WorldWideScience

Sample records for rapidly developing asian

  1. A STUDY ON THE INTRODUCTION OF BUS RAPID TRANSIT SYSTEM IN ASIAN DEVELOPING CITIES

    Directory of Open Access Journals (Sweden)

    Thaned SATIENNAM

    2006-01-01

    Full Text Available Bus Rapid Transit (BRT has increasingly become an attractive urban transit alternative in many Asian developing cities due to its cost-effective and flexible implementation. However, it still seems to be difficult to introduce BRT to these cities because almost all of their city structures have been developed under solely a road transport development city plan and weakness of land use control gives rise to many problems, such as urban sprawl, traffic congestion, and air pollution. The purpose of this study was to introduce several strategies to support BRT implementation in Asian developing cities, such as a strategy to appropriately integrate the paratransit system into BRT system as being a feeder along a BRT corridor to supply demand. These proposed strategies were evaluated by applying demand forecasting and emission models to the BRT project plan of Bangkok Metropolitan Administration (BMA in Thailand. It was demonstrated that the proposed strategies could effectively improve the BRT ridership, traffic conditions, and air pollution emission of the entire system in Bangkok. This study could be further extended to include strategy recommendation if a BRT system were to be introduced to other Asian developing cities.

  2. Potential for modal shift by passenger car and motorcycle users towards Bus Rapid Transit (BRT in an Asian developing city

    Directory of Open Access Journals (Sweden)

    Thaned Satiennam

    2016-03-01

    Full Text Available Many developing Asian cities consider a Bus Rapid Transit (BRT system in their public transport planning because of its advantages, offering lower investment cost and flexible implementation over rail systems. The objective of this research is to assess the potential of BRT for attracting travelers from passenger cars and motorcycles. Three different BRT systems were designed for the main corridor passing through Khon Kaen City in Thailand. The study developed modal split models for predicting the choices of passenger car users and motorcycle users. The models were developed based on a Stated Preference (SP survey. It was found that BRT could attract significantly private vehicle users to change mode choice. The proportion of motorcycle users shifting is higher than passenger car users. However, the majority of private vehicle users still prefer their own private vehicles. Both travel time and travel cost affects the mode choice, in which travel time has a highly significant effect on passenger car users' choice of BRT, but travel cost has a highly significant effect on motorcyclists' choice. Some socio-economic factors, including gender, age, driving license holding and residence location also influence the choice of BRT.

  3. Rapid and specific detection of Asian- and African-lineage Zika viruses

    Science.gov (United States)

    Chotiwan, Nunya; Brewster, Connie D.; Magalhaes, Tereza; Weger-Lucarelli, James; Duggal, Nisha K.; Rückert, Claudia; Nguyen, Chilinh; Garcia Luna, Selene M.; Fauver, Joseph R.; Andre, Barb; Gray, Meg; Black, William C.; Kading, Rebekah C.; Ebel, Gregory D.; Kuan, Guillermina; Balmaseda, Angel; Jaenisch, Thomas; Marques, Ernesto T. A.; Brault, Aaron C.; Harris, Eva; Foy, Brian D.; Quackenbush, Sandra L.; Perera, Rushika; Rovnak, Joel

    2017-01-01

    Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. PMID:28469032

  4. Did China follow the East Asian development model?

    OpenAIRE

    Boltho, Andrea; Weber, Maria

    2009-01-01

    China is located in East Asia and, just as Japan, Taiwan or (South) Korea at earlier stages of their development, has now grown very rapidly for some three decades. That is not enough, however, for it to qualify for membership of the club. The East Asian development model has a number of additional and important characteristics. Four are selected for discussion: the almost constant encouragement given to investment, the manufacturing sector and external competitiveness, and pursued via a vari...

  5. Asian development bank: can it become an actual development bank?

    OpenAIRE

    Khan, Haider A.

    2012-01-01

    The Asian Development Bank(ADB) was founded in the mid-sixties. In addition to the Asian countries, it also has members from among the wealthy countries in North America, Europe and Oceania. Over the years, ADB has grown in size and prestige among the regional development banks. As Khan (2004, 2007 and 2012) argues, within a hybrid workable global financial architecture, regional development banks like the ADB can play a constructive role.

  6. Regional conditions in East Asian development

    DEFF Research Database (Denmark)

    Søborg, Henrik

    2006-01-01

    The present volume contains case studies of how government og firms in a number of Asian countries have responded to challenges of globalisation and how that has affected their economic transformation.......The present volume contains case studies of how government og firms in a number of Asian countries have responded to challenges of globalisation and how that has affected their economic transformation....

  7. Commentary: An Asian Americanist Perspective on Child Development.

    Science.gov (United States)

    Lee, Richard M; Y J Kim, Adam; Zhou, Xiang

    2016-07-01

    In this commentary, we put forth an Asian Americanist perspective on child development that frames, expands upon, and at times challenges the contextual, conceptual, and methodological ideas put forward by Kiang et al., Mistry et al., and Yoshikawa et al. (this volume). This Asian Americanist perspective draws upon scholarship in Asian American Studies and critical race theory to bridge the historical, conceptual, and methodological contributions of the three articles. We also aim to challenge current and future generations of scholars studying Asian American child development to look at Asian American youth and families as autonomous, self-determining agents who are capable of challenging, resisting, and affecting change in a racialized society. © 2016 The Authors. Child Development © 2016 Society for Research in Child Development, Inc.

  8. From Dependence to Autonomy. The Development of Asian Universities.

    Science.gov (United States)

    Altbach, Philip G., Ed.; Selvaratnam, Viswanathan, Ed.

    A collection of works on the development of Asian universities is presented, focusing on an aspect of higher education not previously analyzed: the contemporary impact of Western academic systems in Asia. Eleven papers fall into three sections following the introduction, "Twisted Roots: The Western Impact on Asian Higher Education," (P.…

  9. Growth, human development, and trade: the Asian experience

    OpenAIRE

    Mustafa,Ghulam; Rizov, Marian; Kernohan, David

    2017-01-01

    This study looks at the three-way relationship between economic growth, human development, and openness to trade in a large panel of developing Asian economies. Using a theoretically motivated simultaneous equations system, we find that although human development contributes positively to economic growth, in the case of our Asian sample growth does not appear to have had a positive influence on human development. Uneven growth accompanied by lagging institutional development, preventing human...

  10. Asian American Career Development: A Qualitative Analysis

    Science.gov (United States)

    Fouad, Nadya A.; Kantamneni, Neeta; Smothers, Melissa K.; Chen, Yung-Lung; Fitzpatrick, Mary; Terry, Sarah

    2008-01-01

    This study used a modified version of consensual qualitative research design to examine how contextual, cultural, and personal variables influence the career choices of a diverse group of 12 Asian Americans. Seven domains of influences on career choices emerged including family, culture, external factors, career goals, role models, work values,…

  11. Did China follow the East Asian development model?

    Directory of Open Access Journals (Sweden)

    Boltho, Andrea

    2009-12-01

    Full Text Available China is located in East Asia and, just as Japan, Taiwan or (South Korea at earlier stages of their development, has now grown very rapidly for some three decades. That is not enough, however, for it to qualify for membership of the club. The East Asian development model has a number of additional and important characteristics. Four are selected for discussion: the almost constant encouragement given to investment, the manufacturing sector and external competitiveness, and pursued via a variety of fairly interventionist industrial, trade and financial policies; a concomitant belief in the virtues of intense domestic (Japan and Taiwan and foreign (Korea competition; a set of broadly sensible and appropriate macroeconomic policies; and a number of favourable (pre-conditions, such as the presence of a homogeneous population, a relatively high stock of human capital, reasonable income equality and fairly authoritarian governments. China, since reforms began in the late 1970s, has shared some of these characteristics, but not all. In particular, it is still much more of a command economy than the other three countries have ever been, yet, at the same time, has embraced globalization with, arguably, much greater enthusiasm than was done, in earlier times, by Japan, Taiwan or Korea. If China's experience, however, is compared with that of other, more or less successful, developing countries, the similarities with the East Asia development model would seem to dwarf such differences

  12. OH reactivity in urban and suburban regions in Seoul, South Korea - an East Asian megacity in a rapid transition.

    Science.gov (United States)

    Kim, Saewung; Sanchez, Dianne; Wang, Mark; Seco, Roger; Jeong, Daun; Hughes, Stacey; Barletta, Barbara; Blake, Donald R; Jung, Jinsang; Kim, Deugsoo; Lee, Gangwoong; Lee, Meehye; Ahn, Joonyoung; Lee, Sang-Deok; Cho, Gangnam; Sung, Min-Young; Lee, Yong-Hwan; Kim, Dan Bi; Kim, Younha; Woo, Jung-Hun; Jo, Duseong; Park, Rokjin; Park, Jeong-Hoo; Hong, You-Deog; Hong, Ji-Hyung

    2016-07-18

    South Korea has recently achieved developed country status with the second largest megacity in the world, the Seoul Metropolitan Area (SMA). This study provides insights into future changes in air quality for rapidly emerging megacities in the East Asian region. We present total OH reactivity observations in the SMA conducted at an urban Seoul site (May-June, 2015) and a suburban forest site (Sep, 2015). The total OH reactivity in an urban site during the daytime was observed at similar levels (∼15 s(-1)) to those previously reported from other East Asian megacity studies. Trace gas observations indicate that OH reactivity is largely accounted for by NOX (∼50%) followed by volatile organic compounds (VOCs) (∼35%). Isoprene accounts for a substantial fraction of OH reactivity among the comprehensive VOC observational dataset (25-47%). In general, observed total OH reactivity can be accounted for by the observed trace gas dataset. However, observed total OH reactivity in the suburban forest area cannot be largely accounted for (∼70%) by the trace gas measurements. The importance of biogenic VOC (BVOCs) emissions and oxidations used to evaluate the impacts of East Asian megacity outflows for the regional air quality and climate contexts are highlighted in this study.

  13. Association between voluntary/involuntary job loss and the development of stroke or cardiovascular disease: a prospective study of middle-aged to older workers in a rapidly developing Asian country.

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    Mo-Yeol Kang

    Full Text Available The aim of this research was to investigate the association between job loss and the development of stroke or cardiovascular disease among middle-aged to older individuals in Korea. We also examined how this relationship was modified by gender and the nature of the job loss.This study used samples from the first- to fourth-wave datasets from the Korean Longitudinal Study of Aging (KLoSA, which were collected in 2006, 2008, 2010, and 2012. The study collected data from a total of 10,254 subjects aged ≥ 45 years at baseline. After applying exclusion criteria, the final sample size for analysis consisted of 4,000 individuals. Information about employment status, development of stroke or cardiovascular disease, and covariates (age, income level, and behavioral factors was obtained. Cox proportional hazards models were used to evaluate the association between voluntary/involuntary job loss and the development of stroke or cardiovascular disease. We performed these analyses separately according to disease, gender, and the nature of the job loss.Involuntary job loss significantly increased the risk of stroke or cardiovascular disease among males (adjusted hazard ratio [HR]  = 3.560, 95% confidence interval [CI]  = 2.055-6.168. Voluntary retirement also increased the risk of cardiovascular disease or stroke among males (adjusted HR = 2.879, 95% CI = 1.533-5.409. Job loss was more closely associated with stroke than with cardiovascular disease (stroke, adjusted HR = 6.208, 95% CI = 2.417-15.943; cardiovascular disease, adjusted HR = 2.768, 95% CI = 1.402-5.465.Our findings suggest that both voluntary retirement and involuntary job loss increase the risk for stroke or cardiovascular disease in middle-aged to older individuals, especially males.

  14. Design and Development of the European American Values Scale for Asian Americans.

    Science.gov (United States)

    Wolfe, Maren M.; Yang, Peggy H.; Wong, Eunice C.; Atkinson, Donald R.

    2001-01-01

    Introduces development of a scale that can be used along with the Asian Values Scale, to assess adherence to Asian cultural values. Used in combination these scales can measure Asian American acculturation to European American values. They make it possible to obtain a value and behavior assessment of Asian American acculturation. (JDM)

  15. Southeast Asian Languages - High Priority Materials Development Needs. Working Paper.

    Science.gov (United States)

    Jones, R. B.

    The material development needs for the Southeast Asian languages are analyzed as follows: (1) both student and reference grammars must be produced; (2) student and reference English-Foreign Language dictionaries are needed; (3) research is needed in sociolinguistics, semantic analyses, linguistic surveys; (4) elementary, intermediate and advanced…

  16. Rapidly Developing Toxic Epidermal Necrolysis

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    Viktoria Oline Barrios Poulsen

    2013-01-01

    Full Text Available Severe cutaneous reactions with potentially fatal outcomes can have many different causes. The Stevens-Johnson syndrome (SJS and toxic epidermal necrolysis (TEN are rare. They are characterized by a low incidence but high mortality, and drugs are most commonly implicated. Urgent active therapy is required. Prompt recognition and withdrawal of suspect drug and rapid intervention can result in favourable outcome. No further international guidelines for treatment exist, and much of the treatment relies on old or experimental concepts with no scientific evidence. We report on a 54-year-old man experiencing rapidly developing drug-induced severe TEN and presented multiorgan failure involving the respiratory and circulatory system, coagulopathy, and renal insufficiency. Detachment counted 30% of total body surface area (TBSA. SCORTEN = 5, indicating a mortality rate >90%. The patient was sedated and mechanically ventilated, supported with fluids and inotropes to maintain a stable circulation. Component therapy was guided by thromboelastography (TEG. The patient received plasmapheresis, and shock reversal treatment was initiated. He was transferred to a specialized intensive care burn unit within 24 hours from admittance. The initial care was continued, and hemodialysis was started. Pulmonary, circulatory, and renal sequelae resolved with intensive care, and re-epithelialization progressed slowly. The patient was discharged home on hospital day 19.

  17. South Asian Network for Development and Environmental ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    SANDEE) seeks to build research capacity in the area of poverty, economic development and environmental change in Bangladesh, Bhutan, India, Nepal, Pakistan, the Maldives and Sri Lanka. It does so through research, training, policy dialogue, ...

  18. South Asian Network for Development and Environmental ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    SANDEE) is a regional network that seeks to strengthen research capacity in the area of poverty, economic development and environmental change in seven countries (Bangladesh, Bhutan, India, Nepal, Pakistan, Maldives and Sri Lanka). It does so ...

  19. The Asian Development Model and Mining Reforms in Indonesia

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    John McLaren

    2015-07-01

    Full Text Available The aim of this paper is to provide accounting, marketing, management, finance and legal professionals who are engaged with emerging economies with an introduction to the ‘Asian Development Model’ and to use the mining reforms in Indonesia as an example of the Model in operation. This will assist those professionals in recognising the challenges faced by businesses in Australia and New Zealand when governments in South East Asian countries attempt to ‘catch up’ to the developed world and at the same time attempt to spread the benefits of the development to their people. The paper argues that there is an Asian Development Model and that the Indonesian mining reforms, in particular the requirement over time for 51 percent Indonesian Government ownership and the ban on the export of unprocessed resources, represent an attempt by the Indonesian State to speed up industrialisation in their country and to spread more of the benefits from mining to ordinary citizens in the recently democratised and politically decentralised country. In attempting to show strength however, the Indonesian state is exposing some weakness. The impact on jobs, revenue and production has been adverse although Foreign Direct Investment has increased. This latter may be because it is foreign multinational mining companies who are better placed than local mining enterprises to build smelters. The success of developing mining might be at the expense of local capital. In other words state intervention does not always produce all of the desired outcomes. It is not a panacea.

  20. Asian and African Development Trajectories Revisiting Facts and Figures

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    Gilles Carbonnier

    2013-05-01

    Full Text Available In today’s dominant discourse, the development trajectory of many East Asian countries is pictured as a success, whereas that of many sub-Saharan African countries is considered a failure. The Asian success stories often refer to the developmental state model, which highlights the pivotal role played by Asian political elites in catalyzing economic growth and broad-based development. The model includes economic liberalization and outward-oriented policies, with targeted support to – and protection of – strategic sectors and infant industries.How far is this underlying assumption supported by empirical evidence? This working paper examines a wide range of economic, social, institutional and governance indicators for a sample of six sub-Saharan and five South East Asian countries. Contrary to our research hypothesis, we did not find any significant difference in the level of government involvement in the domestic economy between the countries of the two regions, nor in the quality of institutions and governance indicators, nor in the share of imports and exports in GDP.Even if there are important gaps between the two regions, for instance with regard to the demographic transition, the agricultural sector or tertiary education, the picture is much more nuanced than portrayed by the dominant discourse. Our review of economic and socio-political indicators tells a rather different story, but not the whole story. Nevertheless, the indicators fail to take into account all the historical, institutional and structural factors that matter a great deal for development. Hence our analysis should be complemented by detailed country case studies to uncover the specific dynamics underlying different development trajectories and outcomes.

