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  1. Rh Factor Blood Test

    Science.gov (United States)

    Tests and Procedures Rh factor blood test By Mayo Clinic Staff Rhesus (Rh) factor is an inherited protein found on the surface of ... If your blood has the protein, you're Rh positive. If your blood lacks the protein, you' ...

  2. The prevalence of transfusion transmitted infections in ABO blood groups and Rh type system

    Directory of Open Access Journals (Sweden)

    Jitendra Singh Nigam

    2014-12-01

    Full Text Available Screening of blood and blood products is important to reduce the risk of transfusion transmitted infections (TTIs. The transfusion of unscreened or inadequately screened blood and blood products are the major source of TTIs. The aim of this paper is to find out the prevalence of TTIs in ABO blood groups and Rh type system. A total of 4128 blood donors were screened from January 2010 to April 2014. Serological tests were performed for hepatitis B surface antigen (HBsAg, anti hepatitis C virus (Anti-HCV, anti HIV-1 and 2, venereal disease research laboratory test (VDRL and malaria parasite (MP antigen. In seroreactive donors, HBsAg, Anti-HCV, VDRL, MP antigen and anti HIV were positive in 40 cases, 26 cases, 19 cases, 6 cases and 2 cases, respectively. Highest percentage of HBsAg, Anti HCV, VDRL, MP antigen and anti HIV was observed in blood group A negative (2/50, O negative (1/66, B negative (1/91, AB positive (2/377 blood group respectively. In the present study, the total number of Rh-negative donors is lower when compared to Rh-positive blood donors, but Rh-negative blood donors show higher percentages of seroreactivity for TTIs. Larger scale studies at molecular level are required to improve the knowledge of this aspect.

  3. The Prevalence of Transfusion Transmitted Infections in ABO Blood Groups and Rh Type System

    Science.gov (United States)

    Nigam, Jitendra Singh; Singh, Savitri; Kaur, Viplesh; Giri, Sumit; Kaushal, Ravi Prakash

    2014-01-01

    Screening of blood and blood products is important to reduce the risk of transfusion transmitted infections (TTIs). The transfusion of unscreened or inadequately screened blood and blood products are the major source of TTIs. The aim of this paper is to find out the prevalence of TTIs in ABO blood groups and Rh type system. A total of 4128 blood donors were screened from January 2010 to April 2014. Serological tests were performed for hepatitis B surface antigen (HBsAg), anti hepatitis C virus (Anti-HCV), anti HIV-1 and 2, venereal disease research Laboratory test (VDRL) and malaria parasite (MP) antigen. In seroreactive donors, HBsAg, Anti-HCV, VDRL, MP antigen and anti HIV were positive in 40 cases, 26 cases, 19 cases, 6 cases and 2 cases, respectively. Highest percentage of HBsAg, Anti HCV, VDRL, MP antigen and anti HIV was observed in blood group A negative (2/50), O negative (1/66), B negative (1/91), AB positive (2/377) blood group respectively. In the present study, the total number of Rhnegative donors is lower when compared to Rh-positive blood donors, but Rh-negative blood donors show higher percentages of seroreactivity for TTIs. Larger scale studies at molecular level are required to improve the knowledge of this aspect. PMID:25568761

  4. The Prevalence of Transfusion Transmitted Infections in ABO Blood Groups and Rh Type System.

    Science.gov (United States)

    Nigam, Jitendra Singh; Singh, Savitri; Kaur, Viplesh; Giri, Sumit; Kaushal, Ravi Prakash

    2014-11-19

    Screening of blood and blood products is important to reduce the risk of transfusion transmitted infections (TTIs). The transfusion of unscreened or inadequately screened blood and blood products are the major source of TTIs. The aim of this paper is to find out the prevalence of TTIs in ABO blood groups and Rh type system. A total of 4128 blood donors were screened from January 2010 to April 2014. Serological tests were performed for hepatitis B surface antigen (HBsAg), anti hepatitis C virus (Anti-HCV), anti HIV-1 and 2, venereal disease research Laboratory test (VDRL) and malaria parasite (MP) antigen. In seroreactive donors, HBsAg, Anti-HCV, VDRL, MP antigen and anti HIV were positive in 40 cases, 26 cases, 19 cases, 6 cases and 2 cases, respectively. Highest percentage of HBsAg, Anti HCV, VDRL, MP antigen and anti HIV was observed in blood group A negative (2/50), O negative (1/66), B negative (1/91), AB positive (2/377) blood group respectively. In the present study, the total number of Rhnegative donors is lower when compared to Rh-positive blood donors, but Rh-negative blood donors show higher percentages of seroreactivity for TTIs. Larger scale studies at molecular level are required to improve the knowledge of this aspect.

  5. A STUDY OF DISTRIBUTION OF ABO AND RH BLOOD GROUPS SYSTEM AMONG BLOOD DONORS AT A TERTIARY CARE HOSPITAL

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    Rajesh Kumar

    2015-02-01

    Full Text Available Up till now about 400red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rh blood group system was first to be identified and is most important for blood transfusion purposes. OBJECTIVE: This study was conducted to determine the frequency of ABO and Rhesus (Rh blood groups in a tertiary care teaching hospital in India. MATERIALS AND METHODS: A retrospective data based study was conducted at blood bank , Chirayu Medical College and Hospital, Bhopal, Madhya Pradesh, India over a period of four years. RESULTS: Study includes a record of 3188 (28.54% voluntary and 7982 (71.46% replacement donors attending blood bank from February 2011 to January 2015. Out of 11170, 10723(95.998% were male and 447(4.002% female donors. The most common blood group was found to be B in 4013 (35.927% donors followed by O in 3462 (30.994% donors , an in 2516 (22.524% donors and AB in 1179 (10.555% donors. Out of these, 10659(95.425% donors were Rh - positive while 511 ( 4.575 % were Rh - negative.

  6. The abundance and organization of polypeptides associated with antigens of the Rh blood group system.

    Science.gov (United States)

    Gardner, B; Anstee, D J; Mawby, W J; Tanner, M J; von dem Borne, A E

    1991-06-01

    Twelve murine monoclonal antibodies, which react with human red cells of common Rh phenotype but give weak or negative reactions with Rh null erythrocytes, were used in quantitative binding assays and competitive binding assays to investigate the abundance and organization of polypeptides involved in the expression of antigens of the Rh blood group system. Antibodies of the R6A-type (R6A, BRIC-69, BRIC-207) and the 2D10-type (MB-2D10, LA18.18, LA23.40) recognize related structures and 100,000-200,000 molecules of each antibody bind maximally to erythrocytes of common Rh phenotype. Antibodies of the BRIC-125 type (BRICs 32, 122, 125, 126, 168, 211) recognize structures that are unrelated to those recognized by R6A-type and 2D10-type antibodies and between 10,000 and 50,000 antibody molecules bind maximally to erythrocytes of the common Rh phenotype. The binding of antibodies of the R6A-type and the 2D10-type, but not of antibodies of the BRIC-125-type could be partially inhibited by human anti-D antibodies (polyclonal and monoclonal) and a murine anti-e-like antibody. These results are consistent with evidence (Moore & Green 1987; Avent et al., 1988b) that the Rh blood group antigens are associated with a complex that comprises two groups of related polypeptides of M(r) 30,000 and M(r) 35,000-100,000, respectively, and suggest that there are 1-2 x 10(5) copies of this complex per erythrocyte. The polypeptide recognized by antibodies of the BRIC-125 type is likely to be associated with this complex.

  7. Phenotypic Profile of Rh and Kell Blood Group Systems among Blood Donors in Cote d'Ivoire, West Africa

    Science.gov (United States)

    Siransy Bogui, L.; Dembele, B.; Sekongo, Y.; Abisse, S.; Konaté, S.; Sombo, M.

    2014-01-01

    Few countries in sub-Saharan Africa make systematic searches for antigens C, c, E, and e of the Rh and Kell system antigens in the donor and recipient, thereby exposing transfused patients. Purpose and Objectives. In this paper, we propose to determine the red cell Rh and Kell blood groups among blood donors from traditional techniques to improve medical care of transfused patients. This study will allow us to assess the frequency of blood group antigens in these systems. Study Design and Methods. We carried out a study on the red cell typing in the blood donor population of the National Blood Transfusion Center in Abidjan. This study was performed on 651 blood donors. Results. For the Rh system, the antigen frequencies of D, c, e, C, and E are, respectively, 92.93%, 99.85%, 99.85%, 21.97%, and 13.82%. K antigen is found in 0.77% of donors. Discussion and Conclusion. Although the frequencies of the most immunogenic antigens are lower than in the white race, lack of preventive measures makes the immunological risk high in Africa. Furthermore, Africa is full of specificities that are important to note for a better care of our patients. PMID:25328758

  8. Molecular basis of Rh blood group system in the Malaysian population.

    Science.gov (United States)

    Musa, Rozi Hanisa; Muhamad, Nor Asiah; Hassan, Afifah; Ayob, Yasmin; Yusoff, Narazah Mohd

    2015-01-01

    Rh molecular studies have been previously mainly conducted in Caucasians and African population. There is a limited data on the molecular basis for Rh genotypes among Asians. This study aims to characterize the Rh genes and frequency of the various RH genotypes among blood donors in National Blood Centre (NBC), Kuala Lumpur. A total of 1014 blood samples were obtained from blood donors from four different ethnic groups (360 Malays, 434 Chinese, 164 Indians and 56 others). Serological and molecular analysis of all 1014 blood samples were performed. An automated deoxyribonucleic acid sequencing analysis was performed. Rh phenotypes and RH genotypes showed heterogeneity and significant association with ethnicities. Discrepancies in allele D, C/c and E/e between phenotypes and genotypes results were observed. Discrepancy results in allele D showed significant association with the ethnic groups of the blood donors in NBC. There were multiple novel mutations (23) and published mutations (5) found in this study. Significant associations between discrepancy results and mutations were found in allele D and C/c. Performing RH molecular analysis in Malaysian population provided the basic database for the distribution of Rh genotypes of donors from major ethnic groups in Malaysia.

  9. Phenotypic and allelic profile of ABO and Rhésus D blood group system among blood donor in Antananarivo.

    Science.gov (United States)

    Randriamanantany, Z A; Rajaonatahina, D H; Razafimanantsoa, F E; Rasamindrakotroka, M T; Andriamahenina, R; Rasoarilalamanarivo, F B; Hanitriniala, S P; Herisoa, F R; Rasamindrakotroka, A; Rakoto Alson, O A

    2012-12-01

    This study assessed the phenotypic and allelic profiles of ABO and Rhesus D blood group system among first time blood donors at the National Centre of Blood Supply of Antananarivo. We collected through this retrospective study all data registered during 7 years of practice (from 2003 to 2009). Age and sex were analysed with the result of ABO and RhD screening. They were tested both with Beth Vincent and Simonin tests which were performed in a plate, by using commercial monoclonal antibody (Diaclone(®) et Eryclone(®)), and home-made red cells tests. The Rh D was performed with the same commercial kits. The frequencies of alleles were calculated by using Bernstein method. Data about 45,857 donors were obtained. A male predominance (80.46%) was found and most of our donors were aged ABO antigen was, respectively, 22.61, 29.66, 6.13 and 41.60% for A, B, AB and O antigen. Allelic frequencies of A, B and O were 0.1559, 0.1987 and 0.6454. These results confirmed the fact that Madagascan population had admixed ethnic origin.

  10. The screening and clinical significance of Rh blood group system%Rh血型系统阴性血型筛查及其临床意义

    Institute of Scientific and Technical Information of China (English)

    毕红琳; 刘更夫; 龚永启; 左芳; 郑璐琳

    2011-01-01

    Objective To investigate and analyze the RhD( — ) ,RhE( — ) and RhC( — ) of Rh blood group system and to provide the experiment basis for preventing hemolytic transfusion reaction(HTR) and newborn hemolytic disease(NHD). Methods 11 035 cases of blood samples were examined for ABO,RhD,RhE and RhC blood groups by using micro-column gel method. Results A mong all of the detected samples,0. 21% (23/11 035) were with RhD( — ),47. 8%(5 275/11 035)with RhE( — ) and 7. 6% (839/11 035) with RhE( — ). Conclusion To take Rh blood group system,including RhD,RhE and RhC,as routine blood group tests might have important clinical significance in the prevention of HTR and NHD,as well as in the improvement of the safety of a blood transfusion treatment.%目的 筛查Rh血型系统Rh(D)、Rh(E)、Rh(C)阴性血型,为临床预防溶血性输血反应及新生儿溶血性疾病的发生提供实验依据.方法 用微柱凝胶法对11 035例作ABO血型鉴定标本同时进行Rh血型系统Rh(D)、Rh(E)、Rh(C)阴性血型筛查.结果 Rh(D)抗原阴性23例,检出率为0.21%;Rh(E)抗原阴性5 275例,检出率为47.8%;Rh(C)抗原阴性839例,检出率为7.6%.结论 将Rh血型系统Rh(D)、Rh(E)、Rh(C)纳入到血型的常规检验项目,对预防溶血性输血反应的发生、提高输血治疗的安全性,以及预防新生儿溶血性疾病的发生具有十分重要的临床意义.

  11. [Evaluation of blood grouping in ABO and Rh systems in health facilities in Benin].

    Science.gov (United States)

    Anani, L Y; Lafia, E; Ahlonsou, F; Sogbohossou, P; Bigot, A; Fagbohoun, J; Meton, A; Adjaka, A; Latoundji, S; Py, J-Y; Zohoun, I S

    2014-05-01

    The goal of this work is to assess the modalities of blood typing achievement in Benin with the view of their improvement. On the basis of a questionnaire including the detailed operative process, a prospective investigation has been achieved in public and private health centers laboratories. It came out that the execution of ABO and Rh blood typing took place globally on the fringe of the standards. We note that 72.4% of the private laboratories and 48.9% of the public ones lacked at least one equipment and 51.3% at least one material for blood withdrawal; 38.2% of the laboratories did not respect blood withdrawal standards; 1.32% of the laboratories applied the 4×2 rule. The assessment revealed that respectively 10.8% and 30.7% of the blood centers and non-blood centers achieved the globular test solely; the same 40.5% and 46.2% used reagents of different brands. Anti-A1 and anti-H sera, and A1 and A2 red cells were not available in any laboratory. More than 64% of laboratories have senior technicians and biomedical analysis engineers but only 6.6% of the laboratories were directed by biologists, and 9.2% of the laboratories function with only one technician. Instead of some assets, the laboratories assessment noted important non-conformities we ought to raise as a matter of urgency. It is a challenge whose resolution must give blood transfusion centers a reference position relatively to blood grouping when facing blood typing difficulties. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  12. Proportion of Rh phenotypes in voluntary blood donors.

    Science.gov (United States)

    Sarkar, R S; Philip, Joseph; Mallhi, R S; Yadav, Pramod

    2013-10-01

    The Rh system is the major blood group system besides ABO system. Even after proper blood grouping and cross matching there is a possibility of alloimmunization and antibody production in the recipients against the Rh or minor blood group antigens like Kell, MNSs, Duffy etc. Keeping in view the heavy financial burden of complete phenotyping of blood; the determination of only Rh phenotypes can play a major role in preventing alloimmunization and adverse events in multitransfusion cases. To determine the proportion of Rh phenotypes in voluntary blood donors with a view to generate blood bank data for constitution of panel of blood donors for multipurpose utilities. Identification of Rhesus factors (Rh) was done by the antigen antibody agglutination test by the test tube method on 10,133 healthy voluntary donors. The phenotypic frequencies of Rh blood groups in the studied population were D-92.25%, C-87.55%, E-26.55%, c-51.06% and e-98.42%. Thus 'e' was the most common and E was the least common of all the Rh types. Phenotypically DCCee group was the most common phenotype and dccee was least common type. Determination of Rh phenotypes can play a major role in preventing alloimmunization and avoiding adverse events in multitransfusion cases.

  13. Evaluation of an automated microplate technique in the Galileo system for ABO and Rh(D) blood grouping.

    Science.gov (United States)

    Xu, Weiyi; Wan, Feng; Lou, Yufeng; Jin, Jiali; Mao, Weilin

    2014-01-01

    A number of automated devices for pretransfusion testing have recently become available. This study evaluated the Immucor Galileo System, a fully automated device based on the microplate hemagglutination technique for ABO/Rh (D) determinations. Routine ABO/Rh typing tests were performed on 13,045 samples using the Immucor automated instruments. Manual tube method was used to resolve ABO forward and reverse grouping discrepancies. D-negative test results were investigated and confirmed manually by the indirect antiglobulin test (IAT). The system rejected 70 tests for sample inadequacy. 87 samples were read as "No-type-determined" due to forward and reverse grouping discrepancies. 25 tests gave these results because of sample hemolysis. After further tests, we found 34 tests were caused by weakened RBC antibodies, 5 tests were attributable to weak A and/or B antigens, 4 tests were due to mixed-field reactions, and 8 tests had high titer cold agglutinin with blood qualifications which react only at temperatures below 34 degrees C. In the remaining 11 cases, irregular RBC antibodies were identified in 9 samples (seven anti-M and two anti-P) and two subgroups were identified in 2 samples (one A1 and one A2) by a reference laboratory. As for D typing, 2 weak D+ samples missed by automated systems gave negative results, but weak-positive reactions were observed in the IAT. The Immucor Galileo System is reliable and suited for ABO and D blood groups, some reasons may cause a discrepancy in ABO/D typing using a fully automated system. It is suggested that standardization of sample collection may improve the performance of the fully automated system.

  14. Profile of Rh, Kell, Duffy, Kidd, and Diego blood group systems among blood donors in the Southwest region of the Paraná state, Southern Brazil.

    Science.gov (United States)

    Zacarias, Joana Maira Valentini; Langer, Ieda Bernadete Volkweis; Visentainer, Jeane Eliete Laguila; Sell, Ana Maria

    2016-12-01

    The aim of this study was to assess the distribution of alleles and genotypes of the blood group systems Rh, Kell, Duffy, Kidd, and Diego in 251 regular blood donors registered in the hemotherapy unit of the Southwestern region of Paraná, Southern Brazil. The frequencies were obtained by direct counting on a spreadsheet program and statistical analyses were conducted in order to compare them with other Brazilian populations using chi-squared with Yates correction on OpenEpi software. The frequencies of RHD* negative, RHCE*c/c and RHCE*e/e were higher than expected for the Caucasian population. A difference was also observed for FY alleles, FY*01/FY*01 genotype and FY*02N.01 -67T/C (GATA Box mutation). Two homozygous individuals were defined as a low frequency phenotype K + k- (KEL*01.01/KEL*01.01) and, for Diego blood group system the rare DI*01 allele was found in ten blood donors, of which one was DI*01/DI* 01 (0.4%). The allele and genotype frequencies of Kidd blood group system were similar to expected to Caucasians. The results showed the direction in which to choose donors, the importance of extended genotyping in adequate blood screening and the existence of rare genotypes in Brazilian regular blood donors. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran in blood donors of south Gujarat, India

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    Manoj A Kahar

    2014-01-01

    Full Text Available Background: This is the first study on phenotype frequencies of various blood group systems in blood donors of south Gujarat, India using conventional tube technique. Material and Methods: A total of 115 "O" blood group donors from three different blood banks of south Gujarat were typed for D, C, c, E, e, K, Jk a , Le a , Le b , P 1 , M, and N antigens using monoclonal antisera and k, Kp a , Kp b , Fy a ,Fy b , Jk b , S,s, Lu a , and Lu b antigens were typed using polyclonal antisera employing Indirect Antiglobulin Test. Antigens and phenotype frequencies were expressed as percentages. Results: From the 115 blood donor samples used for extended antigen typing in the Rh system, e antigen was found in 100% donors, followed by D [84.35%], C [81.74%], c [56.32%], and E [21.74%] with DCe/DCe (R 1 R 1 , 40.87% as the most common phenotype. k was found to be positive in 100% of donors and no K+k- phenotype was found in Kell system. For Kidd and Duffy blood group system, Jk(a+b+ and Fy(a-b- were the most common phenotypes with frequency of 52.17% and 48.69%, respectively. In the MNS system, 39.13% donors were typed as M+N+, 37.39% as M+N-, and 23.48% as M-N+. S+s+ was found in 24.35% of donors, S+s- in 8.69%, and S-s+ as the commonest amongst donors with 66.96%. No Lu(a+b+ or Lu(a+b- phenotypes were detected in 115 donors typed for Lutheran antigens. A rare Lu(a-b- phenotype was found in 2.61% donors. Conclusion: Data base for antigen frequency of various blood group systems in local donors help provide antigen negative compatible blood units to patients with multiple antibodies in order to formulate in-house red cells for antibody detection and identification and for preparing donor registry for rare blood groups.

  16. A study on weak D and the function of the Rh complex in red blood cells

    NARCIS (Netherlands)

    M.B. Hemker (Mirte)

    2004-01-01

    textabstractRhesus (Rh) is the most complex of the blood group systems. In this general introduction several aspects of Rh are described. The immunogenicity is responsible for its discovery and establishes its clinical importance. Subsequently the following characteristics of the Rh complex are

  17. Isolation, Cloning, Expression and Purification of Recombinant RhD Antigen from Cord Blood

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    M Habibi Roudkenar

    2008-09-01

    Full Text Available "nBackground: Rh (Rhesus is a highly complex blood group system in man deeply rooted in transfusion medicine. Isolation of RhD from cord blod, cloning and expression of recombinant RhD antigen in bacterial expression system was the aim of this study."nMethods: Total RNAs were extracted from cord blood (O+.  The quality of RNA was determined by electrophoresis. In or­der to obtain coding sequence of RhD antigen cDNA was synthesized and Rh D gene was amplified by RT-PCR. The iso­lated RhD gene was   cloned to pUC18 vector and transformed to DH5α. The confirmed construct was sub cloned into expres­sion vector, pBADgIII/A, and expressed in Top10 E.coli. The expressed protein was characterized by SDS-PAGE and western blot analysis. Antigenicity of the expressed protein was assessed by ELISA using commercially available hu­man anti-RhD polyclonal   antibody with   peroxidase conjugated goat anti-human IgG, IgM, IgA as secondary antibody. "nRe­sults: RhD gene was successfully cloned and expressed. The expected size of recombinant RhD protein was detected in SDS-PAGE, and confirmed by dot and western blot analysis. RhD antibody reacted with recombinant RhD antigen as well as with RhD polypeptide extracted from RBCs membrane."nConclusion: The recombinant RhD may be helpful to further investigate the molecular basis of RhD protein and could be applica­ble for production anti- D antibody in an animal model.

  18. ABO/RH DISTRIBUTION PATTERN AND PREVALENCE OF IRREGULAR ANTIBODIES IN HEALTHY BLOOD DONORS OF UTTARAKHAND

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    Brijesh Thakur

    2016-07-01

    Full Text Available BACKGROUND Blood group plays a vital role in transfusion safety, understanding genetics, inheritance pattern and disease susceptibility. This study is aimed to determine distribution pattern of ABO and Rh blood group, incidence and identification of irregular antibodies among blood donors of Uttarakhand. METHOD ABO/Rh blood grouping was performed by test tube agglutination method (both cell and serum grouping using antisera A, B and Rh from Tulip and Orthodiagnostics. Donors’ plasma were screened for irregular antibodies by indirect antiglobulin technique using Bio-Rad ID-Card “LISS/Coombs” and test cell reagents “ID-DiaCell” via gel centrifugation. RESULTS Out of total 4573 included blood donors, 88.2% were males. The maximum blood donors (77.6% were in 18-25 years of age group. The commonest ABO blood group present was B (36.1% followed by O (29.1%, A (24.8% and AB (10% in blood donors; while in Rhesus system, 95.0% donors were Rh-positive and 5.0% donors were Rh negative. Screening of donors’ plasma did not reveal any detectable irregular antibody. CONCLUSION The study has a significant implication in suggesting the distribution pattern of ABO/Rh blood group typing in the region, but routine irregular antibody screening in healthy blood donors may not be fruitful in this region keeping cost effectiveness and absence of previous sensitization in mind

  19. Impact of a confirmatory RhD test on the correct serologic typing of blood donors

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    Luciana Cayres Schmidt

    2015-10-01

    Full Text Available BACKGROUND: The RHD gene is highly polymorphic, which results in a large number of RhD variant phenotypes. Discrepancies in RhD typing are still a problem in blood banks and increase the risk of alloimmunization. In this study, the RhD typing strategy at a blood bank in Brazil was evaluated.METHODS: One-hundred and fifty-two samples typed as RhD negative and C or E positive by routine tests (automated system and indirect antiglobulin test using the tube technique were reevaluated for RhD status by three methods. The method with the best performance was implemented and evaluated for a period of one year (n = 4897 samples. Samples that were D positive exclusively in the confirmatory test were submitted to molecular analysis.RESULTS: The gel test for indirect antiglobulin testing with anti-D immunoglobulin G (clone ESD1 presented the best results. Seventy samples (1.43% previously typed as RhD negative showed reactivity in the gel test for indirect antiglobulin testing and were reclassified as D positive. D variants that may cause alloimmunization, such as weak D type 2 and partial DVI, were detected.CONCLUSION: The confirmatory RhD test using the gel test for indirect antiglobulin testing represents a breakthrough in transfusion safety in this blood center. Our results emphasize the importance of assessing the blood group typing strategy in blood banks.

  20. ABO and Rh Blood Groups Distribution in Yozgat City, Turkey

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    Çiğdem Kader

    2014-06-01

    Full Text Available Objective: In this retrospective study, we included 5257 healthy participants who admitted to Yozgat Government Hospital Blood Bank and Bozok University Blood Bank for several reasons to determine A, B, 0, AB blood groups and Rhesus (Rh positivity ratios between January 2007 and April 2013. Methods: We recorded their blood group types, age and gender from the hospital data. 2430 females, 2827 males totally 5257 participants were included to our study. Results: 2330 (44.3% persons were A blood group, 837 (15.9% were B group, 1665 (31.7% were 0 and 425 (%8.1 were AB group. Rh positivity ratio of our study group was 88%. Blood group frequency of Yozgat city is similar to other cities and the general population of our country, Turkey. Conclusion: Knowing the blood group is important for the blood recruitment in our region. J Clin Exp Invest 2014; 5 (2: 169-172

  1. FREQUENCY AND DISTRIBUTION OF ABO & RH BLOOD GROUP IN BILASPUR DISTRICT OF CHHATTISGARH STATE : A STUDY FROM MEDICAL COLLEGE HOSPITAL

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    Bhanu Pratap

    2015-07-01

    Full Text Available BACKGROUND : Approximate 30 blood group systems have discovered and more than 400 erythrocytes antigens are identified. Blood group ABO and Rh are most important among all other blood group systems in transfusion service practices. The frequency of four major blood gr oup s namely A, B, O, AB with Rh Positive and Negative varies in different population of the world and differ also in region and race wise. MATERIAL AND METHOD : This 5 years retrospective study was conducted at Blood Bank of a Medical college Hospital of Bi laspur in Northern Chhattisgarh, catering the 1/3 population of state. Data were collected from the Blood Bank Grouping record from the period of January 2010 to December 2014. Blood group of blood donors and patients were determined by Monoclonal Anti Ser a by slide agglutinations tests. Rare case and difficult case were examined by test tube agglutination method and Matrix Gel System of Tulip. RESULT AND CONCLUSIO N: 31973 subjects were examined for blood group during observation period, Out of these 31092( 97.25% were male and 881 (2.75% were female. The frequency of blood group B in these populations was 11007 (34.42% (33.36% Rh Positive and 1.06% Rh Negative Followed by O were 10864 (33.97% (33.33% Rh Positive and 0.64% Rh Negative, A was 9113 (28.50 % (27.99 % Rh Positive and 0.51% Rh Negative and AB was 989 (3.11% (3.01% Rh Positive and 0.1% Rh Negative. Rhesus group Rh Positive were 31242 (97.7 % and Rh Negative were 731 (2.3 %.

  2. ABO and Rh-D blood group frequency and distribution: a tertiary care hospital experience

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    Pandu Rangarao Sanagapati

    2015-08-01

    Results: Out of 1740 blood donors, 1702 (55.6% were male and 38 (44.4% were female. Majority of blood donors were in 21-40 years of age group. The most frequent blood group positions in the descending order are and lsquo;O', and lsquo;A', and lsquo;B' and and lsquo;AB' respectively. One group was and lsquo;Oh' (Bombay Phenotype. Conclusions: and lsquo;O' group is the most frequent position of ABO blood group system followed by and lsquo;A' group. Rh+ is the most frequent group than Rh- in the Rh system. Blood donations by females are very low. [Int J Res Med Sci 2015; 3(8.000: 2058-2061

  3. Rh incompatibility

    Science.gov (United States)

    ... that develops when a pregnant woman has Rh-negative blood and the baby in her womb has Rh- ... against Rh-positive blood. However, women with Rh-negative blood type must get injections: During every pregnancy After ...

  4. Rh phenotype, allele and haplotype frequencies among 51,857 blood donors in North India.

    Science.gov (United States)

    Makroo, Raj; Gupta, Richa; Bhatia, Aakanksha; Rosamma, Nakamatathil L

    2014-01-01

    This study was performed to provide information on the frequencies of Rh antigens, alleles, phenotypes, and haplotypes from our region in India and to compare them with those from other races. This observational study was conducted on blood donors from March 2009 to August 2011 using a fully automated system for Rh typing of blood cells. The data were collected and calculations done to determine the antigen, phenotypes, allele and haplotype frequencies. The chi square test was used for comparisons between the results of our study and those of other studies. A total of 51,857 donors were included in this study. The most common Rh antigen found was "e". DCCee was the most prevalent phenotype in our study with the phenotype distribution being significantly different between our study and other studies from different regions of the world. We have determined the prevalence of Rh antigens and Rh phenotypes in the North Indian blood donor population and derived the allele and haplotype frequencies in the same population. The Rh blood group distribution in this population was different from that in other populations.

  5. Rh Disease

    Science.gov (United States)

    ... Loss > Birth defects & other health conditions > Rh disease Rh disease E-mail to a friend Please fill ... Rh-negative with a blood test. What is Rh factor? Rh factor is a protein that’s found ...

  6. Prevalence of Principal Rh Blood Group Antigens in Blood Donors at the Blood Bank of a Tertiary Care Hospital in Southern India.

    Science.gov (United States)

    Gundrajukuppam, Deepthi Krishna; Vijaya, Sreedhar Babu Kinnera; Rajendran, Arun; Sarella, Jothibai Dorairaj

    2016-05-01

    Rhesus (Rh) antigen was discovered in 1940 by Karl Landsteiner and Wiener. Due to its immunogenicity along with A, B antigens, Rh D antigen testing was made mandatory in pre-transfusion testing. Presently there are more than 50 antigens in Rh blood group system but major ones are D, C, E, c, and e. Very few reports are available regarding their prevalence in India and no reports are available from Andhra Pradesh. To study the prevalence of principal Rh blood group antigens like D, C, E, c & e in the voluntary blood donors attending our blood bank. A prospective cross-sectional non interventional study was carried out on 1000 healthy blood donors from August 2013 to July 2014 at our blood bank. Donors were grouped and typed for ABO and Rh major antigens using monoclonal blood grouping reagents as per the manufacturer's instructions. Statistical analysis was carried out using SPSS version 16. Comparison of categorical data between antigen positive and negative individuals was done using Chi-square test. Descriptive statistics for the categorical variables were performed by computing the frequencies (percentages) in each category. Incidence was given in proportion with 95% confidence interval. A total of 1000 blood samples from donors were phenotyped. Among Rh antigens, e was the most common antigen (98.4%), followed by D-94.1%, C-88%, c-54.9% and E-18.8% with DCe/DCe (R1R1) (43.4%) being the most common phenotype and the least common phenotype is r'r' (0.1%). Database for antigen frequency to at least Rh blood group system in local donors helps to provide antigen negative blood to patients with multiple alloantibodies, minimize alloimmunization rate, and thereby improve blood safety.

  7. High rhesus (Rh(D)) negative frequency and ethnic-group based ABO blood group distribution in Ethiopia.

    Science.gov (United States)

    Golassa, Lemu; Tsegaye, Arega; Erko, Berhanu; Mamo, Hassen

    2017-07-26

    Knowledge of the distribution of ABO-Rh(D) blood groups in a locality is vital for safe blood services. However, the distribution of these blood systems among Ethiopians in general is little explored. This study was, therefore, designed to determine the ABO-Rh(D) blood group distribution among patients attending Gambella hospital, southwestern Ethiopia. A cross-sectional study was conducted between November and December 2013 (N = 449). The patients were grouped into two broad categories. Those who originally moved from different parts of Ethiopia and currently residing in Gambella are named 'highlanders' (n = 211). The other group consisted of natives (Nilotics) to the locality (n = 238). ABO-Rh(D) blood groups were typed by agglutination, open-slide test method, using commercial antisera (Biotech laboratories Ltd, Ipswich, Suffolk, UK). Overall, majority of the participants (41.20%) had blood type 'O' followed by types 'A' (34.96%), 'B' (20.48%) and 'AB' (3.34%). However, blood type 'A' was the most frequent (44.07%) blood group among the 'highlanders' and 50.42% of Nilotic natives had type 'O'. The proportion of participants devoid of the Rh factor was 19.37%. While the ABO blood group distribution is similar to previous reports, the Rh(D) frequency is much higher than what was reported so far for Ethiopia and continental Africa.

  8. ABO/Rh Blood-Typing Model: A Problem-Solving Activity

    Science.gov (United States)

    Wake, Carol

    2005-01-01

    An ARO/Rh Blood-Typing kit useful for students to visualize blood-typing activities and practice problem-solving skills with transfusion reactions is presented. The model also enables students to identify relationships between A, B, and Rh antigens and antibodies in blood and to understand molecular mechanisms involved in transfusion agglutination…

  9. ABO/Rh Blood-Typing Model: A Problem-Solving Activity

    Science.gov (United States)

    Wake, Carol

    2005-01-01

    An ARO/Rh Blood-Typing kit useful for students to visualize blood-typing activities and practice problem-solving skills with transfusion reactions is presented. The model also enables students to identify relationships between A, B, and Rh antigens and antibodies in blood and to understand molecular mechanisms involved in transfusion agglutination…

  10. Optimal $RH_2$-- and $RH_\\infty$--Approximation of Unstable Descriptor Systems

    CERN Document Server

    Köhler, Marcus

    2012-01-01

    We consider the following problem. Given an unstable continuous--time descriptor system with transfer function $G$, find a stable descriptor system whose transfer function is the best approximation of $G$ in the spaces $RH_2$ and $RH_\\infty$, respectively. Explicit optimal solutions are presented under consideration of numerical issues.

  11. Genetic Peculiarities of Blood Group Distribution in Infants Born to Mothers With 0(I Rh(+ Blood Group in Bukovyna Region

    Directory of Open Access Journals (Sweden)

    Оksana G. Cherniukh

    2015-06-01

    Full Text Available The article presents the analysis of distribution of the group (according to AB0 system and rhesus characteristics (according to anti-D system in infants born to mothers with 0(I Rh(+ blood group in Chernivtsi region (Ukraine during the period of 2013-2014. The relationship of the umbilical bilirubin level and hemolytic disease of the newborn (HDN (erythroblastosis fetalis, the necessity of its careful monitoring during the first day of life of a newborn, especially in case a baby belongs to a certain risk group, has been analyzed. The issue concerning probable HDN occurrence from mothers with А(II Rh(+ blood group which is dominant in this region has been touched upon. The tasks for further work in this direction of ecopathophysiology in the areas of gene penetration have been assigned.

  12. ABO and RhD blood group typing by microplate automatic detection system%大批量微板自动化检测正反定ABO及RhD血型的研究

    Institute of Scientific and Technical Information of China (English)

    赵君; 邢培清; 刘玉振; 方建华; 马宏伟; 温涛; 吕永磊

    2007-01-01

    目的:探讨用微孔板代替纸板实现仪器自动化检测正反定ABO以及RhD血型.方法:利用加样器取自试管内的红细胞或血浆,然后分配到微板内,经离心、振荡,最后在微板观察仪上进行判断,在局域网上发送报告.结果:正定ABO及RhD仪器优化参数为:在预稀释板上加生理盐水147μl,吸标本压积红细胞3μl,使红细胞浓度达到2%;吸红细胞的液体参数前吐后弃体积均为0μl,并且吸前不混合;在预稀释板上分配,加样针离微板的距离为5 mm;2%红细胞与抗-A或抗-B体积选择均为25μl;预稀释板上剩余的50μl混合红细胞作为RhD检测用,抗-D体积为25μl.反定ABO优化参数为:红细胞浓度选择3%,体积为25μl,血浆50 μl.离心转速为1000 r/min,时间1 min;振荡15 s,静止2 min,观察结果.本方法B3、A3弱亚型均能检出,和纸板法符合率为100%,500份样本正反定ABO以及RhD血型,从准备到发结果仅需2 h.结论:本研究实现了正反定ABO及RhD血型的自动化操作,不但省时省工,而且结果准确可靠,减少了微板和抗-D、抗-A和抗-B的用量,具有显著的经济效益和社会效益.

  13. VEGF signalling controls GnRH neuron survival via NRP1 independently of KDR and blood vessels.

    Science.gov (United States)

    Cariboni, Anna; Davidson, Kathryn; Dozio, Elena; Memi, Fani; Schwarz, Quenten; Stossi, Fabio; Parnavelas, John G; Ruhrberg, Christiana

    2011-09-01

    Gonadotropin-releasing hormone (GnRH) neurons are neuroendocrine cells that are born in the nasal placode during embryonic development and migrate through the nose and forebrain to the hypothalamus, where they regulate reproduction. Many molecular pathways that guide their migration have been identified, but little is known about the factors that control the survival of the migrating GnRH neurons as they negotiate different environments. We previously reported that the class 3 semaphorin SEMA3A signals through its neuropilin receptors, NRP1 and NRP2, to organise the axons that guide migrating GnRH neurons from their birthplace into the brain. By combining analysis of genetically altered mice with in vitro models, we show here that the alternative neuropilin ligand VEGF164 promotes the survival of migrating GnRH neurons by co-activating the ERK and AKT signalling pathways through NRP1. We also demonstrate that survival signalling relies on neuronal, but not endothelial, NRP1 expression and that it occurs independently of KDR, the main VEGF receptor in blood vessels. Therefore, VEGF164 provides survival signals directly to developing GnRH neurons, independently of its role in blood vessels. Finally, we show that the VEGF164-mediated neuronal survival and SEMA3A-mediated axon guidance cooperate to ensure that migrating GnRH neurons reach the brain. Thus, the loss of both neuropilin ligands leads to an almost complete failure to establish the GnRH neuron system.

  14. Advance of Rh blood group system and its clinical application%人类Rh血型研究进展与临床应用

    Institute of Scientific and Technical Information of China (English)

    申卫东; 唐秋民

    2009-01-01

    Rh血型是继ABO血型发现后的临床意义最大的一个血型,也是最复杂、最富有多态性的红细胞血型系统.现代分子生物学研究证明,传统的Rh抗原是由RHD和RHCE两个基因编码,其中RHD基因编码RhD多肽,RHCE基因编码RhC/c和RhE/e多肽.Rh血型系统抗原抗体的不匹配能引起溶血性输血反应、新生儿溶血病和自身免疫性溶血性贫血.

  15. ABO and Rh Blood Type Relationship in Parents with more than One Disabled Child

    Science.gov (United States)

    Mehrmohammadi, M

    2015-01-01

    Background Parental blood variables are one of the most important medical-biological causes of intellectual and physical-movement disabilities. The purpose of this study was to identify the relationship between parents’ blood variables (ABO and Rh blood type) and their relationship with frequency of intellectual and physical-movement disabilities in Isfahan province. Materials and Methods This was a descriptive-analytical study and 494 samples were selected from mothers with more than one disabled child and mothers with normal child using simple and multistage random methods. The data collection was done through questionnaire. Based on Kuder-Richardson Formula 20 (KR-20), the reliability of questionnaire was 0.88. The statistical model in this study was a hierarchical log-linear method. Results There was a significant relationship between mother’s Rh blood and having disabled child (P=0.002). However no significant relationship between having disabled children and the following variables was found: the father’s Rh blood (p=0.2), father and mother’s Rh blood together (P=0.5), father blood type (P=0.56), mother blood type (P=0.42), and mother and father blood types together (P=0.7). Conclusion Maternal and fetal blood incompatibility (motherwith negative Rh blood and fetus with positive Rh blood) increased the likelihood of being born with disabilities. PMID:26985351

  16. Distribution of ABO and Rh blood groups in patients with HELLP syndrome.

    Science.gov (United States)

    Sezik, M; Toyran, H; Yapar, E G

    2002-11-01

    The aim of this retrospective study was to evaluate the relationship between HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome and the maternal blood groups. Five hundred and forty-seven women with severe preeclampsia were included and divided into eight groups according to their blood groups: A Rh-positive (n=203), A Rh-negative (n=38), B Rh-positive (n=83), B Rh-negative (n=10), 0 Rh-positive (n=148), 0 Rh-negative (n=21), AB Rh-positive (n=39), and AB Rh-negative (n=5). The groups were controlled by analysis of variance and found to be homogeneous with respect to parity, gestational age, blood pressure, hemoglobin, hematocrit, platelet values, prothrombin time, partial thromboplastin time, fibrinogen, creatinine, alanine aminotransferase, aspartate aminotransferase, bilirubin, uric acid, and proteinuria. Incidence of HELLP syndrome was 24% in the overall study population whereas 48% of the patients with the blood group O Rh-negative had HELLP syndrome associated with an increase in risk by a factor of 3.1. To our knowledge this is the first report of such an association.

  17. Distribution of ABO and Rh types in voluntary Blood donors in Jharkhand area as a study conducted by RIMS, Ranchi.

    Science.gov (United States)

    Singh, Anu; Srivastava, Ramesh Kumar; Deogharia, Kabita S; Singh, Kranti Kumar

    2016-01-01

    This study was done to know the distribution and frequencies of blood groups among blood donors attending voluntary blood donation camps organized by the Rajendra Institute of Medical Sciences (RIMS), Ranchi, Jharkhand so that demand and supply ratio of the four blood groups can be maintained so that no patient dies due to lack of a particular blood group. Up till now about 400 red cells antigen have been identified. The majority follow Mendelian inheritance. The ABO and Rhesus (Rh) blood group system are most important for blood transfusion purposes, parental testing, legal medicine, and in population genetic study. This study was conducted to determine and compare the frequency and distribution of ABO and Rh blood groups among voluntary blood donors attending blood donation camps in Jharkhand organized by RIMS. The aim is to know the demand and supply ratio of a particular blood group in light of their distribution in the society so that no patient dies due to the deficient supply of blood. It is a retrospective study carried out at blood bank. Materials and Methods: A study was conducted at Blood bank, RIMS, Ranchi, Jharkhand, over a period of 4 years from January 1, 2012, to December 31, 2015. Blood group of the blood donors was determined by commercially available standard monoclonal antisera by test tube agglutination technique accompanied by reverse grouping. Out of 20,455 subjects, 18,717 (91.73%) were male and 1738 (8.27%) were female subjects. The ABO blood group present was B (35.15%) followed by O (34.73%), A (22.09%), and AB (8.03%) in blood donors while in Rh system, (96.46%) donors were Rh +ve and (3.54%) donors were Rh -ve. The study has a significant implication regarding the inventory management of blood bank and transfusion services for the indoor patients of RIMS and for emergency supply to other hospitals of Jharkhand in dire need of blood. The knowledge of distribution of blood group is very important for blood banks and transfusion services

  18. Distribution of ABO and Rh Blood Groups in Patients With Keratoconus: A Case-Control Study.

    Science.gov (United States)

    Naderan, Mohammad; Rajabi, Mohammad Taher; Shoar, Saeed; Kamaleddin, Mohammad Amin; Naderan, Morteza; Rezagholizadeh, Farzaneh; Zolfaghari, Masoome; Pahlevani, Rozhin

    2015-07-01

    Association of keratoconus (KC) with genetic predisposition and environmental factors has been well documented. However, no single study has investigated the possible relationship between ABO and Rh blood groups and KC. A case-control study was designed in a university hospital enrolling 214 patients with KC in the case group and equal number of age- and sex-matched healthy subjects in the control group. Primary characteristics, ABO blood group, and Rh factors were compared between the two groups. Topographic findings of KC eyes and the severity of the diseases were investigated according to the distribution of the blood groups. Blood group O and Rh(+) phenotype were most frequent in both groups. There was no significant difference between the two groups in terms of ABO blood groups or Rh factors. Mean keratometery (K), central corneal thickness, thinnest corneal thickness, flat K, steep K, sphere and cylinder, spherical equivalent, and uncorrected visual acuity were all similar between ABO blood groups and Rh(+) and Rh(-) groups. However, the best spectacle-corrected visual acuity (BCVA) had the highest value in AB blood group (0.35 ± 0.22 logMAR, P=0.005). Moreover, the blood group AB revealed the highest frequency for grade 3 KC, followed by grades 1, 2, and 4 (P=0.003). We observed no significant excess of any particular blood group among KC cases compared with healthy subjects. Except BCVA, none of the keratometric or topographic findings was significantly different between blood groups.

  19. Gene Frequency and Heritability of Rh Blood Group Gene in 44 Human Populations

    Directory of Open Access Journals (Sweden)

    Supriyo CHAKRABORTY

    2010-09-01

    Full Text Available The frequency of RhD and Rhd alleles of Rh blood group gene was estimated in 44 human populations distributed all over the world from the RhD phenotypic data. The average frequency of RhD and Rhd allele over these populations was 0.70 and 0.30, respectively. Higher frequency of RhD allele than the expected estimate (0.50 in all the populations, under Hardy-Weinberg equilibrium condition assuming equal frequency of both alleles in the initial population, indicated inbreeding at RhD/d locus as well as natural selection for RhD allele. Very high heritability estimate (84.04% of Rh allele frequency revealed that this trait was under weak selection pressure and resulted in greater genetic variation in existing populations. It is consistent with Fishers fundamental theorem of natural selection. The results from the present study suggest that inbreeding at RhD/d locus and some other factors (possibly mutation, migration and genetic drift other than natural selection alone played major roles in changing the Rh allele frequency in these populations.

  20. Is current serologic RhD typing of blood donors sufficient for avoiding immunization of recipients?

    DEFF Research Database (Denmark)

    Krog, Grethe Risum; Clausen, Frederik Banch; Berkowicz, Adela

    2011-01-01

    Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary.......Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary....

  1. Prevalence of RhD variants among blood donors at Gulu Regional Blood Bank, Gulu, Northern Uganda

    Directory of Open Access Journals (Sweden)

    Ojok P

    2017-09-01

    Full Text Available Polycarp Ojok,1,2 Caesar Oyet,1 Fred Webbo,1,3 Bashir Mwambi,1 Ivan M Taremwa1 1Institute of Allied Health Sciences, International Health Sciences University, Kampala, 2Gulu Regional Blood Bank, Gulu, 3Lancet Laboratories, Kampala, Uganda Aim/objective: The aim of this study was to determine the prevalence of RhD variant ­phenotypes among voluntary non-remunerated blood donors (VNRBDs at Gulu Regional Blood Bank (GRBB, Northern Uganda. Materials and methods: We conducted a cross-sectional study, in which the first 4.0 mL of ethylenediaminetetraacetic acid (EDTA blood samples were collected from VNRBDs and typed for their ABO and RhD blood group status using IgM and IgG monoclonal typing antisera, respectively. Blood samples that tested as RhD negative were further investigated for RhD variant phenotypes using indirect antihuman globulin hemagglutination technique. Results: We assayed 138 RhD-negative blood samples obtained from VNRBDs. Of these, 66.7% (n=92 were males. Their median age was 24.4 years (range, 14–33 years. Majority of the participants were of ABO blood group O (62.8%, n=86, followed by A (19.7%, n=27, then B (13.9%, n=19 and least AB (3.6%, n=6. The prevalence of RhD variant phenotypes was 0.7% (n=1; 95% confidence interval, 0.5–0.9. There was no statistical association of RhD variant phenotypes with donor gender, tribe and their ABO blood groups. Conclusion: This study has revealed a high prevalence of RhD variant among blood donors at GRBB in Northern Uganda. It further highlights a potential risk of alloimmunization, as the present blood typing practices do not identify RhD variant phenotypes. Keywords: Rh blood group, D variants, D antigen, weak D, partial D, Uganda 

  2. Investigation on 43 cases of patients in Rh (D) -negative blood group%43例住院病人Rh(D)阴性血型调查

    Institute of Scientific and Technical Information of China (English)

    黄秀琼; 陈文珠

    2012-01-01

    Objective To understand the distribution and frequency of Rho ( D ) -negative blood group phenotype of patients in Foshan Hospital of Traditional Chinese Medicine, Establish the blood group archives of Rh ( D )-negative patients and blood donors.Methods screening Rh( D ) Antigen by Johnson AutoVue Innova matching and automatic blood Analyzer, confirming Rh ( D ) -negative Antigen by indirect antihuman globulin test.And then filtering and classifing With the Rh factor c,C,E and e using test tube method.Results 43 cases of Rh ( D ) negative patients were tested In 16402,including type A of 11,type B of 11,type O of 19,type AB of 3; Rh phenotype were Ccdee 11,ccdee 30,CCdee 2.Conclusions the Rh( D ) negative rate was 0.26%,Conforms to China's Han population of Rh ( D ) -negative rates.It has a very important role in Mastering the Rh ( D ) -negative blood types distribution and frequency on Foshan crowd,establishing blood types archives of Rh( D )-negative,using emergency blood of rare blood type and reducing transfusion reaction of patients with immune.%目的 调查佛山市中医院住院病人Rh (D)阴性血型,了解Rh (D)阴性血型的表现型分布和频率,建立起Rh(D)阴性患者和献血者的血型档案,保证临床安全输血.方法 采用强生AutoVue Innova 全自动血型与配血分析仪对Rh(D)抗原进行初筛检测;初筛阴性者再采用间接抗人球蛋白试验进行确认;确认阴性者用试管法对Rh因子C、c、E、e进行筛选和分型.结果 在16 402人中检出Rh(D)阴性血员43人,其中A型11人,B型11人,O型18人,AB型3人;43人的Rh表现型为:Ccdee 11人,ccdee 30人,CCdee2人.结论 佛山市中医院住院病人Rh(D)阴性率为0.26%,符合我国汉族人口Rh(D)阴性率.掌握佛山市人群Rh(D)阴性血型的分布和频率,建立Rh (D)阴性血型档案,对稀有血型患者的紧急用血及降低免疫性输血反应有非常重要的作用.

  3. [Frequencies of blood groups, ABO and Rh D incompatibility in post-delivery women and their liveborn].

    Science.gov (United States)

    Baiochi, Eduardo; Camano, Luiz; Sass, Nelson; Colas, Osmar Ribeiro

    2007-01-01

    This study aimed to assess the frequency of different blood phenotypes and to predict the risk of Rh D alloimmunization and maternal-fetal incompatibility in a Brazilian population living in the West zone of the city of São Paulo-Brazil. This descriptive study evaluated 2,372 post-delivery women and their liveborn during one year. Blood types were analyzed by means of tube agglutination tests. The blood type frequencies were: 50.67 O, 32.17 A, 13.45 B, 3.75 AB, 90.34 Rh D(+) and 9.66 Rh D(-). ABO maternal-fetal incompatibility was detected in 18.4% and Rh D incompatibility in 7%. The fraction of Rh D(-) population at high risk for Rh D alloimmunization was 82%, emphasizing the importance of Rh D alloimmunization profilaxis.

  4. Living with Rh Incompatibility

    Science.gov (United States)

    ... Living With Rh Incompatibility If you have Rh-negative blood, injections of Rh immune globulin can reduce your ... baby or come in contact with Rh-positive blood. If you're Rh-negative, your risk of problems from Rh incompatibility is ...

  5. No Distinction of Orthology/Paralogy between Human and Chimpanzee Rh Blood Group Genes.

    Science.gov (United States)

    Kitano, Takashi; Kim, Choong-Gon; Blancher, Antoine; Saitou, Naruya

    2016-02-12

    On human (Homo sapiens) chromosome 1, there is a tandem duplication encompassing Rh blood group genes (Hosa_RHD and Hosa_RHCE). This duplication occurred in the common ancestor of humans, chimpanzees (Pan troglodytes), and gorillas, after splitting from their common ancestor with orangutans. Although several studies have been conducted on ape Rh blood group genes, the clear genome structures of the gene clusters remain unknown. Here, we determined the genome structure of the gene cluster of chimpanzee Rh genes by sequencing five BAC (Bacterial Artificial Chromosome) clones derived from chimpanzees. We characterized three complete loci (Patr_RHα, Patr_RHβ, and Patr_RHγ). In the Patr_RHβ locus, a short version of the gene, which lacked the middle part containing exons 4-8, was observed. The Patr_RHα and Patr_RHβ genes were located on the locations corresponding to Hosa_RHD and Hosa_RHCE, respectively, and Patr_RHγ was in the immediate vicinity of Patr_RHβ. Sequence comparisons revealed high sequence similarity between Patr_RHβ and Hosa_RHCE, while the chimpanzee Rh gene closest to Hosa_RHD was not Patr_RHα but rather Patr_RHγ. The results suggest that rearrangements and gene conversions frequently occurred between these genes and that the classic orthology/paralogy dichotomy no longer holds between human and chimpanzee Rh blood group genes. © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  6. Prevalence and gene frequencies of A1A2BO and Rh(D) blood ...

    African Journals Online (AJOL)

    Ruqaiya Hussain

    2012-08-03

    Aug 3, 2012 ... India's strategic location as a migratory crossroad may allow us to ascertain the ... important because of its old centers of population, learning and administration. ... blood groups A1A2BO and Rh(D) distribution among some.

  7. Survey the population distribution of ABO and Rh blood group in Huainan city%安徽省淮南市人群ABO及Rh血型分布调查

    Institute of Scientific and Technical Information of China (English)

    王玮; 孙友岭; 夏伯阳

    2012-01-01

    Objective To explore the ABO blood group and Rh blood group system antigen distribution in Huainan area.Methods To investigate the Huainan area of 24 035 voluntary blood donors of the ABO and Rh blood group system phenotype and gene frequency distribution by the population genetics of the blood type.Results In the Huainan area population,the gene frequency of ABO blood type distribution was r > P > q,the distribution of phenotype was O > A > B > AB,the distribution of RhD(-)frequency was 0.36%,and this result was consistent with the RhD(-)frequency of 0.2%~0.5% of the Han population in the country.Conclusion ABO blood group and Rh blood group were two separate systems,control their distributed rule in the Huainan region was very important to the clinical use of blood,establishment of a repository of rare blood group of RhD(-),alleviation the lack of state hospital transfusion,raising the level of clinical treatment and to the reducing the incidence of immunological transfusion reactions.%目的 调查安徽省淮南市人群ABO血型及Rh血型系统抗原分布状况.方法 用血型群体遗传学研究方法,选择淮南市的无偿献血者24 035名,进行ABO、Rh血型系统的表现型及基因频率分布调查分析.结果 淮南市人群ABO血型基因频率为r>P>q,表现型分布特征为O>A>B>AB,RhD(-)频率为0.36%,与全国汉族人群RhD(-)频率0.2%~0.5%相吻合.结论 ABO、Rh血型为两个独立的血型系统,掌握其在淮南市人群中的分布规律,对临床科学用血及RhD(-)稀有血型档案库的建立,缓解医院输血缺乏状况,提高临床治疗水平,降低免疫性输血反应的发生非常重要.

  8. Rh阴性血型患者输血相关问题探讨%Discussion on the issues related to Rh-negative blood transfusion

    Institute of Scientific and Technical Information of China (English)

    韩艳秋

    2016-01-01

    Rh血型系统是重要的红细胞血型系统,目前临床输血治疗对于Rh阴性血液的需求仍处于紧张状态.实际临床工作中,通过典型案例分析总结针对Rh阴性血型患者输血的临床经验,建立规范化的血库管理模式,分析Rh阴性血型患者的输血类型,并且积极普及Rh阴性血型的相关知识,尽力减少临床输血过程中导致的输血反应等问题,从而达到安全、合理输血的目的.%Rh blood group system is very important for red blood cell blood group system,the supply of Rh-negative blood have been remained on edge in clinical blood transfusions.During clinical practice,we should learn experience of blood transfusion in patients with Rh-negative blood type from clinical typical cases,establish a standardized blood bank management model,analyze the types of blood transfusion of Rh-negative patients,and popularize knowledge of Rh-negative rare blood type,try our best to reduce the probability of transfusion reaction,in order to ensure the clinical blood supply,and the blood quality and safety.

  9. [Radiation protection effect of rhIL-12 on monkey hematopoietic system].

    Science.gov (United States)

    Xiong, Guo-Lin; Zhao, Yi; Xing, Shuang; Shen, Xing; Ning, Xue-Cheng; Lu, Shi-Xiang; Li, Jian; Guo, Ling-Ling; Hao, Rui; Chen, Ting-Chao; Miao, Jin-Lai; He, Ji-Chen; Luo, Qing-Liang

    2013-02-01

    This study was aimed to investigate the radioprotective effects of recombinant human interleukin-12 (rhIL-12) on monkey hematopoietic system, and to provide experimental evidence for future clinical prophylaxis and treatment for patients who suffered from acute radiation syndrome. In in vitro study, the effect of rhIL-12 in different concentrations (0, 1, 5, 25, 125 and 625 ng/ml) on colony forming capacity of human or monkey bone marrow-derived mononuclear cells was examined in methylcellulose H4434 medium. In in vivo study, the acute radiation syndrome model was established in 11 Rhesus monkeys which received lethal total body irradiation by 6 Gy (60)Co γ in single time irradiation. The irradiated monkeys were randomly divided into 3 subgroups: control group (n = 4) which received subcutaneous PBS injection, rhIL-12 single-dose group (n = 3) which received subcutaneous single injection of rhIL-12 (4 µg/kg) at 2 h after irradiation, and multiple-dose group (n = 4) which received subcutaneous injection of rhIL-12 (1 µg/kg per injection) at 2 h, day 3, 6 and 9 after irradiation respectively. Peripheral blood cells were counted before and after irradiation every other day. The survival status of animals were observed daily. In vitro test results showed that different concentrations of rhIL-12 obviously promoted human and healthy monkeys' bone marrow mononuclear cells to form various hematopoietic progenitor cell colonies, especial CFU-E and CFU-GM. All animals in control group died within 22 d after lethal total body irradiation, average survival time was (20.3 ± 1.2) d. Only one monkey in multiple-dose group died due to anemia on day 17. All monkeys in single-dose group survived. Compared with control group, rhIL-12-administrated monkeys' white blood cell count, hemoglobin level, platelet and reticulocyte counts showed faster recovery from high dose radiation. It is concluded that the rhIL-12 treatment can promote the bone marrow hematopoietic stem

  10. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia

    Directory of Open Access Journals (Sweden)

    Getaneh Alemu

    2016-01-01

    Full Text Available Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22±0.29 (median ± standard error of the mean. Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%, A (136, 32.7%, B (87, 20.9%, and AB (18, 4.3%. Most of them were Rh+ (386, 92.8%. The overall malaria prevalence was 4.1% (17/416. ABO blood group is significantly associated with malaria infection (P=0.022. High rate of parasitemia was seen in blood group O donors (6.899, P=0.003 compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia.

  11. Assessing ABO/Rh Blood Group Frequency and Association with Asymptomatic Malaria among Blood Donors Attending Arba Minch Blood Bank, South Ethiopia.

    Science.gov (United States)

    Alemu, Getaneh; Mama, Mohammedaman

    2016-01-01

    Background. Determination of the various ABO/Rh blood group distributions and their association with malaria infection has paramount importance in the context of transfusion medicine and malaria control. Methods. Facility based cross-sectional study was conducted from February to June, 2015, to assess ABO/Rh blood groups distribution and their association with asymptomatic malaria. A structured questionnaire was used to collect data. Blood grouping was done using monoclonal antibodies. Thin and thick blood films were examined for Plasmodium parasites. Data were analyzed using SPSS version 20.0. Results. A total of 416 blood donors participated with median age of 22 ± 0.29 (median ± standard error of the mean). Distribution of ABO phenotypes, in decreasing order, was O (175, 42.1%), A (136, 32.7%), B (87, 20.9%), and AB (18, 4.3%). Most of them were Rh+ (386, 92.8%). The overall malaria prevalence was 4.1% (17/416). ABO blood group is significantly associated with malaria infection (P = 0.022). High rate of parasitemia was seen in blood group O donors (6.899, P = 0.003) compared to those with other ABO blood groups. Conclusion. Blood groups O and AB phenotypes are the most and the least ABO blood groups, respectively. There is significant association between ABO blood group and asymptomatic malaria parasitemia.

  12. A first-principles study of the possible magnetism of Rh in the Cu/Rh/Cu(001) system

    CERN Document Server

    Jang, Y R; Chang, C S; Cho, L H; Lee, J I

    1999-01-01

    Possible 4d magnetism of a Rh monolayer in a Cu/Rh/Cu(001) system is investigated using the full-potential linearized augmented-plane-wave (FLAPW) energy band method based on the local-spin-density approximation (LSDA). We have calculated the total energy of the Cu/Rh/Cu(001) system and have found that the Rh monolayer is ferromagnetic (FM) with a tiny magnetic moment. However, the total energy difference between the ferromagnetic and the paramagnetic states is found to be very small, and thus which state can be realized at room temperature is uncertain. The calculated charge densities and layer-projected density of states (LDOS) are presented and discussed in relation to the magnetic properties.

  13. Rh Incompatibility (For Parents)

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Rh Incompatibility KidsHealth > For Parents > Rh Incompatibility Print A ... this information early in your pregnancy. About the Rh Factor People with different blood types have proteins ...

  14. The affecting factors for the detection of RH blood type%影响RH检测的原因分析

    Institute of Scientific and Technical Information of China (English)

    危燕芬; 朱仕清; 汪丽娟

    2012-01-01

    Objective To explore the affecting factors for the detection of RH blood type and to ensure the labs to detect blood type rapidly and accurately by analyzing the methods of detecting RH blood type.Methods RH antigen was detected in the blood samples from 106 852 donators; the samples with negative RH ( D )were screened by normal saline and then re-tested by polybrene and indirect antihuman globulin.Results 316 of 106 852 ( 0.3% ) donators were RH ( D ) negative,3 of whom had Du subtype.Conclusions Normal saline for screening RH ( D ) negative blood samples,and indirect antihuman globulin and polybrene for confirming RH ( D ) negative can effectively ensure the accuracy of detection of RH blood type and has important significance of blood transfusion safety.%目的 通过分析RH血型检测方法,探讨影响RH检测结果的原因,确保实验室血型检测快捷、准确.方法 对106 852例本血站献血员标本进行RH (D)血型抗原检测,通过盐水法、聚凝胺法、间接抗人球蛋白法对RH阴性血进行初筛、复查.结果 受检者中检出RH (D)阴性316例,RH (D)阴性率为0.3%(316/106 852),其中检出RH Du亚型的3例.结论 盐水法对RH阴性血型进行筛查,间接抗人球蛋白法和聚凝胺法结合确证其阴性的检测,能有效保证RH血型检测的准确性,对输血安全有重要意义.

  15. Rh D blood group conversion using transcription activator-like effector nucleases

    Science.gov (United States)

    Kim, Young-Hoon; Kim, Hyun O.; Baek, Eun J.; Kurita, Ryo; Cha, Hyuk-Jin; Nakamura, Yukio; Kim, Hyongbum

    2015-01-01

    Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine. PMID:26078220

  16. Rh D blood group conversion using transcription activator-like effector nucleases.

    Science.gov (United States)

    Kim, Young-Hoon; Kim, Hyun O; Baek, Eun J; Kurita, Ryo; Cha, Hyuk-Jin; Nakamura, Yukio; Kim, Hyongbum

    2015-06-16

    Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine.

  17. 老年待输血患者Rh系统表型分型和不规则抗体检测及临床意义%Typing of Rh system phenotypes and detection of irregular antibody of elderly blood transfusion candidates and their significances

    Institute of Scientific and Technical Information of China (English)

    王晓宁; 王震; 刘冰; 金玉顺; 张冬霞

    2016-01-01

    Objective:To understand the typing of Rh system phenotypes and analyze the positive rate of irregular antibody in Rh system,and to provide basis for blood transfusion of the elderly patients in clinic. Methods:A total of 587 elderly blood tranfusion candidates were included.Microcolumn gel test was applied to detect the antigens of Rh system and identify the irregular antibody in Rh system.Results:There were 7 kinds of Rh blood group phenotypes with postive expression of RhD in 587 patients;the positive rates of irregular antibodies from high to low were CCDEE 38.7% (227/587),CcDEe 34.8% (204/587),CcDee 12.4% (73/587),ccDEE 8.7% (51/587),ccDEe 4.6% (27/587)and CCDEe 0.7% (4/587),ccDee 0.2% (1/587).There were 6 kinds of irregylar antibodies in 587 patients;the positive rate was 1.02%,including 4 cases of Anti-E (66.67%),1 case of Anti-c (16.67%), 1 case of anti-M (16.67%). The postive rate of irregular antibody in the patients with blood transfution history and pregnancy history (2.65%,51/89)was higher than those without blood transfusion history or pregnancy history 0.25%,13/98)(χ2=5.11,P0.05 ). Conclusion:The irregular antibody of Rh system is the major antibody in the eldery blood transtusion candidates.The detection of typing of Rh system phenotypes and identification of irregular antibodies in the patients with blood transfusion history and pregnancy history should be performed to ensure the safty of blood transfusion.%目的:了解老年待输血患者 Rh系统表型分型,分析其不规则抗体阳性率,为老年患者临床输血安全性提供依据。方法:选择老年待输血患者587例,采用微柱凝胶法对患者进行 Rh系统抗原检测及不规则抗体鉴定。结果:587例患者中测得 Rh血型表现型共7种,均为 RhD阳性, Rh血型表现型的频率由高到低分别为CCDee占38.7%(227/587)、CcDEe占34.7%(204/587)、CcDee占12.4%(73/587)、ccDEE占8.7%(51/587)、ccDEe占4.6%(27

  18. [Spectrophotometric characteristics of the amniotic fluid in Rh isoimmunization following intrauterine fetal blood transfusions].

    Science.gov (United States)

    Matrova, T S; Vasileva, Iu

    1989-01-01

    The amniotic fluids of 7 pregnant women with Rh-isoimmunization were examined. On the basis of the data of this investigation as well as of the clinical and ultrasound data intrauterine blood transfusions were made in fetuses--from 4 to 8 in number. Forty two spectrophotometric analyses were made in all, but the amniotic fluids were examined before intrauterine blood transfusions as well as before the performance of each subsequent blood transfusion. The authors found changes in the characteristic of the amniotic fluid after intrauterine blood transfusion, which were manifested by the fact that the pigment peak of delta 450 nm was reduced, but the peak of delta 410 nm was increased. In connection with these findings after intrauterine blood transfusions delta 450 nm lost its diagnostic and prognostic value. delta 410 nm before intrauterine blood transfusions manifested gravity of fetal hemolytic disease. After intrauterine blood transfusions its increase was due to blood transfusions and accumulation of methemoglobin in the amniotic fluid.

  19. DISTRIBUTION OF ABO AND RH-D BLOOD GROUPS IN THE CACHAR DISTRICT OF BARAK VALLEY OF ASSAM: IMPLICATION FOR REGIONAL BLOOD TRANSFUSION SERVICE

    Directory of Open Access Journals (Sweden)

    Dharmakanta Kumbhakar

    2016-07-01

    Full Text Available BACKGROUND ABO and Rhesus (Rh-D blood group antigens are integrated parts of the red blood cell membranes. They are hereditary characters and are useful in population genetic studies, in resolving medico-legal issues and more importantly in compatibility test in blood transfusion and organ transplant practices. They show a wide geographical and racial variation. The knowledge of the distribution of ABO and Rh-D blood groups among different population is essential in health care and transfusion practices. AIMS AND OBJECTIVES To study the distribution of ABO and Rh-D blood groups amongst the population of Cachar district of Barak valley of Assam. MATERIALS AND METHODS We did a retrospective analysis of records of 1,60,500 blood samples grouped for ABO and Rh-D typing at Silchar Medical College and Hospital Blood Bank, Silchar, over a period of 10 years from 1st January 1999 to 31st December 2008. RESULTS AND OBSERVATIONS Out of total 1,60,500 blood samples grouped for ABO and Rh-D typing during the period in the centre, the distribution of phenotype A, B, AB and O were 24.80% (39,804, 32.00% (51,360, 5.60% (8,986 and 37.60% (60,350 respectively. The Rh-D positive phenotype was 95.40% (1, 53,117 and remaining 4.60% (7,383 was Rh-D negative. The frequency of Rh-D phenotypes in the various ABO blood groups was as - A Positive 23.70% (38,039, A Negative 1.10% (1,765, B Positive 30.80% (49,433, B Negative 1.20% (1,927, AB Positive 5.40% (8,665, AB Negative 0.20% (321, O Positive 35.50% (56,980 and O Negative 2.10% (3,370 respectively. DISCUSSION Silchar Medical College and Hospital Blood Bank receives blood samples for grouping of almost all population of Cachar district. Hence, the data revealed in the present study fairly reflects the prevalence of ABO and Rh-D groups distribution in the Cachar district in Barak valley of Assam. CONCLUSION The present study provide information on the status of ABO and Rh-D blood groups distribution of the region and

  20. Molecular matching for Rh and K reduces red blood cell alloimmunisation in patients with myelodysplastic syndrome

    Science.gov (United States)

    Guelsin, Gláucia A.S.; Rodrigues, Camila; Visentainer, Jeane E.L.; de Melo Campos, Paula; Traina, Fabíola; Gilli, Simone C.O.; Saad, Sara T.O.; Castilho, Lilian

    2015-01-01

    Background Matching for Rh and K antigens has been used in an attempt to reduce antibody formation in patients receiving chronic transfusions but an extended phenotype matching including Fya and Jka antigens has also been recommended. The aim of this study was to identify an efficient transfusion protocol of genotype matching for patients with myelodysplastic syndrome (MDS) or chronic myelomonocytic leukaemia. We also examined a possible association of HLA class II alleles with red blood cell (RBC) alloimmunisation. Materials and methods We evaluated 43 patients with MDS undergoing transfusion therapy with and without antibody formation. We investigated antigen-matched RBC units for ABO, D, C, c, E, e, K, Fya, Fyb, Jka, Jkb, S, s, Doa, Dob and Dia on the patients’ samples and on the donor units serologically matched for them based on their ABO, Rh and K phenotypes and presence of antibodies. We also determined the frequencies of HLA-DRB1 alleles in the alloimmunised and non-alloimmunised patients. Results Seventeen of the 43 patients had discrepancies or mismatches for multiple antigens between their genotype-predicted profile and the antigen profile of the units of blood serologically matched for them. We verified that 36.8% of patients had more than one RBC alloantibody and 10.5% of patients had autoantibodies. Although we were able to find a better match for the patients in our extended genotyped/phenotyped units, we verified that matching for Rh and K would be sufficient for most of the patients. We also observed an over-representation of the HLA-DRB1*13 allele in the non-alloimmunised group of patients with MDS. Discussion In our population molecular matching for C, c, E, e, K was able to reduce RBC alloimmunisation in MDS patients. An association of HLA-DRB1*13 and protection from RBC alloimmunisation should be confirmed. PMID:24960644

  1. Rh阴性患者输血策略%The transfusion strategy for patients with Rh negative blood

    Institute of Scientific and Technical Information of China (English)

    兰炯采

    2015-01-01

    近年来,有关Rh阴性患者的输血问题由于媒体炒作而变得复杂化,不利于临床对患者的生命抢救。政府已对Rh阴性患者的输血颁布了有关法规,以指导临床安全、有效和科学的输血。对于体内无抗-D抗体的Rh阴性患者,在血资源紧急情况下可以输配血相合的Rh阳性血,而对于有抗-D抗体者则应尽量输注Rh阴性血。本文阐述Rh阴性患者安全、有效、科学的输血策略。%In recent years, the blood transfusion in Rh negative patients has been complicated due to media hype, which hampers the patients’ life in clinical emergency treatment. The government has already issued the relevant laws and regulations for Rh negative blood transfusion in order to guide the safe, effective and scientiifc blood transfusion. When the blood resources are insuifcent in an emergency situation, the Rh negative patients without anti-D antibody can receive Rh D-positive blood, while those with anti-D antibody must infuse Rh D negative blood as possible. This paper states the strategy for safe, scientiifc and reasonable blood transfusion in patients with Rh negative blood.

