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  1. Evaluation of morphological changes of the skin after radiation-induced injury in Wistar rats;Avaliacao de alteracoes morfologicas da pele apos lesao radioinduzida em ratos Wistar

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    Andrade, Cherley Borba Vieira de

    2010-07-01

    The cancer covers a heterogeneous group of more than 100 diseases with different etiology and prognosis. Radiotherapy is one of the most commonly used treatment modalities, aiming at the destruction of cancer cells, using ionizing radiation. One of the limiting factors of radiotherapy is that radiation promotes the death of tumor cells in addition to injure healthy tissue neighboring the tumor, and may cause their death. Irradiation of the skin, accidental or for therapeutic purposes can trigger many injuries culminating in fibrosis, which implies functional alteration of the body. The evaluation of morphological effects associated with skin irradiation becomes essential to develop more effective radiation strategies and decreased morbidity; and in case of accidents, proper handling of the victim.Evaluate radio-induced dermal changes using a Wistar rats model irradiated with 10, 40 and 60Gy. Male Wistar rats, aged approximately three months, were pre-anesthetized with midazolam and xylazine and anesthetized with sodium pentobarbital, shaved in the back, immobilized on polystyrene support in the prone position and irradiated with doses of 10, 40 and 60 Gy, with 4MeV nominal energy electron beams. The skin was irradiated in a 3cm{sup 2} field, and used 0.5cm of tissue equivalent material, to obtain a homogeneous dose distribution. After irradiation, the animals remained on constant evaluation, and the lesions were recorded photographically. The animals were divided into groups and were killed on the irradiation day, 5, 10, 15, 25 and 100 days after irradiation. The skin was fixed in 10% formaldehyde; the samples were embedded in paraffin and cut. The sections were stained with hematoxylin-eosin, picrosirius red and immuno stained with antibody anti-TGF beta1. Another part of the tissue was fixed in 2.5% glutaraldehyde and processed for scanning electron microscopy. It was observed macroscopically the appearance of skin lesions similar to burns on the entire

  2. Avaliacao de projeto de promocao da saude para adolescentes

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    Jovino Oliveira Ferreira

    2013-06-01

    Full Text Available OBJETIVO: Avaliar programa de promoção de atividade física e de escolhas alimentares entre adolescentes. MÉTODOS: Estudo transversal realizado com 911 adolescentes de 13 a 18 anos de idade, de escolas públicas de Goiânia, GO, em 2010. Os participantes foram divididos em dois grupos: intervenção (escolas participantes do Projeto Viver Saudável e controle. Foram considerados como atividade física: deslocamento, aulas de educação física na escola, atividade física fora da escola e no lazer. Foram definidos como ativos aqueles que acumularam 300 ou mais minutos por semana. Quanto às escolhas alimentares, foi classificado como adequado o consumo de alimentos protetores em cinco ou mais dias da semana. Foram realizadas análises estatísticas com teste de Qui-quadrado de Pearson. RESULTADOS: A maioria dos adolescentes foi identificada como inativos ou insuficientemente ativos, com 65,7% no grupo intervenção e 65,2% no grupo controle, sem diferenças significativas entre os grupos e com prevalência maior entre o sexo feminino. Pouco mais da metade dos adolescentes consumiam alimentos protetores da saúde em cinco ou mais dias da semana, sendo 56,6% e 50,4%, respectivamente, nos grupos intervenção e controle (p = 0,373. CONCLUSÕES: A ausência de diferenças quanto a escolhas alimentares e prática de atividade física entre os grupos intervenção e controle indica que o projeto Viver Saudável precisa ser reavaliado visando melhorar a efetividade no cumprimento dos seus objetivos.

  3. Avaliacao de interacoes medicamentosas potenciais em prescricoes da atencao primaria de Vitoria da Conquista (BA), Brasil

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    Danyllo Fabio Lessa Leao; Cristiano Soares de Moura; Danielle Souto de Medeiros

    2014-01-01

    As interações medicamentosas são fatores de risco para a ocorrência de reações adversas a medicamentos. Este estudo teve o objetivo de avaliar as interações medicamentosas potenciais em prescrições da atenção primária de Vitória da Conquista (BA), visando preencher a lacuna de conhecimento sobre essa temática no Brasil. Foram coletadas informações sobre diversas variáveis de prescrições oriundas da atenção primária e as interações medicamentosas avaliadas a partir dos bancos de dados do Medsc...

  4. Instrumentos em Lingua Brasileira de Sinais para avaliacao da qualidade de vida da populacao surda

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    Neuma Chaveiro

    2013-06-01

    Full Text Available OBJETIVO: Construir a versão em Língua Brasileira de Sinais dos instrumentos WHOQOL-BREF e WHOQOL-DIS para avaliar a qualidade de vida da população surda brasileira. MÉTODOS: Utilizou-se metodologia proposta pela Organização Mundial da Saúde (WHOQOL-BREF e WHOQOL-DIS para a construção dos instrumentos adaptados para população surda em Língua Brasileira de Sinais (Libras. A pesquisa para execução do instrumento consistiu de 13 etapas: 1 criação do sinal qualidade de vida; 2 desenvolvimento das escalas de respostas em Libras; 3 tradução por um grupo bilíngue; 4 versão reconciliadora; 5 primeira retrotradução; 6 produção da versão em Libras a ser disponibilizada aos grupos focais; 7 realização dos grupos focais; 8 revisão por um grupo monolíngue; 9 revisão pelo grupo bilíngue; 10 análise sintática/semântica e segunda retrotradução; 11 reavaliação da retrotradução pelo grupo bilíngue; 12 filmagem da versão para o software; 13 desenvolvimento do software WHOQOL-BREF e WHOQOL-DIS em Libras. RESULTADOS: Características peculiares da cultura da população surda apontaram a necessidade de adaptações na metodologia de aplicação de grupos focais quando compostos por pessoas surdas. As convenções ortográficas da escrita das línguas sinalizadas não estão consolidadas, o que trouxe dificuldades em registrar graficamente as etapas de tradução. As estruturas linguísticas que causaram maiores problemas de tradução foram as que incluíram expressões idiomáticas do português, muitas sem conceitos equivalentes entre o português e a Libras. Foi possível construir um software do WHOQOL-BREF e WHOQOL-DIS em Libras. CONCLUSÕES: O WHOQOL-BREF e o WHOQOL-DIS em Libras possibilitarão que os surdos se expressem autonomamente quanto a sua qualidade de vida, o que permitirá investigar com maior precisão essas questões.

  5. Avaliacao de interacoes medicamentosas potenciais em prescricoes da atencao primaria de Vitoria da Conquista (BA, Brasil

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    Danyllo Fabio Lessa Leao

    2014-01-01

    Full Text Available As interações medicamentosas são fatores de risco para a ocorrência de reações adversas a medicamentos. Este estudo teve o objetivo de avaliar as interações medicamentosas potenciais em prescrições da atenção primária de Vitória da Conquista (BA, visando preencher a lacuna de conhecimento sobre essa temática no Brasil. Foram coletadas informações sobre diversas variáveis de prescrições oriundas da atenção primária e as interações medicamentosas avaliadas a partir dos bancos de dados do Medscape e Micromedex(r. Verificou-se ainda a frequência de polifarmácia e associação desta com a ocorrência de interações medicamentosas. Os resultados mostraram frequência de 48,9% de interações medicamentosas, 74,9% delas de gravidade moderada ou maior, e 8,6% de prescrições em polifarmácia que, em teste qui-quadrado, mostrou associação positiva com ocorrência de interações medicamentosas potenciais (p < 0,001. As prescrições oriundas da atenção primária de Vitória da Conquista (BA apresentaram uma alta frequência de interações medicamentosas, porém faz-se necessária a análise de outros fatores de risco para ocorrência destas nesse nível de atenção à saúde.

  6. Avaliacao da Relacao entre a Doenca Hepatica Nao Gordurosa e CAD utilizando TCMF

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    Duran Efe

    2014-01-01

    Full Text Available Fundamento: Alguns fatores de risco para a aterosclerose são acompanhados pela doença hepática gordurosa não alcoólica (DHGNA. Desejamos usar a tomografia computadorizada multi-fatias (TCMF como a técnica para encontrar relação entre a DHGNA e a doença arterial coronariana (DAC. Objetivo: A relação entre a DHGNA e a DAC foi investigada através de TCMF. Métodos: Um total de 372 indivíduos com ou sem sintomas cardíacos, que foram submetidos à angiografia por TCMF, foram incluídos no estudo. Os pacientes foram divididos em dois grupos, de acordo com a presença da DHGNA. Os segmentos arteriais coronarianos foram avaliados visualmente via angiografia por TCMF. Com base no grau de estenose arterial coronariana, aqueles com placas ausentes ou mínimas foram considerados como normais, enquanto aqueles que apresentavam estenose de menos do que 50% e no mínimo uma placa foram considerados como portadores da doença arterial coronariana não obstrutiva (não-obsDAC. Os pacientes que apresentaram no mínimo uma placa e estenose arterial coronariana de 50% ou mais foram considerados como portadores de doença arterial coronariana obstrutiva (obsDAC. A DHGNA foi determinada de acordo com o protocolo de TCMF, utilizando a densidade hepática. Resultados: De acordo com a densidade hepática, o número de pacientes com doença hepática gordurosa não alcoólica (grupo 1 foi de 204 (149 homens, 54,8% e com fígado normal (grupos 2 foi de 168 (95 homens, 45.2%. Houve 50 (24,5% não-obsDAC e 57 (27,9% casos de obsDAC no Grupo 1, e 39 (23,2% não-obsDAC e 23 (13,7% casos de obsDAC no Grupo 2. Conclusões: O presente estudo utilizando TCMF demonstrou que a frequência da doença arterial coronariana em pacientes com NAFDL foi significativamente superior do que nos pacientes em NAFDL.

  7. Avaliacao da seguranca de diferentes doses de suplementos de acido folico em mulheres do Brasil

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    Quenia dos Santos

    2013-10-01

    Full Text Available OBJETIVO Avaliar a distribuição da ingestão de ácido fólico e a segurança de diferentes doses de suplementos em mulheres em idade reprodutiva. MÉTODOS Foram utilizados dados de consumo a partir de dois dias não consecutivos de registro alimentar de 6.837 mulheres em idade reprodutiva (19 a 40 anos participantes do Inquérito Nacional de Alimentação, módulo da Pesquisa de Orçamentos Familiares 2008-2009. Médias e percentis de consumo habitual de folato natural e ácido fólico foram estimados utilizando o método do National Cancer Institute . Cinco cenários foram simulados somando-se diferentes doses diárias de fortificação (400 mcg, 500 mcg, 600 mcg, 700 mcg e 800 mcg ao ácido fólico oriundo dos alimentos consumidos pelas mulheres. Comparou-se o total de ácido fólico (dieta + suplemento com o nível máximo de ingestão tolerável (UL = 1.000 mcg para definir a dose segura de suplementação. RESULTADOS Mulheres com ingestão habitual de ácido fólico acima do nível máximo de ingestão tolerável foram observadas para doses de suplemento de 800 mcg (7,0% das mulheres. Abaixo desse valor, qualquer dose de suplementação mostrou-se segura. CONCLUSÕES O uso de suplementos de até 700 mcg de ácido fólico mostrou-se seguro.

  8. The major histocompatibility complex genes impact pain response in DA and DA.1U rats.

    Science.gov (United States)

    Guo, Yuan; Yao, Fan-Rong; Cao, Dong-Yuan; Li, Li; Wang, Hui-Sheng; Xie, Wen; Zhao, Yan

    2015-08-01

    Our recent studies have shown that the difference in basal pain sensitivity to mechanical and thermal stimulation between Dark-Agouti (DA) rats and a novel congenic DA.1U rats is major histocompatibility complex (MHC) genes dependent. In the present study, we further used DA and DA.1U rats to investigate the role of MHC genes in formalin-induced pain model by behavioral, electrophysiological and immunohistochemical methods. Behavioral results showed biphasic nociceptive behaviors increased significantly following the intraplantar injection of formalin in the hindpaw of DA and DA.1U rats. The main nociceptive behaviors were lifting and licking, especially in DA rats (PDA rats were significantly higher than those in DA.1U rats in both phases of the formalin test (PDA rats was significantly higher than that of DA.1U rats (PDA was greater than that in DA.1U rats (PDA rats was significantly higher than that in DA.1U rats in the respective experimental group (PDA and DA.1U rats exhibited nociceptive responses in formalin-induced pain model and DA rats were more sensitive to noxious chemical stimulus than DA.1U rats, indicating that MHC genes might contribute to the difference in pain sensitivity. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. The local effect of octreotide on mechanical pain sensitivity is more sensitive in DA rats than DA.1U rats.

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    Yao, Fan-Rong; Wang, Hui-Sheng; Guo, Yuan; Zhao, Yan

    2016-02-01

    A recent study by the authors indicated that major histocompatibility complex (MHC) genes are associated with the differences in basal pain sensitivity and in formalin model between Dark-Agouti (DA) and novel congenic DA.1U rats, which have the same genetic background as DA rats except for the u alleles of MHC. The objective of the present study is to investigate whether there is a difference in the pristane-induced arthritis (PIA) model and local analgesic effect of octreotide (OCT) between DA and DA.1U rats. The hindpaw mechanical withdrawal threshold (MWT) and heat withdrawal latency (HWL) were observed. The C unit firings of the tibial nerve evoked by non-noxious and noxious toe movements were recorded by electrophysiological methods in normal and PIA models in DA and DA.1U rats before and after local OCT administration. The expression of somatostatin receptor 2A (SSTR2A) was observed by immunohistochemistry. The results demonstrate that DA rats have a higher mechanical sensitivity than DA.1U rats after PIA. Local OCT administration significantly elevated MWT in DA rats under normal and PIA sate, but not in DA.1U rats. The electrophysiological experiments showed OCT significantly attenuated the firings of C units evoked by non-noxious and noxious stimulation in DA rats more than those in DA.1U rats both in normal and PIA states. In addition, the expression of SSTR2A in the dorsal horn of the spinal cord was significantly higher in DA than in DA.1U rats. All of the findings suggest a higher local analgesic effect of OCT in DA rats than DA.1U rats, which might be associated with the MHC genes. © 2016 John Wiley & Sons Australia, Ltd.

  10. Leitura rapida do KDIGO 2012: Diretrizes para avaliacao e manuseio da doenca renal cronica na pratica clinica

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    Gianna Mastroianni Kirsztajn

    2014-03-01

    Full Text Available Os autores desta "leitura rápida" apresentam os dados que consideraram mais relevantes na versão 2012 do KDIGO referente à avaliação e manuseio da doença renal crônica. Não se trata da opinião dos autores, mas sim de uma apresentação mais concisa das diretrizes, que podem ser úteis na prática clínica.

  11. Avaliacao da espessura medio-intimal em pacientes com doenca renal cronica nao dialitica: estudo prospectivo de 24 meses

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    Andrea Gaspar Marcos

    2014-03-01

    Full Text Available Introdução: O aumento da espessura média-intimal (EMI avaliada por ultrassom é um preditor de risco cardiovascular na população geral. Porém, em pacientes com doença renal crônica nos estágios iniciais, essa associação ainda não está bem estabelecida. Objetivo: Avaliar a associação EMI com a ocorrência de eventos cardiovasculares e mortalidade em pacientes nos estágios iniciais da doença renal crônica. Métodos: A análise post hoc de uma coorte de pacientes nos estágios 2-4 da DRC. Foram avaliados dados laboratoriais, ultrassom da artéria carótida e tomografia coronariana no início do estudo e a ocorrência de óbito, em seguimento por 24 meses. Resultados: Um total de 117 pacientes (57 ± 11 anos, 61% sexo masculino foram avaliados. A taxa de filtração glomerular foi 36 ± 17 mL/min, 96% dos pacientes eram hipertensos, 23% diabéticos e 27% obesos. Calcificação arterial coronariana esteve presente em 48% dos pacientes, sendo mais prevalente em pacientes nos estágios mais avançados da DRC (p = 0,02. EMI foi 0,6 mm (0,4-0,7 mm. Comparado aos pacientes com EMI < 0,6mm, aqueles com EMI ≥ 0,6 mm eram mais velhos (p = 0,001, apresentavam maior prevalência do sexo masculino (p = 0,001, menor taxa de filtração glomerular (p = 0,01 e maior proporção de pacientes com calcificação (p = 0,001. Não foi observada relação entre a espessura média-intimal e a ocorrência de evento cardiovascular e óbito. Conclusão: A espessura médio-intimal em pacientes DRC se associou à calcificação coronariana, mas não à ocorrência de eventos cardiovasculares e óbito, em um seguimento de 24 meses.

  12. AIDS related thoracic lymphoma: evaluation by computed tomography; Linfoma toracico na sindrome da imunodeficiencia adquirida: avaliacao por tomografia computadorizada

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    Siciliano, Antonio Alexandre de Oliveira [Hospital Universitario Clementino Fraga Filho, Rio de Janeiro, RJ (Brazil). Servico de Radiodiagnostico; Marchiori, Edson [Universidade Federal, Rio de Janeiro, RJ (Brazil). Curso de Pos-graduacao em Radiologia

    2000-02-01

    The authors reviewed five cases of patients with acquired immunodeficiency syndrome (AIDS) related lymphoma to describe the thoracic findings on computed tomography. The patients were followed at Hospital Universitario Clementino Fraga Filho, Hospital Universitario Antonio Pedro and Hospital da Lagoa, from November, 1989 to March 1998. Epidemiological, clinical and pathological data from these patients were quiet variable and pulmonary nodules and masses, hilar and mediastinal lymphadenopathy, and thoracic wall masses were observed. AIDS related lymphomas involving the chest are pleomorphic and most commonly extranodal. (author)

  13. Evaluation of testosterone serum levels in testicular interstitial fluid under thyroxine influence; Avaliacao da testosterona no fluido intersticial testicular sob influencia da tiroxina

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    Silva, Isvania Maria S. da; Pereira, Simey de L.S.; Souza, Grace Mary L.; Carvalho, Elaine F.M.B.; Catanho, Maria Teresa J. de A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia; Silveira, Maria de Fatima G. da [Pernambuco Univ., Recife, PE (Brazil). Dept. de Anatomia; Lima Filho, Guilherme L. [Universidade de Pernambuco (UPE), Nazare da Mata, PE (Brazil). Faculdade de Formacao de Professores

    2000-07-01

    The thyroid hormones possibly exert a reciprocal action between testicular steroids and Sertoli's cells during the premature period. This work aims to evaluate thyroxine effect on testosterone serum levels and in the testicular interstitial fluid (TIF) in rats. Wistar males rats, 22 days old, 80g of body weight, were induced to hyperthyroidism with thyroxine (20{mu}g/kg) in periods of 5, 10, 15 and 20 consecutive days. After the treatment the animals were weighed and sacrificed for blood and testis collection. From the blood serum and from the TIF drained from the testis were performed testes in order to obtain testosterone attached to {sup 125} I with a specific activity of 36,86 MBq/ig. The results have shown a testosterone significant lineal increase in both - serum and TIF - in the group treated with thyroxine as a time function. In the control group, testosterone levels remained low in both serum and TIF dosages. As a result, we were able to verify that the testosterone levels could be modified by thyroxine in serum and TIF. And so, it could affect luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels in hypophysis. (author)

  14. Assessment of natural radioactivity of sands in beaches from Great Vitoria, ES, Brazil; Avaliacao da radioatividade natural em areias das praias da Grande Vitoria, ES

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    Aquino, Reginaldo Ribeiro de

    2010-07-01

    In this work the concentrations of natural radionuclides {sup 226}Ra, {sup 232}Th and {sup 40}K were determined in superficial sand samples for 16 locations throughout the coast of the Great Victory, metropolitan region of the state of Espirito Santo, Southeast of Brazil. The assessed beaches were Manguinhos and Jacaraipe in Serra county, Camburi, Praia do Canto and Curva da Jurema in Vitoria county, Praia da Costa and Itapua in Vila Velha county, Setibao, Setibinha, Praia do Morro, Praia das Castanheiras and Areia Preta in Guarapari county and sand of the Paulo Cesar Vinha Reserve also located in Guarapari county. Three sand samples of each beach were sealed in 100 mL high density polyethylene flasks. After approximately 4 weeks in order to reach secular equilibrium in the {sup 238}U and {sup 2}'3{sup 2}Th series, the samples were measured by high resolution gamma spectrometry and the spectra analyzed with the WinnerGamma software. The self absorption correction was performed for all samples. The {sup 226}Ra concentration was determined from the weighted average concentrations of {sup 214}Pb and {sup 21}'4Bi, the {sup 232}Th concentration was determined from the weighted average concentrations of {sup 228}Ac, {sup 2}'1{sup 2}Pb and {sup 212}Bi and the concentration of {sup 40}K is determined by its single gamma transition of 1460 keV. The radium equivalent concentration and the external hazard index where obtained from the concentrations of {sup 226}Ra, {sup 232}Th and {sup 4}'0K. {sup 226}Ra concentrations show values varying from 3 +- 1 Bq.kg-1 to 738 +- 38 Bq.kg{sup -1}, with the highest values for the central locality of the Camburi beach. {sup 232}Th concentrations show values varying from 7 +- 3 Bq.kg{sup -1} to 7422 +- 526 Bq.kg{sup -1}, with the highest values for Areia Preta beach. {sup 40}K concentrations show values varying from 14 +- 6 Bq.kg{sup -1} to 638 +- 232 Bq.kg{sup -1}, with the highest values for Areia Preta beach

  15. DA1 receptors modulation in rat isolated trachea.

    Science.gov (United States)

    Cabezas, Gloria A; Velasco, Manuel

    2010-01-01

    We have previously demonstrated that low dose of inhaled dopamine (0.5-2 microg kg(-1) min(-1)) induces broncodilatacion in patients with acute asthma attack, suggesting that this dopamine effect is mediated by dopaminergic rather than by adrenergic receptors. To understand better these dopamine effect, rat tracheal smooth muscle was used as a model to evaluate the responses of beta2-, alpha1-, alpha2-adrenergic and DA1 and DA2 dopaminergic antagonists. Tracheal rings from male Sprague-Dawley rats (n = 90) were excised and placed in an organ bath containing modified Krebs-Ringer bicarbonate buffer at 37 degrees C, and gassed with O2 (95%) and CO2 (5%). Contractile responses were recorded with an isometric transducer in a polygraph (Letica, Spain). Contraction was induced by accumulative doses of acetylcholine (0.1, 0.3, 1, 3, 10 mM) or by electric field stimulation (10 Hz at 2 milliseconds), and accumulative doses of dopamine were added to the bath. Low concentration (0.1-0.3 mM) elicited a small initial contraction, followed by a marked relaxation. Cholinergic contraction was completely reversed at 6 mM of dopamine. This biphasic dopaminergic response was not blocked by incubation with beta2-adrenergic antagonist propranolol (0.1 microM), alpha1-antagonist, terazosin (0.1 mM), alpha2-antagonist, yohimbine (0.1 mM), or by DA2 antagonist metoclopramide (1-8 mM); DA1 antagonist SCH23390 (0.1 microM) produced a sustained increase of basal tone but did not block initial dopaminergic contraction and partially inhibited bronchodilator effect of dopamine. Dopaminergic relaxation in rat trachea is mediated by DA1 rather than by DA2 receptors; and adrenergic receptors are not involved in such dopamine-induced response. Finally, DA1 antagonist SCH23390 exerts intrinsic contractile activity on airway smooth muscle that deserves further research.

  16. Evaluation of the natural radioactivity at sands of anomalous regions of Espirito Santo state, Brazil; Avaliacao da radioatividade natural em areias de regioes anomalas do Espirito Santo, Brasil

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    Aquino, Reginaldo Ribeiro de; Pecequilo, Brigitte Roxana Soreanu, E-mail: raquino@ipen.b, E-mail: brigitte@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-10-26

    The natural radioactivity in sands of anomalous regions of the state of Espirito Santo, Brazil, were calculated from the concentration of activity of {sup 40}K, {sup 225}Ra and {sup 232}Th for the places of south and central of Camburi, Curva da Jurema, Ilha do Boi, Setibinha, south and central area of Praia da Areia Preta, denominated Black Spot. The samples were sealed and measured by high resolution gamma spectrometry and after a expectation time pf 30 days, until to reach a radioactive equilibrium

  17. Evaluation of the repeatability of dosimetric parameters of a linear accelerator beam; Avaliacao da repetibilidade de parametros dosimetricos do feixe de um acelerador linear

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    Morelli, Humberto A.S.; Oliveira, Harley F. de; Silva, Maelson do N.; Pavoni, Juliana F., E-mail: h.morelli@hotmail.com [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Servico de Radioterapia

    2016-07-01

    This study aims to evaluate the repeatability of symmetry and flatness of the photon beam of 6 MV linear accelerator Oncor Impression, Siemens, from the Radiotherapy Department of the Hospital das Clinicas da Faculdade de Medicina de Ribeirao Preto - USP. In total they were collected 102 measures, weekly, through two-dimensional detector of ionization chambers, Matrixx Evolution, IBA Dosimetry, connected to a computer with software OmniProI'mrt. The isocenter of the linear accelerator was placed in the center of the sensitive volume of the detector with solid water plates above and below these. Data were collected with 100 UM, with field size 10 x 10 cm². The flatness and symmetry values were calculated by the software used. The first measurement of this work was used as reference, the others were compared with it, taking into account the values of permissible variation for these parameters. The minimum, maximum and average value were analyzed. The literature indicates that the flatness and symmetry values should not vary by more than 2 and 3%, respectively. The results of this work show that the values of these parameters for the linear accelerator study are within the permissible variations in protocols and are therefore able to appropriate clinical use. (author)

  18. Effect of DA-8031, a novel oral compound for premature ejaculation, on male rat sexual behavior.

    Science.gov (United States)

    Kang, Kyung Koo; Sung, Ji Hyun; Kim, Soon Hoe; Lee, Sukhyang

    2014-03-01

    DA-8031 is a potent and selective serotonin transporter inhibitor developed for the treatment of premature ejaculation. The aim of the present study was to investigate the effects of DA-8031 on male sexual behavior in a rat model. Sexual behavior was examined after an acute oral administration of 10, 30 or 100 mg/kg of DA-8031 in copulation studies with female rats. Pharmacokinetic parameters were calculated after oral administration of DA-8031 at a dose level of 30 mg/kg. DA-8031 treatment produced a dose-dependent increase in ejaculation latency time and showed statistical significance at 30 and 100 mg/kg dosage levels compared with the vehicle (P DA-8031 treatment reduced the mean number of ejaculations in a dose-dependent manner. No changes in post-ejaculatory interval, numbers of mounts, intromissions or ejaculations were observed at any dose. In pharmacokinetic study, the blood concentration of DA-8031 peaked at 0.38 ± 0.14 h after oral administration, and then rapidly declined with a half-life of 1.79 ± 0.32 h. Treatment with DA-8031 delays the ejaculation latency time without affecting the initiation of mounting behavior or post-ejaculatory interval in rats. Furthermore, DA-8031 is rapidly absorbed and eliminated after oral administration in rats. These preclinical findings provide a clue for the clinical testing of DA-8031 as an "on-demand" agent for premature ejaculation. © 2013 The Japanese Urological Association.

  19. Effect of DA-9701 on colorectal distension-induced visceral hypersensitivity in a rat model.

    Science.gov (United States)

    Kim, Eun Ran; Min, Byung-Hoon; Lee, Tae Ho; Son, Miwon; Rhee, Poong-Lyul

    2014-07-01

    DA-9701 is a newly developed drug made from the vegetal extracts of Pharbitidis semen and Co-rydalis tuber. The aim of this study was to evaluate the effect of DA-9701 on colorectal distension (CRD)-induced visceral hypersensitivity in a rat model. Male Sprague-Dawley rats were subjected to neonatal colon irritation (CI) using CRD at 1 week after birth (CI group). At 6 weeks after birth, CRD was applied to these rats with a pressure of 20 to 90 mm Hg, and changes in the mean arterial pressure (MAP) were measured at baseline (i.e., without any drug administration) and after the administration of different doses of DA-9701. In the absence of DA-9701, the MAP changes after CRD were significantly higher in the CI group than in the control group at all applied pressures. In the control group, MAP changes after CRD were not significantly affected by the administration of DA-9701. In the CI group, however, the administration of DA-9701 resulted in a significant decrease in MAP changes after CRD. The administration of DA-9701 at a dose of 1.0 mg/kg produced a more significant decrease in MAP changes than the 0.3 mg/kg dose. The administration of DA-9701 resulted in a significant increase in pain threshold in rats with CRD-induced visceral hypersensitivity.

  20. Evaluation of home-made teas efficiency from medicinal plants used on childish diarrhea treatment; Avaliacao da eficacia de chas caseiros de plantas medicinais utilizados no tratamento da diarreia infantil

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Magda Moreira; Silva, Maria Jose de Sousa Ferreira da [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. de Quimica; Vilhena Schayer Sabino, Claudia de; Amaral, Angela Maria [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil)

    1996-03-01

    The objective of this work is to verify whether the home-made teas form Brazilian plants, used for control of childish diarrhea have been efficient reaching the composition recommended by World Health Organizations (WHO). This work has been carried out using the neutron activation analysis and the TRIGA MARK I reactor, the IPR-R1, in the Centro de Desenvolvimento da Tecnologia Nuclear - CDTN. (author). 4 refs., 2 tabs.

  1. System precision assessment ExacTrac 6D® BrainLab of the Hospital das Clinicas da Faculdade de Medicina da USP; Avaliacao da precisao do sistema Exactrac 6D® BrainLab do Hospital das Clinicas da Faculdade de Medicina da USP

    Energy Technology Data Exchange (ETDEWEB)

    Maistro, Carlos E.B., E-mail: carloseduardo.bravinmaistro@gmail.com [Programa de Residencia Multiprofissional em Fisica Medica, Faculdade de Medicina de Sao Paulo, SP (Brazil); Nakandakari, Marcos V.N.; Ribeiro, Victor A.B.; Sales, Camila P. de; Rodrigues, Laura N. [Universidade de Sao Paulo (USP), SP (Brazil). Instituto de Radiologia. Servico de Radioterapia. Hospital das Clinicas

    2015-08-15

    The goal of this study was to evaluate the precision of ExacTrac 6D® Brainlab system, installed at Hospital das Clinicas da Faculdade de Medicina da USP, in frameless radiosurgery treatments. Four sets of tests were performed for different purposes in order to assess the following parameters: the accuracy of location through infrared system; evaluation of the reproducibility of fusion algorithm; evaluation of the X-ray system; and the end-to-end test with the goal of assess the overall accuracy of the system. It was found that the infrared system showed a maximum deviation of 0.5 mm in terms of positioning and the X-ray system showed a precision of 0.15 mm and 0.6°. The reproducibility of fusion algorithms provided a maximum deviation in position which was less than 0.5 mm and 0.5° and the quantitative analysis of the results for end-to-end test showed an overall accuracy of the system better than 0.8 mm. (author)

  2. Whole-genome sequences of DA and F344 rats with different susceptibilities to arthritis, autoimmunity, inflammation and cancer.

    Science.gov (United States)

    Guo, Xiaosen; Brenner, Max; Zhang, Xuemei; Laragione, Teresina; Tai, Shuaishuai; Li, Yanhong; Bu, Junjie; Yin, Ye; Shah, Anish A; Kwan, Kevin; Li, Yingrui; Jun, Wang; Gulko, Pércio S

    2013-08-01

    DA (D-blood group of Palm and Agouti, also known as Dark Agouti) and F344 (Fischer) are two inbred rat strains with differences in several phenotypes, including susceptibility to autoimmune disease models and inflammatory responses. While these strains have been extensively studied, little information is available about the DA and F344 genomes, as only the Brown Norway (BN) and spontaneously hypertensive rat strains have been sequenced to date. Here we report the sequencing of the DA and F344 genomes using next-generation Illumina paired-end read technology and the first de novo assembly of a rat genome. DA and F344 were sequenced with an average depth of 32-fold, covered 98.9% of the BN reference genome, and included 97.97% of known rat ESTs. New sequences could be assigned to 59 million positions with previously unknown data in the BN reference genome. Differences between DA, F344, and BN included 19 million positions in novel scaffolds, 4.09 million single nucleotide polymorphisms (SNPs) (including 1.37 million new SNPs), 458,224 short insertions and deletions, and 58,174 structural variants. Genetic differences between DA, F344, and BN, including high-impact SNPs and short insertions and deletions affecting >2500 genes, are likely to account for most of the phenotypic variation between these strains. The new DA and F344 genome sequencing data should facilitate gene discovery efforts in rat models of human disease.

  3. Whole-Genome Sequences of DA and F344 Rats with Different Susceptibilities to Arthritis, Autoimmunity, Inflammation and Cancer

    Science.gov (United States)

    Guo, Xiaosen; Brenner, Max; Zhang, Xuemei; Laragione, Teresina; Tai, Shuaishuai; Li, Yanhong; Bu, Junjie; Yin, Ye; Shah, Anish A.; Kwan, Kevin; Li, Yingrui; Jun, Wang; Gulko, Pércio S.

    2013-01-01

    DA (D-blood group of Palm and Agouti, also known as Dark Agouti) and F344 (Fischer) are two inbred rat strains with differences in several phenotypes, including susceptibility to autoimmune disease models and inflammatory responses. While these strains have been extensively studied, little information is available about the DA and F344 genomes, as only the Brown Norway (BN) and spontaneously hypertensive rat strains have been sequenced to date. Here we report the sequencing of the DA and F344 genomes using next-generation Illumina paired-end read technology and the first de novo assembly of a rat genome. DA and F344 were sequenced with an average depth of 32-fold, covered 98.9% of the BN reference genome, and included 97.97% of known rat ESTs. New sequences could be assigned to 59 million positions with previously unknown data in the BN reference genome. Differences between DA, F344, and BN included 19 million positions in novel scaffolds, 4.09 million single nucleotide polymorphisms (SNPs) (including 1.37 million new SNPs), 458,224 short insertions and deletions, and 58,174 structural variants. Genetic differences between DA, F344, and BN, including high-impact SNPs and short insertions and deletions affecting >2500 genes, are likely to account for most of the phenotypic variation between these strains. The new DA and F344 genome sequencing data should facilitate gene discovery efforts in rat models of human disease. PMID:23695301

  4. Study of acute hepatotoxicity of Equisetum arvense L. in rats Estudo da hepatotoxicidade aguda da Equisetum arvense L. em ratos

    Directory of Open Access Journals (Sweden)

    Nilo César do Vale Baracho

    2009-12-01

    Full Text Available PURPOSE: To evaluate the acute hepatotoxicity of Equisentum arvense L. in rats. METHODS: Fifty Wistar rats were used, these being divided in four groups, one being the control (receiving only water and the other groups receiving graded doses of Equisentum arvense L. (30, 50, and 100mg/kg respectively for 14 days. Blood samples were obtained to determine TGO, TGP, FA, DHL and GT-gamma activities. After that, hepatic tissue samples were collected for the anatomopathologic analysis. RESULTS: The anatomopathologic exam of the hepatic tissue showed organ with preserved lobular structure. In the same way, there was no significant change in the seric activities of the hepatic enzymes when compared to control group. CONCLUSION: The oral treatment with graded doses of Equisentum arvense L. was not able to produce hepatic changes. Further studies are necessary to evaluate the chronic hepatotoxicity of Equisentum arvense L. in rats.OBJETIVO: Investigar a hepatotoxicidade aguda da Equisetum arvense L. em ratos. MÉTODOS: foram utilizados 50 ratos Wistar, os quais foram divididos em quatro grupos, sendo um controle (recebendo apenas água e os outros grupos recebendo doses crescentes de cavalinha (30, 50 e 100mg/Kg, respectivamente por 14 dias. Foram coletadas amostras de sangue para determinação da atividade sérica de TGO, TGP, FA, DHL e gama-GT. Em seguida, foram obtidas amostras de tecido hepático para análise anatomopatológica. RESULTADOS: O exame anatomopatológico de tecido hepático demonstrou órgão com estrutura lobular preservada. Da mesma forma, não houve alteração significativa na atividade sérica das enzimas hepáticas, quando comparado ao grupo controle. CONCLUSÃO: O tratamento com doses crescentes de Equisetum arvense L., não induziu hepatotoxicidade aguda em ratos. Novos estudos são necessários para avaliar a hepatoxicidade crônica de Equisetum arvense L. em ratos.

  5. Effect of DA-9701 on the Normal Motility and Clonidine-induced Hypomotility of the Gastric Antrum in Rats.

    Science.gov (United States)

    Kang, Je Wook; Han, Dae Kyeong; Kim, Ock Nyun; Lee, Kwang Jae

    2016-04-30

    DA-9701 is a novel prokinetic agent. In the present study, we investigated the effect of DA-9701 on the motility of the gastric antrum in the normal and clonidine-induced hypomotility in an in vivo animal model. A strain gauge force transducer was sutured on the gastric antrum to measure the contractile activity in rats. A total of 28 rats were subclassified into the 4 groups: (1) the placebo group, (2) the DA-9701 group, (3) the placebo group in the clonidine-pretreated rats, and (4) the DA-9701 group in the clonidine-pretreated rats. After the basal recording, either placebo (3% [w/v] hydroxypropylmethyl cellulose) or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. In the clonidinepretreated rats, either placebo or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. Oral administration of DA-9701 did not significantly alter the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the placebo group. The administration of clonidine decreased the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the administration of placebo. This reduction of the antral motility by the administration of clonidine was not observed in the clonidine-pretreated DA-9701 group. The percentage of the motility index in the postprandial period was significantly greater in the clonidine-pretreated DA-9701 group, compared with the clonidine-pretreated placebo group. DA-9701 improves the hypomotility of the gastric antrum induced by clonidine, suggesting its gastroprokinetic effect in the pathologic condition.

  6. Concentration change of DA, DOPAC, Glu and GABA in brain tissues in schizophrenia developmental model rats induced by MK-801.

    Science.gov (United States)

    Liu, Yong; Tang, Yamei; Pu, Weidan; Zhang, Xianghui; Zhao, Jingping

    2011-08-01

    To explore the related neurobiochemical mechanism by comparing the concentration change of dopamine (DA), dihydroxy-phenyl acetic acid (DOPAC), glutamate (Glu), and γ-aminobutyric acid (GABA) in the brain tissues in schizophrenia (SZ) developmental model rats and chronic medication model rats. A total of 60 neonatal male Spragur-Dawley (SD) rats were randomly assigned to 3 groups at the postnatal day 6: an SZ developmental rat model group (subcutaneous injection with MK-801 at the postnatal day 7-10, 0.1 mg/kg, Bid), a chronic medication model group (intraperitoneal injection at the postnatal day 47-60, 0.2 mg/kg,Qd), and a normal control group (injection with 0.9% normal saline during the corresponding periods). DA, DOPAC, Glu, and GABA of the tissue homogenate from the medial prefrontal cortex (mPFC) and hippocampus were examined with Coularray electrochemic detection by high performance liquid chromatogram technique. The utilization rate of DA and Glu was calculated. Compared with the normal control group, the concentration of DA and DOPAC in the mPFC and the hippocampus in the SZ developmental model group significantly decreased (PGABA concentration and Glu utilization rate in the mPFC also decreased (PGABA system decrease in the mPFC and the DA system function reduces in the hippocampus of SZ developmental rats.

  7. Thermal hydraulic evaluation for an experimental facility to investigate pressurized thermal shock (PTS) in CDTN/CNEN; Avaliacao termo-hidraulica da montagem experimental de choque termico pressurizado do CDTN/CNEN

    Energy Technology Data Exchange (ETDEWEB)

    Palmieri, Elcio T.; Navarro, Moyses A.; Aronne, Ivam D.; Terra, Jose L. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil)

    2002-07-01

    The goal of the work presented in this paper is to provide necessary thermal hydraulics information to the design of an experimental installation to investigate the Pressurized Thermal Shock (PTS) to be implemented at Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN). The envisaged installation has a test section that represents, in a small scale, a pressure vessel of a nuclear reactor. This test section will be heated and then exposed to a PTS in order to evaluate the appearance and development of cracks. To verify the behavior of the temperatures of the pressure vessel after a sudden flood through the annulus, calculations were made using the RELAP5/MOD 3.2.2 gamma code. Different outer radiuses were studied for the annular region. The results showed that the smaller annulus spacing (20 mm) anticipates the wetting of the surface and produces a higher cooling of the external surface, which stays completely wet for a longer time. (author)

  8. Hormonal evaluation of T{sub 4} and T{sub 3} through radioimmunoassay in younglings of rats with hypothyroidism; Avaliacao hormonal de T{sub 4} e T{sub 3} atraves de radioimunoensaio em filhotes de ratas hipotireoideas

    Energy Technology Data Exchange (ETDEWEB)

    Silveira, M.F.G. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia; Silva, I.M.S.; Pereira, S.S.L.; Souza, G.M.L.; Carvalho, E.F.M.B.; Cavalcante, C.V.G. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Anatomia; Lima Filho, G.L.; Catanho, M.T.J.A. [Faculdade de Formacao de Professores de Nazare da Mata, PE (Brazil). Dept. de Ciencias Naturais

    2000-07-01

    The onset of fetal thyroid function occurs about 17-18 days after conception in the rat. The maternal hypothyroidism which occurs during gestation provokes alteration in the rat after birth. Due to this alteration, we decided to analyze the hormonal modification in the newborn rats. The hypothyroidism was induced in normal dams, which were being treated for 7 days with MMI (in the concentration of 0,03% in drinking water) before mating. Another dam group which was submitted to an induction of hypothyroidism maintained the treatment with MMI for 13 days during gestation. The hormones were assessed by radioimmunoassay technique. It was seen that the rats which were born from hypothyroid dams suffered alterations on its T{sub 4} and T{sub 3} hormone levels concerning to 10, 30 and 60 days after birth. There was also modifications on their weight and size. The growth is affected throughout post-natal life by thyroid hormones, which has a facilitator influence on growth hormone economy, as opposed to the inhibitory effects on TSH economy. The administration of MMI bars the fetal thyroid gland function, causing a decrease of both T{sub 4} and T{sub 3} levels, even after the birth, indicating that the maternal hypothyroidism influences on the post-natal life of the rat. (author)

  9. Evaluation of solubility in simulated lung fluid of metals present in the sludge from a metallurgical industry to produce metallic zinc; Avaliacao da solubilidade em liquido pulmonar simulado dos metais presentes no rejeito gerado por uma industria metalurgica de zinco

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Rosilda Maria Gomes de

    2012-07-01

    The objective of this study was to determine the solubility parameters (rapid and slow dissolution rates, rapid and slow dissolution fractions) metal particles present in a pile of sludge accumulated under exposure to weathering from the Cia Mercantil Inga, located at the Ilha da Madeira, Sepetiba Bay, Rio de Janeiro. Plant samples collected in the neighboring of the pile and bioindicators placed in the region and collected after some months indicated that the inhabitants of Ilha da Madeira have been exposed to trace elements such zinc, cadmium, mercury and lead, produced during the processing of zinc minerals (hemimorphite - Zn{sub 4}(OH){sub 2}Si{sub 2}O{sub 7}.H{sub 2}O, and willemite - Zn{sub 2}SiO{sub 4}). A static dissolution test in vitro was used to determine the solubility parameters using a simulated lung fluid (SLF), on a time basis ranging from 10 min to 1 year. The metal concentrations in the sludge samples and in the SLF were determined using Particle Induced X-rays Emission (PIXE). In conclusion, this study confirms the harmful effects on the neighboring population of the airborne particles containing these metals that came from the sludge. The solubility parameters obtained for Zn, Cd, Cr, Ni and Mn present in the rapid dissolution fraction in SLF were 0.945; 0.473; 0.226; 0.300 and 0.497, respectively, and the corresponding times for half life of dissolution of the rapid fraction were f{sub r} = 2.082 days; f{sub r} = 0.09 days; f{sub r} = 0.37 days; f{sub r} = 0.332 days ad f{sub r} = 0.99 days; for the slow dissolution fraction times were f{sub r} = 146.95 days; f{sub r} = 63 days; f{sub r} = 86.64 days; f{sub r} = 79.66 days and f{sub r} = 59.84 days. These values indicate that these metals present a moderate absorption level in SLF, and may be classified as M type, according to the International Commission on Radiological Protection (ICRP). The use of solubility parameters allowed a better description of the kinetic behaviour of the sludge in

  10. Evaluation of porous vitreous carbon or silicon implants by radiology in rat's skull; Avaliacao radiologica de implantes de carbono vitreo poroso ou silicone em cranio de ratos

    Energy Technology Data Exchange (ETDEWEB)

    Vaccari-Mazzetti, Marcelo Paulo; Kobata, Celio Toshiro [Lusiada University of Santos, SP (Brazil). Hospital Defeitos da Face. Dept. of Surgery]. E-mail: mmgvaccari@ig.com.br; Fabiani, Paulo [Lusiada University of Santos, SP (Brazil). Dept. of Radiology; Martins, Dulce Maria Fonseca Soares [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. of Surgery. Div. of Plastic Surgery; Gomes, Paulo de Oliveira [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. of Surgery. Div. of Operatory Technique and Experimental Surgery; Martins, Jose Luiz [Universidade Federal de Sao Paulo (UNIFESP/EPM), SP (Brazil). Dept. of Surgery. Div. of Pediatric Surgery

    2008-07-01

    Purpose: Evaluate by CT the use of porous vitreous carbon (PVC) and silicon (S) implants as the replacement bone in the craniofacial skeleton of rats. Methods: 40 rats divided in: Group A (n=20) PVC submitted to the implant of a fragment in skull. After the euthanasia, the animals were divided into two subgroups: A I: 10 animals, studied in the 7th postoperative day (P.O) and AII: 10 animals, studied in the 28th P.O. In group B, S, 20 rats were submitted to S implant in the skull. All other steps were identical to group A, with designation of subgroups BI and BII. CT with beams in axial cuts of 1 mm thickness to obtain 3-D information It was used Hounsfield scale for evaluate the radio density of the implant. They were used non parametric tests to analyze the results. Results: The 7th PO boss remained in the two groups, but for 28th PO, observed reduction in the volume of the implant in Group A, not observed in group B. CT studies noticed different radio densities around all of S prostheses (pseudo capsule), that do not appeared in CPV implants. The S has remained unchanged in the CT, but the CPV has had a modification in its radio density (p{<=}0,05), in all implants. Conclusion: In CT evaluation the implants of CPV have greater deformation that the S, which makes them not suitable for replacement of membranous bone in the rat skull. (author)

  11. An assessment of ninth round; Nona: uma avaliacao da rodada

    Energy Technology Data Exchange (ETDEWEB)

    Assumpcao, Eduardo; Andrade, Leila; Fontana, Raphaela [Agencia Nacional do Petroleo, Gas Natural e Biocombustiveis (ANP), Rio de Janeiro, RJ (Brazil)

    2008-07-01

    Accomplished on November, 27{sup th}, 2007, 271 exploratory blocks were offered in Round 9, distributed within 14 sectors, totalling an area of 73 thousand km{sup 2}. The following basins were included: Campos, Espirito Santo, Para- Maranhao, Parnaiba, Pernambuco-Paraiba, Potiguar, Santos, Reconcavo and Rio do Peixe. From the original lot of 67 companies qualified (31 Brazilian and 36 of foreign origin), 42 offered bids individually or in partnerships. 117 blocks were allocated to 24 winning operator companies. Other 12 enterprises won acreage as non-operator participants of joint bids. A record of R$ 2,1 billion were offered as signature bonuses along with 169.436 units of the so called minimum exploratory programs. These units may be converted to an estimated R$ 1,4 billion. The objective of this paper is to evaluate the results obtained in Round 9, analyzing collected data through three different perspectives of aggregate results: bidding companies, offered areas and exploratory models. (author)

  12. Efeitos da betametasona sobre os fetos e placentas da rata albina Effects of betamethasone on the fetuses and placentas of female albino rats

    Directory of Open Access Journals (Sweden)

    Eduardo de Souza

    2001-03-01

    Full Text Available Objetivos: analisar os efeitos da betametasona sobre o binômio materno-fetal da rata albina. Métodos: utilizamos 60 ratas albinas prenhes divididas ao acaso em três grupos numericamente iguais. As ratas do grupo I foram tratadas com betametasona na dose de 1 mg/kg de peso em 0,5 ml de água destilada, por via intramuscular no 11º, 12º, 18º e 19º dia de prenhez; as do grupo II receberam 0,5 ml de água destilada, por via intramuscular no 11º, 12º, 18º e 19º dia de prenhez, e as do grupo III não receberam qualquer fármaco ou veículo. O ganho de peso das matrizes foi avaliado nos dias 0, 7, 14 e 20 de prenhez, sendo que no 20º dia todos os animais foram sacrificados por decapitação. Foram quantificados o número de implantações, de reabsorções, de fetos, de placentas, de malformações maiores, de mortalidade materna e fetal, assim como o peso dos fetos e das placentas. Resultados: nossos resultados revelaram que as matrizes tratadas com betametasona apresentaram menor ganho de peso. Quanto aos fetos e as placentas do grupo tratado, observamos que os pesos foram inferiores aos dos outros grupos. A média de peso dos fetos foi de 3,20 g contra 3,75 g no grupo controle. A média de peso das placentas foi de 0,36 g no grupo tratado com betametasona contra 0,48 g no grupo controle. Todas estas diferenças foram estatisticamente significantes. Conclusões: a betametasona apresenta efeito negativo sobre o ganho de peso das matrizes, fetos e placentas, quando administrada de forma repetitiva a partir da segunda metade da prenhez.Purpose: to analyze the effect of betamethasone on the pregnancy of rats. Methods: thirty pregnant rats were divided into three groups of ten animals each. Group I -- the animals received betamethasone IM (1 mg/kg body weight, in 0.5 ml distilled water on the 11th, 12th, 18th and 19th day of pregnancy. Group II -- the rats received distilled water (0.5 ml IM on the 11th, 12th, 18th and 19th day of pregnancy

  13. Effect of Da-Cheng-Qi Decoction on Pancreatitis-Associated Intestinal Dysmotility in Patients and in Rat Models

    Directory of Open Access Journals (Sweden)

    Jianlei Zhao

    2015-01-01

    Full Text Available The impairment of intestinal motility and related infectious complications are the predominant clinical phenomenon in patients with severe acute pancreatitis (SAP. We aimed to investigate the effects of Da-Cheng-Qi decoction (DCQD on the gastrointestinal injury in SAP patients and the potential mechanism involved in rats. DCQD was enema administered to 70 patients for 7 days in West China Hospital. Mortality and organ failure during admission were observed and blood samples for laboratory analysis were collected. We also experimentally examined plasma inflammatory cytokines in rat serum and carried the morphometric studies of the gut. Intestinal propulsion index and serum and tissue vasoactive intestinal peptide (VIP were also detected. Though DCQD did not affect the overall incidence of organ failure, it shortened the average time of paralytic intestinal obstruction and decreased the morbidity of infectious complications in patients with SAP. Compared with untreated rats, the DCQD lowered the levels of proinflammatory cytokine and decreased the mean pathological intestinal lesion scores. The VIP level in intestinal tissue or serum in DCQD group was obviously lowered and intestinal propulsion index was significantly improved. In conclusion, DCQD has good effect on pancreatitis-associated intestinal dysmotility in patients and in rat models.

  14. Decreased Hippocampal 5-HT and DA Levels Following Sub-Chronic Exposure to Noise Stress: Impairment in both Spatial and Recognition Memory in Male Rats.

    Science.gov (United States)

    Haider, Saida; Naqvi, Fizza; Batool, Zehra; Tabassum, Saiqa; Perveen, Tahira; Saleem, Sadia; Haleem, Darakhshan Jabeen

    2012-01-01

    Mankind is exposed to a number of stressors, and among them noise is one which can cause intense stress. High levels of background noise can severely impair one's ability to concentrate. The present study was aimed to investigate the effect of sub-chronic noise stress on cognitive behavior and hippocampal monoamine levels in male rats. The study was performed on 12 male Wistar rats, divided into two groups; the control and noise-exposed. The rats in the test group were subjected to noise stress, 4h daily for 15 days. Cognitive testing was performed by the Elevated Plus Maze test (EPM) and Novel Object Recognition test (NOR). HPLC-EC was used to determine hippocampal monoamine levels and their metabolites. The data obtained revealed a significant decrease in hippocampal serotonin (5-hydroxytryptamine; 5-HT) and dopamine (DA) levels, whereas turnover ratios of 5-HT and DA were significantly increased compared to the controls. Rats exposed to noise exhibited a significant decrement in spatial memory. A significantly decreased recognition index of rats exposed to noise as compared to the control was also observed in the NOR test. Results of the present findings suggest the role of decreased hippocampal 5-HT and DA in the impairment of cognitive function following noise exposure.

  15. Avaliação da toxicidade oral subcrônica da bixina para ratos Oral toxicity assessment of annatto in rats

    Directory of Open Access Journals (Sweden)

    Ana Rita Pedreira Lapa Bautista

    2004-06-01

    Full Text Available A bixina em pó (30% de bixina, proveniente das sementes de urucum (Bixa orellana L., foi administrada, por gavagem, a ratos Wistar, 10 animais de cada sexo, na concentração de 0,01±0,006% de bixina/dia, em óleo de milho, cinco dias por semana, durante 13 semanas, com o objetivo de verificar a toxicidade da substância-teste para essa espécie animal. A grupos controle (10 animais por sexo, foi administrado óleo de milho, para comparação. Durante o período de exposição, foram registrados o peso absoluto corpóreo, o ganho de peso, o consumo de ração e a eficiência alimentar, bem como realizadas as avaliações clínica e oftalmoscópica. Antes da eutanásia, os animais foram anestesiados (éter etílico e submetidos a exames hematológicos de rotina e bioquímicos (glicose, creatinina, colesterol total, triglicérides, asparagina transaminase e g-glutamil transaminase. Durante o exame necroscópico, fígado, rins, baço, adrenais e testículos foram excisados e pesados. O estudo histológico foi realizado em amostras de fígado e rins dos animais expostos e respectivos controles. A análise estatística dos parâmetros de peso, hematológicos e bioquímicos mostrou algumas diferenças significativas entre os grupos teste e controle, as quais não parecem estar relacionadas à exposição. Não foram observadas alterações clínicas, comportamentais, necroscópicas e histológicas. Nas condições do estudo, a bixina não produziu efeitos tóxicos nos animais expostos.The aim of the investigation was to determine the possible health hazards of bixin (30% from annatto (Bixa orellana L. origin to rats. A concentration of 0.01±0.006%/day of bixin in corn oil was administered to 20 Wistar rats (10 per sex, through the oral route (gavage over a period of 13 weeks. A group of untreated animal (10 per sex acting as a control (corn oil was used for comparision. Body weight, body weight gain, feed consumption and feed efficiency were

  16. Protective effect of DA-9401 in finasteride-induced apoptosis in rat testis: inositol requiring kinase 1 and c-Jun N-terminal kinase pathway

    Directory of Open Access Journals (Sweden)

    Soni KK

    2017-10-01

    Full Text Available Kiran Kumar Soni,1,* Yu Seob Shin,1,* Bo Ram Choi,1 Keshab Kumar Karna,1 Hye Kyung Kim,2 Sung Won Lee,3 Chul Young Kim,4 Jong Kwan Park1 1Department of Urology, Chonbuk National University and Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute and Clinical Trial Center of Medical Device of Chonbuk National University, Jeonju, 2College of Pharmacy, Kyungsung University, Busan, 3Department of Urology, Samsung Medical Center, Samsung Biomedical Research Institute, Sungkyunkwan University Medical School, Seoul, 4College of Pharmacy, Hangyang University, Ansan, Republic of Korea *These authors contributed equally to this work Abstract: Finasteride is used to treat male pattern baldness and benign prostatic hyperplasia. This study investigated the toxicity of finasteride and recovery by DA-9401 using Sprague Dawley (SD rats. Forty adult male SD rats were assigned to four groups: control (CTR, finasteride 1 mg/kg/day (F, finasteride 1 mg/kg + DA-9401 100 mg/kg/day (F + DA 100 and finasteride 1 mg/kg + DA-9401 200 mg/kg/day (F + DA 200. Treatments were by oral delivery once daily for 90 consecutive days. The gross anatomical parameters assessed included: genital organ weight; vas deferens sperm count and sperm motility; testosterone, dihydrotestosterone (DHT and malondialdehyde levels; and histological and terminal deoxynucleotidyl transferase enzyme mediated dUTP nick-end labeling (TUNEL staining of testis for spermatogenic cell density, Johnsen’s score and apoptosis. Testicular tissue was also used for evaluating endoplasmic reticulum (ER stress and apoptotic proteins. Epididymis weight, seminal vesicle weight, prostate weight, penile weight and vas deferens sperm motility showed significant differences between the F group and the CTR, F + DA 100 and F + DA 200 groups. There was no significant change in the testosterone level. DHT level decreased significantly in the F group compared with the CTR

  17. Da-Chaihu-Tang alters the pharmacokinetics of nifedipine in rats and a treatment regimen to avoid this.

    Science.gov (United States)

    He, Ju-Xiu; Ohno, Kenji; Tang, Jun; Hattori, Masao; Tani, Tadato; Akao, Teruaki

    2014-11-01

    To investigate the influence of co-administrated Da-Chaihu-Tang (DCT; a traditional Chinese formulation) on the pharmacokinetics of nifedipine, as well as the safe optimal dosing interval to avoid the adverse interactions. A single dose of DCT was administered with nifedipine simultaneously, 2 h before, 30 min before or 30 min after nifedipine administration. Pharmacokinetics of nifedipine with or without DCT were compared. The influences of DCT on nifedipine intestinal mucosal and hepatic metabolism were studied by using rat in-vitro everted jejunal sac model and hepatic microsomes. A simultaneous co-administration of DCT significantly increased the area under concentration-time curve from time zero to infinity (AUC0-inf ) of nifedipine. In-vitro mechanism investigations revealed that DCT inhibited both the intestinal and the hepatic metabolism of nifedipine. Further study on the optimal dosing interval for nifedipine and DCT revealed that administration of DCT 30 min before or after nifedipine did not significantly change the AUC of nifedipine. The bioavailability of nifedipine is significantly increased by a simultaneous oral co-administration of DCT. This increase is caused by the inhibitory effect of DCT on both the intestinal mucosal and the hepatic metabolism of nifedipine. The dose interval between DCT and nifedipine needs to be set for over 30 min to avoid such drug-drug interactions. © 2014 Royal Pharmaceutical Society.

  18. Ultrastructure of cranial nerves of rats inoculated with rabies virus Ultraestrutura de nervos cranianos de ratos inoculados com o vírus da raiva

    Directory of Open Access Journals (Sweden)

    Guilberto Minguetti

    1997-01-01

    Full Text Available The V and VII cranial nerves of rats inoculated with rabies virus were studied by electron microscopy. The results were compared with the same cranial nerves of rats inoculated with rabies virus but vaccinated against the disease. The findings are those of axonal degeneration and intense demyelination of the nerves of the group of rats not vaccinated. The vaccinated rats showed some ultrastructural irrelevant alterations when compared with the other group. The degree of ultrastructural alterations found in the group of rats not vaccinated suggests that in rabies severe damage of the cranial nerves occurs and that this may be closely related to the clinical picture of the disease (hydrophobia. Furthermore, as far as the authors know, this has not been considered in the classic descriptions of rabies and it is possible that an immunologic process may take part in the demyelination observed in the present study.Os autores estudaram o quinto e o sétimo nervos cranianos de ratos inoculados com o vírus da raiva. Os resultados foram comparados com os mesmos nervos cranianos de ratos inoculados com o vírus da raiva, porém vacinados contra a doença. Os achados no grupo não vacinado foram de degeneração axonal e intensa desmielinização dos nervos examinados. No grupo vacinado foram encontrados apenas discretas alterações da mielina, sem relevância do ponto de vista patológico. As grandes alterações ultraestruturais encontradas no grupo de ratos não vacinados sugerem que na raiva ocorram acentuadas alterações nos nervos cranianos e que tais alterações devem estar intimamente relacionadas ao quadro clínico da doença (hidrofobia. Além disso, é possível que tais alterações estejam associadas a um processo imunológico responsável também por acometimento sistêmico dos nervos periféricos.

  19. Efeito do extrato da casca de Syzygium cumini sobre a atividade da acetilcolinesterase em ratos normais e diabéticos Syzygium cumini bark extract effect on acetylcholinesterase activity in normal and diabetic rats

    Directory of Open Access Journals (Sweden)

    Cinthia Melazzo Mazzanti

    2004-06-01

    Full Text Available Este estudo verificou a eficiência do extrato etanólico da casca de Syzygium cumini sobre o sistema colinérgico de ratos normais e diabéticos induzidos com aloxano. Os animais foram divididos em grupo controle (C, tratado com Syzygium cumini (TS, diabético (D e diabético tratado com Syzygium cumini (DS. A atividade da acetilcolinesterase (AChE foi analisada nas seguintes estruturas cerebrais: cerebelo, córtex, estriado e hipocampo. O extrato etanólico da casca de Syzygium cumini na dose de 1g.kg-1 foi administrado diariamente por um período de trinta dias. Foi verificado após este período que o extrato inibiu a atividade da AChE no cerebelo e córtex cerebral dos ratos do grupo DS (PThe present study verified the efficiency of the bark ethanol extract of Syzygium cumini on the cholinergic system of normal and alloxan induced diabetic rats. Thirty-nine female rats were divided in control (C, treated with Syzygium cumini (TS, diabetic (D and diabetic treated with Syzygium cumini (DS. The activity of acetylcholinesterase (AChE was analyzed in the following cerebral structures: cerebellum, cortex, striatum and hippocampus. The extract of the bark of Syzygium cumini in the dose of 1g.kg-1 was administered orally daily for a period of thirty days. After this period the extract inhibited the activity of the AChE in the cerebellum and cerebral cortex of the rats in the DS group (P<0.05 as, compared to TS. In the striatum there was a significant increase in the activity of the AChE in rats of the TS group (P<0.01 when compared to the C group, and in the hippocampus there was no significant variation. These results indicate that the bark extract of "Jambolão"has an inhibitory effect on AChE in the cerebellum and cerebral cortex and an stimulatory effect on striatum, indicating a possible alteration in the functionality of the cholinergic system in such cerebral structures.

  20. 4-[[sup 123]I]iodospiperone as a ligand for dopamine DA receptors: in vitro and in vivo experiments in a rat model

    Energy Technology Data Exchange (ETDEWEB)

    Krogt, J.A. van der; Valkenburg, C F.M. van; Pauwels, E K.J.; Buruma, O J.S. [Rijksuniversiteit Leiden (Netherlands); Reiffers, S [Hospital De Weezenlanden, Zwolle (Netherlands); Doremalen, P.A.P.M. van; Wijnhoven, G [Cygne, Eindhoven (Netherlands)

    1992-10-01

    Radioiodinated spiperone is of interest for dopamine (DA) receptor studies in the living human brain by single photon emission computed tomography (SPECT). Simulated by data obtained with [[sup 11]C]-N-methyl-spiperone, we synthesized 4-[[sup 123]I]iodospiperone and investigated the in vitro binding characteristics of this ligand to the striatal membrane of the rat and the in vivo distribution over various rat brain regions. The in vitro binding experiments showed that this radioligand displays about 10 times less affinity for the DA receptor than spiperone and specific binding, as shown with [[sup 3]H]spiperone, was not observed. Displacement by butaclamol was not observed. The in vivo studies demonstrated that both 4-[[sup 123]I]iodospiperone and [[sup 3]H]spiperone concentrate in striatal tissue, respectively, 1.9 and 3.5 times as high as in cerebellar tissue. Haloperidol pretreatment largely prevented this accumulation. In view of the obtained target-to-non-target ratios we believe, however, that this accumulation in brain areas rich DA-receptors does not offer prospects for clinical receptor imaging with SPECT. (Author).

  1. [A Paired Case Controlled Study Comparing the Short-term Outcomes of Da Vinci RATS and VATS Approach for Non-small Cell Lung Cancer].

    Science.gov (United States)

    Dai, Feng; Xu, Shiguang; Xu, Wei; Ding, Renquan; Liu, Bo; Meng, Hao; Kang, Yunteng; Meng, Xiangrui; Lin, Jie; Wang, Shumin

    2018-03-20

    Da Vinci Surgical System is one of the greatest inventions of the 20th century, which represents the development direction of the precise minimally invasive surgical techniques, the aim of this study was to comparing the short-term outcomes between da Vinci robot-assisted lobectomy and video-assisted thoracic surgery (VATS) lobectomy for non-small cell lung cancer. 45 pairs of non-small cell lung cancer patients underwent pulmonary lobectomy with da Vinci Robotic assisted thoracoscopic (RATS) and VATS approach during the same period from January 2014 to January 2017. The operative time, estimated blood loss (EBL), total number and total groups of dissected lymph nodes, postoperative duration of drainage, the first day volume of drainage, total volume of drainage were compared. No perioperative death and convertion to thoracotomy occured in both groups. There were significant difference between RATS group and VATS group in EBL [(50.30±32.33) mL vs (208.60±132.63) mL], the first day volume of drainage [(275.00±145.42) mL vs (347.60±125.80) mL], the dissected total number [(22.67±9.67) vs (15.51±5.41)] and total team [(6.31±1.43) vs (4.91±1.04)] of lymph node. There were no significant difference in other outcomes. RATS is safe and effective and took better short-outcomes than VATS in non-small cell lung cancer.

  2. Decision theory on the quality evaluation of medical images; A teoria da decisao na avaliacao da qualidade da imagem medica

    Energy Technology Data Exchange (ETDEWEB)

    Lessa, Patricia Silva

    2001-10-01

    The problem of quality has been a constant issue in every organization.One is always seeking to produce more, to do it at a lower cost, and to do it with better quality. However, in this country, there is no radiographic film quality control system for radiographic services. The tittle that actually gets done is essentially ad hoc and superficial. The implications of this gap, along with some other shortcomings that exist in process as a whole (the state of the x-ray equipment, the adequate to use in order to obtain a radiography, the quality of the film, the processing of the film, the brightness and homogeneity of the viewing boxes, the ability of the radiologist), have a very negative impact on the quality of the medical image, and, as result, to the quality of the medical diagnosis and therapy. It frequently happens that many radiographs have to be repeated, which leads to an increase of the patient's exposure to radiation, as well as of the cost of the procedure for the patient. Low quality radiographs that are not repeated greatly increase the probability of a wrong diagnosis, and consequently, of inadequate therapeutical procedures, thus producing increased incidence of bad outcomes and higher costs. The paradigm proposed in order to establish a system for the measurement of the image's quality is Decision Theory. The problem of the assessment of the image is studied by proposing a Decision Theory approach. The review of the literature reveals a great concern with the quality of the image, along with an absence of an adequate paradigm and several essentially empirical procedures. Image parameters are developed in order to formalize the problem in terms of Decision Theory, and various aspects of image digitalisation are exposed. Finally, a solution is presented, including a protocol for quality control. (author)

  3. Exploration of D2 receptors and content of DA of striatum in hemi-parkinsonism model rats before and after madopar treatment

    International Nuclear Information System (INIS)

    Lin Yansong; Lin Xiangtong

    1998-01-01

    125 I-IBZM autoradiographic analysis, HPLC-ECD were used to study the relationship between D 2 receptors and the content of DA, HVA, DOPAC in striatum of hemi-parkinsonism model rats before and after Madopar treatment. After Madopar treatment, the striatum/cerebellum 125 I-IBZM uptake ratio of lesioned side was 7.23 +- 0.67, showed 17.22 +- 3.94% increasing as compared to the contralateral side, the increasing were significantly declined compared with the pretreatment group and control group (P 2 receptors

  4. Effect of interaction of heavy metals on (Na+-K+) ATPase and uptake of 3H-DA and 3H-NA in rat brain synaptosomes

    International Nuclear Information System (INIS)

    Chandra, S.V.; Murthy, R.C.; Husain, T.; Bansal, S.K.

    1984-01-01

    The effect of interaction of Mn 2+ , Pb 2+ and CD 2+ on (Na + -K + ) ATPase and uptake of labelled dopamine ( 3 H-DA) and labelled noradrenaline ( 3 H-NA) were studied in vitro in rat brain synaptosomes. The inhibition of (Na + -K + )ATPase by Pb 2+ + Cd 2+ alone was concentration dependent, however, Mn 2+ had almost no effect on the activity of this enzyme. Interaction of Cd 2+ with either Pb 2+ or Mn 2+ was almost powerful in inhibiting the activity of synaptosomal transport ATPase. Lower concentrations of Pb 2+ increased while higher concentrations inhibited synaptosomal uptake of 3 H-DA and 3 H-NA. Lower concentrations of CD 2+ increased the uptake of 3 H-DA while at concentrations of 100 μM, the uptake was inhibited, this metal had strong inhibitory effect on the uptake of 3 H-NA. Mn 2+ had inhibited the uptake of labelled amines. Interaction of Mn 2+ with Pb 2+ or Cd 2+ produced inhibition on the uptake of 3 H-DA and 3 H-NA. The results of the uptake of biogenic amines in the presence of metal ions apparently had no correlation with the activity og (Na + -K + ) ATPase which is involved in the active transport of cations across cell membranes. (author)

  5. Activity evaluation from different native or irradiated with {sup 60} Co gamma rays snake venoms and their inhibitory effect on Leishmania (Leishmania) amazonensis; Avaliacao da atividade de diferentes venenos de serpentes, nativos ou irradiados, com radiacao gama de {sup 60} Co, quanto ao poder inibitorio do crescimento de Leishmania (Leishmania) amazonensis

    Energy Technology Data Exchange (ETDEWEB)

    Lourenco, Cecilia de Oliveira

    2000-07-01

    Cutaneous leishmaniasis is a disease, caused by Leishmania parasites, that occurs frequently in tropical and sub-tropical regions of the world. Skin lesions that could results in disfiguring aspect characterize it. The treatment is based on few drugs as antimony salts or pentamidine that are toxic with increasing resistance by the parasite. Alternative forms of disease treatment are in constant search, including natural components as snake venoms. Previous studies demonstrate that some components of snake venoms have an inhibitory effect against those parasites, including Leishmania species. Although snake venoms presented high toxicity, several methods have been described to detoxify most or some of their toxic components, with favorable results by the use of gamma irradiation. In this report we tested several native and irradiated snake venoms for inhibitory effect against Leishmania (Leishmania) amazonensis parasite and LLCMK{sub 2} mammalian cells, with enzymatic tests and electrophoresis. There are significant activity in Acanthophis antarcticus, Agkistrodon bilineatus, Bothrops moojeni, Bothrops jararaca, Hoplocephalus stephensi, Naja melanoleuca, Naja mossambica, Pseudechis australis, Pseudechis colletti, Pseudechis guttatus and Pseudechis porphyriacus, venom being inactive Pseudonaja textilis, Notechis ater niger, Notechis scutatus. Oxyuranus microlepidotus and Oxyuranus scutellatus venoms. After 2 KGy of {sup 60}Co irradiation most venom loses significantly their activity. Venoms with antileishmanial activity presented L-amino acid oxidase (L-AO) activity and showed common protein with a molecular weight about 60kDa in SDS-PAGE. These results indicate that L-AO activity in those venoms are probably related with antileishmanial effect. (author)

  6. Comparison of rimonabant and sibutramine treatment effects on food compulsion in rats Comparação do efeito do rimonabanto e da sibutramina no tratamento da compulsão alimentar em ratos

    Directory of Open Access Journals (Sweden)

    Honório Sampaio Menezes

    2009-12-01

    Full Text Available PURPOSE: To compare the therapeutic effect of rimonabant, a new drug which is a selective antagonist of CB1 receptors, with the sibutramine. METHODS: It is an experimental clinical trial, prospective, placebo controlled. Our test was performed in 38 rats, adults females with a hyper caloric diet. We collected their blood 3 times and weighted them once a week. We divided the rats in 3 groups: Rimonabant, Sibutramine and Control. Statistic analysis has been made through ANOVA test, Tukey test and t Student test. RESULTS: The Rimonabant group demonstrated a significant reduction of the weight increase in rats. The Sibutramine group showed a significant reduction on blood glycemia compared to Rimonabant group and Control group. CONCLUSIONS: Rimonabant showed to be more effective than Sibutramine by decreasing weight gain. Sibutramine has been more effective than Rimonabant and Control groups by decreasing the blood glycemia.OBJETIVO: Comparar o efeito do rimonabanto, nova droga seletivamente antagonista dos receptores CB1, com a sibutramina. MÉTODOS: Ensaio clínico experimental, prospectivo, placebo controlado. Realizado com 38 ratas, adultas, submetidas a dieta hipercalórica. Foram coletadas 3 amostras de sangue e o peso controlado semanalmente. Foram divididas em 3 grupos: Rimonabanto, Sibutramina e Controle. Analise estatística realizada com ANOVA, teste t de Student e teste de Tukey. RESULTADOS: O grupo Rimonabanto obteve redução significante do ganho de peso. O grupo Sibutramina teve redução significativa da glicemia quando comparado aos demais grupos. CONCLUSÕES: Rimonabanto foi mais efetivo que a Sibutramina na redução do ganho de peso. A Sibutramina foi mais efetiva na redução da glicemia do que os grupos controle e Rimonabanto.

  7. Antidepressant behavioral effects of duloxetine and amitriptyline in the rat forced swimming test Efeitos antidepressivos da duloxetina e da amitriptilina no teste do nado forçado em ratos

    Directory of Open Access Journals (Sweden)

    Honório Sampaio Menezes

    2008-10-01

    Full Text Available PURPOSE: To compare the effects of the antidepressant drugs duloxetine and amitriptyline on depressive behaviors in rats. METHODS: Fifteen male Wistar rats were given systemic injections of duloxetine, amitriptyline or saline prior to a Forced Swimming Test (FST. Immobility and number of stops were measured. Data were analyzed by one-way ANOVA and Kruskall-Wallis. RESULTS: Rats given injections of duloxetine displayed fewer stops than the amitriptyline and control group (pOBJETIVO: Comparar o efeito antidepressivo da droga cloridrato de duloxetina com a amitriptilina. MÉTODOS: O teste do nado forçado, teste comportamental que avalia a atividade antidepressiva em ratos, foi utilizado em 15 ratos Wistar, machos adultos, divididos em três grupos iguais: duloxetina, amitriptilina e controle. Os dados foram analisados pelo teste One-way ANOVA e Kruskall-Wallis. RESULTADOS: Houve diferença significativa entre o número de paradas (p <0,05 entre os grupos duloxetina e amitriptilina e o grupo controle. Grupo amitriptilina e controle não apresentaram diferença (p=0,8. CONCLUSÃO: A duloxetina reduziu o comportamento depressivo sendo mais efetiva do que a amitriptilina.

  8. Efeitos da desnutrição precoce e reabilitação nutricional em ratos Effects of early malnutrition and nutritional rehabilitation in rats

    Directory of Open Access Journals (Sweden)

    Magda Lahorgue Nunes

    2002-02-01

    Full Text Available Objetivos: verificar os efeitos da desnutrição precoce e da reabilitação nutricional em ratos em desenvolvimento, em relação ao limiar para crises convulsivas, peso corporal e peso cerebral.Métodos: foram utilizadas ratas prenhas do tipo Wistar e suas ninhadas. Parte da ninhada foi submetida ao paradigma da desnutrição, e os animais restantes utilizados como controle. Em P15, animais desnutridos e do grupo controle foram submetidos a estado de mal convulsivo e, após recuperação das crises, iniciavam em período de reabilitação nutricional. Em P30, os ratos foram novamente submetidos a crise convulsiva induzida por flurothyl para determinação do limiar convulsivante. Após as crises, os ratos eram sacrificados, tendo o cérebro removido e pesado. A ninhada era pesada diariamente de P2 a P30. Resultados: observamos, a partir de P5, diferença estatisticamente significativa entre o peso corporal dos animais do grupo desnutrido em comparação ao grupo nutrido. O processo de reabilitação nutricional iniciado em P16 foi associado a ascendente ganho ponderal, entretanto a diferença de peso permaneceu até P30. O peso cerebral foi discretamente superior em animais nutridos (média 1,47 g ± 0,17 e do sexo masculino (média 1,47 g ± 0,16, entretanto a diferença não foi estatisticamente significativa em relação aos desnutridos (média 1,42 g ± 0,17 ou ao sexo feminino (1,38 g ± 0,12. O limiar (tempo em segundos para o início da primeira crise clônica e da primeira crise tônico-clônica avaliado em P15 e P30 não apresentou diferença significativa entre os grupos. Conclusões: nossos resultados sugerem que a desnutrição precoce não aumenta a susceptibilidade a crises convulsivas em ratos, no período de desenvolvimento, submetidos ao modelo do flurothyl. A reabilitação nutricional iniciada precocemente teria efeito protetor na susceptibilidade a crises convulsivas dos animais previamente desnutridos.Objective: to verify

  9. Ablation of burned skin with ultra-short pulses laser to promote healing: evaluation by optical coherence tomography, histology, {mu}ATR-FTIR and Nonlinear Microscopy; Ablacao de pele queimada com laser de pulsos ultra-curtos para promocao da cicatrizacao: avaliacao por tomografia por coerencia optica, histologia, {mu}ATR-FTIR e microscopia nao-linear

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Moises Oliveira dos

    2012-07-01

    Burns cause changes in the anatomical structure of the skin associated with trauma. The severity of the burn injury is divided into first, second and third-degree burns. The third-degree burns have been a major focus of research in search of more conservative treatments and faster results in repair for a functional and cosmetically acceptable. The conventional treatment is the use of topical natural or synthetic skin graft. An alternative therapy is the laser ablation process for burned tissue necrosis removal due to the no mechanical contact, fast application and access to difficult areas. The purpose of this study is to evaluate the feasibility of using high intensity femtosecond lasers as an adjunct treatment of burned patients. For this study, 65 Wistar rats were divided into groups of five animals: healthy skin, burned skin, two types of treatment (surgical debridement or femtosecond laser ablation) and four different times in the healing process monitoring. Three regions of the back of the animals were exposed to steam source causing third-degree burn. On the third day after the burn, one of the regions was ablated with high intensity ultrashort laser pulses ({lambda} = 785 nm, 90 fs, 2 kHz and 10 {mu}J/ pulse), the other received surgical debridement, and the last was considered the burn control. The regions were analyzed by optical coherence tomography (OCT), histology, attenuated total reflectance infrared spectroscopy using Fourier transform ({mu}-ATR-FTIR), two-photon excitation fluorescence microscopy (TPEFM) and second harmonic generation technique (SHG) on days 3, 5, 7 and 14 pos-treatments. The results showed that with the laser irradiation conditions used it was possible to remove debris from third degree burn. The techniques used to characterize the tissue allowed to verify that all treatments promoted wound healing. On the fourteenth day, the regeneration curve showed that the attenuation coefficient of laser ablated tissue converges to the values

  10. Habituation of exploratory activity in aged rats: effects of pyritinol Habituação da atividade exploratória em ratos idosos: efeitos do piritinol

    Directory of Open Access Journals (Sweden)

    Roberto DeLucia

    2005-03-01

    Full Text Available Acetylcholine (Ach is a neurotransmitter considered to play a critical role in processes underlying behavior, learning, and memory. Pyritinol (pyrithioxine, Encephabolâ, PRT is a nootropic drug that increases cholinergic transmission in the central nervous system. Behavioral habituation following multiple exposures to an open field is an experimental paradigm frequently used to assess the cognitive actions of drugs. In this paradigm the decrease of exploratory activity as a function of repeated exposure to the same environment is taken as an index memory. In the present study, effects PRT administrated to aged rats in powder form added to the diet (200 mg /kg / day, p.o., for 14 days were evaluated in the habituation to an open field. A total of 18 aged rats were randomly assigned to one of two groups control (vehicle-treated rats, n = 8 and experimental group (PRT-treated rats, n = 10 and were used in the following procedure. In the exploratory task, the post-training treatment with PRT significantly decreased the number of total area crossings and rears when compared to the control group, indicating habituation. PRT treatment enhanced retention in the total number of area crossings and rears when compared with control animals. Taken together, the data of present study suggest that muscarinic cholinergic systems could be involved in the effect of PRT in habituation of exploratory activity to an open field in aged rats.A acetilcolina é um neurotransmissor que desempenha considerável papel crítico nos processos subjacentes ao comportamento, aprendizagem e memória. Piritinol (piritioxina, Encefabolâ, PRT é um fármaco nootrópico que aumenta a transmissão colinérgica no sistema nervoso central. Habituação comportamental seguida de múltipla exposição no campo aberto é um paradigma experimental freqüentemente usado na avaliação das ações cognitivas de fármacos. Neste paradigma, a diminuição da atividade exploratória em fun

  11. Effects of L-arginine oral supplements in pregnant spontaneously hypertensive rats Efeitos da oferta oral de L-arginina em ratas prenhas espontaneamente hipertensas

    Directory of Open Access Journals (Sweden)

    José Ricardo Sousa Ayres de Moura

    2006-08-01

    Full Text Available PURPOSE: To evaluate the effects of L-arginine oral supplementation in spontaneously hypertensive pregnant rats (SHR. METHODS: Thirty SHR and ten Wistar-EPM-1 virgin female rats were used in the study. Before randomization, females were caged with males of the same strain (3:1. Pregnancy was confirmed by sperm-positive vaginal smear (Day 0. Wistar-EPM-1 rats served as counterpart control (C-1. SHR rats were randomized in 4 groups (n=10: Group Control 2, non-treated rats; Group L-Arginine treated with L-arginine 2%; Group Alpha-methyldopa treated with Alpha-methyldopa 33mg/Kg; Group L-Arginine+Alpha-methyldopa treated with L-arginine 2%+Alpha-methyldopa 33mg/Kg. L-arginine 2% solution was offered ad libitum in drinking water and Alpha-methyldopa was administered by gavage twice a day during the length of pregnancy (20 days. Blood pressure was measured by tailcuff plethysmography on days 0 and 20. Body weight was measured on days 0, 10 and 20. Results were expressed as mean ± SD (Standard Deviation. One-Way ANOVA/Tukey (or Kruskal-Wallis/Dunn, as appropriate was used for group comparisons. Statistical significance was accepted as pOBJETIVO: Avaliar os efeitos da oferta oral de L-arginina em ratas prenhas espontaneamente hipertensivas (SHR. MÉTODOS: 30 SHR e 10 Wistar-EPM-1 ratas virgens foram utilizadas no estudo. Antes da distribuição, as fêmeas foram acasaladas com machos da mesma linhagem (3:1; a prenhez foi confirmada pela presença de espermatozóides no esfregaço vaginal. As ratas Wistar-EPM-1 foram utilizadas como controles. As ratas SHR foram aleatoriamente distribuídas em 4 grupos (n=10: Grupo Controle-2, não-tratado; Grupo L-Arginina, tratado com L-arginina; Grupo Alfa-metildopa, tratado com alfa-metildopa; Grupo L-Arginina+Alfa-metildopa, tratado com arginina+Alfa-metildopa. L-arginina (2% foi oferecida ad libitum na água de beber e a Alfa-metildopa (33 mg/Kg foi administrada por gavagem, duas vezes ao dia, durante toda a

  12. Shrimp diet and skin healing strength in rats Dieta com camarão e resistência cicatricial da pele, em ratos

    Directory of Open Access Journals (Sweden)

    Elizabeth Lage Borges

    2007-06-01

    Full Text Available OBJECTIVE: Surgical scar tensile strength may be influenced by several factors such as drugs, hormones and diet. The purpose of the present study was to determine the influence of a shrimp-enriched diet on the tensile strength of rat scars. METHODS: Forty male Wistar rats were submitted to a 4 cm dorsal skin incision and the wounds were sutured with 5-0 nylon interrupted suture. The animals were divided into two groups: Group 1 (control received a regular diet, and Group 2 (experimental received a shrimp-enriched diet. The two diets contained the same amounts of proteins, lipids and carbohydrates. The rats in each group were divided into two subgroups according to the time of assessment of the scar tensile strength: subgroup A, studied on the 5th postoperative day, and subgroup B, studied on the 21st postoperative day. RESULTS: The tensile strength of the scar on the 5th postoperative day was lower in the animals that received the shrimp-enriched-diet (303.0, standard error of mean= 34.1 than in the control group (460.1, SEM = 56.7 (pOBJETIVO: A resistência cicatricial da pele pode ser influenciada por diversos fatores como medicamentos, hormônios e dieta. Este trabalho foi delineado para determinar a influência da dieta com camarão na resistência cicatricial na pele. MÉTODOS: Quarenta ratos machos Wistar foram submetidos a incisão (4cm e suturas interrompidas da pele dorsal, com fio de nylon 5-0, e foram divididos em dois grupos: o Grupo 1 (controle recebeu uma dieta convencional e Grupo 2 (experimental, recebeu dieta com adição de com camarão. As duas dietas continham quantidades semelhantes de proteína, lipídeos, e carboidratos. Os ratos de cada grupo foram divididos em dois subgrupos de acordo com os distintos períodos pós-operatórios de avaliação da resistência tecidual: subgrupo A, estudado no 5° dia pós-operatório, e subgrupo B, estudado no 21° dia pós-operatório. RESULTADOS: A resistência cicatricial da pele no 5

  13. Effects on growth after hypertension portal induced in young rats Efeitos da hipertensão portal sobre o crescimento de ratos jovens

    Directory of Open Access Journals (Sweden)

    Luiz Eduardo Correia Miranda

    2004-03-01

    Full Text Available BACKGROUND: Physical growth retardation in children with hypertension portal was observed regardless of schistosomiasis. It has been suggested that the shunt of portal blood through portosystemic collateral vessels would result in metabolic consequences that would lead to the physical growth deficit observed. AIM: Study the effects of hypertension portal in the growth of young rats. METHODS: The growth of 20 young rats, divided in the groups hypertension portal, n = 10, 103 3.7 g and sham operation n = 10, 102.6 ± 3.4 g was evaluated throughout 5 weeks and the following parameters were under observation: quality of diet offered, diet ingestion, weight increase and urinary creatinine within 24 hours. At the end of the experiment, blood was taken for biochemical tests, prothrombin time and hematocrit and hypertension portal was measured. RESULTS/CONCLUSIONS: Rats with hypertension portal induced at early stages of their lives present growth delay in the first week after surgery recovering their growth rhythm in the next weeks, catching up with the sham animals. Differences related to urinary creatinine excretion, biochemical tests and hematocrit were not observed. Such results are evidence against the hypothesis that the hypertension portal induced in early stages of rats lives would cause delay in their growth.RACIONAL: Atraso no crescimento foi observado em crianças com hipertensão portal independentemente da presença de esquistossomose. Sugeriu-se que o desvio de sangue pelas colaterais portossistêmicas justificaria os achados clínicos encontrados. OBJETIVO: Estudar os efeitos da hipertensão portal no crescimento de ratos jovens. MÉTODOS: O crescimento de 20 ratos divididos nos grupos hipertensão portal n = 10, 103 ± 3,7 g e grupo-controle, n = 10, 102,6 ± 3,4 g foi avaliado durante 5 semanas. Foram considerados a qualidade da dieta oferecida, a ingestão da dieta, o ritmo de crescimento ganho de peso, a excreção de creatinina urin

  14. Estudo da isquemia e reperfusão em retalhos cutâneos de ratos The study of the ischemia and reperfusion in skin flaps of rats

    Directory of Open Access Journals (Sweden)

    Frederico Alonso Sabino de Freitas

    2002-01-01

    Full Text Available INTRODUÇÃO: Múltiplos fatores têm sido implicados na patogênese da lesão de isquemia/reperfusão da pele, incluindo as espécies reativas de oxigênio. OBJETIVO: Estudar a lesão de isquemia/reperfusão em retalhos cutâneos de ratos avaliando os níveis teciduais do malonildialdeído (MDA e xantina oxidase (XO. MÉTODOS: Foram utilizados 8 ratos Wistar, com peso entre 300 - 400g, sendo confeccionados 2 retalhos epigástricos por animal (controle e experimento, um deles submetido à 16h de isquemia (RI seguida de 45 min de reperfusão (RR e o outro controle (RC. Foram colhidas 3 biópsias de pele dos retalhos (RC, RI, RR e encaminhadas para dosagem de MDA e XO. RESULTADOS: A análise bioquímica mostrou aumento significativo dos níveis teciduais de MDA e XO após a reperfusão em relação aos retalhos controles. CONCLUSÃO: Retalhos epigástricos de ratos submetidos à 16h de isquemia e 45min de reperfusão apresentam elevação dos níveis teciduais de MDA e XO, caracterizando a lipoperoxidação da membrana celular.INTRODUCTION: Multiple factors have been implicated in the pathogenesis of reperfusion injury in the skin, including the reactive oxygen species. OBJECTIVE: The aim was to evaluate the effect of reperfusion injury in the rat skin flap evaluated by tissue assay for malonyldialdehyde (MDA and xanthine oxidase (XO. METHODS: 8 Wistar rats were used, between 300-400g weight and two identical epigastric flaps were raised in each animal (control and experiment, the vasculature of one flap was left intact and in the second flap the arterial pedicle was clamped for 16 hours and reperfused for 45 minutes. Skin samples were obtained from each flap after these periods of time and submitted to MDA and XO analysis. RESULTS: Reperfused flaps had significantly increased MDA and XO values compared to the control flaps biopsies. CONCLUSION: The lipid peroxidation levels were higher in the rat epigastric skin flaps subjected to 16 hours of

  15. Hypothalamic interaction with the mesolimbic DA system in the control of the maternal and sexual behaviors in rats.

    Science.gov (United States)

    Stolzenberg, Danielle S; Numan, Michael

    2011-01-01

    The medial preoptic area (MPOA) of the hypothalamus regulates maternal behavior, male sexual behavior, and female sexual behavior. Functional neuroanatomical evidence indicates that the appetitive aspects of maternal behavior are regulated through MPOA interactions with the mesolimbic dopamine (DA) system; a major focus of this review is to explore whether or not the MPOA participates in the appetitive aspects of sexual behavior via its interaction with the mesolimbic DA system. A second focus of this review is to examine the extent to which estradiol interactions with DA within this circuit regulate all three reproductive behaviors. One mechanism through which estradiol activates male sexual behavior is through the potentiation of DA activity in the MPOA. In the hypothalamus, estradiol has also been found to act in concert with DA, through the activation of similar intracellular signaling pathways, in order to stimulate female sexual behavior. Finally, recent evidence suggests that some effects of estradiol are mediated by direct action of estradiol on the mesolimbic DA system. Copyright © 2010 Elsevier Ltd. All rights reserved.