  1. The development and psychometric testing of East Asian Acculturation Scale among Asian immigrant women in Taiwan.

    Science.gov (United States)

    Kuo, Shu-Fen; Chang, Wen-Yin; Chang, Lu-I; Chou, Yu-Hua; Chen, Ching-Min

    2013-01-01

    This is a report of development and psychometric testing of the East Asian Acculturation Measure-Chinese version (EAAM-C) scale. An instrument validation design with a cross-sectional survey was conducted. The process was carried in two phases. In Phase 1, Barry's East Asian Acculturation Measure was translated and back translated to evaluate its content, face validity, and feasibility validity. In Phase 2, the 16-item EAAM-C was pilot-tested among 485 female immigrants for test-retest reliability, internal consistency, theoretically-supported construct validity and concurrent validity. The pilot work and the survey results indicated the tools possessed adequate content and face validity. The Cronbach's Alphas for the EAAM-C was 0.72, and 0.76-0.79 for its subscales, and the correlation of test-retest reliability (at 3 weeks) was 0.75. After dropping one item, four theoretically-supported factors which explained 61.82% of the variance were abstracted using exploratory factor analysis: assimilation, integration, separation, and marginalization. Based on the underlying four-factor theoretical structures of the EAAM, the confirmatory factor analysis of the EAAM-C was further examined. The analysis revealed that the four-factor model was an acceptable fit for the data which demonstrated adequate finding in its construct validity. These factors were inter-correlated, and showed statistically significant correlation with the Chinese Health Questionnaire, indicating adequate concurrent validity. The scale shows acceptable validity and consistency, and suggests that immigrant acculturation is a complex construct. This quick evaluation instrument can be applied to assess clients' acculturation and in further developing certain interventions to improve their health.

  2. High resolution melting analytical platform for rapid prenatal and postnatal diagnosis of β-thalassemia common among Southeast Asian population.

    Science.gov (United States)

    Prajantasen, Thanet; Fucharoen, Supan; Fucharoen, Goonnapa

    2015-02-20

    High resolution melting (HRM) analysis is a powerful technology for scanning sequence alteration. We have applied this HRM assay to screen common β-thalassemia mutations found among Southeast Asian population. Known DNA samples with 8 common mutations were used in initial development of the methods including -28 A-G, codon 17 A-T, IVSI-1G-T, IVSI-5G-C, codon 26G-A (Hb E), codons 41/42 -TTCT, codons 71/72+A and IVSII-654 C-T. Further validation was done on 60 postnatal and 6 prenatal diagnoses of β-thalassemia. Each mutation has specific HRM profile which could be used in rapid screening. Apart from those with DNA deletions, the results of HRM assay matched 100% with those of routine diagnosis made by routine allele specific PCR. In addition, the HRM assay could initially recognize three unknown mutations including a hitherto un-described one in Thai population. The established HRM assay should prove useful for rapid and high throughput platform for screening and prenatal diagnosis of β-thalassemia common in the region. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Developing Common Competencies for Southeast Asian General Dental Practitioners.

    Science.gov (United States)

    Chuenjitwongsa, Supachai; Poolthong, Suchit; Bullock, Alison; Oliver, Richard G

    2017-09-01

    Current policy in Southeast Asian dental education focuses on high-quality dental services from new dental graduates and the free movement of dental practitioners across the region. The Southeast Asian Nations (ASEAN) Dental Councils have proposed the "Common Major Competencies for ASEAN General Dental Practitioners" to harmonize undergraduate dental education. This article discusses how the ASEAN competencies were developed and established to assist the development of general dental practitioners with comparable knowledge, skills, and attitudes across ASEAN. The competencies were developed through four processes: a questionnaire about current national oral health problems, a two-round Delphi process that sought agreement on competencies, a panel discussion by representatives from ASEAN Dental Councils, and data verification by the representatives after the meeting. Key themes of the ASEAN competencies were compared with the competencies from the U.S., Canada, Europe, Australia, and Japan. A total of 33 competency statements, consistent with other regions, were agreed upon and approved. Factors influencing the ASEAN competencies and their implementation include oral health problems in ASEAN, new knowledge and technology in dentistry, limited institutional resources, underregulated dental schools, and uneven distribution of dental practitioners. The ASEAN competencies will serve as the foundation for further developments in ASEAN dental education including policy development, curriculum revision, quality assurance, and staff development. Collaboration amongst stakeholders is essential for successful harmonization of ASEAN dental education.

  4. Droughts in Asian Least Developed Countries: Vulnerability and sustainability

    Directory of Open Access Journals (Sweden)

    M. Alimullah Miyan

    2015-03-01

    Full Text Available Droughts occur both in developed and developing countries with significant impacts and are exacerbating in frequency, severity and duration. Over exploitation of water resources, weather variability and climate change are mostly responsible for such exacerbation. The impacts of droughts encompass the global ecosystem as a whole but vary from region to region. Least developed countries (LDCs are becoming the worst sufferer of the impacts due to physical, social and economic as well as knowledge and skills differences. The increasing biophysical vulnerability contexts and intensity in the Asian LDCs causing adverse effects on food security, human health, biodiversity, water resources, hydroelectric power generation, streams, perennial springs, and livelihood. Drought is also responsible for increasing pollution, pests and diseases and forced migration and famine. Information indicates monsoon has become erratic contributing to up-scaling of droughts. South and Southeast Asian LDCs like Bangladesh, Nepal, Bhutan, Cambodia and Lao PDR under the monsoon climatic zone have also been suffering from increasing droughts arising out of delayed and changing distribution patterns of precipitation. Prolong dry spells increase the frequencies of wildfire in grasslands, forests, and range-lands. The rain-fed crops of the plains are facing challenges from soil-moisture stress with projected droughts. Droughts causing migration of fishes, and marine anadromus species are having adverse impacts on spawning habitats. Reduction in annual surface runoff is decreasing the ground and surface water with negative effect on agriculture and water supply for industrial and domestic sectors. As droughts are exacerbating the consequences are accelerating. However, traditionally people are adapting with the changing situations applying indigenous knowledge and practices for sustainable living. This paper reflects on prevalence and impacts of droughts, existing coping

  5. WIST: toolkit for rapid, customized LIMS development

    National Research Council Canada - National Science Library

    Huang, Y Wayne; Arkin, Adam P; Chandonia, John-Marc

    2011-01-01

    Workflow Information Storage Toolkit (WIST) is a set of application programming interfaces and web applications that allow for the rapid development of customized laboratory information management systems (LIMS...

  6. Industrial pollution prevention programs in selected developing Asian countries

    Energy Technology Data Exchange (ETDEWEB)

    Chiu, Shen-yann [Argonne National Lab., IL (United States)]|[East-West Center, Honolulu, HI (United States)

    1995-12-31

    This paper presents the information on current activities to promote industrial pollution prevention (P2) in five selected Asian economies including Hong Kong, Republic of Korea, the Philippines, ROC in Taiwan, and Thailand. These activities, generally initiated in the last 5 years, are classified into 6 categories: awareness promotion, education and training, information transfer, technology development an demonstration, technical assistance, and financial incentives. Although participation is voluntary, these programs are all important at the early stages of P2 promotion and should be useful in informing industries of the benefit of P2 and helping them identify specific P2 measures as viable environmental management alternatives.

  7. Pandemic influenza preparedness and health systems challenges in Asia: results from rapid analyses in 6 Asian countries

    Directory of Open Access Journals (Sweden)

    Putthasri Weerasak

    2010-06-01

    Full Text Available Abstract Background Since 2003, Asia-Pacific, particularly Southeast Asia, has received substantial attention because of the anticipation that it could be the epicentre of the next pandemic. There has been active investment but earlier review of pandemic preparedness plans in the region reveals that the translation of these strategic plans into operational plans is still lacking in some countries particularly those with low resources. The objective of this study is to understand the pandemic preparedness programmes, the health systems context, and challenges and constraints specific to the six Asian countries namely Cambodia, Indonesia, Lao PDR, Taiwan, Thailand, and Viet Nam in the prepandemic phase before the start of H1N1/2009. Methods The study relied on the Systemic Rapid Assessment (SYSRA toolkit, which evaluates priority disease programmes by taking into account the programmes, the general health system, and the wider socio-cultural and political context. The components under review were: external context; stewardship and organisational arrangements; financing, resource generation and allocation; healthcare provision; and information systems. Qualitative and quantitative data were collected in the second half of 2008 based on a review of published data and interviews with key informants, exploring past and current patterns of health programme and pandemic response. Results The study shows that health systems in the six countries varied in regard to the epidemiological context, health care financing, and health service provision patterns. For pandemic preparation, all six countries have developed national governance on pandemic preparedness as well as national pandemic influenza preparedness plans and Avian and Human Influenza (AHI response plans. However, the governance arrangements and the nature of the plans differed. In the five developing countries, the focus was on surveillance and rapid containment of poultry related transmission

  8. An overview of BIM uptake in Asian developing countries

    Science.gov (United States)

    Ismail, Noor Akmal Adillah; Chiozzi, Maria; Drogemuller, Robin

    2017-11-01

    BIM is increasingly in demand within the construction industry internationally in recent years. The application of the technology reconciles several problems within the project teams such as delays, rework, miscommunication, and other related to inefficiencies that affect project success. While it is actively employed by the majority of the developed countries, however, BIM is not as advanced in most developing countries. Therefore, this paper reviews BIM uptake in some of the Asian developing countries and examines the extent to which it is implemented in these regions. Prevalent challenges were considered with recommendations towards addressing the issues of low level of BIM adoption that distinguishes the developing from the developed countries. This paper will provide some insights of how BIM is evolving within those countries considering the drivers and barriers in adopting the technology and how this is likely to change in the near future.

  9. The East Asian Regional Office of Astronomy for Development

    Science.gov (United States)

    de Grijs, Richard; Zhang, Ziping; He, Jinhua

    2016-10-01

    At the 2012 General Assembly of the International Astronomical Union (IAU), the Office of Astronomy for Development announced a number of exciting new partnerships to assist with the IAU's decadal strategic plan (2010-2020). These landmark decisions included establishing a new coordinating centre that aims at using astronomy as a tool for development in East Asia. The agreement covers two important functions. One is known as a Regional Node, which entails the coordination of astronomy-for-development activities in countries within the general geographical region of East Asia. The other is known as a Language Expertise Centre which deals with all aspects relating to (mainly) the Chinese language and culture. The impact of the latter may obviously spread well beyond the geographical region to other parts of the world. Here we provide an update of the achievements and aims of the East Asian Office of Astronomy for Development.

  10. Energy Development and Environmental NGOs: The Asian Perspective

    Energy Technology Data Exchange (ETDEWEB)

    Gan, Lin

    1998-12-01

    This report compares and analyses the energy-sector activities of non-governmental organizations (NGOs) in Asia. The main focus is on energy development with an environmental orientation such as large hydroelectric power development, energy efficiency improvements, and new and renewable energy development. Specifically, it (1) analyses the motivations, aims, characteristics and means of operation of the NGOs in their involvement in projects related to energy and environment, (2) studies the process of NGOs involvement in decision-making and the extent to which processes are affected by the nature of operation and why, (3) reviews the relationships and means of interaction of NGOs with governmental agencies, international aid organizations and the private sector, (4) examines the influence of international NGOs on local and regional NGOs and the differences in their values and modes of operation, and finally (5) describes the implications of NGO contributions to the emergence of global civil society from an Asian perspective. 28 refs., 1 fig., 1 tab.

  11. Practical implications of rapid development methodologies

    CSIR Research Space (South Africa)

    Gerber, A

    2007-11-01

    Full Text Available Rapid development methodologies are popular approaches for the development of modern software systems. The goals of these methodologies are the inclusion of the client into the analysis, design and implementation activities, as well...

  12. Development of a Rapid Thermoplastic Impregnation Device

    NARCIS (Netherlands)

    Weustink, A.P.D.

    2007-01-01

    A melt impregnation device for rapid thermoplastic impregnation of fiber bundles has been developed through modeling and experiments. The basic principles behind the thermoplastic impregnation process are investigated and the properties needed for a successful thermoplastic impregnation device are

  13. Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus).

    Science.gov (United States)

    Paudel, Sarad; Villanueva, Marvin A; Mikota, Susan K; Nakajima, Chie; Gairhe, Kamal P; Subedi, Suraj; Rayamajhi, Nabin; Sashika, Mariko; Shimozuru, Michito; Matsuba, Takashi; Suzuki, Yasuhiko; Tsubota, Toshio

    2016-08-01

    We developed an interferon-γ release assay (IGRA) specific for Asian elephants (Elephas maximus). Whole blood collected from forty captive Asian elephants was stimulated with three different mitogens i.e., phytohemagglutinin (PHA), pokweed mitogen (PWM) and phorbol myristate aceteate/ionomycin (PMA/I). A sandwich ELISA that was able to recognize the recombinant elephant interferon-γ (rEIFN-γ) as well as native interferon-γ from the Asian elephants was performed using anti-elephant IFN-γ rabbit polyclonal antibodies as capture antibodies and biotinylated anti-elephant IFN-γ rabbit polyclonal antibodies as detection antibodies. PMA/I was the best mitogen to use as a positive control for an Asian elephant IGRA. The development of an Asian elephant-specific IGRA that detects native IFN-γ in elephant whole blood provides promising results for its application as a potential diagnostic tool for diseases, such as tuberculosis (TB) in Asian elephants.

  14. The East Asian Office of Astronomy for Development

    Science.gov (United States)

    de Grijs, Richard; Zhang, Ziping

    2015-08-01

    At the 2012 General Assembly of the International Astronomical Union (IAU), the Office of Astronomy for Development (OAD) programme announced a number of exciting new partnerships to assist with the IAU's decadal strategic plan (2010-2020). These landmark decisions included establishing a new coordinating centre that aims at using astronomy as a tool for development in East Asia. The agreement covers two important functions. One is known as a Regional Node, which entails the coordination of astronomy-for-development activities in countries within the general geographical region of East Asia (in first instance China, Mongolia and the DPRK, but without placing firm geographical limits on the region). The other is known as a Language Expertise Centre which will deal with all aspects relating to (mainly) the Chinese language and culture. The impact of the latter may obviously spread well beyond the geographical region to other parts of the world. At this next General Assembly, we aim at updating the community of the achievements and aims of the East Asian Office of Astronomy for Development.

  15. Carboxylesterase 1A2 encoding gene with increased transcription and potential rapid drug metabolism in Asian populations

    DEFF Research Database (Denmark)

    Rasmussen, Henrik Berg; Madsen, Majbritt Busk; Lyauk, Yassine Kamal

    2017-01-01

    The carboxylesterase 1 gene (CES1) encodes a hydrolase implicated in the metabolism of commonly used drugs. CES1A2, a hybrid of CES1 and a CES1-like pseudogene, has a promoter that is weak in most individuals. However, some individuals harbor a promoter haplotype of this gene with two overlapping...... Sp1 sites that confer significantly increased transcription potentially leading to rapid drug metabolism. This CES1A2 haplotype has previously been reported to be common among Asians. Using polymerase chain reaction followed by sequencing, the present study examined variation in the promoter and 5...

  16. New developments in rapidly solidified magnesium alloys

    Energy Technology Data Exchange (ETDEWEB)

    Das, S.K. [Allied-Signal, Inc., Morristown, NJ (United States); Chang, C.F. [Allied-Signal, Inc., Morristown, NJ (United States); Raybould, D. [Allied-Signal, Inc., Morristown, NJ (United States); King, J.F. [Magnesium Elektron Ltd., Manchester (United Kingdom); Thistlethwaite, S. [Magnesium Elektron Ltd., Manchester (United Kingdom)

    1992-12-31

    In the present paper, we will examine the new developments in the rapidly solidified Mg-Al-Zn-Nd (EA55RS) alloy. We shall first briefly review the process scale-up currently employed for producing rapidly solidified magnesium alloys in large quantities, and then discuss the effect of billet size and processing parameters on the mechanical properties of various mill product forms such as extrusions and sheets. The superplastic behavior of EA55RS extrusions and rolled sheets are also discussed. Finally, some results on magnesium metal-matrix composites using rapidly solidified EA55RS matrix powders and SiC particulates are presented. (orig.)

