio is obtained to describe the dutch roll mode. Solution shows that large-roll-to-yaw ratio is the generate character of hypersonic vehicle, which results the large error for the practical approximation. Predictions from the literal approximations derived in this paper are compared with actual numerical values for s example hypersonic vehicle, results show the approximations work well and the error is below 10 %.

Efficient Circulation of Railway Rolling Stock

NARCIS (Netherlands)

Alfieri, A.; Groot, R.; Kroon, L.G.; Schrijver, A.

2006-01-01

Railway rolling stock (locomotives, carriages, and train units) is one of the most significant cost sources for operatorsof passenger trains, both public and private. Rolling stock costsare due to material acquisition, power supply, and material maintenance. The efficient circulation of rolling

Small-Molecule Organic Photovoltaic Modules Fabricated via Halogen-Free Solvent System with Roll-to-Roll Compatible Scalable Printing Method.

Science.gov (United States)

Heo, Youn-Jung; Jung, Yen-Sook; Hwang, Kyeongil; Kim, Jueng-Eun; Yeo, Jun-Seok; Lee, Sehyun; Jeon, Ye-Jin; Lee, Donmin; Kim, Dong-Yu

2017-11-15

For the first time, the photovoltaic modules composed of small molecule were successfully fabricated by using roll-to-roll compatible printing techniques. In this study, blend films of small molecules, BTR and PC 71 BM were slot-die coated using a halogen-free solvent system. As a result, high efficiencies of 7.46% and 6.56% were achieved from time-consuming solvent vapor annealing (SVA) treatment and roll-to-roll compatible solvent additive approaches, respectively. After successful verification of our roll-to-roll compatible method on small-area devices, we further fabricated large-area photovoltaic modules with a total active area of 10 cm 2 , achieving a power conversion efficiency (PCE) of 4.83%. This demonstration of large-area photovoltaic modules through roll-to-roll compatible printing methods, even based on a halogen-free solvent, suggests the great potential for the industrial-scale production of organic solar cells (OSCs).

Die Rolle der microRNA-15b in der Pathogenese des experimentellen allergischen Asthma bronchiale

OpenAIRE

Müllers, Charlotte; Renz, Harald (Prof. Dr. med.)

2016-01-01

Das allergische Asthma bronchiale zeichnet sich durch eine von T-Helferzellen gesteuerte, komplexe und bis heute nicht vollständig verstandene chronische Entzündungsreaktion aus. Auch wenn im Rahmen des Krankheitsbildes zunehmend von einer Heterogenität und Plastizität der T-Helferzellen insgesamt ausgegangen wird, so nimmt die Gruppe der TH2-Zellen im Speziellen weiterhin eine zentrale Rolle in der Pathogenese der Erkrankung ein....

Self-(Un)rolling Biopolymer Microstructures: Rings, Tubules, and Helical Tubules from the Same Material.

Science.gov (United States)

Ye, Chunhong; Nikolov, Svetoslav V; Calabrese, Rossella; Dindar, Amir; Alexeev, Alexander; Kippelen, Bernard; Kaplan, David L; Tsukruk, Vladimir V

2015-07-13

We have demonstrated the facile formation of reversible and fast self-rolling biopolymer microstructures from sandwiched active-passive, silk-on-silk materials. Both experimental and modeling results confirmed that the shape of individual sheets effectively controls biaxial stresses within these sheets, which can self-roll into distinct 3D structures including microscopic rings, tubules, and helical tubules. This is a unique example of tailoring self-rolled 3D geometries through shape design without changing the inner morphology of active bimorph biomaterials. In contrast to traditional organic-soluble synthetic materials, we utilized a biocompatible and biodegradable biopolymer that underwent a facile aqueous layer-by-layer (LbL) assembly process for the fabrication of 2D films. The resulting films can undergo reversible pH-triggered rolling/unrolling, with a variety of 3D structures forming from biopolymer structures that have identical morphology and composition. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Multi-level approach for parametric roll analysis

Science.gov (United States)

Kim, Taeyoung; Kim, Yonghwan

2011-03-01

The present study considers multi-level approach for the analysis of parametric roll phenomena. Three kinds of computation method, GM variation, impulse response function (IRF), and Rankine panel method, are applied for the multi-level approach. IRF and Rankine panel method are based on the weakly nonlinear formulation which includes nonlinear Froude- Krylov and restoring forces. In the computation result of parametric roll occurrence test in regular waves, IRF and Rankine panel method show similar tendency. Although the GM variation approach predicts the occurrence of parametric roll at twice roll natural frequency, its frequency criteria shows a little difference. Nonlinear roll motion in bichromatic wave is also considered in this study. To prove the unstable roll motion in bichromatic waves, theoretical and numerical approaches are applied. The occurrence of parametric roll is theoretically examined by introducing the quasi-periodic Mathieu equation. Instability criteria are well predicted from stability analysis in theoretical approach. From the Fourier analysis, it has been verified that difference-frequency effects create the unstable roll motion. The occurrence of unstable roll motion in bichromatic wave is also observed in the experiment.

Preorganization of Nanostructured Inks for Roll-to-Roll-Coated Polymer Solar Cells

DEFF Research Database (Denmark)

Krebs, Frederik C; Senkovskyy, Volodymyr; Kiriy, Anton

2010-01-01

, a preorganized ink was obtained that was used to make polymer solar cell modules in a full roll-to-roll coating and printing process operating in ambient air. The polymer solar cells were thus prepared by a mixture of slot die and flat-bed screen printing. Various polymer solar cell modules were prepared ranging...

Self-Aligned Metal Electrodes in Fully Roll-to-Roll Processed Organic Transistors

Directory of Open Access Journals (Sweden)

Marja Vilkman

2016-01-01

Full Text Available We demonstrate the production of organic bottom gate transistors with self-aligned electrodes, using only continuous roll-to-roll (R2R techniques. The self-alignment allows accurate <5 µm layer-to-layer registration, which is usually a challenge in high-speed R2R environments as the standard registration methods are limited to the millimeter range—or, at best, to tens of µm if online cameras and automatic web control are utilized. The improved registration enables minimizing the overlap between the source/drain electrodes and the gate electrode, which is essential for minimizing the parasitic capacitance. The complete process is a combination of several techniques, including evaporation, reverse gravure, flexography, lift-off, UV exposure and development methods—all transferred to a continuous R2R pilot line. Altogether, approximately 80 meters of devices consisting of thousands of transistors were manufactured in a roll-to-roll fashion. Finally, a cost analysis is presented in order to ascertain the main costs and to predict whether the process would be feasible for the industrial production of organic transistors.

The Critical Choice of PEDOT: PSS Additives for Long Term Stability of Roll‐to‐Roll Processed OPVs

DEFF Research Database (Denmark)

Roth, Bérenger; Benatto, Gisele Alves dos Reis; Corazza, Michael

2015-01-01

The impact of additives mixed with poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) on the stability of organic photovoltaic modules is investigated for fully ambient roll-to-roll (R2R) processed indium tin oxide free modules. Four different PEDOT:PSS inks from two different...... on organic photovoltaic stability (ISOS). For the harsh indoor test (ISOS-L-3) only a slight difference in stability is observed between the different modules. During both ISOS-L-3 and ISOS-D-3 one new failure mode is observed as a result of tiny air inclusions in the barrier foil and a R2R method...

Roll-to-Roll fabrication of large area functional organic materials

DEFF Research Database (Denmark)

Søndergaard, Roar R.; Hösel, Markus; Krebs, Frederik C

2013-01-01

With the prospect of extremely fast manufacture of very low cost devices, organic electronics prepared by thin film processing techniques that are compatible with roll-to-roll (R2R) methods are presently receiving an increasing interest. Several technologies using organic thin films...... research fields such as organic photovoltaics, organic thin film transistors, light-emitting diodes, polymer electrolyte membrane fuel cells, and electrochromic devices. © 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 000: 000–000, 2012...

Perovskite solar cells for roll-to-roll fabrication

Directory of Open Access Journals (Sweden)

Uddin Ashraf

2017-01-01

Full Text Available Perovskite solar cell (PSCs is considered as the game changer in emerging photovoltaics technology. The highest certified efficiency is 22% with high temperature processed (∼500 °C TiO2 based electron transport layer (ETL. High temperature process is a rudimentary hindrance towards roll-to-roll processing of PSCs on flexible substrates. Low temperature solution process (<150 °C ZnO based ETL is one of the most promising candidate for large scale roll-to-roll fabrication of cells as it has nearly identical electron affinity (4.2 eV of TiO2. The mixed organic perovskite (MA0.6FA0.4PbI3 devices with Al doped ZnO (AZO ETL demonstrate average cell efficiency over 16%, which is the highest ever reported efficiency for this device configuration. The energy level alignment and related interfacial charge transport dynamics at the interface of ZnO and perovskite films and the adjacent charge transport layers are investigated. Significantly improved device stability, hysteresis free device photocurrent have been observed in MA0.6FA0.4PbI3 cells. A systematic electrochemical impedance spectroscopy, frequency dependent capacitance spectra, surface morphology and topography characterization have been conducted to understand the role of interfacial electronic properties between perovskite and neighbouring layers in perovskite device. A standardized degradation study, interfacial electronic property and capacitive spectra analysis of aged device, have been measured to understand the enhanced device stability in mixed MA0.6FA0.4PbI3 cells. Slow perovskite material decomposition rate and augmented device lifetime with AZO based devices have been found to be correlated with the more hydrophobic and acidic nature of AZO surface compared to pristine ZnO film.

TBT recommends : Estonian Music Awards. First Rock'n'Roll Festival. Fantazy

Index Scriptorium Estoniae

2005-01-01

Muusikaauhindade jagamise üritusest 2. märtsil Tallinna klubis Parlament. 26. veebr. rahvusvahelisest Rock'n'Roll Festivalist Lätis (esinevad eesti ansambel Chick'n Band, ansamblid Boogie D, Wildfire Willie & the Ramblers ning läti pianist Pete Anderson). Vodevillide õhtust 25. veebr. klubis "New York" Vilniuses

Roll-to-roll coated PBI membranes for high temperature PEM fuel cells

DEFF Research Database (Denmark)

Steenberg, Thomas; Hjuler, Hans Aage; Terkelsen, Carina

2012-01-01

We employed roll-to-roll coating in the preparation of 40 μm thick poly[2,2′(m-phenylene)-5,5′bibenzimidazole] (PBI) films for fuel cells using both knife-coating (KC) and slot-die (SD) coating. The films were coated directly from a 9% (w/w) solution of PBI in dimethylacetamide onto a sacrificial...

Troll, a Language for specifying Dice-rolls

DEFF Research Database (Denmark)

Mogensen, Torben Ægidius

2009-01-01

Dice are used in many games, and often in fairly complex ways that make it difficult to unambiguously describe the dice-roll mechanism in plain language. Many role-playing games, such as Dungeons & Dragons, use a formalised notation for some instances of dice-rolls. This notation, once explained...... natural language to describe rolls. Even Dungeons & Dragons use formal notation only for some of the dice-roll methods used in the game. Hence, a more complete notation is in this paper proposed, and a tool for pseudo-random rolls and (nearly) exact probability calculations is described. The notation...... is called "Troll", combining the initial of the Danish word for dice ("terninger") with the English word "roll". It is a development of the language Roll described in an earlier paper. The present paper describes the most important features of Troll and its implementation....

Effects of alloying elements on sticking occurring during hot rolling of ferritic stainless steels

International Nuclear Information System (INIS)

Ha, Dae Jin; Kim, Yong Jin; Lee, Yong Deuk; Lee, Sung Hak; Lee, Jong Seog

2008-01-01

In this study, effects of alloying elements on the sticking occurring during hot rolling of five kinds of ferritic STS430J1L stainless steels were investigated by analyzing high-temperature hardness and oxidation behavior of the rolled steels. Hot-rolling simulation tests were conducted by a high-temperature wear tester which could simulate actual hot rolling. The simulation test results revealed that the sticking process proceeded with three stages, i.e., nucleation, growth, and saturation. Since the hardness continuously decreased as the test temperature increased, whereas the formation of Fe-Cr oxides in the rolled steel surface region increased, the sticking of five stainless steels was evaluated by considering both the high-temperature hardness and oxidation effects. The addition of Zr, Cu, or Si had a beneficial effect on the sticking resistance, while the Ni addition did not show any difference in the sticking. Particularly in the case of the Si addition, Si oxides formed first in the initial stage of high-temperature oxidation, worked as initiation sites for Fe-Cr oxides, accelerated the formation of Fe-Cr oxides, and thus raised the sticking resistance by about 10 times in comparison with the steel without Si content

Autonomous Supervision and Control of Parametric Roll Resonance

DEFF Research Database (Denmark)

Galeazzi, Roberto

therefore two objectives. The first is to develop methods for detection of the inception of parametric roll resonance. The second is to develop control strategies to stabilize the motion after parametric roll has started. Stabilisation of parametric roll resonance points to two possible courses of action...... strategies are then combined to stabilise parametric roll resonance within few roll cycles. Limitations on the maximum stabilisable roll angle are analysed and linked to the ii slew rate saturation and hydrodynamic stall characteristics of the fin stabilisers. The study on maximum stabilisable roll angle...... leads to the requirements for early detection. Two novel detectors are proposed, which work within a shorttime prediction horizon, and issue early warnings of parametric roll inception within few roll cycles from its onset. The main idea behind these detection schemes is that of exploiting the link...

New perspectives on constant-roll inflation

Science.gov (United States)

Cicciarella, Francesco; Mabillard, Joel; Pieroni, Mauro

2018-01-01

We study constant-roll inflation using the β-function formalism. We show that the constant rate of the inflaton roll is translated into a first order differential equation for the β-function which can be solved easily. The solutions to this equation correspond to the usual constant-roll models. We then construct, by perturbing these exact solutions, more general classes of models that satisfy the constant-roll equation asymptotically. In the case of an asymptotic power law solution, these corrections naturally provide an end to the inflationary phase. Interestingly, while from a theoretical point of view (in particular in terms of the holographic interpretation) these models are intrinsically different from standard slow-roll inflation, they may have phenomenological predictions in good agreement with present cosmological data.

Itaalia ja Belgia samuti kahtluse all. Reitingud ohus / Sirje Rank

Index Scriptorium Estoniae

Rank, Sirje, 1966-

2011-01-01

Agentuur S&P vähendas Itaalia reitinguväljavaadet, seda tingis Itaalia suur võlakoorem. Ka Belgia reitinguväljavaadet vähendati suure võlakoorma tõttu. Hispaania puhul on usaldus löönud kõikuma seoses peaministri Jose Zapatero partei kaotusega valimistel - kardetakse, et valitsus võib enne üldvalimisi vajalikke reforme kokku tõmmata

Maintenance of the marginal-zone B cell compartment specifically requires the RNA-binding protein ZFP36L1.

Science.gov (United States)

Newman, Rebecca; Ahlfors, Helena; Saveliev, Alexander; Galloway, Alison; Hodson, Daniel J; Williams, Robert; Besra, Gurdyal S; Cook, Charlotte N; Cunningham, Adam F; Bell, Sarah E; Turner, Martin

2017-06-01

RNA-binding proteins of the ZFP36 family are best known for inhibiting the expression of cytokines through binding to AU-rich elements in the 3' untranslated region and promoting mRNA decay. Here we identified an indispensable role for ZFP36L1 as the regulator of a post-transcriptional hub that determined the identity of marginal-zone B cells by promoting their proper localization and survival. ZFP36L1 controlled a gene-expression program related to signaling, cell adhesion and locomotion; it achieved this in part by limiting expression of the transcription factors KLF2 and IRF8, which are known to enforce the follicular B cell phenotype. These mechanisms emphasize the importance of integrating transcriptional and post-transcriptional processes by RNA-binding proteins for maintaining cellular identity among closely related cell types.

High-speed DNA-based rolling motors powered by RNase H

Science.gov (United States)

Yehl, Kevin; Mugler, Andrew; Vivek, Skanda; Liu, Yang; Zhang, Yun; Fan, Mengzhen; Weeks, Eric R.

2016-01-01

DNA-based machines that walk by converting chemical energy into controlled motion could be of use in applications such as next generation sensors, drug delivery platforms, and biological computing. Despite their exquisite programmability, DNA-based walkers are, however, challenging to work with due to their low fidelity and slow rates (~1 nm/min). Here, we report DNA-based machines that roll rather than walk, and consequently have a maximum speed and processivity that is three-orders of magnitude greater than conventional DNA motors. The motors are made from DNA-coated spherical particles that hybridise to a surface modified with complementary RNA; motion is achieved through the addition of RNase H, which selectively hydrolyses hybridised RNA. Spherical motors move in a self-avoiding manner, whereas anisotropic particles, such as dimerised particles or rod-shaped particles travel linearly without a track or external force. Finally, we demonstrate detection of single nucleotide polymorphism by measuring particle displacement using a smartphone camera. PMID:26619152

Calculation and experimental technique of determination of rolling procedure for cold-rolling tube mills

International Nuclear Information System (INIS)

Igoshin, V.F.; Aleshin, V.A.; Khoroshikh, Yu.G.; Bogatov, A.A.; Mizhiritskij, O.I.

1983-01-01

Calculation and experimental technique of determination of tube cold rolling procedure has been developed. Rolling procedure based on the usage of regression equation epsilon=1.24 psi, where psi is the relative reduction of area, delta-permissible reduction during rolling, has been tested on 08Kh18N10T steel. The effect of tube geometry, tool calibration parameters, lubrication conditions etc. on metal deformability in taking into account experimentally. The use of the technique proposed has allowed to shorten the time of mastering of the production of tubes from different steels

Enhancing roll stability of heavy vehicle by LQR active anti-roll bar control using electronic servo-valve hydraulic actuators

Science.gov (United States)

Vu, Van Tan; Sename, Olivier; Dugard, Luc; Gaspar, Peter

2017-09-01

Rollover of heavy vehicle is an important road safety problem world-wide. Although rollovers are relatively rare events, they are usually deadly accidents when they occur. The roll stability loss is the main cause of rollover accidents in which heavy vehicles are involved. In order to improve the roll stability, most of modern heavy vehicles are equipped with passive anti-roll bars to reduce roll motion during cornering or riding on uneven roads. However these may be not sufficient to overcome critical situations. This paper introduces the active anti-roll bars made of four electronic servo-valve hydraulic actuators, which are modelled and integrated in a yaw-roll model of a single unit heavy vehicle. The control signal is the current entering the electronic servo-valve and the output is the force generated by the hydraulic actuator. The active control design is achieved solving a linear optimal control problem based on the linear quadratic regulator (LQR) approach. A comparison of several LQR controllers is provided to allow for tackling the considered multi-objective problems. Simulation results in frequency and time domains show that the use of two active anti-roll bars (front and rear axles) drastically improves the roll stability of the single unit heavy vehicle compared with the passive anti-roll bar.

Characteristics and Prediction of RNA Structure

Directory of Open Access Journals (Sweden)

Hengwu Li

2014-01-01

Full Text Available RNA secondary structures with pseudoknots are often predicted by minimizing free energy, which is NP-hard. Most RNAs fold during transcription from DNA into RNA through a hierarchical pathway wherein secondary structures form prior to tertiary structures. Real RNA secondary structures often have local instead of global optimization because of kinetic reasons. The performance of RNA structure prediction may be improved by considering dynamic and hierarchical folding mechanisms. This study is a novel report on RNA folding that accords with the golden mean characteristic based on the statistical analysis of the real RNA secondary structures of all 480 sequences from RNA STRAND, which are validated by NMR or X-ray. The length ratios of domains in these sequences are approximately 0.382L, 0.5L, 0.618L, and L, where L is the sequence length. These points are just the important golden sections of sequence. With this characteristic, an algorithm is designed to predict RNA hierarchical structures and simulate RNA folding by dynamically folding RNA structures according to the above golden section points. The sensitivity and number of predicted pseudoknots of our algorithm are better than those of the Mfold, HotKnots, McQfold, ProbKnot, and Lhw-Zhu algorithms. Experimental results reflect the folding rules of RNA from a new angle that is close to natural folding.

Scalable, ambient atmosphere roll-to-roll manufacture of encapsulated large area, flexible organic tandem solar cell modules

DEFF Research Database (Denmark)

Andersen, Thomas Rieks; Dam, Henrik Friis; Hösel, Markus

2014-01-01

the manufacture of completely functional devices in exceptionally high yields. Critical to the ink and process development is a carefully chosen technology transfer to industry method where first a roll coater is employed enabling contactless stack build up, followed by a small roll-to-roll coater fitted to an X...

Stress Corrosion Cracking Susceptibility of 304L Substrate and 308L Weld Metal Exposed to a Salt Spray

Directory of Open Access Journals (Sweden)

Chia-Hao Hsu

2017-02-01

Full Text Available 304 stainless steels (SS were considered as the materials for a dry storage canister. In this study, ER (Electrode Rod 308L was utilized as the filler metal for the groove and overlay welds of a 304L stainless steel substrate, which was prepared via a gas tungsten arc-welding process in multiple passes. The electron backscatter diffraction (EBSD map was used to identify the inherent microstructures in distinct specimens. U-bend and weight-loss tests were conducted by testing the 304L substrates and welds in a salt spray containing 5 wt % NaCl at 80 °C to evaluate their susceptibility to stress corrosion cracking (SCC. Generally, the weight loss of the ER 308L deposit was higher than that of the 304L substrate in a salt spray in the same sample-prepared condition. The dissolution of the skeletal structure in the fusion zone (FZ was responsible for a greater weight loss of the 308L deposit, especially for the cold-rolled and sensitized specimen. Cold rolling was detrimental and sensitization after cold rolling was very harmful to the SCC resistance of the 304L substrate and 308L deposit. Overall, the SCC susceptibility of each specimen was correlated with its weight loss in each group.

Systematics of constant roll inflation

Science.gov (United States)

Anguelova, Lilia; Suranyi, Peter; Wijewardhana, L. C. R.

2018-02-01

We study constant roll inflation systematically. This is a regime, in which the slow roll approximation can be violated. It has long been thought that this approximation is necessary for agreement with observations. However, recently it was understood that there can be inflationary models with a constant, and not necessarily small, rate of roll that are both stable and compatible with the observational constraint ns ≈ 1. We investigate systematically the condition for such a constant-roll regime. In the process, we find a whole new class of inflationary models, in addition to the known solutions. We show that the new models are stable under scalar perturbations. Finally, we find a part of their parameter space, in which they produce a nearly scale-invariant scalar power spectrum, as needed for observational viability.

Enhanced production of L-sorbose from D-sorbitol by improving the mRNA abundance of sorbitol dehydrogenase in Gluconobacter oxydans WSH-003.

Science.gov (United States)

Xu, Sha; Wang, Xiaobei; Du, Guocheng; Zhou, Jingwen; Chen, Jian

2014-10-18

Production of L-sorbose from D-sorbitol by Gluconobacter oxydans is the first step to produce L-ascorbic acid on industrial scale. The sldhAB gene, which encodes the sorbitol dehydrogenase (SLDH), was overexpressed in an industrial strain G. oxydans WSH-003 with a strong promoter, P tufB . To enhance the mRNA abundance, a series of artificial poly(A/T) tails were added to the 3'-terminal of sldhAB gene. Besides, their role in sldhAB overexpression and their subsequent effects on L-sorbose production were investigated. The mRNA abundance of the sldhAB gene could be enhanced in G. oxydans by suitable poly(A/T) tails. By self-overexpressing the sldhAB gene in G. oxydans WSH-003 with an optimal poly(A/T) tail under the constitutive promoter P tufB , the titer and the productivity of L-sorbose were enhanced by 36.3% and 25.0%, respectively, in a 1-L fermenter. Immobilization of G. oxydans-sldhAB6 cells further improved the L-sorbose titer by 33.7% after 20 days of semi-continuous fed-batch fermentation. The artificial poly(A/T) tails could significantly enhance the mRNA abundance of the sldhAB. Immobilized G. oxydans-sldhAB6 cells could further enlarge the positive effect caused by enhanced mRNA abundance of the sldhAB.

Constant-roll tachyon inflation and observational constraints

Science.gov (United States)

Gao, Qing; Gong, Yungui; Fei, Qin

2018-05-01

For the constant-roll tachyon inflation, we derive the analytical expressions for the scalar and tensor power spectra, the scalar and tensor spectral tilts and the tensor to scalar ratio to the first order of epsilon1 by using the method of Bessel function approximation. The derived ns-r results are compared with the observations, we find that only the constant-roll inflation with ηH being a constant is consistent with the observations and observations constrain the constant-roll inflation to be slow-roll inflation. The tachyon potential is also reconstructed for the constant-roll inflation which is consistent with the observations.

Anisotropic constant-roll inflation

Energy Technology Data Exchange (ETDEWEB)

Ito, Asuka; Soda, Jiro [Kobe University, Department of Physics, Kobe (Japan)

2018-01-15

We study constant-roll inflation in the presence of a gauge field coupled to an inflaton. By imposing the constant anisotropy condition, we find new exact anisotropic constant-roll inflationary solutions which include anisotropic power-law inflation as a special case. We also numerically show that the new anisotropic solutions are attractors in the phase space. (orig.)

Cosmology with rolling tachyon

Indian Academy of Sciences (India)

Email: sami@iucaa.ernet.in. Abstract. We examine the possibility of rolling tachyon to play the dual role of inflaton at early epochs and dark matter at late times. We argue that enough inflation can be generated with the rolling tachyon either by invoking the large number of branes or brane world assisted inflation. However ...

METHOD OF HOT ROLLING URANIUM METAL

Science.gov (United States)

Kaufmann, A.R.

1959-03-10

A method is given for quickly and efficiently hot rolling uranium metal in the upper part of the alpha phase temperature region to obtain sound bars and sheets possessing a good surface finish. The uranium metal billet is heated to a temperature in the range of 1000 deg F to 1220 deg F by immersion iii a molten lead bath. The heated billet is then passed through the rolls. The temperature is restored to the desired range between successive passes through the rolls, and the rolls are turned down approximately 0.050 inch between successive passes.

Microstructure formation via roll-to-roll UV embossing using a flexible mould made from a laminated polymer–copper film

International Nuclear Information System (INIS)

Zhong, Z W; Shan, X C

2012-01-01

Roll-to-roll large format UV embossing processes aim to revolutionize the manufacturing of functional films, with the ability to process a large area at one time, resulting in high throughput and cost reduction. In this paper, we present the experimental results obtained during the process development for roll-to-roll large format UV embossing. Flexible moulds were fabricated from a hybrid film substrate made of a liquid crystal polymer with clad copper foils laminated on both sides of it. The effective pattern area of the fabricated flexible mould was 400 mm × 300 mm with a minimal feature size of 50 µm. The results show that the roll-to-roll embossing processes are capable of producing micro-scale structures and functional devices over a large area at one time. Large-area roll-to-roll embossing was demonstrated by using the hybrid flexible mould, and micro-features and structures such as micro-channels and dot arrays were replicated on thermoplastic substrates. In addition to its ease and low cost in fabrication, the hybrid flexible moulds demonstrated to have acceptable fidelity and durability. The hybrid flexible mould is a novel solution for large-area embossing. (paper)

75 FR 32160 - Certain Hot-Rolled Flat-Rolled Carbon-Quality Steel Products from Brazil: Extension of Time Limit...

Science.gov (United States)

2010-06-07

...-Rolled Carbon-Quality Steel Products from Brazil: Extension of Time Limit for Preliminary Results of...-quality steel products from Brazil. See Agreement Suspending the Countervailing Duty Investigation on Hot... duty order on certain hot-rolled flat-rolled carbon-quality steel products from Brazil. See Initiation...

Microstructural evolution in warm-rolled and cold-rolled strip cast 6.5 wt% Si steel thin sheets and its influence on magnetic properties

Energy Technology Data Exchange (ETDEWEB)

Wang, Xianglong, E-mail: 215454278@qq.com; Liu, Zhenyu, E-mail: zyliu@mail.neu.edu.cn; Li, Haoze; Wang, Guodong

2017-07-01

Highlights: • The experimental materials used in the study are based on strip casting. • Magnetic properties between warm rolled and cold rolled sheets are investigated. • Cold rolled 6.5% Si sheet has better magnetic properties than warm rolled sheet. • The γ and λ-fiber recrystallization textures can be optimized after cold rolling. • Cold rolling should be more suitable for fabricating 6.5% Si steel thin sheets. - Abstract: 6.5 wt% Si steel thin sheets were usually fabricated by warm rolling. In our previous work, 6.5 wt% Si steel thin sheets with good magnetic properties had been successfully fabricated by cold rolling based on strip casting. In the present work, the main purposes were to find out the influences of warm rolling and cold rolling on microstructures and magnetic properties of the thin sheets with the thickness of 0.2 mm, and to confirm which rolling method was more suitable for fabricating 6.5 wt% Si steel thin sheets. The results showed that the cold rolled sheet could obtain good surface quality and flatness, while the warm rolled sheet could not. The intensity of γ-fiber rolling texture (<1 1 1>//ND) of cold rolled specimen was weaker than that of the warm rolled specimen, especially for the {1 1 1}<1 1 2> component at surface layer and {1 1 1}<1 1 0> component at center layer. After the same annealing treatment, the cold rolled specimen, which had higher stored energy and weaker intensity of γ-fiber rolling texture, could obtain smaller recrystallization grain size, weaker intensity of γ-fiber recrystallization texture and stronger intensity of λ-fiber recrystallization texture. Therefore, due to the good surface quality, smaller recrystallization grain size and optimum recrystallization texture, the cold rolled specimen possessed improved magnetic properties, and cold rolling should be more suitable for fabricating 6.5 wt% Si steel thin sheets.

Just Roll with It? Rolling Volumes vs. Discrete Issues in Open Access Library and Information Science Journals

Directory of Open Access Journals (Sweden)

Jill Cirasella

2013-08-01

Full Text Available INTRODUCTION Articles in open access (OA journals can be published on a rolling basis, as they become ready, or in complete, discrete issues. This study examines the prevalence of and reasons for rolling volumes vs. discrete issues among scholarly OA library and information science (LIS journals based in the United States. METHODS A survey was distributed to journal editors, asking them about their publication model and their reasons for and satisfaction with that model. RESULTS Of the 21 responding journals, 12 publish in discrete issues, eight publish in rolling volumes, and one publishes in rolling volumes with an occasional special issue. Almost all editors, regardless of model, cited ease of workflow as a justification for their chosen publication model, suggesting that there is no single best workflow for all journals. However, while all rolling-volume editors reported being satisfied with their model, satisfaction was less universal among discrete-issue editors. DISCUSSION The unexpectedly high number of rolling-volume journals suggests that LIS journal editors are making forward-looking choices about publication models even though the topic has not been much addressed in the library literature. Further research is warranted; possibilities include expanding the study’s geographic scope, broadening the study to other disciplines, and investigating publication model trends across the entire scholarly OA universe. CONCLUSION Both because satisfaction is high among editors of rolling-volume journals and because readers and authors appreciate quick publication times, the rolling-volume model will likely become even more prevalent in coming years.

Roll-to-roll compatible organic thin film transistor manufacturing technique by printing, lamination, and laser ablation

International Nuclear Information System (INIS)

Hassinen, Tomi; Ruotsalainen, Teemu; Laakso, Petri; Penttilä, Raimo; Sandberg, Henrik G.O.

2014-01-01

We present roll-to-roll printing compatible techniques for manufacturing organic thin film transistors using two separately processed foils that are laminated together. The introduction of heat-assisted lamination opens up possibilities for material and processing combinations. The lamination of two separately processed substrates together will allow usage of pre-patterned electrodes on both substrates and materials with non-compatible solvents. Also, the surface microstructure is formed differently when laminating dry films together compared to film formation from liquid phase. Demonstrator transistors, inverters and ring oscillators were produced using lamination techniques. Finally, a roll-to-roll compatible lamination concept is proposed where also the source and drain electrodes are patterned by laser ablation. The demonstrator transistors have shown very good lifetime in air, which is contributed partly to the good material combination and partly to the enhanced interface formation in heat-assisted lamination process. - Highlights: • A roll-to-roll compatible lamination technique for printed electronics is proposed. • Laser ablation allows highly defined metal top and bottom electrodes. • Method opens up processing possibilities for incompatible materials and solvents. • Shearing forces may enhance molecular orientation and packing. • An air stable polymer transistor is demonstrated with a lifetime of years

Inflation with a constant rate of roll

International Nuclear Information System (INIS)

Motohashi, Hayato; Starobinsky, Alexei A.; Yokoyama, Jun'ichi

2015-01-01

We consider an inflationary scenario where the rate of inflaton roll defined by ·· φ/H φ-dot remains constant. The rate of roll is small for slow-roll inflation, while a generic rate of roll leads to the interesting case of 'constant-roll' inflation. We find a general exact solution for the inflaton potential required for such inflaton behaviour. In this model, due to non-slow evolution of background, the would-be decaying mode of linear scalar (curvature) perturbations may not be neglected. It can even grow for some values of the model parameter, while the other mode always remains constant. However, this always occurs for unstable solutions which are not attractors for the given potential. The most interesting particular cases of constant-roll inflation remaining viable with the most recent observational data are quadratic hilltop inflation (with cutoff) and natural inflation (with an additional negative cosmological constant). In these cases even-order slow-roll parameters approach non-negligible constants while the odd ones are asymptotically vanishing in the quasi-de Sitter regime

High-throughput roll-to-roll X-ray characterization of polymer solar cell active layers

DEFF Research Database (Denmark)

Böttiger, Arvid P.L.; Jørgensen, Mikkel; Menzel, Andreas

2012-01-01

Synchrotron-based X-rays were used to probe active materials for polymer solar cells on flexible polyester foil. The active material was coated onto a flexible 130 micron thick polyester foil using roll-to-roll differentially pumped slot-die coating and presented variation in composition, thickness...

Origins of Rolling Friction

Science.gov (United States)

Cross, Rod

2017-01-01

When a hard object rolls on a soft surface, or vice versa, rolling friction arises from deformation of the soft object or the soft surface. The friction force can be described in terms of an offset in the normal reaction force or in terms of energy loss arising from the deformation. The origin of the friction force itself is not entirely clear. It…

Towards roll-to-roll manufacturing of polymer photonic devices

Science.gov (United States)

Subbaraman, Harish; Lin, Xiaohui; Ling, Tao; Guo, L. Jay; Chen, Ray T.

2014-03-01

Traditionally, polymer photonic devices are fabricated using clean-room processes such as photolithography, e-beam lithography, reactive ion etching (RIE) and lift-off methods etc, which leads to long fabrication time, low throughput and high cost. We have utilized a novel process for fabricating polymer photonic devices using a combination of imprinting and ink jet printing methods, which provides high throughput on a variety of rigid and flexible substrates with low cost. We discuss the manufacturing challenges that need to be overcome in order to realize true implementation of roll-to-roll manufacturing of flexible polymer photonic systems. Several metrology and instrumentation challenges involved such as availability of particulate-free high quality substrate, development and implementation of high-speed in-line and off-line inspection and diagnostic tools with adaptive control for patterned and unpatterned material films, development of reliable hardware, etc need to be addressed and overcome in order to realize a successful manufacturing process. Due to extreme resolution requirements compared to print media, the burden of software and hardware tools on the throughput also needs to be carefully determined. Moreover, the effect of web wander and variations in web speed need to accurately be determined in the design of the system hardware and software. In this paper, we show the realization of solutions for few challenges, and utilizing these solutions for developing a high-rate R2R dual stage ink-jet printer that can provide alignment accuracy of web speed of 5m/min. The development of a roll-to-roll manufacturing system for polymer photonic systems opens limitless possibilities for the deployment of high performance components in a variety of applications including communication, sensing, medicine, agriculture, energy, lighting etc.

Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18

Science.gov (United States)

Smirnov, Alexandre; Entelis, Nina; Martin, Robert P.; Tarassov, Ivan

2011-01-01

5S rRNA is an essential component of ribosomes of all living organisms, the only known exceptions being mitochondrial ribosomes of fungi, animals, and some protists. An intriguing situation distinguishes mammalian cells: Although the mitochondrial genome contains no 5S rRNA genes, abundant import of the nuclear DNA-encoded 5S rRNA into mitochondria was reported. Neither the detailed mechanism of this pathway nor its rationale was clarified to date. In this study, we describe an elegant molecular conveyor composed of a previously identified human 5S rRNA import factor, rhodanese, and mitochondrial ribosomal protein L18, thanks to which 5S rRNA molecules can be specifically withdrawn from the cytosolic pool and redirected to mitochondria, bypassing the classic nucleolar reimport pathway. Inside mitochondria, the cytosolic 5S rRNA is shown to be associated with mitochondrial ribosomes. PMID:21685364

Rolling block mazes are PSPACE-complete

NARCIS (Netherlands)

Buchin, K.; Buchin, M.