  16. Antidepressant behavioral effects of duloxetine and fluoxetine in the rat forced swimming test Efeitos antidepressivos da duloxetina e da fluoxetina no teste do nado forçado em ratos

    Directory of Open Access Journals (Sweden)

    Leandro Ciulla

    2007-10-01

    Full Text Available PURPOSE: To compare the effects of the antidepressant drugs duloxetine and fluoxetine on depressive behaviors in rodents. METHODS: Eighteen male Wistar rats were given systemic injections of duloxetine, fluoxetine, or saline prior to a Forced Swimming Test (FST. Immobility and number of stops were measured. RESULTS: Rats given injections of fluoxetine displayed significantly less immobility (p = 0.02 and fewer stops than the control group (p = 0.003. Duloxetine significanlty reduced the number of stops (p = 0.003, but did not effect immobility (p = 0.48. CONCLUSION: Duloxetine and fluoxetine reduced depressive behaviors in the Forced FST. However, our findings suggest that fluoxetine is more effective than duloxetine.OBJETIVO: Comparar o efeito antidepressivo da droga cloridrato de duloxetina com a fluoxetina. MÉTODOS: O teste do nado forçado, teste comportamental que avalia a atividade antidepressiva em ratos, foi utilizado em 18 ratos Wistar, machos adultos, divididos em três grupos iguais: duloxetina, fluoxetina e controle. RESULTADOS: Os dados do teste do nado forçado foram analisados pelo teste One-way ANOVA, Mann Whitney e Kruskall-Wallis.Houve diferença significativa (p = 0,003 entre o número de paradas dos grupos duloxetina e fluoxetina e o grupo controle. CONCLUSÃO: A duloxetina e a fluoxetina tiveram frequência de paradas similares. A fluoxetina mostrou ser mais efetiva que a duloxetina no teste do nado forçado em ratos.

  17. Effect of topical application of fibronectin in duodenal wound healing in rats Efeito da aplicação tópica da fibronectina em feridas duodenais de ratos

    Directory of Open Access Journals (Sweden)

    Teresa Neuma de Souza Brito

    2003-03-01

    Full Text Available Fibronectin (FN, a large family of plasma and extracellular matrix glycoproteins, plays an important role in wound healing. PURPOSE: To evaluate the effect of fibronectin on the healing of sutured duodenal wounds, correlating with the serum and tissue level of the substance. METHODS: An experimental study was done in 30 adult Wistar rats divided into two group. In the control group (n=15 a duodenal suture was treated with saline solution 0,9% and in the test group the duodenal wounds were treated with 1% FN. The duodenal wound healing process was studied in the 5th, 7tn and 10th postoperative days, by histological sections stained by hematoxylin-eosin, Masson trichromic and immunohistochemical reaction for FN. A digital histological grading system was used to obtain a score for each group and to observe the healing process. RESULTS: the FN was present in the several layers of the duodenum and the cellular and plasmatic FN increased with the evolution of healing. In the test group the FN enhanced the wound healing within 5, 7 and 10 days after injury, when compared with the control group. CONCLUSION: The topical use of FN in duodenal sutured wounds in rats enhances healing by stimulating the appearence of fibroblasts into the wound site and development of granulation tissue. This acceleration of the repair process may have an important application in the healing of duodenal wounds.A fibronectina (FN, um componente da grande família das glicoproteínas do plasma e da matriz extracelular, desempenha um importante papel na cicatrização das feridas. OBJETIVO: Avaliar os efeitos da fibronectina na cicatrização de lesões duodenais suturadas, e estabelecer correlação dos parâmetros de cicatrização com os níveis tissulares e séricos da substância. MÉTODOS: Foi realizado estudo experimental com 30 ratos Wistar adultos dividos em dois grupos. No grupo de controle (n=15 uma lesão duodenal suturada foi tratada com aplicação tópica de 1ml

  18. Rats

    Directory of Open Access Journals (Sweden)

    Alexey Kondrashov

    2012-01-01

    Full Text Available We aimed to perform a chemical analysis of both Alibernet red wine and an alcohol-free Alibernet red wine extract (AWE and to investigate the effects of AWE on nitric oxide and reactive oxygen species production as well as blood pressure development in normotensive Wistar Kyoto (WKY and spontaneously hypertensive rats (SHRs. Total antioxidant capacity together with total phenolic and selected mineral content was measured in wine and AWE. Young 6-week-old male WKY and SHR were treated with AWE (24,2 mg/kg/day for 3 weeks. Total NOS and SOD activities, eNOS and SOD1 protein expressions, and superoxide production were determined in the tissues. Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine. The AWE increased NOS activity in the left ventricle, aorta, and kidney of SHR, while it did not change NOS activity in WKY rats. Similarly, increased SOD activity in the plasma and left ventricle was observed in SHR only. There were no changes in eNOS and SOD1 expressions. In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR. Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.

  19. Studying the central control of food intake and obesity in rats Estudando em ratos o controle central da ingestão alimentar e a obesidade

    Directory of Open Access Journals (Sweden)

    Eliane Beraldi Ribeiro

    2009-02-01

    Full Text Available The central nervous system regulates energy intake and expenditure through a complex network of neurotransmitters and neuromodulators. It is of great interest to understand the relevance of these systems to the physiological control of energy balance and to the disturbances of obesity. The present paper discusses some of the methods to address this field used at the laboratory of Endocrine Physiology of Universidade Federal de São Paulo. Initially, different experimental models of rat obesity are presented, namely the hypothalamic induced monosodium glutamate model, the Zucker genetic model, and the dietary model. The principles of brain microdialysis are also presented, the technique applied to obtain representative samples of the extracellular fluid of brain sites involved in feeding control. The microdialysate levels of serotonin, an important anorexigenic neurotransmitter, are determined by HPLC with electrochemical detection. The immunoblot technique (Western blot is used to determine hypothalamic levels of proteins relevant to the anorexigenic effect of serotonin and to analyze the acute activation of the insulin signaling cascade in the hypothalamus. The final section addresses the potential applications of proteomics in the study of the central control of feeding.O sistema nervoso central controla a ingestão e o gasto de energia por meio de um complexo circuito de neurotransmissores e neuromoduladores. É de grande interesse entender a relevância fisiológica destes sistemas e o papel que desempenham nos distúrbios da obesidade. No presente artigo, discutem-se alguns dos métodos que têm sido utilizados no laboratório de Fisiologia Endócrina da Universidade Federal de São Paulo, em estudos neste campo. Inicialmente, são apresentados alguns modelos de obesidade experimental em ratos, como a obesidade hipotalâmica induzida por glutamato monossódico, o modelo genético Zucker e também obesidades induzidas por dieta. Comenta

  20. Thermal response of rat fibroblasts stably transfected with the human 70-kDa heat shock protein-encoding gene

    International Nuclear Information System (INIS)

    Li, G.C.; Li, Ligeng; Liu, Yunkang; Mak, J.Y.; Chen, Lili; Lee, W.M.F.

    1991-01-01

    The major heat shock protein hsp70 is synthesized by cells of a wide variety of organisms in response to heat shock or other environmental stresses and is assumed to play an important role in protecting cells from thermal stress. The authors have tested this hypothesis directly by transfecting a constitutively expressed recombinant human hsp70-encoding gene into rat fibroblasts and examining the relationship between the levels of human hsp70 expressed and thermal resistance of the stably transfected rat cells. Successful transfection and expression of the gene for human hsp70 were characterized by RNA hybridization analysis, low-dimensional gel electrophoresis, and immunoblot analysis. When individual cloned cell lines were exposed to 45C and their thermal survivals were determined by colony-formation assay, they found that the expression of human hsp70 conferred heat resistance to the rat cells. These results reinforce the hypothesis that hsp70 has a protective function against thermal stress

  1. SPECT for smokers brain perfusion evaluation; SPECT para avaliacao da perfusao cerebral em fumantes

    Energy Technology Data Exchange (ETDEWEB)

    Maliska, C.M.; Martins, E.F.; Barros, D.S.; Lopes, M.M.S.; Lourenco, C.; Goncalves, S.; Goncalves, M.B.; Miranda, M.M.B.G.; Neto, L.M.; Penque, E.; Antonucci, J.B. [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil)

    2002-07-01

    Cigarette smoking increases brain stroke risk, however,five to fifteen years after ceasing this habit, brain perfusion recovers normal pattern. We propose to compare brain perfusion patterns performing brain SPECT scans of smokers and non-smokers. Thirteen volunteers age range 20-30 years old) composed of six non-smokers and seven smokers were studied by brain scans (SPECT).We used ECD- {sup 99m} Tc radiolabelled as recommended by the manufacturer. All patients received 740 MBq of the radiopharmaceutical through intravenous injection, with their eyes opened and their ears unplugged, in a quiet room. We used a one head SPECT camera ( General Electric/ StarCam 4000 and Siemens/ E.Cam) with low-energy ultrahigh resolution collimator. Imaging begin 40 minutes after tracer injection. For reconstruction we used a Butterworth filter.The preliminary results showed brain perfusion deficit areas on five of the smokers while all non- smokers had normal brain perfusion scans. We believe that smoking increases the possibility of brain perfusion deficits. (author)

  2. Integrity assessment of pipelines - additional remarks; Avaliacao da integridade de dutos - observacoes adicionais

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Luis F.C. [PETROBRAS S.A., Salvador, BA (Brazil). Unidade de Negocios. Exploracao e Producao

    2005-07-01

    Integrity assessment of pipelines is part of a process that aims to enhance the operating safety of pipelines. During this task, questions related to the interpretation of inspection reports and the way of regarding the impact of several parameters on the pipeline integrity normally come up. In order to satisfactorily answer such questions, the integrity assessment team must be able to suitably approach different subjects such as corrosion control and monitoring, assessment of metal loss and geometric anomalies, and third party activities. This paper presents additional remarks on some of these questions based on the integrity assessment of almost fifty pipelines that has been done at PETROBRAS/E and P Bahia over the past eight years. (author)

  3. Avaliacao da gestao ambiental dos hoteis de selva na Amazonia, Brasil

    Directory of Open Access Journals (Sweden)

    Gomes de Moraes, Adriana

    2008-10-01

    Full Text Available In the challenge of desvelar of that it forms the hoteleiros enterprises they manage its ambient action without causing great impacts in one of biggest ecosystems of the world, the Amazonian forest, was tried to know in this research the forms of ambient management of the located hotels of forest in the legal Amazônia. The norteadora question of the research was to know that type of ambient management is made by the hotels of selva?Foi used as instrument of collection of data the questionnaire, that if subdividiui in four great subjects to be searched. The first one was relative questions to the planning of the place, according to to the profile of the customer, third to the room and the relative questions architecture the building subject relative questions to the energy resources and infrastructure of the public services. As result of this analysis was concluded that two of three hotels searched are practising management ambient of form less impactante, since type of construction adopted until services offered to guests, or either shows objective that its hotel is coadjuvante for customer that visits this type of place, that stops it does not import luxury, comfort and amenities, more yes contact with the fauna and flora and way of life of the natives.

  4. Quality evaluation of blood irradiation process;Avaliacao da qualidade do processo de irradiacao de hemocomponentes

    Energy Technology Data Exchange (ETDEWEB)

    Goto, R.E.; Medeiros, R.B. [Universidade Federal de Sao Paulo (DDI/UNIFESP), SP (Brazil). Dept. de Diagnostico por Imagem. Coordenadoria de Fisica e Higiene das Radiacoes

    2009-07-01

    An adverse reaction in blood transfusion is the graft versus host disease (GVHD). This disease affects immunodeficient or immunosuppressed patients where transfused T cells proliferate and initiate a host's immune system reaction. Blood gamma irradiation is the most efficient way to inhibit lymphocyte T blastic transformation and mitotic activity and it is the most effective method to combat this disorder. However, there is no fast and effective system to analyze the dose distribution in the irradiation process and guarantee that the absorbed doses are in agreement with FDA recommendations. This study aims to establish a periodic quality control of the gammacell irradiators using radiochromic films properly calibrated. The quality control is simply and effective to combat GVHD as well as is useful to detect mechanical fails at rotation set system through the dose uniformity analysis. It was possible to verify the uniformity ranged from -72.38% to 106.33% and some blood bag received doses not compatible with recommended international threshold. (author)

  5. Radiological evaluation of the cranio vertebral junction; Avaliacao radiologica da transicao cranio-vertebral

    Energy Technology Data Exchange (ETDEWEB)

    Mello, Silvia Marcal Benicio de; Haetinger, Rainer Guilherme [Med Imagem - Beneficiencia Portuguesa de Sao Paulo, SP (Brazil). Setor de Cabeca e Pescoco]. E-mail: sbm@uol.com.br; Schettini, Marianna Cunha; Lima, Sergio Santos [Med Imagem - Beneficiencia Portuguesa de Sao Paulo, SP (Brazil); Mourao, Maria Lucia; Mendonca, Renato Adam [Med Imagem - Beneficiencia Portuguesa de Sao Paulo, SP (Brazil). Setor de Neuroradiologia

    2005-04-15

    The cranio vertebral junction (CVJ) comprises the occiput, atlas, their joints and ligaments. Besides conventional x-rays other imaging methods are need for the assessment of CVJ including high resolution computerized tomography (CT) and magnetic resonance imaging (MRI) which provide specific parameters such as field of view, slice thickness and incremental movement of the patient. A dynamic study is also important to assess the stability of the cranio vertebral junction and the effect upon the bulbomedullary junction. The aim of this study is to review the techniques used in the evaluation of the CVJ, the semiological parameters, and the most frequent disorders affecting this region. We review the literature and present conventional x-ray, CT and MRI images from the didactic file of the Radiology service 'Med Imagem - Beneficiencia Portuguesa de Sao Paulo, SP, Brazil, of cases with normal anatomy and the main congenital and acquired disorders of the CVJ. In daily practice, CVJ disorders are diagnoses using CT and MRI scans of the head and cervical spine. It is essential to be familiar with these conditions in order to preform specific tests which will allow a detailed study of the CVJ, thus providing the basis for the treatment, whenever indicated. (author)

  6. Crystallinity evaluation of polyhydroxybutyrate and polycaprolactone blends; Avaliacao da cristalinidade de blendas de polihidroxibutirato e policaprolactona

    Energy Technology Data Exchange (ETDEWEB)

    Cavalcante, Maxwell P.; Rodrigues, Elton Jorge R.; Tavares, Maria Ines B., E-mail: maxdpc@gmail.com [Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ (Brazil). Instituto de Macromoleculas

    2015-07-01

    Polyhydroxybutyrate, PHB, is a polymer obtained through bacterial or synthetic pathways. It has been used in the biomedical field as a matrix for drug delivery, medical implants and as scaffold material for tissue engineering. PHB has high structural organization, which makes it highly crystalline and brittle, making biodegradation difficult, reducing its employability. In order to enhance the mechanical and biological properties of PHB, blends with other polymers, biocompatible or not, are researched and produced. In this regard, blends of PHB and polycaprolactone, PCL, another biopolymer widely used in the biomedical industry, were obtained via solution casting and were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC) and low field nuclear magnetic resonance (LF-NMR). Results have shown a dependence between PHB's crystallinity index and PCL quantity employed to obtain the blends.(author)

  7. Evaluation of occupational exposure in intraoral radiography; Avaliacao da dose ocupacional em radiografia intraoral

    Energy Technology Data Exchange (ETDEWEB)

    Miguel, Cristiano; Barros, Frieda S.; Rocha, Anna S.P.S.; Godoi, Walmor C., E-mail: miguel_cristianoch@yahoo.com.br, E-mail: saicla@utfpr.edu.br, E-mail: annarocha@yahoo.com, E-mail: walmor.godoi@gmail.com [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Tilly Junior, Joao G., E-mail: joao.tilly@derax.com.br [Universidade Federal do Parana (HC/UFPR), Curitiba, PR (Brazil). Hospital das Clinicas

    2014-07-01

    The intraoral radiography is widely performed in the dental office due to low cost and agility. The doses in intraoral radiology are considered low, however it is known that doses below the threshold for deterministic radiation has the potential to induce stochastic effects. An intraoral radiography has a risk of inducing fatal cancer or serious in order of 1:10,000,000. Besides the patient, the dentist may also be being exposed to radiation during the work with the radiographics practices. The bibliographies demonstrates the lack of information on radiation protection of dentists, however, the occupational dose reduction was observed in radiology over the past 14 years. This work aims to evaluate the effective dose of radiation to which workers can be exposed dentists in dental offices to perform intraoral radiographs. In this context, a study was be conducted between June 2013 and May 2014 with 44 professionals in Curitiba city. For each dentist was given a personal dosimeter to be used for 30 days. During this period, the number of radiographies and the length of the cable triggers of the X-ray equipment was registered and, the dosimeter´s dose was read. It was observed that the cables triggers meet regulatory standards and allow dentists to get the mean minimum distance of two meters from the radiation source in 93% of cases. Through analysis of the doses, it was concluded that occupational exposures of these workers are within the recommended threshold by regulatory 453/1998 of the Ministry of Health from Brazil. (author)

  8. Imaging assessment of osteitis pubis; Avaliacao por imagem da osteite pubica

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Alexia Abuhid; Ferreira, Ana Paula Alves; Leite, Ana Rachel Albuquerque de Moura; Guido, Daniela Araujo; Rodrigues, Rogeria Nobre [Axial Centro de Imagem, Belo Horizonte, MG (Brazil)]. E-mail: ce@cidbh.com.br; Araujo, Rodrigo Otavio Dias [Minas Tenis Clube, Belo Horizonte, MG (Brazil); Lasmar, Rodrigo Campos Pace [Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte, MG (Brazil). Curso de Fisioterapia

    2005-10-15

    Objective: to assess the physiopathological aspects of osteitis pubis and the findings seen on imaging methods, emphasizing the usefulness of Flamingo view radiographs and the diagnostic aspects of Magnetic resonance images. Material and Method: magnetic resonance images of ten patients (eight of them professional athletes) referring symptoms in the inguinal regional were retrospectively evaluated. For some of these patients Flaming view radiographs were performed. Results: in all studied patients magnetic resonance imaging was the only diagnostic method capable of showing the different phases os osteitis pubis, from acute cases with pubic bone marrow edematous changes to chronic cases with involvement of other pelvic joints. Flamingo view radiographs were performed in six patients and three of them were diagnosed with vertical pubis symphyseal instability greater than 2 mm of vertical displacement. This diagnosis was confirmed by instability tests using orthopedic manoeuvres. Conclusion: magnetic resonance imaging was the most helpful method for the diagnosis of different phases of osteitis pubis whereas conventional X-ray was inconclusive in the initial phases. Flamingo view radiographs were extremely important to show vertical instability of the pubic symphysis, which in combination with osteitis pubis can be the cause of failure of nonoperative treatment. (author)

  9. Magnetomotive colon elastography: preliminary assessment; Elastografia magnetomotriz da regiao do colon: avaliacao preliminar em phantoms

    Energy Technology Data Exchange (ETDEWEB)

    Bruno, A. Colello, E-mail: alexandrecolellobruno@usp.br [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina; Grillo, F.W.; Sampaio, D.R.T.; Carneiro, A.A.O. [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Filosofia Ciencias e Letras

    2015-08-15

    Colorectal cancer (CRC) is the third most common malignant neoplasm worldwide and early diagnosis reduces morbidity. The standard preventive exams methods are uncomfortable for the patient, invasive, and /or are ionizing. Here, we evaluate the potential of magneto-motive ultrasound (MMUS) as a new, minimally invasive CRC screening technique. We developed a hybrid transducer (comprised of an ultrasound probe and a magnetic coil system) to construct relative elastography maps in a paraffin phantom with isoechoic inclusions. The electromagnetic component of our system manipulated ferromagnetic fluid located inside of our synthetic colon, and the captured ultrasound images were used to produce relative elastography maps. The MMUS images reveal by otherwise invisible structures based on differences in stiffness. Ultrasound elastography (relative) images by MMUs technique complements usual preventive CRC exams, is minimally invasive, has relative low cost when compared with others image methods. Also is fast diagnose and more comfortable for patient which prevents withdrawal of the screening. (author)

  10. Lipoic acid increases glutathione peroxidase, Na+, K+-ATPase and acetylcholinesterase activities in rat hippocampus after pilocarpine-induced seizures? O ácido lipóico aumenta as atividades da glutationa peroxidase, da Na+, K+-ATPase e da acetilcolinesterase no hipocampo de ratos após convulsões induzidas por pilocarpina?

    Directory of Open Access Journals (Sweden)

    Geane Felix de Souza

    2010-08-01

    Full Text Available In the present study we investigated the effects of lipoic acid (LA on acetylcholinesterase (AChE, glutathione peroxidase (GPx and Na+, K+-ATPase activities in rat hippocampus during seizures. Wistar rats were treated with 0.9% saline (i.p., control group, lipoic acid (20 mg/kg, i.p., LA group, pilocarpine (400 mg/kg, i.p., P400 group, and the association of pilocarpine (400 mg/kg, i.p. plus LA (20 mg/kg, i.p., 30 min before of administration of P400 (LA plus P400 group. After the treatments all groups were observed for 1 h. In P400 group, there was a significant increase in GPx activity as well as a decrease in AChE and Na+, K+-ATPase activities after seizures. In turn, LA plus P400 abolished the appearance of seizures and reversed the decreased in AChE and Na+, K+-ATPase activities produced by seizures, when compared to the P400 seizing group. The results from the present study demonstrate that preadministration of LA abolished seizure episodes induced by pilocarpine in rat, probably by increasing AChE and Na+, K+-ATPase activities in rat hippocampus.No presente estudo nós investigamos os efeitos do ácido lipóico (AL sobre as atividades da acetilcolinesterase (AChE, da glutationa peroxidase (GPx e da Na+, K+-ATPase no hipocampo de ratos durante crises convulsivas. Ratos Wistar foram tratados com solução salina a 0,9% (i.p., grupo controle, ácido lipóico (20 mg/kg, i.p., grupo AL, pilocarpina (400 mg/kg, i.p., grupo P400, e a associação de AL (20 mg/kg, i.p. com a pilocarpina (400 mg/kg, i.p., 30 min antes da administração de pilocarpina (grupo AL + P400. Após os tratamentos todos os grupos foram observados durante 1 h. No grupo P400, houve um aumento significativo na atividade da GPx, assim como uma diminuição das atividades da AChE e Na+, K+-ATPase. Por sua vez, o pré-tratamento com AL aboliu o aparecimento de convulsões e reverteu a diminuição das atividades da AChE e da Na+, K+-ATPase causadas pelas convulsões, quando

  11. Efeitos da inalação passiva da fumaça de cigarro sobre as pregas vocais de ratos Effects of passive smoke inhalation on the vocal cords of rats

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    Josilene Luciene Duarte

    2006-04-01

    Full Text Available Poucos estudos demonstram as reações patológicas devidas à inalação de fumaça pelas vias aéreas de ratos. OBJETIVO: Estudar e analisar os possíveis efeitos histopatologicos produzidos pela inalação crônica de fumaça de cigarro nas pregas vocais de ratos. DESENHO DE ESTUDO: Experimental. MATERIAL E MÉTODOS: 36 ratos masculinos (Rattus norvergicus Wistar, de 60 dias de idade, foram mantidos em gaiolas e expostos à inalação da fumaça produzida por 10 cigarros, 3 vezes ao dia, 7 dias por semana, para períodos de 25, 50 e 75 dias, e os controles respectivos. Os animais foram sacrificados e as suas laringes dissecadas e submetidas a análise histológica com coloração de Hematoxilina e Eosina e analisados através de microscopia simples. RESULTADOS: Os ratos expostos ao cigarro exibiram menor (p Few studies have demonstrated the pathologic reactions yielded by smoke inhalation on the airway in rats. AIM: The aim of this study was to analyze the possible histopathological effects produced by chronic cigarette smoke inhalation on the vocal folds of rats. STUDY DESIGN: Experimental. MATERIAL AND METHOD: 36 male rats (Rattus norvergicus Wistar strain, aged 60 days, were kept in cages and exposed to inhalation of the smoke produced by 10 cigarettes lit 3 times a day, 7 days a week, for periods of 25, 50 and 75 days, and their respective controls. Thereafter the animals were killed and their larynxes were dissected and submitted to histological processing for achievement of histological sections, which were stained with Hematoxylin and Eosin and analyzed by light microscopy. RESULTS: The rats exposed to smoke displayed smaller (p< 0,05 body mass than the control group. There was hyperplasia and squamous metaplasia in the free edge of the vocal fold and squamous hyperplasia on the middle portion of the vocal fold in all 3 study periods. Moreover, the 50-day group revealed keratinizing metaplasia in this area. Morphological alterations in

  12. Effects of uterine cervix constriction on Wistar rats Efeitos da constrição do cérvix uterino em ratos Wistar

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    Cassio Eduardo Raposo-Amaral

    2010-12-01

    Full Text Available PURPOSE: To verify if uterine cerclage can induce craniosynostosis or any cranial deformity in new born Wistar rats. METHODS: One pregnant female Wistar rat underwent laparotomy on day 18 of gestation and the uterus cervix was closed with a 3-0 nylon suture to avoid delivery, that occurs normally on the 21 day. The suture was released after 48 hours beyond the normal gestation period. The female rat delivered 11 pups. Six surviving rats from the delivery (group A - constrained group. Two rats were born from another mother and in the same age were used as control group (group B - 2 nonconstrained controls were allowed to grow. They were sacrificed 1.2 years after their birth all the eight animals. Linear measurement, routine histology and computed tomography of the skull were performed at the time of their death to evaluate the cranial asymmetries by mesurements of the anatomical landmarks of the craniofacial skeleton of the rats on the two groups and compared then. RESULTS: We did not observe statistically significant differences in any of the compared measurements (p>0.05 obtained through the morphologic and radiologic methods. Histologic examinations did not reveal any sign of premature fusion or suture imbrications. Critical decrease in longitudinal body size was noticed as the limbs too in all the animals of group A. CONCLUSION: Constriction of uterine cervix leads to fetus suffering, even death for a few animals, associated to small body size, but not to craniosynostosis.OBJETIVO: Verificar se a cerclagem intra-uterina pode induzir, ao nascimento de ratos Wistar, craniossinostose ou qualquer outra deformidade craniana. MÉTODOS: Uma rata Wistar prenhe foi submetida à laparotomia no 18º dia de gestação e o cérvix uterino foi suturado com 3-0 nylon, impedindo o parto normal que normalmente ocorre no 21º dia de gestação. A sutura foi liberada 48 horas após o período gestacional normal. A rata gestante deu à luz 11 animais. Seis

  13. Purification and partial characterization of analogous 26-kDa rat submandibular and parotid gland integral membrane phosphoproteins that may have a role in exocytosis.

    Science.gov (United States)

    Quissell, D O; Deisher, L M

    1992-04-01

    Rat submandibular and parotid gland exocytosis is primarily controlled by beta-adrenergic receptor stimulation. Although its precise role in the regulation of salivary gland exocytosis is not fully understood, protein phosphorylation, mediated by the activation of cAMP-dependent protein kinase, may be directly involved. Previous studies suggest that analogous 26-kDa integral membrane phosphoproteins may play a direct role in regulating exocytosis. Studies were here undertaken to purify and partially characterize both phosphoproteins. After endogenous phosphorylation with 32P, subcellular fraction and solubilization of the microsomal fraction in n-octyl beta-glucopyranoside, the 26-kDa integral membrane phosphoproteins were purified by high performance liquid chromatography (HPLC), followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and electroelution of the proteins. Amino acid analysis indicated a significant number of serine amino acids: N-terminal sequence data demonstrated a high level of homology; and trypsin digestion followed by reversed-phase HPLC indicated the possibility of multiple phosphorylation sites.

  14. Evaluation of the transporter-mediated herb-drug interaction potential of DA-9801, a standardized dioscorea extract for diabetic neuropathy, in human in vitro and rat in vivo.

    Science.gov (United States)

    Song, Im-Sook; Kong, Tae Yeon; Jeong, Hyeon-Uk; Kim, Eun Nam; Kwon, Soon-Sang; Kang, Hee Eun; Choi, Sang-Zin; Son, Miwon; Lee, Hye Suk

    2014-07-17

    Drug transporters play important roles in the absorption, distribution, and elimination of drugs and thereby, modulate drug efficacy and toxicity. With a growing use of poly pharmacy, concurrent administration of herbal extracts that modulate transporter activities with drugs can cause serious adverse reactions. Therefore, prediction and evaluation of drug-drug interaction potential is important in the clinic and in the drug development process. DA-9801, comprising a mixed extract of Dioscoreae rhizoma and Dioscorea nipponica Makino, is a new standardized extract currently being evaluated for diabetic peripheral neuropathy in a phase II clinical study. The inhibitory effects of DA-9801 on the transport functions of organic cation transporter (OCT)1, OCT2, organic anion transporter (OAT)1, OAT3, organic anion transporting polypeptide (OATP)1B1, OATP1B3, P-glycoprotein (P-gp), and breast cancer resistance protein (BCRP) were investigated in HEK293 or LLC-PK1 cells. The effects of DA-9801 on the pharmacokinetics of relevant substrate drugs of these transporters were also examined in vivo in rats. DA-9801 inhibited the in vitro transport activities of OCT1, OCT2, OAT3, and OATP1B1, with IC50 values of 106, 174, 48.1, and 273 μg/mL, respectively, while the other transporters were not inhibited by 300 μg/mL DA-9801. To investigate whether this inhibitory effect of DA-9801 on OCT1, OCT2, and OAT3 could change the pharmacokinetics of their substrates in vivo, we measured the pharmacokinetics of cimetidine, a substrate for OCT1, OCT2, and OAT3, and of furosemide, a substrate for OAT1 and OAT3, by co-administration of DA-9801 at a single oral dose of 1,000 mg/kg. Pre-dose of DA-9801 5 min or 2 h prior to cimetidine administration decreased the Cmax of cimetidine in rats. However, DA-9801 did not affect the elimination parameters such as half-life, clearance, or amount excreted in the urine, suggesting that it did not inhibit elimination process of cimetidine, which is

  15. Efeitos da fratura do arco zigomático no crescimento facial em ratos jovens Effect of zygomatic arch fracture on facial growth in young rats

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    Elza Maria Villanova Fernandes ROCHA

    1999-01-01

    Full Text Available Os efeitos da fratura unilateral do arco zigomático no crescimento facial foram avaliados através de mensurações cefalométricas. Fratura com desvio medial no lado direito foi realizada em ratos com um mês de idade. Os animais foram sacrificados com três meses de idade e sua mandíbula foi desarticulada. O crânio foi submetido à tomada radiográfica axial e as hemimandíbulas à norma lateral. As mensurações foram realizadas através de um sistema de computador. Foi verificada diferença significante a menor para a altura do corpo e do ramo da mandíbula. Não houve diferença significante para a profundidade da fossa infratemporal, bem como para as diversas mensurações na maxila e para o comprimento mandibular. A tendência de retorno do arco zigomático com desvio à sua posição original foi confirmada.The effect of unilateral zygomatic arch fracture on facial growth was analysed by means of cephalometric measurements. Medially displaced fracture of the right side was achieved in one month old rats. Reaching three months the animals were sacrified, and their mandibles disarticulated. The skull was submitted to axial radiographic incidence and the hemimandibles to a lateral radiographic incidence. The measurements were made through a computer system. Significant differences were verified with a decrease in body height and ramus of the mandible. There was no significant difference in infratemporal fossae depth, as well as in diverse measurements in maxilla and in mandibular lenght. The tendency of the fractured arch to return to its original position was confirmed.

  16. Effects of ligature-induced periodontitis in pregnant Wistar rats Efeito da doença periodontal induzida por ligadura na prenhez de ratas Wistar

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    Mariane Ponzio de Azevedo Galvão

    2003-03-01

    Full Text Available The aim of this study was to evaluate the influence of ligature-induced periodontal disease in pregnant rats on their newborn's health parameters. Twenty-four female adult Wistar rats were divided into two groups: the control group (G1 and the group that was submitted to dental ligatures around second upper molars (G2. After the four week period of development of periodontitis, the female animals were mated with male adult Wistar rats. There were no differences in the body weight of females between the two groups during mating and pregnancy. No differences were observed among the groups in relation to the viable newborn index. However, there were differences in newborn birth weight, explained by the diverse size of the litters. In this study, ligature-induced periodontal disease did not promote changes during pregnancy that resulted in low birth weight in newborn Wistar rats.O objetivo do presente estudo foi avaliar a influência da periodontite induzida por ligadura em ratas prenhes sobre parâmetros de saúde geral de seus filhotes. Vinte e quatro ratas Wistar de idade adulta foram divididas em dois grupos: grupo controle (G1 e grupo experimental, que recebeu ligaduras ao redor dos segundos molares superiores (G2. Após o período de indução de periodontite (quatro semanas, as ratas foram colocadas para cruzamento com ratos Wistar machos, adultos. Não houve diferença no peso corporal das fêmeas durante os períodos de cruzamento e prenhez. Também não foram observadas diferenças entre os grupos quanto à taxa de recém-nascidos viáveis. No entanto, houve diferença quanto ao peso dos recém-nascidos, sendo tal diferença explicada pela variação no tamanho das ninhadas. No presente estudo, doença periodontal induzida por ligadura não promoveu mudanças durante a prenhez que resultassem em baixo peso ao nascer dos filhotes Wistar.

  17. Interferência do intervalo de administração da droga sobre a nefrotoxicidade da gentamicina em ratos Influence of the dose regimen on the gentamicin nephrotoxicity in rats

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    Verônica Cunha Rodrigues Oliveira

    2001-06-01

    Full Text Available A insuficiência renal aguda (IRA que apresenta índice de mortalidade em torno de 50%, pode ser definida como um abrupto declínio da filtração glomerular, resultante de isquemia ou toxicidade. A nefrotoxicidade por drogas é uma das etiologias mais freqüentes (27% e sugere-se que o intervalo de administração da droga pode interferir neste efeito colateral, entretanto o melhor regime de administração ainda não está bem estabelecido. Este conhecimento proporcionaria uma atuação mais direcionada de enfermagem na prevenção desta IRA hospitalar. Os resultados obtidos nesta pesquisa, indicam que a infusão única de gentamicina determina menor nefrotoxicidade, provavelmente devido à redução da sua concentração plasmática nas 24hs, diminuindo o acúmulo intracelular deste fármaco, um dos principais mecanismos celulares deste tipo de lesão. Este regime de tratamento mostra portanto vantagens quanto ao custo, efeito nefrotóxico e segurança quanto à eficácia terapêutica.The acute renal failure (ARF, that still presents a righ mortality rate (50% can be defined as an abrupt decline of the glomerular filtration, resultant of isquemic or toxicity event. The drugs nephrotoxicity is one of the most frequent cause (27% of ARF and it is suggested that the interval of administration of the drug can interfere in this side effect, however the best administration regimen is not very well established. This study evaluated the renal function of rats that received gentamicin (100mg/kg in one dose or in two doses (2x 50mg/kg,by intraperitoneal infusion. The results obtained in this research, indicated that the single infusion of gentamicin determined smaller nephrotoxicity by the reduction of serum concentration of this drug in 24 hours, decreasing the intracelular accumulation of this gentamicin, which is one of the main cellular mechanisms of this renal injury. The single dose treatment regime, otherwise, showes advantages not only related

  18. Synthesis and evaluation of antimalarial activity of curcumin derivatives; Sintese e avaliacao da atividade antimalarica de compostos derivados da curcumina

    Energy Technology Data Exchange (ETDEWEB)

    Gomes, Patricia Ramos; Miguel, Fabio Balbino; Almeida, Mauro Vieira de; Couri, Mara Rubia Costa [Universidade Federal de Juiz de Fora (UFSJ), MG (Brazil). Instituto de Ciencias Exatas. Departamento de Quimica; Oliveira, Michael Eder de; Ferreira, Vanessa Viana; Guimaraes, Daniel Silqueira Martins; Lima, Aline Brito de; Barbosa, Camila de Souza; Oliveira, Mariana Amorim de; Almeida, Mauro Vieira de; Viana, Gustavo Henrique Ribeiro; Varotti, Fernando de Pilla, E-mail: varotti@ufsj.edu.br [Universidade Federal de Sao Joao Del Rei (UFSJ), MG (Brazil). Centro de Ciencias da Saude; others, and

    2014-05-15

    ne of the main challenges in the development of new antimalarial drugs is to achieve a viable lead candidate with good pharmacokinetic properties. Curcumin has a broad range of biological activities, including antimalarial activity. Herein, we report the antimalarial activity of six curcumin derivatives (6-12) and an initial analysis of their pharmacokinetic properties. Five compounds have demonstrated potent activity against the P. falciparum in vitro (IC{sub 50} values ranging from 1.7 to 15.2 μg mL{sup -1}), with moderate or low cytotoxicity against the HeLa cell line. The substitution of the carbonyl group in 6 by a 2,4-dinitrophenylhydrazone group (to afford 11) increases the Selective Index. These preliminary results indicate curcumin derivatives as potential antimalarial compounds. (author)

  19. Evaluation of the numerical solution of polymer flooding; Avaliacao da solucao numerica da injecao de polimeros em reservatorios de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, Vinicius Ligiero; Pires, Adolfo Puime; Bedrikovetsky, Pavel G. [Universidade Estadual do Norte Fluminense (UENF), Macae, RJ (Brazil). Lab. de Engenharia e Exploracao do Petroleo (LENEP)

    2004-07-01

    Enhanced Oil Recovery (EOR) methods include injection of different fluids into reservoirs to improve oil displacement. The EOR methods may be classified into the following kinds: injection of chemical solutions, injection of solvents and thermal methods. The chemical fluids most commonly injected are polymers, surfactants, micellar solutions, etc. Displacement of oil by any of these fluids involves complex physico-chemical processes of interphase mass transfer, phase transitions and transport properties changes. These processes can be divided into two main categories: thermodynamical and hydrodynamical ones. They occur simultaneously during the displacement, and are coupled in the modern mathematical models of EOR. The model for one-dimensional displacement of oil by polymer solutions is analyzed in this paper. The Courant number is fixed, and we compare the results of different runs of a numerical simulator with the analytical solution of this problem. Each run corresponds to a different spatial discretization. (author)

  20. Efeito da tibolona sobre o endométrio de ratas castradas Effect of tibolone on endometrium of castrated rats

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    José Augusto Soares Pantaleão

    2009-03-01

    Full Text Available OBJETIVO: avaliar o efeito do uso prolongado de alta dose de tibolona na morfologia do endométrio em ratas castradas. MÉTODOS: foram utilizadas 15 ratas Wistar, fêmeas, com idade de oito semanas e peso médio de 250 g. Todas as ratas foram submetidas à ooforectomia bilateral e 30 dias depois foi coletada citologia vaginal, verificando-se o status de menopausa. As ratas foram divididas aleatoriamente em dois grupos: o tratado (n=9 recebeu via oral 1 mg tibolona/dia; o controle (n=6 recebeu apenas solução do veículo carboximetilcelulose. Após 20 semanas de tratamento, todos os animais foram sedados e sacrificados por deslocamento cervical. Os úteros foram retirados e fixados em formol 10% tamponado. Ambos os cornos uterinos foram clivados em três regiões (proximal, medial, distal e processados para inclusão em parafina. Cortes histológicos corados com hematoxilina-eosina foram submetidos à análise morfológica e morfométrica. Foram avaliados os seguintes parâmetros: espessura do epitélio superficial endometrial, espessura do estroma endometrial, área endometrial, número absoluto de glândulas endometriais e número de glândulas/área endometrial. Os dados obtidos foram comparados mediante o teste t de Student. RESULTADOS: no Grupo Tibolona os úteros se apresentaram bem desenvolvidos e houve aumento significativo (pPURPOSE: to evaluate the effect of long-term use of a high dose of tibolone on the morphology of the endometrium of castrated female rats. METHODS: fifteen female Wistar rats, aged eight weeks and weighting about 250 g were used. All the female rats were submitted to bilateral oophorectomy and 30 days afterwards, vaginal cytology was collected, to verify the menopause status. The female rats were randomly divided in two groups. The Treatment Group (n=9 received 1 mg of tibolone/day orally; the Control Group (n=6 received a solution of carboxymethylcellulose vehicle. After 20 weeks of treatment, all the animals were

  1. Influence of arterial hypertension on colonic healing in rats Influência da hipertensão arterial na cicatrização colônica em ratos

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    Gilberto Luiz Ortolan

    2012-08-01

    Full Text Available PURPOSE: Evaluation of colonic healing in spontaneously hypertensive rats. METHODS: Fifty male, young and inbred rats were used. Twenty-five Wistar Kyoto rats (WKY as control and twenty-five spontaneously hypertensive rats (SHR as an experimental group. Colotomy and bowel suture at 2.5 cm from the peritoneal reflection were performed. All animals were allocated randomly into sub-groups for review at the third, seventh and fourteenth days after surgery. We evaluated the concentration of angiotensin II, the burst pressure, epithelialization, the organization of the tunics of the bowel wall, inflammatory response and collagen deposition. RESULTS: The burst pressure, epithelialization, organization of the tunics and collagen deposition was not significant between groups. The inflammatory reaction was more intense in the control group on the third postoperative day (p=0.023 as the experimental group on the remaining time. CONCLUSION: Systemic arterial hypertension in rats did not influence significantly the healing process of colonic anastomoses.OBJETIVO: Avaliar a cicatrização colônica em ratos espontaneamente hipertensos. MÉTODOS: Cinquenta ratos machos, jovens e isogênicos. Vinte e cinco ratos da linhagem Wistar Kyoto (WKY como controle e vinte e cinco ratos espontaneamente hipertensos (SHR como grupo experimento. Realizou-se colotomia e colorrafia a 2,5 cm da reflexão peritoneal. Alocaram-se os animais aleatoriamente em sub-grupos para avaliação no terceiro, sétimo e décimo quarto dias de pós-operatório. Foram avaliados a concentração de angiotensina II, a resistência da anastomose à insuflação, a epitelização, a organização das túnicas da parede intestinal, a reação inflamatória e a deposição de colágeno. RESULTADOS: A avaliação da resistência da anastomose, epitelização, organização das túnicas e deposição de colágeno não foi significativa entre os grupos. A reação inflamatória foi mais intensa no

  2. Mecanismos da intoxicação do fígado de rato causada pelo gossipol Mechanisms of the intoxication of rat liver caused by gossypol

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    Wanderson Luís de Carvalho

    2013-03-01

    Full Text Available O fígado desempenha uma função central no metabolismo devido à sua interposição entre o trato digestivo e a circulação geral do organismo. Ele é também o principal órgão envolvido na biotransformação de substâncias exógenas (xenobióticos, com capacidade de converter compostos hidrofóbicos em hidrossolúveis, mais facilmente eliminados pelo organismo. O gossipol é uma substância fenólica tóxica presente na semente de algodão (Gossypium sp. Com o objetivo de estudar os mecanismos envolvidos na hepatotoxicidade do gossipol avaliou-se os seus efeitos no sistema antioxidante do fígado de ratos no que diz respeito ao estresse oxidativo e aspectos histopatológicos. Foram utilizados ratos machos da linhagem Wistar, separados em dois grupos, sendo que um recebeu óleo de canola (veículo, grupo Controle e o outro recebeu gossipol na dosagem de 40 mg/kg de peso vivo do animal por 15 dias (grupo Tratado. O tratamento com gossipol promoveu alterações na atividade sérica das enzimas marcadoras de dano hepático e um significativo estresse oxidativo caracterizado pela diminuição nos níveis da glutationa reduzida (GSH e consequente aumento da glutationa oxidada (GSSG, incluindo, ainda, danos à membrana plasmática e de organelas demonstrados pela peroxidação lipídica. O resultado da avaliação histopatológica demonstrou degeneração dos hepatócitos.The liver plays a central role in metabolism due to its interposition between the digestive tract and the general circulation of the organism. It is also the main organ involved in biotransformation of exogenous substances (xenobiotics, with ability to convert hydrophobic compounds in water-soluble, more easily eliminated by the body. Gossypol is a toxic phenolic substance present in cotton seed (Gossypium sp.. Aiming to study the mechanisms involved in the hepatotoxicity of gossypol we evaluate its effects on the antioxidant system of rat liver performing an experiment that

  3. Visualization of acute liver damage induced by cycloheximide in rats using PET with [(18F]FEDAC, a radiotracer for translocator protein (18 kDa.

    Directory of Open Access Journals (Sweden)

    Akiko Hatori

    Full Text Available Liver damage induced by drug toxicity is an important concern for both medical doctors and patients. The aim of this study was to noninvasively visualize acute liver damage using positron emission tomography (PET with N-benzyl-N-methyl-2-[7,8-dihydro-7-(2-[(18F]fluoroethyl-8-oxo-2-phenyl-9H-purin-9-yl]acetamide ([(18F]FEDAC, a radiotracer specific for translocator protein (18 kDa, TSPO as a biomarker for inflammation, and to determine cellular sources enriching TSPO expression in the liver. A mild acute liver damage model was prepared by a single intraperitoneal injection of cycloheximide (CHX into rats. Treatment with CHX induced apoptosis and necrotic changes in hepatocytes with slight neutrophil infiltration. The uptake of radioactivity in the rat livers was measured with PET after injection of [(18F]FEDAC. The uptake of [(18F]FEDAC increased in livers damaged from treatment with CHX compared to the controls. Presence of TSPO was examined in the liver tissue using quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical assays. mRNA expression of TSPO was elevated in the damaged livers compared to the controls, and the level was correlated with the [(18F]FEDAC uptake and severity of damage. TSPO expression in the damaged liver sections was mainly found in macrophages (Kupffer cells and neutrophils, but not in hepatocytes. The elevation of TSPO mRNA expression was derived from the increase of the number of macrophages with TSPO and neutrophils with TSPO in damaged livers. From this study we considered that PET imaging with [(18F]FEDAC represented the mild liver damage through the enhanced TSPO signal in inflammatory cells. We conclude that this method may be a useful tool for diagnosis in early stage of acute liver damage.

  4. Effect of nitrofen in the final stages of development of the diaphragm muscle in rats Efeito do nitrofen na fase final do desenvolvimento da musculatura do diafragma em ratos

    Directory of Open Access Journals (Sweden)

    Frances Lilian Lanhellas Gonçalves

    2013-01-01

    Full Text Available PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH. METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC, composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether diluted in olive oil on gestational day (GD 9.5, whose fetuses developed CDH (N+ or not (N-, and Olive Oil Placebo (OO, composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days. We obtained body weight (BW and photographed the diaphragm area (DA, hernia area (HA and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ for myosin.} RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p OBJETIVO: Avaliar a expressão da miosina na muscularização do diafragma na hérnia diafragmática congênita (CDH experimental. MÉTODOS: Fetos de ratas foram divididos em quatro grupos: Controle Externo (EC, composto de ratas não manipuladas; Nitrofen, composto de ratas que receberam 100 mg de nitrofen (2,4-dicloro-4'nitrodifenil éter diluído no azeite no dia de gestação (GD 9.5, cujos fetos desenvolveram CDH (N+ ou não (N- e Placebo óleo de oliva (OO, composto de ratas que ingeriram apenas óleo no mesmo GD. Os fetos foram coletados com 18,5, 19,5, 20,5 e 21,5 GD (termo = 22 dias. Foi obtido o peso corporal (BW e tiradas fotografias da área do diafragma (DA, da hérnia (HA e calculada a relação HA/DA no grupo N+. Amostras de diafragmas foram processadas histologicamente para coloração com H/E e imunohistoquímica. RESULTADOS: Os fetos dos grupos N- e N+ tiveram BW e DA diminuídos em relação aos grupos EC e OO (p<0.001. Só houve diferença na HA entre os GD 18.5 e 21

  5. The Pharmacokinetics and Biodistribution of a 64 kDa PolyPEG Star Polymer After Subcutaneous and Pulmonary Administration to Rats.

    Science.gov (United States)

    Khor, Song Yang; Hu, Jinming; McLeod, Victoria M; Quinn, John F; Porter, Christopher J H; Whittaker, Michael R; Kaminskas, Lisa M; Davis, Thomas P

    2016-01-01

    PolyPEG star polymers have potential utility as cost-effective polymeric drug delivery vehicles, and as such, it is important to develop an understanding of their biopharmaceutical behavior. Moreover, although a number of studies have evaluated the utility of PolyPEG stars in vitro, investigation of these novel materials in vivo has been limited. Herein, we evaluated the pharmacokinetics of a 64 kDa tritiated PEG-based star polymer after subcutaneous and pulmonary administration in rats. After subcutaneous administration, the star polymer showed near complete bioavailability (∼80%) and a similar organ biodistribution profile to the polymer after intravenous administration. After intratracheal instillation to the lungs, the star polymer showed limited bioavailability (∼3%), and most of the administered radiolabel was recovered in lung tissue and feces after 6 d. The data reported here suggest that star polymers display similar pharmaceutical behavior to PEGylated dendrimers after subcutaneous and inhaled delivery and may therefore be used as similar, but more cost-effective drug delivery vehicles. Copyright © 2016. Published by Elsevier Inc.

  6. Anatomopathological study of cardiomyopathy induced by doxorubicin in rats Estudo anatomopatológico da miocardiopatia induzida pela doxorrubicina em ratos

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    José Carlos Dorsa Vieira Pontes

    2010-04-01

    Full Text Available PURPOSE: The development of an experimental model of myocardiopathy induced by Doxorubicin in rats. METHODS: 16 wistar male rats were randomized in two groups: Group I (placebo and Group II (Doxorubicin - 5mg/kg. After six months, the animals were subjected to cardiotomy and their hearts were weighted and submitted to transversal cuts, from which fragments for a macro and micro study were obtained. These fragments were studied considering their external and internal diameters and the thickness of the left ventricle (LV. The histological pieces were analyzed for the presence of fibrosis, cytoplasmic vacuolization, necrosis and size of nucleus variation. Data obtained was submitted to statistical analysis with Student's t test. RESULTS: The hearts of the animals in Group II increased 41% in relation to their weight; 33% in the internal diameter and 14% in the external diameter of the LV cavity; and 24% in the thickness of the wall. Fibrosis of the myocardial tissue was observed in 75% of the animals of Group II; all the animals presented miocyte cytoplasmatic vacuolization; myocardial necrosis was present in 75% of the animals; and 87/% presented variation in the size of myocite nuclei. The presence of polymorphonuclear cells was also observed. CONCLUSION: Doxorubicin was effective in the promotion of macro and microscopic alterations in the cardiac tissue of rats, possibly constituting a model for the experimental study of myocardiopathy.OBJETIVO: Desenvolver um modelo experimental de miocardiopatia induzida por doxorrubicina em ratos. MÉTODOS: 16 ratos Wistar machos foram randomizados em 2 grupos: Grupo I (placebo e Grupo II (doxorrubicina 5mg/kg. Após 6 meses, os animais foram submetidos a cardiotomia e seus corações foram pesados e submetidos a cortes transversais. Estes fragmentos foram estudados considerando seus diâmetros externos e internos e a espessura do ventrículo esquerdo. As peças histológicas foram analisadas quanto

  7. Estudo experimental da ação da metilprednisolona utilizada antes do traumatismo raquimedular em ratos Wistar Experimental study on the action of methylprednisolone on Wistar rats before spinal cord injury

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    Raphael Martus Marcon

    2010-01-01

    Full Text Available OBJETIVO: Avaliar os efeitos da metilprednisolona empregada previamente ao traumatismo medular, tanto em relação aos possíveis efeitos benéficos quanto às possíveis complicações associadas. MATERIAIS E MÉTODOS: Foram utilizados 32 ratos Wistar, divididos em 4 grupos. Dois grupos receberam as drogas A (placebo e B (metilprednisolona imediatamente após a lesão. Outros 2 grupos receberam as mesmas drogas 4 horas antes da lesão. Todos foram avaliados por um período de 28 dias quanto à função locomotora e complicações associadas. RESULTADOS: Os 4 grupos foram comparados quanto ao peso e idade. Não foi encontrada diferença estatisticamente significante entre os grupos de estudos quanto às médias de peso e de idade. Na comparação entre os 4 grupos quanto às intercorrências foi encontrada diferença estatisticamente significante nos óbitos (p = 0,047, onde o grupo Droga B T0 apresentou proporção de óbitos (0% significantemente menor do que a encontrada no grupo Droga B T-4 (55,6%. Não houve diferença estatística entre estres grupos quanto aos índides motores e quanto às complicações (p > 0,05 em todas as comparações. CONCLUSÕES: os animais tratados com metilprednisolona quatro horas antes do trauma apresentaram um número de óbitos significativamente maior quando comparados aos ratos tratados com a mesma droga após o trauma.OBJECTIVE: To evaluate the effects of methylprednisolone used prior to spinal injury, both in relation to possible beneficial effects and to possible associated complications. MATERIALS AND METHODS: The study subjects were 32 Wister rats, divided into 4 groups. Two groups received drugs A (placebo and B (methylprednisolone immediately after the injury. Another 2 groups received the same drugs 4 hours before the injury. They were all evaluated over a period of 28 days to verify locomotor function and associated complications. RESULTS: The 4 groups were compared in terms of weight and age. No

  8. Efeitos das isoflavonas em altas doses sobre o útero da rata Effects of high-dose isoflavones on rat uterus

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    Adriana Aparecida Ferraz Carbonel

    2011-10-01

    Full Text Available OBJETIVO: Avaliar o efeito de altas doses de isoflavonas no útero de ratas adultas castradas. MÉTODOS: Ratas virgens ovariectomizadas (n = 40 foram tratadas por 30 dias consecutivos com veículo (GCtrl ou genisteína nas concentrações 42 (GES42, 125 (GES125 e 250 (GES250 µg/g de peso corporal ao dia. O extrato de soja e o veículo (propilenoglicol foram administrados por gavagem. Ao final do experimento, foi realizada dosagem sérica de 17 β-estradiol e progesterona, avaliou-se o peso dos animais e dos úteros e foi feito exame colpocitológico. Fragmentos do terço médio dos cornos uterinos foram fixados em formol a 10% e processados para inclusão em parafina para estudo histológico. Cortes de 5 µm de espessura foram corados pelo HE e destinados a estudo em microscopia de luz. Analisou-se a histomorfologia do endométrio, área endometrial, número e área ocupada pelas glândulas, assim como a concentração de eosinófilos presentes na lâmina própria. Os dados numéricos obtidos foram submetidos à análise de variância complementada pelo teste de Tukey-Kramer (p GES125 do que nos outros grupos (GES250 > GES125 > GES42 = GCtrl; p OBJECTIVE: To evaluate the effects of high-dose isoflavones on the uterus of castrated adult rats. METHODS: Adult, ovariectomized virgin rats (n = 40 were treated by gavage during 30 consecutive days with vehicle (propylene glycol, group GCtrl or different doses of genistein: 42 (group GES42, 125 (GES125, or 250 (GES250 µg/g body weight per day. Animals were killed, weighed, vaginal and uterine samples were taken for cytologic evaluation, and serum levels of 17 β-estradiol and progesterone were determined. The middle third of the uterine horns was dissected, fixed in 10% formaldehyde and processed for paraffin inclusion; 5-µm thick sections were obtained and stained with HE for further histological study under light microscopy. The endometrial morphology and area, number and area of glands, and number

  9. Effects of aging on abdominal wall healing in rats Efeitos do envelhecimento na cicatrização da parede abdominal, em ratos

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    Maria de Lourdes Pessole Biondo-Simões

    2005-04-01

    Full Text Available PURPOSE: The aim of this study was to assess abdominal wall healing in old and young adult rats. METHODS: On average, young animals were 110 days old and old animals were 762 days old. A 4.0 cm median laparotomy was performed under anesthesia, followed by laparorrhaphy on two synthesis planes, i.e. peritoneum-muscle-aponeurosis and skin, using continuous 5.0 nylon sutures. The animals were evaluated on the 3rd, 7th, 14th and 21st postoperative days. The resistance of the two planes was studied separately and a histopathologic analysis was performed on sections stained with hematoxylin-eosin and Sirius Red. Immunohistochemical analysis was also carried out using PCNA, LCA and CD34. RESULTS: The skin scars gained resistance in a similar manner at the initial time points, but those of young rats were more resistant on the 21st day (p=0.0029. Total and type III collagen content was similar in the two groups and type I collagen content was higher in young animals on the 14th day. Inflammatory cell infiltration was more marked in the skin wounds of young animals on the 3rd day (p=0.0190. Reepithelialization was similar and angiogenesis was more intense in the skin wounds of young animals on the 14th day (p=0.0062. The peritoneum-muscle-aponeurosis wounds gained similar resistance during the early phases, but were more resistant on the 14th day (p=0.0005 and on the 21st day (p=0.0023 in old rats Collagen concentration was higher in the wounds of old animals on the 3rd day (p=0.0112 and in the wounds of young animals on the 21st day (p=0.0348. The inflammatory reaction was more intense in the wounds of old animals on the 3rd day (p=0.0060 and angiogenesis was more intense on the 14th day (0.0432. CONCLUSION: Although there are some differences in the healing course between young and old animals, age, of itself, does not impair the healing of abdominal wall wounds in rats.OBJETIVO: Estudar a cicatrização da parede abdominal em ratos adultos jovens e

  10. Uso do cianoacrilato na síntese da pele de ratos trabalho de pesquisa experimental Use of cyanoacrylate as surgical adhesive in rats skin incisions: experimental study

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    Cláudio Carneiro Borba

    2000-03-01

    Full Text Available O objetivo deste estudo foi investigar o uso dos adesivos etil-cianoacrilato e butil-cianoacrilato com o náilon monofilamentar, comparativamente , na síntese da pele de ratos. Trinta e dois ratos foram distribuídos em três grupos: A, B e C. Estes grupos foram divididos em dois subgrupos, para estudo no sétimo e no décimo-quarto dia pós-operatório. Foram efetuadas no abdome do rato, duas incisões longitudinais e paralelas distando cada uma delas um centímetro da linha mediana, sendo uma do lado direito e a outra do lado esquerdo. Em todos os grupos a síntese da incisão do lado direito, foi realizada com pontos separados de náilon 5-zeros, sendo este considerado o grupo controle (C. Nos animais do grupo A, a síntese do lado esquerdo foi realizada com etil-cianoacrilato (Super Bonder e nos animais do grupo B, a síntese foi realizada com butil-cianoacrilato (Histoacryl. No sétimo e no décimo-quarto dia pós-operatório, o aspecto macroscópico da cicatriz não mostrou diferença significante.No aspecto microscópico.os grupos B e C foram melhores do que o A. Os resultados demonstraram que a síntese com o adesivo sintético é eficaz, mais rápida, indolor e com bons resultados estéticos.Cyanoacrylate derivates have been used as surgical adhesives, like ethyl-cyanoacrylate(Super-Bonder and buthyl-cyanoacrylate(Histoacryl for many years.In this study, the histotoxicity and binding ability of Super-Bonder and Histoacryl were compared.32 rats were operated, and distributed in 3 groups :A, B and C. Each group was subdivived in 2 subgroups , to be studied after 7 and 14 days postoperatory .2 longitudinals incisions with 3 centimetre in lenght, were done in each animal. The left incision was sutured with Nylon 5.0 (group C as controls and the right incision was sutured with ethil-cyanoacrylate (group A and buthil-cyanoacrylaty (group B. Our results allowed us to clearly study tissue acute inflammation, the presence of dehiscense and

  11. Efeito da somatotropina sobre a cicatrização de feridas cutâneas, em ratos Effect of somatotropin on skin wound healing in rats

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    Luciana de Oliveira Marques dos Santos

    2002-08-01

    Full Text Available A somatotropina, além do efeito anabolizante, age no processo de cicatrização acelerando a formação local de tecido de granulação, síntese e deposição de colágeno, quando administrada por via subcutânea. Objetivo: Avaliar o efeito da somatotropina sobre a cicatrização de feridas cutâneas, em ratos. Métodos: Utilizaram-se 53 ratos Wistar, machos, com idade média de 145,19 dias e peso inicial médio de 287,72 g, divididos em dois grupos: controle (n=26 que recebeu 1,2 ml/dia de água destilada pela via subcutânea e experimento (n=27 que recebeu 0,2 UI/kg/dia de somatotropina humana pela mesma via. Fez-se uma ferida cutânea no dorso do animal, que diariamente era medida, procedendo-se ao final do experimento o cálculo de contração da ferida. Os tempos de aferição foram 3, 7 e 14 dias, quando de 9 animais de cada grupo, coletou-se sangue para dosagem bioquímica de proteínas plasmáticas e ressecou-se a ferida para estudo histológico. À microscopia avaliou-se: epitelização, reação inflamatória local, tecido de granulação, neovascularização e fibrose. Resultados: O cálculo de contração da ferida, nos 3 tempos de aferição, não demonstrou diferenças entre os grupos controle e experimento. Na dosagem bioquímica encontrou-se diminuição das proteínas totais (p=0,007 e aumento da relação albumina/globulina (p=0,03 no 14º dia no grupo controle, enquanto o grupo experimento manteve-as constantes. Na avaliação histológica observou-se significante aumento da fibrose no 7º dia no grupo experimento (pSomatotropin, in addition to having an anabolizing effect, also acts on the healing process by accelerating the local formation of granulation tissue and collagen synthesis and deposition when administered subcutaneously (SC. Objective: To evaluate the effect of somatotropin on skin wound healing in rats. Methods: Fifty-three male Wistar rats aged on average 145.19 days and weighing 287.27 g at the beginning of

  12. Ação crônica do napsilato de propoxifeno na prenhez da rata Chronic effects of propoxyphene napsylate on pregnant rats

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    Eliane T. R. Mendes

    1998-03-01

    Full Text Available O objetivo deste trabalho é avaliar os efeitos do napsilato de propoxifeno sobre a prenhez da rata albina. Para tanto utilizamos 50 ratas prenhes divididas ao acaso em cinco grupos iguais. Todas receberam diariamente, por gavagem, o volume de 1 ml, desde os dias 0 (zero até o 20 de prenhez, com as seguintes características: grupo I - somente água destilada (controle; grupo II - solução aquosa de acácia 2% (veículo; grupos III, IV e V - respectivamente, 5, 15 e 45 mg/kg de peso de napsilato de propoxifeno dissolvido em solução de acácia a 2%. Os pesos maternos foram anotados nos dias 0 (zero, 7, 14 e 20 de prenhez; no 20º dia as matrizes foram sacrificadas. Nossos resultados mostraram que os animais tratados com 45 mg/kg do fármaco apresentaram redução dos pesos individuais dos fetos como também dos pesos das ninhadas e das placentas. Quanto às outras variáveis apreciadas: número de reabsorções, de implantações e de placentas não houve diferença significante entre os grupos tratados em relação ao grupo controle.The purpose of this study is to evaluate the effects of propoxyphene napsylate on the pregnancy of the rat. We used fifty pregnant rats divided into five groups. All the animals received daily 1 ml of the solution by gavage from day 0 to the 20th day of pregnancy. Group I - only distilled water (control; group II - aqueous solution of acacia 2% (vehicle; groups III, IV and V - respectively, 5, 15 and 45 mg/kg of weight of propoxyphene napsylate diluted in 2% acacia solution. The animals were weighed on days 0, 7, 14 and 20 of pregnancy. All animals were sacrificed on the 20th day of pregnancy. Our results showed that the animals treated with 45 mg/kg of propoxyphene napsylate presented reduction of the individual weights of the fetuses, as well as of the weights of the newborns and placentas. The difference betewwn number of resorptions, implantations and placentas of the treated groups was shown to be non

  13. Avaliação funcional e histológica da oxigenoterapia hiperbárica em ratos com lesão medular Functional and histologic evaluation of hyperbaric oxygen therapy in rats with spinal cord injury

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    Paulo Eduardo de Carvalho Galvão

    2011-01-01

    Full Text Available OBJETIVOS: Avaliar a eficácia da aplicação da oxigenoterapia hiperbárica em ratos Wistar, com lesão medular contusa produzida por equipamento computadorizado para impacto por queda de peso, NYU Impactor. MÉTODOS: Avaliaram-se 17 ratos machos com peso variando de 265 a 426 g; realizaram-se impactos com peso de 10 g de uma altura pré-determinada de 12,5 mm ao nível da décima vértebra torácica, após realização de laminectomia prévia. Os ratos foram divididos aleatoriamente em grupo controle e grupo oxigênio hiperbárico. Este último, submetido à tratamento com oxigenoterapia em câmara hiperbárica, durante uma hora diária por um período de 30 dias. A avaliação da recuperação locomotora foi realizada no 2º, 9º, 16º, 23º e 30º dia pós-operatório, avaliados através de escala funcional e o sítio de lesão submetido à exame anatomopatológico. RESULTADOS: Demonstrou-se melhora da recuperação locomotora nos ratos tratados com oxigênio hiperbárico nas fases iniciais de avaliação mas no final da avaliação não havia diferença estatisticamente significante entre ambos grupos. O exame anatomopatológico comprovou as alterações estruturais da medula espinal nos dois grupos. CONCLUSÃO: A lesão medular leve provocada nos ratos evoluiu de maneira diferente no grupo da oxigenoterapia hiperbárica comparativamente ao grupo controle, na fase inicial.OBJECTIVE: To evaluate the effectiveness of the application of hyperbaric oxygen therapy in Wistar rats with spinal cord contusion produced using computerized equipment to create impact by a falling weight, NYU Impactor. METHODS: We evaluated 17 male rats with weights ranging from 265 to 426 g; impacts were performed with a weight of 10 g from a pre-determined height of 12.5 mm, at the tenth thoracic vertebra, after completion of prior laminectomy. The rats were randomly divided into a control group and a group treated with hyperbaric oxygen. The latter, was treated with

  14. Effects of bromopride on abdominal wall healing with induced peritoneal sepsis after segmental colectomy and colonic anastomosis in rats Efeitos da bromoprida na cicatrização da parede abdominal com sepse peritoneal induzida e submetidos à ressecção segmentar e anastomose do cólon esquerdo em ratos

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    Marcos Vinícius Melo de Oliveira

    2011-12-01

    Full Text Available PURPOSE: Evaluate the effects of bromopride on abdominal wall healing of rats with induced peritoneal sepsis after segmental colectomy and colonic anastomosis. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of bromopride (bromopride group - B or saline solution (control group - C. Each group was divided into subgroups of 10 animals each to be killed on the third (GB3 and GC3 or seventh postoperative day (GB7 and GC7. It was analyzed the following characteristics: breaking strength of the abdominal wall's wound; surgical and histopathological features of the abdominal wall; and clinical features of the rats. RESULTS: There was no difference between the groups in relation to the weight of the rats and the breaking strength of the abdominal wall's wound. The GB7 group presented less edema and less quantity of fibrin during histopathological evaluation compared to the GC7 group. CONCLUSION: Bromopride did not have harmful effects on the healing of abdominal wall in rats.OBJETIVO: Avaliar o efeito da bromoprida, na cicatrização da ferida operatória da parede abdominal de ratos com sepse peritoneal experimentalmente induzida e submetidos a ressecção segmentar e anastomose de cólon esquerdo. MÉTODOS: 40 ratos distribuídos em dois grupos contendo 20 animais, para administração de bromoprida (grupo bromoprida- B ou solução de NaCl 0,9% (grupo controle - C. Cada grupo foi dividido em subgrupos contendo 10 animais, para eutanásia no terceiro (GB3 e GC3 ou sétimo dia (GB7 e GE7 de pós-operatório. Os ratos foram submetidos à secção do cólon esquerdo e anastomose término-terminal. No dia da eutanásia foram avaliadas as características cirúrgicas da cavidade abdominal e clínicas dos ratos. Foram coletados segmentos da parede para a avaliação histopatológica e de resistência tênsil da ferida operatória. RESULTADOS: N

  15. Efeitos da terapia estro-raloxifeno sobre o endométrio de ratas Effects of combined estrogen and raloxifene therapy on rat endometrium

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    Alexandre Vieira Santos Moraes

    2006-02-01

    Full Text Available OBJETIVO: avaliar os efeitos dos estrogênios conjugados eqüinos (Ece e do raloxifeno (Ral, isoladamente ou associados, sobre o endométrio de ratas adultas. MÉTODOS: foram utilizadas 56 ratas adultas, ooforectomizadas, divididas aleatoriamente em sete grupos: GCont (controle; GEce (Ece 50 µg/kg; GEce/25 (Ece 25 µg/kg; GRal/0,75 (Ral 0,75 µg/kg; GRal/0,4 (Ral 0,4 mg/kg; GEce-Ral (50/0,75 - (Ece 50 µg/kg + Ral 0,75 mg/kg e GEce-Ral (25/0,4 - (Ece 25 µg/kg + Ral 0,4 mg/kg. As drogas foram administradas por gavagem durante 21 dias consecutivos. Ao final da administração todos os animais foram anestesiados e fragmentos dos úteros removidos, fixados em formol a 10% e processados para inclusão em parafina. Os cortes obtidos foram corados por HE e submetidos à avaliação histomorfométrica. Foram avaliados os seguintes parâmetros: espessura do epitélio superficial, número de glândulas endometriais/mm² e número de vasos sangüíneos presentes no miométrio/mm². Os dados obtidos foram submetidos a análise estatística de ANOVA seguida pelo teste de Tukey-Kramer. RESULTADOS: nos grupos controle (Gcont e tratados isoladamente com raloxifeno (GRal/0,75 e GRal/0,4 o endométrio mostrou-se atrófico. Já nos grupos tratados com Ece isoladamente foi observado endométrio muito desenvolvido, sendo este efeito mais acentuado no grupo que recebeu 50 µg/kg, no qual encontramos aumento da espessura do epitélio superficial e da lâmina própria e no número de glândulas endometriais e de vasos sanguíneos. Notamos também proliferação endometrial nos grupos que receberam a associação de Ece e Ral (GEce-Ral - 50/0,75 e (GEce-Ral - 25/0,4. CONCLUSÃO: o raloxifeno parece bloquear parcialmente a ação do estrogênio no endométrio de ratas adultas castradas.PURPOSE: to evaluate the effects of conjugated equine estrogens (CEE and raloxifene (Ral, alone or combined, on the rat endometrium. METHODS: fifty-six adult rats were ovariectomized and

  16. Evaluation of the thermoluminescent detector answers of CaSO{sub 4}:Dy, LiF:Mg,Ti and micro LiF:Mg,Ti in photon clinical beams dosimetry using water simulator; Avaliacao da resposta de detectores termoluminescentes de CaSO4:Dy, LiF:Mg,Ti e microLiF:Mg,Ti na dosimetria de feixes clinicos de fotons utilizando simulador de agua

    Energy Technology Data Exchange (ETDEWEB)

    Matsushima, Luciana C.; Veneziani, Glauco R.; Campos, Leticia L., E-mail: lmatsushima@usp.b, E-mail: veneziani@ipen.b, E-mail: lcrodri@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (GMR/IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Gerencia de Metrologia das Radiacoes; Sakuraba, Roberto K.; Cruz, Jose C. da, E-mail: rsakuraba@einstein.b, E-mail: jccruz@einstein.b [Sociedade Beneficente Israelita Brasileira, Sao Paulo, SP (Brazil). Hospital Albert Einstein (HAE)

    2011-10-26

    This paper perform the comparative study of thermoluminescent answer of calcium sulfate dosemeter doped with dysprosium (DaSO{sub 4}:Dy) produced by the IPEN, Sao Paulo, with answer of lithium fluoride dosemeters doped with magnesium and titanium (LiF:Mg, Ti) in the dosimetry of clinical beams of photons (6 and 15 MV) by using water simulator object. Dose-answer curves were obtained for gamma radiation of cobalt-60 in the air and in conditions of electronic equilibrium (plate of PMMA), and clinical photons of CLINAC model 2100C accelerators of the two evaluated hospitals: Hospital das Clinicas of the Faculty of Medicine of Sao Paulo university and Hospital Albert Einstein. It was also evaluated the sensitivity and reproduction of the three dosemeters

  17. SUBACUTE AND MULTIGENERATIONAL TOXICITY EVALUATION OF CASTOR OIL POLYMER INGESTION IN RATS AVALIAÇÃO DA TOXICIDADE SUBAGUDA E MULTIGERACIONAL DA INGESTÃO DE DERIVADO DO POLÍMERO DE MAMONA EM RATOS

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    Gilberto Chierice

    2009-04-01

    Full Text Available Castor oil polymer is a biomaterial that can be used to fill bone defects. Biomaterials should present some characteristics such as: biocompatibility, inert, absence of toxin radical and vapour discharge and permit the organism to recognize them as a part of their structure. The aim of this study was to investigate changes in water ingestion, feed intake, weight gain and toxic effects in rats (Rattus norvegicus after ingestion of 0, 10, 25 and 50% of castor oil polymer (COP, Ricinus communis. In groups that received 10 and 25% of COP the weight gain was smaller than the control group. Feed intake in control group was greater than in groups 10 and 50% COP. The intake of castor oil polymer reduced feed intake and water ingest probably because of the palatability of the product. In the second part of this study (multigenerational there were no toxic effects in the offspring, as external birth defects or alterations incompatible with life, in spite of the fact that lower weight were observed in males and females of the second generation.

    KEY WORDS: Biomaterials, bone defects, polymer, Ricinus communis. Os biomateriais são polímeros artificiais desenvolvidos para substituir tecidos orgânicos danificados e preencherem falhas ósseas. Eles devem apresentar características como a biocompatibilidade, serem inertes, não liberarem vapores e radicais tóxicos e permitirem ao organismo reconhecê-los como parte de sua estrutura. Procurou-se determinar a ingestão de água, o consumo de ração, ganho de peso e possível efeito tóxico decorrentes da ingestão de três doses (10%, 25% e 50% do derivado do polímero de mamona (PM, Ricinus communis, adicionado à água de bebida, em ratos (Rattus norvegicus. Nos grupos que receberam 10% e 25% do PM, o ganho de peso foi menor (P?0,05, quando comparado ao grupo-controle. O consumo de ração nos grupos que receberam 10% e 50% de PM foi menor em relação ao grupo-controle. A ingestão do pol

  18. The use of amniotic membrane in the repair of duodenal wounds in Wistar rats Uso da membrana amniótica no reparo de feridas duodenais em ratos Wistar

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    Luciano Rodrigues Schimidt

    2010-02-01

    Full Text Available Purpose: In the search of a new material to repair duodenal wounds, a trial was conducted to assess the behavior of human amniotic membrane in the repair of the duodenal wall in rats. METHODS: Fifty Wistar rats weighing between 250 and 350g, male, were submitted to duodenotomy and randomly distributed into two groups. Group A (n=8 had no treatment and was used as the control group. In Group B (n=42 the duodenal wound was treated with a patch of human amniotic membrane. RESULTS: All animals in Group A died. In Group B no changes were observed with regards to death or the formation of duodenal fistula. All animals presented peritoneal adherences in the region on the duodenal wall repair and intestinal obstruction was observed in two animals. Healing of the duodenal wall in the region of the patch took place progressively as the post-operatory period increased, with regeneration of the mucosa and of the smooth muscle layer. CONCLUSION: From the clinical standpoint, the amniotic membrane proved to be a biological tissue which served as a temporary seal and allowed the wound to heal by second-intention, with re-establishment of the duodenal wall structure.OBJETIVO: Na busca de um novo material para o reparo dos ferimentos duodenais, foi efetuado um estudo para avaliar o comportamento da membrana amniótica humana no reparo da parede duodenal em ratos. MÉTODOS: Foram utilizados 50 ratos Wistar, com peso entre 250 e 350g, machos, distribuídos, aleatoriamente, em dois grupos. Grupo A (n=8, submetido à duodenotomia sem tratamento, utilizados como controle. Grupo B (n=42, submetido a um remendo de membrana amniótica humana para tratamento de ferimento duodenal provocado. RESULTADOS: Todos os animais do grupo A foram a óbito. No grupo B não foram observadas alterações quanto a óbito ou formação de fístula duodenal. Observaram-se em todos os animais aderências peritoneais à região do reparo da parede do duodeno e obstrução intestinal em

  19. Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(aanthracene Indução da carcinogênese mamária experimental em ratas com 7,12-dimetilbenz(aantraceno

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    Alfredo Carlos S. D. Barros

    2004-01-01

    Full Text Available PURPOSE: To test an experimental model of chemical mammary carcinogenesis induction in rats. METHODS: Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(aanthracene (DMBA intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy. RESULTS: Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%. After 13 weeks, all of them (100% developed breast carcinomas that were confirmed by histopathological analysis. CONCLUSION: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.OBJETIVO: Testar um modelo experimental de indução química de carcinogênese mamária em ratas. MATERIAL E MÉTODOS: Com 47 dias de vida, 20 ratas Sprague-Dawley, jovens e virgens, receberam por gavagem intragástrica 20 mg de 7,12-dimetilbenz(aantraceno (DMBA. Oito e 13 semanas depois da injeção de droga as mamas das ratas foram examinadas. Ao final os animais foram sacrificados e fragmentos dos tumores foram estudados ao microscópio. RESULTADO: Oito semanas depois da injeção de DMBA 16 ratas apresentavam tumor nas mamas (80%. Com 13 semanas todas desenvolveram carcinomas de mama (100%, que foram confirmados por análise histopatológica. CONCLUSÃO: Este modelo experimental de indução química de carcinogênese mamária é factível e pode ser empregado em futuras pesquisas para avaliar o papel de substâncias biomoduladoras da tumorigênese.

  20. Uptake of oleate by isolated rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut

    International Nuclear Information System (INIS)

    Schwieterman, W.; Sorrentino, D.; Potter, B.J.; Rand, J.; Kiang, C.L.; Stump, D.; Berk, P.D.

    1988-01-01

    A portion of the hepatocellular uptake of nonesterified long-chain fatty acids is mediated by a specific 40-kDa plasma membrane fatty acid binding protein, which has also been isolated from the gut. To investigate whether a similar transport process exists in other tissues with high transmembrane fatty acid fluxes, initial rates (V/sub O/) of [ 3 H]-oleate uptake into isolated rat adipocytes were studied as a function of the concentration of unbound [ 3 H]oleate in the medium. V/sub O/ reached a maximum as the concentration of unbound oleate was increased and was significantly inhibited both by phloretin and by prior incubation of the cells with Pronase. A rabbit antibody to the rat liver plasma membrane fatty acid binding protein inhibited adipocyte fatty acid uptake by up to 63% in dose-dependent fashion. Inhibition was noncompetitive; at an immunoglobulin concentration of 250 μg/ml V/sub max/ was reduced from 2480 /plus minus/ 160 to 1870 /plus minus/ 80 pmol/min per 5 /times/ 10 4 adipocytes, with no change in K/sub m/. A basic kDa adipocyte plasma membrane fatty acid binding protein, isolated from crude adipocyte plasma membrane fractions, reacted strongly in both agar gel diffusion and electrophoretic blots with the antibody raised against the corresponding hepatic plasma membrane protein. These data indicate that the uptake of oleate by rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut

  1. Is subdiaphragmatic aortic cross-clamping a suitable model for spinal cord ischemia/reperfusion injury study in rats? O pinçamento sub-diagragmático da aorta é um modelo adequado para o estudo da lesão medular de isquemia/reperfusão em ratos?