  17. Infant feeding effects on early neurocognitive development in Asian children.

    Science.gov (United States)

    Cai, Shirong; Pang, Wei Wei; Low, Yen Ling; Sim, Lit Wee; Sam, Suet Chian; Bruntraeger, Michaela Bianka; Wong, Eric Qinlong; Fok, Doris; Broekman, Birit F P; Singh, Leher; Richmond, Jenny; Agarwal, Pratibha; Qiu, Anqi; Saw, Seang Mei; Yap, Fabian; Godfrey, Keith M; Gluckman, Peter D; Chong, Yap-Seng; Meaney, Michael J; Kramer, Michael S; Rifkin-Graboi, Anne

    2015-02-01

    Breastfeeding has been shown to enhance global measures of intelligence in children. However, few studies have examined associations between breastfeeding and specific cognitive task performance in the first 2 y of life, particularly in an Asian population. We assessed associations between early infant feeding and detailed measures of cognitive development in the first 2 y of life in healthy Asian children born at term. In a prospective cohort study, neurocognitive testing was performed in 408 healthy children (aged 6, 18, and 24 mo) from uncomplicated pregnancies (i.e., birth weight >2500 and <4000 g, gestational age ≥37 wk, and 5-min Apgar score ≥9). Tests included memory (deferred imitation, relational binding, habituation) and attention tasks (visual expectation, auditory oddball) as well as the Bayley Scales of Infant and Toddler Development, Third Edition (BSID-III). Children were stratified into 3 groups (low, intermediate, and high) on the basis of breastfeeding duration and exclusivity. After potential confounding variables were controlled for, significant associations and dose-response relations were observed for 4 of the 15 tests. Higher breastfeeding exposure was associated with better memory at 6 mo, demonstrated by greater preferential looking toward correctly matched items during early portions of a relational memory task (i.e., relational binding task: P-trend = 0.015 and 0.050 for the first two 1000-ms time bins, respectively). No effects of breastfeeding were observed at 18 mo. At 24 mo, breastfed children were more likely to display sequential memory during a deferred imitation memory task (P-trend = 0.048), and toddlers with more exposure to breastfeeding scored higher in receptive language [+0.93 (0.23, 1.63) and +1.08 (0.10, 2.07) for intermediate- and high-breastfeeding groups, respectively, compared with the low-breastfeeding group], as well as expressive language [+0.58 (-0.06, 1.23) and +1.22 (0.32, 2.12) for intermediate- and high

  18. The Development of White-Asian Categorization : Contributions from Skin Color and Other Physiognomic Cues

    NARCIS (Netherlands)

    Dunham, Yarrow; Dotsch, Ron|info:eu-repo/dai/nl/328554197; Clark, Amelia R; Stepanova, Elena V

    2016-01-01

    We examined the development of racial categorizations of faces spanning the European-East Asian ("White-Asian") categorical continuum in children between the ages of four and nine as well as adults. We employed a stimulus set that independently varied skin color and other aspects of facial

  19. The development of White-Asian categorization: Contributions from skin color and other physiognomic cues

    NARCIS (Netherlands)

    Dunham, Y.; Dotsch, R.; Clark, A.R.; Stepanova, E.V.

    2016-01-01

    We examined the development of racial categorizations of faces spanning the European-East Asian ("White-Asian") categorical continuum in children between the ages of four and nine as well as adults. We employed a stimulus set that independently varied skin color and other aspects of facial

  20. The Asian American Racism-Related Stress Inventory: Development, Factor Analysis, Reliability, and Validity

    Science.gov (United States)

    Liang, Christopher T. H.; Li,Lisa C.; Kim, Bryan S. K.

    2004-01-01

    The development of the 29-item Asian American Racism-Related Stress Inventory (AARRSI) is presented. In the first study, data from 161 Asian American respondents were subjected to an exploratory factor analysis, which yielded 3 subscales composed of Socio-Historical Racism (14 items), General Racism (8 items), and Perpetual Foreigner Racism (7…

  1. Development and tendency of rapid prototyping technology

    Science.gov (United States)

    Yan, Yongnian; Hong, Guodong

    1998-08-01

    The definition of the rapid prototyping is given in this paper. Various RP processes, which build the prototypes with 2.5 or 3 dimensional layers, are introduced. The relative techniques of RP and the differences between RP technique and CNC manufacturing are analyzed. The paper discusses the RP's applied fields and methods and presents the RP development in the world. According to the idea that requirements determine the developing, the RP's tendency is discussed.

  2. Strategies and models for agricultural sustainability in developing Asian countries.

    Science.gov (United States)

    Kesavan, P C; Swaminathan, M S

    2008-02-27

    The green revolution of the 1960s and 1970s which resulted in dramatic yield increases in the developing Asian countries is now showing signs of fatigue in productivity gains. Intensive agriculture practiced without adherence to the scientific principles and ecological aspects has led to loss of soil health, and depletion of freshwater resources and agrobiodiversity. With progressive diversion of arable land for non-agricultural purposes, the challenge of feeding the growing population without, at the same time, annexing more forestland and depleting the rest of life is indeed daunting. Further, even with food availability through production/procurement, millions of marginal farming, fishing and landless rural families have very low or no access to food due to lack of income-generating livelihoods. Approximately 200 million rural women, children and men in India alone fall in this category. Under these circumstances, the evergreen revolution (pro-nature, pro-poor, pro-women and pro-employment/livelihood oriented ecoagriculture) under varied terms are proposed for achieving productivity in perpetuity. In the proposed 'biovillage paradigm', eco-friendly agriculture is promoted along with on- and non-farm eco-enterprises based on sustainable management of natural resources. Concurrently, the modern ICT-based village knowledge centres provide time- and locale-specific, demand-driven information needed for evergreen revolution and ecotechnologies. With a system of 'farm and marine production by masses', the twin goals of ecoagriculture and eco-livelihoods are addressed. The principles, strategies and models of these are briefly discussed in this paper.

  3. Traps as treats: a traditional sticky rice snack persisting in rapidly changing Asian kitchens.

    Science.gov (United States)

    Schwallier, Rachel; de Boer, Hugo J; Visser, Natasja; van Vugt, Rogier R; Gravendeel, Barbara

    2015-03-24

    An accessory to modern developing economies includes a shift from traditional, laborious lifestyles and cuisine to more sedentary careers, recreation and convenience-based foodstuffs. Similar changes in the developed western world have led to harmful health consequences. Minimization of this effect in current transitional cultures could be met by placing value on the maintenance of heritage-rich food. Vitally important to this is the preservation and dissemination of knowledge of these traditional foods. Here, we investigate the history and functionality of a traditional rice snack cooked in Nepenthes pitchers, one of the most iconic and recognizable plants in the rapidly growing economic environment of Southeast Asia. Social media was combined with traditional ethnobotanical surveys to conduct investigations throughout Malaysian Borneo. Interviews were conducted with 25 market customers, vendors and participants from various ethnical groups with an in-depth knowledge of glutinous rice cooked in pitcher plants. The acidity of pitcher fluid was measured during experimental cooking to analyze possible chemical avenues that might contribute to rice stickiness. Participants identifying the snack were almost all (96%) from indigenous Bidayuh or Kadazandusun tribal decent. They prepare glutinous rice inside pitcher traps for tradition, vessel functionality and because they thought it added fragrance and taste to the rice. The pH and chemical activity of traps analyzed suggest there is no corresponding effect on rice consistency. Harvest of pitchers does not appear to decrease the number of plants in local populations. The tradition of cooking glutinous rice snacks in pitcher plants, or peruik kera in Malay, likely carries from a time when cooking vessels were more limited, and persists only faintly in tribal culture today because of value placed on maintaining cultural heritage. Social media proved a valuable tool in our research for locating research areas and in

  4. YAM- A Framework for Rapid Software Development

    Science.gov (United States)

    Jain, Abhinandan; Biesiadecki, Jeffrey

    2006-01-01

    YAM is a software development framework with tools for facilitating the rapid development and integration of software in a concurrent software development environment. YAM provides solutions for thorny development challenges associated with software reuse, managing multiple software configurations, the development of software product-lines, multiple platform development and build management. YAM uses release-early, release-often development cycles to allow developers to incrementally integrate their changes into the system on a continual basis. YAM facilitates the creation and merging of branches to support the isolated development of immature software to avoid impacting the stability of the development effort. YAM uses modules and packages to organize and share software across multiple software products. It uses the concepts of link and work modules to reduce sandbox setup times even when the code-base is large. One side-benefit is the enforcement of a strong module-level encapsulation of a module s functionality and interface. This increases design transparency, system stability as well as software reuse. YAM is in use by several mid-size software development teams including ones developing mission-critical software.

  5. South Asian co-operation in population education. Materials jointly developed for common use by South Asian population education programmes.

    Science.gov (United States)

    1992-01-01

    Southern Asian population education programs have developed common materials on population and family life education. Countries involved were Bangladesh, India, Nepal, Pakistan, and Sri Lanka. The development of materials occurred as a byproduct of workshop conducted in Nepal from December 3-7, 1990 and December 2-10, 1991 in Sri Lanka. The 1st meeting was organized by UNESCO's Population Education Advisory team, and 6 curriculum topics were identified. Pretesting of materials was conducted between meetings. The final product was a set of 10 posters and 2 comic strips on the quality of life developed by India for elementary level use; a family life and sex education syllabus developed by Sri Lanka for secondary school use; 5 modules with teacher's guides and sample lessons for secondary school use; 5 modules and a teacher's guide on transmission of values on population education by Pakistan; 25 flip charts on maternal and child health for illiterates developed by Nepal; and a field guide on environmental protection for nonformal field workers developed by Bangladesh. Materials were designed through brainstorming sessions, designing of materials by experts, review by other groups, and retesting on target audiences. Revision followed pretesting. The plan for assuring use of materials was to have UNESCO print prototypes and then participants would seek financial support for country supplies. A suggestion was made to leave ample space for insertion of local language captions. Another suggestion was that the cartoon strip "Girls are Pearls" be printed on students' exercise books for all member countries. Member countries should also have available selected materials translated into English and distributed. UNESCO should continue to play the role of facilitator of information and expertise exchange among member countries. Another mutually cooperative activity was the Group Training Course on Population Education for the South Asian subregion held in December 1991.

  6. Rapid Glass Refiner Development Program, Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-20

    A rapid glass refiner (RGR) technology which could be applied to both conventional and advanced class melting systems would significantly enhance the productivity and the competitiveness of the glass industry in the United States. Therefore, Vortec Corporation, with the support of the US Department of Energy (US DOE) under Cooperative Agreement No. DE-FC07-90ID12911, conducted a research and development program for a unique and innovative approach to rapid glass refining. To provide focus for this research effort, container glass was the primary target from among the principal glass types based on its market size and potential for significant energy savings. Container glass products represent the largest segment of the total glass industry accounting for 60% of the tonnage produced and over 40% of the annual energy consumption of 232 trillion Btu/yr. Projections of energy consumption and the market penetration of advanced melting and fining into the container glass industry yield a potential energy savings of 7.9 trillion Btu/yr by the year 2020.

  7. The Development of White-Asian Categorization: Contributions from Skin Color and Other Physiognomic Cues.

    Science.gov (United States)

    Dunham, Yarrow; Dotsch, Ron; Clark, Amelia R; Stepanova, Elena V

    2016-01-01

    We examined the development of racial categorizations of faces spanning the European-East Asian ("White-Asian") categorical continuum in children between the ages of four and nine as well as adults. We employed a stimulus set that independently varied skin color and other aspects of facial physiognomy, allowing the contribution of each to be assessed independently and in interaction with each other. Results demonstrated substantial development across this age range in children's ability to draw on both sorts of cue, with over twice as much variance explained by stimulus variation in adults than children. Nonetheless, children were clearly sensitive to both skin color and other aspects of facial physiognomy, suggesting that understanding of the White-Asian category boundary develops in a somewhat different way than understanding of the White-Black category boundary, in which attention to features other than skin color appear only somewhat later. Discussion focuses on the implications of these findings for theories of social categorization.

  8. Cultural Tension and Career Development for Asian American College Students: A Phenomenological Study

    Science.gov (United States)

    Lee, Eddie Kyo

    2013-01-01

    This mixed methods, phenomenological study examined how cultural tension influences career development for Asian American community college students. Students initially completed Phinney's (1992) Multigroup Ethnic Identity Measure (MEIM) and an instrument developed for this study called the Cultural Identification Survey. The mean for ethnic…

  9. Dialysis and Quality of Dialysate in Southeast Asian Developing Countries

    Directory of Open Access Journals (Sweden)

    Tomotaka Naramura

    2014-04-01

    Full Text Available Background: The number of dialysis patients has been increasing in Southeast Asia, but statistical data about these patients and on the quality of dialysates in Southeast Asian dialysis facilities are still imprecise. For this study, dialysis-related statistical data were collected in Southeast Asia. Methods: A survey of the quality of dialysates was carried out at 4 dialysis facilities in Vietnam and Cambodia. The dialysis patient survey included the numbers of dialysis facilities and patients receiving dialysis, a ranking of underlying diseases causing the initiation of dialysis, the number of patients receiving hemodialysis (HD/on-line hemodiafiltration/continuous ambulatory peritoneal dialysis, the number of HD monitoring devices installed, the cost of each session of dialysis (in USD, the percentage of out-of-pocket payments, and the 1-year survival rates of the dialysis patients (in percent. The dialysate survey covered the endotoxin (ET level and bacterial count in tap water, in water filtered through a reverse osmosis system and in dialysate. Results: In each of the countries, the most frequent reason for the initiation of dialysis is diabetes mellitus. HD is usually carried out according to the ‘reuse' principle. The 1-year survival rates are 70% in Myanmar and about 90% in the Philippines and Malaysia. The ET levels in standard dialysates were satisfactory at 2 facilities. The bacterial counts in dialysates were not acceptable at any of the facilities investigated. Conclusion: There is an urgent need to teach medical workers involved in dialysis how to prepare sterile and ET-free dialysates.

  10. Effects of host wood moisture on the life cycle development of the Asian longhorned beetle

    Science.gov (United States)

    Alexandra N. Whitney; Melody A. Keena

    2011-01-01

    The Asian longhorned beetle, Anoplophora glabripennis, is an alien invasive species recently introduced to the United States in larval form within solid wood packing material from China. Larvae develop within the xylem of apparently healthy trees. In the U.S., the species has been found infesting 21 species of hardwoods, across 12 genera, with maple...

  11. Asian tigers, African lions : comparing the development performance of Southeast Asia and Africa

    NARCIS (Netherlands)

    Berendsen, B.; Dietz, A.J.; Schulte, Nordholt H.; Veen, van der R.

    2013-01-01

    Asian Tigers, African Lions is an anthology of contributions by scholars and (former) diplomats related to the 'Tracking Development' research project, funded by the Netherlands Ministry of Foreign Affairs, and coordinated by the African Studies Centre and KITLV, both in Leiden, in collaboration

  12. Soil contamination by brominated flame retardants in open waste dumping sites in Asian developing countries.

    Science.gov (United States)

    Eguchi, Akifimi; Isobe, Tomohiko; Ramu, Karri; Tue, Nguyen Minh; Sudaryanto, Agus; Devanathan, Gnanasekaran; Viet, Pham Hung; Tana, Rouch Seang; Takahashi, Shin; Subramanian, Annamalai; Tanabe, Shinsuke

    2013-03-01

    In Asian developing countries, large amounts of municipal wastes are dumped into open dumping sites each day without adequate management. This practice may cause several adverse environmental consequences and increase health risks to local communities. These dumping sites are contaminated with many chemicals including brominated flame retardants (BFRs) such as polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecanes (HBCDs). BFRs may be released into the environment through production processes and through the disposal of plastics and electronic wastes that contain them. The purpose of this study was to elucidate the status of BFR pollution in municipal waste dumping sites in Asian developing countries. Soil samples were collected from six open waste dumping sites and five reference sites in Cambodia, India, Indonesia, Malaysia, and Vietnam from 1999 to 2007. The results suggest that PBDEs are the dominant contaminants in the dumping sites in Asian developing countries, whereas HBCD contamination remains low. Concentrations of PBDEs and HBCDs ranged from ND to 180 μg/kg dry wt and ND to 1.4 μg/kg dry wt, respectively, in the reference sites and from 0.20 to 430 μg/kg dry wt and ND to 2.5 μg/kg dry wt, respectively, in the dumping sites. Contamination levels of PBDEs in Asian municipal dumping sites were comparable with those reported from electronic waste dismantling areas in Pearl River delta, China. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. WHITHER DEVELOPMENT? THE EFFECTS OF THE EURASIAN UNION ON THE CENTRAL ASIAN REPUBLICS

    Directory of Open Access Journals (Sweden)

    Adelin Dumitru

    2015-12-01

    Full Text Available In this paper I analyze the impact of the Eurasian Union on the Central Asian republics, with a focus on remittances trends. To this end I review at first the literature regarding the effects of the Customs Union on its members. Then, I assess the current state of the economies most likely to be affected by membership in the Eurasian Union, i.e. Tajikistan and Kyrgyzstan, whose specificity is their dependency on remittances. Secondly, I present a plausible scenario in regard to the influence of the formalization of this regional organization on the emerging Central Asian regional security subcomplex. I argue that the EEU is a hindrance towards the five Central Asian Republics’ evolving towards a regional security complex. Not only has it already distorted trade in the region, but it can also turn some presently frozen conflicts into security hotspots. I hold that the only way to spur development in the region is, internally, to diversify the economy, and, externally, to bring the five republics closer. I try to show that the Central Asian Republics should simultaneously pursue a switch from a balance of threat to regional integration and sustainable national development. Nonetheless, the EEU will have at best mixed effects when it comes to these necessities. Alternatively, China and the New Silk Road initiative that it endorses might contribute to de-securitizing some of the existing issues.