  2. Study on Database of RhD-Negative Blood Group in Umbilical Cord Blood%新生儿脐血RhD阴性稀有血型资料库的建立研究

    Institute of Scientific and Technical Information of China (English)

    粱晓岚; 李茜; 孙乐静; 杜颖; 刘兰婷; 刘剑; 武文杰; 邱录贵

    2011-01-01

    Objective A RhD-negative blood group database was established in Tianjin CBB to explore the distribution of RhD-negative blood group.Methods 154 882 cases of neonatal health UCB samples as the research object were collected between October 2001 and August 2011 of Tianjin CBB.The ABO blood group antigen and RhD antigen were detected by standard tube agglutination test.RhD-negative was confirmed by indirect antiglobulin test.Results 581(0.37%)RhD-negative cases were detected in total of 154 882 UCB samples,including type A 137 cases(23.58%),type B 191cases(32.87%),type O 195 cases(33.56%)and type AB 58 cases(9.98%),mainly 51.98% in males and 48.02% in females.The major antigen phenotypes of RhD-negative blood type were ccdee and Ccdee phenotype(81.67%).Conclusion With RhD-negative blood group database of neonate umbilical cord blood established,the reference data of scientific blood use,clinical prophylaxis and therapy would be provided.%目的 建立新生儿脐血RhD阴性稀有血型资料库,了解天津脐血库存储人群Rh血型分布情况.方法 收集2001年10月至2011年8月天津脐血库存储的154 882例健康新生儿脐血样本作为研究对象,使用试管法检测样本ABO抗原和RhD抗原,RhD阴性确认采用间接抗人球蛋白试验.结果 154882例脐血样本中检出RhD阴性581例(0.37%),其中A型137例(23.58%)、B型191例(32.87%)、O型195例(33.56%)、AB型58例(9.98%);男性和女性分别占51.98%和48.02%,RhD阴性的表型以ccdee和Ccdee为主(81.67%).结论 建立新生儿脐血RhD阴性稀有血型资料库,可为RhD阴性群体的科学用血与临床预防、治疗提供参考数据.

  3. A conserved non-reproductive GnRH system in chordates.

    Directory of Open Access Journals (Sweden)

    Takehiro G Kusakabe

    Full Text Available Gonadotropin-releasing hormone (GnRH is a neuroendocrine peptide that plays a central role in the vertebrate hypothalamo-pituitary axis. The roles of GnRH in the control of vertebrate reproductive functions have been established, while its non-reproductive function has been suggested but less well understood. Here we show that the tunicate Ciona intestinalis has in its non-reproductive larval stage a prominent GnRH system spanning the entire length of the nervous system. Tunicate GnRH receptors are phylogenetically closest to vertebrate GnRH receptors, yet functional analysis of the receptors revealed that these simple chordates have evolved a unique GnRH system with multiple ligands and receptor heterodimerization enabling complex regulation. One of the gnrh genes is conspicuously expressed in the motor ganglion and nerve cord, which are homologous structures to the hindbrain and spinal cord of vertebrates. Correspondingly, GnRH receptor genes were found to be expressed in the tail muscle and notochord of embryos, both of which are phylotypic axial structures along the nerve cord. Our findings suggest a novel non-reproductive role of GnRH in tunicates. Furthermore, we present evidence that GnRH-producing cells are present in the hindbrain and spinal cord of the medaka, Oryzias latipes, thereby suggesting the deep evolutionary origin of a non-reproductive GnRH system in chordates.

  4. Bases moleculares do sistema Rh e suas aplicações em obstetrícia e medicina transfusional The molecular basis of RH system and its applications in obstetrics and transfusion medicine

    Directory of Open Access Journals (Sweden)

    Luciano Marcondes Machado Nardozza

    2010-01-01

    Full Text Available O sistema Rh é o mais polimórfico e imunogênico de todos os sistemas de grupos sanguíneos. Atualmente mais de 49 antígenos foram identificados sendo cinco principais os antígenos D, C, c, E, e. O conhecimento das bases moleculares do sistema Rh desde a sua primeira clonagem há 17 anos possibilitou o entendimento tanto do mecanismo do fenótipo Rh negativo quanto das variantes dos antígenos RHD e RHCE. As deleções, rearranjos gênicos e as inserções são as principais mutações encontradas. Nos caucasianos, o mecanismo principal do fenótipo Rh negativo é a completa deleção do gene RHD, enquanto nos afrodescendentes é a presença do pseudogene RHDψ e do gene híbrido RHD-CE (4-7-D. Os autores analisam a estrutura do complexo Rh nas hemácias, as bases moleculares do Sistema Rh, os mecanismos de negatividade RHD, além da Expressão fraca e parcial de D.The Rh system is the most polymorphic and immunogenic for all blood group systems. Currently more than 49 antigens were identified with five major antigens D, C, c, E, e. Knowledge of the Rh system's molecular basis, since its first cloning 17 years ago, allowed to understand the mechanism of Rh-negative phenotype and the variants of antigens as RHD and RHCE. Deletions, gene rearrangements and insertions are the main mutations. In Caucasians the primary mechanism of Rh-negative phenotype is the complete RHD gene deletion, while in African descendants it is the presence of pseudogene and gene RHDψ hybrid RHD-CE (4-7-D. The authors analyze the structure of the Rh complex in red cells, molecular basis of the Rh system, mechanisms of Negativity RHD and weak and incomplete expression of RHD.

  5. Prevalence of Rh, Duffy, Kell, Kidd & MNSs blood group antigens in the Indian blood donor population

    Directory of Open Access Journals (Sweden)

    R N Makroo

    2013-01-01

    Interpretation & conclusions: This study found the prevalence of the typed antigens among Indian blood donors to be statistically different to those in the Caucasian, Black and Chinese populations, but more similar to Caucasians than to the other racial groups.

  6. RhC Phenotyping, Adsorption/Elution Test, and SSP-PCR: The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors.

    Science.gov (United States)

    Srijinda, Songsak; Suwanasophon, Chamaiporn; Visawapoka, Unchalee; Pongsavee, Malinee

    2012-01-01

    The Rhesus (Rh) blood group is the most polymorphic human blood group and it is clinically significant in transfusion medicine. Especially, D antigen is the most important and highly immunogenic antigen. Due to anti-D, it is the cause of the hemolytic disease of the newborn and transfusion reaction. About 0.1%-0.5% of Asian people are RhD-negative, whereas in the Thai population, the RhD-negative blood type only occurs in 0.3%. Approximately 10%-30% of RhD-negative in Eastern Asian people actually were D-elute (DEL) phenotype, the very weak D antigen that cannot be detected by indirect antiglobulin test (IAT). There are many reports about anti-D immunization in RhD-negative recipients through the transfusion of red blood cells from individuals with DEL phenotype. D-elute phenotype screening in Thai RhD-negative blood donors was studied to distinguish true RhD-negative from DEL phenotype. A total of 254 Thai serologically RhD-negative blood donors were tested for RhCE phenotypes and anti-D adsorption/elution test. In addition, RhC(+) samples were tested for RHD 1227A allele by SSP-PCR technique. The RhD-negative phenotype samples consisted of 131 ccee, 4 ccEe, 1 ccEE, 101 Ccee, 16 CCee, and 1 CcEe. The 42 Ccee and 8 CCee phenotype samples were typed as DEL phenotype and 96% of DEL samples were positive for RHD 1227A allele. The incidence of RhC(+) was 46.4%, and 48 of the 118 RhC(+) samples were positive for both anti-D adsorption/elution test and SSP-PCR technique for RHD 1227A allele. The sensitivity and specificity were 96% and 100%, respectively, for RHD 1227A detection as compared with the adsorption/elution test. In conclusion, RhC(+) phenotype can combine with anti-D adsorption/elution test and RHD 1227A allele SSP-PCR technique for distinguishing true RhD-negative from DEL phenotype.

  7. Blood

    Science.gov (United States)

    ... Also, blood is either Rh-positive or Rh-negative. So if you have type A blood, it's either A positive or A negative. Which type you are is important if you need a blood transfusion. And your Rh factor could be important ...

  8. Frequency of ABO and Rh (D Blood Groups and Hemoglobin Threshold among Pregnant Women in Family Guidance Association, Mekelle Model Clinic, North Ethiopia.

    Directory of Open Access Journals (Sweden)

    Megbaru Alemu

    2014-12-01

    Full Text Available This study was aimed to determine the frequency of ABO and Rh blood group patterns and hemoglobin threshold among pregnant women screened at Family Guidance Association of Ethiopia, North Area Mekelle model clinic. Checklists were prepared to collect data from laboratory registration books of five years to undergo this retrospective study. Those registration books were reviewed for ABO blood group, Rh profiles and hemoglobin threshold of the pregnant women screened during the specified period. A total of 5987 pregnant women have been found to be screened and the predominant phenotype in this study was O (41.5% followed by phenotype A (28%, Phenotype B (25% and phenotype AB (5.5%. Regarding Rh (D system, 91.2% (5458/5987 women were positive for rhesus D antigen while the remaining 8.8% (529/5987 women were negative for the antigen. Blood grouping studies should be done in every region of the nation so that national transfusion policies can be drafted and supplying blood to the needy patients during emergency will be easier.

  9. A novel paper-based assay for the simultaneous determination of Rh typing and forward and reverse ABO blood groups.

    Science.gov (United States)

    Noiphung, Julaluk; Talalak, Kwanrutai; Hongwarittorrn, Irin; Pupinyo, Naricha; Thirabowonkitphithan, Pannawich; Laiwattanapaisal, Wanida

    2015-05-15

    We propose a new, paper-based analytical device (PAD) for blood typing that allows for the simultaneous determination of ABO and Rh blood groups on the same device. The device was successfully fabricated by using a combination of wax printing and wax dipping methods. A 1:2 blood dilution was used for forward grouping, whereas whole blood could be used for reverse grouping. A 30% cell suspension of A-cells or B-cells was used for haemagglutination on the reverse grouping side. The total assay time was 10 min. The ratio between the distance of red blood cell movement and plasma separation is the criterion for agglutination and indicates the presence of the corresponding antigen or antibody. The proposed PAD has excellent reproducibility in that the same blood groups, namely A, AB, and O, were reported by using different PADs that were fabricated on the same day (n=10). The accuracy for detecting blood group A (n=12), B (n=13), AB (n=9), O (n=14), and Rh (n=48) typing were 92%, 85%, 89%, 93%, and 96%, respectively, in comparison with the conventional slide test method. The haematocrit of the sample affects the accuracy of the results, and appropriate dilution is suggested before typing. In conclusion, this study proposes a novel method that is straightforward, time-saving, and inexpensive for the simultaneous determination of ABO and Rh blood groups, which is promising for use in developing countries. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. RH-TB精炼工艺优化%Process Optimization for RH-TB Refining System

    Institute of Scientific and Technical Information of China (English)

    于华财; 宋满堂; 林东

    2005-01-01

    通过对本钢炼钢厂的RH-TB真空精炼工艺的优化,使真空泵系统最低真空度达到了67 Pa,实现了IF钢等超低碳钢的稳定生产.真空罐耐材的使用寿命显著提高,尤其RH吸嘴的使用寿命由原平均35次提高到平均104.5次.

  11. Frequencies of polymorphisms of the Rh, Kell, Kidd, Duffy and Diego systems of Santa Catarina, Southern Brazil

    Directory of Open Access Journals (Sweden)

    Daiane Cobianchi Costa

    Full Text Available ABSTRACT BACKGROUND: Red blood cell genes are highly polymorphic with the distribution of alleles varying between different populations and ethnic groups. The objective of this study was to investigate gene polymorphisms of blood groups in the state of Santa Catarina, Southern Brazil. METHODS: Three hundred and seventy-three unrelated blood donors and 31 transfusion-dependent patients were evaluated to investigate polymorphisms of the Rh, Kell, Duffy, Kidd, and Diego blood group systems in a population from the state of Santa Catarina. The subjects, from seven regions that comprise the blood-banking network of the state, were assessed between August 2011 and March 2014. The genotypes of the Rh, Kell, Duffy, Kidd, and Diego systems were determined using the restriction fragment length polymorphism-polymerase chain reaction and allele-specific polymerase chain reaction techniques. RESULTS: The genotype frequencies in this study were significantly different when populations from different regions of Santa Catarina were compared. Furthermore, there were also significant differences in the genetic frequencies compared to other Brazilian states. The genotype frequencies of the Kell and Kidd blood groups are similar to European populations from Naples, Italy and Zurich, Switzerland. CONCLUSION: This article reports for the first time the frequency of polymorphisms of blood group systems in blood donors from Santa Catarina, Southern Brazil.

  12. The Popularization and Application of Cold Storage Red Blood Cells or Whole Blood at -80 ℃ of the Rh(D) Negative Patients in Surgical Operation

    Institute of Scientific and Technical Information of China (English)

    余忠清; 胡丽华; 韩敏; 饶神宗; 罗成伟

    2002-01-01

    Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients re-ceived the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K+, Na+, BUN, Cr, urine protein (URPO), UOB, Hb,HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfu sion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0. 05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K+ and blood Na+ was normal with the difference being not significant before and after operation (P> 0. 05). Plasma protein was decreased,but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh (D) negative patients without side effects during the surgical operation.

  13. Evaluation of fibronectin 1 in one dried blood spot and in urine after rhGH treatment.

    Science.gov (United States)

    Ferro, P; Ventura, R; Pérez-Mañá, C; Farré, M; Segura, J

    2016-10-07

    Since the appearance of recombinant human growth hormone (rhGH) in the 1980s, its expansion and acquisition through the black market has increased, so the detection of its abuse continues to be a challenge. New biomarkers that are more reliable and sensitive, allowing a larger detection window, are still needed. In this line, Fibronectin 1 (FN1) has been proposed as a potential genetic and protein biomarker of rhGH abuse in peripheral blood lymphocytes, serum, and plasma. However, logistic problems associated with current blood collection in sports drug testing point towards potential new alternative matrices that could be good candidates to be evaluated. Results obtained in this study showed high ELISA FN1 levels in one dried blood spot and in urine samples in ten healthy male volunteers treated with rhGH. Results showed that especially dried blood spots appear as a potential good matrix to detect rhGH abuse by means of FN1 biomarker. Copyright © 2016 John Wiley & Sons, Ltd.

  14. Frequencies and ethnic distribution of ABO and Rh(D) blood groups in Mauritania: results of first nationwide study.

    Science.gov (United States)

    Hamed, C T; Bollahi, M A; Abdelhamid, I; Med Mahmoud, M A; Ba, B; Ghaber, S; Habti, N; Houmeida, A

    2012-04-01

    There is no data available on the ABO/Rh(D) frequencies in the Mauritanian population. We retrospectively analysed records of a 5-year database that contained ABO/Rh phenotype and ethnic origin of 10 116 volunteers giving blood at the national blood transfusion centre to derive the frequencies of ABO/Rh(D) groups in the Mauritanian population. The two race categories in the country and their sub-ethnic groups: the Moors (whites and black) and the black Africans (Pulhars, Soninkes and Wolof) were included in this study. Globally, group O had the highest frequency (49.10%) followed by A (28.28%), B (18.56%) and AB (4.05%). This order more common in North African populations was found in four of the five ethnic groups composing our population. Allele frequencies were, respectively, 70.20%, 17.74% and 12.04% giving the same order of O > A > B. We observed no significant variation in these frequencies between the different ethnic groups. Rhesus study showed that with a percentage of 94.23% Rh(D) positive is by far the most prevalent, while Rh(D) negative is present only in 5.77% of the total population. This frequency distribution supports the mixed-race composition of the Mauritanian population. © 2011 Blackwell Publishing Ltd.

  15. The Distribution of ABO and RhD Negative Phenotypes of Blood Donors in Xinxiang District%新乡地区无偿献血者ABO及RhD阴性表型分布

    Institute of Scientific and Technical Information of China (English)

    孟毓; 岳献荣; 曹珺; 秦小敏

    2015-01-01

    Objective To investigate in Xinxiang area blood donors of RhD Negative blood distribution of ABO blood group and RhD Negative phenotype, and constantly improve the RhD Negative blood donation are database and guarantee the clinical safe use of blood. Methods This study will have a statistical analysis RhD negative blood donors ABO blood type distribution、 RhD negative blood donors antigen phenotypes distribution and Clinical use and blood scrapped situation of RhD negative blood from January 2010 to March 2015. Results RhD negative rate in Voluntary Blood Donors is 0.50% in Xinxiang District, the distribution of ABO blood group was B (33.19%) >A (29.37%) >O (27.29%) >AB (10.15%), and the composition of blood group was lower than that in the sequence, there are significant differences in the differences between different blood groups (PdCcee> dccEe> dCCee>dCcEe>dccEE ,There are significant differences in the differences between different antigen phenotypes(PO (29.37%)>A (27.29%)>AB(10.15%),不同血型间差异有统计学意义(PdCcee>dccEe>dCCee>dCcEe>dccEE,不同抗原表型间差异有统计学意义(P<0.01)。结论通过对新乡地区2010年1月-2015年3月无偿献血者ABO血型及RhD阴性表型分布进行统计分析,有针对性地制定招募方案,同时完善了RhD阴性献血者表型分型档案库,为临床用血者提供RhD表型相合的血液,对临床输血的安全性和有效性意义重大。

  16. Blood groups systems

    Directory of Open Access Journals (Sweden)

    Ranadhir Mitra

    2014-01-01

    Full Text Available International Society of Blood Transfusion has recently recognized 33 blood group systems. Apart from ABO and Rhesus system, many other types of antigens have been noticed on the red cell membranes. Blood grouping and cross-matching is one of the few important tests that the anaesthesiologist orders during perioperative period. Hence, a proper understanding of the blood group system, their clinical significance, typing and cross-matching tests, and current perspective are of paramount importance to prevent transfusion-related complications. Nonetheless, the knowledge on blood group system is necessary to approach blood group-linked diseases which are still at the stage of research. This review addresses all these aspects of the blood groups system.

  17. In vitro generated Rh(null) red cells recapitulate the in vivo deficiency: a model for rare blood group phenotypes and erythroid membrane disorders.

    Science.gov (United States)

    Cambot, Marie; Mazurier, Christelle; Canoui-Poitrine, Florence; Hebert, Nicolas; Picot, Julien; Clay, Denis; Picard, Véronique; Ripoche, Pierre; Douay, Luc; Dubart-Kupperschmitt, Anne; Cartron, Jean-Pierre

    2013-05-01

    Lentiviral modification combined with ex vivo erythroid differentiation was used to stably inhibit RhAG expression, a critical component of the Rh(rhesus) membrane complex defective in the Rh(null) syndrome. The cultured red cells generated recapitulate the major alterations of native Rh(null) cells regarding antigen expression, membrane deformability, and gas transport function, providing the proof of principle for their use as model of Rh(null) syndrome and to investigate Rh complex biogenesis in human primary erythroid cells. Using this model, we were able to reveal for the first time that RhAG extinction alone is sufficient to explain ICAM-4 and CD47 loss observed on native Rh(null) RBCs. Together with the effects of RhAG forced expression in Rh(null) progenitors, this strongly strengthens the hypothesis that RhAG is critical to Rh complex formation. The strategy is also promising for diagnosis purpose in order to overcome the supply from rare blood donors and is applicable to other erythroid defects and rare phenotypes, providing models to dissect membrane biogenesis of multicomplex proteins in erythroid cells, with potential clinical applications in transfusion medicine.

  18. 长沙市某三甲医院住院患者RhD阴性血型分布特征*%Distribution of RhD negative blood group of inpatients in a first-class grade-three hospital in Changsha

    Institute of Scientific and Technical Information of China (English)

    黄琬婷; 覃婉元; 陈廷寅; 周俭辉; 沈亚梅; 李碧娟; 李宁

    2016-01-01

    目的:探讨长沙地区某三甲医院住院患者RhD阴性血型分布特征。方法用血型凝胶卡和试管法对2011年1月-2015年4月的住院患者进行ABO及RhD血型检测。结果255179例住院患者ABO血型分布特征为O>A>B>AB,男女之间ABO血型分布差异无统计学意义(>0.05);检出RhD阴性个体1470例,阴性率为0.58%,其中A型RhD阴性[A(-)]占0.22%,B(-)占0.12%,AB(-)占0.04%,O(-)占0.20%,分布特征为A>O>B>AB;男女之间RhD阴性分布差异有统计学意义(O>A>AB,女性为A>O>AB>B。RhD阴性患者中以产科患者为首,约占28.23%,其次为普外科患者和神经外科患者,分别占16.63%和7.91%。结论住院患者RhD阴性血型具有较高的频率,女性高于男性,且以产科患者为主。RhD阴性血型在ABO血型系统中的分布存在性别差异,其机制有待进一步研究。%Objective To investigate RhD negative blood type distribution of inpatients in a first-class grade-three hospital in Changsha. Methods The DiaMed gel card and test tube methods were used to detect blood type of patients from January of 2011 to April of 2015. Results A survey of blood samples of 255,179 inpatients showed the distribution of O>A>B>AB, and there was no significant distribution difference between female and male (P>0.05). Among those patients, there were 1,470 RhD negative individuals, which accounted for 0.58% in all inpatients. Blood group A RhD negative [A (- )] had the highest distribution of 0.22%, followed by O(-) and B(-) with 0.20% and 0.12% respectively, and the lowest distribution of 0.04% was AB (-). The RhD negative ratio of female was higher than that of male. RhD negative distribution in ABO system was different between male and female; the order in male was B>O>A>AB, while the order in female was A>O>AB>B. Among all RhD negative patients, the majority of the patients were obstetric patients accounting for 28.23%, followed by neurosurgical

  19. RhD Negative Red Blood Cells Confivmed Associated with Clinical Transfusion XIA%RhD红细胞阴性确认与临床输血的关联

    Institute of Scientific and Technical Information of China (English)

    夏富益

    2015-01-01

    Objective Research and analysis the results of confirming RhD negative red blood cel s,toexplore the significance of RhD antigent in clinical transfusion. Methods By using saline medium method to experiment 217 RhD negative samples from blood donors in blood testing Laboratory again,then by indirect antiglobulin test to detect weak and part D. Results 212 of 217 samples were RhD negative (97.70%),and 5 samples were identified as weak D or part D (2.30%). Conclusion RhD antigens is complicated and also with a variation of characteristics, the confirm of RhD negative red blood cel s and to pvevent the risk of blood transfusion are associated with the hemolytic transfusion reaction which caused by RhD antigen.%目的研究分析RhD红细胞阴性确认实验结果,探讨RhD抗原在临床输血工作中的重要意义。方法血站检测实验室217例无偿献血者初筛为阴性的标本用盐水介质法进行复初筛,对复初筛为阴性的标本采用间接抗球蛋白试验检测出弱D和部分D表型。结果217例初筛阴性的标本检出212例为RhD(-)(97.70豫),5例确定为弱D或部分D型(2.30豫)。结论 RhD抗原呈复杂变异性,RhD红细胞阴性确认与防范输血风险,避免由RhD抗原引起的溶血性输血反应息息相关。

  20. A thermodynamic description of the system Pd-Rh-H-D-T

    Energy Technology Data Exchange (ETDEWEB)

    Joubert, J.-M., E-mail: jean-marc.joubert@icmpe.cnrs.fr [Chimie Metallurgique des Terres Rares, Institut de Chimie et des Materiaux Paris-Est, CNRS, Universite Paris-Est, UMR 7182, 2-8 Rue Henri Dunant, F-94320 Thiais (France); Thiebaut, S. [CEA/DAM/Valduc, F-21120 Is sur Tille (France)

    2011-02-15

    The quinary system D-H-Pd-Rh-T has been described thermodynamically by the CALPHAD approach. Previous descriptions of the binary subsystems have been used. To model the high pressure data an equation of state for the gases D{sub 2} and T{sub 2} compatible with the CALPHAD approach has been obtained similar to that previously used for H{sub 2}. A complete literature search has been undertaken for the three ternary systems H-Pd-Rh, D-Pd-Rh and Pd-Rh-T and the most significant experimental data have been selected for a thermodynamic assessment of these systems. In order to complement the available data, pressure-composition curves have been measured at different temperatures for the two last systems in the present work. Calculations and optimization of the system under para-equilibrium conditions, i.e. in pseudo-binary systems (Pd,Rh)-H, (Pd,Rh)-D or (Pd,Rh)-T, have been achieved using a pseudo-atom describing the Pd-Rh solid solution. This special method allows the presence of a miscibility gap in the binary metallic system to be dealt with. We show that a simple combination of the binary systems alone is unable to properly describe these ternary systems and that ternary interaction parameters have to be introduced. The binary and ternary systems may then be combined to perform calculations in the quinary D-H-Pd-Rh-T system. It is believed that extrapolation in systems containing different isotopes are fairly accurate provided that the so-called Toop model is used.

  1. Association between ABO and Rh Blood Groups and Risk of Preeclampsia: A Case-Control Study from Iran.

    Science.gov (United States)

    Aghasadeghi, Firoozeh; Saadat, Mostafa

    2017-04-15

    Preeclampsia (PE) is a major cause of maternal and neonatal morbidity and mortality. There is a genetic component in the development of PE with estimated heritability around 0.47. Several studies have investigated the association between maternal ABO blood groups (OMIM 110300) and risk of PE, with contradictory results have emerged. Considering that there is no study in this filed from Iranian population, the present case-control study was carried out at Shiraz (south-west Iran). In this study 331 women; 121 pregnant with PE and 210 normotensive pregnant women were included. Using blood group O (for ABO blood groups) or Rh+ (for Rh blood groups) as a reference, odds ratios (ORs) and its 95% confidence intervals (95% CI) of PE risk were estimated from logistic regression analysis. Although the A (OR = 0.67, 95% CI = 0.39-1.17, P = 0.165), B (OR = 0.86, 95% CI = 0.48-1.53, P = 0.615) and AB (OR = 1.14, 95% CI = 0.37-3.45, P = 0.812) phenotypes showed lower risks compared with the O blood group, statistical analysis indicated that there was no significant association between ABO phenotypes and risk of PE. The frequency of Rh- phenotype was higher among PE patients compared with the control group. However, the association was not significant (OR = 1.79, 95% CI = 0.69-4.65, P = 0.229). Adjusted ORs for age of participants and parity did not change the above-mentioned associations. Our present findings indicate that there is no association between ABO and Rh blood groups and risk of PE in Iranian population.

  2. Rh System and Intrauterine Growth. Interaction with Season of Birth

    Directory of Open Access Journals (Sweden)

    F. Gloria-Bottini

    2000-01-01

    Full Text Available Based on the hypothesis that maternal-fetal genetic differences in membrane transport and signal transduction may influence intrauterine development, the recent acquisition on transport function of Rh protein prompted us to study the relationship between joint maternal-fetal Rh phenotype and birth weight. Considering that metabolic effect of maternal-fetal competition could be amplified by environmental conditions, we have investigated possible seasonal effects on such relationship. We have studied 5291 infants born in Sardinia in the period January 1993–December 1996 and 984 infants born in Rome during 1996. In Rh(– mothers there is a significant association between season of birth and birth weight that shows the highest mean value in infants born in autumn (i.e. conceived in winter. The association is much more evident in male than in female infants. In male infants from Rh(– mothers, the association between birth weight and season is significant in Rh(+ male newborns only. Recent observations by our group in NIDDM suggest that glucose transport in RBC may be related to D protein, thus we propose an interpretation of the present observation in terms of transport function. When the density of D protein in the infant is greater than in the mother, the balance is in favour of the infant who may attain a significant developmental advantage when conceived in the cold season.

  3. ABO and RH1 blood group phenotyping in pigs (Sus scrofa) using microtyping cards.

    Science.gov (United States)

    Martínez-Alarcón, L; Ramis, G; Majado, M J; Quereda, J J; Herrero-Medrano, J M; Ríos, A; Ramírez, P; Muñoz, A

    2010-01-01

    Transplantation or transfusion with ABO disparity is a cause for rejection or for severe hemodynamic alterations. ABO groups in pigs are commonly an unknown variable, which has been previously assessed by means of hemagglutination tests or immunohistochemical procedures on tissues. Herein, we have reported a simple method using commercial microcards for human ABO typing. However, the reagents directly derived from human sera included in these cards can result in false determinations due to alpha-gal interference. The ABO groups of 19 wild-type pigs (Landrace x Large White) were assessed using 2 commercial cards: Human sera-based and monoclonal antibody-based cards. The human sera cards determined that 8 pigs belonged to the AB group and 11 to the B group. The monoclonal antibody cards determined that 8 pigs belonged to the A group and 11 to the O group. None of the pigs showed reactions to Rh1 antibodies. Because the B group has not been described in pigs, the reaction in human sera cards represented an interference with alpha-gal antigen, a molecule structurally similar to the B blood antigen. Thus, microtyping cards based on monoclonal antibodies provided simple, quick way to assess ABO groups in pigs used for xenotransplantation. ABO concordance should always be investigated for these types of procedures.

  4. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran

    Science.gov (United States)

    MOHAMMADALI, Fatemeh; POURFATHOLLAH, Aliakbar

    2014-01-01

    Abstract Background The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC), and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011. Methods This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical difference between the values among different categories according to SPSS program. Results Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%, 0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of donors (P 0.05). Conclusion Compared with neighboring countries and the international standards, prevalence of blood-borne infections is relatively low. PMID:25909065

  5. Association of ABO and Rh Blood Groups to Blood-Borne Infections among Blood Donors in Tehran-Iran.

    Directory of Open Access Journals (Sweden)

    Fatemeh Mohammadali

    2014-07-01

    Full Text Available The aim of this study was to investigate the prevalence of hepatitis B, hepatitis C, HIV and syphilis infections in blood donors referred to Tehran Blood Transfusion Center (TBTC, and determine any association between blood groups and blood- borne infections between the years of 2005 and 2011.This was a retrospective study conducted at TBTC. All of the donor serum samples were screened for HBV, HCV, HIV and syphilis by using third generation ELISA kits and RPR test. Initial reactive samples were tested in duplicate. Confirmatory tests were performed on all repeatedly reactive donations. Blood group was determined by forward and reverse blood grouping. The results were subjected to chi square analysis for determination of statistical difference between the values among different categories according to SPSS program.Overall, 2031451 donor serum samples were collected in 2005-2011. Totally, 10451 were positive test for HBV, HCV, HIV and syphilis. The overall seroprevalence of HBV, HCV, HIV, and syphilis was 0.39%, 0.11%, 0.005%, and 0.010%, respectively. Hepatitis B and HIV infections were significantly associated with blood group of donors (P 0.05.Compared with neighboring countries and the international standards, prevalence of blood-borne infections is relatively low.

  6. Cryopreservation of Rh negative blood for improved storage & utilisation by means of indigenous freezing bags & solutions & manual deglycerolisation.

    Science.gov (United States)

    Nanu, A; Lal, M

    2001-04-01

    Cryopreservation allows donor blood to be stored for years, rather than weeks as in liquid storage. This is an established procedure in countries with a developed blood transfusion service. Cryopreservation has not been introduced in India, possibly because of the presumed high cost and complexity of the procedure. An attempt is made in this study, to cryopreserve Rh negative blood in a mechanical freezer using indigenous bags and solutions and manual deglycerolisation to reduce the cost of preservation. RBCs to be frozen were weighed and transferred by sterile welding to an 800 ml freezing bag. Based on RBC weight, 400-500 ml of glycerol (5.7 MpH 6.8) was added as cryopreservative. After extracellular and intracellular equilibration, excess glycerol was removed by centrifugation. Glycerolised RBCs were frozen at -80 degrees C in a mechanical freezer. Cryopreserved units were thawed at 37 degrees C and deglycerolised. Twenty one patients were transfused with the frozen deglycerolised RBCs after the usual pre-transfusion tests. Haematocrit (Hct) values of the frozen deglycerolised units, complied with required standards, before addition of glycerol and post glycerolisation. RBC recovery was more than 80 per cent and Hct 83.3 per cent after deglycerolisation. Twenty one patients transfused with the frozen deglycerolised blood experienced no adverse effects and showed a 24 h mean post transfusion Hb increment of 0.73 g/dl. Cryopreservation, unlike liquid storage, allows Rh negative blood of the required ABO groups to be stocked. Cryopreservation also improves its utilisation, reduces wastage and helps supplement the liquid stocks of Rh negative blood. The use of indigenous freezing bags and manual deglycerolisation has resulted in an inexpensive procedure, which can be integrated, with minor inputs, into any blood bank that is already preparing blood components.

  7. Ontogeny of the fetal immune system: study on pregnancies with Rh-isoimmunization and nonimmune fetal hydrops.

    Science.gov (United States)

    Noia, G; Romano, D; De Santis, M; Gozzo, M L; Colacicco, L; Mariorenzi, S; Straface, G; Rumi, C; Caruso, A; Mancuso, S

    1999-01-01

    This study aims at observing and comparing the antigen expression of some fetal T- and B-lymphocyte subpopulations in Rh-isoimmunization, which determines anemic hypoxia in the fetus, and nonimmune fetal hydrops (NIFH) which, even if there are some etiological factors involved, causes hipoxic hypoxia in the fetus. Twelve fetuses were studied by way of 30 fetal blood samples obtained by ultrasound-guided cordocentesis between the 20th and 36th gestational week. Twenty-four blood samples in all where taken from the eight fetuses with Rh-isoimmunization. Six blood samples were obtained from the four fetuses with NIFH. The lymphocyte phenotypes studied by monoclonal antibodies and flow cytometry were the following: CD3, CD4, CD8, expression of T-lymphocyte subpopulations; BsIg, CD19, expression of B-lymphocyte subpopulations. We observed a near-normal maturation process in fetuses with Rh isoimmunization, whereas in fetuses with NIFH we observed inhibition and/or delayed expression of T-lymphocytes. An early and increased B-lymphocyte activation marked a cooperation between the two systems in the early gestational periods.

  8. A novel soluble supramolecular system for sustained rh-GH delivery.

    Science.gov (United States)

    Salmaso, Stefano; Bersani, Sara; Scomparin, Anna; Balasso, Anna; Brazzale, Chiara; Barattin, Michela; Caliceti, Paolo

    2014-11-28

    Methoxy-poly(ethylene glycol)s bearing a terminal cholanic moiety (mPEG(5kDa)-cholane, mPEG(10kDa)-cholane and mPEG(20kDa)-cholane) were physically combined with recombinant human growth hormone (rh-GH) to obtain supramolecular assemblies for sustained hormone delivery. The association constants (Ka) calculated by Scatchard analysis of size exclusion chromatography (SEC) data were in the order of 10(5)M(-1). The complete rh-GH association with mPEG(5kDa)-cholane, mPEG(10kDa)-cholane and mPEG(20kDa)-cholane was achieved with 7.5 ± 1.1, 3.9 ± 0.4 and 2.6 ± 0.4 w/w% rh-GH/mPEG-cholane, respectively. Isothermal titration calorimetry (ITC) yielded association constants similar to that calculated by SEC and showed that rh-GH has 21-25 binding sites for mPEG-cholane, regardless the polymer molecular weight. Dialysis studies showed that the mPEG-cholane association strongly delays the protein release; 80-90% of the associated rh-GH was released in 200 h. However, during the first 8h the protein formulations obtained with mPEG(10kDa)-cholane and mPEG(20kDa)-cholane showed a burst release of 8 and 28%, respectively. Circular dichroism (CD) analyses showed that the mPEG(5kDa)-cholane association does not alter the secondary structure of the protein. Furthermore, mPEG(5kDa)-cholane was found to enhance both the enzymatic and physical stability of rh-GH. In vivo pharmacokinetic and pharmacodynamic studies were performed by subcutaneous administration of rh-GH and rh-GH/mPEG(5kDa)-cholane to normal and hypophysectomised rats. The study showed that mPEG(5kDa)-cholane decreases the maximal concentration in the blood but prolongs the body exposure of the protein, which resulted in 55% bioavailability increase. Finally, rh-GH formulated with mPEG(5kDa)-cholane yielded prolonged weight increase of hypophysectomised rats as compared to rh-GH in buffer or formulated with mPEG(5kDa)-OH. After the second administration the weight of the animals treated with rh-GH formulated with m

  9. Phylogenetic positions of RH blood group-related genes in cyclostomes.

    Science.gov (United States)

    Suzuki, Akinori; Endo, Kouhei; Kitano, Takashi

    2014-06-10

    The RH gene family in vertebrates consists of four major genes (RH, RHAG, RHBG, and RHCG). They are thought to have emerged in the common ancestor of vertebrates after two rounds of whole genome duplication (2R-WGD). To analyze the detailed phylogenetic relationships within the RH gene family, we determined three types of cDNA sequence that belong to the RH gene family in lamprey (Lethenteron reissneri) and designated them as RHBG-like, RHCG-like1, and RHCG-like2. Phylogenetic analyses clearly showed that RHCG-like1 and RHCG-like2 genes, which were probably duplicated in the lamprey lineage, are orthologs of gnathostome RHCG. In contrast, the clear phylogenetic position of the RHBG-like gene could not be obtained. Probably some convergent events for cyclostome RHBG-like genes prevented the accurate identification of their phylogenetic positions. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Effect of GnRH and hCG on progesterone concentration and ovarian and luteal blood flow in diestrous mares.

    Science.gov (United States)

    Brito, L F C; Baldrighi, J M; Wolf, C A; Ginther, O J

    2017-01-01

    The objective of the present study was to investigate the effect of reproductive hormones (GnRH, hCG, LH and progesterone) on the regulation of corpus luteum (CL) and ovarian blood flow. Diestrous mares received a single treatment of saline, 100μg gonadorelin (GnRH), or 1500IU hCG 10days after ovulation. Plasma LH and progesterone concentrations, resistance index (RI) for ovarian artery blood-flow, and percentage of corpus luteum (CL) with color-Doppler signals of blood flow were determined immediately before treatment (hour 0) and at hours 0.25, 0.5, 1, 1.5, 2, 3, 4, 5, and 6. In the GnRH group, LH increased (Pblood-flow signals was greater at hour 0.5 in the GnRH group than in the saline group and was intermediate in the hCG group. The similarity among groups in progesterone concentration indicated that changes in progesterone were not involved in the GnRH and hCG stimulation of ovarian vascular perfusion. Effects of treatment might have been mediated through LH; however, since hCG biological activity is primarily LH-like, the differences in timing and degree of ovarian and luteal blood flow changes after GnRH or hCG administration in the present study suggest that GnRH might have a direct effect on ovarian blood vessels and vascular control.

  11. ABO/Rh Blood Groups and Risk of HIV Infection and Hepatitis B Among Blood Donors of Abidjan, Côte D’ivoire

    Science.gov (United States)

    Siransy, Liliane Kouabla; Nanga, Zizendorf Yves; Zaba, Flore Sandrine; Tufa, Nyasenu Yawo; Dasse, Sery Romuald

    2015-01-01

    Hepatitis B and HIV infection are two viral infections that represent real global public health problems. In order to improve their management, some hypotheses suggest that genetic predispositions like ABO and Rh blood groups would influence the occurrence of these diseases. The aim of the present study was to examine the association between ABO and Rhesus blood groups and the susceptibility to HIV infection and hepatitis B. We conducted a cross-sectional and analytical study in a population of voluntary blood donors in the Blood Transfusion Center of Abidjan. All blood donors who donated blood between January and June 2014 were tested for HBs antigen and anti-HIV antibodies (ELISA tests) and were ABO typed. The total number of examined blood donors during this period was 45,538, of which 0.32% and 8.07% were respectively infected with HIV and hepatitis B virus. O-group donors were more infected than non-O donors. Our study is an outline concerning the search for a link between ABO and Rh blood groups and hepatitis B and HIV infection. Further studies should be conducted to confirm the interaction between these two infections and contribute to the search for new therapeutic approaches. PMID:26495131

  12. ABO/Rh Blood Groups and Risk of HIV Infection and Hepatitis B Among Blood Donors of Abidjan, Côte D'ivoire.

    Science.gov (United States)

    Siransy, Liliane Kouabla; Nanga, Zizendorf Yves; Zaba, Flore Sandrine; Tufa, Nyasenu Yawo; Dasse, Sery Romuald

    2015-09-01

    Hepatitis B and HIV infection are two viral infections that represent real global public health problems. In order to improve their management, some hypotheses suggest that genetic predispositions like ABO and Rh blood groups would influence the occurrence of these diseases. The aim of the present study was to examine the association between ABO and Rhesus blood groups and the susceptibility to HIV infection and hepatitis B. We conducted a cross-sectional and analytical study in a population of voluntary blood donors in the Blood Transfusion Center of Abidjan. All blood donors who donated blood between January and June 2014 were tested for HBs antigen and anti-HIV antibodies (ELISA tests) and were ABO typed. The total number of examined blood donors during this period was 45,538, of which 0.32% and 8.07% were respectively infected with HIV and hepatitis B virus. O-group donors were more infected than non-O donors. Our study is an outline concerning the search for a link between ABO and Rh blood groups and hepatitis B and HIV infection. Further studies should be conducted to confirm the interaction between these two infections and contribute to the search for new therapeutic approaches.

  13. 南充市 Rh 阴性献血者血清学表型和不规则抗体调查%Investigation on serological phenotype and irregular antibody of Rh negative blood donors in Nanchong

    Institute of Scientific and Technical Information of China (English)

    杨松; 张伶; 邹韬; 杨兴明; 赵冰海; 陈科宇

    2015-01-01

    Objective To investigate the serological phenotype and irregular antibody of Rh negative blood do‐nors in Nanchong .Methods Blood group serology was used to reconfirm Rh negative、to screen and identify the sero‐logical phenotype and irregular antibody for Rh negative blood donors in primary detectionResults Among 433 speci‐mens ,a total of 402 cases(92 .8% ) were confirmed as Rh negative ,23 cases were D variants(5 .3% ) ,and the rest 8 cases were check errors(1 .9% );Irregular antibodies were found in 12 cases ,and 7 cases of anti‐D(1 .7% ) in recon‐firmed Rh negative blood donor ;The serological phenotype of Rh negative blood donor was given priority to dccee and dCcee ,followed by dCCee ,dccEe and dCcEe .Conclusion The serological phenotype of the Rh negative blood donors in our region Characterized by diversity ,there was irregular antibodies in Rh negative blood donors .%目的:调查南充市Rh阴性无偿献血者的血清学表型和不规则抗体情况。方法应用血型血清学方法对初检Rh阴性的献血者进行Rh阴性确认、血清学表型鉴定及不规则抗体筛选鉴定。结果在433份送检标本中共确认Rh阴性402份(92.8%),检出D变异体23份(5.3%),其余8份(1.9%)为检验科RhD检测错误;共检出不规则抗体12份,在Rh确认阴性献血者中检出7份抗‐D (1.7%);Rh阴性献血者的血清学表型分布结果主要以dccee和dCcee为主,其余为dCCee、dccEe和dCcEe。结论通过本次调查,掌握了南充市Rh阴性无偿献血者的血清学表型和不规则抗体的分布情况,保障了临床Rh阴性患者输血安全性、有效性和及时性。

  14. Magnetism in the system Ce1-xLaxRh3B2 (abstract)

    Science.gov (United States)

    Malik, S. K.; Umarji, A. M.; Shenoy, G. K.; Aldred, T. A.; Niarchos, D. G.

    1985-04-01

    The RRh3B2 (R=La to Gd) compounds form in the hexagonal CeCo3B2-type structure. The La containing compound in this series has been reported to be a superconductor while CeRh3B2 orders magnetically with Ce being in a nearly 3+ state. The magnetic ordering in CeRh3B2 is presumed to arise from the hybridization of nearly localized 4f electrons of Ce with sp conduction electrons or the 4d band of Rh or from the hybridization of the two. We have examined the magnetic behavior of the system Ce1-xLaxRh3B2 (x=0.0, 0.2, 0.4, 0.6, and 0.8). All these compounds (except LaRh3B2) also order magnetically. The Curie temperature decreases from about 115 K for pure Ce compound to 2.3 K for Ce0.2La0.8Rh3B2. The magnetic moments per formula unit remains nearly constant for 0Rh3B2 for x ranging from 0.0 to 0.8. The La-rich side is being investigated for the occurrence of superconductivity. Details will be published elsewhere.

  15. Frequency of ABO and Rh (D) Blood Groups and Hemoglobin Threshold among Pregnant Women in Family Guidance Association, Mekelle Model Clinic, North Ethiopia.

    OpenAIRE

    Megbaru Alemu; Guesh Abrha; Gessessew Bugssa; Kiros Tedla

    2014-01-01

    This study was aimed to determine the frequency of ABO and Rh blood group patterns and hemoglobin threshold among pregnant women screened at Family Guidance Association of Ethiopia, North Area Mekelle model clinic. Checklists were prepared to collect data from laboratory registration books of five years to undergo this retrospective study. Those registration books were reviewed for ABO blood group, Rh profiles and hemoglobin threshold of the pregnant women screened during the specified per...

  16. Ferromagnetic enhancement in the hexagonal Ce(Rh1-xIrx)3B2 system

    Science.gov (United States)

    Hsu, S. W.; Ku, H. C.

    1988-08-01

    Electrical resistivity, magnetization, and crystallographic measurements have been carried out for the pseudoternary system Ce(Rh1-xIrx)3B2. A single-phase hexagonal CeCo3B2-type structure persists from x=0.0 up to x0.95. The ferromagnetic Curie temperature Tc is enhanced from 115 K for CeRh3B2 to 125 K for Ce(Rh0.8Ir0.2)32, then decreases slowly to 118 K at x=0.5 before decreasing sharply to 69 K at x=0.67, even though the magnetic moment per Ce ion decreases monotonically with increasing Ir substitution. The Tc of 125 K in Ce(Rh0.8Ir0.2)3B2 is the highest magnetic-ordering temperature observed so far for all Ce-based systems with nonmagnetic constituents.

  17. The study of Allelic Frequency of ABO and Rh D Blood Group among the Banjara Population of Akola District, Maharashtra, India

    Directory of Open Access Journals (Sweden)

    Aravind Chavhan

    2012-12-01

    Full Text Available The distribution of ABO blood groups and Rh (D factor has been studied in the Banjara population. In the present study O, A, B, and AB blood group percentages of Banjaras of Akola district of Maharashtra are recorded as 27.64%, 22.91%, 37.45% and 12% respectively and the Rh negative incidences recorded as 02.55%. The allelic frequencies of O, A, B and AB groups in the combined data of same community found to be 0.5196, 0.2880, and 0.1924 respectively and Rh (D positive as 0.8405.

  18. Caracterización de donantes voluntarios de sangre por grupo sanguíneo A B O y Rh que asistieron a un banco de sangre de la ciudad de Tunja- Colombia./Characterization of voluntary blood donors for blood group ABO and Rh attending a blood bank in the city

    OpenAIRE

    2012-01-01

    Background: The frequency of ABO blood group and Rh factor is an important factor in relation to the needs of blood components in the population, identifying a routine in blood banks.Materials and methods: A retrospective, cross sectional, descriptive study was performed, period from January to March 2012 with data provided by the Foundation from Colombia Hematological volunteer blood donors who attended a fixed point of blood collection in the city of Tunja - Colombia. The variables analyzed...

  19. Peculiarities of Blood Group Distribution among Infants Born to Mothers with Negative Rh-Factor (Findings of 2014

    Directory of Open Access Journals (Sweden)

    Oksana G. Cherniukh

    2016-03-01

    Full Text Available Our works consider the investigation of possible manifestation of hyperbilirubinemia in infants against the ground of genetic incompatibilities of the fetus according to АВ0 system and Rh-factor (D concerning the maternal organism. From this point of view we deal with jaundice of mixed genesis against erythroblastosis domination as a primary antenatal factor of pathological process formation. The present study presents the results of distribution of the group and rhesus determinants (Rh D of infants born to mothers with negative Rh-factor in 2014. Analytical review and comparison with the previous investigations in this direction have been made. New trends of further work with the elements of chronobiological characteristics concerning possible signs of hemolytic diseases of newborns (HDN, neonatal isoerythrolysis, in Bukovyna region are outlined. The values of umbilical bilirubin concentration are taken as a biochemical criterion of HDN development which is the main diagnostic sign of pathological jaundice of newborns. The signs of HDN of various degree were observed in 24 infants out of 333 neonates born to mothers with negative Rh-factor during the period of 2014.

  20. [RH Factor and Clinical Transfusion Effectiveness for β-Thalassemia Children with Long-Term Blood Transfusion].

    Science.gov (United States)

    Wu, Chang-Lin; Wang, Xiao-Hua; He, Jian-An; Gu, Da-Yong; Dang, Xing-Tang; Zhu, Yi; Shao, Chao-Peng

    2015-12-01

    To investigate the irregular antibody production and its relationship with Rh factor genotypes and the loci of thalassemia gene mutations for the β-thalassemic children with long-term transfusion, so as provide experimental basis for clinical safe and effective transfusions for thalassemic children. The peripheral blood from 246 children with β-thalassemia was collected in our hospital; the extraction of genomic DNA and Rh factor (C/c, E/e) genotypes were assayed by PCR-SSP method, the irregular antibodies were screened and identified by serological method, the genotypes for thalassemia and gene mutations were analysed by PCR-RD method. The genotypes of Rh factors classified by PCR- SSP in the 246 cases of β-thalassemia children were as follws: Ce/Ce (143/246, 58.1%), CE/ce (59/246, 24%), cE/cE (14/24, 5.7%), Ce/ce (12/246, 4.9%); The positive rate of irregular antibody was 7.7% (19/246), including anti-E (7/19), anti-c (5/19), anti-C (2/19), anti-E and anti-c (2/19), anti-e (1/19), anti-D (2/19); Of the 19 cases with positive irregular antibody, the genotypings of Rh factor were: Ce/Ce (11/19), CE/ce (2/19), cE/cE (2/19), Ce/ce (2/19), cE/ce (2/19); the gene mutations location of thalassemia for 19 cases with positive irregular antibody: CD41-42M (13/19), CD71-72M (2/19), IVS-II-654M (3/19), -28M (1/19). The irregular antibody production for β-thalassemic children with long-term transfusion may have some relevance with Rh factor genotypes and thalassemia genetic mutations. This study possesses a certain significance for effective prevention of RBC alloimmune response of β-thalassemia children and improvement of efficacy and safety of clinical transfasion blood.

  1. Numerical Simulation of the Multiphase Flow in the Rheinsahl-Heraeus (RH) System

    Science.gov (United States)

    Geng, Dian-Qiao; Lei, Hong; He, Ji-Cheng

    2010-02-01

    Knowledge of gas-liquid multiphase flow behavior in the Rheinsahl-Heraeus (RH) system is of great significance to clarify the circulation flow rate, decarburization, and inclusion removal with a reliable description. Thus, based on the separate model of injecting gas behavior, a novel mathematical model of multiphase flow has been developed to give the distribution of gas holdup in the RH system. The numerical results show that the predicted circulation flow rates, the predicted flow velocities, and the predicted mixing times agree with the measured results in a water model and that the predicted tracer concentration curve agrees with the results obtained in an actual RH system. With a lower lifting gas flow rate, the rising gas bubbles are concentrated near the wall; with a higher lifting gas flow rate, gas bubbles can reach the center of the up-snorkel. A critical lifting gas flow rate is used to obtain the maximum circulation flow rate.

  2. Women's attitude towards prenatal screening for red blood cell antibodies, other than RhD

    Directory of Open Access Journals (Sweden)

    van der Schoot CE

    2008-11-01

    Full Text Available Abstract Background Since July 1998 all Dutch women (± 200,000/y are screened for red cell antibodies, other than anti-RhesusD (RhD in the first trimester of pregnancy, to facilitate timely treatment of pregnancies at risk for hemolytic disease of the fetus and newborn (HDFN. Evidence for benefits, consequences and costs of screening for non-RhD antibodies is still under discussion. The screening program was evaluated in a nation-wide study. As a part of this evaluation study we investigated, according to the sixth criterium of Wilson and Jüngner, the acceptance by pregnant women of the screening program for non-RhD antibodies. Methods Controlled longitudinal survey, including a prenatal and a postnatal measurement by structured questionnaires. Main outcome measures: information satisfaction, anxiety during the screening process (a.o. STAI state inventory and specific questionnaire modules, overall attitude on the screening program. Univariate analysis was followed by standard multivariate analysis to identify significant predictors of the outcome measures. Participants: 233 pregnant women, distributed over five groups, according to the screening result. Results Satisfaction about the provided information was moderate in all groups. All screen- positive groups desired more supportive information. Anxiety increased in screen- positives during the screening process, but decreased to basic levels postnatally. All groups showed a strongly positive balance between perceived utility and burden of the screening program, independent on test results or background characteristics. Conclusion Women highly accept the non-RhD antibody screening program. However, satisfaction about provided information is moderate. Oral and written information should be provided by obstetric care workers themselves, especially to screen-positive women.

  3. 贵州水族Rh血型分布%Rh blood group distribution in Guizhou Aquarium

    Institute of Scientific and Technical Information of China (English)

    钟江; 潘钦瑞; 杨元

    2010-01-01

    目的:了解贵州水族中Rh血型分布及基因频率,给水族Rh溶血症防治提供科学依据.方法:抽取贵州水族封闭群体、汉族对照组中的无关个体进行Rh血型鉴定和分析,推算Rh基因频率.结果:共筛查水族3190人,汉族对照4851人.发现水族Rh(D)阴性个体3名,汉族Rh(D)阴性个体16名;水族d基因频率0.0307、Rh(D)阴性率为0.09%,汉族(对照组)d基因频率0.0574、Rh阴性率为0.33%.结论:水族Rh阴性比例低于汉族.因此,贵州水族中Rh新生儿溶血症发生率可能不高.

  4. The investigation of infants with RhD-negative mothers: can we safely omit the umbilical cord blood direct antiglobulin test?

    Science.gov (United States)

    James, R M; McGuire, W; Smith, D P

    2011-07-01

    Historically, the investigation of a neonate at risk of Rhesus D antigen (RhD)-associated haemolytic disease has included a direct antiglobulin test on umbilical cord blood. However, the introduction of routine antenatal anti-RhD prophylaxis has led to a significant number of false positive results and recent studies suggest that a positive cord blood direct antiglobulin test is poorly predictive of subsequent hyperbilirubinaemia. The British Committee for Standards in Haematology guidelines now recommend that a direct antiglobulin test should no longer be performed routinely on umbilical cord blood in infants born to RhD-negative mothers. We review the recent changes in antenatal management of RhD-negative mothers and their impact on the neonatal presentation of RhD-associated haemolytic disease of the newborn that underpin this recommendation. We conclude that there is convincing evidence to support the guidelines. Finally, we consider how babies born to RhD-negative mothers should be investigated and consider alternative strategies to detect neonatal hyperbilirubinaemia.

  5. Human allogeneic AB0/Rh-identical umbilical cord blood cells in the treatment of juvenile patients with cerebral palsy.

    Science.gov (United States)

    Romanov, Yury A; Tarakanov, Oleg P; Radaev, Sergey M; Dugina, Tamara N; Ryaskina, Svetlana S; Darevskaya, Anna N; Morozova, Yana V; Khachatryan, William A; Lebedev, Konstantin E; Zotova, Nelli S; Burkova, Anna S; Sukhikh, Gennady T; Smirnov, Vladimir N

    2015-07-01

    The term "cerebral palsy" (CP) encompasses many syndromes that emerge from brain damage at early stages of ontogenesis and manifest as the inability to retain a normal body position or perform controlled movements. Existing methods of CP treatment, including various rehabilitation strategies and surgical and pharmacological interventions, are mostly palliative, and there is no specific therapy focused on restoring injured brain function. During a post-registration clinical investigation, the safety and efficacy of intravenous infusion of allogeneic human leukocyte antigen (HLA)-unmatched umbilical cord blood (UCB) cells were studied in 80 pediatric patients with cerebral palsy and associated neurological complications. Patients received up to 6 intravenous infusions of AB0/Rh-identical, red blood cell-depleted UCB cells at an average dose of 250 × 10(6) viable cells per infusion. Patients were followed for 3-36 months, and multiple cell infusions did not cause any adverse effects. In contrast, in most patients who received four or more UCB cell infusions, positive dynamics related to significant improvements in neurological status and/or cognitive functions were observed. The results confirm that multiple intravenous infusions of allogeneic AB0/Rh-identical UCB cells may be a safe and effective procedure and could be included in treatment and rehabilitation programs for juvenile patients with cerebral palsy. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

  6. [The relationship between rhGH and blood sugar on different ages of severe degree burned patients].

    Science.gov (United States)

    Chen, Jiong; Xia, Shi-chun; Xie, Bing; Tang, Zhi-jian; Su, Guo-liang; Shi, Jian-wu; Lu, Xue-mian

    2009-08-01

    Probe the effects of rhGH on severe degree burned patients' blood sugar in different age of years. Elected 210 patients hospitalized in the Third Affiliated Hospital of Wenzhou Medical College from January 2005 to December 2008, who were burned in 48 h, older than 18 years, ever had no diabetes and tumor history and placidly pull through shock stage. Among the patients there were 132 males and 78 females. The age was from 18 to 65 years old, average (40.7 +/- 7.2) years old. The extent of burn were form TBSA 25% to TBSA 86%, average TBSA (40.4 +/- 12.5)%. The depths of burn were from superficial second degree to third degree. All of the total divided into A (18 - 44 years old) and B (> 45 years old)groups. Each group had 105 patients. Two groups were randomly divided into A(1), A(2), A(0) and B(1), B(2), B(0) groups. Each group had 35 patients. The A(1) and B(1) groups were used 0.15 U/(kg.d) growth hormone (Somatropin, S19990021), A(2) and B(2) groups were used 0.2 U/(kg.d) growth hormone, A(0) and B(0) groups were used NS as control. Observed and analyzed the change of blood sugar and insulin amount used in 210 patients. Of all the patients in 6 groups, there were 190 patients finished the experimentation in four weeks. The insulin amount of A(1), A(2), A(0) groups used were (2123.3 +/- 152.3), (2885.6 +/- 148.5), (724.1 +/- 31.1) U, B(1), B(2), B(0) group were (2715.1 +/- 95.3), (3652.2 +/- 198.1), (801.8 +/- 22.2) U. The consequence showed that the number need insulin to control blood sugar in B group was more than A group, as well as using 0.2 U/(kg.d) does to 0.15 U/(kg.d) does, and using growth hormone to no using(P 0.05). The blood sugar's alteration has positive correlation with the age of years and the does of rhGH. As long as normative using rhGH it doesn't induce diabetes.

  7. How Is Rh Compatibility Diagnosed?

    Science.gov (United States)

    ... status, your doctor may do a test called amniocentesis. For this test, your doctor inserts a hollow ... whether the baby is Rh-positive. (Rarely, an amniocentesis can expose you to Rh-positive blood). Your ...

  8. An electro-hydraulic servo control system research for CFETR blanket RH

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Changqi [Hefei University of Technology, Hefei 230009, Anhui (China); Tang, Hongjun, E-mail: taurustang@126.com [Hefei University of Technology, Hefei 230009, Anhui (China); Qi, Songsong [Hefei University of Technology, Hefei 230009, Anhui (China); Cheng, Yong; Feng, Hansheng; Peng, Xuebing; Song, Yuntao [Institute of Plasma Physics Chinese Academy of Sciences, Hefei 230031, Anhui (China)

    2014-11-15

    Highlights: • We discussed the conceptual design of CFETR blanket RH maintenance system. • The mathematical model of electro-hydraulic servo system was calculated. • A fuzzy adaptive PD controller was designed based on control theory and experience. • The co-simulation models of the system were established with AMESim/Simulink. • The fuzzy adaptive PD algorithm was designed as the core strategy of the system. - Abstract: Based on the technical design requirements of China Fusion Engineering Test Reactor (CFETR) blanket remote handling (RH) maintenance, this paper focus on the control method of achieving high synchronization accuracy of electro-hydraulic servo system. Based on fuzzy control theory and practical experience, a fuzzy adaptive proportional-derivative (PD) controller was designed. Then a more precise co-simulation model was established with AMESim/Simulink. Through the analysis of simulation results, a fuzzy adaptive PD control algorithm was designed as the core strategy of electro-hydraulic servo control system.

  9. Is current serologic RhD typing of blood donors sufficient for avoiding immunization of recipients? (CME)

    DEFF Research Database (Denmark)

    Krog, Grethe Risum; Clausen, Frederik Banch; Berkowicz, Adela

    2011-01-01

    BACKGROUND: Avoiding immunization with clinically important antibodies is a primary objective in transfusion medicine. Therefore, it is central to identify the extent of D antigens that escape routine RhD typing of blood donors and to improve methodology if necessary. STUDY DESIGN AND METHODS: We......-specific primers. Lookback was performed on all recipients of RBCs from RHD+ donors. RESULTS: We found 13 RHD+ samples (0.26%). No variants or chimeras were found. Characterization of DNA revealed a novel DEL type (IVS2-2 A>G). In the lookback of the 136 transfusions with subsequent antibody follow-up, of which 13...... were from DEL donors, one recipient developed anti-D. However, in this case, a competing and more likely cause of immunization was the concurrent transfusion of D+ platelets. Eleven recipients were immunized with 13 antibodies different from anti-D, of which five were anti-K. CONCLUSION: In our...

  10. RhD-negative donor database and red blood cell 80 ℃ clinical application of cryopreservation%RhD阴性供者信息库与红细胞-80℃深低温保存的临床应用

    Institute of Scientific and Technical Information of China (English)

    杨毓明; 柯卫泽; 杨成睿; 刘艳芳

    2011-01-01

    目的 通过建立RhD阴性供者与红细胞-80 ℃深低温保存信息库,分析本地区RhD阴性血型分布状况,合理制备和保存RhD阴性成分血.方法 采集无偿献血者标本,用血型血清学方法 筛选RhD阴性供者,并进行RhD阴性鉴定确认和CcE表型分型,创建RhD阴性供者信息库.按计划制备并用深低温等技术保存RhD阴性成分血.结果 1998年10月~2010年6月共筛查无偿献血者384 334例,最后鉴定确认RhD阴性供者1 126(2.93‰)例.其中A型200例(32.63%)、B型153例(24.96%)、O型203例(33.12%)和AB型57例(9.30%).表型ccdee 369(60.20%)例,Ccdee 179(29.20%)例,CCdee 38(6.20%)例,ccdE 19(3.10%)例,CcdE 8(1.31%)例.10年向临床供应RhD阴性血液1 375单位.结论 建立RhD阴性供者信息库,为输注RhD阴性血的患者,特别是已产生同种抗体患者,提供RhD和CcEe表型相配合的供者血液,有效预防因Rh抗原同种免疫反应所致的各类溶血性输血反应,提高血液输注的疗效.%Objective To establish a information database of RhD negative and red cell 80℃ cryopreservation, analyze the dis tribution of RhD negative blood group in local area, then reasonably make and preserve componential blood of RhD negative. Methods By collecting the blood samples of volunteer blood donors, screen RhD negative donors with serological and identified for RhD-negative confirmation and CcE phenotype, create a RhD-negative donor database. Preparation according to plan and save with deep hypothermia and other technology components RhD negative blood. Results During October 1998 and June 2010,384 334 ca ses of voluntary blood donors were screened, the final and identified as RhD negative donors I 126(2.93 %0). Of which 200 cases of type A (32.63%),B type of 153 cases (24. 96%),O type 203 cases (33. 12%) and AB type 57 cases (9. 30%). Phenotype ccdee 369 cases (60.20%),Ccdee 179 cases (29.20%),CCdee 38 cases (6.20%),ccdE 19 cases (3. 10%) and CcdE 8 cases (1.31

  11. Frecuencias de grupos sanguíneos e incompatibilidades ABO y RhD, en La Paz, Baja California Sur, México Blood group ABO and RhD frequencies and incompatibilities in La Paz, Baja California Sur, Mexico

    Directory of Open Access Journals (Sweden)

    Lorenzo del Peón-Hidalgo

    2002-09-01

    Full Text Available Objetivo. Determinar las frecuencias génicas y de fenotipo, y predecir el riesgo de incompatibilidad y aloinmunización materna en la población de La Paz, Baja California Sur, México. Material y métodos. Estudio descriptivo en el que se evaluaron 1 809 disponentes sanguíneos altruistas que acudieron en 1998 al Hospital General de Zona, del Instituto Mexicano del Seguro Social en La Paz, Baja California Sur, México, tipificados por aglutinación en tubo. Las frecuencias génicas fueron estimadas asumiendo condiciones de equilibrio, y las incompatibilidades y riesgo de aloinmunización de acuerdo con la literatura. Los datos fueron evaluados estadísticamente mediante ji². Resultados. La población reveló las siguientes frecuencias en porcentaje: O, 58.49; A, 31.40; B, 8.40; AB, 1.71; RhD, 95.36; y RhD negativo, 4.64 y las frecuencias génicas fueron i, 0.7648; I A, 0.1821; I B, 0.0519; D, 0.7845; d, 0.2155, respectivamente. Las incompatibilidades en parejas y maternofetal fueron 0.3023 y 0.1685 para ABO, 0.0442 y 0.0364 para RhD y 0.0134 y 0.0061 para incompatibilidad doble, respectivamente. La probabilidad de aloinmunización materna fue estimada en 0.0309. Conclusiones. En La Paz, los grupos O y RhD son los más abundantes, aunque las frecuencias están entre las más bajas en México, contrario a lo ocurrido para A y RhD negativo. La probabilidad de aloinmunización materna y las incompatibilidades son también elevadas. Los grupos ancestrales blanco, negro e indio interactuaron en la zona noroeste del país, determinando al migrar a Baja California Sur un mestizaje probablemente similar al resto de la zona noroeste.Objective. To determine genic and phenotypic frequencies and predict the risk of incompatibility and maternal alloimmunization in the population of La Paz. Material and Methods. This descriptive study evaluated 1809 voluntary blood donors attending in 1998 the Hospital General de Zona of Instituto Mexicano del Seguro Social

  12. Luteal blood flow in patients undergoing GnRH agonist long protocol

    Directory of Open Access Journals (Sweden)

    Takasaki Akihisa

    2011-01-01

    Full Text Available Abstract Background Blood flow in the corpus luteum (CL is closely related to luteal function. It is unclear how luteal blood flow is regulated. Standardized ovarian-stimulation protocol with a gonadotropin-releasing hormone agonist (GnRHa long protocol causes luteal phase defect because it drastically suppresses serum LH levels. Examining luteal blood flow in the patient undergoing GnRHa long protocol may be useful to know whether luteal blood flow is regulated by LH. Methods Twenty-four infertile women undergoing GnRHa long protocol were divided into 3 groups dependent on luteal supports; 9 women were given ethinylestradiol plus norgestrel (Planovar orally throughout the luteal phase (control group; 8 women were given HCG 2,000 IU on days 2 and 4 day after ovulation induction in addition to Planovar (HCG group; 7 women were given vitamin E (600 mg/day orally throughout the luteal phase in addition to Planovar (vitamin E group. Blood flow impedance was measured in each CL during the mid-luteal phase by transvaginal color-pulsed-Doppler-ultrasonography and was expressed as a CL-resistance index (CL-RI. Results Serum LH levels were remarkably suppressed in all the groups. CL-RI in the control group was more than the cutoff value (0.51, and only 2 out of 9 women had CL-RI values Conclusion Patients undergoing GnRHa long protocol had high luteal blood flow impedance with very low serum LH levels. HCG administration improved luteal blood flow impedance. This suggests that luteal blood flow is regulated by LH.

  13. Development of a Transnasal Delivery System for Recombinant Human Growth Hormone (rhGH): Effects of the Concentration and Molecular Weight of Poly-L-arginine on the Nasal Absorption of rhGH in Rats.

    Science.gov (United States)

    Kawashima, Ryo; Uchida, Masaki; Yamaki, Tsutomu; Ohtake, Kazuo; Hatanaka, Tomomi; Uchida, Hiroyuki; Ueda, Hideo; Kobayashi, Jun; Morimoto, Yasunori; Natsume, Hideshi

    2016-01-01

    A novel system for delivering recombinant human growth hormone (rhGH) that is noninvasive and has a simple method of administration is strongly desired to improve the compliance of children. The aim of this study was to investigate the potential for the intranasal (i.n.) co-administration of rhGH with poly-L-arginine (PLA) as a novel delivery system by evaluating the effects of the concentration and molecular weight of PLA on the nasal absorption of rhGH. The influence of the formation of insoluble aggregates and a soluble complex in the dosage formulation on nasal rhGH absorption was also evaluated by size-exclusion chromatography and ultrafiltration. PLA enhanced the nasal absorption of rhGH at each concentration and molecular weight examined. Nasal rhGH absorption increased dramatically when the PLA concentration was 1.0 % (w/v) due to the improved solubility of rhGH in the formulation. A delay in rhGH absorption was observed when the molecular weight of PLA was increased. This appeared to be because the increase in molecular weight caused the formation of a soluble complex. It seems that the PLA concentration affects the absorption-enhancing effect on rhGH, while the molecular weight of PLA affects the time when the maximum plasma rhGH concentration was reached (Tmax) of rhGH after i.n. administration, mainly because of the interactions among rhGH, PLA, and additives. Therefore, the transnasal rhGH delivery system using PLA is considered to be a promising alternative to subcutaneous (s.c.) injection if these interactions are sufficiently controlled.

  14. Identification and screening the mimic epitopes of human Rh(D)blood type antigens%人Rh(D)血型抗原模拟表位的筛选和鉴定

    Institute of Scientific and Technical Information of China (English)

    卞茂红; 沈际佳; 刘淼; 许伟; 杨鹏; 刘淑均; 钟涛

    2008-01-01

    Objective To screen the mimic epitopes of Rh(D)blood group antigens and identify their immunity from phage display peptide library.Methods A twelve mer phage peptide library was biopanned with anti-Rh(D)monoclonal antibody immobilized on plastic surface.After three round panning,thirty-five clones were randomly selected and positive clones were identified by ELISA and cross-reaction,followed by antibody competition inhibition assay and DNA sequencing to obtain the mimic epitopes of Rh (D)blood type antigens.The target phage clones were characterized and the antigenicity was analyzed by Western blot.Results After the third round screening,phages were enriched,and eleven positive clones were obtained.According to sequencing and competition inhibition analysis,the same"-WP-Q-"structure existed in seven of the eleven clones,and they had more than 40%inhibition ratio.The other clones had no same characteristics with low inhibition ratio possibly due to non-specific binding.Western blot analysis indicated that these phage clones could be specifically recognized by the anti-Rh(D)serum and they shared the same antigenicity of Rh(D)protein.Conclusions Rh(D)mimotope of"-WP-Q-"structure is successfully obtained by phage peptide library screening with anti-Rh(D)monoelonal antibody.The results lay the foundation for further exploration of pathogenesis and vaccine development of Rh(D)hemolytic diseases of newborn.%目的 从噬菌体随机肽库中筛选人Rh(D)血型抗原模拟表位,并对其免疫学活性进行鉴定.方法 以抗Rh(D)单克隆抗体作为固相筛选分子,对噬菌体随机十二肽库进行3轮"吸附-洗脱-扩增"筛选过程,随机挑选35个克隆,经噬菌体ELISA和交叉反应试验,确定阳性克隆,再对这些克隆进行DNA序列测定和抗体竞争抑制试验,以获取人Rh(D)血型抗原模拟表位.然后采用蛋白质免疫印迹法(Western blot)对所获噬菌体进行鉴定和抗原性分析.结果 经过3轮淘洗

  15. Stable solid-phase Rh antigen.

    Science.gov (United States)

    Yared, M A; Moise, K J; Rodkey, L S

    1997-12-01

    Numerous investigators have attempted to isolate the Rh antigens in a stable, immunologically reactive form since the discovery of the Rh system over 56 years ago. We report here a successful and reproducible approach to solubilizing and adsorbing the human Rh antigen(s) to a solid-phase matrix in an antigenically active form. Similar results were obtained with rabbit A/D/F red blood cell antigens. The antigen preparation was made by dissolution of the red blood cell membrane lipid followed by fragmentation of the residual cytoskeleton in an EDTA solution at low ionic strength. The antigenic activity of the soluble preparations was labile in standard buffers but was stable in zwitterionic buffers for extended periods of time. Further studies showed that the antigenic activity of these preparations was enhanced, as was their affinity for plastic surfaces, in the presence of acidic zwitterionic buffers. Adherence to plastic surfaces at low pH maintained antigenic reactivity and specificity for antibody was retained. The data show that this approach yields a stable form of antigenically active human Rh D antigen that could be used in a red blood cell-free assay for quantitative analysis of Rh D antibody and for Rh D antibody immunoadsorption and purification.

  16. A PfRH5-Based Vaccine Is Efficacious against Heterologous Strain Blood-Stage Plasmodium falciparum Infection in Aotus Monkeys

    Science.gov (United States)

    Douglas, Alexander D.; Baldeviano, G. Christian; Lucas, Carmen M.; Lugo-Roman, Luis A.; Crosnier, Cécile; Bartholdson, S. Josefin; Diouf, Ababacar; Miura, Kazutoyo; Lambert, Lynn E.; Ventocilla, Julio A.; Leiva, Karina P.; Milne, Kathryn H.; Illingworth, Joseph J.; Spencer, Alexandra J.; Hjerrild, Kathryn A.; Alanine, Daniel G.W.; Turner, Alison V.; Moorhead, Jeromy T.; Edgel, Kimberly A.; Wu, Yimin; Long, Carole A.; Wright, Gavin J.; Lescano, Andrés G.; Draper, Simon J.

