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Sample records for replication control system

  1. Controlled Experiment Replication in Evaluation of E-Learning System's Educational Influence

    Science.gov (United States)

    Grubisic, Ani; Stankov, Slavomir; Rosic, Marko; Zitko, Branko

    2009-01-01

    We believe that every effectiveness evaluation should be replicated at least in order to verify the original results and to indicate evaluated e-learning system's advantages or disadvantages. This paper presents the methodology for conducting controlled experiment replication, as well as, results of a controlled experiment and an internal…

  2. CRISPR-mediated control of the bacterial initiation of replication

    NARCIS (Netherlands)

    Wiktor, J.M.; Lesterlin, Christian; Sherratt, David J.; Dekker, C.

    2016-01-01

    Programmable control of the cell cycle has been shown to be a powerful tool in cell-biology studies. Here, we develop a novel system for controlling the bacterial cell cycle, based on binding of CRISPR/dCas9 to the origin-of-replication locus. Initiation of replication of bacterial chromosomes is

  3. DNA Replication Control During Drosophila Development: Insights into the Onset of S Phase, Replication Initiation, and Fork Progression

    Science.gov (United States)

    Hua, Brian L.; Orr-Weaver, Terry L.

    2017-01-01

    Proper control of DNA replication is critical to ensure genomic integrity during cell proliferation. In addition, differential regulation of the DNA replication program during development can change gene copy number to influence cell size and gene expression. Drosophila melanogaster serves as a powerful organism to study the developmental control of DNA replication in various cell cycle contexts in a variety of differentiated cell and tissue types. Additionally, Drosophila has provided several developmentally regulated replication models to dissect the molecular mechanisms that underlie replication-based copy number changes in the genome, which include differential underreplication and gene amplification. Here, we review key findings and our current understanding of the developmental control of DNA replication in the contexts of the archetypal replication program as well as of underreplication and differential gene amplification. We focus on the use of these latter two replication systems to delineate many of the molecular mechanisms that underlie the developmental control of replication initiation and fork elongation. PMID:28874453

  4. CRISPR-mediated control of the bacterial initiation of replication.

    Science.gov (United States)

    Wiktor, Jakub; Lesterlin, Christian; Sherratt, David J; Dekker, Cees

    2016-05-05

    Programmable control of the cell cycle has been shown to be a powerful tool in cell-biology studies. Here, we develop a novel system for controlling the bacterial cell cycle, based on binding of CRISPR/dCas9 to the origin-of-replication locus. Initiation of replication of bacterial chromosomes is accurately regulated by the DnaA protein, which promotes the unwinding of DNA at oriC We demonstrate that the binding of CRISPR/dCas9 to any position within origin or replication blocks the initiation of replication. Serial-dilution plating, single-cell fluorescence microscopy, and flow-cytometry experiments show that ongoing rounds of chromosome replication are finished upon CRISPR/dCas9 binding, but no new rounds are initiated. Upon arrest, cells stay metabolically active and accumulate cell mass. We find that elevating the temperature from 37 to 42°C releases the CRISR/dCas9 replication inhibition, and we use this feature to recover cells from the arrest. Our simple and robust method of controlling the bacterial cell cycle is a useful asset for synthetic biology and DNA-replication studies in particular. The inactivation of CRISPR/dCas9 binding at elevated temperatures may furthermore be of wide interest for CRISPR/Cas9 applications in genomic engineering. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  5. Modelling the Replication Management in Information Systems

    Directory of Open Access Journals (Sweden)

    Cezar TOADER

    2017-01-01

    Full Text Available In the modern economy, the benefits of Web services are significant because they facilitates the activities automation in the framework of Internet distributed businesses as well as the cooperation between organizations through interconnection process running in the computer systems. This paper presents the development stages of a model for a reliable information system. This paper describes the communication between the processes within the distributed system, based on the message exchange, and also presents the problem of distributed agreement among processes. A list of objectives for the fault-tolerant systems is defined and a framework model for distributed systems is proposed. This framework makes distinction between management operations and execution operations. The proposed model promotes the use of a central process especially designed for the coordination and control of other application processes. The execution phases and the protocols for the management and the execution components are presented. This model of a reliable system could be a foundation for an entire class of distributed systems models based on the management of replication process.

  6. Iterated function systems for DNA replication

    Science.gov (United States)

    Gaspard, Pierre

    2017-10-01

    The kinetic equations of DNA replication are shown to be exactly solved in terms of iterated function systems, running along the template sequence and giving the statistical properties of the copy sequences, as well as the kinetic and thermodynamic properties of the replication process. With this method, different effects due to sequence heterogeneity can be studied, in particular, a transition between linear and sublinear growths in time of the copies, and a transition between continuous and fractal distributions of the local velocities of the DNA polymerase along the template. The method is applied to the human mitochondrial DNA polymerase γ without and with exonuclease proofreading.

  7. A Transactional Asynchronous Replication Scheme for Mobile Database Systems

    Institute of Scientific and Technical Information of China (English)

    丁治明; 孟小峰; 王珊

    2002-01-01

    In mobile database systems, mobility of users has a significant impact on data replication. As a result, the various replica control protocols that exist today in traditional distributed and multidatabase environments are no longer suitable. To solve this problem, a new mobile database replication scheme, the Transaction-Level Result-Set Propagation (TLRSP)model, is put forward in this paper. The conflict detection and resolution strategy based on TLRSP is discussed in detail, and the implementation algorithm is proposed. In order to compare the performance of the TLRSP model with that of other mobile replication schemes, we have developed a detailed simulation model. Experimental results show that the TLRSP model provides an efficient support for replicated mobile database systems by reducing reprocessing overhead and maintaining database consistency.

  8. MYC and the Control of DNA Replication

    Science.gov (United States)

    Dominguez-Sola, David; Gautier, Jean

    2014-01-01

    The MYC oncogene is a multifunctional protein that is aberrantly expressed in a significant fraction of tumors from diverse tissue origins. Because of its multifunctional nature, it has been difficult to delineate the exact contributions of MYC’s diverse roles to tumorigenesis. Here, we review the normal role of MYC in regulating DNA replication as well as its ability to generate DNA replication stress when overexpressed. Finally, we discuss the possible mechanisms by which replication stress induced by aberrant MYC expression could contribute to genomic instability and cancer. PMID:24890833

  9. DOD Business Systems Modernization: Progress in Establishing Corporate Management Controls Needs to Be Replicated Within Military Departments

    National Research Council Canada - National Science Library

    Hite, Randolph C; Epps, Elena; Holland, Michael; Johnson, Tonia; Lakhmani, Neelaxi; LaPaze, Rebecca; Le, Anhe; Paintsil, Freda

    2008-01-01

    ..., and investment management, and continue to address the act s requirements relative to business system budgetary disclosure and certification and approval of systems costing in excess of $1 million...

  10. Optimal control of gene mutation in DNA replication.

    Science.gov (United States)

    Yu, Juanyi; Li, Jr-Shin; Tarn, Tzyh-Jong

    2012-01-01

    We propose a molecular-level control system view of the gene mutations in DNA replication from the finite field concept. By treating DNA sequences as state variables, chemical mutagens and radiation as control inputs, one cell cycle as a step increment, and the measurements of the resulting DNA sequence as outputs, we derive system equations for both deterministic and stochastic discrete-time, finite-state systems of different scales. Defining the cost function as a summation of the costs of applying mutagens and the off-trajectory penalty, we solve the deterministic and stochastic optimal control problems by dynamic programming algorithm. In addition, given that the system is completely controllable, we find that the global optimum of both base-to-base and codon-to-codon deterministic mutations can always be achieved within a finite number of steps.

  11. Physically Embedded Minimal Self-Replicating Systems

    DEFF Research Database (Denmark)

    Fellermann, Harold

    Self-replication is a fundamental property of all living organisms, yet has only been accomplished to limited extend in manmade systems. This thesis is part of the ongoing research endeavor to bridge the two sides of this gap. In particular, we present simulation results of a minimal life...... for any model above the atomistic scale. This is achieved by deriving an alternative scaling procedure for interaction parameters in the model. We perform system-level simulations of the design which attempt to account for theoretical, and experimental knowledge, as well as results from other...

  12. The Nature of Stability in Replicating Systems

    Directory of Open Access Journals (Sweden)

    Addy Pross

    2011-02-01

    Full Text Available We review the concept of dynamic kinetic stability, a type of stability associated specifically with replicating entities, and show how it differs from the well-known and established (static kinetic and thermodynamic stabilities associated with regular chemical systems. In the process we demonstrate how the concept can help bridge the conceptual chasm that continues to separate the physical and biological sciences by relating the nature of stability in the animate and inanimate worlds, and by providing additional insights into the physicochemical nature of abiogenesis.

  13. Pressure tube replication techniques using the advanced NDE system

    International Nuclear Information System (INIS)

    Isherwood, A.; Jarron, D.; Travers, J.; Hanley, K.

    2006-01-01

    Periodic and in-service inspections of fuel channels are essential for the proper assessment of the structural integrity of these vital components. The arrival of new delivery devices for fuel channel inspections has driven new tooling for gathering and analyzing NDE data. The Advanced Non-Destructive Examination (ANDE) Replication System has been designed to compliment the ANDE Inspection System by providing a two plate replica system. These plates deliver a compound that makes a positive 3D mould of known ID flaws to gather information for flaw assessment. The two plate system, and the ability to retrieve and recharge the moulds in the reactor vault allows for gathering defect information with minimal critical path time. The ANDE Replication System was built on the foundation of CIGAR experience by a solid design team familiar with 3D CAD and manufacturing techniques. The tooling and controls went through a series of integration stages in the laboratory and then later with the Universal Delivery Machine (UDM) before being used on reactor starting in 2003. Once the inspection phase of an outage has been completed, the analysis team provides a list of flaw candidates that require 'root radius' information to complete the flaw assessment. This is a measure of how sharp the corners are in the defect. This data is used as part of the stress calculation that ultimately determines how many shutdown cycles that the reactor can have before that flaw must be re-inspected. The inspection tool is then swapped out of the delivery machine in the reactor vault using the versatile connectorized umbilical. The replication tool is loaded on the machine, charged with replica compound on each of the two plates, and then sent to the target channel(s). On channel, the operators use the same console as the ANDE Inspection System, but have a separate control system with a graphical display of the tool that shows its position in the channel with respect to the E-face. The axial and

  14. Plasmid P1 replication: negative control by repeated DNA sequences.

    OpenAIRE

    Chattoraj, D; Cordes, K; Abeles, A

    1984-01-01

    The incompatibility locus, incA, of the unit-copy plasmid P1 is contained within a fragment that is essentially a set of nine 19-base-pair repeats. One or more copies of the fragment destabilizes the plasmid when present in trans. Here we show that extra copies of incA interfere with plasmid DNA replication and that a deletion of most of incA increases plasmid copy number. Thus, incA is not essential for replication but is required for its control. When cloned in a high-copy-number vector, pi...

  15. Diversity of the DNA Replication System in the Archaea Domain

    Directory of Open Access Journals (Sweden)

    Felipe Sarmiento

    2014-01-01

    Full Text Available The precise and timely duplication of the genome is essential for cellular life. It is achieved by DNA replication, a complex process that is conserved among the three domains of life. Even though the cellular structure of archaea closely resembles that of bacteria, the information processing machinery of archaea is evolutionarily more closely related to the eukaryotic system, especially for the proteins involved in the DNA replication process. While the general DNA replication mechanism is conserved among the different domains of life, modifications in functionality and in some of the specialized replication proteins are observed. Indeed, Archaea possess specific features unique to this domain. Moreover, even though the general pattern of the replicative system is the same in all archaea, a great deal of variation exists between specific groups.

  16. Multiple regulatory systems coordinate DNA replication with cell growth in Bacillus subtilis.

    Directory of Open Access Journals (Sweden)

    Heath Murray

    2014-10-01

    Full Text Available In many bacteria the rate of DNA replication is linked with cellular physiology to ensure that genome duplication is coordinated with growth. Nutrient-mediated growth rate control of DNA replication initiation has been appreciated for decades, however the mechanism(s that connects these cell cycle activities has eluded understanding. In order to help address this fundamental question we have investigated regulation of DNA replication in the model organism Bacillus subtilis. Contrary to the prevailing view we find that changes in DnaA protein level are not sufficient to account for nutrient-mediated growth rate control of DNA replication initiation, although this regulation does require both DnaA and the endogenous replication origin. We go on to report connections between DNA replication and several essential cellular activities required for rapid bacterial growth, including respiration, central carbon metabolism, fatty acid synthesis, phospholipid synthesis, and protein synthesis. Unexpectedly, the results indicate that multiple regulatory systems are involved in coordinating DNA replication with cell physiology, with some of the regulatory systems targeting oriC while others act in a oriC-independent manner. We propose that distinct regulatory systems are utilized to control DNA replication in response to diverse physiological and chemical changes.

  17. Multiple regulatory systems coordinate DNA replication with cell growth in Bacillus subtilis.

    Science.gov (United States)

    Murray, Heath; Koh, Alan

    2014-10-01

    In many bacteria the rate of DNA replication is linked with cellular physiology to ensure that genome duplication is coordinated with growth. Nutrient-mediated growth rate control of DNA replication initiation has been appreciated for decades, however the mechanism(s) that connects these cell cycle activities has eluded understanding. In order to help address this fundamental question we have investigated regulation of DNA replication in the model organism Bacillus subtilis. Contrary to the prevailing view we find that changes in DnaA protein level are not sufficient to account for nutrient-mediated growth rate control of DNA replication initiation, although this regulation does require both DnaA and the endogenous replication origin. We go on to report connections between DNA replication and several essential cellular activities required for rapid bacterial growth, including respiration, central carbon metabolism, fatty acid synthesis, phospholipid synthesis, and protein synthesis. Unexpectedly, the results indicate that multiple regulatory systems are involved in coordinating DNA replication with cell physiology, with some of the regulatory systems targeting oriC while others act in a oriC-independent manner. We propose that distinct regulatory systems are utilized to control DNA replication in response to diverse physiological and chemical changes.

  18. Multiple Regulatory Systems Coordinate DNA Replication with Cell Growth in Bacillus subtilis

    Science.gov (United States)

    Murray, Heath; Koh, Alan

    2014-01-01

    In many bacteria the rate of DNA replication is linked with cellular physiology to ensure that genome duplication is coordinated with growth. Nutrient-mediated growth rate control of DNA replication initiation has been appreciated for decades, however the mechanism(s) that connects these cell cycle activities has eluded understanding. In order to help address this fundamental question we have investigated regulation of DNA replication in the model organism Bacillus subtilis. Contrary to the prevailing view we find that changes in DnaA protein level are not sufficient to account for nutrient-mediated growth rate control of DNA replication initiation, although this regulation does require both DnaA and the endogenous replication origin. We go on to report connections between DNA replication and several essential cellular activities required for rapid bacterial growth, including respiration, central carbon metabolism, fatty acid synthesis, phospholipid synthesis, and protein synthesis. Unexpectedly, the results indicate that multiple regulatory systems are involved in coordinating DNA replication with cell physiology, with some of the regulatory systems targeting oriC while others act in a oriC-independent manner. We propose that distinct regulatory systems are utilized to control DNA replication in response to diverse physiological and chemical changes. PMID:25340815

  19. The Replication Mechanism in a Romanian ERP System Environment

    Directory of Open Access Journals (Sweden)

    Iuliana SCORTA

    2008-01-01

    Full Text Available Modern Relational Database Management Systems have Replication technology. Large enterprises are often spread around the country, and although WANs can be very fast and reliable these days, it is often better for each location to have a local copy of data rather than have a single database at a central location. This usually means that replication is a requirement in order for each location to have the most up-to-date data. This paper reveals a replication mechanism implemented in a Romanian ERP system environment.

  20. Evolution of complexity in RNA-like replicator systems

    Directory of Open Access Journals (Sweden)

    Hogeweg Paulien

    2008-03-01

    Full Text Available Abstract Background The evolution of complexity is among the most important questions in biology. The evolution of complexity is often observed as the increase of genetic information or that of the organizational complexity of a system. It is well recognized that the formation of biological organization – be it of molecules or ecosystems – is ultimately instructed by the genetic information, whereas it is also true that the genetic information is functional only in the context of the organization. Therefore, to obtain a more complete picture of the evolution of complexity, we must study the evolution of both information and organization. Results Here we investigate the evolution of complexity in a simulated RNA-like replicator system. The simplicity of the system allows us to explicitly model the genotype-phenotype-interaction mapping of individual replicators, whereby we avoid preconceiving the functionality of genotypes (information or the ecological organization of replicators in the model. In particular, the model assumes that interactions among replicators – to replicate or to be replicated – depend on their secondary structures and base-pair matching. The results showed that a population of replicators, originally consisting of one genotype, evolves to form a complex ecosystem of up to four species. During this diversification, the species evolve through acquiring unique genotypes with distinct ecological functionality. The analysis of this diversification reveals that parasitic replicators, which have been thought to destabilize the replicator's diversity, actually promote the evolution of diversity through generating a novel "niche" for catalytic replicators. This also makes the current replicator system extremely stable upon the evolution of parasites. The results also show that the stability of the system crucially depends on the spatial pattern formation of replicators. Finally, the evolutionary dynamics is shown to

  1. Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates.

    Science.gov (United States)

    Kurat, Christoph F; Yeeles, Joseph T P; Patel, Harshil; Early, Anne; Diffley, John F X

    2017-01-05

    The integrity of eukaryotic genomes requires rapid and regulated chromatin replication. How this is accomplished is still poorly understood. Using purified yeast replication proteins and fully chromatinized templates, we have reconstituted this process in vitro. We show that chromatin enforces DNA replication origin specificity by preventing non-specific MCM helicase loading. Helicase activation occurs efficiently in the context of chromatin, but subsequent replisome progression requires the histone chaperone FACT (facilitates chromatin transcription). The FACT-associated Nhp6 protein, the nucleosome remodelers INO80 or ISW1A, and the lysine acetyltransferases Gcn5 and Esa1 each contribute separately to maximum DNA synthesis rates. Chromatin promotes the regular priming of lagging-strand DNA synthesis by facilitating DNA polymerase α function at replication forks. Finally, nucleosomes disrupted during replication are efficiently re-assembled into regular arrays on nascent DNA. Our work defines the minimum requirements for chromatin replication in vitro and shows how multiple chromatin factors might modulate replication fork rates in vivo. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  2. SILHIL Replication of Electric Aircraft Powertrain Dynamics and Inner-Loop Control for V&V of System Health Management Routines

    Science.gov (United States)

    Bole, Brian; Teubert, Christopher Allen; Cuong Chi, Quach; Hogge, Edward; Vazquez, Sixto; Goebel, Kai; George, Vachtsevanos

    2013-01-01

    Software-in-the-loop and Hardware-in-the-loop testing of failure prognostics and decision making tools for aircraft systems will facilitate more comprehensive and cost-effective testing than what is practical to conduct with flight tests. A framework is described for the offline recreation of dynamic loads on simulated or physical aircraft powertrain components based on a real-time simulation of airframe dynamics running on a flight simulator, an inner-loop flight control policy executed by either an autopilot routine or a human pilot, and a supervisory fault management control policy. The creation of an offline framework for verifying and validating supervisory failure prognostics and decision making routines is described for the example of battery charge depletion failure scenarios onboard a prototype electric unmanned aerial vehicle.

  3. Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.

    Science.gov (United States)

    Cressey, Tessa; Brauburger, Kristina; Mühlberger, Elke

    2017-01-01

    In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.

  4. Vaccine-Mediated Mechanisms Controlling Replication of Francisella tularensis in Human Peripheral Blood Mononuclear Cells Using a Co-culture System

    Directory of Open Access Journals (Sweden)

    Kjell Eneslätt

    2018-02-01

    Full Text Available Cell-mediated immunity (CMI is normally required for efficient protection against intracellular infections, however, identification of correlates is challenging and they are generally lacking. Francisella tularensis is a highly virulent, facultative intracellular bacterium and CMI is critically required for protection against the pathogen, but how this is effectuated in humans is poorly understood. To understand the protective mechanisms, we established an in vitro co-culture assay to identify how control of infection of F. tularensis is accomplished by human cells and hypothesized that the model will mimic in vivo immune mechanisms. Non-adherent peripheral blood mononuclear cells (PBMCs were expanded with antigen and added to cultures with adherent PBMC infected with the human vaccine strain, LVS, or the highly virulent SCHU S4 strain. Intracellular numbers of F. tularensis was followed for 72 h and secreted and intracellular cytokines were analyzed. Addition of PBMC expanded from naïve individuals, i.e., those with no record of immunization to F. tularensis, generally resulted in little or no control of intracellular bacterial growth, whereas addition of PBMC from a majority of F. tularensis-immune individuals executed static and sometimes cidal effects on intracellular bacteria. Regardless of infecting strain, statistical differences between the two groups were significant, P < 0.05. Secretion of 11 cytokines was analyzed after 72 h of infection and significant differences with regard to secretion of IFN-γ, TNF, and MIP-1β was observed between immune and naïve individuals for LVS-infected cultures. Also, in LVS-infected cultures, CD4 T cells from vaccinees, but not CD8 T cells, showed significantly higher expression of IFN-γ, MIP-1β, TNF, and CD107a than cells from naïve individuals. The co-culture system appears to identify correlates of immunity that are relevant for the understanding of mechanisms of the protective host immunity to

  5. Entanglement replication in driven dissipative many-body systems.

    Science.gov (United States)

    Zippilli, S; Paternostro, M; Adesso, G; Illuminati, F

    2013-01-25

    We study the dissipative dynamics of two independent arrays of many-body systems, locally driven by a common entangled field. We show that in the steady state the entanglement of the driving field is reproduced in an arbitrarily large series of inter-array entangled pairs over all distances. Local nonclassical driving thus realizes a scale-free entanglement replication and long-distance entanglement distribution mechanism that has immediate bearing on the implementation of quantum communication networks.

  6. Stochastic Petri net analysis of a replicated file system

    Science.gov (United States)

    Bechta Dugan, Joanne; Ciardo, Gianfranco

    1989-01-01

    A stochastic Petri-net model of a replicated file system is presented for a distributed environment where replicated files reside on different hosts and a voting algorithm is used to maintain consistency. Witnesses, which simply record the status of the file but contain no data, can be used in addition to or in place of files to reduce overhead. A model sufficiently detailed to include file status (current or out-of-date), as well as failure and repair of hosts where copies or witnesses reside, is presented. The number of copies and witnesses is a parameter of the model. Two different majority protocols are examined, one where a majority of all copies and witnesses is necessary to form a quorum, and the other where only a majority of the copies and witnesses on operational hosts is needed. The latter, known as adaptive voting, is shown to increase file availability in most cases.

  7. Replication Strategy for Spatiotemporal Data Based on Distributed Caching System.

    Science.gov (United States)

    Xiong, Lian; Yang, Liu; Tao, Yang; Xu, Juan; Zhao, Lun

    2018-01-14

    The replica strategy in distributed cache can effectively reduce user access delay and improve system performance. However, developing a replica strategy suitable for varied application scenarios is still quite challenging, owing to differences in user access behavior and preferences. In this paper, a replication strategy for spatiotemporal data (RSSD) based on a distributed caching system is proposed. By taking advantage of the spatiotemporal locality and correlation of user access, RSSD mines high popularity and associated files from historical user access information, and then generates replicas and selects appropriate cache node for placement. Experimental results show that the RSSD algorithm is simple and efficient, and succeeds in significantly reducing user access delay.

  8. Replication Strategy for Spatiotemporal Data Based on Distributed Caching System

    Science.gov (United States)

    Xiong, Lian; Tao, Yang; Xu, Juan; Zhao, Lun

    2018-01-01

    The replica strategy in distributed cache can effectively reduce user access delay and improve system performance. However, developing a replica strategy suitable for varied application scenarios is still quite challenging, owing to differences in user access behavior and preferences. In this paper, a replication strategy for spatiotemporal data (RSSD) based on a distributed caching system is proposed. By taking advantage of the spatiotemporal locality and correlation of user access, RSSD mines high popularity and associated files from historical user access information, and then generates replicas and selects appropriate cache node for placement. Experimental results show that the RSSD algorithm is simple and efficient, and succeeds in significantly reducing user access delay. PMID:29342897

  9. Rolling replication of UV-irradiated duplex DNA in the phi X174 replicative-form----single-strand replication system in vitro

    International Nuclear Information System (INIS)

    Shavitt, O.; Livneh, Z.

    1989-01-01

    Cloning of the phi X174 viral origin of replication into phage M13mp8 produced an M13-phi X174 chimera, the DNA of which directed efficient replicative-form----single-strand rolling replication in vitro. This replication assay was performed with purified phi X174-encoded gene A protein, Escherichia coli rep helicase, single-stranded DNA-binding protein, and DNA polymerase III holoenzyme. The nicking of replicative-form I (RFI) DNA by gene A protein was essentially unaffected by the presence of UV lesions in the DNA. However, unwinding of UV-irradiated DNA by the rep helicase was inhibited twofold as compared with unwinding of the unirradiated substrate. UV irradiation of the substrate DNA caused a strong inhibition in its ability to direct DNA synthesis. However, even DNA preparations that contained as many as 10 photodimers per molecule still supported the synthesis of progeny full-length single-stranded DNA. The appearance of full-length radiolabeled products implied at least two full rounds of replication, since the first round released the unlabeled plus viral strand of the duplex DNA. Pretreatment of the UV-irradiated DNA substrate with purified pyrimidine dimer endonuclease from Micrococcus luteus, which converted photodimer-containing supercoiled RFI DNA into relaxed, nicked RFII DNA and thus prevented its replication, reduced DNA synthesis by 70%. Analysis of radiolabeled replication products by agarose gel electrophoresis followed by autoradiography revealed that this decrease was due to a reduction in the synthesis of progeny full-length single-stranded DNA. This implies that 70 to 80% of the full-length DNA products produced in this system were synthesized on molecules that carried photodimers

  10. Viral hijacking of a replicative helicase loader and its implications for helicase loading control and phage replication

    Energy Technology Data Exchange (ETDEWEB)

    Hood, Iris V.; Berger, James M.

    2016-05-31

    Replisome assembly requires the loading of replicative hexameric helicases onto origins by AAA+ ATPases. How loader activity is appropriately controlled remains unclear. Here, we use structural and biochemical analyses to establish how an antimicrobial phage protein interferes with the function of theStaphylococcus aureusreplicative helicase loader, DnaI. The viral protein binds to the loader’s AAA+ ATPase domain, allowing binding of the host replicative helicase but impeding loader self-assembly and ATPase activity. Close inspection of the complex highlights an unexpected locus for the binding of an interdomain linker element in DnaI/DnaC-family proteins. We find that the inhibitor protein is genetically coupled to a phage-encoded homolog of the bacterial helicase loader, which we show binds to the host helicase but not to the inhibitor itself. These findings establish a new approach by which viruses can hijack host replication processes and explain how loader activity is internally regulated to prevent aberrant auto-association.

  11. Surface topography analysis for dimensional quality control of replication at the micrometre scale

    DEFF Research Database (Denmark)

    Balcon, M.; Marinello, F.; Tosello, Guido

    2011-01-01

    Replication of geometrical features and surfaces are present at different production levels, from realization of moulds to final product. Geometrical features must be reproduced within specification limits, to ensure product functionality . In order to control the replication quality, mould...... and replica surfaces must be quantitatively analysed and compared. In the present work, reference simulated surfaces were considered and studied in order to evaluate the effectiveness and traceability of different analysis tools for replication quality control. Topographies were analysed simulating different...

  12. DNA Copy-Number Control through Inhibition of Replication Fork Progression

    Directory of Open Access Journals (Sweden)

    Jared T. Nordman

    2014-11-01

    Full Text Available Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell-cycle regulation. Importantly, DNA replication is highly influenced by developmental cues. In Drosophila, specific regions of the genome are repressed for DNA replication during differentiation by the SNF2 domain-containing protein SUUR through an unknown mechanism. We demonstrate that SUUR is recruited to active replication forks and mediates the repression of DNA replication by directly inhibiting replication fork progression instead of functioning as a replication fork barrier. Mass spectrometry identification of SUUR-associated proteins identified the replicative helicase member CDC45 as a SUUR-associated protein, supporting a role for SUUR directly at replication forks. Our results reveal that control of eukaryotic DNA copy number can occur through the inhibition of replication fork progression.

  13. Diagonalization of replicated transfer matrices for disordered Ising spin systems

    International Nuclear Information System (INIS)

    Nikoletopoulos, T; Coolen, A C C

    2004-01-01

    We present an alternative procedure for solving the eigenvalue problem of replicated transfer matrices describing disordered spin systems with (random) 1D nearest neighbour bonds and/or random fields, possibly in combination with (random) long range bonds. Our method is based on transforming the original eigenvalue problem for a 2 n x 2 n matrix (where n → 0) into an eigenvalue problem for integral operators. We first develop our formalism for the Ising chain with random bonds and fields, where we recover known results. We then apply our methods to models of spins which interact simultaneously via a one-dimensional ring and via more complex long-range connectivity structures, e.g., (1 + ∞)-dimensional neural networks and 'small-world' magnets. Numerical simulations confirm our predictions satisfactorily

  14. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2.

    Science.gov (United States)

    Mendez-Bermudez, Aaron; Lototska, Liudmyla; Bauwens, Serge; Giraud-Panis, Marie-Josèphe; Croce, Olivier; Jamet, Karine; Irizar, Agurtzane; Mowinckel, Macarena; Koundrioukoff, Stephane; Nottet, Nicolas; Almouzni, Genevieve; Teulade-Fichou, Mare-Paule; Schertzer, Michael; Perderiset, Mylène; Londoño-Vallejo, Arturo; Debatisse, Michelle; Gilson, Eric; Ye, Jing

    2018-05-03

    Hard-to-replicate regions of chromosomes (e.g., pericentromeres, centromeres, and telomeres) impede replication fork progression, eventually leading, in the event of replication stress, to chromosome fragility, aging, and cancer. Our knowledge of the mechanisms controlling the stability of these regions is essentially limited to telomeres, where fragility is counteracted by the shelterin proteins. Here we show that the shelterin subunit TRF2 ensures progression of the replication fork through pericentromeric heterochromatin, but not centromeric chromatin. In a process involving its N-terminal basic domain, TRF2 binds to pericentromeric Satellite III sequences during S phase, allowing the recruitment of the G-quadruplex-resolving helicase RTEL1 to facilitate fork progression. We also show that TRF2 is required for the stability of other heterochromatic regions localized throughout the genome, paving the way for future research on heterochromatic replication and its relationship with aging and cancer. Copyright © 2018 Elsevier Inc. All rights reserved.

  15. Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

    Science.gov (United States)

    Fujiwara, Kei; Katayama, Tsutomu; Nomura, Shin-ichiro M.

    2013-01-01

    Replication of all living cells relies on the multirounds flow of the central dogma. Especially, expression of DNA replication proteins is a key step to circulate the processes of the central dogma. Here we achieved the entire sequential transcription–translation–replication process by autonomous expression of chromosomal DNA replication machineries from a reconstituted transcription–translation system (PURE system). We found that low temperature is essential to express a complex protein, DNA polymerase III, in a single tube using the PURE system. Addition of the 13 genes, encoding initiator, DNA helicase, helicase loader, RNA primase and DNA polymerase III to the PURE system gave rise to a DNA replication system by a coupling manner. An artificial genetic circuit demonstrated that the DNA produced as a result of the replication is able to provide genetic information for proteins, indicating the in vitro central dogma can sequentially undergo two rounds. PMID:23737447

  16. Report on best practice for micro/nano replication process data acquisition systems

    DEFF Research Database (Denmark)

    Islam, Mohammad Aminul

    The main objective of this deliverable is to collect detailed description on the best practice for micro/ nano replication process data acquisition system. In particular the following aspects are summed up in the deliverable: Process requirements for COTECH demonstrators General description of th....... This report presents the important processes and process parameters required for the realization of COTECH demonstrator. It also presents the cutting edge sensor systems used for different process and set a guideline to integrate sensors in COTECH tools.......The main objective of this deliverable is to collect detailed description on the best practice for micro/ nano replication process data acquisition system. In particular the following aspects are summed up in the deliverable: Process requirements for COTECH demonstrators General description...... of the processes and process parameters State-of-the-art process control and data acquisition systems Sensor integration in COTECH tools Expected COTECH improvement in process control and data acquisition system Information provide by this deliverable will be used as input to the COTECH process control system...

  17. DEVELOPMENT OF TRANSPORT SUBSYSTEM STREAMING DATA REPLICATION CLUSTER IN CORBA-SYSTEM WITH ZEROMQ TECHNOLOGY

    Directory of Open Access Journals (Sweden)

    F. A. Kozlov

    2013-03-01

    Full Text Available The article deals with the peculiarities of distributed cluster system creation with streaming data replication. Ways of replication cluster implementation in CORBA-systems with ZeroMq technology are presented. Major advantages of ZeroMQ technology over similar technologies are considered in this type distributed systems creation.

  18. Understanding how replication processes can maintain systems away from equilibrium using Algorithmic Information Theory.

    Science.gov (United States)

    Devine, Sean D

    2016-02-01

    Replication can be envisaged as a computational process that is able to generate and maintain order far-from-equilibrium. Replication processes, can self-regulate, as the drive to replicate can counter degradation processes that impact on a system. The capability of replicated structures to access high quality energy and eject disorder allows Landauer's principle, in conjunction with Algorithmic Information Theory, to quantify the entropy requirements to maintain a system far-from-equilibrium. Using Landauer's principle, where destabilising processes, operating under the second law of thermodynamics, change the information content or the algorithmic entropy of a system by ΔH bits, replication processes can access order, eject disorder, and counter the change without outside interventions. Both diversity in replicated structures, and the coupling of different replicated systems, increase the ability of the system (or systems) to self-regulate in a changing environment as adaptation processes select those structures that use resources more efficiently. At the level of the structure, as selection processes minimise the information loss, the irreversibility is minimised. While each structure that emerges can be said to be more entropically efficient, as such replicating structures proliferate, the dissipation of the system as a whole is higher than would be the case for inert or simpler structures. While a detailed application to most real systems would be difficult, the approach may well be useful in understanding incremental changes to real systems and provide broad descriptions of system behaviour. Copyright © 2016 The Author. Published by Elsevier Ireland Ltd.. All rights reserved.

  19. Controlled initiation of chromosomal replication in Escherichia coli requires functional Hda protein.

    Science.gov (United States)

    Camara, Johanna Eltz; Skarstad, Kirsten; Crooke, Elliott

    2003-05-01

    Regulatory inactivation of DnaA helps ensure that the Escherichia coli chromosome is replicated only once per cell cycle, through accelerated hydrolysis of active replication initiator ATP-DnaA to inactive ADP-DnaA. Analysis of deltahda strains revealed that the regulatory inactivation of DnaA component Hda is necessary for maintaining controlled initiation but not for cell growth or viability.

  20. Biological evolution of replicator systems: towards a quantitative approach.

    Science.gov (United States)

    Martin, Osmel; Horvath, J E

    2013-04-01

    The aim of this work is to study the features of a simple replicator chemical model of the relation between kinetic stability and entropy production under the action of external perturbations. We quantitatively explore the different paths leading to evolution in a toy model where two independent replicators compete for the same substrate. To do that, the same scenario described originally by Pross (J Phys Org Chem 17:312-316, 2004) is revised and new criteria to define the kinetic stability are proposed. Our results suggest that fast replicator populations are continually favored by the effects of strong stochastic environmental fluctuations capable to determine the global population, the former assumed to be the only acting evolution force. We demonstrate that the process is continually driven by strong perturbations only, and that population crashes may be useful proxies for these catastrophic environmental fluctuations. As expected, such behavior is particularly enhanced under very large scale perturbations, suggesting a likely dynamical footprint in the recovery patterns of new species after mass extinction events in the Earth's geological past. Furthermore, the hypothesis that natural selection always favors the faster processes may give theoretical support to different studies that claim the applicability of maximum principles like the Maximum Metabolic Flux (MMF) or Maximum Entropy Productions Principle (MEPP), seen as the main goal of biological evolution.

  1. Microscreen effects on water quality in replicated recirculating aquaculture systems

    DEFF Research Database (Denmark)

    Fernandes, Paulo; Pedersen, Lars-Flemming; Pedersen, Per Bovbjerg

    2015-01-01

    This study investigated the effects of three microscreen mesh sizes (100, 60 and 20 μm) on water quality and rainbow trout (Oncorhynchus mykiss) performance compared to a control group without microscreens, in triplicated recirculating aquaculture systems (RAS). Operational conditions were kept....... Fish performed similarly in all treatments. Preliminary screening of trout gills did not reveal any pathological changes related to microscreen filtration and the resulting water quality. Biofilter performance was also unaffected, with 0′-order nitrification rates (k0a) being equivalent for all twelve...

  2. Ecology and Evolution in the RNA World Dynamics and Stability of Prebiotic Replicator Systems

    Science.gov (United States)

    Szilágyi, András; Kun, Ádám; Könnyű, Balázs; Czárán, Tamás

    2017-01-01

    As of today, the most credible scientific paradigm pertaining to the origin of life on Earth is undoubtedly the RNA World scenario. It is built on the assumption that catalytically active replicators (most probably RNA-like macromolecules) may have been responsible for booting up life almost four billion years ago. The many different incarnations of nucleotide sequence (string) replicator models proposed recently are all attempts to explain on this basis how the genetic information transfer and the functional diversity of prebiotic replicator systems may have emerged, persisted and evolved into the first living cell. We have postulated three necessary conditions for an RNA World model system to be a dynamically feasible representation of prebiotic chemical evolution: (1) it must maintain and transfer a sufficient diversity of information reliably and indefinitely, (2) it must be ecologically stable and (3) it must be evolutionarily stable. In this review, we discuss the best-known prebiotic scenarios and the corresponding models of string-replicator dynamics and assess them against these criteria. We suggest that the most popular of prebiotic replicator systems, the hypercycle, is probably the worst performer in almost all of these respects, whereas a few other model concepts (parabolic replicator, open chaotic flows, stochastic corrector, metabolically coupled replicator system) are promising candidates for development into coherent models that may become experimentally accessible in the future. PMID:29186916

  3. RIPK1 and PGAM5 Control Leishmania Replication through Distinct Mechanisms.

    Science.gov (United States)

    Farias Luz, Nivea; Balaji, Sakthi; Okuda, Kendi; Barreto, Aline Silva; Bertin, John; Gough, Peter J; Gazzinelli, Ricardo; Almeida, Roque P; Bozza, Marcelo T; Borges, Valeria M; Chan, Francis Ka-Ming

    2016-06-15

    Leishmaniasis is an important parasitic disease found in the tropics and subtropics. Cutaneous and visceral leishmaniasis affect an estimated 1.5 million people worldwide. Despite its human health relevance, relatively little is known about the cell death pathways that control Leishmania replication in the host. Necroptosis is a recently identified form of cell death with potent antiviral effects. Receptor interacting protein kinase 1 (RIPK1) is a critical kinase that mediates necroptosis downstream of death receptors and TLRs. Heme, a product of hemoglobin catabolism during certain intracellular pathogen infections, is also a potent inducer of macrophage necroptosis. We found that human visceral leishmaniasis patients exhibit elevated serum levels of heme. Therefore, we examined the impact of heme and necroptosis on Leishmania replication. Indeed, heme potently inhibited Leishmania replication in bone marrow-derived macrophages. Moreover, we found that inhibition of RIPK1 kinase activity also enhanced parasite replication in the absence of heme. We further found that the mitochondrial phosphatase phosphoglycerate mutase family member 5 (PGAM5), a putative downstream effector of RIPK1, was also required for inhibition of Leishmania replication. In mouse infection, both PGAM5 and RIPK1 kinase activity are required for IL-1β expression in response to Leishmania However, PGAM5, but not RIPK1 kinase activity, was directly responsible for Leishmania-induced IL-1β secretion and NO production in bone marrow-derived macrophages. Collectively, these results revealed that RIPK1 and PGAM5 function independently to exert optimal control of Leishmania replication in the host. Copyright © 2016 by The American Association of Immunologists, Inc.

  4. Ecology and evolution in the RNA world dynamics and stability of prebiotic replicator systems

    DEFF Research Database (Denmark)

    Szilágyi, András; Zachar, István; Scheuring, István

    2017-01-01

    billion years ago. The many different incarnations of nucleotide sequence (string) replicator models proposed recently are all attempts to explain on this basis how the genetic information transfer and the functional diversity of prebiotic replicator systems may have emerged, persisted and evolved...... into the first living cell. We have postulated three necessary conditions for an RNA World model system to be a dynamically feasible representation of prebiotic chemical evolution: (1) it must maintain and transfer a sufficient diversity of information reliably and indefinitely, (2) it must be ecologically...... stable and (3) it must be evolutionarily stable. In this review, we discuss the best-known prebiotic scenarios and the corresponding models of string-replicator dynamics and assess them against these criteria. We suggest that the most popular of prebiotic replicator systems, the hypercycle, is probably...

  5. Sustainability of a Compartmentalized Host-Parasite Replicator System under Periodic Washout-Mixing Cycles

    Directory of Open Access Journals (Sweden)

    Taro Furubayashi

    2018-01-01

    Full Text Available The emergence and dominance of parasitic replicators are among the major hurdles for the proliferation of primitive replicators. Compartmentalization of replicators is proposed to relieve the parasite dominance; however, it remains unclear under what conditions simple compartmentalization uncoupled with internal reaction secures the long-term survival of a population of primitive replicators against incessant parasite emergence. Here, we investigate the sustainability of a compartmentalized host-parasite replicator (CHPR system undergoing periodic washout-mixing cycles, by constructing a mathematical model and performing extensive simulations. We describe sustainable landscapes of the CHPR system in the parameter space and elucidate the mechanism of phase transitions between sustainable and extinct regions. Our findings revealed that a large population size of compartments, a high mixing intensity, and a modest amount of nutrients are important factors for the robust survival of replicators. We also found two distinctive sustainable phases with different mixing intensities. These results suggest that a population of simple host–parasite replicators assumed before the origin of life can be sustained by a simple compartmentalization with periodic washout-mixing processes.

  6. Improvement of replication fidelity in injection moulding of nano structures using an induction heating system

    DEFF Research Database (Denmark)

    Menotti, Stefano; Hansen, Hans Nørgaard; Bissacco, Giuliano

    2014-01-01

    In today’s industry, applications involving surface pattering with sub-μm scale structures have shown a high interest. The replication of these structures by injection molding leads to special requirements for the mold in order to ensure proper replication and an acceptable cycle time. A tool ins...... quantitatively characterized by atomic force microscopy comparing the measurement in the nickel insert with the corresponding polymer nano-features. The experimental results show that the use of the induction heating system is an efficient way to improve the pattern replication....

  7. The ATLAS Detector Control System

    International Nuclear Information System (INIS)

    Lantzsch, K; Braun, H; Hirschbuehl, D; Kersten, S; Arfaoui, S; Franz, S; Gutzwiller, O; Schlenker, S; Tsarouchas, C A; Mindur, B; Hartert, J; Zimmermann, S; Talyshev, A; Oliveira Damazio, D; Poblaguev, A; Martin, T; Thompson, P D; Caforio, D; Sbarra, C; Hoffmann, D

    2012-01-01

    The ATLAS experiment is one of the multi-purpose experiments at the Large Hadron Collider (LHC) at CERN, constructed to study elementary particle interactions in collisions of high-energy proton beams. Twelve different sub detectors as well as the common experimental infrastructure are controlled and monitored by the Detector Control System (DCS) using a highly distributed system of 140 server machines running the industrial SCADA product PVSS. Higher level control system layers allow for automatic control procedures, efficient error recognition and handling, manage the communication with external systems such as the LHC controls, and provide a synchronization mechanism with the ATLAS data acquisition system. Different databases are used to store the online parameters of the experiment, replicate a subset used for physics reconstruction, and store the configuration parameters of the systems. This contribution describes the computing architecture and software tools to handle this complex and highly interconnected control system.

  8. The ATLAS Detector Control System

    Science.gov (United States)

    Lantzsch, K.; Arfaoui, S.; Franz, S.; Gutzwiller, O.; Schlenker, S.; Tsarouchas, C. A.; Mindur, B.; Hartert, J.; Zimmermann, S.; Talyshev, A.; Oliveira Damazio, D.; Poblaguev, A.; Braun, H.; Hirschbuehl, D.; Kersten, S.; Martin, T.; Thompson, P. D.; Caforio, D.; Sbarra, C.; Hoffmann, D.; Nemecek, S.; Robichaud-Veronneau, A.; Wynne, B.; Banas, E.; Hajduk, Z.; Olszowska, J.; Stanecka, E.; Bindi, M.; Polini, A.; Deliyergiyev, M.; Mandic, I.; Ertel, E.; Marques Vinagre, F.; Ribeiro, G.; Santos, H. F.; Barillari, T.; Habring, J.; Huber, J.; Arabidze, G.; Boterenbrood, H.; Hart, R.; Iakovidis, G.; Karakostas, K.; Leontsinis, S.; Mountricha, E.; Ntekas, K.; Filimonov, V.; Khomutnikov, V.; Kovalenko, S.; Grassi, V.; Mitrevski, J.; Phillips, P.; Chekulaev, S.; D'Auria, S.; Nagai, K.; Tartarelli, G. F.; Aielli, G.; Marchese, F.; Lafarguette, P.; Brenner, R.

    2012-12-01

    The ATLAS experiment is one of the multi-purpose experiments at the Large Hadron Collider (LHC) at CERN, constructed to study elementary particle interactions in collisions of high-energy proton beams. Twelve different sub detectors as well as the common experimental infrastructure are controlled and monitored by the Detector Control System (DCS) using a highly distributed system of 140 server machines running the industrial SCADA product PVSS. Higher level control system layers allow for automatic control procedures, efficient error recognition and handling, manage the communication with external systems such as the LHC controls, and provide a synchronization mechanism with the ATLAS data acquisition system. Different databases are used to store the online parameters of the experiment, replicate a subset used for physics reconstruction, and store the configuration parameters of the systems. This contribution describes the computing architecture and software tools to handle this complex and highly interconnected control system.

  9. Low cost management of replicated data in fault-tolerant distributed systems

    Science.gov (United States)

    Joseph, Thomas A.; Birman, Kenneth P.

    1990-01-01

    Many distributed systems replicate data for fault tolerance or availability. In such systems, a logical update on a data item results in a physical update on a number of copies. The synchronization and communication required to keep the copies of replicated data consistent introduce a delay when operations are performed. A technique is described that relaxes the usual degree of synchronization, permitting replicated data items to be updated concurrently with other operations, while at the same time ensuring that correctness is not violated. The additional concurrency thus obtained results in better response time when performing operations on replicated data. How this technique performs in conjunction with a roll-back and a roll-forward failure recovery mechanism is also discussed.

  10. Control of HIV-1 in Elite Suppressors despite Ongoing Replication and Evolution in Plasma Virus▿

    Science.gov (United States)

    O'Connell, Karen A.; Brennan, Timothy P.; Bailey, Justin R.; Ray, Stuart C.; Siliciano, Robert F.; Blankson, Joel N.

    2010-01-01

    A subset of HIV-1-infected patients known as elite controllers or suppressors (ES) control the virus naturally. We have previously demonstrated sequence discordance between proviral and plasma gag clones in ES, much of which can be attributed to selective pressure from the host (J. R. Bailey, T. M. Williams, R. F. Siliciano, and J. N. Blankson, J. Exp. Med. 203:1357-1369, 2006). However, it is not clear whether ongoing viral replication continues in ES once the control of viremia has been established or whether selective pressure impacts this evolution. The cytotoxic T-lymphocyte (CTL) response in ES often targets Gag and frequently is superior to that of HIV-1 progressors, partially due to the HLA class I alleles B*57/5801 and B*27, which are overrepresented in ES. We therefore examined longitudinal plasma and proviral gag sequences from HLA-B*57/5801 and -B*27 ES. Despite the highly conserved nature of gag, we observed clear evidence of evolution in the plasma virus, largely due to synonymous substitutions. In contrast, evolution was rare in proviral clones, suggesting that ongoing replication in ES does not permit the significant reseeding of the latent reservoir. Interestingly, there was little continual evolution in CTL epitopes, and we detected de novo CTL responses to autologous viral mutants. Thus, some ES control viremia despite ongoing replication and evolution. PMID:20444904

  11. Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae.

    Science.gov (United States)

    Maglennon, Gareth A; Cook, Beth S; Matthews, Dominic; Deeney, Alannah S; Bossé, Janine T; Langford, Paul R; Maskell, Duncan J; Tucker, Alexander W; Wren, Brendan W; Rycroft, Andrew N

    2013-07-29

    Mycoplasma hyopneumoniae is a prevalent swine respiratory pathogen that is a major cause of economic loss to pig producers. Control is achieved by a combination of antimicrobials, vaccination and management practices, but current vaccines offer only partial control and there is a need for improved preventative strategies. A major barrier to advances in understanding the pathogenesis of M. hyopneumoniae and in developing new vaccines is the lack of tools to genetically manipulate the organism. We describe the development and optimisation of the first successful plasmid-based system for the genetic manipulation of M. hyopneumoniae. Our artificial plasmids contain the origin of replication (oriC) of M. hyopneumoniae along with tetM, conferring resistance to tetracycline. With these plasmids, we have successfully transformed M. hyopneumoniae strain 232 by electroporation, generating tetracycline resistant organisms. The persistence of extrachromosomal plasmid and maintenance of plasmid DNA over serial passages shows that these artificial plasmids are capable of self-replication in M. hyopneumoniae. In addition to demonstrating the amenability of M. hyopneumoniae to genetic manipulation and in optimising the conditions necessary for successful transformation, we have used this system to determine the minimum functional oriC of M. hyopneumoniae. In doing so, we have developed a plasmid with a small oriC that is stably maintained over multiple passages that may be useful in generating targeted gene disruptions. In conclusion, we have generated a set of plasmids that will be valuable in studies of M. hyopneumoniae pathogenesis and provide a major step forward in the study of this important swine pathogen.

  12. Category of Metabolic-Replication Systems in Biology and Medicine

    OpenAIRE

    I. C. Baianu

    2012-01-01

    Metabolic-repair models, or (M,R)-systems were introduced in Relational Biology by Robert Rosen. Subsequently, Rosen represented such (M,R)-systems (or simply MRs)in terms of categories of sets, deliberately selected without any structure other than the discrete topology of sets. Theoreticians of life's origins postulated that Life on Earth has begun with the simplest possible organism, called the primordial. Mathematicians interested in biology attempted to answer this important questio...

  13. Database Replication

    CERN Document Server

    Kemme, Bettina

    2010-01-01

    Database replication is widely used for fault-tolerance, scalability and performance. The failure of one database replica does not stop the system from working as available replicas can take over the tasks of the failed replica. Scalability can be achieved by distributing the load across all replicas, and adding new replicas should the load increase. Finally, database replication can provide fast local access, even if clients are geographically distributed clients, if data copies are located close to clients. Despite its advantages, replication is not a straightforward technique to apply, and

  14. Replication quality control of metal and polymer micro structured optical surfaces

    DEFF Research Database (Denmark)

    Gasparin, Stefania; Tosello, Guido; Hansen, Hans Nørgaard

    2011-01-01

    replication methods based on polymer casting. The replica method is used in order to avoid damages of the structures and make feasible the measurement of optical specimens with non-contact instruments. Results show a quality replication equal to 95 - 99%. In both investigations the uncertainty......Non contact measurements are preferred for the characterization of ultra-finely finished surfaces which is particularly challenging if damages of the structures should be avoided. However, it is not always possible to use these methods because low roughness in metallic materials, as optical...... surfaces, quite often results in mirror-like surfaces which scatter the light and invalidate the optical measurements. This paper focuses on an analysis of a micro-structured optical component and the corresponding mould. A first investigation leads to a control of the manufacturing process through...

  15. Chelonia: A self-healing, replicated storage system

    International Nuclear Information System (INIS)

    Kerr Nilsen, Jon; Read, Alex; Toor, Salman; Nagy, Zsombor

    2011-01-01

    Chelonia is a novel grid storage system designed to fill the requirements gap between those of large, sophisticated scientific collaborations which have adopted the grid paradigm for their distributed storage needs, and of corporate business communities gravitating towards the cloud paradigm. Chelonia is an integrated system of heterogeneous, geographically dispersed storage sites which is easily and dynamically expandable and optimized for high availability and scalability. The architecture and implementation in term of web-services running inside the Advanced Resource Connector Hosting Environment Dameon (ARC HED) are described and results of tests in both local -area and wide-area networks that demonstrate the fault tolerance, stability and scalability of Chelonia will be presented. In addition, example setups for production deployments for small and medium-sized VO's are described.

  16. Chelonia: A self-healing, replicated storage system

    Science.gov (United States)

    Kerr Nilsen, Jon; Toor, Salman; Nagy, Zsombor; Read, Alex

    2011-12-01

    Chelonia is a novel grid storage system designed to fill the requirements gap between those of large, sophisticated scientific collaborations which have adopted the grid paradigm for their distributed storage needs, and of corporate business communities gravitating towards the cloud paradigm. Chelonia is an integrated system of heterogeneous, geographically dispersed storage sites which is easily and dynamically expandable and optimized for high availability and scalability. The architecture and implementation in term of web-services running inside the Advanced Resource Connector Hosting Environment Dameon (ARC HED) are described and results of tests in both local -area and wide-area networks that demonstrate the fault tolerance, stability and scalability of Chelonia will be presented. In addition, example setups for production deployments for small and medium-sized VO's are described.

