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Sample records for repetitive rep eric

  1. Differentiation of banding patterns between Streptococcus mutans and Streptococcus sobrinus isolates in rep-PCR using ERIC primer

    Science.gov (United States)

    Okada, Tamami; Takada, Kazuko; Fujita, Kou; Ikemi, Takuji; Osgood, Robert C.; Childers, Noel K.; Michalek, Suzanne M.

    2011-01-01

    Background Streptococcus mutans and Streptococcus sobrinus are considered to be important bacterial species in the initiation of human dental caries. Therefore, the establishment of a reliable genotyping method to distinguish S. mutans from S. sobrinus is of central importance. Objective We assessed the usefulness of repetitive extragenic palindromic polymerase chain reaction (rep-PCR) using ERIC primer banding patterns in differentiating S. mutans and S. sobrinus. Design Five S. mutans and two S. sobrinus prototype strains and 50 clinical isolates (38 S. mutans serotype c, 4 S. sobrinus serotype d, and 8 S. sobrinus serotype g) were examined. The banding patterns of amplicons generated were compared among the prototype strains and clinical isolates, to find common bands that distinguish S. mutans and S. sobrinus. Results Multiple banding patterns were seen with all strains tested. The representative strains of S. mutans tested revealed six unique, strong bands at 2,000 bp, 1,700 bp, 1,400 bp, 1,100 bp, 850 bp, and 250 bp, whereas S. sobrinus had seven strong bands at 2,000 bp, 1,800 bp, 1,100 bp, 900 bp, 800 bp, 600 bp, and 550 bp. The band at 1,100 bp was the only band that was observed in both S. mutans and S. sobrinus. Furthermore, most clinical S. mutans isolates revealed identical banding patterns. All S. mutans had amplicons at 1,700 bp, 850 bp, and 250 bp, whereas those of S. sobrinus were at 1,100 bp, 900 bp, and 800 bp. Conclusions These results indicate that using rep-PCR with the ERIC primers can distinguish between S. mutans and S. sobrinus. PMID:22145074

  2. ERIC and REP-PCR banding patterns and sequence analysis of the internal transcribed spacer of rDNA of Stemphylium solani isolates from cotton.

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    Mehta, Yeshwant R; Mehta, Angela; Rosato, Yoko B

    2002-05-01

    The genetic diversity of the Stemphylium solani isolates from cotton was assessed by Enterobacterial Repetitive Intergenic Consensus (ERIC) and Repetitive Extragenic Palindromes (REP)-PCR fingerprinting. Twenty eight monosporic isolates of S. solani from cotton were used along with five isolates from tomato and one isolate of Alternaria macrospora from cotton for comparison. The dendrogram obtained revealed clear differences between the cotton and tomato isolates as well as between the tomato isolates from different geographic regions. The genetic relationships among S. solani isolates were also analyzed by sequencing the internal transcribed spacer (ITS) region of four isolates representing the three ERIC and REP groups. The tomato isolate from the State of São Paulo showed a distinct ITS sequence from that of the cotton isolates and tomato isolate from the State of Goiás, giving evidence that it belongs to a different genotype of S. solani. This is the first report of the entire sequence of the ITS1-5.8S-ITS2 regions of S. solani.

  3. Mycoplasma pneumoniae large DNA repetitive elements RepMP1 show type specific organization among strains.

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    Oxana Musatovova

    Full Text Available Mycoplasma pneumoniae is the smallest self-replicating bacterium with a streamlined genome of 0.81 Mb. Complete genome analysis revealed the presence of multiple copies of four large repetitive elements (designated RepMP1, RepMP2/3, RepMP4 and RepMP5 that are implicated in creating sequence variations among individual strains. Recently, we described RepMP1-associated sequence variations between reference strain M129 and clinical isolate S1 that involved three RepMP1-genes (i.e. mpn130, mpn137 and mpn138. Using PCR and sequencing we analyze 28 additional M. pneumoniae strains and demonstrate the existence of S1-like sequence variants in nine strains and M129-like variants in the remaining nineteen strains. We propose a series of recombination steps that facilitates transition from M129- to S1-like sequence variants. Next we examined the remaining RepMP1-genes and observed no other rearrangements related to the repeat element. The only other detected difference was varying numbers of the 21-nucleotide tandem repeats within mpn127, mpn137, mpn501 and mpn524. Furthermore, typing of strains through analysis of large RepMPs localized within the adhesin P1 operon revealed that sequence divergence involving RepMP1-genes mpn130, mpn137 and mpn138 is strictly type-specific. Once more our analysis confirmed existence of two highly conserved groups of M. pneumoniae strains.

  4. Variation in extragenic repetitive DNA sequences in Pseudomonas syringae and potential use of modified REP primers in the identification of closely related isolates

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    Elif Çepni

    2012-01-01

    Full Text Available In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymorphism in the banding patterns. Binding of the primers to different annealing sites in the genome revealed additional fingerprint patterns in eight isolates of P. savastanoi pv. savastanoi and two isolates of P. syringae pv. tomato. The use of four different bases in the primer sequences did not affect the PCR reproducibility and was very efficient in revealing intra-pathovar diversity, particularly in P. savastanoi pv. savastanoi. At the pathovar level, the primer BOX1AR yielded shared fragments, in addition to five bands that discriminated among the pathovars P. syringae pv. phaseolicola, P. savastanoi pv. savastanoi and P. syringae pv. tomato. REP-PCR with a modified primer containing C produced identical bands among the isolates in a pathovar but separated three pathovars more distinctly than four other primers. Although REP-and BOX-PCRs have been successfully used in the molecular identification of Pseudomonas isolates from Turkish flora, a PCR based on inter-enterobacterial repetitive intergenic concensus (ERIC sequences failed to produce clear banding patterns in this study.

  5. RepPop: a database for repetitive elements in Populus trichocarpa

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    Xu Ying

    2009-01-01

    Full Text Available Abstract Background Populus trichocarpa is the first tree genome to be completed, and its whole genome is currently being assembled. No functional annotation about the repetitive elements in the Populus trichocarpa genome is currently available. Results We predicted 9,623 repetitive elements in the Populus trichocarpa genome, and assigned functions to 3,075 of them (31.95%. The 9,623 repetitive elements cover ~40% of the current (partially assembled genome. Among the 9,623 repetitive elements, 668 have copies only in the contigs that have not been assigned to one of the 19 chromosome while the rest all have copies in the partially assembled chromosomes. Conclusion All the predicted data are organized into an easy-to-use web-browsable database, RepPop. Various search capabilities are provided against the RepPop database. A Wiki system has been set up to facilitate functional annotation and curation of the repetitive elements by a community rather than just the database developer. The database RepPop will facilitate the assembling and functional characterization of the Populus trichocarpa genome.

  6. ReRep: Computational detection of repetitive sequences in genome survey sequences (GSS

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    Alves-Ferreira Marcelo

    2008-09-01

    Full Text Available Abstract Background Genome survey sequences (GSS offer a preliminary global view of a genome since, unlike ESTs, they cover coding as well as non-coding DNA and include repetitive regions of the genome. A more precise estimation of the nature, quantity and variability of repetitive sequences very early in a genome sequencing project is of considerable importance, as such data strongly influence the estimation of genome coverage, library quality and progress in scaffold construction. Also, the elimination of repetitive sequences from the initial assembly process is important to avoid errors and unnecessary complexity. Repetitive sequences are also of interest in a variety of other studies, for instance as molecular markers. Results We designed and implemented a straightforward pipeline called ReRep, which combines bioinformatics tools for identifying repetitive structures in a GSS dataset. In a case study, we first applied the pipeline to a set of 970 GSSs, sequenced in our laboratory from the human pathogen Leishmania braziliensis, the causative agent of leishmaniosis, an important public health problem in Brazil. We also verified the applicability of ReRep to new sequencing technologies using a set of 454-reads of an Escheria coli. The behaviour of several parameters in the algorithm is evaluated and suggestions are made for tuning of the analysis. Conclusion The ReRep approach for identification of repetitive elements in GSS datasets proved to be straightforward and efficient. Several potential repetitive sequences were found in a L. braziliensis GSS dataset generated in our laboratory, and further validated by the analysis of a more complete genomic dataset from the EMBL and Sanger Centre databases. ReRep also identified most of the E. coli K12 repeats prior to assembly in an example dataset obtained by automated sequencing using 454 technology. The parameters controlling the algorithm behaved consistently and may be tuned to the properties

  7. Molecular typing among beef isolates of Escherichia coli using consensus repetitive intergenic enterobacteria-polymerase chain reaction (ERIC-PCR)

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    Zoolkifli, Nurliyana Wan; Mutalib, Sahilah Abd

    2013-11-01

    Genomic DNA of Escherichia coli were characterized by enterobacterial repetitive intergenic consensus-Polymerase chain reaction (ERIC-PCR) and the presence of Shiga toxin gene-I (Stx1) and Shiga toxin gene-2 (Stx2). These isolates were originated from imported raw beef which are come from two countries namely Australia and India. The isolation of E. coli was conducted by using Eosin Methylene Blue Agar (EMBA). A total of 94 strains had been isolated from 30 samples of imported raw beefand 42 strains had been detected positively E. coli by doing biochemical tests. All strains had been tested and the results of biochemical tests showed that 3 strains were from Australia samples while the other 39 strains were from India samples. The biochemical tests used are Indole test, Methyl Red test, Voges-Proskauer test and Citrate test. All the 42 strains were examined for Shiga toxin (stx1 and stx2) gene detection by two pair primers which are stx2F (5'-TTCTTCGGTATCCTATTCCC-3'), stx2R (5'-ATGCATCTCTGGTCATTGTA-3'), stx1F (5'-CAGTTAATGTGGTGGCGAAG-3'), and stx1R (5'-CTGTCACAGTAACAACCGT-3'). The results showed that none of the strains are positive for Shiga toxin gene. Application of ERIC-PCR method towards E. coli had successfully shown the high diversity polymorphism in 21 different genome types of DNA with primers ERIC1R (5'- CACTTAGGGGTCCTCGAATGTA- 3') and ERIC2R (5'- AAGTAAGTGACTGGGGTGACGC- 3').

  8. Discriminação de sorovares de Salmonella spp. isolados de carcaças de frango por REP e ERIC-PCR e fagotipagem do sorovar Enteriditis Discrimination of Salmonella serovars isolated from chicken meat by REP and ERIC-PCR and phagotyping of Enteriditis sorovar

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    Iliana Alcocer

    2006-06-01

    Full Text Available Salmonelose é a infecção bacteriana de origem alimentar mais freqüente no Paraná, Brasil, e os surtos estão associados, principalmente, ao consumo de ovos, carne de aves e derivados. Os objetivos deste trabalho foram identificar os sorovares de Salmonella isolados de carcaças de frango e caracterizá-los molecularmente por REP e ERIC-PCR, assim como identificar os fagotipos de Salmonella Enteriditis. Dos 25 isolados de Salmonella spp. analisados, 18 foram identificados como Enteriditis, 4 como Braenderup, 2 como Worthington e 1 como infantis. Dos 18 isolados de Enteriditis, 14 foram PT4, 2 PT4a, 1 PT7 e 1 RDNC, por se tratar de colônia rugosa. REP-PCR forneceu padrão eletroforético distinto de 10 a 13 bandas distribuídas entre 120 e 2072 pb para cada sorovar diferente testado. A ERIC-PCR mostrou um padrão de 4 a 5 bandas entre 180 e 1000 pb e foi menos discriminativa quando comparada à REP-PCR. Os resultados encontrados confirmaram que a fagotipagem é uma ferramenta útil e discriminativa para o sorovar Enteriditis. Apesar do pequeno número de sorovares testados, os resultados sugerem que a REP-PCR parece ser um método atrativo a ser utilizado no futuro para a discriminação preliminar de sorovares de Salmonella.Salmonellosis is the most prevalent bacterial food-borne disease in the State of Paraná, Brazil, and the outbreaks are often associated with consumption of poultry products. The aim of this study was to serotype Salmonella strains isolated from chicken carcasses and characterize them molecularly using REP and ERIC-PCR. The phage types of Salmonella Enteriditis were also identified. Of the 25 Salmonella strains analysed, 18 were identified as Enteriditis, 4 as Braenderup, 2 as Worthington and 1 as infantis. Of the 18 Enteriditis isolates, 14 were PT4, 2 PT4a, 1 PT7 and 1 "reacted, but did not conform" - RDNC. Distinct REP-PCR profiles with 10 to 13 fragments distributed between 120 and 2072 pb were easily obtained for

  9. Identification of new isolates of Bacillus thuringiensis using rep-PCR products and d-endotoxin electron microscopy

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    Lima A.S.G.

    2002-01-01

    Full Text Available PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic and ERIC (Enterobacterial Repetitive Intergenic Consensus sequences (rep-PCR found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.

  10. Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy

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    A.S.G. Lima

    2002-01-01

    Full Text Available PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic and ERIC (Enterobacterial Repetitive Intergenic Consensus sequences (rep-PCR found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.

  11. Repetitive sequence based polymerase chain reaction to differentiate close bacteria strains in acidic sites

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    XIE Ming; YIN Hua-qun; LIU Yi; LIU Jie; LIU Xue-duan

    2008-01-01

    To study the diversity of bacteria strains newly isolated from several acid mine drainage(AMD) sites in China,repetitive sequence based polymerase chain reaction (rep-PCR),a well established technology for diversity analysis of closely related bacteria strains,was conducted on 30 strains of bacteria Leptospirillum ferriphilium,8 strains of bacteria Acidithiobacillus ferrooxidans,as well as the Acidithiobacillus ferrooxidans type strain ATCC (American Type Culture Collection) 23270.The results showed that,using ERIC and BOX primer sets,rep-PCR produced highly discriminatory banding patterns.Phylogenetic analysis based on ERIC-PCR banding types was made and the results indicated that rep-PCR could be used as a rapid and highly discriminatory screening technique in studying bacterial diversity,especially in differentiating bacteria within one species in AMD.

  12. rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis.

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    Louws, F J; Bell, J; Medina-Mora, C M; Smart, C D; Opgenorth, D; Ishimaru, C A; Hausbeck, M K; de Bruijn, F J; Fulbright, D W

    1998-08-01

    ABSTRACT The genomic DNA fingerprinting technique known as repetitive-sequence-based polymerase chain reaction (rep-PCR) was evaluated as a tool to differentiate subspecies of Clavibacter michiganensis, with special emphasis on C. michiganensis subsp. michiganensis, the pathogen responsible for bacterial canker of tomato. DNA primers (REP, ERIC, and BOX), corresponding to conserved repetitive element motifs in the genomes of diverse bacterial species, were used to generate genomic fingerprints of C. michiganensis subsp. michiganensis, C. michiganensis subsp. sepedonicus, C. michiganensis subsp. nebraskensis, C. michiganensis subsp. tessellarius, and C. michiganensis subsp. insidiosum. The rep-PCR-generated patterns of DNA fragments observed after agarose gel electrophoresis support the current division of C. michiganensis into five subspecies. In addition, the rep-PCR fingerprints identified at least four types (A, B, C, and D) within C. michiganensis subsp. michiganensis based on limited DNA polymorphisms; the ability to differentiate individual strains may be of potential use in studies on the epidemiology and host-pathogen interactions of this organism. In addition, we have recovered from diseased tomato plants a relatively large number of naturally occurring avirulent C. michiganensis subsp. michiganensis strains with rep-PCR fingerprints identical to those of virulent C. michiganensis subsp. michiganensis strains.

  13. Multi-Locus Sequence Typing (MLST) and Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR), characterization of shigella spp. over two decades in Tianjin China

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    Cao, Yang; Wei, Dianjun; kamara, Idrissa L; Chen, Wei

    2012-01-01

    To understand the change of the dominant serogroup of Shigella spp., their antimicrobial resistance over more than two decades in Tianjin, their phylogenetic similarity and to determine their evolutionary biology by using REP-PCR and MLST in order to study their epidemiological character. Multi-locus Sequence Typing was performed to determine their lineage and phylogenetic similarity. REP-PCR typing was used to study the homology of their genomic DNA. The isolated rate of group D Shigella in 2009 and 2010 had obviously increased. Antimicrobial susceptibility test results showed that the resistant rates of the 1981-1983 Shigella flexneri to tetracycline, streptomycin and chloramphenicol varied from 76.47 to 100%, they were all sensitive to other antibiotics. During 2009-2010, the resistance rates of the isolated Shigella flexneri to gentamicin, amikacin, third and fourth Generation Cephalosporins and quinolones had increased. MLST results produced five sequence types and two sequence type complexes. REP-PCR showed DNA band similarities between the 1981-1983 and 2009-2010 strains. The dominant serogroup of Shigella in Tianjin has changed from Shigella flexneri to Shigella sonnei. Increased drug resistance of Shigella flexneri is higher than Shigella sonnei because a great variety of antibiotics has been used. The MLST results showed that the 1981-1983 strains had the same sequence type with some of the 2009-2010 strains. Combination of MLST and REP-PCR produced better discriminatory power than using either method alone. PMID:23205184

  14. ERIC-PCR技术对单增李斯特菌的溯源分析%Biotracing the source of Listeria monocytogenes strains by enterobacterial repetitive intergenic consensus sequence-based PCR

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    刘海泉; 朱颖; 姜文洁; 孙晓红; 吴启华; 潘迎捷; 赵勇

    2013-01-01

    Enterobacterial repetitive intergenic consensus sequence (ERIC )-PCR was used to genotype 17 strains of Listeria monocytogenes,which were isolated from pork samples of the three market,and we investigated the correlation between the genotype,regional distribution and prevalence among L monocytogenes strains. L monocytogenes ATCC 19115 was used as positive control. The result showed that 17 isolates were identified as six special genotypes,and genotype IV was the dominant one as the main pollution group,which were isolated from the third market. The strains isolated from the first and second market were genotype I and genotype IV .respcetively. The result suggested that ERIC-PCR was suitable to investigate the biotracing of L. monocytogenes and it was a more rapid,efficient,and accurate molecular typing method than traditional serotyping methods.%以质控菌株ATCC 19115为对照,采用ERIC-PCR方法对从三个市场猪肉样品分离到的17株单增李斯特菌(Listeria monocytogenes)进行了基因分型,探讨了单增李斯特菌基因型与区域分布及流行性的关联性.结果表明,17株单增李斯特菌菌株可分为六个主要基因类群,其中Ⅳ型菌株最多,为主要污染类群,而这些菌株来自于市场三;市场一和市场二分离到的菌株主要分别为Ⅰ型和Ⅳ型.因此,ERIC-PCR方法适用于对单增李斯特菌的溯源分析和流行病学调查,具有简单、方便、快捷、准确的特点.

  15. Genetic Diversity of Food-Isolated Salmonella Strains through Pulsed Field Gel Electrophoresis (PFGE) and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)

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    Fendri, Imen; Ben Hassena, Amal; Grosset, Noel; Barkallah, Mohamed; Khannous, Lamia; Chuat, Victoria; Gautier, Michel; Gdoura, Radhouane

    2013-01-01

    All over the world, the incidence of Salmonella spp contamination on different food sources like broilers, clams and cow milk has increased rapidly in recent years. The multifaceted properties of Salomnella serovars allow the microorganism to grow and multiply in various food matrices, even under adverse conditions. Therefore, methods are needed to detect and trace this pathogen along the entire food supply network. In the present work, PFGE and ERIC-PCR were used to subtype 45 Salmonella isolates belonging to different serovars and derived from different food origins. Among these isolates, S. Enteritidis and S. Kentucky were found to be the most predominant serovars. The Discrimination Index obtained by ERIC-PCR (0.85) was slightly below the acceptable confidence value. The best discriminatory ability was observed when PFGE typing method was used alone (DI = 0.94) or combined with ERIC-PCR (DI = 0.93). A wide variety of profiles was observed between the different serovars using PFGE or/and ERIC-PCR. This diversity is particularly important when the sample origins are varied and even within the same sampling origin. PMID:24312546

  16. Genetic typing of vibrio parahaemolyticus with enterobacterial repetitive intergenic consensus (ERIC) sequences%副溶血性弧菌肠道细菌基因间重复序列基因分型研究

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    宋启发; 叶硕; 徐景野; 章丹阳

    2013-01-01

    目的 检测副溶血性弧菌热稳定溶血素(TDH)基因(tdh),并采用肠道细菌基因间重复序列(Enterobacterial Repetitive Intergenic Consensus-PCR,ERIC-PCR)基因分型技术对tdh阳性和阴性菌株进行聚类分析.方法 PCR法扩增79株分离自患者和海产品中的副溶血性弧菌tdh基因.ERIC-PCR分型PCR产物中某一特定大小片段存在标记为1,否则标记为0,形成二进制独特矩阵,定义为一个基因型,用聚类分析软件NTsys 2.10e对所有菌株、tdh阳性组和阴性组进行聚类分析.结果 患者组、海产品组中分离菌株tdh阳性率分别为87.8% (43/49)和3.3% (1/30),患者中所分离的副溶血性弧菌tdh阳性率明显高于环境中所分离的(x2=19.11,P<0.01).79例菌株可获得产物长度160 bp、360 bp、420 bp、500 bp、680 bp、800 bp、950 bp、1 100 bp、1 350 bp、1 550 bp、1 800 bp、2 100 bp和2400 bp共13种大小不同的PCR产物片段,所有菌株根据扩增片段分布可分为17类,有3个明显族.结论 ERIC-PCR聚类分析结果表明,我国分离的副溶血性弧菌具有较高基因多态性,tdh阴性组离散度高于tdh阳性组.

  17. Determination of genetic differences between fluid and nonfluid variants of Clavibacter michiganensis subsp. sepedonicus using rep-PCR technique.

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    Fousek, J; Mráz, I

    2003-01-01

    Testing of 23 isolates of Clavibacter michiganensis subsp. sepedonicus for analysis by rep-PCR (using BOX, ERIC, REP primer sets) was used for the purpose of localization of genetic markers for fluid and/or nonfluid strains. None of the primer sets was successful in detecting genetic differences between the isolates and no polymorphism was generated.

  18. Phenotypic characterization and ERIC-PCR based genotyping of Paenibacillus larvae isolates recovered from American foulbrood outbreaks in honey bees from Italy.

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    Bassi, Stefano; Formato, Giovanni; Milito, Marcella; Trevisiol, Karin; Salogni, Cristian; Carra, Elena

    2015-03-01

    Paenibacillus larvae is the etiological agent of American foulbrood (AFB), a widespread and severe bacterial brood disease of honey bees. The genomic characterization of P. larvae strains by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) is able to differentiate four genotypes (ERIC I, ERIC II, ERIC III, ERIC IV). The information on the presence of P. larvae ERIC genotypes worldwide is few. We have characterized P. larvae strains isolated in Italy from AFB outbreaks to obtain information on ERIC genotypes and phenotypes of the strains circulating in the country. A total of 117 P. larvae isolates from 115 AFB outbreaks occurring in 2008-2012 were subjected to phenotypic and genetic characterization. The genomic characterization allowed the identification of ERIC I and ERIC II genotypes. Examining the data of Northern and Central Italy separately it was noted that in Northern Italy most outbreaks were caused by the ERIC I genotype (78.6%), followed by the ERIC II genotype (18.6%) and by co-infections (ERIC I + ERIC II) (2.6%). In Central Italy, only outbreaks caused by the ERIC I genotype were observed. With regard to phenotypic characteristics all examined strains of ERIC II genotype fermented fructose while no strains of ERIC I genotype possessed this ability. Both P. larvae ERIC I and ERIC II genotypes were isolated from the AFB outbreaks, but ERIC II genotype was isolated only in Northern Italy. The fermentation of fructose seems to be a genotype-specific biochemical marker.

  19. The Eric Cantor Upset

    DEFF Research Database (Denmark)

    Ashbee, Edward

    2014-01-01

    US update. The surprise primary defat of Eric Cantor, which shows that the Tea Party is still in business......US update. The surprise primary defat of Eric Cantor, which shows that the Tea Party is still in business...

  20. Eric Foner

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    Andrew Hammond

    2014-10-01

    Full Text Available Eric Foner is DeWitt Clinton Professor of History at Columbia University. Born in New York City to a family that included union organisers, political activists, and historians – his father Jack was a scholar of military history – Foner has gone on to become one of the leading historians of his generation. His most recent book, for example, The Fiery Trial: Abraham Lincoln and American Slavery (2010, won the Pulitzer Prize, the Bancroft Prize, and the Lincoln Prize, while he has been one of only two figures to have been elected  President of  the American Historical Association, Society of American Historians, and the Organisation of American Historians. Previous works have included Free Soil, Free Labour, Free Men: The Ideology of the Republican Party Before the Civil War (1970, Reconstruction: America’s Unfinished Revolution, 1863-1877 (1988, and The Story of American Freedom (1998. While two strands run throughout his intellectual trajectory, the first being the abolition and legacy of slavery, it is the second theme that I wish to take up here.Photo credit:  Daniella Zalcman

  1. República Argentina

    OpenAIRE

    Centro de Estudios Sudamericanos

    2008-01-01

    El sistema de gobierno en la Argentina adopta la forma representativa, republicana y federal (Constitución Nacional, art. 1º).Por su extensión -que corresponden al Continente Americano; al Continente Antártico (incluyendo las Islas Orcadas del Sur) y las islas australes (Georgias del Sur y Sandwich del Sur)- ocupa el cuarto lugar entre los países americanos (después de Canadá, Estados Unidos de América y la República Federativa del Brasil) y el séptimo a nivel mundial. Con una población de má...

  2. República Argentina

    OpenAIRE

    Centro de Estudios Sudamericanos (CENSUD)

    2008-01-01

    El sistema de gobierno en la Argentina adopta la forma representativa, republicana y federal (Constitución Nacional, art. 1º).Por su extensión -que corresponden al Continente Americano; al Continente Antártico (incluyendo las Islas Orcadas del Sur) y las islas australes (Georgias del Sur y Sandwich del Sur)- ocupa el cuarto lugar entre los países americanos (después de Canadá, Estados Unidos de América y la República Federativa del Brasil) y el séptimo a nivel mundial. Con una población de má...

  3. Comparison of a Commercially Available Repetitive-Element PCR System (DiversiLab) with PCR Ribotyping for Typing of Clostridium difficile Strains ▿

    OpenAIRE

    Eckert, C; Van Broeck, J; Spigaglia, P.; Burghoffer, B.; Delmée, M; Mastrantonio, P; Barbut, F

    2011-01-01

    This study compared a repetitive-element PCR (rep-PCR) method (DiversiLab system) to PCR ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR ribotype 027 isolates tested, different rep types could be distinguished. rep-PCR showed a higher discriminatory power than PCR ribotyping. Nevertheless, this method requires technical skill, and visual interpretation of rep-PCR fingerprint patterns may be difficult.

  4. Comparison of a Commercially Available Repetitive-Element PCR System (DiversiLab) with PCR Ribotyping for Typing of Clostridium difficile Strains ▿

    OpenAIRE

    Eckert, C.; Van Broeck, J.; Spigaglia, P.; Burghoffer, B; Delmée, M; Mastrantonio, P; Barbut, F

    2011-01-01

    This study compared a repetitive-element PCR (rep-PCR) method (DiversiLab system) to PCR ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR ribotype 027 isolates tested, different rep types could be distinguished. rep-PCR showed a higher discriminatory power than PCR ribotyping. Nevertheless, this method requires technical skill, and visual interpretation of rep-PCR fingerprint patterns may be difficult.

  5. RepA and RepB exert plasmid incompatibility repressing the transcription of the repABC operon.

    Science.gov (United States)

    Pérez-Oseguera, Angeles; Cevallos, Miguel A

    2013-11-01

    Rhizobium etli CFN42 has a multipartite genome composed of one chromosome and six large plasmids with low copy numbers, all belonging to the repABC plasmid family. All elements essential for replication and segregation of these plasmids are encoded within the repABC operon. RepA and RepB direct plasmid segregation and are involved in the transcriptional regulation of the operon, and RepC is the initiator protein of the plasmid. Here we show that in addition to RepA (repressor) and RepB (corepressor), full transcriptional repression of the operon located in the symbiotic plasmid (pRetCFN42d) of this strain requires parS, the centromere-like sequence, and the operator sequence. However, the co-expression of RepA and RepB is sufficient to induce the displacement of the parental plasmid. RepA is a Walker-type ATPase that self associates in vivo and in vitro and binds specifically to the operator region in its RepA-ADP form. In contrast, RepA-ATP is capable of binding to non-specific DNA. RepA and RepB form high molecular weight DNA-protein complexes in the presence of ATP and ADP. RepA carrying ATP-pocket motif mutations induce full repression of the repABC operon without the participation of RepB and parS. These mutants specifically bind the operator sequence in their ATP or ADP bound forms. In addition, their expression in trans exerts plasmid incompatibility against the parental plasmid. RepA and RepB expressed in trans induce plasmid incompatibility because of their ability to repress the repABC operon and not only by their capacity to distort the plasmid segregation process.

  6. Grabbing ERIC by the Tail: Introducing the ERIC Commissioned Papers.

    Science.gov (United States)

    Lankes, R. David

    2001-01-01

    Explains the origin of the ERIC (Educational Resources Information Center) commissioned papers that appear in this journal issue that assess the ERIC program. Discusses the need to unify the system by transforming the database into a knowledge base with a unified metadata framework that would include a variety of multimedia materials. (LRW)

  7. Bibliographical References from ERIC Citations.

    Science.gov (United States)

    Banta, Trudy W.; Bowlby, Sylva M.

    The educational document and journal citations found in the ERIC (Educational Resources Information Center) system are explained and the proper translation into standard bibliographic form is given for the usual and irregular cases. Section 1 explains the meaning of each part of an ERIC citation and, in parallel fashion, the parts and order of a…

  8. Indole acetic acid production by fluorescent Pseudomonas spp. from the rhizosphere of Plectranthus amboinicus (Lour.) Spreng. and their variation in extragenic repetitive DNA sequences.

    Science.gov (United States)

    Sethia, Bedhya; Mustafa, Mariam; Manohar, Sneha; Patil, Savita V; Jayamohan, Nellickal Subramanian; Kumudini, Belur Satyan

    2015-06-01

    Fluorescent Pseudomonas (FP) is a heterogenous group of growth promoting rhizobacteria that regulate plant growth by releasing secondary metabolic compounds viz., indole acetic acid (IAA), siderophores, ammonia and hydrogen cyanide. In the present study, IAA producing FPs from the rhizosphere of Plectranthus amboinicus were characterized morphologically, biochemically and at the molecular level. Molecular identification of the isolates were carried out using Pseudomonas specific primers. The effect of varying time (24, 48, 72 and 96 h), Trp concentrations (100, 200, 300, 400 and 500 μg x ml(-1)), temperature (10, 26, 37 and 50 ± 2 degrees C) and pH (6, 7 and 8) on IAA production by 10 best isolates were studied. Results showed higher IAA production at 72 h incubation, at 300 μg x ml(-1) Trp concentration, temperature 26 ± 2 degrees C and pH 7. TLC with acidified ethyl acetate extract showed that the IAA produced has a similar Rf value to that of the standard IAA. Results of TLC were confirmed by HPLC analysis. Genetic diversity of the isolates was also studied using 40 RAPD and 4 Rep primers. Genetic diversity parameters such as dominance, Shannon index and Simpson index were calculated. Out of 40 RAPD primers tested, 9 (2 OP-D series and 7 OP-E series) were shortlisted for further analysis. Studies using RAPD, ERIC, BOX, REP and GTG5 primers revealed that isolates exhibit significant diversity in repetitive DNA sequences irrespective of the rhizosphere.

  9. Stability of repetitive-sequence PCR patterns with respect to culture age and subculture frequency.

    Science.gov (United States)

    Kang, Hyunseok Peter; Dunne, W Michael

    2003-06-01

    To examine the stability of repetitive-sequence (rep) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted from the cultures after 24, 48, and 72 h of incubation at 35 degrees C. In addition, the same species were subcultured to fresh blood plates daily and DNA was extracted from the cultures after growth of 5, 10, and 15 subcultures, respectively. rep PCR analysis demonstrated that all rep PCR fingerprints from a single species were identical.

  10. Facilitated Molecular Typing of Shigella Isolates Using ERIC-PCR

    Science.gov (United States)

    Kosek, Margaret; Yori, Pablo Peñataro; Gilman, Robert H.; Vela, Henry; Olortegui, Maribel Paredes; Chavez, Cesar Banda; Calderon, Maritza; Bao, Juan Perez; Hall, Eric; Maves, Ryan; Burga, Rosa; Sanchez, Graciela Meza

    2012-01-01

    To evaluate the performance of enterobacterial repetitive intergenic sequence-based polymerase chain reaction (ERIC-PCR) typing versus the current standard for the typing of Shigella pulsed gel electrophoresis (PFGE), we typed 116 Shigella isolates from a village in an endemic setting over a 20-month period using both methods. PFGE identified 37 pulse types and had a discrimination index of 0.925 (95% confidence interval = 0.830–1.00), whereas ERIC-PCR identified 42 types and had a discrimination index of 0.961 (95% confidence interval = 0.886–1.00). PFGE and ERIC-PCR showed a 90.4% correlation in the designation of isolates as clonal or non-clonal in pairwise comparisons. Both systems were highly reproducible and provided highly similar and supplementary data compared with serotyping regarding the transmission dynamics of shigellosis in this community. ERIC-PCR is considerably more rapid and inexpensive than PFGE and may have a complementary role to PFGE for initial investigations of hypothesized outbreaks in resource-limited settings. PMID:22665611

  11. Remembering Eric Hobsbawm

    Directory of Open Access Journals (Sweden)

    Wade Matthews

    2012-10-01

    Full Text Available Eric Hobsbawm died in London on October 1, 2012. He was among the leading historians of the twentieth-century. Indeed at the time of his death he was almost universally described as “arguably Britain’s most respected historian,” (Kettle and Wedderburn, 2012 and this despite his long-standing commitment to Marxism. It’s not hard to see why. His productivity was extraordinary, stretching from the 1940s to the 2010s, and his intellectual range was immense, moving effortlessly from the Swing Riots and the Industrial Revolution to popular rebellion and global terrorism. In these terms, comparisons don’t come easily to mind.

  12. Utshitsja drug u druga / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2005-01-01

    Haridus- ja teadusminister Mailis Reps uuendustest koolide õppekavades, õpetajate täienduskoolituse tähtsusest, kutsehariduse arengust, tasuta toitlustamisest põhi- ja kutsekoolides, õpetajate palkadest

  13. Utshitsja drug u druga / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2005-01-01

    Haridus- ja teadusminister Mailis Reps uuendustest koolide õppekavades, õpetajate täienduskoolituse tähtsusest, kutsehariduse arengust, tasuta toitlustamisest põhi- ja kutsekoolides, õpetajate palkadest

  14. Information for Education Writers from ERIC.

    Science.gov (United States)

    Harrison, Charles H.

    The Educational Resources Information Center (ERIC) system provides information to education writers for newspapers, magazines, radio, and television. ERIC can be used to find the names of reliable sources in a given field, as well as background information for an in-depth article or broadcast. The ERIC collection of documents on microfiche is…

  15. RepARK--de novo creation of repeat libraries from whole-genome NGS reads.

    Science.gov (United States)

    Koch, Philipp; Platzer, Matthias; Downie, Bryan R

    2014-05-01

    Generation of repeat libraries is a critical step for analysis of complex genomes. In the era of next-generation sequencing (NGS), such libraries are usually produced using a whole-genome shotgun (WGS) derived reference sequence whose completeness greatly influences the quality of derived repeat libraries. We describe here a de novo repeat assembly method--RepARK (Repetitive motif detection by Assembly of Repetitive K-mers)--which avoids potential biases by using abundant k-mers of NGS WGS reads without requiring a reference genome. For validation, repeat consensuses derived from simulated and real Drosophila melanogaster NGS WGS reads were compared to repeat libraries generated by four established methods. RepARK is orders of magnitude faster than the other methods and generates libraries that are: (i) composed almost entirely of repetitive motifs, (ii) more comprehensive and (iii) almost completely annotated by TEclass. Additionally, we show that the RepARK method is applicable to complex genomes like human and can even serve as a diagnostic tool to identify repetitive sequences contaminating NGS datasets.

  16. A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST

    Directory of Open Access Journals (Sweden)

    Roberto Viau

    2017-02-01

    Full Text Available Molecular typing using repetitive sequenced-based PCR (rep-PCR and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST. In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.

  17. República del Paraguay

    OpenAIRE

    Centro de Estudios Sudamericanos

    2008-01-01

    La República del Paraguay adopta para su gobierno la democracia representativa, participativa y pluralista, fundada en el reconocimiento de la dignidad humana. Está ubicado en el corazón de América del Sur, dividido por el Río Paraguay en dos regiones, Oriental y Occidental o Chaco. Es un país mediterráneo, ya que no posee costas marítimas, así como lo es su vecino Bolivia, pero riegan sus costas dos ríos importantes, el Paraguay y el Paraná que desembocan en el Río de la Plata y que son util...

  18. ERIC: Mission, Structure, and Resources.

    Science.gov (United States)

    Robbins, Jane B.

    2001-01-01

    Provides an overview of the mission, structure, and resource base of the Educational Resources Information Center (ERIC). Highlights include problems in meeting the information needs of a wide variety of educational practitioners as part of the mission; structure, based on organizational decentralization; and resources that are limited by…

  19. Coaching for Tests. ERIC Digest.

    Science.gov (United States)

    Wildemuth, Barbara

    The term "coaching" applies to a variety of types of test preparation programs which vary in length, instructional method, and content. Most research on the effectiveness of coaching has examined the Scholastic Aptitude Test (SAT), a measure of academic abilities used to predict college performance. This ERIC Digest reviews studies of…

  20. On Standardized Testing. ERIC Digest.

    Science.gov (United States)

    Perrone, Vito

    This ERIC Digest was adapted from the Association for Childhood Education International's (ACEI) 1991 position paper on standardized testing. Since the publication of "A Nation at Risk" in 1983, standardized testing programs have expanded greatly. Tests may be of pencil-and-paper or performance-oriented varieties. The purposes of tests…

  1. Bacterial repetitive extragenic palindromic sequences are DNA targets for Insertion Sequence elements

    Directory of Open Access Journals (Sweden)

    Pareja Eduardo

    2006-03-01

    Full Text Available Abstract Background Mobile elements are involved in genomic rearrangements and virulence acquisition, and hence, are important elements in bacterial genome evolution. The insertion of some specific Insertion Sequences had been associated with repetitive extragenic palindromic (REP elements. Considering that there are a sufficient number of available genomes with described REPs, and exploiting the advantage of the traceability of transposition events in genomes, we decided to exhaustively analyze the relationship between REP sequences and mobile elements. Results This global multigenome study highlights the importance of repetitive extragenic palindromic elements as target sequences for transposases. The study is based on the analysis of the DNA regions surrounding the 981 instances of Insertion Sequence elements with respect to the positioning of REP sequences in the 19 available annotated microbial genomes corresponding to species of bacteria with reported REP sequences. This analysis has allowed the detection of the specific insertion into REP sequences for ISPsy8 in Pseudomonas syringae DC3000, ISPa11 in P. aeruginosa PA01, ISPpu9 and ISPpu10 in P. putida KT2440, and ISRm22 and ISRm19 in Sinorhizobium meliloti 1021 genome. Preference for insertion in extragenic spaces with REP sequences has also been detected for ISPsy7 in P. syringae DC3000, ISRm5 in S. meliloti and ISNm1106 in Neisseria meningitidis MC58 and Z2491 genomes. Probably, the association with REP elements that we have detected analyzing genomes is only the tip of the iceberg, and this association could be even more frequent in natural isolates. Conclusion Our findings characterize REP elements as hot spots for transposition and reinforce the relationship between REP sequences and genomic plasticity mediated by mobile elements. In addition, this study defines a subset of REP-recognizer transposases with high target selectivity that can be useful in the development of new tools for

  2. Minister Reps valetas kantslerit vallandades / Urmo Soonvald

    Index Scriptorium Estoniae

    Soonvald, Urmo, 1973-

    2006-01-01

    Kuigi haridus- ja teadusminister Mailis Reps ütles juulikuus, et ta loodab ministeeriumi kantsleri Jaan Kallasega kokkuleppele jõuda, oli tal selleks ajaks juba uus kantsler välja valitud, väidab autor

  3. República Federativa de Brasil

    OpenAIRE

    Centro de Estudios Sudamericanos

    2007-01-01

    Brasil, es un Estado organizado como una república federal, con una forma de gobierno democrática representativa y participativa. La república está organizada en 27 estados (26 más el Distrito Federal donde está ubicada la capital nacional, Brasilia). (Párrafo extraído del texto a modo de resumen) Instituto de Relaciones Internacionales (IRI)

  4. 空肠弯曲杆菌ERIC-PCR分型技术研究%Study on construction and application of ERIC-PCR method for typing Campylobacter jejuni

    Institute of Scientific and Technical Information of China (English)

    唐梦君; 高玉时; 张小燕; 陆俊贤; 施祖灏; 唐修君; 陈大伟

    2013-01-01

    目的:建立空肠弯曲杆菌肠杆菌科基因间重复一致序列PCR(ERIC-PCR)的分子分型技术,快速分析空肠弯曲杆菌指纹谱。方法本研究提取空肠弯曲杆菌ATCC33560的基因组DNA作为模板,采用对优化项目设置梯度其它条件不变的方法,对ERIC-PCR反应中的模板量、引物浓度和退火温度等进行优化,建立空肠弯曲杆菌的ERIC-PCR分型技术,并采用该技术对20株空肠弯曲杆菌的指纹图谱进行了分析。结果 ERIC-PCR反应体系中DNA模板量96ng/25μL,ERIC1、ERIC2引物浓度各0.8μmol/L,退火温度40℃时,指纹图谱条带清晰、明亮;20株空肠弯曲杆菌临床分离株ERIC-PCR指纹图谱差异较大,可在160bp~32000bp范围内出现2条~13条条带,分为14型。结论研究显示成功建立了空肠弯曲杆菌ERIC-PCR指纹图谱分型技术,应用该方法可从分子水平上对空肠弯曲杆菌基因组DNA进行快速指纹图谱分析,具有简便和快速等优点,能够为空肠弯曲杆菌流行病学调查提供科学依据。%Objective To generate an efifcient enterobacterial repetitive intergenic consensus sequence (ERIC-PCR) typing and analyze molecular type of different food-borne Campylobacter jejuni.Method Genomic DNA of Campylo-bacter jejuni reference strain ATCC33560 was used as the template for PCR. Target sequences in Campylobacter jejuni genomic DNA were amplified with the primers designed according to the reference. To obtain optimum fingerprint maps,template and primer concentration of the reaction system and annealing temperature of PCR,the factors to be optimized were designed in different concentration grads and other factors were ifxed. Then 20 Campylobacter jejuni isolates were classiifed by PCR.Result The optimal concentration of template DNA was 96ng/25mL,the concentration of primers was 0.8mmol/L each primer and annealing temperature of PCR was 40 ℃.The ERIC-PCR ifngerprint maps of different isolates

  5. Perspectives on ERIC and Adult Education

    Science.gov (United States)

    Imel, Susan

    2016-01-01

    Susan Imel is retired from Ohio State University. From December 1981 until December 2003, she was the Adult Education Specialist at the ERIC Clearinghouse on Adult, Career, and Vocational Education and Director from 1987 through 2003. This essay begins with a brief overview of the ERIC system including goals for the clearinghouses, followed by a…

  6. Likelihood methods and classical burster repetition

    CERN Document Server

    Graziani, C; Graziani, Carlo; Lamb, Donald Q

    1995-01-01

    We develop a likelihood methodology which can be used to search for evidence of burst repetition in the BATSE catalog, and to study the properties of the repetition signal. We use a simplified model of burst repetition in which a number N_{\\rm r} of sources which repeat a fixed number of times N_{\\rm rep} are superposed upon a number N_{\\rm nr} of non-repeating sources. The instrument exposure is explicitly taken into account. By computing the likelihood for the data, we construct a probability distribution in parameter space that may be used to infer the probability that a repetition signal is present, and to estimate the values of the repetition parameters. The likelihood function contains contributions from all the bursts, irrespective of the size of their positional errors --- the more uncertain a burst's position is, the less constraining is its contribution. Thus this approach makes maximal use of the data, and avoids the ambiguities of sample selection associated with data cuts on error circle size. We...

  7. Genomic fingerprinting Acinetobacter baumannii: amplification of multiple inter-repetitive extragenic palindromic sequences.

    Science.gov (United States)

    Sheehan, C; Lynch, M; Cullen, C; Cryan, B; Greer, P; Fanning, S

    1995-09-01

    Acinetobacter species are important nosocomial pathogens. A rapid and sensitive identification system, capable of providing strain identity at the genetic level, is required to identify outbreak strains and facilitate the early implementation of infection control procedures. Repetitive extragenic palindromic (REP) elements, have been identified in numerous bacteria and these genomic sequences provide useful targets for DNA amplification. A method for amplifying inter-REP DNA sequences, REP-multiple arbitrary amplicon profiling (REP-MAAP), is described and applied to 29 Acinetobacter baumannii from clinical samples. Amplified polymorphic DNA patterns were demonstrated for all isolates and those displaying identical REP-MAAP patterns were considered identical at the genetic level. In the spring of 1993, 10 intensive care unit patients had endotracheal colonization with A. baumannii (five with REP-MAAP I and five with REP-MAAP II patterns). These findings suggested nosocomial transmission of organisms which was terminated by standard infection control measures. No further A. baumannii were detected until the winter of 1993 when isolates of different REP-MAAP groups emerged, suggesting that factors other than nosocomial transmission were implicated.

  8. República del Perú

    OpenAIRE

    Centro de Estudios Sudamericanos

    2006-01-01

    La República del Perú es un Estado organizado como una república unitaria, con una forma de gobierno democrática representativa y participativa. La población es de 25.662 millones de habitantes, siendo el 47% de origen indígena. La población urbana representa el 72,3% y la rural el 27,7% de la población total. Las mayores concentraciones de población se encuentran en la franja costera y en algunos núcleos urbanos de la sierra andina. La densidad poblacional es de 19 habitantes por k...

  9. The replication origin of a repABC plasmid

    Directory of Open Access Journals (Sweden)

    Cevallos Miguel A

    2011-06-01

    Full Text Available Abstract Background repABC operons are present on large, low copy-number plasmids and on some secondary chromosomes in at least 19 α-proteobacterial genera, and are responsible for the replication and segregation properties of these replicons. These operons consist, with some variations, of three genes: repA, repB, and repC. RepA and RepB are involved in plasmid partitioning and in the negative regulation of their own transcription, and RepC is the limiting factor for replication. An antisense RNA encoded between the repB-repC genes modulates repC expression. Results To identify the minimal region of the Rhizobium etli p42d plasmid that is capable of autonomous replication, we amplified different regions of the repABC operon using PCR and cloned the regions into a suicide vector. The resulting vectors were then introduced into R. etli strains that did or did not contain p42d. The minimal replicon consisted of a repC open reading frame under the control of a constitutive promoter with a Shine-Dalgarno sequence that we designed. A sequence analysis of repC revealed the presence of a large A+T-rich region but no iterons or DnaA boxes. Silent mutations that modified the A+T content of this region eliminated the replication capability of the plasmid. The minimal replicon could not be introduced into R. etli strain containing p42d, but similar constructs that carried repC from Sinorhizobium meliloti pSymA or the linear chromosome of Agrobacterium tumefaciens replicated in the presence or absence of p42d, indicating that RepC is an incompatibility factor. A hybrid gene construct expressing a RepC protein with the first 362 amino acid residues from p42d RepC and the last 39 amino acid residues of RepC from SymA was able to replicate in the presence of p42d. Conclusions RepC is the only element encoded in the repABC operon of the R. etli p42d plasmid that is necessary and sufficient for plasmid replication and is probably the initiator protein. The ori

  10. RepA-WH1 prionoid

    Science.gov (United States)

    de la Espina, Susana Moreno-Díaz; Fernández-Tresguerres, M Elena; Gasset-Rosa, Fátima

    2011-01-01

    The intricate complexity at the molecular and cellular levels of the processes leading to the development of amyloid proteinopathies is somehow counterbalanced by their common, universal structural basis. The later has fueled the quest for suitable model systems to study protein amyloidosis under quasi-physiological conditions in vitro and in simpler organisms in vivo. Yeast prions have provided several of such model systems, yielding invaluable insights on amyloid structure, dynamics and transmission. However, yeast prions, unlike mammalian PrP, do not elicit any proteinopathy. We have recently reported that engineering RepA-WH1, a bacterial DNA-toggled protein conformational switch (dWH1→mWH1) sharing some analogies with nucleic acid-promoted PrPC→PrPSc replication, enables control on protein amyloidogenesis in vitro. Furthermore, RepA-WH1 gives way to a non-infectious, vertically-transmissible (from mother to daughter cells) amyloid proteinopathy in Escherichia coli. RepA-WH1 amyloid aggregates efficiently promote aging in bacteria, which exhibit a drastic lengthening in generation time, a limited number of division cycles and reduced fitness. The RepA-WH1 prionoid opens a direct means to untangle the general pathway(s) for protein amyloidosis in a host with reduced genome and proteome. PMID:21293179

  11. Genetic Diversity and Evidence for Transmission of Streptococcus mutans by DiversiLab rep-PCR.

    Science.gov (United States)

    Momeni, Stephanie S; Whiddon, Jennifer; Cheon, Kyounga; Ghazal, Tariq; Moser, Stephen A; Childers, Noel K

    2016-09-01

    This two-part study investigated the genetic diversity and transmission of Streptococcus mutans using the DiversiLab repetitive extragenic palindromic PCR (rep-PCR) approach. For children with S. mutans and participating household members, analysis for evidence of unrelated child-to-child as well as intra-familial transmission was evaluated based on commonality of genotypes. A total of 169 index children and 425 household family members from Uniontown, Alabama were evaluated for genetic diversity using rep-PCR. Thirty-four unique rep-PCR genotypes were observed for 13,906 S. mutans isolates. For transmission, 117 child and household isolates were evaluated for shared genotype (by child and by genotype cases, multiple matches possible for each child). Overall, children had 1-9 genotypes and those with multiple genotypes were 2.3 times more likely to have caries experience (decayed, missing and filled teeth/surfaces>0). Only 28% of children shared all genotypes within the household, while 72% had at least 1 genotype not shared with anyone in the household. Children had genotype(s) not shared with any household members in 157 cases. In 158 cases children and household members shared a genotype in which 55% (87/158 cases) were shared with more than one family member. Children most frequently shared genotypes with their mothers (54%; 85/158), siblings (46%; 72/158) and cousins (23%; 37/158). A reference library for S. mutans for epidemiological surveillance using the DiversiLab rep-PCR approach is detailed. The genetic diversity of S. mutans in this population demonstrated frequent commonality of genotypes. Evidence for both child-to-child and intra-familial transmission of S. mutans was observed by rep-PCR.

  12. Diversity of Enterococcus faecalis Genotypes from Multiple Oral Sites Associated with Endodontic Failure Using Repetitive Sequence-based Polymerase Chain Reaction and Arbitrarily Primed Polymerase Chain Reaction.

    Science.gov (United States)

    Delboni, Maraísa G; Gomes, Brenda P F A; Francisco, Priscila A; Teixeira, Fabrício B; Drake, David

    2017-03-01

    The aim of this study was to evaluate the diversity and similarity of Enterococcus faecalis genotype isolates from multiple oral sites using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction (AP-PCR). Forty-two endodontically treated teeth with apical periodontitis were selected. A total of 126 microbial samples were collected from 3 different sites (saliva, pulp chamber, and root canals, all n = 42) during the nonsurgical retreatment procedures. After growth on m-Enterococcus agar, the colonies were isolated, characterized as gram-positive catalase negative cocci, and identified using an API 20 Strep kit (bioMérieux, Marcy-l'Etoile, France). Seventy-four colonies from 10 patients were confirmed as E. faecalis by polymerase chain reaction (16S ribosomal RNA). Repetitive sequence-based polymerase chain reactions using ERIC and AP-PCR using RW3A primers were performed in all 74 colonies. Fingerprints were analyzed and separated into genotypic groups based on the Dice coefficient percentage of similarity (82% or greater) as determined by ERIC reproducibility assays involving E. faecalis controls. Seven different E. faecalis genotypes (GTs) (GT1 = 27%, GT2 = 17.6%, GT3 = 1.3%, GT4 = 18.9%, GT5 = 9.5%, GT6 = 14.9%, and GT7 = 10.8%) were observed in different subjects and oral sites associated with endodontic failure. Remarkably, in 4 of 5 patients, the same GTs present in the infected root canals were also isolated from either the pulp chamber or the saliva samples. In particular, GT6 was detected in all 3 oral sites of patient 37. E. faecalis GTs isolated from saliva, the pulp chamber, and the root canal were similar using the Rep-PCR and AP-PCR methods. These findings suggest that coronal microleakage is a conceivable cause of endodontic failure. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  13. Research on Spelling in Primary Grades (ERIC).

    Science.gov (United States)

    Sensenbaugh, Roger

    1993-01-01

    Presents annotations of nine conference papers and practica in the ERIC database that deal with spelling research in the primary grades. Notes that the entries in the annotated bibliography were published between 1990 and 1992. (RS)

  14. Evaluation of the ERIC-PCR as a probable method to differentiate Avibacterium paragallinarum serovars.

    Science.gov (United States)

    Hellmuth, Julius Eduard; Hitzeroth, Arina Corli; Bragg, Robert Richard; Boucher, Charlotte Enastacia

    2017-06-01

    Infectious coryza, an upper respiratory tract disease in chickens, caused by Avibacterium paragallinarum, leads to huge economic losses. The disease is controlled through vaccination; but vaccination efficacy is dependent on correct identification of the infecting serovar, as limited cross-protection is reported amongst some serovars. Current identification methods include the heamagglutination inhibition test, which is demanding and could be subjective. To overcome this, molecular typing methods proposed are the Multiplex polymerase chain reaction (PCR) and Restriction Fragment Length Polymorphism-PCR, but low reproducibility is reported. Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR has been suggested for molecular groupings of various bacterial species. This study focuses on evaluating the ERIC-PCR as a probable method to differentiate between different Av. paragallinarum serovars by grouping with reference isolates, based on clonal relations. The ERIC-PCR was performed on 12 reference isolates and 41 field isolates originating from South Africa and South America. The data indicate that the ERIC-PCR is not ideal for the differentiation or for molecular typing of Av. paragallinarum serovars, as no correlation is drawn upon comparison of banding patterns of field isolates and reference strains. However, the results do indicate isolates from the same origin sharing unique banding patterns, indicating potential clonal relationship; but when compared to the reference isolates dominant in the specific area, no correlation could be drawn. Furthermore, although the ERIC-PCR serves a purpose in epidemiological studies, it has proved to have little application in differentiating amongst serovars of Av. paragallinarum and to group untyped field strains with known reference strains.