    2015-01-01

    Summary Antigenic diversity has posed a critical barrier to vaccine development against the pathogenic blood-stage infection of the human malaria parasite Plasmodium falciparum. To date, only strain-specific protection has been reported by trials of such vaccines in nonhuman primates. We recently showed that P. falciparum reticulocyte binding protein homolog 5 (PfRH5), a merozoite adhesin required for erythrocyte invasion, is highly susceptible to vaccine-inducible strain-transcending parasite-neutralizing antibody. In vivo efficacy of PfRH5-based vaccines has not previously been evaluated. Here, we demonstrate that PfRH5-based vaccines can protect Aotus monkeys against a virulent vaccine-heterologous P. falciparum challenge and show that such protection can be achieved by a human-compatible vaccine formulation. Protection was associated with anti-PfRH5 antibody concentration and in vitro parasite-neutralizing activity, supporting the use of this in vitro assay to predict the in vivo efficacy of future vaccine candidates. These data suggest that PfRH5-based vaccines have potential to achieve strain-transcending efficacy in humans. PMID:25590760

  17. Abo and Rh Blood Groups Distribution in Hemophilia and Anti Hiv Positive Individuals

    Directory of Open Access Journals (Sweden)

    D.D. FARHUD

    1987-07-01

    Full Text Available A group of Iranian patients suffering from Factor VIII deficiency (Hemophilia A and treated with contaminated coagulation factor (imported, became seropositive as determined by ELISA method. Sixty of these individuals, which were available, were studied for ABO distribution. The B blood group in anti HIV pos. individuals (13.33% shows a significant decrease in comparison with the total (1504 of factor VIII hemophilia (21.87%. Statistical analysis of ABO distribution in anti HIV Pos. compared with hemophilia A and the control group showed x2 values of 6.86(0.10 > p>0.05 and 10.21(0.02> P >0.01 respectively.

  18. Molecular definition of red cell Rh haplotypes by tightly linked SphI RFLPs.

    Science.gov (United States)

    Huang, C H; Reid, M E; Chen, Y; Coghlan, G; Okubo, Y

    1996-01-01

    The Rh blood group system of human red cells contains five major antigens D, C/c, and E/e (the latter four designated "non-D") that are specified by eight gene complexes known as Rh haplotypes. In this paper, we report on the mapping of RH locus and identification of a set of SphI RFLPs that are tightly linked with the Rh structural genes. Using exon-specific probes, we have localized the SphI cleavage sites resulting in these DNA markers and derived a comprehensive map for the RH locus. It was found that the SphI fragments encompassing exons 4-7 of the Rh genes occur in four banding patterns or frameworks that correspond to the distribution and segregation of the common Rh haplotypes. This linkage disequilibrium allowed a genotype-phenotype correlation and direct determination of Rh zygosity related to the Rh-positive or Rh-negative status (D/D, D/d, and d/d). Studies on the occurrence of SphI RFLPs in a number of rare Rh variants indicated that Rh phenotypic diversity has taken place on different haplotype backgrounds and has arisen by diverse genetic mechanisms. The molecular definition of Rh haplotypes by SphI RFLP frameworks should provide a useful procedure for genetic counseling and prenatal assessment of Rh alloimmunization.

  19. 呼和浩特地区 RhD 阴性献血者表型库的建立及应用%Establishment and application of the phenotype database of negative RhD blood donors in Hohhot

    Institute of Scientific and Technical Information of China (English)

    尚锦青; 贾雯婷; 苏仁娜; 崔丽敏

    2015-01-01

    Objective To understand the phenotype distribution of negative RhD blood donors in local region ,and to establish negative RhD phenotype database .Methods A total of 554 specimens screened firstly as negative RhD were confirmed by indirect antiglobulin test .Repeat donors were excluded ,the remaining specimens were 366 cases ,which were analyzed the phenotype of Rh blood .Results Distribution characteristics of negative RhD phenotype was ccdee (56 .28% ) > Ccdee (29 .51% ) > ccdEe (7 .38% )> CcdEe (3 .55% )> CCdee (3 .01% )> ccdEE (0 .27% ) ,there were no CcdEE ,CCdEe ,CCdEE detected .Conclusion The establishment of Negative RhD phenotype database is help for providing matching blood for persons with negative RhD blood or same antibody blood ,and meeting the emergency blood usage ,it′s important for scientific management and reasonable application of negative RhD blood ,and accumulate data for then negative RhD blood of the local area ,especially for Mongolian .%目的:了解该地区 RhD 阴性献血者表型分布,建立 RhD 阴性表型数据库。方法 RhD 初筛阴性的554份标本采用间接抗球蛋白试验确认。排除重复献血者后,剩余标本为366份,对其进行 Rh 血型表型分析。结果 RhD 阴性表型分布特征为ccdee(56.28%)> Ccdee (29.51%)> ccdEe (7.38%)> CcdEe (3.55%)> CCdee (3.01%)> ccdEE (0.27%),未检出 CcdEE 、CCdEe 、CCdEE 型。结论建立 RhD 阴性表型数据库,有利于为 RhD 阴性及含有 Rh 同种抗体的患者提供相匹配血液,满足其急救用血,且对科学管理 RhD 阴性血源和合理运用 RhD 阴性血液有重要意义,为该地区尤其蒙古族 RhD 阴性血型积累了资料。

  20. Safety of autologous blood component transfusion during cesarean section in patients with Rh(D)-negative blood group%Rh(D)阴性血型病人剖宫产术中成分式自体输血的安全性

    Institute of Scientific and Technical Information of China (English)

    周春波; 叶松; 严海雅; 张檀; 黄巧波; 陈俊妍; 孙志强

    2011-01-01

    Objective To investigate the safety of autologous blood component transfusion during cesarean section in patients with Rh (D)-negative blood group.Methods Thirty ASA Ⅰ or Ⅱ patients of Rh (D)-negative blood group, aged 20-35 yr, weighing 50-80 kg, undergoing elective cesarean section, were enrolled in this study.After lactated Ringer' s solution 7 ml/kg was infused, blood was obtained from radial artery at a rate of 60-80ml/min, and blood volume was maintained by simultaneous infusion of 6% hydroxyethyl starch 130/0.4 at the same rate. The collected blood was subjected to two cycles of autologous blood component separation. Blood collecting during each cycle was stopped 15 s after red blood cells were separated. The autologous blood was infused when the blood loss≥20% of blood volume. The autologous blood was infused after suture of the uterus when the blood loss 0.05).脐动脉血pH值、BE和乳酸浓度均在正常范围内.胎儿娩出后1、5 min时Apgar评分分别为(9.0±0.8)、(9.2±0.8)分;术中出血量(405±28)ml,所有病人未输注异体血.结论 Rh(D)阴性血型病人剖宫产术中成分式自体输血的安全性良好.

  1. Molecular definition of red cell Rh haplotypes by tightly linked SphI RFLPs.

    OpenAIRE

    Huang, C. H.; Reid, M.E.; Chen, Y.; Coghlan, G.; Okubo, Y

    1996-01-01

    The Rh blood group system of human red cells contains five major antigens D, C/c, and E/e (the latter four designated "non-D") that are specified by eight gene complexes known as Rh haplotypes. In this paper, we report on the mapping of RH locus and identification of a set of SphI RFLPs that are tightly linked with the Rh structural genes. Using exon-specific probes, we have localized the SphI cleavage sites resulting in these DNA markers and derived a comprehensive map for the RH locus. It w...

  2. Frequencies of blood groups, ABO and Rh D incompatibility in post-delivery women and their liveborn

    OpenAIRE

    2007-01-01

    OBJETIVO: Determinar as freqüências fenotípicas e predizer o risco de incompatibilidade e aloimunização materna RhD na população da Zona Oeste de São Paulo, Brasil. MÉTODOS: Estudo descritivo no qual avaliamos 2372 puérperas e seus recém-nascidos vivos, no período de um ano, tipificadas para os sistemas ABO e RhD por meio de teste de aglutinação em tubo. RESULTADOS: O estudo mostrou os seguintes percentuais: grupo sangüíneo O, 50,67%; A, 32,17%; B, 13,45%; AB, 3,71%; RhD(+), 90,34% e RhD(-), ...

  3. [Gene frequencies and heterozygosity of the AB0 and RH blood group alleles in the populations of two cities of the Donetsk region, Ukraine].

    Science.gov (United States)

    Mukhin, V N; Chinakh, D G; Avdeev, A V; Kuleba, V V; Afanas'ev, M V

    2003-04-01

    The frequencies of the AB0 and RH blood group alleles and heterozygosity indices were determined for the populations of two large industrial cities of Gorlovka and Mariupol. In the population of Gorlovka the gene frequencies were as follows: AB0*0 = 0.576, AB0*A = 0.266, AB0*B = 0.158, and RH*D = 0.592, in Mariupol the frequencies were AB0*0 = 0.584, AB0*A = 0.265, AB0*B = 0.151, and RH*D = 0.604. In Gorlovka the heterozygosity indices in respect to the AB0 and RH alleles were 0.572 and 0.483, respectively; in Mariupol, 0.566 and 0.478, respectively. There were no statistically significant differences between the two populations in respect to the genetic markers analyzed. However, the heterozygosity values obtained were more similar to the corresponding estimates for some populations of Russia, than for the total population of the Ukraine.

  4. Freqüências dos grupos sangüíneos e incompatibilidades ABO e RhD em puérperas e seus recém-nascidos Frequencies of blood groups, ABO and Rh D incompatibility in post-delivery women and their liveborn

    Directory of Open Access Journals (Sweden)

    Eduardo Baiochi

    2007-02-01

    Full Text Available OBJETIVO: Determinar as freqüências fenotípicas e predizer o risco de incompatibilidade e aloimunização materna RhD na população da Zona Oeste de São Paulo, Brasil. MÉTODOS: Estudo descritivo no qual avaliamos 2372 puérperas e seus recém-nascidos vivos, no período de um ano, tipificadas para os sistemas ABO e RhD por meio de teste de aglutinação em tubo. RESULTADOS: O estudo mostrou os seguintes percentuais: grupo sangüíneo O, 50,67%; A, 32,17%; B, 13,45%; AB, 3,71%; RhD(+, 90,34% e RhD(-, 9,66%. A ocorrência de incompatibilidade materno-fetal foi de 18,4% para o sistema ABO e de 7% para o RhD. CONCLUSÃO: O contingente da população Rh negativa com alto risco para aloimunização RhD foi estimado em 82%, denotando a importância da profilaxia da aloimunização RhD.OBJECTIVE: This study aimed to assess the frequency of different blood phenotypes and to predict the risk of Rh D alloimmunization and maternal-fetal incompatibility in a Brazilian population living in the West zone of the city of São Paulo - Brazil. METHODS: This descriptive study evaluated 2,372 post-delivery women and their liveborn during one year. Blood types were analyzed by means of tube agglutination tests. RESULTS: The blood type frequencies were: 50.67 O, 32.17 A, 13.45 B, 3.75 AB, 90.34 Rh D(+ and 9.66 Rh D(-. ABO maternal-fetal incompatibility was detected in 18.4% and Rh D incompatibility in 7%. CONCLUSION: The fraction of Rh D(- population at high risk for Rh D alloimmunization was 82%, emphasizing the importance of Rh D alloimmunization profilaxis.

  5. Application of aluminum-supported Pd, Rh, and Rh-Pd nanoparticles in supercritical carbon dioxide system for hydrodebromination of polybrominated diphenyl ethers.

    Science.gov (United States)

    Wu, Ben-Zen; Sun, Yu-Jie; Chen, Yan-Hua; Yak, Hwa Kwang; Yu, Jya-Jyun; Liao, Weisheng; Chiu, KongHwa; Peng, Shie-Ming

    2016-08-01

    Al-powder-supported Pd, Rh, and Rh-Pd catalysts were synthesized through a spontaneous redox reaction in aqueous solutions. These catalysts hydrodebrominated 4- and 4,4'-bromodiphenyl ethers in supercritical carbon dioxide at 200 atm CO2 containing 10 atm H2 and 80 °C in 1 h. Diphenyl ether was the major product of Pd/Al. Rh/Al and Rh-Pd/Al further hydrogenated two benzene rings of diphenyl ether to form dicyclohexyl ether. The hydrogenolysis of CO bonds on diphenyl ether over Rh/Al and Rh-Pd/Al was observed to generate cyclohexanol and cyclohexane (Rh-Pd/Al among three catalysts is suggested to be used for ex situ degradation of polybrominated diphenyl ethers in supercritical carbon dioxide. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Liposome-based delivery systems for ginsenoside Rh2: in vitro and in vivo comparisons

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Linqiang [China Pharmaceutical University, Department of Pharmaceutics, State Key Laboratory of Natural Medicines (China); Yu, Hua [University of Macao, Institute of Chinese Medical Sciences (China); Yin, Shaoping; Zhang, Ruixia; Zhou, Yudan; Li, Juan, E-mail: lijuancpu@163.com [China Pharmaceutical University, Department of Pharmaceutics, State Key Laboratory of Natural Medicines (China)

    2015-10-15

    The Ginsenoside Rh2 (Rh2) has been shown to possess anti-cancer properties both in vitro and in vivo. However, the poor bioavailability and fast plasma elimination limit the further clinical applications of Rh2 for cancer treatments. In the present study, three types of Rh2-loaded liposomes including Rh2-loaded normal liposome (Rh2-LP), Rh2-loaded cationic liposome (Rh2-CLP), and Rh2-loaded Methoxy poly(ethylene glycol)-poly(lactide) (mPEG-PLA) liposome (Rh2-PLP) have been optimized and prepared with mean particle size of 80–125 nm. Compared to Rh2-LP, surface modifications with mPEG or octadecylamine significantly improve the physicochemical and biological properties both in vitro and in vivo. Moreover, PLP presented better tumor accumulation of the fluorescent cyanine dye, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) in HepG2-xenografted nude mice than CLP (1.3-fold) or LP (1.6-fold) and prolong the resident time of DiR in tumor and organs (more than 24 h). The in vivo anti-cancer efficacy assessments indicate that Rh2-PLP presents the most activity on suppressing tumor growth in HepG2-xenografted mice than Rh2-LP and Rh2-CLP and without any significant toxicity. Our results indicate that mPEG-PLA modified liposome should be a potential and promising strategy to enhance the therapeutic index for anti-cancer agents.

  7. Liposome-based delivery systems for ginsenoside Rh2: in vitro and in vivo comparisons

    Science.gov (United States)

    Xu, Linqiang; Yu, Hua; Yin, Shaoping; Zhang, Ruixia; Zhou, Yudan; Li, Juan

    2015-10-01

    The Ginsenoside Rh2 (Rh2) has been shown to possess anti-cancer properties both in vitro and in vivo. However, the poor bioavailability and fast plasma elimination limit the further clinical applications of Rh2 for cancer treatments. In the present study, three types of Rh2-loaded liposomes including Rh2-loaded normal liposome (Rh2-LP), Rh2-loaded cationic liposome (Rh2-CLP), and Rh2-loaded Methoxy poly(ethylene glycol)-poly(lactide) (mPEG-PLA) liposome (Rh2-PLP) have been optimized and prepared with mean particle size of 80-125 nm. Compared to Rh2-LP, surface modifications with mPEG or octadecylamine significantly improve the physicochemical and biological properties both in vitro and in vivo. Moreover, PLP presented better tumor accumulation of the fluorescent cyanine dye, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) in HepG2-xenografted nude mice than CLP (1.3-fold) or LP (1.6-fold) and prolong the resident time of DiR in tumor and organs (more than 24 h). The in vivo anti-cancer efficacy assessments indicate that Rh2-PLP presents the most activity on suppressing tumor growth in HepG2-xenografted mice than Rh2-LP and Rh2-CLP and without any significant toxicity. Our results indicate that mPEG-PLA modified liposome should be a potential and promising strategy to enhance the therapeutic index for anti-cancer agents.

  8. Endovascular blood flow measurement system

    Science.gov (United States)

    Khe, A. K.; Cherevko, A. A.; Chupakhin, A. P.; Krivoshapkin, A. L.; Orlov, K. Yu

    2016-06-01

    In this paper an endovascular measurement system used for intraoperative cerebral blood flow monitoring is described. The system is based on a Volcano ComboMap Pressure and Flow System extended with analogue-to-digital converter and PC laptop. A series of measurements performed in patients with cerebrovascular pathologies allows us to introduce “velocity-pressure” and “flow rate-energy flow rate” diagrams as important characteristics of the blood flow. The measurement system presented here can be used as an additional instrument in neurosurgery for assessment and monitoring of the operation procedure. Clinical data obtained with the system are used for construction of mathematical models and patient-specific simulations. The monitoring of the blood flow parameters during endovascular interventions was approved by the Ethics Committee at the Meshalkin Novosibirsk Research Institute of Circulation Pathology and included in certain surgical protocols for pre-, intra- and postoperative examinations.

  9. Alteration of RH gene structure and expression in human dCCee and DCW-red blood cells: phenotypic homozygosity versus genotypic heterozygosity.

    Science.gov (United States)

    Huang, C H

    1996-09-15

    This report describes a comparative study on the dCCee and DCW-red blood cells devoid of RhD and CcEe antigens, respectively. Southern blots showed that the two variants carried opposite deletions in the D and non-D (CcEe) genes. Rh haplotyping and exon polymerase chain reaction (PCR) assay indicated that the deletions did not extend beyond the 5' region upstream from exon 1 or the 3' region downstream from exon 10 of the respective genes. This was confirmed by finding intact promoters and 3' untranslated regions in both D and non-D genes in each variant. Reverse transcriptase-PCR and cDNA sequencing showed the expression of two transcripts in each cell type. In dCCee cells, one transcript was the regular Ce form and the other occurred as a D-Ce-D hybrid whose Ce sequence spanned exons 2 through 9. In DCW-cells, the two transcripts were derived from reversely arranged hybrid genes, ie, the CW-D gene was formed by fusion of CW exon 1 with D exons 2 through 10, whereas the reverse product was formed by fusion of D exons 1 through 9 with non-D exon 10. These results indicated that DNA deletion and recombination had occurred in either cis or trans configuration and involved both RH loci in the dCCee or DCW-genome. Identification of such compound alterations correlates the genotypes with phenotypes and explains the lost Rh antigenic expression. A reinvestigation of gene organization also led to the reassignment of several 5' and 3' splice sites. Together, this study not only shows the complexity of Rh phenotypic diversity, but also points to the importance of concurrent analysis of genomic structure and transcript expression in deciphering the underlying genetic mechanisms.

  10. Improvements in start system of ETE-RH/FP fans; Melhorias no sistema de partida dos ventiladores da ETE-RH/FP

    Energy Technology Data Exchange (ETDEWEB)

    Martinez, Flavio Costa; Sinzato, Frederico Takashi Di Tanno; Assis, Marcio Souza de [ArcelorMittal Tubarao, ES (Brazil). Dept. de Producao de Gusa e Energia

    2009-11-01

    The present technical contribution presents the results achieved with the improvement of start control of Effluent Treatment Station of RH furnace and Ladle furnace (ETE-RH/FP) fans. This work started from the necessity to find a solution to decrease the constant mechanical breaks of fans on the station, where it has been used two fans per cell in the tower, which means that when one is working, the other in the same cell is turned off. The second fan turns the other way round, and when the same is turned on it causes a 'bang' strong enough to break its elements, such as braces and gears. With the use of soft start, the same, besides to start the motor of the fan in a soft way, still does that the fan stop turning the other way round before the start, preventing the 'bangs' in the equipment. Another important item considered in the solution was the harmonic generation. The soft start generate few harmonic, if compared to a frequency inverter. The introduction of this new start system of fans of station, besides the reduction of costs of the repair maintenance, brought an important electrical energy saving, being aligned with the new projects of the company. (author)

  11. Sedimentation and contamination patterns of dike systems along the Rhône River (France)

    Science.gov (United States)

    Seignemartin, Gabrielle; Tena, Alvaro; Piégay, Hervé; Roux, Gwenaelle; Winiarski, Thierry

    2017-04-01

    Humans have historically modified the Rhône River, especially in the last centuries. In the 19th century, the river was systematically embanked for flood protection purposes, and works continued along the 20th century with dike system engineering work for navigation. The Rhône was canalised and its historical course by-passed by a series of hydroelectric dams. Besides, industrial activity polluted the river. For example, high levels of PCB's were attributed to the inputs of the heavily industrialized zone downstream from Lyon. During floods, these contaminants, associated with the suspended sediment, were trapped by the engineering works and the floodplain. Currently, a master plan to reactivate the river dynamics in the alluvial margins by removing the groyne-fields and dikes in the by-passed sections is being implemented. Within this context, this work aims to assess historical dynamics of sediment and associated contaminants in the floodplain (e.g. trace metal elements), notably in the dike system, in order to evaluate the contamination risk related to bank protection removal. With this objective, a transversal methodology has been applied coupling GIS diachronic analysis (old maps, bathymetric data, Orthophotos, LIDAR, etc.) to understand the historical floodplain evolution, sediment survey to obtain sediment thickness (metal rod and Ground Penetrating Radar), and sediment sampling (manual auger and core sampling) to obtain the metal element concentrations (X-Ray Fluorescence and Inductively Coupled Plasma Mass Spectrometry). By this way, metal element patterns were defined and used as contamination tracing indicators to apprehend the contamination history but also as geochemical background indicators to define the sediment source influence. We found that sediment temporal patterns are directly related with the by-pass construction year. Spatially, fine sediment deposition predominates in the dike systems, being lower in the floodplain already disconnected in

  12. Investigation of the distribution of Rh (D) negative group among unpaid blood donors in Baoji region%宝鸡地区无偿献血人群Rh(D)阴性血型分布调查

    Institute of Scientific and Technical Information of China (English)

    王建康; 虢小丽; 刘娟娟; 颜娟

    2016-01-01

    Objective To investigate the distribution of Rh (D) negative group among unpaid blood donors in Baoji region, to further improve the database of rare blood donors, and to ensure the supply of Rh(D) negative blood for clinical purposes. Methods From 2005 to 2014, Rh(D) negative blood group was selected from 377 485 unpaid blood donors and the initial screening negative samples were confirmed and analyzed for the distribution of Rh (D) donors. Results A total of 1 074 cases (0.29%) of Rh(D) negative were confirmed from 377 485 unpaid blood participants, with B type (31.01%)>O type (30.07%)>A type (28.03%)>AB type (10.89%), and the differences of differ-ent blood types were statistically significant (P0.05. Most Rh(D) negative unpaid blood donors were aged 18~42 years, and there was no statistically significant difference among different ages (P>0.05). Phenotype distribution characteristics were as follows: ccdee (52.05%), Ccdee (26.81%), ccdEe (11.45%), CCdee (5.12%), CcdEe (3.35%), CcdEE (0.47%), ccdEE (0.37%), CCdEe (0.19%), CCdEE (0.19%). Rh(D) negative phenotype and ABO blood group had no correla-tion. Conclusion Rh(D) negative detection rate in Baoji region of unpaid blood donation population is 0.29%. Ccdee and Ccdee are two main phenotypes in the selected negative blood types, while AB Rh negative accounts for less.%目的:了解宝鸡地区无偿献血人群Rh(D)阴性血型分布情况,进一步完善稀有血型献血者数据库,保障Rh(D)阴性血液的临床供应。方法对宝鸡地区2005-2014年377485名无偿献血者进行Rh(D)阴性血型筛选,初筛阴性标本进行确认并分析Rh(D)献血者分布情况。结果377485名无偿献血者中最终确认Rh(D)阴性1074例,占0.29%,其中B型(31.01%)>O型(30.07%)>A型(28.03%)>AB型(10.89%),差异有统计学意义(P0.05);年龄以18~42岁为主,差异无统计学意义(P>0.05);表现型分布特征为:ccdee (52.05%)>Ccdee (26.81%)>ccdEe (11.45%)>CCdee (5.12%)>CcdEe (3

  13. RHD gene polymorphism among RhD-negative Han Chinese

    Institute of Scientific and Technical Information of China (English)

    徐群; 张建业; 王勤友; 张世训; 司桂玲

    2003-01-01

    Objective To evaluate the status of eight RHD specific exons in 131 Han Chinese blood donors who were classified as RhD-negative by serological methods and explore the genomic structure of RHD gene among the Han Chinese. The Rh blood group system has the highest prevalence of polymorphisms among human blood group systems and is clinically significant in transfusion medicine. The Rh antigens are expressed on polypeptides encoded by two highly homologous genes, RHD and RHCE. Recent molecular studies have shown that the RhD-negative trait could be generated by multiple genetic mechanisms and is ethnic group-dependent.Methods The polymerase chain reaction using-sequence specific primers (PCR-SSP) was used to amplify exons 2, 3, 4, 5, 6, 7, 9 and 10 of RHD gene and exons 1, 2 and 5 of RHCE gene, as well as intron 4 in each of them.Results The 131 cases of RhD-negative phenotypes consisted of 60 ccee, 58 Ccee, 5 ccEe, 5 CcEe and 3 CCee. Among them, 83 with the Rh ccee or ccEe phenotypes (63.4%) lacked the eight RHD exons indicated above, while 26 cases with the Rh Ccee, CCee, CcEe phenotypes (19.9%) had all the RHD exons examined. Twenty-two individuals with the Ccee, CCee, CcEe phenotypes (16.8%) carried at least one RHD exon. The phenotypes of the RhD negative individuals carrying the RHD gene were Rh CC or Cc, but not cc. Conclusions Three classes of RhD-negative polymorphisms among a population of Han Chinese were observed. Antigen association analysis suggested the existence of a novel class of RhD-negative associated haplotype in Han Chinese. This haplotype consisted of a normal RHCE allele and a nonfunctional RHD gene. It may be beneficial to redefine the RhD-negative blood group among Chinese populations upon clarification of the mechanisms of RHD gene expression and RhD antigen immunization.

  14. Occurrence of ABO And RhD Incompatibility with Rh Negative Mothers.

    Science.gov (United States)

    Izetbegovic, Sebija

    2013-12-01

    Hemolytic disease of the newborn was first described in the medical literature 1609, when it was diagnosed in one French housewife. In 1932 Diamond and colleagues described the mutual relationship of fetal hydrops, jaundice, anemia and erythoblastosis, which was later called fetal erytroblastosis. Hemolytic disease of the newborn (HDN) in the strict sense is considered disease whose basis is accelerated immune destruction of fetal/child erythrocytes that are bound to IgG antibodies of maternal origin. These antibodies are directed against antigens of father's origin, which are present in the fetal/children's erythrocytes and that the mother's immune system recognizes them as foreign antigens. The goal is that in the period from January 1(st) 2011 to October 23(st) 2013 determine the frequency of ABO and Rh D incompatibilities in our sample of pregnant women/mothers, and to underscore the importance of regular check of ABO Rh D negative pregnant women and application specific Rh D protection. In the General Hospital "Prim. Dr. Abdulah Nakas" in Sarajevo by retrospective study are followed several relevant variables. Immune alloantibodies were detected in vivo by indirect Coombs test (ICT) with serum mother and O test erythrocytes, by direct Coombs test (DCT) with erythrocytes of a newborn. The total number of births ABO Rh D negative was 596 (14%) and ABO Rh D positive mothers 4261 (86%). Of the total number of Rh D negative mothers there was A Rh D: negative mothers 42%; O Rh D negative 33%; B Rh D: negative 17% and AB Rh D: negative 8%. Most of immune antibodies appear in mothers with O Rh D: negative blood type. The emergence of immune antibodies in the Rh D negative mothers was 1%, the appearance of ABO incompatibilities amounted to 2.3% of our sample. IN ORDER TO REDUCE THE OCCURRENCE OF ALLOIMMUNIZATION OF THE MOTHER TO ERYTHROCYTE ANTIGENS OF THE NEWBORN THAT CAN LEAD TO MAJOR COMPLICATIONS IN SUBSEQUENT PREGNANCIES OF RH D: negative mothers and HDN constant

  15. Photoemission study of the ferromagnetic Kondo system CeRh3B2

    Science.gov (United States)

    Fujimori, A.; Takahashi, T.; Okabe, A.; Kasaya, M.; Kasuya, T.

    1990-04-01

    We have studied the electronic structure of CeRh3B2, which has an anomalously high ferromagnetic ordering temperature, by photoemission and Auger-electron spectroscopy. The Ce 4f occupancy nf~=0.85 evaluated from the Ce 3d core-level photoemission spectrum indicates a moderately strong valence fluctuation in the Kondo regime. Rh d-derived valence-band photoemission spectra are found to be in good agreement with the results of band-structure calculations when a strong energy dependence of the hole lifetime is taken into account. This observation and the deviation of the Rh M4,5VV Auger spectrum from the self-convolution of the Rh d partial density of states provide evidence for electron correlation within the Rh d band of order of U=1-2 eV. We discuss a possible effect of the latter electron correlation on the ferromagnetic instability of this compound.

  16. Ghrelin action on GnRH neurons and pituitary gonadotropes might be mediated by GnIH-GPR147 system.

    Science.gov (United States)

    Celik, Onder; Celik, Nilufer; Aydin, Suleyman; Aygun, Banu Kumbak; Haberal, Esra Tustas; Kuloglu, Tuncay; Ulas, Mustafa; Aktun, Lebriz Hale; Acet, Mustafa; Celik, Sudenaz

    2016-02-01

    Acylated ghrelin (AG) effect on GnRH secretion is mediated, at least in part, by GH secreta-gogue receptor (GHS-R) which is present in the GnRH neurons. As the acylation is mandatory for binding to GHS-R, unacylated isoform of ghrelin (UAG) action on gonadotropin secretion is likely to be mediated by other receptors or mediators that have not been identified yet. UAG, therefore, may act partially via a GHS-R-independent mechanism and inhibitory impact of UAG on GnRH neurons may be executed via modulation of other neuronal networks. Ghrelin and gonadotropin inhibitory hormone (GnIH), two agonistic peptides, have been known as important regulators of reproductive events. Potential impact of ghrelin on the activity of GnIH neurons is not exactly known. Both GnIH and ghrelin are potent stimulators of food intake and inhibitors of gonadotropin release. By binding G-protein coupled GnIH receptor (GnIH-R), GPR147, which is located in the human gonadotropes and GnRh neurons, GnIH exerts an inhibitory effect on both GnRH neurons and the gonadotropes. The GnIH-GPR147 system receives information regarding the status of energy reservoir of body from circulating peptides and then transfers them to the kisspeptin-GnIH-GnRH network. Due to wide distribution of this network in brain GnIH neurons may project on ghrelin neurons in the arcuate nucleus and contribute to the regulation of UAG's central effects or vice versa. Together, the unidentified ghrelin receptor in the hypothalamus and hypophysis may be GnIH-R. Therefore, it is reasonable that ghrelin may act on both hypothalamus and hypophysis via GnIH-GPR147 system to block gonadotropin synthesis and secretion.

  17. The MNS glycophorin variant GP.Mur affects differential erythroid expression of Rh/RhAG transcripts.

    Science.gov (United States)

    Hsu, K; Kuo, M-S; Yao, C-C; Cheng, H-C; Lin, H-J; Chan, Y-S; Lin, M

    2017-08-24

    The band 3 macrocomplex (also known as the ankyrin-associated complex) on the red cell membrane comprises two interacting subcomplexes: a band 3/glycophorin A subcomplex, and a Rh/RhAG subcomplex. Glycophorin B (GPB) is a component of the Rh/RhAG subcomplex that is also structurally associated with glycophorin A (GPA). Expression of glycophorin B-A-B hybrid GP.Mur enhances band 3 expression and is associated with lower levels of Rh-associated glycoprotein (RhAG) and Rh polypeptides. The goal of this study was to determine whether GP.Mur influenced erythroid Rh/RhAG expression at the transcript level. GP.Mur was serologically determined in healthy participants from Taitung County, Taiwan. RNA was extracted from the reticulocyte-enriched fraction of peripheral blood, followed by reverse transcription and quantitative PCR for RhAG, RhD and RhCcEe. Quantification by real-time PCR revealed significantly fewer RhAG and RhCcEe transcripts in the reticulocytes from subjects with homozygous GYP*Mur. Independent from GYP.Mur, both RhAG and RhD transcript levels were threefold or higher than that of RhCcEe. Also, in GYP.Mur and the control samples alike, direct quantitative associations were observed between the transcript levels of RhAG and RhD, but not between that of RhAG and RhCcEe. Erythroid RhD and RhCcEe were differentially expressed at the transcript levels, which could be related to their different degrees of interaction or sensitivity to RhAG. Further, the reduction or absence of glycophorin B in GYP.Mur erythroid cells affected transcript expressions of RhAG and RhCcEe. Thus, GPB and GP.Mur differentially influenced Rh/RhAG expressions prior to protein translation. © 2017 International Society of Blood Transfusion.

  18. The Rh allele frequencies in Gaza city in Palestine

    Directory of Open Access Journals (Sweden)

    Skaik Younis

    2011-01-01

    Full Text Available Background: The Rh blood group system is the second most clinically significant blood group system. It includes 49 antigens, but only five (D, C, E, c and e are the most routinely identified due to their unique relation to hemolytic disease of the newborn (HDN and transfusion reactions. Frequency of the Rh alleles showed variation, with regard to race and ethnic. Objectives: The purpose of the study was to document the Rh alleles′ frequencies amongst males (M and females (F in Gaza city in Palestine. Materials and Methods: Two hundred and thirty-two blood samples (110 M and 122 F were tested against monoclonal IgM anti-C,anti-c, anti-E, anti-e and a blend of monoclonal/polyclonal IgM/IgG anti-D. The expected Rh phenotypes were calculated using gene counting method. Results: The most frequent Rh antigen in the total sample was e, while the least frequent was E.The order of the combined Rh allele frequencies in both M and F was CDe > cDe > cde > CdE > cDE > Cde > CDE. A significant difference was reported between M and F regarding the phenotypic frequencies (P < 0.05. However, no significance (P > 0.05 was reported with reference to the observed and expected Rh phenotypic frequencies in either M or F students. Conclusion: It was concluded that the Rh antigens, alleles and phenotypes in Gaza city have unique frequencies, which may be of importance to the Blood Transfusion Center in Gaza city and anthropology.

  19. White blood cell counting system

    Science.gov (United States)

    1972-01-01

    The design, fabrication, and tests of a prototype white blood cell counting system for use in the Skylab IMSS are presented. The counting system consists of a sample collection subsystem, sample dilution and fluid containment subsystem, and a cell counter. Preliminary test results show the sample collection and the dilution subsystems are functional and fulfill design goals. Results for the fluid containment subsystem show the handling bags cause counting errors due to: (1) adsorption of cells to the walls of the container, and (2) inadequate cleaning of the plastic bag material before fabrication. It was recommended that another bag material be selected.

  20. The H blood group system.

    Science.gov (United States)

    Scharberg, Erwin A; Olsen, Coral; Bugert, Peter

    2016-09-01

    The H blood group system, ISBT symbol H (018), consists of a single antigen (H) defined by a terminal fucose residue found on red blood cells and in secretions formed by the action of α-1,2-fucosyltransferases 1 (α2FucT1) and 2 (α2FucT2), respectively. Mutant alleles of the corresponding FUT1 and FUT2 genes result in either a H– phenotype (Bombay phenotype, Oh) or a weak H phenotype (para-Bombay, H+w). In addition, the FUT2 gene is the molecular basis of the secretor (Se) status, and homozygosity or compound heterozygosity for null alleles is associated with the nonsecretor (se) status. H– individuals have natural anti-H (mostly IgM), which can cause severe hemolytic transfusion reactions with intravascular hemolysis.

  1. Cage-forming compounds in the Ba-Rh-Ge system: from thermoelectrics to superconductivity.

    Science.gov (United States)

    Falmbigl, M; Kneidinger, F; Chen, M; Grytsiv, A; Michor, H; Royanian, E; Bauer, E; Effenberger, H; Podloucky, R; Rogl, P

    2013-01-18

    Phase relations and solidification behavior in the Ge-rich part of the phase diagram have been determined in two isothermal sections at 700 and 750 °C and in a liquidus projection. A reaction scheme has been derived in the form of a Schulz-Scheil diagram. Phase equilibria are characterized by three ternary compounds: τ(1)-BaRhGe(3) (BaNiSn(3)-type) and two novel phases, τ(2)-Ba(3)Rh(4)Ge(16) and τ(3)-Ba(5)Rh(15)Ge(36-x), both forming in peritectic reactions. The crystal structures of τ(2) and τ(3) have been elucidated from single-crystal X-ray intensity data and were found to crystallize in unique structure types: Ba(3)Rh(4)Ge(16) is tetragonal (I4/mmm, a = 0.65643(2) nm, c = 2.20367(8) nm, and R(F) = 0.0273), whereas atoms in Ba(5)Rh(15)Ge(36-x) (x = 0.25) arrange in a large orthorhombic unit cell (Fddd, a = 0.84570(2) nm, b = 1.4725(2) nm, c = 6.644(3) nm, and R(F) = 0.034). The body-centered-cubic superstructure of binary Ba(8)Ge(43)□(3) was observed to extend at 800 °C to Ba(8)Rh(0.6)Ge(43)□(2.4), while the clathrate type I phase, κ(I)-Ba(8)Rh(x)Ge(46-x-y)□(y), reveals a maximum solubility of x = 1.2 Rh atoms in the structure at a vacancy level of y = 2.0. The cubic lattice parameter increases with increasing Rh content. Clathrate I decomposes eutectoidally at 740 °C: κ(I) ⇔ (Ge) + κ(IX) + τ(2). A very small solubility range is observed at 750 °C for the clathrate IX, κ(IX)-Ba(6)Rh(x)Ge(25-x) (x ∼ 0.16). Density functional theory calculations have been performed to derive the enthalpies of formation and densities of states for various compositions Ba(8)Rh(x)Ge(46-x) (x = 0-6). The physical properties have been investigated for the phases κ(I), τ(1), τ(2), and τ(3), documenting a change from thermoelectric (κ(I)) to superconducting behavior (τ(2)). The electrical resistivity of κ(I)-Ba(8)Rh(1.2)Ge(42.8)□(2.0) increases almost linearly with the temperature from room temperature to 730 K, and the Seebeck coefficient is negative

  2. The Indian blood group system.

    Science.gov (United States)

    Xu, Q

    2011-01-01

    The Indian blood group system (ISBT: IN/023) consists of two antithetical antigens: In(a) (IN1), which is present in approximately 10 percent of some Arab populations and in 3 percent of Bombay Indians, and its allelic antigen In(b) (IN2), an antigen of high incidence in all populations. In 2007, two new high-incidence antigens were identified as belonging to the IN blood group system, namely IN3 (INFI) and IN4 (INJA). The antigens in this system are located on CD44, a single-pass membrane glycoprotein that is encoded by the CD44 gene on chromosome 11 at position p13. The biologic function of CD44 is as a leukocyte homing receptor and cellular adhesion molecule. The In(a) and In(b) polymorphism represents a 252G>C substitution of CD44, encoding R46P, and lack of IN3 and IN4 results from homozygosity for mutations encoding H85Q and T163R in the CD44 gene. The high-frequency antigen AnWj (901009) has not been assigned to the Indian system, but either is located on an isoform of CD44 or is closely associated with it.

  3. Clinical Significance of Screening of Rh-negative Blood Group and Detection of Irregular Antibody%Rh阴性血型筛查及不规则抗体检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    李岚; 伍昌林; 党鑫堂; 董洪强; 朱奕

    2011-01-01

    目的 通过Rh阴性血型筛查及不规则抗体的检测,对抗体阳性的Rh阴性患者选择相合的血液输注,并分析其临床意义.方法 采用微柱凝集法对363例RhD阴性输血患者进行抗体筛查及Rh血型鉴定,选择Rh因子相合的血液输注,对有抗-c和抗-e抗体Rh阴性患者在血源紧缺的情况下,选择O型ccdEE和CCdee表型红细胞输注,观察临床输血效果.结果 在363例RhD阴性患者中,不规则抗体阳性21例,其中抗-D抗体5例,抗-E抗体8例,抗-c抗体3例,抗-c、E抗体2例,抗-C抗体2例,抗-e抗体1例,对存在不规则抗体的患者选择Rh因子相合的血液输注,临床效果良好.结论 根据抗体筛查及Rh血型鉴定结果,选择Ph因子相合的血液输注,可避免不规则抗体的产生,提高临床输血的有效性,减少输血不良反应的发生.%Objective To choose the matching blood to Rh-negative patients who were positive for irregular antibody by the screening of Rh-negative blood group and the detection of irregular antibody, and analyze its clinical significance.Methods A total of 363 RhD-negative patients were subjected to antibody screening and Rh blood grouping by micro-column agglutination method,based on which the Rh factor-matched blood was selected for transfusion.For lack of matched blood source, the RhD-negative patients with anti-c and anti-e antibodies were transfused with erythrocytes group O of ccdEE and CCdee phenotypes.Results Of the 363 RhD-negative patients, 21 were positive for irregular antibody, including 5 for anti-D, 8 for anti-E, 3 for anti-c, 2 for anti-c and E,2 for anti-C and 1 for anti-e.The patients with irregular antibodies were transfused with selected Rh factor-matched blood, and sarisfactory clinical efficacy was observed.Conclusion By transfusion with Rh factor-matched blood selected based on antibody screening and Rh blood grouping, the production of irregular antibodies was avoided, the efficacy of clinical blood transfusion was

  4. Investigation of Yangzhou Region Blood Donors' ABO and Rh Blood Types Distribution and Correlation%扬州地区献血者ABO及Rh血型人群分布及其相关性调查

    Institute of Scientific and Technical Information of China (English)

    焦玉东; 范恩勇

    2012-01-01

    Objective To Explore Yangzhou blood donors' ABO and Rh blood groups distribution and correlation.Methods From January 2010 to June 2011,63925 samples collected by Yangzhou blood bank from unpaid blood donors on streets,were enrolled in this study.Positive and negative stereotypes of ABO blood specimens,identification and genotyping of Rh blood group were tested by U-shaped micro-plate methods.Rh negative confirmatory tests were recognized by anti-globulin method using three kinds IgG anti-D reagents from different manufacturers.Samples gene frequencies,haplotype frequencies and expected values were calculated.The phenotypic observed and expected value were compared by the X2 test,to test the Hardy-Weinberg goodness of fit.Results The gene frequencies of ABO blood group were r>p>q,phenotype distribution characteristics were O>A>B>AB; Rh phenotypes population distribution were CCDee>CcDEe>CcDee>ccDEE>ccDEe>CCDEe>ccDee,ccdee>Ccdee>CCdee>ccdEe>CcdEe>ccdEE,haplotype frequencies were CDe>cDe>cDE>CDE,cde>Cde>cdE>CdE,and gene frequencies characteristics were D>d,C>c,e>E.The difference between the expected and observed values of the ABO blood group and Rh blood group were no statistical significance (P>0.05).Hardy-Weinberg,the goodness of fit test results were good.Conclusions The distributions of ABO and Rh blood typing population in this region were in line with the Hardy-Weinberg population-based genetic equilibrium in population genetics.Through the survey of the ABO and Rh blood group distributions in Yangzhou region,we could accurately grasp the law of Yangzhou region blood donors' blood types distribution,which were contribute for unpaid blood donors recruitment,reasonable blood collection plan development,blood reserves and rare blood types establishment library.%目的 探讨扬州地区献血者ABO,Rh血型人群分布状况,并对其相关性进行调查研究.方法 选择2010年1月至2011年6月本市中心

  5. [Synthesis of GnRH analogs and their application in targeted gene delivery systems].

    Science.gov (United States)

    Iablokova, T V; Chelushkin, P S; Dorosh, M Iu; Efremov, A M; Orlov, S V; Burov, S V

    2012-01-01

    A set of GnRH analogues containing nuclear localization signal (NLS) of SV-40 virus large T-antigen have been synthesized using solid phase peptide synthesis and chemical ligation technique. Selective chemical ligation was achieved as a result of hydrazone formation in the course of interaction between NLS hydrazide and GnRH analog modified by pyruvic acid. The efficiency of synthesized peptide carriers was demonstrated in experiments with human cancer cells transfected by reporter luciferase and beta-galactosidase genes or suicide HSV-1 thymidine kinase gene. It was shown that selectivity of action on cancer cells can be achieved as a result of peptide/DNA complex penetration through the cell membrane by GnRH receptor-mediated endocytosis pathway.

  6. Rh(O)D immune globulin products for prevention of alloimmunization during pregnancy.

    Science.gov (United States)

    Aitken, Samuel L; Tichy, Eric M

    2015-02-15

    The pharmacologic properties of Rhesus (Rh) immune globulin (RhIG) and clinical data on its effectiveness in preventing Rh-antigen alloimmunization in pregnant women are reviewed. RhIG is a human plasma derivative that targets red blood cells (RBCs) positive for Rh(O) antigen (also called D antigen). In the United States and other countries, the widespread use of RhIG has markedly reduced the occurrence of hemolytic disease of the fetus and newborn (HDFN), a devastating condition caused by D-antigen sensitization of a pregnant woman via exposure to fetal RBCs (usually during detachment of the placenta in labor) that results in a maternal immune response leading to severe hemolysis in the fetus. Routine administration of RhIG at 26-30 weeks' gestation and again within 72 hours of delivery has been shown to be highly effective in preventing maternal Rh alloimmunization, with very low rates of D-antigen sensitization (in the range of 0-2.2%) reported in multiple studies of at-risk women. The four RhIG products currently available in the United States have common clinical indications but differ in certain attributes. Pharmacists can play an important role in guiding other clinicians on the rationale for the use of RhIG, important differences between products, and appropriate timing of RhIG therapy. Routine administration of RhIG to women at risk for Rh alloimmunization is clinically effective and has made HDFN a rare clinical event. The available RhIG products are not the same and should be carefully reviewed to ensure that they are administered safely. Copyright © 2015 by the American Society of Health-System Pharmacists, Inc. All rights reserved.

  7. PERCENTAGE OF SANGUIN GROUP (ABO, RH

    Directory of Open Access Journals (Sweden)

    M.Mirdamadi

    1978-09-01

    Full Text Available The ABO and Rh Blood groups of 9753 pregnant women were determined in the Amin Maternity Hospital, Isfahan, Iran, from March 23, 1976 to July 11, 1977. Their ABO blood group frequencies were calculated as follows: 0=38.20%, A=30.67%, B=23.84% and AB=7.26%. Also, 90.02% of the women were Rh positive and the rest, namely, 9.98% were Rh negative.

  8. Magnetism and superconductivity in the system Ce1-xLaxRh3B2

    Science.gov (United States)

    Malik, S. K.; Umarji, A. M.; Shenoy, G. K.; Aldred, A. T.; Niarchos, D. G.

    1985-10-01

    The compound CeRh3B2 is magnetically ordered with a low saturation moment of ~0.4 μB per formula unit and an unusually large Curie temperature of 115 K while isostructural LaRh3B2 is superconducting with a transition temperature of ~2.3 K. Magnetic and superconducting studies on the series of compounds Ce1-xLaxRh3B2 reveal that the magnetic state persists for 0<=x<=0.8. The magnetic moment per Ce ion goes through a maximum and the Curie temperature decreases as La is substituted for Ce. On the La-rich side, superconductivity is destroyed even with 1% replacement of La by Ce, indicating a strong coupling of Ce 4f moments with conduction-electron spins. The temperature dependence of the upper critical field of LaRh3B2 has been measured and is discussed in terms of Werthammer-Helfand-Hohenberg theory.

  9. Anomalous electrical resistivity of the Kondo system Ce(Rh1-xCox)3B2

    Science.gov (United States)

    Ku, H. C.; Yu, H.

    1986-08-01

    Electrical resistivity measurements have been carried out on the high-Curie-temperature ferromagnetic compound CeRh3B2 (TC=110-115 K). The temperature dependence of the electrical resistivity ρ(T) during the initial cooldown above TC gives the first solid indication of the Kondo-like behavior in this system. However, the resisitivity is irreversible above TC when warming up from low temperature and ρ(T) approaches the previous reported form. This irreversibility is closely related to microscopic cracks created by the strong internal magnetic field of the ferromagnetic state and was not observed in the nonmagnetic compound CeCo3B2 with the same hexagonal structure. Further proof of this Kondo state can be obtained in the study of the pseudoternary system Ce(Rh1-xCox)3B2 where the resistivity increases with decreasing temperature during the initial cooldown and a local minimum Kondo anomaly was observed. The magnetic state is rapidly broken up with the replacement of Rh by Co and the resistivity anomaly disappears after the disappearance of ferromagnetic order.

  10. Direct evidence of a nonorthogonal magnetization configuration in single crystalline Fe(1-x)Co(x)/Rh/Fe/Rh(001) System.

    Science.gov (United States)

    Yildiz, F; Przybylski, M; Kirschner, J

    2009-10-02

    Tetragonal distortion in Fe1-xCox alloy films grown epitaxially on Rh(001) substrates results in a strong perpendicular magnetic anisotropy. Since the perpendicular magnetic anisotropy varies with the Fe1-xCox film composition, one can grow multilayer structures with ferromagnetic films sequentially showing either an in-plane (e.g., Fe) or out-of-plane (e.g., Fe0.5Co0.5) easy-magnetization axis. The Rh spacers mediate an interlayer coupling which couples the magnetizations either ferromagnetically or antiferromagnetically, depending on the spacer thickness. When the anisotropy energy is compatible to the coupling, it produces nonorthogonal magnetization configurations which vary under a small change of the external magnetic field.

  11. Determination of the equilibrium miscibility gap in the Pd-Rh alloy system using metal nanopowders obtained by decomposition of coordination compounds

    Energy Technology Data Exchange (ETDEWEB)

    Shubin, Yu.V., E-mail: shubin@niic.nsc.ru; Plyusnin, P.E.; Korenev, S.V.

    2015-02-15

    Highlights: • The Pd-Rh phase diagram has been experimentally reinvestigated. • The true equilibrium was achieved with the two-way approach. • The critical point of the miscibility gap lie at 58 at.% Rh and 820 °C. - Abstract: The Pd-Rh phase diagram has been reinvestigated in the subsolidus region using X-ray diffraction, scanning and transmission electron microscopy. The true equilibrium at the miscibility boundary was achieved with the two-way approach. Nanosized powders of metastable solid solutions and two-phase palladium-rhodium mixtures were used to shorten the time required to equilibrate the system. The initial samples were prepared by decomposition of coordination compounds [Pd(NH{sub 3}){sub 2}Cl{sub 2}], [Rh(NH{sub 3}){sub 5}Cl]Cl{sub 2}, [Pd(NH{sub 3}){sub 4}]{sub 3}[Rh(NO{sub 2}){sub 6}]{sub 2} and [Pd(NH{sub 3}){sub 4}][Rh(NH{sub 3})(NO{sub 2}){sub 5}]. The obtained phase diagram exhibits miscibility gap wider than generally accepted with the critical point of solubility at 58 at.% Rh and 820 °C.

  12. Poor performance of BACTEC NR 730 blood culture system in early detection of Neisseria meningitidis.

    OpenAIRE

    1989-01-01

    During an 8-month period at Children's Hospital, Oakland, Calif., a 9% rate for positive blood culture for children with Neisseria meningitidis meningitis was identified. The blood culture system used in each case was the BACTEC NR 730. This rate seemed significantly lower than previous rates (33 to 55%) (P.R. Dodge and M.N. Swartz, N. Engl. J. Med. 272:1003-1010, 1965; A.L. Hoyne and R.H. Brown, Ann. Intern. Med. 28:248-259, 1948; S. Levin and M.B. Painter, Ann. Intern. Med. 64:1049-1057, 19...

  13. A novel phosphate ligand used for the Rh-catalyzed hydroformylation of cyclohexene in a thermoregulated PEG biphase system

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A novel phosphate ligand, tri-(methoxyl polyethylene glycol)-phosphate (TMPGPA), has been synthesized and used in the Rhcatalyzed hydroformylation of cyclohexene in a thermoregulated PEG biphase system. Under the optimized conditions,pressure = 5 Mpa (H2:CO = 1:1), P/Rh = 10 (molar ratio), reaction time = 4 h and temperature = 120 ℃, the conversion of cyclohexene and the yield of aldehyde are 99%. The catalyst retained in PEG phase can be easily separated from the organic phase containing product by simple phase separation and reused ten times without obvious loss in activity.

  14. Anisotropic B-T Phase Diagram of Non-Kramers System PrRh2Zn20

    Science.gov (United States)

    Yoshida, Taichi; Machida, Yo; Izawa, Koichi; Shimada, Yuki; Nagasawa, Naohiro; Onimaru, Takahiro; Takabatake, Toshiro; Gourgout, Adrien; Pourret, Alexandre; Knebel, Georg; Brison, Jean-Pascal

    2017-04-01

    We have investigated the low temperature quadrupolar phenomena of the non-Kramers system PrRh2Zn20 under magnetic fields in the [100] and [110] directions. Our experiments reveal the B-T phase diagram of PrRh2Zn20 involving four electronic states regardless of the field direction, namely, a non-Fermi liquid (NFL) state, an antiferro-quadrupolar (AFQ) ordered state, a novel heavy-fermion (HF) state, and a field-induced singlet (FIS) state. In the wide range of the NFL state, the resistivity can be well scaled by a characteristic temperature, suggesting the realization of the quadrupole Kondo effect. The HF state exhibits a Fermi liquid behavior with a large A coefficient of the T2 term in the resistivity, suggesting the formation of nontrivial heavy quasi-particles. The FIS state results from the considerable splitting of a non-Kramers doublet by a magnetic field. The phase diagram shows a large anisotropy with respect to the field direction. It is found that the anisotropy of the phase diagram can be explained in terms of that of the energy splitting of the non-Kramers doublet by a magnetic field. This indicates that the low temperature properties of PrRh2Zn20 are governed by the non-Kramers doublet, namely, quadrupole degrees of freedom. Since a similar phase diagram has been obtained for the related compound PrIr2Zn20, it is expected that the B-T phase diagram constructed in this work is universal throughout non-Kramers systems.

  15. Lactobacillus rhamnosus accelerates zebrafish backbone calcification and gonadal differentiation through effects on the GnRH and IGF systems.

    Directory of Open Access Journals (Sweden)

    Matteo A Avella

    Full Text Available Endogenous microbiota play essential roles in the host's immune system, physiology, reproduction and nutrient metabolism. We hypothesized that a continuous administration of an exogenous probiotic might also influence the host's development. Thus, we treated zebrafish from birth to sexual maturation (2-months treatment with Lactobacillus rhamnosus, a probiotic species intended for human use. We monitored for the presence of L. rhamnosus during the entire treatment. Zebrafish at 6 days post fertilization (dpf exhibited elevated gene expression levels for Insulin-like growth factors -I and -II, Peroxisome proliferator activated receptors -α and -β, VDR-α and RAR-γ when compared to untreated-10 days old zebrafish. Using a gonadotropin-releasing hormone 3 GFP transgenic zebrafish (GnRH3-GFP, higher GnRH3 expression was found at 6, 8 and 10 dpf upon L. rhamnosus treatment. The same larvae exhibited earlier backbone calcification and gonad maturation. Noteworthy in the gonad development was the presence of first testes differentiation at 3 weeks post fertilization in the treated zebrafish population -which normally occurs at 8 weeks- and a dramatic sex ratio modulation (93% females, 7% males in control vs. 55% females, 45% males in the treated group. We infer that administration of L. rhamnosus stimulated the IGF system, leading to a faster backbone calcification. Moreover we hypothesize a role for administration of L. rhamnosus on GnRH3 modulation during early larval development, which in turn affects gonadal development and sex differentiation. These findings suggest a significant role of the microbiota composition on the host organism development profile and open new perspectives in the study of probiotics usage and application.

  16. Genotyping study of RHD variants among RhD-negative blood donors, Panyu district%番禺地区RHD变异体基因分型研究

    Institute of Scientific and Technical Information of China (English)

    谢敬文; 邓诗桢; 严康峰; 蓝文莉; 莫锦政; 马伟文

    2013-01-01

    目的 研究分析番禺地区RHD变异体基因分型特征.方法 采用微量板法对2010年11月~2011年8月到本站参加献血的26 172名次献血者进行RhD阴性筛查;采用抗球蛋白法对初筛RhD阴性的标本进行确认,采用吸收放散试验对经抗球蛋白法确认为RhD阴性的标本进行Del表型筛选;采用RH基因变异体基因分型检测试剂盒(PCR-SSP法)对献血者模板DNA进行扩增,对扩增产物进行电泳分析,根据电泳图谱判断RHD基因分型.对血清学与基因分型结果不符的标本进行测序分析.结果 初筛检出60名RhD阴性,经抗球蛋白法确认59例为RhD阴性,阴性频率为0.23%;吸收放散试验检出10例Del表型.60例初筛阴性的基因分型检测发现40例RHDEXONl~ 10全缺失基因型,发生频率约为67.8%;10例RHD 1227A DEL基因型,发生频率约为16.9%;7例部分D,发生频率约为11.9%;1例RHD弱D15基因型,发生频率约为1.2%;2例RHD基因分型阳性需作进一步碱基测序分析.结论 本地区血清学RhD阴性献血者中,存在RHD 1227A DEL、RHD-CE(2-9)-D,RHD-CE(5)-D、RHD-CE(6-9)-D和RHD弱D15等基因型,血清学结合基因分型能更准确鉴定RhD血型.%Objective To study and analyze genotyping characteristics of RHD gene variants among Panyu RhD-negative blood donors. Methods Use micro-plate method to screen RhD-negative samples among 26 172 blood donors from November 2010 to August 2011 in Panyu Blood Centre. Using anti-globulin method to identify RhD-negative screening samples , using adsorption and elution test to screen De phenotype among the above RhD-negative-identified samples, using RH gene variants genotyping assay kit (PCR-SSP) to amplify template DNA of blood donors and the amplified products were analyzed by electrophoresis. And RHD genotyping was determined according to the polyacrylamide gel electrophoresis. The samples that the serological and genotyping results were not matching were sequenced. Results 60 Rh

  17. Use of a pneumatic tube system for delivery of blood bank products and specimens.

    Science.gov (United States)

    Tanley, P C; Wallas, C H; Abram, M C; Richardson, L D

    1987-01-01

    This study evaluated the effect of pneumatic tube transport on blood bank specimens and products. No important differences were found between aliquots transported in the tube system and those stored in the laboratory as controls. ABO, Rh, antibody detection or identification, direct antiglobulin testing, and elution were studied. Further, no differences in plasma hemoglobin and potassium concentration were found between units of whole blood and packed cells handled in either manner. Platelet counts in platelet concentrates were not decreased and coagulation factor levels in units of fresh-frozen plasma and cryoprecipitate did not decrease after pneumatic transport. The system tested is currently providing expeditious transport of specimens and blood between blood banks and patient care areas.

  18. Blood as integral system of an organism

    Directory of Open Access Journals (Sweden)

    Майя Розметовна Верголяс

    2016-02-01

    Full Text Available Relevance of use of hematological blood parameters for monitoring as markers of various physiological and pathological processes is substantiated. It is shown that the blood is an important system of the body, has all the reactive characteristics of tissues, its sensitivity to pathological stimuli is very high. The reaction of the organism to the irritation of toxic or infectious nature manifests itself in the change of quantitative composition of peripheral blood cells

  19. Embedded programmable blood pressure monitoring system

    Science.gov (United States)

    Hasan, Md. Mahmud-Ul; Islam, Md. Kafiul; Shawon, Mehedi Azad; Nowrin, Tasnuva Faruk

    2010-02-01

    A more efficient newer algorithm of detecting systolic and diastolic pressure of human body along with a complete package of an effective user-friendly embedded programmable blood pressure monitoring system has been proposed in this paper to reduce the overall workload of medical personals as well as to monitor patient's condition more conveniently and accurately. Available devices for measuring blood pressure have some problems and limitations in case of both analog and digital devices. The sphygmomanometer, being analog device, is still being used widely because of its reliability and accuracy over digital ones. But it requires a skilled person to measure the blood pressure and obviously not being automated as well as time consuming. Our proposed system being a microcontroller based embedded system has the advantages of the available digital blood pressure machines along with a much improved form and has higher accuracy at the same time. This system can also be interfaced with computer through serial port/USB to publish the measured blood pressure data on the LAN or internet. The device can be programmed to determine the patient's blood pressure after each certain interval of time in a graphical form. To sense the pressure of human body, a pressure to voltage transducer is used along with a cuff in our system. During the blood pressure measurement cycle, the output voltage of the transducer is taken by the built-in ADC of microcontroller after an amplifier stage. The recorded data are then processed and analyzed using the effective software routine to determine the blood pressure of the person under test. Our proposed system is thus expected to certainly enhance the existing blood pressure monitoring system by providing accuracy, time efficiency, user-friendliness and at last but not the least the 'better way of monitoring patient's blood pressure under critical care' all together at the same time.

  20. Catalytic hydrodechlorination of trichloroethylene in a novel NaOH/2-propanol/methanol/water system on ceria-supported Pd and Rh catalysts.

    Science.gov (United States)

    Cobo, Martha; Becerra, Jorge; Castelblanco, Miguel; Cifuentes, Bernay; Conesa, Juan A

    2015-08-01

    The catalytic hydrodechlorination (HDC) of high concentrations of trichloroethylene (TCE) (4.9 mol%, 11.6 vol%) was studied over 1%Pd, 1%Rh and 0.5%Pd-0.5%Rh catalysts supported on CeO2 under conditions of room temperature and pressure. For this, a one-phase system of NaOH/2-propanol/methanol/water was designed with molar percentages of 13.2/17.5/36.9/27.6, respectively. In this system, the alcohols delivered the hydrogen required for the reaction through in-situ dehydrogenation reactions. PdRh/CeO2 was the most active catalyst for the degradation of TCE among the evaluated materials, degrading 85% of the trichloroethylene, with alcohol dehydrogenation rates of 89% for 2-propanol and 83% for methanol after 1 h of reaction. Fresh and used catalysts were characterized by Transmission Electron Microscopy (TEM), X-ray Photoelectron Spectroscopy (XPS), and Thermogravimetric analysis (TGA). These results showed important differences of the active phase in each catalyst sample. Rh/CeO2 had particle sizes smaller than 1 nm and the active metal was partially oxidized (Rh(0)/Rh(+δ) ratio of 0.43). This configuration showed to be suitable for alcohols dehydrogenation. On the contrary, Pd/CeO2 showed a Pd completed oxidized and with a mean particle size of 1.7 nm, which seemed to be unfavorable for both, alcohols dehydrogenation and TCE HDC. On PdRh/CeO2, active metals presented a mean particle size of 2.7 nm and more reduced metallic species, with ratios of Rh(0)/Rh(+δ) = 0.67 and Pd(0)/Pd(+δ) = 0.28, which showed to be suitable features for the TCE HDC. On the other hand, TGA results suggested some deposition of NaCl residues over the catalyst surfaces. Thus, the new reaction system using PdRh/CeO2 allowed for the degradation of high concentrations of the chlorinated compound by using in situ hydrogen liquid donors in a reaction at room temperature and pressure.

  1. Blood monitoring systems and methods thereof

    Science.gov (United States)

    Mir, Jose (Inventor); Zander, Dennis (Inventor)

    2012-01-01

    A blood monitoring system is capable of monitoring the blood of a subject in vivo. The blood monitoring system comprises: 1) an array of movable microneedle micromachined within associated wells; 2) array of motion actuators able to move each needle in and out of their associated wells; 3) array of microvalves associated with each microneedle able to control the flow of air around the microneedle; 4) an array of chemical sensors inserted into patient by movable microneedles; 5) an array of inductors able to measure chemical concentration in the vicinity of inserted chemical sensors; 6) conducting vias that provide timed actuating signal signals from a control system to each motion actuator; 7) conducting vias that transmit signal produced by array of chemical sensors to the control system for processing, although the blood monitoring system can comprise other numbers and types of elements in other configurations.

  2. Particle Swarm Optimization Algorithm for Optimizing Assignment of Blood in Blood Banking System

    OpenAIRE

    Olusanya, Micheal O.; Arasomwan, Martins A.; Aderemi O. Adewumi

    2015-01-01

    This paper reports the performance of particle swarm optimization (PSO) for the assignment of blood to meet patients’ blood transfusion requests for blood transfusion. While the drive for blood donation lingers, there is need for effective and efficient management of available blood in blood banking systems. Moreover, inherent danger of transfusing wrong blood types to patients, unnecessary importation of blood units from external sources, and wastage of blood products due to nonusage necessi...

  3. 21 CFR 864.9145 - Processing system for frozen blood.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Processing system for frozen blood. 864.9145... Blood and Blood Products § 864.9145 Processing system for frozen blood. (a) Identification. A processing system for frozen blood is a device used to glycerolize red blood cells prior to freezing to...

  4. High pressure studies on the ferromagnetic dense Kondo systems CeRh3B2 and UCu2Ge2

    Science.gov (United States)

    Cornelius, A. L.; Schilling, J. S.; Endstra, T.; Mydosh, J. A.

    1994-07-01

    The dependence of the Curie temperature of the anomalous ferromagnets UCu2Ge2 and CeRh3B2 on hydrostatic pressure to 11 GPa is determined using a diamond-anvil cell loaded with dense helium as pressure medium. A sensitive primary/secondary coil system allows the detection of the ferromagnetic transition in the ac susceptibility for tiny samples with less than 1 μ mass. The Curie temperatures of the above two compounds, Tc≊110 K and 118 K, both increase initially under pressure but pass through maxima at 8 GPa and 2 GPa, respectively, before falling rapidly at higher pressures. We take this as evidence that both compounds behave as dense Kondo system, where Tc depends on the exchange coupling J according to a magnetic phase diagram originally proposed by Doniach.

  5. Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: is mammalian GnRH being expressed?

    Science.gov (United States)

    Montaner, A D; Gonzalez, O; Paz, D A; Affanni, J M; Somoza, G M

    2000-08-01

    In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no crossreactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera.

  6. Die Steuerung der Aktivität des Response Regulators CiaR des Zwei-Komponenten Systems CiaRH in Streptococcus pneumoniae

    OpenAIRE

    Marx, Patrick

    2013-01-01

    Das Zwei-Komponenten System CiaRH beeinflusst mit der β-Lactamresistenz, Kompetenz, Autolyse, Bakteriocinproduktion und Virulenz eine Vielzahl an Phänotypen in Streptococcus pneumoniae und ist daher von großer physiologischer Bedeutung. Es setzt sich aus der membrangebundenen Sensorkinase CiaH und dem cytoplasmatisch lokalisierten Response Regulator CiaR zusammen. Das CiaRH System ist unter sehr vielen Wachstumsbedingungen aktiv. CiaH ist allerdings für die Aktivierung von CiaR unter bestimmt...

  7. A Guide to Terminology for Rh Immunoprophylaxis.

    Science.gov (United States)

    Sandler, S Gerald; Queenan, John T

    2017-09-01

    Rh immunoprophylaxis for Rh-negative women requires an understanding of terminology used for Rh blood typing laboratory reports. The pathophysiology of Rh hemolytic disease of the fetus and newborn was elucidated by studies in rhesus monkeys. Subsequent studies revealed that the human blood group antigen responsible for Rh hemolytic disease of the newborn (D antigen) is related to, but different from, the rhesus monkey antigen. Weak expression of the D antigen on red cells, originally termed D, is currently reported by laboratories as a "serologic weak D phenotype," which can be further defined by RHD genotyping to be either a weak D type or a partial D phenotype. Weak D types 1, 2, or 3 are molecularly defined RHD weak D types, which have an adequate number of intact D antigens to be managed safely as Rh-positive. Partial D phenotypes result from mutations causing loss of one or more D epitopes. Most persons with a partial D phenotype have sufficient D antigen to type as Rh-positive. Some women with a partial D phenotype are detected as serologic weak D phenotypes by routine Rh typing. Whether they type as Rh-positive or serologic weak D phenotype, after being exposed to Rh-positive red cells by transfusion or pregnancy, women with partial D phenotype can form anti-D antibodies and, if they do, are at risk for hemolytic disease of the fetus and newborn. A pregnant woman with a laboratory report of a serologic weak D phenotype should be further tested for her RHD genotype to resolve whether her case should be managed as Rh-positive or Rh-negative. For more than five decades, the practice of Rh immunoprophylaxis has remained unchanged in terms of the dose of Rh immune globulin and timing of injections. In contrast, advances in the science of Rh blood typing have resulted in a continuously evolving terminology, obliging obstetricians to update their vocabulary to guide their practice. The following review and glossary provide guidance for current Rh terminology and

  8. Rh Incompatibility

    Science.gov (United States)

    ... attack the baby's red blood cells. This can lead to hemolytic anemia (HEE-moh-lit-ick uh-NEE-me-uh) ... baby won't get enough oxygen. This can lead to serious problems. Severe hemolytic anemia may even be fatal to the child. Outlook ...

  9. Rh Immunoprophylaxis for Women With a Serologic Weak D Phenotype.

    Science.gov (United States)

    Virk, Mrigender; Sandler, S Gerald

    2015-01-01

    It is standard practice for pregnant RhD-negative women who have not already formed anti-D to receive antepartum Rh immunoprophylaxis and, if they deliver an RhD-positive neonate, to receive postpartum Rh immunoprophylaxis. An estimated 0.6% to 1.0% of white women have red blood cells that express a serologic weak D phenotype. Of these women, approximately 80% will have a weak D type 1, 2, or 3 that could be managed safely as RhD-positive. Surveys of laboratory practice reveal a lack of standards for interpreting the RhD type for women with a serologic weak D and for determining their need for Rh immunoprophylaxis. RhD genotyping is recommended to determine the molecular basis of serologic weak D phenotypes in pregnant women as a basis for determining their candidacy for Rh immunoprophylaxis.

  10. Variant RH alleles and Rh immunisation in patients with sickle cell disease

    Science.gov (United States)

    Sippert, Emilia; Fujita, Claudia R.; Machado, Debora; Guelsin, Glaucia; Gaspardi, Ane C.; Pellegrino, Jordão; Gilli, Simone; Saad, Sara S.T.O.; Castilho, Lilian

    2015-01-01

    Background Alloimmunisation is a major complication in patients with sickle cell disease (SCD) receiving red blood cell (RBC) transfusions and despite provision of Rh phenotyped RBC units, Rh antibodies still occur. These antibodies in patients positive for the corresponding Rh antigen are considered autoantibodies in many cases but variant RH alleles found in SCD patients can also contribute to Rh alloimmunisation. In this study, we characterised variant RH alleles in 31 SCD patients who made antibodies to Rh antigens despite antigen-positive status and evaluated the clinical significance of the antibodies produced. Materials and methods RHD and RHCE BeadChip™ from BioArray Solutions and/or amplification and sequencing of exons were used to identify the RH variants. The serological features of all Rh antibodies in antigen-positive patients were analysed and the clinical significance of the antibodies was evaluated by retrospective analysis of the haemoglobin (Hb) levels before and after transfusion; the change from baseline pre-transfusion Hb and the percentage of HbS were also determined. Results We identified variant RH alleles in 31/48 (65%) of SCD patients with Rh antibodies. Molecular analyses revealed the presence of partial RHD alleles and variant RHCE alleles associated with altered C and e antigens. Five patients were compound heterozygotes for RHD and RHCE variants. Retrospective analysis showed that 42% of antibodies produced by the patients with RH variants were involved in delayed haemolytic transfusion reactions or decreased survival of transfused RBC. Discussion In this study, we found that Rh antibodies in SCD patients with RH variants can be clinically significant and, therefore, matching patients based on RH variants should be considered. PMID:24960646

  11. Variant RH alleles and Rh immunisation in patients with sickle cell disease.

    Science.gov (United States)

    Sippert, Emilia; Fujita, Claudia R; Machado, Debora; Guelsin, Glaucia; Gaspardi, Ane C; Pellegrino, Jordão; Gilli, Simone; Saad, Sara S T O; Castilho, Lilian

    2015-01-01

    Alloimmunisation is a major complication in patients with sickle cell disease (SCD) receiving red blood cell (RBC) transfusions and despite provision of Rh phenotyped RBC units, Rh antibodies still occur. These antibodies in patients positive for the corresponding Rh antigen are considered autoantibodies in many cases but variant RH alleles found in SCD patients can also contribute to Rh alloimmunisation. In this study, we characterised variant RH alleles in 31 SCD patients who made antibodies to Rh antigens despite antigen-positive status and evaluated the clinical significance of the antibodies produced. RHD and RHCE BeadChip™ from BioArray Solutions and/or amplification and sequencing of exons were used to identify the RH variants. The serological features of all Rh antibodies in antigen-positive patients were analysed and the clinical significance of the antibodies was evaluated by retrospective analysis of the haemoglobin (Hb) levels before and after transfusion; the change from baseline pre-transfusion Hb and the percentage of HbS were also determined. We identified variant RH alleles in 31/48 (65%) of SCD patients with Rh antibodies. Molecular analyses revealed the presence of partial RHD alleles and variant RHCE alleles associated with altered C and e antigens. Five patients were compound heterozygotes for RHD and RHCE variants. Retrospective analysis showed that 42% of antibodies produced by the patients with RH variants were involved in delayed haemolytic transfusion reactions or decreased survival of transfused RBC. In this study, we found that Rh antibodies in SCD patients with RH variants can be clinically significant and, therefore, matching patients based on RH variants should be considered.