  17. Suppression of HIV replication by CD8+regulatory T-cells in elite controllers

    Directory of Open Access Journals (Sweden)

    Wei eLu

    2016-04-01

    Full Text Available We previously demonstrated in the Chinese macaque model that an oral vaccine made of inactivated SIV and lactobacillus plantarum induced CD8+regulatory T-cells which suppressed the activation of SIV+CD4+T-cells, prevented SIV replication and protected macaques from SIV challenges.Here ,we sought whether a similar population of CD8+T-regs would induce the suppression of HIV replication in elite controllers (ECs, a small population (3‰ of HIV-infected patients with undetectable HIV replication. For that purpose, we investigated the in vitro antiviral activity of fresh CD8+T-cells on HIV-infected CD4+T-cells taken from 10 ECs. The 10 ECs had a classical genomic profile: all of them carried the KIR3DL1 gene and nine carried at least one allele of HLA-B:Bw4-80Ile ( i.e. with an isoleucine residue at position 80. In the nine HLA-B:Bw4-80Ile positive patients, we demonstrated a strong viral suppression byKIR3DL1-expressing CD8+T-cells that required cell-to-cell contact to switch off the activation signals in infected CD4+T-cells. KIR3DL1-expressing CD8+T-cells withdrawal and KIR3DL1 neutralization by a specific anti-KIR antibody inhibited the suppression of viral replication. Our findings provide the first evidence for an instrumental role of KIR-expressing CD8+ regulatory T- cells in the natural control of HIV-1 infection.

  18. P1 plasmid replication: initiator sequestration is inadequate to explain control by initiator-binding sites.

    OpenAIRE

    Pal, S K; Chattoraj, D K

    1988-01-01

    The unit-copy plasmid replicon mini-P1 consists of an origin, a gene for an initiator protein, RepA, and a control locus, incA. Both the origin and the incA locus contain repeat sequences that bind RepA. It has been proposed that the incA repeats control replication by sequestering the rate-limiting RepA initiator protein. Here we show that when the concentration of RepA was increased about fourfold beyond its normal physiological level from an inducible source in trans, the copy number of a ...

  19. A Systems Approach to Effectiveness in Catholic Elementary Schools: A Replication and Extension

    Science.gov (United States)

    Mitchell, Roxanne M.; Tarter, C. John

    2011-01-01

    This study replicated an earlier study conducted by Tarter and Hoy (2004) in which an open systems model was used to test a series of hypotheses that explained elements of school performance. Four internal system elements (structure, individual, culture, and politics) of the school were used to explain two sets of school outcomes (student…

  20. An application of the Caputo-Fabrizio operator to replicator-mutator dynamics: Bifurcation, chaotic limit cycles and control

    Science.gov (United States)

    Doungmo Goufo, Emile Franc

    2018-02-01

    The physical behaviors of replicator-mutator processes found in theoretical biophysics, physical chemistry, biochemistry and population biology remain complex with unlimited expressibility. People languages, for instance, have impressively and unpredictably changed over the time in human history. This is mainly due to the collection of small changes and collaboration with other languages. In this paper, the Caputo-Fabrizio operator is applied to a replicator-mutator dynamic taking place in midsts with movement. The model is fully analyzed and solved numerically via the Crank-Nicolson scheme. Stability and convergence results are provided. A concrete application to replicator-mutator dynamics for a population with three strategies is performed with numerical simulations provided for some fixed values of the physical position of the population symbolized by r and the grid points. Physically, it happens that limit cycles appear, not only in function of the mutation parameter μ but also in function of the values given to r . The amplitudes of limit cycles also appear to be proportional to r but the stability of the system remains unaffected. However, those limit cycles instead of disappearing as expected, are immediately followed by chaotic and unpredictable behaviors certainly due to the non-singular kernel used in the model and suitable to non-linear dynamics. Hence, the appearance and disappearance of limit cycles might be controlled by the position variable r which can also apprehend chaos.

  1. Spatiotemporal chaos of self-replicating spots in reaction-diffusion systems.

    Science.gov (United States)

    Wang, Hongli; Ouyang, Qi

    2007-11-23

    The statistical properties of self-replicating spots in the reaction-diffusion Gray-Scott model are analyzed. In the chaotic regime of the system, the spots that dominate the spatiotemporal chaos grow and divide in two or decay into the background randomly and continuously. The rates at which the spots are created and decay are observed to be linearly dependent on the number of spots in the system. We derive a probabilistic description of the spot dynamics based on the statistical independence of spots and thus propose a characterization of the spatiotemporal chaos dominated by replicating spots.

  2. A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication

    Directory of Open Access Journals (Sweden)

    Mary E. Piper

    2010-03-01

    Full Text Available Rift Valley fever virus (RVFV is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly to a wide array of known and previously unknown chemical inhibitors. This system should be useful for screening for small molecule inhibitors of RVFV replication.

  3. A novel system for identification of inhibitors of rift valley Fever virus replication.

    Science.gov (United States)

    Piper, Mary E; Gerrard, Sonja R

    2010-03-01

    Rift Valley fever virus (RVFV) is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs) based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly to a wide array of known and previously unknown chemical inhibitors. This system should be useful for screening for small molecule inhibitors of RVFV replication.

  4. Coupled replicator equations for the dynamics of learning in multiagent systems

    Science.gov (United States)

    Sato, Yuzuru; Crutchfield, James P.

    2003-01-01

    Starting with a group of reinforcement-learning agents we derive coupled replicator equations that describe the dynamics of collective learning in multiagent systems. We show that, although agents model their environment in a self-interested way without sharing knowledge, a game dynamics emerges naturally through environment-mediated interactions. An application to rock-scissors-paper game interactions shows that the collective learning dynamics exhibits a diversity of competitive and cooperative behaviors. These include quasiperiodicity, stable limit cycles, intermittency, and deterministic chaos—behaviors that should be expected in heterogeneous multiagent systems described by the general replicator equations we derive.

  5. Cellular respiration: replicating in vivo systems biology for in ...

    Science.gov (United States)

    This editorial develops a philosophy for expanding the scope of Journal of Breath Research (JBR) into the realm of cellular level study, and links certain topics back to more traditional systemic research for understanding human health based on exhaled breath constituents. The express purpose is to provide a publication outlet for novel breath related research that includes in vitro studies, especially those that explore the biological origin and expression of compounds that may ultimately influence the constituents of exhaled breath. The new topics include all manner of methods and instrumentations for making in vivo and in vitro measurements, the use of different biological media (blood, urine saliva, swabs) including human and microbial cell-lines, in vitro kinetic studies of metabolism, and advances in ex vivo methods for maintaining metabolic competency and viability of biological samples. Traditionally, JBR has published articles on human breath analysis for diagnosing disease, tracking health state, assessing the dose and effect of exogenous chemicals, and contributions of malodorous compounds from the oral/nasal cavity. These have also included research describing novel sampling and analytical technologies, most notably those implementing mass spectrometry, chemical sensors and optical measurement instrumentation (Amann and Smith 2013). The journal’s original scope has also embraced animal models as surrogates for human sampling, new mathematical and

  6. Synthesis of Fault-Tolerant Embedded Systems with Checkpointing and Replication

    DEFF Research Database (Denmark)

    Izosimov, Viacheslav; Pop, Paul; Eles, Petru

    2006-01-01

    We present an approach to the synthesis of fault-tolerant hard real-time systems for safety-critical applications. We use checkpointing with rollback recovery and active replication for tolerating transient faults. Processes are statically scheduled and communications are performed using the time...

  7. Novelty vs. Replicability: Virtues and Vices in the Reward System of Science

    OpenAIRE

    Romero Toro, Felipe

    2017-01-01

    The reward system of science is the priority rule (Merton, 1957). The first scientist making a new discovery is rewarded with prestige while second runners get little or nothing. Strevens (2003, 2011), following Kitcher (1990), defends this reward system arguing that it incentivizes an efficient division of cognitive labor. I argue that this assessment depends on strong implicit assumptions about the replicability of findings. I question these assumptions based on meta-scientific evidence and...

  8. Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex.

    Science.gov (United States)

    Hiraga, Shin-Ichiro; Alvino, Gina M; Chang, Fujung; Lian, Hui-Yong; Sridhar, Akila; Kubota, Takashi; Brewer, Bonita J; Weinreich, Michael; Raghuraman, M K; Donaldson, Anne D

    2014-02-15

    Initiation of eukaryotic DNA replication requires phosphorylation of the MCM complex by Dbf4-dependent kinase (DDK), composed of Cdc7 kinase and its activator, Dbf4. We report here that budding yeast Rif1 (Rap1-interacting factor 1) controls DNA replication genome-wide and describe how Rif1 opposes DDK function by directing Protein Phosphatase 1 (PP1)-mediated dephosphorylation of the MCM complex. Deleting RIF1 partially compensates for the limited DDK activity in a cdc7-1 mutant strain by allowing increased, premature phosphorylation of Mcm4. PP1 interaction motifs within the Rif1 N-terminal domain are critical for its repressive effect on replication. We confirm that Rif1 interacts with PP1 and that PP1 prevents premature Mcm4 phosphorylation. Remarkably, our results suggest that replication repression by Rif1 is itself also DDK-regulated through phosphorylation near the PP1-interacting motifs. Based on our findings, we propose that Rif1 is a novel PP1 substrate targeting subunit that counteracts DDK-mediated phosphorylation during replication. Fission yeast and mammalian Rif1 proteins have also been implicated in regulating DNA replication. Since PP1 interaction sites are evolutionarily conserved within the Rif1 sequence, it is likely that replication control by Rif1 through PP1 is a conserved mechanism.

  9. Semiotic systems with duality of patterning and the issue of cultural replicators.

    Science.gov (United States)

    Schaden, Gerhard; Patin, Cédric

    2017-11-14

    Two major works in recent evolutionary biology have in different ways touched upon the issue of cultural replicators in language, namely Dawkins' Selfish Gene and Maynard Smith and Szathmáry's Major Transitions in Evolution. In the latter, the emergence of language is referred to as the last major transition in evolution (for the time being), a claim we argue to be derived from a crucial property of language, called Duality of Patterning. Prima facie, this property makes natural language look like a structural equivalent to DNA, and its peer in terms of expressive power. We will argue that, if one takes seriously Maynard Smith and Szathmáry's outlook and examines what has been proposed as linguistic replicators, amongst others phonemes and words, the analogy meme-gene becomes problematic. A key issue is the fact that genes and memes are assumed to carry and transmit information, while what has been described as the best candidate for replicatorhood in language, i.e. the phoneme, does by definition not carry meaning. We will argue that semiotic systems with Duality of Pattering (like natural languages) force us to reconsider either the analogy between replicators in the biological and the cultural domain, or what it is to be a replicator in linguistics.

  10. Prelife catalysts and replicators

    OpenAIRE

    Ohtsuki, Hisashi; Nowak, Martin A.

    2009-01-01

    Life is based on replication and evolution. But replication cannot be taken for granted. We must ask what there was prior to replication and evolution. How does evolution begin? We have proposed prelife as a generative system that produces information and diversity in the absence of replication. We model prelife as a binary soup of active monomers that form random polymers. ‘Prevolutionary’ dynamics can have mutation and selection prior to replication. Some sequences might have catalytic acti...

  11. Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere Sequestration.

    Science.gov (United States)

    Hafner, Lukas; Lezaja, Aleksandra; Zhang, Xu; Lemmens, Laure; Shyian, Maksym; Albert, Benjamin; Follonier, Cindy; Nunes, Jose Manuel; Lopes, Massimo; Shore, David; Mattarocci, Stefano

    2018-04-24

    The Saccharomyces cerevisiae telomere-binding protein Rif1 plays an evolutionarily conserved role in control of DNA replication timing by promoting PP1-dependent dephosphorylation of replication initiation factors. However, ScRif1 binding outside of telomeres has never been detected, and it has thus been unclear whether Rif1 acts directly on the replication origins that it controls. Here, we show that, in unperturbed yeast cells, Rif1 primarily regulates late-replicating origins within 100 kb of a telomere. Using the chromatin endogenous cleavage ChEC-seq technique, we robustly detect Rif1 at late-replicating origins that we show are targets of its inhibitory action. Interestingly, abrogation of Rif1 telomere association by mutation of its Rap1-binding module increases Rif1 binding and origin inhibition elsewhere in the genome. Our results indicate that Rif1 inhibits replication initiation by interacting directly with origins and suggest that Rap1-dependent sequestration of Rif1 increases its effective concentration near telomeres, while limiting its action at chromosome-internal sites. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

  12. Replication of simulated prebiotic amphiphile vesicles controlled by experimental lipid physicochemical properties

    International Nuclear Information System (INIS)

    Armstrong, Don L; Zidovetzki, Raphael; Markovitch, Omer; Lancet, Doron

    2011-01-01

    We present a new embodiment of the graded autocatalysis replication domain (GARD) for the growth, replication and evolution of lipid vesicles based on a semi-empirical foundation using experimentally measured kinetic values of selected extant lipid species. Extensive simulations using this formalism elucidated the details of the dependence of the replication and properties of the vesicles on the physicochemical properties and concentrations of the lipids, both in the environment and in the vesicle. As expected, the overall concentration and number of amphiphilic components strongly affect average replication time. Furthermore, variations in acyl chain length and unsaturation of vesicles also influence replication rate, as do the relative concentrations of individual lipid types. Understanding of the dependence of replication rates on physicochemical parameters opens a new direction in the study of prebiotic vesicles and lays the groundwork for future studies involving the competition between lipid vesicles for available amphiphilic monomers

  13. Self-control predicts exercise behavior by force of habit, a conceptual replication of Adriaanse et al.

    NARCIS (Netherlands)

    Gillebaart, Marleen; Adriaanse, Marieke A.

    2017-01-01

    A recent study suggests that habits play a mediating role in the association between trait self-control and eating behavior, supporting a notion of effortless processes in trait self-control (Adriaanse et al., 2014). We conceptually replicated this research in the area of exercise behavior,

  14. Cellular specificity of HIV-1 replication can be controlled by LTR sequences

    International Nuclear Information System (INIS)

    Reed-Inderbitzin, Edward; Maury, Wendy

    2003-01-01

    Two well-established determinants of retroviral tropism are envelope sequences that regulate entry and LTR sequences that can regulate viral expression in a cell-specific manner. Studies with human immunodeficiency virus-1 (HIV-1) have demonstrated that tropism of this virus maps primarily to variable envelope sequences. Studies have demonstrated that T cell and macrophage-specific transcription factor binding motifs exist in the upstream region of the LTR U3; however, the ability of the core enhancer/promoter proximal elements (two NF-κB and three Sp1 sites) to function well in macrophages and T cells have led many to conclude that HIV LTR sequences are not primary determinants of HIV tropism. To determine if cellular specificity could be imparted to HIV by the core enhancer elements, the enhancer/promoter proximal region of the HIV LTR was substituted with motifs that control gene expression in a myeloid-specific manner. The enhancer region from equine infectious anemia virus (EIAV) when substituted for the HIV enhancer/promoter proximal region was found to drive expression in a macrophage-specific manner and was responsive to HIV Tat. The addition of a 5' methylation-dependent binding site (MDBP) and a promoter proximal Sp1 motif increased expression without altering cellular specificity. Spacing between the promoter proximal region and the TATA box was also found to influence LTR activity. Infectivity studies using chimeric LTRs within the context of a dual-tropic infectious molecular clone established that these LTRs directed HIV replication and production of infectious virions in macrophages but not primary T cells or T cell lines. This investigation demonstrates that cellular specificity can be imparted onto HIV-1 replication at the level of viral transcription and not entry

  15. Automatic control systems engineering

    International Nuclear Information System (INIS)

    Shin, Yun Gi

    2004-01-01

    This book gives descriptions of automatic control for electrical electronics, which indicates history of automatic control, Laplace transform, block diagram and signal flow diagram, electrometer, linearization of system, space of situation, state space analysis of electric system, sensor, hydro controlling system, stability, time response of linear dynamic system, conception of root locus, procedure to draw root locus, frequency response, and design of control system.

  16. Phosphorylation of the Bacillus subtilis Replication Controller YabA Plays a Role in Regulation of Sporulation and Biofilm Formation.

    Science.gov (United States)

    García García, Tránsito; Ventroux, Magali; Derouiche, Abderahmane; Bidnenko, Vladimir; Correia Santos, Sara; Henry, Céline; Mijakovic, Ivan; Noirot-Gros, Marie-Françoise; Poncet, Sandrine

    2018-01-01

    Bacillus subtilis cells can adopt different life-styles in response to various environmental cues, including planktonic cells during vegetative growth, sessile cells during biofilm formation and sporulation. While switching life-styles, bacteria must coordinate the progression of their cell cycle with their physiological status. Our current understanding of the regulatory pathways controlling the decision-making processes and triggering developmental switches highlights a key role of protein phosphorylation. The regulatory mechanisms that integrate the bacterial chromosome replication status with sporulation involve checkpoint proteins that target the replication initiator DnaA or the kinase phosphorelay controlling the master regulator Spo0A. B. subtilis YabA is known to interact with DnaA to prevent over-initiation of replication during vegetative growth. Here, we report that YabA is phosphorylated by YabT, a Ser/Thr kinase expressed during sporulation and biofilm formation. The phosphorylation of YabA has no effect on replication initiation control but hyper-phosphorylation of YabA leads to an increase in sporulation efficiency and a strong inhibition of biofilm formation. We also provide evidence that YabA phosphorylation affects the level of Spo0A-P in cells. These results indicate that YabA is a multifunctional protein with a dual role in regulating replication initiation and life-style switching, thereby providing a potential mechanism for cross-talk and coordination of cellular processes during adaptation to environmental change.

  17. Phosphorylation of the Bacillus subtilis Replication Controller YabA Plays a Role in Regulation of Sporulation and Biofilm Formation

    Directory of Open Access Journals (Sweden)

    Tránsito García García

    2018-03-01

    Full Text Available Bacillus subtilis cells can adopt different life-styles in response to various environmental cues, including planktonic cells during vegetative growth, sessile cells during biofilm formation and sporulation. While switching life-styles, bacteria must coordinate the progression of their cell cycle with their physiological status. Our current understanding of the regulatory pathways controlling the decision-making processes and triggering developmental switches highlights a key role of protein phosphorylation. The regulatory mechanisms that integrate the bacterial chromosome replication status with sporulation involve checkpoint proteins that target the replication initiator DnaA or the kinase phosphorelay controlling the master regulator Spo0A. B. subtilis YabA is known to interact with DnaA to prevent over-initiation of replication during vegetative growth. Here, we report that YabA is phosphorylated by YabT, a Ser/Thr kinase expressed during sporulation and biofilm formation. The phosphorylation of YabA has no effect on replication initiation control but hyper-phosphorylation of YabA leads to an increase in sporulation efficiency and a strong inhibition of biofilm formation. We also provide evidence that YabA phosphorylation affects the level of Spo0A-P in cells. These results indicate that YabA is a multifunctional protein with a dual role in regulating replication initiation and life-style switching, thereby providing a potential mechanism for cross-talk and coordination of cellular processes during adaptation to environmental change.

  18. A checkpoint control orchestrates the replication of the two chromosomes of Vibrio cholerae

    DEFF Research Database (Denmark)

    Val, Marie-Eve; Marbouty, Martial; Martins, Francisco de Lemos

    2016-01-01

    Bacteria with multiple chromosomes represent up to 10% of all bacterial species. Unlike eukaryotes, these bacteria use chromosome-specific initiators for their replication. In all cases investigated, the machineries for secondary chromosome replication initiation are of plasmid origin. One of the...

  19. Effects of benzo[a]pyrene-DNA adducts on a reconstituted replication system

    International Nuclear Information System (INIS)

    Brown, W.C.; Romano, L.J.

    1991-01-01

    The authors have used a partially reconstituted replication system consisting of T7 DNA polymerase and T7 gene 4 protein to examine the effect of benzo[a]pyrene (B[a]P) adducts on DNA synthesis and gene 4 protein activities. The gene 4 protein is required for T7 DNA replication because of its ability to act as both a primase and helicase. They show here that total synthesis decreases as the level of adducts per molecule of DNA increases, suggesting that the B[a]P adducts are blocking an aspect of the replication process. By challenging synthesis on oligonucleotide-primed B[a]P-modified DNA with unmodified DNA, they present evidence that the T7 DNA polymerase freely dissociates after encountering an adduct. Prior studies have shown that the gene 4 protein alone does not dissociate from the template during translocation upon encountering an adduct. However, when gene 4 protein primed DNA synthesis is challenged, they observe an increase in synthesis but to a lesser extent than observed on oligonucleotide-primed synthesis. Finally, they have examined DNA synthesis on duplex templates and show the B[a]P adducts inhibit synthesis by the T7 DNA polymerase and gene 4 protein to the same extent regardless of whether the adducts are positioned in the leading or lagging strand, while synthesis by the polymerase alone is inhibited only when the adducts are in the template strand

  20. Precision digital control systems

    Science.gov (United States)

    Vyskub, V. G.; Rozov, B. S.; Savelev, V. I.

    This book is concerned with the characteristics of digital control systems of great accuracy. A classification of such systems is considered along with aspects of stabilization, programmable control applications, digital tracking systems and servomechanisms, and precision systems for the control of a scanning laser beam. Other topics explored are related to systems of proportional control, linear devices and methods for increasing precision, approaches for further decreasing the response time in the case of high-speed operation, possibilities for the implementation of a logical control law, and methods for the study of precision digital control systems. A description is presented of precision automatic control systems which make use of electronic computers, taking into account the existing possibilities for an employment of computers in automatic control systems, approaches and studies required for including a computer in such control systems, and an analysis of the structure of automatic control systems with computers. Attention is also given to functional blocks in the considered systems.

  1. Control of Genome Integrity by RFC Complexes; Conductors of PCNA Loading onto and Unloading from Chromatin during DNA Replication

    Directory of Open Access Journals (Sweden)

    Yasushi Shiomi

    2017-01-01

    Full Text Available During cell division, genome integrity is maintained by faithful DNA replication during S phase, followed by accurate segregation in mitosis. Many DNA metabolic events linked with DNA replication are also regulated throughout the cell cycle. In eukaryotes, the DNA sliding clamp, proliferating cell nuclear antigen (PCNA, acts on chromatin as a processivity factor for DNA polymerases. Since its discovery, many other PCNA binding partners have been identified that function during DNA replication, repair, recombination, chromatin remodeling, cohesion, and proteolysis in cell-cycle progression. PCNA not only recruits the proteins involved in such events, but it also actively controls their function as chromatin assembles. Therefore, control of PCNA-loading onto chromatin is fundamental for various replication-coupled reactions. PCNA is loaded onto chromatin by PCNA-loading replication factor C (RFC complexes. Both RFC1-RFC and Ctf18-RFC fundamentally function as PCNA loaders. On the other hand, after DNA synthesis, PCNA must be removed from chromatin by Elg1-RFC. Functional defects in RFC complexes lead to chromosomal abnormalities. In this review, we summarize the structural and functional relationships among RFC complexes, and describe how the regulation of PCNA loading/unloading by RFC complexes contributes to maintaining genome integrity.

  2. BSF control system

    International Nuclear Information System (INIS)

    Irie, Y.; Ishii, K.; Ninomiya, S.; Sasaki, H.; Sakai, I.

    1982-08-01

    The booster synchrotron utilization facility (BSF) is a facility which utilizes the four fifths of available beam pulses from the KEK booster synchrotron. The BSF control system includes the beam line control, interactions with the PS central control room and the experimental facilities, and the access control system. A brief description of the various components in the control system is given. (author)

  3. Mutant p53 perturbs DNA replication checkpoint control through TopBP1 and Treslin.

    Science.gov (United States)

    Liu, Kang; Lin, Fang-Tsyr; Graves, Joshua D; Lee, Yu-Ju; Lin, Weei-Chin

    2017-05-09

    Accumulating evidence supports the gain-of-function of mutant forms of p53 (mutp53s). However, whether mutp53 directly perturbs the DNA replication checkpoint remains unclear. Previously, we have demonstrated that TopBP1 forms a complex with mutp53s and mediates their gain-of-function through NF-Y and p63/p73. Akt phosphorylates TopBP1 and induces its oligomerization, which inhibits its ATR-activating function. Here we show that various contact and conformational mutp53s bypass Akt to induce TopBP1 oligomerization and attenuate ATR checkpoint response during replication stress. The effect on ATR response caused by mutp53 can be exploited in a synthetic lethality strategy, as depletion of another ATR activator, DNA2, in mutp53-R273H-expressing cancer cells renders cells hypersensitive to cisplatin. Expression of mutp53-R273H also makes cancer cells more sensitive to DNA2 depletion or DNA2 inhibitors. In addition to ATR-activating function during replication stress, TopBP1 interacts with Treslin in a Cdk-dependent manner to initiate DNA replication during normal growth. We find that mutp53 also interferes with TopBP1 replication function. Several contact, but not conformational, mutp53s enhance the interaction between TopBP1 and Treslin and promote DNA replication despite the presence of a Cdk2 inhibitor. Together, these data uncover two distinct mechanisms by which mutp53 enhances DNA replication: ( i ) Both contact and conformational mutp53s can bind TopBP1 and attenuate the checkpoint response to replication stress, and ( ii ) during normal growth, contact (but not conformational) mutp53s can override the Cdk2 requirement to promote replication by facilitating the TopBP1/Treslin interaction.

  4. Control system design method

    Science.gov (United States)

    Wilson, David G [Tijeras, NM; Robinett, III, Rush D.

    2012-02-21

    A control system design method and concomitant control system comprising representing a physical apparatus to be controlled as a Hamiltonian system, determining elements of the Hamiltonian system representation which are power generators, power dissipators, and power storage devices, analyzing stability and performance of the Hamiltonian system based on the results of the determining step and determining necessary and sufficient conditions for stability of the Hamiltonian system, creating a stable control system based on the results of the analyzing step, and employing the resulting control system to control the physical apparatus.

  5. A Novel System for Identification of Inhibitors of Rift Valley Fever Virus Replication

    OpenAIRE

    Piper, Mary E.; Gerrard, Sonja R.

    2010-01-01

    Rift Valley fever virus (RVFV) is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs) based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly ...

  6. Effects of feed loading on nitrogen balances and fish performance in replicated recirculating aquaculture systems

    DEFF Research Database (Denmark)

    Pedersen, Lars-Flemming; Suhr, Karin Isabel; Dalsgaard, Anne Johanne Tang

    2012-01-01

    This study investigated the effects of applying four fixed feed loadings to three replicated recirculating aquaculture systems (RAS) on water quality changes, nitrogenous balances and growth performance of rainbow trout (Oncorhynchus mykiss).Feed loadings ranged from 1.6 to 6.3kgfeed/m3 make-up...... water, with a constant make-up water renewal of 4.7% of total water volume per day in all twelve RAS. Fish densities ranged from 14 to 92kg/m3 during the prolonged trial of 10weeks. Selected water quality parameters were measured during two intensive sampling campaigns, evaluating biofilter...

  7. FFTF control system experience

    International Nuclear Information System (INIS)

    Warrick, R.P.

    1981-01-01

    The FFTF control systems provide control equipment for safe and efficient operation of the plant. For convenience, these systems will be divided into three parts for discussions: (1) Plant Protection System (PPS); (2) Plant Control System (PCS); and (3) General Observations. Performance of each of these systems is discussed

  8. A maize root tip system to study DNA replication programmes in somatic and endocycling nuclei during plant development.

    Science.gov (United States)

    Bass, Hank W; Wear, Emily E; Lee, Tae-Jin; Hoffman, Gregg G; Gumber, Hardeep K; Allen, George C; Thompson, William F; Hanley-Bowdoin, Linda

    2014-06-01

    The progress of nuclear DNA replication is complex in both time and space, and may reflect several levels of chromatin structure and 3-dimensional organization within the nucleus. To understand the relationship between DNA replication and developmental programmes, it is important to examine replication and nuclear substructure in different developmental contexts including natural cell-cycle progressions in situ. Plant meristems offer an ideal opportunity to analyse such processes in the context of normal growth of an organism. Our current understanding of large-scale chromosomal DNA replication has been limited by the lack of appropriate tools to visualize DNA replication with high resolution at defined points within S phase. In this perspective, we discuss a promising new system that can be used to visualize DNA replication in isolated maize (Zea mays L.) root tip nuclei after in planta pulse labelling with the thymidine analogue, 5-ethynyl-2'-deoxyuridine (EdU). Mixed populations of EdU-labelled nuclei are then separated by flow cytometry into sequential stages of S phase and examined directly using 3-dimensional deconvolution microscopy to characterize spatial patterns of plant DNA replication. Combining spatiotemporal analyses with studies of replication and epigenetic inheritance at the molecular level enables an integrated experimental approach to problems of mitotic inheritance and cellular differentiation. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  9. Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork

    OpenAIRE

    Rapp, Jordan B.; Ansbach, Alison B.; Noguchi, Chiaki; Noguchi, Eishi

    2009-01-01

    Replication of chromosomes involves a variety of replication proteins including DNA polymerases, DNA helicases, and other accessory factors. Many of these proteins are known to localize at replication forks and travel with them as components of the replisome complex. Other proteins do not move with replication forks but still play an essential role in DNA replication. Therefore, in order to understand the mechanisms of DNA replication and its controls, it is important to examine localization ...

  10. Wisdom Appliance Control System

    Science.gov (United States)

    Hendrick; Jheng, Jyun-Teng; Tsai, Chen-Chai; Liou, Jia-Wei; Wang, Zhi-Hao; Jong, Gwo-Jia

    2017-07-01

    Intelligent appliances wisdom involves security, home care, convenient and energy saving, but the home automation system is still one of the core unit, and also using micro-processing electronics technology to centralized and control the home electrical products and systems, such as: lighting, television, fan, air conditioning, stereo, it composed of front-controller systems and back-controller panels, user using front-controller to control command, and then through the back-controller to powered the device.

  11. Personal exposure control system

    International Nuclear Information System (INIS)

    Tanabe, Ken-ichi; Akashi, Michio

    1994-01-01

    Nuclear power stations are under strict radiation control. Exposure control for nuclear workers is the most important operation, and so carefully thought out measures are taken. This paper introduces Fuji Electric's personal exposure control system that meets strict exposure control and rationalizes control operations. The system has a merit that it can provide required information in an optimum form using the interconnection of a super minicomputer and exposure control facilities and realizes sophisticated exposure control operations. (author)

  12. Control of Initiation of DNA Replication in Bacillus subtilis and Escherichia coli

    Directory of Open Access Journals (Sweden)

    Katie H. Jameson

    2017-01-01

    Full Text Available Initiation of DNA Replication is tightly regulated in all cells since imbalances in chromosomal copy number are deleterious and often lethal. In bacteria such as Bacillus subtilis and Escherichia coli, at the point of cytokinesis, there must be two complete copies of the chromosome to partition into the daughter cells following division at mid-cell during vegetative growth. Under conditions of rapid growth, when the time taken to replicate the chromosome exceeds the doubling time of the cells, there will be multiple initiations per cell cycle and daughter cells will inherit chromosomes that are already undergoing replication. In contrast, cells entering the sporulation pathway in B. subtilis can do so only during a short interval in the cell cycle when there are two, and only two, chromosomes per cell, one destined for the spore and one for the mother cell. Here, we briefly describe the overall process of DNA replication in bacteria before reviewing initiation of DNA replication in detail. The review covers DnaA-directed assembly of the replisome at oriC and the multitude of mechanisms of regulation of initiation, with a focus on the similarities and differences between E. coli and B. subtilis.

  13. Control of Initiation of DNA Replication in Bacillus subtilis and Escherichia coli

    Science.gov (United States)

    Jameson, Katie H.; Wilkinson, Anthony J.

    2017-01-01

    Initiation of DNA Replication is tightly regulated in all cells since imbalances in chromosomal copy number are deleterious and often lethal. In bacteria such as Bacillus subtilis and Escherichia coli, at the point of cytokinesis, there must be two complete copies of the chromosome to partition into the daughter cells following division at mid-cell during vegetative growth. Under conditions of rapid growth, when the time taken to replicate the chromosome exceeds the doubling time of the cells, there will be multiple initiations per cell cycle and daughter cells will inherit chromosomes that are already undergoing replication. In contrast, cells entering the sporulation pathway in B. subtilis can do so only during a short interval in the cell cycle when there are two, and only two, chromosomes per cell, one destined for the spore and one for the mother cell. Here, we briefly describe the overall process of DNA replication in bacteria before reviewing initiation of DNA replication in detail. The review covers DnaA-directed assembly of the replisome at oriC and the multitude of mechanisms of regulation of initiation, with a focus on the similarities and differences between E. coli and B. subtilis. PMID:28075389

  14. The remote control system

    International Nuclear Information System (INIS)

    Jansweijer, P.P.M.

    1988-01-01

    The remote-control system is applied in order to control various signals in the car of the spectrometer at distance. The construction (hardware and software) as well as the operation of the system is described. (author). 20 figs

  15. The multi-replication protein A (RPA) system--a new perspective.

    Science.gov (United States)

    Sakaguchi, Kengo; Ishibashi, Toyotaka; Uchiyama, Yukinobu; Iwabata, Kazuki

    2009-02-01

    Replication protein A (RPA) complex has been shown, using both in vivo and in vitro approaches, to be required for most aspects of eukaryotic DNA metabolism: replication, repair, telomere maintenance and homologous recombination. Here, we review recent data concerning the function and biological importance of the multi-RPA complex. There are distinct complexes of RPA found in the biological kingdoms, although for a long time only one type of RPA complex was believed to be present in eukaryotes. Each complex probably serves a different role. In higher plants, three distinct large and medium subunits are present, but only one species of the smallest subunit. Each of these protein subunits forms stable complexes with their respective partners. They are paralogs as complex. Humans possess two paralogs and one analog of RPA. The multi-RPA system can be regarded as universal in eukaryotes. Among eukaryotic kingdoms, paralogs, orthologs, analogs and heterologs of many DNA synthesis-related factors, including RPA, are ubiquitous. Convergent evolution seems to be ubiquitous in these processes. Using recent findings, we review the composition and biological functions of RPA complexes.

  16. Control and automation systems

    International Nuclear Information System (INIS)

    Schmidt, R.; Zillich, H.

    1986-01-01

    A survey is given of the development of control and automation systems for energy uses. General remarks about control and automation schemes are followed by a description of modern process control systems along with process control processes as such. After discussing the particular process control requirements of nuclear power plants the paper deals with the reliability and availability of process control systems and refers to computerized simulation processes. The subsequent paragraphs are dedicated to descriptions of the operating floor, ergonomic conditions, existing systems, flue gas desulfurization systems, the electromagnetic influences on digital circuits as well as of light wave uses. (HAG) [de

  17. ISABELLE control system

    International Nuclear Information System (INIS)

    Humphrey, J.W.; Frankel, R.S.; Niederer, J.A.

    1980-01-01

    Design principles for the Brookhaven ISABELLE control intersecting storage ring accelerator are described. Principal features include a locally networked console and control computer complex, a system wide process data highway, and intelligent local device controllers. Progress to date is summarized

  18. Natural Killer p46 Controls Hepatitis B Virus Replication and Modulates Liver Inflammation.

    Directory of Open Access Journals (Sweden)

    Wanyu Li

    Full Text Available Natural killer (NK cells play an important role in hepatitis B virus (HBV infection control, and are regulated by a complex network of activating and inhibitory receptors. However, NK cell activity in HBV patients remains poorly understood. The objective of this study was to investigate the phenotypic and functional characteristics of circulating NK cells in patients during different chronic hepatitis B (CHB infection stages. We investigated NK cell phenotypes, receptor expression and function in 86 CHB patients and 20 healthy controls. NK cells were purified and NK cell subsets were characterized by flow cytometry. Cytotoxic activity (CD107a and interferon-gamma (IFN-γ secretion were examined, and Natural Killer p46 (NKP46 blockade and spontaneous NK cell cytolytic activity against K562, HepG2 and HepG2.215 cell lines was studied. Activating NKp46 receptor expression was higher in inactive HBsAg carriers when compared with other groups (p = 0.008. NKp46 expression negatively correlated with HBV DNA (R = -0.253, p = 0.049 and ALT (R = -0.256, p = 0.045 levels. CD107a was higher in immune-activated groups when compared with immune-tolerant groups (p = 0.039. CD107a expression was related to viral load (p = 0.02 and HBeAg status (p = 0.024. In vitro NKp46 blockade reduced NK cell cytolytic activity against HepG2 and HepG2.215 cell lines (p = 0.02; p = 0.039. Furthermore, NK cells from high viral load CHB patients displayed significantly lower specific cytolytic activity against anti-NKp46-loaded K562 targets (p = 0.0321. No significant differences were observed in IFN-γ secretion (p > 0.05. In conclusion, NKp46 expression regulates NK cell cytolytic function. NKp46 may moderate NK cell activity during HBV replication suppression and HBV-associated liver damage and may be critical for NK cell activity during CHB infection.

  19. Reactor control system. PWR

    International Nuclear Information System (INIS)

    2009-01-01

    At present, 23 units of PWR type reactors have been operated in Japan since the start of Mihama Unit 1 operation in 1970 and various improvements have been made to upgrade operability of power stations as well as reliability and safety of power plants. As the share of nuclear power increases, further improvements of operating performance such as load following capability will be requested for power stations with more reliable and safer operation. This article outlined the reactor control system of PWR type reactors and described the control performance of power plants realized with those systems. The PWR control system is characterized that the turbine power is automatic or manually controlled with request of the electric power system and then the nuclear power is followingly controlled with the change of core reactivity. The system mainly consists of reactor automatic control system (control rod control system), pressurizer pressure control system, pressurizer water level control system, steam generator water level control system and turbine bypass control system. (T. Tanaka)

  20. Multiple determinants controlling activation of yeast replication origins late in S phase.

    Science.gov (United States)

    Friedman, K L; Diller, J D; Ferguson, B M; Nyland, S V; Brewer, B J; Fangman, W L

    1996-07-01

    Analysis of a 131-kb segment of the left arm of yeast chromosome XIV beginning 157 kb from the telomere reveals four highly active origins of replication that initiate replication late in S phase. Previous work has shown that telomeres act as determinants for late origin activation. However, at least two of the chromosome XIV origins maintain their late activation time when located on large circular plasmids, indicating that late replication is independent of telomeres. Analysis of the replication time of plasmid derivatives containing varying amounts of chromosome XIV DNA show that a minimum of three chromosomal elements, distinct from each tested origin, contribute to late activation time. These late determinants are functionally equivalent, because duplication of one set of contributing sequences can compensate for the removal of another set. Furthermore, insertion of an origin that is normally early activated into this domain results in a shift to late activation, suggesting that the chromosome XIV origins are not unique in their ability to respond to the late determinants.

  1. Dynamics of DNA replication loops reveal temporal control of lagging-strand synthesis

    NARCIS (Netherlands)

    Hamdan, Samir M.; Loparo, Joseph J.; Takahashi, Masateru; Richardson, Charles C.; Oijen, Antoine M. van

    2009-01-01

    In all organisms, the protein machinery responsible for the replication of DNA, the replisome, is faced with a directionality problem. The antiparallel nature of duplex DNA permits the leading-strand polymerase to advance in a continuous fashion, but forces the lagging-strand polymerase to

  2. Prolonged control of replication-competent dual- tropic human immunodeficiency virus-1 following cessation of highly active antiretroviral therapy

    Directory of Open Access Journals (Sweden)

    Salgado Maria

    2011-12-01

    Full Text Available Abstract Background While initiation of highly active antiretroviral therapy (HAART during primary HIV-1 infection occasionally results in transient control of viral replication after treatment interruption, the vast majority of patients eventually experience a rebound in plasma viremia. Results Here we report a case of a patient who was started on HAART during symptomatic primary infection and who has subsequently maintained viral loads of + T cells. In addition, he does not have any known protective HLA alleles. Thus it is unlikely that he was destined to become a natural elite controller or suppressor. The mechanism of control of viral replication is unclear; he is infected with a CCR5/CXCR4 dual-tropic virus that is fully replication-competent in vitro. In addition, his spouse, who transmitted the virus to him, developed AIDS. The patient's CD4+ T cells are fully susceptible to HIV-1 infection, and he has low titers of neutralizing antibodies to heterologous and autologous HIV-1 isolates. Furthermore, his CD8+ T cells do not have potent HIV suppressive activity. Conclusion This report suggests that some patients may be capable of controlling pathogenic HIV-1 isolates for extended periods of time after the cessation of HAART through a mechanism that is distinct from the potent cytotoxic T lymphocyte (CTL mediated suppression that has been reported in many elite suppressors.

  3. Integrated control systems

    International Nuclear Information System (INIS)

    Smith, D.J.

    1991-01-01

    This paper reports that instrument manufacturers must develop standard network interfaces to pull together interrelated systems such as automatic start-up, optimization programs, and online diagnostic systems. In the past individual control system manufacturers have developed their own data highways with proprietary hardware and software designs. In the future, electric utilities will require that future systems, irrespective of manufacturer, should be able to communicate with each other. Until now the manufactures of control systems have not agreed on the standard high-speed data highway system. Currently, the Electric Power Research Institute (EPRI), in conjunction with several electric utilities and equipment manufactures, is working on developing a standard protocol for communicating between various manufacturers' control systems. According to N. Michael of Sargent and Lundy, future control room designs will require that more of the control and display functions be accessible from the control room through CRTs. There will be less emphasis on traditional hard-wired control panels

  4. A national control system

    International Nuclear Information System (INIS)

    Larsson, A.

    1975-01-01

    An effective control of nuclear fissionable material is dependent on three different kinds of control, the industry - laboratory management, a national control system and an international safeguards system. The national systems of control differ greatly between various industrialized countries. Two principal reasons for fact can be mentioned. The type and the amounts for nuclear material may be different depending upon the stage of development of the nuclear industry in the country in question. Another reason may be that the country may wish to establish a very elaborate national system of control in order to minimize the IAEA control as much as possible. The two safeguards agreements between the Agency and Sweden on one hand and the Agency and Japan on the other hand can serve as examples for the understanding of the latitude of the IAEA safeguards system under NPT due to the influence of the national control system. If it thus is apparent that the national control system is strongly interrelated to the international safeguards system it is equally influenced by the control and accountancy systems which exist at the nuclear plants and development laboratories. A detailed study of national control systems and their relations to plant management control would fall outside the scope of this article. Some important features will however be examined. (author)

  5. Intelligent control systems 1990

    International Nuclear Information System (INIS)

    Shoureshi, R.

    1991-01-01

    The field of artificial intelligence (Al) has generated many useful ideas and techniques that can be integrated into the design of control systems. It is believed and, for special cases, has been demonstrated, that integration of Al into control systems would provide the necessary tools for solving many of the complex problems that present control techniques and Al algorithms are unable to do, individually. However, this integration requires the development of basic understanding and new fundamentals to provide scientific bases for achievement of its potential. This book presents an overview of some of the latest research studies in the area of intelligent control systems. These papers present techniques for formulation of intelligent control, and development of the rule-based control systems. Papers present applications of control systems in nuclear power plants and HVAC systems

  6. Distributional Replication

    OpenAIRE

    Beare, Brendan K.

    2009-01-01

    Suppose that X and Y are random variables. We define a replicating function to be a function f such that f(X) and Y have the same distribution. In general, the set of replicating functions for a given pair of random variables may be infinite. Suppose we have some objective function, or cost function, defined over the set of replicating functions, and we seek to estimate the replicating function with the lowest cost. We develop an approach to estimating the cheapest replicating function that i...

  7. ALFA Detector Control System

    CERN Document Server

    Oleiro Seabra, Luis Filipe; The ATLAS collaboration

    2015-01-01

    ALFA (Absolute Luminosity For ATLAS) is one of the sub-detectors of ATLAS (A Toroidal LHC Apparatus). The ALFA system is composed by four stations installed in the LHC tunnel 240 m away from the ATLAS interaction point. Each station has a vacuum and ventilation system, movement control and all the required electronics for signal processing. The Detector Control System (DCS) provides control and monitoring of several components and ensures the safe operation of the detector contributing to good Data Quality. This paper describes the ALFA DCS system including a detector overview, operation aspects and hardware control through a SCADA system, WinCC OA.

  8. ALFA Detector Control System

    CERN Document Server

    Oleiro Seabra, Luis Filipe; The ATLAS collaboration

    2015-01-01

    ALFA (Absolute Luminosity For ATLAS) is one of the sub-detectors of ATLAS/LHC. The ALFA system is composed by two stations installed in the LHC tunnel 240 m away from each side of the ATLAS interaction point. Each station has a vacuum and ventilation system, movement control and all the required electronic for signal processing. The Detector Control System (DCS) provides control and monitoring of several components and ensures the safe operation of the detector contributing to good Data Quality. This paper describes the ALFA DCS system including a detector overview, operation aspects and hardware control through a SCADA system, WinCC OA.

  9. A modular control system

    International Nuclear Information System (INIS)

    Cruz, B.; Drexler, J.; Olcese, G.; Santome, D.

    1990-01-01

    The main objective of the modular control system is to provide the requirements to most of the processes supervision and control applications within the industrial automatization area. The design is based on distribution, modulation and expansion concepts. (Author) [es

  10. Applied Control Systems Design

    CERN Document Server

    Mahmoud, Magdi S

    2012-01-01

    Applied Control System Design examines several methods for building up systems models based on real experimental data from typical industrial processes and incorporating system identification techniques. The text takes a comparative approach to the models derived in this way judging their suitability for use in different systems and under different operational circumstances. A broad spectrum of control methods including various forms of filtering, feedback and feedforward control is applied to the models and the guidelines derived from the closed-loop responses are then composed into a concrete self-tested recipe to serve as a check-list for industrial engineers or control designers. System identification and control design are given equal weight in model derivation and testing to reflect their equality of importance in the proper design and optimization of high-performance control systems. Readers’ assimilation of the material discussed is assisted by the provision of problems and examples. Most of these e...

  11. The ILC control system

    International Nuclear Information System (INIS)

    Carwardine, J.; Saunders, C.; Arnold, N.; Lenkszus, F.; Rehlich, K.; Simrock, S.; Banerjee, b.; Chase, B.; Gottschalk, E.; Joireman, P.; Kasley, P.; Lackey, S.; McBride, P.; Pavlicek, V.; Patrick, J.; Votava, M.; Wolbers, S.; Furukawa, K.; Michizono, S.; Larson, R.S.; Downing, R.

    2007-01-01

    Since the last ICALEPCS, a small multi-region team has developed a reference design model for a control system for the International Linear Collider as part of the ILC Global Design Effort. The scale and performance parameters of the ILC accelerator require new thinking in regards to control system design. Technical challenges include the large number of accelerator systems to be controlled, the large scale of the accelerator facility, the high degree of automation needed during accelerator operations, and control system equipment requiring 'Five Nines' availability. The R and D path for high availability touches the control system hardware, software, and overall architecture, and extends beyond traditional interfaces into the technical systems. Software considerations for HA include fault detection through exhaustive out-of-band monitoring and automatic state migration to redundant systems, while the telecom industry's emerging ATCA standard - conceived, specified, and designed for High Availability - is being evaluated for suitability for ILC front-end electronics.

  12. Control systems engineering

    CERN Document Server

    Nise, Norman S

    1995-01-01

    This completely updated new edition shows how to use MATLAB to perform control-system calculations. Designed for the professional or engineering student who needs a quick and readable update on designing control systems, the text features a series of tightly focused examples that clearly illustrate each concept of designing control systems. Most chapters conclude with a detailed application from the two case studies that run throughout the book: an antenna asimuth control system and a submarine. The author also refers to many examples of design methods.

  13. Reverse Genetics System Demonstrates that Rotavirus Nonstructural Protein NSP6 Is Not Essential for Viral Replication in Cell Culture.

    Science.gov (United States)

    Komoto, Satoshi; Kanai, Yuta; Fukuda, Saori; Kugita, Masanori; Kawagishi, Takahiro; Ito, Naoto; Sugiyama, Makoto; Matsuura, Yoshiharu; Kobayashi, Takeshi; Taniguchi, Koki

    2017-11-01

    The use of overlapping open reading frames (ORFs) to synthesize more than one unique protein from a single mRNA has been described for several viruses. Segment 11 of the rotavirus genome encodes two nonstructural proteins, NSP5 and NSP6. The NSP6 ORF is present in the vast majority of rotavirus strains, and therefore the NSP6 protein would be expected to have a function in viral replication. However, there is no direct evidence of its function or requirement in the viral replication cycle yet. Here, taking advantage of a recently established plasmid-only-based reverse genetics system that allows rescue of recombinant rotaviruses entirely from cloned cDNAs, we generated NSP6-deficient viruses to directly address its significance in the viral replication cycle. Viable recombinant NSP6-deficient viruses could be engineered. Single-step growth curves and plaque formation of the NSP6-deficient viruses confirmed that NSP6 expression is of limited significance for RVA replication in cell culture, although the NSP6 protein seemed to promote efficient virus growth. IMPORTANCE Rotavirus is one of the most important pathogens of severe diarrhea in young children worldwide. The rotavirus genome, consisting of 11 segments of double-stranded RNA, encodes six structural proteins (VP1 to VP4, VP6, and VP7) and six nonstructural proteins (NSP1 to NSP6). Although specific functions have been ascribed to each of the 12 viral proteins, the role of NSP6 in the viral replication cycle remains unknown. In this study, we demonstrated that the NSP6 protein is not essential for viral replication in cell culture by using a recently developed plasmid-only-based reverse genetics system. This reverse genetics approach will be successfully applied to answer questions of great interest regarding the roles of rotaviral proteins in replication and pathogenicity, which can hardly be addressed by conventional approaches. Copyright © 2017 American Society for Microbiology.

  14. Discrete control systems

    CERN Document Server

    Okuyama, Yoshifumi

    2014-01-01

    Discrete Control Systems establishes a basis for the analysis and design of discretized/quantized control systemsfor continuous physical systems. Beginning with the necessary mathematical foundations and system-model descriptions, the text moves on to derive a robust stability condition. To keep a practical perspective on the uncertain physical systems considered, most of the methods treated are carried out in the frequency domain. As part of the design procedure, modified Nyquist–Hall and Nichols diagrams are presented and discretized proportional–integral–derivative control schemes are reconsidered. Schemes for model-reference feedback and discrete-type observers are proposed. Although single-loop feedback systems form the core of the text, some consideration is given to multiple loops and nonlinearities. The robust control performance and stability of interval systems (with multiple uncertainties) are outlined. Finally, the monograph describes the relationship between feedback-control and discrete ev...

  15. ‘The octet’: eight protein kinases that control mammalian DNA replication

    Directory of Open Access Journals (Sweden)

    Melvin L. Depamphilis

    2012-09-01

    Full Text Available Development of a fertilized human egg into an average sized adult requires about 29 trillion cell divisions, thereby producing enough DNA to stretch to the Sun and back 200 times (DePamphilis and Bell, 2011! Even more amazing is the fact that throughout these mitotic cell cycles, the human genome is duplicated once and only once each time a cell divides. If a cell accidentally begins to re-replicate its nuclear DNA prior to cell division, checkpoint pathways trigger apoptosis. And yet, some cells are developmentally programmed to respond to environmental cues by switching from mitotic cell cycles to endocycles, a process in which multiple S phases occur in the absence of either mitosis or cytokinesis. Endocycles allow production of viable, differentiated, polyploid cells that no longer proliferate. What is surprising is that among the 516 (Manning et al., 2002 to 557 (BioMart web site protein kinases encoded by the human genome, only eight regulate nuclear DNA replication directly. These are Cdk1, Cdk2, Cdk4, Cdk6, Cdk7, Cdc7, Chk1 and Chk2. Even more remarkable is the fact that only four of these enzymes (Cdk1, Cdk7, Cdc7 and Chk1 are essential for mammalian development. Here we describe how these protein kinases determine when DNA replication occurs during mitotic cell cycles, how mammalian cells switch from mitotic cell cycles to endocycles, and how cancer cells can be selectively targeted for destruction by inducing them to begin a second S phase before mitosis is complete.

  16. Perforin is detrimental to controlling [corrected] C. muridarum replication in vitro, but not in vivo.

    Directory of Open Access Journals (Sweden)

    Raymond M Johnson

    Full Text Available CD4 T cells are critical for clearing experimental Chlamydia muridarum genital tract infections. Two independent in vitro CD4 T cell mechanisms have been identified for terminating Chlamydia replication in epithelial cells. One mechanism, requiring IFN-γ and T cell-epithelial cell contact, terminates infection by triggering epithelial production of nitric oxide to chlamydiacidal levels; the second is dependent on T cell degranulation. We recently demonstrated that there are two independent in vivo clearance mechanisms singly sufficient for clearing genital tract infections within six weeks; one dependent on iNOS, the other on Plac8. Redundant genital tract clearance mechanisms bring into question negative results in single-gene knockout mice. Two groups have shown that perforin-knockout mice were not compromised in their ability to clear C. muridarum genital tract infections. Because cell lysis would be detrimental to epithelial nitric oxide production we hypothesized that perforin was not critical for iNOS-dependent clearance, but posited that perforin could play a role in Plac8-dependent clearance. We tested whether the Plac8-dependent clearance was perforin-dependent by pharmacologically inhibiting iNOS in perforin-knockout mice. In vitro we found that perforin was detrimental to iNOS-dependent CD4 T cell termination of Chlamydia replication in epithelial cells. In vivo, unexpectedly, clearance in perforin knockout mice was delayed to the end of week 7 regardless of iNOS status. The discordant in vitro/in vivo results suggest that the perforin's contribution to bacterial clearance in vivo is not though enhancing CD4 T cell termination of Chlamydia replication in epithelial cells, but likely via a mechanism independent of T cell-epithelial cell interactions.

  17. Biogas plant control system

    International Nuclear Information System (INIS)

    Balasevicius, L.; Dervinis, G.; Macerauskas, V.

    2002-01-01

    This paper presents intelligent control system for the pig farm biogas production process. The system uses a fuzzy logic models based on knowledge of experts and operators. Four fuzzy models are introduced. The adequacy of fuzzy models is verified using real data and MATLAB simulation. Proposed expert system is implemented into traditional SCADA system for biogas process prediction and failure analyzing. (authors)

  18. How a Small Family of Yeast IDPs Control Complicated Processes Related to DNA Replication

    DEFF Research Database (Denmark)

    Marabini, Riccardo

    Ribonucleotide reductase (RNR) and proliferating cell nuclear antigen (PCNA) are two essential proteins involved in DNA replication. RNR catalyzes the last and rate limiting step of the deoxyribonucleotide biosynthetic pathway. The dysregulation of RNR has been related to higher mutation rate...... characterized in budding and fission yeast. Within this protein family Dif1 (from S. cerevisiae) and Spd1 (from S. pombe) were analyzed in this study. These proteins were previously found to interact with and regulate the activity of RNR and Spd1 was also linked to PCNA dependent signaling for degradation...