  14. Development of an Asian American parental racial-ethnic socialization scale.

    Science.gov (United States)

    Juang, Linda P; Shen, Yishan; Kim, Su Yeong; Wang, Yijie

    2016-07-01

    To develop a measure of parental racial-ethnic socialization that is appropriate for Asian American families. To test the reliability and validity of this new measure, we surveyed 575 Asian American emerging adults (49% female, 79% U.S. born). Using exploratory and confirmatory factor analyses, the results show 7 reliable subscales: maintenance of heritage culture, becoming American, awareness of discrimination, avoidance of other groups, minimization of race, promotion of equality, and cultural pluralism. Tests of factorial invariance show that overall, the subscales demonstrate, at minimum, partial metric invariance across gender, age, nativity, educational attainment, parent educational attainment, geographic region of residence, and Asian-heritage region. Thus, the relations among the subscales with other variables can be compared across these different subgroups. The subscales also correlated with ethnic identity, ethnic centrality, perceptions of discrimination, and pluralistic orientation, demonstrating construct validity. In an increasingly complex and diverse social world, our scale will be useful for gaining a better understanding of how Asian American parents socialize their children regarding issues of race, discrimination, culture, and diversity. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  15. Conflict between conservation and development: cash forest encroachment in Asian elephant distributions

    OpenAIRE

    Liu, Peng; Wen, Hui; Harich, Franziska K.; He, Changhuan; Wang, Lanxin; Guo, Xianming; Zhao, Jianwei; Luo, Aidong; Yang, Hongpei; Sun, Xiao; Yu, Yang; Zheng, Shaobo; Guo, Jing; Li, Li; Zhang, Li

    2017-01-01

    Over the last 4 decades, China has undergone major economic development, resulting in considerable impacts on its wildlife populations and habitats. It is essential to quantify the conflict between development and conservation to assist with policy-making because forestry policies and market trends affected indirectly the distribution of Asian elephants. Here, we mapped the historical distribution of elephants versus human land use. Elephant distributions appear to occur in unbroken natural f...

  16. Parenting and Children's Socioemotional and Academic Development among White, Latino, Asian, and Black families

    OpenAIRE

    Kang, Hannah Soo

    2014-01-01

    ABSTRACT OF THE DISSERTATIONParenting and Children's Socioemotional and Academic Development among White, Latino, Asian, and Black familiesByHannah S. KangDoctor of Philosophy in Psychology and Social BehaviorUniversity of California, Irvine 2014Professor Chuansheng Chen, ChairA large body of research has demonstrated the crucial role of parenting in children's socioemotional and academic development (Maccoby & Martin, 1983). This literature, however, has major limitations in the following th...

  17. The challenges of rapid urbanization on sustainable development of ...

    African Journals Online (AJOL)

    The study examines the challenges of rapid urbanization on the sustainable development of Nyanya, Abuja. An interesting finding of the study is that the primary factor for the rapid urbanization of Nyanya within a short period of time is migration. The consequence of this rapid urbanization and population rise within a short ...

  18. Transnational corporations from Asian developing countries: The internationalisation characteristics and business strategies of Sime Darby Berhad

    Directory of Open Access Journals (Sweden)

    Ahmad, S.Z.

    2008-01-01

    Full Text Available There is limited empirical research on the internationalisation processes, strategies and operations of Asian multinational corporations (MNCs, particularly MNC’s based in Malaysia. The emergence and development of an MNC from this developing country represents a significant addition to the literature on this topic which augments and supplements the information already available with regard to nascent MNCs from Asian Newly Industrialised Countries (NIC’s. Drawing on primary data from in-depth interviews with 12 key executives from Sime Darby Berhad (SDB, a developing Malaysian-based MNC, this paper will examine and investigate the firm’s internationalisation process, its characteristics and strategies, including motivations, patterns, and sources of competitive advantage. The empirical findings, limitations and areas for further research are discussed.

  19. The IAU's East Asian Regional Office of Astronomy for Development

    Science.gov (United States)

    de Grijs, Richard

    2014-09-01

    At the 2012 General Assembly of the International Astronomical Union (IAU), the Office of Astronomy for Development (OAD) programme announced a number of exciting new partnerships to assist with the IAU's decadal strategic plan (2010-2020). These landmark decisions included establishing a new coordinating centre that aims at using astronomy as a tool for development in East Asia. The agreement covers two important functions. One is known as a Regional Node, which entails the coordination of astronomy-for-development activities in countries within the general geographical region of East Asia (in first instance China, Mongolia and the DPRK, but without placing firm geographical limits on the region). The other is known as a Language Expertise Centre which will deal with all aspects relating to (mainly) the Chinese language and culture. The impact of the latter may obviously spread well beyond the geographical region to other parts of the world.

  20. The Contribution of East Asian Countries to Internationally Published Asian Higher Education Research: The Role of System Development and Internationalization

    Science.gov (United States)

    Jung, Jisun; Horta, Hugo

    2015-01-01

    Studies of higher education by scholars based in Asia have been growing in volume, following worldwide trends. To a large extent, this growth has been driven by East Asian countries, but little is known about the characteristics of the contribution of these countries. This study analyses their overall and specific contribution. The paper concludes…

  1. URBAN TRANSPORT AND LOCAL GOVERNANCE IN ASIAN DEVELOPING COUNTRIES

    Directory of Open Access Journals (Sweden)

    Akira MORITA

    2004-01-01

    This paper comprises a GIS-based land use analysis on the relationship between urbanization and transport infrastructure development, b GPS-based travel behavior survey, and c interview survey on residents' satisfaction with transport infrastructures and services. It was shown that the current land use patterns largely differ depending on the existence of agricultural infrastructure development in the pre-urbanized stage. It was also confirmed by a GPS-based travel survey that travel behavior patterns in scattered development areas are significantly different from those in orderly development areas. The former areas lack not only road space but also a structured hierarchy of networks, resulting in inefficient travel behaviors with low speed and detours. The GPS survey gave clearer pictures to grasp the relationship between travel patterns of residents and their demand for the improvement of local transport services. It was indicated that local governments who are responsible for these demands often fail to meet them due to financial and institutional limitations of the current system.

  2. [Rapid development of cosmetic medicine in China].

    Science.gov (United States)

    Lu, Kaihua; Pan, Baohua; Xia, Wei

    2006-04-01

    To review and summarize the development during the last 20 years and the current status of cosmetic medicine, i.e., cosmetic surgery, in China, for the healthier development of this specialty in the future. Literature concerned was reviewed, including conference abstracts, papers, and publications, and the present status and problems were analyzed. Cosmetic medicine was recognized as an independent specialty and gained its clear definition. The development of cosmetic medicine is an inevitable trend of the changing medical modules and the developing science and civilization. This trend fulfilled the need of the people. The related problems consisted of a high complication rate, confusion of management, and insufficient specific knowledge in part of the providers. The development of cosmetic medicine is an inevitable trend of the civilization development. For the healthy development of this specialty, scientific management and systemic education for the providers are crucial. Only those who have the plastic surgery background are able to participate in this practice.

  3. Heterochronies in the cranial development of Asian tree frogs (Amphibia: Anura: Rhacophoridae) with different life histories.

    Science.gov (United States)

    Vassilieva, A B

    2017-03-01

    The development of bony skull was studied in four species of Asian tree frogs (Rhacophoridae) with different life histories: biphasic development with free larval stage and direct development. In biphasic rhacophorids the sequence of the appearance of cranial bones generally followed the generalized pattern of craniogenesis, which was described for most studied anurans. In contrast, direct-developing species displayed some heterochronies in the formation of skull bones, namely, the accelerated formation of the anlagen of jaw and suspensorium bones. The obtained results support that the embryonization in amphibians is regularly accompanied by a heterochronic repatterning of craniogenesis, rather similar in different phyletic groups.

  4. Modular Infrastructure for Rapid Flight Software Development

    Science.gov (United States)

    Pires, Craig

    2010-01-01

    This slide presentation reviews the use of modular infrastructure to assist in the development of flight software. A feature of this program is the use of model based approach for application unique software. A review of two programs that this approach was use on are: the development of software for Hover Test Vehicle (HTV), and Lunar Atmosphere and Dust Environment Experiment (LADEE).

  5. Rapid Development of Intestinal Type Gastric Adenocarcinoma

    Directory of Open Access Journals (Sweden)

    Young S. Oh

    2011-04-01

    Full Text Available Intestinal type gastric adenocarcinoma is felt to develop over a protracted time period through a series of defined steps. Several potential risk factors for the development of gastric cancer have been identified, including a family history of gastric cancer and Helicobacter pylori infection. We present the case of a patient with neither risk factor who progressed in a 14 month time frame from histologically normal gastric mucosa to early stage intestinal type gastric adenocarcinoma in the setting of diffuse gastric intestinal metaplasia and atrophic gastritis. This patient’s presentation conflicts with our current understanding of the development of intestinal type gastric adenocarcinoma.

  6. Development and evaluation of the Internalized Racism in Asian Americans Scale (IRAAS).

    Science.gov (United States)

    Choi, Andrew Young; Israel, Tania; Maeda, Hotaka

    2017-01-01

    This article presents the development and psychometric evaluation of the Internalized Racism in Asian Americans Scale (IRAAS), which was designed to measure the degree to which Asian Americans internalized hostile attitudes and negative messages targeted toward their racial identity. Items were developed on basis of prior literature, vetted through expert feedback and cognitive interviews, and administered to 655 Asian American participants through Amazon Mechanical Turk. Exploratory factor analysis with a random subsample (n = 324) yielded a psychometrically robust preliminary measurement model consisting of 3 factors: Self-Negativity, Weakness Stereotypes, and Appearance Bias. Confirmatory factor analysis with a separate subsample (n = 331) indicated that the proposed correlated factors model was strongly consistent with the observed data. Factor determinacies were high and demonstrated that the specified items adequately measured their intended factors. Bifactor modeling further indicated that this multidimensionality could be univocally represented for the purpose of measurement, including the use of a mean total score representing a single continuum of internalized racism on which individuals vary. The IRAAS statistically predicted depressive symptoms, and demonstrated statistically significant correlations in theoretically expected directions with four dimensions of collective self-esteem. These results provide initial validity evidence supporting the use of the IRAAS to measure aspects of internalized racism in this population. Limitations and research implications are discussed. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  7. [The rapid development of child neuropsychology].

    Science.gov (United States)

    Billard, C; Jambaqué, I

    2008-05-01

    The past few years have seen important progress and new developments in the field of child neuropsychology. Children can exhibit acquired or learning disorders, but in all cases such deficits take place in a developmental trajectory that requires taking into account cerebral maturation and plasticity processes. Child neuropsychology finds its specificity in the perspective of developmental neuropsychology and has close connections with health and education. This article focuses on two pathologies - childhood epilepsies and developmental dyslexia - that highlight the recent progress in this specialty and its perspectives.

  8. Developing and validating rapid assessment instruments

    CERN Document Server

    Abell, Neil; Kamata, Akihito

    2009-01-01

    This book provides an overview of scale and test development. From conceptualization through design, data collection, analysis, and interpretation, critical concerns are identified and grounded in the increasingly sophisticated psychometric literature. Measurement within the health, social, and behavioral sciences is addressed, and technical and practical guidance is provided. Acknowledging the increasingly sophisticated contributions in social work, psychology, education, nursing, and medicine, the book balances condensation of complex conceptual challenges with focused recommendations for conceiving, planning, and implementing psychometric study. Primary points are carefully referenced and consistently illustrated to illuminate complicated or abstract principles. Basics of construct conceptualization and establishing evidence of validity are complimented with introductions to concept mapping and cross-cultural translation. In-depth discussion of cutting edge topics like bias and invariance in item responses...

  9. Intersection of suicidality and substance abuse among young Asian-American women: implications for developing interventions in young adulthood.

    Science.gov (United States)

    Hahm, Hyeouk Chris; Chang, Stephanie Tzu-Han; Tong, Hui Qi; Meneses, Michelle Ann; Yuzbasioglu, Rojda Filiz; Hien, Denise

    2014-01-01

    The purpose of this paper is to provide a review of the current literature uncovering specific factors associated with self-harm and suicidality among young Asian American women, as well as to present the Fractured Identity Model as a framework for understanding these factors. This paper offers concrete suggestions for the development of culturally competent interventions to target suicidality, substance abuse, and mental illness among young Asian American women. Empirical studies and theory-based papers featured in peer-reviewed journals between 1990 and 2014 were identified through scholarly databases, such as PubMed, MEDLINE, PsycINFO, JSTOR, and Google Scholar. We identified several factors associated with suicidality among young Asian American women: (1) family dynamics, or having lived in a household where parents practice "disempowering parenting styles," (2) substance use/abuse, and (3) untreated mental illness(es), which are exacerbated by the stigma and shame attached to seeking out mental health services. The Fractured Identity Model by Hahm et al. (2014) is presented as a possible pathway from disempowering parenting to suicidal and self-harm behaviors among this population, with substance abuse playing a significant mediating role. Research limitations/implications - Our review focused on Asian American women, substance use among Asian Americans, and mental health among Asian Americans. Literature that focused on Asians living in Asia or elsewhere outside of the USA was excluded from this review; the review was limited to research conducted in the USA and written in the English language. The complex interplay among Asian American culture, family dynamics, gender roles/expectations, and mental health justifies the development of a suicide and substance abuse intervention that is tailored to the culture- and gender-specific needs of Asian Pacific Islander young women. It is imperative for professionals in the fields of public health, mental health

  10. Intersection of suicidality and substance abuse among young Asian-American women: implications for developing interventions in young adulthood

    Science.gov (United States)

    Hahm, Hyeouk Chris; Chang, Stephanie Tzu-Han; Tong, Hui Qi; Meneses, Michelle Ann; Yuzbasioglu, Rojda Filiz; Hien, Denise

    2014-01-01

    Purpose The purpose of this paper is to provide a review of the current literature uncovering specific factors associated with self-harm and suicidality among young Asian American women, as well as to present the Fractured Identity Model as a framework for understanding these factors. This paper offers concrete suggestions for the development of culturally competent interventions to target suicidality, substance abuse, and mental illness among young Asian American women. Design/methodology/approach Empirical studies and theory-based papers featured in peer-reviewed journals between 1990 and 2014 were identified through scholarly databases, such as PubMed, MEDLINE, PsycINFO, JSTOR, and Google Scholar. Findings We identified several factors associated with suicidality among young Asian American women: (1) family dynamics, or having lived in a household where parents practice “disempowering parenting styles,” (2) substance use/abuse, and (3) untreated mental illness(es), which are exacerbated by the stigma and shame attached to seeking out mental health services. The Fractured Identity Model by Hahm et al. (2014) is presented as a possible pathway from disempowering parenting to suicidal and self-harm behaviors among this population, with substance abuse playing a significant mediating role. Research limitations/implications – Our review focused on Asian American women, substance use among Asian Americans, and mental health among Asian Americans. Literature that focused on Asians living in Asia or elsewhere outside of the USA was excluded from this review; the review was limited to research conducted in the USA and written in the English language. Practical implications The complex interplay among Asian American culture, family dynamics, gender roles/expectations, and mental health justifies the development of a suicide and substance abuse intervention that is tailored to the culture- and gender-specific needs of Asian Pacific Islander young women. It is

  11. The reasons for the rapid development of human technologies

    OpenAIRE

    Macfarlane, Alan

    2004-01-01

    While playing with a two-year-old girl in Australia, Alan Macfarlane explains one reason for the rapid development of humans, namely their curiosity and desire to experiment and to imagine and make new things.

