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Sample records for rhizobium etli final

  1. Determinants of nodulation competitiveness in Rhizobium etli. Final report for period September 30, 1996--September 29, 1999

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    Handelsman, Jo

    2000-01-04

    Nitrogen is a major limiting nutrient in crop production. Chemical fertilizers, which are used extensively to meet crop nitrogen requirements, contribute to the high energy inputs of modern agriculture and cause human health and environmental problems. Legumes and their bacterial associates have long been used in crop rotations to replenish soil nitrogen, but effective and reliable biological nitrogen fixation for beans is prevented by the lack of nodulation competitiveness of many Rhizobium strains used as inoculants. The result is that the inoculant strains will not occupy the host's nodules and no benefit will be derived from inoculation. Many indigenous soil strains of Rhizobium etli bv. phaseoli, the symbiont of bean, nodulate but fix little or no nitrogen, and therefore the nodulation competitiveness problem is significant for achieving maximum nitrogen benefit from bean crops. This project was directed toward developing an understanding of the basis of nodulation competitiveness.

  2. Regulation of transcription and activity of Rhizobium etli glutaminase A.

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    Huerta-Saquero, Alejandro; Calderón-Flores, Arturo; Díaz-Villaseñor, Andrea; Du Pont, Gisela; Durán, Socorro

    2004-08-01

    The present study determines the regulatory mechanisms that operate on Rhizobium etli glutaminase A. glsA gene expression levels were evaluated under several metabolic conditions by fusions of the glsA gene promoter and the transcriptional reporter cassette uidA2-aad. glsA expression was directly correlated to the glutaminase A activity found under the tested growth conditions, reaching its maximum level in the presence of glutamine and during exponential growth phase. Glutamine induces glsA expression. The influence of allosteric metabolites on glutaminase A activity was also determined. The purified enzyme was inhibited by 2-oxoglutarate and pyruvate, whereas oxaloacetate and glyoxylate modulate it positively. Glutaminase A is not inhibited by glutamate and is activated by ammonium. Glutaminase A participates in an ATP-consuming cycle where glutamine is continually degraded and resynthesized by glutamine synthetase (GS). GS and glutaminase A activities appear simultaneously during bacterial growth under different metabolic conditions and their control mechanisms are not reciprocal. Slight overproduction in glutaminase A expression causes a reduction in growth yield and a dramatic decrease in bacterial growth. We propose a model for regulation of glutaminase A, and discuss its contribution to glutamine cycle regulation. PMID:15279892

  3. Cloning and characterization of a thermostable xylitol dehydrogenase from Rhizobium etli CFN42

    DEFF Research Database (Denmark)

    Tiwari, Manish Kumar; Moon, Hee-Jung; Jeya, Marimuthu;

    2010-01-01

    An NAD(+)-dependent xylitol dehydrogenase from Rhizobium etli CFN42 (ReXDH) was cloned and overexpressed in Escherichia coli. The DNA sequence analysis revealed an open reading frame of 1,044 bp, capable of encoding a polypeptide of 347 amino acid residues with a calculated molecular mass of 35...

  4. Effective Symbiosis between Rhizobium etli and Phaseolus vulgaris Requires the Alarmone ppGpp

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    Moris, Martine; Braeken, Kristien; Schoeters, Eric; Verreth, Christel; Beullens, Serge; Vanderleyden, Jos; Michiels, Jan

    2005-01-01

    The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide screening for R. etli symbiotic mutants. RelA has a pivotal role in cellular physiology, as it catalyzes the synthesis of (p)ppGpp, which mediates the stringent response in bacteri...

  5. Genomic lineages of Rhizobium etli revealed by the extent of nucleotide polymorphisms and low recombination

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    González Víctor

    2011-10-01

    Full Text Available Abstract Background Most of the DNA variations found in bacterial species are in the form of single nucleotide polymorphisms (SNPs, but there is some debate regarding how much of this variation comes from mutation versus recombination. The nitrogen-fixing symbiotic bacteria Rhizobium etli is highly variable in both genomic structure and gene content. However, no previous report has provided a detailed genomic analysis of this variation at nucleotide level or the role of recombination in generating diversity in this bacterium. Here, we compared draft genomic sequences versus complete genomic sequences to obtain reliable measures of genetic diversity and then estimated the role of recombination in the generation of genomic diversity among Rhizobium etli. Results We identified high levels of DNA polymorphism in R. etli, and found that there was an average divergence of 4% to 6% among the tested strain pairs. DNA recombination events were estimated to affect 3% to 10% of the genomic sample analyzed. In most instances, the nucleotide diversity (π was greater in DNA segments with recombinant events than in non-recombinant segments. However, this degree of recombination was not sufficiently large to disrupt the congruence of the phylogenetic trees, and further evaluation of recombination in strains quartets indicated that the recombination levels in this species are proportionally low. Conclusion Our data suggest that R. etli is a species composed of separated lineages with low homologous recombination among the strains. Horizontal gene transfer, particularly via the symbiotic plasmid characteristic of this species, seems to play an important role in diversity but the lineages maintain their evolutionary cohesiveness.

  6. Covalent immobilization of recombinant Rhizobium etli CFN42 xylitol dehydrogenase onto modified silica nanoparticles

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    Zhang, Ye-Wang; Tiwari, Manish Kumar; Jeya, Marimuthu;

    2011-01-01

    Rare sugars have many applications in food industry, as well as pharmaceutical and nutrition industries. Xylitol dehydrogenase (XDH) can be used to synthesize various rare sugars enzymatically. However, the immobilization of XDH has not been performed to improve the industrial production of rare...... sugars. In this study, silica nanoparticles which have high immobilization efficiency were selected from among several carriers for immobilization of recombinant Rhizobium etli CFN42 xylitol dehydrogenase (ReXDH) and subjected to characterization. Among four different chemical modification methods to......,410 min at 40 °C and from 30 min to 450 min at 50 °C. The K(m) of ReXDH was slightly altered from 17.9 to only 19.2 mM by immobilization. The immobilized ReXDH had significant reusability, as it retained 81% activity after eight cycles of batch conversion of xylitol into L-xylulose. A∼71% conversion and a...

  7. The relaxase of the Rhizobium etli symbiotic plasmid shows nic site cis-acting preference.

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    Pérez-Mendoza, Daniel; Lucas, María; Muñoz, Socorro; Herrera-Cervera, José A; Olivares, José; de la Cruz, Fernando; Sanjuán, Juan

    2006-11-01

    Genetic and biochemical characterization of TraA, the relaxase of symbiotic plasmid pRetCFN42d from Rhizobium etli, is described. After purifying the relaxase domain (N265TraA), we demonstrated nic binding and cleavage activity in vitro and thus characterized for the first time the nick site (nic) of a plasmid in the family Rhizobiaceae. We studied the range of N265TraA relaxase specificity in vitro by testing different oligonucleotides in binding and nicking assays. In addition, the ability of pRetCFN42d to mobilize different Rhizobiaceae plasmid origins of transfer (oriT) was examined. Data obtained with these approaches allowed us to establish functional and phylogenetic relationships between different plasmids of this family. Our results suggest novel characteristics of the R. etli pSym relaxase for previously described conjugative systems, with emphasis on the oriT cis-acting preference of this enzyme and its possible biological relevance.

  8. The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase

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    Mendoza-Hernández Guillermo

    2010-10-01

    Full Text Available Abstract Background The extracellular proteome or secretome of symbiotic bacteria like Rhizobium etli is presumed to be a key element of their infection strategy and survival. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial symbiosis. To find out the possible role of secreted proteins we analyzed the extracellular proteome of R. etli CE3 in the exponential and stationary growth phases in minimal medium, supplemented with succinate-ammonium. Results The extracellular proteins were obtained by phenol extraction and identified by LC-ESI MS/MS. We identified 192 and 191 proteins for the exponential and stationary phases respectively. Using the software Signal P, we predicted signal peptides for 12.95% and 35.60% of the proteins identified in the exponential and stationary phases, respectively, which could therefore be secreted by the Sec pathway. For the exponential growth phase, we found in abundance proteins like the ribosomal proteins, toxins and proteins belonging to the group "defence mechanisms". For the stationary growth phase, we found that the most abundant proteins were those with unknown function, and in many of these we identified characteristic domains of proteases and peptidases. Conclusions Our study provided the first dataset of the secretome of R. etli and its modifications, which may lead to novel insights into the adaptive response of different stages of growth. In addition, we found a high number of proteins with unknown function; these proteins could be analyzed in future research to elucidate their role in the extracellular proteome of R. etli.

  9. Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family

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    Helppolainen, Satu H.; Nurminen, Kirsi P.; Määttä, Juha A. E.; Halling, Katrin K.; Slotte, J. Peter; Huhtala, Tuulia; Liimatainen, Timo; Ylä-Herttuala, Seppo; Airenne, Kari J.; Närvänen, Ale; Jänis, Janne; Vainiotalo, Pirjo; Valjakka, Jarkko; Kulomaa, Markku S.; Nordlund, Henri R.

    2007-01-01

    Rhizobium etli CFN42 is a symbiotic nitrogen-fixing bacterium of the common bean Phaseolus vulgaris. The symbiotic plasmid p42d of R. etli comprises a gene encoding a putative (strept)avidin-like protein, named rhizavidin. The amino acid sequence identity of rhizavidin in relation to other known avidin-like proteins is 20–30%. The amino acid residues involved in the (strept)avidin–biotin interaction are well conserved in rhizavidin. The structural and functional properties of rhizavidin were carefully studied, and we found that rhizavidin shares characteristics with bradavidin, streptavidin and avidin. However, we found that it is the first naturally occurring dimeric protein in the avidin protein family, in contrast with tetrameric (strept)avidin and bradavidin. Moreover, it possesses a proline residue after a flexible loop (GGSG) in a position close to Trp-110 in avidin, which is an important biotin-binding residue. [3H]Biotin dissociation and ITC (isothermal titration calorimetry) experiments showed dimeric rhizavidin to be a high-affinity biotin-binding protein. Its thermal stability was lower than that of avidin; although similar to streptavidin, it was insensitive to proteinase K. The immunological cross-reactivity of rhizavidin was tested with human serum samples obtained from cancer patients exposed to (strept)avidin. No significant cross-reactivity was observed. The biodistribution of the protein was studied by SPECT (single-photon emission computed tomography) imaging in rats. Similarly to avidin, rhizavidin was observed to accumulate rapidly, mainly in the liver. Evidently, rhizavidin could be used as a complement to (strept)avidin in (strept)avidin–biotin technology. PMID:17447892

  10. Pleiotropic effects of a rel mutation on stress survival of Rhizobium etli CNPAF512

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    Beullens Serge

    2008-12-01

    Full Text Available Abstract Background The rel gene of Rhizobium etli (relRet, the nodulating endosymbiont of the common bean plant, determines the cellular level of the alarmone (pppGpp and was previously shown to affect free-living growth and symbiosis. Here, we demonstrate its role in cellular adaptation and survival in response to various stresses. Results Growth of the R. etli relRet mutant was strongly reduced or abolished in the presence of elevated NaCl levels or at 37°C, compared to the wild type. In addition, depending on the cell density, decreased survival of exponentially growing or stationary phase relRet mutant cells was obtained after H2O2, heat or NaCl shock compared to the wild-type strain. Survival of unstressed stationary phase cultures was differentially affected depending on the growth medium used. Colony forming units (CFU of relRet mutant cultures continuously decreased in minimal medium supplemented with succinate, whereas wild-type cultures stabilised at higher CFU levels. Microscopic examination of stationary phase cells indicated that the relRet mutant was unable to reach the typical coccoid morphology of the wild type in stationary phase cultures. Assessment of stress resistance of re-isolated bacteroids showed increased sensitivity of the relRet mutant to H2O2 and a slightly increased resistance to elevated temperature (45°C or NaCl shock, compared to wild-type bacteroids. Conclusion The relRet gene is an important factor in regulating rhizobial physiology, during free-living growth as well as in symbiotic conditions. Additionally, differential responses to several stresses applied to bacteroids and free-living exponential or stationary phase cells point to essential physiological differences between the different states.

  11. Genetic Basis for Rhizobium etli CE3 O-Antigen O-Methylated Residues That Vary According to Growth Conditions▿

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    Ojeda, Kristylea J.; Box, Jodie M.; Noel, K. Dale

    2009-01-01

    The Rhizobium etli CE3 O antigen is a fixed-length heteropolymer with O methylation being the predominant type of sugar modification. There are two O-methylated residues that occur, on average, once per complete O antigen: a multiply O-methylated terminal fucose and 2-O methylation of a fucose residue within a repeating unit. The amount of the methylated terminal fucose decreases and the amount of 2-O-methylfucose increases when bacteria are grown in the presence of the host plant, Phaseolus ...

  12. Region 4 of Rhizobium etli Primary Sigma Factor (SigA) Confers Transcriptional Laxity in Escherichia coli

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    Santillán, Orlando; Ramírez-Romero, Miguel A.; Lozano, Luis; Checa, Alberto; Encarnación, Sergio M.; Dávila, Guillermo

    2016-01-01

    Sigma factors are RNA polymerase subunits engaged in promoter recognition and DNA strand separation during transcription initiation in bacteria. Primary sigma factors are responsible for the expression of housekeeping genes and are essential for survival. RpoD, the primary sigma factor of Escherichia coli, a γ-proteobacteria, recognizes consensus promoter sequences highly similar to those of some α-proteobacteria species. Despite this resemblance, RpoD is unable to sustain transcription from most of the α-proteobacterial promoters tested so far. In contrast, we have found that SigA, the primary sigma factor of Rhizobium etli, an α-proteobacteria, is able to transcribe E. coli promoters, although it exhibits only 48% identity (98% coverage) to RpoD. We have called this the transcriptional laxity phenomenon. Here, we show that SigA partially complements the thermo-sensitive deficiency of RpoD285 from E. coli strain UQ285 and that the SigA region σ4 is responsible for this phenotype. Sixteen out of 74 residues (21.6%) within region σ4 are variable between RpoD and SigA. Mutating these residues significantly improves SigA ability to complement E. coli UQ285. Only six of these residues fall into positions already known to interact with promoter DNA and to comprise a helix-turn-helix motif. The remaining variable positions are located on previously unexplored sites inside region σ4, specifically into the first two α-helices of the region. Neither of the variable positions confined to these helices seem to interact directly with promoter sequence; instead, we adduce that these residues participate allosterically by contributing to correct region folding and/or positioning of the HTH motif. We propose that transcriptional laxity is a mechanism for ensuring transcription in spite of naturally occurring mutations from endogenous promoters and/or horizontally transferred DNA sequences, allowing survival and fast environmental adaptation of α-proteobacteria. PMID

  13. Conservation of Plasmid-Encoded Traits among Bean-Nodulating Rhizobium Species

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    Brom, Susana; Girard, Lourdes; García-de los Santos, Alejandro; Sanjuan-Pinilla, Julio M.; Olivares, José; Sanjuan, Juan

    2002-01-01

    Rhizobium etli type strain CFN42 contains six plasmids. We analyzed the distribution of genetic markers from some of these plasmids in bean-nodulating strains belonging to different species (Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum, and Sinorhizobium fredii). Our results indicate that independent of geographic origin, R. etli strains usually share not only the pSym plasmid but also other plasmids containing symbiosis-related genes, with a similar organi...

  14. Evolution of rhizobium symbiosis

    NARCIS (Netherlands)

    Camp, Op den R.H.M.

    2012-01-01

    The evolution of rhizobium symbiosis is studied from several points of view in this thesis. The ultimate goal of the combined approaches is to unravel the genetic constrains of the symbiotic interaction. To this end the legume rhizobium symbiosis is studied in model plant species from the Papilionoi

  15. Effets de la fertilisation azotée, de l'inoculation par Rhizobium sp. et du régime des pluies sur la production de la biomasse et la teneur en azote du pois chiche

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    Lachaâl M.

    2009-01-01

    Full Text Available Effect of nitrogen fertilization, inoculation with Rhizobium sp. and precipitation on the biomass production and nitrogen content of chickpea. Water availability and soil fertility (especially soil N content are the major factors affecting chickpea yield in Mediterranean regions, characterized by low rainfall, poorly distributed and rapid mineralization of organic matter. In order to study the effect of N-fertilization and inoculation with Rhizobium on chickpea biomass production and N-content, field experiment were carried out at two stations of INRAT in northern Tunisia during two consecutive years, 2000-2001 and 2001-2002. Results showed that N-fertilization significantly increased shoot biomass and N-content while decreasing nodule mass. Inoculation with both the two Rhizobium strains resulted in nodule mass increase but the shoot N-content was not significantly affected. Regardless of the stations and treatments, rainfall decrease between the two years of the experiment significantly reduced shoot and root biomass while improving nodulation, especially at Oued Beja. However, the nodule developed under water deficit conditions was correlated with a very high decrease of the nitrogen fixed. Nevertheless, the mineral nitrogen apply in soil improved the N-content of aerial parts in the two stations, especially in waterless season.

  16. Genes encoding conserved hypothetical proteins localized in the conjugative transfer region of plasmid pRet42a from Rhizobium etli CFN42 participate in modulating transfer and affect conjugation from different donors.

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    Susana eBrom

    2015-01-01

    Full Text Available Among sequenced genomes, it is common to find a high proportion of genes encoding proteins that cannot be assigned a known function. In bacterial genomes, genes related to a similar function are often located in contiguous regions. The presence of genes encoding conserved hypothetical proteins (chp in such a region may suggest that they are related to that particular function. Plasmid pRet42a from Rhizobium etli CFN42 is a conjugative plasmid containing a segment of approximately 30 Kb encoding genes involved in conjugative transfer. In addition to genes responsible for Dtr (DNA transfer and replication, Mpf (Mating pair formation and regulation, it has two chp-encoding genes (RHE_PA00163 and RHE_PA00164 and a transcriptional regulator (RHE_PA00165. RHE_PA00163 encodes an uncharacterized protein conserved in bacteria that presents a COG4634 conserved domain, and RHE_PA00164 encodes an uncharacterized conserved protein with a DUF433 domain of unknown function. RHE_PA00165 presents a HTH_XRE domain, characteristic of DNA-binding proteins belonging to the xenobiotic response element family of transcriptional regulators. Interestingly, genes similar to these are also present in transfer regions of plasmids from other bacteria. To determine if these genes participate in conjugative transfer, we mutagenized them and analyzed their conjugative phenotype. A mutant in RHE_PA00163 showed a slight (10 times but reproducible increase in transfer frequency from Rhizobium donors, while mutants in RHE_PA00164 and RHE_PA00165 lost their ability to transfer the plasmid from some Agrobacterium donors. Our results indicate that the chp-encoding genes located among conjugation genes are indeed related to this function. However, the participation of RHE_PA00164 and RHE_PA00165 is only revealed under very specific circumstances, and is not perceived when the plasmid is transferred from the original host. RHE_PA00163 seems to be a fine-tuning modulator for conjugative

  17. Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules.

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    Rashid, M Harun-or; Young, J Peter W; Everall, Isobel; Clercx, Pia; Willems, Anne; Santhosh Braun, Markus; Wink, Michael

    2015-09-01

    Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnII) and three nodulation genes (nodA, nodC and nodD), DNA fingerprinting and phenotypic characterization. Analysis of housekeeping gene sequences and DNA fingerprints indicated that the strains belonged to three novel clades in the genus Rhizobium. In present study, a representative strain from each clade was further characterized by determination of cellular fatty acid compositions, carbon substrate utilization patterns and DNA-DNA hybridization and average nucleotide identity (ANI) analyses from whole-genome sequences. DNA-DNA hybridization showed 50-62% relatedness to their closest relatives (the type strains of Rhizobium etli and Rhizobium phaseoli) and 50-60% relatedness to each other. These results were further supported by ANI values, based on genome sequencing, which were 87-92% with their close relatives and 88-89% with each other. On the basis of these results, three novel species, Rhizobium lentis sp. nov. (type strain BLR27(T) = LMG 28441(T) = DSM 29286(T)), Rhizobium bangladeshense sp. nov. (type strain BLR175(T) = LMG 28442(T) = DSM 29287(T)) and Rhizobium binae sp. nov. (type strain BLR195(T) = LMG 28443(T) = DSM 29288(T)), are proposed. These species share common nodulation genes (nodA, nodC and nodD) that are similar to those of the symbiovar viciae.

  18. Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules.

    Science.gov (United States)

    Rashid, M Harun-or; Young, J Peter W; Everall, Isobel; Clercx, Pia; Willems, Anne; Santhosh Braun, Markus; Wink, Michael

    2015-09-01

    Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnII) and three nodulation genes (nodA, nodC and nodD), DNA fingerprinting and phenotypic characterization. Analysis of housekeeping gene sequences and DNA fingerprints indicated that the strains belonged to three novel clades in the genus Rhizobium. In present study, a representative strain from each clade was further characterized by determination of cellular fatty acid compositions, carbon substrate utilization patterns and DNA-DNA hybridization and average nucleotide identity (ANI) analyses from whole-genome sequences. DNA-DNA hybridization showed 50-62% relatedness to their closest relatives (the type strains of Rhizobium etli and Rhizobium phaseoli) and 50-60% relatedness to each other. These results were further supported by ANI values, based on genome sequencing, which were 87-92% with their close relatives and 88-89% with each other. On the basis of these results, three novel species, Rhizobium lentis sp. nov. (type strain BLR27(T) = LMG 28441(T) = DSM 29286(T)), Rhizobium bangladeshense sp. nov. (type strain BLR175(T) = LMG 28442(T) = DSM 29287(T)) and Rhizobium binae sp. nov. (type strain BLR195(T) = LMG 28443(T) = DSM 29288(T)), are proposed. These species share common nodulation genes (nodA, nodC and nodD) that are similar to those of the symbiovar viciae. PMID:26060217

  19. Elucidation of a novel lipid A α-(1,1)-GalA transferase gene (rgtF) from Mesorhizobium loti: Heterologous expression of rgtF causes Rhizobium etli to synthesize lipid A with α-(1,1)-GalA.

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    Brown, Dusty B; Muszynski, Artur; Carlson, Russell W

    2013-05-01

    An unusual α-(1,1)-galacturonic acid (GalA) lipid A modification has been reported in the lipopolysaccharide of a number of interesting Gram-negative bacteria, including the nitrogen-fixing bacteria Azospirillum lipoferum, Mesorhizobium huakuii and M. loti, the stalk-forming bacterium Caulobacter crescentus and the hyperthermophilic bacterium Aquifex aeolicus. However, the α-(1,1)-GalA transferase (GalAT) gene, which we have named RgtF, was not identified. Species of the Rhizobium genera produce lipid A with α-(1,4')-GalA but not α-(1,1)-GalA. The Rhizobium GalAT, RgtD, is the lipid A α-(1-4')-GalAT which utilizes the lipid donor dodecaprenyl-phosphate GalA (Dod-P-GalA) for GalA transfer. An additional Rhizobium GalAT, RgtE, is required for the biosynthesis of Dod-P-GalA. We predicted candidate rgtF genes in bacterial species known to produce lipid A with α-(1,1)-GalA. In order to determine the predicted rgtF gene function, we cloned the M. loti rgtF gene into an expression plasmid and introduced that plasmid into Rhizobium etli strains that do not contain the rgtF gene nor produce lipid A α-(1,1)-GalA. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis combined with NMR studies revealed that the lipid As from these rgtF-complemented strains were modified with an additional α-(1,1)-GalA attached to the proximal glucosamine. PMID:23283001

  20. Multiplication and Viability of some Rhizobium Strains to be used as Inoculants for Agricultural Biomass Production

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    Simina Neo

    2012-05-01

    Full Text Available Rhizobia are well known for their capacity to establish a symbiosis with legumes. They inhabit root nodules, where they reduce atmospheric nitrogen and make it available to the plant. Biological nitrogen fixation is an important component of sustainable agriculture, and rhizobial inoculants have been applied frequently as biofertilizers. In this review we approach the subject of legumes inoculation in order to improve the nitrogen fixing capacity. In the first part of the experiment, the Rhizobium strains were cultivated on media indicated in the literature as optimal for bacterial growing and development in laboratory conditions. Afterwards, the Rhizobium strains that have grown and accumulate biomass were tested in different conditions of pH and salinity. The biomass accumulation was determinate by spectrophotometer. The obtained values shown that the Rhizobium strains tested can be used to inoculate the legumes cultivated on acid, basic and alkaline soils. Finally, the stability in real time of two strains of Rhizobium (Rhizobium meliloti and Rhizobium japonicum mixed with different supports was evaluated during a 6- months period. The supports studied were: peat, peat and calcium carbonate, zeolite, and ceramic. The highest number of viable cells at the end of the experiment was obtained in ceramic with Rhizobium japonicum (8x105 cells/gram, and the lowest number of viable cells was obtained in zeolite with Rhizobium meliloti (1,1x103 cells/gram.

  1. Denitrification by Rhizobium meliloti

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    Rosen, A.

    1996-10-01

    Rhizobium meliloti strains were investigated for their denitrification activity as free-living cells and in nodules on lucerne (Medicago sativa) roots. They were also investigated for presence of nitrous oxide reductase (nos) activity and for genes using a nosZ probe derived from the Pseudomonas stutzeri. To decide whether R. meliloti strains used as inoculants contribute to the total denitrification activity in a lucerne ley, strains with different denitrifying capacities were used in field and laboratory experiments. The nitrate reduction activity of R. meliloti during anaerobic respiration was compared with that of a strain of Pseudomonas aeruginosa. A great diversity in the denitrification activity was found within strains of R. meliloti, and four of thirteen investigated strains showed an obvious denitrification activity. Two denitrifying bacteria were used as references, one strain each of Bradyrhizobium japonicum and P. aeruginosa. All but one of the R. meliloti strains hybridized to the PstI-fragment of the nosZ-gene from P. stutzeri. Two sizes of the hybridizing fragment, 5 and 7 kb, were noticed. Nos activity was only shown in three R. meliloti strains, and these were all characterized by a high denitrification activity. The potential denitrification activity was about 20, 40, and 80 times higher than the actual denitrification activity for lucerne, fallow, and grass, respectively. The potential denitrification activity was almost the same in lucerne and grass planted soils. Compared with the unplanted soil, the presence of lucerne roots in the soil increased the actual denitrification activity, while roots of both plant species, grass and lucerne, increased the potential denitrification activity in the soil. 32 refs, 7 figs, 1 tab

  2. Tanggapan Tanaman Kedelai terhadap Inokulasi Rhizobium

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    OKTI PURWANINGSIH; DIDIK INDRADEWA; SITI KABIRUN; DJAFFAR SHIDDIQ

    2013-01-01

    Nitrogen is one important element in plant growth. In soybean crop nitrogen requirement can bemet from the fixation of nitrogen which is the result of symbiosis between rhizobium bacteria withsoybean. A study on the relationship between soybean cultivar and rhizobium inoculation was conductedin Banguntapan Bantul Yogyakarta on regusol soils. Research aimed the responses of soybean cultivarof rhizobium inoculation. This research is a pot experiment that tested the response of 16 soybeancultiva...

  3. Polyol metabolism by Rhizobium trifolii.

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    Primrose, S. B.; Ronson, C W

    1980-01-01

    In Rhizobium trifolii 7000, the polyols myo-inositol, xylitol, ribitol, D-arabitol, D-mannitol, D-sorbital, and dulcitol are metabolized by inducible nicotinamide adenine dinucleotide-dependent polyol dehydrogenases. Five different polyol dehydrogenases were recognized: inositol dehydrogenase, specific for inositil; ribitol dehydrogenase, specific for ribitol; D-arabitol dehydrogenase, which oxidized D-arabitol, D-mannitol, and D-sorbitol; xylitol dehydrogenase, which oxidized xylitol and D-s...

  4. Tanggapan Tanaman Kedelai terhadap Inokulasi Rhizobium

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    OKTI PURWANINGSIH

    2013-08-01

    Full Text Available Nitrogen is one important element in plant growth. In soybean crop nitrogen requirement can bemet from the fixation of nitrogen which is the result of symbiosis between rhizobium bacteria withsoybean. A study on the relationship between soybean cultivar and rhizobium inoculation was conductedin Banguntapan Bantul Yogyakarta on regusol soils. Research aimed the responses of soybean cultivarof rhizobium inoculation. This research is a pot experiment that tested the response of 16 soybeancultivars of rhizobium inoculation. The sixteen cultivars consisted of 12 superior cultivars and 4 localcultivars. Before planting soybean seeds were inoculated with legin. Land used first sterilized usingautoclaves. Determination of soybean cultivars into four categories based on agronomic charactersnodule number, nodule dry weight, canopy N content, nitrogenase activity and grain yield components(seed dry weight. The study demonstrated that (1 Cultivars Anjasmara, Sibayak, Surya, Gepak yellow,Galunggung, Argomulyo and Baluran provide a response to rhizobium inoculation in the form of increasednitrogen fixation and grain yield (2 rhizobium inoculation on Tanggamus cultivars can increase nitrogenfixation but not followed by an increase in grain yield (3 rhizobium inoculation in cultivar Malabar,Seulawah and Petek not increase nitrogen fixation but can increase grain yield (4 rhizobium inoculationin cultivar Ijen, Sinabung, Wilis, Grobogan, and Garut not increase nitrogen fixation and grain yield.

  5. Rhizobium metallidurans sp. nov., a symbiotic heavy metal resistant bacterium isolated from the Anthyllis vulneraria Zn-hyperaccumulator.

    Science.gov (United States)

    Grison, Claire M; Jackson, Stephen; Merlot, Sylvain; Dobson, Alan; Grison, Claude

    2015-05-01

    A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (ChimEc512(T)) was isolated from 56 host seedlings of the hyperaccumulating Anthyllis vulneraria legume, which was on an old zinc mining site at Les Avinières, Saint-Laurent-Le-Minier, Gard, South of France. On the basis of 16S rRNA gene sequence similarities, strain ChimEc512(T) was shown to belong to the genus Rhizobium and to be most closely related to Rhizobium endophyticum CCGE 2052(T) (98.4%), Rhizobium tibeticum CCBAU 85039(T) (98.1%), Rhizobium grahamii CCGE 502(T) (98.0%) and Rhizobium mesoamericanum CCGE 501(T) (98.0%). The phylogenetic relationships of ChimEc512(T) were confirmed by sequencing and analyses of recA and atpD genes. DNA-DNA relatedness values of strain ChimEc512(T) with R. endophyticum CCGE 2052(T), R. tibeticum CCBAU 85039(T), R. mesoamericanum CCGE 52(T), Rhizobium grahamii CCGE 502(T), Rhizobium etli CCBAU 85039(T) and Rhizobium radiobacter KL09-16-8-2(T) were 27, 22, 16, 18, 19 and 11%, respectively. The DNA G+C content of strain ChimEc512(T) was 58.9 mol%. The major cellular fatty acid was C18 : 1ω7c, characteristic of the genus Rhizobium . The polar lipid profile included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine and moderate amounts of aminolipids, phospholipid and sulfoquinovosyl diacylglycerol. Although ChimEc512(T) was able to nodulate A. vulneraria, the nodC and nifH genes were not detected by PCR. The rhizobial strain was tolerant to high concentrations of heavy metals: up to 35 mM Zn and up to 0.5 mM Cd and its growth kinetics was not impacted by Zn. The results of DNA-DNA hybridizations and physiological tests allowed genotypic and phenotypic differentiation of strain ChimEc512(T) from species of the genus Rhizobium with validly published names. Strain ChimEc512(T), therefore, represents a novel species, for which the name Rhizobium metallidurans sp. nov. is proposed, with the type strain

  6. 75 FR 60820 - United States v. Adobe Systems, Inc., et al.; Proposed Final Judgment and Competitive Impact...

    Science.gov (United States)

    2010-10-01

    ... Statement, 61 FR 28891, 28894 (W.D.Mo. May 28, 1996). The Court entered an agreed-upon Final Judgment... Antitrust Division United States v. Adobe Systems, Inc., et al.; Proposed Final Judgment and Competitive.... Adobe Systems, Inc., et al., Civil Case No. 1:10- CV-01629. On September 24, 2010, the United...

  7. En route vers la nano stabilisation de CLIC faisceau principale et focalisation finale

    CERN Document Server

    Artoos, K; Guinchard, M; Hauviller, Claude; Lackner, F; CERN. Geneva. TS Department

    2008-01-01

    Pour atteindre la luminosité voulue de CLIC, la taille transversale du faisceau doit être de l?ordre du nanomètre. Ceci nécessite une stabilité vibratoire des quadripôles du faisceau principal de 1 nm et même 0.1 nm pour les doublets de la focalisation finale. La nano technologie et la nano stabilisation sont des activités qui évoluent rapidement dans l?industrie et centres de recherche pour des applications très variées comme l?électronique, l?optique, la chimie voire la médecine. Cette présentation décrit les avancées techniques nécessaires pour atteindre l?objectif de CLIC et les projets et collaborations R&D prévus pour démontrer la faisabilité de la nano stabilisation de CLIC en 2010.

  8. Evolutionary origin of rhizobium Nod factor signaling

    NARCIS (Netherlands)

    Streng, A.; Camp, Op den R.; Bisseling, T.; Geurts, R.

    2011-01-01

    For over two decades now, it is known that the nodule symbiosis between legume plants and nitrogen fixing rhizobium bacteria is set in motion by the bacterial signal molecule named nodulation (Nod) factor.1 Upon Nod factor perception a signaling cascade is activated that is also essential for endomy

  9. Optimizing Gateway™ technology (Invitrogen) to construct Rhizobium leguminosarum deletion mutants

    OpenAIRE

    Lanza Lucio, Monica; Alborno, Marcelo; Rey Navarro, Luis; Imperial Ródenas, Juan

    2010-01-01

    The study of the role of different genes in Rhizobium leguminosarum requires the generation of mutants by homologous recombination. In this communication we describe a novel approach to obtain deletion mutants of genes in Rhizobium using Gateway TM Cloning technology (Invitrogen) and a new vector (pK18-attR), both conjugative and Rhizobium specific, that carries the recombination tails of Gateway system. This tool is a new alternative to the classic approach based on cloning using rest...

  10. Evolutionary origin of rhizobium Nod factor signaling.

    Science.gov (United States)

    Streng, Arend; op den Camp, Rik; Bisseling, Ton; Geurts, René

    2011-10-01

    For over two decades now, it is known that the nodule symbiosis between legume plants and nitrogen fixing rhizobium bacteria is set in motion by the bacterial signal molecule named nodulation (Nod) factor. Upon Nod factor perception a signaling cascade is activated that is also essential for endomycorrhizal symbiosis (Fig. 1). This suggests that rhizobium co-opted the evolutionary far more ancient mycorrhizal signaling pathway in order to establish an endosymbiotic interaction with legumes. As arbuscular mycorrhizal fungi of the Glomeromycota phylum can establish a symbiosis with the fast majority of land plants, it is most probable that this signaling cascade is wide spread in plant kingdom. However, Nod factor perception generally is considered to be unique to legumes. Two recent breakthroughs on the evolutionary origin of Rhizobium Nod factor signaling demonstrate that this is not the case. The purification of Nod factor-like molecules excreted by the mycorrhizal fungus Glomus intraradices and the role of the LysM-type Nod factor receptor PaNFP in the non-legume Parasponia andersonii provide novel understanding on the evolution of rhizobial Nod factor signaling.

  11. (A structural assessment of the role of the cell surface carbohydrates of Rhizobium in the Rhizobium/legume symbiosis)

    Energy Technology Data Exchange (ETDEWEB)

    Hollingsworth, R.I.

    1991-01-01

    Research continued on the study of cell surface carbohydrates of Rhizobium. Objectives include: To characterize, at a structural level, the differences between the lipopolysaccharides of a representative number of strains from different Rhizobium species to determine which features of LPS structure are species-specific and might, therefore, be determinants of host specificity. Determine the effect(s) of nod gene induction on the structure of Rhizobium lipopolysaccharides and determine whether synthesis of a modified LPS molecule or a new surface glycoconjugate is initiated by nod gene induction. Develop a non-chemical means for rapidly screening large numbers of bacterial strains in order to determine which glycoconjugate structural features are conserved between strains of the same species. Provide the necessary structural information which, when coupled with developments in the rapidly expanding field of Rhizobium genetics, should lead to a clear understanding of the role of Rhizobium surface glycoconjugates in host/symbiont interactions. Progress is discussed.

  12. Variability among Rhizobium Strains Originating from Nodules of Vicia faba.

    Science.gov (United States)

    van Berkum, P; Beyene, D; Vera, F T; Keyser, H H

    1995-07-01

    Rhizobium strains from nodules of Vicia faba were diverse in plasmid content and serology. Results of multilocus gel electrophoresis and restriction fragment length polymorphism indicated several deep chromosomal lineages among the strains. Linkage disequilibrium among the chromosomal types was detected and may have reflected variation of Rhizobium strains in the different geographical locations from which the strains originated. An investigation of pea strains with antibodies prepared against fava bean strains and restriction fragment length polymorphism analyses, targeting DNA regions coding for rRNA and nodulation, indicated that Rhizobium strains from V. faba nodules were distinguishable from those from Pisum sativum, V. villosa, and Trifolium spp. PMID:16535075

  13. Variability among Rhizobium Strains Originating from Nodules of Vicia faba

    OpenAIRE

    van Berkum, P; Beyene, D; Vera, F. T.; Keyser, H. H.

    1995-01-01

    Rhizobium strains from nodules of Vicia faba were diverse in plasmid content and serology. Results of multilocus gel electrophoresis and restriction fragment length polymorphism indicated several deep chromosomal lineages among the strains. Linkage disequilibrium among the chromosomal types was detected and may have reflected variation of Rhizobium strains in the different geographical locations from which the strains originated. An investigation of pea strains with antibodies prepared agains...

  14. Synergy between Rhizobium phaseoli and Acidithiobacillus ferrooxidans in the Bioleaching Process of Copper

    OpenAIRE

    Xuecheng Zheng; Dongwei Li

    2016-01-01

    This study investigates the synergy of Rhizobium phaseoli and Acidithiobacillus ferrooxidans in the bioleaching process of copper. The results showed that additional R. phaseoli could increase leaching rate and cell number of A. ferrooxidans. When the initial cell number ratio between A. ferrooxidans and R. phaseoli was 2 : 1, A. ferrooxidans attained the highest final cell number of approximately 2 × 108 cells/mL and the highest copper leaching rate of 29%, which is 7% higher than that in th...

  15. Rhizobium helianthi sp. nov., isolated from the rhizosphere of sunflower.

    Science.gov (United States)

    Wei, Xuexin; Yan, Shouwei; Li, Dai; Pang, Huancheng; Li, Yuyi; Zhang, Jianli

    2015-12-01

    A Gram-stain-negative, non-spore-forming, rod-shaped and aerobic bacterium, designated Xi19T, was isolated from a soil sample collected from the rhizosphere of sunflower (Helianthus annuus) in Wuyuan county of Inner Mongolia, China and was characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate was related to species of the genus Rhizobium, sharing the greatest 16S rRNA gene sequence similarity with Rhizobium rhizoryzae J3-AN59T (98.4 %), followed by Rhizobium pseudoryzae J3-A127T (97.4 %). There were low similarities ( < 91 %) between the atpD, recA and glnII gene sequences of the novel strain and those of members of the genus Rhizobium. DNA-DNA hybridization values between strain Xi19T and the most related strain Rhizobium rhizoryzae J3-AN59T were low. The major cellular fatty acids of strain Xi19T were C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C19 : 0 cyclo ω8c. Q-10 was identified as the predominant ubiquinone and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content of strain Xi19T was 60.2 mol%. On the basis of physiological and biochemical characteristics, coupled with genotypic data obtained in this work, strain Xi19T represents a novel species of the genus Rhizobium, for which the name Rhizobium helianthi is proposed. The type strain is Xi19T ( = CGMCC 1.12192T = KCTC 23879T). PMID:26364048

  16. The beneficial effect of dual inoculation of vesicular-arbuscular mycorrhizae + rhizobium on growth of white clover

    Directory of Open Access Journals (Sweden)

    Lin, XG.

    1993-01-01

    Full Text Available Investigation on the effect of phosphorus on vesicular-arbuscular mycorrhizal infection, and dual inoculation of vesicular-arbuscular mycorrhizae + rhizobium on growth of white clover under field microplots and pot experiments was conducted on fluvo-aquic soils of semi-arid region in north China. The results showed that 60 kg P205 ha in form of superphosphate was the most favorable phosphorus level for vesicular-arbuscular mycorrhizal infection ; mycorrhizal infection, nodulation, dry weight of shoots and roots, total uptake of nitrogen, phosphorus and other elements, the final yields and recovery of phosphorus of white clover were significantly increased by vesicular-arbuscular mycorrhizal inoculation and dual inoculation with vesicular-arbuscular mycorrhizal fungi and rhizobium. The highest response of inoculation was obtained by adding fertilizer phosphorus at the level of 60 kg P205 ha in form of superphosphate.

  17. Production of nodulation factors by Rhizobium meliloti: fermentation, purification and characterization of glycolipids.

    Science.gov (United States)

    Kohring, B; Baier, R; Niehaus, K; Pühler, A; Flaschel, E

    1997-12-01

    Lipooligosaccharides, synthesized by soil bacteria of the genera Rhizobium, are known to have multifunctional effects on a wide variety of plants as signal substances in symbiosis initiation, cell response elicitation and growth regulation. These so called nodulation (Nod-) factors represent interesting biotechnological products with respect to fundamental studies of symbiotic interactions as well as for potential applications. Therefore, a batch fermentation process on a scale of 30 l has been developed by means of the Rhizobium meliloti strain R.m. 1021 (pEK327) strongly overexpressing the genes for the synthesis of Nod factors. Induction by the flavone luteolin led to growth associated production of the lipooligosaccharides. Ultrafiltration was used for separating the biomass from the filtrate containing the extracellular Nod factors. Simultaneously, ultrafiltration reduced the amount of lipophilic substances, which would otherwise interfere with processes downstream. The second separation step consisted in adsorption on XAD-2, a nonspecific hydrophobic adsorptive resin. Adsorption of Nod factors was carried out by batch operation of a stirred tank. Desorption was performed by elution with methanol in a fixed bed column. A semi-preparative reversed phase HPLC (Polygoprep 100-30 C18) was chosen as the final purification step. The Nod factors were obtained after evaporation and lyophilization. Thus, about 600 mg of Nod factors were produced from 20 l of fermentation broth. The Nod factors produced by Rhizobium meliloti R.m. 1021 (pEK327) were identified by liquid secondary ion mass spectrometry and by reversed-phase HPLC as fluorescent derivatives of 2-aminobenzamide. The biological activity of the products was demonstrated by means of the root hair deformation (HAD-) assay.

  18. Physiological role of calcium in legume-rhizobium symbiosis

    Directory of Open Access Journals (Sweden)

    Vasil’eva G.G.

    2011-12-01

    Full Text Available Literature data on the physiological role of calcium (Ca2+ in legume-rhizobium symbiosis development on initial stages - the infection and symbiotic structures formation, are generalized. The questions about the Ca2+ function in plants, special feature the formation of legume-rhizobium symbiosis and role of calcium in the interaction of two organisms are considered. Data on the interaction of ROS and Ca2+ in the development of the legume-rhizobium symbiosis and the relationship of NADPH-oxidase activity with the calcium signaling system are analyzed. The special attention is given to the role of Ca22+-spiking and calcium and calmodulin-like kinase in the initiation of plant symbiotic ways operation leads to infection and the formation of symbiotic structures.

  19. Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis.

    Science.gov (United States)

    Oliveira, Luciana Ruano; Rodrigues, Elisete Pains; Marcelino-Guimarães, Francismar Corrêa; Oliveira, André Luiz Martinez; Hungria, Mariangela

    2013-06-01

    Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia-legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p PRF81 and of the type strain of R. tropici CIAT899(T)clustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment.

  20. Towards in vivo imaging of early Rhizobium Nod factor responses

    NARCIS (Netherlands)

    Krogt, van der G.N.M.

    2006-01-01

    The goal in this thesis is to explore the possibility of live imaging of cellular events using fluorescence microscopy in combination with Green Fluorescent Protein (GFP) based reporter constructs in root hairs during theRhizobium-legume interaction. Legumes have the abilit

  1. Organic Acid Metabolism by Isolated Rhizobium japonicum Bacteroids

    Science.gov (United States)

    Stovall, Iris; Cole, Michael

    1978-01-01

    Rhizobium japonicum bacteroids isolated from soybean (Glycine max L.) nodules oxidized 14C-labeled succinate, pyruvate, and acetate in a manner consistent with operation of the tricarboxylic acid cycle and a partial glyoxylate cycle. Substrate carbon was incorporated into all major cellular components (cell wall + membrane, nucleic acids, and protein). PMID:16660386

  2. Molecular markers to study competition and diversity of Rhizobium.

    NARCIS (Netherlands)

    Sessitsch, A.

    1997-01-01

    The research described in this thesis was directed to the development of molecular identification and detection techniques for studying the ecology of Rhizobium, a nitrogen- fixing bacterium of agricultural importance. Competition of inoculant strains with indigenous microbes is a serious problem in

  3. [A structural assessment of the role of the cell surface carbohydrates of Rhizobium in the Rhizobium/legume symbiosis]. Progress report, June 1989--June 1991

    Energy Technology Data Exchange (ETDEWEB)

    Hollingsworth, R.I.

    1991-12-31

    Research continued on the study of cell surface carbohydrates of Rhizobium. Objectives include: To characterize, at a structural level, the differences between the lipopolysaccharides of a representative number of strains from different Rhizobium species to determine which features of LPS structure are species-specific and might, therefore, be determinants of host specificity. Determine the effect(s) of nod gene induction on the structure of Rhizobium lipopolysaccharides and determine whether synthesis of a modified LPS molecule or a new surface glycoconjugate is initiated by nod gene induction. Develop a non-chemical means for rapidly screening large numbers of bacterial strains in order to determine which glycoconjugate structural features are conserved between strains of the same species. Provide the necessary structural information which, when coupled with developments in the rapidly expanding field of Rhizobium genetics, should lead to a clear understanding of the role of Rhizobium surface glycoconjugates in host/symbiont interactions. Progress is discussed.

  4. Synergy between Rhizobium phaseoli and Acidithiobacillus ferrooxidans in the Bioleaching Process of Copper.

    Science.gov (United States)

    Zheng, Xuecheng; Li, Dongwei

    2016-01-01

    This study investigates the synergy of Rhizobium phaseoli and Acidithiobacillus ferrooxidans in the bioleaching process of copper. The results showed that additional R. phaseoli could increase leaching rate and cell number of A. ferrooxidans. When the initial cell number ratio between A. ferrooxidans and R. phaseoli was 2 : 1, A. ferrooxidans attained the highest final cell number of approximately 2 × 10(8) cells/mL and the highest copper leaching rate of 29%, which is 7% higher than that in the group with A. ferrooxidans only. R. phaseoli may use metabolized polysaccharides from A. ferrooxidans, and organic acids could chelate or precipitate harmful heavy metals to reduce their damage on A. ferrooxidans and promote its growth. Organic acids could also damage the mineral lattice to increase the leaching effect. PMID:26942203

  5. Synergy between Rhizobium phaseoli and Acidithiobacillus ferrooxidans in the Bioleaching Process of Copper

    Directory of Open Access Journals (Sweden)

    Xuecheng Zheng

    2016-01-01

    Full Text Available This study investigates the synergy of Rhizobium phaseoli and Acidithiobacillus ferrooxidans in the bioleaching process of copper. The results showed that additional R. phaseoli could increase leaching rate and cell number of A. ferrooxidans. When the initial cell number ratio between A. ferrooxidans and R. phaseoli was 2 : 1, A. ferrooxidans attained the highest final cell number of approximately 2 × 108 cells/mL and the highest copper leaching rate of 29%, which is 7% higher than that in the group with A. ferrooxidans only. R. phaseoli may use metabolized polysaccharides from A. ferrooxidans, and organic acids could chelate or precipitate harmful heavy metals to reduce their damage on A. ferrooxidans and promote its growth. Organic acids could also damage the mineral lattice to increase the leaching effect.

  6. Strain identification in Rhizobium by starch gel electrophoresis of isoenzymes

    DEFF Research Database (Denmark)

    Engvild, Kjeld Christensen; Nielsen, G.

    1985-01-01

    Sonieated extracts of rhizobia, especiaUy Rhizobium leguminosarum from pea and vetch, were run in horizontal starch gel electrophoresis in the cold. The rhizobia were grown on agar on a slime suppressing substrate of tryptone-yeast extract-CaCl2 with small amounts of mannitol, sorbitol and arabin......Sonieated extracts of rhizobia, especiaUy Rhizobium leguminosarum from pea and vetch, were run in horizontal starch gel electrophoresis in the cold. The rhizobia were grown on agar on a slime suppressing substrate of tryptone-yeast extract-CaCl2 with small amounts of mannitol, sorbitol...... and arabinose and other sugars as enzyme inducers. After electrophoresis the gels were separated into several slabs by a gel cutter. Each slab was stained for a particular enzyme. Among numerous enzyme systems tested we found useful variation in esterases (EC 3.1.1.1, EC 3.1.1.2), 3-hydroxybutyrate...

  7. Draft Genome Sequences of Four Novel Thermal- and Alkaline-Tolerant Egyptian Rhizobium Strains Nodulating Berseem Clover.

    Science.gov (United States)

    Shamseldin, Abdelaal; Nelson, Matthew S; Staley, Christopher; Guhlin, Joseph; Sadowsky, Michael J

    2016-01-01

    Four Rhizobium strains were isolated from berseem clover in Egypt. The symbiotically effective, salt-tolerant, strain Rhiz950 was identified as new species, Rhizobium aegypticaum sv. trifolii (USDA 7124(T)). The other three thermal- and pH-tolerant strains were identified as Rhizobium bangladeshense sv. trifolii, the type strain is USDA 7125(T). PMID:27635006

  8. Draft Genome Sequences of Four Novel Thermal- and Alkaline-Tolerant Egyptian Rhizobium Strains Nodulating Berseem Clover

    Science.gov (United States)

    Shamseldin, Abdelaal; Nelson, Matthew S.; Staley, Christopher; Guhlin, Joseph

    2016-01-01

    Four Rhizobium strains were isolated from berseem clover in Egypt. The symbiotically effective, salt-tolerant, strain Rhiz950 was identified as new species, Rhizobium aegypticaum sv. trifolii (USDA 7124T). The other three thermal- and pH-tolerant strains were identified as Rhizobium bangladeshense sv. trifolii, the type strain is USDA 7125T. PMID:27635006

  9. Root Exudates of Various Host Plants of Rhizobium leguminosarum Contain Different Sets of Inducers of Rhizobium Nodulation Genes.

    Science.gov (United States)

    Zaat, S A; Wijffelman, C A; Mulders, I H; van Brussel, A A; Lugtenberg, B J

    1988-04-01

    Rhizobium promoters involved in the formation of root nodules on leguminous plants are activated by flavonoids in plant root exudate. A series of Rhizobium strains which all contain the inducible Rhizobium leguminosarum nodA promoter fused to the Escherichia coli lacZ gene, and which differ only in the source of the regulatory nodD gene, were recently used to show that the regulatory nodD gene determines which flavonoids are able to activate the nodA promoter (HP Spaink, CA Wijffelman, E Pees, RJH Okker, BJJ Lugtenberg 1987 Nature 328: 337-340). Since these strains therefore are able to discriminate between various flavonoids, they were used to determine whether or not plants that are nodulated by R. leguminosarum produce different inducers. After chromatographic separation of root exudate constituents from Vicia sativa L. subsp. nigra (L.), V. hirsuta (L.) S.F. Gray, Pisum sativum L. cv Rondo, and Trifolium subterraneum L., the fractions were tested with a set of strains containing a nodD gene of R. leguminosarum, R. trifolii, or Rhizobium meliloti, respectively. It appeared that the source of nodD determined whether, and to what extent, the R. leguminosarum nodA promoter was induced. Lack of induction could not be attributed to the presence of inhibitors. Most of the inducers were able to activate the nodA promoter in the presence of one particular nodD gene only. The inducers that were active in the presence of the R. leguminosarum nodD gene were different in each root exudate.

  10. LysM-Type Mycorrhizal Receptor Recruited for Rhizobium Symbiosis in Nonlegume Parasponia

    NARCIS (Netherlands)

    Camp, Op den R.H.M.; Streng, A.J.; Mita, De S.; Cao, Q.; Polone, E.; Liu, W.; Ammiraju, J.S.S.; Kudrna, D.; Wing, R.; Untergasser, A.; Bisseling, T.; Geurts, R.

    2011-01-01

    Rhizobium root nodule symbiosis is generally considered to be unique for legumes. However, there is one exception and that is Parasponia. In this nonlegume, the rhizobial nodule symbiosis evolved independently and is, like in legumes, induced by rhizobium Nod factors. We used Parasponia to identify

  11. Identification and characterization of symbiotic genes on the Rhizobium leguminosarum PRE sym-plasmid.

    NARCIS (Netherlands)

    Schetgens, T.M.P.

    1986-01-01

    Bacteria of the genera Rhizobium and Bradyrhizobium are unique in their quality to form nitrogen-fixing root nodules in symbiosis with leguminous plants. In fast-growing Rhizobium bacteria the genes involved in host recognition and nodule development ( nod ) and in nitrog

  12. Studies on the expression of plasmid-borne genes in the endosymbiotic state of Rhizobium leguminosarum

    NARCIS (Netherlands)

    Krol, A.J.M.

    1982-01-01

    The subject matter of the research reported in this thesis is the role of plasmid-borne genes of Rhizobium in symbiosis and nitrogen fixation. Plasmid DNA was isolated from Rhizobium leguminosarum strain PRE and the expression of plasmid DNA in nitrogen fixing nodules was investigated by hybridizati

  13. Draft Genome Sequence of Rhizobium sp. GHKF11, Isolated from Farmland Soil in Pecan Grove, Texas

    Science.gov (United States)

    Damania, Ashish

    2016-01-01

    Rhizobium sp. GHKF11 is an organophosphate-degrading bacterial strain that was isolated from farmland soil in Pecan Grove, Texas, USA. In addition to a capacity for pesticide degradation, GHKF11 shares conserved traits with other Rhizobium spp., including heavy metal resistance and transport genes that may have significant agricultural biotechnology applications. PMID:27445376

  14. Danish Rhizobium leguminosarum strains nodulating ‘Afghanistan’ pea (Pisum sativum)

    DEFF Research Database (Denmark)

    Jensen, Erik Steen; Sørensen, Lasse Holst; Engvild, Kjeld Christensen

    1986-01-01

    A wild pea (Pisum sativum L.) native to Afghanistan normally known to be resisant to nodulation with European strains of Rhizobium leguminosarum was nodulated early and effectively in field soil in Denmark. Isolates from nodules formed effective nodules abundantly on 'Afghanistan' on reinfection...... pattern with Rhizobium leguminosarum strains isolated from a modern pea variety cultivated in the same field....

  15. 78 FR 22298 - United States v. Apple, Inc., et al.; Public Comments and Response on Proposed Final Judgment

    Science.gov (United States)

    2013-04-15

    .... Apple CEO Steve Jobs urged one Publisher Defendant to `` hrow in with Apple and see if we can all make a..., see United States v. Apple, Inc., et al., 77 FR 77094; and summaries of the terms of the...

  16. Effect of salinity on Rhizobium growth and survival.

    Science.gov (United States)

    Singleton, P W; El Swaify, S A; Bohlool, B B

    1982-10-01

    This study examines the effect of salinity on the growth and survival of Rhizobium spp. in culture media and soil. Eleven isolates from saline and nonsaline environments were compared. The growth (mean doubling time) of all strains and species tested decreased when the electrical conductivity of the culture medium (yeast extract-mannitol) was raised from 1.2 mS cm to 6.7 mS cm (15% seawater equivalent) or to 13.1 mS cm (28% seawater equivalent). Three of eleven strains failed to grow at 13.1 mS cm. Although growth was affected by salinity, four strains selected from the growth rate study could survive in extremely high concentrations of salt. Two strains with growth rates sensitive to salt and two strains with growth rates relatively unaffected by salt were inoculated into solutions with electrical conductivities of up to 43.0 mS cm (92% seawater equivalent). Not only did all four strains survive the initial osmotic shock (at 5 h after inoculation), but it was not until 27 days after inoculation that the sensitive strains exhibited a significant reduction in viable numbers. The salt-tolerant strains survived for more than 65 days with no reduction in viable counts. The interaction between soil moisture tension and soil salinity in relation to Rhizobium survival in gamma-irradiated soil was also examined. Six treatment combinations were used, ranging from -0.1 bars and 0.2 mS cm to -15 bars and 12 mS cm. Sensitive strains declined from 10 to 10 organisms per g of soil after 84 days of incubation at -15 bars and 12 mS cm. Tolerant strains survived for the same period with no loss in viable numbers. The results of these experiments indicate that many strains of Rhizobium can grow and survive at salt concentrations which are inhibitory to most agricultural legumes. The emphasis of research concerning the effects of salinity on symbiotic nitrogen fixation should, therefore, be directed to aspects of the symbiosis other than the survival of the Rhizobium spp. PMID

  17. Biochemical characterization of a fructokinase mutant of Rhizobium meliloti.

    OpenAIRE

    Gardiol, A; Arias, A.; Cerveñansky, C; Gaggero, C; Martínez-Drets, G

    1980-01-01

    A double mutant strain (UR3) of Rhizobium meliloti L5-30 was isolated from a phosphoglucose isomerase mutant (UR1) on the basis of its resistance to fructose inhibition when grown on fructose-rich medium. UR3 lacked both phosphoglucose isomerase and fructokinase activity. A mutant strain (UR4) lacking only the fructokinase activity was derived from UR3; it grew on the same carbon sources as the parent strain, but not on fructose, mannitol, or sorbitol. A spontaneous revertant (UR5) of normal ...

  18. 76 FR 68210 - United States v. George's Foods, LLC, et al.; Public Comment and Response on Proposed Final Judgment

    Science.gov (United States)

    2011-11-03

    ... United States v. George's Foods, Inc., et. al., 76 FR 38419; and summaries of the terms of the proposed... its purchases.'' IIB Philip E. Areeda, Herbert Hovenkamp, & John L. Solow, Antitrust Law 575 at 442... attorney advisor to Chairman Robert Pitofslcy. Mr. Balto is also a Senior Fellow at the Center for...

  19. Final Report: Research Study on Development of Environmental Friendly Spray-on Foam Insulation (SOFI) for the External Tank (ET)

    Science.gov (United States)

    Stuckey, James M.

    1996-01-01

    The selection and quantification of four foams using a more environmentally friendly HCFC-141b blowing agent replacing foams that used the CFC-11 blowing agent for the external tank (ET) LWT has been addressed along with problems and solutions that were encountered during verification. The effort on two lower density spray foams for the ET SLWT are presented, but predicted weight savings were not encouraging. Suggestions for possible problem solving are included along with a new approach for selecting foams for qualification as back-up foams for the foams used on the ET LWT. We investigated three resins for use as thermally sprayed coatings for corrosion prevention on metal. The best coating was obtained with a thermoplastic polyimide resin. This coating has a good chance of meeting ET requirements. Possible third generation blowing agents have been shown usable in polyurethane spray and pour foams, and solubility in isocyannate foam components are acceptable. We considered aerogels as insulation materials on space vehicles, and suggested a liner for a liquid oxygen (LOX) composite tank.

  20. 78 FR 33437 - United States v. Apple, Inc., et al.; Public Comments and Response on Proposed Final Judgment

    Science.gov (United States)

    2013-06-04

    ... in the United States (``Publisher Defendants'') conspired to raise prices of electronic books (``e... 25, 2013, see United States v. Apple, Inc., et al., 78 FR 12874; and summaries of the terms of the..., e.g., Original Response to Comments (Docket No. 81; 77 FR 44271); Penguin Response to...

  1. Effect of Rhizobium Inoculation on Phosphorus Uptake, Yield and Quality of Soybean Plants

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46 as experimental soybeans, four treatments were designed, including non-inoculated CK group and three treatment groups inoculated respectively with rhizobium strains R2, R, and Rs, to analysis the effects of rhizobium inoculation on growth status, phosphorus uptake, yield and quality of soybean plants. [Result] Inoculation with different rhizobium strains could significantly increase the biomass and phosphorus content of the roots and shoots of Dongnong 42, to be specific, rhizobium strain R2 showed the best ef- fect, followed by Rs, and those of roots were improved greater than the shoots; in- oculation with rhizobium could significantly increase the yield of Dongnong 42, and R5 showed the best effect, but the yield of Dongnong 46 was decreased; after in- oculated with rhizobium, the protein content and fat content of soybean were signifi- cantly increased, and R2 showed the best effect, but the effects on different varieties varied, to be specific, the protein content of Dongnong 42 was significantly in- creased, while the fat content of Dongnong 46 was significantly increased; different rhizobium strains inoculated to different soybean varieties showed different effects. Inoculation with suitable rhizobium can improve plant growth characteristics, promote phosphorus uptake of plants, increase soybean yield, and effectively improve soy- bean quality. [Conclusion] This study provides theoretical basis for solving the prob- lem of soil phosphorus deficiency, increasing soybean yield and improving soybean quality in the future.

  2. PENGARUH pH MEDIA PERTUMBUHAN TERHADAP KETAHANAN DARI Rhizobium sp. PADA TANAH YANG BERSIFAT ASAM

    Directory of Open Access Journals (Sweden)

    Ni Made Widyasari

    2015-02-01

    Full Text Available Telah dilakukan penelitian yang bertujuan mengetahui ketahanan Rhizobium sp. yang ditumbuhkan pada pH media pertumbuhan 7,0 dan 5,8 serta mengetahui kemampuan hidup Rhizobium sp. pada media tanah asam dengan pH 5,0 dan respon inokulum Rhizobium sp. pada tanaman kedelai secara in vivo. ATR (Acid Tolerance Responce didapatkan dengan cara menumbuhkan Rhizobium sp. dengan pH media pertumbuhan 7,0 dan ditambahkan dengan media YMB (Yeast Extract Mannitol Broth dengan pH 5,8 dan pH 7,0, diinkubasi pada suhu 280C selama 24 jam pada shaker. Penghitungan total mikroba dilakukan setiap 2 jam sekali dengan menggunakan platting method. Uji ketahanan Rhizobium sp. pada tanah asam dengan pH 5,0 secara in vitro dengan cara menghitung total bakteri dengan menggunakan platting method setiap hari selama 28 hari. Uji in vivo dilakukan dirumah kaca dengan menggunakan metoda MPN (Most Probable Number. Hasil penelitian menunjukkan Rhizobium sp. yang dikondisikan pertumbuhannya dengan pH 5,8 lebih resisten dan dapat membentuk ATR dibandingkan dengan Rhizobium sp. yang ditumbuhkan pada pH media 7,0. Pada pH 5,8 setelah 10 jam total bakteri 285 CFU/g sedangkan pada pH 7,0 total bakteri 148 CFU/g. Rhizobium sp. mampu hidup pada tanah asam dengan pH 5,0 dan membentuk ATR pada hari ke 6 dengan total bakteri 137 x 104 CFU/g, tetapi respon inokulum Rhizobium sp. pada tanah dengan pH 5,0 tidak terjadi pembentukan nodul pada tanaman kedelai dikarenakan tanaman kedelai mengalami defisiensi unsur hara.