  12. One System for Blood Program Information Management

    OpenAIRE

    Gero, Michael G.; Klickstein, Judith S.; Hurst, Timm M.

    1980-01-01

    A system which integrates the diverse functions of a Blood Program within one structure is being assembled at the American National Red Cross Blood Services, Northeast Region. When finished, it will provide technical support for collection scheduling, donor recruitment, recordkeeping, laboratory processing, inventory management, HLA typing and matching, distribution, and administration within the Program. By linking these applications, a reporting structure useful to top management will be pr...

  13. 本院ABO及RhD血型需求调查

    Institute of Scientific and Technical Information of China (English)

    王波; 陈玉瑙

    2015-01-01

    Objective To investigate the need of ABO blood group and RhD blood transfusions in patients with potentially Nanyang region, and be able to effectively guide the blood supply to the body to science collecting blood, making the blood to meet the needs of clinical applications. Methods Microlab Star 8CH sampling system, to our hospital inpatients were analyzed 83,958 cases and RhD blood group ABO blood group. Results In 83,958 cases of patients, including patients with type O blood accounted for 42.43%, B-type blood of patients was 27.02%, A-type blood of patients was 24.63%, AB blood type of patients was 5.92%, RhD-negative patients was 0.31% type .Conclusion Knowing the the requirement of ABO and RhD blood group in Nanyang region, on the use of blood to save lives with a positive meaning.%目的:了解南阳地区输血患者ABO血型和RhD血型的需求,有效指导供血机构来科学采集血液,使得血液能够满足临床应用的需要。方法采用Microlab Star 8CH加样系统,对医院83958例住院患者进行ABO血型和RhD血型的分析。结果在这83958例患者中,其中O型血患者为42.43%,B型血患者为27.02%,A型血患者为24.63%,AB型血患者为5.92%,RhD型阴性患者为0.31%。结论了解南阳地区ABO及RhD血型需求情况,促进合理组织血液,对利用血液来拯救生命具有积极的意义。

  14. IBCIS:Intelligent blood cell identification system

    Institute of Scientific and Technical Information of China (English)

    Adnan Khashman

    2008-01-01

    The analysis of blood cells in microscope images can provide useful information concerning the health of patients.There are three major blood cell types,namely,erythrocytes (red),leukocytes (white),and platelets.Manual classification is time consuming and susceptible to error due to the different morphological features of the cells.This paper presents an intelligent system that simulates a human visual inspection and classification of the three blood cell types.The proposed system comprises two phases:The image preprocessing phase where blood cell features are extracted via global pattern averaging,and the neural network arbitration phase where training is the first and then classification is carried out.Experimental results suggest that the proposed method performs well in identifying blood cell types regardless of their irregular shapes,sizes and orientation,thus providing a fast,simple and efficient rotational and scale invariant blood cell identification system which can be used in automating laboratory reporting.

  15. Automated microscopy system for peripheral blood cells

    Science.gov (United States)

    Boev, Sergei F.; Sazonov, Vladimir V.; Kozinets, Gennady I.; Pogorelov, Valery M.; Gusev, Alexander A.; Korobova, Farida V.; Vinogradov, Alexander G.; Verdenskaya, Natalya V.; Ivanova, Irina A.

    2000-11-01

    The report describes the instrument ASPBS (Automated Screening of Peripheral Blood Cells) designed for an automated analysis of dry blood smears. The instrument is based on computer microscopy and uses dry blood smears prepared according to the standard Romanovskii-Giemza procedure. In comparison with the well-known flow cytometry systems, our instrument provides more detailed information and offers an opporunity of visualizing final results. The basic performances of the instrument are given. Software of this instrument is based on digital image processing and image recognition procedures. It is pointed out that the instrument can be used as a fairly universal tool in scientific research, public demonstrations, in medical treatment, and in medical education. The principle used as the basis of the instrument appeared adequate for creating an instrument version serviceable even during space flights where standard manual procedures and flow cytometry systems fail. The benefit of the use of the instrument in clinical laboratories is described.

  16. Feedback actions of estradiol on GnRH secretion during the follicular phase of the estrous cycle.

    Science.gov (United States)

    Karsch, F J; Evans, N P

    1996-01-01

    The pattern of GnRH secretion during the follicular phase of the estrous cycle of sheep is characterized by an initial marked change in episodic secretion (increased frequency and decreased amplitude) followed by a massive and sustained discharge-the preovulatory GnRH surge. Studies employing a physiological model for the follicular phase have revealed that estradiol has profound and complex feedback effects on GnRH release during the preovulatory period. These include both quantitative effects on pulses (stimulation of frequency, inhibition of amplitude) and qualitative effects (altering pulse shape, stimulating interpulse secretion), in addition to inducing a preovulatory GnRH surge. In stimulating the surge, estradiol causes a highly characteristic change in the minute-to-minute pattern of GnRH in hypophyseal portal blood. Initially, a strictly episodic pattern gives way to one in which GnRH is consistently elevated between pulses. Then, following enhancement of both pulsatile and interpulse components, GnRh becomes extremely high and variable for the majority of the surge. From this point, a regular and well organized pulse pattern is not apparent. The characteristic time course of GnRH at surge onset provides insight into possible mechanistic changes in the GnRH neurosecretory system. Such changes include quantitative and qualitative alterations in the pulse generating mechanism, recruitment of a surge specific population of GnRH neurones, morphologic alterations in GnRH neurones and neighboring cells, and changes in efficiency or route of delivery of GnRH from its site of release to the portal vasculature. These possibilities, while untested and speculative, provide a conceptual framework for future research.

  17. Semaphorin Signaling in the Development and Function of the Gonadotropin Hormone-Releasing Hormone (GnRH System

    Directory of Open Access Journals (Sweden)

    Andrea eMessina

    2013-09-01

    Full Text Available The semaphorin proteins are among the best-studied families of guidance cues, contributing to morphogenesis and homeostasis in a wide range of tissue types. The major semaphorin receptors are plexins and neuropilins, however other receptors and co-receptors are capable to mediate signaling by semaphorins. These guidance proteins were originally identified as growth cone collapsing factors or as inhibitory signals, crucial for nervous system development. Since those seminal discoveries, the list of functions of semaphorins has rapidly grown. Over the past few years, a growing body of data indicates that semaphorins are involved in the regulation of the immune and vascular systems, in tumor growth/cancer cell metastasis and in neural circuit formation. Recently there has been increasing emphasis on research to determine the potential influence of semaphorins on the development and homeostasis of hormone systems and how circulating reproductive hormones regulate their expression and functions. Here, we focus on the emerging role of semaphorins in the development, differentiation and plasticity of unique neurons that secrete gonadotropin-releasing hormone (GnRH, which are essential for the acquisition and maintenance of reproductive competence in all vertebrates. Genetic evidence is also provided showing that insufficient semaphorin signaling contributes to some forms of reproductive disorders in humans, characterized by the reduction or failure of sexual competence.Finally, we will review some studies with the goal of highlighting how the expression of semaphorins and their receptors might be regulated by gonadal hormones in physiological and pathological conditions.

  18. Gliotransmission by Prostaglandin E2: a prerequisite for GnRH neuronal function?

    Directory of Open Access Journals (Sweden)

    Jerome eClasadonte

    2011-12-01

    Full Text Available Over the past four decades it has become clear that prostaglandin E2 (PGE2, a phospholipid-derived signaling molecule, plays a fundamental role in modulating the GnRH neuroendocrine system and in shaping the hypothalamus. In this review, after a brief historical overview, we highlight studies revealing that PGE2 released by glial cells such as astrocytes and tanycytes is intimately involved in the active control of GnRH neuronal activity and neurosecretion. Recent evidence suggests that hypothalamic astrocytes surrounding GnRH neuronal cell bodies may respond to neuronal activity with an activation of the erbB receptor tyrosine-kinase signaling, triggering the release of PGE2 as a chemical transmitter from the glia themselves, and, in turn, leading to the feedback regulation of GnRH neuronal activity. At the GnRH neurohemal junction, in the median eminence of the hypothalamus, PGE2 is released by tanycytes in response to cell-cell signaling initiated by glial cells and vascular endothelial cells. Upon its release, PGE2 causes the retraction of the tanycyte end feet enwrapping the GnRH nerve terminals, enabling them to approach the adjacent pericapillary space and thus likely facilitating neurohormone diffusion from these nerve terminals into the pituitary portal blood. In view of these new insights, we suggest that synaptically-associated astrocytes and perijunctional tanycytes are integral modulatory elements of GnRH neuronal function at the cell soma/dendrite and nerve terminal levels, respectively.

  19. RH精炼过程计算机控制系统的发展%Development of computer control system for RH vacuum refining process

    Institute of Scientific and Technical Information of China (English)

    张春霞; 刘浏; 刘昆华; 刘良田; 黄波

    2000-01-01

    随着RH真空精炼设备和工艺技术的不断进步,RH真空精炼设备已在众多炉外精炼中起到不可替代的重要作用,适用于大量生产优质超低碳钢。实践证明,在RH处理过程中,采用计算机自动控制系统,对稳定提高钢质量,实现高效、低耗生产具有重要意义。对此,结合国内外RH真空精炼过程计算机自动控制系统的发展,对RH过程计算机控制系统的构成、特点和控制模型的功能等进行了综合评述。通过开发武钢1#RH-KTB精炼过程控制模型的实践,提出对我国发展RH过程计算机控制的几点建议。%RH vacuum degassing technology is applied worldwide due to its continuous improvement in equipment and process technique. The practices prove that it is very necessary for improving the quality of liquid steel, increasing yield and reducing energy consumption to adopt computer control system in RH vacuum refining process. Combining the development of computer automatic control system for RH refining process home and abroad, the constitution, features and functions of control model are reviewed and some suggestions for developing the computer control technology of RH process are put forward.

  20. The Diego blood group system: a review.

    Science.gov (United States)

    Figueroa, Dolores

    2013-01-01

    The Diego blood group system (DI) currently encompasses 22 antigens. Three of the antigens are of high prevalence and the other 19 are of low prevalence. The antigens of the Diego blood group system are carried on the erythroid band 3 protein anion exchanger 1 (AE1), the product of a single gene, SLC4A1 (solute carrier family 4, anion exchanger, member 1). AE1 is a member of a family of three anion exchangers or transporters expressed in a variety of tissues. This protein is involved in carbon dioxide transport from tissues to lungs. It is also found in the kidney,where it is involved in acid secretion. Antibodies to Diego system antigens with the exception of anti-Dia, -Dib, -Wra, -ELO and-DISK do not seem to be of clinical significance for transfusion or of importance in hemolytic disease of the fetus and newborn.

  1. Copper-based alloys, crystallographic and crystallochemical parameters of alloys in binary systems Cu-Me (Me=Co, Rh, Ir, Cu, Ag, Au, Ni, Pd, Pt)

    Energy Technology Data Exchange (ETDEWEB)

    Porobova, Svetlana, E-mail: porobova.sveta@yandex.ru; Loskutov, Oleg, E-mail: lom58@mail.ru [Tomsk State University of Architecture and Building, Russia, Tomsk, 2 Solyanaya sq, Tomsk, 634003 (Russian Federation); Markova, Tat’jana, E-mail: patriot-rf@mail.ru [Siberian State Industrial University. 42 Kirov St., Novokuznetsk, 654007 (Russian Federation); Klopotov, Vladimir, E-mail: vdklopotov@mail.ru [Research Tomsk Polytechnic University, 30 Lenin Ave., Tomsk, 634050 (Russian Federation); Klopotov, Anatoliy, E-mail: klopotovaa@tsuab.ru [Tomsk State University of Architecture and Building, Russia, Tomsk, 2 Solyanaya sq, Tomsk, 634003 (Russian Federation); National Research Tomsk State University, 36, Lenin Ave., Tomsk, 634050 (Russian Federation); Vlasov, Viktor, E-mail: vik@tsuab.ru [Tomsk State University of Architecture and Building, Russia, Tomsk, 2 Solyanaya sq, Tomsk, 634003 (Russian Federation); Research Tomsk Polytechnic University, 30 Lenin Ave., Tomsk, 634050 (Russian Federation)

    2016-01-15

    The article presents the results of the analysis of phase equilibrium of ordered phases in binary systems based on copper Cu- Me (where Me - Co, Rh, Ir, Ag, Au, Ni, Pd, Pt) to find correlations of crystallochemical and crystallographic factors. It is established that the packing index in disordered solid solutions in binary systems based on copper is close to the value of 0.74 against the background of an insignificant deviation of atomic volumes from the Zen’s law.

  2. Reduction of magnetic damping and isotropic coercivity and increase of saturation magnetization in Rh-incorporated CoIr system

    Science.gov (United States)

    Wong, H. S.; He, S. K.; Chung, H. J.; Zhang, M. S.; Cher, Kelvin; Low, Melvin; Zhou, T. J.; Yang, Y.; Wong, S. K.

    2016-11-01

    Replacing Ir with Rh in a CoIr system possessing negative uniaxial magnetocrystalline anisotropy (K u ) substantially reduces its magnetic damping and coercivity by more than half while retaining its high negative K u . Moreover, a higher saturation magnetization (M s ) and more isotropic coercivity are achieved. Such material development makes it particularly suitable for use as the soft underlayer (SUL) of magnetic recording media for reducing noise, and as the oscillation layer of a spin-torque oscillator (STO) for achieving higher oscillation frequency, larger AC magnetic field and lower driving current, which can be readily integrated with the current recording head for microwave-assisted magnetic recording. Finally, we recommend a composite free layer by coupling CoIr with a spin polarizer (Co or Co/Cu/Co) for the enhancement of the spin-polarization rate and, therefore, the improvement of STO efficiency. These could pave the way for CoIr-based materials to be implemented in devices requiring a negative Ku with low damping and high ‘softness’, such as oscillators.

  3. Hydrogen Adsorption on Pt, Rh and Pt-Rh Electrodes

    Institute of Scientific and Technical Information of China (English)

    贾梦秋; A.M.Meretskyi

    2005-01-01

    The hydrogen adsorption on Pt-Rh alloys in sulfuric acid aqueous solutions was studied by the method of cathode pulses. Hydrogen adsorption on the electrode with all ratio of alloy components (ωRh = 0-100%) is well described by the Temkin logarithmic isotherm. The surface coverage by adsorbed hydrogen at the same potential is decreased with increasing content of rhodium in the system. A linear dependence of adsorption peak potential on the alloy compositions in the case of weakly bonded adsorbed hydrogen is established. Hydrogen adsorption heat as a function of surface coverage for Pt-Rh-electrodes was obtained. The shape of the current-potential curve and position of the weakly bonded hydrogen adsorption on the potential scale are all related to alloy compositions, thus can serve as the basis for the determination surface composition of allovs.

  4. Association of ABO and Rh blood groups to HBV, HCV infections among blood donors in a blood bank of tertiary care teaching hospital in Southern India: A retrospective study

    Directory of Open Access Journals (Sweden)

    Sreedhar Babu KV

    2015-07-01

    Conclusion: In this study conducted to determine the predominant blood group antigen and its association with HBV and HCV seroreactivity, there was no association between blood group antigens with these infections. [Int J Res Med Sci 2015; 3(7.000: 1672-1676

  5. 安钢RH精炼工艺中的质谱炉气分析系统%Mass spectrometric furnace gas analysis system in Angang RH refining process

    Institute of Scientific and Technical Information of China (English)

    毛尽华; 郝贵奇; 胡少成; 郭永谦; 王超刚

    2012-01-01

    对于高品质钢的冶炼,RH真空循环脱气工艺功能强大,技术效益显著,目前在国内的应用越来越普遍.采用质谱炉气分析系统在线分析RH工艺过程的气体成分,结合流量、温度测控技术,可以实现对RH工艺的过程优化和终点控制.本文介绍了安钢第二炼轧厂RH工艺中采用的质谱炉气分析系统的组成、安装布局、分析性能和现场的工作模式等,并采用吹氧脱碳处理工艺、不吹氧脱碳处理工艺和脱气处理工艺对炉气分析系统进行测试.结果表明:该系统能够实时、连续和准确地反映处理过程中各成分随时间的变化,60米采样距离时系统总滞后时间小于40s,炉气成分变化过程同处理工艺完全吻合.可预见采用炉气分析技术,能为RH精炼工艺的终点控制及工艺的实时调整提供判据,从而缩短RH冶炼时间,提升冶炼品质.%For the smelting of high-quality steel, the RH vacuum circulation degassing process has been widely used in China due to its powerful processing function and significant benefits. The gas composition in RH process can be on-line analyzed with mass spectrometric gas analysis system. In combination with gas flow and temperature measurement and control technology, the process optimization and end-point control of RH process can be realized. In this paper, the structure, installation layout, analysis performance and work mode in the field of mass spectrometric gas analysis system used in RH process of No. 2 steel-making and rolling plant of Anyang Iron and Steel Group was introduced. Three RH processes, including oxygen blowing decarburization treatment, decarburization treatment and degassing treatment, have been used to test the performance of the analyzing system. The results showed that, this system could monitor the changes of each component in the exhaust gas of RH real-time, continuously and accurately. The total latency of the system was less than 40s for the sampling

  6. Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    José Antonio Muñoz-Cueto

    2013-04-01

    Full Text Available Several evidences supported the existence of melatonin effects on reproductive system in fish. In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 µg/g body mass in male specimens. The brain mRNA transcript levels of the three GnRH forms and the five GnRH receptors present in this species were determined by real time quantitative PCR. Our findings revealed day–night variations in the brain expression of GnRH-1, GnRH-3 and several GnRH receptors (dlGnRHR-II-1c, -2a, which exhibited higher transcript levels at mid-light compared to mid-dark phase of the photocycle. Moreover, an inhibitory effect of melatonin on the nocturnal expression of GnRH-1, GnRH-3, and GnRH receptors subtypes 1c, 2a and 2b was also demonstrated. Interestingly, the inhibitory effect of melatonin affected the expression of hypophysiotrophic GnRH forms and GnRH receptors that exhibit day–night fluctuations, suggesting that exogenous melatonin reinforce physiological mechanisms already established. These interactions between melatoninergic and GnRH systems could be mediating photoperiod effects on reproductive and other rhythmic physiological events in the European sea bass.

  7. Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax

    Science.gov (United States)

    Servili, Arianna; Herrera-Pérez, Patricia; del Carmen Rendón, María; Muñoz-Cueto, José Antonio

    2013-01-01

    Several evidences supported the existence of melatonin effects on reproductive system in fish. In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 μg/g body mass) in male specimens. The brain mRNA transcript levels of the three GnRH forms and the five GnRH receptors present in this species were determined by real time quantitative PCR. Our findings revealed day–night variations in the brain expression of GnRH-1, GnRH-3 and several GnRH receptors (dlGnRHR-II-1c, -2a), which exhibited higher transcript levels at mid-light compared to mid-dark phase of the photocycle. Moreover, an inhibitory effect of melatonin on the nocturnal expression of GnRH-1, GnRH-3, and GnRH receptors subtypes 1c, 2a and 2b was also demonstrated. Interestingly, the inhibitory effect of melatonin affected the expression of hypophysiotrophic GnRH forms and GnRH receptors that exhibit day–night fluctuations, suggesting that exogenous melatonin reinforce physiological mechanisms already established. These interactions between melatoninergic and GnRH systems could be mediating photoperiod effects on reproductive and other rhythmic physiological events in the European sea bass. PMID:23567273

  8. Quantum Criticality and Lifshitz Transition in the Ising System CeRu2Si2: Comparison with YbRh2Si2

    Science.gov (United States)

    Pourret, Alexandre; Aoki, Dai; Boukahil, Mounir; Brison, Jean-Pascal; Knafo, William; Knebel, Georg; Raymond, Stephane; Taupin, Mathieu; Ōnuki, Yoshichika; Flouquet, Jacques

    2014-06-01

    New thermoelectric power (TEP) measurements on prototype heavy-fermion compounds close to magnetic quantum criticality are presented. The highly sensitive technique of TEP is an unique tool to reveal Fermi surface instabilities, referred here as Lifshitz transitions. The first focus is on the Ising CeRu2Si2 series. Doping CeRu2Si2 with Rh produces a decoupling between the first order metamagnetic transition and the pseudo-metamagnetism observed in the pure compound. Comparison is made with the case of YbRh2Si2 which is often considered as the archetype of local quantum criticality by contrast to CeRu2Si2, taken as an example of spin-density wave criticality. Up to now for ferromagnetic materials showing ferromagnetic wings, no simple case appears where the Fermi surface is preserved between the ferromagnetic and paramagnetic phases. An open issue is the consequence of Lifshitz transitions on superconductivity in these multiband systems.

  9. RH Mixing observable?

    CERN Document Server

    Achiman, Yoav

    1999-01-01

    Asymmetric mass matrices can induce large RH mixings. Those are non -measurable in the SM but are there and play an important role in its extensions. The RH rotations are in particular relevant for the proton decay, neutrino properties and baryon asymmetry. E.g. large RH mixings lead to kaon dominated proton decay even without SUSY and could be the reason for a large neutrino mixing. By studying those phenomena one can learn about the RH rotation matrices and this can reduce considerably the arbitrariness in the present fermionic mass study.

  10. Antioxidant status, immune system, blood metabolites and carcass ...

    African Journals Online (AJOL)

    Antioxidant status, immune system, blood metabolites and carcass characteristic of broiler ... African Journal of Biotechnology ... rhizome powder (TP) on performance, blood metabolite, immune system, antioxidant status, and relative weight of ...

  11. [An integrated system of blood pressure measurement with bluetooth communication].

    Science.gov (United States)

    Wang, Wei; Wang, Jing; Sun, Hongyang; Xu, Zuyang; Chai, Xinyu

    2012-07-01

    The development of the integrated blood pressure system with bluetooth communication function is introduced. Experimental results show that the system can complete blood pressure measurement and data transmission wireless effectively, which can be used in m-Health in future.

  12. Efficient Nd Promoted Rh Catalysts for Vapor Phase Methanol Carbonylation

    Institute of Scientific and Technical Information of China (English)

    Shu Feng ZHANG; Qing Li QIAN; Ping Lai PAN; Yi CHEN; Guo Qing YUAN

    2005-01-01

    A Nd promoted-Rh catalysts supported on polymer-derived carbon beads for vapor-phase methanol carbonylation was developed. Rh-Nd bimetallic catalysts obviously have higher activity than that of supported Rh catalyst under similar reaction condition. The difference between the activity of above two catalyst systems is clearly caused by the intrinsic properties generated by the introduction of Nd.

  13. Compound heterozygosity of two novel RHAG alleles leads to a considerable disruption of the Rh complex.

    Science.gov (United States)

    Polin, Helene; Pelc-Klopotowska, Monika; Danzer, Martin; Suessner, Susanne; Gabriel, Christian; Wilflingseder, Julia; Żmudzin, Alicja; Orzińska, Agnieszka; Guz, Katarzyna; Michalewska, Bogumila; Brojer, Ewa

    2016-04-01

    The Rhesus (Rh) complex consists of a core comprising the Rh proteins (RhD/RhCE) and the Rh-associated glycoprotein (RhAG) with accessory chains (GPB, LW, CD47). Molecular defects of the RHAG gene may cause a regulator Rhnull phenotype without Rh antigen expression or a Rhmod phenotype with decreased Rh antigen expression. Blood samples of a donor with strongly diminished Rh antigens and five family members were analyzed by serological phenotyping, flow cytometry, molecular testing, and gene expression analysis of Rh complex candidate genes. RHAG sequencing identified a missense mutation, c.241G>C (p.Gly81Arg) and a splice site mutation, c.640 + 3del14, among the cohort. Compound heterozygosity of these novel alleles identified in the propositus and two siblings gave rise to a strongly diminished expression of RhAG, Rh, and CD47 antigens on the RBC surface. The Rhmod phenotype was caused by a novel RHAG splice site mutation in association with a non-functional allele. The primary depression of RhAG is most likely due to posttranslational events that affect the interaction and processing of the RhAG glycoprotein and gave rise to a secondary depression of RhD, RhCE, and CD47, the major members of the Rh complex. © 2016 AABB.

  14. Electronic Structure of CeRh3B2: A Large f-f Direct Mixing System

    Science.gov (United States)

    Takegahara, Katsuhiko; Harima, Hisatomo; Kasuya, Tadao

    1985-12-01

    The standard self-consistent APW band calculations for LaRh3B2 and CeRh3B2, which crystallize in the hexagonal CeCo3B2 type structure, have been done using the local density approximation. Among the 4f states, the magnetic quantum number lz{=}0 state has the large mixing with the conduction bands and thus is the ground state of the crystal field. For CeRh3B2, the band calculation, in which the orbital degeneracy of 4f states is lifted by introducing the lz dependent pseudo-potential, is also done for the ferromagnetic alignment of the 4f state with lz{=}0. Results show that, due to the large (ffσ), this 4f state has strong dispersion along the c-direction creating 4f holes on the \\varGamma-M-K plane in the Brillouin zone. This 4f hole formation is the origin of the anomalous magnetic properties and various anomalous properties are explained consistently on this model.

  15. Particle swarm optimization algorithm for optimizing assignment of blood in blood banking system.

    Science.gov (United States)

    Olusanya, Micheal O; Arasomwan, Martins A; Adewumi, Aderemi O

    2015-01-01

    This paper reports the performance of particle swarm optimization (PSO) for the assignment of blood to meet patients' blood transfusion requests for blood transfusion. While the drive for blood donation lingers, there is need for effective and efficient management of available blood in blood banking systems. Moreover, inherent danger of transfusing wrong blood types to patients, unnecessary importation of blood units from external sources, and wastage of blood products due to nonusage necessitate the development of mathematical models and techniques for effective handling of blood distribution among available blood types in order to minimize wastages and importation from external sources. This gives rise to the blood assignment problem (BAP) introduced recently in literature. We propose a queue and multiple knapsack models with PSO-based solution to address this challenge. Simulation is based on sets of randomly generated data that mimic real-world population distribution of blood types. Results obtained show the efficiency of the proposed algorithm for BAP with no blood units wasted and very low importation, where necessary, from outside the blood bank. The result therefore can serve as a benchmark and basis for decision support tools for real-life deployment.

  16. Particle Swarm Optimization Algorithm for Optimizing Assignment of Blood in Blood Banking System

    Directory of Open Access Journals (Sweden)

    Micheal O. Olusanya

    2015-01-01

    Full Text Available This paper reports the performance of particle swarm optimization (PSO for the assignment of blood to meet patients’ blood transfusion requests for blood transfusion. While the drive for blood donation lingers, there is need for effective and efficient management of available blood in blood banking systems. Moreover, inherent danger of transfusing wrong blood types to patients, unnecessary importation of blood units from external sources, and wastage of blood products due to nonusage necessitate the development of mathematical models and techniques for effective handling of blood distribution among available blood types in order to minimize wastages and importation from external sources. This gives rise to the blood assignment problem (BAP introduced recently in literature. We propose a queue and multiple knapsack models with PSO-based solution to address this challenge. Simulation is based on sets of randomly generated data that mimic real-world population distribution of blood types. Results obtained show the efficiency of the proposed algorithm for BAP with no blood units wasted and very low importation, where necessary, from outside the blood bank. The result therefore can serve as a benchmark and basis for decision support tools for real-life deployment.

  17. Nocturnal variations in peripheral blood flow, systemic blood pressure, and heart rate in humans

    DEFF Research Database (Denmark)

    Sindrup, J H; Kastrup, J; Christensen, H

    1991-01-01

    was associated with a 30-40% increase in blood flow rate and a highly significant decrease in mean arterial blood pressure and heart rate (P less than 0.001 for all). Approximately 100 min after the subjects went to sleep an additional blood flow rate increment (mean 56%) and a simultaneous significant decrease......Subcutaneous adipose tissue blood flow rate, together with systemic arterial blood pressure and heart rate under ambulatory conditions, was measured in the lower legs of 15 normal human subjects for 12-20 h. The 133Xe-washout technique, portable CdTe(Cl) detectors, and a portable data storage unit...... were used for measurement of blood flow rates. An automatic portable blood pressure recorder and processor unit was used for measurement of systolic blood pressure, diastolic blood pressure, and heart rate every 15 min. The change from upright to supine position at the beginning of the night period...

  18. Ultrasound systems for blood velocity estimation

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt

    1998-01-01

    color image of velocity at up to 20 to 60 frames a second. Both measurements are performedby repeatedly pulsing in the same direction and then usethe correlation from pulse to pulse to determine the velocity.The paper gives a simple model for the interactionbetween the ultrasound and the moving blood......Medical ultrasound scanners can be used both for displayinggray-scale images of the anatomy and for visualizing theblood flow dynamically in the body.The systems can interrogate the flow at a single position in the bodyand there find the velocity distribution over time. They can also show adynamic....... The calculation of the velocity distribution is then explainedalong with the different physical effects influencing the estimation.The estimation of mean velocities using auto- andcross-correlation for color flow mapping is also described....

  19. Dynamical Systems, Cytokine Storms, and Blood Filtration

    Science.gov (United States)

    Foster, Glenn; Hubler, Alfred

    2008-03-01

    Various infections and non-infectious diseases can trigger immune cells and the proteins (cytokines) the cells use to communicate with each other to be caught in a positive feedback loop; this ``cytokine storm'' is frequently fatal. By examining the network of cytokine-immune cell interactions we will illustrate why anti-mediator drugs have been generally ineffective in stopping this feedback. A more effective approach may be to try and reduce interactions by dampening many signals at once by filtering the cytokines out of the blood directly (think dialysis). We will argue that feedback on an out of control nonlinear dynamical system is easier to understand than its normal healthy state and apply filtration to a toy model of immune response.

  20. Intelligent micro blood typing system using a fuzzy algorithm

    Science.gov (United States)

    Kang, Taeyun; Lee, Seung-Jae; Kim, Yonggoo; Lee, Gyoo-Whung; Cho, Dong-Woo

    2010-01-01

    ABO typing is the first analysis performed on blood when it is tested for transfusion purposes. The automated machines used in hospitals for this purpose are typically very large and the process is complicated. In this paper, we present a new micro blood typing system that is an improved version of our previous system (Kang et al 2004 Trans. ASME, J. Manuf. Sci. Eng. 126 766, Lee et al 2005 Sensors Mater. 17 113). This system, fabricated using microstereolithography, has a passive valve for controlling the flow of blood and antibodies. The intelligent micro blood typing system has two parts: a single-line micro blood typing device and a fuzzy expert system for grading the strength of agglutination. The passive valve in the single-line micro blood typing device makes the blood stop at the entrance of a micro mixer and lets it flow again after the blood encounters antibodies. Blood and antibodies are mixed in the micro mixer and agglutination occurs in the chamber. The fuzzy expert system then determines the degree of agglutination from images of the agglutinated blood. Blood typing experiments using this device were successful, and the fuzzy expert system produces a grading decision comparable to that produced by an expert conducting a manual analysis.

  1. Antenatal prophylaxis of Rh isoimmunization: 28-weeks'-gestation service program.

    Science.gov (United States)

    Bowman, J M; Pollock, J M

    1978-03-18

    Two (0.18%) of 1086 Rh-negative primigravidas or multigravidas treated similarly in all previous pregnancies, who were given a single injection of Rh immune globulin (300 mug) at 28 weeks' gestation and subsequently were delivered of Rh-positive babies, had demonstrable Rh isoimmunization at the time of that injection and must be considered "logistic" failures of antenatal prophylaxis. The remaining 1084 (who were treated again after delivery) had no evidence of Rh isoimmunization at delivery and none of the 512 screened at 6 months after delivery appeared to be immunized. If the 28th-week injection had not been protective, one would have expected 14 of the 1084 to have been demonstrably Rh isoimmunized and evidence of Rh isoimmunization to have persisted in 6 of the 512 observed 6 months after delivery.Six of 719 Rh-negative multigravidas who had not received Rh immune globulin after previous pregnancies or had been treated only after delivery showed evidence of Rh isoimmunization despite a single injection of Rh immune globulin at 28 weeks in a subsequent pregnancy. In three of the six the cause was most likely "sensibilization" due to previous exposure to Rh-positive blood or an untreated Rh-positive pregnancy. in 3 of the remaining 716 (0.42%) there may have been true failure of antenatal Rh prophylaxis administered at the 28th week. One would have expected this figure to be 12 of 716 if antenatal Rh prophylaxis at 28 weeks' gestation were totally unsuccessful.It is concluded that a single intramuscular injection of Rh immune globulin, 300 mug, is 88% effective in preventing Rh isoimmunization during pregnancy in Rh-negative primigravidas and in multigravidas treated antenatally in all previous pregnancies, and is 75% effective in preventing Rh isoimmunization in Rh-negative multigravidas untreated during previous pregnancies. The majority of failures are due to Rh isoimmunization during pregnancy prior to antenatal prophylaxis at 28 weeks.

  2. Inhibitive effect of cremophor RH40 or tween 80-based self-microemulsiflying drug delivery system on cytochrome P450 3A enzymes in murine hepatocytes.

    Science.gov (United States)

    Rao, Zichao; Si, Luqin; Guan, Yanbin; Pan, Hongping; Qiu, Jun; Li, Gao

    2010-10-01

    This study examined the effect of self-microemulsiflying drug delivery system (SMEDDS) containing Cremophor RH40 or Tween 80 at various dilutions on cytochrome P450 3A (CYP3A) enzymes in rat hepatocytes, with midazolam serving as a CYP3A substrate. The particle size and zeta potential of microemulsions were evaluated upon dilution with aqueous medium. In vitro release was detected by a dialysis method in reverse. The effects of SMEDDS at different dilutions and surfactants at different concentrations on the metabolism of MDZ were investigated in murine hepatocytes. The cytotoxicity of SMEDDS at different dilutions was measured by LDH release and MTT technique. The effects of SMEDDS on the CYP3A enzymes activity were determined by Western blotting. Our results showed that dilution had less effect on the particle size and zeta potential in the range from 1:25 to 1:500. The MDZ was completely released in 10 h. A significant decrease in the formation of 1'-OH-MDZ in rat hepatocytes was observed after treatment with both SMEDDS at dilutions ranging from 1:50 to 1:250 and Cremophor RH 40 or Tween 80 at concentrations ranging from 0.1% to 1% (w/v), with no cytotoxicity observed. A significant decrease in CYP3A protein expression was observed in cells by Western blotting in the presence of either Cremophor RH40 or Tween 80-based SMEDDS at the dilutions ranging from 1:50 to 1:250. This study suggested that the excipient inhibitor-based formulation is a potential protective platform for decreasing metabolism of sensitive drugs that are CYP3A substrates.

  3. Performance of the digene LQ, RH and PS HPVs genotyping systems on clinical samples and comparison with HC2 and PCR-based Linear Array

    Directory of Open Access Journals (Sweden)

    Godínez Jose M

    2011-11-01

    Full Text Available Abstract Background Certain Human Papillomaviruses (HPVs are the infectious agents involved in cervical cancer development. Detection of HPVs DNA is part of the cervical cancer screening protocols and HPVs genotyping has been proposed for its inclusion in these preventive programs. The aim of this study was to evaluate three novel genotyping tests, namely Qiagen LQ, RH and PS, in clinical samples with and without abnormalities. For this, 305 cervical samples were processed and the results of the evaluated techniques were compared with those obtained in the HPVs diagnostic process in our lab, by using HC2 and Linear Array (LA technologies. Results The concordances and kappa statistics (k for each technique compared with HC2 were 98.69% (k = 0.94 for LQ, 98.03% (k = 0.91 for RH and 91.80% (k = 0.82 for PS. There was a very good agreement in HPVs type-specific concordance for the most prevalent types HPV16 (kappa range = 0.83-0.90, HPV18 (k.r.= 0.74-0.80 and HPV45 (k.r.= 0.82-0.90. Conclusions The three tests showed an overall good concordance for HPVs detection when compared with HR-HC2 system. LQ and RH rendered lower detection rate for multiple infections than LA genotyping. However, our understanding of the clinical significance of multiple HPVs infections is still incomplete and therefore the relevance of the lower ability to detect multiple infections needs to be evaluated.

  4. Hemolysis associated with pneumatic tube system transport for blood samples.

    Science.gov (United States)

    Kara, Hasan; Bayir, Aysegul; Ak, Ahmet; Degirmenci, Selim; Akinci, Murat; Agacayak, Ahmet; Marcil, Emine; Azap, Melih

    2014-01-01

    The frequency of hemolysis of blood samples may be increased by transport in a pneumatic tube system. The purpose of this study was to evaluate the effect of pneumatic tube system transport on hemolysis of blood samples. Blood samples were transported from the emergency department to the hospital laboratory manually by hospital staff (49 patients) or with a pneumatic tube system (53 patients). The hemolysis index and serum chemistry studies were performed on the blood samples and compared between the different methods of transport. The blood samples that were transported by the pneumatic tube system had a greater frequency of hemolysis and greater mean serum potassium and median creatinine, aspartate aminotransferase, and lactate dehydrogenase levels than samples transported manually. Blood samples transported from the emergency department to the hospital laboratory by a pneumatic tube system may have a greater frequency of hemolysis than samples transported manually. This may necessitate repeat phlebotomy and cause a delay in completing the laboratory analysis.

  5. Operating theater blood transaction system. A "virtual" blood transfusion service that brings the blood bank to the operating table.

    Science.gov (United States)

    Wong, K F; Lee, A W; Hui, H L; Chang, F K; Mak, C S; Kwan, A M

    1999-10-01

    We describe an operating theater blood transaction system (OTBTS) that is a novel computer software system incorporating electronic crossmatch and the concept of a "self-service" blood banking system in the operating theater. Through this system, the surgeons and the anesthetists can issue blood units for intraoperative transfusion for the patients with a negative antibody screen without the need for a porter service or pneumatic tube system. Since implementation of the OTBTS, the time for obtaining compatible blood for intraoperative transfusion has been reduced from 20 to 30 minutes to around 1 minute. Furthermore, the crossmatch-transfusion ratio was reduced to 1.05. The 23% of patients who required extra blood units (i.e., more than originally anticipated) during surgery further benefited from the system. The blood stock reserved for patients undergoing surgery was reduced by 20%. Therefore, the OTBTS is a system that can greatly enhance the efficiency and safety of intraoperative transfusion and can also save workforce resources.

  6. What's Blood?

    Science.gov (United States)

    ... Rh" because scientists found it while studying Rhesus monkeys. If your blood is positive, you have this ... doctor. © 1995- The Nemours Foundation. All rights reserved. Images provided by The Nemours Foundation, iStock, Getty Images, ...

  7. Lack of association between Rh status, Rh immune globulin in pregnancy and autism.

    Science.gov (United States)

    Miles, Judith H; Takahashi, T Nicole

    2007-07-01

    Though causes of autism are considered largely genetic, considerable concern remains that exposure to Rh immune globulin (RhIg), which until 2001 in the United States contained the preservative thimerosal, can cause autism. To determine whether mothers of children with autism are more likely to be Rh negative (Rh(-)) or to have received RhIg preserved with thimerosal, which is 49.6% ethyl mercury, we surveyed families of children with an autism spectrum disorder (ASD) ascertained through a University-based autism clinic considered free of ascertainment biases related to type of autism or severity. Between 2004 and 2006, 305 mothers of 321 children with an ASD agreed to participate in a telephone interview. Analysis of complete records including the blood group status and RhIg exposure of 214 families showed that Rh(-) status is no higher in mothers of children with autism than in the general population, exposure to antepartum RhIg, preserved with thimerosal is no higher for children with autism and pregnancies are no more likely to be Rh incompatible. This was also true for autism subgroups defined by behavioral phenotype, gender, IQ, regressive onset, head circumference, dysmorphology, birth status, essential, or complex phenotype. These findings support the consensus that exposure to ethylmercury in thimerosal is not the cause of the increased prevalence of autism. These data are important not only for parents in this country but also for the international health community where thimerosal continues to be used to preserve multi-dose vials which in turn makes vaccines affordable.

  8. Distribution of ABO and rhesus (D blood group antigens among blood donors at a tertiary care teaching hospital blood bank in south India

    Directory of Open Access Journals (Sweden)

    Suresh B

    2015-04-01

    Full Text Available Background: The ABO and Rhesus (Rh blood group systems are important for transfusion of blood and its components, organ transplantation, genetic studies and in medico-legal issues. Despite the long list of several other blood groups discovered so far, the knowledge and distribution of ABO and Rh-D blood group are essential for effective management of blood bank inventory. Methods: We retrospectively studied the distribution of ABO and Rh blood group antigens in donors presenting to our tertiary care teaching hospital blood bank in south India during the period January 2007 to August 2014. Blood group was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results: A total of 49,110 donor samples were tested during the study period for ABO grouping and Rh-D typing. Out of these 96.9% were males. The frequency of O, B, A, AB and Bombay blood groups were 41.7%, 32.2% 20%, 6.1% and 0.03% respectively. Rh (D positive and negative blood groups were seen in 92.8% and 7.2% respectively. The allele frequencies of the I A , IB and IO alleles were 0.1398, 0.2148 and 0.6454 respectively. In case of Rh-D group, the calculated gene frequencies for ID and Id were 0.7321 and 0.2679 respectively. Conclusion: Knowledge of blood group systems as documented in the present study helps in efficient management of blood bank and transfusion services in emergencies.

  9. Processing, testing and selecting blood components.

    Science.gov (United States)

    Jones, Alister; Heyes, Jennifer

    Transfusion of blood components can be an essential and lifesaving treatment for many patients. However, components must comply with a number of national requirements to ensure they are safe and fit for use. Transfusion of incorrect blood components can lead to mortality and morbidity in patients, which is why patient testing and blood selection are important. This second article in our five-part series on blood transfusion outlines the requirements for different blood components, the importance of the ABO and RhD blood group systems and the processes that ensure the correct blood component is issued to each patient.

  10. A Calphad-type equation of state for hydrogen gas and its application to the assessment of Rh-H system

    Energy Technology Data Exchange (ETDEWEB)

    Joubert, J.-M. [Chimie Metallurgique des Terres Rares, Institut de Chimie et des Materiaux Paris-Est, CNRS-Universite de Paris 12, 2-8 rue H. Dunant, F-94320 Thiais (France)

    2010-03-15

    A new equation of state for hydrogen gas (0 {<=} P {<=} 10{sup 11} Pa, 298 {<=} T {<=} 1000 K) has been derived and is given in an analytical form. It is compatible with the Calphad approach (i.e. it is an analytical equation of the Gibbs energy as a function of pressure). This equation has been applied to the Calphad assessment of the H-Rh system, an example of system forming a metal hydride only at a very high pressure. A set of self-consistent thermodynamic parameters describing the Gibbs energy of each phase has been obtained. The scarce experimental data available for this system, particularly the high formation pressure of the hydride, have been well reproduced which otherwise would have been impossible without considering the non-ideal behaviour of the gas phase. (author)

  11. An experience of the introduction of a blood bank automation system (Ortho AutoVue Innova) in a regional acute hospital.

    Science.gov (United States)

    Cheng, Yuk Wah; Wilkinson, Jenny M

    2015-08-01

    This paper reports on an evaluation of the introduction of a blood bank automation system (Ortho AutoVue(®) Innova) in a hospital blood bank by considering the performance and workflow as compared with manual methods. The turnaround time was found to be 45% faster than the manual method. The concordance rate was found to be 100% for both ABO/Rh(D) typing and antibody screening in both of the systems and there was no significant difference in detection sensitivity for clinically significant antibodies. The Ortho AutoVue(®) Innova automated blood banking system streamlined the routine pre-transfusion testing in hospital blood bank with high throughput, equivalent sensitivity and reliability as compared with conventional manual method.

  12. Changes in the human blood coagulating system during prolonged hypokinesia

    Science.gov (United States)

    Filatova, L. M.; Anashkin, O. D.

    1978-01-01

    Changes in the coagulating system of the blood were studied in six subjects during prolonged hypokinesia. Thrombogenic properties of the blood rose in all cases on the 8th day. These changes are explained by stress reaction due to unusual conditions for a healthy person. Changes in the blood coagulating system in the group subjected to physical exercise and without it ran a practically parallel course. Apparently physical exercise is insufficient to prevent such changes that appear in the coagulating system of the blood during prolonged hypokinesia.

  13. Enthalpies of Formation of Noble Metal Binary Alloys Bearing Rh or Ir

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The modified embedded atom method proposed by authors has been applied to calculating the enthalpies of formationof random alloys and the ordered intermetallic compounds for noble metal binary systems bearing Rh or lr. The presentresults are in good agreement with those of Miedema theory, available experiments and the first-principles quantummechanics calculations. The present results indicate that Cu-Rh, Cu-lr, Ag-Rh, Ag-lr, Au-Rh, Au-lr, Pd-Rh and Pd-lrsystems are repulsive, however, Ni-Rh, Ni-lr, Pt-lr, Pt-Rh and Rh-lr systems form solid solutions and Ni-Rh, Ni-lrand Pt-Rh show ordering tendency.

  14. Eu valence and Fermi-surface development in EuX{sub 2}Si{sub 2} (X = Co, Rh, Ir) systems

    Energy Technology Data Exchange (ETDEWEB)

    Goetze, K. [Hochfeld-Magnetlabor Dresden, Helmholtz-Zentrum Dresden-Rossendorf (Germany); TU Dresden, Institut fuer Festkoerperphysik (Germany); Seiro, S.; Geibel, C.; Rosner, H.; Petzold, V. [MPI for Chemical Physics of Solids (Germany); Polyakov, A.; Wosnitza, J. [Hochfeld-Magnetlabor Dresden, Helmholtz-Zentrum Dresden-Rossendorf (Germany); Sheikin, I. [LNCMI-Grenoble (France); Suslov, A. [National High Magnetic Field Laboratory, Tallahassee (United States)

    2013-07-01

    The valence-fluctuating Eu systems EuX{sub 2}Si{sub 2}, with X being the transition metal Co, Ir, or Rh, show different types of ground states, strongly depending on X. The instability of the Eu 4f shell underlies this phenomenon and leads among other effects to different valence states ranging from Eu{sup 2+} over mixed valence and intermediate valence behavior to Eu{sup 3+}. Investigations on the structure and the magnetic behavior of EuCo{sub 2}Si{sub 2}, EuIr{sub 2}Si{sub 2}, and EuRh{sub 2}Si{sub 2} have revealed their Eu valence. Further experiments on specific heat and resistivity gave insights to magnetic ordering, electronic correlations, and possible valence fluctuations. We report about a systematic de Haas-van Alphen study on the Fermi-surface development of the EuX{sub 2}Si{sub 2} compounds in magnetic fields up to 35 T. High-quality single crystals were available for the first time. We focus on the Fermi-surface topology obtained by angle dependent measurements and discuss a comparison to band-structure calculations.

  15. Observation of large incomplete fusion in {sup 16}O + {sup 103}Rh system at {approx}3-5 MeV/nucleon

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, Unnati; Singh, Pushpendra P.; Singh, Devendra P.; Sharma, Manoj Kumar; Yadav, Abhishek [Accelerator Laboratory, Department of Physics, A.M. University, Aligarh-202002 (India); Kumar, Rakesh [NP-Group, Inter-University Accelerator Center, PO Box No. 10502, Aruna Asaf Ali Marg, New Delhi-110067 (India); Singh, B.P. [Accelerator Laboratory, Department of Physics, A.M. University, Aligarh-202002 (India)], E-mail: bpsinghamu@gmail.com; Prasad, R. [Accelerator Laboratory, Department of Physics, A.M. University, Aligarh-202002 (India)

    2008-10-01

    Incomplete fusion of {sup 16}O({approx}3-5 MeV/nucleon) with {sup 103}Rh has been investigated using measurement and analysis of excitation functions. Some pxn/{alpha}pxn-channels are found to have contribution from pre-cursor decay, which has been separated out from cumulative cross-section of evaporation residues. The xn/pxn-channels are found to be satisfactorily reproduced with the predictions of PACE4 after subtraction of pre-cursor decay contribution, in general. Sizable enhancement in the experimental cross-sections has been observed for {alpha}-emitting channels over the theoretical once, which may be attributed to the incomplete fusion. The percentage fraction of incomplete fusion has also been deduced, which seems to be sensitive for projectile energy, entrance channel mass-asymmetry and/or projectile structure. The present work deals with the competition of incomplete fusion with complete fusion even at {approx}3-5 MeV/nucleon for {sup 16}O + {sup 103}Rh system.

  16. Severe hemolytic disease of the premature newborn due to RH1 incompatibility: a case report

    OpenAIRE

    Uwingabiye, Jean; ZAHID, HAFID; LABRINI, FAYÇAL; EL KHAZRAJI, ABDELHAK; YAHYAOUI, ANASS; HADEF, RACHID; Messaoudi, Nezha

    2016-01-01

    We report a case of dramatic outcome of severe hemolytic disease in a newborn due to RH1 incompatibility. A newborn with A RH1 blood group was admitted in the Mohammed V Military Teaching Hospital for the problem of hydrops fetalis associated with RH1 incompatibility. The blood group of his mother, aged 31, was AB RH1-negative and that of his 37 year old father was A RH1. The mother had a history of 4 term deliveries, 3 abortions, and 1 living child. There was no prevention by anti-D immunogl...

  17. A computational mechanistic investigation of hydrogen production in water using the [Rh(III)(dmbpy)2Cl2](+)/[Ru(II)(bpy)3](2+)/ascorbic acid photocatalytic system.

    Science.gov (United States)

    Kayanuma, Megumi; Stoll, Thibaut; Daniel, Chantal; Odobel, Fabrice; Fortage, Jérôme; Deronzier, Alain; Collomb, Marie-Noëlle

    2015-04-28

    We recently reported an efficient molecular homogeneous photocatalytic system for hydrogen (H2) production in water combining [Rh(III)(dmbpy)2Cl2](+) (dmbpy = 4,4'-dimethyl-2,2'-bipyridine) as a H2 evolving catalyst, [Ru(II)(bpy)3](2+) (bpy = 2,2'-bipyridine) as a photosensitizer and ascorbic acid as a sacrificial electron donor (Chem. - Eur. J., 2013, 19, 781). Herein, the possible rhodium intermediates and mechanistic pathways for H2 production with this system were investigated at DFT/B3LYP level of theory and the most probable reaction pathways were proposed. The calculations confirmed that the initial step of the mechanism is a reductive quenching of the excited state of the Ru photosensitizer by ascorbate, affording the reduced [Ru(II)(bpy)2(bpy˙(-))](+) form, which is capable, in turn, of reducing the Rh(III) catalyst to the distorted square planar [Rh(I)(dmbpy)2](+) species. This two-electron reduction by [Ru(II)(bpy)2(bpy˙(-))](+) is sequential and occurs according to an ECEC mechanism which involves the release of one chloride after each one-electron reduction step of the Rh catalyst. The mechanism of disproportionation of the intermediate Rh(II) species, much less thermodynamically favoured, cannot be barely ruled out since it could also be favoured from a kinetic point of view. The Rh(I) catalyst reacts with H3O(+) to generate the hexa-coordinated hydride [Rh(III)(H)(dmbpy)2(X)](n+) (X = Cl(-) or H2O), as the key intermediate for H2 release. The DFT study also revealed that the real source of protons for the hydride formation as well as the subsequent step of H2 evolution is H3O(+) rather than ascorbic acid, even if the latter does govern the pH of the aqueous solution. Besides, the calculations have shown that H2 is preferentially released through an heterolytic mechanism by reaction of the Rh(III)(H) hydride and H3O(+); the homolytic pathway, involving the reaction of two Rh(III)(H) hydrides, being clearly less favoured. In parallel to this

  18. Mathematical Model of Extrinsic Blood Coagulation Cascade Dynamic System

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The blood coagulation system is very important to life. This paper presents a mathematical blood coagulation model for the extrinsic pathway. This model simulates clotting factor VIII, which plays an important role in the coagulation mechanism. The mathematical model is used to study the equilibrium stability, orbit structure, attractors and global stability behavior, with conclusions in accordance with the physiological phenomena. Moreover, the results provide information about blood related illnesses, which can be used for further study of the coagulation mechanism.

  19. RH Packaging Operations Manual

    Energy Technology Data Exchange (ETDEWEB)

    Washington TRU Solutions LLC

    2003-09-17

    This procedure provides operating instructions for the RH-TRU 72-B Road Cask, Waste Shipping Package. In this document, ''Packaging'' refers to the assembly of components necessary to ensure compliance with the packaging requirements (not loaded with a payload). ''Package'' refers to a Type B packaging that, with its radioactive contents, is designed to retain the integrity of its containment and shielding when subject to the normal conditions of transport and hypothetical accident test conditions set forth in 10 CFR Part 71. Loading of the RH 72-B cask can be done two ways, on the RH cask trailer in the vertical position or by removing the cask from the trailer and loading it in a facility designed for remote-handling (RH). Before loading the 72-B cask, loading procedures and changes to the loading procedures for the 72-B cask must be sent to CBFO at sitedocuments@wipp.ws for approval.

  20. Evaluation of Verigene Blood Culture Test Systems for Rapid Identification of Positive Blood Cultures.

    Science.gov (United States)

    Kim, Jae-Seok; Kang, Go-Eun; Kim, Han-Sung; Kim, Hyun Soo; Song, Wonkeun; Lee, Kyu Man

    2016-01-01

    The performance of molecular tests using the Verigene Gram-Positive and Gram-Negative Blood Culture nucleic acid tests (BC-GP and BC-GN, resp.; Naosphere, Northbrook, IL, USA) was evaluated for the identification of microorganisms detected from blood cultures. Ninety-nine blood cultures containing Gram-positive bacteria and 150 containing Gram-negative bacteria were analyzed using the BC-GP and BC-GN assays, respectively. Blood cultures were performed using the Bactec blood culture system (BD Diagnostic Systems, Franklin Lakes, NJ, USA) and conventional identification and antibiotic-susceptibility tests were performed using a MicroScan system (Siemens, West Sacramento, CA, USA). When a single strain of bacteria was isolated from the blood culture, Verigene assays correctly identified 97.9% (94/96) of Gram-positive bacteria and 93.8% (137/146) of Gram-negative bacteria. Resistance genes mecA and vanA were correctly detected by the BC-GP assay, while the extended-spectrum β-lactamase CTX-M and the carbapenemase OXA resistance gene were detected from 30 cases cultures by the BC-GN assay. The BC-GP and BC-GN assays showed high agreement with conventional identification and susceptibility tests. These tests are useful for rapid identification of microorganisms and the detection of clinically important resistance genes from positive Bactec blood cultures.

  1. Frequency and Distribution of Blood Groups in Blood Donors in Western Ahmedabad AND#8211; A Hospital Based Study

    Directory of Open Access Journals (Sweden)

    Patel Piyush A

    2012-04-01

    Full Text Available Background: Up till now about 400 red cells antigen have been identified. The majority are inherited by Mendelian fashion. The ABO and Rhesus (Rh blood group system are most important for blood transfusion purposes, parental testing, legal medicine and in population genetic study. Objective: This study was conducted to determine and compare the frequency of ABO and Rh blood groups in blood donors in secondary care teaching hospital at Western Ahmedabad, Gujarat, India. Materials and Methods: A retrospective study was conducted at Blood bank, GMERS Medical College, Sola, Ahmedabad over a period of seven years from 1st January 2005 to 31st December 2011. Blood group of the blood donors was determined by commercially available standard monoclonal antisera by test tube agglutination technique. Results & conclusion: Out of 5316 subjects, 5076 (95.48% were male and 240 (4.52% were female subjects. The commonest ABO blood group present was B (39.40 % followed by O (30.79 %, A (21.94 % and AB (7.86 % in blood donors; while in Rhesus system, 5053(95.05% donors were Rh-positive and 263(4.95% donors were Rh-negative. The study has a significant implication regarding the inventory management of blood bank and transfusion services for the patient admitted in our secondary care teaching hospital. [National J of Med Res 2012; 2(2.000: 202-206

  2. Bedside arterial blood gas monitoring system using fluorescent optical sensors

    Science.gov (United States)

    Bartnik, Daniel J.; Rymut, Russell A.

    1995-05-01

    We describe a bedside arterial blood gas (ABG) monitoring system which uses fluorescent optical sensors in the measurement of blood pH, PCO2 and PO2. The Point-of-Care Arterial Blood Gas Monitoring System consists of the SensiCathTM optical sensor unit manufactured by Optical Sensors Incorporated and the TramTM Critical Care Monitoring System with ABG Module manufactured by Marquette Electronics Incorporated. Current blood gas measurement techniques require a blood sample to be removed from the patient and transported to an electrochemical analyzer for analysis. The ABG system does not require removal of blood from the patient or transport of the sample. The sensor is added to the patient's existing arterial line. ABG measurements are made by drawing a small blood sample from the arterial line in sufficient quantity to ensure an undiluted sample at the sensor. Measurements of pH, PCO2 and PO2 are made within 60 seconds. The blood is then returned to the patient, the line flushed and results appear on the bedside monitor. The ABG system offers several advantages over traditional electrochemical analyzers. Since the arterial line remains closed during the blood sampling procedure the patient's risk of infection is reduced and the caregiver's exposure to blood is eliminated. The single-use, disposable sensor can be measure 100 blood samples over 72 hours after a single two-point calibration. Quality Assurance checks are also available and provide the caregiver the ability to assess system performance even after the sensor is patient attached. The ABG module integrates with an existing bedside monitoring system. This allows ABG results to appear on the same display as ECG, respiration, blood pressure, cardiac output, SpO2, and other clinical information. The small module takes up little space in the crowded intensive care unit. Performance studies compare the ABG system with an electrochemical blood gas analyzer. Study results demonstrated accurate and precise blood

  3. Development of blood extraction system designed by female mosquito's blood sampling mechanism for bio-MEMS

    Science.gov (United States)

    Tsuchiya, Kazuyoshi; Nakanishi, Naoyuki; Nakamachi, Eiji

    2005-02-01

    A compact and wearable wristwatch type Bio-MEMS such as a health monitoring system (HMS) to detect blood sugar level for diabetic patient, was newly developed. The HMS consists of (1) a indentation unit with a microneedle to generate the skin penetration force using a shape memory alloy(SMA) actuator, (2) a pumping unit using a bimorph PZT piezoelectric actuator to extract the blood and (3) a gold (Au) electrode as a biosensor immobilized GOx and attached to the gate electrode of MOSFET to detect the amount of Glucose in extracted blood. GOx was immobilized on a self assembled spacer combined with an Au electrode by the cross-link method using BSA as an additional bonding material. The device can extract blood in a few microliter through a painless microneedle with the negative pressure by deflection of the bimorph PZT piezoelectric actuator produced in the blood chamber, by the similar way the female mosquito extracts human blood with muscle motion to flex or relax. The performances of the liquid sampling ability of the pumping unit through a microneedle (3.8mm length, 100μm internal diameter) using the bimorph PZT piezoelectric microactuator were measured. The blood extraction micro device could extract human blood at the speed of 2μl/min, and it is enough volume to measure a glucose level, compared to the amount of commercial based glucose level monitor. The electrode embedded in the blood extraction device chamber could detect electrons generated by the hydrolysis of hydrogen peroxide produced by the reaction between GOx and glucose in a few microliter extracted blood, using the constant electric current measurement system of the MOSFET type hybrid biosensor. The output voltage for the glucose diluted in the chamber was increased lineally with increase of the glucose concentration.

  4. A comparative study of the protein C system in mother's blood, cord blood and amniotic fluid.

    OpenAIRE

    Ewa Zekanowska; Waldemar Uszyński; Mieczysław Uszyński; Jarosław Kuczyński; Marek Szymański

    2010-01-01

    Activated protein C (APC) is an important anticoagulant which plays a role in pathophysiology of pregnancy, e.g. in maintenance of the uteroplacental circulation and development of the fetus as well as in pathogenesis of preeclampsia. The study objective was to compare the levels of the respective components of the protein C system (protein C, PC; protein S, PS; thrombomodulin, TM) as well as thrombin activatable fibrinolysis inhibitor - TAFI in mother's blood, cord blood and amniotic fluid. ...

  5. Fluid-filled blood pressure measurement systems.

    Science.gov (United States)

    Li, J K; van Brummelen, A G; Noordergraaf, A

    1976-05-01

    The performance of catheter-manometer systems for the measurement of pulsatile pressure has been evaluated by both experimental techniques and theoretical considerations. The former approach has shown, on occasion, multiple maxima in the amplitude response. The latter has been approached in a variety of ways, ranging from extreme lumping to application of transmission line theory while employing different configurations in the system's representation. Multiple maxima have also been seen, The present paper identifies the sources of the differences found and compares the relative merits of various theoretical approaches. It introduces the compliance of the system as a figure of merit and provides a simple first-order approximation formula for evaluation of the quality of a system. Damping and impedance matching to improve the system's frequency response were studied. It was found that they were not needed in a very stiff or a very compliant system, nor should one worry about the representation of such a system.

  6. Rh血型系统研究进展%The Research Progress of Rh Blood Group System

    Institute of Scientific and Technical Information of China (English)

    卓传尚; 卓孝福; 郭永建

    2006-01-01

    Rh血型系统是最具多态性的红细胞血型系统,在临床输血、新生儿溶血病和自身免疫性溶血性贫血中具有重要意义.本文就RH基因结构、RH等位基因、Rh蛋白功能及Rh血型基因分型等近年来的研究进展简要综述.

  7. Concept design on RH maintenance of CFETR Tokamak reactor

    Energy Technology Data Exchange (ETDEWEB)

    Song, Yuntao, E-mail: songyt@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei (China); University of Science and Technology of China, Hefei (China); Wu, Songtao; Wan, Yuanxi; Li, Jiangang [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei (China); University of Science and Technology of China, Hefei (China); Ye, Minyou [University of Science and Technology of China, Hefei (China); Zheng, Jinxing; Cheng, Yong; Zhao, Wenlong; Wei, Jianghua [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei (China)

    2014-10-15

    Highlights: •We discussed the concept design of the RH maintenance system based on the main design work of the key components for CFETR. •The main design work for RH maintenance in this paper was carried out including the divertor RH system, the blanket RH system and the transfer cask system. •The technical problems encountered in the design process were discussed. •The present concept design of remote maintenance system in this paper can meet the physical and engineering requirement of CFETR. -- Abstract: CFETR which stands for Chinese Fusion Engineering Testing Reactor is a superconducting Tokamak device. The concept design on RH maintenance of CFETR has been done in the past year. It is known that, the RH maintenance is one of the most important parts for Tokamak reactor. The fusion power was designed as 50–200 MW and its duty cycle time (or burning time) was estimated as 30–50%. The center magnetic field strength on the TF magnet is 5.0 T, the maximum capacity of the volt seconds provided by center solenoid winding will be about 160 VS. The plasma current will be 10 MA and its major radius and minor radius is 5.7 m and 1.6 m respectively. All the components of CFETR which provide their basic functions must be maintained and inspected during the reactor lifetime. Thus, the remote handling (RH) maintenance system should be a key component, which must be detailedly designed during the concept design processing of CFETR, for the operation of reactor. The main design work for RH maintenance in this paper was carried out including the divertor RH system, the blanket RH system and the transfer cask system. What is more, the technical problems encountered in the design process will also be discussed.

  8. Evaluation of the quality of blood components prepared using the Reveos automated blood processing system.

    Science.gov (United States)

    Johnson, L; Winter, K M; Kwok, M; Reid, S; Marks, D C

    2013-10-01

    The Reveos automated blood processing system has been developed to combine primary and secondary processing of whole-blood units, resulting in a plasma unit, a red-blood-cell concentrate and an interim platelet unit per input. The aim of this study was to determine product specifications and in vitro quality of components produced by the Reveos system. Whole blood was processed using the Reveos system and compared with historical Reference units produced using semi-automated methods. Reveos red cells were leucoreduced and stored in SAGM at 4°C. Reveos plasma was frozen at -30°C and factor activity was assessed after thawing. Reference red cell, plasma and buffy coats were produced by top and bottom processing. Leucoreduced Reveos and Reference platelet concentrates were prepared by pooling four interim platelet units or four buffy coats, respectively, with SSP+. Processing with the Reveos system was faster (76 min) than semi-automated separation (92 min). The red cell and platelet yields were higher in the units prepared by the Reveos system. The Reference and Reveos red cell and plasma units had very similar in vitro quality parameters. The platelet concentrates were also similar in many in vitro parameters, including pH, glucose and lactate metabolism, hypotonic shock response and phosphatidylserine expression, although platelet activation markers (CD62P and cytokine levels) were higher in the Reveos units. The Reveos system can improve blood component efficiencies through reductions in processing time, whilst maintaining similar component quality. Vox Sanguinis © 2013 International Society of Blood Transfusion.

  9. Origins of gonadotropin-releasing hormone (GnRH) in vertebrates: identification of a novel GnRH in a basal vertebrate, the sea lamprey.

    Science.gov (United States)

    Kavanaugh, Scott I; Nozaki, Masumi; Sower, Stacia A

    2008-08-01

    We cloned a cDNA encoding a novel (GnRH), named lamprey GnRH-II, from the sea lamprey, a basal vertebrate. The deduced amino acid sequence of the newly identified lamprey GnRH-II is QHWSHGWFPG. The architecture of the precursor is similar to that reported for other GnRH precursors consisting of a signal peptide, decapeptide, a downstream processing site, and a GnRH-associated peptide; however, the gene for lamprey GnRH-II does not have introns in comparison with the gene organization for all other vertebrate GnRHs. Lamprey GnRH-II precursor transcript was widely expressed in a variety of tissues. In situ hybridization of the brain showed expression and localization of the transcript in the hypothalamus, medulla, and olfactory regions, whereas immunohistochemistry using a specific antiserum showed only GnRH-II cell bodies and processes in the preoptic nucleus/hypothalamus areas. Lamprey GnRH-II was shown to stimulate the hypothalamic-pituitary axis using in vivo and in vitro studies. Lamprey GnRH-II was also shown to activate the inositol phosphate signaling system in COS-7 cells transiently transfected with the lamprey GnRH receptor. These studies provide evidence for a novel lamprey GnRH that has a role as a third hypothalamic GnRH. In summary, the newly discovered lamprey GnRH-II offers a new paradigm of the origin of the vertebrate GnRH family. We hypothesize that due to a genome/gene duplication event, an ancestral gene gave rise to two lineages of GnRHs: the gnathostome GnRH and lamprey GnRH-II.

  10. Performance of Gram staining on blood cultures flagged negative by an automated blood culture system.

    Science.gov (United States)

    Peretz, A; Isakovich, N; Pastukh, N; Koifman, A; Glyatman, T; Brodsky, D

    2015-08-01

    Blood is one of the most important specimens sent to a microbiology laboratory for culture. Most blood cultures are incubated for 5-7 days, except in cases where there is a suspicion of infection caused by microorganisms that proliferate slowly, or infections expressed by a small number of bacteria in the bloodstream. Therefore, at the end of incubation, misidentification of positive cultures and false-negative results are a real possibility. The aim of this work was to perform a confirmation by Gram staining of the lack of any microorganisms in blood cultures that were identified as negative by the BACTEC™ FX system at the end of incubation. All bottles defined as negative by the BACTEC FX system were Gram-stained using an automatic device and inoculated on solid growth media. In our work, 15 cultures that were defined as negative by the BACTEC FX system at the end of the incubation were found to contain microorganisms when Gram-stained. The main characteristic of most bacteria and fungi growing in the culture bottles that were defined as negative was slow growth. This finding raises a problematic issue concerning the need to perform Gram staining of all blood cultures, which could overload the routine laboratory work, especially laboratories serving large medical centers and receiving a large number of blood cultures.

  11. Effects of carboxymethyl chitosan on the blood system of rats

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Dawei [College of Marine Life Sciences, Ocean University of China, Qingdao 266003 (China); Han, Baoqin, E-mail: baoqinh@ouc.edu.cn [College of Marine Life Sciences, Ocean University of China, Qingdao 266003 (China); Dong, Wen; Yang, Zhao; Lv, You; Liu, Wanshun [College of Marine Life Sciences, Ocean University of China, Qingdao 266003 (China)

    2011-04-29

    Highlights: {yields} We report, for the first time, the safety of carboxymethyl chitosan in blood system. {yields} CM-Chitosan has no significant effects on coagulation function of rats. {yields} CM-Chitosan has no significant effects on anticoagulation performance of rats. {yields} CM-Chitosan has no significant effects on fibrinolytic function of rats. {yields} CM-Chitosan has no significant effects on hemorheology of rats. -- Abstract: Carboxymethyl chitosan (CM-chitosan), a derivative of chitosan, was extensively studied in the biomedical materials field for its beneficial biological properties of hemostasis and stimulation of healing. However, studies examining the safety of CM-chitosan in the blood system are lacking. In this study CM-chitosan was implanted into the abdominal cavity of rats to determine blood indexes at different times and to evaluate the effects of CM-chitosan on the blood system of rats. Coagulation function was reflected by thrombin time (TT), prothrombin time (PT), activated partial thromboplatin time (APTT), fibrinogen (FIB) and platelet factor 4 (PF4) indexes; anti-coagulation performance was assessed by the index of antithrombinIII (ATIII); fibrinolytic function was reflected by plasminogen (PLG) and fibrin degradation product (FDP) indexes; and blood viscosity (BV) and plasma viscosity (PV) indexes reflected hemorheology. Results showed that CM-chitosan has no significant effects on the blood system of rats, and provides experimental basis for CM-chitosan to be applied in the field of biomedical materials.

  12. Influence of Blood Collection Systems on Coagulation Tests

    Directory of Open Access Journals (Sweden)

    Soner Yavaş

    2012-12-01

    Full Text Available OBJECTIVE: Coagulation tests are influenced by pre-analytic conditions such as blood collection systems. Change of glass collection tubes with plastic ones will cause alteration of the test results. The aim of this study was to compare three plastic blood collection tubes with a standard glass blood collection tube and each plastic collection tube with the other two for possible additional tube-to- tube differences. METHODS: A total of 284 blood samples were obtained from 42 patients receiving warfarin during their routine controls, besides 29 healthy volunteers. Subgroup analyses were done according to health status. RESULTS: Our study demonstrated that different blood collection tubes have a statistically significant influence on coagulation tests. The magnitude of the effect depends on the tube used. However most of the tests performed on samples obtained from any tube correlated significantly with results obtained from other tube samples. CONCLUSION: Although blood collection tubes with different brands or properties will have distinct effects on coagulation tests, the influence of these blood collection tubes may be relatively small to interfere with decision-making on dose prescription, therefore lack clinical importance. Correlations between the results showed that, one of these plastic blood collection tubes tested in our study, can be used interchangably for a wide variety of coagulation assays.

  13. 重组人血小板生成素和重组人白介素-11对正常猕猴全血凝血功能和血小板的影响%The Influence of rhTPO and rhIL-11 on the Platelet and Coagulability of Whole Blood in Nomal Monkeys

    Institute of Scientific and Technical Information of China (English)

    柳晓兰; 罗庆良; 熊国林; 邱丽玲; 崔玉芳

    2004-01-01

    为重组人血小板生成素(小TPO)和重组人白介素-11(rhIL-11)用于治疗血小板生成低下疾病的临床应用提供实验依据,观察给rhTPO和thIL-11后猕猴血小板数和二磷酸腺苷或凝血酶诱导的血小板聚集功能及全血凝固活性的改变.结果:①连续给2μg/(kg·d)rhTPO 8 d和20 d,rhTPO组血小板数已明显增多,但其最大聚集率与对照值和给药差别并不显著;相同时间rhTPO组血栓弹力图(TEG)r、k和r+k值均有不同程度的缩短.ma值明显增大,提示全血凝固活性增强.②120 μg/(kg·d)和1 080 μg/(kg·d)rhIL-11连续注射49 d,两剂量组血小板数均明显高于赋型剂对照组;凝血酶诱导两组血小板最大聚集率变化不显著,ADP诱导时120μg/(kg·d)组最大聚集率变化亦不明显,而1080 μg/(kg·d)组最大聚集率则明显低于对照和120μg/(kg·d)组.停药后23 d,1 080μg/(kg·d)组血小板数和其聚集功能仍未恢复正常.说明rhTPO和rhIL-11对血小板数量和功能方面的影响基本相似,而对血浆其它凝血因素的影响可能不完全相同.