  12. Asia-Pacific mussel watch: monitoring of butyltin contamination in coastal waters of Asian developing countries.

    Science.gov (United States)

    Sudaryanto, Agus; Takahashi, Shin; Monirith, In; Ismail, Ahmad; Muchtar, Muswerry; Zheng, Jinshu; Richardson, Bruce J; Subramanian, Annamalai; Prudente, Maricar; Hue, Nguyen Duc; Tanabe, Shinsuke

    2002-10-01

    Butyltin compounds (BTs) including mono-, di-, and tributyltin and total tin (sigmaSn), were determined in green mussels (Perna viridis) from various Asian developing countries, such as Cambodia, China (Hong Kong and southern China), Malaysia, India, Indonesia, the Philippines, and Vietnam, to elucidate the contamination status, distribution, and possible sources and to assess the risks on aquatic organisms and humans. Butyltin compounds were detected in green mussels collected from all the sampling location investigated, suggesting widespread contamination of BTs along the coastal waters of Asian developing countries. Among butyltin derivatives, tributyltin (TBT) was the predominant compound, indicating its ongoing usage and recent exposures in Asian coastal waters. Higher concentrations of BTs were found in mussels collected at locations with intensive maritime activities, implying that the usage of TBT as a biocide in antifouling paints was a major source of BTs. In addition, relatively high concentrations of BTs were observed in mussels from aquaculture areas in Hong Kong and Malaysia, as it has been reported in Thailand. With the recent improvement in economic status in Asia, it is probable that an increase in TBT usage will occur in aquaculture. Although contamination levels were generally low in mussel samples from most of the Asian developing countries, some of those from polluted areas in Hong Kong, India, Malaysia, the Philippines, and Thailand revealed levels comparable to those in developed nations. Furthermore, the concentrations of TBT in some mussels from polluted areas exceeded the threshold for toxic effects on organisms and estimated tolerable average residue levels as seafoods for human consumption. A significant correlation was observed between the concentrations of sigmaBTs and sigmaSn in mussels, and sigmaBTs were made up mostly 100% of sigmaSn in mussels taken from locations having intensive maritime/human activities. This suggests that

  13. Developing core competencies for monitoring and evaluation tracks in South Asian MPH programs.

    Science.gov (United States)

    Negandhi, Himanshu; Negandhi, Preeti; Tiwari, Ritika; Sharma, Anjali; Zodpey, Sanjay; Kulatilaka, Hemali; Tikyani, Sangeeta

    2015-08-05

    Monitoring and evaluation (M&E) provides vital information for decision-making and its structures, systems and processes are expected to be integrated throughout the life-cycle of public health programs. The acquisition of these skills should be developed in a structured manner and needs educational systems to identify core competencies in M&E teaching. This article presents our work on harmonizing M&E competencies for Masters level programs in the South Asian context and undertaking the global review of M&E track/ concentration offered in various Masters of Public Health (MPH) programs. Through an online search and snow-balling, we mapped institutions offering M&E tracks/ concentrations in Masters of Public Health (MPH) programs globally. We obtained detailed information about their M&E curriculum from university websites and brochures. The data on curricular contents was extracted and compiled. We analyzed the curricular contents using the framework for core competencies developed by the Association of Schools of Public Health (ASPH); and the Miller's triangle. This data was then used to inform a consultative exercise aimed at identifying core competencies for an M&E track/ concentration in MPH programs in the South Asian context. Our curricular review of M&E content within MPH programs globally showed that different domains or broad topic areas relating to M&E are covered differently across the programs. The quantitative sciences (Biostatistics and Epidemiology) and Health Policy and Management are covered in much greater depth than the other two domains (Social & Behavioral Sciences and Environmental Health Sciences). The identification of core competencies for an M&E track/ concentration in the South Asian context was undertaken through a consultative group exercise involving representation from 11 institutions across Bangladesh, India, Nepal and Sri Lanka. During the consultation, the group engaged in a focused discussion to reach consensus on a set of 15

  14. Asian river fishes in the Anthropocene: threats and conservation challenges in an era of rapid environmental change.

    Science.gov (United States)

    Dudgeon, D

    2011-12-01

    This review compares and contrasts the environmental changes that have influenced, or will influence, fishes and fisheries in the Yangtze and Mekong Rivers. These two rivers have been chosen because they differ markedly in the type and intensity of prevailing threats. The Mekong is relatively pristine, whereas the Three Gorges Dam on the Yangtze is the world's largest dam representing the apotheosis of environmental alteration of Asian rivers thus far. Moreover, it is situated at the foot of a planned cascade of at least 12 new dams on the upper Yangtze. Anthropogenic effects of dams and pollution of Yangtze fishes will be exacerbated by plans to divert water northwards along three transfer routes, in part to supplement the flow of the Yellow River. Adaptation to climate change will undoubtedly stimulate more dam construction and flow regulation, potentially causing perfect storm conditions for fishes in the Yangtze. China has already built dams along the upper course of the Mekong, and there are plans for as many as 11 mainstream dams in People's Democratic Republic (Laos) and Cambodia in the lower Mekong Basin. If built, they could have profound consequences for biodiversity, fisheries and human livelihoods, and such concerns have stalled dam construction. Potential effects of dams proposed for other rivers (such as Nujiang-Salween) are also cause for concern. Conservation or restoration measures to sustain some semblance of the rich fish biodiversity of Asian rivers can be identified, but their implementation may prove problematic in a context of increasing Anthropocene alteration of these ecosystems. © 2011 The Author. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles.

  15. Evidence for genetic association between East Asian and western North American Crataegus L. (Rosaceae) and rapid divergence of the eastern North American lineages based on multiple DNA sequences.

    Science.gov (United States)

    Lo, Eugenia Y Y; Stefanović, Sasa; Christensen, Knud Ib; Dickinson, Timothy A

    2009-05-01

    Phylogeographic relationships were constructed for 72 Old and New World Crataegus species using combinations of four chloroplast and up to five nuclear regions. Maximum parsimony, maximum likelihood, and Bayesian results yield consistent relationships among major lineages. The close associations of the East Asian and western North American species point toward ancient trans-Beringian migrations. Relationships among eastern North American species are poorly resolved and few groups are identified that are congruent with existing classifications. Scant variation and short internal branches among these species suggest rapid divergence associated with polyploidy and hybridization. Incongruence between the chloroplast and nuclear data, and morphology suggest hybrid origins of three species from an extinct European lineage (the male parent) and three different North American female parents. Europe and eastern North America are suggested as the most recent common areas for Crataegus; at least four dispersal events are inferred to explain the present distribution of the genus.

  16. Development of a Fluvial Egg Drift Simulator to evaluate the transport and dispersion of Asian carp eggs in rivers

    Science.gov (United States)

    Garcia, Tatiana; Jackson, P. Ryan; Murphy, Elizabeth A.; Valocchi, Albert J.; Garcia, Marcelo H.

    2013-01-01

    Asian carp are migrating towards the Great Lakes and are threatening to invade this ecosystem, hence there is an immediate need to control their population. The transport of Asian carp eggs in potential spawning rivers is an important factor in its life history and recruitment success. An understanding of the transport, development, and fate of Asian carp eggs has the potential to create prevention, management, and control strategies before the eggs hatch and develop the ability to swim. However, there is not a clear understanding of the hydrodynamic conditions at which the eggs are transported and kept in suspension. This knowledge is imperative because of the current assumption that suspension is required for the eggs to survive. Herein, FluEgg (Fluvial Egg Drift Simulator), a three-dimensional Lagrangian model capable of evaluating the influence of flow velocity, shear dispersion and turbulent diffusion on the transport and dispersal patterns of Asian carp eggs is presented. The model's variables include not only biological behavior (growth rate, density changes) but also the physical characteristics of the flow field, such as mean velocities and eddy diffusivities. The performance of the FluEgg model was evaluated using observed data from published flume experiments conducted in China with water-hardened Asian carp eggs as subjects. FluEgg simulations show a good agreement with the experimental data. The model was also run with observed data from the Sandusky River in Ohio to provide a real-world demonstration case. This research will support the identification of critical hydrodynamic conditions (e.g., flow velocity, depth, and shear velocity) to maintain eggs in suspension, assist in the evaluation of suitable spawning rivers for Asian carp populations and facilitate the development of prevention, control and management strategies for Asian carp species in rivers and water bodies.

  17. Development and implementation of a rapid real-time polymerase ...

    African Journals Online (AJOL)

    Assays which use real-time polymerase chain reaction (PCR) technology can be developed for the rapid identification of genetic sequences carried by waterborne pathogens. Rand Water has established facilities within which a selection of PCR assays will be developed. This paper reports on the optimisation and ...

  18. The Interpersonal Shame Inventory for Asian Americans: Scale Development and Psychometric Properties

    Science.gov (United States)

    Wong, Y. Joel; Kim, Bryan S. K.; Nguyen, Chi P.; Cheng, Janice Ka Yan; Saw, Anne

    2016-01-01

    This article reports the development and psychometric properties of the Interpersonal Shame Inventory (ISI), a culturally salient and clinically relevant measure of interpersonal shame for Asian Americans. Across 4 studies involving Asian American college students, the authors provided evidence for this new measure’s validity and reliability. Exploratory factor analyses and confirmatory factor analyses provided support for a model with 2 correlated factors: external shame (arising from concerns about others’ negative evaluations) and family shame (arising from perceptions that one has brought shame to one’s family), corresponding to 2 subscales: ISI-E and ISI-F, respectively. Evidence for criterion-related, concurrent, discriminant, and incremental validity was demonstrated by testing the associations between external shame and family shame and immigration/international status, generic state shame, face concerns, thwarted belongingness, perceived burdensomeness, self-esteem, depressive symptoms, and suicide ideation. External shame and family shame also exhibited differential relations with other variables. Mediation findings were consistent with a model in which family shame mediated the effects of thwarted belongingness on suicide ideation. Further, the ISI subscales demonstrated high alpha coefficients and test–retest reliability. These findings are discussed in light of the conceptual, methodological, and clinical contributions of the ISI. PMID:24188650

  19. The interpersonal shame inventory for Asian Americans: scale development and psychometric properties.

    Science.gov (United States)

    Wong, Y Joel; Kim, Bryan S K; Nguyen, Chi P; Cheng, Janice Ka Yan; Saw, Anne

    2014-01-01

    This article reports the development and psychometric properties of the Interpersonal Shame Inventory (ISI), a culturally salient and clinically relevant measure of interpersonal shame for Asian Americans. Across 4 studies involving Asian American college students, the authors provided evidence for this new measure's validity and reliability. Exploratory factor analyses and confirmatory factor analyses provided support for a model with 2 correlated factors: external shame (arising from concerns about others' negative evaluations) and family shame (arising from perceptions that one has brought shame to one's family), corresponding to 2 subscales: ISI-E and ISI-F, respectively. Evidence for criterion-related, concurrent, discriminant, and incremental validity was demonstrated by testing the associations between external shame and family shame and immigration/international status, generic state shame, face concerns, thwarted belongingness, perceived burdensomeness, self-esteem, depressive symptoms, and suicide ideation. External shame and family shame also exhibited differential relations with other variables. Mediation findings were consistent with a model in which family shame mediated the effects of thwarted belongingness on suicide ideation. Further, the ISI subscales demonstrated high alpha coefficients and test-retest reliability. These findings are discussed in light of the conceptual, methodological, and clinical contributions of the ISI. (c) 2014 APA, all rights reserved.

  20. Methods for Rapid Screening in Woody Plant Herbicide Development

    Directory of Open Access Journals (Sweden)

    William Stanley

    2014-07-01

    Full Text Available Methods for woody plant herbicide screening were assayed with the goal of reducing resources and time required to conduct preliminary screenings for new products. Rapid screening methods tested included greenhouse seedling screening, germinal screening, and seed screening. Triclopyr and eight experimental herbicides from Dow AgroSciences (DAS 313, 402, 534, 548, 602, 729, 779, and 896 were tested on black locust, loblolly pine, red maple, sweetgum, and water oak. Screening results detected differences in herbicide and species in all experiments in much less time (days to weeks than traditional field screenings and consumed significantly less resources (<500 mg acid equivalent per herbicide per screening. Using regression analysis, various rapid screening methods were linked into a system capable of rapidly and inexpensively assessing herbicide efficacy and spectrum of activity. Implementation of such a system could streamline early-stage herbicide development leading to field trials, potentially freeing resources for use in development of beneficial new herbicide products.

  1. Economic Growth of a Rapidly Developing Economy: Theoretical Formulation

    Directory of Open Access Journals (Sweden)

    Oleg Sergeyevich Sukharev

    2016-06-01

    Full Text Available The subject matter of the article is the description of economic growth. Modern economy is characterized by a high rate of changes. These changes are the limiting parameters of modern development, which requires a modification of the basic models of growth, the substantiation of the expediency and necessity of a rapid development strategy. In a simple mathematical form, the statement of the problem of economic growth in the “green economy” is examined, in which the costs of environmental measures are not considered a priori as hampering economic development (as it is common for a number of modern neoclassical and neo-Keynesian growth models. The methodological basis of the article are the econometric approach and modelling method. The article has a theoretical character. The main hypothesis supposes that the rapid development strategy cannot make an adequate development strategy under certain conditions, but may be acceptable in other its specific conditions. In this sense, the important growth conditions are the availability of resources, the effectiveness of institutions and the current economic structure, the technological effectiveness of economy, as well as the conditions of technological development (“green economy” and the path of such development. In the article, on the theoretical level of analysis, the substantiation of the adequacy of the rapid development strategy for an economic system is given, whose goal is to achieve the standard of living of the countryleader. Based on the assumptions introduced, the period for which the rapid development strategy might be implemented and the economic lag of the country might be reduced from the country-leader is determined. The conditions that ensure the impact of innovations on the rate of economic development are summarized. The introduced range of dependencies and relations can be useful for the elaboration of the theory of innovation development and for the formation of a new

  2. Development and implementation of a rapid real-time polymerase ...

    African Journals Online (AJOL)

    6–10 May 2012. * To whom all correspondence should be addressed. ☎ +27 16 430-8403; fax: +27 16 455-2055; e-mail: nleat@randwater.co.za. Development and implementation of a rapid real-time polymerase chain reaction assay for the detection of toxigenic Vibrio cholerae in water. Neil Leat* and Monique Grundlingh.

  3. Development of a reverse transcription quantitative polymerase chain reaction-based assay for broad coverage detection of African and Asian Zika virus lineages.

    Science.gov (United States)

    Yang, Yang; Wong, Gary; Ye, Baoguo; Li, Shihua; Li, Shanqin; Zheng, Haixia; Wang, Qiang; Liang, Mifang; Gao, George F; Liu, Lei; Liu, Yingxia; Bi, Yuhai

    2017-06-01

    The Zika virus (ZIKV) is an arbovirus that has spread rapidly worldwide within recent times. There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome (GBS) and microcephaly in humans. The ZIKV is genetically diverse and can be separated into Asian and African lineages. A rapid, sensitive, and specific assay is needed for the detection of ZIKV across various pandemic regions. So far, the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains. To this end, we have developed a one-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay based on conserved sequences in the ZIKV envelope (E) gene. The detection limit of the assay was determined to be five RNA transcript copies and 2.94 × 10-3 50% tissue culture infectious doses (TCID50) of live ZIKV per reaction. The assay was highly specific and able to detect five different ZIKV strains covering the Asian and African lineages without nonspecific amplification, when tested against other flaviviruses. The assay was also successful in testing for ZIKV in clinical samples. Our assay represents an improvement over the current methods available for the detection ZIKV and would be valuable as a diagnostic tool in various pandemic regions.

  4. "Clutching a knifeblade": human rights and development from Asian women's perspective.