  3. Antimicrobial activities of Rhizobium sp. strains against Pseudomonas savastanoi, the agent responsible for the olive knot disease in Algeria

    Energy Technology Data Exchange (ETDEWEB)

    Mourad, K.; Fadhila, K.; Chahinez, M.; Merien, R.; Philippe, L. de; Abdelkader, B.

    2009-07-01

    In the present investigation, six Rhizobium strains isolated from Algerian soil were checked for their antimicrobial activity against Pseudomonas savastanoi, the agent responsible for olive knot disease. Rhizobium sp. ORN 24 and ORN 83 were found to produce antimicrobial activities against Pseudomonas savastanoi. The antimicrobial activity produced by Rhizobium sp. ORN24 was precipitable with ammonium sulfate, between 1,000 and 10,000 KDa molecular weight, heat resistant but sensitive to proteases and detergents. These characteristics suggest the bacteriocin nature of the antimicrobial substance produced by Rhizobium sp. ORN24, named rhizobiocin 24. In contrast, the antimicrobial activity produced by Rhizobium sp. ORN83 was not precipitable with ammonium sulfate; it was smaller than 1,000 KDa molecular weight, heat labile, and protease and detergent resistant. These characteristics could indicate the relationship between the antimicrobial substance produced by Rhizobium sp. ORN 83 and the small bacteriocins described in other rhizobia. (Author) 51 refs.

  4. Identification and characterization of symbiotic genes on the Rhizobium leguminosarum PRE sym-plasmid.

    OpenAIRE

    Schetgens, T.M.P.

    1986-01-01

    Bacteria of the genera Rhizobium and Bradyrhizobium are unique in their quality to form nitrogen-fixing root nodules in symbiosis with leguminous plants. In fast-growing Rhizobium bacteria the genes involved in host recognition and nodule development ( nod ) and in nitrogen fixation ( nif or fix ) are located on large sym -plasmids (for recent review see e.g. Ausubel, 1984).The aim of the present investigations was to identify symbiotic genes in R.leguminosarum PRE and to study their expressi...

  5. Uji Asosiasi Bakteri Rhizobium Terseleksi dengan Leguminosa Pakan dalam Kondisi Tercekam Salin

    Directory of Open Access Journals (Sweden)

    Eny Fuskhah

    2014-04-01

    Full Text Available (Test of Association Selected Rhizobium Bacteria with Legumes in Salinity Stress ABSTRACT. The research aim was to investigate association selected rhizobium bacteria with legumes in salinity stress. Plant media was salin soil that have EC = 20.45 mmhos/cm which taken from Morosari beach, Sayung, Demak. Rhizobium isolate applied was tolerant to 12.000 ppm of NaCl that equaled to electrical conductivity of 20 mmhos/cm. The research was carried out in green house of Laboratory of Forage Science Diponegoro University Semarang. The design arranged was completely randomized design with factorial design 2 x 4 in 3 repeatations. First factor was kind of legumes, T1 = lamtoro (Leucaena leucocephala; T2 = turi (Sesbania grandiflora. and second factor was kind of rhizobium isolates, I1 = without isolate; I2 = rhizobium that was isolated from lamtoro, I3 = rhizobium that was isolated from turi; I4 = combination isolate from lamtoro and turi. The crop growth was observed up to 10 weeks of age. The parameters were 1 crops heigh; 2 sum of leaf crops; 3 fresh weight production; 4 dry weight production; 5 amount and fresh weigh of effective root nodules. The study showed the growth and production of turi in saline media of EC 20.45 mmhos/cm was higher than lamtoro. Root nodule of turi was formed, but lamtoro was not. Turi was more tolerant than lamtoro at very saline media.

  6. Portraits du dégénéré en fou, en primitif, en enfant et finalement en artiste.

    Directory of Open Access Journals (Sweden)

    Stéphane Legrand

    2004-04-01

    Full Text Available Cet article traite du concept de « dégénérescence », importé dans la psychiatrie française par Benedict-Auguste Morel dans les années 1850, et largement diffusé par la suite, dans ce champ ainsi que dans celui de la criminologie. On tente d’analyser la reconfiguration qu’impose ce concept au savoir psychiatrique en dégageant la manière dont il permet d’intégrer en un ensemble cohérent plusieurs modèles théoriques: un paradigme neurologique, une théorie de l’automatisme morbide, un certain évolutionnisme. Sur ces bases, on essaie d’établir les similitudes de fonds existant entre les conceptions psychiatrique et criminologique de la dégénérescence, en montrant qu’elles mobilisent le même réseau « structurant » d’analogies entre les figures de l’anormal (le fou, le sauvage, l’enfant, la femme, l’animal; et l’on s’efforce de montrer que ces théories promeuvent une logique dans laquelle les infractions aux différents types de normes (biologiques, sociales, morales, juridiques, psychologiques, économiques sont susceptibles de faire systématiquement référence les unes aux autres, de se traduire les unes dans les autres. Puis l’on caractérise les transformations que ce nouveau paradigme impose aux principes de la thérapeutique, de la pédagogie et de la prise en charge des malades mentaux et des déviants. Pour finir, on présente les éléments latents qui dans ce paradigme préparent et indiquent déjà sa remise en cause et son renversement à venir.This article deals with the concept of «degeneration», introduced by Benedict-Auguste Morel in the French psychiatry during the 1850s, and which widely spread afterwards, in this field as well as in the contemporary criminology. An analysis is tried of the changes imposed by this notion on the psychiatric knowledge, changes that resulted in the integration in a coherent system of three other paradigms: a neurological paradigm, a theory of

  7. Rhizobium grahamii sp. nov., from nodules of Dalea leporina, Leucaena leucocephala and Clitoria ternatea, and Rhizobium mesoamericanum sp. nov., from nodules of Phaseolus vulgaris, siratro, cowpea and Mimosa pudica.

    Science.gov (United States)

    López-López, Aline; Rogel-Hernández, Marco A; Barois, Isabelle; Ortiz Ceballos, Angel I; Martínez, Julio; Ormeño-Orrillo, Ernesto; Martínez-Romero, Esperanza

    2012-09-01

    Two novel related Rhizobium species, Rhizobium grahamii sp. nov. and Rhizobium mesoamericanum sp. nov., were identified by a polyphasic approach using DNA-DNA hybridization, whole-genome sequencing and phylogenetic and phenotypic characterization including nodulation of Leucaena leucocephala and Phaseolus vulgaris (bean). As similar bacteria were found in the Los Tuxtlas rainforest in Mexico and in Central America, we suggest the existence of a Mesoamerican microbiological corridor. The type strain of Rhizobium grahamii sp. nov. is CCGE 502(T) (= ATCC BAA-2124(T) = CFN 242(T) = Dal4(T) = HAMBI 3152(T)) and that of Rhizobium mesoamericanum sp. nov. is CCGE 501(T) (= ATCC BAA-2123(T) = HAMBI 3151(T) = CIP 110148(T) = 1847(T)). PMID:22081714

  8. Draft Genome Sequence of a Phthalate Ester-Degrading Bacterium, Rhizobium sp. LMB-1, Isolated from Cultured Soil

    OpenAIRE

    Tang, Wen-Juan; Ying ZHOU; Ye, Bang-Ce

    2015-01-01

    Rhizobium sp. LMB-1, newly isolated from greenhouse soil, can effectively degrade phthalate. Here, we present a 5.2-Mb assembly of this Rhizobium sp. genome for the first time. It may provide abundant molecular information for the transformation of phthalates.

  9. Characterization of the N2O-producing soil bacterium Rhizobium azooxidifex sp. nov.

    Science.gov (United States)

    Behrendt, Undine; Kämpfer, Peter; Glaeser, Stefanie P; Augustin, Jürgen; Ulrich, Andreas

    2016-06-01

    In the context of studying the bacterial community involved in nitrogen transformation processes in arable soils exposed to different extents of erosion and sedimentation in a long-term experiment (CarboZALF), a strain was isolated that reduced nitrate to nitrous oxide without formation of molecular nitrogen. The presence of the functional gene nirK, encoding the respiratory copper-containing nitrite reductase, and the absence of the nitrous oxide reductase gene nosZ indicated a truncated denitrification pathway and that this bacterium may contribute significantly to the formation of the important greenhouse gas N2O. Phylogenetic analysis based on the 16S rRNA gene sequence and the housekeeping genes recA and atpD demonstrated that the investigated soil isolate belongs to the genus Rhizobium. The closest phylogenetic neighbours were the type strains of Rhizobium. subbaraonis and Rhizobium. halophytocola. The close relationship with R. subbaraonis was reflected by similarity analysis of the recA and atpD genes and their amino acid positions. DNA-DNA hybridization studies revealed genetic differences at the species level, which were substantiated by analysis of the whole-cell fatty acid profile and several distinct physiological characteristics. Based on these results, it was concluded that the soil isolate represents a novel species of the genus Rhizobium, for which the name Rhizobium azooxidifex sp. nov. (type strain Po 20/26T=DSM 100211T=LMG 28788T) is proposed. PMID:27030972

  10. NopC Is a Rhizobium-Specific Type 3 Secretion System Effector Secreted by Sinorhizobium (Ensifer) fredii HH103

    Science.gov (United States)

    Medina, Carlos; Ollero, Francisco Javier; López-Baena, Francisco Javier

    2015-01-01

    Sinorhizobium (Ensifer) fredii HH103 is a broad host-range nitrogen-fixing bacterium able to nodulate many legumes, including soybean. In several rhizobia, root nodulation is influenced by proteins secreted through the type 3 secretion system (T3SS). This specialized secretion apparatus is a common virulence mechanism of many plant and animal pathogenic bacteria that delivers proteins, called effectors, directly into the eukaryotic host cells where they interfere with signal transduction pathways and promote infection by suppressing host defenses. In rhizobia, secreted proteins, called nodulation outer proteins (Nops), are involved in host-range determination and symbiotic efficiency. S. fredii HH103 secretes at least eight Nops through the T3SS. Interestingly, there are Rhizobium-specific Nops, such as NopC, which do not have homologues in pathogenic bacteria. In this work we studied the S. fredii HH103 nopC gene and confirmed that its expression was regulated in a flavonoid-, NodD1- and TtsI-dependent manner. Besides, in vivo bioluminescent studies indicated that the S. fredii HH103 T3SS was expressed in young soybean nodules and adenylate cyclase assays confirmed that NopC was delivered directly into soybean root cells by means of the T3SS machinery. Finally, nodulation assays showed that NopC exerted a positive effect on symbiosis with Glycine max cv. Williams 82 and Vigna unguiculata. All these results indicate that NopC can be considered a Rhizobium-specific effector secreted by S. fredii HH103. PMID:26569401

  11. NopC Is a Rhizobium-Specific Type 3 Secretion System Effector Secreted by Sinorhizobium (Ensifer) fredii HH103.

    Science.gov (United States)

    Jiménez-Guerrero, Irene; Pérez-Montaño, Francisco; Medina, Carlos; Ollero, Francisco Javier; López-Baena, Francisco Javier

    2015-01-01

    Sinorhizobium (Ensifer) fredii HH103 is a broad host-range nitrogen-fixing bacterium able to nodulate many legumes, including soybean. In several rhizobia, root nodulation is influenced by proteins secreted through the type 3 secretion system (T3SS). This specialized secretion apparatus is a common virulence mechanism of many plant and animal pathogenic bacteria that delivers proteins, called effectors, directly into the eukaryotic host cells where they interfere with signal transduction pathways and promote infection by suppressing host defenses. In rhizobia, secreted proteins, called nodulation outer proteins (Nops), are involved in host-range determination and symbiotic efficiency. S. fredii HH103 secretes at least eight Nops through the T3SS. Interestingly, there are Rhizobium-specific Nops, such as NopC, which do not have homologues in pathogenic bacteria. In this work we studied the S. fredii HH103 nopC gene and confirmed that its expression was regulated in a flavonoid-, NodD1- and TtsI-dependent manner. Besides, in vivo bioluminescent studies indicated that the S. fredii HH103 T3SS was expressed in young soybean nodules and adenylate cyclase assays confirmed that NopC was delivered directly into soybean root cells by means of the T3SS machinery. Finally, nodulation assays showed that NopC exerted a positive effect on symbiosis with Glycine max cv. Williams 82 and Vigna unguiculata. All these results indicate that NopC can be considered a Rhizobium-specific effector secreted by S. fredii HH103. PMID:26569401

  12. Processing parameters matching effects upon Rhizobium tropici biopolymers' rheological properties.

    Science.gov (United States)

    Pimenta, Flávia Duta; Lopes, Léa Maria de Almeida; de França, Francisca Pessôa

    2008-07-01

    The combined effects of the processing parameters upon rheological properties of biopolymers produced by Rhizobium tropici were studied as a function of the Ca(+2) ions' concentration variation, yeast extract concentration added to the medium, aeration, and agitation, maintaining the mannitol concentration in 10 g/L. The experiments were carried out using a fermenter with 20-L capacity as a reactor. All processing parameters were monitored online. The temperature [(30 +/- 1) degrees C] and pH values (7.0) were kept constant throughout the experimental time. As a statistical tool, a complete 2(3) factorial design with central point and response surface was used to investigate the interactions between relevant variables of the fermentation process: calcium carbonate concentration, yeast extract concentration, aeration, and agitation. The processing parameter setup for reaching the maximum response for rheological propriety production was obtained when applying mannitol concentration of 10.0 g/L, calcium carbonate concentration 1.0 g/L, yeast extract concentration 1.0 g/L, aeration 1.30 vvm, and agitation 800 rpm. The viscosimetric investigation of polysaccharide solutions exposed their shear-thinning behavior and polyelectrolytic feature. PMID:18437296

  13. High catalase production by Rhizobium radiobacter strain 2-1.

    Science.gov (United States)

    Nakayama, Mami; Nakajima-Kambe, Toshiaki; Katayama, Hideki; Higuchi, Kazuhiko; Kawasaki, Yoshio; Fuji, Ryujiro

    2008-12-01

    To promote the application of catalase for treating wastewater containing hydrogen peroxide, bacteria exhibiting high catalase activity were screened. A bacterium, designated strain 2-1, with high catalase activity was isolated from the wastewater of a beverage factory that uses hydrogen peroxide. Strain 2-1 was identified as Rhizobium radiobacter (formerly known as Agrobacterium tumefaciens) on the basis of both phenotypic and genotypic characterizations. Although some strains of R. radiobacter are known plant pathogens, polymerase chain reaction (PCR) analysis showed that strain 2-1 has no phytopathogenic factor. Compared with a type strain of R. radiobacter, the specific catalase activity of strain 2-1 was approximately 1000-fold. Moreover, Strain 2-1 grew faster and exhibited considerably higher catalase activity than other microorganisms that have been used for industrial catalase production. Strain 2-1 is harmless to humans and the environment and produces catalase efficiently, suggesting that strain 2-1 is a good resource for the mass production of catalase for the treatment of hydrogen peroxide-containing wastewater. PMID:19134550

  14. The isolation, enumeration, and characterization of Rhizobium bacteria of the soil in Wamena Biological Garden

    Directory of Open Access Journals (Sweden)

    SRI PURWANINGSIH

    2005-04-01

    Full Text Available The eleven soil samples have been isolated and characterized. The aims of the study were to get the pure culture and some data which described about enumeration and especially their characters in relation to the acids and bases reaction in their growth. The isolation of the bacteria use Yeast Extract Mannitol Agar medium (YEMA while the characterization by using YEMA medium mixed with Brom Thymol Blue and Congo Red indicators respectively. The results showed that eighteen isolates have been isolated which consisted of three low growing and fifteen fast growing bacteria. Two isolates were not indicated Rhizobium and sixteen were Rhizobium. Density of Rhizobium enumeration was varied which related to soil organic matter content. The enumeration bacteria in YEMA medium were in the range of 0.6 x 105 and 11.6 x 105 CFU /g soil. The highest population was found in soil sample of Wieb vegetation.

  15. Effect of Rhizobium and Mycorhiza inoculation on the nursery growth of Acacia and Teline monspessulana

    International Nuclear Information System (INIS)

    In an experiment accomplished in the tree nursery Tisquesusa located in Madrid (Cundinamarca) was evaluated the effect of the inoculation with strains selected of foreign and Indigenous rhizobium and Arbuscular mycorrhizal fungi am (Glomus folescutolum) on the growth, nitrogen fixation, and micorrization of Acacia (Acacia decurrens) and Retamo (Teline monspessulana) that they are used In soils recovery by the Corporacion Autonoma Regional de Cundinamarca CAR. The studied species presented positive response to the inoculation with rhizobium; the indigenous strain DQ6-09, isolated in Guatavita (Cundinamarca), presented the better results in Retamo and also in Acacia alone and in mixture with the foreign strain T1881. The inoculation with fungi AM increased the heights, dry weights, phosphorus content and percentage of micorrization in Acacia and Retamo. The double inoculation with fungi ma and rhizobium it did not increase the nitrogen fixing of Acacia while in Retamo was presented a positive effect with the strain DQ6-09

  16. Rhizobium pusense sp. nov., isolated from the rhizosphere of chickpea (Cicer arietinum L.).

    Science.gov (United States)

    Panday, Digvijay; Schumann, Peter; Das, Subrata K

    2011-11-01

    A novel bacterial strain, designated NRCPB10(T), was isolated from rhizosphere soil of chickpea (Cicer arietinum L.) in Pusa, New Delhi, India. The 16S rRNA gene sequence of strain NRCPB10(T) showed highest similarity (98.9 %) to that of Rhizobium radiobacter NCPPB 2437(T), followed by Rhizobium larrymoorei AF3-10(T) (97.7 %) and Rhizobium rubi IFO 13261(T) (97.4 %). Phylogenetic analysis of strain NRCPB10(T) based on the housekeeping genes recA and atpD confirmed its position as distinct from recognized Rhizobium species. Levels of DNA-DNA relatedness between strain NRCPB10(T) and R. radiobacter ICMP 5785(T), R. larrymoorei LMG 21410(T) and R. rubi ICMP 6428(T) were 51.0, 32.6 and 27.3 %, respectively. Cellular fatty acids of strain NRCPB10(T) were C(18 : 1)ω7c (58.9 %), C(16 : 0) (15.5 %), C(19 : 0) cyclo ω8c (11.5 %), iso-C(16 : 1) (5.8 %), C(16 : 0) 3-OH (4.5 %), C(16 : 1)ω7c (2.1 %) and C(18 : 0) (1.3 %). The G+C content of the genomic DNA of strain NRCPB10(T) was 59.0 mol%. Strain NRCPB10(T) did not nodulate chickpea plants or induce tumours in tobacco plants. Phenotypic and physiological properties along with SDS-PAGE of whole-cell soluble proteins differentiated strain NRCPB10(T) from its closest phylogenetic neighbours. On the basis of data from the present polyphasic taxonomic study, strain NRCPB10(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pusense sp. nov. is proposed. The type strain is NRCPB10(T) ( = LMG 25623(T) = JCM 16209(T) = NCIMB 14639(T)).

  17. Antimicrobial activity of Rhizobium sp. strains against Pseudomonas savastanoi, the agent responsible for the olive knot disease in Algeria

    Directory of Open Access Journals (Sweden)

    2009-06-01

    Full Text Available In the present investigation, six Rhizobium strains isolated from Algerian soil were checked for their antimicrobial activity against Pseudomonas savastanoi, the agent responsible for olive knot disease. Rhizobium sp. ORN 24 and ORN 83 were found to produce antimicrobial activities against Pseudomonas savastanoi. The antimicrobial activity produced by Rhizobium sp. ORN24 was precipitable with ammonium sulfate, between 1,000 and 10,000 KDa molecular weight, heat resistant but sensitive to proteases and detergents. These characteristics suggest the bacteriocin nature of the antimicrobial substance produced by Rhizobium sp. ORN24, named rhizobiocin 24. In contrast, the antimicrobial activity produced by Rhizobium sp. ORN83 was not precipitable with ammonium sulfate; it was smaller than 1,000 KDa molecular weight, heat labile, and protease and detergent resistant. These characteristics could indicate the relationship between the antimicrobial substance produced by Rhizobium sp. ORN 83 and the “small” bacteriocins described in other rhizobia.

    En la presente investigación, seis cepas de Rhizobium aisladas de suelos argelinos fueron estudiadas para conocer su actividad antimicrobiana contra Pseudomonas savastanoi, el agente causante de la tuberculosis del olivo. Rhizobium sp. ORN 24 y ORN 83 produjeron actividad antimicrobiana contra Pseudomonas savastanoi. La actividad antimicrobiana producida por Rhizobium sp. ORN 24 precipitó con sulfato amónico, tuvo un peso molecular entre 1000 y 10000 KDa, fue resistente al calor pero sensible a proteasas y detergentes. Estas características sugieren que la sustancia antimicrobial producida por Rhizobium sp. ORN 24 es la bacteriocina natural conocida como rizobiocina 24. Por el contrario, la actividad antimicrobiana producida por Rhizobium sp. ORN83 no fue precipitable con sulfato amónico, y tuvo un peso molecular menor de 1000 KDa, fue lábil al calor y resistente a detergentes y proteasas. Estas

  18. Genetic regulation of nitrogen fixation in Rhizobium meliloti.

    Science.gov (United States)

    Cebolla, A; Palomares, A J

    1994-12-01

    The soil bacterium Rhizobium meliloti fixes dinitrogen when associated with root nodules formed on its plant host, Medicago sativa (alfalfa). The expression of most of the known genes required for nitrogen fixation (nif and fix genes), including the structural genes for nitrogenase, is induced in response to a decrease in oxygen concentration. Induction of nif and fix gene expression by low oxygen is physiologically relevant because a low-oxygen environment is maintained in root nodules to prevent inactivation of the highly oxygen-sensitive nitrogenase enzyme. The genes responsible for sensing and transducing the low oxygen signal, fixL and fixJ, encode proteins (FixL and FixJ, respectively) that are homologous to a large family of bacterial proteins involved in signal transduction, the two component regulatory system proteins. The two components consist of a sensor protein, to which FixL is homologous, and a response regulator protein, to which FixJ is homologous. The sensor protein respond to an activating signal by autophosphorylating and then transferring the phosphate to its cognate response regulator protein. The phosphorylated response regulator, which is often a transcriptional activator, is then able to activate its target. A cascade model of nif and fix gene regulation in R. meliloti has been proposed, whereby FixL acts as an oxygen sensor as the initial event in the cascade and transmits this information to FixJ. FixJ, which possesses a putative helix-turn-helix DNA-binding motif, then activates transcription of the nifA and fixK genes. The nifA and fixK gene products, are transcriptional activators of at least 14 other nif and fix genes.

  19. The symbiosis between Rhizobium leguminosarum and Pisum savitum: regulation of the nitrogenase activity.

    NARCIS (Netherlands)

    Appels, M.A.

    1989-01-01

    Bacteria of the genus Rhizobium can form a symbiosis with plants of the family Leguminosae. Both bacteria and plant show considerable biochemical and morphological changes in order to develop and carry out the symbiosis. The Rhizobia induce special structures on the legumes, which are called root no

  20. Characterization of the nifA regulatory gene of Rhizobium leguminosarum PRE.

    NARCIS (Netherlands)

    Roelvink, P.W.

    1989-01-01

    This thesis describes the characterization of the nif A regulatory gene of the pea endosymbiont Rhizobiumleguminosarum PRE.Chapter I gives a general overview on the regulation of nitrogen fixation in diazotrophs, with special focus on the regulatory NifA pr

  1. Plant gene expression in early stages of Rhizobium-legume symbiosis.

    NARCIS (Netherlands)

    Gloudemans, T.

    1988-01-01

    Upon infection of the root of a leguminous plant with bacteria of the genus Rhizobium or Bradyrhizobium root nodules are formed in which symbiotic dinitrogen fixation occurs. In the host plant a set of genes is only expressed during the formation of a root nodule. These plant genes are termed noduli

  2. Effect of Rhizobium inoculation and phosphorus application on native Texas legumes grown in local soil

    NARCIS (Netherlands)

    Osman, E.; Muir, J.P.; Elgersma, A.

    2002-01-01

    Greenhouse experiments were conducted to determine the effects of Rhizobium inoculation and addition of phosphorus to soil on the productivity and quality of the Texas range legumes Desmanthus illinoiensis (Michx.) MacMill. ex B.L. Rob. & Fernald, Desmanthus velutinus Scheele, Desmanthus virgatus (L

  3. Genome of Rhizobium sp. UR51a, Isolated from Rice Cropped in Southern Brazilian Fields.

    Science.gov (United States)

    de Souza, Rocheli; Sant'Anna, Fernando Hayashi; Ambrosini, Adriana; Tadra-Sfeir, Michele; Faoro, Helisson; Pedrosa, Fabio Oliveira; Souza, Emanuel Maltempi; Passaglia, Luciane M P

    2015-01-01

    Rhizobium sp. UR51a is a Gram-negative bacterium isolated from roots of rice plants, and it presents plant growth-promoting abilities. The nutrient uptake in rice plants inoculated with UR51a was satisfactory. The genome of strain UR51a is composed of 5,233,443-bp and harbors 5,079 coding sequences. PMID:25838497

  4. Genome of Rhizobium sp. UR51a, Isolated from Rice Cropped in Southern Brazilian Fields

    OpenAIRE

    de Souza, Rocheli; Sant’Anna, Fernando Hayashi; Ambrosini, Adriana; Tadra-Sfeir, Michele; Faoro, Helisson; Pedrosa, Fabio Oliveira; Souza, Emanuel Maltempi; Passaglia, Luciane M. P.

    2015-01-01

    Rhizobium sp. UR51a is a Gram-negative bacterium isolated from roots of rice plants, and it presents plant growth-promoting abilities. The nutrient uptake in rice plants inoculated with UR51a was satisfactory. The genome of strain UR51a is composed of 5,233,443-bp and harbors 5,079 coding sequences.

  5. Rhizoplane colonisation of peas by Rhizobium leguminosarum bv. viceae and a deleterious Pseudomonas putida

    NARCIS (Netherlands)

    Berggren, I.; Alstrom, S.; Vuurde, van J.W.L.; Martensson, A.M.

    2005-01-01

    Pseudomonas putida strain angstrom 313, a deleterious rhizosphere bacterium, reduced pea nitrogen content when inoculated alone or in combination with Rhizobium leguminosarum bv. viceae on plants in the presence of soil under greenhouse conditions. When plants were grown gnotobiotically in liquid me

  6. Genome Sequence of Rhizobium lupini HPC(L) Isolated from Saline Desert Soil, Kutch (Gujarat)

    OpenAIRE

    Agarwal, Leena; Hemant J. Purohit

    2013-01-01

    The Rhizobium lupini strain HPC(L) was isolated from saline desert soil. It grows on minimal media supplemented with CaCO3 as a carbon source. It can also grow under both oligotrophic and heteroptrophic conditions. We report the annotated genome sequence of this strain in a 5.27-Mb scaffold.

  7. The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.

    Science.gov (United States)

    Oliveira, Luciana Ruano; Marcelino, Francismar Corrêa; Barcellos, Fernando Gomes; Rodrigues, Elisete Pains; Megías, Manuel; Hungria, Mariangela

    2010-08-01

    Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263-270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.

  8. Effects of Rhizobium inoculation on Trifolium resupinatum antioxidant system under sulfur dioxide pollution

    Directory of Open Access Journals (Sweden)

    Ladan Bayat

    2014-01-01

    Full Text Available Introduction: Plant growth stimulating rhizobacteria are beneficial bacteria that can cause resistance to various stresses in plants. One of these stresses is SO2 air pollution. SO2 is known as a strong damaging air pollutant that limits growth of plants. The aim of this study is evaluation of the effects of bacterial inoculation with native and standard Rhizobium on Persian clover root growth and antioxidants activity and capacity under air SO2 pollution. Materials and methods: In this study, 31 days plants (no-inoculated and inoculated with two strains of Rhizobium exposed to the different concentrations of SO2 (0 as a control, 0.5, 1, 1.5 and 2 ppm for 5 consecutive days and 2 hours per day. Results: Results showed different concentrations of SO2 had a significant effect on Persian clover root weight and antioxidant system. Increasing SO2 stress decreased root fresh and dry weight and antioxidant capacities (IC50 and increased antioxidant activities (I% of Persian clover leaves significantly in comparison to the control plants (under 0 ppm and increased SOD, CAT and GPX activity. Inoculation of Persian clover plants with native and standard Rhizobium increased root weight and did not show a significant effect on antioxidants activity and capacity, but interaction between Rhizobium inoculation and SO2 treatment reduced significantly the stress effects of high concentration of SO2 on root growth and antioxidants activity and capacity. In fact, level of this change of root growth and antioxidant system under SO2 pollution stress in inoculated plants was lower than in the non-inoculated plants. Discussion and conclusion: As a result, an increase in SO2 concentration caused a decrease in root weight, increase in antioxidants activity and capacity of Persian clover. Inoculation with Rhizobium strains could alleviate the effect of SO2 pollution on antioxidant system by effects on root growth.

  9. Inoculação com Rhizobium e aplicação de nitrogênio em amendoim Comparison among Rhizobium strains inoculations and nitrogen applications on peanut, in field conditions

    Directory of Open Access Journals (Sweden)

    Antonio Roberto Giardini

    1985-01-01

    Full Text Available Existe, nas nossas condições, uma população autóctone de Rhizobium capaz de nodular o amendoim (Arachis hypogaea L., mas pouco se sabe da contribuição do nitrogênio fixado para esta planta. Foram conduzidos dois ensaios no campo, em solo de baixa fertilidade, um no período "da seca" e outro no "das águas", comparando o crescimento e a produção de plantas de amendoim inoculado com Rhizobium selecionado, com o de plantas noduladas pela população autóctone, adubadas ou não com nitrogênio. A nodulação das plantas inoculadas foi semelhante à observada nos tratamentos não inoculados, com ou sem nitrogênio. Na fase final do ciclo das plantas, houve maior acúmulo e maior taxa de absorção diária de nitrogênio nos tratamentos inoculados ou com adubação nitrogenada, do que no controle sem inoculação e sem nitrogênio. No ensaio da seca, não houve aumento de produção devido à adubação nitrogenada, ou à inoculação. No ensaio das águas, houve resposta à aplicação de nitrogênio no plantio. Os resultados de produção não foram coerentes com os da marcha de absorção de N. A produção de ensaio das águas foi equivalente a 3.400 kg/ha para o tratamento sem nitrogênio e sem inoculação.Two field experiments were carried out with peanut in the same area on a limed and fertilized "cerrado soil" (originally acidic and low fertility. The first experiment was carried out in the autumn/winter (dry season, and the second one in the subsequent spring/summer (wet season, in Campinas, State of São Paulo, Brazil. Plant development and production of inoculated (three Rhizobium strains and nitrogen fertilized treatments (at planting 25 and 45 days after planting were compared with non-inoculated and non-N-fertilized control. Nodulation of inoculated plants was similar to those of non-inoculated, with or without nitrogen. Greater accumulations, and rates for average daily uptake of nitrogen were observed for inoculated as

  10. Rhizobium promotes non-legumes growth and quality in several production steps: towards a biofertilization of edible raw vegetables healthy for humans.

    Directory of Open Access Journals (Sweden)

    Paula García-Fraile

    Full Text Available The biofertilization of crops with plant-growth-promoting microorganisms is currently considered as a healthy alternative to chemical fertilization. However, only microorganisms safe for humans can be used as biofertilizers, particularly in vegetables that are raw consumed, in order to avoid sanitary problems derived from the presence of pathogenic bacteria in the final products. In the present work we showed that Rhizobium strains colonize the roots of tomato and pepper plants promoting their growth in different production stages increasing yield and quality of seedlings and fruits. Our results confirmed those obtained in cereals and alimentary oil producing plants extending the number of non-legumes susceptible to be biofertilized with rhizobia to those whose fruits are raw consumed. This is a relevant conclusion since safety of rhizobia for human health has been demonstrated after several decades of legume inoculation ensuring that they are optimal bacteria for biofertilization.

  11. Chemical characterization of the gels produced by the diazotrophic bacteria Rhizobium tropici and Mesorhizobium sp; Caracterizacao quimica dos geis produzidos pelas bacterias diazotroficas Rhizobium tropici e Mesorhizobium sp.

    Energy Technology Data Exchange (ETDEWEB)

    Monteiro, Nilson Kobori [Departamento de Engenharia e Tecnologia de Alimentos, Instituto de Biociencias, Letras e Ciencias Exatas, Universidade Estadual Paulista, Sao Jose do Rio Preto - SP (Brazil); Aranda-Selverio, Gabriel; Exposti, Diego Tadeu Degli; Silva, Maria de Lourdes Corradi da [Departamento de Fisica, Quimica e Biologia, Faculdade de Ciencias e Tecnologia, Universidade Estadual Paulista, Presidente Prudente - SP (Brazil); Lemos, Eliana Gertrudes Macedo; Campanharo, Joao Carlos [Departamento de Tecnologia, Faculdade de Ciencias Agrarias e Veterinaria, Universidade Estadual Paulista, Jaboticabal - SP (Brazil); Silveira, Joana Lea Meira [Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Parana, Curitiba - PR (Brazil)

    2012-07-01

    The exopolysaccharides with characteristics of gel produced by Rhizobium tropici (EPSRT) and Mesorhizobium sp (EPSMR) are acidic heteropolysaccharide composed mainly of glucose and galactose in a molar ratio of 4:1 and 5:1 respectively, with traces of mannose ({approx} 1%). Chemical analysis showed the presence of uronic acid, pyruvate and acetyl-substituents in the structures of both polymers. Experiments of gel permeation chromatography and polyacrylamide gel electrophoresis showed that EPSRT and EPSMR are homogeneous molecules with low grade of polydispersity. The EPS were characterized using spectroscopic techniques of FT-IR, {sup 1}H and {sup 13}C-NMR. (author)

  12. Interactions route-tram : Analyse du risque et des difficultés perçues entre conducteurs de tramways et autres usagers de la voirie. Rapport final

    OpenAIRE

    PARAN, Françoise; Delhomme, Patricia; CASTANIER, Carole

    2011-01-01

    L'objectif de ce projet est d'examiner, en France, les interactions entre les usagers de la voirie et le tramway tant du point de vue des traminots (étude 1) que des usagers (étude 2) afin de faire des recommandations pour améliorer la sécurité. Une première étude par entretiens semi-directifs a été menée auprès de 30 traminots de 10 agglomérations. Il s'est agi d'explorer les difficultés qu'ils rencontrent en conduisant le tram, les stratégies qu'ils mettent en oeuvre et les suggestions pour...

  13. Rhizobium sp. Degradation of Legume Root Hair Cell Wall at the Site of Infection Thread Origin

    OpenAIRE

    Ridge, Robert W.; Rolfe, Barry G.

    1985-01-01

    Using a new microinoculation technique, we demonstrated that penetration of Rhizobium sp. into the host root hair cell occurs at 20 to 22 h after inoculation. It did this by dissolving the cell wall maxtrix, leaving a layer of depolymerized wall microfibrils. Colony growth pressure “stretched” the weakened wall, forming a bulge into an interfacial zone between the wall and plasmalemma. At the same time vesicular bodies, similar to plasmalemmasomes, accumulated at the penetration site in a man...

  14. Effect of Light and Organic Acids on Oxygen Uptake by BTAi 1, a Photosynthetic Rhizobium

    OpenAIRE

    Wettlaufer, S. H.; Hardy, R. W. F.

    1992-01-01

    A photosynthetic rhizobium, strain BTAi 1, was cultured ex planta to investigate its photosynthetic-respiratory system and the response of this interactive system to light quantity and quality and to the addition of organic acids. Oxygen uptake, as measured with an oxygen electrode, is diminished upon illumination, with the amount of decrease related to light intensity. This oxygen-sparing effect is correlated with the wavelengths of light that are associated with bacteriochlorophyll absorban...

  15. Transmissible Resistance to Penicillin G, Neomycin, and Chloramphenicol in Rhizobium japonicum1

    Science.gov (United States)

    Cole, Michael A.; Elkan, Gerald H.

    1973-01-01

    The genetic basis for resistance to a number of antibiotics was examined in Rhizobium japonicum. Resistance to penicillin G, neomycin, and chloramphenicol appears to be mediated by an extrachromosomal element similar to that found in the Enterobacteriaceae. Resistance to these antibiotics was eliminated from cells by treatment with acridine orange, and resistance to all three antibiotics could be transferred en bloc to Agrobacterium tumefaciens under conditions excluding transformation or transduction as possible genetic mechanisms. PMID:4491197

  16. Efficacy of micronutrients in influencing growth behavior of Rhizobium of Pigeonpea (Cajanus cajan L. [Millsp.])

    OpenAIRE

    Sonam Paliya; A.N. Tikle; Tessy Thomas

    2014-01-01

    he present investigation was conducted to observe the effect of various concentrations (0.1 to 1.5 gm/100ml) of micronutrients (Zn, Mn, B, Cu, Mo, Fe) with yeast extract mannitol media on agriculturally important Rhizobium species isolated from root nodules of Cajanus cajan and to determine the effect of multi-micronutrient yeast extract mannitol media prepared of maximum growth concentrations of micronutrients on rhizobial growth. Concentrati...

  17. Production of Poly &beta-Hydroxybutyric Acid (PHB) by Rhizobium elti and Pseudomonas stutzeri

    OpenAIRE

    Elsayed B. Belal

    2013-01-01

    The amount of chemosynthetic plastic waste increases every year and exact time for its degradation is unknown. Two poly &beta-Hydroxybutyric producing bacteria from different microbial sources were isolated and characterized for their morphological, biochemical properties. Based on their 16S rDNA, they were identified as Rhizobium elti E1 and Pseudomonas stutzeri E114. The bacterial strains were screened for PHB production and compared for the intensity of fluorescence using Nile red staining...

  18. Industrial wastewater as raw material for exopolysaccharide production by Rhizobium leguminosarum

    OpenAIRE

    Mohamed Sellami; Tomasz Oszako; Nabil Miled; Faouzi Ben Rebah

    2015-01-01

    The objective of this study was to evaluate the exopolysaccharide (EPS) production by Rhizobium leguminosarum cultivated in wastewater generated by oil companies (WWOC1 and WWOC2) and fish processing industry (WWFP). The results obtained in Erlenmeyer flasks indicated that the rhizobial strain grew well in industrial wastewater. Generally, wastewater composition affected the growth and the EPS production. WWFP allowed good bacterial growth similar to that obtained with the standard medium (YM...

  19. Role of Dual Inoculation of Rhizobium and Arbuscular Mycorrhizal (AM Fungi on Pulse Crops Production

    Directory of Open Access Journals (Sweden)

    Erneste HAVUGIMANA

    2016-01-01

    Full Text Available Legume crops are useful as human and animal feed, wood energy, and as soil-improving components of agricultural and agro forestry systems through its association with bio-fertilizers. The later have a potential environment friendly inputs that are supplemented for proper plant growth. Bio-fertilizers are preparations containing living cells of microorganisms that help crop plants in the uptake of nutrients by their interactions in the rhizosphere. Arbuscular mycorrhizal (AM fungi are beneficial symbionts for plant growth. They are associated with higher plants by a symbiotic association and benefit plants in the uptake of phosphorus nutrients, production of growth hormones, increase of proteins, lipids and sugars levels, helps in heavy metal binding, salinity tolerance and disease resistance. In nature symbiotic association of Rhizobium and leguminous plants fixes atmospheric nitrogen. Indeed, research has proved that the association of mycorrhizae fungi and Rhizobium, with pulse crops, increased the beneficial aspects comparatively more than their single associations with the host plants. This review focuses on the role of dual inoculation of AM fungi and Rhizobium on different pulse crops.

  20. NPR1 protein regulates pathogenic and symbiotic interactions between Rhizobium and legumes and non-legumes.

    Directory of Open Access Journals (Sweden)

    Smadar Peleg-Grossman

    Full Text Available BACKGROUND: Legumes are unique in their ability to establish symbiotic interaction with rhizobacteria from Rhizobium genus, which provide them with available nitrogen. Nodulation factors (NFs produced by Rhizobium initiate legume root hair deformation and curling that entrap the bacteria, and allow it to grow inside the plant. In contrast, legumes and non-legumes activate defense responses when inoculated with pathogenic bacteria. One major defense pathway is mediated by salicylic acid (SA. SA is sensed and transduced to downstream defense components by a redox-regulated protein called NPR1. METHODOLOGY/PRINCIPAL FINDINGS: We used Arabidopsis mutants in SA defense pathway to test the role of NPR1 in symbiotic interactions. Inoculation of Sinorhizobium meliloti or purified NF on Medicago truncatula or nim1/npr1 A. thaliana mutants induced root hair deformation and transcription of early and late nodulins. Application of S. meliloti or NF on M. truncatula or A. thaliana roots also induced a strong oxidative burst that lasted much longer than in plants inoculated with pathogenic or mutualistic bacteria. Transient overexpression of NPR1 in M. truncatula suppressed root hair curling, while inhibition of NPR1 expression by RNAi accelerated curling. CONCLUSIONS/SIGNIFICANCE: We show that, while NPR1 has a positive effect on pathogen resistance, it has a negative effect on symbiotic interactions, by inhibiting root hair deformation and nodulin expression. Our results also show that basic plant responses to Rhizobium inoculation are conserved in legumes and non-legumes.

  1. Comparative effectiveness of different Rhizobium sp. for improving growth and yield of maize (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Ijaz Mehboob, Zahir Ahmad Zahir, Muhammad Arshad, Muhammad Khalid

    2012-05-01

    Full Text Available During the last couple of decades, it has been demonstrated that rhizobia can associate with roots of non-legumes also without forming true nodules, and can promote their growth by using one or more of the direct or indirect mechanisms of actions. This work examines the growth and yield responses of maize to inoculation with different species of rhizobia, isolated from the root nodules of chickpea (Cicer arietinum L., lentil (Lens culinaris M. and mung bean (Vigna radiata L. in pots and fields. Twenty isolates of rhizobia were isolated from root nodules each of mung bean, lentil and chickpea and were screened under axenic conditions. On the basis of their promising performance under axenic conditions, nine most efficient isolates (three from each legume host were selected, characterized and further evaluated for their growth promoting activities by conducting pot and field experiments. Results of pot experiment revealed that maximum increase in grain yield, 1000 grain weight, N, P and K uptake (up to 47.89, 54.52, 73.46, 84.66 and 59.19% by CRI28, respectively, over un-inoculated control was produced by the isolate of Mesorhizobium ciceri. Whereas, maximum improvement in rest of the parameters was caused by the isolates of Rhizobium phaseoli (i.e. fresh biomass, straw yield and root length up to 36.30% by A18, 25.46% by S6 and 81.89% by A18, respectively over un-inoculated control. Rhizobium leguminosarum isolates came out to be the least effective among the species tested. Similarly, all the selected isolates improved the growth and yield attributing parameters in fields as well but with varying capacity compared with un-inoculated control. The selected isolates of Mesorhizobium ciceri and Rhizobium phaseoli again remained superior compared to the isolates of Rhizobium leguminosarum under field conditions. The results of this study imply that rhizobium species had potential to promote growth and yield of maize but this technology should be

  2. Synergism of VAM and Rhizobium on production and metabolism of IAA in roots and root nodules of Vigna mungo.

    Science.gov (United States)

    Chakrabarti, Jayanta; Chatterjee, Sabyasachi; Ghosh, Sisir; Chatterjee, Narayan Chandra; Dutta, Sikha

    2010-09-01

    Mature and healthy root nodules of Vigna mungo appeared to contain higher amount of indole-acetic acid (IAA) than non-nodulated roots. Dual effect of VAM fungus, Glomus fasciculatum and the nitrogen-fixing bacteria, Rhizobium sp. on the nodulation of roots of V. mungo was studied. It was recorded that the roots which were inoculated simultaneously with both the symbionts i.e., G. fasciculatum and Rhizobium exhibited greater amount of IAA production than the non-inoculated roots. A tryptophan pool present in the mature nodules and young leaves might serve as a precursor for IAA production in the roots and in the nodules. Activity of IAA-metabolizing enzymes, such as IAA oxidase, peroxidase, and polyphenol oxidase was investigated which indicates the active metabolism of IAA in roots and nodules. The Rhizobium symbiont isolated from fresh nodules of V. mungo produced significant amount of IAA under in vitro condition when tryptophan was added to the medium as precursor. Present study represents some beneficial effects of Rhizobium and G. fasciculatum on the production and metabolism of IAA in roots and nodules of V. mungo. The important physiological implication of the study on IAA production and its metabolism in Rhizobium-Legume-VAM tripartite symbiosis is certainly representing a new approach to satisfy the hormonal balance in the host plant.

  3. Diversity of Rhizobium-Phaseolus vulgaris symbiosis: Overview and perspectives

    International Nuclear Information System (INIS)

    Common bean (Phaseolus vulgaris) has become a cosmopolitan crop, but was originally domesticated in the Americas and has been grown in Latin America for several thousand years. Consequently an enormous diversity of bean nodulating bacteria have developed and in the centers of origin the predominant species in bean nodules is R. etli. In some areas of Latin America, inoculation, which normally promotes nodulation and nitrogen fixation is hampered by the prevalence of native strains. Many other species in addition to R. etli have been found in bean nodules in regions where bean has been introduced. Some of these species such as R. leguminosarum bv. phaseoli, R. gallicum bv. phaseoli and R. giardinii bv. phaseoli might have arisen by acquiring the phaseoli plasmid from R. etli. Others, like R. trap id, are well adapted to acid soils and high temperatures and are good inoculants for bean under these conditions. The large number of rhizobia species capable of nodulating bean supports that bean is a promiscuous host and a diversity of bean-rhizobia interactions exists. Large ranges of dinitrogen fixing capabilities have been documented among bean cultivars and commercial beans have the lowest values among legume crops. Knowledge on bean symbiosis is still incipient but could help to improve bean biological nitrogen fixation. (author)

  4. Colonization and nitrogenase activity of Triticum aestivum (cv. Baccross and Mahdavi) to the dual inoculation with Azospirillum brasilense and Rhizobium meliloti plus 2,4-D.

    Science.gov (United States)

    Mehry, Askary; Akbar, Mostajeran; Giti, Emtiazi

    2008-06-15

    The potential enhancement of root colonization and nitrogenase activity of wheat cultivars (Baccross and Mahdavi) was studied with application of two Azospirillum brasilense strains (native and Sp7) co-inoculated with two Rhizobium meliloti strains (native and DSMZ 30135). The results indicated that the colonization was different due to the strains and cultivars of wheat were used. Native A. brasilense colonized wheat root better than Sp7 strain. However, Baccross cv. reacted better with native Azospirillum compared to Mahdavi cv. which reacted better with Sp7. When plants inoculated with dual inoculants (SP7 with standard Rhizobium), the colonization of Azospirillum were increased significantly (from 1.67 x 10(5) to 22 x 10(5) cfu g(-1) FW for Baccras cv. and 3.67 x 10(5) to 26 x 10(5) cfu g(-1) FW for Mahdavi cultivar). When the standard Rhizobium as co-inoculants changed to the native Rhizobium, the colonization of Azospirillum was higher when compared to the single inoculants but was almost the same when compared to the standard Rhizobium. When the standard or native strains of Rhizobium used as single inoculation of wheat roots, the number of Rhizobium in the wheat roots were not changed significantly. However, when plants co-inoculated with Rhizobium and Azospirillum, the colonization of Rhizobium was increased. Co-inoculation of standard strain of R. melilot with A. brasilense Sp7 showed that the colonization of Rhizobium were increased from 0.67 x 10(5) to 21 x 10(5) cfu g(-1) FW for Baccross cv. and 0.33 x 10(5) to 18 x 10(5) cfu g(-1) FW for Mahdavi cv. This behavior was the same when inoculation of Rhizobium was happened with the native one. In dual inoculation, the highest nitrogenase activity was measured in combination of the local strains (native A. brasilense with the native R. meliloti) and the lower one belongs to the combination of standard strains (Sp7 with standard R. meliloti). The difference in nirtogenase activity for different cultivars of

  5. Fertilisation de la courge de plein champ : test de différentes doses et de 2 types d'engrais - rapport final 2005

    OpenAIRE

    Védie, Hélène

    2006-01-01

    La fertilisation en maraîchage biologique est sans aucun doute encore un vaste domaine à explorer. Le manque de références sur les besoins des cultures d’une part, et sur les possibilités de fournitures du sol par minéralisation d’autre part, amène bien souvent les producteurs à « naviguer à vue » en utilisant des doses standards, qui ne reposent pas sur des bases agronomiques éprouvées. Les suivis réalisés par le GRAB sur aubergine en 2003 et melon en 2004 montrent qu’il est possible de d...

  6. Symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34

    Institute of Scientific and Technical Information of China (English)

    Fengqing Li; Bihe Hou; Guofan Hong

    2008-01-01

    NolR is a reguiator of noduiation genes present in Rhizobium and Sinorhizobium. However, the mechanism by which NolR participates in the inducible transcription ofnoduiation genes remains unclear. To investigate whether there are other factors regulating the function of NoIR, an insertion mutant of NolR in Rhizobium leguminosarum strain 8401, which lacks the symbiotic plasmid, was constructed by homologous recombination. We investigated the effects of NolR inactivation on the expression of nodulation genes. Three inducible nodulation genes (nodA, nodF and nodM) were expressed constitutively in NoiR-mutant, MRl14. Our results suggested that the symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium ieguminosarum A34. In addition, MRl14 has provided a useful tool for further study of molecular interactions between NolR and other factors.

  7. Biologie, écologie et pêche des lamproies migratrices (agnathes amphihalins): rapport final - Troisième tranche fonctionnelle -

    OpenAIRE

    TAVERNY C.; Urdaci, M.; Elie, A.M.; BEAULATON L.; Ortusi, I.; Daverat, F.; ELIE P.

    2005-01-01

    The pattern of growth larval habitat Petromyzon marinus et Lampetra sp..preferences was examined in 4th order rivers Livenne, Cère, Dronne and in the 6th order river Dordogne (modules from 279 to 2,5 m3/s). From shallow waters to deepwater areas, the same dredging technique was used while diving to catch burrowing larvae. Sampled sections of rivers were chosen downstream of one spawning ground at least. Larvae aggregated at sites presenting a soft river bed in deep macrohabitats or shallow wa...

  8. Influence of Phosphate on the Growth and Nodulation Characteristics of Rhizobium trifolii†

    OpenAIRE

    Leung, Kamtin; Bottomley, Peter J.

    1987-01-01

    The growth and nodulating characteristics of Rhizobium trifolii 6 and 36 differed under different external phosphate conditions. Under growth conditions designed to deplete the internal phosphate content of the rhizobia, strain 6 maintained a generation time of 5 h during the exponential phase over two cycles of growth in phosphate-depleted medium. In contrast, the generation time of strain 36 was extended from 3.5 to 9.8 h over two cycles of phosphate-depleted growth, although the organism e...

  9. Parallel variation in isoenzyme and nitrogen fixation markers in a Rhizobium population

    DEFF Research Database (Denmark)

    Engvild, K.C.; Jensen, E.S.; Skøt, L.

    1990-01-01

    Twenty isolates of Rhizobium leguminosarum bv. viceae were isolated at random from one field and examined for symbiotic plasmid fragment length polymorphisms and for isoenzyme patterns. The latter are most probably chromosome markers. With one exception both methods separated the isolates...... into the same 13 different groups. The largest group was represented 7 times according to isoenzymes and 8 times according to RFLP. This fixed non-random association of plasmid and chromosomal genotypes is consistent with a clonal population structure; it indicates limited exchange of plasmids under natural...... conditions. Seventeen isolates of 11 groups were highly effective and 2 isolates in one group almost ineffective....

  10. Environmental Factors Influencing Numbers of Rhizobium leguminosarum biovar trifolii and Its Bacteriophages in Two Field Soils

    OpenAIRE

    Lawson, Kerrie A.; Barnet, Yvonne M.; McGilchrist, Clyde A.

    1987-01-01

    Fluctuations in numbers of Rhizobium leguminosarum biovar trifolii and its bacteriophages in two fields with different soil types were followed during a 17-month period in 1981 and 1982. Mean levels of both phage and rhizobia varied significantly (P < 0.05) on different occasions, with rhizobial levels varying from 1.6 × 102 to 2.0 × 104 cell per g of soil and phage from 0 to 1.7 × 104 PFU/g of soil. Multivariate regression analysis showed rhizobial levels to be significantly and positively r...

  11. Competition Among Rhizobium leguminosarum Strains for Nodulation of Lentils (Lens esculenta)

    OpenAIRE

    May, Sheila N.; Bohlool, B. Ben

    1983-01-01

    Thirty-one cultures of Rhizobium leguminosarum were screened for effectiveness (C2H2 reduction) on lentils (Lens esculenta). Fluorescent antibodies prepared against three of the most effective strains (Hawaii 5-0, Nitragin 92A3, and Nitragin 128A12) exhibited a high degree of strain specificity; the antibodies reacted strongly with their homologous rhizobia in culture and with bacteroids in nodules. They did not cross-react with one another, and only weakly with 5 of the 47 other R. leguminos...

  12. Production and composition of extracellular polysaccharide synthesized by a Rhizobium isolate of Vigna mungo (L.) Hepper.

    Science.gov (United States)

    Mandal, Santi Mohan; Ray, Bimalendu; Dey, Satyahari; Pati, Bikas Ranjan

    2007-08-01

    An extracellular polysaccharide (EPS) was produced by a Rhizobium sp. isolated from the root nodules of Vigna mungo (L.) Hepper. Maximum EPS production (346 mg l(-1)) was when the yeast extract basal medium was supplemented with mannitol (1%), biotin (1.5 mg l(-1)) and asparagine (0.3%). Ribose (53%) and mannose (47%) were the principle monomers of the EPS. Chemical, chromatographic and spectroscopic analysis showed that this polymer, which has Man(4)Rib(1) as an oligomeric subunit, has an apparent molecular mass of 750 kDa.

  13. Underexpression of Ap from R-Plasmids in Fast-Growing Rhizobium Species

    Science.gov (United States)

    Sikka, Virendra K.; Kumar, Sushil

    1984-01-01

    The presence of the plasmid RP1 in the cells of Rhizobium leguminosarum strains Rld1, 300, and 248, R. phaseoli 1233, R. trifolii strains T1 and 6661, and R. meliloti 4013 was found to appreciably increase bacterial resistance toward kanamycin and tetracycline but not toward ampicillin. The presence of 16 other R-plasmids in R. leguminosarum was also found to either not increase or only marginally increase bacterial resistance toward ampicillin. It appears now that underexpression of the plasmid-specified ampicillin function is common to most fast- and slow-growing rhizobia. PMID:16346686

  14. 76 FR 38700 - United States, et al.

    Science.gov (United States)

    2011-07-01

    ... Antitrust Division United States, et al. v. American Express Company, et al.; Public Comments and Response...), the United States hereby publishes below its Response to public comments received on the proposed Final Judgment in United States, et al. v. American Express Company, et al., Civil Action No....

  15. ''Et sted''

    DEFF Research Database (Denmark)

    Dinesen, Cort Ross

    2009-01-01

    At arkitekturen er forbundet med og forankret til "et sted" - det at arkitekturen finder sted og at arkitekturen står et sted, - og at arkitekturen sætter en målelighed i det umålelige og skaber permanens er velkendt. Samtidig bevæger vi os fra "sted" til "sted" i værket eller mellem værker og vor...... hukommelse skaber en fornemmelse af en tidslig udstrækning i nuet; som noget der finder sted, som tilblivelse, som en åbning mod begivenheden. Det at vi bevæger os fra et her til et der udgør en forestilling om at rejse, vi ser og erfarer på nyt, vi tager ophold og tage steder i besiddelse. Vi tager ud i...... naturen, sætter os ved et træ, går langs vandet og bestiger højen, hvor vi danner et overblik og pejler et nyt mærke i landskabet - vi orientere os med alle vore sanser. På samme vis som i naturen, dvs. i kulturlandskabet, færdes vi i byens landskab....

  16. Studying Plant-Rhizobium Mutualism in the Biology Classroom: Connecting the Big Ideas in Biology through Inquiry

    Science.gov (United States)

    Suwa, Tomomi; Williamson, Brad

    2014-01-01

    We present a guided-inquiry biology lesson, using the plant-rhizobium symbiosis as a model system. This system provides a rich environment for developing connections between the big ideas in biology as outlined in the College Board's new AP Biology Curriculum. Students gain experience with the practice of scientific investigation, from…

  17. Population genomics of the symbiotic plasmids of sympatric nitrogen-fixing Rhizobium species associated with Phaseolus vulgaris.

    Science.gov (United States)

    Pérez Carrascal, Olga M; VanInsberghe, David; Juárez, Soledad; Polz, Martin F; Vinuesa, Pablo; González, Víctor

    2016-09-01

    Cultivated common beans are the primary protein source for millions of people around the world who subsist on low-input agriculture, enabled by the symbiotic N2 -fixation these legumes perform in association with rhizobia. Within a single agricultural plot, multiple Rhizobium species can nodulate bean roots, but it is unclear how genetically isolated these species remain in sympatry. To better understand this issue, we sequenced and compared the genomes of 33 strains isolated from the rhizosphere and root nodules of a particular bean variety grown in the same agricultural plot. We found that the Rhizobium species we observed coexist with low genetic recombination across their core genomes. Accessory plasmids thought to be necessary for the saprophytic lifestyle in soil show similar levels of genetic isolation, but with higher rates of recombination than the chromosomes. However, the symbiotic plasmids are extremely similar, with high rates of recombination and do not appear to have co-evolved with the chromosome or accessory plasmids. Therefore, while Rhizobium species are genetically isolated units within the microbial community, a common symbiotic plasmid allows all Rhizobium species to engage in symbiosis with the same host in a single agricultural plot. PMID:27312778

  18. Transfer and loss of naturally-occuring plasmids among isolates of Rhizobium leguminosarum bv. viciae in heavy metal contaminated soils

    NARCIS (Netherlands)

    Lakzian, A.; Murphy, P.J.; Giller, K.E.

    2007-01-01

    Plasmid transfer among isolates of Rhizobium leguminosarum bv. viciae in heavy metal contaminated soils from a long-term experiment in Braunschweig, Germany, was investigated under laboratory conditions. Three replicate samples each of four sterilized soils with total Zn contents of 54, 104, 208 and

  19. Rhizobium leguminosarum bv. viciae populations in soils with increasing heavy metal contamination: abundance, plasmid profiles, diversity and metal tolerance

    NARCIS (Netherlands)

    Lakzian, A.; Murphy, P.; Turner, A.; Beynon, J.L.; Giller, K.E.

    2002-01-01

    Populations of Rhizobium leguminosarum bv. viciae were investigated from plots of a long-term sewage sludge experiment in Braunschweig, Germany, which represented a gradient of increasing metal contamination. The number of R. leguminosarum bv. viciae decreased from 105 cells g¿1 soil in uncontaminat

  20. Production of Proteasome Inhibitor Syringolin A by the Endophyte Rhizobium sp. Strain AP16

    Science.gov (United States)

    Bigler, Laurent; Dudler, Robert

    2014-01-01

    Syringolin A, the product of a mixed nonribosomal peptide synthetase/polyketide synthase encoded by the syl gene cluster, is a virulence factor secreted by certain Pseudomonas syringae strains. Together with the glidobactins produced by a number of beta- and gammaproteobacterial human and animal pathogens, it belongs to the syrbactins, a structurally novel class of proteasome inhibitors. In plants, proteasome inhibition by syringolin A-producing P. syringae strains leads to the suppression of host defense pathways requiring proteasome activity, such as the ones mediated by salicylic acid and jasmonic acid. Here we report the discovery of a syl-like gene cluster with some unusual features in the alphaproteobacterial endophyte Rhizobium sp. strain AP16 that encodes a putative syringolin A-like synthetase whose components share 55% to 65% sequence identity (72% to 79% similarity) at the amino acid level. As revealed by average nucleotide identity (ANI) calculations, this strain likely belongs to the same species as biocontrol strain R. rhizogenes K84 (formely known as Agrobacterium radiobacter K84), which, however, carries a nonfunctional deletion remnant of the syl-like gene cluster. Here we present a functional analysis of the syl-like gene cluster of Rhizobium sp. strain AP16 and demonstrate that this endophyte synthesizes syringolin A and some related minor variants, suggesting that proteasome inhibition by syrbactin production can be important not only for pathogens but also for endophytic bacteria in the interaction with their hosts. PMID:24727275

  1. Performance of fenugreek bioinoculated with Rhizobium meliloti strains under semi-arid condition.

    Science.gov (United States)

    Singh, N K; Patel, D B

    2016-01-01

    Rhizobium meliloti strains were isolated from the fields of S.D. Agricultural University (Gujarat, India) and were maintained in the Congo Red Yeast Extract Mannitol Agar medium. These strains were tested for their effectiveness for fenugreek crop grown under semi-arid condition. Among the six Rhizobium strains, FRS-7 strain showed best plant growth parameters like shoot length, shoot dry weight, shoot total nitrogen, root length, root dry weight, root total nitrogen, seed yield, 1000 grain weight, number of root nodules, and nodules fresh and dry weight. The performance of this strain was better as compared to 20 kgN ha(-1) treatment through urea and was even far better over control plot. Seed yields obtained with FRS-7 during two years were 10.14 and 9.66 q ha(-1); which was about 36.8% and 45.9% high over control. This strain resulted in saving of about 20 kgN ha(-1) accompanied with better crop yield and soil health. Results of the present experiments can be utilized in integrated nutrient management for cultivation of fenugreek in semi-arid areas to provide sustainability to agricultural productivity in such regions. PMID:26930857

  2. Variability of isolated colonies in bean nodulating Rhizobium strains before and after exposure to high temperature

    Directory of Open Access Journals (Sweden)

    Raposeiras Rui

    2002-01-01

    Full Text Available Irregular response to bean plants to Rhizobium inoculation has been attributed to among other factors, low competitive ability, low N2 fixation efficiency and genetic instability of the symbiont. This genetic instability caused by high rates of genomic rearrangements and/or plasmid deletions can be accentuated by high temperatures. This fact may limit the utilization of these strains as inoculants, especially in tropical soils. In this study, the variability of isolated colonies derived from effective R. leguminosarum bv. phaseoli (SLP1.3 and BR 10.026 and R tropici (SLA2.2 and BR322 strains was evaluated before and after exposure to high temperatures (four consecutive thermal shocks at 45masculineC. This evaluation involved plant dry matter analysis of inoculated plants and genotypic (plasmid profile and genomic patterns via RAPD analysis of the Rhizobium strains. The results evidenced that high temperature improve the natural performance variability especially between isolated colonies from R. leguminosarum bv. phaseoli strains. The plasmid profile of isolated colonies from R. tropici strains were identical regardless of temperature treatment whereas isolated colonies from R. leguminosarum bv. phaseoli alterations were detected especially after the thermal treatment. The genomic patterns generated by AP-PCR showed more alterations and genetic variation in isolated colonies from R. leguminosarum bv. phaseoli strains indicating that R. tropici strains are more stable and lower affected by high temperature.