  19. Replication Catastrophe

    DEFF Research Database (Denmark)

    Toledo, Luis; Neelsen, Kai John; Lukas, Jiri

    2017-01-01

    Proliferating cells rely on the so-called DNA replication checkpoint to ensure orderly completion of genome duplication, and its malfunction may lead to catastrophic genome disruption, including unscheduled firing of replication origins, stalling and collapse of replication forks, massive DNA...... breakage, and, ultimately, cell death. Despite many years of intensive research into the molecular underpinnings of the eukaryotic replication checkpoint, the mechanisms underlying the dismal consequences of its failure remain enigmatic. A recent development offers a unifying model in which the replication...... checkpoint guards against global exhaustion of rate-limiting replication regulators. Here we discuss how such a mechanism can prevent catastrophic genome disruption and suggest how to harness this knowledge to advance therapeutic strategies to eliminate cancer cells that inherently proliferate under...

  20. Control system design guide

    Energy Technology Data Exchange (ETDEWEB)

    Sellers, David; Friedman, Hannah; Haasl, Tudi; Bourassa, Norman; Piette, Mary Ann

    2003-05-01

    The ''Control System Design Guide'' (Design Guide) provides methods and recommendations for the control system design process and control point selection and installation. Control systems are often the most problematic system in a building. A good design process that takes into account maintenance, operation, and commissioning can lead to a smoothly operating and efficient building. To this end, the Design Guide provides a toolbox of templates for improving control system design and specification. HVAC designers are the primary audience for the Design Guide. The control design process it presents will help produce well-designed control systems that achieve efficient and robust operation. The spreadsheet examples for control valve schedules, damper schedules, and points lists can streamline the use of the control system design concepts set forth in the Design Guide by providing convenient starting points from which designers can build. Although each reader brings their own unique questions to the text, the Design Guide contains information that designers, commissioning providers, operators, and owners will find useful.

  1. Control rod shutdown system

    International Nuclear Information System (INIS)

    Miyamoto, Yoshiyuki; Higashigawa, Yuichi.

    1996-01-01

    The present invention provides a control rod terminating system in a BWR type nuclear power plant, which stops an induction electric motor as rapidly as possible to terminate the control rods. Namely, the control rod stopping system controls reactor power by inserting/withdrawing control rods into a reactor by driving them by the induction electric motor. The system is provided with a control device for controlling the control rods and a control device for controlling the braking device. The control device outputs a braking operation signal for actuating the braking device during operation of the control rods to stop the operation of the control rods. Further, the braking device has at least two kinds of breaks, namely, a first and a second brakes. The two kinds of brakes are actuated by receiving the brake operation signals at different timings. The brake device is used also for keeping the control rods after the stopping. Even if a stopping torque of each of the breaks is small, different two kinds of brakes are operated at different timings thereby capable of obtaining a large stopping torque as a total. (I.S.)

  2. Systems and Control Engineering

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 4; Issue 5. Systems and Control Engineering - Control Systems-Analysis and Design. A Rama Kalyan J R Vengateswaran. General Article Volume 4 Issue 5 May 1999 pp 88-94 ...

  3. Spacecraft momentum control systems

    CERN Document Server

    Leve, Frederick A; Peck, Mason A

    2015-01-01

    The goal of this book is to serve both as a practical technical reference and a resource for gaining a fuller understanding of the state of the art of spacecraft momentum control systems, specifically looking at control moment gyroscopes (CMGs). As a result, the subject matter includes theory, technology, and systems engineering. The authors combine material on system-level architecture of spacecraft that feature momentum-control systems with material about the momentum-control hardware and software. This also encompasses material on the theoretical and algorithmic approaches to the control of space vehicles with CMGs. In essence, CMGs are the attitude-control actuators that make contemporary highly agile spacecraft possible. The rise of commercial Earth imaging, the advances in privately built spacecraft (including small satellites), and the growing popularity of the subject matter in academic circles over the past decade argues that now is the time for an in-depth treatment of the topic. CMGs are augmented ...

  4. Neutron generator control system

    International Nuclear Information System (INIS)

    Peelman, H.E.; Bridges, J.R.

    1981-01-01

    A method is described of controlling the neutron output of a neutron generator tube used in neutron well logging. The system operates by monitoring the target beam current and comparing a function of this current with a reference voltage level to develop a control signal used in a series regulator to control the replenisher current of the neutron generator tube. (U.K.)

  5. CD11c controls herpes simplex virus 1 responses to limit virus replication during primary infection.

    Science.gov (United States)

    Allen, Sariah J; Mott, Kevin R; Chentoufi, Aziz A; BenMohamed, Lbachir; Wechsler, Steven L; Ballantyne, Christie M; Ghiasi, Homayon

    2011-10-01

    CD11c is expressed on the surface of dendritic cells (DCs) and is one of the main markers for identification of DCs. DCs are the effectors of central innate immune responses, but they also affect acquired immune responses to infection. However, how DCs influence the efficacy of adaptive immunity is poorly understood. Here, we show that CD11c(+) DCs negatively orchestrate both adaptive and innate immunity against herpes simplex virus type 1 (HSV-1) ocular infection. The effectiveness and quantity of virus-specific CD8(+) T cell responses are increased in CD11c-deficient animals. In addition, the levels of CD83, CD11b, alpha interferon (IFN-α), and IFN-β, but not IFN-γ, were significantly increased in CD11c-deficient animals. Higher levels of IFN-α, IFN-β, and CD8(+) T cells in the CD11c-deficient mice may have contributed to lower virus replication in the eye and trigeminal ganglia (TG) during the early period of infection than in wild-type mice. However, the absence of CD11c did not influence survival, severity of eye disease, or latency. Our studies provide for the first time evidence that CD11c expression may abrogate the ability to reduce primary virus replication in the eye and TG via higher activities of type 1 interferon and CD8(+) T cell responses.

  6. Dynamic Systems and Control Engineering

    International Nuclear Information System (INIS)

    Kim, Jong Seok

    1994-02-01

    This book deals with introduction of dynamic system and control engineering, frequency domain modeling of dynamic system, temporal modeling of dynamic system, typical dynamic system and automatic control device, performance and stability of control system, root locus analysis, analysis of frequency domain dynamic system, design of frequency domain dynamic system, design and analysis of space, space of control system and digital control system such as control system design of direct digital and digitalization of consecutive control system.

  7. Dynamic Systems and Control Engineering

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jong Seok

    1994-02-15

    This book deals with introduction of dynamic system and control engineering, frequency domain modeling of dynamic system, temporal modeling of dynamic system, typical dynamic system and automatic control device, performance and stability of control system, root locus analysis, analysis of frequency domain dynamic system, design of frequency domain dynamic system, design and analysis of space, space of control system and digital control system such as control system design of direct digital and digitalization of consecutive control system.

  8. Drone Control System

    Science.gov (United States)

    1983-01-01

    Drones, subscale vehicles like the Firebees, and full scale retired military aircraft are used to test air defense missile systems. The DFCS (Drone Formation Control System) computer, developed by IBM (International Business Machines) Federal Systems Division, can track ten drones at once. A program called ORACLS is used to generate software to track and control Drones. It was originally developed by Langley and supplied by COSMIC (Computer Software Management and Information Center). The program saved the company both time and money.

  9. Magnetic spectrometer control system

    International Nuclear Information System (INIS)

    Lecca, L.A.; Di Paolo, Hugo; Fernandez Niello, Jorge O.; Marti, Guillermo V; Pacheco, Alberto J.; Ramirez, Marcelo

    2003-01-01

    The design and implementation of a new computerized control system for the several devices of the magnetic spectrometer at TANDAR Laboratory is described. This system, as a main difference from the preexisting one, is compatible with almost any operating systems of wide spread use available in PC. This allows on-line measurement and control of all signals from any terminal of a computer network. (author)

  10. HYBRID VEHICLE CONTROL SYSTEM

    Directory of Open Access Journals (Sweden)

    V. Dvadnenko

    2016-06-01

    Full Text Available The hybrid vehicle control system includes a start–stop system for an internal combustion engine. The system works in a hybrid mode and normal vehicle operation. To simplify the start–stop system, there were user new possibilities of a hybrid car, which appeared after the conversion. Results of the circuit design of the proposed system of basic blocks are analyzed.

  11. The control system

    International Nuclear Information System (INIS)

    1988-01-01

    The present control system has matured both in terms of age and capacity. Thus a new system based on a local area network (LAN) is being developed. A pilot project has been started but, owing to difficulties encountered with the present operating system used with the microprocessors, it has become necessary to reconsider the choice of operating system. A recently-released multi-tasking operating system that runs on the existing hardware has been chosen. 1 fig

  12. Drug repurposing to target Ebola virus replication and virulence using structural systems pharmacology.

    Science.gov (United States)

    Zhao, Zheng; Martin, Che; Fan, Raymond; Bourne, Philip E; Xie, Lei

    2016-02-18

    The recent outbreak of Ebola has been cited as the largest in history. Despite this global health crisis, few drugs are available to efficiently treat Ebola infections. Drug repurposing provides a potentially efficient solution to accelerating the development of therapeutic approaches in response to Ebola outbreak. To identify such candidates, we use an integrated structural systems pharmacology pipeline which combines proteome-scale ligand binding site comparison, protein-ligand docking, and Molecular Dynamics (MD) simulation. One thousand seven hundred and sixty-six FDA-approved drugs and 259 experimental drugs were screened to identify those with the potential to inhibit the replication and virulence of Ebola, and to determine the binding modes with their respective targets. Initial screening has identified a number of promising hits. Notably, Indinavir; an HIV protease inhibitor, may be effective in reducing the virulence of Ebola. Additionally, an antifungal (Sinefungin) and several anti-viral drugs (e.g. Maraviroc, Abacavir, Telbivudine, and Cidofovir) may inhibit Ebola RNA-directed RNA polymerase through targeting the MTase domain. Identification of safe drug candidates is a crucial first step toward the determination of timely and effective therapeutic approaches to address and mitigate the impact of the Ebola global crisis and future outbreaks of pathogenic diseases. Further in vitro and in vivo testing to evaluate the anti-Ebola activity of these drugs is warranted.

  13. Design Optimization of Time- and Cost-Constrained Fault-Tolerant Embedded Systems with Checkpointing and Replication

    DEFF Research Database (Denmark)

    Pop, Paul; Izosimov, Viacheslav; Eles, Petru

    2009-01-01

    We present an approach to the synthesis of fault-tolerant hard real-time systems for safety-critical applications. We use checkpointing with rollback recovery and active replication for tolerating transient faults. Processes and communications are statically scheduled. Our synthesis approach deci...

  14. System control and communication

    International Nuclear Information System (INIS)

    Bindner, H.; Oestergaard, J.

    2005-01-01

    Rapid and ongoing development in the energy sector has consequences for system control at all levels. In relation to system control and communication the control system is challenged in five important ways: 1) Expectations for security of supply, robustness and vulnerability are becoming more stringent, and the control system plays a big part in meeting these expectations. 2) Services are becoming increasingly based on markets that involve the transmission system operators (TSOs), generators and distribution companies. Timely, accurate and secure communication is essential to the smooth running of the markets. 3) Adding large amounts of renewable energy (RE) to the mix is a challenge for control systems because of the intermittent availability of many RE sources. 4) Increasing the number of active components in the system, such as small CHP plants, micro-CHP and intelligent loads, means that the system control will be much more complex. 5) In the future it is likely that power, heat, gas, transport and communication systems will be tighter coupled and interact much more. (au)

  15. The CEBAF control system

    International Nuclear Information System (INIS)

    Watson, W.A. III.

    1995-01-01

    CEBAF has recently upgraded its accelerator control system to use EPICS, a control system toolkit being developed by a collaboration among laboratories in the US and Europe. The migration to EPICS has taken place during a year of intense commissioning activity, with new and old control systems operating concurrently. Existing CAMAC hardware was preserved by adding a CAMAC serial highway link to VME; newer hardware developments are now primarily in VME. Software is distributed among three tiers of computers: first, workstations and X terminals for operator interfaces and high level applications; second, VME single board computers for distributed access to hardware and for local control processing; third, embedded processors where needed for faster closed loop operation. This system has demonstrated the ability to scale EPICS to controlling thousands of devices, including hundreds of embedded processors, with control distributed among dozens of VME processors executing more than 125,000 EPICS database records. To deal with the large size of the control system, CEBAF has integrated an object oriented database, providing data management capabilities for both low level I/O and high level machine modeling. A new callable interface which is control system independent permits access to live EPICS data, data in other Unix processes, and data contained in the object oriented database

  16. DNA replication and cancer

    DEFF Research Database (Denmark)

    Boyer, Anne-Sophie; Walter, David; Sørensen, Claus Storgaard

    2016-01-01

    A dividing cell has to duplicate its DNA precisely once during the cell cycle to preserve genome integrity avoiding the accumulation of genetic aberrations that promote diseases such as cancer. A large number of endogenous impacts can challenge DNA replication and cells harbor a battery of pathways...... causing DNA replication stress and genome instability. Further, we describe cellular and systemic responses to these insults with a focus on DNA replication restart pathways. Finally, we discuss the therapeutic potential of exploiting intrinsic replicative stress in cancer cells for targeted therapy....

  17. Load Control System Reliability

    Energy Technology Data Exchange (ETDEWEB)

    Trudnowski, Daniel [Montana Tech of the Univ. of Montana, Butte, MT (United States)

    2015-04-03

    This report summarizes the results of the Load Control System Reliability project (DOE Award DE-FC26-06NT42750). The original grant was awarded to Montana Tech April 2006. Follow-on DOE awards and expansions to the project scope occurred August 2007, January 2009, April 2011, and April 2013. In addition to the DOE monies, the project also consisted of matching funds from the states of Montana and Wyoming. Project participants included Montana Tech; the University of Wyoming; Montana State University; NorthWestern Energy, Inc., and MSE. Research focused on two areas: real-time power-system load control methodologies; and, power-system measurement-based stability-assessment operation and control tools. The majority of effort was focused on area 2. Results from the research includes: development of fundamental power-system dynamic concepts, control schemes, and signal-processing algorithms; many papers (including two prize papers) in leading journals and conferences and leadership of IEEE activities; one patent; participation in major actual-system testing in the western North American power system; prototype power-system operation and control software installed and tested at three major North American control centers; and, the incubation of a new commercial-grade operation and control software tool. Work under this grant certainly supported the DOE-OE goals in the area of “Real Time Grid Reliability Management.”

  18. ISTTOK control system upgrade

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Ivo S., E-mail: ivoc@ipfn.ist.utl.pt; Duarte, Paulo; Fernandes, Horácio; Valcárcel, Daniel F.; Carvalho, Pedro J.; Silva, Carlos; Duarte, André S.; Neto, André; Sousa, Jorge; Batista, António J.N.; Carvalho, Bernardo B.

    2013-10-15

    Highlights: •ISTTOK fast controller. •All real-time diagnostic and actuators were integrated in the control platform. •100 μs control cycle under the MARTe framework. •The ISTTOK control system upgrade provides reliable operation with an improved operational space. -- Abstract: The ISTTOK tokamak (Ip = 4 kA, BT = 0.5 T, R = 0.46 m, a = 0.085 m) is one of the few tokamaks with regular alternate plasma current (AC) discharges scientific programme. In order to improve the discharge stability and to increase the number of AC discharge cycles a novel control system was developed. The controller acquires data from 50 analog-to-digital converter (ADC) channels of real-time diagnostics and measurements: tomography, Mirnov coils, interferometer, electric probes, sine and cosine probes, bolometer, current delivered by the power supplies, loop voltage and plasma current. The system has a control cycle of 100 μs during which it reads all the diagnostics connected to the advanced telecommunications computing architecture (ATCA) digitizers and sends the control reference to ISTTOK actuators. The controller algorithms are executed on an Intel{sup ®} Q8200 chip with 4 cores running at 2.33 GHz and connected to the I/O interfaces through an ATCA based environment. The real-time control system was programmed in C++ on top of the Multi-threaded Application Real-Time executor (MARTe). To extend the duration of the AC discharges and the plasma stability a new magnetising field power supply was commissioned and the horizontal and vertical field power supplies were also upgraded. The new system also features a user-friendly interface based on HyperText Markup Language (HTML) and Javascript to configure the controller parameters. This paper presents the ISTTOK control system and the consequent update of real-time diagnostics and actuators.

  19. ISTTOK control system upgrade

    International Nuclear Information System (INIS)

    Carvalho, Ivo S.; Duarte, Paulo; Fernandes, Horácio; Valcárcel, Daniel F.; Carvalho, Pedro J.; Silva, Carlos; Duarte, André S.; Neto, André; Sousa, Jorge; Batista, António J.N.; Carvalho, Bernardo B.

    2013-01-01

    Highlights: •ISTTOK fast controller. •All real-time diagnostic and actuators were integrated in the control platform. •100 μs control cycle under the MARTe framework. •The ISTTOK control system upgrade provides reliable operation with an improved operational space. -- Abstract: The ISTTOK tokamak (Ip = 4 kA, BT = 0.5 T, R = 0.46 m, a = 0.085 m) is one of the few tokamaks with regular alternate plasma current (AC) discharges scientific programme. In order to improve the discharge stability and to increase the number of AC discharge cycles a novel control system was developed. The controller acquires data from 50 analog-to-digital converter (ADC) channels of real-time diagnostics and measurements: tomography, Mirnov coils, interferometer, electric probes, sine and cosine probes, bolometer, current delivered by the power supplies, loop voltage and plasma current. The system has a control cycle of 100 μs during which it reads all the diagnostics connected to the advanced telecommunications computing architecture (ATCA) digitizers and sends the control reference to ISTTOK actuators. The controller algorithms are executed on an Intel ® Q8200 chip with 4 cores running at 2.33 GHz and connected to the I/O interfaces through an ATCA based environment. The real-time control system was programmed in C++ on top of the Multi-threaded Application Real-Time executor (MARTe). To extend the duration of the AC discharges and the plasma stability a new magnetising field power supply was commissioned and the horizontal and vertical field power supplies were also upgraded. The new system also features a user-friendly interface based on HyperText Markup Language (HTML) and Javascript to configure the controller parameters. This paper presents the ISTTOK control system and the consequent update of real-time diagnostics and actuators

  20. The role of genes controlling the replication and cell division in the repair of radiation damage in Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Zhestyanikov, V D; Svetlova, M P; Tomilin, N V; Savel' eva, G E [AN SSSR, Leningrad. Inst. Tsitologii

    1975-01-01

    Mutations in genes controlling the replication (dnaEsup(ts), dnaBsup(ts), dnaGsup(ts) and cell division (lon) in Escherichia coli prevent the rejoining of the gamma radiation-induced single-strand breaks (dnaE in combination with polA1 mutation and dnaG at the restrictive temperature) and effective postreplication DNA repair in UV-irradiated cells (dnaG at the non-permissive temperature and lon mutation) and decrease the survival of UV- and gamma-irradiated bacteria.

  1. Control system integration

    CERN Document Server

    Shea, T J

    2008-01-01

    This lecture begins with a definition of an accelerator control system, and then reviews the control system architectures that have been deployed at the larger accelerator facilities. This discussion naturally leads to identification of the major subsystems and their interfaces. We shall explore general strategies for integrating intelligent devices and signal processing subsystems based on gate arrays and programmable DSPs. The following topics will also be covered: physical packaging; timing and synchronization; local and global communication technologies; interfacing to machine protection systems; remote debugging; configuration management and source code control; and integration of commercial software tools. Several practical realizations will be presented.

  2. The Epicure Control System

    International Nuclear Information System (INIS)

    Dambik, E.; Kline, D.; West, R.

    1993-09-01

    The Epicure Control System supports the Fermilab fixed target physics program. The system is distributed across a network of many different types of components. The use of multiple layers on interfaces for communication between logical tasks fits the client-server model. Physical devices are read and controlled using symbolic references entered into a database with an editor utility. The database system consists of a central portion containing all device information and optimized portions distributed among many nodes. Updates to the database are available throughout the system within minutes after being requested

  3. Control systems under attack?

    CERN Document Server

    Lüders, Stefan

    2005-01-01

    The enormous growth of the Internet during the last decade offers new means to share and distribute both information and data. In Industry, this results in a rapprochement of the production facilities, i.e. their Process Control and Automation Systems, and the data warehouses. At CERN, the Internet opens the possibility to monitor and even control (parts of) the LHC and its four experiments remotely from anywhere in the world. However, the adoption of standard IT technologies to Distributed Process Control and Automation Systems exposes inherent vulnerabilities to the world. The Teststand On Control System Security at CERN (TOCSSiC) is dedicated to explore the vulnerabilities of arbitrary Commercial-Of-The-Shelf hardware devices connected to standard Ethernet. As such, TOCSSiC should discover their vulnerabilities, point out areas of lack of security, and address areas of improvement which can then be confidentially communicated to manufacturers. This paper points out risks of accessing the Control and Automa...

  4. Tautological control systems

    CERN Document Server

    Lewis, Andrew D

    2014-01-01

    This brief presents a description of a new modelling framework for nonlinear/geometric control theory. The framework is intended to be—and shown to be—feedback-invariant. As such, Tautological Control Systems provides a platform for understanding fundamental structural problems in geometric control theory. Part of the novelty of the text stems from the variety of regularity classes, e.g., Lipschitz, finitely differentiable, smooth, real analytic, with which it deals in a comprehensive and unified manner. The treatment of the important real analytic class especially reflects recent work on real analytic topologies by the author. Applied mathematicians interested in nonlinear and geometric control theory will find this brief of interest as a starting point for work in which feedback invariance is important. Graduate students working in control theory may also find Tautological Control Systems to be a stimulating starting point for their research.

  5. Reset Control Systems

    CERN Document Server

    Baños, Alfonso

    2012-01-01

    Reset Control Systems addresses the analysis for reset control treating both its basic form which requires only that the state of the controller be reinitialized to zero (the reset action) each time the tracking error crosses zero (the reset condition), and some useful variations of the reset action (partial reset with fixed or variable reset percentage) and of the reset condition (fixed or variable reset band and anticipative reset). The issues regarding reset control – concepts and motivation; analysis tools; and the application of design methodologies to real-world examples – are given comprehensive coverage. The text opens with an historical perspective which moves from the seminal work of the Clegg integrator and Horowitz FORE to more recent approaches based on impulsive/hybrid control systems and explains the motivation for reset compensation. Preliminary material dealing with notation, basic definitions and results, and with the definition of the control problem under study is also included. The fo...

  6. Flight control actuation system

    Science.gov (United States)

    Wingett, Paul T. (Inventor); Gaines, Louie T. (Inventor); Evans, Paul S. (Inventor); Kern, James I. (Inventor)

    2006-01-01

    A flight control actuation system comprises a controller, electromechanical actuator and a pneumatic actuator. During normal operation, only the electromechanical actuator is needed to operate a flight control surface. When the electromechanical actuator load level exceeds 40 amps positive, the controller activates the pneumatic actuator to offset electromechanical actuator loads to assist the manipulation of flight control surfaces. The assistance from the pneumatic load assist actuator enables the use of an electromechanical actuator that is smaller in size and mass, requires less power, needs less cooling processes, achieves high output forces and adapts to electrical current variations. The flight control actuation system is adapted for aircraft, spacecraft, missiles, and other flight vehicles, especially flight vehicles that are large in size and travel at high velocities.

  7. Reactor limit control system

    International Nuclear Information System (INIS)

    Rubbel, F.E.

    1982-01-01

    The very extensive use of limitations in the operational field between protection system and closed-loop controls is an important feature of German understanding of operational safety. The design of limitations is based on very large activities in the computational field but mostly on the high level of the plant-wide own commissioning experience of a turnkey contractor. Limitations combine intelligence features of closed-loop controls with the high availability of protection systems. (orig.)

  8. Fluid flow control system

    International Nuclear Information System (INIS)

    Rion, Jacky.

    1982-01-01

    Fluid flow control system featuring a series of grids placed perpendicular to the fluid flow direction, characterized by the fact that it is formed of a stack of identical and continuous grids, each of which consists of identical meshes forming a flat lattice. The said meshes are offset from one grid to the next. This system applies in particular to flow control of the coolant flowing at the foot of an assembly of a liquid metal cooled nuclear reactor [fr

  9. Internal control system

    OpenAIRE

    Pavésková, Ivana

    2014-01-01

    Dissertation focuse on the internal control system in the enterprises, aims to map the control system by focusing on the purchasing department. I focused on the purchasing process, because with an increasing trends of outsourcing services and the increasing interconnectedness of enterprises increases the risk of fraud currently in the purchasing process. To the research was selected the sample of companies from the banking and non-banking environment, to which were sent a questionnaire focusi...

  10. Systems and Control Engineering

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 4; Issue 1. Systems and Control Engineering - Notions of Control. A Rama Kalyan J R Vengateswaran. General Article Volume 4 Issue 1 January 1999 pp 45-52. Fulltext. Click here to view fulltext PDF. Permanent link:

  11. Controllability of nilpotent systems

    International Nuclear Information System (INIS)

    Bravo, V.A.; Martin, L.S.

    1993-02-01

    The purpose of this paper is to investigate algebraic conditions which give information about the controllability of invariant control systems on nilpotent Lie groups. With the same purpose, the authors use the co-adjoint representation and define the concept of symplectic vectors. We study the existence of these objects to analyze the controllability. In particular, we obtain a characterization when G is simply connected. (author). 9 refs

  12. A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay

    Science.gov (United States)

    Aloia, Amanda L.; Duffy, Lisa; Pak, Vladimir; Lee, KyeongEun; Sanchez-Martinez, Silvia; Derse, David; Heidecker, Gisela; Cornetta, Kenneth; Rein, Alan

    2012-01-01

    While novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+L− or marker rescue assay, while standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+L− assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing. PMID:22402321

  13. Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System

    Directory of Open Access Journals (Sweden)

    Shuxuan Li

    2018-01-01

    Full Text Available Zika virus (ZIKV has been defined as a teratogenic pathogen behind the increased number of cases of microcephaly in French Polynesia, Brazil, Puerto Rico, and other South American countries. Experimental studies using animal models have achieved tremendous insight into understanding the viral pathogenesis, transmission, teratogenic mechanisms, and virus–host interactions. However, the animals used in published investigations are mostly interferon (IFN-compromised, either genetically or via antibody treatment. Herein, we studied ZIKV infection in IFN-competent mice using African (MR766 and Asian strains (PRVABC59 and SZ-WIV01. After testing four different species of mice, we found that BALB/c neonatal mice were resistant to ZIKV infection, that Kunming, ICR and C57BL/6 neonatal mice were fatally susceptible to ZIKV infection, and that the fatality of C57BL/6 neonates from 1 to 3 days old were in a viral dose-dependent manner. The size and weight of the brain were significantly reduced, and the ZIKV-infected mice showed neuronal symptoms such as hind-limb paralysis, tremor, and poor balance during walking. Pathologic and immunofluorescent experiments revealed that ZIKV infected different areas of the central nervous system (CNS including gray matter, hippocampus, cerebral cortex, and spinal cord, but not olfactory bulb. Interestingly, ZIKV replicated in multiple organs and resulted in pathogenesis in liver and testis, implying that ZIKV infection may engender a high health risk in neonates by postnatal infection. In summary, we investigated ZIKV pathogenesis using an animal model that is not IFN-compromised.

  14. Controlling cell adhesion via replication of laser micro/nano-textured surfaces on polymers

    Energy Technology Data Exchange (ETDEWEB)

    Koufaki, Niki; Ranella, Anthi; Barberoglou, Marios; Psycharakis, Stylianos; Fotakis, Costas; Stratakis, Emmanuel [Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology-Hellas (FORTH), 711 10, Heraklion, Crete (Greece); Aifantis, Katerina E, E-mail: stratak@iesl.forth.gr [Lab of Mechanics and Materials, Aristotle University of Thessaloniki, Thessaloniki (Greece)

    2011-12-15

    The aim of this study is to investigate cell adhesion and viability on highly rough polymeric surfaces with gradient roughness ratios and wettabilities prepared by microreplication of laser micro/nano-textured Si surfaces. Negative replicas on polydimethylsiloxane as well as positive ones on a photocurable (organically modified ceramic) and a biodegradable (poly(lactide-co-glycolide)) polymer have been successfully reproduced. The final culture substrates comprised from forests of micron-sized conical spikes exhibiting a range of roughness ratios and wettabilities, was achieved by changing the laser fluence used to fabricate the original template surfaces. Cell culture experiments were performed with the fibroblast NIH/3T3 and PC12 neuronal cell lines in order to investigate how these surfaces are capable of modulating different types of cellular responses including, viability, adhesion and morphology. The results showed a preferential adhesion of both cell types on the microstructured surfaces compared to the unstructured ones. In particular, the fibroblast NIH/3T3 cells show optimal adhesion for small roughness ratios, independent of the surface wettability and polymer type, indicating a non-monotonic dependence of cell adhesion on surface energy. In contrast, the PC12 cells were observed to adhere well to the patterned surfaces independent of the roughness ratio and wettability. These experimental findings are correlated with micromechanical measurements performed on the unstructured and replicated surfaces and discussed on the basis of previous observations describing the relation of cell response to surface energy and rigidity.

  15. Controlling cell adhesion via replication of laser micro/nano-textured surfaces on polymers

    International Nuclear Information System (INIS)

    Koufaki, Niki; Ranella, Anthi; Barberoglou, Marios; Psycharakis, Stylianos; Fotakis, Costas; Stratakis, Emmanuel; Aifantis, Katerina E

    2011-01-01

    The aim of this study is to investigate cell adhesion and viability on highly rough polymeric surfaces with gradient roughness ratios and wettabilities prepared by microreplication of laser micro/nano-textured Si surfaces. Negative replicas on polydimethylsiloxane as well as positive ones on a photocurable (organically modified ceramic) and a biodegradable (poly(lactide-co-glycolide)) polymer have been successfully reproduced. The final culture substrates comprised from forests of micron-sized conical spikes exhibiting a range of roughness ratios and wettabilities, was achieved by changing the laser fluence used to fabricate the original template surfaces. Cell culture experiments were performed with the fibroblast NIH/3T3 and PC12 neuronal cell lines in order to investigate how these surfaces are capable of modulating different types of cellular responses including, viability, adhesion and morphology. The results showed a preferential adhesion of both cell types on the microstructured surfaces compared to the unstructured ones. In particular, the fibroblast NIH/3T3 cells show optimal adhesion for small roughness ratios, independent of the surface wettability and polymer type, indicating a non-monotonic dependence of cell adhesion on surface energy. In contrast, the PC12 cells were observed to adhere well to the patterned surfaces independent of the roughness ratio and wettability. These experimental findings are correlated with micromechanical measurements performed on the unstructured and replicated surfaces and discussed on the basis of previous observations describing the relation of cell response to surface energy and rigidity.

  16. CEBAF control system

    International Nuclear Information System (INIS)

    Bork, R.; Grubb, C.; Lahti, G.; Navarro, E.; Sage, J.

    1989-01-01

    A logic-based computer control system is in development at CEBAF. This Unix/C language software package, running on a distributed, hierarchical system of workstation and supervisory minicomputers, interfaces to hardware via CAMAC. Software aspects to be covered are ladder logic, interactive database generation, networking, and graphic user interfaces. 1 fig

  17. PSR control system

    International Nuclear Information System (INIS)

    Clout, P.N.; Conley, A.P.; Bair, S.S.; Fuka, M.A.; Sandford, E.L.; Lander, R.F.; Wells, F.D.

    1985-01-01

    The control system for the Proton Storage Ring at Los Alamos has been essentially completed. Modifications are being applied that are required in the light of machine physics and operating experience. The present design of the system is described and future planned modifications are indicated

  18. Environment control system

    International Nuclear Information System (INIS)

    Sammarone, D.G.

    1978-01-01

    Disclosed is a system for controlling the environment of an enclosed area in nuclear reactor installations. The system permits the changing of the environment from nitrogen to air, or from air to nitrogen, without the release of any radioactivity or process gas to the outside atmosphere

  19. The ISOLDE control system

    Science.gov (United States)

    Deloose, I.; Pace, A.

    1994-12-01

    The two CERN isotope separators named ISOLDE have been running on the new Personal Computer (PC) based control system since April 1992. The new architecture that makes heavy use of the commercial software and hardware of the PC market has been implemented on the 1700 geographically distributed control channels of the two separators and their experimental area. Eleven MSDOS Intel-based PCs with approximately 80 acquisition and control boards are used to access the equipment and are controlled from three PCs running Microsoft Windows used as consoles through a Novell Local Area Network. This paper describes the interesting solutions found and discusses the reduced programming workload and costs that have been obtained.

  20. The value of repeating studies and multiple controls: replicated 28-day growth studies of rainbow trout exposed to clofibric acid.

    Science.gov (United States)

    Owen, Stewart F; Huggett, Duane B; Hutchinson, Thomas H; Hetheridge, Malcolm J; McCormack, Paul; Kinter, Lewis B; Ericson, Jon F; Constantine, Lisa A; Sumpter, John P

    2010-12-01

    Two studies to examine the effect of waterborne clofibric acid (CA) on growth-rate and condition of rainbow trout were conducted using accepted regulatory tests (Organisation for Economic Co-operation and Development [OECD] 215). The first study (in 2005) showed significant reductions after 21 d of exposure (21-d growth lowest-observed-effect concentration [LOEC] = 0.1 µg/L, 21-d condition LOEC = 0.1 µg/L) that continued to 28 d. Growth rate was reduced by approximately 50% (from 5.27 to 2.67% per day), while the condition of the fish reduced in a concentration-dependant manner. Additionally, in a concentration-dependent manner, significant changes in relative liver size were observed, such that increasing concentrations of CA resulted in smaller livers after 28-d exposure. A no-observed-effect concentration (NOEC) was not achieved in the 2005 study. An expanded second study (in 2006) that included a robust bridge to the 2005 study, with four replicate tanks of eight individual fish per concentration, did not repeat the 2005 findings. In the 2006 study, no significant effect on growth rate, condition, or liver biometry was observed after 21 or 28 d (28-d growth NOEC = 10 µg/L, 28-d condition NOEC = 10 µg/L), contrary to the 2005 findings. We do not dismiss either of these findings and suggest both are relevant and stand for comparison. However, the larger 2006 study carries more statistical power and multiple-tank replication, so probably produced the more robust findings. Despite sufficient statistical power in each study, interpretation of these and similar studies should be conducted with caution, because much significance is placed on the role of limited numbers of individual and tank replicates and the influence of control animals. Copyright © 2010 SETAC.

  1. A Gossip-Based Optimistic Replication for Efficient Delay-Sensitive Streaming Using an Interactive Middleware Support System

    Science.gov (United States)

    Mavromoustakis, Constandinos X.; Karatza, Helen D.

    2010-06-01

    While sharing resources the efficiency is substantially degraded as a result of the scarceness of availability of the requested resources in a multiclient support manner. These resources are often aggravated by many factors like the temporal constraints for availability or node flooding by the requested replicated file chunks. Thus replicated file chunks should be efficiently disseminated in order to enable resource availability on-demand by the mobile users. This work considers a cross layered middleware support system for efficient delay-sensitive streaming by using each device's connectivity and social interactions in a cross layered manner. The collaborative streaming is achieved through the epidemically replicated file chunk policy which uses a transition-based approach of a chained model of an infectious disease with susceptible, infected, recovered and death states. The Gossip-based stateful model enforces the mobile nodes whether to host a file chunk or not or, when no longer a chunk is needed, to purge it. The proposed model is thoroughly evaluated through experimental simulation taking measures for the effective throughput Eff as a function of the packet loss parameter in contrast with the effectiveness of the replication Gossip-based policy.

  2. Replication and dimensional quality control of industrial nanoscale surfaces using calibrated AFM measurements and SEM image processing

    DEFF Research Database (Denmark)

    Tosello, Guido; Hansen, Hans Nørgaard; Marinello, F.

    2010-01-01

    application of AFM to calibrate height, depth and pitch of sub-micrometer features and SEM image processing to detect replication accuracy in terms of number of replicated features. Surface replication is analyzed using a metrological approach: nano-features on nickel stampers and injection...

  3. TMX magnet control system

    International Nuclear Information System (INIS)

    Goerz, D.A.

    1978-01-01

    A control system utilizing a microcomputer has been developed that controls the power supplies driving the Tandem Mirror Experiment (TMX) magnet set and monitors magnet coil operation. The magnet set consists of 18 magnet coils that are driven by 26 dc power supplies. There are two possible modes of operation with this system: a pulse mode where the coils are pulsed on for several seconds with a dc power consumption of 16 MW; and a continuous mode where the coils can run steady state at 10 percent of maximum current ratings. The processor has been given an active control role and serves as an interface between the operator and electronic circuitry that controls the magnet power supplies. This microcomputer also collects and processes data from many analog singal monitors in the coil circuits and numerous status signals from the supplies. Placing the microcomputer in an active control role has yielded a compact, cost effective system that simplifies the magnet system operation and has proven to be very reliable. This paper will describe the TMX magnet control sytem and discuss its development

  4. An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and function.

    Directory of Open Access Journals (Sweden)

    Ling Liu

    2009-03-01

    Full Text Available Brome mosaic virus (BMV protein 1a has multiple key roles in viral RNA replication. 1a localizes to perinuclear endoplasmic reticulum (ER membranes as a peripheral membrane protein, induces ER membrane invaginations in which RNA replication complexes form, and recruits and stabilizes BMV 2a polymerase (2a(Pol and RNA replication templates at these sites to establish active replication complexes. During replication, 1a provides RNA capping, NTPase and possibly RNA helicase functions. Here we identify in BMV 1a an amphipathic alpha-helix, helix A, and use NMR analysis to define its structure and propensity to insert in hydrophobic membrane-mimicking micelles. We show that helix A is essential for efficient 1a-ER membrane association and normal perinuclear ER localization, and that deletion or mutation of helix A abolishes RNA replication. Strikingly, mutations in helix A give rise to two dramatically opposite 1a function phenotypes, implying that helix A acts as a molecular switch regulating the intricate balance between separable 1a functions. One class of helix A deletions and amino acid substitutions markedly inhibits 1a-membrane association and abolishes ER membrane invagination, viral RNA template recruitment, and replication, but doubles the 1a-mediated increase in 2a(Pol accumulation. The second class of helix A mutations not only maintains efficient 1a-membrane association but also amplifies the number of 1a-induced membrane invaginations 5- to 8-fold and enhances viral RNA template recruitment, while failing to stimulate 2a(Pol accumulation. The results provide new insights into the pathways of RNA replication complex assembly and show that helix A is critical for assembly and function of the viral RNA replication complex, including its central role in targeting replication components and controlling modes of 1a action.

  5. Adiponectin, a downstream target gene of peroxisome proliferator-activated receptor γ, controls hepatitis B virus replication

    International Nuclear Information System (INIS)

    Yoon, Sarah; Jung, Jaesung; Kim, Taeyeung; Park, Sun; Chwae, Yong-Joon; Shin, Ho-Joon; Kim, Kyongmin

    2011-01-01

    In this study, HepG2-hepatitis B virus (HBV)-stable cells that did not overexpress HBx and HBx-deficient mutant-transfected cells were analyzed for their expression of HBV-induced, upregulated adipogenic and lipogenic genes. The mRNAs of CCAAT enhancer binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), adiponectin, liver X receptor α (LXRα), sterol regulatory element binding protein 1c (SREBP1c), and fatty acid synthase (FAS) were expressed at higher levels in HepG2-HBV and lamivudine-treated stable cells and HBx-deficient mutant-transfected cells than in the HepG2 cells. Lamivudine treatment reduced the mRNA levels of PPARγ and C/EBPα. Conversely, HBV replication was upregulated by adiponectin and PPARγ agonist rosiglitazone treatments and was downregulated by adiponectin siRNAs. Collectively, our results demonstrate that HBV replication and/or protein expression, even in the absence of HBx, upregulated adipogenic or lipogenic genes, and that the control of adiponectin might prove useful as a therapeutic modality for the treatment of chronic hepatitis B.

  6. System control and display

    International Nuclear Information System (INIS)

    Jacobs, J.

    1977-01-01

    The system described was designed, developed, and installed on short time scales and primarily utilized of-the-shelf military and commercial hardware. The system was designed to provide security-in-depth and multiple security options with several stages of redundancy. Under normal operating conditions, the system is computer controlled with manual backup during abnormal conditions. Sensor alarm data are processed in conjunction with weather data to reduce nuisance alarms. A structured approach is used to order alarmed sectors for assessment. Alarm and video information is presented to security personnel in an interactive mode. Historical operational data are recorded for system evaluation

  7. Ion implantation control system

    International Nuclear Information System (INIS)

    Gault, R. B.; Keutzer, L. L.

    1985-01-01

    A control system is disclosed for an ion implantation system of the type in which the wafers to be implanted are mounted around the periphery of a disk which rotates and also moves in a radial direction relative to an ion beam to expose successive sections of each wafer to the radiation. The control system senses beam current which passes through one or more apertures in the disk and is collected by a Faraday cup. This current is integrated to obtain a measure of charge which is compared with a calculated value based upon the desired ion dosage and other parameters. The resultant controls the number of incremental steps the rotating disk moves radially to expose the adjacent sections of each wafer. This process is continued usually with two or more traverses until the entire surface of each wafer has been implanted with the proper ion dosage

  8. The TRISTAN control system

    International Nuclear Information System (INIS)

    Kurokawa, Shinichi; Akiyama, Atsuyoshi; Ishii, Kazuhiro; Kadokura, Eiichi; Katoh, Tadahiko; Kawamoto, Takashi; Kikutani, Eiji; Kimura, Yoshitaka; Koiso, Haruyo; Komada, Ichitaka; Kudo, Kikuo; Naito, Takashi; Oide, Katsunobu; Takeda, Shigeru; Uchino, Kenji; Urakawa, Junji; Shinomoto, Manabu; Kurihara, Michio; Abe, Kenichi

    1986-01-01

    The 8 GeV accumulation ring and the 30 GeV main ring of TRISTAN, an accelerator-storage ring complex at KEK, are controlled by a highly computerized control system. Twenty-four minicomputers are linked by optical fiber cables to form an N-to-N token ring network. The transmission speed on the cables is 10 Mbps. From each minicomputer, a CAMAC serial highway extends to the controlled equipment. At present, twenty minicomputers are connected to the network and are used to control the accumulation ring. The software system is based on the NODAL language devised at the CERN SPS. The KEK NODAL system retains main features of the original NODAL: the interpretive scheme, the multi-computer programming facility, and the data-module concept. In addition, it has the following features: (1) fast execution due to the compiler-interpreter method, (2) a multi-computer file system (3), a full-screen editing facility, and (4) a dynamic linkage scheme for data modules and NODAL functions. The accelerators are operated through five operator consoles, each of which is mangaged by one minicomputer in the network. An operator console contains two 20-inch high-resolution color graphic displays, a pair of touch-panels, and ten small TV monitors. One touch-panel is used to select a program and a piece of equipment to be controlled; the other is used mainly to perform the console actions. (orig.)

  9. neural control system

    International Nuclear Information System (INIS)

    Elshazly, A.A.E.

    2002-01-01

    Automatic power stabilization control is the desired objective for any reactor operation , especially, nuclear power plants. A major problem in this area is inevitable gap between a real plant ant the theory of conventional analysis and the synthesis of linear time invariant systems. in particular, the trajectory tracking control of a nonlinear plant is a class of problems in which the classical linear transfer function methods break down because no transfer function can represent the system over the entire operating region . there is a considerable amount of research on the model-inverse approach using feedback linearization technique. however, this method requires a prices plant model to implement the exact linearizing feedback, for nuclear reactor systems, this approach is not an easy task because of the uncertainty in the plant parameters and un-measurable state variables . therefore, artificial neural network (ANN) is used either in self-tuning control or in improving the conventional rule-based exper system.the main objective of this thesis is to suggest an ANN, based self-learning controller structure . this method is capable of on-line reinforcement learning and control for a nuclear reactor with a totally unknown dynamics model. previously, researches are based on back- propagation algorithm . back -propagation (BP), fast back -propagation (FBP), and levenberg-marquardt (LM), algorithms are discussed and compared for reinforcement learning. it is found that, LM algorithm is quite superior

  10. Modes of DNA repair and replication

    International Nuclear Information System (INIS)

    Hanawalt, P.; Kondo, S.

    1979-01-01

    Modes of DNA repair and replication require close coordination as well as some overlap of enzyme functions. Some classes of recovery deficient mutants may have defects in replication rather than repair modes. Lesions such as the pyrimidine dimers produced by ultraviolet light irradiation are the blocks to normal DNA replication in vivo and in vitro. The DNA synthesis by the DNA polymerase 1 of E. coli is blocked at one nucleotide away from the dimerized pyrimidines in template strands. Thus, some DNA polymerases seem to be unable to incorporate nucleotides opposite to the non-pairing lesions in template DNA strands. The lesions in template DNA strands may block the sequential addition of nucleotides in the synthesis of daughter strands. Normal replication utilizes a constitutive ''error-free'' mode that copies DNA templates with high fidelity, but which may be totally blocked at a lesion that obscures the appropriate base pairing specificity. It might be expected that modified replication system exhibits generally high error frequency. The error rate of DNA polymerases may be controlled by the degree of phosphorylation of the enzyme. Inducible SOS system is controlled by recA genes that also control the pathways for recombination. It is possible that SOS system involves some process other than the modification of a blocked replication apparatus to permit error-prone transdimer synthesis. (Yamashita, S.)

  11. RHIC control system

    Energy Technology Data Exchange (ETDEWEB)

    Barton, D.S. E-mail: dsbarton@bnl.gov; Binello, S.; Buxton, W.; Clifford, T.; D' Ottavio, T.; Hartmann, H.; Hoff, L.T.; Katz, R.; Kennell, S.; Kerner, T.; Laster, J.; Lee, R.C.; Marusic, A.; Michnoff, R.; Morris, J.; Oerter, B.R.; Olsen, R.; Piacentino, J.; Skelly, J.F

    2003-03-01

    The RHIC control system architecture is hierarchical and consists of two physical layers with a fiber-optic network connection. The Front-End Level systems consist of VME chassis with processors running a real-time operating system and both VME I/O modules and remote bus interfaces. Accelerator device software interfaces are implemented as objects in C++. The network implementation uses high speed, switched Ethernet technology. Specialized hardware modules were built for waveform control of power supplies, multiplexed signal acquisition, and timing services. The Console Level systems are Unix workstations. A strong emphasis has been given to developing highly reusable, standard software tools for use in building physics and diagnostic application software.

  12. RHIC control system

    International Nuclear Information System (INIS)

    Barton, D.S.; Binello, S.; Buxton, W.; Clifford, T.; D'Ottavio, T.; Hartmann, H.; Hoff, L.T.; Katz, R.; Kennell, S.; Kerner, T.; Laster, J.; Lee, R.C.; Marusic, A.; Michnoff, R.; Morris, J.; Oerter, B.R.; Olsen, R.; Piacentino, J.; Skelly, J.F.

    2003-01-01

    The RHIC control system architecture is hierarchical and consists of two physical layers with a fiber-optic network connection. The Front-End Level systems consist of VME chassis with processors running a real-time operating system and both VME I/O modules and remote bus interfaces. Accelerator device software interfaces are implemented as objects in C++. The network implementation uses high speed, switched Ethernet technology. Specialized hardware modules were built for waveform control of power supplies, multiplexed signal acquisition, and timing services. The Console Level systems are Unix workstations. A strong emphasis has been given to developing highly reusable, standard software tools for use in building physics and diagnostic application software

  13. ZEUS hardware control system

    Science.gov (United States)

    Loveless, R.; Erhard, P.; Ficenec, J.; Gather, K.; Heath, G.; Iacovacci, M.; Kehres, J.; Mobayyen, M.; Notz, D.; Orr, R.; Orr, R.; Sephton, A.; Stroili, R.; Tokushuku, K.; Vogel, W.; Whitmore, J.; Wiggers, L.

    1989-12-01

    The ZEUS collaboration is building a system to monitor, control and document the hardware of the ZEUS detector. This system is based on a network of VAX computers and microprocessors connected via ethernet. The database for the hardware values will be ADAMO tables; the ethernet connection will be DECNET, TCP/IP, or RPC. Most of the documentation will also be kept in ADAMO tables for easy access by users.

  14. ZEUS hardware control system

    International Nuclear Information System (INIS)

    Loveless, R.; Erhard, P.; Ficenec, J.; Gather, K.; Heath, G.; Iacovacci, M.; Kehres, J.; Mobayyen, M.; Notz, D.; Orr, R.; Sephton, A.; Stroili, R.; Tokushuku, K.; Vogel, W.; Whitmore, J.; Wiggers, L.

    1989-01-01

    The ZEUS collaboration is building a system to monitor, control and document the hardware of the ZEUS detector. This system is based on a network of VAX computers and microprocessors connected via ethernet. The database for the hardware values will be ADAMO tables; the ethernet connection will be DECNET, TCP/IP, or RPC. Most of the documentation will also be kept in ADAMO tables for easy access by users. (orig.)

  15. Effector-Triggered Self-Replication in Coupled Subsystems.

    Science.gov (United States)

    Komáromy, Dávid; Tezcan, Meniz; Schaeffer, Gaël; Marić, Ivana; Otto, Sijbren

    2017-11-13

    In living systems processes like genome duplication and cell division are carefully synchronized through subsystem coupling. If we are to create life de novo, similar control over essential processes such as self-replication need to be developed. Here we report that coupling two dynamic combinatorial subsystems, featuring two separate building blocks, enables effector-mediated control over self-replication. The subsystem based on the first building block shows only self-replication, whereas that based on the second one is solely responsive toward a specific external effector molecule. Mixing the subsystems arrests replication until the effector molecule is added, resulting in the formation of a host-effector complex and the liberation of the building block that subsequently engages in self-replication. The onset, rate and extent of self-replication is controlled by the amount of effector present. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Control of complex systems

    CERN Document Server

    Albertos, Pedro; Blanke, Mogens; Isidori, Alberto; Schaufelberger, Walter; Sanz, Ricardo

    2001-01-01

    The world of artificial systems is reaching complexity levels that es­ cape human understanding. Surface traffic, electricity distribution, air­ planes, mobile communications, etc. , are examples that demonstrate that we are running into problems that are beyond classical scientific or engi­ neering knowledge. There is an ongoing world-wide effort to understand these systems and develop models that can capture its behavior. The reason for this work is clear, if our lack of understanding deepens, we will lose our capability to control these systems and make they behave as we want. Researchers from many different fields are trying to understand and develop theories for complex man-made systems. This book presents re­ search from the perspective of control and systems theory. The book has grown out of activities in the research program Control of Complex Systems (COSY). The program has been sponsored by the Eu­ ropean Science Foundation (ESF) which for 25 years has been one of the leading players in stimula...

  17. Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition

    DEFF Research Database (Denmark)

    Khoudoli, Guennadi A; Gillespie, Peter J; Stewart, Graeme

    2008-01-01

    Although the replication, expression, and maintenance of DNA are well-studied processes, the way that they are coordinated is poorly understood. Here, we report an analysis of the changing association of proteins with chromatin (the chromatin proteome) during progression through interphase...... of the cell cycle. Sperm nuclei were incubated in Xenopus egg extracts, and chromatin-associated proteins were analyzed by mass spectrometry at different times. Approximately 75% of the proteins varied in abundance on chromatin by more than 15%, suggesting that the chromatin proteome is highly dynamic....... Proteins were then assigned to one of 12 different clusters on the basis of their pattern of chromatin association. Each cluster contained functional groups of proteins involved in different nuclear processes related to progression through interphase. We also blocked DNA replication by inhibiting either...

  18. An Analysis of Weakly Consistent Replication Systems in an Active Distributed Network

    OpenAIRE

    Amit Chougule; Pravin Ghewari

    2011-01-01

    With the sudden increase in heterogeneity and distribution of data in wide-area networks, more flexible, efficient and autonomous approaches for management and data distribution are needed. In recent years, the proliferation of inter-networks and distributed applications has increased the demand for geographically-distributed replicated databases. The architecture of Bayou provides features that address the needs of database storage of world-wide applications. Key is the use of weak consisten...

  19. HESYRL control system status

    International Nuclear Information System (INIS)

    Yao Chihyuan

    1992-01-01

    HESYRL synchrotron radiation storage ring was completed in 1989 and has been in commissioning since then. Now it has met its design specification and is ready for synchrotron light experiments. Control system of the project was completed in 1989 and some modifications were made during commissioning. This paper describes its present configuration, status and upgrading plan. (author)

  20. Fault Tolerant Control Systems

    DEFF Research Database (Denmark)

    Bøgh, S. A.

    This thesis considered the development of fault tolerant control systems. The focus was on the category of automated processes that do not necessarily comprise a high number of identical sensors and actuators to maintain safe operation, but still have a potential for improving immunity to component...

  1. Lighting Control Systems Handbook.

    Science.gov (United States)

    1985-06-01

    cost, both initial and operating. Initially, the control system designer must collect in- formation and then study and weigh several areas including...8217odLe 045. Pearl Harbor. III: Code 11 Pearl Harbor ar ho I ir I L ’ odk 402. R IYI& [’. Plearl II arbor I II: Li bra ry. Pearl HaIitrbor. I ai

  2. GLCTA control system

    International Nuclear Information System (INIS)

    Terunuma, N.; Hayano, H.; Higo, T.; Saeki, T.; Suehara, T.; Watanabe, K.