    Science.gov (United States)

    Sancho-liao, N

    1993-06-01

    A brief, vivid portrait of the human rights conditions for women in Asia was presented: "kapit sa patalim" or utter despair, urban migration, export processing zones, tourism and prostitution, political repression, and military sexual slavery. Advocates of women's human rights for Asian women must contend with patriarchal and male-dominated systems that oppress and exploit women to a much greater extent than men. Liberation from these systems and the domination and exploitation by wealthier nations must be a goal of a new economic world order. Unjust and repressive structures must be destroyed, and equitable distribution of wealth and democracy and popular initiatives promoted. The status of women must be raised to coequal status with men. The most important objective of human rights advocates should be the empowerment of women at the individual, community, national, regional, and international level. The Asian Women's Human Rights Council was established as an addition to 3 already operating regional commissions of women's organizations. The aim was not just to describe women as victims, but to pressure development activity to account for women's human rights. Sex tribunals have been scheduled between 1993 and 1994 to address the following issues: 1) sex trafficking (Japan, May 1993); 2) violence against women (Pakistan, December 1993); 3) militarism, environment, and violence against women (Korea, March 1994); 4) crimes of development against women in Asia (India); 5) religion and violence against women (Malaysia, 1994); and 6) indigenous women (December 1994). Women were victims when Filipino domestic workers were stranded and raped in Iraq during the chaos of war, when girls from landless peasant families migrated near Clark Air Force Base to earn a living as prostitutes for US servicemen, when women were forced to work 36-hour shifts in foreign-owned garment factories in Bataan, when women migrated for work, and when women were abused and battered in

  5. Rapid retreat of the East Asian summer monsoon in the middle Holocene and a millennial weak monsoon interval at 9 ka in northern China

    Science.gov (United States)

    Dong, Jinguo; Shen, Chuan-Chou; Kong, Xinggong; Wu, Chung-Che; Hu, Hsun-Ming; Ren, Haojia; Wang, Yi

    2018-01-01

    Knowledge of hydroclimatic dynamics in the East Asian monsoon region during the Holocene was hindered by few absolutely-dated and decadally-resolved proxy records in northern China. Here we present replicated carbonate δ18O records of six stalagmites with sub-decadal to multi-decadal resolutions from the Lianhua cave to reveal a detailed evolution of the East Asian Summer Monsoon (EASM) intensity in northern China since 11.5 thousand years before present (ka BP, before 1950 CE). Our composite record shows that solar forcing dominated hydroclimatic changes regionally, including an intensified monsoon at the Holocene Optimum from the termination of Younger Dryas to 6.5 ka BP, and a subsequent multi-millennial weakening monsoon, that agrees with cave records in central and southern China. However, the EASM has retreated southwards more rapidly than the Indian summer monsoon after ∼6.5 ka BP, resulting in aridity conditions occurring at 4.0 ka BP in northern China, which is almost 2000-year earlier than that in central and southern China. This north-south asynchroneity is likely related to the different regional responses among the coupling of the EASM, Indian summer monsoon, the solar forcing, and the differences in thermal forcing due to complex geographical configurations. In addition, a relative enrichment of 1‰ in 18O data of the Lianhua record from 9.5 to 8.1 ka BP shows that the Holocene Optimum was punctuated by a millennial-long weakening monsoon interval, which is not registered among previous cave records in central and southern China. The fresh water-induced cold climate conditions in the North Atlantic region could create stronger East Asian winter monsoon, and induce a weakened EASM and a southward shift of rain belt in northern China. Therefore, it shall not be surprised that there are strong heterogeneities among regional hydroclimatic conditions across monsoonal China, given the complex interplay between external and internal forcing mechanisms

  6. Drought risk management for increased cereal production in Asian Least Developed Countries

    Directory of Open Access Journals (Sweden)

    Md. Rezaul Karim

    2015-03-01

    Full Text Available Drought stress is a serious abiotic factor inhibiting nutrient acquisition by roots and limiting cereal crop production in Asian Least Developed Countries (LDCs. Many studies revealed that balanced fertilization can improve photosynthetic activity by stabilizing superoxide dismutase (SOD activity, improve proline, abscisic acid (ABA and make the crop drought tolerant with efficient root system and finally improve crop yield. To mitigate drought stress, along with the usage of resistant and efficient genotypes, soil and foliar applications of macro- and micro-nutrients are being used in Asia. According to drought sensitivity index, the efficient genotypes are found more drought-tolerant than the inefficient ones. Studies revealed that irrigation alone is not sufficient to obtain satisfactory grain yield without balanced fertilization. At drought stress, the efficient genotypes accumulate higher quantities of ABA and proline, and exhibit higher activities of SOD, as compared with inefficient genotypes through greater nutrient accumulation by their longer and thinner root systems with high efficiency. Under severe drought with low nutritional status, the rate of photosynthesis, particularly water-use efficiency (WUE increases in the efficient genotypes than in the inefficient ones. Consequently, these physiological and morphological parameters result in better yield performance by efficient use of water. Under drought, soil application of NPK along with foliar application of zinc (Zn, boron (B and manganese (Mn increase grain yield as well as micronutrients concentration of the grain. The rate of photosynthesis, pollen viability, number of fertile spikes, number of grains per spike, and WUE are increased by late foliar application of these micronutrients. This indicates that, by increasing WUE foliar application of Zn, B and Mn at booting to anthesis can reduce the harmful effects of drought that often occur during the late stages of cereal

  7. The Role of Structural Change in the Economic Development of Asian Economies

    NARCIS (Netherlands)

    Foster-McGregor, N.; Verspagen, Bart

    2016-01-01

    In this paper, we combine data on gross domestic product (GDP) per capita and sectoral employment shares to undertake a decomposition of GDP per capita growth for a sample of 43 Asian and non-Asian economies. We decompose income changes into three components: (i) changes in labor productivity within

  8. A Social Cognitive Examination of East Asian American Career Development: Contextual Factors Influencing Career Choice

    Science.gov (United States)

    Liu, Jane

    2012-01-01

    Despite their educational and economic achievements in the United States, Asian Americans continue to be occupationally segregated in the labor force. Asian Americans are overrepresented in mathematics, engineering and biological sciences while underrepresented in field such as education, humanities, social and behavioral sciences (Bureau of Labor…

  9. The role of the Asian winter monsoon in the rapid propagation of abrupt climate changes during the last deglaciation

    Science.gov (United States)

    Chu, Guoqiang; Sun, Qing; Zhu, Qingzeng; Shan, Yabing; Shang, Wenyu; Ling, Yuan; Su, Youliang; Xie, Manman; Wang, Xishen; Liu, Jiaqi

    2017-12-01

    High-resolution temperature records spanning the last deglaciation from low latitudes are scarce; however, they are important for understanding the rapid propagation of abrupt climate events throughout the Northern Hemisphere and the tropics. Here, we present a branched GDGTs-based temperature reconstruction from the sediments of Maar Lake Huguangyan in tropical China. The record reveals that the mean temperature during the Oldest Dryas was 17.8 °C, which was followed by a two-step increase of 2-3 °C to the Bølling-Allerød, a decrease to 19.8 °C during the Younger Dryas, and a rapid warming at the onset of the Holocene. The Oldest Dryas was about 2 °C warmer than the Younger Dryas. The reconstructed temperature was weighted towards the wintertime since the lake is monomictic and the mixing process in winter supplies nutrients from the lake bottom to the entire water column, greatly promoting biological productivity. In addition, the winter-biased temperature changes observed in the study are more distinctive than the summer-biased temperature records from extra-tropical regions of East Asia. This implies that the temperature decreases during abrupt climatic events were mainly a winter phenomenon. Within the limits of the dating uncertainties, the broadly similar pattern of winter-weighted temperature change observed in both tropical Lake Huguangyan and in Greenland ice cores indicates the occurrence of tightly-coupled interactions between high latitude ice sheets and land areas in the tropics. We suggest that the winter monsoon (especially cold surges) could play an important role in the rapid transmission of the temperature signal from the Arctic to the tropics.

  10. Seasonal abundance and development of the Asian longhorned beetle and natural enemy prevalence in different forest types in China

    Science.gov (United States)

    Houping Liu; Leah S. Bauer; Tonghai Zhao; Ruitong Gao; Therese M. Poland

    2016-01-01

    Seasonal abundance and population development of the Asian longhorned beetle (ALB), Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), and prevalence of its natural enemies were studied on Hankow willow (Salix matsudana Koidz.) at an urban forest site (Anci) and a rural forest site (Tangerli) in Hebei province...

  11. Vocational Guidance in Manpower Development: Papers Presented at the Asian Regional Conference-Workshop (Manila, Philippines, 1977).

    Science.gov (United States)

    ERIC Clearinghouse on Counseling and Personnel Services, Ann Arbor, MI.

    The unemployment of youth and adults has surfaced as a problem and a challenge to Asian Third World governments. Industry and business continue to experience a shortage of skilled and technical manpower. A manpower development conference was held in the Philippines on topics which included: (1) the role of private educational institutions in…

  12. DEVELOPMENT OF THE PRIEST RAPIDS TURBINE UPGRADE PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    DeBolt, Donald; Richmond, Marshall C.; Donelson, Richard K.; Strickler, Brad; Weisbeck, Molly

    2015-07-14

    The Priest Rapids Dam is located on the Columbia River and is operated by Public Utility District No. 2 of Grant County, WA (GCPUD). In operation since 1959, GCPUD decided that a major upgrade was necessary. As with other hydroelectric facilities on the Columbia River, improving fish passage at Priest Rapids Dam is of great importance for salmon and steelhead populations in the Pacific Northwest. Consequently, GCPUD established the Priest Rapids Turbine Upgrade Project to extend the life of the units, increase efficiency and power production, and improve fish-passage. The Priest Rapids powerhouse is equipped with 10 vertical Kaplan turbines with runner diameters of 7.21 m operating under net heads varying from 18 m to 27 m. The scope of the project included a design competition involving three turbine manufacturers providing up to two designs for evaluation. Selection of the replacement design was determined by the lowest evaluated price based on model test results conducted at an independent laboratory (Ecole Polytechnique Federale de Lausanne in Lausanne Switzerland) and a biological performance score determined by a newly developed Biological Performance Assessment (BioPA) performed by Pacific Northwest National Laboratory in Richland, WA. In the paper, the hydraulic design challenges are reviewed, in addition to comparisons of designs evaluated during the competitive model test program and biological assessment. The paper also provides a description of the process followed by GCPUD, and how the evaluation criteria influenced the development and the finally selected solution.

  13. Obesity and Dyslipidemia in South Asians

    Directory of Open Access Journals (Sweden)

    Anoop Misra

    2013-07-01

    Full Text Available Obesity and dyslipidemia are emerging as major public health challenges in South Asian countries. The prevalence of obesity is more in urban areas than rural, and women are more affected than men. Further, obesity in childhood and adolescents is rising rapidly. Obesity in South Asians has characteristic features: high prevalence of abdominal obesity, with more intra-abdominal and truncal subcutaneous adiposity than white Caucasians. In addition, there is greater accumulation of fat at “ectopic” sites, namely the liver and skeletal muscles. All these features lead to higher magnitude of insulin resistance, and its concomitant metabolic disorders (the metabolic syndrome including atherogenic dyslipidemia. Because of the occurrence of type 2 diabetes, dyslipidemia and other cardiovascular morbidities at a lower range of body mass index (BMI and waist circumference (WC, it is proposed that cut-offs for both measures of obesity should be lower (BMI 23–24.9 kg/m2 for overweight and ≥25 kg/m2 for obesity, WC ≥80 cm for women and ≥90 cm for men for abdominal obesity for South Asians, and a consensus guideline for these revised measures has been developed for Asian Indians. Increasing obesity and dyslipidemia in South Asians is primarily driven by nutrition, lifestyle and demographic transitions, increasingly faulty diets and physical inactivity, in the background of genetic predisposition. Dietary guidelines for prevention of obesity and diabetes, and physical activity guidelines for Asian Indians are now available. Intervention programs with emphasis on improving knowledge, attitude and practices regarding healthy nutrition, physical activity and stress management need to be implemented. Evidence for successful intervention program for prevention of childhood obesity and for prevention of diabetes is available for Asian Indians, and could be applied to all South Asian countries with similar cultural and lifestyle profiles. Finally, more

  14. Obesity and Dyslipidemia in South Asians

    Science.gov (United States)

    Misra, Anoop; Shrivastava, Usha

    2013-01-01

    Obesity and dyslipidemia are emerging as major public health challenges in South Asian countries. The prevalence of obesity is more in urban areas than rural, and women are more affected than men. Further, obesity in childhood and adolescents is rising rapidly. Obesity in South Asians has characteristic features: high prevalence of abdominal obesity, with more intra-abdominal and truncal subcutaneous adiposity than white Caucasians. In addition, there is greater accumulation of fat at “ectopic” sites, namely the liver and skeletal muscles. All these features lead to higher magnitude of insulin resistance, and its concomitant metabolic disorders (the metabolic syndrome) including atherogenic dyslipidemia. Because of the occurrence of type 2 diabetes, dyslipidemia and other cardiovascular morbidities at a lower range of body mass index (BMI) and waist circumference (WC), it is proposed that cut-offs for both measures of obesity should be lower (BMI 23–24.9 kg/m2 for overweight and ≥25 kg/m2 for obesity, WC ≥80 cm for women and ≥90 cm for men for abdominal obesity) for South Asians, and a consensus guideline for these revised measures has been developed for Asian Indians. Increasing obesity and dyslipidemia in South Asians is primarily driven by nutrition, lifestyle and demographic transitions, increasingly faulty diets and physical inactivity, in the background of genetic predisposition. Dietary guidelines for prevention of obesity and diabetes, and physical activity guidelines for Asian Indians are now available. Intervention programs with emphasis on improving knowledge, attitude and practices regarding healthy nutrition, physical activity and stress management need to be implemented. Evidence for successful intervention program for prevention of childhood obesity and for prevention of diabetes is available for Asian Indians, and could be applied to all South Asian countries with similar cultural and lifestyle profiles. Finally, more research on

  15. Obesity and dyslipidemia in South Asians.

    Science.gov (United States)

    Misra, Anoop; Shrivastava, Usha

    2013-07-16

    Obesity and dyslipidemia are emerging as major public health challenges in South Asian countries. The prevalence of obesity is more in urban areas than rural, and women are more affected than men. Further, obesity in childhood and adolescents is rising rapidly. Obesity in South Asians has characteristic features: high prevalence of abdominal obesity, with more intra-abdominal and truncal subcutaneous adiposity than white Caucasians. In addition, there is greater accumulation of fat at "ectopic" sites, namely the liver and skeletal muscles. All these features lead to higher magnitude of insulin resistance, and its concomitant metabolic disorders (the metabolic syndrome) including atherogenic dyslipidemia. Because of the occurrence of type 2 diabetes, dyslipidemia and other cardiovascular morbidities at a lower range of body mass index (BMI) and waist circumference (WC), it is proposed that cut-offs for both measures of obesity should be lower (BMI 23-24.9 kg/m(2) for overweight and ≥ 25 kg/m(2) for obesity, WC ≥ 80 cm for women and ≥ 90 cm for men for abdominal obesity) for South Asians, and a consensus guideline for these revised measures has been developed for Asian Indians. Increasing obesity and dyslipidemia in South Asians is primarily driven by nutrition, lifestyle and demographic transitions, increasingly faulty diets and physical inactivity, in the background of genetic predisposition. Dietary guidelines for prevention of obesity and diabetes, and physical activity guidelines for Asian Indians are now available. Intervention programs with emphasis on improving knowledge, attitude and practices regarding healthy nutrition, physical activity and stress management need to be implemented. Evidence for successful intervention program for prevention of childhood obesity and for prevention of diabetes is available for Asian Indians, and could be applied to all South Asian countries with similar cultural and lifestyle profiles. Finally, more research on

  16. A competing risk analysis of sequential complication development in Asian type 2 diabetes mellitus patients.

    Science.gov (United States)

    Cheng, Li-Jen; Chen, Jeng-Huei; Lin, Ming-Yen; Chen, Li-Chia; Lao, Chun-Huan; Luh, Hsing; Hwang, Shang-Jyh

    2015-10-28

    This retrospective cohort study investigated the progression risk of sequential complication in Asian type 2 diabetes (T2D) patients using the Taiwan Pay-for-Performance Diabetes Registry and claim data from November 2003 to February 2009. 226,310 adult T2D patients without complication were followed from diagnosis to complications, including myocardial infarction (MI), other ischemic heart disease (IHD), congestive heart failure (CHF), stroke, chronic kidney disease (CKD), retinopathy, amputation, death or to the end of study. Cumulative incidences (CIs) of first and second complications were analyzed in 30 and 4 years using the cumulative incidence competing risk method. IHD (29.8%), CKD (24.5%) and stroke (16.0%) are the most common first complications. The further development of T2D complications depends on a patient's existing complication profiles. Patients who initially developed cardiovascular complications had a higher risk (9.2% to 24.4%) of developing IHD or CKD, respectively. All-cause mortality was the most likely consequence for patients with a prior MI (12.0%), so as stroke in patients with a prior MI (10.8%) or IHD (8.9%). Patients with CKD had higher risk of developing IHD (16.3%), stroke (8.9%) and all-cause mortality (8.7%) than end-stage renal disease (4.0%). Following an amputation, patients had a considerable risk of all-cause mortality (42.1%).