  3. Mimosine Content of Leucaena leucocephala and the Sensitivity of Rhizobium to Mimosine.

    Science.gov (United States)

    Mathews, A; Vittal Rai, P

    1985-01-01

    Mimosine was qualitatively and quantitatively estimated from the different parts of Leucaena leucocephala strains K-8 and K-67. The amount of mimosine as percentage of dry weight in the two strains were, respectively, as follows: seeds, 6.82, 9.98; old leaves, 1.5, 1.33; young leaves, 2.85, 3.33; main root, 1.13, 1.02; lateral roots, 1.46, 1.17; stems, 0.73, 0.33. Rhizobium isolates from L. leucocephala (UAS-Ll-8, UAS-Ll-67), Mimosa pudica (UAS-Mp) and Prosopis juliflora (UAS-Pj) formed an effective (nod(+) fix(+)) association with Leucaena, while an isolate from Crotalaria sp. (UAS-Cr) proved to be ineffective (nod(+) fix(-)). All isolates performed better than reference strain TAL-82. Strain UAS-Pj gave optimum nodulation and plant growth characteristics. The effect of mimosine on the vegetative Rhizobium cultures was studied by the lawn method of growing rhizobia, using 2.5 to 15 mg·ml(-1) mimosine. All the effective strains were stimulated by mimosine up to 7.5 mg·ml(-1)strains. UAS-Ll-8 and UAS-Ll-67 were stimulated up to 15 mg·ml(-1) of mimosine. The ineffective strain UAS-Cr was inhibited at all the concentrations of mimosine tried.

  4. Synergism Among VA Mycorrhiza, Phosphate Solubilizing Bacteria and Rhizobium for Symbiosis with Blackgram (Vigna mungo L.) UnderField Conditions

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A field experiment was conducted at the G.B. Pant University Research Station, Ujhani (U.P.) in rainy (Kharif) season of the year 1994-1995 to study the effect of Rhizobium, VAM (vesicular arbuscular mycorrhiza) and PSB (phosphate solubilizing bacteria) inoculation, with and without P, on blackgram (Vigna mungo L.) seed yield. Phosphorus application in soil with medium P content (5.4 mg kg1) increased nodulation, grain yield, N and P in plant and grain over no phosphorus control. Forty kilograms of P2O5 each hactare recorded an increase of 20.6 % in nodule dry weight, significant increases of 0.35 g kg-1 in N concentration and 1.28 g kg-1 in P concentration of plant over 20 kg P2O5 ha-1. Similar significant increases of 0.59 g kg-1 in grain yield and 0.54 and 0.23 g kg-1 in N and P concentrations of the grain, respectively, over 20 kg P2O5 ha-1 were also obtained with higher dose. Inoculation of Rhizobium + VAM + PSB at all the stages of plant growth recorded maximum increases in all the parameters studied. Dual inoculation of Rhizobium with either VAM or PSB was generally significant in the effect and better than that of VAM + PSB, however, P accumulation in plant and grain was more with VAM + PSB. Among single inoculations, Rhizobium gave highest and 21.0 % more nodule number, 34.7 % more nodule dry mass, 0.73 g kg-1 more N in grain and 4.2 % higher grain yield over PSB. PSB, however, registered significant increases in P concentration in plant and grain over VAM and Rhizobium.

  5. Enhanced Survival and Nodule Occupancy of Pigeon pea Nodulating Rhizobium sp. ST1 expressing fegA Gene of Bradyrhizobium japonicum 61A152

    Directory of Open Access Journals (Sweden)

    G. Archana

    2009-01-01

    Full Text Available Problem statement: Rhizobial isolates belonging to genera (Rhizobium sp. and Mesorhizobium sp. in our laboratory produced only catecholate type of siderophores. Although FhuA and FegA (ferrichrome receptors homologs were found to be present in the sequenced genomes of few rhizobia (e.g., 1 in R. etli and 2 in Mesorhizobium sp. BNC1, laboratory isolates of the corresponding genera failed to utilize ferrichrome, a siderophore which is present in nanomolar concentrations in the soil. This inability was considered as a negative fitness factor with respect to rhizospheric colonization by these rhizobia. Approach: The 2.4 kb fegA gene (encoding ferrichrome receptor was amplified along with its native promoter from Bradyrhizobium japonicum 61A152 and cloned in a broad host range plasmid vector pUCPM18. The plasmid construct pFJ was transferred by conjugation into Rhizobium sp. ST1 to give transconjugant ST1pFJ12. The consequence of FegA expression on the transconjugant was tested under lab and soil conditions, using physiological experiments. Results: Ability of the transconjugant ST1pFJ12 to utilize ferrichrome and expression of a 79 kD protein band on the outer membrane of the transconjugant confirmed FegA expression. Transconjugant ST1pFJ12 exhibited increased growth rate as compared to the parent strain ST1, in minimal media containing ferrichrome as the sole iron source, confirming the positive effect of FegA expression. Inoculation of pigeon pea seedlings with transconjugant ST1pFJ12 led to a marked increase in plant growth parameters as compared to plants inoculated with the parent strain ST1, the effect being more pronounced when Ustilago maydis, a ferrichrome producer was co-inoculated in the systems. Nodule occupancy on pigeon pea plant when inoculated with the transconjugant ST1pFJ12 alone was 57% which increased to 66% when co-inoculated with U. maydis as compared with 37 and 30

  6. Final Environmental Statement related to the operation of Wolf Creek Generating Station, Unit No. 1. Docket No. STN 50-482, Kansas Gas and Electric Company, et al

    International Nuclear Information System (INIS)

    This final environmental statement contains the second assessment of the environmental impact associated with operation of Wolf Creek Generating Station Unit 1 pursuant to the National Environmental Policy Act of 1969 (NEPA) and 10 CFR Part 51, as amended, of the NRC's regulations. This statement examines: the affected environment, environmental consequences and mitigating actions, and environmental and economic benefits and costs. Land use and terrestrial- and aquatic-ecological impacts will be small. Air-quality impacts will also be small. However, steam fog from the station's cooling lake has the potential for reducing visibility over nearby roads and bridges. A fog-monitoring program for roads and bridges near the lake has been recommended. Impacts to historic and prehistoric sites will be negligible. Chemical discharges to the Neosho River are expected to have no appreciable impacts on water quality under normal conditions and will be required to meet conditions of the station's NPDES permit. The effects of routine operations, energy transmission, and periodic maintenance of rights-of-way and transmission line facilities should not jeopardize any populations of endangered or threatened species. No significant impacts are anticipated from normal operational releases of radioactivity. The risk associated with accidental radiation exposure is very low. The net socioeconomic effects of the project will be beneficial. The action called for is the issuance of an operating license for the Wolf Creek Generating Station Unit 1

  7. Et hjem

    DEFF Research Database (Denmark)

    Bruun, Maja Hojer

    2014-01-01

    Når folk sætter deres hjem til salg - især når der hersker usikkerhed om værdier og priser som under de seneste års boligkrise - får det os til at tænke over, hvad vilkårene er for at stifte, skabe og genskabe hjem i et moderne markedsbaseret samfund: Den integrerede helhed af personer, ting og...... sted, som hjemmet udgør i hverdagslivet, opløses, når et hjem sættes til salg og gøres til en vare på et boligmarked. Til hjemmets potentiale som vare knytter der sig drømme og forventninger til fremtiden - og i krisetider utryghed og uhygge....

  8. Mission related to regulation and development of the photovoltaic sector in France. Final report; Mission relative a la regulation et au developpement de la filiere photovoltaique en France. Rapport final

    Energy Technology Data Exchange (ETDEWEB)

    Charpin, J.M; Sine, A.; Helleisen, Ph.; Tlili, C. [Inspection Generale des Finances - IGF, 75 - Paris (France); Trink, C.; Stoffaes, Ch. [Conseil General de l' Industrie de l' Energie et des Technologies - CGIET, 75 - Paris (France)

    2010-07-01

    This report first gives an overview of the development of the photovoltaic stock in France (high potential with a middle term development constrained by high costs, consequences of a very strong increase of demands at the end of 2009, perspective of development at a rather high rate). Then, it outlines and discusses the financial consequences of such a high rate development, and the inadequacy between industrial objectives and development rate. It presents a development plan for this sector, stresses the need to implement new purchase obligation measures before the summer 2010, and to define a global action plan to be implemented at the beginning of 2011 for the development of photovoltaic electricity. Elements of international comparison are finally proposed

  9. Et evalueringseksperiment

    DEFF Research Database (Denmark)

    Christensen, Torben Spanget

    2008-01-01

    Artiklen præsenterer et evalueringseksperiment foretaget i 8 klasserum. Der er tale om mundtlige formative evalueringer som har fokus på elevernes faglige viden. Artiklen præsenterer en model for fagligt evaluerende lærer-elevsamtaler (FE-samtaler) som er udviklet i eksperimentet og resultaterne af...

  10. Effectiveness of halo-tolerant, auxin producing Pseudomonas and Rhizobium strains to improve osmotic stress tolerance in mung bean (Vigna radiata L.

    Directory of Open Access Journals (Sweden)

    Maqshoof Ahmad

    2013-12-01

    Full Text Available Halo-tolerant, auxin producing bacteria could be used to induce salt tolerance in plants. A number of Rhizobium and auxin producing rhizobacterial strains were assessed for their ability to tolerate salt stress by conducting osmoadaptation assay. The selected strains were further screened for their ability to induce osmotic stress tolerance in mung bean seedlings under salt-stressed axenic conditions in growth pouch/jar trials. Three most effective strains of Rhizobium and Pseudomonas containing ACC-deaminase were evaluated in combination, for their ability to induce osmotic stress tolerance in mung bean at original, 4, and 6 dS m-1 under axenic conditions. Results showed that sole inoculation of Rhizobium and Pseudomonas strains improved the total dry matter up to 1.4, and 1.9 fold, respectively, while the increase in salt tolerance index was improved up to 1.3 and 2.0 fold by the Rhizobium and Pseudomonas strains, respectively. However, up to 2.2 fold increase in total dry matter and salt tolerance index was observed due to combined inoculation of Rhizobium and Pseudomonas strains. So, combined application of Rhizobium and Pseudomonas strains could be explored as an effective strategy to induce osmotic stress tolerance in mung bean.

  11. Functional characterization of aroA from Rhizobium leguminosarum with significant glyphosate tolerance in transgenic Arabidopsis.

    Science.gov (United States)

    Han, Jing; Tian, Yong-Sheng; Xu, Jing; Wang, Li-Juan; Wang, Bo; Peng, Ri-He; Yao, Quan-Hong

    2014-09-01

    Glyphosate is the active component of the top-selling herbicide, the phytotoxicity of which is due to its inhibition of the shikimic acid pathway. 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) is a key enzyme in the shikimic acid pathway. Glyphosate tolerance in plants can be achieved by the expression of a glyphosate-insensitive aroA gene (EPSPS). In this study, we used a PCR-based two-step DNA synthesis method to synthesize a new aroA gene (aroAR. leguminosarum) from Rhizobium leguminosarum. In vitro glyphosate sensitivity assays showed that aroAR. leguminosarum is glyphosate tolerant. The new gene was then expressed in E. coli and key kinetic values of the purified enzyme were determined. Furthermore, we transformed the aroA gene into Arabidopsis thaliana by the floral dip method. Transgenic Arabidopsis with the aroAR. leguminosarum gene was obtained to prove its potential use in developing glyphosate-resistant crops.

  12. Inhibition of nodule development by multicopy promoters of Rhizobium meliloti nif/fix genes

    Institute of Scientific and Technical Information of China (English)

    吴桐; 朱家璧; 俞冠翘; 沈善炯

    1995-01-01

    Using luc gene as a reporter to study the activation of Rhizobium meliloti nif/fix genes in thedevelopment of symbiosis,the authors observed that nodule development and nitrogen fixation were inhibitedby both multicopy promoters of nifHDK and fixABCX.The phenotype of R.meliloti containing multicopynif/fix promoters appeared exactly like that of nifA mutant.Using lacZ as a reporter,the authors got the same re-sults.By contrast,the rhizobia containing low-copy promoters of nif/fix genes were normal fornodule development and nitrogen fixation.These results substantiate the evidence that the product of nifAgene not only acts as a transcriptional activator of nif/fix genes,but also plays an important role in thedevelopment of root nodules.

  13. Environmental Factors Influencing Numbers of Rhizobium leguminosarum biovar trifolii and Its Bacteriophages in Two Field Soils.

    Science.gov (United States)

    Lawson, K A; Barnet, Y M; McGilchrist, C A

    1987-05-01

    Fluctuations in numbers of Rhizobium leguminosarum biovar trifolii and its bacteriophages in two fields with different soil types were followed during a 17-month period in 1981 and 1982. Mean levels of both phage and rhizobia varied significantly (P soil and phage from 0 to 1.7 x 10 PFU/g of soil. Multivariate regression analysis showed rhizobial levels to be significantly and positively related to vegetation height and solar radiation, but not to mean temperature, precipitation, soil matric potential, or soil type. Rhizobiophage concentrations were significantly and positively related to soil matric potential and vegetation height. They were reduced in the silty clay loam soil, although the presence of 34% clay did not prevent phage multiplication and the occurrence of high phage levels. PMID:16347339

  14. Regulation of symbiotic nitrogen fixation in root nodules of alfalfa (Medicago sativa) infected with Rhizobium meliloti.

    Science.gov (United States)

    Kamberger, W

    1977-10-24

    Symbiotic nitrogen fixation of Rhizobium meliloti bacteroids in Medicago sativa root nodules was suppressed by several inorganic nitrogen sources. Amino acids like glutamine, glutamic acid and aspartic acid, which can serve as sole nitrogen sources for the unnodulated plant did not influence nitrogenase activity of effective nodules, even at high concetrations. Ammonia and nitrate suppressed symbiotic nitrogen fixation in vivo only at concentrations much higher than those needed for suppression of nitrogenase activity in free living nitrogen fixing bacteria. The kinetics of suppression were slow compared with that of free living nitrogen fixing bacteria. On the other hand, nitrite, which acts as a direct inhibitor of nitrogenase, suppressed very quickly and at low concentrations. Glutamic acid and glutamine enhanced the effect of ammonia dramatically, while the suppression by nitrate was enhanced only slightly.

  15. Mekanisme Penambatan Nitrogen Udara oleh Bakteri Rhizobium Menginspirasi Perkembangan Teknologi Pemupukan Organik yang Ramah Lingkungan

    Directory of Open Access Journals (Sweden)

    GEDE MENAKA ADNYANA

    2014-01-01

    Full Text Available Interaction of microbes with human living in the nature are able to seen from their capabilitiesimproving the environmental by composting waste of agriculture to become organic fertilizer. In thecontrary, the epidemic and infectious phatogens are also its contribution. In agriculture sector, the livingof microbes has been explored to keep the soil fertile, and to improve growth and plant production.The symbiosis of Rhizobium bacteria with the specific host plant from legumes is able to run because ofboth side giving a signal, where host plant give an organic molecoles called inducer. From the bacteriaside, also give an organic materials called nod factor. The step of nitrogen fixation mechanism can bedescribed from infection of root legume as the first step, followed by the nitrogen fixation by thebacteroid and synthesis of the nitrogen organic by host plant as the last step. These natural processesinspire finding and development of the ecologically organic fertilizer technology.

  16. Isolation and characterization of Rhizobium meliloti mutants affected in exopolysaccharide production.

    Science.gov (United States)

    Rodríguez-Navarro, D N; Palomares, A J; Casadesús, J

    1991-06-01

    Rhizobium meliloti mutants affected in the production of exopolysaccharide (EPS) were isolated after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The mutants were classified into three phenotypic classes: (I) Exo-, rough mutants lacking exopolysaccharide; (II) Exos (for "small") which form tiny, compact colonies and synthesize reduced amounts of EPS; and (III) Exoc (for "constitutive"), hypermucoid mutants which overproduce EPS. Hypermucoid strains showed increased resistance to desiccation. All the mutants were able to nodulate, although a significant decrease in infectivity degree and/or competitiveness was found in rough and compact strains. Two mutants proved to be deficient in nitrogen fixation. Complementation analysis with cloned R. meliloti exo genes could not be applied to the study of these Fix- mutants because introduction of plasmids derived from cosmid vector pLAFR1 caused loss of nodulating ability. However, complementation of calcofluor staining and EPS production was observed. Complementation with certain exo genes also caused a marked increase in motility.

  17. Effect of Rhizobium inoculation of seeds and foliar fertilization on productivity of Pisum sativum L.

    Directory of Open Access Journals (Sweden)

    Tadeusz Zając

    2013-07-01

    Full Text Available Pea (Pisum sativum L. is the second most important grain legume crop in the world which has a wide array of uses for human food and fodder. One of the major factors that determines the use of field pea is the yield potential of cultivars. Presently, pre-sowing inoculation of pea seeds and foliar application of microelement fertilizers are prospective solutions and may be reasonable agrotechnical options. This research was undertaken because of the potentially high productivity of the 'afila' morphotype in good wheat complex soils. The aim of the study was to determine the effect of vaccination with Rhizobium and foliar micronutrient fertilization on yield of the afila pea variety. The research was based on a two-year (2009–2010 controlled field experiment, conducted in four replicates and carried out on the experimental field of the Bayer company located in Modzurów, Silesian region. experimental field soil was Umbrisol – slightly degraded chernozem, formed from loess. Nitragina inoculant, as a source of symbiotic bacteria, was applied before sowing seeds. Green area index (GAI of the canopy, photosynthetically active radiation (PAR, and normalized difference vegetation index (NDVI were determined at characteristic growth stages. The presented results of this study on symbiotic nitrogen fixation by leguminous plants show that the combined application of Nitragina and Photrel was the best combination for productivity. Remote measurements of the pea canopy indexes indicated the formation of the optimum leaf area which effectively used photosynthetically active radiation. The use of Nitragina as a donor of effective Rhizobium for pea plants resulted in slightly higher GAI values and the optimization of PAR and NDVI. It is not recommended to use foliar fertilizers or Nitragina separately due to the slowing of pea productivity.

  18. Inoculação com Rhizobium e aplicação de nitrogênio em amendoim Comparison among Rhizobium strains inoculations and nitrogen applications on peanut, in field conditions

    OpenAIRE

    Antonio Roberto Giardini; Eli Sidney Lopes; Angelo Savy Filho; André Martin Louis Neptune

    1985-01-01

    Existe, nas nossas condições, uma população autóctone de Rhizobium capaz de nodular o amendoim (Arachis hypogaea L.), mas pouco se sabe da contribuição do nitrogênio fixado para esta planta. Foram conduzidos dois ensaios no campo, em solo de baixa fertilidade, um no período "da seca" e outro no "das águas", comparando o crescimento e a produção de plantas de amendoim inoculado com Rhizobium selecionado, com o de plantas noduladas pela população autóctone, adubadas ou não com nitrogênio. A nod...

  19. Stimulation of indoleacetic acid production in a Rhizobium isolate of Vigna mungo by root nodule phenolic acids.

    Science.gov (United States)

    Mandal, Santi M; Mandal, Santi; Mandal, Mahitosh; Das, Amit K; Das, Amit; Pati, Bikas R; Pati, Bikas; Ghosh, Ananta K; Ghosh, Ananta

    2009-04-01

    The influence of endogenous root nodules phenolic acids on indoleacetic acid (IAA) production by its symbiont (Rhizobium) was examined. The root nodules contain higher amount of IAA and phenolic acids than non-nodulated roots. Presence of IAA metabolizing enzymes, IAA oxidase, peroxidase, and polyphenol oxidase indicate the metabolism of IAA in the nodules and roots. Three most abundant endogenous root nodule phenolic acids (protocatechuic acid, 4-hydroxybenzaldehyde and p-coumaric acid) have been identified and their effects on IAA production by the symbiont have been studied in L-tryptophan supplemented yeast extract basal medium. Protocatechuic acid (1.5 microg ml(-1)) showed maximum stimulation (2.15-fold over control) of IAA production in rhizobial culture. These results indicate that the phenolic acids present in the nodule might serve as a stimulator for IAA production by the symbiont (Rhizobium).

  20. Mutation of the Sensor Kinase chvG in Rhizobium leguminosarum Negatively Impacts Cellular Metabolism, Outer Membrane Stability, and Symbiosis

    OpenAIRE

    Vanderlinde, Elizabeth M.; Yost, Christopher K.

    2012-01-01

    Two-component signal transduction systems (TCS) are a main strategy used by bacteria to sense and adapt to changes in their environment. In the legume symbiont Rhizobium leguminosarum biovar viciae VF39, mutation of chvG, a histidine kinase, caused a number of pleiotropic phenotypes. ChvG mutants are unable to grow on proline, glutamate, histidine, or arginine as the sole carbon source. The chvG mutant secreted smaller amounts of acidic and neutral surface polysaccharides and accumulated abno...

  1. Compatibility of Rhizobial Genotypes within Natural Populations of Rhizobium leguminosarum Biovar viciae for Nodulation of Host Legumes

    OpenAIRE

    Laguerre, Gisèle; Louvrier, Philippe; Allard, Marie-Reine; AMARGER, Noëlle

    2003-01-01

    Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean (Vicia faba) and pea (Pisum sativum) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches (Vicia sativa) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia were characterized by repetitive extragenomic palindromic-PCR fingerprinting and/or PCR-restriction ...

  2. Two rhizobacterial strains, individually and in interactions with Rhizobium sp., enhance fusarial wilt control, growth, and yield in pigeon pea.

    Science.gov (United States)

    Dutta, Swarnalee; Morang, Pranjal; Kumar S, Nishanth; Dileep Kumar, B S

    2014-09-01

    A Pseudomonas aeruginosa strain, RRLJ 04, and a Bacillus cereus strain, BS 03, were tested both individually and in combination with a Rhizobium strain, RH 2, for their ability to enhance plant growth and nodulation in pigeon pea (Cajanus cajan L.) under gnotobiotic, greenhouse and field conditions. Both of the rhizobacterial strains exhibited a positive effect on growth in terms of shoot height, root length, fresh and dry weight, nodulation and yield over the non-treated control. Co-inoculation of seeds with these strains and Rhizobium RH 2 also reduced the number of wilted plants, when grown in soil infested with Fusarium udum. Gnotobiotic studies confirmed that the suppression of wilt disease was due to the presence of the respective PGPR strains. Seed bacterization with drug-marked mutants of RRLJ 04 and BS 03 confirmed their ability to colonize and multiply along the roots. The results suggest that co-inoculation of these strains with Rhizobium strain RH 2 can be further exploited for enhanced growth, nodulation and yield in addition to control of fusarial wilt in pigeon pea. PMID:25224506

  3. Effcet of Rhizobium Inoculation on Growth and Nodulation of Albizzia procera,Albizzia lebbeck and Leucaena leucocephala

    Institute of Scientific and Technical Information of China (English)

    U.K.ARYAL; M.A.U.MRIDHA; 等

    1999-01-01

    Effects of inoculation of Rhizobium suspension on nodulation and plant growth were examined with Albizzia procera,Albizzia lebbeck and Leucaena leucocephala seedlings grown on sterilized and non-sterilized soil media.Inoculation resulted in nodule number increases of 28.6,29.02and 23.9 times in sterilized soil and 3.4,3.6and 3.27 times in non-sterilized soil for A.procera,A.lebbeck and L.leucocephala seedlings respectively.Total dry mass increased by 127.6%,66.7%and 60.7% in sterilized soil and 100%,95.5%and 52.65% in non-sterilized soil for these three legume trees,respectively,after a period of two months.Significantly high inoculation responses of oot length,root diameter,collar diameter,shoot length,and dry mass of root,shoot,leaves and nodules were also observed in both steilized and non-sterilized soil media as compared to respective control treatments,The response to inoculation was strong in sterilized and modest in non-sterilized soils.The significantly higher response to Rhizobium inoculation over control in all the species tested suggested that application of Rhizobium greatly enhanced plant growth ,nodulation,biomass production and nitrogen-fixing activity of the nodules.

  4. Symbiont effect of Rhizobium bacteria and vesicular arbuscular mycorrhizal fungi on Pisum sativum in recultivated mine spoils

    Energy Technology Data Exchange (ETDEWEB)

    Biro, B.; Voros, I.; Kovespechy, K.; Szegi, J. (Hungarian Academy of Sciences, Budapest (Hungary). Soil Science and Agricultural Chemistry Research Institute)

    The frequency (F%) of spontaneous endomycorrhizal fungal infection (AMF) and the root modulation of Pisum sativum L, was studied after 8 and 15 years of recultivation in 4 soils (andesitic tuff yellow sand, yellow clay, and grey clay) disturbed by mining activities. The effects of Rhizobium inoculation and the interaction of both microsymbionts with plant production were also examined along with humus content and the humus stability coefficient, in the following variations: control, NPK fertilizer, NPK+lignite, NPK+straw and sewage sludge. Dump spoils originating from deep geological layers were poor in organic materials. After 12 years of recultivation, the humus content increased significantly. No such increase was noted in grey clay and the natural, brown forest soil used as an undisturbed (control) sample. The degradation of soils by mining brings about a decrease in the rhizobial and mycorrhizal population, so the number of spontaneous Rhizobium nodules is relatively low and does not influence the yield of peas. Inoculation with a selected effective strain of Rhizobium leguminosarum bv. viceae, however, enhanced dry matter production in these microbiologically degraded soils. Although the frequency of AMF infection was also higher after rhizobial inoculation, no positive correlation was found between dry matter production of peas and the F% of AMF. Spoils treated with lignite, straw, and sewage sludge had an especially high level of spontaneous mycorrhizal populations, indicating that organic materials had a favorable effect on the recultivation processes and fertility of mine spoils.

  5. Selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L. on the sand sterile medium in greenhouse

    Directory of Open Access Journals (Sweden)

    SRI PURWANINGSIH

    2005-07-01

    Full Text Available An experiment on the selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L. on the sand sterile medium in green house. The aim of the experiment the selection and potency of the Rhizobium strain to increase the growth of soybean. The experiment was carried out in green house condition in Microbiology Division, Research Center for Biology-LIPI with sterile sand medium. The research design was Completely Randomized Design with three replications for each treatment. The Rhizobium strains used were 1 W (isolated from bean, Vigna radiata, 2 W (isolated from soybean, 3 W (isolated from bean, 4 W (isolated from soybean, 5 W (isolated from soybean, 6 W (isolated from peanut, Arachis hypogaea, 7 W (isolated from peanut, 8 W (isolated from peanut, the controls were uninoculated with Rhizobium strain and without urea fertilizer (K1, uninoculated and with urea fertilizer equal 100 kg/ha (K2. The plants were harvested after 50 days, the variable of investigation were the dry weight of canopy, roots, nodules root, total plants, number of nodules and ‘symbiotic capacity”. The results showed that all of experiment plant which be inoculated with Rhizobium able to form nodule. Strain of 2 W (isolated from soybean has given the best effects on the growth of soybean.

  6. Problemes methodologiques poses par l'extraction et la recuperation des bacteries telluriques pour leur quantification par immunofluorescence

    OpenAIRE

    Crozat, Y.; Cleyet-Marel, Jean Claude

    1984-01-01

    Afin d’améliorer la valeur quantitative de la technique de dénombrement des bactéries telluriques (Rhizobium japonicum) par immunofluorescence, on compare différents modes d’extraction et de récupération. L’utilisation de membranes en polycarbonates (Nucléopores) supprime les 40 p. 100 de pertes occasionnées par les membranes cellulosiques (Millipores) classiquement employées. Parmi les différents floculants testés, Ca(OH)2/MgCO3(2/5) permet d’obtenir les meilleurs résultats. Cependant po...

  7. Increased effectiveness of competitive rhizobium strains upon inoculation of Cajanus cajan

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez, B.S.; Poth, M.; Focht, D.D.

    1987-09-01

    A field study was conducted in lysimeters containing /sup 15/N-enriched soil to determine the effects of four competitive rhizobium strains upon yield parameters of pigeon peas (Cajanus cajan). The greatest differences observed were in seed yields; strain P132 effected the highest seed yield (121 +/- 20 g per plant), and the control strain (indigenous rhizobia) effected the lowest yield (43.9 +/- 8 g per plant). With the exception of seeds and pods, the dry matter weights were not different. Although there appeared to be no effect by inoculum strains on the fractional content of N derived from biological nitrogen fixation when the total plant biomass was considered, strains P132 and 401 partitioned more of the N derived from fixation into seeds and leaves than did the other strains. Because the seeds comprised the major portion of plant N, more total N and more N derived from biological nitrogen fixation (about half of total N) were found in plants inoculated with P132, whereas the smallest amount was found in the uninoculated controls. P132 was also the best competitor with respect to indigenous rhizobia and accounted for all of the nodules found on the plants in which it was inoculated.

  8. Transformation of pWWO in Rhizobium leguminosarum DPT to Engineer Toluene Degrading Ability for Rhizoremediation.

    Science.gov (United States)

    Goel, Garima; Pandey, Piyush; Sood, Anchal; Bisht, Sandeep; Maheshwari, D K; Sharma, G D

    2012-06-01

    Rhizoremediation of organic xenobiotics is based on interactions between plants and their associated micro-organisms. The present work was designed to engineer a bacterial system having toluene degradation ability along with plant growth promoting characteristics for effective rhizoremediation. pWWO harboring the genes responsible for toluene breakdown was isolated from Pseudomonas putida MTCC 979 and successfully transformed in Rhizobium DPT. This resulted in a bacterial strain (DPT(T)) which had the ability to degrade toluene as well as enhance growth of host plant. The frequency of transformation was recorded 5.7 × 10(-6). DPT produced IAA, siderophore, chitinase, HCN, ACC deaminase, solubilized inorganic phosphate, fixed atmospheric nitrogen and inhibited the growth of Fusarium oxysporum and Macrophomina phaseolina in vitro. During pot assay, 50 ppm toluene in soil was found to inhibit the germination of Cajanus cajan seeds. However when the seeds bacterized with toluene degrading P. putida or R. leguminosarum DPT were sown in pots, again no germination was observed. Non-bacterized as well as bacterized seeds germinated successfully in toluene free soil as control. The results forced for an alternative mode of application of bacteria for rhizoremediation purpose. Hence bacterial suspension was mixed with soil having 50 ppm of toluene. Germination index in DPT treated soil was 100% while in P. putida it was 50%. Untreated soil with toluene restricted the seeds to germinate. PMID:23729882

  9. Rhizobium Strain Effects on Yield and Bleeding Sap Amino Compounds in Pisum sativum

    DEFF Research Database (Denmark)

    Rosendahl, Lis

    1984-01-01

    Bleeding sap composition, dry matter production and N distribution in pea (P. sativum L. cv. Bodil) grown with and without nitrate and nodulated with either R. leguminosarum strain 128c53 or strain 1044 were compared. Nitrate increased the total dry matter production of both symbioses, but decrea......Bleeding sap composition, dry matter production and N distribution in pea (P. sativum L. cv. Bodil) grown with and without nitrate and nodulated with either R. leguminosarum strain 128c53 or strain 1044 were compared. Nitrate increased the total dry matter production of both symbioses...... relative to the total N-accumulation was greater with strain 128c53 due to a higher production of nodule tissue. The root bleeding sap of the symbiosis with the greater yield (strain 1044) contained high levels of asparagine and aspartic acid. In the 128c53 symbiosis, glutamine plus homoserine accounted...... for a higher percentage of the organic solutes transporting newly assimilated N from the root system than in the association with 1044. The Rhizobium strain effect on amino compound composition of the bleeding sap may indicate an influence of the bacteroids on either the N-assimilatory enzyme system...

  10. Study of the basis for the competitiveness of Rhizobium japonicum in the nodulation of soybean

    Energy Technology Data Exchange (ETDEWEB)

    Bauer, W.

    1990-06-01

    The overall goal of our studies was to identify cellular and molecular characteristics of rhizobia that are important to root colonization and symbiotic infection in competition with indigenous soil microbes. Rhizobia were found to respond to specific host flavonoids as chemoattractants as well as nodulation gene inducers. Motility and chemotaxis contributed significantly to the efficiency of symbiotic interactions, but not to colonization of root tips, where bacterial dispersal depended primarily on passive movement by root cell elongation. Co-inoculation of Rhizobium nod gene mutants with each other and with the wild-type showed that the mutants could help the wild-type, but not each other, to initiate nodules. Mutants with defective host specificity genes were the only ones capable of helping the wild-type in an additive or synergistic manner. Alfalfa was shown to have rapid, systemic feedback regulation suppressing nodule formation in younger parts of the root system in response to initiation of the first nodules. Root mucilage and exudate components were found to affect attachment of rhizobia to root surfaces. Methods for optimal surface sterilization of legume seeds were examined. 5 refs.

  11. Production of extracellular biopolymers and identification of intracellular proteins and Rhizobium tropici.

    Science.gov (United States)

    Oliveira, José; Figueiredo, Marcia; Silva, Marcia; Malta, Marília; Vendruscolo, Claire; Almeida, Hélio

    2012-12-01

    The objective of this study was to identify species of rhizobia (from the IPA 403 and IPA 49 isolates), to assess the physico-chemical characteristics of the biopolymers produced by these rhizobia and to determine the soluble intracellular proteins that are present in these rhizobia. The polysaccharides containing acetyl and pyruvic acid groups that were produced by different strains that had been cultivated in yeast extract mannitol (YEM) medium for 132, 144, and 168 h were evaluated for yield, viscosity, and concentration. Based on the analysis of their partial 16S rDNA sequences, both isolates were identified as Rhizobium tropici. The polymers produced in liquid YEM medium were recovered, dried and weighed to determine culture yield. Soluble intracellular proteins were identified through the techniques of 2D-PAGE and mass spectrometry for cultures that were cultivated for 168 h. The largest biopolymer yield and the highest viscosity and concentration of acetyl and pyruvic acids were obtained from the IPA 403 isolate after 168 h of culture. The proteins that were identified for the CIAT 899 isolate included elongation factor TU, a chaperone; GroE/GroEs and a putative glycosyltransferase, all of which catalyze the production of polysaccharides. For the IPA 403 strain, dinitrogenase and nitrogenase iron proteins were found. In the IPA 49 strain, glyceraldehyde-3-phosphate dehydrogenase was found along with two other proteins, the beta subunit of an electron-transferring flavoprotein and a dehydrogenase.

  12. Escherichia coli BdcA controls biofilm dispersal in Pseudomonas aeruginosa and Rhizobium meliloti

    Directory of Open Access Journals (Sweden)

    Wood Thomas K

    2011-10-01

    Full Text Available Abstract Background Previously we showed that BdcA controls Escherichia coli biofilm dispersal by binding the ubiquitous bacterial signal cyclic diguanylate (c-di-GMP; upon reducing the concentration of c-di-GMP, the cell shifts to the planktonic state by increasing motility, decreasing aggregation, and decreasing production of biofilm adhesins. Findings Here we report that BdcA also increases biofilm dispersal in other Gram-negative bacteria including Pseudomonas aeruginosa, Pseudomonas fluorescens, and Rhizobium meliloti. BdcA binds c-di-GMP in these strains and thereby reduces the effective c-di-GMP concentrations as demonstrated by increases in swimming motility and swarming motility as well as by a reduction in extracellular polysaccharide production. We also develop a method to displace existing biofilms by adding BdcA via conjugation from E. coli in mixed-species biofilms. Conclusion Since BdcA shows the ability to control biofilm dispersal in diverse bacteria, BdcA has the potential to be used as a tool to disperse biofilms for engineering and medical applications.

  13. Rhizobium Radiobacter Infection in a 27-Year-Old African American Woman With Munchausen Syndrome.

    Science.gov (United States)

    Sawhney, Sameer; Naab, Tammey; Oneal, Partricia

    2016-08-01

    Rhizobium radiobacter is an opportunistic, usually saprophytic, gram-negative bacillus found in agricultural soil. Isolation from blood has been reported most often in hospitalized patients harboring malignant neoplasms or human immunodeficiency virus (HIV) associated immunosuppression, who have catheter or medical device-related febrile neutropenia; treatment involves removal of the catheter or implanted medical device.(1)Herein, we report a case of a 27-year-old African American woman with sickle cell anemia who sought treatment of generalized body pain, shaking, chills, dyspnea, and fever, suggestive of sickle cell crisis. As part of her work up, routine blood cultures were drawn, revealing the presence of a Gram negative bacillus that was identified as the nonfermenter bacillus R. radiobacter The patient displayed a unique infection with R. radiobacter sepsis in a patient secondary to self-injection of organic material into a peripheral line during hospitalization. The growth of an unusual organism in the blood of a patient, without the usual risk factors of R. radiobacter, raised suspicion of a factitious psychiatric disorder known as Munchausen syndrome, which was confirmed when we discovered self-injection of feces and dirt into a central intravenous (IV) line. PMID:27107290

  14. Molecular phylogenetic analysis of Rhizobium sullae isolated from Algerian Hedysarum flexuosum.

    Science.gov (United States)

    Aliliche, Khadidja; Beghalem, Hamida; Landoulsi, Ahmed; Chriki, Ali

    2016-07-01

    Isolates from root nodules of Hedysarum flexuosum, sampled from north region of Algeria, were analyzed on the basis of their phenotypic and molecular characteristics. They were tested for their tolerance to NaCl, pH, temperatures, antibiotics and heavy metals resistance. Interestingly, the isolate Hf_04N appeared resistant to ZnCl2 (50 μg/mL) and grew at high saline concentration up to 9 %. The phylogenetic positions of five isolates were studied by comparative sequence analysis of 16S rRNA, recA, nifH and nodD genes. There were grouped close to the Rhizobium sullae type strain in relation to their 16S rRNA, recA and nifH genes-based phylogenies. By contrast, the tree of nodD gene was not congruent with ribosomal, housekeeping and nitrogen fixation genes. We suggest that our strains have a novel nodD gene. The detection of conserved domains of NodD protein and nitrogenase reductase enzyme, confirm their ability to nodulate and fix nitrogen. PMID:27034287

  15. Isolation of genes (nif/hup cosmids) involved in hydrogenase and nitrogenase activities in Rhizobium japonicum.

    Science.gov (United States)

    Hom, S S; Graham, L A; Maier, R J

    1985-03-01

    Recombinant cosmids containing a Rhizobium japonicum gene involved in both hydrogenase (Hup) and nitrogenase (Nif) activities were isolated. An R. japonicum gene bank utilizing broad-host-range cosmid pLAFR1 was conjugated into Hup- Nif- R. japonicum strain SR139. Transconjugants containing the nif/hup cosmid were identified by their resistance to tetracycline (Tcr) and ability to grow chemoautotrophically (Aut+) with hydrogen. All Tcr Aut+ transconjugants possessed high levels of H2 uptake activity, as determined amperometrically. Moreover, all Hup+ transconjugants tested possessed the ability to reduce acetylene (Nif+) in soybean nodules. Cosmid DNAs from 19 Hup+ transconjugants were transferred to Escherichia coli by transformation. When the cosmids were restricted with EcoRI, 15 of the 19 cosmids had a restriction pattern with 13.2-, 4.0-, 3.0-, and 2.5-kilobase DNA fragments. Six E. coli transformants containing the nif/hup cosmids were conjugated with strain SR139. All strain SR139 transconjugants were Hup+ Nif+. Moreover, one nif/hup cosmid was transferred to 15 other R. japonicum Hup- mutants. Hup+ transconjugants of six of the Hup- mutants appeared at a frequency of 1.0, whereas the transconjugants of the other nine mutants remained Hup-. These results indicate that the nif/hup gene cosmids contain a gene involved in both nitrogenase and hydrogenase activities and at least one and perhaps other hup genes which are exclusively involved in H2 uptake activity.

  16. Symbiont abundance is more important than pre-infection partner choice in a Rhizobium - legume mutualism.

    Science.gov (United States)

    Van Cauwenberghe, Jannick; Lemaire, Benny; Stefan, Andrei; Efrose, Rodica; Michiels, Jan; Honnay, Olivier

    2016-07-01

    It is known that the genetic diversity of conspecific rhizobia present in root nodules differs greatly among populations of a legume species, which has led to the suggestion that both dispersal limitation and the local environment affect rhizobial genotypic composition. However, it remains unclear whether rhizobial genotypes residing in root nodules are representative of the entire population of compatible symbiotic rhizobia. Since symbiotic preferences differ among legume populations, the genetic composition of rhizobia found within nodules may reflect the preferences of the local hosts, rather than the full diversity of potential nodulating rhizobia present in the soil. Here, we assessed whether Vicia cracca legume hosts of different provenances select different Rhizobium leguminosarum genotypes than sympatric V. cracca hosts, when presented a natural soil rhizobial population. Through combining V. cracca plants and rhizobia from adjacent and more distant populations, we found that V. cracca hosts are relatively randomly associated with rhizobial genotypes. This indicates that pre-infection partner choice is relatively weak in certain legume hosts when faced with a natural population of rhizobia. PMID:27269381

  17. Azide resistance in Rhizobium ciceri linked with superior symbiotic nitrogen fixation.

    Science.gov (United States)

    Bhaskar, V Vijay

    2004-12-01

    Isolated azide resistant (AzR) native R. ciceri strain 18-7 was resistant to sodium azide at 10 microg/ml. To find if nif-reiteration is responsible for azide resistance and linked to superior symbiotic nitrogen fixation, transposon (Tn5) induced azide sensitive mutants were generated. Using 4 kb nif-reiterated Sinorhizobium meliloti DNA, a clone C4 that complemented azide sensitivity was isolated by DNA hybridization from genomic library of chickpea Rhizobium strain Rcd301. EcoRI restriction mapping revealed the presence of 7 recognition sites with a total insert size of 19.17 kb. Restriction analysis of C4 clone and nif-reiterated DNA (pRK 290.7) with EcoRI and XhoI revealed similar banding pattern. Wild type strain 18-7, mutant M126 and complemented mutant M126(C4) were characterized for symbiotic properties (viz., acetylene reduction assay, total nitrogen content, nodule number and fresh and dry weight of the infected plants) and explanta nitrogenase activity. Our results suggested that azide resistance, nif-reiteration, and superior symbiotic effectiveness were interlinked with no correlation between ex-planta nitrogenase activity and azide resistance in R. ciceri.

  18. Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants

    Directory of Open Access Journals (Sweden)

    Hatice Öğütcü

    2014-04-01

    Full Text Available In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia Erzurum belongs to the high altitude (2000 - 2500m regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill were collected during the months of June and July. Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days. Colonies appear after incubation typically constitute (white, clear or slightly opaque, mucosity, round, raised 39 isolates were selected and transferred to tubes and refrigerated YMA were stored at +4ºC. In the next stage, cytological and biochemical analyzes of these isolates were studied to determine. For this purpose isolates; YMA containing bromothymol blue and congo red reproduction, gram stain reaction, movement and subjected to catalase and oxidase tests were evaluated. The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.

  19. Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Ormeño-Orrillo Ernesto

    2012-12-01

    Full Text Available Abstract Background Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 are α-Proteobacteria that establish nitrogen-fixing symbioses with a range of legume hosts. These strains are broadly used in commercial inoculants for application to common bean (Phaseolus vulgaris in South America and Africa. Both strains display intrinsic resistance to several abiotic stressful conditions such as low soil pH and high temperatures, which are common in tropical environments, and to several antimicrobials, including pesticides. The genetic determinants of these interesting characteristics remain largely unknown. Results Genome sequencing revealed that CIAT 899 and PRF 81 share a highly-conserved symbiotic plasmid (pSym that is present also in Rhizobium leucaenae CFN 299, a rhizobium displaying a similar host range. This pSym seems to have arisen by a co-integration event between two replicons. Remarkably, three distinct nodA genes were found in the pSym, a characteristic that may contribute to the broad host range of these rhizobia. Genes for biosynthesis and modulation of plant-hormone levels were also identified in the pSym. Analysis of genes involved in stress response showed that CIAT 899 and PRF 81 are well equipped to cope with low pH, high temperatures and also with oxidative and osmotic stresses. Interestingly, the genomes of CIAT 899 and PRF 81 had large numbers of genes encoding drug-efflux systems, which may explain their high resistance to antimicrobials. Genome analysis also revealed a wide array of traits that may allow these strains to be successful rhizosphere colonizers, including surface polysaccharides, uptake transporters and catabolic enzymes for nutrients, diverse iron-acquisition systems, cell wall-degrading enzymes, type I and IV pili, and novel T1SS and T5SS secreted adhesins. Conclusions Availability of the complete genome sequences of CIAT 899 and PRF 81 may be exploited in further efforts to understand the interaction of tropical

  20. Genetic and structural analysis of the Rhizobium meliloti fixA, fixB, fixC, and fixX genes.

    OpenAIRE

    Earl, C D; Ronson, C W; Ausubel, F M

    1987-01-01

    The fixA, fixB, fixC, and fixX genes of Rhizobium meliloti 1021 constitute an operon and are required for nitrogen fixation in alfalfa nodules. DNA homologous to the R. meliloti fixABC genes is present in all other Rhizobium and Bradyrhizobium species examined, but fixABC-homologous sequences were found in only one free-living diazotroph, Azotobacter vinelandii. To determine whether the fixABCX genes share sequence homology with any of the 17 Klebsiella pneumoniae nif genes, we determined the...

  1. Two-dimensional proteome reference map of Rhizobium tropici PRF 81 reveals several symbiotic determinants and strong resemblance with agrobacteria.

    Science.gov (United States)

    Gomes, Douglas Fabiano; Batista, Jesiane Stefania da Silva; Torres, Adalgisa Ribeiro; de Souza Andrade, Diva; Galli-Terasawa, Lygia Vitoria; Hungria, Mariangela

    2012-03-01

    Rhizobium tropici strain PRF 81 is used in commercial inoculants for common-bean crops in Brazil because of its high efficiency in nitrogen fixation and, as in other strains belonging to this species, its tolerance of environmental stresses, representing a useful biological alternative to chemical nitrogen fertilizers. In this study, a proteomic reference map of PRF 81 was obtained by two-dimensional gel electrophoresis and MALDI-TOF/TOF-TOF mass spectrometry. In total, 115 spots representing 109 different proteins were successfully identified, contributing to a better understanding of the rhizobia-legume symbiosis and supporting, at proteomics level, a strong resemblance with agrobacteria.

  2. Isolation of Rhizobium phaseoli Tn5-induced mutants with altered expression of cytochrome terminal oxidases o and aa3.

    Science.gov (United States)

    Soberón, M; Membrillo-Hernández, J; Aguilar, G R; Sánchez, F

    1990-01-01

    Two Rhizobium phaseoli mutants affected in cytochrome expression were obtained by Tn5-mob mutagenesis of the wild-type strain (CE3). Mutant strain CFN031 expressed sevenfold less cytochrome o in culture, expressed cytochrome aa3 under microaerophilic culture conditions, in contrast to strain CE3, and was affected in its vegetative growth properties and proliferation inside plant host cells. Mutant CFN037 expressed cytochrome aa3 under microaerophilic culture conditions, while bacteroid development and nitrogen fixation occurred earlier than in strain CE3. Images FIG. 2 PMID:2155209

  3. Erwinia herbicola isolates from alfalfa plants may play a role in nodulation of alfalfa by Rhizobium meliloti.

    OpenAIRE

    Handelsman, J; Brill, W J

    1985-01-01

    Erwinia herbicola was isolated from roots of plants derived from surface-sterilized seeds of all alfalfa varieties that were tested. Some of these E. herbicola strains affected nodulation by certain strains of Rhizobium meliloti. In previously published work we presented the isolation of slow-and fast-nodulating variants from a single culture of R. meliloti 102F51. In the absence of E. herbicola, the slow-nodulating variant induced the formation of nodules on alfalfa as rapidly as the faster-...

  4. Characterization of genes involved in erythritol catabolism in Rhizobium leguminosarum bv. viciae.

    Science.gov (United States)

    Yost, Christopher K; Rath, Amber M; Noel, Tanya C; Hynes, Michael F

    2006-07-01

    A genetic locus encoding erythritol uptake and catabolism genes was identified in Rhizobium leguminosarum bv. viciae, and shown to be plasmid encoded in a wide range of R. leguminosarum strains. A Tn5-B22 mutant (19B-3) unable to grow on erythritol was isolated from a mutant library of R. leguminosarum strain VF39SM. The mutated gene eryF was cloned and partially sequenced, and determined to have a high homology to permease genes of ABC transporters. A cosmid complementing the mutation (pCos42) was identified and was shown to carry all the genes necessary to restore the ability to grow on erythritol to a VF39SM strain cured of pRleVF39f. In the genomic DNA sequence of strain 3841, the gene linked to the mutation in 19B-3 is flanked by a cluster of genes with high homology to the known erythritol catabolic genes from Brucella spp. Through mutagenesis studies, three distinct operons on pCos42 that are required for growth on erythritol were identified: an ABC-transporter operon (eryEFG), a catabolic operon (eryABCD) and an operon (deoR-tpiA2-rpiB) that encodes a gene with significant homology to triosephosphate isomerase (tpiA2). These genes all share high sequence identity to genes in the erythritol catabolism region of Brucella spp., and clustalw alignments suggest that horizontal transfer of the erythritol locus may have occurred between R. leguminosarum and Brucella. Transcription of the eryABCD operon is repressed by EryD and is induced by the presence of erythritol. Mutant 19B-3 was impaired in its ability to compete against wild-type for nodulation of pea plants but was still capable of forming nitrogen-fixing nodules.

  5. Production of Poly &beta-Hydroxybutyric Acid (PHB by Rhizobium elti and Pseudomonas stutzeri

    Directory of Open Access Journals (Sweden)

    Elsayed B. Belal

    2013-11-01

    Full Text Available The amount of chemosynthetic plastic waste increases every year and exact time for its degradation is unknown. Two poly &beta-Hydroxybutyric producing bacteria from different microbial sources were isolated and characterized for their morphological, biochemical properties. Based on their 16S rDNA, they were identified as Rhizobium elti E1 and Pseudomonas stutzeri E114. The bacterial strains were screened for PHB production and compared for the intensity of fluorescence using Nile red staining methods. PHB production conditions were optimized with different carbon and nitrogen sources, the highest PHB production was observed with mannitol, sucrose and ammonium sulphate by R. elti and P. stutzeri, respectively. Regarding incubation time as well as temperature and pH, optimum PHB production conditions were 48 h, 30C and 7, respectively. R. elti P. stutzeri are capable of accumulating appreciable levels of PHB from glucose, xylose, lactose, whey, molasses, sugar cane bagasse, rice straw hydroysate when 2% from all substrates were used an alternative carbon for the PHB production. Ammonium sulphate was the best nitrogen source. C/N ratio was also one of the factors that affected the production of PHB. The ratio of C/N that reaches 20:1 was considered the best ratio to produce the highest production of PHB. The highest yield of PHB was done by P. stutzeri more than R. elti. The present study provide the useful data about the optimized conditions for PHB production by R. elti and P. stutzeri that can be utilized for industrial production of PHB, a fast emerging alternative of non biodegradable plastics.

  6. Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciae

    Directory of Open Access Journals (Sweden)

    Krehenbrink Martin

    2008-01-01

    Full Text Available Abstract Background Proteins secreted by bacteria play an important role in infection of eukaryotic hosts. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial symbiosis. Proteins secreted during the infection process by some rhizobial strains can influence infection and modify the plant defence signalling pathways. The aim of this study was to systematically analyse protein secretion in the recently sequenced strain Rhizobium leguminosarum bv. viciae 3841. Results Similarity searches using defined protein secretion systems from other Gram-negative bacteria as query sequences revealed that R. l. bv. viciae 3841 has ten putative protein secretion systems. These are the general export pathway (GEP, a twin-arginine translocase (TAT secretion system, four separate Type I systems, one putative Type IV system and three Type V autotransporters. Mutations in genes encoding each of these (except the GEP were generated, but only mutations affecting the PrsDE (Type I and TAT systems were observed to affect the growth phenotype and the profile of proteins in the culture supernatant. Bioinformatic analysis and mass fingerprinting of tryptic fragments of culture supernatant proteins identified 14 putative Type I substrates, 12 of which are secreted via the PrsDE, secretion system. The TAT mutant was defective for the symbiosis, forming nodules incapable of nitrogen fixation. Conclusion None of the R. l. bv. viciae 3841 protein secretion systems putatively involved in the secretion of proteins to the extracellular space (Type I, Type IV, Type V is required for establishing the symbiosis with legumes. The PrsDE (Type I system was shown to be the major route of protein secretion in non-symbiotic cells and to secrete proteins of widely varied size and predicted function. This is in contrast to many Type I systems from other bacteria, which typically secrete specific substrates encoded by genes often localised in close proximity to

  7. 75 FR 62858 - United States, et al.

    Science.gov (United States)

    2010-10-13

    ... Antitrust Division United States, et al. v. American Express Company, et al.; Proposed Final Judgment and... been filed with the United States District Court for the Eastern District of New York in United States..., 2010, the United States and seven States filed a Complaint alleging that certain rules, policies,...

  8. Genome Sequences of the Lignin-Degrading Pseudomonas sp. Strain YS-1p and Rhizobium sp. Strain YS-1r Isolated from Decaying Wood

    OpenAIRE

    Prabhakaran, Madhu; Couger, Matthew B.; Jackson, Colin A.; Weirick, Tyler; Fathepure, Babu Z.

    2015-01-01

    Pseudomonas sp. strain YS-1p and Rhizobium sp. strain YS-1r were isolated from a lignin-degrading enrichment culture. The isolates degraded lignin-derived monomers, dimers, alkali lignin, and, to a smaller extent (3% to 5%), lignin in switch grass and alfalfa. Genome analysis revealed the presence of a variety of lignin-degrading genes.

  9. VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots

    DEFF Research Database (Denmark)

    Vijn, I; Yang, W C; Pallisgård, N;

    1995-01-01

    We isolated ENOD5, ENOD12 and ENOD40 homologues from Vicia sativa and studied their expression pattern during Rhizobium-induced nodule formation. Comparison of the VsENOD40 nucleotide sequence with the pea, soybean and alfalfa ENOD40 sequences showed that the sequences contain two conserved regions...

  10. Growth, nodulation and yield of mash bean (Vigna mungo L. as affected by Rhizobium inoculation and soil applied L-tryptophan

    Directory of Open Access Journals (Sweden)

    Muhammad Iqbal Hussain*, Muhammad Javed Akhtar, Hafiz Naeem Asghar and Maqshoof Ahmad

    2011-04-01

    Full Text Available Most of the grain legumes in Pakistan are poorly nodulated either because of low indigenous rhizobialpopulation or due to inefficient strains of native rhizobia. However, improvement in nodulation could be achievedthrough inoculation with effective rhizobial strains. A pot trial was conducted to evaluate the effect of Rhizobiuminoculation along with L-tryptophan (L-TRP, a precursor of indole acetic acid (IAA on growth, yield andnodulation of mash been. The results indicated that Rhizobium inoculation along with L-tryptophan application (6mg kg-1 soil significantly improved plant height (44.6% as compared to control. Different improvements in rootlength (72.4%, oven dry root weight (Five fold, no. of pods plant-1 (86.4%, no. of grains per pod (42.8%, 100-grain weight (18.9% and nitrogen concentration in grains (two fold, was noted as compared with the controlwhere L-TRP was applied @ 2 mg kg-1 soil. There was a significant increase in number of nodules plant-1 (one fold,nodule fresh weight plant-1 (two fold and nodule dry weight plant-1 (four fold with Rhizobium inoculation alongwith L-TRP application @ 2 mg kg-1 soil as compared to Rhizobium inoculation alone. The study showed thatRhizobium inoculation along with L-TRP application could be a better approach for sustainable legume production.

  11. Genetic Factors in Rhizobium Affecting the Symbiotic Carbon Costs of N2 Fixation and Host Plant Biomass Production

    DEFF Research Database (Denmark)

    Skøt, L.; Hirsch, P. R.; Witty, J. F.

    1986-01-01

    The effect of genetic factors in Rhizobium on host plant biomass production and on the carbon costs of N2 fixation in pea root nodules was studied. Nine strains of Rhizobium leguminosarum were constructed, each containing one of three symbiotic plasmids in combination with one of three different...... the lowest carbon costs of N2 fixation (7.10–8.10 μmol C/μmol N2), but shoot dry weight of those plants was also smaller than that of plants nodulated by strains with the background of B151 or JI8400. Nodules formed by these two strain types had carbon costs of N2 fixation varying between 11.26 and 13.......95 μmol C/μmol N2. The effect of symbiotic plasmids on the carbon costs was relatively small. A time-course experiment demonstrated that nodules formed by a strain derived from JI6015 were delayed in the onset of nitrogenase activity and had a lower rate of activity compared to nodules induced by a strain...

  12. Intensity of Ground Cover Crop Arachis pintoi, Rhizobium Inoculation and Phosphorus Application and Their Effects on Field Growth and Nutrient Status of Cocoa Plants

    Directory of Open Access Journals (Sweden)

    John Bako Baon

    2006-08-01

    Full Text Available Arachis pintoiis potentially as a cover crop for cocoa (Theobroma cacaoL. farm, however information regarding its effect on the growth of cocoa plants in the field is very limited. The objective of this experiment is to investigate the combined influence of ground cover crop A. pintoi, rhizobial bacterial inoculation and phosphorus (P fertilizer on the growth of cocoa in the field and nutrient status. This experiment laid out in split-split plot design consisted of three levels of cover crop (without, A. pintoiand Calopogonium caeruleum, two levels of rhizobium inoculation (not inoculated and inoculated and two levels of phosphorus application (no P added and P added. The results showed that in field condition the presence of A. pintoias cover crop did not affect the growth of cocoa. On the other hand, C. caeruleumas cover crop tended to restrict cocoa growth compared to A. pintoi. Application of P increased leaf number of cocoa plant. Biomass production of A. pintoiwas 40% higher than C. caeruleum. Soil organic carbon and nitrogen contents were not affected by ground cover crops, though higher value (0.235% N and 1.63% organic C was obtained from combined treatments of inoculation and P addition or neither inoculation nor P addition. In the case of no rhizobium inoculation, soil N content in cocoa farm with A. pintoicover crop was lower than that of without cover crop or with C. caeruleum. Cover crop increased plant N content when there was no inoculation, on the other hand rhizobium inoculation decreased N content of cocoa tissue. Tissue P content of cocoa plant was not influenced by A. Pintoicover crop or by rhizobium inoculation, except that the P tissue content of cocoa was 28% higher when the cover crop was C. caeruleumand inoculated. Key words : Arachis pintoi, Theobroma cacao, Calopogonium caeruleum, rhizobium, nitrogen, phosphorus.

  13. 大豆和花生根瘤菌氢酶的研究%Studies on Hydrogenase from Rhizobium japonicum and Rhizobium arachis

    Institute of Scientific and Technical Information of China (English)

    许良树; 张凤章; 龙敏南; 曾定; 黄河清; 刘月英; 刘广发

    2001-01-01

    根瘤菌在共生固氮过程中因放H2所消耗的能量约占固氮总能量的40%~60%.吸氢酶则能回收和利用固氮过程所放的H2,减少能量损失,从而提高共生固氮效率.在厌氧条件下,加入防止酶蛋白聚合的试剂,利用DEAE-纤维素和SephacrylS-200柱层析,从自养性大豆根瘤菌和花生根瘤菌类菌体中分离并提纯膜结合态氢酶.纯化的两种氢酶表现相近的分子特征:均含有大(60 kD,65kD)、小(30 kD,35 kD)两个亚基;均为NiFe-氢酶,并具有较高的吸H2活性.大豆根瘤菌氢酶的纯酶组分不含Cyt b559.花生根瘤菌L8-3菌株能进行化能自养生长,诱导出高吸H2活性.根瘤菌的吸H2能明显提高固氮活性.从具有高吸H2活性的花生根瘤菌中分离并克隆吸氢基因,采用PCR和探针杂交技术,获得含有吸氢基因的质粒pZ-55.利用多种限制性内切酶构建了质粒pZ-55的物理图谱.通过三亲本杂交,将含吸氢基因的重组质粒转移到不吸H2的花生和毛豆根瘤菌中,所获得的结合株在自生和共生条件下均表达吸H2活性.以结合株接种大田花生,获得的共生根瘤的吸H2活性比接种受体株提高4倍,花生叶片和种子的含N量、产量分别提高1.7%、8.9%和9.6%.%Hydrogen produced by nitrogenase consumed 40~60%0 of energy of symbiotic nitro gen-fixation. Hydrogenase can uptake and reuse the H2 produced by nitrogenase, which results in decreasing the loss of energy and increasing the efficiency of nitrogen fixation. The membranebound hydrogenase from autotrophical cultured Rhizobium japanicum and from the bacteroids of peanut nodule have been purified and characterized. The hydrogenase from R. japonicum consists of two subunits (60 kD, 30 kD). The molecular weight of large and small subunits of hydrogenase from R. arachis is about 65 kD and 35 kD. Both hydrogenases are NiFe-hydrogenase. No cytochrome b(559) could

  14. Acetyl Coenzyme A Acetyltransferase of Rhizobium sp. (Cicer) Strain CC 1192.

    Science.gov (United States)

    Kim, S A; Copeland, L

    1997-09-01

    To investigate why Rhizobium sp. (Cicer) strain CC 1192 cells accumulate poly-R-3-hydroxybutyrate in the free-living state but not as bacteroids in nodules on chickpea (Cicer arietinum L.) plants, we have examined the kinetic properties of acetyl coenzyme A (acetyl-CoA) acetyltransferase (also known as acetoacetyl-CoA thiolase and 3-ketothiolase [EC 2.3.1.9]) from both types of cells. The enzyme had a native molecular mass of 180 (plusmn) 4 kDa, and the subunit molecular mass was 44 (plusmn) 1 kDa. The seven amino acids from the N terminus were Lys-Ala-Ser-Ile-Val-Ile-Ala. Thiolysis and condensation activity of the enzyme from free-living CC 1192 cells were optimal at pHs 7.8 and 8.1, respectively. The relationship between substrate concentrations and initial velocity for the thiolysis reaction were hyperbolic and gave K(infm) values for acetoacetyl-CoA and CoA of 42 and 56 (mu)M, respectively. The maximum velocity in the condensation direction was approximately 10% of that of the thiolysis reaction. With highly purified preparations of the enzyme, a value of approximately 1 mM was determined for the apparent K(infm) for acetyl-CoA. However, with partially purified enzyme preparations or when N-ethylmaleimide was included in reaction mixtures the apparent K(infm) for acetyl-CoA was close to 0.3 mM. In the condensation direction, CoA was a potent linear competitive inhibitor with an inhibition constant of 11 (mu)M. The much higher affinity of the enzyme for the product CoA than the substrate acetyl-CoA could have significance in view of metabolic differences between bacteroid and free-living cells of CC 1192. We propose that in free-living CC 1192 cells, the acetyl-CoA/CoA ratio reaches a value that allows condensation activity of acetyl-CoA acetyltransferase, but that in CC 1192 bacteroids, the ratio is poised so that the formation of acetoacetyl-CoA is not favored.