  15. RAPD- and ERIC-Based Typing of Clinical and Environmental Pseudomonas aeruginosa Isolates.

    Science.gov (United States)

    Auda, Ibtesam Ghadban; Al-Kadmy, Israa M S; Kareem, Sawsan Mohammed; Lafta, Aliaa Khyuon; A'Affus, Mustafa Hussein Obeid; Khit, Ibrahim Abd Aloahd; Al Kheraif, Abdulaziz Abdullah; Divakar, Darshan Devang; Ramakrishnaiah, Ravikumar

    2017-03-01

    Pseudomonas aeruginosa is a major cause of nosocomial infection in children and adults, resulting in significant morbidity and mortality due to its ability to acquire drug resistance. The ability of P. aeruginosa in the environment to cause infection in individuals has been reported previously; henceforth, surveillance of the emergence and transmission of P. aeruginosa strains among patients is important for infection control in a clinical setup. Various gene-typing methods have been used for epidemiological typing of P. aeruginosa isolates for the purpose of surveillance. In this work, the suitability and comparability of two typing methods, enterobacterial repetitive intergenic consensus (ERIC)-PCR and random amplification of polymorphic DNA (RAPD)-PCR fingerprinting, were studied to characterize P. aeruginosa strains isolated from clinical and environmental sources. Forty-four clinical and environmental bacterial isolates of P. aeruginosa were collected between October 2015 and January 2016. DNA extraction, ERIC-PCR and RAPD-PCR, agarose gel electrophoresis, and phylogenetic analyses were carried using the unweighted pair-group method with mean. RAPD typing revealed less clonality among clinical isolates, whereas the ERIC method showed greater similarity in comparison with RAPD. Environmental isolates, however, showed greater similarity using RAPD compared with ERIC typing. With only a few exceptions, most clinical isolates were distinct from environmental isolates, irrespective of the typing method. In conclusion, both the RAPD and ERIC typing methods proved to be good tools in understanding clonal diversity. The results also suggest that there is no relationship between clinical and environmental isolates. The absence of clonality among the clinical isolates may indicate that most P. aeruginosa infection cases could be endemic and not epidemic and that endemic infections may be due to nonclonal strains of P. aeruginosa.

  16. Use of Long-Range Repetitive Element Polymorphism-PCR To Differentiate Bacillus anthracis Strains

    OpenAIRE

    Brumlik, Michael J.; Szymajda, Urszula; Zakowska, Dorota; Liang, Xudong; Redkar, Rajendra J.; Patra, Guy; Del Vecchio, Vito G.

    2001-01-01

    The genome of Bacillus anthracis is extremely monomorphic, and thus individual strains have often proven to be recalcitrant to differentiation at the molecular level. Long-range repetitive element polymorphism-PCR (LR REP-PCR) was used to differentiate various B. anthracis strains. A single PCR primer derived from a repetitive DNA element was able to amplify variable segments of a bacterial genome as large as 10 kb. We were able to characterize five genetically distinct groups by examining 10...

  17. O MAL EM ERIC WEIL

    Directory of Open Access Journals (Sweden)

    Daniel Benevides Soares

    2014-12-01

    Full Text Available O mal ocupa a condição de problema para a filosofia desde a Antiguidade. Passando pelas ponderações dos estóicos e de Santo Agostinho, tratado no contexto da teodiceia por Leibniz, o mal aporta como objeto de preocupação para a moral em Kant. Muito conhecido por sua definição como um kantiano pós-hegeliano, é com Kant, mas indo além dele que Weil trata do mal, pensando-o como formas de violência. Considerada como o outro do sentido e da razão, a violência se manifesta de maneiras variadas. Nosso intuito é determinar o conceito de mal em Eric Weil enquanto forma de violência. Para isso, nos propomos a situar no esquema conceitual da violência duas formas de mal discutidas por Weil nos seus textos: o mal radical e o mal diabólico.

  18. ERIC User Services: Changes and Evaluation for the Future.

    Science.gov (United States)

    Hsieh-Yee, Ingrid

    2001-01-01

    Presents findings from a study of ERIC (Educational Resources Information Center) user services and evaluation efforts of them. Identifies changes that would increase ERIC's competitive edge in the networked environment and recommends an evaluation framework for ERIC to monitor progress to its strategic goals. (LRW)

  19. 76 FR 39844 - Action Affecting Export Privileges; ERIC COHEN

    Science.gov (United States)

    2011-07-07

    .... 783 (2002)), as extended most recently by the Notice of August 17, 2010 (75 FR 50,681 (Aug. 16, 2010... Bureau of Industry and Security Action Affecting Export Privileges; ERIC COHEN 10-BIS-0005 Eric Cohen, 1631 East 10th Street, Brooklyn, NY 11223, Respondent. Order Relating to Eric Cohen The Bureau...

  20. Reminiscences from the Field: The Continuing Story of ERIC.

    Science.gov (United States)

    Colker, Laura J.

    The Educational Resources Information Center (ERIC) was born in the mid-1960s and is today the world's largest and most popular educational database. The history of ERIC's early development has been told in Del Trester's 1981 narrative, "ERIC--The First Fifteen Years: 1964-1979, A History of the Educational Resources Information Center."…

  1. Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker.

    Science.gov (United States)

    Silva, G F; Paixão, R D V; Queiroz, C B; Santana, M F; Souza, A; Sousa, N R; Hanada, R E; Gasparotto, L

    2014-09-26

    The Enterobacterial repetitive intergenic consensus (ERIC) marker was used to analyze the genetic variability of Mycosphaerella fijiensis, the causative agent of Black Sigatoka disease in banana plants. A total of 123 isolates were used, which were divided into populations based on their original hosts and collection sites in Brazil. A total of 9 loci were amplified, 77.8% of which were found to be polymorphic. The genetic diversity found in the population was 0.20. Analysis of molecular variance (AMOVA) demonstrated that the highest level of genetic variation is within populations. Cluster analysis revealed three main groups in Brazil, with no correlation between geographic and genetic distance.

  2. [Characterization of rhizobia causing nodules on leguminous trees native to Uruguay using the rep-PCR technique].

    Science.gov (United States)

    Rodríguez, A; Frioni, L

    2003-01-01

    Methods for identifying and following microorganisms in the environment such as soils, water and plant association have been highly developed in recent years. In this study, we used rep-PCR for the characterization of Rhizobium bacteria isolated from legume trees native of Uruguay which permitted to follow them in plant inoculation assays. Among the primers used, ERIC and BOX1AR, the latter allowed the differentiation of isolates from different legumes and the finding of a high level of homology among rhizobia that nodulate the same legume species. Besides, it demonstrated that the profiles of the isolates recovered from nodules of Acacia caven, were identical to the ones used as inoculants; this confirmed the efficiency of these methods to follow these diazotrophs in the environment.

  3. de la República de Cuba

    Directory of Open Access Journals (Sweden)

    Daniel Ponce de León Lima

    2008-01-01

    Full Text Available Se presenta un esbozo de las estrategias a seguir para la inserción de la información edafológica en la Infraestructura de Datos Espaciales de la República de Cuba, a partir de un análisis del estado actual de desarrollo de la cartografía digital edafológica de Cuba y del desarrollo de bases de datos de la información de perfiles de suelo asociada. Se proponen una serie de tareas para lograr estos fines

  4. Mailis Reips : Prezhde vsego neobhodimo natshat / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2006-01-01

    Haridusminister Mailis Reps kakskeelse hariduse vajalikkusest vene õppekeelega koolides, noorte keeleoskusest, eesti keele õpetamise kvaliteedist, õpetajate eesti keele oskusest, õppetöö kvaliteedist

  5. Molecular characterization of Pasteurella multocida isolates obtained from poultry, ruminant, cats and dogs using RAPD and REP-PCR analysis

    Directory of Open Access Journals (Sweden)

    Hesamaddin Shirzad-Aski

    2016-09-01

    Full Text Available In the present study, Randomly Amplified Polymorphic DNA‌ (RAPD and Repetitive Extragenic Palindromic sequence-based Polymerase Chain Reaction (REP-PCR were used to characterize 131 isolates of Pasteurella multocida, originating from different healthy and diseased animal species obtained from several geographical regions of Iran. The RAPD and REP-PCR generated amplified products in the range of 300 to 3400 bp and 200 to 2850 bp, respectively. Among all of the P. multocida isolates, cluster analysis revealed that 63 clusters and nine untypable isolates and 81 clusters and six untypable isolates were produced with RAPD and REP-PCR methods, respectively. The results indicated that the REP-PCR method showed a slightly higher level of discrimination power in differentiating of P. multocida isolates as compared with RAPD. The results showed that a considerable level of genetic diversity exists among P. multocida isolates even in the isolates with the same animal or geographical origins. There was no host- and region-specific pattern. In addition, the isolates obtained from the healthy and diseased animal did not reveal any correlation genotypic profiles, which could be supported by the hypothesis that P. multocida is a strictly opportunistic pathogen. In conclusion, because of a large amount of genetic heterogeneityin the P. multocida isolates, Pasteurellosis may be caused by different clones in the same herd or animal.

  6. Repetitive maladaptive behavior: beyond repetition compulsion.

    Science.gov (United States)

    Bowins, Brad

    2010-09-01

    Maladaptive behavior that repeats, typically known as repetition compulsion, is one of the primary reasons that people seek psychotherapy. However, even with psychotherapeutic advances it continues to be extremely difficult to treat. Despite wishes and efforts to the contrary repetition compulsion does not actually achieve mastery, as evidenced by the problem rarely resolving without therapeutic intervention, and the difficulty involved in producing treatment gains. A new framework is proposed, whereby such behavior is divided into behavior of non-traumatic origin and traumatic origin with some overlap occurring. Repetitive maladaptive behavior of non-traumatic origin arises from an evolutionary-based process whereby patterns of behavior frequently displayed by caregivers and compatible with a child's temperament are acquired and repeated. It has a familiarity and ego-syntonic aspect that strongly motivates the person to retain the behavior. Repetitive maladaptive behavior of traumatic origin is characterized by defensive dissociation of the cognitive and emotional components of trauma, making it very difficult for the person to integrate the experience. The strong resistance of repetitive maladaptive behavior to change is based on the influence of both types on personality, and also factors specific to each. Psychotherapy, although very challenging at the best of times, can achieve the mastery wished and strived for, with the aid of several suggestions provided.

  7. [The experience of implementation of REP-u RAPD-polymerase chain reaction in epidemiologic characteristic of nosocomial isolates Pseudomonas aeruginosa].

    Science.gov (United States)

    Kuznetsova, M V; Maksimova, A V; Karpunina, T I

    2015-03-01

    The article presents comparative evaluation of diagnostic value of technique REP- u RAPD-polymerase chain reaction applied under genetic typing of clinical isolates of Pseudomonas Aeruginosa. The strains are isolated in different hospital departments of medical institutions in adult (8 medical institutions; n = 145) and children (5 medical institutions; n = 151) medical networks. The results of study demonstrated different boundary capacity of three reactions. The Simpson discrimination index made up to 0.993, 0.875 and 0.639 for RAPD-, ERIC- and BOX-polymerase chain reaction correspondingly. The RAPD-polymerase chain reaction makes it possible to detect individual characteristics of strains. Out of two alternatives the REP-polymerase chain reaction demonstrated its advantage, besides only with one primer ERIC2. The BOX-polymerase chain reaction has a least discriminating capacity under typing of isolates P. aeruginosa, detecting only species' characteristics. The clinical strains P. aeruginosa are distributed on 24 genome groups and 52 isolates had individual genotypes. The evaluation of results of genetic typing permitted to point out both similarity of tendencies in propagation of strains of P. aeruginosa among hospitalized adults and adolescents and specificity of detection in neonatal clinics. It is obvious that hospitals of different profiles, including departments of reanimation and intensive therapy represent specific ecological environment significantly different in its level of endogenous and exogenous infection.

  8. Comparative analysis of intestinal microbial community diversity between healthy and orally infected ducklings with Salmonella enteritidis by ERIC-PCR

    Institute of Scientific and Technical Information of China (English)

    Sheng-Yan Cao; De-Kang Zhu; Qi-Hui Luo; Xiao-Yue Chen; Ming-Shu Wang; An-Chun Cheng; Xue-Feng Qi; Xiao-Yan Yang; Shu-Xuan Deng; Nian-Chun Yin; Zhen-Hua Zhang; Deng-Chun Zhou

    2008-01-01

    AIM:To analyze the difference of intestinal microbial community diversity between healthy and (S.enteritidis)orally infected ducklings.METHODS:Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was applied to analyze the intestinal microbial community diversity and dynamic change including duodenum,jejunum,ileum,cecum and rectum from healthy ducklings and 7-dayoold ducklings after oral infection with S.enteritidis at different time points.RESULTS:The intestinal microbial community of the control healthy ducklings was steady and the ERIC-PCR band numbers of the control healthy ducklings were the least with rectum and were the most with caecum.ER[C-PCR bands of orally inoculated ducklings did not obviously change until 24 h after inoculation (p.i.).The numbers of the ERIC-PCR bands gradually decreased from 24 h to 72 h p.i.,and then,with the development of disease,the band numbers gradually increased until 6 d p.i.The prominent bacteria changed because of S.enteritidis infection and the DNAstar of staple of ERIC-PCR showed that aerobe and facultative aerobe (Escherichia coli,Shigella,Salmonella) became preponderant bacilli in the intestine of orally infected ducklings with SE.CONCLUSION:This study has provided significant data to clarify the intestinal microbial community diversity and dynamic change of healthy and S.enterltidis orally infected ducklings,and valuable insight into the pathogenesis of S.enteritidis infection in both human and animals.

  9. A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection

    Institute of Scientific and Technical Information of China (English)

    Jin-Long Yang; Ming-Shu Wang; An-Chun Cheng; Kang-Cheng Pan; Chuan-Feng Li; Shu-Xuan Deng

    2008-01-01

    AIM: To develop a simple and convenient method for extracting genomic DNA from intestinal microflora for enterobacterial repetitive intergenic consensus (ERIC)-PCR detection.METHODS: Five methods of extracting bacterial DNA,including Tris-EDTA buffer, chelex-100, ultrapure water,2% sodium dodecyl sulfate and 10% Triton-100 with and without sonication, were compared with the commercial fecal DNA extraction kit method, which is considered as the gold standard for DNA extraction. The comparison was based on the yield and purity of DNA and the indexes of the structure and property of micro-organisms that were reflected by ERIC-PCR.RESULTS: The yield and purity of DNA obtained by the chelex method was similar to that obtained with the fecal DNA kit. The ERIC-PCR results obtained for the DNA extracted by the chelex method and those obtained for DNA extracted with the fecal DNA kit were basically the same.CONCLUSION: The chelex method is recommended for ERIC-PCR experiments in view of its simplicity and costeffectiveness; and it is suitable for extracting total DNA from intestinal micro-organisms, particularly for handling a large number of samples.

  10. A dimeric Rep protein initiates replication of a linear archaeal virus genome: implications for the Rep mechanism and viral replication

    DEFF Research Database (Denmark)

    Oke, Muse; Kerou, Melina; Liu, Huanting

    2011-01-01

    that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between...... positioned active sites, each with a single tyrosine residue, work in tandem to catalyze DNA nicking and joining. We propose a novel mechanism for rudivirus DNA replication, incorporating the first known example of a Rep protein that is not linked to RCR. The implications for Rep protein function and viral...

  11. Effectiveness of enterobacterial repetitive intergenic consensus PCR and random amplified polymorphic DNA fingerprinting for Helicobacter pylori strain differentiation.

    Science.gov (United States)

    Finger, S Alison; Velapatiño, Billie; Kosek, Margaret; Santivañez, Livia; Dailidiene, Daiva; Quino, Willi; Balqui, Jacqueline; Herrera, Phabiola; Berg, Douglas E; Gilman, Robert H

    2006-07-01

    We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting cases of mixed Helicobacter pylori infection in Peruvian shantytown residents. H. pylori isolates from 63 participants were cultured, and five single colonies and a pool of additional colonies from each participant were analyzed by ERIC-PCR and by RAPD tests with four 10-nucleotide primers (one primer per reaction). There was 94% agreement between the ERIC and RAPD profiles in classifying sets of isolates as uniform versus closely related but not identical versus probably unrelated, indicating a high kappa statistic of 0.8942. Subtle differences in related ERIC or RAPD patterns likely reflect gene transfer between strains, recombination, and/or mutation, whereas markedly different patterns reflect infection by unrelated strains. At least half of infected shantytown residents seemed to carry more than one H. pylori strain, although in 19 of 31 persons, the strains were closely related. Three RAPD tests, each with a different primer, were needed to achieve the sensitivity of one ERIC test. ERIC-PCR constitutes a resource- and time-efficient method for H. pylori strain differentiation.

  12. Working with Working Families. ERIC Digest.

    Science.gov (United States)

    King, Margaret

    This ERIC Digest offers tips for understanding the needs, concerns, and feelings of employed parents, and discusses ways child caregivers can involve working parents in their children's lives while the children are enrolled in child care facilities. Competition with the caregiver, guilt over leaving children in the care of others, and little time…

  13. Manejo de riesgo (Risk Management). ERIC Digest.

    Science.gov (United States)

    Gaustad, Joan

    The ordinary conduct of school business is accompanied today by risks that were rare or unknown a few decades ago. This ERIC Digest in Spanish discusses how risk management, a concept long used by corporate decision makers, can help school boards and administrators conserve their districts' assets. Risk management is a coordinated effort to…

  14. Indexing. ERIC Processing Manual, Section VII.

    Science.gov (United States)

    Houston, Jim, Ed.

    Rules and guidelines are provided for subject indexing in the ERIC system. The principle of "subject access" is discussed with particular reference to "coordinate indexing," which involves designating subject content by unit terms (or tags) that may be put together or "coordinated" for subsequent retrieval. The nature…

  15. Eric Garcia: Warrior with a Pen

    Science.gov (United States)

    Briggs, Judith

    2013-01-01

    Eric Garcia's large-scale oil paintings, drawings, installations, prints, and political cartoons examine versions of American history that have been overlooked and whitewashed. Aware that dominant history reflects a strategy of power, Garcia embraces the confluence of history, culture, and politics to challenge historical mythologies and…

  16. Risk Management. ERIC Digest, Number 86.

    Science.gov (United States)

    Gaustad, Joan

    The ordinary conduct of school business is accompanied today by risks that were rare or unknown a few decades ago. This ERIC Digest discusses how risk management, a concept long used by corporate decision makers, can help school boards and administrators conserve their districts' assets. Risk management is a coordinated effort to protect an…

  17. Indicadores educacionales (Educational Indicators). ERIC Digest.

    Science.gov (United States)

    Lashway, Larry

    Educators use informal theories and assumptions to guide their actions, yet often fail to evaluate these beliefs. This ERIC Digest in Spanish examines the nature and purpose of educational-indicator systems and discusses the design of report cards by which schools can inform the public of their performance. An indicator is any statistic that casts…

  18. La Disciplina Positiva (Positive Discipline). ERIC Digest.

    Science.gov (United States)

    ERIC Clearinghouse on Elementary and Early Childhood Education, Urbana, IL.

    This ERIC Digest suggests methods and language that can be used in handling difficult, but common, situations involving young children. The digest explains 12 methods of disciplining children that promote children's self-worth. These methods are: (1) showing children that the reasons for their actions are understood; (2) stating reasons; (3)…

  19. Grammatical Change through Repetition.

    Science.gov (United States)

    Arevart, Supot

    1989-01-01

    The effect of repetition on grammatical change in an unrehearsed talk is examined based on a case study of a single learner. It was found that repetition allows for accuracy monitoring in that errors committed in repeated contexts undergo correction. Implications for teaching are discussed. (23 references) (LB)

  20. The Negative Repetition Effect

    Science.gov (United States)

    Mulligan, Neil W.; Peterson, Daniel J.

    2013-01-01

    A fundamental property of human memory is that repetition enhances memory. Peterson and Mulligan (2012) recently documented a surprising "negative repetition effect," in which participants who studied a list of cue-target pairs twice recalled fewer targets than a group who studied the pairs only once. Words within a pair rhymed, and…

  1. Roles of repetitive sequences

    Energy Technology Data Exchange (ETDEWEB)

    Bell, G.I.

    1991-12-31

    The DNA of higher eukaryotes contains many repetitive sequences. The study of repetitive sequences is important, not only because many have important biological function, but also because they provide information on genome organization, evolution and dynamics. In this paper, I will first discuss some generic effects that repetitive sequences will have upon genome dynamics and evolution. In particular, it will be shown that repetitive sequences foster recombination among, and turnover of, the elements of a genome. I will then consider some examples of repetitive sequences, notably minisatellite sequences and telomere sequences as examples of tandem repeats, without and with respectively known function, and Alu sequences as an example of interspersed repeats. Some other examples will also be considered in less detail.

  2. Roles of repetitive sequences

    Energy Technology Data Exchange (ETDEWEB)

    Bell, G.I.

    1991-12-31

    The DNA of higher eukaryotes contains many repetitive sequences. The study of repetitive sequences is important, not only because many have important biological function, but also because they provide information on genome organization, evolution and dynamics. In this paper, I will first discuss some generic effects that repetitive sequences will have upon genome dynamics and evolution. In particular, it will be shown that repetitive sequences foster recombination among, and turnover of, the elements of a genome. I will then consider some examples of repetitive sequences, notably minisatellite sequences and telomere sequences as examples of tandem repeats, without and with respectively known function, and Alu sequences as an example of interspersed repeats. Some other examples will also be considered in less detail.

  3. Civismo, República e manuais escolares

    Directory of Open Access Journals (Sweden)

    Cleber Santos Vieira

    2012-01-01

    Full Text Available O artigo analisa aspectos do civismo no alvorecer da República brasileira, com ênfase na pesquisa sobre dois livros escolares: História do Brasil ensinada pela biografia de seus heróis, de Silvio Romero (1890; e A História de São Paulo pela biografia de seus nomes mais notáveis (1895, publicado por Tancredo Amaral. Por um lado, trata-se de apresentar nuances da Historia Magistra Vitae presentes nestes objetos da cultura material. Por outro, de destacar em que medida suas histórias foram pontuadas por divergências em torno da questão educação ou instrução cívica pela afirmação de um civismo de coloração regional, bem como palco de conflitos entre protagonistas do republicanismo paulista.

  4. Repetition and Translation Shifts

    Directory of Open Access Journals (Sweden)

    Simon Zupan

    2006-06-01

    Full Text Available Repetition manifests itself in different ways and at different levels of the text. The first basic type of repetition involves complete recurrences; in which a particular textual feature repeats in its entirety. The second type involves partial recurrences; in which the second repetition of the same textual feature includes certain modifications to the first occurrence. In the article; repetitive patterns in Edgar Allan Poe’s short story “The Fall of the House of Usher” and its Slovene translation; “Konec Usherjeve hiše”; are compared. The author examines different kinds of repetitive patterns. Repetitions are compared at both the micro- and macrostructural levels. As detailed analyses have shown; considerable microstructural translation shifts occur in certain types of repetitive patterns. Since these are not only occasional; sporadic phenomena; but are of a relatively high frequency; they reduce the translated text’s potential for achieving some of the gothic effects. The macrostructural textual property particularly affected by these shifts is the narrator’s experience as described by the narrative; which suffers a reduction in intensity.

  5. Comparison of genetic variability between Czech and foreign isolates of phytopathogenic bacteria Clavibacter michiganensis subsp. sepedonicus by Rep-PCR technique.

    Science.gov (United States)

    Fousek, J; Mráz, I; Petrzik, K

    2002-01-01

    Repetitive-sequence-based polymerase chain reaction (Rep-PCR) method was used for analysis of genetic variability among bacterial populations from different world locations. Collection of 26 Czech and 13 foreign strains of Clavibacter michiganensis subsp. sepedonicus was amplified using BOX primer targeting to repetitive motif occurring in eubacterial genomes. Genetic fingerprints were visually compared and statistically evaluated by cluster analysis. Genetic similarity was estimated to be approximately 80% among all tested strains. Populations of these bacteria seem to be highly homogeneous; potential influence of geographic origin was not confirmed.

  6. Trialogue: Preparation, Repetition and...

    Science.gov (United States)

    Oberg, Antoinette; And Others

    1996-01-01

    This paper interrogates both curriculum theory and the limits and potentials of textual forms. A set of overlapping discourses (a trialogue) focuses on inquiring into the roles of obsession and repetition in creating deeply interpretive locations for understanding. (SM)

  7. Virginia Tech Distinguished Lecture Series: Eric Lyon

    OpenAIRE

    Lyon, Eric

    2015-01-01

    Associate Professor - Music Technology, Composition Eric Lyon is a composer and computer music researcher. Major areas of focus include computer chamber music, spatial orchestration, and articulated noise composition. Recent compositions include "Spirits", a 43-channel electroacoustic piano composition for the ZKM Kubus,"Noise Variations" for ensemble mise-en, and “The Book of Strange Positions” for the violin duo String Noise. Subject: Distinguished Lecture Series

  8. Pensar la república : revolución y positivismo en los orígenes de la tercera república francesa

    OpenAIRE

    López Alcañiz, Vladimir

    2005-01-01

    El artículo reflexiona entorno la idea de república durante la tercera república francesa. El artículo incluye una bibliografía comentada referente al legado de la Revolución y los orígenes de la República. L’article reflexiona entorn la idea de república durant la tercera república francesa. L’article inclou una bibliografia comentada referent al llegat de la Revolució i els orígens de la República. The article focus on republic thought during the third french republic. It includes a b...

  9. PepGMV Rep-Protein Expression in Mammalian Cells

    Science.gov (United States)

    Chapa-Oliver, Angela María; Mejía-Teniente, Laura; García-Gasca, Teresa; Guevara-Gonzalez, Ramon Gerardo; Torres-Pacheco, Irineo

    2012-01-01

    The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals. PMID:23170183

  10. República Saharaui: una nación cerca del olvido

    OpenAIRE

    Valdez Ugalde, María Cristina

    2010-01-01

    1. Párrafo de Inicio 2. Desarrollo a. República Saharaui o Sahara Occidental b. El gobierno de la República Saharaui c. La gente en la República Saharaui d. La ONU y la República Saharaui 3. Párrafo de Cierre 4. Referencias 5. Notas de enseñanza

  11. Cutting edge: natural DNA repetitive extragenic sequences from gram-negative pathogens strongly stimulate TLR9.

    Science.gov (United States)

    Magnusson, Mattias; Tobes, Raquel; Sancho, Jaime; Pareja, Eduardo

    2007-07-01

    Bacterial DNA exerts immunostimulatory effects on mammalian cells via the intracellular TLR9. Although broad analysis of TLR9-mediated immunostimulatory potential of synthetic oligonucleotides has been developed, which kinds of natural bacterial DNA sequences are responsible for immunostimulation are not known. This work provides evidence that the natural DNA sequences named repetitive extragenic palindromic (REPs) sequences present in Gram-negative bacteria are able to produce innate immune system stimulation via TLR9. A strong induction of IFN-alpha production by REPs from Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, and Neisseria meningitidis was detected in splenocytes from 129 mice. In addition, the involvement of TLR9 in immune stimulation by REPs was confirmed using B6.129P2-Tlr9(tm1Aki) knockout mice. Considering the involvement of TLRs in Gram-negative septic shock, it is conceivable that REPs play a role in its pathogenesis. This study highlights REPs as a potential novel target in septic shock treatment.

  12. Adult ESL Literacy Resources in the ERIC System.

    Science.gov (United States)

    Florez, MaryAnn Cunningham

    The guide provides a quick reference to the database, services, and additional resources of the ERIC System that may be of use in adult English-as-a-Second-Language (ESL) literacy work. It first explains the role of the National Clearinghouse for ESL Literacy Education, then describes the ERIC database and the kinds of adult ESL literacy materials…

  13. Skyview Foods: Eric's Real Dilemma with Channel Partners

    Science.gov (United States)

    Castleberry, Stephen B.

    2011-01-01

    As a marketing analyst, Eric is faced with several ethical dilemmas. When asked to engage in unethical and illegal behavior, Eric must decide whether to obey his boss and fudge the numbers (the counts of product purchased in order to get reimbursed more from the manufacturer during special sales) or take the ethical route. The case consists of two…

  14. Computer Cache: ERIC and the Library Media Specialist.

    Science.gov (United States)

    Lodish, Erica K.

    1986-01-01

    Description of how library media specialists can assist teachers and administrators in retrieving useful information by using ERIC highlights its organization, the types of materials available that are especially useful to classroom teachers, and ways that it can be accessed online. A list of ERIC Clearinghouses with addresses is included. (EM)

  15. Catalog of ERIC Clearinghouse Publications, 2000. A Catalog of Free and Low-Cost ERIC Clearinghouse Materials Currently in Print.

    Science.gov (United States)

    Goncalves, Silvia, Ed.

    This Catalog highlights more than 1,200 available education titles produced directly by the Educational Resources Information Center (ERIC) system. The titles are arranged by ERIC component and cover a broad range of subject areas related to education. The Catalog provides ordering information and prices (subject to change) for each title and…

  16. Differentiation Between Bacillus thuringiensis and Bacillus cereus by 16S rDNA-PCR and ERIC-PCR

    Institute of Scientific and Technical Information of China (English)

    LI Haitao; LIU Dongming; GAO Jiguo

    2011-01-01

    16S rDNA and ERIC (Enterobacteia Repetitive Intergenic Consensus Sequences) based on PCR method were tested for the effectiveness of the differentiation of B. thuringiensis and B. cereus. 16S rDNA-PCR primers were designed based on the sequence difference in variable regions of B. cereus 16S rDNA and B. thuringiensis 16S rDNA, 16S rDNA-PCR showed no obvious difference between B. cereus and B. thuringiensis. The only difference was that one 1600-bp amplificon could be obtained from all the three B. Cereus strains, and none amplificon from any B. thuringiensis strains. ERIC was optimized based on previous reports. The genonlic DNA was used for the template of ER1C-PCR, and the following DNA fingerprints were analyzed by the agarose gel electrophoresis. The results showed that DNA fingerprint of three B. thuringiensis strains had a unique amplicon less than 100-bp, while DNA fingerprint of three B. cereus" strains had none. Moreover, DNA fingerprint of B. cereus showed a 700-bp amplicon, but didn't have any DNA fingerprints ofB. thuringiensis genome. Therefore, ERIC-PCR technique should be able to be used for the differentiation of B. thuringiensis and B. cereus.

  17. Eric Hobsbawm: historia social e historia militante

    Directory of Open Access Journals (Sweden)

    Pablo A. Pozzi

    2007-07-01

    Full Text Available This piece is a brief and critical historiographical discussion ofthe contribution of the great English historian Eric Hobsbawm. Its main thesis is that Hobsbawm built his history on a particular relationship between history and society to develop a peculiar vision that articulated academic soundness with political advocacy. At the same time, he succeeded in making significant contributions to historical studies, politics, and even theoretical Marxism. In the end the fall of the USSR became a watershed that strongly influencedHobsbawm’s historical works.

  18. Specific functions of the Rep and Rep' proteins of porcine circovirus during copy-release and rolling-circle DNA replication

    Science.gov (United States)

    The roles of two porcine circovirus replication initiator proteins, Rep and Rep', in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replica...

  19. ERIC-PCR Genotyping of Some Campylobacter jejuni Isolates of Chicken and Human Origin in Egypt.

    Science.gov (United States)

    Ahmed, Heba A; El Hofy, Fatma I; Ammar, Ahmed M; Abd El Tawab, Ashraf A; Hefny, Ahmed A

    2015-12-01

    The public health importance of the genus Campylobacter is attributed to several species causing diarrhea in consumers. Poultry and their meat are considered the most important sources of human campylobacteriosis. In this study, 287 samples from chicken (131 cloacal swabs, 39 chicken skin, 78 chicken meat, and 39 cecal parts) obtained from retail outlets as well as 246 stool swabs from gastroenteritis patients were examined. A representative number of the biochemically identified Campylobacter jejuni isolates were identified by real-time PCR, confirming the identification of the isolates as C. jejuni. Genotyping of the examined isolates (n = 31) by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) revealed a high discriminatory index of ERIC-PCR (D = 0.948), dividing C. jejuni isolates of chicken and human origins into 18 profiles and four clusters. The 18 profiles obtained indicated the heterogeneity of C. jejuni. Dendrogram analysis showed that four clusters were generated; all human isolates fell into clusters I and III. These observations further support the existence of a genetic relationship between human and poultry isolates examined in the present study. In conclusion, the results obtained support the speculation that poultry and poultry meat have an important role as sources of infection in the acquisition of Campylobacter infection in humans.

  20. Molecular typing of uropathogenic E. coli strains by the ERIC-PCR method.

    Science.gov (United States)

    Ardakani, Maryam Afkhami; Ranjbar, Reza

    2016-04-01

    Escherichia coli (E. coli) is the most common cause of urinary infections in hospitals. The aim of this study was to evaluate the ERIC-PCR method for molecular typing of uropathogenic E. coli strains isolated from hospitalized patients. In a cross sectional study, 98 E. coli samples were collected from urine samples taken from patients admitted to Baqiyatallah Hospital from June 2014 to January 2015. The disk agar diffusion method was used to determine antibiotic sensitivity. DNA proliferation based on repetitive intergenic consensus was used to classify the E. coli strains. The products of proliferation were electrophoresed on 1.5% agarose gel, and their dendrograms were drawn. The data were analyzed by online Insillico software. The method used in this research proliferated numerous bands (4-17 bands), ranging from 100 to 3000 base pairs. The detected strains were classified into six clusters (E1-E6) with 70% similarity between them. In this study, uropathogenic E. coli strains belonged to different genotypic clusters. It was found that ERIC-PCR had good differentiation power for molecular typing of uropathogenic E. coli strains isolated from the patients in the study.

  1. Novel porcine repetitive elements

    Directory of Open Access Journals (Sweden)

    Nonneman Dan J

    2006-12-01

    Full Text Available Abstract Background Repetitive elements comprise ~45% of mammalian genomes and are increasingly known to impact genomic function by contributing to the genomic architecture, by direct regulation of gene expression and by affecting genomic size, diversity and evolution. The ubiquity and increasingly understood importance of repetitive elements contribute to the need to identify and annotate them. We set out to identify previously uncharacterized repetitive DNA in the porcine genome. Once found, we characterized the prevalence of these repeats in other mammals. Results We discovered 27 repetitive elements in 220 BACs covering 1% of the porcine genome (Comparative Vertebrate Sequencing Initiative; CVSI. These repeats varied in length from 55 to 1059 nucleotides. To estimate copy numbers, we went to an independent source of data, the BAC-end sequences (Wellcome Trust Sanger Institute, covering approximately 15% of the porcine genome. Copy numbers in BAC-ends were less than one hundred for 6 repeat elements, between 100 and 1000 for 16 and between 1,000 and 10,000 for 5. Several of the repeat elements were found in the bovine genome and we have identified two with orthologous sites, indicating that these elements were present in their common ancestor. None of the repeat elements were found in primate, rodent or dog genomes. We were unable to identify any of the replication machinery common to active transposable elements in these newly identified repeats. Conclusion The presence of both orthologous and non-orthologous sites indicates that some sites existed prior to speciation and some were generated later. The identification of low to moderate copy number repetitive DNA that is specific to artiodactyls will be critical in the assembly of livestock genomes and studies of comparative genomics.

  2. Identification and molecular epidemiology of dermatophyte isolates by repetitive-sequence-PCR-based DNA fingerprinting using the DiversiLab system in Turkey.

    Science.gov (United States)

    Koc, A Nedret; Atalay, Mustafa A; Inci, Melek; Sariguzel, Fatma M; Sav, Hafize

    2017-05-01

    Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep-PCR. In the identification of dermatophyte isolates, agreement between rep-PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A -D) which were determined by the use of rep-PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep-PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep-PCR. © 2017 Blackwell Verlag GmbH.

  3. A República Mundial de Otfried Höffe

    Directory of Open Access Journals (Sweden)

    Diego Carlos Zanella

    2015-03-01

    Full Text Available Esse texto tem por objetivo apresentar em linhas gerais a proposta de Otfried Höffe sobre a república mundial. Para tanto, iniciar-se-á com o ponto de partida de Höffe, a saber, o fenômeno da globalização, de onde são apontadas as principais atividades e problemas da república mundial. Em seguida, passa-se a analisar os dois níveis da proposta, o nível nacional e o nível internacional ou global da república mundial. Por fim, concluir-se-á com uma tentativa de comparação da proposta de Höffe com a ideia de uma federação de Estados livres apresentada por Immanuel Kant em À paz perpétua.

  4. 3D printing with RepRap cookbook

    CERN Document Server

    Salinas, Richard

    2014-01-01

    A systematic guide consisting of over 100 recipes which focus on helping you understand the process of 3D printing using RepRap machines. The book aims at providing professionals with a series of working recipes to help make their fuzzy notions into real, saleable projects/objects using 3D printing technology. This book is for novice designers and artists who own a RepRap-based 3D printer, have fundamental knowledge of its working, and who desire to gain better mastery of the printing process. For the more experienced user, it will provide a handy visual resource, with side-by-side comparisons

  5. Images en mouvement stockage, repérage, indexation

    CERN Document Server

    Turner, James

    1998-01-01

    L'avènement puis la fusion des nouveaux modes de communication que sont l'informatique, les télécommunications et l'audiovisuel ont mis à la portée de tous une grande quantité d'images fixes et en mouvement dont la conservation et le repérage risquent de prendre des proportions démesurées. Le présent ouvrage veut offrir aux responsables de collection des repères pour aborder la problématique de l'indexation des images et faciliter l'accès des usagers à ces images.

  6. Genetic diversity among Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis strains using repetitive element polymorphism-PCR.

    Science.gov (United States)

    Brumlik, Michael J; Bielawska-Drózd, Agata; Zakowska, Dorota; Liang, Xudong; Spalletta, Ronald A; Patra, Guy; Delvecchio, Vito G

    2004-01-01

    Repetitive element polymorphism-PCR (REP-PCR) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. Using the MB1 primer, REP-PCR fingerprints from 110 Bacillus strains within the "B. cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. All Bacillus anthracis strains tested were found to be monomorphic by fluorophore-enhanced REP-PCR (FERP) fingerprinting using the MB1 primer. In contrast, other non- B. anthracis isolates displayed a high degree of polymorphism. Dendrogramic analysis revealed that the non- B. anthracis strains possessing the Ba813 chromosomal marker were divided into two clusters. One of the clusters shared identity with the B. cereus strains examined.

  7. Benefits of Art and Music Education. ERIC/EECE Report.

    Science.gov (United States)

    Cesarone, Bernard

    1999-01-01

    Annotates 13 recent documents and journals from the ERIC database that discuss the benefits of art and music education to children's development and academic achievement. Includes art and music education Internet sites. (LBT)

  8. Communication in the Classroom: Research and Observation. ERIC Report.

    Science.gov (United States)

    Boileau, Don M.

    1981-01-01

    Presents an annotated bibliography of ERIC resources on the following topics: teacher communication; research on classroom interaction; using systematic observations to improve teaching; different systems of classroom observations; and research on classroom observation techniques. (PD)

  9. Eric Kandel: the future of memory.

    Science.gov (United States)

    Kandel, Erik

    2005-04-01

    Watching ice floes glide by on the Hudson River from Eric Kandel's office, one gets a sense of placid reflection tempered by constant action-an apt analogy for Kandel's ability to calmly manage several ongoing projects and commitments at once. In addition to his well-lauded, ongoing research at Columbia University Medical Center's New York State Psychiatric Institute, Kandel has written several books on neurobiology, behavior, and memory. In addition to being a Nobel Laureate Scientist, he is well-known as an editor of the seminal textbook Principles of Neural Science. He and his colleagues are in the midst of working on a new edition of Principles, and he is working on a scientific autobiography. MI sat down with Dr. Kandel and discussed with him a range of topics including childhood and early career influences, intramural research at the NIH, the HHMI, ethical considerations of altering memory and, of course, Aplysia.

  10. CAS Accelerator Physics held in Erice, Italy

    CERN Multimedia

    CERN Accelerator School

    2013-01-01

    The CERN Accelerator School (CAS) recently organised a specialised course on Superconductivity for Accelerators, held at the Ettore Majorana Foundation and Centre for Scientific Culture in Erice, Italy from 24 April-4 May, 2013.   Photo courtesy of Alessandro Noto, Ettore Majorana Foundation and Centre for Scientific Culture. Following a handful of summary lectures on accelerator physics and the fundamental processes of superconductivity, the course covered a wide range of topics related to superconductivity and highlighted the latest developments in the field. Realistic case studies and topical seminars completed the programme. The school was very successful with 94 participants representing 23 nationalities, coming from countries as far away as Belorussia, Canada, China, India, Japan and the United States (for the first time a young Ethiopian lady, studying in Germany, attended this course). The programme comprised 35 lectures, 3 seminars and 7 hours of case study. The case studies were p...

  11. Could the DiversiLab® semi-automated repetitive-sequence-based PCR be an acceptable technique for typing isolates of Pseudomonas aeruginosa? An answer from our experience and a review of the literature.

    Science.gov (United States)

    Brossier, Florence; Micaelo, Maïté; Luyt, Charles-Edouard; Lu, Qin; Chastre, Jean; Arbelot, Charlotte; Trouillet, Jean-Louis; Combes, Alain; Rouby, Jean-Jacques; Jarlier, Vincent; Aubry, Alexandra

    2015-12-01

    Recently the DiversiLab® (DL) system (bioMérieux) was developed as an automated platform that uses repetitive element polymerase chain reaction (rep-PCR) technology for standardized, reproducible DNA fingerprinting of bacteria. The purpose of this study was to evaluate the usefulness of DL rep-PCR for typing Pseudomonas aeruginosa isolates. The performance of DL rep-PCR was compared with that of pulsed-field gel electrophoresis (PFGE) in a prospective multicenter study of patients with ventilator-associated pneumonia due to P. aeruginosa, conducted in 3 intensive care units over a 31-month period. In total, 203 P. aeruginosa isolates from 66 patients, from whom at least 2 consecutive respiratory samples each were collected more than 48 h apart, were typed using DL rep-PCR. Forty isolates (corresponding to 20 patients) were also typed using PFGE of SpeI-digested DNA. The typeability was 100% with DL rep-PCR and 95% with PFGE. The discriminatory power was close for DL rep-PCR and for PFGE (Simpson's diversity indices of 0.901 and 0.947, respectively). Insufficient agreement between DL rep-PCR and PFGE typing results was observed for the 40 selected isolates (adjusted Rand coefficient of 0.419), mostly due to isolates of the same DL rep-PCR type but of different PFGE types (adjusted Wallace coefficients of 0.306 for DL rep-PCR with PFGE, and of 0.667 for PFGE with DL rep-PCR). Considered together with published data, DL rep-PCR results should be interpreted with caution for the investigation of outbreaks caused by P. aeruginosa and evaluated in conjunction with epidemiological data.

  12. Eric Soovere fotodest sõjafotograafia kontektis / Ahto Sooaru

    Index Scriptorium Estoniae

    Sooaru, Ahto

    2011-01-01

    Püütakse selgitada Eric Soovere piltide ja teksti tausta põgenemisel kodumaalt, näidatakse tema töö tähtsust Eesti visuaalkultuuris ja käsitletakse ka sõjafotograafia ajalugu ning sõjafotograafia peegeldusi kunstis. Praktilise tööna sündis DVD, kuhu on koondatud Eric Soovere päevik ja fotomaterjal kaasaegse formaadina

  13. Eric Soovere fotodest sõjafotograafia kontektis / Ahto Sooaru

    Index Scriptorium Estoniae

    Sooaru, Ahto

    2011-01-01

    Püütakse selgitada Eric Soovere piltide ja teksti tausta põgenemisel kodumaalt, näidatakse tema töö tähtsust Eesti visuaalkultuuris ja käsitletakse ka sõjafotograafia ajalugu ning sõjafotograafia peegeldusi kunstis. Praktilise tööna sündis DVD, kuhu on koondatud Eric Soovere päevik ja fotomaterjal kaasaegse formaadina

  14. Las Garrapatas de la República de Colombia

    Directory of Open Access Journals (Sweden)

    Osorno Mesa Ernesto

    1942-04-01

    Full Text Available El presente estudio comprende las especies de garrapatas colombianas conocidas, como también las de la República de Panamá. En las claves incluyo las especies descritas y halladas en Colombia. Las referencias fueron tomadas de las descripciones originales y de todas las publicaciones sobre garrapatas de los dos países.

  15. El movimiento no parlamentario en la Segunda República

    Directory of Open Access Journals (Sweden)

    Víctor ALBA

    2010-02-01

    Full Text Available Cuando se escribe sobre la Segunda República española se hace frecuente referencia a las derechas, los partidos republicanos y los socialistas. Menos frecuentes son las alusiones al movimiento narcosindicalista y casi nulas las que se relacionan con los partidos marxistas menores.

  16. Rehabilitation Education Program for Stroke (REPS): Learning and Practice Outcomes

    Science.gov (United States)

    McEwen, Sara; Szurek, Kristina; Polatajko, Helene J.; Rappolt, Susan

    2005-01-01

    Introduction: New research knowledge acquired from Web-based sources may have a better chance of being translated into practice when accompanied by additional educational strategies. This study was undertaken to investigate that hypothesis. Methods: The Rehabilitation Education Program for Stroke (REPS) combines a self-directed online learning…

  17. Las garrapatas de la república de colombia

    OpenAIRE

    Osorno Mesa Ernesto

    2012-01-01

    El presente estudio comprende las especies de garrapatas colombianas conocidas, como también las de la República de Panamá. En las claves incluyo las especies descritas y halladas en Colombia. Las referencias fueron tomadas de las descripciones originales y de todas las publicaciones sobre garrapatas de los dos países.

  18. MIMICRY, DIFFERENCE AND REPETITION

    Directory of Open Access Journals (Sweden)

    Marcelo Mendes de Souza

    2008-07-01

    Full Text Available This article addresses Homi K. Bhabha’s concept of mimicry in a broader context, other than that of cultural studies and post-colonial studies, bringing together other concepts, such as that of Gilles Deleuze in Difference and repetition, among other texts, and other names, such as Silviano Santiago, Jorge Luís Borges, Franz Kafka and Giorgio Agamben. As a partial conclusion, the article intends to oppose Bhabha’s freudian-marxist view to Five propositions on Psychoanalysis (1973, Gilles Deleuze’s text about Psychoanalysis published right after his book The Anti-Oedipus.

  19. Use of long-range repetitive element polymorphism-PCR to differentiate Bacillus anthracis strains.

    Science.gov (United States)

    Brumlik, M J; Szymajda, U; Zakowska, D; Liang, X; Redkar, R J; Patra, G; Del Vecchio, V G

    2001-07-01

    The genome of Bacillus anthracis is extremely monomorphic, and thus individual strains have often proven to be recalcitrant to differentiation at the molecular level. Long-range repetitive element polymorphism-PCR (LR REP-PCR) was used to differentiate various B. anthracis strains. A single PCR primer derived from a repetitive DNA element was able to amplify variable segments of a bacterial genome as large as 10 kb. We were able to characterize five genetically distinct groups by examining 105 B. anthracis strains of diverse geographical origins. All B. anthracis strains produced fingerprints comprising seven to eight bands, referred to as "skeleton" bands, while one to three "diagnostic" bands differentiated between B. anthracis strains. LR REP-PCR fingerprints of B. anthracis strains showed very little in common with those of other closely related species such as B. cereus, B. thuringiensis, and B. mycoides, suggesting relative heterogeneity among the non-B. anthracis strains. Fingerprints from transitional non-B. anthracis strains, which possessed the B. anthracis chromosomal marker Ba813, scarcely resembled those observed for any of the five distinct B. anthracis groups that we have identified. The LR REP-PCR method described in this report provides a simple means of differentiating B. anthracis strains.

  20. Overview of RepLab 2013: Evaluating online reputation monitoring systems

    NARCIS (Netherlands)

    Amigó, E.; Carrillo de Albornoz, J.; Chugur, I.; Corujo, A.; Gonzalo, J.; Martín, T.; Meij, E.; de Rijke, M.; Spina, D.

    2013-01-01

    This paper summarizes the goals, organization, and results of the second RepLab competitive evaluation campaign for Online Reputation Management Systems (RepLab 2013). RepLab focused on the process of monitoring the reputation of companies and individuals, and asked participant systems to annotate

  1. Overview of RepLab 2012: Evaluating Online Reputation Management Systems

    NARCIS (Netherlands)

    Amigó, E.; Corujo, A.; Gonzalo, J.; Meij, E.; de Rijke, M.

    2012-01-01

    This paper summarizes the goals, organization and results of the first RepLab competitive evaluation campaign for Online Reputation Management Systems (RepLab 2012). RepLab focused on the reputation of companies, and asked participant systems to annotate different types of information on tweets

  2. Specific functions of the Rep and Rep׳ proteins of porcine circovirus during copy-release and rolling-circle DNA replication.

    Science.gov (United States)

    Cheung, Andrew K

    2015-07-01

    The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates the single-stranded circular (ssc) genome from the displaced DNA strand. In the process, a minus-genome primer (MGP) necessary for complementary-strand synthesis, from ssc to ccc, is synthesized. Rep׳ cleaves the growing nascent-strand to regenerate the parent ccc molecule. In the process, a Rep׳-DNA hybrid containing the right palindromic sequence (at the origin of DNA replication) is generated. Analysis of the virus particle showed that it is composed of four components: ssc, MGP, capsid protein and a novel Rep-related protein (designated Protein-3).

  3. Gold-Collar Workers. ERIC Digest.

    Science.gov (United States)

    Wonacott, Michael E.

    The gold-collar worker has problem-solving abilities, creativity, talent, and intelligence; performs non-repetitive and complex work difficult to evaluate; and prefers self management. Gold-collar information technology workers learn continually from experience; recognize the synergy of teams; can demonstrate leadership; and are strategic thinkers…

  4. The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic reticulum membrane for degradation of misfolded proteins

    DEFF Research Database (Denmark)

    Madsen, Louise; Kriegenburg, Franziska; Lages Lino Vala, Andrea;

    2011-01-01

    protein named Rep8 or Ubxd6 as a new cofactor of p97. Mouse Rep8 is highly tissue-specific and abundant in gonads. In testes, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Rep8 associates directly with p97 via its UBX domain. We show that Rep8...

  5. Repetition in Waiting for Godot

    Institute of Scientific and Technical Information of China (English)

    李想; 魏妍

    2015-01-01

    Waiting for Godot is one of the most famous plays written by Samuel Barclay Beckett, and also is the founding work of“Theatre of the Absurd”. In the drama, repetitive phenomena shed light on the whole construction considerably. All the charac-ters were helpless and unthinking. Their dialogues were simple, nonsense and repetitive. Two scenes were cyclical. Repetition was used subtly in order to express the theme of the play, showing mental crisis after depravation of WWII.

  6. ERIC Abstracts: A Collection of ERIC Document Resumes on the Use of Futurism in Educational Planning. ERIC Abstracts Series, Number Thirty.

    Science.gov (United States)

    American Association of School Administrators, Washington, DC. National Academy for School Executives.

    Futurism is a process of speculation on alternative possibilities for the future. Such a process leads to a clearer conception of future needs and resources. Applied to education, futurism can aid in longrange planning on a local, district, State, or national level. ERIC abstracts on the application of futurism to educational planning, announced…

  7. Saladusi mäletavad pildid : järelvaade Eric Soovere fotograafilisele dokumentalistikale / Peeter Linnap

    Index Scriptorium Estoniae

    Linnap, Peeter, 1960-

    2010-01-01

    Eric Sooverest ja tema fotoloomingust. Eric Soovere ja Johannes Pääsukese loomingu võrdlus. Katkendeid Eric Soovere raamatust "Käru ja kaameraga : pilte ja päevikulehti põgenemisteelt 1944-1949". Pikemalt emigreerumisest Ameerikasse

  8. A república paraense em festa (1890-1911)

    OpenAIRE

    MOURA, Daniella de Almeida

    2008-01-01

    A proposta desta dissertação é discutir a formação do poder simbólico da República Paraense nos seus primeiros anos (1890-1911), observando eventos como a mudança dos nomes de algumas ruas em homenagem ao novo regime, assim como a formulação do calendário republicano, com destaque para os festejos republicanos de 15 e 16 de novembro, datas da Proclamação da República e sua Aclamação no Pará, com o objetivo de analisar seus significados, organização e, principalmente, o entendimento da idéia d...

  9. Identification of Tilletia species using rep-PCR fingerprinting technique

    Directory of Open Access Journals (Sweden)

    Župunski Vesna

    2011-01-01

    Full Text Available Analyzing 167 non-processed seed samples of wheat, it was found that 145 samples (86.8 % were contaminated with Tilletia species, while 22 (13.2 % samples were not contaminated. By using rep-PCR fingerprinting technique, it was found that DNA isolates of T. tritici originated from Serbian wheat samples had 80 % similarity with positive control for T. tritici. One isolate shared similarity of 60% with T. tritici, T. controversa and T. laevis. It was supposed that this isolate belongs to T. bromi. Isolate of T. laevis shared a similarity of 70 % with isolates of T. tritici and T. controversa, while T. walkeri was more than 10 % similar with T. tritici, T. controversa and T. laevis. Although T. controversa and T. tritici had high percent of genetic similarity, they were clustered separately. Our results suggest that rep-PCR fingerprinting could be a useful tool for monitoring presence of morphologically similar Tilletia species in wheat production areas.

  10. Arduino & RepRap - Creating Wealth by Giving it Away

    DEFF Research Database (Denmark)

    2011-01-01

    , growth in wealth is longevity and prosperity. Is it possible to grow wealth independently of money? This talk will be from Adrian Bowyer - creator of RepRap, the open-source replicating 3D printer - and from David Cuartielles - creator of Arduino, the open-source microcontroller. Both projects have...... founded significant and growing industries - and hence significant and growing wealth - by giving away all the data required to build RepRaps and Arduinos completely free. They have also short-circuited most conventional industrial infrastructure by placing the ability to create wealth directly...... in the hands of private individuals. The presenters contend that this is the way of the future: companies, and - more importantly - those private individuals will be giving away their primary products and making a living on the sideline activities that such donations attract. Software has been heading...

  11. ERIC-PCR identification of the spread of airborne Escherichia coli in pig houses.

    Science.gov (United States)

    Yuan, W; Chai, T J; Miao, Z M

    2010-02-15

    To understand the spread of microbial aerosols in pig houses, with Escherichia coli (E. coli) as indicator, the airborne E. coli in 4 pig houses and their surroundings at different points 10, 50m upwind and 10, 50, 100, 200 and 400m downwind respectively from the pig houses were collected, and the concentrations were calculated at each sampling point. Furthermore, the feces of pigs were collected to separate E. coli. The ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction) technology was used to amplify the isolated E. coli DNA samples, then the amplified results were analyzed by NTSYS-pc (Version 2.10) to identify the similarity of isolated E. coli. The results showed that the airborne E. coli concentrations in indoor air of the 4 pig houses (21-35CFUm(-)(3) air) were much higher than those in upwind and downwind air (P0.05) at downwind distances. The ERIC-PCR results also showed that 52.4% of the fecal E. coli (four houses being respectively 2/4, 50%; 2/4, 50%; 3/6, 50%; 4/7, 57.1%) were identical to the indoor airborne E. coli isolates, and there was more than 90% similarity between the majority of E. coli (50%, 21/42) isolated from downwind air at 10, 50, 100 and 200m and those from indoor air or feces. It could be concluded that the aerosols in pig houses can spread to the surroundings, and thus effective measures should be taken to control and minimize the spread of microbial aerosols.

  12. Understanding maximal repetitions in strings

    CERN Document Server

    Crochemore, Maxime

    2008-01-01

    The cornerstone of any algorithm computing all repetitions in a string of length n in O(n) time is the fact that the number of runs (or maximal repetitions) is O(n). We give a simple proof of this result. As a consequence of our approach, the stronger result concerning the linearity of the sum of exponents of all runs follows easily.

  13. Perceptual Repetition Blindness Effects

    Science.gov (United States)

    Hochhaus, Larry; Johnston, James C.; Null, Cynthia H. (Technical Monitor)

    1994-01-01

    The phenomenon of repetition blindness (RB) may reveal a new limitation on human perceptual processing. Recently, however, researchers have attributed RB to post-perceptual processes such as memory retrieval and/or reporting biases. The standard rapid serial visual presentation (RSVP) paradigm used in most RB studies is, indeed, open to such objections. Here we investigate RB using a "single-frame" paradigm introduced by Johnston and Hale (1984) in which memory demands are minimal. Subjects made only a single judgement about whether one masked target word was the same or different than a post-target probe. Confidence ratings permitted use of signal detection methods to assess sensitivity and bias effects. In the critical condition for RB a precue of the post-target word was provided prior to the target stimulus (identity precue), so that the required judgement amounted to whether the target did or did not repeat the precue word. In control treatments, the precue was either an unrelated word or a dummy.