    2004-01-01

    Research and development for the high power X-band RF technologies have been performed on the GLC Test Accelerator, GLCTA, since fall of 2003. The control system of this facility is based on the PC-Linux servers that handle the CAMAC, VME and PLC modules. Automated RF processing and data accumulation of the RF breakdown have been performed. (author)

  3. Multi-dose Romidepsin Reactivates Replication Competent SIV in Post-antiretroviral Rhesus Macaque Controllers.

    Directory of Open Access Journals (Sweden)

    Benjamin B Policicchio

    2016-09-01

    Full Text Available Viruses that persist despite seemingly effective antiretroviral treatment (ART and can reinitiate infection if treatment is stopped preclude definitive treatment of HIV-1 infected individuals, requiring lifelong ART. Among strategies proposed for targeting these viral reservoirs, the premise of the "shock and kill" strategy is to induce expression of latent proviruses [for example with histone deacetylase inhibitors (HDACis] resulting in elimination of the affected cells through viral cytolysis or immune clearance mechanisms. Yet, ex vivo studies reported that HDACis have variable efficacy for reactivating latent proviruses, and hinder immune functions. We developed a nonhuman primate model of post-treatment control of SIV through early and prolonged administration of ART and performed in vivo reactivation experiments in controller RMs, evaluating the ability of the HDACi romidepsin (RMD to reactivate SIV and the impact of RMD treatment on SIV-specific T cell responses. Ten RMs were IV-infected with a SIVsmmFTq transmitted-founder infectious molecular clone. Four RMs received conventional ART for >9 months, starting from 65 days post-infection. SIVsmmFTq plasma viremia was robustly controlled to <10 SIV RNA copies/mL with ART, without viral blips. At ART cessation, initial rebound viremia to ~106 copies/mL was followed by a decline to < 10 copies/mL, suggesting effective immune control. Three post-treatment controller RMs received three doses of RMD every 35-50 days, followed by in vivo experimental depletion of CD8+ cells using monoclonal antibody M-T807R1. RMD was well-tolerated and resulted in a rapid and massive surge in T cell activation, as well as significant virus rebounds (~104 copies/ml peaking at 5-12 days post-treatment. CD8+ cell depletion resulted in a more robust viral rebound (107 copies/ml that was controlled upon CD8+ T cell recovery. Our results show that RMD can reactivate SIV in vivo in the setting of post-ART viral control

  4. Internet Congestion Control System

    Directory of Open Access Journals (Sweden)

    Pranoto Rusmin

    2010-10-01

    Full Text Available Internet congestion occurs when resource demands exceeds the network capacity. But, it is not the only reason. Congestion can happen on some users because some others user has higher sending rate. Then some users with lower sending rate will experience congestion. This partial congestion is caused by inexactly feedback. At this moment congestion are solved by the involvement of two controlling mechanisms. These mechanisms are flow/congestion control in the TCP source and Active Queue Management (AQM in the router. AQM will provide feedback to the source a kind of indication for the occurrence of the congestion in the router, whereas the source will adapt the sending rate appropriate with the feedback. These mechanisms are not enough to solve internet congestion problem completely. Therefore, this paper will explain internet congestion causes, weakness, and congestion control technique that researchers have been developed. To describe congestion system mechanisms and responses, the system will be simulated by Matlab.

  5. PEP computer control system

    International Nuclear Information System (INIS)

    1979-03-01

    This paper describes the design and performance of the computer system that will be used to control and monitor the PEP storage ring. Since the design is essentially complete and much of the system is operational, the system is described as it is expected to 1979. Section 1 of the paper describes the system hardware which includes the computer network, the CAMAC data I/O system, and the operator control consoles. Section 2 describes a collection of routines that provide general services to applications programs. These services include a graphics package, data base and data I/O programs, and a director programm for use in operator communication. Section 3 describes a collection of automatic and semi-automatic control programs, known as SCORE, that contain mathematical models of the ring lattice and are used to determine in real-time stable paths for changing beam configuration and energy and for orbit correction. Section 4 describes a collection of programs, known as CALI, that are used for calibration of ring elements

  6. The ISOLDE control system

    International Nuclear Information System (INIS)

    Deloose, I.; Pace, A.

    1994-01-01

    The two CERN isotope separators named ISOLDE have been running on the new Personal Computer (PC) based control system since April 1992. The new architecture that makes heavy use of the commercial software and hardware of the PC market has been implemented on the 1700 geographically distributed control channels of the two separators and their experimental area. Eleven MSDOS Intel-based PCs with approximately 80 acquisition and control boards are used to access the equipment and are controlled from three PCs running Microsoft Windows used as consoles through a Novell Local Area Network. This paper describes the interesting solutions found and discusses the reduced programming workload and costs that have been obtained. ((orig.))

  7. Vaccination with Gag, Vif, and Nef gene fragments affords partial control of viral replication after mucosal challenge with SIVmac239.

    Science.gov (United States)

    Martins, Mauricio A; Wilson, Nancy A; Piaskowski, Shari M; Weisgrau, Kim L; Furlott, Jessica R; Bonaldo, Myrna C; Veloso de Santana, Marlon G; Rudersdorf, Richard A; Rakasz, Eva G; Keating, Karen D; Chiuchiolo, Maria J; Piatak, Michael; Allison, David B; Parks, Christopher L; Galler, Ricardo; Lifson, Jeffrey D; Watkins, David I

    2014-07-01

    Broadly targeted cellular immune responses are thought to be important for controlling replication of human and simian immunodeficiency viruses (HIV and SIV). However, eliciting such responses by vaccination is complicated by immunodominance, the preferential targeting of only a few of the many possible epitopes of a given antigen. This phenomenon may be due to the coexpression of dominant and subdominant epitopes by the same antigen-presenting cell and may be overcome by distributing these sequences among several different vaccine constructs. Accordingly, we tested whether vaccinating rhesus macaques with "minigenes" encoding fragments of Gag, Vif, and Nef resulted in broadened cellular responses capable of controlling SIV replication. We delivered these minigenes through combinations of recombinant Mycobacterium bovis BCG (rBCG), electroporated recombinant DNA (rDNA) along with an interleukin-12 (IL-12)-expressing plasmid (EP rDNA plus pIL-12), yellow fever vaccine virus 17D (rYF17D), and recombinant adenovirus serotype 5 (rAd5). Although priming with EP rDNA plus pIL-12 increased the breadth of vaccine-induced T-cell responses, this effect was likely due to the improved antigen delivery afforded by electroporation rather than modulation of immunodominance. Indeed, Mamu-A*01(+) vaccinees mounted CD8(+) T cells directed against only one subdominant epitope, regardless of the vaccination regimen. After challenge with SIVmac239, vaccine efficacy was limited to a modest reduction in set point in some of the groups and did not correlate with standard T-cell measurements. These findings suggest that broad T-cell responses elicited by conventional vectors may not be sufficient to substantially contain AIDS virus replication. Immunodominance poses a major obstacle to the generation of broadly targeted, HIV-specific cellular responses by vaccination. Here we attempted to circumvent this phenomenon and thereby broaden the repertoire of SIV-specific cellular responses by

  8. Perceptions of control, burnout, and depressive symptomatology: a replication and extension.

    Science.gov (United States)

    McKnight, J D; Glass, D C

    1995-06-01

    In this 2-year follow-up of 100 nurses, significant increases were observed for burnout and depressive symptomatology. Analysis of change scores and structural equations suggested that the variance shared by burnout and depression (20%) may be attributable to their co-development. A definitive temporal sequence among measures of burnout and depressive affect was not obtained. At initial assessment and follow-up, burned-out nurses displayed accurate perceptions of job uncontrollability, whereas non-burned-out nurses overestimated job control. Perceptual accuracy increased in linear fashion with degree of burnout, irrespective of depressive symptomatology. Frequency of threats to job control predicted a significant amount of the variance in perceptual accuracy, supporting the view that "burnout realism" is reality driven.

  9. Dynamitron control systems

    International Nuclear Information System (INIS)

    Lisanti, Thomas F.

    2005-01-01

    The Dynamitron control system utilizes the latest personal computer technology in control circuitry and components. Both the DPC-2000 and newer Millennium series of control systems make use of their modular architecture in both software and hardware to keep up with customer and engineering demands. This also allows the main structure of the software to remain constant for the user while software drivers are easily changed as hardware demands are modified and improved. The system is presented as four units; the Remote I/O (Input/Output), Local Analog and Digital I/O, Operator Interface and the Main Computer. The operator is provided with a selection of many informative screen displays. The control program handles all graphic screen displays and the updating of these screens directly; it does not communicate to a display terminal. This adds to the quick response and excellent operator feedback received while operating the accelerator. The CPU also has the ability to store and record all process variable setpoints for each product that will be treated. All process parameters are printed to a report at regular intervals during a process run for record keeping

  10. Wireless Remote Control System

    Directory of Open Access Journals (Sweden)

    Adrian Tigauan

    2012-06-01

    Full Text Available This paper presents the design of a wireless remote control system based on the ZigBee communication protocol. Gathering data from sensors or performing control tasks through wireless communication is advantageous in situations in which the use of cables is impractical. An Atmega328 microcontroller (from slave device is used for gathering data from the sensors and transmitting it to a coordinator device with the help of the XBee modules. The ZigBee standard is suitable for low-cost, low-data-rate and low-power wireless networks implementations. The XBee-PRO module, designed to meet ZigBee standards, requires minimal power for reliable data exchange between devices over a distance of up to 1600m outdoors. A key component of the ZigBee protocol is the ability to support networking and this can be used in a wireless remote control system. This system may be employed e.g. to control temperature and humidity (SHT11 sensor and light intensity (TSL230 sensor levels inside a commercial greenhouse.

  11. Management control system description

    Energy Technology Data Exchange (ETDEWEB)

    Bence, P. J.

    1990-10-01

    This Management Control System (MCS) description describes the processes used to manage the cost and schedule of work performed by Westinghouse Hanford Company (Westinghouse Hanford) for the US Department of Energy, Richland Operations Office (DOE-RL), Richland, Washington. Westinghouse Hanford will maintain and use formal cost and schedule management control systems, as presented in this document, in performing work for the DOE-RL. This MCS description is a controlled document and will be modified or updated as required. This document must be approved by the DOE-RL; thereafter, any significant change will require DOE-RL concurrence. Westinghouse Hanford is the DOE-RL operations and engineering contractor at the Hanford Site. Activities associated with this contract (DE-AC06-87RL10930) include operating existing plant facilities, managing defined projects and programs, and planning future enhancements. This document is designed to comply with Section I-13 of the contract by providing a description of Westinghouse Hanford's cost and schedule control systems used in managing the above activities. 5 refs., 22 figs., 1 tab.

  12. Cyclin-dependent kinase suppression by WEE1 kinase protects the genome through control of replication initiation and nucleotide consumption

    DEFF Research Database (Denmark)

    Beck, Halfdan; Nähse-Kumpf, Viola; Larsen, Marie Sofie Yoo

    2012-01-01

    Activation of oncogenes or inhibition of WEE1 kinase deregulates Cyclin-dependent kinase (CDK) activity and leads to replication stress, however, the underlying mechanism is not understood. We now show that elevation of CDK activity by inhibiting WEE1 kinase rapidly increases initiation of replic......Activation of oncogenes or inhibition of WEE1 kinase deregulates Cyclin-dependent kinase (CDK) activity and leads to replication stress, however, the underlying mechanism is not understood. We now show that elevation of CDK activity by inhibiting WEE1 kinase rapidly increases initiation...... of replication. This leads to nucleotide shortage and reduces replication fork speed, which is followed by SLX4/MUS81-mediated DNA double-strand breakage. Fork speed is normalized and DNA double-strand break (DSB) formation is suppressed when CDT1, a key factor for replication initiation, is depleted...

  13. Performance-Based Scholarships: Replication at Six Sites Using Randomized Controlled Trials

    Science.gov (United States)

    Mayer, Alexander

    2014-01-01

    Performance-based scholarships were developed to help tackle some of the financial obstacles facing students in the postsecondary education system. In general, these scholarships aim to help reduce the financial burdens of low-income college students, and are structured to help incentivize good academic progress. Performance-based scholarships…

  14. Access control system operation

    International Nuclear Information System (INIS)

    Barnes, L.D.

    1981-06-01

    An automated method for the control and monitoring of personnel movement throughout the site was developed under contract to the Department of Energy by Allied-General Nuclear Services (AGNS) at the Barnwell Nuclear Fuel Plant (BNFP). These automated features provide strict enforcement of personnel access policy without routine patrol officer involvement. Identification methods include identification by employee ID number, identification by voice verification and identification by physical security officer verification. The ability to grant each level of access authority is distributed over the organization to prevent any single individual at any level in the organization from being capable of issuing an authorization for entry into sensitive areas. Each access event is recorded. As access events occur, the inventory of both the entered and the exited control area is updated so that a current inventory is always available for display. The system has been operated since 1979 in a development mode and many revisions have been implemented in hardware and software as areas were added to the system. Recent changes have involved the installation of backup systems and other features required to achieve a high reliability. The access control system and recent operating experience are described

  15. The COSY control system

    International Nuclear Information System (INIS)

    Bongers, N.; Hacker, U.; Henn, K.; Richert, A.; Simon, M.; Sobotta, K.; Stephan, M.; Vashegyi, T.; Weinert, A.

    1992-01-01

    The COSY control system architecture is organized strongly hierarchically with distributed intelligence and extensive use of standards. At the top level of computer control hardware work stations give the operator graphical access to the process. For these tasks Hewlett Packard HP 9000 Series 700 computers with HP-UX and X-Windows/Motif are in use. Also used as work-cells this RISC computers give computing power for model calculations and long term databases. This computers are interconnected using Ethernet and TCP/IP to the next layer of hardware. (author) 3 refs.; 5 figs

  16. Mechanisms of DNA replication termination.

    Science.gov (United States)

    Dewar, James M; Walter, Johannes C

    2017-08-01

    Genome duplication is carried out by pairs of replication forks that assemble at origins of replication and then move in opposite directions. DNA replication ends when converging replication forks meet. During this process, which is known as replication termination, DNA synthesis is completed, the replication machinery is disassembled and daughter molecules are resolved. In this Review, we outline the steps that are likely to be common to replication termination in most organisms, namely, fork convergence, synthesis completion, replisome disassembly and decatenation. We briefly review the mechanism of termination in the bacterium Escherichia coli and in simian virus 40 (SV40) and also focus on recent advances in eukaryotic replication termination. In particular, we discuss the recently discovered E3 ubiquitin ligases that control replisome disassembly in yeast and higher eukaryotes, and how their activity is regulated to avoid genome instability.

  17. Replication of prostate cancer risk loci in a Japanese case-control association study.

    Science.gov (United States)

    Yamada, Hiroki; Penney, Kathryn L; Takahashi, Hiroyuki; Katoh, Takahiko; Yamano, Yuko; Yamakado, Minoru; Kimura, Takahiro; Kuruma, Hidetoshi; Kamata, Yuko; Egawa, Shin; Freedman, Matthew L

    2009-10-07

    Two prostate cancer genome-wide scans in populations of European ancestry identified several genetic variants that are strongly associated with prostate cancer risk. The effect of these risk variants and their cumulative effect in other populations are unknown. We evaluated the association of 23 risk single-nucleotide polymorphisms (SNPs) with prostate cancer risk and clinical covariates (Gleason score, tumor aggressiveness, and age at diagnosis) in men of Japanese ancestry (311 case subjects and 1035 control subjects) using unconditional logistic regression. We also used logistic regression to test the association between increasing numbers of independently associated risk alleles and the risk of prostate cancer, prostate cancer aggressiveness, and age at diagnosis. All statistical tests were two-sided. Seven of the 23 SNPs (five independent loci) were associated with prostate cancer risk (P values ranged from .0084 to 2.3 x 10(-8) and effect sizes [estimated as odds ratios, ORs] ranged from 1.35 to 1.82). None of the seven SNPs was associated with Gleason score or aggressive disease. rs6983561 and rs4430796 were associated with age at diagnosis (Ps = .0188 and .0339, respectively). Men with six or more risk alleles (27% of case patients and 11% of control subjects) had a higher risk of prostate cancer than men with two or fewer risk alleles (7% of case patients and 20% of control subjects) (OR = 6.22, P = 1.5 x 10(-12)). These results highlight the critical importance of considering ancestry in understanding how risk alleles influence disease and suggest that risk estimates and variants differ across populations. It is important to perform studies in multiple ancestral populations so that the composite genetic architecture of prostate cancer can be rigorously addressed.

  18. Negative transfer of heart rate control following biofeedback training: a partial replication.

    Science.gov (United States)

    Steptoe, A; Macready, D

    1985-09-01

    Ability to raise and lower heart rate (HR) on instruction was tested before and after unidirectional biofeedback training in two groups of 10 male volunteers. Instructional control was assessed in 2-min trials before training, and after 5 and 10 biofeedback trials of increasing (Group I) and decreasing (Group D) HR. The magnitude of HR elevations produced by Group D diminished following training, while modifications in Group I were unchanged. This negative transfer effect is discussed in relation to whether voluntary speeding and slowing HR reflect distinct capacities.

  19. The UNK control system

    International Nuclear Information System (INIS)

    Alferov, V.N.; Brook, V.L.; Dunaitsev, A.F.

    1992-01-01

    The IHEP proton Accelerating and Storage Complex (UNK) includes in its first stage a 400 GeV conventional and a 3000 GeV superconducting ring placed in the same underground tunnel of 20.7 km circumference. The beam will be injected into UNK from the existing 70 GeV accelerator U-70. The experimental programme which is planned to start in 1995, will include 3000 GeV fixed target and 400 + 3000 GeV colliding beams physics. The size and complexity of the UNK dictate a distributed multiprocessor architecture of the control system. About 4000 of 8/16 bit controllers, directly attached to the UNK equipment will perform low level control and data acquisition tasks. The equipment controllers will be connected via the MIL-1553 field bus to VME based 32-bit front end computers. The TCP/IP network will interconnect front end computers in the UNK equipment buildings with UNIX workstations and servers in the Main Control Room. The report presents the general architecture and current status of the UNK control. (author)

  20. Automatic heating control system

    Energy Technology Data Exchange (ETDEWEB)

    Whittle, A.J.

    1989-11-15

    A heating control system for buildings comprises at least one heater incorporating heat storage means, a first sensor for detecting temperature within the building, means for setting a demand temperature, a second sensor for detecting outside temperature, a timer, and means for determining the switch on time of the heat storage means on the basis of the demand temperature and the internal and external temperatures. The system may additionally base the switch on time of the storage heater(s) on the heating and cooling rates of the building (as determined from the sensed temperatures); or on the anticipated daytime temperature (determined from the sensed night time temperature). (author).

  1. Intelligent Lighting Control System

    OpenAIRE

    García, Elena; Rodríguez González, Sara; de Paz Santana, Juan F.; Bajo Pérez, Javier

    2014-01-01

    This paper presents an adaptive architecture that allows centralized control of public lighting and intelligent management, in order to economise on lighting and maintain maximum comfort status of the illuminated areas. To carry out this management, architecture merges various techniques of artificial intelligence (AI) and statistics such as artificial neural networks (ANN), multi-agent systems (MAS), EM algorithm, methods based on ANOVA and a Service Oriented Aproach (SOA). It performs optim...

  2. Solving the Blood-Brain Barrier Challenge for the Effective Treatment of HIV Replication in the Central Nervous System.

    Science.gov (United States)

    Bertrand, Luc; Nair, Madhavan; Toborek, Michal

    2016-01-01

    Recent decades mark a great progress in the treatment of HIV infection. What was once a deadly disease is now a chronic infection. However, HIV-infected patients are prone to develop comorbidities, which severely affect their daily functions. For example, a large population of patients develop a variety of neurological and cognitive complications, called HIV associated neurological disorders (HAND). Despite efficient repression of viral replication in the periphery, evidence shows that the virus can remain active in the central nervous system (CNS). This low level of replication is believed to result in a progression of neurocognitive dysfunction in infected individuals. Insufficient viral inhibition in the brain results from the inability of several treatment drugs in crossing the blood-brain barrier (BBB) and reaching therapeutic concentrations in the CNS. The current manuscript discusses several strategies that are being developed to enable therapeutics to cross the BBB, including bypassing BBB, inhibition of efflux transporters, the use of active transporters present at the BBB, and nanotechnology. The increased concentration of therapeutics in the CNS is desirable to prevent viral replication; however, potential side effects of anti-retroviral drugs need also to be taken into consideration.

  3. Controlling chaotic systems via nonlinear feedback control

    International Nuclear Information System (INIS)

    Park, Ju H.

    2005-01-01

    In this article, a new method to control chaotic systems is proposed. Using Lyapunov method, we design a nonlinear feedback controller to make the controlled system be stabilized. A numerical example is given to illuminate the design procedure and advantage of the result derived

  4. Rift valley fever virus nonstructural protein NSs promotes viral RNA replication and transcription in a minigenome system.

    Science.gov (United States)

    Ikegami, Tetsuro; Peters, C J; Makino, Shinji

    2005-05-01

    Rift Valley fever virus (RVFV), which belongs to the genus Phlebovirus, family Bunyaviridae, has a tripartite negative-strand genome (S, M, and L segments) and is an important mosquito-borne pathogen for domestic animals and humans. We established an RVFV T7 RNA polymerase-driven minigenome system in which T7 RNA polymerase from an expression plasmid drove expression of RNA transcripts for viral proteins and minigenome RNA transcripts carrying a reporter gene between both termini of the M RNA segment in 293T cells. Like other viruses of the Bunyaviridae family, replication and transcription of the RVFV minigenome required expression of viral N and L proteins. Unexpectedly, the coexpression of an RVFV nonstructural protein, NSs, with N and L proteins resulted in a significant enhancement of minigenome RNA replication. Coexpression of NSs protein with N and L proteins also enhanced minigenome mRNA transcription in the cells expressing viral-sense minigenome RNA transcripts. NSs protein expression increased the RNA replication of minigenomes that originated from S and L RNA segments. Enhancement of minigenome RNA synthesis by NSs protein occurred in cells lacking alpha/beta interferon (IFN-alpha/beta) genes, indicating that the effect of NSs protein on minigenome RNA replication was unrelated to a putative NSs protein-induced inhibition of IFN-alpha/beta production. Our finding that RVFV NSs protein augmented minigenome RNA synthesis was in sharp contrast to reports that Bunyamwera virus (genus Bunyavirus) NSs protein inhibits viral minigenome RNA synthesis, suggesting that RVFV NSs protein and Bunyamwera virus NSs protein have distinctly different biological roles in viral RNA synthesis.

  5. Alzheimer's Disease Sequencing Project discovery and replication criteria for cases and controls: Data from a community-based prospective cohort study with autopsy follow-up.

    Science.gov (United States)

    Crane, Paul K; Foroud, Tatiana; Montine, Thomas J; Larson, Eric B

    2017-12-01

    The Alzheimer's Disease Sequencing Project (ADSP) used different criteria for assigning case and control status from the discovery and replication phases of the project. We considered data from a community-based prospective cohort study with autopsy follow-up where participants could be categorized as case, control, or neither by both definitions and compared the two sets of criteria. We used data from the Adult Changes in Thought (ACT) study including Diagnostic and Statistical Manual-IV criteria for dementia status, McKhann et al. criteria for clinical Alzheimer's disease, and Braak and Consortium to Establish a Registry for AD findings on neurofibrillary tangles and neuritic plaques to categorize the 621 ACT participants of European ancestry who died and came to autopsy. We applied ADSP discovery and replication definitions to identify controls, cases, and people who were neither controls nor cases. There was some agreement between the discovery and replication definitions. Major areas of discrepancy included the finding that only 40% of the discovery sample controls had sufficiently low levels of neurofibrillary tangles and neuritic plaques to be considered controls by the replication criteria and the finding that 16% of the replication phase cases were diagnosed with non-AD dementia during life and thus were excluded as cases for the discovery phase. These findings should inform interpretation of genetic association findings from the ADSP. Differences in genetic association findings between the two phases of the study may reflect these different phenotype definitions from the discovery and replication phase of the ADSP. Copyright © 2017 the Alzheimer's Association. Published by Elsevier Inc. All rights reserved.

  6. Replicative stress and alterations in cell cycle checkpoint controls following acetaminophen hepatotoxicity restrict liver regeneration.

    Science.gov (United States)

    Viswanathan, Preeti; Sharma, Yogeshwar; Gupta, Priya; Gupta, Sanjeev

    2018-03-05

    Acetaminophen hepatotoxicity is a leading cause of hepatic failure with impairments in liver regeneration producing significant mortality. Multiple intracellular events, including oxidative stress, mitochondrial damage, inflammation, etc., signify acetaminophen toxicity, although how these may alter cell cycle controls has been unknown and was studied for its significance in liver regeneration. Assays were performed in HuH-7 human hepatocellular carcinoma cells, primary human hepatocytes and tissue samples from people with acetaminophen-induced acute liver failure. Cellular oxidative stress, DNA damage and cell proliferation events were investigated by mitochondrial membrane potential assays, flow cytometry, fluorescence staining, comet assays and spotted arrays for protein expression after acetaminophen exposures. In experimental groups with acetaminophen toxicity, impaired mitochondrial viability and substantial DNA damage were observed with rapid loss of cells in S and G2/M and cell cycle restrictions or even exit in the remainder. This resulted from altered expression of the DNA damage regulator, ATM and downstream transducers, which imposed G1/S checkpoint arrest, delayed entry into S and restricted G2 transit. Tissues from people with acute liver failure confirmed hepatic DNA damage and cell cycle-related lesions, including restrictions of hepatocytes in aneuploid states. Remarkably, treatment of cells with a cytoprotective cytokine reversed acetaminophen-induced restrictions to restore cycling. Cell cycle lesions following mitochondrial and DNA damage led to failure of hepatic regeneration in acetaminophen toxicity but their reversibility offers molecular targets for treating acute liver failure. © 2018 John Wiley & Sons Ltd.

  7. Replicating Reducing the Risk: 12-Month Impacts of a Cluster Randomized Controlled Trial

    Science.gov (United States)

    Blocklin, Michelle; Layzer, Jean; Price, Cristofer; Juras, Randall; Freiman, Lesley

    2016-01-01

    Objectives. To test the effectiveness of Reducing the Risk, an evidence-based sexual health curriculum designed to help prevent adolescent pregnancy and sexually transmitted infections, on youth sexual behavior and intermediate outcomes thought to lead to these behaviors. Methods. Classes within schools in St. Louis, Missouri; Austin, Texas; and San Diego, California; were randomly assigned to receive Reducing the Risk or “business as usual.” Youths completed Web-based surveys at baseline (preintervention, August 2012–January 2014) and 12 months later (August 2013–January 2015). Intent-to-treat analyses were conducted across sites; we tested for differences in impacts between sites and other subgroups. Results. The program had no overall impact on sexual behaviors. However, at 1 site, program participants were significantly less likely to have engaged in recent sexual intercourse than were control group members. There were positive overall impacts on intermediate outcomes (e.g., knowledge, attitudes). Conclusions. After 12 months, Reducing the Risk was unsuccessful at changing sexual behaviors. Other results were mixed, but promising evidence (e.g., behavioral impacts at 1 site, impacts on intermediate outcomes) suggests potential for more widespread behavioral impacts over a longer term. PMID:27689492

  8. NSLS control system upgrade

    International Nuclear Information System (INIS)

    Smith, J.D.; Ramamoorthy, Susila; Tang, Y.N.

    1994-01-01

    The NSLS consists of two storage rings, a booster and a linac. A major upgrade of the control system (installed in 1978) was undertaken and has been completed. The computer architecture is being changed from a three level star-network to a two level distributed system. The microprocessor subsystem, host computer and workstations, communication link and the main software components are being upgraded or replaced. Since the NSLS rings operate twenty four hours a day a year with minimum maintenance time, the key requirement during the upgrade phase is a non-disruptive transition with minimum downtime. Concurrent with the upgrade, some immediate improvements were required. This paper describes the various components of the upgraded system and outlines the future plans. ((orig.))

  9. NSLS control system upgrade

    International Nuclear Information System (INIS)

    Smith, J.D.; Ramamoorthy, S.; Tang, Yong N.

    1995-01-01

    The NSLS consists of two storage rings, a booster and a linac. A major upgrade of the control system (installed in 1978) was undertaken and has been completed. The computer architecture is being changed from a three level star-network to a two level distributed system. The microprocessor subsystem, host computer and workstations, communication link and the main software components are being upgraded or replaced. Since the NSLS rings operate twenty four hours a day a year with minimum maintenance time, the key requirement during the upgrade phase is a non-disruptive transition with minimum downtime. Concurrent with the upgrade, some immediate improvements were required. This paper describes the various components of the upgraded system and outlines the future plans

  10. Natural controlled HIV infection: Preserved HIV-specific immunity despite undetectable replication competent virus

    International Nuclear Information System (INIS)

    Kloosterboer, Nico; Groeneveld, Paul H.P.; Jansen, Christine A.; Vorst, Teun J.K. van der; Koning, Fransje; Winkel, Carel N.; Duits, Ashley J.; Miedema, Frank; Baarle, Debbie van; Rij, Ronald P. van; Brinkman, Kees; Schuitemaker, Hanneke

    2005-01-01

    Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naive individuals who are strongly seropositive for HIV-1 but who lack evidence of detectable HIV p24 antigen, plasma RNA, and proviral DNA in routine diagnostic testing. With an ultrasensitive PCR, we established that frequencies of pol proviral DNA sequences were as low as 0.2-0.5 copies/10 6 PBMC. HIV could not be isolated using up to 30 x 10 6 patient PBMC. One individual was heterozygous for CCR5 Δ32, but CCR5 expression on CD4 + T cells was normal to high in all four individuals. In vitro R5 and X4 HIV-1 susceptibility of CD8-depleted PBMC of all study subjects was significantly lower than the susceptibility of CD8-depleted PBMC of healthy blood donors. All individuals expressed protective HLA-B*58s alleles and showed evidence of HIV-specific cellular immunity either by staining with HLA-B*57 tetramers folded with an HIV RT or gag peptide or after stimulation with HIV-1 p24 gag, RT, or nef peptides in ELIspot analysis. HIV-specific CD4 + T helper cells were demonstrated by proliferation of CD4 + T cells and intracellular staining for IL-2 and IFNγ after stimulation with an HIV-gag peptide pool. Sera of all individuals showed antibody-mediated neutralization of both R5 and X4 HIV-1 variants. These data implicate that very low-level antigen exposure is sufficient for sustained HIV-specific immunity and suggest the possibility of a multi-factorial control of HIV infection

  11. Incoherent control of locally controllable quantum systems

    International Nuclear Information System (INIS)

    Dong Daoyi; Zhang Chenbin; Rabitz, Herschel; Pechen, Alexander; Tarn, T.-J.

    2008-01-01

    An incoherent control scheme for state control of locally controllable quantum systems is proposed. This scheme includes three steps: (1) amplitude amplification of the initial state by a suitable unitary transformation, (2) projective measurement of the amplified state, and (3) final optimization by a unitary controlled transformation. The first step increases the amplitudes of some desired eigenstates and the corresponding probability of observing these eigenstates, the second step projects, with high probability, the amplified state into a desired eigenstate, and the last step steers this eigenstate into the target state. Within this scheme, two control algorithms are presented for two classes of quantum systems. As an example, the incoherent control scheme is applied to the control of a hydrogen atom by an external field. The results support the suggestion that projective measurements can serve as an effective control and local controllability information can be used to design control laws for quantum systems. Thus, this scheme establishes a subtle connection between control design and controllability analysis of quantum systems and provides an effective engineering approach in controlling quantum systems with partial controllability information.

  12. Heavy metal and waste metabolite accumulation and their affect on rainbow trout performance in a replicated water reuse system operated at low or high system flushing rates

    Science.gov (United States)

    A six-month trial was conducted to compare the effects of high and low make-up water flushing rates on rainbow trout performance and water quality in replicated water reuse aquaculture systems (WRAS). Six identical 9.5 m3 WRAS, containing a single 5.3 m3 tank and operated at a total recirculating fl...

  13. Crawling the Control System

    International Nuclear Information System (INIS)

    Larrieu, Theodore

    2009-01-01

    Information about accelerator operations and the control system resides in various formats in a variety of places on the lab network. There are operating procedures, technical notes, engineering drawings, and other formal controlled documents. There are programmer references and API documentation generated by tools such as doxygen and javadoc. There are the thousands of electronic records generated by and stored in databases and applications such as electronic logbooks, training materials, wikis, and bulletin boards and the contents of text-based configuration files and log files that can also be valuable sources of information. The obvious way to aggregate all these sources is to index them with a search engine that users can then query from a web browser. Toward this end, the Google 'mini' search appliance was selected and implemented because of its low cost and its simple web-based configuration and management. In addition to crawling and indexing electronic documents, the appliance provides an API that has been used to supplement search results with live control system data such as current values of EPICS process variables and graphs of recent data from the archiver.

  14. Radiation control system

    International Nuclear Information System (INIS)

    Murao, Mitsuo.

    1985-01-01

    Purpose: To rapidly and suitably performing planning and designation by radiation-working control systems in the radiation controlled area of nuclear power plant. Method: Various informations regarding radiation exposure are arranged and actual exposure data are statistically stored, to thereby perform forecasting calculation for the radiation exposure upon workings in the plurality of working regions in the radiation controlled area. Based on the forecast values and the registered workers' exposure dose in the past workings are alocated successively such that the total exposure does upon conducting the workings is less than the limited value, to prepare working plans in the areas. Further, procedures for preparing a series of documents regarding the workings in the radiation area are automated to rapidly and properly provide the informations serving to the planning and designation for the radiation workings. As a result, the radiation managers' burnden can be mitigated and an efficient working management system can be provided, in view of the exposure management and personal management. (Kamimura, M.)

  15. Control of optical systems

    Science.gov (United States)

    Founds, D.

    1988-01-01

    Some of the current and planned activities at the Air Force Systems Command in structures and controls for optical-type systems are summarized. Many of the activities are contracted to industry; one task is an in-house program which includes a hardware test program. The objective of the in-house program, referred to as the Aluminum Beam Expander Structure (ABES), is to address issues involved in on-orbit system identification. The structure, which appears similar to the LDR backup structure, is about 35 feet tall. The activity to date has been limited to acquisition of about 250 hours of test data. About 30 hours of data per excitation force is gathered in order to obtain sufficient data for a good statistical estimate of the structural parameters. The development of an Integrated Structural Modeling (ISM) computer program is being done by Boeing Aerospace Company. The objective of the contracted effort is to develop a combined optics, structures, thermal, controls, and multibody dynamics simulation code.

  16. Feedwater control system

    International Nuclear Information System (INIS)

    Cook, B.M.

    1982-01-01

    Excessive swing of the feedwater in nuclear reactor power supply apparatus on the occurrence of a transient is suppressed by injecting an anticipatory compensating signal (δWsub(fw)) into the control for the feedwater. Typical overshoot occurs on removal of a large part of the load, the steam flow is reduced so that the conventional control system reduces the flow of feedwater. At the same time there is a reduction of feedwater level in the steam generator because of the collapse of the bubbles under increased steam pressure. By the time the control responds to the drop in level, the apparatus has begun to stabilize so that there is overshoot. The anticipatory signal is derived from the boiling power (BP) which is a function of the nuclear power (Qsub(N)) developed, the enthalpy of saturated water (hsub(s)) and the enthalpy of the feedwater injected into the steam generator (hsub(fw)). From the boiling power (BP) and the increment in steam pressure resulting from the transient an anticipatory increment of feedwater flow is derived. This increment is added to the other parameters controlling the feedwater. (author)

  17. Phase Control in Nonlinear Systems

    Science.gov (United States)

    Zambrano, Samuel; Seoane, Jesús M.; Mariño, Inés P.; Sanjuán, Miguel A. F.; Meucci, Riccardo

    The following sections are included: * Introduction * Phase Control of Chaos * Description of the model * Numerical exploration of phase control of chaos * Experimental evidence of phase control of chaos * Phase Control of Intermittency in Dynamical Systems * Crisis-induced intermittency and its control * Experimental setup and implementation of the phase control scheme * Phase control of the laser in the pre-crisis regime * Phase control of the intermittency after the crisis * Phase control of the intermittency in the quadratic map * Phase Control of Escapes in Open Dynamical Systems * Control of open dynamical systems * Model description * Numerical simulations and heuristic arguments * Experimental implementation in an electronic circuit * Conclusions and Discussions * Acknowledgments * References

  18. PEP instrumentation and control system

    Energy Technology Data Exchange (ETDEWEB)

    Melen, R.

    1980-06-01

    This paper describes the operating characteristics of the primary components that form the PEP Instrumentation and Control System. Descriptions are provided for the computer control system, beam monitors, and other support systems.

  19. PEP instrumentation and control system

    International Nuclear Information System (INIS)

    Melen, R.

    1980-06-01

    This paper describes the operating characteristics of the primary components that form the PEP Instrumentation and Control System. Descriptions are provided for the computer control system, beam monitors, and other support systems

  20. Environmental Control System Development

    Science.gov (United States)

    Flores Arroyo, Elvin A.

    2018-01-01

    Since before the first men landed on the moon, human beings have aspired to reach farther into space, to discover and answer the great mysteries that exist beyond imagination. To reach where no one has gone before. To able to see all the wonderful things that can be found in space and that only satellites have revealed to us during all this time. Considering the last trip to the moon, mankind has been evolving and improving their technology to reach destinations whose distances had been impossible to transit. To reach that goal, the National Aeronautics and Space Administration (NASA) has designed and developed the largest and most powerful rocket ever created by the human race, the Space Launch System - better known as the SLS. To be able to send this large rocket to space, Kennedy Space Center (KSC) is doing upgrades to their existing facilities and equipment. At Launch Pad 39B, they are setting up a new Environmental Control System (ECS) developed to supply the rocket with the correct gases and mixtures that will be needed for the rocket to launch. The ECS is similar to an air conditioning unit. The main functionality of it is to supply the SLS with the correct gas mixture for it to launch. Also the ECS has been required to reduce or eliminate the possibility of a complete system failure. The system is part of the Ground Support Equipment (GSE) for the SLS that will be going to the Moon and Mars.

  1. Automatically controlled training systems

    International Nuclear Information System (INIS)

    Milashenko, A.; Afanasiev, A.

    1990-01-01

    This paper reports that the computer system for NPP personnel training was developed for training centers in the Soviet Union. The system should be considered as the first step in training, taking into account that further steps are to be devoted to part-task and full scope simulator training. The training room consists of 8-12 IBM PC/AT personal computers combined into a network. A trainee accesses the system in a dialor manner. Software enables the instructor to determine the trainee's progress in different subjects of the program. The quality of any trainee preparedness may be evaluated by Knowledge Control operation. Simplified dynamic models are adopted for separate areas of the program. For example, the system of neutron flux monitoring has a dedicated model. Currently, training, requalification and support of professional qualifications of nuclear power plant operators is being emphasized. A significant number of emergency situations during work are occurring due to operator errors. Based on data from September-October 1989, more than half of all unplanned drops in power and stoppages of power plants were due to operator error. As a comparison, problems due to equipment malfunction accounted for no more than a third of the total. The role of personnel, especially of the operators, is significant during normal operations, since energy production costs as well as losses are influenced by the capability of the staff. These facts all point to the importance of quality training of personnel

  2. A novel minicircle vector based system for inhibting the replication and gene expression of enterovirus 71 and coxsackievirus A16.

    Science.gov (United States)

    Yang, Zhuo; Li, Guodong; Zhang, Yingqiu; Liu, Xiaoman; Tien, Po

    2012-11-01

    Enterovirus 71 (EV 71) and Coxsackievirus A16 (CA 16) are two major causative agents of hand, foot and mouth disease (HFMD). They have been associated with severe neurological and cardiological complications worldwide, and have caused significant mortalities during large-scale outbreaks in China. Currently, there are no effective treatments against EV 71 and CA 16 infections. We now describe the development of a novel minicircle vector based RNA interference (RNAi) system as a therapeutic approach to inhibiting EV 71 and CA 16 replication. Small interfering RNA (siRNA) molecules targeting the conserved regions of the 3C(pro) and 3D(pol) function gene of the EV 71 and CA 16 China strains were designed based on their nucleotide sequences available in GenBank. This RNAi system was found to effectively block the replication and gene expression of these viruses in rhabdomyosarcoma (RD) cells and virus-infected mice model. The inhibitory effects were confirmed by a corresponding decrease in viral RNA, viral protein, and progeny virus production. In addition, no significant adverse off-target silencing or cytotoxic effects were observed. These results demonstrated the potential and feasibility of this novel minicircle vector based RNAi system for antiviral therapy against EV 71 and CA 16 infection. Copyright © 2012 Elsevier B.V. All rights reserved.

  3. Replication and load balancing strategy of STAR's relational database management system (RDBM)

    Energy Technology Data Exchange (ETDEWEB)

    DePhillips, M; Lauret, J; Kopytine, M [Brookhaven National Laboratory, Upton NY 11973 (United States); Kent State University, Kent Ohio 44242 (United States)], E-mail: jlauret@bnl.gov

    2008-07-15

    Database demand resulting from offline analysis and production of data at the STAR experiment at Brookhaven National Laboratory's Relativistic Heavy-Ion Collider has steadily increased over the last six years of data taking activities. With each year, STAR more than doubles the number of events recorded with an anticipation of reaching a billion event capabilities as early as next year. The challenges faced from producing and analyzing this magnitude of events in parallel have raised issues with regard to the distribution of calibrations and geometry data, via databases, to STAR's growing global collaboration. Rapid distribution, availability, ensured synchronization and load balancing have become paramount considerations. Both conventional technology and novel approaches are used in parallel to realize these goals. This paper discusses how STAR uses load balancing to optimize database usage. It discusses distribution methods via MySQL master slave replication; the synchronization issues that arise from this type of distribution and solutions, mostly homegrown, put forth to overcome these issues. A novel approach toward load balancing between slave nodes that assists in maintaining a high availability rate for a veracious community is discussed in detail. This load balancing addresses both, pools of nodes internal to a given location, as well as balancing the load for remote users between different available locations. Challenges, trade-offs, rationale for decisions and paths forward will be discussed in all cases, presenting a solid production environment with a vision for scalable growth.

  4. Replication and load balancing strategy of STAR's relational database management system (RDBM)

    International Nuclear Information System (INIS)

    DePhillips, M; Lauret, J; Kopytine, M

    2008-01-01

    Database demand resulting from offline analysis and production of data at the STAR experiment at Brookhaven National Laboratory's Relativistic Heavy-Ion Collider has steadily increased over the last six years of data taking activities. With each year, STAR more than doubles the number of events recorded with an anticipation of reaching a billion event capabilities as early as next year. The challenges faced from producing and analyzing this magnitude of events in parallel have raised issues with regard to the distribution of calibrations and geometry data, via databases, to STAR's growing global collaboration. Rapid distribution, availability, ensured synchronization and load balancing have become paramount considerations. Both conventional technology and novel approaches are used in parallel to realize these goals. This paper discusses how STAR uses load balancing to optimize database usage. It discusses distribution methods via MySQL master slave replication; the synchronization issues that arise from this type of distribution and solutions, mostly homegrown, put forth to overcome these issues. A novel approach toward load balancing between slave nodes that assists in maintaining a high availability rate for a veracious community is discussed in detail. This load balancing addresses both, pools of nodes internal to a given location, as well as balancing the load for remote users between different available locations. Challenges, trade-offs, rationale for decisions and paths forward will be discussed in all cases, presenting a solid production environment with a vision for scalable growth

  5. Automated replication of cone beam CT-guided treatments in the Pinnacle(3) treatment planning system for adaptive radiotherapy.

    Science.gov (United States)

    Hargrave, Catriona; Mason, Nicole; Guidi, Robyn; Miller, Julie-Anne; Becker, Jillian; Moores, Matthew; Mengersen, Kerrie; Poulsen, Michael; Harden, Fiona

    2016-03-01

    Time-consuming manual methods have been required to register cone-beam computed tomography (CBCT) images with plans in the Pinnacle(3) treatment planning system in order to replicate delivered treatments for adaptive radiotherapy. These methods rely on fiducial marker (FM) placement during CBCT acquisition or the image mid-point to localise the image isocentre. A quality assurance study was conducted to validate an automated CBCT-plan registration method utilising the Digital Imaging and Communications in Medicine (DICOM) Structure Set (RS) and Spatial Registration (RE) files created during online image-guided radiotherapy (IGRT). CBCTs of a phantom were acquired with FMs and predetermined setup errors using various online IGRT workflows. The CBCTs, DICOM RS and RE files were imported into Pinnacle(3) plans of the phantom and the resulting automated CBCT-plan registrations were compared to existing manual methods. A clinical protocol for the automated method was subsequently developed and tested retrospectively using CBCTs and plans for six bladder patients. The automated CBCT-plan registration method was successfully applied to thirty-four phantom CBCT images acquired with an online 0 mm action level workflow. Ten CBCTs acquired with other IGRT workflows required manual workarounds. This was addressed during the development and testing of the clinical protocol using twenty-eight patient CBCTs. The automated CBCT-plan registrations were instantaneous, replicating delivered treatments in Pinnacle(3) with errors of ±0.5 mm. These errors were comparable to mid-point-dependant manual registrations but superior to FM-dependant manual registrations. The automated CBCT-plan registration method quickly and reliably replicates delivered treatments in Pinnacle(3) for adaptive radiotherapy.

  6. Generic device controller for accelerator control systems

    International Nuclear Information System (INIS)

    Mariotti, R.; Buxton, W.; Frankel, R.; Hoff, L.

    1987-01-01

    Distributed intelligence for accelerator control systems has become possible as a result of advances in microprocessor technology. A system based on distributed intelligence is inherently versatile, readily expandable, and reduces both information flow across the system and software complexity in each unit

  7. Coordination control of distributed systems

    CERN Document Server

    Villa, Tiziano

    2015-01-01

    This book describes how control of distributed systems can be advanced by an integration of control, communication, and computation. The global control objectives are met by judicious combinations of local and nonlocal observations taking advantage of various forms of communication exchanges between distributed controllers. Control architectures are considered according to  increasing degrees of cooperation of local controllers:  fully distributed or decentralized controlcontrol with communication between controllers,  coordination control, and multilevel control.  The book covers also topics bridging computer science, communication, and control, like communication for control of networks, average consensus for distributed systems, and modeling and verification of discrete and of hybrid systems. Examples and case studies are introduced in the first part of the text and developed throughout the book. They include: control of underwater vehicles, automated-guided vehicles on a container terminal, contro...

  8. Division 1137 property control system

    Energy Technology Data Exchange (ETDEWEB)

    Pastor, D.J.

    1982-01-01

    An automated data processing property control system was developed by Mobile and Remote Range Division 1137. This report describes the operation of the system and examines ways of using it in operational planning and control.

  9. Generic device controller for accelerator control systems

    International Nuclear Information System (INIS)

    Mariotti, R.; Buxton, W.; Frankel, R.; Hoff, L.

    1987-01-01

    A new distributed intelligence control system has become operational at the AGS for transport, injection, and acceleration of heavy ions. A brief description of the functionality of the physical devices making up the system is given. An attempt has been made to integrate the devices for accelerator specific interfacing into a standard microprocessor system, namely, the Universal Device Controller (UDC). The main goals for such a generic device controller are to provide: local computing power; flexibility to configure; and real time event handling. The UDC assemblies and software are described

  10. On Restructurable Control System Theory

    Science.gov (United States)

    Athans, M.

    1983-01-01

    The state of stochastic system and control theory as it impacts restructurable control issues is addressed. The multivariable characteristics of the control problem are addressed. The failure detection/identification problem is discussed as a multi-hypothesis testing problem. Control strategy reconfiguration, static multivariable controls, static failure hypothesis testing, dynamic multivariable controls, fault-tolerant control theory, dynamic hypothesis testing, generalized likelihood ratio (GLR) methods, and adaptive control are discussed.

  11. Hybrid spacecraft attitude control system

    OpenAIRE

    Renuganth Varatharajoo; Ramly Ajir; Tamizi Ahmad

    2016-01-01

    The hybrid subsystem design could be an attractive approach for futurespacecraft to cope with their demands. The idea of combining theconventional Attitude Control System and the Electrical Power System ispresented in this article. The Combined Energy and Attitude ControlSystem (CEACS) consisting of a double counter rotating flywheel assemblyis investigated for small satellites in this article. Another hybrid systemincorporating the conventional Attitude Control System into the ThermalControl...

  12. Characterization of gradient control systems

    NARCIS (Netherlands)

    Cortés, Jorge; van der Schaft, Arjan; Crouch, Peter E.

    2005-01-01

    Given a general nonlinear affine control system with outputs and a torsion-free affine connection defined on its state space, we investigate the gradient realization problem: we give necessary and sufficient conditions under which the control system can be written as a gradient control system

  13. Characterization of Gradient Control Systems

    NARCIS (Netherlands)

    Cortés, Jorge; Schaft, Arjan van der; Crouch, Peter E.

    2005-01-01

    Given a general nonlinear affine control system with outputs and a torsion-free affine connection defined on its state space, we investigate the gradient realization problem: we give necessary and sufficient conditions under which the control system can be written as a gradient control system

  14. Using Wireless Response Systems to Replicate Behavioral Research Findings in the Classroom

    Science.gov (United States)

    Cleary, Anne M.

    2008-01-01

    College instructors are increasingly relying on wireless clicker systems as instructional tools in the classroom. Instructors commonly use clicker systems for such classroom activities as taking attendance, giving quizzes, and taking opinion polls. However, these systems are uniquely well suited for the teaching of psychology and other courses…

  15. Networked control of microgrid system of systems

    Science.gov (United States)

    Mahmoud, Magdi S.; Rahman, Mohamed Saif Ur; AL-Sunni, Fouad M.

    2016-08-01

    The microgrid has made its mark in distributed generation and has attracted widespread research. However, microgrid is a complex system which needs to be viewed from an intelligent system of systems perspective. In this paper, a network control system of systems is designed for the islanded microgrid system consisting of three distributed generation units as three subsystems supplying a load. The controller stabilises the microgrid system in the presence of communication infractions such as packet dropouts and delays. Simulation results are included to elucidate the effectiveness of the proposed control strategy.

  16. Covariance upperbound controllers for networked control systems

    International Nuclear Information System (INIS)

    Ko, Sang Ho

    2012-01-01

    This paper deals with designing covariance upperbound controllers for a linear system that can be used in a networked control environment in which control laws are calculated in a remote controller and transmitted through a shared communication link to the plant. In order to compensate for possible packet losses during the transmission, two different techniques are often employed: the zero-input and the hold-input strategy. These use zero input and the latest control input, respectively, when a packet is lost. For each strategy, we synthesize a class of output covariance upperbound controllers for a given covariance upperbound and a packet loss probability. Existence conditions of the covariance upperbound controller are also provided for each strategy. Through numerical examples, performance of the two strategies is compared in terms of feasibility of implementing the controllers

  17. Entry-Control Systems Handbook

    International Nuclear Information System (INIS)

    1978-09-01

    The function of an entry-control system in a total Physical Protection System is to allow the movement of authorized personnel and material through normal access routes, yet detect and delay unauthorized movement of personnel and material from uncontrolled areas. The ten chapters of this handbook cover: introduction, credentials, personnel identity verification systems, special nuclear materials monitors, metal detectors, explosives sensors, package search systems, criteria for selection of entry-control equipment, machine-aided manual entry-control systems, and automated entry-control systems. A system example and its cost are included as an appendix

  18. VOC emissions control systems

    International Nuclear Information System (INIS)

    Spessard, J.E.

    1993-01-01

    The air pollution control equipment marketplace offers many competing technologies for controlling emissions of volatile organic compounds (VOC) in air. If any technology was economically and technically superior under all conditions, it would be the only one on the market. In fact, each technology used to control VOCs is superior under some set of conditions. The reasons for choosing one control technology over another are situation-specific. Some general guidelines to VOC control technologies and the situations where each may be appropriate are presented in this article. The control technologies and applications are summarized in a table

  19. Docosahexaenoic acid for reading, working memory and behavior in UK children aged 7-9: A randomized controlled trial for replication (the DOLAB II study).

    Science.gov (United States)

    Montgomery, Paul; Spreckelsen, Thees F; Burton, Alice; Burton, Jennifer R; Richardson, Alexandra J

    2018-01-01

    Omega-3 fatty acids are central to brain-development of children. Evidence from clinical trials and systematic reviews demonstrates the potential of long-chain Omega-3 supplementation for learning and behavior. However, findings are inconclusive and in need of robust replication studies since such work is lacking. Replication of the 2012 DOLAB 1 study findings that a dietary supplementation with the long-chain omega-3 docosahexaenoic acid (DHA) had beneficial effects on the reading, working memory, and behavior of healthy schoolchildren. Parallel group, fixed-dose, randomized (minimization, 30% random element), double-blind, placebo-controlled trial (RCT). Mainstream primary schools (n = 84) from five counties in the UK in 2012-2015. Healthy children aged 7-9 underperforming in reading (reading, working memory, and behavior, parent-rated and as secondary outcome teacher-rated. 376 children were randomized. Reading, working memory, and behavior change scores showed no consistent differences between intervention and placebo group. Some behavioral subscales showed minor group differences. This RCT did not replicate results of the earlier DOLAB 1 study on the effectiveness of nutritional supplementation with DHA for learning and behavior. Possible reasons are discussed, particularly regarding the replication of complex interventions. www.controlled-trials.com (ISRCTN48803273) and protocols.io (https://dx.doi.org/10.17504/protocols.io.k8kczuw).

  20. Systems and Control Engineering

    Indian Academy of Sciences (India)

    activities directed towards the students and the general public. Designed .... attention has been directed towards the use of control and automation to mitigate the effects of those ... The history of automatic control can be divided into four main.

  1. System for controlling apnea

    Science.gov (United States)

    Holzrichter, John F

    2015-05-05

    An implanted stimulation device or air control device are activated by an external radar-like sensor for controlling apnea. The radar-like sensor senses the closure of the air flow cavity, and associated control circuitry signals (1) a stimulator to cause muscles to open the air passage way that is closing or closed or (2) an air control device to open the air passage way that is closing or closed.

  2. SRS control system upgrade requirements

    International Nuclear Information System (INIS)

    Hill, L.F.

    1998-01-01

    This document defines requirements for an upgrade of the Sodium Removal System (SRS) control system. The upgrade is being performed to solve a number of maintainability and operability issues. The upgraded system will provide the same functions, controls and interlocks as the present system, and in addition provide enhanced functionality in areas discussed in this document

  3. Expert systems in process control systems

    International Nuclear Information System (INIS)

    Wittig, T.

    1987-01-01

    To illustrate where the fundamental difference between expert systems in classical diagnosis and in industrial control lie, the work of process control instrumentation is used as an example for the job of expert systems. Starting from the general process of problem-solving, two classes of expert systems can be defined accordingly. (orig.) [de

  4. Modernization of control system using the digital control system

    International Nuclear Information System (INIS)

    Carrasco, J. A.; Fernandez, L.; Jimenez, A.