  15. Entrepreneuriat et innovations organisationnelles : pratiques et principes

    OpenAIRE

    Fonrouge, Cecile

    2008-01-01

    Les innovations de structure, systèmes et pratiques de gestion participent à une dynamique de changement qui les rendent dignes d'intérêt en entrepreneuriat. Trois cas d'innovations internes sont étudiés : un réseau d'entreprises, un système de numérisation des données de conception et des pratiques visant à concilier l'exploration et l'exploitation du potentiel technologique. Les conclusions portent sur l'effet organisationnel des choix technologiques et la place essentielle des entrepreneur...

  16. Sesbania rostrata, légumineuse à nodules caulinaires : fixation symbiotique d'azote, et utilisation comme engrais vert

    OpenAIRE

    Moudiongui, A.

    1988-01-01

    Des souches de #Rhizobium$ isolées de #Sesbania rostrata$ provenant de différents lieux géographiques du Sénégal, ont été analysées. Leur comparaison avec la "souche de tige" de référence ORS571 a permis de mettre en évidence des différences au niveau des parentés immunologiques, du contenu plasmidique, de l'activité nitrogénase en condition de dérépression, et de l'efficience de la symbiose. L'azote combiné, ammoniacal ou nitrique affecte à la fois la nodulation et l'activité réductrice d'ac...

  17. Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The 660 bp region between nodD3 P1 promoter and the following coding region of Rhizobium meliloti has been studied.This region is designated "downstream sequences".It consists of two potential open reading frames,ORF1 and ORF2.Studies on the role of the downstream sequences on the activity of nodD3 P1 with nod D3(P1)-lacZ fusion show that deletion of the sequences containing ORF2 causes the increase of the activity of the fusion; on the contrary,addition of extra copies of ORF2 markedly decreases the activity of the fusion.These results indicate that the product of ORF2 plays a negative role in the expression of nod D3.

  18. Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae

    Institute of Scientific and Technical Information of China (English)

    Yinghua Zhang; Guofan Hong

    2009-01-01

    NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum by. viciae strain 8401/pRL1JI, the NifA gene is part of a gene cluster (fixABCXNifAB). In this study, results showed that in R. leguminosarum by. viciae 8401/pRLI1I, host factor required (Hfq), and RNase E were involved in the post-transcriptional regulation of NifA expression. It was found that Hfq-dependent RNase E cleavage of NifA mRNA was essen-tial for NifA translation. The cleavage site is located at 32 nucleotides upstream of the NifA translational start codon. A possible explanation based on predicted RNA secondary structure of the NifA 5'-untranslated region was that the cleavage made ribosome-binding sites accessible for translation.

  19. Mechanisms of protection of pea plants by polysaccharides extracted from a strain of Rhizobium against Orobanche crenata

    International Nuclear Information System (INIS)

    The Broomrape causes notable damage on the leguminous crops and became major factor limiting production of pea in the Mediterranean region. The effect of the polysaccharides extracted from P.SOM Rhizobium strain on the development of Orobanche crenata on pea was studied. The results showed that the lipopolysaccharides significantly reduce the infestation of pea by O. crenata. This limitation of infestation results from the reduction of seeds germination rates of the parasite resulting in reduction of the tubercles number on pea roots. Moreover, necrosis of orobanche before or after attachment on pea roots treated by LPS can explain this reduction of parasitism. A correlation was observed between the reduction of pea infection by the broomrape and the activation phenolic compounds pathway. This activation resulted to increase of two enzymes (peroxidase and polyphenoloxidase) activities these enzymes are implicated in plant defense. The results of our study showed that the LPS seem implied in the induction of pea resistance against the broomrape.

  20. Syndicats et associations

    OpenAIRE

    Bars, Loïc Le; Barthélémy, Martine; Bellanger, Emmanuel; Béroud, Sophie; Béthouart, Bruno; Boussion, Samuel; Brodiez, Axelle; Brouster, Pascale Le; Capdevielle, Jacques; Chatriot, Alain; Coffin, Jean-Christophe; Diebolt, Évelyne; Dreyfus, Michel; Faniel, Jean; Frajerman, Laurent

    2015-01-01

    Les syndicats sont et demeurent des associations dans la large acceptiondu terme. Ils émergent et se développent dans l'espace du travail où préexistent nombre d'associations, d'initiatives et d'intitulés divers. Syndicats et associations remplissent de surcroît, souvent, les mêmes fonctions et nourrissent, parfois, des objectifs similaires. L'ouvrage qui rassemble des contributions émanant d'historiens, de sociologues et de politistes s'essaie à analyser la diversité des relations qui se son...

  1. NrcR, a New Transcriptional Regulator of Rhizobium tropici CIAT 899 Involved in the Legume Root-Nodule Symbiosis.

    Science.gov (United States)

    Del Cerro, Pablo; Rolla-Santos, Amanda A P; Valderrama-Fernández, Rocío; Gil-Serrano, Antonio; Bellogín, Ramón A; Gomes, Douglas Fabiano; Pérez-Montaño, Francisco; Megías, Manuel; Hungría, Mariangela; Ollero, Francisco Javier

    2016-01-01

    The establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wild-type strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutant exhibited reduced capacity to induce the nodC gene in comparison to the wild-type CIAT 899. The finding of a new nod-gene regulator located in a non-symbiotic plasmid may reveal the existence of even more complex mechanisms of regulation of nodulation genes in R. tropici CIAT 899 that may be applicable to other rhizobial species. PMID:27096734

  2. Et redskab i formskaben

    DEFF Research Database (Denmark)

    Thomsen, Bente Dahl

    Et innovativt didaktisk projekt, der arbejder med at udvikle et redskab til brug ved undervisningen i formskaben underlagt et universitært system. Det betyder at tidligere praksisbaseret læring i værksteder er omlagt til forskningsbaseret undervisning i auditoriumlignende lokaler. Projektet hvile...

  3. The development of plasmid-free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC58.

    Science.gov (United States)

    Hynes, M F; Simon, R; Pühler, A

    1985-03-01

    Agrobacterium tumefaciens strains LBA275 and LBA290 were cured of their cryptic plasmid pAtC58 by the introduction of the Rhizobium meliloti plasmid pRme41a, which is incompatible with pAtC58. pRme41a and pTiC58, the resident Ti plasmid of LBA275, were subsequently eliminated by growth at supraoptimal temperature (40 degrees C). The resulting plasmid-free Agrobacterium strains, UBAPF1 and UBAPF2, have proved extremely useful for the study of Rhizobium plasmids. The loss of the cryptic plasmid pAtC58 has no effect on the tumor-forming ability of the Agrobacterium strains; when the Ti plasmid is present, normal tumors are formed on Kalanchoe daigremontiana. PMID:4001194

  4. N2-Fixation in Alfalfa (Medicago sativa L.) Seedlings and Rhizobium meliloti L. Grown in Vitro Under Salt and Drought Stresses

    OpenAIRE

    Mohamad, Ramzi Muhiddin

    1987-01-01

    Alfalfa, Medicago sativa L., cultivars, breeding lines and germplasm releases (populations) and Rhizobium meliloti L. strains that exhibit nitrogen fixation efficiency and tolerance to salinity and drought stresses should enhance seedling establishment, increase yields, reduce nitrogen dependency on petroleum-based nitrogen fertilizers, and allow wider use of irrigated lands in semiarid and a rid regions. In vitro experiments were conducted to determine relative salt (sodium chloride -- NaCl)...

  5. Assignment of Biochemical Functions to Glycosyl Transferase Genes Which Are Essential for Biosynthesis of Exopolysaccharides in Sphingomonas Strain S88 and Rhizobium leguminosarum

    OpenAIRE

    Pollock, Thomas J.; van Workum, Wilbert A. T.; Thorne, Linda; Mikolajczak, Marcia J.; Yamazaki, Motohide; Kijne, Jan W.; Armentrout, Richard W.

    1998-01-01

    Glycosyl transferases which recognize identical substrates (nucleotide-sugars and lipid-linked carbohydrates) can substitute for one another in bacterial polysaccharide biosynthesis, even if the enzymes originate in different genera of bacteria. This substitution can be used to identify the substrate specificities of uncharacterized transferase genes. The spsK gene of Sphingomonas strain S88 and the pssDE genes of Rhizobium leguminosarum were identified as encoding glucuronosyl-(β1→4)-glucosy...

  6. The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.

    OpenAIRE

    Becker, A.; Rüberg, S; Küster, H.; Roxlau, A A; Keller, M; Ivashina, T; H.P. Cheng; Walker, G C; Pühler, A

    1997-01-01

    Proteins directing the biosynthesis of galactoglucan (exopolysaccharide II) in Rhizobium meliloti Rm2011 are encoded by the exp genes. Sequence analysis of a 32-kb DNA fragment of megaplasmid 2 containing the exp gene cluster identified previously (J. Glazebrook and G. C. Walker, Cell 56:661-672, 1989) revealed the presence of 25 open reading frames. Homologies of the deduced exp gene products to proteins of known function suggested that the exp genes encoded four proteins involved in the bio...

  7. A Mannosyl Transferase Required for Lipopolysaccharide Inner Core Assembly in Rhizobium leguminosarum: Purification, substrate specificity, and expression in Salmonella waaC mutants*

    OpenAIRE

    Kanipes, Margaret I.; Ribeiro, Anthony A.; Lin, Shanhua; Cotter, Robert J.; Raetz, Christian R. H.

    2003-01-01

    The lipopolysaccharide (LPS) core domain of Gram-negative bacteria plays an important role in outer membrane stability and host interactions. Little is known about the biochemical properties of the glycosyltransferases that assemble the LPS core. We now report the purification and characterization of the Rhizobium leguminosarum mannosyl transferase LpcC, which adds a mannose unit to the inner 3-deoxy-D-manno-octulosonic acid (Kdo) moiety of the LPS precursor, Kdo2-lipid IVA. LpcC containing a...

  8. Final seminar of the 11A025 research project : Instrumentation and monitoring methods for civil engineering applications : Laboratoire Central des Ponts et Chaussées, Bouguenais, 14-15 décembre 2005

    OpenAIRE

    LABORATOIRE CENTRAL DES PONTS ET CHAUSSEES - LCPC

    2005-01-01

    Le contrôle de structure ainsi que les outils associés s'y rattachant prennent de plus en plus une solide et croissante importantce pour les utilisateurs des méthodes expérimentales dans le génie civil. Les systèmes de mesure servent non seulement dans le cadre du suivi de la pathologie d'un pont ou d'un comportement défectueux, mais également en tant que vrais systèmes automatisés de prise de décision ou d'outils pour le contrôle actif de structure. De nos jours, ces systèmes comportent les ...

  9. Study of vibrations and stabilization of linear collider final doublets at the sub-nanometer scale; Etude des vibrations et de la stabilisation a l'echelle sous-nanometrique des doublets finaux d'un collisionneur lineaire

    Energy Technology Data Exchange (ETDEWEB)

    Bolzon, B

    2007-11-15

    CLIC is one of the current projects of high energy linear colliders. Vertical beam sizes of 0.7 nm at the time of the collision and fast ground motion of a few nanometers impose an active stabilization of the final doublets at a fifth of nanometer above 4 Hz. The majority of my work concerned vibrations and active stabilization study of cantilever and slim beams in order to be representative of the final doublets of CLIC. In a first part, measured performances of different types of vibration sensors associated to an appropriate instrumentation showed that accurate measurements of ground motion are possible from 0.1 Hz up to 2000 Hz on a quiet site. Also, electrochemical sensors answering a priori the specifications of CLIC can be incorporated in the active stabilization at a fifth of nanometer. In a second part, an experimental and numerical study of beam vibrations enabled to validate the efficiency of the numerical prediction incorporated then in the simulation of the active stabilization. Also, a study of the impact of ground motion and of acoustic noise on beam vibrations showed that an active stabilization is necessary at least up to 1000 Hz. In a third part, results on the active stabilization of a beam at its two first resonances are shown down to amplitudes of a tenth of nanometer above 4 Hz by using in parallel a commercial system performing passive and active stabilization of the clamping. The last part is related to a study of a support for the final doublets of a linear collider prototype in phase of finalization, the ATF2 prototype. This work showed that relative motion between this support and the ground is below imposed tolerances (6 nm above 0.1 Hz) with appropriate boundary conditions. (author)

  10. Beaurepaire et Escavalon

    OpenAIRE

    Saly, Antoinette

    2014-01-01

    Lorsque Perceval sort chevalier de chez Gornemant de Goort, c'est pour rencontrer aussitôt l'aventure où se profilera son destin. A peine a-t-il retrouvé les grands bois familiers que sa chevauchée le mène à un chastel fort et bien seant (v. 1707). Au fur et à mesure qu'il approche, cependant, ne s'offre qu'un paysage désolé de lande et d'eau : Defors les murs n'avoit neant Fors mer et aive et terre gaste (vv. 1708-1709). Il franchit un pont chancelant et se heurte à une porte close. Ses cris...

  11. Say NO to ET.

    Science.gov (United States)

    Vanhoutte, P M

    2000-07-01

    The endothelial cells release both relaxing [nitric oxide (NO), endothelium-derived hyperpolarizing factor (EDHF), prostacyclin] and contracting factors [endoperoxides, thromboxane A(2), superoxide anions, endothelin-1 (ET)]. The production of ET is inhibited by NO. The latter also strongly opposes the direct effects of the former on vascular smooth muscle. With aging and vascular disease, the production of enothelial NO declines, and thus ET can be released, act and contribute to the symptoms.

  12. Sources et bibliographie

    OpenAIRE

    2015-01-01

    Sources manuscrites Archives nationales Il s’agit ici non pas d’un inventaire de tous les documents des Archives nationales concernant les dons et legs, mais d’une présentation des documents utilisés dans ce travail, et d’un tableau des grandes séries existantes. Série F3 : Administration communale F3 / II. Administration des communes. « Échange, concessions, partages des propriétés communales, dons et legs faits aux communes ». [Classement par département, et par communes à l’intérieur du dé...

  13. Conversion et conversation

    OpenAIRE

    Mary, André

    2013-01-01

    Il est temps de faire le point sur l'apport théorique et empirique de l'oeuvre majeure de J. et J. Comaroff, Of Revelation and Revolution, dont deux tomes sont désormais parus (le troisième est en attente) et qui reste largement méconnue du public français. Cette anthropologie historique de l'évangélisme colonial est centrée sur la rencontre entre les missionnaires non-conformistes d'origine britannique et les Tswana d'Afrique du Sud. Le paradigme de l'interaction coloniale appliqué à ...

  14. Texte, Contexte et Connaissance

    OpenAIRE

    Van Dijk, Teun A.

    2013-01-01

    Les structures situationnelles et sociétales ne peuvent pas affecter directement les processus mentaux de production et de compréhension des discours ; il faut un système mental médiateur, c’est-à-dire des modèles mentaux spécifiques (des modèles contextuels) qui représentent la “définition de la situation de communication” subjective des participants. Ces modèles mentaux contrôlent la production des structures et les dimensions du discours et s’assure que le discours est approprié à la situa...

  15. Et dansk hotell

    DEFF Research Database (Denmark)

    Baca, Susan; Busck, Ole Gunni

    Rapporten præsenterer resultatet af en undersøgelse af, hvorledes et hotel i Nordjylland fungerer som arbejdsplads for indvandrere af overvejende ikke-vestlig oprindelse. Der fokuseres på i hvilket omfang og gennem hvilke mekanismer arbejdsmiljjøet i bred forstand, herunder forhold i ledelsen...

  16. Tabac et maladie parodontale

    OpenAIRE

    Geerts, Sabine

    1996-01-01

    Le tabac est un facteur aggravant la parodontite. Beaucoup d'études cliniques ont été menées et ont souvent montré des résultats contradictoires. Cet article est une revue de littérature sur la relation entre le tabagisme et la parodontite. Peer reviewed

  17. SNAP E&T

    Science.gov (United States)

    Lower-Basch, Elizabeth

    2014-01-01

    This document provides an overview of Supplemental Nutrition Assistance Program Employment and Training (SNAP E&T). SNAP E&T is a funding source that allows states to provide employment and training and related supportive services to individuals receiving Supplemental Nutrition Assistance Program (SNAP, formerly known as food stamps)…

  18. Cultures et organisations

    NARCIS (Netherlands)

    Hofstede, G.; Hofstede, G.J.; Minkov, M.

    2010-01-01

    La coopération interculturelle, une question de survie Véritable atlas des valeurs culturelles, paru en 18 langues, Cultures et organisations est le fruit de plus de 40 ans de recherches menées dans plus de 100 pays. Il est aujourd'hui le livre de référence des chercheurs, universitaires et professi

  19. Cartographie et intelligence artificielle

    Directory of Open Access Journals (Sweden)

    Denise Pumain

    2005-11-01

    Full Text Available Le monde complexe dans lequel nous évoluons est aussi paradoxal : ainsi, que peut-on prédire quant à la diffusion du savoir faire des géographes en termes de cartographie et de visualisation de l’information géographique ? On voit surgir toujours plus d’instruments et d’occasions de se repérer et d’admirer des images de la terre, mais il n’est pas sûr que cela fasse progresser dans le public la connaissance des cartes et de leur interprétation. Prenons l’exemple du repérage, par GPS, et bient...

  20. Agronomies et agronomes

    OpenAIRE

    Landais, Étienne; Boulier, Fabien; Robin, Paul

    2013-01-01

    L’agronomie française vit probablement un moment très important pour son avenir et connaît de profondes mutations. Ainsi, le pôle d’enseignement supérieur et de recherche de Montpellier, héritier d’une longue tradition et fort de sa dimension internationale méditerranéenne et tropicale, se réorganise. La création d’une « Faculté agronomique » est prévue en 2006. La concentration remarquable d’unités de recherche, dont beaucoup sont mixtes, dessine un espace scientifique et technique original ...

  1. Musique et document sonore

    OpenAIRE

    Javault, Patrick

    2013-01-01

    Tirée d'une thèse en musicologie et en esthétique, cette enquête est d'abord remarquable par sa façon d'établir des regroupements et de définir son chemin de réflexion. Pierre-Yves Macé, lui-même musicien, compositeur et praticien des archives phonographiques, trouve des transversales pour éclairer l'emploi du document sonore dans les musiques savantes et expérimentales (en écartant sciemment la pop) - document sonore envisagé comme Autre du musical et qui peut également être dit « le réel de...

  2. Aristote et Pascal

    OpenAIRE

    Michon, Hélène

    2016-01-01

    La seule mention qui est faite d’Aristote dans les Pensées est celle du fragment 457 : On ne s’imagine Platon et Aristote qu’avec de grandes robes de pédants. C’étaient des gens honnêtes et comme les autres, riants avec leurs amis. Et quand ils se sont divertis à faire leurs Lois et leurs Politiques, ils l’ont fait en se jouant. (fr. 457) Occurrence qui nous laisse à penser que si Pascal avait dû intervenir dans la querelle entre les Anciens et les Modernes, il l’eût certainement fait en épo...

  3. Biologic fixation of nitrogen in irradiated rhizobium strips; Fixacao biologica do nitrogenio em estirpes de rizobianas irradiadas

    Energy Technology Data Exchange (ETDEWEB)

    Caribe, Rebeka Alves; Colaco, Waldeciro [Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear

    2002-07-01

    Native Rhizobium sp. and Bradyrhizobium sp. isolates from the root nodules of bean and cowpea were selected. Six isolates, and the SEMIA 4077 (R. leguminosarum bv. phaseolus) and SEMIA 6145 (Bradyrhizobium sp) strains used as references, were irradiated with ultraviolet light (R-uv) and gamma rays (R-{gamma}). The D{sub 37} values for the rhizobial strain SEMIA 4077 were 43 J.m{sup -2} (UV) and 32 Gy (R-{gamma}) and for the SEMIA 6145 were 45 J.m{sup -2} (UV) and 35 Gy (R-gamma). Through a greenhouse experiment the irradiated isolates were inoculated on bean (P. vulgaris L., cv. Princesa) and on cowpea [Vigna unguiculata, (L.) Walp, cv. IPA-206] seedlings, in an attempt to evaluate the sensitivity of the host plants, and possible effects on their nodulation. Differences in responses to nodulation due to the effect of irradiation were observed for the isolates tested. Significantly differences were observed only for nodules dry matter yield of the IPA-206 cultivar. Gamma irradiated treatment were statistically superior to treatments with ultraviolet light in relation. (author)

  4. Symbiotic Efficiency of Native and Exotic Rhizobium Strains Nodulating Lentil (Lens culinaris Medik. in Soils of Southern Ethiopia

    Directory of Open Access Journals (Sweden)

    Wondwosen Tena

    2016-02-01

    Full Text Available Lentil plays a major role in the food and nutritional security of low income Ethiopian families because of the high protein content of their seed; however, their productivity typically is low largely due to soil fertility limitations. Field and pot experiments were conducted during the 2011 cropping season to determine the effectiveness of Rhizobium strains on two cultivars of lentil in Southern Ethiopia. Six rhizobial inoculant treatments (four indigenous and two commercial inoculants, a nitrogen (N fertilizer treatment (50 kg·urea·ha−1 and an absolute control (non-inoculated non-fertilized were used. Inoculated plants produced significantly higher nodule number, nodule dry weight, grain yield and yield components than non-inoculated non-fertilized plants. Inoculation of field grown lentil with rhizobia strain Lt29 and Lt5 enhanced seed yield by 59% and 44%, respectively. Whereas urea fertilization enhanced yields by 40%. Similarly, grain yields were increased during the pot experiment by 92% and 67% over the control treatments by inoculation with Lt29 and Lt5, respectively. The highest levels of N fixation were achieved in plants inoculated with Lt29 (65.7% Ndfa. Both field and pot investigations indicate that inoculation of lentil with native rhizobial strains replace the need for inorganic N fertilization to optimize lentil yields.

  5. Change in morphological properties of root and aerial parts of chickpea under drought stress, arbuscular mycorrhizal fungi and rhizobium treatments

    Directory of Open Access Journals (Sweden)

    S. Morad

    2016-09-01

    Full Text Available To evaluate the effect of arbuscular mycorrhizal (AM fungi (Glomus mosseae and Glomus intraradices and Mesorhizobium Ciceri bacteria at three soil moisture levels [28% (field capacity, FC, 15% (-5 bar suction and 9% (-10 bar tension by weight] on morphological properties of root and aerial parts of chickpea, a greenhouse factorial experiment, arranged as a complete randomized design, was conducted in a sterilized soil. Results showed that moisture treatment had significance effect on number of pods, number of seeds, fresh and dry weight of root and aerial parts, plant height and root length and volume. Application of Mesorhizobium was effective on number of nodes, number of pods, number of seeds, fresh and dry weight of root and aerial parts, plant height, root length and volume. Glomus mosseae had significant effect on plant height. Interaction of moisture and AM fungi was significant on fresh and dry weight of root and aerial parts. Interaction effect of Mesorhizobium and soil moisture was only significant on number of nodes. In general, the highest fresh and dry weight of aerial parts (44.6 and 10.53 grams, respectively was obtained by inoculation of chickpea by rhizobium bacteria and Glomus mosseae at FC moisture level, and AM fungi Glomus mosseae was more efficient compared to Glomus intraradices in both drought stress and without stress conditions.

  6. Biological control of crown gall on grapevine and root colonization by nonpathogenic Rhizobium vitis strain ARK-1.

    Science.gov (United States)

    Kawaguchi, Akira

    2013-01-01

    A nonpathogenic strain of Rhizobium vitis ARK-1 was tested as a biological control agent for grapevine crown gall. When grapevine roots were soaked in a cell suspension of strain ARK-1 before planting in the field, the number of plants with tumors was reduced. The results from seven field trials from 2009 to 2012 were combined in a meta-analysis. The integrated relative risk after treatment with ARK-1 was 0.15 (95% confidence interval: 0.07-0.29, P0.001), indicating that the disease incidence was significantly reduced by ARK-1. In addition, the results from four field trials from 2007 to 2009 using R. vitis VAR03-1, a previously reported biological control agent for grapevine crown gall, were combined in a meta-analysis. The integrated relative risk after treatment with VAR03-1 was 0.24 (95% confidence interval: 0.11-0.53, P0.001), indicating the superiority of ARK-1 in inhibiting grapevine crown gall over VAR03-1 under field conditions. ARK-1 did not cause necrosis on grapevine shoot explants. ARK-1 established populations on roots of grapevine tree rootstock and persisted inside roots for two years.

  7. Genetic diversity of a natural population of Rhizobium leguminosarum bv. viciae nodulating plants of Vicia faba in the Vesuvian area.

    Science.gov (United States)

    Ventorino, Valeria; Chiurazzi, Mario; Aponte, Maria; Pepe, Olimpia; Moschetti, Giancarlo

    2007-12-01

    A total of 98 rhizobial strains, isolated during the winter of the years 2003 (35 isolates), 2004 (33 isolates), and 2005 (30 isolates) were analyzed to determine the genetic diversity of the natural population nodulating Vicia faba plants and to identify dominant genotypes. All isolates were identified as Rhizobium leguminosarum bv. viciae by biovar-specific polymerase chain reaction amplification of the nodC gene. Intraspecific DNA polymorphism was evaluated through the restriction endonucleases analysis combined with pulsed-field gel electrophoresis. Four genotypes characterized 53% of the isolates, showing a high occurrence; moreover, they were recovered over the 3 years, thus showing a lasting persistence in the soil, which could mean a high degree of saprophytic competitiveness. The richness, diversity, and dominance indexes of genotypes were calculated to monitor the evolution of the rhizobial population during the 3 years. The genetic diversity of the analyzed strains decreased along the 3 years. In fact, the biodiversity index H' decreased from 2.6 in the first and second year to 1.9 in the third year; probably, as a result of bean monocropping, specific genotypes of Rh. leguminosarum bv. viciae were naturally selected. PMID:17899266

  8. Flocculating Properties and Production of the Compound Bioflocculant by Rhizobium Radiobacter F2 and Bacillus Sphaeicus F6

    Institute of Scientific and Technical Information of China (English)

    Lixin Li; Lingyan Feng; Fang Ma; Qianshen Zhao

    2015-01-01

    A compound bioflocculant CBF, produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6, was investigated with regard to its production and flocculating properties. The optimization of the culture medium constituents including carbon source, nitrogen source and C/N ratio, metal ions and ionic strength on CBF production were studied. Flocculating properties of CBF were examined by a series of experiments and CBF had good flocculating activities in kaolin suspension with divalent cations and stable over wide range of pH. Studies of the flocculating properties revealed that the flocculation could be stimulated by cations Ca2+, Mg2+, Fe2+, Al3+and Fe3+. In addition, it was stable at 4-30℃ in the presence of CaCl2 . It was found to be effective for flocculation of a kaolin suspension under neutral and weak alkaline conditions ( pH 7�0-9�0 ) , and flocculating activities of higher than 95% were obtained when the CBF concentrations among 6-14 mg/L at pH 8�0. The results of this study indicate that CBF is a potential replacement of conventional synthetic flocculants and is widely applied in water treatment and downstream processing of food and fermentation industries.

  9. Relevance of fucose-rich extracellular polysaccharides produced by Rhizobium sullae strains nodulating Hedysarum coronarium l. legumes.

    Science.gov (United States)

    Gharzouli, Razika; Carpéné, Marie-Anne; Couderc, François; Benguedouar, Ammar; Poinsot, Véréna

    2013-03-01

    Specific and complex interactions between soil bacteria, known as rhizobia, and their leguminous host plants result in the development of root nodules. This process implies a complex dialogue between the partners. Rhizobia synthesize different classes of polysaccharides: exopolysaccharides (EPS), Kdo-rich capsular polysaccharides, lipopolysaccharides, and cyclic β-(1,2)-glucans. These polymers are actors of a successful symbiosis with legumes. We focus here on studying the EPS produced by Rhizobium sullae bacteria that nodulate Hedysarum coronarium L., largely distributed in Algeria. We describe the influence of the carbon source on the production and on the composition of EPS produced by R. sullae A6 and RHF strains. High-molecular-weight EPS preserve the bacteria from desiccation. The structural characterization of the EPS produced by R. sullae strains has been performed through sugar analysis by gas chromatography-mass spectrometry. The low-molecular-weight EPS of one strain (RHF) has been totally elucidated using nuclear magnetic resonance and quantitative time-of-flight tandem mass spectrometry analyses. An unusual fucose-rich EPS has been characterized. The presence of this deoxy sugar seems to be related to nodulation capacity.

  10. Characterisation of SalRAB a salicylic acid inducible positively regulated efflux system of Rhizobium leguminosarum bv viciae 3841.

    Science.gov (United States)

    Tett, Adrian J; Karunakaran, Ramakrishnan; Poole, Philip S

    2014-01-01

    Salicylic acid is an important signalling molecule in plant-microbe defence and symbiosis. We analysed the transcriptional responses of the nitrogen fixing plant symbiont, Rhizobium leguminosarum bv viciae 3841 to salicylic acid. Two MFS-type multicomponent efflux systems were induced in response to salicylic acid, rmrAB and the hitherto undescribed system salRAB. Based on sequence similarity salA and salB encode a membrane fusion and inner membrane protein respectively. salAB are positively regulated by the LysR regulator SalR. Disruption of salA significantly increased the sensitivity of the mutant to salicylic acid, while disruption of rmrA did not. A salA/rmrA double mutation did not have increased sensitivity relative to the salA mutant. Pea plants nodulated by salA or rmrA strains did not have altered nodule number or nitrogen fixation rates, consistent with weak expression of salA in the rhizosphere and in nodule bacteria. However, BLAST analysis revealed seventeen putative efflux systems in Rlv3841 and several of these were highly differentially expressed during rhizosphere colonisation, host infection and bacteroid differentiation. This suggests they have an integral role in symbiosis with host plants.

  11. Characterisation of SalRAB a salicylic acid inducible positively regulated efflux system of Rhizobium leguminosarum bv viciae 3841.

    Directory of Open Access Journals (Sweden)

    Adrian J Tett

    Full Text Available Salicylic acid is an important signalling molecule in plant-microbe defence and symbiosis. We analysed the transcriptional responses of the nitrogen fixing plant symbiont, Rhizobium leguminosarum bv viciae 3841 to salicylic acid. Two MFS-type multicomponent efflux systems were induced in response to salicylic acid, rmrAB and the hitherto undescribed system salRAB. Based on sequence similarity salA and salB encode a membrane fusion and inner membrane protein respectively. salAB are positively regulated by the LysR regulator SalR. Disruption of salA significantly increased the sensitivity of the mutant to salicylic acid, while disruption of rmrA did not. A salA/rmrA double mutation did not have increased sensitivity relative to the salA mutant. Pea plants nodulated by salA or rmrA strains did not have altered nodule number or nitrogen fixation rates, consistent with weak expression of salA in the rhizosphere and in nodule bacteria. However, BLAST analysis revealed seventeen putative efflux systems in Rlv3841 and several of these were highly differentially expressed during rhizosphere colonisation, host infection and bacteroid differentiation. This suggests they have an integral role in symbiosis with host plants.

  12. C. Petrone et al.: "Magnetic measurement of the model magnet QD0 designed for the CLIC final focus beam transport line." CERN TE-MSC Internal Note, EDMS Nr: 1184196

    CERN Document Server

    Arpaia, Pasquale; Petrone, Carlo; Russenschuck, Stephan; Walckiers, Louis

    2012-01-01

    This note presents the results of the magnetic measurements performed on QD0, model magnet for the final focus transport line for CLIC (Fig. 1). This high-gradient, hybrid quadrupole has a yoke length of 0.1 m and an aperture of 8.3 mm. ND2Fe14B Permanent magnet blocks provide a gradient of 150 T/m, which can be further increased to 530 T/m when the four coils are excited to 18.3 A. The request was to measure the strength of the field and the multipole coefficients at different currents. The measurement of the field strength, by means of the single stretched wire system, was done in December 2011 in the I8 laboratory. The measurement of the multipole was done by means of the oscillating wire system [1][2].

  13. Final environmental statement related to the operation of Catawba Nuclear Station, Units 1 and 2. Docket Nos. 50-413 and 50-414, Duke Power Company, et al

    International Nuclear Information System (INIS)

    This Final Environmental Statement contains the second assessment of the environmental impact associated with the operation of the Catawba Nuclear Station, Units 1 and 2, pursuant to the National Environmental Policy Act of 1969 (NEPA) and 10 CFR 51, as amended, of the NRC regulations. This statement examines: the affected environment, environmental consequences and mitigating actions, and environmental and economic benefits and costs. Land use and terrestrial and aquatic-ecological impacts will be small. Operational impacts to historic and archeological sites will be negligible. The effects of routine operations, energy transmission, and periodic maintenance of rights-of-way and transmission facilities should not jeopardize any populations of endangered or threatened species. No significant impacts are anticipated from normal operational releases of radioactivity. The risk associated with accidental radiation exposure is very low. The net socioeconomic effects of the project will be beneficial

  14. Symbiotic effectiveness and ecological characterization of indigenous Rhizobium loti populations in Uruguay Eficiência simbiótica e caracterização ecológica de uma população nativa de Rhizobium loti no Uruguai

    OpenAIRE

    Amalia Baraibar; Llillian Frioni; Maria Elena Guedes; Hans Ljunggren

    1999-01-01

    The objectives of this work were to describe the distribution, density and seasonal variation of the indigenous populations of Rhizobium loti in different Uruguayan soils and to determine the symbiotic effectiveness and stress tolerance factors of different isolates, both with the aim of obtaining selected strains to re-introduce as inoculants in Lotus pastures. R. loti was present in ten soils studied and their densities varied from year to year and within each soil. All the isolates nodulat...

  15. Accumulation of a nod Gene Inducer, the Flavonoid Naringenin, in the Cytoplasmic Membrane of Rhizobium leguminosarum biovar viciae Is Caused by the pH-Dependent Hydrophobicity of Naringenin

    OpenAIRE

    Recourt, Kees; Brussel, Anton A.N. van; Driessen, Arnold J. M.; Lugtenberg, Ben J. J.

    1989-01-01

    Most Sym plasmid-localized nodulation genes of Rhizobium leguminosarum bv. viciae are only expressed upon activation of the NodD protein by plant flavonoids, e.g., naringenin (S. A. J. Zaat, C. A. Wijffelman, H. P. Spaink, A. A. N. van Brussel, and B. J. J. Lugtenberg, J. Bacteriol, 169:198-204, 1987). As part of a study on the mechanism of NodD protein activation, the mechanism of uptake and the intracellular fate of [3H]naringenin were studied. Naringenin was accumulated by Rhizobium cells ...

  16. Familles et immigration

    OpenAIRE

    Streiff-Fénart, Jocelyne

    2004-01-01

    A l'ère de la mondialisation, le devenir encore incertain et variable selon les groupes de l'immigration familiale se noue dans les rapports complexes entre des redéfinitions identitaires qui se jouent sur la scène culturelle mondialisée, et l'intégration des descendants des immigrés dans l'ordre national, qui se joue sur le terrain socio-économique et politique de l'égalisation des chances.

  17. Musique et politique

    OpenAIRE

    Albertini, Françoise; Bidart, Pierre; Capitolin, Jean-Louis; Cooper, Carolyn; Darré, Alain; Defrance, Yves; Dutertre, Jean-François; Guilbault, Jocelyne; Gumplowicz, Philippe; Hennion, Antoine; Herzhaft, Gérard; Leblon, Bernard; Liverpool, Hollis; Madiot, Béatrice; Maliesky, Dominique

    2015-01-01

    Musiciens et chanteurs sont à l'origine de pratiques qui s'insèrent pleinement dans le jeu complexe des rapports sociaux. Aux stades de la création, de la production ou de la consommation, les musiques n'échappent jamais aux enjeux socio-culturels d'un lieu, d'une époque, d'une communauté humaine. Conformes ou dissidentes à l'égard des logiques dominantes, elles demeurent productrices d'émotions esthétiques et porteuses des liens sociaux. Les musiques sont des enjeux de pouvoir et certaines l...

  18. Parler et convaincre

    CERN Document Server

    Vigner, Gérard

    1979-01-01

    Dans l'apprentissage d'une langue, l'échange oral est avant tout utilitaire. On demande un renseignement, un prix, on salue, on remercie... On peut aussi parler pour exposer un point de vue, se justifier, entraîner une décision : parler pour convaincre. Cet ouvrage de la collection OUTILS veut aider les élèves à ce type d'intervention ; il leur fait d'abord analyser la situation de communication dans laquelle ils doivent intervenir, puis trouver les mots et expressions justes et efficaces. Sa structure et ses exercices permettent un travail tant collectif qu'individuel.

  19. “Item perrexil, mostarda, lechugas et rauanos”. Notas sobre la alimentación de mineros alemanes en Pamplona a finales del siglo XIV (1392

    Directory of Open Access Journals (Sweden)

    Serrano Larráyoz, Fernando

    2008-06-01

    Full Text Available The present article represents an approximation to the food of some German miners settled in Pamplona at the end of the 14th century, to carry out a series of assays intended to determine the kind of metals present in the ore and their actual proportion, with the purpose to verify the profitability of some seams found at Five Villages. Their food accounting for approximately one and a half months, give us first hand information of their habits and of those sharing the table with them. To a certain extent, it is possible to extrapolate these practices to the ones of part of Navarra's salary earners. In them, we could sense certain customs or preferences characteristic of North Europe.

    El presente artículo es una aproximación a la alimentación de unos mineros alemanes que, a finales del siglo XIV, se asientan en Pamplona con el fin de realizar una serie de ensayos (averiguación de los metales que contiene la mena —el mineral metalífero—, y la proporción en que cada uno está con el peso de ella en los que determinar la rentabilidad de algunos filones mineros encontrados en la zona de las Cinco Villas. Las cuentas de los gastos que realizan en alimentación, en aprox. mes y medio, nos acercan de primera mano a los hábitos de estos hombres y de quienes les acompañan en la mesa. Unas prácticas que es posible extrapolar, en cierta medida, a parte de la menestralía navarra, y en las que podemos intuir ciertas costumbres (o gustos propios de la Europa del Norte.

  20. ADN et chromosomes

    OpenAIRE

    Hayes, Hélène

    2000-01-01

    Chaque chromosome contient une seule molécule d’ADN. L’ADN déroulé d’un noyau de cellule humaine mesurerait environ 1,8 m : chaque molécule d’ADN est enroulée et compactée en plusieurs étapes, grâce à l’association de différentes protéines, et loge dans le noyau de 6 µm de diamètre. Le degré de condensation de l’ADN est variable selon les régions chromosomiques et les régions les moins condensées sont les plus riches en gènes. L’ADN est composé d’une variété de séquences codantes ou non et ré...

  1. Algorithmes, machines et langages

    OpenAIRE

    Berry, Gérard

    2014-01-01

    Enseignement : Le temps et les événements en informatique J’ai donné le cours « Le temps et les événements en informatique » dans le cadre de la chaire Algorithmes, machines et langages, créée le 4 juillet 2013 comme première chaire de plein exercice en informatique. J’avais introduit l’informatique au Collège de France en 2007-2008 par le cours « Pourquoi et comment le monde devient numérique », au sein de la chaire annuelle d’Innovation technologique Liliane Bettencourt, puis, en 2009-2010,...

  2. Jeunes et Musique

    OpenAIRE

    Mauger, Gérard; Mignon, Patrick; Boyer, Régine; Hersent, Jean-François; Green, Anne-Marie; Daphy, Eliane

    1987-01-01

    Les Cahiers Jeunesses et sociétés sont une publication du Réseau « Jeunesses et Sociétés ». Le thème « Jeunes et Musiques » a été abordé dans le cadre des journées d'études du 26 juin 1986, 25 novembre 1986, et 26 mai 1987. Ce dixième numéro des Cahiers rend compte pour partie de ces travaux.SOMMAIRE• Présentation, par Gérard Mauger (1)• La production sociale du rock, par Patrick Mignon (3-32)• Les goûts musicaux des lycéens , par Régine Boyer (33-47)• Rock, jeunesse, socialisation. Jalons d'...

  3. A 13C-NMR study of exopolysaccharide synthesis in Rhizobium meliloti Su47 strain

    Science.gov (United States)

    Tavernier, P.; Portais, J.-C.; Besson, I.; Courtois, J.; Courtois, B.; Barbotin, J.-N.

    1998-02-01

    Metabolic pathways implied in the synthesis of succinoglycan produced by the Su47 strain of R. meliloti were evaluated by 13C-NMR spectroscopy after incubation with [1{-}13C] or [2{-}13C] glucose. The biosynthesis of this polymer by R. meliloti from glucose occurred by a direct polymerisation of the introduced glucose and by the pentose phosphate pathway. Les voies métaboliques impliquées dans la synthèse du succinoglycane produit par la souche Su47 de R. meliloti ont été évaluées par la spectroscopie de RMN du carbone 13 après incubation des cellules avec du [1{-}13C] ou [2{-}13C] glucose. La biosynthèse de ce polymère à partir du glucose se produit par polymérisation directe du glucose et par la voie des pentoses phosphate.

  4. LYSnET

    DEFF Research Database (Denmark)

    Bønløkke Andersen, Kätte; Hansen, Ellen Katrine; Krogsbæk Mathiasen, Nanet

    2006-01-01

    LYSnET.com skal bidrage til at udbrede og udvikle viden om liv, lys og luft i vores bygninger mellem byggeindustrien, byggeriets uddannelses- og forskningsinstitutioner i Danmark samt andre med interesse for liv, lys og luft.......LYSnET.com skal bidrage til at udbrede og udvikle viden om liv, lys og luft i vores bygninger mellem byggeindustrien, byggeriets uddannelses- og forskningsinstitutioner i Danmark samt andre med interesse for liv, lys og luft....

  5. Insight cognitif et schizophrenie

    OpenAIRE

    W. El-Hage; Lafay, N.; Wassouf, I.; Jaafari, N.

    2011-01-01

    Resume La schizophrenie est souvent associee a une meconnaissance du trouble severe et persistante. Ce deficit d?insight est correle a l?hypofrontalite mais independant du pronostic de la maladie ou du quotient intellectuel. L?insight cognitif est defini comme la difference entre la capacite de reflexion sur soi et la certitude dans cette reflexion. Cette capacite est trouvee diminuee dans la schizophrenie mais augmentee en cas de depression. Ainsi, la schizophrenie avec comorbidit...

  6. Caracterización de una permeasa de Rhizobium leguminosarum en el transporte de molibdeno y en la simbiosis con guisantes.

    OpenAIRE

    Alaminos Hervás, Elena

    2011-01-01

    Rhizobium leguminosarum es una bacteria que establece relaciones simbióticas con guisantes gracias a la fijación de nitrógeno en unas estructuras radiculares llamadas nódulos. La nitrogenasa es la enzima responsable de fijar nitrógeno y necesita molibdeno para su funcionamiento. Estudios recientes en otra bacteria, Rhodobacter capsulatus, han descubierto que existe una permeasa, PerO, implicada en el transporte de molibdeno al interior celular. En nuestro equipo de investigación disponemos ...

  7. Forestación de un terreno decapitado con Robinia pseudoacacia inoculada con Rhizobium spp. y Glomus deserticola Afforestation of a desurfaced field with Robinia pseudoacacia inoculated with Rhizobium spp. and Glomus deserticola

    Directory of Open Access Journals (Sweden)

    Alejandro E Ferrari

    2010-07-01

    Full Text Available Los terrenos decapitados constituyen un caso extremo de degradación de suelos aptos para agricultura, con graves consecuencias ambientales. El propósito de este trabajo fue ensayar los efectos de la inoculación de acacia blanca (Robinia pseudoacacia L. con una cepa efectiva de Rhizobium y un hongo de micorrizas arbusculares (Glomus deserticola en la supervivencia y crecimiento temprano de plantas trasplantadas a un terreno decapitado de Balcarce, comparando con fresno como árbol no fijador de nitrógeno. La supervivencia inicial fue muy buena (mayor del 77%, especialmente para los fresnos y las acacias inoculadas, las cuales mostraron significativamente mayor supervivencia al estrés por sequía que las acacias control. Todas las plantas se mostraron igualmente tolerantes al ataque de liebres y al estrés por heladas tardías. Durante el primer año las acacias inoculadas crecieron más rápido que las no inoculadas. En el segundo año las alturas se equipararon en parte, probablemente por la colonización de las acacias control con rizobios o micorrizas naturales del suelo. De todos modos, las acacias inoculadas siempre mostraron valores mayores de los distintos parámetros de desarrollo (altura total, diámetro de la copa y cantidad de ramificaciones que las plantas control, tanto en los sectores poco decapitados como en los más severamente degradados. En conclusión, la doble inoculación previa de acacia blanca mejoró la supervivencia inicial, aumentó tolerancia a la sequía y el crecimiento en 2 años y medio desde la plantación. Esta práctica de manejo sería entonces muy recomendable para reducir el período de vivero en la producción de árboles, así como lograr ejemplares más resistentes y mejor adaptados para proyectos de recuperación de suelos degradados.Many agricultural lands in Buenos Aires Province (Argentina have traditionally been desurfaced for the manufacture of bricks and other building materials. The desurfaced

  8. Entrepreneuriat Social et Participation Citoyenne

    Directory of Open Access Journals (Sweden)

    Sophie Larivet

    2010-01-01

    Full Text Available L'entrepreneuriat social est un concept émergeant, notamment dans les sciences de l'administration. Pourtant, en dehors d'une pratique directoriale spécifique, il est aussi une forme de participation citoyenne trop méconnue. L'objectif de cet article, basé sur une revue de la littérature et une approche théorique, est de présenter le concept d'entrepreneuriat social afin de mieux saisir son positionnement par rapport à la participation citoyenne. L'entrepreneuriat social constitue une forme particulière de participation à l'espace public par l'action, les entreprises sociales agissant au quotidien pour transformer le paysage social. En particulier, cet article souligne le contexte de développement de l'entrepreneuriat social, définit le concept et les notions connexes d'entreprise sociale et d'entrepreneur social, et, enfin, présente une réflexion sur la contribution de l'entrepreneuriat social à la participation citoyenne. L'article montre que l'entrepreneuriat social est une façon pour les citoyens d'agir directement et avec maîtrise sur la société. / Social entrepreneurship is an emerging concept, notably in administrative sciences. However, not only is it a specific managerial practice but it is also a type of citizen participation that is not well-known. The objective of this article, based on a literature review and a theoretical approach, is to present the concept of social entrepreneurship in order to better understand its relation to citizen participation. Social entrepreneurship represents a specific type of citizen participation involving actions. Social enterprises act daily to transform the social landscape. More specifically, this article presents the context of development of social entrepreneurship, proposes a definition of the concept and of other connected notions like "social enterprise" and "social entrepreneur", and, finally, analyzes the contribution of social entrepreneurship to citizen participation. It

  9. Greenhouse effect gases sources and sinks (CO{sub 2}, CH{sub 4}, N{sub 2}O) in grasslands and reduction strategies. Greenhouse effect gases prairies. Final report of the second part of the project. April 2004; Sources et puits de gaz a effet de serre (CO{sub 2}, CH{sub 4}, N{sub 2}O) en prairie paturee et strategies de reduction. GES-Prairies. Rapport final de la seconde tranche du projet. Avril 2004

    Energy Technology Data Exchange (ETDEWEB)

    Soussana, J.F

    2004-04-15

    The project 'GES-Prairies' (Greenhouse Gases - Grasslands) had two main objectives: 1. To measure more accurately the fluxes of CO{sub 2}, CH{sub 4} and N{sub 2}O of French grasslands and determine the greenhouse gas (GHG) balance of these areas. 2. To calculate the net GHG emissions of cattle production farms and finally to propose and evaluate some management scenarios leading to a reduction of GHG emissions. This project deals with three different spatial scales: the field scale, the farm scale and finally, the regional scale. At the field scale, during two years, fluxes of CO{sub 2}, CH{sub 4} and N{sub 2}O were measured in a mid-mountain permanent grassland, previously managed intensively by cutting and grazing (Laqueuille, Auvergne, France). Results from the first complete year of measurements show that the extensification process (reduction of the stocking rate and stopping N fertilization) allows to stock more carbon in the ecosystem. At the farm scale, We developed a model (FARMSIM, coupled to PASIM) able to simulate the GHG balance of a livestock farm. FARMSIM has been tested with data obtained from a mixed livestock farm in Lorraine (dairy and meat production, annual average stocking rate = 1.3 LU ha{sup -1}) of 100 ha (including 76 ha of grasslands and 21 of annual crops). The results indicate a net emission of 175 t equivalent C-CO{sub 2} for this farm. If expressed per unit of product, it represents 1.34 t equivalent C-CO{sub 2} per LU and per year or 0.54 kg CO{sub 2} per kg of milk and per year. At the regional scale/. The PASIM model has been used to simulate the European grasslands with a spatial resolution of 1' (about 200 * 200 km). For each grid cell, a sensibility analysis allowed to determine the N application which correspond to 30% of the N application that would maximize the annual yield of the pasture. Simulation runs on mixed systems (combining grazing and cutting) show that almost one half of the grassland area is, on

  10. A Rhizobium leguminosarum CHDL- (Cadherin-Like-) Lectin Participates in Assembly and Remodeling of the Biofilm Matrix

    Science.gov (United States)

    Vozza, Nicolás F.; Abdian, Patricia L.; Russo, Daniela M.; Mongiardini, Elías J.; Lodeiro, Aníbal R.; Molin, Søren; Zorreguieta, Angeles

    2016-01-01

    In natural environments most bacteria live in multicellular structures called biofilms. These cell aggregates are enclosed in a self-produced polymeric extracellular matrix, which protects the cells, provides mechanical stability and mediates cellular cohesion and adhesion to surfaces. Although important advances were made in the identification of the genetic and extracellular factors required for biofilm formation, the mechanisms leading to biofilm matrix assembly, and the roles of extracellular proteins in these processes are still poorly understood. The symbiont Rhizobium leguminosarum requires the synthesis of the acidic exopolysaccharide and the PrsDE secretion system to develop a mature biofilm. PrsDE is responsible for the secretion of the Rap family of proteins that share one or two Ra/CHDL (cadherin-like-) domains. RapA2 is a calcium-dependent lectin with a cadherin-like β sheet structure that specifically recognizes the exopolysaccharide, either as a capsular polysaccharide (CPS) or in its released form [extracellular polysaccharide (EPS)]. In this study, using gain and loss of function approaches combined with phenotypic and microscopic studies we demonstrated that RapA lectins are involved in biofilm matrix development and cellular cohesion. While the absence of any RapA protein increased the compactness of bacterial aggregates, high levels of RapA1 expanded distances between cells and favored the production of a dense matrix network. Whereas endogenous RapA(s) are predominantly located at one bacterial pole, we found that under overproduction conditions, RapA1 surrounded the cell in a way that was reminiscent of the capsule. Accordingly, polysaccharide analyses showed that the RapA lectins promote CPS formation at the expense of lower EPS production. Besides, polysaccharide analysis suggests that RapA modulates the EPS size profile. Collectively, these results show that the interaction of RapA lectins with the polysaccharide is involved in rhizobial

  11. PssP2 is a polysaccharide co-polymerase involved in exopolysaccharide chain-length determination in Rhizobium leguminosarum.

    Directory of Open Access Journals (Sweden)

    Małgorzata Marczak

    Full Text Available Production of extracellular polysaccharides is a complex process engaging proteins localized in different subcellular compartments, yet communicating with each other or even directly interacting in multicomponent complexes. Proteins involved in polymerization and transport of exopolysaccharide (EPS in Rhizobium leguminosarum are encoded within the chromosomal Pss-I cluster. However, genes implicated in polysaccharide synthesis are common in rhizobia, with several homologues of pss genes identified in other regions of the R. leguminosarum genome. One such region is chromosomally located Pss-II encoding proteins homologous to known components of the Wzx/Wzy-dependent polysaccharide synthesis and transport systems. The pssP2 gene encodes a protein similar to polysaccharide co-polymerases involved in determination of the length of polysaccharide chains in capsule and O-antigen biosynthesis. In this work, a mutant with a disrupted pssP2 gene was constructed and its capabilities to produce EPS and enter into a symbiotic relationship with clover were studied. The pssP2 mutant, while not altered in lipopolysaccharide (LPS, displayed changes in molecular mass distribution profile of EPS. Lack of the full-length PssP2 protein resulted in a reduction of high molecular weight EPS, yet polymerized to a longer length than in the RtTA1 wild type. The mutant strain was also more efficient in symbiotic performance. The functional interrelation between PssP2 and proteins encoded within the Pss-I region was further supported by data from bacterial two-hybrid assays providing evidence for PssP2 interactions with PssT polymerase, as well as glycosyltransferase PssC. A possible role for PssP2 in a complex involved in EPS chain-length determination is discussed.

  12. Nodulation of cowpeas and survival of cowpeas Rhizobia in acid, aluminum-rich soils. [Vigna unguiculata; Rhizobium

    Energy Technology Data Exchange (ETDEWEB)

    Hartel, P.G.; Whelan, A.M.; Alexander, M.

    1983-01-01

    A study was undertaken to determine whether the reduced nodulation of cowpeas (Vigna unguiculata (L.) Walp) grown in certain acid, Alrich soils resulted from the poor survival of the potentially infective rhizobia. Two strains of Rhizobium capable of nodulating cowpeas were used. The lowest pH for growth in defined liquid medium was 4.2 for one strain and 3.9 for the other. Only the latter was Al tolerant and could grow in a defined liquid medium containing 50 ..mu..M KAl(SO/sub 4/)/sub 2/. The survival of the bacteria and their ability to nodulate cowpeas in three soils were measured after the soils were amended with Ca or Al salts to give pH values ranging from 5.7 to 4.1 and extractable-Al concentrations from < 0.1 to 3.7 cmol(p/sup +/)/kg of soil. Only small differences in survival in 7 or 8 weeks were noted between the two strains. Plants inoculated with the Al-sensitive strain bore significantly fewer nodules in the more acid, Al-rich soils than in the same soils with higher pH values and less extractable Al. No significant reduction in nodule number was evident for plants inoculated with the Al-tolerant strain and grown in the more acid, Al-rich soils compared to cowpeas grown in the same soils with higher pH values and less extractable Al. It is suggested that the Al content of soil is not a major factor in the survival of cowpea rhizobia but that it does have a significant effect on nodulation. 24 references, 3 figures, 2 tables.

  13. Involvement of diamine oxidase and peroxidase in insolubilization of the extracellular matrix: implications for pea nodule initiation by Rhizobium leguminosarum.

    Science.gov (United States)

    Wisniewski, J P; Rathbun, E A; Knox, J P; Brewin, N J

    2000-04-01

    Rhizobium leguminosarum colonizes host cells and tissues through infection threads, which are tubular in-growths of the plant cell wall. Monoclonal antibody MAC265 recognizes a plant matrix glycoprotein (MGP) associated with the lumen of these infection threads. This glycoprotein is also released in soluble form from the root tips of pea seedlings. In the presence of hydrogen peroxide, release of glycoprotein from root tips was not observed. Extractability from root tips was therefore used as the basis for investigating the peroxide-driven insolubilization of MGP and the possible involvement of two extracellular enzymes, peroxidase (POD) and diamine oxidase (DAO), was investigated. Release of MGP from root tips was enhanced by application of POD and DAO inhibitors (salicylhydroxamic acid and o-phenanthroline, respectively). Furthermore, release of MGP was inhibited by pretreatment of roots with putrescine (the substrate of DAO) and also by application of a partially purified extract of DAO from pea shoots. Following inoculation of pea roots with R. leguminosarum, elevated levels of DAO transcript were observed by reverse transcriptase-polymerase chain reaction (RT-PCR), but these then dropped to a low level from 4 to 10 days post inoculation, rising again in more mature nodules. In situ hybridization studies indicated that the bulk of the transcription was associated with the infected tissue in the center of the nodule. On the basis of these observations, we postulate that DAO may be involved in the peroxide-driven hardening of MGP in the lumen of infection threads and in the intercellular matrix.

  14. Microcystin-tolerant Rhizobium protects plants and improves nitrogen assimilation in Vicia faba irrigated with microcystin-containing waters.

    Science.gov (United States)

    Lahrouni, Majida; Oufdou, Khalid; El Khalloufi, Fatima; Benidire, Loubna; Albert, Susann; Göttfert, Michael; Caviedes, Miguel A; Rodriguez-Llorente, Ignacio D; Oudra, Brahim; Pajuelo, Eloísa

    2016-05-01

    Irrigation of crops with microcystins (MCs)-containing waters-due to cyanobacterial blooms-affects plant productivity and could be a way for these potent toxins entering the food chain. This study was performed to establish whether MC-tolerant rhizobia could benefit growth, nodulation, and nitrogen metabolism of faba bean plants irrigated with MC-containing waters. For that, three different rhizobial strains-with different sensitivity toward MCs-were used: RhOF96 (most MC-sensitive strain), RhOF125 (most MC-tolerant strain), or Vicz1.1 (reference strain). As a control, plants grown without rhizobia and fertilized by NH4NO3 were included in the study. MC exposure decreased roots (30-37 %) and shoots (up to 15 %) dry weights in un-inoculated plants, whereas inoculation with rhizobia protects plants toward the toxic effects of MCs. Nodulation and nitrogen content were significantly impaired by MCs, with the exception of plants inoculated with the most tolerant strain RhOF125. In order to deep into the effect of inoculation on nitrogen metabolism, the nitrogen assimilatory enzymes (glutamine synthetase (GS) and glutamate synthase (GOGAT)) were investigated: Fertilized plants showed decreased levels (15-30 %) of these enzymes, both in shoots and roots. By contrast, inoculated plants retained the levels of these enzymes in shoots and roots, as well as the levels of NADH-GOGAT activity in nodules. We conclude that the microcystin-tolerant Rhizobium protects faba bean plants and improves nitrogen assimilation when grown in the presence of MCs. PMID:26865488

  15. Interacción de Tsukamurella paurometabola C-924 con Rhizobium leguminosarum biovar phaseoli CFH en el cultivo de frijol

    Directory of Open Access Journals (Sweden)

    Marieta Marín Bruzos

    2013-01-01

    Full Text Available En el estudio se evaluaron, mediante análisis de los parámetros fenológicos de las plantas, la interacción de Tsukamurella paurometabola C-924 con Rhizobium leguminosarum biovar phaseoli CFH en el cultivo de frijol. Se emplearon cuatro tratamientos: plantas sin inocular (control, inoculadas con T. paurometabola C-924, inoculadas con R. leguminosarum biovar phaseoli CFH e inoculadas con ambas cepas. Se observaron diferencias significativas (P < 0.01 en los porcentajes de germinación de las plantas tratadas con microorganismos de forma independiente o conjunta con respecto al control sin inocular. Se determinó que la inoculación de T. paurometabola C-924 afectó el proceso de nodulación de R. leguminosarum biovar phaseoli CFH. Sin embargo, esto no incidió de manera significativa en la altura de las plantas ni en el diámetro del tallo, ya que no se encontraron diferencias entre los tratamientos para estos parámetros. Para el número de hojas, los mejores resultados se obtuvieron con la aplicación de T. paurometabola C-924. Se concluyó que la interacción de T. paurometabola C-924 con R. leguminosarum biovar phaseoli CFH en el frijol estimuló significativamente la germinación de las semillas y el número de hojas de las plantas con respecto al control sin inocular. Aunque la aplicación de T. paurometabola C-924 no favoreció la nodulación de R. leguminosarum biovar phaseoli CFH, esto no afectó las características fenológicas del cultivo.