  14. Quality of Researchers' Searches of the ERIC Database

    Directory of Open Access Journals (Sweden)

    Scott Hertzberg

    1999-08-01

    Full Text Available During the last ten years, end-users of electronic databases have become progressively less dependent on librarians and other intermediaries. This is certainly the case with the Educational Resources Information Center (ERIC Database, a resource once accessed by passing a paper query form to a librarian and now increasingly searched directly by end-users. This article empirically examines the search strategies currently being used by researchers and other groups. College professors and educational researchers appear to be doing a better job searching the database than other ERIC patrons. However, the study suggests that most end-users should be using much better search strategies.

  15. Fractal dimensions the digital art of Eric Hammel

    CERN Document Server

    Hammel, Eric

    2014-01-01

    The concept behind fractal geometry is extremely difficult to explain . . . but easy to see and enjoy. Eric Hammel, a professional author of military history books, is unable to explain fractals in a way that will be clear to anyone else, but most mathematicians can't explain fractals in language most people can understand. The simplest explanation is that fractals are graphic representations of high-order mathematical formulas that repeat patterns to infinity.Don't get hung up on the math. It's really all in the seeing. Like Volume 1 of Eric Hammel's Fractal Dimensions, Volume 2 is filled wit

  16. Fractal dimensions the digital art of Eric Hammel

    CERN Document Server

    Hammel, Eric

    2014-01-01

    The concept behind fractal geometry is extremely difficult to explain . . . but easy to see and enjoy. Eric Hammel, a professional author of military history books, is unable to explain fractals in a way that will be clear to anyone else, but most mathematicians can't explain fractals in language most people can understand. The simplest explanation is that fractals are graphic representations of high-order mathematical formulas that repeat patterns to infinity.Don't get hung up on the math. It's really all in the seeing. Like Volumes 1, 2, and 3 of Eric Hammel's Fractal Dimensions, Volume 4 is

  17. Fractal dimensions the digital art of Eric Hammel

    CERN Document Server

    Hammel, Eric

    2014-01-01

    The concept behind fractal geometry is extremely difficult to explain . . . but easy to see and enjoy. Eric Hammel, a professional author of military history books, is unable to explain fractals in a way that will be clear to anyone else, but most mathematicians can't explain fractals in language most people can understand. The simplest explanation is that fractals are graphic representations of high-order mathematical formulas that repeat patterns to infinity.Don't get hung up on the math. It's really all in the seeing. Like Volumes 1 and 2 of Eric Hammel's Fractal Dimensions, Volume 3 is fil

  18. Novel Mutant AAV2 Rep Proteins Support AAV2 Replication without Blocking HSV-1 Helpervirus Replication

    Science.gov (United States)

    Seyffert, Michael; Glauser, Daniel L.; Schraner, Elisabeth M.; de Oliveira, Anna-Paula; Mansilla-Soto, Jorge; Vogt, Bernd; Büning, Hildegard; Linden, R. Michael; Ackermann, Mathias; Fraefel, Cornel

    2017-01-01

    As their names imply, parvoviruses of the genus Dependovirus rely for their efficient replication on the concurrent presence of a helpervirus, such as herpesvirus, adenovirus, or papilloma virus. Adeno-associated virus 2 (AAV2) is such an example, which in turn can efficiently inhibit the replication of each helpervirus by distinct mechanisms. In a previous study we have shown that expression of the AAV2 rep gene is not compatible with efficient replication of herpes simplex virus 1 (HSV-1). In particular, the combined DNA-binding and ATPase/helicase activities of the Rep68/78 proteins have been shown to exert opposite effects on the replication of AAV2 and HSV-1. While essential for AAV2 DNA replication these protein activities account for the Rep-mediated inhibition of HSV-1 replication. Here, we describe a novel Rep mutant (Rep-D371Y), which displayed an unexpected phenotype. Rep-D371Y did not block HSV-1 replication, but still supported efficient AAV2 replication, at least when a double-stranded AAV2 genome template was used. We also found that the capacity of Rep-D371Y to induce apoptosis and a Rep-specific DNA damage response was significantly reduced compared to wild-type Rep. These findings suggest that AAV2 Rep-helicase subdomains exert diverging activities, which contribute to distinct steps of the AAV2 life cycle. More important, the novel AAV2 mutant Rep-D371Y may allow deciphering yet unsolved activities of the AAV2 Rep proteins such as DNA second-strand synthesis, genomic integration or packaging, which all involve the Rep-helicase activity. PMID:28125695

  19. Entrevista com Eric McLuhan

    Directory of Open Access Journals (Sweden)

    McLuhan, Eric

    2011-01-01

    Full Text Available Marshall McLuhan faria 100 anos em 2011. Para marcar esta data, que já provocou uma recente revisitação à obra do teórico, o XI Seminário Internacional da Comunicação, promovido pela PUCRS, destacou temas como as relações do homem com as tecnologias e os efeitos psicológicos das mídias para nortear às discussões dos três dias de evento. Mas se engana que acredita que entender a obra mcluhiana é tarefa fácil. Mesmo tendo se dedicado com afinco a explicar metáforas como ‘o meio é a mensagem’ e ‘aldeia global’, McLuhan nunca chegou a ser completamente entendido. Ou as pessoas não sedavam ao trabalho de entendê-lo, ou, simplesmente, não conseguiam enxergar as mudanças que estavam acontecendo com a mesma clareza que o pesquisador. Numa tentativa não só de entender a obra do ‘profeta da globalização’, mas de ampliar os entendimentos sobre as tecnologias e as extensões do homem, Eric McLuhan, filho do teórico, participou como palestrante do Seminário para falar, entre outros assuntos, da percepção acadêmica quanto a obra de seu pai, além de introduzir novas ideias sobre televisão, globalização e ecologia

  20. Monitoring transmission routes of Listeria spp. in smoked salmon production with repetitive element sequence-based PCR techniques.

    Science.gov (United States)

    Zunabovic, M; Domig, K J; Pichler, I; Kneifel, W

    2012-03-01

    Various techniques have been used for tracing the transmission routes of Listeria species and for the assessment of hygiene standards in food processing plants. The potential of repetitive element sequence-based PCR (Rep-PCR) methods (GTG₅ and REPI + II) for the typing of Listeria isolates (n = 116), including Listeria monocytogenes (n = 46), was evaluated in a particular situation arising from the relocation of a company producing cold-smoked salmon. Pulsed-field gel electrophoresis (PFGE) using three restriction enzymes (ApaI, AscI, and SmaI) was used for comparison. Identical transmission scenarios among two companies could be identified by cluster analysis of L. monocytogenes isolates that were indistinguishable by both Rep-PCR and PFGE. The calculated diversity index (DI) indicates that Rep-PCR subtyping of Listeria species with primer sets GTG₅ and REPI + II has a lower discrimination power than does PFGE. When concatenated Rep-PCR cluster analysis was used, the DI increased from 0.934 (REPI + II) and 0.923 (GTG₅) to 0.956. The discrimination power of this method was similar to that of PFGE typing based on restriction enzyme Apa I (DI = 0.955). Listeria welshimeri may be useful as an indicator for monitoring smoked salmon processing environments. Rep-PCR meets the expectations of a reasonable, fast, and low-cost molecular subtyping method for the routine monitoring of Listeria species. The discriminatory power as characterized by the DI sufficiently quantifies the probability of unrelated isolates being characterized as different subtypes. Therefore, Rep-PCR typing based on two primer systems (GTG₅ and REPI + II) may be a useful tool for monitoring industrial hygiene.

  1. Molecular characterization of Salmonella enterica serotype Enteritidis isolates from food and human samples by serotyping, antimicrobial resistance, plasmid profiling, (GTG5-PCR and ERIC-PCR

    Directory of Open Access Journals (Sweden)

    F. Fardsanei

    2016-11-01

    Full Text Available In recent years, Salmonella enterica serovar Enteritidis has been a primary cause of human salmonellosis in many countries. The major objective of this study was to investigate genetic diversity among Salmonella Enteritidis strains from different origins (food and human by Enterobacterial Repetitive Intergenic Consensus (ERIC -PCR, as well as to assess their plasmid profiling and antimicrobial resistance. A total of 30 Salmonella Enteritidis isolates, 15 from food samples (chicken, lamb, beef and duck meats and 15 from clinical samples were collected in Tehran. Identification of isolates as Salmonella was confirmed by using conventional standard biochemical and serological tests. Multiplex-PCR was used for serotyping of isolates to identify Salmonella Enteritidis. Antimicrobial susceptibility testing to 16 agents founds drug resistance patterns among Salmonella Enteritidis isolates. No resistance was observed to cephalexin, ceftriaxone, ceftazidime and cefotaxime, ciprofloxacin, imipenem or meropenem, chloramphenicol and gentamicin. The highest resistance (96.7% was observed to nitrofurantoin. Seven plasmid profiles (P1–P7 were detected, and a 68-kb plasmid was found in all isolates. Two different primers; ERIC and (GTG5 were used for genotyping, which each produced four profiles. The majority of clinical and food isolates fell into two separate common types (CTs with a similar percentage of 95% by ERIC-PCR. Using primer (GTG5, 29 isolates incorporated in three CTs with 70% of isolates showing a single banding pattern. Limited genetic diversity among human and food isolates of Salmonella Enteritidis may indicate that contaminated foods were possibly the source of human salmonellosis. These results confirmed that ERIC-PCR genotyping has limited discriminatory power for Salmonella Enteritidis of different origin.

  2. ERIC-PCR fingerprinting-based community DNA hybridization to pinpoint genome-specific fragments as molecular markers to identify and track populations common to healthy human guts.

    Science.gov (United States)

    Wei, Guifang; Pan, Li; Du, Huimin; Chen, Junyi; Zhao, Liping

    2004-10-01

    Bacterial populations common to healthy human guts may play important roles in human health. A new strategy for discovering genomic sequences as markers for these bacteria was developed using Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR fingerprinting. Structural features within microbial communities are compared with ERIC-PCR followed by DNA hybridization to identify genomic fragments shared by samples from healthy human individuals. ERIC-PCR profiles of fecal samples from 12 diseased or healthy human and piglet subjects demonstrated stable, unique banding patterns for each individual tested. Sequence homology of DNA fragments in bands of identical size was examined between samples by hybridization under high stringency conditions with DIG-labeled ERIC-PCR products derived from the fecal sample of one healthy child. Comparative analysis of the hybridization profiles with the original agarose fingerprints identified three predominant bands as signatures for populations associated with healthy human guts with sizes of 500, 800 and 1000 bp. Clone library profiling of the three bands produced 17 genome fragments, three of which showed high similarity only with regions of the Bacteroides thetaiotaomicron genome, while the remainder were orphan sequences. Association of these sequences with healthy guts was validated by sequence-selective PCR experiments, which showed that a single fragment was present in all 32 healthy humans and 13 healthy piglets tested. Two fragments were present in the healthy human group and in 18 children with non-infectious diarrhea but not in eight children with infectious diarrhea. Genome fragments identified with this novel strategy may be used as genome-specific markers for dynamic monitoring and sequence-guided isolation of functionally important bacterial populations in complex communities such as human gut microflora.

  3. Genotypic Characterization of Escherichia coli O157:H7 Isolates from Different Sources in the North-West Province, South Africa, Using Enterobacterial Repetitive Intergenic Consensus PCR Analysis

    Directory of Open Access Journals (Sweden)

    Collins Njie Ateba

    2014-05-01

    Full Text Available In many developing countries, proper hygiene is not strictly implemented when animals are slaughtered and meat products become contaminated. Contaminated meat may contain Escherichia coli (E. coli O157:H7 that could cause diseases in humans if these food products are consumed undercooked. In the present study, a total of 94 confirmed E. coli O157:H7 isolates were subjected to the enterobacterial repetitive intergenic consensus (ERIC polymerase chain reaction (PCR typing to generate genetic fingerprints. The ERIC fragments were resolved by electrophoresis on 2% (w/v agarose gels. The presence, absence and intensity of band data were obtained, exported to Microsoft Excel (Microsoft Office 2003 and used to generate a data matrix. The unweighted pair group method with arithmetic mean (UPGMA and complete linkage algorithms were used to analyze the percentage of similarity and matrix data. Relationships between the various profiles and/or lanes were expressed as dendrograms. Data from groups of related lanes were compiled and reported on cluster tables. ERIC fragments ranged from one to 15 per isolate, and their sizes varied from 0.25 to 0.771 kb. A large proportion of the isolates produced an ERIC banding pattern with three duplets ranging in sizes from 0.408 to 0.628 kb. Eight major clusters (I–VIII were identified. Overall, the remarkable similarities (72% to 91% between the ERIC profiles for the isolate from animal species and their corresponding food products indicated some form of contamination, which may not exclude those at the level of the abattoirs. These results reveal that ERIC PCR analysis can be reliable in comparing the genetic profiles of E. coli O157:H7 from different sources in the North-West Province of South Africa.

  4. Advisory Committees. The Best of ERIC, Number 30.

    Science.gov (United States)

    ERIC Clearinghouse on Educational Management, Eugene, OR.

    This annotated bibliography contains selected sources on advisory committees indexed in the ERIC system. The 11 documents and journal articles deal with various aspects of this topic, including the utilization of advisory committees for vocational and occupational education, the role of the school principal as committee leader, and how to obtain…

  5. Drug Testing. ERIC Digest Series Number EA 35.

    Science.gov (United States)

    Klauke, Amy

    The issue of drug testing is the focus of this ERIC Digest. Several aspects of drug testing discussed in question-and-answer format: (1) What is the current status of drug use in the schools? (2) What legal questions arise when schools consider drug testing? (3) How might drug testing be applied in a fair, economical, and legally safe manner? (4)…

  6. Mastery Learning and Mastery Testing: An Annotated ERIC Bibliography.

    Science.gov (United States)

    Wildemuth, Barbara M., Comp.

    This 136-item annotated bibliography on mastery learning and mastery testing is the result of a computer search of the ERIC data base in February 1977. All entries are listed alphabetically by author. An abstract or annotation is provided for each entry. A subject index is included reflecting the major emphasis of each citation. (RC)

  7. Integrating 21st Century Access to ERIC Services and Resources.

    Science.gov (United States)

    Sutton, Stuart A.

    2001-01-01

    Explores the development of the ERIC (Educational Resources Information Center) dataset and the relationship between that dataset and the disparate services that have emerged as a result of the Internet and the Web. Discusses the technical infrastructure; database content; services and resources; creating a unified knowledge base; and metadata…

  8. Information Technology in Education: The Best of ERIC.

    Science.gov (United States)

    Ely, Donald P.

    This publication highlights 48 recent ERIC listings which help to explain the variety of emerging technologies for the delivery of information in educational settings. Specific technologies addressed include cable television, electronic mail, satellite communication, teleconferencing, videodisc, and videotex. Entries were selected for inclusion…

  9. Constructivist Views of Learning in Science and Mathematics. ERIC Digest.

    Science.gov (United States)

    Ishii, Drew K.

    Many educators may or may not be familiar with the term constructivism, but probably recognize it as something to do with learning. The main tenet of constructivist learning is that people construct their own understanding of the world and in turn, their own knowledge. This ERIC Digest examines the constructivist view of learning and how it…

  10. The Making of a Missionary: Eric Ashby and Technology.

    Science.gov (United States)

    Silver, Harold

    2002-01-01

    Examines the work of Eric Ashby, a missionary of the technology age in Great Britain, an analyst of status quo and change proponents of higher education,; and an implementer of technology curricula. He warned in the 1950s that if Western academies did not adapt to/with technology, they would not survive. (KDR)

  11. The Industrial Revolution: An ERIC/ChESS Sample.

    Science.gov (United States)

    Pinhey, Laura A.

    2000-01-01

    Provides a list, from the ERIC database, of teaching materials and background information on the Industrial Revolution. Specific topics include life in Lowell (Massachusetts), the global impact of the Industrial Revolution, and England's Industrial Revolution. Offers directions for obtaining the full text of these materials. (CMK)

  12. Sport in History: An ERIC/ChESS Sample.

    Science.gov (United States)

    Schlene, Vickie J.

    1992-01-01

    Presents a sampling of eight entries from the ERIC database that deal with the history of sport. Includes works discussing baseball as a representation of societal value, lesson plans, and works dealing with race and cultural themes. Provides instructions for obtaining the documents listed. (DK)

  13. Consejeria de los directores (Principal Mentoring). ERIC Digest.

    Science.gov (United States)

    Malone, Robert J.

    To help new principals succeed, school districts are capitalizing on senior administrators' expertise by adding mentor programs to the practical training programs for beginning principals. This ERIC Digest in Spanish examines the nature of mentorships and discusses how they can prepare principals for the next stage of their careers. Although…

  14. Capital Outlay: A Critical Concern in Rural Education. ERIC Digest.

    Science.gov (United States)

    Hunter, James; Howley, Craig B.

    This digest, which is based primarily on the 1989 ERIC/CRESS monograph entitled "Achievement of Equity in Capital Outlay Financing: A Policy Analysis for the States," by D. Thompson G. Stewart, D. Honeyman, and R. Wood, addresses possible solutions to the emerging problem of capital outlay financing, with special attention to facilities…

  15. DSM-5 and ADHD - an interview with Eric Taylor.

    Science.gov (United States)

    Taylor, Eric

    2013-09-12

    In this podcast we talk to Prof Eric Taylor about the changes to the diagnosis of Attention Deficit Hyperactivity Disorder (ADHD) in DSM-5 and how these changes will affect clinical practice. The podcast for this interview is available at: http://www.biomedcentral.com/sites/2999/download/Taylor.mp3.

  16. Indexing for ERIC. Volume 3, Lessons 3 and 4.

    Science.gov (United States)

    Langridge, D.W.; And Others

    The last half of a course in subject indexing for the Educational Resources Information Center (ERIC) system is presented in this volume. The demonstration of indexing techniques, lesson 3, uses a typical document (given in full in an appendix in volume 1) to show how indexing concepts are chosen in practice and converted into terms contained in…

  17. Teaching about Japanese-American Internment. ERIC Digest.

    Science.gov (United States)

    Mukai, Gary

    The Japanese-American internment experience during World War II continues to affect the Japanese-American community. This ERIC Digest provides six suggestions for teaching about the Japanese-American internment, touching on civil rights, immigration, the media, "loyalty," redress and reparations, and primary and secondary sources.…

  18. Television Violence and Children. ERIC/EECE Report.

    Science.gov (United States)

    Cesarone, Bernard

    1998-01-01

    Summarizes 12 recent documents and journal articles from the ERIC database that discuss topics related to television violence and children. Articles cited address the effects of television violence on child behavior and attitudes at school and home, and methods of reducing the impact of television on children. (JPB)

  19. Restructuring the Schools. ERIC Digest Series Number EA 37.

    Science.gov (United States)

    Klauke, Amy

    This ERIC digest focuses on school restructuring as the central issue in the school reform movement by answering five questions: (1) What is "restructuring?" (2) What characteristics of the current educational system are the objects of reform? (3) How can restructuring attend to new skills students will need? (4) How can individual schools…

  20. Recruiting and Selecting Principals. ERIC Digest Series Number EA27.

    Science.gov (United States)

    Klauke, Amy

    This ERIC Digest is an overview of the process of recruiting and selecting principals. Criteria for selecting a principal should include problem analysis, organizational ability, decisiveness, effective communication skills, and stress tolerance, along with personal qualities such as dedication, creativity, and enthusiasm. Innovative steps that…

  1. Preparing School Administrators. ERIC Digest Series Number EA 57.

    Science.gov (United States)

    Klauke, Amy

    Issues involved in improving administrator preparation programs are described in this ERIC Digest. A literature review identifies appropriate skills and methods for administrator training and summarizes recommendations such as application of theory to practice and development of full-time internships, school/university collaboration, mentorships,…

  2. Working with Shy or Withdrawn Students. ERIC Digest.

    Science.gov (United States)

    Brophy, Jere

    This ERIC digest focuses on students who are commonly described as shy (inhibited, lacking in confidence, socially anxious) or withdrawn (unresponsive, uncommunicative, or daydreaming). Symptoms of shyness or withdrawal may appear as part of the student's overall personality or as a situation-specific response to a particular stress factor.…

  3. Television Violence and Children. ERIC/EECE Report.

    Science.gov (United States)

    Cesarone, Bernard

    1998-01-01

    Summarizes 12 recent documents and journal articles from the ERIC database that discuss topics related to television violence and children. Articles cited address the effects of television violence on child behavior and attitudes at school and home, and methods of reducing the impact of television on children. (JPB)

  4. Educational Resources Information Center (ERIC) File Partition Study: Final Report.

    Science.gov (United States)

    Hull, Cynthia C.; Wanger, Judith

    A study to provide the National Center for Educational Communication (NCE) with information that could be useful in making the ERIC data base more relevant to the needs of educators and more efficiently usable by them is discussed. Specific purposes of this project were to use an empirical field-survey study as an armature around which to: (1)…

  5. The Great Depression: An ERIC/ChESS Sample.

    Science.gov (United States)

    Kulczak, Carrie

    2001-01-01

    Provides citations with abstracts from the ERIC database focusing on the Great Depression. Includes both background information and teaching materials on such topics as an overview of the New Deal, the arts and the Great Depression, and information on the Civilian Conservation Corps. Offers directions for accessing the materials. (CMK)

  6. Kunst elada piiri peal / Eric Kluitenberg ; interv. Heie Treier

    Index Scriptorium Estoniae

    Kluitenberg, Eric

    1999-01-01

    Võrguteoreetik Eric Kluitenberg Amsterdamist valgustab 'Interstanding 3' 'Ülalpool piire' tagamaad ja seost Eestiga. Üritus toob Eestisse rahvusvahelisi tippteoreetikuid, kes analüüsivad elu piiri peal, ja kunstnikke, kes seda seisundit oma loomingus kajastavad. Osalema kutsutuid. Piiripealsuset seoses poliitika, interneti, meediaga, äärealadest.

  7. Including Students with Disabilities. ERIC Mini-Bib EB14.

    Science.gov (United States)

    Sorenson, Barbara R., Comp.

    This annotated bibliography lists 19 publications to help educators more effectively include students with disabilities in general education classrooms. The publications, including books, journal articles, videotapes, and reports, are dated from 1993 through 1999, and most are available through the ERIC (Educational Resources Information Center)…

  8. Trends in Library and Information Science: 1989. ERIC Digest.

    Science.gov (United States)

    Eisenberg, Michael B.

    Based on a content analysis of professional journals, conference proceedings, ERIC documents, annuals, and dissertations in library and information science, the following current trends in the field are discussed: (1) there are important emerging roles and responsibilities for information professionals; (2) the status and image of librarians…

  9. Rep rated long life capacitor development: Phase 1 and 2

    Science.gov (United States)

    Galperin, I.; White, W.; Haskell, K.; Ennis, J.

    1984-09-01

    Polypropylene and polyolefin resins were characterized and processed into capacitor films. New capacitor films were developed--a post oriented processed, two layer polypropylene film and upgraded polyolefin film. Criteria for judging film quality were developed. Field life data were verified with paper/polypropylene capacitors on the Maxwell rep rate facility. A11 polypropylene film capacitors were developed with lifetimes in excess of 10 to the 7th power discharges at energy densities for the finished capacitor of 18.3 J/lb and projected to more than 20 J/lb.

  10. República: gobierno gerencial vs política

    Directory of Open Access Journals (Sweden)

    Miguel Ángel Sánchez Ramos

    2008-01-01

    Full Text Available El presente artículo tiene como propósito revisar el modelo en el que se basó el Partido Acción Nacional (PAN, al gobernar en la presidencia de la República, con Vicente Fox como presidente, demostrando que descuidó la política al preferir los elementos organizacionales por encima de ésta y no reconociéndola como un mecanismo necesario para la disminución de las contradicciones en una sociedad con alta diferenciación social.

  11. A Economia da República Velha,1889-1930

    OpenAIRE

    Gustavo H. B. Franco; Luiz Aranha Correa do Lago

    2011-01-01

    1. Preâmbulo: transição, legado e desafios 2. Do Encilhamento ao Funding Loan 3. O Convênio de Taubaté: a defesa do café e do câmbio 4. O desempenho da economia de 1889 a 1913 em perspectiva 5. A primeira Guerra Mundial, e os “choques adversos” 6. A reconstrução da normalidade nos anos 1920 7. A economia brasileira ao final da República Velha 8. Bibliografia

  12. Stress Monitoring of Mulberry Plants by Finding Rep Using Hyperspectral Data

    Science.gov (United States)

    Bhosle, K.; Musande, V.

    2017-05-01

    Stressed on crop can be monitored using different indices. Red Edge position is good estimator for stress monitoring. The red edge position (REP) is strongly correlated with foliar chlorophyll content. Strong chlorophyll absorption causes the abrupt change in the 680-800 nm region of reflectance spectra of green vegetation. The red edge consist the point of maximum slope between red and near-infrared wavelengths. REP can be used to recognize green zone of the observation area. The REP is present in spectra for vegetation recorded by remote sensing methods. REP is clearer and significant in hyper spectral data as hyper spectral consist of more and continuous bands data. In this paper experiments were carried out for mulberry crop using USGS EO-1 Hyperion data. Atmospheric corrected data is used for classification. Classification is carried out on small cluster of 14 field samples. Ground truth is verified and classified by comparing with Hyperion data. REP is different for different stressed condition of crops and shows healthy and diseased crop condition. Nutritional stresses, diseases, drought of plants are detected using REP. Stressed and healthy field of mulberry are estimated by calculating REP using maximum first derivative, linear interpolation, linear extrapolation method. Finally REP is compared using above methods. It is noticeable difference of REP for healthy and stressed crop. The research indicates that overall accuracy using maximum first derivative was 92.85 % and it was more compared to other methods. Linear extrapolation gives less accuracy compared to linear interpolation method.

  13. Mis saab sinu andekast lapsest? / Tõnis Lukas, Mailis Reps, Peeter Kreitzberg...[jt.] ; intervjueerinud Urve Eslas

    Index Scriptorium Estoniae

    2011-01-01

    Haridusteemalistele küsimustele vastavad haidusminister Tõnis Lukas (IRL), reformierakondlane Paul-Eerik Rummo, sotsiaaldemokraat Peeter Kreitzberg, keskerakondlane Mailis Reps ja Raivo Juurak roheliste nimekirjast

  14. Distance Education at the Elementary and Secondary Level. A Select ERIC Bibliography. ERIC/IR Mini-Bib.

    Science.gov (United States)

    Preston, Nancy R., Comp.

    This annotated bibliography lists 10 articles and documents selected through a search of the Educational Resources Information Center (ERIC) database. They are: (1) "The Use of Computers in the Instructional Process in Australian Distance Education" (Geoff Arger and Debbie Clayton); (2) "Distance Education Technologies: All That Glitters Is Not…

  15. Application evaluation of ERIC-PCR on genotyping of Acinetobacter baumannii%ERIC-PCR技术在鲍曼不动杆菌基因分型中的应用评估

    Institute of Scientific and Technical Information of China (English)

    李永丽; 应春妹; 陈艺升

    2013-01-01

    目的 了解仁济医院鲍曼不动杆菌的基因同源性,验证肠杆菌科基因间重复序列(ERIC)-聚合酶链反应(PCR)在鲍曼不动杆菌基因分型中的实用性.方法 采用琼脂稀释法检测临床分离的81株鲍曼不动杆菌对临床常用抗菌药物的最低抑菌浓度(MIC);采用ERIC-PCR对81株鲍曼不动杆菌进行基因分型;使用脉冲场凝胶电泳(PFGE)技术对其中的43株耐亚胺培南鲍曼不动杆菌进一步分型,以PFGE分型为参考标准,验证ERIC-PCR在基因分型中的实用性.结果 81株鲍曼不动杆菌对临床常用的13种抗菌药物普遍耐药,只对米诺环素耐药率最低,为30.9%,其次是亚胺培南,为53.1%,对其他抗菌药物的耐药率均>60%.81株鲍曼不动杆菌ERIC-PCR分型主要为A、B、C、D、E、F6型,其中A、B、C型为主要的流行株.43株耐亚胺培南鲍曼不动杆菌分型为A型41株(A1型29株,A2型12株)、B型1株、C型1株.PFGE分型将其分为5型,A型34株(A1型31株,A2型3株),B型6株,C、D、E分型各1株.结论 仁济医院鲍曼不动杆菌存在克隆播散,且主要为耐亚胺培南鲍曼不动杆菌.ERIC-PCR分辨力较强,结果可靠,与PFGE结果具有一定的相关性,是一种简便、快捷的基因分型技术.%Objective To investigate the gene homology of Acinetobacter baumannii clinical isolates in Renji Hospital and to demonstrate the role of enterobacterial repetitive intergenic consensus (ERIC)-polymerase chain reaction (PCR) on genotyping of Acinetobacter baumannii.Methods The minimum inhibitory concentrations (MIC) of common clinical antibiotics against 81 isolates of Acinetobacter baumannii were determined by agar dilution method.ERIC-PCR was used to type 81 isolates of Acinetobacter baumannii.Pulsed-field gel electrophoresis (PFGE) as reference standard was used to further type 43 imipenem-resistant Acinetobacter baumannii.The practicability of ERIC-PCR was verified.Results The 81 isolates of

  16. A short pulse, high rep-rate microdischarge VUV source

    Science.gov (United States)

    Stephens, Jacob; Fierro, Andrew; Dickens, James; Neuber, Andreas; CenterPulsed Power; Power Electronics Team

    2013-09-01

    A MOSFET based high voltage pulser is utilized to excite a microdischarge (MD), or microdischarge array (MDA) with pulsed voltages of up to 1 kV, with risetime and FWHM on the order of 10 ns and 30 ns, respectively. Additionally, the pulser is capable of pulsing at rep-rates in excess of 35 MHz. However, for these experiments the rep-rate was set on the order of 1 MHz, so as to limit excess energy deposition into the background gas and optimize the energy efficiency of VUV generation. Using VUV capable spectral diagnostics, the VUV emission of the MDs for various pressures (50-800 + Torr) and gases, focused on argon, argon-hydrogen mixtures, and neon-hydrogen mixtures (all of which provide strong emission at λ VUV emission is characterized and compared to results from transient fluid modeling of the MDA. For instance, the MDA turn-on time is recorded to be about 15 ns, which matches the full plasma development time in the model, and the MDA self- capacitance largely determines the turn-off behavior. This research was supported by an AFOSR grant on the Physics of Distributed Plasma Discharges and fellowships from the National Physical Sciences Consortium, supported by Sandia National Laboratories.

  17. Comparison of pulsed-field gel electrophoresis & repetitive sequence-based PCR methods for molecular epidemiological studies of Escherichia coli clinical isolates

    Directory of Open Access Journals (Sweden)

    Il Kwon Bae

    2014-01-01

    Full Text Available Background & objectives: PFGE, rep-PCR, and MLST are widely used to identify related bacterial isolates and determine epidemiologic associations during outbreaks. This study was performed to compare the ability of repetitive sequence-based PCR (rep-PCR and pulsed-field gel electrophoresis (PFGE to determine the genetic relationships among Escherichia coli isolates assigned to various sequence types (STs by two multilocus sequence typing (MLST schemes. Methods: A total of 41 extended-spectrum β-lactamase- (ESBL- and/or AmpC β-lactamase-producing E. coli clinical isolates were included in this study. MLST experiments were performed following the Achtman′s MLST scheme and the Whittam′s MLST scheme, respectively. Rep-PCR experiments were performed using the DiversiLab system. PFGE experiments were also performed. Results: A comparison of the two MLST methods demonstrated that these two schemes yielded compatible results. PFGE correctly segregated E. coli isolates belonging to different STs as different types, but did not group E. coli isolates belonging to the same ST in the same group. Rep-PCR accurately grouped E. coli isolates belonging to the same ST together, but this method demonstrated limited ability to discriminate between E. coli isolates belonging to different STs. Interpretation & conclusions: These results suggest that PFGE would be more effective when investigating outbreaks in a limited space, such as a specialty hospital or an intensive care unit, whereas rep-PCR should be used for nationwide or worldwide epidemiology studies.

  18. Repetition in English Political Public Speaking

    Institute of Scientific and Technical Information of China (English)

    李红梅

    2010-01-01

    Repetition is frequently used in English political public speaking to make it easy to be remembered and powerful to move the feelings of the public. This paper is intended to analyze the functions of repetition and different levels of repetition to highlight the significance of repetition in English political public speaking and the ability of using it in practice.

  19. The development of a repetition-load scheme for the eccentric-only bench press exercise.

    Science.gov (United States)

    Moir, Gavin L; Erny, Kyle F; Davis, Shala E; Guers, John J; Witmer, Chad A

    2013-01-01

    The purpose of the present study was to develop a repetition-load scheme for the eccentric-only bench press exercise. Nine resistance trained men (age: 21.6 ± 1.0 years; 1-repetition maximum [RM] bench press: 137.7 ± 30.4 kg) attended four testing sessions during a four week period. During the first session each subject's 1-RM bench press load utilizing the stretch-shortening cycle was determined. During the remaining sessions they performed eccentric-only repetitions to failure using supra-maximal loads equivalent to 110%, 120% and 130% of their 1-RM value with a constant cadence (30 reps·min(-1)). Force plates and a three dimensional motion analysis system were used during these final three sessions in order to evaluate kinematic and kinetic variables. More repetitions were completed during the 110% 1-RM condition compared to the 130% 1-RM condition (p=0.01). Mean total work (p=0.046) as well as vertical force (p=0.049), vertical work (p=0.017), and vertical power output (p=0.05) were significantly greater during the 130% 1-RM condition compared to the 110% 1-RM condition. A linear function was fitted to the number of repetitions completed under each load condition that allowed the determination of the maximum number of repetitions that could be completed under other supra-maximal loads. This linear function predicted an eccentric-only 1-RM in the bench press with a load equivalent to 164.8% 1-RM, producing a load of 227.0 ± 50.0 kg. The repetition-load scheme presented here should provide a starting point for researchers to investigate the kinematic, kinetic and metabolic responses to eccentric-only bench press workouts.

  20. Eric Hobsbawm, sociólogo do milenarismo campesino Eric Hobsbawm, sociologist of peasant millenarianism

    Directory of Open Access Journals (Sweden)

    Michael Löwy

    2010-01-01

    Full Text Available Graças à problemática do milenarismo, a historiografia de Eric Hobsbawm integra toda a riqueza da subjetividade sociocultural, a profundidade das crenças, sentimentos e emoções em sua análise dos acontecimentos históricos, que não são mais, nessa perspectiva, percebidos simplesmente como produtos do jogo "objetivo" das forças econômicas ou políticas. Ainda distinguindo cuidadosamente os milenarismos primitivos dos revolucionários modernos, não deixa de mostrar sua afinidade eletiva. Isso não quer dizer que todos os movimentos revolucionários sejam milenaristas em sentido estrito ou, pior todavia, que respondam a um quiliasmo de tipo primitivo. Isso não impede afirmar que a afinidade entre os dois seja um fato fundamental na história das revoltas camponesas contra a modernização capitalista. Trata-se de uma das hipóteses de investigação mais interessantes esquematizadas em seus trabalhos desta época. Hobs-bawm ilustra seus propósitos com dois estudos de caso apaixonantes: o anarquismo rural na Andaluzia e as ligas camponesas da Sicília, os dois surgidos em fins do século XIX com prolongamentos no XX.Thanks to the problematic of millenarianism, Eric Hobsbawm's historiography incorporates all the richness of socio-cultural subjectivity - the depth of beliefs, feelings and emotions - into his analysis of historical events, which, from this viewpoint, are no longer perceived simply as products of the "objective" interplay of economic or political forces. Although he makes a careful distinction between primitive millenarianisms and modern revolutionary movements, Hobsbawm nevertheless shows their elective affinity between them. This does not mean that all revolutionary movements are millenarian in the strict sense or - which is even worse - that they are connected to a primitive type of chiliasm. All the same, the affinity between them is a basic fact in the history of peasant revolts against capitalist modernization. This

  1. Varianish: Jamming with Pattern Repetition

    Directory of Open Access Journals (Sweden)

    Jort Band

    2014-10-01

    Full Text Available In music, patterns and pattern repetition are often regarded as a machine-like task, indeed often delegated to drum Machines and sequencers. Nevertheless, human players add subtle differences and variations to repeated patterns that are musically interesting and often unique. Especially when looking at minimal music, pattern repetitions create hypnotic effects and the human mind blends out the actual pattern to focus on variation and tiny differences over time. Varianish is a musical instrument that aims at turning this phenomenon into a new musical experience for musician and audience: Musical pattern repetitions are found in live music and Varianish generates additional (musical output accordingly that adds substantially to the overall musical expression. Apart from the theory behind the pattern finding and matching and the conceptual design, a demonstrator implementation of Varianish is presented and evaluated.

  2. Structure and function of REP34 implicates carboxypeptidase activity in Francisella tularensis host cell invasion.

    Science.gov (United States)

    Feld, Geoffrey K; El-Etr, Sahar; Corzett, Michele H; Hunter, Mark S; Belhocine, Kamila; Monack, Denise M; Frank, Matthias; Segelke, Brent W; Rasley, Amy

    2014-10-31

    Francisella tularensis is the etiological agent of tularemia, or rabbit fever. Although F. tularensis is a recognized biothreat agent with broad and expanding geographical range, its mechanism of infection and environmental persistence remain poorly understood. Previously, we identified seven F. tularensis proteins that induce a rapid encystment phenotype (REP) in the free-living amoeba, Acanthamoeba castellanii. Encystment is essential to the pathogen's long term intracellular survival in the amoeba. Here, we characterize the cellular and molecular function of REP34, a REP protein with a mass of 34 kDa. A REP34 knock-out strain of F. tularensis has a reduced ability to both induce encystment in A. castellanii and invade human macrophages. We determined the crystal structure of REP34 to 2.05-Å resolution and demonstrate robust carboxypeptidase B-like activity for the enzyme. REP34 is a zinc-containing monomeric protein with close structural homology to the metallocarboxypeptidase family of peptidases. REP34 possesses a novel topology and substrate binding pocket that deviates from the canonical funnelin structure of carboxypeptidases, putatively resulting in a catalytic role for a conserved tyrosine and distinct S1' recognition site. Taken together, these results identify REP34 as an active carboxypeptidase, implicate the enzyme as a potential key F. tularensis effector protein, and may help elucidate a mechanistic understanding of F. tularensis infection of phagocytic cells.

  3. Identification of CTX-M15-, SHV-28-producing Klebsiella pneumoniae ST15 as an epidemic clone in the Copenhagen area using a semi-automated Rep-PCR typing assay

    DEFF Research Database (Denmark)

    Nielsen, J B; Skov, M N; Jørgensen, R L;

    2011-01-01

    Rapid molecular typing methods can be a valuable aid in the investigation of suspected outbreaks. We used a semi-automated repetitive sequence-based polymerase chain reaction (Rep-PCR) typing assay and pulsed field gel electrophoresis (PFGE) to investigate the relationship between local Klebsiella...... pneumoniae (K. pneumoniae) producing extended spectrum β-lactamases (ESBLs) and their relation to recognized Danish outbreak strains. PFGE and Rep-PCR produced similar clustering among isolates. Individual isolates from each cluster were further characterized by PCR amplification and sequencing of bla (TEM......), bla (SHV), and bla (CTX-M), and multilocus sequence typing (MLST). Thirty-five out of 52 ESBL-producing K. pneumoniae isolates were ST15 and bla (CTX-M15), bla (SHV-28), and bla (TEM-1) positive by PCR. Ten out of 52 were ST16 and tested positive for bla (CTX-M15), bla (SHV-1), and bla (TEM-1...

  4. REPETITIVE CLUSTER-TILTED ALGEBRAS

    Institute of Scientific and Technical Information of China (English)

    Zhang Shunhua; Zhang Yuehui

    2012-01-01

    Let H be a finite-dimensional hereditary algebra over an algebraically closed field k and CFm be the repetitive cluster category of H with m ≥ 1.We investigate the properties of cluster tilting objects in CFm and the structure of repetitive clustertilted algebras.Moreover,we generalize Theorem 4.2 in [12](Buan A,Marsh R,Reiten I.Cluster-tilted algebra,Trans.Amer.Math.Soc.,359(1)(2007),323-332.) to the situation of CFm,and prove that the tilting graph KCFm of CFm is connected.

  5. Seis nuevas bibliotecas del Banco de la República

    Directory of Open Access Journals (Sweden)

    Boletín Cultural y Bibliográfico Banco de la República

    2000-05-01

    Full Text Available En febrero de 2000, el Banco de la República anunció la decisión de suspender el servicio de tesorería en algunas sucursales y reforzar las áreas culturales, convirtiendo estas últimas en bibliotecas. A partir del 1 de septiembre de 2000 se inicia la adecuación de espacios en las sucursales de Santa Marta, Sincelejo, Valledupar, Florencia y Popayán para albergar en cada una de ellas una biblioteca pública con servicios de lectura para niños y adultos; y en las bibliotecas de Girardot, Honda, Ipiales y Tunja, sucursales que contaban con servicios bibliotecarios a cargo del Banco, se amplían los espacios destinados al servicio de consulta

  6. Madison e Dahl: da república a poliarquia

    OpenAIRE

    José Alexandre Silva Junior; Dalson Britto Figueiredo Filho; Ranulfo Paranhos; Gabriel Augusto Miranda Setti; Willber Nascimento

    2014-01-01

    http://dx.doi.org/10.5007/1806-5023.2014v11n2p42 Qual é a relação entre as teorias política clássica e contemporânea? O principal objetivo desse trabalho é responder a referida questão. Em termos metodológicos, examinam-se alguns conceitos analisados por James Madison e Robert Dahl em perspectiva comparada. Em particular, o foco repousa sobre as noções madisonianas de facção, separação de poderes e república e sobre o conceito de poliarquia proposto por Dahl. Dessa maneira, descrevemos a ...

  7. Automated Production of High Rep Rate Foam Targets

    Science.gov (United States)

    Hall, F.; Spindloe, C.; Haddock, D.; Tolley, M.; Nazarov, W.

    2016-04-01

    Manufacturing low density targets in the numbers needed for high rep rate experiments is highly challenging. This report summarises advances from manual production to semiautomated and the improvements that follow both in terms of production time and target uniformity. The production process is described and shown to be improved by the integration of an xyz robot with dispensing capabilities. Results are obtained from manual and semiautomated production runs and compared. The variance in the foam thickness is reduced significantly which should decrease experimental variation due to target parameters and could allow for whole batches to be characterised by the measurement of a few samples. The work applies to both foil backed and free standing foam targets.

  8. Integral objects and Deligne's category Rep(S_t)

    CERN Document Server

    Del Padrone, Alessio

    2010-01-01

    We give negative answers to certain questions on abelian semisimple tensor categories raised by Bruno Kahn and Charles A. Weibel in connection with the preprint of Kahn "On the multiplicities of a motive" (arXiv:math/0610446), now published as Parts I and IV of "Zeta functions and motives", Pure Appl. Math. Q. 5 (2009), no. 1, part 2, 507-570. For the most interesting examples we used Deligne's category Rep(S_t,F) of representations of the "symmetric group S_t with t not an integer" with F any algebraically closed field of characteristic zero. This is an interesting family of tensor categories, "new" in some sense, interpolating the representations of the symmetric groups. Among other things we give two proofs that this category is not Schur-finite, showing hence explicitly that it is not tensor equivalent to a category of superepresentations of a supergroup.

  9. 3D Image Synthesis for B—Reps Objects

    Institute of Scientific and Technical Information of China (English)

    黄正东; 彭群生; 等

    1991-01-01

    This paper presents a new algorithm for generating 3D images of B-reps objects with trimmed surface boundaries.The 3D image is a discrete voxel-map representation within a Cubic Frame Buffer (CFB).The definition of 3D images for curve,surface and solid object are introduced which imply the connectivity and fidelity requirements.Adaptive Forward Differencing matrix (AFD-matrix) for 1D-3D manifolds in 3D space is developed.By setting rules to update the AFD-matrix,the forward difference direction and stepwise can be adjusted.Finally,an efficient algorithm is presented based on the AFD-matrix concept for converting the object in 3D space to 3D image in 3D discrete space.

  10. Developmental biologist Eric H. Davidson, 1937–2015

    OpenAIRE

    Hood, Leroy; Rothenberg, Ellen V.

    2015-01-01

    Eric H. Davidson, a world leader in developmental biology, demonstrated that most of development is, indeed, regulated by the genome. He was a pioneer researcher and theorist of the gene regulatory networks that execute the most complex biological processes, such as the cascade of molecular mechanisms that transform a single-celled egg into a complex creature. He insisted that the seemingly infinite details of classical developmental biology had to be explained in terms of the function of DNA...

  11. Fans of Columbine shooters Eric Harris and Dylan Klebold

    OpenAIRE

    Andrew Ryan Rico

    2015-01-01

    On April 20, 1999, Eric Harris and Dylan Klebold murdered 12 students and one teacher at Columbine High School in Littleton, Colorado, in what was then the deadliest school shooting in American history. Despite causing a national panic and serving as a flash point for larger narratives on bullying, gun control, and media violence, both boys have gained active online fans. These fandoms dedicated to the Columbine shooters are widely referred to as dark examples of Internet communities, while t...

  12. Repetitive elements in parasitic protozoa

    Directory of Open Access Journals (Sweden)

    Clayton Christine

    2010-05-01

    Full Text Available Abstract A recent paper published in BMC Genomics suggests that retrotransposition may be active in the human gut parasite Entamoeba histolytica. This adds to our knowledge of the various types of repetitive elements in parasitic protists and the potential influence of such elements on pathogenicity. See research article http://www.biomedcentral.com/1471-2164/11/321

  13. 75 FR 27840 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-05-18

    ...: 2010-11842] NUCLEAR REGULATORY COMMISSION [NRC-2010-0080] NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3...: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for General... the existing guidance contained in Supplement 3 to NUREG- 0654/FEMA-REP-1, Rev. 1, ``Criteria...

  14. 75 FR 10524 - NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-03-08

    ... COMMISSION NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for... document entitled: ``NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3, Guidance for Protective Action...-4737, or by e-mail to pdr.resource@nrc.gov . NUREG-0654/FEMA-REP-1, Rev. 1, Supplement 3,...

  15. Repetition suppression and repetition priming are processing outcomes.

    Science.gov (United States)

    Wig, Gagan S

    2012-01-01

    Abstract There is considerable evidence that repetition suppression (RS) is a cortical signature of previous exposure to the environment. In many instances RS in specific brain regions is accompanied by improvements in specific behavioral measures; both observations are outcomes of repeated processing. In understanding the mechanism by which brain changes give rise to behavioral changes, it is important to consider what aspect of the environment a given brain area or set of areas processes, and how this might be expressed behaviorally.

  16. STRESS MONITORING OF MULBERRY PLANTS BY FINDING REP USING HYPERSPECTRAL DATA

    Directory of Open Access Journals (Sweden)

    K. Bhosle

    2017-05-01

    Full Text Available Stressed on crop can be monitored using different indices. Red Edge position is good estimator for stress monitoring. The red edge position (REP is strongly correlated with foliar chlorophyll content. Strong chlorophyll absorption causes the abrupt change in the 680–800 nm region of reflectance spectra of green vegetation. The red edge consist the point of maximum slope between red and near-infrared wavelengths. REP can be used to recognize green zone of the observation area. The REP is present in spectra for vegetation recorded by remote sensing methods. REP is clearer and significant in hyper spectral data as hyper spectral consist of more and continuous bands data. In this paper experiments were carried out for mulberry crop using USGS EO-1 Hyperion data. Atmospheric corrected data is used for classification. Classification is carried out on small cluster of 14 field samples. Ground truth is verified and classified by comparing with Hyperion data. REP is different for different stressed condition of crops and shows healthy and diseased crop condition. Nutritional stresses, diseases, drought of plants are detected using REP. Stressed and healthy field of mulberry are estimated by calculating REP using maximum first derivative, linear interpolation, linear extrapolation method. Finally REP is compared using above methods. It is noticeable difference of REP for healthy and stressed crop. The research indicates that overall accuracy using maximum first derivative was 92.85 % and it was more compared to other methods. Linear extrapolation gives less accuracy compared to linear interpolation method.

  17. Cohesive Function of Lexical Repetition in Text

    Institute of Scientific and Technical Information of China (English)

    张莉; 卢沛沛

    2013-01-01

    Lexical repetition is the most direct form of lexical cohesion,which is the central device for making texts hang together. Although repetition is the most direct way to emphasize,it performs the cohesive effect more apparently.

  18. Two families of rep-like genes that probably originated by interspecies recombination are represented in viral, plasmid, bacterial, and parasitic protozoan genomes.

    Science.gov (United States)

    Gibbs, Mark J; Smeianov, Vladimir V; Steele, James L; Upcroft, Peter; Efimov, Boris A

    2006-06-01

    Two families of genes related to, and including, rolling circle replication initiator protein (Rep) genes were defined by sequence similarity and by evidence of intergene family recombination. The Rep genes of circoviruses were the best characterized members of the "RecRep1 family." Other members of the RecRep1 family were Rep-like genes found in the genomes of the Canarypox virus, Entamoeba histolytica, and Giardia duodenalis and in a plasmid, p4M, from the Gram-positive bacterium, Bifidobacterium pseudocatenulatum. The "RecRep2 family" comprised some previously identified Rep-like genes from plasmids of phytoplasmas and similar Rep-like genes from the genomes of Lactobacillus acidophilus, Lactococcus lactis, and Phytoplasma asteris. Both RecRep1 and RecRep2 proteins have a nucleotide-binding domain significantly similar to the helicases (2C proteins) of picorna-like viruses. On the N-terminal side of the nucleotide binding domain, RecRep1 proteins have a domain significantly similar to one found in nanovirus Reps, whereas RecRep2 proteins have a domain significantly similar to one in the Reps of pLS1 plasmids. We speculate that RecRep genes have been transferred from viruses or plasmids to parasitic protozoan and bacterial genomes and that Rep proteins were themselves involved in the original recombination events that generated the ancestral RecRep genes.

  19. DNA structure modulates the oligomerization properties of the AAV initiator protein Rep68.

    Directory of Open Access Journals (Sweden)

    Jorge Mansilla-Soto

    2009-07-01

    Full Text Available Rep68 is a multifunctional protein of the adeno-associated virus (AAV, a parvovirus that is mostly known for its promise as a gene therapy vector. In addition to its role as initiator in viral DNA replication, Rep68 is essential for site-specific integration of the AAV genome into human chromosome 19. Rep68 is a member of the superfamily 3 (SF3 helicases, along with the well-studied initiator proteins simian virus 40 large T antigen (SV40-LTag and bovine papillomavirus (BPV E1. Structurally, SF3 helicases share two domains, a DNA origin interaction domain (OID and an AAA(+ motor domain. The AAA(+ motor domain is also a structural feature of cellular initiators and it functions as a platform for initiator oligomerization. Here, we studied Rep68 oligomerization in vitro in the presence of different DNA substrates using a variety of biophysical techniques and cryo-EM. We found that a dsDNA region of the AAV origin promotes the formation of a complex containing five Rep68 subunits. Interestingly, non-specific ssDNA promotes the formation of a double-ring Rep68, a known structure formed by the LTag and E1 initiator proteins. The Rep68 ring symmetry is 8-fold, thus differing from the hexameric rings formed by the other SF3 helicases. However, similiar to LTag and E1, Rep68 rings are oriented head-to-head, suggesting that DNA unwinding by the complex proceeds bidirectionally. This novel Rep68 quaternary structure requires both the DNA binding and AAA(+ domains, indicating cooperativity between these regions during oligomerization in vitro. Our study clearly demonstrates that Rep68 can oligomerize through two distinct oligomerization pathways, which depend on both the DNA structure and cooperativity of Rep68 domains. These findings provide insight into the dynamics and oligomeric adaptability of Rep68 and serve as a step towards understanding the role of this multifunctional protein during AAV DNA replication and site-specific integration.

  20. At Home with ERIC: Online Searching from Your Home Computer. "Knowledge Index" Edition.

    Science.gov (United States)

    Gonzalez-Stupp, Emma; Wildemuth, Barbara M.

    This manual provides information to aid home computer owners in performing literature searches of ERIC and other education and social science databases, such as the DIALOG Information Services "Knowledge Index" System. Assuming some familiarity with printed ERIC indexes or online searches, this manual is divided into five chapters. It is…

  1. At Home with ERIC: Online Searching from Your Home Computer. "After Dark" Edition.

    Science.gov (United States)

    Gonzalez-Stupp, Emma; Wildemuth, Barbara M.

    This manual provides information to aid home computer owners in performing literature searches of ERIC and other education and social science databases using the BRS Information Technologies "After Dark" system. Assuming some familiarity with printed ERIC indexes or online searches, this manual is divided into five chapters. It is…

  2. ERIC--The First 15 Years. A History of the Educational Resources Information Center.

    Science.gov (United States)

    Trester, Delmer J.

    This account of the Educational Resources Information Center (ERIC) provides information on its background and origin, and traces the development of the system from initial planning in 1962 through mid-1979. Although this is essentially an overview of the growth of the system, some of the more complex aspects of the ERIC story are included in the…

  3. Reliability and validity of the five-repetition sit-to-stand test for children with cerebral palsy.

    Science.gov (United States)

    Wang, Tze-Hsuan; Liao, Hua-Fang; Peng, Yi-Chun

    2012-07-01

    OBJECTIVE To investigate the psychometric properties of the five-repetition sit-to-stand test, a functional strength test, in children with spastic diplegia. Methodology study. Hospital, laboratory or home. In total, 108 children with spastic diplegia and 62 with typical development aged from five to 12 years were tested. For test-retest reliability, 22 children with spastic diplegia were tested twice within one week. Not applicable. The five-repetition sit-to-stand test measures time needed to complete five consecutive sit-to-stand cycles as quickly as possible. The higher the rate of five-repetition sit-to-stand (repetitions per second), the more strength a person has. The intraclass correlation coefficients of intra-session reliability and test-retest reliability were 0.95 and 0.99 respectively. The minimal detectable difference was 0.06 rep/sec. The convergent validity of the five-repetition sit-to-stand test was supported by significant correlation with one-repetition maximum of the loaded sit-to-stand test, isometric muscle strength, scores of Gross Motor Function Measure, and gait function (r or rho = 0.40-0.78). For known group validity, children with typical development and children classified as Gross Motor Function Classification System level I performed higher rates of five-repetition sit-to-stand than children classified as level II, and children classified as level II performed higher rates than level III. The five-repetition sit-to-stand test was a reliable and valid test to measure functional muscle strength in children with spastic diplegia in clinics.

  4. Genotyping and genetic diversity of Arcobacter butzleri and Arcobacter cryaerophilus isolated from different sources by using ERIC-PCR from India.