2012-01-01

In a rolling block maze, one or more blocks lie on a rectangular board with square cells. In most mazes, the blocks have size k × m × n where k, m, n are integers that determine the size of the block in terms of units of the size of the board cells. The task of a rolling block maze is to roll a

Maintenance Appointments in Railway Rolling Stock Rescheduling

NARCIS (Netherlands)

J.C. Wagenaar (Joris); L.G. Kroon (Leo); M.E. Schmidt (Marie)

2016-01-01

textabstractThis paper addresses the Rolling Stock Rescheduling Problem (RSRP), while taking maintenance appointments into account. After a disruption, the rolling stock of the disrupted passenger trains has to be rescheduled in order to restore a feasible rolling stock circulation. Usually, a

Roll-to-roll DBD plasma pretreated polyethylene web for enhancement of Al coating adhesion and barrier property

Energy Technology Data Exchange (ETDEWEB)

Zhang, Haibao; Li, Hua; Fang, Ming; Wang, Zhengduo; Sang, Lijun; Yang, Lizhen; Chen, Qiang, E-mail: lppmchenqiang@hotmail.com

2016-12-01

Graphical abstract: The images of Al coating adhesion testes for (a) untreated and (b) roll-to-roll DBD plasma treated PE. - Highlights: • Over three-months ageing a high surface energy was still existed in roll-to-roll DBD plasma-treated PE surface. • The adhesion and barrier property of Al-coated PE web were greatly improved. • The mechanism of plasma grafting to improve the properties of Al-coated PE web was found. - Abstract: In this paper the roll-to-roll atmospheric dielectric barrier discharge (DBD) was used to pre-treat polyethylene (PE) web surface before the conventional thermal evaporation aluminum (Al) was performed as a barrier layer. We emphasized the plasma environment effect based on the inlet three kinds of reactive monomers. The cross hatch test was employed to assess the Al coating adhesion; and the oxygen transmission rate (OTR) was used to evaluate gas barrier property. The results showed that after roll-to-roll DBD plasma treatment all Al coatings adhered strongly on PE films and were free from pinhole defects with mirror morphology. The OTR was reduced from 2673 cm{sup 3}/m{sup 2} day for Al-coated original PE to 138 cm{sup 3}/m{sup 2} day for Al-coated allyamine (C{sub 3}H{sub 7}N) modified PE. To well understand the mechanism the chemical compositions of the untreated and DBD plasma pretreated PE films were analyzed by X-ray photoelectron spectroscopy (XPS). The surface topography was characterized by atomic force microscopy (AFM). For the property of surface energy the water contact angle measurement was also carried out in the DBD plasma treated samples with deionized water.

Roll-to-roll DBD plasma pretreated polyethylene web for enhancement of Al coating adhesion and barrier property

International Nuclear Information System (INIS)

Zhang, Haibao; Li, Hua; Fang, Ming; Wang, Zhengduo; Sang, Lijun; Yang, Lizhen; Chen, Qiang

2016-01-01

Graphical abstract: The images of Al coating adhesion testes for (a) untreated and (b) roll-to-roll DBD plasma treated PE. - Highlights: • Over three-months ageing a high surface energy was still existed in roll-to-roll DBD plasma-treated PE surface. • The adhesion and barrier property of Al-coated PE web were greatly improved. • The mechanism of plasma grafting to improve the properties of Al-coated PE web was found. - Abstract: In this paper the roll-to-roll atmospheric dielectric barrier discharge (DBD) was used to pre-treat polyethylene (PE) web surface before the conventional thermal evaporation aluminum (Al) was performed as a barrier layer. We emphasized the plasma environment effect based on the inlet three kinds of reactive monomers. The cross hatch test was employed to assess the Al coating adhesion; and the oxygen transmission rate (OTR) was used to evaluate gas barrier property. The results showed that after roll-to-roll DBD plasma treatment all Al coatings adhered strongly on PE films and were free from pinhole defects with mirror morphology. The OTR was reduced from 2673 cm 3 /m 2 day for Al-coated original PE to 138 cm 3 /m 2 day for Al-coated allyamine (C 3 H 7 N) modified PE. To well understand the mechanism the chemical compositions of the untreated and DBD plasma pretreated PE films were analyzed by X-ray photoelectron spectroscopy (XPS). The surface topography was characterized by atomic force microscopy (AFM). For the property of surface energy the water contact angle measurement was also carried out in the DBD plasma treated samples with deionized water.

Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA

DEFF Research Database (Denmark)

Lubas, Michal Szymon; Damgaard, Christian Kroun; Tomecki, Rafal

2013-01-01

Turnover of mRNA in the cytoplasm of human cells is thought to be redundantly conducted by the monomeric 5'-3' exoribonuclease hXRN1 and the 3'-5' exoribonucleolytic RNA exosome complex. However, in addition to the exosome-associated 3'-5' exonucleases hDIS3 and hDIS3L, the human genome encodes...

Design and analysis of roll cage

Science.gov (United States)

Angadi, Gurusangappa; Chetan, S.

2018-04-01

Wildlife fire fighting vehicles are used to extinguish fires in forests, in this process vehicles face falling objects like rocks, tree branches and other objects. Also due to uneven conditions of the terrain like cliff edges, uneven surfaces etc. makes the vehicle to roll over and these can cause injuries to both the driver and the operator. Roll over of a vehicle is a common incident which makes fatal injuries to the operator and also stands next to the crash accidents. In order to reduce the injury level and continuous roll over of the vehicle it is necessary to equip suitable roll cage according to standards of vehicle. In this present work roll cage for pump operator in wildfire fighting vehicle is designed and analysis is carried out in computer simulated environment when seating position of operator seated outside of the cabin. According to NFPA 1906 standards wildlife fire apparatus, Design and Test procedures that are carried out in Hyperworks maintaining SAE J1194.1983 standards. G load case, roof crush analysis and pendulum impact analysis tests are carried out on roll cage to ensure the saftey of design. These load cases are considerd to satisfy the situation faced in forest terrain. In these test procedures roll cage is analysed for stresses and deformation in various load cases. After recording results these are compared with standards mentioned in SAE J1194.1983.

Ribosomal protein L5 has a highly twisted concave surface and flexible arms responsible for rRNA binding.

Science.gov (United States)

Nakashima, T; Yao, M; Kawamura, S; Iwasaki, K; Kimura, M; Tanaka, I

2001-05-01

Ribosomal protein L5 is a 5S rRNA binding protein in the large subunit and plays an essential role in the promotion of a particular conformation of 5S rRNA. The crystal structure of the ribosomal protein L5 from Bacillus stearothermophilus has been determined at 1.8 A resolution. The molecule consists of a five-stranded antiparallel beta-sheet and four alpha-helices, which fold in a way that is topologically similar to the ribonucleoprotein (RNP) domain. The molecular shape and electrostatic representation suggest that the concave surface and loop regions are involved in 5S rRNA binding. To identify amino acid residues responsible for 5S rRNA binding, we made use of Ala-scanning mutagenesis of evolutionarily conserved amino acids occurring in the beta-strands and loop regions. The mutations of Asn37 at the beta1-strand and Gln63 at the loop between helix 2 and beta3-strand as well as that of Phe77 at the tip of the loop structure between the beta2- and beta3-strands caused a significant reduction in 5S rRNA binding. In addition, the mutations of Thr90 on the beta3-strand and Ile141 and Asp144 at the loop between beta4- and beta5-strands moderately reduced the 5S rRNA-binding affinity. Comparison of these results with the more recently analyzed structure of the 50S subunit from Haloarcula marismortui suggests that there are significant differences in the structure at N- and C-terminal regions and probably in the 5S rRNA binding.

Hot Roll Bonding of Aluminum to Twin-Roll Cast (TRC) Magnesium and Its Subsequent Deformation Behavior

Science.gov (United States)

Saleh, H.; Schmidtchen, M.; Kawalla, R.

2018-02-01

In an experiment in which twin-roll cast AZ31 magnesium alloy and commercial purity aluminum (AA 1050) sheets were bonded by hot rolling as Al/Mg/Al laminate composites, it was found that increasing the preheating temperatures up to 400 °C enhances the bonding strength of composites. Further increases in the preheating temperatures accelerate the magnesium oxide growth and thus reduce the bonding strength. The influence of the reduction ratio on the bonding properties was also studied, whereby it was observed that increasing the rolling reduction led to an increase in the bonding strength. The experimental results show that the optimum bonding strength can be obtained at rolling temperatures of 375-400 °C with a 50-60% reduction in thickness. On the other hand, the subsequent deformation behavior of composite was assessed using plane strain compression and deep drawing tests. We demonstrate that the composites produced using the optimum roll bonding conditions exhibited sufficient bonding during subsequent deformation and did not reveal any debonding at the bonding interface.

Rolling up a Graphene Sheet

NARCIS (Netherlands)

Calvaresi, Matteo; Quintana, Mildred; Rudolf, Petra; Zerbetto, Francesco; Prato, Maurizio

2013-01-01

Carbon Nanotubes, CNTs, have been described as rolled-up graphene layers. Matching this concept to experiments has been a great experimental challenge for it requires a method to exfoliate graphite, generate ordered and stable dangling carbon bonds, and roll up the layer without affecting the

Roll bonding of strained aluminium

DEFF Research Database (Denmark)

Staun, Jakob M.

2003-01-01

This report investigates roll bonding of pre-strained (å ~ 4) aluminium sheets to produce high strain material from high purity aluminium (99.996%) and commercial pure aluminium (99.6%). The degree of bonding is investigated by optical microscopy and ultrasonic scanning. Under the right...... of the cross rolled volume fraction is found. To further asses this effect, and the anisotropy, it is necessary to acquire knowledge about both texture and microstructure, e.g. by TEM. Roll bonding of pre-strained aluminium is found to be a possible alternative to ARB in the quest for ultra-fine grained...

Thickness profile measuring device for rolling metal bands or sheets

International Nuclear Information System (INIS)

Campas, J.J.; Terreaux, S.

1995-01-01

Previous radiometric thickness gages were affected by insufficient water proofing and limited cooling performances for the detection subsystem (in general specially designed photodiodes). This resulted in poor reliability and life expectancy, in particular when heavy humidity and constant radiative heat are present as for hot rolling in the metal industry. This new gage design brings enhanced performances for these two factors. (D.L.). 4 refs., 3 figs

14 CFR 23.493 - Braked roll conditions.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Braked roll conditions. 23.493 Section 23.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT....493 Braked roll conditions. Under braked roll conditions, with the shock absorbers and tires in their...

An advanced dissymmetric rolling model for online regulation

Science.gov (United States)

Cao, Trong-Son

2017-10-01

Roll-bite model is employed to predict the rolling force, torque as well as to estimate the forward slip for preset or online regulation at industrial rolling mills. The rolling process is often dissymmetric in terms of work-rolls rotation speeds and diameters as well as the friction conditions at upper and lower contact surfaces between work-rolls and the strip. The roll-bite model thus must be able to account for these dissymmetries and in the same time has to be accurate and fast enough for online applications. In the present study, a new method, namely Adapted Discretization Slab Method (ADSM) is proposed to obtain a robust roll-bite model, which can take into account the aforementioned dissymmetries and has a very short response time, lower than one millisecond. This model is based on the slab method, with an adaptive discretization and a global Newton-Raphson procedure to improve the convergence speed. The model was validated by comparing with other dissymmetric models proposed in the literature, as well as Finite Element simulations and industrial pilot trials. Furthermore, back-calculation tool was also constructed for friction management for both offline and online applications. With very short CPU time, the ADSM-based model is thus attractive for all online applications, both for cold and hot rolling.

LEDs are on a roll

NARCIS (Netherlands)

Blom, P.W.M.; Mol, A.M.B. van

2011-01-01

Light-emitting diodes are more efficient than conventional lighting, but high production costs limit their uptake. Organic versions that can be produced using a cheap newspaper-style 'roll-to-roll' printing process are likely to revolutionize our lighting and signage, say Paul Blom and Ton van Mol.

14 CFR 27.493 - Braked roll conditions.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Braked roll conditions. 27.493 Section 27... AIRWORTHINESS STANDARDS: NORMAL CATEGORY ROTORCRAFT Strength Requirements Ground Loads § 27.493 Braked roll conditions. Under braked roll conditions with the shock absorbers in their static positions— (a) The limit...

14 CFR 29.493 - Braked roll conditions.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Braked roll conditions. 29.493 Section 29... AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Strength Requirements Ground Loads § 29.493 Braked roll conditions. Under braked roll conditions with the shock absorbers in their static positions— (a) The limit...

Roll-to-Roll Slot–Die Coated Organic Photovoltaic (OPV) Modules with High Geometrical Fill Factors

NARCIS (Netherlands)

Galagan, Y.; Fledderus, H.; Gorter, H.; Mannetje, H.H. 't; Shanmugam, S.; Mandamparambil, R.; Bosman, J.; Rubingh, J.M.; Teunissen, J.P.; Salem, A.; Vries, I.G. de; Andriessen, R.; Groen, W.A.

2015-01-01

Flexible semi-transparent organic photovoltaic (OPV) modules were manufactured by roll-to-roll slot–die coating of three functional layers [ZnO, photoactive layer, and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate)(PEDOT:PSS)] and either the screen printing or inkjet printing of the top

Effect of skin pass rolling reduction rate on the texture evolution of a non-oriented electrical steel after inclined cold rolling

Energy Technology Data Exchange (ETDEWEB)

Mehdi, Mehdi [CanmetMATERIALS, Natural Resources Canada, Hamilton, ON L8P 0A5 (Canada); Department of Mechanical, Automotive, and Materials Engineering, University of Windsor, Windsor, ON N9B 3P4 (Canada); He, Youliang, E-mail: youliang.he@canada.ca [CanmetMATERIALS, Natural Resources Canada, Hamilton, ON L8P 0A5 (Canada); Hilinski, Erik J. [Tempel Steel Co., Chicago, IL 60640-1020 (United States); Edrisy, Afsaneh [Department of Mechanical, Automotive, and Materials Engineering, University of Windsor, Windsor, ON N9B 3P4 (Canada)

2017-05-01

In order to promote the magnetically favourable <001>//ND texture (θ-fibre) and minimize the unfavourable <111>//ND fibre (γ-fibre) in non-oriented electrical steel, an unconventional cold rolling scheme was employed in this study, in which the cold rolling was carried out at an angle (i.e. 30°, 45°, 60°, and 90°) to the hot rolling direction (HRD). After annealing, two steel sheets (i.e. those after cold rolling at 60° and 45° to the HRD) were found to have considerably different textures from other sheets, i.e. showing the strongest and the weakest θ-fibre textures, respectively. These two sheets were then subjected to skin pass rolling to various reduction rates from 5–20% to investigate the effect of rolling reduction on the evolution of texture. It was found that during skin pass rolling, the cube texture ({001}<100>) was gradually weakened and the rotated cube orientation ({001}<110>) was strengthened. With the increase of the reduction rate, the {112}<110> orientation on the α-fibre became a major component. Upon final annealing, the cube texture was slightly restored, but the volume fraction was considerably lower than that before skin pass rolling. - Highlights: • Inclined cold rolling optimizes the textures of non-oriented electrical steels. • A 60° angle to the hot rolling direction results in the largest improvement of the favorable texture. • Skin pass rolling weakens the cube texture and promotes the {112}<110> texture. • Final annealing restores some of the cube texture and strengthens the rotated cube texture. • Low Taylor factor of the cube orientation leads to its easy deformation in skin pass rolling.

Study of deformation texture in an AZ31 magnesium alloy rolled at wide range of rolling speed and reductions

International Nuclear Information System (INIS)

Sanjari, M; Su, J; Kabir, A S; Yue, S; Tamimi, S; Hara, K; Utsunomiya, H; Petrov, R; Kestens, L

2015-01-01

The plasticity of Mg is restricted at low temperatures because: (a) only a small number of deformation mechanisms can be activated, and (b) a preferred crystallographic orientation (texture) develops in wrought alloys, especially in flat-rolled sheets. This causes problems in thin sheet processing as well as component manufacturing from the sheet. In this study, different rolling speeds from 15 to 1000 m/min were employed to warm-roll AZ31B magnesium alloy to different reductions. The results show that AZ31B sheets rolled at 15 m/min and 100 °C has fractured for reductions of more than 30% per pass. However, by increasing the rolling speed to 1000 m/min the rollability was improved significantly and the material can be rolled to reductions of more than 70% per pass. The results show that with increasing strain rate at 100°C, the splitting of basal poles was observed, indicating the activation of more contraction twins and secondary twins. (paper)

Jamming transition in a two-dimensional open granular pile with rolling resistance

Directory of Open Access Journals (Sweden)

Caio F. M. Magalhães

2014-10-01

Full Text Available We present a molecular dynamics study of the jamming/unjamming transition in two-dimensional granular piles with open boundaries. The grains are modeled by viscoelastic forces, Coulomb friction and resistance to rolling. Two models for the rolling resistance interaction were assessed: one considers a constant rolling friction coefficient, and the other one a strain dependent coefficient. The piles are grown on a finite size substrate and subsequently discharged through an orifice opened at the center of the substrate. Varying the orifice width and taking the final height of the pile after the discharge as the order parameter, one can devise a transition from a jammed regime (when the grain flux is always clogged by an arch to a catastrophic regime, in which the pile is completely destroyed by an avalanche as large as the system size. A finite size analysis shows that there is a finite orifice width associated with the threshold for the unjamming transition, no matter the model used for the microscopic interactions. As expected, the value of this threshold width increases when rolling resistance is considered, and it depends on the model used for the rolling friction. Received: 14 June 2014, Accepted: 7 October 2014; Reviewed by: R. Arévalo, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore; Edited by: L. A. Pugnaloni; DOI: http://dx.doi.org/10.4279/PIP.060007 Cite as: C F M. Magalhaes, A P F Atman, G Combe, J G Moreira, Papers in Physics 6, 060007 (2014

Rudder roll stabilization for ships

NARCIS (Netherlands)

van Amerongen, J.; van der Klugt, P.G.M.; van Nauta lemke, H.R.

1990-01-01

This paper describes the design of an autopilot for rudder roll stabilization for ships. This autopilot uses the rudder not only for course keeping but also for reduction of the roll. The system has a series of properties which make the controller design far from straightforward: the process has

Affinity labelling in situ of the bL12 protein on E. coli 70S ribosomes by means of a tRNA dialdehyde derivative.

Science.gov (United States)

Hountondji, Codjo; Créchet, Jean-Bernard; Le Caër, Jean-Pierre; Lancelot, Véronique; Cognet, Jean A H; Baouz, Soria

2017-12-01

In this report, we have used periodate-oxidized tRNA (tRNAox) as an affinity laleling reagent to demonstrate that: (i) the bL12 protein contacts the CCA-arm of P-site bound tRNA on the Escherichia coli 70S ribosomes; (ii) the stoichiometry of labelling is one molecule of tRNAox bound to one polypeptide chain of endogenous bL12; (iii) cross-linking in situ of bL12 with tRNAox on the ribosomes provokes the loss of activity; (iv) intact tRNA protects bL12 in the 70S ribosomes against cross-linking with tRNAox; (v) both tRNAox and pyridoxal 5'-phosphate (PLP) compete for the same or for proximal cross-linking site(s) on bL12 inside the ribosome; (vi) the stoichiometry of cross-linking of PLP to the recombinant E. coli bL12 protein is one molecule of PLP covalently bound per polypeptide chain; (vii) the amino acid residue of recombinant bL12 cross-linked with PLP is Lys-65; (viii) Lys-65 of E. coli bL12 corresponds to Lys-53 of eL42 which was previously shown to cross-link with P-site bound tRNAox on human 80S ribosomes in situ; (ix) finally, E. coli bL12 and human eL42 proteins display significant primary structure similarities, which argues for evolutionary conservation of these two proteins located at the tRNA-CCA binding site on eubacterial and eukaryal ribosomes. © The Authors 2017. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Look! It's Rock'n'roll!

DEFF Research Database (Denmark)

Lindelof, Anja

2007-01-01

, and dates. Consult your library or click here for more information on citing sources. -------------------------------------------------------------------------------- Anja Mølle Lindelof. (2007). Look! it's rock'n'roll! how television participated in shaping the visual genre conventions of popular music...... to personal names, capitalization, and dates. Consult your library or click here for more information on citing sources. -------------------------------------------------------------------------------- Anja Mølle Lindelof. "Look! It's Rock'n'roll! How television participated in shaping the visual genre....... Pay special attention to personal names, capitalization, and dates. Consult your library or click here for more information on citing sources. -------------------------------------------------------------------------------- TY - JOUR T1 - Look! It's Rock'n'roll! How television participated in shaping...

f(R) constant-roll inflation

Energy Technology Data Exchange (ETDEWEB)

Motohashi, Hayato [Universidad de Valencia-CSIC, Instituto de Fisica Corpuscular (IFIC), Valencia (Spain); Starobinsky, Alexei A. [L.D. Landau Institute for Theoretical Physics, RAS, Moscow (Russian Federation); National Research University Higher School of Economics, Moscow (Russian Federation)

2017-08-15

The previously introduced class of two-parametric phenomenological inflationary models in general relativity in which the slow-roll assumption is replaced by the more general, constant-roll condition is generalized to the case of f(R) gravity. A simple constant-roll condition is defined in the original Jordan frame, and exact expressions for a scalaron potential in the Einstein frame, for a function f(R) (in the parametric form) and for inflationary dynamics are obtained. The region of the model parameters permitted by the latest observational constraints on the scalar spectral index and the tensor-to-scalar ratio of primordial metric perturbations generated during inflation is determined. (orig.)

Roll-to-roll paper sensors (ROPAS); Wireless communicating sensors on paper in the logistic chain

NARCIS (Netherlands)

Rentrop, C.; Rubingh, J.E.J.M.; Lelieveld, R.; Sandberg, H.

2014-01-01

The ROPAS project (Roll-to-roll paper sensors) combines high end electronics and wireless sensors with low cost paper substrates and processing techniques that can be applied on a large scale. Paper is the next step in the printed electronics roadmap of utilising cheaper substrate materials as a

Thermal Characteristics of Plastic Film Tension in Roll-to-Roll Gravure Printed Electronics

Directory of Open Access Journals (Sweden)

Kui He

2018-02-01

Full Text Available In the printing section of a roll-to-roll gravure printed electronics machine, the plastic film tension is directly associated with the product quality. The temperature distribution of the plastic film in the printing section is non-uniform, because of the higher drying temperature and the lower room temperature. Furthermore, the drying temperature and the room temperature are not constants in industrial production. As the plastic film is sensitive to temperature, the temperature of the plastic film will affects the web tension in the printing section. In this paper, the thermal characteristics of the plastic film tension in roll-to-roll gravure printed electronics are studied in order to help to improve the product quality. First, the tension model including the factor of temperature is derived based on the law of mass conservation. Then, some simulations and experiments are carried out in order to in-depth research the effects of the drying temperature and room temperature based on the relations between system inputs and outputs. The results show that the drying temperature and room temperature have significant influences on the web tension. The research on the thermal characteristics of plastic film tension would benefit the tension control accuracy for further study.

The theory and technique of yamuna body rolling.

Science.gov (United States)

Suzuki, Satoshi

2013-09-01

[Purpose] This paper provides information about the theory and technique of Yamuna Body Rolling. In order to treat physical problems, using the specialized Yamuna Body Rolling balls, people can target superficial skin, fasciae, muscle fibers, tendons, ligaments, bones, internal organs, and the nervous system by themselves. The extraordinary effect of Yamuna Body Rolling is its multidimensional elongation of muscle fibers. In addition to the regular longitudinal elongation by the conventional stretch method, Yamuna Body Rolling enables the transversal and diagonal expansion of muscle fibers in order to move the body more dynamically. Hamstring, abdominal, and sideline routines are presented as examples for techniques of Yamuna Body Rolling. Yamuna Body Rolling can be applied to functional evaluation and therapeutic uses; therefore, it could provide many benefits in the treatment of different conditions in the medical field.

Regulation of c-Myc mRNA by L11 in Response to UV and Gamma Irradiation

Science.gov (United States)

2014-12-01

the nucleolus fol- lowed by their transport into the cytoplasm (50). This process requires coordinated transcription catalyzed by all three RNA...these RPs, including L11, are released from the nucleolus or from intact ribosomes to suppress MDM2 (68). However, whether L11 suppresses c-Myc in...centrifugation. For isolation of the nucleolus fraction, the nuclear pellets were resuspended in buffer S1 containing 0.25 M sucrose and 10 mM MgCl2, layered over

microRNA-328 inhibits cervical cancer cell proliferation and tumorigenesis by targeting TCF7L2

International Nuclear Information System (INIS)

Wang, Xuan; Xia, Ying

2016-01-01

microRNAs (miRNAs) play a vital role in tumor development and progression. In this study, we aimed to determine the expression and biological roles of miR-328 in cervical cancer and identify its direct target gene. Our data showed that miR-328 was significantly downregulated in human cervical cancer tissues and cells. Re-expression of miR-328 inhibited cervical cancer cell proliferation and colony formation in vitro and suppressed the growth of xenograft tumors in vivo. Bioinformatic analysis predicted TCF7L2 (an essential effector of canonical Wnt signaling) as a target gene of miR-328, which was confirmed by luciferase reporter assays. Enforced expression of miR-328 led to a decline in the expression of endogenous TCF7L2 in cervical cancer cells. In cervical cancer tissues, TCF7L2 protein levels were negatively correlated with miR-328 expression levels (r = −0.462, P = 0.017). Small interfering RNA-mediated knockdown of TCF7L2 significantly impaired the proliferation and colony formation of cervical cancer cells. Ectopic expression of a miRNA-resistant form of TCF7L2 significantly reversed the growth suppressive effects of miR-328 on cervical cancer cells, which was accompanied by induction of cyclin D1 expression. Taken together, our results provide first evidence for the growth suppressive activity of miR-328 in cervical cancer, which is largely ascribed to downregulation of TCF7L2. Restoration of miR-328 may have therapeutic potential in cervical cancer. -- Highlights: •miR-328 inhibits cervical cancer cell growth and tumorigenesis. •TCF7L2 is a direct target gene of miR-328 in cervical cancer. •Knockdown of TCF7L2 impairs the proliferation and colony formation of cervical cancer cells.

microRNA-328 inhibits cervical cancer cell proliferation and tumorigenesis by targeting TCF7L2

Energy Technology Data Exchange (ETDEWEB)

Wang, Xuan [Department of Gynaecology, Qilu Hospital, Shandong University, Jinan (China); Department of Gynaecology, Yantai Yuhuangding Hospital, Qingdao University School of Medicine, Yantai (China); Xia, Ying, E-mail: YingXia2006@qq.com [Department of Gynecology, Huadong Hospital, Fudan University, Shanghai, 200040 (China)

2016-06-24

microRNAs (miRNAs) play a vital role in tumor development and progression. In this study, we aimed to determine the expression and biological roles of miR-328 in cervical cancer and identify its direct target gene. Our data showed that miR-328 was significantly downregulated in human cervical cancer tissues and cells. Re-expression of miR-328 inhibited cervical cancer cell proliferation and colony formation in vitro and suppressed the growth of xenograft tumors in vivo. Bioinformatic analysis predicted TCF7L2 (an essential effector of canonical Wnt signaling) as a target gene of miR-328, which was confirmed by luciferase reporter assays. Enforced expression of miR-328 led to a decline in the expression of endogenous TCF7L2 in cervical cancer cells. In cervical cancer tissues, TCF7L2 protein levels were negatively correlated with miR-328 expression levels (r = −0.462, P = 0.017). Small interfering RNA-mediated knockdown of TCF7L2 significantly impaired the proliferation and colony formation of cervical cancer cells. Ectopic expression of a miRNA-resistant form of TCF7L2 significantly reversed the growth suppressive effects of miR-328 on cervical cancer cells, which was accompanied by induction of cyclin D1 expression. Taken together, our results provide first evidence for the growth suppressive activity of miR-328 in cervical cancer, which is largely ascribed to downregulation of TCF7L2. Restoration of miR-328 may have therapeutic potential in cervical cancer. -- Highlights: •miR-328 inhibits cervical cancer cell growth and tumorigenesis. •TCF7L2 is a direct target gene of miR-328 in cervical cancer. •Knockdown of TCF7L2 impairs the proliferation and colony formation of cervical cancer cells.

Using Light-Induced Thermocleavage in a Roll-to-Roll Process for Polymer Solar Cells

DEFF Research Database (Denmark)

Krebs, Frederik C; Norrman, Kion

2010-01-01

We report on the use of intense visible light with a narrow spectral distribution matched to the region where the conjugated polymer material absorbs to selectively heat the active material and induce thermocleavage. We show a full roll-to-roll process, leading to complete large-area polymer solar...... ion mass spectrometry, attenuated total reflectance infrared, and transmission/reflection UV−vis techniques....

Kas Eesti autoturul lõhkeb kumm? / Andres Eilart

Index Scriptorium Estoniae

Eilart, Andres

2003-01-01

Autoturu pidevalt kasvavate müügitulemuste võimalikest põhjustest Eestis ja maailmas. Tabelid ja diagramm: Euroopas valitsevad Kia ja Mazda; Uusi sõidukeid registreeritakse menukalt; Suured automüüjad kasumis. Vt. samas: Üllatused maailma autoturul

Maintenance in Railway Rolling Stock Rescheduling for Passenger Railways

NARCIS (Netherlands)

J.C. Wagenaar (Joris); L.G. Kroon (Leo)

2015-01-01

textabstractThis paper addresses the Rolling Stock Rescheduling Problem (RSRP), while taking maintenance appointments into account. After a disruption, the rolling stock of passenger trains has to be rescheduled in order to maintain a feasible rolling stock circulation. A limited number of rolling

Large Area 2D and 3D Colloidal Photonic Crystals Fabricated by a Roll-to-Roll Langmuir-Blodgett Method.

Science.gov (United States)

Parchine, Mikhail; McGrath, Joe; Bardosova, Maria; Pemble, Martyn E

2016-06-14

We present our results on the fabrication of large area colloidal photonic crystals on flexible poly(ethylene terephthalate) (PET) film using a roll-to-roll Langmuir-Blodgett technique. Two-dimensional (2D) and three-dimensional (3D) colloidal photonic crystals from silica nanospheres (250 and 550 nm diameter) with a total area of up to 340 cm(2) have been fabricated in a continuous manner compatible with high volume manufacturing. In addition, the antireflective properties and structural integrity of the films have been enhanced via the use of a second roll-to-roll process, employing a slot-die coating of an optical adhesive over the photonic crystal films. Scanning electron microscopy images, atomic force microscopy images, and UV-vis optical transmission and reflection spectra of the fabricated photonic crystals are analyzed. This analysis confirms the high quality of the 2D and 3D photonic crystals fabricated by the roll-to-roll LB technique. Potential device applications of the large area 2D and 3D colloidal photonic crystals on flexible PET film are briefly reviewed.

Development of a novel quality assurance system based on rolled-up and rolled-out radiochromic films in volumetric modulated arc therapy

International Nuclear Information System (INIS)

Park, Ji-Yeon; Lee, Jeong-Woo; Choi, Kyoung-Sik; Lee, Jung Seok; Kim, You-Hyun; Hong, Semie; Suh, Tae-Suk

2011-01-01

Purpose: To develop a cylindrical phantom with rolled-up radiochromic films and dose analysis software in the rolled-out plane for quality assurance (QA) in volumetric modulated arc therapy (VMAT). Methods: The phantom consists of an acrylic cylindrical body wrapped with radiochromic film inserted into an outer cylindrical shell of 5 cm thickness. The rolled-up films with high spatial resolution enable detection of specific dose errors along the arc trajectory of continuously irradiated and modulated beams in VMAT. The developed dose analysis software facilitates dosimetric evaluation in the rolled-up and rolled-out planes of the film; the calculated doses on the corresponding points where the rolled-up film was placed were reconstructed into a rectangular dose matrix equivalent to that of the rolled-out plane of the film. The VMAT QA system was implemented in 3 clinical cases of prostate, nasopharynx, and pelvic metastasis. Each calculated dose on the rolled-out plane was compared with measurement values by modified gamma evaluation. Detected positions of dose disagreement on the rolled-out plane were also distinguished in cylindrical coordinates. The frequency of error occurrence and error distribution were summarized in a histogram and in an axial view of rolled-up plane to intuitively identify the corresponding positions of detected errors according to the gantry angle. Results: The dose matrix reconstructed from the developed VMAT QA system was used to verify the measured dose distribution along the arc trajectory. Dose discrepancies were detected on the rolled-out plane and visualized on the calculated dose matrix in cylindrical coordinates. The error histogram obtained by gamma evaluation enabled identification of the specific error frequency at each gantry angular position. The total dose error occurring on the cylindrical surface was in the range of 5%-8% for the 3 cases. Conclusions: The developed system provides a practical and reliable QA method to

Development of a novel quality assurance system based on rolled-up and rolled-out radiochromic films in volumetric modulated arc therapy

Energy Technology Data Exchange (ETDEWEB)

Park, Ji-Yeon; Lee, Jeong-Woo; Choi, Kyoung-Sik; Lee, Jung Seok; Kim, You-Hyun; Hong, Semie; Suh, Tae-Suk [Department of Biomedical Engineering and Research Institute of Biomedical Engineering, College of Medicine, Catholic University of Korea, Seoul 137-701 (Korea, Republic of); Research Institute of Health Science, College of Health Science, Korea University, Seoul 136-703 (Korea, Republic of) and Department of Radiation Oncology, Konkuk University Medical Center, Seoul 143-729 (Korea, Republic of); Department of Radiation Oncology, Anyang SAM Hospital, Anyang 430-733 (Korea, Republic of) and Department of Biomedical Engineering and Research Institute of Biomedical Engineering, College of Medicine, Catholic University of Korea, Seoul 137-701 (Korea, Republic of); Department of Radiation Oncology, Anyang SAM Hospital, Anyang 430-733 (Korea, Republic of); Department of Radiologic Science, College of Health Science, Korea University, Seoul 136-703 (Korea, Republic of); Department of Radiation Oncology, Konkuk University Medical Center, Seoul 143-729 (Korea, Republic of); Department of Biomedical Engineering and Research Institute of Biomedical Engineering, College of Medicine, Catholic University of Korea, Seoul 137-701 (Korea, Republic of)

2011-12-15

Purpose: To develop a cylindrical phantom with rolled-up radiochromic films and dose analysis software in the rolled-out plane for quality assurance (QA) in volumetric modulated arc therapy (VMAT). Methods: The phantom consists of an acrylic cylindrical body wrapped with radiochromic film inserted into an outer cylindrical shell of 5 cm thickness. The rolled-up films with high spatial resolution enable detection of specific dose errors along the arc trajectory of continuously irradiated and modulated beams in VMAT. The developed dose analysis software facilitates dosimetric evaluation in the rolled-up and rolled-out planes of the film; the calculated doses on the corresponding points where the rolled-up film was placed were reconstructed into a rectangular dose matrix equivalent to that of the rolled-out plane of the film. The VMAT QA system was implemented in 3 clinical cases of prostate, nasopharynx, and pelvic metastasis. Each calculated dose on the rolled-out plane was compared with measurement values by modified gamma evaluation. Detected positions of dose disagreement on the rolled-out plane were also distinguished in cylindrical coordinates. The frequency of error occurrence and error distribution were summarized in a histogram and in an axial view of rolled-up plane to intuitively identify the corresponding positions of detected errors according to the gantry angle. Results: The dose matrix reconstructed from the developed VMAT QA system was used to verify the measured dose distribution along the arc trajectory. Dose discrepancies were detected on the rolled-out plane and visualized on the calculated dose matrix in cylindrical coordinates. The error histogram obtained by gamma evaluation enabled identification of the specific error frequency at each gantry angular position. The total dose error occurring on the cylindrical surface was in the range of 5%-8% for the 3 cases. Conclusions: The developed system provides a practical and reliable QA method to

Analytical method for establishing indentation rolling resistance

Science.gov (United States)

Gładysiewicz, Lech; Konieczna, Martyna

2018-01-01

Belt conveyors are highly reliable machines able to work in special operating conditions. Harsh environment, long distance of transporting and great mass of transported martials are cause of high energy usage. That is why research in the field of belt conveyor transportation nowadays focuses on reducing the power consumption without lowering their efficiency. In this paper, previous methods for testing rolling resistance are described, and new method designed by authors was presented. New method of testing rolling resistance is quite simple and inexpensive. Moreover it allows to conduct the experimental tests of the impact of different parameters on the value of indentation rolling resistance such as core design, cover thickness, ambient temperature, idler travel frequency, or load value as well. Finally results of tests of relationship between rolling resistance and idler travel frequency and between rolling resistance and idler travel speed was presented.

Rolling motions in an inner spiral arm

International Nuclear Information System (INIS)

Strauss, F.M.; Poeppel, W.

1976-01-01

Hydrogen line observations made at low galactic latitudes for l=318degree, 326degree, 334degree, and 337degree show the presence of velocity gradients in latitude in the nearest inner spiral arm, similar to those found by other observations in different regions. Maximum velocity change is about 10 km s -1 for l=337degree. By generating synthetic line profiles constructed from a model spiral arm, several possible causes of these ''rolling motions'' were studied, such as a vertical displacement or a tilt of the arm (which failed to account for the observations) and rotation or shearing in the arm. It was futher shown that a typical arm can maintain such a motion (approx. =75 km s -1 kpc -1 ) with its own gravitational potential. The results are used to study the origin and tilt of Gould's Belt

Phase transformation kinetics in rolled U-10 wt. % Mo foil: Effect of post-rolling heat treatment and prior γ-UMo grain size

Energy Technology Data Exchange (ETDEWEB)

Jana, Saumyadeep; Overman, Nicole; Varga, Tamas; Lavender, Curt; Joshi, Vineet V.