  17. Onboard Radar Processing Development for Rapid Response Applications

    Science.gov (United States)

    Lou, Yunling; Chien, Steve; Clark, Duane; Doubleday, Josh; Muellerschoen, Ron; Wang, Charles C.

    2011-01-01

    We are developing onboard processor (OBP) technology to streamline data acquisition on-demand and explore the potential of the L-band SAR instrument onboard the proposed DESDynI mission and UAVSAR for rapid response applications. The technology would enable the observation and use of surface change data over rapidly evolving natural hazards, both as an aid to scientific understanding and to provide timely data to agencies responsible for the management and mitigation of natural disasters. We are adapting complex science algorithms for surface water extent to detect flooding, snow/water/ice classification to assist in transportation/ shipping forecasts, and repeat-pass change detection to detect disturbances. We are near completion of the development of a custom FPGA board to meet the specific memory and processing needs of L-band SAR processor algorithms and high speed interfaces to reformat and route raw radar data to/from the FPGA processor board. We have also developed a high fidelity Matlab model of the SAR processor that is modularized and parameterized for ease to prototype various SAR processor algorithms targeted for the FPGA. We will be testing the OBP and rapid response algorithms with UAVSAR data to determine the fidelity of the products.

  18. East Asian adolescents' ethnic identity development and cultural integration: A qualitative investigation.

    Science.gov (United States)

    Yoon, Eunju; Adams, Kristen; Clawson, Angela; Chang, Hanna; Surya, Shruti; Jérémie-Brink, Gihane

    2017-01-01

    Drawing on the current conceptualization of acculturation/enculturation as bilinear, multidimensional processes proceeding in interaction with surrounding contexts, this study examined ethnic identity development and cultural integration of 13 adolescents from East Asian immigrant families. Five domains emerged via the Consensual Qualitative Research method: ethnic/cultural identity and socialization; bicultural living; racial context-racism and stereotypes; family context-parental expectation; and peer context-friendship/dating. Overall, the participants experienced a cultural split and discontinuity between the 2 worlds of home and ethnic community versus school and society in general. They received strong ethnic socialization messages from family and ethnic community. Although most participants experienced hurtful racial discrimination, they used passive coping (e.g., dismiss, minimize, defend perpetrators). The model minority stereotype was prevalent and deeply engrained in many aspects of their lives including ethnic identity development, cultural socialization messages from mainstream society, discrimination experiences, and academic/occupational demands imposed by self, parents, peers, and society. Although they appreciated parents' high expectations of academic/occupational success, they felt pressured and desired to have space and independence. Friendship/dating patterns reflected ethnic identity development as well as contextual influence. Implications for research and practice are discussed. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  19. Postpartum development of type 1 diabetes in Asian Indian women with gestational diabetes

    Directory of Open Access Journals (Sweden)

    Ranjit Unnikrishnan

    2016-01-01

    Full Text Available Aim: To study the postpartum conversion of gestational diabetes mellitus (GDM to different types of diabetes among Asian Indian women. Materials and Methods: Using data from electronic medical records, 418 women with GDM seen at a tertiary diabetes care center for diabetes in Chennai in South India between 1991 and 2014 were evaluated for development of diabetes postpartum. Results: Of the 418 GDM women followed up postpartum, 388 progressed to diabetes. Of these 359 (92.5% developed type 2 diabetes (T2DM and 29 women (7.5% developed type 1 diabetes (T1DM. The median time to development of T1DM was 2 years (interquartile range 2 [IQR] while for T2DM it was 5 years (IQR 6. Women who developed T1DM had significantly lower mean body mass index (BMI (20.4 ± 2.8 vs. 27.5 ± 4.4 kg/m 2 , P = 0.001, and higher fasting plasma glucose (222 ± 105 vs. 165 ± 62 mg/dl P = 0.008 and glycated hemoglobin levels (10.2 ± 2.7 vs. 8.5 ± 2.1% P < 0.001 compared to those who developed T2DM. Glutamic acid decarboxylase (GAD autoantibodies were present in 24/29 (82.7% of women who developed T1DM. Conclusion: A small but significant proportion of women with GDM progress to T1DM postpartum. Measurement of GAD antibodies in leaner women with more severe diabetes could help to identify women who are likely to develop T1DM and thus prevent their presentation with acute hyperglycemic emergencies after delivery.

  20. Assessing the costs of municipal solid waste treatment technologies in developing Asian countries.

    Science.gov (United States)

    Aleluia, João; Ferrão, Paulo

    2017-11-01

    The management of municipal solid waste (MSW) is one of the main costs incurred by local authorities in developing countries. According to some estimates, these costs can account for up to 50% of city government budgets. It is therefore of importance that policymakers, urban planners and practitioners have an adequate understanding of what these costs consist of, from collection to final waste disposal. This article focuses on a specific stage of the MSW value chain, the treatment of waste, and it aims to identify cost patterns associated with the implementation and operation of waste treatment approaches in developing Asian countries. An analysis of the capital (CAPEX) and operational expenditures (OPEX) of a number of facilities located in countries of the region was conducted based on a database gathering nearly 100 projects and which served as basis for assessing four technology categories: composting, anaerobic digestion (AD), thermal treatment, and the production of refuse-derived fuel (RDF). Among these, it was found that the least costly to invest, asa function of the capacity to process waste, are composting facilities, with an average CAPEX per ton of 21,493 USD 2015 /ton. Conversely, at the upper end featured incineration plants, with an average CAPEX of 81,880 USD 2015 /ton, with this treatment approach ranking by and large as the most capital intensive of the four categories assessed. OPEX figures of the plants, normalized and analyzed in the form of OPEX/ton, were also found to be higher for incineration than for biological treatment methods, although on this component differences amongst the technology groups were less pronounced than those observed for CAPEX. While the results indicated the existence of distinct cost implications for available treatment approaches in the developing Asian context, the analysis also underscored the importance of understanding the local context asa means to properly identify the cost structure of each specific plant

  1. Rapid development of fungicide resistance by Sclerotinia homoeocarpa on turfgrass.

    Science.gov (United States)

    Jo, Young-Ki; Chang, Seog Won; Boehm, Michael; Jung, Geunhwa

    2008-12-01

    Dollar spot, caused by Sclerotinia homoeocarpa, is the most prevalent and economically important turfgrass disease in North America. Increasing levels of fungicide resistance, coupled with tightening environmental scrutiny of existing fungicides, has left fewer options for managing dollar spot. More knowledge about S. homoeocarpa populations is needed to improve dollar spot management strategies, especially with respect to minimizing the development of fungicide resistance. Population diversity of S. homoeocarpa was examined using inter-simple sequence repeat markers and vegetative compatibility assays. Two subgroups were found in S. homoeocarpa field populations on both fairway and putting green turfgrass at a research field in Wisconsin. These subgroups were genetically different, vegetatively incompatible, and had different fungicide sensitivities. The frequency of the two genetic subgroups differed significantly between the fairway and putting green, but was uniform within the fairway or within the green. Population dynamics of S. homoeocarpa in response to two systemic fungicides (thiophanate-methyl and propiconazole) were assessed based on in vitro fungicide sensitivity. Dynamics of S. homoeocarpa populations depended on the presence of fungicide-resistant isolates in the initial populations before fungicide applications and changed rapidly after fungicide applications. Shifting of the population toward propiconazole resistance was gradual, whereas thiophanate-methyl resistance developed rapidly in the population. In conclusion, field populations of S. homoeocarpa containing genetically distinct, vegetatively incompatible groups were different on turfgrass that was managed differently, and they were changed rapidly after exposure to fungicides.

  2. Modeling of subglacial hydrological development following rapid supraglacial lake drainage

    Science.gov (United States)

    Dow, C. F.; Kulessa, B.; Rutt, I. C.; Tsai, V. C.; Pimentel, S.; Doyle, S. H.; van As, D.; Lindbäck, K.; Pettersson, R.; Jones, G. A.; Hubbard, A.

    2015-06-01

    The rapid drainage of supraglacial lakes injects substantial volumes of water to the bed of the Greenland ice sheet over short timescales. The effect of these water pulses on the development of basal hydrological systems is largely unknown. To address this, we develop a lake drainage model incorporating both (1) a subglacial radial flux element driven by elastic hydraulic jacking and (2) downstream drainage through a linked channelized and distributed system. Here we present the model and examine whether substantial, efficient subglacial channels can form during or following lake drainage events and their effect on the water pressure in the surrounding distributed system. We force the model with field data from a lake drainage site, 70 km from the terminus of Russell Glacier in West Greenland. The model outputs suggest that efficient subglacial channels do not readily form in the vicinity of the lake during rapid drainage and instead water is evacuated primarily by a transient turbulent sheet and the distributed system. Following lake drainage, channels grow but are not large enough to reduce the water pressure in the surrounding distributed system, unless preexisting channels are present throughout the domain. Our results have implications for the analysis of subglacial hydrological systems in regions where rapid lake drainage provides the primary mechanism for surface-to-bed connections.

  3. Conflict between conservation and development: cash forest encroachment in Asian elephant distributions.

    Science.gov (United States)

    Liu, Peng; Wen, Hui; Harich, Franziska K; He, Changhuan; Wang, Lanxin; Guo, Xianming; Zhao, Jianwei; Luo, Aidong; Yang, Hongpei; Sun, Xiao; Yu, Yang; Zheng, Shaobo; Guo, Jing; Li, Li; Zhang, Li

    2017-08-03

    Over the last 4 decades, China has undergone major economic development, resulting in considerable impacts on its wildlife populations and habitats. It is essential to quantify the conflict between development and conservation to assist with policy-making because forestry policies and market trends affected indirectly the distribution of Asian elephants. Here, we mapped the historical distribution of elephants versus human land use. Elephant distributions appear to occur in unbroken natural forests only. However, over the 40-year period, the distribution ranges have become smaller and fragmented, with natural forest area also declining by 16%. The monoculture of cash trees is encroaching on natural forests. Over the past 10 years, rubber plantations have become concentrated in the south, with extensive natural forests and scattered rubber farms being converted to tea plantations, due to changes in governmental policies and product prices. Through mapping the spatial changes in the distribution of rubber and tea plantations, our study is expected to help local managers to incorporate the needs of endangered elephants through creating space when planning plantations, especially in Xishuangbanna and the south part of Pu'er. In conclusion, restoring elephant habitat and establishing ecological corridors are critical for the survival of elephants in this region.

  4. Effects of Fetal Exposure to Asian Sand Dust on Development and Reproduction in Male Offspring.

    Science.gov (United States)

    Yoshida, Seiichi; Ichinose, Takamichi; Arashidani, Keiichi; He, Miao; Takano, Hirohisa; Shibamoto, Takayuki

    2016-11-23

    In recent experimental studies, we reported the aggravating effects of Asian sand dust (ASD) on male reproduction in mice. However, the effects of fetal ASD exposure on male reproduction have not been investigated. The present study investigated the effects of fetal ASD exposure on reproduction in male offspring. Using pregnant CD-1 mice, ASD was administered intratracheally on days 7 and 14 of gestation, and the reproduction of male offspring was determined at 5, 10, and 15 weeks after birth. The secondary sex ratio was significantly lower in the fetal ASD-exposed mice than in the controls. Histologic examination showed partial vacuolation of seminiferous tubules in immature mice. Moreover, daily sperm production (DSP) was significantly less in the fetal ASD-exposed mice than in the controls. DSP in the fetal ASD-exposed mice was approximately 10% less than the controls at both 5 and 10 weeks. However, both the histologic changes and the DSP decrease were reversed as the mice matured. These findings suggest that ASD exposure affects both the fetal development and the reproduction of male offspring. In the future, it will be necessary to clarify the onset mechanisms of ASD-induced male fetus death and male reproductive disorders.

  5. Rapidly developing iatrogenic hyponatremia in a child following tonsillectomy.

    Science.gov (United States)

    Taskin, Umit; Binay, Omer; Binay, Cigdem; Yigit, Ozgur

    2012-11-01

    Hyponatremia develops as a result of the inappropriate secretion of antidiuretic hormone. In rare cases, it develops as an iatrogenic complication. For example, acute iatrogenic post-tonsillectomy hyponatremia has been described in children following the infusion of hypo- or isotonic fluid. We report a case of rapidly developing post-tonsillectomy iatrogenic hyponatremia in a 5-year-old girl following an excessive infusion of hypotonic fluid. Her signs and symptoms began with nausea and vomiting and progressed to seizures and coma. We corrected the electrolyte disturbance by infusing a 3% sodium chloride solution until her neurologic manifestations disappeared, at which time her serum sodium concentration had risen back to 135 mEq/L. Otolaryngologists are not generally exposed to much information about hyponatremia, so we must be aware of its associated neurologic signs and symptoms.

  6. Development of Nylon Based FDM Filament for Rapid Tooling Application

    Science.gov (United States)

    Singh, R.; Singh, S.

    2014-04-01

    There has been critical need for development of cost effective nylon based wire to be used as feed stock filament for fused deposition modelling (FDM) machine. But hitherto, very less work has been reported for development of alternate solution of acrylonitrile butadiene styrene (ABS) based wire which is presently used in most of FDM machines. The present research work is focused on development of nylon based wire as an alternative of ABS wire (which is to be used as feedstock filament on FDM) without changing any hardware or software of machine. For the present study aluminium oxide (Al2O3) as additive in different proportion has been used with nylon fibre. Single screw extruder was used for wire preparation and wire thus produced was tested on FDM. Mechanical properties i.e. tensile strength and percentage elongation of finally developed wire have been optimized by Taguchi L9 technique. The work represented major development in reducing cost and time in rapid tooling applications.

  7. The Lay Public’s Understanding and Perception of Dementia in a Developed Asian Nation

    Directory of Open Access Journals (Sweden)

    Wai Jia Tan

    2012-10-01

    Full Text Available Background: Early detection of dementia aims to improve treatment outcomes. However, poor perception and understanding of dementia are significant barriers. We aim to investigate the public’s perception of dementia and identify variables associated with the different profiles of public perception. Methods: A custom-designed questionnaire was used to assess laypersons’ knowledge and perception of dementia during a health fair at a public hospital in Singapore, a developed Asian nation. Out of a sample of 370 subjects, 32 declined to participate (response rate = 91.4%. Latent class analysis (LCA was used to identify meaningful subgroups of subjects from significant associations with multiple indicators of dementia awareness. Multinomial logistic regression was performed exploring variables associated with each of the subgroups derived from LCA. Results: The majority of the study participants were female (66.9%, 65 years or older (71.1%, and ethnic Chinese (88.1%. LCA classified the study participants into 3 subgroups: Class 1 (good knowledge, good attitude, Class 2 (good knowledge, poor attitude, and Class 3 (poor knowledge, poor attitude, in proportions of 14.28, 63.83, and 21.88%, respectively. Compared to other classes, participants with good knowledge and good attitude towards dementia (Class 1 were more likely to know someone with dementia and understand the effects of the disease, be married, live in private housing, receive higher monthly income, and not profess belief in Buddhism, Taoism, or Hinduism. Conclusion: Our results show that the public in Singapore may not be ready for screening initiatives and early dementia diagnosis. Education efforts should be targeted at lower socioeconomic groups, singles, and those of certain oriental religions.