    Science.gov (United States)

    Ramees, Thadiyam Puram; Rathore, Ramswaroop Singh; Bagalkot, Prashanth Suresh; Sailo, Blessa; Mohan, Hosakote Venkatappa; Kumar, Ashok; Dhama, Kuldeep; Singh, Raj Kumar

    2014-01-01

    Arcobacters are important zoonotic pathogens and are transmitted through food and water. They are implicated in causing enteritis in animals and humans. Among the Arcobacter species, a wide genetic diversity has been documented, which reflects continuous evolving nature of these pathogens. To genotype and to know the genetic diversity of Arcobacter spp. (Arcobacter butzleri and Arcobacter cryaerophilus) isolated from different sources in India. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) was performed using genomic DNA of 49 Arcobacter isolates (27 A. butzleri and 22 A. cryaerophilus), recovered from a total of 506 samples of chicken meat, poultry skin, dairy cow milk and human stool as template and employing published primers. ERIC sequence was found to be present in all the 27 A. butzleri isolates which were grouped into 18 subtypes, while it was present in 20 out of 22 A. cryaerophilus isolates which were grouped into 14 subtypes. Less variation was observed within sequences of both the Arcobacter species as revealed in dendrogram analysis. The genotyping of A. butzleri isolates showed the presence of 2-8 distinct bands (∼150 to ∼1600 bp), while A. cryaerophilus showed 1-10 distinct bands (∼120 to ∼2900 bp). This study is the first report regarding genetic diversity of Indian Arcobacter isolates using ERIC-PCR. Close clustering between arcobacters of human and animal origin are indicative of probable zoonotic significance. So for these purposes, further explorative studies are suggested which would also help revealing the possibility of epidemiological relationships of different Arcobacter spp. as well as their public health concerns.

  5. Madison e Dahl: da república a poliarquia

    Directory of Open Access Journals (Sweden)

    José Alexandre Silva Junior

    2014-12-01

    Full Text Available http://dx.doi.org/10.5007/1806-5023.2014v11n2p42 Qual é a relação entre as teorias política clássica e contemporânea? O principal objetivo desse trabalho é responder a referida questão. Em termos metodológicos, examinam-se alguns conceitos analisados por James Madison e Robert Dahl em perspectiva comparada. Em particular, o foco repousa sobre as noções madisonianas de facção, separação de poderes e república e sobre o conceito de poliarquia proposto por Dahl. Dessa maneira, descrevemos a continua influência de certos aspectos próprios da teoria política clássica no modo como a teoria contemporânea lida com o tema. Concluímos que a contribuição dos autores clássicos permite que a teoria política contemporânea seja capaz de tentar oferecer explicações mais robustas aos “novos” problemas enfrentados pela disciplina.

  6. Paradoxo e natureza no livro V da República

    Directory of Open Access Journals (Sweden)

    Marcelo P. Marques

    2010-12-01

    Full Text Available O artigo analisa a primeira onda do livro V da República de Platão, levando em conta a oposição entre opinião (dóxa e ciência (epistéme, só formulada ao final da terceira onda. Sócrates problematiza a opinião corrente que aceita a diferença de natureza entre homens e mulheres para avançar a ideia de uma natureza humana comum. É essa tese paradoxal que justifica que possam ter uma mesma educação e que, assim, possam desempenhar as mesmas funções, vindo a ser guardiões e governantes da cidade.The paper analyses the first wave in Plato´s Republic V, taking into account the opposition between opinion (doxa and science (episteme, formulated at the end of the third wave. Socrates questions the current opinion which accepts the difference in nature between men and women in order to propose the idea of a common human nature. It is this paradoxical thesis that justifies the proposal that all should have the same education, thus allowing them to fulfill the same functions and, eventually, to become guardians and rulers in the city.

  7. China - 60 anos de República Popular

    Directory of Open Access Journals (Sweden)

    Wladimir Pomar

    2009-10-01

    Full Text Available Em dezembro de 1949, após mais de 23 anos de guerras, tanto civis, quanto de resistência contra a agressão japonesa, a China assistiu à fundação da República Popular, com a vitória do Exército Popular de Libertação, tendo à frente o Partido Comunista, sobre os exércitos comandados pelo Guomindang.

  8. Euclides da Cunha e a República

    Directory of Open Access Journals (Sweden)

    Roberto Ventura

    1996-04-01

    Full Text Available A revisão da República é central na obra de Euclides da Cunha, presente em seus artigos de jornal e na maior parte de seus livros. Analisou o regime republicano não só em Os sertões (1902, em que tratou da guerra de Canudos, como em Contrastes e confrontos (1907 e em Á margem da história (1909. Foi testemunha e intérprete dos rumos do novo regime, que ajudara a fundar com artigos de propaganda política no jornal A Província de S. Paulo.The revision of the Republic is a central concern in the works of Euclides da Cunha. It appears in his newspaper articles and in most of his books. He analysed the republican regime not only in Os sertões (Rebellion in the backlands, 1902, in which he deals with the Canudos War, but also in Contrastes e confrontos (1907 and in À margem da história (1909. He both witnessed and interpreted the trends of the new regime which he had helped to set up with his political articles in the newspaper A Província de S. Paulo.

  9. Hariduspoliitikas ilmnevad üksmeele märgid / Mailis Reps

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2006-01-01

    Ilmunud ka: Severnoje Poberezhje 7. juuni lk. 2, Nädaline 10. juuni lk. 2. Haridusminister Mailis Reps Res Publica ja Isamaaliidu ühisest haridusprogrammist ning juba teostumas hariduspoliitilistest eesmärkidest

  10. Reps sai Narvas tõrjuva vastuvõtu osaliseks / Tanel Mazur

    Index Scriptorium Estoniae

    Mazur, Tanel, 1971-

    2005-01-01

    Selle aasta koolirahu kuulutati välja Narva linnuses, rongkäigus osalesid Narva koolide õpilaste hulgas ka üle 1300 teistest piirkondadest saabunud õpilase. Tervituskõnega esines haridus- ja teadusminister Mailis Reps

  11. The Filament-specific Rep1-1 Repellent of the Phytopathogen Ustilago maydis Forms Functional Surface-active Amyloid-like Fibrils

    NARCIS (Netherlands)

    Teertstra, Wieke R.; van der Velden, Gisela J.; de Jong, Jan F.; Kruijtzer, John A. W.; Liskamp, Rob M. J.; Kroon-Batenburg, Loes M. J.; Muller, Wally H.; Gebbink, Martijn F. B. G.; Wosten, Han A. B.

    2009-01-01

    Repellents of the maize pathogen Ustilago maydis are involved in formation of hydrophobic aerial hyphae and in cellular attachment. These peptides, called Rep1-1 to Rep1-11, are encoded by the rep1 gene and result from cleavage of the precursor protein Rep1 during passage of the secretion pathway. U

  12. The Filament-specific Rep1-1 Repellent of the Phytopathogen Ustilago maydis Forms Functional Surface-active Amyloid-like Fibrils

    NARCIS (Netherlands)

    Teertstra, Wieke R.; van der Velden, Gisela J.; de Jong, Jan F.; Kruijtzer, John A. W.; Liskamp, Rob M. J.; Kroon-Batenburg, Loes M. J.; Muller, Wally H.; Gebbink, Martijn F. B. G.; Wosten, Han A. B.

    2009-01-01

    Repellents of the maize pathogen Ustilago maydis are involved in formation of hydrophobic aerial hyphae and in cellular attachment. These peptides, called Rep1-1 to Rep1-11, are encoded by the rep1 gene and result from cleavage of the precursor protein Rep1 during passage of the secretion pathway. U

  13. Eric A. Posner and David Weisbach, Climate Change Justice

    OpenAIRE

    Kelleher, J. Paul

    2016-01-01

    Even when this book was first published in 2010, there was a large literature on the nature of climate change justice. (The 2015 paperback release that occasioned this review makes no changes to the 2010 text.) Of course not all of the climate justice literature to that date was penned by philosophers, but a great of it was and naturally philosophers claim to have much to say about climate justice. So it is somewhat surprising how infrequently Eric Poser and David Weisbach engage with the phi...

  14. Transmission identification of Escherichia coli aerosol in chicken houses to their environments using ERIC-PCR

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    In order to study E. coli aerosol spreading from chicken houses to their surrounding air, air samples, including indoor and outdoor air (upwind 10 and 50 m as well as downwind 10, 50, 100, 200 and 400 m away) of 5 chicken houses were collected using six-stage Andersen microbial samplers and Reuter-Centrifugal samplers (RCS). E. coli concentrations (CFU/m3 air) collected from different sampling sites were calculated. E. coli strains from chicken feces samples were also isolated. Furthermore, the enterobacterial repetitive intergenic consensus (ERIC)-PCR method was applied to amplify the isolated E. coli strain DNA samples. Through the genetic similarity analyses of the E. coli obtained from different sampling sites, the spreading of bioaerosol from animal houses to the ambient air was characterized. The results showed that the isolated E. coli concentrations in indoor air (9―63 CFU/m3) in 5 chicken houses were higher than those in upwind and downwind air, but there were no significant differences between the indoor and downwind sites 10 m away from all the 5 houses (P>0.05). The phylogenetic tree indicated that a part of the E. coli (34.1%) isolated from indoor air had 100% similarity with those isolated from feces, and that most of E. coli isolated (54.5%) from downwind at 10, 50, 100 or even 200 m had 100% similarity with those isolated from indoor air or feces too. But those isolated from upwind air had a lower similarity (73%―92%) with corresponding strains isolated from indoor air or feces. Our results suggested that some strains isolated from downwind air and indoor air originated in the chicken feces, but most of isolates obtained from upwind air samples did not come from the chicken feces or indoor air. Effective hygienic measures should be taken in animal farms to prevent or minimize downwind spreading of microorganism aerosol.

  15. Transmission identification of Escherichia coli aerosol in chicken houses to their environments using ERIC-PCR

    Institute of Scientific and Technical Information of China (English)

    DUAN HuiYong; CHAI TongJie; CAI YuMei; ZHONG ZhaoBing; YAO MeiLing; ZHANG XingXiao

    2008-01-01

    In order to study E. coil aerosol spreading from chicken houses to their surrounding air, air samples,Including Indoor and outdoor air (upwind 10 and 50 m as well as downwind 10, 50, 100, 200 and 400 m away) of 5 chicken houses were collected using six-stage Andersen microbial samplers and Reuter-Centrifugal samplers (RCS). E. coli concentrations (CFU/m3 air) collected from different sampling sites were calculated. E. coli strains from chicken feces samples,were also isolated. Furthermore,the enterobacterial repetitive intergenic consensus (ERIC)-PCR method was applied to amplify the isolated E. coil strain DNA samples. Through the genetic similarity analyses of the E. coli obtained from different sampling sites, the spreading of bioaerosol from animal houses to the ambient air was characterized. The results showed that the isolated E. coli concentrations in indoor air (9-63 CFU/m3) in 5chicken houses were higher than those in upwind and downwind air, but there were no significant differences between the indoor and downwind sites 10 m away from all the 5 houses (P>0.05). The phyIogenetic tree indicated that a part of the E. coli (34.1%) isolated from indoor air had 100% similarity with those Isolated from feces, and that most of E. coli isolated (54.5%) from downwind at 10, 50, 100 or even 200 m had 100% similarity with those isolated from indoor air or feces too. But those isolated from upwind air had a lower similarity (73%-92%) with corresponding strains isolated from indoor air or feces. Our results suggested that some strains isolated from downwind air and indoor air originated in the chicken feces, but most of isolates obtained from upwind air samples did not come from the chicken feces or indoor air. Effective hygienic measures should be taken in animal farms to prevent or minimize downwind spreading of microorganism aerosol.

  16. Circuit considerations for repetitive railguns

    Energy Technology Data Exchange (ETDEWEB)

    Honih, E.M.

    1986-01-01

    Railgun electromagnetic launchers have significant military and scientific potential. They provide direct conversion of electrical energy to projectile kinetic energy, and they offer the hope of achieving projectile velocities greatly exceeding the limits of conventional guns. With over 10 km/sec already demonstrated, railguns are attracting attention for tactical and strategic weapons systems and for scientific equation-of-state research. The full utilization of railguns will require significant improvements in every aspect of system design - projectile, barrel, and power source - to achieve operation on a large scale. This paper will review fundamental aspects of railguns, with emphasis on circuit considerations and repetitive operation.

  17. Repetitive operation of an L-band magnetically insulated transmission line oscillator with metal array cathode

    Science.gov (United States)

    Qin, Fen; Wang, Dong; Xu, Sha; Zhang, Yong; Fan, Zhi-kai

    2016-04-01

    We present the repetitive operation research results of an L-band magnetically insulated transmission line oscillator with metal array cathode (MAC-MILO) in this paper. To ensure a more uniform emission of electrons emitted from the cathode, metal plates with different outer radii and thicknesses are periodically arranged in longitudinal direction on the cathode substrate to act as emitters. The higher order mode depressed MILO (HDMILO) structure is applied to ensure stability of the tube. Comparison experiments are carried out between velvet cathode and MAC MILO driven by a 20 GW/40 Ω/40 ns/20 Hz pulse power system. Experimental results reveal that the MAC has much lower outgassing rate, much longer life time, and higher repetitive stability. The MAC-MILO could work stably with a rep-rate up to 20 Hz at a power level of 550 MW when employing a 350 kV/35 kA electric pulse. The TE11 mode radiation pattern in the farfield region reveals the tube works steadily on the dominant mode. More than 2000 shots have been tested in repetitive mode without any obvious degradation of the detected microwave parameters.

  18. Comparison of a commercially available rep-PCR system (Diversilab(R)) with PCR-ribotyping for typing of Clostridium difficile strains.

    OpenAIRE

    Eckert, C.; van Broeck, Johan; Spigaglia, P.; Burghoffer, B; Delmée, Michel; Mastrantonio, P; Barbut, F

    2011-01-01

    This study compared a rep-PCR method (DiversiLab® system) to PCR-ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR-ribotype 027, different rep-types could be distinguished. Rep-PCR showed a higher discriminatory power than PCR-ribotyping. Nevertheless, this method requires technical skill and visual interpretation of rep-PCR fingerprint patterns may be difficult.

  19. ERIC-PCR Fingerprinting of Fecal Escherichia Coli and Microbial Source Tracking in Non-point Pollution of the Shellfish Culture Area of East China Sea%大肠埃希氏菌ERIC-PCR指纹图谱构建及贝类污染微生物源示踪

    Institute of Scientific and Technical Information of China (English)

    何力; 傅玲琳; 冯立芳; 励建荣

    2012-01-01

    Objective: to establish a host-orign fecal E. Coli DNA fingerprint library by rep-PCR method using ERIC primers from livestock and poultry farms in the shellfish culture area of East China Sea. Moreover, the efficacy of microbial source tracking (MST) based on ERIC-PCR for differentiating host sources of E. Coli from shellfish and their growing waters is also evaluated. Methods: fecal samples of poultry and livestock were obtained as cloacal swabs from different farm sites in Xiangshan Bay. E. Coli isolates were purified from the fecal samples by selective cultivation and API 20E tests. The genomic DNA of E. Coli isolates was extracted by bacterial genomic DNA purification kit and fingerprinted by ERIC-PCR. InfoQuestFPTM software was used to analyze the similarities, rate of correct classification(RCC) and the stability of library. Finally, host sources of E. Coli obtained from shellfish and shellfish growing waters were predicted using MST method by Multi-Dimensional Scaling (MDS). Results: cluster analysis of ERIC-PCR DNA fingerprints of 216 E. Coli isolates revealed 37 clusters. Jack-knife analysis revealed high rate of correct classification(RCC) with 91.7%, 76.9%, 100% and 94.4% of swine, chicken, duck and goose E. Coli isolates classified into the correct host source, respectively. In addition, based on the above host-origin library by discriminant analysis, 12 unknown source strains from shellfish and growing waters were successfully discriminanted with RCC value of 78.8%. Conclusion: this work suggests that ERIC-PCR fingerprinting can be a promising genotypic tool applied in the shellfish growing water management on East Chi na Sea for source identification of fecal pollution.%目的:采用rep-PCR的ERIC引物(ERIC-PCR)构建象山港周边粪污染指示因子大肠埃希氏菌(E.coli)的DNA指纹图谱库,并根据所建DNA指纹图谱库对贝类产品污染进行微生物源示踪(Microbial Source Tracking,MST).方法:在选定的贝类养殖区域周

  20. Eric Hobsbawm y su lectura marxista de la historia

    Directory of Open Access Journals (Sweden)

    Pilatowsky, Mauricio

    2014-06-01

    Full Text Available This paper presents a brief survey of the contributions made by the historian Eric Hobsbawm to the field of History and his Marxist stance, both in his academic work and political practice. According to the author, the circumstances in which the researchers live are determinant to their study; hence this analysis includes a review of his biography. This perspective discusses his interpretation of Marx and Marxism, his understanding of History, his militancy in the Communist Party, his Jewish identity, and how he addresses nationalism in relation to the study of capitalism.En este ensayo se presenta un breve estudio de las aportaciones del historiador Eric Hobsbawm en el campo de la historia y de sus posturas marxistas; tanto en su trabajo académico como en su práctica política. El análisis incluye una revisión de su biografía ya que según el autor los investigadores y su manera de investigar responden a las circunstancias que les toca vivir. Desde esta perspectiva se aborda su interpretación de Marx y del marxismo, su forma de entender la historia, su militancia en el Partido Comunista, su identidad judía y el tratamiento del nacionalismo en su relación con el estudio del capitalismo.

  1. Metal-Induced Stabilization and Activation of Plasmid Replication Initiator RepB

    Science.gov (United States)

    Ruiz-Masó, José A.; Bordanaba-Ruiseco, Lorena; Sanz, Marta; Menéndez, Margarita; del Solar, Gloria

    2016-01-01

    Initiation of plasmid rolling circle replication (RCR) is catalyzed by a plasmid-encoded Rep protein that performs a Tyr- and metal-dependent site-specific cleavage of one DNA strand within the double-strand origin (dso) of replication. The crystal structure of RepB, the initiator protein of the streptococcal plasmid pMV158, constitutes the first example of a Rep protein structure from RCR plasmids. It forms a toroidal homohexameric ring where each RepB protomer consists of two domains: the C-terminal domain involved in oligomerization and the N-terminal domain containing the DNA-binding and endonuclease activities. Binding of Mn2+ to the active site is essential for the catalytic activity of RepB. In this work, we have studied the effects of metal binding on the structure and thermostability of full-length hexameric RepB and each of its separate domains by using different biophysical approaches. The analysis of the temperature-induced changes in RepB shows that the first thermal transition, which occurs at a range of temperatures physiologically relevant for the pMV158 pneumococcal host, represents an irreversible conformational change that affects the secondary and tertiary structure of the protein, which becomes prone to self-associate. This transition, which is also shown to result in loss of DNA binding capacity and catalytic activity of RepB, is confined to its N-terminal domain. Mn2+ protects the protein from undergoing this detrimental conformational change and the observed protection correlates well with the high-affinity binding of the cation to the active site, as substituting one of the metal-ligands at this site impairs both the protein affinity for Mn2+and the Mn2+-driven thermostabilization effect. The level of catalytic activity of the protein, especially in the case of full-length RepB, cannot be explained based only on the high-affinity binding of Mn2+ at the active site and suggests the existence of additional, lower-affinity metal binding site

  2. A Dimeric Rep Protein Initiates Replication of a Linear Archaeal Virus Genome: Implications for the Rep Mechanism and Viral Replication ▿ †

    Science.gov (United States)

    Oke, Muse; Kerou, Melina; Liu, Huanting; Peng, Xu; Garrett, Roger A.; Prangishvili, David; Naismith, James H.; White, Malcolm F.

    2011-01-01

    The Rudiviridae are a family of rod-shaped archaeal viruses with covalently closed, linear double-stranded DNA (dsDNA) genomes. Their replication mechanisms remain obscure, although parallels have been drawn to the Poxviridae and other large cytoplasmic eukaryotic viruses. Here we report that a protein encoded in the 34-kbp genome of the rudivirus SIRV1 is a member of the replication initiator (Rep) superfamily of proteins, which initiate rolling-circle replication (RCR) of diverse viruses and plasmids. We show that SIRV Rep nicks the viral hairpin terminus, forming a covalent adduct between an active-site tyrosine and the 5′ end of the DNA, releasing a 3′ DNA end as a primer for DNA synthesis. The enzyme can also catalyze the joining reaction that is necessary to reseal the DNA hairpin and terminate replication. The dimeric structure points to a simple mechanism through which two closely positioned active sites, each with a single tyrosine residue, work in tandem to catalyze DNA nicking and joining. We propose a novel mechanism for rudivirus DNA replication, incorporating the first known example of a Rep protein that is not linked to RCR. The implications for Rep protein function and viral replication are discussed. PMID:21068244

  3. Electra: durable repetitively pulsed angularly multiplexed KrF laser system

    Science.gov (United States)

    Wolford, Matthew F.; Myers, Matthew C.; Giuliani, John L.; Sethian, John D.; Burns, Patrick M.; Hegeler, Frank; Jaynes, Reginald

    2008-02-01

    Electra is a repetitively pulsed, electron beam pumped Krypton Fluoride (KrF) laser at the Naval Research Laboratory that is developing the technologies that can meet the Inertial Fusion Energy (IFE) requirements for durability, efficiency, and cost. The technologies developed on Electra should be directly scalable to a full size fusion power plant beam line. As in a full size fusion power plant beam line, Electra is a multistage laser amplifier system which, consists of a commercial discharge laser (LPX 305i, Lambda Physik), 175 keV electron beam pumped (40 ns flat-top) preamplifier, and 530 keV (100 ns flat-top) main amplifier. Angular multiplexing is used in the optical layout to provide pulse length control and to maximize laser extraction from the amplifiers. Single shot yield of 452 J has been extracted from the initial shots of the Electra laser system using a relatively low energy preamplifier laser beam. In rep-rate burst of 5 Hz for durations of one second a total energy of 1.585 kJ (average 317 J/pulse) has been attained. Total energy of 2.5 kJ has been attained over a two second period. For comparison, the main amplifier of Electra in oscillator mode has demonstrated at 2.5 Hz rep-rate average laser yield of 270 J over a 2 hour period.

  4. Comparison of automated repetitive-sequence-based polymerase chain reaction and spa typing versus pulsed-field gel electrophoresis for molecular typing of methicillin-resistant Staphylococcus aureus.

    Science.gov (United States)

    Church, Deirdre L; Chow, Barbara L; Lloyd, Tracie; Gregson, Daniel B

    2011-01-01

    Automated repetitive polymerase chain reaction (PCR) (DiversiLab, bioMérieux, St. Laurent, Quebec, Canada) and single locus sequence typing of the Staphylococcus protein A (spa) gene with spa-type assignment by StaphType RIDOM software were compared to pulsed-field gel electrophoresis (PFGE) as the "gold standard" method for methicillin-resistant Staphylococcus aureus (MRSA) typing. Fifty-four MRSA isolates were typed by all methods: 10 of known PFGE CMRSA type and 44 clinical isolates. Correct assignment of CMRSA type or cluster occurred for 47 of 54 (87%) of the isolates when using a rep-PCR similarity index (SI) of ≥95%. Rep-PCR gave 7 discordant results [CMRSA1 (3), CMRSA2 (1), CMRSA4 (1), and CMRSA10 (2)], and some CMRSA clusters were not distinguished (CMRSA10/5/9, CMRSA 7/8, and CMRSA3/6). Several spa types occurred within a single PFGE or repetitive PCR types among the 19 different spa types found. spa type t037 was shared by CMRSA3 and CMRSA6 strains, and CMRSA9 and most CMRSA10 strains shared spa type t008. Time to results for PFGE, repetitive PCR, and spa typing was 3-4 days, 24 h, and 48 h, respectively. The annual costs of using spa or repetitive PCR were 2.4× and 1.9× higher, respectively, than PFGE but routine use of spa typing would lower annual labor costs by 0.10 full-time equivalents compared to PFGE. Repetitive PCR is a good method for rapid outbreak screening, but MRSA isolates that share the same repetitive PCR or PFGE patterns can be distinguished by spa typing. Copyright © 2011 Elsevier Inc. All rights reserved.

  5. Rep-PCR--a variant to RAPD or an independent technique of bacteria genotyping? A comparison of the typing properties of rep-PCR with other recognised methods of genotyping of microorganisms.

    Science.gov (United States)

    Baldy-Chudzik, K

    2001-01-01

    The paper presents technical aspects of rep-PCR fingerprinting technique and compares its typing abilities, differentiation power and reproducibility with other recognised and recommended genotyping methods. Although rep-PCR fingerprinting is similar to MAAP techniques, it demonstrates some essentially different elements. The data presented in this review, indicate a rep-PCR genomic fingerprinting technique as a highly discriminating, independent screening method for determining the taxonomical diversity of bacterial population.

  6. Digital repetitive control under varying frequency conditions

    OpenAIRE

    Ramos Fuentes, Germán Andrés

    2012-01-01

    The tracking/rejection of periodic signals constitutes a wide field of research in the control theory and applications area and Repetitive Control has proven to be an efficient way to face this topic; however, in some applications the period of the signal to be tracked/rejected changes in time or is uncertain, which causes and important performance degradation in the standard repetitive controller. This thesis presents some contributions to the open topic of repetitive control workin...

  7. Repetition frequency scaling of an all-polarization maintaining erbium-doped mode-locked fiber laser based on carbon nanotubes saturable absorber

    Energy Technology Data Exchange (ETDEWEB)

    Sotor, J., E-mail: jaroslaw.sotor@pwr.edu.pl; Sobon, G.; Abramski, K. M. [Laser and Fiber Electronics Group, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw (Poland); Jagiello, J.; Lipinska, L. [Institute of Electronic Materials Technology, Wolczynska 133, 01-919 Warsaw (Poland)

    2015-04-07

    We demonstrate an all-polarization maintaining (PM), mode-locked erbium (Er)-doped fiber laser based on a carbon nanotubes (CNT) saturable absorber (SA). The laser resonator was maximally simplified by using only one passive hybrid component and a pair of fiber connectors with deposited CNTs. The repetition frequency (F{sub rep}) of such a cost-effective and self-starting mode-locked laser was scaled from 54.3 MHz to 358.6 MHz. The highest F{sub rep} was obtained when the total cavity length was shortened to 57 cm. The laser allows ultrashort pulse generation with the duration ranging from 240 fs to 550 fs. Because the laser components were based on PM fibers the laser was immune to the external perturbations and generated laniary polarized light with the degree of polarization (DOP) of 98.7%.

  8. [Repetition and fear of dying].

    Science.gov (United States)

    Lerner, B D

    1995-03-01

    In this paper a revision is made of the qualifications of Repetition (R) in Freuds work, i.e. its being at the service of the Pleasure Principle and, beyond it, the binding of free energy due to trauma. Freud intends to explain with this last concept the "fort-da" and the traumatic dreams (obsessively reiterated self-reproaches may be added to them). The main thesis of this work is that R. is not only a defense against the recollection of the ominous past (as in the metaphorical deaths of abandonment and desertion) but also a way of maintaining life and identify fighting against the inescapable omninous future (known but yet experienced), i.e. our own death. Some forms of R. like habits, identificatory behaviors and sometimes even magic, are geared to serve the life instinct. A literary illustration shows this desperate fight.

  9. Pressure rig for repetitive casting

    Science.gov (United States)

    Vasquez, Peter (Inventor); Hutto, William R. (Inventor); Philips, Albert R. (Inventor)

    1989-01-01

    The invention is a pressure rig for repetitive casting of metal. The pressure rig performs like a piston for feeding molten metal into a mold. Pressure is applied to an expandable rubber diaphragm which expands like a balloon to force the metal into the mold. A ceramic cavity which holds molten metal is lined with blanket-type insulating material, necessitating only a relining for subsequent use and eliminating the lengthy cavity preparation inherent in previous rigs. In addition, the expandable rubber diaphragm is protected by the insulating material thereby decreasing its vulnerability to heat damage. As a result of the improved design the life expectancy of the pressure rig contemplated by the present invention is more than doubled. Moreover, the improved heat protection has allowed the casting of brass and other alloys with higher melting temperatures than possible in the conventional pressure rigs.

  10. provincia de Buenos Aires (República Argentina

    Directory of Open Access Journals (Sweden)

    Rubén Alberto Cervini

    2008-01-01

    Full Text Available En el estudio que se informa se investigaron las relaciones entre progreso de aprendizaje (valor agregado, motivación y autoconcepto académico del alumno en Matemática, en el marco de los efectos de las características socioeconómicas y académicas del alumno y de su escuela. Para la medición del valor agregado se utilizaron los resultados de dos pruebas estandarizadas de Matemática aplicadas a una cohorte de alumnos de la Provincia de Buenos Aires (República Argentina en dos momentos diferentes (séptimo y noveno años de Educación General Bási ca - EGB. Las mediciones de motivación y de autoconcepto se basaron en respuestas a ítemes binarios de actitudes con respecto a Ma temática y fueron definidas utilizando el análisis de la componente prin - cipal, a partir de la matriz de correlación tetracórica (método heurístico. Para el análisis de las correlaciones se utilizó la téc nica estadística de modelos multinivel. El presente estudio relaciona los resultados empíricos con la teoría del habitus de Bourdieu. Inicialmente, el autoconcepto resulta significativa y positivamente asociado con el puntaje en la prueba y con el progreso de aprendizaje posterior. Este efecto continúa siendo significati vo aun cuando se controlan los efectos del nivel socioeconómi - co y del género del alumno. Sin embargo, su capacidad ex plicativa es muy tenue desde el punto de vista práctico. Si bien este resultado confirmaría la hipótesis central de la teoría de la reproducción, indica un sendero a través del cual podrían producirse transformaciones. Uno de los desafíos del sistema educativo es promover el habitus escolar cultivado (autoconcepto más allá de las determinaciones sociales, culturales y económicas.

  11. The oligomeric Rep protein of Mungbean yellow mosaic India virus (MYMIV) is a likely replicative helicase.

    Science.gov (United States)

    Choudhury, Nirupam Roy; Malik, Punjab Singh; Singh, Dharmendra Kumar; Islam, Mohammad Nurul; Kaliappan, Kosalai; Mukherjee, Sunil Kumar

    2006-01-01

    Geminiviruses replicate by rolling circle mode of replication (RCR) and the viral Rep protein initiates RCR by the site-specific nicking at a conserved nonamer (TAATATT downward arrow AC) sequence. The mechanism of subsequent steps of the replication process, e.g. helicase activity to drive fork-elongation, etc. has largely remained obscure. Here we show that Rep of a geminivirus, namely, Mungbean yellow mosaic India virus (MYMIV), acts as a replicative helicase. The Rep-helicase, requiring > or =6 nt space for its efficient activity, translocates in the 3'-->5' direction, and the presence of forked junction in the substrate does not influence the activity to any great extent. Rep forms a large oligomeric complex and the helicase activity is dependent on the oligomeric conformation ( approximately 24mer). The role of Rep as a replicative helicase has been demonstrated through ex vivo studies in Saccharomyces cerevisiae and in planta analyses in Nicotiana tabacum. We also establish that such helicase activity is not confined to the MYMIV system alone, but is also true with at least two other begomoviruses, viz., Mungbean yellow mosaic virus (MYMV) and Indian cassava mosaic virus (ICMV).

  12. Physics and medicine: at Erice the new perspectives of an ancient cooperation

    CERN Multimedia

    2004-01-01

    From April the 15th to the 17th, at Ettore Majorana Foundation in Erice, in the context of the International School on Physics and Industry, there will be a congress entitled "Particle accelerators and detectors: from physics to medicine".

  13. Helping Children Deal with Trauma and Terrorism. ERIC/EECE Report.

    Science.gov (United States)

    Cesarone, Bernard

    2002-01-01

    Summarizes selected ERIC documents, journal articles, and World Wide Web resources that discuss ways to help children deal with traumatic events, both natural and human-caused, including the effects of terrorism. (DLH)

  14. 全能吉他手——Eric Johnson

    Institute of Scientific and Technical Information of China (English)

    Bird Hey

    2012-01-01

    目前,Eric Johnson正在酝酿一张名为《旋风计划》的专辑。参与者有世界知名鼓手杰尔·马罗塔和知名贝司手兼吉他手崔·耿。杰尔·马罗他不仅是鼓手,而且是制作人,曾和彼得·加布里尔、保罗·麦卡特尼、爱尔维斯·卡斯特罗、“印地哥女孩”乐队、“恐惧眼泪”乐队、“奥提斯大厦”乐队和琼·阿马楚丁等大腕合作过。

  15. Comparing repetition-based melody segmentation models

    NARCIS (Netherlands)

    Rodríguez López, M.E.; de Haas, Bas; Volk, Anja

    2014-01-01

    This paper reports on a comparative study of computational melody segmentation models based on repetition detection. For the comparison we implemented five repetition-based segmentation models, and subsequently evaluated their capacity to automatically find melodic phrase boundaries in a corpus of 2

  16. Task Repetition and Second Language Speech Processing

    Science.gov (United States)

    Lambert, Craig; Kormos, Judit; Minn, Danny

    2017-01-01

    This study examines the relationship between the repetition of oral monologue tasks and immediate gains in L2 fluency. It considers the effect of aural-oral task repetition on speech rate, frequency of clause-final and midclause filled pauses, and overt self-repairs across different task types and proficiency levels and relates these findings to…

  17. Repetitions: A Cross-Cultural Study.

    Science.gov (United States)

    Murata, Kumiko

    1995-01-01

    This study investigated how repetition is used in conversation among native speakers of British English, native speakers of Japanese, and Japanese speakers of English. Five interactional functions of repetition (interruption-orientated, solidarity, silence-avoidance, hesitation, and reformulation) were identified, as well as the cultural factors…

  18. Radioisotope Electric Propulsion (REP): A Near-Term Approach to Nuclear Propulsion

    Science.gov (United States)

    Schmidt, George R.; Manzella, David H.; Kamhawi, Hani; Kremic, Tibor; Oleson, Steven R.; Dankanich, John W.; Dudzinski, Leonard A.

    2009-01-01

    Studies over the last decade have shown radioisotope-based nuclear electric propulsion to be enhancing and, in some cases, enabling for many potential robotic science missions. Also known as radioisotope electric propulsion (REP), the technology offers the performance advantages of traditional reactor-powered electric propulsion (i.e., high specific impulse propulsion at large distances from the Sun), but with much smaller, affordable spacecraft. Future use of REP requires development of radioisotope power sources with system specific powers well above that of current systems. The US Department of Energy and NASA have developed an advanced Stirling radioisotope generator (ASRG) engineering unit, which was subjected to rigorous flight qualification-level tests in 2008, and began extended lifetime testing later that year. This advancement, along with recent work on small ion thrusters and life extension technology for Hall thrusters, could enable missions using REP sometime during the next decade.

  19. Spreading the Eco-Message: Using Proactive Coping to Aid Eco-Rep Behavior Change Programming

    Directory of Open Access Journals (Sweden)

    Matthew J. Zawadzki

    2013-04-01

    Full Text Available Making pro-environmental behavior changes can be difficult, particularly when these changes challenge daily routines and comfortable lifestyles. We designed and implemented an eco-representative intervention program to help students reduce their energy use by proactively coping with barriers to pro-environmental behavior change, and then communicate effective behavior change strategies to student peers. Twenty-nine first-year college students participated in a four-week proactive coping training to change five environmentally impactful behaviors and then spread behavior change messages to fellow residents during a two-week energy challenge. Eco-reps successfully changed their own behaviors in a pro-environmental direction by generating important barriers and successful facilitators for behavior change, and eco-rep residence halls were more likely to reduce energy and maintain reductions compared to non-eco-rep halls. Implications for future environmental behavior change interventions are discussed.

  20. DIMENSION VARIATION OF FEATURE-BASED MODEL BY OPERATING DIRECTLY ON B-REP

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    A new algorithm for dimension variation of feature-based models is developed.The algorithm is based on B-Rep/CSG hybrid scheme and operates directly on B-Rep.Product information (including the features locating dimensions and other data for manufacture) will not lose after model variation and modification.Furthermore, the definition and solution of features constraints are also supported.The scheme of directly operating on B-Rep overcomes many drawbacks of other proposed methods, most of which need to redo all of the previous work to implement dimension variation, so the computational cost is expensive and product information will lose.What is presented in this paper makes a new way in the area of parametric design.

  1. Digital repetitive control under varying frequency conditions

    CERN Document Server

    Ramos, Germán A; Olm, Josep M

    2013-01-01

    The tracking/rejection of periodic signals constitutes a wide field of research in the control theory and applications area. Repetitive Control has proven to be an efficient way to face this topic. However, in some applications the frequency of the reference/disturbance signal is time-varying or uncertain. This causes an important performance degradation in the standard Repetitive Control scheme. This book presents some solutions to apply Repetitive Control in varying frequency conditions without loosing steady-state performance. It also includes a complete theoretical development and experimental results in two representative systems. The presented solutions are organized in two complementary branches: varying sampling period Repetitive Control and High Order Repetitive Control. The first approach allows dealing with large range frequency variations while the second allows dealing with small range frequency variations. The book also presents applications of the described techniques to a Roto-magnet plant and...

  2. Strategies for Using Repetition as a Powerful Teaching Tool

    Science.gov (United States)

    Saville, Kirt

    2011-01-01

    Brain research indicates that repetition is of vital importance in the learning process. Repetition is an especially useful tool in the area of music education. The success of repetition can be enhanced by accurate and timely feedback. From "simple repetition" to "repetition with the addition or subtraction of degrees of freedom," there are many…

  3. Strategies for Using Repetition as a Powerful Teaching Tool

    Science.gov (United States)

    Saville, Kirt

    2011-01-01

    Brain research indicates that repetition is of vital importance in the learning process. Repetition is an especially useful tool in the area of music education. The success of repetition can be enhanced by accurate and timely feedback. From "simple repetition" to "repetition with the addition or subtraction of degrees of freedom," there are many…

  4. Recuperar la història de la Segona República Espanyola

    OpenAIRE

    Fontana, Josep, 1931-

    2003-01-01

    L'article recull un discurs de Josep Fontana pronunciat l'octubre de 2002 a la Universitat Autònoma de Barcelona on reflexiona entorn la necessitat de formular una nova lectura història de la Segona República espanyola, de la guerra civil i del franquisme. El artículo recoge un discurso de Josep Fontana pronunciado en octubre del 2002 en la Universitat Autònoma de Barcelona donde reflexiona acerca de la necesidad de formular una nueva lectura de la historia de la Segunda República española...

  5. Design of a Battery Intermediate Storage System for Rep-Rated Pulsed Power Loads

    Science.gov (United States)

    2013-04-01

    Abstract—The U.S. Naval Research Laboratory (NRL) is developing a battery-powered, rep-rate charger for a 60-kJ capacitor bank . The capacitor ...developing a rapid charger for a 60-kJ capacitor bank capable of charging a 4800- µF capacitor to 5-kV in roughly five seconds. This system needs to...U.S. Naval Research Laboratory (NRL) is developing a battery-powered, rep-rate charger for a 60-kJ capacitor bank . The capacitor will be charged with

  6. El derecho del trabajo democrático en la República de Weimar

    OpenAIRE

    Gil Albuerquerque, Román de la Trinidad

    2016-01-01

    La tesis "El Derecho del Trabajo democrático en la República de Weimar" es, de una parte, una introducción contextualizadora a la historia político-social, sindical, del Derecho del Trabajo y de la obra de significados juristas demócratas comprometidos con el nacimiento de la nueva disciplina jurídica (y en parte responsables de su constitucionalización) y con su desarrollo en la República de Weimar, así como una consideración transversal y comparativa de varias instituciones fundamentales p...

  7. El derecho del trabajo democrático en la República de Weimar

    OpenAIRE

    Gil Albuerquerque, Román de la Trinidad

    2016-01-01

    La tesis "El Derecho del Trabajo democrático en la República de Weimar" es, de una parte, una introducción contextualizadora a la historia político-social, sindical, del Derecho del Trabajo y de la obra de significados juristas demócratas comprometidos con el nacimiento de la nueva disciplina jurídica (y en parte responsables de su constitucionalización) y con su desarrollo en la República de Weimar, así como una consideración transversal y comparativa de varias instituciones fundamentales p...

  8. Repetition priming from moving faces.

    Science.gov (United States)

    Lander, Karen; Bruce, Vicki

    2004-06-01

    Recent experiments have suggested that seeing a familiar face move provides additional dynamic information to the viewer, useful in the recognition of identity. In four experiments, repetition priming was used to investigate whether dynamic information is intrinsic to the underlying face representations. The results suggest that a moving image primes more effectively than a static image, even when the same static image is shown in the prime and the test phases (Experiment 1). Furthermore, when moving images are presented in the test phase (Experiment 2), there is an advantage for moving prime images. The most priming advantage is found with naturally moving faces, rather than with those shown in slow motion (Experiment 3). Finally, showing the same moving sequence at prime and test produced more priming than that found when different moving sequences were shown (Experiment 4). The results suggest that dynamic information is intrinsic to the face representations and that there is an advantage to viewing the same moving sequence at prime and test.

  9. 2008-2012年上海市肠炎沙门氏菌分离株毒力基因筛查与ERIC-PCR分型%Screening of Virulence Genes and ERIC-PCR Sub-typing for Salmonella Enteritidis Isolates in Shanghai during 2008-2012

    Institute of Scientific and Technical Information of China (English)

    庄孝飞; 周秀娟; 许学斌; 史贤明; 施春雷

    2015-01-01

    采集上海地区2008-2012年来自临床病人和市售食品的肠炎沙门氏菌分离株90株;采用聚合酶链式反应(polymerase chain reaction,PCR)方法,对存在于毒力岛SPI和毒力质粒上的9种常见毒力基因携带情况进行了调查;并采用本实验优化的肠杆菌基因间保守重复序列-聚合酶链式反应(enterobacterial repetitive intergenic consensus-PCR,ERIC-PCR)方法对这些分离株进行亚分型.结果显示:毒力基因invH、sopE、sugR的携带率为100.0% (90/90),iacP、avrA、rhuM、prgK均为98.9% (89/90),而spvB、spvC分别为85.6% (77/90)和78.9%(71/90).优化的ERIC-PCR体系能够较好地区分8种主要沙门氏菌血清型,共17株菌,辛普森指数(D值)为0.970 6.然而,90株肠炎沙门氏菌分离株却具有一致的ERIC-PCR指纹图谱.在所调查分离株中,毒力基因的携带率较高,尤其是invH、sopE和sugR,对人类健康存在较大威胁;ERIC-PCR虽然可用于区分沙门氏菌不同血清型,但对肠炎沙门氏菌的分型效果不佳.

  10. Anthropometry increases 1 repetition maximum predictive ability of NFL-225 test for Division IA college football players.

    Science.gov (United States)

    Hetzler, Ronald K; Schroeder, Brian L; Wages, Jennifer J; Stickley, Christopher D; Kimura, Iris F

    2010-06-01

    The purpose of this study was to compare existing 1 repetition maximum (1RM) bench press prediction equations in National Collegiate Athletic Association (NCAA) Division IA college football players and determine if the error associated with the prediction of 1RM bench press from the National Football League (NFL)-225 test could be reduced through the addition of anthropometric measurements. Anthropometric measures, 1RM bench press, NFL-225 test repetitions to fatigue, and body composition data were collected on 87 Division IA football players (mean+/-SD age 19.9+/-1.3 years; height 182.3+/-7.3 cm; body mass 102.3+/-21.1 kg; % fat 13.9+/-6.7; 1RM bench press 140.5+/-2 6.6 kg; and NFL-225 reps to fatigue 14.1+/-8.0). Hierarchical regression revealed an R=0.87 when predicting 1RM from the NFL-225 test alone, which improved to R=0.90 with the addition of the anthropometric variables: arm circumference and arm length. The following equation was the best performing model to predict 1RM bench press: 1RM (lb)=299.08+2.47 arm circumference (cm)--4.60 arm length (cm)+5.84 reps @ 225; SEE=18.3 lb). This equation predicted 43.7% of subjects' within +/-10 lb of their actual 1RM bench press. Using a crossvalidation group, the equation resulted in estimates of 1RM which were not significantly different than the actual 1RM. Because of the variability that has been shown to be associated with 1RM prediction equations, the use of actual 1RM testing is recommended when this is a critical variable. However, coaches, scouts, and athletes, who choose to estimate 1RM bench press using repetitions to failure from the NFL-225 test, may benefit from the use of the equations developed in this study to estimate 1RM bench press with the inclusion of simple anthropometric measurements.

  11. 产碳青霉烯酶产气肠杆菌的ERIC-PCR图谱分析%Map analysis of ERIC-PCR of carbapenemases-producing enterobacter aerogenes

    Institute of Scientific and Technical Information of China (English)

    刘瑛; 费奇力; 陈峰; 陶晓勤; 刁文晶; 沈立松

    2011-01-01

    目的 了解医院临床产碳青霉烯酶产气肠杆菌感染流行情况,为医院感染控制提供帮助.方法 对医院检验科微生物实验室在2009年6月-2010年3月临床分离出的16株产碳青霉烯酶产气肠杆菌及1株非产碳青霉烯酶产气肠杆菌,用肠杆菌基因间重复一致序列聚合酶链反应(ERIC-PCR)进行基因分型.结果 17株菌株分为两个克隆型,其中16株产碳青霉烯酶产气肠杆菌为同一型,而另1株非产酶菌株为另一型.结论 医院存在产碳青霉烯酶产气肠杆菌克隆传播现象,医护人员应予以高度关注,并及时采取有效措施.%OBJECTIVE To understand the infection prevalence of carbapenemases-producing Enterobacter aerogenes in hospital and provide supports for antibiotic policy and infection control. METHODS A total of 16 strains of carbapenems-producing E. aerogenes and 1 strain of E. aerogene were isolated from clinical laboratory of microbiology from Jun 2009 to Mar 2010. ERIC(Enterobacterial Repetitive Intergenic Consensus)-PCR was used to analyze the molecular epidemiology of the 17 isolates. RESULTS Totally 17 isolates were classified into two genotypes, in which 16 isolates were the same type, 1 isolate was another type. CONCLUSION The results suggest that patients in ICU and the emergency ward exist the prevalence between carbapenemases-producing E.aerogenes. Health care workers should emphasis on this phenomenon and take effective measures.

  12. 75 FR 32493 - NUREG-0654/FEMA-REP-, Rev. 1, Supplement 3, Guidance for Protective Action Recommendations for...

    Science.gov (United States)

    2010-06-08

    ... SECURITY Federal Emergency Management Agency NUREG-0654/FEMA-REP-, Rev. 1, Supplement 3, Guidance for... be held to discuss the proposed Supplement 3 to NUREG-0654/FEMA-REP-1, Rev.1, Guidance for Protective Action Recommendations for General Emergencies (NUREG Supp 3). NUREG Supp 3 addresses onsite and...

  13. Precision markedly attenuates repetitive lift capacity.

    Science.gov (United States)

    Collier, Brooke R; Holland, Laura; McGhee, Deirdre; Sampson, John A; Bell, Alison; Stapley, Paul J; Groeller, Herbert

    2014-01-01

    This study investigated the effect of precision on time to task failure in a repetitive whole-body manual handling task. Twelve participants were required to repetitively lift a box weighing 65% of their single repetition maximum to shoulder height using either precise or unconstrained box placement. Muscle activity, forces exerted at the ground, 2D body kinematics, box acceleration and psychophysical measures of performance were recorded until task failure was reached. With precision, time to task failure for repetitive lifting was reduced by 72%, whereas the duration taken to complete a single lift and anterior deltoid muscle activation increased by 39% and 25%, respectively. Yet, no significant difference was observed in ratings of perceived exertion or heart rate at task failure. In conclusion, our results suggest that when accuracy is a characteristic of a repetitive manual handling task, physical work capacity will decline markedly. The capacity to lift repetitively to shoulder height was reduced by 72% when increased accuracy was required to place a box upon a shelf. Lifting strategy and muscle activity were also modified, confirming practitioners should take into consideration movement precision when evaluating the demands of repetitive manual handling tasks.

  14. RiskREP: Risk-Based Security Requirements Elicitation and Prioritization (extended version)

    NARCIS (Netherlands)

    Herrmann, Andrea; Morali, A.

    2010-01-01

    Today, companies are required to be in control of the security of their IT assets. This is especially challenging in the presence of limited budgets and conflicting requirements. Here, we present Risk-Based Requirements Elicitation and Prioritization (RiskREP), a method for managing IT security

  15. Overview of RepLab 2014: Author Profiling and Reputation Dimensions for Online Reputation Management

    NARCIS (Netherlands)

    Amigó, E.; Carrillo-de-Albornoz, J.; Chugur, I.; Corujo, A.; Gonzalo, J.; Meij, E.; de Rijke, M.; Spina, D.

    2014-01-01

    This paper describes the organisation and results of RepLab 2014, the third competitive evaluation campaign for Online Reputation Management systems. This year the focus lied on two new tasks: reputation dimensions classification and author profiling, which complement the aspects of reputation

  16. Romanos e Partos: atividades bélicas na República e no Principado

    OpenAIRE

    Ana Teresa Marques Gonçalves

    2005-01-01

    O objetivo deste artigo é analisar algumas das várias batalhas travadas entre romanos e partos ao longo da República e do Principado, utilizando para tanto as obras de Plutarco, Dion Cássio, Herodiano, Polieno, Eutrópio, Aurélio Victor e Zózimo.

  17. RePS: a sequence assembler that masks exact repeats identified from the shotgun data

    DEFF Research Database (Denmark)

    Wang, Jun; Wong, Gane Ka-Shu; Ni, Peixiang;

    2002-01-01

    We describe a sequence assembler, RePS (repeat-masked Phrap with scaffolding), that explicitly identifies exact 20mer repeats from the shotgun data and removes them prior to the assembly. The established software is used to compute meaningful error probabilities for each base. Clone-end-pairing i...

  18. Simulace dynamiky vozidla využitím software V-REP

    OpenAIRE

    2016-01-01

    Tato práce se zabývá modelováním dynamiky a vizualizací experimentálního vozidla CAR4 v programu V-REP. V první části se práce zabývá představením a možnostmi programu V-REP. V další části je realizováno modelování vozidla CAR4 s následným řízením. K řízení je využit program V-REP a Matlab. V poslední fázi proběhlo testování modelu v simulaci. This theasis deals with modeling dynamics and visualization of experimental vehicle CAR4 in software V-REP. The first part shows introduction with V...

  19. Dictadura, democracia, república: Un análisis conceptual

    OpenAIRE

    Peña, Lorenzo

    2009-01-01

    Conferencia magistral sobre los conceptos de democracia, dictadura y república. "Memoria del Primer Encuentro Internacional sobre el poder en el pasado y el presente de América Latina" coord. por Francisco Lizcano y Guadalupe Zamudio Toluca: Universidad Autónoma del Estado de México, 2009 pp. 29-60. ISBN 978-607-422-069-8.

  20. ERIC Documents on Foreign Language Teaching and Linguistics: List Number 15. CAL-ERIC/CLL Series on Languages and Linguistics, No. 38.

    Science.gov (United States)

    Eddy, Peter, Comp.; And Others

    1976-01-01

    This is the fifteenth in a series of catalogues of ERIC documents of interest to teachers and researchers in foreign languages and linguistics. The documents cited in the present list appeared in "Resources in Education" from January through June 1975. Titles are listed under the following headings and subheadings: (1) general: administration,…

  1. Repetitive Bibliographical Information in Relational Databases.

    Science.gov (United States)

    Brooks, Terrence A.

    1988-01-01

    Proposes a solution to the problem of loading repetitive bibliographic information in a microcomputer-based relational database management system. The alternative design described is based on a representational redundancy design and normalization theory. (12 references) (Author/CLB)

  2. Computer-Related Repetitive Stress Injuries

    Science.gov (United States)

    ... on the shoulder Epicondylitis: elbow soreness often called "tennis elbow" Ganglion cyst: swelling or lump in the wrist ... Bones, Muscles, and Joints Carpal Tunnel Syndrome Medial Epicondylitis Repetitive Stress Injuries Contact Us Print Resources Send ...

  3. Digital repetitive control under varying frequency conditions

    OpenAIRE

    Ramos Fuentes, Germán Andrés

    2012-01-01

    Premi extraordinari doctorat curs 2011-2012, àmbit d’Enginyeria Industrial The tracking/rejection of periodic signals constitutes a wide field of research in the control theory and applications area and Repetitive Control has proven to be an efficient way to face this topic; however, in some applications the period of the signal to be tracked/rejected changes in time or is uncertain, which causes and important performance degradation in the standard repetitive controller. This the...

  4. Eric Hermelin and mystical hermeneutics: interpretation beyond itself

    Directory of Open Access Journals (Sweden)

    Tina Hamrin

    1999-01-01

    Full Text Available Eric Hermelin (1860-1944 translated more than 10.000 pages Persian Sufi poetry, German texts by Jakob Böhme and Latin texts by Emmanuel Swedenborg. In the translation process Hermelin shaped his strategies and perceptions in ways which served their patterns of desire, and what they constructed reflected and served their own unique identity. Added up, the devices which constitute strategies of reading set in motion by the selfs defense of its identity are: Defenses, Expectations, Fantasies, and Transformations. Hermelin's mystical hermeneutics produced not only understanding of texts, but often no less produced an increased awareness, with appropriate hermeneutical sensitivity, of self-perception and self-identity. A self-awareness and a strengthening of an individual and corporate identity as one who has a stake in the texts and that to which they bear witness constitutes an important reader-effect in this case of Sufi poetry. But without any principle of suspicion, in Gadamer's terminology a premature fusion of horizons will take place before Hermelin has listened in openness with respect for the tension between the horizons of the text and the horizon of Hermelin. The textual horizon has col-lapsed into that of Hermelin's narrative biography, and is unable to do more than to speak back his own valnes and desires. Something socio-pragmatical was after all woven into the controversial bon vivant, when he used literary works to replicate himself. At the same time, the texts spoke from beyond the self.

  5. Eric Hobsbawm: A historian in search of the future

    Directory of Open Access Journals (Sweden)

    Subotić Milan

    2009-01-01

    Full Text Available In this paper the author interpreted the historiographic span of work of Eric Hobsbawm, one of the most famous and most influential contemporary historians. Bearing in mind the extensiveness and complexity of this span of work, the author limited his analysis to two theoretical problems - Hobsbawm's view of the concept of 'social history' and the exploration of the function of 'historical materialism' in the emergence and realisation of this concept. In the author's opinion, Marxism served Hobsbawm as a productive theoretical standpoint in the thematisation of the history of the 'long 19th century', but it prevented him from understanding more fully the 'short 20th century' (The Age of Extremes. The basic reason for this lies in the fact that the 'Soviet experiment' which in essence defined the 20th century cannot be explained from the 'historical-materialistic' perspective. It is precisely in the theoretical deficits of the Marxist theoretical paradigm, and not in Hobsbawm's political bias, that the author identifies the basic cause for the weakness of his interpretation of the epoch of Communism. For this reason, in the final part of the paper the author formulated the thesis on the necessity of formulating a new theoretical framework and conceptual apparatus which would provide us not only with a better understanding of the past, but of the present and the future as well.

  6. Fans of Columbine shooters Eric Harris and Dylan Klebold

    Directory of Open Access Journals (Sweden)

    Andrew Ryan Rico

    2015-09-01

    Full Text Available On April 20, 1999, Eric Harris and Dylan Klebold murdered 12 students and one teacher at Columbine High School in Littleton, Colorado, in what was then the deadliest school shooting in American history. Despite causing a national panic and serving as a flash point for larger narratives on bullying, gun control, and media violence, both boys have gained active online fans. These fandoms dedicated to the Columbine shooters are widely referred to as dark examples of Internet communities, while the fans are also frequently denigrated as unstable and violent outcasts. Such dark online fandoms are yet to permeate mainstream culture or to challenge the preexisting perception of these communities as breeding grounds for the next wave of school shooters. While studies have covered the types of fans and their myriad interests, the field remains focused on more conventional examples of fan communities. In an effort to challenge and expand the object of focus when we study fandom, this qualitative study examines Columbine fans and their activity in order to understand the dominant motives they appear to have for engaging with and around such controversial figures and then concludes by exploring how this community might help us reflect more broadly on our concept of fandom. Redeeming these fans as part of diverse and complex communities of social relevance can demonstrate how even a dark fandom such as that of these Columbine shooters provides valuable cultural insights and benefits the field of fan studies.