2017-12-01

The effect of sub-eutectoid heat treatment on the phase transformation behavior in rolled U-10 wt.percent Mo (U10Mo) foils was systematically investigated. The as-cast 5 mm thick foils were initially homogenized at 900 degrees C for 48 hours and were hot rolled to 2 mm and later cold rolled down to 0.2 mm. Three starting microstructures were evaluated: (i) hot- + cold-rolled to 0.2 mm (as-rolled condition), (ii) hot- + cold-rolled to 0.2 mm + annealed at 700 deg. C for 1 hour, and (iii) hot- + cold-rolled to 0.2 mm + annealed at 1000 deg. C for 60 hours. U10Mo rolled foils went through various degrees of decomposition when subjected to the sub-eutectoid heat-treatment step and formed a lamellar microstructure through a cellular reaction mostly along the previous γ-UMo grain boundaries.

Development of an aerostatic bearing system for roll-to-roll printed electronics

Science.gov (United States)

Chen, Shasha; Chen, Weihai; Liu, Jingmeng; Chen, Wenjie; Jin, Yan

2018-06-01

Roll-to-roll printed electronics is proved to be an effective way to fabricate electrical devices on various substrates. High precision overlay alignment plays a key role to create multi-layer electrical devices. Multiple rollers are adopted to support and transport the substrate web. In order to eliminate the negative effect of the machining error and assembling error of the roller, a whole roll-to-roll system including two aerostatic bearing devices with arrayed restrictors is proposed in this paper. Different to the conventional roller, the aerostatic bearing device can create a layer of air film between the web and the device to realize non-contact support and transport. Based on simplified Navier–Stokes equations, the theoretical model of the air film is established. Moreover, the pressure distribution of the whole flow field and single restrictor in different positions are modeled by conducting numerical simulation with computational fluid dynamics (CFD) software FLUENT. The load capacity curves and stiffness curves are generated to provide guidance for optimizing the structure of the device. A prototype of the aerostatic bearing system is set up and the experiment tests are carried out. For the proposed aerostatic bearing roller with a diameter of 100 mm and length of 200 mm, the experimental results show the aerostatic bearing method can achieve the position accuracy in a range of 1 μm in the vertical direction of the web, which is much better than that using existing methods.

Effects of hot rolled microstructure after twin-roll casting on microstructure, texture and magnetic properties of low silicon non-oriented electrical steel

International Nuclear Information System (INIS)

Liu, Hai-Tao; Wang, Yin-Ping; An, Ling-Zi; Wang, Zhao-Jie; Hou, Dao-Yuan; Chen, Jun-Mou; Wang, Guo-Dong

2016-01-01

In this work, a 0.71 wt%Si+0.44 wt%Al as-cast strip was produced by novel twin-roll casting. Some as-cast samples were respectively reheated and hot rolled at different temperatures in order to obtain different microstructure prior to cold rolling and annealing. The effects of the hot rolled microstructure on microstructure, texture evolution and magnetic properties were investigated in detail. A coarse deformed microstructure with λ-fiber texture was formed after hot rolling at 850–1050 °C, finally leading to an inhomogeneous recrystallization microstructure with strong λ-fiber, Goss and extremely weak γ-fiber texture. By contrast, a fine transformed microstructure was formed after hot rolling at 1150–1250 °C, finally leading to a fine and homogeneous recrystallization microstructure with stronger α-fiber, γ-fiber and much weaker λ-fiber texture. It should be noted that both the magnetic induction and core loss non-monotonically decreased or increased according to the hot rolling temperature. The unfavorable α-fiber and γ-fiber textures in the annealed sheets were much weaker than those of the conventional products regardless of the hot rolling temperature, thus contributing to a much higher magnetic induction. However, the average grain size in the annealed sheets was much lower than those of the conventional products regardless of the hot rolling temperature, thus leading to a higher core loss except the case of 1050 °C. Hence, it is underscored that better integrated magnetic properties than those of the conventional products can be obtained by optimizing the hot rolled microstructure to produce final desirable recrystallization microstructure and texture. - Highlights: • Non-oriented silicon steel was fabricated using twin-roll casting route. • Microstructure and texture evolution were clarified. • Effects of the hot rolled microstructure were investigated in detail. • Formation mechanism of the recrystallization texture was explored. �

Effects of hot rolled microstructure after twin-roll casting on microstructure, texture and magnetic properties of low silicon non-oriented electrical steel

Energy Technology Data Exchange (ETDEWEB)

Liu, Hai-Tao, E-mail: liuht@ral.neu.edu.cn; Wang, Yin-Ping; An, Ling-Zi; Wang, Zhao-Jie; Hou, Dao-Yuan; Chen, Jun-Mou; Wang, Guo-Dong

2016-12-15

In this work, a 0.71 wt%Si+0.44 wt%Al as-cast strip was produced by novel twin-roll casting. Some as-cast samples were respectively reheated and hot rolled at different temperatures in order to obtain different microstructure prior to cold rolling and annealing. The effects of the hot rolled microstructure on microstructure, texture evolution and magnetic properties were investigated in detail. A coarse deformed microstructure with λ-fiber texture was formed after hot rolling at 850–1050 °C, finally leading to an inhomogeneous recrystallization microstructure with strong λ-fiber, Goss and extremely weak γ-fiber texture. By contrast, a fine transformed microstructure was formed after hot rolling at 1150–1250 °C, finally leading to a fine and homogeneous recrystallization microstructure with stronger α-fiber, γ-fiber and much weaker λ-fiber texture. It should be noted that both the magnetic induction and core loss non-monotonically decreased or increased according to the hot rolling temperature. The unfavorable α-fiber and γ-fiber textures in the annealed sheets were much weaker than those of the conventional products regardless of the hot rolling temperature, thus contributing to a much higher magnetic induction. However, the average grain size in the annealed sheets was much lower than those of the conventional products regardless of the hot rolling temperature, thus leading to a higher core loss except the case of 1050 °C. Hence, it is underscored that better integrated magnetic properties than those of the conventional products can be obtained by optimizing the hot rolled microstructure to produce final desirable recrystallization microstructure and texture. - Highlights: • Non-oriented silicon steel was fabricated using twin-roll casting route. • Microstructure and texture evolution were clarified. • Effects of the hot rolled microstructure were investigated in detail. • Formation mechanism of the recrystallization texture was explored. �

Low Band Gap Polymers for Roll-to-Roll Coated Organic Photovoltaics – Design, Synthesis and Characterization

DEFF Research Database (Denmark)

Bundgaard, Eva; Hagemann, Ole; Jørgensen, Mikkel

2011-01-01

In this paper we present the design and synthesis of 25 new low band gap polymers. The polymers were characterized by UV-vis spectroscopy which showed optical band gaps of 2.0–0.9 eV. The polymers which were soluble enough were applied in organic photovoltaics, both small area devices with a spin...... coated active layer and in large area modules where all layers including the active layer were either roll-to-roll coated or printed. These experiments showed that the design of polymers compatible with roll-toroll coating is not straightforward and that there are various issues such as donor...

Roll-to-roll fabrication of a low-reflectance transparent conducting oxide film with subwavelength structures

Science.gov (United States)

Chou, Ta-Hsin; Cheng, Kuei-Yuan; Hsieh, Chih-Wei; Takaya, Yasuhiro

2012-04-01

The transparent conducting oxide (TCO) film is a significant component in flat panel display, e-paper and touch panel. The tin-doped indium oxide (ITO) material is one of the most popular TCOs. However, ITO has high refractive index, so the phenomenon of high-reflectance limits the wide use of ITO. In this study, the structure and mass production process of new low-reflectance TCO film is verified. Laser interference lithography and the roll-to-roll UV embossing process are used to fabricate subwavelength structures on PET film; then ITO was deposited on structures by roll-to-roll sputtering. When the dimension of structures reaches 300 nm pitch, the optical reflectance and electrical performance of film are reduced to 8.1% at wavelength 550 nm and its transmittance rate is 84.3% at the same wavelength, and the sheet resistance of this film is 50.44 Ω/□. This result indicates that the new TCO proposed in this study is suitable for touch panel and other display applications.

Analytical method for establishing indentation rolling resistance

Directory of Open Access Journals (Sweden)

Gładysiewicz Lech

2018-01-01

Full Text Available Belt conveyors are highly reliable machines able to work in special operating conditions. Harsh environment, long distance of transporting and great mass of transported martials are cause of high energy usage. That is why research in the field of belt conveyor transportation nowadays focuses on reducing the power consumption without lowering their efficiency. In this paper, previous methods for testing rolling resistance are described, and new method designed by authors was presented. New method of testing rolling resistance is quite simple and inexpensive. Moreover it allows to conduct the experimental tests of the impact of different parameters on the value of indentation rolling resistance such as core design, cover thickness, ambient temperature, idler travel frequency, or load value as well. Finally results of tests of relationship between rolling resistance and idler travel frequency and between rolling resistance and idler travel speed was presented.

An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L

Directory of Open Access Journals (Sweden)

Kaur Jatinder

2010-05-01

Full Text Available Abstract Background RNA quality and quantity is sometimes unsuitable for cDNA library construction, from plant seeds rich in oil, polysaccharides and other secondary metabolites. Seeds of jatropha (Jatropha curcas L. are rich in fatty acids/lipids, storage proteins, polysaccharides, and a number of other secondary metabolites that could either bind and/or co-precipitate with RNA, making it unsuitable for downstream applications. Existing RNA isolation methods and commercial kits often fail to deliver high-quality total RNA from immature jatropha seeds for poly(A+ RNA purification and cDNA synthesis. Findings A protocol has been developed for isolating good quality total RNA from immature jatropha seeds, whereby a combination of the CTAB based RNA extraction method and a silica column of a commercial plant RNA extraction kit is used. The extraction time was reduced from two days to about 3 hours and the RNA was suitable for poly(A+ RNA purification, cDNA synthesis, cDNA library construction, RT-PCR, and Northern hybridization. Based on sequence information from selected clones and amplified PCR product, the cDNA library seems to be a good source of full-length jatropha genes. The method was equally effective for isolating RNA from mustard and rice seeds. Conclusions This is a simple CTAB + silica column method to extract high quality RNA from oil rich immature jatropha seeds that is suitable for several downstream applications. This method takes less time for RNA extraction and is equally effective for other tissues where the quality and quantity of RNA is highly interfered by the presence of fatty acids, polysaccharides and polyphenols.

Radiometric study of creep in ingot rolling

International Nuclear Information System (INIS)

Kubicek, P.; Zamyslovsky, Z.; Uherek, J.

The radiometric study of creep during ingot rolling performed in the rolling mill of the Vitkovice Iron and Steel Works and the first results are described. Selected sites in 3 to 8 ton ingots were labelled with 2 to 3.7x10 5 Bq of 60 Co and after rolling into blocks, the transposition of the labelled sites of the ingots was investigated. The results indicate creep during rolling, local extension in certain sites under study and help to determine the inevitable bottom crop incurred in the forming. Finally, the requirements put on the radiometric apparatus for the next stages of technological research are presented. (author)

Research upon the quality assurance of the rolling-mill rolls and the variation boundaries of the chemical composition

Directory of Open Access Journals (Sweden)

Kiss, I.

2008-08-01

Full Text Available The cast-iron rolls must present higher hardness at the rolling surface and lower in the core and the necks, adequate with mechanical resistance and in the high work temperature. If in the zone of the rolling surface, the hardness is guarantied by the irons structure, through the cementite quantities, the core of rolls must contain graphite, to assure this property. Starting from the lamination equipments aspects, from the form of rolls, of the technological interest zones and the structure, which assures the exploitation property, it was establish, through modeling, to the mathematical description of a direct influences, and in final, through successive determinations, to an optimum. One of the parameters, which are determined the structure of the irons destined for rolls casting, is the chemical composition, which guaranties the exploitation properties of the each roll in the stand of rolling mill. The realization of optimum chemical compositions of the cast-iron can constitute a technical efficient way to assure the exploitation properties, the material from which the rolling mills rolls are manufactured having an important role in this sense. Although the manufacture of rolls is in continuously perfecting, the requirements for superior quality rolls are not yet completely satisfied, in many cases, the absence of quality rolls preventing the realization of quality laminates or the realization of productivities of which rolling mills are capable. This paper presents an analysis of the main alloying elements from chemical composition, the influences upon the mechanical properties of the cast-iron rolls, and presents also some graphical addenda. Using the Matlab calculation and graphical programs we determinate some correlations between the hardness (on the working surface and on necks and the chemical composition. Using the double and triple correlations is really helpful in the foundry practice, as it allows us to determine variation

76 FR 34101 - Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil, Japan, and Russia

Science.gov (United States)

2011-06-10

...] Hot-Rolled Flat-Rolled Carbon-Quality Steel Products From Brazil, Japan, and Russia Determinations On...-quality steel products from Russia would be likely to lead to continuation or recurrence of material injury to an industry in the United States within a reasonably foreseeable time. The Commission further...

Die Rolle des Gerichtsdolmetschers - Experte oder notwendiges Übel?

DEFF Research Database (Denmark)

Christensen, Tina Paulsen

2012-01-01

Die Zahl der gedolmetschten Gerichtsverhandlungen steigt weiterhin in vielen Rechtssystemen, weshalb es immer wichtiger wird, dass Rechtssysteme über qualifizierte Dolmetscher verfügen. In Dänemark begegnen Richter, die Rezipienten von Gerichtsdolmetschleistungen sind, sowohl professionellen als...... auch nicht- oder semi-professionellen Gerichtsdolmetschern. Dies lässt vermuten, dass die Richter in der Praxis Dolmetschleistungen von sehr unterschiedlicher Qualität erleben und vermutlich kein einheitliches Bild von der Rolle des Gerichtsdolmetschers haben. Ziel der Studie, die ich in diesem Beitrag...

Pipe Rolling from Continuous Cast Metal

International Nuclear Information System (INIS)

Zhordania, I.; Chkhartishvili, I.; Lordkipanidze, J.; Melashvili, Z.; Papava, K.; Khundadze, K.

2007-01-01

The approach to manufacturing of high quality pipes as a result of solid and hollow billet rolling from continuous cast metal is shown. Optimal parameters of piercing, temperature of piercing and piercing rolling mill rollers speed have been experimentally established. (author)

Efficient Circulation of Railway Rolling Stock

NARCIS (Netherlands)

A. Alfieri (Arianna); R. Groot (Rutger); L.G. Kroon (Leo); A. Schrijver (Lex)

2002-01-01

textabstractRailway rolling stock (locomotives, carriages, and train units) is one of the most significant cost sources for operatorsof passenger trains, both public and private. Rolling stock costsare due to material acquisition, power supply, and material maintenance. The efficient circulation of

Relvakogujad sõjaajalooklubist Front Line vahistati / Kadri Ratt

Index Scriptorium Estoniae

Ratt, Kadri

2009-01-01

Kaitsepolitsei võttis vahi alla 9 meest, keda kahtlustatakse suures koguses lõhkeainete, lõhkekehade ja tulirelvade ebaseaduslikus käitlemises. 6 meest neist kuuluvad sõjaajalooklubisse Front Line

A round robin study of flexible large-area roll-to-roll processed polymer solar cell modules

DEFF Research Database (Denmark)

Krebs, Frederik C; Gevorgyan, Suren; Gholamkhass, Bobak

2009-01-01

A round robin for the performance of roll-to-roll coated flexible large-area polymer solar-cell modules involving 18 different laboratories in Northern America, Europe and Middle East is presented. The study involved the performance measurement of the devices at one location (Risø DTU) followed b...

Large-scale roll-to-roll photonic sintering of flexo printed silver nanoparticle electrodes

DEFF Research Database (Denmark)

Hösel, Markus; Krebs, Frederik C

2012-01-01

In this report we employ static and roll-to-roll (R2R) photonic sintering processes on flexo printed silver nanoparticle-based electrode structures with a heat-sensitive 60 mm thin barrier foil as a substrate. We use large area electrode structures to visualize the increased optical footprint...... as the nanoparticles are already sintered. The advantage of single exposure is the ability to produce higher R2R processing speeds without overlapping, which is shown in the form of theoretical calculations....

Controlled Microwave Heating Accelerates Rolling Circle Amplification.

Directory of Open Access Journals (Sweden)

Takeo Yoshimura

Full Text Available Rolling circle amplification (RCA generates single-stranded DNAs or RNA, and the diverse applications of this isothermal technique range from the sensitive detection of nucleic acids to analysis of single nucleotide polymorphisms. Microwave chemistry is widely applied to increase reaction rate as well as product yield and purity. The objectives of the present research were to apply microwave heating to RCA and indicate factors that contribute to the microwave selective heating effect. The microwave reaction temperature was strictly controlled using a microwave applicator optimized for enzymatic-scale reactions. Here, we showed that microwave-assisted RCA reactions catalyzed by either of the four thermostable DNA polymerases were accelerated over 4-folds compared with conventional RCA. Furthermore, the temperatures of the individual buffer components were specifically influenced by microwave heating. We concluded that microwave heating accelerated isothermal RCA of DNA because of the differential heating mechanisms of microwaves on the temperatures of reaction components, although the overall reaction temperatures were the same.

Controlled Microwave Heating Accelerates Rolling Circle Amplification.

Science.gov (United States)

Yoshimura, Takeo; Suzuki, Takamasa; Mineki, Shigeru; Ohuchi, Shokichi

2015-01-01

Rolling circle amplification (RCA) generates single-stranded DNAs or RNA, and the diverse applications of this isothermal technique range from the sensitive detection of nucleic acids to analysis of single nucleotide polymorphisms. Microwave chemistry is widely applied to increase reaction rate as well as product yield and purity. The objectives of the present research were to apply microwave heating to RCA and indicate factors that contribute to the microwave selective heating effect. The microwave reaction temperature was strictly controlled using a microwave applicator optimized for enzymatic-scale reactions. Here, we showed that microwave-assisted RCA reactions catalyzed by either of the four thermostable DNA polymerases were accelerated over 4-folds compared with conventional RCA. Furthermore, the temperatures of the individual buffer components were specifically influenced by microwave heating. We concluded that microwave heating accelerated isothermal RCA of DNA because of the differential heating mechanisms of microwaves on the temperatures of reaction components, although the overall reaction temperatures were the same.

Magnon Inflation: Slow Roll with Steep Potentials

CERN Document Server

Adshead, Peter; Burgess, C P; Hayman, Peter; Patil, Subodh P

2016-01-01

We find multi-scalar effective field theories (EFTs) that can achieve a slow inflationary roll despite having a scalar potential that does not satisfy the usual slow-roll condition (d V)^2 << V^2/Mp^2. They evade the usual slow-roll conditions on $V$ because their kinetic energies are dominated by single-derivative terms rather than the usual two-derivative terms. Single derivatives dominate during slow roll and so do not require a breakdown of the usual derivative expansion that underpins calculational control in much of cosmology. The presence of such terms requires some sort of UV Lorentz-symmetry breaking during inflation (besides the usual cosmological breaking). Chromo-natural inflation provides an example of a UV theory that can generate the multi-field single-derivative terms we consider, and we argue that the EFT we find indeed captures the slow-roll conditions for the background evolution for Chromo-natural inflation. We also show that our EFT can be understood as a multi-field generalization ...

Microstructure and mechanical properties of Mg-Al-Mn-Ca alloy sheet produced by twin roll casting and sequential warm rolling

International Nuclear Information System (INIS)

Wang Yinong; Kang, Suk Bong; Cho, Jaehyung

2011-01-01

Research highlights: → This work, taking AM30 + 0.2Ca alloy as experimental material, will provide some new information as follows: one is microstructural difference between twin roll cast and ingot cast AM31-0.2Ca alloy. The other is the comparison of tensile properties after warm rolling and annealing. Suggesting the possibility of the development of wrought magnesium alloy sheets by strip casting. - Abstract: Microstructural evolution and mechanical properties of twin roll cast (TRC) Mg-3.3 wt.%Al-0.8 wt.%Mn-0.2 wt.%Ca (AM31 + 0.2Ca) alloy strip during warm rolling and subsequent annealing were investigated in this paper. The as-TRC alloy strip shows columnar dendrites in surface and equiaxed dendrites in center regions, as well as finely dispersed primary Al 8 Mn 5 particles on interdendritic boundaries which result in the beneficial effect on microstructural refinement of strip casting. The warm rolled sheets show intensively deformed band or shear band structures, as well as finely and homogeneously dispersed Al-Mn particles. No evident dynamic recrystallization (DRX) takes place during warm rolling process, which is more likely attributed to the finely dispersed particle and high solid solution of Al and Mn atoms in α-Mg matrix. After annealing at 350 deg. C for 1 h, the warm rolled TRC sheets show fine equiaxed grains around 7.8 μm in average size. It has been shown that the present TRC alloy sheet has superior tensile strength and comparative elongation compared to commercial ingot cast (IC) one, suggesting the possibility of the development of wrought magnesium alloy sheets by twin roll strip casting processing. The microstructural evolution during warm rolling and subsequent annealing as well as the resulting tensile properties were analyzed and discussed.

ROLLING PROCESS WITH OHSAS AND TEXTURE FORMATION– A REVIEW

Directory of Open Access Journals (Sweden)

P. CHANDRAMOHAN

2009-03-01

Full Text Available Rolling is a mechanical treatment, which plays an important part in the processing of ferrous and nonferrous alloys. Texturing is an important phenomenon that occurs after rolling process. Preferred orientation increases the strength of the material enormously. Hence the research is focused on the rolling studies and the texture formation, which occurs after rolling process. This review mainly focuses on rolling process carried out in different alloys. It also highlights the analysis made on various rolling parameters for improving the mechanical properties. Texture studies carried on various ferrous and non-ferrous alloys; particularly in nitrogen alloyed duplex stainless steel is discussed. Finally the need for implementation of occupational health and safety during a thermomechanical treatment is also discussed. The state of art in this field is encouraging and showing positive signs of commercializing rolled nitrogen alloyed duplex stainless steel after proper texture control.

Flexible indium zinc oxide/Ag/indium zinc oxide multilayer electrode grown on polyethersulfone substrate by cost-efficient roll-to-roll sputtering for flexible organic photovoltaics

International Nuclear Information System (INIS)

Park, Yong-Seok; Kim, Han-Ki

2010-01-01

The authors describe the preparation and characteristics of flexible indium zinc oxide (IZO)-Ag-IZO multilayer electrodes grown on flexible polyethersulfone (PES) substrates using a roll-to-roll sputtering system for use in flexible organic photovoltaics. By the continuous roll-to-roll sputtering of the bottom IZO, Ag, and top IZO layers at room temperature, they were able to fabricate a high quality IZO-Ag-IZO multilayer electrode with a sheet resistance of 6.15 ε/square, optical transmittance of 87.4%, and figure of merit value of 42.03x10 -3 Ω -1 on the PES substrate. In addition, the IZO-Ag-IZO multilayer electrode exhibited superior flexibility to the roll-to-roll sputter grown single ITO electrode due to the existence of a ductile Ag layer between the IZO layers and stable amorphous structure of the IZO film. Furthermore, the flexible organic solar cells (OSCs) fabricated on the roll-to-roll sputter grown IZO-Ag-IZO electrode showed higher power efficiency (3.51%) than the OSCs fabricated on the roll-to-roll sputter grown single ITO electrode (2.67%).

Interlayer adhesion in roll-to-roll processed flexible inverted polymer solar cells

DEFF Research Database (Denmark)

Dupont, Stephanie R.; Oliver, Mark; Krebs, Frederik C

2012-01-01

demonstrate how a thin-film adhesion technique can be applied to flexible organic solar cells to obtain quantitative adhesion values. For the P3HT:PCBM-based BHJ polymer solar cells, the interface of the BHJ with the conductive polymer layer PEDOT:PSS was found to be the weakest. The adhesion fracture energy......The interlayer adhesion of roll-to-roll processed flexible inverted P3HT:PCBM bulk heterojunction (BHJ) polymer solar cells is reported. Poor adhesion between adjacent layers may result in loss of device performance from delamination driven by the thermomechanical stresses in the device. We...... energies was observed....

Roll-to-roll processed polymer tandem solar cells partially processed from water

DEFF Research Database (Denmark)

Larsen-Olsen, Thue Trofod; Andersen, Thomas Rieks; Andreasen, Birgitta

2012-01-01

Large area polymer tandem solar cells completely processed using roll-to-roll (R2R) coating and printing techniques are demonstrated. A stable tandem structure was achieved by the use of orthogonal ink solvents for the coating of all layers, including both active layers. Processing solvents...... included water, alcohols and chlorobenzene. Open-circuit voltages close to the expected sum of sub cell voltages were achieved, while the overall efficiency of the tandem cells was found to be limited by the low yielding back cell, which was processed from water based ink. Many of the challenges associated...

Interlaboratory indoor ageing of roll-to-roll and spin coated organic photovoltaic devices: Testing the ISOS tests

DEFF Research Database (Denmark)

Gevorgyan, Suren A.; Corazza, Michael; Madsen, Morten Vesterager

2014-01-01

to roll-to-roll production. Furthermore, the reproducibility of current–voltage (IV) measurement and preconditioning (light soaking treatments) are addressed. Additionally, the inter-comparison of the degradation rates of the samples aged under three different dark test conditions (ambient, dry/heat, damp...

High throughput in situ scattering of roll-to-roll coated functional polymer films

DEFF Research Database (Denmark)

Andreasen, Jens Wenzel

2017-01-01

The development of conjugated polymers for organic electronics and photovoltaics has relied heavily on advanced X-ray scattering techniques almost since the earliest studies in the field. Almost from the beginning, structural studies focused on how the polymers self-organize in thin films......, and the relation between chemical configuration of the polymer, structure and performance. This chapter presents the latest developments where structural analysis is applied as in situ characterization of structure formation during roll-to-roll coating of photoactive layers for solar cells....

Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference

Directory of Open Access Journals (Sweden)

Malgorzata Sierant

2011-01-01

Full Text Available RNA interference (RNAi technology provides a powerful molecular tool to reduce an expression of selected genes in eukaryotic cells. Short interfering RNAs (siRNAs are the effector molecules that trigger RNAi. Here, we describe siRNAs that discriminate between the wild type and mutant (1174 C→G alleles of human Presenilin1 gene (PSEN1. This mutation, resulting in L392V PSEN1 variant, contributes to early onset familial Alzheimer's disease. Using the dual fluorescence assay, flow cytometry and fluorescent microscopy we identified positions 8th–11th, within the central part of the antisense strand, as the most sensitive to mismatches. 2-Thiouridine chemical modification introduced at the 3′-end of the antisense strand improved the allele discrimination, but wobble base pairing adjacent to the mutation site abolished the siRNA activity. Our data indicate that siRNAs can be designed to discriminate between the wild type and mutant alleles of genes that differ by just a single nucleotide.

Movement patterns of limb coordination in infant rolling.

Science.gov (United States)

Kobayashi, Yoshio; Watanabe, Hama; Taga, Gentaro

2016-12-01

Infants must perform dynamic whole-body movements to initiate rolling, a key motor skill. However, little is known regarding limb coordination and postural control in infant rolling. To address this lack of knowledge, we examined movement patterns and limb coordination during rolling in younger infants (aged 5-7 months) that had just begun to roll and in older infants (aged 8-10 months) with greater rolling experience. Due to anticipated difficulty in obtaining measurements over the second half of the rolling sequence, we limited our analysis to the first half. Ipsilateral and contralateral limbs were identified on the basis of rolling direction and were classified as either a stationary limb used for postural stability or a moving limb used for controlled movement. We classified the observed movement patterns by identifying the number of stationary limbs and the serial order of combinational limb movement patterns. Notably, older infants performed more movement patterns that involved a lower number of stationary limbs than younger infants. Despite the wide range of possible movement patterns, a small group of basic patterns dominated in both age groups. Our results suggest that the fundamental structure of limb coordination during rolling in the early acquisition stages remains unchanged until at least 8-10 months of age. However, compared to younger infants, older infants exhibited a greater ability to select an effective rotational movement by positioning themselves with fewer stationary limbs and performing faster limb movements.

New Numerical Solution of von Karman Equation of Lengthwise Rolling

Directory of Open Access Journals (Sweden)

Rudolf Pernis

2015-01-01

Full Text Available The calculation of average material contact pressure to rolls base on mathematical theory of rolling process given by Karman equation was solved by many authors. The solutions reported by authors are used simplifications for solution of Karman equation. The simplifications are based on two cases for approximation of the circular arch: (a by polygonal curve and (b by parabola. The contribution of the present paper for solution of two-dimensional differential equation of rolling is based on description of the circular arch by equation of a circle. The new term relative stress as nondimensional variable was defined. The result from derived mathematical models can be calculated following variables: normal contact stress distribution, front and back tensions, angle of neutral point, coefficient of the arm of rolling force, rolling force, and rolling torque during rolling process. Laboratory cold rolled experiment of CuZn30 brass material was performed. Work hardening during brass processing was calculated. Comparison of theoretical values of normal contact stress with values of normal contact stress obtained from cold rolling experiment was performed. The calculations were not concluded with roll flattening.

Experimental determination of heat transfer coefficients in roll bite and air cooling for computer simulations of 1100 MPa carbon steel rolling

Science.gov (United States)

Leinonen, Olli; Ilmola, Joonas; Seppälä, Oskari; Pohjonen, Aarne; Paavola, Jussi; Koskenniska, Sami; Larkiola, Jari

2018-05-01

In modeling of hot rolling pass schedules the heat transfer phenomena have to be known. Radiation to ambient, between rolls and a steel slab as well as heat transfer in contacts must be considered to achieve accurate temperature distribution and thereby accurate material behavior in simulations. Additional heat is generated by friction between the slab and the work roll and by plastic deformation. These phenomena must be taken into account when the effective heat transfer coefficient is determined from experimental data. In this paper we determine the effective heat transfer coefficient at the contact interface and emissivity factor of slab surface for 1100MPa strength carbon steel for hot rolling simulations. Experimental pilot rolling test were carried out and slab temperatures gathered right below the interface and at the mid thickness of the slab. Emissivity factor tests were carried out in the same manner but without rolling. Experimental data is utilized to derive contact heat transfer coefficient at the interface and emissivity factor of slab surface. Pilot rolling test is reproduced in FE-analysis to further refine the heat transfer coefficient and emissivity factor. Material mechanical properties at rolling temperatures were determined by Gleeble™ thermo-mechanical simulator and IDS thermodynamic-kinetic-empirical software.

Rolling at small scales

DEFF Research Database (Denmark)

Nielsen, Kim L.; Niordson, Christian F.; Hutchinson, John W.

2016-01-01

The rolling process is widely used in the metal forming industry and has been so for many years. However, the process has attracted renewed interest as it recently has been adapted to very small scales where conventional plasticity theory cannot accurately predict the material response. It is well....... Metals are known to be stronger when large strain gradients appear over a few microns; hence, the forces involved in the rolling process are expected to increase relatively at these smaller scales. In the present numerical analysis, a steady-state modeling technique that enables convergence without...

Edge sealing for low cost stability enhancement of roll-to-roll processed flexible polymer solar cell modules

DEFF Research Database (Denmark)

Tanenbaum, David M.; Dam, Henrik Friis; Rösch, R.

2012-01-01

Fully roll-to-roll processed polymer solar cell modules were prepared, characterized, and laminated. Cell modules were cut from the roll and matched pairs were selected, one module with exposed cut edges, the other laminated again with the same materials and adhesive sealing fully around the cut...... edges. The edge sealing rim was 10 mm wide. Cell modules were characterized by periodic measurements of IV curves over extended periods in a variety of conditions, as well as by a variety of spatial imaging techniques. Data show significant stability benefits of the edge sealing process. The results...

Effects of the process temperature and rolling speed on the thermal roll-to-roll imprint lithography of flexible polycarbonate film

International Nuclear Information System (INIS)

Sohn, Ki-Ju; Lee, Woo Il; Park, Jae Hong; Jang, Hyun-Ik; Lee, Dong-Eon

2013-01-01

Thermal roll-to-roll imprint lithography (R2RIL) is a simple and low-cost process for the mass production of micro/nanopatterns. However, in that it relies on highly viscous thermoplastic resists, it is limited in its ability to imprint precise patterns at a high speed. Moreover, the concentrated imprint force applied in R2RIL can damage the resist material which is structurally vulnerable at high process temperatures. Therefore, it is important to understand the temperature- and time-dependent characteristics of the resist material as well as the imprinting mechanism when using thermal R2RIL. In this work, the effects of the process temperature and rolling speed on thermal R2RIL of polycarbonate (PC) films were investigated to improve the process efficiency. Micro-scale line patterns were successfully transferred onto PC films from nickel (Ni) mold stamps. Consequently, line patterns with widths in the range of 5–80 µm were achieved at a traveling speed of 28.6 mm s –1 and process temperature of 150 °C, which is just above the glass transition temperature (T g ). In addition, the patterning performance was investigated for different temperatures, rolling speeds and pattern sizes. The imprinted pattern profiles were measured by an alpha-step surface profiler to investigate the patterning performance. The results show that a much better imprint performance was achieved at 150 °C, compared to the result at temperatures below T g . The physical mechanisms of thermal R2RIL on a PC film were studied by a finite-element analysis and the patterning process was successfully demonstrated by a visco-plastic deformation model. (paper)

Type - rolle - formål

DEFF Research Database (Denmark)

Hermansen, Judy

2008-01-01

kategoriseringer af brands, som er nødvendige for en strategisk styring af samspillet. Hans brand taksonomi er en systematisk tilgang, som gør det muligt, at klassificere alle de brandede elementer i et tilbud, uanset om de er repræsenteret af ét brand alene eller er en kombination af to eller flere brands....... Taksonomien redegør for de forskellige strategiske roller og formål, som hvert enkelt brand element kan spille. Udgivelsesdato: maj......I dag handler brand building mere om at få mest muligt ud af de brands, der allerede er på markedet, end om at launche nye brands. For at få brands til at spille sammen kræver det organisering af deres indbyrdes relationer. Rob Oslers brand taksonomier er et redskab til at foretage de analyser og...

The effect of rolling draughts on texture and microstructure in aluminium

DEFF Research Database (Denmark)

Mishin, Oleg; Juul Jensen, D.; Bay, B.

1999-01-01

The texture gradients and microstructural variations are investigated in commercially pure aluminium plates 40% cold-rolled either with small draughts or with intermediate draughts. In these two samples, different textures are observed near the quarter thickness layer. A pronounced shear texture...... is found in the sample rolled with small draughts, while a rolling texture is observed in the sample rolled with intermediate draughts. Also, significant differences were found in the rolled microstrucrures near the quarter thickness. After rolling with intermediate draughts, extended dislocation...... boundaries preferentially aligned at an angle of about 25-45o to the rolling direction are observed in the longitudinal section. After rolling with small draughts, extended dislocation boundaries are preferentially aligned closer to the rolling direction. These results are discribed and discussed....

Roll-to-roll manufacturing of amorphous silicon alloy solar cells with in situ cell performance diagnostics

International Nuclear Information System (INIS)

Izu, M.; Ellison, T.

2003-01-01

In order to meet the price target necessary for widespread use of solar cell products, Energy Conversion Devices, Inc., ECD, has developed and commercialized a continuous roll-to-roll manufacturing technology for the production of a-Si alloy solar cells. Since the early 1980s, we have advanced this technology from a small-scale pilot machine to a large-scale production machine. In 2002, ECD commissioned a 30 MW per year machine for United Solar Systems Corp. in Auburn Hills, Michigan. The RF PECVD a-Si alloy solar cell processor, designed and built by ECD, deposits triple-junction solar cell materials consisting of nine layers of a-Si alloys in a continuous roll-to-roll operation simultaneously on six coils of 130 μm thick, 0.36 m wide, 2.6 km long stainless-steel substrate at 1 cm/s. In order to minimize production losses due to undetected deviations of production conditions and carry on a continuous program of device optimization, we have developed and are incorporating in situ cell performance diagnostic systems. (author)

In vivo siRNA delivery system for targeting to the liver by poly-l-glutamic acid-coated lipoplex

Directory of Open Access Journals (Sweden)

Yoshiyuki Hattori

2014-01-01

Full Text Available In this study, we developed anionic polymer-coated liposome/siRNA complexes (lipoplexes with chondroitin sulfate C (CS, poly-l-glutamic acid (PGA and poly-aspartic acid (PAA for siRNA delivery by intravenous injection, and evaluated the biodistribution and gene silencing effect in mice. The sizes of CS-, PGA- and PAA-coated lipoplexes were about 200 nm and their ζ-potentials were negative. CS-, PGA- and PAA-coated lipoplexes did not induce agglutination after mixing with erythrocytes. In terms of biodistribution, siRNAs after intravenous administration of cationic lipoplexes were largely observed in the lungs, but those of CS-, PGA- and PAA-coated lipoplexes were in both the liver and the kidneys, indicating that siRNA might be partially released from the anionic polymer-coated lipoplexes in the blood circulation and accumulate in the kidney, although the lipoplexes can prevent the agglutination with blood components. To increase the association between siRNA and cationic liposome, we used cholesterol-modified siRNA (siRNA-Chol for preparation of the lipoplexes. When CS-, PGA- and PAA-coated lipoplexes of siRNA-Chol were injected into mice, siRNA-Chol was mainly observed in the liver, not in the kidneys. In terms of the suppression of gene expression in vivo, apolipoprotein B (ApoB mRNA in the liver was significantly reduced 48 h after single intravenous injection of PGA-coated lipoplex of ApoB siRNA-Chol (2.5 mg siRNA/kg, but not cationic, CS- and PAA-coated lipoplexes. In terms of toxicity after intravenous injection, CS-, PGA- and PAA-coated lipoplexes did not increase GOT and GPT concentrations in blood. From these findings, PGA coatings for cationic lipoplex of siRNA-Chol might produce a systemic vector of siRNA to the liver.