    2002-01-01

    Nowadays, all plant automation tendencies are based on the use of Digital Control System. In big industrial plants the control systems employed are Distributed Control Systems (DCS). The addition of these systems in nuclear power plants,implies an important adaptation process, because most of them were installed using analog control systems. This paper presents the objectives and the first results obtained, in a modernization project, focused in obtaining an engineering platform for making test and analysis of changes prior to their implementation in a nuclear plant. Modernization, Upgrade, DCS, Automation, Simulation, Training. (Author)

  5. Distributed systems status and control

    Science.gov (United States)

    Kreidler, David; Vickers, David

    1990-01-01

    Concepts are investigated for an automated status and control system for a distributed processing environment. System characteristics, data requirements for health assessment, data acquisition methods, system diagnosis methods and control methods were investigated in an attempt to determine the high-level requirements for a system which can be used to assess the health of a distributed processing system and implement control procedures to maintain an accepted level of health for the system. A potential concept for automated status and control includes the use of expert system techniques to assess the health of the system, detect and diagnose faults, and initiate or recommend actions to correct the faults. Therefore, this research included the investigation of methods by which expert systems were developed for real-time environments and distributed systems. The focus is on the features required by real-time expert systems and the tools available to develop real-time expert systems.

  6. Novelty vs. Replicability : Virtues and Vices in the Reward System of Science

    NARCIS (Netherlands)

    Romero Toro, Felipe

    2017-01-01

    The reward system of science is the priority rule (Merton, 1957). The first scientist making a new discovery is rewarded with prestige while second runners get little or nothing. Strevens (2003, 2011), following Kitcher (1990), defends this reward system arguing that it incentivizes an efficient

  7. Replicating the Ice-Volume Signal of the Early Pleistocene with a Complex Earth System Model

    Science.gov (United States)

    Tabor, C. R.; Poulsen, C. J.; Pollard, D.

    2013-12-01

    Milankovitch theory proposes high-latitude summer insolation intensity paces the ice ages by controlling perennial snow cover amounts (Milankovitch, 1941). According to theory, the ~21 kyr cycle of precession should dominate the ice-volume records since it has the greatest influence on high-latitude summer insolation. Modeling experiments frequently support Milankovitch theory by attributing the majority of Northern Hemisphere high-latitude summer snowmelt to changes in the cycle of precession (e.g. Jackson and Broccoli, 2003). However, ice-volume proxy records, especially those of the Early Pleistocene (2.6-0.8 Ma), display variability with a period of ~41 kyr (Raymo and Lisiecki, 2005), indicative of insolation forcing from obliquity, which has a much smaller influence on summer insolation intensity than precession. Several hypotheses attempt to explain the discrepancies between Milkankovitch theory and the proxy records by invoking phenomena such as insolation gradients (Raymo and Nisancioglu, 2003), hemispheric offset (Raymo et al., 2006; Lee and Poulsen, 2009), and integrated summer energy (Huybers, 2006); however, all of these hypotheses contain caveats (Ruddiman, 2006) and have yet to be supported by modeling studies that use a complex GCM. To explore potential solutions to this '41 kyr problem,' we use an Earth system model composed of the GENESIS GCM and Land Surface model, the BIOME4 vegetation model, and the Pennsylvania State ice-sheet model. Using an asynchronous coupling technique, we run four idealized transient combinations of obliquity and precession, representing the orbital extremes of the Pleistocene (Berger and Loutre, 1991). Each experiment is run through several complete orbital cycles with a dynamic ice domain spanning North America and Greenland, and fixed preindustrial greenhouse-gas concentrations. For all orbital configurations, model results produce greater ice-volume spectral power at the frequency of obliquity despite significantly

  8. Upgrading the BEPC control system

    International Nuclear Information System (INIS)

    Yang Liping; Wang Lizheng; Liu Shiyao

    1992-01-01

    The BEPC control system has been put into operation and operated normally since the end of 1987. Three years's experience shows this system can satisfy basically the operation requirements, also exhibits some disadvantages araised from the original centralized system architecture based on the VAX-VCC-CAMAC, such as slow response, bottle neck of VCC, less CPU power for control etc.. This paper describes the method and procedure for upgrading the BEPC control system which will be based on DEC net and DEC-WS, and thus intend to upgrade the control system architecture from the centralized to the distributed and improve the integral system performance. (author)

  9. SPring-8 beamline control system.

    Science.gov (United States)

    Ohata, T; Konishi, H; Kimura, H; Furukawa, Y; Tamasaku, K; Nakatani, T; Tanabe, T; Matsumoto, N; Ishii, M; Ishikawa, T

    1998-05-01

    The SPring-8 beamline control system is now taking part in the control of the insertion device (ID), front end, beam transportation channel and all interlock systems of the beamline: it will supply a highly standardized environment of apparatus control for collaborative researchers. In particular, ID operation is very important in a third-generation synchrotron light source facility. It is also very important to consider the security system because the ID is part of the storage ring and is therefore governed by the synchrotron ring control system. The progress of computer networking systems and the technology of security control require the development of a highly flexible control system. An interlock system that is independent of the control system has increased the reliability. For the beamline control system the so-called standard model concept has been adopted. VME-bus (VME) is used as the front-end control system and a UNIX workstation as the operator console. CPU boards of the VME-bus are RISC processor-based board computers operated by a LynxOS-based HP-RT real-time operating system. The workstation and the VME are linked to each other by a network, and form the distributed system. The HP 9000/700 series with HP-UX and the HP 9000/743rt series with HP-RT are used. All the controllable apparatus may be operated from any workstation.

  10. Hybrid spacecraft attitude control system

    Directory of Open Access Journals (Sweden)

    Renuganth Varatharajoo

    2016-02-01

    Full Text Available The hybrid subsystem design could be an attractive approach for futurespacecraft to cope with their demands. The idea of combining theconventional Attitude Control System and the Electrical Power System ispresented in this article. The Combined Energy and Attitude ControlSystem (CEACS consisting of a double counter rotating flywheel assemblyis investigated for small satellites in this article. Another hybrid systemincorporating the conventional Attitude Control System into the ThermalControl System forming the Combined Attitude and Thermal ControlSystem (CATCS consisting of a "fluid wheel" and permanent magnets isalso investigated for small satellites herein. The governing equationsdescribing both these novel hybrid subsystems are presented and theironboard architectures are numerically tested. Both the investigated novelhybrid spacecraft subsystems comply with the reference missionrequirements.The hybrid subsystem design could be an attractive approach for futurespacecraft to cope with their demands. The idea of combining theconventional Attitude Control System and the Electrical Power System ispresented in this article. The Combined Energy and Attitude ControlSystem (CEACS consisting of a double counter rotating flywheel assemblyis investigated for small satellites in this article. Another hybrid systemincorporating the conventional Attitude Control System into the ThermalControl System forming the Combined Attitude and Thermal ControlSystem (CATCS consisting of a "fluid wheel" and permanent magnets isalso investigated for small satellites herein. The governing equationsdescribing both these novel hybrid subsystems are presented and theironboard architectures are numerically tested. Both the investigated novelhybrid spacecraft subsystems comply with the reference missionrequirements.

  11. Diagnostic, reliablility and control systems

    CERN Document Server

    Leondes

    2014-01-01

    1. Explicit-Model-Based Fault Detection Method in Industrial Plants 2. Soft Sensor: An Effective Approach to Improve Control 3. Techniques in Soft Computing and Their Utilization in Mechatronic Products 4. Techniques in the Control of Interconnected Plants 5. A Mechatronic Systems Approach to Controlling Robotic Systems with Actuator Dynamics 6. Process and Control Design for Fast Coordinate Measuring Machines 7. Techniques in the Stability of Mechatronic Systems with Sensor or Actuator Failure.

  12. Integration Platform As Central Service Of Data Replication In Distributed Medical System

    Directory of Open Access Journals (Sweden)

    Wiesław Wajs

    2007-01-01

    Full Text Available The paper presents the application of Java Integration Platform (JIP to data replicationin the distributed medical system. After an introductory part on the medical system’s architecture,the focus shifts to a comparison of different approaches that exist with regard totransferring data between the system’s components. A description is given of the historicaldata processing and of the whole area of the JIP application to the medical system.

  13. Metabolic, Replication and Genomic Category of Systems in Biology, Bioinformatics and Medicine

    OpenAIRE

    I. C. Baianu

    2012-01-01

    Metabolic-repair models, or (M,R)-systems were introduced in Relational Biology by Robert Rosen. Subsequently, Rosen represented such (M,R)-systems (or simply MRs)in terms of categories of sets, deliberately selected without any structure other than the discrete topology of sets. Theoreticians of life’s origins postulated that Life on Earth has begun with the simplest possible organism, called the primordial. Mathematicians interested in biology attempted to answer this important quest...

  14. D0 Cryo System Control System Autodialer

    Energy Technology Data Exchange (ETDEWEB)

    Urbin, J.; /Fermilab

    1990-04-17

    The DO cryogenic system is controlled by a TI565-PLC based control system. This allows the system to be unmanned when in steady state operation. System experts will need to be contacted when system parameters exceed normal operating points and reach alarm setpoints. The labwide FIRUS system provides one alarm monitor and communication link. An autodialer provides a second and more flexible alarm monitor and communication link. The autodialer monitors contact points in the control system and after receiving indication of an alarm accesses a list of experts which it calls until it receives an acknowledgement. There are several manufacturers and distributors of autodialer systems. This EN explains the search process the DO cryo group used to fmd an autodialer system that fit the cryo system's needs and includes information and specs for the unit we chose.

  15. Autorotation flight control system

    Science.gov (United States)

    Bachelder, Edward N. (Inventor); Lee, Dong-Chan (Inventor); Aponso, Bimal L. (Inventor)

    2011-01-01

    The present invention provides computer implemented methodology that permits the safe landing and recovery of rotorcraft following engine failure. With this invention successful autorotations may be performed from well within the unsafe operating area of the height-velocity profile of a helicopter by employing the fast and robust real-time trajectory optimization algorithm that commands control motion through an intuitive pilot display, or directly in the case of autonomous rotorcraft. The algorithm generates optimal trajectories and control commands via the direct-collocation optimization method, solved using a nonlinear programming problem solver. The control inputs computed are collective pitch and aircraft pitch, which are easily tracked and manipulated by the pilot or converted to control actuator commands for automated operation during autorotation in the case of an autonomous rotorcraft. The formulation of the optimal control problem has been carefully tailored so the solutions resemble those of an expert pilot, accounting for the performance limitations of the rotorcraft and safety concerns.

  16. Characterizing the Final Steps of Chromosomal Replication at the Single-molecule Level in the Model System Escherichia coli

    KAUST Repository

    Elshenawy, Mohamed M.

    2015-12-01

    In the circular Escherichia coli chromosome, two replisomes are assembled at the unique origin of replication and drive DNA synthesis in opposite directions until they meet in the terminus region across from the origin. Despite the difference in rates of the two replisomes, their arrival at the terminus is synchronized through a highly specialized system consisting of the terminator protein (Tus) bound to the termination sites (Ter). This synchronicity is mediated by the polarity of the Tus−Ter complex that stops replisomes from one direction (non-permissive face) but not the other (permissive face). Two oppositely oriented clusters of five Tus–Ters that each block one of the two replisomes create a “replication fork trap” for the first arriving replisome while waiting for the late arriving one. Despite extensive biochemical and structural studies, the molecular mechanism behind Tus−Ter polar arrest activity remained controversial. Moreover, none of the previous work provided answers for the long-standing discrepancy between the ability of Tus−Ter to permanently stop replisomes in vitro and its low efficiency in vivo. Here, I spearheaded a collaborative project that combined single-molecule DNA replication assays, X-ray crystallography and binding studies to provide a true molecular-level understanding of the underlying mechanism of Tus−Ter polar arrest activity. We showed that efficiency of Tus−Ter is determined by a head-to-head kinetic competition between rate of strand separation by the replisome and rate of rearrangement of Tus−Ter interactions during the melting of the first 6 base pairs of Ter. This rearrangement maintains Tus’s strong grip on the DNA and stops the advancing replisome from breaking into Tus−Ter central interactions, but only transiently. We further showed how this kinetic competition functions within the context of two mechanisms to impose permanent fork stoppage. The rate-dependent fork arrest activity of Tus

  17. Framework for control system development

    International Nuclear Information System (INIS)

    Cork, C.; Nishimura, Hiroshi

    1992-01-01

    Control systems being developed for the present generation of accelerators will need to adapt to changing machine and operating state conditions. Such systems must also be capable of evolving over the life of the accelerator operation. In this paper we present a framework for the development of adaptive control systems

  18. Framework for control system development

    International Nuclear Information System (INIS)

    Cork, C.; Nishimura, Hiroshi.

    1991-11-01

    Control systems being developed for the present generation of accelerators will need to adapt to changing machine and operating state conditions. Such systems must also be capable of evolving over the life of the accelerator operation. In this paper we present a framework for the development of adaptive control systems

  19. The GSI control system

    International Nuclear Information System (INIS)

    Krause, U.; Schaa, V.; Steiner, R.

    1992-01-01

    The GSI accelerator facility consists of an old linac and two modern machines, a synchrotron and a storage ring. It is operated from one control room. Only three operators at a time have to keep it running with only little assistance from machine specialists in daytime. So the control tools must provide a high degree of abstraction and modeling to relieve the operators from details on the device level. The program structures to achieve this are described in this paper. A coarse overview of the control architecture is given. (author)

  20. Minicomputer controlled test system for process control and monitoring systems

    International Nuclear Information System (INIS)

    Worster, L.D.

    A minicomputer controlled test system for testing process control and monitoring systems is described. This system, in service for over one year, has demonstrated that computerized control of such testing has a real potential for expanding the scope of the testing, improving accuracy of testing, and significantly reducing the time required to do the testing. The test system is built around a 16-bit minicomputer with 12K of memory. The system programming language is BASIC with the addition of assembly level routines for communication with the peripheral devices. The peripheral devices include a 100 channel scanner, analog-to-digital converter, visual display, and strip printer. (auth)

  1. A trade-off in replication in mosquito versus mammalian systems conferred by a point mutation in the NS4B protein of dengue virus type 4

    International Nuclear Information System (INIS)

    Hanley, Kathryn A.; Manlucu, Luella R.; Gilmore, Lara E.; Blaney, Joseph E.; Hanson, Christopher T.; Murphy, Brian R.; Whitehead, Stephen S.

    2003-01-01

    An acceptable live-attenuated dengue virus vaccine candidate should have low potential for transmission by mosquitoes. We have identified and characterized a mutation in dengue virus type 4 (DEN4) that decreases the ability of the virus to infect mosquitoes. A panel of 1248 mutagenized virus clones generated previously by chemical mutagenesis was screened for decreased replication in mosquito C6/36 cells but efficient replication in simian Vero cells. One virus met these criteria and contained a single coding mutation: a C-to-U mutation at nucleotide 7129 resulting in a Pro-to-Leu change in amino acid 101 of the nonstructural 4B gene (NS4B P101L). This mutation results in decreased replication in C6/36 cells relative to wild-type DEN4, decreased infectivity for mosquitoes, enhanced replication in Vero and human HuH-7 cells, and enhanced replication in SCID mice implanted with HuH-7 cells (SCID-HuH-7 mice). A recombinant DEN4 virus (rDEN4) bearing this mutation exhibited the same set of phenotypes. Addition of the NS4B P101L mutation to rDEN4 bearing a 30 nucleotide deletion (Δ30) decreased the ability of the double-mutant virus to infect mosquitoes but increased its ability to replicate in SCID-HuH-7 mice. Although the NS4B P101L mutation decreases infectivity of DEN4 for mosquitoes, its ability to enhance replication in SCID-HuH-7 mice suggests that it might not be advantageous to include this specific mutation in an rDEN4 vaccine. The opposing effects of the NS4B P101L mutation in mosquito and vertebrate systems suggest that the NS4B protein is involved in maintaining the balance between efficient replication in the mosquito vector and the human host

  2. Systems and Control Engineering

    Indian Academy of Sciences (India)

    design of civil engineering structures has been noted. Protecting ci vil ... R despite disturbing forces such as wind gusts, changes in ambient temperature, etc .. Brief History of ... frequency regulation, boiler control for steam generation, electric.

  3. Controlling Uncertain Dynamical Systems

    Indian Academy of Sciences (India)

    Author Affiliations. N Ananthkrishnan1 Rashi Bansal2. Head, CAE Analysis & Design Zeus Numerix Pvt Ltd. M-03, SINE, IIT Bombay Powai Mumbai 400076, India. MTech (Aerospace Engineering) with specialization in Dynamics & Control from IIT Bombay.

  4. Delays and networked control systems

    CERN Document Server

    Hetel, Laurentiu; Daafouz, Jamal; Johansson, Karl

    2016-01-01

    This edited monograph includes state-of-the-art contributions on continuous time dynamical networks with delays. The book is divided into four parts. The first part presents tools and methods for the analysis of time-delay systems with a particular attention on control problems of large scale or infinite-dimensional systems with delays. The second part of the book is dedicated to the use of time-delay models for the analysis and design of Networked Control Systems. The third part of the book focuses on the analysis and design of systems with asynchronous sampling intervals which occur in Networked Control Systems. The last part of the book exposes several contributions dealing with the design of cooperative control and observation laws for networked control systems. The target audience primarily comprises researchers and experts in the field of control theory, but the book may also be beneficial for graduate students. .

  5. Control integral systems; Sistemas integrales de control

    Energy Technology Data Exchange (ETDEWEB)

    Burgos, Estrella [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1999-12-31

    Almost two third of the electric power generation in Mexico are obtained from hydrocarbons, for that reasons Comision Federal de Electricidad (CFE) dedicated special commitment in modernizing the operation of fossil fuel central stations. In attaining this objective the control systems play a fundamental roll, from them depend a good share of the reliability and the efficiency of the electric power generation process, as well as the extension of the equipment useful life. Since 1984 the Instituto de Investigaciones Electricas (IIE) has been working, upon the request of CFE, on the development of digital control systems. To date it has designed and implemented a logic control system for gas burners, which controls 32 burners of the Unit 4 boiler of the Generation Central of Valle de Mexico and two systems for distributed control for two combined cycle central stations, which are: Dos Bocas, Veracruz Combined cycle central, and Gomez Palacio, Durango combined cycle central. With these two developments the IIE enters the World tendency of implementing distributed control systems for the fossil fuel power central update [Espanol] Casi las dos terceras partes de la generacion electrica en Mexico se obtienen a partir de hidrocarburos, es por eso que la Comision Federal de Electricidad (CFE) puso especial empeno en modernizar la operacion de las centrales termoelectricas de combustibles fosiles. En el logro de este objetivo los sistemas de control desempenan un papel fundamental, de ellos depende una buena parte la confiabilidad y la eficiencia en el proceso de generacion de energia electrica, asi como la prolongacion de la vida util de los equipos. Desde 1984 el Instituto de Investigaciones Electricas (IIE) ha trabajado, a solicitud de la CFE, en el desarrollo de sistemas digitales de control. A la fecha se han disenado e implantado un sistema de control logico de quemadores de gas, el cual controla 32 quemadores de la caldera de la unidad 4 de la central de generacion

  6. Control integral systems; Sistemas integrales de control

    Energy Technology Data Exchange (ETDEWEB)

    Burgos, Estrella [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1998-12-31

    Almost two third of the electric power generation in Mexico are obtained from hydrocarbons, for that reasons Comision Federal de Electricidad (CFE) dedicated special commitment in modernizing the operation of fossil fuel central stations. In attaining this objective the control systems play a fundamental roll, from them depend a good share of the reliability and the efficiency of the electric power generation process, as well as the extension of the equipment useful life. Since 1984 the Instituto de Investigaciones Electricas (IIE) has been working, upon the request of CFE, on the development of digital control systems. To date it has designed and implemented a logic control system for gas burners, which controls 32 burners of the Unit 4 boiler of the Generation Central of Valle de Mexico and two systems for distributed control for two combined cycle central stations, which are: Dos Bocas, Veracruz Combined cycle central, and Gomez Palacio, Durango combined cycle central. With these two developments the IIE enters the World tendency of implementing distributed control systems for the fossil fuel power central update [Espanol] Casi las dos terceras partes de la generacion electrica en Mexico se obtienen a partir de hidrocarburos, es por eso que la Comision Federal de Electricidad (CFE) puso especial empeno en modernizar la operacion de las centrales termoelectricas de combustibles fosiles. En el logro de este objetivo los sistemas de control desempenan un papel fundamental, de ellos depende una buena parte la confiabilidad y la eficiencia en el proceso de generacion de energia electrica, asi como la prolongacion de la vida util de los equipos. Desde 1984 el Instituto de Investigaciones Electricas (IIE) ha trabajado, a solicitud de la CFE, en el desarrollo de sistemas digitales de control. A la fecha se han disenado e implantado un sistema de control logico de quemadores de gas, el cual controla 32 quemadores de la caldera de la unidad 4 de la central de generacion

  7. Asynchronous control for networked systems

    CERN Document Server

    Rubio, Francisco; Bencomo, Sebastián

    2015-01-01

    This book sheds light on networked control systems; it describes different techniques for asynchronous control, moving away from the periodic actions of classical control, replacing them with state-based decisions and reducing the frequency with which communication between subsystems is required. The text focuses specially on event-based control. Split into two parts, Asynchronous Control for Networked Systems begins by addressing the problems of single-loop networked control systems, laying out various solutions which include two alternative model-based control schemes (anticipatory and predictive) and the use of H2/H∞ robust control to deal with network delays and packet losses. Results on self-triggering and send-on-delta sampling are presented to reduce the need for feedback in the loop. In Part II, the authors present solutions for distributed estimation and control. They deal first with reliable networks and then extend their results to scenarios in which delays and packet losses may occur. The novel ...

  8. Standardization of detector control systems

    International Nuclear Information System (INIS)

    Fukunaga, Chikara

    2000-01-01

    Current and future detectors for high-energy and/or nuclear physics experiments require highly intelligent detector control systems. In order to reduce resources, the construction of a standardized template for the control systems based on the commercially available superviser control and data acquisition (SCADA) system has been proposed. The possibility of constructing this template is discussed and several key issues for evaluation of SCADA as the basis for such a template are presented. (author)

  9. Lighting Control System (ILCS)

    African Journals Online (AJOL)

    2017-08-08

    Aug 8, 2017 ... function blocks CNC machining protocol. Advanced Materials Research, 2014, 845:779-785. [2] Miki M, Nagano M, Yoshimi M, Yonemoto H, Yoshida K. Intelligent lighting system with an additional energy-saving mechanism. In IEEE International Conference on Systems,. Man, and Cybernetics, 2012, pp.

  10. A theory for the origin of a self-replicating chemical system. I - Natural selection of the autogen from short, random oligomers

    Science.gov (United States)

    White, D. H.

    1980-01-01

    A general theory is presented for the origin of a self-replicating chemical system, termed an autogen, which is capable of both crude replication and translation (protein synthesis). The theory requires the availability of free energy and monomers to the system, a significant background low-yield synthesis of kinetically stable oligopeptides and oligonucleotides, the localization of the oligomers, crude oligonucleotide selectivity of amino acids during oligopeptide synthesis, crude oligonucleotide replication, and two short peptide families which catalyze replication and translation, to produce a localized group of at least one copy each of two protogenes and two protoenzymes. The model posits a process of random oligomerization, followed by the random nucleation of functional components and the rapid autocatalytic growth of the functioning autogen to macroscopic amounts, to account for the origin of the first self-replicating system. Such a process contains steps of such high probability and short time periods that it is suggested that the emergence of an autogen in a laboratory experiment of reasonable time scale may be possible.

  11. AUTOMATIC FREQUENCY CONTROL SYSTEM

    Science.gov (United States)

    Hansen, C.F.; Salisbury, J.D.

    1961-01-10

    A control is described for automatically matching the frequency of a resonant cavity to that of a driving oscillator. The driving oscillator is disconnected from the cavity and a secondary oscillator is actuated in which the cavity is the frequency determining element. A low frequency is mixed with the output of the driving oscillator and the resultant lower and upper sidebands are separately derived. The frequencies of the sidebands are compared with the secondary oscillator frequency. deriving a servo control signal to adjust a tuning element in the cavity and matching the cavity frequency to that of the driving oscillator. The driving oscillator may then be connected to the cavity.

  12. Reactor power control system

    International Nuclear Information System (INIS)

    Tomisawa, Teruaki.

    1981-01-01

    Purpose: To restore reactor-power condition in a minimum time after a termination of turbine bypass by reducing the throttling of the reactor power at the time of load-failure as low as possible. Constitution: The transient change of the internal pressure of condenser is continuously monitored. When a turbine is bypassed, a speed-control-command signal for a coolant recirculating pump is generated according as the internal pressure of the condenser. When the signal relating to the internal pressure of the condenser indicates insufficient power, a reactor-control-rod-drive signal is generated. (J.P.N.)

  13. Ground Control System Description Document

    International Nuclear Information System (INIS)

    Eric Loros

    2001-01-01

    The Ground Control System contributes to the safe construction and operation of the subsurface facility, including accesses and waste emplacement drifts, by maintaining the configuration and stability of the openings during construction, development, emplacement, and caretaker modes for the duration of preclosure repository life. The Ground Control System consists of ground support structures installed within the subsurface excavated openings, any reinforcement made to the rock surrounding the opening, and inverts if designed as an integral part of the system. The Ground Control System maintains stability for the range of geologic conditions expected at the repository and for all expected loading conditions, including in situ rock, construction, operation, thermal, and seismic loads. The system maintains the size and geometry of operating envelopes for all openings, including alcoves, accesses, and emplacement drifts. The system provides for the installation and operation of sensors and equipment for any required inspection and monitoring. In addition, the Ground Control System provides protection against rockfall for all subsurface personnel, equipment, and the engineered barrier system, including the waste package during the preclosure period. The Ground Control System uses materials that are sufficiently maintainable and that retain the necessary engineering properties for the anticipated conditions of the preclosure service life. These materials are also compatible with postclosure waste isolation performance requirements of the repository. The Ground Control System interfaces with the Subsurface Facility System for operating envelopes, drift orientation, and excavated opening dimensions, Emplacement Drift System for material compatibility, Monitored Geologic Repository Operations Monitoring and Control System for ground control instrument readings, Waste Emplacement/Retrieval System to support waste emplacement operations, and the Subsurface Excavation System

  14. Control Allocation for Overactuated Systems

    National Research Council Canada - National Science Library

    Oppenheimer, Michael W; Doman, David B

    2006-01-01

    Much emphasis has been placed on overactuated systems for air vehicles. Overactuating an air vehicle provides a certain amount of redundancy for the flight control system, thus potentially allowing for recovery from off-nominal conditions...

  15. The APS control system network

    International Nuclear Information System (INIS)

    Sidorowicz, K.V.; McDowell, W.P.

    1995-01-01

    The APS accelerator control system is a distributed system consisting of operator interfaces, a network, and computer-controlled interfaces to hardware. This implementation of a control system has come to be called the open-quotes Standard Model.close quotes The operator interface is a UNDC-based workstation with an X-windows graphical user interface. The workstation may be located at any point on the facility network and maintain full functionality. The function of the network is to provide a generalized communication path between the host computers, operator workstations, input/output crates, and other hardware that comprise the control system. The crate or input/output controller (IOC) provides direct control and input/output interfaces for each accelerator subsystem. The network is an integral part of all modem control systems and network performance will determine many characteristics of a control system. This paper will describe the overall APS network and examine the APS control system network in detail. Metrics are provided on the performance of the system under various conditions

  16. JGrass-NewAge hydrological system: an open-source platform for the replicability of science.

    Science.gov (United States)

    Bancheri, Marialaura; Serafin, Francesco; Formetta, Giuseppe; Rigon, Riccardo; David, Olaf

    2017-04-01

    JGrass-NewAge is an open source semi-distributed hydrological modelling system. It is based on the object modelling framework (OMS version 3), on the JGrasstools and on the Geotools. OMS3 allows to create independent packages of software which can be connected at run-time in a working modelling solution. These components are available as library/dependency or as repository to fork in order to add further features. Different tools are adopted to make easier the integration, the interoperability and the use of each package. Most of the components are Gradle integrated, since it represents the state-of-art of the building systems, especially for Java projects. The continuous integration is a further layer between local source code (client-side) and remote repository (server-side) and ensures the building and the testing of the source code at each commit. Finally, the use of Zenodo makes the code hosted in GitHub unique, citable and traceable, with a defined DOI. Following the previous standards, each part of the hydrological cycle is implemented in JGrass-NewAge as a component that can be selected, adopted, and connected to obtain a user "customized" hydrological model. A variety of modelling solutions are possible, allowing a complete hydrological analysis. Moreover, thanks to the JGrasstools and the Geotools, the visualization of the data and of the results using a selected GIS is possible. After the geomorphological analysis of the watershed, the spatial interpolation of the meteorological inputs can be performed using both deterministic (IDW) and geostatistic (Kriging) algorithms. For the radiation balance, the shortwave and longwave radiation can be estimated, which are, in turn, inputs for the simulation of the evapotranspiration, according to Priestly-Taylor and Penman-Monteith formulas. Three degree-day models are implemented for the snow melting and SWE. The runoff production can be simulated using two different components, "Adige" and "Embedded Reservoirs

  17. Digital control systems. Verteilte Prozessleitsysteme

    Energy Technology Data Exchange (ETDEWEB)

    1984-01-01

    With a distinct description of the systems properties thin regulation shall provide a latter transparency for the use of digital control systems. The application of the new technique shall be facilitated, incitations for the further development shall be given and the compatibility of the systems shall be advanced. Moreover, the regulation can be used as criteria catalogue for the evaluation of digital systems.

  18. Memory B cells and CD8⁺ lymphocytes do not control seasonal influenza A virus replication after homologous re-challenge of rhesus macaques.

    Directory of Open Access Journals (Sweden)

    Timothy D Carroll

    Full Text Available This study sought to define the role of memory lymphocytes in the protection from homologous influenza A virus re-challenge in rhesus macaques. Depleting monoclonal antibodies (mAb were administered to the animals prior to their second experimental inoculation with a human seasonal influenza A virus strain. Treatment with either anti-CD8α or anti-CD20 mAbs prior to re-challenge had minimal effect on influenza A virus replication. Thus, in non-human primates with pre-existing anti-influenza A antibodies, memory B cells and CD8α⁺ T cells do not contribute to the control of virus replication after re-challenge with a homologous strain of influenza A virus.

  19. Controlling systems of cogeneration blocks

    International Nuclear Information System (INIS)

    Suriansky, J.; Suriansky, J. Ml.; Puskajler, J.

    2007-01-01

    In this article the main parts of cogeneration unit control system are described. Article is aimed on electric power measurement with electricity protection as with temperature system regulation. In conclusion of the article, the control algorithm with perspective of cogeneration solve is indicated. (authors)

  20. Implementacija metode automatizovanih nezavisnih ponavljanja u simulaciji sistema masovnog opsluživanja / Implementation of automated independent replications in the simulation of queuing systems

    Directory of Open Access Journals (Sweden)

    Nebojša V. Nikolić

    2010-10-01

    agreement of the model with the real system under study is one of the most important characteristics of the model. This means that a conceptual model should present reality as it really is. In the context of queuing system modeling, this means the following: if a real system works for some finite time, then the same should be applied for a model as well; if there can be any traffic intensity including overloading, then a model should be conceived accordingly, etc. Mathematical basis of the AIRGSSP method The mathematical basis of the method is offered, as well as some recommendations for practical implementation of the method in a form of a computer program algorithm. Explicitly, the mathematical foundations of the AIRGSSP method are three statements; the first one, the Bernoulli law of large numbers, or the first limit theorem: the frequency of one random event in a many times repeated experiment tends to the probability of that event; the second one, the perception of the behavior of a queuing system as a kind of a stochastic process, and the support for the statistics of stochastic processes; and the third one, the use of an interval estimation of proportion for establishing the control of the simulation result accuracy. Description of the implementation of the AIRGSSP method The application of the AIRGSSP method includes the following: development of a conceptual model; definition of performance measures; development of the basic structure of the simulation model; implementation of a module for the stochastic process statistics; implementation of a module for automatization of independent replications of the simulation experiment; calculation of a number of replications of the simulation experiment; and translation of the model into the computer code. Conclusion A detailed description for the practical implementation of the method 'Automated Independent Replications with Gathering Statistics of Stochastic Processes' is given. The capacity and advantages of the AIRGSSP

  1. Automatic Bridge Control System

    OpenAIRE

    M. Niraimathi; S.Sivakumar; R.Vigneshwaran; R.Vinothkumar; P.Babu

    2012-01-01

    Bridge vibration control is an important issue whose purpose is to extend the structural service life of bridges. Normally, the bridge is modeled as an elastic beam or plate subject to a moving vehicle. However, the moving truck on a bridge is a complicated problem that must still be researched. In this paper, wepropose a new method, to overcome the huge load in the bridge a load cell is used at the entry which will monitor the load continuously at both ends. To escape from the heavy water fl...

  2. Virtualization in control system environment

    International Nuclear Information System (INIS)

    Shen, L.R.; Liu, D.K.; Wan, T.M.

    2012-01-01

    In large scale distributed control system, there are lots of common service composed an environment for the entire control system, such as the server system for the common software base library, application server, archive server and so on. This paper gives a description of a virtualization realization for control system environment including the virtualization for server, storage, network system and application for the control system. With a virtualization instance of the EPICS based control system environment that was built by the VMware vSphere v4, we tested the whole functionality of this virtualization environment in the SSRF control system, including the common server of the NFS, NIS, NTP, Boot and EPICS base and extension library tools, we also have applied virtualization to application servers such as the Archive, Alarm, EPICS gateway and all of the network based IOC. Specially, we test the high availability and VMotion for EPICS asynchronous IOC successful under the different VLAN configuration of the current SSRF control system network. (authors)

  3. Sustained Inhibition of HBV Replication In Vivo after Systemic Injection of AAVs Encoding Artificial Antiviral Primary MicroRNAs.

    Science.gov (United States)

    Maepa, Mohube Betty; Ely, Abdullah; Grayson, Wayne; Arbuthnot, Patrick

    2017-06-16

    Chronic infection with hepatitis B virus (HBV) remains a problem of global significance and improving available treatment is important to prevent life-threatening complications arising in persistently infected individuals. HBV is susceptible to silencing by exogenous artificial intermediates of the RNA interference (RNAi) pathway. However, toxicity of Pol III cassettes and short duration of silencing by effectors of the RNAi pathway may limit anti-HBV therapeutic utility. To advance RNAi-based HBV gene silencing, mono- and trimeric artificial primary microRNAs (pri-miRs) derived from pri-miR-31 were placed under control of the liver-specific modified murine transthyretin promoter. The sequences, which target the X sequence of HBV, were incorporated into recombinant hepatotropic self-complementary adeno-associated viruses (scAAVs). Systemic intravenous injection of the vectors into HBV transgenic mice at a dose of 1 × 10 11 per animal effected significant suppression of markers of HBV replication for at least 32 weeks. The pri-miRs were processed according to the intended design, and intrahepatic antiviral guide sequences were detectable for 40 weeks after the injection. There was no evidence of toxicity, and innate immunostimulation was not detectable following the injections. This efficacy is an improvement on previously reported RNAi-based inhibition of HBV replication and is important to clinical translation of the technology. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  4. Optimal Control of Mechanical Systems

    Directory of Open Access Journals (Sweden)

    Vadim Azhmyakov

    2007-01-01

    Full Text Available In the present work, we consider a class of nonlinear optimal control problems, which can be called “optimal control problems in mechanics.” We deal with control systems whose dynamics can be described by a system of Euler-Lagrange or Hamilton equations. Using the variational structure of the solution of the corresponding boundary-value problems, we reduce the initial optimal control problem to an auxiliary problem of multiobjective programming. This technique makes it possible to apply some consistent numerical approximations of a multiobjective optimization problem to the initial optimal control problem. For solving the auxiliary problem, we propose an implementable numerical algorithm.

  5. Docosahexaenoic acid for reading, working memory and behavior in UK children aged 7-9: A randomized controlled trial for replication (the DOLAB II study.

    Directory of Open Access Journals (Sweden)

    Paul Montgomery

    Full Text Available Omega-3 fatty acids are central to brain-development of children. Evidence from clinical trials and systematic reviews demonstrates the potential of long-chain Omega-3 supplementation for learning and behavior. However, findings are inconclusive and in need of robust replication studies since such work is lacking.Replication of the 2012 DOLAB 1 study findings that a dietary supplementation with the long-chain omega-3 docosahexaenoic acid (DHA had beneficial effects on the reading, working memory, and behavior of healthy schoolchildren.Parallel group, fixed-dose, randomized (minimization, 30% random element, double-blind, placebo-controlled trial (RCT.Mainstream primary schools (n = 84 from five counties in the UK in 2012-2015.Healthy children aged 7-9 underperforming in reading (<20th centile. 1230 invited, 376 met study criteria.600 mg/day DHA (from algal oil, placebo: taste/color matched corn/soybean oil; for 16 weeks.Age-standardized measures of reading, working memory, and behavior, parent-rated and as secondary outcome teacher-rated.376 children were randomized. Reading, working memory, and behavior change scores showed no consistent differences between intervention and placebo group. Some behavioral subscales showed minor group differences.This RCT did not replicate results of the earlier DOLAB 1 study on the effectiveness of nutritional supplementation with DHA for learning and behavior. Possible reasons are discussed, particularly regarding the replication of complex interventions.www.controlled-trials.com (ISRCTN48803273 and protocols.io (https://dx.doi.org/10.17504/protocols.io.k8kczuw.

  6. Decentralized control of complex systems

    CERN Document Server

    Siljak, Dragoslav D

    2011-01-01

    Complex systems require fast control action in response to local input, and perturbations dictate the use of decentralized information and control structures. This much-cited reference book explores the approaches to synthesizing control laws under decentralized information structure constraints.Starting with a graph-theoretic framework for structural modeling of complex systems, the text presents results related to robust stabilization via decentralized state feedback. Subsequent chapters explore optimization, output feedback, the manipulative power of graphs, overlapping decompositions and t

  7. Argonne's atlas control system upgrade

    International Nuclear Information System (INIS)

    Munson, F.; Quock, D.; Chapin, B.; Figueroa, J.

    1999-01-01

    The ATLAS facility (Argonne Tandem-Linac Accelerator System) is located at the Argonne National Laboratory. The facility is a tool used in nuclear and atomic physics research, which focuses primarily on heavy-ion physics. The accelerator as well as its control system are evolutionary in nature, and consequently, continue to advance. In 1998 the most recent project to upgrade the ATLAS control system was completed. This paper briefly reviews the upgrade, and summarizes the configuration and features of the resulting control system

  8. PLC VVVF Elevator Control System

    OpenAIRE

    Tang, Yujian; Gui, Tianyu

    2016-01-01

    The aim of the thesis is to introduce the PLC VVVF elevator and its control system. The thesis can be divided into three parts. The first part is about the overview of the lift: the kinds of the lift and the structure of the lift, it shows the knowledge about the components and the operating systems of the lift. The second part is about the PLC control system, it’s about the operations of the lift from the introduction about the hardware and software of the PLC control system. And the thi...

  9. Vacuum control system of VEC

    International Nuclear Information System (INIS)

    Roy, Anindya; Bhole, R.B.; Bandopadhyay, D.L.; Mukhopadhyay, B.; Pal, Sarbajit; Sarkar, D.

    2009-01-01

    As a part of modernization of VEC (Variable Energy Cyclotron), the Vacuum Control System is being upgraded to PLC based automated system from initial Relay based Manual system. EPICS (Experimental Physics and Industrial Control System), a standard open source software tool for designing distributed control system, is chosen for developing the supervisory control software layer, leading towards a unified distributed control architecture of VEC Control System. A Modbus - TCP based IOC (I/O Controller) has been developed to communicate control data to PLC using Ethernet-TCP LAN. Keeping in mind, the operators' familiarity with MS-Windows, a MS-Windows based operator interface is developed using VB6. It is also used to test and evaluate EPICS compatibility to MS Windows. Several MS Windows ActiveX components e.g. text display, image display, alarm window, set-point input etc. have been developed incorporating Channel Access library of EPICS. Use of such components ease the programming complexity and reduce developmental time of the operator interface. The system is in the final phase of commissioning. (author)

  10. Characterizing the Final Steps of Chromosomal Replication at the Single-molecule Level in the Model System Escherichia coli

    KAUST Repository

    Elshenawy, Mohamed

    2015-01-01

    In the circular Escherichia coli chromosome, two replisomes are assembled at the unique origin of replication and drive DNA synthesis in opposite directions until they meet in the terminus region across from the origin. Despite the difference

  11. Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV in macaques vaccinated with replication-deficient viral vectors

    Directory of Open Access Journals (Sweden)

    Strasak Alexander

    2009-06-01

    Full Text Available Abstract Background We investigated the interplay between complement and antibodies upon priming with single-cycle replicating viral vectors (SCIV encoding SIV antigens combined with Adeno5-SIV or SCIV pseudotyped with murine leukemia virus envelope boosting strategies. The vaccine was applied via spray-immunization to the tonsils of rhesus macaques and compared with systemic regimens. Results Independent of the application regimen or route, viral loads were significantly reduced after challenge with SIVmac239 (p Conclusion The heterologous prime-boost strategy with replication-deficient viral vectors administered exclusively via the tonsils did not induce any neutralizing antibodies before challenge. However, after challenge, comparable SIV-specific humoral immune responses were observed in all vaccinated animals. Immunization with single cycle immunodeficiency viruses mounts humoral immune responses comparable to live-attenuated immunodeficiency virus vaccines.

  12. Genome-Wide Search for Competing Endogenous RNAs Responsible for the Effects Induced by Ebola Virus Replication and Transcription Using a trVLP System

    Directory of Open Access Journals (Sweden)

    Zhong-Yi Wang

    2017-11-01

    Full Text Available Understanding how infected cells respond to Ebola virus (EBOV and how this response changes during the process of viral replication and transcription are very important for establishing effective antiviral strategies. In this study, we conducted a genome-wide screen to identify long non-coding RNAs (lncRNAs, circular RNAs (circRNAs, micro RNAs (miRNAs, and mRNAs differentially expressed during replication and transcription using a tetracistronic transcription and replication-competent virus-like particle (trVLP system that models the life cycle of EBOV in 293T cells. To characterize the expression patterns of these differentially expressed RNAs, we performed a series cluster analysis, and up- or down-regulated genes were selected to establish a gene co-expression network. Competing endogenous RNA (ceRNA networks based on the RNAs responsible for the effects induced by EBOV replication and transcription in human cells, including circRNAs, lncRNAs, miRNAs, and mRNAs, were constructed for the first time. Based on these networks, the interaction details of circRNA-chr19 were explored. Our results demonstrated that circRNA-chr19 targeting miR-30b-3p regulated CLDN18 expression by functioning as a ceRNA. These findings may have important implications for further studies of the mechanisms of EBOV replication and transcription. These RNAs potentially have important functions and may be promising targets for EBOV therapy.

  13. Database Replication Prototype

    OpenAIRE

    Vandewall, R.

    2000-01-01

    This report describes the design of a Replication Framework that facilitates the implementation and com-parison of database replication techniques. Furthermore, it discusses the implementation of a Database Replication Prototype and compares the performance measurements of two replication techniques based on the Atomic Broadcast communication primitive: pessimistic active replication and optimistic active replication. The main contributions of this report can be split into four parts....

  14. Mothers in methadone treatment and their involvement with the child protection system: a replication and extension study.

    Science.gov (United States)

    Taplin, Stephanie; Mattick, Richard P

    2013-08-01

    Although a high level of involvement with the child protection system has been identified in families where parental substance use is a feature, not all such parents abuse or neglect their children or have contact with the child protection system. Identifying parents with substance-use histories who are able to care for their children without intervention by the child protection system, and being able to target interventions to the families who need them the most is important. This study interviewed a relatively large sample of mothers about their histories, their children and their involvement with the child protection system. We hypothesized that mothers in opioid pharmacological treatment who are involved with child protection services are different in characteristics to those mothers who are not involved. One hundred and seventy-one women, with at least one child aged under 16 years, were interviewed at nine treatment clinics providing pharmacological treatment for opioid dependence across Sydney, Australia. Just over one-third of the women were involved with child protection services at the time of interview, mostly with children in out-of-home care. Logistic regression analyses revealed that factors which significantly increased the likelihood of the mother being involved with the child protection system were: (1) having a greater number of children, (2) being on psychiatric medication, and (3) having less than daily contact with her own parents. This study replicates and extends the work of Grella, Hser, and Huang (2006) and the limited literature published to date examining the factors which contribute to some substance-using mothers becoming involved with the child protection system while others do not. The finding that mental health problems and parental supports (along with the number of children) were significantly associated with child protection system involvement in this study, indicates a need for improved interventions and the provision of

  15. Genetic Interaction Mapping in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a ROTOR HDA Colony Replicating Robot in a 1536 Array Format.

    Science.gov (United States)

    Roguev, Assen; Xu, Jiewei; Krogan, Nevan

    2018-02-01

    This protocol describes an optimized high-throughput procedure for generating double deletion mutants in Schizosaccharomyces pombe using the colony replicating robot ROTOR HDA and the PEM (pombe epistasis mapper) system. The method is based on generating high-density colony arrays (1536 colonies per agar plate) and passaging them through a series of antidiploid and mating-type selection (ADS-MTS) and double-mutant selection (DMS) steps. Detailed program parameters for each individual replication step are provided. Using this procedure, batches of 25 or more screens can be routinely performed. © 2018 Cold Spring Harbor Laboratory Press.

  16. JT-60 plasma control system

    International Nuclear Information System (INIS)

    Kurihara, K.

    1988-01-01

    JT-60 plasma control can be performed by the supervisory controller, the measurement system and actuators such as the poloidal field coil power supplies, gas injectors, neutral beam injection (NBI) heating system and radio frequency (RF) heating system. One of the most important characteristics of this system is a perfect digital control one composed of mini-computers, fast array processors and CAMAC modules, and it has large flexibility and few troubles to adjust the system. This system started to be operated in April 1985, after the six-year-long design, construction and testing, and have been operated and improved many times for two years. In this paper, the final system specification and its performance are presented aiming at the technological aspect of hardware and software. In addition, and experienced troubles are also presented. (author)

  17. The effects of ozone and water exchange rates on water quality and rainbow trout Oncorhynchus mykiss performance in replicated water recirculating systems

    Science.gov (United States)

    Rainbow trout Oncorhynchus mykiss performance and water quality were evaluated and compared within six replicated 9.5 cubic meter water recirculating aquaculture systems (WRAS) operated with and without ozone at various water exchange rates. Three separate studies were conducted: 1) low water exchan...

  18. Computer control system of TRISTAN

    International Nuclear Information System (INIS)

    Kurokawa, Shin-ichi; Shinomoto, Manabu; Kurihara, Michio; Sakai, Hiroshi.

    1984-01-01

    For the operation of a large accelerator, it is necessary to connect an enormous quantity of electro-magnets, power sources, vacuum equipment, high frequency accelerator and so on and to control them harmoniously. For the purpose, a number of computers are adopted, and connected with a network, in this way, a large computer system for laboratory automation which integrates and controls the whole system is constructed. As a distributed system of large scale, the functions such as electro-magnet control, file processing and operation control are assigned to respective computers, and the total control is made feasible by network connection, at the same time, as the interface with controlled equipment, the CAMAC (computer-aided measurement and control) is adopted to ensure the flexibility and the possibility of expansion of the system. Moreover, the language ''NODAL'' having network support function was developed so as to easily make software without considering the composition of more complex distributed system. The accelerator in the TRISTAN project is composed of an electron linear accelerator, an accumulation ring of 6 GeV and a main ring of 30 GeV. Two ring type accelerators must be synchronously operated as one body, and are controlled with one computer system. The hardware and software are outlined. (Kako, I.)

  19. Control of Solar Energy Systems

    CERN Document Server

    Camacho, Eduardo F; Rubio, Francisco R; Martínez, Diego

    2012-01-01

    Control of Solar Energy Systems details the main solar energy systems, problems involved with their control, and how control systems can help in increasing their efficiency.  After a brief introduction to the fundamental concepts associated with the use of solar energy in both photovoltaic and thermal plants, specific issues related to control of solar systems are embarked upon. Thermal energy systems are then explored in depth, as well as  other solar energy applications such as solar furnaces and solar refrigeration systems. Problems of variable generation profile and of the contribution of many solar plants to the same grid system are considered with the necessary integrated and supervisory control solutions being discussed. The text includes material on: ·         A comparison of basic and advanced control methods for parabolic troughs from PID to nonlinear model-based control; ·         solar towers and solar tracking; ·         heliostat calibration, characterization and off...

  20. LHCb experience with LFC replication

    International Nuclear Information System (INIS)

    Bonifazi, F; Carbone, A; D'Apice, A; Dell'Agnello, L; Re, G L; Martelli, B; Ricci, P P; Sapunenko, V; Vitlacil, D; Perez, E D; Duellmann, D; Girone, M; Peco, G; Vagnoni, V

    2008-01-01

    Database replication is a key topic in the framework of the LHC Computing Grid to allow processing of data in a distributed environment. In particular, the LHCb computing model relies on the LHC File Catalog, i.e. a database which stores information about files spread across the GRID, their logical names and the physical locations of all the replicas. The LHCb computing model requires the LFC to be replicated at Tier-1s. The LCG 3D project deals with the database replication issue and provides a replication service based on Oracle Streams technology. This paper describes the deployment of the LHC File Catalog replication to the INFN National Center for Telematics and Informatics (CNAF) and to other LHCb Tier-1 sites. We performed stress tests designed to evaluate any delay in the propagation of the streams and the scalability of the system. The tests show the robustness of the replica implementation with performance going much beyond the LHCb requirements

  1. LHCb experience with LFC replication

    CERN Document Server

    Bonifazi, F; Perez, E D; D'Apice, A; dell'Agnello, L; Düllmann, D; Girone, M; Re, G L; Martelli, B; Peco, G; Ricci, P P; Sapunenko, V; Vagnoni, V; Vitlacil, D

    2008-01-01

    Database replication is a key topic in the framework of the LHC Computing Grid to allow processing of data in a distributed environment. In particular, the LHCb computing model relies on the LHC File Catalog, i.e. a database which stores information about files spread across the GRID, their logical names and the physical locations of all the replicas. The LHCb computing model requires the LFC to be replicated at Tier-1s. The LCG 3D project deals with the database replication issue and provides a replication service based on Oracle Streams technology. This paper describes the deployment of the LHC File Catalog replication to the INFN National Center for Telematics and Informatics (CNAF) and to other LHCb Tier-1 sites. We performed stress tests designed to evaluate any delay in the propagation of the streams and the scalability of the system. The tests show the robustness of the replica implementation with performance going much beyond the LHCb requirements.

  2. Upgrading the ATLAS control system

    International Nuclear Information System (INIS)

    Munson, F.H.; Ferraretto, M.

    1993-01-01

    Heavy-ion accelerators are tools used in the research of nuclear and atomic physics. The ATLAS facility at the Argonne National Laboratory is one such tool. The ATLAS control system serves as the primary operator interface to the accelerator. A project to upgrade the control system is presently in progress. Since this is an upgrade project and not a new installation, it was imperative that the development work proceed without interference to normal operations. An additional criteria for the development work was that the writing of additional ''in-house'' software should be kept to a minimum. This paper briefly describes the control system being upgraded, and explains some of the reasons for the decision to upgrade the control system. Design considerations and goals for the new system are described, and the present status of the upgrade is discussed

  3. Control Evaluation Information System Savings

    Directory of Open Access Journals (Sweden)

    Eddy Sutedjo

    2011-05-01

    Full Text Available The purpose of this research is to evaluate the control of information system savings in the banking and to identify the weaknesses and problem happened in those saving systems. Research method used are book studies by collecting data and information needed and field studies by interview, observation, questioner, and checklist using COBIT method as a standard to assess the information system control of the company. The expected result about the evaluation result that show in the problem happened and recommendation given as the evaluation report and to give a view about the control done by the company. Conclusion took from this research that this banking company has met standards although some weaknesses still exists in the system.Index Terms - Control Information System, Savings

  4. Computational system to create an entry file for replicating I-125 seeds simulating brachytherapy case studies using the MCNPX code

    Directory of Open Access Journals (Sweden)

    Leonardo da Silva Boia

    2014-03-01

    decline for short distances.------------------------------Cite this article as: Boia LS, Junior J, Menezes AF, Silva AX. Computational system to create an entry file for replicating I-125 seeds simulating brachytherapy case studies using the MCNPX code. Int J Cancer Ther Oncol 2014; 2(2:02023.DOI: http://dx.doi.org/10.14319/ijcto.0202.3

  5. Manual control of unstable systems

    Science.gov (United States)

    Allen, R. W.; Hogue, J. R.; Parseghian, Z.

    1986-01-01

    Under certain operational regimes and failure modes, air and ground vehicles can present the human operator with a dynamically unstable or divergent control task. Research conducted over the last two decades has explored the ability of the human operator to control unstable systems under a variety of circumstances. Past research is reviewed and human operator control capabilities are summarized. A current example of automobile directional control under rear brake lockup conditions is also reviewed. A control system model analysis of the driver's steering control task is summarized, based on a generic driver/vehicle model presented at last year's Annual Manual. Results from closed course braking tests are presented that confirm the difficulty the average driver has in controlling the unstable directional dynamics arising from rear wheel lockup.

  6. Cigarette weight control systems

    International Nuclear Information System (INIS)

    Powell, G.F.W.; Bolt, R.C.; Simmons, A.

    1980-01-01

    A system is described for monitoring the weight of a continuous wrapped rod of tobacco formed by a cigarette-making machine. A scanner unit can be used which passes beta-rays from a primary radiation source through the rod. The absorption is measured by comparison of the intensity at a detector on the opposite side of the rod with that at a detector facing another smaller source, the balance unit. This is pre-set so that when the rod weight is correct the detected intensities from the two sources will be equal. It is essential that the scanning station is kept clean otherwise the dust is included in the weight reading and the cigarettes manufactured would be underweight. This can be checked using an artificial cigarette of known weight as a calibration check. In this device a test circuit can be connected to the scanner head and this opens the shutter over the radioactive source when the test is initiated. A warning device is initiated if the reading is beyond predetermined limits and can be made to prevent operation of the cigarette machine if a satisfactory test is not obtained. (U.K.)