  14. Followup Audit: Enterprise Blood Management System Not Ready for Full Deployment

    Science.gov (United States)

    2014-10-23

    Blood Donor Management System (donor system) and the Blood Management Blood Bank /Transfusion Service (transfusion system). The Component Acquisition...transfusion system Business Case Analysis, EBMS will enhance the DoD blood program capabilities for blood banking and transfusion services through...state that it is used to organize the management of blood bank and transfusion services. The transfusion system will interface with outside systems

  15. Immunomodulating effect of blood transfusion: is storage time important?

    DEFF Research Database (Denmark)

    Mynster, T; Dybkjoer, E; Kronborg, Gitte;

    1998-01-01

    in stimulating TNF-alpha and IL-2 release in an ex vivo assay. METHODS: Supernatants of 10 units of whole blood and 10 units of SAGM blood were collected after 1, 21 and 35 days of standard blood bank storage. Heparinized blood from 20 healthy volunteers (as 'recipients'), corresponding in ABO and Rh type...... to the stored blood, were used in a culture system with LPS and PHA as stimulators of TNF-alpha and IL-2 release. The effect of added supernatants, from either stored whole blood or SAGM blood, on cytokine release was evaluated compared to saline as control. TNF-alpha concentration was analyzed by ELISA after...

  16. RH Packaging Program Guidance

    Energy Technology Data Exchange (ETDEWEB)

    Washington TRU Solutions LLC

    2008-01-12

    The purpose of this program guidance document is to provide the technical requirements for use, operation, inspection, and maintenance of the RH-TRU 72-B Waste Shipping Package (also known as the "RH-TRU 72-B cask") and directly related components. This document complies with the requirements as specified in the RH-TRU 72-B Safety Analysis Report for Packaging (SARP), and Nuclear Regulatory Commission (NRC) Certificate of Compliance (C of C) 9212. If there is a conflict between this document and the SARP and/or C of C, the C of C shall govern. The C of C states: "...each package must be prepared for shipment and operated in accordance with the procedures described in Chapter 7.0, Operating Procedures, of the application." It further states: "...each package must be tested and maintained in accordance with the procedures described in Chapter 8.0, Acceptance Tests and Maintenance Program of the Application." Chapter 9.0 of the SARP tasks the Waste Isolation Pilot Plant (WIPP) Management and Operating (M&O) Contractor with assuring the packaging is used in accordance with the requirements of the C of C. Because the packaging is NRC-approved, users need to be familiar with Title 10 Code of Federal Regulations (CFR) §71.8, "Deliberate Misconduct." Any time a user suspects or has indications that the conditions of approval in the C of C were not met, the U.S. Department of Energy (DOE) Carlsbad Field Office (CBFO) shall be notified immediately. The CBFO will evaluate the issue and notify the NRC if required.In accordance with 10 CFR Part 71, "Packaging and Transportation of Radioactive Material," certificate holders, packaging users, and contractors or subcontractors who use, design, fabricate, test, maintain, or modify the packaging shall post copies of (1) 10 CFR Part 21, "Reporting of Defects and Noncompliance," regulations, (2) Section 206 of the Energy Reorganization Act of 1974, and (3) NRC Form 3, Notice to Employees. These documents must be posted in a

  17. 21 CFR 862.1130 - Blood volume test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Blood volume test system. 862.1130 Section 862.1130 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry Test Systems §...

  18. 21 CFR 864.9175 - Automated blood grouping and antibody test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated blood grouping and antibody test system... Manufacture Blood and Blood Products § 864.9175 Automated blood grouping and antibody test system. (a) Identification. An automated blood grouping and antibody test system is a device used to group erythrocytes...

  19. The ABO blood group system revisited: a review and update.

    Science.gov (United States)

    Storry, J R; Olsson, M L

    2009-01-01

    The antigens of the ABO system were the first to be recognized as blood groups and actually the first human genetic markers known. Their presence and the realization of naturally occurring antibodies to those antigens lacking from the cells made sense of the erratic failure of blood transfusion hitherto and opened up the possibility of a safe treatment practice in life-threatening blood loss. Although initially apparently simple, the ABO system has come to grow in complexity over the years. The mass of knowledge relating to carbohydrate chemistry, enzymology, molecular genetics, and structural and evolutionary biology is now enormous thanks to more than a century of research using ABO as a principal model. This has provided us with data to form a solid platform of evidence-based transfusion and transplantation medicine used every day in laboratories and clinics around the globe. This review aims to summarize key findings and recent progress made toward further understanding of this surprisingly polymorphic system.

  20. RhBG and RhCG, the putative ammonia transporters, are expressed in the same cells in the distal nephron.

    Science.gov (United States)

    Quentin, Fabienne; Eladari, Dominique; Cheval, Lydie; Lopez, Claude; Goossens, Dominique; Colin, Yves; Cartron, Jean-Pierre; Paillard, Michel; Chambrey, Régine

    2003-03-01

    Two nonerythroid homologs of the blood group Rh proteins, RhCG and RhBG, which share homologies with specific ammonia transporters in primitive organisms and plants, could represent members of a new family of proteins involved in ammonia transport in the mammalian kidney. Consistent with this hypothesis, the expression of RhCG was recently reported at the apical pole of all connecting tubule (CNT) cells as well as in intercalated cells of collecting duct (CD). To assess the localization along the nephron of RhBG, polyclonal antibodies against the Rh type B glycoprotein were generated. In immunoblot experiments, a specific polypeptide of Mr approximately 50 kD was detected in rat kidney cortex and in outer and inner medulla membrane fractions. Immunocytochemical studies revealed RhBG expression in distal nephron segments within the cortical labyrinth, medullary rays, and outer and inner medulla. RhBG expression was restricted to the basolateral membrane of epithelial cells. The same localization was observed in rat and mouse kidney. RT-PCR analysis on microdissected rat nephron segments confirmed that RhBG mRNAs were chiefly expressed in CNT and cortical and outer medullary CD. Double immunostaining with RhCG demonstrated that RhBG and RhCG were coexpressed in the same cells, but with a basolateral and apical localization, respectively. In conclusion, RhBG and RhCG are present in a major site of ammonia secretion in the kidney, i.e., the CNT and CD, in agreement with their putative role in ammonium transport.

  1. Highly sensitive optical sensor system for blood leakage detection

    Science.gov (United States)

    Ueda, Masahiro; Ishikawa, Kazuhiko; Jie, Chen; Sanae, Mizuno; Touma, Yasunori

    A highly sensitive method for the detection of blood leakage has been developed, and a practical sensor system for blood concentration measurement has been constructed. The present method is based on the attenuation of laser light by blood cells. The effects of the fluctuations of the incident laser light power are eliminated by normalizing the attenuated light intensity by the incident light intensity. A part of the incident laser light is reflected by a beam splitter mounted at the entrance of the test cell, of which the power is measured to provide base data for normalization. The optical path is extended to enhance sensitivity by using a pair of side mirrors. This multi-reflection method is very effective to increase sensitivity; the maximum sensitivity obtained for blood concentration is about 4 X 10 -6 by volume, which is significantly higher than that of the conventional sensors.

  2. Optimization of parameters of the SeDeM Diagram Expert System: Hausner index (IH) and relative humidity (%RH).

    Science.gov (United States)

    Suñé-Negre, Josep M; Pérez-Lozano, Pilar; Roig, Manel; Fuster, Roser; Hernández, Carmen; Ruhí, Ramon; García-Montoya, Encarna; Miñarro, Montserrat; Ticó, Josep R

    2011-10-01

    As a methodology for characterizing substances in relation to their viability in direct compression, the SeDeM Diagram Expert System may be considered an open system in terms of the number of parameters applied and the optimization of these parameters. With the experience acquired from applying the SeDeM Diagram, in this study, we propose optimizing the parameters corresponding to the Hausner index (IH) and relative humidity (%HR) in order to simplify the mathematical calculation, so that it provides reliable data that can be extrapolated. The proposed optimization does not involve a conceptual change in the parameters considered nor a significant change in the results obtained compared with the previous calculation methodology initially established for the SeDeM Diagram Expert System, which means that the conclusions obtained by applying this method are equivalent. Copyright © 2011 Elsevier B.V. All rights reserved.

  3. GnRH在性成熟高白鲑神经系统及性腺中的分布定位%An Immunocytochemical Localization of GnRH in the Nerve System and Gonad of Mature Coregonus peled

    Institute of Scientific and Technical Information of China (English)

    曹玉洁; 贾斌; 柳建新; 李志远; 张莉

    2011-01-01

    摘要:应用免疫组织化学方法,系统观察性成熟期高白鲑(Coregonus peled)神经系统及性腺中的促性腺激素释放激素( GnRH)的分布情况。结果表明,GnRH在大脑、小脑、中脑、脊髓、延髓中免疫阳性反应明显,且主要分布在神经元内。GnRH免疫阳性细胞在卵巢和精巢中均有分布,而且其阳性部位在卵巢主要分布于小生长期卵母细胞;在精巢中主要分布于间质细胞和精原细胞中。本文讨论了GnRH直接或间接参与高白鲑性腺发育成熟调节的可能性。%Immunocytochemical staining technique was used to study the expression of gonadotropin-releasing hormone (GnRH) in the nervous system and gonad of Coregonus peled. The results showed that there were GnRH immunoreactive endocrine cells in the cerebrum,cerebellum,diencephalon,medulla oblongata,and spinal cord. There were GnRH immunoreactive endocrine cells in the ovary and testis. The positive staining was observed mainly in the small growing oocyte, and in the interstitial cells and spermatogonia. The possible physiological function and morphological evidence of GnRH regulation on the development of gonad in C. Peled were discussed.

  4. RH Packaging Program Guidance

    Energy Technology Data Exchange (ETDEWEB)

    Washington TRU Solutions LLC

    2006-11-07

    The purpose of this program guidance document is to provide the technical requirements for use, operation, inspection, and maintenance of the RH-TRU 72-B Waste Shipping Package and directly related components. This document complies with the requirements as specified in the RH-TRU 72-B Safety Analysis Report for Packaging (SARP), and Nuclear Regulatory Commission (NRC) Certificate of Compliance (C of C) 9212. If there is a conflict between this document and the SARP and/or C of C, the C of C shall govern. The C of C states: "...each package must be prepared for shipment and operated in accordance with the procedures described in Chapter 7.0, Operating Procedures, of the application." It further states: "...each package must be tested and maintained in accordance with the procedures described in Chapter 8.0, Acceptance Tests and Maintenance Program of the Application." Chapter 9.0 of the SARP tasks the Waste Isolation Pilot Plant (WIPP) Management and Operating (M&O) Contractor with assuring the packaging is used in accordance with the requirements of the C of C. Because the packaging is NRC-approved, users need to be familiar with 10 Code of Federal Regulations (CFR) §71.8, "Deliberate Misconduct." Any time a user suspects or has indications that the conditions of approval in the C of C were not met, the U.S. Department of Energy (DOE) Carlsbad Field Office (CBFO) shall be notified immediately. CBFO will evaluate the issue and notify the NRC if required. In accordance with 10 CFR Part 71, "Packaging and Transportation of Radioactive Material," certificate holders, packaging users, and contractors or subcontractors who use, design, fabricate, test, maintain, or modify the packaging shall post copies of (1) 10 CFR Part 21, "Reporting of Defects and Noncompliance," regulations, (2) Section 206 of the Energy Reorganization Act of 1974, and (3) NRC Form 3, Notice to Employees. These documents must be posted in a conspicuous location where the activities subject to

  5. SBR-Blood: systems biology repository for hematopoietic cells.

    Science.gov (United States)

    Lichtenberg, Jens; Heuston, Elisabeth F; Mishra, Tejaswini; Keller, Cheryl A; Hardison, Ross C; Bodine, David M

    2016-01-04

    Extensive research into hematopoiesis (the development of blood cells) over several decades has generated large sets of expression and epigenetic profiles in multiple human and mouse blood cell types. However, there is no single location to analyze how gene regulatory processes lead to different mature blood cells. We have developed a new database framework called hematopoietic Systems Biology Repository (SBR-Blood), available online at http://sbrblood.nhgri.nih.gov, which allows user-initiated analyses for cell type correlations or gene-specific behavior during differentiation using publicly available datasets for array- and sequencing-based platforms from mouse hematopoietic cells. SBR-Blood organizes information by both cell identity and by hematopoietic lineage. The validity and usability of SBR-Blood has been established through the reproduction of workflows relevant to expression data, DNA methylation, histone modifications and transcription factor occupancy profiles. Published by Oxford University Press on behalf of Nucleic Acids Research 2015. This work is written by (a) US Government employee(s) and is in the public domain in the US.

  6. Determination of the dominant catalyst derived from the classic [RhCp*Cl₂]₂ precatalyst system: Is it single-metal Rh₁Cp*-based, subnanometer Rh₄ cluster-based, or Rh(0)n nanoparticle-based cyclohexene hydrogenation catalysis at room temperature and mild pressures?

    Energy Technology Data Exchange (ETDEWEB)

    Bayram, Ercan [Colorado State Univ., Fort Collins, CO (United States); Linehan, John C. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Fulton, John L. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Szymczak, Nathaniel K. [Univ. of Michigan, Ann Arbor, MI (United States); Finke, Richard G. [Colorado State Univ., Fort Collins, CO (United States)

    2015-05-27

    Determining the kinetically dominant catalyst in a given catalytic system is a forefront topic in catalysis. The [RhCp*Cl₂]₂ (Cp* =[η⁵-C₅(CH₃)₅]) system pioneered by Maitlis and co-workers is a classic precatalyst system from which homogeneous mononuclear Rh₁, subnanometer Rh₄ cluster, and heterogeneous polymetallic Rh(0)n nanoparticle have all arisen as viable candidates for the true hydrogenation catalyst, depending on the precise substrate, H₂ pressure, temperature, and catalyst concentration conditions. Addressed herein is the question of whether the prior assignment of homogeneous, mononuclear Rh₁Cp*-based catalysis is correct, or are trace Rh₄ subnanometer clusters or possibly Rh(0)n nanoparticles the dominant, actual cyclohexene hydrogenation catalyst at 22 °C and 2.7 atm initial H₂ pressure? The observation herein of Rh₄ species by in operando-X-ray absorption fine structure (XAFS) spectroscopy, at the only slightly more vigorous conditions of 26 °C and 8.3 atm H₂ pressure, and the confirmation of Rh₄ clusters by ex situ mass spectroscopy raises the question of the dominant, room temperature, and mild pressure cyclohexene hydrogenation catalyst derived from the classic [RhCp*Cl₂]₂ precatalyst pioneered by Maitlis and co-workers. Ten lines of evidence are provided herein to address the nature of the true room temperature and mild pressure cyclohexene hydrogenation catalyst derived from [RhCp*Cl₂]₂. Especially significant among those experiments are quantitative catalyst poisoning experiments, in the present case using 1,10-phenanthroline. Those poisoning studies allow one to distinguish mononuclear Rh₁, subnanometer Rh₄ cluster, and Rh(0)n nanoparticle catalysis hypotheses. The evidence obtained provides a compelling case for a mononuclear, Rh₁Cp*-based cyclohexene hydrogenation catalyst at 22 °C and 2.7 atm H₂ pressure. The resultant methodology, especially the quantitative

  7. Analysis on the Application of ERP System in the Operations Management of RH Supermarket in Lianshui%基于ERP系统在涟水RH超市运营管理中的应用分析

    Institute of Scientific and Technical Information of China (English)

    王晶晶; 汪磊

    2015-01-01

    ERP system is one of the most advanced means of the informatization management of the company. It realizes the management the whole supply chain and information system of the company. The implementation of ERP system is a very difficult and pressing task. Through the research of Lianshui RH supermarket, this paper analyzes the construction situation and existing problems of ERP system and puts forward the measures of solving the application problems of ERP to further improve the efficiency of ERP in RH supermarket, and then accelerate the development of RH supermarket.%ERP系统是企业信息化管理最先进的手段之一,它实现了对企业整个供应链和整个信息系统的管理。实施ERP系统是一项非常艰巨而棘手的任务。本文通过对涟水RH超市调研,分析ERP系统建设现状及存在问题,并提出解决ERP应用问题的措施,以此进一步提高RH超市的ERP运行效率,进而促进RH超市快速发展。

  8. Microfluidic filtration system to isolate extracellular vesicles from blood.

    Science.gov (United States)

    Davies, Ryan T; Kim, Junho; Jang, Su Chul; Choi, Eun-Jeong; Gho, Yong Song; Park, Jaesung

    2012-12-21

    Extracellular vesicles are released by various cell types, particularly tumor cells, and may be potential targets for blood-based cancer diagnosis. However, studies performed on blood-borne vesicles to date have been limited by lack of effective, standardized purification strategies. Using in situ prepared nanoporous membranes, we present a simple strategy employing a microfluidic filtration system to isolate vesicles from whole blood samples. This method can be applied to purify nano-sized particles from blood allowing isolation of intact extracellular vesicles, avoiding the need for laborious and potentially damaging centrifugation steps or overly specific antibody-based affinity purification. Porous polymer monoliths were integrated as membranes into poly(methyl methacrylate) microfluidic chips by benchtop UV photopolymerization through a mask, allowing precise positioning of membrane elements while preserving simplicity of device preparation. Pore size could be manipulated by changing the ratio of porogenic solvent to prepolymer solution, and was tuned to a size proper for extraction of vesicles. Using the membrane as a size exclusion filter, we separated vesicles from cells and large debris by injecting whole blood under pressure through the microfluidic device. To enhance isolation purity, DC electrophoresis was employed as an alternative driving force to propel particles across the filter and increase the separation efficiency of vesicles from proteins. From the whole blood of melanoma-grown mice, we isolated extracellular vesicles and performed RT-PCR to verify their contents of RNA. Melan A mRNA derived from melanoma tumor cells were found enriched in filtered samples, confirming the recovery of vesicles via their cargo. This filtration system can be incorporated into other on-chip processes enabling integrated sample preparation for the downstream analysis of blood-based extracellular vesicles.

  9. RH Packaging Program Guidance

    Energy Technology Data Exchange (ETDEWEB)

    Washington TRU Solutions, LLC

    2003-08-25

    The purpose of this program guidance document is to provide technical requirements for use, operation, inspection, and maintenance of the RH-TRU 72-B Waste Shipping Package and directly related components. This document complies with the requirements as specified in the RH-TRU 72-B Safety Analysis Report for Packaging (SARP), and Nuclear Regulatory Commission (NRC) Certificate of Compliance (C of C) 9212. If there is a conflict between this document and the SARP and/or C of C, the SARP and/or C of C shall govern. The C of C states: ''...each package must be prepared for shipment and operated in accordance with the procedures described in Chapter 7.0, ''Operating Procedures,'' of the application.'' It further states: ''...each package must be tested and maintained in accordance with the procedures described in Chapter 8.0, ''Acceptance Tests and Maintenance Program of the Application.'' Chapter 9.0 of the SARP tasks the Waste Isolation Pilot Plant (WIPP) Management and Operating (M&O) contractor with assuring the packaging is used in accordance with the requirements of the C of C. Because the packaging is NRC approved, users need to be familiar with 10 CFR {section} 71.11, ''Deliberate Misconduct.'' Any time a user suspects or has indications that the conditions of approval in the C of C were not met, the Carlsbad Field Office (CBFO) shall be notified immediately. CBFO will evaluate the issue and notify the NRC if required. This document details the instructions to be followed to operate, maintain, and test the RH-TRU 72-B packaging. This Program Guidance standardizes instructions for all users. Users shall follow these instructions. Following these instructions assures that operations are safe and meet the requirements of the SARP. This document is available on the Internet at: ttp://www.ws/library/t2omi/t2omi.htm. Users are responsible for ensuring they are using the current

  10. A tauberian approach to RH

    CERN Document Server

    Cloitre, Benoit

    2011-01-01

    The aim of this paper is twofold. Firstly we present our main discovery arising from experiments which is the tauberian concept of functions of good variation (FGV). Secondly we propose to use these FGV for proving RH is true via some conjectures. More precisely we give an implicit definition of FGV and we provide several smooth and nontrivial exemples from experiments. Then using a conjectured family of FGV involving functions approaching the floor function we derive RH is true. We make also a tauberian conjecture allowing us to prove RH is true for L-functions (the generalized RH) and automorphic L-functions (the grand RH) and we discuss the linear independance conjecture. The method is inspired by the Ingham summation process and the experimental support is provided using pari-gp.

  11. Manufacturing routes for disposable polymer blood diagnostic microfluidic systems

    DEFF Research Database (Denmark)

    Tosello, Guido; Griffiths, Christian; Azcarate, Sabino

    2008-01-01

    The future vision of multi - analysis point of care testing (POCT) shows a hand-held device that patients can use with an ease similar to current blood sugar test systems. Additionally the mobile instrument would require transfer of the measured test results wirelessly to the doctor’s office, thus...

  12. [Possibility of using DST-30 thermoelastoplast in blood transfusion systems].

    Science.gov (United States)

    Il'iakov, E V; El'tsefon, B S; Polezhaev, V V; Zotkina, I V; Krivonosov, A I

    1979-01-01

    Investigating possible application of the DCT-30 thermoelastoplastic shows advantages of some physico-technical properties of this product based on natural caoutchouc and latex by comparison with base-rubber onene. It justifies recommending the former for making injection tubes in the blood transfusion systems.

  13. Variability in blood and blood component utilization as assessed by an anesthesia information management system.

    Science.gov (United States)

    Frank, Steven M; Savage, Will J; Rothschild, Jim A; Rivers, Richard J; Ness, Paul M; Paul, Sharon L; Ulatowski, John A

    2012-07-01

    Data can be collected for various purposes with anesthesia information management systems. The authors describe methods for using data acquired from an anesthesia information management system to assess intraoperative utilization of blood and blood components. Over an 18-month period, data were collected on 48,086 surgical patients at a tertiary care academic medical center. All data were acquired with an automated anesthesia recordkeeping system. Detailed reports were generated for blood and blood component utilization according to surgical service and surgical procedure, and for individual surgeons and anesthesiologists. Transfusion hemoglobin trigger and target concentrations were compared among surgical services and procedures, and between individual medical providers. For all patients given erythrocytes, the mean transfusion hemoglobin trigger was 8.4 ± 1.5, and the target was 10.2 ± 1.5 g/dl. Variation was significant among surgical services (trigger range: 7.5 ± 1.2-9.5 ± 1.1, P = 0.0001; target range: 9.1 ± 1.2-11.3 ± 1.4 g/dl, P = 0.002), surgeons (trigger range: 7.2 ± 0.7-9.8 ± 1.0, P = 0.001; target range: 8.8 ± 0.9-11.8 ± 1.3 g/dl, P = 0.001), and anesthesiologists (trigger range: 7.2 ± 0.8-9.6 ± 1.2, P = 0.001; target range: 9.0 ± 0.9-11.7 ± 1.3 g/dl, P = 0.0004). The use of erythrocyte salvage, fresh frozen plasma, and platelets varied threefold to fourfold among individual surgeons compared with their peers performing the same surgical procedure. The use of data acquired from an anesthesia information management system allowed a detailed analysis of blood component utilization, which revealed significant variation among surgical services and surgical procedures, and among individual anesthesiologists and surgeons compared with their peers. Incorporating these methods of data acquisition and analysis into a blood management program could reduce unnecessary transfusions, an outcome that may increase patient safety and reduce costs.

  14. Catalytic activity and mechanistic approach of NO reduction by CO over M0.05Co2.95O4 (M = Rh, Pd & Ru) spinel system

    Science.gov (United States)

    Salker, A. V.; Desai, M. S. Fal

    2016-12-01

    Pd, Rh and Ru doped cobalt oxide spinels have been prepared by citric acid assisted sol-gel method to yield nano-catalysts. Compositions have been characterized by XRD, FTIR, TG/DTA, BET, SEM, TEM, TPD and XPS techniques. Simultaneous catalytic detoxification of nitric oxide and carbon monoxide is investigated over the doped and pristine catalysts. Metal doping is found to enhance the activity of the catalysts due to their better adsorption capacity. Moisture and oxygen tolerance is investigated for Rh and Pd substituted catalysts. A probable reaction mechanism on the catalyst surface has been proposed.

  15. How the python heart separates pulmonary and systemic blood pressures and blood flows.

    Science.gov (United States)

    Jensen, Bjarke; Nielsen, Jan M; Axelsson, Michael; Pedersen, Michael; Löfman, Carl; Wang, Tobias

    2010-05-01

    The multiple convergent evolution of high systemic blood pressure among terrestrial vertebrates has always been accompanied by lowered pulmonary pressure. In mammals, birds and crocodilians, this cardiac separation of pressures relies on the complete division of the right and left ventricles by a complete ventricular septum. However, the anatomy of the ventricle of most reptiles does not allow for complete anatomical division, but the hearts of pythons and varanid lizards can produce high systemic blood pressure while keeping the pulmonary blood pressure low. It is also known that these two groups of reptiles are characterised by low magnitudes of cardiac shunts. Little, however, is known about the mechanisms that allow for this pressure separation. Here we provide a description of cardiac structures and intracardiac events that have been revealed by ultrasonic measurements and angioscopy. Echocardiography revealed that the atrioventricular valves descend deep into the ventricle during ventricular filling and thereby greatly reduce the communication between the systemic (cavum arteriosum) and pulmonary (cavum pulmonale) ventricular chambers during diastole. Angioscopy and echocardiography showed how the two incomplete septa, the muscular ridge and the bulbuslamelle - ventricular structures common to all squamates - contract against each other in systole and provide functional division of the anatomically subdivided ventricle. Washout shunts are inevitable in the subdivided snake ventricle, but we show that the site of shunting, the cavum venosum, is very small throughout the cardiac cycle. It is concluded that the python ventricle is incapable of the pronounced and variable shunts of other snakes, because of its architecture and valvular mechanics.

  16. Cell saver filtering of extravasated rhBMP-2 after degenerative scoliosis reconstruction

    Directory of Open Access Journals (Sweden)

    Gabriel Liu, MBBCh, MSc, FRCS, FAMS (Orth

    2015-06-01

    Full Text Available RhBMP-2 is a bone fusion enhancer commonly used in scoliosis reconstruction surgery. It is delivered via an absorbable collagen sponge but has been known to migrate away from its delivery site. RhBMP-2 extravasation in surgical drainage has been noted during first two days post-surgery. Cell savers are widely used in scoliosis reconstruction to limit transfusion requirements and are commonly deployed in cases where rhBMP-2 is used for fusion augmentation. It is not known whether rhBMP-2 is present in salvaged blood or filtered away during cell saver recycling. Through this case series of four patients who underwent scoliosis reconstruction, we assess cell saver efficacy in filtering rhBMP-2 molecules by quantifying the amount of rhBMP-2 present in salvaged blood obtained after postoperative drainage recycling by OrthoPAT® cell saver and comparing it to rhBMP-2 leakage in postoperative drainage without cell saver recycling. We report an almost 10-fold reduction of rhBMP-2 concentration in salvaged blood obtained after cell saver recycling of postoperative drainage, suggesting cell saver effectiveness in filtering rhBMP-2 molecules.

  17. New approach to 'top-and-bottom' whole blood separation using the multiunit TACSI WB system: quality of blood components.

    Science.gov (United States)

    Lotens, A; Najdovski, T; Cellier, N; Ernotte, B; Lambermont, M; Rapaille, A

    2014-10-01

    TACSI whole blood system is designed to combine primary and secondary processing of six whole blood bags into plasma units, buffy coat and red blood cell concentrates. The aim of this study was to investigate the specifications and in vitro storage parameters of blood components compared with standard centrifugation and separation processing. Whole blood bags, collected in CRC kits, were treated on a TACSI whole blood system. They were compared with whole blood bags collected in Composelect kits. In addition to routine quality control analyses, conservation studies were performed on red blood cell concentrates for 42 days and on plasma for 6 months. Platelets pools with five buffy coats were also created, and cellular contamination was evaluated. Red blood cell concentrates produced from TACSI whole blood met European quality requirements. For white blood cell count, one individual result exceeded 1 × 10(6) cells/unit. All plasma units fell within specifications for residual cellular contamination and storage parameters. The performances of the TACSI whole blood system allow for the preparation of low volume buffy coats with a recovery of 90% of whole blood platelets. Haemoglobin losses in TACSI BC are smaller, but this did not result in higher haemoglobin content of red cells. These BC are suitable for the production of platelet concentrates. From these in vitro data, red blood cell concentrates produced using TACSI whole blood are suitable for clinical use with a quality at least equivalent to the control group. © 2014 International Society of Blood Transfusion.

  18. Blood-Pressure Measuring System Gives Accurate Graphic Output

    Science.gov (United States)

    1965-01-01

    The problem: To develop an instrument that will provide an external (indirect) measurement of arterial blood pressure in the form of an easily interpreted graphic trace that can be correlated with standard clinical blood-pressure measurements. From sphygmograms produced by conventional sphygmographs, it is very difficult to differentiate the systolic and diastolic blood-pressure pulses and to correlate these indices with the standard clinical values. It is nearly impossible to determine these indices when the subject is under physical or emotional stress. The solution: An electronic blood-pressure system, basically similar to conventional ausculatory sphygmomanometers, employing a standard occluding cuff, a gas-pressure source, and a gas-pressure regulator and valve. An electrical output transducer senses cuff pressure, and a microphone positioned on the brachial artery under the occluding cuff monitors the Korotkoff sounds from this artery. The output signals present the conventional systolic and diastolic indices in a clear, graphical display. The complete system also includes an electronic timer and cycle-control circuit.

  19. Influence of aging time on thermodynamical, structural and microstructural properties of Pd{sub 100-(x+y)}Pt{sub x}Rh{sub y-}T{sub 2}(D{sub 2}) systems; Influence de la duree de stockage sur les proprietes thermodynamiques, structurales et microstructurales des systemes Pd{sub 100-(x+y)}Pt{sub x}Rh{sub y-}T{sub 2}(D{sub 2})

    Energy Technology Data Exchange (ETDEWEB)

    Thiebaut, S

    1998-12-31

    This study has demonstrated that substitution of palladium by a few atomic percent of platinum and/or rhodium induces great changes in tritium storage properties and aging phenomena in these systems. Before aging, equilibrium pressures only depend of the global substitution rate, and not at all of the substituents nature. During storage, the decrease of plateau pressures is stronger in Pd-Pt alloys, and even more in the ternary solid solution Pd{sub 90}Pt{sub 5}Rh{sub 5}. This substitution effect was explained by a difference in the nature of defects created by {sup 3}He bubbles formation. The presence of platinum, with or without rhodium, in the lattice stabilizes self interstitial atoms isolated or in dislocation loops. These defects induce a great lattice swelling, and the tritides stability is directly proportional to the metal lattice volume. On the contrary, in pure palladium and Pd-Rh alloys, self interstitial atoms are preferentially integrated in a dislocation network, so they are without effect on the lattice parameter and consequently on the tritides stability. In all cases, Pd-Pt-Rh systems are indeed perfectly suited for tritium storage thanks to their conservation of local order and thermodynamical properties during aging. (author) 81 refs.

  20. The effect of Panax notoginseng on blood coagulation system

    OpenAIRE

    橋本, 洋幸; 荒, 敏昭; 藤波, 義明; 服部, 敏己; 王, 宝禮; 宮沢, 裕夫

    2011-01-01

    Panax notoginseng is used as a hemostatic drug to treat the dental extraction socket. However, the mechanisms remain to be elucidated. Therefore, the effects of Panax notoginseng on the blood coagulation system were examined in this study. Extraction of Panax notoginseng with phosphate-buffered saline (PBS) or PBS + bovine serum albumin (BSA) prolonged activated partial thromboplastin time (APTT), but had no effect on prothrombin time (PT). APTT by treatment with PBS+BSA-extraction was shorte...

  1. 78 FR 18353 - Guidance for Industry: Blood Establishment Computer System Validation in the User's Facility...

    Science.gov (United States)

    2013-03-26

    ... HUMAN SERVICES Food and Drug Administration Guidance for Industry: Blood Establishment Computer System... ``Guidance for Industry: Blood Establishment Computer System Validation in the User's Facility'' dated April... establishment computer system validation program, consistent with recognized principles of software...

  2. Hydroformylation of 1-Hexene over Rh/Nano-Oxide Catalysts

    Directory of Open Access Journals (Sweden)

    Sari Suvanto

    2013-03-01

    Full Text Available The effect of nanostructured supports on the activity of Rh catalysts was studied by comparing the catalytic performance of nano- and bulk-oxide supported Rh/ZnO, Rh/SiO2 and Rh/TiO2 systems in 1-hexene hydroformylation. The highest activity with 100% total conversion and 96% yield of aldehydes was obtained with the Rh/nano-ZnO catalyst. The Rh/nano-ZnO catalyst was found to be more stable and active than the corresponding rhodium catalyst supported on bulk ZnO. The favorable morphology of Rh/nano-ZnO particles led to an increased metal content and an increased number of weak acid sites compared to the bulk ZnO supported catalysts. Both these factors favored the improved catalytic performance. Improvements of catalytic properties were obtained also with the nano-SiO2 and nano-TiO2 supports in comparison with the bulk supports. All of the catalysts were characterized by scanning electron microscope (SEM, inductively coupled plasma mass spectrometry (ICP-MS, BET, powder X-ray diffraction (PXRD and NH3- temperature-programmed desorption (TPD.

  3. The LAN blood group system:a review.

    Science.gov (United States)

    Peyrard, Thierry

    2013-01-01

    LAN (Langereis) was officially recognized by the International Society of Blood Transfusion in 2012 as being the 33rd human blood group system. It consists of one single high-prevalence antigen,Lan (LANl). The ABCB6 protein is the carrier of the Lan blood group antigen. The ABCB6 gene (chromosome 2q36, 19 exons)encodes the ABCB6 polypeptide (ATP-binding cassette protein,subfamily B, member 6), known as a porphyrin transporter.The exceptional Lan- people do not express ABCB6 (Lan null phenotype), owing to several different molecular mechanisms affecting ABCB6: frameshift leading to a premature stop codon(deletion, insertion, or nonsense mutation of nucleotides);missense mutation; or intronic mutation responsible for RNA splicing defect. Despite the Lan antigen's being reported to play a key role in erythropoiesis and detoxification of cells, Lan people do not appear to demonstrate susceptibility to any disease or seemingly physiologic disorder. Anti-Lan has been described as having variable clinical significance, either for hemolytic transfusion reactions (none to severe) or hemolytic disease of the fetus and newborn (none to mild). Despite challenging conditions caused by the scarcity of Lan- donors worldwide, Lan- blood should ideally be given to patients with anti-Lan, especially those with a high-titer antibody.

  4. Hygroscopicity of internally mixed particles composed of (NH4)2SO4 and citric acid under pulsed RH change.

    Science.gov (United States)

    Shi, Xiao-Min; Wu, Feng-Min; Jing, Bo; Wang, Na; Xu, Lin-Lin; Pang, Shu-Feng; Zhang, Yun-Hong

    2017-09-09

    In this research, we applied a pulsed RH controlling system and a rapid scan vacuum FTIR spectrometer (PRHCS-RSVFTIR) to investigate hygroscopicity of internally mixed (NH4)2SO4(AS)/citric acid (CA) particles. The water content and efflorescence ratio of AS in the particles and ambient relative humidity (RH) as a function of time were obtained with a subsecond time resolution. The hygroscopic behavior of AS aerosols in two different RH control processes (equilibrium and RH pulsed processes) showed that AS droplets crystallize with RH ranging from 42% to 26.5%. It was found that the half-life time ratio between the water content in the CA particles and the gas phase under RH pulsed change was greater than one under low RH conditions (RH), indicating the significant water transfer limitation due to the high viscosity of CA aerosols at low RH, especially at RHRH in atmosphere. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Determination of ABO blood grouping and Rhesus factor from tooth material

    Science.gov (United States)

    Kumar, Pooja Vijay; Vanishree, M; Anila, K; Hunasgi, Santosh; Suryadevra, Sri Sujan; Kardalkar, Swetha

    2016-01-01

    Objective: The aim of the study was to determine blood groups and Rhesus factor from dentin and pulp using absorption-elution (AE) technique in different time periods at 0, 3, 6, 9 and 12 months, respectively. Materials and Methods: A total of 150 cases, 30 patients each at 0, 3, 6, 9 and 12 months were included in the study. The samples consisted of males and females with age ranging 13–60 years. Patient's blood group was checked and was considered as “control.” The dentin and pulp of extracted teeth were tested for the presence of ABO/Rh antigen, at respective time periods by AE technique. Statistical Analysis: Data were analyzed in proportion. For comparison, Chi-square test or Fisher's exact test was used for the small sample. Results: Blood group antigens of ABO and Rh factor were detected in dentin and pulp up to 12 months. For both ABO and Rh factor, dentin and pulp showed 100% sensitivity for the samples tested at 0 month and showed a gradual decrease in the sensitivity as time period increased. The sensitivity of pulp was better than dentin for both the blood grouping systems and ABO blood group antigens were better detected than Rh antigens. Conclusion: In dentin and pulp, the antigens of ABO and Rh factor were detected up to 12 months but showed a progressive decrease in the antigenicity as the time period increased. When compared the results obtained of dentin and pulp in ABO and Rh factor grouping showed similar results with no statistical significance. The sensitivity of ABO blood grouping was better than Rh factor blood grouping and showed a statistically significant result. PMID:27721625

  6. Determination of ABO blood grouping and Rhesus factor from tooth material.

    Science.gov (United States)

    Kumar, Pooja Vijay; Vanishree, M; Anila, K; Hunasgi, Santosh; Suryadevra, Sri Sujan; Kardalkar, Swetha

    2016-01-01

    The aim of the study was to determine blood groups and Rhesus factor from dentin and pulp using absorption-elution (AE) technique in different time periods at 0, 3, 6, 9 and 12 months, respectively. A total of 150 cases, 30 patients each at 0, 3, 6, 9 and 12 months were included in the study. The samples consisted of males and females with age ranging 13-60 years. Patient's blood group was checked and was considered as "control." The dentin and pulp of extracted teeth were tested for the presence of ABO/Rh antigen, at respective time periods by AE technique. Data were analyzed in proportion. For comparison, Chi-square test or Fisher's exact test was used for the small sample. Blood group antigens of ABO and Rh factor were detected in dentin and pulp up to 12 months. For both ABO and Rh factor, dentin and pulp showed 100% sensitivity for the samples tested at 0 month and showed a gradual decrease in the sensitivity as time period increased. The sensitivity of pulp was better than dentin for both the blood grouping systems and ABO blood group antigens were better detected than Rh antigens. In dentin and pulp, the antigens of ABO and Rh factor were detected up to 12 months but showed a progressive decrease in the antigenicity as the time period increased. When compared the results obtained of dentin and pulp in ABO and Rh factor grouping showed similar results with no statistical significance. The sensitivity of ABO blood grouping was better than Rh factor blood grouping and showed a statistically significant result.

  7. Miniaturized Blood Pressure Telemetry System with RFID Interface

    Directory of Open Access Journals (Sweden)

    Michele Caldara

    2016-08-01

    Full Text Available This work deals with the development and characterization of a potentially implantable blood pressure telemetry system, based on an active Radio-Frequency IDentification (RFID tag, International Organization for Standardization (ISO 15693 compliant. This approach aims to continuously measure the average, systolic and diastolic blood pressure of the small/medium animals. The measured pressure wave undergoes embedded processing and results are stored onboard in a non-volatile memory, providing the data under interrogation by an external RFID reader. In order to extend battery lifetime, RFID energy harvesting has been investigated. The paper presents the experimental characterization in a laboratory and preliminary in-vivo tests. The device is a prototype mainly intended, in a future engineered version, for monitoring freely moving test animals for pharmaceutical research and drug safety assessment purposes, but it could have multiple uses in environmental and industrial applications.

  8. Probing the interaction of Rh, Co and bimetallic Rh-Co nanoparticles with the CeO2 support: catalytic materials for alternative energy generation.

    Science.gov (United States)

    Varga, E; Pusztai, P; Óvári, L; Oszkó, A; Erdőhelyi, A; Papp, C; Steinrück, H-P; Kónya, Z; Kiss, J

    2015-10-28

    The interaction of CeO2-supported Rh, Co and bimetallic Rh-Co nanoparticles, which are active catalysts in hydrogen production via steam reforming of ethanol, a process related to renewable energy generation, was studied by X-ray diffraction (XRD), high resolution electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and low energy ion scattering (LEIS). Furthermore, diffuse reflectance infrared spectroscopy (DRIFTS) of adsorbed CO as a probe molecule was used to characterize the morphology of metal particles. At small loadings (0.1%), Rh is in a much dispersed state on ceria, while at higher contents (1-5%), Rh forms 2-8 nm particles. Between 473-673 K pronounced oxygen transfer from ceria to Rh is observed and at 773 K significant agglomeration of Rh occurs. On reduced ceria, XPS indicates a possible electron transfer from Rh to ceria. The formation of smaller ceria crystallites upon loading with Co was concluded from XRD and HRTEM; for 10% Co, the CeO2 particle size decreased from 27.6 to 10.7 nm. A strong dissolution of Co into ceria and a certain extent of encapsulation by ceria were deduced by XRD, XPS and LEIS. In the bimetallic system, the presence of Rh enhances the reduction of cobalt and ceria. During thermal treatments, reoxidation of Co occurs, and Rh agglomeration as well as oxygen migration from ceria to Rh are hindered in the presence of cobalt.

  9. The role of GABA in the regulation of GnRH neurons

    Directory of Open Access Journals (Sweden)

    Miho eWatanabe

    2014-11-01

    Full Text Available Gonadotropin-releasing hormone (GnRH neurons form the final common pathway for the central regulation of reproduction. Gamma-amino butyric acid (GABA has long been implicated as one of the major players in the regulation of GnRH neurons. Although GABA is typically an inhibitory neurotransmitter in the mature adult central nervous system, most mature GnRH neurons show the unusual characteristic of being excited by GABA. While many reports have provided much insight into the contribution of GABA to the activity of GnRH neurons, the precise physiological role of the excitatory action of GABA on GnRH neurons remains elusive. This brief review presents the current knowledge of the role of GABA signaling in GnRH neuronal activity. We also discuss the modulation of GABA signaling by neurotransmitters and neuromodulators and the functional consequence of GABAergic inputs to GnRH neurons in both the physiology and pathology of reproduction.

  10. Poor performance of BACTEC NR 730 blood culture system in early detection of Neisseria meningitidis.

    Science.gov (United States)

    Schnur, E R; Azimi, P H; Belchis, D A

    1989-04-01

    During an 8-month period at Children's Hospital, Oakland, Calif., a 9% rate for positive blood culture for children with Neisseria meningitidis meningitis was identified. The blood culture system used in each case was the BACTEC NR 730. This rate seemed significantly lower than previous rates (33 to 55%) (P.R. Dodge and M.N. Swartz, N. Engl. J. Med. 272:1003-1010, 1965; A.L. Hoyne and R.H. Brown, Ann. Intern. Med. 28:248-259, 1948; S. Levin and M.B. Painter, Ann. Intern. Med. 64:1049-1057, 1966). The low rate prompted our study. With 14 test strains, anaerobic and aerobic BACTEC bottles were evaluated for their ability to support and detect the growth of N. meningitidis. Sodium polyanetholesufonate (SPS) and inoculum size, two factors thought to affect the growth of N. meningitidis, were controlled for by use of bottles with and without SPS and by inoculum sizes simulating the magnitudes of bacteremia previously described for children infected with N. meningitidis (L.J. La Scolea, Jr., D. Dryja, T.D. Sullivan, L. Mosovich, N. Ellerstein, and E. Neter, J. Clin. Microbiol. 13:478-482, 1981). BACTEC failed to detect growth in aerobic bottles after 6 h of incubation, while 76 of 80 bottles (95%) showed growth when subcultured. At 24 h, BACTEC detected growth in only 29 of 80 bottles (36%); when subcultured, all 80 cultures grew confluently. At 48 h, BACTEC detected growth in the remaining 53 bottles. BACTEC failed to detect growth in anaerobic bottles at 6 h and at 1, 2, 4, and 5 days of incubation despite growth in subculture. Subcultures from bottles with tryptic soy broth with and without SPS showed growth in 63 to 76 bottles in 6 h and in all bottles after 24 h. The presence of SPS in BACTEC bottles had no effect on growth detection. On the basis of these studies and our clinical experience, we find the NR 730 system to be insensitive and unsuitable for detection of N.meningitidis in

  11. Part I: A Comparative Thermal Aging Study on the Regenerability of Rh/Al2O3 and Rh/CexOy-ZrO2 as Model Catalysts for Automotive Three Way Catalysts

    Directory of Open Access Journals (Sweden)

    Qinghe Zheng

    2015-10-01

    Full Text Available The rhodium (Rh component in automotive three way catalysts (TWC experiences severe thermal deactivation during fuel shutoff, an engine mode (e.g., at downhill coasting used for enhancing fuel economy. In a subsequent switch to a slightly fuel rich condition, in situ catalyst regeneration is accomplished by reduction with H2 generated through steam reforming catalyzed by Rh0 sites. The present work reports the effects of the two processes on the activity and properties of 0.5% Rh/Al2O3 and 0.5% Rh/CexOy-ZrO2 (CZO as model catalysts for Rh-TWC. A very brief introduction of three way catalysts and system considerations is also given. During simulated fuel shutoff, catalyst deactivation is accelerated with increasing aging temperature from 800 °C to 1050 °C. Rh on a CZO support experiences less deactivation and faster regeneration than Rh on Al2O3. Catalyst characterization techniques including BET surface area, CO chemisorption, TPR, and XPS measurements were applied to examine the roles of metal-support interactions in each catalyst system. For Rh/Al2O3, strong metal-support interactions with the formation of stable rhodium aluminate (Rh(AlO2y complex dominates in fuel shutoff, leading to more difficult catalyst regeneration. For Rh/CZO, Rh sites were partially oxidized to Rh2O3 and were relatively easy to be reduced to active Rh0 during regeneration.

  12. The Rheology of Blood Flow in a Branched Arterial System.

    Science.gov (United States)

    Shibeshi, Shewaferaw S; Collins, William E

    2005-01-01

    Blood flow rheology is a complex phenomenon. Presently there is no universally agreed upon model to represent the viscous property of blood. However, under the general classification of non-Newtonian models that simulate blood behavior to different degrees of accuracy, there are many variants. The power law, Casson and Carreau models are popular non-Newtonian models and affect hemodynamics quantities under many conditions. In this study, the finite volume method is used to investigate hemodynamics predictions of each of the models. To implement the finite volume method, the computational fluid dynamics software Fluent 6.1 is used. In this numerical study the different hemorheological models are found to predict different results of hemodynamics variables which are known to impact the genesis of atherosclerosis and formation of thrombosis. The axial velocity magnitude percentage difference of up to 2 % and radial velocity difference up to 90 % is found at different sections of the T-junction geometry. The size of flow recirculation zones and their associated separation and reattachment point's locations differ for each model. The wall shear stress also experiences up to 12 % shift in the main tube. A velocity magnitude distribution of the grid cells shows that the Newtonian model is close dynamically to the Casson model while the power law model resembles the Carreau model. ZUSAMMENFASSUNG: Die Rheologie von Blutströmungen ist ein komplexes Phänomen. Gegenwärtig existiert kein allgemein akzeptiertes Modell, um die viskosen Eigenschaften von Blut wiederzugeben. Jedoch gibt es mehrere Varianten unter der allgemeinen Klassifikation von nicht-Newtonschen Modellen, die das Verhalten von Blut mit unterschiedlicher Genauigkeit simulieren. Die Potenzgesetz-, Casson und Carreau-Modelle sind beliebte nicht-New-tonsche Modelle und beeinflussen die hämodynamischen Eigenschaften in vielen Situationen. In dieser Studie wurde die finite Volumenmethode angewandt, um die h

  13. Development of inexpensive blood imaging systems: where are we now?

    Science.gov (United States)

    Chu, Kaiqin; Smith, Zachary J; Wachsmann-Hogiu, Sebastian

    2015-01-01

    Clinical applications in the developing world, such as malaria and anemia diagnosis, demand a change in the medical paradigm of expensive care given in central locations by highly trained professionals. There has been a recent explosion in optical technologies entering the consumer market through the widespread adoption of smartphones and LEDs. This technology commoditization has enabled the development of small, portable optical imaging systems at an unprecedentedly low cost. Here, we review the state-of-the-field of the application of these systems for low-cost blood imaging with an emphasis on cellular imaging systems. In addition to some promising results addressing specific clinical issues, an overview of the technology landscape is provided. We also discuss several key issues that need to be addressed before these technologies can be commercialized.

  14. Evolutionary origins of the blood vascular system and endothelium

    Science.gov (United States)

    Monahan-Earley, Rita; Dvorak, Ann M.; Aird, William C.

    2017-01-01

    Every biological trait requires both a proximate and evolutionary explanation. The field of vascular biology is focused primarily on proximate mechanisms in health and disease. Comparatively little attention has been given to the evolutionary basis of the cardiovascular system. Here, we employ a comparative approach to review the phylogenetic history of the blood vascular system and endothelium. In addition to drawing on the published literature, we provide primary ultrastructural data related to the lobster, earthworm, amphioxus and hagfish. Existing evidence suggests that the blood vascular system first appeared in an ancestor of the triploblasts over 600 million years ago, as a means to overcome the time-distance constraints of diffusion. The endothelium evolved in an ancestral vertebrate some 540–510 million years ago to optimize flow dynamics and barrier function, and/or to localize immune and coagulation functions. Finally, we emphasize that endothelial heterogeneity evolved as a core feature of the endothelium from the outset, reflecting its role in meeting the diverse needs of body tissues. PMID:23809110

  15. Oscillometric continuous blood pressure sensing for wearable health monitoring system

    CERN Document Server

    Gelao, Gennaro; Passaro, Vittorio M N; Perri, Anna Gina

    2015-01-01

    In this paper we present an acquisition chain for the measurement of blood arterial pressure based on the oscillometric method. This method does not suffer from any limitation as the well-known auscultatory method and it is suited for wearable health monitoring systems. The device uses a pressure sensor whose signal is filtered, digitalized and analyzed by a microcontroller. Local analysis allows the evaluation of the systolic and diastolic pressure values which can be used for local alarms, data collection and remote monitoring.

  16. Blood Types

    Science.gov (United States)

    ... blood, safe blood transfusions depend on careful blood typing and cross-matching. The ABO Blood Group System ... that provided by the ABO positive/negative blood typing. For example, sometimes if the donor and recipient ...

  17. Teriparatide (rhPTH) treatment in children with syndromic hypoparathyroidism.

    Science.gov (United States)

    Matarazzo, Patrizia; Tuli, Gerdi; Fiore, Ludovica; Mussa, Alessandro; Feyles, Francesca; Peiretti, Valentina; Lala, Roberto

    2014-01-01

    Subcutaneous recombinant human parathormone [rhPTH (1-34)] has been introduced for hypoparathyroidism treatment, allowing avoidance of vitamin D and calcium side effects. Our objective was to evaluate rhPTH (1-34) safety and efficacy in pediatric patients with genetically proved syndromic hypoparathyroidism. The study was a 2.5-year self-controlled trial on six pediatric patients (four males, two females, age 9.8±5.1 years) with syndromic hypoparathyroidism including three with autoimmune polyendocrinopathy candidiasis ectodermal dysplasia (APECED) syndrome, two with DiGeorge syndrome, and one with hypoparathyroidism-deafness-renal dysplasia syndrome. We compared patients' clinical and biochemical outcome of conventional treatment based on oral administration of calcium (1-1.5 g/day in three doses) plus oral calcitriol (6.5-33 ng/kg per day in two to three doses) with the outcome obtained with rhPTH (1-34) (teriparatide, 12.5 μg bid). Therapy shift was conducted introducing rhPTH (1-34) while progressively withdrawing calcium and vitamin D. Blood calcium, phosphorus, alkaline phosphatase, and urinary calcium-to-creatinine ratio (mg/mg) before and during rhPTH therapy were compared. rhPTH treatment allowed complete calcium and vitamin D withdrawal in two patients, calcium withdrawal in three and reduction of vitamin D dose in two. During rhPTH (1-34), mean blood calcium, phosphorus, and alkaline phosphatase were not significantly modified, whereas significant reduction of the calciuria-to-creatininuria ratio (0.55±0.31 vs. 0.1±0.1, p=0.02) was obtained. The number of tetanic episodes was reduced in four patients during teriparatide treatment compared to conventional treatment. In children with syndromic hypoparathyroidism, substitutive treatment with rhPTH (1-34) maintains adequate blood calcium levels and allows prompt normalization of urinary calcium excretion, through direct action on the kidney and through calcium and vitamin D therapy layoff.

  18. Systematic RH genotyping and variant identification in French donors of African origin.

    Science.gov (United States)

    Kappler-Gratias, Sandrine; Auxerre, Carine; Dubeaux, Isabelle; Beolet, Marylise; Ripaux, Maryline; Le Pennec, Pierre-Yves; Pham, Bach-Nga

    2014-01-01

    RH molecular analysis has enabled the documentation of numerous variants of RHD and RHCE alleles, especially in individuals of African origin. The aim of the present study was to determine the type and frequency of D and/or RhCE variants among blood donors of African origin in France, by performing a systematic RH molecular analysis, in order to evaluate the implications for blood transfusion of patients of African origin. Samples from 316 African blood donors, whose origin was established by their Fy(a-b-) phenotype, were first analysed using the RHD and RHCE BeadChips Kit (BioArray Solutions, Immucor, Warren, NJ, USA). Sequencing was performed when necessary. RHD molecular analysis showed that 26.2% of donors had a variant RHD allele. It allowed the prediction of a partial D in 11% of cases. RHCE molecular analysis showed that 14.2% of donors had a variant RHCE allele or RH [RN or (C)ces] haplotype. A rare Rh phenotype associated with the loss of a high-prevalence antigen or partial RhCE antigens were predicted from RHCE molecular analysis in 1 (0.3%) and 17 (5%) cases, respectively. Systematic RHD and RHCE molecular analysis performed in blood donors of African origin provides transfusion-relevant information for individuals of African origin because of the frequency of variant RH alleles. RH molecular analysis may improve transfusion therapy of patients by allowing better donor and recipient matching, based not only on phenotypically matched red blood cell units, but also on units that are genetically matched with regards to RhCE variants.

  19. A model of blood flow in the mesenteric arterial system

    Directory of Open Access Journals (Sweden)

    Cheng Leo K

    2007-05-01

    Full Text Available Abstract Background There are some early clinical indicators of cardiac ischemia, most notably a change in a person's electrocardiogram. Less well understood, but potentially just as dangerous, is ischemia that develops in the gastrointestinal system. Such ischemia is difficult to diagnose without angiography (an invasive and time-consuming procedure mainly due to the highly unspecific nature of the disease. Understanding how perfusion is affected during ischemic conditions can be a useful clinical tool which can help clinicians during the diagnosis process. As a first step towards this final goal, a computational model of the gastrointestinal system has been developed and used to simulate realistic blood flow during normal conditions. Methods An anatomically and biophysically based model of the major mesenteric arteries has been developed to be used to simulate normal blood flows. The computational mesh used for the simulations has been generated using data from the Visible Human project. The 3D Navier-Stokes equations that govern flow within this mesh have been simplified to an efficient 1D scheme. This scheme, together with a constitutive pressure-radius relationship, has been solved numerically for pressure, vessel radius and velocity for the entire mesenteric arterial network. Results The computational model developed shows close agreement with physiologically realistic geometries other researchers have recorded in vivo. Using this model as a framework, results were analyzed for the four distinct phases of the cardiac cycle – diastole, isovolumic contraction, ejection and isovolumic relaxation. Profiles showing the temporally varying pressure and velocity for a periodic input varying between 10.2 kPa (77 mmHg and 14.6 kPa (110 mmHg at the abdominal aorta are presented. An analytical solution has been developed to model blood flow in tapering vessels and when compared with the numerical solution, showed excellent agreement. Conclusion An

  20. Correlation analysis of hemolytic disease of newborn and RhD negative pregnant women carrying RhD positive fetus%RhD阴性孕妇妊娠RhD阳性胎儿与新生儿溶血病的相关性分析

    Institute of Scientific and Technical Information of China (English)

    何子毅; 刘赴平; 胡应明; 张建强

    2012-01-01

    目的 探讨RhD阴性孕妇产前免疫性抗D抗体的检出率与产后新生儿溶血病(HDN)发病率的相关性.方法 收集2007年1月至2010年12月137例RhD阴性孕妇产前血样及40例产后血样,分析产前抗D抗体的检出率、产后HDN发病率及其与母儿ABO血型配合性的关系.结果 16例(12.60%)RhD阴性孕妇多次妊娠者检测到抗D抗体,10例首次妊娠者均未检出;产前血清抗D抗体的检出率低于产后(P0.05).结论 产前RhD阴性孕妇抗D抗体的检出率与产后发生HDN的相关性尚不明确; ABO血型不配合对RhD阴性孕妇发生RhD-HDN有保护作用,但对产生抗D抗体的检出率无影响.%Objective To explore the correlation of prenatal detection rate of immune anti-D antibody of pregnant women with RhD negative blood type and the incidence rate of postpartum hemolytic disease of newborn(HDN). Methods 137 prenatal blood samples of pregnant women with RhD negative blood type and 40 postpartum blood samples were collected from January 2007 to December 2010. Their prenatal detection rate of anti-D antibody, incidence rate of postpartum HDN and their relationship with maternal-fetal ABO-blood group compatibility were analyzed. Results Anti-D antibody was detected in 16(12. 60%) pregnant women with RhD negative blood type and history of several pregnancies, and was not detected in 10 women in their first pregnancy. The prenatal detection rate of serum anti-D antibody was lower than that after delivery(P0. 05). Conclusion The correlation of prenatal detection rate of anti-D antibody of RhD negative pregnant women and the incidence rate of postpartum HDN is not clear. ABO-blood group incompatibility has a protective effect on RhD negative pregnant women from RhD-HDN, but no effect on detection rate of anti-D antibodies.

  1. Quantum criticality and Lifshitz transition in the Ising system CeRu2Si2: Comparison with YbRh2Si2

    OpenAIRE

    Pourret, A.; Aoki, D.; Boukahil, M.; Brison, J. -P.; Knafo, W.; Knebel, G.; Raymond, S.; Taupin, M.; Onuki, Y.; Flouquet, J.

    2013-01-01

    New thermoelectric power (TEP) measurements on prototype heavy-fermion compounds close to magnetic quantum criticality are presented. The highly sensitive technique of TEP is an unique tool to reveal Fermi surface instabilities, referred here as Lifshitz transitions. The first focus is on the Ising CeRu2Si2 series. Doping CeRu2Si2 with Rh produces a decoupling between the first order metamagnetic transition and the pseudo-metamagnetism observed in the pure compound. Comparison is made with th...

  2. Dielectric and magnetodielectric behavior of polycrystalline Ca{sub 3}CoRhO{sub 6}, a geometrically frustrated magnetic system

    Energy Technology Data Exchange (ETDEWEB)

    Iyer, Kartik K.; Basu, Tathamay, E-mail: tathamay@tifr.res.in; Singh, Kiran; Sampathkumaran, E. V. [Tata Institute of Fundamental Research, HomiBhabha Road, Colaba, Mumbai-400005 (India)

    2015-06-24

    We have investigated dielectric andmagnetodielectric properties of the geometrically frustrated spin -chain compound Ca{sub 3}CoRhO{sub 6}, which is known to undergo ‘partially disordered antiferromagnetic’ transition around 90 K (T{sub 1}) followed by another glassy magnetic transition around 30 K (T{sub 2}). We have observed dielectric glass behavior around 90 K, at the magnetic transition, which indicates that this behavior is predominantly magnetic in its origin. The magnetic field dependent dielectric constant in the magnetically ordered state tracks the features in isothermal magnetization thereby establishingmagnetodielectric coupling in this compound.

  3. Giant crystal field and ferromagnetism in the Kondo system CeRh3B2: Soft-x-ray linear-dichroism study

    Science.gov (United States)

    Yamaguchi, K.; Namatame, H.; Hase, I.; Fujimori, A.; Misu, A.; Kasaya, M.; Kasuya, T.

    1992-10-01

    We have studied the electronic structure of CeRh3B2, which has an anomalously high ferromagnetic ordering temperature, by linear dichroism in the Ce 4d core-level x-ray-absorption spectra. The result indicates an unusually large axial crystal field of order 0.1-0.2 eV along the hexagonal c axis. We propose a possible origin of the giant crystal field and the anomalous ferromagnetism in terms of the strong interatomic Ce 4f-Ce 5d hybridization.

  4. GnRH tandem peptides for inducing an immunogenic response to GnRH-I without cross-reactivity to other GnRH isoforms

    NARCIS (Netherlands)

    Turkstra, J.A.; Schaaper, W.M.M.; Oonk, H.B.; Meloen, R.H.

    2005-01-01

    Gonadotropin releasing hormone (GnRH) occurs in various isoforms in mammals, i.e. GnRH-I (mammalian GnRH), GnRH-II (chicken GnRH-II), GnRH-III (salmon GnRH) and two forms of lamprey GnRH. The function of the latter four molecules have only been partially investigated. Also not much is known about th

  5. Rh isoimmunization in Sub-Saharan Africa indicates need for universal access to anti-RhD immunoglobulin and effective management of D-negative pregnancies

    OpenAIRE

    2010-01-01

    Erhabor Osaro1, Adias Teddy Charles21Department of Blood Sciences, Royal Bolton Hospital NHS Trust UK; 2Department of Medical Laboratory Sciences, Rivers State University of Science and Technology, Port Harcourt, NigeriaAbstract: Transplacental or fetomaternal hemorrhage (FMH) may occur during pregnancy or at delivery and lead to immunization to the D antigen if the mother is Rh-negative and the baby is Rh-positive. This can result in hemolytic disease of the fetus and newborn (HDFN) in subse...

  6. Severe hemolytic disease of the premature newborn due to RH1 incompatibility: a case report

    Science.gov (United States)

    UWINGABIYE, JEAN; ZAHID, HAFID; LABRINI, FAYÇAL; EL KHAZRAJI, ABDELHAK; YAHYAOUI, ANASS; HADEF, RACHID; MESSAOUDI, NEZHA

    2016-01-01

    We report a case of dramatic outcome of severe hemolytic disease in a newborn due to RH1 incompatibility. A newborn with A RH1 blood group was admitted in the Mohammed V Military Teaching Hospital for the problem of hydrops fetalis associated with RH1 incompatibility. The blood group of his mother, aged 31, was AB RH1-negative and that of his 37 year old father was A RH1. The mother had a history of 4 term deliveries, 3 abortions, and 1 living child. There was no prevention by anti-D immunoglobulin postpartum. The mother’s irregular agglutinin test was positive and the pregnancy was poorly monitored. The laboratory tests of the newborn showed a high total serum bilirubin level (30 mg/L) and macrocytic regenerative anemia (Hemoglobin=4 g/dL, mean corpuscular volume = 183 fL, reticulocytes count =176600/m3). The blood smear showed 1256 erythroblasts per 100 leukocytes, Howell–Jolly bodies and many macrocytes. The direct antiglobulin test was positive. He was transfused with red blood cell concentrates and treated with conventional phototherapy. The evolution was unfavourable; he died three days after the death of his mother. The monitoring of these high-risk pregnancies requires specialized centers and a close collaboration between the gynaecologist and the blood transfusion specialist to strengthen the prevention, as well as clinico-biological monitoring in patients with a history of RH1 fetomaternal alloimunization. PMID:27857529

  7. Rh isoimmunization during pregnancy: antenatal prophylaxis.

    Science.gov (United States)

    Bowman, J M; Chown, B; Lewis, M; Pollock, J M

    1978-03-18

    Of 3533 Rh-negative women who began a pregnancy without detectable Rh antibodies, 62 (1.8%) demonstrated evidence of Rh isoimmunization during pregnancy or within 3 days after delivery. All denied transfusions as well as abortions or previous pregnancies not followed by the administration of Rh immune globulin. Rh isoimmunization during pregnancy or within 3 days after delivery, which will not be prevented by the administration of Rh immune globulin after delivery, is the most important cause of residual Rh isoimmunization. A clinical trial of antenatal administration of Rh immune globulin, initially at 34 weeks's and subsequently at 28 and 34 weeks' gestation, in 1357 Rh-negative pregnant women who were delivered of Rh-positive babies, was effective in preventing the development of Rh isoimmunization during pregnancy or within 3 days after delivery. Antenatal prophylaxis with Rh immune globulin will be necessary if the incidence of Rh isoimmunization is to be reduced to its lowest possible level. Antenatal prophylaxis at 28 weeks' gestation is now an insured service in Manitoba.

  8. Oximato bridged Rh$^{\\text{III}}_2$MII and RhIIIMI species (MII = Mn, Co, Ni; MI = Cu, Ag)

    Indian Academy of Sciences (India)

    Indranil Bhattacharyya; Sanjib Ganguly; Bikash Kumar Panda; Animesh Chakravorty

    2008-01-01

    The reaction of [RhCl2(HPhL)(PhL)] with MII(ClO4)2.6H2O in presence of alkali has furnished trinuclear [RhCl2(PhL)2]2M(H2O)2.H2O (HPhL is phenylazobenzaldoxime; M = Mn, Co, Ni). A similar reaction with MI(PPh3)2NO3 yielded binuclear [RhCl2(PhL)2]M(PPh3)2 (M = Cu, Ag). In these molecules the oximato group acts as a bridge between RhIII (bonded at N) and MII or MI (bonded at O). In structurally characterized [RhIIICl2(PhL)2]2Mn(H2O)2.H2O the centrosymmetric distorted octahedral MnO6 coordination sphere is spanned by four oximato oxygen atoms and two water molecules lying in trans position. In the lattice the neighbouring molecules are held together by H2O$\\cdots$H2O$\\cdots$H2O hydrogen bonds generating infinite zigzag chains. The manganese atoms lie parallel to the C-axis, the shortest Mn…Mn distance being 7.992 Å. Magnetic exchange interactions if any are small as seen in room temperature magnetic moments. The manganese system displays a strong EPR signal near = 2.00. In the complex [RhCl2(PhL)2]Cu(PPh3)2 the copper atom is coordinated to two oximato oxygen atoms and the two phosphorus atoms in a distorted tetrahedral geometry. The softness of the phosphine ligand is believed to sustain the stable coordination of hard oximato oxygen to soft CuI. The coordination sphere of the RhIII atom in both the complexes is uniformly trans-RhN4Cl2.

  9. An Analysis of and Recommendations for the Peruvian Blood Collection and Transfusion System

    Science.gov (United States)

    George, Paul E; Vidal, Julio; Garcia, Patricia J

    2016-01-01

    Background Peru experienced a crisis in its blood collection and supply system in the mid-2000s, as contaminated blood led to several transfusion-transmitted infections (TTI), occurring in the backdrop of extremely low voluntary donation rates and a national blood supply shortage. Thus, the Peruvian Ministry of Health (MINSA) implemented a national investigation on the safety and quality of the Peruvian blood collection/transfusion network. Methods Every Peruvian blood bank was evaluated by MINSA from 2007–2008. These evaluations consisted of an update of the national registry of blood banks and visits to each blood bank from MINSA oversight teams. Information was collected on the condition of the blood bank personnel, equipment, supplies, and practices. Further, previously-collected blood at each blood bank was randomly selected and screened for TTI-causing pathogens. Results Uncovered in this investigation was a fragmented, under-equipped, and poorly-staffed blood collection and transfusion network, consisting of 241 independent blood banks and resulting in suboptimal allocation of resources. Further, blood with evidence of TTI-causing pathogens (including Hepatitis B, Hepatitis C, and syphilis) and set for transfusion was discovered at three separate blood banks as part of the random screening process. Conclusion Using the successful reorganizations of national blood supply systems in other Latin American countries as examples, Peru would be well-served to form large, high-volume, regional blood collection and transfusion centers, responsible for blood collection and screening for the entire country. The small, separate blood banks would then be transformed into a network of blood transfusion centers, not responsible for blood collection. This reorganization would allow Peru to better utilize its resources, standardize the blood collection and transfusion process, and increase voluntary donation, resulting in a safer, more abundant national blood product.

  10. Rhesus Negative Woman Transfused With Rhesus Positive Blood: Subsequent Normal Pregnancy Without Anti D production.

    Science.gov (United States)

    Maya, E T; Buntugu, K A; Pobee, F; Srofenyoh, E K

    2015-03-01

    Clinicians sometimes are confronted with the challenge of transfusing haemorrhaging Rhesus (Rh) D negative patients with Rh D positive blood to save their lives. There are concerns about alloimmunization and future haemolytic disease of the newborn in women of the reproductive age. Another fear is transfusion reaction if they receive another Rh D positive blood in future. We present a 32-year-old Rh D negative woman, who had postpartum haemorrhage in her first pregnancy and was transfused with Rh D positive blood because of unavailability of Rh D negative blood. She did not receive anti D immunoglobin but subsequently had a normal term pregnancy of an Rh positive fetus without any detectable anti D antibodies throughout the pregnancy. In life threatening situations from obstetric haemorrhage, transfusion of Rh D negative women with Rh D positive blood should be considered as the last resort.

  11. [Guideline for prevention of RhD alloimmunizationin RhD negative women].

    Science.gov (United States)

    Lubušký, M; Procházka, M; Simetka, O; Holusková, I

    2013-04-01

    Events following which immunoglobulin (Ig) G anti-D should be given to all RhD negative women with no anti-D alloantibodies: First trimester indications (IgG anti-D sufficient dose of 50 μg*) - termination of pregnancy, spontaneous abortion followed by instrumentation, ectopic pregnancy, chorionic villus sampling, partial molar pregnancy; Second and third trimester indications (IgG anti-D sufficient dose of 100 μg*) - amniocentesis, cordocentesis, other invasive prenatal diagnostic or therapeutic procedures, spontaneous or induced abortion, intrauterine fetal death, attempt at external cephalic version of a breech presentation, abdominal trauma, obstetric hemorrhage; Antenatal prophylaxis at 28th weeks of gestation (IgG anti-D sufficient dose of 250 μg*); Delivery of an RhD positive infant** (IgG anti-D sufficient dose of 100 μg*); Minimal dose*: before 20 weeks gestation - 50 μg (250 IU), after 20 weeks gestation*** - 100 μg (500 IU); Timing: as soon as possible, but no later than 72 hours after the event. In cases where prevention of RhD alloimmunization is not performed within 72 hours of a potentially sensitising event, it is still reasonable to administer IgG anti-D within 13 days, and in special cases, administration is still recommended up to a maximum interval of 28 days postpartum; Legend: *administration of a higher dose of IgG anti-D is not a mistake, ** also if the D type is not known, *** simultaneous assessment of the volume of fetomaternal hemorrhage (FMH) to specify the dose is suitable; The FMH volume assessment - If the volume of fetal erythrocytes (red bood cells, RBCs) which entered maternal circulation is assessed, intramuscular administration of IgG anti-D in a dose of 10 μg per 0.5 mL of fetal RBCs or 1 mL of whole fetal blood is indicated. IgG anti-D in a dose of 10 μg administered intramuscularly should cover 0.5 mL of fetal RhD positive RBCs or 1mL of whole fetal blood. FMH is the fetal RBC volume; fetal blood volume is double

  12. A novel system for providing compatible blood to patients during surgery: "self-service" electronic blood banking by nursing staff.

    Science.gov (United States)

    Cheng, G; Chiu, D S; Chung, A S; Wong, H F; Chan, M W; Lui, Y K; Choy, F M; Chan, J C; Chan, A H; Lam, S T; Fan, T C

    1996-04-01

    A good blood bank must be able to provide compatible blood units promptly to operating room patients with minimal wastage. A "self-service" by nursing staff blood banking system that is safe, efficient, and well-accepted has been developed. Specific blood units are no longer assigned to surgical patients who have a negative pretransfusion antibody screen, irrespective of the type of surgery. A computer-generated list of the serial numbers of all group-identical blood units currently in the blood bank inventory is provided for each patient. The units themselves are not labeled with a patient's name. The group O list will be provided for group O patients, the group A list for group A patients, and so forth. Should the patient require transfusion during surgery, the operating room nurses go to the refrigerator, remove any group-identical unit, and check the serial number of the unit against the serial numbers on the patient's list. If the serial number is on that list, the blood bank will accept responsibility for compatibility. The system was implemented in 1995. Since implementation, a total of 2154 patients have undergone operations at this hospital. Thirty-two patients received more than 10 units of red cells each. There were no transfusion errors. The crossmatch-to-transfusion ratio was reduced from 1.67 to 1.12. Turnaround time for supplying additional or urgent units to patients in operating room was shortened from 33 to 2.5 minutes. There was no incidence of a blood unit's serial number not being on the list. Work by nurses and technical staff was reduced by nearly 50 percent. The "self-service" (by nursing staff) blood banking system described is safe and efficient. It saves staff time and can be easily set up.