ROLL OUT THE TALENT : Final project report

OpenAIRE

Eerola, Tuomas; Tuominen, Pirjo; Hakkarainen, Riitta-Liisa; Laurikainen, Marja; Mero, Niina

2014-01-01

The ROLL OUT THE TALENT project was born out of the desire to recognise and support the strengths of vocational students and to develop new and innovative operating models. ROLL OUT THE TALENT promoted regional cooperation between institutes and companies. The project produced operating and study path models that take into consideration the individual strengths of vocational students and the principles of lifelong learning. This is the final report of the ROLL OUT THE TALENT project, and ...

Effect of Reduction in Thickness and Rolling Conditions on Mechanical Properties and Microstructure of Rolled Mg-8Al-1Zn-1Ca Alloy

Directory of Open Access Journals (Sweden)

Yuta Fukuda

2017-01-01

Full Text Available A cast Mg-8Al-1Zn-1Ca magnesium alloy was multipass hot rolled at different sample and roll temperatures. The effect of the rolling conditions and reduction in thickness on the microstructure and mechanical properties was investigated. The optimal combination of the ultimate tensile strength, 351 MPa, yield strength, 304 MPa, and ductility, 12.2%, was obtained with the 3 mm thick Mg-8Al-1Zn-1Ca rolled sheet, which was produced with a roll temperature of 80°C and sample temperature of 430°C. This rolling process resulted in the formation of a bimodal structure in the α-Mg matrix, which consequently led to good ductility and high strength, exclusively by the hot rolling process. The 3 mm thick rolled sheet exhibited fine (mean grain size of 2.7 μm and coarse grain regions (mean grain size of 13.6 μm with area fractions of 29% and 71%, respectively. In summary, the balance between the strength and ductility was enhanced by the grain refinement of the α-Mg matrix and by controlling the frequency and orientation of the grains.

Evolution of RLSB, a nuclear-encoded S1 domain RNA binding protein associated with post-transcriptional regulation of plastid-encoded rbcL mRNA in vascular plants.

Science.gov (United States)

Yerramsetty, Pradeep; Stata, Matt; Siford, Rebecca; Sage, Tammy L; Sage, Rowan F; Wong, Gane Ka-Shu; Albert, Victor A; Berry, James O

2016-06-29

RLSB, an S-1 domain RNA binding protein of Arabidopsis, selectively binds rbcL mRNA and co-localizes with Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) within chloroplasts of C3 and C4 plants. Previous studies using both Arabidopsis (C3) and maize (C4) suggest RLSB homologs are post-transcriptional regulators of plastid-encoded rbcL mRNA. While RLSB accumulates in all Arabidopsis leaf chlorenchyma cells, in C4 leaves RLSB-like proteins accumulate only within Rubisco-containing bundle sheath chloroplasts of Kranz-type species, and only within central compartment chloroplasts in the single cell C4 plant Bienertia. Our recent evidence implicates this mRNA binding protein as a primary determinant of rbcL expression, cellular localization/compartmentalization, and photosynthetic function in all multicellular green plants. This study addresses the hypothesis that RLSB is a highly conserved Rubisco regulatory factor that occurs in the chloroplasts all higher plants. Phylogenetic analysis has identified RLSB orthologs and paralogs in all major plant groups, from ancient liverworts to recent angiosperms. RLSB homologs were also identified in algae of the division Charophyta, a lineage closely related to land plants. RLSB-like sequences were not identified in any other algae, suggesting that it may be specific to the evolutionary line leading to land plants. The RLSB family occurs in single copy across most angiosperms, although a few species with two copies were identified, seemingly randomly distributed throughout the various taxa, although perhaps correlating in some cases with known ancient whole genome duplications. Monocots of the order Poales (Poaceae and Cyperaceae) were found to contain two copies, designated here as RLSB-a and RLSB-b, with only RLSB-a implicated in the regulation of rbcL across the maize developmental gradient. Analysis of microsynteny in angiosperms revealed high levels of conservation across eudicot species and for both paralogs in

Plastic anisotropy of straight and cross rolled molybdenum sheets

International Nuclear Information System (INIS)

Oertel, C.-G.; Huensche, I.; Skrotzki, W.; Knabl, W.; Lorich, A.; Resch, J.

2008-01-01

The microstructure, texture and mechanical properties of molybdenum sheets produced by different rolling processes were investigated by orientation imaging in the scanning electron microscope, X-ray diffraction and tensile tests, respectively. For comparable recrystallization degree of the sheets investigated, straight rolling with low reduction ratio produces α-fiber textures with a maximum at {100} . At higher rolling degrees the maximum shifts to {112} . Cross rolling increases the rotated cube component {100} . The strong differences in the texture measured are reflected in the plastic anisotropy characterized by differences in the yield stress and Lankford parameter which were measured along directions in the rolling plane at angles of 0 deg., 45 deg. and 90 deg. with the rolling direction. The Taylor-Bishop-Hill theory is used successfully to qualitatively explain the plastic anisotropy

Maintenance of the marginal zone B cell compartment specifically requires the RNA-binding protein ZFP36L1

Science.gov (United States)

Newman, Rebecca; Ahlfors, Helena; Saveliev, Alexander; Galloway, Alison; Hodson, Daniel J; Williams, Robert; Besra, Gurdyal S.; Cook, Charlotte N; Cunningham, Adam F; Bell, Sarah E; Turner, Martin

2017-01-01

RNA binding proteins (RBP) of the ZFP36 family are best known for inhibiting the expression of cytokines through binding to AU rich elements in the 3’UTR and promoting mRNA decay. Here we show an indispensible role for ZFP36L1 as the regulator of a post-transcriptional hub that determined the identity of marginal zone (MZ) B cells by promoting their proper localization and survival. ZFP36L1 controlled a gene expression program related to signaling, cell-adhesion and locomotion, in part by limiting the expression of the transcription factors KLF2 and IRF8, which are known to enforce the follicular B cell phenotype. These mechanisms emphasize the importance of integrating transcriptional and post-transcriptional processes by RBP for maintaining cellular identity between closely related cell types. PMID:28394372

Structures of a putative RNA 5-methyluridine methyltransferase, Thermus thermophilus TTHA1280, and its complex with S-adenosyl-l-homocysteine

International Nuclear Information System (INIS)

Pioszak, Augen A.; Murayama, Kazutaka; Nakagawa, Noriko; Ebihara, Akio; Kuramitsu, Seiki; Shirouzu, Mikako; Yokoyama, Shigeyuki

2005-01-01

Three structures of a putative RNA 5-methyluridine methyltransferase from T. thermophilus, including its complex with S-adenosyl-l-homocysteine, are presented. The structures reveal the mode of cofactor binding, architecture of the putative active site, and the presence of a deep cleft adjacent to the active site that may bind RNA. The Thermus thermophilus hypothetical protein TTHA1280 belongs to a family of predicted S-adenosyl-l-methionine (AdoMet) dependent RNA methyltransferases (MTases) present in many bacterial and archaeal species. Inspection of amino-acid sequence motifs common to class I Rossmann-fold-like MTases suggested a specific role as an RNA 5-methyluridine MTase. Selenomethionine (SeMet) labelled and native versions of the protein were expressed, purified and crystallized. Two crystal forms of the SeMet-labelled apoprotein were obtained: SeMet-ApoI and SeMet-ApoII. Cocrystallization of the native protein with S-adenosyl-l-homocysteine (AdoHcy) yielded a third crystal form, Native-AdoHcy. The SeMet-ApoI structure was solved by the multiple anomalous dispersion method and refined at 2.55 Å resolution. The SeMet-ApoII and Native-AdoHcy structures were solved by molecular replacement and refined at 1.80 and 2.60 Å, respectively. TTHA1280 formed a homodimer in the crystals and in solution. Each subunit folds into a three-domain structure composed of a small N-terminal PUA domain, a central α/β-domain and a C-terminal Rossmann-fold-like MTase domain. The three domains form an overall clamp-like shape, with the putative active site facing a deep cleft. The architecture of the active site is consistent with specific recognition of uridine and catalysis of methyl transfer to the 5-carbon position. The cleft is suitable in size and charge distribution for binding single-stranded RNA.

Tachyon constant-roll inflation

Science.gov (United States)

Mohammadi, A.; Saaidi, Kh.; Golanbari, T.

2018-04-01

The constant-roll inflation is studied where the inflaton is taken as a tachyon field. Based on this approach, the second slow-roll parameter is taken as a constant which leads to a differential equation for the Hubble parameter. Finding an exact solution for the Hubble parameter is difficult and leads us to a numerical solution for the Hubble parameter. On the other hand, since in this formalism the slow-roll parameter η is constant and could not be assumed to be necessarily small, the perturbation parameters should be reconsidered again which, in turn, results in new terms appearing in the amplitude of scalar perturbations and the scalar spectral index. Utilizing the numerical solution for the Hubble parameter, we estimate the perturbation parameter at the horizon exit time and compare it with observational data. The results show that, for specific values of the constant parameter η , we could have an almost scale-invariant amplitude of scalar perturbations. Finally, the attractor behavior for the solution of the model is presented, and we determine that the feature could be properly satisfied.

Kilplased saavad eesti filmi juubeliaastaks kilbi läikima / Erik Müürsepp

Index Scriptorium Estoniae

Müürsepp, Erik

2010-01-01

Tallinnfilm plaanib 2010. aasta jooksul juubeliprogrammi "Eesti film 100" raames digiteerida ning taastada pildi ja heli kolmel Rein Raamatu joonisfilmil: "Kilplased" (1974), "Suur Tõll" (1980) ja "Põrgu" (1983)

Axial Force Analysis and Roll Contour Configuration of Four-High CVC Mill

Directory of Open Access Journals (Sweden)

Guang-ming Liu

2018-01-01

Full Text Available In order to analyze the influence of technical parameters on work roll axial force of four-high continuous variable crown (CVC mill, the deformation analyzing model with top roll system and strip was established based on influence function method. Then a CVC work roll curve designing scheme was proposed and it was carried out on some cold rolling mill considering the requirement of comprehensive work roll axial force minimization. The status of comprehensive work roll axial force is improved considering the rolling schedule that is beneficial to the roller bearing. Corresponding to the newly designed work roll contour, the backup roll end chamfer was designed considering comprehensive performance of interroll stress concentration, comprehensive work roll axial force, and strip shape control ability. The distribution of roll wear with newly designed backup roll contour is more even according to the field application data. The newly established roll configuration scheme is beneficial to four-high CVC mill.

Sirbi tegijad läbi aegade

Index Scriptorium Estoniae

2004-01-01

Toimetaja asetäitjad: Katrin Kivimaa, Reet Varblane jt. Kunst, arhitektuur: Alo Hoidre, Boris Lukats, Ressi Kaera, Jaak Olep, Ike Volkov, Mirjam Peil, Tiina Käesel, Katrin Kivimaa, Vappu Vabar, Reet Varblane, Mari Laaniste. Kunstnikud (arvutikujundus): Tõnu Soo, Ann Gornischeff, Gädvi Kuklase, Piia Ruber. Fotograafid: Solomon Rosenfeldt, Endel Köster, Kalju Suur, Arno Saar, Teet Malsroos

All polymer photovoltaics: From small inverted devices to large roll-to-roll coated and printed solar cells

DEFF Research Database (Denmark)

Liu, Yao; Larsen-Olsen, Thue Trofod; Zhao, Xingang

2013-01-01

Inverted all polymer solar cells based on a blend of a perylene diimide based polymer acceptor and a dithienosilole based polymer donor were fabricated from small area devices to roll-to-roll (R2R) coated and printed large area modules. The device performance was successfully optimized by using...

Measurement of Normal and Friction Forces in a Rolling Process

DEFF Research Database (Denmark)

Henningsen, Poul; Arentoft, Mogens; Wanheim, Tarras

2004-01-01

by the fric-tion conditions. To achieve this important informa-tion, measurements of the normal pressure and friction stresses in the deformation zone are re-quested. The direction of the friction stresses is changing during the rolling gap. At the entrance of the de-formation zone, the peripherical velocity...... of the roll is higher than for the incoming material, which causes frictional stresses at the material acting in the rolling direction. At the outlet of the rolling gap, the velocity of the deformed material exceeds the velocity of the roll, generating frictional stresses contrary to the direction of rolling....... In a narrow area in the deformation zone, the velocity of the de-formed material is equal to the velocity of the rolls. This area or line is named “neutral line”. The posi-tion of the neutral line depends on friction, reduc-tion ratio, diameter of the rolls, and width of the sheet....

Parametric roll resonance monitoring using signal-based detection

DEFF Research Database (Denmark)

Galeazzi, Roberto; Blanke, Mogens; Falkenberg, Thomas

2015-01-01

Extreme roll motion of ships can be caused by several phenomena, one of which is parametric roll resonance. Several incidents occurred unexpectedly around the millennium and caused vast fiscal losses on large container vessels. The phenomenon is now well understood and some consider parametric roll...... algorithms in real conditions, and to evaluate the frequency of parametric roll events on the selected vessels. Detection performance is scrutinised through the validation of the detected events using owners’ standard methods, and supported by available wave radar data. Further, a bivariate statistical...... analysis of the outcome of the signal-based detectors is performed to assess the real life false alarm probability. It is shown that detection robustness and very low false warning rates are obtained. The study concludes that small parametric roll events are occurring, and that the proposed signal...

Spring-back of flexible roll forming bending process

International Nuclear Information System (INIS)

Zhang, Y; Kim, D H; Jung, D W

2015-01-01

Simulations are now widely used in the field of roll forming because of their convenience. Simulations provide a low cost, secure and fast analysis tool. Flexible roll forming provides the desired shapes with a one time forming process. For roll forming, the velocity of the sheet and friction are important factors to attain an ideal shape. Because it is a complicated process, simulations provide a better understanding of the roll forming process. Simulations were peformed using ABAQUS software linked to elastic-plastic modules which we developed taking into account of interactions between these fields [1]. The application of this method makes it possible to highlight the strain-stress and mechanical behaviour laws and the spring-back. Thus, the flexible roll forming and bending process can be well described by the simulation software and guide the actual machine. (paper)

Transient thermal stresses of work roll by coupled thermoelasticity

Science.gov (United States)

Lai, W. B.; Chen, T. C.; Weng, C. I.

1991-01-01

A numerical method, based on a two-dimensional plane strain model, is developed to predict the transient responses (that include distributions of temperature, thermal deformation, and thermal stress) of work roll during strip rolling by coupled thermoelasticity. The method consists of discretizing the space domain of the problem by finite element method first, and then treating the time domain by implicit time integration techniques. In order to avoid the difficulty in analysis due to relative movement between work roll and its thermal boundary, the energy equation is formulated with respect to a fixed Eulerian reference frame. The effect of thermoelastic coupling term, that is generally disregarded in strip rolling, can be considered and assessed. The influences of some important process parameters, such as rotational speed of the roll and intensity of heat flux, on transient solutions are also included and discussed. Furthermore, since the stress history at any point of the roll in both transient and steady state could be accurately evaluated, it is available to perform the analysis of thermal fatigue for the roll by means of previous data.

Quality control of roll-to-roll processed polymer solar modules by complementary imaging methods

DEFF Research Database (Denmark)

Rösch, R.; Krebs, Frederik C; Tanenbaum, D.M.

2012-01-01

We applied complementary imaging methods to investigate processing failures of roll-to-roll solution processed polymer solar modules based on polymer:fullerene bulk heterojunctions. For investigation of processing deficiencies in solar modules we employed dark lock-in thermography (DLIT......), electroluminescence (ELI) and photoluminescence/reflection imaging (PLI/RI) complemented by optical imaging (OI). The combination of all high resolution images allowed us to allocate the origin of processing errors to a specific deposition process, i.e. the insufficient coverage of an electrode interlayer...

Hot rolling and annealing effects on the microstructure and mechanical properties of ODS austenitic steel fabricated by electron beam selective melting

Science.gov (United States)

Gao, Rui; Ge, Wen-jun; Miao, Shu; Zhang, Tao; Wang, Xian-ping; Fang, Qian-feng

2016-03-01

The grain morphology, nano-oxide particles and mechanical properties of oxide dispersion strengthened (ODS)-316L austenitic steel synthesized by electron beam selective melting (EBSM) technique with different post-working processes, were explored in this study. The ODS-316L austenitic steel with superfine nano-sized oxide particles of 30-40 nm exhibits good tensile strength (412 MPa) and large total elongation (about 51%) due to the pinning effect of uniform distributed oxide particles on dislocations. After hot rolling, the specimen exhibits a higher tensile strength of 482 MPa, but the elongation decreases to 31.8% owing to the introduction of high-density dislocations. The subsequent heat treatment eliminates the grain defects induced by hot rolling and increases the randomly orientated grains, which further improves the strength and ductility of EBSM ODS-316L steel.

METHOD OF ROLLING URANIUM

Science.gov (United States)

Smith, C.S.

1959-08-01

A method is described for rolling uranium metal at relatively low temperatures and under non-oxidizing conditions. The method involves the steps of heating the uranium to 200 deg C in an oil bath, withdrawing the uranium and permitting the oil to drain so that only a thin protective coating remains and rolling the oil coated uranium at a temperature of 200 deg C to give about a 15% reduction in thickness at each pass. The operation may be repeated to accomplish about a 90% reduction without edge cracking, checking or any appreciable increase in brittleness.

Chaotic travelling rolls in Rayleigh–Bénard convection

Indian Academy of Sciences (India)

The lateral shift of the rolls may lead to a global flow reversal of the convective motion. The chaotic travelling rolls are observed in simulations with free-slip as well as no-slip boundary conditions on the velocity field. We show that the travelling rolls and the flow reversal are due to an interplay between the real and imaginary ...

Experimental and theoretical study on minimum achievable foil thickness during asymmetric rolling.

Directory of Open Access Journals (Sweden)

Delin Tang

Full Text Available Parts produced by microforming are becoming ever smaller. Similarly, the foils required in micro-machines are becoming ever thinner. The asymmetric rolling technique is capable of producing foils that are thinner than those produced by the conventional rolling technique. The difference between asymmetric rolling and conventional rolling is the 'cross-shear' zone. However, the influence of the cross-shear zone on the minimum achievable foil thickness during asymmetric rolling is still uncertain. In this paper, we report experiments designed to understand this critical influencing factor on the minimum achievable thickness in asymmetric rolling. Results showed that the minimum achievable thickness of rolled foils produced by asymmetric rolling with a rolling speed ratio of 1.3 can be reduced to about 30% of that possible by conventional rolling technique. Furthermore, the minimum achievable thickness during asymmetric rolling could be correlated to the cross-shear ratio, which, in turn, could be related to the rolling speed ratio. From the experimental results, a formula to calculate the minimum achievable thickness was established, considering the parameters cross-shear ratio, friction coefficient, work roll radius, etc. in asymmetric rolling.

Experimental and theoretical study on minimum achievable foil thickness during asymmetric rolling.

Science.gov (United States)

Tang, Delin; Liu, Xianghua; Song, Meng; Yu, Hailiang

2014-01-01

Parts produced by microforming are becoming ever smaller. Similarly, the foils required in micro-machines are becoming ever thinner. The asymmetric rolling technique is capable of producing foils that are thinner than those produced by the conventional rolling technique. The difference between asymmetric rolling and conventional rolling is the 'cross-shear' zone. However, the influence of the cross-shear zone on the minimum achievable foil thickness during asymmetric rolling is still uncertain. In this paper, we report experiments designed to understand this critical influencing factor on the minimum achievable thickness in asymmetric rolling. Results showed that the minimum achievable thickness of rolled foils produced by asymmetric rolling with a rolling speed ratio of 1.3 can be reduced to about 30% of that possible by conventional rolling technique. Furthermore, the minimum achievable thickness during asymmetric rolling could be correlated to the cross-shear ratio, which, in turn, could be related to the rolling speed ratio. From the experimental results, a formula to calculate the minimum achievable thickness was established, considering the parameters cross-shear ratio, friction coefficient, work roll radius, etc. in asymmetric rolling.

WTO läbimurde ootel / Tõnis Arnover

Index Scriptorium Estoniae

Arnover, Tõnis, 1952-

2006-01-01

Maailma Kaubandusorganisatsioonis oodatakse Suure Leppe sündi, mis oleks kasulik ekspordist huvitatud avatud majandusega riikidele. Vt. samas: Tähtsamad huvigrupid WTO-s; Šveitsi valem kärbib kõrgeid tariife; Mauritius pelgab oma kaupade konkurentsivõime kahanemist; Eestit huvitavad tööstuskaupade eksportimise lisavõimalused; Mina sulle, sina mulle

RNA-Binding Protein L1TD1 Interacts with LIN28 via RNA and is Required for Human Embryonic Stem Cell Self-Renewal and Cancer Cell Proliferation

OpenAIRE

Närvä, Elisa; Rahkonen, Nelly; Emani, Maheswara Reddy; Lund, Riikka; Pursiheimo, Huha-Pekka; Nästi, Juuso; Autio, Reija; Rasool, Omid; Denessiouk, Konstantin; Lähdesmäki, Harri; Rao, Anjana; Lahesmaa, Ritta

2012-01-01

Human embryonic stem cells (hESC) have a unique capacity to self-renew and differentiate into all the cell types found in human body. Although the transcriptional regulators of pluripotency are well studied, the role of cytoplasmic regulators is still poorly characterized. Here, we report a new stem cell-specific RNA-binding protein L1TD1 (ECAT11, FLJ10884) required for hESC self-renewal and cancer cell proliferation. Depletion of L1TD1 results in immediate downregulation of OCT4 and NANOG. F...

Voltage and Thermally Driven Roll-to-Roll Organic Printed Transistor Made in Ambient Air Conditions

DEFF Research Database (Denmark)

Pastorelli, Francesco

of the organic semiconductor poly3hexylthiophene and the dielectric material polyvinylphenol before the gate was applied by screen printing. All the processing was realized in ambient air on a PET flexible substrate. We explore the footprint and the practically accessible geometry of such devices with a special......Resume: Organic thin film transistors offer great potential for use in flexible electronics. Much of this potential lies in the solution processability of the organic polymers enabling both roll coating and printing on flexible substrates and thus greatly reducing the material and fabrication costs....... We present flexible organic power transistors prepared by fast (20 m min−1) roll-to-roll flexographic printing of the drain and source electrode structures, with an interspace below 50 um, directly on polyester foil[1]. The devices have top gate architecture and were completed by slotdie coating...

Continuous roll-to-roll a-Si photovoltaic manufacturing technology. Annual subcontractor report, 1 April 1992--31 March 1993

Energy Technology Data Exchange (ETDEWEB)

Izu, M. [Energy Conversion Devices, Inc., Troy, MI (United States)

1993-12-01

This report describes work done under a 3-year program to advance ECD`s roll-to-roll, triple-junction photovoltaic manufacturing technologies, to reduce the module production costs, to increase the stabilized module performance, and to expand commercial capacity utilizing ECD technology. The specific 3-year goal is to develop advanced large-scale manufacturing technology incorporating ECD`s earlier research advances with the capability of producing modules with stable 11% efficiency at a cost of approximately $1.00 per peak watt. Accomplishments during Phase 1 included: (1) ECD successfully incorporated a high-performance Ag/metal-oxide back-reflector system into its continuous roll-to-roll commercial production operation. (2) High-quality a-Si-Ge narrow-band-gap solar cells were incorporated into the manufacturing. (3) ECD demonstrated the continuous roll-to-roll production of high-efficiency, triple-junction, two-band-gap solar cells consistently and uniformly throughout a 762-m (2500-ft) run with high yield. (4) ECD achieved 11.1% initial sub-cell efficiency of triple-junction, two-band-gap a-Si alloy solar cells in the production line. (5) The world`s first 0.37-m{sup 2} (4-ft{sup 2}) PV modules were produced utilizing triple-junction spectrum-splitting solar cells manufactured in the production line. (6) As a result of process optimization to reduce the layer thickness and to improve the gas utilization, ECD achieved a 77% material cost reduction for germane and 58% reduction for disilane. Additionally, ECD developed a new low-cost module that saves approximately 30% in assembly material costs.

Ajalehtede lõpp / Abdul Turay

Index Scriptorium Estoniae

Turay, Abdul, 1967-

2011-01-01

1843. aastast ilmunud Briti tabloidi News of the World suleti erakõnede pealtkuulamise tõttu. News of the World'i peadirektorit Rebekah Brooksi ja meediaparun Rupert Murdochi kardavad isegi poliitikud, kes ei julge neid avalikult kritiseerida. Ajalehti juhib kitsas ring inimesi ja neil on liiga suur võim käes. Eestis valitseb teistsugune olekord - ükski poliitik ei karda tõsiselt ühtegi ajakirjanikku

A long and abundant non-coding RNA in Lactobacillus salivarius.

Science.gov (United States)

Cousin, Fabien J; Lynch, Denise B; Chuat, Victoria; Bourin, Maxence J B; Casey, Pat G; Dalmasso, Marion; Harris, Hugh M B; McCann, Angela; O'Toole, Paul W

2017-09-01

Lactobacillus salivarius , found in the intestinal microbiota of humans and animals, is studied as an example of the sub-dominant intestinal commensals that may impart benefits upon their host. Strains typically harbour at least one megaplasmid that encodes functions contributing to contingency metabolism and environmental adaptation. RNA sequencing (RNA-seq)transcriptomic analysis of L. salivarius strain UCC118 identified the presence of a novel unusually abundant long non-coding RNA (lncRNA) encoded by the megaplasmid, and which represented more than 75 % of the total RNA-seq reads after depletion of rRNA species. The expression level of this 520 nt lncRNA in L. salivarius UCC118 exceeded that of the 16S rRNA, it accumulated during growth, was very stable over time and was also expressed during intestinal transit in a mouse. This lncRNA sequence is specific to the L. salivarius species; however, among 45 L . salivarius genomes analysed, not all (only 34) harboured the sequence for the lncRNA. This lncRNA was produced in 27 tested L. salivarius strains, but at strain-specific expression levels. High-level lncRNA expression correlated with high megaplasmid copy number. Transcriptome analysis of a deletion mutant lacking this lncRNA identified altered expression levels of genes in a number of pathways, but a definitive function of this new lncRNA was not identified. This lncRNA presents distinctive and unique properties, and suggests potential basic and applied scientific developments of this phenomenon.

Development of a Minimally Actuated Jumping-Rolling Robot

Directory of Open Access Journals (Sweden)

Thanhtam Ho

2015-04-01

Full Text Available This paper presents development of a hybrid mobile robot in order to take advantage of both rolling and jumping locomotion on the ground. According to the unique design of the mechanism, the robot is able to execute both jumping and rolling skilfully by using only one DC motor. Changing the centre of gravity enables rolling of the robot and storage of energy is utilized for jumping. Mechanism design and control logic are validated by computer simulation. Simulation results show that the robot can jump nearly 1.3 times its diameter and roll at the speed of 3.3 times its diameter per second.

Veneetsia filmifestival lõppes Iraani võiduga / Tiit Tuumalu

Index Scriptorium Estoniae

Tuumalu, Tiit, 1971-

2000-01-01

Kuldlõvi sai Jafar Panahi mängufilm "Ring" ("Dayereh"), žürii suure preemia USA režissööri Julian Schnabeli "Enne kui öö saabub" ("Before Night Falls"), parima stsenaariumi preemia itaallase Marco Tullio Giordana "Sada sammu" ("I cento passi")

Adaptive attenuation of aliased ground roll using the shearlet transform

Science.gov (United States)

Hosseini, Seyed Abolfazl; Javaherian, Abdolrahim; Hassani, Hossien; Torabi, Siyavash; Sadri, Maryam

2015-01-01

Attenuation of ground roll is an essential step in seismic data processing. Spatial aliasing of the ground roll may cause the overlap of the ground roll with reflections in the f-k domain. The shearlet transform is a directional and multidimensional transform that separates the events with different dips and generates subimages in different scales and directions. In this study, the shearlet transform was used adaptively to attenuate aliased and non-aliased ground roll. After defining a filtering zone, an input shot record is divided into segments. Each segment overlaps adjacent segments. To apply the shearlet transform on each segment, the subimages containing aliased and non-aliased ground roll, the locations of these events on each subimage are selected adaptively. Based on these locations, mute is applied on the selected subimages. The filtered segments are merged together, using the Hanning function, after applying the inverse shearlet transform. This adaptive process of ground roll attenuation was tested on synthetic data, and field shot records from west of Iran. Analysis of the results using the f-k spectra revealed that the non-aliased and most of the aliased ground roll were attenuated using the proposed adaptive attenuation procedure. Also, we applied this method on shot records of a 2D land survey, and the data sets before and after ground roll attenuation were stacked and compared. The stacked section after ground roll attenuation contained less linear ground roll noise and more continuous reflections in comparison with the stacked section before the ground roll attenuation. The proposed method has some drawbacks such as more run time in comparison with traditional methods such as f-k filtering and reduced performance when the dip and frequency content of aliased ground roll are the same as those of the reflections.

Rolling process simulation of a pair-crossed hot strip mill

International Nuclear Information System (INIS)

Chen Shaojie; Xu Jianzhong; Liu Xianghua; Wang Guodong

2000-01-01

Process simulation can help optimize the operating parameters aiming to improve the quality of rolled products. In this paper, software in Visual Basic language is developed to simulate the hot rolling process of a pair-crossed mill. The strip temperature is calculated by considering air cooling, water cooling, heat generation and conduction.The production parameters including rolling speeds, resistance to deformation, rolling forces, drive torques and powers are evaluated by mathematical models and their parameter identification support tools. The deformation of roll stack is calculated by influential function method. The roll temperature and expansion are calculated by finite differential method, and the roll wear is described by empirical formula. Based on these calculations as well as the effect of heredity is taken into account, the strip crown and flatness then can be obtained. The results show that the simulation software has friendly user interface, high accuracy and practicability. It can be served as a basis for the mill design and optimization of process parameters to acquire high quality of hot rolled strip. (author)

Decision Support for the Rolling Stock Dispatcher

DEFF Research Database (Denmark)

Groth, Julie Jespersen

Real-time recovery is receiving a fast growing interest in an increasingly competitive railway operation market. This thesis considers the area of rolling stock dispatching which is one of the typical real-time railway dispatching problems. All work of the thesis is based on the network...... and planning processes of the railway operator DSB S-tog a/s. In the thesis the problems existing in the railway planning process from the strategic to real-time level are briefly sketched. Network planning, line planning, timetabling, crew and rolling stock planning is outlined and relevant references...... are given. Specifically the thesis references the operation research studies based on the railway operation of DSB S-tog a/s. Subsequently the process of dispatching is outlined with a specific emphasis on rolling stock. The rolling stock recovery problem is the problem of assigning train units to train...

Numerical analysis of Swiss roll metamaterials

International Nuclear Information System (INIS)

Demetriadou, A; Pendry, J B

2009-01-01

A Swiss roll metamaterial is a resonant magnetic medium, with a negative magnetic permeability for a range of frequencies, due to its self-inductance and self-capacitance components. In this paper, we discuss the band structure, S-parameters and effective electromagnetic parameters of Swiss roll metamaterials, with both analytical and numerical results, which show an exceptional convergence.

Vanemuise suure maja fuajees...

Index Scriptorium Estoniae

2005-01-01

6. IX avati näitleja ja lavastaja Evald Hermaküla mälestuseks fotonäitus, mille on koostanud ja kujundanud Tio Tepandi. Näitus hõlmab E. Hermaküla kümmet noorusaastat 1960-ndate lõpust ja 1970-ndate algusest. Mais oli näitus üleval Tallinnas Eesti Draamateatris

The nucleotide sequence of RNA1 of Lettuce big-vein virus, genus Varicosavirus, reveals its relation to nonsegmented negative-strand RNA viruses.

Science.gov (United States)

Sasaya, Takahide; Ishikawa, Koichi; Koganezawa, Hiroki

2002-06-05

The complete nucleotide sequence of RNA1 from Lettuce big-vein virus (LBVV), the type member of the genus Varicosavirus, was determined. LBVV RNA1 consists of 6797 nucleotides and contains one large ORF that encodes a large (L) protein of 2040 amino acids with a predicted M(r) of 232,092. Northern blot hybridization analysis indicated that the LBVV RNA1 is a negative-sense RNA. Database searches showed that the amino acid sequence of L protein is homologous to those of L polymerases of nonsegmented negative-strand RNA viruses. A cluster dendrogram derived from alignments of the LBVV L protein and the L polymerases indicated that the L protein is most closely related to the L polymerases of plant rhabdoviruses. Transcription termination/polyadenylation signal-like poly(U) tracts that resemble those in rhabdovirus and paramyxovirus RNAs were present upstream and downstream of the coding region. Although LBVV is related to rhabdoviruses, a key distinguishing feature is that the genome of LBVV is segmented. The results reemphasize the need to reconsider the taxonomic position of varicosaviruses.

Refinement of the microstructure of steel by cross rolling

International Nuclear Information System (INIS)

Tsay, Kira; Arbuz, Alexandr; Gusseynov, Nazim; Nemkaeva, Renata; Ospanov, Nurlan; Krupen'kin, Ivan

2016-01-01

One of the most effective ways for refinement of metal microstructure is a severe plastic deformation. The cross rolling is the one of most perspective methods of severe plastic deformation, because it allows to get the long billets, unlike equal angular pressing and other popular methods. This fact provides some industrial expectation for this method. However, deformation and motion path of the metal is very heterogeneous across the section of the rolled piece. This paper presents the finite element modeling of hot cross rolling of steel in the software package DEFORM-3D features implemented and studied the stress-strain state. An experimental study of the effect of the cross rolling on a three-roll mill on the microstructure of structural alloy steel and stainless steel AISI321 in different zones of the bar. Analysis of microsections made after rolling with high total stretch and the final pass temperature 700°C, shows the formation of equiaxial ultrafinegrain structure on the periphery of an elongated rod and “rolling” texture in the central zone. The resulting microstructure corresponds to that obtained in models of stress-strain state. Keywords: cross rolling, ultra-fine grain structure, steel.

Helsingis avatakse suur Eesti maalinäitus

Index Scriptorium Estoniae

2010-01-01

Eesti maalikunsti ülevaatenäitus "Viron värit - 150 vuotta virolaista maalaustaideta Enn Kunilan kokoelamasta" ("Eesti värvid - 150 aastat eesti maalikunsti Enn Kunila kogust") Helsingi kunstihoones (Helsingin Taidehalli) 16. oktoobrist 24. novembrini 2010. Näituse kuraator Harry Liivrand, kujundaja Olev Subbi. Väljapanekut saatva raamatu on koostanud Eero Epner, kujundanud Tiit Jürna

Analysis of Residual Stress and Deformation of Rolling Strengthen Crankshaft Fillet

Directory of Open Access Journals (Sweden)

Han Shaojun

2016-01-01

Full Text Available Based on the analysis of crankshaft fillet rolling process, used ANSYS finite element analysis software to conduct the elastic-plastic mechanical simulation of crankshaft rolling process, and gained the variation law of the residual stress and plastic deformation in the radial path of the fillet under different rolling laps and rolling pressure. Established the relationship between the rolling pressure and the plastic deformation and residual stress of the fillet, and provided theoretical support for the evaluation and detection of the crankshaft rolling quality.

Continuous and scalable fabrication of bioinspired dry adhesives via a roll-to-roll process with modulated ultraviolet-curable resin.

Science.gov (United States)

Yi, Hoon; Hwang, Insol; Lee, Jeong Hyeon; Lee, Dael; Lim, Haneol; Tahk, Dongha; Sung, Minho; Bae, Won-Gyu; Choi, Se-Jin; Kwak, Moon Kyu; Jeong, Hoon Eui

2014-08-27

A simple yet scalable strategy for fabricating dry adhesives with mushroom-shaped micropillars is achieved by a combination of the roll-to-roll process and modulated UV-curable elastic poly(urethane acrylate) (e-PUA) resin. The e-PUA combines the major benefits of commercial PUA and poly(dimethylsiloxane) (PDMS). It not only can be cured within a few seconds like commercial PUA but also possesses good mechanical properties comparable to those of PDMS. A roll-type fabrication system equipped with a rollable mold and a UV exposure unit is also developed for the continuous process. By integrating the roll-to-roll process with the e-PUA, dry adhesives with spatulate tips in the form of a thin flexible film can be generated in a highly continuous and scalable manner. The fabricated dry adhesives with mushroom-shaped microstructures exhibit a strong pull-off strength of up to ∼38.7 N cm(-2) on the glass surface as well as high durability without any noticeable degradation. Furthermore, an automated substrate transportation system equipped with the dry adhesives can transport a 300 mm Si wafer over 10,000 repeating cycles with high accuracy.

A new transducer for local load measurements of friction and roll pressure in cold flat rolling

DEFF Research Database (Denmark)

Lagergren, J.; Wanheim, Tarras; Precz, W.