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    Sonia Elizabeth Lopez Carrillo

    2006-08-01

    Full Text Available PURPOSE: To evaluate the efficacy of subdiaphragmatic aortic cross-clamping in an experimental model of ischemia/reperfusion injury of the spinal cord in albino rats. METHODS: Thirty-six male Wistar rats were randomized in two groups (n=18: G-1 (Sham and G-2 (Ischemia/Reperfusion, I/R. G-2 rats were submitted to 30 min subdiafragmatic aortic cross-clamping. G-1 rats served as controls and were submitted to surgical trauma (laparotomy without ischemia. Samples (spinal cord and arterial blood were collected at the end of ischemic period and 10 (T-10 and 20 (T-20 min later in G-2 rats. Sham rats (G-1 samples were collected at the same time-points. Blood and tissue metabolites concentrations of pyruvate, lactate, glucose and medullary adenosine triphosphate (ATP were assayed. RESULTS: Blood and tissue concentrations of pyruvate and glucose as well as lactate and medullary ATP were not different when comparing G1 to G2. Lactacemia was significantly elevated in G-2 compared with G-1 rats during reperfusion (T-10. CONCLUSION: Subdiaphragmatic aortic cord cross-clamping is not a suitable rat model for spinal cord ischemia/reperfusion injury study as it does not ensure changes in in vivo tissue metabolites concentrations similar to those found in tissues subjected to ischemia/reperfusion.OBJETIVO: Avaliar a eficácia do pinçamento da aorta subdiafragmática no modelo experimental de isquemia/reperfusão da medula espinhal em ratos. MÉTODOS: Trinta e seis ratos Wistar, machos, foram aleatoriamente distribuídos em 2 grupos (n=18 e submetidos ao pinçamento subdiafragmático da aorta, durante 30 minutos (Grupo-2 -Isquemia/Reperfusão. Os ratos do Grupo-1 (G-1 - Sham foram utilizados como controles e submetidos a laparotomia sem pinçamento arterial. As amostras (medula e sangue arterial foram coletadas ao término do período de isquemia (T-0 e 10 (T-10 e 20 (T-20 minutos mais tarde e nos mesmos intervalos, no grupo G-1. As concentrações teciduais e

  2. Papel da luminosidade do biotério no comportamento do rato no labirinto em cruz elevado Role of vivarium illumination in rat behavior in the elevated plus-maze

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    Raquel Martinez

    2005-08-01

    Full Text Available Há pouca informação sobre o efeito dos ambientes onde os ratos são mantidos antes de testes. Para investigar o efeito da iluminação do biotério, ratos Wistar machos foram submetidos a um período de 96 h de iluminação contínua, escuridão contínua ou um ciclo claro escuro de 12:12 h e testados no labirinto em cruz elevado em uma sala iluminada (150 lux ou escura (0 lux. Os resultados mostram que nem a iluminação contínua nem a escuridão contínua do biotério afetam o comportamento dos ratos, quando comparados aos sujeitos mantidos no ciclo claro-escuro de 12 h. A condição de luminosidade durante o teste, no entanto, foi importante: independentemente da condição de iluminação do biotério, os animais testados no escuro exploraram mais os braços abertos do labirinto, um resultado já relatado na literatura e interpretado como diminuição da ansiedade nesse aparato.There is little information about the environments where rats are kept before being tested. In order to investigate the role of vivarium illumination, male Wistar rats were submitted to a 96-h period of continuous illumination, continuous dark or a 12:12 h light/dark cycle and tested in the elevated plus-maze in a lit (150 lux or a dark room (0 lux. Results showed that neither vivarium illumination nor darkness for 96 h altered the rats' behavior in comparison to that of rats kept under the 12-h light/dark cycle. Luminosity during the test, however, was important: no matter what the vivarium illumination was, rats tested in the dark room explored more the open arms of the maze, an already reported result which is interpreted as decreased anxiety in this apparatus.

  3. Immunomodulatory effect of cimetidine on the proliferative responses of splenocytes from T. cruzi-infected rats Efeito imunomodulatório da cimetidina sobre a resposta blastogênica de esplenócitos de ratos infectados por T. cruzi

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    M.N. Sato

    1991-06-01

    Full Text Available The immunomodulatory effect of cimetidine (CIM, a histamine type-2 receptor antagonist, was evaluated in respect to the blastogenic response to Con A of Wistar Furth (WF rats infected by the Y strain of Trypanosoma cruzi (T. cruzi. Enhancement of blastogenesis of normal splenocytes was observed at a concentration of 10-3M. However, the splenocytes from infected animals responded to concentrations of CIM ranging from 10-8 to 10-3M. The mitogenic response to Con A of cells from infected animals was restored in the presence of CIM. The results show that CIM modulates the "in vitro" proliferative response of cells from T. cruzi-infected rats and suggest an immunoregulatory role of histamine and/or of cells that express H2 receptors in this infection.O efeito imunomodulatório da Cimetidine (CIM, um antagonista do receptor de histamina-tipo 2, foi avaliado na resposta blastogênica a Con A em células de ratos Wistar Furth (WF infectados pela cepa Y de Trypanosoma cruzi (T. cruzi. Foi observado que apenas na concentração de 10-3M de Cimetidine houve amplificação da resposta blastogênica de esplenócitos normais a Con A. Entretanto, a capacidade mitogênica de esplenócitos de animais infectados foi restaurada na presença de molaridades da droga que variaram entre 10-8 a 10-3. Os resultados demonstraram que a CIM tem o potencial de modular a resposta mitogênica de células de animais infectados pelo T. cruzi, sugerindo um papel imunoregulatório da histamina e/ou células que expressam receptores H2 nesta infecção.

  4. DOPA, norepinephrine, and dopamine in rat tissues

    DEFF Research Database (Denmark)

    Eldrup, E; Richter, Erik; Christensen, N J

    1989-01-01

    We studied the effect of unilateral sympathectomy on rat quadriceps and gastrocnemius muscle concentrations of endogenous dihydroxyphenylalanine (DOPA), dopamine (DA), and norepinephrine (NE) and assessed the relationships between these catecholamines in several rat tissues. Catecholamines were...

  5. Evaluation of the 18 kDa translocator protein (TSPO) as a target for molecular imaging and therapy of glioblastoma in an experimental rat model

    International Nuclear Information System (INIS)

    Awde, Ali Reda

    2012-01-01

    In France alone, there are 3000 new cases of glioblastoma multiform (GBM) per year and therefore GBM is the most common and aggressive form of the primary tumor in the central nervous system (CNS). The clinical prognosis for glioblastoma patients is extremely poor with a median survival period that rarely exceeds 15 months post-diagnosis. Since the study performed by Stupp and colleagues in 2005, the standard treatment for newly diagnosed glioblastoma consists of surgical removal of the tumor, followed by radiotherapy and concomitant chemotherapy with temozolomide. The 18 kDa Translocator Protein (TSPO), previously known as the peripheral benzodiazepine receptor (PBR) is a mitochondrial membrane protein known to be implicated in cholesterol transport, protein import, transport of porphyrin, cell proliferation and apoptosis through its interaction with VDAC (Voltage-Dependent Anion Channel) in the mitochondrial permeability transition pore (PTPM). Previous studies have reported overexpression of TSPO in brain tumors, suggesting that this protein may represent a molecular target for the therapy of GBM. In particular, Erucyl-phospho-homo-choline (ErPC3, erufosine), an alkyl-phosphocholine, seems to be a promising agent in the treatment of glioblastoma. Previous studies have reported its ability to induce apoptosis in otherwise highly apoptosis resistant glioma cell lines and ErPC3 induced apoptosis seems to require the presence TSPO. [ 18 F]DPA-714, a new TSPO radioligand for positron emission tomography (PET) imaging, was developed at the CEA and validated in different models of neuro-inflammation. The hypotheses underlying this thesis are: 1) that the overexpression of TSPO in GBM can be detected by PET imaging using [ 18 F]DPA-714 and 2) that the targeting of TSPO, via specific ligands or via ErPC3, can induce apoptosis in GBM. The objectives of the thesis were: 1) to evaluate the expression of TSPO in a panel of rodent and human glioma cell lines and 2) to

  6. Estudo morfométrico da terapia LED de baixa potência em tendinite de ratos Morphometric study of low power LED therapy tendonitis in rats

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    José Mário Nunes da Silva

    2011-12-01

    Full Text Available A terapia LED de baixa potência possui efeitos analgésico e antiinflamatório. O objetivo desse estudo foi analisar a ação da terapia com LED de baixa potência na reparação tendínea por meio de histologia e histomorfometria. Foram usados 25 ratos Wistar, de 220 a 250 g, divididos em três grupos experimentais avaliados no 7º e 14º dia: A, tendinite induzida sem tratamento; B, tendinite induzida tratada com LED de baixa potência, densidade de energia 4 J/cm² por 120 segundos; e C, sem indução de tendinite. A histomorfometria mostrou-se altamente significativa (p0,05. As análises histológica e histomorfométrica demonstraram qualitativo e quantitativo aumento no número de fibroblastos aos sete dias de tratamento, e das fibras colágenas, aos 14 dias, para grupo tratado com LED de baixa potência em relação ao grupo sadio (C. O mesmo não foi percebido quando relacionado ao grupo tendinite (A14.The low power LED therapy has analgesic and anti-inflammatory effects. The objective of this study was to analyze the action of therapy with low power LED on the tendon repair, using histology and histomorphometry. Were used 25 Wistar rats, with 220 to 250 g, divided into three experimental groups (7 and 14 days: A7 and A14 induced tendonitis without treatment; B7 and B14, induced tendinitis treated with LED low power density energy 4 J /cm² for 120 seconds; and C, without induction of tendinitis. Histomorphometry was highly significant (p 0.05. Histology and histomorphometry analysis demonstrated qualitative and quantitative increase in the number of fibroblasts to seven days of treatment, and collagen fibers at 14 days for the group treated with LED low power compared to the healthy group (C. But it is unclear even when related to the tendinitis group (A14.

  7. Desnutrição neonatal e microbiota normal da cavidade oral em ratos Neonatal malnutrition and normal microbiota of the oral cavity in rats

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    Solange Maria Magalhães da Silva Porto

    2007-12-01

    Full Text Available OBJETIVO: Avaliar a influência da desnutrição neonatal sobre o padrão e o crescimento de bactérias aeróbias, da microbiota normal da cavidade oral, em ratos Wistar adultos. MÉTODOS: O material da cavidade oral foi coletado através de swabs embebidos em 40µL de solução salina estéril e colocados em tubos estéreis contendo 960µL de brain heart infusion. Posteriormente, fez-se homogeneização de cada uma amostra. Então, destes 1.000µL, retirou-se 1µL e este foi semeado em placas de Petri contendo Agar-sangue e Levine para isolamento e identificação de bactérias Gram+ e Gram-, respectivamente. Essas placas foram incubadas em estufa bacteriológica a 37ºC, 48 horas, e as unidades formadoras de colônias que cresceram foram contadas e seus percentuais calculados. Para a bacterioscopia foram confeccionadas lâminas coradas pelo método de Gram. RESULTADOS: Do 5º ao 21º dia de vida os pesos corporais do grupo desnutrido (33,6g:42,8g, desvio-padrão=27,2g foram menores (pOBJECTIVE: To evaluate the influence of neonatal malnutrition on the pattern and growth of aerobic bacteria of the normal bacterial flora of the oral cavity in adults Wistar rats. METHODS: In the present study, the material of the oral cavity was collected through swabs soaked in 40µL of sterile saline solution. After the collection, each swab was placed in a sterile tube containing 960µL of brain heart infusion. Later, the samples were homogenized. Then, from the 1.000µL, 1µL was collected with a gauged loop to be sowed in Petri dishes containing Agar-blood and Agar-Levine, for the isolation and identification of the Gram-positive and Gram-negative bacteria respectively. The plates were placed into a bacteriological incubator, 37ºC, for 48 hours and the colony-forming units that grew were counted and their percentages were calculated. For bacterioscopy, slides were stained with the Gram method. RESULTS: From the 5th to the 21st day of life, body weight of

  8. Evaluation of the macroscopic growth degree of experimental endometriosis in rats Avaliação do grau de crescimento macroscópico da endometriose experimental em ratos

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    João Nogueira Neto

    2007-01-01

    Full Text Available PURPOSE: To evaluate macroscopically the growth degree of self-transplantation of endometriosis in rats. METHODS: Forty female rats, after a 7-day period for adpating and evaluating of the estrous cycle regularity, underwent tail abdominal midline laparotomy with 3-cm cuts. The average third of the left uterine horn was removed, 4mm x 4mm patches in liquid environment were made, and self-transplanted in the rat mesenterium with a single stitch, and the endometrial surface of the endometriotic implant facing the lumen of the peritoneal cavity. The rats were programmed to die after three weeks. The abdominal cavity displaying was held and self-transplants were identified and classified. RESULTS: The results achieved were: one case for degree 0 (2,5%, three cases for degree 1 (7,5%, eleven cases for degree II (27,5% and twenty-five cases for degree III (62,5%. CONCLUSION: The experimental endometriosis development, through the self-transplantation technique, showed to be most common in degrees 3 and 2 of development.OBJETIVO: Avaliar macroscopicamente o grau de crescimento de autotransplantes de endometriose em ratos. MÉTODOS: Quarenta ratos fêmeas, após período de sete dias para adaptação e avaliação da regularidade do ciclo estral, foram submetidas à laparotomia mediana abdominal caudal com incisões de três cm. Foi retirado o terço médio do corno uterino esquerdo, feito retalhos de quatro mm x quatro mm em meio líquido, sendo em seguida autotransplantado no mesentério da rata com ponto simples, tomando o cuidado de manter a superfície mucosa voltada para luz abdominal. Após o período de três semanas as mortes das ratas foram programadas. Realizou-se a exposição da cavidade abdominal com identificação e classificação dos autotransplantes. RESULTADOS: os resultados encontrados foram: grau 0 obteve um caso (2,5%, o grau I foi observado em três casos (7,5%, o grau II com onze casos (27,5% e o grau III foi visto em vinte

  9. Conhecimento da anatomia da orelha de cobaias e ratos e sua aplicação na pesquisa otológica básica Understanding the anatomy of ears from guinea pigs and rats and its use in basic otologic research

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    Agnes Afrodite Sumarelli Albuquerque

    2009-02-01

    Full Text Available O uso de mostras animais é importante na pesquisa otológica e o conhecimento da anatomia de sua orelha permite sua utilização adequada. OBJETIVO: Estudar a anatomia da orelha da cobaia e do rato por microscopia óptica de luz (MOL e microscopia eletrônica de varredura (MEV e suas vantagens anatômicas na pesquisa otológica básica. MATERIAL E MÉTODO: Os ossos temporais, as bulas timpânicas e cócleas de três cobaias e ratos albinos foram fotografados e analisados ao MOL e MEV. RESULTADOS: O rato não é tão simples de manipular como a cobaia, e freqüentemente apresenta otite média. O rato apresenta uma junção frágil da bula timpânica, duas e meia espiras na cóclea e a membrana timpânica não veda todo o conduto auditivo externo. A cobaia possui uma bula inteiriça, martelo e bigorna fundidos e três e meia espiras na cóclea. Pela MEV a cobaia e o rato possuem Membrana Tectória, Membrana de Raissner e o Órgão de Corti. As Células de Hensen estão presentes somente na cobaia. CONCLUSÃO: A cobaia foi considerada de fácil manipulação para a microdissecção, pelo tamanho e rigidez do osso temporal, e para experimentos cirúrgicos envolvendo o estribo, janela oval e a membrana timpânica. Pela MEV nota-se semelhança entre cobaia e rato, podendo ambos serem utilizados em estudos da orelha interna.The use of animal samples is important in otologic research and understanding the anatomy of their ears help make proper use of them in research projects. AIM: to study guinea pig's and rat's ears under light microscopy(LM and scanning electron microscopy(SEM and understand their anatomical advantages in basic otologic research. MATERIALS AND METHODS: The temporal bones, tympanic bullas and cochleas from three albino guinea pigs and rats were photographed and analyzed under LM and SEM. RESULTS: Rats aren't as simple to handle as guinea pigs, and often present with otitis media. Rats have a fragile junction of the tympanic bulla

  10. Indução de empiema em ratos através da inoculação pleural de bactérias Experimental empyema in rats through intrapleural injection of bacteria

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    José Carlos Fraga

    2001-12-01

    Full Text Available OBJETIVOS: avaliar a indução experimental de empiema em ratos, através da inoculação intrapleural de duas bactérias (Pasteurella multocida e Staphylococcus aureus, utilizando técnica cirúrgica simples e de fácil execução. MÉTODOS: foram utilizados 24 ratos albinos da raça Wistar, de ambos os sexos, pesando entre 250 e 300g, que, após a anestesia geral, foram submetidos à toracotomia anterior direita, afastamento da musculatura e inoculação de 0,2ml de solução, conforme descrição a seguir: grupo I (n=12, inoculação de Pasteurella multocida, 10(10 unidades formadoras de colônia/ml cultivados em caldo cérebro-coração; grupo II (n=8, inoculação de Staphylococcus aureus, 10(10 unidades formadoras de colônia/ml cultivados em caldo cérebro-coração; e grupo III (n=4, inoculação de caldo cérebro-coração estéril (controle. Os animais foram sacrificados em até 7 dias e a intensidade da reação pleural, analisada macroscopicamente conforme escala padronizada. Também foram avaliados a mortalidade, o volume de líquido na cavidade pleural e o exame bacteriológico (animais mortos e líquido pleural. RESULTADOS: no grupo I (Pasteurella multocida, sete ratos morreram nas primeiras 48 horas de experimento. Cinco ratos foram sacrificados no período programado, mas nenhum deles apresentava empiema. No grupo II (Staphylococcus aureus, somente um animal morreu nas primeiras 24 horas, os outros 7 (88% foram sacrificados e apresentavam empiema. No grupo III, considerados controles, todos os animais sobreviveram, não se observando nenhuma anormalidade torácica ao sacrifício. Analisando conjuntamente os grupos, a indução de empiema esteve associada de maneira significativa à inoculação de Staphylococcus aureus no espaço pleural (p OBJECTIVE: to evaluate empyema formation in rats through the injection of two bacteria (Pasteurella multocida and Staphylococcus aureus, using a simple, easy-to-use surgical technique

  11. Efeitos da terapia de reposição hormonal na cicatrização de anastomoses de cólon Effects of hormonal replacement therapy on colon anastomosis healing in rats

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    Maria de Lourdes Pessole Biondo-Simões

    2005-06-01

    Full Text Available OBJETIVO: Existe coincidência entre os sintomas do climatério e o aparecimento acentuado dos sinais de envelhecimento da pele. Estudos, em modelos animais, mostraram que o estrógeno é uma espécie de mediador crítico na cicatrização de feridas. Os autores apresentam um estudo da influência da terapia de reposição hormonal (TRH em anastomoses de cólon, feitas em ratas. MÉTODOS: Utilizam 3 grupos de ratas Wistar, um grupo de ooforectomizadas com TRH feita com 50mg de estrógeno e 2 mg de acetato de medroxiprogesterona, um de ooforectomizadas e sem TRH e um de ratas laparotomizadas e não ooforectomizadas. Realizaram a ooforectomia e a confirmação da condição hormonal após 28 dias. Em seguida instituíram a TRH ou de solução fisiológica, diariamente. Após 2 meses fizeram uma colotomia esquerda com anastomose término-terminal e estudaram a resistência e a densidade de colágeno com 7 e 14 dias. RESULTADOS: As anastomoses dos cólons das ratas sem TRH eram menos resistentes do que as do grupo controle tanto no 7.º dia (p=0,0488 como no 14.º dia (p=0,0115. A densidade de colágeno foi menor no 7.º dia (p=0,0210 com menor presença de colágeno I (p=0,0023 e de colágeno III (p=0,0470. No 14.º dia estas diferenças permaneceram significantes. Nas anastomoses dos cólons das ratas com TRH as diferenças, em relação ao grupo controle, não foram significantes. CONCLUSÃO: A falta dos hormônios ovarianos leva à menor resistência e atrasa a maturação de anastomoses do cólon, em ratas e estas deficiências são compensadas pela TRH.PURPOSE: The symptoms of the climacteric coincide with the marked appearance of signs of skin aging. Studies on animal models have shown that estrogen is a critical mediator in wound healing. The authors report a study of the influence of hormonal replacement therapy (HRT on colon anastomoses performed in female rats. METHODS: Three groups of Wistar rats were used: one submitted to oophorectomy

  12. Evaluation of the crude oil viscosity variation in function of the demulsifiers addition; Avaliacao da variacao da viscosidade de oleo cru em funcao da adicao de desemulsificante

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Jansen M.; Lucas, Elisabete F. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Macromoleculas]. E-mail: elucas@ima.ufrj.br; Neves, Guilherme B.M. [COMAB Especialidades Quimicas Ltda., Rio de Janeiro, RJ (Brazil)]. E-mail: tecnico@comabrio.com

    2003-07-01

    One way of improving well production is the addition of demulsifier already in the gas lift. This is due to the apparent viscosity of water-in-oil emulsions being higher than apparent viscosity of crude oil, which in turn is higher than the apparent viscosity of an water-in-oil dual phase admixture and is also higher than the apparent viscosity of an oil-in-water emulsion. However, there are some situations where, in order to obtain separate flows of oil and water phases, demulsifier should be added in specific amounts in order to promote the desired phase separation. In heavy oils water and oil phase separation may be hard to obtain, however, the right demulsifier amount may imply in a considerable decrease in petroleum viscosity even without the appearance of two phases, making the flow easier. This work has evaluated the viscosity of a heavy crude having API degree 14 and BSW 52%, as a function of the addition of different amounts of DEMTROL BR 67, manufactured by Dow Quimica/Comab, Brazil, as demulsifier. (author)

  13. Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis Efeitos da bromoprida na cicatrização de anastomoses no cólon esquerdo de ratos sob sepse abdominal induzida

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    Silvana Marques e Silva

    2012-06-01

    Full Text Available PURPOSE: To evaluate the effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis. METHODS: Forty rats were divided into two groups to receive either bromopride (experimental group- E or saline (control group- C. Each group was divided into subgroups of ten animals each to be euthanized on third (E3 and C3 or seventh day (E7 and C7 after surgery. Sepsis was induced by cecal ligation and puncture. The rats underwent segmental left colon resection and end-to-end anastomosis. Adhesion formation, tensile strength and hydroxyproline concentration were assessed. Histomorphometry of collagen and histopathological analysis were also performed. RESULTS: On postoperative third day, anastomoses in bromopride-treated animals showed lower tensile strength (p=0.02 and greater reduction in hydroxyproline concentration (p=0.04 than in control animals. There was no statistical difference in these parameters on seventh day, and the remaining parameters were similar across subgroups. Collagen content was also similar across subgroups. CONCLUSION: In the presence of abdominal sepsis, the administration of bromopride was associated with decreased tensile strength and hydroxyproline concentration in left colonic anastomoses in rats three days after surgery.OBJETIVO: Avaliar os efeitos da bromoprida sobre a cicatrização de anastomoses de cólon esquerdo de ratos na presença de sepse abdominal. MÉTODOS: Quarenta ratos distribuídos em grupos contendo 20 animais para administração de bromoprida ou salina. Cada grupo foi dividido em subgrupos contendo dez animais, para eutanásia no terceiro ou no sétimo dia de pós-operatório. A indução da sepse foi realizada pelo método de ligadura e punção do ceco. Foi realizada ressecção de um segmento do cólon esquerdo e anastomose término-terminal. À re-laparotomia, foi avaliada a quantidade total de aderências e removido um segmento colônico contendo a anastomose

  14. Gel from unripe Musa sapientum peel to repair surgical wounds in rats Gel da casca de Musa sapientum verde no reparo de lesões operatórias em ratos

    Directory of Open Access Journals (Sweden)

    Dênia Amélia Novato Castelli Von Atzingen

    2011-10-01

    Full Text Available PURPOSE: To determine the optimum concentration of a gel obtained from unripe banana (Musa sapientum peel for wound treatment in rats. METHODS: A randomized triple blind study was conducted with 40 Wistar rats, which were divided into 4 groups: CG, control group; G2%, 2% gel concentration group; G4%, 4% gel concentration group; and G10%, 10 % gel concentration group. The banana peel gel was applied daily, for 7 days, to a 4-cm² wound created on the back of each animal of all groups. After this period, the wounds were biopsied. Statistical analysis was carried out using the Kruskal-Wallis test complemented by the Student-Newman-Keuls test. RESULTS: Macroscopic examination revealed that partial epithelialization occurred in all groups. Wound contraction was also observed in all groups and ranged from 1.38 to 1.57 mm in the study groups, and from 1.03 to 1.10 mm in the control group, with significant differences (p OBJETIVO: Avaliar a concentração ideal do gel da casca de Musa sapientum verde no tratamento de feridas em ratos. MÉTODOS: Estudo randomizado, triplo cego, com 40 ratos da linhagem Wistar divididos em quatro grupos: GC controle, G2% gel a 2%, G4% gel a 4%, G10% gel a 10%. Realizou-se aplicação diária do gel nas diferentes concentrações, durante sete dias, em uma ferida de 4 cm² realizada no dorso de cada rato. Após este período, as lesões foram biopsiadas. Para analise dos dados utilizou-se o teste de Kruskal-Wallis complementado pelo teste de Student-Newman-Keuls. RESULTADOS: Os achados macroscópicos demonstraram reepitelização parcial em todos os grupos. A contração da área da ferida variou entre 1,38 a 1,57 mm nos grupos de estudo, e entre 1,03 a 1,10 mm no grupo controle. Houve diferença significante (p < 0,05 entre os grupos: GC e G10%, G2% e G4%, G2% e G10%, sendo o desvio interquartílico menor entre os grupos GC e G4%. CONCLUSÃO: O gel a 4% da casca de M. sapientum verde promoveu maior área de epiteliza

  15. Avaliação do efeito da glicosamina e condroitina na consolidação de fratura: estudo experimental em ratos Evaluating the effects of glucosamine and chondroitin in bone healing: experimental study in rats

    Directory of Open Access Journals (Sweden)

    Roberto Guarniero

    2007-07-01

    Full Text Available OBJETIVO: Avaliar o efeito da administração da condroitina e da glicosamina na consolidação de fratura em modelo animal. MÉTODOS: Este estudo experimental envolveu a utilização de 40 ratos machos adultos da raça Lewis. Os animais foram randomicamente divididos em quatro grupos de 10 animais cada, assim constituídos: grupo I, com administração de glicosamina; grupo II, com administração de condroitina; grupo III, administração da associação de glicosamina e condroitina; grupo IV, administração de água destilada (grupo controle. Realizou-se uma fratura fechada médio-diafisária da tíbia e fíbula direitas em cada animal, seguida da administração diária das drogas de acordo com o grupo, durante 30 dias. Após esse período, os animais foram sacrificados para estudo dos calos ósseos formados. Os critérios de avaliação foram a avaliação clínica da consolidação óssea, mensuração da densidade mineral do calo ósseo utilizando-se a densitometria óssea e cálculo da área do calo formado por meio de planigrafia. Os dados coletados foram avaliados com a técnica da análise de variância (ANOVA para verificar diferenças entre as médias nos quatro grupos estudados e com o teste de Tukey para comparação das médias duas a duas. Adotou-se nível de significância de 5% (alfa = 0,05. RESULTADOS: A utilização da condroitina e da glicosamina, tanto de maneira isolada quanto associadas, não resultou em aumento da área do calo ósseo ou da sua densidade mineral óssea, e não havendo melhora clínica da consolidação óssea. CONCLUSÃO: A administração de condroitina e glicosamina, neste estudo, não influenciou - quer de maneira positiva ou negativa - a consolidação de fraturas experimentais em ratos.OBJECTIVE: To evaluate the effects of administering chondroitin and glucosamine for bone healing in an animal model. METHODS: This experimental study involved the use of 50 male adult Lewis rats. The animals were

  16. Pharmacokinetics and brain distribution of tetrahydropalmatine and tetrahydroberberine after oral administration of DA-9701, a new botanical gastroprokinetic agent, in rats.

    Science.gov (United States)

    Jung, Ji Won; Kwon, Yong Sam; Jeong, Jin Seok; Son, Miwon; Kang, Hee Eun

    2015-01-01

    DA-9701, a new botanical gastroprokinetic agent, has potential for the management of delayed gastric emptying in Parkinson's disease if it has no central anti-dopaminergic activity. Therefore, we examined the pharmacokinetics of DA-9701 components having dopamine D2 receptor antagonizing activity, tetrahydropalmatine (THP) and tetrahydroberberine (THB), following various oral doses (80-328 mg/kg) of DA-9701. The distribution of THP and THB to the brain and/or other tissues was also evaluated after single or multiple oral administrations of DA-9701. Oral administration of DA-9701 yielded dose-proportional area under the plasma concentration-time curve (AUC0-8 h) and maximum plasma concentration (Cmax) values for THP and THB, indicating linear pharmacokinetics (except for THB at the lowest dose). THP and THB's large tissue-to-plasma concentration ratios indicated considerable tissue distribution. High concentrations of THP and THB in the stomach and small intestine suggest an explanation for DA-9701's potent gastroprokinetic activity. The maximum concentrations of THP and THB in brain following multiple oral DA-9701 for 7 d (150 mg/kg/d) was observed at 30 min after the last oral DA-9701 treatment: 131±67.7 ng/g for THP and 6.97±4.03 ng/g for THB. Although both THP and THB pass through the blood-brain barrier, as indicated by brain-to-plasma concentration ratios greater than unity (approximately 2-4), oral administration of DA-9701 at the effective dose in humans is not expected to lead to sufficient brain concentrations to exert central dopamine D2 receptor antagonism.

  17. Influence of omentoplasty on colonic anastomosis in animals submitted to hemorrhagic shock in rats Influência da omentoplastia na anastomose cólica de animais submetidos a choque hemorrágico em ratos

    Directory of Open Access Journals (Sweden)

    Ricardo Bolzam-Nascimento

    2009-06-01

    Full Text Available PURPOSE: To analyze influence of omentoplasty on anastomosis in descending colon of rats. Rats were submitted to the hypovolemic shock of the hemorrhagic type by the Biomechanical Test of Pressure of Rupture by Liquid Distension (BTPRLD. In addition, establish a type of acute anemia in rats that are provided to the study. METHODS: Comparative study between two groups of animals with ten rats in each one, all submitted to hemorrhagic shock for 30% volemic removal by the carotid artery. An anastomosis was performed in left colon. An anastomosis was performed in the left colon. Group 1 took place anastomosis with Polyvinyl Chloride (P.V.C film to prevent the adhesions formation on sature line. Group 2 placed the great omentum around the anastomosis. Euthanasia occurred on the fifth day, when the anastomoses were submitted to the biomechanical test of pressure of rupture by liquid distension (BTPRLD. RESULTS: High rupture pressure was gained with omentoplasty group in relation to the group in which anastomosis was protected from adhesions formation. A statistical significance was noted. CONCLUSION: Protection by great omentum has increased the anastomosis resistance of the shocked animals. Also, the proposed hemorrhagic shock type has proven to be useful for this study.OBJETIVO: Analisar a influência da omentoplastia sobre anastomose realizada em cólon descendente de ratos que foram submetidos a choque hipovolêmico do tipo hemorrágico, por meio do Teste Biomecânico de Pressão de Ruptura à Distensão por Líquido. Além disso, estabelecer modelo de anemia aguda em rato que se preste ao referido estudo. MÉTODOS: Estudo comparativo entre dois grupos de animais com 10 ratos em cada, todos submetidos a choque hemorrágico por retirada volêmica de 30% através da artéria carótida, sendo realizada anastomose em cólon esquerdo. No grupo 1 realizou-se proteção da anastomose com película de polivinilcloreto para impedir a formação de ader

  18. 64 kDa protein is a candidate for a thyrotropin-releasing hormone receptor in prolactin-producing rat pituitary tumor cells (GH4C1 cells)

    International Nuclear Information System (INIS)

    Wright, M.; Hogset, A.; Alestrom, P.; Gautvik, K.M.

    1988-01-01

    A thyrotropin-releasing hormone (TRH) binding protein of 64 kDa has been identified by covalently crosslinking [ 3 H]TRH to GH4C1 cells by ultraviolet illumination. The crosslinkage of [ 3 H]TRH is UV-dose dependent and is inhibited by an excess of unlabeled TRH. A 64 kDa protein is also detected on immunoblots using an antiserum raised against GH4C1 cell surface epitopes. In a closely related cell line (GH12C1) which does not bind [ 3 H]TRH, the 64 kDa protein cannot be demonstrated by [ 3 H]TRH crosslinking nor by immunoblotting. These findings indicate that the 64 kDa protein is a candidate for a TRH-receptor protein in GH4C1 cells

  19. Aspectos morfológicos da uretra de ratas após eletroestimulação do assoalho pélvico Morphological aspects of the urethra in female rats after electrical stimulation of the pelvic floor

    Directory of Open Access Journals (Sweden)

    Adriana Luciana Moreno Camargo

    2013-04-01

    Full Text Available OBJETIVO: Avaliar os efeitos da eletroestimulação (ES do assoalho pélvico na uretra de ratas. MÉTODOS: Quarenta ratas adultas foram distribuídas, randomicamente, em quatro grupos com dez animais cada: Ctrl - sem intervenção; Sham - não foi submetido a ES, recebeu um eletrodo dentro da vagina; Exp6 - submetido a seis sessões de ES do assoalho pélvico; e Exp12 - submetido a 12 sessões de ES do assoalho pélvico. Ao final do experimento, todos os animais foram anestesiados, e o terço médio da uretra foi retirado, fixado em líquido de Bouin e processado para estudo histomorfométrico. Alguns cortes foram corados pela hematoxilina e eosina, para descrição morfológica e morfométrica, e outros, pelo picrosirius red, para avaliação do colágeno total. As espessuras da camada muscular e do epitélio foram obtidas, nos 4 quadrantes da uretra, pela realização de 20 medições em cada animal. O número de vasos sanguíneos presentes na lâmina própria foi obtido nos quatro quadrantes, em uma área de 10³ mm² por quadrante, sendo as imagens obtidas pelo programa de análise de imagens AxioVision® REL 4.3 (Carl Zeiss. A proporção de colágeno e de fibras musculares foi obtida de duas imagens por quadrante, de cada lâmina da uretra corada pelo picrosirius red, com auxílio do programa Imagelab®. Os dados foram submetidos à análise de variância (ANOVA e ao teste de comparações múltiplas de Tukey-Kramer (pExp6==Ctrl==Sham; *pPURPOSE: To evaluate the effects of electrical stimulation (ES of the pelvic floor on the urethra of female rats. METHODS: Forty adult rats were divided at random into four groups of ten animals each: Ctrl - without intervention; Sham - not submitted to ES, but with an electrode inserted into the vagina; Exp6 - submitted to six sessions of ES of the pelvic floor, and Exp12 - submitted to 12 sessions of ES of the pelvic floor. At the end of the experiment, all animals were anesthetized and the middle third

  20. Imaging micro-glial/macrophage activation in spinal cords of experimental autoimmune encephalomyelitis rats by Positron Emission Tomography using the mitochondrial 18 kDa translocator protein radioligand [18F]DPA-714

    International Nuclear Information System (INIS)

    Abourbeh, Galith; Theze, Benoit; Dubois, Albertine; Tavitian, Bertrand; Boisgard, Raphael; Maroy, Renaud; Brulon, Vincent; Fontyn, Yoann; Dolle, Frederic

    2012-01-01

    Multiple sclerosis (MS) is an inflammatory demyelinating disease of the CNS. Activated micro-glia/macrophages play a key role in the immuno-pathogenesis of MS and its corresponding animal models, experimental autoimmune encephalomyelitis (EAE). Micro-glia activation begins at early stages of the disease and is associated with elevated expression of the 18 kDa mitochondrial translocator protein (TSPO). Thus, positron emission tomography (PET) imaging of micro-glial activation using TSPO-specific radioligands could be valuable for monitoring disease-associated neuro-inflammatory processes. EAE was induced in rats using a fragment of myelin basic protein, yielding acute clinical disease that reflects extensive spinal cord inflammation. Enhanced TSPO expression in spinal cords of EAE rats versus those of controls was confirmed by Western blot and immunohistochemistry. Biodistribution studies in control and EAE rats were performed using the TSPO radioligand [ 18 F]DPA-714 [N,N-diethyl-2-(2-(4-(2-fluoroethoxy)phenyl)-5,7-dimethylpyrazolo[1,5- a]pyrimidin-3-yl)acetamide]. At 1 h after injection, almost fivefold higher levels of [ 18 F]DPA-714 were measured in spinal cords of EAE rats versus controls. The specific binding of [ 18 F]DPA-714 to TSPO in spinal cords was confirmed in competition studies, using unlabeled (R,S)-PK11195 [(R,S)-N-methyl-N-(1-methylpropyl)-1-(2-chlorophenyl) - isoquinoline-3-carboxamide)] or DPA-714 in excess. MicroPET studies affirm that this differential radioactivity uptake in spinal cords of EAE versus control rats could be detected and quantified. Using [ 18 F]DPA-714, neuro-inflammation in spinal cords of EAE-induced rats could be visualized by PET, offering a sensitive technique for monitoring neuro-inflammatory lesions in the CNS and particularly in the spinal cord. In addition to current MRI protocols, this approach could provide molecular images of neuro-inflammation for detection, monitoring, and research in MS. (authors)

  1. Ação da melatonina sobre a dinâmica sanguínea de ratas prenhes e sobre a histogênese do baço e do timo da prole The action of melatonin on the blood dynamics of pregnant rats and on the histogenesis of the spleen and thymus of offspring

    Directory of Open Access Journals (Sweden)

    A.C.C. Araújo

    2013-04-01

    Full Text Available Investigou-se a influência da melatonina sobre o hemograma de ratas prenhes e dos filhotes e sobre a histogênese e morfometria do baço e do timo dos filhotes. A melatonina foi administrada na dose 0,5mg/kg de peso corporal, dissolvida em 0,1mL de etanol e diluída em 0,3mL de solução salina. Para análise do hematócrito, contagem total de hemácias e contagem total e diferencial dos leucócitos, amostras de sangue foram coletadas no sétimo, 14ºe 21ºdias de prenhez e aos 10 dias de nascimento dos filhotes. Cortes histológicos do baço e do timo da prole foram utilizados para histoquímica e morfometria. A ausência da melatonina promoveu alterações no hemograma apenas no terço final da gestação, sem interferir no hemograma dos filhotes, e induziu modificações morfológicas e morfométricas no timo e no baço nos primeiros dias de vida dos filhotes. Concluiu-se que a melatonina materna é importante para a modulação do hemograma em ratas prenhes e para o desenvolvimento normal do baço e do timo dos filhotes.We investigated the influence of melatonin on the hemogram of pregnant rats and puppies, and on the histogenesis and morphology of the spleen and thymus of puppies. Melatonin was administered at a dose 0.5mg/kgbody weight, dissolved in 0.1mL of ethanol and diluted in 0.3mL of saline. For hematocrit analysis, total erythrocyte count and total and differential leukocyte count, blood samples were collected on the 7th, 14th and 21st days of pregnancy and the offspring at 10 days of birth. Histological sections of spleen and thymus of the offspring were used for histochemistry and morphometry. The absence of melatonin promoted changes in blood count only in the final third of gestation, without interfering with the hemogram of the puppies, and induced morphological and morphometric changes in the thymus and spleen in the first days of life of the puppies. It was concluded that maternal melatonin is important for the modulation of

  2. Potencialização do efeito metemoglobinizante da dapsona em ratos pela N-acetilcisteína Potentiation of dapsone induced methemoglobinemia by N-acetylcysteine in rats

    Directory of Open Access Journals (Sweden)

    Natália Valadares de Moraes

    2008-03-01

    Full Text Available Dapsona (DDS (4,4'diaminodifenilsulfona, fármaco de escolha para o tratamento da hanseníase, freqüentemente induz anemia hemolítica e metemoglobinemia. A N-hidroxilação, uma de suas principais vias de biotransformação, é constantemente relacionada com a metemoglobinemia observada com o uso do fármaco. Com o objetivo de prevenir a hemotoxicidade induzida pela DDS, N-acetilcisteína, fármaco precursor de glutationa, foi administrada em associação com DDS em ratos machos Wistar pesando 220-240 g. Os animais foram anestesiados e o sangue coletado da aorta para determinação da concentração plasmática de DDS por CLAE, determinação dos níveis de metemoglobina e de glutationa eritrocitária por espectrofotometria, e avaliação de parâmetros bioquímicos e hematológicos. Os resultados obtidos mostraram que a N-acetilcisteína potenciou o efeito metemoglobinizante da dapsona devido ao aumento de sua concentração plasmática e conseqüente aumento da formação da N-hidroxilamina. Concluímos que as interações medicamentosas com a dapsona exigem estudos individualizados a fim de evitar os efeitos adversos do fármaco.Dapsone (DDS (4,4'diaminodiphenylsulfone, the drug of choice for the treatment of leprosy, frequently induces hemolytic anemia and methemoglobinemia. N-hydroxylation, one of the major pathways of biotransformation, has been constantly related to the methemoglobinemia after the use of the drug. In order to prevent the dapsone-induced hemotoxicity, N-acetylcysteine, a drug precursor of glutathione, was administered in combination with DDS to male Wistar rats, weighting 220-240 g. The animals were then anaesthetized and blood was collected from the aorta for determination of plasma DDS concentration by HPLC, determination of methemoglobinemia and glutathione by spectrophotometry, and for biochemical and hematological parameters. Our results showed that N-acetylcysteine enhanced dapsone-induced methemoglobinemia due to

  3. Selective affinity labeling of a 27-kDa integral membrane protein in rat liver and kidney with N-bromoacetyl derivatives of L-thyroxine and 3,5,3'-triiodo-L-thyronine

    International Nuclear Information System (INIS)

    Koehrle, J.R.; Rasmussen, U.B.; Rokos, H.; Leonard, J.L.; Hesch, R.D.

    1990-01-01

    125I-Labeled N-bromoacetyl derivatives of L-thyroxine and L-triiodothyronine were used as alkylating affinity labels to identify rat liver and kidney microsomal membrane proteins which specifically bind thyroid hormones. Affinity label incorporation was analyzed by ethanol precipitation and individual affinity labeled proteins were identified by autoradiography after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. Six to eight membrane proteins ranging in size from 17 to 84 kDa were affinity labeled by both bromoacetyl-L-thyroxine (BrAcT4) and bromoacetyl-L-triiodothyronine (BrAcT3). Affinity labeling was time- and temperature-dependent, and both reduced dithiols and detergents increased affinity labeling, predominantly in a 27-kDa protein(s). Up to 80% of the affinity label was associated with a 27-kDa protein (p27) under optimal conditions. Affinity labeling of p27 by 0.4 nM BrAc[125I]L-T4 was blocked by 0.1 microM of the alkylating ligands BrAcT4, BrAcT3, or 100 microM iodoacetate, by 10 microM concentrations of the non-alkylating, reversible ligands N-acetyl-L-thyroxine, 3,3',5'-triiodothyronine, 3,5-diiodosalicylate, and EMD 21388, a T4-antagonistic flavonoid. Neither 10 microM L-T4, nor 10 microM N-acetyltriiodothyronine or 10 microM L-triiodothyronine blocked affinity labeling of p27 or other affinity labeled bands. Affinity labeling of a 17-kDa band was partially inhibited by excess of the alkylating ligands BrAcT4, BrAcT3, and iodoacetate, but labeling of other minor bands was not blocked by excess of the competitors. BrAc[125I]T4 yielded higher affinity label incorporation than BrAc[125I]T3, although similar banding patterns were observed, except that BrAcT3 affinity labeled more intensely a 58,000-Da band in liver and a 53,000-55,000-Da band in kidney

  4. Avaliação da ação da Aroeira (Schinus terebinthifolius Raddi na cicatrização de feridas cirúrgicas em bexiga de ratos Evaluation of the aroreira (Schinus terebinthifolius Raddi in the healing process of surgical incision in the bladder of rats

    Directory of Open Access Journals (Sweden)

    Periguari Luiz Holanda de Lucena

    2006-01-01

    Full Text Available OBJETIVO: Avaliar a ação cicatrizante do extrato hidroalcoólico da aroeira em bexigas de ratos. MÉTODOS: Foram utilizados 40 ratos machos da linhagem Wistar, divididos em dois grupos de 20, denominados grupo aroeira (GA e grupo controle (GC. Todos foram submetidos à incisão abdominal mediana com cistotomia de 1 cm, seguida de cistorrafia em plano único com pontos separados de poliglactina 910 5-0 (Vicryl®. Após este procedimento, nos animais GA injetou-se 100mg por quilo de peso de extrato hidroalcoólico da aroeira na cavidade peritoneal e nos GC injetou-se 1 ml por quilo de peso de solução salina a 0,9% . Cada grupo foi dividido em dois sub-grupos de 10 animais de acordo com a data da morte: sub-grupo A3 e C3, sacrificados no 3º dia pós-operatório e sub-grupo A7 e C7, sacrificados no 7º dia. A parede, a cavidade abdominal e a sutura da bexiga foram avaliadas macroscopicamente. Amostras de tecido da bexiga foram retiradas e analisadas histologicamente, utilizando a coloração de Hematoxilina-Eosina (HE e tricrômio de Masson. RESULTADOS: Na análise macroscópica observou-se infecção na incisão cirúrgica em três ratos do GC e em um do GA, e aderências peritoneais em 29 ratos do GC controle e 17 no GA. A avaliação microscópica mostrou processo inflamatório agudo mais severo no 3° (p=0,045 e no 7° dia (p=0,002. Evidenciou-se ainda diferença estatística nos parâmetros utilizados para a avaliação histológica da cicatrização da bexiga nas variáveis colagenização (p = 0.001, reepitelização (p = 0,046 e neoformação (p = 0 nos subgrupos GC e GA no 3º dia e na variável neoformação vascular (p=0,001 no subgrupo do 7º dia. CONCLUSÃO: O uso de extrato hidroalcoólico de aroeira mostrou efeito cicatrizante favorável nas cistotomias em ratos.PURPOSE: To evaluate the hydroalcoholic aroeira extract in the healing process of surgical incisions in the bladder of rats in an experimental comparative study. METHODS

  5. Regeneração do fígado de ratos após oclusão parcial da drenagem venosa hepática Hepatic regeneration after parcial oclusion of hepatic vein drainage in rats

    Directory of Open Access Journals (Sweden)

    Luiz Roberto Farion de Aguiar

    2009-06-01

    Full Text Available INTRODUÇÃO: A regeneração hepática é um mecanismo para superar a perda de tecido funcional do fígado. Este processo é estudado através de diferentes métodos. OBJETIVO: Avaliar o efeito da oclusão parcial da drenagem venosa hepática sobre a regeneração do fígado remanescente de ratos submetidos à hepatectomia parcial. MÉTODO: Foram colhidas biópsias de fígado em 30 ratos Wistar machos, e a seguir realizada hepatectomia a dois terços. Os animais foram divididos em três grupos: um grupo controle e dois grupos de estudo, submetidos a diferentes graus de estenose da veia hepática direita. Após 96 horas do estímulo para regeneração hepática, todos submeteram-se à outra biópsia hepática. Analisaram-se os fragmentos por imunoistoquímica para os marcadores Ki-67 e fator de von Willebrand. Para a leitura das amostras utilizou-se o sistema SAMBA 4000. A deposição de colágeno foi avaliada pela coloração tricrômico de Masson. RESULTADOS: A proliferação celular dos animais submetidos à hepatectomia parcial e estenose moderada ou severa da veia hepática direita persistiu mais elevada quando comparada ao grupo controle. O Índice de Marcação para o Ki-67 foi significativamente mais elevado após a hepatectomia nos grupos submetidos à oclusão parcial da veia hepática, tanto moderada quanto severa. A expressão de fator de von Willebrand estava diminuída após a hepatectomia parcial nos três grupos. Houve pouco depósito de colágeno no tecido hepático nos animais dos dois grupos com estenose da veia hepática direita. CONCLUSÃO: A oclusão parcial da drenagem venosa hepática em ratos submetidos à hepatectomia parcial prolonga o tempo de proliferação de células hepáticas quando comparado aos animais com veias de calibre normal. Como consequência, também houve atraso na restauração da matriz extracelular e na formação de novos vasos sinusoidais.BACKGROUND: Hepatic regeneration is a mechanism to overcome

  6. Evaluation of the energetic equivalence of goat manure biogas; Avaliacao da equivalencia energetica do biogas de esterco de caprinos

    Energy Technology Data Exchange (ETDEWEB)

    Canafistula, Francisco Jose Firmino; Carvalho, Paulo Cesar Marques de [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Engenharia Eletrica], e-mails: firmino@ufc.br, carvalho@dee.ufc.br

    2008-07-01

    The present paper shows the results of a research about a new production system model based on goats; part of the animals manure is used for biogas production. The biogas is used as fuel for water pumping for the irrigation of the animals pasture. For the viability of the project, a photovoltaic powered electrified fence was used. Additional to the positive results of sustainability, innovative solutions were developed for sizing, optimization and costs reduction by the use of digesters in small rural communities of the semi-arid of the Brazilian Northeast Region. (author)

  7. Evaluation of the absorbed dose in odontological computerized tomography; Avaliacao da dose absorvida em tomografia computadorizada odontologica

    Energy Technology Data Exchange (ETDEWEB)

    Legnani, Adriano; Schelin, Hugo R.; Rocha, Anna Silvia P.S. da, E-mail: schelin@utfpr.edu.b, E-mail: anna@utfpr.edu.b [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Khoury, Helen J., E-mail: khoury@ufpe.b [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2011-10-26

    This paper evaluated the absorbed dose at the surface entry known as 'cone beam computed tomography' (CBCT) in odontological computerized tomography. Examination were simulated with CBCT for measurements of dose. A phantom were filled with water, becoming scatter object of radiation. Thermoluminescent dosemeters were positioned on points correspondent to eyes and salivary glands

  8. Evaluation of the absorbed dose in odontological computerized tomography; Avaliacao da dose absorvida em tomografia computadorizada odontologica

    Energy Technology Data Exchange (ETDEWEB)

    Legnani, Adriano; Schelin, Hugo R; Rocha, Anna Silvia P.S. da, E-mail: schelin@utfpr.edu.b, E-mail: anna@utfpr.edu.b [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Khoury, Helen J., E-mail: khoury@ufpe.b [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2011-10-26

    This paper evaluated the absorbed dose at the surface entry known as 'cone beam computed tomography' (CBCT) in odontological computerized tomography. Examination were simulated with CBCT for measurements of dose. A phantom were filled with water, becoming scatter object of radiation. Thermoluminescent dosemeters were positioned on points correspondent to eyes and salivary glands

  9. Evaluation of elemental composition of clays from Campos Gerais (MG); Avaliacao da composicao elementar de argilas de Campos Gerais (MG)

    Energy Technology Data Exchange (ETDEWEB)

    Martins, Joao P.M.; Maduar, Marcelo F.; Silva, Paulo S.C da, E-mail: jpmm0697@hotmail.com [Instituto de Pesquisas Energeticas e nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    There are numerous applications given to clays including oil and water adsorbent, ceramic, whitening of beverages, porcelain, waste treatment, organic carrier molecules in cosmetics and pharmaceuticals, support for catalysts. In the pharmaceutical industry, the clays are used as excipients, diluents, desiccants, emulsifiers, to mask undesirable flavors, isotonic agent such as charger and delivery of active substances. These characteristics have contributed to the expansion of the search for applications of clay minerals in the cosmetic industry. The aim of this study was to determine the elemental composition of clays from Campos Gerais, Minas Gerais, with a view to their applicability in the production of cosmetics. The elements As, Ba, Br, Ce, Cl, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Na, Nd, Rb, Sb, Sc, Sm, Ta, Tb, Th,U, Yb and Zn were determined by neutron activation analysis and radionuclide activity concentration of {sup 226}Ra, {sup 228} Ra, {sup 210}Pb and {sup 40}K were determined by gamma spectrometry. It was verified that the activity concentration of radionuclides was in the same concentration as the global average, indicating that these samples do not present a risk of increased radiation exposure. The concentration of most elements determined is less than or equal to the overall mean concentrations, indicated by the values of Continental Upper Crust. (author)

  10. Evaluation of welding by MIG in martensitic stainless steel; Avaliacao da soldagem pelo processo MIG em aco inoxidavel martensitico

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, M.A. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil); Mariano, N.A.; Marinho, D.H.C. Marinho, E-mail: neideaparecidamariano@gmail.co [Universidade Federal de Alfenas (UNIFAL), Pocos de Caldas, MG (Brazil)

    2010-07-01

    This work evaluated structure's characterization and mechanical properties after the welding process of the stainless steel CA6NM. The employed welding process was the metal active gas with tubular wire. The control of the thermal cycle in the welding process has fundamental importance regarding the properties of the welded joint, particularly in the thermally affected zone. The mechanical properties were appraised through impact resistance tests and the hardness and microstructure through metallographic characterization and Ray-X diffraction. The parameters and the process of welding used promoted the hardness and toughness appropriate to the applications of the steel. Welding energy's control becomes an essential factor that can affect the temperature of carbide precipitation and the nucleation of the retained austenite in the in the region of the in the thermally affected zone. (author)

  11. Evaluation of kerma rate in radioactive waste disposal; Avaliacao da taxa de kerma em deposito de rejeitos radioativos

    Energy Technology Data Exchange (ETDEWEB)

    Rosa, Rodolfo O.; Silva, Joao C.P.; Santos, Joao R. dos, E-mail: oliveira@ien.gov.br, E-mail: jcarlos@ien.gov.br, E-mail: regis@ien.gov.br [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2014-07-01

    This study aims to assess the progression of kerma rate levels in the air due to the increase of collection, storing and storage of radioactive waste in the new building (after expansion) of the radioactive waste disposal (RWD) of the Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Brazil. This review is carried out every six months at IEN with thermoluminescent dosimeter lithium fluoride LiF: Mg, Cu, P (TLD-100H). Here are the average values of kerma rate for the period 2008- 2012. In this context, the methodology used for selection and choices of detectors used in dosimeters is presented. The detectors were chosen through homogeneity criteria of the pack, standardization factor and coefficient of variation (CV%). The monitoring points and the exposure time of the detectors are chosen considering various factors, including the rate of occupation and indoor and outdoor positions to RWD. These evaluations showed that the contribution of the new waste disposal in increasing kerma rate of IEN, has proved to be insignificant, that is, the presence of RWD does not contribute to increased environmental kerma rate in the region around this installation.

  12. Evaluation of radioiodinetherapy protocol for patients with Plummer disease; Avaliacao do protocolo da radioiodoterapia para pacientes com doenca de Plummer

    Energy Technology Data Exchange (ETDEWEB)

    Mendonca, Keyla Mary Cavalheiro; Antonio Filho, Joao; Brandao, Simone Cristina Soares, E-mail: keylamcm@yahoo.com.br, E-mail: jaf@ufpe.br, E-mail: simonecordis@yahoo.com.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil); Santos, Marcus Aurelio P. dos, E-mail: masantos@cnen.gov.br [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil)

    2014-07-01

    The disease Plummer (DP) is generated by the thyroid cells that function autonomously, independent the rest of the organ parenchyma. The DP presents a prevalence of 1% to 9%, higher in regions with iodine deficiency, affecting six women for every man. The study was conducted at the Department of Nuclear Medicine of Universidade Federal de Pernambuco (UFPE) from {sup 99m}Tc data capture and mass existing in the thyroid records of 54 patients with PD who underwent radioiodine therapy with fixed activities 1110 megabecquerels (MBq) from 2004 to 2008. For capitation of {sup 99m}Tc from a group of 22 patients were used 5 ml syringes, measured by a Capintec CRC-7 model activimeter, having scintigraphy after 20 minutes and results in a region of interest. Mass evaluation was based on method of palpation of the thyroid, considering the mass standard. It is observed that 68.2% of patients received more activity than needed, 31.8% were less active, and none received activity in accordance with your needs. There was a statistically significant difference (p <0.05) between the fixed activities and activities computed for all patients, according to Chi-square test for independence with 21 degrees of freedom. The cure rate of a group of 32 patients was 75% after one year of follow-up. It is suggested that the RIT is preceded by an assessment of the maximum percentage of uptake and thyroid mass.

  13. Evaluation of the dispersion of oil refinery liquid effluent; Avaliacao da dispersao de efluente liquido de refinaria de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Mariano, Adriano Pinto; Silva, Ariovaldo Jose da; Oliveira, Valdenilson Jose Alves de; Angelis, Dejanira de Franceschi de [Universidade Estadual Paulista (UNESP), Rio Claro, SP (Brazil). Dept. de Bioquimica e Microbiologia. Inst. de Biociencias], e-mail: adrianomariano@yahoo.com.br

    2010-07-15

    In this paper, two techniques to evaluate the dispersion of the effluent of an oil refinery are presented. Thus, the dispersion plume was characterized by field measurements of electrical conductivity and by computational simulation (Cormix simulator). An oil refinery whose effluent is discharged on the Atibaia river (Paulinia/SP) is taken as study case. The behavior of the effluent was evaluated until 1000 m after the discharge. The results show that the measurement of electrical conductivity is a suitable technique to evaluate the dispersion of oil refinery wastewaters, since they have high conductivity and for this reason there is a strong contrast between the values of the wastewater and the ambient background. Furthermore, other water quality parameters had a dispersion behavior similar to that shown by the conductivity. The dispersion plume generated by the computational simulator showed high concordance with the field data. In this manner, computational simulation can be a useful tool to evaluate the dispersion of discharges considering hypothetical scenarios, as well as to design the discharge channel. (author)

  14. Cementing quality evaluation with ultrasonic logs in fiberglass casings; Avaliacao da qualidade do cimento em revestimentos de fibra de vidro

    Energy Technology Data Exchange (ETDEWEB)

    Campos, Wellington; Lazaro, Andre F. [PETROBRAS, Rio de Janeiro, RJ (Brazil)

    2008-07-01

    The sonic and ultrasonic profiles are used as the main tools in assessing the cementing quality between formation and casing in oil wells. This assessment is important, because, if there is a failure in the primary cementing, both the structural integrity and zone isolation will be compromised. The sonic profiles are based on the acoustic energy attenuation in casing, cement and formation, while the ultrasonic profiles are based on the resonance of the wave pulse within the media where they travel (casings, cement and formation). The attenuation and resonance are due to the difference in the way the wave travel within these media. The acoustic impedance is the quantification of this difference, determining the refraction and reflection between the environments, and wave attenuation as well. In steel casings, this difference is meaningful, allowing the captured signals (reflected pulses) to be interpreted as good adhesion between cement and casing, or a lack of adhesion at some interval. In fiber glass casings, the impedance contrast between glass and cement is small and not detectable with the CBL/VDL sensors. The CBL/VDL tools provide an inefficient assessment of the quality of the cementing. The ultrasonic profile does not have this problem, theoretically. The goal of this work is to demonstrate and recommend the ultrasonic tool as the main instrument to assess the cementation quality in fiber glass casings. (author)

  15. Evaluation of the ocular protection for low intensity therapeutic lasers; Avaliacao da protecao ocular para lasers terapeuticos em baixa intensidade

    Energy Technology Data Exchange (ETDEWEB)

    Cordon, Rosely

    2003-07-01

    The low intensity laser therapy (LILT) has been extensively used in medicine and dentistry presenting positive effects. However, the laser radiation can also cause adverse effects. Due to the ocular focalization property, in the wavelength from 400 to 1400 nm, the retina is more susceptible to damage by radiation than any other part of the human body. Then, the ocular protection is frequently emphasized. This protection must attenuate the radiation to a safe level. The International Electrotechnical Commission (IEC) standard IEC 60825-1 suggests safety requirements for medical laser equipment, including the ocular protection, based on maximum permissible exposure levels. The Brazilian legislation adopts a corresponding IEC standard, the NBR IEC 601.2.22, for safety requirements. The aim of this study was to analyze the adequacy of the ocular protectors furnished by four laser equipment manufacturers, commercially available in Brazil, commonly used for LILT. For this purpose, the laser equipment and the respective ocular protectors were characterized. The adequacy was verified according to the IEC standards. It was found, among other results, ocular protectors attenuating to safe levels the radiation emitted by the respective laser equipment, however, presenting inadequate visual transmission. Inefficient protection and protection indicated in cases where they were not necessary were also observed. (author)

  16. Evaluation of external exposure in a radioactive waste deposit; Avaliacao da exposicao externa em um deposito de rejeitos radioativos

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Sergio Quinet de; Pereira, Wagner de Souza; Py Junior, Delcy de Azevedo; Dores, Luis Augusto de Carvalho Bresser; Dantas, Marcelino Vicente de Almeida; Silva, Ana Claudia Antunes; Garcia Filho, Oswaldo, E-mail: wspereira@inb.gov.b, E-mail: sergioquinet@inb.gov.b, E-mail: delcy@inb.gov.b, E-mail: luisdores@inb.gov.b, E-mail: marcelino@inb.go.b, E-mail: anasilva@inb.gov.b, E-mail: ogarcia@inb.gov.b [Industrias Nucleares do Brasil (UTM/INB), Pocos de Caldas, MG (Brazil). Unidade de Tratamento de Minerio

    2011-10-26

    The ore treatment unit (OTU) of Industrias Nucleares do Brasil (INB), located at Caldas municipality - Minas Gerais, Brazil, posses a radioactive deposit, coming mainly from the chemical processing of monazite sands for obtention of 'rare earths'. The main components of these waste are the 'torta II (thorium rich residues) and the 'mesothorium' ({sup 228}Ra rich residues) - conditioned in steel drums (200 liters), plastic pumps (100 liters) or underground silos. These loaders are deposited in waste loading warehouses existent at the OTU and periodic evaluations of the external exposure rates (mR/h) are part of the 'Programa de Monitoracao Radiologica Ocupacional' of the unit. This paper presents a brief history of origins of this waste deposit and the material found there, and also the result of a routine monitoring of the external exposure rates

  17. Evaluation of integrity of radiation sources of nuclear gauges; Avaliacao da integridade de fontes radioativas de medidores nucleares

    Energy Technology Data Exchange (ETDEWEB)

    Torohate, Wiclif Francisco

    2016-11-01

    Nuclear equipment meters are mainly used in the industry in quality control and process control. The principle of operation consists in a shielded radioactive source together with a radiation detector such that the radiation interacts with the material to be analyzed before reaching the detector, providing real time data. Can be as their fixed and mobile mobility, the unique properties of ionizing radiation are used in three basic modes, transmission, backscatter or dispersion or induced (reactive). With the advancement and technological modernization in the world, the demand for nuclear gauges becomes increasingly larger. Currently in Brazil there are about 465 process control plants and 21 portable systems and Mozambique about 45 facilities using nuclear gauges. This font registration is done through a process called source inventory that allows also to know the category of the source, the danger or risk to human health that the source offers. The handling of this equipment requires personnel, certified, skilled and well trained in radiation protection area in accordance with the requirements of the various CNEN Rules. Due to the presence of radioactive source and because these devices are used by workers risk because there external radiation. In this context, we made the smear test in two fixed meters from the IRD industry laboratory, which determines the integrity of the source package, mandatory item in periodic integrity testing of the radiation source of this type of device. A set of procedures is made for its implementation as an evaluation of the radiological risk by radiological survey. It was intended to contribute to the learning handling and safe use of these meters. (author)

  18. External exposure assessment in dwelling built with phosphogypsum; Avaliacao da exposicao externa em residencia construida com fosfogesso

    Energy Technology Data Exchange (ETDEWEB)

    Villaverde, Freddy Lazo

    2008-07-01

    In this study it was evaluated the viability of the use of phosphogypsum plates as a building material in the dwelling construction. Thus, the effective dose due to external gamma exposure was assessed through the {sup 226}Ra, {sup 232}Th, {sup 210}Pb e {sup 40}K activity concentration in phosphogypsum plates. Samples of this material were analyzed by high resolution gamma spectrometry for their natural radionuclide activity concentration. The radium equivalent activity and extern ai and inter nai hazard indices were also calculated. The plates were made with phosphogypsum from fertilizer industries located in Cajati, Cubatao and Uberaba. The samples were identified according to phosphogypsum origin, Cajati (CA), Cubatao (CT) and Uberaba (UB). The activity concentrations results varied from 15.9 to 392 Bq kg{sup -1} for {sup 226}Ra, 26.1 to 253 Bq kg{sup -1} for {sup 232}Th, and 27.4 to 852 Bq kg{sup -1} for {sup 210}Pb. The results of {sup 40}K were lower than 81 Bq kg{sup -1}. The annual effective dose was obtained through the dosimetric model with reference standard room concept, the results were 0.02 mSv y{sup -1} for a house built with phosphogypsum from origin CA, 0.2 mSvy{sup -1} for CT phosphogypsum and 0.14 mSvy{sup -1} for UB phosphogypsum, everything the effective doses were below 1 mSvy{sup -1}, an annual effective dose limit for public exposure by International Commission on Radiological Protection. (author)

  19. A Comparative study for striatal-direct and -indirect pathway neurons to DA depletion-induced lesion in a PD rat model.

    Science.gov (United States)

    Zheng, Xuefeng; Wu, Jiajia; Zhu, Yaofeng; Chen, Si; Chen, Zhi; Chen, Tao; Huang, Ziyun; Wei, Jiayou; Li, Yanmei; Lei, Wanlong

    2018-04-16

    Striatal-direct and -indirect Pathway Neurons showed different vulnerability in basal ganglia disorders. Therefore, present study aimed to examine and compare characteristic changes of densities, protein and mRNA levels of soma, dendrites, and spines between striatal-direct and -indirect pathway neurons after DA depletion by using immunohistochemistry, Western blotting, real-time PCR and immunoelectron microscopy techniques. Experimental results showed that: 1) 6OHDA-induced DA depletion decreased the soma density of striatal-direct pathway neurons (SP+), but no significant changes for striatal-indirect pathway neurons (ENK+). 2) DA depletion resulted in a decline of dendrite density for both striatal-direct (D1+) and -indirect (D2+) pathway neurons, and D2+ dendritic density declined more obviously. At the ultrastructure level, the densities of D1+ and D2+ dendritic spines reduced in the 6OHDA groups compared with their control groups, but the density of D2+ dendritic spines reduced more significant than that of D1. 3) Striatal DA depletion down-regulated protein and mRNA expression levels of SP and D1, on the contrary, ENK and D2 protein and mRNA levels of indirect pathway neurons were up-regulated significantly. Present results suggested that indirect pathway neurons be more sensitive to 6OHDA-induced DA depletion. Copyright © 2018 Elsevier Ltd. All rights reserved.

  20. Effects of the condylar process fracture on facial symmetry in rats submitted to protein undernutrition Efeitos da fratura do processo condilar na simetria facial em ratos submetidos à desnutrição protéica

    Directory of Open Access Journals (Sweden)

    Lucimar Rodrigues

    2011-04-01

    Full Text Available PURPOSE: To investigate the facial symmetry of rats submitted to experimental mandibular condyle fracture and with protein undernutrition (8% of protein by means of cephalometric measurements. METHODS: Forty-five adult Wistar rats were distributed in three groups: fracture group, submitted to condylar fracture with no changes in diet; undernourished fracture group, submitted to hypoproteic diet and condylar fracture; undernourished group, kept until the end of experiment, without condylar fracture. Displaced fractures of the right condyle were induced under general anesthesia. The specimens were submitted to axial radiographic incidence, and cephalometric mensurations were made using a computer system. The values obtained were subjected to statistical analyses among the groups and between the sides in each group. RESULTS: There was significative decrease of the values of serum proteins and albumin in the undernourished fracture group. There was deviation of the median line of the mandible relative to the median line of the maxilla, significative to undernutrition fracture group, as well as asymmetry of the maxilla and mandible, in special in the final period of experiment. CONCLUSION: The mandibular condyle fracture in rats with proteic undernutrition induced an asymmetry of the mandible, also leading to consequences in the maxilla.OBJETIVO: Investigar a simetria facial de ratos submetidos à fratura experimental de côndilo mandibular e com desnutrição protéica (8% de proteína por meio de mensurações cefalométricas. MÉTODOS: 45 ratos Wistar adultos foram distribuídos em três grupos: grupo fraturado, submetido a fratura condilar sem alteração na dieta; grupo fraturado desnutrido, submetido a dieta hipoprotéica e fratura condilar; grupo desnutrido, mantido até o final do experimento, sem fratura condilar. Fraturas com desvio foram feitas no côndilo direito com anestesia geral. Os espécimes foram submetidos à incidência radiogr

  1. Effect of the nandrolone decanoate on the efficiency of spermatogenesis of sedentary rats and rats subjected to physical training = Efeito do decanoato de nandrolona na eficiência da espermatogênese de ratos sedentários e submetidos ao treinamento físico

    Directory of Open Access Journals (Sweden)

    Marcela de Paiva Foletto

    2010-01-01

    Full Text Available Spermatogenesis is a complex, highly organized and coordinated process that results in the production of male gametes. This process is influenced by a number of drugs that enhance physical performance, which have been used mainly by young people, athletes or not, seeking to gain athletic performance or only a prominent social position. The objective of this study was to evaluate the effects of the anabolic steroid nandrolone decanoate on the efficiency of spermatogenesis in sedentary rats and rats subjected to physical training. Twenty-four male rats were divided into four experimental groups: sedentary control, sedentary treated, trained control and trained treated. Treated animals received intramuscular injection of nandrolone decanoate (0.5 mg kg-1 body weight during the last four weeks of physical training. The training program consisted of running on a programmable ergometer treadmill, tailored to train eight rats simultaneously, for nine weeks. We evaluated the morphological and morphoquantitative profiles of the male reproductive system. The results showed that the treatment causes a reduction in the efficiency of spermatogenesis; however, when associated with physical training, this compensates for the anabolic action, keeping the process of spermatogenesis within normality.A espermatogênese é um processo complexo, altamente organizado e coordenado, que resulta na produção dos gametas masculinos. Este processo é influenciado por uma série de drogas potencializadoras do desempenho físico, as quais têm sido utilizadas principalmente, por jovens, atletas ou não, que buscam adquirir maior desempenho esportivo ou mesmo apenas uma posição de destaque na sociedade. O objetivo deste estudo foi avaliar os efeitos do anabolizante esteroide decanoato de nandrolona na eficiência da espermatogênese de ratos sedentários e submetidos ao treinamento físico. Foram utilizados 24 ratos machos divididos em quatro grupos experimentais: sedent

  2. Influência do veículo na eficácia da reposição de potássio em ratos hipocalêmicos Vehicle influence on potassium replacement effectiveness in hypokalemic rats

    Directory of Open Access Journals (Sweden)

    Márcio Petenusso

    2009-09-01

    Full Text Available INTRODUÇÃO: Pacientes após cirurgia cardíaca são comumente tratados com diuréticos para controle de volume plasmático. A preocupação de distúrbios hipocalêmicos em adultos antes, durante ou após a cirurgia já foi ressaltada anteriormente, visto o risco de arritmias cardíacas. Clinicamente, a diluição da solução de potássio (K+ para administração por via intravenosa, em situações que requerem a sua reposição é realizada utilizando-se soro fisiológico (SF ao invés de soro glicosado 5% (SG5%, possivelmente em vista de poder ocorrer estimulação da secreção de insulina, que interferiria sobre a qualidade da reposição de K+. Porém, não está comprovado experimentalmente se o SF e SG5% poderiam realmente interferir na qualidade da reposição de potássio em ratos com hipocalemia. OBJETIVO: Analisar a influência da reposição de K+ diluído em diferentes veículos sobre as concentrações plasmáticas de K+([K+]p em ratos submetidos a hipocalemia induzida por furosemida. MÉTODOS: Ratos Wistar adultos foram divididos em quatro grupos: K++SF, K++SG, SF e SG. Foi realizada a canulação da veia jugular para reposição e da veia femoral para coleta de sangue. O diurético furosemida na dose de 50mg/kg foi usado para induzir hipocalemia, foi analisado nível plasmático de potássio 24 h antes da injeção de furosemida, 24 h pós-indução e 30 minutos pós-reposição. RESULTADOS: Os níveis da [K+] pós-injeção de furosemida sofreram redução, comparado aos valores basais (pré-furosemida em todos os grupos. Entretanto, os níveis [K+] retornaram aos valores basais tanto nos grupos que receberam K++SF ou K++SG, o que não foi observado nos grupos que receberam apenas SF e SG. Quanto ao Na+ plasmático, somente o grupo K+SF apresentou aumento após reposição. CONCLUSÃO: A reposição de K+ diluído tanto em SF quanto SG parece não afetar a qualidade da reposição de K+ plasmático em ratos

  3. The influence of cigarette smoke inhalation on bone density: a radiographic study in rats Influência da fumaça de cigarro na densidade óssea: estudo radiográfico em ratos

    Directory of Open Access Journals (Sweden)

    João Batista César-Neto

    2005-03-01

    Full Text Available The aim of this study was to evaluate the influence of cigarette smoke inhalation (CSI and its cessation on tibiae bone quality. Forty-one male Wistar rats were randomly assigned to one of the following groups: Group 1 - control (n = 14, Group 2 - 3 months of CSI and 2 months without exposure to CSI (n = 12, and Group 3 - 5 months of CSI (n = 15. At the end of the experimental period, the animals were sacrificed, the tibiae removed and immediately radiographed for photodensitometric analysis. The results showed that continuous exposure to cigarette smoke promoted a significantly reduced bone density (p 0.05. Thus, within the limits of the present study, it can be concluded that CSI may affect tibiae bone quality, and CSI cessation results in a return towards the level of the control group.O objetivo deste estudo foi avaliar a influência da inalação da fumaça de cigarro (IFC e o efeito de sua interrupção na qualidade óssea da tíbia. Quarenta e um ratos Wistar machos foram aleatoriamente designados a um dos seguintes grupos: Grupo 1 - controle (n = 14, Grupo 2 - 3 meses de IFC e 2 meses sem exposição à fumaça (n = 12 e Grupo 3 - 5 meses de IFC (n = 15. Ao final do período experimental, os animais foram sacrificados, as tíbias removidas e imediatamente radiografadas para a análise fotodensitométrica. Os resultados mostraram que a exposição contínua à fumaça de cigarro promoveu uma significante redução na densidade óssea (p 0,05. Portanto, dentro dos limites do presente estudo, pode-se concluir que a IFC pode influenciar a qualidade óssea da tíbia e que a interrupção da inalação parece reverter esse efeito negativo resultando numa densidade óssea semelhante à do grupo controle.

  4. Effect of lincomycin treatment of root surface in dental replantation: a study in rats = Efeito da lincomicina como tratamento da superfície radicular no reimplante de dentes: estudo em ratos

    Directory of Open Access Journals (Sweden)

    Silva, Daniela Nascimento

    2009-01-01

    Full Text Available Objetivo: Estudos sobre antibióticos usados topicamente na superfície radicular de dentes avulsionados buscam avaliar suas propriedades anti-reabsortivas que favoreçam o reparo do ligamento periodontal e o restabelecimento da articulação dentoalveolar. Neste mesmo propósito, esta pesquisa avaliou o efeito da lincomicina na superfície radicular, antes do reimplante. Metodologia: Dez ratos foram distribuídos em dois grupos de 05 animais (I – controle e II – experimental. O incisivo superior direito dos animais foi extraído e estocado em leite por 30 min. Em seguida, no grupo I, os dentes foram irrigados com soro fisiológico e reimplantados; no grupo II, os incisivos foram imersos em lincomicina por 5 min, antes do reimplante. Os animais foram eutanasiados no 21º dia pós-operatório e a hemimaxila direita submetida a cortes histológicos, corados em HE, para avaliação microscópica. Resultados: No grupo I, o ligamento periodontal apresentou-se disperso, com infiltrado linfoplasmocitário moderado a intenso e a articulação dentoalveolar não foi restabelecida; no grupo II, notou-se completo reparo do ligamento, poucas células inflamatórias e pequenas áreas de reabsorção inflamatória e anquilose. Conclusões: o uso tópico da lincomicina no tratamento da superfície radicular promoveu o reparo do ligamento periodontal, reduziu o processo inflamatório, mas ainda foram observadas áreas de reabsorção inflamatória e anquilose

  5. Expression and distribution of connexin 32 in rat liver with experimentally induced fibrosis Expressão e distribuição da conexina 32 em fígados de ratos com fibrose induzida experimentalmente

    Directory of Open Access Journals (Sweden)

    Alexandro dos S. Rodrigues

    2009-04-01

    Full Text Available The connexin 32 (Cx32 is a protein that forms the channels that promote the gap junction intercellular communication (GJIC in the liver, allowing the diffusion of small molecules through cytosol from cell-to-cell. Hepatic fibrosis is characterized by a disruption of normal tissue architeture by cellular lesions, and may alter the GJIC. This work aimed to study the expression and distribution of Cx32 in liver fibrosis induced by the oral administration of dimethylnitrosamine in female Wistar rats. The necropsy of the rats was carried out after five weeks of drug administration. They presented a hepatic fibrosis state. Sections from livers with fibrosis and from control livers were submitted to immunohistochemical, Real Time-PCR and Western-Blot analysis to Cx32. In fibrotic livers the Cxs were diffusely scattered in the cytoplasm, contrasting with the control livers, where the Cx32 formed junction plaques at the cell membrane. Also it was found a decrease in the gene expression of Cx32 without reduction in the protein quantity when compared with controls. These results suggest that there the mechanism of intercellular communication between hepatocytes was reduced by the fibrotic process, which may predispose to the occurrence of a neoplastic process, taken in account that connexins are considered tumor suppressing genes.A conexina 32 (Cx32 é uma proteína que constitui os canais que promovem as comunicações intercelulares via junções comunicantes (CIJC no fígado, permitindo difusão de pequenas moléculas citoplasmáticas de uma célula à outra. A fibrose hepática caracteriza-se pela alteração da arquitetura normal do fígado e podem alterar as CIJCs. O objetivo deste trabalho foi estudar a expressão e distribuição de Cx32 na fibrose hepática. O objetivo do presente trabalho foi estudar a expressão e distribuição da Cx32 em fígados com fibrose induzida pela administração oral de dimetilnitrosamina em fêmeas de ratos Wistar. A

  6. Epidermal growth factor reactivity in rat milk

    DEFF Research Database (Denmark)

    Raaberg, Lasse; Nexø, Ebba; Tollund, L

    1990-01-01

    whey elutes as a broad peak corresponding to a Stokes radius of 4.0 nm (an approximate molecular weight of 80 kDa). Almost no 6 kDa EGF is present. Judged by gel filtration of whey pre-incubated with 125I-EGF (6 kDa), no binding protein for EGF is present in rat whey. When rat milk is incubated...

  7. Synthesis and PET evaluation of the translocator protein (18 kDa) (T.S.P.O.) ligand [{sup 11}C]D.P.A.-715 in rat and non-human primate

    Energy Technology Data Exchange (ETDEWEB)

    Creelman, A.; Mcgregor, I.; Kassiou, M. [Sydney Univ., NSW (Australia); Thominiaux, C.; Chauveau, F.; Kuhnast, B.; Boutin, H.; Hantraye, P.; Tavitian, B.; Dolle, F. [Service Hospitalier Frederic Joliot 91 - Orsay (France); Fulton, R.; Henderson, D. [RPAH, NSW (Australia); Selleri, S. [Firenze Univ. (Italy)

    2008-02-15

    The translocator protein (18 kDa) (T.S.P.O.), formerly known as the peripheral benzodiazepine receptor (P.B.R.), is over expressed upon micro-glial activation. This study involved the evaluation of the pyrazolo-pyrimidine D.P.A.-715 (T.S.P.O. Ki = 16.4 nM) in behavioural studies and the radiolabelled form, [{sup 11}C]D.P.A.-715, in healthy non-human primate and A.M.P.A.-lesioned rats as a model of activated micro-glia using PET. The in vivo anxiolytic effects of D.P.A.-715 were assessed using the social interaction test which represents social anxiety in humans. [{sup 11}C]D.P.A.-715 was prepared using [{sup 11}C]CH{sub 3}I as the labelling intermediate from the phenolic precursor of D.P.A.-715 using T.B.A.H. and D.M.F. followed by H.P.L.C.. The non-human primate distribution studies were performed using a clinical PET scanner, and A.M.P.A.-lesioned rats using micro PET. Blocking studies were conducted using P.K.11195 (5 mg/kg).In the social interaction test a significant overall effect for the duration of time spent in general investigation, adjacent lying and rearing was observed. Post hoc analysis revealed a significantly greater time spent in general investigation and adjacent lying in the 20 mg/kg D.P.A.-715 treatment group compared to vehicle treated rats. The average non-decay corrected radiochemical yield of [{sup 11}C]D.P.A.-715 was 0.27 {+-} 0.05% with an average specific activity of 16.32 {+-} 4.01 GBq/mmol. The PET distribution studies revealed poor brain uptake. Pre-treatment with P.K.1195 resulted in no change of in the uptake of the radioligand, which suggests that brain uptake is representative of non-specific binding. In agreement with these results, the brain uptake in the A.M.P.A. lesioned model, depicted no significant differences between the lesioned striatum and the non-lesioned contralateral striatum. Although D.P.A.-715 does possess anxiolytic properties in vivo, [{sup 11}C]D.P.A.-715 does not possess the required properties for further

  8. Efeitos da radiação laser de dióxido de carbono em tecido ósseo: estudo macroscópico em ratos Effect of carbon dioxide laser radiation on osseous tissue: macroscopic study in rats

    Directory of Open Access Journals (Sweden)

    Walter NICCOLI-FILHO

    2001-06-01

    Full Text Available O laser de dióxido de carbono (CO2, pelas suas propriedades intrínsecas, tem se tornado um instrumento cirúrgico importante; entre estas podemos citar: hemostasia, redução do edema e da dor pós-operatória e esterilização do campo cirúrgico. Seu uso em tecido ósseo para realização de osteotomias ainda é questionável, principalmente devido à possibilidade de iatrogenias causadas pelo aumento da temperatura tecidual, bem como destes efeitos na área paraincisional. O propósito deste estudo foi proporcionar a confecção de um plano piloto visando analisar macroscopicamente os efeitos da radiação laser de CO2 em tíbia de rato a fim de estabelecerem-se parâmetros de segurança quanto a potência. Foram utilizados 12 ratos. Após anestesia, os ossos foram submetidos a radiação com potência de 1, 3 e 5 watts. Os animais foram sacrificados nos tempos imediatamente após, 3, 7 e 14 dias após a irradiação, visando à remoção do osso tratado. Os espécimes obtidos foram observados através de lupa estereoscópica com aumentos de 14, 25 e 40 vezes e fotografados para posterior análise. Os resultados permitiram concluir que a potência de 1 watt proporciona uma melhor qualidade de resposta à reparação e que as potências de 3 e 5 watts provocaram o atraso na cronologia de reparo.The use of CO2 laser in osseous tissue to execute osteotomies is still questionable, mainly due to the increase of temperature in the site of irradiation. The purpose of this research was to carry out a pilot study in order to analyze macroscopically the effect of CO2 laser irradiation on rat tibia and to establish security parameters regarding power. Twelve rats were submitted to irradiation with 1, 3 and 5 watts of power and were examined immediately, 3, 7 and 14 days after irradiation. The results showed better healing when irradiation was carried out with the power of 1 watt; the 3- and 5-watt powers caused delay in the chronology of healing.