  16. Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environment

    Directory of Open Access Journals (Sweden)

    Piersiak Tomasz

    2010-11-01

    Full Text Available Abstract Background Rhizobium leguminosarum bv. trifolii is a symbiotic nitrogen-fixing bacterium that elicits nodules on roots of host plants Trifolium spp. Bacterial surface polysaccharides are crucial for establishment of a successful symbiosis with legumes that form indeterminate-type nodules, such as Trifolium, Pisum, Vicia, and Medicago spp. and aid the bacterium in withstanding osmotic and other environmental stresses. Recently, the R. leguminosarum bv. trifolii RosR regulatory protein which controls exopolysaccharide production has been identified and characterized. Results In this work, we extend our earlier studies to the characterization of rosR mutants which exhibit pleiotropic phenotypes. The mutants produce three times less exopolysaccharide than the wild type, and the low-molecular-weight fraction in that polymer is greatly reduced. Mutation in rosR also results in quantitative alterations in the polysaccharide constituent of lipopolysaccharide. The rosR mutants are more sensitive to surface-active detergents, antibiotics of the beta-lactam group and some osmolytes, indicating changes in the bacterial membranes. In addition, the rosR mutants exhibit significant decrease in motility and form a biofilm on plastic surfaces, which differs significantly in depth, architecture, and bacterial viability from that of the wild type. The most striking effect of rosR mutation is the considerably decreased attachment and colonization of root hairs, indicating that the mutation affects the first stage of the invasion process. Infection threads initiate at a drastically reduced rate and frequently abort before they reach the base of root hairs. Although these mutants form nodules on clover, they are unable to fix nitrogen and are outcompeted by the wild type in mixed inoculations, demonstrating that functional rosR is important for competitive nodulation. Conclusions This report demonstrates the significant role RosR regulatory protein plays in

  17. Rhizobium leguminosarum bv. trifolii rosR is required for interaction with clover, biofilm formation and adaptation to the environment

    Science.gov (United States)

    2010-01-01

    Background Rhizobium leguminosarum bv. trifolii is a symbiotic nitrogen-fixing bacterium that elicits nodules on roots of host plants Trifolium spp. Bacterial surface polysaccharides are crucial for establishment of a successful symbiosis with legumes that form indeterminate-type nodules, such as Trifolium, Pisum, Vicia, and Medicago spp. and aid the bacterium in withstanding osmotic and other environmental stresses. Recently, the R. leguminosarum bv. trifolii RosR regulatory protein which controls exopolysaccharide production has been identified and characterized. Results In this work, we extend our earlier studies to the characterization of rosR mutants which exhibit pleiotropic phenotypes. The mutants produce three times less exopolysaccharide than the wild type, and the low-molecular-weight fraction in that polymer is greatly reduced. Mutation in rosR also results in quantitative alterations in the polysaccharide constituent of lipopolysaccharide. The rosR mutants are more sensitive to surface-active detergents, antibiotics of the beta-lactam group and some osmolytes, indicating changes in the bacterial membranes. In addition, the rosR mutants exhibit significant decrease in motility and form a biofilm on plastic surfaces, which differs significantly in depth, architecture, and bacterial viability from that of the wild type. The most striking effect of rosR mutation is the considerably decreased attachment and colonization of root hairs, indicating that the mutation affects the first stage of the invasion process. Infection threads initiate at a drastically reduced rate and frequently abort before they reach the base of root hairs. Although these mutants form nodules on clover, they are unable to fix nitrogen and are outcompeted by the wild type in mixed inoculations, demonstrating that functional rosR is important for competitive nodulation. Conclusions This report demonstrates the significant role RosR regulatory protein plays in bacterial stress adaptation

  18. Co-inoculation of Rhizobium tropici and Azospirillum brasilense in common beans grown under two irrigation depths

    Directory of Open Access Journals (Sweden)

    Amanda Ribeiro Peres²*

    2016-04-01

    Full Text Available ABSTRACT The alternative technique of co-inoculation or mixed inoculation with symbiotic and non-symbiotic bacteria has been studied in leguminous plants. However, there are few field studies with common beans and under the influence of the amount of irrigated water. Thus, the objective of this study was to evaluate the efficiency of inoculation and co-inoculation of common beans with Rhizobium tropici and Azospirillum brasilense under two irrigation depths. The experiment was carried out in the winter of 2012 and 2013, in Selvíria, state of Mato Grosso do Sul. The experimental design was composed of randomized blocks in split-plot scheme with two irrigation depths in the plots (recommended for common beans and 75% of the recommended and five forms of nitrogen (N supply in the split-plots (control non-inoculated with 40 kg ha- 1 of N in topdressing, 80 kg ha- 1 of N in topdressing, A. brasilense inoculation with 40 kg ha-1 of N in topdressing, R. tropici inoculation with 40 kg ha-1 of N in topdressing, and co-inoculation of A. brasilense and R. tropici with 40 kg ha- 1 of N in topdressing with four repetitions. Co-inoculation increased nodulation in the second year of cultivation. None of the evaluated treatments increased the grain yield in relation to non-inoculated control with 40 kg ha-1 of nitrogen in topdressing, which presented average yield of 2,200 kg ha-1. The use of 75% of the recommended irrigation depth provides similar grain yield to the recommended irrigation depth in common beans cropped in winter.

  19. Compatibility of rhizobial genotypes within natural populations of Rhizobium leguminosarum biovar viciae for nodulation of host legumes.

    Science.gov (United States)

    Laguerre, Gisèle; Louvrier, Philippe; Allard, Marie-Reine; Amarger, Noëlle

    2003-04-01

    Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean (Vicia faba) and pea (Pisum sativum) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches (Vicia sativa) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia were characterized by repetitive extragenomic palindromic-PCR fingerprinting and/or PCR-restriction fragment length polymorphism (RFLP) of 16S-23S ribosomal DNA intergenic spacers as markers of the genomic background and PCR-RFLP of a nodulation gene region, nodD, as a marker of the symbiotic component of the genome. Pairwise comparisons showed differences among the genetic structures of the bulk soil, rhizosphere, and nodule populations and in the degree of host specificity within the Vicieae cross-inoculation group. With fava bean, the symbiotic genotype appeared to be the preponderant determinant of the success in nodule occupancy of rhizobial genotypes independently of the associated genomic background, the plant genotype, and the soil sampled. The interaction between one particular rhizobial symbiotic genotype and fava bean seems to be highly specific for nodulation and linked to the efficiency of nitrogen fixation. By contrast with bulk soil and fava bean-nodulating populations, the analysis of pea-nodulating populations showed preferential associations between genomic backgrounds and symbiotic genotypes. Both components of the rhizobial genome may influence competitiveness for nodulation of pea, and rhizosphere colonization may be a decisive step in competition for nodule occupancy. PMID:12676710

  20. Dual role of HupF in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum

    Directory of Open Access Journals (Sweden)

    Albareda Marta

    2012-11-01

    Full Text Available Abstract Background [NiFe] hydrogenases are enzymes that catalyze the oxidation of hydrogen into protons and electrons, to use H2 as energy source, or the production of hydrogen through proton reduction, as an escape valve for the excess of reduction equivalents in anaerobic metabolism. Biosynthesis of [NiFe] hydrogenases is a complex process that occurs in the cytoplasm, where a number of auxiliary proteins are required to synthesize and insert the metal cofactors into the enzyme structural units. The endosymbiotic bacterium Rhizobium leguminosarum requires the products of eighteen genes (hupSLCDEFGHIJKhypABFCDEX to synthesize an active hydrogenase. hupF and hupK genes are found only in hydrogenase clusters from bacteria expressing hydrogenase in the presence of oxygen. Results HupF is a HypC paralogue with a similar predicted structure, except for the C-terminal domain present only in HupF. Deletion of hupF results in the inability to process the hydrogenase large subunit HupL, and also in reduced stability of this subunit when cells are exposed to high oxygen tensions. A ΔhupF mutant was fully complemented for hydrogenase activity by a C-terminal deletion derivative under symbiotic, ultra low-oxygen tensions, but only partial complementation was observed in free living cells under higher oxygen tensions (1% or 3%. Co-purification experiments using StrepTag-labelled HupF derivatives and mass spectrometry analysis indicate the existence of a major complex involving HupL and HupF, and a less abundant HupF-HupK complex. Conclusions The results indicate that HupF has a dual role during hydrogenase biosynthesis: it is required for hydrogenase large subunit processing and it also acts as a chaperone to stabilize HupL when hydrogenase is synthesized in the presence of oxygen.

  1. Non-pathogenic Rhizobium radiobacter F4 deploys plant beneficial activity independent of its host Piriformospora indica.

    Science.gov (United States)

    Glaeser, Stefanie P; Imani, Jafargholi; Alabid, Ibrahim; Guo, Huijuan; Kumar, Neelendra; Kämpfer, Peter; Hardt, Martin; Blom, Jochen; Goesmann, Alexander; Rothballer, Michael; Hartmann, Anton; Kogel, Karl-Heinz

    2016-04-01

    The Alphaproteobacterium Rhizobium radiobacter F4 (RrF4) was originally characterized as an endofungal bacterium in the beneficial endophytic Sebacinalean fungus Piriformospora indica. Although attempts to cure P. indica from RrF4 repeatedly failed, the bacterium can easily be grown in pure culture. Here, we report on RrF4's genome and the beneficial impact the free-living bacterium has on plants. In contrast to other endofungal bacteria, the genome size of RrF4 is not reduced. Instead, it shows a high degree of similarity to the plant pathogenic R. radiobacter (formerly: Agrobacterium tumefaciens) C58, except vibrant differences in both the tumor-inducing (pTi) and the accessor (pAt) plasmids, which can explain the loss of RrF4's pathogenicity. Similar to its fungal host, RrF4 colonizes plant roots without host preference and forms aggregates of attached cells and dense biofilms at the root surface of maturation zones. RrF4-colonized plants show increased biomass and enhanced resistance against bacterial leaf pathogens. Mutational analysis showed that, similar to P. indica, resistance mediated by RrF4 was dependent on the plant's jasmonate-based induced systemic resistance (ISR) pathway. Consistent with this, RrF4- and P. indica-induced pattern of defense gene expression were similar. In clear contrast to P. indica, but similar to plant growth-promoting rhizobacteria, RrF4 colonized not only the root outer cortex but also spread beyond the endodermis into the stele. On the basis of our findings, RrF4 is an efficient plant growth-promoting bacterium. PMID:26495996

  2. ETS fusion genes in prostate cancer.

    Science.gov (United States)

    Gasi Tandefelt, Delila; Boormans, Joost; Hermans, Karin; Trapman, Jan

    2014-06-01

    Prostate cancer is very common in elderly men in developed countries. Unravelling the molecular and biological processes that contribute to tumor development and progressive growth, including its heterogeneity, is a challenging task. The fusion of the genes ERG and TMPRSS2 is the most frequent genomic alteration in prostate cancer. ERG is an oncogene that encodes a member of the family of ETS transcription factors. At lower frequency, other members of this gene family are also rearranged and overexpressed in prostate cancer. TMPRSS2 is an androgen-regulated gene that is preferentially expressed in the prostate. Most of the less frequent ETS fusion partners are also androgen-regulated and prostate-specific. During the last few years, novel concepts of the process of gene fusion have emerged, and initial experimental results explaining the function of the ETS genes ERG and ETV1 in prostate cancer have been published. In this review, we focus on the most relevant ETS gene fusions and summarize the current knowledge of the role of ETS transcription factors in prostate cancer. Finally, we discuss the clinical relevance of TMRPSS2-ERG and other ETS gene fusions in prostate cancer.

  3. Final report of the project. Emission of nitrogen oxides by the soils. Measures, modelization, land registry and inventory. Impact on the air quality, the climatic change and the evaluation of possibilities of these emissions reduction; Rapport final du projet. Emissions d'oxydes d'azote par les sols. Mesures, modelisation, cadastre et inventaire. Impact sur la qualite de l'air, le changement climatique, et evaluation des gisements de reduction de ces emissions

    Energy Technology Data Exchange (ETDEWEB)

    Serca, D.; Cortinovis, J. [LA Laboratoire d' Aerologie UMR 5560, 31 - Toulouse (France); Laville, P.; Gabrielle, B. [Institut National de Recherches Agronomiques (INRA), Environnement et Grandes Cultures, 78 - Thiverval-Grignon (France); Beekmann, M.; Ravetta, F. [Universite Pierre et Marie Curie, SA Service d' Aeronomie, 75 - Paris (France); Henault, C. [Institut National de Recherches Agronomiques (INRA-LMS) UMR Microbiologie des Sols-Geosol, 21 - Dijon (France)

    2007-07-01

    This project deals with NOx biosphere-atmosphere exchanges, NOx being considered as an indirect greenhouse gases (tropospheric O{sub 3} precursor). It relies on four laboratory specialized both on the soil-plant-atmosphere interface, and on the atmospheric chemistry. Methodology used bear on a set of in situ and laboratory measurements aiming at improving existing emission parameterization, or building new ones for the agro-ecosystems encountered in France or Europe. In situ measurements allowed to study the emission phenology in relation with relevant environmental parameters (meteorological, soil characteristics, and agricultural). Laboratory measurements allowed to establish an emission algorithm related to the three main parameters, that is, soil temperature, water and ammonia content. This algorithm has been adapted and simplified to spatialize the emissions at the France level. This spatialization was performed using environmental parameters accessible through data base (ECMWF) or agricultural statistics (such as nitrogen inputs, land use, crops). Spatial and temporal extrapolation allowed reaching the main objective, that is, to build a national inventory for a reference year (2002). This inventory allowed determining the contribution of NOx emitted by soil as compared to total emitted NOx, and the proportion of NOx emitted by soil due to fertilizer use. Our study, based on 57% of the French used agricultural area, and extrapolated to the whole arable surface, shows that soils would be responsible of about 5% of the total NOx emissions. On these 5%, 20%, which finally is a rather low percentage, would be linked to fertilizer use. The impact of these emissions on the atmospheric chemistry has been evaluated using the CHIMERE chemistry-transport model. We found that NOx emissions from soil would be of minor importance when compared to the industrial emissions, being a factor of ten lower in France. As a matter of consequence, the impact of the emissions on the

  4. Enfants, chercheurs et citoyens

    CERN Document Server

    Lederman, Leon

    1998-01-01

    J'ai consacré ma vie à la physique. J'aimerais que tous les enfants aient la chance de satisfaire leur goût pour la recherche et de développer leur intelligence. J'ai découvert à Chicago, chez mon ami Leon Lederman, une méthode d'enseignement des sciences à l'école qui m'a subjugué. J'ai vu, à tous les niveaux, en physique, en chimie, en sciences naturelles et en mathématiques, des enfants qui expérimentaient avec joie, apprenaient les concepts fondamentaux à leur rythme, réfléchissant et discutant. Je voyais en gestation des êtres libres, capables de rechercher une vérité qui ne leur était pas assenée. J'ai voulu confronter des enseignants français du primaire et du secondaire à ces recherches qui ne se limitent pas à l'enseignement primaire. C'est leur regard critique et surtout leurs idées pour notre enseignement que j'ai rassemblés dans ce livre.

  5. La balle et la plume

    Directory of Open Access Journals (Sweden)

    Peter Marquis

    2012-06-01

    Full Text Available Fin octobre 2008, la nouvelle fit l’effet d’une bombe : Barack Obama, alors candidat à la présidence américaine, demanda à la chaîne câblée Fox News de retarder le début du sixième match de la World Series de base-ball afin de diffuser un spot de campagne. Son adversaire, John McCain, soutenu par le camp républicain et une partie de la population, monta aussitôt au créneau pour dénoncer l’hérésie : s’arroger le droit de perturber cette grande finale du championnat au déroulement immuable reve...

  6. CARDIOPATHIE ET GROSSESSE

    OpenAIRE

    BENHAMMOU, Meryem; HAMZAOUI, Souleymane; ALLAOUI, Imane; BETAOUAF, Meryem; LAHCENE, Aicha; CHAALA, Zeynab

    2015-01-01

    La grossesse chez la femme enceinte porteuse d'une valvulopathie ou d’une autre cardiopathie est associée à un risque très variable selon la nature et la sévérité de celle-ci. Ce risque dépend également de la qualité de la prise en charge. Une collaboration pluridisciplinaire efficace conjuguée aux progrès des traitements et des outils de surveillance permettrait de réduire ce risque et d’améliorer le pronostic materno-foetal. Ainsi, toutes les patientes atteintes d’une valvulopathie néces...

  7. Seconde Intifada et terrorisme

    OpenAIRE

    Garcin-Marrou, Isabelle; Tétu, Jean-François

    2003-01-01

    19 septembre 2002. Après environ un mois et demie d'accalmie (et la réoccupationquasi générale de la Cisjordanie par Tsahal), une attaque-suicide fait 5 morts à TelAviv. Le lendemain, soit environ deux ans après le début de la seconde Intifada,l'armée israélienne assiège de nouveau le QG de Yasser Arafat à Ramallah, auxtrois quarts détruit, et le gouvernement d'Israël, comme au printemps précédent,tente de le pousser à l'exil. Moins de 15 jours plus tôt, à peu près un an après lesattentats du...

  8. Contextualisation : stylistique et statistique

    OpenAIRE

    Magri-Mourgues, Véronique

    2012-01-01

    La dimension du contexte des cooccurrences varie depuis le premier palier, celui du syntagme et de la phrase, à celui du corpus, observatoire construit par le chercheur, en passant par l’unité conventionnelle du paragraphe et l’unité textuelle déterminée par des propriétés de cohésion. La contextualisation permet d’établir les bases d’une stylistique différentielle où les opérations interprétatives de rapprochement et de différenciation s’exercent par rapport à un contexte préalablement défin...

  9. Experimental Study of the Rhizobium and Iron Oxide Thiobacillus for Heap Leaching of Zinc Leaching Slag%根瘤菌与氧化铁硫杆菌堆浸锌浸出渣的试验研究

    Institute of Scientific and Technical Information of China (English)

    李勇; 邓兴; 金开胜; 杨光棣

    2012-01-01

    This paper adopts the rhizobium and thiobaeillus ferrooxidans respectively for heap leaching processing of hydrometallurgy of zinc leaching residue. Through the treatment of rhizobium with thiobacillus ferrooxidans, zinc leaching residue of zinc leaching rate is compared. The test results indicate that rhizobium treatment of zinc leaching residue of zinc leaching rate reaches 24.12 % and thiobacillus ferrooxidans treatment of zinc leaching residue of zinc leaching rate reaches 33.86 %. Treatment rate by thiobacillus ferrooxidans was significantly higher than that of rhizobium.%本文采用根瘤菌与氧化铁硫杆菌分别堆浸处理湿法冶金的锌浸出渣,通过比较根瘤菌与氧化铁硫杆菌处理的锌浸出渣的锌浸出率。试验结果表明,根瘤菌处理锌浸出渣锌浸出率达到了24.12%,而氧化铁硫杆菌处理的锌浸出渣锌浸出率达到了33.86%,氧化铁硫杆菌处理锌浸出渣的锌浸出率明显高于根瘤菌。

  10. PERANAN FUNGI MIKORIZA ARBUSKULA, MIKROORGANISME PELARUT FOSFAT, RHIZOBIUM SP DAN ASAM HUMIK UNTUK MENINGKATKAN PERTUMBUHAN DAN PRODUKTIVITAS LEGUM Calopogonium mucunoides PADA TANAH LATOSOL DAN TAILING TAMBANG EMAS DI PT. ANEKA TAMBANG

    Directory of Open Access Journals (Sweden)

    - Karti

    2014-06-01

    Full Text Available Latosols soil conditions are poor in nutrients and tailing of gold mine contain heavy metal causes low productivity of forage. The objective of this research to study the best combination of soil potential microorganisms and soil conditioner that can promote the growth and production of legume crops Calopogonium mucunoides Desv. Seven treatments used were A: control, B: arbuscular mycorrhizal fungi (AMF, C: AMF + Rhizobium, D: AMF + Phosphate Solubilizing Bacteria (BPF, E: AMF + Humic Acid, F: AMF + BPF + Rhizobium, G: AMF + BPF + Humic Acid + Rhizobium. Variables measured were the root dry weight, shoot dry weight, spread length, number of trifoliate leaves, the number of active root nodules, the percentage of root infection. Augmentation of soil microorganisms and soil conditioner not effective enough to improve plant growth of Calopogonium mucunoides Desv because plants can still adapt and grow well on latosols. Plants grown on tailings provide the best response is G (AMF+ Humic Acid + Rhizobium, although the rate of growth is not as good as plants grown in latosols.

  11. Characterization of a symbiotically effective Rhizobium resistant to arsenic: Isolated from the root nodules of Vigna mungo (L.) Hepper grown in an arsenic-contaminated field.

    Science.gov (United States)

    Mandal, Santi M; Pati, Bikas R; Das, Amit K; Ghosh, Ananta K

    2008-04-01

    Bacteria were isolated from the root nodules of Vigna mungo (L.) Hepper, grown in an arsenic-contaminated field and the strain was selected by its nodulation ability as well as better arsenic tolerant capacity compared to others. The selected strain was identified as Rhizobium by 16S rDNA sequencing and designated as VMA301. Phylogenetic analysis of the gene sequences showed its close relatedness with Sinorhizobium fredii. LC(50) value of arsenate for the bacteria as determined by flow cytometry was found to be 2.8 mM and arsenic uptake was measured by atomic absorption spectrometry as 0.048 mg g(-1) biomass. The high amount of arsenic was toxic to the cell, which changed the morphology of the bacteria to an elongated shape. Presence of a transcriptional regulatory gene (ArsR) of the ars genetic system was confirmed by amplification and sequencing. The symbiotic property of the isolate was also confirmed by amplification and sequencing of the NodC gene. These results indicate that the isolated Rhizobium bacteria may exert dual roles in the environment, arsenic bioremediation from the soil as well as increase of soil fertility through nitrogen fixation.

  12. Reduction in pathogen populations at grapevine wound sites is associated with the mechanism underlying the biological control of crown gall by rhizobium vitis strain ARK-1.

    Science.gov (United States)

    Kawaguchi, Akira

    2014-09-17

    A nonpathogenic strain of Rhizobium (=Agrobacterium) vitis, ARK-1, limited the development of grapevine crown gall. A co-inoculation with ARK-1 and the tumorigenic strain VAT07-1 at a 1:1 cell ratio resulted in a higher population of ARK-1 than VAT07-1 in shoots without tumors, but a significantly lower population of ARK-1 than VAT07-1 in grapevine shoots with tumors. ARK-1 began to significantly suppress the VAT07-1 population 2 d after the inoculation. This result indicated that ARK-1 reduced the pathogen population at the wound site through biological control. Although ARK-1 produced a zone of inhibition against other tumorigenic Rhizobium spp. in in vitro assays, antibiosis depended on the culture medium. ARK-1 did not inhibit the growth of tumorigenic R. radiobacter strain AtC1 in the antibiosis assay, but suppressed the AtC1-induced formation of tumors on grapevine shoots, suggesting that antibiosis by ARK-1 may not be the main mechanism responsible for biological control.

  13. Studies on the Alfalfa Growing Effects of Alfalfa Rhizobium%苜蓿根瘤菌剂对紫花苜蓿生长影响研究

    Institute of Scientific and Technical Information of China (English)

    樊川; 杨旭升; 曹亚斌

    2015-01-01

    通过不同剂量苜蓿根瘤菌接种两种紫花苜蓿试验,比较了接种根瘤菌剂对紫花苜蓿生长的影响。试验结果表明:接种苜蓿根瘤菌剂能够提高紫花苜蓿结瘤数量和根瘤重,促进紫花苜蓿的生长发育,增加苜蓿草的产草量。在所试验的接种剂量中,以每 kg 苜蓿种子接种12mL 根瘤菌剂为最佳接种量。%This paper studied the alfalfa growing effects of alfalfa rhizobium by inoculated different amounts.The experimental results showed that inoculating rhizobium can raise the nodulating amount of alfalfa and the weight of nodulations,promoting the growth of alfalfas and augment the productions of alfalfas.Among the different inoculated amounts,the amount of 12ml is the best inoculated amount.

  14. Effect of aldicarb on growth and radio-carbon (14C) and radio-phosphorus (32P) assimilation by Rhizobium japonicum

    International Nuclear Information System (INIS)

    In vitro studies on the effect of aldicarb (2-methyl-2-(methyl thio) propionaldehde-o-methyl carbamoyl oxime), a soil applied systemic insecticide, on Rhizobium japonicum revealed that the chemical (at 1,2 and 5 ppm levels) stimulated the growth of the organism initially upto 48 hr which declined thereafter upto 72 hr. The incorporation of 14C-glucose by the cells considerably reduced due to the insecticide treatment. The production of extracellular, water-soluble slime (polysacchardes) was also reduced considerably with increased concentrations of the chemical. However, the incorporation of 14C-radio-activity in the extracellular slime generally enhanced due to the treatment, upto 6 hr after injection of the radioactivity, which declined significantly later at 15 hr, indicating a qualitative difference in the extracellular polysaccharides produced by the insecticide treated cells. The insecticide treatment drastically reduced the incorporation of 32P-disodium hydrogen phosphate into Rhizobium cells, but enhanced the specific activity of the extracellular polysacchrides. (author)

  15. Comportement vieillissement et peau

    OpenAIRE

    Jacquet, Emmanuelle; Chambert, Jérôme; Remache, Djamel

    2013-01-01

    National audience Il apparaît souvent saugrenu aux mécaniciens que l'un des leurs s'intéresse au tégument humain. Pourtant, la connaissance du comportement et des réponses de ce tissu aux sollicitations extérieures est essentielle dans beaucoup de domaines et pas seulement celui des dermatologues qui à eux seuls constituent déjà un champ large d'intérêt pour développer, par exemple, des tissus de substitution aux propriétés voisines de celles du tissu cutané. Parmi ces domaines, je citerai...

  16. Retard Pubertaire et Hypogonadisme

    OpenAIRE

    VALDES SOCIN, Hernan Gonzalo

    2015-01-01

    Puberté et Hypogonadisme Hypogonadotrope Prof AS Parent & Dr H Valdes-Socin. Le retard pubertaire est une des manifestations cliniques les plus significatives de l’hypogonadisme hypogonadotrope, et un véritable défi diagnostic pour l’ensemble des cliniciens. Il faut cependant rappeler que l'activité de l'axe hypothalamo-hypohyso-gonadique (HHG) se déroule en plusieurs phases. A leur tour, ces différentes étapes sont une fenêtre diagnostique privilégiée pour suspecter un hypogonadis...

  17. Cyberespace et cyberculture

    OpenAIRE

    Pierre Lévy

    1999-01-01

    Alors, pendant la petite heure que va durer cette conférence, je voudrais partager avec vous quelques réflexions et quelques hypothèses sur la Cyberculture. Je voudrais d'abord situer les choses, mon propos est un propos de philosophe, autrement dit, il va s'agir de spéculations sur des concepts. Donc, je vous avertis tout de suite à quelle sauce vous allez être mangés. Il ne s'agit pas d'un discours culturel critique, comme les intellectuels français en ont l'habitude, et il ne s'agit pas no...

  18. Mafia et comportements mafieux

    OpenAIRE

    Ghiloni, Jacques

    2011-01-01

    Dans le film Gomorra , consa-cré à la mafia napolitaine, on pouvait voir les rites initiatiques, la structuration hiérarchique et familiale du sous-prolétariat mafieux. On découvrait également les rapports d'intérêts qui reliaient la Camorra et l'économie capita-liste, au travers de l'enfouissement illégal des déchets industriels européens. Nous retrouvons dans ce numéro de la revue Illusio ces rites initiatiques, cette logique de domination, cette structuration familiale. Mais de manière plu...

  19. Stylistique et discours scientifique

    OpenAIRE

    Gilles MATHIS

    2012-01-01

    Cette communication, après avoir présenté la pluralité des pratiques de la stylistique (étude de style, analyse de style, analyse rhétorique) en liaison avec les conceptions du style et dégagé les éléments fondamentaux de la caractérisation du discours scientifique, pose les conditions épistémologiques et méthodologiques d'une application spécifique de la stylistique à l'étude du discours scientifique. Elle en illustre la pratique par quelques exemples en annexe.

  20. Hvad karakteriserer et talent?

    DEFF Research Database (Denmark)

    Raalskov, Jesper

    2012-01-01

    Debatten i medierne har i stigende grad fokus på talentfulde medarbejdere - men hvad er talent egentlig? I den akademiske litteratur inden for Talent Management (TM) gives der mange forskellige bud på, hvordan man som virksomhed kan udvikle og lede talenter, men færre af feltets inputgivere...... forholder sig til, hvad den overordnede definition af talent er. Der ses i litteraturen divergerende opfattelser af, hvilke konkrete egenskaber (attributter) som definerer et talent. I denne artikel gives et bud på en konsensusskabende tilgang til talent, idet talent kan ses som; det du har gjort (fortid...

  1. Aspects Technologiques et Developpementaspects Technologiques et Developpement

    Directory of Open Access Journals (Sweden)

    Sebedio Jean-Louis

    2001-07-01

    Full Text Available L’acide linoléique conjugué (CLA est présent dans les aliments d’origine animale, en particulier ceux issus des ruminants. Le CLA peut aussi être synthétisé au laboratoire par isomérisation alcaline de l’acide linoléique. Alors que l’isomère naturel est le 18 : 2 9c,11t, les produits de synthèse contiennent principalement deux isomères, les 18 : 2 9c,11t et 10t,12c. De nombreuses études menées sur des modèles animaux, le plus souvent avec des mélanges synthétiques, montrent des effets potentiellement bénéfiques pour la santé (anticarcinogènes, protecteurs vis-à-vis de l’athérosclérose, modulateurs de la composition corporelle… alors que la plupart des études menées chez l’homme concernent les effets des CLA sur la composition corporelle. À ce jour, les données concernant les effets sur la composition corporelle chez l’homme semblent indiquer un bénéfice potentiel, mais les résultats restent controversés. Par ailleurs, bien qu’un effet anticarcinogène soit démontré chez l’animal, aucune étude chez l’homme n’a pu établir de relation entre CLA et cancer du sein. De plus, peu d’études traitant des aspects sécuritaires liés à la consommation à long terme ont été publiées. Par conséquent, il est nécessaire de disposer de plus de données en particulier chez l’Homme pour garantir l’innocuité de ces acides gras afin de protéger le consommateur, avant d’envisager l’utilisation de CLA dans des aliments fonctionnels.

  2. Grorum. Et demonstrationsprojekt

    DEFF Research Database (Denmark)

    Traberg-Borup, Steen

    Meddelelsen beskriver udforming og indretning af et grorum opført af fabriksfremstillede komponenter. Alle klimaskærmens dele, dvs. gulv, vægge, loft og dør gennemgås med udførlige bygningstegninger. Desuden beskrives ventilationsanlægget, belysningsanlægget og det specielt udviklede reolsystem....

  3. Ledelse i et interventionsperspektiv

    DEFF Research Database (Denmark)

    Keiding, Tina Bering

    2012-01-01

    at etablere et teoretisk fundament for de praktiske anvisninger end på at formulere slagkraftige ledelsesslogans. Ethvert ledelsessystem og enhver leder til hver en tid må udvælge sine konkrete ledelsestiltag og realisere disse på sin egen unikke måde, og jeg finder det derfor afgørende, at kapitlet ikke blot...

  4. Theories and calculation methods for regional objective ET (evapotranspiration): Applications

    Institute of Scientific and Technical Information of China (English)

    LIU diaHong; QIN DaYong; WANG MingNa; L(U) JinYan; SANG XueFeng; ZHANG RuiMei

    2009-01-01

    The regional objective ET (evapotranspiration) is defined as the quantity of water that could be con-sumed in a particular region. It varies with the water conditions and economic development stages in the region. It is also constrained by the requirement of benign environment cycle. At the same time, it must meet the demands of sustainable economic growth and the construction of harmony society.Objective ET based water resources distribution will replace the conventional method, which empha-sizes the balance between the water demand and the water supply. It puts focus on the reasonable water consumption instead of the forecasted water demand, which is usually greater than the actual one. In this paper, we calculated the objective ET of 2010 year level in Tianjin by an analysis-integra-tion-assessment method. Objective ET can be classified into two parts: controllable ET and uncontrol-lable ET. Controllable ET includes the ET from irrigation land and the ET from resident land, among which the former can be calculated with soil moisture model and evapotranspiration model, while the latter can be calculated by water use ration and water consumption rate. The uncontrollable ET can be calculated with the distributed hydrological model and the remote sensing monitoring model. The two models can be mutually calibrated. In this paper, eight schemes are put forward based on different portfolios of water resources. The objective ET of each scheme was calculated and the results were assessed and analyzed. Finally, an optimal scheme was recommended.

  5. Entre exotisme et nostalgie

    Directory of Open Access Journals (Sweden)

    Caroline Rozenholc

    2008-03-01

    Full Text Available En 1992, la municipalité de Tel-Aviv Jaffa, sollicitée par un groupe d’habitants, décide d’investir dans le quartier de Florentin. Florentin est alors un des quartiers les plus pauvres de la ville et témoigne, comme l’ensemble de la zone administrative sud, d’un état de forte dégradation. En quelques décennies, le quartier aura en effet été littéralement déserté par sa population, laissant vacants des centaines de logements progressivement investis par de petites industries. Le potentiel du quartier est pourtant évident – architecture unique et forte densité commerciale – et les réhabilitations réussies des quartiers de Neve Tseddek et de Sheinkin encouragent les autorités à « remettre Florentin sur la carte ». Cinq ans plus tard, Eytan Fox – plus connu aujourd’hui pour son film « The Bubble » – prend le quartier comme toile de fond pour réaliser une chronique de la jeunesse israélienne : « Florentin ». Pendant trois ans, cette série télévisée va obtenir une audience nationale confirmée par une rediffusion récente. Florentin est alors consacré comme un espace « à part » et devient emblématique d’une urbanité alternative. Paré d’une aura d’exotisme, renforcée par la présence de nombreux migrants africains, Florentin devient ainsi le lieu d’une certaine culture israélienne : celle d’une génération ouverte à l’altérité et qui, tout en étant ancrée dans la réalité du pays, se projette au-delà de ses frontières. Mêlant sens et temporalités, une conjoncture particulière fait donc émerger Florentin comme un label ; vivre à Florentin devient une véritable « déclaration d’intention ».En transition vers une gentrification évidente, comment ce quartier, longtemps marginalisée et qui n’est inclus dans les limites administratives de Tel-Aviv que depuis les années 50, en est venu à former une entité à part entière au sein de l

  6. SEÑALES PRODUCIDAS POR Rhizobium leguminosarum EN LA INTERACCIÓN CON FRIJOL COMÚN (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    María C. Nápoles García

    2016-01-01

    Full Text Available La interacción Rhizobium-leguminosas depende de un complejo intercambio de señales, que se mantiene durante todo el proceso simbiótico y de las que solamente una combinación correcta permitirá una simbiosis eficiente. Estas plantas secretan flavonoides, reconocidos por bacterias compatibles y que inducen sus genes nod. Estos codifican las proteínas que sintetizan y exportan lipoquitooligosacáridos conocidos como factores Nod. Los factores Nod activan los procesos de infección e inician la división celular en la raíz, hasta la formación del nódulo y participan también en la fijación biológica del nitrógeno. Existen evidencias de que el uso de inductores de los genes nod incrementa la nodulación en algunas leguminosas. El objetivo de este trabajo fue estudiar la producción de algunas moléculas señales, inducidas por la isoflavona genisteína en una cepa de R. leguminosarum y evaluar el impacto de esa inducción en el efecto del inóculo sobre plantas de frijol común. La fracción lipídica en los inóculos fue extraída con n-butanol y analizada por cromatografía de capa fina, cromatografía líquida de alta resolución y cromatografía gaseosa acoplada a un espectrómetro de masas. En los inóculos inducidos con genisteína se detectó una cantidad superior de lipooligosacáridos (factores de nodulación y de ácidos grasos de alto peso molecular, con diferencias significativas respecto a los controles sin inducir. En relación con ese enriquecimiento en moléculas señales, los inóculos inducidos con genisteína, mostraron un efecto positivo en las plantas de frijol de la variedad Cubacueto 25-9, con mayor número de nódulos y contenido de clorofila que las plantas no inoculadas (control.

  7. Terrorisme global et territoire

    OpenAIRE

    Kastoryano, Riva

    2011-01-01

    Qu’il s’agisse du « régime de Terreur » de Robespierre, des attaques du 11 septembre 2001, des actions révolutionnaires nationalistes à la fin de l’empire ottoman ou de la lutte des « combattants de la liberté » contre les pouvoirs coloniaux, toute action caractérisée par la violence est qualifiée de « terroriste ». Le concept, vague et évolutif, est appliqué aux États, aux groupes et aux individus. Les États ont d’ailleurs chacun leur définition du mot, privilégiant leurs intérêts particulie...

  8. Suspicion et exception

    OpenAIRE

    Bigo, Didier; Bonditti, Philippe; Bonelli, Laurent; GARCIA CASTRO, Antonia; Guild, Elspeth; Guittet, Emmanuel-Pierre; Perier, Miriam; Walker, R.B.J.

    2005-01-01

    Sur fond de "guerre globale au terrorisme", les doctrines de sécurité nationale se nourrissent des logiques de l'exception où la sécurité devient la valeur clé, reléguant ainsi la liberté et la justice à d'autres temps. Privilégiant une compréhension des politiques antiterroristes et de leurs effets sur la cohésion sociale, ce numéro apporte un éclairage renouvelé de ces "réponses" politiques qui conduisent, sous couvert d'une conception extensive de l'urgence, à faire de la suspicion une "ce...

  9. Cyberespace et cyberculture

    Directory of Open Access Journals (Sweden)

    Pierre Lévy

    1999-01-01

    Full Text Available Alors, pendant la petite heure que va durer cette conférence, je voudrais partager avec vous quelques réflexions et quelques hypothèses sur la Cyberculture. Je voudrais d'abord situer les choses, mon propos est un propos de philosophe, autrement dit, il va s'agir de spéculations sur des concepts. Donc, je vous avertis tout de suite à quelle sauce vous allez être mangés. Il ne s'agit pas d'un discours culturel critique, comme les intellectuels français en ont l'habitude, et il ne s'agit pas non plus de grandes prédictions sur le XXIème siècle, il s'agit vraiment d'une réflexion un peu approfondie sur des concepts.

  10. Elites judiciaires et culture

    OpenAIRE

    Umbrecht, Véronique

    2012-01-01

    Les magistrats du Conseil souverain d’Alsace ont marqué leur appartenance à l’élite parlementaire française en se constituant de grandes bibliothèques privées. Oscillant entre une bibliothèque professionnelle et de loisirs, ces collections attestent de la forte imprégnation du français et affichent une évolution dans le choix des thématiques. Au cours du XVIIIe siècle, s’amorce un changement dans les pratiques de lecture. L’émergence de sociétés de lecture à Colmar dénote les besoins d’échang...

  11. Cyrano et les hydropotes

    Directory of Open Access Journals (Sweden)

    Alain Mothu

    2007-10-01

    Full Text Available « Car il sera grand devant le Seigneur. Il ne boira ni vin, ni liqueur enivrante, et il sera rempli de l’Esprit-Saint dès le sein de sa mère »(Luc, I, 15Cyrano dédie sa lettre à la gloire de l’aqueduc d’Arcueil et des fontaines parisiennes qu’il alimentait, à « (ses amis les buveurs d’eau ». Cette dédicace abrite peut-être une fine allusion au fait que la construction de l’« acqueduc Médicis », entamée en 1612, fut financée en partie par un droit d’entrée à Paris imposé sur les vins, comme ...

  12. Musique et informatique

    OpenAIRE

    Charnasse, Hélène

    1988-01-01

    Sommaire du numéro :http://archive-edutice.ccsd.cnrs.fr/edutice-00000838 Au sein des Sciences Humaines, la musique constitue un cas très particulier. D'une part, le langage musical peut se suffire à lui-même (c'est le cas des œuvres instrumentales). Ayant son vocabulaire et ses règles syntaxiques, il relève d'études de type sémiologique et linguistique. D'autre part, ce langage peut s'appuyer sur un support littéraire (cas des œuvres vocales). Ce support est alors justiciable d'une étude d...

  13. Musique, chansons et bruits

    OpenAIRE

    Read, Peter

    2016-01-01

    Selon Louise Faure-Favier, « Apollinaire ne cachait pas son ignorance de l’art musical et qu’il ne l’intéressait que dans ses rapports avec la poésie. » Elle reconnaît, cependant, l’admiration qu’il portait à Erik Satie et le montre très ému par la musique de Schumann, jouée au piano par son ami Edgar Varèse. Selon Georges Auric, Apollinaire se souciait peu de la « grande musique », appréciait quelques lie- der de Schubert, mais avait « conservé longtemps le souvenir, pour lui ahurissant, d’u...

  14. Imprudence et impudence

    OpenAIRE

    Thirouin, Laurent

    2016-01-01

    L’art polémique de Pascal dans Les Provinciales se caractérise, selon Jean Mesnard, par deux moyens rhétoriques : « l’expression directe d’une conviction forte », et l’ironie, « à la manière complète qui est celle de Platon ». Qu’est‑ce que cette ironie complète ? (je parlerais volontiers ici d’une ironie authentique, pour la distinguer de toutes les formes dégradées et dérivées, les plus communes, que l’on gratifie habituellement du nom d’ironie). J. Mesnard précise sa définition, en ébaucha...

  15. Et bedre image

    DEFF Research Database (Denmark)

    Vestergaard, Hedvig; Haagerup, Christian Deichmann

    2010-01-01

    Pjecen sammenfatter erfaringer og råd om, hvordan man kan tage fat på at forbedre et boligområdes image. Det er erfaringer og råd som aktører og ledere fra Bispehaven i Århus Kommune, Vejleåparken i Ishøj Kommune og Tingbjerg i Københavns Kommune gerne vil give videre til andre, der skal i gang med...... imageforbedrende indsatser. De tre områder har arbejdet på at forbedre omdømmet og få et bedre image indadtil og udadtil i forbindelse med større renoveringsindsatser i perioden 2002-2009. Hertil kommer optimering af organisationen og organisationskulturen for at nå bedre resultater. Indsatserne i områderne viser...

  16. Candide et le physicien

    CERN Document Server

    Espagnat, Bernard d'

    2008-01-01

    Les avancées considérables de la physique d'après guerre n'ont - on le sait - été possibles qu'au prix d'une vraie rupture entre elle et la physique dite " classique ". A quels changements cette rupture nous contraint-elle en ce qui concerne des notions essentielles, telles celles d'espace, de temps, d'objet et d'objectivité ? Quelles en sont les incidences quant à la portée de la connaissance, au rôle de la conscience, aux relations entre science et ontologie ? Ce sont là des questions de fond, fort délicates, auxquelles les personnes de tendance quelque peu philosophique sont plus sensibles que ne le sont les physiciens. Malheureusement, les premières ne disposent normalement pas des connaissances de pointe qui seules permettent d'approfondir de tels problèmes sans trop risquer de s'égarer. Etant donné l'impact que, par ses applications, la science a sur nos vies, nombreux sont ceux qui souhaitent se former une idée plus juste de ce qu'elle implique vraiment. Le présent ouvrage prend la for...

  17. Un nouvel habitat du Bronze final IIIb dans le Val d’Orléans et ses traces de métallurgie du fer : Bonnée, Les Terres à l’Est du Bourg (Centre, Loiret A new settlement from late Bronze IIIb in the val d’Orléans: Bonnée, Les Terres à l’Est du Bourg (lands to the east of the town (Centre, Loiret

    Directory of Open Access Journals (Sweden)

    Stéphane Joly

    2011-12-01

    Full Text Available La multiplication récente des diagnostics archéologiques autour du petit bourg actuel de Bonnée a permis la détection d’une occupation du Bronze final IIIb (circa 900-750 av. n.-e. sur environ un hectare aux Terres à l’Est du Bourg. Les limites inhérentes à ce type d’intervention réduisent l’interprétation des structures et toute approche spatiale de ce probable habitat repéré. L’étude conjointe des différents mobiliers en particulier céramique et métallurgique, mais aussi le torchis et la faune, atteste de sa culture matérielle et de son intérêt. Ces scories pourraient être parmi les plus anciennes traces en région Centre d’activité métallurgique du fer. Des activités de post-réduction sont avérées et certaines opérations de forgeage sont supposées.La découverte de cet habitat dans ce secteur du lit majeur de la Loire, apporte de nouvelles données concernant l’occupation du sol et ses problématiques évolutives dans le Val d’Orléans sur ces périodes de transition avec le Hallstatt ancien.The recent increase in the archaeological evaluations around the village of Bonnée has enabled the discovery of a final Bronze Age IIIb settlement (circa 900-750 BP on about a hectare of the Terres à l’Est du Bourg. The limits inherent in this type of work restrict the interpretation of structures and all spatial approaches to this likely dwelling. The joint study of the different furnishings, in particular ceramics and metal, but also the cob and fauna, attests to its material culture and its interest. The slag could be amongst the oldest iron metallurgy remains from the Centre region. Some activities of post-forging are proven and certain operations are guessed at. The discovery of this settlement in this sector of the major bed of the Loire, has produced new data concerning the occupation of the land and its evolutionary problems in the valley of Orleans over these periods of transition in the early

  18. Étude dans les états finals dileptoniques de différentes propriétés des paires top-antitop avec les détecteurs D0 et ATLAS; Study in the final states dileptoniques different properties of top-antitop pairs with D0 and ATLAS detectors

    Energy Technology Data Exchange (ETDEWEB)

    Deterre, Cecile [Univ. Pierre et Marie Curie, Paris (France)

    2012-06-26

    Studies of different properties of the top-antitop pairs in dileptonique final state are presented in this thesis. Two analyzes were conducted in different experiments: one to D0 at the Tevatron, the other in ATLAS at LHC. Both colliders are different, the measures undertaken are complementary to the top of the properties of the study. The first analysis, conducted in the D0 experiment was to make simultaneous measurements of the cross section for production of pairs of top-antitop and branching ratio t -> Wb This was carried out in the channel with a dileptonique batch of data corresponding to a brightness of 5.4 fb⁻¹. It was then combined with the measurement made in the semileptonique channel to obtain an accuracy of 8% over the cross section, comparable to the accuracy of theoretical calculations. The second analysis presented, conducted in the ATLAS experiment was to measure the top quark charge asymmetry in dileptonique channel with the data set recorded by ATLAS in 2011, or 4.7 fb⁻¹. The results were then combined with the results of semileptonique channel obtained with 1 fb⁻¹. We measure: AC (ttbar) = 0.029 ± 0.018 (stat.) ± 0.014 (syst.), Which is compatible with the standard model prediction of 0.004 ±- 0.001.

  19. Search for h and A neutral Higgs bosons in final states with 4 jets in the experimental data collected by DELPHI detector at LEP200; Recherche de bosons de higgs neutres h et A dans les etats finals a quatre jets, avec le detecteur DELPHI a LEP200

    Energy Technology Data Exchange (ETDEWEB)

    Boonekamp, M. [CEA/Saclay, Dept. d' Astrophysique, de la Physique des Particules, de la Physique Nucleaire et de l' Instrumentation Associee (DAPNIA), 91 - Gif-sur-Yvette (France)]|[Paris-7 Univ., 75 (France)

    1999-05-01

    Searches for Higgs bosons are presented in the framework of the standard model, of the minimal supersymmetric model, and of itsfirst extension. The data sample used has been collected by Delphi in 1997 and 1998, and represents an integrated luminosity of 212 pb{sup -1}. The final states considered are the bb-bar bb-bar decay of the e{sup +}e{sup -} {yields} hZ process. The methods used for the analyses and for the interpretation of their results are described, as well as the implementation of b-quark identification from leptonic decays. After a first selection, the discrimination between background and signal events is studied, and the most powerful quantities are combined into a single variable. The best signal sensitivity is obtained by a weighted counting of all events, where the weight of the events is defined on the basis of the single variable. An excess of data, encountered in the search for hZ, justifies a separate discussion. The main instrumental and theoretical uncertainties, from which this excess could originate, are discussed. It is shown that the majority of the events in excess do not correspond to a Higgs signal, and that it is difficult to understand the excess as a consequence of imperfect standard process simulation. The results of the searches allow to give limits on the masses of the Higgs bosons in the models under consideration. The data imply m{sub h}>93 GeV in the standard model. In the MSSM, one finds m{sub h}>82.4 GeV and m{sub h} >83.3 GeV, for any tan{beta}. On the simplest extension of the MSSM, the pseudoscalar mass parameter m{sub h} must be greater than 69 GeV for tan{beta} >1. No constraint is found for tan{beta} <1. All limits are given at 95% C.L. (author)

  20. Immobilization and Survival of Root Nodule Bacterium Rhizobium leguminosarum Biovar viciae / Gumiņbaktērijas Rhizobium leguminosarum biovar viciae imobilizācija un dzīvotspēja

    Directory of Open Access Journals (Sweden)

    Žvagiņa Signe

    2015-11-01

    Full Text Available Rhizobium leguminosarum biovar viciae ir augsnes baktērija, kas spēj veidot gumiņus uz zirņu, pupu, lēcu un vīķu saknēm un saistīt atmosfēras slāpekli simbiozē ar augiem. Gumiņbaktērijas izmanto lauksaimniecībā kā bioloģiskus mēslošanas līdzekļus augu augšanas veicināšanai. Komerciālos gumiņbaktēriju preparātus var iegādāties sausā, šķidrā vai liofilizētā formā. Parasti izmanto sausos sagatavotos mēslošanas līdzekļus uz kūdras bāzes. Šī pētījuma mērķis bija imobilizēt R. leguminosarum un noteikt tās dzīvotspēju šķidrumā un dažādos nesējmateriālos, lai izstrādātu uzlabotus gumiņbaktēriju preparātus. Imobilizēšanai izmantoja piecus sterilizētus materiālus: kūdru, māla pulveri, divu veidu ovālus šūnainās keramikas agregātus un cilindriskas keramikas granulas, kas izgatavotas no Planču depozīta Devona perioda māla. Imobilizāciju veica 2,5 stundu laikā 20 °C temperatūrā. Vēlāk kūdru, pulveri un granulas noberza un sasmalcināja ūdenī ar sterilu piestiņu, lai atdalītu pielipušās baktērijas. Baktēriju kolonijas veidojošo vienību skaitu noteica, izsējot iegūto suspensiju atšķaidījumus Petri traukos ar agarizētu barotni. Dzīvotspēju noteica arī ar LIVE/DEAD epifluorescences metodi. Iegūtie rezultāti parādīja, ka nesējmateriāls ietekmē imobilizācijas sekmes un ka uzglabāšanas temperatūra ietekmē R. leguminosarum dzīvotspēju. Vislabākos rezultātus ieguva, uzglabājot baktērijas suspensijā vai imobilizējot uz kūdras. Rekomendējam uzglabāt R. leguminosarum produktus −18 °C vai 4 °C temperatūrā.

  1. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gurney, Kevin R

    2015-01-12

    This document constitutes the final report under DOE grant DE-FG-08ER64649. The organization of this document is as follows: first, I will review the original scope of the proposed research. Second, I will present the current draft of a paper nearing submission to Nature Climate Change on the initial results of this funded effort. Finally, I will present the last phase of the research under this grant which has supported a Ph.D. student. To that end, I will present the graduate student’s proposed research, a portion of which is completed and reflected in the paper nearing submission. This final work phase will be completed in the next 12 months. This final workphase will likely result in 1-2 additional publications and we consider the results (as exemplified by the current paper) high quality. The continuing results will acknowledge the funding provided by DOE grant DE-FG-08ER64649.

  2. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    DeTar, Carleton [P.I.

    2012-12-10

    This document constitutes the Final Report for award DE-FC02-06ER41446 as required by the Office of Science. It summarizes accomplishments and provides copies of scientific publications with significant contribution from this award.

  3. [Secretion of Phenolic Compounds into Root Exudates of Pea Seedlings upon Inoculation with Rhizobium leguminosarum bv. viceae or Pseudomonas siringae pv. Pisi].

    Science.gov (United States)

    Makarova, L E; Dudareva, L V; Petrova, I G; Vasil'eva, G G

    2016-01-01

    The content of apigenin, naringenin, pisatin, dibutyl-ortho-phthalate, and N-phenyl-2-naphthyl-amine were assayed in root exudates of pea (Pisum sativum L.) seedlings one day after their inoculation with Rhizobium leguminosarum, bv. viceae or Pseudomonas siringae pv. pisi, which represent, respectively, mutualistic and antagonistic strategies of interaction with a host plant. After inoculation with either bacteria, the concentrations of apigenin and pisatin in the root exudates were equal, whereas the concentrations of naringenin and N-phenyl-2-naphthylamine were different and those of dibutyl-o-phthalate were unchanged. A certain role is suggested for the phenolic compounds in an accomplishment of symbiotic relations of bacteria with a host plant. PMID:27266251

  4. Co-inoculation with Rhizobium and plant growth promoting rhizobacteria (PGPR for inducing salinity tolerance in mung bean under field condition of semi arid climate

    Directory of Open Access Journals (Sweden)

    Muhammad Aamir

    2013-04-01

    Full Text Available Salinity stress severely affects the growth, nodulation and yield of mung bean (Vigna radiata L.. However, its growth can be improved under salinity stress by inoculation/co-inoculation with rhizobia and plant growth promoting rhizobacteria (PGPR containing 1-Aminocyclopropane-1-carboxylic acid (ACC deaminase enzyme. ACC-deaminase containing bacteria regulate the stress induced ethylene production by hydrolyzing the ACC (immediate precursor of ethylene into ammonia and ketobutyric acid, thus improve plant growth by lowering the ethylene level. A study was conducted under salt affected field conditions where pre-isolated strains of Rhizobium and PGPR were used alone as well as in combination for mitigating the salinity stress on growth, nodulation and yield of mung bean by following the randomized complete block design (RCBD. The data were recorded and analyzed statistically to see the difference among treatments.

  5. [Secretion of Phenolic Compounds into Root Exudates of Pea Seedlings upon Inoculation with Rhizobium leguminosarum bv. viceae or Pseudomonas siringae pv. Pisi].

    Science.gov (United States)

    Makarova, L E; Dudareva, L V; Petrova, I G; Vasil'eva, G G

    2016-01-01

    The content of apigenin, naringenin, pisatin, dibutyl-ortho-phthalate, and N-phenyl-2-naphthyl-amine were assayed in root exudates of pea (Pisum sativum L.) seedlings one day after their inoculation with Rhizobium leguminosarum, bv. viceae or Pseudomonas siringae pv. pisi, which represent, respectively, mutualistic and antagonistic strategies of interaction with a host plant. After inoculation with either bacteria, the concentrations of apigenin and pisatin in the root exudates were equal, whereas the concentrations of naringenin and N-phenyl-2-naphthylamine were different and those of dibutyl-o-phthalate were unchanged. A certain role is suggested for the phenolic compounds in an accomplishment of symbiotic relations of bacteria with a host plant.

  6. Et Murstenstårn

    DEFF Research Database (Denmark)

    Jensen, Thomas Bo

    2000-01-01

    Beskrivelse af et kunstnerisk udviklingsarbejde i forbindelse med phd-afhandlingen ”Murstenens ornamentale vilje".......Beskrivelse af et kunstnerisk udviklingsarbejde i forbindelse med phd-afhandlingen ”Murstenens ornamentale vilje"....

  7. Des racines et des ailes

    Directory of Open Access Journals (Sweden)

    Stéphanie Vincent-Geslin

    2012-05-01

    Full Text Available Les mobilités pendulaires semblent être en augmentation en Europe depuis une dizaine d’années. Cette croissance du temps passé à se déplacer amène à remettre en question la conjecture de Zahavi et apparaît relativement inexplicable en regard du paradigme classique de l’acteur rationnel traditionnellement utilisé dans le champ des transports. Si, dans la littérature, les temps de déplacements sont principalement expliqués par le contexte résidentiel, la forme urbaine et le travail, ce cadre explicatif ne dit rien des processus de décision eux-mêmes qui amènent aux pendularités intensives.À partir d’une enquête qualitative menée auprès de pendulaires français, suisses et belges, cette contribution propose d’analyser les arbitrages et les éléments déterminants des processus de la grande pendularité. Les mobilités quotidiennes pendulaires apparaissent comme le résultat de compromis entre activité professionnelle, attachement résidentiel et choix de vie et prennent ainsi la forme de stratégies de conciliation entre vie privée et vie professionnelle. Ces mobilités spatiales permettent alors paradoxalement la préservation des ancrages résidentiels, sociaux et familiaux.Roots and wings. Long-distance commuting patterns, or how to conciliate professional and personal lifeLong-distance commuting patterns appear to be increasing in Europe over the last ten years. These raising mobility patterns lead to reappraise the Zahavi conjecture and appear largely inexplicable by the classical rational actor paradigm traditionally used in transportation research. In literature, commuting is mainly explained by residential contexts, urban forms and job. Nevertheless this theoretical frame says little about the decision-making processes themselves. Based on a qualitative survey conducted in three European countries - France, Belgium and Switzerland – among a population of high commuters, this paper proposes an analysis of

  8. Capital social et anthroponomie

    OpenAIRE

    Peaucelle, Irina

    2002-01-01

    Dans ce texte j'analyse les relations entre la composante Santé du capital social et la reproduction élargie de l'économie. Il s'agit aussi de discuter des méthodes d'évaluation de santé des populations pour apprécier leur potentiel de travail. En s'appuyant sur des travaux en psychologie sociale, je cherche à appréhender les rythmes anthroponomiques de l'évolution économique.

  9. Sources et bibliographie

    OpenAIRE

    2015-01-01

    SOURCES Sources Archivistiques Archives nationales (AN) Série AP. Archives personnelles et familiales AP/09. Fonds privés d’avocats. 304/ AP. Maurice Garçon. 334/AP. Bluet. 417/AP. Georges Coulon. 470/AP. Alexandre Millerand. 476/AP. Chauveau- Lagarde. 488/AP. Joseph Denaïs. 505/AP. Edgar Faure. 516/AP. Bourdeau de Fontenay. 556/AP. Gabriel Olivier. 596/AP. Georges Calzant. Série BB. Ministère de la Justice BB29 : Enregistrement général de la correspondance BB29/723-724. Matricule des avoués ...

  10. Musique et holographie

    OpenAIRE

    Vach, Holger

    2010-01-01

    Introduction L’holographie est surtout connue pour son rendu esthétique dans l’enregistrement d’images en trois dimensions. Cependant, dans le contexte des mesures scientifiques, elle joue souvent le rôle d’un instrument à la fois précis et simple d’emploi, par exemple pour les mesures de tout petits déplacements, ou de changements d’index de réfraction, ou bien d’amplitudes de vibration. Dans ce dernier cas, l’hologramme d’un objet en vibration présente l’objet en trois dimensions superposé ...

  11. Musique et pouvoirs

    OpenAIRE

    Aubert, Laurent; Bois, Pierre; Borel, François; Brandily, Monique; CAUFRIEZ, Anne; Cheyronnaud, Jacques; Crowe, Peter; Desjacques, Alain; During, Jean; Erguner, Ahmed Kudsi; Helffer, Mireille; Howard, Keith; Leymarie, Isabelle; Lortat-Jacob, Bernard; Mesnil, Marianne

    2015-01-01

    Selon une croyance quasi universelle, certaines musiques sont investies de pouvoirs, d'où leur efficacité dans des rites permettant à l'individu ou à la collectivité de communiquer avec le monde invisible. D'autres musiques sont porteuses de messages subliminaux et d'effets, dans la mesure où leurs formes sont adaptées à leur finalité. Ainsi, l'usage de la musique à des fins politiques est fréquent, en tant que signe du pouvoir ou que de véhicule de sa propagation. Cette relation peut d'aille...

  12. Corpus et stylistique

    OpenAIRE

    Magri-Mourgues, Véronique

    2007-01-01

    Une question lancée comme un défi a sous-tendu ce numéro de Corpus. Parallèlement aux linguistiques de corpus qui commencent à être clairement établies, serait-il envisageable de définir une stylistique de corpus ? Ou bien formulé autrement, que peut être un corpus pertinent en stylistique ? Quelles sont ses spécificités, si elles existent ? Articulant la problématique du corpus et l’analyse stylistique, les contributeurs explorent ce faisant des enjeux épistémologiques qui font envisager un ...

  13. Anatomie et identification des bois

    OpenAIRE

    Jourez, Benoît

    2010-01-01

    Anatomie des bois Structure anatomique des résineux et des feuillus Structure de la membrane cellulaire structure submicroscopique Anatomie du bois des essences feuillues tropicales Caractères anatomiques servant à l'identification des essences Reconnaissance microscopique du bois des essences résineuses et feuillues Duramen et duraminisation Formations anormales ( bois de compression et bois de tension) Chimie du bois Composition générale Cellulose, hé...

  14. FINAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    Lettenmaier, Dennis [University of Washsington

    2012-05-26

    We proposed to extend Maurer’s data sets through at least 2005 (to include extreme drought years in the Colorado basin). We updated and verified the forcings for tmin, tmax, and precipitation over the Colorado River basin at 1/8-deg spatial resolution through November 2008, with the potential to alter the resolution as needed (we subsequently extended the Maurer et al data set over the entire continental U.S. at 1/16 degree spatial resolution; see Livneh et al., 2013). We proposed to use either MODIS-based land cover data for recent years, or modification of the existing fixed seasonal cycle used in VIC (based on University of Maryland land cover data) to represent interannual variations in vegetation characteristics such as leaf area index (LAI) particularly in drought years. We assessed model performance with respect to evapotranspiration estimation through comparison of the model predictions with ground observations and in experiments that use time-varying and fixed seasonal LAI cycles (based on University of Maryland land cover data) in a test region of northwestern Mexico where the ground ET observations from eddy covariance tower sites are available for the period from 2001 to 2008 (Tang et al., 2011). We also proposed to implement statistical downscaling with an adjustment to constrain precipitation changes at the GCM level. These simulations were performed, using 20 IPCC AR4 GCMs over the Colorado River basin with two global emissions scenarios, and are reported in Vano et al., 2014. Task 2: Coupled model implementation We proposed to implement the “standard” climate version of WRF, as used by collaborator Ruby Leung in NARCCAP simulations (see Section 5.4), and perform tests to assure that model output for runs equivalent to NARCCAP Phase 1 (reanalysis boundary conditions) are consistent. We proposed do test sensitivity to higher spatial resolution. We made a run of 11 years’ length with the “standard” version of WRF, forced by NCEP/DOE with

  15. 根瘤菌、微肥和作物生长调节剂对大豆氮磷钾积累和产量的影响%Influence of Rhizobium, Trace Fertilizer and Crop Growth Regulators on Nitrogen,Phosphorous, Potassium Accumulation and Yield of Soybean

    Institute of Scientific and Technical Information of China (English)

    金晓梅; Синеговская В Т; 赵念力

    2009-01-01

    采用根瘤菌、植物生长调节剂、单一及复合肥料应用试验,研究其增产效果,寻找最佳增产方案.在全俄大豆研究所的2年试验结果表明:在大豆整个生长过程中,不同处理N、P_2O_5和K_2O的积累量与地上部于物重呈极显著线性相关,大豆对N、P_2O_5 和K_2O吸收比例为8.34:1.00:3.42;各处理产量均比对照(CK)明显增加,增幅达12%~25%.表现最佳的处理为混合制剂(钼酸铵、根瘤菌、生长调节剂),其干物质积累比CK提高55.4%,产量增加25%.%Different fertilizer treatments, including seed coated with ammonium molybdate ( T1) , rhizobium ( T2 ) , plant growth regulator( T3 ) , mixture of ammonium molybdate, rhizobium and plant growth regulator( T4 ) as well as starter fertilizer of N 17 kg · ha~(-1) and P_2O_5 60 kg · ha~(-1) (T5) were conducted at the whole Russia Soybean Institute experimental station for two years, and their effects on the dynamic accumulation of nitrogen, phosphorous, potassium and final seed yield were determined. Under different treatment,the accumulation of N,P_20_5and K_2O showed positive linear correlation with above-ground biomass,and the ratio of absorbed elements was 8.34 N:1.00 P_2O_5:3.42K_2O. All treatments increased seed yield from 12% to 25% compared with CK. T4 treatment had the best effect,under which dry matter increased 55.4% ,yield increased 25 %.