  7. A QUESTÃO ESTATISMO HEGELIANO SEGUNDO ERIC WEIL

    Directory of Open Access Journals (Sweden)

    Daniel Benevides Soares

    2013-06-01

    Full Text Available A visão de Hegel como um pensador conservador não é um fenômeno isolado. Para alguns críticos, Hegel é comumente considerado um apologeta do Estado prussiano e um filósofo daquilo que comumente se denomina estatismo. Eric Weil, contudo, não considera essa definição como condizente com uma retratação fiel do filósofo alemão, assemelhando-se mais a uma caricatura. Nesse sentido, Weil defende uma leitura do pensamento político hegeliano que põe em xeque essa visão, fazendo uma crítica da crítica que, tal como Kant é o rigorista moral, ou Platão é o filósofo das Idéias, coloca Hegel como o filósofo do estatismo e para quem o indivíduo é nada e o Estado é tudo. Para determinar até que ponto essa perspectiva possui lastro, Weil advoga em favor de uma análise do pensamento político de Hegel, inquirindo sobre a real dimensão que o Estado possui no seio desse pensamento. É dessa maneira que é possível delimitar até que ponto esse retrato comumente traçado de Hegel corresponde com a realidade.

  8. Pre-amyloid oligomers of the proteotoxic RepA-WH1 prionoid assemble at the bacterial nucleoid

    Science.gov (United States)

    Moreno-del Álamo, María; de la Espina, Susana Moreno-Díaz; Fernández-Tresguerres, M. Elena; Giraldo, Rafael

    2015-01-01

    Upon binding to short specific dsDNA sequences in vitro, the N-terminal WH1 domain of the plasmid DNA replication initiator RepA assembles as amyloid fibres. These are bundles of single or double twisted tubular filaments in which distorted RepA-WH1 monomers are the building blocks. When expressed in Escherichia coli, RepA-WH1 triggers the first synthetic amyloid proteinopathy in bacteria, recapitulating some of the features of mammalian prion diseases: it is vertically transmissible, albeit non-infectious, showing up in at least two phenotypically distinct and interconvertible strains. Here we report B3h7, a monoclonal antibody specific for oligomers of RepA-WH1, but which does not recognize the mature amyloid fibres. Unlike a control polyclonal antibody generated against the soluble protein, B3h7 interferes in vitro with DNA-promoted or amyloid-seeded assembly of RepA-WH1 fibres, thus the targeted oligomers are on-pathway amyloidogenic intermediates. Immuno-electron microscopy with B3h7 on thin sections of E. coli cells expressing RepA-WH1 consistently labels the bacterial nucleoid, but not the large cytoplasmic aggregates of the protein. This observation points to the nucleoid as the place where oligomeric amyloid precursors of RepA-WH1 are generated, and suggests that, once nucleated by DNA, further growth must continue in the cytoplasm due to entropic exclusion. PMID:26423724

  9. Indexing for ERIC. Volume 2, Lesson 1-Introduction to Indexing; Lesson 2-How to Index a Document.

    Science.gov (United States)

    Langridge, D.W.; And Others

    This volume contains the first two lessons of a course in subject indexing based upon the Educational Resources Information Center (ERIC) Thesaurus, Lesson 1 is an introduction to indexing, containing definitions and concepts related to the theory of indexing and information retrieval as well as an introduction to the ERIC Thesaurus. Lesson 2 is…

  10. 溃疡性结肠炎肠道菌群结构ERIC-PCR指纹图谱分析%Itestinal flora structural features in the ulcerative colitis by ERIC-PCR fingerprinting

    Institute of Scientific and Technical Information of China (English)

    吴瑞丽; 孙自勤; 魏志

    2012-01-01

    目的 建立溃疡性结肠炎( ulcerative colitis,UC)与正常人的肠道细菌DNA指纹图谱,分析其肠道菌群的整体差异,并探讨UC患者肠道菌群的变化与疾病的发生、发展关系,为临床治疗UC提供理论依据.方法 收集55例UC(30例活动期,25例缓解期)患者及15例正常人粪便标本,提取总基因组DNA,应用ERIC(enterobacterial repetitive intergenic consensus,肠道细菌基因间重复序列)-PCR技术建立各组肠道细菌DNA指纹图谱,运用UVP凝胶电泳分析软件统计分析活动期UC、缓解期UC及正常对照者肠道菌群的多样性和相似性.结果 活动期UC患者肠道菌群结构多样性指数H3=2.2080,而缓解期UC和健康人肠道菌群结构多样性指数分别为2.7848、2.8080,且活动期UC菌群丰富度较其他两组低(P1.3<0.001,P2.3<0.001),而缓解期UC与正常对照组无明显区别(P1.2=0.542).结论 UC活动期患者肠道菌群结构多样性较UC缓解期、健康人肠道菌群结构存在一定程度的差异.肠道菌群失调可能是UC发病的一个重要因素.%Objective To establish DNA fingerprint of intestinal flora in patients with and without ulcerative colitis and then analyze the structural characteristics of those fingerprint. To investigate the role of the intestinal floar chang in the ulcerative colitis, and provide the basis for clinical treatment of UC. Methods We collected 55 cases UC patients, including UC active 30 cases and UC remission 25 cases, and 15 healthy peoples as control. The total DNA was extracted from the fecal samples, and enterobacterial repetitive intergenic consensus sequence polymerase chain reaction ( ERIC-PCR) was used to set up the DNA fingerprint of intestinal flora. UVP gel electrophoresis analysis software was used to assess the diversity and similarity among these 3 groups which were the UC active, UC remission and normal controls. Results The UC active' s Shannon-Weiner index was 2. 2080, but UC remission' s Shannon

  11. RepSeq – A database of amino acid repeats present in lower eukaryotic pathogens

    Directory of Open Access Journals (Sweden)

    Smith Deborah F

    2007-04-01

    Full Text Available Abstract Background Amino acid repeat-containing proteins have a broad range of functions and their identification is of relevance to many experimental biologists. In human-infective protozoan parasites (such as the Kinetoplastid and Plasmodium species, they are implicated in immune evasion and have been shown to influence virulence and pathogenicity. RepSeq http://repseq.gugbe.com is a new database of amino acid repeat-containing proteins found in lower eukaryotic pathogens. The RepSeq database is accessed via a web-based application which also provides links to related online tools and databases for further analyses. Results The RepSeq algorithm typically identifies more than 98% of repeat-containing proteins and is capable of identifying both perfect and mismatch repeats. The proportion of proteins that contain repeat elements varies greatly between different families and even species (3–35% of the total protein content. The most common motif type is the Sequence Repeat Region (SRR – a repeated motif containing multiple different amino acid types. Proteins containing Single Amino Acid Repeats (SAARs and Di-Peptide Repeats (DPRs typically account for 0.5–1.0% of the total protein number. Notable exceptions are P. falciparum and D. discoideum, in which 33.67% and 34.28% respectively of the predicted proteomes consist of repeat-containing proteins. These numbers are due to large insertions of low complexity single and multi-codon repeat regions. Conclusion The RepSeq database provides a repository for repeat-containing proteins found in parasitic protozoa. The database allows for both individual and cross-species proteome analyses and also allows users to upload sequences of interest for analysis by the RepSeq algorithm. Identification of repeat-containing proteins provides researchers with a defined subset of proteins which can be analysed by expression profiling and functional characterisation, thereby facilitating study of pathogenicity

  12. The Prevalence and Phenomenology of Repetitive Behavior in Genetic Syndromes

    Science.gov (United States)

    Moss, Joanna; Oliver, Chris; Arron, Kate; Burbidge, Cheryl; Berg, Katy

    2009-01-01

    We investigated the prevalence and phenomenology of repetitive behavior in genetic syndromes to detail profiles of behavior. The Repetitive Behaviour Questionnaire (RBQ) provides fine-grained identification of repetitive behaviors. The RBQ was employed to examine repetitive behavior in Angelman (N = 104), Cornelia de Lange (N = 101), Cri-du-Chat…

  13. Saladusi mäletavad pildid : järelvaade Eric Soovere fotograafilisele dokumentalistikale / Peeter Linnap

    Index Scriptorium Estoniae

    Linnap, Peeter, 1960-

    2009-01-01

    Ameerikas elanud eesti fotograafi Eric Soovere (sünd. Erich Soeson, 1916-2008) eluloolisi andmeid. Tema loomingust, mis jaguneb kolme perioodi: etnograafiline fotokooslus Lõuna-Eesti külaelanikest (1930ndate lõpp), Sooverede perekonna põgenemisteekond Eestist USA-sse (ca 1944-1949), perekonna elu Cincinnatis USA-s (1950ndad). Fotod eestlaste põgenemisteekonnast on avaldatud raamatus Eric Soovere. Käru ja kaameraga : pilte ja päevikulehti põgenemisteelt 1944-1949. Tallinn : Olion, 1999

  14. Professor Eric Can't See: A Project-Based Learning Case for Neurobiology Students.

    Science.gov (United States)

    Ogilvie, Judith Mosinger; Ribbens, Eric

    2016-01-01

    "Professor Eric Can't See" is a semi-biographical case study written for an upper level undergraduate Neurobiology of Disease course. The case is integrated into a unit using a project-based learning approach to investigate the retinal degenerative disorder Retinitis pigmentosa and the visual system. Some case study scenes provide specific questions for student discussion and problem-based learning, while others provide background for student inquiry and related active learning exercises. The case was adapted from "'Chemical Eric' Can't See," and could be adapted for courses in general neuroscience or sensory neuroscience.

  15. Saladusi mäletavad pildid : järelvaade Eric Soovere fotograafilisele dokumentalistikale / Peeter Linnap

    Index Scriptorium Estoniae

    Linnap, Peeter, 1960-

    2009-01-01

    Ameerikas elanud eesti fotograafi Eric Soovere (sünd. Erich Soeson, 1916-2008) eluloolisi andmeid. Tema loomingust, mis jaguneb kolme perioodi: etnograafiline fotokooslus Lõuna-Eesti külaelanikest (1930ndate lõpp), Sooverede perekonna põgenemisteekond Eestist USA-sse (ca 1944-1949), perekonna elu Cincinnatis USA-s (1950ndad). Fotod eestlaste põgenemisteekonnast on avaldatud raamatus Eric Soovere. Käru ja kaameraga : pilte ja päevikulehti põgenemisteelt 1944-1949. Tallinn : Olion, 1999

  16. práctica del turismo rural en la República de Cuba

    Directory of Open Access Journals (Sweden)

    Isis Alejandra Camargo Toribio

    2005-01-01

    Full Text Available El presente trabajo incluye parte de los resultados que se obtuvieron en la tesis doctoral “Aportaciones metodológicas para la implementación del turismo rural en la República de Cuba. Estudio de caso: zona turística de Viñales” (Camargo, 2003. El objetivo principal de este artículo es conocer las preferencias de los clientes internacionales para la práctica del turismo rural, modalidad que no está establecida de forma consciente en la República de Cuba y que, de acuerdo con el potencial naturalcultural existente, tiene amplias posibilidades para su introducción. Con este fin se aplica una encuesta donde los viajeros evalúan, en una escala ascendente, la importancia que le conceden a un conjunto de elementos para la práctica de la modalidad rural

  17. [Stain hybridization method with pRepHind probe for the diagnosis of Plasmodium falciparum].

    Science.gov (United States)

    Moleón Borodowsky, I

    1992-01-01

    A study was conducted on the parasitemia detection level and the specificity of the pRepHind DNA probe for diagnosing Plasmodium falciparum by the stain hybridization method. The parasitemia detection level was studied by using dilutions of a P. falciparum in vitro culture, adjusted by direct microscopic examination to 1; 0.1; 0.01; 0.001; 0.0001 and 0.00001% of parasited red cells. Specificity was increased by using DNA extractions from P. Yoelii, P. berghei and human leucocytes. The results showed that the method was able to detect 0.0001% of parasitemia starting from DNA extractions of 100 L infected red cells. The pRepHind probe only detected specifically DNA from P. falciparum. It is concluded that the method is suitable for being used in the diagnosis of infection due to P. falciparum.

  18. A república e o sonho The republic and its dream

    Directory of Open Access Journals (Sweden)

    Maria Tereza Chaves de Mello

    2011-06-01

    Full Text Available O desencanto com a República brasileira tem sido tema de reflexões desde os seus momentos iniciais. Buscando os conteúdos concretos dos sonhos republicanos na década de 1880 e na Primeira República vamos achar uma importante defasagem entre eles não exatamente em função do marco cronológico, mas do grupo social que, respectivamente, os acalentou.The disillusion with the Brazilian Republic since its first days has been a topic of academician reflexions. Examinating the concrete contents of the 1880`s and the First Republic republican dreams we will find a substantive difference between them. To be exact, this difference must not be related to the chronological demarcation but to the social group that embraced each of them.

  19. Optimization and head-to-head comparison of MISSR-PCR, ERIC-PCR, RAPD and 16S rRNA evolutionary clock for the genotyping of Vibrio cholerae isolated in China.

    Science.gov (United States)

    Mo, Q H; Wang, H B; Tan, H; An, S L; Feng, Z L; Wang, Q; Lin, J C; Yang, Z

    2015-01-01

    To establish a new genotyping method for Vibrio cholerae and compare it with other methods. In the current study, a modified inter simple sequence repeat-polymerase chain reaction (MISSR-PCR) system was developed via several rounds of optimisation. Comparison study was then conducted between MISSR-PCR and three other methods, including enterobacterial repetitive intergenic consensus sequences-based PCR (ERIC-PCR), randomly amplified polymorphic DNA (RAPD) and 16S rRNA evolutionary clock, for the detection and genetic tracing of Vibrio cholerae isolated from seafood in China. The results indicated that the MISSR-PCR system could generate the highest polymorphic fingerprinting map in a single round PCR and showed the best discriminatory ability for Vibrio cholerae genotyping by clearly separating toxigenic/nontoxigenic strains, local/foreign strains, and O1/O139/non-O1/non-O139 serogroup strains, comparing to ERIC-PCR, RAPD and 16S rRNA evolutionary clock. Moreover, the MISSR-PCR is superior to previously described traditional simple sequence repeat based PCR method on genotyping by more clearly separating different clusters. To the best of our knowledge, this is the first head-to-head comparison of four detection and genotyping methods for Vibrio cholerae The MISSR-PCR system established here could serve as a simple, quick, reliable and cost-effective tool for the genotyping and epidemiological study.

  20. Optimization and head-to-head comparison of MISSR-PCR, ERIC-PCR, RAPD and 16S rRNA evolutionary clock for the genotyping of Vibrio cholerae isolated in China

    Directory of Open Access Journals (Sweden)

    Q H Mo

    2015-01-01

    Full Text Available Purpose: To establish a new genotyping method for Vibrio cholerae and compare it with other methods. Materials and Methods: In the current study, a modified inter simple sequence repeat-polymerase chain reaction (MISSR-PCR system was developed via several rounds of optimisation. Comparison study was then conducted between MISSR-PCR and three other methods, including enterobacterial repetitive intergenic consensus sequences-based PCR (ERIC-PCR, randomly amplified polymorphic DNA (RAPD and 16S rRNA evolutionary clock, for the detection and genetic tracing of Vibrio cholerae isolated from seafood in China. Result: The results indicated that the MISSR-PCR system could generate the highest polymorphic fingerprinting map in a single round PCR and showed the best discriminatory ability for Vibrio cholerae genotyping by clearly separating toxigenic/nontoxigenic strains, local/foreign strains, and O1/O139/non-O1/non-O139 serogroup strains, comparing to ERIC-PCR, RAPD and 16S rRNA evolutionary clock. Moreover, the MISSR-PCR is superior to previously described traditional simple sequence repeat based PCR method on genotyping by more clearly separating different clusters. Conclusion: To the best of our knowledge, this is the first head-to-head comparison of four detection and genotyping methods for Vibrio cholerae The MISSR-PCR system established here could serve as a simple, quick, reliable and cost-effective tool for the genotyping and epidemiological study.

  1. Tratamiento tributario actual del comercio electrónico en la República Argentina

    OpenAIRE

    Martínez Giribone, María Silvana

    2010-01-01

    Tratamiento tributario actual del comercio electrónico en la República Argentina. PROBLEMA: ¿Constituye la legislación tributaria argentina aplicada a operaciones comerciales tradicionales un marco legal acorde para gravar operaciones de comercio electrónico? OBJETIVOS DE LA INVESTIGACION OBJETIVO GENERAL Analizar la aplicación de la legislación tributaria argentina para operaciones de comercio tradicionales en el comercio electrónico. OBJETIVOS ESPECIFICOS Ana...

  2. Vanguardismo y problemática del film alemán de la República de Weimar

    OpenAIRE

    Sánchez-Biosca, Vicente

    1985-01-01

    El presente artículo trata de examinar las aproximaciones historiográficas realizadas al cine producido en la República de Weimar, desde el llamado expresionismo hasta las corrientes realistas, pasando por el Kammerspielfilm.

  3. Reps võib kõrvale heita kümme miljonit maksnud õppekava / Dagne Hanschmidt

    Index Scriptorium Estoniae

    Hanschmidt, Dagne

    2005-01-01

    TÜ õppekavade arenduskeskus ja AS Bit koostavad mõlemad riikliku õppekava, lähtudes erinevatest põhimõttest. Haridus- ja teadusminister Mailis Reps ei ole avaldanud, kumba õppekava varianti ta eelistab

  4. A method mediated AAVS1 recombination with Rep mRNA and homologous arms

    Institute of Scientific and Technical Information of China (English)

    Qiantong Xiang; Linian Huang; Sijia Guo; Fang Chen; Xiaojun Zha; Bing Chen; Liangdan Sun; Haisheng Zhou; Depei Liu

    2012-01-01

    The adeno-associated virus (AAV) genome can be stably integrated into the AAVS1 region of human chromosome 19 (19q13.4-qter) with the assistance of Rep68/78 protein.In the current models of AAV integration in a locusspecific manner,the foreign genes were randomly inserted into the AAVS1 region,which contains several functional genes.As random integration in this region may lead to insertion mutations and disrupt normal gene expression or critical signaling pathways of the host cells,it is necessary to find a precise insertion site in the AAVS1 region.Homologous recombination is the most accurate and versatile mechanism for such site-specific integration.To investigate site-specific integration in the AAVS1 region,a targeted vector containing two homologous arms derived from AAVS1 and a reporter gene was transfected into HeLa cells with or without Rep68/78 mRNA.The results indicated that transient expression of Rep68/78 in HeLa cells improved integration of the gene of interest at the AAVS1 locus in a site-specific manner.Compared with locus-specific integration reported in previous studies,sitespecific integration may minimize the risk associated with random DNA integration in the AAVS1 region,which might be helpful for gene therapy.

  5. Design and Implementation of Reconfigurable Embedded Processor (REP for AUV using FPGA

    Directory of Open Access Journals (Sweden)

    Mohd. Rizal Arshad

    2012-09-01

    Full Text Available The superscalar pipeline is something that the REP architecture resembles. The features that are similar in both architectures include rename, decode, fetch as well as in-order front-ends parts as substituted by dispatch units. The physical register file, execute unit, dynamic scheduler and task queue are contained by the out of-order execution core. The architecture also contains the REP that beholds an integrated system of shared-memory. A number of experiments with realistic benchmarks and an FPGA environment are used to synthesize design. The experiments conducted yielded in results that prove that the new architecture of the REP leads to higher performance of applications because of decreased processing time. And the core reason for the deduction of processing time is the simplified complexity of the frequency and area of the architecture. The results show that the least error tolerance is 1m by particle filter. Also, the optimum iteration level is 20 to get enhanceable navigation data for AUV.

  6. Realizace 3D tiskárny typu RepRap

    OpenAIRE

    Sochor, Marek

    2015-01-01

    Cílem bakalářské práce bude sestavení a ověření funkčnosti 3D tiskárny typu RepRap. Tiskárna bude sestavena z běžně dostupných dílů a pro zachování myšlenky RepRap také z dílů tisknutých. Po ověření funkčnosti 3D tiskárny bude vytisknuta identická součást na dvou typech tiskáren (3D tiskárna typu RepRap vs. profesionální tiskárna uPrint) za účelem zhodnocení a celkové analýzy, jak po stránce finanční, tak po stránce fyzické. Práce bude ukončena zhodnocením použitelnosti a konkurenceschopnosti...

  7. Modulation of pPS10 Host Range by Plasmid-Encoded RepA Initiator Protein

    Science.gov (United States)

    Maestro, Beatriz; Sanz, Jesús M.; Díaz-Orejas, Ramón; Fernández-Tresguerres, Elena

    2003-01-01

    We report here the isolation and analysis of novel repA host range mutants of pPS10, a plasmid originally found in Pseudomonas savastanoi. Upon hydroxylamine treatment, five plasmid mutants were selected for their establishment in Escherichia coli at 37°C, a temperature at which the wild-type form cannot be established. The mutations were located in different functional regions of the plasmid RepA initiation protein, and the mutants differ in their stable maintenance, copy number, and ability to interact with sequences of the basic replicon. Four of them have broadened their host range, and one of them, unable to replicate in Pseudomonas, has therefore changed its host range. Moreover, the mutants also have increased their replication efficiency in strains other than E. coli such as Pseudomonas putida and Alcaligenes faecalis. None of these mutations drastically changed the structure or thermal stability of the wild-type RepA protein, but in all cases an enhanced interaction with host-encoded DnaA protein was detected by gel filtration chromatography. The effects of the mutations on the functionality of RepA protein are discussed in the framework of a three-dimensional model of the protein. We propose possible explanations for the host range effect of the different repA mutants, including the enhancement of limiting interactions of RepA with specific host replication factors such as DnaA. PMID:12562807

  8. The neurobiology of repetitive behavior : of mice…

    NARCIS (Netherlands)

    Langen, Marieke; Kas, Martien J H; Staal, Wouter G; van Engeland, Herman; Durston, Sarah

    2011-01-01

    Repetitive and stereotyped behavior is a prominent element of both animal and human behavior. Similar behavior is seen across species, in diverse neuropsychiatric disorders and in key phases of typical development. This raises the question whether these similar classes of behavior are caused by simi

  9. Large-scale detection of repetitions.

    Science.gov (United States)

    Smyth, W F

    2014-05-28

    Combinatorics on words began more than a century ago with a demonstration that an infinitely long string with no repetitions could be constructed on an alphabet of only three letters. Computing all the repetitions (such as ∙∙∙TTT ∙∙∙ or ∙∙∙ CGACGA ∙∙∙ ) in a given string x of length n is one of the oldest and most important problems of computational stringology, requiring time in the worst case. About a dozen years ago, it was discovered that repetitions can be computed as a by-product of the Θ(n)-time computation of all the maximal periodicities or runs in x. However, even though the computation is linear, it is also brute force: global data structures, such as the suffix array, the longest common prefix array and the Lempel-Ziv factorization, need to be computed in a preprocessing phase. Furthermore, all of this effort is required despite the fact that the expected number of runs in a string is generally a small fraction of the string length. In this paper, I explore the possibility that repetitions (perhaps also other regularities in strings) can be computed in a manner commensurate with the size of the output.

  10. Verbal Repetitions and Echolalia in Alzheimer's Discourse

    Science.gov (United States)

    Da Cruz, Fernanda Miranda

    2010-01-01

    This article reports on an investigation of echolalic repetition in Alzheimer's disease (AD). A qualitative analysis of data from spontaneous conversations with MHI, a woman with AD, is presented. The data come from the DALI Corpus, a corpus of spontaneous conversations involving subjects with AD. This study argues that echolalic effects can be…

  11. Neurobehavioural Correlates of Abnormal Repetitive Behaviour

    Directory of Open Access Journals (Sweden)

    R. A. Ford

    1991-01-01

    Full Text Available Conditions in which echolalia and echopraxia occur are reviewed, followed by an attempt to elicit possible mechanisms of these phenomena. A brief description of stereotypical and perseverative behaviour and obsessional phenomena is given. It is suggested that abnormal repetitive behaviour may occur partly as a result of central dopaminergic dysfunction.

  12. Reducing Repetitive Speech: Effects of Strategy Instruction.

    Science.gov (United States)

    Dipipi, Caroline M.; Jitendra, Asha K.; Miller, Judith A.

    2001-01-01

    This article describes an intervention with an 18-year-old young woman with mild mental retardation and a seizure disorder, which focused on her repetitive echolalic verbalizations. The intervention included time delay, differential reinforcement of other behaviors, and self-monitoring. Overall, the intervention was successful in facilitating…

  13. Verbal Repetitions and Echolalia in Alzheimer's Discourse

    Science.gov (United States)

    Da Cruz, Fernanda Miranda

    2010-01-01

    This article reports on an investigation of echolalic repetition in Alzheimer's disease (AD). A qualitative analysis of data from spontaneous conversations with MHI, a woman with AD, is presented. The data come from the DALI Corpus, a corpus of spontaneous conversations involving subjects with AD. This study argues that echolalic effects can be…

  14. Freedom of Speech: A Clear and Present Need to Teach. ERIC Report.

    Science.gov (United States)

    Boileau, Don M.

    1983-01-01

    Presents annotations of 21 documents in the ERIC system on the following subjects: (1) theory of freedom of speech; (2) theorists; (3) research on freedom of speech; (4) broadcasting and freedom of speech; and (5) international questions of freedom of speech. (PD)

  15. Finding Education and Training Technology: A Gap between ERIC and NTIS.

    Science.gov (United States)

    Bush, Clesson B.

    1993-01-01

    Shows civilian applications for Department of Defense education and training efforts and instructional technology. A search showing a gap between the ERIC and NTIS (National Technical Information Service) databases covering government documents is described. Suggestions for additional research and an overview of federal statutes which include…

  16. Racism in African Children's Literature: A Critique of Eric Campbell's "The Year of the Leopard Song."

    Science.gov (United States)

    Osa, Osayimwense

    Eric Campbell, an English teacher, spent most of his working life in New Guinea and in East Africa, where he lived in the shadow of Kilimanjaro. He now lives in England and writes about Africa. People could expect an objective, and perhaps, a dispassionate account or depiction of African children and adults--their individual lives and…

  17. Socioeconomic and Social Aspects of Rural Youth: A Selected Topics Bibliography of ERIC Documents.

    Science.gov (United States)

    ERIC Clearinghouse on Rural Education and Small Schools, Las Cruces, NM.

    Resource materials, research documents, and developments related to the socioeconomic and social aspects of rural youth both in the US and internationally are cited in this bibliography of ERIC documents (including such subjects as status projections, mental health, occupations, dropouts, ethnicity, abortion, delinquency, education, environmental…

  18. Michael Eric Dyson: A Scholar and a Hip-Hop Preacher.

    Science.gov (United States)

    Fletcher, Michael A.

    2000-01-01

    Introduces Michael Eric Dyson, an African American man who grew up in the ghetto and eventually received a PhD from Princeton University. Today, he is a professor at DePaul University, the author of a radically revisionist new biography of Martin Luther King, Jr., a lecturer and talk show guest, a Baptist preacher, and a self-styled hip-hop…

  19. Teaching about World War II: An ERIC/ChESS Sample.

    Science.gov (United States)

    Schlene, Vickie L.

    1991-01-01

    Presents nine documents from the ERIC database dealing with teaching about World War II. Includes articles addressing the lessons of Pearl Harbor, the Holocaust, the wartime internment of Japanese Americans, industry's response to the war, and the moral lessons of Nazism. (SG)

  20. The World War II Homefront: An ERIC/ChESS Sample.

    Science.gov (United States)

    Pinhey, Laura

    2002-01-01

    Provides citations with abstracts from the ERIC database focusing on the U.S. homefront during World War II. Includes background information and teaching materials on topics such as popular music from 1941-1945, propaganda directed towards women, and learning about Japanese American internment. (CMK)

  1. ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine

    Directory of Open Access Journals (Sweden)

    Luisa Zanolli Moreno

    2011-12-01

    Full Text Available Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %, followed by serovars 14 (14 %, 5 (12 %, 13 (8 % and 2 (2 %, whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86% were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil.

  2. Encore: A Selction of Articles from ERIC/ECE Newsletters (Jan. 1971-Dec. 1972).

    Science.gov (United States)

    ERIC Clearinghouse on Early Childhood Education, Champaign, IL.

    This compilation of articles from newsletters issued by the ERIC Clearinghouse on Early Childhood Education, (January 1971 - December 1972) includes a wide variety of topics such as: teachers' developmental stages, family day care in Pasadena, television violence, children's altruistic lying, and the Home Start program. Also included is a complete…

  3. Reflecting on Attention-Deficient Hyperactivity Disorder and Disablement in Education with Eric Fromm

    Science.gov (United States)

    Veck, Wayne

    2012-01-01

    Drawing upon the thought of Eric Fromm, this paper argues that efforts to understand and counter behaviour that causes difficulties in education should begin in a critical and reflective engagement with both the conditions of human freedom and the character of educational institutions and society as a whole. Fromm's critique of dominating ideas…

  4. The School District Management Audit. ERIC Digest Series Number EA31.

    Science.gov (United States)

    Klauke, Amy

    This ERIC Digest discusses the issue of the school district management audit--a comprehensive examination of an organization in order to assess efficient use of resources and program effectiveness. Several aspects of the management audit issue are covered in question-and-answer format: (1) What is a management audit? (2) What areas are covered by…

  5. Learning about the Human Genome. Part 2: Resources for Science Educators. ERIC Digest.

    Science.gov (United States)

    Haury, David L.

    This ERIC Digest identifies how the human genome project fits into the "National Science Education Standards" and lists Human Genome Project Web sites found on the World Wide Web. It is a resource companion to "Learning about the Human Genome. Part 1: Challenge to Science Educators" (Haury 2001). The Web resources and…

  6. ERIC Resource Management Workshops--Final Report on Workshops for Standing Order Customers.

    Science.gov (United States)

    Robbins, J. Nevin

    A series of workshops was conducted to enhance the information management capability of organizations maintaining complete files of ERIC (Educational Resources Information Center) materials, such as microfiche collections, indexes, and related materials. Invitations to attend meetings in their respective regions were extended to 677 organizations…

  7. ERIC Resource Management Workshops--Special Report on School System Standing Order Customers.

    Science.gov (United States)

    Robbins, J. Nevin

    This report summarizes descriptive information on school systems and school system-related organizations that maintain complete files of ERIC (Educational Resources Information Center) materials. The data reported were provided by participants in a series of regional workshops held throughout the United States on information management practices…

  8. The World War II Homefront: An ERIC/ChESS Sample.

    Science.gov (United States)

    Pinhey, Laura

    2002-01-01

    Provides citations with abstracts from the ERIC database focusing on the U.S. homefront during World War II. Includes background information and teaching materials on topics such as popular music from 1941-1945, propaganda directed towards women, and learning about Japanese American internment. (CMK)

  9. Access Education Literature with ERIC on CD-ROM: An Introductory Workshop.

    Science.gov (United States)

    Roberts, Deanna

    This report provides instructional materials for a voluntary introductory workshop in the use of the Educational Resources and Information Clearinghouse (ERIC) database on CD-ROM. The workshop instruction is based on Leslie J. Brigg's Theories of Instructional Design with instructional objectives based on Robert Gagne's Learning Taxonomy. Library…

  10. Automating the School Library Media Center. A Select ERIC Bibliography. Mini-Bib.

    Science.gov (United States)

    Preston, Nancy R., Comp.

    This 13-item annotated bibliography was compiled through a search of the Educational Resources Information Center (ERIC) database using the following descriptors: Library Automation, Bibliographic Databases, Bibliographic Utilities, Online Catalogs, Machine Readable Cataloging, and Integrated Library Systems. Among the issues addressed are the…

  11. Freedom of Speech: A Clear and Present Need to Teach. ERIC Report.

    Science.gov (United States)

    Boileau, Don M.

    1983-01-01

    Presents annotations of 21 documents in the ERIC system on the following subjects: (1) theory of freedom of speech; (2) theorists; (3) research on freedom of speech; (4) broadcasting and freedom of speech; and (5) international questions of freedom of speech. (PD)

  12. ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine.

    Science.gov (United States)

    Moreno, Luisa Zanolli; Castilla, Karina Salvagni; de Gobbi, Débora Dirani Sena; Coutinho, Tania Alen; Ferreira, Thais Sebastiana Porfida; Moreno, Andrea Micke

    2011-10-01

    Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil.

  13. Using Film To Teach History: An ERIC/ChESS Sample.

    Science.gov (United States)

    Pinhey, Laura

    2002-01-01

    Provides citations with abstracts from the ERIC database focusing on using film to teach history. Includes background information and teaching materials on topics such as using documentary films, the use of film and television to teach history, and teaching history using the film,"JFK," by Oliver Stone. (CMK)

  14. Translation initiation of the replication initiator repB gene of promiscuous plasmid pMV158 is led by an extended non-SD sequence.

    Science.gov (United States)

    López-Aguilar, Celeste; Ruiz-Masó, José A; Rubio-Lepe, Tania Samir; Sanz, Marta; del Solar, Gloria

    2013-07-01

    RepB is the pMV158-encoded protein that initiates rolling-circle replication of this promiscuous plasmid. Availability of RepB is rate-limiting for the plasmid replication process, and therefore the repB gene encoding the protein is subjected to strict control. Two trans-acting plasmid elements, CopG and the antisense RNAII, are involved in controlling the synthesis of the initiator at the transcriptional and translational level, respectively. In addition to this dual control of repB expression that senses and corrects fluctuations in plasmid copy number, proper availability of RepB also relies on the adequate functionality of the transcription and translation initiation regulatory signals. Translation of repB has been postulated to depend on an atypical ribosome binding site that precedes its start codon, although such a hypothesis has never been proved. To define sequences involved in translation of repB, several mutations in the translation initiation region of the repB mRNA have been characterized by using an Escherichia coli in vitro expression system wherein the synthesis of RepB was detected and quantified. We showed that translation of repB is not coupled to that of copG and depends only on its own initiation signals. The atypical ribosome binding site, as it was defined, is not involved in translation initiation. However, the sequence just upstream of the repB start codon, encompassing the proximal box of the atypical ribosome binding site and the four bases immediately downstream of it, is indeed important for efficient translation of repB. The high degree of conservation of this sequence among the rep genes of plasmids of the same pMV158 family supports its relevancy as a translation initiation signal in mRNAs without a recognizable Shine-Dalgarno sequence.

  15. EMSO ERIC - Ocean Consortium Facility for Europe and the World

    Science.gov (United States)

    Best, Mairi

    2017-04-01

    EMSO is forging ahead through the next challenge in Earth-Ocean Science: How to co-ordinate ocean data acquisition, analysis and response across provincial, national, regional, and global scales. EMSO provides power, communications, sensors, and data infrastructure for continuous, high resolution, real-time, interactive ocean observations across a truly multi- and interdisciplinary range of research areas including biology, geology, chemistry, physics, engineering, and computer science; from polar to tropical environments, through the water column down to the abyss. 11 deep sea and 4 shallow nodes span from Arctic through the Atlantic and Mediterranean, to the Black Sea. The EMSO Preparatory Phase (FP7) project led to the Interim phase (involving 13 countries) of forming the legal entity: the EMSO European Research Infrastructure Consortium (EMSO-ERIC)-officially created by the EC in 2016. The open user community, originally developed through ESONET (European Seafloor Observatory NETwork), follows on scientific community planning contributions of the ESONET-NoE (FP6) project. Further progress made through the FixO3 project (FP7) also contributes to this shared infrastructure. Coordination among nodes is being strengthened through the EMSOdev project (H2020) which is producing the EMSO Generic Instrument Module (EGIM) - standardised observations of temperature, pressure, salinity, dissolved oxygen, turbidity, chlorophyll fluorescence, currents, passive acoustics, pH, pCO2, and nutrients. Early installations are now being upgraded; in October 2015 EMSO-France deployed a second cable and junction box serving the Ligurian Sea Node in order to monitor slope stability offshore Nice; in 2016 the EMSO Azores Node receives a major upgrade that will double its observing capacity; for EMSO-Italia the Capo Passero site is being installed and the Catania site is being upgraded. EMSOLINK will continue the expansion work. EMSO is a key player in international coordination

  16. 重复序列PCR与多位点分型技术在热带假丝酵母菌基因分型中的比较%Comparative study on genotyping of Candida tropicalis by repetitive sequence-based PCR and multilocus sequence typing

    Institute of Scientific and Technical Information of China (English)

    江岑; 董丹凤; 俞焙秦; 彭奕冰

    2012-01-01

    目的 分析比较重复序列聚合酶链反应(REP-PCR)与多位点分型技术(MLST)在热带假丝酵母菌基因分型中的应用.方法 收集来自5个地区6家医院的147株热带假丝酵母菌,分别以Ca-21、Ca-22、Com-21两两组合为引物,选用最合适的引物对进行 REP-PCR后通过电泳获得REP-PCR型.在不同型别中各挑选3株采用MLST法扩增热带假丝酵母菌的6个管家基因,扩增片段测序后与数据库比对得到相应的序列型(sequence type,ST).结果 REP-PCR以Com21-Com21为引物对分型效果最好,REP-PCR与MLST分型结果一致.147株热带假丝酵母菌产生A~H共 8种REP-PCR型,分别对应MLST的ST146、新型1、ST136、ST127、ST177、ST169、新型2和ST117.结论 REP-PCR与MLST在热带假丝酵母菌的基因分型中分辨率相同,而REP-PCR更为方便迅速,可作为实验室大量菌株分型的首选方法.%Objective To compare repetitive sequence-based polymerase chain reaction ( REP-PCR ) and multilocus sequence typing ( MLST)in genotyping of Candida tropicalis. Methods REP-PCR was performed on 147 clinical isolates of Candida tropicalis collected from 6 hospitals of 5 provinces. Primer Ca-21, Ca-22 and Com-21 were used pairly to find the most suitable pair. Three isolates of Candida tropicalis from different REP-PCR types were tested by MLST. Six loci in housekeeping genes were sequenced after amplification, which were compared with the MLST database to obtain sequence type ( ST ). Results Eight REP-PCR types were found in 147 isolates of Candida tropicalis with primer Com21-Com21, which had the best genotyping effect. Type A-H were corresponding with ST146,NEW1, ST136,ST127,ST177,ST169,NEW2 and ST117 by MLST respectively. Conclusions REP-PCR offers a simple and rapid method for molecular typing, which has a similar discriminatory power with MLST. Therefore, REP-PCR can be the first choice in laboratory, especially for a large number of isolates.

  17. Impact of capsid conformation and Rep-capsid interactions on adeno-associated virus type 2 genome packaging.

    Science.gov (United States)

    Bleker, Svenja; Pawlita, Michael; Kleinschmidt, Jürgen A

    2006-01-01

    Single-stranded genomes of adeno-associated virus (AAV) are packaged into preformed capsids. It has been proposed that packaging is initiated by interaction of genome-bound Rep proteins to the capsid, thereby targeting the genome to the portal of encapsidation. Here we describe a panel of mutants with amino acid exchanges in the pores at the fivefold axes of symmetry on AAV2 capsids with reduced packaging and reduced Rep-capsid interaction. Mutation of two threonines at the rim of the fivefold pore nearly completely abolished Rep-capsid interaction and packaging. This suggests a Rep-binding site at the highly conserved amino acids at or close to the pores formed by the capsid protein pentamers. A different mutant (P. Wu, W. Xiao, T. Conlon, J. Hughes, M. Agbandje-McKenna, T. Ferkol, T. Flotte, and N. Muzyczka, J. Virol. 74:8635-8647, 2000) with an amino acid exchange at the interface of capsid protein pentamers led to a complete block of DNA encapsidation. Analysis of the capsid conformation of this mutant revealed that the pores at the fivefold axes were occupied by VP1/VP2 N termini, thereby preventing DNA introduction into the capsid. Nevertheless, the corresponding capsids had more Rep proteins bound than wild-type AAV, showing that correct Rep interaction with the capsid depends on a defined capsid conformation. Both mutant types together support the conclusion that the pores at the fivefold symmetry axes are involved in genome packaging and that capsid conformation-dependent Rep-capsid interactions play an essential role in the packaging process.

  18. DNA Fingerprinting of Intestinal Flora in Rats with Slow Transit Constipation%慢传输便秘大鼠肠道菌群的ERIC-PCR指纹图谱分析

    Institute of Scientific and Technical Information of China (English)

    侯翔宇; 王维林; 李勇

    2013-01-01

    Objective To analyze the difference of intestinal flora between experimental rats with slow transit constipation (STC) and rats in control group by enter bacterial repetitive intergenic consensus (ERIC)-PCR. Methods Of 30 Wistar rats, 15 were randomized to the control group and the other 15 were randomized to model group. We induced STC rats with loperamide by gavage. Fecal pellet numbers,fecal weights were determined. Record the weighing of food and water every 24 h. To evaluate gastrointestinal (GI) transit time, two groups of 30 rats were catheters passed into their stomach to allow for administration of a charcoal meal solution via gavage. DNA fingerprinting was set up by using ERIC-PCR technology, and the total difference of their intestinal flora was analyzed. Results Following a 24-day experiment with lopramide,the total fecal mass produced by model group was less than that produced by control rats (4.80±1.48)g vs (9.20±1.30)g,P 0.05. After loperamide administration,we observed an increase in the mean GI transit time as compared to the control period (691.60±220.03)min vs (437.20±43.89)min,P < 0.05. The ERIC-PCR electrophoresis showed that there were individual variations of the gut microbiota in rats,while there were common strap in two groups. The diversity of intestinal bacterial composition remarkably increased at the 24th day after giving loperamide in model group. Conclusion Rats model with slow transit constipation were established successfully by feeding loperamide. The diversity of intestinal bacterial composition remarkably increased at the 24th day after giving loperamide.%目的 应用细菌基因共有重复序列指纹图谱技术,探讨慢传输便秘肠道菌群的变化.方法 选取Wistar大鼠30只,随机分为对照组15只、造模组15只,造模组给予易蒙停灌胃.收集每24 h排出粪便,计数排便粒数,称量粪便质量,及24 h进食量及进水量,通过碳末灌胃测定肠道传输时间.留取造模组与对照组

  19. The EMSO-ERIC Pan-European Consortium: Data Benefits and Lessons Learned as the Legal Entity Forms

    NARCIS (Netherlands)

    Best, M.M.R.; Favali, P.; Beranzoli, L.; Blandin, J.; Cagatay, N.M.; Cannat, M.; Dañobeitia, J.J.; Delory, E.; de Miranda, J.M.A.; Del Rio Fernandez, J.; De Stigter , H.; Gillooly, M.; Grant, F.; Hall, P.O.J.; Hartman, S.E.; Hernandez-Brito, J.; Lanteri, N.; Mienert, J.; Oaie, G.; Piera, J.; Radulescu, V.; Rolin, J.-F.; Ruhl, H.A.; Waldmann, C.

    2016-01-01

    The European Multidisciplinary Seafloor and water-column Observatory (EMSO) European Research Infrastructure Consortium (ERIC) provides power, communications, sensors, and data infrastructure for continuous, high-resolution, (near-)real-time, interactive ocean observations across a multidisciplinary

  20. "Innovation and Intellectual Property Policies in European Research Infrastructure Consortia - PART I: The Case of the European Spallation Source ERIC"

    DEFF Research Database (Denmark)

    Yu, Helen; Wested, Jakob; Minssen, Timo

    2017-01-01

    of the problems society is facing today. To facilitate the creation and operation of such RIs, the EU adopted legal frameworks for European Research Infrastructure Consortia (ERIC). On August 31, 2015, the European Spallation Source (ESS) was established as an ERIC. Under the ERIC Regulations and ESS Statutes......Research and innovation are key pillars of the EU’s strategy to create sustainable growth and prosperity in Europe. Research infrastructures (RIs) are central instruments to implement this strategy. They bring together a wide diversity of expertise and interests to look for solutions to many......, the European Spallation Source ERIC is required to adopt various policy documents relating to the operation and management of the facility. These cover a wide variety of issues such as user access, public procurement, intellectual property rights (IPR), data management, and dissemination. One of the main goals...

  1. Transcriptomics of tomato plants infected with TYLCSV or expressing the central TYLCSV Rep protein domain uncover changes impacting pathogen response and senescence.

    Science.gov (United States)

    Lucioli, Alessandra; Perla, Carlo; Berardi, Alessandra; Gatti, Francesca; Spanò, Laura; Tavazza, Mario

    2016-06-01

    To establish a successful infection viruses need to overcome plant innate immune responses and redirect host gene expression for their multiplication and diffusion. Tomato yellow leaf curl Sardinia virus (TYLCSV) is a geminivirus, which causes significant economic losses in tomato. The multifunctional replication associated geminivirus protein (Rep) has an important role during viral infection. In particular, the Rep central domain spanning from aa 120 to 180 is known to interact with viral and host factors. In this study, we used long serial analysis of gene expression to analyse the transcriptional profiles of transgenic tomato plants expressing the first 210 amino acids of TYLCSV Rep (Rep210) and TYLCSV-infected wild-type tomato plants (Wt-Ty). Also, we compared these profiles with those of transgenic Rep130 tomatoes. Comparison of Wt-Ty and Rep210 libraries with the wild-type one identified 118 and 203 differentially expressed genes (DEGs), respectively. Importantly, 55% of Wt-Ty DEGs were in common with Rep210, and no ones showed opposite expression. Conversely, a negligible overlap was found between Rep130 DEGs and Wt-Ty and Rep210 ones. TYLCSV- and Rep210-repressed genes, but not induced ones, overlapped with the leaf senescence process. Interestingly, TYLCSV upregulates expression of genes involved in the negative regulation of programmed cell death (PCD), several of which were also regulated by the abscisic acid. Rep210 upregulated genes related to defence response, immune system processes and negative regulation of PCD. Collectively, our results support a model in which the Rep central domain has a pivotal role in redirecting host plant gene expression.

  2. Robust Repetitive Controller for Fast AFM Imaging

    CERN Document Server

    Necipoglu, Serkan; Has, Yunus; Guvenc, Levent; Basdogan, Cagatay

    2012-01-01

    Currently, Atomic Force Microscopy (AFM) is the most preferred Scanning Probe Microscopy (SPM) method due to its numerous advantages. However, increasing the scanning speed and reducing the interaction forces between the probe's tip and the sample surface are still the two main challenges in AFM. To meet these challenges, we take advantage of the fact that the lateral movements performed during an AFM scan is a repetitive motion and propose a Repetitive Controller (RC) for the z-axis movements of the piezo-scanner. The RC utilizes the profile of the previous scan line while scanning the current line to achieve a better scan performance. The results of the scanning experiments performed with our AFM set-up show that the proposed RC significantly outperforms a conventional PI controller that is typically used for the same task. The scan error and the average tapping forces are reduced by 66% and 58%, respectively when the scan speed is increased by 7-fold.

  3. High-repetition rate relativistic electron beam generation from intense laser solid interactions

    Science.gov (United States)

    Batson, Thomas; Nees, John; Hou, Bixue; Thomas, A. G. R.; Krushelnick, Karl

    2015-05-01

    Relativistic electron beams have applications spanning materials science, medicine, and home- land security. Recent advances in short pulse laser technology have enabled the production of very high focused intensities at kHz rep rates. Consequently this has led to the generation of high ux sources of relativistic electrons- which is a necessary characteristic of these laser plasma sources for any potential application. In our experiments, through the generation of a plasma with the lambda cubed laser system at the University of Michigan (a 5 × 1018W=cm2, 500 Hz, Ti:Sapphire laser), we have measured electrons ejected from the surface of fused silica nd Cu targets having energies in excess of an MeV. The spectrum of these electrons was measured with respect to incident laser angle, prepulse timing, and focusing conditions. While taken at a high repetition rate, the pulse energy of the lambda cubed system was consistently on the order of 10 mJ. In order to predict scaling of the electron energy with laser pulse energy, simulations are underway which compare the spectrum generated with the lambda cubed system to the predicted spectrum generated on the petawatt scale HERCULES laser system at the University of Michigan.

  4. A repetitive elements perspective in Polycomb epigenetics.

    Directory of Open Access Journals (Sweden)

    Valentina eCasa

    2012-10-01

    Full Text Available Repetitive elements comprise over two-thirds of the human genome. For a long time, these elements have received little attention since they were considered non functional. On the contrary, recent evidence indicates that they play central roles in genome integrity, gene expression and disease. Indeed, repeats display meiotic instability associated with disease and are located within common fragile sites, which are hotspots of chromosome rearrangements in tumors. Moreover, a variety of diseases have been associated with aberrant transcription of repetitive elements. Overall this indicates that appropriate regulation of repetitive elements’ activity is fundamental.Polycomb group (PcG proteins are epigenetic regulators that are essential for the normal development of multicellular organisms. Mammalian PcG proteins are involved in fundamental processes, such as cellular memory, cell proliferation, genomic imprinting, X-inactivation, and cancer development. PcG proteins can convey their activity through long-distance interactions also on different chromosomes. This indicates that the 3D organization of PcG proteins contributes significantly to their function. However, it is still unclear how these complex mechanisms are orchestrated and which role PcG proteins play in the multi-level organization of gene regulation. Intriguingly, the greatest proportion of Polycomb-mediated chromatin modifications is located in genomic repeats and it has been suggested that they could provide a binding platform for Polycomb proteins.Here, these lines of evidence are woven together to discuss how repetitive elements could contribute to chromatin organization in the 3D nuclear space.

  5. Emotional arousal enhances word repetition priming

    OpenAIRE

    Thomas, Laura A.; LaBar, Kevin S.

    2005-01-01

    Three experiments were conducted to determine if emotional content increases repetition priming magnitude. In the study phase of Experiment 1, participants rated high-arousing negative (taboo) words and neutral words for concreteness. In the test phase, they made lexical decision judgements for the studied words intermixed with novel words (half taboo, half neutral) and pseudowords. In Experiment 2, low-arousing negative (LAN) words were substituted for the taboo words, and in Experiment 3 al...

  6. The Rhythms of Echo. Variations on Repetition

    Directory of Open Access Journals (Sweden)

    Rosa María Aradra Sánchez

    2015-04-01

    Full Text Available This paper presents a study on the echo as metric and rhetorical procedure. It makes a brief tour through some of the poetic manifestations of echo in the Spanish literary tradition, and a brief tour through the attention that metric theory has paid to this phenomenon. Then it stops at the possibilities that rhetoric offers for its analysis from the generic approach of the discursive repetition phenomena.

  7. Elecciones consulares en la República romana: control y manipulación

    OpenAIRE

    Muñiz Coello, Joaquín

    2013-01-01

    Tratamos en este artículo sobre la naturaleza de la autoridad del magistrado que preside los comicios electorales en la República romana, con relación a la liturgia de las diversas fases de los procesos y los actos previstos en los mismos. Consideramos relevante tratar de explicar las circuntancias de poder e influencia que caracterizaba al oficio, y la pugna que se desataba entre las factiones del senado, bien para colocar a sus candidatos, bien para influir en ...

  8. La teoría de la justicia en La República

    Directory of Open Access Journals (Sweden)

    David Sobrevilla

    1994-06-01

    Full Text Available En este artículo se presenta la teoría platónica de la justicia a nivel colectivoe individual en La República, siguiéndose los lineamientos del artículode G. Vlastos, "Justice and Happiness in theRepublic" (1971 . Pesea que se haya objetado a esta teoría de diversas maneras, ella aparece como habiendo iniciado la tradición utópica occidental de reflexión sobre elEstado justo.

  9. La democracia parlamentaria en la República de Weimar: entre el mito y la realidad

    OpenAIRE

    1998-01-01

    El artículo examina las principales teorías sobre el fracaso de la República de Weimar y el ascenso del nacional socialismo y llama la atención sobre la necesidad de estudiar en sí el período de Weimar para que, al margen de mitos de todo tipo, revele las deficiencias ideológicas y culturales de la primera experiencia democrática alemana. This article studies the main theories in relation to the failure of the Weimar Republic and the rise of the Nationalsocialist Dictatorship. Thus it call...

  10. Carreiras políticas e nepotismo na Terceira República francesa (1871-1940)

    OpenAIRE

    Niess,Alexandre

    2012-01-01

    Ainda que possa parecer improvável em um regime baseado no sufrágio universal masculino, as conexões familiares e o nepotismo cumpriram um papel extremamente importante na democracia representativa na Terceira República Francesa (1870-1940). Na verdade, essas práticas continuam importantes em algumas partes da esfera política até hoje. Este artigo examina detalhadamente as conexões e as práticas políticas no departamento de Marne, um espaço político onde vários tipos diferentes de conexões po...

  11. Lightweight Autonomous Underwater Vehicles (AUVs) performing coastal survey operations in REP 10A

    Science.gov (United States)

    Incze, Michael L.

    2011-11-01

    Lightweight Autonomous Underwater Vehicles (AUVs) were developed for Naval Special Warfare (NSW) Group 4 search and survey missions from a commercial AUV baseline (Iver 2) through integration of commercial off-the-shelf (COTS) hardware components, and through software development for enhanced on-board Command and Control functions. The development period was 1 year under a project sponsored by the Office of Naval Research TechSolutions Program Office. Hardware integration was completed by the commercial AUV vendor, OceanServer Technology, Inc., and software development was conducted by the Naval Undersea Warfare Center, Naval Oceanographic Office, and U MASS Dartmouth, with support from hardware and software application providers (YSI, Inc., Imagenex Technology Corp., and CARIS). At the conclusion of the integration and development period, an at-sea performance evaluation was scheduled for the Lightweight NSW AUVs with NSWG-4 personnel. The venue for this evaluation was the NATO exercise Recognized Environmental Picture 10A (REP 10A), hosted by Marinha Portuguesa, and coordinated by the Faculdade de Engenharia-Universidade do Porto. REP 10A offered an opportunity to evaluate the performance of the new AUVs and to explore the Concept of Operations (CONOPS) for employing them in military survey operations in shallow coastal waters. Shore- and ship-launched scenarios with launch/recovery by a single operator in a one-to-many coordinated survey, on-scene data product generation and visualization, data push to Reach Back Cells for product integration and enhancement, and survey optimization to streamline survey effort and timelines were included in the CONOPS review. Opportunities to explore employment of hybrid AUV fleets in Combined Force scenarios were also utilized. The Naval Undersea Warfare Center, Marinha Portuguesa, the Faculdade de Engenharia-Universidade do Porto, and OceanServer Technology, Inc., were the primary participants bringing in-water resources to

  12. El separatismo en la República Popular China : El caso de los Uigures en Xinjiang

    OpenAIRE

    Sarli, Corina; Schiaffi, Belén

    2010-01-01

    Con la intención de profundizar el estudio de diferentes aspectos de las relaciones internacionales, se considera relevante analizar aquellos movimientos separatistas que trascienden los conflictos propios de un Estado. En este sentido, es nuestra intención centrarnos en uno de los conflictos internos mas importantes que esta atravesando el Estado que hoy es considerado la segunda economía mundial, la República Popular China (RPCh). Así, el objetivo de este trabajo es estudiar el conflicto...