2006-01-01

The only way to establish the true rolling pressure and the true friction condition in cold rolling is to conduct measurements in the roll bite. A new transducer design is therefore proposed, in order to overcome problems in previous measurements in the past 70 years. The new idea is to increase ...... and able to avoid signal disturbance. The pressure and friction stress distribution results was as expected by the authors and showed good reproducibility, together with a proven agreement between recorded and simulated signals....... selected from a steady state with no disturbance from the material flow. The transducer was able to simultaneously measure both the normal pressure and the friction stress. An estimation of the coefficient of friction was accordingly performed. The new transducer works very well, it was seen to be robust...

Roll-to-roll printed resistive WORM memory on a flexible substrate

Science.gov (United States)

Leppäniemi, Jaakko; Mattila, Tomi; Kololuoma, Terho; Suhonen, Mika; Alastalo, Ari

2012-08-01

The fabrication process and the operation characteristics of a fully roll-to-roll printed resistive write-once-read-many memory on a flexible substrate are presented. The low-voltage (memories from a high resistivity ‘0’ state to a low resistivity ‘1’ state is based on the rapid electrical sintering of bits containing silver nanoparticles. The bit ink is formulated by mixing two commercially available silver nanoparticle inks in order to tune the initial square resistance of the bits and to create a self-organized network of percolating paths. The electrical performance of the memories, including read and write characteristics, is described and the long-term stability of the less stable ‘0’ state is studied in different environmental conditions. The memories can find use in low-cost mass printing applications.

Flexible organic tandem solar modules with 6% efficiency: combining roll-to-roll compatible processing with high geometric fill factors

DEFF Research Database (Denmark)

Spyropoulos, G. D.; Kubis, P.; Li, Na

2014-01-01

Organic solar cell technology bears the potential for high photovoltaic performance combined with truly low-cost, high-volume processing. Here we demonstrate organic tandem solar modules on flexible substrates fabricated by fully roll-to-roll compatible processing at temperatures...

Microstructure evolution during spray rolling and heat treatment of 2124 Al

Energy Technology Data Exchange (ETDEWEB)

McHugh, K.M. [Industrial Technology Department, Idaho National Laboratory, Idaho Falls, ID 83415-2050 (United States)], E-mail: kevin.mchugh@inl.gov; Lin, Y.; Zhou, Y.; Johnson, S.B.; Delplanque, J.-P.; Lavernia, E.J. [Department of Chemical Engineering and Materials Science, University of California, Davis, CA 95616 (United States)

2008-03-25

Spray rolling is a strip-casting technology that combines elements of spray forming and twin-roll casting. It consists of atomizing molten metal with a high velocity inert gas, quenching the resultant droplets in flight, and directing the spray between mill rolls. In-flight convection heat transfer from atomized droplets and conduction heat transfer at the rolls rapidly remove the metal's latent heat. Hot deformation of the semi-solid material in the rolls results in fully consolidated, rapidly-solidified strip. Spray rolling operates at a higher solidification rate than conventional twin-roll casting and is able to process a broader range of alloys at high production rates. A laboratory-scale strip caster was constructed and used to evaluate the interplay of processing parameters and strip quality for strips up to 200 mm wide and 1.6-6.4 mm thick. This paper examines microstructure evolution during spray rolling and explores how gas-to-metal mass flow ratio influences the microstructure and mechanical properties of spray-rolled 2124 Al. The influences of solution heat treatment and cold rolling on grain structure and constituent particle spheroidization are also examined.

Small-angle X-ray titration study on the complex formation between 5-S RNA and the L18 protein of the Escherichia coli 50-S ribosome particle

International Nuclear Information System (INIS)

Oesterberg, R.; Garrett, A.

1977-01-01

The 5-S RNA (A) and the L 18 protein (B) from Escherichia coli ribosomes form one single AB complex in the concentration ranges supposed to prevail in vivo; at concentrations of L 18 higher than 40 mM there is some indication for a minor species, most probably an AB 2 species. This is indicated from the X-ray scattering titration data of the 5-S RNA/L 18 system recorded at 21 0 C in ribosomal reconstitution buffer. As a result of the 1 : 1 complex formation, there is a relatively small but defined increase in the radius of gyration from 3.61 to 3.85 nm. This result as well as the experimental scattering curve can be explained by models where it is assumed that the elongated L 18 model is quite far from the electron density centre and where protein L 18 interacts with one or both of the minor arms of the supposed Y-shaped 5-S RNA molecule. (orig.) [de

Grease lubrication in rolling bearings

CERN Document Server

Lugt, Piet M

2012-01-01

The definitive book on the science of grease lubrication for roller and needle bearings in industrial and vehicle engineering. Grease Lubrication in Rolling Bearings provides an overview of the existing knowledge on the various aspects of grease lubrication (including lubrication systems) and the state of the art models that exist today. The book reviews the physical and chemical aspects of grease lubrication, primarily directed towards lubrication of rolling bearings. The first part of the book covers grease composition, properties and rheology, including thermal

Cold rolling precision forming of shaft parts theory and technologies

CERN Document Server

Song, Jianli; Li, Yongtang

2017-01-01

This book presents in detail the theory, processes and equipment involved in cold rolling precision forming technologies, focusing on spline and thread shaft parts. The main topics discussed include the status quo of research on cold rolling precision forming technologies; the design and calculation of process parameters; the numerical simulation of cold rolling forming processes; and the equipment used in cold rolling forming. The mechanism of cold rolling forming is extremely complex, and research on the processes, theory and mechanical analysis of spline cold rolling forming has remained very limited to date. In practice, the forming processes and production methods used are mainly chosen on the basis of individual experience. As such, there is a marked lack of both systematic, theory-based guidelines, and of specialized books covering theoretical analysis, numerical simulation, experiments and equipment used in spline cold rolling forming processes – all key points that are included in this book and ill...

The influence of the roll diameter in flat rolling of of superconducting in situ and ex situ MgB2 tape

DEFF Research Database (Denmark)

Hancock, Michael Halloway; Bay, Niels

2007-01-01

, 150 and 210 mm in each step. The investigation has shown that the in situ powder is more readily compacted than the ex situ powder, with an average increase of relative density after mechanical processing of 37% for in situ powder and 19% for ex situ powder. Statistical analysis showed that the choice......Applying the powder in tube (PIT) method, single-filament MgB2/Fe wire and tape has been manufactured applying both the ex situ and the in situ approach. The influence of the roll diameter in three-step flat rolling on the powder density and critical temperature has been examined using rolls of 70...... roll in the first and second reductions followed by the 150 mm or 210 mm roll in the last reduction was the optimum strategy for both powder types. AC susceptibility testing showed that for the in situ tapes there was no correlation between the powder density and the critical temperature. For ex situ...

MicroRNA-target gene responses to lead-induced stress in cotton (Gossypium hirsutum L.).

Science.gov (United States)

He, Qiuling; Zhu, Shuijin; Zhang, Baohong

2014-09-01

MicroRNAs (miRNAs) play key roles in plant responses to various metal stresses. To investigate the miRNA-mediated plant response to heavy metals, cotton (Gossypium hirsutum L.), the most important fiber crop in the world, was exposed to different concentrations (0, 25, 50, 100, and 200 µM) of lead (Pb) and then the toxicological effects were investigated. The expression patterns of 16 stress-responsive miRNAs and 10 target genes were monitored in cotton leaves and roots by quantitative real-time PCR (qRT-PCR); of these selected genes, several miRNAs and their target genes are involved in root development. The results show a reciprocal regulation of cotton response to lead stress by miRNAs. The characterization of the miRNAs and the associated target genes in response to lead exposure would help in defining the potential roles of miRNAs in plant adaptation to heavy metal stress and further understanding miRNA regulation in response to abiotic stress.

Rolling induced size effects in elastic–viscoplastic sheet metals

DEFF Research Database (Denmark)

Nielsen, Kim Lau

2015-01-01

sheet rolling, where a non-homogeneous material deformation takes place between the rollers. Large strain gradients develop where the rollers first come in contact with the sheet, and a higher order plasticity model is employed to illustrate their influence at small scales. The study reveals...... presented revolves around the rolling induced effect of visco-plasticity (ranging hot and cold rolling) in combination with strain gradient hardening – including both dissipative and energetic contributions. To bring out first order effects on rolling at small scale, the modeling efforts are limited to flat...

Entry Strategies to Indonesian Market Case: MoveaRoll

OpenAIRE

Islam, Zahedul

2013-01-01

MoveRoll Oy, an internationally growing and expanding Finnish company located in Porvoo, has invented a unique technology to produce a paper roll conveyor which the company believes will revolutionise the paper roll handling industry. Moreover, after establishing itself in the Finnish market, the company has started expanding its business to countries like Sweden, USA, and China. Likewise, the process of expanding to the Russian market is currently underway and the company has recently launch...

Multifunctional triblock co-polymer mP3/4HB-b-PEG-b-lPEI for efficient intracellular siRNA delivery and gene silencing.

Science.gov (United States)

Zhou, Li; Chen, Zhifei; Wang, Feifei; Yang, Xiuqun; Zhang, Biliang

2013-04-01

A non-viral siRNA carrier composed of mono-methoxy-poly (3-hydroxybutyrate-co-4-hydroxybutyrate)-block-polyethylene glycol-block-linear polyethyleneimine (mP3/4HB-b-PEG-b-lPEI) was synthesized using 1800 Da linear polyethyleneimine and evaluated for siRNA delivery. Our study demonstrated that siRNA could be efficiently combined with mP3/4HB-b-PEG-b-lPEI (mAG) co-polymer and was protected from nuclease degradation. The combined siRNA were released from the complexes easily under heparin competition. The particle size of the mAG/siRNA complexes was 158 nm, with a ζ-potential of around 28 mV. Atomic force microscopy images displayed spherical and homogeneously distributed complexes. The mAG block co-polymer displayed low cytotoxicity and efficient cellular uptake of Cy3-siRNA in A549 cells by flow cytometry and confocal microscopy. In vitro transfection efficiency of the block co-polymer was assessed using siRNA against luciferase in cultured A549-Luc, HeLa-Luc, HLF-Luc, A375-Luc and MCF-7-Luc cells. A higher transfection efficiency and lower cytotoxicity was obtained by mAG block co-polymer in five cell lines. Furthermore, a remarkable improvement in luciferase gene silencing efficiency of the mAG complex (up to 90-95%) over that of Lipofectamine™ 2000 (70-82%) was observed in HLF-Luc and A375-Luc cells. Additionally, a mAG/p65-siRNA complex also showed a better capability than Lipofectamine™ 2000/p65-siRNA complex to drastically reduce the p65 mRNA level down to 10-16% in HeLa, U251 and HUVEC cells at an N/P ratio of 70. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

14 CFR 25.493 - Braked roll conditions.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Braked roll conditions. 25.493 Section 25.493 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Structure Ground Loads § 25.493 Braked roll conditions. (a...

Evolution of fuel plate parameters during deformation in rolling

Energy Technology Data Exchange (ETDEWEB)

Durazzo, M., E-mail: mdurazzo@ipen.br [Nuclear and Energy Research Institute – IPEN/CNEN-SP, São Paulo (Brazil); Vieira, E.; Urano de Carvalho, E.F. [Nuclear and Energy Research Institute – IPEN/CNEN-SP, São Paulo (Brazil); Riella, H.G. [Nuclear and Energy Research Institute – IPEN/CNEN-SP, São Paulo (Brazil); Chemical Engineering Department, Santa Catarina Federal University, Florianópolis (Brazil)

2017-07-15

The Nuclear and Energy Research Institute – IPEN/CNEN-SP routinely produces the nuclear fuel necessary for operating its research reactor, IEA-R1. This fuel consists of fuel plates containing U{sub 3}Si{sub 2}-Al composites as the meat, which are fabricated by rolling. The rolling process currently deployed was developed based on information obtained from literature, which was used as a premise for defining the current manufacturing procedures, according to a methodology with an essentially empirical character. Despite the current rolling process being perfectly stable and highly reproducible, it is not well characterized and is therefore not fully known. The objective of this work is to characterize the rolling process for producing dispersion fuel plates. Results regarding the evolution of the main parameters of technological interest, after each rolling pass, are presented. Some defects that originated along the fuel plate deformation during the rolling process were characterized and discussed. The fabrication procedures for manufacturing the fuel plates are also presented. - Highlights: •Evolution of defects when manufacturing dispersion fuel plates. •Aspects of dispersion fuel plates fabrication. •What happen during the manufacturing of dispersion fuel plates? •Clarifying the deformation of fuel plates by rolling.

Highly flexible indium zinc oxide electrode grown on PET substrate by cost efficient roll-to-roll sputtering process

International Nuclear Information System (INIS)

Park, Yong-Seok; Kim, Han-Ki; Jeong, Soon-Wook; Cho, Woon-Jo

2010-01-01

We have investigated the characteristics of flexible indium zinc oxide (IZO) electrode grown on polyethylene terephthalate (PET) substrates using a specially designed roll-to-roll (RTR) sputtering system for use in flexible optoelectronics. It was found that both electrical and optical properties of the flexible IZO electrode were critically dependent on the DC power and Ar/O 2 flow ratio during the roll-to-roll sputtering process. At optimized conditions (constant working pressure of 3 mTorr, Ar/O 2 flow ratio of Ar at only 30 sccm, DC power 800 W and rolling speed at 0.1 cm/s) the flexible IZO electrode exhibits a sheet resistance of 17.25 Ω/sq and an optical transmittance of 89.45% at 550 nm wavelength. Due to the low PET substrate temperature, which is effectively maintained by cooling drum system, all IZO electrodes showed an amorphous structure regardless of the DC power and Ar/O 2 flow ratio. Furthermore, the IZO electrodes grown at optimized condition exhibited superior flexibility than the conventional amorphous ITO electrodes due to its stable amorphous structure. This indicates that the RTR sputter grown IZO electrode is a promising flexible electrode that can substitute for the conventional ITO electrode, due to its low resistance, high transparency, superior flexibility and fast preparation by the RTR process.

Influence exerted by the shape of the surfaces of working roll barrels upon the course of the MEFASS (Metal Forming Aided by Shear Stresses rolling process

Directory of Open Access Journals (Sweden)

Świątoniowski A.

2017-03-01

Full Text Available The essential aspect of the MEFASS rolling process is introducing the cyclic axial counter movement of the rolls transverse to the direction of rolling in the course of a band pass through a rolling gap. The effect of a change in the way of deformation obtained in this manner makes it possible to set in one roll pass a deformation several times larger than it is possible in a conventional process. In this paper, upon the basis of the computer model of the MES process, supported by experimental research, the analysis of the influence exerted by the shape of the surface of roll barrels upon the distribution of the intensity of stresses σi and deformations εi in the section of the band being rolled, and also upon the kinematic and force parameters of the process.

Lubrication in cold rolling : Numerical simulation using multigrid techniques

NARCIS (Netherlands)

Lugt, Pieter Martin

1992-01-01

In the cold rolling process a lubricant is applied on the rolls and/or the strip mate­rial. Due to the velocities of the rolls and the strip, part of the lubricant is sheared into the contact causing, amongst others, a reduction of the friction. In this thesis a physical-mathematical model is

21 CFR 136.130 - Milk bread, rolls, and buns.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Milk bread, rolls, and buns. 136.130 Section 136.130 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED....130 Milk bread, rolls, and buns. (a) Each of the foods milk bread, milk rolls, and milk buns conforms...

3-dimensional numerical modelling of rolling of superconducting Ag/BSCCO tape

DEFF Research Database (Denmark)

Eriksen, Morten; Bech, Jakob Ilsted; Seifi, Behrouz

2000-01-01

and Ø126 mm) have been investigated. It is found that it is possible to perform numerical simulation with 3D models of flat rolling of multifilament wire. Two 3D models have been used; 3D pressing with rolls and 3D rolling. 3D pressing with rolls have the advance that the simulation time is lower than...... in the 3D rolling. The 3D models have the advantage compared to 2D pressing that they can predict the 3 dimensional flow in the flat rolling, which has been showed to be very imported for the super conduction properties......Numerical simulation of the deformation process during flat rolling of multifilament HTS tapes has been investigated using a commercial FEM program, ELFEN. The numerical models were built up in 2D and 3D using a Drucker-Prager/Cap model for the powder. Three different roll diameters (Ø24 mm, Ø85 mm...

Roll-to-roll printed resistive WORM memory on a flexible substrate

International Nuclear Information System (INIS)

Leppäniemi, Jaakko; Mattila, Tomi; Suhonen, Mika; Alastalo, Ari; Kololuoma, Terho

2012-01-01

The fabrication process and the operation characteristics of a fully roll-to-roll printed resistive write-once-read-many memory on a flexible substrate are presented. The low-voltage (<10 V) write operation of the memories from a high resistivity ‘0’ state to a low resistivity ‘1’ state is based on the rapid electrical sintering of bits containing silver nanoparticles. The bit ink is formulated by mixing two commercially available silver nanoparticle inks in order to tune the initial square resistance of the bits and to create a self-organized network of percolating paths. The electrical performance of the memories, including read and write characteristics, is described and the long-term stability of the less stable ‘0’ state is studied in different environmental conditions. The memories can find use in low-cost mass printing applications. (paper)

Study Friction Distribution during the Cold Rolling of Material by Matroll Software

International Nuclear Information System (INIS)

Abdollahi, H.; Dehghani, K.

2007-01-01

Rolling process is one of the most important ways of metal forming. Since the results of this process are almost finished product, therefore controlling the parameters affecting this process is very important in order to have cold rolling products with high quality. Among the parameters knowing the coefficient of friction within the roll gap is known as the most significant one. That is because other rolling parameters such as rolling force, pressure in the roll gap, forward slip, surface quality of sheet, and the life of work rolls are directly influenced by friction. On the other hand, in rolling calculation due to lake of a true amount for coefficient of friction a supposed value is considered for it. In this study, a new software (Matroll), is introduced which can determine the coefficient of friction (COF) and plot the friction hills for an industrial mill. Besides, based on rolling equations, it offers about 30 rolling parameters as outputs. Having the rolling characteristics as inputs, the software is able to calculate the coefficient of friction. Many rolling passes were performed on real industrial aluminum mill. The coefficient of friction was obtained for all passes. The results are in good agreement with the findings of the other researchers

A Scalable Route to Nanoporous Large-Area Atomically Thin Graphene Membranes by Roll-to-Roll Chemical Vapor Deposition and Polymer Support Casting.

Science.gov (United States)

Kidambi, Piran R; Mariappan, Dhanushkodi D; Dee, Nicholas T; Vyatskikh, Andrey; Zhang, Sui; Karnik, Rohit; Hart, A John

2018-03-28

Scalable, cost-effective synthesis and integration of graphene is imperative to realize large-area applications such as nanoporous atomically thin membranes (NATMs). Here, we report a scalable route to the production of NATMs via high-speed, continuous synthesis of large-area graphene by roll-to-roll chemical vapor deposition (CVD), combined with casting of a hierarchically porous polymer support. To begin, we designed and built a two zone roll-to-roll graphene CVD reactor, which sequentially exposes the moving foil substrate to annealing and growth atmospheres, with a sharp, isothermal transition between the zones. The configurational flexibility of the reactor design allows for a detailed evaluation of key parameters affecting graphene quality and trade-offs to be considered for high-rate roll-to-roll graphene manufacturing. With this system, we achieve synthesis of uniform high-quality monolayer graphene ( I D / I G casting and postprocessing, show size-selective molecular transport with performance comparable to that of membranes made from conventionally synthesized graphene. Therefore, this work establishes the feasibility of a scalable manufacturing process of NATMs, for applications including protein desalting and small-molecule separations.

New progress of FEM simulation and AI application in rolling at RAL

International Nuclear Information System (INIS)

Liu Xianghua; Wang Guodong; Zhao Kun

2000-01-01

New progresses on FEM simulation and AI application in rolling have been achieved at RAL recently. The existence and uniqueness of the extreme point of total functional for rolling problem has been proved. Different rolling processes, such as H-beam rolling, ribbing strip rolling, slab sizing, have been solved by our in-house FEM software package. The simulation results have been put into production use to improve the precision of math models. The Artificial Neural Network has been used to predict rolling force, coiling temperature, microstructure and properties of the rolled products. An expert system for deviation diagnoses of strip thickness has been developed for industry use. Synergetic Artificial Intelligence has also been applied to rolling scheduling. We are making continuous efforts to develop AI applications for rolling line co-operating in China steel industry. (author)

Kangur Ivan ajab triipu, naised lähevad arust ära / Rein Sikk

Index Scriptorium Estoniae

Sikk, Rein, 1961-

2011-01-01

Ivan Uvarovist, kes koob oma pisikeses töökojas Rakveres rahvarõivamustritega puuvillast triibukangast, mille järele on suur nõudmine. Ivan Uvarov on töötanud 30 aastat kangastelgede mehaanikuna

Multilayer Steel Materials Deformation Resistance and Roll Force Measurement

Directory of Open Access Journals (Sweden)

A. G. Kolesnikov

2014-01-01

Full Text Available To create new types of cars, raise their reliability, gain operational life, and decrease in metal consumption of products it is necessary to improve mechanical, physical, and also special properties of the constructional materials applied in mechanical engineering. Presently, there are intensive researches and developments under way to create materials with ultrafine-grained structure (the sizes of grains in their crystal lattice make less than 1 micron in one of the measurements.BMSTU developed a manufacturing technology of multilayer steel sheets with steady ultrafine-grained structure based on the multiple hot rolling of billet as a composition consisting of the alternating metal sheets. A principled condition for implementation of such technology is existence of different crystallographic modifications in the adjoining sheets of the composition at specified temperature of rolling.Power parameters of rolling are important technical characteristics of the process. Usually, to determine a deformation resistance value when rolling the diverse multilayer materials, is used the actual resistance value averaging in relation to the components of the composition. The aim of this work is a comparative analysis of known calculated dependences with experimental data when rolling the 100-layer samples. Objects of research were the 100-layer compositions based on the alternating layers of steel 08H18N10 and U8.Experimental samples represented the vacuumized capsules with height, width, and length of 53 mm x 53 mm x 200 mm, respectively, in which there were the 100-layer packs from sheets, each of 0.5 mm, based on the composition of steels (U8+08H18N10. Rolling was made on the double-high mill with rolls of 160 mm in diameter during 19 passes to the thickness of 7 mm with the speed of 0,1 m/s. Relative sinking in each pass was accepted to be equal 10±2,5%. Rolling forces were measured by the strain-gauging method using the measuring cells, located under

Continuous roll-to-roll a-Si photovoltaic manufacturing technology. Final subcontract report, 1 April 1992--30 September 1995

Energy Technology Data Exchange (ETDEWEB)

Izu, M. [Energy Conversion Devices, Inc., Troy, MI (US)

1996-02-01

ECD has made important progress in the development of materials, device designs, and manufacturing processes required for the continued advancement of practical photovoltaic technology{sub 1-23}. ECD has pioneered and continues further development of two key proprietary technologies, with significant potential for achieving the cost goals necessary for widespread growth of the photovoltaic market: (1) a low cost, roll-to- roll continuous substrate thin-film solar cell manufacturing process; (2) a high efficiency, monolithic, multiple-junction, spectrum- splitting thin-film amorphous silicon alloy device structure. Commercial production of multiple-junction a-Si alloy modules has been underway at ECD and its joint venture company for a number of years using ECD's proprietary roll-to-roll process and numerous advantages of this technology have been demonstrated. These include relatively low semiconductor material cost, relatively low process cost, a light-weight, rugged and flexible substrate that results in lowered installed costs of PV systems, and environmentally safe materials. Nevertheless, the manufacturing cost per watt of PV modules from our current plant remains high. In order to achieve high stable efficiency and low manufacturing cost, ECD has, at ECD's expense, engineered and constructed a 2 MW production line and a 200 kW pilot line, incorporating earlier ECD research advances in device efficiency through the use of multi-junction spectrum-splitting and high performance back-reflector cell design. Under this subcontract six tasks were directed towards achieving this goal. They are: Task I: Optimization of back-reflector system; Task II: Optimization of the Si-Ge narrow bandgap solar cells; Task III: Optimization of the stable efficiency of photovoltaic modules; Task IV: Demonstration of serpentine web continuous roll-to-roll deposition technology; Task V: Material cost reductions; and Task VI: Improving the module assembly process.

Effects of F virus in Bombyx mori L., 1758 (Lep., bombycidae) and site determination of the viral RNA synthesis by auto-radiography

International Nuclear Information System (INIS)

Almeida, I.M.G. de.

1980-01-01

Some aspects of the pathological process of the infectious flacherie virus in the midgut epithelial cells of the silkworm (Bombyx mori L., 1758 (Lep., Bombycidae)) as well as the site of the viral RNA synthesis were investigated. Electron microscope observation of these inclusion bodies showed that they were surrounded by a single membrane and containing many vesicles and virus particles. The nucleus of the cells infected with the virus exhibited higher electron dense masses than the healthy ones. The site of the viral RNA synthesis. Actinomycin D, at the used concentration, selectively blocked the cell RNA synthesis without affecting the virus RNA synthesis. The inhibition found was about 60%. The data obtained indicated that the viral RNA synthesis occurs in the nucleus of the midgut epithelial cells of the silkworm larvae. (author)

A life cycle analysis of polymer solar cell modules prepared using roll-to-roll methods under ambient conditions

DEFF Research Database (Denmark)

Espinosa Martinez, Nieves; García-Valverde, Rafael; Urbina, Antonio

2011-01-01

A life cycle analysis was performed on a full roll-to-roll coating procedure used for the manufacture of flexible polymer solar cell modules. The process known as ProcessOne employs a polyester substrate with a sputtered layer of the transparent conductor indium-tin-oxide (ITO). The ITO film was ...... photovoltaic technologies. The results showed that an Energy Pay-Back Time (EPBT) of 2.02 years can be achieved for an organic solar module of 2% efficiency, which could be reduced to 1.35 years, if the efficiency was 3%.......A life cycle analysis was performed on a full roll-to-roll coating procedure used for the manufacture of flexible polymer solar cell modules. The process known as ProcessOne employs a polyester substrate with a sputtered layer of the transparent conductor indium-tin-oxide (ITO). The ITO film...... printed. Finally the polymer solar modules were encapsulated, using a polyester barrier material. All operations except the application of ITO were carried out under ambient conditions. The life cycle analysis delivered a material inventory of the full process for a module production...

Rolled-up inductor structure for a radiofrequency integrated circuit (RFIC)

Science.gov (United States)

Li, Xiuling; Huang, Wen; Ferreira, Placid M.; Yu, Xin

2015-12-29

A rolled-up inductor structure for a radiofrequency integrated circuit (RFIC) comprises a multilayer sheet in a rolled configuration comprising multiple turns about a longitudinal axis. The multilayer sheet comprises a conductive pattern layer on a strain-relieved layer, and the conductive pattern layer comprises at least one conductive strip having a length extending in a rolling direction. The at least one conductive strip thereby wraps around the longitudinal axis in the rolled configuration. The conductive pattern layer may also comprise two conductive feed lines connected to the conductive strip for passage of electrical current therethrough. The conductive strip serves as an inductor cell of the rolled-up inductor structure.

Rolled-up nanotechnology: 3D photonic materials by design

International Nuclear Information System (INIS)

Böttner, Stefan; Jorgensen, Matthew R.; Schmidt, Oliver G.

2016-01-01

Rolled-up nanotechnology involves the deposition of strained material layers for subsequent release and relaxation into functional structures with applications spanning several disciplines. Originally developed for use with semiconductor materials, over the last decade the processes involved in rolled-up nanotechnology have been applied across a wide palette of materials resulting in applications (among others) in micro robotics, energy storage, electronics, and photonics. Here we highlight the key advancements and future directions in rolled-up photonics, focusing on the diverse demonstrations of rolled-up three-dimensional microresonators which enable integrated sensing, micro-lasing, and out-of-plane routing of light.

Mathematical modeling of a process the rolling delivery

Science.gov (United States)

Stepanov, Mikhail A.; Korolev, Andrey A.

2018-03-01

An adduced analysis of the scientific researches in a domain of the rolling equipments, also research of properties the working material. A one of perspective direction of scientific research this is mathematical modeling. That is broadly used in many scientific disciplines and especially at the technical, applied sciences. With the aid of mathematical modeling it can be study of physical properties of the researching objects and systems. A research of the rolling delivery and transporting devices realized with the aid of a construction of mathematical model of appropriate process. To be described the basic principles and conditions of a construction of mathematical models of the real objects. For example to be consider a construction of mathematical model the rolling delivery device. For a construction that is model used system of the equations, which consist of: Lagrange’s equation of a motion, describing of the law conservation of energy of a mechanical system, and the Navier - Stokes equations, which characterize of the flow of a continuous non-compressed fluid. A construction of mathematical model the rolling deliver to let determined of a total energy of device, and therefore to got the dependence upon the power of drive to a gap between of rolls. A corroborate the hypothesis about laminar the flow of a material into the rolling gap of deliver.

Effect of Rolling Resistance in Dem Models With Spherical Bodies

Directory of Open Access Journals (Sweden)

Dubina Radek

2016-12-01

Full Text Available The rolling resistance is an artificial moment arising on the contact of two discrete elements which mimics resistance of two grains of complex shape in contact rolling relatively to each other. The paper investigates the influence of rolling resistance on behaviour of an assembly of spherical discrete elements. Besides the resistance to rolling, the contacts between spherical particles obey the Hertzian law in normal straining and Coulomb model of friction in shear.

Diagnostic capability of 100-mm roll film digitizer system

International Nuclear Information System (INIS)

Kono, M.; Yamasaki, K.; Ikeda, M.

1988-01-01

The authors recently developed a roll film digitizer system. The sampling pitch is 100 μm. a scientific image study (Barger phantom, Hawlet chart, and modulation transfer function), receiver operating characteristic (ROC) study, and clinical evaluation were performed. For the ROC study, a chest phantom with simulated nodule and hairline was used. Differences in accuracy between readings of cathode ray tube (CRT) images and roll film images were not statistically significant for hairline and coin lesions, P<.05). As a clinical evaluation, eight radiologists evaluated predetermined radiologic findings on a five-point rating scale compared with original roll film images. Scores for CRT images were equal to those for the original roll film images. In conclusion, CRT images (1,000 x 1,000) were found to be sufficient for diagnostic accuracy of chest images compared with roll film images (100 x 100 mm)

Alteration of RNA splicing by small molecule inhibitors of the interaction between NHP2L1 and U4

Science.gov (United States)

Diouf, Barthelemy; Lin, Wenwei; Goktug, Asli; Grace, Christy R. R.; Waddell, Michael Brett; Bao, Ju; Shao, Youming; Heath, Richard J.; Zheng, Jie J.; Shelat, Anang A.; Relling, Mary V.; Chen, Taosheng; Evans, William E.

2018-01-01

Splicing is an important eukaryotic mechanism for expanding the transcriptome and proteome, influencing a number of biological processes. Understanding its regulation and identifying small molecules that modulate this process remains a challenge. We developed an assay based on time-resolved FRET (TR-FRET) to detect the interaction between the protein NHP2L1 and U4 RNA, which are two key components of the spliceosome. We used this assay to identify small molecules that interfere with this interaction in a high-throughput screening (HTS) campaign. Topotecan and other camptothecin derivatives were among the top hits. We confirmed that topotecan disrupts the interaction between NHP2L1 and U4 by binding to U4 and inhibits RNA splicing. Our data reveal new functions of known drugs which could facilitate the development of therapeutic strategies to modify splicing and alter gene function. PMID:28985478

Hot-rolling metals in vacuum. Information circular

International Nuclear Information System (INIS)

Beall, R.A.; Worthington, R.B.; Blickensderfer, R.

1979-01-01

The process of hot-rolling metals, alloys, and composites in vacuum is studied. First, a comprehensive review of the literature is presented, including the advantages and disadvantages of using vacuum. Next, details of hot-rolling titanium, chromium, and molybdenum-iron bimetal are given. Finally, the design of new equipment is described

Roll compaction and granulation system for nuclear fuel material

International Nuclear Information System (INIS)

Goldmann, L.H. Jr.; Holley, C.C.

1981-01-01

A roll compaction and roll granulation system has been designed and fabricated to replace conventional preslugging and crushing operations typically used in the fabrication of mixed oxide nuclear fuel pellets. This equipment will be of maintenance advantage with only the compaction and granulation rolls inside containment. The prototype is being tested and the results will be reported within a year

Circular RNA (circRNA) was an important bridge in the switch from the RNA world to the DNA world.

Science.gov (United States)

Soslau, Gerald

2018-06-14

osmolarity. Circular RNA, circRNA, is proposed as a critical stable RNA molecule that served as the genetic precursor for the switch to DNA and the replication of circRNA by a rolling circle mechanism gave rise to the RNA complexity required for the genetic functions of the cell. The replicating ribocyte would have required protein synthesis as well as RNA replication and a model for non-coded and primordial coded protein synthesis is proposed. Finally, the switch from the RNA to the DNA world would have involved the synthesis of an RNA:DNA hybrid prior to the formation of dsDNA. If the hybrid was a circular molecule that ultimately yielded a circular dsDNA molecule, it could predict that the primordial DNA cell would evolve into a bacterial cell with a single circular chromosome. One would hope that continued speculation of the origin of life will spur new directions of research that may never fully answer the questions of the past but add to our ability to regulate potentially harmful biological events in the present and in the future. Copyright © 2018 Elsevier Ltd. All rights reserved.

Magnon inflation: slow roll with steep potentials

Energy Technology Data Exchange (ETDEWEB)

Adshead, Peter [Department of Physics, University of Illinois at Urbana-Champaign,Urbana, IL 61801 (United States); Blas, Diego [Theoretical Physics Department, CERN,CH-1211 Geneva 23 (Switzerland); Burgess, C.P.; Hayman, Peter [Physics & Astronomy, McMaster University,Hamilton, ON, L8S 4M1 (Canada); Perimeter Institute for Theoretical Physics, Waterloo, Ontario N2L 2Y5 (Canada); Patil, Subodh P. [Department of Theoretical Physics, University of Geneva,24 Quai Ansermet, Geneva, CH-1211 (Switzerland)

2016-11-04

We find multi-scalar effective field theories (EFTs) that can achieve a slow inflationary roll despite having a scalar potential that does not satisfy G{sup ab}∂{sub a}V∂{sub b}V≪V{sup 2}/M{sub p}{sup 2} (where G{sub ab} is the target-space metric). They evade the usual slow-roll conditions on V because their kinetic energies are dominated by single-derivative terms rather than the usual two-derivative terms. Single derivatives dominate during slow roll and so do not require a breakdown of the usual derivative expansion that underpins calculational control in much of cosmology. The presence of such terms requires some sort of UV Lorentz-symmetry breaking during inflation (besides the usual cosmological breaking). Chromo-natural inflation provides one particular example of a UV theory that can generate the multi-field single-derivative terms we consider, and we argue that the EFT we find indeed captures the slow-roll conditions for its background evolution. We also show that our EFT can be understood as a multi-field generalization of the single-field Cuscuton models. The multi-field case introduces a new feature, however: the scalar kinetic terms define a target-space 2-form, F{sub ab}, whose antisymmetry gives new ways for slow roll to be achieved.

Prediction of Parametric Roll Resonance by Multilayer Perceptron Neural Network

DEFF Research Database (Denmark)

Míguez González, M; López Peña, F.; Díaz Casás, V.

2011-01-01

Parametric roll resonance is a ship stability related phenomenon that generates sudden large amplitude oscillations up to 30-40 degrees of roll. This can cause severe damage, and it can put the crew in serious danger. The need for a parametric rolling real time prediction system has been acknowle......Parametric roll resonance is a ship stability related phenomenon that generates sudden large amplitude oscillations up to 30-40 degrees of roll. This can cause severe damage, and it can put the crew in serious danger. The need for a parametric rolling real time prediction system has been...... acknowledged in the last few years. This work proposes a prediction system based on a multilayer perceptron (MP) neural network. The training and testing of the MP network is accomplished by feeding it with simulated data of a three degrees-of-freedom nonlinear model of a fishing vessel. The neural network...

Nõukogude kunst ja lääne vaatepunktid / Heie Treier

Index Scriptorium Estoniae

Treier, Heie, 1963-

2006-01-01

Muljeid Kaunases Vytautas Suure ülikoolis 26.-27. oktoobril 2006 a. toimunud rahvusvaheliselt konverentsilt "Kunst ja poliitika : Ida-Euroopa üksikjuhtumid". Eestist esinesid ettekannetega Heie Treier ja Andres Kurg. Lähemalt inglanna Deborah Lewer'i ettekandest saksa maalikunstniku Willi Sitte kohta. Leedu punamonumentide pargist Grutas Park

Fluid management in roll-to-roll nanoimprint lithography

Science.gov (United States)

Jain, A.; Bonnecaze, R. T.

2013-06-01

The key process parameters of UV roll-to-roll nanoimprint lithography are identified from an analysis of the fluid, curing, and peeling dynamics. The process includes merging of droplets of imprint material, curing of the imprint material from a viscous liquid to elastic solid resist, and pattern replication and detachment of the resist from template. The time and distances on the web or rigid substrate over which these processes occur are determined as function of the physical properties of the uncured liquid, the cured solid, and the roller configuration. The upper convected Maxwell equation is used to model the viscoelastic liquid and to calculate the force on the substrate and the torque on the roller. The available exposure time is found to be the rate limiting parameter and it is O(√Rho /uo), where R is the radius of the roller, ho is minimum gap between the roller and web, and uo is the velocity of the web. The residual layer thickness of the resist should be larger than the gap between the roller and the substrate to ensure complete feature filling and optimal pattern replication. For lower residual layer thickness, the droplets may not merge to form a continuous film for pattern transfer.