  9. Effect of cadmium on the floor of the mouth on rats during lactation Efeito do cádmio no soalho da boca de ratos durante a lactação

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    Lara Cristina Picoli

    2004-06-01

    Full Text Available Cadmium (Cd present in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cd affects placental function, may cross the placental barrier and directly modify fetal development. It is also excreted into milk. The body is particularly susceptible to Cd exposure during perinatal period. The effect on rat oral epithelium (floor of the mouth after continuous exposure to drinking water containing low levels of Cd during lactation was studied. Female rats were supplied with ad libitum drinking water containing 300 mg/l of CdCl2 throughout the whole lactation period. Control animals received a similar volume of water without Cd. Lactating rats (21 day-old were killed by lethal dose of anesthetic. The heads were retrieved, fixed in "alfac" solution (alcohol, acetic acid and formaldehyde for 24 h, serially sectioned in frontal plane, at the level of the first molars. The 6 µm sections were then stained with hematoxylin and eosin. Nuclear epithelium parameters were estimated, as well as cytoplasm and cell volume, nucleus/cytoplasm ratio, numeric and surface densities, and epithelial thickness. Mean body weight was 34.86 g for the control group and 18.56 g for the Cd-treated group. Histologically, the floor of the mouth epithelium was thinner in the treated group, with smaller and more numerous cells. In this experiment, Cd induced epithelial hypotrophy, indicating a direct action in oral mucosa cells, besides retarded development of the pups.O cádmio (Cd do ar, da água e dos alimentos tem o potencial de afetar a saúde das pessoas, principalmente daquelas que vivem em regiões altamente industrializadas. O Cd afeta a função placentária, podendo atravessar a barreira placentária e provocar distúrbios no desenvolvimento fetal. Pode, também, ser excretado pelo leite. O organismo é particularmente susceptível à exposição ao Cd no período perinatal. Foi

  10. Histomorphometric and functional studies of the rat thyroid throughout the estrous cycle Histomorfometria e função tireoidiana no ciclo estral da rata

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    R. Serakides

    2002-02-01

    Full Text Available The influence of the estrous cycle on the thyroid gland was studied. Twenty one five-to-seven-month-old Wistar female rats were divided according to the stage of the estrous cycle in two groups: metaestrus-diestrus and proestrus-estrus. After gross inspection, the thyroids were weighed, sampled, and processed for staining with hematoxilyn-eosin. Seric concentrations of total T4, free T4, total T3, TSH, progesterone, and estradiol were measured. The values of estradiol were significantly higher in the proestrus-estrus stage. However, no significant differences in the plasmatic concentrations of progesterone, free T4, total T4 and TSH throughout the cycle were found. The results of the morphometric study of the thyroid did not indicate any significant differences between the groups. These findings suggest that there is no thyreotrophic effect of estrogen during the estrous cycle in rats.

  11. Efeito da suplementação com pupunha como fonte de vitamina A em dieta: estudo em ratos Effect of supplementation with peach palm as source of vitamin A: study with rats

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    Lucia K.O. Yuyama

    1996-02-01

    Full Text Available Foi estudado o efeito da suplementação com pupunha (Bactris gasipaes H.B.K., polpa cozida e transformada em farinha, como fonte de vitamina A, em dieta regional de Manaus, AM,Brasil, por meio do ensaio biológico com ratos. A metodologia utilizada foi da depleção dos animais em zinco e vitamina A, seguida da repleção com a dieta regional (DR, DR + pupunha, DR + vitamina A e dieta controle (DC. A dieta foi elaborada para famílias com rendimentos inferiores a dois salários-mínimos. Foram utilizadas ratas albinas adultas pós-parto, cada qual com 6 filhotes machos, que receberam ração à base de caseína lavada com EDTA a 1%, sem adição de Zn e vitamina A, por 25 dias, com a finalidade de obtenção de animais recém-desmamados deficientes nestes dois nutrientes. O período de repleção dos animais, recém-desmamados, foi de 30 dias e o delineamento foi inteiramente casualizado com 4 tratamentos de 8 animais cada. A suplementação efetuada seguiu as recomendações do "Committee on Laboratory Animal Diets". Os parâmetros empregados para a avaliação da utilização de vitamina A foram as concentrações de vitamina A no fígado e plasma e o crescimento dos animais. Ao final do experimento, observou-se que os animais que consumiram a ração à base da dieta regional de Manaus, suplementada com pupunha e vitamina A, apresentaram, respectivamente, concentração significativamente maior de vitamina A no fígado, 43,3 ± 6,5 µg/g e 42,0 ± 4,3 µg/g em relação à dieta regional, 5,5 ± 1,1 µg/g (pThe effect of supplementation with peach palm (Bactris gasipaes H.B.K., as an alternative source of vit. A, in the regional diet of Manaus, AM, Brazil, in which the pulp was cooked and transformed into flour, was studied. The biological trial involved rats which were depleted in zinc and vitamin A, followed by repletion using the regional diet (RD, RD plus peach palm and RD plus vitamin A. The parameters used to determine the utilization

  12. Evaluation of peritoneal adhesions formation and tissue response to polypropylene - poli (2-hydroxyethyl methacrylate-(polyHEMA implant on rats' abdominal wall Avaliação da formação de aderências peritoneais e da resposta tecidual ao implante de poli (2-hidroxietil dimetacrilato-(poliHEMA na parede abdominal de ratos

    Directory of Open Access Journals (Sweden)

    Neusa Margarida Paulo

    2010-08-01

    Full Text Available PURPOSE: To verify if the composit poli (2-hydroxyethyl methacrylate-PolyHEMA/polypropylene mesh implanted in the female rat's abdominal wall could be suitable for the prevention of peritoneal adhesions, and for the evaluation of the tecidual response produced by this biomaterial. METHODS: Polypropylene meshes (Group PP, n=20 and polypropylene meshes coated with a layer of poli (2-hydroxyethyl methacrylate-PolyHEMA (Group PH, n=20 were implanted on the abdominal wall of Wistar female rats. Ten animals from each group were submitted to euthanasia at 15 and 30 days of the postoperative period. RESULTS: The animals from the group PP presented visceral adhesions on the mesh surface, which was not observed in the ones from group PH. At the histopathological examination foreign body response was observed in both groups, whilst there was a greater intensity of inflammatory response in group PH on both moments. CONCLUSION: The poli (2-hydroxyethyl methacrylate polyHEMA hydrogel associated to polypropylene mesh reduces visceral adhesion formation in rats, although it may be associated to greater inflammatory reaction.OBJETIVO: Verificar se compósito poli 2-hidroxietil dimetacrilato (PoliHEMA / tela de polipropileno implantado na parede abdominal de ratas seria adequado para prevenção de aderências peritoneais e avaliar a resposta tecidual desencadeada por este biomaterial. MÉTODOS: Foram implantadas telas de polipropileno - Grupo PP (n=20 e telas de polipropileno revestidas por uma camada de poli 2 (hidroxietil dimetacrilato-PolyHEMA - Grupo PH (n=20 na parede abdominal de ratas da linhagem Wistar. Dez animais de cada grupo foram submetidos à eutanásia aos 15 e 30 dias de pós-operatório. RESULTADOS: Os animais do grupo PP apresentaram aderências viscerais na superfície da tela, o que não foi observado nos do grupo PH. Observou-se no exame histopatológico resposta tipo corpo estranho nos dois grupos sendo que no grupo PH houve maior

  13. Effects of oral parenteral nutrition solution on the morphology and mechanical resistance of the small bowel in rats Efeitos da administração oral da nutrição parenteral total na morfologia e resistência mecância do intestino delgado, em ratos

    Directory of Open Access Journals (Sweden)

    José E. Aguilar-Nascimento

    1997-09-01

    Full Text Available The objective of this study was to investigate the effect of an elemental diet (ED on the strength and on the morphology of the small bowel. Male Wistar rats were randomized to two groups to receive during 14 days either standard laboratory rat chow (N=16 or ED (N=16 containing total parenteral nutrition (TPN solution. After this period they were killed and necropsied. The small bowel was measured and weighted with and without the contents. Bursting pressure (BP was taken from the jejunum and ileum and histological sections of these two portions was performed to register the crypt depth (CD, vilus height (VH and wall width (WW. All animals significantly gain weight. The bowel of animals fed with TPN solution had significantly less weight when compared with the controls either with (9.9 ± 1.9g x 7.8 ± 1.5g, pForam submetidos a ração padrão de laboratório 16 ratos e outros 16 receberam nutrição parenteral total (NPT por via oral, durante 14 dias. Os animais de ambos os grupos ganharam peso, sem diferença estatistica. Na necrópsia retirava-se o segmento intestinal a partir do piloro até a valva ileocecal. Não houve diferença no comprimeto do intestino delgado em ambos os grupos. Porém, o intestino dos animais alimentados com NTP apresentaram diminuiçao significante de peso comparado com o grupo controle, independentemente da presença ou não de conteúdo. A resistência intestinal era menor no grupo NTP comparado ao controle, sendo mais significante no íleo. A altura dos vilos, a profundidade das criptas e a extensão da parede eram significantemente menores no íleo no grupo NTP comparado ao controle. Este achado foi observado também no jejuno, mas sem diferença significante. Conclui-se que nos ratos alimentados com NTP o intestino delgado perde massa e torna-se menos resistente, principalmente no íleo,apesar da manutenção do estado nutricional. A perda de massa aparece não apenas na mucosa mas em toda a parede intestinal.

  14. Análise da eficácia do laser de baixa potência no tratamento da dor tendínea em ratos imunossuprimidos Analysis of low-level laser therapy efficacy on tendon pain treatment in immunosuppressed rats

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    Fernanda de Oliveira Busarello

    2011-12-01

    Full Text Available O comprometimento do sistema imune, que pode ser apresentado por indivíduos com doenças crônicas, leva à baixa resposta imunológica. Um dos tratamentos utilizados para lesões agudas em tendões é o laser de baixa potência, contudo há uma lacuna em relação ao seu uso em imunodepressão. O objetivo do presente estudo foi analisar se o laser de baixa potência é eficaz para o tratamento da dor em ratos imunodeprimidos submetidos a trauma tendíneo. Foram utilizados 23 ratos, machos, da linhagem Wistar, divididos aleatoriamente em três grupos: grupo controle, placebo e laser. Os animais foram imunodeprimidos (por administração de Ciclosporina A e submetidos à lesão no tendão calcâneo direito, com impacto de cerca de 0,40 J. Para o tratamento, utilizou-se laser de baixa potência, 670 nm, 30 mW e dose de 2 J/cm², durante 3 dias. A avaliação da dor foi realizada pelo teste de incapacidade funcional e por filamento de Von Frey digital. Os resultados apresentaram valores significativos para o grupo laser, com diminuição de dor funcional e da dor à pressão na superfície plantar e no local lesionado (tendão calcâneo direito. Portanto, concluiu-se que o laser de baixa potência é eficaz para reduzir a dor em ratos imunodeprimidos submetidos a trauma tendíneo.The commitment of immune system, which may be presented by individuals with chronic diseases, leading to a low immune response. One of the treatments used for acute injuries in tendons is the low-power laser, however there is a gap in relation to its use in immunosuppression. The objective of this study was to analyze if low-level laser therapy is effective for the treatment of pain in immunosuppressed rats subjected to trauma tendon. We used 23 male rats of Wistar strain, divided randomly in three groups: control group, placebo and laser. The animals were immunosuppressed (by administration of Cyclosporin A and underwent right Achilles tendon injury, with impact of about

  15. Efeitos da esplenectomia na peritonite causada por lesão traumática do cólon: estudo em ratos Effects of splenectomy on peritonitis produced by a colonic injury: study in rats

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    Luís Sérgio Nassif

    2004-09-01

    Full Text Available OBJETIVO: Estudar o efeito da esplenectomia na infecção intra-abdominal com bactérias da flora enteral, liberadas para a cavidade abdominal através de uma lesão induzida no cólon de ratos Wistar. MÉTODOS: Foram utilizados 64 animais, sendo 20 do Grupo A1 (normais sem sutura da lesão, 22 do Grupo A2 (normais com sutura da lesão e 22 do Grupo B (esplenectomizados e com sutura da lesão. Os animais foram submetidos à laparotomia mediana e a indução da peritonite intra-operatória foi obtida através de lesão do cólon previamente distendido pela introdução de uma sonda naso-gástrica via retal e injeção de 2 ml de soro fisiológico. Foram realizados exames bacteriológicos de lavado abdominal obtido por swab esterelizado e exame microscópico de segmento suturado do cólon de amostras obtidas dos grupos A2 e B com 48hs, 96hs e 12 dias de pós-operatório. Todos os animais foram submetidos a necropsia por ocasião do óbito ou no 12º. dia de pós-operatório quando os sobreviventes foram sacrificados. RESULTADOS: Agentes bacterianos semelhantes foram encontrados nos três grupos: E.coli (100%; Enterococcus faecalis (97%; P. mirabilis (90%; Klebsiela pneumoniae (70%; Citobacter freundi (70% e Enterobacter aglomerans (63%. O exame microscópico revelou menor reação inflamatória no grupo esplenectomizado. A causa da morte na maioria foi peritonite nas primeiras 96hs. Houve alto índice de significância de mortalidade entre os animais do Grupo B (80% em relação ao Grupo A2 (sem mortalidade e em relação ao Grupo A1 (35%. CONCLUSÃO: Houve alto índice de significância de mortalidade em vigência de peritonite nos animais esplenectomizados em relação aos animais que não foram esplenectomizados.BACKGROUND: Study the effects of splenectomy on the intra-abdominal infection by bowel flora, consequent to a colonic injury in Wistar rats. METHODS: We used 64 animals, 20 for Group A1 (normal with colon lesion left open, 22 for Group

  16. Avaliação do pós-condicionamento isquêmico no tratamento da isquemia mesentérica: estudo experimental em ratos Evaluation of ischemic postconditioning effect on mesenteric ischemia treatment: experimental study in rats

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    Carlos Henrique Marques dos Santos

    2009-06-01

    Full Text Available OBJETIVO: Avaliar o efeito do pré e pós-condicionamento isquêmico sobre a lesão tecidual na mucosa intestinal de ratos submetidos ao processo de isquemia e reperfusão mesentérica. MÉTODOS: Foram estudados 30 ratos Wistar, distribuídos em três grupos: grupo A, em que se realizou isquemia (30 minutos e reperfusão (60 minutos mesentérica; grupo B, isquemia e reperfusão mesentérica precedidos pelo pré-condicionamento isquêmico por três ciclos de isquemia e reperfusão com duração de dois minutos cada; grupo C, isquemia e reperfusão mesentérica e, precedendo o início da reperfusão, foi realizado o pós-condicionamento isquêmico por três ciclos de reperfusão e isquemia com duração de dois minutos cada. Ao final, ressecou-se um segmento do intestino delgado para análise histológica. Avaliaram-se os resultados pela classificação de Chiu et al. e procedeu-se ao tratamento estatístico. RESULTADOS: As médias dos graus de lesão tecidual segundo a classificação de Chiu et al. foram: no grupo A, 3,5; grupo B, 1,2; grupo C, 1. A diferença entre o resultado do grupo A com os resultados dos grupos B e C foi considerada estatisticamente significativa (P OBJECTIVE: To assess the preconditioning and postconditioning effect on intestinal mucosal lesions in rats undergone mesenteric ischemia and reperfusion procedure. METHODS: Thirty Wistar rats were studied and divided into three groups: Group A, 10 rats undergone mesenteric ischemia (30 minutes and reperfusion (60 minutes; Group B, 10 rats undergone mesenteric ischemia and reperfusion preceded by ischemic preconditioning for three cycles of ischemia and reperfusion for two minutes each; Group C, 10 rats undergone mesenteric ischemia and reperfusion and, preceding the beginning of reperfusion, ischemic postconditioning was performed for three cycles of reperfusion and ischemia for two minutes each. Then, a segment of small intestine was resected for histological analysis. We

  17. Facial symmetry evaluation after experimentally displaced condylar process fracture in methotrexate treated rats Avaliação da simetria facial após fratura experimental com desvio do processo condilar em ratos tratados com metotrexato

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    Samantha Cristine Santos Xisto Braga Cavalcanti

    2012-03-01

    Full Text Available PURPOSE: To investigate the facial symmetry of high and low dose methotrexate (MTX treated rats submitted to experimentally displaced mandibular condyle fracture through the recording of cephalometric measurements. METHODS: One hundred male Wistar rats underwent surgery using an experimental model of right condylar fracture. Animals were divided into four groups: A - saline solution (1mL/week; B - dexamethasone (DEX (0,15mg/Kg; C - MTX low dose (3 mg/Kg/week; D - MTX high dose (30 mg/Kg. Animals were sacrificed at 1, 7, 15, 30 and 90 days postoperatively (n=5. Body weight was recorded. Specimens were submitted to axial radiographic incidence, and cephalometric mensurations were made using a computer system. Linear measurements of skull and mandible, as well as angular measurements of mandibular deviation were taken. Data were subjected to statistical analyses among the groups, periods of sacrifice and between the sides in each group (α=0.05. RESULTS: Animals regained body weight over time, except in group D. There was reduction in the mandibular length and also changes in the maxilla as well as progressive deviation in the mandible in relation to the skull basis in group D. CONCLUSION: Treatment with high dose methotrexate had deleterious effect on facial symmetry of rats submitted to experimentally displaced condylar process fracture.OBJETIVO: Avaliar a simetria facial de ratos tratados com metotrexato (MTX, em dose alta e baixa, submetidos à fratura experimental do processo condilar com desvio por meio de mensurações cefalométricas. MÉTODOS: Cem ratos Wistar machos foram submetidos a procedimento cirúrgico utilizando modelo experimental de fratura de côndilo do lado direito. Os animais foram distribuídos em quatro grupos: A - soro fisiológico (1mL/semana; B - dexametasona (DEX (0,15mg/Kg; C - MTX baixa dose (3mg/Kg/semana; D - MTX alta dose (30mg/Kg. Os períodos de sacrifício foram de 1, 7, 15, 30 e 90 dias de pós-operatório (n=5

  18. Características ultraestruturais do segmento abdominal da aorta de rato albino - DOI: 10.4025/actascibiolsci.v29i4.825 Mural features of the abdominal aortic segment of albino rat - DOI: 10.4025/actascibiolsci.v29i4.825

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    Karina Simões

    2008-02-01

    Full Text Available O objetivo da presente pesquisa foi investigar as peculiaridades ultraestruturais da parede da aorta de rato. Foram utilizados sete ratos albinos, adultos jovens, dos quais foram coletados fragmentos da aorta abdominal infra-renal. Após a coleta, os segmentos vasculares foram fixados e encaminhados para a rotina de microscopia eletrônica de transmissão e varredura. As lamelas elásticas aparecem interpostas às fibras musculares lisas, sendo essa disposição principalmente notada na túnica média da parede vascular. Entre as fibras musculares lisas e as lamelas elásticas, observa-se um inter-relacionamento aparentemente estreito, feito por conexão e ancoramento entre ambos os elementos murais por meio de lamelas de colágeno. A túnica íntima da aorta abdominal do rato mostra algumas peculiaridades ultraestruturais marcantes, tais como a interrupção, em certos locais da parede, de continuidade da lâmina elástica interna, interrupção acompanhada por poros endoteliais, de certa extensão, suprajacentes à falha na estrutura elástica intimal. Este padrão de constituição mural, com destaque aos ancoramentos elástico-musculares, via o colágeno, parece garantir propriedades fundamentais da parede vascular, concernentes à hemodinâmica, tal como o cisalhamento, normalmente notado entre os estratos superpostos da parede vascular, bem como a contratilidade e a visco-elasticidade da parede arterial.The objective of the present research was to investigate the ultrastructural peculiarities of the aortic wall of the rat. Seven young adult rats were used, from which fragments of the infrarenal abdominal aorta were collected. After collection, the vascular segments were fixed and sent for analysis by scanning electron microscope. The elastic lamellae appear interposed with smooth muscular fibers; this pattern was verified mainly at the medial layer structure. Among the mural elements a well defined interrelationship was established through

  19. Effect of conjugated equine estrogens and tamoxifen administration on thyroid gland histomorphology of the rat Os efeitos dos estrogênios conjugados equinos e do tamoxifeno na histomorfologia da glândula tireóide de ratas

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    Luiz Felipe Bittencourt de Araujo

    2006-08-01

    Full Text Available OBJECTIVE: The aim of this study was to evaluate the action of the conjugated equine estrogens and tamoxifen on the morphology of thyroid gland in ovariectomized (OVx rats. METHODS: Conjugated equine estrogens (CEE, clinically used as estrogen therapy, is a complex formulation containing multiple estrogens that decrease menopausal symptoms. Thirty ovariectomized rats were randomly divided into 3 treatment groups: GI, vehicle (propylene glycol; GII, CEE 200 µg/kg per day; and GIII, tamoxifen 1 mg/kg per day. Another group of 10 rats with intact ovaries (GIV was included, treated with the vehicle, and sacrificed during estrous. All animals were treated by gavage for 50 days, after which they were sacrificed. Blood samples were collected, and the thyroid was removed for morphological analysis and PCNA evaluation through immunohistochemical study. RESULTS: The thyroid follicular cell height was increased in animals treated with CEE (14.90 ± 0.20 µm, with TAM (14.90 ± 0.10 µm, and in rats with intact ovaries (15.10 ± 0.50 µm in comparison to that of the vehicle group (9.90 ± 0.20 µm (P OBJETIVO: Avaliar a ação dos estrogênios conjugados eqüinos e do tamoxifeno na histomorfologia da tireóide de ratas. MÉTODO: Estrogênios conjugados eqüinos são ministrados clinicamente como terapia estrogênica e contêm formulação complexa com muitos tipos de estrogênios que diminuem os sintomas da pós-menopausa. Trinta ratas adultas ooforectomizadas foram divididas aleatoriamente em três grupos: GI - veículo (propilenoglicol; GII - ECE 200 µg/Kg por dia; e GIII - TAM 1 mg/Kg por dia. Acrescentou-se ainda um grupo de 10 animais com os ovários intactos e tratados com veículo (GIV. Todos os animais foram tratados por gavagem durante 50 dias consecutivos, ao final foram coletadas amostras do sangue e a tireóide removida e processada para análise morfológica e imunohistoquímico para avaliar o PCNA. RESULTADOS: A maior altura das c

  20. Tensile strength study of the abdominal wall following laparotomy synthesis using three types of surgical wires in Wistar rats Estudo da resistência tênsil da parede abdominal após síntese de laparotomia usando três tipos de fios cirúrgicos em ratos Wistar

    Directory of Open Access Journals (Sweden)

    Lucas Félix Rossi

    2008-02-01

    Full Text Available PURPOSE: To study the tensile strength of the abdominal wall following laparotomy synthesis utilizing three types of surgical wires. METHODS: Thirty Wistar rats were randomized into three groups of ten rats each. Each group underwent a 3cm-laparotomy which was closed with 3-0 polyglactin 910, polyglecrapone and catgut wires. After 63 days, euthanasia was performed and part of the abdominal wall was removed with which a strip was produced measuring 2.0 cm in length by 6.0 cm in width comprising the abdominal muscles with the implanted mesh. The sample was fixed in a mechanical test machine in which constant force was applied contrary to the tissue strips. Maximum force was considered, expressed in Newton, until full rupture of the tissue occurred. The non-parametrical Kruskal - Wallis test was used for the statistical analysis, admitting pOBJETIVO: Estudar a resistência tênsil da parede abdominal após síntese de laparotomia utilizando três tipos de fios cirúrgicos. MÉTODOS: Trinta ratos da linhagem Wistar randomizados em três grupos de dez exemplares cada um. Em cada grupo fez-se uma laparotomia de dois centímetros que foi fechada com fios 3-0 de poliglactina 910, poliglecaprone e categute. Após 63 dias, foi feita a eutanásia e retirou-se uma área da parede abdominal com a qual fez-se uma tira medindo 2,0 cm de comprimento por 6,0 cm de largura englobando os músculos abdominais com a tela implantada. A amostra foi fixada em máquina de ensaios mecânicos na qual se aplicou força constante contrária às tiras de tecido. Foi considerada a força máxima expressa em Newton até ocorrer a ruptura total da amostra. Para a análise estatística, utilizou-se teste não paramétrico de Kruskal - Wallis admitindo-se p<0,05. RESULTADOS: A média de resistência do grupo categute foi ligeiramente menor (33.50 N ao da poliglactina (34.23 N, sendo essa diferença não estatisticamente significativa (p=0,733. O grupo poliglecaprone foi o que

  1. Influence of S(+-ketamine analgesia in renal intraoperative ischemia: histological study in rats Influência da analgesia com S(+-cetamina em isquemia renal intra-operatória: estudo histológico em ratos

    Directory of Open Access Journals (Sweden)

    Eloy Rusafa Neto

    2006-08-01

    Full Text Available PURPOSE: To study in rats the effect of S(+­ketamine on the renal histology after intraoperative hemorrhage. METHODS: Twenty male Wistar rats, anesthetized with sodium pentobarbital, were randomly divided in 2 groups: G1 - control (n=l0 and G2 - S(+-ketamine (n=10, both submitted to arterial hemorrhage of 30% of volemia in 3 moments (10% each 10 min 60 min after anesthesia. G2 received S(+-ketamine, 15 mg. kg-1, i.m., 5 min after anesthesia and 55 min before the 1st hemorrhage moment (Ml. Medium arterial pressure (MAP, rectal temperature (T and heart rate were monitored. The animals were sacrificed in M4, 30 min after the 3rd hemorrhage moment (M3 and the kidneys and blood collected from hemorrhage were utilized for histological study and hematocrit (Ht determination. RESULTS: There were significant reduction of MAP, T, and Ht. The histological study verified G1 = G2 for tubular dilation, congestion, and necrosis. The total score addition were significant1y different and G2 > G 1. CONCLUSION: Hemorrhage and hypotension determined changes in kidney histology. The rise in catecholamine blood concentration probably was the cause of S(+-ketamine-induced higher score of histological changes.OBJETIVO: Investigar, em ratos, o efeito da S(+cetamina na histologia renal após hemorragia intra-operatória. MÉTODOS: Vinte ratos Wistar machos, anestesiados com pentobarbital sódico, foram divididos, aleatoriamente, em 2 grupos: G1 - controle (n=10 e G2 - S(+cetamina (n=10, submetidos a hemorragia de 30% da volemia em 3 momentos (10% a cada 10 min 60 min após anestesia. G2 recebeu S(+cetamina, 15 mg. kg-1, i.m., 5 min após anestesia e 55 min antes do 1.º momento de hemorragia (M1. Foram monitorizadas a pressão arterial média (PAM, temperatura retal (T e freqüência cardíaca. Os animais foram sacrificados (M4 30 min após o 3.º momento de hemorragia (M3. Os rins e o sangue das hemorragias foram utilizados para estudo histológico e do hemat

  2. Chondroprotectant therapy in rats with degenerative joint disease experimentally transected cranial cruciateReprodução experimental da doença articular degenerativa, pelo método cirúrgico associado à terapia condroprotetora, em ratos

    Directory of Open Access Journals (Sweden)

    Marcos Marini Melo

    2013-06-01

    Full Text Available Osteoarthritis (OA is the most common joint disease in both humans and animals, and it results in movement restriction and pain at the affected area. This disorder affects more than 25% of people over 60 years of age, and it is considered universal for 70-year-old people. OA is estimated to affect over 20% of the canine population from the United States of America. The present study aimed to evaluate the treatment of rats that were surgically induced to OA using two different drug therapies, one with pentosan polysulfate, one with betamethasone, and one with chondroitin sulfate/ glucosamine. In order to produce joint disease, the cranial cruciate ligament was surgically transected. Animals were kept and treated for eight days after surgery and were assessed via both radiographies and tomographies taken before surgery and eight weeks later. All animals were euthanized having both macroscopic and microscopic analysis performed to evaluate the disorder progression and therapeutic action. Macroscopic analysis showed lesion in the knees subjected to OA induction. The untreated animals presented major lesions whereas the treated ones presented mild to moderate lesions. In conclusion, pentosan polysulfate is recommendable for the treatment of iatrogenic joint lesions in rats since the other treatments showed no significant difference. A osteoartrite (OA é a doença articular mais comum em humanos e animais, o que ocasiona restrição de movimentos e dor, na região acometida. Tal enfermidade afeta mais de 25% dos humanos acima de 60 anos e, aos 70 anos, ela é considerada universal. Estima-se que nos Estados Unidos da América, 20% ou mais da população canina é acometida pela OA. O presente estudo tem como finalidade avaliar a terapia medicamentosa (polisulfato de pentosano, betametasona e sulfato de condroitina/glucosamina, em um modelo experimental de OA cirurgicamente induzido, em ratos. A doença articular foi promovida pela transecção cir

  3. Analysis of protein profiles in diabetic rat blood plasma that induced by alloxan

    Science.gov (United States)

    Hidayati, Dewi; Abdulgani, Nurlita; Setiyawan, Hengki; Trisnawati, Indah; Ashuri, Nova Maulidina; Sa'adah, Noor Nailis

    2017-06-01

    Proteomics is the study to identify the proteins involved in physiological metabolic pathway. The protein profiles of blood plasma from alloxan-induced diabetic rats has investigated using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Data were analyzed descriptively based on variations of the type and intensity of the protein. There were identified the similarity of protein variant between diabetic and control rats included ankyrin (200kDa), IgG (150kDa), nephrin (136 kDa), IDE (112 kDA), albumin (66 kDa), prealbumin (55 kDA), CICP (43 kDa), ApoA-V (39 kDa), GAPDH (35 kDa), C-RP (27,1 kDa), leptin (16 kDa) and apelin (13 kDa). However, the apelin profile at diabetic rats shows the higher intensity than control.

  4. Índices bióticos para avaliação da qualidade ambiental em trechos do rio Correntoso, Pantanal do Negro, Estado do Mato Grosso do Sul, Brasil = Biotic indexes for the evaluation of environmental quality in stretches of the Correntoso river, Pantanal do Negro, Mato Grosso do Sul State, Brazil

    Directory of Open Access Journals (Sweden)

    Fábio Henrique da Silva

    2011-07-01

    Full Text Available Este trabalho teve como objetivo pesquisar a estrutura da comunidade de insetos aquaticos, utilizando esta comunidade como indicadora da qualidade ambiental de um trecho do rio Correntoso, Pantanal do Negro, Mato Grosso do Sul, Brasil. Foram realizadas seis campanhas de coleta em diferentes periodos sazonais: vazante, seca e cheia. Os organismos foram coletados utilizando rede D com malha de 300 ƒÝm eesforco amostral de cinco lances de rede nas raizes dos bancos de macrofitas. Foram comparados tres ambientes (aberto, intermediario e fechado, por juncao dos dados obtidos de seis sitios de coleta. Foi analisada a riqueza, abundancia absoluta e relativa das familias de insetos amostrados e a avaliacao da qualidade da agua usando o indiceBMWP, indice BMWP-ASPT, indice IBF e indice de diversidade de Shannon-Wienner, com log2. Foram registradas 60 familias pertencentes a 12 ordens da Classe Insecta, totalizando 19.773 individuos coletados. Entre os indices aplicados, o indice BMWP foi o que melhor representou as condicoes do ambiente estudado.This work aimed to research the aquatic insect community structure using this community as a bioindicator of the environmental quality in a stretch of the Correntoso river, in Pantanal do Negro, Mato Grosso do Sul, Brazil. Six collections were taken in different seasonal periods; ebb, dry and wet. The organisms were collected using a 300 ƒÝm mesh D net sweeping five times through the roots of macrophyte banks at each sample. Three environments were compared (open, intermediary, closed by adding the information from six collection sites. Family richness, absolute and relative abundance ofinsect samples and an evaluation of water quality were analyzed by using the BMWP index, BMWP-ASPT index, IBF index and Shannon diversity index, with log2. A total of 60 families from 12 orders of Insecta Class were recorded, totaling 19,773 individuals. Among the indexes applied, the BMWP index was the one that best

  5. Alterações histoquímicas das glicosaminoglicanas na cérvice uterina no final da prenhez da rata albina após ministração local de hialuronidase Histochemical changes of the glycosaminoglycans in the uterine cervix of pregnant rats after local injection of hyaluronidase

    Directory of Open Access Journals (Sweden)

    Viviane Almeida de Alcântara Lopes

    2008-07-01

    Full Text Available OBJETIVO: estudar as alterações histoquímicas relacionadas às glicosaminoglicanas da cérvice uterina da rata albina, após ministração local de hialuronidase no final da prenhez. MÉTODOS: dez ratas com teste de prenhez positivo foram distribuídas aleatoriamente em dois grupos, numericamente iguais. O Grupo Controle (Gc foi constituído pelas ratas que receberam 1 mL de água destilada, dose única, no 18º dia da prenhez, sob anestesia, ministrado na cérvice uterina. O Grupo Experimental (Gex constou de ratas que receberam, sob as mesmas condições do Gc, 0,02 mL de hialuronidase, diluído em 0,98 mL de água destilada (total de 1 mL. No 20º dia de prenhez, as ratas foram novamente anestesiadas e submetidas à dissecção, preparando-se a cérvice uterina para estudo histoquímico com coloração de alcian blue e seus bloqueios (pH=0,5, pH=2,5, metilação e saponificação. RESULTADOS: verificou-se na lâmina própria no Gc, reação fortemente positiva (+3 e, no Gex, reação negativa, na coloração de alcian blue no pH=0,5. Em pH=2,5 a coloração também se apresentou fortemente positiva (+4 no Gc e fracamente positiva (+1 no Gex. Após metilação, tanto o Gc quanto o Gex mostraram reação negativa após coloração de alcian blue no pH=2,5. Com a reação de metilação seguida de saponificação e na digestão enzimática em lâmina, a coloração da lâmina própria se mostrou negativa em ambos os grupos. CONCLUSÕES: há uma nítida predominância de glicosaminoglicanas sulfatadas no Gc em relação ao Gex e uma tênue quantidade de glicosaminoglicanas carboxiladas identificadas no Gex. As modificações evidenciadas na matriz extracelular sugerem que a hialuronidase injetada localmente na cérvix uterina promoveu alterações bioquímicas compatíveis com maturação cervical.PURPOSE: to study the histochemical changes related to the uterine cervix glycosaminoglycan of the albino female rat, after local ministration of

  6. Effect of gala apples (Malus domestica Borkh on lipidemia of hyperlipidemic rats Efeito da maçã gala (Malus domestica Borkh na lipidemia de ratos hipercolesterolêmicos

    Directory of Open Access Journals (Sweden)

    Jocelem Mastrodi Salgado

    2008-06-01

    Full Text Available A healthy life style and a balanced diet, associated with a high fruit and vegetable intake, are linked to good health and the prevention of diseases. Apples contain bioactive compounds that help in the prevention and control of hyperlipidemia. One of the Public Health concerns in Brazil is to reduce cardiovascular diseases. Therefore, the objective of this work was to analyze the chemical composition of Gala apples and to study the effect of their consumption on weight gain, food intake, serum levels of total cholesterol, HDL-C, LDL-C, triglycerides, hepatic cholesterol and fecal cholesterol in male albino Wistar rats fed a hypercholesterolemic diet. Six animals were utilized for each treatment (control, 5, 15 and 25% apple diet, during 30 and 60 days. This study showed that one apple (200 g can provide 14.5% of recommended total fiber and 55% of recommended vitamin C, besides supplying considerable quantities of phenolic compounds (0.38 g.100 g-1 and tannins (0.16 g.100 g-1. All animals showed a non-significant reduction in their weight gain and food intake with an increase in the concentration of apple in the diets. At the end of 30 days, all of the diets provided a significant reduction in the levels of triglycerides compared to the control group. The 15 and 25% apple diets showed significant reductions in the serum levels of total cholesterol and LDL-C and an increase in the level of fecal cholesterol in relation to the control group. The 25% apple diet provided a significant reduction in the hepatic cholesterol levels compared to the control group. After 60 days, the serum levels of total cholesterol, LDL-C, HDL-C and triglycerides in rats fed with 5, 15 and 25% apple diets were similar to the control group. This probably happened due to a revertion of the process. These results show the importance of Gala apples in the control of hyperlipidemia in rats. A diet rich in vegetables and fruits, including apples, associated to a healthy life

  7. Quality evaluation of Argentina onion irradiated, transported and stored; Avaliacao da qualidade da cebola argentina irradiada, transportada e armazenada no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Walder, J M.M.; Domarco, R E; Spoto, M H.F.; Blumer, L [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil); Curzio, O A; Croci, C A [Universidad Nacional del Sur, Bahia Blanca (Argentina). Lab. de Radioisotopos

    1996-12-31

    Onion bulbs of the Valenciana Sintetica 14 variety from Argentina were irradiated with a dose of 60 Gy gamma rays at 30-40 days after harvest. Treated and control lots (40 bags of 20 Kg each) were send by road to Piracicaba, SP, Brazil, and stored up to 180 days in a warehouse at 20-28{sup 0} C and relative humidity ranging from 50 to 100%. Monthly quality control was done by physical and sensorial analysis. Radiation treatment reduced the weight loss of the bulbs and increased the percentage of marketable bulbs without affecting its sensorial properties. (author). 4 refs, 8 tabs.

  8. Ribeira do Iguape basin water quality assessment for drinking water supply; Avaliacao da qualidade da agua na bacia hidrografica do Ribeira de Iguape com vistas ao abastecimento publico

    Energy Technology Data Exchange (ETDEWEB)

    Cotrim, Marycel Elena Barboza

    2006-07-01

    Ribeira do Iguape Basin, located in the Southeast region of Sao Paulo state, is the largest remaining area of Mata Atlantica which biodiversity as rich as Amazon forest , where the readiness of water versus demand is extremely positive. With sparse population density and economy almost dependent on banana agriculture, the region is still well preserved. To water supply SABESP (Sao Paulo State Basic Sanitation Company). Ribeira do Iguape Businesses Unit - RR, uses different types of water supplies. In the present work, in order to ascertain water quality for human consumption, major and minor elements were evaluated in various types of water supply (surface and groundwater's as well as the drinking water supplied). Forty three producing systems were monitored: 18 points of surface waters and treated distributed water, 10 points of groundwater and 15 points of surface water in preserved areas, analyzing 30 elements. Bottom sediments (fraction < 63 {mu}m) were also evaluated. The sampling period covered dry and wet seasons from March 2002 to February 2003. The descriptive analysis showed that Al, Fe and Mn, exceeding CONAMA 357 quality guideline. A comparison of the elemental concentrations with the Brazilian Drinking Water Legislation (Portaria 518/04) showed that with the exception of some violations, the levels of all the elements investigated were below the Brazilian Legislation maximum allowed concentrations. This study examined the relationship between the type of water supply and the quality of water used, showed different characteristics on Ca, Fe, Mn concentration. In bottom sediments (fraction <63 {mu}m), Al, Fe and Mg largest concentrations were found. Pb and Zn presents concentrations up to 142,0 {mu}g.g{sup -1} and 172 {mu}g.g{sup -1}, respectively. Data revealed that trace elements concentration in the sediment were below PEL (Probable Effect Level - probable level of adverse effect to the biological community), exception for Pb in Sete Barras and Eldorado. (author)

  9. Density evaluation by computerized tomography in plain soils over different manipulation systems; Avaliacao da densidade pelo metodo da tomografia computadorizada de um planossolo sob diferentes sistemas de manejo

    Energy Technology Data Exchange (ETDEWEB)

    Pedrotti, Alceu [Lavras Univ., MG (Brazil). Dept. de Ciencia do Solo; Pauletto, Eloy Antonio [Pelotas Univ., RS (Brazil). Dept. de Solos; Crestana, Silvio [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Sao Carlos, SP (Brazil). Centro Nacional de Pesquisa e Desenvolvimento de Instrumentacao Agropecuaria (CNPDIA)

    1996-08-01

    The objective of this paper is the evaluation of a plain soil density in different culture systems determined by X ray computerized tomography.It was observed a larger variation in densities in soils profiles analysed. The identification of layers is better utilising computerized tomography than others technic 1 fig.

  10. Assessing the quality of energy supplied by small renewable energy systems; Avaliacao da qualidade da energia fornecida por sistemas renovaveis isolados de pequeno porte

    Energy Technology Data Exchange (ETDEWEB)

    Galhardo, Marcos Andre Barros; Pinho, Joao Tavares [Universidade Federal do Para (UFPA), Belem, PA (Brazil). Grupo de Estudos e Desenvolvimento de Alternativas Energeticas], e-mail: galhardo@ufpa.br, E-mail: jtpinho@ufpa.br

    2004-07-01

    This paper makes an analysis of the power quality supplied by small renewable energy systems with energy storage in a battery bank and its DC/AC conversion by an inverter. It is shown that the power quality supplied by stand-alone renewable systems depends on the used inverter, specially of its voltage waveform, the load supplied and the voltage level in the battery bank, which has a direct influence on the value of the rms output voltage of the inverter. For these analysis, measurements are presented for typical loads used in these systems as illumination, TV, refrigerator, etc., and combinations of these loads, supplied by different waveforms. (author)

  11. Study and evaluation of radiometry in photo therapeutic treatment of the neonatal hyperbilirubinaemia; Estudo e avaliacao da radiometria no tratamento fototerapico da hiperbilirrubinemia neonatal

    Energy Technology Data Exchange (ETDEWEB)

    Caly, Jose Pucci

    2009-07-01

    Phototherapy is a procedure established more than 50 years ago in the treatment of the newborn jaundice. However there is no a standard method to quantify the photo therapeutic dose in published clinical studies, hindering the comparison of previous studies on photo therapeutic effectiveness, as well as the establishment of safe and predictable doses. The photo therapeutic dose depends, among other factors, on the effective mean irradiance produced by the photo therapeutic unit. There are no standard procedures, however, neither to quantify the effective irradiance, nor to estimate the mean effective irradiance. As a consequence, large measurement variations in a same photo therapeutic unit are observed using different commercially available radiometers, as a consequence of the vast diversity of spectral responsivities of the instruments. An objective of this work was to adapt and to apply the bases of the wideband ultraviolet radiometry to quantify the available irradiance from photo therapeutic units, establishing procedures that allow us to compare measured irradiances from different sources, using radiometers presenting different spectral responsivities. Another objective was to characterize samples of photo therapeutic units commonly used, focusing the problem of the estimation of the effective mean irradiance from photo therapeutic units, proposing a method to estimate of the effective irradiance from focused sources. The experimental results allow us to conclude that it is not only necessary to standardize the photo therapeutic radiometry, but also the method of estimation of the effective mean irradiance. (author)

  12. Evaluation of X ray attenuation by means of radiographic images; Avaliacao da atenuacao da radiacao X por meio de imagens radiograficas

    Energy Technology Data Exchange (ETDEWEB)

    Barros, Frieda Saicla, E-mail: saicla@utfpr.edu.b [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Paredes, Ramon S.C., E-mail: ramon@ufpr.b [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil); Godoi, Walmor C., E-mail: walmor.godoi@gmail.co [Faculdade de Tecnologia Camoes (FATEC), Curitiba, PR (Brazil); Souza, Gabriel Pinto de [Universidade Estadual Paulista (UNESP), SP (Brazil)

    2011-07-01

    This paper's main goal is to adopt a qualitative methodology to evaluate the attenuation of x-radiation through X-ray images in polymeric materials plus residual lead. To determinate the images it was initially used an experimental setup at the Laboratory for Materials Diagnostics LACTEC. These results correspond to a more qualitative analysis, even with quantitative answers. Through analysis of radiographic images we can measure the intensity of radiation that goes through the plate, making possible to establish a relationship between the attenuation coefficient and the thickness of the material. (author)

  13. Evaluation of the morphological alteration of the root surface radiated with a diode laser; Avaliacao da alteracao morfologica da superficie cimentaria irradiada com laser de diodo

    Energy Technology Data Exchange (ETDEWEB)

    Gulin, Mauricio

    2003-07-01

    The diode laser has been studied for periodontal therapy, as much for removal of calculus as for microbial reduction of periodontal pockets, as well as the visible analgesic effects and biomodulation capacity. For this reason the purpose of this study was to evaluate the morphological alteration of the root surface after radiation with the diode laser, 808 nm through analysis by scanning electron microscopy (SEM). Besides this, to verify the temperature variations caused during the radiation, a thermometer put into the dentinal wall of the root canal was used. In all, 18 teeth were used, 15 of which for the SEM study, and the other 3 were used to temperature variation analysis. The 25 samples were scraped on the root surface and planed with manual instruments. The other 5 were not subjected to any type of treatment. This, 6 groups of 5 samples each were formed. Control Group C whose samples had not received any treatment; Control Group C 1 was only scraped and polished conventionally with Hu-Friedy Gracey curettes 5 and 6; the other samples groups L1, L2, L3, L4 were radiated by diode laser using parameters of power 1,0 W; 1,2 W; 1,4 W; and 1,6 W respectively, 2 times for 10 seconds with 20 seconds intervals between each radiation in continuous mode. The results with relation to the increase of temperature in the interior of the root canal demonstrated that there was an increase of more than 5 degree Celsius. The results of the scanning electron microscope analysis of Control Group C demonstrated great irregularity and ridges on the root surface, with the presence of a dentine layer. Control Group C1 presented a similar aspect to Group L 1's, smoother and more homogeneous surface. Groups L2, L3, and L4 presented scratches alternating with smoother areas showing that fiber contacted the surface of the sample. The results reconfirmed the necessity of further studies using diode laser, with a beam of light emitted in an interrupted mode to improve the control of the increase of temperature during radiation, and probably followed by a new scraping and root polishing with conventional instruments in an attempt to obtain a smoother and more homogeneous root surface. (author)

  14. Technique applied in the evaluation of the UFCC reactor at Refinaria Alberto Pasqualini (REFAP), PETROBRAS; Tecnicas utilizadas na avaliacao do reator da UFCC da REFAP

    Energy Technology Data Exchange (ETDEWEB)

    Cascaes, Ronesio da Silva [PETROBRAS (Brazil) Refinaria Alberto Pasqualini

    1994-01-01

    Main problems found in the maintenance of the Fluidized Catalytic Cracking (FCC) unity, searches of metal alteration properties, metallographic evaluations, failures and micro-structures alterations are presented. Micrographic analysis of the structural samples, the methodology to detect the failures and physical and chemical essays utilized in the inspection are also reported 13 refs., 64 figs., 1 tab.

  15. Quality assessment of radiopharmaceuticals in nuclear medicine services at Northeast states, Brazil; Avaliacao da qualidade de radiofarmacos em servicos de medicina nuclear de estados da regiao nordeste

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Wellington Gomes de

    2012-07-01

    The radiopharmaceuticals are used in the field nuclear medicine services (NMS) as tracer in the diagnoses and treatment of many diseases. Radiopharmaceuticals used in nuclear medicine and usually have a minimum of pharmacological effect. The procedures for labelling Radiopharmaceuticals should be observed in order to minimize risks to patients, employees and individuals from the public, and to be administered in humans, must be sterile and free of pyrogens and possess elements all measures of quality controls required a conventional drug. The 'Agencia Nacional de Vigilancia Sanitaria (ANVISA)' in its 'Resolucao de Diretoria Colegiada' (RDC) No. 38 of June 4{sup th} 2008, decided that the NMS must perform quality control in the generators eluate and radiopharmaceuticals according to recommendations of manufacturers and scientific evidence accepted by ANVISA. Thus, this study proposes to evaluate the quality of the generator {sup 99M}o-{sup 99m}Tc eluate and radiopharmaceuticals labeled with {sup 99m}Tc used in most NMS of some states in the Northeast, in relation to radionuclide, chemical, radiochemical purity and pH and promote the inclusion of procedure for quality control of radiopharmaceuticals in routine NMS. The results show that 90% radionuclidic purity, 98.2% purity chemical and radiochemical purity of 46% and 100% of the eluates are in agreement with international pharmacopoeias; already radiopharmaceuticals showed 82.6% purity and all radiochemical pH values are also in accordance with international pharmacopoeias. Even with so many positive results, staff the majority of MNS was not able to perform the quality control of the eluates and radiopharmaceuticals. Showing the importance of implementing of quality control programs of the eluates and radiopharmaceuticals in nuclear medicine. (author)

  16. Synthesis and evaluation of phytotoxic activity of {alpha}-Santonin derivatives; Sintese e avaliacao da atividade fitotoxica de derivados da {alpha}-Santonina

    Energy Technology Data Exchange (ETDEWEB)

    Alvarenga, Elson S.; Barbosa, Luiz C.A.; Saliba, William A.; Arantes, Francisco F.P.; Demuner, Antonio J. [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Quimica]. E-mail: elson@ufv.br; Silva, Antonio A. [Universidade Federal de Vicosa (UFV), MG (Brazil). Dept. de Fitotecnia

    2009-07-01

    Mixtures of {alpha}-Santonin and various solvents were irradiated by either high or low pressure mercury lamps. The photochemical reactions afforded lumisantonin (11) (76% in acetonitrile), (3 S,3a S,9{beta}S)-3,6,6-trimethyl-3,3a,4,5-tetrahydronafto[1,2-b]furan-2,7({eta}6,9{beta}{eta}) dione (12) (100% in acetonitrile), 10{alpha}-acetoxy-3-oxo-1,7{alpha}H{eta},6,11{alpha}a{eta}-guaia-4-en-6,12-oli= de (8) (26% in acetic acid), 10{alpha}-hydroxy-3-oxo-1,7{alpha}a{eta},6,11{alpha}{eta}-guaia-4-en-6,12-olid= e (10) (32%) and (E)-3-((3 S,3a S,7{alpha}S)-3-methyl-2-oxo-6-(propan-2-ylidene)hexahydrobenzofuran- 7 - (7{alpha}{eta})-ylidene)propanoic acid (9) (44%) (in water/ acetic acid 1:1, v/v). Lactone 12 was also prepared by irradiation of lumisantonin in diethyl ether. Lactones 8 and 10 were converted, respectively, into the 10 {alpha}-acetoxy-3{alpha}-hydroxy-1,7{alpha}H,6,11{alpha}H-guaia-4-en-6,12-olid= e (13) (87%) and 3a,10a-dihydroxy-1,7{alpha}H,6,11{alpha}H-guaia-4-en-6,12-olide (14) (75%) by sodium borohydride reduction. The effects of the compounds on the development of radicle of Sorghum bicolor and Cucumis sativus were evaluated. (author)

  17. Development of test objects for image quality evaluation of digital mammography; Desenvolvimento de objetos de teste para avaliacao da qualidade da imagem em mamografia digital

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Vitor Nascimento de Carvalho

    2013-07-01

    Mammography is the image exam called 'gold standard' for early detection of breast cancer. 111 Brazil, more than eight million mammograms are carried out per year. With the advancement of technology, the digital systems CR and DR for this diagnostic modality have been increasingly implemented, replacing the conventional screen-film system, which brought environmental problems, like the disposal of chemical waste, and is also responsible for the rejection of radiographic films with processing artifacts. Digital systems, besides not experiencing the problem of environmental pollution, are still capable of image processing, allowing a much lower rejection rate when compared to the conventional system. Moreover, the determination of an accurate diagnosis is highly dependent on the image quality of the examination. To ensure the reliability of the images produced by these systems, it is necessary to evaluate them on a regular basis. Unfortunately, there is no regulation in Brazil about the Quality Assurance of these systems. The aim of this study was to develop a set of test objects that allow the evaluation of some parameters of image quality of these systems, such as field image uniformity, the linearity between the air Kerma incident on detector and the mean pixel value (MPV) of the image, the spatial resolution of the system through the modulation transfer function (MTF) and also to suggest an object to be applied in the evaluation of contrast-to-noise ratio (CNR) and signal-difference-to-noise ratio (SDNR). In order to test the objects. 10 mammography centers were evaluated, seven with CR systems and three with the DR systems. To evaluate the linearity, besides the test objects high sensitivity dosimeters were necessary to be used, namely LiF:Mg,Cu,P TL dosimeters. The use of these dosimeters was recommended in order to minimize the time required to perform the tests and to decrease the number of exposures needed. For evaluation of digital images in DICOM format, the software {sup O}BJ{sub I}QJeduced{sup ,} version 3.0, was used. It was developed by the team of Prof.. Dr. Nicholas Marshall at the Catholic University of Leuven, Belgium. The results were very promising, leaving opened the possibility of future work as the optimization of the correction factors and uncertainties for the dosimeters as well as the applicability of the test objects in evaluating the image quality of digital systems across the country. (author)

  18. Use of different simulators to quality evaluation of image quality in digital mammography; Utilizacao de diferentes simuladores na avaliacao da qualidade da imagem em mamografia digital

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Leslie S.; Coutinho, Celia M.C., E-mail: leslie@ird.gov.br, E-mail: celia@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Magalhaes, Luis A.G.; Almeida, Carlos Eduardo de, E-mail: luisalexandregm@hotmail.com, E-mail: cea71@yahoo.com.br [Universidade do Estado do Rio de Janeiro (LCR/UERJ), Rio de Janeiro, RJ (Brazil). Laboratorio de Ciencias Radiologicas

    2013-11-01

    In this study, the digital images were acquired with different exposure simulators to evaluate the quality of the image, noting the tumor mass detection, microcalcification fiber and representing regions of interest during mammography. The technical parameters of exposure depends on the thickness and composition of the breast, thus affecting the dose and image quality. The simulators were used: ACR, SBP 1054, BREAST PHANTOM CIRS and for evaluation of image quality, as well as measures kerma incident on the entrance surface (Ki) and calculating the mean glandular dose (MGD)

  19. Evaluation of the morphological alteration of the root surface radiated with a diode laser; Avaliacao da alteracao morfologica da superficie cimentaria irradiada com laser de diodo

    Energy Technology Data Exchange (ETDEWEB)

    Gulin, Mauricio

    2003-07-01

    The diode laser has been studied for periodontal therapy, as much for removal of calculus as for microbial reduction of periodontal pockets, as well as the visible analgesic effects and biomodulation capacity. For this reason the purpose of this study was to evaluate the morphological alteration of the root surface after radiation with the diode laser, 808 nm through analysis by scanning electron microscopy (SEM). Besides this, to verify the temperature variations caused during the radiation, a thermometer put into the dentinal wall of the root canal was used. In all, 18 teeth were used, 15 of which for the SEM study, and the other 3 were used to temperature variation analysis. The 25 samples were scraped on the root surface and planed with manual instruments. The other 5 were not subjected to any type of treatment. This, 6 groups of 5 samples each were formed. Control Group C whose samples had not received any treatment; Control Group C 1 was only scraped and polished conventionally with Hu-Friedy Gracey curettes 5 and 6; the other samples groups L1, L2, L3, L4 were radiated by diode laser using parameters of power 1,0 W; 1,2 W; 1,4 W; and 1,6 W respectively, 2 times for 10 seconds with 20 seconds intervals between each radiation in continuous mode. The results with relation to the increase of temperature in the interior of the root canal demonstrated that there was an increase of more than 5 degree Celsius. The results of the scanning electron microscope analysis of Control Group C demonstrated great irregularity and ridges on the root surface, with the presence of a dentine layer. Control Group C1 presented a similar aspect to Group L 1's, smoother and more homogeneous surface. Groups L2, L3, and L4 presented scratches alternating with smoother areas showing that fiber contacted the surface of the sample. The results reconfirmed the necessity of further studies using diode laser, with a beam of light emitted in an interrupted mode to improve the control of the increase of temperature during radiation, and probably followed by a new scraping and root polishing with conventional instruments in an attempt to obtain a smoother and more homogeneous root surface. (author)

  20. Evaluation of the introduction of cogeneration for refrigeration in the fisheries sector of the Amazon; Avaliacao da introducao da cogeracao para refrigeracao no setor pesqueiro do Amazonas

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, Ricardo Wilson Aguiar da [Universidade Estadual do Amazonas (UEA), Manaus (Brazil)], e-mail: rcruz@uea.edu.br; Nebra, Silvia Azucena [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Faculdade de Engenharia Mecanica], e-mail: sanebra@fem.unicamp.br; Cartaxo, Elizabeth Ferreira [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil)], e-mail: ecartaxo@ufam.edu.br

    2004-07-01

    This work analyses the opportunity to introduce cogeneration for the production of aquammonia absorption refrigeration in the autonomous Amazons State power system, by one hand as a means to improve its efficiency and by another, solve the deficiency the State has to store its fish-ing production. (author)

  1. Evaluation of gels obtained from acetylation of chitosan in heterogeneous medium; Avaliacao de geis obtidos a partir da acetilacao da quitosana em meio heterogeneo

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Rosangela Balaban; Silva, Dayse Luzia Pinheiro da; Costa, Marta [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. de Quimica]. E-mail: balaban@digi.com.br; Raffin, Fernanda Nervo [Universidade Federal do Rio Grande do Norte (UFRGN), Natal, RN (Brazil). Centro de Ciencias da Saude. Dept. de Quimica, Tecnologia Farmaceutica e de Alimentos; Ruiz, Naira Machado da Silva [Centro de Pesquisa Leopoldo A. Miguez de Mello (CENPES), Rio de Janeiro, RJ (Brazil)

    2008-07-01

    Chitosan was acetylated during 2, 5 and 10 h and physical gels were obtained at different polymer concentrations in N,N-dimethylacetamide containing 5% of LiCl. Acetylation was confirmed by infrared spectroscopy and {sup 13}C NMR, and degrees of acetylation in the range of 0.82-0.91 were determined by NMR. The O-acetylation degree (0.12-0.15) was exclusively determined by a volumetric method. Rheological studies showed that the storage modulus values were smaller for the more acetylated samples and increased with the temperature and the polymer concentration. All the gels presented storage modulus superior to loss modulus, evidencing more elastic than viscous characteristics. The results obtained in this work suggest a gelation process based on a balance between O and N-acetylation and intermolecular bonds. (author)

  2. Quality assessment of radiopharmaceuticals in nuclear medicine services at Northeast states, Brazil; Avaliacao da qualidade de radiofarmacos em servicos de medicina nuclear de estados da regiao nordeste

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Wellington Gomes de

    2012-07-01

    The radiopharmaceuticals are used in the field nuclear medicine services (NMS) as tracer in the diagnoses and treatment of many diseases. Radiopharmaceuticals used in nuclear medicine and usually have a minimum of pharmacological effect. The procedures for labelling Radiopharmaceuticals should be observed in order to minimize risks to patients, employees and individuals from the public, and to be administered in humans, must be sterile and free of pyrogens and possess elements all measures of quality controls required a conventional drug. The 'Agencia Nacional de Vigilancia Sanitaria (ANVISA)' in its 'Resolucao de Diretoria Colegiada' (RDC) No. 38 of June 4{sup th} 2008, decided that the NMS must perform quality control in the generators eluate and radiopharmaceuticals according to recommendations of manufacturers and scientific evidence accepted by ANVISA. Thus, this study proposes to evaluate the quality of the generator {sup 99M}o-{sup 99m}Tc eluate and radiopharmaceuticals labeled with {sup 99m}Tc used in most NMS of some states in the Northeast, in relation to radionuclide, chemical, radiochemical purity and pH and promote the inclusion of procedure for quality control of radiopharmaceuticals in routine NMS. The results show that 90% radionuclidic purity, 98.2% purity chemical and radiochemical purity of 46% and 100% of the eluates are in agreement with international pharmacopoeias; already radiopharmaceuticals showed 82.6% purity and all radiochemical pH values are also in accordance with international pharmacopoeias. Even with so many positive results, staff the majority of MNS was not able to perform the quality control of the eluates and radiopharmaceuticals. Showing the importance of implementing of quality control programs of the eluates and radiopharmaceuticals in nuclear medicine. (author)

  3. Ribeira do Iguape basin water quality assessment for drinking water supply; Avaliacao da qualidade da agua na bacia hidrografica do Ribeira de Iguape com vistas ao abastecimento publico

    Energy Technology Data Exchange (ETDEWEB)

    Cotrim, Marycel Elena Barboza

    2006-07-01

    Ribeira do Iguape Basin, located in the Southeast region of Sao Paulo state, is the largest remaining area of Mata Atlantica which biodiversity as rich as Amazon forest , where the readiness of water versus demand is extremely positive. With sparse population density and economy almost dependent on banana agriculture, the region is still well preserved. To water supply SABESP (Sao Paulo State Basic Sanitation Company). Ribeira do Iguape Businesses Unit - RR, uses different types of water supplies. In the present work, in order to ascertain water quality for human consumption, major and minor elements were evaluated in various types of water supply (surface and groundwater's as well as the drinking water supplied). Forty three producing systems were monitored: 18 points of surface waters and treated distributed water, 10 points of groundwater and 15 points of surface water in preserved areas, analyzing 30 elements. Bottom sediments (fraction < 63 {mu}m) were also evaluated. The sampling period covered dry and wet seasons from March 2002 to February 2003. The descriptive analysis showed that Al, Fe and Mn, exceeding CONAMA 357 quality guideline. A comparison of the elemental concentrations with the Brazilian Drinking Water Legislation (Portaria 518/04) showed that with the exception of some violations, the levels of all the elements investigated were below the Brazilian Legislation maximum allowed concentrations. This study examined the relationship between the type of water supply and the quality of water used, showed different characteristics on Ca, Fe, Mn concentration. In bottom sediments (fraction <63 {mu}m), Al, Fe and Mg largest concentrations were found. Pb and Zn presents concentrations up to 142,0 {mu}g.g{sup -1} and 172 {mu}g.g{sup -1}, respectively. Data revealed that trace elements concentration in the sediment were below PEL (Probable Effect Level - probable level of adverse effect to the biological community), exception for Pb in Sete Barras and Eldorado. (author)

  4. Avaliação fitoterápica da Jatropha gossypiifolia L. na cicatrização de suturas na parede abdominal ventral de ratos Phytotherapic evaluation of Jatropha gossypiifolia L. on rats ventral abdominal wall wound healing

    Directory of Open Access Journals (Sweden)

    José Ulcijara Aquino

    2006-01-01

    Full Text Available INTRODUÇÃO: A espécie vegetal Jatropha gossypiifolia L., conhecida também como pião roxo, é utilizada na medicina popular como cicatrizante, anti-hipertensivo, purgativo e diurético. OBJETIVO: Avaliar a influência da administração intraperitoneal da Jatropha gossypiifolia L., na cicatrização de suturas da parede abdominal ventral de ratos, observando-se os seus aspectos macroscópicos, tensiométricos e microscópicos. MÉTODOS: Foram utilizados no procedimento 40 ratos da linhagem Wistar, machos, distribuídos em dois grupos de 20. Após incisão da parede e abertura da cavidade abdominal, foi instilado 1 ml/kg/peso de cloreto de sódio a 0,9% no grupo controle e no grupo Jatropha o extrato bruto etanólico da Jatropha gossypiifolia L., na concentração de 1 ml/kg/ peso. Realizou-se a sutura da parede abdominal com fio de polipropileno, com pontos separados. Os animais foram avaliados na sua evolução pós-operatória e mortos em dois subgrupos, no 3º e 7º dia. Analisou-se a parede abdominal ventral macroscopicamente, mediu-se a força de resistência a tensão e foram estudados os aspectos histológicos do reparo cicatricial. RESULTADOS: No exame macroscópico encontraram-se aderências mais intensas nos subgrupos Jatropha no 3º e 7º dia. A avaliação tensiométrica foi significantemente maior nos subgrupos Jatropha no 3º e 7º dia. A avaliação histológica comparativa entre os subgrupos demonstrou que o processo inflamatório agudo foi significantemente maior no subgrupo Jatropha no 3º e 7º dia; a neoformação capilar foi significantemente maior no 3º dia pós-operatório do subgrupo Jatropha sendo os outros parâmetros histológicos semelhantes. CONCLUSÃO: O uso do extrato bruto de Jatropha gossypiifolia L. intraperitoneal não demonstrou melhora significativa no processo de cicatrização da sutura da parede abdominal ventral de ratos com a dose e concentração utilizadas.INTRODUCTION: The Jatropha gossypiifolia

  5. Histomorfometria da mama de ratas tratadas com estrogênio e/ou progestagênio Breast histomorphometry of rats treated with estrogen and/or progestogen

    Directory of Open Access Journals (Sweden)

    Sueli Maria Preda dos Santos Torres

    2011-04-01

    Full Text Available OBJETIVO: Avaliar as alterações histomorfométricas nas mamas de ratas tratadas com estrogênio e/ou progestagênio por curto período de tempo. MÉTODOS: Foram divididas em quatro grupos 40 ratas ooforectomizadas: GC-recebeu veículo; GE-recebeu benzoato de estradiol (37,6 µg/animal; GP-recebeu acetato de medroxiprogesterona (11,28 mg/animal e, GEP-recebeu benzoato de estradiol (37,6 µg/animal e acetato de medroxiprogesterona (11,28 mg/animal. No grupo GE, o estradiol foi administrado durante sete dias, por via subcutânea. Já no grupo EP o estradiol foi administrado nos primeiros sete dias e o progestagênio por mais 23 dias, por via subcutânea. Vinte e quatro horas após a última administração dos hormônios, os animais foram anestesiados e o primeiro par de mamas inguinais removido, imerso em formaldeído a 10% e processado para inclusão em parafina, sendo os cortes corados pela Hematoxilina-Eosina. Foram avaliadas a morfologia e a área ocupada pelo parênquima mamário, sendo os dados submetidos à análise de variância complementado pelo teste de Kruskal-Wallis (p GEP > GC; p OBJECTIVE: To evaluate the breast histomorphometric changes in rats treated with estrogen and/or progestogen for a short period of time. METHODS: Forty oophorectomized rats were divided into four groups: GC, vehicle; GE, treated with estradiol benzoate (37.6 mg/animal; GP, treated with medroxyprogesterone acetate (11.2 mg/animal and GEP, treated with estradiol benzoate (37.6 mg/animal plus medroxyprogesterone acetate (11.28 mg/animal. In GE group, estradiol was administered subcutaneously for seven days; in GEP group, estradiol was administered once in a day for the first seven days and the progestogen over the next 23 days both subcutaneously. Twenty-four hours after the last hormone administration, the animals were killed upon deep anesthesia and the first inguinal breasts were removed, fixed in 10% formaldehyde and processed to be included in paraffin

  6. Obstructive jaundice induces early depression of mitochondrial respiration in rat hepatocytes A icterícia obstrutiva induz depressão precoce da respiração mitocondrial em hepatócitos de ratos

    Directory of Open Access Journals (Sweden)

    Riad Naim Younes

    2007-08-01

    Full Text Available INTRODUCTION: Oxidative phosphorylation dysfunction of hepatocyte mitochondria is involved in the pathophysiology of organ dysfunction following obstructive jaundice (OJ. However the time period from biliary occlusion to the occurrence of the dysfunction has not been determined decisively. PURPOSE: To evaluate the early effects (1 d and 7 d of OJ on liver mitochondria respiratory function in rats. METHODS: Male Wistar rats (200-250 g were randomly divided into the following 3 groups: laparotomy plus OJ for 24 h (1d group (n = 10; laparotomy plus OJ for 7 d (7d group (n = 10; sham control procedure (CTR group (n = 12. At the end of OJ periods, total serum bilirubin level, hepatic enzyme activity levels (GOT, GTP, Gama-GT, ALP, mitochondrial respiration phases S3 and S4, as well as the respiratory control ratio (RC = S3/S4, and ADP consumption/oxygen consumption (ADP/O ratio, were determined. RESULTS: Total serum bilirubin, activity of most hepatic enzymes, and O2 consumption during basal (S4 respiration were increased in the 1d and 7d groups (ANOVA, p = 0.05 vs. CTR. After ADP addition, the O2 consumption rate (S3 in the 1d group remained similar to the CTR rate (ANOVA p > .05, while the RC rate was reduced (ANOVA, p = 0.001 vs. CTR. The effects observed on mitochondrial respiration in the 1d group were exacerbated in the 7d group. CONCLUSION: These results indicate that OJ induces early (24 h depression of liver mitochondria respiration, and thus may lead to early reduction in the production of high energy bonds.INTRODUÇÃO: A disfunção da fosforilação oxidativa das mitocôndrias do hepatócito está envolvida na fisiopatologia da disfunção orgânica subseqüente à icterícia obstrutiva (IO. Entretanto, a precocidade da ocorrência desta disfunção permanece obscura. OBJETIVO: Avaliar o efeito precoce da IO na função respiratória mitocondrial em ratos. MÉTODOS: Ratos Wistar machos (200 a 250g foram randomizados em 3 grupos que foram

  7. Histological study on acute inflammatory reaction to polyurethane-coated silicone implants in rats Estudo histológico da reação inflamatória aguda ao implante de silicone revestido com poliuretano em ratos

    Directory of Open Access Journals (Sweden)

    Paulo Roberto da Silva Mendes

    2008-02-01

    Full Text Available PURPOSE: Evaluating histologically the silicone peri-implant coated by polyurethane inflammation associated to the use of anti-microbial and bacterial contamination. METHODS: It was used 35 Wistar rats. The animals were divided in seven groups: I - Control; II - implant cavity contamination with10 bacteria/ml; III - implant cavity contamination with 10 bacteria/ml; IV - implant cavity contamination with 10 bacteria/ml; V - identical contamination to group II and implant immersions in anti-microbial solution; VI - identical contamination in group III and implant immersions in the anti-microbial solution; VII - identical contamination of group IV and implant immersions in anti-microbial solution. It was evaluated morphometrically the peri-implant capsules after 30 days of introduction. RESULTS: The factors with more discriminating power were the giants cells of a strange body and the mononuclear. There was no correlation between the bacterial concentrations and the histological alterations. CONCLUSION: 1 The histological standard of the inflammatory reaction around the silicone implant coated with polyurethan is chronic granulomatosis type of a strange body; 2 There isn´t correlation between concentration of Staphylococcus epidermidis and histological changes; 3 The use of anti-microbial solution decreased the mononuclear cell reactions, with the increase of giant cells in a strange body.OBJETIVO: Avaliar, histologicamente, a reação inflamatória aos implantes de silicone revestidos por poliuretano, com contaminação bacteriana, associada ou não ao uso de antimicrobianos. MÉTODOS: Utilizou-se 35 ratos Wistar. Os animais foram divididos em 7 grupos: I- Controle, II- contaminação da cavidade do implante com 10¹ bactérias/ml, III- contaminação da cavidade do implante com 10³ bactérias/ml, IV- contaminação da cavidade do implante com 10(5 bactérias/ml, V- contaminação idêntica ao grupo II e imersão dos implantes em solu

  8. Hematoma intraneural experimental em ratos: avaliação da recuperação funcional e histomorfometria neural Experimental intraneural hematoma model in rats: evaluation of functional recovery and neural histomorphometry

    Directory of Open Access Journals (Sweden)

    Gean Paulo Scopel

    2007-01-01

    Full Text Available A formação do hematoma intraneural com comprometimento de nervos periféricos pode ocorrer após trauma ou coagulopatias. O tratamento expectante ou abordagem cirúrgica descompressiva ainda não estão definidos. Foram estudados 40 ratos da raça Wistar divididos em 4 grupos. No grupo A realizou-se injeção intraneural de 0,2 ml de sangue autógeno no nervo ciático direito. No grupo B, após produção do hematoma, fez-se descompressão imediata com epineurotomia longitudinal. No grupo D realizamos liberação imediata do hematoma com neurólise interfascicular. No grupo C (CONTROLE apenas realizou-se a exposição do nervo ciático sem formação de hematoma. A avaliação da função nervosa foi feita com o Índice de Função Ciática de Bain-Mackinnon-Hunter (IFC. O grupo A apresentou IFC inicial de -28,43, com recuperação funcional no 5º dia. A drenagem imediata desse hematoma através de uma epineurotomia longitudinal (B determinou retorno da função ciática normal desde o 1º dia (IFC= -14,42. Tratando o hematoma através da neurólise interfascicular observamos um IFC inicial de -23,69 e retorno da função ciática normal no 3º dia. Os parâmetros histomorfométricos apontaram melhora dos parâmetros de isquemia após os 2 tipos de intervenção cirúrgica aplicadas neste estudo. Não houve diferença entre os tipos de intervenções aplicadas.Emergence of intraneural hematoma with involvement of peripheral nerves can occur after trauma or coagulation disorders. The decision for expectant management or decompressive surgical techniques is still controversial. Forty male Wistar rats were divided into 4 groups. In group A, an intraneural injection of autologous blood was provided at the right sciatic nerve. In group B, after the hematoma creation, a longitudinal epineurotomy was performed. In the group C (sham-operated, the sciatic nerve was exposed without hematoma. In group D. immediately after the hematoma creation, an

  9. The effects of the novel DA D3 receptor antagonist SR 21502 on cocaine reward, cocaine seeking and cocaine-induced locomotor activity in rats.

    Science.gov (United States)

    Galaj, E; Ananthan, S; Saliba, M; Ranaldi, Robert

    2014-02-01

    There is a focus on developing D3 receptor antagonists as cocaine addiction treatments. We investigated the effects of a novel selective D3 receptor antagonist, SR 21502, on cocaine reward, cocaine-seeking, food reward, spontaneous locomotor activity and cocaine-induced locomotor activity in rats. In Experiment 1, rats were trained to self-administer cocaine under a progressive ratio (PR) schedule of reinforcement and tested with vehicle or one of three doses of SR 21502. In Experiment 2, animals were trained to self-administer cocaine under a fixed ratio schedule of reinforcement followed by extinction of the response. Then, animals were tested with vehicle or one of the SR 21502 doses on cue-induced reinstatement of responding. In Experiment 3, animals were trained to lever press for food under a PR schedule and tested with vehicle or one dose of the compound. In Experiments 4 and 5, in separate groups of animals, the vehicle and three doses of SR 21502 were tested on spontaneous or cocaine (10 mg/kg, IP)-induced locomotor activity, respectively. SR 21502 produced significant, dose-related (3.75, 7.5 and 15 mg/kg) reductions in breakpoint for cocaine self-administration, cue-induced reinstatement (3.75, 7.5 and 15 mg/kg) and cocaine-induced locomotor activity (3.75, 7.5 and 15 mg/kg) but failed to reduce food self-administration and spontaneous locomotor activity. SR 21502 decreases cocaine reward, cocaine-seeking and locomotor activity at doses that have no effect on food reward or spontaneous locomotor activity. These data suggest SR 21502 may selectively inhibit cocaine's rewarding, incentive motivational and stimulant effects.

  10. Autonomic modulation of arterial pressure and heart rate variability in hypertensive diabetic rats Modulação autonômica da pressão arterial e variabilidade da freqüência cardíaca em ratos hipertensos e diabéticos

    Directory of Open Access Journals (Sweden)

    Vera de Moura Azevedo Farah

    2007-01-01

    Full Text Available OBJECTIVE: The aim of the present study was to evaluate the autonomic modulation of the cardiovascular system in streptozotocin (STZ-induced diabetic spontaneously hypertensive rats (SHR, evaluating baroreflex sensitivity and arterial pressure and heart rate variability. METHODS: Male SHR were divided in control (SHR and diabetic (SHR+DM, 5 days after STZ groups. Arterial pressure (AP and baroreflex sensitivity (evaluated by tachycardic and bradycardic responses to changes in AP were monitored. Autoregressive spectral estimation was performed for systolic AP (SAP and pulse interval (PI with oscillatory components quantified as low (LF:0.2-0.6Hz and high (HF:0.6-3.0Hz frequency ranges. RESULTS: Mean AP and heart rate in SHR+DM (131±3 mmHg and 276±6 bpm were lower than in SHR (160±7 mmHg and 330±8 bpm. Baroreflex bradycardia was lower in SHR+DM as compared to SHR (0.55±0.1 vs. 0.97±0.1 bpm/mmHg. Overall SAP variability in the time domain (standard deviation of beat-by-beat time series of SAP was lower in SHR+DM (3.1±0.2 mmHg than in SHR (5.7±0.6 mmHg. The standard deviation of the PI was similar between groups. Diabetes reduced the LF of SAP (3.3±0.8 vs. 28.7±7.6 mmHg2 in SHR, while HF of SAP were unchanged. The power of oscillatory components of PI did not differ between groups. CONCLUSIONS: These results show that the association of hypertension and diabetes causes an impairment of the peripheral cardiovascular sympathetic modulation that could be, at least in part, responsible for the reduction in AP levels. Moreover, this study demonstrates that diabetes might actually impair the reduced buffer function of the baroreceptors while reducing blood pressure.OBJETIVO: O objetivo do presente estudo foi investigar a modulação autonômica do sistema cardiovascular em ratos espontâneamente hipertensos (SHR e diabéticos por estreptozotocina (STZ, avaliando a sensibilidade do reflexo barorreceptor e a variabilidade da pressão arterial e da

  11. Influência da ingestão de espirulina sobre o metabolismo de ratos exercitados Influencia de ingestión de Spirulina sobre el metabolismo de ratones exercitados Influence of spirulina intake on metabolism of exercised rats

    Directory of Open Access Journals (Sweden)

    Gustavo Puggina Rogatto

    2004-08-01

    Full Text Available No presente estudo foram comparadas as respostas metabólicas agudas ao exercício em ratos alimentados com dieta padrão e à base de espirulina. Ratos Wistar jovens foram divididos em dois grupos de acordo com a dieta: controle (C (dieta padrão e espirulina (S (dieta à base de espirulina. Ao final do período experimental (cinco semanas os animais foram submetidos a uma sessão aguda de exercício de natação (20 minutos, suportando sobrecarga equivalente a 5% do peso corporal para avaliação do lactato sanguíneo, glicose, insulina, proteínas, albumina e ácidos graxos livres (AGL séricos. Amostras do músculo gastrocnêmio e fígado foram utilizadas para determinação dos teores de glicogênio e lipídeos. Ambos os grupos C e S apresentaram aumento da glicemia e dos AGL, queda da insulinemia e redução dos teores de glicogênio muscular e hepático pós-exercício. A lactacidemia durante o exercício foi superior no grupo S em relação ao C. Conclui-se que o padrão de respostas ao exercício agudo dos grupos C e S foi semelhante. Contudo, a proteína da dieta pareceu influenciar aspectos do metabolismo glicídico.En el presente estudio fueron comparadas las respuestas metabólicas agudas al ejercicio en ratones alimentados con dieta padrón y a base de Spirulina. Ratones Wistar jovenes fueron divididos en dos grupos de acuerdo con la dieta: control (C (dieta padrón y Spirulina (S (dieta a base de Spirulina. Al final del período experimental (5 semanas los animales fueron sometidos a una sesión aguda de ejercício de natación (20 minutos, soportando sobrecarga equivalente a 5% de su peso corporal para evaluación del lactato sanguíneo, glucosa, insulina, proteínas, albumina y ácidos grasos libres (AGL séricos. Las muestras del músculo gastrocnemio y fígado fueron utilizadas para la determinación de los tenores de glicogeno y de lípidos. Ambos grupos C e S presentaron un aumento de la glicemia y de los AGL, mantenimiento

  12. Comparative study of the hyperbaric hyperoxygenation in ischemic colonic loops in rats Estudo comparativo da hiperoxigenação hiperbárica em alças cólicas isquêmicas em ratos

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    Antonio Angelo Rocha

    2007-04-01

    Full Text Available PURPOSE: To analyze and to evaluate the effect of the hyperbaric hyperoxygenation in the mechanical resistance of ischemic colon of rats. METHODS: Eighty rats, distributed in four groups of 20 animals in each one, were used. In group 1 (G1, the control group, ischemia was not caused. Group 2 was submitted to the lesser degree of ischemia. Group 3 was submitted to the intermediate degree of ischemia. In group 4, a bigger degree of intestinal ischemia was provoked. Each group was divided in two sub-groups of ten animals each: with hyperbaric chamber (CC and without hyperbaric chamber (SC. The animals of the four CC subgroups were placed in an experimental hyperbaric chamber in order to inhale oxygen at 100%, at two Absolute Atmospheres, for 120 minutes, for a four-day consecutive period. The animals of the four SC subgroups were kept in environment air during the five days of the experiment. All animals have been submitted to the mechanical study of the intestinal loop by the pressure test of the rupture by liquid distension. The euthanasia occurred in the fifth post-operative day. RESULTS: Considering the ischemia factor, the four groups were different among them (p=0.0001. There was no statistical difference between subgroups CC and SC (p=0.3461. CONCLUSION: The hyperbaric oxygen-therapy did not present improvement on the induced ischemia in rats upright colic loop.OBJETIVO: Analisar e avaliar os efeitos da hiperoxigenação hiperbárica na resistência mecânica do cólon isquêmico de ratos. MÉTODOS: Foram utilizados 80 ratos distribuídos em quatro grupos de 20 animais. No grupo 1, grupo controle, não se provocou isquemia. O grupo 2 foi submetido ao grau menor de isquemia. O grupo 3 foi submetido ao grau intermediário de isquemia. No grupo 4, provocou-se grau maior de isquemia intestinal. Cada grupo foi dividido em dois subgrupos de dez animais cada: com câmara hiperbárica (CC e sem câmara hiperbárica (SC. Os animais dos quatro

  13. Estudo da fração inspirada de oxigênio na isquemia-reperfusão pulmonar em ratos Study of ventilation with different inspired oxygen concentration on lung ischaemia-reperfusion injury in rats

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    Rafael José Silveira

    2004-10-01

    Full Text Available OBJETIVO: Estudar o efeito das frações inspiradas de oxigênio (FiO2 a 0,21, 0,40 e 1,00 na isquemia-reperfusão pulmonar. MÉTODOS: Foram utilizados 40 ratos Wistar, distribuídos aleatoriamente em quatro grupos. O grupo I foi o controle e, nos grupos II, III e IV, os animais foram ventilados durante a isquemia-reperfusão com FiO2 a 0,21, 0,40 e 1,00 respectivamente. O modelo utilizado foi de isquemia-reperfusão normotérmica, in situ. O tempo de isquemia foi de 30 minutos e o de reperfusão, de 10 minutos. Como parâmetros de avaliação, utilizou-se a pressão arterial média sistêmica (PAM, a relação entre a pressão parcial de oxigênio e a fração inspirada de oxigênio (PO2/FiO2, a dosagem da glutationa reduzida (GSH e das substâncias reativas ao ácido tiobarbitúrico (TBARS no tecido pulmonar e a relação entre o peso pulmonar úmido e o peso pulmonar seco. RESULTADOS: Os resultados mostraram que a ventilação com FiO2 a 0,21, quando comparada à ventilação com FiO2 a 0,40 e 1,00, durante o período de isquemia-reperfusão, apresentou menor diminuição da PAM, melhor relação PO2/FiO2, maior valor na medida da GSH, menor produção das TBARS e menor formação de edema pulmonar. CONCLUSÃO: A ventilação com baixa FiO2 (0,21 mostrou melhores resultados quando comparada àquelas realizadas com FiO2 mais elevadas (0,40 e 1,00 na isquemia-reperfusão pulmonar.PURPOSE: To evaluate the FiO2 effect at 0,21, 0,40 and 1,00 on the lung ischaemia-reperfusion injury. METHODS: Forty Wistar rats were randomly allocated in 4 groups. The group I was the control one, and in groups II, III, IV rats were ventilated during the ischaemia-reperfusion at 0,21, 0,40 and 1.00 FiO2 respectively. The ischaemia time was 30 minutes and the reperfusion time was 10 minutes. The model used was normothermic ischaemia-reperfusion, in situ. As assessment parameters, the systemic average arterial pressure (PAM, the oxygen arterial partial pressure

  14. Effects of the cafeteria diet on the salivary glands of trained and sedentary Wistar rats=Efeitos da dieta de cafeteria sobre as glândulas salivares de ratos Wistar sedentários e treinados

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    Sonia Lucy Molinari

    2012-01-01

    Full Text Available The objective of this work was to study the effect of the aerobic physical training and the cafeteria diet introduced after weaning of Wistar rats and on the morphology of the main salivary glands (parotid, submandibular, sublingual. Male rats after weaning were subjected to the cafeteria diet or the standard rodent chow, and either performed aerobic physical training in a treadmill for 100 days, or did not performed any physical activity. Analyses were done considering the response in body weight, adipose tissues and salivary glands, and the data were submitted to statistical treatment (p O objetivo deste trabalho foi avaliar as características morfológicas das glândulas salivares (parótida, submandibular, sublingual apresentadas por ratos submetidos a treinamento físico aeróbio e dieta de cafeteria após o período de lactação. Ratos machos após a lactação consumiram dieta de cafeteria ou ração-padrão para roedores e realizaram treinamento físico aeróbio em esteira rolante por um período de 100 dias, ou não realizaram nenhuma atividade física. Foram feitas análises sobre a resposta do peso corporal, dos tecidos adiposos e das glândulas salivares e os dados submetidos a tratamento estatístico (p < 0,05. A análise morfológica e morfométrica das glândulas salivares foi realizada a partir de cortes histológicos corados com Hematoxilina e Eosina. Apesar do comportamento normofágico, os roedores alimentados com dieta de cafeteria apresentaram maior quantidade de gordura corporal, com repercussão sobre o peso da glândula parótida. A análise morfométrica das glândulas submandibulares indicou redução na proporção dos ácinos serosos como efeito da dieta de cafeteria e do treinamento físico. No entanto, excesso de gordura corporal, dieta de cafeteria e/ou treinamento físico não influenciou a organização histológica das glândulas salivares. Concluiu-se que as glândulas parótidas e submandibulares são mais

  15. Nail and bone surface as indicators of acute exposure to fluoride in rats Unha e superfície óssea como indicadores da exposição aguda em ratos

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    Marília Afonso Rabelo Buzalaf

    2004-12-01

    Full Text Available This study evaluated the use of nails and bone surfaces as indicators of acute exposure to fluoride in rats. Six groups (n=10/grp, aged 70 days, received, by gastrogavage, single doses of sodium fluoride containing 10, 35, 45, 60, 75 and 90 mg fluoride/kg body weight. The control group received deionized water. Two hours after fluoride administration, the rats were killed and their plasma, nails (halves near to the growth end and femur were collected. Nail and plasma fluoride concentrations were analyzed with the electrode following HMDS-facilitated diffusion. Femur surface fluoride was removed from a circular area (4.52 mm² by immersion in 0.5M HCl for 15 seconds, buffered with TISAB and analyzed with the electrode. Data were analyzed by ANOVA and Tukey's test (pEste estudo avaliou o uso de unhas e superfícies ósseas como indicadores da exposição aguda ao flúor em ratos. Seis grupos (n=10/grp, com idade de 70 dias, receberam, por gastro-gavagem, doses únicas de fluoreto de sódio contendo 10, 35, 45, 60, 75 e 90 mg flúor/kg peso corpóreo. O grupo controle recebeu água deionizada. Duas horas após a administração de flúor, os ratos foram mortos e seu plasma, unhas (metades próximas à porção de crescimento e fêmur foram coletados. As concentrações de flúor na unha e plasma foram analisadas com o eletrodo após difusão facilitada por HMDS. O flúor da superfície do fêmur foi removida de uma área circular (4,52 mm² por imersão em HCl 0,5 M por 15 segundos, tamponado com TISAB (contendo NaOH e analisado com o eletrodo. Os dados foram analisados por ANOVA e teste de Tukey (p<0,05 e por regressão linear (p<0,001. As concentrações médias de flúor variaram de 55,4 a 91,5 mg/g para as unhas; de 0,019 a 6,937 mg/mL para o plasma e de 617 a 2.394 mg/g para a superfície do fêmur. A concentração de flúor nas unhas do grupo experimental não foi diferente do controle. Quanto ao flúor no plasma, todos os grupos

  16. Resposta da paratireóide de ratas às variações do cálcio e fósforo plasmáticos no hipertireoidismo e hipogonadismo Response of parathyroid to the plasmatic of calcium and phosphorus variations in hyperthyroidism and hypogonadism in the rat

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    R. Serakides

    2005-02-01

    Full Text Available Foram estudadas 84 paratireóides de ratas Wistar com cinco meses de idade, castradas ou não, e mantidas em hipertireoidismo por períodos de 30, 60 e 90 dias. Dois grupos eutireóideos, um castrado e o outro não, foram mantidos nas mesmas condições e serviram de controle. Ao final de cada período, foram colhidos o plasma, para determinação da concentração de T4 livre, o cálcio e o fósforo e as paratireóides, para análise morfológica e determinação da porcentagem de núcleo, citoplasma e estroma. Aos 90 dias houve reversão da hipocalcemia observada aos 60 dias nos animais eutireóideos castrados e não castrados, graças à hipertrofia da paratireóide. O mesmo não ocorreu com os grupos hipertireóideos que apresentaram hipocalcemia e hiperfosfatemia progressivas e não compensadas até os 90 dias. Na castração há pronta reversão da hipocalcemia em resposta ao aumento da atividade funcional da paratireóide. No estado hipertireóideo com gônadas funcionais, apesar da hipertrofia da paratireóide, não há retorno à isocalcemia e isofosfatemia. Na associação hipertireoidismo-castração, a paratireóide não responde satisfatoriamente à hipocalcemia e hiperfosfatemia intensas e progressivas.Eighty four parathyroids of castrated and intact adult female Wistar rats kept under hyperthyroidism state for 30, 60 or 90 days were studied. Two euthyroid goups, one castrated and another intact were used as controls. At the end of each period, blood was collected and the concentrations of free T4, calcium and phosphorus were determined. The parathyroids were analysed microscopically and % of nuclei, cytoplasm and stroma were determined. Hypocalcemia that was observed in castrated and intact euthyroid rats at 60 days was reverted after 90 days due to parathyroid hypertrophy. In hyperthyroid groups, hypocalcemia and hyperphosphatemia were progressive and did not revert at 90 days. In conclusion, castration revert hypocalcemia due to

  17. Anatomical features of the urethra and urinary bladder catheterization in female mice and rats. An essential translational tool Características anatômicas da cateterização da uretra e bexiga de camundongos e ratos fêmeas. Instrumento essencial na pesquisa pré clínica

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    Leonardo Oliveira Reis

    2011-01-01

    Full Text Available PURPOSE: To present fundamental anatomical aspects and technical skills necessary to urethra and urinary bladder catheterization in female mice and rats. METHODS: Urethral and bladder catheterization has been widely utilized for carcinogenesis and cancer research and still remains very useful in several applications: from toxicological purposes as well as inflammatory and infectious conditions to functional aspects as bladder dynamics and vesicoureteral reflux, among many others. RESULTS: Animal models are in the center of translational research and those involving rodents are the most important nowadays due to several advantages including human reproducibility, easy handling and low cost. CONCLUSIONS: Although technical and anatomical pearls for rodent urethral and bladder access are presented as tackles to the advancement of lower urinary tract preclinical investigation in a broaden sight, restriction to female animals hampers the male microenvironment, demanding future advances.OBJETIVO: Apresentar aspectos anatômicos fundamentais e habilidades técnicas necessárias para cateterismo da uretra e bexiga em ratos e camundongos fêmeas. MÉTODOS: Cateterismo vesical tem sido amplamente utilizado na pesquisa do câncer e carcinogênese, além de várias outras aplicações, desde fins toxicológicos, condições inflamatórias e infecciosas até aspectos funcionais como a dinâmica vesical e refluxo vesico-ureteral, entre muitos outros. RESULTADOS: Os modelos animais estão no centro da investigação de translação e os roedores são os mais importantes devido a várias vantagens, incluindo reprodutibilidade humana, o fácil manuseio e baixo custo. CONCLUSÕES: Apesar de permitir o desenvolvimento da investigação pré-clínica do trato urinário inferior, o modelo se restringe aos animais do sexo feminino, de modo que avanços futuros são necessários.