  13. Interview with eric R. Kandel: from memory, free will, and the problem with Freud to fortunate decisions.

    Science.gov (United States)

    Kandel, Eric R

    2008-04-24

    Eric R. Kandel shared the Nobel Prize for Physiology or Medicine with Arvid Carlsson and Paul Greengard in 2000 for their discoveries concerning signal transduction in the nervous system. Eric Kandel was rewarded for his discoveries of molecular mechanisms underlying learning and memory. He is a Howard Hughes Medical Institute Senior Investigator at Columbia Universities Center for Neurobiology and Behavior. In this interview given at Hertie Foundation's Neuroforum 2008 on April 18, 2008 in Frankfurt, Germany, Nobel Prize Laureate Eric R. Kandel takes us on an enlighting journey ranging from memory, free will, the problem with Freud , to scientific challenges and the rise of European science. Starting with short- and long-term memory basics, underlying molecular mechanisms, and events affecting the formation of long-term memory, Eric Kandel then shares his thoughts on the issue of free will and makes a strong case for its existence. In addition to his outstanding scientific career, Eric Kandel developed an interest in business and today serves as scientific advisor at Memory Pharmaceuticals. Eric Kandel then talks about the use and abuse of drugs in children and the merging of scientific disciplines. In one of the most intriguing parts of the interview, Kandel shares his thoughts on Freud, Freud's mistakes, and the unique situation that the generations following Freud did not develop Freud's work further. The interview then turns to more scientific aspects and Eric Kandel talks about his confidence and courage during the initial phases of his scientific career, studying learning and memory, and employing a strictly reductionistic approach, best characterized by his simple model organism Aplysia. Eric Kandel then describes one of the most challenging phases in his scientific career when adult neurogenesis was discovered in the hippocampus, a structure so integral to Kandel's work. The final parts of the interview cover today's absence of epic battles and

  14. Repetitive behaviour in autism: Imaging pathways and trajectories

    NARCIS (Netherlands)

    Langen, M.J.G.

    2009-01-01

    Repetitive behaviour in autism: Imaging pathways and trajectories Repetitive and rigid behaviour is one of the core symptoms of autism, a severe and lifelong child psychiatric disorder. Although repetitive behaviour symptoms often form a significant impairment for affected individuals, systematic st

  15. Neural Correlates of Restricted, Repetitive Behaviors in Autism Spectrum Disorders

    Science.gov (United States)

    2014-12-01

    Restrictive Repetitive Behaviors in Autism Spectrum Disorder . Authors: T.Q.Nguyen, B...Manoach. Functional Connectivity of the Dorsal Anterior Cingulate Cortex Predicts Restrictive Repetitive Behaviors in Autism Spectrum Disorder We...Introduction: Although restricted , repetitive behaviors (RRBs) are a highly disabling core feature of Autism Spectrum Disorders (ASDs), they

  16. Lingual Kinematics during Rapid Syllable Repetition in Parkinson's Disease

    Science.gov (United States)

    Wong, Min Ney; Murdoch, Bruce E.; Whelan, Brooke-Mai

    2012-01-01

    Background: Rapid syllable repetition tasks are commonly used in the assessment of motor speech disorders. However, little is known about the articulatory kinematics during rapid syllable repetition in individuals with Parkinson's disease (PD). Aims: To investigate and compare lingual kinematics during rapid syllable repetition in dysarthric…

  17. Repetitive Elements in Mycoplasma hyopneumoniae Transcriptional Regulation

    Science.gov (United States)

    Cattani, Amanda Malvessi; Siqueira, Franciele Maboni; Guedes, Rafael Lucas Muniz; Schrank, Irene Silveira

    2016-01-01

    Transcriptional regulation, a multiple-step process, is still poorly understood in the important pig pathogen Mycoplasma hyopneumoniae. Basic motifs like promoters and terminators have already been described, but no other cis-regulatory elements have been found. DNA repeat sequences have been shown to be an interesting potential source of cis-regulatory elements. In this work, a genome-wide search for tandem and palindromic repetitive elements was performed in the intergenic regions of all coding sequences from M. hyopneumoniae strain 7448. Computational analysis demonstrated the presence of 144 tandem repeats and 1,171 palindromic elements. The DNA repeat sequences were distributed within the 5’ upstream regions of 86% of transcriptional units of M. hyopneumoniae strain 7448. Comparative analysis between distinct repetitive sequences found in related mycoplasma genomes demonstrated different percentages of conservation among pathogenic and nonpathogenic strains. qPCR assays revealed differential expression among genes showing variable numbers of repetitive elements. In addition, repeats found in 206 genes already described to be differentially regulated under different culture conditions of M. hyopneumoniae strain 232 showed almost 80% conservation in relation to M. hyopneumoniae strain 7448 repeats. Altogether, these findings suggest a potential regulatory role of tandem and palindromic DNA repeats in the M. hyopneumoniae transcriptional profile. PMID:28005945

  18. Modeling repetitive motions using structured light.

    Science.gov (United States)

    Xu, Yi; Aliaga, Daniel G

    2010-01-01

    Obtaining models of dynamic 3D objects is an important part of content generation for computer graphics. Numerous methods have been extended from static scenarios to model dynamic scenes. If the states or poses of the dynamic object repeat often during a sequence (but not necessarily periodically), we call such a repetitive motion. There are many objects, such as toys, machines, and humans, undergoing repetitive motions. Our key observation is that when a motion-state repeats, we can sample the scene under the same motion state again but using a different set of parameters; thus, providing more information of each motion state. This enables robustly acquiring dense 3D information difficult for objects with repetitive motions using only simple hardware. After the motion sequence, we group temporally disjoint observations of the same motion state together and produce a smooth space-time reconstruction of the scene. Effectively, the dynamic scene modeling problem is converted to a series of static scene reconstructions, which are easier to tackle. The varying sampling parameters can be, for example, structured-light patterns, illumination directions, and viewpoints resulting in different modeling techniques. Based on this observation, we present an image-based motion-state framework and demonstrate our paradigm using either a synchronized or an unsynchronized structured-light acquisition method.

  19. Repetitive element hypermethylation in multiple sclerosis patients.

    Science.gov (United States)

    Neven, K Y; Piola, M; Angelici, L; Cortini, F; Fenoglio, C; Galimberti, D; Pesatori, A C; Scarpini, E; Bollati, V

    2016-06-18

    Multiple sclerosis (MS) is a complex disorder of the central nervous system whose cause is currently unknown. Evidence is increasing that DNA methylation alterations could be involved in inflammatory and neurodegenerative diseases and could contribute to MS pathogenesis. Repetitive elements Alu, LINE-1 and SAT-α, are widely known as estimators of global DNA methylation. We investigated Alu, LINE-1 and SAT-α methylation levels to evaluate their difference in a case-control setup and their role as a marker of disability. We obtained blood samples from 51 MS patients and 137 healthy volunteers matched by gender, age and smoking. Methylation was assessed using bisulfite-PCR-pyrosequencing. For all participants, medical history, physical and neurological examinations and screening laboratory tests were collected. All repetitive elements were hypermethylated in MS patients compared to healthy controls. A lower Expanded Disability Status Scale (EDSS) score was associated with a lower levels of LINE-1 methylation for 'EDSS = 1.0' and '1.5 ≤ EDSS ≤ 2.5' compared to an EDSS higher than 3, while Alu was associated with a higher level of methylation in these groups: 'EDSS = 1.0' and '1.5 ≤ EDSS ≤ 2.5'. MS patients exhibit an hypermethylation in repetitive elements compared to healthy controls. Alu and LINE-1 were associated with degree of EDSS score. Forthcoming studies focusing on epigenetics and the multifactorial pathogenetic mechanism of MS could elucidate these links further.

  20. FRB repetition and non-Poissonian statistics

    CERN Document Server

    Connor, Liam; Oppermann, Niels

    2016-01-01

    We discuss some of the claims that have been made regarding the statistics of fast radio bursts (FRBs). In an earlier paper \\citep{2015arXiv150505535C} we conjectured that flicker noise associated with FRB repetition could show up in non-cataclysmic neutron star emission models, like supergiant pulses. We show how the current limits of repetition would be significantly weakened if their repeat rate really were non-Poissonian and had a pink or red spectrum. Repetition and its statistics have implications for observing strategy, generally favouring shallow wide-field surveys, since in the non-repeating scenario survey depth is unimportant. We also discuss the statistics of the apparent latitudinal dependence of FRBs, and offer a simple method for calculating the significance of this effect. We provide a generalized Bayesian framework for addressing this problem, which allows for direct model comparison. It is shown how the evidence for a steep latitudinal gradient of the FRB rate is less strong than initially s...

  1. Isotopic separation through ion cyclotron-resonance: results from the ERIC experiment; La separation isotopique par resonance cyclotron ionique: resultats obtenus avec l`experience ERIC

    Energy Technology Data Exchange (ETDEWEB)

    Compant la Fontaine, A.; Louvet, P. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. des Procedes d`Enrichissement

    1994-12-31

    Stable isotope separation by the means of ion cyclotron-resonance is studied at CEA since 1981. Results from the ERIC experiments are of two types: parameter measurements which help characterizing the plasma and optimizing the process, and isotopic separation results. For example, the selective feature of isotope heating was verified in the cases of zinc and calcium using an electrostatic analyzer. The separation factors of various elements (calcium, zinc, barium, chromium...) are depending on the mass relative difference of the isotopes to be separated, difference which must be large compared to the magnetic field inhomogeneities and the Doppler broadening. 2 figs., 1 tab., 3 refs.

  2. Realidades de la independencia del Banco de la República Realities of the independence of the Banco de la República

    Directory of Open Access Journals (Sweden)

    Kalmanovitz Salomón

    1997-12-01

    Full Text Available La autonomía del banco central Colombiano es parte del proceso político que buscaba reducir el exagerado centralismo colombiano. Sin embargo el poder central no fue limitado en grado suficiente por la Constitución del 1991, como muestra el hecho de que el Ministro de Hacienda es el Presidente de la Junta Directiva del Banco de la República. Esto no sucede en ningun banco independiente del mundo por que los objetivos del gobierno son distintos a los del Banco Central y el Ministerio de Finanzas podría desviar al Banco de sus objetivos antiinflacionarios fundamentales. En Colombia, el gobierno no sólo participa en la Junta para acomodar sus múltiples objetivos sino también hace opocisión pública a la política monetaria y así la desacredita. El articulo plantea que el principio de no reelección hace que el horizonte de tiempo sea muy corto para que el gobierno coseche los frutos de una política de estabilización. El gobierno tiende a gastar en exceso para mejorar su imagen pública y el desajuste lo tiene que enfrentar el gobierno siguiente. De modo que la coordinación entre el Banco Central y el gobierno se torna muy conflictiva.The autonomy of the Central Bank of Colombia is part of a political process which seeks to reduce Colombia' s exaggerated centralismo However, central power was not limited to a sufficient degree by the Constitution of 1991, as demonstrated by the fact that the Minister of Finance is the President of the Board of Directors of the Banco de la República. This does not happen in any independent bank in the world, because the objectives of the government are different from those of the central bank, and the Minister of Finance could divert the Bank away from its fundamental anti-inflationary objectives. This article proposes that the principle of no reelection makes the time horizon very short for the government to reap the fruits of a stabilization policy. The government tends to spend in excess in order to

  3. Estado actual de la odontopediatría en la República Mexicana

    Directory of Open Access Journals (Sweden)

    Enrique E .-Huitzil-Muñóz

    2012-02-01

    Full Text Available La odontología pediátrica satisface un propósito muy especial con respecto de las especialidades odontológicas orientada a la prevención y curación de las enfermedades del sistema estomatognático durante la infancia y considerada como una especialidad muy completa y relativamente nueva se realiza una revisión acerca de la historia de la odontología pedíatrica en el mundo y en especial en México, recopilando información importante acerca de las instituciones universitarias que cuentan en su curricula con la especialidad, certificación y zonas de ubicación en la República Mexicana. Conclusiones: De acuerdo a las regiones de la República Mexicana en las que se encuentran ubicados los diversos posgrados, se concluye que de un total de 30 universidades que cuentan con la especialidad o maestría en odontopediatría, existen 7 en la denominada región centro y golfo y 6 en la región México D.F., estos datos concuerdan con el elevado número de habitantes que habitan esas regiones y que necesitan atención odontopediátrica cubriendo así la alta demanda del servicio por parte de los odontólogos posgraduados egresados.

  4. Averróis e a República de Platão

    Directory of Open Access Journals (Sweden)

    Pereira, Rosalie Helena de Souza

    2007-01-01

    Full Text Available Sobre República é o único comentário de uma obra de Platão deixado por Averróis, o comentador por antonomásia, diga-se de Aristóteles. Embora se tenha perdido o original árabe, a teles. Embora se tenha perdido o original árabe, a posteridade recebeu uma versão hebraíca e duas latinas. O comentário (ou paráfrase a República de Platão pode ser considerado uma obra original - divida em três livros - já que apenas um terço dela corresponde ao texto platônico. Além de apresentar conceitos aristotélicos retirados da Ética Nicomaquéia e de obras políticas de Al-Farabi, Averróis apresenta reflexões próprias e faz críticas à sociedade em que vive

  5. Four types of (super)conformal mechanics: D-module reps and invariant actions

    CERN Document Server

    Holanda, N L

    2014-01-01

    (Super)conformal mechanics in one dimension is induced by parabolic or hyperbolic/trigonometric transformations, either homogeneous (for a scaling dimension $\\lambda$) or inhomogeneous (at $\\lambda=0$, with $\\rho$ an inhomogeneity parameter). Four types of inequivalent (super)conformal actions are thus obtained. With the exclusion of the homogeneous parabolic case, dimensional constants are present. Both the inhomogeneity and the insertion of $\\lambda$ generalize the construction of Papadopoulos [CQG 30 (2013) 075018; arXiv:1210.1719]. Inhomogeneous $D$-module reps are presented for the $d=1$ superconformal algebras $osp(1|2)$, $sl(2|1)$, $B(1,1)$ and $A(1,1)$. For centerless superVirasoro algebras $D$-module reps are presented (in the homogeneous case for ${\\cal N}=1,2,3,4$; in the inhomogeneous case for ${\\cal N}=1,2,3$). The four types of $d=1$ superconformal actions are derived for ${\\cal N}=1,2,4$ systems. When ${\\cal N}=4$, the homogeneously-induced actions are $D(2,1;\\alpha)$-invariant ($\\alpha$ is cri...

  6. Four types of (super)conformal mechanics: D-module reps and invariant actions

    Energy Technology Data Exchange (ETDEWEB)

    Holanda, N.L.; Toppan, F., E-mail: linneu@cbpf.br, E-mail: toppan@cbpf.br

    2014-03-15

    (Super)conformal mechanics in one dimension is induced by parabolic or hyperbolic/trigonometric transformations, either homogeneous (for a scaling dimension λor inhomogeneous (at λ = 0, with ρ an inhomogeneity parameter). Four types of inequivalent (super)conformal actions are thus obtained. With the exclusion of the homogeneous parabolic case, dimensional constants are present. Both the inhomogeneity and the insertion of λ generalize the construction of Papadopoulos [CQG 30 (2013) 075018; arXiv:1210.1719]. Inhomogeneous D-module reps are presented for the d = 1 superconformal algebras osp(1∣2), sl(2∣1), B(1, 1) and A(1, 1). For centerless super Virasoro algebras D-module reps are presented (in the homogeneous case for N = 1; 2; 3; 4; in the inhomogeneous case for N = 1, 2, 3). The four types of d = 1 superconformal actions are derived for N = 1, 2, 4 systems. When N = 4, the homogeneously-induced actions are D(2, 1; α)-invariant (α is critically linked to λ); the inhomogeneously-induced actions are A(1, 1)-invariant. In d = 2, for a single bosonic field, the homogeneous transformations induce a conformally invariant power-law action, while the inhomogeneous transformations induce the conformally invariant Liouville action. (author)

  7. La segunda república: un discurso esquivo en la izquierda colombiana

    Directory of Open Access Journals (Sweden)

    Miguel Antonio Espinosa Rico

    2013-01-01

    Full Text Available El artículo trata sobre el estado actual de la realidad de la izquierda colombiana y su relación con la crisis del estado moderno colombiano. Se argumenta con FalsBorda (2001 y otros, que la globalización constituye un tema central para la consolidación del capitalismo colombiano, considerando con los autores revisados que el Estado Moderno representa la estructura sobre la cual se yergue el capitalismo en Occidente, pero que en Latinoamérica, y en especial, en la República Neogranadina, sienta las bases para la perpetuación del sistema. Analiza el sistema de división territorial colombiano para establecer premisas de dominio del régimen capitalista sobre el ciudadano común. Se concluye que el nuevo país solo es posible con sueño de la izquierda de la Segunda República, que reivindica las regiones como pilar fundamental para la dignidad del pueblo colombiano.

  8. Postsymbiotic plasmid acquisition and evolution of the repA1-replicon in Buchnera aphidicola

    Science.gov (United States)

    Van Ham, Roeland C. H. J.; González-Candelas, Fernando; Silva, Francisco J.; Sabater, Beatriz; Moya, Andrés; Latorre, Amparo

    2000-01-01

    Buchnera aphidicola is an obligate, strictly vertically transmitted, bacterial symbiont of aphids. It supplies its host with essential amino acids, nutrients required by aphids but deficient in their diet of plant phloem sap. Several lineages of Buchnera show adaptation to their nutritional role in the form of plasmid-mediated amplification of key-genes involved in the biosynthesis of tryptophan (trpEG) and leucine (leuABCD). Phylogenetic analyses of these plasmid-encoded functions have thus far suggested the absence of horizontal plasmid exchange among lineages of Buchnera. Here, we describe three new Buchnera plasmids, obtained from species of the aphid host families Lachnidae and Pemphigidae. All three plasmids belong to the repA1 family of Buchnera plasmids, which is characterized by the presence of a repA1-replicon responsible for replication initiation. A comprehensive analysis of this family of plasmids unexpectedly revealed significantly incongruent phylogenies for different plasmid and chromosomally encoded loci. We infer from these incongruencies a case of horizontal plasmid transfer in Buchnera. This process may have been mediated by secondary endosymbionts, which occasionally undergo horizontal transmission in aphids. PMID:10984505

  9. RepExplore: addressing technical replicate variance in proteomics and metabolomics data analysis.

    Science.gov (United States)

    Glaab, Enrico; Schneider, Reinhard

    2015-07-01

    High-throughput omics datasets often contain technical replicates included to account for technical sources of noise in the measurement process. Although summarizing these replicate measurements by using robust averages may help to reduce the influence of noise on downstream data analysis, the information on the variance across the replicate measurements is lost in the averaging process and therefore typically disregarded in subsequent statistical analyses.We introduce RepExplore, a web-service dedicated to exploit the information captured in the technical replicate variance to provide more reliable and informative differential expression and abundance statistics for omics datasets. The software builds on previously published statistical methods, which have been applied successfully to biomedical omics data but are difficult to use without prior experience in programming or scripting. RepExplore facilitates the analysis by providing a fully automated data processing and interactive ranking tables, whisker plot, heat map and principal component analysis visualizations to interpret omics data and derived statistics. Freely available at http://www.repexplore.tk enrico.glaab@uni.lu Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press.

  10. Generation of RGB-D data for SLAM using robotic framework V-REP

    Science.gov (United States)

    Gritsenko, Pavel S.; Gritsenko, Igor S.; Seidakhmet, Askar Zh.; Abduraimov, Azizbek E.

    2017-09-01

    In this article, we will present a methodology to debug RGB-D SLAM systems as well as to generate testing data. We have created a model of a laboratory with an area of 250 m2 (25 × 10) with set of objects of different type. V-REP Microsoft Kinect sensor simulation model was used as a basis for robot vision system. Motion path of the sensor model has multiple loops. We have written a program in V-Rep native language Lua to record data array from the Microsoft Kinect sensor model. The array includes both RGB and Depth streams with full resolution (640 × 480) for every 10 cm of the path. The simulated path has absolute accuracy, since it is a simulation, and is represented by an array of transformation matrices (4 × 4). The length of the data array is 1000 steps or 100 m. The path simulates frequently occurring cases in SLAM, including loops. It is worth noting that the path was modeled for a mobile robot and it is represented by a 2D path parallel to the floor at a height of 40 cm.

  11. La “República de Colombia” del Libertador Simón Bolívar

    Directory of Open Access Journals (Sweden)

    Carlos Uribe Celis

    2013-01-01

    Full Text Available Este ensayo traza el recorrido hist rico de la Rep blica de Colombia como la visualiz , la program y le dio vida el Libertador Sim n Bol var. La Rep blica de Colombia de Bol var era la uni n de lo que despu s constituyeron tres rep blicas distintas, hoy conocidas como Venezuela, Colombia y Ecuador. Bol var aprovecha parcialmente una idea original de Miranda (la Colombeia del sue o continental del ?recursor?, y este ensayo ilustra la con?guraci n del proyecto bolivariano, desde sus comienzos ajenos hasta la disoluci n del mismo, lo que pr cticamente coincide con la muerte del Libertador. La historia de Colombia se ubica aqu en el contexto de la lucha bolivariana por la Independencia del norte y centro de Suram rica.

  12. fMRI repetition suppression: neuronal adaptation or stimulus expectation?

    Science.gov (United States)

    Larsson, Jonas; Smith, Andrew T

    2012-03-01

    Measurements of repetition suppression with functional magnetic resonance imaging (fMRI adaptation) have been used widely to probe neuronal population response properties in human cerebral cortex. fMRI adaptation techniques assume that fMRI repetition suppression reflects neuronal adaptation, an assumption that has been challenged on the basis of evidence that repetition-related response changes may reflect unrelated factors, such as attention and stimulus expectation. Specifically, Summerfield et al. (Summerfield C, Trittschuh EH, Monti JM, Mesulam MM, Egner T. 2008. Neural repetition suppression reflects fulfilled perceptual expectations. Nat Neurosci. 11:1004-1006) reported that the relative frequency of stimulus repetitions and non-repetitions influenced the magnitude of repetition suppression in the fusiform face area, suggesting that stimulus expectation accounted for most of the effect of repetition. We confirm that stimulus expectation can significantly influence fMRI repetition suppression throughout visual cortex and show that it occurs with long as well as short adaptation durations. However, the effect was attention dependent: When attention was diverted away from the stimuli, the effects of stimulus expectation completely disappeared. Nonetheless, robust and significant repetition suppression was still evident. These results suggest that fMRI repetition suppression reflects a combination of neuronal adaptation and attention-dependent expectation effects that can be experimentally dissociated. This implies that with an appropriate experimental design, fMRI adaptation can provide valid measures of neuronal adaptation and hence response specificity.

  13. ERIC-PCR typing and main drug resistance mechanism in 75 isolates of carbapenem-resistant Pseudomonas aeruginosa%75株碳青霉烯类耐药铜绿假单胞菌 ERIC-PCR菌种分型及其主要耐药机制研究

    Institute of Scientific and Technical Information of China (English)

    张勇; 刘爱胜; 文艳

    2014-01-01

    Objective To investigate the homology and main drug resistance mechanism of carbapenem-resistant Pseudomonas aeruginosa.Methods A total of 946 samples were collected,and carbapenem-resistant Pseudomonas aeruginosa were isolated.The drug sensitivity of carbapenem-resistant Pseudomonas aeruginosa were detected by agar dilution test.Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)was performed to analyze homology.The isolates were screened by metallo-beta-lactamae.The efflux pump of Pseudomonas aeruginosa resistant to carbapenem antibiotics was detected by efflux pump inhibitor test.Results A total of 75 isolates of carbapenem-resistant Pseudomonas aeruginosa were isolated.The ERIC-PCR homology analysis showed 8 types,in which A-type and B-type were the main types,accounting for 34.7% (26 isolates)and 22.7% (17 isolates), respectively.In the 75 isolates resistant to carbapenems,13 isolates were positive in metal enzyme test,accounting for 17.3%.MC207110 efflux pump inhibitor could make the minimal inhibitory concentration (MIC)of meropenem in 34 isolates being resistant to meropenem decreasing by 4 times or more,compared with single drug therapy,accounting for 63.0%.Conclusions There are mainly 2 kinds of cloning existing in Pseudomonas aeruginosa being resistant to carbapenem.The production of metallo-beta-lactamase and multi-drug resistant efflux system play important roles in the drug resistance of Pseudomonas aeruginosa.%目的:探讨碳青霉烯类耐药的铜绿假单胞菌同源性及其主要耐药机制。方法收集临床标本946份,分离出碳青霉烯类耐药的铜绿假单胞菌,采用琼脂稀释法进行体外药物敏感性试验;采用肠杆菌基因间重复一致序列为引物的聚合酶链反应(ERIC-PCR)进行耐药菌株同源性分析;筛选其金属β-内酰胺酶并测定主动外排系统表型来分析碳青霉烯类耐药的铜绿假单胞菌的耐药性。结果共检测出75株碳

  14. Desplazando al Rey en la escuela de la nueva nación mexicana: "el catecismo de República"

    Directory of Open Access Journals (Sweden)

    María Adelina ARREDONDO LÓPEZ

    2010-03-01

    Full Text Available RESUMEN: Este artículo estudia cómo un texto escolar, como el catecismo político de la nueva República de México, contribuye a desplazar de la escuela primaria la presencia colonial española, representada en el Rey, por nuevas imágenes de las instituciones de la naciente República de México. ABSTRACT: This paper studie how a textbook, the politic catechism of Mexican Republic, contribute to suplant the Spanish King instead the new images of the recent Mexican Republic during the emancipation process.

  15. Impact of Capsid Conformation and Rep-Capsid Interactions on Adeno-Associated Virus Type 2 Genome Packaging

    OpenAIRE

    Bleker, Svenja; Pawlita, Michael; Kleinschmidt, Jürgen A.

    2006-01-01

    Single-stranded genomes of adeno-associated virus (AAV) are packaged into preformed capsids. It has been proposed that packaging is initiated by interaction of genome-bound Rep proteins to the capsid, thereby targeting the genome to the portal of encapsidation. Here we describe a panel of mutants with amino acid exchanges in the pores at the fivefold axes of symmetry on AAV2 capsids with reduced packaging and reduced Rep-capsid interaction. Mutation of two threonines at the rim of the fivefol...

  16. Presencia y bioacumulación de productos de cuidado personal en corales marinos de República Dominicana

    OpenAIRE

    De León Peguero, Ana Esther

    2016-01-01

    El objetivo del proyecto a desarrollar es determinar la presencia de determinados aditivos orgánicos que se utilizan en productos de cuidado personal como champú, gel de baño, maquillaje, cremas solares, etc, en aguas y arrecifes de coral de la República Dominicana. Recientemente se ha documentado que la presencia de este tipo de contaminantes es responsable del blanqueamiento de los corales en zonas del Pacífico y dada la preocupación creciente en la República. Dominicana por el mal estado ...

  17. Em busca do despertar: o fortalecimento da sociedade civil angolana após a Segunda República

    Directory of Open Access Journals (Sweden)

    Rodrigo de Souza Pain

    2006-01-01

    passagem da Primeira República, caracterizada por um Estado de Partido Único, por uma economia planificada e inspirada nos ideais marxistas e leninistas; para a Segunda República, ou seja, para uma economia de mercado, multipartidária, explicitando as adversidades advindas das diversas realidades sociais, políticas e culturais do espaço territorial angolano resultante do processo de colonização, para a formação e consolidação da democracia e da sociedade civil em Angola.

  18. Iberia : capital federal de la IIª República Española (Un proyecto de Rubio i Tuduri

    Directory of Open Access Journals (Sweden)

    Carlos Sambricio

    1996-01-01

    Full Text Available A los pocos meses de proclamarse, en 1931, la II República Española, Nicolás Rubio i Tuduri presentaba —en el marco de la Exposición organizada por la Asociación de Arquitectos de Cataluña, celebrada en la barcelonesa Galería Maragall— una propuesta un tanto sorprendente: el proyecto de una nueva Capital Federal de la República Española que él denominaba Iberia.

  19. A repetitively pulsed xenon chloride excimer laser with all ferrite magnetic cores (AFMC) based all solid state exciter

    Science.gov (United States)

    Benerji, N. S.; Varshnay, N. K.; Ghodke, D. V.; Singh, A.

    2016-10-01

    Performance of repetitively pulsed xenon chloride excimer laser (λ~308 nm) with solid state pulser consisting of magnetic pulse compression circuit (MPC) using all ferrite magnetic cores (AFMC) is reported. Laser system suitable for 100 Hz operation with inbuilt pre-ionizer, compact gas circulation and cooling has been developed and presented. In this configuration, high voltage pulses of ~8 μs duration are compressed to ~100 ns by magnetic pulse compression circuit with overall compression factor of ~80. Pulse energy of ~18 J stored in the primary capacitor is transferred to the laser head with an efficiency of ~85% compared to ~70% that is normally achieved in such configurations using annealed met-glass core. This is a significant improvement of about 21%. Maximum output laser pulse energy of ~100 mJ was achieved at repetition rate of 100 Hz with a typical pulse to pulse energy stability of ±5% and laser pulse energy of 150 mJ was generated at low rep-rate of ~40 Hz. This exciter uses a low current and low voltage solid state switch (SCR) that replaces high voltage and high current switch i. e, thyratron completely. The use of solid state exciter in turn reduces electromagnetic interference (EMI) effects particularly in excimer lasers where high EMI is present due to high di/dt. The laser is focused on a thin copper sheet for generation of micro-hole and the SEM image of the generated micro hole shows the energy stability of the laser at high repetition rate operation. Nearly homogeneous, regular and well developed xenon chloride (XeCl) laser beam spot was achieved using the laser.

  20. Escaping institutionality: rebellion and gendered space in Eric, or Little by Little and A Little Princess

    Directory of Open Access Journals (Sweden)

    Kim, Soyoun

    2015-06-01

    Full Text Available By the convention of nineteenth-century school narratives, students are supposed to grow up in the school while their characters are being molded by the standardized educational system. Eric, or Little by Little and A Little Princess both resist the very idea of linear growth that is a trope of the Bildungsroman through endowing their protagonists with the rebelliousness and outsider status of the unruly child. This essay examines how Eric Williams and Sara Crewe, disobedient students who leave their schools after deriving no benefit from the institutional education, illuminate the ways in which institutionality is incorporated with the gendered agendas of nineteenth-century England. Eric and Sara both challenge the authority of the central adult in each tale by exploiting and changing the interior space of the schools; not only the inner structures of each school but also the connection/disconnection between school and home differ in the two stories. My proposition is that in the nineteenth-century school narratives discipline and character-building in the institution are not viewed as inevitable in reaching adulthood for girls, while for boys there is neither exit from the institutionality nor way to recover their lost home and childhood.

  1. Storytelling and Repetitive Narratives for Design Empathy

    DEFF Research Database (Denmark)

    Fritsch, Jonas; Judice, Andrea; Soini, Katja

    2007-01-01

    Today it is widely established in design research that empathy is an important part of creating a true understanding of user experience as a resource for design. A typical challenge is how to transmit the feeling of empathy acquired by user studies to designers who have not participated in the user...... study. In this paper, we show how we attained an empathic understanding through storytelling and aroused empathy to others using repetitive narratives in an experimental presentation bringing forth factual, reflective and experiential aspects of the user information. Taking as a starting point our...... experiences with the design project Suomenlinna Seclusive, we conclude with the potential of using narratives for invoking design empathy....

  2. A miniature high repetition rate shock tube.

    Science.gov (United States)

    Tranter, R S; Lynch, P T

    2013-09-01

    A miniature high repetition rate shock tube with excellent reproducibility has been constructed to facilitate high temperature, high pressure, gas phase experiments at facilities such as synchrotron light sources where space is limited and many experiments need to be averaged to obtain adequate signal levels. The shock tube is designed to generate reaction conditions of T > 600 K, P shock waves with predictable characteristics are created, repeatably. Two synchrotron-based experiments using this apparatus are also briefly described here, demonstrating the potential of the shock tube for research at synchrotron light sources.

  3. Storytelling and Repetitive Narratives for Design Empathy

    DEFF Research Database (Denmark)

    Fritsch, Jonas; Judice, Andrea; Soini, Katja

    2007-01-01

    Today it is widely established in design research that empathy is an important part of creating a true understanding of user experience as a resource for design. A typical challenge is how to transmit the feeling of empathy acquired by user studies to designers who have not participated in the user...... study. In this paper, we show how we attained an empathic understanding through storytelling and aroused empathy to others using repetitive narratives in an experimental presentation bringing forth factual, reflective and experiential aspects of the user information. Taking as a starting point our...... experiences with the design project Suomenlinna Seclusive, we conclude with the potential of using narratives for invoking design empathy....

  4. The repetitive component of the sunflower genome

    Directory of Open Access Journals (Sweden)

    T. Giordani

    2014-08-01

    Full Text Available The sunflower (Helianthus annuus and species belonging to the genus Helianthus are emerging as a model species and genus for a number of studies on genome evolution. In this review, we report on the repetitive component of the H. annuus genome at the biochemical, molecular, cytological, and genomic levels. Recent work on sunflower genome composition is described, with emphasis on different types of repeat sequences, especially LTR-retrotransposons, of which we report on isolation, characterisation, cytological localisation, transcription, dynamics of proliferation, and comparative analyses within the genus Helianthus.

  5. The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic reticulum membrane for degradation of misfolded proteins.

    Directory of Open Access Journals (Sweden)

    Louise Madsen

    Full Text Available The protein known as p97 or VCP in mammals and Cdc48 in yeast is a versatile ATPase complex involved in several biological functions including membrane fusion, protein folding, and activation of membrane-bound transcription factors. In addition, p97 plays a central role in degradation of misfolded secretory proteins via the ER-associated degradation pathway. This functional diversity of p97 depends on its association with various cofactors, and to further our understanding of p97 function it is important that these cofactors are identified and analyzed. Here, we isolate and characterize the human protein named Rep8 or Ubxd6 as a new cofactor of p97. Mouse Rep8 is highly tissue-specific and abundant in gonads. In testes, Rep8 is expressed in post-meiotic round spermatids, whereas in ovaries Rep8 is expressed in granulosa cells. Rep8 associates directly with p97 via its UBX domain. We show that Rep8 is a transmembrane protein that localizes to the ER membrane with its UBX domain facing the cytoplasm. Knock-down of Rep8 expression in human cells leads to a decreased association of p97 with the ER membrane and concomitantly a retarded degradation of misfolded ER-derived proteasome substrates. Thus, Rep8 tethers p97 to the ER membrane for efficient ER-associated degradation.

  6. Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco.

    Science.gov (United States)

    Shivaprasad, Padubidri V; Thillaichidambaram, P; Balaji, Vasudevan; Veluthambi, Karuppannan

    2006-12-01

    Mungbean yellow mosaic virus-Vigna (MYMV-Vig) is a bipartite geminivirus that causes a severe yellow mosaic disease in blackgram. An assay was developed to study MYMV-Vig replication by agroinoculation of tobacco leaf discs with partial dimers of the virus. This assay, in a non-host model plant, was used to evaluate pathogen-derived resistance contributed by MYMV-Vig genes in transgenic plants. Viral DNA accumulation was optimum in tobacco leaf discs cultured for 10 days after infection with Agrobacterium tumefaciens strain Ach5 containing partial dimers of both DNA A and DNA B of MYMV-Vig. Transgenic tobacco plants with MYMV-Vig genes for coat protein (CP), replication-associated protein (Rep)-sense, Rep-antisense, truncated Rep (T-Rep), nuclear shuttle protein (NSP) and movement protein (MP) were generated. Leaf discs from transgenic tobacco plants, harbouring MYMV-Vig genes, were agroinoculated with partial dimers of MYMV-Vig and analyzed for viral DNA accumulation. The leaf discs from transgenic tobacco plants harbouring CP and MP genes supported the accumulation of higher levels of MYMV-Vig DNA. However, MYMV-Vig accumulation was inhibited in one transgenic plant harbouring the Rep-sense gene and in two plants harbouring the T-Rep gene. Northern analysis of these plants revealed a good correlation between expression of Rep or T-Rep genes and inhibition of MYMV-Vig accumulation.

  7. Setting the stage for the EPOS ERIC: Integration of the legal, governance and financial framework

    Science.gov (United States)

    Atakan, Kuvvet; Bazin, Pierre-Louis; Bozzoli, Sabrina; Freda, Carmela; Giardini, Domenico; Hoffmann, Thomas; Kohler, Elisabeth; Kontkanen, Pirjo; Lauterjung, Jörn; Pedersen, Helle; Saleh, Kauzar; Sangianantoni, Agata

    2017-04-01

    EPOS - the European Plate Observing System - is the ESFRI infrastructure serving the need of the solid Earth science community at large. The EPOS mission is to create a single sustainable, and distributed infrastructure that integrates the diverse European Research Infrastructures for solid Earth science under a common framework. Thematic Core Services (TCS) and Integrated Core Services (Central Hub, ICS-C and Distributed, ICS-D) are key elements, together with NRIs (National Research Infrastructures), in the EPOS architecture. Following the preparatory phase, EPOS has initiated formal steps to adopt an ERIC legal framework (European Research Infrastructure Consortium). The statutory seat of EPOS will be in Rome, Italy, while the ICS-C will be jointly operated by France, UK and Denmark. The TCS planned so far cover: seismology, near-fault observatories, GNSS data and products, volcano observations, satellite data, geomagnetic observations, anthropogenic hazards, geological information modelling, multiscale laboratories and geo-energy test beds for low carbon energy. In the ERIC process, EPOS and all its services must achieve sustainability from a legal, governance, financial, and technical point of view, as well as full harmonization with national infrastructure roadmaps. As EPOS is a distributed infrastructure, the TCSs have to be linked to the future EPOS ERIC from legal and governance perspectives. For this purpose the TCSs have started to organize themselves as consortia and negotiate agreements to define the roles of the different actors in the consortium as well as their commitment to contribute to the EPOS activities. The link to the EPOS ERIC shall be made by service agreements of dedicated Service Providers. A common EPOS data policy has also been developed, based on the general principles of Open Access and paying careful attention to licensing issues, quality control, and intellectual property rights, which shall apply to the data, data products

  8. An investigation of the subtype diversity of clinical isolates of Irish Clostridium difficile ribotypes 027 and 078 by repetitive-extragenic palindromic PCR.

    LENUS (Irish Health Repository)

    Solomon, K

    2011-08-01

    A repetitive-extragenic palindromic PCR (rep-PCR) subtyping method (DiversiLab) in conjunction with ribotyping, toxinotyping and antimicrobial-susceptibility testing was used to detect subtypes within Clostridium difficile ribotypes 027 and 078. Clinical isolates of ribotypes 027 (toxinotype III) (n = 30) and 078 (toxinotype V) (n = 23) were provided by health-care facilities across the Republic of Ireland over 2 months in 2006 and 1 month in 2009. Ribotype 027 isolates were significantly more related to each other (9 different subtype profiles) when compared to ribotype 078 isolates (14 different profiles) (P = 0.001; cut-off >90 % similarity). Almost half of ribotype 078 isolates (45.5 %) showed no relationship to each other. The clonality of ribotype 027 isolates suggests effective adaptation to the human niche, whereas the considerable genetic diversity within ribotype 078 isolates suggests that they may have originated from a variety of sources. Subtyping correlated well with antimicrobial susceptibility, in particular clindamycin susceptibility for ribotype 027, but diverse antimicrobial-susceptibility profiles were seen in ribotype 078 isolates, even within a single health-care facility. Between 2006 and 2009, a change in the predominant subtype of ribotype 027 was seen, with the recent clone representing half of all ribotype 027 isolates studied. This strain exhibited 89 % similarity to a rep-PCR profile of the North American NAP-1 strain.

  9. The role of short-term memory impairment in nonword repetition, real word repetition, and nonword decoding: A case study.

    Science.gov (United States)

    Peter, Beate

    2017-09-21

    In a companion study, adults with dyslexia and adults with a probable history of childhood apraxia of speech showed evidence of difficulty with processing sequential information during nonword repetition, multisyllabic real word repetition and nonword decoding. Results suggested that some errors arose in visual encoding during nonword reading, all levels of processing but especially short-term memory storage/retrieval during nonword repetition, and motor planning and programming during complex real word repetition. To further investigate the role of short-term memory, a participant with short-term memory impairment (MI) was recruited. MI was confirmed with poor performance during a sentence repetition and three nonword repetition tasks, all of which have a high short-term memory load, whereas typical performance was observed during tests of reading, spelling, and static verbal knowledge, all with low short-term memory loads. Experimental results show error-free performance during multisyllabic real word repetition but high counts of sequence errors, especially migrations and assimilations, during nonword repetition, supporting short-term memory as a locus of sequential processing deficit during nonword repetition. Results are also consistent with the hypothesis that during complex real word repetition, short-term memory is bypassed as the word is recognized and retrieved from long-term memory prior to producing the word.

  10. 环境因素对大肠杆菌REP-PCR指纹图谱稳定性影响研究%Effect of environmental factors of REP-PCR fingerprinting patterns stability of Escherichia coil

    Institute of Scientific and Technical Information of China (English)

    韩彦垒; 梁斌; 李冬梅; 苏洁; 关道明; 王耀兵

    2011-01-01

    Escherichia coli is an important indicator in fecal pollution, The E. Coli DN A fingerprinting stability is to restrict one of identifying techniques development of the microbial polluted sources. In order to solve this problem, the E. Coli K12( K12CFP) , which the green fluorescent protein (CFP) plasmid was transformed, were chose to make a research. Its DNA fingerprinting stability was under different environmental factors (temperature,sunlight time and salinity). The results showed that the green fluorescent protein (CFP) did not affect the REP-PCR fingerprinting patterns of E. Coli K12 and the REP-PCR fingerprinting pattern is highly stable under the different environmental factors, temperature, sunlight time and salinity.%大肠杆菌作为粪便污染指示生物,其DNA指纹图谱稳定性是制约微生物污染源识别技术发展的瓶颈之一.本研究采用绿色荧光蛋白基因(GFP)标记后的Escherichia coli K12(K12GFP)为受试菌株,利用REP-PCR方法,对大肠杆菌在不同温度、光照、盐度条件下其DNA指纹图谱的稳定性进行了研究.结果表明,绿色荧光蛋白基因的标记不影响E.coli K12的REP-PCR指纹图谱;且大肠杆菌在不同温度、光照、盐度条件下REP-PCR指纹图谱具有高度的稳定性.

  11. Eficacia publicitaria: El caso de IKEA y 'La República Independiente de Tu Casa'

    OpenAIRE

    Núñez Ramírez, Marta

    2014-01-01

    L'estudi pretén aprofundir en l'àmbit de l'avaluació de l'eficàcia publicitària, a través de l'anàlisi d'un cas concret: l'anunci d'IKEA 'Benvingut a la República Independent de casa teva' de l'any 2006. Primerament, s'intenta explicar en què consisteix el concepte d'eficàcia, es reflexiona sobre la seva relació o no amb les vendes que aconsegueix generar una marca, i s'obren vies sobre criteris d'avaluació útils per a la investigació. S'arriba a una primera observació: la importància dels ob...

  12. Triatoma baccalaureatus: sobre a crise do bacharelismo na Primeira República

    Directory of Open Access Journals (Sweden)

    Ricardo Sontag

    2010-07-01

    Full Text Available Este trabalho pretende abordar a crise do bacharelismo (hegemônico durante o Império nas primeiras décadas do século XX, principalmente por meio da literatura, relacionando-a com as transformações que estavam ocorrendo no ensino e no saber jurídico. Ao longo da Primeira República, à medida que outras figuras passam a ganhar espaço no cenário público (engenheiros, sanitaristas, etc., o bacharelismo passa a ser duramente questionado, aprofundando as críticas que já vinham sendo levadas a cabo no final do século XIX pelo positivismo cientificista; a partir disso, começa a se formar a cultura do tecnicismo jurídico. Palavras-chave: História do Direito. Bacharelismo. Positivismo cientificista. Tecnicismo.

  13. Agripino Nazareth e o movimento operário da Primeira República

    Directory of Open Access Journals (Sweden)

    Aldrin Armstrong Silva Castellucci

    2012-12-01

    Full Text Available Agripino Nazareth (1886-1961 se destacou como liderança socialista do movimento operário brasileiro ao longo da Primeira República. Depois de 1930, passou a integrar os quadros do Ministério do Trabalho e da Justiça do Trabalho, aposentando-se como Procurador do Trabalho no início de 1960 e falecendo no dia 1º de agosto de 1961. Neste artigo, analisaremos o primeiro momento de sua trajetória, quando se associou a outros militantes das mais variadas correntes político-ideológicas, envolvendo-se em movimentos de diversos matizes e assumindo diferentes compromissos.

  14. Site-specific integration by the adeno-associated virus rep protein.

    Science.gov (United States)

    Recchia, Alessandra; Mavilio, Fulvio

    2011-10-01

    Inserting genetic information at precise locations into the human genome has been the goal of gene transfer technology for almost two decades. The spectacular progress of mammalian genetics has led to the development of technology for genome editing and homologous recombination in human somatic cells that is finally approaching efficiency compatible with clinical application. Site-specific integration, or the insertion of genes at known locations by enzymes with target recognition capacity, has progressed slowly but steadily in recent years, and could very well be the basis of the next generation of gene transfer technology. This review focuses on the use of Rep, the replicase/integrase of the adeno-associated virus (AAV), to insert genes at the natural AAV integration site on human chromosome 19. This region (AAVS1) has characteristics that make it an ideal target for somatic transgenesis.

  15. A paradiplomacia financeira no Brasil da República Velha, 1890-1930

    Directory of Open Access Journals (Sweden)

    José Nelson Bessa Maia

    2012-01-01

    Full Text Available Neste artigo, visa-se a analisar um caso de extroversão de governos estaduais no campo do financiamento externo no período da chamada República Velha (1890-1930 no Brasil. Com base na definição de "paradiplomacia financeira", argumenta que a experiência histórica brasileira nesse aspecto teria sido um caso típico de ação paradiplomática. Amplo levantamento estatístico dos empréstimos externos por estados, o resgate das fontes documentais primárias sobre o assunto e a contextualização histórica pertinente dão sustentação à caracterização do período como uma fase de paradiplomacia financeira precoce.

  16. A paradiplomacia financeira no Brasil da República Velha, 1890-1930

    OpenAIRE

    José Nelson Bessa Maia; José Flávio Sombra Saraiva

    2012-01-01

    Neste artigo, visa-se a analisar um caso de extroversão de governos estaduais no campo do financiamento externo no período da chamada República Velha (1890-1930) no Brasil. Com base na definição de "paradiplomacia financeira", argumenta que a experiência histórica brasileira nesse aspecto teria sido um caso típico de ação paradiplomática. Amplo levantamento estatístico dos empréstimos externos por estados, o resgate das fontes documentais primárias sobre o assunto e a contextualização históri...

  17. A phonetic approach to consonant repetition in early words.

    Science.gov (United States)

    Kim, Namhee; Davis, Barbara L

    2015-08-01

    The goal of this study was to evaluate movement-based principles for understanding early speech output patterns. Consonant repetition patterns within children's actual productions of word forms were analyzed using spontaneous speech data from 10 typically developing American-English learning children between 12 and 36 months of age. Place of articulation, word level patterns, and developmental trends in CVC and CVCV repeated word forms were evaluated. Labial and coronal place repetitions dominated. Regressive repetition (e.g., [gag] for "dog") occurred frequently in CVC but not in CVCV word forms. Consonant repetition decreased over time. However, the children produced sound types available reported as being within young children's production system capabilities in consonant repetitions in all time periods. Findings suggest that a movement-based approach can provide a framework for comprehensively characterizing consonant place repetition patterns in early speech development.

  18. Repetition and Reactance in Graham’s "Underneath" Poems

    Directory of Open Access Journals (Sweden)

    Roghayeh Farsi

    2017-09-01

    Full Text Available The present paper gives a detailed analysis and interpretation of 16 poems in Jorie Graham's collection, Swarm (2000, which bear "UNDERNEATH" as their main titles. The poems are marked with different types of repetition such as graphological repetition, word, phrase, and sentential repetition, semantic repetition, and syntactic repetition. The study draws on Lakoff and Johnson's theories on metaphor and Brehm and Brehm’s reactance theory. It is argued "underneath" is a conceptual (orientational metaphor which signifies a state of being limited, lack of control and freedom, and loss of power. The paper investigates the speaker's reactant behavior in "Underneath" poems, seeking a way to restore her lost freedom. Reactance behaviors can be skepticism, inertia, aggression, and resistance. It is concluded despite her thematic inertia, representing her submission to the oppressed state, her stylistic reactance reflected in repetitions, innovations, and disruptive diction stands for her attempts to regain her lost control.

  19. Determination of in vitro relative potency (REP) values for mono-ortho polychlorinated biphenyls after purification with active charcoal

    NARCIS (Netherlands)

    Peters, A.K.; Leonards, P.E.G.; Zhao, B.; Bergman, A.; Denison, M.S.; Berg, van den M.

    2006-01-01

    The TEF system for dioxin-like compounds has included assignment of TEF values for mono-ortho polychlorinated biphenyls (MO-PCBs). Small traces of aryl hydrocarbon receptor (AhR)-active impurities could result in artifactually higher relative potency (REP) values. MO-PCBs -105, -118, -156, and -167

  20. 78 FR 53774 - Guide for the Evaluation of Alert and Notification Systems for Nuclear Power Plants, FEMA-REP-10...

    Science.gov (United States)

    2013-08-30

    ... not available when FEMA REP-10 was first developed, such as smartphones and social media. Removes... Design Report) and planning aspects (ANS/Communication Plans) demonstrating the achievement of minimally... proposed policy does not have the force or effect of law. FEMA seeks comment on the proposed policy,...

  1. The OSIRIS Weight of Evidence approach: ITS for the endpoints repeated-dose toxicity (RepDose ITS)

    NARCIS (Netherlands)

    Tluczkiewicz, I.; Batke, M.; Kroese, D.; Buist, H.; Aldenberg, T.; Pauné, E.; Grimm, H.; Kühne, R.; Schüürmann, G.; Mangelsdorf, I.; Escher, S.E.

    2013-01-01

    In the FP6 European project OSIRIS, Integrated Testing Strategies (ITSs) for relevant toxicological endpoints were developed to avoid new animal testing and thus to reduce time and costs. The present paper describes the development of an ITS for repeated-dose toxicity called RepDose ITS which evalua

  2. Open-Source Wax RepRap 3-D Printer for Rapid Prototyping Paper-Based Microfluidics.

    Science.gov (United States)

    Pearce, J M; Anzalone, N C; Heldt, C L

    2016-08-01

    The open-source release of self-replicating rapid prototypers (RepRaps) has created a rich opportunity for low-cost distributed digital fabrication of complex 3-D objects such as scientific equipment. For example, 3-D printable reactionware devices offer the opportunity to combine open hardware microfluidic handling with lab-on-a-chip reactionware to radically reduce costs and increase the number and complexity of microfluidic applications. To further drive down the cost while improving the performance of lab-on-a-chip paper-based microfluidic prototyping, this study reports on the development of a RepRap upgrade capable of converting a Prusa Mendel RepRap into a wax 3-D printer for paper-based microfluidic applications. An open-source hardware approach is used to demonstrate a 3-D printable upgrade for the 3-D printer, which combines a heated syringe pump with the RepRap/Arduino 3-D control. The bill of materials, designs, basic assembly, and use instructions are provided, along with a completely free and open-source software tool chain. The open-source hardware device described here accelerates the potential of the nascent field of electrochemical detection combined with paper-based microfluidics by dropping the marginal cost of prototyping to nearly zero while accelerating the turnover between paper-based microfluidic designs. © 2016 Society for Laboratory Automation and Screening.

  3. La lucha contra la discriminación y la apatridia en la República Dominicana

    OpenAIRE

    Bridget WOODING

    2009-01-01

    Tras muchas décadas de migración irregular de haitianos que llegan a la República Dominicana para asentarse y trabajar, existe una población numerosa en situación incierta expuesta a frecuentes discriminaciones y abusos de los derechos humanos.

  4. Mida on vaja teha õpilaskodude arendamiseks? / Ale Sprenk, Karin Saare, Merike Mändla, Mailis Reps...[jt.

    Index Scriptorium Estoniae

    2008-01-01

    Küsimusele vastavad Krabi põhikooli direktor, õpilaskodu mõtte algataja Ale Sprenk, Kasari põhikooli direktor Karin Saare, haridus- ja teadusministeeriumi üldharidusosakonna peaekspert Merike Mändla, riigikogu liige, endine haridusminister Mailis Reps ja Otepää gümnaasiumi direktor Aivo Meema

  5. Feature selection and data sampling methods for learning reputation dimensions: The University of Amsterdam at RepLab 2014

    NARCIS (Netherlands)

    Gârbacea, C.; Tsagkias, M.; de Rijke, M.

    2014-01-01

    We report on our participation in the reputation dimension task of the CLEF RepLab 2014 evaluation initiative, i.e., to classify social media updates into eight predefined categories. We address the task by using corpus-based methods to extract textual features from the labeled training data to

  6. Naturaleza jurídica de la Contraloría General de la República

    OpenAIRE

    Murillo, Mauro

    2014-01-01

    Todavía reina una confusión acerca de la naturaleza jurídica de la ContraloríaGeneral  de la República, pese a los 15 años de vigencia del texto constitucional, cuya insuficiente redacción es obviamente la causante del problema.

  7. DEMO_REP: A LABS TOOL FOR TEACHING DATA REPLICATION / DEMO_REP: HERRAMIENTA PARA LABORATORIOS EN LA ENSEÑANZA DE RÉPLICA DE DATOS

    Directory of Open Access Journals (Sweden)

    Ileane Martí Pérez

    2013-02-01

    Full Text Available The process of training professionals in information technologies is a key aspect in the development of the country. University Informatics Science is one of the main centers dedicated to this process, also has postgraduate training programs to further advanced topics not mentioned in the pregraduate. One such program is the "DIPLOMADO EN TECNOLOGÍAS DE BASES DE DATOS POSTGRESQL," which has a number of courses to obtain expertise in the subject of databases in PostgreSQL Manager. In this paper show how affects in the understanding of the process of data replication, the use of the software tool "Demo_rep" for laboratory exercises replication. It also describes the main features of software tool.RESUMENEl proceso de formación de los profesionales en tecnologías de la informática es un aspecto clave en el desarrollo del país. La Universidad de las Ciencias Informáticas es uno de los principales centros que se dedica a este proceso, la misma consta de programas de formación postgraduada para profundizar en temas que no aparecen incluidos en los programas analíticos de las asignaturas de pregrado. Uno de estos programas es el “DIPLOMADO EN TECNOLOGÍAS DE BASES DE DATOS POSTGRESQL”, el cual cuenta con una serie de cursos para lograr experticia en la temática de las bases de datos en el gestor PostgreSQL. En el presente trabajo se muestra cómo incide en la comprensión del proceso de la replicación de datos, la utilización de una herramienta informática “Demo_rep” para realizar los ejercicios de laboratorio de réplica. Se describe además las principales funcionalidades de la misma.

  8. Phylogenetic relationships, virulence factors and Rep-PCR epidemiological analysis of E. coli from human sources

    Directory of Open Access Journals (Sweden)

    Simona Caroppo

    2010-06-01

    Full Text Available The potential of Escherichia coli to cause of extra-intestinal infections was studied on a group of 94 clinical isolates. In this work, 32 E. coli isolates from urinary tract infections, 25 from bacteraemia, 12 from low respiratory tract infections, and 25 from the normal commensal flora were characterized for the phylogenetic type, the virulence factors (VFs carriage and the Rep-PCR clonal composition.The B2 phylogenetic type was predominant among the urinary isolates (59%, the B2 and D strains among the haematic isolates (32% and 32%.The A phylogenetic type was predominant among the commensal and the respiratory isolates (52% and 58% respectively.The distribution of the B2 type strains among the urinary isolates and of the D type strains among the faecal isolates was suggesting a urinary-origin for the B2 phylogenetic type isolates found in the blood and a direct faecal derivation for the haematic isolates with D phylogenetic type.Twenty-nine VFs were analyzed.The B2 and D type strains carried a higher burden of VFs than the A and B1 phylogenetic type strains (average of VFs/strain = 8 vs 3. Some of the VFs were homogeneously distributed among the phylogenetic types (fimH, iutA, fyuA, traT. The PAI, papGII, ibeA, KpsMTIII were exclusive of B2 and D phylogenetic type strains, while sfa/foc, focG, cnf1, hlyA and rfc were exclusively observed among the B2 type strains.The clustering analysis by Rep-PCR distinguished two groups of strains, the first including 96.77% of B2 and D type strains, while the second encompassing 91,5% of A and B1 type strains.