Biology of Byctiscus populi (L. (Coleoptera, Attelabidae. Part I. Last year’s imagoes

Directory of Open Access Journals (Sweden)

Jaroslav Urban

2012-01-01

Full Text Available The biology of Byctiscus populi (L. was studied on Populus tremula L. in Forest District Bílovice nad Svitavou (former district of Brno-venkov in the period 2007 to 2009. Last year’s imagoes occurred there from the third decade of April until the end of July. Females lived in the laboratory on average 1.5 months and damaged 7.2 to 19.2 cm2 leaves. In nature, females live on average two months. They produce 20 to 30 leafrolls and lay 30 to 41 eggs. Every day, they roll up 0 to 4 (on average 0.4 leafrolls and lay 0 to 6 (on average 0.8 eggs. They lay 0 to 4 (on average 1.3 eggs into one leafroll, in the laboratory 0 to 8 (on average 2.2 eggs. In 67% leafrolls, leaves are rolled up by their adaxial face outwards, in 32% inward and in 1% towards both faces. The production of rolls (from the beginning of biting out holes into petioles to leafroll sticking takes on average two hours. Two thirds of rolls persist on shoots for the period of 1 to 4 weeks. At one third of rolls, females bite out the petioles immediately after rolling up the leaf blades and rolls fall to the soil surface.1

Reduction in deformed wing virus infection in larval and adult honey bees (Apis mellifera L.) by double-stranded RNA ingestion.

Science.gov (United States)

Desai, S D; Eu, Y-J; Whyard, S; Currie, R W

2012-08-01

Deformed wing virus (DWV) is a serious pathogen of the honey bee, Apis mellifera L., vectored by the parasitic mite Varroa destructor. The virus is associated with wing deformity in symptomatic bees, and premature death and reduced colony performance in asymptomatic bees. In the present study we reduced DWV infection by feeding both first instar larvae and adult A. mellifera with a double-stranded (ds) RNA construct, DWV-dsRNA, which is specific to DWV in DWV-inoculated bees, by mixing it with their food. We showed that feeding DWV to larvae causes wing deformity in adult bees in the absence of varroa mites and decreases survival rates of adult bees relative to bees not fed DWV. Feeding larvae with DWV-dsRNA in advance of inoculation with virus reduced the DWV viral level and reduced wing deformity relative to larvae fed DWV or DWV with green fluorescent protein-dsRNA (probably a result of RNA silencing), but did not affect survival to the adult stage. Feeding DWV-dsRNA did not affect larval survival rates, which suggests that dsRNA is non-toxic to larvae. Feeding adult workers with DWV-dsRNA in advance of inoculation with virus increased their longevity and reduced DWV concentration relative to controls. © 2012 The Authors. Insect Molecular Biology © 2012 The Royal Entomological Society.

Effects of microalloying on hot-rolled and cold-rolled Q&P steels

Science.gov (United States)

Azevedo de Araujo, Ana Luiza

Third generation advanced high strength steels (AHSS) have been a major focus in steel development over the last decade. The premise of these types of steel is based on the potential to obtain excellent combinations of strength and ductility with low-alloy compositions by forming mixed microstructures containing retained austenite (RA). The development of heat treatments able to achieve the desired structures and properties, such as quenching and partitioning (Q&P) steels, is driven by new requirements to increase vehicle fuel economy by reducing overall weight while maintaining safety and crashworthiness. Microalloying additions of niobium (Nb) and vanadium (V) in sheet products are known to provide strengthening via grain refinement and precipitation hardening and may influence RA volume fraction and transformation behavior. Additions of microalloying elements in Q&P steels have not been extensively studied to date, however. The objective of the present study was to begin to understand the potential roles of Nb and V in hot-rolled and cold-rolled Q&P steel. For that, a common Q&P steel composition was selected as a Base alloy with 0.2C-1.5Si-2.0Mn (wt. %). Two alloys with an addition of Nb (0.02 and 0.04 wt. %) and one with an addition of V (0.06 wt. %) to the Base alloy were investigated. Both hot-rolled and cold-rolled/annealed Q&P simulations were conducted. In the hot-rolled Q&P study, thermomechanical processing was simulated via hot torsion testing in a GleebleRTM 3500, and four coiling temperatures (CT) were chosen. Microstructural evaluation (including RA measurements via electron backscattered diffraction - EBSD) and hardness measurements were performed for all alloys and coiling conditions. The analysis showed that Nb additions led to overall refinement of the prior microstructure. Maximum RA fractions were measured at the 375 °C CT, and microalloying was associated with increased RA in this condition when compared to the Base alloy. A change in

Roll seat belt induced injury of the duodenum.

Science.gov (United States)

Bergqvist, D; Hedelin, H

1976-05-01

A case of duodenal rupture with a roll three-point seatbelt is described. It is apparently the seventh reported case of duodenal rupture in safety belt users. A female driver fell asleep, and her car went off the road, rolling forward in a ditch, slowing slightly, and then came to a sudden stop. The rupture was unusual: on the first part of duodenum, intraperitoneal, and longitudinal. The rupture mechanism is discussed, and the deficiencies of the roll seatbelt pointed out in accidents like the one described.

Early Detection of Parametric Roll Resonance on Container Ships

DEFF Research Database (Denmark)

Galeazzi, Roberto; Blanke, Mogens; Poulsen, Niels Kjølstad

2013-01-01

Parametric roll resonance on ships is a nonlinear phenomenon where waves encountered at twice the natural roll frequency can bring the vessel dynamics into a bifurcation mode and lead to extreme values of roll. Recent years have seen several incidents with dramatic damage to container vessels...... the ship's speed and course, to escape from the bifurcation condition. This paper proposes nonparametric methods to detect the onset of roll resonance and demonstrates their performance. Theoretical conditions for parametric resonance are revisited and are used to develop efficient methods to detect its...... on experimental data from model tests and on data from a container ship crossing the Atlantic during a storm....

Transmission Electron Microscopy of Amorphous Tandem Thin-Film Silicon Modules Produced by A Roll-to-Roll Process on Plastic Foil

DEFF Research Database (Denmark)

Couty, P.; Duchamp, Martial; Söderström, K.

2011-01-01

An improvement of the photo-current is expected when amorphous silicon solar cells are grown on a ZnO texture. A full understanding of the relationship between cell structure and electrical performance is essential for the rapid development of high efficiency VHF-tandem cells on textured substrates...... a control-lost of shape fidelity is used to smooth the texture and make it compatible with subsequent layer growth. Then, we present the electrical performances of the most promising reference solar cell single junction which was obtained on a roll-to-roll foil. Finally, a tandem amorphous/amorphous Si....... At first, we present the systematic study where amorphous cells are grown on ZnO based textures. For varying the texture, the same original master LPCVD ZnO was successively transferred to nickel molds and finally transferred to the plastic foil by roll-to-roll process. From TEM images, we show how...

Hot forging of roll-cast high aluminum content magnesium alloys

Science.gov (United States)

Kishi, Tomohiro; Watari, Hisaki; Suzuki, Mayumi; Haga, Toshio

2017-10-01

This paper reports on hot forging of high aluminum content magnesium alloy sheets manufactured using horizontal twin-roll casting. AZ111 and AZ131 were applied for twin-roll casting, and a hot-forging test was performed to manufacture high-strength magnesium alloy components economically. For twin-roll casting, the casting conditions of a thick sheet for hot forging were investigated. It was found that twin-roll casting of a 10mm-thick magnesium alloy sheet was possible at a roll speed of 2.5m/min. The grain size of the cast strip was 50 to 70µm. In the hot-forging test, blank material was obtained from as-cast strip. A servo press machine with a servo die cushion was used to investigate appropriate forging conditions (e.g., temperature, forging load, and back pressure) for twin-roll casts (TRCs) AZ111 and AZ131. It was determined that high aluminum content magnesium alloy sheets manufactured using twin-roll casting could be forged with a forging load of 150t and a back pressure of 3t at 420 to 430°C. Applying back pressure during hot forging effectively forged a pin-shaped product.

Predicting the wheel rolling resistance regarding important motion parameters using the artificial neural network

Directory of Open Access Journals (Sweden)

F Gheshlaghi

2016-04-01

the analytical and statistical methods. It is expected that the neural network can more accurately predict the rolling resistance. In this study, the neural network for experimental data was trained and the relationship among some parameters of velocity, dynamic load and tire pressure and rolling resistance were evaluated. Materials and Methods: The soil bin and single wheel tester of Biosystem Engineering Mechanics Department of Urmia University was used in this study. This soil bin has 24 m length, 2 m width and 1 m depth including a single-wheel tester and the carrier. Tester consists of four horizontal arms and a vertical arm to vertical load. The S-shaped load cells were employed in horizontal arms with a load capacity of 200 kg and another 500 kg in the vertical arm was embedded. The tire used in this study was a general pneumatic tire (Good year 9.5L-14, 6 ply In this study, artificial neural networks were used for optimizing the rolling resistance by 35 neurons, 6 inputs and 1 output choices. Comparison of neural network models according to the mean square error and correlation coefficient was used. In addition, 60% of the data on training, 20% on test and finally 20% of the credits was allocated to the validation and Output parameter of the neural network model has determined the tire rolling resistance. Finally, this study predicts the effects of changing parameters of tire pressure, vertical load and velocity on rolling resistance using a trained neural network. Results and Discussion: Based on obtained error of Levenberg- Marquardt algorithm, neural network with 35 neurons in the hidden layer with sigmoid tangent function and one neuron in the output layer with linear actuator function were selected. The regression coefficient of tested network is 0.92 which seems acceptable, considering the complexity of the studied process. Some of the input parameters to the network are speed, pressure and vertical load which their relationship with the rolling

Ring rolling process simulation for geometry optimization

Science.gov (United States)

Franchi, Rodolfo; Del Prete, Antonio; Donatiello, Iolanda; Calabrese, Maurizio

2017-10-01

Ring Rolling is a complex hot forming process where different rolls are involved in the production of seamless rings. Since each roll must be independently controlled, different speed laws must be set; usually, in the industrial environment, a milling curve is introduced to monitor the shape of the workpiece during the deformation in order to ensure the correct ring production. In the present paper a ring rolling process has been studied and optimized in order to obtain anular components to be used in aerospace applications. In particular, the influence of process input parameters (feed rate of the mandrel and angular speed of main roll) on geometrical features of the final ring has been evaluated. For this purpose, a three-dimensional finite element model for HRR (Hot Ring Rolling) has been implemented in SFTC DEFORM V11. The FEM model has been used to formulate a proper optimization problem. The optimization procedure has been implemented in the commercial software DS ISight in order to find the combination of process parameters which allows to minimize the percentage error of each obtained dimension with respect to its nominal value. The software allows to find the relationship between input and output parameters applying Response Surface Methodology (RSM), by using the exact values of output parameters in the control points of the design space explored through FEM simulation. Once this relationship is known, the values of the output parameters can be calculated for each combination of the input parameters. After the calculation of the response surfaces for the selected output parameters, an optimization procedure based on Genetic Algorithms has been applied. At the end, the error between each obtained dimension and its nominal value has been minimized. The constraints imposed were the maximum values of standard deviations of the dimensions obtained for the final ring.

USE OF HIGH SPEED STEEL WORK ROLLS (HSS ON APERAM STECKEL MILL

Directory of Open Access Journals (Sweden)

Arísio de Abreu Barbosa

2013-12-01

Full Text Available This paper outlines the main actions taken to reinforce the decision to use HSS work rolls on the Aperam Steckel Mill. These are: work roll cooling improvements, systematically analyzing Eddy Current and Ultrasonic non destructive tests, mechanical adjustment of work roll crown and critically examining the rolling process. These actions applied together have contributed to the success of HSS rolls state of the art application, and provide the Steckel Mill with a much improved performance. Significant results have been achieved, such as: increasing of work roll change intervals, increasing of the available production time, a yield gain, a product quality improvement, less working hours needed for the roll grinding operation, etc

Rolled-up transformer structure for a radiofrequency integrated circuit (RFIC)

Science.gov (United States)

Li, Xiuling; Huang, Wen

2015-01-27

A rolled-up transformer structure comprises a multilayer sheet having a rolled configuration comprising multiple turns about a longitudinal axis. The multilayer sheet comprises more than one conductive pattern layer on a strain-relieved layer, including a first conductive film and a second conductive film separated from the first conductive film in a thickness direction. The first conductive film comprises an even number of primary conductive strips, where each primary conductive strip has a length extending in the rolling direction, and the second conductive film comprises an even number of secondary conductive strips, where each secondary conductive strip has a length extending in the rolling direction. In the rolled configuration, turns of the primary conductive strips and turns of the secondary conductive strips wrap around the longitudinal axis. The primary conductive strips serve as a primary winding and the secondary conductive strips serve as a secondary winding of the rolled-up transformer structure.

Rolled-up transformer structure for a radiofrequency integrated circuit (RFIC)

Science.gov (United States)

Li, Xiuling; Huang, Wen

2016-05-03

A rolled-up transformer structure comprises a multilayer sheet having a rolled configuration comprising multiple turns about a longitudinal axis. The multilayer sheet comprises more than one conductive pattern layer on a strain-relieved layer, including a first conductive film and a second conductive film separated from the first conductive film in a thickness direction. The first conductive film comprises an even number of primary conductive strips, where each primary conductive strip has a length extending in the rolling direction, and the second conductive film comprises an even number of secondary conductive strips, where each secondary conductive strip has a length extending in the rolling direction. In the rolled configuration, turns of the primary conductive strips and turns of the secondary conductive strips wrap around the longitudinal axis. The primary conductive strips serve as a primary winding and the secondary conductive strips serve as a secondary winding of the rolled-up transformer structure.

I-domain of lymphocyte function-associated antigen-1 mediates rolling of polystyrene particles on ICAM-1 under flow.

Science.gov (United States)

Eniola, A Omolola; Krasik, Ellen F; Smith, Lee A; Song, Gang; Hammer, Daniel A

2005-11-01

In their active state, beta(2)-integrins, such as LFA-1, mediate the firm arrest of leukocytes by binding intercellular adhesion molecules (ICAMs) expressed on endothelium. Although the primary function of LFA-1 is assumed to be the ability to mediate firm adhesion, recent work has shown that LFA-1 can contribute to cell tethering and rolling under hydrodynamic flow, a role previously largely attributed to the selectins. The inserted (I) domain of LFA-1 has recently been crystallized in the wild-type (wt) and locked-open conformations and has been shown to, respectively, support rolling and firm adhesion under flow when expressed in alpha(L)beta(2) heterodimers or as isolated domains on cells. Here, we report results from cell-free adhesion assays where wt I-domain-coated polystyrene particles were allowed to interact with ICAM-1-coated surfaces in shear flow. We show that wt I-domain can independently mediate the capture of particles from flow and support their rolling on ICAM-1 surfaces in a manner similar to how carbohydrate-selectin interactions mediate rolling. Adhesion is specific and blocked by appropriate antibodies. We also show that the rolling velocity of I-domain-coated particles depends on the wall shear stress in flow chamber, I-domain site density on microsphere surfaces, and ICAM-1 site density on substrate surfaces. Furthermore, we show that rolling is less sensitive to wall shear stress and ICAM-1 substrate density at high density of I-domain on the microsphere surface. Computer simulations using adhesive dynamics can recreate bead rolling dynamics and show that the mechanochemical properties of ICAM-1-I-domain interactions are similar to those of carbohydrate-selectin interactions. Understanding the biophysics of adhesion mediated by the I-domain of LFA-1 can elucidate the complex roles this integrin plays in leukocyte adhesion in inflammation.

Silver front electrode grids for ITO-free all printed polymer solar cells with embedded and raised topographies, prepared by thermal imprint, flexographic and inkjet roll-to-roll processes.

Science.gov (United States)

Yu, Jong-Su; Kim, Inyoung; Kim, Jung-Su; Jo, Jeongdai; Larsen-Olsen, Thue T; Søndergaard, Roar R; Hösel, Markus; Angmo, Dechan; Jørgensen, Mikkel; Krebs, Frederik C

2012-09-28

Semitransparent front electrodes for polymer solar cells, that are printable and roll-to-roll processable under ambient conditions using different approaches, are explored in this report. The excellent smoothness of indium-tin-oxide (ITO) electrodes has traditionally been believed to be difficult to achieve using printed front grids, as surface topographies accumulate when processing subsequent layers, leading to shunts between the top and bottom printed metallic electrodes. Here we demonstrate how aqueous nanoparticle based silver inks can be employed as printed front electrodes using several different roll-to-roll techniques. We thus compare hexagonal silver grids prepared using either roll-to-roll inkjet or roll-to-roll flexographic printing. Both inkjet and flexo grids present a raised topography and were found to perform differently due to only the conductivity of the obtained silver grid. The raised topographies were compared with a roll-to-roll thermally imprinted grid that was filled with silver in a roll-to-roll process, thus presenting an embedded topography. The embedded grid and the flexo grid were found to perform equally well, with the flexographic technique currently presenting the fastest processing and the lowest silver use, whereas the embedded grid presents the maximally achievable optical transparency and conductivity. Polymer solar cells were prepared in the same step, using roll-to-roll slot-die coating of zinc oxide as the electron transport layer, poly-3-hexylthiophene:phenyl-C(61)-butyric acid methyl ester (P3HT:PCBM) as the active layer and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) as the top electrode, along with a flat bed screen printed silver grid. The power conversion efficiency (PCE) obtained for large area devices (6 cm(2)) was 1.84%, 0.79% and 1.72%, respectively, for thermally imprinted, inkjet and flexographic silver grids, tested outside under the real sun. Central to all three approaches was that they

Optical scattering from rough-rolled aluminum surfaces.

Science.gov (United States)

Rönnelid, M; Adsten, M; Lindström, T; Nostell, P; Wäckelgård, E

2001-05-01

Bidirectional, angular resolved scatterometry was used to evaluate the feasibility of using rolled aluminum as reflectors in solar thermal collectors and solar cells. Two types of rolled aluminum with different surface roughnesses were investigated. The results show that the smoother of the two samples [rms height, (0.20 ? 0.02) mum] can be used as a nonimaging, concentrating reflector with moderate reflection losses compared with those of optically smooth aluminum reflectors. The sample with the rougher surface [rms height, (0.6 ? 0.1) mum] is not suitable as a concentrating element but can be used as planar reflectors. The orientation of the rolling grooves is then of importance for minimizing reflection losses in the system.

Influence of Surge on Extreme Roll Amplitudes

DEFF Research Database (Denmark)

Vidic-Perunovic, Jelena; Rognebakke, Olav; Pedersen, Preben Terndrup

2008-01-01

Interference of the wave-induced ship surge motion with roll dynamics has been studied. The surge motion has been included in a previously derived hydrodynamic roll prediction model in order to account for the ship speed variation due to the longitudinal incident wave pressure force. Depending...... balanced in order to determine the added thrust term that would represent actions to maintain speed The resulting forward speed variation affects the frequency of encounter and the parametric roll resonant condition is directly influenced by this speed variation. The analysis procedure is demonstrated...... for an example containership sailing mainly in head sea condition and higher sea states. Sensitivity of the results to the added thrust model and vertical motion calculation is discussed....

Robust Rudder Roll Damping Control

DEFF Research Database (Denmark)

Yang, C.

The results of a systematic research to solve a specific ship motion control problem, simultaneous roll damping and course keeping using the rudder are presented in this thesis. The fundamental knowledge a priori is that rudder roll damping is highly sensitive to the model uncertainty, therefore H-infinity...... theory is used to deal with the problem. The necessary mathematical tools and the H-Infinity theory as the basis of controller design are presented in Chapter 2 and 3. The mu synthesis and the D-K iteration are introduced in Chapter 3. The ship dynamics and modeling technology are discussed in Chapter 4...

Assessment of friction between a rolling cylindrical element and a ...

African Journals Online (AJOL)

A cost-effective friction coefficient measuring technique was developed and tested. The technique involved the mounting of two sensing elements on the surface of rolls, in a manner that sought to measure simultaneously the normal and the tangential stresses during rolling. The instrumented roll termed “SGRoll” was ...

Measurement by a cylinder test stand and tyre rolling resistance

Directory of Open Access Journals (Sweden)

A. Dávid

2006-03-01

Full Text Available Sometimes it is necessary to test how repair affects the properties of the car. These tests are carried out using a cylinder test stand. During the test the tyre is rolling between two cylinders of a small diameter. The question arises whether the rolling resistance of the tyre is the same as the rolling resistance when the wheel is rolling on the plane. If it is not the same what is the reliation between tyre resistances in these two cases? It is an important answer because the change of rolling resistance can affect consumption, the highest speed, engine power and other results of measurement. The paper gives the answer to these questions and describes the method of getting this information.

Pre-treatment of Biomass By Rolling - A Combined Experimental and Numerical Analysis

DEFF Research Database (Denmark)

Hansen, Klaus Schütt; Ravn, Christian; Nielsen, Emil Krabbe

2017-01-01

Pre-treatment of bulk straw material by rolling is studied as a possible method to prepare for subsequent biogas production. A combined experimental and theoretical study is presented. A pilot rolling mill with a double screw feeder is designed and constructed for crushing of bulk straw. Experime...... process window for pre-treatment of wheat straw by roll pressing varying the feed, the roll gap, the roll speed and the moisture content of the bulk straw.......Pre-treatment of bulk straw material by rolling is studied as a possible method to prepare for subsequent biogas production. A combined experimental and theoretical study is presented. A pilot rolling mill with a double screw feeder is designed and constructed for crushing of bulk straw....... Experiments show that the roll speed and the roll reduction should be chosen within a specific range depending on the injection screw speed to avoid blocking or insufficient compaction. A mechanical testing procedure of the bulk straw material including closed die compaction testing as well as simple...

Biografija : [luuletused] / Viivi Luik ; tlk. Svetlan Semenenko, lühibiograafia: T. L. [Toomas Liiv

Index Scriptorium Estoniae

Luik, Viivi, 1946-

1999-01-01

Sisu: Biografija ; "Smeisja, smeisja, davai smejatsja..." ; Rodnoi dom / tlk. Nora Javorskaja ; Molba ; "Ja skazhu: kol ne ja..." ; Starõi gorod ; V dorogu ; "Togo uzh ne naiti, kto zaplutal v meteli..." ; Ona skazala vsjo ; Bolshije doma ; Snova ; "Neprestanno krov vo mne zhurtshit..." / tlk. Svetlan Semenenko. Orig.: Elulugu ; "Naera, naera, naerame võidu..." ; Kodu ; Palve ; "Mõtlen: kui mitte mina...", "Ei jäta jälgi see, kes eksis sadudesse..." ; Ta on kõik öelnud ; Uued suured majad ; Jälle

Microstructure and properties of hot roll bonding layer of dissimilar metals. 2. Bonding interface microstructure of Zr/stainless steel by hot roll bonding and its controlling

International Nuclear Information System (INIS)

Yasuyama, Masanori; Ogawa, Kazuhiro; Taka, Takao; Nakasuji, Kazuyuki; Nakao, Yoshikuni; Nishimoto, Kazutoshi.

1996-01-01

The hot roll bonding of zirconium and stainless steel inserted with tantalium was investigated using the newly developed rolling mill. The effect of hot rolling temperatures of zirconium/stainless steel joints on bonding interface structure was evaluated. Intermetallic compound layer containing cracks was observed at the bonding interface between stainless steel and tantalium when the rolling temperature was above 1373K. The hardness of the bonding layer of zirconium and tantalium bonded above 1273K was higher than tantalium or zirconium base metal in spite of absence of intermetallic compound. The growth of reaction layer at the stainless steel and tantalium interface and at the tantalium and zirconium interface was conforming a parabolic low when that was isothermally heated after hot roll bonding, and the growth rate was almost same as that of static diffusion bonding without using hot roll bonding process. It is estimated that the strain caused by hot roll bonding gives no effect on the growth of reaction layer. It was confirmed that the dissimilar joint of zirconium and stainless steel with insert of tantalium having the sound bonding interface were obtained at the suitable bonding temperature of 1173K by the usage of the newly developed hot roll bonding process. (author)

Person og Rolle

DEFF Research Database (Denmark)

Szatkowski, Janek

2011-01-01

Distinktionen mellem person og rolle forslås som grundlag for et præcist og anlytisk anvendeligt begreb om performativitet. Begrebet tager sigte på at beskrive enkeltindividers og gruppers kommunikation med henblik på hvordan kommunikation etableres. Performativitet gør det muligt at iagttage den...

MicroRNA-10b downregulation mediates acute rejection of renal allografts by derepressing BCL2L11

Energy Technology Data Exchange (ETDEWEB)

Liu, Xiaoyou [Department of Organ Transplantation, Zhujiang Hospital, Guangzhou 510282 (China); Dong, Changgui [Institute of Molecular Ecology and Evolution, East China Normal University, Shanghai 200062 (China); Jiang, Zhengyao [Department of Organ Transplantation, Zhujiang Hospital, Guangzhou 510282 (China); Wu, William K.K. [Department of Anaesthesia and Intensive Care, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); State Key Laboratory of Digestive Diseases, LKS Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); Chan, Matthew T.V. [Department of Anaesthesia and Intensive Care, The Chinese University of Hong Kong, Shatin, NT, Hong Kong (China); Zhang, Jie [Department of Organ Transplantation, Zhujiang Hospital, Guangzhou 510282 (China); Li, Haibin; Qin, Ke [Guangxi Key Laboratory for Transplantation Medicine Department of Organ Transplantation in Guangzhou Military Region, Institute of Transplant Medicine, 303 Hospital of People' s Liberation Army, Nanning, Guangxi 530021 (China); Sun, Xuyong, E-mail: sunxuyong0528@163.com [Guangxi Key Laboratory for Transplantation Medicine Department of Organ Transplantation in Guangzhou Military Region, Institute of Transplant Medicine, 303 Hospital of People' s Liberation Army, Nanning, Guangxi 530021 (China)

2015-04-10

Kidney transplantation is the major therapeutic option for end-stage kidney diseases. However, acute rejection could cause allograft loss in some of these patients. Emerging evidence supports that microRNA (miRNA) dysregulation is implicated in acute allograft rejection. In this study, we used next-generation sequencing to profile miRNA expression in normal and acutely rejected kidney allografts. Among 75 identified dysregulated miRNAs, miR-10b was the most significantly downregulated miRNAs in rejected allografts. Transfecting miR-10b inhibitor into human renal glomerular endothelial cells recapitulated key features of acute allograft rejection, including endothelial cell apoptosis, release of pro-inflammatory cytokines (interleukin-6, tumor necrosis factor α, interferon-γ, and chemokine (C–C motif) ligand 2) and chemotaxis of macrophages whereas transfection of miR-10b mimics had opposite effects. Downregulation of miR-10b directly derepressed the expression of BCL2L11 (an apoptosis inducer) as revealed by luciferase reporter assay. Taken together, miR-10b downregulation mediates many aspects of disease pathogenicity of acute kidney allograft rejection. Restoring miR-10b expression in glomerular endothelial cells could be a novel therapeutic approach to reduce acute renal allograft loss. - Highlights: • miR-10b was the most downregulated microRNAs in acutely rejected renal allografts. • miR-10b downregulation triggered glomerular endothelial cell apoptosis. • miR-10b downregulation induced release of pro-inflammatory cytokines. • miR-10b downregulation derepressed its pro-apoptotic target BCL2L11.

MicroRNA-10b downregulation mediates acute rejection of renal allografts by derepressing BCL2L11

International Nuclear Information System (INIS)

Liu, Xiaoyou; Dong, Changgui; Jiang, Zhengyao; Wu, William K.K.; Chan, Matthew T.V.; Zhang, Jie; Li, Haibin; Qin, Ke; Sun, Xuyong

2015-01-01

Kidney transplantation is the major therapeutic option for end-stage kidney diseases. However, acute rejection could cause allograft loss in some of these patients. Emerging evidence supports that microRNA (miRNA) dysregulation is implicated in acute allograft rejection. In this study, we used next-generation sequencing to profile miRNA expression in normal and acutely rejected kidney allografts. Among 75 identified dysregulated miRNAs, miR-10b was the most significantly downregulated miRNAs in rejected allografts. Transfecting miR-10b inhibitor into human renal glomerular endothelial cells recapitulated key features of acute allograft rejection, including endothelial cell apoptosis, release of pro-inflammatory cytokines (interleukin-6, tumor necrosis factor α, interferon-γ, and chemokine (C–C motif) ligand 2) and chemotaxis of macrophages whereas transfection of miR-10b mimics had opposite effects. Downregulation of miR-10b directly derepressed the expression of BCL2L11 (an apoptosis inducer) as revealed by luciferase reporter assay. Taken together, miR-10b downregulation mediates many aspects of disease pathogenicity of acute kidney allograft rejection. Restoring miR-10b expression in glomerular endothelial cells could be a novel therapeutic approach to reduce acute renal allograft loss. - Highlights: • miR-10b was the most downregulated microRNAs in acutely rejected renal allografts. • miR-10b downregulation triggered glomerular endothelial cell apoptosis. • miR-10b downregulation induced release of pro-inflammatory cytokines. • miR-10b downregulation derepressed its pro-apoptotic target BCL2L11

Low Band Gap Polymers for Roll-to-Roll Coated Polymer Solar Cells

DEFF Research Database (Denmark)

Bundgaard, Eva; Hagemann, Ole; Manceau, Matthieu

2010-01-01

connected cells were prepared with a total module active area of 96 cm2. The devices were tested for operational stability under simulated sunlight (AM1.5G) and natural sunlight, and the photochemical stability of the polymer was examined using a combination of UV−vis and IR spectroscopy.......We present the synthesis of a low band gap copolymer based on dithienothiophene and dialkoxybenzothiadiazole (poly(dithienothiophene-co-dialkoxybenzothiadiazole), PDTTDABT). The optical properties of the polymer showed a band gap of 1.6 eV and a sky-blue color in solid films. The polymer...... around a 1:2 mixing ratio. Roll-to-roll coated polymer solar cell devices were prepared under ambient conditions employing solution processing in all steps including the metallic back electrode that was printed as a grid giving semitransparent solar cell devices. Solar cell modules comprising 16 serially...

MicroRNA-340 inhibits the proliferation and promotes the apoptosis of colon cancer cells by modulating REV3L

Science.gov (United States)

Arivazhagan, Roshini; Lee, Jaesuk; Bayarsaikhan, Delger; Kwak, Peter; Son, Myeongjoo; Byun, Kyunghee; Salekdeh, Ghasem Hosseini; Lee, Bonghee

2018-01-01

DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) has recently emerged as an important oncogene. Although the expressions of REV3L are similar in normal and cancer cells, several mutations in REV3L have been shown to play important roles in cancer. These mutations cause proteins misfolding and mislocalization, which in turn alters their interactions and biological functions. miRNAs play important regulatory roles during the progression and metastasis of several human cancers. This study was undertaken to determine how changes in the location and interactions of REV3L regulate colon cancer progression. REV3L protein mislocalization confirmed from the immunostaining results and the known interactions of REV3L was found to be broken as seen from the PLA assay results. The mislocalized REV3L might interact with new proteins partners in the cytoplasm which in turn may play role in regulating colon cancer progression. hsa-miR-340 (miR-340), a microRNA down-regulated in colon cancer, was used to bind to and downregulate REV3L, and found to control the proliferation and induce the apoptosis of colon cancer cells (HCT-116 and DLD-1) via the MAPK pathway. Furthermore, this down-regulation of REV3L also diminished colon cancer cell migration, and down-regulated MMP-2 and MMP-9. Combined treatment of colon cancer cells with miR-340 and 5-FU enhanced the inhibitory effects of 5-FU. In addition, in vivo experiments conducted on nude mice revealed tumor sizes were smaller in a HCT-116-miR-340 injected group than in a HCT-116-pCMV injected group. Our findings suggest mutations in REV3L causes protein mislocalization to the cytoplasm, breaking its interaction and is believed to form new protein interactions in cytoplasm contributing to colon cancer progression. Accordingly, microRNA-340 appears to be a good candidate for colon cancer therapy. PMID:29435169

GoQBot: a caterpillar-inspired soft-bodied rolling robot

International Nuclear Information System (INIS)

Lin, Huai-Ti; Trimmer, Barry; Leisk, Gary G

2011-01-01

Rolling locomotion using an external force such as gravity has evolved many times. However, some caterpillars can curl into a wheel and generate their own rolling momentum as part of an escape repertoire. This change in body conformation occurs well within 100 ms and generates a linear velocity over 0.2 m s -1 , making it one of the fastest self-propelled wheeling behaviors in nature. Inspired by this behavior, we construct a soft-bodied robot to explore the dynamics and control issues of ballistic rolling. This robot, called GoQBot, closely mimics caterpillar rolling. Analyzing the whole body kinematics and 2D ground reaction forces at the robot ground anchor reveals about 1G of acceleration and more than 200 rpm of angular velocity. As a novel rolling robot, GoQBot demonstrates how morphing can produce new modes of locomotion. Furthermore, mechanical coupling of the actuators improves body coordination without sensory feedback. Such coupling is intrinsic to soft-bodied animals because there are no joints to isolate muscle-generated movements. Finally, GoQBot provides an estimate of the mechanical power for caterpillar rolling that is comparable to that of a locust jump. How caterpillar musculature produces such power in such a short time is yet to be discovered.

GoQBot: a caterpillar-inspired soft-bodied rolling robot

Energy Technology Data Exchange (ETDEWEB)

Lin, Huai-Ti; Trimmer, Barry [Department of Biology, Tufts University, 163 Packard Avenue, Medford, MA 02155 (United States); Leisk, Gary G, E-mail: huaiti.lin@gmail.com, E-mail: gary.leisk@tufts.edu, E-mail: barry.trimmer@tufts.edu [Department of Mechanical Engineering, Tufts University, 200 College Avenue, Medford, MA 02155 (United States)

2011-06-15

Rolling locomotion using an external force such as gravity has evolved many times. However, some caterpillars can curl into a wheel and generate their own rolling momentum as part of an escape repertoire. This change in body conformation occurs well within 100 ms and generates a linear velocity over 0.2 m s{sup -1}, making it one of the fastest self-propelled wheeling behaviors in nature. Inspired by this behavior, we construct a soft-bodied robot to explore the dynamics and control issues of ballistic rolling. This robot, called GoQBot, closely mimics caterpillar rolling. Analyzing the whole body kinematics and 2D ground reaction forces at the robot ground anchor reveals about 1G of acceleration and more than 200 rpm of angular velocity. As a novel rolling robot, GoQBot demonstrates how morphing can produce new modes of locomotion. Furthermore, mechanical coupling of the actuators improves body coordination without sensory feedback. Such coupling is intrinsic to soft-bodied animals because there are no joints to isolate muscle-generated movements. Finally, GoQBot provides an estimate of the mechanical power for caterpillar rolling that is comparable to that of a locust jump. How caterpillar musculature produces such power in such a short time is yet to be discovered.

The advantages of a rolling foot in human walking.

Science.gov (United States)

Adamczyk, Peter G; Collins, Steven H; Kuo, Arthur D

2006-10-01

The plantigrade human foot rolls over the ground during each walking step, roughly analogous to a wheel. The center of pressure progresses on the ground like a wheel of radius 0.3 L (leg length). We examined the effect of varying foot curvature on the mechanics and energetics of walking. We controlled curvature by attaching rigid arc shapes of various radii to the bottoms of rigid boots restricting ankle motion. We measured mechanical work performed on the center of mass (COM), and net metabolic rate, in human subjects (N=10) walking with seven arc radii from 0.02-0.40 m. Simple models of dynamic walking predict that redirection of COM velocity requires step-to-step transition work, decreasing quadratically with arc radius. Metabolic cost would be expected to change in proportion to mechanical work. We measured the average rate of negative work performed on the COM, and found that it followed the trend well (r2=0.95), with 2.37 times as much work for small radii as for large. Net metabolic rate (subtracting quiet standing) also decreased with increasing arc radius to a minimum at 0.3 L, with a slight increase thereafter. Maximum net metabolic rate was 6.25 W kg(-1) (for small-radius arc feet), about 59% greater than the minimum rate of 3.93 W kg(-1), which in turn was about 45% greater than the rate in normal walking. Metabolic rate was fit reasonably well (r2=0.86) by a quadratic curve, but exceeded that expected from COM work for extreme arc sizes. Other factors appear to increase metabolic cost for walking on very small and very large arc feet. These factors may include effort expended to stabilize the joints (especially the knee) or to maintain balance. Rolling feet with curvature 0.3 L appear energetically advantageous for plantigrade walking, partially due to decreased work for step-to-step transitions.

Precision ring rolling technique and application in high-performance bearing manufacturing

Directory of Open Access Journals (Sweden)

Hua Lin

2015-01-01

Full Text Available High-performance bearing has significant application in many important industry fields, like automobile, precision machine tool, wind power, etc. Precision ring rolling is an advanced rotary forming technique to manufacture high-performance seamless bearing ring thus can improve the working life of bearing. In this paper, three kinds of precision ring rolling techniques adapt to different dimensional ranges of bearings are introduced, which are cold ring rolling for small-scale bearing, hot radial ring rolling for medium-scale bearing and hot radial-axial ring rolling for large-scale bearing. The forming principles, technological features and forming equipments for three kinds of precision ring rolling techniques are summarized, the technological development and industrial application in China are introduced, and the main technological development trend is described.