  16. Multiple ETS family proteins regulate PF4 gene expression by binding to the same ETS binding site.

    Directory of Open Access Journals (Sweden)

    Yoshiaki Okada

    Full Text Available In previous studies on the mechanism underlying megakaryocyte-specific gene expression, several ETS motifs were found in each megakaryocyte-specific gene promoter. Although these studies suggested that several ETS family proteins regulate megakaryocyte-specific gene expression, only a few ETS family proteins have been identified. Platelet factor 4 (PF4 is a megakaryocyte-specific gene and its promoter includes multiple ETS motifs. We had previously shown that ETS-1 binds to an ETS motif in the PF4 promoter. However, the functions of the other ETS motifs are still unclear. The goal of this study was to investigate a novel functional ETS motif in the PF4 promoter and identify proteins binding to the motif. In electrophoretic mobility shift assays and a chromatin immunoprecipitation assay, FLI-1, ELF-1, and GABP bound to the -51 ETS site. Expression of FLI-1, ELF-1, and GABP activated the PF4 promoter in HepG2 cells. Mutation of a -51 ETS site attenuated FLI-1-, ELF-1-, and GABP-mediated transactivation of the promoter. siRNA analysis demonstrated that FLI-1, ELF-1, and GABP regulate PF4 gene expression in HEL cells. Among these three proteins, only FLI-1 synergistically activated the promoter with GATA-1. In addition, only FLI-1 expression was increased during megakaryocytic differentiation. Finally, the importance of the -51 ETS site for the activation of the PF4 promoter during physiological megakaryocytic differentiation was confirmed by a novel reporter gene assay using in vitro ES cell differentiation system. Together, these data suggest that FLI-1, ELF-1, and GABP regulate PF4 gene expression through the -51 ETS site in megakaryocytes and implicate the differentiation stage-specific regulation of PF4 gene expression by multiple ETS factors.

  17. Characterization and functional analysis of seven flagellin genes in Rhizobium leguminosarum bv. viciae. Characterization of R. leguminosarum flagellins

    Directory of Open Access Journals (Sweden)

    Tambalo Dinah D

    2010-08-01

    Full Text Available Abstract Background Rhizobium leguminosarum bv. viciae establishes symbiotic nitrogen fixing partnerships with plant species belonging to the Tribe Vicieae, which includes the genera Vicia, Lathyrus, Pisum and Lens. Motility and chemotaxis are important in the ecology of R. leguminosarum to provide a competitive advantage during the early steps of nodulation, but the mechanisms of motility and flagellar assembly remain poorly studied. This paper addresses the role of the seven flagellin genes in producing a functional flagellum. Results R. leguminosarum strains 3841 and VF39SM have seven flagellin genes (flaA, flaB, flaC, flaD, flaE, flaH, and flaG, which are transcribed separately. The predicted flagellins of 3841 are highly similar or identical to the corresponding flagellins in VF39SM. flaA, flaB, flaC, and flaD are in tandem array and are located in the main flagellar gene cluster. flaH and flaG are located outside of the flagellar/motility region while flaE is plasmid-borne. Five flagellin subunits (FlaA, FlaB, FlaC, FlaE, and FlaG are highly similar to each other, whereas FlaD and FlaH are more distantly related. All flagellins exhibit conserved amino acid residues at the N- and C-terminal ends and are variable in the central regions. Strain 3841 has 1-3 plain subpolar flagella while strain VF39SM exhibits 4-7 plain peritrichous flagella. Three flagellins (FlaA/B/C and five flagellins (FlaA/B/C/E/G were detected by mass spectrometry in the flagellar filaments of strains 3841 and VF39SM, respectively. Mutation of flaA resulted in non-motile VF39SM and extremely reduced motility in 3841. Individual mutations of flaB and flaC resulted in shorter flagellar filaments and consequently reduced swimming and swarming motility for both strains. Mutant VF39SM strains carrying individual mutations in flaD, flaE, flaH, and flaG were not significantly affected in motility and filament morphology. The flagellar filament and the motility of 3841 strains

  18. Acta Aeronautica et Astronautica Sinica

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@@@ABSTRACTS Motion Estimation and Compensation Based on Trifocal Tensor and BMA.WANG Yang-li, WU Cheng-ke(School of Information Engineering, Xidian University, Xi′an 710071, China).Acta Aeronautica et Astronautica Sinica(ISSN 1000-6893),Vol.22,no.2,Mar.2001,P117-120. 10 refs.in Chinese. To overcome some drawbacks of the block matching algorithm (BMA), which can not accurately describe object rotation, camera panning and zooming, this paper introduces the trilinear constraint and trifocal-tensor-based pixel transfer used in computer vision for motion estimation and compensation. In this method, the trifocal tensor is first estimated by an algorithm based on projection matrix, corresponding relations between two of three images are then determined by BMA and represented with block-based motion vectors, and finally, motion estimation and compensation are carried out by trifocal transferring. As it is not accurate to approximate the dense correspondence between two of the three views by the above block-based motion vectors, using trifocal transferring only will not yield good compensation results. To solve this problem, the paper further puts forward a motion estimation method combining trifocal transferring with BMA. Experiments have been carried out on video sequences to compare the performance of this method with that of BMA, and the results show that this method is superior when there are scene depth variation and camera translation.

  19. Optimisation combinatoire Theorie et algorithmes

    CERN Document Server

    Korte, Bernhard; Fonlupt, Jean

    2010-01-01

    Ce livre est la traduction fran aise de la quatri me et derni re dition de Combinatorial Optimization: Theory and Algorithms crit par deux minents sp cialistes du domaine: Bernhard Korte et Jens Vygen de l'universit de Bonn en Allemagne. Il met l accent sur les aspects th oriques de l'optimisation combinatoire ainsi que sur les algorithmes efficaces et exacts de r solution de probl mes. Il se distingue en cela des approches heuristiques plus simples et souvent d crites par ailleurs. L ouvrage contient de nombreuses d monstrations, concises et l gantes, de r sultats difficiles. Destin aux tudia

  20. Musique et genre en sociologie

    OpenAIRE

    Prévost-Thomas, Cécile; Ravet, Hyacinthe

    2009-01-01

    Cet article recense et présente brièvement les recherches et publications sociologiques francophones les plus récentes consacrées aux rapports entre genre et musique selon trois grandes thématiques : celle de la création musicale au féminin, celle de la condition des musiciennes, et celle de la voix des femmes qui, plus étoffée, est également abordée sous l’angle des disciplines anthropologique et musicologique. Chacune de ces thématiques est traversée par la question des enjeux symboliques d...

  1. Bouvard et Pécuchet et le désir amoureux

    Directory of Open Access Journals (Sweden)

    Anne Herschberg Pierrot

    2010-09-01

    Full Text Available L’article propose une étude de genèse du chapitre 7 de Bouvard et Pécuchet, consacré à l’amour, qui forme une étape encyclopédique a priori un peu surprenante. Flaubert s’amuse à écrire ces aventures de Bouvard et Pécuchet, dans un pays qui n’est pas si tendre. L’étude de genèse porte sur le travail de l’écriture de plusieurs temps forts du chapitre (dans l’incipit, et les scènes de duos, notamment la scène entre Mme Castillon et Gorgu. Elle permet de montrer l’effacement de détails érotiques, mais aussi du politique et de l’histoire, qui se trouvent condensés dans le texte final. Elle révèle aussi le rôle concerté de ce chapitre dans la construction du roman et l’itinéraire des personnages. Anodin en apparence, ce chapitre est emblématique d’une poétique fondée sur l’ellipse, et qui mêle le jeu et le sérieux.This article presents a textual genetics study of Bouvard et Pécuchet’s 7th chapter, about love, which appears to be a surprising encyclopedic stage of the novel. Flaubert has fun writing these adventures in a not so tender country. This genetics study focuses on the writing of several key moments of the chapter (in the beginning and the duo scenes, such as the one between Mme Castillon and Gorgu. It reveals the erasure of erotic details, as well as politics and history, which end up condensed in the final text. It also shows this chapter’s concerted role in the construction of the novel and the characters’ development. Apparently minor, this chapter is emblematic of a poetics founded on the ellipsis, mingling play and seriousness.

  2. Crime, violence et ville

    OpenAIRE

    Peralva, Angelina; Telles, Vera da Silva

    2014-01-01

    En mai 2006 São Paulo, capitale de l’État fédéré brésilien de même nom, s’est trouvée quasiment paralysée pendant cinq jours. Les commerces ont baissé le rideau, les piétons ont déserté les rues, les voitures et les transports collectifs ont cessé de circuler. Une ville fantôme au cœur d’une métropole de presque 20 millions d’habitants. A l’origine de ce phénomène, un ensemble d’actions violentes désignées par la presse comme « les attaques du PCC ». Le sigle évoque le « Primeiro Comando da C...

  3. Tourisme, patrimonialisation et politique

    OpenAIRE

    Boullosa-Joly, Maïté

    2013-01-01

    La « Fête nationale de la Pachamama » qui se déroule dans le nord-ouest argentin à Amaicha, est un exemple de « co-constitution touristique et patrimoniale » [Lazzarotti, 2001] aux enjeux éminemment politiques. Nous montrons en effet comment la mise en scène d’une culture dans un projet de développement touristique a participé à ériger une croyance, celle en la Pachamama (la Mère Terre), pourtant commune au monde andin, en un « patrimoine culturel » singulier à ce village. Cette valorisation ...

  4. Droit et travail

    OpenAIRE

    Chambost, Isabelle; Dressen, Marnix; Maugeri, Salvatore; Touchelay, Béatrice

    2015-01-01

    Une des motivations de ce Corpus consacré aux rapports entre le droit et le travail renvoie assurément aux lois Auroux de 1982, dont on a célébré il y a peu le quarantième anniversaire. Ces lois garantissaient aux salariés de meilleurs droits ainsi qu’une plus grande capacité à accéder à des informations touchant à la marche de l’entreprise (notamment grâce au renforcement des prérogatives économiques des comités d’entreprise). On pouvait ainsi espérer l’inscription du droit du travail dans u...

  5. Formatversionering. Et overblik

    DEFF Research Database (Denmark)

    Jensen, Pia Majbritt

    2005-01-01

    Formatversionering spiller en stadig større rolle i den internationale tv-branche. Formater som "Big Brother", "Robinson", "Hvem vil være millionær", "Vild med dans" og "Par på prøve" findes på lokale tv-skærme i store dele af verden. En rapport fastslog for nylig, at formathandlen alene i Danmark...... med alternativerne, egenproduktion og indkøb af originale udenlandske programmer? Herefter argumenteres der for at se formaterne som del af en transnational mediekultur, i og med at de på den ene side er et resultat af den økonomiske, teknologiske og kulturelle globalisering, mens de på den anden side...

  6. Pareto et Ernest Roguin

    OpenAIRE

    Tappy, Denis

    2013-01-01

    Vie et œuvre d’Ernest Roguin Selon une recherche sur Internet, le plus récent ouvrage consacrant des développements substantiels à Ernest Roguin semble être une traduction en portugais, parue il y a quelques mois au Brésil, d’un livre du grand philosophe du droit italien Norberto Bobbio, récemment décédé. Il s’agit essentiellement d’une présentation de l’œuvre de Kelsen, qui s’achève cependant par un chapitre entier consacré au Vaudois, sous le titre révélateur de « Um theorico di direito esq...

  7. LASER ET GLAUCOMES

    OpenAIRE

    Sellem, E.-A.

    1987-01-01

    Très schématiquement, les glaucomes peuvent être définis comme des maladies ophtalmologiques dans lesquelles la pression intra-oculaire (PIO) est trop élevée, ce qui provoquera plus ou moins rapidement une destruction des fibres optiques et un risque de cécité. L'humeur aqueuse est directement responsable de la valeur de la PIO. Elle est sécrétée par le corps ciliaire, passe dans la chambre antérieure au travers de la pupille, pour être évacuée au niveau de l'angle irido-cornéen par le trabec...

  8. Genre, raison et nation

    Directory of Open Access Journals (Sweden)

    Rada Ivekovic

    2009-01-01

    Full Text Available Ce travail s’intéresse aux mécanismes de sexuation et de subjectivation dans la construction de la nation. Quelques-uns ont postulé le “retard” de certains groupes sociaux par rapport à l’avènement de la “modernité”, concernant notamment les sujets extraeuropéens, mais aussi les femmes. En réalité, il n’y a pas de retard soit des femmes, soit des peuples colonisés en matière de modernité, parce que la norme de celle-ci est établie elle-même de façon arbitraire à partir d’une position de domination hégémonique depuis la modernité occidentale.

  9. Braille et la musique

    OpenAIRE

    Ar Rouz, David

    2014-01-01

    La musique, universelle ? Voire ! Il n’est qu’à écouter une Suite pour violoncelle de Bach sur des enceintes fabriquées par un maître chinois, chanteur d’opéra, pour comprendre qu’à l’autre bout de la planète, ils n’entendent décidément pas comme nous : pour faire court, voire simpliste, ils aiment les sons « acides » quand nous avons tendance à chercher d’abord des basses rondes. Et combien de styles de musique, même sans aller chercher les pays les plus lointains, sommes-nous incapables d’a...

  10. Nietzsche et la musique

    OpenAIRE

    Montebello, Pierre

    2005-01-01

    Pourquoi Nietzsche n'abandonna-t-il jamais Wagner même s'il se sépara de lui pour des motifs philosophiques ? On sait que la musique de Wagner a joué le rôle de scène originaire dans la formation d'une pensée qui chercha initialement à comprendre le monde au travers d'une métaphysique esthétique. Il faut ajouter que la musique wagnérienne, en dépit d'une teneur esthétique et dramatique de plus en plus éloignée de ce qu'attendait Nietzsche, fut intégrée dans le centre vivant de la pensée nietz...

  11. At skabe et praksischock!

    DEFF Research Database (Denmark)

    Larsen, Kristian

    2011-01-01

    teori og praksis osv. Det kunne være et ideal at man gjorde en dyd af hvad skole (schole er egentlig fritid fra håndens arbejde) er for en størrelse. Det kunne formuleres tydeligt for de studerende i hver start af en undervisningslektion: ”Nu i dag, skal vi høre om en af flere konkurrerende måder man...... kan tænke om mennesket og dets reaktioner på alvorlig sygdom! Det er en abstraktion, en model/kortlægning som Culberg har udarbejdet. Ud fra hans arbejde fremgik der nogle mønstre i måder mennesker reagerede på. Han forenklede dem og opdelte i fire (chokfasen, reaktionsfasen..). Ved undervisningens...

  12. Détermination expérimentale des isothermes de sorption et de la chaleur isostérique des feuilles d'absinthe et de menthe pouliot

    OpenAIRE

    Lamharrar, Abdelkader; Kane, Cheikh Sidi Ethmane; Idlimam, Ali; Akkad, Siham; Kouhila, Mohammed; Mimet, Abdelaziz; Ahachad, Mohamed

    2007-01-01

    International audience La détermination des isothermes de sorption est une étape indispensable et un moyen privilégié pour connaitre la répartition et l'intensité des liaisons de l'eau dans les produits agro-alimentaires. Ces isothermes permettent de déterminer la teneur en eau finale à atteindre pour optimiser les conditions de stockage et de séchage de ces produits et donnent des informations précieuses sur l'équilibre hygroscopique du produit à sécher et à conserver. Cette étude vise d'...

  13. Final Report

    DEFF Research Database (Denmark)

    Heiselberg, Per; Brohus, Henrik; Nielsen, Peter V.

    This final report for the Hybrid Ventilation Centre at Aalborg University describes the activities and research achievement in the project period from August 2001 to August 2006. The report summarises the work performed and the results achieved with reference to articles and reports published...

  14. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Estep, Donald [Colorado State University

    2015-11-30

    This is the final report for the project titled "Enabling Predictive Simulation and UQ of complex Multiphysics PDE systems by the Development of Goal-Oriented Variational Sensitivity Analysis and a-Posteriori Error Estimation Methods" that ran from 9/1/2010 through 8/31/2015. The report describes the scientific progress, all manuscripts, and records project-related activities.

  15. Effects of Rhizobium and Soil Properties on Yield and Agronomic Characteristics of Mung Bean%根瘤菌剂拌种和土壤条件对绿豆生长发育的影响

    Institute of Scientific and Technical Information of China (English)

    李莉; 万正煌; 仲建锋; 陈宏伟; 伍广洪

    2011-01-01

    探讨了根瘤菌剂拌种和土壤条件对绿豆根系生长、主要农艺性状和产量的影响.结果表明,改良土壤对绿豆的生长有显著的促进作用,能优化绿豆全生育期中侧根总长、根瘤数、株高、地上部分鲜重、有效分枝数和单株荚数等性状的表现;在土壤条件无法改变时,进行根瘤菌剂拌种可使绿豆的结瘤性能增强,并有利于促进绿豆的生长和产量提高.%The effects of Rhizobium and soil properties on root growth, agronomic characteristics and yield of mung bean were studied. The results showed that soil quality improvement could boost mung bean growth significantly or highly significantly, as length of lateral root, nodule number of lateral root, plant height, fresh weight of aboveground biomass, valid branch number and pod per plant was increased. When the soil condition could not be reformed, inoculating Rhizobium could also improve agronomic characteristics and yield of mung bean significantly due to the good nodulating ability of Rhizobium.

  16. 接种耐酸根瘤菌对紫花苜蓿生长的影响%Effect of Acid-tolerant Rhizobium on the Growth of Alfalfa

    Institute of Scientific and Technical Information of China (English)

    张媚佳; 徐淑韵; 徐苏凌; 方勇; 徐根娣

    2011-01-01

    The seeding of alfalfa on the acid soil with acid-tolerant rhizobium is to investigate the effects on the growth of alfalfa, and provide a theoretical and technical basis for the cultivation of alfalfa to the south. The results show that acid-tolerant rhizobium inoculation has promoted the growth of the 2 tested alfalfas, and there is breed difference of alfalfa when establishing symbiosis relationship with rhizobium.%为给南方紫花苜蓿的种植提供一定的理论和技术依据,采用土培法在酸性土壤上接种耐酸根瘤茵以探讨其对紫花苜蓿生长情况的影响.结果表明,接种耐酸根瘤菌对参试的2种紫花苜蓿生长都有促进作用,且紫花苜蓿在与根瘤菌建立共生关系时存在品种差异.

  17. Dagens tilbud: Et pust til cv’et

    DEFF Research Database (Denmark)

    Meyer, Gitte

    2012-01-01

    ’Dear dr. Gitte Meyer’ lød indledningen forleden på det, der viste sig at være en art akademisk Nigeria-brev. Det var da underholdende. Men mailen er også et udtryk for, at hele publiceringscirkusset trænger til et eftersyn.......’Dear dr. Gitte Meyer’ lød indledningen forleden på det, der viste sig at være en art akademisk Nigeria-brev. Det var da underholdende. Men mailen er også et udtryk for, at hele publiceringscirkusset trænger til et eftersyn....

  18. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Bauer, Susanne

    2015-02-09

    We participated in a FASTER SCM intercomparison for which we ran our SCM for 3 years at the SGP to analyze statistics of the precipitation field (Song et al., 2013). An important feature of these simulations was the use of relaxation forcing to observed T, q, which decouples the model convection from the forcing and allows precipitation errors to emerge. Because the GISS cumulus parameterization includes a trigger that prevents convection until sufficient lifting is present, and because convection at the SGP is usually triggered by mesoscale motions that are not represented in the forcing when relaxation is applied, the duration of SCM precipitation is shorter than observed (Del Genio and Wolf, 2012) and thus its mean precipitation less than observed. However, its diurnal cycle phase is correct, and it is the only operational cumulus parameterization in the intercomparison that does not produce excessive warm season precipitation under weak large-scale forcing conditions.

  19. Flipped Learning fra et elevperspektiv

    DEFF Research Database (Denmark)

    Holmboe, Peter; Hachmann, Roland

    2016-01-01

    Antologi er et resultat af et toårigt udviklingsprojekt om Flipped Learning baseret på et samarbejde mellem IT-Center Fyn og Region Syddanmark. Bidraget her præsenterer en række overvejelser, vi har gjort os på baggrund af observationer af undervisning og interviews med elever i projektet. Vores...... overordnede undersøgelsesspørgsmål var: "Hvilke forhold i undervisning og læringsmiljø fremme hhv. begrænser elevens opfattelse af mening og fagligt udbytte i et Flipped Learning design?"....

  20. Musique et émotion

    OpenAIRE

    Ciarcia, Gaetano; DeNora, Tia; Jouvenet, Morgan; De L'Estoile, Benoît; Maisonneuve, Sophie; Martin, Denis-Constant; Paradis, Annie; Roueff, Olivier; Segré, Gabriel; Seraïdari, Katerina; Wateau, Fabienne

    2005-01-01

    Loin de relever par excellence d'un ineffable résistant à toute analyse, voire à toute description, la musique est le fruit de pratiques et d'usages riches et complexes. C'est pourquoi les articles réunis ici n'appréhendent pas " la musique " en tant que phénomène universel dont il faudrait décrire les illustrations locales mais mettent en avant ses utilisations et leurs effets réels et, notamment, le rapport privilégié des pratiques musicales aux émotions.

  1. Symbiotic effectiveness and ecological characterization of indigenous Rhizobium loti populations in Uruguay Eficiência simbiótica e caracterização ecológica de uma população nativa de Rhizobium loti no Uruguai

    Directory of Open Access Journals (Sweden)

    Amalia Baraibar

    1999-06-01

    Full Text Available The objectives of this work were to describe the distribution, density and seasonal variation of the indigenous populations of Rhizobium loti in different Uruguayan soils and to determine the symbiotic effectiveness and stress tolerance factors of different isolates, both with the aim of obtaining selected strains to re-introduce as inoculants in Lotus pastures. R. loti was present in ten soils studied and their densities varied from year to year and within each soil. All the isolates nodulated Lotus corniculatus effectively. The nodules in Lotus pedunculatus and Lotus subbiflorus were small, red on the surface and ineffective in nitrogen fixation. The study of 50 isolates from the ten soils showed high variability in their symbiotic efficiency and tolerance to pH. The indigenous population was acid tolerant in culture medium (pH 4.5, 83% of them could grow at pH 4.5 in 3 days. This work showed that there was a great diversity between the strains of R. loti isolated from Uruguayan soils and supports the importance of selecting among them the most efficient and resistant strains to be included in the inoculants.Os objetivos deste trabalho foram descrever a distribuição, densidade e variação sazonal de populações nativas de Rhizobium loti em diferentes solos uruguaios, e determinar a eficiência simbiótica e os fatores de tolerância a estresse de diversos isolados, tendo em vista a obtenção de cepas a serem reintroduzidas como inoculantes em pastagens de Lotus. Rhizobium loti estava presente em dez solos estudados, e suas densidades variavam de ano para ano e em cada solo. Todos os isolados nodularam efetivamente o Lotus corniculatus. Os nódulos no Lotus pedunculatus e no Lotus subbiflorus eram pequenos, vermelhos na superfície, e ineficazes na fixação de N. O estudo de 50 isolados dos dez solos mostraram alta variabilidade na sua eficiência simbiótica, e alta tolerância ao pH. A população nativa era tolerante à acidez em meio

  2. Produção de mudas de acácia colonizadas com micorrizas e rizóbio em diferentes recipientes Production of acacia plants colonized with mycorrhizas and rhizobium in different recipients

    Directory of Open Access Journals (Sweden)

    Jolimar Antonio Schiavo

    2003-02-01

    Full Text Available Realizou-se um experimento em casa de vegetação, com o objetivo de avaliar diferentes métodos na produção de mudas de Acacia mangium Willd, colonizadas com fungos micorrízicos arbusculares (FMAs e rizóbio. O delineamento experimental foi o inteiramente casualizado num esquema fatorial 4x2 (controle, FMAs, rizóbio e FMAs + rizóbio x blocos prensados e tubetes de plástico, com seis repetições. Os blocos prensados foram confeccionados com substratos orgânicos (bagaço de cana + torta de filtro de usina açucareira e vermiculita, colocados em fôrma metálica de 60x40x20 cm e prensados a 10 kgf cm-2, a fim de proporcionar agregação do material. A inoculação do rizóbio foi realizada com estirpe selecionada para a espécie (Br 3609, Br 6009. A inoculação de FMAs foi feita no momento da confecção dos blocos. Mudas de Acacia mangium que receberam inóculo de FMAs + rizóbio e produzidas em blocos prensados apresentaram maior produção de matéria seca e conteúdo de N na parte aérea. O conteúdo de P na parte aérea é significativamente maior somente nas mudas infectadas com os FMAs, independentemente do tipo de recipiente.A greenhouse experiment was carried out in order to evaluate different methods to produce Acacia mangium Willd plant seedlings, inoculated with arbuscular mycorrhizal fungi (AMF and rhizobium. A completely randomized design in a factorial scheme 4x2 (control, AMF, rhizobium and AMF + rhizobium x pressed blocks and plastic tubes, with six repetitions was used. The pressed blocks used to produce Acacia mangium plants were made with organic residue from sugarcane (sugarcane bagasse + filter cake and vermiculite. The inoculation with rhizobium was done with selected strain (Br 3609, Br 6009. The inoculation with AMF was done at the time when pressed blocks were made. Acacia mangium plants inoculated with both AMF + rhizobium led to a significant increase in dry matter yield and N content of shoot plants, only in

  3. Le vieillard et le chevalier

    Directory of Open Access Journals (Sweden)

    Mélanie JECKER

    2010-05-01

    Full Text Available La fin du Moyen Âge fascine. Deux facteurs principaux expliquent l’attraction qu’exerce cette période : elle apparaît d’une part comme un moment historique riche car double, phase de transition entre le Moyen Âge et la Renaissance, où se mêlent des cadres de représentation anciens et nouveaux. L’insinuation d’une pensée humaniste balbutiante s’y traduirait notamment par l’émergence de la notion d’individu, qui constitue l’un des objets de ce travail. Il faut invoquer, d’autre part, l’image macabre d’un bas Moyen Âge hanté par l’idée de la maladie et de la mort depuis l’épidémie de peste noire de 1348, obsédé par la brièveté de la vie humaine. Ces deux facettes de « l’automne du Moyen Âge » tel que l’évoque avec poésie Joan Huizinga sont-elles autre chose qu’une plaisante image d’Epinal? Cette étude cherche précisément à déterminer le degré de validité de ces deux topiques, à travers l’analyse de la représentation des âges de la vie dans un corpus bien défini, celui de la biographie chevaleresque castillane du XVe siècle. L’intérêt porté à l’individu, la conscience de la singularité impliquent en effet celle du caractère mouvant de la personne, or quel thème mieux que celui des âges permet de confirmer ou d’infirmer l’hypothèse du surgissement d’une nouvelle manière de concevoir l’homme, proprement « renaissante », en ce siècle de transition , La nature même du corpus retenu – des récits biographiques – suggère en effet une mutation dans l’appréhension de la personne, une attention prêtée à sa progressive transformation. L’étude de la représentation de la vieillesse, en particulier, révèle non seulement à quel point était alors réelle cette prétendue obsession de la déchéance physique et de la mortalité, mais aussi dans quelle mesure étaient soulignés et valorisés les changements individuels parallèles à l

  4. Culture et droit processuel en Belgique

    OpenAIRE

    de Leval, Georges; Georges, Frédéric

    2010-01-01

    Cet ouvrage regroupe le rapport de synthèse, les rapports généraux et les rapports nationaux relatifs aux quatre thèmes suivants : culture et droit civil, culture et droit international, culture et droit processuel et culture et droit des affaires

  5. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    R Paul Drake

    2004-01-12

    OAK-B135 This is the final report from the project Hydrodynamics by High-Energy-Density Plasma Flow and Hydrodynamics and Radiation Hydrodynamics with Astrophysical Applications. This project supported a group at the University of Michigan in the invention, design, performance, and analysis of experiments using high-energy-density research facilities. The experiments explored compressible nonlinear hydrodynamics, in particular at decelerating interfaces, and the radiation hydrodynamics of strong shock waves. It has application to supernovae, astrophysical jets, shock-cloud interactions, and radiative shock waves.

  6. ET come home

    DEFF Research Database (Denmark)

    Fisher, J. B.; Whittaker, R. J.; Malhi, Y.

    2011-01-01

    Aim Many macroecological analyses are based on analyses of climatological data, within which evapotranspiration estimates are of central importance. In this paper we evaluate and review the use of evapotranspiration models and data in studies of geographical ecology to test the likely sensitivity...... evapotranspiration, outline basic evapotranspiration models and describe methods with which to measure evapotranspiration. Next, we compare three different types of potential evapotranspiration models – a temperature-based (Thornthwaite type), a radiation-based (Priestley–Taylor) and a combination (Penman......–Monteith) model – for 11 different land-cover types. Finally, we compare these models at continental and global scales. Results At some sites the models differ by less than 7%, but generally the difference was greater than 25% across most sites. The temperature-based model estimated 20–30% less than the radiation...

  7. Et håndtryk

    DEFF Research Database (Denmark)

    Eriksen, Karen Westphal; Schytz Juul, Mathilde

    2010-01-01

    Hør Karen Westphal Eriksen fortælle om udvalgte værker og temaer fra udstillingen "Et håndtryk". Udstillingen omhandler dansk-fransk avantgardekunst og er et samarbejde mellem Fyns Kunstmuseum og Statens Museum for Kunst. Udstillingen kan ses på Fyns Kunstmuseum frem til februar 2011...

  8. Temps et musique en Chine

    OpenAIRE

    Picard, François

    2008-01-01

    The practice of time in music is related to the people's conception of time. The case of China.; La pratique des rythmes et des articulations en musique s'articule avec le temps vécu et conçu par les gens. Vu de la Chine.

  9. Effects of high temperature on survival, symbiotic performance and genomic modifications of bean nodulating Rhizobium strains Sobrevivência, fixação de nitrogênio e modificações genéticas em estirpes de Rhizobium sp. efetivas na nodulação do feijoeiro, expostas à altas temperaturas

    Directory of Open Access Journals (Sweden)

    Patrícia P. Pinto

    1998-10-01

    Full Text Available High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. In this study R. tropici and R. leguminosarum bv. phaseoli strains isolated from Cerrado soils were exposed to thermal stress and the strains’ growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analyzed. After successive thermal shocks at 45ºC for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici strains. Certain R. leguminosarum bv. phaseoli strains had significant alterations in plant dry weight and DNA patterns obtained by AP-PCR method. R. tropici strains (with the exception of FJ2.21 were more stable than R. leguminosarum bv. phaseoli strains because no significant phenotypic alterations were observed following thermal treatments and they maintained their original genotypic pattern after inoculation in plants.Altas temperaturas podem afetar a sobrevivência, estabelecimento e as propriedades simbióticas em estirpes de Rhizobium. As estirpes capazes de nodular o feijoeiro têm sido consideradas particularmente sensíveis, porque nessas estirpes é comum a ocorrência de recombinações e/ou deleções genômicas comprometendo, muitas vezes, a sua utilização como inoculantes. Neste trabalho, procurou-se avaliar a capacidade de crescimento e sobrevivência em temperaturas elevadas de estirpes de Rhizobium efetivas na fixação de nitrogênio no feijoeiro isoladas dos cerrados, bem como avaliar suas características fenotípicas e genotípicas após choque térmico. A capacidade de sobrevivência à temperaturas elevadas, avaliada após choques térmicos sucessivos (45ºC por 4

  10. Analyse de Langues et cultures - Les TIC, enseignement et apprentissage

    Directory of Open Access Journals (Sweden)

    Élisabeth Brodin

    2006-04-01

    Full Text Available 1. Introduction Cet ouvrage constitue les actes du colloque des IUFM (Institut Universitaire de Formation des Maîtres de décembre 2003, intitulé "Quelles nouvelles perspectives pour l'enseignement / apprentissage des langues et cultures ?" On peut déplorer a priori que le titre du livre, au demeurant ambitieux, et le sous-titre ne reprennent pas la question des nouvelles perspectives apportées par les Technologies de l'Information et de la Communication (TIC à l'enseignement des langues e...

  11. Investing EU ETS auction revenues into energy savings

    Energy Technology Data Exchange (ETDEWEB)

    Sijm, J.P.M.; Boonekamp, P.G.M. [ECN Policy Studies, Petten (Netherlands); Summerton, P.; Pollitt, H.; Billington, S. [Cambridge Econometrics CE, Cambridge (United Kingdom)

    2013-05-15

    The overall objective of this study is to analyse the effects of using EU ETS auction revenues to stimulate investments in energy savings in three key target sectors, i.e. Households, Tertiary and Industry (including both ETS and non-ETS industrial installations). The scenarios used refer basically to the situation before the recent agreement on the Energy Efficiency Directive (EED) and include (a mixture of) different policy options to enhance energy savings in the target sectors, in particular (1) reducing the ETS cap, (2) introducing an Energy Efficiency Obligation (EEO) for energy suppliers or distributors, and/or (3) using ETS auction revenues to support additional (private) investments in raising energy efficiency. In order to meet this objective a variety of different policy scenarios have been defined and analysed by means of the 'Energy-Environment-Economy Model for Europe (E3ME)'. The study presents and discusses a large variety of scenario modelling results by the year 2020 at the EU27 level. These results refer to, among others, energy savings, GHG emissions, the ETS carbon price, household electricity bills and to changes in some macro- or socio-economic outcomes such as GDP, inflation, employment or international trade. Finally, the study discusses some policy findings and implications, including options to enhance the effectiveness of some EE policies, in particular those having a potential adverse effect on the ETS carbon price.

  12. Rhizobium leguminosarum COMO ORGANISMO BIOCONTROLADOR DE LA INTERACCIÓN HOSPEDERO-PATÓGENO: CLAVEL (Dianthus caryophyllus – Fusarium Oxysporum f. sp. dianthi

    Directory of Open Access Journals (Sweden)

    Cheol Woo Lee Park

    2010-08-01

    Full Text Available Se investigó el efecto de biocontrol de Rhizobium leguminosarum (R. leguminosarum cepa B, contra Fusarium oxysporum f.sp.dianthi (FOD raza 2, en la interacción Clavel - FOD. Se utilizó la raza 2 de FOD por ser la de mayor patogenicidad y distribución en las fincas de cultivo de clavel en Colombia. Para ello se establecieron las condiciones de inoculación de FOD sobre cultivos establecidos de R . leguminosarum, variando la concentración in vitro de la bacteria. Se encontró una reducción en el número de microconidias hasta un 90% y una inhibición en el crecimiento radial del patógeno de hasta un 71 %. En el ensayo de microcultivo dual se detectó fraccionamiento de las hifas después de 48 horas de incubación con R . leguminosarum En el ensayo in vivo, los esquejes de clavel Raggio di solé, variedad susceptible al patógeno, fueron inoculados con 45.0 x 10 células de R. leguminosarum por matero, mostrando una severidad inferior al 5%, una incidencia menor del 20% y una reducción del índice de la enfermedad hasta de un 92% en presencia del patógeno.

  13. Formulation of microbes inoculum: AMF, PSB and Rhizobium isolated of ex-coal mining site for Acacia crassicarpa Cunn. Ex-benth seedlings

    Directory of Open Access Journals (Sweden)

    ENNY WIDYATI

    2007-07-01

    Full Text Available The shoddier succeed land revegetation particularly caused by least adaptability of the seedlings planted on this site. To encourage their growth and survival rate it can be achieved by means do inoculation with the compatible functional microbes such as rhizobium, Psolubilizing bacteria (PSB and/or arbuscular-mycorrhiza fungy (AMF. This reserach is aimed to formulate the most compatible inoculant to support the growth of A. crassicarpa seedlings. Compatibility study is carried out in RCB design with 3 replications, each contain 5 seedlings. Height and biomass are accessed to measure the growth responses of the seedlings. The result showed that the best reponse is given by consortia that consist of the three kinds of these microbes. This increase the shoot biomass (137% compare to the control. The consortia also improved N 164%, P 335% and K 167% in the plant tissues. While pure AMF improved absorption of N plants 80%, P 383% and K 51% compare to the control. It is suggested that to prepare the A. crassicarpa seedlings is better inoculated by consortium of microbes or AMF as a sole inoculant.

  14. The Aquaeous Extract of Root Nodules Vigna radiata (rnVr which Inoculated by Rhizobium as an Orally Available Anemia Therapeutic Candidate

    Directory of Open Access Journals (Sweden)

    Dewi Hidayati

    2015-11-01

    Full Text Available The extract of root nodules Vigna radiata (rnVr which inoculated by Rhizobium is considered beneficial as an orally available anemia therapeutic candidate, because it contain the leghemoglobin. The positive control mice (group I were fed with the high nutrient pellet.The twelve mice (Mus musculus was treated with the “taking rice pellet” that representing the low nutrient food for 21 days until they suffered anemia. Then, the anemia mice were treated orally with rnVr in different concentration groups:II. 0% III.33%; IV.67% and V.100%, respectively and fed with the “aking rice pellet”. After 14 days, the blood mice were collected from orbital sinus. The hemoglobin (Hb concentration were analyzed by spectrophotometry and blood plasma profile protein were analyzed with electrophoresis (SDS-PAGE. All anemia mice that treated with rnVr showed the increasing of Hb and group that treated with 100% extract of rnVr could reach a normal Hb value, raising from 9.85 to 12.68 g/dL. There were observed the proteins which have molecule weight 36.5 and 35.7 kDa that indicated the existing erythropoietin. The increasing haemoglobin concentration and erythropoietin suggested if extract of rnVr could increasing red blood production and potential as an orally available anemia therapeutic candidate.

  15. Production and Metabolism of Indole Acetic Acid in Root Nodules and Symbiont (Rhizobium undicola Isolated from Root Nodule of Aquatic Medicinal Legume Neptunia oleracea Lour.

    Directory of Open Access Journals (Sweden)

    Pallab Kumar Ghosh

    2015-01-01

    Full Text Available Indole acetic acid is a phytohormone which plays a vital role in plant growth and development. The purpose of this study was to shed some light on the production of IAA in roots, nodules, and symbionts of an aquatic legume Neptunia oleracea and its possible role in nodular symbiosis. The symbiont (N37 was isolated from nodules of this plant and identified as Rhizobium undicola based on biochemical characteristics, 16S rDNA sequence homology, and DNA-DNA hybridization results. The root nodules were found to contain more IAA and tryptophan than root; however, no detectable amount of IAA was found in root. The IAA metabolizing enzymes IAA oxidase, IAA peroxidase (E.C.1.11.1.7, and polyphenol oxidase (E.C.1.14.18.1 were higher in root than nodule but total phenol and IAA content were reversed. The strain N37 was found to produce copious amount of IAA in YEM broth medium with tryptophan and reached its stationary phase at 20 h. An enrichment of the medium with mannitol, ammonium sulphate, B12, and 4-hydroxybenzaldehyde was found to promote the IAA production. The presence of IAA metabolizing enzymes and IAA production with PGPR traits including ACC deaminase activity of the symbionts was essential for plant microbe interaction and nodule function.

  16. Bacillus simplex—A Little Known PGPB with Anti-Fungal Activity—Alters Pea Legume Root Architecture and Nodule Morphology When Coinoculated with Rhizobium leguminosarum bv. viciae

    Directory of Open Access Journals (Sweden)

    Ann M. Hirsch

    2013-09-01

    Full Text Available Two strains, 30N-5 and 30VD-1, identified as Bacillus simplex and B. subtilis, were isolated from the rhizospheres of two different plants, a Podocarpus and a palm, respectively, growing in the University of California, Los Angeles (UCLA Mildred E. Mathias Botanical Garden. B. subtilis is a well-known plant-growth promoting bacterial species, but B. simplex is not. B. simplex 30N-5 was initially isolated on a nitrogen-free medium, but no evidence for nitrogen fixation was found. Nevertheless, pea plants inoculated with B. simplex showed a change in root architecture due to the emergence of more lateral roots. When Pisum sativum carrying a DR5::GUSA construct, an indicator for auxin response, was inoculated with either B. simplex 30N-5 or its symbiont Rhizobium leguminosarum bv. viciae 128C53, GUS expression in the roots was increased over the uninoculated controls. Moreover, when pea roots were coinoculated with either B. simplex 30N-5 or B. subtilis 30VD-1 and R. leguminosarum bv. viciae 128C53, the nodules were larger, clustered, and developed more highly branched vascular bundles. Besides producing siderophores and solubilizing phosphate, the two Bacillus spp., especially strain 30VD-1, exhibited anti-fungal activity towards Fusarium. Our data show that combining nodulating, nitrogen-fixing rhizobia with growth-promoting bacteria enhances plant development and strongly supports a coinoculation strategy to improve nitrogen fixation, increase biomass, and establish greater resistance to fungal disease.

  17. Rhizobium leguminosarum bv. viciae 3841 Adapts to 2,4-Dichlorophenoxyacetic Acid with "Auxin-Like" Morphological Changes, Cell Envelope Remodeling and Upregulation of Central Metabolic Pathways.

    Directory of Open Access Journals (Sweden)

    Supriya V Bhat

    Full Text Available There is a growing need to characterize the effects of environmental stressors at the molecular level on model organisms with the ever increasing number and variety of anthropogenic chemical pollutants. The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D, as one of the most widely applied pesticides in the world, is one such example. This herbicide is known to have non-targeted undesirable effects on humans, animals and soil microbes, but specific molecular targets at sublethal levels are unknown. In this study, we have used Rhizobium leguminosarum bv. viciae 3841 (Rlv as a nitrogen fixing, beneficial model soil organism to characterize the effects of 2,4-D. Using metabolomics and advanced microscopy we determined specific target pathways in the Rlv metabolic network and consequent changes to its phenotype, surface ultrastructure, and physical properties during sublethal 2,4-D exposure. Auxin and 2,4-D, its structural analogue, showed common morphological changes in vitro which were similar to bacteroids isolated from plant nodules, implying that these changes are related to bacteroid differentiation required for nitrogen fixation. Rlv showed remarkable adaptation capabilities in response to the herbicide, with changes to integral pathways of cellular metabolism and the potential to assimilate 2,4-D with consequent changes to its physical and structural properties. This study identifies biomarkers of 2,4-D in Rlv and offers valuable insights into the mode-of-action of 2,4-D in soil bacteria.

  18. 13N-nitrate uptake sites and rhizobium-infectible region in a single root of common bean [Phaseolus vulgaris] and soybean

    International Nuclear Information System (INIS)

    The positron emitting tracer-imaging system (PETIS) was used to determine whether it was possible to obtain on image of 13N distribution in common bean in which a single root was fed with a liquid medium containing nitrate at different concentrations with different 13N specific activities. The distribution of the images of the 13N atoms in the root could be obtained over a wide range of nitrate concentrations and 13N specific activities in the medium. As for nitrate stress on leguminous root nodulation, the positional relationship between the nitrate uptake sites and root hair just elongating area, where rhizobia capably initiate their infection, was studied in common bean and soybean. PETIS gave direct evidence that single roots of both common bean and soybean showed one or two dense 13N-distribution areas after 2 min pulse-feeding of 13NO3(-). These areas remained stable over 30 min, and the first dense site, which was common in all the examined roots, extended over ca. 1 cm above the root apex. Microscopic observation revealed that this area covered both sites of rhizobium infection and of early nodule development in a common bean and soybean single root

  19. Intelligence Naturelle et Intelligence Artificielle

    OpenAIRE

    DuBois, Daniel

    2011-01-01

    Cet article présente une approche systémique du concept d’intelligence naturelle en ayant pour objectif de créer une intelligence artificielle. Ainsi, l’intelligence naturelle, humaine et animale non-humaine, est une fonction composée de facultés permettant de connaître et de comprendre. De plus, l'intelligence naturelle reste indissociable de la structure, à savoir les organes du cerveau et du corps. La tentation est grande de doter les systèmes informatiques d’une intelligence artificielle ...

  20. Ressources humaines et territoires ruraux

    OpenAIRE

    Dauty, Françoise; Tahar, Gabriel; Vernières, Michel

    2007-01-01

    Note Lirhe 440 (07-3) L'hypothèse centrale de ce travail collectif est que la dotation en ressources naturelles et même en capital physique des territoires ruraux n'apparaît pas comme le premier ressort de leur développement. Celui-ci est essentiellement fonction de leur capacité à innover socialement et économiquement, à mobiliser les ressources humaines dont ils disposent et à en attirer de nouvelles. L'impulsion initiale peut être issue d'entrepreneurs, d'associations ou d'autorités pol...

  1. Espace et musique en Chine

    OpenAIRE

    Picard, François

    1998-01-01

    The notions of high and low are not such important in the Chinese way of making and writing music. The place, the space and the spatial relations are on the opposite quite important. Plutôt qu'explorer les notions de bas et de haut, qui n'occupent pas une place importante dans la conception ou la pratique chinoises de la musique, l'espace en tant que lieu établissant des relations entre musiciens et entre musiciens et auditeurs est examiné à travers diverses situations

  2. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Webb, Robert C. [Texas A& M University; Kamon, Teruki [Texas A& M University; Toback, David [Texas A& M University; Safonov, Alexei [Texas A& M University; Dutta, Bhaskar [Texas A& M University; Dimitri, Nanopoulos [Texas A& M University; Pope, Christopher [Texas A& M University; White, James [Texas A& M University

    2013-11-18

    Overview The High Energy Physics Group at Texas A&M University is submitting this final report for our grant number DE-FG02-95ER40917. This grant has supported our wide range of research activities for over a decade. The reports contained here summarize the latest work done by our research team. Task A (Collider Physics Program): CMS & CDF Profs. T. Kamon, A. Safonov, and D. Toback co-lead the Texas A&M (TAMU) collider program focusing on CDF and CMS experiments. Task D: Particle Physics Theory Our particle physics theory task is the combined effort of Profs. B. Dutta, D. Nanopoulos, and C. Pope. Task E (Underground Physics): LUX & NEXT Profs. R. Webb and J. White(deceased) lead the Xenon-based underground research program consisting of two main thrusts: the first, participation in the LUX two-phase xenon dark matter search experiment and the second, detector R&D primarily aimed at developing future detectors for underground physics (e.g. NEXT and LZ).

  3. Épices et condiments

    OpenAIRE

    M. Gast

    2012-01-01

    Les Berbères, traditionnellement grands consommateurs de lait et de viande, n’ont pas de plat spécifique qui donne lieu à des préparations élaborées faisant appel à des produits exotiques ; le couscous, les galettes, les bouillies ne donnent pas lieu à des recherches de goût et de saveur renouvelés, du moins chez les populations à régime économique pauvre. La faim endémique qui a sévi au cours des siècles parmi les populations nomades et rurales du Maghreb et du Sahara, n’a guère favorisé l’e...

  4. Consoer et al PFOS dataset

    Data.gov (United States)

    U.S. Environmental Protection Agency — This ScienceHub entry was developed for the published paper: Consoer et al., 2016, Toxicokinetics of perfluorooctane sulfonate in rainow trout (Oncorhynchus...

  5. Seltzer_et_al_2016

    Data.gov (United States)

    U.S. Environmental Protection Agency — This dataset supports the modeling study of Seltzer et al. (2016) published in Atmospheric Environment. In this study, techniques typically used for future air...

  6.  Final review of the Séralini et al. (2012a publication on a 2-year rodent feeding study with glyphosate formulations and GM maize NK603 as published online on 19 September 2012 in Food and Chemical Toxicology

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2012-11-01

    Full Text Available On 19 September 2012, Séralini et al. published online in the scientific journal Food and Chemical Toxicology a publication describing a 2-year feeding study in rats investigating the health effects of genetically modified maize NK603 with and without Roundup WeatherMAX® and Roundup® GT Plus alone (both are glyphosate-containing plant protection products. As requested by the European Commission, EFSA reviewed this publication taking into consideration assessments conducted by Member States and any clarification given by the authors. The assessments of Member States and EFSA revealed an overall agreement. The study as reported by Séralini et al. was found to be inadequately designed, analysed and reported. The authors of Séralini et al. provided a limited amount of relevant additional information in their answer to critics published in the journal Food and Chemical Toxicology. Taking into consideration Member States’ assessments and the authors’ answer to critics, EFSA reaches similar conclusions as in its first Statement (EFSA 2012. The study as described by Séralini et al. does not allow giving weight to their results and conclusions as published. Conclusions cannot be drawn on the difference in tumour incidence between treatment groups on the basis of the design, the analysis and the results as reported. Taking into consideration Member States’ assessments and the authors’ answer to critics, EFSA finds that the study as reported by Séralini et al. is of insufficient scientific quality for safety assessments. EFSA concludes that the currently available evidence does not impact on the ongoing re-evaluation of glyphosate and does not call for the reopening of the safety evaluations of maize NK603 and its related stacks. EFSA’s evaluation of the Séralini et al. article is in keeping with its role to review relevant scientific literature for risk assessment on an ongoing basis to ensure that the advice it provides is up-to-date.

  7. Porokeratosis palmaris et plantaris disseminata

    Directory of Open Access Journals (Sweden)

    Kaur Vineet

    1993-01-01

    Full Text Available A 30-year-old woman had porokeratosis palmaris et plantaris disseminata with unusual features. These included the sex of the patient, absence of family history of similar disease, onset of lesions on the shins and a significant furrow. There was also associated diabetes mellitus. It is suggested that porokeratosis palmaris et plantaris disseminata falls in the middle of the spectrum of porokeratoses.

  8. Et forslag til religionsneutral begravelsesplads

    DEFF Research Database (Denmark)

    Tvedt, Tilde; Lund, Signe

    2014-01-01

    Flere og flere mennesker ønsker sig et alternativ til den traditionelle kristne kirkegård. Enten fordi de ikke er religiøse, eller fordi de tilhører andre religioner. Et speciale på Københavns Universitet ser på, hvordan de nye behov kan imødekommes med en religionsneutral begravelsesplads i tæt...

  9. At the sidelines of implementing the EU ETS: objections to ‘validity’

    OpenAIRE

    Truxal, S.

    2010-01-01

    As the European Union's Emissions Trading Scheme (EU ETS) nears the final stages of implementation across Member States, it earns greater global recognition. An investigation into the ethos and status of the EU ETS raises interesting spaces to lead a sideline discussion on actual and likely objections by industries which emit greenhouse gases. It would appear that although the EU ETS is a model clean development mechanism under the Kyoto Protocol to the United Nations Framework Convention on ...

  10. Définition des réseaux de surveillance DCE de la qualité des masses d’eau côtières de l’île de Mayotte - Rapport final Tome 1 Synthèse et propositions

    OpenAIRE

    Turquet, Jean; Nicet, Jean Benoit; Cambert, Harold; Denis, Yves; Gonzalez, Jean-Louis; Bigot, Lionel; Guyomarch, Julien; Budzinski, Helene; Tapie, Nathalie; Jamon, Alban; Pribat, B

    2010-01-01

    Les prochaines échéances imposées par le calendrier de la Directive Cadre sur l’Eau sur les eaux côtières visent à atteindre ou à restaurer le bon état qualitatif et quantitatif de toutes les masses d’eau d'ici l'horizon 2015. Ces objectifs nécessitent la définition du bon état chimique des masses d’eaux, l’évaluation des tendances et la mise en place des programmes de mesures.

  11. An overview of current research on EU ETS: Evidence from its operating mechanism and economic effect

    International Nuclear Information System (INIS)

    The European Union Emissions Trading Scheme (EU ETS) is supposed to be an important mechanism for addressing climate change. Up to now, the theoretical foundation of EU ETS has been widely acknowledged, but empirical research on its current situation has only been published recently or is forthcoming. Therefore, this paper is aimed to summarize the main arguments of empirical studies on the EU ETS, in terms of two aspects, i.e., the operating mechanism and economic effect of the EU ETS, which are two crucial topics and have been attached much attention. Based on the shortcomings of current research and future requirements of the EU ETS evolution, finally, we also present some further directions of the EU ETS research. Overall, the research overview here may be helpful to recognize the features of the EU ETS and its effect on others. (author)

  12. Validación técnica de inoculantes en frijol con cepas de rhizobium eficientes en fijación de nitrógeno en Centroamérica

    OpenAIRE

    Oscar Acu\\u00F1a; Emigdio Rodr\\u00EDguez; Aurelio Llano; Vilma Ruth Calder\\u00F3n; Guillermo Flores; Abelardo Viana; Rogelio L\\u00E9piz

    2001-01-01

    Se realizó un ensayo de validación técnica de inoculantes en frijol elaborado con cepas de Rhizobium eficientes en fijación biológica de nitrógeno, con el objetivo de recabar mayor información sobre la bondad de esta tecnología en la producción comercial de frijol. El ensayo regional se sembró en 45 sitios, distribuidos en Costa Rica (20), Panamá (10), Nicaragua (10) y El Salvador (5), durante 1998 y 1999. Se incluyeron cuatro tratamientos: 1. Testigo absoluto; 2. Inoc...

  13. Validación técnica de inoculantes en frijol con cepas de rhizobium eficientes en fijación de nitrógeno en Centroamérica

    OpenAIRE

    Oscar Acuña; Emigdio Rodríguez; Aurelio Llano; Vilma Ruth Calderón; Guillermo Flores; Abelardo Viana; Rogelio Lépiz

    2001-01-01

    Se realizó un ensayo de validación técnica de inoculantes en frijol elaborado con cepas de Rhizobium eficientes en fijación biológica de nitrógeno, con el objetivo de recabar mayor información sobre la bondad de esta tecnología en la producción comercial de frijol. El ensayo regional se sembró en 45 sitios, distribuidos en Costa Rica (20), Panamá (10), Nicaragua (10) y El Salvador (5), durante 1998 y 1999. Se incluyeron cuatro tratamientos: 1. Testigo absoluto; 2. Inoculado; 3. Fertilización ...

  14. Vêtement et textile : sources et ressources

    Directory of Open Access Journals (Sweden)

    Sophie Jolivet-Jacquet

    2009-09-01

    Full Text Available Progressivement, les acteurs de la recherche scientifique s’organisent en réseaux, conscients que l’amélioration des connaissances passe par le partage et l’élaboration collective. L’outil internet offre une interface efficace, mais son offre est si multiple qu’elle mérite d’être organisée. L’histoire du vêtement, des matières et des accessoires textiles bénéficie désormais d’une interface spécifique à l’échelle de la recherche française, mais aussi internationale. Le projet « Vêtement et tex...

  15. Diffusion et utilisation des TIC en France et en Europe

    OpenAIRE

    Berret, Pierre; Chantepie, Philippe

    2015-01-01

    L’exploitation par le DEPS des enquêtes communautaires sur l’utilisation des TIC par les ménages et les particuliers, coordonnées, harmonisées et publiées par Eurostat, permet de dresser un portrait comparatif et en tendances de l’équipement en TIC, des modes d’accès à l’internet des ménages dans l’UE- 27 selon leurs caractéristiques socio-démographiques. Elle met en lumière l’intensification des usages numériques des particuliers, élucide les facteurs de développement des TIC que sont les us...

  16. Plafonds froids et introduction d'air laminaire

    CERN Document Server

    Pepinster, P

    1998-01-01

    Dès le milieu des années 1980, on a pu observer une tendance à l'augmentation des charges thermiques internes dans les bâtiments administratifs, principalement due à l'arrivée de l'informatique et de la bureautique. Cet accroissement de charges a finalement atteint un record de 100 W/m2 fin des années 80 pour se stabiliser en règle générale entre 50 et 70 W/m2. Traditionnellement, c'est le renouvellement d'air refroidi et insufflé dans les locaux qui combat les apports calorifiques, et le débit d'air pulsé est proportionnel aux charges à évacuer. Cette augmentation des charges a donc généré des vitesses de circulation d'air résiduelles insatisfaisantes pour le confort des occupants, et les ingénieurs en climatisation ont dû revoir la technologie appliquée jusqu'alors. Ce document présente les techniques actuelles qui permettent de satisfaire à l'exigence d'absence de courant d'air aux places de travail, et ainsi assurer le bien-être humain dans le domaine climatique.

  17. Approche et traitement des cybercrises

    Directory of Open Access Journals (Sweden)

    Bourdeau Elodie

    2000-09-01

    Full Text Available Avec l’explosion d’Internet, de nombreuses entreprises du secteur agro-alimentaire sont aujourd’hui amenées à faire face à des crises d’un genre nouveau qui voient le jour sur Internet, et qu’on appelle cybercrises. Pour les y aider, la société Startem, créée en 1994 et regroupant une soixantaine de consultants, propose aux entreprises de ce secteur des moyens afin de gérer au mieux ces crises. Ainsi, parmi d’autres activités, Startem recueille et analyse tout ce qui se dit ou s’écrit dans les médias sur chacun de ses clients, et ce dans 95 pays et 23 langues. De plus, Startem propose d’accompagner ses clients dans la gestion d’une crise en aidant, par exemple, à la mise en place de sites Internet/Intranet de crise.

  18. Entre blogues et web diaries

    OpenAIRE

    Lang, Marie-Ève

    2013-01-01

    Dans le cadre d’une recherche doctorale portant sur la façon dont les adolescentes et les jeunes femmes âgées de 17 à 21 ans utilisent le Web pour s’informer sur la sexualité, l’auteure présente la méthode qualitative qu’elle a élaborée et qui est basée sur l’activité du carnetage (blogging). Elle discute ici des caractéristiques particulières et des avantages d’une telle méthode. Drawing from her doctoral project on the uses of the Internet by teenage girls and young women between the age...

  19. Agrochimie, Suisse et Tiers Monde

    OpenAIRE

    Delucchi, Vittorio

    2013-01-01

    1. Situation générale Les deux catégories de produits chimiques les plus utilisées en agriculture sont les engrais et les pesticides [définition : voir HAYES, 1991]. La Suisse en a exporté en 1990 pour une valeur d’env. 796 millions de francs et importé pour une valeur d’env. 230 millions [ANONYME, 1991]. Le commerce extérieur de ces produits agrochimiques s’est surtout développé avec les pays de la Communauté Européenne (CE) et de l’Association Européenne de libre Echange (AELE), toutefois d...

  20. Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL domain effector of Rhizobium sp. strain NGR234.

    Directory of Open Access Journals (Sweden)

    Da-Wei Xin

    Full Text Available Type 3 effector proteins secreted via the bacterial type 3 secretion system (T3SS are not only virulence factors of pathogenic bacteria, but also influence symbiotic interactions between nitrogen-fixing nodule bacteria (rhizobia and leguminous host plants. In this study, we characterized NopM (nodulation outer protein M of Rhizobium sp. strain NGR234, which shows sequence similarities with novel E3 ubiquitin ligase (NEL domain effectors from the human pathogens Shigella flexneri and Salomonella enterica. NopM expressed in Escherichia coli, but not the non-functional mutant protein NopM-C338A, showed E3 ubiquitin ligase activity in vitro. In vivo, NopM, but not inactive NopM-C338A, promoted nodulation of the host plant Lablab purpureus by NGR234. When NopM was expressed in yeast, it inhibited mating pheromone signaling, a mitogen-activated protein (MAP kinase pathway. When expressed in the plant Nicotiana benthamiana, NopM inhibited one part of the plant's defense response, as shown by a reduced production of reactive oxygen species (ROS in response to the flagellin peptide flg22, whereas it stimulated another part, namely the induction of defense genes. In summary, our data indicate the potential for NopM as a functional NEL domain E3 ubiquitin ligase. Our findings that NopM dampened the flg22-induced ROS burst in N. benthamiana but promoted defense gene induction are consistent with the concept that pattern-triggered immunity is split in two separate signaling branches, one leading to ROS production and the other to defense gene induction.

  1. Rhizobium leguminosarum symbiovar trifolii, Ensifer numidicus and Mesorhizobium amorphae symbiovar ciceri (or Mesorhizobium loti) are new endosymbiotic bacteria of Lens culinaris Medik.

    Science.gov (United States)

    Sami, Dhaoui; Mokhtar, Rejili; Peter, Mergaert; Mohamed, Mars

    2016-08-01

    A total of 142 rhizobial bacteria were isolated from root nodules of Lens culinaris Medik endemic to Tunisia and they belonged to the species Rhizobium leguminosarum, and for the first time to Ensifer and Mesorhizobium, genera never previously described as microsymbionts of lentil. Phenotypically, our results indicate that L. culinaris Medik strains showed heterogenic responses to the different phenotypic features and they effectively nodulated their original host. Based on the concatenation of the 16S rRNA with relevant housekeeping genes (glnA, recA, dnaK), rhizobia that nodulate lentil belonged almost exclusively to the known R. leguminosarum sv. viciae. Interestingly, R. leguminosarum sv. trifolii, Ensifer numidicus (10 isolates) and Mesorhizobium amorphae (or M. loti) (9 isolates) isolates species, not considered, up to now, as a natural symbiont of lentil are reported. The E. numidicus and M. amorphae (or M. loti) strains induced fixing nodules on Medicago sativa and Cicer arietinum host plants, respectively. Symbiotic gene phylogenies showed that the E. numidicus, new symbiont of lentil, markedly diverged from strains of R. leguminosarum, the usual symbionts of lentil, and converged to the symbiovar meliloti so far described within E. meliloti Indeed, the nodC and nodA genes from the M. amorphae showed more than 99% similarity with respect to those from M. mediterraneum, the common chickpea nodulating species, and would be included in the new infrasubspecific division named M. amorphae symbiovar ciceri, or to M. loti, related to the strains able to effectively nodulate C. arietinum host plant. On the basis of these data, R. leguminosarum sv. trifolii (type strain LBg3 (T)), M. loti or M. amorphae sv. ciceri (type strain LB4 (T)) and E. numidicus (type strain LBi2 (T)) are proposed as new symbionts of L. culinaris Medik. PMID:27267929

  2. Functional analysis of the cysteine motifs in the ferredoxin-like protein FdxN of Rhizobium meliloti involved in symbiotic nitrogen fixation.