  18. Fluoxetine effects on mitochondrial ultrastructure of right ventricle in rats exposed to cold stress Efeitos da fluoxetina sobre a ultraestrutura mitocondrial no ventrículo direito de ratos expostos ao estresse pelo frio

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    Fernanda V. Daud

    2009-06-01

    Full Text Available OBJECTIVE: To assess fluoxetine effects on mitochondrial structure of the right ventricle in rats exposed to cold stress. METHODS: The experimental study procedures were performed in 250-300g male EPM-Wistar rats. Rats (n=40 were divided into four groups: 1 Control group (CON; 2 Fluoxetine (FLU; 3 Induced hypothermia (IH and; 4 Induced hypothermia treated with fluoxetine (IHF. Animals of FLU group were treated by the administration of gavages containing 0.75 mg/kg/day fluoxetine during 40 days. The induced hypothermia was obtained by maintaining the groups 3 and 4 in a freezer at -8ºC for 4 hours. The animals were sacrificed and fragments of the right ventricle (RV were removed and processed prior to performing electron microscopic analysis. RESULTS: The ultrastructural changes in cardiomyocytes were quantified through the number of mitochondrial cristae pattern (cristolysis. The CON (3.85%, FLU (4.47% and IHF (8.4% groups showed a normal cellular structure aspect with preserved cardiomyocytes cytoarchitecture and continuous sarcoplasmic membrane integrity. On the other hand, the IH (34.4% group showed mitochondrial edema and lysis in cristae. CONCLUSION: The ultrastructural analysis revealed that fluoxetine strongly prevents mitochondrial cristolysis in rat heart, suggesting a protector effect under cold stress condition.OBJETIVO: Analisar os efeitos da fluoxetina sobre a estrutura mitocondrial do ventrículo direito de ratos expostos ao estresse pelo frio. MÉTODOS: Os procedimentos do estudo foram realizados em ratos Wistar-EPM (250-300g machos. Os ratos (n=40 foram divididos em quatro grupos: 1 Controle (CON; 2 Fluoxetina (FLU; 3 Induzidos à hipotermia (IH e; 4 Induzidos à hipotermia tratados com fluoxetina (IHF. O grupo FLU foi tratado com gavagem contendo 0,75 mg/kg/dia de fluoxetina durante 40 dias. O estresse induzido pelo frio foi realizado mantendo os grupos 3 e 4 em um freezer (-8ºC por quatro horas. Os animais foram sacrificados

  19. Normal microbiota of the perialveolar region of incisors of rats Microbiota normal da região perialveolar de incisivos em ratos

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    F.R.G. Araújo

    2007-12-01

    Full Text Available Identificou-se a microbiota normal da região peri-alveolar de incisivos em 72 ratos Wistar, com 70-90 dias de idade e 280-330g de peso. As bactérias foram coletadas com suabes embebidos em solução salina. Do material depositado em tubo contendo 460µl de Brain Heart Infusion e diluído em 1:10, retirou-se 1µl para semeadura em placas de Petri. O crescimento médio foi 1,4x10(6 ± 2,6x10(5UFC/ml. Segundo a ordem das freqüências, as bactérias encontradas foram: Bacillus sp., Staphylococcus aureus, Streptococcus viridans, Corynebacterium sp., Staphylococcus coagulase negativa, Enterococcus sp., Staphylococcus saprophyticcus, Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae e Serratia liquefaciens. De todas as bactérias isoladas, as Gram-positivas e as Gram-negativas compreenderam 91,2% e 8,8%, respectivamente. Dentre as Gram-positivas, a mais freqüente foi Bacillus sp.(31,2% e a menos, Staphylococcus saprophyticcus (3,0%. Quanto às bactérias Gram-negativas, a mais encontrada foi Escherichia coli (50,1% e a menos, Serratia liquefaciens (6,2%.

  20. Efeitos do ultra-som terapêutico sobre o crescimento longitudinal do fêmur e da tíbia em ratos Effects of therapeutic ultrasound on longitudinal growth of the femur and tibia in rats

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    Alfredo Alcantara Barreto

    2011-01-01

    Full Text Available OBJETIVO: Estudar efeitos do ultra-som terapêutico sobre o crescimento do fêmur e da tíbia, em ratos jovens. MÉTODO: Ratus norvegicus com quatro semanas de vida, machos, totalizando 115 animais, divididos em quatro grupos, foram submetidos ao ultra-som terapêutico (0,8 MHz, cabeçote fixo, pulso contínuo, por dez minutos, durante dez dias, na face medial do joelho direito, nas potências de 0,0 W/cm2 (grupo controle, 0,5 W/cm2 (grupo G2, 1,0 W/cm2 (grupo G3, e 1,5 W/cm2 (grupo G4. Lâminas histológicas da epífise, placa de crescimento e metáfise e as medidas dos comprimentos do fêmur e da tíbia foram estudadas na sexta, décima terceira e vigésima sexta semanas de vida. Os dados foram submetidos à análise de variância - fatorial inteiramente aleatorizado. RESULTADO: Nenhuma alteração estatística do crescimento ósseo foi estabelecida entre quaisquer dos três grupos tratados e o grupo controle. Entretanto, alterações sugestivas de diminuição do crescimento do fêmur e da tíbia de G4 em relação a G2 e G3, foram evidenciadas. No grupo G4 alterações histopatológicas como necroses celulares e neoformação óssea, pós-necrose, foram encontradas. CONCLUSÃO: Quando comparados os grupos tratados com o grupo controle, não há evidência estatística de estímulo ou inibição ao crescimento ósseo pela aplicação do ultra-som terapêutico. Nível de Evidência: Nível II, estudo coorte transversal.OBJECTIVES: To determine the effects of ultrasound therapy on the femur and tibia growth in young rats. METHOD: Four-week-old male Ratus Norvegicus totaling 115 animals, divided into four groups, were submitted to ultrasound therapy (0.8 MHz, fixed tube head, continuous pulse, for 10 minutes, once a day, ten times on the medial face of the right knee, with powers of 0.0 W/cm2 (group G1, 0.5 W/cm2 (group G2, 1.0 W/cm2 (group G3, and 1.5 W/cm2 (group G4. Histological slides of the epiphysis, growth plate and metaphysis and the

  1. Estudo da morfo e citodiferenciação da glândula submandibular remanescente de ratos após excisão parcial de um de seus lobos Study of the morpho and cytodifferentiation of the submandibular gland of rats submitted to partial excision of one of its lobes

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    Anna C. M. Fossati

    2004-06-01

    Full Text Available Manter uma boa saúde depende, entre outros fatores, de um fluxo salivar adequado1-3. Os danos causados por obstrução, traumas ou remoção cirúrgica das glândulas salivares poderão acarretar alteração na produção salivar. Qualquer diminuição no fluxo salivar trará conseqüências danosas ao organismo. Desenvolveu-se este trabalho com o objetivo de aprofundar o conhecimento nos mecanismos que envolvem a regeneração da Glândula Submandibular (GSM de ratos submetida à excisão parcial de um de seus lobos. FORMA DE ESTUDO: Experimental. MATERIAL E MÉTODO: Foram utilizadas lâminas referentes ao desenvolvimento glandular de ratos aos 15, 16, 17, 18 e 19 dias de vida fetal, e 20 ratos machos de 30 ou 60 dias. Após conveniente anestesia foi removido o terço inferior do lobo esquerdo da GSM de cada animal. Após a remoção, foram deixados a se recuperar por 2, 3, 7 e 15 dias. Nestes tempos determinados foram eutanaziados, as glândulas removidas, fixadas em Methacarn, incluídas em parafina, e cortes de 5 ¼m efetuados. Foi realizada coloração ou com hematoxilina/eosina ou com ácido Periódico Reativo de Schiff (PAS. RESULTADOS: Observou-se que o processo regenerativo se instalou precocemente e em todos os espécimes estudados. Foi semelhante ao aspecto verificado no desenvolvimento glandular normal e mais acentuado no rato de 30 dias. A citodiferenciação representada pela marcação das mucinas neutras pelo PAS foi discreta inicialmente, logo após atingindo sua produção máxima, então finalmente reduzindo a expressão, sendo mudada a sua localização inicial. CONCLUSÃO: Baseados nos resultados obtidos, concluiu-se que o processo regenerativo da GSM de ratos excisada é estável, permanente e gradual, seguindo etapas definidas.Maintaining good health depends on, amongst other factors, of an adequate salivary flow1,2,3. Damage caused by obstruction, trauma or surgical removal of the salivary glands may lead to alterations

  2. Correlation of catecholamine levels in the bed nucleus of the stria terminalis and reduced sexual behavior in middle-aged male rats.

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    Chen, Joyce C; Tsai, Houng-Wei; Yeh, Kuei-Ying; Tai, Mei-Yun; Tsai, Yuan-Feen

    2008-07-01

    The correlation between dopamine (DA) and norepinephrine (NE) levels in the bed nucleus of the stria terminalis (BNST) and male sexual behavior was examined in middle-aged rats. Male rats (18-19 months) were divided into: (a) Group MIE, consisting of rats showing mounts, intromissions, and ejaculations; (b) Group MI, composed of rats showing mounts and intromissions, but no ejaculation; and (c) Group NC, consisting of noncopulators. Young adult rats (4-5 months) displaying complete copulatory behavior were used as the control. Tissue levels of DA, NE, and DA metabolites in the BNST were measured by high-pressure liquid chromatography. DA, but not NE, levels in MIE rats were significantly lower than those in young controls. DA and NE levels in MIE rats were significantly higher than those in NC rats. These results suggest that DA and NE in the BNST might play an important role in the control of male sexual behavior in middle-aged rats.

  3. Radionecrosis attenuation in wistar rats with cutaneous application of quercetin; Atenuacao da radionecrose em ratos Wistar com aplicacao cutanea de quercetina

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Nelson Mendes

    2016-11-01

    The increased incidence of cancer has been significant in recent decades in the world population, as confirmed by national and international institutions in the health area. The emergence of cancer is influenced, predominantly by genetic and environmental factors, being manifested more in the adult population. The main modalities for cancer treatment (radiotherapy, chemotherapy and surgery) may be used separately or in combination, depending on the type of cancer. Among the methods mentioned, radiation therapy is the one more broadly used for the treatment of patients, having an associated side effect called radiodermatitis, which has degrees of severity ranging from simple erythema to radionecrosis. The manifestation of radiodermatitis may occur during the treatment or after the radiotherapy sessions: both situations have great relevance in the patient's quality of life and social costs. One of the studied alternative therapies for attenuating the radionecrosis is the quercetin cutaneous application. One of the alternative therapies, studied to mitigate or eliminate the radionecrosis, is based on the topical application of quercetin. To evaluate the effectiveness of this mitigation, an animal model of radionecrosis was developed, to be used in Wistar rats. After in vitro studies, the quercetin concentrations and time of application were determined, reducing the number of animals, when in vivo experiments are carried out. With the topical application of 250 μ mol/L of quercetin, one hour prior to gamma irradiation, at a dose of 85 Gy, the side effects of radiation were minimized, avoiding the formation of radionecrosis. There was, also, a tendency to attenuate the wound area in the studied animals, compared to the irradiated animals without the quercetin application. (author)

  4. O processo de cicatrização influenciado pelo hipotireoidismo e pelo envelhecimento: estudo da cicatrização de anastomoses intestinais, em ratos The healing inffluenced by hypotireoidism and elderly: study of intestinal anastomosis healing in rats

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    Maria de Lourdes Pessole Biondo-Simões

    2005-01-01

    baixa da síntese e retardo de maturação das cicatrizes das anastomoses muito mais às custas do hipotireoidismo do que do envelhecimento.TURPOSE: The aging of the population has led to an increased number of interventions in elderly patients, with hypothyroidism, even at the subclinical level, being a common situation among these patients. The objective of the present study was to determine the influence of hypothyroidism on the healing process of colon anastomoses in aged rats. METHODS: 96 male Wistar rats were used, 48 of them young and 48 old. Half the young animals and half the old ones were euthyroid and half were hypothyroid. Hypothyroidism was obtained by total thyroidectomy. Thirty days after surgery the animals were submitted to median laparotomy followed by transverse colotomy of the left colon with end-to-end anastomosis and laparorrhaphy. Six animals from each group were submitted to euthanasia by drawing lots on the 3rd, 7th and 14th postoperative day and submitted to macroscopic, resistance, and histopathological analysis of the anastomoses. RESULTS: No dehiscence was observed and the gain of resistance and the general histological evolution in terms of epithelialization and inflammatory reaction was similar in all groups. Analysis of total collagen content revealed that the anastomoses of young and old animals showed the same behavior on the 3rd day, although less collagen was observed in hypothyroid animals (YN x YH p=0.0000; ON x OH p=0.0000. This behavior persisted on the 7th and 14th day. Less collagen I was present in the anastomoses of hypothyroid animals on the 3rd (YN x YH p=0.0015; ON x OH p=0.0000, 7th (YN x YH p=0.0006; ON x OH p=0.0001 and 14th day (YN x YH p=0.0181; ON x OH p=0.0057, the same occurring for collagen III on the 3rd (x YH p=0.0007; ON x OH p=0.0260, 7th (YN x YH p=0.0160; ON x OH p=0.2670 and 14th day (YN x YH p=0.0000; ON x OH p=0.0030. CONCLUSION: Analysis of the results permitted us to conclude that lower collagen

  5. Effects of hyperglycemia and aging in angiogenesis and reepithelization of colonic anastomoses in rats Efeitos da hiperglicemia e do envelhecimento sobre a angiogênese e a reepitelização de anastomoses colônicas em ratos

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    Norton Luiz Nóbrega

    2007-01-01

    Full Text Available PURPOSE: To evaluate angiogenesis and reepithelization of colonic anastomoses in rats. METHODS: 82 Wistar rats, divided into: young normoglycemic, old normoglycemic and hyperglycemic. Diabetes was induced with streptozotocin. Glycemia was assessed before induction, at 24 hours and after 90 days, when a colotomy and an anastomosis were performed, assessed at days 3, 7, and 14. Samples were prepared by immuno-histochemistry (PCNA and antifactor VIII. RESULTS: Mean glycemia after 90 days streptozotocin induction was 244,95 mg/dl. Day 7, reepithelization was greater in the young group than in the old normoglycemic (pOBJETIVO: Avaliar a angiogênese e a reepitelização de anastomoses colônicas em ratos. MÉTODOS: 82 ratos Wistar divididos em: jovens normoglicêmicos, velhos normoglicêmicos e hiperglicêmicos. Diabetes foi induzido com estreptozotocin. Glicemia foi medida antes da indução, após 24 horas e 90 dias, quando realizou-se colotomia seguida de anastomose, a qual foi estudada no 3.º, 7.º e 14.º dia.Peças foram preparadas por imunohistoquímica (PCNA e Antifator VIII. RESULTADOS: Glicemia média após 90 dias foi de 244,95 mg/dl. No 7.º dia, a reepitelização foi maior no grupo jovem que nos grupos velho normoglicêmico (p<0,0001 e velho hiperglicêmico (p<0,0001. Dia 14, mantiveram-se as diferenças entre os grupos jovem e velhos normoglicêmico (p<0,0001 e hiperglicêmico (p<0,0001. Os dois grupos velhos não diferiram entre si. Nos três períodos a angiogênese foi maior no grupo jovem do que nos velhos normoglicêmicos (p3=0.014, p7<0.0001; p14<0.0001 e que nos velhos hiperglicêmicos (p3=0.014, p7<0.0001; p14<0.0001. No 3.º dia, os grupos velhos não foram diferentes (p3=0.627, mas no 7.º e no 14.º, a angiogênese foi maior no grupo normoglicêmico (p7=0.042; p14=0.005. CONCLUSÃO: Idade é importante para a reepitelização e angiogênese das anastomoses colônicas. Hiperglicemia interfere principalmente na angiogênese.

  6. Efeito da desnutrição na cicatrização de anastomoses colônicas: estudo experimental em ratos Effects of malnutrition on colonic anastomosis healing: an experimental study in rats

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    Manuela Molina Ferreira

    2006-09-01

    Full Text Available A deiscência de anastomose é uma das mais graves complicações advindas de operações do tubo gastrintestinal. Algumas condições gerais podem prejudicar o processo de cicatrização, tais como: desnutrição e hipoalbuminemia. Este estudo experimental teve como objetivo avaliar a cicatrização de anastomoses colônicas na vigência de desnutrição protéico-calórica e hipoalbuminemia. Dividimos os animais em dois grupos, sendo um deles o controle e o outro desnutrido (ingestão diária de metade da ração do grupo controle por vinte dias. O peso corporal, a albumina sérica, a evolução clínica, a cavidade abdominal, os aspectos macro e microscópicos da anastomose, a esteatose hepática e a concentração tecidual de hidroxiprolina foram observadas em cada animal. Pudemos notar que o método utilizado para desnutrir os animais mostrou-se eficaz, uma vez que houve redução significativa do peso do grupo experimental. Observamos que o grupo desnutrido apresentou dados necroscópicos de pior prognóstico e mortalidade superior ao grupo controle. Concluímos que a desnutrição influencia negativamente na cicatrização de anastomoses colônicas e aumenta significativamente a mortalidade.Dehiscence of colonic anastomosis is one of the most severe complications after gastrointestinal surgery. The intestinal anastomosis healing complication is associated to several factors like malnutrition and hypoalbuminemia. This is an experimental study that aimed to evaluate the colonic anastomosis healing in malnutrition and hypoalbuminemia conditions. Animals were separated in two experimental groups: control and submitted to malnutrition (daily food intake as half of the control group per 20 days. Body weight, clinical outcome, serum albumin, abdominal cavity aspects, gross and microscopic aspects of the anastomosis, hepatic steatosis and tissue hydroxyproline dosage were compared between groups. Our results show that malnutrition development was

  7. Efeito de um hidrolisado de proteínas de soro de leite e de seus peptídeos na proteção de lesões ulcerativas da mucosa gástrica de ratos Effects of a whey protein concentrate and it's peptides in the protection of ulcerative lesions at rat gastric mucosa

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    Maria Teresa Bertoldo Pacheco

    2006-02-01

    Full Text Available OBJETIVO: Avaliar a atividade do hidrolisado das proteínas de soro de leite bovino e uma fração de peptídeos de baixo peso molecular (peso molecular OBJECTIVE: To assess the ability of bovine whey protein hydrolysate and its low molecular weight fraction (molecular weight <1kDa to protect the gastric mucosa of rats against ulcerative process induced by three different agents. METHODS: Adult Wistar rats were subjected to the indomethacin-induced ulcer (30mg/kg body weigh, absolute ethanol (1ml/animal and immobilization and cold stress (4(0C/2h, models. RESULTS: Whey protein hydrolysate was obtained by treatment with pancreatin to a degree of hydrolysis of 20% and fractionated using a tangential flow membrane with a molecular weight cut-off of 1kDa to obtain the fraction containing low molecular weight peptides (<1kDa. In the ethanol-induced acute ulcer model (single dose, whey protein hydrolysate inhibited the gastric lesion index by 65.5% and the double dose resulted in a 77.4% inhibition. CONCLUSION: For the anti-inflammatory model, the cytoprotective effect of low molecular weight peptides was stronger than that of total hydrolysate (53.1 and 71.6%, ulcerative lesion index for single and double dose, respectively. No mucosa cytoprotective activity was found for whey protein concentrate, whey protein hydrolysate or WPHP in the immobilization and cold stress model.

  8. Embryogenesis-promoting factors in rat serum.

    Science.gov (United States)

    Katoh, M; Kimura, R; Shoji, R

    1998-06-15

    Regarding whole rat embryo cultures in vitro, rat serum as a culture medium is known to support the normal growth of rat embryos in the organogenesis phase. The purpose of the present study was to isolate the embryogenesis-promoting factors from rat serum as a first step in the development of a defined serum-free medium for a whole embryo culture system. Pooled rat serum after heat inactivation was fractionated into three major peaks (frA, containing a region of void volume, frB, and frC) by gel filtration. The 9.5-day rat embryos that were cultivated for 48 hr in essential salt medium containing frB (with a molecular size range of 100-500 kDa) revealed normal growth. Three proteins (27 kDa, 76 kDa, and 190 kDa) that had the embryogenesis-promoting effects were isolated from 3-hr delayed centrifuged rat serum by the ion exchange chromatography. The 76-kDa protein was found to be rat transferrin by immunoblotting. The 27-kDa protein was identified as apo-AI (the major apoprotein of high-density lipoprotein) by immunoblotting. High-density lipoprotein obtained from pooled rat serum by a NaBr density gradient ultracentrifugation was found to have a positive effect on embryogenesis. The 10-kDa protein was also identified as alpha 1-inhibitor 3 by immunoblotting. In addition, the embryogenesis-promoting effect of the fraction containing 27-kDa and 190-kDa proteins declined within a short period of storage at -20 degrees C. This decrease was countered by supplementing its fraction (D-2) with albumin isolated from rat serum. These results in the present study suggest that transferrin, high-density lipoprotein, and alpha 1-inhibitor 3 in rat serum may be embryogenesis-promoting factors, and that albumin appeared to play a role in the embryogenesis of rat embryos in whole embryo cultures.

  9. Análise clínica e biomecânica do efeito do diclofenaco sódico na consolidação da fratura da tíbia no rato Clinical and biomechanical analysis of the effect of diclofenac sodium in tibial fracture healing in rats

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    Sérgio Swain Müller

    2004-12-01

    Full Text Available Os AINH (Antiinflamatórios não hormonais são agentes utilizados na prática clínica que interferem no processo inflamatório pela inibição da síntese de prostaglandinas e tromboxanos. Alguns trabalhos experimentais investigaram sua ação no processo de consolidação de fraturas, por meio de estudos clínicos e histológicos, sendo escassas as análises biomecânicas. Nesse estudo foram utilizados 20 ratos da linhagem Wistar, divididos aleatoriamente em dois grupos iguais: grupo A (controle e grupo B (tratado com diclofenaco sódico. Em ambos os grupos foram realizadas fraturas abertas, após perfuração, na tíbia direita. A administração da droga foi via intramuscular, dose única diária, por 28 dias. Os animais foram pesados semanalmente. Após o sacrifício as tíbias foram dissecadas, pesadas e submetidas a ensaio biomecânico de flexão analisando-se carga máxima, deformação e coeficiente de rigidez. Observou-se que no grupo tratado com AINH não houve aumento do peso corpóreo a partir da segunda semana e as tíbias fraturadas foram mais pesadas. Neste grupo o calo ósseo suportou menor carga máxima, apresentando maior deformação e menor coeficiente de rigidez. Nos animais tratados, o osso não fraturado também se mostrou menos rígido. Concluiu-se, nas condições estudadas, que o DS alterou o processo de consolidação e o metabolismo ósseo, levando a retardo na maturação do calo e menor rigidez do osso intacto, respectivamente.The antinflammatories are agents utilized on clinical practice that interfere on inflammatory process by synthesis inhibition of prostaglandin and tromboxanes. Some experimental studies investigated their action on the fractures consolidation process, through clinical and histological studies, as the biomechanical analyses are scarce. In this study, 20 (twenty Wistar pedigree rats were used, aleatory divided into two groups: A group (control and B group (treated with diclofenac. In both

  10. Participação da apoptose na rejeição aguda do transplante intestinal em ratos Apoptosis participation in the acute rejection of intestinal transplantation in rats

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    André Dong Won Lee

    2004-09-01

    Full Text Available RACIONAL: O transplante de intestino delgado é procedimento cirúrgico em estudo visando sua aplicação no tratamento dos pacientes portadores da síndrome do intestino curto, com vistas à reabilitação oral. A grande barreira, porém, se deve à rejeição pela grande quantidade de tecido linfóide presente no intestino delgado. OBJETIVO: Estudo da apoptose em alotransplante heterotópico intestinal. MATERIAL E MÉTODOS: Realizaram-se 24 alotransplantes intestinais em ratos da raça Brown-Norway (doador para Lewis (receptor, sendo subdivididos em três subgrupos de oito animais, sacrificados respectivamente no terceiro dia de pós-operatório (Tx(3, no quinto dia de pós-operatório (Tx(5 e no sétimo dia de pós-operatório (Tx(7 para coleta das biopsias dos enxertos intestinais. Compararam-se os resultados com o grupo isotransplante (C que envolveu oito animais da raça Lewis (doador para Lewis (receptor, porém neste grupo realizaram-se biopsias seriadas no mesmo animal, sendo subdivididos em três momentos: biopsia no terceiro dia de pós-operatório (C(3, no quinto dia de pós-operatório (C(5 e sacrificados no sétimo dia de pós-operatório (C(7 para coleta da biopsia. Realizou-se, inicialmente, análise intragrupo entre os momentos C(3, C(5 e C(7 para todos os parâmetros de rejeição citados anteriormente, como também para os três subgrupos Tx(3, Tx(5 e Tx(7. Posteriormente, realizou-se a análise intergrupo de forma transversal e pareada comparando-se o grupo isotransplante com o grupo alotransplante. (C(3 com Tx(3; C(5 com Tx(5 e C(7 com Tx(7. No grupo isotransplante não houve expressão estatística quanto aos marcadores analisados. Porém, no grupo alotransplante observou-se que alterações da apoptose foram marcantes a partir do terceiro dia de pós-operatório.BACKGROUND: Intestinal transplantation is a possible treatment for patients with short bowel syndrome, aiming the reintroduction of oral diet. However, the major

  11. Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model Influência da laparoscopia e laparotomia na gasometria, leucócitos e citocinas em modelo de sepse abdominal em ratos

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    Irami Araújo Filho

    2006-04-01

    Full Text Available PURPOSE: Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change cytokine expression, gas parameters and leukocyte count in septic rats. METHODS: Wistar rats were randomly assigned to five groups: control (anesthesia only, laparotomy, CO2 pneumoperitoneum, cecum ligation and puncture by laparotomy, and laparoscopic cecum ligation and puncture. After 30 min of the procedures, arterial blood samples were obtained to determine leukocytes subpopulations by hemocytometer. TNFalpha, IL-1beta, IL-6 were determined in intraperitoneal fluid (by ELISA. Gas parameters were measured on arterial blood, intraperitoneal and subperitoneal exsudates. RESULTS: Peritoneal TNFalpha, IL-1beta and IL-6 concentrations were lower in pneumoperitoneum rats than in all other groups (pOBJETIVO: A cirurgia laparoscópica está associada com trauma reduzido e baixa resposta na fase aguda do trauma, quando comparada com a cirurgia aberta. As citocinas e o balanço ácido-base são fatores importantes da resposta biológica ao trauma cirúrgico-anestésico. O objetivo deste estudo foi determinar se o pneumoperitôneo com CO2 altera a expressão das citocinas, a gasometria e a contagem diferencial de leucócitos em ratos com sepse abdominal. MÉTODOS: Ratos Wistar foram aleatoriamente distribuídos em 5 grupos: controle (somente anestesia, laparotomia, pneumoperitôneo com CO2, ligadura e punção do ceco por laparotomia, ligadura e punção do ceco por laparoscopia. Após 30 minutos dos procedimentos, sangue arterial foi colhido para leucometria diferencial em hemocitômetro. TNFalfa, IL-1beta e IL-6 foram dosadas no líquido intraperitoneal (por ELISA. Os parâmetros gasosos foram medidos no sangue arterial e nos exsudatos

  12. Efeito da bebida de café descascado sobre a atividade antioxidante, os parâmetros hematológicos e bioquímicos em ratos Peeled coffee brew effect in the antioxidant activity hematological and biochemical parameters in rats

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    Stella Maris da Silveira Duarte

    2009-12-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da bebida filtrada, preparada com café-arábica descascado, sobre a peroxidação de lipídios, os parâmetros hematológicos e bioquímicos in vivo. Para isso foram utilizados ratos que ingeriram 280 mg/kg/dia da bebida de café por 7 dias (tratamento agudo e 30 dias (tratamento crônico. A fim de determinar se a bebida de café é capaz de reduzir o estresse oxidativo, foi analisada a peroxidação de lipídios isolados de cérebro de rato analisando-se as substâncias reativas do ácido tiobarbitúrico. A ingestão da bebida por 7 e 30 dias inibiu significativamente a peroxidação lipídica (p The aim of this study was to verify the effect of filtered coffee brew prepared with peeled Coffea arabica on the lipid peroxidation and on hematological and biochemical parameters in vivo. Rats were ingested 280 mg/kg/day of coffee brew for 7 days (acute treatment and 30 days (chronic treatment. In order to determine whether coffee can reduce the oxidative stress, the rats brain isolated lipid peroxidation was accessed evaluating the thiobarbituric acid-reactive substances (TBARs.The ingestion of coffee brew for 7 and 30 days inhibited, significantly, the malondialdehyde concentration (p 0.05 between the percentage of peroxidation inhibition when the acute (48.6% and chronic (53.4% treatments were compared. The chronic ingestion of moderate doses of filtered coffee brew did not modify the plasma level of the hematological and biochemical parameters analyzed. The results indicate the beneficial health effect of moderated filtered coffee brew ingestion since it inhibited lipid peroxidation, but did not change the rats cholesterol and triacylglycerol plasma levels.

  13. Efeitos da suplementação oral com creatina sobre o metabolismo e a morfologia hepática em ratos Effects of creatine oral supplementation on the hepatic metabolism and morphology of rats

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    Rodolfo de Paula Vieira

    2008-02-01

    Full Text Available A creatina é uma amina nitrogenada e tem sido utilizada principalmente por atletas e praticantes de atividade física que desejam aumentar a massa muscular e o desempenho físico. Entretanto seu uso não está somente relacionado à prática esportiva, pois inúmeros trabalhos apresentam efeitos benéficos na prática médica. Alguns estudos demonstraram que a suplementação oral com creatina resulta em aumento da sua biodisponibilidade plasmática e também de seus estoques em inúmeros órgãos. Entretanto, estudos sobre possíveis efeitos tóxicos da suplementação com creatina são escassos. Portanto, o objetivo deste trabalho foi avaliar os possíveis efeitos tóxicos da suplementação oral com creatina sobre a função e morfologia hepáticas em ratos após 14 dias de suplementação oral com creatina na dose de 0.5 g/kg/dia. A função hepática foi avaliada através de testes bioquímicos e a estrutura hepática foi avaliada através da massa hepática relativa e da análise histológica. Os resultados demonstraram que 14 dias de suplementação com creatina não alteraram a função hepática quando comparado os grupos controle e suplementado: AST (39.5 x 44.4 U/L, ALT (18.6 x 30.8 U/L, ALP (38.5 x 31.4 U/L, GGT (134.8 x 143.8 U/L, proteínas totais (5.1 x 5.5 g/dl, triglicérides (141.0 x 141.0 mg/dl, colesterol total (130.1 x 126.2 mg/dl, colesterol LDL (36.1 x 36.1 mg/dl, colesterol HDL (65.6 x 62.4 mg/dl, colesterol VLDL (25.0 x 28.0 mg/dl, e também estrutura hepática, exceto nos níveis plasmáticos de albumina (3.0 x 3.5 mg/dl - pCreatine is a nitrogenated amine and it has been used mainly by athletes and physical activity practitioners who wish to increase muscle mass and performance. However its use is not just related to sports practice, once several studies have shown beneficial effects on medical practice. Some studies have demonstrated that oral creatine supplementation increases its plasmatic bioavailability and also

  14. LUCRO, VALOR CONTABIL E DIVIDENDOS NA AVALIAcAO DO PATRIMÔNIO LIQUIDO

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    James A. Ohlson

    2009-09-01

    Full Text Available A contabilidade atribui uma importante função integrativa a demonstração das mutações do patrimônio liquido. A demonstração inclui os itens mais importantes do balanço o e da demonstração de resultado do exercício - o valor do patrimônio liquido e o lucro - e sua forma de apresentação exige que a alteração no valor do patrimônio liquido seja igual ao lucro menos os dividendos (líquidos das contribuições para aumento de capital. Referimo-nos a essa relação corno sendo a “relação de lucro limpo", pois, da forma como foi articulada, todas as alterações nos ativos e passivos que não estejam relacionadas com os dividendos devem passar pela demonstração de resultado do exercício. A teoria da contabilidade adota geralmente esse esquema sem relacioná-lo a perspectiva do usuário dos dados contábeis. Contudo, a idéia fundamental de que estoques (líquidos de valor são compatíveis com a criação c distribuição de valor levanta uma questão básica no contexto de avalia4ao do patrimônio liquido: e possível delinear urna teoria coesiva do valor de uma firma sustentada pela relação de lucro limpo, no intuito de se identificar um papel claro para cada uma das três variáveis, quais sejam: o lucro, o valor contábil do patrimônio liquido e os dividendos?

  15. Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab Análise da força tênsil na cicatrização da parede abdominal de ratos tratados com infliximabe

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    João Vieira Lopes

    2008-10-01

    Full Text Available PURPOSE: To evaluate the effects of infliximab, a murine/human chimeric monoclonal antibody, on the tensile strength of abdominal wall surgical wounds. METHODS: Sixty Wistar healthy male rats with initial body weight between 215 and 390 g and 60 and 90 days of age were randomly assigned into two groups, E (Experimental and C (Control with 30 animals each. Group E animals received a single subcutaneous dose of 5mg/Kg of infliximab, and Group C animals received equivalent subcutaneous volume of a solution of 0.9% NaCl. After 48h, animals from both groups were submitted to a 4 cm median incision in the abdominal wall, including all layers that had been reconstituted with continuous suture of the aponeurotic muscle and skin, with 5.0 nylon thread. Then, Group E animals were separated by simple allotment into three subgroups named E3, E7 and E14 with ten animals each, and those from group C into C3, C7, C14 and were submitted, respectively, the reoperation and euthanasia at the third, seventh and fourteenth postoperative day. The anterior abdominal wall, which was resected during reoperation, was cut with No 15 scalpel lamina perpendicularly to the surgical wound. Each specimen, in the form of a 6 cm x 2 cm strip, was fixed by the extremity so that the suture line was equidistant from the fixation points of the dynamometer, in order to undergo the tensile strength test. The dynamometer, which was gauged for each series of measures, was calibrated to apply velocity to the 25 mm/min rupture test; the rupture value was expressed in N (Newton. Prior to euthanasia, the abdominal vena cava was identified and punctured in order to collect blood for TNF-α dosage. RESULTS: The mean tensile strength found for animals from subgroups E3, E7, E14, C3, C7, C14 were, respectively, 16.03, 18.69, 27.01, 28.40, 27.22, 29.15 and 24.30 N. In the results of the multiple comparisons tests, significant differences (pOBJETIVO: Avaliar os efeitos do infliximabe, anticorpo

  16. Efeito da suplementação de glutamina sobre variáveis hematológicas e do estado nutricional de ratas desnutridas Glutamine supplementation effect in hematological parameters and nutritional status of rats submitted to malnutrition protocol

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    Natália Cristina Lima Rodrigues

    2007-12-01

    Full Text Available RACIONAL: A glutamina é o aminoácido mais abundante no sangue, por desempenhar importante papel no sistema imune. É considerado aminoácido condicionalmente essencial em situações como desnutrição protéico-calórica. OBJETIVO: Verificar o efeito da dieta suplementada com glutamina sobre variáveis bioquímicas e hematológicas de ratas submetidas a protocolo de desnutrição, induzido por dieta rica em lactose. MÉTODOS: Ratas Wistar fêmeas (n=20 e com 21 dias de idade, foram submetidas ou não à desnutrição calórico-protéica induzida por diarréia, através da administração de dieta rica em lactose 60% durante 15 dias. Após o período de desnutrição, animais eutróficos ou desnutridos permaneceram durante 30 dias com oferta de diferentes dietas (padrão e experimentais. De acordo com estado e tratamento nutricionais, os animais foram distribuídos aleatoriamente em quatro grupos distintos: 1 GC: animais eutróficos + dieta padrão, 2 GD: animais desnutridos + dieta rica em lactose (60%, 3 GDGLN: animais desnutridos + dieta rica em glutamina (2%; e 4 GDP: animais desnutridos + dieta padrão. Após os 30 dias de tratamento nutricional, amostras de sangue foram obtidas por punção cardíaca para avaliação de variáveis bioquímicas (proteínas totais, albumina, uréia e hematológicas (quantificação da série vermelha e branca. RESULTADOS: Após 15 dias do início do experimento o ganho de peso dos grupos GD (46,4 ± 2,60g, GDGLN (39,2 ± 8,9g e GDP (33,2 ± 11,5g foi menor em relação ao controle (64 ± 4,24g, PBACKGROUND: Glutamine is the most abundant amino acid in blood stream, playing an important role in the immune system. It is conditionally considered an essential amino acid to certain state conditions such as protein-energy malnutrition. AIM: To verify the effect of glutamine diet supplemented on hematological and biochemical variables in rats subjected to protocol of malnutrition, induced by an enriched lactose

  17. Influência da suplementação de creatina sobre a massa óssea de ratos espontaneamente hipertensos Influence of creatine supplementation on bone mass of spontaneously hypertensive rats

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    Christiano Robles Rodrigues Alves

    2012-06-01

    Full Text Available INTRODUÇÃO: Recentes evidências indicam que a suplementação de creatina (Cr é capaz de aumentar a densidade mineral óssea (DMO no fêmur de ratos saudáveis em crescimento. Entretanto, há poucos estudos que testam a efetividade da suplementação desse nutriente em condições de perda óssea. OBJETIVO: Investigar o efeito da suplementação de Cr na DMO e no conteúdo mineral ósseo (CMO de ratos espontaneamente hipertensos (SHR, um modelo experimental de baixa massa óssea. MATERIAIS E MÉTODOS: Dezesseis ratos SHR machos com 8 meses de idade foram randomizados em dois grupos experimentais pareados pelo peso corporal, a saber: 1 Pl: SHR tratados com placebo (água destilada; n = 8; e 2 Cr: SHR tratados com Cr (n = 8. Após nove semanas de suplementação os animais foram eutanasiados e o fêmur e a coluna vertebral (L1-L4 foram analisados por densitometria óssea (Dual Energy X-Ray Absorptiometry. RESULTADOS: Não houve diferença significativa na DMO (Pl = 0,249 ± 0,003 g/cm² vs. Cr = 0,249 ± 0,004 g/cm²; P = 0,95 e no CMO (Pl = 0,509 ± 0,150 g vs. Cr = 0,509 ± 0,017 g; P = 0,99 da coluna vertebral e na DMO (Pl = 0,210 ± 0,004 g/cm² vs. Cr = 0,206 ± 0,004 g/cm2;P = 0,49 e no CMO (Pl = 0,407 ± 0,021 g vs. Cr = 0,385 ± 0,021 g; P = 0,46 do fêmur total entre os grupos experimentais. CONCLUSÃO: Neste estudo, usando um modelo experimental de baixa massa óssea, a suplementação de Cr não afetou a massa óssea.INTRODUCTION: Recent evidence has suggested that creatine supplementation (Cr can increase the bone mineral density (BMD of the femur in healthy growing rats. Nevertheless, studies assessing the efficacy of the Cr supplementation in conditions characterized by bone mass loss are scarce. OBJECTIVE: To investigate the effect of Cr supplementation on BMD and bone mineral content (BMC in spontaneously hypertensive rats (SHRs, an experimental model of osteoporosis. MATERIALS AND METHODS: Sixteen 8-month-old male SHRs were

  18. Modificações do volume e da histologia de focos de endometriose em ratas tratadas com sinvastatina Changes in the volume and histology of focus of endometriosis in rats treated with sinvastatin

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    João Nogueira Neto

    2007-08-01

    Full Text Available OBJETIVO: analisar alterações macroscópicas e histológicas que ocorrem com o uso da sinvastatina em endometriose experimental em ratas. MÉTODOS: quarenta ratas da linhagem Wistar foram submetidas à técnica de autotransplante uterino em mesentério. Após três semanas, 24 ratas desenvolveram endometriose experimental grau III e foram divididas em dois grupos: Sinvastatina (dado 20 mg/kg/dia via oral e Controle (dado cloreto de sódio a 0,9% na quantidade de 1 mL/100 g de peso corpóreo via oral, que receberam gavagem durante 14 dias seguido de morte. Os volumes dos implantes foram calculados [4pi (comprimento/2 x (largura/2 x (altura/2/3] nas intervenções cirúrgicas e após a morte dos animais. Os autotransplantes foram retirados, corados com a hematoxilina-eosina e analisados à microscopia de luz. Foram usados o teste de Mann-Whitney para amostras independentes e o teste de Wilcoxon para amostras relacionadas. Para avaliação histológica, foi usado o teste exato de Fisher, adotando-se nível de significância de 5%. RESULTADOS: a diferença entre os volumes médios iniciais dos autotransplantes nos dois grupos foi insignificante (p=1,00, e, entre os volumes médios finais, significante (p=0,04. Houve aumento significativo (p=0,01 entre os volumes médios iniciais e finais do Grupo Controle e redução insignificante no Grupo Sinvastatina (p=0,95. Histologicamente (p=0,64, o Grupo Sinvastatina (n=9 mostrou a parede epitelial moderadamente preservada em sete casos (77,80% e dois casos com camada epitelial bem preservada (22,2%, e o Grupo Controle (n=12 com sete casos (58,30% moderadamente preservados e cinco casos (41,70% bem preservados. CONCLUSÕES: a sinvastatina impediu o crescimento dos focos de endometriose experimental. São promissores os estudos com uso da sinvastatina por período mais prolongado.PURPOSE: to analyze the macroscopic and histological changes that occur with the use of sinvastatin in experimental endometriosis

  19. O processo de cicatrização influenciado pelo hipotireoidismo e pelo envelhecimento: estudo da cicatrização da parede abdominal, em ratos The healing process inffluenced by hypotireoidism and by elderly: study of abdominal wall healing in rats

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    Maria de Lourdes Pessole Biondo-Simões

    2005-01-01

    O: A análise dos resultados permite concluir que existe prejuízo da cicatrização na presença de hipotireoidismo que é piorada com o envelhecimento.PURPOSE: Hypothyroidism is a common situation among the aged which affects protein synthesis. The objective of the present study was to determine the influence of hypothyroidism on the healing process in aged rats. METHODS: 96 male Wistar rats were used, 48 of them with a mean age of 110 days and 48 with a mean age of 750 days. Half the young animals and half the old ones were euthyroid and half were hypothyroid. Hypothyroidism was obtained by total thyroidectomy. Thirty days after surgery the animals were submitted to median laparotomy followed by laparorrhaphy on 2 synthesis planes. Six animals from each group were submitted to euthanasia by drawing lots on the 3rd, 7th and 14th postoperative day and submitted to macroscopic, resistance, and histopathological analysis. RESULTS: The resistance of the skin scars was lower in hypothyroid animals, both the young (p=0.0145 and old (p=0.0242 ones, with no significant relation with age being detected. The resistance of the scars on the peritoneum-muscle-aponeurosis plane was lower in old animals on the 24th day (p=0.0014 and even lower in hypothyroid old animals (p=0.0000. The histological evolution was similar, but differences in collagen presence were observed. On the 7th day the skin scars had a lower collagen content in young (p=0.0201 and old (p=0.0003. hypothyroid animals. On the 14th day the scars of old hypothyroid animals had less collagen than those of normal old animals (p=0.0092 and those of young hypothyroid animals (p=0.0283. On the 3rd day, the scars of hypothyroid animals on the peritoneum-muscle-aponeurosis plane presented less collagen than those of both young (p=0.0089 and old (p=0.0171 normal controls, but there was no relation with age. This association persisted on the 7th and 17th day, but age dependence was observed at these time points. CONCLUSION

  20. Nefropatia induzida por contraste: avaliação da proteção pela n-acetilcisteína e alopurinol em ratos uninefrectomizados Contrast-induced nephropathy: evaluation of n-acetylcysteine and allopunirol protective effect in uninephrectomized rats

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    José Carlos Carraro Eduardo

    2008-06-01

    Full Text Available OBJETIVO: A nefropatia por contraste é a terceira causa de insuficiência renal aguda em pacientes hospitalizados. O objetivo deste estudo foi avaliar a ação da n-acetilcisteína e do alopurinol na proteção renal em ratos de ambos os sexos que receberam diatrizoato. MATERIAIS E MÉTODOS: Ratos Wistar adultos jovens, uninefrectomizados e submetidos a restrição hídrica, receberam solução salina (grupo 1: machos; grupo 2: fêmeas, diatrizoato (grupo 3: machos; grupo 4: fêmeas, diatrizoato e n-acetilcisteína (grupo 5: machos, diatrizoato e alopurinol (grupo 6: machos e diatrizoato e n-acetilcisteína + alopurinol (grupo 7: machos. A filtração glomerular foi avaliada pela creatinina. O teste t de Student e o teste do sinal foram utilizados para análises estatísticas. RESULTADOS: Ratos que receberam diatrizoato apresentaram elevação estatisticamente significante da creatinina sérica, quando comparados aos controles, porém não houve diferença entre os sexos. Os animais que receberam alopurinol não mostraram aumento significante da creatinina, enquanto a administração de n-acetilcisteína não impediu a elevação da creatinina. CONCLUSÃO: O alopurinol mostrou-se mais efetivo que a n-acetilcisteína na proteção funcional renal ao dano induzido pelo diatrizoato de sódio. Não houve diferença entre os sexos na intensidade do dano renal pelo diatrizoato de sódio.OBJECTIVE: Contrast medium-induced nephropathy is the third most frequent cause of iatrogenic acute renal failure involving inpatients. The present study was aimed at evaluating the protective effect of n-acetylcysteine and allopurinol in both male and female rats receiving diatrizoate. MATERIALS AND METHODS: Thirty-five young adult Wistar rats submitted to hydric restriction were divided into groups as follows: groups 1 and 2 (respectively male and female rats receiving saline solution; groups 3 and 4 (respectively male and female rats receiving diatrizoate; group 5

  1. Alterações pulmonares em um modelo de diabetes mellitus em ratos: o efeito da terapia antioxidante Lung alterations in a rat model of diabetes mellitus: effects of antioxidant therapy

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    Luiz Alberto Forgiarini Junior

    2010-10-01

    Full Text Available OBJETIVO: Avaliar as alterações estruturais no pulmão de ratos com diabetes mellitus (DM através da quantificação do estresse oxidativo e do dano ao DNA, assim como determinar os efeitos de superóxido dismutase (SOD exógena nessas alterações. MÉTODOS: Estudo experimental controlado com 40 ratos Wistar, divididos em quatro grupos (10 animais cada: grupo controle, grupo SOD (sem DM e tratados com SOD, grupo DM (com DM induzido por estreptozotocina, e grupo DM+SOD (com DM induzido por estreptozotocina e tratados com SOD. Os animais foram avaliados por um período de 60 dias, iniciado a partir do dia em que os animais com diabetes induzido por estreptozotocina apresentaram glicemia > 250 mg/dL. Nos últimos 7 dias do período, os animais nos grupos tratados receberam SOD. Ao final do tempo de estudo, amostras de tecido pulmonar foram coletadas para análise histopatológica e avaliação do estresse oxidativo e do dano ao DNA. RESULTADOS: Não houve diferenças significativas entre os grupos em relação ao dano ao DNA. Houve um aumento significativo na matriz extracelular e hiperplasia do endotélio capilar no grupo DM quando comparado com os grupos controle e SOD. Também houve mudanças significativas em pneumócitos tipo II e macrófagos intravasculares, sugerindo um processo inflamatório no grupo DM. Entretanto, uma redução na matriz extracelular, endotélio capilar normal e pneumócitos tipo II normais foram encontrados no grupo com DM+SOD. CONCLUSÕES: A administração exógena de SOD pode reverter alterações nos pulmões de animais com DM induzido.OBJECTIVE: To evaluate structural alterations of the lung in rats with diabetes mellitus (DM, by quantifying oxidative stress and DNA damage, as well as to determine the effects that exogenous superoxide dismutase (SOD has on such alterations. METHODS: A controlled experimental study involving 40 male Wistar rats, divided into four groups (10 animals each: control; SOD

  2. Influência da administração de extratos de duas plantas calcinogênicas sobre a fertilidade de ratos Effect of calcinogenic plant extracts on rat fertility

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    J.R.B. Mello

    1999-10-01

    Full Text Available Investigou-se o efeito de extratos aquosos de Solanum malacoxylon (Sendter Solanaceae (SM (sin.: S. glaucophyllum e Nierembergia veitchii (Hook Solanaceae (NV, plantas calcinogênicas encontradas no Brasil, sobre a reprodução de ratos e sobre suas progênies. No experimento 1, ratos e ratas Wistar, em número variável conforme o grupo, receberam por via oral, durante os 40 dias anteriores ao acasalamento, extrato aquoso de ambas as plantas em dosagem equivalente à administração diária de 10,9g×kg-1 de planta seca. Os resultados foram comparados com os de um grupo tratado diariamente com vitamina D3 (3,0mg×kg-1 e com os de um grupo-controle (salina. No experimento 2, ratas prenhes receberam, por via oral, três diferentes doses dos extratos de ambas as plantas (equivalente à administração diária de 5,4; 10,9 e 21,7g×kg-1 de planta seca, durante toda a gestação. Os resultados foram comparados com três grupos tratados com vitamina D3 (0,75; 2,25 e 3,75mg×kg-1 e um grupo-controle (salina. Quando administradas antes da gestação, o ganho de peso das fêmeas e a fertilidade foram reduzidos nos grupos tratados com SM (11% e vitamina D3 (11%. No grupo tratado com NV, a fertilidade também foi reduzida (25%, mas o desenvolvimento ponderal durante o tratamento não foi afetado. Nos grupos tratados com SM e vitamina D3 anterior à gestação, o número de filhotes por ninhada foi reduzido, assim como o seu peso ao nascer. Características de desenvolvimento e desempenho físico dos filhotes não foram afetados em nenhum dos grupos. O tratamento das fêmeas com extrato aquoso de SM durante a gestação causou redução significativa e dose-dependente nas características reprodutivas estudadas. O mesmo foi observado com as fêmeas tratadas com vitamina D3. Com o extrato aquoso de NV não foram observadas reduções nas taxas reprodutivas, quando as fêmeas foram tratadas durante a gestação. Com as doses 10,9 e 21,7g×kg-1 de NV foram

  3. Alterações funcionais da junção neuromuscular provocadas em ratos pela administração diária e prolongada de um agente curarizante Functional changes of the neuromuscular junction induced in rats by a daily and prolonged ministration of a curare agent

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    Antonio Carlos Zanini

    1967-06-01

    Full Text Available A ratos adultos, machos, foi administrado durante 8 semanas, uma vez ao dia, o iodeto do dimetiléter da d-tubocurarina (DMT, pela via intraperitoneal. Os animais foram distribuídos em 3 lotes: os do 1.°, receberam 5,5 gama de DMT/kg/dia; os do 2.°, 16,6 gama/kg/dia; os do 3.° (controle, 1 ml da solução isotônica de cloreto de sódio. A força muscular dos animais foi avaliada uma vez por semana, de acordo com o método descrito no texto, antes e após a injeção intraperitoneal da referida substância. Com o decorrer da experimentação, observou-se que: a houve diminuição significativa da força muscular dos animais, avaliada após esvaecimento da atividade curarizante do DMT; b a determinação da força muscular, feita logo após a injeção, mostrou que a dose de 5,5 gama/kg/dia provoca um aumento da suscetibilidade dos animais à ação curarizante do DMT; c o efeito inibidor provocado pela dose de 16,6 gama/kg/dia de DMT aumentou no início mas diminuiu significantemente após a 5ª semana, muito embora houvesse uma progressiva diminuição da força muscular, sugerindo independência entre os dois efeitos. Com base nesses resultados, são discutidos alguns fenômenos que podem ocorrer na instalação e tratamento da miastenia grave.Dimethylether of d-tubocurarine iodide (DMT was administered daily, by intraperitoneal route, to adult male rats during 8 weeks. The animals were divided in three groups: the animals of the first group were given 5.5 gama/kg/day of DMT; those of the second group were given 16.6 gama/ kg/day; the rats of the 3rd (the control group, received 1 cm³ of isotonic saline solution. The animals muscular force was evaluated once a week right before and after intraperitoneal injection of the drug. During the course of the trial, it was noted: aa significant reduction of the animals muscular force, evaluated after disappearance of the DMT curarizing effect; bmeasurements of muscular force, performed straight

  4. The effect of prophylactic dose of a low molecular weight heparin on skin wound healing of rats Efeito da dose profilática de heparina de baixo peso molecular na cicatrização de feridas na pele de ratos

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    Ozdamar Fuad Oken

    2009-12-01

    Full Text Available PURPOSE: To investigate the effect of prophylactic dose of a low molecular weight heparin, enoxaparin, on skin wound healing of rats. METHODS: Forty rats were used for the study. Rats were randomly assigned to two equal groups. Experimental group received prophylactic dose of enoxaparin. Physiologic saline was administered to the control group. Parameters of wound healing of experimental and control groups were compared. For comparison of the groups in terms of fibrosis, vascularization, inflammation, epithelization, and tensile strength test (Newton. Mann-Whitney-U test was used because variables were categorical data (fibrosis, vascularization, inflammation and epithelization. Differences between groups were analyzed with independent samples t-test (tensile strength. Significance was set at pOBJETIVO: Investigar o efeito de dose profilática da heparina de baixo peso molecular, enoxaparina, na cicatrização de feridas na pele de ratos. MÉTODOS: Quarenta ratos foram utilizados para o estudo. Ratos foram distribuídos aleatoriamente a dois grupos iguais. O grupo experimental recebeu profilática de enoxaparina. Solução salina fisiologica foi administrada ao grupo controle. Foram comparados parâmetros de cicatrização dos grupos experimental e controle.Os grupos foram comparados em termos de fibrose, vascularização, inflamação, epitelização e força tensil (teste de Newton. Foi realizado o teste de Mann-Whitney-U para variáveis com dados categóricos (fibrose, cicatrização, inflamação e epitelização. Diferenças entre os grupos foram analisadas como amostras independentes pelo t-teste (força tensil. Significância foi fixada para p < 0,05. RESULTADOS: A ferida do grupo experimental apresentou força tensil diminuída significativamente (p < 0,001, o exame histopatológico revelou um significativo (p < 0,001 retardo na epitelização e diminuição na fibrose, cicatrização, inflamação (p < 0,001 no grupo experimental

  5. Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats Dosagem da proteína carbonilada sanguínea como biomarcador específico do estresse oxidativo após reperfusão intestinal em ratos

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    Márcio José Jamel

    2010-02-01

    Full Text Available PURPOSE: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. METHODS: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. RESULTS: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. CONCLUSION: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.OBJETIVO: Realizou-se um estudo experimental com a finalidade de investigar o uso da proteína carbonilada como um marcador biológico específico do estresse oxidativo em um modelo de isquemia e reperfusão intestinal, em ratos. MÉTODOS: Vinte e quarto ratos da linhagem Wistar, machos foram distribuídos, aleatoriamente, em três grupos compostos por oito animais cada: Grupo 1 - Controle; Grupo 2 - Simulação e Grupo 3 - Submetido à isquemia, mediante clampeamento de ramos ileais da artéria mesentérica superior por uma hora, seguida de reperfusão, por igual período. Amostras sanguíneas obtidas foram utilizadas para analise dos níveis de proteína carbonilada, através do método Slot blotting. RESULTADOS: No grupo 3 houve uma elevação significante da concentração de proteína carbonilada sérica se comparada aos níveis sanguíneos homogêneos encontrados nos grupos 1 e 2. CONCLUSÃO: Fundamentado nos resultados é possível concluir que, a carbonilação protéica pode ser utilizada como um marcador específico para a mensuração do

  6. Histomorfometria e histoquímica da tuba uterina e do útero de ratas púberes e pré-púberes induzidas ao hipertireoidismo Histomorphometry and histochemistry of the uterine tubes and uterus of puberal and prepuberal rats induced for hyperthyroidism

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    T.S. Oliveira

    2005-08-01

    Full Text Available Foram estudadas a histomorfometria e a atividade secretória das tubas uterinas e do útero de 38 ratas Wistar púberes, distribuídas em quatro grupos: (1 eutireóideo em proestro-estro, (2 hipertireóideo em proestro-estro, (3 eutireóideo em metaestro-diestro e (4 hipertireóideo em metaestro-diestro. Posteriormente, foram utilizadas outras 24 ratas Wistar pré-púberes, com 12 dias de idade, distribuídas em dois grupos: (1 hipertireóideo (n=12 e (2 eutireóideo (n=12. O útero e as tubas uterinas foram colhidos para avaliação histomorfométrica e histoquímica. A altura do epitélio da ampola, tanto no proestro-estro quanto no metaestro-diestro, elevou-se com a administração de tiroxina, tornando-se semelhante à do istmo. O hipertireoidismo aumentou a secreção PAS positiva e de mucossubstâncias ácidas do infundíbulo na fase estrogênica, igualando-a à do istmo. A parede uterina apresentou-se mais espessa na fase estrogênica, resultado da ação conjunta da tiroxina no endométrio e no miométrio. A atividade secretória e a composição do colágeno uterino não diferiram entre tratamentos. Nas ratas pré-púberes, o hipertireoidismo aumentou a espessura do miométrio e da parede uterina, mas não alterou a tuba uterina. O hipertireoidismo em ratas pré-púberes induz modificações significativas somente no útero; após a maturidade sexual, as modificações ocorrem no útero e na tuba uterina em intensidade variável e dependentes da fase do ciclo estral.The histomorphometry and the secretory activity of the uterine tubes and uterus of 38 puberal Wistar rats were studied. Rats were divided in four groups: (1 euthyroid in proestrus-estrus, (2 hyperthyroid in proestrus-estrus, (3 euthyroid in metestrus-diestrus and, (4 hyperthyroid in metestrus-diestrus. In other experiment, 24 prepuberal 12-day-old Wistar rats were divided in two groups: (1 hyperthyroid and (2 euthyroid. The uterus and the uterine tubes were collected for

  7. Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura Effects of physical exercise in the Ampkα expression and activity in high-fat diet induced obese rats

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    José Rodrigo Pauli

    2009-04-01

    Full Text Available INTRODUÇÃO: A ingestão de dieta hiperlipídica é um fator de risco singular no desenvolvimento de resistência à insulina e diabetes do tipo 2. OBJETIVO: O estudo investigou os efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos. MÉTODOS: Foram utilizados ratos Wistar, aleatoriamente divididos em quatro grupos, que receberam dieta padrão de manutenção (grupo controle ou dieta hiperlipídica (DHL (grupos sedentários e exercitados, por período de quatro meses. Dois diferentes protocolos de exercícios foram utilizados: exercício agudo ou crônico de natação. O teste de tolerância à insulina foi realizado para estimar a sensibilidade à insulina. Os níveis protéicos da AMPKα e do GLUT4 e também de p-AMPKα e pACC no músculo esquelético dos ratos foram determinados através da técnica de Western blot. RESULTADOS: O teste de tolerância à insulina revelou significativo prejuízo na ação da insulina após a alimentação com a DHL, indicando insulino-resistência quando comparado com grupo controle (p INTRODUCTION: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. OBJECTIVE: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. METHODS: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group or an isocaloric high-fat diet (HFD (sedentary groups and exercised groups for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPKα and GLUT4 as well as p-AMPKα and pACC of rats' skeletal muscle levels were determined using Western blot. RESULTS: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control

  8. Effects of the combined use of glutamine and growth hormone in the intestinal adaptation after massive resection of the small bowel in rats Efeitos do uso combinado da glutamina oral e hormônio do crescimento na adaptação intestinal após ressecção extensa do intestino delgado em ratos

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    Joaquim M. Spadoni

    2005-10-01

    Full Text Available PURPOSE: The aim of this study was to investigate the effects of the combined use of glutamine (GL and growth hormone (GH in the intestine of rats submitted to 80% small bowel resection. METHODS: [24] Twenty four Wistar rats were randomized to receive either a standard rat chow - control group (CG, n=12 or the same diet added to 4% glutamine - GL-GH group (n=12 after 80% enterectomy. The latter group received subcutaneously 0,6UI/day of GH. Groups of six rats in each group were killed on the 5th and 14th days. The following variables were studied: body weight, mucosal weight, histomorphometry and DNA content in the resected specimen and in the adapted intestines after necropsy. RESULTS: All animals lost weight stabilizing after the 5th PO day in both groups. There was not any statistical difference in the mucosal weight associated to groups and dates. However, ileal mucosal weight decreased from basal to final results when compared to jejunal mucosa (p= 0.02. The DNA content increased from the initial to the final results (p OBJETIVO: Investigar os efeitos do uso combinado da glutamina (GL e do hormônio do crescimento (GH no intestino de ratos submetidos a ressecção de 80% do intestino delgado. MÉTODOS: Vinte e quatro ratos Wistar foram randomizados para receber uma a dieta padrão- grupo controle (GC, n=12 ou a mesma dieta adicionada de glutamina 4% (isocalórica, isoproteica - grupo glutamina- hormônio do crescimento (GL-GH, n=12 após a enterectomia à 80%. Este último grupo recebeu por via sub-cutânea, 0,6 UI/dia de GH. Grupos de seis ratos cada foram sacrificados no 5º e 14º dias. As seguintes variáveis foram estudadas: peso corporal, peso de mucosa, histomorfometria e conteúdo de DNA no segmento ressecado inicialmente e no intestino adaptado coletado após o sacrifício. RESULTADOS: Todos os animais perderam peso até o 5º dia, estabilizando-se após esta data em ambos os grupos. Não houve diferença estatística no peso da

  9. Daño renal en ratas inducido por veneno de Bothrops neuwiedii diporus de Argentina Renal injury in rats poisoned by venom of Bothrops neuwiedii diporus from Argentina

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    Patricia Koscinczuk

    2004-08-01

    Full Text Available La insuficiencia renal aguda es una de las complicaciones sistémicas más frecuentes después de un accidente ofídico. En este estudio se evalúan los efectos que el veneno de Bothrops neuwiedii diporus produce en el riñón de ratas machos de la cepa Wistar. La histopatología permitió comprobar el desarrollo de necrosis tubular aguda; las lesiones iniciales se observaron a las 3 horas de la inoculación de una dosis de 700 µg del veneno, observándose en corteza renal congestión y degeneración granulohialina de las células epiteliales tubulares, acompañadas de dilatación y cilindros hialinos en la luz tubular. A las 24 horas se presentó necrosis tubular aguda en una superficie extensa de la corteza sin daño de la membrana basal tubular. Las lesiones de degeneración turbia de células epiteliales tubulares, dilatación de la luz tubular y cilindros hialinos se mantuvieron presentes hasta las 4 semanas post-inoculación. Si bien los parámetros de la bioquímica clínica asociados con insuficiencia renal aguda aumentaron a las 6 horas de la administración del veneno (urea: 1.10±0.22 g/dl; creatinina: 19.60±1.51mg/dl, a la semana descendieron a valores normales. Las densidades urinarias, en cambio, a la semana se mantuvieron más bajas que lo normal, 1.005 ± 0.001 (pAcute renal failure is one of the systemic complications that can be found in bothropic accidents. In this study the effects on male Wistar rats induced by the venom of Bothrops neuwiedii diporus were evaluated. The histopathology revealed acute tubular necrosis, lesions firstly were observed 3 hours post inoculation of 700 µg of venom. Cortical kidney congestion and granulohialin degeneration of tubular epithelial cells were observed, these lesions achieved a maximum at 24 hours after inoculation. Tubular epithelial hidropic degeneration and dilatation of tubular lumen with hyalin casts were present inclusive up to 4 weeks after inoculation. Biochemical parameter

  10. Efeito do extrato bruto hidroalcoólico e de frações de folhas da Averrhoa carambola L. (Oxalidaceae no metabolismo glicêmico de ratos Wistar = Effect of Hydroalcoholic extract and fractions of Averrhoa carambola L. (Oxalidaceae leaves on the glycemic metabolism of Wistar rats

    Directory of Open Access Journals (Sweden)

    Marilene Provasi

    2005-01-01

    Full Text Available As folhas de carambola (Averrhoa carambola L. são utilizadas na fabricação do fitoterápico Glico-Vitae., indicado no tratamento do diabetes melittus tipo 2. Em nossos estudos, ratos machos Wistar que receberam, por via intragástrica, o extrato bruto liofilizado (EC e as frações de EC (20 mg/kg, durante duas semanas, apresentaram redução da glicemia. Mas, diferentemente da insulina, o EC e suas frações não estimularam a síntese de glicogênio e a produção de lactato em músculo sóleo isolado. Da fração mais ativa, empregando métodos espectroscópicos de EM, RMN1H e RMN13C, foram isoladas trêsfrações semipurificadas majoritárias e, por comparação com os dados da literatura, foram identificadas como misturas complexas de açúcares.Carambola (Averrhoa carambola L. leaves have been used for the production of Glico-Vitae., a phytotherapic medicine indicated for type 2 diabetes mellitus. Male Wistar rats, which received intragastric administration of the liophyliled extract (EC and fractions of EC (20 mg/kg during 2 weeks, showed decreased (p < 0.05 blood glucose levels. However, in contrast to insulin, EC and its fractions neither increased the glycogen synthesis or thelactate production in isolated soleus muscle. In addition, by using spectroscopic methods (MS, NMR1H and NMR13C a mixture of complex sugars were obtained from the fraction with the highest activity.

  11. Utilização da multimistura durante a lactação e seus efeitos na produção e composição do leite materno de ratas Use of multimixture during lactation and its effects on production and composition of rat milk

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    Milane Souza LEITE

    2002-08-01

    Full Text Available A multimistura, composta por farelos, pós de folhas verdes, pós de sementes e pó da casca de ovo, vem sendo amplamente recomendada para aumentar a produção de leite materno, porém sem bases científicas que confirmem sua eficácia. Este estudo objetivou avaliar o desempenho lactacional de ratas alimentadas com dieta suplementada com 2% de multimistura (MM. Foram utilizados ratos fêmeas da linhagem Wistar lactantes com idade entre 90 e 100 dias de vida, divididos nos seguintes grupos (n=12: a à base da dieta habitual do Estado do Rio de Janeiro (HERJ; b dieta HERJ suplementada com 2% de multimistura (HERJ+MM; c à base de caseína, com 12% de proteínas (CAS1; d à base de caseína, com 20% de proteínas (CAS2. Foram determinados os macronutrientes proteína, lactose e lipídios e a produção de leite. O grupo HERJ+MM apresentou a menor produção de leite (pThe addition of multimixture, composed of bran, green leaves powder, seeds powder and eggshell powder, to the population's diet has been encouraged in Brazil, as a means to increase the production of breast milk, although without scientific bases that confirm its efficacy. The objective of this study was to evaluate the lactation performance of rats receiving ad libitum diet supplemented with 2% of multimixture (MM. Female Wistar-lactating rats of 90 to 100 days of age were divided into the following groups (n=12: a fed the typical diet of the state of Rio de Janeiro, Brazil (HERJ; b HERJ diet supplemented with 2% of multimixture (HERJ+MM; c casein diet, with 12% of proteins (CAS1; d casein diet, with 20% of proteins (CAS2. Lipid, lactose and protein concentrations and milk production were determined. The HERJ+MM group presented significantly lower milk production (p<0.05 and lower lactose content. In conclusion, there was a decrease in the lactation performance of rats which received diet supplemented with 2% of multimixture.

  12. Action of tacrolimus on Wistar rat kidneys implanted with Walker 256 carcinosarcoma Estudo da ação do tacrolimus em rins de ratos Wistar implantados com carcinossarcoma de Walker

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    Cristiano Machado Inácio

    2010-02-01

    Full Text Available PURPOSE: To evaluate the development of Walker 256 tumor in male Wistar rats treated with tacrolimus using an experimental kidney tumor model. METHODS: 40 male Wistar rats were divided into four groups: Tumor group (TU (n=10, Tacrolimus-Tumor group (TT (n=10, Tacrolimus group (TC (n=10 and Control group (C (n=10. Treatment with tacrolimus was performed in groups TT and TC. Under anesthesia, the right kidney of each animal of TU and TT was accessed through a supraumbilical incision and inoculated with a 0.1mL solution containing 2x10(6 tumor cells (Walker 256 carcinosarcoma tumor cells. Group TC was treated with a saline solution. All the animals of groups TC and TT were treated with tacrolimus (5mg/kg/day by gavage for 15 days. TU group animals received saline by gavage for 15 days. On the 15th postoperative day, all animals were submitted to euthanasia and blood sampling for analysis of serum creatinine (Cr and blood urea nitrogen (BUN. Abdominal gross examination was performed, the right kidney removed and prepared for histological analysis by hematoxylin-eosin staining. The resulting data were submitted to statistical analysis by ANOVA. RESULTS: Statistical significance was found when comparing creatinine level between groups TU, TT and TC -TT group culminated with a marked increased in creatinine levels (Cr=1.013 ± 0.3028 mg/mL, TU group (Cr=0.5670 ± 0.03536 mg/dL P=0.00256, TC group (Cr =0.711 ± 0.1653 mg/mL P= 0.02832. Statistical significance was found when comparing BUN levels in TT group (71.32 ± 17.14 mg/mL, compared with TU group (45.83 ± 5.046 mg/dL, P=0.000318. There were no statistically significant differences between groups TT and TC (61.23 ± 9.503 mg/mL P=0.7242. Histological analysis showed a poor evolution in TT group with multiple foci of hemorrhage and cortical invasion by the Walker tumor. CONCLUSION: The Tacrolimus-treated group developed a more aggressive tumor and a drug-related nephrotoxic effect.OBJETIVO: Avaliar

  13. Efeitos do uso crônico do nelfinavir sobre a prenhez da rata albina: ensaio biológico Effects of chronic nelfinavir treatment on rat pregnancy: biological assay

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    Camila Fernandes Venneri Mathias

    2006-03-01

    Full Text Available OBJETIVO: avaliar o efeito do uso crônico do nelfinavir sobre o peso de ratas albinas prenhes e seus conceptos, bem como o número de implantações, fetos, placentas, reabsorções e mortalidade materna e fetal. MÉTODOS: 50 ratas albinas EPM-1 Wistar, prenhes, foram aleatoriamente divididas em cinco grupos: 2 controles, Contr1 (controle do estresse e Contr2 (controle do veículo, e três experimentais, Exp40, Exp120 e Exp360, que receberam, respectivamente, 40, 120 e 360 mg/kg por dia de nelfinavir por via oral. A droga e o veículo (água destilada foram administrados por gavagem em duas tomadas diárias (12/12 horas, desde o primeiro dia até o dia 20 da prenhez. No último dia do experimento, todos os animais foram anestesiados e sacrificados. Foram avaliados a evolução do peso, número de implantações, reabsorções, fetos, placentas, óbitos intra-uterinos, o peso dos fetos e das placentas e malformações maiores. A análise estatística foi realizada pela análise de variância (ANOVA completada pelo teste de Kruskal-Wallis. RESULTADOS: em relação ao ganho de peso das ratas, houve ganho normal em todos os grupos, não sendo constatadas diferenças significantes entre eles. ANOVA mostrou ausência de diferenças significativas entre os grupos quanto aos parâmetros estudados. As médias do número de fetos foram: controles = 9,7±0,50; grupos tratados com nelfinavir = 9,7±0,81. Para as médias de números de placentas e implantações, controles = 9,7±0,50; grupos tratados com nelfinavir = 9,7±0,78. Quanto às médias de pesos fetais, controles = 4,04±0,50; grupos tratados com nelfinavir = 3,91±0,33 g. Finalmente, para as médias de pesos de placentas, controles = 0,64±0,02; grupos tratados com nelfinavir = 0,67±0,02 g. Além disto, não foram observadas reabsorções, mortalidade das matrizes, óbitos e malformações fetais. CONCLUSÕES: o nelfinavir, em todas as doses administradas, não influiu no ganho de peso das

  14. Activity, distribution and regulation of phosphofructokinase in salivary gland of rats with streptozotocin-induced diabetes Atividade, distribuição e regulação da fosfofrutoquinase em glândulas salivares de ratos com diabetes induzido por estreptozotocina

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    José Nicolau

    2006-04-01

    Full Text Available Although the influence of diabetes on salivary glands is well studied, it still presents conflicting results. In this work, the regulation of the phosphofructokinase-1 enzyme (PFK-1 was studied utilizing the salivary glands of rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (60 mg/Kg of body weight in rats (180-200 g. The animals were killed 30 days after the induction of diabetes and the submandibular and parotid salivary glands were used. Hyperglycemia was evaluated by blood sugar determination. The distribution of PFK-1 between the soluble and cytoskeleton fractions, the phosphate content of PFK-1, the content of fructose-2,6-bisphosphate and the activity of the PFK-2 enzyme were determined. The calculated relative glandular weight showed a higher value for the parotid gland in comparison with the control, but not for the submandibular gland. The activity of PFK-1 expressed per gland showed no variation between diabetic and control animals. However, considering the specific activity, the soluble enzyme presented a value 50% higher than that of the control and the cytoskeleton bound form increased by 84% compared to the control. For the parotid gland, no difference in the specific activity between diabetic and control animals was observed. On the other hand, the activity per gland of the soluble enzyme increased in the diabetic animals. The phosphate content of PFK-1 increased in the submandibular and parotid glands of diabetic rats. Both the content of fructose-2,6-bisphosphate and the active form of PFK-2 were reduced in the diabetic glands. In conclusion, the increase in the activity of PFK-1 observed in the salivary glands of rats with streptozotocin-induced diabetes does not seem to be due to its modulator fructose-2,6-bisphosphate.Apesar de existirem muitos estudos sobre a influência do diabetes nas glândulas salivares, esses apresentam resultados conflitantes. Neste estudo, a regulação da enzima

  15. Conseqüências no crescimento de maxila e mandíbula de defeito ósseo cirúrgico no ramo da mandíbula de ratos Consequences on the growth of maxilla and mandible of surgical bone defect on mandibular ramus of rats

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    Lucimar Rodrigues

    2004-10-01

    Full Text Available OBJETIVO: Analisar as conseqüências no crescimento de maxila e mandíbula de defeito ósseo cirúrgico simulando fratura no ramo da mandíbula. MÉTODOS: Foram utilizados 25 ratos Wistar com um mês de idade. Sob anestesia geral e por meio de incisão submandibular. Foi realizada osteotomia vertical no ramo da mandíbula do lado direito com emprego de motor cirúrgico. Após período de dois meses os animais foram sacrificados, os tecidos moles retirados e as hemimandíbulas desarticuladas. Foram realizadas incidências radiográficas axiais para o crânio e laterais para as hemimandíbulas. A seguir, por intermédio de um sistema de computador foram obtidas medidas lineares da maxila e das hemimandíbulas. Foi empregado o teste "t" de Student para verificação da significância da diferença entre os lados experimental e controle. RESULTADOS: A diferença foi significante para a altura do ramo (p=0,010 e comprimento da mandíbula referente ao côndilo (p=0,015 e ao ângulo (pPURPOSE: To analyse the consequences on the growth of maxilla and mandible of a surgical bone defect that simulates a mandibular ramus fracture. METHODS: A group of 25 one-month-old Wistar rats were used in this study. Under general anesthesia, and through a submandibular incision, a vertical osteotomy on the right side of the mandibular ramus was made using a surgical drill. The animals were sacrificed after two months, soft tissues removed, and the mandible disarticulated. The skull was submitted to axial radiograph and the hemimandibles to a lateral radiograph. With these, cephalometric mensurations were made through a computer system, and the obtained values submitted to Student's t-test. RESULTS: The height of the mandibular ramus presented significant difference (p=0,010 as well as the length of mandible, both to the condyle (P=0,015 and the angle (p=0,001. There was no significant difference to the mensurations of the maxilla. CONCLUSION: The consequences of the

  16. Efeito da suplementação com L-alanil-L-glutamina sobre a resposta de hipersensibilidade do tipo tardio em ratos submetidos ao treinamento intenso Effect of L-glutamine and L-alanyl-L-glutamine supplementation on the response to delayed-type hypersensitivity test (DTH in rats submitted to intense training

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    Marcelo Macedo Rogero

    2002-12-01

    Full Text Available O treinamento intenso e o exercício exaustivo podem ocasionar imunossupressão em atletas por meio da diminuição da concentração plasmática de glutamina. O presente estudo verificou inicialmente o efeito da suplementação com L-glutamina e L-alanilL-glutamina sobre a resposta ao teste de hipersensibilidade do tipo tardio (HTT em ratos submetidos ao treinamento intenso em natação durante seis semanas. Posteriormente, foi avaliado o efeito dessas intervenções nutricionais sobre a contagem total e porcentual de leucócitos e concentração sérica de anticorpos IgG anti-albumina de soro bovino, em animais submetidos ao teste de exaustão e recuperados durante o período de 3 horas. Não houve efeito do treinamento e da suplementação sobre a resposta ao teste de HTT. Animais suplementados apresentaram maior concentração de glutamina no plasma (PIntense training and exhaustive exercise may cause immunesupression in athletes by reducing plasma glutamine concentration. Initially, this study verified the effect of L-glutamine and L-alanyl-L-glutamine supplementation on the response to delayed-type hypersensitivity test (DTH in rats submitted to intense swimming training for six weeks. Later on, we assessed the effect of these nutritional interventions on total and differential white blood cell counts and on concentration of anti-bovine serum albumin IgG antibodies, in animals submitted to exhaustion test and a three-hour recovery period. There was no effect of training and supplementation on the response to DTH. Supplemented animals presented greatest plasma glutamine concentration (p<0.05, though this increase in glutaminemia did not interfere on the serum IgG antibody concentration. The recovery period after intense exercise resulted in decreased glutaminemia as compared with the values obtained immediately after exhaustion test (p<0.05. Increase in corticosterone levels induced by strenuous exercises led to leukocytosis, neutrophilia

  17. Evaluation of the fuel rod integrity in PWR reactors from the spectrometric analysis of the primary coolant; Avaliacao da integridade de varetas combustiveis em reatores PWR a partir da analise espectrometrica da agua do primario

    Energy Technology Data Exchange (ETDEWEB)

    Monteiro, Iara Arraes

    1999-02-15

    The main objective of this thesis is to provide a better comprehension of the phenomena involved in the transport of fission products, from the fuel rod to the coolant of a PWR reactor. To achieve this purpose, several steps were followed. Firstly, it was presented a description of the fuel elements and the main mechanisms of fuel rod failure, indicating the most important nuclides and their transport mechanisms. Secondly, taking both the kinetic and diffusion models for the transport of fission products as a basis, a simple analytical and semi-empirical model was developed. This model was also based on theoretical considerations and measurements of coolant's activity, according to internationally adopted methodologies. Several factors are considered in the modelling procedures: intrinsic factors to the reactor itself, factors which depend on the reactor's operational mode, isotope characteristic factors, and factors which depend on the type of rod failure. The model was applied for different reactor's operational parameters in the presence of failed rods. The main conclusions drawn from the analysis of the model's output are relative to the variation on the coolant's water activity with the fuel burnup, the linear operation power and the primary purification rate and to the different behaviour of iodine and noble gases. The model was saturated from a certain failure size and showed to be unable to distinguish between a single big fail and many small ones. (author)

  18. Evaluation of the contribution of contamination of radiotherapy room surfaces in the measure of exposure rate of radioiodine therapy patients; Avaliacao da contribuicao da contaminacao de superficies do quarto terapeutico na medida da taxa de exposicao de pacientes de radioiodoterapia

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    Campos, Rafael Ferreira

    2015-07-01

    The contamination of radiotherapy room surfaces is significant and the measures of patient exposure rate are held on the fourth dependencies, relevant questions are raised: the background radiation of the room stay high due to surface contamination, may interfere with the rate of patient exposure at the time of its release? The monitoring site is important to determine whether the patient will be released? The value of the deal activity and the clinical condition of the patient may increase the contamination, influencing the monitoring results? This paper aims to conduct a quantitative analysis of surface contamination of the contribution of therapeutic room at the time is monitored exposure rate from inpatient. Measurements were made regarding the hospitalization of 32 patients with different doses administered activity, age and of both genders. The measurements were performed in the therapeutic rooms at the hospital Brotherhood Santa Casa de Misericordia de Sao Paulo. Exposure rate measurements were performed at the center of the room at 1 meter of the patient on the day of its release. After his release and prior to decontamination, measurements were performed at predetermined landmarks within the therapeutic room. The results revealed that on average background radiation, high due to surface contamination contributes only 2% of the patient dose rate. It can be considered that even with influence of contamination of surfaces, this is insignificant to determine if the patient may or may not be released. This study suggests that the site in which monitoring occurs exposure rate of the patient should not be decisive for liberation thereof. (author)

  19. Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats Avaliação dos efeitos da S-metilisotiouréia, um inibidor da enzima óxido nítrico sintase induzível, na cicatrização de anastomoses colônicas em ratos

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    Romulo Medeiros de Almeida

    2012-12-01

    Full Text Available PURPOSE: To evaluate the effects of S-methylisothiourea hemisulfate (SMT on the healing of colonic anastomosis in rats. METHODS: Sixty rats Wistar were distributed into two groups of 30 animals: experimental (E and control C. The animals of experimental group received intraperitoneal SMT at 50mg/kg/dose every 12 hours for 72 hours. The control group received intraperitoneal saline at the same volume of SMT. The rats were subdivided into subgroups groups of 10 for euthanasia on the third, seventh, and 14th postoperative days (POD. We evaluated clinical and weight evolution, breaking strength and histopathology; also, a blood sample was collected for serum dosage of nitrite/nitrate. RESULTS: There was more vascular neoformation (p=0.006 and granulation (p=0.002 in the E3 group, and more mononuclear infiltrates in the C3 group (p=0.041. There was also more edema in the C14 group (p=0.008. There was no statistically significant difference in breaking strength, nitrite/nitrate dosage, and the remaining histopathological parameters. CONCLUSION: The use of S-methylisothiourea hemisulfate improved the healing of colonic anastomosis in rats on the third postoperative day by accelerating the proliferative stage of healing, but without interfering with the breaking strength of the anastomosis.OBJETIVO: Avaliar os efeitos do hemissulfato de S-metilisotiouréia (SMT na cicatrização de anastomoses colônicas em ratos no terceiro, sétimo e 14° dia de pós-operatório (DPO. MÉTODOS: Sessenta ratos Wistar foram distribuídos em dois grupos: experimental (E e controle (C, com 30 animais cada. No grupo experimental foi administrado SMT 50mg/kg/dose, intraperitoneal a cada 12 horas por 72 horas. O grupo controle recebeu NaCl a 0,9%. Os ratos foram subdivididos em grupos de 10 para eutanásia no terceiro, sétimo e 14° DPO. Avaliou-se a evolução clínica e o peso dos animais, a resistência tênsil e histopatologia da anastomose, e a dosagem de nitrito

  20. Phytochemical study and evaluation of anti-inflammatory activity of Aeschynomene fluminensis Vell. (Fabaceae); Estudo fitoquimico e avaliacao da atividade anti-inflamatoria de Aeschynomene fluminensis Vell. (Fabaceae)

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    Ignoato, Marlene Capelin; Fabrao, Rodrigo Monteiro; Schuquel, Ivania Teresinha Albrecht; Botelho, Marcos Felipe Pinatto; Santin, Silvana Maria de Oliveira [Universidade Estadual de Maringa (UEM) PR (Brazil). Dept. de Quimica; Arruda, Laura Licia Milani de; Bersani-Amado, Ciomar Aparecida [Universidade Estadual de Maringa (UEM) PR (Brazil). Dept. de Farmacologia e Terapeutica; Souza, Maria Conceicao de, E-mail: smoliveira@uem.br [Universidade Estadual de Maringa (UEM) PR (Brazil). Dept. de Biologia

    2012-07-01

    Phytochemical investigation of Aeschynomene fluminensis leaves and branches led to isolation of the flavonoid glycosides kaempferol 3,7-di-O-{alpha}-L-rhamnopyranoside, kaempferol 7-O-{alpha}-L-rhamnopyranoside, kaempferol 3-O-apiofuranosil-7-O- rhamnopyranoside, quercetin 3-O-{alpha}-L-rhamnopyranoside, quercetin 3-O-arabinofuranoside, 8-{beta}-D-glucopyranosyl 4',5,7-trihydroxyflavanone, the isoflavonoid 4',7-di-hydroxy-isoflavone, the dimer epicatechin-(2{beta} ->7, 4{beta} ->8)- epicatechin, the polyol 3-O-methyl-chiro-inositol and two steroids in sitosterol and stigmasterol mixture. These compounds were identified by NMR {sup 1}H and {sup 13}C and compared with literature data. Anti-inflammatory activity of the crude methanolic extract and its fractions was evaluated (author)

  1. Evaluation of pollution effects in transmission lines located in industrial areas; Avaliacao dos efeitos da poluicao em linhas de transmissao situadas em regiao industrial

    Energy Technology Data Exchange (ETDEWEB)

    Kanashiro, Arnaldo G.; Burani, Geraldo F. [Sao Paulo Univ., SP (Brazil). Inst. de Eletrotecnica e Energia; Nascimento, Iara R. [Eletricidade de Sao Paulo SA, SP (Brazil)

    1995-12-31

    This work aims to determinate a correlation between the pollution levels and electric power transmission lines maintenance procedures in order to establish the necessary procedures for specific pollution levels. The methodology is presented. The results obtained in field tests and bench scale tests are presented and discussed. The so called pollution equivalent is determined for each site of the transmission line studied and maintenance procedures are established 6 refs., 5 figs., 4 tabs.