Bearing selection in ball-rolling dung beetles: is it constant?

Science.gov (United States)

Baird, Emily; Byrne, Marcus J; Scholtz, Clarke H; Warrant, Eric J; Dacke, Marie

2010-11-01

Ball rolling in dung beetles is thought to have evolved as a means to escape intense inter- and intra-specific competition at the dung pile. Accordingly, dung beetles typically roll along a straight-line path away from the pile, this being the most effective escape strategy for transporting dung to a suitable burial site. In this study, we investigate how individual diurnal dung beetles, Scarabaeus (Kheper) nigroaeneus, select the compass bearing of their straight-line rolls. In particular, we examine whether roll bearings are constant with respect to geographic cues, celestial cues, or other environmental cues (such as wind direction). Our results reveal that the roll bearings taken by individual beetles are not constant with respect to geographic or celestial references. Environmental cues appear to have some influence over bearing selection, although the relationship is not strong. Furthermore, the variance in roll bearing that we observe is not affected by the presence or absence of other beetles. Thus, rather than being constant for individual beetles, bearing selection varies each time a beetle makes a ball and rolls it away from the dung pile. This strategy allows beetles to make an efficient escape from the dung pile while minimizing the chance of encountering competition.

Implication of human handling on packaged sausage rolls during sale

Directory of Open Access Journals (Sweden)

Coolborn AKHARAIYI FRED

2016-08-01

Full Text Available In other to identify the implication of human handling of packaged sausage rolls after production, a microbiological safety evaluation was carried out on sausage rolls sold on street and in shops. Among the sausage rolls, gala purchased from street vendors has the highest bacterial load of 2.82 × 104 CFU/g and 4.3 × 106 spore/g of fungal load, followed by meaty with bacterial load of 1.71 × 104 CFU/g and fungal load of 1.6 × 105 spore/g and was least in rite sausage roll with 1.46 × 104 CFU/g and 1 × 105 spore/g bacterial and fungal loads respectively. Seventeen bacteria species were isolated from both street vended and shop sold sausage rolls, the isolates identified including: Bacillus cereus, Acinetobacter calcoaceticus, Alcaligenes faecalis, Citrobacter freundii, Klebsiella ozaenae, Staphylococcus epidermidis, Enterobacter aerogenes, Staphylococcus aureus, Aeromonas hydrophila, Plesiomonas shigelloides, Moraxella catarhalis, Bacillus substilis, Escherichia coli, Salmonella typhi, Aeromonas anaerogenes, Aerococcus viridans and Azomonas agilis. Five fungi species were isolated from street vended sausage rolls only. The fungal species are Penicillium notatum, Aspergillus parasiticus, Aspergillus flavus, Penicillium italicum and Gliocephalis spp. From this study, street vended samples have higher microbial contamination than shop sold sausage rolls due to improper handling during sales.

77 FR 6668 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

Science.gov (United States)

2012-02-09

... Airworthiness Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT... all Rolls-Royce plc RB211-Trent 500 series turbofan engines. This AD requires a one-time inspection of... RB211- Trent 560A2-61 turbofan engines that have not complied with Rolls- Royce plc Service Bulletin No...

Deformation mechanisms of pure Mg materials fabricated by using pre-rolled powders

Energy Technology Data Exchange (ETDEWEB)

Shen, J., E-mail: shen-j@jwri.osaka-u.ac.jp [Joining and Welding Research Institute, Osaka University (Japan); Imai, H. [Joining and Welding Research Institute, Osaka University (Japan); Chen, B. [Graduate School of Engineering, Osaka University (Japan); Ye, X.; Umeda, J.; Kondoh, K. [Joining and Welding Research Institute, Osaka University (Japan)

2016-03-21

In the present work, a powder rolling process was utilized in the fabrication of fine grained pure Mg via powder metallurgy. Mg flakes were obtained after each rolling process, and broken into pieces for further rolling or sintering. Mg samples of experiencing 0, 5 and 10 rolling passes were obtained following spark plasma sintering (SPS) and hot extrusion. Microstructural results from electron backscatter diffraction (EBSD) revealed that, without experiencing powder rolling, the specimen contained a great number of residual tensile twins; in contrast, after powder rolling the specimen showed uniform and equiaxed grain structures. In addition, the average grain size was measured to be around 9.2, 2.9 and 2.1 µm for the samples subjected to 0, 5 and 10 rolling passes. The powder rolled specimens were found superior in mechanical properties. Post-loading microstructure examinations were also performed for the samples and a discussion regarding the relationship between their mechanical behavior and microstructures was provided.

Deformation mechanisms of pure Mg materials fabricated by using pre-rolled powders

International Nuclear Information System (INIS)

Shen, J.; Imai, H.; Chen, B.; Ye, X.; Umeda, J.; Kondoh, K.

2016-01-01

In the present work, a powder rolling process was utilized in the fabrication of fine grained pure Mg via powder metallurgy. Mg flakes were obtained after each rolling process, and broken into pieces for further rolling or sintering. Mg samples of experiencing 0, 5 and 10 rolling passes were obtained following spark plasma sintering (SPS) and hot extrusion. Microstructural results from electron backscatter diffraction (EBSD) revealed that, without experiencing powder rolling, the specimen contained a great number of residual tensile twins; in contrast, after powder rolling the specimen showed uniform and equiaxed grain structures. In addition, the average grain size was measured to be around 9.2, 2.9 and 2.1 µm for the samples subjected to 0, 5 and 10 rolling passes. The powder rolled specimens were found superior in mechanical properties. Post-loading microstructure examinations were also performed for the samples and a discussion regarding the relationship between their mechanical behavior and microstructures was provided.

Energy-based control for a biologically inspired hexapod robot with rolling locomotion

Directory of Open Access Journals (Sweden)

Takuma Nemoto

2015-04-01

Full Text Available This paper presents an approach to control rolling locomotion on the level ground with a biologically inspired hexapod robot. For controlling rolling locomotion, a controller which can compensate energy loss with rolling locomotion of the hexapod robot is designed based on its dynamic model. The dynamic model describes the rolling locomotion which is limited to planar one by an assumption that the hexapod robot does not fall down while rolling and influences due to collision and contact with the ground, and it is applied for computing the mechanical energy of the hexapod robot and a plant for a numerical simulation. The numerical simulation of the rolling locomotion on the level ground verifies the effectiveness of the proposed controller. The simulation results show that the hexapod robot can perform the rolling locomotion with the proposed controller. In conclusion, it is shown that the proposed control approach is effective in achieving the rolling locomotion on the level ground.

Roll-to-Roll Printed Silver Nanowire Semitransparent Electrodes for Fully Ambient Solution-Processed Tandem Polymer Solar Cells

DEFF Research Database (Denmark)

Angmo, Dechan; Andersen, Thomas Rieks; Bentzen, Janet Jonna

2015-01-01

Silver nanowires (AgNWs) and zinc oxide (ZnO) are deposited on flexible substrates using fast roll-to-roll (R2R) processing. The AgNW film on polyethylene terephthalate (PET) shows >80% uniform optical transmission in the range of 550-900 nm. This electrode is compared to the previously reported...... spectrum reaching up to 40% increased transmission at 750 nm in comparison to Flextrode. The functionality of AgNW electrodes is demonstrated in single and tandem polymer solar cells and compared with parallel devices on traditional Flextrode. All layers, apart from the semitransparent electrodes which...... are large-scale R2R produced, are fabricated in ambient conditions on a laboratory roll-coater using printing and coating methods which are directly transferrable to large-scale R2R processing upon availability of materials. In a single cell structure, Flextrode is preferable with active layers based...

MODELING OF THE TRACK AND ROLLING STOCK INTERACTION

Directory of Open Access Journals (Sweden)

N. V. Khalipova

2013-09-01

Full Text Available Purpose. Interaction of system’s elements of "carriage–track" modelling requires consideration of various criteria, it also requires analysis of many uncertainty and randomness factors’ influence on the basic parameters to ensure optimal or rational parameters of the system. The researching of interactions’ process requires new theoretical approaches to formulation of objectives, based on a generalization of existing modeling approaches. The purpose of this work is development of interaction models between track and rolling stock based on multiple structures of objects. Methodology. Dedicated and formed the main evaluation criteria of dynamic interaction between track and rolling stock optimization - quality assurance and safety of transportation process, improving of their efficiency and reducing of prime cost’s. Based on vector optimization methods, proposed model of rolling stock and track’s elements interaction. For the synthesis of the model used mathematical machine of multiple objects structures. Findings. Generalized approaches to modeling in the interaction of rolling stock and track for different structural elements of the system under different exploitation conditions. This theoretical approach demonstrated on the examples of modeling of passenger and freight cars with track under different exploitation conditions. Originality. Proposed theoretical approach to the problem of track and rolling stock interaction, based on a synthesis of existing models by using of multiple objects structures. Practical value. Using of proposed model allows to structure key data and rational parameters of rolling stock and track interaction’s modeling and to formulate optimal and rational parameters of the system, to determine the effective exploitation parameters and measurement system for rational use of infrastructure.

Product integration of compact roll-to-roll processed polymer solar cell modules: methods and manufacture using flexographic printing, slot-die coating and rotary screen printing

DEFF Research Database (Denmark)

Krebs, Frederik C; Fyenbo, Jan; Jørgensen, Mikkel

2010-01-01

The improvement of the performance of roll-to-roll processed polymer solar cell modules through miniaturization of the device outline is described. The devices were prepared using full roll-to-roll processing comprising flexographic printing, slot-die coating and rotary screen printing to create ......HT:[70]PCBM. The solar cell modules were used to demonstrate the complete manufacture of a small lamp entirely using techniques of flexible electronics. The solar cell module was used to charge a polymer lithium ion battery through a blocking diode. The entire process was fully automated...

Texture and superelastic behavior of cold-rolled TiNbTaZr alloy

International Nuclear Information System (INIS)

Wang Liqiang; Lu Weijie; Qin Jining; Zhang Fan; Zhang Di

2008-01-01

This work investigates the deformation texture and strain-induced α'' martensite texture of TiNbTaZr alloy during cold rolling. The alloy is rolled by 20% and 90% reductions without changing rolling direction. Textures of cold-rolled specimens are investigated by X-ray diffraction measurements. Besides {2 2 1} β β twinning texture, {1 0 0} β β texture is developed in the specimen with 20% reduction. In the 90% cold-rolled specimen, {1 0 0} β β texture appears along rolling direction and strain-induced α'' martensite texture tends to [0 1 0] and [0 0 1] directions along rolling direction (RD) and transverse direction (TD), respectively. Superelastic strain (ε SE ) exhibits higher value along RD and TD. Pure elastic strain (ε E ) shows higher value along RD and 45 deg. from RD

L-DOPA decarboxylase mRNA expression is associated with tumor stage and size in head and neck squamous cell carcinoma: a retrospective cohort study

Directory of Open Access Journals (Sweden)

Geomela Panagiota-Aikaterini

2012-10-01

Full Text Available Abstract Background Head and neck squamous cell carcinoma (HNSCC represents one of the most commonly diagnosed malignancies worldwide. The DDC gene encodes L-DOPA decarboxylase, an enzyme catalyzing the decarboxylation of L-DOPA to dopamine. We have recently shown that DDC mRNA is a significant predictor of patients’ prognosis in colorectal adenocarcinoma and prostate cancer. The aim of the current study was to analyze the DDC mRNA expression in HNSCC patients. Methods 53 malignant tumors were resected from the larynx, pharynx, tongue, buccal mucosa, parotid glands, and nasal cavity, as well as from 34 adjacent non-cancerous tissues of HNSCC patients, and were homogenized. Total RNA was isolated and converted into first-strand cDNA. An ultrasensitive real-time PCR method based on the SYBR Green chemistry was used for DDC mRNA quantification in head and neck tissue specimens. Relative quantification was performed using the comparative Ct (2-ddCt method. Results DDC mRNA levels were lower in squamous cell carcinomas (SCCs of the larynx and tongue than in adjacent non-cancerous tissue specimens. Furthermore, low DDC mRNA expression was noticed in laryngeal and tongue tumors of advanced TNM stage or bigger size, compared to early-stage or smaller tumors, respectively. No statistically significant differences were observed between SCCs resected from pharynx, buccal mucosa, or nasal cavity, and their normal counterparts. Conclusion This is the first study examining the DDC mRNA expression in HNSCC. According to our results, DDC mRNA expression may constitute a potential prognostic biomarker in tongue and/or larynx SCCs, which principally represent the overwhelming majority of HNSCC cases.

L-DOPA decarboxylase mRNA expression is associated with tumor stage and size in head and neck squamous cell carcinoma: a retrospective cohort study

International Nuclear Information System (INIS)

Geomela, Panagiota-Aikaterini; Kontos, Christos K; Yiotakis, Ioannis; Fragoulis, Emmanuel G; Scorilas, Andreas

2012-01-01

Head and neck squamous cell carcinoma (HNSCC) represents one of the most commonly diagnosed malignancies worldwide. The DDC gene encodes L-DOPA decarboxylase, an enzyme catalyzing the decarboxylation of L-DOPA to dopamine. We have recently shown that DDC mRNA is a significant predictor of patients’ prognosis in colorectal adenocarcinoma and prostate cancer. The aim of the current study was to analyze the DDC mRNA expression in HNSCC patients. 53 malignant tumors were resected from the larynx, pharynx, tongue, buccal mucosa, parotid glands, and nasal cavity, as well as from 34 adjacent non-cancerous tissues of HNSCC patients, and were homogenized. Total RNA was isolated and converted into first-strand cDNA. An ultrasensitive real-time PCR method based on the SYBR Green chemistry was used for DDC mRNA quantification in head and neck tissue specimens. Relative quantification was performed using the comparative Ct (2 -ddCt ) method. DDC mRNA levels were lower in squamous cell carcinomas (SCCs) of the larynx and tongue than in adjacent non-cancerous tissue specimens. Furthermore, low DDC mRNA expression was noticed in laryngeal and tongue tumors of advanced TNM stage or bigger size, compared to early-stage or smaller tumors, respectively. No statistically significant differences were observed between SCCs resected from pharynx, buccal mucosa, or nasal cavity, and their normal counterparts. This is the first study examining the DDC mRNA expression in HNSCC. According to our results, DDC mRNA expression may constitute a potential prognostic biomarker in tongue and/or larynx SCCs, which principally represent the overwhelming majority of HNSCC cases

Roll-to-Roll Inkjet Printing and Photonic Sintering of Electrodes for ITO Free Polymer Solar Cell Modules and Facile Product Integration

DEFF Research Database (Denmark)

Angmo, Dechan; Larsen-Olsen, Thue Trofod; Jørgensen, Mikkel

2013-01-01

Small polymer solar cell modules that are manufactured without indium-tin-oxide using only roll-to-roll printing and coating techniques under ambient conditions enable facile integration into a simple demonstrator (for example a laser pointer). Semitransparent front electrode grid structures prep...

Experimental analysis of two-layered dissimilar metals by roll bonding

Science.gov (United States)

Zhao, Guanghui; Li, Yugui; Li, Juan; Huang, Qingxue; Ma, Lifeng

2018-02-01

Rolling reduction and base layers thickness have important implications for rolling compounding. A two-layered 304 stainless steel/Q345R low alloyed steel was roll bonded. The roll bonding was performed at the three thickness reductions of 25%, 40% and 55% with base layers of various thicknesses (Q345R). The microstructures of the composite were investigated by the ultra-deep microscope (OM) and scanning electron microscope (SEM) and Transmission electron microscope (TEM). Simultaneously, the mechanical properties of the composite were experimentally measured and the tensile fracture surfaces were observed by SEM. The interfaces were successfully bonded without any cracking or voids, which indicated a good fabrication of the 304/Q345R composite. The rolling reduction rate and thinning increase of the substrate contributed to the bonding effects appearance of the roll bonded sheet. The Cr and Ni enriched diffusion layer was formed by the interface elements diffusion. The Cr and Ni diffusion led to the formation of ˜10 μm wide Cr and Ni layers on the carbon steel side.

Nucleation at hardness indentations in cold rolled Al

DEFF Research Database (Denmark)

Xu, C.L.; Zhang, Yubin; Wu, G.L.

2015-01-01

Nucleation of recrystallization near hardness indentations has been investigated in slightly cold rolled high purity aluminium. Samples were cold rolled to 12% and 20% reductions in thickness and indentations were done with two different loads (500 g and 2000 g). The samples were annealed at 300 °C...... for 1 h and nuclei were identified. It is found that the indentations are preferential nucleation sites. With EBSD maps around indentation tips, the orientation relationship between nuclei and matrix is analyzed. Finally, effects of rolling reduction and indentation load on local misorientations...... and stored energy distributions and thus on nucleation are discussed....

Rolling revisado: utilización del rolling para valorar y tratar la coordinación y control neuromuscular del core y extremidades en atletas

Directory of Open Access Journals (Sweden)

Barbara J. Hoogenboom

2017-05-01

Full Text Available Rolling es un patrón de movimiento raramente utilizado por los fisioterapeutas para la evaluación e intervención de pacientes con función neurológica normal. El Rolling, como destreza motriz adulta, combina el uso de las extremidades superiores, core y extremidades inferiores con el movimiento coordinado en el paso de una postura a otra. El Rolling se lleva a cabo partiendo de la posición prona a posición supina y viceversa, aunque el método utilizado varía entre adultos. Desde la perspectiva de la habilidad de completar tareas o la simetría bilateral, el Rolling puede ser beneficioso para el uso de atletas que realizan deportes de rotación parcial tales como el golf, el lanzamiento, el tenis, y los deportes con torsión como la danza, la gimnasia, y el patinaje artístico. Además, cuando es usado como técnica de intervención, los patrones del Rolling tienen la capacidad de influir en disfunciones de la parte superior del cuerpo, core y parte inferior. Aplicando los principios de la facilitación neuromuscular propioceptiva (FNP, el terapeuta puede asistir a pacientes y clientes que son incapaces de completar un patrón de Rolling. Algunos ejemplos citados en el artículo incluyen separación/elongación, compresión, y contacto manual para facilitar el propio Rolling. Los autores defienden que el uso terapéutico de los patrones de desarrollo del Rolling con las técnicas derivadas de FNP es un distintivo en la rehabilitación de pacientes con disfunciones neurológicas que pueden ser también utilizados en la rehabilitación músculo-esquelética de forma creativa y efectiva. Se han obtenido los resultados preliminares de una exploración del mecanismo por el que el Rolling puede influir en la estabilidad y existen evidencias recientes disponibles. El propósito de este comentario clínico es describir las técnicas de análisis, evaluación y tratamiento de disfunción, usando casos ejemplos que incorporan el Rolling.

Effects of alignment on the roll-over shapes of prosthetic feet.

Science.gov (United States)

Hansen, Andrew

2008-12-01

Recent work suggests that a prosthetic ankle-foot component's roll-over shape - the effective rocker it conforms to between initial contact and opposite initial contact (the 'roll-over' interval of walking) - is closely linked to its final alignment in the prosthesis (as determined by a skilled prosthetist using heuristic techniques). If true, this information may help to determine the appropriate alignment for a lower limb prosthesis before it is built, or a priori. Knowledge is needed for future models that will incorporate the roll-over shape including the relative effect of alignment on the roll-over shape's radius of curvature and arc length. The purpose of this study was to evaluate the hypotheses that: (i) Changes in prosthesis alignment alter the position and orientation of a foot's roll-over shape in prosthesis-based coordinates, and (ii) these changes occur without changing the radius of curvature or arc length of the roll-over shape. To examine the hypotheses, this study examined the effects of nine alignment settings on the roll-over shapes of two prosthetic feet. The idea that alignment changes move and rotate roll-over shapes of prosthetic feet in prosthesis coordinates is supported by this work, but the hypothesis that the radius of curvature and arc length do not change for different alignments is not strongly supported by the data. A revised approach is presented that explains some of the changes to the roll-over shape parameters due to changes in rotational alignment.

Experiment and simulation analysis of roll-bonded Q235 steel plate

International Nuclear Information System (INIS)

Zhao, G.; Huang, Q.; Zhou, C.; Zhang, Z.; Ma, L.; Wang, X.

2016-01-01

Heavy-gauge Q235 steel plate was roll bonded, and the process was simulated using MARC software. Ultrasonic testing results revealed the presence of cracks and lamination defects in an 80-mm clad steel sheet, especially at the head and tail of the steel plate. There were non-uniform ferrite + pearlite microstructures and unbound areas at a bond interface. Through scanning electron microscopy analysis, long cracks and additional inclusions in the cracks were observed at the interface. A fracture analysis revealed non-uniform inclusions that pervaded the interface. Moreover, MARC simulations demonstrated that there was little equivalent strain at the centre of the slab during the first rolling pass. The equivalent centre increased to 0.5 by the fourth rolling pass. Prior to the final pass, the equivalent strain was not consistent across the thickness direction, preventing bonding interfaces from forming consistent deformation and decreasing the residual stress. The initial rolling reduction rate should not be very small (e.g. 5%) as it is averse to the coordination of rolling deformation. Such rolling processes are averse to the rolling bond. (Author)

Correlation between Fatigue Crack Growth Behavior and Fracture Surface Roughness on Cold-Rolled Austenitic Stainless Steels in Gaseous Hydrogen

Directory of Open Access Journals (Sweden)

Tai-Cheng Chen

2018-03-01

Full Text Available Austenitic stainless steels are often considered candidate materials for use in hydrogen-containing environments because of their low hydrogen embrittlement susceptibility. In this study, the fatigue crack growth behavior of the solution-annealed and cold-rolled 301, 304L, and 310S austenitic stainless steels was characterized in 0.2 MPa gaseous hydrogen to evaluate the hydrogen-assisted fatigue crack growth and correlate the fatigue crack growth rates with the fracture feature or fracture surface roughness. Regardless of the testing conditions, higher fracture surface roughness could be obtained in a higher stress intensity factor (∆K range and for the counterpart cold-rolled specimen in hydrogen. The accelerated fatigue crack growth of 301 and 304L in hydrogen was accompanied by high fracture surface roughness and was associated with strain-induced martensitic transformation in the plastic zone ahead of the fatigue crack tip.

Recycling strategy of the end-of-life rolling stock in China

Science.gov (United States)

Guo, L.; Wang, X. W.; Lin, Y.; Shen, X. F.; Liu, Y. Q.

2018-03-01

China's high-speed railway industry is growing fast, the side effect is that plenty of rolling stock come to the end of life each year. However, there are not relevant standards nor regulations for the recycling of rolling stock in China, which causes pollution and a waste of resources. In this paper, the basic meaning and characteristics of the circular economy is reviewed and applied to the rolling stock industry. The recycling steps are elaborated in detail according to the characteristics of rolling stock. The result proves that circular economy has both the theoretical and practical meaning in the rolling stock recycling industry in China.

Metallurgical analysis of spalled work roll of hot strip mill

International Nuclear Information System (INIS)

Khan, M.M.; Khan, M.A.

1993-01-01

In this study failure analysis of four work roll of the Hot Strip Mill is carried out. The microstructure is correlated with the chemical composition of shell and roll-life. It was concluded that for the longer service of the roll, cementite, graphite and martensite should be balanced (as per working requirement of the mill). (author)

Vesilinnu teekonnalt maailma otsa / Juhani Püttsepp

Index Scriptorium Estoniae

Püttsepp, Juhani, 1964-

2011-01-01

Vassili Sarana dokumentaalfilmidest "Suur jõgi: Retk Leena lättele" ja "Delta", produtsent, helioperaator, jutustaja Riho Västrik ja monteerija Liina Triškina. Vestlusest dokumentalist Vahur Laiapeaga

Structure fields in the solidifying cast iron roll

Directory of Open Access Journals (Sweden)

W.S. Wołczyński

2010-01-01

Full Text Available Some properties of the rolls depend on the ratio of columnar structure area to equiaxed structure area created during roll solidification. The transition is fundamental phenomenon that can be apply to characterize massive cast iron rolls produced by the casting house. As the first step of simulation, a temperature field for solidifying cast iron roll was created. The convection in the liquid is not comprised since in the first approximation, the convection does not influence the studied occurrence of the (columnar to equiaxed grains transition in the roll. The obtained temperature field allows to study the dynamics of its behavior observed in the middle of the mould thickness. This midpoint of the mould thickness was treated as an operating point for the transition. A full accumulation of the heat in the mould was postulated for the transition. Thus, a plateau at the curve was observed at the midpoint. The range of the plateau existence corresponded to the incubation period , that appeared before fully equiaxed grains formation. At the second step of simulation, behavior of the thermal gradients field was studied. Three ranges within the filed were visible: EC→EC→EC→EC→(tTECtt↔RERCtt↔a/ for the formation of columnar structure (the C – zone: ( and 0>>T&0>>=−>−=REREttGttG.The columnar structure formation was significantly slowed down during incubation period. It resulted from a competition between columnar growth and equiaxed growth expected at that period of time. The 0≈=−=RERCttGttG relationship was postulated to correspond well with the critical thermal gradient, known in the Hunt’s theory. A simulation was performed for the cast iron rolls solidifying as if in industrial condition. Since the incubation divides the roll into two zones: C and E; (the first with columnar structure and the second with fully equiaxed structure some experiments dealing with solidification were made on semi-industrial scale.

Roll-to-Roll Manufacturing of Robust Superhydrophobic Coating on Metallic Engineering Materials.

Science.gov (United States)

Dong, Shuliang; Wang, Zhenlong; Wang, Yukui; Bai, Xuelin; Fu, Yong Qing; Guo, Bin; Tan, Chaoliang; Zhang, Jia; Hu, PingAn

2018-01-17

Creating a robust superhydrophobic surface on the conventional engineering materials at mass production is of great importance for a self-cleaning, anti-icing, nonwetting surface and low flow resistance in industrial applications. Herein, we report a roll-to-roll strategy to create durable and robust superhydrophobic surfaces with designed micro-/nanoscale hierarchical structures on many conventional engineering materials by combining electrical discharge machining and coating of carbon nanoparticles, followed by oil penetration and drying. The treated surface shows good superhydrophobic properties with a static water contact angle of 170 ± 2° and slide angle of 3 ± 1°. The treated surface also exhibits good resilience and maintains the performance after being tested in various harsh conditions, including water flushing for several days, sand abrasion, scratching with sandpapers, and corrosive solution. Significantly, the superhydrophobic surfaces also show a high efficiency of self-cleaning properties even after oil contamination during applications.

Semi-solid twin-roll casting process of magnesium alloy sheets

International Nuclear Information System (INIS)

Watari, H.; Davey, K.; Rasgado, M.T. Alonso; Haga, T.; Koga, N.

2004-01-01

An experimental approach has been performed to ascertain the effectiveness of semi-solid strip casting using a horizontal twin roll caster. The demand for light-weight products with high strength has grown recently due to the rapid development of automobile and aircraft technology. One key to such development has been utilization of magnesium alloys, which can potentially reduce the total product weight. However, the problems of utilizing magnesium alloys are still mainly related to high manufacturing cost. One of the solutions to this problem is to develop magnesium casting-rolling technology in order to produce magnesium sheet products at competitive cost for commercial applications. In this experiment, magnesium alloy AZ31B was used to ascertain the effectiveness of semi-solid roll strip casting for producing magnesium alloy sheets. The temperature of the molten magnesium, and the roll speeds of the upper and lower rolls, (which could be changed independently), were varied to find an appropriate manufacturing condition. Rolling and heat treatment conditions were changed to examine which condition would be appropriate for producing wrought magnesium alloys with good formability. Microscopic observation of the crystals of the manufactured wrought magnesium alloys was performed. It has been found that a limiting drawing ratio of 2.7 was possible in a warm deep drawing test of the cast magnesium alloy sheets after being hot rolled

Effects of gamma and electron beam irradiation on the microbial quality of steamed tofu rolls

International Nuclear Information System (INIS)

Jia, Qian; Gao, Meixu; Li, Shurong; Wang, Zhidong

2013-01-01

The effectiveness of two kinds of radiation processing, gamma and electron beam (ebeam) irradiation, for the inactivation of Staphylococcus aureus, Salmonella enteritidis and Listeria innocua which were inoculated in pre-sterilised steamed tofu rolls was studied. The corresponding effects of both irradiation types on total bacterial counts (TBCs) in commercial steamed tofu rolls available in the market were also examined. The microbiological results demonstrated that gamma irradiation yielded D 10 values of 0.20, 0.24 and 0.22 kGy for S. aureus, S. enteritidis and L. innocua, respectively. The respective D 10 values for ebeam irradiation were 0.31, 0.35 and 0.27 kGy. Gamma and ebeam irradiation yielded D 10 values of 0.48 and 0.43 kGy for total bacterial counts in commercial steamed tofu rolls, respectively. The results suggest that ebeam irradiation has similar effect on decreasing TBCs in steamed tofu rolls, and gamma irradiation is slightly more effective than ebeam irradiation in reducing the populations of pathogenic bacteria. The observed differences in D 10 -values between them might be due to the significant differences in dose rate applied, and radiation processing of soybean products to improve their microbial quality could be available for other sources of protein. - Highlights: ► Our research material is steamed tofu rolls, a kind of soybean products. ► We compared the effects of gamma ray and electron beam irradiation. ► Total bacterial and three strains of pathogens are studied in our research. ► We reported electron beam has similar decontamination effect as gamma ray. ► Radiation processing of soybean products to improve their microbial quality could be available for other sources of protein.

Texture evolution of experimental silicon steel grades. Part I: Hot rolling

Energy Technology Data Exchange (ETDEWEB)

Sandoval Robles, J.A., E-mail: jsandoval.uanl@yahoo.com [Universidad Autónoma de Nuevo León, Facultad de Ingeniería Mecánica y Eléctrica, Ave. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66450 (Mexico); Salas Zamarripa, A.; Guerrero Mata, M.P. [Universidad Autónoma de Nuevo León, Facultad de Ingeniería Mecánica y Eléctrica, Ave. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66450 (Mexico); Cabrera, J. [Universitat Politècnica de Catalunya, Departament de Ciència dels Materials I Enginyeria Metal-lúrgica, Av. Diagonal 647, Barcelona 08028 (Spain)

2017-05-01

The metallurgical understanding of the deformation processes during the fabrication of non-oriented electrical steels plays a key role in improving their final properties. Texture control and optimization is critical in these steels for the enhancement of their magnetic properties. The aim of the present work is to study the texture evolution of six non-oriented experimental silicon steel grades during hot rolling. These steels were low carbon steel with a silicon content from 0.5 to 3.0 wt%. The first rolling schedule was performed in the austenitic (γ-Fe) region for the steel with a 0.5 wt% of silicon content, while the 1.0 wt% silicon steel was rolled in the two-phase (α+γ) region. Steels with higher silicon content were rolled in the ferritic (α-Fe) region. The second rolling schedule was performed in the α-Fe region. Samples of each stage were analyzed by means of Electron Backscatter Diffraction (EBSD). Findings showed that the texture was random and heterogeneous in all samples after 60% of rolling reduction, which is due to the low deformation applied during rolling. After the second rolling program, localized deformation and substructured grains near to surface were observed in all samples. The Goss {110}<001>texture-component was found in the 0.5 and 1.0 wt.-%silicon steels. This is due to the thermomechanical conditions and the corresponding hot band microstructure obtained after the first program. Moreover, the α<110>//RD and the γ <111>//ND fiber components of the texture presented a considerable increment as the silicon content increases. Future research to be published soon will be related to the texture evolution during the cold-work rolling process. - Highlights: • We analyze six silicon steel experimental grades alloys trough the rolling process. • Material was subjected to a hot deformation process in the α-γ region. • No recrystalization was observed during-after the rolling schedules. • Rise of the magnetic texture components

Pank väärtustab kohalikku metsatööstust / Allan Ventsel

Index Scriptorium Estoniae

Ventsel, Allan

2003-01-01

Autor tõestab Hansapanga ja metsatööstuse näitel, et ettevõtte ja panga vahelistes suhetes on oluline roll tööstusharu üldisel olukorral. Autori hinnangul on metsatööstusettevõtted leidnud Hansapanga poolt finantseerimist kuna tööstusharu peetakse perspektiivseks ning seal tegelevaid ettevõtteid paindlikeks ning elujõulisteks. Diagrammid: puidu-, tselluloosi- ja paberitoodete tootmine aastail 1992-2000; metsal baseeruva tööstuse osakaal Eesti töötleva tööstuse lisandväärtusest protsentides. Lisa: Eesti metsatööstusel on suur potentsiaal

Triiodothyronine increases mRNA and protein leptin levels in short time in 3T3-L1 adipocytes by PI3K pathway activation.

Directory of Open Access Journals (Sweden)

Miriane de Oliveira

Full Text Available The present study aimed to examine the effects of thyroid hormone (TH, more precisely triiodothyronine (T3, on the modulation of leptin mRNA expression and the involvement of the phosphatidyl inositol 3 kinase (PI3K signaling pathway in adipocytes, 3T3-L1, cell culture. We examined the involvement of this pathway in mediating TH effects by treating 3T3-L1 adipocytes with physiological (P=10nM or supraphysiological (SI=100 nM T3 dose during one hour (short time, in the absence or the presence of PI3K inhibitor (LY294002. The absence of any treatment was considered the control group (C. RT-qPCR was used for mRNA expression analyzes. For data analyzes ANOVA complemented with Tukey's test was used at 5% significance. T3 increased leptin mRNA expression in P (2.26 ± 0.36, p 0.001. These results demonstrate that the activation of the PI3K signaling pathway has a role in TH-mediated direct and indirect leptin gene expression in 3T3-L1 adipocytes.

Synergy between plasma-assisted ALD and roll-to-roll atmospheric pressure PE-CVD processing of moisture barrier films on polymers

NARCIS (Netherlands)

Starostin, S.A.; Keuning, W.; Schalken, J.R.G.; Creatore, M.; Kessels, W.M.M.; Bouwstra, J.B.; Sanden, van de M.C.M.; Vries, de H.W.

2016-01-01

The synergy between fast (1600 nm · min−1), roll-to-roll plasma-enhanced chemical vapor deposited (PE-CVD) SiO2 layers and plasma-assisted atomic layer deposited (PA-ALD) ultra-thin Al2O3 films has been investigated in terms of moisture permeation barrier properties. The effective and intrinsic

Synergy Between Plasma-Assisted ALD and Roll-to-Roll Atmospheric Pressure PE-CVD Processing of Moisture Barrier Films on Polymers

NARCIS (Netherlands)

Starostin, S. A.; Keuning, W.; Schalken, J.; Creatore, M.; Kessels, W. M. M.; Bouwstra, J. B.; van de Sanden, M. C. M.; de Vries, H. W.

2016-01-01

The synergy between fast (1600 nm · min−1), roll-to-roll plasma-enhanced chemical vapor deposited (PE-CVD) SiO2 layers and plasma-assisted atomic layer deposited (PA-ALD) ultra-thin Al2O3 films has been investigated in terms of moisture permeation barrier properties. The effective and intrinsic

The record of iceberg roll generated waves from sediments and seismics

Science.gov (United States)

Rosser, N. J.; Szczucinski, W.; Strzelecki, M.; Long, A. J.; Norman, E. C.; Dunning, S.; Drewniak, M.

2013-12-01

Iceberg-roll tsunamis in coastal settings have been observed to generate significant local waves, that hold potential to be recorded in coastal depositional records. Capturing the past magnitude and frequency of such events remains challenging, hindered by a lack of a good understanding of the nature, recurrence and scale of iceberg rolls, and more specifically those rolls that generate waves. Here we consider the sedimentary evidence for iceberg rolls in West Central Greenland, based upon survey of depositional environments in a range of open and confined coastal environments. We examine both an open 80 km fjord setting, and a series of confined ice-marginal beaches. We combine a detailed interpretation of sediment deposits from shore-normal transects with wider-scale high-resolution terrestrial laser scanning of sediments. Our sites - Vaigat, which separates Disko Island from the Nussuaq Peninsular, and the northern shore of Icefjord - both have a recent history of tsunamis, triggered variously by large rock avalanches, landslides and iceberg rolls. Icebergs in Vaigat and Icefjord are observed to undergo frequent failure and roll, generating - where circumstances permit - nearshore waves of meter-scale. To obtain a more detailed understanding of the likely recurrence of such iceberg roll waves and to consider their influence upon the preserved sedimentary record, we undertook an intensive 2-month monitoring campaign during sea-ice free conditions in summer 2013 to determine the patterns in the location, magnitude, frequency and timing of iceberg roll waves. Innovatively, using microseismic monitoring combined with time-lapse photography and weather monitoring, we derive a first-order model of the occurrence of iceberg roll waves. We then use this to inform our interpretation of deposits in these two environments, and consider the presence and absence of records of iceberg roll deposits in such settings. The study was funded by Polish National Science Centre grant

Prediction of Ship Resonant Rolling - Related Dangerous Zones with Regard to the Equivalent Metacentric Height Governing Natural Frequency of Roll

Directory of Open Access Journals (Sweden)

Przemyslaw Krata

2017-12-01

Full Text Available Potentially dangerous zones corresponding to dynamical stability phenomena, possibly encountered by ships sailing in rough sea, are estimated nowadays with the use of the method recommended by IMO in the guidance coded MSC.1/Circ.1228. In this IMO method the parameter governing the natural period of roll is the initial metacentric height. Some earlier studies revealed that the initial metacentric height which is commonly in use on-board ships for the purpose of performing the MSC.1/Circ.1228-recommended calculations, may significantly vary from the so called equivalent metacentric height obtained for large amplitudes of ship’s roll. In the light of such ascertainment, the paper deals with resultant resonance roll zones locations with regard to the equivalent metacentric height concept remaining appropriate for large amplitudes of roll. The noteworthy transfer of the resonance zones location is disclosed which reflects the distinct configurations of potentially dangerous ship’s course and speed configurations than could be predicted on the basis the initial metacentric height.