    Science.gov (United States)

    Masepohl, B; Kutsche, M; Riedel, K U; Schmehl, M; Klipp, W; Pühler, A

    1992-05-01

    The Rhizobium meliloti fdxN gene, which is part of the nifA-nifB-fdxN operon, is absolutely required for symbiotic nitrogen fixation. The deduced sequence of the FdxN protein is characterized by two cysteine motifs typical of bacterial-type ferredoxins. The Fix-phenotype of an R. meliloti fdxN::[Tc] mutant could be rescued by the R. leguminosarum fdxN gene, whereas no complementation was observed with nif-associated genes encoding ferredoxins from Bradyrhizobium japonicum, Azotobacter vinelandii, A. chroococcum and Rhodobacter capsulatus. In addition to these heterologous genes, several R. meliloti fdxN mutant genes constructed by site-directed mutagenesis were analyzed. Not only a cysteine residue within the second cysteine motif (position 42), which is known to coordinate the Fe-S cluster in homologous proteins, but also a cysteine located down-stream of this motif (position 61), was found to be essential for the activity of the R. meliloti FdxN protein. Changing the amino acid residue proline in position 56 into methionine resulted in a FdxN mutant protein with decreased activity, whereas changes in positions 35 (Asp35Glu) and 45 (Gly45Glu) had no significant effect on the function of the FdxN mutant proteins. In contrast to bacterial-type ferredoxins, which contain two identical cysteine motifs of the form C-X2-C-X2-C-X3-C, nif-associated ferredoxins, including R. meliloti FdxN, are characterized by two different cysteine motifs. Six "additional" amino acids separate the second (Cys42) and the third cysteine (Cys51) in the C-terminal motif (C-X2-C-X8-C-X3-C).(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1603075

  3. Unexpectedly Diverse Mesorhizobium Strains and Rhizobium leguminosarum Nodulate Native Legume Genera of New Zealand, while Introduced Legume Weeds Are Nodulated by Bradyrhizobium Species

    Science.gov (United States)

    Weir, Bevan S.; Turner, Susan J.; Silvester, Warwick B.; Park, Duck-Chul; Young, John M.

    2004-01-01

    The New Zealand native legume flora are represented by four genera, Sophora, Carmichaelia, Clianthus, and Montigena. The adventive flora of New Zealand contains several legume species introduced in the 19th century and now established as serious invasive weeds. Until now, nothing has been reported on the identification of the associated rhizobia of native or introduced legumes in New Zealand. The success of the introduced species may be due, at least in part, to the nature of their rhizobial symbioses. This study set out to address this issue by identifying rhizobial strains isolated from species of the four native legume genera and from the introduced weeds: Acacia spp. (wattles), Cytisus scoparius (broom), and Ulex europaeus (gorse). The identities of the isolates and their relationship to known rhizobia were established by comparative analysis of 16S ribosomal DNA, atpD, glnII, and recA gene sequences. Maximum-likelihood analysis of the resultant data partitioned the bacteria into three genera. Most isolates from native legumes aligned with the genus Mesorhizobium, either as members of named species or as putative novel species. The widespread distribution of strains from individual native legume genera across Mesorhizobium spp. contrasts with previous reports implying that bacterial species are specific to limited numbers of legume genera. In addition, four isolates were identified as Rhizobium leguminosarum. In contrast, all sequences from isolates from introduced weeds aligned with Bradyrhizobium species but formed clusters distinct from existing named species. These results show that native legume genera and these introduced legume genera do not have the same rhizobial populations. PMID:15466541

  4. Screening a wide host-range, waste-water metagenomic library in tryptophan auxotrophs of Rhizobium leguminosarum and of Escherichia coli reveals different classes of cloned trp genes.

    Science.gov (United States)

    Li, Youguo; Wexler, Margaret; Richardson, David J; Bond, Philip L; Johnston, Andrew W B

    2005-12-01

    A metagenomic cosmid library was constructed, in which the insert DNA was derived from bacteria in a waste-water treatment plant and the vector was the wide host-range cosmid pLAFR3. The library was screened for clones that could correct defined tryptophan auxotrophs of the alpha-proteobacterium Rhizobium leguminosarum and of Escherichia coli. A total of 26 different cosmids that corrected at least one trp mutant in one or both of these species were obtained. Several cosmids corrected the auxotrophy of one or more R. leguminosarum trp mutants, but not the corresponding mutants in E. coli. Conversely, one cosmid corrected trpA, B, C, D and E mutants of E. coli but none of the trp mutants of R. leguminosarum. Two of the Trp+ cosmids were examined in more detail. One contained a trp operon that resembled that of the pathogen Chlamydophila caviae, containing the unusual kynU gene, which specifies kynureninase. The other, whose trp genes functioned in R. leguminosarum but not in E. coli, contained trpDCFBA in an operon that is likely co-transcribed with five other genes, most of which had no known link with tryptophan synthesis. The sequences of these TRP proteins, and the products of nine other genes encoded by this cosmid, failed to affiliate them with any known bacterial lineage. For one metagenomic cosmid, lac reporter fusions confirmed that its cloned trp genes were transcribed in R. leguminosarum, but not in E. coli. Thus, rhizobia, with their many sigma-factors, may be well-suited hosts for metagenomic libraries, cloned in wide host-range vectors. PMID:16309391

  5. TtsI, a key regulator of Rhizobium species NGR234 is required for type III-dependent protein secretion and synthesis of rhamnose-rich polysaccharides.

    Science.gov (United States)

    Marie, Corinne; Deakin, William J; Ojanen-Reuhs, Tuula; Diallo, Ericka; Reuhs, Brad; Broughton, William J; Perret, Xavier

    2004-09-01

    Formation of nitrogen-fixing nodules on legume roots by Rhizobium sp. NGR234 requires an array of bacterial factors, including nodulation outer proteins (Nops) secreted through a type III secretion system (TTSS). Secretion of Nops is abolished upon inactivation of ttsI (formerly y4xI), a protein with characteristics of two-component response regulators that was predicted to activate transcription of TTSS-related genes. During the symbiotic interaction, the phenotype of NGR omega ttsI differs from that of a mutant with a nonfunctional secretion machine, however. This indicated that TtsI regulates the synthesis of other symbiotic factors as well. Conserved sequences, called tts boxes, proposed to act as binding sites for TtsI, were identified not only within the TTSS cluster but also in the promoter regions of i) genes predicted to encode homologs of virulence factors secreted by pathogenic bacteria, ii) loci involved in the synthesis of a rhamnose-rich component (rhamnan) of the lipopolysaccharides (LPS), and iii) open reading frames that play roles in plasmid partitioning. Transcription studies showed that TtsI and tts boxes are required for the activation of TTSS-related genes and those involved in rhamnose synthesis. Furthermore, extraction of polysaccharides revealed that inactivation of ttsI abolishes the synthesis of the rhamnan component of the LPS. The phenotypes of mutants impaired in TTSS-dependent protein secretion, rhamnan synthesis, or in both functions were compared to assess the roles of some of the TtsI-controlled factors during symbiosis.

  6. Roman historique et Histoire dans le roman

    Directory of Open Access Journals (Sweden)

    Christine Di Benedetto

    2008-12-01

    Full Text Available Engager une réflexion sur le « roman historique » dans l’Espagne de l’après-transition démocratique conduit ici à aborder les questions, plus vastes, d’Histoire dans le roman et de roman de la mémoire. Mais les notions de passé et de présent et leurs rapports se définissent au regard de la situation particulière de l’Espagne. L’interprétation de la place qu’occupe le roman historique dans le panorama romanesque espagnol s’enrichit alors des perspectives issues de la philosophie et de la sociologie.Au confluent de ces démarches, des exemples tirés de la littérature contemporaine et étayés par des éléments d’analyse fictionnelle et narrative figurent trois niveaux d’intégration de l’Histoire récente dans le roman espagnol. Et une identité en devenir se dessine, alors que la mémoire historique arrive clairement sur la scène littéraire en même temps qu’elle s’insinue dans les problématiques qui occupent la société espagnole de la fin du millénaire.Adentrarse en una reflexión sobre “la novela histórica” en la España de la post- transición democrática lleva aquí a acercarse a los temas, más amplios, de Historia en la novela y de novela de la memoria. Pero las nociones de pasado y de presente se definen en el ámbito de la situación peculiar de España, y de allí el vínculo cualitativo entre las dos. La interpretación del puesto que ocupa en la narrativa española se enriquece entonces de las perspectivas que proceden de la filosofía y de la sociología.En el confluente de estas modalidades reflexivas, unos ejemplos, sacados de la literatura contemporánea y estudiados gracias a elementos de análisis de la ficción y de la narración destacan tres niveles de integración de la Historia reciente en la novela española. De modo que una búsqueda identitaria se define, mientras la memoria histórica llega claramente al escenario literario, al mismo tiempo que se cuela dentro de las

  7. Dannelsens korridor. Et argument for et udvidet dannelsesbegreb

    DEFF Research Database (Denmark)

    Smedegaard Ernst Bengtsen, Søren

    til et eksistentielt fænomen, hvor det dannede individ risikerer sin egen viden, destabiliserer det kulturelle paradigme, han tilhører, samt stræber efter mærkelige, sære og bekymrende vidensformer. Denne artikel argumenterer for, at ingen af disse to modsatrettede dannelsesbegreber kan stå alene, men...

  8. Hvid er et usynligt ideal

    DEFF Research Database (Denmark)

    Mehlsen, Camilla

    2010-01-01

    Salget af hudblegecremer er eksploderet. Hvorfor drømmer så mange om at få lys hud? Hvorfor vil børn hellere lege med hvide dukker end brune? Svaret er, at hvid er et globalt ideal. Interview med Dorthe Staunæs og Les Back.......Salget af hudblegecremer er eksploderet. Hvorfor drømmer så mange om at få lys hud? Hvorfor vil børn hellere lege med hvide dukker end brune? Svaret er, at hvid er et globalt ideal. Interview med Dorthe Staunæs og Les Back....

  9. Orientation entrepreneuriale : racines et bourgeons.

    OpenAIRE

    Randerson, Kathleen,

    2012-01-01

    L’objectif de cette thèse est d’apporter de nouveaux éclairages théoriques et empiriques sur les mécanismes d’entrepreneuriat organisationnel, et plus précisément « orientation entrepreneuriale » (OE). Afin de mieux comprendre ce phénomène, cette dissertation est le fruit de quatre efforts successifs : - Identifier ce qu’est l’orientation entrepreneuriale en le distinguant de ce que ce n’est pas - Comprendre comment le construit « OE » s’intègre dans les principaux modèles d’entrepreneuriat o...

  10. la progesterone et la grossesse

    OpenAIRE

    HAMDANT, .DJ; DOUCH, .F; OUAKKA, .A; TABET DERREZ, .I; .BOUARICHA, .N

    2010-01-01

    Les progestatifs au cours de la grossesse restent largement indiquer pour prévenir ou traiter une menace d'avortement spontané soit la prévention et traitement des menaces d'accouchement prématuré. D'après les resultats de notre travail, on a constaté que les effets de la progestérone sont minimes sur la durée de grossesse et la modalité d'accouchement. La prescription de progestérone dans un but thérapeutique durant la grossesse garde sa place en montrant son effecaci...

  11. Le soutien communautaire et religieux

    OpenAIRE

    Hocquelet, Mathieu

    2013-01-01

    Description générale du contenu : 24 avril 2013, 8 heures du matin, parking du magasin Walmart de Lakewood Blvd, Paramount, Californie, en périphérie de Los Angeles.Action menée par l’organisation Our Walmart et ses alliés locaux (curés, associations progressistes). Selon les tracts distribués aux clients, il s’agit de soutenir les employés de l’entreprise luttant pour obtenir du management davantage de respect, faire cesser les réductions de personnel et les menaces de représailles. Commenta...

  12. Le droit et les femmes

    OpenAIRE

    Collin, Françoise; Duroux, Françoise; Rochefort, Florence

    2014-01-01

    Quelques dates 1804 Le code civil napoléonien efface les quelques dispositions prises en 1792 sur le droit au divorce et restaure l’incapacité civile des femmes mariées. 1886 Rétablissement du droit au divorce. 1907 Les femmes mariées peuvent percevoir leur salaire. 1937 Trois femmes sont nommées sous-secrétaires d’État, sans droit de vote. 1944 Droit de vote pour les femmes. 1965 Droit d’exercer une profession et d’ouvrir un compte bancaire sans l’autorisation du mari. 1970 L’autorité parent...

  13. ETS rearrangements in prostate cancer

    Institute of Scientific and Technical Information of China (English)

    Mark A Rubin

    2012-01-01

    Prostate cancer is a clinically and molecularly heterogeneous disease.Understanding the biologic underpinning of prostate cancer is necessary to best determine how biology is associated with the risk of disease progression and how this understanding might provide insight into the development of novel therapeutic approaches.The focus of this review is on the recently identified common ETS and non-ETS gene rearrangements in prostate cancer.Although multiple molecular alterations have been detected in prostate cancer,a basic understanding of gene fusion prostate cancer should help explain the clinical and biologic diversity,providing a rationale for a molecular subclassification of the disease.

  14. Georges et le big bang

    CERN Document Server

    Hawking, Lucy; Parsons, Gary

    2011-01-01

    Georges et Annie, sa meilleure amie, sont sur le point d'assister à l'une des plus importantes expériences scientifiques de tous les temps : explorer les premiers instants de l'Univers, le Big Bang ! Grâce à Cosmos, leur super ordinateur, et au Grand Collisionneur de hadrons créé par Éric, le père d'Annie, ils vont enfin pouvoir répondre à cette question essentielle : pourquoi existons nous ? Mais Georges et Annie découvrent qu'un complot diabolique se trame. Pire, c'est toute la recherche scientifique qui est en péril ! Entraîné dans d'incroyables aventures, Georges ira jusqu'aux confins de la galaxie pour sauver ses amis...Une plongée passionnante au coeur du Big Bang. Les toutes dernières théories de Stephen Hawking et des plus grands scientifiques actuels.

  15. Le funambule et le parergon

    Directory of Open Access Journals (Sweden)

    Xavier Jeudon

    2015-09-01

    Full Text Available Loin de la fascination qu’exerce la chute, promesse de renouveau pour Baudelaire et de plaisir pour Adorno, le funambule fait quotidiennement l’expérience d’une prise et d’une maîtrise du risque. Faisant du risque un spectacle, le funambule évolue sur un fil, nous renvoyant aux premières sensations de la marche, mal assurée, hésitante et tellement audacieuse. Le funambule travaillant au quotidien son équilibre se doit donc de prendre des risques, mais aussi de donner l’illusion d’une première fois : simuler le danger là où il est pourtant. C’est en jouant sur le moment de la rupture, de la chute que le funambule capte l’attention. Il donne un corps à cette question de la limite et semble nous proposer une adaptation douce au risque, là où le dompteur parait s’y opposer plus frontalement.

  16. Et skæg

    DEFF Research Database (Denmark)

    Rytter, Mikkel

    2014-01-01

    I islam er profeten Muhammad en refleksion af Guds vilje, og han har følgelig status som “det perfekte menneske’. Profeten er således et ideal og eksempel til efterfølgelse for muslimer verden over. Dette kapitel diskuterer, hvordan danske sufi-muslimer an- lægger sig signifikante fuldskæg – selvom...

  17. Batailles professionnelles et enjeux politiques

    Directory of Open Access Journals (Sweden)

    Éric Gobe

    2014-05-01

    Full Text Available Les batailles territoriales que se sont livrées experts comptables et avocats sous le régime de Ben Ali montrent que, dans un contexte autoritaire, les logiques professionnelles et celles du politique sont intimement imbriquées. L’État tunisien a arbitré entre différents intérêts professionnels en fonction des nécessités de la pérennisation du régime autoritaire. Les rapports de force politique entre les différents acteurs, i.e. les gouvernants, le barreau, les experts comptables et les autres professions du chiffre, ont contribué au découpage d’un territoire professionnel perçu comme défavorable par des avocats eux-mêmes appréhendés par le pouvoir comme une profession dangereuse. La « révolution » tunisienne de 2011 a inversé provisoirement les rapports de force en faveur du barreau qui a su tirer parti de la chute du président Ben Ali pour faire aboutir ses revendications et réasseoir son pouvoir professionnel.

  18. Et pourquoi pas au CERN ?

    CERN Multimedia

    Staff Association

    2015-01-01

    Télétravail ou travail à distance, aménagement des horaires de travail et autres évolutions favorables à un meilleur équilibre vie privée et vie professionnelle sont adoptés par nombre d’entreprises et d’organisations !   Rendu possible grâce au développement de nouvelles technologies dont Internet, le travail à distance séduit de plus en plus de personnels, ainsi que de plus en plus de sociétés qui y trouvent des avantages en matière de gestion de l’espace, de sécurité (moins de trajets domicile-entreprise), de développement durable (moins de pollution), de motivation et de bien-être de leurs personnels. Les horaires aménagés, voire les « core-hours1 », sont également des pratiques de plus e...

  19. Figures mythiques et histoire. Réinterprétations et contrastes entre Orient et Occident

    Directory of Open Access Journals (Sweden)

    Denise Aigle

    2009-05-01

    Full Text Available L'histoire de l'Orient musulman est jalonnée de grands personnages qui servent de modèles de référence et d'ancêtres fondateurs, certains hérités de l'islam, d'autres de l'Antiquité et de la Bible. Leur figure s'est transformée en Orient musulman à partir du Xe siècle, largement sous l'influence du Shâh-nâma, quand se sont multipliées les dynasties indépendantes, puis à la suite de l'irruption des Turcs, des Croisés et des Mongols. Les exemples de Saladin et de Gengis Khan illustrent le renouvellement des modèles, leur extension et leurs réinterprétations successives. La créativité mythique apparaît comme un acte culturel, de portée éminemment politique. La carrière d'un grand conquérant comme Tamerlan a servi de modèle à ses épigones pour justifier leurs prétentions territoriales. Les références bibliques attestées dans le Coran ont permis d'intégrer les Turcs, puis les Mongols, dans l'histoire de la Création, comme descendants de l'un des trois fils de Noé. Des rattachements bibliques anciens ont valorisé les musulmans des régions de la Volga à partir du XVIIIe siècle, ainsi que les différents peuples chrétiens et musulmans du Caucase, région riche en références bibliques comme l'échouage de l'arche de Noé sur le mont Ararat et l'enfermement des peuples de Gog et Magog derrière des portes de fer.

  20. Droit, administration publique et TIC en Tunisie

    OpenAIRE

    Ben Letaief, Mustapha

    2014-01-01

    Le développement accéléré des technologies de l’information et de la communication (Tic) dans le monde a suscité et continue de susciter l’intérêt de nombreux acteurs : décideurs, managers publics et privés, entreprises, responsables administratifs, citoyens et usagers. Il ne peut donc laisser indifférents le droit et ses acteurs : législateur, pouvoir réglementaire, juges, doctrine, administration et Citoyens. Droit constitutionnel et droit administratif devaient réagir. Dans le contexte tun...

  1. Micorriza arbuscular e rizóbios no enraizamento e nutrição de mudas de angico-vermelho Arbuscular mycorrhizae and rhizobium in rooting and nutrition of angico-vermelho seedlings

    Directory of Open Access Journals (Sweden)

    Poliana Coqueiro Dias

    2012-12-01

    Full Text Available O objetivo deste estudo foi avaliar o efeito da inoculação dos fungos micorrízicos arbusculares (FMAs e rizóbio no enraizamento, crescimento e nutrição de mudas de angico-vermelho (Anadenanthera macrocarpa (Benth Brenan propagadas via miniestaquia. Foram utilizadas seis progênies, das quais foram confeccionadas miniestacas com um par de folhas inteiras, bem como tubetes de 55 cm³ contendo substrato comercial Bioplant®. Foram testados quatro tratamentos: 8 kg m-3 de superfosfato simples (SS misturados ao substrato; 4 kg m-3 de SS misturados ao substrato; 4 kg m-3 de SS misturados ao substrato e adição de suspensão contendo rizóbios; e 4 kg m-3 de SS e adição de suspensão contendo rizóbios e 5 g de solo contendo esporos de FMAs. Não houve interação entre os tratamentos para percentagem de sobrevivência das miniestacas e percentagem de miniestacas com raízes observadas na extremidade inferior do tubete, na saída da casa de vegetação (30 dias e da casa de sombra (40 dias, provavelmente em função do sistema radicular ainda estar em formação. Houve diferenças entre as progênies para percentagem de sobrevivência das miniestacas, percentagem de miniestacas com raízes observadas na extremidade inferior do tubete, altura, diâmetro de colo e massa seca da parte aérea. As avaliações das características de crescimento das miniestacas enraizadas, principalmente com relação à sobrevivência a pleno sol (140 dias, evidenciam a eficiência dos rizóbios e FMAs na produção de mudas desta espécie. Conclui-se que a associação simbiótica com rizóbio e/ou FMA favorece a produção de mudas de A. macrocarpa via miniestaquia.The objective of this study was to evaluate the effect of inoculation of arbuscular mycorrhizae fungi (AMF and rhizobium on rooting, growth and nutrition of seedlings of angico-red (Anadenanthera macrocarpa (Benth Brenan propagated by minicutting. Six progenies were used, of which were prepared

  2. Territoire et population de 1800 à 1890

    OpenAIRE

    2011-01-01

    Les noms des fichiers de données commencent par le préfixe TERR. Fichier Labels Territoire et Population TERR_T85.xls Territoire et Population, 1824-1837 (Départements) TERR_T86.xls Territoire et Population, 1801-1836 (Départements) TERR_T116.xls Territoire et Population, 1801-1836 (Chefs-lieux et villes) TERR_T117.xls Territoire, Population, 1800-1890 (Arrondissements)

  3. Antiquaires et antiquités chez Gillray et Daumier

    OpenAIRE

    Contogouris, Ersy

    2014-01-01

    Dans La Parade d’Honoré Daumier [Fig.1], cinq saltimbanques tentent ans par tous les moyens d’attirer l’attention du public : déguisés, ils s’interpellent pellent et gesticulent, l’un pointe vers la scène burlesque d’un crocodile ans par tous les moyens d’attirer l’attention du public : déguisés, ils s’interpellent et gesticulent, l’un pointe vers la scène burlesque d’un crocodile pourchassant sa prochaine victime, tandis que l’autre fait entrevoir ce qu’il y a derrière le rideau pour éveille...

  4. Konflikter i et inkluderende perspektiv

    DEFF Research Database (Denmark)

    Togsverd, Line; Ørskov, Keld

    2010-01-01

    I artiklen søger vi at give et bidrag til en faglig viden og analytisk tilgang, der kan bruges i refleksionen over hvordan børn gives deltagelsesmuligheder i børnehavens hverdagsliv, aktiviteter og fællesskaber. Konkret sætter vi fokus på et hverdagsfænomen som de fleste vil kende til, nemlig kon...... konflikter blandt børn, og uoverensstemmelser mellem børn og pædagoger. Situationer fra hverdagens samspil fortælles, analyseres og fortolkes med henblik på at overveje, hvad der kan tænkes at fremme eller hæmme børns deltagelsesmuligheder....

  5. INCERTITUDE ET ENVIRONNEMENT : EVALUATIONS ECONOMIQUES

    OpenAIRE

    Robert Kast

    2007-01-01

    Parmi les nombreux problèmes que posent la prise en compte de l’Environnement en économie et plus précisément dans l’étude des risques, le rôle, la nature et les représentations de l’incertitude sont fondamentaux. Que ce soit les dangers que provoquent leur environnement aux activités humaines, ou le souci de celles-ci pour l’Environnement1, il s’agit pour les humains de prendre des décisions risquées dans les deux cas. Risques naturels, notamment si la nature est ressentie comme ennemie, ris...

  6. Les jeux et les concours

    OpenAIRE

    Colignon, Jean-Pierre; Cunow, Yves; Hardoüin, Hervé; L’hénoret, Liliane

    2016-01-01

    La 5e Dictée des Lyriades Le samedi 29 mai à Liré, de nombreux candidats se sont penchés sur le texte, truffé de pièges, de la 5e Dictée des Lyriades, « Un stylo habile », composée, lue et animée par Jean-Pierre Colignon, membre des Lyriades, responsable du concours national de dictée et animateur de la dictée de Paris. Un stylo habile… Du coin de l’œil, le Ligérien Anatole, un ex-quincaillier, surveillait la neuchâteloise du salon… Il lui fallait patienter encore trois quarts d’heure avant d...

  7. Finances et politique en Bretagne

    OpenAIRE

    Le Page, Dominique; Kerhervé, Jean

    2013-01-01

    De 1491 date du mariage de Charles VIII et d’Anne de Bretagne à 1547 fin du règne de François Ier la Bretagne vit une étape importante de son histoire qui la voit passer du statut de principauté quasi autonome à celui de province intégrée au royaume. De cette évolution on a gardé le souvenir de l’édit d’Union de 1532, qui a défini les relations entre la Bretagne et la France jusqu'à la Révolution de 1789. L’étude de la politique menée par la monarchie dans le domaine financier au cours de la ...

  8. Tradition et écriture

    OpenAIRE

    Boutry, Philippe

    2013-01-01

    Le concept de tradition, tel qu’il est aujourd’hui communément utilisé par les spécialistes des sciences sociales, n’est nullement étranger, par son histoire et par son contenu, à la notion de tradition telle que l’ont élaborée depuis deux millénaires les théologiens ; et la réflexion des seconds peut, sous certains rapports, concourir aux questionnements des premiers. Des origines du christianisme aux définitions du concile de Trente (1546) sur « les deux sources de la révélation » qui fixen...

  9. Post Mortem et Tony Manero

    OpenAIRE

    Urrutia, Carolina

    2015-01-01

    Post Mortem, troisième long-métrage de Pablo Larraín, pourrait être le prélude du film précédent, Tony Manero ; le moment où le protagoniste (joué dans les deux cas par Alfredo Castro) tombe dans la folie sans retour et devient psychopathe. Le pari des deux films est extrême, car il assume la représentation et la relecture d’une époque extrême, où la démence mise en action sous le régime militaire semble être déjà installée dans la société qui lui donne naissance. Cette folie ne fait plus qu’...

  10. Langage et surdité

    OpenAIRE

    Bahan, Benjamin; Blondel, Marion; Cuxac, Christian; Lee, Robert G.; MacLaughlin, Dawn; Miller, Christopher; Morford, Jill P.; Neidle, Carol; Tuller, Laurice

    2005-01-01

    Les manifestations langagières liées à la surdité constituent des cas de langage « extraordinaires » et donc une source potentielle d'informations précieuses sur la nature du langage humain. Ce numéro présente un état des lieux des recherches actuelles dans le domaine « langage et surdité » : y sont traitées la structure formelle des langues des signes naturelles, mais aussi les spécificités de l'expression poétique dans ces langues, leurs caractéristiques lorsqu'elles sont acquises tardiveme...

  11. Georges et le code secret

    CERN Document Server

    Hawking, Lucy; Parsons, Gary

    2015-01-01

    À Foxbridge, Georges et Annie s'ennuient, ils rêvent de repartir dans l'espace pour de nouvelles aventures intergalactiques. Pourtant, sur la terre ferme, il se passe des choses étranges... L'argent s'envole des distributeurs de billets, les avions refusent de décoller et les caisses enregistreuses des supermarchés ne fonctionnent plus... Quel chaos ! Il semblerait qu'un bug planétaire ait déréglé tous les systèmes informatiques. S'ils veulent découvrir ce qui se cache derrière tout ça, les deux amis devront voyager plus loin que jamais dans l'espace...

  12. Basisindkomst som et transkulturelt perspektiv

    DEFF Research Database (Denmark)

    Ydesen, Christian; Christensen, Erik

    2013-01-01

    Tanken om en ubetinget basisindkomst til alle, der hidtil især har været præget af en vestlig økonomisk tænkning, er i sammenhæng med globalisering og den verdensøkonomiske krise i stigende grad blevet set som et element i en kriseløsning; ikke kun i den vestlige verden. Faktisk er forskellige pr...

  13. "Et ..." : [luuletused] / Kalle Istvan Eller

    Index Scriptorium Estoniae

    Eller, Kalle Istvan, pseud., 1940-

    2002-01-01

    Sisu: "Et ..." ; "päivämõõk lahkse päivä ..." ; "yy kirves lei mant ..." ; kuus om lõhmuse ..." ; "mägi om sattul ..." ; "obetsemägi ..." ; "härtmavitsake ..." ; "Taivast paistus veel ..." ; "soe' lätsi orgo pite ..." ; Pääsoke ; "ilm jääss ütsinda ...". Eluloolisi andmeid autori kohta lk. 366

  14. Mente et manu / Heili Sõrmus

    Index Scriptorium Estoniae

    Sõrmus, Heili

    2012-01-01

    Eesti Kunstiakadeemia magistritaseme lõputööde näitus "Tase", ehte- ja sepakunsti bakalaureusetaseme lõpetajate näitus "Kiiremini! Kaugemale! Kõrgemale! ehk K", ehtekunsti II kursuse tudengite näitus "Etüüdid", klaasikunsti osakonna näitus "Face book", klaasikunstnike Kristiina Oppi, Mai Schultsi ja Darja Popolitova näitus "... on vaataja silmades"

  15. Max Planck et les quanta

    CERN Document Server

    Boudenot, Jean-Claude

    2016-01-01

    « Les atomes, dit Jean Perrin en 1913, ne sont pas ces éléments éternels et insécables dont l'irréductible simplicité donnait au possible une borne, et, dans leur inimaginable petitesse, nous commençons à pressentir un fourmillement prodigieux de mondes nouveaux ». C'est bien dans un monde totalement nouveau, le monde quantique, que nous a fait pénétrer la découverte des quanta par Max Planck. Son article de 1900 est le déclencheur de l'une des plus grandes révolutions scientifiques de tous les temps. Les trente années qui suivent sont les plus riches de la physique ; Planck, Einstein, Bohr, Sommerfeld, de Broglie, Schrödinger, Heisenberg, Dirac, Born, Pauli… reconstruisent la physique sur de nouvelles bases sur fond de conflit des générations. Le monde est par ailleurs secoué par la guerre, Max Planck est tourmenté et vit des épreuves personnelles dramatiques. C'est l'homme, aussi bien que l'oeuvre, que les auteurs ont tenté de dépeindre dans cet ouvrage. Ils ont également souhait�...

  16. Crystallization and X-ray diffraction analysis of an l-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a d-xylonate dehydratase from Caulobacter crescentus

    Science.gov (United States)

    Rahman, Mohammad Mubinur; Andberg, Martina; Koivula, Anu; Rouvinen, Juha; Hakulinen, Nina

    2016-01-01

    l-Arabinonate dehydratase (EC 4.2.1.25) and d-xylonate dehydratase (EC 4.2.1.82) are two enzymes that are involved in a nonphosphorylative oxidation pathway of pentose sugars. l-Arabinonate dehydratase converts l-arabinonate into 2-dehydro-3-deoxy-l-arabinonate, and d-xylonate dehydratase catalyzes the dehydration of d-xylonate to 2-dehydro-3-deoxy-d-xylonate. l-Arabinonate and d-xylonate dehydratases belong to the IlvD/EDD family, together with 6-phosphogluconate dehydratases and dihydroxyacid dehydratases. No crystal structure of any l-arabinonate or d-xylonate dehydratase is available in the PDB. In this study, recombinant l-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii (RlArDHT) and d-xylonate dehydratase from Caulobacter crescentus (CcXyDHT) were heterologously expressed in Escherichia coli and purified by the use of affinity chromatography followed by gel-filtration chromatography. The purified proteins were crystallized using the hanging-drop vapour-diffusion method at 293 K. Crystals of RlArDHT that diffracted to 2.40 Å resolution were obtained using sodium formate as a precipitating agent. They belonged to space group P21, with unit-cell parameters a = 106.07, b = 208.61, c = 147.09 Å, β = 90.43°. Eight RlArDHT molecules (two tetramers) in the asymmetric unit give a V M value of 3.2 Å3 Da−1 and a solvent content of 62%. Crystals of CcXyDHT that diffracted to 2.66 Å resolution were obtained using sodium formate and polyethylene glycol 3350. They belonged to space group C2, with unit-cell parameters a = 270.42, b = 236.13, c = 65.17 Å, β = 97.38°. Four CcXyDHT molecules (a tetramer) in the asymmetric unit give a V M value of 4.0 Å3 Da−1 and a solvent content of 69%. PMID:27487924

  17. Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus.

    Science.gov (United States)

    Rahman, Mohammad Mubinur; Andberg, Martina; Koivula, Anu; Rouvinen, Juha; Hakulinen, Nina

    2016-08-01

    L-Arabinonate dehydratase (EC 4.2.1.25) and D-xylonate dehydratase (EC 4.2.1.82) are two enzymes that are involved in a nonphosphorylative oxidation pathway of pentose sugars. L-Arabinonate dehydratase converts L-arabinonate into 2-dehydro-3-deoxy-L-arabinonate, and D-xylonate dehydratase catalyzes the dehydration of D-xylonate to 2-dehydro-3-deoxy-D-xylonate. L-Arabinonate and D-xylonate dehydratases belong to the IlvD/EDD family, together with 6-phosphogluconate dehydratases and dihydroxyacid dehydratases. No crystal structure of any L-arabinonate or D-xylonate dehydratase is available in the PDB. In this study, recombinant L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii (RlArDHT) and D-xylonate dehydratase from Caulobacter crescentus (CcXyDHT) were heterologously expressed in Escherichia coli and purified by the use of affinity chromatography followed by gel-filtration chromatography. The purified proteins were crystallized using the hanging-drop vapour-diffusion method at 293 K. Crystals of RlArDHT that diffracted to 2.40 Å resolution were obtained using sodium formate as a precipitating agent. They belonged to space group P21, with unit-cell parameters a = 106.07, b = 208.61, c = 147.09 Å, β = 90.43°. Eight RlArDHT molecules (two tetramers) in the asymmetric unit give a VM value of 3.2 Å(3) Da(-1) and a solvent content of 62%. Crystals of CcXyDHT that diffracted to 2.66 Å resolution were obtained using sodium formate and polyethylene glycol 3350. They belonged to space group C2, with unit-cell parameters a = 270.42, b = 236.13, c = 65.17 Å, β = 97.38°. Four CcXyDHT molecules (a tetramer) in the asymmetric unit give a VM value of 4.0 Å(3) Da(-1) and a solvent content of 69%.

  18. A mannosyl transferase required for lipopolysaccharide inner core assembly in Rhizobium leguminosarum. Purification, substrate specificity, and expression in Salmonella waaC mutants.

    Science.gov (United States)

    Kanipes, Margaret I; Ribeiro, Anthony A; Lin, Shanhua; Cotter, Robert J; Raetz, Christian R H

    2003-05-01

    The lipopolysaccharide (LPS) core domain of Gram-negative bacteria plays an important role in outer membrane stability and host interactions. Little is known about the biochemical properties of the glycosyltransferases that assemble the LPS core. We now report the purification and characterization of the Rhizobium leguminosarum mannosyl transferase LpcC, which adds a mannose unit to the inner 3-deoxy-d-manno-octulosonic acid (Kdo) moiety of the LPS precursor, Kdo(2)-lipid IV(A). LpcC containing an N-terminal His(6) tag was assayed using GDP-mannose as the donor and Kdo(2)-[4'-(32)P]lipid IV(A) as the acceptor and was purified to near homogeneity. Sequencing of the N terminus confirmed that the purified enzyme is the lpcC gene product. Mild acid hydrolysis of the glycolipid generated in vitro by pure LpcC showed that the mannosylation occurs on the inner Kdo residue of Kdo(2)-[4'-(32)P]lipid IV(A). A lipid acceptor substrate containing two Kdo moieties is required by LpcC, since no activity is seen with lipid IV(A) or Kdo-lipid IV(A). The purified enzyme can use GDP-mannose or, to a lesser extent, ADP-mannose (both of which have the alpha-anomeric configuration) for the glycosylation of Kdo(2)-[4'-(32)P]lipid IV(A). Little or no activity is seen with ADP-glucose, UDP-glucose, UDP-GlcNAc, or UDP-galactose. A Salmonella typhimurium waaC mutant, which lacks the enzyme for incorporating the inner l-glycero-d-manno-heptose moiety of LPS, regains LPS with O-antigen when complemented with lpcC. An Escherichia coli heptose-less waaC-waaF deletion mutant expressing the R. leguminosarum lpcC gene likewise generates a hybrid LPS species consisting of Kdo(2)-lipid A plus a single mannose residue. Our results demonstrate that heterologous lpcC expression can be used to modify the structure of the Salmonella and E. coli LPS cores in living cells.

  19. Optimización de las condiciones de fermentación para la producción de polihidroxibutirato por Rhizobium tropici

    Directory of Open Access Journals (Sweden)

    Yuliet Franco

    2009-01-01

    Full Text Available Los polihidroxialcanoatos (PHAs, son poliésteres acumulados intracelularmente como materiales de reserva de carbono y energía por varios microorganismos. El representante más común de esta familia es el poli-b-hidroxibutirato (PHB. Estos biopolímeros se consideran buenos sustitutos de los plásticos sintéticos derivados del petróleo por ser biodregadrables. Sin embargo, la mayor desventaja para la comercialización de los PHA son sus elevados costos de producción comparados con los plásticos derivados del petróleo. La optimización del proceso de fermentación, es una de las estrategias más utilizadas para reducir estos costos. Este trabajo tuvo como objetivos determinar el valor óptimo de pH inicial de la fermentación para la producción de PHB, así como optimizar las condiciones de agitación y aeración del proceso para el rendimiento producto biomasa y para la acumulación de PHB por la cepa Rhizobium tropici 3. La optimización del pH inicial se realizó en zaranda orbital ajustando el valor entre 6 y 8 y se modeló la relación entre el pH y la concentración de PHB (g · L-1 mediante la utilización de polinomios ortogonales. Se definió que el pH inicial óptimo con el cual se asegura la mayor producción de PHB es 6,58 ± 0,2. La búsqueda de los valores óptimos de agitación-aeración para la producción de PHB y para el rendimiento producto-biomasa, se realizó mediante el empleo de un diseño central compuesto Box-Wilson. Las fermentaciones se realizaron en un fermentador de 7,5 L con 5 L de medio de cultivo optimizado. Se determinó que los valores óptimos para la producción fueron 500 r · min-1 y 1 vvm (volumen de aire por minuto por volumen de medio de cultivo para la agitación y la aeración respectivamente y para el rendimiento producto-biomasa, los valores óptimos fueron 472 r · min-1 y 1,55 vvm.

  20. La ville et la cour

    Directory of Open Access Journals (Sweden)

    Jean-Pierre BARRAQUÉ

    2009-07-01

    Full Text Available Saragosse, capitale affirmée de l’Aragon, souffre au XVe siècle de l’absence de ses rois. Aussi les rares séjours des souverains constituent-ils de véritables événements, d’autant qu’ils s’accompagnent de réunions des cortes. La ville offre de nombreuses possibilités d’hébergement ; pour le roi l’Aljafería, restructurée en véritable palais par les rois catholiques, pour les nobles, des palais disséminés dans la ville, dont le type mudéjar se définit alors, pour les plus humbles, les locations chez l’habitant. Ses couvents et sa cathédrale abritent les plus grandes réunions.Pour la venue du roi, la ville organise les entrées les plus somptueuses et manifeste bruyamment sa joie. Mais la venue du roi et de sa cour contraint les autorités à redoubler de vigilance pour éviter les conflits et violences politiques et pour faire face aux perturbations économiques, surtout la hausse des prix.Zaragoza, capital reconocida de Aragón, padece en el siglo XV de la ausencia de sus reyes. Las escasas estancias de sus soberanos representan verdaderos acontecimientos, tanto más cuanto que van acompañadas de reuniones de las Cortes. La ciudad ofrece numerosas posibilidades de alojamiento: para el Rey, la Aljafería, restructurada por los Reyes Católicos en palacio; para los nobles, los palacios diseminados por la ciudad, cuyo estilo mudéjar se define por entonces; para los humildes, alquileres en casas particulares. Sus conventos y su catedral albergan de las reuniones más importantes. Para recibir al Rey, la ciudad organiza las entradas suntuosas y manifiesta ruidosamente su alegría. Pero la llegada del Rey y de su corte obliga a las autoridades a redoblar la vigilancia para evitar los conflictos y las violencias y para hacer frente a las perturbaciones económicas (alza de los precios.

  1. Fonder et acheter, étudier et s’approprier, construire et reconfigurer

    OpenAIRE

    Astafieva, Elena

    2016-01-01

    Cet article est consacré à l’analyse des activités des acteurs russes menées à Jérusalem après la guerre de Crimée. Il présente le cas particulier de la transformation par les Russes d’un espace ‑ « domaine copte » ‑ situé au cœur de Jérusalem, près du Saint-Sépulcre, et il montre ainsi que cette transformation s'opère en trois temps en lien avec trois types d’action : le temps de la fondation des institutions russes « en Orient » et de l’acquisition des terres à Jérusalem (1857-1864) ; le te...

  2. Didaktisk design af et pervasive learning game

    DEFF Research Database (Denmark)

    Oldenskov, Joakim; Westergren, Jens; Frank, Anders Bredahl

    2010-01-01

    Projektet er et studie i det teoretiske grundlag for, og en empirisk undersøgelse, af en prototype for et didaktisk design af et pervasive learning game. Der arbejdes overordnet med udviklingen af et didaktisk design af et læringsspil som kombinerer mobile teknologier, det fysiske rum og eleverne...... game med en analyse af legen og motivationen iboende spillet. I det læringsteoretiske felt inddrages og diskuteres forskellige tilgange til læring og pervasive games, som alle repræsenterer et socialkonstruktivistisk syn på læring. Det didaktiske design danner grundlag for et prototypedesign af et...... spilkoncept, hvor remediering og it-kompetencer inddrages som vigtige teoretiske faktorer. Undersøgelsesdesignet er rettet mod elever i Folkeskolen igennem brugen af fokusgruppeinterviews, og dataindsamlingen er foretaget med video. Eleverne udtrykker sig i undersøgelsen klart omkring konceptets...

  3. Dispositifs semi-conducteurs pour biodetection photonique et imagerie hyperspectrale

    Science.gov (United States)

    Lepage, Dominic

    La creation d'un microsysteme d'analyse biochimique, capable de livrer des diagnostics preliminaires sur la quantification d'elements pathogenes, est un defi multidisciplinaire ayant un impact potentiel important sur la majorite des activites humaines en sante et securite. En effet, un dispositif integre, peu dispendieux et livrant des resultats facilement interpretables, permettrait une vulgarisation des capacites de biodetection a travers differents domaines d'applications societaires et industriels. Le present document se concentre sur l'integration monolithique d'une methode de biocaracterisation dans le but de generer un transducteur miniaturise et efficace, element central d'un microsysteme de detection. Le projet de recherche ici presente vise l'etude de l'applicabilite d'un capteur plasmonique integre par l'entremise de nanostructures semi-conductrices aux proprietes quantiques et luminescentes. L'approche presentee est globale; c'est-a-dire qu'on vise a repondre aux questions fondamentales impliquant la comprehension des phenomenes photoniques, le developpement et la fabrication des dispositifs, les methodes de caracterisations possibles ainsi que l'application d'un transducteur SPR integre a la biodetection. En d'autres termes : dans quelles circonstances et comment un transducteur plasmonique integre doit-il etre realise pour l'application a la detection delocalisee d'elements pathogenes? Dans le but d'engendrer un instrument simple a l'echelle de l'usager, l'integration de la connaissance a l'echelle du design est donc effectuee. Ainsi, des capteurs plasmoniques monolithiques sont concus a l'aide de modeles theoriques ici presentes. Un instrument de mesure hyperspectrale conjuguee permettant de cartographier directement la relation de dispersion des plasmons diffractes a ete construit et teste. Cet instrument est employe a la cartographie d'elements de diffusion. Finalement, une demonstration du fonctionnement du dispositif, appliquee a la

  4. Recherche du boson de Higgs dans l'état final dimuonique et étude de l'asymétrie de production de la paire top antitop avec l'expérience DO auprès du Tevatron; Higgs boson search in the dimuonique final state and study of the top pair antitop production asymmetry with the DO experiment at the Tevatron.

    Energy Technology Data Exchange (ETDEWEB)

    Fauré, Alexandre [University of Paris-Sud, Orsay (France)

    2014-06-03

    Two high energy particle physics analyses are presented in this PhD report using events with two leptons oppositely charged and with missing transverse energy. These events are selected using 9.7 fb-1 of total pp collisions data collected with the DØ detector at the TeVatron at √s=1.96 TeV.The first analysis is the research of the Higgs boson decaying in the H→WW→μνμν channel. No significant excess above the background prediction is observed.Upper limits on Higgs boson production cross-section are computed in the standard model framework but also in the 4th generation of fermions and in the fermiophobic coupling to Higgs boson hypotheses. In order to validate the research methodology, the W boson pair production cross-section is measured.The second analysis is the measurement of the forward-backward asymmetry of the tt pair production. This is the first measurement in the dileptonic channel at DØ experiment. In this context, a new tt pair kinematic reconstruction is used (matrix element method) to give a raw measurement of the forward-backward asymmetry. Thanks to a dedicated calibration method, we give a final measurement of AFB=18.0 ± 6.0 (stat) ± 3.3 (syst).

  5. Extragenital lichen sclerosus et atrophicus

    Directory of Open Access Journals (Sweden)

    Leelavathy Ganesan

    2015-01-01

    Full Text Available Lichen sclerosus et atrophicus (LSA is a chronic inflammatory dermatosis with anogenital and extragenital presentations. Extragenital lichen sclerosus is most common on the neck, shoulders and upper trunk. Linear lesions are uncommon in LSA. We report a case of linear extragenital LSA involving forehead and scalp, along with grouped white papules of LSA in the right side of the back in a postmenopausal woman. The patient showed atypical clinical presentation of LSA in face which clinically mimicked ′en coup de sabre′ as seen in morphea, but other clinical features suggested the diagnosis of LSA and the histopathological findings confirmed it.

  6. Sociologie et sociolinguistique du franbreu

    Directory of Open Access Journals (Sweden)

    Ben-Rafael Eliezer

    2014-07-01

    Full Text Available Des enquêtes ont montré que plus de 20 % des adultes israéliens ont quelque connaissance du français. Parmi cette population, 250 000 à 300 000 personnes pouvaient être considérées comme francophones. Le français a affirmé une présence importante – pas toujours reconnue – dès la phase cruciale du développement de cette société. Une immigration de masse suivit la création de l’État (1948 dont la population a quasiment triplé en moins de deux décennies, avec l’arrivée de plus de 200 000 immigrants d’Afrique du Nord et des dizaines de milliers des Balkans et du Moyen-Orient. Beaucoup parmi ces immigrants étaient francophones – le français étant pour eux soit la première langue soit une deuxième qu’ils contrôlaient. C’est depuis lors que la présence du français est devenue démographiquement significative dans la société israélienne, se prolongeant de nos jours dans l’immigration en provenance de France qui se poursuit jusqu’à aujourd’hui. Comme nous l’avons montré ailleurs (Ben-Rafael et Ben-Rafael 2013, Israël fait l’expérience simultanée de plusieurs francophonies originales qui diffèrent par leurs sources aussi bien que leurs expressions (Ben-Rafael et Ben-Rafael 2013. Un premier modèle est le « français vernaculaire » (voir Boudras-Chapon 2008 amené par les immigrants de classes défavorisées arrivant d’Afrique du nord qui se sont regroupés en Israël. Leur français n’a offert qu’une faible résistance à l’hébreu qui s’est imposée dans le processus d’intégration. Un bilinguisme fonctionnant sur un mode additif au début qui s’est mué progressivement en modèle soustractif. Un second modèle concerne les immigrants de même origine ainsi que des immigrants francophones d’autres pays (pays balkaniques, Turquie ou Egypte formant une strate instruite de classe moyenne, pour qui le français était d’utilisation quotidienne aussi bien que de langue de

  7. Des guides et des cartes

    OpenAIRE

    Mathieu, Patricia; Maryvonne LE BERRE; Jean-Marc ROCHE

    1987-01-01

    Consultés pour leurs références régionales ou locales, les guides touristiques donnent aussi une vision globale de la France qui est rarement perçue par l'utilisateur. Différents types de représentations cartographiques ont été choisis pour montrer, à partir d'informations simples, découpage en «régions» touristiques, étoilement des sites et des espaces touristiques, cette image de la France.

  8. Des guides et des cartes

    Directory of Open Access Journals (Sweden)

    Patricia MATHIEU

    1987-12-01

    Full Text Available Consultés pour leurs références régionales ou locales, les guides touristiques donnent aussi une vision globale de la France qui est rarement perçue par l'utilisateur. Différents types de représentations cartographiques ont été choisis pour montrer, à partir d'informations simples, découpage en «régions» touristiques, étoilement des sites et des espaces touristiques, cette image de la France.

  9. Algorithmique et langages de programmation

    OpenAIRE

    Arsac, Jacques

    1991-01-01

    Dans les débuts de l'informatique, un cours de programmation consistait essentiellement en une description des organigrammes, comme représentation graphique de la structure d'un programme (tellement embrouillée qu'il fallait bien en passer par là), et la liste des instructions d'un langage : les constantes entières s'écrivent avec des chiffres sans point décimal ni partie exposant, un identificateur... l'instruction LIRE, etc. Pour le reste, l'apprenti programmeur devait se débrouiller tout s...

  10. Entrepreneuriat Social et Participation Citoyenne

    OpenAIRE

    Sophie Larivet; Francois Brouard; Ouafa Sakka

    2010-01-01

    L'entrepreneuriat social est un concept émergeant, notamment dans les sciences de l'administration. Pourtant, en dehors d'une pratique directoriale spécifique, il est aussi une forme de participation citoyenne trop méconnue. L'objectif de cet article, basé sur une revue de la littérature et une approche théorique, est de présenter le concept d'entrepreneuriat social afin de mieux saisir son positionnement par rapport à la participation citoyenne. L'entrepreneuriat social constitue une forme p...

  11. CSR i et foranderligt samfund

    OpenAIRE

    Meedom, Maria; Stokholm, Helle; Miara, Denice; á Lakjuni, Heidi; Dayyani, Navid

    2012-01-01

    CSR i et foranderligt samfund Dette projekt har fokus på samfundsansvar. I projektet henviser vi løbende til elementer fra Anthony Giddens strukturationsteori og modernitetsanalyse. Vi undersøger hvad der ligger i begrebet CSR og hvordan udviklingen af CSR har været i Danmark. Vi diskuterer de samfundsmæssige konsekvenser af forskellige holdninger til CSR. Dette gør vi ud fra teori af Friedman og Henderson, der ikke mener det er virksomhedernes ansvar og Freeman og Porter & Kramer der arg...

  12. Casanova et D’Alembert

    OpenAIRE

    Aleksić, Branko

    2010-01-01

    De son premier livre Confutazione della Storia del Governo Veneto d’Amelot de la Houssaie (Lugano, 1768) jusqu’à son dernier publié en français (Dresde, 1797), dans sept ou huit textes de genres différents – notes, critique de philosophie, correspondance, enfin mémoires - Giacomo Casanova (1725-1798) se réfère à la pensée de D’Alembert. Ces textes de Casanova portent sur différents thèmes humanistes : la philosophie et la critique de l’éducation (Della filosofia e de filosofi, 1774-1783 comme...

  13. Le changement comme tradition dans la recherche et la formation a la recherche en biotechnologie et en peripherie Etude de cas en sciences de la sante, sciences naturelles et genie

    Science.gov (United States)

    Bourque, Claude Julie

    luttes universitaires que scientifiques, concentrees sur la negociation du capital scientifique, symbolique et economique en jeu dans la formation doctorale, dans les carrieres auxquelles elle mene, et dans les qualites du titre de Ph.D. Au final, la confusion entre des logiques opposees peut etre reduite en reinterpretant le changement comme tradition du champ scientifique. Mots-cles Sociologie, education, enseignement superieur, science et technologie, biotechnologie, formation doctorale, champ scientifique, reseaux sociaux

  14. Estimating Riparian ET through Remote Sensing

    Science.gov (United States)

    Samani, Z.; Bawazir, S.; Bleiweiss, M.; Skaggs, R.; Schmugge, T.

    2005-12-01

    Riparian evapotranspiration (ET) along the Rio Grande River has become a major hydrological as well as political issue in New Mexico. The State of New Mexico has spent millions of dollars in recent years to eradicate riparian vegetation without being able to quantify the change in regional ET. Many studies have focused on measuring evapotranspiration of individual riparian vegetation types, mainly saltcedar and native cottonwood. However, the riparian vegetation on the Middle Rio Grande varies in density and species.. Spatial variation in climate, soil type and depth to groundwater causes variation in ET, as well. It is obvious that in order to obtain more accuracy in measurements, multiple sampling points are needed; thus, making the process costly and impractical An alternative solution, which is also cost-effective, is measuring ET by using remote sensing technology. Remote sensing combines regional satellite data with localized ET measurement to calculate regional ET. REEM (Regional ET Estimation Model) is a process that uses the energy balance at the top of the canopy to estimate ET. REEM has been using ASTER images for values of surface temperature, albedo and NDVI to calculate net radiation (Rn), ground heat flux (G) and sensible heat flux (H). The ET is then calculated as residual of the energy components. The REEM model is being used to calculate regional ET values for the Riparian vegetation of the Middle Rio Grande. The paper compares the ET values for various vegetation types using remote sensing and ET derived from Eddy Covariance Flux Towers.

  15. 76 FR 71355 - United States et al. v. Blue Cross and Blue Shield of Montana, Inc. et al.; Proposed Final...

    Science.gov (United States)

    2011-11-17

    ... affordable prices can attract businesses and jobs to a state or region, and higher health-insurance prices.... *Attorney of Record. FOR PLAINTIFF STATE OF MONTANA: Steve Bullock, Attorney General of Montana. James...

  16. 75 FR 5120 - United States, et al. v. Stericycle, Inc., et al.; Proposed Final Judgment and Competitive Impact...

    Science.gov (United States)

    2010-02-01

    ... hospitals, large laboratories, and other large medical facilities that generate large amounts of infectious..., materials, supplies, on- or off-site warehouses or storage facilities; all licenses, permits, and..., ledgers, accounts, records, data, and documents in the possession, custody, or control of...

  17. 76 FR 5439 - United States, et al. v. Comcast Corp., et al.; Proposed Final Judgment and Competitive Impact...

    Science.gov (United States)

    2011-01-31

    ... streaming models (e.g., Hulu Plus, Netflix), per-program rentals (e.g., Apple iTunes, Vudu), and hybrid... Apple iTunes, Amazon, and Netflix. 53. Comcast and other MVPDs have significant concerns over emerging...., Netflix and Amazon), and deploying its ``authenticated'' online, on-demand service. Consumers...

  18. 76 FR 34750 - United States et al. v. Comcast Corp., et al.; Public Comments and Response on Proposed Final...

    Science.gov (United States)

    2011-06-14

    ... Antitrust Institute in U.S. vs. Comcast Corp., General Electric Co., and NBC Universal, Inc., pursuant to... distribution and diversity in the media. In June 2010, the AAI filed comments with the Federal Communications..., and enjoy fewer benefits from innovation.\\7\\ \\7\\ Supra note 5, at para. 2. Herein lies the...

  19. 77 FR 51048 - United States et al. v. Verizon Communications Inc. et al.; Proposed Final Judgment and...

    Science.gov (United States)

    2012-08-23

    ... area in exchange for a commitment to build out broad cable coverage. The copper-wire telephone network... Communications, Inc., and Bright House Networks, LLC, would violate Section 1 of the Sherman Act, 15 U.S.C. 1... 20530 (telephone: 202-514-2481), on the Department of Justice's Web site at...

  20. 78 FR 32443 - United States, et al. v. Cinemark Holdings, Inc., et al.; Proposed Final Judgment and Competitive...

    Science.gov (United States)

    2013-05-30

    ... Cinemark Holdings, Inc. of movie theatres and related assets from Rave Cinemas, LLC would violate Section 7... owned and operated by Rave Holdings, LLC (``Rave Cinemas''). Cinemark is a significant competitor to Rave Cinemas in the exhibition of first-run, commercial movies in the area in and around Voorhees...

  1. Le voyageur et le touriste

    Directory of Open Access Journals (Sweden)

    André Rauch

    2011-06-01

    Full Text Available Les guides qui paraissent dans la première moitié du XIXe siècle, en langue allemande, anglaise et française, ont introduit des usages. Ils ont défini une manière de voyager, arrêté les itinéraires et indiqué les sites ou les lieux qu’il convenait de visiter. Lorsque paraissent les guides pour automobilistes au début du XXe siècle, la route permet d’autres formes de vagabondage, dont la gastronomie est un exemple illustratif. Dans les années soixante-dix, les nouveaux guides ont suscité d’autres curiosités, ils ont aussi indiqué au touriste les budgets qui leur permettraient de s’orienter dans la consommation touristique.Guides published during the first half of 19th century, in German, English and French languages have introduced some customs. They have defined a way of travelling, made up the routes and indicated the places and settings one has to visit. When guides for motorists are published at the beginning of the 20th century, the road allows other forms of wandering, among which gastronomy is an illustrative example. During the seventies, the new guides aroused other curiosities, they also indicated to the tourists the budgets that would allow them to find their way inside tourist consuming.

  2. Desordre et phenomenes de localisation

    CERN Document Server

    Lacoin, Hubert

    2009-01-01

    Cette these est consacree a l' etude de differents modeles aleatoires de polymeres. On s'interesse en particulier a l'influence du desordre sur la localisation des trajectoires pour les modeles d'accrochage et pour les polymeres diriges en milieu aleatoire. En plus des modeles classiques dans Zd, nous abordons l' etude de modeles dit hierarchiques, construits sur une suite de reseaux auto-similaires, tres present dans la litterature physique. Les resultats que nous avons obtenus concernent principalement l' energie libre et le phenomene de surdiffusivite. En particulier, nous prouvons: (1) la pertinence du desordre a toute temperature dans pour le modele d' accrochage desordonne en dimension 1 + 1, (2) l' occurence d' un desordre tres fort a toute temperature en dimension 1 + 2 pour les polymeres diriges en milieu aleatoire. This thesis studies models of random directed polymers. We focus on the influence of disorder on localization of the trajectories for pinning model and directed polymers in random environ...

  3. Stylistique et statistiques Le corpus textuel et Hyperbase

    OpenAIRE

    Magri-Mourgues, Véronique

    2010-01-01

    L'analyse de données textuelles est désormais admise comme partenaire de la linguistique textuelle. L'intrusion des modèles mathématiques dans l'explication des textes littéraires peut se manifester par exemple dans des journées qui lui sont entièrement consacrées, où se côtoient linguistes, stylisticiens et informaticiens, les Journées d'Analyse statistique des Données Textuelles, qui se tiennent une fois tous les deux ans. L'avantage reconnu du traitement informatisé est double : il porte s...

  4. Elements optiques diffractifs concus avec des ouvertures trapezoidales et polygonales et de nouveaux algorithmes d'optimisation

    Science.gov (United States)

    Gillet, Jean-Numa

    Nous avons realise de nouveaux encodages et algorithmes d'optimisation destines aux elements optiques diffractis et hologrammes generes par ordinateur (HGO). Un algorithme novateur de condensation rapide simulee iterative permet la resolution de problemes avec plusieurs milliers de variables. Malgre un horaire de refroidissement rapide, de nombreuses solutions sous-optimales sont evitees par des reechelonnements de temperature ramenant le systeme a l'equilibre thermodynamique. L'etat final atteint par le nouvel algorithme a une energie beaucoup plus basse que celle obtenue par d'autres algorithmes. Les generateurs de tableaux irreguliers de points lumineux concus avec la condensation rapide simulee iterative et des ouvertures trapezoidales de hauteurs variables presentent de meilleures performances et beaucoup moins d'ouvertures comparativement a ceux concus avec d'autres methodes. Nous avons aussi realise un HGO multiplexe de taille extremement grande forme d'ouvertures polygonales et le nouvel algorithme iteratif de conception des sous-hologrammes pour generer des images de tres grandes dimensions. Nous utilisons la transformee d'Abbe pour calculer la diffraction des ouvertures polygonales, ce qui permet d'accelerer substantiellement le calcul de la transformee de Fourier de l'hologramme total. La taille en pixels de l'HGO multiplexe forme d'ouvertures polygonales est plus de mille fois superieure a celle d'un HGO conventionnel et sa fenetre objet beaucoup plus grande. L'HGO multiplexe forme d'ouvertures polygonales presente des performances beaucoup plus elevees que celles d'HGO conventionnels ou multiplexes concus avec les methodes precedentes.

  5. Concentration horizontale et relations verticales

    OpenAIRE

    Marie-Laure Allain; Saïd Souam

    2005-01-01

    This paper studies horizontal mergers in vertically related markets. In atwo-level Cournot model, with an intermediate and a final market, we show thatdownstream mergers inducing size effects are, ceteris paribus, more profitablethan upstream ones. Moreover, a merger at one level reduces the incentivesto merge at the other level. Endogenizing the firms’ decisions to merge byconsidering a merger game supports the previous results.

  6. Chapitre II. Administration et gestion domaniales

    OpenAIRE

    Aristide, Isabelle

    2014-01-01

    L’administration et la gestion des domaines de Sully est comparable à celles de l’ensemble de sa fortune : il faut, là encore, souligner son intervention personnelle, et plus encore peut-être celui de la duchesse. Sully affirma et remplit pleinement son rôle de seigneur féodal et, tout en employant des agents et des comptables qui traitaient ordinairement de ses affaires générales, il fit également appel aux officiers locaux, en place lorsqu’il acheta ses terres ou bien installé par lui. A. L...

  7. Induction of host defences by Rhizobium during ineffective nodulation of pea (Pisum sativum L.) carrying symbiotically defective mutations sym40 (PsEFD), sym33 (PsIPD3/PsCYCLOPS) and sym42.