  13. Homologous radioimmunoassay for salmon gonadotropin releasing hormone s-Gn-RH

    Energy Technology Data Exchange (ETDEWEB)

    Breton, B.; Motin, A. (I.N.R.A., Campus de Beaulieu, 35 - Rennes (France)); Kah, O.; Lemenn, F.; Geoffre, S.; Precigoux, G.; Chambolle, P. (Universite de Bordeaux-I, 33 - Talence (France))

    1984-09-30

    A radioimmunoassay for Salmon Gn-RH (p-gly-His-Trp-Ser-Tyr-Gly-Trp-Leu-Pro-Gly) (NH/sub 2/) has been developed with a sensitivity of 7 pg/assay tube. The system allows the specific detection of an immunological GnRH related substance in the brain and pituitary of three teleost species but not in an elasmobranch the Dogfish. These results are discussed and some Gn-RH contents of the organs are proposed.

  14. Dehybridization of the 4f shell in Si-substituted CeRh3B2

    Science.gov (United States)

    Malik, S. K.; Shenoy, G. K.; Dhar, S. K.; Paulose, P. L.; Vijayaraghavan, R.

    1986-12-01

    The compound CeRh3B2 is known to order magnetically with an unusually high Curie temperature (TC) of 115 K and a low saturation moment of about 0.4μB. We have studied the magnetic behavior of the system CeRh3(B1-xSix)2 (0CeRh3B2 arises because of the Ce 4f hybridization with the neighboring ligands.

  15. [Homologous radioimmunoassay of a hypothalamic factor stimulating salmon pituitary gonodatropic function (sGnRH)].

    Science.gov (United States)

    Breton, B; Motin, A; Kah, O; Lemenn, F; Geoffre, S; Precigoux, G; Chambolle, P

    1984-01-01

    A radioimmunoassay for Salmon Gn-RH p-gly-His-Trp-Ser-Tyr-Gly-Trp- Leu-Pro-Gly) (NH2) has been developed with a sensitivity of 7 pg/assay tube. The system allows the specific detection of an immunological GnRH related substance in the brain and pituitary of three teleost species but not in an elasmobranch the Dogfish. these results are discussed and some Gn-RH contents of the organs are proposed.

  16. In-vitro laser anemometry blood flow systems

    Science.gov (United States)

    Liepsch, Dieter W.; Poll, Axel; Pflugbeil, Gottlieb

    1993-08-01

    Lasers are used in a wide variety of medical applications. While laser catheters have been developed for highly accurate velocity measurements these are invasive; noninvasive techniques are more desirable but not as precise. The laser is, however, a great tool for in vitro measurements. Several groups internationally are using the laser in the study of local velocity distribution in microscopic areas of specially constructed models. Laser Doppler anemometry is widely used to measure the local, time-dependent velocities, while phase Doppler anemometry has been developed to measure particle size, distribution and velocity. Most recently, laser analyzer techniques have been developed for analyzing the particle size of two phase flow systems. It has become increasingly important for physicians to visualize blood flow. In addition to the techniques mentioned above, several laser sheet techniques have been developed for precise measurements. This paper presents a short review of laser techniques and shows some applications especially for the laser-Doppler anemometer.

  17. Blood pressure morning surge, exercise blood pressure response and autonomic nervous system.

    Science.gov (United States)

    Tanindi, Asli; Ugurlu, Murat; Tore, Hasan Fehmi

    2015-08-01

    We investigated blood pressure (BP) response to exercise with respect to BP morning surge (MS), and the association between MS, exercise treadmill test (ETT) and heart rate variability (HRV) indices. Eighty-four healthy subjects without hypertension were enrolled. Ambulatory BP monitoring and 24-hour Holter recordings were obtained for sleep-trough MS and HRV indices: low-frequency (LF) component, high-frequency (HF) component and LF/HF ratio. ETT was performed, and BPs were obtained at rest, end of each stage, and recovery. Third-minute heart rate recovery (HRR) and BP recovery ratio (BPRR) were calculated. When analysed in quartiles of MS, systolic BP at low workloads was higher in the highest than in the lowest quartile, although maximum BPs at maximum exercise were not significantly different. BPRR was highest in the highest quartile in contrast to HRR, which was lowest in the highest quartile. LF/HF was highest during both at daytime and night-time in the highest quartile. BPRR and LF/HF were positively, and HRR was inversely associated with MS. Subjects with a high MS have higher BP at low workloads, at which most daily activities are performed, and impairment in some indices, which indirectly reflect the autonomic nervous system.

  18. Immunomodulating effect of blood transfusion: is storage time important?

    DEFF Research Database (Denmark)

    Mynster, T; Dybkjoer, E; Kronborg, Gitte;

    1998-01-01

    in stimulating TNF-alpha and IL-2 release in an ex vivo assay. METHODS: Supernatants of 10 units of whole blood and 10 units of SAGM blood were collected after 1, 21 and 35 days of standard blood bank storage. Heparinized blood from 20 healthy volunteers (as 'recipients'), corresponding in ABO and Rh type......OBJECTIVES: TNF-alpha and IL-2 are important cytokines in macrophage and T-lymphocyte activity against infection and dissemination of malignant cells. We studied the influence of supernatants from stored whole blood and buffy-coat-depleted SAGM (saline, adenine, glucose and mannitol) blood...... to the stored blood, were used in a culture system with LPS and PHA as stimulators of TNF-alpha and IL-2 release. The effect of added supernatants, from either stored whole blood or SAGM blood, on cytokine release was evaluated compared to saline as control. TNF-alpha concentration was analyzed by ELISA after...

  19. Nanoscale drug delivery systems and the blood-brain barrier.

    Science.gov (United States)

    Alyautdin, Renad; Khalin, Igor; Nafeeza, Mohd Ismail; Haron, Muhammad Huzaimi; Kuznetsov, Dmitry

    2014-01-01

    The protective properties of the blood-brain barrier (BBB) are conferred by the intricate architecture of its endothelium coupled with multiple specific transport systems expressed on the surface of endothelial cells (ECs) in the brain's vasculature. When the stringent control of the BBB is disrupted, such as following EC damage, substances that are safe for peripheral tissues but toxic to neurons have easier access to the central nervous system (CNS). As a consequence, CNS disorders, including degenerative diseases, can occur independently of an individual's age. Although the BBB is crucial in regulating the biochemical environment that is essential for maintaining neuronal integrity, it limits drug delivery to the CNS. This makes it difficult to deliver beneficial drugs across the BBB while preventing the passage of potential neurotoxins. Available options include transport of drugs across the ECs through traversing occludins and claudins in the tight junctions or by attaching drugs to one of the existing transport systems. Either way, access must specifically allow only the passage of a particular drug. In general, the BBB allows small molecules to enter the CNS; however, most drugs with the potential to treat neurological disorders other than infections have large structures. Several mechanisms, such as modifications of the built-in pumping-out system of drugs and utilization of nanocarriers and liposomes, are among the drug-delivery systems that have been tested; however, each has its limitations and constraints. This review comprehensively discusses the functional morphology of the BBB and the challenges that must be overcome by drug-delivery systems and elaborates on the potential targets, mechanisms, and formulations to improve drug delivery to the CNS.

  20. Rh(0)/Rh(iii) core-shell nanoparticles as heterogeneous catalysts for cyclic carbonate synthesis.

    Science.gov (United States)

    Jung, Younjae; Shin, Taeil; Kim, Kiseong; Byun, Hyeeun; Cho, Sung June; Kim, Hyunwoo; Song, Hyunjoon

    2016-12-22

    Rh(0)/Rh(iii) core-shell nanoparticles were prepared by surface oxidation of Rh nanoparticles with N-bromosuccinimide. They were employed as heterogeneous catalysts for cyclic carbonate synthesis from propylene oxide and CO2, and exhibited high activity and excellent recyclability due to Lewis acidic Rh(iii) species on the shells.

  1. Synthesis, crystal structure and electronic structure of the binary phase Rh2Cd5

    Science.gov (United States)

    Koley, Biplab; Chatterjee, S.; Jana, Partha P.

    2017-02-01

    A new phase in the Rh-Cd binary system - Rh2Cd5 has been identified and characterized by single crystal X-ray diffraction and Energy dispersive X-ray analysis. The stoichiometric compound Rh2Cd5 crystallizes with a unit cell containing 14 atoms, in the orthorhombic space group Pbam (55). The crystal structure of Rh2Cd5 can be described as a defect form of the In3Pd5 structure with ordered vacancies, formed of two 2D atomic layers with the stacking sequence: ABAB. The A type layers consist of (3.6.3.6)-Kagomé nets of Cd atoms while the B type layers consist of (35) (37)- nets of both Cd and Rh atoms. The stability of this line phase is investigated by first principle electronic structure calculations on the model of ordered Rh2Cd5.

  2. Magnetic properties of the Ce-Rh binary phases

    Energy Technology Data Exchange (ETDEWEB)

    Kappler, J.P.; Lehmann, P.; Schmerber, G. (Strasbourg-1 Univ., 67 (FR). Groupe d' Etude des Materiaux Metalliques); Nieva, G.; Sereni, J.G. (Comision Nacional de Energia Atomica, San Carlos de Bariloche (AR). Centro Atomico Bariloche)

    1988-12-01

    Crystallographic, magnetic and resistivity studies on the Ce-Rh binary phases clearly define two Ce ground state regions: (i) CeRh{sub 3}, CeRh{sub 2} and CeRh as intermediate valence compounds and (ii) Ce{sub 5}Rh{sub 4}, Ce{sub 3}Rh{sub 2} Ce{sub 5}Rh{sub 3} and Ce{sub 7}Rh{sub 3} with magnetic transitions at low temperature.

  3. Code development of the national hemovigilance system and expansion strategies for hospital blood banks

    Directory of Open Access Journals (Sweden)

    Kim Jeongeun

    2012-01-01

    Full Text Available Objectives : The aims of this study were to develop reportable event codes that are applicable to the national hemovigilance systems for hospital blood banks, and to present expansion strategies for the blood banks. Materials and Methods : The data were obtained from a literature review and expert consultation, followed by adding to and revising the established hemovigilance code system and guidelines to develop reportable event codes for hospital blood banks. The Medical Error Reporting System-Transfusion Medicine developed in the US and other codes of reportable events were added to the Korean version of the Biologic Products Deviation Report (BPDR developed by the Korean Red Cross Blood Safety Administration, then using these codes, mapping work was conducted. We deduced outcomes suitable for practice, referred to the results of the advisory councils, and conducted a survey with experts and blood banks practitioners. Results : We developed reportable event codes that were applicable to hospital blood banks and could cover blood safety - from blood product safety to blood transfusion safety - and also presented expansion strategies for hospital blood banks. Conclusion : It was necessary to add 10 major categories to the blood transfusion safety stage and 97 reportable event codes to the blood safety stage. Contextualized solutions were presented on 9 categories of expansion strategies of hemovigilance system for the hospital blood banks.

  4. Microcontroller-based system for collecting anaerobic blood samples from a running greyhound.

    Science.gov (United States)

    Schmalzried, R T; Toll, P W; Devore, J J; Fedde, M R

    1992-04-01

    Many physiological variables change rapidly in the blood during sprint exercise. To characterize the dynamics and extent of these changes, blood samples must be obtained during exercise. We describe herein a portable, microcontroller-based system used to automatically obtain repeated, anaerobic, arterial blood samples from greyhounds before, during, and following a race on a track. In addition, the system also records the blood temperature in the pulmonary artery each time a blood sample is taken. The system has been tested for more than 2 years and has proven to be reliable and effective.

  5. Rh antigens and phenotype frequencies of the Ibibio, Efik, and Ibo ethnic nationalities in Calabar, Nigeria.

    Science.gov (United States)

    Jeremiah, Z A; Odumody, C

    2005-01-01

    This report forms part of the study on the Rh phenotypes within the various ethnic nationalities in the south-south region of Nigeria. The aim is to demonstrate the Rh polymorphisms among the people of African descent. The frequencies of Rh blood group antigens and phenotypes of the Ibibio, Efik, and Ibo ethnic nationalities in Calabar municipality, Nigeria, were determined using standard serologic techniques. Of the 720 Calabar individuals tested, the frequencies of the Rh antigens within the nationalities were c (100%), e (96.38%), D (96.38%), E (15.22%), and C (3.62%) for the Ibibios; c (100%), e (95.60%), D (96.70%), E (21.98%), and C (0%) for the Efiks; and c (100%), e (94.29%), D (91.43%), E (28.57%), and C (2.86%) for the Ibos. The overall frequencies of the Rh antigens in these 720 individuals were c (100%), e (95.56%), D (94.44%), E (18.89%), and C (2.78%). Forty (5.56%) were found to be D-, while all were found to possess the c antigen. The most frequently occurring Rh phenotype was Dccee, with a frequency of 73.61 percent. The alternative allele, C, did not appear in homozygous form (CC) in the population tested. This study further demonstrates the variability of Rh blood group phenotypes in Nigeria and Africa.

  6. Rh sensitized pregnancy with high ICT titre with favourable foetal outcome: a rare case

    Directory of Open Access Journals (Sweden)

    Namrata Saxena

    2015-10-01

    Full Text Available Leiomyosarcoma Rhesus (Rh isoimmunization is the development of maternal IgG antibodies against fetal Rh Red Blood Cell (RBC antigens. Transplacental passage of maternal Rh antibody (IgG anti-D may result in Hemolytic Disease of the Newborn (HDN, a condition characterized by hemolysis, severe fetal anemia and generalized fetal edema (hydrops fetalis. The severity of Rh hemolytic disease may be assessed in the Immunized patient by several different means which include: past obstetric history, maternal antibody titer, ultrasonography, amniotic fluid bilirubin quantification, and percutaneous umbilical blood sampling. A past history of fetal demise, hydrops fetalis, intrauterine transfusion or neonatal exchange transfusion implies that the present pregnancy is or will be severely affected because Rh disease effects on the fetus generally recur or present progressively earlier or more severely with each successive Rh positive pregnancy. We present here a rare case of Rh sensitized pregnancy with high ICT titre who had normal fetal outcome. [Int J Reprod Contracept Obstet Gynecol 2015; 4(5.000: 1574-1576

  7. Structure and catalytic reactivity of Rh oxides

    DEFF Research Database (Denmark)

    Gustafson, J.; Westerström, R.; Resta, A.;

    2009-01-01

    Using a combination of experimental and theoretical techniques, we show that a thin RhO2 surface oxide film forms prior to the bulk Rh2O3 corundum oxide on all close-packed single crystal Rh surfaces. Based on previous reports, we argue that the RhO2 surface oxide also forms on vicinal Rh surfaces...... as well as on Rh nanoparticles. The detailed structure of this film was previously determined using UHV based techniques and density functional theory. In the present paper, we also examine the structure of the bulk Rh2O3 corundum oxide using surface X-ray diffraction. Being armed with this structural...... surface oxide film. In the case of Pt25Rh75(1 0 0), our measurements demonstrate that the formation of bulk Rh2O3 corundum oxide poisons the reaction, and argue that this is also valid for all other Rh surfaces. Our study implies that the CO oxidation reaction over Rh surfaces at realistic conditions...

  8. SIBAS: a blood bank information system and its 5-year implementation at Macau.

    Science.gov (United States)

    Li, Bing Nan; Chao, Sam; Chui Dong, Ming

    2007-05-01

    Automation systems and information technology can greatly help medical facilities to improve their working efficiency and optimize the whole workflow. This article surveys electronic information management in blood donation and transfusion service, and explores the rationale and archetype of blood bank information systems, then exemplifies a successful in-running system-Sistema Integrado de Bancos de Sangue (SIBAS), which is developed by the Institute of Systems and Computer Engineering of Macau (INESC-Macau) in cooperation with the Macau Blood Transfusion Center (CTS-Macau). Its implementation and the related lessons are briefly introduced too. In essence, this article is oriented to serve as a reference of contemporary blood bank information systems.

  9. Comparison of thermomagnetic history effects in weakly pinned single crystals of 3Rh4Sn13(R=Yb, Ca)

    Indian Academy of Sciences (India)

    S Sarkar; S Ramakrishnan; A K Grover; C V Tomy; G Balakrishnan; D McK Paul

    2002-05-01

    A comparative study of the thermomagnetic memory effects of c in two weakly pinned low c superconductors, Ca3Rh4Sn13 (CaRhSn) and Yb3Rh4Sn13 (YbRhSn), is presented. In both the systems, the peak effect (PE) phenomenon appears as an order–disorder transformation through stepwise amorphization of the flux line lattice (FLL). However, in CaRhSn, we can witness another disorder-driven transition (Bragg glass (BG) to a vortex glass (VG)) in a distinct manner as in a single crystal of high c YBa2Cu3O7- for $H||c$.

  10. Fluorocarbene, fluoroolefin, and fluorocarbyne complexes of Rh.

    Science.gov (United States)

    Pell, Christopher J; Zhu, Yanjun; Huacuja, Rafael; Herbert, David E; Hughes, Russell P; Ozerov, Oleg V

    2017-04-01

    The manuscript reports the synthesis, characterization, and analysis of electronic structure in a series of complexes of small perfluorocarbon ligands with the (PNP)Rh fragment (where PNP is a diarylamido/bis(phosphine) pincer ligand). Reactions of (PNP)Rh(TBE) as the source of (PNP)Rh with CHF3 and C2HF5 produced perfluoroalkylidene complexes (PNP)Rh 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 1111111111111111111111111111111111 1111111111111111111111111111111111 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 1111111111111111111111111111111111 1111111111111111111111111111111111 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 0000000000000000000000000000000000 CF2 and (PNP)RhC(F)(CF3). (PNP)RhCF2 could also be obtained via the reaction of (PNP)Rh(TBE) with Me3SiCF3/CsF, with an admixture of (PNP)Rh(C2F4), where TBE = tert-butylethylene. Abstraction of fluoride from these neutral (PNP)RhC x F y complexes was successful, although only abstraction from (PNP)RhCF2 allowed unambiguous identification of the Rh product, [(PNP)Rh 0000000000000000000000000000000000 0000000000000000000000000000000000

  11. A₁A₂BO and Rh gene frequencies among six populations of Jammu and Kashmir, India.

    Science.gov (United States)

    Fareed, Mohd; Hussain, Ruqaiya; Shah, Ahsana; Afzal, Mohammad

    2014-04-01

    A study was undertaken to record gene frequencies of ABO blood groups, their subtypes and Rh antigen for six different endogamous groups including a tribal population. The ABO phenotypic frequency varies among six different populations showing significant difference (pI(B)>I(A1)>I(A2), except Syeds (I(O)>I(A1)>I(B)>I(A2)). The rhesus protein (Rh) phenotypic frequency (pKashmir.

  12. Prevention of Rh isoimmunization after spontaneous, massive fetomaternal hemorrhage.

    Science.gov (United States)

    Graham, E M; Freedman, L J; Forouzan, I; Morgan, M A

    1996-09-01

    Massive fetomaternal hemorrhage has been associated with numerous risk factors and can occur spontaneously. The risk is probably greater when a woman who is Rh negative has an ABO-compatible fetus. We report the case of a gravid, A-negative unsensitized patient who came to the hospital at term complaining of decreased fetal movement over the previous 24 hours. During evaluation, the fetal heart rate was found to have decreased beat-to-beat variability and repetitive late decelerations, and the mother was delivered of a 3,005 gA-positive neonate (by cesarean section) with a hemoglobin level of 2.9 g/dL. An acid elution test showed 400 mL of fetal blood in the maternal circulation, and the patient received 23 ampules (6,900 micrograms) of Rh immune globulin postpartum. The patient's condition was observed for 156 days after delivery; she did not become sensitized to the Rh factor. A massive fetomaternal hemorrhage can occur without any antecedent risk factors, with a risk of subsequent morbidity to the neonate. Sensitization can be prevented by prompt administration of adequate amounts of Rh immune globulin.

  13. [Development of an automatic pneumatic tourniquet system that determines pressures in synchrony with systolic blood pressure].

    Science.gov (United States)

    Liu, Hongyun; Li, Kaiyuan; Zhang, Zhengbo; Guo, Junyan; Wang, Weidong

    2012-11-01

    The correlation coefficients between arterial occlusion pressure and systolic blood pressure, diastolic blood pressure, limb circumference, body mass etc were obtained through healthy volunteer experiments, in which tourniquet were applied on upper/lower extremities. The prediction equations were derived from the data of experiments by multiple regression analysis. Based on the microprocessor C8051F340, a new pneumatic tourniquet system that can determine tourniquet pressure in synchrony with systolic blood pressure was developed and verified the function and stability of designed system. Results showed that the pneumatic tourniquet which automatically adjusts occlusion pressure in accordance with systolic blood pressure could stop the flow of blood to get a bloodless field.

  14. Mobile Personal Health System for Ambulatory Blood Pressure Monitoring

    Directory of Open Access Journals (Sweden)

    Luis J. Mena

    2013-01-01

    Full Text Available The ARVmobile v1.0 is a multiplatform mobile personal health monitor (PHM application for ambulatory blood pressure (ABP monitoring that has the potential to aid in the acquisition and analysis of detailed profile of ABP and heart rate (HR, improve the early detection and intervention of hypertension, and detect potential abnormal BP and HR levels for timely medical feedback. The PHM system consisted of ABP sensor to detect BP and HR signals and smartphone as receiver to collect the transmitted digital data and process them to provide immediate personalized information to the user. Android and Blackberry platforms were developed to detect and alert of potential abnormal values, offer friendly graphical user interface for elderly people, and provide feedback to professional healthcare providers via e-mail. ABP data were obtained from twenty-one healthy individuals (>51 years to test the utility of the PHM application. The ARVmobile v1.0 was able to reliably receive and process the ABP readings from the volunteers. The preliminary results demonstrate that the ARVmobile 1.0 application could be used to perform a detailed profile of ABP and HR in an ordinary daily life environment, bedsides of estimating potential diagnostic thresholds of abnormal BP variability measured as average real variability.

  15. Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons

    Science.gov (United States)

    Cortés-Campos, Christian; Letelier, Joaquín; Ceriani, Ricardo; Whitlock, Kathleen E.

    2015-01-01

    ABSTRACT Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide essential for fertility in vertebrates. Human male patients lacking GnRH and treated with hormone therapy can remain fertile after cessation of treatment suggesting that new GnRH neurons can be generated during adult life. We used zebrafish to investigate the neurogenic potential of the adult hypothalamus. Previously we have characterized the development of GnRH cells in the zebrafish linking genetic pathways to the differentiation of neuromodulatory and endocrine GnRH cells in specific regions of the brain. Here, we developed a new method to obtain neural progenitors from the adult hypothalamus in vitro. Using this system, we show that neurospheres derived from the adult hypothalamus can be maintained in culture and subsequently differentiate glia and neurons. Importantly, the adult derived progenitors differentiate into neurons containing GnRH and the number of cells is increased through exposure to either testosterone or GnRH, hormones used in therapeutic treatment in humans. Finally, we show in vivo that a neurogenic niche in the hypothalamus contains GnRH positive neurons. Thus, we demonstrated for the first time that neurospheres can be derived from the hypothalamus of the adult zebrafish and that these neural progenitors are capable of producing GnRH containing neurons. PMID:26209533

  16. Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH neurons

    Directory of Open Access Journals (Sweden)

    Christian Cortés-Campos

    2015-09-01

    Full Text Available Gonadotropin-releasing hormone (GnRH is a hypothalamic decapeptide essential for fertility in vertebrates. Human male patients lacking GnRH and treated with hormone therapy can remain fertile after cessation of treatment suggesting that new GnRH neurons can be generated during adult life. We used zebrafish to investigate the neurogenic potential of the adult hypothalamus. Previously we have characterized the development of GnRH cells in the zebrafish linking genetic pathways to the differentiation of neuromodulatory and endocrine GnRH cells in specific regions of the brain. Here, we developed a new method to obtain neural progenitors from the adult hypothalamus in vitro. Using this system, we show that neurospheres derived from the adult hypothalamus can be maintained in culture and subsequently differentiate glia and neurons. Importantly, the adult derived progenitors differentiate into neurons containing GnRH and the number of cells is increased through exposure to either testosterone or GnRH, hormones used in therapeutic treatment in humans. Finally, we show in vivo that a neurogenic niche in the hypothalamus contains GnRH positive neurons. Thus, we demonstrated for the first time that neurospheres can be derived from the hypothalamus of the adult zebrafish and that these neural progenitors are capable of producing GnRH containing neurons.

  17. Performance evaluation of continuous blood sampling system for PET study. Comparison of three detector-systems

    CERN Document Server

    Matsumoto, K; Sakamoto, S; Senda, M; Yamamoto, S; Tarutani, K; Minato, K

    2002-01-01

    To measure cerebral blood flow with sup 1 sup 5 O PET, it is necessary to measure the time course of arterial blood radioactivity. We examined the performance of three different types of continuous blood sampling system. Three kinds of continuous blood sampling system were used: a plastic scintillator-based beta detector (conventional beta detector (BETA)), a bismuth germinate (BGO)-based coincidence gamma detector (Pico-count flow-through detector (COINC)) and a Phoswich detector (PD) composed by a combination of plastic scintillator and BGO scintillator. Performance of these systems was evaluated for absolute sensitivity, count rate characteristic, sensitivity to background gamnra photons, and reproducibility for nylon tube geometry. The absolute sensitivity of the PD was 0.21 cps/Bq for sup 6 sup 8 Ga positrons at the center of the detector. This was approximately three times higher than BETA, two times higher than COINC. The value measured with BETA was stable, even when background radioactivity was incre...

  18. Significance of serological monitoring in a Bombay Rh (D) negative phenotype pregnant woman: a case report.

    Science.gov (United States)

    Jain, Ashish; Kumawat, Vijay; Patil, Sandeep S; Kumar, Praveen; Marwaha, Neelam; Sharma, Ratti Ram

    2012-12-01

    A 32 year old Indian female was referred to our hospital at 32 weeks of gestation because of difficulty in blood group determination and further antenatal care. The results of cell and serum grouping of her blood sample were suggestive of Bombay (O(h)) Rh (D) negative phenotype. An indirect antiglobulin test (IAT) using a pool of red cells from two Bombay Rh (D) positive blood donors gave negative result using the tube as well as the gel technique (LISS-Coombs Card, BioRad, Switzerland), thus ruling out anti-D antibody in her serum. The anti-H titer was 16 (tube technique) and with dithiothreitol (DTT) treated patient's serum the antibody screening was negative suggestive of IgM type of anti-H antibodies. Within the patient's family, only one member (younger sister) was of O(h) phenotype and also was Rh (D) negative. The baby was born vaginally at 38+6 weeks of gestation and was non-hydropic with a packed cell volume (PCV) of 55%. The baby's blood group was AB Rh (D) negative and the cord blood direct antiglobulin test (DAT) was negative. Thus, a careful serological testing of O(h) phenotype antenatal women especially with Rh (D) negative phenotype is of utmost importance in determining the isoimmunization status. Copyright © 2012 Elsevier Ltd. All rights reserved.

  19. Effect of volume expansion on systemic hemodynamics and central and arterial blood volume in cirrhosis

    DEFF Research Database (Denmark)

    Møller, Søren; Bendtsen, Flemming; Henriksen, Jens Henrik

    1995-01-01

    and in controls. METHODS: Thirty-nine patients with cirrhosis (12 patients with Child-Turcotte class A, 14 with class B, and 13 with class C) and 6 controls were studied. During hepatic vein catheterization, cardiac output, systemic vascular resistance, central and arterial blood volume, noncentral blood volume...... in patients with either class B or class C. Conversely, the noncentral blood volume increased in patients with class B and C. In both patients and controls, the cardiac output increased and the systemic vascular resistance decreased, whereas the mean arterial blood pressure did not change significantly......BACKGROUND & AIMS: Systemic vasodilatation in cirrhosis may lead to hemodynamic alterations with reduced effective blood volume and decreased arterial blood pressure. This study investigates the response of acute volume expansion on hemodynamics and regional blood volumes in patients with cirrhosis...

  20. BIOEQUIVALENCE BETWEEN rhGH FOR RECONSTITUTION AND READY-TO-USE rhGH IN TWO LIQUID FORMULATIONS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objective To evaluate the bioequivalence between recombinant human growth hormone ( rhGH) for reconstitution, and two dosages of liquid formulation of rhGH [ (15IU)5mg or ( 30IU) l0mg per 3ml].Methods The study drugs were tested in a randomized, single-blind and three-period crossover studies in 24 healthy male subjects. The three drugs were administered by subcutaneous injection at a dose of 0. 2IU/kg body weight. A continuous somatostatin infusion was given in order to suppress the secretion of endogenous GH. The venous blood samples were drawn at different time points to test the serum concentration of GH. The pharmacokinetic parameters were analyzed by statistical methods. Results 90% confidence intervals (CI) of AUC0-24h among three products were all within 80% -125% interval (103.4% -116. 5%, 105. 7% -119. 6% and 91.9% -103. 7%,respectively), and the CIs of Cmax among three products were all within 70%-143% interval ( 91.9%-114. 0%,103. 7%-127. 2% and 81.6%-97. 4%, respectively). There was no statisitical difference of tmax among all the three products. Conclusion These data demonstrate that there is bioequivalence between rhGH for reconstitution and two liquid formulations of rhGH.

  1. BLOOD DONORS CAMPAIGN

    CERN Document Server

    2000-01-01

    A blood donors campaign, organized by the Établissement de Transfusion de Rhône-Alpes will be held at CERN on Tuesday 14 November 2000 in restaurant nr 2, from 8.30 to 16.30 hrs If you already have a card giving your blood group, please bring this with you.

  2. Chemoselective hydrogenation of arenes by PVP supported Rh nanoparticles.

    Science.gov (United States)

    Ibrahim, Mahmoud; Poreddy, Raju; Philippot, Karine; Riisager, Anders; Garcia-Suarez, Eduardo J

    2016-12-06

    Polyvinylpyrrolidone-stabilized Rh nanoparticles (RhNPs/PVP) of ca. 2.2 nm in size were prepared by the hydrogenation of the organometallic complex [Rh(η(3)-C3H5)3] in the presence of PVP and evaluated as a catalyst in the hydrogenation of a series of arene substrates as well as levulinic acid and methyl levulinate. The catalyst showed excellent activity and selectivity towards aromatic ring hydrogenation compared to other reported transition metal-based catalysts under mild reaction conditions (room temperature and 1 bar H2). Furthermore, it was shown to be a highly promising catalyst for the hydrogenation of levulinic acid and methyl levulinate in water leading to quantitative formation of the fuel additive γ-valerolactone under moderate reaction conditions compared to previously reported catalytic systems.

  3. Crystallographic shear mechanisms in Rh one-dimensional oxides

    Science.gov (United States)

    Hernando, María; Boulahya, Khalid; Parras, Marina; González-Calbet, José M.

    2005-02-01

    Electron diffraction and high resolution electron microscopy have been used to characterize two new one-dimensional superstructures in the A sbnd Rh sbnd O system (A = Ca, Sr) related to the 2H-ABO 3-type. They are formed by the intergrowth of n A 3A'BO 6 blocks, showing the Sr 4RhO 6-type, with A 12A' 2B 8O 30 blocks, constituted by two A 3O 9 and two A 3A'O 6 layers alternating in the stacking sequence 1:1, leading to the A 27A' 7B 13O 60 ( n=5) and A 30A' 8B 14O 66 ( n=6) compositions. A crystallographic shear mechanism is proposed to describe the structural relationship between Sr 4RhO 6 (A 3A'BO 6-type) and the new superstructures.

  4. Finite-temperature magnetism of FeRh compounds

    Science.gov (United States)

    Polesya, S.; Mankovsky, S.; Ködderitzsch, D.; Minár, J.; Ebert, H.

    2016-01-01

    The temperature dependent stability of the magnetic phases of FeRh were investigated by means of total energy calculations with magnetic disorder treated within the uncompensated disordered local moment approach. In addition, Monte Carlo simulations based on the extended Heisenberg model have been performed, using exchange coupling parameters obtained from first principles. The crucial role and interplay of two factors in the metamagnetic transition in FeRh has been revealed, namely the dependence of the Fe-Fe exchange coupling parameters on the temperature-governed degree of magnetic disorder in the system and the stabilizing nature of the induced magnetic moment on Rh-sites. An important observation is the temperature dependence of these two competing factors.

  5. Magnetic moment distribution of ferromagnetic Ni--Rh alloys. [3 to 35 at. % Rh

    Energy Technology Data Exchange (ETDEWEB)

    Cable, J.W.; Wollan, E.O.

    1976-08-01

    The diffuse scattering of polarized and unpolarized neutrons was used to determine the spatial distribution of the magnetic moment for ferromagnetic Ni-Rh alloys. The average Ni moment remains near 0.6 ..mu../sub B/ to 12 at. percent Rh and then decreases toward zero at the critical concentration of 37 at. percent Rh. There is an initial rapid decrease in the Rh moment that follows a P/sub 12/ dependence and corresponds to a moment of 2..mu../sub B/ for isolated Rh atoms. The data indicate moment fluctuations at both the Ni and the Rh sites that are associated with local environment.

  6. GnRH neuron firing and response to GABA in vitro depend on acute brain slice thickness and orientation.

    Science.gov (United States)

    Constantin, Stephanie; Piet, Richard; Iremonger, Karl; Hwa Yeo, Shel; Clarkson, Jenny; Porteous, Robert; Herbison, Allan E

    2012-08-01

    The GnRH neurons exhibit long dendrites and project to the median eminence. The aim of the present study was to generate an acute brain slice preparation that enabled recordings to be undertaken from GnRH neurons maintaining the full extent of their dendrites or axons. A thick, horizontal brain slice was developed, in which it was possible to record from the horizontally oriented GnRH neurons located in the anterior hypothalamic area (AHA). In vivo studies showed that the majority of AHA GnRH neurons projected outside the blood-brain barrier and expressed c-Fos at the time of the GnRH surge. On-cell recordings compared AHA GnRH neurons in the horizontal slice (AHAh) with AHA and preoptic area (POA) GnRH neurons in coronal slices [POA coronal (POAc) and AHA coronal (AHAc), respectively]. AHAh GnRH neurons exhibited tighter burst firing compared with other slice orientations. Although α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) excited GnRH neurons in all preparations, γ-aminobutyric acid (GABA) was excitatory in AHAc and POAc but inhibitory in AHAh slices. GABA(A) receptor postsynaptic currents were the same in AHAh and AHAc slices. Intriguingly, direct activation of GABA(A) or GABA(B) receptors respectively stimulated and inhibited GnRH neurons regardless of slice orientation. Subsequent experiments indicated that net GABA effects were determined by differences in the ratio of GABA(A) and GABA(B) receptor-mediated effects in "long" and "short" dendrites of GnRH neurons in the different slice orientations. These studies document a new brain slice preparation for recording from GnRH neurons with their extensive dendrites/axons and highlight the importance of GnRH neuron orientation relative to the angle of brain slicing in studying these neurons in vitro.

  7. Hemotype全自动血型分析系统在血型鉴定中的应用研究%Research on hemotype fully automatic blood grouping instrument for identification of blood groups

    Institute of Scientific and Technical Information of China (English)

    马晓军; 李海军; 李凌波

    2012-01-01

    目的 探讨全自动血型分析系统在ABO、RhD血型鉴定中的应用.方法 使用微板法自动血型分析系统,在一次性U形板上通过加样、离心、振荡、判读完成血型检测并发送报告.结果 8013份献血者ABO血型一次性准确定型率99.91%(8006/8013),7例正反定型不一致(含O细胞凝集),Rh(D)阴性4例.60例乳糜标本ABO血型正反定型一致.结论 全自动血型分析系统进行ABO正反定型及RhD血型检测更安全可靠,实验操作实现了标准化、自动化,检测结果可永久保存、便于查询.%Objective To evaluate fully automatic blood grouping instrument for identification of blood groups of ABO and RhD. Methods Using automatic detection system distribute red cell or plasma in microplate. After reacting with blood grouping reagent, observe the results and report on LAN (Local Area Network). Results The rates of first proper ABO grouping were 99. 91% (8006/8013).7 out of 8013 O-group donor blood samples showed agglutination in the reverse ABO typing. 1 sample was found to RhD negative. The rates of first proper ABO grouping were 100% ( 60/60) of chylemia. Conclusion Fully automatic blood grouping instrument for detecting blood groups of ABO and RhD is safer,Standardization and automatic of blood grouping experiments has been achieved. The results can be stored timelessly, and are convenient for query.

  8. Influence of rhTNFR:FC on expression of cartilage oligomeric matrix protein in synovial fluid and peripheral blood among juvenile idiopathic arthritis%注射用重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白对幼年特发性关节炎患儿外周血关节液软骨寡聚基质蛋白表达的影响

    Institute of Scientific and Technical Information of China (English)

    狄亚珍; 吴菱; 王天波; 郑吉卡; 戴霞华

    2014-01-01

    groups was obviously lower than that in the normal control group.The variation was statistically significant (F=25.345,P<0.05).The comparison between any two groups was statistically significant (P<0.05).② The COMP level in the synovial fluid before treatment were (14.8±1.6) ng/ml in the JIA group,(15.1±1.0) ng/ml in the traumatic arthritis group.The variation was not stati-stically significant (t=0.523,P=0.606).③ The serum COMP level of the systemic JIA group was obviously lower than that of the oligoarticular JIA patients,and patients with enthesitis-related arthritis and polyarticular JIA (0.26± 0.03 vs.0.87±0.17,0.89±0.22 and 0.70±0.35 ng/ml,respectively; F=9.244,P<0.05).④ The serum COMP level of JIA at the acute phase was negatively correlated with white blood cells count (WBC),CRP and ESR (r=-0.556,-0.582 and-0.684,respectively; P all<0.05).By contrast,no correlation was detected between the serum COMP level and joint tenderness index,joint swelling index,morning stiffness duration,hemoglobin level and platelet count(r=0.06,-0.206,-0.107,0.15 and-0.185,respectively; P all >0.05).⑤ The serum COMP level was obviously lower in the JIA with joint destruction than that without joint destruction (0.52±0.22 vs.0.92±0.22 ng/ml; t=5.207,P<0.05).⑥After treatment,the blood COMP level in group A was (1.33±0.21) ng/ml and (0.96±0.22) ng/ml in group B,which was obviously higher than that in the JIA group before treatment (0.77±0.29) ng/ml.In addition,the level in group A was higher than that in group B.The variation was statistically significant (F=24.681,P<0.05).⑦ After treatment,the COMP level in the synovial fluid (18.4± 1.1) ng/ml (n=5) was higher than that before the treatment was (14.8± 1.6) ng/ml (n =15).The variation was of statistical significant (t=4.565,P<0.05).Conclusion The COMP level in blood and synovial fluid declines before treatment and increases after treatment.The increase is more obvious after combined with rh

  9. Modelling of the Blood Coagulation Cascade in an In Vitro Flow System

    DEFF Research Database (Denmark)

    Andersen, Nina Marianne; Sørensen, Mads Peter; Efendiev, Messoud A.;

    2010-01-01

    We derive a mathematical model of a part of the blood coagulation cascade set up in a perfusion experiment. Our purpose is to simulate the influence of blood flow and diffusion on the blood coagulation pathway. The resulting model consists of a system of partial differential equations taking into...... and flow equations, which guarantee non negative concentrations at all times. The criteria is applied to the model of the blood coagulation cascade.......We derive a mathematical model of a part of the blood coagulation cascade set up in a perfusion experiment. Our purpose is to simulate the influence of blood flow and diffusion on the blood coagulation pathway. The resulting model consists of a system of partial differential equations taking...

  10. Duffy blood group system and the malaria adaptation process in humans

    OpenAIRE

    Gledson Barbosa de Carvalho; Glauber Barbosa de Carvalho

    2011-01-01

    Malaria is an acute infectious disease caused by the protozoa of the genus Plasmodium. The antigens of the Duffy Blood Group System, in addition to incompatibilities in transfusions and hemolytic disease of the newborn, are of great interest in medicine due to their association with the invasion of red blood cells by the parasite Plasmodium vivax. For invasions to occur an interaction between the parasites and antigens of the Duffy Blood Group System is necessary. In Caucasians six antigens a...

  11. Distribution and clinal trends of the ABO and Rh genes in select Middle Eastern countries.

    Science.gov (United States)

    AlSuhaibani, E S; Kizilbash, N A; Afshan, K; Malik, S

    2015-09-09

    An understanding of the ABO and Rh blood group systems is important for blood transfusions and is also pertinent due to their potential association with certain morbidities and susceptibilities to infections. To investigate the diversity and differentiation of the ABO and Rh loci in Middle Eastern populations, data from twelve representative Middle Eastern populations were analyzed. Six populations were in conformity with Hardy-Weinberg equilibrium at the ABO locus. The pooled heterozygosity at both loci was calculated to be highest in the sample from Jordan and lowest in Bahrain. Heterogeneity was pronounced in the Northern compared to the Southern Middle Eastern populations. Overall, the absolute gene diversity was 0.0046 and gene differentiation was calculated to be 0.0100. Genetic diversity of the studied loci across all populations (HT) was estimated to be 0.4594, while the diversity within the populations (HS) was 0.4548. Nei's genetic distance analyses revealed highest affinities between the populations of Kuwait and Qatar, Oman and Yemen, and between Qatar and the United Arab Emirates. These results were displayed through a UGPMA dendrogram and principal component analyses, which established clustering of certain populations. Clinal trends of the allelic systems were observed by generating contour maps that allow a detailed appreciation of the distributions of alleles across the geography of the Arabian Peninsula and the Middle East. Taken together, these analyses are helpful in understanding the differentiation of blood group loci and for designing prospective studies for establishing the associations of these loci with health variables in the populations studied.

  12. Evaluation of day-by-day variability of home blood pressure using a home blood pressure telemonitoring system.

    Science.gov (United States)

    Kaihara, Toshiki; Eguchi, Kazuo; Hoshide, Satoshi; Kario, Kazuomi

    2016-06-01

    There have been no reports indicating how a home blood pressure (HBP) telemonitoring system influences day-by-day HBP variability. We evaluated day-by-day HBP variability using an HBP telemonitoring system, randomizing 60 individuals to either a telemonitoring group or the control group. There were no significant changes in day-by-day HBP variability between the two groups. However, the SD, root mean sum square differences, and the average real variability values for systolic blood pressure only in the control group in the latter half of the 4-week study were significantly greater than those in the first half (Ptelemonitoring system could be useful in obtaining stable HBP readings over a certain period of time.

  13. [Realization of mobile phone based wireless blood pressure monitoring system and its preliminary implementation].

    Science.gov (United States)

    Wang, Shuo; Gong, Eenhao; Yu, Yang; Liu, Jing

    2011-11-01

    A novel system which could measure blood pressure wirelessly through mobile phone has been proposed and developed. It consisted of a blood pressure signal recorder based on MCU (Micro Control Unit), which could transmit measured data via Bluetooth, and a software embedded on mobile phone, which could calculate the value of blood pressure and the rate of heart beat. The measurement results could easily be stored in text documents or in picture format. Detailed scheme and design of each module in the system was illustrated and experiments were conducted to demonstrate the reliability and stability of the device. A series of new conceptual explorations into blood pressure related issues were conducted with this system, including the variational blood pressure at different time periods in a day, the effect of posture on the measurement results and the impact of movements on blood pressure.

  14. Aloinmunización a un antígeno del sistema Rh de alta frecuencia Alloimmunization to a high frequency Rh antigen

    Directory of Open Access Journals (Sweden)

    Carlos Cotorruelo

    2006-02-01

    Full Text Available Describimos el caso de una embarazada sensibilizada con un aloanticuerpo anti-Rh17 de muy amplia reactividad. Los glóbulos rojos de la paciente presentaban una deleción parcial de los antígenos del sistema Rh, responsable de la aloinmunización encontrada. Debido a la dificultad de obtener sangre compatible se elaboró un plan de transfusión autóloga para cubrir las posibles demandas. El análisis molecular del locus RH demostró la presencia de un alelo híbrido RHCE-D(5-7-CE que generaba el fenotipo delecionado.We report the case of a pregnant woman sensitized with a panreactive anti-Rh17 alloantibody. Patient’s red blood cells showed a partial deletion of Rh antigens, which was responsible for the alloimmunization. An autotransfusion program was instrumented so as to cover possible demands. Molecular analysis of the RH locus showed the presence of a hybrid RHCE-D(5-7-CE allele that gave origin to the deleted phenotype.

  15. Bioavailability of orally administered rhGM-CSF: a single-dose, randomized, open-label, two-period crossover trial.

    Directory of Open Access Journals (Sweden)

    Wenping Zhang

    Full Text Available BACKGROUND: Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF is usually administered by injection, and its oral administration in a clinical setting has been not yet reported. Here we demonstrate the bioavailability of orally administered rhGM-CSF in healthy volunteers. The rhGM-CSF was expressed in Bombyx mori expression system (BmrhGM-CSF. METHODS AND FINDINGS: Using a single-dose, randomized, open-label, two-period crossover clinical trial design, 19 healthy volunteers were orally administered with BmrhGM-CSF (8 microg/kg and subcutaneously injected with rhGM-CSF (3.75 microg/kg respectively. Serum samples were drawn at 0.0h, 0.5h ,0.75h,1.0h,1.5h,2.0h ,3.0h,4.0h,5.0h,6.0h,8.0h,10.0h and 12.0h after administrations. The hGM-CSF serum concentrations were determined by ELISA. The AUC was calculated using the trapezoid method. The relative bioavailability of BmrhGM-CSF was determined according to the AUC ratio of both orally administered and subcutaneously injected rhGM-CSF. Three volunteers were randomly selected from 15 orally administrated subjects with ELISA detectable values. Their serum samples at the 0.0h, 1.0h, 2.0h, 3.0h and 4.0h after the administrations were analyzed by Q-Trap MS/MS TOF. The different peaks were revealed by the spectrogram profile comparison of the 1.0h, 2.0h, 3.0h and 4.0h samples with that of the 0.0h sample, and further analyzed using both Enhanced Product Ion (EPI scanning and Peptide Mass Fingerprinting Analysis. The rhGM-CSF was detected in the serum samples from 15 of 19 volunteers administrated with BmrhGM-CSF. Its bioavailability was observed at an average of 1.0%, with the highest of 3.1%. The rhGM-CSF peptide sequences in the serum samples were detected by MS analysis, and their sizes ranging from 2,039 to 7,336 Da. CONCLUSIONS: The results demonstrated that the oral administered BmrhGM-CSF was absorbed into the blood. This study provides an approach for an oral administration of

  16. [ABO system blood group ratios in patients with neuroinfections].

    Science.gov (United States)

    Rudometov, Iu P; Umanskiĭ, K G; Ashmarina, E E; Andreeva, L S

    1981-01-01

    The distribution of the ABO blood groups in 2009 patients including 1441 ones suffering from etiologically diverse neuroinfections was studied. Certain correlations between the nosological forms and groups of the diseases on the one hand, and the blood factors on the other are demonstrated. The data obtained point to a certain role of hereditary predisposition in the genesis of the neuroinfections. This predisposition predetermines the risk of the illnesses and the gravity of their course, the fact, which is of a practical importance for the clinician.

  17. ABO blood group system and gastric cancer: a case-control study and meta-analysis.

    Science.gov (United States)

    Wang, Zhiwei; Liu, Lei; Ji, Jun; Zhang, Jianian; Yan, Min; Zhang, Jun; Liu, Bingya; Zhu, Zhenggang; Yu, Yingyan

    2012-10-17

    This study focuses on the association between the ABO blood group system and the risk of gastric cancer or Helicobacter pylori infection. The data for the ABO blood group was collected from 1045 cases of gastric cancer, whereby the patient underwent a gastrectomy in Ruijin Hospital, Shanghai. The information on the ABO blood group from 53,026 healthy blood donors was enrolled as control. We searched the Pubmed database on the relationship between ABO blood groups and gastric cancer risk for meta-analysis. In our case-control study, the risk of gastric cancer in blood group A was significantly higher than that in non-A groups (O, B and AB) (odd ratio, OR1.34; 95% confidential interval, CI 1.25-1.44). Compared with non-O groups (A, B and AB), individuals with blood group O demonstrated a reduced risk of gastric cancer (OR = 0.80; 95% CI 0.72-0.88). The proportion of H. pylori infection in blood group A individuals was significantly higher than that in non-A blood groups (OR = 1.42; 95% CI 1.05-1.93). We further combined our data with the published data of others, and crossreferenced the risk of gastric cancer with the blood type, finding consistent evidence that gastric cancer risk in the blood A group was higher than that in the non-A groups (OR = 1.11; 95% CI 1.07-1.15), and that blood type O individuals were consistently shown gastric cancer risk reduction (OR = 0.91; 95% CI 0.89-0.94). Our study concluded that there was a slightly increased risk of gastric cancer in blood group A individuals, and people with blood type A are more prone to be infected by H. pylori than other ABO blood type individuals, whereas, a slightly decreased risk of gastric cancer was identified in blood type O individuals.

  18. Dimethylphosphinate bridged binuclear Rh(i) catalysts for the alkoxycarbonylation of aromatic C-H bonds.

    Science.gov (United States)

    Iturmendi, Amaia; Sanz Miguel, Pablo J; Popoola, Saheed A; Al-Saadi, Abdulaziz A; Iglesias, Manuel; Oro, Luis A

    2016-11-14

    A variety of binuclear rhodium(i) complexes featuring two bridging dimethylphosphinate ligands ((CH3)2PO2(-)) have been prepared and tested in the alkoxycarbonylation of aromatic C-H bonds. The complex [Rh(μ-κO,O'-(CH3)2PO2)(cod)]2 has been prepared by a reaction of [Rh(μ-MeO)(cod)]2 with 2 equivalents of dimethylphosphinic acid. Binuclear complexes [Rh(μ-κO,O'-(CH3)2PO2)(CO)L]2 (L = PPh3, P(OMe)Ph2 and P(OPh)3) were obtained by carbonylation of the related mononuclear complexes [Rh(κO-(CH3)2PO2)(cod)(L)], which were prepared in situ by the reaction of [Rh(μ-κO,O'-(CH3)2PO2)(cod)]2 with 2 equivalents of L. Conversely, if L = IPr, the reaction of [Rh(μ-κO,O'-(CH3)2PO2)(CO)L]2 with carbon monoxide affords the mononuclear complex [Rh(κO-(CH3)2PO2)(CO)2IPr]. The subsequent reaction with trimethylamine N-oxide gives the corresponding binuclear complex [Rh(μ-κO,O'-(CH3)2PO2)(CO)(IPr)]2 by abstraction of one of the carbonyl ligands. Complexes [Rh(μ-κO,O'-(CH3)2PO2)(cod)]2 and [Rh(κO-(CH3)2PO2)(cod)(L)] (L = IPr, PPh3, P(OMe)Ph2, P(OPh)3) are active precatalysts in the alkoxycarbonylation of C-H bonds, with the ligand system playing a key role in the catalytic activity. The complexes that feature more labile Rh-L bonds give rise to better catalysts, probably due to the more straightforward substitution of L by a second carbonyl ligand, since a more electrophilic carbonyl carbon atom is more susceptible toward aryl migration. In fact, complexes [Rh(μ-κO,O'-(CH3)2PO2)(CO)2]2 and [Rh(μ-Cl)(CO)2]2, generated in situ from [Rh(μ-κO,O'-(CH3)2PO2)(cod)]2 and [Rh(μ-Cl)(cod)2]2, respectively, are the most active catalysts tested in this work.

  19. GnRH-agonist versus GnRH-antagonist IVF cycles

    DEFF Research Database (Denmark)

    Papanikolaou, E G; Pados, G; Grimbizis, G

    2012-01-01

    In view of the current debate concerning possible differences in efficacy between the two GnRH analogues used in IVF stimulated cycles, the current study aimed to explore whether progesterone control in the late follicular phase differs when GnRH antagonist is used as compared with GnRH agonist...

  20. Rh Variability in Multi-Ethnic Perspective: Consequences for RH Genotyping

    NARCIS (Netherlands)

    G.H.M. Tax

    2006-01-01

    textabstractThe RhD bloodgroup was first described by Levine en Stetson in 1939 after the manifestation of a hemolytic transfusion reaction in a woman who recently gave birth, after transfusion with her husbands red cells. The RhD-negative woman produced antibodies against the RhD present on the r

  1. An isolated nitridyl radical-bridged {Rh(N.)Rh} complex

    NARCIS (Netherlands)

    Gloaguen, Yann; Rebreyend, Christophe; Lutz, Martin|info:eu-repo/dai/nl/304828971; Kumar, Pravin; Huber, Martina; Vandervlugt, Jarl Ivar; Schneider, Sven; Debruin, Bas

    2014-01-01

    Photochemical activation of [(PNNH)Rh(N3)] (PNNH=6-di-(tert- butyl)phosphinomethyl-2,2′-bipyridine) complex2 produced the paramagnetic (S=1/2), [(PNN)Rh-N.-Rh(PNN)] complex3 (PNN-=methylene- deprotonated PNNH), which could be crystallographically characterized. Spectroscopic investigation of 3

  2. Rh Variability in Multi-Ethnic Perspective: Consequences for RH Genotyping

    NARCIS (Netherlands)

    G.H.M. Tax

    2006-01-01

    textabstractThe RhD bloodgroup was first described by Levine en Stetson in 1939 after the manifestation of a hemolytic transfusion reaction in a woman who recently gave birth, after transfusion with her husbands red cells. The RhD-negative woman produced antibodies against the RhD present on the

  3. [Fetomaternal anti-RH3, -4 (anti-E and anti-c) rhesus isoimmunization: a case report].

    Science.gov (United States)

    Farnault, L; Garcia-Meric, P; Cortey, A; Arnaud, F

    2011-02-01

    Hemolytic disease of the newborn caused by maternal isoimmunization has been decreasing over the past 10 years because of prophylactic treatment with anti-RH1 (anti-D) immunoglobulin. Nevertheless, there is an increase in the incidence of both relative and absolute numbers of non-RH1 red-cell maternofetal isoimmunizations, essentially anti-RH4 (anti-c), anti-RH3 (anti-E), and anti-Kell. In 8 to 14% of cases, multispecificity antibodies are present, the most common combination being the association of anti-RH3 and -4. Despite absence of specific prophylactic therapy, anti-RH4 isoimmunization could be as severe as anti-RH1 ; as for anti-RH3, it is usually associated with mild to moderate clinical manifestations. Nevertheless, there are few publications on anti-RH3, -4 maternofetal isoimmunization with a bias toward the most severe cases being reported. We report here a case of nonsevere maternofetal anti-RH3, -4 isoimmunization complicated with severe hyperbilirubinemia and delayed profound anemia. Hyperbilirubinemia was controlled using intensive phototherapy. Although anemia was absent at birth, it appeared progressively with a nadir at 7.8 g/dL at 1-month postnatal age. Blood counts were monitored for 3 months but the patient did not require red blood cell transfusion. This report underlines the need for a prolonged and rigorous pediatric follow-up of children born in the context of maternofetal isoimmunization after the acute neonatal period. Furthermore, it stresses the necessity of DAT testing in all pregnant women, even those who are RH1-positive.

  4. Water/Oil Biphasic Hydroformylation of Higher Olefins over a TPPTS-Rh/SiO2 Catalyst

    Institute of Scientific and Technical Information of China (English)

    Hejun Zhu; Yunjie Ding; Fu Yang; Li Yan; Jianmin Xiong; Hongmei Yin; Liwu Lin

    2004-01-01

    A novel TPPTS-Rh/SiO2 catalyst, prepared by directly modifying a heterogeneous highsurface-area Rh/SiO2 catalyst with water-soluble TPPTS ligands, could decrease the resistance of mass transfer in water/oil biphasic media for the hydroformylation of higher olefins. The catalytic performance for hydroformylation on this biphasic TPPTS-Rh/SiO2 catalyst system was higher than those of the traditional biphasic HRhCO(TPPTS)3 systems, owing to the chemical bonds between the highly dispersed Rh metal particles and the TPPTS ligands. The catalyst system is applicable for hydroformylation of higher olefins such as 1-dodecene.

  5. Enhancing blockade of Plasmodium falciparum erythrocyte invasion: assessing combinations of antibodies against PfRH5 and other merozoite antigens.

    Directory of Open Access Journals (Sweden)

    Andrew R Williams

    Full Text Available No vaccine has yet proven effective against the blood-stages of Plasmodium falciparum, which cause the symptoms and severe manifestations of malaria. We recently found that PfRH5, a P. falciparum-specific protein expressed in merozoites, is efficiently targeted by broadly-neutralizing, vaccine-induced antibodies. Here we show that antibodies against PfRH5 efficiently inhibit the in vitro growth of short-term-adapted parasite isolates from Cambodia, and that the EC(50 values of antigen-specific antibodies against PfRH5 are lower than those against PfAMA1. Since antibody responses elicited by multiple antigens are speculated to improve the efficacy of blood-stage vaccines, we conducted detailed assessments of parasite growth inhibition by antibodies against PfRH5 in combination with antibodies against seven other merozoite antigens. We found that antibodies against PfRH5 act synergistically with antibodies against certain other merozoite antigens, most notably with antibodies against other erythrocyte-binding antigens such as PfRH4, to inhibit the growth of a homologous P. falciparum clone. A combination of antibodies against PfRH4 and basigin, the erythrocyte receptor for PfRH5, also potently inhibited parasite growth. This methodology provides the first quantitative evidence that polyclonal vaccine-induced antibodies can act synergistically against P. falciparum antigens and should help to guide the rational development of future multi-antigen vaccines.

  6. ACTIVE TARGETING WITH PARTICULATE CARRIER SYSTEMS IN THE BLOOD COMPARTMENT

    NARCIS (Netherlands)

    CROMMELIN, DJA; SCHERPHOF, G; STORM, G

    1995-01-01

    This review deals with active targeting of particulate drug carriers through (1) physico-chemical (e.g., complex formation between a homing device and a surface exposed molecule at the target site) and (2) physical means, Target sites discussed are restricted to those in the blood circulation. Targe

  7. Differential Rheology Among ABO Blood Group System In Nigerians

    African Journals Online (AJOL)

    Official Publication of the African Association of Physiological Sciences ... alterations in membrane and cytoskeletal properties that could affect the rheology of blood. This study was therefore .... microcirculation and acute organ dysfunction (Oguz and Herbert ..... Ajayi et al. Robert E. and Marx D.D.S. (2001): Platelet-Rich.

  8. Fgf8-deficient mice compensate for reduced GnRH neuronal population and exhibit normal testicular function

    Directory of Open Access Journals (Sweden)

    Wei eZhang

    2015-09-01

    Full Text Available Gonadotropin-releasing hormone (GnRH is critical for the onset and maintenance of reproduction in vertebrates. The development of GnRH neurons is highly dependent on fibroblast growth factor (Fgf signaling. Mice with a hypomorphic Fgf8 allele (Fgf8 Het exhibited a ~50% reduction in GnRH neuron number at birth. Female Fgf8 Het mice were fertile but showed significantly delayed puberty. However, it was unclear if these mice suffered additional loss of GnRH neurons after birth, and if male Fgf8 Het mice had normal pubertal transition and testicular function. In this study, we examined postnatal GnRH neuron number and hypothalamic GnRH content in Fgf8 Het mice from birth to 120 days of age. Further, we examined seminal vesicle and testicular growth, testicular histology, and circulating luteinizing hormone (LH around and after pubertal transition. Our results showed that GnRH neuron numbers were significantly and consistently reduced in Fgf8 Het mice of both sexes in all ages examined, suggesting these animals were born with an inherently defective GnRH system, and no further postnatal loss of GnRH neurons had occurred. Despite an innately compromised GnRH system, male and female Fgf8 mice exhibited normal levels of immunoassayable hypothalamic GnRH peptide at all ages examined except on 60 days of age, suggesting increased GnRH synthesis or reduced turnover as a compensatory mechanism. Fgf8 Het males also had normal seminal vesicle and testicular mass/body mass ratios, testicular histology, and circulating LH. Overall, our data speak to the extraordinary ability of a GnRH system permanently compromised by developmental defect to overcome pre-existing deficiencies to ensure pubertal progression and reproduction.

  9. 南阳市Rh D阴性稀有血型库的建立及临床意义

    Institute of Scientific and Technical Information of China (English)

    凌励

    2015-01-01

    Objective The establishment of a rare blood bank in Nanyang, and to explore its clinical signifi cance.Methods Establish Nanyang city rare blood type library retrieval, and explore the clinical signifi cance of the micro plate method of Nanyang City 81462 blood donation, Rh blood group antigen D for screening and Rh D negative samples and then at the beginning of the blood group screen with three different batches of IgG anti-D antibodies of anti human globulin (IAT) experiment validation. Initial screening and confi rmation tests were negative for D Rh negative blood group. D ABO negative Rh blood group and Rh blood group were also examined.Results All blood donors were screened and confi rmed that a total of 300 D Rh negative blood group, accounting for 0.37%, of which the type B blood is the most, the performance of ccdee based. The data of blood donors and the results of the examination were entered into the computer, the establishment of the Nanyang area D Rh negative rare blood group information database.Conclusion Rare blood group RH D negative information database for emergency treatment of RH D negative patients with blood provides a green life path, to prevent transfusion of Rh blood group incompatibility caused by acute hemolytic reaction and Rh blood group incompatibility of pregnancy induced hemolytic disease of the newborn is of far-reaching signifi cance.%目的:建立南阳市稀有血型检索库,并探讨其临床意义。方法采用微板法对南阳市81462名无偿献血者Rh D血型抗原进行初筛检查,对血型初筛Rh D阴性标本再用3个不同批号的IgG抗-D抗体试剂进行抗人球蛋白(IAT)实验确认。初筛和确认实验均为阴性者为Rh D阴性血型。同时检查Rh D阴性者的ABO血型以及Rh血型的表现型。结果所有献血者经筛选和确认共有300名Rh D阴性血型,占0.37%,其中B型血最多,表现型以ccdee为主。将献血者的资料和检查的结果输入计算机,建立南阳地区Rh

  10. A better anti-diabetic recombinant human fibroblast growth factor 21 (rhFGF21 modified with polyethylene glycol.

    Directory of Open Access Journals (Sweden)

    Zhifeng Huang

    Full Text Available As one of fibroblast growth factor (FGF family members, FGF21 has been extensively investigated for its potential as a drug candidate to combat metabolic diseases. In the present study, recombinant human FGF21 (rhFGF21 was modified with polyethylene glycol (PEGylation in order to increase its in vivo biostabilities and therapeutic potency. At N-terminal residue rhFGF21 was site-selectively PEGylated with mPEG20 kDa-butyraldehyde. The PEGylated rhFGF21 was purified to near homogeneity by Q Sepharose anion-exchange chromatography. The general structural and biochemical features as well as anti-diabetic effects of PEGylated rhFGF21 in a type 2 diabetic rat model were evaluated. By N-terminal sequencing and MALDI-TOF mass spectrometry, we confirmed that PEG molecule was conjugated only to the N-terminus of rhFGF21. The mono-PEGylated rhFGF21 retained the secondary structure, consistent with the native rhFGF21, but its biostabilities, including the resistance to physiological temperature and trypsinization, were significantly enhanced. The in vivo immunogenicity of PEGylated rhFGF21 was significantly decreased, and in vivo half-life time was significantly elongated. Compared to the native form, the PEGylated rhFGF21 had a similar capacity of stimulating glucose uptake in 3T3-L1 cells in vitro, but afforded a significantly long effect on reducing blood glucose and triglyceride levels in the type 2 diabetic animals. These results suggest that the PEGylated rhFGF21 is a better and more effective anti-diabetic drug candidate than the native rhFGF21 currently available. Therefore, the PEGylated rhFGF21 may be potentially applied in clinics to improve the metabolic syndrome for type 2 diabetic patients.

  11. Ginsenoside Rh2 Improves Cardiac Fibrosis via PPARδ–STAT3 Signaling in Type 1-Like Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Shih-Hsiang Lo

    2017-06-01

    Full Text Available Ginsenoside Rh2 (Rh2 is an active principal ingredient contained in ginseng (Panax ginseng Meyer, a medicinal herb used to enhance health worldwide. The present study is designed to investigate the effect of Rh2 on myocardial fibrosis in diabetic rats. In a streptozotocin-induced model of type-1 diabetic rats (STZ-diabetic rats, the increased fasting blood glucose levels and heart weight/body weight (HW/BW ratio were substantially alleviated by Rh2. Moreover, Rh2 improved cardiac performance in STZ-diabetic rats. Histological results from Masson staining showed that Rh2 attenuated cardiac fibrosis in STZ-diabetic rats. The effects of Rh2 were reversed by GSK0660 at a dose sufficient to inhibit peroxisome proliferator-activated receptor δ (PPARδ in STZ-diabetic rats. The role of PPARδ was subsequently investigated in vitro. Rh2 restored the decreased PPARδ expression level in high glucose-cultured cardiomyocytes. Moreover, increased protein levels of fibrotic signals, including signal transducer and activator of transcription 3 (STAT3, connective tissue growth factor (CCN2 and fibronectin, were reduced by Rh2 in high glucose-cultured cardiomyocytes. These effects of Rh2 were reversed by GSK0660 or siRNA specific for PPARδ Taken together, PPARδ activation may inhibit STAT3 activation to reduce CCN2 and fibronectin expression in diabetic rats with cardiac fibrosis. Moreover, Rh2 improves cardiac function and fibrosis by increasing PPARδ signaling. Therefore, Rh2 is suitable to develop as an alternative remedy for cardiac fibrosis.

  12. Exchange coupling in metallic multilayers with a top FeRh layer

    Science.gov (United States)

    Yamada, S.; Tanikawa, K.; Hirayama, J.; Kanashima, T.; Taniyama, T.; Hamaya, K.

    2016-05-01

    We study magnetic properties of metallic multilayers with FeRh/ferromagnet interfaces grown by low-temperature molecular beam epitaxy. Room-temperature coercivity of the ferromagnetic layers is significantly enhanced after the growth of FeRh, proving the existence of the exchange coupling between the antiferromagnetic FeRh layer and the ferromagnetic layer. However, exchange bias is not clearly observed probably due to the presence of disordered structures, which result from the lattice strain at the FeRh/ferromagnet interfaces due to the lattice mismatch. We infer that the lattice matched interface between FeRh and ferromagnetic layers is a key parameter for controlling magnetic switching fields in such multilayer systems.

  13. Local structural evidence for strong electronic correlations in spinel LiRh2O4

    Science.gov (United States)

    Knox, K. R.; Abeykoon, A. M. M.; Zheng, H.; Yin, W.-G.; Tsvelik, A. M.; Mitchell, J. F.; Billinge, S. J. L.; Bozin, E. S.

    2013-11-01

    The local structure of the spinel LiRh2O4 has been studied using atomic-pair distribution function analysis of powder x-ray diffraction data. This measurement is sensitive to the presence of short Rh-Rh bonds that form due to dimerization of Rh4+ ions on the pyrochlore sublattice, independent of the existence of long-range order. We show that structural dimers exist in the low-temperature phase, as previously supposed, with a bond shortening of Δr˜0.15 Å. The dimers persist up to 350 K, well above the insulator-metal transition, with Δr decreasing in magnitude on warming. Such behavior is inconsistent with the Fermi-surface nesting-driven Peierls transition model. Instead, we argue that LiRh2O4 should properly be described as a strongly correlated system.

  14. Valence states and possible charge ordering in LaCo1-x Rh x O3

    Science.gov (United States)

    Streltsov, Sergey V.; Gapontsev, Vladimir V.; Khomskii, Daniel I.

    2016-03-01

    An unusual effect was discovered in Li et al (2010 J. Solid State Chem. 183 1388): the substitution of nonmagnetic low-spin Co3+ in LaCoO3 by the formally isoelectronic and also nonmagnetic Rh3+ led, surprisingly, to a rapid appearance of magnetism in LaCo1-x Rh x O3, even for small amounts of doping. Different explanations for this effect were proposed in the literature. To clarify the situation we carried out unbiased ab initio calculations of this system. We concluded that, in agreement with the original assumption of Li et al, but in contrast with later statements (Knizek et al 2012 Phys. Rev. B 85 134401), this effect is caused by the valence change (’redox reaction’) Co3+   +  Rh3+ \\to Co2+   +  Rh4+ , which creates magnetic Co2+ and Rh4+ ions. For the half-filled case LaCo1/2Rh1/2O3 we obtained the state with charge ordering of Co2+ and Rh4+ ions, which according to our calculations are antiferromagnetically coupled. The obtained results reasonably explain the observed behavior of the magnetic susceptibility of LaCo1-x Rh x O3, and the novel state predicted at half-doping could be verified experimentally by detailed structural and magnetic studies and by x-ray absorption spectroscopy.

  15. Valence states and possible charge ordering in LaCo(1-x)Rh(x)O₃.

    Science.gov (United States)

    Streltsov, Sergey V; Gapontsev, Vladimir V; Khomskii, Daniel I

    2016-03-02

    An unusual effect was discovered in Li et al (2010 J. Solid State Chem. 183 1388): the substitution of nonmagnetic low-spin Co(3+) in LaCoO3 by the formally isoelectronic and also nonmagnetic Rh(3+) led, surprisingly, to a rapid appearance of magnetism in LaCo(1-x)Rh(x)O3, even for small amounts of doping. Different explanations for this effect were proposed in the literature. To clarify the situation we carried out unbiased ab initio calculations of this system. We concluded that, in agreement with the original assumption of Li et al, but in contrast with later statements (Knizek et al 2012 Phys. Rev. B 85 134401), this effect is caused by the valence change ('redox reaction') Co(3+) +  Rh(3+) → Co(2+) +  Rh(4+), which creates magnetic Co(2+) and Rh(4+) ions. For the half-filled case LaCo1/2Rh1/2O3 we obtained the state with charge ordering of Co(2+) and Rh(4+) ions, which according to our calculations are antiferromagnetically coupled. The obtained results reasonably explain the observed behavior of the magnetic susceptibility of LaCo(1-x)Rh(x)O3, and the novel state predicted at half-doping could be verified experimentally by detailed structural and magnetic studies and by x-ray absorption spectroscopy.

  16. Partial phenotyping in voluntary blood donors of Gujarat State

    Directory of Open Access Journals (Sweden)

    Maitrey Gajjar

    2016-01-01

    Full Text Available Introduction: Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues.The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better planning and management of blood bank; the main goal is to find compatible blood for patients needing multiple blood transfusions. The aim of this study was to evaluate the frequency of Rh & Kell phenotype of voluntary donors in Gujarat state. Materials and Methods: The present study was conducted by taking 5670 samples from random voluntary blood donors coming in blood donation camp. Written consent was taken for donor phenotyping. The antigen typing of donors was performed by Qwalys-3(manufacturer: Diagast by using electromagnetic technology on Duolys plates. Results: Out of 5670 donors, the most common Rh antigen observed in the study population was e (99.07% followed by D (95.40%, C (88.77%, c (55.89% and E (17.88%. The frequency of the Kell antigen (K was 1.78 %. Discussion: The antigen frequencies among blood donors from Gujarat were compared with those published for other Indian populations. The frequency of D antigen in our study (95.4% and north Indian donors (93.6 was significantly higher than in the Caucasians (85% and lower than in the Chinese (99%. The frequencies of C, c and E antigens were dissimilar to other ethnic groups while the ′e′ antigen was present in high frequency in our study as also in the other ethnic groups. Kell antigen (K was found in only 101 (1.78 % donors out of 5670. Frequency of Kell antigen in Caucasian and Black populations is 9% & 2% respectively. The most common Kell phenotype was K-k+, not just in Indians (96.5% but

  17. Partial phenotyping in voluntary blood donors of Gujarat State.

    Science.gov (United States)

    Gajjar, Maitrey; Patel, Tarak; Bhatnagar, Nidhi; Patel, Kruti; Shah, Mamta; Prajapati, Amit

    2016-01-01

    Partial phenotyping of voluntary blood donors has vital role in transfusion practice, population genetic study and in resolving legal issues. The Rh blood group is one of the most complex and highly immunogenic blood group known in humans. The Kell system, discovered in 1946, is the third most potent system at triggering hemolytic transfusion reactions and consists of 25 highly immunogenic antigens. Knowledge of Rh & Kell phenotypes in given population is relevant for better planning and management of blood bank; the main goal is to find compatible blood for patients needing multiple blood transfusions. The aim of this study was to evaluate the frequency of Rh & Kell phenotype of voluntary donors in Gujarat state. The present study was conducted by taking 5670 samples from random voluntary blood donors coming in blood donation camp. Written consent was taken for donor phenotyping. The antigen typing of donors was performed by Qwalys-3(manufacturer: Diagast) by using electromagnetic technology on Duolys plates. Out of 5670 donors, the most common Rh antigen observed in the study population was e (99.07%) followed by D (95.40%), C (88.77%), c (55.89%) and E (17.88%). The frequency of the Kell antigen (K) was 1.78 %. The antigen frequencies among blood donors from Gujarat were compared with those published for other Indian populations. The frequency of D antigen in our study (95.4%) and north Indian donors (93.6) was significantly higher than in the Caucasians (85%) and lower than in the Chinese (99%). The frequencies of C, c and E antigens were dissimilar to other ethnic groups while the 'e' antigen was present in high frequency in our study as also in the other ethnic groups. Kell antigen (K) was found in only 101 (1.78 %) donors out of 5670. Frequency of Kell antigen in Caucasian and Black populations is 9% & 2% respectively. The most common Kell phenotype was K-k+, not just in Indians (96.5%) but also in Caucasians (91%), Blacks (98%) and Chinese (100%). Phenotype

  18. [Blood system peculiarities in the bank vole (Clethrionomys glareolus) under chronic environmental pollution].