  7. Vehicle electrical system state controller

    Science.gov (United States)

    Bissontz, Jay E.

    2017-10-17

    A motor vehicle electrical power distribution system includes a plurality of distribution sub-systems, an electrical power storage sub-system and a plurality of switching devices for selective connection of elements of and loads on the power distribution system to the electrical power storage sub-system. A state transition initiator provides inputs to control system operation of switching devices to change the states of the power distribution system. The state transition initiator has a plurality of positions selection of which can initiate a state transition. The state transition initiator can emulate a four position rotary ignition switch. Fail safe power cutoff switches provide high voltage switching device protection.

  8. ISABELLE control system: design concepts

    International Nuclear Information System (INIS)

    Humphrey, J.W.

    1979-01-01

    ISABELLE is a Department of Energy funded proton accelerator/storage ring being built at Brookhaven National Laboratory (Upton, Long Island, New York). It is large (3.8 km circumference) and complicated (approx. 30,000 monitor and control variables). It is based on superconducting technology. Following the example of previous accelerators, ISABELLE will be operated from a single control center. The control system will be distributed and will incorporate a local computer network. An overview of the conceptual design of the ISABELLE control system will be presented

  9. Traction Control System for Motorcycles

    Directory of Open Access Journals (Sweden)

    Cardinale Pascal

    2009-01-01

    Full Text Available Traction control is a widely used control system to increase stability and safety of four wheel vehicles. Automatic stability control is used in the BMW K1200R motorcycle and in motoGP competition, but not in other motorcycles. This paper presents an algorithm and a low-cost real-time hardware implementation for motorcycles. A prototype has been developed, applied on a commercial motorcycle, and tested in a real track. The control system that can be tuned by the driver during the race has been appreciated by the test driver.

  10. Controllability of a multichannel system

    Science.gov (United States)

    Ivanov, Sergei A.; Wang, Jun Min

    2018-02-01

    We consider the system consisting of K coupled acoustic channels with the different sound velocities cj. Channels are interacting at any point via the pressure and its time derivatives. Using the moment approach and the theory of exponential families with vector coefficients we establish two controllability results: the system is exactly controllable if (i) the control uj in the jth channel acts longer than the double travel time of a wave from the start to the end of the j-th channel; (ii) all controls uj act more than or equal to the maximal double travel time.

  11. Test results of HTTR control system

    International Nuclear Information System (INIS)

    Motegi, Toshihiro; Iigaki, Kazuhiko; Saito, Kenji; Sawahata, Hiroaki; Hirato, Yoji; Kondo, Makoto; Shibutani, Hideki; Ogawa, Satoru; Shinozaki, Masayuki; Mizushima, Toshihiko; Kawasaki, Kozo

    2006-06-01

    The plant control performance of the IHX helium flow rate control system, the PPWC helium flow rate control system, the secondary helium flow rate control system, the inlet temperature control system, the reactor power control system and the outlet temperature control system of the HTTR are obtained through function tests and power-up tests. As the test results, the control systems show stable control response under transient condition. Both of inlet temperature control system and reactor power control system shows stable operation from 30% to 100%, respectively. This report describes the outline of control systems and test results. (author)

  12. MULTIPLE ECH LAUNCHER CONTROL SYSTEM

    International Nuclear Information System (INIS)

    GREEN, M.T.; PONCE, D.; GRUNLOH, H.J.; ELLIS, R.A.; GROSNICKLE, W.H.; HUMPHREY, R.L.

    2004-03-01

    OAK-B135 The addition of new, high power gyrotrons to the heating and current drive arsenal at DIII-D, required a system upgrade for control of fully steerable ECH Launchers. Each launcher contains two pointing mirrors with two degrees of mechanical freedom. The two flavors of motion are called facet and tilt. Therefore up to four channels of motion per launcher need to be controlled. The system utilizes absolute encoders to indicate mirror position and therefore direction of the microwave beam. The launcher movement is primarily controlled by PLC, but future iterations of design, may require this control to be accomplished by a CPU on fast bus such as Compact PCI. This will be necessary to accomplish real time position control. Safety of equipment and personnel is of primary importance when controlling a system of moving parts. Therefore multiple interlocks and fault status enunciators have been implemented. This paper addresses the design of a Multiple ECH Launcher Control System, and characterizes the flexibility needed to upgrade to a real time position control system in the future

  13. Weld analysis and control system

    Science.gov (United States)

    Kennedy, Larry Z. (Inventor); Rodgers, Michael H. (Inventor); Powell, Bradley W. (Inventor); Burroughs, Ivan A. (Inventor); Goode, K. Wayne (Inventor)

    1994-01-01

    The invention is a Weld Analysis and Control System developed for active weld system control through real time weld data acquisition. Closed-loop control is based on analysis of weld system parameters and weld geometry. The system is adapted for use with automated welding apparatus having a weld controller which is capable of active electronic control of all aspects of a welding operation. Enhanced graphics and data displays are provided for post-weld analysis. The system provides parameter acquisition, including seam location which is acquired for active torch cross-seam positioning. Torch stand-off is also monitored for control. Weld bead and parent surface geometrical parameters are acquired as an indication of weld quality. These parameters include mismatch, peaking, undercut, underfill, crown height, weld width, puddle diameter, and other measurable information about the weld puddle regions, such as puddle symmetry, etc. These parameters provide a basis for active control as well as post-weld quality analysis and verification. Weld system parameters, such as voltage, current and wire feed rate, are also monitored and archived for correlation with quality parameters.

  14. The BATES linac control system

    International Nuclear Information System (INIS)

    Russ, T.; Radouch, Z.

    1989-01-01

    The Bates linac control system (LCS), a distributed processing architecture, is described. Due to the historic evolution of the system, a mix of different hardware, operating systems and programming languages are used throughout. However, a standardized interface at the network level enables a smooth system integration. In particular, a multicasting scheme for data transmission over the network permits simultaneous database updates on more than one workstation. This allows for true distribution of data processing power. 3 figs

  15. Packaging of control system software

    International Nuclear Information System (INIS)

    Zagar, K.; Kobal, M.; Saje, N.; Zagar, A.; Sabjan, R.; Di Maio, F.; Stepanov, D.

    2012-01-01

    Control system software consists of several parts - the core of the control system, drivers for integration of devices, configuration for user interfaces, alarm system, etc. Once the software is developed and configured, it must be installed to computers where it runs. Usually, it is installed on an operating system whose services it needs, and also in some cases dynamically links with the libraries it provides. Operating system can be quite complex itself - for example, a typical Linux distribution consists of several thousand packages. To manage this complexity, we have decided to rely on Red Hat Package Management system (RPM) to package control system software, and also ensure it is properly installed (i.e., that dependencies are also installed, and that scripts are run after installation if any additional actions need to be performed). As dozens of RPM packages need to be prepared, we are reducing the amount of effort and improving consistency between packages through a Maven-based infrastructure that assists in packaging (e.g., automated generation of RPM SPEC files, including automated identification of dependencies). So far, we have used it to package EPICS, Control System Studio (CSS) and several device drivers. We perform extensive testing on Red Hat Enterprise Linux 5.5, but we have also verified that packaging works on CentOS and Scientific Linux. In this article, we describe in greater detail the systematic system of packaging we are using, and its particular application for the ITER CODAC Core System. (authors)

  16. Display systems for NPP control

    International Nuclear Information System (INIS)

    Rozov, S.S.

    1988-01-01

    Main trends in development of display systems used as the means for image displaying in NPP control systems are considered. It is shown that colour display devices appear to be the most universal means for concentrated data presentation. Along with digital means the display systems provide for high-speed response, sufficient for operative control of executive mechanisms. A conclusion is drawn that further development of display systems will move towards creation of large colour fields (on reflection base or with multicolour gas-discharge elements)

  17. The AGS Booster control system

    International Nuclear Information System (INIS)

    Frankel, R.; Auerbach, E.; Culwick, B.; Clifford, T.; Mandell, S.; Mariotti, R.; Salwen, C.; Schumburg, N.

    1988-01-01

    Although moderate in size, the Booster construction project requires a comprehensive control system. There are three operational modes: as a high intensity proton injector for the AGS, as a heavy ion accelerator and injector supporting a wide range of ions and as a polarized proton storage injector. These requirements are met using a workstation based extension of the existing AGS control system. Since the Booster is joining a complex of existing accelerators, the new system will be capable of supporting multiuser operational scenarios. A short discussion of this system is discussed in this paper

  18. VA National Bed Control System

    Data.gov (United States)

    Department of Veterans Affairs — The VA National Bed Control System records the levels of operating, unavailable and authorized beds at each VAMC, and it tracks requests for changes in these levels....

  19. Once in a lifetime: strategies for preventing re-replication in prokaryotic and eukaryotic cells

    DEFF Research Database (Denmark)

    Nielsen, Olaf; Løbner-Olesen, Anders

    2008-01-01

    DNA replication is an extremely accurate process and cells have evolved intricate control mechanisms to ensure that each region of their genome is replicated only once during S phase. Here, we compare what is known about the processes that prevent re-replication in prokaryotic and eukaryotic cells...... prokaryotes and eukaryotes are inactivated until the next cell cycle. Furthermore, in both systems the beta-clamp of the replicative polymerase associates with enzymatic activities that contribute to the inactivation of the helicase loaders. Finally, recent studies suggest that the control mechanism...

  20. Control mechanisms in franchise systems

    OpenAIRE

    Hass, Jörg

    2012-01-01

    This dissertation answers the question which different control mechanisms exist in a franchise system. It is the first two-sided franchise empirical analysis, regarding all outlets of the franchise system (franchisees and company-owned) as well as the franchisor. On the theoretical side, this dissertation integrates the two main management theories: principal-agent-theory and transaction cost analysis. The results show that there are used different control mechanisms in a franchise sys...

  1. A cleanroom contamination control system

    OpenAIRE

    Whyte, W.; Eaton, T.

    2002-01-01

    Analytical methods for hazard and risk analysis are being considered for controlling contamination\\ud in pharmaceutical cleanrooms. The most suitable method appears to be the HACCP system that has\\ud been developed for the food industry, but this requires some reinterpretation for use in\\ud pharmaceutical manufacturing. This paper suggests a possible system.\\ud To control contamination effectively, it is necessary to have a good appreciation of the routes and\\ud sources of contamination, and ...

  2. Nova target diagnostics control system

    International Nuclear Information System (INIS)

    Severyn, J.R.

    1985-01-01

    During the past year the Nova target diagnostics control system was finished and put in service. The diagnostics loft constructed to the north of the target room provides the environmental conditions required to collect reliable target diagnostic data. These improvements include equipment cooling and isolation of the power source with strict control of instrumentation grounds to eliminate data corruption due to electromagnetic pulses from the laser power-conditioning system or from target implosion effects

  3. The ILC global control system

    International Nuclear Information System (INIS)

    Carwardine, J.; Arnold, N.; Lenkszus, F.; Saunders, C.; Rehlich, K.; Simrock, S.; Banerjee, B.; Chase, B.; Gottschalk, E.; Joireman, P.; Kasley, P.; Lackey, S.; McBride, P.; Pavlicek, V.; Patrick, J.; Votava, M.; Wolbers, S.; Furukawa, K.; Michizono, S.; Larsen, R.S.; Downing, R.

    2008-01-01

    The scale and performance parameters of the ILC require new thinking in regards to control system design. This design work has begun quite early in comparison to most accelerator projects, with the goal of uniquely high overall accelerator availability. Among the design challenges are high control system availability, precision timing and rf phase reference distribution, standardizing of interfaces, operability, and maintainability. We present the current state of the design and take a prospective look at ongoing research and development projects.

  4. Elements in the canine distemper virus M 3' UTR contribute to control of replication efficiency and virulence.

    Directory of Open Access Journals (Sweden)

    Danielle E Anderson

    Full Text Available Canine distemper virus (CDV is a negative-sense, single-stranded RNA virus within the genus Morbillivirus and the family Paramyxoviridae. The Morbillivirus genome is composed of six transcriptional units that are separated by untranslated regions (UTRs, which are relatively uniform in length, with the exception of the UTR between the matrix (M and fusion (F genes. This UTR is at least three times longer and in the case of CDV also highly variable. Exchange of the M-F region between different CDV strains did not affect virulence or disease phenotype, demonstrating that this region is functionally interchangeable. Viruses carrying the deletions in the M 3' UTR replicated more efficiently, which correlated with a reduction of virulence, suggesting that overall length as well as specific sequence motifs distributed throughout the region contribute to virulence.

  5. Synthesis of pneumatic controll systems

    Directory of Open Access Journals (Sweden)

    D. Nowak

    2011-04-01

    Full Text Available Currently, the basic tool for automating the production processes are the PLCs. However, in many areas application of the pneumaticcontrol systems may be more reasonable. The main factor determining choice of the control technology are costs. In the case of pneumaticsystems, the costs shall be determined by the number of elements used. Therefore, during the design works it is important to choose anappropriate method for the pneumatic control systems synthesis. The article presents the MTS method, which may be used for a discretetechnological processes modeling and PLC programming, as well as for a pneumatic control systems designing. An important element ofthe MTS method is the network of actions, which graphically presents an algorithm of the implemented process. Based on the actionnetwork and operating machine’s functional diagram, the diagram of different states is determinated, which graphically shows changes ofthe control system’s input and output signals. Analysis of the diagram of different states, makes it easy to determine a schematic equation, which shall be the basis for the control system implementation. Advantage of the MTS method is the lack of restrictions on the number of the control system’s input and output signals. The resulting solution is characterized by a minimum number of elements needed to implement the control system.

  6. NPL superconducting Linac control system

    International Nuclear Information System (INIS)

    Swanson, H.E.; Howe, M.A.; Jackson, L.W.; LaCroix, J.M.; Readdy, H.P.; Storm, D.W.; Van Houten, L.P.

    1985-01-01

    The control system for the NPL Linac is based on a Microvax II host computer connected in a star network with 9 satellite computers. These satellites use single board varsions of DEC's PDP 11 processor. The operator's console uses high performance graphics and touch screen technology to display the current linac status and as the means for interactively controlling the operation of the accelerator

  7. Contamination Control: a systems approach

    NARCIS (Netherlands)

    Donck, J.C.J. van der

    2010-01-01

    Contamination influences a wide variety of industrial processes. For complex systems, contamination control, the collective effort to control contamination to such a level that it guarantees or even improves process or product functionality, offers a way for finding workable solutions. Central in

  8. INFORMATION SYSTEM QUALITY CONTROL KNOWLEDGE

    Directory of Open Access Journals (Sweden)

    Vladimir Nikolaevich Babeshko

    2017-02-01

    Full Text Available The development of the educational system is associated with the need to control the quality of educational services. Quality control knowledge is an important part of the scientific process. The penetration of computers into all areas of activities changing approaches and technologies that previously they were used.

  9. Control system oriented human interface

    International Nuclear Information System (INIS)

    Barale, P.; Jacobson, V.; Kilgore, R.; Rondeau, D.

    1976-11-01

    The on-line control system interface for magnet beam steering and focusing in the Bevalac is described. An Aydin model 5205B display generator was chosen. This display generator will allow the computer to completely rewrite a monitor screen in less than 50 ms and is also capable of controlling a color monitor

  10. Robust power system frequency control

    CERN Document Server

    Bevrani, Hassan

    2014-01-01

    This updated edition of the industry standard reference on power system frequency control provides practical, systematic and flexible algorithms for regulating load frequency, offering new solutions to the technical challenges introduced by the escalating role of distributed generation and renewable energy sources in smart electric grids. The author emphasizes the physical constraints and practical engineering issues related to frequency in a deregulated environment, while fostering a conceptual understanding of frequency regulation and robust control techniques. The resulting control strategi

  11. The ATLAS Detector Control System

    CERN Document Server

    Schlenker, S; Kersten, S; Hirschbuehl, D; Braun, H; Poblaguev, A; Oliveira Damazio, D; Talyshev, A; Zimmermann, S; Franz, S; Gutzwiller, O; Hartert, J; Mindur, B; Tsarouchas, CA; Caforio, D; Sbarra, C; Olszowska, J; Hajduk, Z; Banas, E; Wynne, B; Robichaud-Veronneau, A; Nemecek, S; Thompson, PD; Mandic, I; Deliyergiyev, M; Polini, A; Kovalenko, S; Khomutnikov, V; Filimonov, V; Bindi, M; Stanecka, E; Martin, T; Lantzsch, K; Hoffmann, D; Huber, J; Mountricha, E; Santos, HF; Ribeiro, G; Barillari, T; Habring, J; Arabidze, G; Boterenbrood, H; Hart, R; Marques Vinagre, F; Lafarguette, P; Tartarelli, GF; Nagai, K; D'Auria, S; Chekulaev, S; Phillips, P; Ertel, E; Brenner, R; Leontsinis, S; Mitrevski, J; Grassi, V; Karakostas, K; Iakovidis, G.; Marchese, F; Aielli, G

    2011-01-01

    The ATLAS experiment is one of the multi-purpose experiments at the Large Hadron Collider (LHC), constructed to study elementary particle interactions in collisions of high-energy proton beams. Twelve different sub-detectors as well as the common experimental infrastructure are supervised by the Detector Control System (DCS). The DCS enables equipment supervision of all ATLAS sub-detectors by using a system of >130 server machines running the industrial SCADA product PVSS. This highly distributed system reads, processes and archives of the order of 106 operational parameters. Higher level control system layers allow for automatic control procedures, efficient error recognition and handling, and manage the communication with external systems such as the LHC. This contribution firstly describes the status of the ATLAS DCS and the experience gained during the LHC commissioning and the first physics data taking operation period. Secondly, the future evolution and maintenance constraints for the coming years an...

  12. IMPULSE CONTROL HYBRID ELECTRICAL SYSTEM

    Directory of Open Access Journals (Sweden)

    A. A. Lobaty

    2016-01-01

    Full Text Available This paper extends the recently introduced approach for modeling and solving the optimal control problem of fixedswitched mode DC-DC power converter. DCDC converters are a class of electric power circuits that used extensively in regulated DC power supplies, DC motor drives of different types, in Photovoltaic Station energy conversion and other applications due to its advantageous features in terms of size, weight and reliable performance. The main problem in controlling this type converters is in their hybrid nature as the switched circuit topology entails different modes of operation, each of it with its own associated linear continuous-time dynamics.This paper analyses the modeling and controller synthesis of the fixed-frequency buck DC-DC converter, in which the transistor switch is operated by a pulse sequence with constant frequency. In this case the regulation of the DC component of the output voltage is via the duty cycle. The optimization of the control system is based on the formation of the control signal at the output.It is proposed to solve the problem of optimal control of a hybrid system based on the formation of the control signal at the output of the controller, which minimizes a given functional integral quality, which is regarded as a linear quadratic Letov-Kalman functional. Search method of optimal control depends on the type of mathematical model of control object. In this case, we consider a linear deterministic model of the control system, which is common for the majority of hybrid electrical systems. For this formulation of the optimal control problem of search is a problem of analytical design of optimal controller, which has the analytical solution.As an example of the hybrid system is considered a step-down switching DC-DC converter, which is widely used in various electrical systems: as an uninterruptible power supply, battery charger for electric vehicles, the inverter in solar photovoltaic power plants.. A

  13. Supraphysiologic control over HIV-1 replication mediated by CD8 T cells expressing a re-engineered CD4-based chimeric antigen receptor.

    Directory of Open Access Journals (Sweden)

    Rachel S Leibman

    2017-10-01

    Full Text Available HIV is adept at avoiding naturally generated T cell responses; therefore, there is a need to develop HIV-specific T cells with greater potency for use in HIV cure strategies. Starting with a CD4-based chimeric antigen receptor (CAR that was previously used without toxicity in clinical trials, we optimized the vector backbone, promoter, HIV targeting moiety, and transmembrane and signaling domains to determine which components augmented the ability of T cells to control HIV replication. This re-engineered CAR was at least 50-fold more potent in vitro at controlling HIV replication than the original CD4 CAR, or a TCR-based approach, and substantially better than broadly neutralizing antibody-based CARs. A humanized mouse model of HIV infection demonstrated that T cells expressing optimized CARs were superior at expanding in response to antigen, protecting CD4 T cells from infection, and reducing viral loads compared to T cells expressing the original, clinical trial CAR. Moreover, in a humanized mouse model of HIV treatment, CD4 CAR T cells containing the 4-1BB costimulatory domain controlled HIV spread after ART removal better than analogous CAR T cells containing the CD28 costimulatory domain. Together, these data indicate that potent HIV-specific T cells can be generated using improved CAR design and that CAR T cells could be important components of an HIV cure strategy.

  14. The effect of antiretroviral intensification with dolutegravir on residual virus replication in HIV-infected individuals: a randomised, placebo-controlled, double-blind trial.

    Science.gov (United States)

    Rasmussen, Thomas A; McMahon, James H; Chang, J Judy; Audsley, Jennifer; Rhodes, Ajantha; Tennakoon, Surekha; Dantanarayana, Ashanti; Spelman, Tim; Schmidt, Tina; Kent, Stephen J; Morcilla, Vincent; Palmer, Sarah; Elliott, Julian H; Lewin, Sharon R

    2018-04-06

    Whether ongoing virus replication occurs in HIV-infected individuals on antiretroviral therapy (ART) is unclear; therefore, whether residual virus replication is a barrier to achieving a cure for HIV is also unknown. We aimed to establish whether ART intensification with dolutegravir would reveal or affect residual virus replication in HIV-infected individuals on suppressive treatment. In this randomised, placebo-controlled, double-blind trial, we enrolled HIV-infected adults (aged 18 years and older) receiving combination ART (at least three agents) for at least 3 years from the Alfred Hospital and Melbourne Sexual Health Centre, Melbourne, VIC, Australia. Eligible participants had fewer than 50 copies per mL HIV-1 plasma RNA for more than 3 years and fewer than 20 copies per mL at screening and two CD4 counts higher than 350 cells per μL in the previous 24 months including screening. Participants were randomly assigned (1:1) to receive 50 mg oral dolutegravir or placebo once a day for 56 days in addition to background ART. Follow-up was done at days 1, 3, 7, 14, 28, 56, and 84. The primary outcome was the change from baseline in frequency of 2-long terminal repeat (2-LTR) circles in peripheral blood CD4 cells at day 7. This trial is registered with ClinicalTrials.gov, number NCT02500446. Between Sept 21, 2015, and Sept 19, 2016, 46 individuals were screened for inclusion. 40 were eligible for inclusion and were randomly assigned to the dolutegravir (n=21) or placebo group (n=19). All enrolled participants completed the study procedures and no individuals were lost to follow up. All participants were on suppressive ART with 12% receiving protease inhibitors and the others non-nucleoside reverse transcriptase inhibitors. Median 2-LTR circles fold-change from baseline to day 7 was -0·17 (IQR -0·90 to 0·90) in the dolutegravir group and -0·26 (-1·00 to 1·17) in the placebo group (p=0·17). The addition of dolutegravir to pre-existing ART regimens was safe and

  15. Commutated automatic gain control system

    Science.gov (United States)

    Yost, S. R.

    1982-01-01

    A commutated automatic gain control (AGC) system was designed and built for a prototype Loran C receiver. The receiver uses a microcomputer to control a memory aided phase-locked loop (MAPLL). The microcomputer also controls the input/output, latitude/longitude conversion, and the recently added AGC system. The circuit designed for the AGC is described, and bench and flight test results are presented. The AGC circuit described actually samples starting at a point 40 microseconds after a zero crossing determined by the software lock pulse ultimately generated by a 30 microsecond delay and add network in the receiver front end envelope detector.

  16. Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle.

    Science.gov (United States)

    Nishitsuji, Hironori; Yamamoto, Hiromi; Shiina, Ritsuko; Harada, Keisuke; Ujino, Saneyuki; Shimotohno, Kunitada

    2017-02-01

    Currently, it is possible to construct recombinant forms of various viruses, such as human immunodeficiency virus 1 (HIV-1) and hepatitis C virus (HCV), that carry foreign genes such as a reporter or marker protein in their genomes. These recombinant viruses usually faithfully mimic the life cycle of the original virus in infected cells and exhibit the same host range dependence. The development of a recombinant virus enables the efficient screening of inhibitors and the identification of specific host factors. However, to date the construction of recombinant hepatitis B virus (HBV) has been difficult because of various experimental limitations. The main limitation is the compact genome size of HBV, and a fairly strict genome size that does not exceed 1.3 genome sizes, that must be packaged into virions. Thus, the size of a foreign gene to be inserted should be smaller than 0.4 kb if no deletion of the genome DNA is to be performed. Therefore, to overcome this size limitation, the deletion of some HBV DNA is required. Here, we report the construction of recombinant HBV encoding a reporter gene to monitor the early stage of the HBV replication cycle by replacing part of the HBV core-coding region with the reporter gene by deleting part of the HBV pol coding region. Detection of recombinant HBV infection, monitored by the reporter activity, was highly sensitive and less expensive than detection using the currently available conventional methods to evaluate HBV infection. This system will be useful for a number of applications including high-throughput screening for the identification of anti-HBV inhibitors, host factors and virus-susceptible cells.

  17. Computer controlled high voltage system

    Energy Technology Data Exchange (ETDEWEB)

    Kunov, B; Georgiev, G; Dimitrov, L [and others

    1996-12-31

    A multichannel computer controlled high-voltage power supply system is developed. The basic technical parameters of the system are: output voltage -100-3000 V, output current - 0-3 mA, maximum number of channels in one crate - 78. 3 refs.

  18. Nova laser alignment control system

    International Nuclear Information System (INIS)

    Van Arsdall, P.J.; Holloway, F.W.; McGuigan, D.L.; Shelton, R.T.

    1984-01-01

    Alignment of the Nova laser requires control of hundreds of optical components in the ten beam paths. Extensive application of computer technology makes daily alignment practical. The control system is designed in a manner which provides both centralized and local manual operator controls integrated with automatic closed loop alignment. Menudriven operator consoles using high resolution color graphics displays overlaid with transport touch panels allow laser personnel to interact efficiently with the computer system. Automatic alignment is accomplished by using image analysis techniques to determine beam references points from video images acquired along the laser chain. A major goal of the design is to contribute substantially to rapid experimental turnaround and consistent alignment results. This paper describes the computer-based control structure and the software methods developed for aligning this large laser system

  19. STAT2 Knockout Syrian Hamsters Support Enhanced Replication and Pathogenicity of Human Adenovirus, Revealing an Important Role of Type I Interferon Response in Viral Control.

    Directory of Open Access Journals (Sweden)

    Karoly Toth

    2015-08-01

    Full Text Available Human adenoviruses have been studied extensively in cell culture and have been a model for studies in molecular, cellular, and medical biology. However, much less is known about adenovirus replication and pathogenesis in vivo in a permissive host because of the lack of an adequate animal model. Presently, the most frequently used permissive immunocompetent animal model for human adenovirus infection is the Syrian hamster. Species C human adenoviruses replicate in these animals and cause pathology that is similar to that seen with humans. Here, we report findings with a new Syrian hamster strain in which the STAT2 gene was functionally knocked out by site-specific gene targeting. Adenovirus-infected STAT2 knockout hamsters demonstrated an accentuated pathology compared to the wild-type control animals, and the virus load in the organs of STAT2 knockout animals was 100- to 1000-fold higher than that in wild-type hamsters. Notably, the adaptive immune response to adenovirus is not adversely affected in STAT2 knockout hamsters, and surviving hamsters cleared the infection by 7 to 10 days post challenge. We show that the Type I interferon pathway is disrupted in these hamsters, revealing the critical role of interferon-stimulated genes in controlling adenovirus infection. This is the first study to report findings with a genetically modified Syrian hamster infected with a virus. Further, this is the first study to show that the Type I interferon pathway plays a role in inhibiting human adenovirus replication in a permissive animal model. Besides providing an insight into adenovirus infection in humans, our results are also interesting from the perspective of the animal model: STAT2 knockout Syrian hamster may also be an important animal model for studying other viral infections, including Ebola-, hanta-, and dengue viruses, where Type I interferon-mediated innate immunity prevents wild type hamsters from being effectively infected to be used as

  20. Space construction base control system

    Science.gov (United States)

    1978-01-01

    Aspects of an attitude control system were studied and developed for a large space base that is structurally flexible and whose mass properties change rather dramatically during its orbital lifetime. Topics of discussion include the following: (1) space base orbital pointing and maneuvering; (2) angular momentum sizing of actuators; (3) momentum desaturation selection and sizing; (4) multilevel control technique applied to configuration one; (5) one-dimensional model simulation; (6) N-body discrete coordinate simulation; (7) structural analysis math model formulation; and (8) discussion of control problems and control methods.

  1. Replication of micro and nano surface geometries

    DEFF Research Database (Denmark)

    Hansen, Hans Nørgaard; Hocken, R.J.; Tosello, Guido

    2011-01-01

    The paper describes the state-of-the-art in replication of surface texture and topography at micro and nano scale. The description includes replication of surfaces in polymers, metals and glass. Three different main technological areas enabled by surface replication processes are presented......: manufacture of net-shape micro/nano surfaces, tooling (i.e. master making), and surface quality control (metrology, inspection). Replication processes and methods as well as the metrology of surfaces to determine the degree of replication are presented and classified. Examples from various application areas...... are given including replication for surface texture measurements, surface roughness standards, manufacture of micro and nano structured functional surfaces, replicated surfaces for optical applications (e.g. optical gratings), and process chains based on combinations of repeated surface replication steps....

  2. Safety implications of control systems

    International Nuclear Information System (INIS)

    Smith, O.L.

    1983-01-01

    The Safety Implications of Control Systems Program has three major activities in support of USI-A47. The first task is a failure mode and effects analysis of all plant systems which may potentially induce control system disturbance that have safety implications. This task has made a preliminary study of overfill events and recommended cases for further analysis on the hybrid simulator. Work continues on overcooling and undercooling. A detailed investigation of electric power network is in progress. LERs are providing guidance on important failure modes that will provide initial conditions for further simulator studies. The simulator taks is generating a detailed model of the control system supported by appropriate neutronics, hydraulics, and thermodynamics submodels of all other principal plant components. The simulator is in the last stages of development. Checkout calculations are in progress to establish model stability, robustness, and qualitative credibility. Verification against benchmark codes and plant data will follow

  3. New Main Ring control system

    International Nuclear Information System (INIS)

    Seino, K.; Anderson, L.; Ducar, R.; Franck, A.; Gomilar, J.; Hendricks, B.; Smedinghoff, J.

    1990-03-01

    The Fermilab Main Ring control system has been operational for over sixteen years. Aging and obsolescence of the equipment make the maintenance difficult. Since the advent of the Tevatron, considerable upgrades have been made to the controls of all the Fermilab accelerators except the Main Ring. Modernization of the equipment and standardization of the hardware and software have thus become inevitable. The Tevatron CAMAC serial system has been chosen as a basic foundation in order to make the Main Ring control system compatible with the rest of the accelerator complex. New hardware pieces including intelligent CAMAC modules have been designed to satisfy unique requirements. Fiber optic cable and repeaters have been installed in order to accommodate new channel requirements onto the already saturated communication medium system. 8 refs., 2 figs

  4. The AFP Detector Control System

    CERN Document Server

    AUTHOR|(INSPIRE)INSPIRE-00514541; The ATLAS collaboration

    2017-01-01

    The ATLAS Forward Proton (AFP) detector is one of the forward detectors of the ATLAS experiment at CERN aiming at measuring momenta and angles of diffractively scattered protons. Silicon Tracking and Time-of-Flight detectors are located inside Roman Pot stations inserted into beam pipe aperture. The AFP detector is composed of two stations on each side of the ATLAS interaction point and is under commissioning. The detector is provided with high and low voltage distribution systems. Each station has vacuum and cooling systems, movement control and all the required electronics for signal processing. Monitoring of environmental parameters, like temperature and radiation, is also available. The Detector Control System (DCS) provides control and monitoring of the detector hardware and ensures the safe and reliable operation of the detector, assuring good data quality. Comparing with DCS systems of other detectors, the AFP DCS main challenge is to cope with the large variety of AFP equipment. This paper describes t...

  5. The AFP detector control system

    CERN Document Server

    AUTHOR|(INSPIRE)INSPIRE-00514541; The ATLAS collaboration; Caforio, Davide; Czekierda, Sabina; Hajduk, Zbigniew; Olszowska, Jolanta; Sicho, Petr; Zabinski, Bartlomiej

    The ATLAS Forward Proton (AFP) detector is one of the forward detectors of the ATLAS experiment at CERN aiming at measuring momenta and angles of diffractively scattered protons. Silicon Tracking and Time-of-Flight detectors are located inside Roman Pot stations inserted into beam pipe aperture. The AFP detector is composed of two stations on each side of the ATLAS interaction point and is under commissioning. The detector is provided with high and low voltage distribution systems. Each station has vacuum and cooling systems, movement control and all the required electronics for signal processing. Monitoring of environmental parameters, like temperature and radiation, is also available. The Detector Control System (DCS) provides control and monitoring of the detector hardware and ensures the safe and reliable operation of the detector, assuring good data quality. Comparing with DCS systems of other detectors, the AFP DCS main challenge is to cope with the large variety of AFP equipment. This paper describes t...

  6. LEDA LLRF control system characterization

    International Nuclear Information System (INIS)

    Regan, A.H.; Balleyguier, P.; Ziomek, C.D.

    1998-01-01

    The Low Energy Demonstration Accelerator (LEDA) for the Accelerator for the Production of Tritium (APT) project will be built at Los Alamos National Laboratory. The low-level RF (LLRF) control system portion of this accelerator must perform many functions, of which the primary one is controlling the RF fields in the accelerating cavities. Plans have been made to provide for on-line characterization of the LLRF control system and the complete RF system through use of stimulus and response buffers, and a digital signal processor built into the field control system electronics. The purpose of this circuitry is to characterize the behavior of the entire RF system (klystron, waveguides, high power splitters, accelerator cavity, etc.). This characterization feature can be used to measure the performance of the closed loop system with respect to the open loop system, to provide an automated way to set loop parameters, to determine the cavity Q-curve, and to detect any abnormal behavior in the RF chain. The types of measurements include frequency and time-domain responses to given perturbations, amplitude modulations, etc. This paper will discuss types of algorithms that can be implemented and present a description and block diagram of the electronics to be used

  7. The new Aladdin control system

    International Nuclear Information System (INIS)

    Stott, J.P.; Eisert, D.E.

    1990-01-01

    The preliminary design of the new control system for the Aladdin electron-storage-ring light source was described at the Los Alamos workshop and an updated report was given at the Villars conference. The new system is now almost completely installed and this paper is a final report on the project. The major features of the system are the following: commercial hardware is used throughout the computer, network and microcomputer systems; the operator consoles and the device-control microcomputers are linked by Ethernet; VAXstations with keyboard and track-ball input devices are used in the operator consoles; the microcomputers use 68000-based single-board computers and VMEbus backplanes; the main magnet power supplies contain intelligent controllers which are linked to a microcomputer by a multidrop serial link; the microcomputer software is developed on the VAXstations; it is downloaded into RAM and debugged over the Ethernet before being burned into EPROMs for operational use. This paper describes the design of the system, highlighting changes from the previous proposals, and discusses the aspects which allowed the control system of an operating facility to be rebuilt without requiring or causing any significant downtime. The performance of the new system and possible future improvements are also discussed. (orig.)

  8. Development of similarity theory for control systems

    Science.gov (United States)

    Myshlyaev, L. P.; Evtushenko, V. F.; Ivushkin, K. A.; Makarov, G. V.

    2018-05-01

    The area of effective application of the traditional similarity theory and the need necessity of its development for systems are discussed. The main statements underlying the similarity theory of control systems are given. The conditions for the similarity of control systems and the need for similarity control control are formulated. Methods and algorithms for estimating and similarity control of control systems and the results of research of control systems based on their similarity are presented. The similarity control of systems includes the current evaluation of the degree of similarity of control systems and the development of actions controlling similarity, and the corresponding targeted change in the state of any element of control systems.

  9. Component Control System for a Vehicle

    Science.gov (United States)

    Fraser-Chanpong, Nathan (Inventor); Spain, Ivan (Inventor); Dawson, Andrew D. (Inventor); Bluethmann, William J. (Inventor); Lee, Chunhao J. (Inventor); Vitale, Robert L. (Inventor); Guo, Raymond (Inventor); Waligora, Thomas M. (Inventor); Akinyode, Akinjide Akinniyi (Inventor); Reed, Ryan M. (Inventor)

    2016-01-01

    A vehicle includes a chassis, a modular component, and a central operating system. The modular component is supported by the chassis. The central operating system includes a component control system, a primary master controller, and a secondary master controller. The component control system is configured for controlling the modular component. The primary and secondary master controllers are in operative communication with the component control system. The primary and secondary master controllers are configured to simultaneously transmit commands to the component control system. The component control system is configured to accept commands from the secondary master controller only when a fault occurs in the primary master controller.

  10. Digital control system of advanced reactor

    International Nuclear Information System (INIS)

    Peng Huaqing; Zhang Rui; Liu Lixin

    2001-01-01

    This article produced the Digital Control System For Advanced Reactor made by NPIC. This system uses Siemens SIMATIC PCS 7 process control system and includes five control system: reactor power control system, pressurizer level control system, pressurizer pressure control system, steam generator water level control system and dump control system. This system uses three automatic station to realize the function of five control system. Because the safety requisition of reactor is very strict, the system is redundant. The system configuration uses CFC and SCL. the human-machine interface is configured by Wincc. Finally the system passed the test of simulation by using RETRAN 02 to simulate the control object. The research solved the key technology of digital control system of reactor and will be very helpful for the nationalization of digital reactor control system

  11. Optimal source coding, removable noise elimination, and natural coordinate system construction for general vector sources using replicator neural networks

    Science.gov (United States)

    Hecht-Nielsen, Robert

    1997-04-01

    A new universal one-chart smooth manifold model for vector information sources is introduced. Natural coordinates (a particular type of chart) for such data manifolds are then defined. Uniformly quantized natural coordinates form an optimal vector quantization code for a general vector source. Replicator neural networks (a specialized type of multilayer perceptron with three hidden layers) are the introduced. As properly configured examples of replicator networks approach minimum mean squared error (e.g., via training and architecture adjustment using randomly chosen vectors from the source), these networks automatically develop a mapping which, in the limit, produces natural coordinates for arbitrary source vectors. The new concept of removable noise (a noise model applicable to a wide variety of real-world noise processes) is then discussed. Replicator neural networks, when configured to approach minimum mean squared reconstruction error (e.g., via training and architecture adjustment on randomly chosen examples from a vector source, each with randomly chosen additive removable noise contamination), in the limit eliminate removable noise and produce natural coordinates for the data vector portions of the noise-corrupted source vectors. Consideration regarding selection of the dimension of a data manifold source model and the training/configuration of replicator neural networks are discussed.

  12. Increasing the Number of Replications in Item Response Theory Simulations: Automation through SAS and Disk Operating System

    Science.gov (United States)

    Gagne, Phill; Furlow, Carolyn; Ross, Terris

    2009-01-01

    In item response theory (IRT) simulation research, it is often necessary to use one software package for data generation and a second software package to conduct the IRT analysis. Because this can substantially slow down the simulation process, it is sometimes offered as a justification for using very few replications. This article provides…

  13. Influence of process parameters on edge replication quality of lab-on-a-chip micro fluidic systems geometries

    DEFF Research Database (Denmark)

    Calaon, Matteo; Tosello, Guido; Hansen, Hans Nørgaard

    2013-01-01

    the replication quality of molded micro-features on cyclic olefin copolymer (COC) a tool insert collecting critical channel cross sections was manufactured. The master was made by UV lithography and subsequent nickel electroplating. Effect of packing phase parameters (packing time, packing pressure) and mold...

  14. The CESR computer control system

    International Nuclear Information System (INIS)

    Helmke, R.G.; Rice, D.H.; Strohman, C.

    1986-01-01

    The control system for the Cornell Electron Storage Ring (CESR) has functioned satisfactorily since its implementation in 1979. Key characteristics are fast tuning response, almost exclusive use of FORTRAN as a programming language, and efficient coordinated ramping of CESR guide field elements. This original system has not, however, been able to keep pace with the increasing complexity of operation of CESR associated with performance upgrades. Limitations in address space, expandability, access to data system-wide, and program development impediments have prompted the undertaking of a major upgrade. The system under development accomodates up to 8 VAX computers for all applications programs. The database and communications semaphores reside in a shared multi-ported memory, and each hardware interface bus is controlled by a dedicated 32 bit micro-processor in a VME based system. (orig.)

  15. Rational Synthesis of Hollow Prussian Blue Analogue Through Coordination Replication and Controlled-Etching for Cs-Ion Removal.

    Science.gov (United States)

    Xu, Jun; Bu, Fan-Xing; Guo, Yi-Fei; Zhang, Wei; Hu, Ming; Jiang, Ji-Sen

    2018-05-01

    Radioactive cesium pollution have received considerable attention due to the increasing risks in development of the nuclear power plants in the world. Although various functional porous materials are utilized to adsorb Cs+ ions in water, Prussian blue analogues (PBAs) are an impressive class of candidates because of their super affinity of Cs+ ions. The adsorption ability of the PBAs strongly relate to the mesostructure and interstitial sites. To design a hollow PBA with large number of interstitial sites, the traditional hollowing methods are not suitable owing to the difficulty in processing the specific PBAs with large number of interstitial sites. In this work, we empolyed a rational strategy which was to form a "metal oxide"@"PBA" core-shell structure via coordination replication at first, then utilized a mild etching to remove the metal oxide core, led to hollow PBA finally. The obtained hollow PBAs were of high crystallinity and large number of interstitial sites, showing a super adsorption performance for Cs+ ions (221.6 mg/g) within a short period (10 min).

  16. Assessment of heterogeneity between European Populations: a Baltic and Danish replication case-control study of SNPs from a recent European ulcerative colitis genome wide association study.

    Science.gov (United States)

    Andersen, Vibeke; Ernst, Anja; Sventoraityte, Jurgita; Kupcinskas, Limas; Jacobsen, Bent A; Krarup, Henrik B; Vogel, Ulla; Jonaitis, Laimas; Denapiene, Goda; Kiudelis, Gediminas; Balschun, Tobias; Franke, Andre

    2011-10-13

    Differences in the genetic architecture of inflammatory bowel disease between different European countries and ethnicities have previously been reported. In the present study, we wanted to assess the role of 11 newly identified UC risk variants, derived from a recent European UC genome wide association study (GWAS) (Franke et al., 2010), for 1) association with UC in the Nordic countries, 2) for population heterogeneity between the Nordic countries and the rest of Europe, and, 3) eventually, to drive some of the previous findings towards overall genome-wide significance. Eleven SNPs were replicated in a Danish sample consisting of 560 UC patients and 796 controls and nine missing SNPs of the German GWAS study were successfully genotyped in the Baltic sample comprising 441 UC cases and 1156 controls. The independent replication data was then jointly analysed with the original data and systematic comparisons of the findings between ethnicities were made. Pearson's χ2, Breslow-Day (BD) and Cochran-Mantel-Haenszel (CMH) tests were used for association analyses and heterogeneity testing. The rs5771069 (IL17REL) SNP was not associated with UC in the Danish panel. The rs5771069 (IL17REL) SNP was significantly associated with UC in the combined Baltic, Danish and Norwegian UC study sample driven by the Norwegian panel (OR = 0.89, 95% CI: 0.79-0.98, P = 0.02). No association was found between rs7809799 (SMURF1/KPNA7) and UC (OR = 1.20, 95% CI: 0.95-1.52, P = 0.10) or between UC and all other remaining SNPs. We had 94% chance of detecting an association for rs7809799 (SMURF1/KPNA7) in the combined replication sample, whereas the power were 55% or lower for the remaining SNPs.Statistically significant PBD was found for OR heterogeneity between the combined Baltic, Danish, and Norwegian panel versus the combined German, British, Belgian, and Greek panel (rs7520292 (P = 0.001), rs12518307 (P = 0.007), and rs2395609 (TCP11) (P = 0.01), respectively).No SNP reached genome

  17. Assessment of heterogeneity between European Populations: a Baltic and Danish replication case-control study of SNPs from a recent European ulcerative colitis genome wide association study

    Directory of Open Access Journals (Sweden)

    Jonaitis Laimas

    2011-10-01

    Full Text Available Abstract Background Differences in the genetic architecture of inflammatory bowel disease between different European countries and ethnicities have previously been reported. In the present study, we wanted to assess the role of 11 newly identified UC risk variants, derived from a recent European UC genome wide association study (GWAS (Franke et al., 2010, for 1 association with UC in the Nordic countries, 2 for population heterogeneity between the Nordic countries and the rest of Europe, and, 3 eventually, to drive some of the previous findings towards overall genome-wide significance. Methods Eleven SNPs were replicated in a Danish sample consisting of 560 UC patients and 796 controls and nine missing SNPs of the German GWAS study were successfully genotyped in the Baltic sample comprising 441 UC cases and 1156 controls. The independent replication data was then jointly analysed with the original data and systematic comparisons of the findings between ethnicities were made. Pearson's χ2, Breslow-Day (BD and Cochran-Mantel-Haenszel (CMH tests were used for association analyses and heterogeneity testing. Results The rs5771069 (IL17REL SNP was not associated with UC in the Danish panel. The rs5771069 (IL17REL SNP was significantly associated with UC in the combined Baltic, Danish and Norwegian UC study sample driven by the Norwegian panel (OR = 0.89, 95% CI: 0.79-0.98, P = 0.02. No association was found between rs7809799 (SMURF1/KPNA7 and UC (OR = 1.20, 95% CI: 0.95-1.52, P = 0.10 or between UC and all other remaining SNPs. We had 94% chance of detecting an association for rs7809799 (SMURF1/KPNA7 in the combined replication sample, whereas the power were 55% or lower for the remaining SNPs. Statistically significant PBD was found for OR heterogeneity between the combined Baltic, Danish, and Norwegian panel versus the combined German, British, Belgian, and Greek panel (rs7520292 (P = 0.001, rs12518307 (P = 0.007, and rs2395609 (TCP11 (P = 0

  18. Proton beam therapy control system

    Science.gov (United States)

    Baumann, Michael A [Riverside, CA; Beloussov, Alexandre V [Bernardino, CA; Bakir, Julide [Alta Loma, CA; Armon, Deganit [Redlands, CA; Olsen, Howard B [Colton, CA; Salem, Dana [Riverside, CA

    2008-07-08

    A tiered communications architecture for managing network traffic in a distributed system. Communication between client or control computers and a plurality of hardware devices is administered by agent and monitor devices whose activities are coordinated to reduce the number of open channels or sockets. The communications architecture also improves the transparency and scalability of the distributed system by reducing network mapping dependence. The architecture is desirably implemented in a proton beam therapy system to provide flexible security policies which improve patent safety and facilitate system maintenance and development.

  19. Control synthesis of switched systems

    CERN Document Server

    Zhao, Xudong; Niu, Ben; Wu, Tingting

    2017-01-01

    This book offers its readers a detailed overview of the synthesis of switched systems, with a focus on switching stabilization and intelligent control. The problems investigated are not only previously unsolved theoretically but also of practical importance in many applications: voltage conversion, naval piloting and navigation and robotics, for example. The book considers general switched-system models and provides more efficient design methods to bring together theory and application more closely than was possible using classical methods. It also discusses several different classes of switched systems. For general switched linear systems and switched nonlinear systems comprising unstable subsystems, it introduces novel ideas such as invariant subspace theory and the time-scheduled Lyapunov function method of designing switching signals to stabilize the underlying systems. For some typical switched nonlinear systems affected by various complex dynamics, the book proposes novel design approaches based on inte...

  20. Photoirradiation system with temperature control

    International Nuclear Information System (INIS)

    Yonadab Lopez, F.; Stolik, S.; La Rosa, J. M. de; Moreno, E.

    2012-01-01

    During application of phototherapy is possible to induce a significant increase in tissue temperature and generate a localized hyperthermia state if the power density of incident light is high enough. We present a controlled temperature phototherapy system, this allows the application of optical radiation at a wavelength of 630nm using a light emitting diode (LED) of high power. The system automatically controls the irradiation time and power which allows irradiating the tissue with an appropriate energy density. A thermocouple is placed in the irradiated tissue to measure and control the temperature by varying the parameters of power density and time. From results of irradiations made in nu / nu mice using doses of 150 J/cm 2 energy and 250 J/cm 2 shows that the temperature control allows the study of photodynamic therapy in synergy with thermo therapy in different diseases external tissues. (Author)

  1. Trends in accelerator control systems

    International Nuclear Information System (INIS)

    Crowley-Milling, M.C.

    1984-04-01

    Over the years, we have seen a revolution in control systems that has followed the ever decreasing cost of computer power and memory. It started with the data gathering, when people distrusted the computer to perform control actions correctly, through the stage of using a computer to perform control actions correctly, through the stage of using a computer system to provide a convenient remote look and adjust facility, to the present day, when more and more emphasis is being placed on using a computer system to simulate or model all or parts of the accelerator, feed in the required performance and calling for the computers to set the various parameters and then measure the actual performance, with iteration if necessary. The progress that has been made in the fields of architecture, communications, computers, interface, software design and operator interface is reviewed

  2. Radiation-hardened control system

    International Nuclear Information System (INIS)

    Vandermolen, R.I.; Smith, S.F.; Emery, M.S.

    1993-01-01

    A radiation-hardened bit-slice control system with associated input/output circuits was developed to prove that programmable circuits could be constructed to successfully implement intelligent functions in a highly radioactive environment. The goal for this effort was to design and test a programmable control system that could withstand a minimum total dose of 10 7 rads (gamma). The Radiation Hardened Control System (RHCS) was tested in operation at a dose rate that ranged up to 135 krad/h, with an average total dose of 10.75 Mrads. Further testing beyond the required 10 7 rads was also conducted. RHCS performed properly through the target dose of 10 7 rads, and sporadic intermittent failures in some programmable logic devices were noted after ∼ 13 Mrads

  3. Power system dynamics and control

    CERN Document Server

    Kwatny, Harry G

    2016-01-01

    This monograph explores a consistent modeling and analytic framework that provides the tools for an improved understanding of the behavior and the building of efficient models of power systems. It covers the essential concepts for the study of static and dynamic network stability, reviews the structure and design of basic voltage and load-frequency regulators, and offers an introduction to power system optimal control with reliability constraints. A set of Mathematica tutorial notebooks providing detailed solutions of the examples worked-out in the text, as well as a package that will enable readers to work out their own examples and problems, supplements the text. A key premise of the book is that the design of successful control systems requires a deep understanding of the processes to be controlled; as such, the technical discussion begins with a concise review of the physical foundations of electricity and magnetism. This is followed by an overview of nonlinear circuits that include resistors, inductors, ...

  4. Advanced Light Source control system

    International Nuclear Information System (INIS)

    Magyary, S.; Chin, M.; Cork, C.; Fahmie, M.; Lancaster, H.; Molinari, P.; Ritchie, A.; Robb, A.; Timossi, C.

    1989-03-01

    The Advanced Light Source (ALS) is a third generation 1--2 GeV synchrotron radiation source designed to provide ports for 60 beamlines. It uses a 50 MeV electron linac and 1.5 GeV, 1 Hz, booster synchrotron for injection into a 1--2 GeV storage ring. Interesting control problems are created because of the need for dynamic closed beam orbit control to eliminate interaction between the ring tuning requirements and to minimize orbit shifts due to ground vibrations. The extremely signal sensitive nature of the experiments requires special attention to the sources of electrical noise. These requirements have led to a control system design which emphasizes connectivity at the accelerator equipment end and a large I/O bandwidth for closed loop system response. Not overlooked are user friendliness, operator response time, modeling, and expert system provisions. Portable consoles are used for local operation of machine equipment. Our solution is a massively parallel system with >120 Mbits/sec I/O bandwidth and >1500 Mips computing power. At the equipment level connections are made using over 600 powerful Intelligent Local Controllers (ILC-s) mounted in 3U size Eurocard slots using fiber-optic cables between rack locations. In the control room, personal computers control and display all machine variables at a 10 Hz rate including the scope signals which are collected though the control system. Commercially available software and industry standards are used extensively. Particular attention is paid to reliability, maintainability and upgradeability. 10 refs., 11 figs

  5. Regulation on control systems tests

    International Nuclear Information System (INIS)

    Grau, J.; Navarro, J.M.

    1978-01-01

    Requirements under regulation applicable to the testing of control systems and controlled equipments in the case of USA nuclear projects are examined. They are reviewed, in particular, the following standards and criteria: 10 Code of Federal Regulations 50, Appendix A, General Design Criteria 20 and 21; IEEE Standards 279 and 308; IEEE Standard 338; US Regulatory Guides 1.22 and 1.118.(J.E.de C.)

  6. Control systems for experimental physics

    International Nuclear Information System (INIS)

    Anon.

    1988-01-01

    At an international conference last year at Villars-sur-Ollon (Switzerland), scientists from all over the world looked at the problems of controlling complex physics installations, including particle accelerators, nuclear reactors, large telescopes and high energy physics detectors. The meeting, organized by the European Physical Society's Interdivisional Group on Experimental Physics Control Systems, EPCS, brought together 180 scientists from the world's leading experimental physics research laboratories, universities and industries

  7. Control of HIV replication in astrocytes by a family of highly conserved host proteins with a common Rev-interacting domain (Risp).