Rolled-up transmission line structure for a radiofrequency integrated circuit (RFIC)

Science.gov (United States)

Li, Xiuling; Huang, Wen

2015-04-28

A rolled-up transmission line structure for a radiofrequency integrated circuit (RFIC) comprises a multilayer sheet in a rolled configuration comprising multiple turns about a longitudinal axis, where the multilayer sheet comprises a conductive pattern layer on a strain-relieved layer. The conductive pattern layer comprises a first conductive film and a second conductive film separated from the first conductive film in a rolling direction. In the rolled configuration, the first conductive film surrounds the longitudinal axis, and the second conductive film surrounds the first conductive film. The first conductive film serves as a signal line and the second conductive film serves as a conductive shield for the rolled-up transmission line structure.

Research and industrialization of near-net rolling technology used in shaft parts

Science.gov (United States)

Hu, Zhenghuan; Wang, Baoyu; Zheng, Zhenhua

2018-03-01

Shaft part rolling is an efficient and green nearnet shaping technology offering many advantages, including high production efficiency, high material utilization rate, high product quality, and excellent production environment. In this paper, the features of shaft part rolling are introduced along with the working principles of two main shaft part rolling technologies, namely, cross wedge rolling (CWR) and skew rolling (SR). In relation to this technology, some R&D achievements gained by the University of Science and Technology Beijing are summarized. Finally, the latest developments in shaft part rolling are presented, including SR steel balls, precise forming of camshaft blank by CWR, SR phosphorous copper balls at room temperature, and CWR hollow axle sleeve. Although the shaft part rolling technology has been widely used in China, it only accounts for about 15% of applicable parts at present. Nevertheless, this technology has broad application prospects.

Effects of rolling on single-phase water forced convective heat transfer characteristics

International Nuclear Information System (INIS)

Guo Yanming; Gao Puzhen; Huang Zhen

2010-01-01

A series of single-phase forced circulation tests in a vertical tube with rolling motion were performed in order to investigate effects of rolling motion on thermal-hydraulic characteristics. The amplitudes of the rolling motion in the tests were 10 degree, 15 degree and 20 degree. The rolling periods were 7.5 s, 10 s, 15 s and 20 s. The Reynolds number was from 6000 to 15000. Heat transfer in the test tube is bated by the rolling motion. As the test-bed rolling more acutely, the heat transfer coefficient of the test tube becomes smaller when the mass flow rate in the test tube is a constant. The heat transfer coefficient calculated by the formula which is for stable state doesn't fit very well with that from experiments. At last a formula for calculating heat transfer in rolling motion was introduced. (authors)

RNA polymerase of the killer virus of yeast

International Nuclear Information System (INIS)

Georgopoulos, D.E.; Leibowitz, M.J.

1984-01-01

The L/sub A/ and M double-stranded (ds) RNA segments of the cytoplasmically inherited killer virus of Saccharomyces cerevisiae are encapsidated in virions that contain a DNA-independent transcriptase activity. This enzyme catalyzes the synthesis of full-length (+) stranded copies of the genomic dsRNA segments, denoted l/sub A/ and m. The L/sub A/ dsRNA segment appears to encode the major capsid protein in which both dsRNA molecules are encapsidated, while M dsRNA encodes products responsible for the two killer phenotypes of toxin production and resistance to toxin. Proteins extracted from transcriptionally active virions fail to cross-react with antibody to yeast DNA-dependent RNA polymerases, suggesting that none of the subunits of the host cell polymerases are active in viral transcription. Sequence analysis of the in vitro transcripts reveals neither to be 3'-terminally polyadenylated, although m contains an apparent internal polyA-like tract. In the presence of any three ribonucleoside triphosphates (0.5 mM), the fourth ribonucleoside triphosphate shows an optimal rate of incorporation into transcript at a concentration of 20 μM. However, in a 3-hour reaction, the yield of a product RNA increases with the concentration of the limiting ribonucleotide up to 0.5 mM. Gel electrophoresis of the reaction products reveals that increasing the substrate concentration accelerates the appearance of radioactivity in full-length l/sub A/ and m transcripts

Metal-assisted exfoliation (MAE): green, roll-to-roll compatible method for transferring graphene to flexible substrates

International Nuclear Information System (INIS)

Zaretski, Aliaksandr V; Moetazedi, Herad; Kong, Casey; Sawyer, Eric J; Savagatrup, Suchol; Valle, Eduardo; O’Connor, Timothy F; Printz, Adam D; Lipomi, Darren J

2015-01-01

Graphene is expected to play a significant role in future technologies that span a range from consumer electronics, to devices for the conversion and storage of energy, to conformable biomedical devices for healthcare. To realize these applications, however, a low-cost method of synthesizing large areas of high-quality graphene is required. Currently, the only method to generate large-area single-layer graphene that is compatible with roll-to-roll manufacturing destroys approximately 300 kg of copper foil (thickness = 25 μm) for every 1 g of graphene produced. This paper describes a new environmentally benign and scalable process of transferring graphene to flexible substrates. The process is based on the preferential adhesion of certain thin metallic films to graphene; separation of the graphene from the catalytic copper foil is followed by lamination to a flexible target substrate in a process that is compatible with roll-to-roll manufacturing. The copper substrate is indefinitely reusable and the method is substantially greener than the current process that uses relatively large amounts of corrosive etchants to remove the copper. The sheet resistance of the graphene produced by this new process is unoptimized but should be comparable in principle to that produced by the standard method, given the defects observable by Raman spectroscopy and the presence of process-induced cracks. With further improvements, this green, inexpensive synthesis of single-layer graphene could enable applications in flexible, stretchable, and disposable electronics, low-profile and lightweight barrier materials, and in large-area displays and photovoltaic modules. (paper)

Metal-assisted exfoliation (MAE): green, roll-to-roll compatible method for transferring graphene to flexible substrates

Science.gov (United States)

Zaretski, Aliaksandr V.; Moetazedi, Herad; Kong, Casey; Sawyer, Eric J.; Savagatrup, Suchol; Valle, Eduardo; O'Connor, Timothy F.; Printz, Adam D.; Lipomi, Darren J.

2015-01-01

Graphene is expected to play a significant role in future technologies that span a range from consumer electronics, to devices for the conversion and storage of energy, to conformable biomedical devices for healthcare. To realize these applications, however, a low-cost method of synthesizing large areas of high-quality graphene is required. Currently, the only method to generate large-area single-layer graphene that is compatible with roll-to-roll manufacturing destroys approximately 300 kg of copper foil (thickness = 25 μm) for every 1 g of graphene produced. This paper describes a new environmentally benign and scalable process of transferring graphene to flexible substrates. The process is based on the preferential adhesion of certain thin metallic films to graphene; separation of the graphene from the catalytic copper foil is followed by lamination to a flexible target substrate in a process that is compatible with roll-to-roll manufacturing. The copper substrate is indefinitely reusable and the method is substantially greener than the current process that uses relatively large amounts of corrosive etchants to remove the copper. The sheet resistance of the graphene produced by this new process is unoptimized but should be comparable in principle to that produced by the standard method, given the defects observable by Raman spectroscopy and the presence of process-induced cracks. With further improvements, this green, inexpensive synthesis of single-layer graphene could enable applications in flexible, stretchable, and disposable electronics, low-profile and lightweight barrier materials, and in large-area displays and photovoltaic modules.

Kriis sunnib mobiilitootjaid ühinema või loobuma / Tõnis Arnover

Index Scriptorium Estoniae

Arnover, Tõnis, 1952-

2001-01-01

Kuna suured investeeringud kolmanda põlvkonna 3G mobiiltelefonide arengusse on langenud kokku mobiiltelefonide müügi kahanemisega, peavad mitu mobiilitootjat ühinemise või tegevuse lõpetamise plaani

Influence of rolling and annealing conditions on texture and mechanical properties of zirconium (1960); Influence des conditions de laminage et de recuit sur la texture et les proprietes mecaniques du zirconium (1960)

Energy Technology Data Exchange (ETDEWEB)

Orssaud, J [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1958-06-15

Rolling and annealing textures of KROLL zirconium samples at several rolling rates were studied by pole figures with an automatic recorder versus the position in the sheet thickness. Tensile tests, hardness measurements and micrographic examinations allowed to study the evolution of the recrystallization and the variation of the mechanical properties after rolling and/or annealing. Annealing textures slightly varies with the annealing temperature. Annealing at 500 deg. C gives several peculiarities. This temperature seems characteristic in the study of zirconium. (author) [French] Les textures de laminage et de recuit d'echantillons de zirconium KROLL lamine a divers taux ont ete etudiees en tracant des diagrammes de figures de poles en divers points de l'epaisseur des toles, a l'aide d'un appareil a enregistrement automatique. Des essais de traction et des mesures de duretes ainsi que des micrographies ont permis de suivre l'evolution de la recristallisation et les variations des caracteristiques mecaniques au divers stades du laminage et du recuit. La texture de recuit parait varier avec la temperature utilisee. De nombreuses particularites qui apparaissent apres recuit vers 500 deg. C montrent l'importance de cette zone de temperature dans l'etude du zirconium. (auteur)

In situ monitoring of structure formation in the active layer of polymer solar cells during roll-to-roll coating

DEFF Research Database (Denmark)

Rossander, Lea Hildebrandt; Zawacka, Natalia Klaudia; Dam, Henrik Friis

2014-01-01

The active layer crystallization during roll-to-roll coating of organic solar cells is studied in situ. We developed an X-ray setup where the coater unit is an integrated part of the small angle X-ray scattering instrument, making it possible to control the coating process while recording...

Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery

DEFF Research Database (Denmark)

Chen, Menglin; Gao, Shan; Dong, Mingdong

2012-01-01

Composite nanofibers of biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) encapsulating chitosan/siRNA nanoparticles (NPs) were prepared by electrospinning. Acidic/alkaline hydrolysis and a bulk/surface degradation mechanism were investigated in order to achieve an optimized release profile...... for prolonged and efficient gene silencing. Thermo-controlled AFM in situ imaging not only revealed the integrity of the encapsulated chitosan/siRNA polyplex but also shed light on the decreasing Tg of PLGA on the fiber surfaces during release. A triphasic release profile based on bulk erosion was obtained at p......RNA transfection, where the encapsulated chitosan/siRNA NPs exhibited up to 50% EGFP gene silencing activity after 48 h post-transfection on H1299 cells....

Closure behavior of spherical void in slab during hot rolling process

Science.gov (United States)

Cheng, Rong; Zhang, Jiongming; Wang, Bo

2018-04-01

The mechanical properties of steels are heavily deteriorated by voids. The influence of voids on the product quality should be eliminated through rolling processes. The study on the void closure during hot rolling processes is necessary. In present work, the closure behavior of voids at the center of a slab at 800 °C during hot rolling processes has been simulated with a 3D finite element model. The shape of the void and the plastic strain distribution of the slab are obtained by this model. The void decreases along the slab thickness direction and spreads along the rolling direction but hardly changes along the strip width direction. The relationship between closure behavior of voids and the plastic strain at the center of the slab is analyzed. The effects of rolling reduction, slab thickness and roller diameter on the closure behavior of voids are discussed. The larger reduction, thinner slab and larger roller diameter all improve the closure of voids during hot rolling processes. Experimental results of the closure behavior of a void in the slab during hot rolling process mostly agree with the simulation results..

On-Particle Rolling Circle Amplification-Based Core-Satellite Magnetic Superstructures for MicroRNA Detection

DEFF Research Database (Denmark)

Tian, Bo; Qiu, Zhen; Ma, Jing

2018-01-01

-specific nuclease in the presence of target microRNA, resulting in a release of MNPs that can be quantified in an optomagnetic sensor. The proposed biosensor has a simple mix-separate-measure strategy. For let-7b detection, the proposed biosensor offers a wide linear detection range of approximately 5 orders...

Kui riskantne on India? / Mehul Srivastava, Nandini Lakshman

Index Scriptorium Estoniae

Srivastava, Mehul

2008-01-01

Seni suure majanduskasvuga välisinvestoreid ja välisettevõtjaid ahvatlenud India on pärast 26. novembril 2008 aset leidnud tulistamisi Mumbai lõunaosas sattunud vastamisi olukorraga, kus on paljastunud lõhe riigi positiivse maine ja reaalsuse vahel. Aruteludest riigi tuleviku üle

Assembly of proteins and 5 S rRNA to transcripts of the major structural domains of 23 S rRNA

DEFF Research Database (Denmark)

Ostergaard, P; Phan, H; Johansen, L B

1998-01-01

The six major structural domains of 23 S rRNA from Escherichia coli, and all combinations thereof, were synthesized as separate T7 transcripts and reconstituted with total 50 S subunit proteins. Analysis by one and two-dimensional gel electrophoresis demonstrated the presence of at least one prim...... approach was used to map the putative binding regions on domain V of protein L9 and the 5 S RNA-L5-L18 complex....

Nonlinear Container Ship Model for the Study of Parametric Roll Resonance

DEFF Research Database (Denmark)

Holden, Christian; Galeazzi, Roberto; Rodríguez, Claudio

2007-01-01

Parametric roll is a critical phenomenon for ships, whose onset may cause roll oscillations up to 40, leading to very dangerous situations and possibly capsizing. Container ships have been shown to be particularly prone to parametric roll resonance when they are sailing in moderate to heavy head ...

Experimental and theoretical study on natural circulation capacity under rolling motion condition

International Nuclear Information System (INIS)

Tan Sichao; Gao Puzhen

2007-01-01

Effect of rolling motion on natural circulation capacity was studied experimentally and theoretically. Experiments were conducted under the conditions of rolling and unrolling motions. The experimental results show that natural circulation capacity decreases under rolling motion condition. A mathematic model was developed to calculate the natural circulation capacity under rolling motion condition, considering the characteristics of natural circulation, the model was modified. The calculated results agree with experimental data well. Effect of rolling motion on natural circulation was analyzed through calculation and the following conclusions were obtained: (1) The increase of flow resistance coefficient is the main reason that the natural circulation capacity decreases under rolling motion condition; (2) Non-uniform distribution of fluid mass in the pipe has also influence on natural circulation capacity. (author)

Nonlinear Container Ship Model for the Study of Parametric Roll Resonance

Directory of Open Access Journals (Sweden)

Christian Holden

2007-10-01

Full Text Available Parametric roll is a critical phenomenon for ships, whose onset may cause roll oscillations up to +-40 degrees, leading to very dangerous situations and possibly capsizing. Container ships have been shown to be particularly prone to parametric roll resonance when they are sailing in moderate to heavy head seas. A Matlab/Simulink parametric roll benchmark model for a large container ship has been implemented and validated against a wide set of experimental data. The model is a part of a Matlab/Simulink Toolbox (MSS, 2007. The benchmark implements a 3rd-order nonlinear model where the dynamics of roll is strongly coupled with the heave and pitch dynamics. The implemented model has shown good accuracy in predicting the container ship motions, both in the vertical plane and in the transversal one. Parametric roll has been reproduced for all the data sets in which it happened, and the model provides realistic results which are in good agreement with the model tank experiments.

Effect of rolling motion on the expansion and contraction loss coefficients

International Nuclear Information System (INIS)

Yan, B.H.; Gu, H.Y.

2013-01-01

Highlights: ► The expansion and contraction loss coefficients in rolling motion are analyzed. ► Effects of rolling motion on the expansion and contraction loss coefficients are different. ► The spanwise and transverse additional forces contribute slightly to the local loss. ► The oscillations of loss coefficients increase as the strengthening of rolling motion. - Abstract: The sudden expansion and sudden contraction loss coefficients in rolling motion are investigated with CFD code FLUENT. The calculation results are validated with experimental and theoretical results in steady state. The effects of rolling motion on the expansion and contraction loss coefficients are different. The effects of spanwise and transverse additional forces on the expansion and contraction loss coefficients are weak. The effect of velocity oscillation on the contraction loss coefficient is more significant than that on the expansion loss coefficient. The oscillation of local loss coefficient also becomes more and more irregular as the strengthening of rolling motion

Estimation of Parametric Roll in a Stochastic Seaway

DEFF Research Database (Denmark)

Jensen, Jørgen Juncher; Pedersen, Preben Terndrup; Vidic-Perunovic, Jelena

2008-01-01

-degree of freedom (roll and heave) time domain model [10]. In the present paper the effect of the increased added resistance when the bow heaves and pitches down in a wave crest is introduced. Due to the resulting forward speed variation the roll resonance condition will be changed. The influence of ship speed...

Direct and precise measurement of displacement and velocity of flexible web in roll-to-roll manufacturing systems

International Nuclear Information System (INIS)

Kang, Dongwoo; Lee, Eonseok; Choi, Young-Man; Lee, Taik-Min; Kim, Duk Young; Kim, Dongmin

2013-01-01

Interest in the production of printed electronics using a roll-to-roll system has gradually increased due to its low mass-production costs and compatibility with flexible substrate. To improve the accuracy of roll-to-roll manufacturing systems, the movement of the web needs to be measured precisely in advance. In this paper, a novel measurement method is developed to measure the displacement and velocity of the web precisely and directly. The proposed algorithm is based on the traditional single field encoder principle, and the scale grating has been replaced with a printed grating on the web. Because a printed grating cannot be as accurate as a scale grating in a traditional encoder, there will inevitably be variations in pitch and line-width, and the motion of the web should be measured even though there are variations in pitch and line-width in the printed grating patterns. For this reason, the developed algorithm includes a precise method of estimating the variations in pitch. In addtion, a method of correcting the Lissajous curve is presented for precision phase interpolation to improve measurement accuracy by correcting Lissajous circle to unit circle. The performance of the developed method is evaluated by simulation and experiment. In the experiment, the displacement error was less than 2.5 μm and the velocity error of 1σ was about 0.25%, while the grating scale moved 30 mm

Direct and precise measurement of displacement and velocity of flexible web in roll-to-roll manufacturing systems

Energy Technology Data Exchange (ETDEWEB)

Kang, Dongwoo; Lee, Eonseok; Choi, Young-Man; Lee, Taik-Min [Advanced Manufacturing Systems Research Division, Korea Institute of Machinery and Materials, 156 Gajeongbuk-Ro, Yuseong-Gu, Daejeon 305-343 (Korea, Republic of); Kim, Duk Young [Nano-Opto-Mechatronics Lab., Dept. of Mechanical Eng., KAIST, 335 Gwahangno, Yuseong-Gu, Daejeon 305-701 (Korea, Republic of); Kim, Dongmin [Korea Research Institute of Standards and Science, 267 Gajeong-Ro, Yuseong-Gu, Daejeon 305-340 (Korea, Republic of)

2013-12-15

Interest in the production of printed electronics using a roll-to-roll system has gradually increased due to its low mass-production costs and compatibility with flexible substrate. To improve the accuracy of roll-to-roll manufacturing systems, the movement of the web needs to be measured precisely in advance. In this paper, a novel measurement method is developed to measure the displacement and velocity of the web precisely and directly. The proposed algorithm is based on the traditional single field encoder principle, and the scale grating has been replaced with a printed grating on the web. Because a printed grating cannot be as accurate as a scale grating in a traditional encoder, there will inevitably be variations in pitch and line-width, and the motion of the web should be measured even though there are variations in pitch and line-width in the printed grating patterns. For this reason, the developed algorithm includes a precise method of estimating the variations in pitch. In addtion, a method of correcting the Lissajous curve is presented for precision phase interpolation to improve measurement accuracy by correcting Lissajous circle to unit circle. The performance of the developed method is evaluated by simulation and experiment. In the experiment, the displacement error was less than 2.5 μm and the velocity error of 1σ was about 0.25%, while the grating scale moved 30 mm.

Theoretical Analysis of Unit Friction Force Working on the Metal Contact Surface with the Roll Change during Feedstock with Non-Uniform Temperature Distribution Rolling Process

Directory of Open Access Journals (Sweden)

Sygut P.

2016-06-01

Full Text Available The paper presents the results of theoretical studies influence of non-uniform temperature distribution along the feedstock length to the unit friction force working on the metal contact surface with the roll change during the round bars 70 mm in diameter continuous rolling process. This value is one of the major factors affecting the grooves wear during the rolling process. The studies were carried out based on the actual engineering data for 160 × 160 mm square cross-section feedstock of steel S355J0. Numerical modelling of the rolling process was performed using Forge2008®, a finite-element based computer program.

Interlayer adhesion in roll-to-roll processed flexible inverted polymer solar cells

KAUST Repository

Dupont, Stephanie R.

2012-02-01

The interlayer adhesion of roll-to-roll processed flexible inverted P3HT:PCBM bulk heterojunction (BHJ) polymer solar cells is reported. Poor adhesion between adjacent layers may result in loss of device performance from delamination driven by the thermomechanical stresses in the device. We demonstrate how a thin-film adhesion technique can be applied to flexible organic solar cells to obtain quantitative adhesion values. For the P3HT:PCBM-based BHJ polymer solar cells, the interface of the BHJ with the conductive polymer layer PEDOT:PSS was found to be the weakest. The adhesion fracture energy varied from 1.6 J/m2 to 0.1 J/m2 depending on the composition of the P3HT:PCBM layer. Post-deposition annealing time and temperature were shown to increase the adhesion at this interface. Additionally the PEDOT:PSS cells are compared with V2O5 cells whereby adhesive failure marked by high fracture energies was observed. © 2011 Elsevier B.V.

Occurrence, Biology and Harmfulness of Byctiscus betulae (L. (Coleoptera, Rhynchitidae

Directory of Open Access Journals (Sweden)

Jaroslav Urban

2015-01-01

Full Text Available In 2012 and 2013, was studied the occurrence, biology and harmfulness of Byctiscus betulae (L. (Rhynchitidae on forest woody plants in the Brno region. Leaf rolls were found on 13 species (and 10 genera of woody plants. Most frequently, they occurred on Fagus sylvatica, Tilia spp. (namely on T. cordata, Salix caprea and Populus tremula. The beetles hibernate in ground pupal chambers and appear on woody plants in the last decade of April. After hibernation, females live on average seven (males five weeks, and damage on average 40 (males 25 cm2 of leaves. The females create leaf rolls on annual shoots they have gnawed, exceptionally (in 2% on leaves with damaged petioles. In the rolls on F. sylvatica, they roll on average 5.6 leaves whose total average area is 49.5 cm2 and lay on average 5.1 eggs. In the rolls on Tilia spp., they roll on average 3.3 leaves whose total area is 63.2 cm2 and lay on average 4.9 eggs. In the rolls on S. caprea, they roll on average 3.3 leaves whose total area is 38.3 cm2 and lay on average 3.5 eggs. The maximum number of laid eggs (on average 8.0 was observed in the leaf rolls on Vitis vinifera. The average number of eggs in the leaf rolls was decreasing from May to July. The number of eggs was increasing with the increasing total leaf area rolled. The females lay on average 50 eggs. On P. tremula, S. caprea and Pyrus pyraster they create about 15 leaf rolls, on F. sylvatica and Tilia spp. about 10 leaf rolls and on V. vinifera ca. 8 leaf rolls. Embryogenesis lasts from 8 to 9 days. Larvae develop over 3 instars and damage ca. 300 mm2 of leaves. Coming of age within 4−7 weeks, they leave the leaf rolls from mid-June to the beginning of September.

THE THEORETICAL FOUNDATIONS OF VIBRATION DAMPERS BY ROLLING FRICTION

Directory of Open Access Journals (Sweden)

L. M. Bondarenko

2015-06-01

Full Text Available Purpose. There are some unresolved issues in vibration damping – the lack of engineering calculations for the vibration dampers by rolling friction; the absence of evidence of their application appropriateness. Considering this fact, the authors suggest to prove that the dampers based on rolling friction, are similar in rate of oscillation damping by hydraulic shock absorbers. At the same time, they are easier for the hydraulic design, and easily amenable to manual adjustment, both in automatic and manual mode. Methodology. Fixed techniques of practice in order to determine amplitudes of the oscillations of a shock absorber led to a predetermined result and will apply this theory in the calculation of other vibration dampers. Findings. Analysis of the formulas and graphs leads to the following conclusions and recommendations: 1 the nature of the oscillation damping at vibration dampers by rolling friction is close to their decay in the viscous resistance; 2 when conducting the necessary experiments the shock absorber rolling can be recommended as alternatives to hydraulic ones. The research results of this task will help implement the new trend in reduction of dynamic loads in vehicles. Originality. With the help of theoretical curves to determine the coefficients of rolling friction the dependences for determining the amplitudes of the oscillations in the vertical movement of cargo were obtained. At the same time, the previously proposed analytical dependence for determining the coefficient of rolling friction contains only conventional mechanical constants of the contacting bodies and there geometrical dimensions. Practical value. Due to the existing well-known disadvantages of hydraulic shock absorbers it would be logical to apply shock absorbers that are technologically convenient in manufacturing and easy to adjust the damping rate. The proposed theory can be used in the design of shock absorbers rolling as an alternative to the hydraulic

Venemaa saatis kaks kaugpommitajat Venezuelasse, NATO lennukid sabas / Kaivo Kopli

Index Scriptorium Estoniae

Kopli, Kaivo

2008-01-01

Kaks Venemaa pommilennukit lendasid Venezuelasse, novembris läheb Venezuelasse õppustele Vene sõjalaevade eskaader. Vaatlejate hinnangul on tegemist demonstratiivse vastusega USA sõjalaevade saabumisele Mustale merele. Lisa: Ristleja Peeter Suur

Inkjet printing as a roll-to-roll compatible technology for the production of large area electronic devices on a pre-industrial scale

NARCIS (Netherlands)

Teunissen, P.; Rubingh, E.; Lammeren, T. van; Abbel, R.J.; Groen, P.

2014-01-01

Inkjet printing is a promising approach towards the solution processing of electronic devices on an industrial scale. Of particular interest is the production of high-end applications such as large area OLEDs on flexible substrates. Roll-to-roll (R2R) processing technologies involving inkjet

Prediction of Critical Heat Flux under Rolling Motion

Energy Technology Data Exchange (ETDEWEB)

Hwang, Jinseok; Lee, Yeongun; Park, Gooncherl [Seoul National Univ., Seoul (Korea, Republic of)

2013-05-15

The aim to this paper may be summarized as follows: identify the flow regime compare with existing void-quality relationship and void fraction at OAF derived from the vapor superficial velocity obtained by the churn-to annular flow criterion, develop and evaluate the correlation for accurate prediction of CHF ratio under rolling motion. Experimentally measured CHF results from the previous study were not well-predicted by existing CHF correlations developed for wide range of pressure under rolling motion in vertical tube. Specifically, existing correlations do not account for the dynamic motion parameter, such as tangential and centrifugal force. This study reviewed some existing correlation and experimental studies related to reduction and enhancement of CHF and heat transfer and flow behavior under heaving and rolling motion, and developed a CHF ratio correlation for upward flow vertical tube under rolling motion. Based upon dimensionless groups, equations and interpolation factor, an empirical CHF correlation has been developed which is consistent with experimental data for uniformly heated tubes internally cooled by R-134 under rolling motion. Flow regime was determined through the prediction method for annular flow. Non-dimensional number and function were decided by CHF mechanism of each region. Interaction of LFD and DNB regions is taken into account by means of power interpolation which is reflected void fraction at OAF. The suggested correlation predicted the CHF Ratio with reasonable accuracy, showing an average error of -0.59 and 2.51% for RMS. Rolling motion can affect bubble motion and liquid film behavior complexly by combination of tangential and centrifugal forces and mass flow than heaving motion. Through a search of literature and a comparison of previous CHF ratio results, this work can contribute to the study of boiling heat transfer and CHF for the purpose of enhancement or reduction the CHF of dynamic motion system, such as marine reactor.

Investigations upon the indefinite rolls quality assurance in multiple regression analysis

Directory of Open Access Journals (Sweden)

Kiss, I.

2012-04-01

collectives of the foundries and the rolling mills sectors, for quality assurances of rolls as far back as phase of production, as well as in exploitation of these, what lead to, inevitably, to the quality assurance of produced laminates.

Con este trabajo se ha logrado asegurar la calidad de los cilindros de laminación, debido fundamentalmente a la aportación de una determinada composición química a los materiales cilíndricos. Esta composición química mejorada, puede desarrollar de una forma efiicaz las propiedades de explotación, donde estos cilindros de laminación podrán ser fabricados, ofreciendo mejores resultados. El trabajo se presenta de una forma científica, aportando los resultados de una investigación experimental en el área de los cilindros de laminación. Dicha investigación contiene elementos suficientes y de inmediata utilidad práctica para las empresas metalúrgicas, y así de esta forma, mejorar la calidad de los cilindros de laminación. El objetivo principal es el aumento de la durabilidad y la seguridad en la explotación. En este proceso se presenta un análisis de la composición química y de la influencia sobre las propiedades mecánicas de los cilindros de laminación indefinida. Presentamos algunas correlaciones matemáticas añadiendo una interpretación gráfica entre la dureza (en la superficie de trabajo y el cuello y la composición química. La determinación de las correlaciones dobles y triples, que son realmente útiles en la práctica de la fundición, nos permite determinar los límites de variación de la composición química, con vistas a obtener los valores óptimos de la dureza.Se podrá observar una interpretación matemática de la influencia de la composición química, sobre la dureza de estos cilindros de laminación. En este sentido, realizamos el análisis de regresión múltiple el cual puede aportar un importante instrumento estadístico para la investigación de las relaciones entre las variables. Los

NON-CENTRAL ROLLING OF FLAT WORKS WITH TAPERED THICKNESS

Directory of Open Access Journals (Sweden)

I. A. Isaevich

2010-01-01

Full Text Available The way of forming of variable shape strips with rolling in non-drive waves with rounding by the movable arbor strip is analyzed. The way of rolling with derivation of speeds of deforming instruments is offered.

Design of a Small Scale Roll to Roll Device

OpenAIRE

Pereira, Amon A; White, Edward; Kramer, Rebecca Krone

2014-01-01

In the soft robotics field, hyperelastic polymer films are used in conjunction with eutectic gallium indium to create flexible strain gages. However, rapid large scale manufacturing methods of such sensors have yet to be developed. Developing new manufacturing methods will allow for researchers to build and test new soft sensor concepts faster but also pave the way for future mass-production of these sensors for consumer or industrial consumption. One of those methods would be a Roll to...

Calculation of the Stiffness in the Roll Tensioning of the Circular Saw Blade

Directory of Open Access Journals (Sweden)

Linh Vo Tung

2016-01-01

Full Text Available The circular blade has been widely used in some projects such as cutting stone, wood and other projects. Owing to its particularity and wide use, it has an important position in cutting industry. Roll tensioning is considered as an effective method which can be used to improve the stiffness and performance of the circular saw blade. The effect of rolling position and width in the roll tensioning is obvious. In this paper the calculation of the maximum stiffness at different rolling position and width in the rolling were carried out through the finite -element. The results show that three ideal points are found. And when rolling position is Ø950mm and rolling width is 20mm, the maximum stiffness of the circular saw blade whose minimum deformation is 0.028mm is found. The roll tensioning can increase the stiffness of the saw blade. It will provide a theoretical basis and guidance for the actual production.

Finite element method analysis of surface roughness transfer in micro flexible rolling

Directory of Open Access Journals (Sweden)

Qu Feijun

2016-01-01

Full Text Available Micro flexible rolling aims to fabricate submillimeter thick strips with varying thickness profile, where the surface quality of products is mainly determined by initial workpiece surface roughness and subsequent surface asperity flattening process, which is affected by process parameters during rolling. This paper shows a 3D finite element model for flexible rolling of a 250 μm thick workpiece with reduction of 20 to 50%, and rolling phase with thinner thickness indicates a better ability to decrease the surface roughness. Four types of initial workpiece surface roughness are studied in the simulation, and the influences of process parameters, such as friction coefficient, rolling speed and roll gap adjusting speed, on surface asperity flattening of workpieces with different initial surface roughness have been numerically investigated and analysed.

Magnetoresistance of rolled-up Fe3Si nanomembranes.

Science.gov (United States)

Schumann, J; Lisunov, K G; Escoffier, W; Raquet, B; Broto, J M; Arushanov, E; Mönch, I; Makarov, D; Deneke, C; Schmidt, O G

2012-06-29

Magnetotransport of individual rolled-up Fe(3)Si nanomembranes is investigated in a broad temperature range from 4.2 K up to 300 K in pulsed magnetic fields up to 55 T. The observed magnetoresistance (MR) has the following pronounced features: (i) MR is negative in the investigated intervals of temperature and magnetic field; (ii) its magnitude increases linearly with the magnetic field in a low-field region and reveals a gradual trend to saturation when the magnetic field increases; (iii) the MR effect becomes more pronounced with increasing temperature. These dependences of MR on the magnetic field and temperature are in line with predictions of the spin-disorder model of the spin-flip s-d interaction assisted with creation or annihilation of magnons, which is expected above a certain critical temperature. Comparison of the MR features in rolled-up and planar samples reveals a substantial increase of the critical temperature in the rolled-up tube, which is attributed to a new geometry and internal strain arising in the rolled-up nanomembranes, influencing the electronic and magnetic properties of the material.

Simple roll coater with variable coating and temperature control for printed polymer solar cells

DEFF Research Database (Denmark)

Dam, Henrik Friis; Krebs, Frederik C

2012-01-01

of solution, enabling roll coating testing of new polymers where only small amounts are often available. We demonstrate the formation of >50 solar cells (each with an active area of 1 cm2) with printed metal back electrodes using as little as 0.1 mL of active layer solution. This approach outperforms spin...... coating with respect to temperature control, ink usage, speed and is directly compatible with industrial processing and upscaling....

Simulation of Bimetallic Bush Hot Rolling Bonding Process

Directory of Open Access Journals (Sweden)

Yaqin Tian

2015-01-01

Full Text Available Three-dimensional model of bimetallic bush was established including the drive roller and the core roller. The model adopted the appropriate interface assumptions. Based on the bonding properties of bimetallic bush the hot rolling process was analyzed. The optimum reduction ratio of 28% is obtained by using the finite element simulation software MARC on the assumption of the bonding conditions. The stress-strain distribution of three dimensions was research assumptions to interface deformation of rolling. At the same time, based on the numerical simulation, the minimum reduction ratio 20% is obtained by using a double metal composite bush rolling new technology from the experiment research. The simulation error is not more than 8%.

Rolls-Royce digital Rod Control System

International Nuclear Information System (INIS)

Pouillot, M.

2010-01-01

Full text of publication follows: Rolls-Royce has developed a new generation of Rod Control System, based on 40 years of experience. The fifth-generation Rod Control System (RCS) from Rolls-Royce offers a reliable, modular design with adaptability to your preferred platform, for modernization projects or new reactors. Flexible implementation provides the option for you to keep existing cabinets, which permits you to optimize installation approach. Main features for the power part: - Control Rod Drive Mechanism (CRDM) type: 3-coil. - Independent control of each sub-bank. - Each sub-bank is controlled by a cycler unit and 3 identical power racks, each including 4 identical power modules and a common power-supply module. - Coil-per-coil digital control: each power module embeds power-conversion, current-control, and current-monitoring functions for one coil. Control and monitoring are carried out by separate electronics in the module. Current is digitized and fully monitored by means of min-max templates. - A double-hold function is included: a power module assigned to a gripper will activate its coil if a fault risking to cause a reactor trip occurs. - Power modules are standardized, hot-pluggable and self-configured: a power module includes a set of parameters for each type of coil SG, MG, LC. The module recognizes the rack it is plugged in, and chooses automatically parameters to be used. Main benefits: - Reduced operational, maintenance, training, and inventory costs: standardization of power modules and integration of control and monitoring on the same PC-card lead to a drastic reduction of spare part types, and simplification of the system. - Easy maintenance: - Replacement of a power module solves nearly all failures due to current control or monitoring for a coil. It is done instantly thanks to hot-plug capability. - On the front plate of power-modules, LEDs provide useful information for diagnostic: current setpoint from cycler, output current bar

Disruption Management of Rolling Stock in Passenger Railway Transportation

NARCIS (Netherlands)

L.K. Nielsen (Lars Kjaer); G. Maróti (Gábor)

2009-01-01

textabstractThis paper deals with real-time disruption management of rolling stock in passenger railway transportation. We present a generic framework for modeling disruptions in railway rolling stock schedules. The framework is presented as an online combinatorial decision problem where the

SOFTWARE FOR COMPUTER-AIDED DESIGN OF CROSS-WEDGE ROLLING

OpenAIRE

A. A. Abramov; S. V. Medvedev

2013-01-01

The issues of computer technology creation of 3D-design and engineering analysis of metal forming processes using cross wedge rolling methods (CWR) are considered. The developed software for computer-aided design and simulation of cross-wedge rolling is described.