  2. Evaluation of dosimetric effects caused by the table top of therapy; Avaliacao dos efeitos dosimetricos causados pelo tampo da mesa de tratamento

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    Camargo, Andre Vinicius de; Alvares, Bruno; Fioravante, Gustavo Donisete; Silva, Diego da Cunha Silveira Alves da; Giglioli, Milena; Batista, Felipe Placido; Silva, Lais Bueno da; Radicchi, Lucas Augusto [Hospital de Cancer de Barretos, SP (Brazil)

    2016-07-01

    The attenuation and bolus effect for two tables top from different manufacturers were investigated for 6MV photons. The bolus effect of couch was compared with 0,5cm bolus (water equivalent). Maximum attenuation found in Exact Couch table was 6,9% and the minimum was 0,63%. The rail of Exact Couch, for beam in 180 deg, was observed attenuation of 13,61%. The same way that for attenuation, the surface dose was different for each region of couch Exact Couch and for different components of iBeam evo. The percentage of the dose in the depth of 1,8 mm was greater for table top of Exact Couch (66,2%). The extender of table iBeam evo offered increase dose of 38,3% and it table top of 51,9% in the same depth. The bolus increased surface dose in 61,1%. The results of this study showed that table tops when in contact with surface of the patient may significantly increase surface dose and beam attenuation. (author)

  3. Evaluation of quality of tomographs of Rio de Janeiro state, Brazil; Avaliacao da qualidade dos tomografos do estado do Rio de Janeiro

    Energy Technology Data Exchange (ETDEWEB)

    Travassos, P.C.B.; Belem, V.R.; Magalhaes, L.A.; Almeida, C.E.A. de, E-mail: luisalexandregm@hotmail.com [Instituto de Radioprotecao e Dosimetria, (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil). Lab. de Ciencias Radiologicas

    2014-07-01

    Computerized tomographs used in 56 institutions in the state of Rio de Janeiro were evaluated. Criteria of image quality and dosimetry were submitted. The results showed that some not performed optimize their protocols examinations or perform wrongly, which results in an increase in the dose received by patients, and an image in compromised quality. (author)

  4. Assessment of effect of chemical treatment to carnauba's fibers straw; Avaliacao do efeito de tratamento quimico as fibras da palha de carnauba

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, T.M.P. de; Carvalho, L.F.M.; Oliveira, R.R. de; Sousa, F.M.S. de; Sousa, R.C. de; Marques, J.R., E-mail: thaismarjore.pc@gmail.com [Instituto Federal de Educacao, Ciencia e Tecnologia do Piaui (IFPI), Teresina, PI (Brazil)

    2016-07-01

    The use of natural fibers in composite materials has been highlighted in the scientific field. However, its application in polymer matrices usually requires surface modifications. The objective of this work was to treat carnauba's straw fibers with NaOH 1 % and NaOH 5% solutions and measure the water absorption. We used the X-ray diffraction (XRD configuration “Bragg- Brentano) for verification of the crystalline phases and Fourier Transform Infrared Spectroscopy (FTIR) to identify functional groups. The alkali treatment allowed the solubilization of the hemicellulose and lignin without causing changes to cellulose, as indicated by FTIR spectrophotometry and by the increase in crystallinity content. The samples showed the typical peaks of constituents of the fiber. The natural fiber showed an average water absorption of 256 %; fiber treated with NaOH 1%, 315 %; and treated with NaOH 5%, 405 %. Therefore, it is evident improvement in hydrophilicity, fundamental aspect in the interaction fiber / matrix. (author)

  5. Morpho-functional study of ionizing radiation effects on the rabbits` femoral vein; Avaliacao morfofuncional do efeito da radiacao ionizante sobre a veia femoral. Estudo experimental em coelhos

    Energy Technology Data Exchange (ETDEWEB)

    Sakiyama, Mauro Yoshimitsu

    1996-12-31

    In this study we evaluate the effects of the ionizing radiation on the rabbits femoral vein. The samples of femoral vein were obtained from 56 New Zealand rabbits, male with ageing from 90 to 120 days, that were divided into 4 groups of 14 animals: one control group non-irradiated and three animal groups sacrificed 2 days, 14 days and 90 days after irradiation. In the three irradiated rabbits groups, each animal received the total dose 4000 cGy (rads) divided in 10 sessions of 400 cGy, a dose equivalent that utilized on clinical therapeutic. A morpho functional study of vein samples was carried out with: light microscopy: stained by hematoxin - eosin, Masson`s tricromic, and Verhoeff. Immunohistochemical: reactions of immunoperoxidase with monoclonal mouse anti-human endothelial cell factor CD-31 and anti-human Von Willebrand factor (factor VIII), to study the vein endothelium. Histomorphometry of elastic fiber system stained by Weigert`s resorcin-fuchsin with and without prior oxidation with oxone; for the study of mature, elaunin or pre-mature and oxytalan or young elastic fibers. Electronic microscopy: transmission and scanning. With the methodology utilized we observe changes in the femoral vein of the animals submitted to irradiation in relation to the control group, thus described: there is formation of vacuoles between the endothelium and the basal membrane, called sub endothelial vacuoles, in focal areas. The factor VIII and CD-31 endothelial antigens are preserved with no changes in their functions. Focal alterations are present in the endothelial surface with disorder in the setting and orientation of the endothelial cells. there is degeneration of the elastic fibers with significant decrease in their quantity in the stage, 2 days and 14 days after irradiation. There is increase in the quantity of elastic fibers in the late stage, 90 days after irradiation, tending to normality. In this present study, the changes described are not accompanied by venous thrombosis. (author) 101 refs., 22 figs., 2 tabs.

  6. Magnetic resonance imaging pre- and postoperative evaluation of tetralogy of Fallot; Avaliacao pre e pos-operatoria da tetralogia de Fallot por ressonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Bernardes, Renata Junqueira Moll; Simoes, Luiz Carlos [Instituto Nacional de Cardiologia (INC), Rio de Janeiro, RJ (Brazil). Servico de Cardiologia da Crianca e do Adolescente; Marchiori, Edson [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia]. E-mail: edmarchiori@bol.com.br; Bernardes, Paulo Manuel de Barros; Gonzaga, Maria Beatriz Albano Monzo [Rede Labs/D' Or, Rio de Janeiro, RJ (Brazil)

    2004-08-01

    The purpose of this study was to assess the usefulness of magnetic resonance imaging (MRI) in the pre- and postoperative evaluation of patients with tetralogy of Fallot. Twenty patients aged 1 to 29 years were prospectively evaluated with black-blood and contrast-enhanced angiographic techniques, 11 with the classic form of tetralogy of Fallot and 9 with tetralogy of Fallot and pulmonary atresia. MRI studies provided adequate visualization of the aorta that was classified as dilated or not dilated, and definition of its position in all cases. The use of contrast-enhanced MR angiographic techniques provided excellent imaging of the main right and left pulmonary arteries. The results suggest that MRI, including contrast-enhanced angiography techniques, is a useful tool in the evaluation of patients with tetralogy of Fallot before and after cardiac surgery since it provides important anatomical information that is not always obtained with echocardiography. MRI can be considered an alternative to cardiac catheterization, particularly in the evaluation of the pulmonary vascular anatomy. (author)

  7. Evaluation of radon measuring technique using passive detector activated carbon; Avaliacao da tecnica de medicao do radonio utilizando detector passivo com carvao ativado

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Paulo Roberto Rocha; Lessa, Edmilson de Lima; Oliveira, Evaldo Paulo de, E-mail: epoliveira@aluno.ird.gov.br [Instituto de Radioprotecao e Dosimetria, (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil). Div. de Radioprotecao Ambiental e Ocupacional

    2014-07-01

    This study aims to evaluate the efficiency of measuring the radioactive gas Radon ({sup 222} Rn) with passive detector activated carbon. Alpha Guard, exposure chamber, air sampler, default font Radio, calibrator flow, flow adjuster, ducts drivers: For this, various equipment to make the measurement system as were used. An assembly of such equipment, with specific sequence was used allowing for more efficient exposure of passive detectors Radon gas. Twenty samples were heated to remove moisture and then stored in desiccator until the experiment were made. The exhibition was held passive dosimeters being removed from the chamber, and one hour after, subjected to analysis by gamma spectrometry in germanium (HPGe) for an hour. Subsequently, other measurements were made at scheduled times and sequential for one hour. The results were presented in report form and spectra, measures and graphs generated by Alpha Guard were also extracted. Finally we calculated the efficiency of the passive meter activated carbon. (author)

  8. Glass forming ability of the Al-Ce-Ni system; Avaliacao da capacidade de formacao vitrea do sistema Al-Ce-Ni

    Energy Technology Data Exchange (ETDEWEB)

    Triveno Rios, C. [Engenharia Mecanica, Universidade Federal de Mato Grosso, Rondonopolis, MT (Brazil)], e-mail: triveno@ufmt.br; Surinach, S.; Baro, M.D. [Departamento de Engenharia de Materiais - Universidade Federal de Sao Carlos, SP (Brazil); Bolfarini, C.; Botta, W.J.; Kiminami, C.S. [Departamento de Fisica da Universidade Autonoma de Barcelona, Bellaterra (Spain)

    2010-07-01

    In the present work, the glass forming ability (GFA) and its compositional dependence on Al-Ni-Ce system alloys were investigated in function of several thermal parameters. Rapidly quenched Al{sub 85}Ni{sub 15}-{sub X}Ce{sub X} (X=4,5,6,7,10), Al{sub 90}Ni{sub 5}Ce{sub 5}, Al{sub 89}Ni{sub 2}.{sub 4}Ce{sub 8}.{sub 6}, Al{sub 80}Ni{sub 15.6}Ce{sub 4}.{sub 4} and Al{sub 78}Ni{sub 18.5}Ce{sub 3.5} amorphous ribbons were produced by melt-spinning and the structural transformation during heating was studied using a combination of X-ray diffraction (XRD) and differential scanning calorimetry (DSC). The results showed that the GFA and the thermal stability in the Al-rich corner of Al- Ni-Ce system alloys were enhanced by increasing the solute content and specifically the Ce content (author)

  9. Morpho-functional study of ionizing radiation effects on the rabbits` femoral vein; Avaliacao morfofuncional do efeito da radiacao ionizante sobre a veia femoral. Estudo experimental em coelhos

    Energy Technology Data Exchange (ETDEWEB)

    Sakiyama, Mauro Yoshimitsu

    1995-12-31

    In this study we evaluate the effects of the ionizing radiation on the rabbits femoral vein. The samples of femoral vein were obtained from 56 New Zealand rabbits, male with ageing from 90 to 120 days, that were divided into 4 groups of 14 animals: one control group non-irradiated and three animal groups sacrificed 2 days, 14 days and 90 days after irradiation. In the three irradiated rabbits groups, each animal received the total dose 4000 cGy (rads) divided in 10 sessions of 400 cGy, a dose equivalent that utilized on clinical therapeutic. A morpho functional study of vein samples was carried out with: light microscopy: stained by hematoxin - eosin, Masson`s tricromic, and Verhoeff. Immunohistochemical: reactions of immunoperoxidase with monoclonal mouse anti-human endothelial cell factor CD-31 and anti-human Von Willebrand factor (factor VIII), to study the vein endothelium. Histomorphometry of elastic fiber system stained by Weigert`s resorcin-fuchsin with and without prior oxidation with oxone; for the study of mature, elaunin or pre-mature and oxytalan or young elastic fibers. Electronic microscopy: transmission and scanning. With the methodology utilized we observe changes in the femoral vein of the animals submitted to irradiation in relation to the control group, thus described: there is formation of vacuoles between the endothelium and the basal membrane, called sub endothelial vacuoles, in focal areas. The factor VIII and CD-31 endothelial antigens are preserved with no changes in their functions. Focal alterations are present in the endothelial surface with disorder in the setting and orientation of the endothelial cells. there is degeneration of the elastic fibers with significant decrease in their quantity in the stage, 2 days and 14 days after irradiation. There is increase in the quantity of elastic fibers in the late stage, 90 days after irradiation, tending to normality. In this present study, the changes described are not accompanied by venous thrombosis. (author) 101 refs., 22 figs., 2 tabs.

  10. Dynamic system for the evaluation of CIM monitoring and control techniques; Sistema dinamico para avaliacao de tecnicas de monitoracao e controle da CIM

    Energy Technology Data Exchange (ETDEWEB)

    Penna, Monica de Oliveira [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES). Biotecnologia e Ecossistemas]. E-mail: mpena@cenpes.petrobras.com.br; Baptista, Walmar; Brito, Rosane Fernandes de [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES). Tecnologia de Materiais, Equipamentos e Corrosao]. E-mails: walmar@cenpes.petrobras.com.br; rosanef@cenpes.petrobras.com.br; Silva, Edilson Domingos da; Nascimento, Juliana Rangel do [Fundacao Gorceix, Ouro Preto, MG (Brazil)]. E-mails: edilson@cenpes.petrobras.com.br; julianarn@cenpes.petrobras.com.br; Coutinho, Claudia M.L.M. [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil). Inst. de Biologia. Dept. de Biologia Celular e Molecular

    2002-03-01

    In general, the two main problems associated to the water injection system for secondary petroleum recovery are the corrosion control and the decrease in solid injection in the reservoir. Corrosion problems in injection water systems may have abiotic as well as biotic causes, and the occurrence of reservoir plugs can result from the injection of particles (suspended solids as well as corrosion products), bacteria and fouling formation. Due to the need to implement a methodology for the evaluation of abiotic and biotic corrosive processes, monitoring techniques and MIC control in a dynamic system, an apparatus was built that simulates the characteristics of a water injection system. The apparatus allows monitoring and control of the parameters that, when varied, may influence the performance of the quality of the injected water. The dynamic system was designed to simulate as precisely as possible the conditions that allow the SRB and heterotrophic bacteria to create fouling in a water injection system. This article discusses the dynamic system, analytical methods and some preliminary results. (author)

  11. Evaluation of average glandular dose in digital and conventional systems of the mammography; Avaliacao da dose glandular media em sistemas digitais e convencionais de mamografia

    Energy Technology Data Exchange (ETDEWEB)

    Xavier, Aline C.S.; Barros, Vinicius S.M.; Khoury, Hellen J., E-mail: alinecx90@gmail.com, E-mail: vsmdbarros@gmail.com, E-mail: hjkhoury@gmail.com [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear; Mello, Francisca A. de, E-mail: francissamello@yahoo.com.br [Hospital das Clinicas do Recife (HCR/UFPE), PE (Brazil)

    2014-07-01

    Mammography is currently the most effective method of diagnosis and detection of breast pathologies. The main interest in this kid of exam comes from the high incidence rate of breast cancer and necessity of high quality images for accurate diagnosis. Digital mammography systems have several advantages compared to conventional systems, however the use of digital imaging systems is not always integrated to an image acquisition protocol. Therefore, it is questionable if digital systems truly reduce the dose received by the patient, because many times is introduced in the clinics without optimization of the image acquisition protocols. The aim of this study is to estimate the value of incident air Kerma and average glandular dose (AGD) in patients undergoing conventional and digital mammography systems in Recife. This study was conducted with 650 patients in three hospitals. The value of incident air Kerma was estimated from the measurement of the yield of equipment and irradiation parameters used for each patient. From these results and using the methodology proposed by Dance et al. the value of the average glandular dose was calculated. The results obtained show that the lowest value of AGD was found with conventional screen-film system, indicating that the parameters for image acquisition with digital systems are not optimized. It was also observed that the institutions with digital systems use lower breast compression values than the conventional. (author)

  12. Assessment pozzolanicity waste red ceramics produced in Valley Assu / RN; Avaliacao da atividade pozolanica dos residuos de ceramica vermelha produzidos no Vale do Assu / RN

    Energy Technology Data Exchange (ETDEWEB)

    Palhares, Rodolfo de Azevedo; Pereira, Arthur Ruan da Silva; Cabral, Kleber Cavalcanti; Nobrega, Andreza Kelly Costa, E-mail: rodolfo.palhares@hotmail.com, E-mail: arthurruan_rn@hotmail.com, E-mail: kleber.cabral@ufersa.edu.br, E-mail: akcn123@hotmail.com [Universidade Federal Rural do Semi-Arido (UFERSA), Mossoro, RN (Brazil). Departamento de Ciencias Exatas

    2016-07-01

    It is known that both the cement industry as a ceramist contribute much to the generation of environmental impacts. Be the Co2 in the atmosphere, as well as the generation of excessive waste, reaching 20%. The objective of this study is to analyze the potential pozollanic of waste from the red ceramic industries Valley Assu / RN, in order that this material can be incorporated as alternative raw material in the manufacture of ecological and similar brick, replacing partially in its composition Portland cement. Thus contributing to reducing the environmental impact produced by both the ceramics industry, such as cement. To evaluate the efficiency of pozollanic material, it was made sample preparation and then the physico-chemical characterization. After performing tests, it was noticed that the material has the minimum requirements established in standard to be considered as pozollanic material. (author)

  13. Falling hammer use evaluation for hot deformation hardness testing; Avaliacao do uso de um martelo de queda, para a determinacao da resistencia a deformacao a quente

    Energy Technology Data Exchange (ETDEWEB)

    Beck, J.C.P.C.; Cauduro, Carlos R.; Schaeffer, Lirio [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil)

    1990-12-31

    This work performs a evaluation of the characteristics hot leaking of a 8620 stainless steel, deformed at 870 deg C. The tools associated with the measurements was described and a comparison between the theoretical values and the values experimentally obtained from the performed tests. 5 figs., 3 refs.

  14. Magnetic resonance imaging of sella turcica: evaluation of patients with galactorrhea, amenorrhea and hyperprolactinemia; Ressonancia magnetica da sela turca: avaliacao de pacientes com galactorreia, amenorreia e hiperprolactinemia

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Alair Augusto Sarmet M.D. dos [Hospital Universitario Antonio Pedro, Niteroi, RJ (Brazil). Dept. de Radiologia; Moreira, Denise Madeira [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia; Andreiuolo, Pedro Angelo [Beneficencia Portuguesa de Niteroi, RJ (Brazil). Servico de Radiologia Santa Cruz Scan

    1999-08-01

    We have selected 135 cases of patients who have done magnetic resonance imaging of sella region, carried out from September, 1991 to August, 1996, who had galactorrhea (G), amenorrhea(A), and hyperprolactinemia (H), isolated or in association. The patients were divided in seven groups, according to the presence of these symptoms and signs. All examinations were made in a private clinic in the city of Rio de Janeiro, Brazil. Correlating these patients with the results of the magnetic resonance images, we found 57 micro adenomas, 31 normal examinations, 22 macro adenomas, 11 pituitary hyperplasias, 7 empty sella and 7 cases included in other aspects. The micro adenoma predominated in groups 1 (GAH), 2 (GH), 3 (HA) and 5 (H), that is, in all groups whose patients had hyperprolactinemia. In macro adenomas, a bright signal on T 1-weighted images indicates pituitary apoplexy with intratumoral hemorrhage. All in all, the magnetic resonance imaging is excellent method to be used in the evaluation of patients with changes in the hypothalamic-pituitary axis. (author)

  15. Evaluation of occupational exposure during the exploration and physical processing of monazite; Avaliacao das exposicoes ocupacionais durante as etapas de exploracao e beneficiamento fisico da monazita

    Energy Technology Data Exchange (ETDEWEB)

    Farias, Joao Torres de

    2006-07-01

    In Brazil the most important concentrations of monazite sands occur in the beaches and sandbanks that extend throughout the coast, from the State of Rio Grande do Norte down to the State of Rio Grande do Sul, Brazil. Nowadays, the most important Brazilian monazite concentrations - considered as being the fourth worldwide reservoir of rare-earth elements - are located at the north seacoast of the State of Rio de Janeiro, near Buena town. At this location the Brazilian Nuclear Industries - INB - manages an industrial complex for prospecting, mining and physical separation and concentration of monazite sands. Among the elements present at the monazite sands there are the radioelements thorium and uranium, which make the monazite sand a radioactive mineral. Monazite is constituted basically of 62% in TR{sub 2}0{sub 3} (rare-earth oxides), 5,5% of thorium in Th0{sub 2} (thorium oxide) and 0,25% of uranium in U{sub 3}0{sub 8} (uranium oxide). The general objective of the present work was to estimate the occupational doses at the industrial complex for prospecting, mining and physical separation and concentration of monazite sands located at Buena, at the north of the State of Rio de Janeiro. The occupational exposures occur mainly through the inhalation of airbone dust particles of mineraIs containing thorium and uranium, during the prospection and physical separation and concentration of monazite. To evaluate the impact of the occupational exposures during the physical separation and concentration of monazite, it was necessary to evaluate the effective equivalent doses due to thorium contamination of the air and the exposures due to the daughter radionuclides from the natural series of uranium and thorium. The results obtained at the present work showed that, although occupational doses are within the annual dose limits established by the Brazilian regulatory authority - CNEN - in some of the working places within the industrial complex the concentrations of radionuclides in air are above the maximum recommended levels. (author)

  16. Synthesis and preliminary evaluation of N-acylhydrazone compounds as antibacterial and antifungal agents; Sintese e avaliacao preliminar da atividade antibacteriana e antifungica de derivados N-acilidrazonicos

    Energy Technology Data Exchange (ETDEWEB)

    Cachiba, Thomas Haruo; Carvalho, Bruno Demartini; Carvalho, Diogo Teixeira [Universidade Federal de Alfenas, MG (Brazil). Fac. de Ciencias Farmaceuticas. Dept. de Alimentos e Medicamentos; Cusinato, Marina; Prado, Clara Gaviao; Dias, Amanda Latercia Tranches, E-mail: diogo.carvalho@unifal-mg.edu.br [Universidade Federal de Alfenas, MG (Brazil). Inst. de Ciencias Biomedicas

    2012-07-01

    We describe the synthesis and evaluation of N-acylhydrazone compounds bearing different electron-donating groups in one of its aromatic rings, obtained using a four-step synthetic route. IC{sub 50} values against pathogenic fungi and bacteria were determined by serial microdilution. Compounds showed low activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. By contrast, a derivative with a meta-oriented electron-donating group showed significant activity (IC50) against Candida albicans (17 {mu}M), C. krusei (34 {mu}M) and C. tropicalis (17 {mu}M). Results suggest this is a promising lead-compound for synthesis of potent antifungal agents. (author)

  17. Assessment of activity of nitrate-reducing bacterial souring control; Avaliacao da atividade de bacterias redutoras de nitrato no controle de acidificacao de reservatorios de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Sousa, Kally A. de; Cammarota, Magali C.; Servulo, Eliana F.C. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Escola de Quimica

    2008-07-01

    The effect of nitrate addition in relation to NRB concentration was evaluated on biogenic H{sub 2}S generation in anaerobic microcosms with produced water. A 2{sup k} factorial experimental design was performed by using as response variables nitrate (NO{sub 3}{sup -}) and sulfate (SO{sub 4}{sup =}) consumption and nitrite (NO{sub 2}{sup -}) and sulfide (H{sub 2}S) production and as independent variables the SRB and NRB (10{sup 1} - 10{sup 7} MPN/mL) and NO{sub 3}{sup -} (127.5 - 727.5 mg/L) initial concentrations. Each condition was carried out under incubation at 30 deg C during 7, 14 and 28 days. The lowest sulfide production (0.4 - 0.8 mg/L) was achieved for 10{sup 4} MPN/mL of SRB and NRB and 427.5 mg/L nitrate. Also, a reduction of sulfide generation was obtained by nitrate addition when low SRB and NRB concentrations were established. In such condition, the produced sulfide is rather dependent of nitrate concentration. The increase of the NRB concentration has not resulted in the reduction of sulfide production, even when higher nitrate concentrations were used. (author)

  18. Assessment in dogs tympanic bulla, through virtual tomographic endoscopy; Avaliacao de bulas timpanicas em caes, por meio da endoscopia tomografica virtual

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Luciana Carandina da; Sabino, Emanuelle Guidugli, E-mail: lucianacarandina@uol.com.br [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Faculdade de Medicina Veterinaria. Dept. de Reproducao Animal e Radiologia Veterinaria; Vulcano, Luiz Carlos; Machado, Vania Maria de Vasconcelos [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Faculdade de Medicina Veterinaria

    2012-07-01

    Dogs usually have problems related to the auditory canal. For the diagnosis of these pathologies, it is necessary a physical examination and, in some cases radiographic examination and computed tomography. The tympanic bulla is not easily visualized radiographically, since there is many structures of the brain overlaying the image obtained. The computed tomography has been the technique of choice to assess this structure faithfully. A new alternative assessment of the tympanic bulla is tomographic virtual endoscopy, which allows an improvement of the image obtained through the virtual tomographic technique. This paper provides information on the use of computed tomography, and a new technique, tomographic virtual endoscopy, in order to make the improvement of these techniques, and prove the reliability of these changes in the diagnosis of ear canals of dogs. Therefore, we performed the computed tomography of the tympanic bulla on healthy animals, and later performed image reconstruction in three-dimensional (3D) mode for virtual endoscopy. (author)

  19. Photoluminescence evaluation of TiO{sub 2} synthesized by Pechini method; Avaliacao da fotoluminescencia do TiO{sub 2} sintetizado pelo metodo Pechini

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, D.S.; Diniz, V.C.S.; Dantas, J.; Araujo, P.M.A.G.; Costa, A.C.F.M., E-mail: dagobertoufcg@gmail.com [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Programa de Pos-Graduacao em Ciencia e Engenharia de Materiais; Viana, R.S.; Junior, S.A. [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. Quimica Fundamental; Torquato, R.A. [Universidade Federal da Paraiba (UFPB), Joao Pessoa, PB (Brazil). Dept. de Engenharia de Materiais

    2017-07-15

    Titanium dioxide (TiO{sub 2}) stands out for use in various applications mainly due to its properties of thermal and chemical stability and its excellent optical properties. However, these properties are dependent on the type and phase morphology, which is related to the TiO{sub 2} processing method. Thus, this paper proposed to synthesize TiO{sub 2} nanoparticles by polymeric precursor method with different polymorphs, and evaluate the influence of these phases in the photoluminescent properties. For this, the stoichiometric molar proportions of citric acid:metallic cations of 1:1, 2:1, 3:1, 4:1 and 5:1 were investigated. The nanoparticles were characterized by X-ray diffraction (XRD), infrared spectroscopy, textural analysis, scanning electron microscopy (SEM), excitation and emission spectroscopy and determination of the band gap by UV-Vis diffuse reflectance spectroscopy. The presence of anatase and rutile was confirmed by XRD in different proportions for each sample. The agglomerate size increased with the citric acid/metal cations ratio as observed by SEM. Concerning photoluminescence, the maximum intensity in the emission spectrum occurred at the wavelength of 533 nm for the 3:1 sample and the maximum intensity in the excitation spectrum occurred for the 2:1 sample at the wavelength of 451 nm. (author)

  20. Radiographic and tomographic study of the elbow joint in dogs; Avaliacao morfologica e morfometrica da articulacao umerorradioulnar em caes atraves de exames radiograficos e por tomografia computadorizada

    Energy Technology Data Exchange (ETDEWEB)

    Sendyk-Grunkraut, Alessandra; Martin, Claudia M.; Souza, Alexandre N.A.; Patricio, Geni Cristina F.; Lorigados, Carla A.B.; Matera, Julia M.; Fonseca-Pinto, Ana C.B.C., E-mail: anacarol@usp.br [Universidade de Sao Paulo (FMVZ/USP), SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia

    2017-02-15

    Elbow dysplasia disease includes an united anconeal process, fragmented medial coronoid process, osteochondrosis of humeral trochlea, articular incongruity and degenerative joint disease. The aim of this study was to present detailed morphologic and morphometric aspects of the elbow joint in dog in clinical and correlate with radiographic and tomographic (CT) exam. Inter-observer variation for articular incongruity measurements by CT, comparative analysis in the radiographic exam, angle in ulnar notch and its comparative analysis between radiographic and tomographic agreement examination in 44 elbow of dogs with different ages were evaluated. The statistics analyses included the kappa coefficient and interclass correlation and Fischer's test and McNemar's test. It was evidenced that individual performance of each radiographic incidence had poor agreement with the tomographic exam, suggesting that the accomplishment of more than two radiograph views are needed. There was no agreement between the three evaluators in the ulnar notch angle at radiographic and tomographic exams. However, there was good/moderate agreement for articular incongruity measurement in the sagittal plane between evaluators. It was possible to conclude that none of the five radiographic incidences was better than the others for radiographic analysis because each incidence had a better identification of a particular elbow compartment; measurements at the tomographic exam to evaluate radioulnar incongruity had no reproductiveness in the frontal plane, but in sagittal plan had a good/moderate agreement between observers and the angle in ulnar notch presented no repeatability at radiographic exam and no reproductiveness at tomographic exam. (author)

  1. Evaluation of impact strength of polyamide 6/bentonite clay nanocomposites; Avaliacao da resistencia mecanica sob impacto de nanocompositos de poliamida 6/argila bentonitica

    Energy Technology Data Exchange (ETDEWEB)

    Paz, Rene A.; Leite, Amanda M.D.; Medeirosa, Vanessa da N., E-mail: rene@cct.ufcg.edu.b [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Curso de Pos-Graduacao em Ciencia e Engenharia de Materiais; Araujo, Edcleide M.; Melo, Tomas J.A. [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Dept. de Engenharia de Materiais; Pessan, Luiz A. [Universidade Federal de Sao Carlos (DEMa/UFSCAR), SP (Brazil). Dept. de Engenharia de Materiais

    2010-07-01

    Nanocomposites of polymer/clay have had much attention in recent years, particularly those developed with layered silicates due to the need of engineering materials more efficient than pure polymers for certain applications. The level of exfoliation of layered silicates in crystalline structure of polymer matrices has been studied and has been observed that they affect the crystalline behavior and the physical and mechanical properties. In this study, nanocomposites of polyamide 6 were obtained by the melt intercalation method, using a regional bentonite modified with a quaternary ammonium salt in an amount of 3% by weight. XRD results showed that incorporation of salt among the layers of clay, making it organophilic and obtaining exfoliated and/or partially exfoliated structures. The impact properties of the nanocomposites showed inferior in relation to pure polyamide, in other words, lost of toughness. (author)

  2. Assessment and epidemiology of Chagas' disease in patients treated in Araguaina - Tocantins; Avaliacao e epidemiologia da cardiopatia chagasica em pacientes atendidos em Araguaina - Tocantins

    Energy Technology Data Exchange (ETDEWEB)

    Correa, Valeria Rita

    2010-07-01

    Chagas disease (AD) was described by Carlos Chagas in 1909. It is caused by a parasite T. cruzi, transmitted by bugs, by blood transfusion, vertical and orally. The DC has two phases: acute and chronic. The evolution to the cardiac form occurs in about 30% of chronic cases and is the largest cause of mortality in chronic Chagas disease. The aim of this study was to Chagas' disease in patients of Tocantins, compared with other heart patients and asymptomatic from the standpoint of non-invasive exams using radiant energies such as echocardiography and ECG and RX. The descriptive study included 80 patients, 20 chronic form of Chagas disease, 20 indeterminate, 20 with other heart diseases, and 20 controls. There was a prevalence of 9.5% of chagasic patients treated in outpatient cardiology at Araguaina Tocantins, and 7.3% in chronic and 2.21% in the indeterminate. Of the chronic patients in the study 50% had mega esophagus and megacolon 4 (20%). Most patients had no family history of AD, nor was a smoker or drinker. Major electrocardiographic abnormalities found refer to driving. The evaluation of ICT, the chronic chagasic showed that increased by 40% of patients, 40% had esophageal changes and 20% of patients had megacolon s. The echocardiogram was abnormal in 42%). 27% of patients had EF below 55% changed. Changes in segmental contractility and Asynchrony septum were found in 80% of chronic Chagas disease. In 80% of the patients was observed diastolic dysfunction. The valvular changes occurred in 75%. Electrocardiographic abnormalities occurred in 80% of patients with CCC, while the other heart had ECG changes. Arterial hypertension had an incidence of 45% in patients with CCC and 40% in FCI. The systolic and diastolic ventricular dysfunction was more prevalent in groups that had an abnormal ECG and arrhythmia. Observed that the group of chagasic decreased ejection fraction is correlated to a higher incidence of arrhythmias besides diastolic dysfunction and related increased atrio-left, ICT correlates with LV size and EF and LA. The abnormal ECG also are related to the dysfunction. (author)

  3. Assessment of natural radioactivity in wall paints of commercial use in Brazil; Avaliacao da radioatividade natural em tintas de uso comercial no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Fonseca, Leandro Milhomens da

    2016-11-01

    Natural radioactivity in soils, rocks and construction materials, due to {sup 40}K and the natural series of {sup 232}Th and {sup 2}'3{sup 8}U, is the main contribution to external exposure in mankind. In this work, activity concentrations of {sup 226}Ra ({sup 238}U series), {sup 232}Th and {sup 4}'0K were determined for 50 white latex wall paints samples, commercialized in Brazil, namely 15 Economic quality samples, 15 Standard quality samples and 20 Premium quality samples and for a single titanium dioxide sample. The samples were tightly sealed and stored for a minimum period of 30 days, to reach the radioactive secular equilibrium from {sup 238}U and {sup 232}Th series, then measured by high resolution gamma ray spectrometry. The activity concentration was determined using the weighted average concentrations of {sup 214}Pb and {sup 214}Bi for {sup 226}Ra and {sup 228}Ac, {sup 212}Pb and {sup 212}Bi for {sup 232}Th. The {sup 4}'0K activity concentration was determined by its single transition of 1460.8 keV. Self attenuation correction factors of the samples whose densities are higher than 1.0 g.cm{sup -3}, were determined and used to make the necessary corrections. The radiological indices radium equivalent activity (Ra{sub eq}), activity concentration index (I{sub γ}), internal exposure risk index (H{sub in}) and external exposure risk index (H{sub ex}) and also the absorbed dose rate (D) and annual effective dose (D{sub ef}) were calculated from the activity concentrations of {sup 226}Ra, {sup 232}Th and {sup 40}K. The activity concentration values for {sup 226}Ra ranged from under the minimum detectable activity to 38.7 Bq.kg{sup -1}, for {sup 232}Th from under the minimum detectable activity to 101.2 Bq.kg{sup -1} and for {sup 40}K from under the minimum detectable activity to 256 Bq.kg{sup -1}. Ra{sub eq} ranged from 1.41 Bq.kg{sup -1} to 203 Bq.kg{sup -1}, I{sub γ} ranged from 0.0047 to 0.720, H{sub in} from 0.0076 to 0.653 and H{sub ex} from 0.0038 to 0.549. The absorbed dose rate ranged from 0.170 nGy.h{sup -1} and 21.3 nGy.h{sup -1} and the annual effective dose ranged from 0.83 μSv and 104.2 μSv. The results show that the activity concentrations of the wall paints studied in this work are below the recommended limits by Hassan et al. for Ra{sub eq} (370 Bq.kg{sup -1}), by European Commission for I{sub γ} (limit of 2 for superficial materials) and by the Organisation for Economic Co-operation and Development for H{sub in} and H{sub ex} (both with limit of 1), for all the 50 samples, assuring the safety of these wall paints with respect to radiological protection. (author)

  4. Renewability emergy index calculation in the evaluation of the sustainability of a national economy; Calculo do indice de renovabillidade emergetica na avaliacao da sustentabilidade de uma economia nacional

    Energy Technology Data Exchange (ETDEWEB)

    Siche Jara, Raul Benito [Universidad Nacional de Trujillo, La Libertad (Peru). Fac. de Ciencias Agropecuarias. Escuela de Ingenieria Agroindustrial], e-mail: Siche.J.R@gmail.com; Ortega Rodriguez, Enrique [Universidade Estadual de Campinas (DEA/FEA/UNICAMP), SP (Brazil). Lab. de Engenharia Ecologica e Informatica Aplicada], e-mail: ortega@fea.unicamp.br

    2006-07-01

    In this study, the emergy methodology was used to analyze the sustainability of the Peruvian economic system. The resources (natural and not natural) and importations had been accounting in units of solar emergy using data of the Peruvian economy for the year 2004. Emergy is an energy measure based in the contribution of the resources and its influence, defined as the energy of a type required producing a flow or storage of another type. The focus of this study is the calculation of the emergy index call 'renewability' (REN), considered as a general measure of the ecological sustainability. In a long period, only systems or processes with high REN are sustainable. This index is calculated by the accounting of the resources renewed used in the economy in emergy units (2.17E+23 seJ) and divided by emergy total that enters to the economic system (6.93E+23 seJ), resulting a REN of 0.31. The renewable resources that use Peru almost represent 20% of the total of renewable resources available in the system. The great amount of renewable resources that Peru can potentially use was calculated in 11.44E+23 seJ, meaning that the system can be more sustainable if the economy is based on increasing the use of renewable resources and to diminish the use of non-renewable resources and imported resources. These data show that Peru has a relatively sustainable economy that can improve or get worse, depending of its politics in the use of resources. (author)

  5. Evaluation of effects of ionizing radiation on materials used in dental restorations;Avaliacao dos efeitos da radiacao ionizante em materiais utilizados em restauracoes dentarias

    Energy Technology Data Exchange (ETDEWEB)

    Maio, Mireia Florencio

    2009-07-01

    This work consisted of quantitative studies of the effects caused by ionizing radiation on materials used in dental restorations (Titanium, Amalgam, Resin Composite and Glass Ionomer) aiming the deleterious effects of radiotherapy when patients with tumors in head and neck, arising when the teeth are restored within in the field of radiation. Samples were submitted to X-ray beams of 6 MV from a linear accelerator, VARIAN 2100C model. The samples were analyzed by X-ray fluorescence techniques to compare the chemical composition before and after the irradiation. The sample were submitted to Geiger-Mueller detectors and the ionization chambers in order to verify any residual radiation in the samples. The samples were also analyzed by gamma spectrometry by a Germanium detector. These tests were performed to determine small changes in the composition in the samples due to the radiation interaction. The results of this study may encourage the development of new research for alternative materials in dental restorations that can contribute to improve the quality of life of those patients with tumors of the mouth. (author)

  6. Synthesis optimization of calcium aluminate cement phases for biomedical applications; Avaliacao da sintese das fases de cimento de aluminato de calcio

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, T.L.; Santos, G.L.; Oliveira, I.R. [Universidade do Vale do Paraiba (UNIVAP), Sao Jose dos Campos, SP (Brazil); Pandolfelli, V.C. [Universidade Federal de Sao Carlos (UFSCar), SP (Brazil)

    2011-07-01

    Calcium aluminate cement (CAC) has been studied as a potential material for applications in the areas of health such as, endodontics and bone reconstruction. These studies have been based on commercial products consisting of a mixture of phases. Improvements can be attained by investigating the synthesis routes of CAC aiming the proper balance between the phases and the control of impurities that may impair its performance for biomedical applications. Thus, the aim of this work was to study the CAC synthesis routes in the Al{sub 2}O{sub 3}-CaCO{sub 3} and Al{sub 2}O{sub 3}-CaO systems, as well as the phase characterization attained by means of X ray analysis. The Al{sub 2}O{sub 3}-CaO route enabled the production of the target phases (CA, CA{sub 2}, C{sub 3}A and C{sub 12}A{sub 7}) with a higher purity compared to the Al2O3-CaCO3 one. As a result the particular properties of these phases can be evaluated to define a more suitable composition that results in better properties for an endodontic cement and other applications. (author)

  7. Sintering evaluation of spinel MA nanostructured obtained via synthesis of combustion in solution; Avaliacao da sinterizacao de espinelio MA nanoestruturado obtido via combustao em solucao

    Energy Technology Data Exchange (ETDEWEB)

    Vitor, P.A.M.; Braganca, S.R.; Bergmann, C.P., E-mail: pedroaugusto89@hotmail.com [Universidade Federal do Rio Grande do Sul (LACER/UFRGS), Porto Alegre, RS (Brazil). Laboratorio de Materiais Ceramicos

    2016-07-01

    Specimens were shaped from powders of spinel MA (MgAl2O4) obtained via synthesis combustion in solution (SCS) from the trio chemical precursor aluminum nitrate, magnesium nitrate and sucrose in water, and then sintered at different temperatures between 1350 and 1650 ° C. Were evaluated the physical properties (density, porosity, water absorption and linear shrinkage), mechanical (flexural 4 points), and the microstructures (SEM) as a function of sintering temperature. The densification increased with growing the value of the sintering temperature, wherein the temperature to 1650 ° C had the highest flexural strength and higher elastic modulus: 51.33 (± 6.83) and 26.16 MPa (± 5.06) GPa respectively. The study confirmed the microstructure of the densification of the particles, confirming the purity and nature of nanometric powders obtained via the SCS. (author)

  8. Preliminary results of the evaluation of biomass use as energy sources; Resultados preliminares da avaliacao do uso de biomassas como fontes de energia

    Energy Technology Data Exchange (ETDEWEB)

    Rocha Ferreira, Leo da; Tourinho, Octavio A.F. [Instituto de Planejamento Economico e Social (IPES), Rio de Janeiro, RJ (Brazil). Inst. de Pesquisas

    1985-12-31

    This article discusses the preliminary results of the BIOMASSA model of IPEA with emphasis on three aspects: temporal and spatial crop mix patterns along the analysis horizon; evaluation of the social cost of alcohol fuel ; and the impact of alcohol production on the balance of payments. It concludes that the PROALCOOL increases domestic costs, but the impact on the balance of payments is positive and substantial. (author). 8 tabs

  9. Evaluation of the efficiency of injection of polyacrylamide in different reservoir-rock samples; Avaliacao da eficiencia de injecao de poliacrilamida em diferentes amostras de rocha-reservatorio

    Energy Technology Data Exchange (ETDEWEB)

    Marcelino, Cleuton P.; Valentim, Adriano C.M.; Medeiros, Ana Catarina R. de; Girao, Joaquim H.S.; Barcia, Rosangela B. [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil)

    2004-07-01

    Water soluble polymers have been used extensively in the petroleum recovery, due to their ability in increasing the viscosity of the injection water and to reduce water/oil mobility ratio and the water relative permeability in the reservoir. This reduction acts favorably as a secondary effect, and it reestablishes part of the pressure in the reservoir after the flow of the polymer, causing a correction of the injection profile in the wells through the restructuring of the resident fluids in the porous media. Nevertheless, some parameters influence the improve of this mechanism, such as petrophysics properties, chemical composition of the rock, adsorption, resistance factor and the residual resistance factor. Many paper in the area of polymers applied to the enhanced petroleum recovery indicate a high efficiency in the injection of different partially hydrolysed polyacrylamides, in different concentrations, or even in different injection conditions, as: temperature, flow, among others. In this work it was evaluated the behavior and efficiency of partially hydrolysed polyacrylamide flooding on outcrop cores from Botucatu, Rio Bonito, Clashach and Assu, using core flow tests and computer simulations. (author)

  10. Characterization and evaluation of residue 'grits' of the cellulose industry; Caracterizacao e avaliacao do resisduo 'grits' da industria de celulose

    Energy Technology Data Exchange (ETDEWEB)

    Destefani, A.Z.; Santos, M.M.; Holanda, J.N.F. [Universidade Estadual do Norte Fluminense Darcy Ribeiro (LAMAV/CCT/UENF), Campos dos Goytacazes, RJ (Brazil). Lab. de Materiais Avancados

    2010-07-01

    The cellulose industry generates huge amounts of solid waste residue called 'grits'. These wastes have been willing over time in landfills near the mills. However, this type of disposal is not environmentally friendly and can cause degradation and environmental pollution. In addition, environmental legislation increasingly severe and the high costs of landfill have led the search for new alternatives for final disposition of this abundant waste. In this context, this study is to characterize waste grits, generated by the cellulose industry in the region of Aracruz-ES. The residue samples were characterized in terms of chemical composition, X-ray diffraction, particle size distribution and thermal analysis (DTA and TGA). The characterization of the residual 'grits' demonstrated its potential as a feedstock for production of soil-cement bricks. (author)

  11. Evaluation of bioactivity in vitro of endodontic calcium aluminate cement; Avaliacao da bioatividade in vitro de cimento endodontico a base de aluminato de calcio

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, I.R.; Andrade, T.L.; Santos, G.L., E-mail: ivonero@univap.br [Universidade do Vale do Paraiba (UNIVAP), Sao Jose dos Campos, SP (Brazil); Pandolfelli, V.C. [Universidade Federal de Sao Carlos (UFSCar), SP (Brazil)

    2011-07-01

    Bioactivity is referred to as the capacity of a material to develop a stable bond with living tissue via the deposition of hydroxyapatite. Materials which exhibit this property can be used to repair diseased or damaged bone tissue and can be designed to remain in situ indefinitely. An indication of bioactivity can be obtained by the formation of a hydroxyapatite layer on the surface of a substrate in simulated body fluids (SBF) in vitro. Therefore, set samples of calcium aluminate endodontic cement were maintained in contact with SBF solutions (Kokubo and Rigo) and their surfaces were later evaluated by means of SEM, EDX and DRX. Measurements of pH and ionic conductivity were also carried out for SBF solutions in contact with set samples of endodontic cement. The ideal conditions of precipitation were obtained in SBF Rigo been observed a surface layer with spherical morphology characteristic of stoichiometric hydroxyapatite.(author)

  12. Evaluation of the inflammatory activity in chronic osteomyelitis. Contribution of the scintigraphy with polyclonal antibodies; Avaliacao de atividade inflamatoria em osteomielite cronica. Contribuicao da cintilografia com anticorpos policlonais

    Energy Technology Data Exchange (ETDEWEB)

    Sapienza, Marcelo Tatit

    1996-07-01

    Active chronic osteomyelitis or complicating osteomyelitis (superimposed on diseases that changes the normal bone structure fractures, post-surgery, prosthesis) can be difficult to diagnose by anatomic radiological imaging modalities, like plain radiograph and CT. These diseases frequently cause also increased bone remodeling, leading to nonspecific uptake of Tc-99m-bone scan agents and gallium-67. New radiopharmaceuticals with greater inflammation/infection avidity and specificity are being developed, including the nonspecific polyclonal immunoglobulin (IgG) labeled with technetium-99. Tc-99m-IgG may be available as a ready to use kit, with no reported side effects, low patient absorbed radiation dose and low cost. The mechanism of IgG uptake at the inflammation site has not been fully elucidated yet. Specific (receptor linking, physico-chemical immunoglobulin properties) and nonspecific mechanisms (enhanced vascular permeability and macromolecular exudate) has been suggested. IgG scintigraphy results are affected by the isotope, labeling procedure adopted and characteristics of the inflammatory focus. Nineteen patients with suspected osteomyelitis (active chronic osteomyelitis or violated bone osteomyelitis) were studied by Tc-99m-IgG scintigraphy (directly labeled polyclonal immunoglobulin, Sandoglobuilina - Sandoz). All patients also underwent standard three-phase bone scintigraphy using methylene diphosphonate (Tc-99m-MDP), gallium-67 scintigraphy and plain radiographs. Infection was found in 8 sites. Sensitivity and specificity for Tc-99m-MDP, gallium-67 and Tc 99m-IgG scintigraphy were, respectively, 88 and 36%, 75 and 73%,88 and 82%. All patients with false positive IgG scintigraphies had previous surgery. Other current scintigraphic procedures used in the diagnosis of osteomyelitis are also reviewed. (author)

  13. Evaluation of maximum absorbed dose for accompanying patients in nuclear medicine establishments; Avaliacao da dose maxima absorvida por acompanhantes de pacientes em servicos de medicina nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Cabral, Geovanna; Amaral, Ademir; Hazin, Clivis A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear; Lima, Ricardo A.; Nogueira, Maria S. [Centro Regional de Ciencias Nucleares (CRCN), Recife, PE (Brazil); Lopes, Ferdinand [Centro de Medicina Nuclear de Pernambuco (CEMUPE), Recife, PE (Brazil); Guimaraes, Maria Ines C.C. [Sao Paulo Univ., SP (Brazil). Centro de Medicina Nuclear

    2001-07-01

    In nuclear medicine, radioisotopes are bound to various pharmaceuticals for use in diagnostic and therapeutic applications. These unsealed sources are administered in various forms to patients, who remain radioactive for hours or days, and represent a source of potential radiation exposure for others. Thus, in nuclear medicine departments, radiation protection of workers and members of the public (especially persons accompanying patients) must consider this exposure. In this study, thermoluminescent dosimeters were given to various persons who were accompanying patients in two nuclear medicine departments in Recife, Pernambuco State, Brazil. Exposure results are given, and issues regarding exposure conditions and times for members of the public in these departments are discussed. (author)

  14. Powder addition assessment of manganese residue ceramic matrix coating; Avaliacao da adicao do po de residuo de manganes em matriz ceramica para revestimento

    Energy Technology Data Exchange (ETDEWEB)

    Conceicao, A.C.R. da; Santos, O.C.; Leao, M.A., E-mail: arangel-ma@hotmail.com [Instituto Federal de Educacao, Ciencia e Tecnologia da Bahia (IFBA), BA (Brazil)

    2016-07-01

    The use of recycled materials in the composition of new products follows the production's worldwide trending, meeting new technological requirements and environmental concerns. This work aims to utilize the residue of manganese dust on ceramic mass for the production of ceramic coating. The raw materials were characterized by both x-ray fluorescence and diffraction. The powder residue added to clay in the percentage of 0%, 5%, 10% and 15% (measured in weight) was compressed by a uniaxial pressing of 30MPa and the sintering temperatures were 900°, 1000° and 1100°. The samples were analysed in relation to flexural strength, bulk density, water absorption and linear shrinkage. The microstructural variation was also analysed by x-ray diffraction and electron microscopy. The results showed that there is a viability for the production of porcelain ceramic coating (A3 and A4 formulations) and stoneware (A2 formulation) according to the specification of technical standards. author)

  15. Magnetic resonance imaging textural evaluation of posterior cranial fossa tumors in childhood; Avaliacao textural por ressonancia magnetica dos tumores da fossa posterior em criancas

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Joelson Alves dos; Costa, Maria Olivia Rodrigues da; Otaduy, Maria Concepcion Garcia; Lacerda, Maria Teresa Carvalho de; Leite, Claudia da Costa [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Radiologia]. E-mail: joelson_alves@ig.com.br; Matsushita, Hamilton [Sao Paulo Univ., SP (Brazil). Faculdade de Medicina. Dept. de Neurologia

    2004-08-01

    Objective: To distinguish healthy from pathological tissues in pediatric patients with posterior cranial fossa tumors using calculated textural parameters from magnetic resonance images. Materials And Methods: We evaluated 14 pediatric patients with posterior cranial fossa tumors using the software MaZda to define the texture parameters in selected regions of interest representing healthy and pathological tissues based on T2-weighted magnetic resonance images. Results: There was a statistically significant difference between normal and tumoral tissues as well as between supposedly normal tissues adjacent and distant from the tumoral lesion. Conclusion: Magnetic resonance textural evaluation is an useful tool for determining differences among various tissues, including tissues that appear apparently normal on visual analysis. (author)

  16. Evaluation of poly(vinylpyrrolidone) and collagen by Low Field Nuclear Magnetic Resonance Spectroscopy; Avaliacao da polivinilpirrolidona e do colageno por ressonancia magnetica nuclear de baixo campo

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Paula de M.; Tavares, Maria I.B. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Inst. de Macromoleculas Professora Eloisa Mano]. E-mail: pmcosta@ima.ufrj.br

    2005-07-01

    Blends of natural and synthetic polymers represent a new class of materials with better mechanical properties and biocompatibility than those of the single components. Collagen and poly(vinylpyrrolidone) are well known for their important biological properties. The blending of collagen with poly(vinylpyrrolidone) makes it possible to obtain new materials in which strong interactions between the synthetic and biological components occur. Do to the excellent biocompatibility of these polymers, this blend has been much studied intending biomedical applications. And a one technique that can provide important information on molecular mobility, compatibility and even evaluate the interactions that can occur with these polymers is the Low Field Nuclear Magnetic Resonance Spectroscopy. Thus, the purpose of this work is to evaluate collagen and poly(vinylpyrrolidone) by Low Field Nuclear Magnetic Resonance Spectroscopy. From the values of relaxation times obtained, we can conclude that these materials have different interactions, and different mobility domains, confirming the heterogeneity and complexity of these materials. (author)

  17. Chemical and microbiological assessments of the multi mixture treated by gamma radiation; Avaliacao quimica e microbiologica da multimistura tratada por irradiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Goncalves, Cinthia Graciele

    2008-07-01

    In Brazil, the multi mixture have being used since the eighties as an alternative against severe infantile malnutrition of the poorest population. However, its use is still reason of controversies mainly due to: the presence of anti nutritional factors, the microbiological quality and the nutritional value. Considering the routine use of multi mixture in the region, this work aimed to evaluate samples of multi mixture were collected in the metropolitan area of the City of Belo Horizonte/MG for determining the anti nutritional factors (phytic and oxalic acids), the microbiological quality, the centesimal and mineral composition, and still the induced effect in these factors of the gamma radiation. For the analyses, the samples passed by the process of gamma irradiation at doses of: 2, 4, 6, 8 and 10 kGy and then were evaluated anti nutritional factors (phytic acid and oxalic), the microbiological quality (yeasts and molds, Salmonella, Coagulase positive Staphylococcus, Bacillus cereus, coliform to 45 deg C) to full percentage (moisture, ash, proteins, carbohydrates and lipids) and the efficiency of the method of Paramagnetic Electronic Resonance (EPR) in the detection of irradiated samples. The mineral composition of the samples was carried out by the irradiation by Neutronic Activation. The methods used were searched in literature. The obtained results suggest that the concentration of the phytic and oxalic acids can not be appropriated for the children with severe nutritional deficit and that it would be necessary additional control in their daily ingestion due to the absorption of essential minerals. In general the samples had presented acceptable microbiological quality for consumption, except by one of it. The data of the centesimal and mineral composition, in the usually recommended portions, showed lower concentration than recommended for children. Any significant alteration in phytic and oxalic acids as well as in the centesimal composition were detected after gamma irradiation. The electronic paramagnetic resonance can be detected irradiated samples. (author)

  18. Evaluation of absorbed radiation dose in mammography using Monte Carlo simulation; Avaliacao da dose absorvida em mamografia usando simulacao Monte Carlo

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Bruno L.; Tomal, Alessandra [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Instituto de Fisica Gleb Wataghin

    2016-07-01

    Mammography is the main tool for breast cancer diagnosis, and it is based on the use of X-rays to obtain images. However, the glandular tissue present within the breast is highly sensitive to ionizing radiation, and therefore requires strict quality control in order to minimize the absorbed dose. The quantification of the absorbed dose in the breast tissue can be done by using Monte Carlo simulation, which allows a detailed study of the deposition of energy in different regions of the breast. Besides, the results obtained from the simulation can be associated with experimental data and provide values of dose interest, such as the dose deposited in glandular tissue. (author)

  19. Exergy and thermoeconomic evaluation of hydrogen production from natural gas; Avaliacao exergetica e termo-economica da producao de hidrogenio a partir do gas natural

    Energy Technology Data Exchange (ETDEWEB)

    Cruz, Flavio Eduardo da [PROMON Engenharia Ltda., Sao Paulo, SP (Brazil); Oliveira Junior, Silvio de [Universidade de Sao Paulo (USP), SP (Brazil). Escola Politecnica

    2008-07-01

    Some specific processes are required to obtain pure hydrogen and the most usual one is the natural gas reforming, where natural gas reacts with superheated steam producing H{sub 2}, CO, CO{sub 2} and H{sub 2}O. This paper presents exergy and thermoeconomic analysis of a complete hydrogen production unit of a petroleum refinery. The hydrogen production unit analysed in this paper has to supply 550,000 Nm{sup 3} of hydrogen per day to purify diesel oil. Based on a synthesis plant of the hydrogen production unit, the exergy efficiency of each component and of the overall plant are calculated. The hydrogen production cost is determined by means of a thermoeconomic analysis in which the equality cost partition method is employed, including capital and operational costs, in order to determine the production cost of hydrogen and other products of the plant.(author)

  20. Evaluation of homogeneity and dose conformity in IMRT planning in prostate radiotherapy; Avaliacao da homogeneidade e conformidade de dose em planejamentos de IMRT de prostata em radioterapia

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Juliane S.; Leidens, Matheus; Estacio, Daniela R., E-mail: juliane.lopes@pucrs.br [Hospital Sao Lucas (PUC-RS), Porto Alegre, RS (Brazil). Servico de Radioterapia; Razera, Ricardo A.Z.; Streck, Elaine E.; Silva, Ana M.M. da [Pontificia Universidade Catolica do Rio Grande do Sul (PUC-RS), Porto Alegre, RS (Brazil). Faculdade de Fisica

    2015-12-15

    The goal of this study was to evaluate the dose distribution homogeneity and conformity of radiation therapy plans of prostate cancer using IMRT. Data from 34 treatment plans of Hospital Sao Lucas of PUCRS, where those plans were executed, were retrospectively analyzed. All of them were done with 6MV X-rays from a linear accelerator CLINAC IX, and the prescription doses varied between 60 and 74 Gy. Analyses showing the homogeneity and conformity indices for the dose distribution of those plans were made. During these analyses, some comparisons with the traditional radiation therapy planning technic, the 3D-CRT, were discussed. The results showed that there is no correlation between the prescribed dose and the homogeneity and conformity indices, indicating that IMRT works very well even for higher doses. Furthermore, a comparison between the results obtained and the recommendations of ICRU 83 was carried out. It has also been observed that the indices were really close to the ideal values. 82.4% of the cases showed a difference below 5% of the ideal value for the index of conformity, and 88.2% showed a difference below 10% for the homogeneity index. Concluding, it is possible to confirm the quality of the analyzed radiation therapy plans of prostate cancer using IMRT. (author)

  1. Importance of evaluation of uncertainties on the measurement of natural gas and petroleum volumes; Importancia da avaliacao das incertezas na medicao dos volumes de petroleo e gas natural

    Energy Technology Data Exchange (ETDEWEB)

    Silva Filho, Jose Alberto Pinheiro da; Oliveira, Thiago Barra Vidal de; Mata, Josaphat Dias da [PETROBRAS, Rio de Janeiro, RJ (Brazil)], Emails: jose.pinheiro@petrobras.com.br, thiagovidal@petrobras.com.br, josaphat@petrobras.com.br; Val, Luiz Gustavo do [Instituto de Qualidade e Metrologia (IQM), Rio de Janeiro, RJ (Brazil)], E-mail: gdoval.iqm@petrobras.com.br

    2009-07-01

    The measurement is considered as the 'cash register' of the enterprises, increasing the accuracy and the exigence at each step when come close to the delivery points, where the 0.1 % of differences are discussed. The work presents the approach used in the evaluation of measurement uncertainties in the volumes obtained of petroleum and natural gas at the processes of production in Brazil, and in the international level as well.

  2. Residual stress evaluation and curvature behavior of aluminium 7050 peen forming processed; Avaliacao da tensao residual em aluminio 7050 conformado pelo processo peen forming

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, R.R. de; Lima, N.B., E-mail: rolivier@ipen.b, E-mail: nblima@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Braga, A.P.V.; Goncalves, M., E-mail: anapaola@ipt.b, E-mail: mgoncalves@ipt.b [Instituto de Pesquisas Tecnologicas (IPT), Sao Paulo, SP (Brazil)

    2010-07-01

    Shot peening is a superficial cold work process used to increase the fatigue life evaluated by residual stress measurements. The peen forming process is a variant of the shot peening process, where a curvature in the plate is obtained by the compression of the grains near to the surface. In this paper, the influence of the parameters such as: pressure of shot, ball shot size and thickness of aluminum 7050 samples with respect to residual stress profile and resulting arc height was studied. The evaluation of the residual stress profile was obtained by sin{sup 2} {Psi} method. (author)

  3. Quantitative computed tomography as a test of endurance for evaluation of bony plates; Utilizacao da tomografia computadorizada quantitativa como teste de resistencia para avaliacao de placas osseas

    Energy Technology Data Exchange (ETDEWEB)

    Melo Filho, E.V.; Costa, L.A.V.S.; Oliveira, D.C. [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil); Freitas, P.M.C. [Escola de Veterinaria - Universidade Federal de Minas Gerais - Belo Horizonte, MG (Brazil); Teixeira, M.W.; Costa, F.S. [Universidade Federal Rural de Pernambuco - Recife, PE (Brazil)

    2012-06-15

    Quantitative computed tomography was used to determine the radiodensity of bony plates. The CT scans provided information regarding radiodensity of bony plates and allowed to verify the uniformity of bone mineral density in their scope. The proposed methodology should be considered as another tool for determining the resistance of these biomaterials. (author)

  4. Evaluation of the optical quality of compound parabolic concentrator solar collectors; Avaliacao da qualidade otica de coletores solares concentradores parabolicos compostos

    Energy Technology Data Exchange (ETDEWEB)

    Beyer, P.O.; Krenzinger, A. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Programa de Pos-graduacao em Engenharia Mecanica

    1990-12-31

    This work presents a simulation of solar compound parabolic concentrators using the ray tracing technique. The program can be used as a computer aided design and quality control applications for parabolic mirrors. (author). 4 refs., 8 figs.

  5. Evaluation of operational management in the oil terminals using human factor indicator; Avaliacao da gestao operacional em terminais com o uso do indicador de fator humano

    Energy Technology Data Exchange (ETDEWEB)

    Mendes, George L.D. [PETROBRAS, Rio de Janeiro, RJ (Brazil); Lima, Gilson Brito Alves [Universidade Federal Fluminense, Niteroi, RJ (Brazil). LATEC. Mestrado Profissional em Sistemas de Gestao

    2005-07-01

    This research has as objective to analyze the continuous improvement in a management system at TRANSPETRO in Madre de Deus Terminal (BA), environment and occupational health, particularly in the reduction of the accident levels. We consider relevant in this research the effective implementation of the human factor that includes the boarding of the work system relating them it three points: individual activities, organizational processes of work and activities, emphasizing the management of these resources and the communication between them. The case study it was make in a company of logistic of fuels. The methodology was lead through bibliographical research and applied closed questionnaire (adaptation's baseline API 770 - Manager's Guide to Reducing Human Errors Improving - Improving in the Process Industries) in 2003 and 2005 with the manager, coordinators, supervisors, operators and others technician. Were analyzed results of the company in management security and occupational health, such as: pointers of the tax of frequency of accidents with and without removal, volume of leaked product and results of internal and external audits. We conclude that the importance of the human factor in the Safety Management that propitiated significant progress in the organization during the development of the research. (author)

  6. Dose evaluation in medical staff during diagnostics procedures in interventional radiology; Avaliacao da dose na equipe medica durante procedimentos diagnoticos de radiologia intervencionista

    Energy Technology Data Exchange (ETDEWEB)

    Bacchim Neto, Fernando A.; Alves, Allan F.F.; Rosa, Maria E.D.; Miranda, Jose R.A. [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Instituto de Biociencias. Departamento de Fisica e Biofisica; Moura, Regina [Faculdade de Medicina de Botucatu, SP (Brazil). Departamento de Cirurgia e Ortopedia; Pina, Diana R., E-mail: bacchim@ibb.unesp.br [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Faculdade de Medicina. Departamento de Doencas Tropicais e Diagnostico por Imagem

    2014-08-15

    Studies show that personal dosimeters may underestimate the dose values in interventional physicians, especially in extremities and crystalline. The objective of this work was to study the radiation exposure levels of medical staff in diagnostic interventional radiology procedures. For this purpose LiF:Mg,Ti (TLD-100) dosimeters were placed in different regions of the physician body. When comparing with reference dose levels, the maximum numbers of annual procedures were found. This information is essential to ensure the radiological protection of those professionals. (author)

  7. Evaluation of quality control tools for patients submitted to IMRT; Avaliacao das ferramentas de controle da qualidade para pacientes submetidos ao IMRT

    Energy Technology Data Exchange (ETDEWEB)

    Lavor, Milton; Rodrigues, Laura N.; Silva, Marco A., E-mail: miltonlavor@gmail.com [Universidade de Sao Paulo (HCFMRP/USP), Sao Paulo, SP (Brazil). Hospital das Clinicas. Servico de Radioterapia

    2013-04-15

    Intensity modulated radiation therapy (IMRT) is currently being implemented in a rapidly growing number of centers in Brazil. As consequence many institutions are now facing the problem of performing a comprehensive quality control program before and during the implementation of IMRT in the clinical practice. This paper proposes a methodology for quality control and presents the results and evaluations of the data obtained from the proposed methodology. Ionization chamber and two-dimensional array detector were performed in IMRT treatment planning in order to assess the absolute value of the total dose of all fields. The relative total dose distribution of all fields was measured with a radiochromic film and a two-dimensional array in a phantom. A comparison between measured and calculated dose distributions was performed using the gamma-index method, assessing the percentage of points that meet the criteria of ±3% dose difference and ±3mm distance to agreement. As a result and review of 113 tested IMRT beams using ionization chamber and 81 using two-dimensional array, the proposal was to take an action level of about ±5% compared to the treatment planning systems and measurements, for the verification of the dose in a single point at the low gradient dose region. Analysis of the two-dimensional array measurements showed that the gamma value was <1 for 97.7% of the data and for the film the gamma value was <1 for 96.6% of the data. This work can establish action levels required for quality control program proposed and implemented in the Department of Radiotherapy - Hospital das Clinicas in Sao Paulo that allows an accurate delivery of dose in 'sliding-window' IMRT with micro multi leaf collimator. (author)

  8. Methodology for probability of failure assessment of offshore pipelines; Metodologia qualitativa de avaliacao da probabilidade de falha de dutos rigidos submarinos estaticos

    Energy Technology Data Exchange (ETDEWEB)

    Pezzi Filho, Mario [PETROBRAS, Rio de Janeiro, RJ (Brazil)

    2005-07-01

    In this study it is presented a methodology for assessing the likelihood of failure for every failure mechanism defined for carbon steel static offshore pipelines. This methodology is aimed to comply with the Integrity Management policy established by the Company. Decision trees are used for the development of the methodology and the evaluation of the extent and the significance of these failure mechanisms. Decision trees enable also the visualization of the logical structure of algorithms which eventually will be used in risk assessment software. The benefits of the proposed methodology are presented and it is recommended that it be tested on static offshore pipelines installed in different assets for validation. (author)

  9. Evaluation of absorbed radiation dose rate in a didactic X-ray equipment; Avaliacao da taxa de dose absorvida em um equipamento de raios-X didatico

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Phelipe Amaral Ferreira; Perini, Ana Paula; Neves, Lucio Pereira, E-mail: lucio.neves@ufu.br [Universidade Federal de Uberlandia (UFU), MG (Brazil). Instituto de Fisica

    2016-07-01

    This work was performed in order to create a new didactic experiment in the X-ray apparatus of PHYWE, where the saturation current was obtained through a free air ionization chamber. The values of saturation currents were obtained in two ways. Initially, the anodic DDP was kept constant and the anodic current was varied. In the second way, the anodic current was kept constant while the anodic DDP was varied. Therefore, we were able to evaluate the dependence of the absolved dose rate in relation to the DDP and the tube current. (author)

  10. Kerma rate evaluation in the air in a room interventional cardiology; Avaliacao da taxa de Kerma no ar em uma sala de cardiologia intervencionista

    Energy Technology Data Exchange (ETDEWEB)

    Real, Jessica V.; Luz, Renata M. da, E-mail: jessica.real@pucrs.br, E-mail: renata.luz@pucrs.br [Hospital Sao Lucas (HSL/PUCRS), Porto Alegre, RS (Brazil); Fröhlich, Bruna D.; Silva, Ana Maria Marques da, E-mail: bruna.frohlich@acad.pucrs.br, E-mail: ana.marques@pucrs.br [Pontificia Universidade Catolica do Rio Grande do Sul (PUCRS), Porto Alegre, RS (Brazil)

    2014-07-01

    In recent years, the number of interventional cardiology procedures is increasing. However, due to the long time of fluoroscopy in these procedures, care teams can receive high doses of radiation. The radiation scattered by the patient is not uniform, and their assessment is of utmost importance. This study aimed to estimate and map the kerma rate in the air at the time of the gonads, in an interventional cardiology room, seeking to optimize the dose absorbed by individuals occupationally exposed to ionizing radiation. For data collection, the room was divided into quadrants of 1m{sup 2}, totaling 40 collection points. The simulator was positioned so that its entry surface was located in the interventional reference point. Were chosen the conditions that simulate angiography and angioplasty procedures performed in the service. The data were obtained for height of 1 meter, gonad region. The results obtained for kerma rates in air, in quadrants, show that higher measured values was in the vicinity of the X-ray tube. Has been found that the medical staff are more exposed, because of its location during the procedure, around the table. The law of the inverse square distance of the farthest points of the X-ray tube were verified.

  11. Evaluation of the capacity of heavy metal adsorption in exfoliated vermiculite; Avaliacao da capacidade de adsorcao de metal pesado em vermiculitas esfoliadas

    Energy Technology Data Exchange (ETDEWEB)

    Lima, J.F.; Silva, P.S.; Hanken, R.B.L. [Universidade Federal de Campina Grande (UAEMa/UFCG), PB (Brazil). Unidade Academica de Engenharia de Materiais; Raposo, C.M.O., E-mail: raposo@dmg.ufcg.edu.b [Universidade Federal de Campina Grande (UAMG/UFCG), Bodocongo, PB (Brazil). Unidade Academica de Mineracao e Geologia

    2009-07-01

    Many groups from modern society have seen with attention the issues of pollutants, generally present in nature, those same that have caused irreversible damages to the environment. The Vermiculite, a phyllosilicate, with t-o-t structure, have high interlamelar charge, has been studied as cationic exchanger, whose application when exfoliated, are increased. This work has the objective of evaluate the absorption capacity of chromium (III), in different concentrations, in high, slim and medium concentrations of exfoliated vermiculites. The results obtained from the characterization by spectroscopy in infrared and by diffraction of x-ray from prepared solids showed important variations in the quantity of adsorbed metal in order the size of the concentrated particles. (author)

  12. Method for evaluating the applicability and application rate wastes in soil; Metodo para avaliacao da aplicabilidade e taxa de aplicacao de residuos em solo

    Energy Technology Data Exchange (ETDEWEB)

    Linhares, Monica Moreira [Partime (Brazil); Seabra, Paulo Negrais [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas

    1991-01-01

    Land treatment of refinery solid wastes has been practiced in the last few years by some PETROBRAS refineries. The biodegradation process is dynamic and complex, and incorrect monitoring may destroy the potential of the soil. Due to great differences both in terms of soils and of residues, each case must be monitored individually. We therefore developed a monitoring method for land farming systems based on the impact of the waste on indigenous soil microbial populations. The method is extremely simple, requiring only a gas chromatograph to evaluate the CO{sub 2} evolution, and can detect possible causes for low efficiency biodegradation processes. Conditions should then be altered, to ensure adequate functioning of the system.The method also allows for determination of the applicability of candidate wastes and of acceptable waste application rates in soil. (author) 2 refs., 7 figs.