    Science.gov (United States)

    Vincendeau, Michelle; Kramer, Susanne; Hadian, Kamyar; Rothenaigner, Ina; Bell, Jeanne; Hauck, Stefanie M; Bickel, Christian; Nagel, Daniel; Kremmer, Elisabeth; Werner, Thomas; Leib-Mösch, Christine; Brack-Werner, Ruth

    2010-10-23

    In human astrocytes, restriction of HIV replication involves inhibition of HIV Rev activity. We previously identified a Rev-interacting human protein fragment (16.4.1) that can reduce Rev activity. The 16.4.1 sequence is contained in a group of highly similar host cell proteins, which we call the Risp family. Here we investigate whether the Risp family is connected to HIV replication in astrocytes. Cell/tissue lysates were analyzed for Risp expression by western blot with various anti-Risp antibodies. The interaction of astrocytic Risp members with Rev was investigated by affinity chromatography. Astrocytes were transfected with expression plasmids containing cDNAs encoding full-length Risp or the isolated 16.4.1 region for Risp overexpression or with siRNAs designed for Risp knock-down. Rev activity was investigated with a Rev-reporter assay. RNA levels were quantified by real-time RT-PCR, HIV Gag levels by p24ELISA. Expression of the Risp family was demonstrated in human brain tissues and astrocytes. Astrocytes were shown to produce Risp family members that interact with Rev. Production of HIV Gag proteins and Rev-dependent RNAs in persistently infected astrocytes increased upon Risp knock-down and decreased upon Risp overexpression. Risp knock-down increased Rev activity and raised proportions of Rev proteins in the nucleus of astrocytes. Our results link the Risp family to restriction of HIV production and inhibition of Rev activity in astrocytes. We conclude that the Risp family represents a novel family of host factors that can control HIV replication and may be important for the containment of HIV infection in brain reservoirs.

  8. The CMS tracker control system

    International Nuclear Information System (INIS)

    Dierlamm, A; Dirkes, G H; Fahrer, M; Frey, M; Hartmann, F; Masetti, L; Militaru, O; Shah, S Y; Stringer, R; Tsirou, A

    2008-01-01

    The Tracker Control System (TCS) is a distributed control software to operate about 2000 power supplies for the silicon modules of the CMS Tracker and monitor its environmental sensors. TCS must thus be able to handle about 10 4 power supply parameters, about 10 3 environmental probes from the Programmable Logic Controllers of the Tracker Safety System (TSS), about 10 5 parameters read via DAQ from the DCUs in all front end hybrids and from CCUs in all control groups. TCS is built on top of an industrial SCADA program (PVSS) extended with a framework developed at CERN (JCOP) and used by all LHC experiments. The logical partitioning of the detector is reflected in the hierarchical structure of the TCS, where commands move down to the individual hardware devices, while states are reported up to the root which is interfaced to the broader CMS control system. The system computes and continuously monitors the mean and maximum values of critical parameters and updates the percentage of currently operating hardware. Automatic procedures switch off selected parts of the detector using detailed granularity and avoiding widespread TSS intervention

  9. The CMS tracker control system

    Science.gov (United States)

    Dierlamm, A.; Dirkes, G. H.; Fahrer, M.; Frey, M.; Hartmann, F.; Masetti, L.; Militaru, O.; Shah, S. Y.; Stringer, R.; Tsirou, A.

    2008-07-01

    The Tracker Control System (TCS) is a distributed control software to operate about 2000 power supplies for the silicon modules of the CMS Tracker and monitor its environmental sensors. TCS must thus be able to handle about 104 power supply parameters, about 103 environmental probes from the Programmable Logic Controllers of the Tracker Safety System (TSS), about 105 parameters read via DAQ from the DCUs in all front end hybrids and from CCUs in all control groups. TCS is built on top of an industrial SCADA program (PVSS) extended with a framework developed at CERN (JCOP) and used by all LHC experiments. The logical partitioning of the detector is reflected in the hierarchical structure of the TCS, where commands move down to the individual hardware devices, while states are reported up to the root which is interfaced to the broader CMS control system. The system computes and continuously monitors the mean and maximum values of critical parameters and updates the percentage of currently operating hardware. Automatic procedures switch off selected parts of the detector using detailed granularity and avoiding widespread TSS intervention.

  10. Integrated Control System Engineering Support.

    Science.gov (United States)

    1984-12-01

    Advanced Medium Range Air to Air Missile ASTEC Advanced Speech Technology Experimental Configuration BA Body Axis BCIU Bus Control Interface Unit BMU Bus...support nreeded to tie an ASTEC speech recognition system into the DIGISYN fJcility and support an FIGR experiment designed to investigate the voice...information passed to the PDP computer consisted of integers which represented words or phrases recognized by the ASTEC recognition system. An interface

  11. Satellite Attitude Control System Simulator

    Directory of Open Access Journals (Sweden)

    G.T. Conti

    2008-01-01

    Full Text Available Future space missions will involve satellites with great autonomy and stringent pointing precision, requiring of the Attitude Control Systems (ACS with better performance than before, which is function of the control algorithms implemented on board computers. The difficulties for developing experimental ACS test is to obtain zero gravity and torque free conditions similar to the SCA operate in space. However, prototypes for control algorithms experimental verification are fundamental for space mission success. This paper presents the parameters estimation such as inertia matrix and position of mass centre of a Satellite Attitude Control System Simulator (SACSS, using algorithms based on least square regression and least square recursive methods. Simulations have shown that both methods have estimated the system parameters with small error. However, the least square recursive methods have performance more adequate for the SACSS objectives. The SACSS platform model will be used to do experimental verification of fundamental aspects of the satellite attitude dynamics and design of different attitude control algorithm.

  12. Codon and amino acid usage in two major human pathogens of genus Bartonella--optimization between replicational-transcriptional selection, translational control and cost minimization.

    Science.gov (United States)

    Das, Sabyasachi; Paul, Sandip; Chatterjee, Sanjib; Dutta, Chitra

    2005-01-01

    Intra-genomic variation in synonymous codon and amino acid usage in two human pathogens Bartonella henselae and B. quintana has been carried out through multivariate analysis. Asymmetric mutational bias, coupled with replicational-transcriptional selection, has been identified as the prime selection force behind synonymous codon selection--a characteristic of the genus Bartonella, not exhibited by any other alpha-proteobacterial genome. Distinct codon usage patterns and low synonymous divergence values between orthologous sequences of highly expressed genes from the two Bartonella species indicate that there exists a residual intra-strand synonymous codon bias in the highly expressed genes, possibly operating at the level of translation. In the case of amino acid usage, the mean hydropathy level and aromaticity are the major sources of variation, both having nearly equal impact, while strand-specific mutational pressure and gene expressivity strongly influence the inter-strand variations. In both species under study, the highly expressed gene products tend not to contain heavy and/or aromatic residues, following the cost-minimization hypothesis in spite of their intracellular lifestyle. The codon and amino acid usage in these two human pathogens are, therefore, consequences of a complex balance between replicational-transcriptional selection, translational control, protein hydropathy and cost minimization.

  13. Cockpit control system conceptual design

    Science.gov (United States)

    Meholic, Greg; Brown, Rhonda; Hall, Melissa; Harvey, Robert; Singer, Michael; Tella, Gustavo

    1993-01-01

    The purpose of this project was to provide a means for operating the ailerons, elevator, elevator trim, rudder, nosewheel steering, and brakes in the Triton primary flight trainer. The main design goals under consideration were to illustrate system and subsystem integration, control function ability, and producibility. Weight and maintenance goals were addressed.

  14. Neutron flux control systems validation

    International Nuclear Information System (INIS)

    Hascik, R.

    2003-01-01

    In nuclear installations main requirement is to obtain corresponding nuclear safety in all operation conditions. From the nuclear safety point of view is commissioning and start-up after reactor refuelling appropriate period for safety systems verification. In this paper, methodology, performance and results of neutron flux measurements systems validation is presented. Standard neutron flux measuring chains incorporated into the reactor protection and control system are used. Standard neutron flux measuring chain contains detector, preamplifier, wiring to data acquisition unit, data acquisition unit, wiring to display at control room and display at control room. During reactor outage only data acquisition unit and wiring and displaying at reactor control room is verified. It is impossible to verify detector, preamplifier and wiring to data acquisition recording unit during reactor refuelling according to low power. Adjustment and accurate functionality of these chains is confirmed by start-up rate (SUR) measurement during start-up tests after refuelling of the reactors. This measurement has direct impact to nuclear safety and increase operational nuclear safety level. Briefly description of each measuring system is given. Results are illustrated on measurements performed at Bohunice NPP during reactor start-up tests. Main failures and their elimination are described (Authors)

  15. Controlling dynamics in diatomic systems

    Indian Academy of Sciences (India)

    WINTEC

    Abstract. Controlling molecular energetics using laser pulses is exemplified for nuclear motion in two different diatomic systems. The problem of finding the optimized field for maximizing a desired quantum dynamical target is formulated using an iterative method. The method is applied for two diatomic sys- tems, HF and OH.

  16. Beam based systems and controls

    CERN Document Server

    Jacquet, D

    2012-01-01

    This presentation will give a review from the operations team of the performance and issues of the beam based systems, namely RF, ADT, beam instrumentation, controls and injection systems. For each of these systems, statistics on performance and availability will be presented with the main issues encountered in 2012. The possible improvements for operational efficiency and safety will be discussed, with an attempt to answer the question "Are we ready for the new challenges brought by the 25ns beam and increased energy after LSI? ".

  17. Accelerator control systems without minicomputers

    International Nuclear Information System (INIS)

    Altaber, J.; Beck, F.; Rausch, R.

    1980-01-01

    A paper given last year described in general terms a plan for the control of a large machine using assemblies of microcomputer units which simulate a conventional minicomputer by multiprocessing. In every other way the SPS control philosophy is followed. The design of a model assembly has allowed us to learn something about the protocols needed inside and between assemblies, as well as to assess more accurately what level of technology it is reasonable to apply. In any control system of this kind it would be desirable to allow engineering contributions from a variety of sources, and yet ensure the homogeneity needed for the system to remain reliable and comprehensible. Methods of achieving this are discussed. (Auth.)

  18. The ATLAS detector control system

    International Nuclear Information System (INIS)

    Schlenker, S.; Arfaoui, S.; Franz, S.

    2012-01-01

    The ATLAS experiment is one of the multi-purpose experiments at the Large Hadron Collider (LHC), constructed to study elementary particle interactions in collisions of high-energy proton beams. Twelve different sub-detectors as well as the common experimental infrastructure are supervised by the Detector Control System (DCS). The DCS enables equipment supervision of all ATLAS sub-detectors by using a system of more that 130 server machines running the industrial SCADA product PVSS. This highly distributed system reads, processes and archives of the order of 10 6 operational parameters. Higher level control system layers allow for automatic control procedures, efficient error recognition and handling, and manage the communication with external systems such as the LHC. First, this contribution describes the status of the ATLAS DCS and the experience gained during the LHC commissioning and the first physics data taking operation period. Secondly, the future evolution and maintenance constraints for the coming years and the LHC high luminosity upgrades are outlined. (authors)

  19. Lighting system with illuminance control

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention relates to an illumination control system comprising a plurality of outdoor luminaries and a motorized service vehicle. Each luminaire comprises a controllable light source producing a light illuminance. The motorized service vehicle comprises a light sensor configured...... to detect the light illuminance generated by the controllable light source at the motorized service vehicle. The motorized service vehicle computes light illuminance data based on the detected light illuminance and transmits these to the outdoor luminaire through a wireless communication link or stores...... the light illuminance data on a data recording device of the motorized service vehicle. The outdoor luminaire receives may use the light illuminance data to set or adjust a light illuminance of the controllable light source....

  20. Robust lyapunov controller for uncertain systems

    KAUST Repository

    Laleg-Kirati, Taous-Meriem; Elmetennani, Shahrazed

    2017-01-01

    Various examples of systems and methods are provided for Lyapunov control for uncertain systems. In one example, a system includes a process plant and a robust Lyapunov controller configured to control an input of the process plant. The robust

  1. The NSTX Central Instrumentation and Control System

    International Nuclear Information System (INIS)

    G. Oliaro; J. Dong; K. Tindall; P. Sichta

    1999-01-01

    Earlier this year the National Spherical Torus Experiment (NSTX) at the Princeton Plasma Physics Laboratory achieved ''first plasma''. The Central Instrumentation and Control System was used to support plasma operations. Major elements of the system include the Process Control System, Plasma Control System, Network System, Data Acquisition System, and Synchronization System. This paper will focus on the Process Control System. Topics include the architecture, hardware interface, operator interface, data management, and system performance

  2. Rapid transient production in plants by replicating and non-replicating vectors yields high quality functional anti-HIV antibody.

    Directory of Open Access Journals (Sweden)

    Frank Sainsbury

    2010-11-01

    Full Text Available The capacity of plants and plant cells to produce large amounts of recombinant protein has been well established. Due to advantages in terms of speed and yield, attention has recently turned towards the use of transient expression systems, including viral vectors, to produce proteins of pharmaceutical interest in plants. However, the effects of such high level expression from viral vectors and concomitant effects on host cells may affect the quality of the recombinant product.To assess the quality of antibodies transiently expressed to high levels in plants, we have expressed and characterised the human anti-HIV monoclonal antibody, 2G12, using both replicating and non-replicating systems based on deleted versions of Cowpea mosaic virus (CPMV RNA-2. The highest yield (approximately 100 mg/kg wet weight leaf tissue of affinity purified 2G12 was obtained when the non-replicating CPMV-HT system was used and the antibody was retained in the endoplasmic reticulum (ER. Glycan analysis by mass-spectrometry showed that the glycosylation pattern was determined exclusively by whether the antibody was retained in the ER and did not depend on whether a replicating or non-replicating system was used. Characterisation of the binding and neutralisation properties of all the purified 2G12 variants from plants showed that these were generally similar to those of the Chinese hamster ovary (CHO cell-produced 2G12.Overall, the results demonstrate that replicating and non-replicating CPMV-based vectors are able to direct the production of a recombinant IgG similar in activity to the CHO-produced control. Thus, a complex recombinant protein was produced with no apparent effect on its biochemical properties using either high-level expression or viral replication. The speed with which a recombinant pharmaceutical with excellent biochemical characteristics can be produced transiently in plants makes CPMV-based expression vectors an attractive option for

  3. Cyber Incidents Involving Control Systems

    Energy Technology Data Exchange (ETDEWEB)

    Robert J. Turk

    2005-10-01

    The Analysis Function of the US-CERT Control Systems Security Center (CSSC) at the Idaho National Laboratory (INL) has prepared this report to document cyber security incidents for use by the CSSC. The description and analysis of incidents reported herein support three CSSC tasks: establishing a business case; increasing security awareness and private and corporate participation related to enhanced cyber security of control systems; and providing informational material to support model development and prioritize activities for CSSC. The stated mission of CSSC is to reduce vulnerability of critical infrastructure to cyber attack on control systems. As stated in the Incident Management Tool Requirements (August 2005) ''Vulnerability reduction is promoted by risk analysis that tracks actual risk, emphasizes high risk, determines risk reduction as a function of countermeasures, tracks increase of risk due to external influence, and measures success of the vulnerability reduction program''. Process control and Supervisory Control and Data Acquisition (SCADA) systems, with their reliance on proprietary networks and hardware, have long been considered immune to the network attacks that have wreaked so much havoc on corporate information systems. New research indicates this confidence is misplaced--the move to open standards such as Ethernet, Transmission Control Protocol/Internet Protocol, and Web technologies is allowing hackers to take advantage of the control industry's unawareness. Much of the available information about cyber incidents represents a characterization as opposed to an analysis of events. The lack of good analyses reflects an overall weakness in reporting requirements as well as the fact that to date there have been very few serious cyber attacks on control systems. Most companies prefer not to share cyber attack incident data because of potential financial repercussions. Uniform reporting requirements will do much to make this

  4. Encyclopedia of systems and control

    CERN Document Server

    Samad, Tariq

    2015-01-01

    The Encyclopedia of Systems and Control collects a broad range of short expository articles that describe the current state of the art in the central topics of control and systems engineering as well as in many of the related fields in which control is an enabling technology. The editors have assembled the most comprehensive reference possible, and this has been greatly facilitated by the publisher’s commitment continuously to publish updates to the articles as they become available in the future. Although control engineering is now a mature discipline, it remains an area in which there is a great deal of research activity, and as new developments in both theory and applications become available, they will be included in the online version of the encyclopedia. A carefully chosen team of leading authorities in the field has written the well over 200 articles that comprise the work. The topics range from basic principles of feedback in servomechanisms to advanced topics such as the control of Boolean networks...

  5. Robust lyapunov controller for uncertain systems

    KAUST Repository

    Laleg-Kirati, Taous-Meriem

    2017-02-23

    Various examples of systems and methods are provided for Lyapunov control for uncertain systems. In one example, a system includes a process plant and a robust Lyapunov controller configured to control an input of the process plant. The robust Lyapunov controller includes an inner closed loop Lyapunov controller and an outer closed loop error stabilizer. In another example, a method includes monitoring a system output of a process plant; generating an estimated system control input based upon a defined output reference; generating a system control input using the estimated system control input and a compensation term; and adjusting the process plant based upon the system control input to force the system output to track the defined output reference. An inner closed loop Lyapunov controller can generate the estimated system control input and an outer closed loop error stabilizer can generate the system control input.

  6. Control system for gamma camera

    International Nuclear Information System (INIS)

    Miller, D.W.

    1977-01-01

    An improved gamma camera arrangement is described which utilizing a solid state detector, formed of high purity germanium. the central arrangement of the camera operates to effect the carrying out of a trapezoidal filtering operation over antisymmetrically summed spatial signals through gated integration procedures utilizing idealized integrating intervals. By simultaneously carrying out peak energy evaluation of the input signals, a desirable control over pulse pile-up phenomena is achieved. Additionally, through the use of the time derivative of incoming pulse or signal energy information to initially enable the control system, a low level information evaluation is provided serving to enhance the signal processing efficiency of the camera

  7. Isolation contactor state control system

    Energy Technology Data Exchange (ETDEWEB)

    Bissontz, Jay E.

    2017-05-16

    A controller area network (CAN) installed on a hybrid electric vehicle provides one node with control of high voltage power distribution system isolation contactors and the capacity to energize a secondary electro-mechanical relay device. The output of the secondary relay provides a redundant and persistent backup signal to the output of the node. The secondary relay is relatively immune to CAN message traffic interruptions and, as a result, the high voltage isolation contactor(s) are less likely to transition open in the event that the intelligent output driver should fail.

  8. Quantum control of optomechanical systems

    International Nuclear Information System (INIS)

    Hofer, S.

    2015-01-01

    This thesis explores the prospects of entanglement-enhanced quantum control of optomechanical systems. We first discuss several pulsed schemes in which the radiation-pressure interaction is used to generate EPR entanglement between the mechanical mode of a cavity-optomechanical system and a travelling-wave light pulse. The entanglement created in this way can be used as a resource for mechanical state preparation. On the basis of this protocol, we introduce an optomechanical teleportation scheme to transfer an arbitrary light state onto the mechanical system. Furthermore, we describe how one can create a mechanical non-classical state (i.e., a state with a negative Wigner function) by single-photon detection, and, in a similar protocol, how optomechanical systems can be used to demonstrate the violation of a Bell inequality. The second part of the thesis is dedicated to time-continuous quantum control protocols. Making use of optimal-control techniques, we analyse measurement-based feedback cooling of a mechanical oscillator and demonstrate that ground-state cooling is achievable in the sideband-resolved, blue-detuned regime. We then extend this homodyne-detection based setup and introduce the notion of a time-continuous Bell measurement---a generalisation of the standard continuous variable Bell measurement to a continuous measurement setting. Combining this concept with continuous feedback we analyse the generation of a squeezed mechanical steady state via time-continuous teleportation, and the creation of bipartite mechanical entanglement by entanglement swapping. Finally we discuss an experiment demonstrating the evaluation of the conditional optomechanical quantum state by Kalman filtering, constituting a important step towards time-continuous quantum control of optomechanical systems and the possible realisation of the protocols presented in this thesis. (author) [de

  9. Multiagent voltage and reactive power control system

    Directory of Open Access Journals (Sweden)

    I. Arkhipov

    2014-12-01

    Full Text Available This paper is devoted to the research of multiagent voltage and reactive power control system development. The prototype of the system has been developed by R&D Center at FGC UES (Russia. The control system architecture is based on the innovative multiagent system theory application that leads to the achievement of several significant advantages (in comparison to traditional control systems implementation such as control system efficiency enhancement, control system survivability and cyber security.

  10. Replication of recently identified systemic lupus erythematosus genetic associations: a case-control study

    Czech Academy of Sciences Publication Activity Database

    Suarez-Gestal, M.; Calaza, M.; Endreffy, E.; Pullmann, R.; Ordi-Ros, J.; Sebastiani, D.G.; Růžičková, Šárka; Santos, J.M.; Papasteriades, C.; Marchini, M.; Skopouli, F.N.; Suarez, A.; Blanco, F.J.; D'Alfonso, S.; Bijl, M.; Carreira, P.; Witte, T.; Migliaresi, S.; Gomez-Reino, J.J.; Gonzalez, A.

    2009-01-01

    Roč. 11, č. 3 (2009), R69 ISSN 1478-6362 Keywords : Single nucleotide polymorphism susceptibility * sytemic lupus erythematosus Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.271, year: 2009

  11. A cluster randomized control field trial of the ABRACADABRA web-based literacy intervention: Replication and extension of basic findings.

    Directory of Open Access Journals (Sweden)

    Noella Angele Piquette

    2014-12-01

    Full Text Available The present paper reports a cluster randomized control trial evaluation of teaching using ABRACADABRA (ABRA, an evidence-based and web-based literacy intervention (http://abralite.concordia.ca with 107 kindergarten and 96 grade 1 children in 24 classes (12 intervention 12 control classes from all 12 elementary schools in one school district in Canada. Children in the intervention condition received 10-12 hours of whole class instruction using ABRA between pre- and post-test. Hierarchical linear modeling of post-test results showed significant gains in letter-sound knowledge for intervention classrooms over control classrooms. In addition, medium effect sizes were evident for three of five outcome measures favoring the intervention: letter-sound knowledge (d = +.66, phonological blending (d = +.52, and word reading (d = +.52, over effect sizes for regular teaching. It is concluded that regular teaching with ABRA technology adds significantly to literacy in the early elementary years.

  12. Plug-and-Play Control – Modifying Control Systems Online

    DEFF Research Database (Denmark)

    Bendtsen, Jan Dimon; Trangbæk, K; Stoustrup, Jakob

    2013-01-01

    Often, when new sensor or actuator hardware be- comes available for use in a control system, it is desirable to retain the existing control system and apply the new control capabilities in a gradual fashion rather than decommissioning the entire existing system and replacing it with an altogether...... new control system. However, this requires that the existing controller remains in action, and the new control law component is added to the existing system. This paper formally introduces the concept of Plug-and-Play control and proposes two different methods of introducing new control components...

  13. Regulation of human histone gene expression: transcriptional and posttranscriptional control in the coupling of histone messenger RNA stability with DNA replication

    International Nuclear Information System (INIS)

    Baumbach, L.L.; Stein, G.S.; Stein, J.L.

    1987-01-01

    The extent to which transcriptional and posttranscriptional regulation contributes to the coupling of histone gene expression and DNA replication was examined during the cell cycle in synchronized HeLa S3 cells. Rates of transcription were determined in vitro in isolated nuclei. A 3-5-fold increase in cell cycle dependent histone gene transcription was observed in early S phase, prior to the peak of DNA synthesis. This result is consistent with a previous determination of histone mRNA synthesis in intact cells. The transcription of these genes did not change appreciably after inhibition of DNA replication by hydroxyurea treatment, although Northern blot analysis indicated that cellular levels of histone mRNA decreased rapidly in the presence of the drug. Total cellular levels of histone mRNA closely parallel the rate of DNA synthesis as a function of cell cycle progression, reaching a maximal 20-fold increase as compared with non S phase levels. This DNA synthesis dependent accumulation of histone mRNA occurs predominantly in the cytoplasm and appears to be mediated primarily by control of histone mRNA stability. Changes in nuclear histone mRNA levels were less pronounced. These combined observations suggest that both transcriptional regulation and posttranscriptional regulation contribute toward control of the cell cycle dependent accumulation of histone mRNA during S phase, while the stability of histone mRNA throughout S phase and the selective turnover of histone mRNAs, either at the natural termination of S phase or following inhibition of DNA synthesis, are posttranscriptionally regulated

  14. Controls and Machine Protection Systems

    CERN Document Server

    Carrone, E.

    2016-01-01

    Machine protection, as part of accelerator control systems, can be managed with a 'functional safety' approach, which takes into account product life cycle, processes, quality, industrial standards and cybersafety. This paper will discuss strategies to manage such complexity and the related risks, with particular attention to fail-safe design and safety integrity levels, software and hardware standards, testing, and verification philosophy. It will also discuss an implementation of a machine protection system at the SLAC National Accelerator Laboratory's Linac Coherent Light Source (LCLS).

  15. Autonomous grain combine control system

    Science.gov (United States)

    Hoskinson, Reed L.; Kenney, Kevin L.; Lucas, James R.; Prickel, Marvin A.

    2013-06-25

    A system for controlling a grain combine having a rotor/cylinder, a sieve, a fan, a concave, a feeder, a header, an engine, and a control system. The feeder of the grain combine is engaged and the header is lowered. A separator loss target, engine load target, and a sieve loss target are selected. Grain is harvested with the lowered header passing the grain through the engaged feeder. Separator loss, sieve loss, engine load and ground speed of the grain combine are continuously monitored during the harvesting. If the monitored separator loss exceeds the selected separator loss target, the speed of the rotor/cylinder, the concave setting, the engine load target, or a combination thereof is adjusted. If the monitored sieve loss exceeds the selected sieve loss target, the speed of the fan, the size of the sieve openings, or the engine load target is adjusted.

  16. Radiation-resistant control system

    International Nuclear Information System (INIS)

    Cable, T.C.; Jones, S.

    1995-01-01

    REMOTEC has developed a open-quotes radiation resistanceclose quotes control system under a U.S. Department of Energy Small Business Innovative Research (SBIR) contract with assistance from the University of Florida. The SBIR goal was to develop a radiation resistant mobile robot from the ANDROS family of hazardous duty mobile robots that REMOTEC manufactures. See Refs. 1 and 2 for additional SBIR results. The control system, as well as the entire ANDROS robot, was redesigned, where necessary, to withstand radiation doses in excess of 10 6 rad. Those components of the robot that could not be purchased as open-quotes radiation hardenedclose quotes were tested under standard operating conditions for determination of their open-quotes radiation resistance.close quotes The entire ANDROS robot was then assembled with these new components and tested to > 10 6 rad

  17. Accelerator control systems in China

    International Nuclear Information System (INIS)

    Yao Chihyuan

    1992-01-01

    Three accelerator facilities were built in the past few years, the 2.8 GeV electron positron collider BEPC, the heavy ion SSC cyclotron accelerator HIRFL and the 800 MeV synchrotron radiation storage ring HESYRL. Aimed at different research areas, they represent a new generation of accelerator in China. This report describes the design philosophy, the structure, performance as well as future improvements of the control systems of the these facilities. (author)

  18. Learning System Center App Controller

    CERN Document Server

    Naeem, Nasir

    2015-01-01

    This book is intended for IT professionals working with Hyper-V, Azure cloud, VMM, and private cloud technologies who are looking for a quick way to get up and running with System Center 2012 R2 App Controller. To get the most out of this book, you should be familiar with Microsoft Hyper-V technology. Knowledge of Virtual Machine Manager is helpful but not mandatory.

  19. Experimental research control software system

    International Nuclear Information System (INIS)

    Cohn, I A; Kovalenko, A G; Vystavkin, A N

    2014-01-01

    A software system, intended for automation of a small scale research, has been developed. The software allows one to control equipment, acquire and process data by means of simple scripts. The main purpose of that development is to increase experiment automation easiness, thus significantly reducing experimental setup automation efforts. In particular, minimal programming skills are required and supervisors have no reviewing troubles. Interactions between scripts and equipment are managed automatically, thus allowing to run multiple scripts simultaneously. Unlike well-known data acquisition commercial software systems, the control is performed by an imperative scripting language. This approach eases complex control and data acquisition algorithms implementation. A modular interface library performs interaction with external interfaces. While most widely used interfaces are already implemented, a simple framework is developed for fast implementations of new software and hardware interfaces. While the software is in continuous development with new features being implemented, it is already used in our laboratory for automation of a helium-3 cryostat control and data acquisition. The software is open source and distributed under Gnu Public License.

  20. Experimental research control software system

    Science.gov (United States)

    Cohn, I. A.; Kovalenko, A. G.; Vystavkin, A. N.

    2014-05-01

    A software system, intended for automation of a small scale research, has been developed. The software allows one to control equipment, acquire and process data by means of simple scripts. The main purpose of that development is to increase experiment automation easiness, thus significantly reducing experimental setup automation efforts. In particular, minimal programming skills are required and supervisors have no reviewing troubles. Interactions between scripts and equipment are managed automatically, thus allowing to run multiple scripts simultaneously. Unlike well-known data acquisition commercial software systems, the control is performed by an imperative scripting language. This approach eases complex control and data acquisition algorithms implementation. A modular interface library performs interaction with external interfaces. While most widely used interfaces are already implemented, a simple framework is developed for fast implementations of new software and hardware interfaces. While the software is in continuous development with new features being implemented, it is already used in our laboratory for automation of a helium-3 cryostat control and data acquisition. The software is open source and distributed under Gnu Public License.

  1. Semantic Linkage of Control Systems

    Directory of Open Access Journals (Sweden)

    Rolf Andreas Rasenack

    2006-01-01

    Full Text Available Control systems are sets of interconnected hardware and software components which regulate the behaviour of processes. The software of modern control systems rises for some years by requirements regarding the flexibility and functionality. Thus the force of innovation grows on enterprises, since ever newer products in ever shorter time intervals must be made available. Associated hereby is the crucial shortening of the product life cycle, whose effects show up in reduced care of the software and the spares inventory. The aim, the concept presented here and developed in a modeling environment, is proved and ensures a minimum functionality of software components. Replacing software components of a control system verified for functionality by a framework at run-time and if necessary the software conditions will become adapted. Quintessential point of this implementation is the usage of an abstract syntax tree. Within its hierarchical structure meta information is attached to nodes and processed by the framework. With the development of the concept for semantic proving of software components the lifetime of software-based products is increased.

  2. Mindfulness-Based Stress Release Program for University Employees: A Pilot, Waitlist-Controlled Trial and Implementation Replication.

    Science.gov (United States)

    Koncz, Rebecca; Wolfenden, Fiona; Hassed, Craig; Chambers, Richard; Cohen, Julia; Glozier, Nicholas

    2016-10-01

    The aim of this study was to evaluate the effectiveness of a 6-week mindfulness-based stress release program (SRP) on stress and work engagement in fulltime university employees. Perceived stress, workplace wellbeing, and engagement were measured at baseline and within 1 week of the SRP completion, and contemporaneously 6 weeks apart for a waitlist control group. A second program was implemented to examine reproducibility of results. Fifty participants undertook the SRPs, and 29 participants were waitlisted. A significant improvement in distress, workplace wellbeing, and vigor was observed within the first SRP group, when compared with the control group. The improvement in distress and wellbeing was reproduced in the second SRP group. This study adds to the growing body of research that mindfulness may be an effective method for reducing workplace stress, improving employee wellbeing, and enhancing work engagement.

  3. Replication of Merkel cell polyomavirus induces reorganization of promyelocytic leukemia nuclear bodies.

    Science.gov (United States)

    Neumann, Friederike; Czech-Sioli, Manja; Dobner, Thomas; Grundhoff, Adam; Schreiner, Sabrina; Fischer, Nicole

    2016-11-01

    Merkel cell polyomavirus (MCPyV) is associated with Merkel cell carcinoma (MCC), a rare but aggressive skin cancer. The virus is highly prevalent: 60-80 % of adults are seropositive; however, cells permissive for MCPyV infection are unknown. Consequently, very little information about the MCPyV life cycle is available. Until recently, MCPyV replication could only be studied using a semi-permissive in vitro replication system (Neumann et al., 2011; Feng et al., 2011, Schowalter et al., 2011). MCPyV replication most likely depends on subnuclear structures such as promyelocytic leukemia protein nuclear bodies (PML-NBs), which are known to play regulatory roles in the infection of many DNA viruses. Here, we investigated PML-NB components as candidate host factors to control MCPyV DNA replication. We showed that PML-NBs change in number and size in cells actively replicating MCPyV proviral DNA. We observed a significant increase in PML-NBs in cells positive for MCPyV viral DNA replication. Interestingly, a significant amount of cells actively replicating MCPyV did not show any Sp100 expression. While PML and Daxx had no effect on MCPyV DNA replication, MCPyV replication was increased in cells depleted for Sp100, strongly suggesting that Sp100 is a negative regulator of MCPyV DNA replication.

  4. The Fermilab Accelerator control system

    Science.gov (United States)

    Bogert, Dixon

    1986-06-01

    With the advent of the Tevatron, considerable upgrades have been made to the controls of all the Fermilab Accelerators. The current system is based on making as large an amount of data as possible available to many operators or end-users. Specifically there are about 100 000 separate readings, settings, and status and control registers in the various machines, all of which can be accessed by seventeen consoles, some in the Main Control Room and others distributed throughout the complex. A "Host" computer network of approximately eighteen PDP-11/34's, seven PDP-11/44's, and three VAX-11/785's supports a distributed data acquisition system including Lockheed MAC-16's left from the original Main Ring and Booster instrumentation and upwards of 1000 Z80, Z8002, and M68000 microprocessors in dozens of configurations. Interaction of the various parts of the system is via a central data base stored on the disk of one of the VAXes. The primary computer-hardware communication is via CAMAC for the new Tevatron and Antiproton Source; certain subsystems, among them vacuum, refrigeration, and quench protection, reside in the distributed microprocessors and communicate via GAS, an in-house protocol. An important hardware feature is an accurate clock system making a large number of encoded "events" in the accelerator supercycle available for both hardware modules and computers. System software features include the ability to save the current state of the machine or any subsystem and later restore it or compare it with the state at another time, a general logging facility to keep track of specific variables over long periods of time, detection of "exception conditions" and the posting of alarms, and a central filesharing capability in which files on VAX disks are available for access by any of the "Host" processors.

  5. The Fermilab accelerator control system

    International Nuclear Information System (INIS)

    Bogert, D.

    1986-01-01

    With the advent of the Tevatron, considerable upgrades have been made to the controls of all the Fermilab Accelerators. The current system is based on making as large an amount of data as possible available to many operators or end-users. Specifically there are about 100000 separate readings, settings, and status and control registers in the various machines, all of which can be accessed by seventeen consoles, some in the Main Control Room and others distributed throughout the complex. A ''Host'' computer network of approximately eighteen PDP-11/34's, seven PDP-11/44's, and three VAX-11/785's supports a distributed data acquisition system including Lockheed MAC-16's left from the original Main Ring and Booster instrumentation and upwards of 1000 Z80, Z8002, and M68000 microprocessors in dozens of configurations. Interaction of the various parts of the system is via a central data base stored on the disk of one of the VAXes. The primary computer-hardware communication is via CAMAC for the new Tevatron and Antiproton Source; certain subsystems, among them vacuum, refrigeration and quench protection, reside in the distributed microprocessors and communicate via GAS, an in-house protocol. An important hardware feature is an accurate clock system making a large number of encoded ''events'' in the accelerator supercycle available for both hardware modules and computers. System software features include the ability to save the current state of the machine or any subsystem and later restore it or compare it with the state at another time, a general logging facility to keep track of specific variables over long periods of time, detection of 'exception conditions' and the posting of alarms, and a central filesharing capability in which files on VAX disks are available for access by any of the ''Host'' processors. (orig.)

  6. Synchrotron control system of the HIMAC

    International Nuclear Information System (INIS)

    Takada, E.; Sato, K.; Itano, A.

    1994-01-01

    A structural design synopsis and the present status of the HIMAC synchrotron control system are described. The control system comprises of Timing System, (ring magnet) Power-supply Controller, Programmable Logic Controller, Static Var Compensator controller, Monitor Controller, RF control computer, Beam Transport control computer and the synchrotron main computer (denoted as CS, hereafter) that forms a local area cluster with man-machine interfacing computers, and communicates with HIMAC supervisor computer. (author)

  7. HTGR Resilient Control System Strategy

    International Nuclear Information System (INIS)

    Stevens, Lynne M.

    2010-01-01

    A preeminent objective for corporate and government organizations is the protection of major investments, which is attained by achieving state awareness, a comprehensive understanding of security and safety, for critical infrastructures. Given the dependence of critical infrastructure on control systems for automation, the integrity of these systems and their ability to provide owner/operators a high degree of state awareness is essential in attaining a high degree of investment protection and public acceptance. Operators as well as government are therefore burdened to ensure they have a timely understanding of the status of their plant or all plants, respectively, to ensure efficient operations and investment and public protection. 'This characterization is a significant objective that must consider many aspects of instrumentation, control, and intelligent systems in order to achieve the required result. These aspects include sensory, communication, analysis, decision, and human system interfaces necessary to achieve fusion of data and presentation of results that will provide an understanding of what issues are important and why.

  8. HTGR Resilient Control System Strategy

    Energy Technology Data Exchange (ETDEWEB)

    Lynne M. Stevens

    2010-09-01

    A preeminent objective for corporate and government organizations is the protection of major investments, which is attained by achieving state awareness, a comprehensive understanding of security and safety, for critical infrastructures. Given the dependence of critical infrastructure on control systems for automation, the integrity of these systems and their ability to provide owner/operators a high degree of state awareness is essential in attaining a high degree of investment protection and public acceptance. Operators as well as government are therefore burdened to ensure they have a timely understanding of the status of their plant or all plants, respectively, to ensure efficient operations and investment and public protection. “This characterization is a significant objective that must consider many aspects of instrumentation, control, and intelligent systems in order to achieve the required result. These aspects include sensory, communication, analysis, decision, and human system interfaces necessary to achieve fusion of data and presentation of results that will provide an understanding of what issues are important and why.

  9. Effector-Triggered Self-Replication in Coupled Subsystems

    NARCIS (Netherlands)

    Komáromy, Dávid; Tezcan, Meniz; Schaeffer, Gaël; Marić, Ivana; Otto, Sijbren

    2017-01-01

    In living systems processes like genome duplication and cell division are carefully synchronized through subsystem coupling. If we are to create life de novo, similar control over essential processes such as self-replication need to be developed. Here we report that coupling two dynamic

  10. Surface micro topography replication in injection moulding

    DEFF Research Database (Denmark)

    Arlø, Uffe Rolf

    Thermoplastic injection moulding is a widely used industrial process that involves surface generation by replication. The surface topography of injection moulded plastic parts can be important for aesthetical or technical reasons. With the emergence of microengineering and nanotechnology additional...... importance of surface topography follows. In general the replication is not perfect and the topography of the plastic part differs from the inverse topography of the mould cavity. It is desirable to be able to control the degree of replication perfection or replication quality. This requires an understanding...... of the physical mechanisms of replication. Such understanding can lead to improved process design and facilitate in-line process quality control with respect to surface properties. The purpose of the project is to identify critical factors that affect topography replication quality and to obtain an understanding...

  11. Intelligent control-III: fuzzy control system

    International Nuclear Information System (INIS)

    Nagrial, M.H.

    2004-01-01

    During the last decade or so, fuzzy logic control (FLC) has emerged as one of the most active and fruitful areas of research and development. The applications include industrial process control to medical diagnostic and financial markets. Many consumer products using this technology are available in the market place. FLC is best suited to complex ill-defined processes that can be controlled by a skilled human operator without much knowledge of their underlying dynamics. This lecture will cover the basic architecture and the design methodology of fuzzy logic controllers. FLC will be strongly based on the concepts of fuzzy set theory, introduced in first lecture. Some practical applications will also be discussed and presented. (author)

  12. Integration of Fire Control, Flight Control and Propulsion Control Systems.

    Science.gov (United States)

    1983-08-01

    pourront etre enploy6s. Avant l’attaque, des profils de vole contr~lant I’Anergie seront 6tablis pour augmenter au maximum l’dnergie disponible do...VERIIATION PROCEDURES NTRTO PERFORM CONTROL SYSTEM REPEAT TESTS WITH ACTUAL ENGIRE ITEATO INTEGRATION TO TIENMO. RUUINED TE VALIDATE TESTING TESI ...fonrtionnement est disponible . Teat-dea rodur d’nrf /sortie .an. scs i lquiesent posslde ce* deux dispositife, on test des rodeurs et sx~fiutA *Sur une vote do

  13. Self-controlled feedback is effective if it is based on the learner's performance: a replication and extension of Chiviacowsky and Wulf (2005).

    Science.gov (United States)

    Carter, Michael J; Carlsen, Anthony N; Ste-Marie, Diane M

    2014-01-01

    The learning advantages of self-controlled feedback schedules compared to yoked schedules have been attributed to motivational influences and/or information processing activities with many researchers adopting the motivational perspective in recent years. Chiviacowsky and Wulf (2005) found that feedback decisions made before (Self-Before) or after a trial (Self-After) resulted in similar retention performance, but superior transfer performance resulted when the decision to receive feedback occurred after a trial. They suggested that the superior skill transfer of the Self-After group likely emerged from information processing activities such as error estimation. However, the lack of yoked groups and a measure of error estimation in their experimental design prevents conclusions being made regarding the underlying mechanisms of why self-controlled feedback schedules optimize learning. Here, we revisited Chiviacowsky and Wulf's (2005) design to investigate the learning benefits of self-controlled feedback schedules. We replicated their Self-Before and Self-After groups, but added a Self-Both group that was able to request feedback before a trial, but could then change or stay with their original choice after the trial. Importantly, yoked groups were included for the three self-controlled groups to address the previously stated methodological limitation and error estimations were included to examine whether self-controlling feedback facilitates a more accurate error detection and correction mechanism. The Self-After and Self-Before groups demonstrated similar accuracy in physical performance and error estimation scores in retention and transfer, and both groups were significantly more accurate than the Self-Before group and their respective Yoked groups (p's 0.05). We suggest these findings further indicate that informational factors associated with the processing of feedback for the development of one's error detection and correction mechanism, rather than

  14. Switching Systems: Controllability and Control Design

    Science.gov (United States)

    2009-04-25

    both continuous and discrete dynamics, are abundant in essentially all areas of engineering and scientific endeavor. Hybrid systems can switch between...TERMS EOARD, Navigation, Comunications & Guidance, Complex Systems 16. SECURITY CLASSIFICATION OF: 19a. NAME OF RESPONSIBLE PERSON JAMES LAWTON Ph

  15. Instrumentation, Control, and Intelligent Systems

    Energy Technology Data Exchange (ETDEWEB)

    2005-09-01

    Abundant and affordable energy is required for U.S. economic stability and national security. Advanced nuclear power plants offer the best near-term potential to generate abundant, affordable, and sustainable electricity and hydrogen without appreciable generation of greenhouse gases. To that end, Idaho National Laboratory (INL) has been charged with leading the revitalization of nuclear power in the U.S. The INL vision is to become the preeminent nuclear energy laboratory with synergistic, world-class, multi-program capabilities and partnerships by 2015. The vision focuses on four essential destinations: (1) Be the preeminent internationally-recognized nuclear energy research, development, and demonstration laboratory; (2) Be a major center for national security technology development and demonstration; (3) Be a multi-program national laboratory with world-class capabilities; (4) Foster academic, industry, government, and international collaborations to produce the needed investment, programs, and expertise. Crucial to that effort is the inclusion of research in advanced instrumentation, control, and intelligent systems (ICIS) for use in current and advanced power and energy security systems to enable increased performance, reliability, security, and safety. For nuclear energy plants, ICIS will extend the lifetime of power plant systems, increase performance and power output, and ensure reliable operation within the system's safety margin; for national security applications, ICIS will enable increased protection of our nation's critical infrastructure. In general, ICIS will cost-effectively increase performance for all energy security systems.

  16. Instrumentation, Control, and Intelligent Systems

    International Nuclear Information System (INIS)

    Not Available

    2005-01-01

    Abundant and affordable energy is required for U.S. economic stability and national security. Advanced nuclear power plants offer the best near-term potential to generate abundant, affordable, and sustainable electricity and hydrogen without appreciable generation of greenhouse gases. To that end, Idaho National Laboratory (INL) has been charged with leading the revitalization of nuclear power in the U.S. The INL vision is to become the preeminent nuclear energy laboratory with synergistic, world-class, multi-program capabilities and partnerships by 2015. The vision focuses on four essential destinations: (1) Be the preeminent internationally-recognized nuclear energy research, development, and demonstration laboratory; (2) Be a major center for national security technology development and demonstration; (3) Be a multi-program national laboratory with world-class capabilities; (4) Foster academic, industry, government, and international collaborations to produce the needed investment, programs, and expertise. Crucial to that effort is the inclusion of research in advanced instrumentation, control, and intelligent systems (ICIS) for use in current and advanced power and energy security systems to enable increased performance, reliability, security, and safety. For nuclear energy plants, ICIS will extend the lifetime of power plant systems, increase performance and power output, and ensure reliable operation within the system's safety margin; for national security applications, ICIS will enable increased protection of our nation's critical infrastructure. In general, ICIS will cost-effectively increase performance for all energy security systems

  17. Architecture and inherent robustness of a bacterial cell-cycle control system.

    Science.gov (United States)

    Shen, Xiling; Collier, Justine; Dill, David; Shapiro, Lucy; Horowitz, Mark; McAdams, Harley H

    2008-08-12

    A closed-loop control system drives progression of the coupled stalked and swarmer cell cycles of the bacterium Caulobacter crescentus in a near-mechanical step-like fashion. The cell-cycle control has a cyclical genetic circuit composed of four regulatory proteins with tight coupling to processive chromosome replication and cell division subsystems. We report a hybrid simulation of the coupled cell-cycle control system, including asymmetric cell division and responses to external starvation signals, that replicates mRNA and protein concentration patterns and is consistent with observed mutant phenotypes. An asynchronous sequential digital circuit model equivalent to the validated simulation model was created. Formal model-checking analysis of the digital circuit showed that the cell-cycle control is robust to intrinsic stochastic variations in reaction rates and nutrient supply, and that it reliably stops and restarts to accommodate nutrient starvation. Model checking also showed that mechanisms involving methylation-state changes in regulatory promoter regions during DNA replication increase the robustness of the cell-cycle control. The hybrid cell-cycle simulation implementation is inherently extensible and provides a promising approach for development of whole-cell behavioral models that can replicate the observed functionality of the cell and its responses to changing environmental conditions.

  18. DNA replication and cancer: From dysfunctional replication origin activities to therapeutic opportunities.

    Science.gov (United States)

    Boyer, Anne-Sophie; Walter, David; Sørensen, Claus Storgaard

    2016-06-01

    A dividing cell has to duplicate its DNA precisely once during the cell cycle to preserve genome integrity avoiding the accumulation of genetic aberrations that promote diseases such as cancer. A large number of endogenous impacts can challenge DNA replication and cells harbor a battery of pathways to promote genome integrity during DNA replication. This includes suppressing new replication origin firing, stabilization of replicating forks, and the safe restart of forks to prevent any loss of genetic information. Here, we describe mechanisms by which oncogenes can interfere with DNA replication thereby causing DNA replication stress and genome instability. Further, we describe cellular and systemic responses to these insults with a focus on DNA replication restart pathways. Finally, we discuss the therapeutic potential of exploiting intrinsic replicative stress in cancer cells for targeted therapy. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. About development of automation control systems

    Science.gov (United States)

    Myshlyaev, L. P.; Wenger, K. G.; Ivushkin, K. A.; Makarov, V. N.

    2018-05-01

    The shortcomings of approaches to the development of modern control automation systems and ways of their improvement are given: the correct formation of objects for study and optimization; a joint synthesis of control objects and control systems, an increase in the structural diversity of the elements of control systems. Diagrams of control systems with purposefully variable structure of their elements are presented. Structures of control algorithms for an object with a purposefully variable structure are given.

  20. Design and Architecture of SST-1 basic plasma control system

    Energy Technology Data Exchange (ETDEWEB)

    Patel, Kirit, E-mail: kpatel@ipr.res.in; Raju, D.; Dhongde, J.; Mahajan, K.; Chudasama, H.; Gulati, H.; Chauhan, A.; Masand, H.; Bhandarkar, M.; Pradhan, S.

    2016-11-15

    Highlights: • Reflective Memory network. • FPAG based Timing system for trigger distribution. • IRIG-B network for GPS time synchronization. • PMC based Digital Signal Processors and VME. • Simultaneous sampling ADC. - Abstract: Primary objective of SST-1 Plasma control system is to achieve Plasma position, shape and current profile control. Architecture of control system for SST-1 is distributed in nature. Fastest control loop time requirement of 100 μs is achieved using VME based simultaneous sampling ADCs, PMC based quad core DSP, Reflective Memory [RFM] based real-time network, VME based real-time trigger distribution network and Ethernet network. All the control loops for shape control, position control and current profile control share common signals from Magnetic diagnostic so it is planned to accommodate all the algorithms on the same PMC based quad core DSP module TS C-43. RFM based real-time data network replicate data from one node to next node in a ring network topology at sustained throughput rate of 13.4 MBps. Real-time Timing System network provides guaranteed trigger distribution in 3.8 μs from one node to all node of the network. Monitoring and configuration of different systems participating in the operation of SST-1 is done by Ethernet network. Magnetic sensors data is acquired using Pentek 6802 simultaneously sampling ADC card at the rate of 10KSPS. All the real-time raw data along with the control data will be archived using RFM network and SCSI HDD for the experiment duration of 1000 s. RFM network is also planned for real-time plotting of key parameter of Plasma during long experiment. After experiment this data is transferred to central storage server for archival purpose. This paper discusses the architecture and hardware implementation of the control system by describing all the involved hardware and software along with future plans for up-gradations.

  1. Superior control of HIV-1 replication by CD8+ T cells targeting conserved epitopes: implications for HIV vaccine design.

    Directory of Open Access Journals (Sweden)

    Pratima Kunwar

    Full Text Available A successful HIV vaccine will likely induce both humoral and cell-mediated immunity, however, the enormous diversity of HIV has hampered the development of a vaccine that effectively elicits both arms of the adaptive immune response. To tackle the problem of viral diversity, T cell-based vaccine approaches have focused on two main strategies (i increasing the breadth of vaccine-induced responses or (ii increasing vaccine-induced responses targeting only conserved regions of the virus. The relative extent to which set-point viremia is impacted by epitope-conservation of CD8(+ T cell responses elicited during early HIV-infection is unknown but has important implications for vaccine design. To address this question, we comprehensively mapped HIV-1 CD8(+ T cell epitope-specificities in 23 ART-naïve individuals during early infection and computed their conservation score (CS by three different methods (prevalence, entropy and conseq on clade-B and group-M sequence alignments. The majority of CD8(+ T cell responses were directed against variable epitopes (p<0.01. Interestingly, increasing breadth of CD8(+ T cell responses specifically recognizing conserved epitopes was associated with lower set-point viremia (r = - 0.65, p = 0.009. Moreover, subjects possessing CD8(+ T cells recognizing at least one conserved epitope had 1.4 log10 lower set-point viremia compared to those recognizing only variable epitopes (p = 0.021. The association between viral control and the breadth of conserved CD8(+ T cell responses may be influenced by the method of CS definition and sequences used to determine conservation levels. Strikingly, targeting variable versus conserved epitopes was independent of HLA type (p = 0.215. The associations with viral control were independent of functional avidity of CD8(+ T cell responses elicited during early infection. Taken together, these data suggest that the next-generation of T-cell based HIV-1 vaccines should focus

  2. Requirements for implementing real-time control functional modules on a hierarchical parallel pipelined system

    Science.gov (United States)

    Wheatley, Thomas E.; Michaloski, John L.; Lumia, Ronald

    1989-01-01

    Analysis of a robot control system leads to a broad range of processing requirements. One fundamental requirement of a robot control system is the necessity of a microcomputer system in order to provide sufficient processing capability.The use of multiple processors in a parallel architecture is beneficial for a number of reasons, including better cost performance, modular growth, increased reliability through replication, and flexibility for testing alternate control strategies via different partitioning. A survey of the progression from low level control synchronizing primitives to higher level communication tools is presented. The system communication and control mechanisms of existing robot control systems are compared to the hierarchical control model. The impact of this design methodology on the current robot control systems is explored.

  3. On the equivalence of chaos control systems

    International Nuclear Information System (INIS)

    Wang Xiaofan

    2003-01-01

    For a given chaotic system, different control systems can be constructed depending on which parameter is tuned or where the external input is added. We prove that two different feedback control systems are qualitatively equivalent if they are feedback linearizable

  4. Study on Design of Control Module and Fuzzy Control System

    International Nuclear Information System (INIS)

    Lee, Chang Kyu; Sohn, Chang Ho; Kim, Jung Seon; Kim, Min Kyu

    2005-01-01

    Performance of control unit is improved by introduction of fuzzy control theory and compensation for input of control unit as FLC(Fuzzy Logic Controller). Here, FLC drives thermal control system by linguistic rule-base. Hence, In case of using compensative PID control unit, it doesn't need to revise or compensate for PID control unit. Consequently, this study shows proof that control system which implements H/W module and then uses fuzzy algorism in this system is stable and has reliable performance

  5. Almaraz ovation control system security

    Energy Technology Data Exchange (ETDEWEB)

    Madronal Rodriguez, E.; Anderson, E.; Jimenez Diaz, J.; Carrasco Mateos, J. A.

    2013-07-01

    Improving the security of a plant's Distributed Control System (DCS) is an important consideration for plant safety and profitability, as well as the necessity to comply with the regulation. The U.S. Nuclear Regulatory Commission has produced Regulatory Guide (RG) 5.71, and the Nuclear Energy Institute (NEI) has produced NEI 08-09 to assist plants in meeting 10 CFR 73.54, Protection of digital computer and communication systems and networks. These requirements, which address the establishment, implementation and maintenance of a cyber security program, present challenges to ensure that safety, security and emergency preparedness functions of nuclear facilities are not negatively impacted by the vulnerability scanning and testing process.

  6. Almaraz ovation control system security

    International Nuclear Information System (INIS)

    Madronal Rodriguez, E.; Anderson, E.; Jimenez Diaz, J.; Carrasco Mateos, J. A.