    Science.gov (United States)

    Ivanova, Kira A; Tsyganova, Anna V; Brewin, Nicholas J; Tikhonovich, Igor A; Tsyganov, Viktor E

    2015-11-01

    Rhizobia are able to establish a beneficial interaction with legumes by forming a new organ, called the symbiotic root nodule, which is a unique ecological niche for rhizobial nitrogen fixation. Rhizobial infection has many similarities with pathogenic infection and induction of defence responses accompanies both interactions, but defence responses are induced to a lesser extent during rhizobial infection. However, strong defence responses may result from incompatible interactions between legumes and rhizobia due to a mutation in either macro- or microsymbiont. The aim of this research was to analyse different plant defence reactions in response to Rhizobium infection for several pea (Pisum sativum) mutants that result in ineffective symbiosis. Pea mutants were examined by histochemical and immunocytochemical analyses, light, fluorescence and transmission electron microscopy and quantitative real-time PCR gene expression analysis. It was observed that mutations in pea symbiotic genes sym33 (PsIPD3/PsCYCLOPS encoding a transcriptional factor) and sym40 (PsEFD encoding a putative negative regulator of the cytokinin response) led to suberin depositions in ineffective nodules, and in the sym42 there were callose depositions in infection thread (IT) and host cell walls. The increase in deposition of unesterified pectin in IT walls was observed for mutants in the sym33 and sym42; for mutant in the sym42, unesterified pectin was also found around degrading bacteroids. In mutants in the genes sym33 and sym40, an increase in the expression level of a gene encoding peroxidase was observed. In the genes sym40 and sym42, an increase in the expression levels of genes encoding a marker of hypersensitive reaction and PR10 protein was demonstrated. Thus, a range of plant defence responses like suberisation, callose and unesterified pectin deposition as well as activation of defence genes can be triggered by different pea single mutations that cause perception of an otherwise

  8. Concentration, financiarisation et relations entre les industries de la culture et industries de la communication

    Directory of Open Access Journals (Sweden)

    Philippe Bouquillion

    2012-09-01

    Full Text Available Cette contribution se propose d’étudier comment les transformations récentes dans les modalités de concentration, de financiarisation et d’articulation entre industries de la culture et industries de la communication contribuent à transformer les logiques qui président à la constitution des groupes industriels dans ces deux domaines et comment elles impactent les rapports de force entre ces deux types d’industries. Trois transformations principales sont relevées. Tout d’abord, l’entrée des acteurs des industries de la communication dans l’économie des contenus est massive bien que surtout concentrée sur l’aval des filières. Elle contribue aux difficultés des acteurs des industries de la culture. Ensuite, l’insertion dans la financiarisation dynamise surtout, dans la période actuelle, les acteurs des industries de la communication. Enfin, les groupes des industries de la culture ont connu, pour nombre d’entre eux, une réduction de leur périmètre et sont menacés d’être dominés par les grands acteurs des industries de la communication, lesquels ont conquis une puissance très forte depuis le milieu des années 2000.This contribution analyses how the recent transformations in the modalities of concentration, financialization and articulation between cultural industries and communications industries contribute to transform the logics which preside over the constitution of the industrial groups in these two domains and how they impact on the balance of power between these two types of industries. Three main transformations are found. First of all, the entrance of the actors of the communications industries to the economy of the contents is massive although especially concentrated downstream of the sectors. It contributes to the difficulties of the actors of the cultural industries. Then, the insertion in the financialization revitalizes especially, for the current period, the actors of the communications

  9. Le cheval et ses patrimoines

    OpenAIRE

    Galey, Bertrand-Pierre

    2012-01-01

    Ce numéro de la revue In Situ est consacré à un patrimoine culturel tout à la fois omniprésent et méconnu, constitué des très nombreux témoignages de l’histoire du cheval en France, qu’il s’agisse de vestiges archéologiques, de documents d’archives, de livres anciens, d’objets scientifiques, de bâtiments d’écuries, de manèges, d’hippodromes, de harnachements, de voitures hippomobiles, mais aussi de traditions, de pratiques, de savoir-faire, etc. Il propose au lecteur une approche transdiscipl...

  10. Le livre et ses espaces

    OpenAIRE

    Benhamouda, Kheira; Bessard-Banquy, Olivier; Brown, Llewellyn; Campaignolle-Catel, Hélène; Carreto, Carlos F. Clamote; Chol, Isabelle; Cooke, Dervila; Delambre, Raymond; Gâta, Anca; Guillot, Catherine; Hennebert, Jérôme; Kouakou, Jean-Marie; Koudriavsteva, Olessia; Lelièvre, Valérie; Milon, Alain

    2012-01-01

    LA CONNAISSANCE DU LIVRE se rattache ici à la mise en jeu dialectique de termes apparemment éloignés : « livre » et « espace ». Le livre ne se réduit donc pas à son support matériel. Notre intention n’est pas non plus de délimiter tous les espaces possibles du livre qu’ils soient physique, esthétique, littéraire, métaphysique ou anthropologique. Il s’agit plutôt de proposer au lecteur un voyage à travers l’espace dans le livre - la page, le texte…, l’espace pour le livre - la bibliothèque, la...

  11. Le smartphone et le concert

    OpenAIRE

    Heuguet, Guillaume

    2014-01-01

    International audience Face à la baisse des ventes de musique enregistrée, les maisons de disques mettent de plus en plus le concert au centre de leur économie : les signatures de Because Music bénéficient aussitôt du travail de tourneur de sa filiale Corrida ; Live Nation, mastodonte de l'organisation de tournées, s'est rendu propriétaire des futurs enregistrements de Jay-Z. Une multitude d'acteurs est aujourd'hui pris dans un mélange de compétition et de coopération dans l'écosystème mus...

  12. Langage C++ et calcul scientifique

    OpenAIRE

    Saramito, Pierre

    2005-01-01

    La simulation numérique est devenue essentielle dans de nombreux domaines tels que la mécanique des fluides et des solides, la météo, l'évolution du climat, la biologie ou les semi-conducteurs. Elle permet de comprendre, de prévoir, d'accéder là où les instruments de mesures s'arrêtent. Ce livre présente des méthodes performantes du calcul scientifique : matrices creuses, résolution efficace des grands systèmes linéaires, ainsi que de nombreuses applications à la résolution par éléments fini...

  13. La sociologie et le monde

    OpenAIRE

    Atlani-Duault, Laëtitia (ed.); Dufoix, Stéphane; Wieviorka, Michel

    2014-01-01

    Figure 1 Immanuel Wallerstein Socio : Nous traversons depuis quelques années une période où le mot « crise » est sur toutes les lèvres : crise de l’euro et de l’Europe, crise financière… On sait que le thème de la crise des sciences sociales est récurrent depuis au moins les années 1950. Estimez-vous que les sciences sociales sont, elles aussi, actuellement en crise ou qu’elles connaissent plutôt une période d’épanouissement ? Immanuel Wallerstein : La crise générale du système-monde dans le...

  14. Et originalt og komplekst sprog

    Directory of Open Access Journals (Sweden)

    Romá Gubern

    1999-09-01

    Full Text Available Tegneserier er en semiotisk udfordring, fordi de fortæller historier i en kombination af billeder og tekst. Forståelsen af tegneserier involverer derfor såvel narrative som visuelle og lingvistiske koder i kombinationer, der markerer tegneseriens æstetiske egenart. Der er dog kun i begræn- set omfang udviklet en terminologi, der kan anvendes til definitionen af tegneseriens særlige tegn og tegnkombinationer. I denne artikel giver Romá Gubern en definition på tegneserien, hvorefter han med et semio- tisk udgangspunkt gennemgår de særtræk, der karakteriserer tegne- serien, og foreslår en tegneseriens terminologi.

  15. Sprogtilegnelse i et inkluderende perspektiv

    DEFF Research Database (Denmark)

    Bylander, Helle Iben; Krogh, Trine Kjær

    Denne bog udgør et praksisnært, forskningsbaseret og interaktivt studiemateriale til praktikere og studerende. Den giver mulighed for, at læseren kan gøre sig praktiske erfaringer med sprogarbejde i en tryg ramme. I samarbejde med pædagoger er der til bogen produceret en række film, som – sammen ...... med relevante klip fra YouTube og internetbaserede artikler – ligger på den tilhørende hjemmeside. Læseren afprøver således sin viden via bogens mange øvelser og refleksionsspørgsmål, der tager udgangspunkt i hjemmesidens materiale....

  16. La Balsamine et les Hommes

    OpenAIRE

    Monty, Arnaud; Cristofoli, Sara

    2007-01-01

    Les invasions biologiques sont actuellement considérées comme l’une des atteintes majeures à la biodiversité. Néanmoins, elles sont encore souvent mal perçues du grand public. Ce court-métrage s’inscrit dans ce contexte et présente le cas de la Balsamine géante (Impatiens glandulifera ROYLE), espèce très esthétique, mais aussi particulièrement dommageable pour la flore des berges de cours d’eau, dans son aire d’invasion. La Balsamine fut introduite en Europe il y a plus d’un siècle, depuis le...

  17. Les pauvres et la consommation

    OpenAIRE

    Lazarus, Jeanne

    2006-01-01

    L’histoire des pauvres dans la société de consommation depuis la seconde guerre mondiale fut d’abord celle de l’enrichissement et de l’accès au confort des classes populaires pendant les Trente Glorieuses. La misère qui subsistait dans les bidonvilles intéressait peu les chantres du progrès, qui qualifiaient d’inadaptés ceux qui restaient à l’écart. D’autant que les organisations ouvrières comme les sciences sociales s’intéressaient peu à la très grande pauvreté, occupées qu’elles étaient à s...

  18. Approvisionnement et gestion des silex

    Directory of Open Access Journals (Sweden)

    Christophe Delage

    2008-06-01

    Full Text Available Les études de provenance des vestiges présents sur les sites préhistoriques se sont particulièrement développées ces dernières années au Proche-Orient. Des matériaux tels que l’ocre, le basalte ou l’obsidienne, ont ainsi fait l’objet de caractérisations archéométriques détaillées. En ce qui concerne le silex, on savait qu’il était présent en abondance au nord d’Israël. Quelques rares études ponctuelles ont montré le potentiel de recherches sur l’origine et les méthodes de taille des silex pré...

  19. Stanley Kubrick et la musique

    OpenAIRE

    Giuliani, Elizabeth

    2010-01-01

    Diffusion d’un extrait du Docteur Folamour (générique de début)    “I think music is one of the most effective ways of preparing an audience and reinforcing points that you wish to impose on it. The correct use of music, and this includes the non-use of music, is one of the greatest weapons that the film maker has at his disposal.” Très formaliste, Stanley Kubrick a bien placé la musique au cœur de sa construction cinématographique et lui a conféré une véritable fonction rhétorique. Il e...

  20. Le vieillard et le chevalier

    Directory of Open Access Journals (Sweden)

    Mélanie JECKER

    2008-04-01

    Full Text Available Éléments introductifs Ces réflexions initiales n’auront pas pour point de départ le rappel du problème posé par la présence de vieillards toujours plus nombreux au sein de nos sociétés contemporaines. Je ne nie pas l’importance ni l’intérêt du phénomène relativement récent du vieillissement de la population en Occident, mais ce constat ne fut pas à l’origine de mon travail. Ce qui a porté ma curiosité vers le thème des âges de la vie n’est pas mon propre présent mais l’attrait et même, oserai...

  1. Echantillonnage, interpolation et détection. Applications en imagerie satellitaire.

    OpenAIRE

    Almansa, Andrés

    2002-01-01

    Cette thèse aborde quelques-uns des problèmes qui surviennent dans la conception d'un système complet de vision par ordinateur : de l'échantillonnage à la détection de structures et leur interprétation. La motivation principale pour traiter ces problèmes a été fournie par le CNES et la conception des satellites d'observation terrestre, ainsi que par les applications de photogrammétrie et vidéo-surveillance chez Cognitech, Inc. pendant les étapes finales de ce travail, mais les techniques déve...

  2. ATLAS jet and missing ET reconstruction, calibration, and performance

    CERN Document Server

    Berta, P; The ATLAS collaboration

    2014-01-01

    ATLAS has achieved a very high precision on jet and missing transverse energy performance by the use of advanced calorimeter-based topological clustering and local cluster calibration, event-by-event pile-up subtraction methods, and in situ techniques to correct for the residual jet energy response difference between data and simulation. Tracking information is being combined with calorimeter to further improve the jet and missing ET performance. ATLAS has also commissioned several new powerful tools for for the analysis and interpretation of hadronic final states at the LHC such as jet substructure, jet mass, quark-gluon discrimination, and jet tagging tools for the identification of boosted heavy particles. An overview of the reconstruction, calibration and performance of jets, missing ET, and jet substructure and tagging at ATLAS is presented.

  3. Reply to Pachai et al.

    Science.gov (United States)

    Harrison, William J; Bex, Peter J

    2016-05-01

    Peripheral vision is fundamentally limited by the spacing between objects. When asked to report a target's identity, observers make erroneous reports that sometimes match the identity of a nearby distractor and sometimes match a combination of target and distractor features. The classification of these errors has previously been used to support competing 'substitution' [1] or 'averaging' [2] models of the phenomenon known as 'visual crowding'. We recently proposed a single model in which both classes of error occur because observers make their reports by sampling from a biologically-plausible population of weighted responses within a region of space around the target [3]. It is critical to note that there is no probabilistic substitution or averaging process in our model; instead, we argue that neither substitution nor averaging occur, but that these are misclassifications of the distribution of reports that emerge when a population response distribution is sampled. This is a fundamentally different way of thinking about crowding, and on this basis we claim to have provided a mechanism unifying categorically distinct perceptual errors. Our goal was not to model all crowding phenomena, such as the release from crowding when target and flanks differ in color or depth [4]. Pachai et al.[5] have suggested that our model is not unifying because it inaccurately predicts perceptual performance for a particular stimulus. Although we agree that our model does not predict their data, this specific demonstration overlooks the critical aspect of the model: perceptual reports are drawn from a weighted population code. We show that Pachai et al.'s [5] own data actually provide evidence for the population code we have described [3], and we suggest a biologically-plausible analysis of their stimuli that provides a computational basis for their 'grouping' account of crowding.

  4. Reply to Pachai et al.

    Science.gov (United States)

    Harrison, William J; Bex, Peter J

    2016-05-01

    Peripheral vision is fundamentally limited by the spacing between objects. When asked to report a target's identity, observers make erroneous reports that sometimes match the identity of a nearby distractor and sometimes match a combination of target and distractor features. The classification of these errors has previously been used to support competing 'substitution' [1] or 'averaging' [2] models of the phenomenon known as 'visual crowding'. We recently proposed a single model in which both classes of error occur because observers make their reports by sampling from a biologically-plausible population of weighted responses within a region of space around the target [3]. It is critical to note that there is no probabilistic substitution or averaging process in our model; instead, we argue that neither substitution nor averaging occur, but that these are misclassifications of the distribution of reports that emerge when a population response distribution is sampled. This is a fundamentally different way of thinking about crowding, and on this basis we claim to have provided a mechanism unifying categorically distinct perceptual errors. Our goal was not to model all crowding phenomena, such as the release from crowding when target and flanks differ in color or depth [4]. Pachai et al.[5] have suggested that our model is not unifying because it inaccurately predicts perceptual performance for a particular stimulus. Although we agree that our model does not predict their data, this specific demonstration overlooks the critical aspect of the model: perceptual reports are drawn from a weighted population code. We show that Pachai et al.'s [5] own data actually provide evidence for the population code we have described [3], and we suggest a biologically-plausible analysis of their stimuli that provides a computational basis for their 'grouping' account of crowding. PMID:27166690

  5. Parodie et publicité

    OpenAIRE

    Bonhomme, Marc

    2010-01-01

    This article has a double aim. On the one side, it proposes a critical re-examination of parody in the frame of represented discourse and interdiscursivity practices. On the other side, it wants to study the function of parody in advertising communication. The following points will be emphasized: the importance of comparison between parody and pastiche in advertising, the procedures of advertising parody, the role of parody in ads transmission. Finally, the parody appears as a media phenomeno...

  6. CO{sub 2} reduction: potential of heat pumps when used together with combined heat and power units; Potentiel energetique des pompes a chaleur combinees au couplage chaleur-force. Pour une reduction maximale des emissions de CO{sub 2} et pour une production de courant fossile avec reduction des emissions de CO{sub 2} en Suisse - Rapport final

    Energy Technology Data Exchange (ETDEWEB)

    Rognon, F.

    2005-07-01

    This final report for the Swiss Federal Office of Energy (SFOE) presents the results of a project that examined the potential of using heat pumps in combination with Combined Heat and Power (CHP) units as a replacement for fossil-fuel fired heating units in Switzerland. The electrical power produced by the CHP units can be used to provide power for the drives of heat pump systems. The author states that the fossil fuel consumption and the resulting emissions of carbon dioxide can be halved using this combination. Also, even more efficient systems using power produced in larger combined-cycle power generation are discussed. Further examples of how fossil-fired power generation in combination with heat pumps can not only replace fossil-fuelled heating systems but also provide additional electricity too are given. This overview includes figures on the potential of such combined systems.

  7. Voltaire et Montesquieu : attitudes philosophiques et genres textuels

    Directory of Open Access Journals (Sweden)

    Alexandra Kratschmer

    2004-05-01

    Full Text Available Ausgehend von einer komparativen textlinguistischen Analyse der Philosophie de l’histoire von Voltaire und den Considérations sur les causes de la grandeur des Romains et de leur décadence Montesquieus wird gezeigt, in welchem Maße der philosophische Hintergrund und in der Folge das schriftstellerische Selbstverständnis eines Autors auf dessen Textgestaltung Einfluß nimmt. Obwohl beide der genannten Autoren der Tradition der Aufklärung zugeordnet werden, erweisen sie sich als sehr verschieden, was ihre Weltsicht und damit ihre Vertextlichungen betrifft. Während Voltaire an die Möglichkeit einer kontinuierlichen Verbesserung der Welt durch den zivilisatorischen Fortschritt glaubt, sich selbst als engagierten Reformator und seine Texte als Propagandawerkzeuge im Rahmen dieser Mission sieht, diese Texte dementsprechend überzeugend gestalten will und sich daher einer stark manipulativen Rhetorik bedient, sieht Montesquieu die menschliche Geschichte als eine unvermeidliche zyklische Abfolge von Expansion und Verfall und versteht sich daher eher als ein kluger Verwalter des Bestehenden, der mit Hilfe seiner Texte über gewisse Zusammenhänge, die er entdeckt zu haben meint, informieren möchte, der seine Texte stark explikativ gestaltet, um das Verständnis der Leser zu fördern, der aber ohne intensive rhetorisch-manipulative Techniken auskommt.

  8. Foucault et l’Utilitarisme

    Directory of Open Access Journals (Sweden)

    Jean-Pierre Cléro

    2011-05-01

    Full Text Available Foucault a travaillé sur quelques textes de Bentham ; mais il ne s'est, semble-t-il, jamais prononcé sur sa propre affinité avec l’utilitarisme ou sur son rejet de l'utilitarisme, qu'il soit classique ou moderne. Quelques traits permettent de rapprocher la recherche de Foucault de l'utilitarisme : la critique du sujet et de son autonomie est au cœur de ces doctrines. Le vrai peut être pensé comme une construction de part et d'autre ; on pourrait croire que la prise en compte du plaisir est aussi un point commun. Or, il n'en est rien. Il existe chez Foucault un souci du vrai, qui est complètement absorbé par l'utile chez les utilitaristes. Quant au plaisir, il est, chez les utilitaristes, aspiré dans le sillage du bonheur -ce qui n'est pas le cas chez Foucault. Même si ce n'est pas au point où Lacan a pu en tirer parti chez Bentham, la théorie des fictions a été, en se fondant sur d'autres auteurs que Bentham, un fil rouge de la pensée de Foucault.It is well known that Foucault worked on some of Bentham’s texts, but he never seems to have questioned his own affinity with, or rejection of, utilitarianism, be it classical or modern. Foucault’s work however shared some features with utilitarianism: especially the critique of the subject and his or her autonomy. Utilitarian thinkers and Foucault understand truth as a construct and have pleasure as a focal point. But no such encounter ever happened. Foucault cared for the truth, whereas for Utilitarians truth was entirely absorbed within utility. For Utilitarians pleasure followed in the wake of happiness – which was not the case in Foucault. The theory of fictions, however, provides a better meeting ground, though Foucault quoted other thinkers than Bentham.

  9. L'argent et le bonheur

    Institute of Scientific and Technical Information of China (English)

    郑砚心

    2011-01-01

    L'argent et le bonheur,l'un est concret et l'autre ést abstrait.Mais tous les deux sont parmi les sujets les plus traités dans la littérature et dans la vie quotidienne.Maintenant,de plus en plus de personnes aiment mettre les deux ensemble.Il semble que l'argent est devenu le synonyme du bonheur.Mais qu'en est-il en réalité? Chacun a sa propre opinion.Pour dévoiler la relation entre l'argent et le bonheur,il nous faut d'abord connaitre la signification de l'argent et du bonheur.L'argent se produit à mesure de l'évolution de la société.Et le bonheur est un sentiment non-figuratif qui existe depuis l'apparition de l'homme.

  10. L’excellence : impossible sans un personnel stable et expérimenté

    CERN Multimedia

    Association du personnel

    2013-01-01

    Le monde entier avait les yeux fixés sur le CERN ce 4 juillet 2012, quand ATLAS et CMS ont annoncé leur découverte d’une particule « compatible avec le Higgs ». Début 2013 il n’a fallu que quelques jours pour régler le LHC et faire tourner des protons et des ions lourds. Toutes ces découvertes et prouesses techniques n’ont été possibles que grâce à l’expérience et la poursuite de l’excellence d’équipes soudées de techniciens et ingénieurs qui ont conçu, construit, mis en marche, et finalement font fonctionner ce complexe d’accélérateurs unique au monde. Un service d’excellence avec un encadrement adéquat Entre 2002 et 2012 (voir Fig. 1), le nombre d’utilisateurs est passé de 5912 à...

  11. Theories and calculation methods for regional objective ET(evapotranspiration):Applications

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The regional objective ET(evapotranspiration) is defined as the quantity of water that could be con-sumed in a particular region.It varies with the water conditions and economic development stages in the region.It is also constrained by the requirement of benign environment cycle.At the same time,it must meet the demands of sustainable economic growth and the construction of harmony society.Objective ET based water resources distribution will replace the conventional method,which empha-sizes the balance between the water demand and the water supply.It puts focus on the reasonable water consumption instead of the forecasted water demand,which is usually greater than the actual one.In this paper,we calculated the objective ET of 2010 year level in Tianjin by an analysis-integra-tion-assessment method.Objective ET can be classified into two parts:controllable ET and uncontrol-lable ET.Controllable ET includes the ET from irrigation land and the ET from resident land,among which the former can be calculated with soil moisture model and evapotranspiration model,while the latter can be calculated by water use ration and water consumption rate.The uncontrollable ET can be calculated with the distributed hydrological model and the remote sensing monitoring model.The two models can be mutually calibrated.In this paper,eight schemes are put forward based on different portfolios of water resources.The objective ET of each scheme was calculated and the results were assessed and analyzed.Finally,an optimal scheme was recommended.

  12. Vesihuoltolaitosten vesimittareiden etäluenta

    OpenAIRE

    Korhonen, Joonas

    2015-01-01

    Tämä opinnäytetyö tehtiin Helsingin Seudun Ympäristöpalvelut -kuntayhtymän (HSY) vesihuollon toimialalle ja sen tarkoituksena oli selvittää millaisia hyötyjä HSY:n olisi mahdollista saavuttaa asiakkaidensa vedenkulutusta mittaavien vesimittareiden etäluennalla ja minkälaisia haasteita etäluentaan siirtymisellä on. Työn sisältö palvelee vesimittareiden etäluentaa harkitsevia vesilaitoksia. Tässä työssä on erityisesti kuvattu työn tilaajan HSY:n vesimittaroinnin nykytilaa ja etäluennan suht...

  13. Hiérarchie et patronage

    OpenAIRE

    Joukovskaïa-Lecerf, Anna

    2007-01-01

    L’importance des relations de patronage et de clientèle dans les rapports hiérarchiques de travail entre les agents administratifs est bien connue. Au croisement de l’histoire des institutions, des pratiques et des personnels administratifs, le présent article élucide la spécificité du patronage et du clientélisme institutionnels et explore leur rôle dans les rapports de travail. Dans la première section de l’article est illustré le type des relations de travail caractéristiques des secrétari...

  14. La communication entre parents migrants et enseignants

    OpenAIRE

    Frei, Nathalie; Schertenleib, Georges-Alain

    2014-01-01

    La communication entre l’école et la famille semble être une composante essentielle (parmi tant d’autres d’ailleurs !) qui permettrait à l’enfant d’apprendre et d’évoluer sereinement. Elle n’est pourtant pas toujours bien vécue de part et d’autre car il arrive que certains malentendus surviennent. En effet, les enseignants de nos jours ont des contacts avec des familles d’origines très variées et doivent savoir faire preuve d’adaptation face à cette diversité. Même si la tâche est quelques fo...

  15. Enseignants et psychologues: mêmes attentes ?

    OpenAIRE

    Gygax, Florie; Maillefer, Nicole; Baumberger, Bernard

    2011-01-01

    Notre travail de mémoire professionnel nous a été inspiré par le travail de licence d’Andenmatten et Pasca (2006). A la fin de leur travail, ces deux étudiantes affirment avoir constaté des liens complexes entre les enseignants et les psychologues. Une de leurs questions à ce sujet restant en suspens à la fin de leur recherche, aurait pu être posée aux enseignants et aux psychologues scolaires. La question qu’elles ont regretté ne pas avoir posée, et qui nous a particulièrement interpellé...

  16. Internet et commerce électronique

    OpenAIRE

    Benghozi, Pierre-Jean; Licoppe, Christian; Rallet, Alain

    2001-01-01

    Le commerce électronique pose des problèmes économiques et de gestion à la fois bien connus sous certains aspects (dynamiques d'intégration, stratégies de marque) et nouveaux par d'autres (dynamique de constitution des modèles d'affaires dans un contexte de forte croissance, articulation entre économie de la production et économie de la connaissance, hybridation des canaux de diffusion). S'y ajoutent des questions plus spécifiques soulevées par la force et la nature de la croissance des trans...

  17. Contrôle, financement et croissance des petites et moyennes entreprises

    OpenAIRE

    Anaïs HAMELIN

    2010-01-01

    Cette thèse explore les stratégies financières et organisationnelles adoptées par les petites et moyennes entreprises (PME) qui leur permettent de concilier croissance et indépendance. Les quatre chapitres apportent des contributions théoriques et empiriques à cette question. Le premier chapitre teste le lien entre contrôle familial et croissance sur un large échantillon de PME françaises. Le lien négatif entre contrôle familial et croissance s’explique par le fait que les entreprises familia...

  18. Algorithmes et architectures pour ordinateurs quantiques supraconducteurs

    Science.gov (United States)

    Blais, A.

    2003-09-01

    Algorithms and architectures for superconducting quantum computers Since its formulation, information theory was based, implicitly, on the laws of classical physics. Such a formulation is however incomplete because it does not take into account quantum reality. During the last twenty years, expansion of theory information to include quantum effects has known growing interest. The practical realization of a system for quantum data processing system, a quantum computer, presents however many challenges. In this book, we are interested in various aspects of these challenges. We start by presenting algorithmic concepts like optimization of quantum computations and geometric quantum computation. We then consider various designs and aspects of qubits based on Josephson junctions. In particular, an original approach to the interaction between superconducting qubits is presented. This approach is very general since it can be applied to various designs of qubits. Finally, we are interested in read-out of the superconductic flux qubits. The detector suggested here has the advantage that it is possible to uncouple it from the qubit when no measurement is in progress. Depuis sa formulation, la théorie de l'information a été basée, implicitement, sur les lois de la physique classique. Une telle formulation est toutefois incomplète puisqu'elle ne tient pas compte de la réalité quantique. Au cours des vingt dernières années, l'expansion de la théorie de l'information, de façon à englober les effets purement quantiques, a connu un intérêt grandissant. La réalisation d'un système de traitement de l'information quantique, un ordinateur quantique, présente toutefois de nombreux défis. Dans cet ouvrage, on s'intéresse à différents aspects concernant ces défis. On commence par présenter des concepts algorithmiques comme l'optimisation de calculs quantiques et le calcul quantique géométrique. Par la suite, on s'intéresse à différents designs et aspects de l

  19. Bouvard et Pécuchet et le savoir médical

    Directory of Open Access Journals (Sweden)

    Norioki Sugaya

    2009-01-01

    Full Text Available Le présent article a pour objet le dossier médical que Flaubert a constitué pour la préparation du chapitre III de Bouvard et Pécuchet. Ce dossier considérable, qui contient des notes de lecture prises par le romancier en vue de la rédaction du chapitre médical, nous permet de comprendre à quel point le « comique d’idées » propre au roman encyclopédique est le produit d’un travail intense sur le savoir. L’écriture romanesque de Bouvard explore en effet les configurations épistémologiques de chaque discipline, et met au jour surtout ses apories et ses insuffisances. Nous nous proposons donc d’aborder le dossier médical pour saisir le sens de l’excès documentaire pour l’esthétique flaubertienne. Notre étude se compose de trois parties. D’abord, nous étudierons la composition du dossier en nous demandant quels ouvrages médicaux a lu l’auteur de Bouvard. Ensuite, nous essaierons d’examiner le travail critique de Flaubert pratiqué sur les discours du savoir, en particulier la manière dont il traque et construit des contradictions. Enfin, nous analyserons un épisode du roman, celui de la fièvre typhoïde de Gouy, dont l’étude de la genèse nous montrera l’inscription de l’épistémologique dans la représentation romanesque.This article proposes to study the medical documents gathered by Flaubert for the preparation of Chapter III of Bouvart et Pécuchet. This substantial file, which contains reading notes taken by the novelist for the writing of the medical chapter, allows us to understand to what extent the “comic of ideas”, characteristic of the encyclopedic novel, is the result of an intensive work on knowledge. Indeed, the novelistic writing of Bouvard explores the epistemological configurations of each discipline, and in particular reveals its aporia and its insufficiencies. We have chosen to focus on the medical file in order to understand the meaning of the documentary excess with

  20. Théorie de la justice et idéologie : Hume et Rawls

    Directory of Open Access Journals (Sweden)

    Eléonore Le Jallé

    2008-04-01

    Full Text Available Les points d’accord entre Rawls et Hume vont au-delà du seul repérage des « circonstances de la justice ». Même si Hume se trouve attaché à la théorie utilitariste de l’impartialité que Rawls rejette, Hume n’est pas, selon Rawls, « à proprement parler » utilitariste : il a su reconnaître l’idée selon laquelle les institutions doivent fonctionner dans l’intérêt de chacun. L’idée d’une coopération sociale en vue de l’avantage mutuel est donc commune à ces deux auteurs. Ils partagent, en outre, une attitude similaire face au « problème de la confiance » en lien avec la stabilité de la justice : l’un et l’autre ajoutent ainsi à la solution extérieure qu’est l’institution étatique, un facteur intrinsèque de stabilité, à savoir, le sens de la justice. L’idée de « justice procédurale » et le rejet, corrélatif, d’un critère indépendant du juste, les relie également ; d’où, enfin, une certaine analogie des critiques respectivement adressées à ces deux auteurs.The specification of the “circumstances of justice” is not the only point on which Rawls agrees with Hume. If Rawls rejects the utilitarian theory of impartiality which is “reminiscent of Hume”, he does not consider him as “strictly speaking utilitarian”. Indeed, Rawls thinks that Hume has well understood that institutions have to be to the advantage of everyone. Besides, Hume and Rawls agree in thinking that the stability of justice involves a public sense of justice. Finally, the idea of “pure procedural justice” is shared by these authors: both have rejected any independent criterion for the right result, so that the ways they were criticized are rather similar.

  1. Comparative evaluation of energy-related aspects in urban and in rural districts - Case study in the Canton of Neuchatel, Switzerland; Analyse comparative des aspects energetiques de quartiers urbains et ruraux. Etude de cas dans le canton de Neuchatel. Rapport final

    Energy Technology Data Exchange (ETDEWEB)

    Perret, L.; Vuilleumier, R.; Bonhote, P. [Planair SA, La Sagne (Switzerland); Rey, E. [Bauart Architectes et Urbanistes SA, Neuchatel (Switzerland)

    2007-07-01

    The project aims at identifying the energy impact of various current ways of regional planning through a case study of eight districts in the canton of Neuchatel. The districts reveal the trends of planning in an urban centre, and in incorporated (suburb) or unincorporated territory. Thus, the influence of the distance to the centre of the agglomeration named 'centrality' has been studied. The energy expenditure for the buildings' construction phase (buildings, roads, networks) and utilization phase (heating, electricity, water, waste, networks) has been taken into account as well as the mobility. The energy investments have been evaluated by the method SNARC. The energy expenditure for operation and mobility has been evaluated based on questionnaires addressed to the inhabitants. As a result, the variable 'centrality' has only a weak role on the life cycle analysis (LCA) per inhabitant. In our case, the LCA for incorporated districts (suburb) and unincorporated districts are similar. With a LCA per square meter, the impact is completely similar and no correlation with the variable 'centrality' has been noticed. As the households are larger per inhabitants when the district is located in a suburban zone, the LCA per square meter remains stable. On the contrary, if the LCA is determined per household, the LCA is perfectly correlated with the variable 'centrality'. Thus, a household in the centre of Neuchatel consumes twice less energy resources than a household in Val-of-Ruz (rural area). The study highlights also the critical impacts of a LCA on a district scale. Finally, this study gives recommendations based on the energy and demographic prospects in order to respond to the objective of a 2000 watts society. (author)

  2. Religion et entertainment sont-ils compatibles?

    Directory of Open Access Journals (Sweden)

    Guy Marchessault

    2011-01-01

    Full Text Available Est-ce possible de réconcilier l’approche spectaculaire des médias avec la nature intérieure du spirituel? Peut-on imaginer la présence des religions dans une ambiance de divertissement? Il y a toujours eu tensions entre les religions et le jeu: elles craignaient le théâtre, les jeux, la musique, les arts, la danse, les jeux de cartes… et les médias, naturellement. Pourquoi l’entertainment fut-il considéré si dangereux? N’y aurait-il pas une approche plus positive qui permettrait de discerner les ouvertures vers le spirituel ressortant du jeu et de l’entertainment médiatique? Un historien, un réalisateur de film, des chercheurs en communication, un philosophe, plusieurs sociologues et anthropologues ont su raffiner la compréhension des capacités du jeu de révéler les humains à eux-mêmes dans leur recherche de signification et leur cheminement spirituel. Le jeu—et le divertissement médiatique—pourraient ouvrir les humains à leurs propres capacités “illimitées”, donnant signification à leurs relations au monde et aux autres humains, et par le fait même au sacré. Mais cela ne peut réussir que si chacun respecte les langages médiatiques: la narrativité et la façon de raconter. Ce qui implique une créativité artistique et rhétorique, combinée à un respect pour les dimensions éthiques et spirituelles des croyants.

  3. Response to Signorovitch et al.

    Science.gov (United States)

    Flot, Jean-François; Debortoli, Nicolas; Hallet, Bernard; Van Doninck, Karine

    2016-08-22

    Signorovitch et al.[1] comment that an Oenothera-like meiosis [2] could produce a pattern similar to what we observed in our study of natural isolates of the bdelloid rotifer Adineta vaga, which we attributed to horizontal gene transfers (HGTs) [3]. Indeed, our HGT hypothesis appears at first sight difficult to conciliate with their observation of a congruent pattern of allele sharing at four large loci possibly located on different chromosomes [4]. However, one might imagine conditions under which massive horizontal gene transfer between bdelloid individuals could produce such a pattern, notably if the individuals involved had previously lost most of their heterozygosity because of their exposure to frequent desiccation (which produces DNA double-strand breaks [5]). In the published A. vaga genome the loss of heterozygosity due to large-scale gene conversion events or break-induced replication covers only about 10% of the genome [6], but this percentage may be much higher in environmental isolates that often experience dessication. Besides, if an Oenothera-like mode of meiosis occurs in bdelloids frequently enough to be detected in a single sampling of 29 individuals (as in [4]), one would expect males and meiosis to be observed at least occasionally, and instances of congruent allele sharing across loci should turn up frequently in genetic surveys. This was not the case in [3]: among the 82 A. vaga individuals sequenced for four nuclear markers, no trio of individuals presented congruent patterns of shared sequences at different loci. For these reasons, and in the absence of any direct evidence for an Oenothera-like meiosis in bdelloids, we still consider inter-bdelloid HGTs a more parsimonious explanation for our results. PMID:27554651

  4. Uso de micorrizas e rizóbio em cultivo consorciado de eucalipto e sesbânia: II - Absorção e eficiência de utilização de fósforo e frações fosfatadas Use of mycorrhizas and rhizobium in intercropping system of eucalyptus and sesbania: II - Phosphorus uptake and efficiency of use and phosphate phosphate -fractions

    Directory of Open Access Journals (Sweden)

    L. A. Rodrigues

    2003-08-01

    Full Text Available Realizou-se um experimento em casa de vegetação com o objetivo de avaliar os efeitos de fungos micorrízicos arbusculares (FMAs e rizóbio na absorção e eficiência de utilização de P e nas frações fosfatadas em mudas de Eucalyptus grandis, cultivadas em consorciação com Sesbania virgata. Os tratamentos foram: inoculação ou não com FMAs em ambas as espécies de plantas e inoculação ou não com rizóbio na S. virgata, com quatro repetições. Ambas as plantas foram cultivadas em vasos de 6 L de capacidade, durante 100 dias, quando foram colhidas. A inoculação com FMAs ou FMAs + rizóbio aumentou o conteúdo de P no eucalipto, enquanto a inoculação com rizóbio, FMAs ou FMAs + rizóbio aumentou a eficiência de utilização de P. Nas frações de P, avaliadas nas folhas de eucalipto, observou-se aumento do fósforo total solúvel em ácido (PST nos tratamentos com inoculação de rizóbio ou FMAs + rizobio. Nos tratamentos com inoculação com rizóbio, FMAs, FMAs+rizóbio ou sem inoculação, observou-se que 81, 32, 91 e 68%, respectivamente, do PST foram encontrados como fósforo orgânico (Po. Em uma avaliação conjunta das frações fosfatadas e do conteúdo de P na parte aérea do eucalipto, o que aparentemente influenciou o aumento do PST e do Po não foi o conteúdo interno de P na planta, mas, sim, a inoculação do rizóbio na sesbânia.The effects of arbuscular mycorrhizal fungi (AMF and Rhizobium on P uptake and efficiency of use, as well as on the P fractions of Eucalyptus grandis grown in an intercropping system with Sesbania virgata were evaluated under greenhouse conditions. The treatments were: with or without inoculation with AMF of both plant species and with or without inoculation with Rhizobium of S. virgata plants only, in four replications. The two plant species were grown together in pots with a volume of 6 L for 100 days. Inoculations with AMF or with AMF + Rhizobium increased the P content in Eucalyptus

  5. Er strafferetten kun et offentligt anliggende?

    DEFF Research Database (Denmark)

    Feldtmann, Birgit

    2007-01-01

    Artikelen sætter fokus på spørgsmålet, på hvilke måder strafferetten er - eller kunne være - et anliggende for borgeren, fremfor alene være et anliggende for det statslige strafferetssystem....

  6. ETS transcription factors in embryonic vascular development.

    Science.gov (United States)

    Craig, Michael P; Sumanas, Saulius

    2016-07-01

    At least thirteen ETS-domain transcription factors are expressed during embryonic hematopoietic or vascular development and potentially function in the formation and maintenance of the embryonic vasculature or blood lineages. This review summarizes our current understanding of the specific roles played by ETS factors in vasculogenesis and angiogenesis and the implications of functional redundancies between them.

  7. Médiation organisationnelle et Droit

    OpenAIRE

    Bonifassi, Marie Helene

    2008-01-01

    Quels liens peut-on établir entre les phénomènes de dématérialisation des documents et l'apparition dans la sphère juridique d'un "soft law", ou "droit souple", caractérisé par le caractère largement contractuel et non coercitif ?

  8. Cassini's Grand Finale: The Final Orbits

    Science.gov (United States)

    Spilker, Linda; Edgington, Scott

    2016-04-01

    The Cassini-Huygens mission, a joint collaboration between NASA, ESA and the Italian Space Agency, is approaching its last year of operations after nearly 12 years in orbit around Saturn. Cassini will send back its final bits of unique data on September 15th, 2017 as it plunges into Saturn's atmosphere, vaporizing and satisfying planetary protection requirements. Before that time Cassini will continue its legacy of exploration and discovery with 12 close flybys of Titan in 2016 and 2017 that will return new science data as well as sculpt the inclinations and periods of the final orbits. Even though all of our close icy satellite flybys, including those of Enceladus, are now completed, numerous Voyager-class flybys (summer solstice approaches. In November 2016 Cassini will transition to a series of orbits with peripases just outside Saturn's F ring. These 20 orbits will include close flybys of some tiny ring moons and excellent views of the F ring and outer A ring. The 126th and final close flyby of Titan will propel Cassini across Saturn's main rings and into its final orbits. Cassini's Grand Finale, starting in April 2017, is comprised of 22 orbits at an inclination of 63 degrees. Cassini will repeatedly dive between the innermost rings and the upper atmosphere of the planet providing insights into fundamental questions unattainable during the rest of the mission. Cassini will be the first spacecraft to explore this region. These close orbits provide the highest resolution observations of both the rings and Saturn, and direct in situ sampling of the ring particles, composition, plasma, Saturn's exosphere and the innermost radiation belts. Saturn's gravitational field will be measured to unprecedented accuracy, providing information on the interior structure of the planet, winds in the outer layers of Saturn's atmosphere, and the mass distribution in the rings. Probing the magnetic field will give insight into the nature of the magnetic dynamo, telling us: why the

  9. Citoyenneté et éducation

    OpenAIRE

    Vally, Salim

    2011-01-01

    Cet article tente de comprendre la citoyenneté à la lumière de la pauvreté et des inégalités dans l’Afrique du Sud d’après l’apartheid. Il souligne le hiatus entre la noblesse des déclarations officielles et la réalité. Il examine les politiques éducatives, la législation et les programmes scolaires structurés par les idéaux des droits de l’homme, la justice sociale et le discours démocratique citoyen et les confronte à leur réalisation concrète dans un contexte néolibéral de mondialisation. ...

  10. Cartographie et éducation populaire

    OpenAIRE

    Ferretti, Federico

    2012-01-01

    Cet article aborde la constitution du Musée Cartographique de la Ville de Genève, ouvert entre 1907 et 1922 à partir du fonds cartographique de Charles Perron et d’Élisée Reclus. À travers l’analyse de sources édites et inédites, nous reconstruisons cette expérience en nous interrogeant sur le rapport entre géographie et éducation populaire, et sur le rôle de la cartographie dans la démarche de ces géographes. Reclus, histoire de la cartographie, histoire de l’éducation géographique Este a...

  11. Ivan Axionov/Picasso et alentours

    OpenAIRE

    Payraudeau, Amaury

    2012-01-01

    Poète novateur et membre du Futurisme, Ivan Axionov rend ici justice à Pablo Picasso qu’il admire et qu’il souhaite laver des accusations de mysticisme et de « peintre de musée ». En partant d’une définition de l’art qui fait la part belle au rythme, ce contemporain de Picasso, digne représentant d’une Russie cosmopolite et européenne, retranscrit le parcours du peintre espagnol, son intérêt pour la sculpture et l’influence qu’elle a eue sur sa peinture. Détaillant, par exemple, les technique...

  12. Introduction : Gouvernance et grande vitesse

    OpenAIRE

    Belot, Robert

    2015-01-01

    On peut dire que la grande vitesse ferroviaire a été une « révolution » dans la mesure où elle a permis d’appréhender le ferroviaire autrement que comme un simple moyen de transport et d’accroissement de la mobilité. Elle a été regardée et appropriée par les politiques comme une aubaine de transformation et de dynamisation des territoires, comme une promesse de dépassement des frontières et d’accélération du processus d’européanisation, comme une assurance d’égalité sociale et territoriale ma...

  13. Service de capitalisation et diffusion de connaissances

    Directory of Open Access Journals (Sweden)

    Vincent Baron

    2007-07-01

    Full Text Available L’article relate l’expérience de mise en place d’un service de capitalisation et diffusion des connaissances au sein d’un établissement de recherche. Plus de 2000 documents, traitant de la thématique « Agricultures Familiales et Mondes à venir », sont accessibles en texte intégral. La démarche présentée est axée sur la co-construction de concepts (ontologie et l’élaboration d’une indexation des mots clés sous forme graphique (cartographie sémantique. L’association des deux démarches présente de nombreux intérêts et mérite d’être approfondie pour améliorer la mutualisation des connaissances au sein des collectifs de recherche et leur diffusion.

  14. Ontologie et base de connaissances pour le pré-traitement et post-traitement en fouille de données

    OpenAIRE

    BRISSON, Laurent; Collard, Martine; Pasquier, Nicolas

    2006-01-01

    National audience; Le Système d'Information Conceptuel ExCIS pour l'extraction de connaissances est une approche s'inspirant de CRISP- DM et intégrant le support des ontologies. Il permet de définir une ontologie de représentation des connaissances expertes du domaine, prenant en compte les besoins de la fouille de donnée, afin d'améliorer la pertinence des modèles de connaissances extraits et d'assister l'utilisateur final dans l'exploitation de ces modèles. Les connaissances expertes du dom...

  15. Didactiques scolaires et didactiques non scolaires, pedo- et andro-didactiques... : le cas des didactiques des langues et du FLS

    OpenAIRE

    Bouchard, Robert

    2009-01-01

    1° colloque international de l'ARCD, Genève, janvier 2009: , " Où va la didactique comparée ? Didactiques disciplinaires et approches comparatistes des pratiques d'enseignement et d'apprentissage " International audience Les didactiques des disciplines sont souvent des didactiques scolaires. Pour élargir ses perspective, la comparaison en didactique doit prendre en compte d'autres publics et d'autres institutions. C'est ce que peut favoriser la didactique des langues dont l'histoire est...

  16. The reconstruction of jets, missing ET and boosted heavy particles with ATLAS in Run II

    CERN Document Server

    Santoni, Claudio; The ATLAS collaboration

    2015-01-01

    The reconstruction of jets, missing ET and boosted heavy particles decaying hadronically has proved to be of extreme importance in Run 1 of the LHC, and has great potential to uncover new physics with Run 2 data. ATLAS has implemented and commissioned several new techniques for the analysis and interpretation of hadronic final states at the LHC. These include event-by-event pile-up subtraction algorithms for jets and missing ET, jet substructure, quark-gluon discrimination, and jet tagging tools for the identification of boosted heavy particles. The excellent ATLAS detector capabilities, in particular its high resolution longitudinally segmented calorimeter and inner detector, have enabled the development of complex clustering and calibration algorithms for the reconstruction of jets, missing ET, and jet substructure, and its validation and calibration in data using large datasets collected during 2012. A summary of the most modern jet, missing ET, and jet substructure and tagging tools developed in ATLAS, an...

  17. Etablissement et évolution des interfaces lors du soudage diffusion

    OpenAIRE

    Rigal, Emmanuel; Bouquet, Nicolas; Bernacki, Marc; Bernard, Frédéric

    2015-01-01

    La genèse des microstructures des interfaces soudées diffusées implique plusieurs mécanismes qui ont lieu simultanément et dont le développement au cours du cycle de soudage dépend des paramètres appliqués et des propriétés des surfaces initiales. L'élimination des pores est obtenue par la plastification immédiate des aspérités sous l'effet de la pression, leur fluage et finalement la diffusion. Certains alliages sont très sensibles à l'oxygène résiduel et forment facilement des oxydes ou des...

  18. Response to Delibes-Mateos et al. : Pellet size matters

    Science.gov (United States)

    Rueda, Marta; Rebollo, Salvador; Gálvez-Bravo, Lucía

    2009-05-01

    In Rueda et al. [Rueda, M., Rebollo, S., Gálvez-Bravo, L., 2008. Age and season determine European rabbit habitat use in Mediterranean ecosystems. Acta Oecol. 34, 266-273] we used a threshold of 6 mm faecal pellet diameter to differentiate between adult and juvenile European rabbit ( Oryctolagus cuniculus) habitat use. Delibes-Mateos et al. designed a housing experiment with 12 adult rabbits and criticised the choice of 6 mm as a threshold to separate adult and juvenile rabbit pellets, claiming that adults can produce pellets both larger and smaller than 6 mm in similar proportions. In response to their criticism we argue the following. The selection of a 6 mm threshold has a bibliographic basis, it is not a new method developed by Rueda et al. and produces consistent results when applied in the field. Assuming that Delibes-Mateos et al. results are accurate, we should have found a greater number of 6 mm, overall and seasonally, which is not the case. We believe that the use of commercial pelleted food, keeping animals isolated in small cages for over a year, and the use of adult rabbits only, makes the experimental design used by these authors not suitable to refute the usefulness of separating rabbit pellets smaller and larger than 6 mm diameter as indicators of changes in the relative abundance of juvenile and adult rabbits in the field. Finally, we agree with the authors that the use of indirect methods of animal aging would require case-specific validation studies; however, we believe these studies should be correctly designed.

  19. Le marquis de Pombal et la politique scientifique du Portugal

    Directory of Open Access Journals (Sweden)

    Henrique Leitão

    2011-08-01

    Full Text Available Les réformes éducatives réalisées par le marquis de Pombal (1699-1782 au milieu du xviiie siècle sont généralement considérées comme un des moments critiques dans la genèse d’une politique scientifique moderne au Portugal. À la suite de l’expulsion de la Compagnie de Jésus (1759, la structure de l’enseignement secondaire et pré-universitaire a été radicalement transformée. Avec la réforme de l’Université (1772, la définition même d’enseignement supérieur et le rôle de l’université ont été profondément altérés. Le programme réformateur de Pombal était explicitement modernisateur (introduction des sciences expérimentales, création d’une Faculté de mathématiques, abandon du latin, etc., et il a été conduit avec la détermination de la personnalité excessive du célèbre marquis. Mais en dépit de l’indéniable modernité de cet ambitieux projet, les actions de Pombal étaient aussi très influencées par des contraintes politiques et une vision particulière du rôle de la science dans la société. Le résultat final, selon plusieurs historiens, a été extrêmement complexe, voire paradoxal.

  20. Fiction et forme encyclopédique : Wookieepedia, Dragon Ball Wiki et Cie.

    Directory of Open Access Journals (Sweden)

    Benoît Berthou

    2011-06-01

    Full Text Available Wookieepedia, Final Fantasy Wiki, Dragon Ball Wiki, Memory Alpha, WoWWiki… Autant d’encyclopédies en ligne abordant de célèbres fictions pour la jeunesse à travers un nombre parfois considérable d’articles abordant toutes sortes de sujets (récits, technologies, personnages, lieux, auteurs, fans…. Que nous apprennent ces dispositifs entendant prendre des fictions au sérieux, c’est-à-dire aborder un domaine relevant de l’imaginaire avec des outils relevant du domaine de la connaissance ? Deux pratiques se dégagent : la référence à des produits culturels prenant des formes fort diverses (romans, bandes dessinées, films, jeux vidéo… et le lien entre les divers articles. Ces deux pratiques montrent comment se développe une expertise quant à une fiction, une capacité à se situer dans une construction imaginaire.

  1. Characterization of Rhizobial Isolates of Phaseolus vulgaris by Staircase Electrophoresis of Low-Molecular-Weight RNA

    OpenAIRE

    Velázquez, Encarna; Martínez-Romero, Esperanza; Rodríguez-Navarro, Dulce Nombre; Trujillo, Martha E.; Daza, Antonio; Mateos, Pedro F.; Martínez-Molina, Eustoquio; Van Berkum, Peter

    2001-01-01

    Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum bv. viciae and bv. trifolii, and Sinorhizobium fredii.

  2. Etude des defauts microscopiques et des proprietes optiques, electroniques et magnetiques du compose neodyme cerium oxyde de cuivre

    Science.gov (United States)

    Richard, Pierre

    La presente these, qui combine des mesures de diffusion Raman, de transmission infrarouge, de conductivite hyperfrequence et d'interferometrie ultrasonore sur les composes Nd 2-xCexCuO 4, traite des defauts d'oxygene, ainsi que des proprietes optiques, electroniques et magnetiques de ces materiaux. Les resultats experimentaux obtenus sont correles avec plusieurs donnees experimentales disponibles dans la litterature. D'abord, la caracterisation des modes Raman et des niveaux d'energie de champ cristallin de l'ion Nd3+ au moyen de techniques optiques, en fonction du dopage en cerium et du contenu en oxygene, permettent de conclure a la presence de defauts lies a une non-stoechiometrie en oxygene dans ces composes. En effet, des excitations de champ cristallin assignees a des ions Nd3+ en sites irreguliers sont observees, en plus des bandes d'absorption associees aux ions Nd3+ en site regulier, dans les spectres correspondant aux multiplets 4I11/2, 4I13/2 et 4I 15/2 de l'ion Nd3+. Les resultats experimentaux indiquent que, contrairement a la croyance largement repandue, les oxygenes apicaux, bien que presents dans les echantillons dopes, ne sont pas enleves lors du processus de reduction des echantillons necessaire pour faire apparaitre la supraconductivite dans cette famille de cuprates. Au contraire, des lacunes d'oxygene, dont le type varie en fonction du dopage, sont creees lors de ce processus. En particulier, il est montre dans ce travail que la reduction des echantillons dopes de maniere optimale conduit a la creation de lacunes d'oxygene dans les plans CuO2. Les consequences de telles lacunes sont largement discutees. En outre, il est suggere que de telles lacunes sont responsables de la perte de l'ordre antiferromagnetique a longue portee des ions Cu2+. Finalement, l'interaction d'echange anisotrope Nd3+-Cu 2+ dans le compose nondope est caracterisee au moyen de la transmission infrarouge sous champ magnetique. L'eclatement des doublets de Kramers mesure

  3. Vents et nuages la physique du ciel

    CERN Document Server

    2013-01-01

    Les nuages et les vents sont au cœur des attentions des climatologues et des météorologues. Les premiers s’intéressent à leurs interactions avec le réchauffement climatique. Les seconds cherchent à prédire le temps qu’il fera demain, mais aussi les manifestations extrêmes (tornades, orages, cyclones…). Un numéro pour rester le nez au vent et la tête dans les nuages !

  4. Les fonctionnaires entre association et syndicat

    OpenAIRE

    Siwek-Pouydesseau, Jeanne

    2015-01-01

    Jeanne Siwek-Pouydesseau Dans l'administration, le groupement professionnel revendicatif a d'abord été un fait social, bien avant d'être juridiquement reconnu et institutionnalisé. On parlera donc, ici, des syndicats au sens sociologique et politique, même en l'absence de reconnaissance juridique. Seront plus spécifiquement évoqués les fonctionnaires de l'État, qui étaient au centre du problème. La particularité des syndicats de fonctionnaires est d'avoir dû attendre et se battre bien plus lo...

  5. Når E'et forsvinder

    DEFF Research Database (Denmark)

    Levinsen, Karin

    2010-01-01

    E-læring - et begreb under afvikling? E-learning har længe været et buzzword, men om nogle år vil e'et måske forsvinde fra e-learning og dermed fra e-vejledning, fordi vejledning uden inddragelse af it vil høre fortiden til. Hvad er det egentlig for en digital virkelighed, vi er i? Og hvordan kan...... vi skabe en digital vejledning, så den passer til den virkelighed og til de unge?...

  6. Écriture et processus de transformation

    OpenAIRE

    Hillion, Jacques

    2010-01-01

    Dissertação de Mestrado apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Psicopedagogia Perceptiva. Ce mémoire relate et analyse un processus expérimental : une série d’ateliers d’écriture est proposée à des praticiens en psychopédagogie perceptive ayant des difficulté à tenir et utiliser leur journal de bord. En analysant de manière qualitative questionnaires et journaux de bord, cette recherche met en relief l’impact de ces...

  7. Eaux, pauvreté et crises sociales

    OpenAIRE

    Abdedayem, Slahedine; Abu Dayyeh, Qasem; Aidara, Cheikhna A.; Angueletou, Anastasia; Atif Kishk, Mohammed; Aubriot, Olivia; Ayeb, Habib; Bashir, Basema; Sylvia I Bergh; Jacques BETHEMONT; Blanchon, David; Bouchaou, Lhoussaine; Bouchelkha, Mohammed; Bouquet, Christian; Cardoso, Ana Claudia Duarte

    2013-01-01

    Au Nord comme au Sud, l’accès à l’eau, que celle-ci soit à usage domestique ou agricole, devient de plus en plus difficile pour les populations pauvres, alors que les différents types d’exclusion se combinent pour accentuer les inégalités. Pour comparer les processus actuellement en cours sur les cinq continents et tenter d’inverser ces tendances, l’IRD a organisé en 2005 des rencontres à l’université d’Agadir avec l’appui du CRDI et de la Ford Fondation. Les chercheurs, décideurs et responsa...

  8. Data used in Xu et al., 2016

    Data.gov (United States)

    U.S. Environmental Protection Agency — Mercury emissions data from anthropogenic sources as described in Xu et al., 2016. This dataset is associated with the following publication: Xu, X., N. Liu, M....

  9. Recherche et développement

    Directory of Open Access Journals (Sweden)

    Icart Jean-Claude

    2000-03-01

    Full Text Available La présence des sciences sociales au centre de ce dossier, Afrique, plantation et développement, n’est pas seulement significative d’une situation propre au problème du développement des pays du Sud, et de l’Afrique en particulier. Les perspectives, au Nord, d’une agriculture multifonctionnelle, plus sensible aux dimensions territoriales et sociales des ses activités, confrontent la recherche agronomique à des problèmes similaires de médiation, liée aux mêmes impératifs de conciliation entre démarche scientifique et action, imposant le même recours aux analyses sur le milieu socio-économique.

  10. Barbare et infidèle 

    OpenAIRE

    Platov, Ilya

    2012-01-01

    La compréhension de la mobilisation patriotique des Russes en faveur de la libération des Slaves du Sud au cours de la crise balkanique et de la guerre russo-turque de 1876-1878 nécessite la prise en compte des récits de cruautés relayés par la presse et par d’autres vecteurs d’information qui aboutissent à la cristallisation d’une représentation de l’ennemi à la fois barbare et infidèle. Pendant plus de deux ans, la population russe est soumise à une véritable surenchère d'images et de récit...

  11. Sport et masculinités

    OpenAIRE

    McKay, Jim; Laberge, Suzanne

    2008-01-01

    Cet article examine le régime sexuel du sport en se fondant sur des recherches récentes portant sur les hommes et les masculinités. Malgré le caractère tenace des liens entre les hommes, les masculinités et le sport, nous croyons que le sport constitue un contexte idéal pour « étudier par le haut », comme le propose Connell (1990), l'ordre hiérarchique de genre. Cinq secteurs de recherche sont abordés : les organisations sportives, les corps et le modèle de « puissance et performance » du spo...

  12. Et saada riigireformi 1. juuliks / Mart Raudsaar

    Index Scriptorium Estoniae

    Raudsaar, Mart, 1973-

    2016-01-01

    Võro Seltsi ettepanekust, et üheks võiks liituda kõik Vana Võromaa territooriumile jäävad 25 omavalitsust ning Vana Võromaa kunagised kaheksa kihelkonda moodustaksid uue omavalitsuse osavallad

  13. Musée et technique

    OpenAIRE

    Mairot, Philippe

    2007-01-01

    « Mais derrière les merveilles de Florence je n'arrive pas à oublier Calimala, l'arte della lana, l'arte della seta, le suint et la poussière, le cuveau à teinture et la terre à foulon, l'humidité de cave, la saleté ténébreuse de la manufacture. Derrière les tableaux de Giotto et de Botticelli, malgré moi surgit en surimpression Tourcoing, et c'est assez pour que cet art miraculeux, de quelque façon que je me raisonne, garde quelque chose d'une fleur de fumier, d'une beauté née dans la crotte...

  14. Lait et produits laitiers en Adamaoua

    OpenAIRE

    Boutrais, Jean

    2013-01-01

    Grande région d’élevage, l’Adamaoua est également supposé être une grande région productrice de lait. En fait, deux systèmes d’élevage y coexistent et ils se différencient par un rôle économique et culturel très inégal du lait. L’opposition entre élevages foulbé et mbororo tient aux gestions techniques et spatiales du bétail, de même qu’à une valorisation de la viande bovine ou du lait. Des exemples d’utilisation du lait par des familles, après la traite, montrent une priorité à l’autoconsomm...

  15. E-Gov Travel (ETS) Measures

    Data.gov (United States)

    General Services Administration — The Number of Vouchers Processed measure represents the number of vouchers processed by the three ETS vendors Annual for the 24 largest Federal Civilian Agencies....

  16. Bæredygtighed i et arkitektonisk helhedssyn

    DEFF Research Database (Denmark)

    Sattrup, Peter Andreas

    2008-01-01

    Introduktion af Dietmar Eberles arkitekturteori: Arkitektur bør tænkes og formes efter et hierarki, der afspejler elementernes relative permanens: Stedet, Strukturen, Klimaskærmen, Programmet og Overfladerne....

  17. Musique et littérature

    OpenAIRE

    Arfouilloux, Sébastien; Aron, Paul; Beyen, Roland; Brogniez, Laurence; Curreri, Luciano; Detemmerman, Jacques; De Haes, Frans; Douche, Sylvie; Dozo, Björn-Olav; Fratta, Anna Soncini; Fréché, Bibiane; Gillain, Nathalie; Glinoer, Anthony; Goriely, Serge; Halen, Pierre

    2013-01-01

    Au cours de l’histoire des lettres belges (et, plus largement, européennes), la musique a parfois été promue au rang de modèle et parée de vertus diverses. Ce dossier explore trois moments particulièrement féconds de l’histoire croisée de la musique et de la littérature. Moments qui peuvent être résumés par trois notions clés, qui sont autant de fonctions accordées à la musique : la suggestion, qui caractérise la période symboliste ; le bouleversement, privilégié par les surréalistes ; et l’i...

  18. Pascal et le cœur

    OpenAIRE

    McKenna, Antony

    2016-01-01

    Un des problèmes les plus complexes dans l’interprétation des Pensées est la définition du « sentiment » et du « cœur » : certes on peut être sensible aux résonances bibliques et augustiniennes de ces termes tels qu’ils sont employés par Pascal, mais des difficultés considérables subsistent, comme l’a souligné récemment Henri Gouhier. En particulier, on comprend mal comment le cœur peut être le siège de sentiments tels que l’amour et la foi, et aussi l’instrument par lequel l’homme appréhende...

  19. La grammaire autrement sensibilisation et pratique

    CERN Document Server

    Chalaron, Marie-Laure

    1990-01-01

    Le public : niveau débutant. Les objectifs : mettre en évidence, faire réfléchir et faire produire sur les points de langue essentiels. Le contenu : des montages attractifs, des exercices de manipulation, des activités créatives, des feuilles volantes avec des bribes de textes à décomposer et à recomposer dans une perspective communicative. Un corrigé des exercices.

  20. André Gide et son Journal

    NARCIS (Netherlands)

    Albarda, Maria

    1942-01-01

    Nous croyons pouvoir dire, en terminant, gue c'est la musique, et Ia musique seule, qui a influencé Gide sans aucune interruption. Ses conceptions musicales n'ont pas varié. Il ne s'agit pas de considérations vagues sur les rapports de la musique et de la littérature: c'est la musique prise en soi q