  9. Los médicos del exilio repúblicano en Venezuela

    Directory of Open Access Journals (Sweden)

    José Francisco Tinao Martín-Peña

    2009-11-01

    Full Text Available La guerra civil española provocó el exilio de un importante número de científicos e intelectuales. Dentro de ellos numerosos médicos saldrían de España obligados por su defensa de la República o su ideología democrática. Una gran mayoría se establecieron en América Latina. Venezuela es el segundo país donde más facultativos acoge, tras México. En el presente trabajo se analiza cuales fueron las circunstancias históricas que permitieron esta acogida, y se hace referencia a alguna de las personalidades y sus campos de trabajo en su nuevo país de acogida. Dentro de la historia social de la medicina española existe un espacio vacío sobre el exilio médico y sobre la represión interior ejercida físicamente o simplemente como depurados de numerosos médicos que se vincularon de distinto modo con los ideales de modernidad de la República Española.______________ABSTRACT:The Spanish Civil War led to the exile of a large number of scientists and intellectuals. Among them, many doctors escaped from Spain because of their defense of the Republic or their democratic ideology. A large majority were established in Latin America. Venezuela is the second country of welcome, after Mexico. In this paper we analyze what were the historical circumstances that allowed this host, and it is made reference to some personalities and their fields of work in their new host country. Within the social history of Spanish medicine, there is an empty space on the exile of this category and their internal repression or the purged of many doctors linked to the ideals of modernity of the Spanish Republic.

  10. Engineered bacterial hydrophobic oligopeptide repeats in a synthetic yeast prion, [REP-PSI+

    Directory of Open Access Journals (Sweden)

    Fátima eGasset-Rosa

    2015-04-01

    Full Text Available The yeast translation termination factor Sup35p, by aggregating as the [PSI+] prion, enables ribosomes to read-through stop codons, thus expanding the diversity of the Saccharomyces cerevisiae proteome. Yeast prions are functional amyloids that replicate by templating their conformation on native protein molecules, then assembling as large aggregates and fibers. Prions propagate epigenetically from mother to daughter cells by fragmentation of such assemblies. In the N-terminal prion-forming domain, Sup35p has glutamine/asparagine-rich oligopeptide repeats (OPRs, which enable propagation through chaperone-elicited shearing. We have engineered chimeras by replacing the polar OPRs in Sup35p by up to five repeats of a hydrophobic amyloidogenic sequence from the synthetic bacterial prionoid RepA-WH1. The resulting hybrid, [REP-PSI+], i was functional in a stop codon read-through assay in S. cerevisiae; ii generates weak phenotypic variants upon both its expression or transformation into [psi-] cells; iii these variants correlated with high molecular weight aggregates resistant to SDS during electrophoresis; and iv according to fluorescence microscopy, the fusion of the prion domains from the engineered chimeras to the reporter protein mCherry generated perivacuolar aggregate foci in yeast cells. All these are signatures of bona fide yeast prions. As assessed through biophysical approaches, the chimeras assembled as oligomers rather than as the fibers characteristic of [PSI+]. These results suggest that it is the balance between polar and hydrophobic residues in OPRs what determines prion conformational dynamics. In addition, our findings illustrate the feasibility of enabling new propagation traits in yeast prions by engineering OPRs with heterologous amyloidogenic sequence repeats.

  11. A Primeira República Portuguesa (1910-1926: partidos e sistema político

    Directory of Open Access Journals (Sweden)

    Baiôa, Manuel

    2014-04-01

    Full Text Available For historical, organisational, clientelist, violent and constitutional reasons the Portuguese Republican Party became the dominant party in the political system of the Portuguese First Republic (1910-1926. The opposition felt alternation via elections would be difficult to achieve so resorted to violent and unconstitutional practices to reach government. The Portuguese political parties modernised themselves in this period, but failed to turn into mass parties. In major cities they acted as a cadre party and in the rural area as a party of notables. The First Republic failed to take solid steps to democratise the political system were And Portugal remained tied to the liberal and elitist tradition of the nineteenth century, although in a republican version.El Partido Republicano Portugués se convirtió en el partido dominante del sistema político de la Primera República Portuguesa (1910-1926 debido a causas históricas, organizacionales, clientelistas, violentas y constitucionales. La oposición sentía que difícilmente conseguiría la alternancia política a través de elecciones, y por lo tanto, recurre a prácticas violentas e inconstitucionales para llegar al gobierno. Los partidos políticos portugueses se modernizaron en este período, pero no se convirtieron en partidos de masas. En las principales ciudades actuaban como partidos de cuadros y en las zonas rurales como partidos de notables. Durante la Primera República no se dieron pasos consistentes para democratizar el sistema político. Portugal se mantuvo ancorado a la tradición liberal y elitista del siglo XIX, aunque en una versión republicana.

  12. FEMA Hazard Mitigation Assistance Repetitive Flood Claims (RFC) Data

    Data.gov (United States)

    Department of Homeland Security — This dataset contains closed and obligated projects funded under the following Hazard Mitigation Assistance (HMA) grant programs: Repetitive Flood Claims (RFC). The...

  13. A review of neuroimaging findings in repetitive brain trauma.

    Science.gov (United States)

    Koerte, Inga K; Lin, Alexander P; Willems, Anna; Muehlmann, Marc; Hufschmidt, Jakob; Coleman, Michael J; Green, Isobel; Liao, Huijun; Tate, David F; Wilde, Elisabeth A; Pasternak, Ofer; Bouix, Sylvain; Rathi, Yogesh; Bigler, Erin D; Stern, Robert A; Shenton, Martha E

    2015-05-01

    Chronic traumatic encephalopathy (CTE) is a neurodegenerative disease confirmed at postmortem. Those at highest risk are professional athletes who participate in contact sports and military personnel who are exposed to repetitive blast events. All neuropathologically confirmed CTE cases, to date, have had a history of repetitive head impacts. This suggests that repetitive head impacts may be necessary for the initiation of the pathogenetic cascade that, in some cases, leads to CTE. Importantly, while all CTE appears to result from repetitive brain trauma, not all repetitive brain trauma results in CTE. Magnetic resonance imaging has great potential for understanding better the underlying mechanisms of repetitive brain trauma. In this review, we provide an overview of advanced imaging techniques currently used to investigate brain anomalies. We also provide an overview of neuroimaging findings in those exposed to repetitive head impacts in the acute/subacute and chronic phase of injury and in more neurodegenerative phases of injury, as well as in military personnel exposed to repetitive head impacts. Finally, we discuss future directions for research that will likely lead to a better understanding of the underlying mechanisms separating those who recover from repetitive brain trauma vs. those who go on to develop CTE.

  14. FEMA Hazard Mitigation Assistance Severe Repetitive Loss (SRL) Data

    Data.gov (United States)

    Department of Homeland Security — This dataset contains closed and obligated projects funded under the following Hazard Mitigation Assistance (HMA) grant programs: Severe Repetitive Loss (SRL). The...

  15. Repetitive control of electrically driven robot manipulators

    Science.gov (United States)

    Fateh, Mohammad Mehdi; Ahsani Tehrani, Hojjat; Karbassi, Seyed Mehdi

    2013-04-01

    This article presents a novel robust discrete repetitive control of electrically driven robot manipulators for tracking of a periodic trajectory. We propose a novel model, which presents the highly non-linear dynamics of robot manipulator in the form of linear discrete-time time-varying system. Based on the proposed model, we develop a two-term control law. The first term is an ordinary time-optimal and minimum-norm (TOMN) control by employing parametric controllers to guarantee stability. The second term is a novel robust control to improve the control performance in the face of uncertainties. The robust control estimates and compensates uncertainties including the parametric uncertainty, unmodelled dynamics and external disturbances. Performance of the proposed method is compared with two discrete methods, namely the TOMN control and an adaptive iterative learning (AIL) control. Simulation results confirm superiority of the proposed method in terms of the convergence speed and precision.

  16. Studies of the uncanny: the repetition factor

    Directory of Open Access Journals (Sweden)

    Julia Teitelroit Martins

    2011-06-01

    Full Text Available Freud’s essay The Uncanny (Das Unheimliche offers many indications for the comprehension of an aesthetics of the uncanny which deserve to be explored. Nonetheless, a concept traverses it from beginning to end: the return – which enables its reading under the light of Beyond the pleasure principle, written along the same span of time. Emphasis is given to the uncanny in the sense of repetition of the different – a paradox in terms, like the strangely familiar uncanny. In order to test the validity of an aesthetic reading under this perspective, follows an analysis of the brief short story “A terceira margem do rio” (“The third margin of the river”, by Guimarães Rosa.

  17. Object color affects identification and repetition priming.

    Science.gov (United States)

    Uttl, Bob; Graf, Peter; Santacruz, Pilar

    2006-10-01

    We investigated the influence of color on the identification of both non-studied and studied objects. Participants studied black and white and color photos of common objects and memory was assessed with an identification test. Consistent with our meta-analysis of prior research, we found that objects were easier to identify from color than from black and white photos. We also found substantial priming in all conditions, and study-to-test changes in an object's color reduced the magnitude of priming. Color-specific priming effects were large for color-complex objects, but minimal for color-simple objects. The pattern and magnitude of priming effects was not influenced either by the extent to which an object always appears in the same color (i.e., whether a color is symptomatic of an object) or by the object's origin (natural versus fabricated). We discuss the implications of our findings for theoretical accounts of object perception and repetition priming.

  18. 米醋中蜡状芽孢杆菌DNA提取及其ERIC-PCR体系建立%Extraction of Bacillus cereus DNA in Vinegar and the Establishment of ERIC-PCR System

    Institute of Scientific and Technical Information of China (English)

    廖永红; 任文雅; 孙宝国; 徐瑾; 沈晗

    2011-01-01

    以蜡状芽孢杆菌(Bacillus cereus)为试验材料,比较细菌基因组DNA的提取方法.将ERIC-PCR应用于醋液分离菌的研究,对此反应体系的主要因素进行优化,最终建立了适合于此菌种的ERIC-PCR体系.采用改良的传统细菌基因组DNA提取方法,所提取的DNA质量较高,能够满足ERIC-PCR反应的需要.反应体系:25μL反应体积10×扩增缓冲液(含Mg2+)2.5 μL,20 pmol/μL ERIC-PCR引物E1 1μL,20 pmol/μL ERIC-PCR引物E2 1μL,DNA模板2μL,2.5 mmol/LdNTPs混合液2.0 μL,taq聚合酶1.1 μL,双蒸水补齐;PCR反应程序为:94℃变性3min,1个循环;94℃变性30 s,55℃退火40 s,72℃延伸1min,30个循环;72℃延伸4min.%Bacillus cereus was used as experimental materials. Hie method of genomic DNA extraction was studied in Bacillus and the conditions of ERIC-PCR. Finally, ERIC-PCR reaction system suitable for our lab was established. The results showed that high-grade genomic DNA which could meet the requirements of PCR reaction was obtained by the modified methods. The established ERIC-PCR reaction system was as follows: 10×Buffer (Mg2+) 2.5 μL, 20 pmol/μL primer E1 1 μL, 20 pmol/μL primer E2 1 μL, DNA template 2 μL, 2.5 mmol/L dNTPs 2 μL, Taq polymerase 1.1 μL, ddH2O 15.4 μL, 25 μL reaction volume. The reaction program of PCR was devised as follows: 3 min degeneration at 94 ℃ (one cycle), then 30s degeneration at 94 ℃, 40s annealing at 55 ℃, and 1 min extension at 72 ℃(30cycles), and then 4min final extension at 72 ℃.

  19. Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities.

    Science.gov (United States)

    Glauser, Daniel L; Seyffert, Michael; Strasser, Regina; Franchini, Marco; Laimbacher, Andrea S; Dresch, Christiane; de Oliveira, Anna Paula; Vogel, Rebecca; Büning, Hildegard; Salvetti, Anna; Ackermann, Mathias; Fraefel, Cornel

    2010-04-01

    Adeno-associated virus (AAV) has previously been shown to inhibit the replication of its helper virus herpes simplex virus type 1 (HSV-1), and the inhibitory activity has been attributed to the expression of the AAV Rep proteins. In the present study, we assessed the Rep activities required for inhibition of HSV-1 replication using a panel of wild-type and mutant Rep proteins lacking defined domains and activities. We found that the inhibition of HSV-1 replication required Rep DNA-binding and ATPase/helicase activities but not endonuclease activity. The Rep activities required for inhibition of HSV-1 replication precisely coincided with the activities that were responsible for induction of cellular DNA damage and apoptosis, suggesting that these three processes are closely linked. Notably, the presence of Rep induced the hyperphosphorylation of a DNA damage marker, replication protein A (RPA), which has been reported not to be normally hyperphosphorylated during HSV-1 infection and to be sequestered away from HSV-1 replication compartments during infection. Finally, we demonstrate that the execution of apoptosis is not required for inhibition of HSV-1 replication and that the hyperphosphorylation of RPA per se is not inhibitory for HSV-1 replication, suggesting that these two processes are not directly responsible for the inhibition of HSV-1 replication by Rep.

  20. Treatment with lenalidomide (Revlimid®), cyclophosphamide (Endoxan®) and prednisone (REP) in relapsed/refractory multiple myeloma patients: results of a single centre retrospective study.

    Science.gov (United States)

    Zelis, N; Devos, T; Dierickx, D; Janssens, A; Raddoux, J; Verhoef, G; Delforge, M

    2014-04-01

    Lenalidomide (Revlimid®) combined with intermittent dexamethasone (the RD regimen) is one of the current standards for treatment of patients with relapsed/refractory multiple myeloma (MM). However, since the disease in the majority of patients will become resistant to RD, or treatment with RD needs to be discontinued due to side effects, we evaluated the combination lenalidomide, low-dose oral cyclophosphamide, with prednisone (REP) in patients with relapsed/refractory MM previously exposed to RD. For this purpose, we performed a single centre retrospective study of the efficacy of REP in 19 patients with relapsed/refractory MM. Overall response rate (partial response or better) with REP was 68% compared with 83% with RD, but with a shorter time to response with the triplet REP. Time to progression after REP was 6 months. Overall the REP regimen was better tolerated compared to RD. We conclude that the REP regimen is an effective treatment regimen for patients with relapsed/refractory MM with good tolerance, warranting further exploration in prospective randomized trials.

  1. The golden ratio of gait harmony: repetitive proportions of repetitive gait phases.

    Science.gov (United States)

    Iosa, Marco; Fusco, Augusto; Marchetti, Fabio; Morone, Giovanni; Caltagirone, Carlo; Paolucci, Stefano; Peppe, Antonella

    2013-01-01

    In nature, many physical and biological systems have structures showing harmonic properties. Some of them were found related to the irrational number φ known as the golden ratio that has important symmetric and harmonic properties. In this study, the spatiotemporal gait parameters of 25 healthy subjects were analyzed using a stereophotogrammetric system with 25 retroreflective markers located on their skin. The proportions of gait phases were compared with φ, the value of which is about 1.6180. The ratio between the entire gait cycle and stance phase resulted in 1.620 ± 0.058, that between stance and the swing phase was 1.629 ± 0.173, and that between swing and the double support phase was 1.684 ± 0.357. All these ratios did not differ significantly from each other (F = 0.870, P = 0.422, repeated measure analysis of variance) or from φ (P = 0.670, 0.820, 0.422, resp., t-tests). The repetitive gait phases of physiological walking were found in turn in repetitive proportions with each other, revealing an intrinsic harmonic structure. Harmony could be the key for facilitating the control of repetitive walking. Harmony is a powerful unifying factor between seemingly disparate fields of nature, including human gait.

  2. The Golden Ratio of Gait Harmony: Repetitive Proportions of Repetitive Gait Phases

    Directory of Open Access Journals (Sweden)

    Marco Iosa

    2013-01-01

    Full Text Available In nature, many physical and biological systems have structures showing harmonic properties. Some of them were found related to the irrational number known as the golden ratio that has important symmetric and harmonic properties. In this study, the spatiotemporal gait parameters of 25 healthy subjects were analyzed using a stereophotogrammetric system with 25 retroreflective markers located on their skin. The proportions of gait phases were compared with , the value of which is about 1.6180. The ratio between the entire gait cycle and stance phase resulted in 1.620 ± 0.058, that between stance and the swing phase was 1.629 ± 0.173, and that between swing and the double support phase was 1.684 ± 0.357. All these ratios did not differ significantly from each other (, , repeated measure analysis of variance or from (, resp., t-tests. The repetitive gait phases of physiological walking were found in turn in repetitive proportions with each other, revealing an intrinsic harmonic structure. Harmony could be the key for facilitating the control of repetitive walking. Harmony is a powerful unifying factor between seemingly disparate fields of nature, including human gait.

  3. Iconicity in Discourse: The Case of Repetition in Japanese.

    Science.gov (United States)

    Ishikawa, Minako

    This analysis of repeated utterances in Japanese conversational discourse focuses on repetition as an expression of iconicity. In the analysis of a 30-minute conversation among 4 Japanese speakers, the iconic meanings expressed by both reduplication and conversational repetition are highlighted. The iconicity characteristic of conversational data…

  4. Evidence-Based Behavioral Interventions for Repetitive Behaviors in Autism

    Science.gov (United States)

    Boyd, Brian A.; McDonough, Stephen G.; Bodfish, James W.

    2012-01-01

    Restricted and repetitive behaviors (RRBs) are a core symptom of autism spectrum disorders (ASD). There has been an increased research emphasis on repetitive behaviors; however, this research primarily has focused on phenomenology and mechanisms. Thus, the knowledge base on interventions is lagging behind other areas of research. The literature…

  5. Visual attention to advertising : The impact of motivation and repetition

    NARCIS (Netherlands)

    Pieters, RGM; Rosbergen, E; Hartog, M; Corfman, KP; Lynch, JG

    1996-01-01

    Using eye-tracking data, we examine the impact of motivation and repetition on visual attention to advertisements differing in argument quality. Our analyses indicate that repetition leads to an overall decrease in the amount of attention. However, while at first high motivation subjects attend to t

  6. On the Functions of Lexical Repetition in English Texts

    Institute of Scientific and Technical Information of China (English)

    XIAO Fuliang

    2016-01-01

    Lexical repetition, as a cohesive device of an English text, can help make up a cohesive and coherent text. Therefore, in English textual learning, it is helpful for students to know about different patterns and functions of lexical repetition to improve their English level and ability.

  7. Covert radar and signals interception the secret career of Eric Ackermann

    CERN Document Server

    Hayson, David

    2014-01-01

    Of German stock dating back to 1530 in Saxony, Eric George Ackermann GM was born on the Isle of Wight in 1919 and became a leading figure in the world of signals and electronic intelligence. As a Junior Scientific Officer at the Telecommunication Research Establishment, Boscombe Down, with an honorary commission in the Royal Air Force, he made numerous flights over occupied territory searching for, monitoring and destroying Germany's Wuerzburg, Knickebein and X Band radar systems. Much of his research was passed to the highest levels of wartime government, and was highly prized, ensuring that

  8. The Happiness of an Abandoned Excellence. From Eric Weil's Moral Philosophy

    Directory of Open Access Journals (Sweden)

    Francis Guibal

    1998-12-01

    Full Text Available ls it possible to fit the ancient meaning of virtue into the modem parameters of liberty andautonomy? To answer that question we will lean on the thesis expounded by the Moral Philosophy of Eric Weil. This work, guided by Hegel, will help us measure the size of the antagonism between Aristotle and Kant, leading us through a meditation on historical action, and leading moral reflection to its vital and existential self-realization. Finally, we will assert the value and fertility, in absence of proofs, of this philosophical gesture.

  9. Gregory Bateson and Eric Voegelin – Silent Dialogues across the Human Sciences

    DEFF Research Database (Denmark)

    Thomassen, Bjørn

    2017-01-01

    This article argues that two important thinkers of the 20th century, Gregory Bateson (1904-1980) and Eric Voegelin (1901-1985), developed a set of ideas that are of importance to the history of the human sciences. The article also argues that their ideas display similarities that have not yet been...... discussed within the larger history of the human sciences. The aim of the article is to show how the diagnostic terms provided by Bateson and Voegelin complement each other toward an understanding of underlying tensions and social pathologies of contemporary civilization....

  10. The Happiness of an Abandoned Excellence. From Eric Weil's Moral Philosophy

    OpenAIRE

    Francis Guibal

    1998-01-01

    ls it possible to fit the ancient meaning of virtue into the modem parameters of liberty andautonomy? To answer that question we will lean on the thesis expounded by the Moral Philosophy of Eric Weil. This work, guided by Hegel, will help us measure the size of the antagonism between Aristotle and Kant, leading us through a meditation on historical action, and leading moral reflection to its vital and existential self-realization. Finally, we will assert the value and fertility, in absence of...

  11. The Happiness of an Abandoned Excellence. From Eric Weil's Moral Philosophy

    OpenAIRE

    Guibal, Francis

    2013-01-01

    ls it possible to fit the ancient meaning of virtue into the modem parameters of liberty andautonomy? To answer that question we will lean on the thesis expounded by the Moral Philosophy of Eric Weil. This work, guided by Hegel, will help us measure the size of the antagonism between Aristotle and Kant, leading us through a meditation on historical action, and leading moral reflection to its vital and existential self-realization. Finally, we will assert the value and fertility, in absence of...

  12. Repetitive DNA Sequences in Wheat and Its Relatives

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xue-yong; LI Da-yong

    2001-01-01

    Repetitive DNA sequences form a large portion of eukaryote genomes. Using wheat ( Triticum )as a model, the classification, features and functions of repetitive DNA sequences in the Tritieeae grass tribe is reviewed as well as the role of these sequences in genome differentiation, control and regulation of homologous chromosome synapsis and pairing. Transposable elements, as an important portion of dispersed repetitives,may play an essential role in gene mutation of the host. Dynamic models for change of copy number and sequences of the repetitive family are also presented after the models of Charlesworth et al. Application of repetitive DNA sequences in the study of evolution, chromosome fingerprinting and marker assisted gene transfer and breeding are described by taking wheat as an example.

  13. Nonword Repetition and Speech Motor Control in Children

    Directory of Open Access Journals (Sweden)

    Christina Reuterskiöld

    2015-01-01

    Full Text Available This study examined how familiarity of word structures influenced articulatory control in children and adolescents during repetition of real words (RWs and nonwords (NWs. A passive reflective marker system was used to track articulator movement. Measures of accuracy were obtained during repetition of RWs and NWs, and kinematic analysis of movement duration and variability was conducted. Participants showed greater consonant and vowel accuracy during RW than NW repetition. Jaw movement duration was longer in NWs compared to RWs across age groups, and younger children produced utterances with longer jaw movement duration compared to older children. Jaw movement variability was consistently greater during repetition of NWs than RWs in both groups of participants. The results indicate that increases in phonological short-term memory demands affect articulator movement. This effect is most pronounced in younger children. A range of skills may develop during childhood, which supports NW repetition skills.

  14. Soliton repetition rate in a silicon-nitride microresonator

    CERN Document Server

    Bao, Chengying; Wang, Cong; Jaramillo-Villegas, Jose A; Leaird, Daniel E; Qi, Minghao; Weiner, Andrew M

    2016-01-01

    The repetition rate of a Kerr comb comprising a single soliton in an anomalous dispersion silicon nitride microcavity is measured as a function of pump frequency tuning. The contributions from the Raman soliton self-frequency shift (SSFS) and from thermal effects are evaluated both experimentally and theoretically; the SSFS is found to dominate the changes in repetition rate. The relationship between the changes in repetition rate and pump frequency detuning is found to be independent of the nonlinearity coefficient and dispersion of the cavity. Modeling of the repetition rate change by using the generalized Lugiato-Lefever equation is discussed; the Kerr shock is found to have only a minor effect on repetition rate for cavity solitons with duration down to ~50 fs.

  15. Transgenerational effects of environmental enrichment on repetitive motor behavior development.

    Science.gov (United States)

    Bechard, Allison R; Lewis, Mark H

    2016-07-01

    The favorable consequences of environmental enrichment (EE) on brain and behavior development are well documented. Much less is known, however, about transgenerational benefits of EE on non-enriched offspring. We explored whether transgenerational effects of EE might extend to the development of repetitive motor behaviors in deer mice. Repetitive motor behaviors are invariant patterns of movement that, across species, can be reduced by EE. We found that EE not only attenuated the development of repetitive behavior in dams, but also in their non-enriched offspring. Moreover, maternal behavior did not seem to mediate the transgenerational effect we found, although repetitive behavior was affected by reproductive experience. These data support a beneficial transgenerational effect of EE on repetitive behavior development and suggest a novel benefit of reproductive experience.

  16. Soliton repetition rate in a silicon-nitride microresonator.

    Science.gov (United States)

    Bao, Chengying; Xuan, Yi; Wang, Cong; Jaramillo-Villegas, Jose A; Leaird, Daniel E; Qi, Minghao; Weiner, Andrew M

    2017-02-15

    The repetition rate of a Kerr comb composed of a single soliton in an anomalous group velocity dispersion silicon-nitride microcavity is measured as a function of pump frequency. By comparing operation in the soliton and non-soliton states, the contributions from the Raman soliton self-frequency shift (SSFS) and the thermal effects are evaluated; the SSFS is found to dominate the changes in the repetition rate, similar to silica cavities. The relationship between the changes in the repetition rate and the pump frequency detuning is found to be independent of the nonlinearity coefficient and dispersion of the cavity. Modeling of the repetition rate change by using the generalized Lugiato-Lefever equation is discussed; the Kerr shock is found to have only a minor effect on repetition rate for cavity solitons with duration down to ∼50  fs.

  17. Self-controlled KR schedules: does repetition order matter?

    Science.gov (United States)

    Patterson, Jae T; Carter, Michael J; Hansen, Steve

    2013-08-01

    The impact of an experimenter-defined repetition schedule on the utility of a self-controlled KR context during motor skill acquisition was examined. Participants were required to learn three novel spatial-temporal tasks in either a random or blocked repetition schedule with or without the opportunity to control their KR. Results from the retention period showed that participants provided control over their KR schedule in a random repetition schedule demonstrated superior learning. However, performance measures from the transfer test showed that, independent of repetition schedule, learners provided the opportunity to control their KR schedule demonstrated superior transfer performance compared to their yoked counterparts. The dissociated impact of repetition schedule and self-controlled KR schedules on retention and transfer is discussed.

  18. Impaired speech repetition and left parietal lobe damage.

    Science.gov (United States)

    Fridriksson, Julius; Kjartansson, Olafur; Morgan, Paul S; Hjaltason, Haukur; Magnusdottir, Sigridur; Bonilha, Leonardo; Rorden, Christopher

    2010-08-18

    Patients with left hemisphere damage and concomitant aphasia usually have difficulty repeating others' speech. Although impaired speech repetition, the primary symptom of conduction aphasia, has been associated with involvement of the left arcuate fasciculus, its specific lesion correlate remains elusive. This research examined speech repetition among 45 stroke patients who underwent aphasia testing and MRI examination. Based on lesion-behavior mapping, the primary structural damage most closely associated with impaired speech repetition was found in the posterior portion of the left arcuate fasciculus. However, perfusion-weighted MRI revealed that tissue dysfunction, in the form of either frank damage or hypoperfusion, to the left inferior parietal lobe, rather than the underlying white matter, was associated with impaired speech repetition. This latter result suggests that integrity of the left inferior parietal lobe is important for speech repetition and, as importantly, highlights the importance of examining cerebral perfusion for the purpose of lesion-behavior mapping in acute stroke.

  19. Repetition and Emotive Communication in Music Versus Speech

    Directory of Open Access Journals (Sweden)

    Elizabeth Hellmuth eMargulis

    2013-04-01

    Full Text Available Music and speech are often placed alongside one another as comparative cases. Their relative overlaps and disassociations have been well explored (e.g. Patel, 2010. But one key attribute distinguishing these two domains has often been overlooked: the greater preponderance of repetition in music in comparison to speech. Recent fMRI studies have shown that familiarity – achieved through repetition – is a critical component of emotional engagement with music (Pereira et al., 2011. If repetition is fundamental to emotional responses to music, and repetition is a key distinguisher between the domains of music and speech, then close examination of the phenomenon of repetition might help clarify the ways that music elicits emotion differently than speech.

  20. Physical Characteristics Underpinning Repetitive Lunging in Fencing.

    Science.gov (United States)

    Turner, Anthony N; Marshall, Geoff; Phillips, James; Noto, Angelo; Buttigieg, Conor; Chavda, Shyam; Downing, William; Atlay, Nathan; Dimitriou, Lygeri; Kilduff, Laim

    2016-11-01

    Turner, AN, Marshall, G, Phillips, J, Noto, A, Buttigieg, C, Chavda, S, Downing, W, Atlay, N, Dimitriou, L, and Kilduff, L. Physical characteristics underpinning repetitive lunging in fencing. J Strength Cond Res 30(11): 3134-3139, 2016-Given the repetitive demand to execute lunging and changes in direction within fencing, the ability to sustain these at maximal capacity is fundamental to performance. The aim of this study was threefold. First, to provide normative values for this variable referred to as repeat lunge ability (RLA) and second to identify the physical characteristics that underpin it. Third, was to establish if a cause and effect relationship existed by training the associated characteristics. Assessment of lower-body power, reactive strength, speed, change of direction speed (CODS), and a sport-specific RLA were conducted on senior and junior elite male fencers (n = 36). Fencers were on average (±SD) 18.9 ± 3.2 years of age, 174.35 ± 10.42 cm tall, 70.67 ± 7.35 kg in mass, and 8.5 ± 4.2 years fencing experience. The RLA test had average work times of 16.03 ± 1.40 seconds and demonstrated "large" to "very large" associations with all tested variables, but in particular CODS (r = 0.70) and standing broad jump (SBJ; r = -0.68). Through linear regression analysis, these also provided a 2-predictor model accounting for 61% of the common variance associated with RLA. A cause and effect relationship with SBJ and CODS was confirmed by the training group, where RLA performance in these fencers improved from 15.80 ± 1.07 to 14.90 ± 0.86 seconds, with the magnitude of change reported as "moderate" (effect size (ES) = 0.93). Concurrent improvements were also noted in both SBJ (216.86 ± 17.15 vs. 221.71 ± 17.59 cm) and CODS (4.44 ± 0.29 vs. 4.31 ± 0.09 seconds) and while differences were only significant in SBJ, magnitudes of change were classed as "small" (ES = 0.28) and "moderate" (ES = 0.61), respectively. In conclusion, to improve RLA strength

  1. El montaje de la transición argentina. Un análisis de los films La República perdida, La República perdida II y Evita, quien quiera oír que oiga

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    Paola Judith Margulis

    2012-01-01

    Full Text Available El presente artículo se propone reconstruir una zona de la historia del documental argentino a partir del abordaje de tres films que tuvieron un alto impacto en Argentina hacia la transición democrática: La República perdida (Miguel Pérez, 1983, su secuela La República perdida II (Miguel Pérez, 1986 y Evita, quien quiera oír que oiga (Eduardo Mignogna, 1984. Dada la importancia de estos films -tanto en términos políticos como de impacto en el público-, intentaremos situarlos en contexto y analizar algunos aspectos inherentes a su lógica fílmica.

  2. Lecturas de un sueño. (Sobre la República literaria de Saavedra Fajardo

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    Rodríguez, Juan Carlos

    2013-12-01

    Full Text Available The author of the República Literaria, a diplomatic voyager from the 17th Century, dreams of a city of paper: to read a city as if it were a book and to read a book as if it were a journey throughout literary history. Is it possible? On this journey across all of written culture that Saavedra describes, where the inhabitants of the city are authors of ‘literary’ texts and the arts live together with the sciences and the muses, the author analyses with subtle irony the present and the tradition of everything that surrounds the production of all that is written. With the classic resource of the ‘Dream’ he shows an incredibly clear panorama where we can recognize the beginnings of capitalism, in which books have gone from being considered repositories of wisdom and common sense, to becoming mere marketplace objects.El autor de la República Literaria, un diplomático viajero del siglo XVII, sueña una ciudad de papel: leer una ciudad como si fuera un libro y leer un libro como si fuera un viaje a través de la historia literaria. ¿Es posible? En este recorrido por toda la cultura escrita que traza Saavedra, donde los habitantes de la ciudad son los autores de los textos «literarios» y las artes conviven con las ciencias y las musas, el autor desmenuza con finísima ironía la actualidad y la tradición de todo lo que rodea a la producción de lo escrito. Con el clásico recurso del «Sueño» muestra un panorama increíblemente nítido donde podemos reconocer los inicios del capitalismo en el que los libros han pasado de ser considerados como un depósito de sabiduría y cordura a tener carácter de mero objeto de mercado.

  3. Generation of low-timing-jitter femtosecond pulse trains with 2 GHz repetition rate via external repetition rate multiplication.

    Science.gov (United States)

    Chen, Jian; Sickler, Jason W; Fendel, Peter; Ippen, Erich P; Kärtner, Franz X; Wilken, Tobias; Holzwarth, Ronald; Hänsch, Theodor W

    2008-05-01

    Generation of low-timing-jitter 150 fs pulse trains at 1560 nm with 2 GHz repetition rate is demonstrated by locking a 200 MHz fundamental polarization additive-pulse mode-locked erbium fiber laser to high-finesse external Fabry-Perot cavities. The timing jitter and relative intensity noise of the repetition-rate multiplied pulse train are investigated.

  4. [Rehabilitation Using Repetitive Transcranial Magnetic Stimulation].

    Science.gov (United States)

    Takeuchi, Naoyuki; Izumi, Shin-Ichi

    2017-03-01

    Various novel stroke rehabilitative methods have been developed based on findings in basic science and clinical research. Recently, many reports have shown that repetitive transcranial magnetic stimulation (rTMS) improves function in stroke patients by altering the excitability of the human cortex. The interhemispheric competition model proposes that deficits in stroke patients are due to reduced output from the affected hemisphere and excessive interhemispheric inhibition from the unaffected hemisphere to the affected hemisphere. The interhemispheric competition model indicates that improvement in deficits can be achieved either by increasing the excitability of the affected hemisphere using excitatory rTMS or by decreasing the excitability of the unaffected hemisphere using inhibitory rTMS. Recovery after stroke is related to neural plasticity, which involves developing new neural connections, acquiring new functions, and compensating for impairments. Artificially modulating the neural network by rTMS may induce a more suitable environment for use-dependent plasticity and also may interfere with maladaptive neural activation, which weakens function and limits recovery. There is potential, therefore, for rTMS to be used as an adjuvant therapy for developed neurorehabilitation techniques in stroke patients.

  5. SI Engine with repetitive NS spark plug

    Science.gov (United States)

    Pancheshniy, Sergey; Nikipelov, Andrey; Anokhin, Eugeny; Starikovskiy, Andrey; Laplase Team; Mipt Team; Pu Team

    2013-09-01

    Now de-facto the only technology for fuel-air mixtures ignition in IC engines exists. It is a spark discharge of millisecond duration in a short discharge gap. The reason for such a small variety of methods of ignition initiation is very specific conditions of the engine operation. First, it is very high-pressure of fuel-air mixture - from 5-7 atmospheres in old-type engines and up to 40-50 atmospheres on the operating mode of HCCI. Second, it is a very wide range of variation of the oxidizer/fuel ratio in the mixture - from almost stoichiometric (0.8-0.9) at full load to very lean (φ = 0.3-0.5) mixtures at idle and/or economical cruising mode. Third, the high velocity of the gas in the combustion chamber (up to 30-50 m/s) resulting in a rapid compression of swirling inlet flow. The paper presents the results of tests of distributed spark ignition system powered by repetitive pulse nanosecond discharge. Dynamic pressure measurements show the increased pressure and frequency stability for nanosecond excitation in comparison with the standard spark plug. Excitation by single nanosecond high-voltage pulse and short train of pulses was examined. In all regimes the nanosecond pulsed excitation demonstrate a better performance.

  6. Repetitive transcranial magnetic stimulation and drug addiction.

    Science.gov (United States)

    Barr, Mera S; Farzan, Faranak; Wing, Victoria C; George, Tony P; Fitzgerald, Paul B; Daskalakis, Zafiris J

    2011-10-01

    Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation technique that is now being tested for its ability to treat addiction. This review discusses current research approaches and results of studies which measured the therapeutic use of rTMS to treat tobacco, alcohol and illicit drug addiction. The research in this area is limited and therefore all studies evaluating the therapeutic use of rTMS in tobacco, alcohol or illicit drug addiction were retained including case studies through NCBI PubMed ( http://www.ncbi.nlm.nih.gov ) and manual searches. A total of eight studies were identified that examined the ability of rTMS to treat tobacco, alcohol and cocaine addiction. The results of this review indicate that rTMS is effective in reducing the level of cravings for smoking, alcohol, and cocaine when applied at high frequencies to the dorsolateral prefrontal cortex (DLPFC). Furthermore, these studies suggest that repeated sessions of high frequency rTMS over the DLPFC may be most effective in reducing the level of smoking and alcohol consumption. Although work in this area is limited, this review indicates that rTMS is a promising modality for treating drug addiction.

  7. Development of a repetitive compact torus injector

    Science.gov (United States)

    Onchi, Takumi; McColl, David; Dreval, Mykola; Rohollahi, Akbar; Xiao, Chijin; Hirose, Akira; Zushi, Hideki

    2013-10-01

    A system for Repetitive Compact Torus Injection (RCTI) has been developed at the University of Saskatchewan. CTI is a promising fuelling technology to directly fuel the core region of tokamak reactors. In addition to fuelling, CTI has also the potential for (a) optimization of density profile and thus bootstrap current and (b) momentum injection. For steady-state reactor operation, RCTI is necessary. The approach to RCTI is to charge a storage capacitor bank with a large capacitance and quickly charge the CT capacitor bank through a stack of integrated-gate bipolar transistors (IGBTs). When the CT bank is fully charged, the IGBT stack will be turned off to isolate banks, and CT formation/acceleration sequence will start. After formation of each CT, the fast bank will be replenished and a new CT will be formed and accelerated. Circuits for the formation and the acceleration in University of Saskatchewan CT Injector (USCTI) have been modified. Three CT shots at 10 Hz or eight shots at 1.7 Hz have been achieved. This work has been sponsored by the CRC and NSERC, Canada.

  8. Library and Information Services for Literacy. A Select ERIC Bibliography Prepared on a Theme of the 1991 White House Conference on Library and Information Services. ERIC/IR Mini-Bib.

    Science.gov (United States)

    Preston, Nancy R., Comp.

    Documents and journal articles featured in this nine-item annotated bibliography concern the role of library and information services in developing and expanding literacy at all levels in an increasingly information-oriented society. Citations were selected through a computer search of the Educational Resources Information Center (ERIC) database.…

  9. Pleonexía: el centro ausente de La República de Platón

    OpenAIRE

    Carrasco, Nemrod

    2008-01-01

    La pleonexía es una categoría platónica más complicada de lo que puede parecer. Tradicionalmente asociada a las posiciones de Calicles y Trasímaco, lo primero que nos indica La república es que la pleonexía no es únicamente el motor constitutivo de toda ciudad, sino el punto de referencia decisivo de la concepción platónica de la justicia. Es ahí donde radica la paradoja del tránsito de la ciudad sana de Sócrates a la ciudad enferma de Glaucón: lejos de rep...

  10. Liberalismo, iluminismo y romanticismo: el problema de la libertad en dos novelas de la República Restaurada

    Directory of Open Access Journals (Sweden)

    Verónica Hernández Landa Valencia

    2016-07-01

    Full Text Available El artículo propone una reflexión en torno a la forma como influyen tres corrientes de pensamiento, el liberalismo, el iluminismo y el romanticismo, en la representación del problema de la libertad en dos novelas históricas publicadas en la República Restaurada: Monja y casada, virgen y mártir, de Vicente Riva Palacio, y El pecado del siglo, de José Tomás de Cuéllar. Se busca mostrar que dichas corrientes se entrecruzan en un solo texto, que ello contribuye a la conformación de un concepto de libertad restringida y que la forma específica que adquiere ese concepto se explica a partir de la postura ideológica y política de los autores en el contexto de la República Restaurada.

  11. 150 años despues: Constitución de la república de Tunja

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    José Hurtado García

    1961-12-01

    Full Text Available En Tunja perduran abiertos los corazones y los zaguanes. La Villa noble de Suárez Rendón, embelesada en el canto de la Madre Castillo y de don Juan de Castellanos, amaneció en la República con el sacrificio de sus hijos y con sus ideas proceras. Precisamente en este mes de diciembre de 1961 -el nueve- se cumplen los ciento cincuenta años de la expedición de su Constitución, cuyo título definido reza: " Constitución de la República de Tunja sancionada en plena Asamblea de los Representantes de toda la Provincia, en sesiones continuas desde el 21 de noviembre hasta el 9 de diciembre de 1811. Año Segundo de la Transformación Política del Nuevo Reyno de Granada".

  12. A “Primeira República”: os preceitos liberais e a práxis oligárquica

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    Flávia Liziane Gonzáles Bandeira

    2009-04-01

    Full Text Available O presente artigo visa a analisar o processo de mudança de regime político no Brasil, no final do século XIX – o advento da República. As fontes utilizadas da análise são um fragmento da primeira Constituição republicana e os programas dos partidos Republicano Federal e Republicano Conservador. Visou-se a buscar os limites entre o discurso encontrado nesses documentos e a prática política realizada no Brasil da República Velha. Para melhor extrair o teor do documento foi utilizada a técnica metodológica de análise de conteúdo quantitativa, nomeando unidades referenciais mais incidentes nos respectivos textos, seguida da análise qualitativa aplicada a estas.

  13. Balancing Biography and Institutional History: Eric Worrell’s Australian Reptile Park

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    Nancy Cushing

    2009-11-01

    Full Text Available When a young naturalist opened his new wildlife park at Wyoming on the NSW Central Coast in the late 1950s, he gave it his own name: Eric Worrell’s Australian Reptile Park. Through the park, Worrell made a significant contribution to environmental education, the development of knowledge of captive animal care and display and the provision of antivenoms for the bites of a range of dangerous creatures. More than this, it was the geographic and emotional centre of Worrell’s world: the fulfilment of a childhood dream, a home for his family and a site for forming new personal and professional relationships. In preparation for the jubilee of the park, its history is being written by two academics from the University of Newcastle. An attractive means of creating the necessary narrative structure and human interest to ensure the wide appeal of this history is to follow Worrell’s lead and place his life at the centre of this institutional history. This is the direction suggested by the written sources on the park and it is accentuated by many of our oral informants who organise their memories of the park around Worrell. To what extent can an institutional history be a biography of the person at the heart of that institution? Is it possible to disentangle the life from the institution? This article offers some preliminary answers to these questions through a case study of the writing of a history of Eric Worrell’s Australian Reptile Park.

  14. O sistema oligárquico representativo da Primeira República

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    Wanderley Guilherme dos Santos

    2013-03-01

    Full Text Available Entre les absolutismes des XVIIe et XVIIIe siècles, d'une part, et les variantes démocratiques repérées après la Seconde Guerre Mondiale, d'autre part, demeure un brouillard conceptuel gênant la compréhension de l'histoire politico-institutionnelle de cette période. L'oligarchie, traitée soit comme germe de démocratie soit comme vestige autocratique, reste une période soumise à la subjectivité. On présente un modèle analytique différent de ce que serait un système oligarchique, à la validité trans-locale, en cherchant à montrer la façon dont l'histoire du Second Empire et de la Première République apparaitrait au moyen d'une approche préalable utilisant le modèle général.

  15. Desterrando formas poéticas en la República de Platón.

    Directory of Open Access Journals (Sweden)

    Sergio Ariza.

    2009-12-01

    Full Text Available Este artículo examina un aspecto seminal, pero sorprendente en la crítica de Platón de la poesía: Platón destierra a los contenidos, no sólo particular, y los autores, pero las formas poéticas también ellas mismas, en concreto la forma mimética. El análisis muestra que la principal razón para desterrar tales reside en el hecho de que Platón descubrió que la forma como la forma (con independencia de su contenido tiene un carácter autónomo y se rige por criterios estéticos y no moral. Estos criterios estéticos son incompatibles con la ideología que apoya la polis ideal. Esto pone de relieve el mérito del análisis de Platón de que no está en la evaluación de la actividad poética, sino en la identificación de las puramente estéticas y sus consecuencias sobre el programa político de la República

  16. Leptospirosis porcina: prevalencia serológica en establecimientos productores de la República Argentina

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    Jessica Petrakovsky M.

    2013-03-01

    Full Text Available Objetivo. Detectar la presencia de anticuerpos contra Leptospira sp en cerdos que no presentaban sintomatología clínica, pertenecientes a establecimientos de producción porcina ubicados en todo el territorio de la República Argentina. Materiales y métodos. La asignación de predios y muestras se realizó en forma proporcional a los registrados en cada provincia a lo largo de todo el país. En total se estudiaron 1.524 predios, de los cuales se tomaron 19.872 muestras de suero y en la primera etapa se procesaron 3.631 de los mismos. Este estudio toma en consideración zonas identificadas por provincia, municipio y predios. Los sueros fueron analizados por la técnica de MAT (prueba de microaglutinación. Resultados. El 30% de las muestras resultaron positivas a la prueba tamiz empleada (primera etapa del MAT. La mayoría presentó coaglutininas a dos o a tres serovariedades. Al realizar la titulación de los sueros (segunda etapa del MAT, el porcentaje de positividad se mantuvo, siendo los serovares de mayor prevalencia Castellonis e Icterohaemorrhagiae. El 90% de las muestras presentó un título final de 1:200. Conclusiones. La existencia de sueros positivos en todas las provincias indica el contacto de los animales con la leptospira a lo largo de todo el país.

  17. Representações cômicas da República no contexto do Getulismo

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    Elio Chaves Flores

    2001-01-01

    Full Text Available Neste artigo analiso as representações cômicas e discursivas da República e a trajetória política de Getúlio Vargas, a partir de uma narrativa que se assenta no método regressivo. Para isso, trabalhei com uma série de caricaturas publicadas em jornais e revistas na duração entre 1930 e 1954, confrontando a intertextualidade dessas imagens com alguns discursos satíricos e irônicos produzidos por memorialistas. Procuro demonstrar que há semelhança entre a inversão humorística e a textualidade da memória, quando inventam a respeito de seu tempo e dos outros imagens verossímeis.This article analyses the comic and discursive representations of the Brazilian Republic and Getúlio Vargas’ political career, starting from a narrative constructed in the regressive method. For that, I worked with a series of cartoons published in newspapers and magazines in the span of time between 1954 and 1930, matching the intertextuality of these images with some of the satiric and ironic speeches produced by witnesses. It is demonstrated there is likeness between the comic inversion and the textuality of the memory, when they invent concerning their time and other ones, the verisimilitude of the images.

  18. A loucura e a república no Brasil: a influência das teorias raciais

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    Audrey Rossi Weyler

    2006-03-01

    Full Text Available O presente artigo tem como objetivo discutir as modificações nas formas de compreensão e de enfrentamento da loucura na passagem do Império para a República, levando-se em conta a influência das teorias raciais importadas da Europa a partir de 1870. A adoção desses modelos teóricos cumpriu uma série de funções sociais e políticas referente a um projeto de nação. Neste contexto, as manifestações da loucura passaram a ser associadas à doença, à degeneração e ao risco e sofreram variadas medidas de opressão justificadas "cientificamente". Até hoje, as interpretações científicas do problema da "doença mental" continuam localizando a enfermidade no paciente. Ao reduzir a doença e a sua causa a uma problemática exclusivamente pessoal, sem que se considere o contexto socioeconômico e político da sua emergência, estas explicações configuram-se como ideologia.

  19. Les revistes bilingües a la Tortosa de la II República

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    M. Carme Gombau Domingo

    2016-06-01

    Full Text Available En l’àmbit de la premsa tortosina en català del primer terç del segle xx es donen dues actituds lingüístiques: la fabriana i la tortosinista. La primera, partidària del model de llengua de l’IEC, era minoritària als anys vint però es generalitza a partir dels anys trenta (La Veu de Tortosa, La Veu Comarcal, Ara, Acció, Vida Tortosina i Lluita; mentre que el tortosinisme, que es correspon amb una ideologia cada cop més localista i que eleva el «parlar tortosí» a la categoria de llengua, queda reduït durant la II República a tres revistes bilingües: La Zuda, Germanor i La Santa Cinta. There were two linguistic actitudes in the tortosine press of the first third in the 20th century: the fabriana amb the tortosinista. The fabriana, in favour of the IEC linguistic model, was in minority during the 20’s, but it became widespread from the 30’s (La Veu de Tortosa, La Veu Comarcal, Ara, Acció, Vida Tortosina i Lluita. The second, the tortosinista, had a local ideology and compared the tortosinisme talk as a complet language, but it was reduced during the ii Republic to three magazines: La Zuda, Germanor i La Santa Cinta.

  20. Interspecies extrapolation based on the RepDose database--a probabilistic approach.

    Science.gov (United States)

    Escher, Sylvia E; Batke, Monika; Hoffmann-Doerr, Simone; Messinger, Horst; Mangelsdorf, Inge

    2013-04-12

    Repeated dose toxicity studies from the RepDose database (DB) were used to determine interspecies differences for rats and mice. NOEL (no observed effect level) ratios based on systemic effects were investigated for three different types of exposure: inhalation, oral food/drinking water and oral gavage. Furthermore, NOEL ratios for local effects in inhalation studies were evaluated. On the basis of the NOEL ratio distributions, interspecies assessment factors (AF) are evaluated. All data sets were best described by a lognormal distribution. No difference was seen between inhalation and oral exposure for systemic effects. Rats and mice were on average equally sensitive at equipotent doses with geometric mean (GM) values of 1 and geometric standard deviation (GSD) values ranging from 2.30 to 3.08. The local AF based on inhalation exposure resulted in a similar distribution with GM values of 1 and GSD values between 2.53 and 2.70. Our analysis confirms former analyses on interspecies differences, including also dog and human data. Furthermore it supports the principle of allometric scaling according to caloric demand in the case that body doses are applied. In conclusion, an interspecies distribution animal/human with a GM equal to allometric scaling and a GSD of 2.5 was derived.

  1. República federal y patronato: el ascenso y descalabro de un proyecto

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    Brian Connaughton

    2010-06-01

    Full Text Available Este artículo aborda el patronato durante la primera república federal (1824-1834 desde la perspectiva de los debates que lo rodearon y dificultaron una solución por la cual el gobierno mexicano ejerciera los derechos de patrono de la Iglesia. La polémica sobre el patronato tuvo aspectos eclesiológicos, políticos y diplomáticos. Permite ver que México estaba inserto en una compleja dinámica nacional e internacional que acabaría frustrando la firma de un concordato.This article examines the trust established during the first federal republic (1824-1834 from the perspective of the debates that surrounded it and prevented reaching a solution whereby the Mexican government would act as owner of the Church. The polemic over the trust had ecclesiastic, political and diplomatic aspects. It showed that Mexico was inserted in a complex national and international dynamic that would end up preventing the signing of a concordat.

  2. Sistema de salud de República Dominicana The health system of Dominican Republic

    Directory of Open Access Journals (Sweden)

    Magdalena Rathe

    2011-01-01

    Full Text Available En este trabajo se describen las condiciones de salud de la República Dominicana y, con mayor detalle, el sistema de salud dominicano, incluyendo su estructura y cobertura, sus fuentes de financiamiento, el gasto en salud, los recursos físicos, materiales y humanos de los que dispone, las tareas de rectoría que desarrolla el Ministerio de Salud Pública y la generación de información. También se discuten la participación de los usuarios en la operación y evaluación del sistema de salud y las más recientes innovaciones implementadas, dentro de las que destacan la nueva Ley General de Salud, la nueva Ley de Seguridad Social y el Plan Decenal de Salud.This paper describes the health conditions in Dominican Republic and the characteristics of the Dominican health system, including its structure and coverage, its financial sources, the health expenditure, the physical, material and human resources available, the stewardship functions developed by the Ministry of Public Health and the generation of health information. The participation of health care users in the operation and evaluation of the system and the most recent policy innovations, including the new General Health Law, the new Social Security Law and the Decennial Health Plan are also discussed.

  3. Mapa educativo nacional. Una experiencia en la República Argentina

    Directory of Open Access Journals (Sweden)

    Horacio Rodolfo Bozzano

    2006-01-01

    Full Text Available El Proyecto Mapa Educativo Nacional (MEN es un SIG generado a partir del desarrollo e integración de 24 Mapas Educativos Provinciales sobre la base de un modelo conceptual general, con una visión dinámica de los 49.000 establecimientos educativos, así como de su contexto territorial. Los objetivos del trabajo son: a sistematizar los aportes al Proyecto MEN desde la teoría cartográfica, reconociendo instancias de concepción, construcción y comunicación de los mapas que lo integran, así como vinculaciones entre territorio, espacio y espacialidades emergentes de la concreción de la tarea; y b identificar en qué medida la concreción del Proyecto MEN en la República Argentina contribuye a fortalecer un enfoque dialéctico conocimiento-realidad sobre el territorio y la gestión aplicado a un objeto de estudio e intervención concreto de desarrollo institucional, con el propósito de promover un círculo virtuoso de transformación entre sujetos de cambio y agentes de desarrollo.

  4. La Historia de la Psicología en la República Dominicana

    Directory of Open Access Journals (Sweden)

    Josefina Zaite

    2013-01-01

    Full Text Available Este trabajo revisa el desarrollo de la psicología académica y profesional en la República Dominicana. Se da relevancia a los acontecimientos socio-históricos que sirvieron de contexto para el surgimiento y el avance de la psicología dominicana. Los aportes de los y las profesionales que contribuyen al impulso de los estudios y el ejercicio profesional de la psicología en nuestro país, son señalados y reconocidos. En el trabajo se aborda un análisis de los ámbitos de realización; así como de los logros y de las dificultades, en el avance tanto de la psicología académica, como del ejercicio profesional en la psicología dominicana. Finalmente, se consideran las perspectivas actuales de la psicología dominicana, para su desarrollo, su integración y proyección en el conjunto de la psicología en el Caribe y en Latinoamérica.

  5. Dos nuevas publicaciones de la Biblioteca Luis Ángel Arango del Banco de la República

    Directory of Open Access Journals (Sweden)

    Boletín Cultural y Bibliográfico Banco de la República

    1961-11-01

    Full Text Available La Biblioteca Luis-Ángel Arango, del Banco de la República, acaba de dar a la publicidad dos importantes trabajos que serán inapreciable ayuda para los investigadores de las letras colombianas, como son el volumen del Catálogo de su espléndida colección y Papeletas Bibliográficas para el estudio de la historia colombiana.

  6. Molecular Dynamics of Rab7::REP1::GGTase-II Ternary Complex and Identification of Their Putative Drug Binding Sites.

    Science.gov (United States)

    Sindhu, Meenakshi; Saini, Vandana; Piplani, Sakshi; Kumar, A

    2013-01-01

    The structure-function correlation of membrane proteins have been a difficult task, particularly in context to transient protein complexes. The molecular simulation of ternary complex of Rab7::REP1::GGTase-II was carried out to understand the basic structural events occurring during the prenylation event of Rab proteins, using the software YASARA. The study suggested that the C-terminus of Rab7 has to be in completely extended conformation during prenylation to reach the active site of RabGGTase-II. Also, attempt was made to find putative drug binding sites on the ternary complex of Rab7::REP1::GGTase-II using Q-SiteFinder programme. The comprehensive consensus probe generated by the program revealed a total of 10 major pockets as putative drug binding sites on Rab7::REP:: GGTase-II ternary complex. These pockets were found on REP protein and GGTase protein subunits. The Rab7 was found to be devoid of any putative drug binding sites in the ternary complex. The phylogenetic analysis of 60 Rab proteins of human was carried out using PHYLIP and study indicated the close phylogenetic relationship between Rab7 and Rab9 proteins of human and hence with further in silico study, the present observations can be extrapolated to Rab9 proteins. The study paves a good platform for further experimental verifications of the findings and other in silico studies like identifying the potential drug targets by searching the putative drug binding sites, generating pharmacophoric pattern, searching or constructing suitable ligand and docking studies.