  13. Evaluation of the life cycle of integrated production of ethanol and biodiesel; Avaliacao do ciclo de vida da producao integrada de etanol e biodiesel

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Simone Pereira de [Universidade Estadual de Campinas (UNICAMP), SP (Brazil)], email: sp.souza@yahoo.com.br; Pacca; Sergio Almeida [Universidade de Sao Paulo (USP), SP (Brazil). Escola de Artes, Ciencias e Humanidades], email: spacca@usp.br

    2010-07-01

    Sugarcane and palm are amongst the crops with the highest yields, which can reach 6 to 7.5 thousand liters of ethanol per hectare year and 4 to 6 metric tons of oil per hectare year, respectively. A joint production of biofuels from these crops is an interesting alternative for life cycle assessment improvement through fossil fuel and greenhouse gas emissions reduction, besides energy efficiency gains. The objective of this work is comparing the Brazilian production system sugarcane ethanol with an integrated system proposal where the ethanol and palm biodiesel are produced together. This comparative study is based on a life cycle approach using the ISO 14.044/2006 and appropriate indicators. Production systems in Cerrado, Cerradao and grassland ecosystem were considered. The energy balance, carbon balance and land use change were evaluated. The integrated system includes a 100% substitution of biodiesel by diesel, which is used in life cycle agriculture stages. Distinct fractions of sugarcane used for sugar production were assessed. When all sugarcane is sent to sugar production, ethanol is produced by molasses. The data were collected by direct observation method by questionnaire and secondary sources. Three sugarcane mills situated in Sao Paulo state and a palm mill located in Para state were surveyed. Results showed a 164% increase in energy balance for the joint production system in comparison to the traditional system. Besides this, the joint production system demonstrated a 24% GHG emission reduction. For ethanol production by molasses, the energy balance was up to 59% and 162% higher in scenarios 1 and 2, respectively, which differ each other through of the boiler that was utilized. For GHG emission, the reduction can reach 91%. The land use change identify an average ecosystem carbon payback time for Cerrado, Cerradao and Degraded Grassland of 5, 10, and -5 years, respectively. In conclusion, the joint production system of sugarcane ethanol and palm biodiesel presents an improvement in energy balance, GHG emissions and land use change impacts in comparison to the traditional ethanol system. (author)

  14. Chemical constituents and evaluation of antibacterial activity of Macroptilium lathyroides (L.) Urb. (Fabaceae); Constituintes quimicos e avaliacao da atividade antibacteriana de Macroptilium lathyroides (L.) Urb. (Fabaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Sousa, Leoncio M. de; Gois, Roberto W. da S.; Lemos, Telma L. G.; Arriaga, Angela M. C.; Andrade-Neto, Manoel [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Departamento de Quimica Organica; Santiago, Gilvandete M.P., E-mail: gil@ufc.br [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Departamento de Farmacia; Braz-Filho, Raimundo [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, RJ (Brazil). Centro de Ciencias e Tecnologias; Costa, Jose G.M. da; Rodrigues, Fabiola F.G. [Universidade Regional do Cariri, Crato, CE (Brazil). Departamento de Quimica Biologica

    2013-11-01

    The chemical investigation of the stems and branches of Macroptilium lathyroides led to the isolation of a mixture of {beta}-sitosterol and stigmasterol. The extracts from the roots allowed the isolation of lasiodiplodin, a mixture of stigmast-4-en-6{beta}-ol-3-one and stigmast-4,22-dien-6{beta}-ol-3-one, de-O-methyllasiodiplodin, genistein and lupinalbin A. The structures of the isolated compounds were assigned on the basis of their NMR data, including comparison of their spectral data with values described in the literature. The antibacterial activity of crude extracts from stems, branches and roots was evaluated. This is the first report involving the chemical investigation of this species. (author)

  15. Biodegradation evaluation of recycled polyethylene doped with Moringa oleifera oil; Avaliacao da biodegradacao de filmes de polietileno reciclado dopados com oleo de Moringa oleifera

    Energy Technology Data Exchange (ETDEWEB)

    Bicalho, Luciana A.; Novack, Katia M.; Melo, Tania M.S., E-mail: knovack@iceb.ufop.br [Dept. de Quimica (DEQUI) - Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG (Brazil)

    2011-07-01

    Chemical modifications and use of additives are some of the mainly ways to obtain polymer materials with especial properties. Vegetable oils incorporated to polymers preserve their structure while make possible a reduction of degradation rate. This work proposed the use of polyethylene, one of the most common polymer commodities, because of its low cost and wide application. In this project it was verified the possibility of making polymer materials with properties of a natural product through the incorporation of Moringa oleifera oil to recycled low density polyethylene (LDPEr) in different proportions. The films were buried for different times and their degradation was evaluated.) Samples were characterized by DSC, TGA and XRD. It was observed that samples doped with Moringa oil showed lower degradation time. (author)

  16. Evaluation of radiation effects on dental enamel hardness and dental restorative materials; Avaliacao do efeito da irradiacao na dureza do esmalte dental e de materiais odontologicos

    Energy Technology Data Exchange (ETDEWEB)

    Adachi, Lena Katekawa; Saiki, Mitiko [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Supervisao de Radioquimica; Campos, Tomie Nakakuki [Sao Paulo Univ., SP (Brazil). Faculdade de Odontologia. Dept. de Protese

    2000-07-01

    This research presents the results of the microhardness of human dental enamel and of the following dental restorative materials: three dental porcelains - Ceramco II, Finesse and Noritake, and two resin restorative materials - Artglass and Targis, for materials submitted to different times of irradiation at the IEA-R1m nuclear reactor under a thermal neutron flux of 10{sup 12}n cm{sup -2}.s{sup -1} . The results obtained indicated that there is a decrease of the surface microhardness when the enamel is irradiated for 1 h and when dental materials are irradiated for 3 h. However, enamels irradiated for 30 min. did not show significant change of their surface hardness. Therefore, the selection of irradiation time is an important factor to be considered when irradiated teeth or dental materials are used in the investigations of their properties. (author)

  17. Technological evaluation of fuel cells using natural gas for distributed power generation; Avaliacao tecnologica da utilizacao de gas natural em celulas a combustivel para geracao distribuida de energia

    Energy Technology Data Exchange (ETDEWEB)

    Brandao, Mauricio O. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao de Engenharia (COPPE). Programa de Engenharia Mecanica; Giannini, Marcio P.; Arouca, Mauricio C. [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao de Engenharia (COPPE). Programa de Planejamento Energetico

    2004-07-01

    The search for sustainable and more rational means of power generation motivates the scientific crew to search for more efficient and cleaner systems. Oil dependence becomes from the kind of development that the humanity had and cannot be dismissed. The question is how to use this source in a more intelligent way. Fuel Cells are electrochemical devices that convert into electric energy the chemical energy from oxi-reduction reactions between a fuel and an oxidant. The current fuel used in a Fuel Cell is hydrogen and oxygen is the oxidant. The great advantage of this device is its efficiency, higher than the one achieved with internal combustion engines. Also Fuel Cells are not limited by Carnot's efficiency. This paper is about the implementation of a distributed generation system using Fuel Cells. Technical aspects are approached together with economical and environmental needs. The already existence of Gas pipelines and the grown production of Natural Gas presented by Brazil turns it into a good market for the implementation of this energy source. The evaluation of this paper shows that is technically possible to use NG in Fuel Cells, mostly in South and Southeast regions, applying the distributed generation of energy concept. The most interesting in a strategic manner is that Brazil already have an indication that it's capable of developing this technology, opening a new market tuning with world's new technological developments. Many research centers develop this technology, not only from the cell composition itself, but also manufacturing techniques. (author)

  18. Evaluation of thermal stability of paraffin wax by differential scanning calorimetry; Avaliacao da estabilidade termica de parafina por calorimetria diferencial de varredura

    Energy Technology Data Exchange (ETDEWEB)

    Godinho, K.O.; Silva, A.G.P.; Holanda, J.N.F. [Universidade Estadual do Norte Fluminense (LAMAV/UENF), Campos dos Goytacazes, RJ (Brazil). Grupo de Materiais Ceramicos], Email: holanda@uenf.br

    2010-07-01

    Phase change materials for heat storage are used as passive solar energy storage materials, which can be impregnated into construction materials. In this work the thermal stability (heating/cooling cycle) of the paraffin wax was investigated using differential scanning calorimetry. The latent heat and fusion temperature were determined for the following thermal cycles: 0, 30, 180 and 360. The thermal stability for paraffin wax infiltrated in support of gypsum was also determined. The experimental results showed that the paraffin wax showed good thermal stability in the states pure and infiltrated for up to 360 thermal cycles. (author)

  19. Evaluation of influence of fertilizers in radium concentration from tomato culture; Avaliacao da influencia dos fertilizantes nos teores de radio encontrados na cultura do tomate

    Energy Technology Data Exchange (ETDEWEB)

    Lauria, Dejanira C. [Instituto de Radioprotecao e Dosimetria (IRD), Rio de Janeiro, RJ (Brazil). Dept. de Protecao Radiologica Ambiental; Ribeiro, Fernando C.A.; Alleluia, Irene B. [Instituto Nacional de Tecnologia (INT), Rio de Janeiro, RJ (Brazil). Div. de Meio Ambiente; Perez, Daniel V. [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Rio de Janeiro, RJ (Brazil). Lab. de Analises de Solos e Plantas

    2001-07-01

    The fertilizer contribution to radio uptake by tomato plants was assessed. Tomato and soil samples from four different plantations were analyzed: two using organic fertilizer and two using phosphate fertilizer. The Ra concentrations in eight of the most used tomato fertilizers were determined. The concentration values ranged from 14 to 221 Bq/kg of Ra-226 and from 25 to 176 Bq/kg of Ra-228. Owing to the low Ra concentration and to the used fertilizer quantities, the fertilizers would not increase significantly the Ra soil concentration. However, the highest soil to plant concentration ratios were found in the plantations using phosphate fertilizers. This outcome pointed out the higher Ra availability in the phosphate fertilizer plantations than in the organic fertilizer plantations, showing a possible positive influence of phosphate fertilizers for the tomato Ra uptake. Due to the phosphate fertilizer used, the dose could increase from 0,05 Sv/year to 0,24 Sv/year. However this increase is not significant face the ingestion average worldwide effective dose. (author)

  20. A regulatory model for conformity evaluation in natural gas building installations; Um modelo regulatorio para avaliacao da conformidade das instalacoes prediais de gas natural

    Energy Technology Data Exchange (ETDEWEB)

    Fossa, Alberto Jose; Santos, Edmilson Moutinho dos [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Eletrotecnica e Energia

    2008-07-01

    The challenge of the Brazilian growth needs to consider necessarily the energy arrangements, and natural gas has relevant participation in this matter. The possibility of its end use that make possible an effective adequacy of our energy matrix must be supported by modern concepts of quality and conformity. In this particular, the program of 'Tecnologia Industrial Basica' (TIB), including concepts and application of conformity evaluation processes, is considered basic for the construction of a consistent gas market. This paper present the Brazilian reality related to TIB aspects and elements, from which it constructs a technician and regulatory building gas installations model proposal for a conformity evaluation program in the country. (author)

  1. Analysis of an in-line diesel production system through event driven simulation; Avaliacao do esquema de producao em linha de diesel atraves da simulacao por eventos discretos

    Energy Technology Data Exchange (ETDEWEB)

    Monteiro, Gilsa P.; Naegeli, Guilherme S.T. [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES); Santos, Nilza M.Q. [PETROBRAS S.A., Mataripe, Salvador, BA (Brazil). Refinaria Landulfo Alves (RLAM); Netto, Joaquim D.A. [DNV Energy Solutions, Porto Alegre, RS (Brazil)

    2008-07-01

    The interactions between refining processes (such as distillation, hydrotreatment, etc.) and typical transfer and storage operations (mixtures, decantation, storage, etc.) provide a high complexity to the refineries production systems of petroleum derivatives. These production systems are characterized by many aspects, such as: blending rules, feed composition, petroleum campaigns, storage tanks limitations, continuous and batch processes interactions, etc. Besides these operational aspects, the equipment and systems' reliability has strong influence on the level of production goals achievement and petroleum derivatives quality specification. Looking for a higher economic efficiency and in order to provide refineries with orientation about resources optimization for their petroleum derivatives' production systems, the development of a methodology capable of being applied since the design phase to identify systems limitations and improvement opportunities, considering all the raised aspects, is a very important task. With this objective, this article presents the main points of an evaluation that was conducted during the conceptual design for a diesel in-line blending production system proposed by a Brazilian refinery, detailing the main steps of the methodology that was developed through this analysis, based on discrete event simulation. (author)

  2. Evaluation of biodegradation of bionanocomposites of PHB/PP-g-MA/vermiculite; Avaliacao da biodegradacao de bionanocompositos de PHB/PP-g-MA/vermiculita

    Energy Technology Data Exchange (ETDEWEB)

    Mesquita, P.J.P. de; Alves, T.S.; Barbosa, R., E-mail: tsaeng3@yahoo.com.br [Universidade Federal do Piaui (UFPI), Teresina, PI (Brazil). Programa de Pos-Graduacao em Ciencia dos Materiais; Andrade, D. de L.A.C.S. [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Programa de Pos- Graduacao em Ciencia e Engenharia de Materiais

    2014-07-01

    In this work, we evaluated the process of biodegradation of PHB bionanocomposites/PP-g-MA/clay. Films were prepared with compositions of 5% PP-g-MA and 3% clay (BIO5); and 2.5% PP-g-MA and 3% clay (BIO2.5). The biodegradation test was conducted according to ASTM G 160-03 for a period of 86 days. The evaluation of biodegradation was performed by optical microscopy (MO), loss of mass, FTIR and XRD. The bionanocomposites presented change of coloration and appearance of fissures. As for loss in mass in the system presented loss of BIO2,5 22.5% and 25.9% BIO5, for 86 days of simulated soil exposure. We also observed by FTIR sharp reduction in intensity of the band corresponding to the C = O groups, indicating that the microbial attack promoted cleavage of the ester group links. By XRD, reduction in the intensity of the peaks and enlargements of the same with increasing exposure time, proving the biodegradation behavior of bionanocomposites. (author)

  3. Evaluation of the normal thoracic and abdominal aorta diameters by computerized tomography; Avaliacao dos diametros normais da aorta toracica e abdominal pela tomografia computadorizada

    Energy Technology Data Exchange (ETDEWEB)

    Lucarelli, Claudio Luiz

    1995-07-01

    The study was undertaken to evaluate, through computerized tomography, the diameters of the normal thoracic and abdominal aorta, as well as they are connected to gender, age and body surface area; and the ratio between measurements obtained at the ascending and descending limbs of the thoracic aorta, and between the abdominal aortic diameters. For that reason, we measured the widest anteroposterior diameters of the thoracic aortas at the levels of the arch, the root, the pulmonary artery, and the thoracic-abdominal transition, as well as the level of the emergence of the superior mesenteric artery, of the renal hila and just cephalad to the bifurcation of the abdominal aortas of 350 patients without cardiovascular diseases who had undergone computerized tomography of the thorax and/or abdomen for any other reasons. Observation and statistic analyses led us to conclude that: 1) both the thoracic and abdominal aortic diameters are reduced from their proximal to their distal portions; 2) the body surface influences the size of the aorta, although only extreme variations alter the vessel's caliber; 3) vessel diameter was observed to gradually increase with age; 4) men were found to have larger diameters than age matched women; 5) the ratio between the ascending and descending aortic diameters varies according to gender and age; 6) the relations between abdominal aortic diameters measured at the level of renal hila and cephalad to the bifurcation are independent from gender and age. But the relation between those measured at the level of the superior mesenteric artery and cephalad to the bifurcation are linked to gender, but not to age. (author)

  4. Synthesis and antimicrobial activities of new 4-thiazolidones derived from formipyridine thiosemicarbazones; Sintese e avaliacao da atividade antimicrobiana de novas 4-tiazolidinonas obtidas a partir de formilpiridina tiossemicarbazonas

    Energy Technology Data Exchange (ETDEWEB)

    Vercoza, George Leonardo; Feitoza, Danniel Delmondes; Alves, Antonio Jose; Aquino, Thiago Mendonca de; Lima, Jose Gildo de [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Ciencias Farmaceuticas], e-mail: jgildolima@gmail.com; Araujo, Janete Magali; Cunha, Ivana Glaucia B.; Goes, Alexandre Jose da Silva [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Antibioticos

    2009-07-01

    Twelve novel 4-thiazolidinone derivatives (2a-l) have been synthesized by reacting formylpyridine thiosemicarbazones (1a-l) and anhydride maleic in toluene. Their chemical structures were confirmed by IR, {sup 1}H and {sup 13}C NMR. The new compounds were submitted to in vitro evaluation against pathogenic Gram-positive, Gram-negative bacteria and yeasts. The findings obtained showed that the compounds 2a, 2d, 2e and 2g were effective against some of the bacterial strains used, whereas the compounds 2d, 2e and 2i exhibited a moderate antifungal activity against the yeast strains evaluated. An initial structure activity relationship (SAR) was established. (author)

  5. Critical evaluation of the external occupational exposure in nuclear medicine services in Brazil; Avaliacao critica da exposicao ocupacional externa nos servicos de medicina nuclear no Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Kubo, Ana Luiza Silva Lima

    2016-07-01

    Currently in Brazil (2016), there are 421 Nuclear Medicine Services (NMS). In nuclear medicine, the possibility of occupational internal contamination and external exposure is unavoidable. The chest individual monitoring, to estimate the effective dose, is mandatory, but the extremity monitoring is not always made. The aim of this study was to conduct a survey of data for external exposure of NMS professionals in Brazil from 1987 to 2010, analysing them in terms of trends and comparing them with measurements carried out in this work and in other countries. Although most of the NMS is still located in large urban centres (54% in the Southeast region), there is no state without any NMS. The increasing number of NMS has generated the need for more professionals. In the year 1987, they were 755 workers and, in 2010, 4134, with the following distribution of specialties: 29% of Nuclear Medicine Technicians (NMT), 23% of Nursing professionals, 29% of Physicians and 3% of Physicists. The average annual effective dose reached more than 3.0 mSv in some regions of the country, from 1987 to 2010, but tends to 1.0 mSv in 2010. The highest doses, as expected, are received by NMT and Nursing. The professionals who handle radiopharmaceuticals have their hands much more exposed than the chest. During 2010, only 31% of NMT and 16% of Nursing used extremity dosimeters as compared to chest dosimeters. The data from the measurements indicate that not all individual dosimeters are used properly. Generally, both in the measurements as in national registries, the hand doses were higher for professionals who prepared the radiopharmaceutical (NMT) than those who injected (Nursing). The value measured by chest dosimeters can be used to estimate the equivalent dose to the eye lenses, except for NMT at preparation practices at conventional NMS, where the equivalent dose of the lens is about 2 times higher than the dose at the chest. The most exposed areas of the hands are the tips of the index and middle fingers of the non-dominant hand for professionals who carry out preparation of the radiopharmaceutical and the index finger of the dominant hand for those who perform injection in conventional NMS and making fractionation and injection in the case of Positron Emission Tomography (PET). Thus, measurements with dosimetric rings estimate better the extremity equivalent dose than bracelets, which can underestimate the hand equivalent dose by a factor between 10 and 20. Unlike the effective dose, the possibility that an occupational equivalent dose value exceeds the annual limit is real. The ability of the operator and the use of shielding seem to be more important to the magnitude of the dose than the used fractionation technique, which is highly relevant for the dose distribution in the hands. (author)

  6. Evaluation of the normal thoracic and abdominal aorta diameters by computerized tomography; Avaliacao dos diametros normais da aorta toracica e abdominal pela tomografia computadorizada

    Energy Technology Data Exchange (ETDEWEB)

    Lucarelli, Claudio Luiz

    1995-07-01

    The study was undertaken to evaluate, through computerized tomography, the diameters of the normal thoracic and abdominal aorta, as well as they are connected to gender, age and body surface area; and the ratio between measurements obtained at the ascending and descending limbs of the thoracic aorta, and between the abdominal aortic diameters. For that reason, we measured the widest anteroposterior diameters of the thoracic aortas at the levels of the arch, the root, the pulmonary artery, and the thoracic-abdominal transition, as well as the level of the emergence of the superior mesenteric artery, of the renal hila and just cephalad to the bifurcation of the abdominal aortas of 350 patients without cardiovascular diseases who had undergone computerized tomography of the thorax and/or abdomen for any other reasons. Observation and statistic analyses led us to conclude that: 1) both the thoracic and abdominal aortic diameters are reduced from their proximal to their distal portions; 2) the body surface influences the size of the aorta, although only extreme variations alter the vessel's caliber; 3) vessel diameter was observed to gradually increase with age; 4) men were found to have larger diameters than age matched women; 5) the ratio between the ascending and descending aortic diameters varies according to gender and age; 6) the relations between abdominal aortic diameters measured at the level of renal hila and cephalad to the bifurcation are independent from gender and age. But the relation between those measured at the level of the superior mesenteric artery and cephalad to the bifurcation are linked to gender, but not to age. (author)

  7. Bacteriological evaluation of fresh chicken sausage submitted to gamma radiation; Avaliacao bacteriologica da linguica de frango Frescal submetida a radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Borges, Alexandre

    2002-07-01

    Many developed countries have established a goal of self-sufficiency in food production, while many other obtain their principal foreign exchange through food exports. For these reasons, it is essential to eliminate losses in food products. It is no less important to prevent or combat food-transmitted diseases, principally across poultry and poultry derivatives, which play a major role in certain infections and is the focus of national public health goals in order to increase the international commerce. In tropical countries, perishable products represent the largest markets. The population lives in a constant battle against the deterioration of these foods. Such loss is enormous where the climate favors the proliferation of agents of decomposition and acceleration of putrification. Increasing the useful life of these perishable products will provide producers the option of commercializing them in low harvest season, thus achieving higher prices, and providing sensible growth to the agro-industrial market. Treatment of perishables with gamma radiation presently constitutes one of the most modern, safe, and efficient methods for increasing food preservation. The irradiation of foods can offer various special advantages such as: kill or sterilize pathogenic organisms in food, improving their quality and duration, reducing the level of toxics, slow or stop the aging of roots and tubers, disinfect products of animal and vegetable origin thus slowing their decomposition, increasing their shelf-life, and prolonging their conservation refrigeration. The practice of irradiating foods for the treatment of microorganisms has begun to gain momentum in Brazil, where roughly 25% of all food produced annually is lost to disease and insects activity, increased by lack of adequate storage treatments. Various countries have already adopted the method that, when practiced properly by qualified professionals, does not cause side effects other than the extension of useful life. In light of the lack of present data on the irradiation of fresh chicken sausage, the objective of this study is to evaluate determine the efficiency of this method as a process for reducing the microbial load via measurement of the bacteriology of the product before and after gamma radiation. 45 sample of chicken sausage(acquired directly from the poultry industry) were prepared divided in 3 treatments: the first is a control, the second and third using application of 1,5 and 3,0 kGy, respectively. The chicken sausages irradiated with a dose of 1,5 kGy did not have, statistically significant results apparent in the bacteriological measures. Samples irradiated with 3,0 kGy did experience statistically significant reductions. It is concluded that the irradiating the product with a dose of 3,0 kGy is more effective for the reduction of bacterial population, with the data in these trials indicating a 95,3% reduction in total coliforms, 100% removal of fecal coliforms, and 84,0% reduction of mesophiles, relative to the control samples. (author)

  8. Situational quality evaluation of mammography services at state of Minas Gerais, Brazil; Avaliacao situacional da qualidade dos servicos de mamografia do estado de Minas Gerais, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Joana, Georgia S.; Oliveira, Mauricio de; Andrade, Mauricio C. de; Cesar, Adriana C.Z., E-mail: georgia.santos@saude.mg.gov.b, E-mail: mauricio.cavalcanti@saude.mg.gov.b, E-mail: adrianac@saude.mg.gov.b, E-mail: mauricio.oliveira@saude.mg.gov.b [Secretaria de Estado da Saude de Minas Gerais (SVS/SES-MG), Belo Horizonte, MG (Brazil). Superintendencia de Vigilancia Sanitaria; Oliveira, Marcio A.; Nogueira, Maria do S., E-mail: marcio.alves@saude.mg.gov.b, E-mail: mnogue@cdtn.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Peixoto, Joao E. [Instituto Nacional de Cancer (INCA), Rio de Janeiro, RJ (Brazil)

    2011-07-01

    Breast cancer is the leading cause of cancer deaths in women. Currently, the most effective method for early detection of this cancer is the mammography, and to achieve the standard definition and contrast, the whole system of imaging must operate under optimal conditions. This paper presents the results of the assessment of mammography centers in the state of Minas Gerais, which was held with the aim of supporting the actions of the State Program of Quality Control in Mammography. These results indicated that less than half of mammography achieved the minimum standard of image quality, endorsing the need of a monitoring more efficient and effective, which led to the establishment, in Minas Gerais, of the monthly monitoring of image quality in mammography. (author)

  9. Comparison of the automated evaluation of phantom mama in digital and digitalized images; Comparacao da avaliacao automatizada do phantom mama em imagens digitais e digitalizadas

    Energy Technology Data Exchange (ETDEWEB)

    Santana, Priscila do Carmo, E-mail: pcs@cdtn.b [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear. Programa de Pos-Graduacao em Ciencias e Tecnicas Nucleares; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Fac. de Medicina. Dept. de Propedeutica Complementar; Gomes, Danielle Soares; Oliveira, Marcio Alves; Nogueira, Maria do Socorro, E-mail: mnogue@cdtn.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2011-07-01

    Mammography is an essential tool for diagnosis and early detection of breast cancer if it is provided as a very good quality service. The process of evaluating the quality of radiographic images in general, and mammography in particular, can be much more accurate, practical and fast with the help of computer analysis tools. This work compare the automated methodology for the evaluation of scanned digital images the phantom mama. By applied the DIP method techniques was possible determine geometrical and radiometric images evaluated. The evaluated parameters include circular details of low contrast, contrast ratio, spatial resolution, tumor masses, optical density and background in Phantom Mama scanned and digitized images. The both results of images were evaluated. Through this comparison was possible to demonstrate that this automated methodology is presented as a promising alternative for the reduction or elimination of subjectivity in both types of images, but the Phantom Mama present insufficient parameters for spatial resolution evaluation. (author)

  10. Impact of an evaluation quality program of mammography service of Distrito Federal - Brasil; Impacto de um programa de avaliacao da qualidade dos servicos de mamografia do Distrito Federal

    Energy Technology Data Exchange (ETDEWEB)

    Correa, Rosangela da Silveira

    2002-07-01

    Cancer is the second cause of death for Brazilian women and breast cancer is the most common neoplasm amongst women. Mammography is an essential tool for diagnosis and early detection of this disease. In order to be effective, the mammography must be of good quality. This study sought to evaluate the quality of these services in the Federal District and the impact of an intervention involving inspection and education. The universe of 41 mammography services in the Federal District was studied between June and August 2001. Two instruments were used 1) a proposed evaluation protocol of the National Agency for Health Surveillance (ANVISA) and 2) a protocol for evaluation of image quality and care. The intervention included the initial inspection, a training activity, and a formal notification by the health authorities of the Federal District. Image quality was compared before and after the intervention. The existing infrastructure in the DF is of good quality, but the services have low productivity, they do not treat patients sent by the public health system, they have an inadequate geographic distribution with most in the central neighborhood, and the low income female population has restricted access to early detection of cancer. Of the 36 services who completed the study, none was above 90% conformity in image quality before the interventions, whereas 10 were above 90% afterwards. The greatest improvements were in chassis maintenance (services without chassis with defects went from 10 to 25), breast compression (services in conformity went from 7 to 20) and visualization of micro calcifications (32 services in conformity before and 35 afterwards). The results of the ANVISA protocol bore no relation to final image quality, a central issue for early cancer detection. In spite of the existence of many services, most were not of quality and population access was restricted. The intervention under study was shown to be effective for improving quality, nevertheless ongoing action is needed to resolve the remaining problems and to increase the impact. The proposed method for public monitoring should be modified to evaluate primarily the quality of the final exam provided to the consumer. (author)

  11. Residual stress evaluation and curvature behavior of aluminum 7050 peen forming processed; Avaliacao da tensao residual em aluminio 7050 conformado pelo processo peen forming

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Rene Ramos de

    2011-07-01

    Shot peening is a superficial cold work process used to increase the fatigue life evaluated by residual stress measurements. The peen forming process is a variant of the shot peening process, where a curvature in the plate is obtained by the compression of the grains near to the surface. In this paper, the influence of the parameters such as: pressure of shot, ball shot size and thickness of aluminum 7050 samples with respect to residual stress profile and resulting arc height was studied. The evaluation of the residual stress profile was obtained by sin{sup 2} {psi} method. The results show that the formation of the curvature arc height is proportional to the shot peening pressure, of spheres size and inversely proportional to the thickness of the sample, and that stress concentration factor is larger for samples shot peened with small balls. On final of this paper presents an additional study on micro strain and average crystallite size, which can evaluate the profile of the samples after blasting. (author)

  12. Evaluation of the strengthening of the conditions of voltage security using sensitivity analysis; Avaliacao do reforco das condicoes da seguranca de tensao utilizando a analise de sensibilidade

    Energy Technology Data Exchange (ETDEWEB)

    Rosa, A.L.S.; Costa, V.M. da; Peres, W. [Universidade Federal de Juiz de Fora (UFJF), MG (Brazil). Faculdade de Engenharia], Emails: arleilucas@gmail.com, vander@lacee.ufjf.br, wesley.peres@yahoo.com.br; Prada, R.B. [Pontificia Universidade Catolica (PUC-Rio), Rio de Janeiro, RJ (Brazil). Dept. de Engenharia Eletrica], Email: prada@ele.puc-rio.br

    2009-07-01

    Voltage stability or voltage security analysis has motivated an expressive attention of power systems researchers. In this work, a simple and fast method evaluates voltage security and proposes conditions to improve the loading margin. System analysis is carried out in terms of active power transmission path. Sensitivity analysis provides the most adequate buses to active and reactive power redispatch. A sequential iterative methodology to reinforce system conditions is presented. (author)

  13. Monoamine levels in the nucleus accumbens correlate with male sexual behavior in middle-aged rats.

    Science.gov (United States)

    Tsai, Houng-Wei; Shui, Hao-Ai; Liu, Hang-Shen; Tai, Mei-Yun; Tsai, Yuan-Feen

    2006-02-01

    The correlation between monoamine levels in the nucleus accumbens (NAcc) and male sexual behavior was studied in middle-aged rats. Male rats (18-19months) were assigned to three groups: (1) Group MIE consisted of rats showing mounts, intromissions, and ejaculations; (2) Group MI was composed of rats showing mounts and intromissions, but no ejaculation; and (3) Group NC were non-copulators showing no sexual behavior. Young adult rats (4-5months), displaying complete copulatory behavior, were used as the control group. Levels of dopamine (DA), serotonin, and norepinephrine and their metabolites in the NAcc were measured by high-pressure liquid chromatography with electrochemical detection. No difference was seen in DA levels between MIE rats and young controls, whereas DA levels in NC rats were significantly lower than those in both MIE and MI rats. Serotonin levels in NC rats were significantly higher than those in MIE and MI rats. Conversely, norepinephrine levels in NC rats were lower than those in MIE rats. These results suggest that monoamine levels in the NAcc correlate with sexual performance in male rats and that changes in NAcc monoamine levels might affect male sexual behavior in middle-aged rats.

  14. Methymazole (MMI) effects on seric levels of thyroid hormones in rats and mices; Influencia do metimazol (MMI) sob os niveis sericos dos hormonios da tireoide em rato e camundongos

    Energy Technology Data Exchange (ETDEWEB)

    Lima Filho, G.L.; Carvalho, E.B.; Lima, G.M.S.; Neves, S.R.S.; Catanho, M.T.J.A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia; Lima, G.M.T. [Pernambuco Univ., Recife, PE (Brazil). DMC

    1997-12-01

    The thyroid gland secretes the metabolic hormones thyroxine (T4) and triiodothyronine (T3) which regulate the oxygen consumption of the majority of cells in the body. Their synthesis and release are controlled by the anterior pituitary hormone and also for drugs that mediated the serum concentration of T4 and T3 in the thyroid gland or in the peripheral tissues. The present study evaluates the sinergic effect on the basal secretion of the T4 and T3 after administration of throidal and nonthyroidal drugs in rats and mice. The study achievements with the oral administration of methymazole (MMI) in rats and mice. The study achievements with the oral administration of Methymazole (MMI) in rats and mice resulted in the reduction of the T4 and T3 serum levels, obtained through kinertic treatment. there was a significant reduction in T4 serum values among treated rats and mice for up to 14 days of MMI. Moreover, increased T3 serum concentration was found in rats treated with MMI, after 7 days of treatment, when compared to the serum level of treated mice. The serie levels of T3 and T4 were determined by radioimmunoassay. (author). 11 refs., 2 figs.

  15. Effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing: experimental study in rats Efeitos da membrana de hialuronato de sódio e carboximetilcelulose na formação de colágeno e fibroblastos no processo de cicatrização de colorrafias: estudo experimental em ratos

    Directory of Open Access Journals (Sweden)

    Antônio Carlos Perez

    2005-02-01

    Full Text Available PURPOSE: To analyze the effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing in rats. METHODS: 48 male Wistar rats, weighing 250 to 343g, were randomized into two groups: group I - bowel suture without applying a biologically absorbable membrane and group II - bowel suture with application of an absorbable membrane. The two groups were divided into subgroups of 3, 14 and 30 days of observation, with 8 rats in each subgroup. All were sacrificed after the end of the observation period. RESULTS: No morbidity or mortality was observed during the experiment. The amounts of collagen in group I were 23.4%, 72.1% and 67.6% and in group II were 22.5%, 52.5% and 51.6%, for the subgroups of 3, 14 and 30 days, respectively. Comparison between groups showed that the 14-day (p=0.0013 and 30-day (p=0.0587 subgroups had significant variance, with larger collagen zones in animals in which the membrane was not applied. However, with regard to fibroblasts, group I had 2%, 13% and 8% and group II had 2%, 10% and 8%, for the 3-day (p=1.0, 14-day (p=0.3184 and 30-day (p=0.5995 subgroups, respectively, showing no significant variance. CONCLUSION: The use of the biologically absorbable membrane cause a decrease in collagen formation, while not altering the number of fibroblasts, in bowel suture healing in rats, without increased morbidity and mortality.OBJETIVO: Analisar os efeitos da membrana de hialuronato de sódio e carboximetilcelulose, na formação de colágeno e fibroblastos na colorrafia de ratos. MÉTODOS: Foram utilizados 48 ratos machos da linhagem Wistar, com peso entre 250 e 343g, distribuídos em dois grupos: grupo I colorrafia sem aplicação de membrana bioabsorvível e grupo II colorrafia com aplicação de membrana bioabsorvível; tendo sido divididos em subgrupos de 3, 14 e 30 dias de observação, com 8 animais em cada um dos subgrupos, todos submetidos à eutanásia após o

  16. Efeitos da exposição à fumaça lateral do cigarro sobre o barorreflexo em ratos adultos Efectos de la exposición al humo lateral del cigarrillo sobre el barorreflejo en ratones adultos Sidestream cigarette smoke exposure effects on baroreflex in adult rats

    Directory of Open Access Journals (Sweden)

    Vitor E. Valenti

    2011-02-01

    N: Nuestros datos sugieren que tres semanas de exposición a la HLC no son suficientes para causar daño significativo a los parámetros cardiovasculares y sensibilidad barorreflexa en ratones Wistar normotensos.BACKGROUND: It has been evidenced in the literature that exposure to cigarette smoke causes hypertension in rats; however, it has not been demonstrated if the baroreflex function is impaired before the animal becomes hypertensive. OBJECTIVE: We evaluated short-term effects of sidestream cigarette smoke (SSCS exposure on baroreflex function in Wistar normotensive rats. METHODS: Rats were exposed to SSCS during three weeks, 180 minutes, five days per week, at a concentration of monoxide carbon between 100-300 ppm. Mean arterial pressure (MAP and heart rate (HR were evaluated through cannulation of the femoral vein and artery. RESULTS: There was no significant difference between control and SSCS groups regarding basal mean arterial pressure and heart rate, sympathetic and parasympathetic components of the baroreflex function. CONCLUSION: Our data suggest that three weeks of exposure to SSCS is not enough to significantly impair cardiovascular parameters and baroreflex sensitivity in normotensive Wistar rats.

  17. Transcrição reversa na determinação da expressão do mRNA para a enzima conversora de angiotensina testicular em animais tratados com zinco Assessment of the reverse transcriptase polymerase chain reaction technique in the determination of the mRNA expression for the testicular angiotensin-converting enzyme in zinc treated rats

    Directory of Open Access Journals (Sweden)

    Gilberto Simeone Henriques

    2005-12-01

    Full Text Available OBJETIVO: O objetivo deste trabalho foi otimizar as condições reacionais capazes de ocasionar variabilidade e de introduzir erros sistemáticos na reação em cadeia pela polimerase aplicada à análise da expressão gênica da isoforma testicular da enzima conversora de angiotensina. MÉTODOS: Avaliaram-se a concentração de cDNA, a concentração dos iniciadores, a temperatura de hibridização e o número de ciclos de desnaturação, hibridização e extensão. Para tanto, extraiu-se o RNA total por meio da reação com fenol-clorofórmio e isotiocianato de guanidina de amostras de testículos de ratos Wistar alimentados com uma ração contendo zinco. Em seguida, gerou-se o cDNA por transcrição reversa. Utilizando-se iniciadores específicos, amplificaram-se o gene de interesse (isoforma testicular da enzima conversora de angiotensina e o gene controle Gliceraldeído-3-Fosfato-Desidrogenase. As amostras foram então aplicadas em gel de agarose e submetidas à eletroforese, coradas em brometo de etídio e visualizadas sob luz ultravioleta. RESULTADOS: Demonstrou-se que a melhor condição reacional para a reação em cadeia pela polimerase da isoforma testicular da enzima conversora de angiotensina e do Gliceraldeído-3-Fosfato-Desidrogenase foi: (1 concentração inicial de cDNA de 2µg, (2 concentração de iniciadores de 200nM, (3 temperatura de hibridização entre 57,5ºC e 60,1ºC e (4 33 ciclos. CONCLUSÃO: Com essa otimização pôde-se minimizar as interferências sobre a técnica, contribuindo-se para a obtenção de dados comparativos a respeito da expressão gênica da enzima conversora de angiotensina testicular.OBJETIVE: The aim of the present work was to optimize the reaction conditions capable of generating variability and introducing systematic errors in the chain reaction of the polymerase used to analyze the gene expression for the testicular isoform of the angiotensin-converting enzyme. METHODS:The cDNA concentration

  18. Imobilização prolongada e remobilização da articulação fêmoro-tíbio-patelar de ratos: estudo clínico e microscópico Experimental immobilization and remobilization rat knee joints: clinical and microscopic study

    Directory of Open Access Journals (Sweden)

    R.J. Del Carlo

    2007-04-01

    Full Text Available Trinta e quatro ratos foram alocados em quatro grupos experimentais: sem imobilização (G1, com imobilização do joelho direito por 45 dias (G2, com imobilização e remobilização com atividade livre por cinco semanas (G3, imobilização e remobilização com atividade livre e natação por cinco semanas (G4. A imobilização interferiu negativamente na marcha e amplitude articular e o G4 apresentou melhor evolução na marcha nos cinco primeiros dias, em relação ao G3. Após esse período, a evolução foi similar. Os componentes do G2 apresentaram rigidez articular, não observada em G3 e G4. Histologicamente, a imobilização promoveu aumento da espessura da cápsula articular, evidenciada pela presença do tecido conjuntivo fibroso que substituiu o tecido adiposo no G2, mas em menor proporção em G3 e G4. A imobilização determinou perda de proteoglicanos da matriz cartilaginosa, aumento do número de condrócitos, dispostos de forma irregular, aumento da espessura da cartilagem calcificada, irregularidade da superfície articular, proliferação de tecido conjuntivo no espaço intra-articular e aumento da espessura do osso subcondral. O G3 apresentou maior número de alterações na cartilagem e osso subcondral, quando comparado com G4. A imobilização degenerou as células sinoviais, indicando diminuição da produção de fluido sinovial e do suprimento nutricional à cartilagem. Tanto a atividade livre quanto sua associação com a natação favoreceram o retorno das condições biomecânicas e da cápsula articular, anteriores à imobilização.Thirty-four rats were randomly allocated into one of four experimental groups: without immobilization (G1, immobilization of the right knee joint for 45 days (G2, immobilization and remobilization with free activity for 5 weeks (G3, and immobilization and remobilization with free activity and swimming program for 5 weeks (G4. The immobilization was prejudicial to march and flexibility

  19. Age-dependent changes in 24-hour rhythms of catecholamine content and turnover in hypothalamus, corpus striatum and pituitary gland of rats injected with Freund's adjuvant

    Directory of Open Access Journals (Sweden)

    Reyes Toso Carlos A

    2001-11-01

    Full Text Available Abstract Background Little information is available on the circadian sequela of an immune challenge in the brain of aged rats. To assess them, we studied 24-hour rhythms in hypothalamic and striatal norepinephrine (NE content, hypothalamic and striatal dopamine (DA turnover and hypophysial NE and DA content, in young (2 months and aged (18–20 months rats killed at 6 different time intervals, on day 18th after Freund's adjuvant or adjuvant's vehicle administration. Results Aging decreased anterior and medial hypothalamic NE content, medial and posterior hypothalamic DA turnover, and striatal NE concentration and DA turnover. Aging also decreased NE and DA content in pituitary neurointermediate lobe and augmented DA content in the anterior pituitary lobe. Immunization by Freund's adjuvant injection caused: (i reduction of DA turnover in anterior hypothalamus and corpus striatum; (ii acrophase delay of medial hypothalamic DA turnover in old rats, and of striatal NE content in young rats; (iii abolition of 24-h rhythm in NE and DA content of neurointermediate pituitary lobe, and in DA content of anterior lobe, of old rats. Conclusions The decline in catecholamine neurotransmission with aging could contribute to the decrease of gonadotropin and increase of prolactin release reported in similar groups of rats. Some circadian responses to immunization, e.g. suppression of 24-h rhythms of neurointermediate lobe NE and DA and of anterior lobe DA were seen only in aged rats.

  20. Histological analysis of low-intensity laser therapy effects in peripheral nerve regeneration in Wistar rats Avaliação histológica dos efeitos da laserterapia de baixa potência sobre os processos de regeneração nervosa periférica em ratos Wistar

    Directory of Open Access Journals (Sweden)

    Cibele Nazaré da Silva Câmara

    2011-02-01

    Full Text Available Purpose: Analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration of rats submitted to controlled crush through histological analysis. Methods: Were used 20 Wistar rats, to analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration, where the injury of the type axonotmesis was induced by a haemostatic clamp Crile (2nd level of the rack. The animals were randomly distributed in 2 groups. Control group (CG n = 10 and Laser group (LG n = 10. These were subdivided in 2 subgroups each, according to the euthanasia period: (CG14 _ n = 5 and CG21 _ n = 5 and (LG14 _ n = 5 and LG21 _ n = 5. At the end of treatment, the samples were removed and prepared for histological analysis, where were analyzed and quantified the following findings: Schwann cells, myelinic axons with large diameter and neurons. Results: In the groups submitted to low-intensity laser therapy, were observed an increase in the number of all analyzed aspects with significance level. Conclusion: The irradiation with low intensity laser (904nm influenced positively the regeneration of the sciatic nerve in Wistar rats after being injured by crush (axonotmesis, becoming the nerve recovery more rapid and efficient.Objetivo: Verificar a influência da terapia com laser de baixa potência na regeneração histológica do nervo ciático de ratos submetidos à neuropraxia controlada. Métodos: Foi utilizada a amostra de 20 ratos da linhagem Wistar, para verificar a influência da terapia com laser de baixa intensidade na regeneração nervosa periférica, onde a lesão do tipo axoniotmese foi induzida por meio de preensão com pinça hemostática de Crile. Os animais foram distribuídos randomicamente dois grupos. Grupo controle (CG n = 10, e Grupo laser (LG n = 10. Cada um destes grupos foi subdividido em dois subgrupos dependendo do período da eutanásia: (CG14 - n = 5 e CG21 - n = 5 e (LG14 - n = 5 e LG21 - n = 5. Ao final do

  1. Efeitos da prostaglandina E1 (PGE1 na gênese de capilares sanguíneos em músculo esquelético isquêmico de ratos: estudo histológico Effects of prostaglandin E1 (PGE1 in the genesis of blood capillaries in rat ischemic skeletal muscle: histological study

    Directory of Open Access Journals (Sweden)

    Dorival Moreschi Jr.

    2007-12-01

    Full Text Available CONTEXTO: A angiogênese terapêutica é uma modalidade de tratamento para pacientes com insuficiência arterial crônica que não têm indicação para revascularização direta ou angioplastia e que não tiveram uma resposta satisfatória ao tratamento clínico. Entre as drogas utilizadas para essa finalidade está a prostaglandina E1 (PGE1. OBJETIVO: Estudar os aspectos morfológicos na gênese de capilares sanguíneos em músculo esquelético do membro caudal de ratos submetidos à isquemia sob a ação da PGE1, administrada por via intramuscular (IM ou endovenosa (EV. MÉTODOS: Foram utilizados 48 ratos, linhagem Wistar-UEM, distribuídos aleatoriamente em três grupos de 16, redistribuídos igualmente em dois subgrupos, observados no 7º e 14º dias, sendo um grupo controle onde apenas foi provocada a isquemia no membro, outro com a isquemia e a injeção da PGE1 via IM e outro com a isquemia e a injeção da PGE1 EV. Para análise dos resultados, foram realizadas a coloração com hematoxilina e eosina (HE e coloração imuno-histoquímica. RESULTADOS: Constatou-se um aumento estatisticamente significativo no número de capilares nos subgrupos com o uso da PGE1 IM e EV, através da contagem nos cortes corados com HE. A imunomarcação não foi eficiente para a quantificação dos capilares. CONCLUSÕES: A PGE1, administrada por via IM ou EV, promoveu, após 14 dias de observação, um aumento no número de capilares no músculo esquelético de ratos submetido à isquemia, identificáveis histologicamente com a coloração em HE. A imunocoloração não permitiu estabelecer uma correlação com o aumento de vasos encontrados na coloração com HE.BACKGROUND: Therapeutic angiogenesis is a treatment modality for patients with chronic arterial insufficiency who do not have indication for direct reconstruction or angioplasty and who were not successfully submitted to clinical treatment. Prostaglandin E1 (PGE1 is one of the drugs used for this

  2. Efeito da exposição à fumaça de cigarro durante a prenhez e a lactação de ratas e sua prole sobre parâmetros séricos e morfométricos Effect of cigarette smoke exposure during pregnancy and lactation of rats and the offspring on the serum and morphometric parameters

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    Patrícia Rodrigues Lourenço Gomes

    2010-12-01

    Full Text Available OBJETIVO: investigar o efeito da exposição à fumaça de cigarro durante os períodos de prenhez e lactação de ratas sobre o ganho de peso corpóreo e tecidual, parâmetros séricos e produção láctea, bem como a repercussão na prole, desde o nascimento até o período jovem adulto. MÉTODOS: 40 ratas Wistar prenhes foram divididas em quatro grupos: CG - não expostas à fumaça de cigarro e sacrificadas ao término da gestação; CL - não expostas à fumaça de cigarro e sacrificadas ao término da lactação; FG - expostas à fumaça de cigarro e sacrificadas ao término da gestação; FL - expostas à fumaça de cigarro e sacrificadas ao término da lactação. As proles foram separadas por sexo e dividas conforme o grupo de suas mães, sendo sacrificadas na fase jovem adulto. Nas ratas e nas proles foram avaliados peso tecidual, peso corpóreo e parâmetros séricos. Foi também analisada a produção láctea por filhote. RESULTADOS: o peso corpóreo de ratas estava diminuído no grupo FL durante a lactação (CL=267,0±7,2; FL=235,5±7,2 g*,*pPURPOSE: to investigate the effect of cigarette smoke exposure on body and tissue weight gain, serum parameters and milk yield during pregnancy and lactation in rats, and the impact on offspring from birth toil young adulthood. METHODS: 40 Wistar pregnant rats were randomly divided into: CG - not exposed to cigarette smoke and sacrificed at the end of pregnancy; CL - not exposed to cigarette smoke and sacrificed at the end of lactation; FG - exposed to cigarette smoke and sacrificed at the end of pregnancy; FL - exposed to cigarette smoke and sacrificed at the end of lactation. The offspring were separated by gender and divided according to their mothers' groups. Tissue weight, body weight and serum parameters were evaluated in rats and offspring. Milk yield per pup was calculated. RESULTS: body weight was decreased in FL during lactation (CL=267.0±7.2; FL=235.5±7.2 g*,*p<0.05. Adipose tissue

  3. Influência da glândula pineal sobre a ingestão de água e NaCl em ratas normais e ooforectomizadas Influence of oophorectomy and pinealectomy on water and NaCl ingestion by adult rats

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    José Maria Soares Jr.

    1999-07-01

    Full Text Available Objetivo: avaliar a influência da pineal sobre a ingestão de água e de NaCl em ratas normais e ooforectomizadas. Métodos: utilizaram-se 48 (n = 48 ratas adultas, divididas ao acaso em quatro grupos: GI - mantidas sem manipulação, servindo como controle (n = 20; GII - submetidas à ooforectomia bilateral (n = 8; GIII - submetidas à pinealectomia (n = 12; GIV - submetidas à ooforectomia bilateral e à pinealectomia (n = 8. A seguir, todos os animais foram mantidos em gaiolas individuais; após três semanas. Determinou-se diariamente a fase do ciclo por esfregaço vaginal e anotou-se diariamente o volume ingerido de água e NaCl a 0,25 M, por aproximadamente três meses. Resultados: 1 as ratas submetidas somente à pinealectomia apresentaram maior freqüência da fase de estro, sendo que algumas entraram em estado de estro permanente; 2 o consumo de líquidos (água e solução salina não se alterou durante as diferentes fases do ciclo estral; 3 as ratas do grupo submetido à ooforectomia tiveram maior consumo de água, sendo que a pinealectomia nesses animais restabeleceu o consumo normal de água; 4 os animais ooforectomizados e os ooforectomizados e pinealectomizados mostraram redução do consumo médio de solução salina. Conclusões: os dados obtidos sugerem que a glândula pineal poderia modular a ação dos esteróides ovarianos sobre a ingestão de sal e água em ratas adultas.Purpose: to evaluate the effects of oophorectomy and pinealectomy on the ingestion of water and NaCl solution by adults female rats. Methods: forty-eight adult virgin female rats (Wistar EPM 1 weighing 200 g were kept on routine laboratory care and fed water and Purina rat chow ad libitum. The animals were random by divided into four groups: GI - maintained without manipulation as a control group (n = 20; GII - submitted to bilateral oophorectomy (n = 8; GIII - submitted to pinealectomy (n = 12; GIV - submitted to bilateral oophorectomy and pinealectomy (n

  4. Glutamine or whey-protein supplementation on alloxan-induced diabetic rats. Effects on CD4+ and CD8+ lymphocytes Efeitos da oferta de glutamina ou de proteína do soro de leite sobre os linfócitos CD4+ e CD8+ em ratos diabéticos aloxano induzidos

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    Renato Motta Neto

    2007-06-01

    Full Text Available PURPOSE: To evaluate the effects of glutamine (L-Gln or whey-protein supplementation on CD4+ and CD8+ lymphocytes in alloxan-induced diabetic rats. METHODS: Thirty-two healthy male Wistar rats were used in the experiment. Eight rats served as baseline controls (G-1. The remaining 24 animals received alloxan 150mg/Kg intraperitonially dissolved in buffer solution and were equally distributed in 3 subgroups, upon induction of diabetes mellitus, and treated as follows: (G2: saline, 2.0ml; (G3: glutamine solution (0.7g/kg, 2.0 ml; and (G4: whey-protein (WPS solution (0.7g/kg, 2.0 ml. All solutions were administered by daily 7:00 AM gavages during 30 days. Next, arterial blood samples (3.0 ml were collected from anesthetized rats for CD4+ and CD8+ lymphocyte count through flow cytometry technology. RESULTS: CD4+ and CD8+ counts decreased significantly in all groups compared with baseline values (G1. G2 rats CD4+/CD8+ ratio decreased significantly compared with G1. CD4+/CD8+ ratio increased significantly (>260% in L-Gln treated group (G3 compared with saline-treated rats (G2. There were no statistical differences in lymphocyte counts (CD4+ and CD8+ between L-Gln (G3 and saline-treated (G2 groups. There was a significant reduction in CD8+ cell count compared with CD4+ cell count in L-Gln treated rats (G3. CONCLUSION: The offer of L-Gln to experimental diabetic rats enhances the immunologic response to infection.OBJETIVO: Avaliar os efeitos da suplementação de glutamina ou proteína do soro de leite ( PSL sobre os linfócitos CD4+ e CD8+ em ratos diabéticos aloxano induzidos. MÉTODOS: Trinta e dois ratos Wistar machos, saudáveis, foram utilizados no estudo. Oito ratos foram usados como controles basais (G1. Os 24 animais remanescentes foram equitativamente distribuídos em 3 subgrupos, após indução do diabetes mellitus por injeção intraperitonial de aloxano (150mg/Kg e tratados como se segue: (G2: salina; (G3: 2,0 ml de solução de glutamina

  5. Altered neuronal activity in the primary motor cortex and globus pallidus after dopamine depletion in rats.

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    Wang, Min; Li, Min; Geng, Xiwen; Song, Zhimin; Albers, H Elliott; Yang, Maoquan; Zhang, Xiao; Xie, Jinlu; Qu, Qingyang; He, Tingting

    2015-01-15

    The involvement of dopamine (DA) neuron loss in the etiology of Parkinson's disease has been well documented. The neural mechanisms underlying the effects of DA loss and the resultant motor dysfunction remain unknown. To gain insights into how loss of DA disrupts the electrical processes in the cortico-subcortical network, the present study explores the effects of DA neuron depletion on electrical activity in the primary motor cortex (M1), on the external and the internal segment of the globus pallidus (GPe and GPi respectively), and on their temporal relationships. Comparison of local field potentials (LFPs) in these brain regions from unilateral hemispheric DA neuron depleted rats and neurologically intact rats revealed that the spectrum power of LFPs in 12-70Hz (for M1, and GPe) and in 25-40Hz (for GPi) was significantly greater in the DA depleted rats than that in the control group. These changes were associated with a shortening of latency in LFP activities between M1 and GPe, from several hundred milliseconds in the intact animals to close to zero in the DA depleted animals. LFP oscillations in M1 were significantly more synchronized with those in GPe in the DA depleted rats compared with those in the control rats. By contrast, the synchronization of oscillation in LFP activities between M1 and GPi did not differ between the DA depleted and intact rats. Not surprisingly, rats that had DA neuron depletion spent more time along the ladder compared with the control rats. These data suggest that enhanced oscillatory activity and increased synchronization of LFPs may contribute to movement impairment in the rat model of Parkinson's disease. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. Electric microcurrent in the restoration of the skin undergone a trichloroacetic acid peeling in rats Microcorrente elétrica na restauração da pele submetidos ao peeling de ácido tricloroacético em ratos

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    Vilma Natividade Silva Santos

    2004-10-01

    Full Text Available PURPOSE: To investigate the influence of neuromuscular electric stimulation by microcurrent (MENS - Microcurrent Electric Neuromuscular Stimulation in the restoration of the skin of rats undergone trichloroacetic acid (TCA. METHODS: Thirty-two hairless, male, adult rats were divided into two groups (with 16 animals each. In the Control Group (G1 the application of microcurrent was simulated 48 hours after the peeling. In the Experimental Group (G2 MENS was applied 48 hours after the TCA peeling, each 48 hours up to the Day 21, under the frequency of 0.5 Hz, intensity of 50mu A, and lasting 20 minutes. The area of the lesion was evaluated on Days 7, 14, and 21 after-peeling by the Paper Template Method, and on Day 21 the histological analysis was performed. RESULTS: On microscopy changes were seen in the fibroblasts amount and structure, and in the collagen of the experimental group, with statistically significant difference (pOBJETIVO: Investigar a influência da estimulação elétrica neuromuscular por microcorrente (MENS - Microcurrent Electric Neuromuscular Stimulation na restauração da pele de ratos submetidos ao peeling com ATA (ácido tricloroacético. MÉTODOS: Foram utilizados 32 ratos hair less machos adultos, separados em dois grupos (com 16 animais cada.No Grupo Controle (G1 foi simulada a aplicação da microcorrente 48 horas após o peeling. No Grupo Experimental (G2 a MENS foi aplicada 48 horas após o peeling com ATA, realizada, a cada 48 horas, até o 21º dia, com freqüência de 0,5 Hz, intensidade de 50mi A e duração de 20 minutos. Avaliou-se a área da lesão no 7º ,14º e 21º dia pós-peeling, com o Método Gabarito de Papel, e no 21º dia foi realizada a análise histológica. RESULTADOS: Na microscopia foram observadas modificações na quantidade e na estrutura dos fibroblastos, e no colágeno do Grupo Experimental em relação ao Grupo Controle. Pelo Método Gabarito de Papel, observou-se redução do tempo para a

  7. Avaliação do efeito do extrato hidroalcoólico de Schinus terebinthifolius Raddi (Aroeira no processo de cicatrização da linea alba de ratos Evaluation of the hydro-alcoholic Schinus terebinthifolius Raddi (Aroeira extract in the healing process of the alba linea in rats

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    José Aldemir Teixeira Nunes Jr.

    2006-01-01

    sutura. Notou-se diferença significativa para proliferação fibroblástica (p=0,014 na avaliação intergrupo de três dias, e para inflamação crônica (p=0,023 e reação gigantocelular do tipo corpo estranho (p=0,008 na avaliação intergrupo de sete dias. Na análise intragrupo controle, houve diferença significativa para inflamação crônica no subgrupo três dias, e, finalmente, na análise intragrupo experimento, observou-se diferença significativa para inflamação aguda e proliferação de fibroblastos (p=0,001 e p=0,020 no subgrupo de três dias em relação ao subgrupo de sete. CONCLUSÃO: A injeção intraperitoneal do extrato hidroalcoólico de Schinus terebinthifolius Raddi em laparotomias medianas de ratos não alterou a cicatrização na análise macroscópica e induziu a aumento da carga máxima de ruptura e deformação máxima da linha alba na análise tensiométrica. Na análise histológica, determinou efeito cicatrizante no subgrupo de animais experimento de três dias.PURPOSE: To evaluate the healing process in the abdominal wall of rats after the intra-peritoneal injection of the hydro-alcoholic Schinus terebinthifolius Raddi extract. METHODS: Forty Wistar rats were used, distributed in two groups of 20 animals, divided into two subgroups, according to the death day, three or seven, after the intra-peritoneal injection of the extract. The experimental group was injected with only one dose of the hydro-alcoholic Aroeira extract (100mg per animal kilogram; the control group was injected with only one dose of isotonic saline solution at 0.9%. After the animal death, an inventory of the peritoneal cavity was carried out in a careful search for any adhesion, followed by the resection of the anterior abdominal wall encompassing the operating incision in order to examine it for evidence of infection. The adhesions were classified according to the Nair criteria. The tensiometric assessment was performed by means of the measurement of the maximum

  8. Avaliação da integração de enxerto de pele com a utilização do 2-octilcianoacrilato: estudo experimental em ratos Evaluation of skin graft integration using 2-octil-cyanocrylate: experimental study in rats

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    Gilvani Azor de Oliveira e Cruz

    2000-01-01

    Full Text Available A fixação dos enxertos de pele total através da sutura demanda tempo cirúrgico relativamente longo. O surgimento de adesivos teciduais tem se apresentado com várias aplicações clínicas, constituindo alternativa à sutura convencional. O objetivo deste estudo é avaliar a eficácia do adesivo 2-octilcianoacrilato, quando comparado à sutura simples, na fixação de enxertos de pele total, analisando-se a integração do enxerto. Foram retirados enxertos de pele total de 1 cm², bilateralmente, do dorso de nove ratos machos, com peso médio de 150 gramas. A pele retirada para a enxertia foi recolocada sobre o defeito, a fixação foi realizada através de sutura contínua com fio monofilamentar de náilon 4-0 no lado esquerdo e adesivo de 2-octilcianoacrilato no lado direito. Os aspectos clínico e histológico da integração do enxerto foram analisados no 7º e 14º dia de pós operatório. Utilizou-se o software Sens-a-Ray para comparação de resultados. Evidenciamos maior integração do enxerto de pele total no lado do adesivo de 2-octilcianoacrilato (80% em comparação ao lado da sutura contínua (44%, sendo que houve perda total do enxerto do lado da cola de 2-octilcianoacrilato em 11,1% do casos comparado a 33,3% da sutura contínua. O tempo médio da fixação do enxerto no grupo da cola de 2-octilcianoacrilato foi de 23 segundos, contra 4 minutos da sutura convencional. Foi demonstrado uma maior integração e um menor tempo de fixação do enxerto no grupo do adesivo 2- octilcianoacrilato.Fixation of full thickness skin grafts by suture takes a long operative time. The development of tissue adhesives became an alternative for conventional suture. The aim of this study is to evaluate the efficacy of octyl-2-cyanocrylate in graft integration, compared to conventional suture in full thickness skin grafting. Two 1 cm² skin grafts were obtained billaterally from the back of 9 male rats, and then fixated over the defect, with nylon

  9. Effects of topical 0.2% Cyclosporine A on corneal neovascularization induced by xenologous amniotic membrane implantation into a corneal stroma micropocket of rats Efeitos do uso tópico da Ciclosporina A (CsA 0,2% na neovascularização corneal induzida pelo implante de membrana amniótica xenógena em microbolsa no estroma da córnea de ratos

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    Juliana F. Milani

    2008-08-01

    Full Text Available The objective of the study was to evaluate the topical effects of 0.2% Cyclosporine A (CsA on corneal neovascularization of rats following surgical implantation of equine amniotic membrane into a corneal stroma micropocket. The implantation of xenologous amniotic membrane was performed bilaterally in 90 rats. In the same day of the surgery each right eye started receiving topical CsA twice a day. The left eye received no medication and served as a control. The evaluation of corneal neovascularization was performed by computerized image analysis and histopathological evaluation at 1, 3, 7, 15, 30 and 60 days postoperatively. For the image analysis 10 animals were used per time period, and for the histopathological examination, five animals were used per time period. Image analysis found that corneal neovascularization began on the 3rd postoperative day, reached its peak on the 7th day, and then progressively and rapidly decreased. Statistic analysis indicated that neovascularization of the CsA treated eye on the 7th day was significantly higher than that observed in untreated eyes. On the 30th day, however, this pattern was reversed with the neovascularization observed in the CsA treated eyes declining to the low levels observed on the 3rd day. The degree of neovascularization in the untreated eyes on the 30th day declined to the baseline levels found on day 3 at the 60th day. Histopathological analysis indicated that deposition of collagen in the implanted tissue was completed by the 15th day. Therefore, we concluded that (1 equine amniotic membrane in rat corneal stroma produced an intense neovascularization until the 15th day postoperatively and then regressed, (2 deposition of collagen of the implanted tissue was completed on the 15th day postoperatively, and (3 use of CsA was associated with increase in the corneal neovascularization initially, followed by a quick and intense regression.Este estudo teve como objetivo a avaliação dos

  10. Valores de referência de alguns parâmetros fisiológicos de ratos do Biotério Central da Universidade Estadual de Maringá, Estado do Paraná = Reference values of some physiological parameters in the rats of the Central Biotery at the State University of Maringá, State of Paraná

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    Jailson Araújo Dantas

    2006-07-01

    Full Text Available O objetivo deste trabalho foi estabelecer valores de referência de alguns parâmetros fisiológicos (bioquímicos, hematológicos e urinários de ratos provenientes do Biotério Central da Universidade Estadual de Maringá, Estado do Paraná. Foram utilizados ratos machos, normais e saudáveis da linhagem Wistar, com peso de 200 a 220 g. Foiavaliado o perfil hematológico (leucócitos: totais, mononucleares e polimorfonucleares e a determinação bioquímica de vários constituintes plasmáticos (aspartato aminotransferase, alanina aminotransferase, glicose, creatinina, fosfatase alcalina, fosfatase ácida, uréia, colesterol total, colesterol HDL, triglicerídeos, teste de tolerância à glicose GTT e urinários (glicose, creatinina. É imprescindível que cada laboratório estabeleça seu conjunto devalores de referência dos animais normais, de acordo com a dieta, a linhagem, o sexo e a idade de cada espécie utilizada. Assim, através deste trabalho, o pesquisador pode identificar desvios dos parâmetros, facilitando a escolha dos animais para o estudo.The present work was carried out to establish reference values for some physiological parameters (biochemical, hematological and urinary in rats proceeding from the Central Biotery at the State University of Maringá, State of Paraná. Normal male Wistar rats weighing 200-220 g were used. The hematological profile (total leukocytes, mononuclear and polymorphonuclear cells count, theclinic chemistry (aspartate aminotransferase, alanine aminotransferase, glucose, creatinine, alkaline phosphatase, acid phosphatase, urea, cholesterol, HDL-c, triglycerides, glucose tolerance and urinary parameters (glucose and creatinine were determined. Each laboratory should establish a database of reference values for their animals, according to diet, strain, sex and age foreach species used. Thus, in this work, a database is provided regarding reference values of some physiological parameters in rats for use

  11. Efeitos da suplementação do 17beta-estradiol no dano oxidativo cardíaco de ratas submetidas à privação dos hormônios ovarianos Effects of 17beta-estradiol replacement on cardiac oxidative damage in rats submitted to ovarian hormone deprivation

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    Sônia Maria Rolim Rosa Lima

    2007-01-01

    Full Text Available OBJETIVO: avaliar o estresse oxidativo em tecido cardíaco de ratas ooforectomizadas, com ou sem terapia hormonal. MÉTODOS: ratas Wistar foram divididas em três grupos: grupo controle (GC, grupo ooforectomizada (GO e grupo ooforectomizada + suplementação hormonal (GOS. A privação estrogênica foi obtida pela ooforectomia bilateral. Uma semana após a ooforectomia, um pellet de 1,5 mg de 17beta-estradiol foi implantado nos animais do grupo GOS. Nove semanas após a ooforectomia, o tecido cardíaco foi obtido para a análise do estresse oxidativo por meio da medida da quimiluminescência e da atividade das enzimas antioxidantes catalase (CAT, superóxido dismutase (SOD e glutationa peroxidase (GPx. RESULTADOS: a quimiluminescência estava aumentada no GO (7348±312 cps/mg proteína quando comparado ao GC (6250±41 cps/mg proteína, pPURPOSE: to evaluate oxidative stress in cardiac tissue of ovariectomized rats, with and without hormonal therapy. METHODS: female Wistar rats were divided in three groups: control group (CG; ovariectomized group (OG; ovariectomized group with estrogen supplementation (ESG. The estrogen deprivation was done through bilateral ovariectomy. After one week from the ovariectomy, a pellet of 1.5 mg of 17beta-estradiol was implanted in the ESG animals. Nine weeks after the ovariectomy, cardiac tissue was obtained for the analysis of the oxidative stress through CL (chemiluminescence, and measurement of antioxidant enzymes catalase (CAT, superoxide dismutase (SOD and gluthatione peroxidase (GPx. RESULTS: CL was increased in the OG (7348±312 cps/mg protein when compared with the CG (6250±41 cps/mg protein, p<0.01, but there was no significant difference between the CG and the ESG (6170±237 cps/mg protein. Ovariectomy reduced SOD (35%, p<0.05 and CAT (22%, p<0.001 activities in the OG as compared with the CG. Hormonal therapy normalized antioxidant enzymes activities in the ESG. There was no statistically significant

  12. The effect of gastric dilatation in rats submitted to gasified water ingestion under the hepatic metabolic function Efeito da dilatação gástrica em ratos submetidos à ingestão de água gaseificada sobre a função metabólica hepática

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    José Roberto Ferreira Santiago

    2008-10-01

    Full Text Available INTRODUCTION: The amounts of people that are overweight have been increasing within the population in significant ways during the last decades. In this view, gasified beverages have become an important environmental concern in relation to the eating habits of people, especially who lives in the USA, Mexico, and Brazil. In this order, these three countries constitute the major beverages producers and consumers of the whole world. PURPOSE: To investigate the effects of gastric dilatation in rats submitted to gasified water ingestion, uniform vehicle for all soft drinks, under metabolic patterns of the hepatic function. METHODS: Two groups of 15 rats were formed and observed during two weeks. The rats of the group I, were fed with 200g/day of rat food ad libitum and 100ml of non-gasified water during three daily periods. The rats composing the group II, were fed with 200g/day of rat food ad libitum and 100ml of gasified water within 3 daily periods. The media (x and standard deviation (s were calculated through the paired t-test for each group in order to compare the effects of the different types of water and its effect in each one of them. RESULTS: The results indicated that the animals which were submitted to the treatment with gasified water (G-II, presented an increase of glutamic-pyruvic transaminase (GPT and alkaline phosphatase (ALP (pp>0,05 and increase of the gastric area with macroscopic morphologic alterations, such as the loss of the characteristic linear depressions on the surface of the mucous membrane. CONCLUSION: The gasified water favored the expansion of the gastric area and contributed to the extinction of the linear depressions of the mucous organ, which caused metabolic alterations of the hepatic function.INTRODUÇÃO: O excesso de peso na população aumentou de forma significante nas últimas décadas e as bebidas gasosas tornaram-se um fator ambiental importante no comportamento alimentar das pessoas, sendo os EUA, M

  13. Ação da Betametasona em Ratas Prenhes: Impacto sobre os Níveis de Corticosterona e Glândulas Adrenais Maternas e Fetais Effect of Betamethasone on Pregnant Rats: Impact on Corticosterone Level and Maternal and Fetal Adrenal Glands

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    Eduardo de Souza

    2001-12-01

    Full Text Available Objetivo: a utilização repetitiva do corticóide antenatal objetivando acelerar a maturidade pulmonar fetal tem sido muito empregada no risco de parto prematuro, o que nos motivou a estudar a dosagem de corticosterona no termo e aspectos morfológicos das glândulas adrenais maternas e fetais de ratas albinas submetidas à ação da betametasona na segunda metade da prenhez, para verificar conseqüências dessa terapêutica. Métodos: utilizamos 30 ratas prenhes, distribuídas em 3 grupos numericamente iguais. As do Grupo I receberam betametasona nos dias 11, 12, 18 e 19 da prenhez. As do Grupo II receberam água destilada nesses dias (grupo controle, e as do Grupo III não receberam qualquer medicamento, constituindo grupo controle de estresse. Foram todas sacrificadas no 20º dia de prenhez, quando dosamos a corticosterona no sangue das matrizes e extirpamos as glândulas adrenais maternas e fetais para exame de microscopia óptica. Resultados: a dosagem de corticosterona plasmática foi significantemente menor no grupo tratado com betametasona (4,8 mg/dL, quando comparada aos grupos controles (17,7 e 26,8 mg/dL. À microscopia óptica observou-se intensa vacuolização citoplasmática na zona fasciculada das adrenais maternas e fetais no grupo que utilizou a betametasona, indicando intensa supressão adrenal secundária ao uso do medicamento. Conclusões: o uso repetitivo e prolongado de corticóides, em ratas prenhes, para acelerar a maturidade pulmonar fetal determina supressão adrenal materna e fetal.Purpose: the repetitive use of antenatal corticosteroid therapy for acceleration of fetal lung maturation has been common in cases at risk of preterm delivery. We studied the corticosterone levels at term and the morphologic aspects in the maternal and fetal adrenal glands submitted to the effect of betamethasone in the second half of rat pregnancy in order to verify its consequences. Methods: thirty female pregnant rats were divided into

  14. Efeito da farinha de semente de abóbora (Cucurbita maxima, L. sobre o metabolismo glicídico e lipídico em ratos The pumpkin (Cucurbita maxima, L. seed flour effect on the rat glucose and lipid metabolism

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    Priscila Machado de Cerqueira

    2008-04-01

    Full Text Available OBJETIVO: O objetivo desta pesquisa foi avaliar o efeito da farinha de semente de abóbora (Cucurbita maxima, L. sobre o metabolismo glicídico e lipídico em ratos. MÉTODOS: Vinte ratos Wistar machos recém-desmamados, distribuídos em quatro grupos, receberam por 10 dias, rações controle e experimental com farinhas de semente de abóbora integral, peneirada ou residual substituindo 30% do valor total de amido e dextrina da dieta controle. As dietas foram isocalóricas. Foram determinados os macronutrientes e a fibra insolúvel nas farinhas. As dietas tiveram a composição química calculada a partir dos dados dos rótulos dos produtos, da tabela de composição de alimentos e da análise química das farinhas de semente de abóbora. O peso corporal e a ingestão dos animais foram tomados a cada 48 horas. O sangue, coletado por punção cardíaca, teve os níveis de triacilgliceróis, colesterol e glicose analisados por métodos enzimáticos. RESULTADOS: As farinhas de semente de abóbora foram boas fontes de proteínas, lipídeos e, especialmente, fibras alimentares. Os animais tiveram ganho ponderal e ingestão semelhante (p>0,05. Os níveis de glicose e triacilgliceróis foram reduzidos significantemente para os grupos que receberam dietas com farinhas de semente de abóbora integral e peneirada. CONCLUSÃO: Frente às suas propriedades química e funcional, concluiu-se que a farinha de semente de abóbora interferiu no metabolismo do rato diminuindo significantemente os níveis de glicose e triacilgliceróis séricos.OBJECTIVE: The aim of the present research was to evaluate the pumpkin (Cucurbita maxima, L. seed flour effect on the rat glucose and lipid metabolism. METHODS: Twenty recent weaned male Wistar rats, divided in four groups, received for 10 days control and experimental diets containing whole, sifted and residual pumpkin seed flour on the rate of 30% of the total starch and dextrin in the control diet. All diets were

  15. Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations Estudo da densidade das células ganglionares no intestino terminal de ratos portadores de anomalia anorretal

    Directory of Open Access Journals (Sweden)

    Maurício Macedo

    2007-12-01

    Full Text Available PURPOSE: To study the ganglion cells (GC in the terminal bowel of rats with ethylenethiourea (ETU induced anorectal malformations (ARM. METHODS: The animals were divided into three groups: Group A - normal fetuses from pregnant rats that were not administered ETU; Group B - fetuses without ARM born from pregnant rats that were administered ETU and Group C - fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses’ terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION: Ganglion cell densities are decreased in the terminal bowel of rats with ARM.OBJETIVO: Estudar as células ganglionares (CG no intestino terminal de ratos portadores de anomalia anorretal (AAR induzida pela etilenotiouréia (ETU. MÉTODOS: Os animais foram distribuídos em três grupos: Grupo A - fetos normais, obtidos de ratas grávidas às quais não foi administrada ETU; Grupo B - fetos não portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU e Grupo C - fetos portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU. A ETU foi administrada no décimo primeiro dia de gestação na dose de 125 mg/Kg, por gavagem. As ratas foram submetidas à laparotomia e histerotomia para retirada dos fetos no vigésimo primeiro dia de gestação. O intestino terminal dos fetos foi retirado e analisado por imunohistoquímica para pesquisa de CG. RESULTADOS: Foram encontradas diferenças estatisticamente significantes entre os grupos A, B e C quanto à densidade de CG. O grupo A apresentou a maior densidade

  16. Changes in Binding of [123I]CLINDE, a High-Affinity Translocator Protein 18 kDa (TSPO) Selective Radioligand in a Rat Model of Traumatic Brain Injury

    DEFF Research Database (Denmark)

    Donat, Cornelius K; Gaber, Khaled; Meixensberger, Jürgen

    2016-01-01

    , somatosensory and parietal cortex, as well as in the hippocampus and thalamus. Interestingly, binding was also significantly elevated in the contralateral M1 motor cortex following TBI. Craniotomy without TBI caused a less marked increase in [(123)I]CLINDE binding, restricted to the ipsilateral hemisphere...... studies using single-photon emission computed tomography to image the neuroinflammatory response after stroke. In this study, we used the same tracer in a rat model of TBI to determine changes in TSPO expression. Adult Sprague-Dawley rats were subjected to moderate controlled cortical impact injury...... and sacrificed at 6, 24, 72 h and 28 days post surgery. TSPO expression was assessed in brain sections employing [(123)I]CLINDE in vitro autoradiography. From 24 h to 28 days post surgery, injured animals exhibited a marked and time-dependent increase in [(123)I]CLINDE binding in the ipsilateral motor...

  17. Histological study on acute inflammatory reaction to polyurethane-coated silicone implants in rats Estudo histológico da reação inflamatória aguda ao implante de silicone revestido com poliuretano em ratos

    OpenAIRE

    Paulo Roberto da Silva Mendes; Jorge Bins-Ely; Eduardo Arnaut dos Santos Lima; Zulmar Antonio Accioli de Vasconcellos; Armando José d'Acampora; Rodrigo d' Eça Neves

    2008-01-01

    PURPOSE: Evaluating histologically the silicone peri-implant coated by polyurethane inflammation associated to the use of anti-microbial and bacterial contamination. METHODS: It was used 35 Wistar rats. The animals were divided in seven groups: I - Control; II - implant cavity contamination with10 bacteria/ml; III - implant cavity contamination with 10 bacteria/ml; IV - implant cavity contamination with 10 bacteria/ml; V - identical contamination to group II and implant immersions in anti-mic...

  18. Role of the vitamin C in diethylnitrosamine-induced esophageal cancer in Wistar rats O efeito inibidor da vitamina C na carcinogênese esofágica induzida pela dietilnitrosamina em ratos Wistar

    OpenAIRE

    Almino Cardoso Ramos; Marina Rachel Araujo; Luiz Roberto Lopes; Nelson Adami Andreollo

    2009-01-01

    PURPOSE: To evaluate the inhibitory effect of vitamin C on the experimental esophageal carcinogenesis induced by diethylnitrosamine (NDEA). METHODS: Sixty Wistar male rats aged three months, with mean weight of 210 g were employed in the study and were divided into four different groups according to the drinking drugs: group I - controls: only water, seven days a week; group II - only vitamin C, seven days a week; group III - NDEA, three days a week and water during the other four days; group...

  19. The rat with oxygen-induced retinopathy is myopic with low retinal dopamine.

    Science.gov (United States)

    Zhang, Nan; Favazza, Tara L; Baglieri, Anna Maria; Benador, Ilan Y; Noonan, Emily R; Fulton, Anne B; Hansen, Ronald M; Iuvone, P Michael; Akula, James D

    2013-12-19

    Dopamine (DA) is a neurotransmitter implicated both in modulating neural retinal signals and in eye growth. Therefore, it may participate in the pathogenesis of the most common clinical sequelae of retinopathy of prematurity (ROP), visual dysfunction and myopia. Paradoxically, in ROP myopia the eye is usually small. The eye of the rat with oxygen-induced retinopathy (OIR) is characterized by retinal dysfunction and short axial length. There have been several investigations of the early maturation of DA in rat retina, but little at older ages, and not in the OIR rat. Therefore, DA, retinal function, and refractive state were investigated in the OIR rat. In one set of rats, the development of dopaminergic (DAergic) networks was evaluated in retinal cross-sections from rats aged 14 to 120 days using antibodies against tyrosine hydroxylase (TH, the rate-limiting enzyme in the biosynthesis of DA). In another set of rats, retinoscopy was used to evaluate spherical equivalent (SE), electoretinography (ERG) was used to evaluate retinal function, and high-pressure liquid chromatography (HPLC) was used to evaluate retinal contents of DA, its precursor levodopamine (DOPA), and its primary metabolite 3,4-dihydroxyphenylacetic acid (DOPAC). The normally rapid postnatal ramification of DAergic neurons was disrupted in OIR rats. Retinoscopy revealed that OIR rats were relatively myopic. In the same eyes, ERG confirmed retinal dysfunction in OIR. HPLC of those eyes' retinae confirmed low DA. Regression analysis indicated that DA metabolism (evaluated by the ratio of DOPAC to DA) was an important additional predictor of myopia beyond OIR. The OIR rat is the first known animal model of myopia in which the eye is smaller than normal. Dopamine may modulate, or fail to modulate, neural activity in the OIR eye, and thus contribute to this peculiar myopia.

  20. Estudo experimental dos efeitos da heparina de baixo peso molecular (Enoxaparina na formação de calo ósseo em fêmures de ratos The effects of low-molecular-weight heparin (Enoxaparin on bony callus formation in rats' femurs - an experimental study

    Directory of Open Access Journals (Sweden)

    Salim Mussi Filho

    2006-01-01

    Full Text Available O tromboembolismo venoso é uma complicação grave que pode ocorrer após fraturas. O tratamento anticoagulante mais utilizado é com a heparina de baixo peso molecular (HBPM. Existem estudos que mostram que essa droga pode interferir no metabolismo ósseo. Com o objetivo de avaliar a influência da HBPM no processo de formação de calo ósseo, realizamos um estudo experimental em ratos. A amostra constituiu-se de 22 ratos de linhagem Wistar, machos, que foram submetidos à fratura diafisária de seus fêmures direitos. Foram divididos em dois grupos de 11. No grupo controle, os animais recebiam soro fisiológico e no grupo de estudo, recebiam HBPM, enoxaparina, diariamente, por 28 dias. Após este período os ratos foram submetidos à eutanásia e os fêmures foram avaliados. No estudo macroscópico foi constatada consolidação em 11 animais (100% que não receberam enoxaparina, e, em dez animais (90,9% que receberam a droga em estudo. No estudo histológico foi constatada a formação de calo ósseo em todos os fêmures. Concluiu-se neste experimento que a enoxaparina não altera o processo de consolidação óssea em fêmures de ratos Wistar.Venous thromboembolism is a serious complication that may follow fractures. The most commonly used anticoagulant treatment is low-molecular-weight heparin (LMWH. There are some studies showing that this drug may interfere on bone metabolism. With the objective of evaluating the LMWH influence on the process of bony callus formation, we conducted an experimental study on rats. Sample was constituted of 22 Wistar male rats, which were submitted to diaphyseal fracture on their right femurs. They were divided into two groups of 11 subjects each. In the control group, the animals received saline solution and in the study group, they received LMWH - enoxaparin - in a daily basis, during 28 days. After that period, the rats were submitted to euthanasia for femur assessment purposes. At the macroscopic study

  1. Filosofia da análise da estabilidade da liquidez

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    Rodrigo Antônio Chaves da Silva

    2005-07-01

    Full Text Available A informação foi considerada finalidade de nosso conhecimento, até o período em os pensadores e pesquisadores da contabilidade passaram a raciocinar sobre o conteúdo e o significado dos informes. Nesta busca da razão sobre os estados patrimoniais, surgiu a análise contábil que procura por meio de relações e identidades, o significado da dinâmica expressa da estaticamente na informação. O primeiro aspecto que surgiu no objeto de análise foi o estudo da liquidez, que é um dos principais exercícios do patrimônio. A estabilidade também é outro exercício básico e imprescindível, pois este é que promove o equilíbrio do organismo administrativo. A ciência contábil após a sua dignidade científica passou a trilhar caminhos esplendorosos, amparados em doutrina que permite alcançar os píncaros filosóficos. Os estudos concernentes aos aspectos de interação da estabilidade na liquidez são, complexos e somente com os recursos filosóficos da contabilidade se pode estudá-los com o panorama holístico e sublime. A filosofia da contabilidade não é alheia às suas práticas tecnológicas, podendo buscar pontos sublimes de panoramas abrangentes, para o estudo analítico da liquidez e estabilidade, observando todas as dimensionalidades e essencialidades de acontecimentos, na comprovação e orientação dos estados de ineficácia e eficácia patrimonial.

  2. Inibição da formação de abscesso abdominal em rato: mortalidade por sepse Inhibition of abdominal abscess formation in rat: Mortality by sepsis

    Directory of Open Access Journals (Sweden)

    Fernando Henrique Oliveira Carmo Rodrigues

    2005-03-01

    Full Text Available RACIONAL: Atribui-se aos abscessos intra-abdominais e às aderências peritoniais a função de isolar os processos sépticos e proteger o organismo da bacteremia. Por outro lado, esses fenômenos também dificultam o afluxo de fatores imunitários e antibióticos para a região infectada. OBJETIVO: Avaliar o efeito da prevenção de abscessos na sobrevida após peritonite bacteriana. MÉTODOS: Foram estudados 30 ratos Wistar machos que receberam solução de fezes a 50% intra-abdominal e que foram distribuídos em três grupos (n = 10. Grupo 1: controle (solução de fezes; grupo 2: solução de fezes mais solução salina a 0,9%; grupo 3: solução de fezes mais carboximetilcelulose a 1%, para inibir a formação de aderências. Os três grupos foram divididos em dois subgrupos (n = 5: subgrupo A: nova laparotomia, após 4 dias, para inspeção da cavidade abdominal; e subgrupo B: acompanhamento durante 30 dias para avaliação da mortalidade e da causa de morte. A análise estatística utilizou o teste exato de Fisher. RESULTADOS: O acréscimo de solução salina a 0,9% não aumentou a mortalidade do grupo. Entretanto, no grupo em que se acrescentou a solução de carboximetilcelulose, houve menor formação de abscessos, que também foram mais tênues e a mortalidade aumentou em relação ao grupo controle. CONCLUSÃO: A inibição na formação de aderências peritoniais e de abscessos acompanha-se de maior mortalidade decorrente do processo séptico intra-abdominal generalizado.

  3. Efeito da Gymnema sylvestre na elevação da lipidemia após administração de gordura de origem animal em ratos wistar - DOI: 10.4025/actascibiolsci.v26i3.1602 The influence of Gymnema sylvestre on the elevation of blood levels of lipids after administration of animal fat in male wistar rats - DOI: 10.4025/actascibiolsci.v26i3.1602

    Directory of Open Access Journals (Sweden)

    Márcia Regina Batista

    2004-04-01

    Full Text Available Neste trabalho investigamos a influência da Gymnema sylvestre (Gs na elevação da lipidemia após administração de gordura de origem animal em ratos machos Wistar (230g - 250g. Os animais, em jejum por 22 horas, foram divididos em 5 grupos que receberam via intragástrica às 8h os seguintes tratamentos: 1 2mL de gordura suína Batavo®/kg de peso corpóreo (GSB; 2 GSB + 500mg/kg de peso corpóreo de Gs (Gs 500mg; 3 GSB + 1g/kg de peso corpóreo de Gs (Gs 1g; 4 Gs 500mg; 5 Gs 1g. Os animais foram sacrificados 30min após a administração destas substâncias. Não observamos diferenças significativas na concentração sérica de lipídeos totais, triglicerídeos e colesterol total nos ratos que receberam GSB na ausência ou presença de Gs 500mg ou Gs 1g. Portanto, nossos resultados sugerem que a Gs não afeta a absorção intestinal de lipídeosThe aim of the present experiment was to investigate the influence of Gymnema sylvestre (Gs on the elevation of blood levels of lipids after administration of animal fat in male Wistar rats (230–250g. The animals, which were fasted for 22h, were separated in five groups that received (8:00 a.m intragastric administration of: 1 2ml of pork fat Batavo® /kg of body weight (PFB; 2 PFB+500mg/kg of Gs body weight (Gs 500mg; 3 PFB + 1g/kg of Gs body weight (Gs 1 g; 4 Gs 500mg; 5 Gs 1g. The animals were sacrificed 30min after the administration of these substances. No significant differences were verified in the serum concentration of total lipids, triglycerides and cholesterol in the animals, which received PBF in the presence or absence of Gs 500mg or Gs 1g. Therefore, the results showed that Gs could not affect the intestinal absorption of lipids

  4. Weight loss and morphometric study of intestinal mucosa in rats after massive intestinal resection: influence of a glutamine-enriched diet Perda de peso e estudo morfométrico da mucosa intestinal de ratos submetidos à ressecção subtotal de intestino delgado: influência do uso de dieta com glutamina

    Directory of Open Access Journals (Sweden)

    Sidney Resende Ribeiro

    2004-01-01

    standard-diet group. CONCLUSIONS: In rats with experimentally produced short-bowel syndrome, glutamine-enrichment of an isonitrogenous test diet was associated with an improved adaptation response by the intestinal mucosa but not reduced weight loss. However, the adaptation response in the group receiving the glutamine-enriched diet was not improved over that for the group fed regular chow.A síndrome do intestino curto é responsável por alterações metabólicas que comprometem o estado nutricional do paciente. A glutamina é nutriente essencial para os enterócitos. OBJETIVOS: Estudou-se o efeito da administração de dieta com glutamina, em ratos submetidos à ressecção subtotal do intestino delgado, avaliando a perda de peso pós-operatória e a morfometria da mucosa intestinal. MÉTODOS: Foram constituídos três grupos de ratos Wistar machos recebendo as seguintes dietas: com glutamina (grupo EG, sem glutamina (grupo EsG, e a dieta padrão do laboratório (grupo ER. A ressecção intestinal foi realizada em todos os animais incluindo-se a válvula íleo-cecal, com remanescente jejunal de apenas 25cm a partir do piloro, anastomosado látero-lateralmente ao cólon ascendente. A diferença entre o peso inicial real e ao final do experimento no 20º dia pós-operatório foi registrada antes do sacrifício. Fragmentos de mucosa do duodeno e jejuno foram colhidos e corados com hematoxilina-eosina. Realizou-se o estudo morfométrico da mucosa intestinal, por meio de sistema digital associado ao microscópio ótico. Mediu-se a altura das vilosidades, a profundidade das criptas e a extensão total da mucosa intestinal. RESULTADOS: A perda de peso entre os três grupos não diferiu estatisticamente. A análise da morfometria da mucosa intestinal mostrou diferença significativa: 1 animais do grupo Enterectomia com Glutamina (EG apresentaram vilosidade duodenal significativamente maior que o grupo EsG (p< 0,05, mas não diferiram do grupo que recebeu dieta padrão do

  5. Functional analysis of aldehyde oxidase using expressed chimeric enzyme between monkey and rat.

    Science.gov (United States)

    Itoh, Kunio; Asakawa, Tasuku; Hoshino, Kouichi; Adachi, Mayuko; Fukiya, Kensuke; Watanabe, Nobuaki; Tanaka, Yorihisa

    2009-01-01

    Aldehyde oxidase (AO) is a homodimer with a subunit molecular mass of approximately 150 kDa. Each subunit consists of about 20 kDa 2Fe-2S cluster domain storing reducing equivalents, about 40 kDa flavine adenine dinucleotide (FAD) domain and about 85 kDa molybdenum cofactor (MoCo) domain containing a substrate binding site. In order to clarify the properties of each domain, especially substrate binding domain, chimeric cDNAs were constructed by mutual exchange of 2Fe-2S/FAD and MoCo domains between monkey and rat. Chimeric monkey/rat AO was referred to one with monkey type 2Fe-2S/FAD domains and a rat type MoCo domain. Rat/monkey AO was vice versa. AO-catalyzed 2-oxidation activities of (S)-RS-8359 were measured using the expressed enzyme in Escherichia coli. Substrate inhibition was seen in rat AO and chimeric monkey/rat AO, but not in monkey AO and chimeric rat/monkey AO, suggesting that the phenomenon might be dependent on the natures of MoCo domain of rat. A biphasic Eadie-Hofstee profile was observed in monkey AO and chimeric rat/monkey AO, but not rat AO and chimeric monkey/rat AO, indicating that the biphasic profile might be related to the properties of MoCo domain of monkey. Two-fold greater V(max) values were observed in monkey AO than in chimeric rat/monkey AO, and in chimeric monkey/rat AO than in rat AO, suggesting that monkey has the more effective electron transfer system than rat. Thus, the use of chimeric enzymes revealed that 2Fe-2S/FAD and MoCo domains affect the velocity and the quantitative profiles of AO-catalyzed (S)-RS-8359 2-oxidation, respectively.