    Science.gov (United States)

    Tarakhtiĭ, É A; Mukhacheva, S V

    2011-01-01

    The parameters of peripheral blood and hemopoietic organs in mature and immature bank voles inhabiting a chemically polluted area were studied. Variability of the blood system parameters depending on the level of toxic load and the animals' reproductive status was determined. Alteration of the cell composition of erythrocytes and leucocytes, the structure of erythrocytes, and the hemoglobin fractions and leucocyte functions describe the adaptive response to the factors of a changed environment more than the concentration of leucocytes, erythrocytes, and blood hemoglobin.

  19. Rh-catalyzed linear hydroformylation of styrene

    NARCIS (Netherlands)

    Boymans, E.; Janssen, Michèle; Müller, Christian; Lutz, M.|info:eu-repo/dai/nl/304828971; Vogt, D.

    2012-01-01

    Usually the Rh-catalyzed hydroformylation of styrene predominantly yields the branched, chiral aldehyde. An inversion of regioselectivity can be achieved using strong π-acceptor ligands. Binaphthol-based diphosphite and bis(dipyrrolyl-phosphorodiamidite) ligands were applied in the Rh-catalyzed hydr

  20. Evaluation of the Rh(II)-Rh(II) bond dissociation enthalpy for [(TMTAA)Rh]2 by 1H NMR T2 measurements: application in determining the Rh-C(O)- BDE in [(TMTAA)Rh]2C═O.

    Science.gov (United States)

    Imler, Gregory H; Zdilla, Michael J; Wayland, Bradford B

    2013-10-07

    Toluene solutions of the rhodium(II) dimer of dibenzotetramethylaza[14]annulene ([(TMTAA)Rh]2; (1)) manifest an increase in the line widths for the singlet methine and methyl (1)H NMR resonances with increasing temperature that result from the rate of dissociation of the diamagnetic Rh(II)-Rh(II) bonded dimer (1) dissociating into paramagnetic Rh(II) monomers (TMTAA) Rh (2). Temperature dependence of the rates of Rh(II)-Rh(II) dissociation give the activation parameters for bond homolysis ΔH(‡)(app) = 24(1) kcal mol(-1) and ΔS(‡)(app) = 10 (1) cal K(-1) mol(-1) and an estimate for the Rh(II)-Rh(II) bond dissociation enthalpy (BDE) of 22 kcal mol(-1). Thermodynamic values for reaction of 1 with CO to form (TMTAA)Rh-C(O)-Rh(TMTAA) (3) ΔH1° = -14 (1) kcal mol(-1), ΔS1°= -30(3) cal K(-1) mol(-1)) were used in deriving a (TMTAA)Rh-C(O)- BDE of 53 kcal mol(-1).

  1. Imaging of blood cells based on snapshot Hyper-Spectral Imaging systems

    Science.gov (United States)

    Robison, Christopher J.; Kolanko, Christopher; Bourlai, Thirimachos; Dawson, Jeremy M.

    2015-05-01

    Snapshot Hyper-Spectral imaging systems are capable of capturing several spectral bands simultaneously, offering coregistered images of a target. With appropriate optics, these systems are potentially able to image blood cells in vivo as they flow through a vessel, eliminating the need for a blood draw and sample staining. Our group has evaluated the capability of a commercial Snapshot Hyper-Spectral imaging system, the Arrow system from Rebellion Photonics, in differentiating between white and red blood cells on unstained blood smear slides. We evaluated the imaging capabilities of this hyperspectral camera; attached to a microscope at varying objective powers and illumination intensity. Hyperspectral data consisting of 25, 443x313 hyperspectral bands with ~3nm spacing were captured over the range of 419 to 494nm. Open-source hyper-spectral data cube analysis tools, used primarily in Geographic Information Systems (GIS) applications, indicate that white blood cells features are most prominent in the 428-442nm band for blood samples viewed under 20x and 50x magnification over a varying range of illumination intensities. These images could potentially be used in subsequent automated white blood cell segmentation and counting algorithms for performing in vivo white blood cell counting.

  2. GnRH agonist triggering

    DEFF Research Database (Denmark)

    Kol, Shahar; Humaidan, Peter; Al Humaidan, Peter Samir Heskjær

    2013-01-01

    The concept that a bolus of gonadotrophin-releasing hormone agonist (GnRHa) can replace human chorionic gonadotrophin (HCG) as a trigger of final oocyte maturation was introduced several years ago. Recent developments in the area strengthen this premise. GnRHa trigger offers important advantages...... triggering concept should be challenged and that the GnRHa trigger is the way to move forward with thoughtful consideration of the needs, safety and comfort of our patients. Routinely, human chorionic gonadotrophin (HCG) is used to induce ovulation in fertility treatments. This approach deviates...... significantly from physiology and often results in insufficient hormonal support in early pregnancy and in ovarian hyperstimulation syndrome (OHSS). An alternative approach is to use a gonadotrophin-releasing hormone (GnRH) agonist which allows a more physiological trigger of ovulation and, most importantly...

  3. RH ISOIMMUNIZATION BY MULTIPLE ANTIGENS

    OpenAIRE

    2005-01-01

    RESUMO A doença hemolítica perinatal isoimune (DHP) resulta da destruição dos eritrócitos fetais e do recém-nascido por anticorpos maternos dirigidos especificamente contra os antigénios de membrana dessas células. Os autores apresentam um caso de isoimunização Rh a antigénios múltiplos, anti-C, D e E, num 2º filho de um casal com antecedentes de abortamento espontâneo e um 1º filho com DHP anti- CDE e necessidade de transfusão permuta. Apesar da gravidade, com necessidade de várias tra...

  4. Blood flow in the cerebral venous system: modeling and simulation.

    Science.gov (United States)

    Miraucourt, Olivia; Salmon, Stéphanie; Szopos, Marcela; Thiriet, Marc

    2017-04-01

    The development of a software platform incorporating all aspects, from medical imaging data, through three-dimensional reconstruction and suitable meshing, up to simulation of blood flow in patient-specific geometries, is a crucial challenge in biomedical engineering. In the present study, a fully three-dimensional blood flow simulation is carried out through a complete rigid macrovascular circuit, namely the intracranial venous network, instead of a reduced order simulation and partial vascular network. The biomechanical modeling step is carefully analyzed and leads to the description of the flow governed by the dimensionless Navier-Stokes equations for an incompressible viscous fluid. The equations are then numerically solved with a free finite element software using five meshes of a realistic geometry obtained from medical images to prove the feasibility of the pipeline. Some features of the intracranial venous circuit in the supine position such as asymmetric behavior in merging regions are discussed.

  5. Haemolytic activity of Actinobacillus actinomycetemcomitans strains on different blood types Atividade hemolítica de cepas de Actinobacillus actinomycetemcomitans em diferentes tipos sanguíneos

    Directory of Open Access Journals (Sweden)

    Mario Julio Avila-Campos

    1995-06-01

    Full Text Available Haemolytic activity of sixty nine Actinobacillus actinomycetemcomitans strains on different animal and human blood types was examined by using a trypticase soy agar supplemented with yeast extract (0.5%. Blood types used were: rabbit, sheep and human (A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+; AB, Rh- groups. Plates were inoculated and, incubated in microaerophilic conditions, at 37ºC, for 48 h. The haemolytic activity of the tested strains was characterized as alpha-haemolysis. Only two isolates were not haemolytic on all blood types (2.9%, two strains were haemolytic only on human blood (one strain on AB, Rh+ group and another one on A, Rh+ and AB, Rh+ groups. No specificity between haemolysin produced by the tested strains and blood type was observed.A atividade hemolítica de 69 cepas de Actinobacillus actinomycetemcomitans foi determinada em diferentes tipos de sangue animal e humano, usando como meio base ágar de soja tripticaseina, suplementado com extrato de levedura (0,5%. Foram utilizados sangue de coelho, carneiro e humano (grupos A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+ e AB, Rh-. As placas foram inoculadas e, incubadas em condições de microaerofilia, a 37ºC, por 48 h. A atividade hemolítica das cepas testadas foi caracterizada como alfa-hemólise. Somente dois (2,9% isolados não hemolisaram todos os tipos sanguíneos, duas cepas hemolisaram somente sangue humano (uma o grupo AB, Rh+ e outra os grupos A, Rh+ e AB, Rh+. Não foi observada alguma especificidade entre as hemolisinas produzidas e os tipos de sangue utilizados.

  6. Kisspeptin Excitation of GnRH Neurons

    Science.gov (United States)

    Rønnekleiv, Oline K.; Kelly, Martin J.

    2014-01-01

    Kisspeptin binding to its cognate G protein-coupled receptor (GPR54, aka Kiss1R) in gonadotropin-releasing hormone (GnRH) neurons stimulates peptide release and activation of the reproductive axis in mammals. Kisspeptin has pronounced pre- and postsynaptic effects, with the latter dominating the excitability of GnRH neurons. Presynaptically, kisspeptin increases the excitatory drive (both GABA-A and glutamate) to GnRH neurons and postsynaptically, kisspeptin inhibits an A-type and inwardly rectifying K + (Kir 6.2 and GIRK) currents and activates nonselective cation (TRPC) currents to cause long-lasting depolarization and increased action potential firing. The signaling cascades and the multiple intracellular targets of kisspeptin actions in native GnRH neurons are continuing to be elucidated. This review summarizes our current state of knowledge about kisspeptin signaling in GnRH neurons. PMID:23550004

  7. Atherosclerosis of coronary blood vessels - local or systemic inflamation?

    Science.gov (United States)

    Pejkov, Hristo; Kedev, Sasko; Panov, Saso; Srbinovska-Kostovska, Elizabeta; Lang, Irene

    2013-01-01

    The presence of atherosclerotic lesions in the blood vessels is a predisposition for the development and occurrence of acute ischaemic attacks. Bigger atherosclerotic lesions in the coronary blood vessels cause lumen occlusion, which is a cause of acute myocardial infarction. Endothelial dysfunction is defined as an ability of the endothelium to produce vasorelaxing nitric oxide (NO), or deregulation of the other vasoactive substances, such as angiotensin II and endothelin [13]. This definition describes endothelial dysfunction as an improper vasomotor constriction of the vessel, that leads to lumen occlusion of the already existing atherosclerotic lesions. According to the modern model, the development of atherosclerotic plaque and inappropriate endothelial NO production have a synergistic role in patho-physiological and molecular processes in the blood vessels [14]. Lesions in the coronary arteries are deposits of huge quantities of foamy cells and fibrous plaques. The thin fibrous plaques are 10-20% of the total plaque population and are the cause of 80-90% of clinical cases due to their ability to rupture [48]. According to all the results from published studies by far, it has been pointed out that the plaque stability, not the absolute size influences the rupture potential. Elucidating the risk factors that may modify in the atherogenesis and the consequent atherothrombic effect is the first step to this goal.

  8. A decision support system for quantitative measurement of operational efficiency in a blood collection facility.

    Science.gov (United States)

    Kros, John F; Yim Pang, Robyn

    2004-04-01

    A decision support system (DSS) is presented that allows users to input, analyze, and output data derived from blood banking operations. The DSS developed is a hybrid system that is both data and model driven. The system provides information, models, and data manipulation tools to assist users in the quantitative measurement of the operational efficiency in a blood collection facility. A relational database was developed to address the four major variables, which impact the cost per unit of blood being collected. Using visual basic, a user interface and mathematical model were developed establishing the relationships to analyze cost per unit of collected blood. Using inputs from users and historical financial data, the DSS calculates the cost per unit as each of the major variables is altered. Real life situations by the mobile operations team at a blood collection facility were used to test the DSS.

  9. Neonatal Outcomes of Rh-Negative Pregnancies in a Tertiary Level Neonatal Intensive Care Unit: A Prospective Study

    Directory of Open Access Journals (Sweden)

    Chacham

    2016-07-01

    Full Text Available Background Rhesus incompatibility is a preventable cause for severe neonatal hyperbilirubinemia, hydrops fetalis and still births. The prevalence of the Rh-negative blood group among Indian woman varies from 2% - 10%. Despite declining the incidence of Rhesus incompatibility, due to availability of anti-D immunoglobulin, and improved antenatal care of the Rh-negative pregnant woman, it still accounts for a significant proportion of neonatal hyperbilirubinemia and neuro-morbidity. The prevalence of Rh-negative women having Rh-positive neonates is 60%. Objectives This study aimed to estimate the incidence of Rh iso-immunization and evaluate the outcomes of Rh iso-immunized neonates. Methods This prospective observational study was conducted in a tertiary level neonatal intensive care unit, Princess Esra hospital, Deccan college of medical sciences, Hyderabad, Telangana, India. Consecutive intramural and extramural neonates admitted to neonatal intensive care unit with the Rh-negative mother’s blood group and hyperbilirubinemia were enrolled. Neonates born to Rh+ve mothers were excluded. Neonatal gestational age, birth weight, age at admission, duration of phototherapy, duration of hospitalization, neonatal examination and investigations were recorded in a predesigned, pretested performa. Results A total of 90 neonates were born to Rh-negative mothers, of which 70% (63 had the Rh-positive blood group and 30% had the Rh-negative blood group. Of these 63 neonates, 48 (76.2% had hyperbilirubinemia and 43 neonates (68.3% had significant hyperbilirubinemia (total serum bilirubin > 15mg/dL. Among them, 2%, 75% and 23% were born to primi, multi and grandmutli, respectively. Also, 14.5% of the neonates were large for dates (LFD, 75% appropriate for dates (AFD and 10.5% were small for dates (SFD. Premature and SFD neonates had higher incidence of hyperbilirubinemia. Significantly higher incidence of jaundice occurred within 72 hours of life. The mean

  10. System Accuracy Evaluation of the GlucoRx Nexus Voice TD-4280 Blood Glucose Monitoring System

    Directory of Open Access Journals (Sweden)

    Muhammad Khan

    2014-01-01

    Full Text Available Use of blood glucose (BG meters in the self-monitoring of blood glucose (SMBG significantly lowers the risk of diabetic complications. With several BG meters now commercially available, the International Organization for Standardization (ISO ensures that each BG meter conforms to a set degree of accuracy. Although adherence to ISO guidelines is a prerequisite for commercialization in Europe, several BG meters claim to meet the ISO guidelines yet fail to do so on internal validation. We conducted a study to determine whether the accuracy of the GlucoRx Nexus TD-4280 meter, utilized by our department for its cost-effectiveness, complied with ISO guidelines. 105 patients requiring laboratory blood glucose analysis were randomly selected and reference measurements were determined by the UniCel DxC 800 clinical system. Overall the BG meter failed to adhere to the ≥95% accuracy criterion required by both the 15197:2003 (overall accuracy 92.4% and 15197:2013 protocol (overall accuracy 86.7%. Inaccurate meters have an inherent risk of over- and/or underestimating the true BG concentration, thereby risking patients to incorrect therapeutic interventions. Our study demonstrates the importance of internally validating the accuracy of BG meters to ensure that its accuracy is accepted by standardized guidelines.

  11. Effect of volume expansion on systemic hemodynamics and central and arterial blood volume in cirrhosis

    DEFF Research Database (Denmark)

    Møller, S; Bendtsen, F; Henriksen, Jens Henrik Sahl

    1995-01-01

    and in controls. METHODS: Thirty-nine patients with cirrhosis (12 patients with Child-Turcotte class A, 14 with class B, and 13 with class C) and 6 controls were studied. During hepatic vein catheterization, cardiac output, systemic vascular resistance, central and arterial blood volume, noncentral blood volume...

  12. Successful downstream application of the Paxgene Blood RNA system from small blood samples in paediatric patients for quantitative PCR analysis

    Directory of Open Access Journals (Sweden)

    Mankhambo Limangeni A

    2007-09-01

    Full Text Available Abstract Background The challenge of gene expression studies is to reliably quantify levels of transcripts, but this is hindered by a number of factors including sample availability, handling and storage. The PAXgene™ Blood RNA System includes a stabilizing additive in a plastic evacuated tube, but requires 2.5 mL blood, which makes routine implementation impractical for paediatric use. The aim of this study was to modify the PAXgene™ Blood RNA System kit protocol for application to small, sick chidren, without compromising RNA integrity, and subsequently to perform quantitative analysis of ICAM and interleukin-6 gene expression. Aliquots of 0.86 mL PAXgene™ reagent were put into microtubes and 0.3 mL whole blood added to maintain the same recommended proportions as in the PAXgene™ evacuated tube system. RNA quality was assessed using the Agilent BioAnalyser 2100 and an in-house TaqMan™ assay which measures GAPDH transcript integrity by determining 3' to 5' ratios. qPCR analysis was performed on an additional panel of 7 housekeeping genes. Three reference genes (HPRT1, YWHAZ and GAPDH were identified using the GeNORM algorithm, which were subsequently used to normalising target gene expression levels. ICAM-1 and IL-6 gene expression were measured in 87 Malawian children with invasive pneumococcal disease. Results Total RNA yield was between 1,114 and 2,950 ng and the BioAnalyser 2100 demonstrated discernible 18s and 28s bands. The cycle threshold values obtained for the seven housekeeping genes were between 15 and 30 and showed good consistency. Median relative ICAM and IL-6 gene expression were significantly reduced in non-survivors compared to survivors (ICAM: 3.56 vs 4.41, p = 0.04, and IL-6: 2.16 vs 6.73, p = 0.02. Conclusion We have successfully modified the PAXgene™ blood collection system for use in small children and demonstrated preservation of RNA integrity and successful quantitative real-time PCR analysis.

  13. The Contact Activation System (CAS) in cord blood: Measurement of CAS components and comparison with mother's blood. A pilot study.

    Science.gov (United States)

    Uszyński, Mieczysław; Kuczyński, Jarosław; Żekanowska, Ewa; Uszyński, Waldemar

    2015-11-01

    Classical reference data concerning the coagulation system and fibrinolysis in fetuses and newborns date back to the 1990 s. Since that time a number of methodological or other improvements have been implemented, which may cast some doubt on timeliness of the data. The study objective was to measure the levels of Contact Activation System (CAS) components by antigen, i.e. factors XII and XI (FXII, FXI), prekallikrein (PK) and high molecular weight kininogen (HMWK) in cord blood and maternal blood. The study group consisted of 35 healthy parturient women with an uneventful pregnancy and birth. The samples of cord blood and maternal blood were obtained immediately after delivery, before clumping the umbilical cord. The CAS components were measured by immunoenzymatic method (ELISA). The median concentrations of CAS components in cord blood plasma and mother's plasma were as follow: FXII: 1.02 (0.60- 2.58) ng/mg protein vs. 0.94 (0.66-1.86) ng/mg protein (p>0.05); FXI: 2.71(0.03-8.0) ng/mg protein vs. 0.92 (0.03-10.44) ng/mg protein (p>0.05); PK: 168.78 (104.28-261.16) pg/mg protein vs. 113.44 (79.94-146.70) pg/mg protein (p>0.05); HMWK: 2169.45 (1530.64- 2539.83) ng/mg protein vs. 2857.96 (2541.52-3161.04) ng/mg protein (pblood of full-term and healthy fetuses were similar to those observed in mother's blood immediately after delivery. Only high molecular weight kininogen was found to be lower (accounting for 84% of the values noted in mothers). 2. Based on our measurements, we claim that the cited reference data concerning the contact factors in full-term and healthy newborns are underestimated; hence, new reference values need to be determined for each antigen and activity contact factor level. Copyright © 2015. Published by Elsevier Ltd.

  14. Anti-Rh(c), "little c," isoimmunization: the role of rHuEpo in preventing late anemia.

    Science.gov (United States)

    Zuppa, Antonio A; Cardiello, Valentina; Alighieri, Giovanni; Cota, Francesco; D'Antuono, Annamaria; Riccardi, Riccardo; Catenazzi, Piero; Romagnoli, Costantino

    2013-08-01

    The overall prevalence of non-Rh-D isoimmunization seems to lie between 0.15% and 1.1%. Anti-Rh(c) alloimmunization, "little c," occurs in 0.07% of pregnancies and shows a quite broad clinical presentation. Late anemia is a frequent problem occurring in the setting of isoimmunization. It occurs more frequently after intrauterine blood transfusions or exsanguinotransfusion, and it can be thought as a hyporegenerative anemia. The authors describe the use of human recombinant erythropoietin in preventing late anemia in a case of anti-Rh(c) isoimmunization. The use of human recombinant erythropoietin is a valid tool for preventing late-onset anemia due to either anti-Rh-D or non-anti-Rh-D isoimmunization.

  15. GnRH依赖型性早熟遗传调控系统研究进展%Research Progress of the Genetic Regulation System in GnRH-dependent Precocious Puberty

    Institute of Scientific and Technical Information of China (English)

    韩威; 李慧芳; 朱云芬; 宋迟; 徐文娟

    2013-01-01

    性早熟在人类上表现为病理状态,而在家禽生产上则是一个具有重要经济价值的性状.人类性早熟调控机理的研究较为透彻,尤其是近年来全基因关联分析方法的应用,使得研究者对于性成熟启动相关新基因发掘及其调控网络的认识更加深入.论文综述了人类GnRH依赖型性早熟(GDPP)的5个基因调控系统,包括kisspeptin系统、γ-GABA系统、NPY和leptin系统、LIN28B系统和NKB系统的研究进展,为禽类早熟性状研究提供依据.%Precocious puberty performs as pathological condition in humans, but it is an important trait in animal production. The research of human precocious puberty regulation mechanism is more thorough, especially in recent years the genome-wide association study method contributes to find more new genes and deeply understand the genetic networks. This paper summarized the progress of genetic systems in human GnRH-dependent precocious puberty, including kisspeptin system, γ-GABA system, NPY and leptin system, LIN28B system and NKB system. These could supply a basis for the relevant study in animals

  16. Air bubbles and hemolysis of blood samples during transport by pneumatic tube systems.

    Science.gov (United States)

    Mullins, Garrett R; Bruns, David E

    2017-08-10

    Transport of blood samples through pneumatic tube systems (PTSs) generates air bubbles in transported blood samples and, with increasing duration of transport, the appearance of hemolysis. We investigated the role of air-bubble formation in PTS-induced hemolysis. Air was introduced into blood samples for 0, 1, 3 or 5min to form air bubbles. Hemolysis in the blood was assessed by (H)-index, lactate dehydrogenase (LD) and potassium in plasma. In an effort to prevent PTS-induced hemolysis, blood sample tubes were completely filled, to prevent air bubble formation, and compared with partially filled samples after PTS transport. We also compared hemolysis in anticoagulated vs clotted blood subjected to PTS transport. As with transport through PTSs, the duration of air bubble formation in blood by a gentle stream of air predicted the extent of hemolysis as measured by H-index (pblood sample prevented bubble formation and fully protected the blood from PTS-induced hemolysis (pblood developed less foaming during PTS transport and was partially protected from hemolysis vs anticoagulated blood as indicated by lower LD (psample transport. Prevention of air bubble formation in blood samples during PTS transport protects samples from hemolysis. Copyright © 2017. Published by Elsevier B.V.

  17. 基于生物电阻抗的血型检测新方法及系统实现%New Method for Blood Type Detection Based on Bioelectrical lmpedance Analysis Technology and its System lmplementation

    Institute of Scientific and Technical Information of China (English)

    陈里里; 廖强; 胡雪; 张国珍

    2014-01-01

    A new method for blood type detection and a new automated blood typing system are developed. In the system,the combined use of the AD9850 chip and MCU are employed to realize the DDS technique and produce excitation signal. Then an elliptic filter is designed to eliminate the signal interference,and an amplitude stabilized circuit is designed to stabilize the amplitude of the signal output by using an AD8370 chip,which is a low-cost variable gain amplifier. The amplitude and phase measurement chip AD8302 is adopted to extract the impedance information. Based on neural network recognition model,a new method is designed to determine the degree of agglutination in red blood cell suspension. The use of this method combined with the discriminant rule could successfully identify the blood type. Contrastive test is carried out and the new system showed high measurement accuracy and good stability,could better distinguish the different intensity of agglutination in red blood cell,and identified ABO and Rh blood type correctly in normal samples. The self-developed new automated blood typing system is in better agreement with clinical requirements.%提出了基于生物电阻抗测量技术的血型检测新方法并研制了检测系统。该系统采用单片机结合AD9850芯片实现 DDS 技术产生激励信号,设计椭圆滤波器消除信号干扰;运用低成本放大器 AD8370设计稳幅电路,确保信号输出幅度不变;系统采用 AD8302幅相检测芯片实现阻抗信息提取;基于人工神经网络判别红细胞悬浮液凝集强度,并结合判别规则得到血型结果。测试结果表明该系统精度高,稳定性好;临床实验表明,该系统能正确识别红细胞6种不同凝集程度,能100%正确地检测出正常标本的 ABO 和 Rh 血型,可更好地满足临床需求。

  18. One-step design of a stable variant of the malaria invasion protein RH5 for use as a vaccine immunogen

    Science.gov (United States)

    Campeotto, Ivan; Goldenzweig, Adi; Davey, Jack; Barfod, Lea; Marshall, Jennifer M.; Silk, Sarah E.; Wright, Katherine E.; Higgins, Matthew K.; Fleishman, Sarel J.

    2017-01-01

    Many promising vaccine candidates from pathogenic viruses, bacteria, and parasites are unstable and cannot be produced cheaply for clinical use. For instance, Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is essential for erythrocyte invasion, is highly conserved among field isolates, and elicits antibodies that neutralize in vitro and protect in an animal model, making it a leading malaria vaccine candidate. However, functional RH5 is only expressible in eukaryotic systems and exhibits moderate temperature tolerance, limiting its usefulness in hot and low-income countries where malaria prevails. Current approaches to immunogen stabilization involve iterative application of rational or semirational design, random mutagenesis, and biochemical characterization. Typically, each round of optimization yields minor improvement in stability, and multiple rounds are required. In contrast, we developed a one-step design strategy using phylogenetic analysis and Rosetta atomistic calculations to design PfRH5 variants with improved packing and surface polarity. To demonstrate the robustness of this approach, we tested three PfRH5 designs, all of which showed improved stability relative to wild type. The best, bearing 18 mutations relative to PfRH5, expressed in a folded form in bacteria at >1 mg of protein per L of culture, and had 10–15 °C higher thermal tolerance than wild type, while also retaining ligand binding and immunogenic properties indistinguishable from wild type, proving its value as an immunogen for a future generation of vaccines against the malaria blood stage. We envision that this efficient computational stability design methodology will also be used to enhance the biophysical properties of other recalcitrant vaccine candidates from emerging pathogens. PMID:28096331

  19. Blood Pressure Mobile Monitoring for Pregnant Woman Based Android System

    Science.gov (United States)

    Supriyanti, Retno; Erfayanto, Uji; Ramadani, Yogi; Murdyantoro, Eko; Widodo, Haris B.

    2016-01-01

    Currently, at least 18,000 women die every year in Indonesia due to pregnancy or childbirth. It means that every half hour a woman dies due to pregnancy or childbirth. As a result, every year 36,000 children became orphans. The high maternal mortality rate was put Indonesia on top in ASEAN. The main causes of maternal mortality are high-risk pregnancy. Mothers who have diseases like high blood pressure, pre-eclampsia, diabetes, hyperthyroidism, and already over 40 years old and infectious diseases such as rubella, hepatitis and HIV can be factors that lead to high-risk pregnancy. This paper will discuss the development of a blood pressure monitoring device that is suitable for pregnant women. It is based on convenience for pregnant women to get the equipment that is flexible with her presence. Results indicate that the equipment is in use daily support for pregnant women therefore, one of the causes of maternal mortality can be detected earlier.

  20. Reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD negative women

    DEFF Research Database (Denmark)

    Clausen, Frederik Banch; Krog, Grethe Risum; Rieneck, Klaus;

    2005-01-01

    The objective of this study was to establish a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD negative women. This test is needed for future prenatal Rh prophylaxis.......The objective of this study was to establish a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD negative women. This test is needed for future prenatal Rh prophylaxis....

  1. Rh(D)阴性献血者不规则抗体检测的结果分析%Analysis the result of irregular antibody test in Rh (D) negative donors

    Institute of Scientific and Technical Information of China (English)

    李健; 周英; 吕文彬; 陈雪; 迭敏; 龚希

    2012-01-01

    目的 分析Rh(D)阴性献血者不规则抗体检测的结果,探讨在献血者血液Rh(D)阴性确认试验时,进行不规则抗体检测的必要性.方法 用间接抗人球蛋白法进行Rh(D)阴性确认试验及抗体筛选试验、抗体鉴定试验,盐水法进行Rh表现型的检测.结果 1205份Rh(D)阴性献血员标本中,抗体筛选试验阳性的标本有20份,经过抗体鉴定,其中抗-D 10份,抗-M 6份,非特异性抗体2份,抗-Lea 1份,冷自身抗体1份.结论 Rh(D)阴性献血者抗体筛选阳性率高达1.66%,较以往报道的正常人群抗体筛选阳性率高.在临床供血上,其高检出率对临床用血安全有较为重要的意义,对Rh(D)阴性献血者更应该进行不规则抗体检测.%Objective To analyze the result of irregular antibody test in Rh (D) negatie donors, discuss in the necessity of irregular antibody test in Rh (D) negative confirmation experiment among the blood testing with Rh ( D) negative donors. Methods Rh (D) negative confirmation experiment antibody screening and antibody Identification test using indirect antiglobulin test(IAT) , The Rh phenotype were determined by saline method. Results among 120S samples of Rh (D) negative donors, 20 samples antibody screening test is positive. And after antibody Identification test, Among them 10 samples were anti- 0 , 6 samples were anti- M, 2 samples were Nonspecific antibody, 1 sample was anti- Le,l sample was cold antibody. Conclusion The positive rate of Antibody screening in Rh ( D) negative donors is 1. 66% . It is greater than Previous reports of the normal population positive antibody screening. The higher positive rate of Antibody screening has Important clinical significance in blood supply, therefore Rh ( D) negative donors more should do irregular antibody test.

  2. Design of an embedded sensor system for measuring laser scattering on blood cells

    Science.gov (United States)

    Iosifidis, C.; Katsaliaki, K.; Kollensperger, P.; Kiziroglou, M. E.

    2017-05-01

    In this paper, a sensor system architecture for laboratory and in-vivo light scattering studies on blood cells is presented. It aims at correlating Mie scattering to compositional and physiological information of blood cells towards a non-invasive blood-cell counting sensor. An overview of previously reported experimental techniques on light scattering from blood cells is presented. State-of-the-art methods such as differential pulse measurements, vessel pressure optimization identified as promising for enhancing the scattering signal in such measurements. Indicative simulations of Mie scattering by blood cells are presented, illustrating the potential for distinguishing among cells and identifying size distribution. A prototype sensor system based on a 640-660 nm laser light source and a photo diode array is implemented and programmed to obtain mean amplitude and scattering angle measurements.

  3. Does Pneumatic Tube System Transport Contribute to Hemolysis in ED Blood Samples?

    National Research Council Canada - National Science Library

    Phelan, Michael P; Reineks, Edmunds Z; Hustey, Fredric M; Berriochoa, Jacob P; Podolsky, Seth R; Meldon, Stephen; Schold, Jesse D; Chamberlin, Janelle; Procop, Gary W

    2016-01-01

    Our goal was to determine if the hemolysis among blood samples obtained in an emergency department and then sent to the laboratory in a pneumatic tube system was different from those in samples that were hand-carried...

  4. Switching substrate specificity of AMT/MEP/ Rh proteins

    Science.gov (United States)

    Neuhäuser, Benjamin; Dynowski, Marek; Ludewig, Uwe

    2014-01-01

    In organisms from all kingdoms of life, ammonia and its conjugated ion ammonium are transported across membranes by proteins of the AMT/Rh family. Efficient and successful growth often depends on sufficient ammonium nutrition. The proteins mediating this transport, the so called Ammonium Transporter (AMT) or Rhesus like (Rh) proteins, share a very similar trimeric overall structure and a high sequence similarity even throughout the kingdoms. Even though structural components of the transport mechanism, like an external substrate recruitment site, an essential twin histidine pore motif, a phenylalanine gate and the hydrophobic pore are strongly conserved and have been analyzed in detail by molecular dynamic simulations and mutational studies, the substrate(s), which pass the central pores of the AMT/Rh subunits, NH4+, NH3 + H+, NH4+ + H+ or NH3, are still a matter of debate for most proteins, including the best characterized AmtB protein from Escherichia coli. The lack of a robust expression system for functional analysis has hampered proof of structural and mutational studies, although the NH3 transport function for Rh-like proteins is rarely disputed. In plant transporters belonging to the subfamily AMT1, transport is associated with electrical currents, while some plant transporters, notably of the AMT2 type, were suggested to transport NH3 across the membrane, without associated ionic currents. Here we summarize data in favor of each substrate for the distinct AMT/Rh classes, discuss mutants and how they differ in structure and functionality. A common mechanism with deprotonation and subsequent NH3 transport through the central subunit pore is suggested. PMID:25483282

  5. The Study of ABO Groups and Rh Factor in Active and Non-active Carriers of Hepatitis B Virus

    Directory of Open Access Journals (Sweden)

    Haydeh Alaoddolehei et al.

    2007-01-01

    there is no significant difference between blood group (p=0.145 or Rh factor (p=0.329 and active or non-active form. This finding was in agreement with others, for example Maria Rios showed that there is a relationship between parvovirus B19 and P antigen and also between Hemophilos influenza and Anwy antigen (2. Another study proved that the adherence of Norwalk virus is more deficiently in low concentration of H1 epitope (6. However, to the best of author's knowledge, there is low amount of information about relationship between HBV and blood groups. Thus, it seems more studies should be accomplished in this field. Meanwhile, it is necessary to examine the patients * Correspondence: Haydeh Alaoddolehei MS, Paramedical Faculty, Babol University of Medical Sciences, Ganjafrooz Avenue, Babol, Iran. Tel: +98 111 2234274 Fax: +98 111 2234367 E-mail: alaee_ha@yahoo.com Received: 13 Feb 2006 Revised: 19 Sep 2006 Accepted: 22 Sep 2006 Hep Mon 2007; 7 (1: 43-44 for secretory forms and other blood group systems in active carriers. References 1. Gerald L. Mandell. Douglas. Principles and practice of infectious disease, fifth ed, Churchill, volume 1, 2000, 39. 2. Rios M, Bianco C. The role of blood group antigens in infectious disease. Hematology 2000; 37: 177-86. 3. Tilzey AD, et al. Zuckermonted, Viral Hepatitis fever diseases, Alen. R, Liss, 1988, 1047. 4. Gerald L. Mandell, Principles and practice of infectious disease, fifth ed, Churchill, volume 3, 2000, 1285-6, 1299- 300. 5. Golafshan J, Ghahremani MJ, Sharifzadeh S, The principles and methods of blood banking, Fourth ed, Shiraz Medical University, 2001, 91. 6. Marionneau S, Airaud F. Influence of the combind ABO, FUT2 and FUT3 polymorphism on susceptibility to nerwalk virus attachment, J infect Dis, 2005 sep 15; 192 (6: 1071-7, Epub 2005 Aug. http://www/nlm.nih.gov/- researchers/med/pub.

  6. Effects of Replenishing Qi, Promoting Blood Circulation and Resolving Phlegm on Vascular Endothelial Function and Blood Coagulation System in Senile Patients with Hyperlipemia

    Institute of Scientific and Technical Information of China (English)

    Yang Huimin; Han Libei; Sheng Tong; He Qiong; Liang Jinpu

    2006-01-01

    Objective: To observe the curative effect of the method of replenishing qi, promoting blood circulation and resolving phlegm on senile hyperlipemia and its effects on vascular endothelial function and blood coagulation system. Method: 96 patients with senile hyperlipemia were randomly divided into a treatment group and a of blood lipid, vascular endothelial function, blood coagulation system and safety. Results: After treatment,the treatment group was obviously superior to the control group (P<0.05) in reducing plasma total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) as well as in the ratio of thromboxane B2 (TXB2) to 6-keto-prostaglandin F1α (6-keto-PGF1α), D-dimer (D-D) and fibrinogen (FIB). Conclusion: Danshen Jueming Granules have the effect of regulating metabolism of blood lipid, and improving vascular endothelial function and blood coagulation system in senile patients with hyperlipemia.

  7. Towards tailoring the magnetocaloric response in FeRh-based ternary compounds

    Energy Technology Data Exchange (ETDEWEB)

    Barua, Radhika, E-mail: barua.r@husky.neu.edu; Jiménez-Villacorta, Félix; Lewis, L. H. [Department of Chemical Engineering, Northeastern University, Boston, Massachusetts 02115 (United States)

    2014-05-07

    In this work, we demonstrate that the magnetocaloric response of FeRh-based compounds may be tailored for potential magnetic refrigeration applications by chemical modification of the FeRh lattice. Alloys of composition Fe(Rh{sub 1−x}A{sub x}) or (Fe{sub 1−x}B{sub x})Rh (A = Cu, Pd; B = Ni; 0 < x < 0.06) were synthesized via arc-melting and subsequent annealing in vacuum at 1000 °C for 48 h. The magnetocaloric properties of the FeRh-based systems were determined using isothermal M(H) curves measured in the vicinity of the magnetostructural temperature (T{sub t}). It is found that the FeRh working temperature range (δT{sub FWHM}) may be chemically tuned over a wide temperature range, 100 K ≤ T ≤ 400 K. While elemental substitution consistently decreases the magnetic entropy change (ΔS{sub mag}) of the FeRh-based ternary alloys from that of the parent FeRh compound (ΔS{sub mag},{sub FeRh} ∼ 17 J/kg K; ΔS{sub mag,FeRh-ternary =} 7–14 J/kg K at H{sub app} = 2 T), the net refrigeration capacity (RC), defined as the amount of heat that can be transferred during one magnetic refrigeration cycle, of the modified systems is significantly higher (RC{sub FeRh} ∼ 150 J/kg; RC{sub FeRh-ternary =} 170–210 J/kg at H{sub app} = 2 T). These results are attributed to stoichiometry-induced changes in the FeRh electronic band structure and beneficial broadening of the magnetostructural transition due to local chemical disorder.

  8. Distribution of blood groups in blood donors in the blood bank of Jagdalpur, Bastar district, Chhattisgarh

    Directory of Open Access Journals (Sweden)

    Sachin A Badge

    2017-01-01

    Full Text Available Aims and Objectives: The incidence of ABO and rhesus (Rh groups varies markedly in different races, ethnic groups, and socioeconomic groups in different parts of the world. The frequencies of ABO and Rh blood groups vary from one population to another and time to time in the same region. The present study was carried out to find the distribution of blood group in rural and tribal populations of Bastar district of Chhattisgarh. Materials and Methods: The present retrospective study was carried out at late Shri Baliram Kashyap Memorial Government Medical College and Maharani Hospital blood bank, Jagdalpur, Bastar district, Chhattisgarh, India, during the 2-year period from January 2014 to December 2015. The blood collections were taken from the voluntary donors at outdoor blood donation camp and in-house blood bank as well as from replacement donors at blood bank. Totally 12,852 donors were considered medically fit and accepted for blood donation during the study period. Results: Out of the total 12,852 donors, most of the donors, i.e., 3996 (31.09% were with blood Group O followed by B (30.44%, A (24.95%, and AB (13.52%. Out of the 12,852 blood donors, majority, i.e., 12,779 (99.43% were male and 73 (0.57% were female. Maximum blood donors, i.e., 12,777 (99.42% were Rh positive while only 75 (0.58% were Rh negative. Conclusion: The knowledge of distribution of ABO and Rh blood groups at local and regional levels is helpful in effective management of blood banks and safe blood transfusion services.

  9. Understanding and controlling the structure and segregation behaviour of AuRh nanocatalysts

    Science.gov (United States)

    Piccolo, Laurent; Li, Z. Y.; Demiroglu, Ilker; Moyon, Florian; Konuspayeva, Zere; Berhault, Gilles; Afanasiev, Pavel; Lefebvre, Williams; Yuan, Jun; Johnston, Roy L.

    2016-01-01

    Heterogeneous catalysis, which is widely used in the chemical industry, makes a great use of supported late-transition-metal nanoparticles, and bimetallic catalysts often show superior catalytic performances as compared to their single metal counterparts. In order to optimize catalyst efficiency and discover new active combinations, an atomic-level understanding and control of the catalyst structure is desirable. In this work, the structure of catalytically active AuRh bimetallic nanoparticles prepared by colloidal methods and immobilized on rutile titania nanorods was investigated using aberration-corrected scanning transmission electron microscopy. Depending on the applied post-treatment, different types of segregation behaviours were evidenced, ranging from Rh core – Au shell to Janus via Rh ball – Au cup configuration. The stability of these structures was predicted by performing density-functional-theory calculations on unsupported and titania-supported Au-Rh clusters; it can be rationalized from the lower surface and cohesion energies of Au with respect to Rh, and the preferential binding of Rh with the titania support. The bulk-immiscible AuRh/TiO2 system can serve as a model to understand similar supported nanoalloy systems and their synergistic behaviour in catalysis. PMID:27739480

  10. Social status, breeding state, and GnRH soma size in convict cichlids (Cryptoheros nigrofasciatus).

    Science.gov (United States)

    Chee, San-San Amy; Espinoza, Walter A S; Iwaniuk, Andrew N; Pakan, Janelle M P; Gutiérrez-Ibáñez, Cristian; Wylie, Douglas R; Hurd, Peter L

    2013-01-15

    Gonadotropin-releasing hormone (GnRH) expressing neurons in the preoptic area (POA) of the hypothalamus plays a key role in regulating reproductive function through the control of gonadotropin release. Several studies have illustrated the importance of the social environment in modulating the size of GnRH expressing neurons. In the African cichlid fish Astatotilapia burtoni, the size of the soma of GnRH expressing neurons in the POA varies with social status in males, and with breeding state in females. Territorial males have larger GnRH+ cells than non-territorial males, while brooder females have smaller GnRH+ cells than control females. The lek-like breeding system of A. burtoni is, however, only one type of social system within the diverse assemblage of cichlids. To gain a better understanding of GnRH neuronal plasticity in response to the changes in the social environment, we tested whether similar effects occur in the monogamous New World cichlid, the convict cichlid (Cryptoheros nigrofasciatus), a model species for the study of social behaviour. Our results indicate that, indeed GnRH expressing neuron soma size, and not cell number, varies with both male territorial status, and manipulations of female breeding state in this monogamous, biparental, New World cichlid.

  11. Understanding and controlling the structure and segregation behaviour of AuRh nanocatalysts

    Science.gov (United States)

    Piccolo, Laurent; Li, Z. Y.; Demiroglu, Ilker; Moyon, Florian; Konuspayeva, Zere; Berhault, Gilles; Afanasiev, Pavel; Lefebvre, Williams; Yuan, Jun; Johnston, Roy L.

    2016-10-01

    Heterogeneous catalysis, which is widely used in the chemical industry, makes a great use of supported late-transition-metal nanoparticles, and bimetallic catalysts often show superior catalytic performances as compared to their single metal counterparts. In order to optimize catalyst efficiency and discover new active combinations, an atomic-level understanding and control of the catalyst structure is desirable. In this work, the structure of catalytically active AuRh bimetallic nanoparticles prepared by colloidal methods and immobilized on rutile titania nanorods was investigated using aberration-corrected scanning transmission electron microscopy. Depending on the applied post-treatment, different types of segregation behaviours were evidenced, ranging from Rh core - Au shell to Janus via Rh ball - Au cup configuration. The stability of these structures was predicted by performing density-functional-theory calculations on unsupported and titania-supported Au-Rh clusters; it can be rationalized from the lower surface and cohesion energies of Au with respect to Rh, and the preferential binding of Rh with the titania support. The bulk-immiscible AuRh/TiO2 system can serve as a model to understand similar supported nanoalloy systems and their synergistic behaviour in catalysis.

  12. Magnetic and Transport Studies in LaxGd1-xRh3B2 and CexGd1-xRh3B2

    Science.gov (United States)

    Vaidya, Ulhas; Dhar, S. K.; Thamizhavel, A.; Ramakrishnan, S.; Grover, A. K.

    2010-03-01

    The nonmagnetic rare earth ternary boride LaRh3B2, a hexagonal system of space group P6/mmm with CeCo3B2 type structure, is known to superconduct below Tc(0)˜ 2.7 K. When doped with Gd the Tc(0) reduces and no superconductivity is observed above 1.2 K with more than 15% (atomic) doping. The alloys, La0.8Gd0.2Rh3B2 and La0.6Gd0.4Rh3B2 show a jump at ˜ 70 K and rapid rise in magnetization below 10 K. However, no peaks are observed in at these temperatures in AC susceptibility which would have indicated magnetic ordering of the system. It is worth noting that GdRh3B2 orders ferromagnetically at ˜ 93 K. We shall present results of our investigations, above 2 K, in magnetic and transport properties of polycrystalline specimens LaxGd1-xRh3B2 (0 CeRh3B2 is a ferromagnet with Tc ˜ 115 K. The results of Gd doping in this reveal the survival of antiferromagnetic coupling between Ce and Gd spins.

  13. Repeated action of a constant magnetic field on the blood coagulation system in artificially produced anemia

    Science.gov (United States)

    Zabrodina, L. V.

    1974-01-01

    Changes are discussed in the coagulatory system of the blood in rabbits under the influence of a constant magnetic field of an intensity of 2500 oersteds against the background of artificially induced anemia. Reversibility of the changes produced and the presence of the adaptational effect are noted. Taking all this into consideration, the changes involving the coagulatory system of the blood which arise under the influence of a constant magnetic field may be considered to have a nerve-reflex nature.

  14. Development of blood vessel search system using near-infrared light for laparoscopic surgery

    Science.gov (United States)

    Narita, K.; Nakamachi, E.; Morita, Y.; Hagiwara, A.

    2013-09-01

    Our objective of this study is to develop a miniature and high accuracy automatic 3D blood vessel searching system, which will be introduced in the laparoscopic operation with the minimally invasive surgery. Now, the conventional optical system used in the laparoscopic surgery has many difficulties of blood vessel imaging and detection, because the peripheral bio-tissue located around the blood vessel disturbs the light wave propagation, disperses and refracts. Consequently, only unclear image is obtained. We develop a new blood vessel detecting system by using Near-Infrared (NIR) light, two CMOS camera modules and a comprehensive image processing technique, which is implemented in the laparoscope pipe with 25mm in diameter. We adopt the stereo method for the searching system to determine 3D blood vessel location. The blood vessel visualization system adopts hemoglobin's absorption characteristics of the NIR light, which has high permeability for the bio-tissue and absorbency for the hemoglobin. A sharpening process is employed to improve the image quality of original ones, through the LoG filter and the un-sharp-mask processing. 2D location of the blood vessel is calculated from luminance distribution of the image and its depth is calculated by the stereo method. A validity of our blood vessel visualization and 3D detecting system was examined through the comparison with the imaging and detecting the results of organization phantoms, which embedded at known depths under the surface. Experimental results of depth obtained by our detecting system showed good agreements with the given depths, and the availability of this system is confirmed.

  15. A RhIII-N-heterocyclic carbene complex from metal-metal singly bonded [RhII−RhII] precursor

    Indian Academy of Sciences (India)

    Arup Sinha; Abir Sarbajna; Shrabani dinda; Jitendra K Bera

    2011-11-01

    Metal-metal singly bonded [Rh2(CO)4(acac)2][OTf]2 (1) has been synthesized and characterized by spectroscopic and analytical techniques. A density functional theory (DFT) optimized structure has been computed for the unbridged centro-symmetric structure. Reaction of 1 with PIN.HBr results in the [Rh(PIN)2(H2O)Br][OTf]2 (2) in high yield. The reaction involves metal-oxidation from RhII to RhIII accompanied by the metal-metal bond cleavage. The X-ray structure of 2 has been determined which reveals the incorporation of two N-heterocyclic carbene (NHC) ligands to each rhodium. This work demonstrates the general utility of the metal-metal bonded compounds for the easy synthesis of metal-NHC compounds.

  16. Blood Groups in the Kashmir Valley

    Directory of Open Access Journals (Sweden)

    Rafiq A.Calcutti, Mohammed Khali Lone, Showket Ahmed,Bashir A.Shah, Neelofer Jan.

    2003-07-01

    Full Text Available Blood groups are genetically determined and exJ1ibit polymorphism, where different populationgroups have significant difference in the frequency of each blood group. This study wasconducted to determine the frequency of ABO and Rhesus D blood groups among the blooddO:lors. A total number of 1306 blood donors attended the donor centre at SKIMS MedicalCollege Hospital for blood donation in the year 2001-02. After each donation blood sampleswere collected in separate pilot hlbes for the estimation of ABO and Rhesus D blood groups.The frequency of O. A, Band AB, Rhesus D positive and Rhesus D negative were calculatedseparately. The highest li"equency among the ABO blood groups was ofB (39.43% and the lowestwas of AB (8.11 %. Among the Rhesus D phenotypes. majority (93.33% were RhesusD positive. where as only 6.67% were Rhesus D negative. The prevalence of ABO/Rhesus D wascalculated and the highest frequency was o1'B Rh-D positive (37.44% followed by a Rh-D positive(28.9-+%. A Rh-D positive (19.21 %, AB Rh-D positive (7.73%, a Rh-D negative (2.90%,B Rh-D negati'c (1.99%, A Rh-D negative (1.37% and AB Rh-D negative (0.38%. Thisstudy showed that most common group was B followed by a & A and 93.33% were positive forRh-D phenotype.

  17. Effect of epinephrine, norepinephrine and(or) GnRH on serum LH in prepuberal beef heifers.

    Science.gov (United States)

    Hardin, D R; Randel, R D

    1983-09-01

    Forty prepuberal Simmental X Brahman-Hereford heifers were utilized to determine the effects of epinephrine (E), norepinephrine (NE), gonadotropin releasing hormone (GnRH) or combinations of GnRH + E and GnRH + NE on serum luteinizing hormone (LH) concentrations. Animals were assigned randomly to one of five treatments with four replicates/treatment. Treatments consisted of I) 100 micrograms GnRH at time 0 (n = 8); II) 50 mg NE at time -15 and 0 (n = 8); III) 50 mg E at time -15 and 0 (n = 8); IV) 100 micrograms GnRH at time 0, plus 50 mg NE at time -15 and 0 (n = 8) and V) 100 micrograms GnRH at time 0, plus 50 mg E at time -15 and 0 (n = 8). All treatment compounds were administered im in 2 ml physiological saline and blood samples were collected via tail vessel puncture at -30, -15, 0, 15, 30, 45, 60, 90, 120, 180, 240, 300 and 360 min from GnRH injection. Treatment with NE or E alone had no effect (P greater than .10) on serum LH during the sampling period. The initial LH release to GnRH was altered (P less than .05) by concomitant treatment with NE (treatment IV) or E (treatment V). Magnitude of the LH release was reduced (P less than .01) by treatment V. Area under the LH surge was reduced (P less than .05) by treatment IV (NE) and V (E).

  18. Evaluation of the antimicrobial removal device when used with the BACTEC blood culture system

    Energy Technology Data Exchange (ETDEWEB)

    Strand, C.L.

    1982-12-01

    A study to determine the value of the Antimicrobial Removal Device (ARD) used in conjunction with radiometric detection of bacteremia using three media was conducted. During a 12-month period, 622 duplicate ARD/BACTEC blood-culture sets were collected. There were 88 positive cultures that yielded 68 pathogenic isolates and 28 probable contaminant isolates. When all patients were considered, 31 pathogenic isolates were detected by both systems, 25 pathogenic isolates were detected faster or only by the BACTEC system, and 12 pathogenic isolates were detected faster or only when the ARD was employed. This difference is statistically significant (P less than 0.05). Thus, the standard BACTEC blood-culture system using three different media was superior to the same BACTEC system using ARD-processed blood specimens. When only patients receiving antimicrobial therapy were considered, there were more pathogenic isolates detected from unprocessed blood than from blood processed in the ARD; however, this difference was not statistically significant. In conclusion, there appears to be no advantage to using the ARD system in conjunction with the three-bottle BACTEC blood-culture system.

  19. Continuous blood pressure recordings simultaneously with functional brain imaging: studies of the glymphatic system

    Science.gov (United States)

    Zienkiewicz, Aleksandra; Huotari, Niko; Raitamaa, Lauri; Raatikainen, Ville; Ferdinando, Hany; Vihriälä, Erkki; Korhonen, Vesa; Myllylä, Teemu; Kiviniemi, Vesa

    2017-03-01

    The lymph system is responsible for cleaning the tissues of metabolic waste products, soluble proteins and other harmful fluids etc. Lymph flow in the body is driven by body movements and muscle contractions. Moreover, it is indirectly dependent on the cardiovascular system, where the heart beat and blood pressure maintain force of pressure in lymphatic channels. Over the last few years, studies revealed that the brain contains the so-called glymphatic system, which is the counterpart of the systemic lymphatic system in the brain. Similarly, the flow in the glymphatic system is assumed to be mostly driven by physiological pulsations such as cardiovascular pulses. Thus, continuous measurement of blood pressure and heart function simultaneously with functional brain imaging is of great interest, particularly in studies of the glymphatic system. We present our MRI compatible optics based sensing system for continuous blood pressure measurement and show our current results on the effects of blood pressure variations on cerebral brain dynamics, with a focus on the glymphatic system. Blood pressure was measured simultaneously with near-infrared spectroscopy (NIRS) combined with an ultrafast functional brain imaging (fMRI) sequence magnetic resonance encephalography (MREG, 3D brain 10 Hz sampling rate).

  20. Mathematical modeling of the GnRH pulse and surge generator

    CERN Document Server

    Clement, Frederique

    2007-01-01

    We propose a mathematical model allowing for the alternating pulse and surge pattern of GnRH (Gonadotropin Releasing Hormone) secretion. The model is based on the coupling between two systems running on different time scales. The faster system corresponds to the average activity of GnRH neurons, while the slower one corresponds to the average activity of regulatory neurons. The analysis of the slow/fast dynamics exhibited within and between both systems allows to explain the different patterns (slow oscillations, fast oscillations and periodical surge) of GnRH secretion. Specifications on the model parameter values are derived from physiological knowledge in terms of amplitude, frequency and plateau length of oscillations. The behavior of the model is finally illustrated by numerical simulations reproducing natural ovarian cycles and either direct or indirect actions of ovarian steroids on GnRH secretion.

  1. ABO and Rh (D group distribution and gene frequency; the first multicentric study in India

    Directory of Open Access Journals (Sweden)

    Amit Agrawal

    2014-01-01

    Full Text Available Background and Objectives: The study was undertaken with the objective to provide data on the ABO and Rh(D blood group distribution and gene frequency across India. Materials and Methods: A total of 10,000 healthy blood donors donating in blood banks situated in five different geographical regions of the country (North, South, East and Center were included in the study. ABO and Rh (D grouping was performed on all these samples. Data on the frequency of ABO and Rh(D blood groups was reported in simple numbers and percentages. Results: The study showed that O was the most common blood group (37.12% in the country closely followed by B at 32.26%, followed by A at 22.88% while AB was the least prevalent group at 7.74%. 94.61% of the donor population was Rh positive and the rest were Rh negative. Regional variations were observed in the distribution. Using the maximum likelihood method, the frequencies of the I A , I B and I O alleles were calculated and tested according to the Hardy Weinberg law of Equilibrium. The calculated gene frequencies are 0.1653 for I A (p, 0.2254 for I B (q and 0.6093 for I O (r. In Indian Population, O (r records the highest value followed by B (q and A (p; O > B > A. Conclusion: The study provides information about the relative distribution of various alleles in the Indian population both on a pan-India basis as well as region-wise. This vital information may be helpful in planning for future health challenges, particularly planning with regards to blood transfusion services.

  2. ABO and Rh (D) group distribution and gene frequency; the first multicentric study in India

    Science.gov (United States)

    Agrawal, Amit; Tiwari, Aseem Kumar; Mehta, Nidhi; Bhattacharya, Prasun; Wankhede, Ravi; Tulsiani, Sunita; Kamath, Susheela

    2014-01-01

    Background and Objectives: The study was undertaken with the objective to provide data on the ABO and Rh(D) blood group distribution and gene frequency across India. Materials and Methods: A total of 10,000 healthy blood donors donating in blood banks situated in five different geographical regions of the country (North, South, East and Center) were included in the study. ABO and Rh (D) grouping was performed on all these samples. Data on the frequency of ABO and Rh(D) blood groups was reported in simple numbers and percentages. Results: The study showed that O was the most common blood group (37.12%) in the country closely followed by B at 32.26%, followed by A at 22.88% while AB was the least prevalent group at 7.74%. 94.61% of the donor population was Rh positive and the rest were Rh negative. Regional variations were observed in the distribution. Using the maximum likelihood method, the frequencies of the IA, IB and IO alleles were calculated and tested according to the Hardy Weinberg law of Equilibrium. The calculated gene frequencies are 0.1653 for IA (p), 0.2254 for IB (q) and 0.6093 for IO (r). In Indian Population, O (r) records the highest value followed by B (q) and A (p); O > B > A. Conclusion: The study provides information about the relative distribution of various alleles in the Indian population both on a pan-India basis as well as region-wise. This vital information may be helpful in planning for future health challenges, particularly planning with regards to blood transfusion services. PMID:25161353

  3. Rh-flash acquisition card

    CERN Document Server

    Bourrion, O

    2003-01-01

    The rh-flash card main purpose is to convert and store the image of the analog data present at input into an output buffer, namely in a given timing window besides a stop signal (like a digital oscilloscope). It is conceived in VME format 1U wide with an additional connector. Novelty of this card is its ability to sample at a high frequency, due to flash coders, and this at a high repetition rate. To do that the card allows the storage of the data considered 'useful' and that is done by storing only the data exceeding a certain threshold. This can be useful for instance for viewing peaks in a spectrum, and obtaining their relative location. The goal is to stock and process the data sampled before and after the arrival of a stop signal (what entails a storage depth). A threshold is defined and any peak exceeding its level will really be stored in the output buffer which is readable through the VME bus. The peak values will be stored as well as m preceding and n subsequent values (both programmable). Obviously,...

  4. (Bifunctional chelates of Rh-105, Au-199, and other metallic radionuclides as potential radiotherapeutic agents)

    Energy Technology Data Exchange (ETDEWEB)

    1991-01-01

    Progress during this period is reported under the following headings: Diethylenetriamine based and related bifunctional chelating agents and their complexation with Rh-105, Au-198, Pd-109, cu-67, In-111, and Co-57; studies of Pd-109, Rh-105 and Tc-99m with bifunctional chelates based on phenylenediamine; establishment of an appropriate protein assay method for conjugated proteins; studies of new bifunctional Bi, Tri and tetradentate amine oxime ligands with Rh-105; IgG and antibody B72.3 conjugation studies by HPLC Techniques with bifunctional metal chelates; and progress on ligand systems for Au(III).

  5. [Bifunctional chelates of Rh-105, Au-199, and other metallic radionuclides as potential radiotherapeutic agents

    Energy Technology Data Exchange (ETDEWEB)

    1991-12-31

    Progress during this period is reported under the following headings: Diethylenetriamine based and related bifunctional chelating agents and their complexation with Rh-105, Au-198, Pd-109, cu-67, In-111, and Co-57; studies of Pd-109, Rh-105 and Tc-99m with bifunctional chelates based on phenylenediamine; establishment of an appropriate protein assay method for conjugated proteins; studies of new bifunctional Bi, Tri and tetradentate amine oxime ligands with Rh-105; IgG and antibody B72.3 conjugation studies by HPLC Techniques with bifunctional metal chelates; and progress on ligand systems for Au(III).

  6. Ammonia excretion in rainbow trout (Oncorhynchus mykiss): evidence for Rh glycoprotein and H+-ATPase involvement.

    Science.gov (United States)

    Nawata, C Michele; Hung, Carrie C Y; Tsui, Tommy K N; Wilson, Jonathan M; Wright, Patricia A; Wood, Chris M

    2007-11-14

    Branchial ammonia transport in freshwater teleosts is not well understood. Most studies conclude that NH(3) diffuses out of the gill and becomes protonated to NH(4)(+) in an acidified gill boundary layer. Rhesus (Rh) proteins are new members of the ammonia transporter superfamily and rainbow trout possess genes encoding for Rh30-like1 and Rhcg2. We identified seven additional full-length trout Rh cDNA sequences: one Rhag and two each of Rhbg, Rhcg1, and Rh30-like. The mRNA expression of Rhbg, Rhcg1, and Rhcg2 was examined in trout tissues (blood, brain, eye, gill, heart, intestine, kidney, liver, muscle, skin, spleen) exposed to high external ammonia (HEA; 1.5 mmol/l NH(4)HCO(3), pH 7.95, 15 degrees C). Rhbg was expressed in all tissues, Rhcg1 was expressed in brain, gill, liver, and skin, and Rhcg2 was expressed in gill and skin. Brain Rhbg and Rhcg1 were downregulated, blood Rh30-like and Rhag were downregulated, and skin Rhbg and Rhcg2 were upregulated with HEA. After an initial uptake of ammonia into the fish during HEA, excretion was reestablished, coinciding with upregulations of gill Rh mRNA in the pavement cell fraction: Rhcg2 at 12 and 48 h, and Rhbg at 48 h. NHE2 expression remained unchanged, but upregulated H(+)-ATPase (V-type, B-subunit) and downregulated carbonic anhydrase (CA2) expression and activity were noted in the gill and again expression changes occurred in pavement cells, and not in mitochondria-rich cells. Together, these results indicate Rh glycoprotein involvement in ammonia transport and excretion in the rainbow trout while underscoring the significance of gill boundary layer acidification by H(+)-ATPase.

  7. The Rh oxide ultrathin film on Rh(100): an x-ray photoelectron diffraction study.

    Science.gov (United States)

    Zhan, Rong Rong; Vesselli, Erik; Baraldi, Alessandro; Lizzit, Silvano; Comelli, Giovanni

    2010-12-07

    The surface and interface structure of the RhO(2) ultrathin film grown on Rh(100) is investigated by means of x-ray photoelectron diffraction. Experimental and simulated one- and two-dimensional angular distribution intensities of the O1s and Rh3d(5/2) chemically shifted core levels are quantitatively analyzed. The previously proposed O-Rh-O trilayer model is independently confirmed. A rippled buckling of the metal surface is observed at the oxide-metal interface, with a mean interfacial Rh-O distance which is 0.2 Å larger with respect to previous findings. The link between the local atomic rearrangement and the overall geometric and electronic properties of the oxide is discussed on the basis of a thorough comparison with the corresponding RhO(2) rutile structure.

  8. A well-defined (POCOP)Rh catalyst for the coupling of aryl halides with thiols.

    Science.gov (United States)

    Timpa, Samuel D; Pell, Christopher J; Ozerov, Oleg V

    2014-10-22

    This article describes a well-defined pincer-Rh catalyst for C-S cross-coupling reactions. (POCOP)Rh(H)(Cl) serves as an active precatalyst for the coupling of aryl chlorides and bromides with aryl and alkyl thiols under reasonable conditions (3% mol cat., 110 °C, 2-24 h, >90% yield). For select substrates, >90% yields were obtained with catalyst loading as low as 0.1%. Key mechanistic intermediates have been isolated and fully characterized, including (POCOP)Rh(Ph)(SPh) (6a) and (POCOP)Rh(SPh2) (6b). The aryl/bis(phosphinite) (POCOP)Rh system has been shown to favor aryl thiolate reductive elimination at elevated temperatures and in some cases at room temperature, compared with the analogous diarylamido/bis(phosphine) (PNP)Rh pincer system. Concerted reductive elimination has been studied with 6a directly and in the presence of aryl bromide and aryl chloride traps. This investigation demonstrates a clear rate dependence on aryl chloride concentration during catalysis, a dependence that is absent when using aryl bromides. The rate of catalysis is dramatically reduced or brought to zero for ortho-tolyl halides, which can be traced to slower C-S coupling and slower carbon-halogen oxidative addition for ortho-substituted aryls. The influence of the sterics in the thiol component is less straightforward. The S-H oxidative addition product (POCOP)Rh(H)(SPh) (16) has been fully characterized and its reactivity has been examined, resulting in the isolation of the sodium-thiolate adduct (POCOP)Rh(NaSPh) (19). The solid-state structure of 19 shows Na interactions not only with sulfur, but also with a neighboring Rh and the chelating aryl carbon of the pincer framework. The reactivity of 16 and 19 indicates that these potential side products should not hinder catalysis.

  9. System Design and Development of a Robotic Device for Automated Venipuncture and Diagnostic Blood Cell Analysis.

    Science.gov (United States)

    Balter, Max L; Chen, Alvin I; Fromholtz, Alex; Gorshkov, Alex; Maguire, Tim J; Yarmush, Martin L

    2016-10-01

    Diagnostic blood testing is the most prevalent medical procedure performed in the world and forms the cornerstone of modern health care delivery. Yet blood tests are still predominantly carried out in centralized labs using large-volume samples acquired by manual venipuncture, and no end-to-end solution from blood draw to sample analysis exists today. Our group is developing a platform device that merges robotic phlebotomy with automated diagnostics to rapidly deliver patient information at the site of the blood draw. The system couples an image-guided venipuncture robot, designed to address the challenges of routine venous access, with a centrifuge-based blood analyzer to obtain quantitative measurements of hematology. In this paper, we first present the system design and architecture of the integrated device. We then perform a series of in vitro experiments to evaluate the cannulation accuracy of the system on blood vessel phantoms. Next, we assess the effects of vessel diameter, needle gauge, flow rate, and viscosity on the rate of sample collection. Finally, we demonstrate proof-of-concept of a white cell assay on the blood analyzer using in vitro human samples spiked with fluorescently labeled microbeads.

  10. Noninvasive cerebral blood oxygenation monitoring: clinical test of multiwavelength optoacoustic system

    Science.gov (United States)

    Petrov, Y. Y.; Prough, D. S.; Petrova, I.; Patrikeev, I. A.; Cicenaite, I.; Esenaliev, R. O.

    2007-02-01

    Continuous monitoring of cerebral blood oxygenation is critically important for treatment of patients with life-threatening conditions like severe brain injury or during cardiac surgery. We designed and built a novel multiwavelength optoacoustic system for noninvasive, continuous, and accurate monitoring of cerebral blood oxygenation. We use an Optical Parametric Oscillator as a light source. We successfully tested the system in vitro as well as in vivo in large animals (sheep) through thick tissues overlying blood vessels which drain venous blood out of the brain (e.g., superior sagittal sinus or jugular vein). Here we present the results of clinical tests of the system for continuous noninvasive cerebral blood oxygenation monitoring in the internal jugular vein of healthy volunteers. We applied our custom-built optoacoustic probe (which incorporated a wide-band acoustic transducer and an optical fiber) to the neck area overlying the internal jugular vein. We performed measurements with volunteers at 18 wavelengths in the near-infrared spectral range. Despite a thick layer of overlying connective tissue and low energy used in the experiments, we recorded signals with high signal-to-noise ratios for all volunteers. We found that the temporal (independent of signal amplitude) parameters of recorded profiles for different levels of blood oxygenation correlated well with the spectrum of effective attenuation coefficients of blood.

  11. Genomic analyses of RH alleles to improve transfusion therapy in patients with sickle cell disease.

    Science.gov (United States)

    Reid, Marion E; Halter Hipsky, Christine; Hue-Roye, Kim; Hoppe, Carolyn

    2014-04-01

    Red cell (RBC) blood group alloimmunization remains a major problem in transfusion medicine. Patients with sickle cell disease (SCD) are at particularly high risk for developing alloantibodies to RBC antigens compared to other multiply transfused patient populations. Hemagglutination is the classical method used to test for blood group antigens, but depending on the typing methods and reagents used may result in discrepancies that preclude interpretation based on serologic reactivity alone. Molecular methods, including customized DNA microarrays, are increasingly used to complement serologic methods in predicting blood type. The purpose of this study was to determine the diversity and frequency of RH alleles in African Americans and to assess the performance of a DNA microarray for RH allele determination. Two sets of samples were tested: (i) individuals with known variant Rh types and (ii) randomly selected African American donors and patients with SCD. Standard hemagglutination tests were used to establish the Rh phenotype, and cDNA- and gDNA-based analyses (sequencing, PCR-RFLP, and customized RHD and RHCE microarrays were used to predict the genotype). In a total of 829 samples (1658 alleles), 72 different alleles (40 RHD and 32 RHCE) were identified, 22 of which are novel. DNA microarrays detected all nucleotides probed, allowing for characterization of over 900 alleles. High-throughput DNA testing platforms provide a means to test a relatively large number of donors and potentially prevent immunization by changing the way antigen-negative blood is provided to patients. Because of the high RH allelic diversity found in the African American population, determination of an accurate Rh phenotype often requires DNA testing, in conjunction with serologic testing. Allele-specific microarrays offer a means to perform high-throughput donor Rh typing and serve as a valuable adjunct to serologic methods to predict Rh type. Because DNA microarrays test for only a fixed

  12. Detection of Doping with rhGH

    Institute of Scientific and Technical Information of China (English)

    Wu Mou-tian

    2009-01-01

    This presentation reviewed most articles recently pub lished in peer reviewed international scientific journals related to detection doping with rhGH. Four parts were in it: First part was the brief introduction about the background of do ping,special doping with rhGH. Second part summarized the development of "indirect method", markers approach. Third was focused on the development of "direct method", isoform approach. Finally, some potential alternatives have been discussed.

  13. Spectroscopic evidence against Rh 4d itinerant ferromagnetism in CeRh3B2

    Science.gov (United States)

    Sampathkumaran, E. V.; Kaindl, G.; Laubschat, C.; Krone, W.; Wortmann, G.

    1985-03-01

    Valence-band photoemission and IIII-edge x-ray absorption studies were performed on RRh3B2 (R = La, Ce, and Pr) compounds in order to explore the origin of the anomalously high magnetic ordering temperature of CeRh3B2. From the low density of Rh 4d states at EF and the approximate trivalency of Ce, itinerant magnetism in the Rh 4d band can be excluded. Instead, the magnetism is proposed to originate from Ce moments, enhanced by 4f hybridization with Rh d orbitals.

  14. Perioperative blood ordering optimization process using information from an anesthesia information management system.

    Science.gov (United States)

    Rinehart, Joseph B; Lee, Tiffany C; Kaneshiro, Kayleigh; Tran, Minh-Ha; Sun, Coral; Kain, Zeev N

    2016-04-01

    As part of ongoing perioperative surgical home implantation process, we applied a previously published algorithm for creation of a maximum surgical blood order schedule (MSBOS) to our operating rooms. We hypothesized that using the MSBOS we could show a reduction in unnecessary preoperative blood testing and associated costs. Data regarding all surgical cases done at UC Irvine Health's operating rooms from January 1, 2011, to January 1, 2014 were extracted from the anesthesia information management systems (AIMS). After the data were organized into surgical specialties and operative sites, blood order recommendations were generated based on five specific case characteristics of the group. Next, we assessed current ordering practices in comparison to actual blood utilization to identify potential areas of wastage and performed a cost analysis comparing the annual hospital costs from preoperative blood orders if the blood order schedule were to be followed to historical practices. Of the 19,138 patients who were categorized by the MSBOS as needing no blood sample, 2694 (14.0%) had a type and screen (T/S) ordered and 1116 (5.8%) had a type and crossmatch ordered. Of the 6073 procedures where MSBOS recommended only a T/S, 2355 (38.8%) had blood crossmatched. The cost analysis demonstrated an annual reduction in actual hospital costs of $57,335 with the MSBOS compared to historical blood ordering practices. We showed that the algorithm for development of a multispecialty blood order schedule is transferable and yielded reductions in preoperative blood product screening at our institution. © 2016 AABB.