    2013-01-01

    Improving the security of a plant's Distributed Control System (DCS) is an important consideration for plant safety and profitability, as well as the necessity to comply with the regulation. The U.S. Nuclear Regulatory Commission has produced Regulatory Guide (RG) 5.71, and the Nuclear Energy Institute (NEI) has produced NEI 08-09 to assist plants in meeting 10 CFR 73.54, Protection of digital computer and communication systems and networks. These requirements, which address the establishment, implementation and maintenance of a cyber security program, present challenges to ensure that safety, security and emergency preparedness functions of nuclear facilities are not negatively impacted by the vulnerability scanning and testing process.

  7. Advances and applications in nonlinear control systems

    CERN Document Server

    Volos, Christos

    2016-01-01

    The book reports on the latest advances and applications of nonlinear control systems. It consists of 30 contributed chapters by subject experts who are specialized in the various topics addressed in this book. The special chapters have been brought out in the broad areas of nonlinear control systems such as robotics, nonlinear circuits, power systems, memristors, underwater vehicles, chemical processes, observer design, output regulation, backstepping control, sliding mode control, time-delayed control, variables structure control, robust adaptive control, fuzzy logic control, chaos, hyperchaos, jerk systems, hyperjerk systems, chaos control, chaos synchronization, etc. Special importance was given to chapters offering practical solutions, modeling and novel control methods for the recent research problems in nonlinear control systems. This book will serve as a reference book for graduate students and researchers with a basic knowledge of electrical and control systems engineering. The resulting design proce...

  8. Architecture and control of a high current ion implanter system

    International Nuclear Information System (INIS)

    Bayer, E.H.; Paul, L.F.; Kranik, J.R.

    1979-01-01

    The design of an ion implant system for use in production requires that special attention be given to areas of design which normally are not emphasized on research or development type ion implanters. Manually operated, local controls are replaced by remote controls, automatic sequencing, and digital displays. For ease of maintenance and replication the individual components are designed as simply as possible and are contained in modules of separate identities, joined only by the beam line and electrical interconnections. A production environment also imposes requirements for the control of contamination and maintainability of clean room integrity. For that reason the major portion of the hardware is separated from the clean operator area and is housed in a maintenance core area. The controls of a production system should also be such that relatively unskilled technicians are able to operate the system with optimum repeatability and minimum operator intervention. An extensive interlock system is required. Most important, for use in production the ion implant system has to have a relatively high rate of throughput. Since the rate of throughput at a given dose is a function of beam current, pumpdown time and wafer handling capacity, design of components affecting these parameters has been optimized. Details of the system are given. (U.K.)

  9. Independent control of replication initiation of the two Vibrio cholerae chromosomes by DnaA and RctB

    DEFF Research Database (Denmark)

    Duigou, Stephane; Knudsen, Kristine Groth; Skovgaard, Ole

    2006-01-01

    Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a...

  10. SUMO and KSHV Replication

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Pei-Ching [Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 112, Taiwan (China); Kung, Hsing-Jien, E-mail: hkung@nhri.org.tw [Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan (China); Department of Biochemistry and Molecular Medicine, University of California, Davis, CA 95616 (United States); UC Davis Cancer Center, University of California, Davis, CA 95616 (United States); Division of Molecular and Genomic Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli County 35053, Taiwan (China)

    2014-09-29

    Small Ubiquitin-related MOdifier (SUMO) modification was initially identified as a reversible post-translational modification that affects the regulation of diverse cellular processes, including signal transduction, protein trafficking, chromosome segregation, and DNA repair. Increasing evidence suggests that the SUMO system also plays an important role in regulating chromatin organization and transcription. It is thus not surprising that double-stranded DNA viruses, such as Kaposi’s sarcoma-associated herpesvirus (KSHV), have exploited SUMO modification as a means of modulating viral chromatin remodeling during the latent-lytic switch. In addition, SUMO regulation allows the disassembly and assembly of promyelocytic leukemia protein-nuclear bodies (PML-NBs), an intrinsic antiviral host defense, during the viral replication cycle. Overcoming PML-NB-mediated cellular intrinsic immunity is essential to allow the initial transcription and replication of the herpesvirus genome after de novo infection. As a consequence, KSHV has evolved a way as to produce multiple SUMO regulatory viral proteins to modulate the cellular SUMO environment in a dynamic way during its life cycle. Remarkably, KSHV encodes one gene product (K-bZIP) with SUMO-ligase activities and one gene product (K-Rta) that exhibits SUMO-targeting ubiquitin ligase (STUbL) activity. In addition, at least two viral products are sumoylated that have functional importance. Furthermore, sumoylation can be modulated by other viral gene products, such as the viral protein kinase Orf36. Interference with the sumoylation of specific viral targets represents a potential therapeutic strategy when treating KSHV, as well as other oncogenic herpesviruses. Here, we summarize the different ways KSHV exploits and manipulates the cellular SUMO system and explore the multi-faceted functions of SUMO during KSHV’s life cycle and pathogenesis.

  11. Development of controlled drilling system

    International Nuclear Information System (INIS)

    Kiho, Kenzo; Miyakawa, Kimio; Suzuki, Koichi; Sunaga, Takayuki

    2008-01-01

    In Japan, the soft sedimentary rock of the Neogene tertiary is being focused as a host rock for the High Level Radioactive Waste (HLW) disposal. Especially, the soft sedimentary rock at the offshore, region is thought to be one of the best candidates, since there is no driving force of the underground water. The measurement and logging in the bore hole in order to check the hydro-geological and geomechanical conditions of the host rock is a very important way to examine the potentially of the disposal candidates. The CRIEPI (Central Research Institute of Electric Power Industry) has been conducting the project about the controlled drilling technology and the measurement and logging technologies in its borehole. In 2000, as the beginning year of the project, we made the conceptual design of the drilling and measuring systems, and made key tools concerning each technology on an experimental basis. We have been developing sub tools constructing drilling and measuring systems since 2000, and applying these systems to the Horonobe site recent 5 years. We will briefly report the outline of the system and the results of drilling and measurement that were carried out at the Horonobe site. (author)

  12. The role of IREB2 and transforming growth factor beta-1 genetic variants in COPD: a replication case-control study

    LENUS (Irish Health Repository)

    Chappell, Sally L

    2011-02-14

    Abstract Background Genetic factors are known to contribute to COPD susceptibility and these factors are not fully understood. Conflicting results have been reported for many genetic studies of candidate genes based on their role in the disease. Genome-wide association studies in combination with expression profiling have identified a number of new candidates including IREB2. A meta-analysis has implicated transforming growth factor beta-1 (TGFbeta1) as a contributor to disease susceptibility. Methods We have examined previously reported associations in both genes in a collection of 1017 white COPD patients and 912 non-diseased smoking controls. Genotype information was obtained for seven SNPs in the IREB2 gene, and for four SNPs in the TGFbeta1 gene. Allele and genotype frequencies were compared between COPD cases and controls, and odds ratios were calculated. The analysis was adjusted for age, sex, smoking and centre, including interactions of age, sex and smoking with centre. Results Our data replicate the association of IREB2 SNPs in association with COPD for SNP rs2568494, rs2656069 and rs12593229 with respective adjusted p-values of 0.0018, 0.0039 and 0.0053. No significant associations were identified for TGFbeta1. Conclusions These studies have therefore confirmed that the IREB2 locus is a contributor to COPD susceptibility and suggests a new pathway in COPD pathogenesis invoking iron homeostasis.

  13. Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins

    DEFF Research Database (Denmark)

    Foulk, M. S.; Urban, J. M.; Casella, Cinzia

    2015-01-01

    Nascent strand sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification of features for their specification. NS-seq depends on the ability of lambda exonuclease (lambda-exo) to efficiently digest parental DNA while leaving RNA-primer protected nascent...... strands intact. We used genomics and biochemical approaches to determine if lambda-exo digests all parental DNA sequences equally. We report that lambda-exo does not efficiently digest G-quadruplex (G4) structures in a plasmid. Moreover, lambda-exo digestion of nonreplicating genomic DNA (LexoG0) enriches...... GC-rich DNA and G4 motifs genome-wide. We used LexoG0 data to control for nascent strand-independent lambda-exo biases in NSseq and validated this approach at the rDNA locus. The lambda-exo-controlled NS-seq peaks are not GC-rich, and only 35.5% overlap with 6.8% of all G4s, suggesting that G4s...

  14. Autonomous intelligent cruise control system

    Science.gov (United States)

    Baret, Marc; Bomer, Thierry T.; Calesse, C.; Dudych, L.; L'Hoist, P.

    1995-01-01

    Autonomous intelligent cruise control (AICC) systems are not only controlling vehicles' speed but acting on the throttle and eventually on the brakes they could automatically maintain the relative speed and distance between two vehicles in the same lane. And more than just for comfort it appears that these new systems should improve the safety on highways. By applying a technique issued from the space research carried out by MATRA, a sensor based on a charge coupled device (CCD) was designed to acquire the reflected light on standard-mounted car reflectors of pulsed laser diodes emission. The CCD is working in a unique mode called flash during transfer (FDT) which allows identification of target patterns in severe optical environments. It provides high accuracy for distance and angular position of targets. The absence of moving mechanical parts ensures high reliability for this sensor. The large field of view and the high measurement rate give a global situation assessment and a short reaction time. Then, tracking and filtering algorithms have been developed in order to select the target, on which the equipped vehicle determines its safety distance and speed, taking into account its maneuvering and the behaviors of other vehicles.

  15. Control System for Prosthetic Devices

    Science.gov (United States)

    Bozeman, Richard J. (Inventor)

    1996-01-01

    A control system and method for prosthetic devices is provided. The control system comprises a transducer for receiving movement from a body part for generating a sensing signal associated with that of movement. The sensing signal is processed by a linearizer for linearizing the sensing signal to be a linear function of the magnitude of the distance moved by the body part. The linearized sensing signal is normalized to be a function of the entire range of body part movement from the no-shrug position of the moveable body part through the full-shrg position of the moveable body part. The normalized signal is divided into a plurality of discrete command signals. The discrete command signals are used by typical converter devices which are in operational association with the prosthetic device. The converter device uses the discrete command signals for driving the moveable portions of the prosthetic device and its sub-prosthesis. The method for controlling a prosthetic device associated with the present invention comprises the steps of receiving the movement from the body part, generating a sensing signal in association with the movement of the body part, linearizing the sensing signal to be a linear function of the magnitude of the distance moved by the body part, normalizing the linear signal to be a function of the entire range of the body part movement, dividing the normalized signal into a plurality of discrete command signals, and implementing the plurality of discrete command signals for driving the respective moveable prosthesis device and its sub-prosthesis.

  16. Automatic control system at the ''Loviisa'' NPP

    International Nuclear Information System (INIS)

    Kukhtevich, I.V.; Mal'tsev, B.K.; Sergievskaya, E.N.

    1980-01-01

    Automatic control system of the Loviisa-1 NPP (Finland) is described. According to operation conditions of Finland power system the Loviisa-1 NPP must operate in the mode of week and day control of loading schedule and participate in current control of power system frequency and capacity. With provision for these requirements NPP is equipped with the all-regime system for automatic control functioning during reactor start-up, shut-down, in normal and transient regimes and in emergency situations. The automatic control system includes: a data subsystem, an automatic control subsystem, a discrete control subsystem including remote, a subsystem for reactor control and protection and overall station system of protections: control and dosimetry inside the reactor. Structures of a data-computer complex, discrete control subsystems, reactor control and protection systems, neutron flux control system, inside-reactor control system, station protection system and system for control of fuel element tightness are presented in short. Two-year experience of the NPP operation confirmed advisability of the chosen volume of automatization. The Loviisa-1 NPP operates successfully in the mode of the week and day control of supervisor schedule and current control of frequency (short-term control)

  17. Live Replication of Paravirtual Machines

    OpenAIRE

    Stodden, Daniel

    2009-01-01

    Virtual machines offer a fair degree of system state encapsulation, which promotes practical advances in fault tolerance, system debugging, profiling and security applications. This work investigates deterministic replay and semi-active replication for system paravirtualization, a software discipline trading guest kernel binar compatibility for reduced dependency on costly trap-and-emulate techniques. A primary contribution is evidence that trace capturing under a piecewise deterministic exec...

  18. Central control system for the EAST tokamak

    International Nuclear Information System (INIS)

    Sun Xiaoyang; Ji Zhenshan; Wu Yicun; Luo Jiarong

    2008-01-01

    The architecture, the main function and the design scheme of the central control system and the collaboration system of EAST tokamak are described. The main functions of the central control system are to supply a union control interface for all the control, diagnoses, and data acquisition (DAQ) subsystem and it is also designed to synchronize all those subsystem. (authors)

  19. Insulated hsp70B' promoter: stringent heat-inducible activity in replication-deficient, but not replication-competent adenoviruses.

    Science.gov (United States)

    Rohmer, Stanimira; Mainka, Astrid; Knippertz, Ilka; Hesse, Andrea; Nettelbeck, Dirk M

    2008-04-01

    Key to the realization of gene therapy is the development of efficient and targeted gene transfer vectors. Therapeutic gene transfer by replication-deficient or more recently by conditionally replication-competent/oncolytic adenoviruses has shown much promise. For specific applications, however, it will be advantageous to provide vectors that allow for external control of gene expression. The efficient cellular heat shock system in combination with available technology for focused and controlled hyperthermia suggests heat-regulated transcription control as a promising tool for this purpose. We investigated the feasibility of a short fragment of the human hsp70B' promoter, with and without upstream insulator elements, for the regulation of transgene expression by replication-deficient or oncolytic adenoviruses. Two novel adenoviral vectors with an insulated hsp70B' promoter were developed and showed stringent heat-inducible gene expression with induction ratios up to 8000-fold. In contrast, regulation of gene expression from the hsp70B' promoter without insulation was suboptimal. In replication-competent/oncolytic adenoviruses regulation of the hsp70B' promoter was lost specifically during late replication in permissive cells and could not be restored by the insulators. We developed novel adenovirus gene transfer vectors that feature improved and stringent regulation of transgene expression from the hsp70B' promoter using promoter insulation. These vectors have potential for gene therapy applications that benefit from external modulation of therapeutic gene expression or for combination therapy with hyperthermia. Furthermore, our study reveals that vector replication can deregulate inserted cellular promoters, an observation which is of relevance for the development of replication-competent/oncolytic gene transfer vectors. (c) 2008 John Wiley & Sons, Ltd.

  20. Changes in Pore Water Quality After Peatland Restoration: Assessment of a Large-Scale, Replicated Before-After-Control-Impact Study in Finland

    Science.gov (United States)

    Menberu, Meseret Walle; Marttila, Hannu; Tahvanainen, Teemu; Kotiaho, Janne S.; Hokkanen, Reijo; Kløve, Bjørn; Ronkanen, Anna-Kaisa

    2017-10-01

    Drainage is known to affect peatland natural hydrology and water quality, but peatland restoration is considered to ameliorate peatland degradation. Using a replicated BACIPS (Before-After-Control-Impact Paired Series) design, we investigated 24 peatlands, all drained for forestry and subsequently restored, and 19 pristine control boreal peatlands with high temporal and spatial resolution data on hydroclimate and pore water quality. In drained conditions, total nitrogen (Ntot), total phosphorus (Ptot), and dissolved organic carbon (DOC) in pore water were several-fold higher than observed at pristine control sites, highlighting the impacts of long-term drainage on pore water quality. In general, pore water DOC and Ntot decreased after restoration measures but still remained significantly higher than at pristine control sites, indicating long time lags in restoration effects. Different peatland classes and trophic levels (vegetation gradient) responded differently to restoration, primarily due to altered hydrology and varying acidity levels. Sites that were hydrologically overrestored (inundated) showed higher Ptot, Ntot, and DOC than well-restored or insufficiently restored sites, indicating the need to optimize natural-like hydrological regimes when restoring peatlands drained for forestry. Rich fens (median pH 6.2-6.6) showed lower pore water Ptot, Ntot, and DOC than intermediate and poor peats (pH 4.0-4.6) both before and after restoration. Nutrients and DOC in pore water increased in the first year postrestoration but decreased thereafter. The most important variables related to pore water quality were trophic level, peatland class, water table level, and soil and air temperature.

  1. Model predictive control for a thermostatic controlled system

    DEFF Research Database (Denmark)

    Shafiei, Seyed Ehsan; Rasmussen, Henrik; Stoustrup, Jakob

    2013-01-01

    This paper proposes a model predictive control scheme to provide temperature set-points to thermostatic controlled cooling units in refrigeration systems. The control problem is formulated as a convex programming problem to minimize the overall operating cost of the system. The foodstuff temperat......This paper proposes a model predictive control scheme to provide temperature set-points to thermostatic controlled cooling units in refrigeration systems. The control problem is formulated as a convex programming problem to minimize the overall operating cost of the system. The foodstuff...

  2. Fuel fabrication instrumentation and control system overview

    International Nuclear Information System (INIS)

    Bennett, D.W.; Fritz, R.L.

    1980-10-01

    A process instrumentation and control system is being developed for automated fabrication of breeder reactor fuel at the Hanford Engineering Development Laboratory (HEDL) in Richland, Washington. The basic elements of the control system are a centralized computer system linked to distributed local computers, which direct individual process applications. The control philosophy developed for the equipment automation program stresses system flexibility and inherent levels of redundant control capabilities. Four different control points have been developed for each unit process operation

  3. Hydraulically powered dissimilar teleoperated system controller design

    International Nuclear Information System (INIS)

    Jansen, J.F.; Kress, R.L.

    1996-01-01

    This paper will address two issues associated with the implementation of a hydraulically powered dissimilar master-slave teleoperated system. These issues are the overall system control architecture and the design of robust hydraulic servo controllers for the position control problem. Finally, a discussion of overall system performance on an actual teleoperated system will be presented

  4. Supervisory Control and Diagnostics System Distributed Operating System

    International Nuclear Information System (INIS)

    McGoldrick, P.R.

    1979-01-01

    This paper contains a description of the Supervisory Control and Diagnostics System (SCDS) Distributed Operating System. The SCDS consists of nine 32-bit minicomputers with shared memory. The system's main purpose is to control a large Mirror Fusion Test Facility

  5. Distributed control system for the FMIT

    International Nuclear Information System (INIS)

    Johnson, J.A.; Machen, D.R.; Suyama, R.M.

    1979-01-01

    The control system for the Fusion Materials Irradiation Test (FMIT) Facility will provide the primary data acquisition, control, and interface components that integrate all of the individual FMIT systems into a functional facility. The control system consists of a distributed computer network, control consoles and instrumentation subsystems. The FMIT Facility will be started, operated and secured from a Central Control Room. All FMIT systems and experimental functions will be monitored from the Central Control Room. The data acquisition and control signals will be handled by a data communications network, which connects dual computers in the Central Control Room to the microcomputers in CAMAC crates near the various subsystems of the facility

  6. Upgrading the Fermilab Linac local control system

    International Nuclear Information System (INIS)

    McCrory, E.S.; Goodwin, R.W.; Shea, M.F.

    1991-02-01

    A new control system for the Fermilab Linac is being designed, built and implemented. First, the nine-year-old linac control system is being replaced. Second, a control system for the new 805 MHz part of the linac is being built. The two systems are essentially identical, so that when the installations are complete, we will still have a single Linac Control System. 8 refs., 5 figs

  7. A Rapid Screen for Host-Encoded miRNAs with Inhibitory Effects against Ebola Virus Using a Transcription- and Replication-Competent Virus-Like Particle System

    Directory of Open Access Journals (Sweden)

    Zhongyi Wang

    2018-05-01

    Full Text Available MicroRNAs (miRNAs may become efficient antiviral agents against the Ebola virus (EBOV targeting viral genomic RNAs or transcripts. We previously conducted a genome-wide search for differentially expressed miRNAs during viral replication and transcription. In this study, we established a rapid screen for miRNAs with inhibitory effects against EBOV using a tetracistronic transcription- and replication-competent virus-like particle (trVLP system. This system uses a minigenome comprising an EBOV leader region, luciferase reporter, VP40, GP, VP24, EBOV trailer region, and three noncoding regions from the EBOV genome and can be used to model the life cycle of EBOV under biosafety level (BSL 2 conditions. Informatic analysis was performed to select up-regulated miRNAs targeting the coding regions of the minigenome with the highest binding energy to perform inhibitory effect screening. Among these miRNAs, miR-150-3p had the most significant inhibitory effect. Reverse transcription polymerase chain reaction (RT-PCR, Western blot, and double fluorescence reporter experiments demonstrated that miR-150-3p inhibited the reproduction of trVLPs via the regulation of GP and VP40 expression by directly targeting the coding regions of GP and VP40. This novel, rapid, and convenient screening method will efficiently facilitate the exploration of miRNAs against EBOV under BSL-2 conditions.

  8. Globalization and localization of Management Control Systems

    DEFF Research Database (Denmark)

    Toldbod, Thomas; Israelsen, Poul

    2014-01-01

    Through an empirical case study this article examines the operation of multiple management control systems as a package in a Danish manufacturing company. The analysis focuses on four different management control systems; cybernetic controls, planning controls, reward controls, and administrative...... have more particular characteristics. Specifically, this study finds that cybernetic controls and administrative controls are designed as global management control systems. Planning controls are glocal systems and reward & compensation controls assume local characteristics. The finding leads...... controls, through the theoretical lens of globalization, localization, and glocalization. The analysis documents that these different management control systems are affected differently by the processes of globalization and localization, whereby some are universal throughout the organization and others...

  9. Enzymatic recognition of DNA replication origins

    International Nuclear Information System (INIS)

    Stayton, M.M.; Bertsch, L.; Biswas, S.

    1983-01-01

    In this paper we discuss the process of recognition of the complementary-strand origin with emphasis on RNA polymerase action in priming M13 DNA replication, the role of primase in G4 DNA replication, and the function of protein n, a priming protein, during primosome assembly. These phage systems do not require several of the bacterial DNA replication enzymes, particularly those involved in the regulation of chromosome copy number of the initiatiion of replication of duplex DNA. 51 references, 13 figures, 1 table

  10. Replicating animal mitochondrial DNA

    Directory of Open Access Journals (Sweden)

    Emily A. McKinney

    2013-01-01

    Full Text Available The field of mitochondrial DNA (mtDNA replication has been experiencing incredible progress in recent years, and yet little is certain about the mechanism(s used by animal cells to replicate this plasmid-like genome. The long-standing strand-displacement model of mammalian mtDNA replication (for which single-stranded DNA intermediates are a hallmark has been intensively challenged by a new set of data, which suggests that replication proceeds via coupled leading-and lagging-strand synthesis (resembling bacterial genome replication and/or via long stretches of RNA intermediates laid on the mtDNA lagging-strand (the so called RITOLS. The set of proteins required for mtDNA replication is small and includes the catalytic and accessory subunits of DNA polymerase y, the mtDNA helicase Twinkle, the mitochondrial single-stranded DNA-binding protein, and the mitochondrial RNA polymerase (which most likely functions as the mtDNA primase. Mutations in the genes coding for the first three proteins are associated with human diseases and premature aging, justifying the research interest in the genetic, biochemical and structural properties of the mtDNA replication machinery. Here we summarize these properties and discuss the current models of mtDNA replication in animal cells.

  11. Who Needs Replication?

    Science.gov (United States)

    Porte, Graeme

    2013-01-01

    In this paper, the editor of a recent Cambridge University Press book on research methods discusses replicating previous key studies to throw more light on their reliability and generalizability. Replication research is presented as an accepted method of validating previous research by providing comparability between the original and replicated…

  12. Control system security in nuclear power plant

    International Nuclear Information System (INIS)

    Li Jianghai; Huang Xiaojin

    2012-01-01

    The digitalization and networking of control systems in nuclear power plants has brought significant improvements in system control, operation and maintenance. However, the highly digitalized control system also introduces additional security vulnerabilities. Moreover, the replacement of conventional proprietary systems with common protocols, software and devices makes these vulnerabilities easy to be exploited. Through the interaction between control systems and the physical world, security issues in control systems impose high risks on health, safety and environment. These security issues may even cause damages of critical infrastructures and threaten national security. The importance of control system security by reviewing several control system security incidents that happened in nuclear power plants was showed in recent years. Several key difficulties in addressing these security issues were described. Finally, existing researches on control system security and propose several promising research directions were reviewed. (authors)

  13. RF control system of the HIMAC synchrotron

    International Nuclear Information System (INIS)

    Kanazawa, M.; Sato, K.; Itano, A.

    1992-01-01

    An RF control system of the HIMAC synchrotron has been constructed. In this control system we have adopted a digital feed back system with a digital synthesizer (DS). Combining a high power system, performance of the control system have been tested in a factory (Toshiba) with a simulator circuit of the synchrotron oscillation. Following this test, We had beam acceleration test with this control system at TARN-II in INS (Institute for Nuclear Study, University of Tokyo). This paper describes the RF control system and its tested results. (author)

  14. Automatic oscillator frequency control system

    Science.gov (United States)

    Smith, S. F. (Inventor)

    1985-01-01

    A frequency control system makes an initial correction of the frequency of its own timing circuit after comparison against a frequency of known accuracy and then sequentially checks and corrects the frequencies of several voltage controlled local oscillator circuits. The timing circuit initiates the machine cycles of a central processing unit which applies a frequency index to an input register in a modulo-sum frequency divider stage and enables a multiplexer to clock an accumulator register in the divider stage with a cyclical signal derived from the oscillator circuit being checked. Upon expiration of the interval, the processing unit compares the remainder held as the contents of the accumulator against a stored zero error constant and applies an appropriate correction word to a correction stage to shift the frequency of the oscillator being checked. A signal from the accumulator register may be used to drive a phase plane ROM and, with periodic shifts in the applied frequency index, to provide frequency shift keying of the resultant output signal. Interposition of a phase adder between the accumulator register and phase plane ROM permits phase shift keying of the output signal by periodic variation in the value of a phase index applied to one input of the phase adder.

  15. Fuzzy logic for structural system control

    Directory of Open Access Journals (Sweden)

    Herbert Martins Gomes

    Full Text Available This paper provides some information and numerical tests that aims to investigate the use of a Fuzzy Controller applied to control systems. Some advantages are reported regarding the use of this controller, such as the characteristic ease of implementation due to its semantic feature in the statement of the control rules. On the other hand, it is also hypothesized that these systems have a lower performance loss when the system to be controlled is nonlinear or has time varying parameters. Numerical tests are performed using modal LQR optimal control and Fuzzy control of non-collocated systems with full state feedback in a two-dimensional structure. The paper proposes a way of designing a controller that may be a supervisory Fuzzy controller for a traditional controller or even a fuzzy controller independent from the traditional control, consisting on individual mode controllers. Some comments are drawn regarding the performance of these proposals in a number of arrangements.

  16. Pressure control of hydraulic servo system using proportional control valve

    International Nuclear Information System (INIS)

    Yang, Kyong Uk; Oh, In Ho; Lee, Ill Yeong

    1999-01-01

    The purpose of this study is to develop a control scheme for the hydraulic servo system which can rapidly control the pressure in a hydraulic cylinder with very short stroke. Compared with the negligible stroke of the cylinder in the system, the flow gain of the proportional pressure control valve constituting the hydraulic servo system is relatively large and the time delay on the response of the valve is quite long. Therefore, the pressure control system, in this study tends to get unstable during operations. Considering the above mentioned characteristics of the system, a two-degree-of-freedom control scheme, composed of the I-PDD 2 ... feedback compensator and the feedforward controller, is proposed. The reference model scheme is used in deciding the parameters of the controllers. The validity of the proposed control scheme is confirmed through the experiments

  17. Noninteracting control of nonlinear systems based on relaxed control

    NARCIS (Netherlands)

    Jayawardhana, B.

    2010-01-01

    In this paper, we propose methodology to solve noninteracting control problem for general nonlinear systems based on the relaxed control technique proposed by Artstein. For a class of nonlinear systems which cannot be stabilized by smooth feedback, a state-feedback relaxed control can be designed to

  18. Constrained control allocation for systems with redundant control effectors

    OpenAIRE

    Bordignon, Kenneth A

    1996-01-01

    Control allocation is examined for linear time-invariant problems that have more controls than degrees of freedom. The controls are part of a physical system and are subject to limits on their maximum positions. A control allocation scheme commands control deflections in response to some desired output. The ability of a control allocation scheme to produce the desired output without violating the physical position constraints is used to compare allocation schemes. Methods are develop...

  19. Seaway Information System Management and Control Requirements

    Science.gov (United States)

    1973-10-01

    This report examines in detail the control and information system requirements of the St. Lawrence Seaway development program in terms of the needs of the vessel traffic controllers and the management users. Structural control models of Seaway operat...

  20. The CEBAF control system for the CHL

    International Nuclear Information System (INIS)

    Keesee, M.S.; Bevins, B.S.

    1996-01-01

    The CEBAF Central Helium Liquefier (CHL) control system consists of independent safety controls located at each subsystem, CAMAC computer interface hardware, and a CEBAF-designed control software called Thaumaturgic Automated Control Logic (TACL). The paper describes how control software was interfaced with the subsystems of the CHL. Topics of configuration, editing, operator interface, datalogging, and internal logic functions are presented as they relate to the operational needs of the helium plant. The paper also describes the effort underway to convert from TACL to the Experimental Physics and Industrial Control System (EPICS), the new control system for the CEBAF accelerator. This software change will require customizing EPICS software to cryogenic process control

  1. NOVANET: communications network for a control system

    International Nuclear Information System (INIS)

    Hill, J.R.; Severyn, J.R.; VanArsdall, P.J.

    1983-01-01

    NOVANET is a control system oriented fiber optic local area network that was designed to meet the unique and often conflicting requirements of the Nova laser control system which will begin operation in 1984. The computers and data acquisition devices that form the distributed control system for a large laser fusion research facility need reliable, high speed communications. Both control/status messages and experimental data must be handled. A subset of NOVANET is currently operating on the two beam Novette laser system

  2. Architecture of the modern accelerator control system

    International Nuclear Information System (INIS)

    Samardzic, B.; Drndarevic, V.

    2000-01-01

    Well defined concept of the system and construction plan are the important conditions for the successful realization of the accelerator control system. In this paper the modern concept of accelerator control system as well as guidelines for its efficient development have been presented. Described concept could be applied for the design of control systems for other types of facilities for experimental physics and for industrial process control. (author)

  3. PLCs for nuclear fire control system

    International Nuclear Information System (INIS)

    McArthur, Neil

    1990-01-01

    The new Thermal Oxide Reprocessing Plant (THORP) at British Nuclear Fuel's Sellafield site is a very large and complex system. This article describes the computerized control system used for fire damage control in the two main production areas, the head end and the chemical separation segments. Over one thousand fire dampers are controlled by an interlinking system of small computers linked to a main system in the central control room. The choice of hardware and software is also described. (UK)

  4. Specification of EDITH motion control system

    International Nuclear Information System (INIS)

    Breitwieser, H.; Frank, A.; Holler, E.; Suess, U.; Leinemann, K.

    1990-09-01

    EDITH is an experimental device for in vessel handling at NET/ITER. The purpose of EDITH is: Testing of ABS (articulated boom system) components; testing and validation of remote handling procedures; testing and validation of ABS end-effectors; testing of ABS control system features and verification of control system concepts. This document, after describing the environment in which the control system is to operate, specifies architecture and functionality to be implemented by the EDITH motion control system software, thereby taking full reference to the control system specification for TARM, which was decided to be the base for the implementation. (orig.) [de

  5. A metamorphic controller for plant control system design

    Directory of Open Access Journals (Sweden)

    Tomasz Klopot

    2016-07-01

    Full Text Available One of the major problems in the design of industrial control systems is the selection and parameterization of the control algorithm. In practice, the most common solution is the PI (proportional-integral controller, which is simple to implement, but is not always the best control strategy. The use of more advanced controllers may result in a better efficiency of the control system. However, the implementation of advanced control algorithms is more time-consuming and requires specialized knowledge from control engineers. To overcome these problems and to support control engineers at the controller design stage, the paper describes a tool, i.e., a metamorphic controller with extended functionality, for selection and implementation of the most suitable control algorithm. In comparison to existing solutions, the main advantage of the metamorphic controller is its possibility of changing the control algorithm. In turn, the candidate algorithms can be tested through simulations and the total time needed to perform all simulations can be less than a few minutes, which is less than or comparable to the design time in the concurrent design approach. Moreover, the use of well-known tuning procedures, makes the system easy to understand and operate even by inexperienced control engineers. The application was implemented in the real industrial programmable logic controller (PLC and tested with linear and nonlinear virtual plants. The obtained simulation results confirm that the change of the control algorithm allows the control objectives to be achieved at lower costs and in less time.

  6. Adaptive control of port-Hamiltonian systems

    NARCIS (Netherlands)

    Dirksz, D.A.; Scherpen, J.M.A.; Edelmayer, András

    2010-01-01

    In this paper an adaptive control scheme is presented for general port-Hamiltonian systems. Adaptive control is used to compensate for control errors that are caused by unknown or uncertain parameter values of a system. The adaptive control is also combined with canonical transformation theory for

  7. Logistics hardware and services control system

    Science.gov (United States)

    Koromilas, A.; Miller, K.; Lamb, T.

    1973-01-01

    Software system permits onsite direct control of logistics operations, which include spare parts, initial installation, tool control, and repairable parts status and control, through all facets of operations. System integrates logistics actions and controls receipts, issues, loans, repairs, fabrications, and modifications and assets in predicting and allocating logistics parts and services effectively.

  8. Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins.

    Science.gov (United States)

    Foulk, Michael S; Urban, John M; Casella, Cinzia; Gerbi, Susan A

    2015-05-01

    Nascent strand sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification of features for their specification. NS-seq depends on the ability of lambda exonuclease (λ-exo) to efficiently digest parental DNA while leaving RNA-primer protected nascent strands intact. We used genomics and biochemical approaches to determine if λ-exo digests all parental DNA sequences equally. We report that λ-exo does not efficiently digest G-quadruplex (G4) structures in a plasmid. Moreover, λ-exo digestion of nonreplicating genomic DNA (LexoG0) enriches GC-rich DNA and G4 motifs genome-wide. We used LexoG0 data to control for nascent strand-independent λ-exo biases in NS-seq and validated this approach at the rDNA locus. The λ-exo-controlled NS-seq peaks are not GC-rich, and only 35.5% overlap with 6.8% of all G4s, suggesting that G4s are not general determinants for origin specification but may play a role for a subset. Interestingly, we observed a periodic spacing of G4 motifs and nucleosomes around the peak summits, suggesting that G4s may position nucleosomes at this subset of origins. Finally, we demonstrate that use of Na(+) instead of K(+) in the λ-exo digestion buffer reduced the effect of G4s on λ-exo digestion and discuss ways to increase both the sensitivity and specificity of NS-seq. © 2015 Foulk et al.; Published by Cold Spring Harbor Laboratory Press.

  9. Technical Area 55 Entry Control System (ECS)

    International Nuclear Information System (INIS)

    Beaumont, A.; Brundige, E.; DesJardin, R.; Rivera, R.

    1984-01-01

    The exchange badge system which was used at the Plutonium Facility located in Technical Area 55 was replaced on a trial basis with an automated Entry Control System. As a result of the success of the trial system, a new system incorporating expanded features and increased reliability is being implemented. The new Entry Control System incorporates several features not previously available in relatively inexpensive entry systems. The reliability of the system is enhanced by redundant microprocessors incorporating bubble memory for nonvolatile storage of the system data base. The badge readers incorporate dual communication lines to two different controllers to further increase the total system reliability

  10. The Optimization of power reactor control system

    International Nuclear Information System (INIS)

    Danupoyo, S.D.

    1997-01-01

    A power reactor is an important part in nuclear powered electrical plant systems. Success in controlling the power reactor will establish safety of the whole power plant systems. Until now, the power reactor has been controlled by a classical control system that was designed based on output feedback method. To meet the safety requirements that are now more restricted, the recently used power reactor control system should be modified. this paper describes a power reactor control system that is designed based on a state feedback method optimized with LQG (Linear-quadrature-gaussian) method and equipped with a state estimator. A pressurized-water type reactor has been used as the model. by using a point kinetics method with one group delayed neutrons. the result of simulation testing shows that the optimized control system can control the power reactor more effective and efficient than the classical control system

  11. Implementation of the DIAC control system

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Dae-Sik; Jang, Doh-Yun; Jin, Jeong-Tae; Oh, Byung-Hoon [KAERI, Daejeon (Korea, Republic of)

    2015-05-15

    DIAC (Daejeon Ion Accelerator Complex) system was developed, and operated at JAEA of Japan by KEK team with a name of TRIAC (Tokai Radioactive Ion Accelerator Complex) during 2004 to 2010. The TRIAC control system was based on LabView and had two independent control units for ion source and accelerator. To be an efficient system, it is necessary to have an integrated control capability. And the control software, which had implemented by using LabView at TRIAC, will be changed with EPICS in order to give an effective beam service to the users. In this presentation, the old TRIAC control system is described, and a new control system for DIAC is discussed. The control system of DIAC is based on TRIAC. But it is gradually improved performance using EPICS toolkits and changing some digital interface hardware of it. Details of the control system will be demonstrated during the conference.

  12. Topography measurements for determining the decay factors in surface replication

    International Nuclear Information System (INIS)

    Song, J; Zheng, A; Vorburger, T V; Rubert, P

    2008-01-01

    The electro-forming technique is used at National Institute of Standards and Technology (NIST) for the production of standard reference material (SRM) 2461 standard casings to support nationwide ballistics measurement traceability and measurement quality control in the US. In order to ensure that the SRM casings are produced with virtually the same surface topography, it is necessary to test the decay factors of the replication process. Twenty-six replica casings are replicated from the same master casing for the decay factor tests. The NIST topography measurement system is used for measurements and correlations of surface topography. The topography decays are quantified by the cross-correlation function maximum CCF max . Based on the test, it is expected that 256 SRM casings can be replicated from the same master with CCF max values higher than 95%

  13. Applying model predictive control to power system frequency control

    OpenAIRE

    Ersdal, AM; Imsland, L; Cecilio, IM; Fabozzi, D; Thornhill, NF

    2013-01-01

    16.07.14 KB Ok to add accepted version to Spiral Model predictive control (MPC) is investigated as a control method which may offer advantages in frequency control of power systems than the control methods applied today, especially in presence of increased renewable energy penetration. The MPC includes constraints on both generation amount and generation rate of change, and it is tested on a one-area system. The proposed MPC is tested against a conventional proportional-integral (PI) cont...

  14. Registered Replication Report

    DEFF Research Database (Denmark)

    Bouwmeester, S.; Verkoeijen, P. P.J.L.; Aczel, B.

    2017-01-01

    and colleagues. The results of studies using time pressure have been mixed, with some replication attempts observing similar patterns (e.g., Rand et al., 2014) and others observing null effects (e.g., Tinghög et al., 2013; Verkoeijen & Bouwmeester, 2014). This Registered Replication Report (RRR) assessed...... the size and variability of the effect of time pressure on cooperative decisions by combining 21 separate, preregistered replications of the critical conditions from Study 7 of the original article (Rand et al., 2012). The primary planned analysis used data from all participants who were randomly assigned...

  15. Status of the ATLAS control system upgrade

    International Nuclear Information System (INIS)

    Munson, F.H.; Ferraretto, M.; Rutherford, B.

    1992-01-01

    Certain components of the ATLAS control system are two generations behind today's technology. It has been decided to upgrade the control system. in part, by replacing Digital Equipment Corporation (DEC) PDP-11 computers with present-day VAX technology. Two primary goals have been defined for the upgraded control system. The first of these goals is to keep additional ''in-house'' written software to a minimum, while providing the portability necessary to ensure the continued use of existing software. In an attempt to achieve this goal, commercially-available software has been utilized to provide a foundation for the final control-system configuration. The second goal is to develop the new control system, while not interfering with accelerator operations. This paper describes some of the motivation for upgrading the ATLAS control system, the basic features of the new control system, and the present status of the system's development

  16. ACHEMA '85: Process control systems

    International Nuclear Information System (INIS)

    Rosskopf, E.

    1985-01-01

    The strategy obviously adopted by the well-established manufacturers is to offer 'easy-to-handle' equipment to gain new customers, and there is a variety of new compact systems or personal computers being put on the market. The changes and improvements within the processing sector proceed more or less in silence; high-capacity storage devices and multiprocessor configurations are obtainable at a moderate price, offering a greater variety of basic functions and enhanced control possibilities. Redundancy problems are handled with greater flexibility, and batch programs are advancing. Data communication has become a common feature, transmission speed and bus length have been improved. Important improvements have been made with regard to data display; even medium-sized equipment now offer the possibility of making dynamic flow-sheets and reserving space for process history display, and the hierarchy of displays has been considerably simplified. The user software also has been made more easy, 'fill-in-the-blancs' is the prevailing motto for dialog configurations, and such big terms as process computer' or 'programming skill' are passing into oblivion. (orig./HP) [de

  17. A CAMAC based knob controller for the LAMPF control system

    International Nuclear Information System (INIS)

    Smith, W.; Bjorklund, E.

    1987-01-01

    The control computer for the Los Alamos Meson Physics Facility (LAMPF) has been recently upgraded from an SEL-840 to a VAX 11/780 running the VMS operating system. As part of this upgrade, a CAMAC-based knob controller was developed for the new control system. The knobs allow the facility operators to have slew control over software selectable accelerator devices. An alphanumeric display associated with each knob monitors the progress of the selected device. This paper describes the system requirements for the new LAMPF knob controller, and the resulting hardware and software design

  18. DNA is a co-factor for its own replication in Xenopus egg extracts

    NARCIS (Netherlands)

    Lebofsky, Ronald; van Oijen, Antoine M.; Walter, Johannes C.

    Soluble Xenopus egg extracts efficiently replicate added plasmids using a physiological mechanism, and thus represent a powerful system to understand vertebrate DNA replication. Surprisingly, DNA replication in this system is highly sensitive to plasmid concentration, being undetectable below

  19. The convergent LEP and SPS control systems

    International Nuclear Information System (INIS)

    Altaber, J.

    1987-01-01

    The major design contraint of the control system for LEP is the compatibility with the existing SPS control system. The first reason for this compatibility is to allow a long term convergence of the SPS control system towards the LEP one. The second reason is to operate both LEP and SPS machines from a unique main control room. The distributed architecture of LEP and the existing SPS control systems are described. The design of the equipment interface for both machines is explained. Finally, the infrastructure of the common main control room for LEP and SPS is described

  20. Fuzzy logic control and optimization system

    Science.gov (United States)

    Lou, Xinsheng [West Hartford, CT

    2012-04-17

    A control system (300) for optimizing a power plant includes a chemical loop having an input for receiving an input signal (369) and an output for outputting an output signal (367), and a hierarchical fuzzy control system (400) operably connected to the chemical loop. The hierarchical fuzzy control system (400) includes a plurality of fuzzy controllers (330). The hierarchical fuzzy control system (400) receives the output signal (367), optimizes the input signal (369) based on the received output signal (367), and outputs an optimized input signal (369) to the input of the chemical loop to control a process of the chemical loop in an optimized manner.

  1. Spacecraft command and control using expert systems

    Science.gov (United States)

    Norcross, Scott; Grieser, William H.

    1994-01-01

    This paper describes a product called the Intelligent Mission Toolkit (IMT), which was created to meet the changing demands of the spacecraft command and control market. IMT is a command and control system built upon an expert system. Its primary functions are to send commands to the spacecraft and process telemetry data received from the spacecraft. It also controls the ground equipment used to support the system, such as encryption gear, and telemetry front-end equipment. Add-on modules allow IMT to control antennas and antenna interface equipment. The design philosophy for IMT is to utilize available commercial products wherever possible. IMT utilizes Gensym's G2 Real-time Expert System as the core of the system. G2 is responsible for overall system control, spacecraft commanding control, and spacecraft telemetry analysis and display. Other commercial products incorporated into IMT include the SYBASE relational database management system and Loral Test and Integration Systems' System 500 for telemetry front-end processing.

  2. Automatic synthesis of supervisory control systems

    NARCIS (Netherlands)

    Najafi, E.

    2016-01-01

    Sequential composition is an effective supervisory control method for addressing control problems in nonlinear dynamical systems. It executes a set of controllers sequentially to achieve a control specification that cannot be realized by a single controller. Sequential composition focuses on the

  3. Towards Autonomous Control of Hydraulic Actuator Systems

    DEFF Research Database (Denmark)

    Hansen, Poul Erik; Conrad, Finn

    1998-01-01

    Presentation of new developed control algorithms to increase autonomy and intelligence of hydraulic control systems. A refinement of relaytuning method is used to determine the control parameters of a lag/lead controller and a poleplacement controller. Further, a fail-safe function is developed...

  4. The replication recipe : What makes for a convincing replication?

    NARCIS (Netherlands)

    Brandt, M.J.; IJzerman, H.; Dijksterhuis, Ap; Farach, Frank J.; Geller, Jason; Giner-Sorolla, Roger; Grange, James A.; Perugini, Marco; Spies, Jeffrey R.; van 't Veer, Anna

    Psychological scientists have recently started to reconsider the importance of close replications in building a cumulative knowledge base; however, there is no consensus about what constitutes a convincing close replication study. To facilitate convincing close replication attempts we have developed

  5. The Replication Recipe: What makes for a convincing replication?

    NARCIS (Netherlands)

    Brandt, M.J.; IJzerman, H.; Dijksterhuis, A.J.; Farach, F.J.; Geller, J.; Giner-Sorolla, R.; Grange, J.A.; Perugini, M.; Spies, J.R.; Veer, A. van 't

    2014-01-01

    Psychological scientists have recently started to reconsider the importance of close replications in building a cumulative knowledge base; however, there is no consensus about what constitutes a convincing close replication study. To facilitate convincing close replication attempts we have developed

  6. Control of the Tevatron Satellite Refrigeration system

    International Nuclear Information System (INIS)

    Theilacker, J.; Chapman, L.; Gannon, J.; Hentges, M.; Martin, M.; Rode, C.H.; Zagel, J.

    1984-01-01

    This chapter describes a computerized control system for 24 satellite refrigerators which cool a six kilometer ring of superconducting magnets. The control system consists of 31 independent microprocessors operating over 400 servo loops, and a central computer system which provides monitoring, alarms, logging and changing of parameters. Topics considered include pressure measurement, flow measurement, temperature measurement, gas analysis, control valves, expansion engine controllers, and control loops. Each refrigerator has 12 active microprocessor based control loops which tune the refrigerator to one of its four operating modes: satellite, liquefier, refrigerator, and stand-by. It is suggested that optimizing the refrigerator control loops and quench recovery scheme will minimize the accelerator down time

  7. Integrated control system for electron beam processes

    Science.gov (United States)

    Koleva, L.; Koleva, E.; Batchkova, I.; Mladenov, G.

    2018-03-01

    The ISO/IEC 62264 standard is widely used for integration of the business systems of a manufacturer with the corresponding manufacturing control systems based on hierarchical equipment models, functional data and manufacturing operations activity models. In order to achieve the integration of control systems, formal object communication models must be developed, together with manufacturing operations activity models, which coordinate the integration between different levels of control. In this article, the development of integrated control system for electron beam welding process is presented as part of a fully integrated control system of an electron beam plant, including also other additional processes: surface modification, electron beam evaporation, selective melting and electron beam diagnostics.

  8. Relaxation in control systems of subdifferential type

    Science.gov (United States)

    Tolstonogov, A. A.

    2006-02-01

    In a separable Hilbert space we consider a control system with evolution operators that are subdifferentials of a proper convex lower semicontinuous function depending on time. The constraint on the control is given by a multivalued function with non-convex values that is lower semicontinuous with respect to the variable states. Along with the original system we consider the system in which the constraint on the control is the upper semicontinuous convex-valued regularization of the original constraint. We study relations between the solution sets of these systems. As an application we consider a control variational inequality. We give an example of a control system of parabolic type with an obstacle.

  9. Relaxation in control systems of subdifferential type

    International Nuclear Information System (INIS)

    Tolstonogov, A A

    2006-01-01

    In a separable Hilbert space we consider a control system with evolution operators that are subdifferentials of a proper convex lower semicontinuous function depending on time. The constraint on the control is given by a multivalued function with non-convex values that is lower semicontinuous with respect to the variable states. Along with the original system we consider the system in which the constraint on the control is the upper semicontinuous convex-valued regularization of the original constraint. We study relations between the solution sets of these systems. As an application we consider a control variational inequality. We give an example of a control system of parabolic type with an obstacle

  10. REACTOR CONTROL ROD OPERATING SYSTEM

    Science.gov (United States)

    Miller, G.

    1961-12-12

    A nuclear reactor control rod mechanism is designed which mechanically moves the control rods into and out of the core under normal conditions but rapidly forces the control rods into the core by catapultic action in the event of an emergency. (AEC)

  11. Control Systems and Number Theory

    Directory of Open Access Journals (Sweden)

    Fuhuo Li

    2012-01-01

    and PID-controllers are applied successfully in the EV control by J.-Y. Cao and B.-G. Cao 2006 and Cao et al. 2007, which we may unify in our framework. Finally, we mention some similarities between control theory and zeta-functions.

  12. Design of embedded control systems

    National Research Council Canada - National Science Library

    Adamski, M; Karatkevich, Andrei; Węgrzyn, M

    2005-01-01

    ... using some concrete technological base; efficient methods of logic synthesis, test, and verification should be developed for that. Fourth, the task of the communication between the control device and controlled objects (and maybe between different control devices) waits for its solution. All these problems are hard enough and cannot be successfull...

  13. Control and estimation of piecewise affine systems

    CERN Document Server

    Xu, Jun

    2014-01-01

    As a powerful tool to study nonlinear systems and hybrid systems, piecewise affine (PWA) systems have been widely applied to mechanical systems. Control and Estimation of Piecewise Affine Systems presents several research findings relating to the control and estimation of PWA systems in one unified view. Chapters in this title discuss stability results of PWA systems, using piecewise quadratic Lyapunov functions and piecewise homogeneous polynomial Lyapunov functions. Explicit necessary and sufficient conditions for the controllability and reachability of a class of PWA systems are

  14. Safety-related control air systems

    International Nuclear Information System (INIS)

    Anon.

    1977-01-01

    This Standard applies to those portions of the control air system that furnish air required to support, control, or operate systems or portions of systems that are safety related in nuclear power plants. This Standard relates only to the air supply system(s) for safety-related air operated devices and does not apply to the safety-related air operated device or to air operated actuators for such devices. The objectives of this Standard are to provide (1) minimum system design requirements for equipment, piping, instruments, controls, and wiring that constitute the air supply system; and (2) the system and component testing and maintenance requirements

  15. The rejuvenation of TRISTAN control system

    International Nuclear Information System (INIS)

    Mimashi, T.; Urakawa, J.; Kurokawa, S.; Kawamoto, T.; Takeda, S.; Akiyama, A.; Kudoh, K.; Komada, K.; Naitoh, T.

    1992-01-01

    The current TRISTAN accelerator control system uses CAMAC as a front end electronics, and they are controlled by twenty five Hitachi minicomputer HIDIC 80's which are linked with an N-to-N token ring network. After five years from now, these computers must be replaced. This is because of the life time of control system and we have to cope with the requirements imposed by our future project such as the KEK B-Factory and the main ring photon factory projects. The rejuvenation of this control has to be done under some constraints such as the lack of manpower, limited time and financing. First we review the problems of current control system, then the philosophy of the new generation control system is presented. Finally it is discussed how to move to the new generation control system from the current TRISTAN control system. (author)

  16. Design of a distributed control system

    Energy Technology Data Exchange (ETDEWEB)

    Bilous, O [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1959-07-01

    A digital computer is used to evaluate various pressure control systems for a gaseous diffusion cascade. This is an example of a distributed feedback control system. The paper gives a brief discussion of similar cases of distributed or stage wise control systems, which may occur in multiple temperature control of chemical processes. (author) [French] Une calculatrice digitale est utilisee pour evaluer divers systemes de controle de pression pour une cascade de diffusion gazeuse. C'est un exemple de systeme de controle a reaction distribue. Le rapport presente une breve discussion de cas semblables de systemes de controle distribues ou en etage, qui peuvent se presenter dans de nombreux controles de temperature de reactions chimiques. (auteur)

  17. The cryogenic control system of BEPCⅡ

    Institute of Scientific and Technical Information of China (English)

    LI Gang; WANG Ke-Xiang; ZHAO Ji-Jiu; YUE Ke-Juan; DAI Ming-Sui; HUANG Yi-Ling; JIANG Bo

    2008-01-01

    A superconducting cryogenic system has been designed and deployed in the Beijing Electron-Positron Collider Upgrade Project(BEPCⅡ).The system consists of a Siemens PLC(ST-PLC,Programmable Logic Controller)for the compressor control,an Allen Bradley(AB)PLC for the cryogenic equipments,and the Experimental Physics and Industrial Control System(EPICS)that integrates the PLCs.The system fully automates the superconducting cryogenic control with process control,PID(Proportional-Integral-Differential)control loops,real-time data access and data storage,alarm handler and human machine interface.It is capable of automatic recovery as well.This paper describes the BEPCⅡ cryogenic control system,data communication between ST-PLC and EPICS Input/Output Controllers(IOCs),and the integration of the flow control,the low level interlock,the AB-PLC,and EPICS.

  18. Supervisory control system implemented in programmable logical controller web server

    OpenAIRE

    Milavec, Simon

    2012-01-01

    In this thesis, we study the feasibility of supervisory control and data acquisition (SCADA) system realisation in a web server of a programmable logic controller. With the introduction of Ethernet protocol to the area of process control, the more powerful programmable logic controllers obtained integrated web servers. The web server of a programmable logic controller, produced by Siemens, will also be described in this thesis. Firstly, the software and the hardware equipment used for real...

  19. Eukaryotic DNA Replication Fork.

    Science.gov (United States)

    Burgers, Peter M J; Kunkel, Thomas A

    2017-06-20

    This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed. The preponderance of evidence supports a model in which DNA polymerase ε (Pol ε) carries out the bulk of leading strand DNA synthesis at an undisturbed replication fork. DNA polymerases α and δ carry out the initiation of Okazaki fragment synthesis and its elongation and maturation, respectively. This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork.

  20. Modeling DNA Replication.

    Science.gov (United States)

    Bennett, Joan

    1998-01-01

    Recommends the use of a model of DNA made out of Velcro to help students visualize the steps of DNA replication. Includes a materials list, construction directions, and details of the demonstration using the model parts. (DDR)