  6. Beneficial effects of hyperosmotic perfusion in the myocardium after ischemia/reperfusion injury in isolated rat hearts Efeitos benéficos da perfusão hiperosmótica no miocárdio após lesão isquemia/reperfusão em corações isolados de ratos

    Directory of Open Access Journals (Sweden)

    Yong Cao

    2013-03-01

    Full Text Available OBJECTIVE: A simple method to reduce the ischemia/reperfusion injury that can accompany cardiac surgery would have great clinical value. This study was to investigate the effect of hyperosmotic perfusion on ischemia/reperfusion injury in isolated perfused rat hearts. METHOD: Forty male Sprague-Dawley rats were randomly divided either to have their isolated hearts perfused with normal osmotic buffer or buffer made hyperosmotic by addition of glucose. Hearts were then subjected to 30 min ischemia followed by 30 min reperfusion. Coronary flow, time to ischemic arrest, reperfusion arrhythmia, and ventricular function were recorded. Creatine phosphokinase leakage into the coronary artery, and myocardial content and activity of superoxide dismutase and catalase were also examined. RESULTS: Rat hearts with hyperosmotic perfusion showed higher coronary flow, a prolonged time to ischemic arrest (10.60 vs. 5.63 min, POBJETIVO: Um método simples para reduzir a lesão de isquemia/reperfusão que pode acompanhar a cirurgia cardíaca teria grande valor clínico. O objetivo deste estudo foi investigar o efeito da perfusão hiperosmótica na isquemia/reperfusão em corações isolados de ratos perfundidos. MÉTODOS: Quarenta ratos machos Sprague-Dawley foram divididos aleatoriamente e tiveram os seus corações isolados perfundidos com tampão osmótico normal ou tampão hiperosmótico com a adição de glucose. Os corações foram então submetidos a 30 minutos de isquemia, seguida de 30 min de reperfusão. O fluxo coronariano, tempo de parada isquêmica, arritmia de reperfusão e da função ventricular foram registrados. Vazamento creatinofosfoquinase na artéria coronária, o miocárdio e atividade de superóxido dismutase e catalase foram também examinados. RESULTADOS: Crações de ratos com perfusão hiperosmótica apresentaram maior fluxo coronariano, tempo prolongado de parada isquêmica (10,60 vs. 5,63 min, P<0,005, menor pontuação de reperfus

  7. Efeitos da suplementação de creatina e do treinamento de potência sobre a performance e a massa corporal magra de ratos Effects of creatine supplementation and power training on performance and lean body mass of rats

    Directory of Open Access Journals (Sweden)

    Frederico S.C. Franco

    2007-10-01

    Full Text Available INTRODUÇÃO: A creatina é um dos suplementos mais usados por atletas para incrementar a síntese protéica e aumentar a massa e força muscular. OBJETIVO: Investigou-se os efeitos da suplementação de creatina associada a um programa de treinamento de potência (saltos verticais sobre a performance e a composição da massa corporal magra de ratos Wistar. MÉTODOS: Ratos Wistar adultos foram distribuídos em quatro grupos: SSC (sedentário sem creatina; SC (sedentário com creatina; ESC (exercício sem creatina e EC (exercício com creatina. Os animais receberam água e ração ad libitum. Os grupos SC e EC ingeriam dose de creatina diariamente, adotando o procedimento de carga (0,430g/kg p.c. por 7 dias e manutenção (0,070g/kg p.c. por 6 semanas. Os grupos EC e ESC foram submetidos a um regime progressivo de saltos verticais (5x10 saltos com 1 min de intervalo em tanque com água, 5 dias/semana, durante 7 semanas. A performance foi avaliada pelo tempo de execução das 5 séries de 10 saltos verticais e a composição da massa corporal magra (músculos e ossos foi avaliada pelas porções: água, proteína e gordura. RESULTADOS: A performance não foi afetada pela ingestão de creatina (p > 0,05. Os animais suplementados tiveram o percentual de proteína elevado e o de gordura reduzido (p 0,05. CONCLUSÃO: A suplementação de creatina não afetou a performance dos animais, mas alterou a massa corporal magra. A suplementação de creatina e o programa de treinamento de potência, de forma independente, elevaram o percentual de proteína dos músculos e ossos e reduziram o percentual de gordura, sem alterar o percentual de água.INTRODUCTION: Creatine is one of the supplements most used by athletes in order to increase protein synthesis and consequently muscle mass and strength. OBJECTIVE: This study investigated the effects of creatine intake on the performance and lean body mass of Wistar rats. METHODS: Male Wistar rats were allocated

  8. Catecholaminergic development of fetal rat ventral mesencephalon : Characterization by high-performance liquid chromatography with electrochemical detection and immunohistochemistry

    NARCIS (Netherlands)

    Tomasini, R; Kema, IP; Muskiet, FAJ; Meiborg, G; Staal, MJ; Go, KG

    We determined dopamine (DA), noradrenaline (NA), and adrenaline (A), as well as immunohistochemically stained tyrosine hydroxylase (TH) and DA in dissected rat ventral mesencephalon (VM) tissue from Embryonic Day (ED) 14 to Postnatal Day (P) 17. Whole VM tissue DA, NA, and A contents increased with

  9. Efeito da associação entre obesidade neuroendócrina e exócrina experimental sobre a pressão arterial de cauda e o metabolismo de glicose de ratos Wistar Effects of the association of experimental neuroendocrine and exocrine obesity on tail blood pressure and glucose metabolism in Wistar rats

    Directory of Open Access Journals (Sweden)

    Andréa Paula Pastore

    2010-06-01

    Full Text Available OBJETIVO: Estudar dois modelos de obesidade, exócrina e endócrina, e sua associação sobre a pressão arterial de cauda (PAC, o peso corporal (PC, o metabolismo glicídico (ISI e gordura epididimal relativa (GER. MÉTODOS: Foram estudados ratos machos da cepa Wistar. O grupo MSG recebeu glutamato monossódico no período neonatal. Aos 3 meses de idade parte desses animais passou a receber dieta cafeteria (CAF. Os animais receberam controle salina no período neonatal. Durante 12 semanas foram pesados (PC e tiveram a pressão arterial de cauda (PAC aferida. O Teste de Tolerância Oral à Glicose foi realizado e o Índice de Sensibilidade à Insulina (ISI, calculado. O peso ventricular relativo (PVR e a gordura epididimal relativa (GER também foram calculados. RESULTADOS: Não se verificou alterações no PC e na PAC. A obesidade induzida pela administração de MSG e CAF, isoladamente, promoveu aumento da resistência à insulina (WST = 23,25 ± 9,31; CAF = 15,92 ± 9,10*; MSG = 13,41 ± 3,84* mg-1mU-1, p OBJECTIVE: To study two different models of obesity, exocrine and endocrine, and its association on tail arterial pressure (TAP, body weight (BW, glucose metabolism and visceral fat content. METHODS: Male Wistar rats were studied. The MSG group was composed by rats that received of MSG in neonatal period. At the 3rd month of life, part of these animals received cafeteria diet. Animals received saline control in the neonatal period. In the 12 weeks of study, body weight and blood pressure were measured twice a week. In the end of this period on, Oral Glucose Tolerance Test (OGTT was performed and the Insulin Sensitivity Index (ISI was calculated, also the left Relative Ventricular Weight (RLW and Relative Epididimal Fat Weight (REFW were obtained. RESULTS: No changes on BW and TAP were verified. The obesity induced by MSG and CAF, individually, let to increases on insulin resistance (WST = 23,25 ± 9,31; CAF = 15,92 ± 9,10*; MSG = 13,41 ± 3

  10. Comparison of the indirect fluorescent antibody test and modified agglutination test for detection of anti-Toxoplasma gondii antibodies in rats / Comparação da reação de imunofluorescência indireta e do teste de aglutinação modificado na detecção de anticorpos anti-Toxoplasma gondii em ratos

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    Roberta Lemos Freire

    2010-09-01

    Full Text Available The toxoplasmosis is a zoonosis caused by Toxoplasma gondii and affects a lot of species of carnivores and omnivores, including the human. The rodents are important in the transmition cycle because they act as an infection font to felines, the definitive host of this protozoan. The objective of this work was to evaluate the Modified Agglutination Test (MAT for the serologic diagnosis of toxoplasmosis in rats, comparing with the Indirect Fluorescent Antibody Test (IFAT, which has been considered the golden standard in animal toxoplasmosis diagnosis. Kappa test was used for comparing the serologic tests (IFAT and MAT and for determination of cutoff appropriate to MAT in this animal species. 182 rats were caught on local recycling of solid waste and solid residue storage in Londrina city, Paraná. Out of the 182 rats, nine (4.94% were positive to IFAT at a dilution of 1:16, and 17 (9.34% and five (2.75% were reactive to MAT in dilutions 1:25 and 1:50, respectively. The comparison of results between the techniques presented kappa coefficients of 0.26 and 0.55, respectively at 1:25 and 1:50 dilutions of MAT. It can be concluded that the dilution 1:50 is the most suitable to be used as cutoff for detecting T. gondii antibodies in rats using MAT, because agreed with IFAT.A toxoplasmose é uma zoonose causada pelo Toxoplasma gondii que acomete várias espécies carnívoras e onívoras, incluindo o ser humano. Os roedores são importantes na cadeia epidemiológica da doença por servirem de fonte de infecção aos felídeos, os hospedeiros definitivos deste protozoário. O objetivo deste trabalho foi avaliar o Teste de Aglutinação Modificada (MAT na detecção de anticorpos contra T. gondii em ratos, comparando-o à Reação de Imunofluorescência Indireta (RIFI, considerada padrão ouro para o diagnóstico da toxoplasmose animal. Empregou-se o teste kappa para a comparação dos testes sorológicos (RIFI e MAT e para a determinação do ponto de corte

  11. Evaluation of radioprotective effect of carnosine (beta- alanyl-1- histidine on the wound healing in rats Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina no processo de cicatrização em ratos

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    Rosana Aramaki Tanaka

    2005-09-01

    Full Text Available The purpose of this study was to evaluate the radioprotective effect of carnosine (beta- alanyl-1-histidine on the wound healing in rats. Therefore, 48 male rats were submitted to a surgical procedure to perform a rectangular wound in the anterior-dorsal region. The animals were divided into 4 experimental groups randomly chosen: control; irradiated; carnosine irradiated and carnosine group. The irradiated and carnosine irradiated group were exposed to a dose (6Gy of gamma irradiation, in the whole body, 72 hours after surgery. The carnosine and carnosine irradiated groups, in addition to the surgical procedure and the irradiation, received two doses of carnosine aqueous solution, the first one being injected 48 hours after surgery, and the second one 1 hour and 30 minutes before irradiation. The tissue repair of the 4 groups was evaluated at 4, 7, 14, and 21 days after inflicting the wound, by morphological, histochemical and histophysical methods. At all examined periods, it could be observed that the animals from the carnosine irradiated group presented a better developed granulation tissue than the irradiated group and closely similar to that of the control group. Thus, under the experimental conditions used, it was possible to conclude that carnosine is an effective radioprotective substance.O objetivo deste estudo foi avaliar o efeito radioprotetor da carnosina (beta-alanil-1-histidina no processo de cicatrização em ratos. Para isto, 48 ratos machos foram submetidos a um procedimento cirúrgico para realização de uma ferida retangular na região dorsal anterior. Os animais foram divididos aleatoriamente em 4 grupos experimentais: controle, irradiado, carnosina irradiado e carnosina. Os grupos carnosina e carnosina irradiado foram exposto a uma dose de corpo todo de 6 Gy de radiação gama, 72 horas após a cirurgia para confecção da ferida. O grupo carnosina e carnosina irradiado, adicionalmente, ao procedimento cirúrgico e a

  12. Analysis of hyaluronic acid concentration in rat vocal folds during estral and gravidic puerperal cycles Análise da concentração do ácido hialurônico nas pregas vocais de ratas durante o ciclo estral e ciclo gravídico-puerperal

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    José Eduardo de Sá Pedroso

    2009-10-01

    Full Text Available Hormone plays an important role in the larynx. Among other substances, vocal folds contain hyaluronic acid, which tissue concentration may vary according to hormone action. AIM: the objective of this study is to analyze hyaluronic acid concentration in the vocal folds during estral and gravidic-puerperal cycles. MATERIALS AND METHODS: Experimental study. 40 adult rats were divided into two groups. In the first group we used 20 rats to establish the concentration of hyaluronic acid during the estral cycle and in the second group, 20 animals were submitted to the same procedure but during the gravidic-puerperal cycle. RESULTS: Variations in hyaluronic acid concentration was not observed during the estral cycle. In the gravidic puerperal cycle group, an increase in hyaluronic acid concentration was observed in the puerperal subgroup. Comparing the two groups of estral and gravidic-puerperal cycles, no difference was observed. CONCLUSIONS: In comparing all subgroups of estral and gravidic-puerperal cycles, an increase in hyaluronic acid concentration was noticed only in the puerperal phase.Os hormônios exercem importante influência sobre a laringe. A prega vocal contém, entre outras substâncias, o ácido hialurônico, cuja concentração nos tecidos pode variar com a ação dos hormônios. OBJETIVO: O objetivo deste trabalho é analisar comparativamente a concentração do ácido hialurônico nas pregas vocais de ratas durante o ciclo estral e ciclo gravídico-puerperal. FORMA DE ESTUDO: Experimental. MATERIAL E MÉTODO: Foram utilizadas 40 ratas adultas, divididas em dois grupos, no primeiro grupo utilizamos 20 ratas para determinação da concentração do ácido hialurônico no ciclo estral, no segundo grupo, também de 20 animais, foi realizado o mesmo experimento no ciclo gravídico-puerperal. RESULTADOS: No grupo do ciclo estral não observou-se variação da concentração do ácido hialurônico. No grupo do ciclo grav

  13. Cicatrização da membrana timpânica na timpanocentese com laser de argônio comparado à técnica com microlanceta: estudo experimental em ratos Tympanic membrane healing in myringotomies performed with argon laser or microknife: an experimental study in rats

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    Lucio Almeida Castagno

    2006-12-01

    Full Text Available Otite média secretora (OMS e otite média aguda recorrente (OMAR podem necessitar tratamento cirúrgico para adequada ventilação da orelha média. A abertura clássica do tímpano (timpanocentese requer incisão por microlanceta sob controle de microscópio cirúrgico e mantém-se patente por alguns dias. Estudos recentes sugerem que a timpanocentese feita por diferentes lasers pode permanecer permeável por maior tempo, o que possibilitaria a normalização da otite média. MATERIAL E MÉTODOS: Neste estudo experimental 34 ratos linhagem Wistar, albinos, machos adultos pesando cerca de 300g, foram anestesiados com cetamina 27 mg/kg e xilazina 2,7 mg/kg. A seguir, foram submetidos à timpanocentese incisional com microlanceta no ouvido direito (ML-OD, e à timpanocentese mediada por laser de argônio no ouvido esquerdo (LA-OE. RESULTADOS: Não houve diferença significativa no tempo de cicatrização das timpanocenteses feitas com laser de argônio ou microlanceta. Todas as timpanocenteses cicatrizaram em 10 dias. CONCLUSÃO: A timpanocentese com laser de argônio apresentou patência e cicatrização semelhantes à técnica clássica com microlanceta realizada em ratos Wistar sem enfermidades de orelha média.Secretory otitis media (SOM and recurrent acute otitis media (RAOM may require surgical treatment to proper ventilate the middle ear. Incisional myringotomy is usually done under microscopy with a micro-knife , but it remains patent for just a few days. Recent research indicates that laser assisted myringotomies remain open much longer, allowing middle ear ventilation and healing. MATERIAL AND METHODS: In this experimental study 34 white, male, adult, Wistar rats, without middle ear disease were submited to anesthesia with ketamine 27 mg/kg and xylazine 2,7 mg/kg. Incisional myringotomy was done on the right ear, while laser myringotomy was done on the left. Myringotomies were evaluated periodically until healing. RESULTS: The healing

  14. Avaliação do efeito fisiológico da farinha de semente de abóbora (Cucurbita maxima, L. no trato intestinal de ratos Evaluation of the pumpkin (Cucurbita maxima, L. seed flour effects on the intestinal tract of rats

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    Matilde Pumar

    2008-12-01

    Full Text Available O presente trabalho teve por objetivo avaliar o efeito fisiológico da Farinha de Semente de Abóbora (FSA no trato intestinal de ratos Wistar machos recém desmamados, submetidos a dietas experimentais contendo Farinhas de Semente de Abóbora (FSAs integral, peneirada e residual, por 10 dias. As dietas experimentais foram obtidas substituindo-se 30% do valor total de amido e dextrina, da ração controle, pela FSA correspondente. As farinhas e rações controle e experimentais foram caracterizadas quimicamente. Os animais divididos em grupos foram avaliados quanto ao crescimento, consumo, material fecal e pH cecal. As FSAs e rações experimentais apresentaram relevantes teores de fibras alimentares, proteínas e lipídios. Dietas contendo FSAs integral, peneirada e residual levaram a um ganho ponderal e ingestão similar entre todos os grupos. Em relação ao grupo controle, os animais experimentais submetidos a dietas contendo FSAs apresentaram maior volume e peso fecais (p This study has the aim to evaluate the physiological effects of the Pumpkin Seed Flour (PSF on the intestinal tract of newly weaned male Wistar rats fed on diets containing whole Sifted and Residual - PSF for ten days. The experimental diets were prepared substituting 30% of the total content of starch and dextrin of the control diet for the corresponding PSF. The control and the experimental diets were chemically characterized. The animals were divided into four groups in order to evaluate growth, ingestion, feces, and cecal pH. The PSFs and the experimental diets presented high contents of alimentary fibers, proteins, and lipids. Similar ingestions of diets containing whole Sifted and Residual - PSF resulted in body weight gain. Rats on diets containing PSFs showed higher feces volume and weight (p > 0.05 in addition to an expressive insoluble fiber excretion, especially in the Residual - PSF group. Only the Residual PSF presented a significant reduction (p < 0.05 in the

  15. Electrophysiologic assessment of regeneration in rat sciatic nerve repair using suture, fibrin glue or a combination of both techniques Avaliação eletrofisiológica da eficácia de três tipos de reparo após a secção do nervo ciático do rato

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    Roberto Sergio Martins

    2005-09-01

    Full Text Available We evaluated the repair of seccioned rat sciatic nerve by the comparison of electrophysiologic parameters. The repair was effected with suture (group A, fibrin glue (group B or a combination of both techniques (group C. The amplitude, latency and conduction velocity of the motor and nerve action potentials were assessed before the nerve section and at reoperation after 24 weeks. There was no difference between the groups when the nerve action potential was evaluated. Rats of group B presented better results than those of group A (pForam comparados os parâmetros obtidos na avaliação eletrofisiológica do potencial de ação do nervo e do potencial de ação motor antes e após 24 semanas do reparo no nervo ciático do rato previamente seccionado no lado direito com a utilização de sutura (grupo A, adesivo de fibrina (grupo B ou uma combinação das duas técnicas (grupo C. Não houve diferença entre os grupos na avaliação do potencial de ação do nervo. Quando consideradas a latência e a velocidade de condução mensurados na reoperação e a razão entre a velocidade de condução medida na reoperação e o mesmo parâmetro antes da secção do nervo, durante a mensuração do potencial de ação motor, os animais do grupo B apresentaram melhores resultados em relação aos do grupo A (p<0,05. Os animais do grupo C apresentaram melhores resultados em comparação com os do grupo A quando considerada a razão entre a velocidade de condução medida 24 semanas do reparo e antes da secção do nervo durante a avaliação do potencial de ação motor. Conclui-se que os animais em que o reparo dos nervos foi realizado com o adesivo de fibrina apresentaram melhores resultados em comparação com a sutura quando considerados os parâmetros obtidos na mensuração do potencial de ação motor.

  16. Efeitos do exercício crônico sobre a concentração circulante da leptina e grelina em ratos com obesidade induzida por dieta Effects of the chronic exercise on the circulating concentration of leptin and ghrelin in rats With diet-induced obesity

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    Ricardo Eguchi

    2008-06-01

    Full Text Available A obesidade vem se tornando uma das maiores epidemias mundiais, dessa forma, conhecer sua etiologia e mecanismos que regulam seu desenvolvimento é de grande relevância para o seu Tratamento. Portanto, o objetivo do presente estudo foi avaliar os efeitos da obesidade exógena induzida pela dieta de cafeteria e da atividade física crônica em ratos, sobre a adiposidade e a concentração sérica dos hormônios reguladores do balanço energético (leptina e grelina. Foram utilizados 32 ratos Wistar machos, divididos em quatro grupos: Sedentário alimentado com dieta padrão (SN, sedentário alimentado com dieta de cafeteria (SC, treinado alimentado com dieta padrão (TN e treinado alimentado com dieta de cafeteria (TC. A dieta de cafeteria aumentou significativamente a adiposidade central (RET e visceral (EPI (pObesity is becoming one of the biggest worldwide epidemics. Therefore, knowing its etiology and mechanisms that regulate its development is of great relevance for its treatment. Thus, the aim of the present study was to evaluate the effects of obesity induced by the palatable hyperlipidic diet and of the chronic physical activity in rats, on the adiposity and the serum concentration of regulating hormones of the energy balance (leptin and ghrelin. 32 male Wistar rats were divided in four groups: Sedentary fed with chow diet (SN, sedentary fed with cafeteria diet (SC, trained fed with chow diet (TN and trained fed with cafeteria diet (TC. The cafeteria diet led to a significant increase of central (RET and visceral (EPI adiposity (p<0.05. Conversely,the exercise training minimized the effect of the cafeteria diet, diminishing the central and visceral adiposity. Leptin was also increased in the groups fed with the cafeteria diet, suggesting increase of the resistance to the action of this hormone. Chronic physical activity did not hinder the development of hyperleptinemia. Reduction in the serum ghrelin concentration was observed only in

  17. Influence of estrogen deficiency and tibolone therapy on trabecular and cortical bone evaluated by computed radiography system in rats Influência da deficiência estrogênica e do tratamento com tibolona no osso trabecular e cortical avaliada pelo sistema de radiografia computadorizada em ratas

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    Ana Carolina Bergmann de Carvalho

    2012-03-01

    Full Text Available PURPOSE: To verify the effects of tibolone administration on trabecular and cortical bone of ovariectomized female rats by computed radiography system (CRS. METHODS: The experiment was performed on two groups of rats previously ovariectomized, one received tibolone (OVX+T while the other did not (OVX, those groups were compared to a control group (C not ovariectomized. Tibolone administration (1mg/day began thirty days after the ovariectomy and the treatment remained for five months. At last, the animals were euthanized and femurs and tibias collected. Computed radiographies of the bones were obtained and the digital images were used to determine the bone optical density and cortical thickness on every group. All results were statistically evaluated with significance set at POBJETIVO: Verificar o efeito da administração de tibolona no tecido ósseo cortical e trabecular de ratas castradas através de radiografia computadorizada. MÉTODOS: O experimento foi realizado em dois grupos de ratas previamente ooforectomizadas, onde um grupo recebeu tibolona (OVX+T e o outro não (OVX. Esses grupos foram comparados a um grupo controle (C não ooforectomizado. A administração de tibolona (1mg/dia começou trinta dias após a ooforectomia e o tratamento teve duração de cinco meses. No final, os animais foram mortos e fêmures e tibias coletados. As radiografias computadorizadas dos ossos foram obtidas e as imagens digitais usadas para determinar a densidade óssea e a espessura cortical em todos os grupos. Todos os resultados foram avaliados estatisticamente com significância estabelecida a 5%. RESULTADOS: A administração de tibolona mostrou ser benéfica apenas para análise densitométrica da cabeça do fêmur, apresentando maiores valores de densidade comparada ao grupo OVX. Nenhuma diferença significativa foi encontrada para espessura óssea cortical. CONCLUSÃO: A ooforectomia ocasionou perda óssea nas regiões analisadas e a tibolona

  18. Influência do ciclo estral sobre a sensibilidade da resposta cronotrópica à norepinefrina em ratas submetidas a estresse agudo Influence of estrous cycle on the sensitivity of cronotropic answer to norepinephrine in rats submitted to acute stress

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    Ana Paula Tanno

    2002-03-01

    Full Text Available O estresse pode alterar a sensibilidade da resposta cronotrópica às catecolaminas em vários tecidos. O objetivo deste estudo foi avaliar a sensibilidade à norepinefrina (NE em átrios direitos de ratas submetidas ao estresse agudo por natação nas fases de estro e proestro. Ratas Wistar em estro ou proestro foram submetidas a uma sessão de 50 min de natação, após a qual foram anestesiadas e sacrifícadas. Os átrios direitos destes animais e de ratas controle foram isolados para obtenção de curvas concentração-efeito à NE antes e após o bloqueio dos sistemas de metabolização das catecolaminas (fenoxibenzamina + estradiol. Os dados foram analisados por ANOVA ou teste t de Student. Não houve diferenças de sensibilidade à NE entre as fases de estro e proestro nos tecidos isolados de animais controle (p>0,05. No proestro, a natação induziu supersensibilidade à NE (pStress may change the response to catecholamines in many tissues. The aim of this study was to investigate the influence of the estrous cycle on the sensitivity to norepinephrine in right atria from female rats submitted to a single swimming session. Wistar female rats were submitted to one swimming session at estrus or proestrus. Immediately after the stress session, the animal was sacrificed and its right atria set up for isometric recording of spontaneous beating. Concentration-effect curves to norepinephrine were obtained before and after inhibition of uptake1 (phenoxibenzamine and uptake2 (estradiol. Swimming stress did not change the sensitivity to noradrenaline in right atria from rats at estrus. However, at proestrus swimming induced supersensitivity to norepinephrine (pD2 control: 7.14 ± 0.03 vs. pD2 swimming: 7.55 ± 0.04; p< 0.05. Moreover at proestrus, the inhibition of the uptake systems induced a lower shift to the left in the concentration-effect curves to norepinephrine compared to the estrus. Changes on the uptake systems seem to be involved in the

  19. Estudo da regeneração de nervos tibiais de ratos Wistar em sutura primária com "gap" e sem "gap", cobertos por segmentos de veia Study of tibial nerve regenration in Wistar rats in primary neurorrhaphy with and without gap, wrapped in vein segments

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    Ewerton Bastos dos Santos

    2012-01-01

    Full Text Available OBJETIVO: O presente estudo comparou, em ratos da raça Wistar, a regeneração nervosa nas suturas epineurais com espaçamento de 1,0mm (com "gap" e sem espaçamento (sem "gap", ambos cobertos com tubo de veia jugular externa, através da contagem de motoneurônios no nível da medula espinhal entre L3 e S1, marcados por meio de exposição do nervo tibial ao Fluoro - Goldâ (FG. MÉTODO: Os nervos tibias de ambos os lados foram seccionados e foram realizadas suturas epineurais com "gap" e, no lado contralateral, sem "gap" sendo que as suturas foram cobertas com tubo de veia. Após quatro meses do procedimento cirúrgico, os nervos tibias foram expostos ao FG, perfundidos e realizada a contagem dos motoneurônios na medula espinhal. RESULTADOS: Para a análise estatística foi utilizado o teste de Wilcoxon pareado, onde obtivemos um resultado estatisticamente significante entre o número de motoneurônios do grupo com "gap" em relação ao sem "gap" (p= 0,013. CONCLUSÃO: Obtivemos melhores resultados na contagem de motoneurônios daqueles nervos onde haviam sido realizadas as suturas primárias sem "gap", quando comparados com as suturas com "gap". Nível de Evidência: Estudo Experimental.OBJECTIVE: This study compared nerve regeneration in Wistar rats, using epineural neurorrhaphy with a gap of 1.0 mm and without a gap, both wrapped with jugular vein tubes. Motor neurons in the spinal cord between L3 and S1 were used for the count, marked by exposure of the tibial nerve to Fluoro-Gold (FG. METHOD: The tibial nerves on both sides were cut and sutured, with a gap on one side and no gap in the other. The sutures were wrapped with a jugular vein. Four months after surgery the tibial nerves were exposed to Fluoro-Gold and the motor neuron count performed in the spinal cord. RESULTS: The results were statistically analyzed by the paired Wilcoxon test. There was a statistical difference between the groups with and without gap in relation to the

  20. DA-6034 Induces [Ca(2+)]i Increase in Epithelial Cells.

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    Yang, Yu-Mi; Park, Soonhong; Ji, Hyewon; Kim, Tae-Im; Kim, Eung Kweon; Kang, Kyung Koo; Shin, Dong Min

    2014-04-01

    DA-6034, a eupatilin derivative of flavonoid, has shown potent effects on the protection of gastric mucosa and induced the increases in fluid and glycoprotein secretion in human and rat corneal and conjunctival cells, suggesting that it might be considered as a drug for the treatment of dry eye. However, whether DA-6034 induces Ca(2+) signaling and its underlying mechanism in epithelial cells are not known. In the present study, we investigated the mechanism for actions of DA-6034 in Ca(2+) signaling pathways of the epithelial cells (conjunctival and corneal cells) from human donor eyes and mouse salivary gland epithelial cells. DA-6034 activated Ca(2+)-activated Cl(-) channels (CaCCs) and increased intracellular calcium concentrations ([Ca(2+)]i) in primary cultured human conjunctival cells. DA-6034 also increased [Ca(2+)]i in mouse salivary gland cells and human corneal epithelial cells. [Ca(2+)]i increase of DA-6034 was dependent on the Ca(2+) entry from extracellular and Ca(2+) release from internal Ca(2+) stores. Interestingly, these effects of DA-6034 were related to ryanodine receptors (RyRs) but not phospholipase C/inositol 1,4,5-triphosphate (IP3) pathway and lysosomal Ca(2+) stores. These results suggest that DA-6034 induces Ca(2+) signaling via extracellular Ca(2+) entry and RyRs-sensitive Ca(2+) release from internal Ca(2+) stores in epithelial cells.

  1. A Sintaxe da Janela

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    Luís Antônio Jorge

    1993-12-01

    Full Text Available A preocupação fundamenta! do trabalho é de caráter ontológico: esboçar o problema das significações apriorísticas e autônomas dos elementos que compõem a sintaxe arquitetônica. O trabalho elege um desses elementos: a janela. Este artigo parte da aproximação histórica entre a janela e a secção da pirâmide visual, construção imaginária da teoria da perspectiva renascentista. A consideração da janela na linguagem arquitetônica implica reconhecer uma ambigüidade essencial: a janela deve inserir-se ordenadamente no plano da parede - raciocínio típico da modenatura - mas também relacionar-se com a imagem vista através dela, que extrapola o plano, ressaltando a tridimensionalidade - herança da perspectiva renascentista, fundamentada no espaço euclidiano. Ver janela ou ver através da janela são alternativas que se colocam tanto ao observador interno ao edifício, quanto ao externo. Texto de duplaface, a sintaxe da janela versa sobre o relacionamento desses dois lados

  2. Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats Avaliação por morfometria computadorizada das alterações histopatológicas da parede cólica em segmentos com e sem trânsito intestinal em ratos

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    Marcos Vieira de Sousa

    2008-10-01

    Full Text Available PURPOSE: To evaluate histopathological alterations of the colon wall in segments with and without intestinal transit, by computer-assisted imaging, and to correlate these with the length of time diversion. METHODS: Thirty male Wistar rats were subjected to intestinal transit diversion by a proximal colostomy and distal mucosa fistula. The animals were divided into three experimental groups according to how long after the initial surgical procedure they were sacrificed: six, twelve and eighteen weeks. Colon segments with and without transit were subjected to histopathological study. The variables colon crypt length, mucosal ulceration, muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria, vascular congestion, number of caliciform cells, inflammatory grade and degree of inflammation, comparing the two colon segments in the different experimental groups were studied. Intestinal crypt length, muscle layer thickness of the mucosa, submucosa and muscularis propria and caliciform cells were measured by computer-assisted imaging method. Mean equality, variance analysis and correlation tests were used in the statistical analysis, and the significance level was set at 5%. RESULTS: Comparison between segments with and without transit showed that the latter presented reduced length of colon crypts and increased muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria. There were greater quantities of ulceration of the mucosal and greater degree of inflammation with increasing time without transit. Mucosal ulceration, submucosal vascular congestion, increased thickness of the submucosal and muscularis propria layers, presence of caliciform cells, inflammatory infiltrate and inflammatory grade correlated significantly with the length of time without transit. CONCLUSIONS: Histological alterations occurred in all layers of the colon wall, in the segments without intestinal transit. Ulcerations in the

  3. Estudo comparativo da eficácia do etanol e do ácido L-glutâmico na prevenção da calcificação das cúspides e parede aórtica porcina: estudo experimental em ratos Comparative study on the efficacy of ethanol and of l-glutamic acid for preventing calcification of pig cusps and aortic wall: experimental study in rats

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    George Ronald Soncini da ROSA

    2002-06-01

    álcio/ mg tecido, E80% 30 dias (9,47 ± 2,59mg cálcio/mg tecido e E80% 60 dias (23,56±7,75 mg cálcio/mg tecido no grupo de AG 0,8% 15 dias (4,31±0,85 mg cálcio/mg tecido, AG 0,8% 30 dias (7,69±1,48 mg cálcio/mg tecido e AG 0,8% 60 dias (20,50± 1,22 mg cálcio/mg tecido com o grupo controle GDA 15 dias (7,34±1,32 mg cálcio/mg tecido, GDA 30 dias (9,28±0,76 mg cálcio/mg tecido e GDA 60 dias (27,60±1,08 mg cálcio/mg tecido. Na avaliação microscópica da cúspide aórtica houve uma progressiva calcificação naquelas submetidas à fixação com GDA. Este processo foi parcialmente encontrado com o AG 0,8% e totalmente ausente com o E80%. Quanto à avaliação referente aos segmentos da parede aórtica, também evidenciou-se progressiva calcificação, não sendo inibida pelos tratamentos com AG 0,8% e E80%. CONCLUSÕES: O pré-tratamento com etanol a 80% inibiu a calcificação nas cúspides aórticas porcinas, entretanto, não teve a mesma eficácia na parede aórtica. Contudo, o ácido L-glutâmico a 0,8% demonstrou minimizar a calcificação na parede aórtica. Estudos devem ser feitos para evidenciar se a ação anticalcificante do etanol a 80% mantém-se nas biopróteses aórticas porcinas se estas forem implantadas no sistema circulatório.INTRODUCTION: The glutataldehyde (GDA treated pigs cusps are one of most employed tissues in bioprosthesis, but is late post-implant calcification is main cause of its failure. BACKGROUND: This study aims at comparing and analyzing two methods (ethanol 80% and l-glutamic acid 0.8% to prevent calcification in pig cusps and aortic wall implanted subcutaneously in rats, the cusps and aortic wall segments of the control were in glutaraldehyde (GDA, during a 15, 30 and 60 days period after the implant. MATERIAL AND METHODS: We used 45 young rats, distributed in 3 groups of 15 rats each, which in turn were subdivided in 3 subgroups of 5 rats each, in which we implanted one cusp and one aortic wall segment in 2 subcutaneous

  4. Efeitos da suplementação de creatina na captação de glicose em ratos submetidos ao exercício físico Effects of creatine supplementation on glucose uptake in rats submitted to exercise training

    Directory of Open Access Journals (Sweden)

    Thiago Onofre Freire

    2008-10-01

    Full Text Available Estudos recentes têm sugerido que a suplementação de creatina é capaz de modular a homeostase da glicose, aumentando sua captação pelos tecidos periféricos. O objetivo deste trabalho foi investigar o efeito da suplementação de creatina na tolerância à glicose e no conteúdo de glicogênio muscular e hepático em ratos submetidos ou não à atividade física por quatro e oito semanas. Ratos Wistar foram divididos em dois grupos: quatro e oito semanas de intervenção. Posteriormente, cada grupo foi subdividido em quatro subgrupos, de acordo com a ingestão do suplemento e o treinamento: controle cedentário, controle treinado, suplementado sedentário e suplementado treinado. Os animais tiveram livre acesso à água e ração; o grupo suplementado teve 2% de sua ração sob a forma de creatina monoidratada. Os grupos exercitados nadaram 40 minutos por dia, quatro dias por semana, com carga entre 2 e 5% do seu peso amarrado ao peito. Após quatro e oito semanas, o teste oral de tolerância à glicose e as dosagens de glicogênio muscular e hepático foram realizadas. Não foram observadas diferenças significativas entre os grupos no teste de tolerância oral à glicose e no conteúdo de glicogênio muscular e hepático. Este estudo mostrou que a suplementação de creatina não exerceu influência na tolerância à glicose nem nas concentrações de glicogênio em ratos submetidos ou não à atividade física por quatro ou oito semanas.Recently, studies have suggested that creatine supplementation can modulate glucose homeostasis by increasing glucose uptake in peripheral tissues. The aim of this study was to investigate the effects of creatine supplementation on glucose tolerance, muscle and hepatic glycogen content in rats submitted or not to physical activity for four and eight weeks. Wistar rats were divided in two groups: four and eight weeks of intervention. Afterwards, each group was subdivided in four subgroups, according to

  5. Efeito imunossupressor da ciclosporina intra-muscular administrada em diferentes períodos pós-operatórios em um modelo de transplante penetrante de córnea em ratos Immunosuppressive effect of intramuscular cyclosporine used at different post-operative period on a penetrating keratoplasty model in the rat

    Directory of Open Access Journals (Sweden)

    Roberto von Hertwig

    1999-12-01

    Full Text Available Objetivo: Avaliar o efeito imunossupressor da ciclosporina intramuscular (I.M., administrada por tempo limitado em diferentes períodos do pós-operatório, no transplante penetrante de córnea em um modelo experimental em rato, por meio de avaliação clínica e anátomo-patológica do enxerto corneano. Método: Foram utilizados ratos isogênicos Fischer como doadores e Lewis como receptores, em um modelo ortotópico de transplante de córnea. A administração de ciclosporina I.M. 10 mg/kg/dia foi iniciada em diferentes períodos nos grupos estudados: no pós-operatório imediato, no 7º dia pós-operatório e no 9º dia pós-operatório. A ciclosporina quando iniciada foi administrada até o 30º dia pós-operatório. Um grupo controle não recebeu a ciclosporina I.M. Os enxertos corneanos foram avaliadas clínica e histologicamente. Resultados: Rejeição foi observada nas primeiras três semanas do pós-operatório em 100% dos casos no grupo controle (n = 5 que não recebeu a ciclosporina. Os ratos tratados com ciclosporina (n = 15 apresentaram rejeição em apenas um caso, que teve curta evolução e poucos sinais clínicos. Os estudos histológicos confirmaram as avaliações clínicas. O grupo controle apresentou infiltrado no enxerto corneal com predomínio de linfócitos sobre neutrófilos com mais neovasos, com mais fibrose e com infiltrado inflamatório mais intenso do que os grupos tratados com ciclosporina. Conclusão: Os dados obtidos indicam que a ciclosporina I.M., pode ter efeito benéfico sobre o controle da rejeição do transplante de córnea, mesmo na sua fase ativa.Purpose: Corneal graft rejection suppression after intramus-cular cyclosporine administration at different periods of time was studied on a penetrating keratoplasty rejection model in the rat. Methods: Inbred Lewis rats were used as recipients and Fischer rats were used as donors in a orthotopic rejection model of corneal transplantation. Intramuscular injection

  6. Soft-food diet induces oxidative stress in the rat brain.

    Science.gov (United States)

    Yoshino, Fumihiko; Yoshida, Ayaka; Hori, Norio; Ono, Yumie; Kimoto, Katsuhiko; Onozuka, Minoru; Lee, Masaichi Chang-il

    2012-02-02

    Decreased dopamine (DA) release in the hippocampus may be caused by dysfunctional mastication, although the mechanisms involved remain unclear. The present study examined the effects of soft- and hard-food diets on oxidative stress in the brain, and the relationship between these effects and hippocampal DA levels. The present study showed that DA release in the hippocampus was decreased in rats fed a soft-food diet. Electron spin resonance studies using the nitroxyl spin probe 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl directly demonstrated a high level of oxidative stress in the rat brain due to soft-food diet feeding. In addition, we confirmed that DA directly react with reactive oxygen species such as hydroxyl radical and superoxide. These observations suggest that soft-food diet feeding enhances oxidative stress, which leads to oxidation and a decrease in the release of DA in the hippocampus of rats. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  7. Proteção da recuperação funcional do miocárdio pelo omeprazol após isquemia-reperfusão em corações isolados de ratos Myocardium functional recovery protection by omeprazole after ischemia-reperfusion in isolated rat hearts

    Directory of Open Access Journals (Sweden)

    Otoni Moreira Gomes

    2010-09-01

    Full Text Available OBJETIVO: Analisar efeitos do omeprazol na proteção da recuperação funcional de corações isolados de ratos submetidos à lesão de isquemia-reperfusão. MÉTODOS: Foram estudados 12 ratos Wistar, peso corpóreo médio de 280g. Após anestesia com injeção intra-abdominal de 10mg de cetamina e 2mg de xilazina, os corações foram removidos e mantidos em perfusão com solução Krebs-Henseleit (95%O2 e 5% CO2, 37ºC, 110-120mmHg de pressão de perfusão e pressão diastólica de 8 mmHg em sistema Langendorff, modificado, descartável, modelo FCSFA-ServCor (Comex Ltda.. Os seis corações do Grupo I (GI e os seis do Grupo II (GII foram submetidos a 20 minutos de isquemia e 30 minutos de reperfusão. Nos corações do Grupo II, imediatamente antes da isquemia, foram administrados via perfusão coronária 200mcg de omeprazol. Foram controlados frequência cardíaca (FC, fluxo coronário (FCo, pressão sistólica (PS, +dP/dt e -dP/dt, após estabilização (t0 e no final da reperfusão (t30. Empregou-se método não paramétrico de Kruskal-Wallis (P0,05 entre os valores de FCo e FC nos dois grupos. No final do período de reperfusão (t30, foram significantes (POBJECTIVE: To evaluate the myocardium contractility alterations of isolated hearts of rats, submitted to ischemia and reperfusion with and without administration of the omeprazole. METHODS: Twelve Wistar breed rats with 270g mean body weight was studied. After anesthesia by intraperitoneal injection of ketamine 10mg and xylazine 2mg, their hearts were removed and perfused with Krebs-Henseleit solution (95% of O2 and 5% of CO2, 37ºC, 110-120 mmHg perfusion pressure, 8 mmHg ventricular diastolic pressure in the São Francisco de Assis disposable Langendorff system model Comex Ltda, MG. The six hearts of Group I (GI and of the Group II (GII were submitted to 20 min ischemia and 30 min reperfusion. In GII hearts, intracoronary injection of omeprazole 200 mcg was done immediately before the

  8. Effect of the oral administration of monosodium glutamate during pregnancy and breast-feeding in the offspring of pregnant Wistar rats Efeito da administração de glutamato monossódico durante a gestação e amamentação na prole de ratas Wistar prenhes

    Directory of Open Access Journals (Sweden)

    Vinicius von Diemen

    2010-02-01

    Full Text Available PURPOSE: Determine the effects of the MSG (monosodium glutamate in the offspring of pregnant rats through the comparison of the weight, NAL (nasal-anal length and IL (Index of Lee at birth and with 21 days of life. METHODS: Pregnant Wistar rats and their offspring were divided into 3 groups: GC, G10 and G20. Each of the groups received 0%, 10% and 20% of MSG, respectively from coupling until the end of the weaning period. RESULTS: Neither weight nor NAL were different among the groups at birth. The group G20 at birth had an IL lower than the group GC (pOBJETIVO: Avaliar o efeito do glutamato monossódico (GMS nos fetos de ratas prenhes por meio da comparação do peso, comprimento nasal-anal (CNA e índice de Lee (IL ao nascimento e com 21 dias de vida. MÉTODOS: Foram utilizadas ratas prenhes da linhagem Wistar distribuídas em três grupos: grupo controle (GC, G10 e G20. Estes, respectivamente, foram alimentados com ração contendo 0, 10 e 20% de GMS desde o período de acasalamento até o final da amamentação. RESULTADOS: O peso e o CNA não foram diferentes entre os grupos ao nascimento. O grupo G20, ao nascimento, teve IL menor que o grupo GC (p < 0,05 e, aos 21 dias de vida, apresentou peso e CNA menores que o grupo G10, o qual foi menor que o GC (p < 0,01. O grupo G20, aos 21 dias de vida, teve IL semelhante aos outros dois grupos. O percentual de ganho de peso do nascimento ao 21º dia de vida foi menor no G20 em relação aos outros dois grupos (p < 0,01. O grupo G20 teve percentual de aumento de CNA do nascimento ao 21º dia de vida menor que o grupo G10, e este menor que o grupo GC (p < 0,01. CONCLUSÕES: O GMS nas concentrações de 10 e 20% na ração de ratas prenhes Wistar apresentou uma relação dose-dependente nas variáveis peso e CNA. Houve diminuição no padrão de ganho de peso e de aumento de CNA do nascimento ao 21º dia de vida com uso de GMS. O IL na prole do grupo G20 aumentou em relação ao do grupo GC após 3

  9. O Efeito da crioterapia e compressão interminente no músculo lesado de ratos: uma análise morfométrica The effect of intermittent cryotherapy and compression on muscle injuries in rats: a morphometric analysis

    Directory of Open Access Journals (Sweden)

    NML Oliveira

    2007-10-01

    Full Text Available INTRODUÇÃO: Embora a crioterapia associada à compressão seja recomendada como tratamento imediato após lesão muscular, o efeito de sessões intermitentes desses procedimentos na área de lesão muscular secundária não é bem estabelecido. O objetivo deste estudo foi avaliar o efeito da aplicação intermitente de crioterapia e compressão (três sessões de 30 min a cada 1h30min na área de lesão do músculo tibialis anterior direito (TAD do rato. METODOLOGIA: A lesão muscular foi induzida por criolesão no TAD. Vinte e quatro ratos Wistar (340 ± 20g foram divididos em quatro grupos experimentais: a O grupo Lesão + Crioterapia (L+C recebeu tratamentos intermitentes de crioterapia e compressão; b O grupo Lesão + Placebo (L+P recebeu tratamento placebo; c O grupo Lesão (L não foi submetido a nenhum protocolo de tratamento; e d o grupo Crioterapia (C que permaneceu intacto e foi submetido a tratamentos de crioterapia e compressão. Os animais foram sacrificados 24h pós-lesão, sendo os músculos seccionados em criostato e os cortes histológicos corados com Azul de Toluidina para posterior mensuração da área muscular lesada (morfometria. A análise estatística constou da ANOVA e do teste Tukey (p INTRODUCTION: Although cryotherapy associated with compression has been recommended as an immediate treatment for muscle injuries, the effect of intermittent sessions of these procedures in the area of secondary muscle injuries has not been clearly established. OBJECTIVE: To evaluate the effect of intermittently applying cryotherapy and compression (three 30-minute sessions at 90-minute intervals on an injured area of the right tibialis anterior (RTA muscle in rats. Method: An injury was induced in the RTA muscle by means of cryoinjury. Twenty-four Wistar rats (340 ± 20g were divided into four experimental groups: a Injury + Cryotherapy (I+C, which received intermittent cryotherapy and compression; b Injury + Placebo (I+P, which

  10. Evaluation of the efficiency of a tubular digester in the reduction of organic load of biogas from swine wastes; Avaliacao da eficiencia de um biodigestor tubular na reducao da carga organica de biogas a partir de dejetos de suinos

    Energy Technology Data Exchange (ETDEWEB)

    Angonese, Andre Ricardo [Universidade Estadual do Oeste do Parana (UNIOESTE), PR (Brazil)], Email: aangonese@yahoo.com.br; Campos, Alessandro Torres [Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, MG (Brazil)], Email: atcampos3@yahoo.com.br; Moreno Palacio, Soraya [Universidade Estadual de Maringa (UEM), PR (Brazil); Szymanski, Nayara [Universidade Estadual do Oeste do Parana (UNIOESTE), PR (Brazil). Curso de Quimica

    2006-07-01

    The purpose of this study was to assess the efficiency of the anaerobic biological treatment systems in the reduction and stabilization of biodegradable organic matter of swine waste. The experiment was carried out at Vale dos Ipes Farm, located in the city of Ouro Verde do Oeste, in the Western of Parana State. One finishing phase swine unity containing 600 animals was monitored from January to June 2005. The system is composed by one steel digester with capacity for 50 m{sup 3}. The swine barn cleaning is performed by dry scratching on a daily basis. The generated residues flow by gravitation through ducts towards the digester. The duration of the hydraulic retention period was 12 days. The residues analysis was performed by means of sampling at the entrance and way out of the digester. The following parameters were analyzed: pH, DBO{sub 5}, DQO, total solids, total volatile solids, total fixed solids, total nitrogen e ammonia, potassium, total phosphate, average of biogas production. The results suggested that the anaerobic biological treatment system was efficient for reducing and stabilizing the organic matter resulted from the swine wastes. Expressive reductions of DBO, DQO, ST and SVT of 76, 77, 43 and 59% respectively, were obtained for the effluent originated by the digester. The average daily production of biogas during the analyzed period was 31, 5 m{sup 3}. (author)

  11. Evaluation of the effects of gamma radiation in minimally processed vegetables of Brassica oleracea species; Avaliacao dos efeitos da radiacao gama em vegetais da especie Brassica oleracea minimamente processados

    Energy Technology Data Exchange (ETDEWEB)

    Nunes, Thaise Cristine Fernandes

    2009-07-01

    The consumption of collard greens (Brassica oleracea cv. acephala) and broccoli (Brassica oleracea L. var. italica) has been inversely associated with morbidity and mortality caused by degenerative diseases. These species are highly consumed in Brazil, which enables its use as minimally processed (MP). The growing worldwide concern with the storage, nutritional quality and microbiological safety of food has led to many studies aimed at microbiological analysis, vitamin and shelf life. To improve the quality of these products, radiation processing can be effective in maintaining the quality of the product, rather compromising their nutritional values and sensory. The aim of this study was to evaluate the effectiveness of gamma radiation from {sup 60}Co at doses of 0, 1.0 and 1.5 kGy on the reduction of microbiota in these plants, and analyze their nutritional and sensory characteristics. The methodology used in this study was microbiological analysis, colorimetric analysis, analysis of phenolic compounds, antioxidant analysis and sensory analysis. The microbiological analysis showed a decrease in the development of populations of aerobic microorganisms, psychotropic and yeast and mold with increasing doses of radiation. The sensory analysis showed no significant difference between different times of cooking analyzed. The analysis of phenolic compounds, significant differences between the samples, suggesting that with increasing dose of irradiation was an increase in the amount of phenolic compounds found in broccoli and collard greens MP. It can be observed that the sample of control collard greens showed high antioxidant activity and for the samples treated by irradiation was a decrease of percentage. In contrast the samples of broccoli show an increase in the rate of scavenging DPPH with increase of the dose of radiation. The colorimetric analysis revealed that for samples of MP collard greens and broccoli foil of no significant differences, but for samples of stems of broccoli significant difference on the yellowing during storage. It is concluded that the processing of collard greens and broccoli butter by gamma radiation may be a viable alternative to the industry, since there was a reduction of the population of microorganisms, without changes in the sensory qualities and with minimum changes in the characteristics that confer antioxidant power. (author)

  12. Evaluation of toxicity and removal of color in textile effluent treated with electron beam; Avaliacao da toxicidade e remocao da cor de um efluente textil tratado com feixe de eletrons

    Energy Technology Data Exchange (ETDEWEB)

    Morais, Aline Viana de

    2015-07-01

    The textile industry is among the main activities Brazil, being relevant in number of jobs, quantity and diversity of products and mainly by the volume of water used in industrial processes and effluent generation. These effluents are complex mixtures which are characterized by the presence of dyes, surfactants, metal sequestering agents, salts and other potentially toxic chemicals for the aquatic biota. Considering the lack of adequate waste management to these treatments, new technologies are essential in highlighting the advanced oxidation processes such as ionizing radiation electron beam. This study includes the preparation of a standard textile effluent chemical laboratory and its treatment by electron beam from electron accelerator in order to reduce the toxicity and intense staining resulting from Cl. Blue 222 dye. The treatment caused a reduction in toxicity to exposed organisms with 34.55% efficiency for the Daphnia similis micro crustacean and 47.83% for Brachionus plicatilis rotifer at a dose of 2.5 kGy. The Vibrio fischeri bacteria obtained better results after treatment with a dose of 5 kGy showing 57.29% efficiency. Color reduction was greater than 90% at a dose of 2.5 kGy. This experiment has also carried out some preliminary tests on the sensitivity of the D. similis and V. fischeri organisms to exposure of some of the products used in this bleaching and dyeing and two water reuse simulations in new textile processing after the treating the effluent with electron beam. (author)

  13. Evaluation of synergistic effect in vacuum pack, refrigeration and irradiated treatments of minimally processed cassava; Avaliacao do efeito sinergistico da embalagem a vacuo, irradiacao e refrigeracao da mandioca minimamente processada

    Energy Technology Data Exchange (ETDEWEB)

    Pedroso, Bianca Maria

    2005-07-01

    Cassava is cultivated almost all over the world and it is considered one of the most important nutritious sources of calories in the human diet. Cassava is a viable food against starvation in several poor areas of the world because it is an extremely resistant culture and may reach satisfactory economical yield. We utilized vacuum packed industrialized cassava irradiated with 0,1 kGy, 3kGy and 5kGy and stored under refrigeration for 1, 21, 30 and 50 days. Our objective was to analyse the synergistic effect of vacuum packing, irradiation and refrigeration on the preservation of minimally processed cassava. The samples were analyzed for pH, acidity, weight, humidity, texture and color. The irradiation did not affect the chemical characteristics of the cassava. Neither the pH nor the acidity, the most relevant variables to verify deterioration in cassava, presented significant alterations during the period of storage. Comparing the irradiated treatments, the dose of 1kGy and 3kGy affected the physic-chemical characteristics of the cassava the least during the period of storage and refrigeration for 50 days; the doses of 1kGy,3kGy and 5kGy scored the highest rates the sensorial analysis during the period of storage for 21 days. (author)

  14. Social environmental and economic evaluation diagnosis through application of MADSA (Social and Environmental Evaluation Matrix) customization; Diagnostico de desempenho socioambiental e economico por meio da customizacao da MADSA (Matriz para Avaliacao de Desempenho Socioambiental)

    Energy Technology Data Exchange (ETDEWEB)

    Ziliotto, Marco Aurelio B.; Villa, Alessandra T.; Padilha, Simone L. Vieira; Canaverde, Patricia Margue [Instituto ECOPLAN, General Carneiro, PR (Brazil); Sanqueta, Carlos Roberto [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil)

    2004-07-01

    The present paper reports a social, environment and economic diagnosis in an oil and energy organization, in the enlargement of its plant. Attending to some previous requirements made by this company, the diagnosis was accomplished making use of an innovative evaluation tool called MADSA{sup R} (Social and Environmental Evaluation Matrix). Through the customization of this tool, it was possible to evaluate, systematically, the performance of the engaged companies hired by the organization to implement its enterprise, based on proposed actions in QSMS - Quality, Safety, Environment and Health. The performance evaluation was supported by the employees', the organization, its clients' and the local community perception. MADSA{sup R} methodology made it possible to measure the accomplished gain through proposed actions. The development of a performance indicator allowed the comparison between achieved results and accomplished investments. It was concluded that MADSA{sup R} provided subsidies to elaborate an action plan to improve the organization achievements, since it was able to represent the strategy of the organization, to guide and standardize actions, to identify and quantify points to be made better of. (author)

  15. Evaluation of the potentiality of the use of high-carbon microsilica as a pozzolanic material; Avaliacao da potencialidade da utilizacao de microssilica de alto teor de carbono como material pozolanico

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, R.L.S.; Pederneiras, C.M.; Costa, T.C.S.; Silva, C.H.R.B., E-mail: ruan_landolfo@hotmail.com [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil); Anjos, M.A.S. [Instituto Federal da Paraiba (IFPB), PB (Brazil); Nobrega, A.K. [Universidade Federal Rural do Semi-Arido (UFERSA), Mossoro, RN (Brazil)

    2016-07-01

    Supplementary Cementitious Materials reduce the production of clinker, which minimizes the environmental impact of cement production and the generation of industrial waste, also improve mechanical behavior and durability. Thus, this article aims to evaluate the potential use of microsilica with high content of carbon as pozzolanic material, based on the requirements of ISO 12653 (ABNT, 2015). The techniques of X-ray fluorescence (XRF), X-ray diffraction (XRD) and compressive strength of mortar of lime and cement (at 7 and 28 days, respectively) were used to evaluate the pozzolanic of the microsilica as a mineral addition. The results indicated that the microsilica has a high amorphous silicon dioxide percentage structure. In the DRX could be possible to analyze the formation of CSH, justifying the good results of mechanical strength, especially with cement. Thus, the mineral admixture used in this research can be considered as a pozzolanic material. (author)

  16. Calculation of the uncertainty of H{sub P} (10) evaluation for a thermoluminescent dosimetry system; Calculo da incerteza da avaliacao do H{sub P} (10) para um sistema de dosimetria termoluminescente

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, M.S.; Silva, E.R.; Mauricio, C.L.P., E-mail: max.das.ferreira@gmail.com [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2016-07-01

    Full interpretation of dose assessment only can be performed when the uncertainty of the measurement is known. The aim of this study is to calculate the uncertainty of the TL dosimetry system of the LDF/IRD for evaluation of H{sub P} (10) for photons. It has been done by experimental measurements, extraction of information from documents and calculation of uncertainties based on ISO GUM. Energy and angular dependence is the most important source to the combined u{sub c}(y) and expanded (U) uncertainty. For 10 mSv, it was obtained u{sub c}(y) = 1,99 mSv and U = 3,98 mSv for 95% of coverage interval. (author)

  17. Evaluation of oil and grease removal by adsorptive polymeric resins in semi-industrial scale: influence of temperature; Avaliacao da remocao de oleos e graxas por resinas polimericas adsorventes em escala semi-industrial: influencia da temperatura

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Luis F.S. de; Silva, Carla M.F. da; Queiros, Yure G.C.; Lucas, Elizabete F. [Universidade Federal do Rio de Janeiro, Instituto de Macromoleculas, Laboratorio de Macromoleculas e Coloides na Industria de Petroleo, Rio de Janeiro, RJ (Brazil)], e-mail: elucas@ima.ufrj.br

    2011-07-01

    The aim of this study was to evaluate the performance of polymeric resins packed in a fixed bed eluted in semi-industrial scale for oil and greases removal disposed in synthetic oily water in different temperature conditions. For this work, columns packed with vinyl and acryl polymer-base were tested and their efficiency of oil removal was evaluated by fluorimetry technique in two different temperatures: 25 and 60 deg C, in a flow rate condition of 200 mL/min. The experimental results were very good: the removal efficiencies were above 98% in both cases. At 60 deg C, the system keep the efficiency for a longer time: no significant loss in the efficiency was observed after eluting 1,000 times of the column bed volume at 25 deg C and 2,000, at 60 deg C. This result characterizes a great potential of application in the industry. (author)

  18. Resistance evaluation expanded perlite the leaching acid: variation of parameters concentration, time and leaching agent; Avaliacao da resistencia da perlita expandida a lixiviacao acida: variacao dos parametros concentracao, tempo e agente lixiviante

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, J.M.F. de; Damasceno Junior, E.; Oliveira, E.S.; Fernandes, N.S., E-mail: janielequimicaufrn@gmail.com [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Instituto de Quimica. Laboratorio de Quimica Analitica e Meio Ambiente

    2016-07-01

    The expanded perlite is an amorphous aluminosilicate which presents in its composition about 75.0% silicon oxide (SiO2), also having other species in the composition as oxides of some metals. Silicas and silicates have been used in the environmental field, in relevant anti-corrosive activity. In this context, materials that exposes too many highly acidic media, require preservation against this type of wear, as this type of damage causes a great financial loss, thereby requiring low-cost, abundant materials, non-toxic and easy to purchase as some silica coating. The study evaluated the perlite expanded resistance against an acid leaching process. With undeniability the use of strong acids and different working conditions were not able to remove the oxides present on the expanded perlite sample, thus demonstrating the high strength of the expanded perlite against acid attacks. (author)

  19. Evaluation of internal occupational exposure of workers from nuclear medicine services by aerosol analysis containing {sup 131}I; Avaliacao da exposicao interna de trabalhadores em servicos de medicina nuclear atraves da analise de aerossois contendo {sup 131}I

    Energy Technology Data Exchange (ETDEWEB)

    Carneiro, Luana Gomes; Sampaio, Camilla da Silva; Dantas, Ana Leticia Almeida; Lucena, Eder Augusto; Santos, Maristela Souza; Dantas, Bernardo Maranhao, E-mail: carneiro@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ),Rio de Janeiro, RJ (Brazil); Paula, Gustavo Affonso de [Escola SESC de Ensino Medio, Rio de Janeiro, RJ (Brazil)

    2014-07-01

    This study evaluated the risk of internal occupational exposure associated with the incorporation of {sup 131}I via inhalation, in Nuclear Medicine Services, using aerosol analysis techniques. Occupationally Exposed Individuals (IOE) involved in handling this radionuclide are subject to chronic exposure, which can lead to an increase in the committed effective dose. Results obtained in preliminary studies indicate the occurrence of incorporation of {sup 131}I by workers involved in handling solutions for radioiodine therapy procedures. The evaluation was carried out in radiopharmacy lab (nuclear medicine service) of a public hospital located in the city of Rio de Janeiro. After confirmed the presence of the radioisotope, by a qualitative assessment, it was determined an experimental arrangement for sample collection and were detected and quantitated the presence of steam {sup 131}I during routine work. The average concentration of activity obtained in this study was 3 Bq / m{sup 3}. This value is below of Derived Concentration in Air (DCA) of 8.4 x 10{sup 3} Bq of {sup 131}I / m{sup 3} corresponding to a committed effective dose of 1.76 x 10{sup -4} mSv. These results demonstrate that the studied area is safe in terms of internal exposure of workers. However, the presence of {sup 131}I should be periodically reevaluated, since this type of exposure contributes to the increase of the individual effective doses. Based on the data obtained improvements were suggested in the exhaust system and the use of good work practices in order to optimize the exposures.

  20. Preliminary analysis of doses to evaluate the image quality in radiographic examinations in veterinary radiology;Analise preliminar das doses para avaliacao da qualidade da imagem em exames radiograficos na radiologia veterinaria

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, Ana Carolina B.C.F.; Dias, Mayara T.P.; Santos, Andrea C. [Universidade de Sao Paulo (USP), SP (Brazil). Faculdade de Medicina Veterinaria e Zootecnia; Melo, Camila S.; Furquim, Tania A.C. [Universidade de Sao Paulo (IEE/USP), SP (Brazil). Inst. de Eletrotecnica e Energia

    2009-07-01

    This work has as objective to promote the analysis of the radiological doses and quality of the image of the technical letter used for the accomplishment of thorax and coxal radiographic examination of animals of canine and feline species. The study was accomplished in the service of Diagnosis for Image in Veterinarian Hospital of Veterinary Medicine and Zootecnia College of University of Sao Paulo, in two conventional equipment. Initially, physical features of the animals and the technique used were collected for each one of the 188 radiographic examinations of thorax and 52 examinations of coxal. The animals were placed in different groups, according to their body weight. For each group, the averages for each feature were calculated: thickness of the radiographed region, tension, electric current, time of exhibition, current product electric-time, size of the used film, presence or absence of bucky and feature of focus (narrow or thick). On the basis of the averages of group M (of lesser weights that 5kg for cats and between 10,1kg and 20kg for dogs), was executed a physical analysis of the current technical letter, using the equipment: ionization chamber (to determinate the value of kerma in air), simulator objects (representative of the thickness of the animal) and three dispositive standards of test that evaluate space resolution, resolution in low contrast and contrast-detail. The obtained images were analyzed and compared for a physicist and a radiologist medical veterinary. The results had shown that the examinations supply dose considered high for techniques used mainly for coxal. The equipment A, although to supply higher doses, presents the better images for the majority of the projections. However, the study indicates that there are not exactly reference levels, but these examinations must pass for improvement of quality of image (author)

  1. In vitro assessment of the composition and microhardness of hard tissues of oral cavity submitted to gamma irradiation; Avaliacao in vitro da composicao e microdureza dos tecidos duros da cavidade bucal submetidos a irradiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Paredes, Wilber Edison Bernaola

    2017-11-01

    Clinical Radiotherapy is extremely important for the treatment of malignant lesions of the head and neck region, however, exposure to ionizing radiation can lead to systemic or local complications during and after radiation treatment. Among these immediate local complications are the oral cavity xerostomia and the consequent oral mucositis. Regarding late complications produced by radiation, tooth decay of radiation and osteoradionecrosis are included, which are considered dose-dependent lesions, with high incidence in recent decades and difficult to manage, although these appear after completion of treatment and under the influence of local factors. The methodology proposed in this study consists in evaluating the effect of gamma radiation after irradiation of the samples, using the dose used in patients suffering with head and neck cancer. The samples were obtained from human enamel and root dentin; and swine mandibular bone, which were previously polished, and then submitted to the analysis of the initial surface microhardness of all groups. Subsequently, the samples were irradiated in a dose rate of 4 Gy per day, completing a total dose of 72 Gy. Finally, the samples were submitted to surface microhardness analysis after irradiation, which presented statistically significant results from the Student t, ANOVA and Tukey statistical tests referred to the difference of the mean of the initial and final values of each study group with a significant value of p = 0.00 (<0.05). Regarding the morphological analysis in scanning electron microscopy (SEM), the deleterious effect of gamma irradiation was evidenced as structural cracks, breaks and superficial fractures of the analyzed tissues and the biochemical analysis by Attenuated Total Reflection technique using Fourier transform infrared spectroscopy (ATR - FTIR) showed degradation of inorganic components and denaturation of organic compounds; whereby, the effect of gamma irradiation on the hard tissues of the oral cavity with respect to mechanical, compositional and morphological properties was deleterious and this study highlighted the isolated action of this ionizing radiation which contributes highly for its appearance, independent of local and systemic factors in the irradiated patients. (author)

  2. The refractometry use in the evaluation of type 'C' gasoline non-conformities; Uso da refratometria na avaliacao de nao conformidades da gasolina tipo 'C'

    Energy Technology Data Exchange (ETDEWEB)

    Santos, K.M.; Andrade, J.M.; Severiano, M.L.; Medeiros, M.A.O.; Fernandes, N.S.; Fernandes, V.J. [Rio Grande do Norte Univ., Natal, RN (Brazil)]. E-mail: klecia.morais@bol.com.br

    2003-07-01

    The need of the creation of practical and efficient methods that can monitor the quality of the automotive fuels it is an important factor, because they should evaluate the physical-chemical properties of the fuels to guarantee your conformity. To determine these parameters of conformity, several regulars analyses are done in the gasoline type 'C', as the distillation and content of ethyl alcohol (AEAC). However, the present work has as objective uses the refractometry in the forecast of irregularities in the gasoline trying to establish a faster and efficient analysis method. (author)

  3. Evaluation of the effects resulting from the application of magnetic fields on the properties of crude oils and paraffins; Avaliacao da influencia da aplicacao de campos magneticos sobre as propriedades de petroleo e parafinas

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Lenise Couto; Rocha, Nelson de Oliveira; Dittz, Carlos Henrique da Silva [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES). Setor de Tecnologia de Processamento e Transporte

    2000-03-01

    In petroleum industry the phenomenon of build-up of organic deposits, basically of paraffinic nature, into surface equipment, flow lines and columns has been one of the greatest problems concerning the production and transfer of crude oils, leading to significant and increasing losses in production. The approach through the application of magnetic field is a novel technique, neither explained nor exploited altogether, not one that turns out to be a simple preventive solution for the problem requiring low investment and showing expressive benefits, provided its effectiveness is confirmed. The main results of laboratory test where the effects of application of magnetic fields on the properties of two crude oil types, one mixture of paraffins and two reference solution were evaluated are presented herein, and this paper also approaches the influence of process variables (Magnetization temperature, exposure time and magnetic field density) on the efficiency of this process of treatment. (author)

  4. Evaluation of the mineralogical characterization of several smectite clay deposits of the state of Paraiba, Brazil using statistical analysis of variance; Avaliacao da caracterizacao mineralogica de diversos depositos de argilas esmectiticas do estado da Paraiba utilizando analise estatistica de variancia

    Energy Technology Data Exchange (ETDEWEB)

    Gama, A.J.A.; Menezes, R.R.; Neves, G.A.; Brito, A.L.F. de, E-mail: agama@reitoria.ufcg.edu.br [Universidade Federal de Campina Grande (UFCG), PB (Brazil)

    2015-07-01

    Currently over 80% of industrialized bentonite clay produced in Brazil in sodium form for use in various industrial applications come from the deposits in Boa Vista - PB. Recently they were discovered new bentonite deposits situated in the municipalities of Cubati - PB, Drawn Stone - PB, Sossego - PB, and last in olive groves - PB, requiring systematic studies to develop all its industrial potential. Therefore, this study aimed to evaluate chemical characterization several deposits of smectite clays from various regions of the state of Paraíba through the analysis of statistical variance. Chemical analysis form determined by fluorescence x-ray (EDX). Then analyzes were carried out of variance statistics and Tukey test using the statistical soft MINITAB® 17.0. The results showed that the chemical composition of bentonite clay of new deposits showed different amounts of silica, aluminum, magnesium and calcium in relation clays in Boa Vista, and clays imported. (author)

  5. Model for qualitative evaluation of risk in ducts through the fuzzy logic in conformity with the methodology of IBR; Modelo para avaliacao qualitativa do risco em oleodutos atraves da logica fuzzy segundo a metodologia da IBR

    Energy Technology Data Exchange (ETDEWEB)

    Mishina, Koje Daniel Vasconcelos [Centro Federal de Educacao Tecnologica da Bahia (CEFET-BA), Salvador, BA (Brazil); Silva, Jose Felicio da; Silva, Joao Bosco de Aquino [Universidade Federal da Paraiba (UFPb), Joao Pessoa, PB (Brazil)

    2006-07-01

    This work considers a system for qualitative evaluation of the terrestrial risk in pipelines, based in the methodology of the Inspection Based on Risk and the Fuzzy Logic. For this one developed a matrix of risk associated with the oil transport and its derivatives, that define the type of tracking and the regularity of inspection with Pig Instrumented in function of the level of risk found in the stretch in study. This matrix of risk considers the probabilities and the consequences associates to the damage for corrosion. The evaluation of the considered system was based on the consistency of the found levels of risk, in relation to that it would be found in the practical one. The gotten results had demonstrated that the use of the methodology of the IBR and the Fuzzy Logic can be used jointly as one sufficiently efficient alternative technique in the evaluation of risk in corroded pipe-lines. (author)

  6. Environmental analysis of the proton exchange membrane fuel cell on the subject of life cycle assessment; Analise ambiental da celula a combustivel de membrana trocadora de protons sob o enfoque da avaliacao do ciclo de vida

    Energy Technology Data Exchange (ETDEWEB)

    Fukurozaki, Sandra Harumi

    2006-07-01

    The energy is the fuel of growth and an essential requirement for the socioeconomic development. However, the current production model is based on fossil fuels, considered as threat to man and nature. As for, the relating to the human activities and their effects on the environment, they are handled by the implementation of a more rigid model of environmental control and the mobilization of the society in favor of technologies with less energy impact. In view of this scenario, the Proton Exchange Membrane Fuel Cell - PEMFC has been recognized as a key for the vital need of a clean and efficient energy. Considering the conventional power generation system, their advantages during usage configure its application as an ideal option for several utilities, especially in the mobile sector. Even though, the focus on several environmental evaluations in energy systems is referred back to the initial stage of it use, the employment relating to production of the system and to final destination should be considered, since these also present impacts. In the case of PEMFC, their previous and subsequent phases of use are issues related to the platinum catalysts, which indicates an environmental importance that cannot be overlooked. In this sense, the Life Cycle Assessment has been used to understand and to question the risks and opportunities that are associated to certain product, starting from a systemic concept of their relationships with the environment. It is precisely in this context that the present research intends to present its major contribution, starting from an exploratory study towards the its objectives to provide an environmental analysis of such technology linked to post stage of powder-use of the membrane electrode assembly - MEA, concerning the platinum catalysts, on the subject of Life Cycle Assessment - LCA. To attain such aim, the relationships between energy, environment and development are presented and discussed, as well as, the Fuel Cell technology and the current studies on LCA of PEMFC. Several questions raised up on this issues have contributed in the development of a method of recuperating the PEMFC catalysts and, particularly, for its subsequent environmental evaluation. Among significant results are the importance of LCA, out lined as useful tool for perceiving the weight of environmental matters concerning the platinum and its subsidy strategies relating to the development, consolidation and to the innovation of PEMFC. (author)

  7. Inorganic composition determination and evaluation of the biological activity of Peperomia pellucida in the Aspergillus flavus growth; Estudo da composicao inorganica e avaliacao da atividade biologica de Peperomia pellucida no crescimento de Aspergillus flavus

    Energy Technology Data Exchange (ETDEWEB)

    Sussa, Fabio Vitorio

    2011-07-01

    In recent decades, there has been a great advancement in research in developing drugs from plants. Nevertheless, little significant care exists in the literature about the stable concentration of elements, as well as the activity concentration of '2{sup 38}U and {sup 232}Th decay products in plants used for this purpose in Brazil. The presence of stable elements and radionuclides in plants constitutes the pathway for their migration to humans, via uptake of tea or remedies made with medicinal herbs. Peperomia pellucida, whose popular name is 'erva de jabuti', is a plant known by its medicinal usages, such as healing and analgesic properties plus antibacterial and antifungal activities against food fungi. In this study, the elemental composition of Peperomia pellucida and surrounding soil samples collected in the Botanical Garden in Rio de Janeiro was determined; the elemental concentration in the alcoholic extract and infusion processes of dry plants was also determined. The elemental concentration of As, Ba, Br, Ce, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Na, Nd, Rb, Sb, Sc, Sm, Ta, Tb, Th, U, Yb and Zn was determined in the leaves, aerial parts, including leaves, stems and roots of Peperomia pellucida, in their extracts obtained by maceration and infusions and in the surrounding soil by the Instrumental Neutron Activation Analysis (INAA). The analytical methodology used to determine the elements Cd, Hg and Pb was the Atomic Absorption Spectrometry (AAS); the activities of {sup 226}Ra, {sup 228}Ra and {sup 210}Pb were carried out by gross alpha and beta counting, after radiochemical separation. The quality of the obtained results was assured by the analyses of the certified reference materials IAEA-336 Lichen, IAEA-Soil-7, IAEA-326 Radionuclides in soil, NIST 1515 Apple Leaves and NIST 1542 Peach Leaves. The relative standard deviations and the relative errors obtained in these analyses indicated good precision and accuracy of the results. The essential oil, ethanolic and hexane extracts of Peperomia pellucida were tested for antifungal activity against Aspergillus flavus in vitro on Petri plates. The antifungal activity was based on the inhibition zone and IC{sub 50} values against the pathogen on Petri plates assays. Also, the essential oil chemical composition was determined by GC-MS. (author)

  8. Assessment of atmospheric pollution of chemical elements by epiphytic lichen analysis at the Campus of the Sao Paulo University; Avaliacao da poluicao atmosferica de elementos quimicos pela analise de liquen epifitico no Campus da Cidade Universitaria de Sao Paulo

    Energy Technology Data Exchange (ETDEWEB)

    Rocha, Rosiana Rocho

    2015-07-01

    Air pollution has been a frequent topic of research, due to the effects that it can cause on the health of living organisms, environment and climate. In order to identify pollution sources and their effects, biomonitoring has been studied due to its low cost and possibility of sampling in wide geographic areas. In this study for passive biomonitoring of air pollution levels at the Cidade Universitaria Armando Salles de Oliveira (CUASO), University of Sao Paulo campus, epiphytic lichens of Canoparmelia texana species were used. The lichens collected from tree barks at different sampling sites in the CUASO were cleaned, freeze-dried and ground for analyses. Lichen samples were analyzed by X - ray fluorescence spectrometry (XRFS) and neutron activation analysis (NAA). For XRFS, cylindrical pellets of samples were prepared to determine As, Br, Ca, Cl, Cr, Cu, Fe, K, Mn, Rb, S, Sr and Zn. For NAA, lichen sample aliquots along with synthetic elemental standards were irradiated both for short and long periods at the IEA-R1 nuclear research reactor. The induced activities were measured by a gamma ray spectrometer to determine As, Br, Ca Cd, Cl, Co, Cr, Cs, Cu, Fe, K, La, Mg, Mn, Rb, Sb, Sc, Se, U, V and Zn. The precision and accuracy of the results were evaluated by the analysis of certified reference materials (MRCs). Their results of relative errors and standard deviations were below 15% for most of the elements. The standardized difference or En score values were lower than |1| indicating satisfactory results. Replicate analyses of a lichen sample by XRFS and NAA, indicated good homogeneity of the sample for the elements determined. The lichen results showed that the mean concentrations of As, Br, Ca, Cd, Co, Cr, Cs, Rb, Sb, Se and U were higher in samples from CUASO than those from regions considered unpolluted. For Fe, K, La, S, V and Zn, they were of the same order of magnitude. The correlation study between the elements showed high correlation (r > 0.7) for elements originated from the natural and anthropogenic sources. The principal component analysis (PCA) applied to the results showed six major components, where components 1 and 2 accounted for 52.9 % of the variance of the data. Enrichment factors (EF) calculated for the results of lichens from CUASO and a region considered unpolluted were EF> 1 for most elements, showing that these elements may be from anthropogenic sources. From the results obtained in this study can be concluded that the elements found in lichens from CUASO can be originated from sources such as resuspension of soil particles and anthropogenic emissions. (author)

  9. Evaluation of image quality versus doses in service of mammography of Belo Horizonte, MG, BR; Avaliacao da qualidade da imagem versus doses em servicos de mamografia de Belo Horizonte

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, M.; Nogueira, M.S.; Guedes, E. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil). Seguranca Nuclear; Andrade, M.C. [Superintendencia Estadual de Vigilancia Sanitaria, Belo Horizonte, MG (Brazil); Peixoto, J.E. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Radiologia; Silva, V.L.S.; Borges, J.C. [Vigilancia Sanitaria da Secretaria Municipal de Saude de Belo horizonte (VISA/PBH), MG (Brazil)

    2004-07-01

    Cancer is the second cause of death for Brazilian women and breast cancer is the most common neoplasm amongst women. Mammography is an essential tool for diagnosis and early detection of this disease. In order to be effective, the mammography must be of good quality. This study sought to evaluate the radiation protection and quality of 37 services out a total of 82 mammography facilities existing in the city of Belo Horizonte, Brazil. Two instruments were used: a proposed evaluation protocol of the Health Surveillance -VISA and a protocol for evaluation of image quality. Of the 37 services who completed the study, none was achieved 100% conformity in image quality. The results of radiation protection requirements according to the VISA protocol bore close relation to final image quality, a central issue for early cancer detection (p>0.05)

  10. Evaluation of {sup 137}Cs mobility in soil profiles from the Pantanal region, Brazil; Avaliacao da mobilidade do {sup 137}Cs em perfis de solos da regiao do Pantanal

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Fernanda Leite da

    2013-07-01

    Radioactive pollutants can cause impact on the environmental quality of soils and pose a risk to human health. The release of radioactive materials through nuclear testing or nuclear accidents cause the deposition of radionuclides on the ground,· it may be leached by rain, transported to the sources of natural waters and absorbed by the soil fauna and flora, and thus enter the human food chain. Radioecological studies have shown that soils with low pH, low organic matter content and low fertility are more vulnerable to contamination by {sup 137}Cs, since the transfer to plants is high. In this study, we aimed to assess and map the vulnerability to contamination by {sup 137}Cs surface horizons of the soils from the Pantanal and propose mitigation measures adapted to the regional scenario to optimize radiological protection of agricultural areas. Therefore, selected soil profiles located in the municipality of Jaraguari, state of Mato Grosso do Sul, and applied the conceptual model developed by Picanco Jr (2012), which was used in the reference values of the factor of soil-plant transfer (FT) for {sup 137}Cs in corn, related soil variables (pH, CTC and exchangeable K) and relevance of parameters and variations of amplitudes for each value range of FT. The application of this conceptual model established to detect the vulnerability of soils to radioactive contamination generated maps vulnerability showing that the region is very heterogeneous as this criterion, showing low levels of vulnerability for most of the region and in some areas, extreme vulnerability. This result identified the Pantanal as one of the less vulnerable to the radioactive contamination, but the sparse areas of extreme vulnerability can lead to contamination of subsoil and a significant spread of contamination via groundwater. This conceptual model, which defines vulnerability classification, is a first step for the study and determination of a numerical index of vulnerability to {sup 137}Cs soil and can be used in the task of remediation in case of rural areas by accident and {sup 137}Cs contamination establishing geographic areas where we should prioritize treatment due to a greater vulnerability. (author)

  11. Evaluation gamma radiation in composite sisal fiber- polyurethane derived of castor oil by bending test; Avaliacao da influencia da radiacao gama em compositos de fibra de sisal - poliuretano derivado de oleo de mamona atraves de ensaios de flexao

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Felipe H. de; Geraldo, Ricardo R.; Vasco, Marina C.; Azevedo, Elaine, E-mail: helunica@yahoo.com.br [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Claro Neto, Salvador [Universidade de Sao Paulo (USP), Sao Carlos, SP (Brazil). Instituto de Quimica

    2015-07-01

    Materials used for making furniture and accessories or positioning in X -ray examination rooms should not exhale volatile organic compounds and are resistant to ionizing radiation. One solution is the use of vegetable fiber and polyurethane composites of vegetable origin, since they are biodegradable, derived from renewable raw materials and have no volatile organic compounds. The main difficulty in developing this material is fiber adhesion with the polymer. The objective of this study is to evaluate the mechanical properties of composite sisal fiber composite, without further treatment, and polyurethane derived from castor oil, with a dose of 25 kGy gamma radiation, subjected to 3 points bending tests. (author)

  12. Evaluation exposure to the ionizing radiation of workers during the operation of first Brazilian uranium mine; Avaliacao da exposicao de trabalhadores a radiacao ionizante durante a operacao da primeira mina de uranio do Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Wagner de S.; Oliveira, Sergio Q. de; Py Junior, Delcy de A.; Dantas, Marcelino V.A.; Silva, Ana Claudia A.; Garcia Filho, Oswaldo, E-mail: wspereira@inb.gov.b, E-mail: sergioquinet@inb.gov.b, E-mail: delcy@inb.gov.b, E-mail: marcelino@inb.gov.b, E-mail: anasilva@inb.gov.b, E-mail: ogarcia@inb.gov.b [Industrias Nucleares do Brasil (UTM/INB), Pocos de Caldas, MG (Brazil). Unidade de Tratamento de Minerios. Servico de Radioprotecao

    2011-10-26

    The production of uranium in Brazil initiated, in industrial scale, in the year of 1982, in the Miner-Industrial Complex at Pocos de Caldas, Minas Gerais, Brazil. This paper analysed the data of 13410 individual historic of dose, registered along the years of work, bay the radioprotection service of installation. Descriptive statistics and frequency histograms based on dose historic, and the annual distribution doses and the accumulated doses were evaluated during the functioning period of the CIPC, and his adequacy to the in force legislation at the time of it operation

  13. Profit of solar energy for salting and drying of a brazilian fish Colossoma mitrei Berg, 1895; Utilizacao da energia solar para avaliacao da salga e secagem do pacu, Colossoma mitrei Berg, 1895

    Energy Technology Data Exchange (ETDEWEB)

    Sales, Ronaldo de Oliveira [1Ceara Univ., Fortaleza, CE (Brazil). Centro de Ciencias Agrarias; Beirao, Luiz Henrique [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Ciencias e Tecnologia de Alimentos

    1986-12-01

    This paper studied a simple method for processing dried-salted `pacu` fish. The average weight in relation to total weight was 58,6% and the result came very close to the average of weight of other dried salted fishes, when treated in same way. The main purpose of this process was to reduce the long time expanded by the conventional process, allowing to reduce the humidity of the dried-salted product from 56 to 43,5% in 28 hours only. (author) 20 refs., 3 figs., 8 tabs.

  14. Evaluation of the influence of water and oil derivatives absorption on PVC pipes; Avaliacao da influencia da absorcao de agua e de derivados de petroleo em tubulacoes de PVC

    Energy Technology Data Exchange (ETDEWEB)

    Carpio, D.C.F. del; D' Almeida, J.R.M., E-mail: dalmeida@puc-rio.b [Pontificia Univ. Catolica do Rio de Janeiro (PUC-Rio), RJ (Brazil). Dept. de Engenharia de Materiais

    2010-07-01

    PVC is the only polymer of large consume that is not totally obtained from petroleum, since it contains 57% of chlorine. As chlorine containing materials are resistant to bacteria rich environments, such as buried pipes, PVC is being used for fluid transportation, principally water, but it can also be considered as an alternative material for the transportation of other fluids. This work analyzes the aging behavior of PVC exposed to water, ethanol and diesel oil, using TGA, DSC, FT-IR and DR-X techniques. The results showed that