  15. rhTPO在系统性红斑狼疮合并难治性血小板减少性紫癜治疗中的应用%Application of recombinant human thrombopoietin (rhTPO) in the treatment of refractorythrombocytopenic purpura in patients with systemic lupus erythematosus

    Institute of Scientific and Technical Information of China (English)

    罗文丰; 魏锦; 陈静; 倪勋

    2010-01-01

    目的 评价国产重组人血小板生成素(rhTPO)用于系统性红斑狼疮(SLE)合并难治性血小板减少性紫癜的可行性及安全性.方法 对12例SLE并难治性血小板减少性紫癜患者行国产rhTPO皮下注射,15 000 U/d,疗程14 d.检测用药前、用药后7、10、14 d及停药3个月后的血小板数,判定疗效及不良反应情况.结果 用药前血小板计数为(14.3±5.2)×109/L,用药后7、10、14 d分别为(37.8±9.3)×109/L、(64.2±15.6)×109/L、(84.3±25.7)×109/L,与用药前相比,P均<0.01.停药后3个月血小板计数为(53.2±15.6)×109/L,与治疗前比较,P<0.01.总有效率为83.33%,仅2例出现轻微不良反应.结论 rhTPO治疗SLE合并难治性血小板减少性紫癜疗效确切,且较为安全.

  16. A geographic information system for the study of past epidemics: The 1705 epidemic in Martigues (Bouches-du-Rhône, France

    Directory of Open Access Journals (Sweden)

    Stéfan Tzortzis

    2012-01-01

    Full Text Available At the beginning of the 18th century, the Provence region was hit by several severe epidemics whose causes are still not clearly understood.To draw up epidemic profiles and to identify the pathogenic agents concerned, we constituted a large onomastic database and built ageographic information system for Martigues, a medium-sized community in the south of France. The cross-linking of epidemiological,spatial and demographical data allows us to propose a new diagnosis for the epidemic which reached Martigues in the autumn of 1705.

  17. Rh nanoparticles supported on ultrathin carbon nanosheets for high-performance oxygen reduction reaction and catalytic hydrogenation.

    Science.gov (United States)

    Lin, Chong; Wu, Guanghao; Li, Huiqin; Geng, Yanmin; Xie, Gang; Yang, Jianhui; Liu, Bin; Jin, Jian

    2017-02-02

    We reported a facile and scalable salt-templated approach to produce monodisperse Rh nanoparticles (NPs) on ultrathin carbon nanosheets with the assistance of calcination under inert gas. More importantly, in spite of the essentially poor ORR activity of Rh/C, the acquired Rh/C hybrid nanosheets display a comparable ORR activity to the optimal commercial Pt/C catalyst, which may be due to the extra-small size of Rh NPs and the 2D defect-rich amorphous carbon nanosheets that can facilitate the charge transfer and reactive surface exposure. Moreover, Rh/C nanosheets present the optimal current density and best durability with the minimum decline during the entire test, so that ∼93% activity after 20 000 s is achieved, indicating a good lifetime for ORR. In contrast, commercial Pt/C and commercial Rh/C exhibited worse durability, so that ∼74% and ∼85% activities after 20 000 s are maintained. What's more, in the model system of reduction of 4-nitrophenol (4-NP), the kinetic constant k for Rh/C nanosheets is 3.1 × 10(-3), which is 4.5 times than that of the commercial Rh/C catalyst, revealing that our Rh/C hybrid nanosheets can be potentially applied in industrial catalytic hydrogenation. This work opens a novel and facile way for the rest of the precious metal NPs to be supported on ultrathin carbon nanosheets for heterogeneous catalysis.

  18. Au-Rh and Au-Pd nanocatalysts supported on rutile titania nanorods: structure and chemical stability.

    Science.gov (United States)

    Konuspayeva, Zere; Afanasiev, Pavel; Nguyen, Thanh-Son; Di Felice, Luca; Morfin, Franck; Nguyen, Nhat-Tai; Nelayah, Jaysen; Ricolleau, Christian; Li, Z Y; Yuan, Jun; Berhault, Gilles; Piccolo, Laurent

    2015-11-14

    Au, Rh, Pd, Au-Rh and Au-Pd nanoparticles (NPs) were synthesized by colloidal chemical reduction and immobilized on hydrothermally-prepared rutile titania nanorods. The catalysts were characterized by aberration-corrected TEM/STEM, XPS, and FTIR, and were evaluated in the hydrogenation of tetralin in the presence of H2S. Oxidizing and reducing thermal treatments were employed to remove the polyvinyl alcohol (PVA) surfactant. Reduction in H2 at 350 °C was found efficient for removing the PVA while preserving the size (ca. 3 nm), shape and bimetallic nature of the NPs. While Au-Pd NPs are alloyed at the atomic scale, Au-Rh NPs contain randomly distributed single-phase domains. Calcination-reduction of Au-Rh NPs mostly leads to separated Au and Rh NPs, while pre-reduction generates a well-defined segregated structure with Rh located at the interface between Au and TiO2 and possibly present around the NPs as a thin overlayer. Both the titania support and gold increase the resistance of Rh and Pd to oxidation. Furthermore, although detrimental to tetralin hydrogenation initial activity, gold stabilizes the NPs against surface sulfidation in the presence of 50 ppm H2S, leading to increased catalytic performances of the Au-Rh and Au-Pd systems as compared to their Rh and Pd counterparts.

  19. [Meta-analysis of blood system adverse events of Tripterygium wilfordii].

    Science.gov (United States)

    Li, Zhi-xia; Ma, Dong-mei; Yang, Xing-hua; Sun, Feng; Yu, Kai; Zhan, Si-yan

    2015-01-01

    A systematic review was undertaken, including studies that evaluated the incidence of the blood system adverse events of Tripterygium wilfordii (TWP). Medline, Embase and the Cochrane library were searched for relevant studies, including RCT, cohort studies and case series, of patients treated with TWP published in English and Chinese from inception up until May 25th, 2013 with the keywords including "Tripterygium wilfordii", "toxicity", "reproductive", "side effect", "adverse", "safety" and "tolerability". Relevant information was extracted and the incidence of the blood system adverse events was pooled with MetaAnalyst software. Besides, subgroup and sensitivity analyses were performed based on age, mode of medicine, observation time and disease system. According to inclusion and exclusion criteria, a total of 49 articles were included in the meta-analysis, they were split into 54 researches incorporated in the analysis. There is a large degree of heterogeneity among the studies, so data was analyzed using random-effects model and the summary estimates of incidence of the blood system adverse events was 6.1%. The weighted combined incidence of three major blood system adverse events were white-blood cells decreasing 5.6% (95% CI, 4.3% - 7.3%), hemoglobin decreasing 1.7% (95% CI, 0.5% - 5.0%) and platelet decreasing 1.8% (95% CI, 1.0% - 3.1%), respectively . Sensitivity analyses based on 45 studies with high quality showed the combined value was close to the summary estimate of total 54 studies. The current evidence indicates that the incidence of the blood system adverse events induced by TWP was high; attentions should be paid on to the prevention and treatment of the blood system adverse events.

  20. Prevention and management of RhD isoimmunization.

    Science.gov (United States)

    Harkness, Ursula F; Spinnato, Joseph A

    2004-12-01

    An Rh-negative woman is at risk for developing Rh isoimmunization upon exposure to RhD antigens from her Rh-positive baby through fetal-maternal hemorrhage. The incidence of Rh isoimmunization and fetal hemolytic disease has decreased substantially since Rh immune globulin was introduced in 1968. When RhD sensitization does occur, careful follow-up of these mothers and judicious intervention can result in good outcomes for most pregnancies. Both Doppler assessment of middle cerebral artery peak systolic velocity and spectral analysis of amniotic fluid at 450 nm (DeltaOD 450) are useful in the diagnosis and management of fetal anemia.

  1. Effect of intensive or extensive systems on buffalo heifers performances: blood metabolite values

    Directory of Open Access Journals (Sweden)

    A. Borghese

    2010-02-01

    Full Text Available Blood nutritional indicators were measured on twenty-six prepubertal 8- mo-old buffalo heifers, weighing a mean of 134 kg to determine the effect of different feeding and management systems. Animals were randomly assigned to an intensive feeding (n=13; IF group or to a pasture system plus hay (n=13; PS group, according to weight and age. Measurements of live weight and blood collection began on day 0 (=date of onset of trial.The heifers were weighed monthly, up to puberty, to evaluate the growth rate and blood samples were withdrawn every three months to assay the concentrations of blood metabolites. Significantly higher (P0.05.

  2. Blood compounds irradiation process: assessment of absorbed dose using Fricke and Thermoluminescent dosimetric systems

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Gabriela de Amorim; Squair, Peterson Lima; Pinto, Fausto Carvalho; Belo, Luiz Claudio Meira; Grossi, Pablo Andrade [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN-CNEN/MG), Belo Horizonte, MG (Brazil)], e-mail: gas@cdtn.br, e-mail: pls@cdtn.br, e-mail: fcp@cdtn.br, e-mail: lcmb@cdtn.br, e-mail: pabloag@cdtn.br

    2009-07-01

    The assessment of gamma absorbed doses in irradiation facilities allows the quality assurance and control of the irradiation process. The liability of dose measurements is assign to the metrological procedures adopted including the uncertainty evaluation. Fricke and TLD 800 dosimetric systems were used to measure absorbed dose in the blood compounds using the methodology presented in this paper. The measured absorbed doses were used for evaluating the effectiveness of the irradiation procedure and the gamma dose absorption inside the irradiation room of a gamma irradiation facility. The radiation eliminates the functional and proliferative capacities of donor T-lymphocytes, preventing Transfusion associated graft-versus-host disease (TA-GVHD), a possible complication of blood transfusions. The results show the applicability of such dosimetric systems in quality assurance programs, assessment of absorbed doses in blood compounds and dose uniformity assign to the blood compounds irradiation process by dose measurements in a range between 25 Gy and 100 Gy. (author)

  3. How Is Rh Incompatibility Treated?

    Science.gov (United States)

    ... often are seen in babies who have hemolytic anemia. This is because the compound forms when red blood cells break down. Rate This Content: NEXT >> Updated: January 1, 2011 Twitter Facebook YouTube Google+ SITE INDEX ACCESSIBILITY PRIVACY STATEMENT FOIA NO FEAR ACT OIG ...

  4. Searching for the optimal GnRH antagonist regimen to compare with GnRH agonists in IVF

    NARCIS (Netherlands)

    Huirne, J.A.F.

    2007-01-01

    De zoektocht naar het optimale GnRH-antagonistschema voor de vergelijking met GnRH-agonisten tijdens IVF behandelingen. Ondanks het gegeven dat in meer dan 200 klinische studies gebruik gemaakt wordt van gonadotrophine releasing hormone (GnRH) antagonisten, is het optimale GnRH- antagonistschema n

  5. A Digital Method for the Detection of Blood Flow Direction in Ultrasonic Doppler Systems

    Directory of Open Access Journals (Sweden)

    P. Acevedo–Contla.

    2010-01-01

    Full Text Available Doppler ultrasound systems are widely used to study blood flow and diagnosis of vascular diseases. An important characteristic of these systems is the ability to detect the direction of the blood flow. Most Doppler ultrasound systems apply a quadrature demodulation technique on the ultrasonic transducer output signal. Therefore additional treatment is necessary to separate forward and reverse flow signals. This work presents a digital method to convert signals in quadrature into directional signals using a Fast Fourier Transform (FFT approach. Validation of the method has been achieved using simulated Doppler ultrasound signals.

  6. Optimizing preoperative blood ordering with data acquired from an anesthesia information management system.

    Science.gov (United States)

    Frank, Steven M; Rothschild, James A; Masear, Courtney G; Rivers, Richard J; Merritt, William T; Savage, Will J; Ness, Paul M

    2013-06-01

    The maximum surgical blood order schedule (MSBOS) is used to determine preoperative blood orders for specific surgical procedures. Because the list was developed in the late 1970s, many new surgical procedures have been introduced and others improved upon, making the original MSBOS obsolete. The authors describe methods to create an updated, institution-specific MSBOS to guide preoperative blood ordering. Blood utilization data for 53,526 patients undergoing 1,632 different surgical procedures were gathered from an anesthesia information management system. A novel algorithm based on previously defined criteria was used to create an MSBOS for each surgical specialty. The economic implications were calculated based on the number of blood orders placed, but not indicated, according to the MSBOS. Among 27,825 surgical cases that did not require preoperative blood orders as determined by the MSBOS, 9,099 (32.7%) had a type and screen, and 2,643 (9.5%) had a crossmatch ordered. Of 4,644 cases determined to require only a type and screen, 1,509 (32.5%) had a type and crossmatch ordered. By using the MSBOS to eliminate unnecessary blood orders, the authors calculated a potential reduction in hospital charges and actual costs of $211,448 and $43,135 per year, respectively, or $8.89 and $1.81 per surgical patient, respectively. An institution-specific MSBOS can be created, using blood utilization data extracted from an anesthesia information management system along with our proposed algorithm. Using these methods to optimize the process of preoperative blood ordering can potentially improve operating room efficiency, increase patient safety, and decrease costs.

  7. Automatic system for blood donors control with seropositive results in epidemiological tests.

    Directory of Open Access Journals (Sweden)

    Ariel Menéndez Barrios

    2008-04-01

    Full Text Available Background: Prevention of infectious diseases transmission through blood transfusion is one of the biggest challenges in the transfusion field of medicine. Objective: to design a computerized system for blood donors control with seropositive results in epidemiological tests. Methods: A system designed by the Provincial Blood Bank using Microsoft Access as a data base system and DELFHI 7 as associated language. It runs under Windows 2000 or any other superior system with 64 RAM and do not need special requirements of software or hardware. It includes the following data: Full name, age, sex, race, Id number, home address, number of the clinical record in the blood bank, date when the trial took place in each patient, kind of disease diagnosed, technical data of the trial such as: fluorescence, sectional level of the 1st trial as well as the repetition, name of the technician who performed it. Results: It permits to consult general data of donors as well as the disease diagnosed. It also permits to elaborate reports of these patients from any PC of the blood bank acceding with the security password of the program. Conclusions: It constitutes a beneficial tool which permits to improve the notification system established in the program of the web about sexually transmitted diseases from the Minister of Public Health in Cuba.

  8. LH response to GnRH blood test

    Science.gov (United States)

    ... as release of too much hormone ( hyperprolactinemia ) Large pituitary tumors Decrease in hormones made by the endocrine glands Too much iron in the body ( hemochromatosis ) Eating disorders, such as anorexia Recent significant weight loss, such ...

  9. Short-term administration of rhGH increases markers of cellular proliferation but not milk protein gene expression in normal lactating women

    OpenAIRE

    Maningat, Patricia D.; Sen, Partha; Rijnkels, Monique; Hadsell, Darryl L.; Bray, Molly S.; Haymond, Morey W.

    2011-01-01

    Growth hormone is one of few pharmacologic agents known to augment milk production in humans. We hypothesized that recombinant human GH (rhGH) increases the expression of cell proliferation and milk protein synthesis genes. Sequential milk and blood samples collected over four days were obtained from five normal lactating women. Following 24 h of baseline milk and blood sampling, rhGH (0.1 mg/kg/day) was administered subcutaneously once daily for 3 days. Gene expression changes were determine...

  10. [Solid phase techniques in blood group serology].

    Science.gov (United States)

    Uthemann, H; Sturmfels, L; Lenhard, V

    1993-06-01

    As alternatives to hemagglutination, solid-phase red blood cell adherence assays are of increasing importance. The adaptation of the new techniques to microplates offers several advantages over hemagglutination. Using microplates the assays may be processed semiautomatically, and the results can be read spectrophotometrically and interpreted by a personal computer. In this paper, different red blood cell adherence assays for AB0 grouping, Rh typing, Rh phenotyping, antibody screening and identification, as well as crossmatching will be described.

  11. Stability and ordering properties of fcc alloys based on Rh, Ir, Pd, and Pt

    Science.gov (United States)

    Turchi, P. E. A.; Drchal, V.; Kudrnovský, J.

    2006-08-01

    Stability properties and ordering trends for the six face-centered cubic binary combinations of the four transition metals Rh, Ir, Pd, and Pt are examined in the context of electronic structure calculations. The method is based on a Green’s function description of the electronic structure of random alloys. Configurational order is treated within the generalized perturbation method. On one hand, the three alloys Pd-Rh, Pd-Ir, and Pt-Ir that have been studied experimentally are confirmed to behave like phase-separating systems. On the other hand, the other three mixtures Pd-Pt, Rh-Ir, and Pt-Rh, for which phase-separating trends have been inferred from experiments, are found to display chemical order with ordering of the (1 0 0) and (11/20) family types and a mixture of both, respectively. The origin of these results is discussed in terms of electronic structure properties.

  12. The development of a recruiting-drawing-inventory model for a community blood bank system.

    Science.gov (United States)

    Smackey, B M

    1975-01-01

    A community blood bank system is a multiorganizational program that is designed to supply the blood needs of a community. Participating in such a program are hospitals, a central blood bank, industrial donor groups, the American Red Cross, advisory committees, and the community at large. The underlying determinant of the community's success or failure with its blood program is the degree of cooperation among the various organizations. Intertwined with organizational considerations are the management problems associated with the operation of a responsive and efficient inventory control system. This paper reports on the development of a system for a community blood bank that is in its third year of operation. The system that has been developed can be operated manually by a part-time clerk. Details of the model include an integration of the donor scheduling function and the inventory control function. Simulated testing of the model has been conducted and full-scale implementation is awaiting the expansion of the known donor base.

  13. Simultaneous pulmonary and systemic blood pressure and ECG Interval measurement in conscious, freely moving rats.

    Science.gov (United States)

    Rey, Markus; Weber, Edgar W; Hess, Patrick D

    2012-03-01

    Here we evaluated the ability of a new, dual blood-pressure telemetry transmitter to simultaneously measure pulmonary and systemic blood pressure and the electrocardiogram in rats. The transmitter was implanted in normotensive and monocrotaline-induced pulmonary hypertensive Wistar rats, with sensing catheters placed in the pulmonary artery (channel 1) and descending aorta (channel 2). Biopotential electrodes were positioned to record an apex-based lead II electrocardiogram. Pulmonary and systemic arterial blood pressure and electrocardiographic waveforms were recorded between 2 and 12 wk after implantation of the transmitter. During this period, pulmonary arterial pressure progressively increased in monocrotaline-treated compared with saline-treated rats. The pharmacologic response of rats to reference compounds was measured by using the transmitter to validate the technique and to evaluate the ability of the device to transmit changes in blood pressure and the electrocardiogram. Validation against 2 Millar high-fidelity blood-pressure catheters confirmed the accuracy of the blood pressure data recorded with the transmitter. In addition, local tolerance of the associated catheters was confirmed by histologic examination.

  14. Drug Delivery Systems, CNS Protection, and the Blood Brain Barrier

    Directory of Open Access Journals (Sweden)

    Ravi Kant Upadhyay

    2014-01-01

    Full Text Available Present review highlights various drug delivery systems used for delivery of pharmaceutical agents mainly antibiotics, antineoplastic agents, neuropeptides, and other therapeutic substances through the endothelial capillaries (BBB for CNS therapeutics. In addition, the use of ultrasound in delivery of therapeutic agents/biomolecules such as proline rich peptides, prodrugs, radiopharmaceuticals, proteins, immunoglobulins, and chimeric peptides to the target sites in deep tissue locations inside tumor sites of brain has been explained. In addition, therapeutic applications of various types of nanoparticles such as chitosan based nanomers, dendrimers, carbon nanotubes, niosomes, beta cyclodextrin carriers, cholesterol mediated cationic solid lipid nanoparticles, colloidal drug carriers, liposomes, and micelles have been discussed with their recent advancements. Emphasis has been given on the need of physiological and therapeutic optimization of existing drug delivery methods and their carriers to deliver therapeutic amount of drug into the brain for treatment of various neurological diseases and disorders. Further, strong recommendations are being made to develop nanosized drug carriers/vehicles and noninvasive therapeutic alternatives of conventional methods for better therapeutics of CNS related diseases. Hence, there is an urgent need to design nontoxic biocompatible drugs and develop noninvasive delivery methods to check posttreatment clinical fatalities in neuropatients which occur due to existing highly toxic invasive drugs and treatment methods.

  15. [Evaluation of the blood coagulation system after surgeries on abdominal aortic aneurysms].

    Science.gov (United States)

    Nikul'nikov, P I; Liksunov, O V; Ratushniuk, A V; Lugovs'koĭ, E V; Kolesnikova, I M; Lytvynova, L M; Kostiuchenko, O P; Chernyshenko, T M; Hornyts'ka, O V; Platonova, T M

    2012-09-01

    Basing on data of analysis of the hemostasis system state in the patients, suffering abdominal aorta aneurysm, a tendency for raising of postoperative soluble fibrin and D-dimer content in the blood plasm and reduction of these indices on the third day was noted. The abovementioned markers content depends on the aneurysm size, the fibrin deposits presence, the terms from clinical signs beginning to the certain therapy administration and anticoagulants application. Information about correlation between content of D-dimer and soluble fibrin in the treatment dynamics is important for determination of activation degree in the patients blood coagulation system and the thrombotic complications prognosis.

  16. Conjecture on reflectionlessness of blood-vascular system as a wave-conducting medium

    Science.gov (United States)

    Goldobin, D. S.; Mizeva, I. A.

    2017-06-01

    Our research is related to the employment of photoplethysmography (PPG) and laser Doppler flowmetry (LDF) techniques (measuring the blood volume and flux, respectively) for the peripheral vascular system. We derive the governing equations of the wave dynamics for the case of extremely inhomogeneous parameters. We argue for the conjecture that the blood-vascular system as a wave-conducting medium should be nearly reflection-free. With the reflectionlessness condition, one can find the general solution to the governing equation and, on the basis of this solution, analyse the relationships between PPG- and LDF-signals.

  17. Exploring the Role of La Codoping beyond Charge Compensation for Enhanced Hydrogen Evolution by Rh-SrTiO3.

    Science.gov (United States)

    Modak, Brindaban; Ghosh, Swapan K

    2015-08-27

    In this theoretical study, we investigate recent observation of enhancement of hydrogen evolution efficiency of Rh-doped SrTiO3 due to codoping with La at the Sr lattice site. Using hybrid density functional theory, we have systematically studied the electronic structure of (Rh, La)-codoped SrTiO3 and compared with that of Rh-doped SrTiO3, La-doped SrTiO3, and undoped SrTiO3. The aim of the present study has been to explore the role of different factors toward the observed enhanced photoactivity of (Rh, La)-codoped SrTiO3. Doping with only Rh significantly reduces the photoabsorption energy by introducing localized acceptor states between the valence band and conduction band. Unfortunately, these states act as efficient sources for charge carrier trapping. Besides, the oxygen vacancy found to be present in the Rh-doped SrTiO3 as a charge compensating defect also accelerates the electron-hole recombination rate. We have shown that codoping with La and Rh leads to the formation of clean band structure without encountering any midgap states. Introduction of La into the Rh-doped SrTiO3 not only reduces the quantity of Rh(4+) species but also suppresses the oxygen vacancy due to formation of a charge-compensated system. The presence of La favors Rh doping into the crystal structure of SrTiO3 by reducing the formation energy. Moreover, the conduction band minima are found to be shifted in the upward direction significantly due to codoping with Rh and La, thereby increasing the reducing behavior at the conduction band. This leads to enhancement of hydrogen evolution activity of SrTiO3 during photocatalytic water splitting under visible light.

  18. Interlayer coupling of orthogonally magnetized (Rh/Fe{sub 1-x}Co{sub x}){sub N}/Rh(001) thin films

    Energy Technology Data Exchange (ETDEWEB)

    Yildiz, Fikret; Przybylski, Marek; Kirschner, Juergen [Max-Planck-Institut fuer Mikrostrukturphysik, Halle (Germany)

    2009-07-01

    We report on fully epitaxial (Rh/Fe{sub 1-x}Co{sub x}){sub N}/Rh(001) exchange-coupled multilayer system in which every other magnetic layer (Fe{sub 1-x}Co{sub x}, 0.4Rh(001) originates from an appropriate tetragonal distortion and varies with the film composition with a maximum around x=0.5. The most attractive advantage of the system is that the magnetic anisotropy can be continuously tuned by varying the alloy film composition. The magnetic layers are separated by Rh non-magnetic spacers which mediate a ferro- or antiferromagnetic exchange coupling depending on the thickness The Rh spacer layers support the distortion and thus the perpendicular easy magnetization axis up to tens of MLs. In reality the magnetization does not alternate between out-of-plane for the Fe{sub 1-x}Co{sub x} and in-plane for the Fe (or Co) layers since the interlayer exchange interaction tends to orient the magnetization of both layers in parallel.

  19. Ammonia secretion from fish gill depends on a set of Rh glycoproteins.

    Science.gov (United States)

    Nakada, Tsutomu; Westhoff, Connie M; Kato, Akira; Hirose, Shigehisa

    2007-04-01

    Ammonia excretion from the gill in teleost fish is essential for nitrogen elimination. Although numerous physiological studies have measured ammonia excretion, the mechanism of ammonia movement through the membranes of gill epithelial cells is still unknown. Mammalian Rh glycoproteins are members of a family of proteins that mediate ammonia transport in bacteria, yeast, and plants. We identified the Rh glycoprotein homologs, fRhag, fRhbg, fRhcg1, and fRhcg2, of the pufferfish, Takifugu rubripes. Northern blot, in situ hybridization, and immunohistochemistry revealed that the pufferfish erythroid Rh glycoprotein homologue fRhag was present in red blood cells and the hematological organs (spleen and kidney) in fish. All four pufferfish Rh glycoproteins are specifically localized in the gill and line the pillar cells, pavement cells, and the mitochondrion-rich cells. Heterologous expression in Xenopus oocytes showed that they mediate methylammonium (an analog of ammonium) transport. These results suggest that pufferfish Rh glycoproteins are involved in ammonia excretion from the gill. These findings challenge the classic view that ammonia excretion in the fish gill occurs by passive diffusion.

  20. Performance Evaluation and Labeling Comprehension of a New Blood Glucose Monitoring System with Integrated Information Management

    Science.gov (United States)

    List, Susan M; Starks, Nykole; Baum, John; Greene, Carmine; Pardo, Scott; Parkes, Joan L; Schachner, Holly C; Cuddihy, Robert

    2011-01-01

    Background This study evaluated performance and product labeling of CONTOUR® USB, a new blood glucose monitoring system (BGMS) with integrated diabetes management software and a universal serial bus (USB) port, in the hands of untrained lay users and health care professionals (HCPs). Method Subjects and HCPs tested subject's finger stick capillary blood in parallel using CONTOUR USB meters; deep finger stick blood was tested on a Yellow Springs Instruments (YSI) glucose analyzer for reference. Duplicate results by both subjects and HCPs were obtained to assess system precision. System accuracy was assessed according to International Organization for Standardization (ISO) 15197:2003 guidelines [within ±15 mg/dl of mean YSI results (samples <75 mg/dl) and ±20% (samples ≥75 mg/dl)]. Clinical accuracy was determined by Parkes error grid analysis. Subject labeling comprehension was assessed by HCP ratings of subject proficiency. Key system features and ease-of-use were evaluated by subject questionnaires. Results All subjects who completed the study (N = 74) successfully performed blood glucose measurements, connected the meter to a laptop computer, and used key features of the system. The system was accurate; 98.6% (146/148) of subject results and 96.6% (143/148) of HCP results exceeded ISO 15197:2003 criteria. All subject and HCP results were clinically accurate (97.3%; zone A) or associated with benign errors (2.7%; zone B). The majority of subjects rated features of the BGMS as “very good” or “excellent.” Conclusions CONTOUR USB exceeded ISO 15197:2003 system performance criteria in the hands of untrained lay users. Subjects understood the product labeling, found the system easy to use, and successfully performed blood glucose testing. PMID:22027308

  1. [The use of blood and its components in two gynecological-obstetrical wards of selected Silesian hospitals in years 1996-2002].

    Science.gov (United States)

    Dylag, Stanisław; Kucharz, Eugeniusz Józef

    2011-03-01

    Blood and its components are valuable medication that should be administered according to recommendation after consideration of expected benefits and adverse reactions to the patient. To analyze amount and form of blood components or whole blood transfused at gynecological-obstetrical wars of university hospital (UH) or regional hospital (RH). Age of the patients and clinical diagnosis were included into the analysis. Two gynecological-obstetrical wards were investigated. The appropriate data were obtained from the hospital documentation (there was no electronic data system). Amount and form of transfused blood components or whole blood, diagnosis at admission, patient's age and number of the treated patients were collected from the hospital transfusion book. The final clinical diagnosis, age of the patient, and transfusion details were obtained from the patient's medical records. The diagnoses were presented according to the ICD-10 classification. UH patients (29,759 patients) and 13,540 patients from RH from 1996 to 2002 were investigated. Blood and its components were used for transfusion in 1150 women (3.8%) treated in UH, and in 206 women (1.5%) hospitalized in RH. In years 1996-2002, there were transfused 2746 units of blood and its components, including: 371 units of whole blood (13.5%), 2073 units of red blood cells (75.5%), 281 units of fresh frozen plasma (10.2%) and 21 units of therapeutic platelet concentrate (0.8%) in RH there were transfused 527 units of blood and its components, including: 8 units of whole blood (1.5%), 450 units of red blood cells (85.4%), 63 units of fresh frozen plasma (12%), and 6 units of therapeutic platelet concentrate (1.1%). The age of patients in UH was between 14 and 92 years and in RH between 18 and 79 years. The rate of patients with the same diagnosis in whom the transfusion was made, was higher in University Hospital. There were no differences between the number of transfused blood and its components between the two

  2. Relationship between Serum Iron Profile and Blood Groups among the Voluntary Blood Donors of Bangladesh.

    Science.gov (United States)

    Hoque, M M; Adnan, S D; Karim, S; Al-Mamun, M A; Faruki, M A; Islam, K; Nandy, S

    2016-04-01

    ferritin and percentage transferring saturation in different ABO and Rh blood grouping categories. Blood donors with blood group O had lowest haemoglobin, serum iron and transferring saturation levels and donors with blood group A had highest TIBC level. Blood donors with blood group B had lowest serum ferritin level. The understanding of the different blood groups ability to retain iron in their system can give an insight into their ability to handle the disease iron deficiency anaemia.

  3. Epigenetic control of GnRH neurons

    Directory of Open Access Journals (Sweden)

    Joseph Raymond Kurian

    2013-05-01

    Full Text Available Epigenetic modifications to the genome, including DNA methylation and histone modifications, occur in response to external stimuli. Reproductive function is highly sensitive to environmental conditions including season, diet, hormonal changes, and exposure to chemical contaminants. GnRH neurons, which play a key role in reproduction, are particularly sensitive to various environmental stimuli. We recently reported that the rhesus monkey GnRH gene exhibits distinct epigenetic differentiation during embryonic development. More recently, we further found that a similar epigenetic phenomenon occurs across puberty. In this article, we highlight recent findings, suggest implications of these findings (or potential mechanisms and then discuss current challenges as well as future work. Consequently, this review will provide background to understand the epigenetic control of GnRH neurons as a link between the environment and reproductive function.

  4. Research of quaternized chitosan based hybrid scaffold as a delivery system for rhBMP-2%壳聚糖季铵盐基杂化支架的制备及其作为rhBMP-2载体的研究*

    Institute of Scientific and Technical Information of China (English)

    胡园园; 王靖; 刘昌胜

    2014-01-01

    Quaternized chitosan was synthesized by the reaction of chitosan and glycidyltrimethylammonium chloride (GTMAC)and named as N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride (HTCC). A hydrogel system composed of HTCC andβ-glycerophosphate (β-GP)was obtained by intermolecular forces. Introduction ofβ-TCP granules improved the mechanical strength and controlled the swelling behavior and pro-longed the releasing period for rhBMP-2 of the hydrogel scaffold.Moreover,rhBMP-2 loaded scaffolds were as-sessed in ectopic bone formation.The results indicate that porous HTCC/β-GP/β-TCP/rhBMP-2 scaffolds are promising candidates for application in tissue engineering of bone.%采用环氧衍生物开环法制备壳聚糖季铵盐,通过其与甘油磷酸钠的分子间作用力交联成凝胶,冻干后得多孔支架,用于装载骨形态发生蛋白-2应用于骨修复领域;同时引入β-TCP 作为物理交联点,在提高支架力学强度的同时,更好地调控支架的溶胀行为,延长rhBMP-2的释放时间,并且在异位诱导成骨的动物实验中取得良好效果。

  5. Expression of manganese superoxide dismutase in rat blood, heart and brain during induced systemic hypoxia

    Directory of Open Access Journals (Sweden)

    Septelia I. Wanandi

    2011-02-01

    Full Text Available Background: Hypoxia results in an increased generation of ROS. Until now, little is known about the role of MnSOD - a major endogenous antioxidant enzyme - on the cell adaptation response against hypoxia. The aim of this study was to  determine the MnSOD mRNA expression and levels of specific activity in blood, heart and brain of rats during induced systemic hypoxia.Methods: Twenty-five male Sprague Dawley rats were subjected to systemic hypoxia in an hypoxic chamber (at 8-10% O2 for 0, 1, 7, 14 and 21 days, respectively. The mRNA relative expression of MnSOD was analyzed using Real Time RT-PCR. MnSOD specific activity was determined using xanthine oxidase inhibition assay.Results: The MnSOD mRNA relative expression in rat blood and heart was decreased during early induced systemic hypoxia (day 1 and increased as hypoxia continued, whereas the mRNA expression in brain was increased since day 1 and reached its maximum level at day 7. The result of MnSOD specific activity during early systemic hypoxia was similar to the mRNA expression. Under very late hypoxic condition (day 21, MnSOD specific activity in blood, heart and brain was significantly decreased. We demonstrate a positive correlation between MnSOD mRNA expression and specific activity in these 3 tissues during day 0-14 of induced systemic hypoxia. Furthermore, mRNA expression and specific activity levels in heart strongly correlate with those in blood.Conclusion: The MnSOD expression at early and late phases of induced systemic hypoxia is distinctly regulated. The MnSOD expression in brain differs from that in blood and heart revealing that brain tissue can  possibly survive better from induced systemic hypoxia than heart and blood. The determination of MnSOD expression in blood can be used to describe its expression in heart under systemic hypoxic condition. (Med J Indones 2011; 20:27-33Keywords: MnSOD, mRNA expression, ROS, specific activity, systemic hypoxia

  6. A systems-based approach to managing blood pressure in children following kidney transplantation.

    Science.gov (United States)

    Hooper, David K; Mitsnefes, Mark

    2016-10-01

    Hypertension is one of the most common and well-known complications following kidney transplantation in children. Yet, despite numerous available therapies many pediatric kidney transplant recipients continue to have poorly controlled blood pressure, suggesting that traditional approaches to blood pressure management in this population might be inadequate. Over the last two decades, the Chronic Care Model has been developed to improve chronic illness outcomes through delivery system design and clinical information systems that support patient self-management and provider decision-making. In this educational review we discuss key elements of managing blood pressure following pediatric kidney transplantation and suggest ways that they may be reliably implemented into clinical practice using principles from the Chronic Care Model.

  7. An intelligent system for continuous blood pressure monitoring on remote multi-patients in real time

    CERN Document Server

    Marani, Roberto

    2012-01-01

    In this paper we present an electronic system to perform a non-invasive measurement of the blood pressure based on the oscillometric method, which does not suffer from the limitations of the well-known auscultatory one. Moreover the proposed system is able to evaluate both the systolic and diastolic blood pressure values and makes use of a microcontroller and a Sallen-Key active filter. With reference to other similar devices, a great improvement of our measurement system is achieved since it performs the transmission of the systolic and diastolic pressure values to a remote computer. This aspect is very important when the simultaneous monitoring of multi-patients is required. The proposed system, prototyped and tested at the Electron Devices Laboratory (Electrical and Information Engineering Department) of Polytechnic University of Bari, Italy, is characterized by originality, by plainness of use and by a very high level of automation (so called intelligent system).

  8. [The genetic differentiation of the human populations of Donetsk Province, Ukraine. The distribution of ABO and Rhesus blood group systems].

    Science.gov (United States)

    Mukhin, V N

    1994-01-01

    We have detected the irregular genetic-population structure of residents of the Donetsk Province with respect to the AB0 and Rh systems with a general formula A > 0 > B > AB, the only exception being Slavyansk where it probably is 0 > A > B > AB. Two out of nine populations studied differed greatly from the others. The two other populations exhibited a high degree of similarity with a distant population in Russia (Novocherkassk) that can be attributed to the common history of their population.

  9. NO Decomposition on Rh Supported CNTs

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The decomposition of NO has been studied in a steady flow reactor on fresh carbon nanotubes (CNTs), purified CNTs, Rh/p-CNTs and Rh/g-Al2O3 between 573 K and 973 K. NO decomposition at 573 K was initially complete over a reduced surface. The amount of NO absorption increased at temperature below 773 K. 100% NO conversion and more than 100% N2 activity was achieved at 973 K for 150 min on p-CNTs and f-CNTs.

  10. Magnetic moment distribution of ferromagnetic Ni--Rh alloys

    Energy Technology Data Exchange (ETDEWEB)

    Cable, J.W.; Wollan, E.O.

    1977-01-01

    The diffuse scattering of polarized and unpolarized neutrons was used to determine the spatial distribution of the magnetic moment for ferromagnetic Ni-Rh alloys. The average Ni moment remains near 0.6 ..mu../sub B/ to 12 at. % Rh and then decreases toward zero at the critical concentration of 37 at. % Rh. There is an initial rapid decrease in the Rh moment that follows a P/sub 12/ dependence and corresponds to a moment of 2 ..mu../sub B/ for isolated Rh atoms. The data indicate moment fluctuations at both the Ni and the Rh sites that are associated with local environment.

  11. Compliance with postpartum Rh isoimmunization prophylaxis in Alberta

    OpenAIRE

    1985-01-01

    A retrospective review of obstetric records for 1979 in two major Calgary hospitals was undertaken to determine the rate of compliance with postpartum Rh isoimmunization prophylaxis in Alberta. The charts of 4528 women ranging in age from 13 to 46 years were reviewed. The prevalence rate of Rh negativity was found to be 16%. Of the 710 Rh-negative women 490 (69%) were eligible to receive Rh immune globulin (RhIG); that is, they had no anti-D antibodies, and the baby/fetus was Rh-positive or R...

  12. Catalytic reactions on neutral Rh oxide clusters more efficient than on neutral Rh clusters.

    Science.gov (United States)

    Yamada, Akira; Miyajima, Ken; Mafuné, Fumitaka

    2012-03-28

    Gas phase catalytic reactions involving the reduction of N(2)O and oxidation of CO were observed at the molecular level on isolated neutral rhodium clusters, Rh(n) (n = 10-28), using mass spectrometry. Sequential oxygen transfer reactions, Rh(n)O(m-1) + N(2)O → Rh(n)O(m) + N(2) (m = 1, 2, 3,…), were monitored and the rate constant for each reaction step was determined as a function of the cluster size. Oxygen extraction reactions by a CO molecule, Rh(n)O(m) + CO → Rh(n)O(m-1) + CO(2) (m = 1, 2, 3,…), were also observed when a small amount of CO was mixed with the reactant N(2)O gas. The rate constants of the oxygen extraction reactions by CO for m ≥ 4 were found to be two or three orders of magnitude higher than the rate constants for m ≤ 3, which indicates that the catalytic reaction proceeds more efficiently when the reaction cycles turn over around Rh(n)O(m) (m ≥ 4) than around bare Rh(n). Rhodium clusters operate as more efficient catalysts when they are oxidized than non- or less-oxidized rhodium clusters, which is consistent with theoretical and experimental studies on the catalytic CO oxidation reaction on a rhodium surface.

  13. Mechanism and kinetics of the NOCO reaction on Rh

    Science.gov (United States)

    Zhdanov, V. P.; Kasemo, B.

    During the past 15 years, the NOCO reaction on Rh has attracted considerable attention of the researchers working in academic and applied surface science. The practical importance of this reaction is connected with its relevance for environmental chemistry. From the point of view of academic studies, the NOCO reaction on Rh is of interest because it represents one of the simplest examples from the class of catalytic reactions occurring via decomposition of adsorbed species. At present, the detailed kinetic data for this reaction are available both for single-crystal and supported Rh, at ultrahigh vacuum (UHV) conditions and also at realistic pressures. For this reason, the NOCO reaction on Rh has become one of the major testing platforms for a microscopic, surface-science based approach to heterogeneous catalysis. The present review shows how far the progress in this field has come. In particular, the review describes in detail the evolution of the ideas for the mechanism of the reaction and also presents the data for the elementary reaction steps, obtained primarily on Rh(1 1 1) at UHV conditions. Then, the possibility of using these data for simulation of the reaction kinetics at moderate pressures, P NO ⋍ P CO ⋍ 0.01 bar, is discussed. The technological aspects of application of Rh in the automotive exhaust systems are surveyed as well, but only briefly.

  14. Ferromagnetism and unconventional impurity effects in Rh- and Ga-doped LaCoO3

    Science.gov (United States)

    Asai, Shinichiro; Okazaki, Ryuji; Terasaki, Ichiro; Yasui, Yukio

    2014-03-01

    The perovskite oxide LaCoO3 has been long investigated because of a dramatic change of its spin state for Co3+ ions with temperature variation. The Co3+ ions (3d6) in LaCoO3 takes the non-magnetic low-spin state of t 2g6at low temperature. The spin state of the Co3+ ions in this system is sensitive to the chemical substitutions; we have found a weak ferromagnetism in a solid solution of LaCoO3 and LaRhO3 [S. Asai et al., JPSJ 80, 104705 (2011).]. Since the two oxides are non-magnetic at low temperature, our finding is an example of ``order by disorder,'' where a non-magnetic impurity makes a non-magnetic oxide ferromagnetic. We have further investigated the magnetization and x-ray diffraction of LaCo0 . 8 - yRh0.2MyO3 (M = Rh, Ga) [S. Asai et al., PRB 86, 014421 (2012).]. The magnetization decreases by the Ga3+ substitution much more drastically than by the Rh3+ substitution. It indicates that at least two kinds of Co3+ ions exist in LaCo0.8Rh0.2O3; one is nonmagnetic, and the other is magnetic. In this talk, we will also discuss the change of the lattice volume with the Rh3+ and Ga3+ substitution [S. Asai et al., JPSJ 82, 114606 (2013).].

  15. [Diagnosis and therapy in fetuses at risk in Rh isoimmunization].

    Science.gov (United States)

    Calda, P; Zizka, Z; Rezábek, K; Masata, J; Bendl, J

    1993-12-01

    The objective of the work was to evaluate the importance of antenatal examination of amniotic fluid and foetal blood in case of suspected Rh isoimmunization of the foetus. In 1991-1992 in 16 patients with a rise of the titre of anti-D antibodies to > 1:8 between the 24th and 36th week of gestation 32 punctures of the umbilicus by means of a 22 gauge needle were made under continual ultrasonic control. In two instances intraumbilical transfusion was indicated. The authors revealed that with the rising titre of anti-D antibodies in maternal blood the foetal haematocrit value in the umbilical blood declines. With the rising bilirubin level the haematocrit declines. In foetuses with a haematocrit of < 31% severe forms of jaundice are encountered more frequently with the necessity of long-term phototherapy and exchange transfusion. The authors did not find a correlation between the haematocrit of foetal blood and the bilirubinoid concentration in amniotic fluid, assessed by Liley's method. Foetuses with a haematocrit higher than 31% are not threatened by severe forms of jaundice and therefore the authors do not use transfusions in these foetuses. Based on hitherto assembled experience, the authors confirmed that cordocentesis is associated with a comparable risk as amniocentesis but provides more accurate information on the state of the foetus.

  16. Polycystic ovary syndrome, blood group & diet: A correlative study in South Indian females

    Directory of Open Access Journals (Sweden)

    Rahul Pal, Pratik Kumar Chatterjee, Poulomi Chatterjee, Vinodini NA, PrasannaMithra, Sourjya Banerjee, Suman VB2, Sheila R. Pai

    2014-07-01

    Full Text Available Aim: To find out the co-relation between polycystic ovary syndrome (PCOS with blood group & diet in South Indian females, between the age-group of (20-30 years. Objectives: Correlative analysis of ABO & Rh system, dietary habits & alcohol consumption with PCOS. Materials & Methods: 100 patients between (20-30 years, diagnosed with PCOS were selected. A standard PCOS questionnaire was given. Blood group & dietary status data were collected. Patients were grouped according to ABO & Rh system considering their diet & alcohol intake (p≤0.05 significant. Result: Our data revealed that the highest risk of PCOS was observed in females with blood group ‘O’ positive followed by ‘B’ positive who were on mixed diet & used to consume alcohol. Our study also suggests that Rh negative individuals didn’t show any association with PCOS. Conclusion: The results of our study suggest that ‘O’ positive females, are more prone to PCOS. Though the relative frequency of B positive individuals are more in India, females with blood group O positive are more susceptible to PCOS, contributing factors being mixed diet & alcohol intake. So, early screening of ‘O’ positive &‘B’ positive females of reproductive age-group in South-India, could be used as a measure for timely diagnosis of PCOS, better management &also prevention of complications. However, further research should be done to investigate the multifaceted mechanisms triggering these effects.

  17. The effects of transport by pneumatic tube system on blood cell count, erythrocyte sedimentation and coagulation tests

    OpenAIRE

    Koçak, Fatma Emel; Yöntem, Mustafa; Yücel, Özlem; Çilo, Mustafa; Genç, Özlem; Meral, Ayfer

    2013-01-01

    Introduction: Today, the pneumatic tube transport system (PTS) is used frequently because of its advantages related to timing and speed. However, the impact of various types of PTS on blood com-ponents is unknown. The aim of this study was to examine the influence of PTS on the quality of rou-tine blood cell counts, erythrocyte sedimentation, and certain blood coagulation tests. Materials and methods: Paired blood samples were obtained from each of 45 human volunteers and evaluated by bloo...

  18. Non-association between anti-Toxoplasma gondii antibodies and ABO blood group system

    Directory of Open Access Journals (Sweden)

    ACF Rodrigues

    2011-01-01

    Full Text Available Toxoplasma gondii infects humans through the gastrointestinal tract (GIT, which elicits humoral immune response with specific antibodies. The expression of the ABO blood group glycoconjugates also occurs in this same system and may influence the human susceptibility of infection by T. gondii. The aim of the present study was to investigate the association between ABO blood group phenotypes and the presence of anti-T. gondii antibodies. Data - including age, results of serology tests for T. gondii infection and ABO blood group phenotypes - were assembled from the medical records of 1,006 pregnant women attended in the Base Hospital of the Medical School of São José do Rio Preto, Brazil, between 2001 and 2004. The chi-square test was used to compare the results with the level of significance set at 5%. Of the studied cases, 64.1% (645/1006 and 35.9% (391/1006 presented respectively positive and negative serology tests for anti-T. gondii antibodies. The mean age of those who tested positive was higher than those with negative serology tests (p = 0.0004. The frequencies of ABO blood group phenotypes were similar in those with and without anti-T. gondii antibodies (p = 0.35. In conclusion, the ABO blood group system is not associated with the presence or absence of anti-T. gondii antibodies.

  19. Blood feeding of Ornithodoros turicata larvae using an artificial membrane system

    Science.gov (United States)

    An artificial membrane system was adapted to feed Ornithodoros turicata larvae from a laboratory colony using defibrinated swine blood. Aspects related to larval feeding and molting to the 1st nymphal instar were evaluated. Fifty-five percent of all larvae exposed to the artificial membrane in two e...

  20. The immune system mediates blood-brain barrier damage; Possible implications for pathophysiology of neuropsychiatric illnesses

    NARCIS (Netherlands)

    VanderWerf, YD; DeJongste, MJL; terHorst, GJ

    1995-01-01

    The immune system mediates blood-brain barrier damage; possible implications for pathophysiology of neuropsychiatric illnesses. In this investigation the effects of immune activation on the brain are characterized In order to study this, we used a model for chronic immune activation, the myocardial

  1. An Analysis of Pulsed Wave Ultrasound Systems for Blood Velocity Estimation

    DEFF Research Database (Denmark)

    Jensen, J. A.

    1995-01-01

    Pulsed wave ultrasound systems can be used for determining blood's velocity non-invasively in the body. A region of interest is selected, and the received signal is range gated to measure data from the region. One complex sample value is acquired for each pulse emission after complex demodulation...

  2. Improvement of castable refractories for RH snorkel; RH shinshitsukanyo futeikeizai no kaizen

    Energy Technology Data Exchange (ETDEWEB)

    Nishi, K.; Obana, T.; Fijii, T.; Shimizu, I. [Harima Ceramics Corp., Hyogo (Japan)

    1999-11-01

    Relating to corrosion of RH submerged nozzle, resistance against iron oxide of monolithic refractories was examined. Corrosion and seepage of refractories were measured by rotating corrosion, refractories include alumina-spinel castable, which is applied to RH under tank, and alumina-magnesia castable, which is generally used for RH, submerged nozzle. Alumina-spinel castable is superior in resistance against iron oxide than alumina-magnesia castable, and the resistance decreased with increase of stainless fiber addition to castable. Alumina-spinel castable without stainless fiber was suitable for bottom end of the dipping pipe. (NEDO)

  3. A Post-Marketing Surveillance Study to Evaluate Performance of the EXIMO™ Blood Glucose Monitoring System.

    Science.gov (United States)

    Chandnani, Sonia R; Ramakrishna, C D; Dave, Bhargav A; Kothavade, Pankaj S; Thakkar, Ashok S

    2017-05-01

    The performance of Blood Glucose Monitoring System (BGMS) is critical as the information provided by the system guide the patient or health care professional in making treatment decisions. However, besides evaluating accuracy of the BGMS in laboratory setting, it is equally important that the intended users (healthcare professionals and patients) should be able to achieve blood glucose measurements with similar level of high accuracy. To assess the performance of EXIMO™ (Meril Diagnostics Pvt. Ltd., Vapi, Gujarat, India) BGMS as per International Organization for Standardization (ISO) 15197:2013 section 8 user performance criteria. This was a non-randomized and post-marketing study conducted at a tertiary care centre of India. A total of 1005 patients with diabetes themselves performed fingertip blood glucose measurement using EXIMO™ BGMS. Immediately after capillary blood glucose measurement using the blood glucose monitoring system, venous blood sample from each patient was obtained by a trained technician which was assessed by reference laboratory method- Cobas Integra 400 plus (Roche Instrument Centre, Rotkreuz, Switzerland). All the blood glucose measurements assessed by EXIMO™ were compared with laboratory results. Performance of the system was assessed as per ISO 15197:2013 criteria using Bland-Altman plot, Parkes-Consensus Error Grid (CEG) and Surveillance Error Grid analyses (SEG). A total of 1005 patients participated in the study. Average age of the patients was 44.93±14.65 years. Evaluation of capillary fingertip blood glucose measurements demonstrated that 95.82% measurements fulfilled ISO 15197:2013 section 8 user performance criteria. All the results lie within clinically non-critical zones; Zone A (99.47%; n=1000) and Zone B (0.53%; n=05) of the CEG analysis. As per SEG analysis, majority of the results fell within "no-risk" zone (risk score 0 to 0.5; 90.42%). The result of the study confirmed that intended users are able to obtain accurate

  4. Solvent-dependent interconversions between Rh(I), Rh(II), and Rh(III) complexes of an aryl-monophosphine ligand.

    Science.gov (United States)

    Montag, Michael; Leitus, Gregory; Shimon, Linda J W; Ben-David, Yehoshoa; Milstein, David

    2007-01-01

    Reaction of the aryl-monophosphine ligand alpha(2)-(diisopropylphosphino)isodurene (1) with the Rh(I) precursor [Rh(coe)(2)(acetone)(2)]BF(4) (coe=cyclooctene) in different solvents yielded complexes of all three common oxidation states of rhodium, depending on the solvent used. When the reaction was carried out in methanol a cyclometalated, solvent-stabilized Rh(III) alkyl-hydride complex (2) was obtained. However, when the reaction was carried out in acetone or dichloromethane a dinuclear eta(6)-arene Rh(II) complex (5) was obtained in the absence of added redox reagents. Moreover, when acetonitrile was added to a solution of either the Rh(II) or Rh(III) complexes, a new solvent-stabilized, noncyclometalated Rh(I) complex (6) was obtained. In this report we describe the different complexes, which were fully characterized, and probe the processes behind the remarkable solvent effect observed.

  5. A case of severe Rh (D) alloimmunization pregnant woman delivery an infant with limited treatment.

    Science.gov (United States)

    Xu, Wenhao

    2013-10-01

    A 35-year-old woman with histories of frequent failed pregnancies was pregnant after having five plasma exchange procedures during which she was given Rh (D) positive plasma as replacement and her anti-D antibody titer went from 512 to 1024. Antenatal surveillance of the fetus showed no abnormality. At 36 weeks gestation she delivered an infant who initially had no significant clinical problems but was severely anemic on the following days. Using exchange transfusion and blood transfusions the infant's hemoglobin was normal at 4 months of age. Thus, the Rh (D) status of donor plasma should be considered when used as the replacement in plasma exchange for Rh (D) negative women. Severe Rh (D) alloimmunization pregnant woman may delivery an infant who seem in good condition at birth. If severe Rhesus isoimmunisation of the infant is confirmed, whole blood exchange should be done as early as possible and the infant must be considered to be at risk for late anemia. Clinical judgment plays a vital role in the decision to transfuse red cells or not. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Performance of two updated blood glucose monitoring systems: an evaluation following ISO 15197:2013.

    Science.gov (United States)

    Pleus, Stefan; Baumstark, Annette; Rittmeyer, Delia; Jendrike, Nina; Haug, Cornelia; Freckmann, Guido

    2016-05-01

    Objective For patients with diabetes, regular self-monitoring of blood glucose (SMBG) is essential to ensure adequate glycemic control. Therefore, accurate and reliable blood glucose measurements with SMBG systems are necessary. The international standard ISO 15197 describes requirements for SMBG systems, such as limits within which 95% of glucose results have to fall to reach acceptable system accuracy. The 2013 version of this standard sets higher demands, especially regarding system accuracy, than the currently still valid edition. ISO 15197 can be applied by manufacturers to receive a CE mark for their system. Research design and methods This study was an accuracy evaluation following ISO 15197:2013 section 6.3 of two recently updated SMBG systems (Contour * and Contour TS; Bayer Consumer Care AG, Basel, Switzerland) with an improved algorithm to investigate whether the systems fulfill the requirements of the new standard. For this purpose, capillary blood samples of approximately 100 participants were measured with three test strip lots of both systems and deviations from glucose values obtained with a hexokinase-based comparison method (Cobas Integra † 400 plus; Roche Instrument Center, Rotkreuz, Switzerland) were determined. Percentages of values within the acceptance criteria of ISO 15197:2013 were calculated. This study was registered at clinicaltrials.gov (NCT02358408). Main outcome Both updated systems fulfilled the system accuracy requirements of ISO 15197:2013 as 98.5% to 100% of the results were within the stipulated limits. Furthermore, all results were within the clinically non-critical zones A and B of the consensus error grid for type 1 diabetes. Conclusions The technical improvement of the systems ensured compliance with ISO 15197 in the hands of healthcare professionals even in its more stringent 2013 version. Alternative presentation of system accuracy results in radar plots provides additional information with certain advantages. In addition

  7. Role of histaminergic system in blood-brain barrier dysfunction associated with neurological disorders.

    Science.gov (United States)

    Bañuelos-Cabrera, Ivette; Valle-Dorado, María Guadalupe; Aldana, Blanca Irene; Orozco-Suárez, Sandra Adela; Rocha, Luisa

    2014-11-01

    Blood-brain barrier (BBB) disruption has been associated with several acute and chronic brain disorders such as Alzheimer's disease, Parkinson's disease and epilepsy. This represents a critical situation because damaged integrity of the BBB is related to the influx of immune mediators, plasma proteins and other outside elements from blood to the central nervous system (CNS) that may trigger a cascade of events that leads to neuroinflammation. In this review, evidence that mast cells and the release of factors such as histamine play an important role in the neuroinflammatory process associated with brain disorders such as Alzheimer's disease, Parkinson's disease and epilepsy is presented.

  8. The Diabetes Assistant: A Smartphone-Based System for Real-Time Control of Blood Glucose

    OpenAIRE

    Patrick Keith-Hynes; Benton Mize; Antoine Robert; Jérôme Place

    2014-01-01

    Type 1 Diabetes Mellitus (T1DM) is an autoimmune disease in which the insulin-producing beta cells of the pancreas are destroyed and insulin must be injected daily to enable the body to metabolize glucose. Standard therapy for T1DM involves self-monitoring of blood glucose (SMBG) several times daily with a blood glucose meter and injecting insulin via a syringe, pen or insulin pump. An “Artificial Pancreas” (AP) is a closed-loop control system that uses a continuous glucose monitor (CGM), a...

  9. Modeling of human colonic blood flow for a novel artificial anal sphincter system

    Institute of Scientific and Technical Information of China (English)

    Peng ZAN; Guo-zheng YAN; Hua LIU

    2008-01-01

    A novel artificial anal sphincter system has been developed to simulate the normal physiology of the human anorectum. With the goal of engineering a safe and reliable device, the model of human colonic blood flow has been built and the relationship between the colonic blood flow rate and the operating occlusion pressure of the anorectum is achieved. The tissue ischemia is analyzed based on constitutive relations for human anorectum. The results suggest that at the planned operating occlusion pressure of less than 4 kPa the artificial anal sphincter should not risk the vaseularity of the human colon.

  10. Evolving Trends: Hyperbilirubinemia Among Newborns Delivered to Rh Negative Mothers in Southern India

    Science.gov (United States)

    N, Girish; S, Santosh; SR, Keshavamurthy

    2013-01-01

    Introduction: Neonatal jaundice is the commonest abnormal physical finding in the new born nursery and hemolytic disease of the newborn (HDN) among babies born to Rh negative mothers is the most formidable etiology. During last few decades considerable evolution has been observed in this entity secondary to development of several novel preventive, diagnostic and therapeutic modalities. Objective: To study the current trends in presentation, management and outcome of hyperbilirubinemia among newborns delivered to Rh negative mothers. Methodology: This observational descriptive study with prospective data collection included one hundred live born term babies born to Rh negative mothers in our hospital. A predesigned proforma was used to record antenatal and postnatal data .Cord blood collected during delivery for assessment of bilirubin,hematocrit and direct coombs test.Serum bilirubin levels were estimated in babies with clinical jaundice and treated for the same if required.All babies were regularly followed up weekly for one month. Chi square test/Fisher Exact test and Student “t” test has been used to find the significant association of jaundice(incidence,treatment) and study characteristics. Results: Out of 100 babies enrolled, 57 babies developed jaundice. Jaundice is 2.7 times more likely associated with babies born to multiparous Rh-ve mothers with p=0.017*. Jaundice is 3 times more likely associated with Rh+ve babies born to multiparous mothers with p=0.020*. Jaundice is 3.97 times more likely associated with Rh+ve babies born to multiparous mothers who have not received Anti-D with p=0.154. Treatment of jaundice is 2.75 times more likely in Rh+ve babies born to multiparous mothers who have not received Anti-D with p=0.162. Duration of phototherapy is significantly more in Rh+ve babies born to multiparous mothers who had not received Anti-D with p=0.0097*.Exchange transfusion was required in two babies. Conclusion: Although the incidence of Rh

  11. Evolving trends: hyperbilirubinemia among newborns delivered to rh negative mothers in southern India.

    Science.gov (United States)

    N, Girish; S, Santosh; Sr, Keshavamurthy

    2013-11-01

    Neonatal jaundice is the commonest abnormal physical finding in the new born nursery and hemolytic disease of the newborn (HDN) among babies born to Rh negative mothers is the most formidable etiology. During last few decades considerable evolution has been observed in this entity secondary to development of several novel preventive, diagnostic and therapeutic modalities. To study the current trends in presentation, management and outcome of hyperbilirubinemia among newborns delivered to Rh negative mothers. This observational descriptive study with prospective data collection included one hundred live born term babies born to Rh negative mothers in our hospital. A predesigned proforma was used to record antenatal and postnatal data .Cord blood collected during delivery for assessment of bilirubin,hematocrit and direct coombs test.Serum bilirubin levels were estimated in babies with clinical jaundice and treated for the same if required.All babies were regularly followed up weekly for one month. Chi square test/Fisher Exact test and Student "t" test has been used to find the significant association of jaundice(incidence,treatment) and study characteristics. Out of 100 babies enrolled, 57 babies developed jaundice. Jaundice is 2.7 times more likely associated with babies born to multiparous Rh-ve mothers with p=0.017*. Jaundice is 3 times more likely associated with Rh+ve babies born to multiparous mothers with p=0.020*. Jaundice is 3.97 times more likely associated with Rh+ve babies born to multiparous mothers who have not received Anti-D with p=0.154. Treatment of jaundice is 2.75 times more likely in Rh+ve babies born to multiparous mothers who have not received Anti-D with p=0.162. Duration of phototherapy is significantly more in Rh+ve babies born to multiparous mothers who had not received Anti-D with p=0.0097*.Exchange transfusion was required in two babies. Although the incidence of Rh isoimmunization has declined dramatically over the years ,it is still an

  12. Atomic and molecular adsorption on Rh(111)

    DEFF Research Database (Denmark)

    Mavrikakis, Manos; Rempel, J.; Greeley, Jeffrey Philip

    2002-01-01

    A systematic study of the chemisorption of both atomic (H, O, N, S, C), molecular (N-2, CO, NO), and radical (CH3, OH) species on Rh(111) has been performed. Self-consistent, periodic, density functional theory (DFT-GGA) calculations, using both PW91 and RPBE functionals, have been employed to de...

  13. Noninvasive fetal RhD genotyping

    DEFF Research Database (Denmark)

    Clausen, Frederik Banch; Damkjær, Merete Berthu; Dziegiel, Morten Hanefeld

    2014-01-01

    Immunization against RhD is the major cause of hemolytic disease of the fetus and newborn (HDFN), which causes fetal or neonatal death. The introduction of postnatal immune prophylaxis in the 1960s drastically reduced immunization incidents in pregnant, D-negative women. In several countries...

  14. Pt、Rh及Pt-Rh合金电极上氢的吸附%Hydrogen Adsorption on Pt, Rh and Pt-Rh Electrodes

    Institute of Scientific and Technical Information of China (English)

    贾梦秋; A.M.Meretskyi

    2005-01-01

    The hydrogen adsorption on Pt-Rh alloys in sulfuric acid aqueous solutions was studied by the method of cathode pulses. Hydrogen adsorption on the electrode with all ratio of alloy components (wRh = 0-100%) is well described by the Temkin logarithmic isotherm. The surface coverage by adsorbed hydrogen at the same potential is decreased with increasing content of rhodium in the system. A linear dependence of adsorption peak potential on the alloy compositions in the case of weakly bonded adsorbed hydrogen is established. Hydrogen adsorption heat as a function of surface coverage for Pt-Rh-electrodes was obtained. The shape of the current-potential curve and position of the weakly bonded hydrogen adsorption on the potential scale are all related to alloy compositions, thus can serve as the basis for the determination surface composition of alloys.

  15. Recombinant human antithrombin III: rhATIII.

    Science.gov (United States)

    2004-01-01

    GTC Biotherapeutics (formerly Genzyme Transgenics Corporation) is developing a transgenic form of antithrombin III known as recombinant human antithrombin III [rhATIII]. It is produced by inserting human DNA into the cells of goats so that the targeted protein is excreted in the milk of the female offspring. The transgenic goats have been cloned in collaboration with the Louisiana State University Agriculture Center. GTC Biotherapeutics is conducting clinical trials of rhATIII in coagulation disorders. rhATIII is believed to be both safer and more cost-effective than the currently available plasma-derived product. rhATIII is also being investigated in cancer and acute lung injury. Genzyme Transgenics Corporation, originally a subsidiary of Genzyme Corporation, changed its name to GTC Biotherapeutics in June 2002; it is no longer a subsidiary of Genzyme Corporation. GTC Biotherapeutics is seeking partners for the commercialisation of rhATIII. Restructuring of GTC Biotherapeutics to support its commercialisation programmes was announced in February 2004. Genzyme Transgenics Corporation was developing rhATIII in association with Genzyme General (Genzyme Corporation) in the ATIII LLC joint venture, but in November 2000 a letter of intent was signed for the reacquisition of the rights by Genzyme Transgenics Corporation. It was announced in February 2001 that this reacquisition was not going to be completed and that the development of rhATIII was to continue with ATIII LLC. However, in July 2001, Genzyme Transgenics Corporation reacquired all the rights in the transgenic antithrombin III programme. SMI Genzyme Ltd, a joint venture between Sumitomo Metal Industries, Japan, and Genzyme Transgenics Corporation, USA, was set up to fund development of transgenic antithrombin III in Asia. However, in October 2000, Genzyme Transgenics Corporation reacquired, from Sumitomo Metal Industries, the rights to its technology for production of medicines from milk in 18 Asian countries

  16. Computational Exploration of Rh(III)/Rh(V) and Rh(III)/Rh(I) Catalysis in Rhodium(III)-Catalyzed C-H Activation Reactions of N-Phenoxyacetamides with Alkynes.

    Science.gov (United States)

    Yang, Yun-Fang; Houk, K N; Wu, Yun-Dong

    2016-06-01

    The selective rhodium-catalyzed functionalization of arenes is greatly facilitated by oxidizing directing groups that act both as directing groups and internal oxidants. We report density functional theory (B3LYP and M06) investigations on the mechanism of rhodium(III)-catalyzed redox coupling reaction of N-phenoxyacetamides with alkynes. The results elucidated the role of the internal oxidizing directing group, and the role of Rh(III)/Rh(I) and Rh(III)/Rh(V) catalysis of C-H functionalizations. A novel Rh(III)-Rh(V)-Rh(III) cycle successfully rationalizes recent experimental observations by Liu and Lu et al. ( Liu , G. Angew. Chem. Int. Ed. 2013 , 52 , 6033 ) on the reactions of N-phenoxyacetamides with alkynes in different solvents. Natural Bond Orbital (NBO) analysis confirms the identity of Rh(V) intermediate in the catalytic cycle.

  17. Rh Factor: How It Can Affect Your Pregnancy

    Science.gov (United States)

    ... Education & Events Advocacy For Patients About ACOG The Rh Factor: How It Can Affect Your Pregnancy Home ... Your Pregnancy FAQ027, September 2013 PDF Format The Rh Factor: How It Can Affect Your Pregnancy Pregnancy ...

  18. Evaluation of a transcutaneous blood gas monitoring system in critically ill dogs.

    Science.gov (United States)

    Holowaychuk, Marie K; Fujita, Hiroshi; Bersenas, Alexa M E

    2014-01-01

    To describe the use of a transcutaneous blood gas monitoring system in critically ill dogs, determine if transcutaneous and arterial blood gas values have good agreement, and verify if clinical or laboratory variables are correlated with differences between transcutaneous and arterial blood gas measurements. Prospective observational study. University teaching hospital ICU. Twenty-three client-owned dogs. In critically ill dogs undergoing arterial blood gas monitoring, a transcutaneous blood gas monitor was used to measure transcutaneous partial pressure of carbon dioxide (PtcCO2 ) and transcutaneous partial pressure of oxygen (PtcO2 ) values 30 minutes after sensor placement, which were compared to PaCO2 and PaO2 values measured simultaneously. Clinical and laboratory variables were concurrently recorded to determine if they were correlated with the difference between transcutaneous and arterial blood gas measurements. Bland-Altman analysis revealed a mean bias of 4.6 ± 26.3 mm Hg (limits of agreement [LOA]: -46.9/+56.1 mm Hg) between PtcO2 and PaO2 and a mean bias of 9.3 ± 8.5 mm Hg (LOA: -7.5/+26.0 mm Hg) between PtcCO2 and PaCO2 . The difference between PtcCO2 -PaCO2 was strongly negatively correlated with HCO3 (-) (r(2) = 0.52, P blood pressure (r(2) = 0.21, P = 0.044), whereas the difference between PtcCO2 -PaCO2 was moderately negatively correlated with diastolic blood pressure (r(2) = 0.33, P = 0.008). Agreement between transcutaneous and arterial PO2 and PCO2 measurements in these critically ill dogs was inferior to that reported in similar adult and pediatric human studies. The transcutaneous monitor consistently over-estimated PaO2 and PaCO2 and should not be used to replace arterial blood gas measurements in critically ill dogs requiring blood gas interpretation. © Veterinary Emergency and Critical Care Society 2014.

  19. {sup 103}Rh NMR investigation of the superconductor Rh{sub 17}S{sub 15}

    Energy Technology Data Exchange (ETDEWEB)

    Koyama, T., E-mail: t-koyama@sci.u-hyogo.ac.j [Graduate School of Material Science, University of Hyogo, Kamigori, Hyogo 678-1297 (Japan); Kanda, K.; Motoyama, G.; Ueda, K.; Mito, T.; Kohara, T. [Graduate School of Material Science, University of Hyogo, Kamigori, Hyogo 678-1297 (Japan); Nakamura, H. [Department of Materials Science and Engineering, Kyoto University, Kyoto 606-8501 (Japan)

    2010-12-15

    We present {sup 103}Rh NMR studies for the superconductor Rh{sub 17}S{sub 15} (T{sub c} 5.4 K). We have identified the observed NMR lines corresponding to four different Rh sites in the cubic unit cell and deduced the temperature (T) dependence of the Knight shift components in Rh 24m site whose point symmetry is not axial. The isotropic part of the Knight shift K decreases with T in the normal state, indicating the negative hyperfine coupling and the enhancement of the spin susceptibility at lower T. The sudden change of K below T{sub c} is an indication of the spin-singlet Cooper paring.

  20. Carotid arterial blood pressure waveform monitoring using a portable ultrasound system.

    Science.gov (United States)

    Joohyun Seo; Pietrangelo, Sabino J; Hae-Seung Lee; Sodini, Charles G

    2015-08-01

    This work presents a non-invasive arterial blood pressure (ABP) waveform monitoring technique using ultrasound. A portable ultrasound system to excite ultrasound transducers and acquire data is designed with off-the-shelf components. The insonation angles are identified using a vector Doppler technique based on the cosine dependency of the Doppler signals. The pulse pressure of an estimated waveform at the left common carotid artery is compared to the standard sphygmomanometer measurement in a clinical test. The estimated carotid ABP waveform shows excellent agreement to the finger ABP waveform with expected discrepancy of the systolic peak shape due to different measurement sites. The proposed method also tracks slow blood pressure fluctuations. This validation on human subjects shows potential for a noninvasive blood pressure waveform monitoring device at central arterial sites.

  1. Immunophenotyping of Lymphocyte T and B in the Peripheral Blood of Systemic Lupus Erythematosus

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The immunophenotyping expression levels of lymphocyte in the peripheral blood from 21 patients with active systemic lupus erythematosus (SLE) were analyzed by using the immunofluorescence labeling-flow cytometry technique to investigate the immunophenotyping expression of lymphocytes T and B in the peripheral blood of active SLE patients and its clinical value. It was showed that, compared with normal controls, the expression of CD+3, CD+4 and the ratio of CD+4/CD+8 in the peripheral blood of these patients were decreased (P<0.01), while the expression of CD+8, CD+20 was significantly increased (P<0.01). It was suggested that both T and B cells in patients with active SLE involved in immunoregulation, were activated. The abnormal expression of lymphocyte immunophenotyping could influence the immune reaction in SLE patients, which might be one of the important pathogenesis factors in SLE.

  2. Novel optoacoustic system for noninvasive continuous monitoring of cerebral venous blood oxygenation

    Science.gov (United States)

    Petrov, Yuriy; Petrov, Irene Y.; Prough, Donald S.; Esenaliev, Rinat O.

    2012-02-01

    Traumatic brain injury (TBI) and spinal cord injury are a major cause of death for individuals under 50 years of age. In the USA alone, 150,000 patients per year suffer moderate or severe TBI. Moreover, TBI is a major cause of combatrelated death. Monitoring of cerebral venous blood oxygenation is critically important for management of TBI patients because cerebral venous blood oxygenation below 50% results in death or severe neurologic complications. At present, there is no technique for noninvasive, accurate monitoring of this clinically important variable. We proposed to use optoacoustic technique for noninvasive monitoring of cerebral venous blood oxygenation by probing cerebral veins such as the superior sagittal sinus (SSS) and validated it in animal studies. In this work, we developed a novel, medical grade optoacoustic system for continuous, real-time cerebral venous blood oxygenation monitoring and tested it in human subjects at normal conditions and during hyperventilation to simulate changes that may occur in patients with TBI. We designed and built a highly-sensitive optoacoustic probe for SSS signal detection. Continuous measurements were performed in the near infrared spectral range and the SSS oxygenation absolute values were automatically calculated in real time using a special algorithm developed by our group. Continuous measurements performed at normal conditions and during hyperventilation demonstrated that hyperventilation resulted in approximately 12% decrease of cerebral venous blood oxygenation.

  3. Application of Blood Appointment System in Hospital%血液预约系统在医院的应用

    Institute of Scientific and Technical Information of China (English)

    王飞; 李刚荣; 蒋天伦

    2011-01-01

    目的:通过血液预约,缓解血液供需关系.方法:应用现代信息技术手段,通过血液信息管理系统与医院信息管理系统的无缝连接及互联