  7. CARACTERIZACIÓN MOLECULAR MEDIANTE rep-PCR DE AISLADOS NATIVOS DE Bacillus thuringiensis, OBTENIDOS DE MUESTRAS DE SUELO

    Directory of Open Access Journals (Sweden)

    Fabi\\u00E1n Galvis

    2014-01-01

    Full Text Available Bacillus thuringiensis es una bacteria Gram-positiva formadora de esporas, que produ - ce cristales parasporales de naturaleza proteica, tóxicos contra diferentes órdenes de insectos y biodegradables e inocuos para otras especies. Esta investigación empleó el modelo experimen - tal, que mediante técnicas de observación permi - tió, la identificación microbiológica y bioquímica de B. thuringiensis a partir de muestras de suelo de los municipios de Cúcuta, El Zulia, Los Patios, San Cayetano y Villa del Rosario, Norte de Santander, Colombia, y su posterior caracteri - zación con los marcadores moleculares Bc-Rep y MB1. Se identificaron microbiológica y bioquí - micamente 10 aislados como B. thuringiensis ; los resultados del análisis filogenético mostraron diferencias significativas en los agrupamientos obtenidos con los marcadores Bc-Rep y MB1. Con Bc-Rep se registró un índice de similaridad bajo (18%, mientras que con el marcador MB1 se obtuvo un índice mayor de similitud, 58%. En este trabajo se evidenció una gran variabilidad genética entre los aislados, que mostraron a los marcadores Bc-Rep y MB1 como altamente efectivos para diferenciar cepas estrechamente relacionadas, convirtiéndose en una herramienta genética de gran valor para estudios de identifi-cación y diversidad en B. thuringiensis.

  8. Operación Sonrisa República Dominicana: 8 años de una intensa y positiva experiencia

    Directory of Open Access Journals (Sweden)

    Fernando De La Cruz-Acosta

    2016-03-01

    Full Text Available El objetivo de este artículo es dar a conocer el trabajo que, desde hace 8 años, está realizando un grupo de cirujanos plásticos perteneciente a la Fundación Operación Sonrisa República Dominicana a favor de los pacientes con malformaciones congénitas faciales, especialmente labio leporino y fisura palatina.

  9. Operación Sonrisa República Dominicana: 8 años de una intensa y positiva experiencia

    OpenAIRE

    Fernando De La Cruz-Acosta

    2016-01-01

    El objetivo de este artículo es dar a conocer el trabajo que, desde hace 8 años, está realizando un grupo de cirujanos plásticos perteneciente a la Fundación Operación Sonrisa República Dominicana a favor de los pacientes con malformaciones congénitas faciales, especialmente labio leporino y fisura palatina.

  10. La Segunda República Española: Acción y omisión en materia electoral

    Directory of Open Access Journals (Sweden)

    Enrique Cebrián Zazurca

    2016-01-01

    Full Text Available : El artículo realiza un recorrido por la normativa electoral vigente en la Segunda República Española; presenta las características principales de las distintas normas, resaltando los elementos del régimen electoral republicano. Una vez conocido el régimen, se abordan las principales claves del sistema electoral de la República concebido globalmente. Finalmente, el artículo pone en relación el sistema electoral con el sistema de partidos, tratando de valorar si aquel influyó en la crisis de la República. El trabajo sostiene que en la Segunda República Española no se aprobó nunca una ley electoral que pudiera considerarse como verdaderamente republicana.

  11. Repetitive energy transfer from an inductive energy store

    Energy Technology Data Exchange (ETDEWEB)

    Honig, E.M.

    1984-01-01

    The theoretical and experimental results of a research program aimed at finding practical ways to transfer energy repetitively from an inductive energy store to various loads are discussed. The objectives were to investigate and develop the high power opening switches and transfer circuits needed to enable high-repetition-rate operation of such systems, including a feasibility demonstration at a current level near 10 kA and a pulse repetition rate of 1-10 kpps with a 1-ohm load. The requirements of nonlinear, time-varying loads, such as the railgun electromagnetic launcher, were also addressed. Energy storage capability is needed for proper power conditioning in systems where the duty factor of the output pulse train is low. Inductive energy storage is attractive because it has both a high energy storage density and a fast discharge capability. By producing a pulse train with a peak power of 75 MW at a pulse repetition rate of 5 kpps in a one-ohm load system, this research program was the first to demonstrate fully-controlled, high-power, high-repetition-rate operation of an inductive energy storage and transfer system with survivable switches. Success was made possible by using triggered vacuum gap switches as repetitive, current-zero opening switches and developing several new repetitive transfer circuits using the counterpulse technique.

  12. Skill learning in mirror reading: how repetition determines acquisition.

    Science.gov (United States)

    Ofen-Noy, N; Dudai, Y; Karni, A

    2003-07-01

    Practice makes perfect, but the role of repetitions in skill learning is not yet fully understood. For example, given a similar number of trials on a given task, it is debated whether repeating and non-repeating items are learned by the same neural process. When one is given training with both types of items--does one learn two separate skills, or only one? Here we show, using a mirror reading task, that practice trials with trial-unique words, and practice trials with repeated words, count towards learning to a different degree. There was no interaction between the time-course of learning repeated and unique words even within the same individuals given mixed training. While repeated words were learned faster than unique words, the repetitions-dependent gains diminished with training beyond a small number of repetitions. Moreover, the gains in performance could not be accounted for solely by the number of repetitions, as assumed by power-law models of learning; rather, the passage of time was a critical factor. Finally, our results suggest that although both repeated and new words were learned by both declarative and procedural memory mechanisms, even a single repetition of specific words could lead to the establishment of a selective differential representation in memory. The results are compatible with the notion of a repetition-sensitive process, triggered by specific repeating events. This 'repetition counter' may be a critical trigger for the effective formation of procedural as well as some type of declarative memory.

  13. El Ecoturismo en los Humedales: Análisis de las Potencialidades de República Dominicana

    Directory of Open Access Journals (Sweden)

    Francisco Orgaz Agüera

    2014-04-01

    Full Text Available El turismo se configura como uno de los principales motores económicos de República Dominicana. En este sentido, el turismo de sol y playa es la principal tipología turística en el país. Aunque, República Dominicana cuenta con numerosas potencialidades turísticas para poner en marchas nuevas formas de turismo: Turismo gastronómico, turismo ornitológico, turismo cultural, ecoturismo, etc. El principal objetivo de esta investigación es analizar las potencialidades existentes en República Dominicana para desarrollar el ecoturismo en las zonas de humedales. La metodología ha consistido en un trabajo de campo, a través de las entrevistas, los grupos de discusión y la observación participante. Los resultados muestran las grandes potencialidades que tiene el país para desarrollar el ecoturismo en los humedales, y contribuir así, al desarrollo socioeconómico de las comunidades locales. 

  14. Overview of the relativistic electron precipitations (REP) observed on LEO satellites and ISS by Bulgarian build instruments

    Science.gov (United States)

    Dachev, Tsvetan

    Relativistic electron precipitation (REP) are observed by the R3D B2/B3 and RD3-B3 instruments during the flights of the Foton M2/M3 and “BION-M” № 1 satellite in 2005, 2007 and 2013, and by the R3DE/R instruments at the EXPOSE-E facility of the European Columbus module and at the EXPOSE-R facility of the Russian Zvezda module of the International Space Station (ISS) in the period from February 2008 till August 2010. The obtained dose rates strongly depend by the external and internal shielding of the detectors in the instruments. The highest dose rate reaching more than 20 mGy h (-1) was observed outside the ISS Zvezda module during the REP in April 2010 being the second largest in GOES history with a >2 MeV electron fluence event. REP doses behind relatively thick shielding are too small but may play considerable role during extra vehicular activity (EVA) when the cosmonauts/astronauts body is shielded only by the space suit.

  15. Enhanced nicking activity of Rep in presence of pre-coat protein of Mungbean yellow mosaic India virus.

    Science.gov (United States)

    Rouhibakhsh, A; Choudhury, N R; Mukherjee, S K; Malathi, V G

    2012-04-01

    Yellow mosaic disease causes severe yield loss in grain legumes in Indian subcontinent and south east Asia. The disease is caused by two virus species, Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV). They have genome organization typical of Old World begomoviruses, the unique feature being the presence of an open reading frame (ORF) AV2 upstream of coat protein gene. In order to elucidate its function, ORF AV2 of blackgram isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Blackgram 3:1991] MYMIV-[IN:ND:Bg3:91] and cowpea isolate, Mungbean yellow mosaic India virus-[India:New Delhi:Cowpea7:1998] MYMIV-[IN:ND:Cp7:98], respectively, were over expressed in Escherichia coli in fusion with maltose binding protein (MBP). The recombinant protein did not show efficient binding to DNA. However, both MBP-BgAV2 and MBP-CpAV2 proteins modulated nicking and ATPase activity of replication initiation protein (Rep). Even low concentration, 20 ng of MBP-BgAV2 and MBP-CpAV2 could bring 20 folds increase in nicking activity of Rep. Similarly in the presence of AV2 protein, two to three fold increase in ATPase activity was observed. It is hypothesized that AV2 protein may play a role of accessory protein modulating Rep activities.

  16. Revolución versus reforma educativa en la segunda república española. Elementos de ruptura

    Directory of Open Access Journals (Sweden)

    Juan Manuel FERNÁNDEZ SORIA

    2010-03-01

    Full Text Available RESUMEN: Están fuera de toda duda las conexiones existentes entre la República de 1931-35 y la de 1936-39. En este sentido estamos de acuerdo con quienes afirman que el estallido de la guerra del 36 es el resultado de un proceso que arranca del mismo año en que se proclama la II República, iniciándose ya entonces —como sostiene Andrés M. Kramer— la «mecánica de guerra civil». La educación republicana en tiempo de guerra no puede quedar fuera de esta consideración, porque aquélla sumerge sus cimientos más profundos en la política educativa de 1931-35. Efectivamente, muchas de las acciones educativas y culturales llevadas a cabo durante la guerra no tendrían fácil explicación si no se examina previamente la tarea de la República del 31 en estos mismos aspectos.

  17. Directed PCR-free engineering of highly repetitive DNA sequences

    Directory of Open Access Journals (Sweden)

    Preissler Steffen

    2011-09-01

    Full Text Available Abstract Background Highly repetitive nucleotide sequences are commonly found in nature e.g. in telomeres, microsatellite DNA, polyadenine (poly(A tails of eukaryotic messenger RNA as well as in several inherited human disorders linked to trinucleotide repeat expansions in the genome. Therefore, studying repetitive sequences is of biological, biotechnological and medical relevance. However, cloning of such repetitive DNA sequences is challenging because specific PCR-based amplification is hampered by the lack of unique primer binding sites resulting in unspecific products. Results For the PCR-free generation of repetitive DNA sequences we used antiparallel oligonucleotides flanked by restriction sites of Type IIS endonucleases. The arrangement of recognition sites allowed for stepwise and seamless elongation of repetitive sequences. This facilitated the assembly of repetitive DNA segments and open reading frames encoding polypeptides with periodic amino acid sequences of any desired length. By this strategy we cloned a series of polyglutamine encoding sequences as well as highly repetitive polyadenine tracts. Such repetitive sequences can be used for diverse biotechnological applications. As an example, the polyglutamine sequences were expressed as His6-SUMO fusion proteins in Escherichia coli cells to study their aggregation behavior in vitro. The His6-SUMO moiety enabled affinity purification of the polyglutamine proteins, increased their solubility, and allowed controlled induction of the aggregation process. We successfully purified the fusions proteins and provide an example for their applicability in filter retardation assays. Conclusion Our seamless cloning strategy is PCR-free and allows the directed and efficient generation of highly repetitive DNA sequences of defined lengths by simple standard cloning procedures.

  18. State Assessment and Testing Programs: An Annotated ERIC Bibliography. Volume I: General References. Volume II: Individual State Programs.

    Science.gov (United States)

    Porter, Deborah Elena; Wildemuth, Barbara

    There is a growing body of literature in the ERIC data base pertaining to state educational assessment and testing programs. Volume I of this bibliography includes abstracts of 39 documents and journal articles describing the design and implementation of programs, as well as the technical and political issues which have been addressed by the…

  19. Super-resolved fluorescence microscopy: Nobel Prize in Chemistry 2014 for Eric Betzig, Stefan Hell, and William E. Moerner.

    Science.gov (United States)

    Möckl, Leonhard; Lamb, Don C; Bräuchle, Christoph

    2014-12-15

    A big honor for small objects: The Nobel Prize in Chemistry 2014 was jointly awarded to Eric Betzig, Stefan Hell, and William E. Moerner "for the development of super-resolved fluorescence microscopy". This Highlight describes how the field of super-resolution microscopy developed from the first detection of a single molecule in 1989 to the sophisticated techniques of today.

  20. 78 FR 39057 - In the Matter of the Designation of Eric Breininger as a Specially Designated Global Terrorist...

    Science.gov (United States)

    2013-06-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE In the Matter of the Designation of Eric Breininger as a Specially Designated Global Terrorist Pursuant To... aforementioned individual as a Specially Designated Global Terrorist pursuant to section 1(b) of the Order....

  1. Community Schools. ACSA School Management Digest, Series 1, Number 15. ERIC/CEM Research Analysis Series, Number 42.

    Science.gov (United States)

    Schofield, Dee

    Utilizing material in the ERIC network, this paper briefly investigates the theory, history, and current state of community schools. Planning guidelines for alternative uses of school facilities are discussed and some examples are given of effective shared facilities. Financial requirements for starting a community school and possible sources of…

  2. Adamson-Eric 100 : XX sajandi kunsti- ja kultuurilugu ühe inimsaatuse kaudu / Ülle Kruus

    Index Scriptorium Estoniae

    Kruus, Ülle

    2002-01-01

    Näitus "Adamson-Eric 100" Eesti Kunstimuuseumi Rüütelkonna hoones ja Adamson-Ericu muuseumis. Koostajad Ülle Kruus, Kersti Koll, Hanno Soans, kujundajad Leonhard Lapin (EKM), Jaak Soans (Adamson-Ericu muuseum). Adamson-Ericu teosed väljaspool Eestit ja Eestisse tagasi jõudnud tööd

  3. Repetition-based Interactive Facade Modeling

    KAUST Repository

    AlHalawani, Sawsan

    2012-07-01

    Modeling and reconstruction of urban environments has gained researchers attention throughout the past few years. It spreads in a variety of directions across multiple disciplines such as image processing, computer graphics and computer vision as well as in architecture, geoscience and remote sensing. Having a virtual world of our real cities is very attractive in various directions such as entertainment, engineering, governments among many others. In this thesis, we address the problem of processing a single fa cade image to acquire useful information that can be utilized to manipulate the fa cade and generate variations of fa cade images which can be later used for buildings\\' texturing. Typical fa cade structures exhibit a rectilinear distribution where in windows and other elements are organized in a grid of horizontal and vertical repetitions of similar patterns. In the firt part of this thesis, we propose an efficient algorithm that exploits information obtained from a single image to identify the distribution grid of the dominant elements i.e. windows. This detection method is initially assisted with the user marking the dominant window followed by an automatic process for identifying its repeated instances which are used to define the structure grid. Given the distribution grid, we allow the user to interactively manipulate the fa cade by adding, deleting, resizing or repositioning the windows in order to generate new fa cade structures. Having the utility for the interactive fa cade is very valuable to create fa cade variations and generate new textures for building models. Ultimately, there is a wide range of interesting possibilities of interactions to be explored.

  4. Understanding communicative actions: a repetitive TMS study.

    Science.gov (United States)

    Stolk, Arjen; Noordzij, Matthijs L; Volman, Inge; Verhagen, Lennart; Overeem, Sebastiaan; van Elswijk, Gijs; Bloem, Bas; Hagoort, Peter; Toni, Ivan

    2014-02-01

    Despite the ambiguity inherent in human communication, people are remarkably efficient in establishing mutual understanding. Studying how people communicate in novel settings provides a window into the mechanisms supporting the human competence to rapidly generate and understand novel shared symbols, a fundamental property of human communication. Previous work indicates that the right posterior superior temporal sulcus (pSTS) is involved when people understand the intended meaning of novel communicative actions. Here, we set out to test whether normal functioning of this cerebral structure is required for understanding novel communicative actions using inhibitory low-frequency repetitive transcranial magnetic stimulation (rTMS). A factorial experimental design contrasted two tightly matched stimulation sites (right pSTS vs left MT+, i.e., a contiguous homotopic task-relevant region) and tasks (a communicative task vs a visual tracking task that used the same sequences of stimuli). Overall task performance was not affected by rTMS, whereas changes in task performance over time were disrupted according to TMS site and task combinations. Namely, rTMS over pSTS led to a diminished ability to improve action understanding on the basis of recent communicative history, while rTMS over MT+ perturbed improvement in visual tracking over trials. These findings qualify the contributions of the right pSTS to human communicative abilities, showing that this region might be necessary for incorporating previous knowledge, accumulated during interactions with a communicative partner, to constrain the inferential process that leads to action understanding. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Epithelial topography for repetitive tooth formation

    Directory of Open Access Journals (Sweden)

    Marcia Gaete

    2015-12-01

    Full Text Available During the formation of repetitive ectodermally derived organs such as mammary glands, lateral line and teeth, the tissue primordium iteratively initiates new structures. In the case of successional molar development, new teeth appear sequentially in the posterior region of the jaw from Sox2+ cells in association with the posterior aspect of a pre-existing tooth. The sequence of molar development is well known, however, the epithelial topography involved in the formation of a new tooth is unclear. Here, we have examined the morphology of the molar dental epithelium and its development at different stages in the mouse in vivo and in molar explants. Using regional lineage tracing we show that within the posterior tail of the first molar the primordium for the second and third molar are organized in a row, with the tail remaining in connection with the surface, where a furrow is observed. The morphology and Sox2 expression of the tail retains characteristics reminiscent of the earlier stages of tooth development, such that position along the A-P axes of the tail correlates with different temporal stages. Sox9, a stem/progenitor cell marker in other organs, is expressed mainly in the suprabasal epithelium complementary with Sox2 expression. This Sox2 and Sox9 expressing molar tail contains actively proliferating cells with mitosis following an apico-basal direction. Snail2, a transcription factor implicated in cell migration, is expressed at high levels in the tip of the molar tail while E-cadherin and laminin are decreased. In conclusion, our studies propose a model in which the epithelium of the molar tail can grow by posterior movement of epithelial cells followed by infolding and stratification involving a population of Sox2+/Sox9+ cells.

  6. Repetitive transcranial magnetic stimulation in psychiatry

    Directory of Open Access Journals (Sweden)

    Biswa Ranjan Mishra

    2011-01-01

    Full Text Available Repetitive transcranial magnetic stimulation (rTMS is a non-invasive and relatively painless tool that has been used to study various cognitive functions as well as to understand the brain-behavior relationship in normal individuals as well as in those with various neuropsychiatric disorders. It has also been used as a therapeutic tool in various neuropsychiatric disorders because of its ability to specifically modulate distinct brain areas. Studies have shown that repeated stimulation at low frequency produces long-lasting inhibition, which is called as long-term depression, whereas repeated high-frequency stimulation can produce excitation through long-term potentiation. This paper reviews the current status of rTMS as an investigative and therapeutic modality in various neuropsychiatric disorders. It has been used to study the cortical and subcortical functions, neural plasticity and brain mapping in normal individuals and in various neuropsychiatric disorders. rTMS has been most promising in the treatment of depression, with an overall milder adverse effect profile compared with electroconvulsive therapy. In other neuropsychiatric disorders such as schizophrenia, mania, epilepsy and substance abuse, it has been found to be useful, although further studies are required to establish therapeutic efficacy. It appears to be ineffective in the treatment of obsessive compulsive disorder. There is a paucity of studies of efficacy and safety of rTMS in pediatric and geriatric population. Although it appears safe, further research is required to optimize its efficacy and reduce the side-effects. Magnetic seizure therapy, which involves producing seizures akin to electroconvulsive therapy, appears to be of comparable efficacy in the treatment of depression with less cognitive adverse effects.

  7. Clinical application of repetitive transcranial magnetic stimulation in stroke rehabilitation☆

    Science.gov (United States)

    Shin, Joonho; Yang, EunJoo; Cho, KyeHee; Barcenas, Carmelo L; Kim, Woo Jin; Min, Yusun; Paik, Nam-Jong

    2012-01-01

    Proper stimulation to affected cerebral hemisphere would promote the functional recovery of patients with stroke. Effects of repetitive transcranial magnetic stimulation on cortical excitability can be can be altered by the stimulation frequency, intensity and duration. There has been no consistent recognition regarding the best stimulation frequency and intensity. This study reviews the intervention effects of repetitive transcranial stimulation on motor impairment, dysphagia, visuospatial neglect and aphasia, and summarizes the stimulation frequency, intensity and area for repetitive transcranial magnetic stimulation to yield the best therapeutic effects. PMID:25745455

  8. Clinical application of repetitive transcranial magnetic stimulation in stroke rehabilitation

    Institute of Scientific and Technical Information of China (English)

    Joonho Shin; EunJoo Yang; KyeHee Cho; Carmelo L Barcenas; Woo Jin Kim; Yusun Min; Nam-Jong Paik

    2012-01-01

    Proper stimulation to affected cerebral hemisphere would promote the functional recovery of patients with stroke. Effects of repetitive transcranial magnetic stimulation on cortical excitability can be can be altered by the stimulation frequency, intensity and duration. There has been no consistent recognition regarding the best stimulation frequency and intensity. This study reviews the intervention effects of repetitive transcranial stimulation on motor impairment, dysphagia, visuospatial neglect and aphasia, and summarizes the stimulation frequency, intensity and area for repetitive transcranial magnetic stimulation to yield the best therapeutic effects.

  9. The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants

    Energy Technology Data Exchange (ETDEWEB)

    Hipp, Katharina; Rau, Peter; Schäfer, Benjamin [Institut für Biomaterialien und biomolekulare Systeme, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart (Germany); Gronenborn, Bruno [Institut des Sciences du Végétal, CNRS, 91198 Gif-sur-Yvette (France); Jeske, Holger, E-mail: holger.jeske@bio.uni-stuttgart.de [Institut für Biomaterialien und biomolekulare Systeme, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart (Germany)

    2014-08-15

    Geminiviruses, single-stranded DNA plant viruses, encode a replication-initiator protein (Rep) that is indispensable for virus replication. A potential cyclin interaction motif (RXL) in the sequence of African cassava mosaic virus Rep may be an alternative link to cell cycle controls to the known interaction with plant homologs of retinoblastoma protein (pRBR). Mutation of this motif abrogated rereplication in fission yeast induced by expression of wildtype Rep suggesting that Rep interacts via its RXL motif with one or several yeast proteins. The RXL motif is essential for viral infection of Nicotiana benthamiana plants, since mutation of this motif in infectious clones prevented any symptomatic infection. The cell-cycle link (Clink) protein of a nanovirus (faba bean necrotic yellows virus) was investigated that activates the cell cycle by binding via its LXCXE motif to pRBR. Expression of wildtype Clink and a Clink mutant deficient in pRBR-binding did not trigger rereplication in fission yeast. - Highlights: • A potential cyclin interaction motif is conserved in geminivirus Rep proteins. • In ACMV Rep, this motif (RXL) is essential for rereplication of fission yeast DNA. • Mutating RXL abrogated viral infection completely in Nicotiana benthamiana. • Expression of a nanovirus Clink protein in yeast did not induce rereplication. • Plant viruses may have evolved multiple routes to exploit host DNA synthesis.

  10. Shortening of subjective visual intervals followed by repetitive stimulation.

    Directory of Open Access Journals (Sweden)

    Fuminori Ono

    Full Text Available Our previous research demonstrated that repetitive tone stimulation shortened the perceived duration of the preceding auditory time interval. In this study, we examined whether repetitive visual stimulation influences the perception of preceding visual time intervals. Results showed that a time interval followed by a high-frequency visual flicker was perceived as shorter than that followed by a low-frequency visual flicker. The perceived duration decreased as the frequency of the visual flicker increased. The visual flicker presented in one hemifield shortened the apparent time interval in the other hemifield. A final experiment showed that repetitive tone stimulation also shortened the perceived duration of preceding visual time intervals. We concluded that visual flicker shortened the perceived duration of preceding visual time intervals in the same way as repetitive auditory stimulation shortened the subjective duration of preceding tones.

  11. The relationship between task repetition and language proficiency

    Directory of Open Access Journals (Sweden)

    Ahmad Mojavezi

    2014-01-01

    Full Text Available Task repetition is now considered as an important task-based implementation variable which can affect complexity, accuracy, and fluency of L2 speech. However, in order to move towards theorizing the role of task repetition in second language acquisition, it is necessary that individual variables be taken into account. The present study aimed to investigate the way task repetition correlates with language proficiency and the differential effects that task repetition might have on the complexity, accuracy, and fluency of L2 learners with different levels of proficiency. Fifty language learners of different levels of proficiency, selected from two different language centers, participated in this study. They were asked to perform an oral narrative task twice with a one-week interval. Results revealed that, compared to the participants with lower L2 proficiency, participants with higher levels of L2 proficiency produced more complex, accurate, and fluent speech on the second encounter with the same task.

  12. The repetition effect in building and construction works

    DEFF Research Database (Denmark)

    Gottlieb, Stefan Christoffer; Haugbølle, Kim

    are then applied on the Public Transport Authorities' main account structure of units and costs, and a method for assessing the possibilities of achieving effects of repetition for each account is described. Finally, the report summarises the core conditions necessary to take into consideration in relation......This report summarises the results from the work undertaken for the Public Transport Authority on the effect of learning and repetition in building and construction works. The results are applied by the Public Transport Authority in a new budgeting model, while the agency investigates...... the establishment of a new railway between Copenhagen and Ringsted. Drawing on an extensive literature review, the effect of repetition is determined to be in the range of 6-12 %. Further, the report identifies a series of factors affecting the possibilities of achieving effects of repetition. These factors...

  13. Neural dynamics during repetitive visual stimulation

    Science.gov (United States)

    Tsoneva, Tsvetomira; Garcia-Molina, Gary; Desain, Peter

    2015-12-01

    Objective. Steady-state visual evoked potentials (SSVEPs), the brain responses to repetitive visual stimulation (RVS), are widely utilized in neuroscience. Their high signal-to-noise ratio and ability to entrain oscillatory brain activity are beneficial for their applications in brain-computer interfaces, investigation of neural processes underlying brain rhythmic activity (steady-state topography) and probing the causal role of brain rhythms in cognition and emotion. This paper aims at analyzing the space and time EEG dynamics in response to RVS at the frequency of stimulation and ongoing rhythms in the delta, theta, alpha, beta, and gamma bands. Approach.We used electroencephalography (EEG) to study the oscillatory brain dynamics during RVS at 10 frequencies in the gamma band (40-60 Hz). We collected an extensive EEG data set from 32 participants and analyzed the RVS evoked and induced responses in the time-frequency domain. Main results. Stable SSVEP over parieto-occipital sites was observed at each of the fundamental frequencies and their harmonics and sub-harmonics. Both the strength and the spatial propagation of the SSVEP response seem sensitive to stimulus frequency. The SSVEP was more localized around the parieto-occipital sites for higher frequencies (>54 Hz) and spread to fronto-central locations for lower frequencies. We observed a strong negative correlation between stimulation frequency and relative power change at that frequency, the first harmonic and the sub-harmonic components over occipital sites. Interestingly, over parietal sites for sub-harmonics a positive correlation of relative power change and stimulation frequency was found. A number of distinct patterns in delta (1-4 Hz), theta (4-8 Hz), alpha (8-12 Hz) and beta (15-30 Hz) bands were also observed. The transient response, from 0 to about 300 ms after stimulation onset, was accompanied by increase in delta and theta power over fronto-central and occipital sites, which returned to baseline

  14. Platão e a poesia na República

    Directory of Open Access Journals (Sweden)

    Maria da Penha Villela-Petit

    2003-06-01

    Full Text Available Platão não é o iniciador, e sim o herdeiro da "velha divergência" entre filósofos e poetas. A motivação ético-teológica, que já animava Xenófanes,vai se reatualizar na República. Para poder apreciar o sentido dessa reatualização, importa que não se considere o Livro X isoladamente, mas se atente para os primeiros livros do diálogo. Neles, e com vistas a uma melhor determinação do justo -que é o que está em pauta-, Platão mostra a necessidade de se discutir as afirmações dos poetas. Trata-se assim de destituí-los da autoridade de que ainda gozam na educação e na opinião comum. Só graças à discussão filosófica e a uma educação por ela inspirada -o que pressupõe a produção ou a seleção de mitos- é que se pode esperar uma maior realização da justiça, tanto no plano do indivíduo (do governo de sua alma quanto no nível da cidade. A leitura aqui proposta das "razões" de Platão na República não impede que reconheçamos a importância da tragédia para a compreensão da existência humana, inclusive no que toca à idéia do divino.Plato is not the initiator, but rather the heir of the "old quarrel", between philosophers and poets. The ethical and theological motivation that drove Xenophanes is brought to date in the Republic. In order to realize that, one must take into account the whole of the dialogue - where the definition of justice is at stake - and, more precisely, its first books. There, Plato shows how important it is to put the poets' claims to question. The point is, after all, to deprive them of the authority they enjoy in what concerns educational matters as well as with public opinion. It is only through an education which is inspired by philosophical questioning - which also implies the production and selection of myths - that one can expect more justice both on the individual (the gouvernment of his soul and on the city levels. The reading we propose of Plato's "reasons" in the Republic does

  15. Autism and exergaming: effects on repetitive behaviors and cognition

    OpenAIRE

    Anderson-Hanley C; Tureck K; Schneiderman RL

    2011-01-01

    Cay Anderson-Hanley, Kimberly Tureck, Robyn L Schneiderman Department of Psychology, Union College, Schenectady, NY, USA Abstract: Autism is a neurodevelopmental disorder that leads to impairment in social skills and delay in language development, and results in repetitive behaviors and restricted interests that impede academic and social involvement. Physical exercise has been shown to decrease repetitive behaviors in autistic children and improve cognitive function across the life-span. Ex...

  16. Breakdown behavior of electronics at variable pulse repetition rates

    OpenAIRE

    Korte, S.; H. Garbe

    2006-01-01

    The breakdown behavior of electronics exposed to single transient electromagnetic pulses is subject of investigations for several years. State-of-the-art pulse generators additionally provide the possibility to generate pulse sequences with variable pulse repetition rate. In this article the influence of this repetition rate variation on the breakdown behavior of electronic systems is described. For this purpose microcontroller systems are examined during line-led exposure to pulses with repe...

  17. Analysis of repetitive DNA in chromosomes by flow cytometry.

    Science.gov (United States)

    Brind'Amour, Julie; Lansdorp, Peter M

    2011-06-01

    We developed a flow cytometry method, chromosome flow fluorescence in situ hybridization (FISH), called CFF, to analyze repetitive DNA in chromosomes using FISH with directly labeled peptide nucleic acid (PNA) probes. We used CFF to measure the abundance of interstitial telomeric sequences in Chinese hamster chromosomes and major satellite sequences in mouse chromosomes. Using CFF we also identified parental homologs of human chromosome 18 with different amounts of repetitive DNA.

  18. Linear- and Repetitive-Feature Detection Within Remotely Sensed Imagery

    Science.gov (United States)

    2017-04-01

    1.1 Background The Army desires the ability to deliver cargo, equipment, and personnel to harsh locations almost anywhere on the planet . This...because the Hough transform is designed to look for straight linear features, which most real- life fea- tures are not. As mention previously, it is...repetitive features are differentiated based on their appearance in the images of interest; however, real- life repetitive features often corre- spond to

  19. Linear- and Repetitive Feature Detection Within Remotely Sensed Imagery

    Science.gov (United States)

    2017-04-01

    1.1 Background The Army desires the ability to deliver cargo, equipment, and personnel to harsh locations almost anywhere on the planet . This...because the Hough transform is designed to look for straight linear features, which most real- life fea- tures are not. As mention previously, it is...repetitive features are differentiated based on their appearance in the images of interest; however, real- life repetitive features often corre- spond to

  20. Brain Injury Following Repetitive Apnea in Newborn Piglets

    Science.gov (United States)

    Schears, Gregory; Creed, Jennifer; Antoni, Diego; Zaitseva, Tatiana; Greeley, William; Wilson, David F.; Pastuszko, Anna

    Repetitive apnea is associated with a significant increase in extracellular dopamine, generation of free radicals as determined by o-tyrosine formation and increase in Fluoro-Jade staining of degenerating neurons. This increase in extracellular dopamine and of hydroxyl radicals in striatum of newborn brain is likely to be at least partly responsible for the neuronal injury and neurological side effects of repetitive apnea.

  1. Illusory sensation of movement induced by repetitive transcranial magnetic stimulation

    DEFF Research Database (Denmark)

    Christensen, Mark Schram; Lundbye-Jensen, J.; Grey, M.J.;

    2010-01-01

    Human movement sense relies on both somatosensory feedback and on knowledge of the motor commands used to produce the movement. We have induced a movement illusion using repetitive transcranial magnetic stimulation over primary motor cortex and dorsal premotor cortex in the absence of limb moveme...... premotor cortex stimulation was less affected by sensory and motor deprivation than was primary motor cortex stimulation. We propose that repetitive transcranial magnetic stimulation over dorsal premotor cortex produces a corollary discharge that is perceived as movement....

  2. Discurso de orden.Tribunal supremo de justicia de la república Bolivariana de Venezuela

    Directory of Open Access Journals (Sweden)

    José Manuel Delgado Ocando

    2001-01-01

    Full Text Available José Manuel Delgado Ocando, nos presenta un original y brillante análisis de la realidad política venezolana. Bien es sabido que a raíz de la elección presidencial de Hugo Chávez Frías, éste convoca a un referéndum popular con la expresa intención de constituir un nuevo orden constitucional. Tal inusual experiencia de la ciudadanía en la participación de los “asuntos de Estado”, generó no pocas controversias sobre el conflicto de competencias entre la Asamblea Nacional Constituyente (poder constituyente y el Congreso de la República (poder constituido. No previsto en la Constitución de 1961 este inédito protagonismo popular y la coexistencia de dos regímenes constitucionales distintos, le toca a la Corte Suprema de Justicia legislar sobre este caso particular teniendo que apelar a la doctrina de la supraconstitucionalidad a fin de dirimir el conflicto normativo y ratificar la legalidad de la consulta referendaria que da origen a la Asamblea Nacional Constituyente. El autor se vale de una compleja hermenéutica jurídico-política para interpretar este inédito fenómeno de la filosofía política moderna que se ha vivido en Venezuela, así como para confirmar su confianza en la producción originaria del derecho cuando ésta es reclamada por la soberanía popular.

  3. "Sobre la justicia en el libro "La República" de Platón"

    Directory of Open Access Journals (Sweden)

    Walter Olivari

    2008-01-01

    Full Text Available La justicia, ha sido siempre un tema presente en la filosofía y con más notoriedad en la filosofía clásica, por ello resulta saludable, atractivo y de valor el análisis de los textos griegos al respecto. En este punto, Platón es quizás el más estudiado, siempre sus diálogos resultan útiles para quien recorre sus páginas, ya convertidas en verdaderos monumentos de la filosofía, con pasajes y afirmaciones hasta hoy irrefutables, además haciendo de su maestro Sócrates un personaje invalorable para la sabiduría universal. La innata ansia por una vida mejor, empuja al hombre casi siempre a la búsqueda de un mundo sin defectos. Esta es la razón de sus ilusiones utópicas de alcanzar justica, entendida ella como la perfecta armonía en la convivencia social. De la producción platónica, extensa, variada y valiosa, por todo lo que trata y por su sobrevivencia al paso de los años, la obra La República ocupa un lugar de preferencia, por eso estudiar sus paginas y repasar, tanto como re-pensar sus conceptos, es buen estimulo para quienes desean conocer temas de trascendencia en Filosofía, Derecho o afines. Volver a los clásicos es siempre un deber, ellos siempre son fascinantes y sus enseñanzas perduran con gran lozanía, a prueba de todo elemento corrosivo, por tanto no hay pretexto que valga, como para no estudiar, lo que sobre la justicia con genialidad nos comentaron hace ya tantos años.

  4. High burnup effects on fuel behaviour under accident conditions: the tests CABRI REP-Na

    Science.gov (United States)

    Schmitz, Franz; Papin, Joelle

    A large, performance based, knowledge and experience in the field of nuclear fuel behaviour is available for nominal operation conditions. The database is continuously completed and precursor assembly irradiations are performed for testing of new materials and innovative designs. This procedure produces data and arguments to extend licencing limits in the permanent research for economic competitiveness. A similar effort must be devoted to the establishment of a database for fuel behaviour under off-normal and accident conditions. In particular, special attention must be given to the so-called design-basis-accident (DBA) conditions. Safety criteria are formulated for these situations and must be respected without consideration of the occurrence probability and the risk associated to the accident situation. The introduction of MOX fuel into the cores of light water reactors and the steadily increasing goal burnup of the fuel call for research work, both experimental and analytical, in the field of fuel response to DBA conditions. In 1992, a significant programme step, CABRI REP-Na, has been launched by the French Nuclear Safety and Protection Institute (IPSN) in the field of the reactivity initiated accident (RIA). After performing the nine experiments of the initial test matrix it can be concluded that important new findings have been evidenced. High burnup clad corrosion and the associated degradation of the mechanical properties of the ZIRCALOY4 clad is one of the key phenomena of the fuel behaviour under accident conditions. Equally important is the evidence that transient, dynamic fission gas effects resulting from the close to adiabatic heating introduces a new explosive loading mechanism which may lead to clad rupture under RIA conditions, especially in the case of heterogeneous MOX fuel.

  5. Grade repetition in primary school from teachers’ perspective

    Directory of Open Access Journals (Sweden)

    Malinić Dušica

    2011-01-01

    Full Text Available School underachievement is exhibited gradually, in different forms, while grade repetition figures as one of the most prominent forms of underachievement. In order to observe this phenomenon from different perspectives, we conducted a research aimed at identifying teacher attitudes towards grade repetition and grade repeaters in primary school, based on their perceptions of: (a the cause of grade repetition; (b the responsibility for grade repetition and (c grade repetition as an educational measure. The administered questionnaire was constructed for the purposes of the research, descriptive statistics was used, and data were obtained on the sample of 136 teachers from 31 primary schools from the territory of the City of Belgrade. The results point out to the conclusion that teachers perceive grade repetition as, first and foremost, the consequence of students’ lack of interest in school and learning and undisciplined behavior in class. By treating student underachievement mainly as a consequence of laziness, lack of motivation and insufficient effort, teachers transfer responsibility to others, assessing that the personal degree of responsibility for the underachievement of their students is very low. The responsibility for underachievement is perceived more as a problem of the student, his/her family, peer group, than as the problem of teachers themselves. The concluding part points out to certain teaching procedures and methods that have proved to be useful in the prevention of student underachievement.

  6. Properties of water surface discharge at different pulse repetition rates

    Energy Technology Data Exchange (ETDEWEB)

    Ruma,; Yoshihara, K. [Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555 (Japan); Hosseini, S. H. R., E-mail: hosseini@kumamoto-u.ac.jp; Sakugawa, T.; Akiyama, H. [Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555 (Japan); Institute of Pulsed Power Science, Kumamoto University, Kumamoto 860-8555 (Japan); Akiyama, M. [Department of Electrical and Electronic Engineering, Kagoshima University, Kagoshima 890-0065 (Japan); Lukeš, P. [Institute of Plasma Physics, AS CR, Prague, Prague 18200 (Czech Republic)

    2014-09-28

    The properties of water surface discharge plasma for variety of pulse repetition rates are investigated. A magnetic pulse compression (MPC) pulsed power modulator able to deliver pulse repetition rates up to 1000 Hz, with 0.5 J per pulse energy output at 25 kV, was used as the pulsed power source. Positive pulse with a point-to-plane electrode configuration was used for the experiments. The concentration and production yield of hydrogen peroxide (H₂O₂) were quantitatively measured and orange II organic dye was treated, to evaluate the chemical properties of the discharge reactor. Experimental results show that the physical and chemical properties of water surface discharge are not influenced by pulse repetition rate, very different from those observed for under water discharge. The production yield of H₂O₂ and degradation rate per pulse of the dye did not significantly vary at different pulse repetition rates under a constant discharge mode on water surface. In addition, the solution temperature, pH, and conductivity for both water surface and underwater discharge reactors were measured to compare their plasma properties for different pulse repetition rates. The results confirm that surface discharge can be employed at high pulse repetition rates as a reliable and advantageous method for industrial and environmental decontamination applications.

  7. The future of immunization policies in Italy and in the European Union: The Declaration of Erice.

    Science.gov (United States)

    Odone, Anna; Fara, Gaetano M; Giammaco, Giuseppe; Blangiardi, Francesco; Signorelli, Carlo

    2015-01-01

    On December 2014 the Employment, Social Policy, Health and Consumer Affairs Council (EPSCO) of the European Union adopted the Council Conclusions on "Vaccinations as an effective tool in public health," a crucial step to strengthen EU action supporting Member States (MS) to implement effective immunization policies and programs. As a contribution to the ongoing pan-European discussion and to the Italian commitment to stay at the forefront of promoting vaccination policies, the Erice Declaration was drafted by Italy's best experts in the field of immunization to transpose to the national level the goals set by the EPSCO Conclusions. The aim of the current letter is to present to the broader international audience the Italian perspective as a case study to assess different immunization policy models, challenges and priorities.

  8. Nobel Prize Recipient Eric Betzig Presents Lecture on Efforts to Improve High-Resolution Microscopy | Poster

    Science.gov (United States)

    Eric Betzig, Ph.D., a 2014 recipient of the Nobel Prize in Chemistry and a scientist at Janelia Research Campus (JRC), Howard Hughes Medical Institute, in Ashburn, Va., visited NCI at Frederick on Sept. 10 to present a Distinguished Scientist lecture and discuss the latest high-resolution microscopy techniques. Betzig co-invented photoactivation localization microscopy (PALM) in collaboration with scientists at NIH. PALM achieves 10-fold improvement in spatial resolution of cells, going from the resolution limit of approximately 250 nm in standard optical microscopy down to approximately 20 nm, thus producing a so-called “super-resolution” image. Spatial resolution refers to the clarity of an image or, in other words, the smallest details that can be observed from an image.

  9. Nobel Prize Recipient Eric Betzig Presents Lecture on Efforts to Improve High-Resolution Microscopy | Poster

    Science.gov (United States)

    Eric Betzig, Ph.D., a 2014 recipient of the Nobel Prize in Chemistry and a scientist at Janelia Research Campus (JRC), Howard Hughes Medical Institute, in Ashburn, Va., visited NCI at Frederick on Sept. 10 to present a Distinguished Scientist lecture and discuss the latest high-resolution microscopy techniques. Betzig co-invented photoactivation localization microscopy (PALM) in collaboration with scientists at NIH. PALM achieves 10-fold improvement in spatial resolution of cells, going from the resolution limit of approximately 250 nm in standard optical microscopy down to approximately 20 nm, thus producing a so-called “super-resolution” image. Spatial resolution refers to the clarity of an image or, in other words, the smallest details that can be observed from an image.

  10. Debating Modernity as Secular Religion: Hans Kelsen’s futile exchange with Eric Voegelin

    DEFF Research Database (Denmark)

    Thomassen, Bjørn

    2014-01-01

    This article reviews the mysterious and recently published last book by Hans Kelsen, “Secular Religion. A Polemic Against the Misinterpretation of Modern Social Philosophy, Science and Politics as ‘New Religions’”, contextualizing it with reference to the little known dialogue between Hans Kelsen...... and Eric Voegelin. The confrontation between Kelsen and Voegelin, two of the most illustrious émigré scholars who found in America their new home, is important to revisit because it touches upon several axes of debate of crucial importance to postwar intellectual history: the religion/secularity debate......, the positivist/anti-positivist debates, and the controversy that also led to the famous Voegelin/Arendt debate: how to read the horrors of totalitarianism into a historical trajectory of modernity. Although the Kelsen/Voegelin exchange ended in failure and bitterness its substance matter goes to the heart...

  11. Eric Wen

    Institute of Scientific and Technical Information of China (English)

    CLAIRE LI

    2008-01-01

    @@ How did you come to open a snowboard shop? I attended Shenyang Sports University,and was very interested in sports photography.During my second year of university,I worked part-time for a snowboard company and started getting to know about snowboarding and skiing.I gradually found snowboarding as intriguing as photography and it became one of my main hobbies.After working for the company part-time for three years,I graduated and came to Shanghai.

  12. Place field repetition and spatial learning in a multicompartment environment.

    Science.gov (United States)

    Grieves, Roddy M; Jenkins, Bryan W; Harland, Bruce C; Wood, Emma R; Dudchenko, Paul A

    2016-01-01

    Recent studies have shown that place cells in the hippocampus possess firing fields that repeat in physically similar, parallel environments. These results imply that it should be difficult for animals to distinguish parallel environments at a behavioral level. To test this, we trained rats on a novel odor-location task in an environment with four parallel compartments which had previously been shown to yield place field repetition. A second group of animals was trained on the same task, but with the compartments arranged in different directions, an arrangement we hypothesised would yield less place field repetition. Learning of the odor-location task in the parallel compartments was significantly impaired relative to learning in the radially arranged compartments. Fewer animals acquired the full discrimination in the parallel compartments compared to those trained in the radial compartments, and the former also required many more sessions to reach criterion compared to the latter. To confirm that the arrangement of compartments yielded differences in place cell repetition, in a separate group of animals we recorded from CA1 place cells in both environments. We found that CA1 place cells exhibited repeated fields across four parallel local compartments, but did not do so when the same compartments were arranged radially. To confirm that the differences in place field repetition across the parallel and radial compartments depended on their angular arrangement, and not incidental differences in access to an extra-maze visual landmark, we repeated the recordings in a second set of rats in the absence of the orientation landmark. We found, once again, that place fields showed repetition in parallel compartments, and did not do so in radially arranged compartments. Thus place field repetition, or lack thereof, in these compartments was not dependent on extra-maze cues. Together, these results imply that place field repetition constrains spatial learning.

  13. 白灵菇和杏鲍菇亲缘关系的ERIC-PCR技术分析%Analysis of the Phylogenetic Relationship Between Pleurotus nebrodensis and Pleurotus eryngi Strains by ERIC-PCR Technique

    Institute of Scientific and Technical Information of China (English)

    周金凤; 姚强; 张翠霞; 刘林德; 高兴喜

    2010-01-01

    采用ERIC-PCR技术对白灵菇(Pleurotus nebrodensis)和杏鲍菇(Pleurotus eryngi)两菌种19个栽培菌株进行了亲缘关系分析.结果表明,ERIC-PCR技术能够有效地区分这两个菌种:在76%的相似水平上,白灵菇与杏鲍菇各不同菌株分别聚为一组,其中杏鲍菇各菌株间出现较为丰富的遗传多样性;白灵菇各菌株间亲缘关系较近,遗传多样性较低,许多菌株可能是同株异名菌株.

  14. Hacia una república conservadora : el programa político del Partido Agrario Español

    Directory of Open Access Journals (Sweden)

    Luis Teófilo Gil Cuadrado

    2005-01-01

    Full Text Available El Partido Agrario Español (PAE, fundado en enero de 1934 y presidido por José Martínez de Velasco, representó, dentro de la II República española, una alternativa conservadora y, al tiempo, leal al régimen. El programa político del PAE aceptaba la República —a diferencia de otros partidos derechistas del momento, como la CEDA y los monárquicos— y defendía la unidad nacional, el establecimiento de una segunda Cámara legislativa, la protección de la agricultura y la industria mediante aranceles, el libre comercio dentro de las fronteras nacionales y la revisión de algunas leyes del primer bienio como la Reforma Agraria de 1932. El objetivo era la configuración de una República conservadora distinta, en aspectos fundamentales, a la diseñada por la Constitución de 1931 y la legislación del bienio social-republicano.The Spanish Agrarian Party (Partido Agrario Español, PAE, founded in january of 1934 and presided by José Martínez de Velasco, was, in the political life of the Spanish Second Republic, a conservative and republican option. The agrarian program accepted the republican regime —in opposition to other conservative parties, like the CEDA or the monarchists— and defended the national union, the establishment of a Senate, the protection of the national agriculture and industry trough tariffs, the free trade in the Spanish market and the revision of some laws voted in the first biennium like the Agrarian Reform, promulgated in 1932. The idea was to establish a conservative Republic, different, in some fundamental aspects, of the regime designed by the Constitution of 1931 and the legislation approved during the socialist-republican biennium.

  15. Proyectos de parcelas con servicios desarrollados por el INVI en la República Dominicana

    Directory of Open Access Journals (Sweden)

    Pou Howley, Carlos

    1984-07-01

    Full Text Available Santo Domingo, the capital of the Dominican Republic, has not escaped the phenomenon of urban growth and the concentration of large slum areas or hovels, mainly due to the migratory movements of the peasants who have been driven from the country. As a viable solution to the enormous problem of housing in this city, INVI, with the participation of the World Bank, is putting forward projects for lots supplied with services in which infrastructure services are installed on urban land and plots or parcels of land are given to families so that they can build or complete their houses. These projects are located in places registering a greater tendency towards development. The urban plan formulated in both projects is of an eminently pedestrian nature with a reduced thoroughfare for vehicles. In the pedestrian precinct, it was possible to lay down what have been called mini-parks, which involve widening the pedestrian thoroughfare and, in addition to constituting green areas distributed throughout the projects, they also reinforce the idea of a neighbourhood. Because of the scope of the projects, a series of installations have been provided to maintain their own community services, as well as commercial and institutional ones, which are distributed strategically.Santo Domingo, capital de la República Dominicana, no ha escapado al fenómeno del crecimiento urbano y a la concentración de grandes tugurios o arrabales, debido principalmente a los movimientos migratorios de los campesinos expulsados del campo. Como una solución viable al gran problema de la vivienda de esta ciudad, el INVI, con la participación del Banco Mundial, plantea proyectos de parcelas con Servicios en los cuales se instalan servicios de infraestructura en tierras urbanas y se proporcionan parcelas o solares a familias para que éstas construyan o completen sus viviendas. Se ubican estos proyectos en sitios que acusan una mayor tendencia de desarrollo. El esquema urbano

  16. A natureza dos números na República de Platão

    Directory of Open Access Journals (Sweden)

    Anastácio Borges de Araújo Jr.

    2010-12-01

    Full Text Available É incontestável a presença das matemáticas nos diálogos de Platão, todavia o alcance e o sentido desta presença é matéria de controvérsia entre os especialistas. Alguns acreditam que os temas matemáticos empregados nos diálogos são fantasias matemáticas, outros defendem baseados, sobretudo no testemunho de Aristóteles, que Platão teria substituído na sua hipótese as formas inteligíveis pelos números ideais. Sem tomar partido de antemão nestas querelas, o que propomos aqui é uma investigação acerca da natureza dos números fundamentada exclusivamente nos textos de Platão, particularmente orientada por uma passagem do livro VII do diálogo República(525 b11- c3, em que Sócrates diz o seguinte: "Seria, portanto, conveniente colocar este estudo, ò Glaucon, dentro de uma legislação e persuadir aqueles que vão participar das grandes coisas na cidade a irem ao cálculo e a aplicarem-se a ele, não nos seus afazeres privados, mas até chegarem à contemplação da natureza dos números através da própria intelecção (...".The presence of the mathematics in Plato's dialogues is unquestionable. However, its reach and sense is controversial for some specialists. Some believe that the mathematical themes employed in the dialogues are mathematical fantasies; on the other hand, others defend, based on the Aristotle's testimonies, that Plato would have substituted the intelligible forms by the ideal numbers in his hypothesis. Having not taken part in the quarrel, we propose an investigation on the nature of the numbers based exclusively in Plato's texts, particularly oriented by the a passage in book VII of Republic (525 b11 - c3, in which Socrates says: "Then it would be fitting, Glaucon, to set this study down in law and persuade those who are going to participate in the greatest things in the city to go to calculation and to take it up, not after the fashion of private men, but stay with it until they come to the

  17. Galeguismo, agro e agrarismo na Galicia da II República

    Directory of Open Access Journals (Sweden)

    Cabo Villaverde, Miguel

    2009-12-01

    Full Text Available The Partido Galeguista (PG was created in December 1931 as the expression of Galician nationalism during the Second Republic (1931-1936. Prior to that, the Galician nationalists had been very influential in the cultural sphere but rather powerless from a political point of view. The PG regarded agriculture and peasantry as a priority, not only because of their objective importance in the Galician economy and society in the 1930s but also because they were seen as the bulwarks of the national identity. In fact, the characteristics, needs and paths of development of the Galician agrarian sector were usually defined as opposed to those of the rest of Spain. It is then understable that the PG tried to win the agrarian unions over, and that it is arguable that it had plenty of common features with the so-called Agrarian Parties in other European regions.

    O Partido Galeguista (PG foi creado en decembro de 1931 como expresión do nacionalismo galego durante a Segunda República (1931-1936. Con anterioridade, os nacionalistas galegos foron moi influíntes no eido cultural pero maiormente inoperantes dende o punto de vista político. O PG consideraba a agricultura e o campesiñado como unha prioridade, non só pola súa importancia obxectiva na economía e sociedade galegas dos anos trinta senón tamén porque eran vistos como os baluartes da identidade nacional. De feito, as características, necesidades e vías de desenvolvemento do sector agrario galego eran normalmente definidas en oposición ás do resto de España. É daquela comprensible que o PG tentase gañarse ás sociedades agrarias, e mesmo se pode afirmar que tiña moitos puntos en común cos chamados Partidos Agrarios doutras rexións europeas.

  18. Virtud en La República de Platón y El Príncipe de Maquiavelo

    OpenAIRE

    2014-01-01

    Desde el punto de vista de la ética de virtudes, el presente ensayo compara los filósofos-reyes de Platón con el príncipe de Maquiavelo. Primeramente, se define la ética de virtudes; seguidamente, se explica la manera en que se manifiesta la virtud en La República de Platón y El Príncipe de Maquiavelo. Finalmente, se valoran las semejanzas y diferencias entre ambos autores con respecto al concepto de virtud.

  19. Proteinopatías amiloides: desarrollo de un nuevo sistema modelo en microorganismos basado en RepA-WH1

    OpenAIRE

    Gasset Rosa, Fátima

    2012-01-01

    Las bases moleculares de la agregación de las proteínas amiloides son similares en todos los organismos, sean provenientes de microorganismos procariotas, hongos o metazoos. Muchas de estas proteínas poseen naturaleza priónica y son agentes causantes de enfermedades fatales (neurodegenerativas o sistémicas), cuyos mecanismos quedan todavía por esclarecer como previo paso hacia posibles terapias. Considerando estos presupuestos, el estudio de la amiloidosis de RepA-WH1, una proteína iniciadora...

  20. O surgimento da escola médica paulista no contexto brasileiro da Primeira República (1891 a 1930

    Directory of Open Access Journals (Sweden)

    Márcia Regina Silva Barros da

    2004-01-01

    Full Text Available Este trabajo intenta compreender el proceso de instauración de la enseñanza médica en São Paulo, Brasil, entre finales del siglo XIX y principios del siglo XX. Para tanto se discute los cambios que ocurrieron en el Estado de São Paulo en los comienzos del periodo de la República, en el contexto de la creación de nuevas instituciones médicas, tales como la Sociedade de Medicina e Cirurgia y el Serviço Sanitário, asi como surgimiento de varias revistas de la area.