  8. Behavior of bacteriome symbionts during transovarial transmission and development of the Asian citrus psyllid.

    Directory of Open Access Journals (Sweden)

    Hiroki Dan

    Full Text Available The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae is a serious pest worldwide, transmitting Candidatus Liberibacter spp. (Alphaproteobacteria, the causative agents of a devastating citrus disease known as huanglongbing or greening disease. In a symbiotic organ called the bacteriome, D. citri possesses an organelle-like defensive symbiont, Candidatus Profftella armatura (Betaproteobacteria, and a nutritional symbiont, Ca. Carsonella ruddii (Gammaproteobacteria. Drastically reduced symbiont genomes and metabolic complementarity among the symbionts and D. citri indicate their mutually indispensable association. Moreover, horizontal gene transfer between the Profftella and Liberibacter lineages suggests ecological and evolutionary interactions between the bacteriome symbiont and the HLB pathogen. Using fluorescence in situ hybridization, we examined the behavior of Profftella and Carsonella during transovarial transmission and the development of D. citri. In the bacteriomes of sexually-mature female adults, symbionts transformed from an extremely elongated tubular form into spherical or short-rod forms, which migrated toward the ovary. The symbionts then formed mosaic masses, which entered at the posterior pole of the vitellogenic oocytes. After anatrepsis, Carsonella and Profftella migrated to the central and peripheral parts of the mass, respectively. Following the appearance of host nuclei, the mass cellularized, segregating Carsonella and Profftella in the central syncytium and peripheral uninucleate bacteriocytes, respectively. Subsequently, the uninucleate bacteriocytes harboring Profftella assembled at the posterior pole, while the syncytium, containing Carsonella, sat on the anterior side facing the germ band initiating katatrepsis. During dorsal closure, the syncytium was divided into uninuclear bacteriocytes, which surrounded the mass of bacteriocytes containing Profftella. Once fully surrounded, the bacteriocyte mass

  9. Microstructural development of rapid solidification in Al-Si powder

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Feng [Iowa State Univ., Ames, IA (United States)

    1995-09-26

    The microstructure and the gradient of microstructure that forms in rapidly solidificated powder were investigated for different sized particles. High pressure gas atomization solidification process has been used to produce a series of Al-Si alloys powders between 0.2 μm to 150 μm diameter at the eutectic composition (12.6 wt pct Si). This processing technique provides powders of different sizes which solidify under different conditions (i.e. interface velocity and interface undercooling), and thus give different microstructures inside the powders. The large size powder shows dendritic and eutectic microstructures. As the powder size becomes smaller, the predominant morphology changes from eutectic to dendritic to cellular. Microstructures were quantitatively characterized by using optical microscope and SEM techniques. The variation in eutectic spacing within the powders were measured and compared with the theoretical model to obtain interface undercooling, and growth rate during the solidification of a given droplet. Also, nucleation temperature, which controls microstructures in rapidly solidified fine powders, was estimated. A microstructural map which correlates the microstructure with particle size and processing parameters is developed.

  10. Microstructural Development in Al-Si Powder During Rapid Solidification

    Energy Technology Data Exchange (ETDEWEB)

    Genau, Amber Lynn [Iowa State Univ., Ames, IA (United States)

    2004-01-01

    Powder metallurgy has become an increasingly important form of metal processing because of its ability to produce materials with superior mechanical properties. These properties are due in part to the unique and often desirable microstructures which arise as a result of the extreme levels of undercooling achieved, especially in the finest size powder, and the subsequent rapid solidification which occurs. A better understanding of the fundamental processes of nucleation and growth is required to further exploit the potential of rapid solidification processing. Aluminum-silicon, an alloy of significant industrial importance, was chosen as a model for simple eutectic systems displaying an unfaceted/faceted interface and skewed coupled eutectic growth zone, Al-Si powder produced by high pressure gas atomization was studied to determine the relationship between microstructure and alloy composition as a function of powder size and atomization gas. Critical experimental measurements of hypereutectic (Si-rich) compositions were used to determine undercooling and interface velocity, based on the theoretical models which are available. Solidification conditions were analyzed as a function of particle diameter and distance from nucleation site. A revised microstructural map is proposed which allows the prediction of particle morphology based on temperature and composition. It is hoped that this work, by providing enhanced understanding of the processes which govern the development of the solidification morphology of gas atomized powder, will eventually allow for better control of processing conditions so that particle microstructures can be optimized for specific applications.

  11. Rapid Development of Bespoke Unmanned Platforms for Atmospheric Science

    Science.gov (United States)

    Sobester, A.; Johnston, S. J.; Scanlan, J. P.; Hart, E. E.; O'Brien, N. S.

    2012-04-01

    The effective deployment of airborne atmospheric science instruments often hinges on the development cycle time of a suitable platform, one that is capable of delivering them to the desired altitude range for a specified amount of time, along a pre-determined trajectory. This could be driven by the need to respond rapidly to sudden, unexpected events (e.g., volcano eruptions, nuclear fallout, etc.) or simply to accommodate the iterative design and flight test cycle of the instrument developer. A shorter development cycle time would also afford us the ability to quickly adapt the hardware and control logic in response to unexpected results during an experimental campaign. We report on recent developments aimed at meeting this demand. As part of the Atmospheric Science Through Robotic Aircraft (ASTRA) initiative we have investigated the use of rapid prototyping technologies to this end, both on the 'airframe' of the platform itself and on the on-board systems. We show how fast multi-disciplinary design optimization techniques, coupled with computer-controlled additive manufacturing (3D printing) and laser cutting methods and electronic prototyping (using standard, modular, programmable building blocks) can lead to the delivery of a fully customized platform integrating a given instrument in a timescale of the order of ten days. Specific examples include the design and testing of a balloon-launched glider sensorcraft and a stratospheric balloon system. The 'vehicle' for the latter was built on a 3D printer using a copolymer thermoplastic material and fitted with a sacrificial protective 'cage' laser-cut from an open-cell foam. The data logging, tracking, sensor integration and communications services of the platform were constructed using the .net Gadgeteer open source hardware kit. The flight planning and eventual post-flight recovery of the system is enabled by a generic, stochastic trajectory simulation tool, also developed as part of the ASTRA initiative. This

  12. Improved rapid prototyping methodology for MPEG-4 IC development

    Science.gov (United States)

    Tang, Clive K. K.; Moseler, Kathy; Levi, Sami

    1998-12-01

    One important factor in deciding the success of a new consumer product or integrated circuit is minimized time-to- market. A rapid prototyping methodology that encompasses algorithm development in the hardware design phase will have great impact on reducing time-to-market. In this paper, a proven hardware design methodology and a novel top-down design methodology based on Frontier Design's DSP Station tool are described. The proven methodology was used during development of the MC149570 H.261/H.263 video codec manufactured by Motorola. This paper discusses an improvement to this method to create an integrated environment for both system and hardware development, thereby further reducing the time-to-market. The software tool chosen is DSP Station tool by Frontier Design. The rich features of DSP Station tool will be described and then it will be shown how these features may be useful in designing from algorithm to silicon. How this methodology may be used in the development of a new MPEG4 Video Communication ASIC will be outlined. A brief comparison with a popular tool, Signal Processing WorkSystem tool by Cadence, will also be given.

  13. Rapid Response Risk Assessment in New Project Development

    Science.gov (United States)

    Graber, Robert R.

    2010-01-01

    A capability for rapidly performing quantitative risk assessments has been developed by JSC Safety and Mission Assurance for use on project design trade studies early in the project life cycle, i.e., concept development through preliminary design phases. A risk assessment tool set has been developed consisting of interactive and integrated software modules that allow a user/project designer to assess the impact of alternative design or programmatic options on the probability of mission success or other risk metrics. The risk and design trade space includes interactive options for selecting parameters and/or metrics for numerous design characteristics including component reliability characteristics, functional redundancy levels, item or system technology readiness levels, and mission event characteristics. This capability is intended for use on any project or system development with a defined mission, and an example project will used for demonstration and descriptive purposes, e.g., landing a robot on the moon. The effects of various alternative design considerations and their impact of these decisions on mission success (or failure) can be measured in real time on a personal computer. This capability provides a high degree of efficiency for quickly providing information in NASA s evolving risk-based decision environment

  14. Rapid Application Development with OpenStudio: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Weaver, E.; Long, N.; Fleming, K.; Schott, M.; Benne, K.; Hale, E.

    2012-05-01

    This paper presents several case studies of rapidly implemented, audience-specific applications for whole building energy modeling and standards analysis. By tailoring each application to the audience and the task at hand, the required learning curve for new users was greatly reduced. Each case study used OpenStudio, the U.S. Department of Energy's middleware software development kit (SDK). OpenStudio provides an easy interface to the EnergyPlus whole building simulation engine, while extending its capability and providing higher-level functionality such as software interoperability, standards, analysis, and optimization. Each case study is unique in the technology employed to interface with OpenStudio as well as the methods used for user interaction and data presentation. Four case studies are presented.

  15. Polygenic Risk, Rapid Childhood Growth, and the Development of Obesity

    Science.gov (United States)

    Belsky, Daniel W.; Moffitt, Terrie E.; Houts, Renate; Bennett, Gary G.; Biddle, Andrea K.; Blumenthal, James A.; Evans, James P.; Harrington, HonaLee; Sugden, Karen; Williams, Benjamin; Poulton, Richie; Caspi, Avshalom

    2012-01-01

    Objective To test how genomic loci identified in genome-wide association studies influence the development of obesity. Design A 38-year prospective longitudinal study of a representative birth cohort. Setting The Dunedin Multidisciplinary Health and Development Study, Dunedin, New Zealand. Participants One thousand thirty-seven male and female study members. Main Exposures We assessed genetic risk with a multilocus genetic risk score. The genetic risk score was composed of single-nucleotide polymorphisms identified in genome-wide association studies of obesity-related phenotypes. We assessed family history from parent body mass index data collected when study members were 11 years of age. Main Outcome Measures Body mass index growth curves, developmental phenotypes of obesity, and adult obesity outcomes were defined from anthropometric assessments at birth and at 12 subsequent in-person interviews through 38 years of age. Results Individuals with higher genetic risk scores were more likely to be chronically obese in adulthood. Genetic risk first manifested as rapid growth during early childhood. Genetic risk was unrelated to birth weight. After birth, children at higher genetic risk gained weight more rapidly and reached adiposity rebound earlier and at a higher body mass index. In turn, these developmental phenotypes predicted adult obesity, mediating about half the genetic effect on adult obesity risk. Genetic associations with growth and obesity risk were independent of family history, indicating that the genetic risk score could provide novel information to clinicians. Conclusions Genetic variation linked with obesity risk operates, in part, through accelerating growth in the early childhood years after birth. Etiological research and prevention strategies should target early childhood to address the obesity epidemic. PMID:22665028

  16. Research and development of transgenic plants in Malaysia: an example from an Asian developing country.

    Science.gov (United States)

    Hashim, Marzukhi; Osman, Mohamad; Abdullah, Ruslan; Pillai, Vilasini; Abu Bakar, Umi K; Hashim, Habibuddin; Daud, Hassan Mat

    2002-12-01

    In 2000, agriculture contributed 13% to the national gross domestic product of Malaysia. The country of 23 million people has created a competitive program coordinated by the Ministry of Science, Technology and the Environment, research institutions, and universities to undertake biotechnology research in several areas. Intensified research efforts are under way on oil palm, rubber, rice, papaya, and orchids. Although the most progress has been made in rice and papaya, no transgenic crop is ready for field trials. Nonetheless, preliminary steps have been taken to prepare for the trials, and detailed testing protocols are being developed.

  17. Central Asian Republic Info

    Data.gov (United States)

    US Agency for International Development — CAR Info is designed and managed by the Central Asian Republic Mission to fill in the knowledge and reporting gaps in existing agency systems for that Mission. It...

  18. Process-oriented evaluation of an international faculty development program for Asian developing countries: a qualitative study.

    Science.gov (United States)

    Kim, Do-Hwan; Lee, Jong-Hyuk; Park, Jean; Shin, Jwa-Seop

    2017-12-21

    Non-English-speaking developing countries in Southeast Asia have been provided only limited opportunities for faculty development in the education of health professions. Although there exist a few programs that have been shown to be effective, they are frequently presented with few explanations on how and why the programs work due to their outcome-oriented nature. This study explores the process of the Lee Jong-Wook Fellowship for Health Professional Education, an international faculty development program designed for capacity building of educators of health professions in Southeast Asian developing countries. Fellows were from Cambodia, Myanmar, and Laos. Qualitative data were collected from two types of semi-structured interviews - group and individual. Thematic analysis was conducted to explore the factors related to the effectiveness of the program, framed by four components of faculty development, which included context, facilitators, program, and participants. From the thematic analysis, the authors identified a total of 12 themes in the four components of faculty development. In the context domain, the resource-poor setting, a culture that puts emphasis on hierarchy and seniority, and educational environment depending on individual commitment rather than broad consensus emerged as key factors. In the facilitators domain, their teaching methods and materials, mutual understanding between teacher and learner, and collaboration between facilitators mainly influenced the learning during the fellowship. In the program domain, the key advantages of the fellowship program were its applicability to the workplace of the fellows and enough allowed time for practice and reflection. Finally, in the participants domain, Fellows valued their heterogeneity of composition and recognized cognitive as well as non-cognitive attributes of the participants as essential. This process-oriented evaluation reveals the diverse factors that contributed to achieving the intended

  19. The international implications of the Chinese model of development in the Global South: Asian Consensus as a network power

    Directory of Open Access Journals (Sweden)

    Javier Vadell

    2014-01-01

    Full Text Available This paper analyzes People's Republic of China (PRC economic and political ascendance in the 21st century focusing on the evolution of the sui generis economic development model and its significances of the evolution of relationship between China and the developing countries in the peripheral "Global South." The objective of this article is to analyze the relationship between China and the Global South (Africa and South America in the 21st century, characterized as a new Center-periphery global network power based on trade and investment that we call as "Asian Consensus."

  20. Cerebellum of the Premature Infant: Rapidly Developing, Vulnerable, Clinically Important

    Science.gov (United States)

    Volpe, Joseph J.

    2009-01-01

    Brain abnormality in surviving premature infants is associated with an enormous amount of neurodevelopmental disability, manifested principally by cognitive, behavioral, attentional, and socialization deficits, most commonly with only relatively modest motor deficits. The most recognized contributing neuropathology is cerebral white matter injury. The thesis of this review is that acquired cerebellar abnormality is a relatively less recognized but likely important cause of neurodevelopmental disability in small premature infants. The cerebellar disease may be primarily destructive (eg, hemorrhage, infarction) or primarily underdevelopment. The latter appears to be especially common and relates to a particular vulnerability of the cerebellum of the small premature infant. Central to this vulnerability are the extraordinarily rapid and complex developmental events occurring in the cerebellum. The disturbance of development can be caused either by direct adverse effects on the cerebellum, especially the distinctive transient external granular layer, or by indirect remote trans-synaptic effects. This review describes the fascinating details of cerebellar development, with an emphasis on events in the premature period, the major types of cerebellar abnormality acquired during the premature period, their likely mechanisms of occurrence, and new insights into the relation of cerebellar disease in early life to subsequent cognitive/behavioral/attentional/socialization deficits. PMID:19745085

  1. RAPIDE 0.0 RHIC Accelerator Physics Intrepid Development Environment

    Energy Technology Data Exchange (ETDEWEB)

    Satogata, T. [Brookhaven National Lab. (BNL), Upton, NY (United States); Saltmarsh, C. [Brookhaven National Lab. (BNL), Upton, NY (United States); Peggs, S. [Brookhaven National Lab. (BNL), Upton, NY (United States)

    1993-08-01

    This document is a guide to the common environmental features of computing in (and around) the RHIC Accelerator Physics.sectio on the 'zoo' cluster of UNJX workstations, in RAPIDE, the RHIC Accelerator Physics Intrepid Development Environment It is hoped tliat later revisions of this document will approach a more professional 'style guide', beyond the convenient collection of pointers and hints presented here. RAP does two kinds of computing, "controls" and "general", addressed in sections 2 and 3 of this document For general computing, efficient system administration requires cooperation in using a common environment There is a much stronger need to define - and adhere to - a commonly agreed set of styles (or rules) in developing controls software. Right now, these rules have been set "de facto". Future improvements to the controls environment, particularly in response to the opinions of users, depends on broad knowledge of what the rules are. There are environmental issues that are basic to both controls and general computing, and that are so fundamental that they are (almost) unarguable. They are described immediately below, in the next section.

  2. The development of simple survival prediction models for blunt trauma victims treated at Asian emergency centers.

    Science.gov (United States)

    Kimura, Akio; Nakahara, Shinji; Chadbunchachai, Witaya

    2012-02-02

    For real-time assessment of the probability of survival (Ps) of blunt trauma victims at emergency centers, this study aimed to establish regression models for estimating Ps using simplified coefficients. The data of 10,210 blunt trauma patients not missing both the binary outcome data about survival and the data necessary for Ps calculation by The Trauma and Injury Severity Score (TRISS) method were extracted from the Japan Trauma Data Bank (2004-2007) and analyzed. Half (5,113) of the data was allocated to a derivation data set, with the other half (5,097) allocated to a validation data set. The data of 6,407 blunt trauma victims from the trauma registry of Khon Kaen Regional Hospital in Thailand were analyzed for validation. The logistic regression models included age, the Injury Severity Score (ISS), the Glasgow Coma Scale score (GCS), systolic blood pressure (SBP), respiratory rate (RR), and their coded values (cAGE, 0-1; cISS, 0-4; cSBP, 0-4; cGCS, 0-4; cRR, 0-4) as predictor variables. The coefficients were simplified by rounding off after the decimal point or choosing 0.5 if the coefficients varied across 0.5. The area under the receiver-operating characteristic curve (AUROCC) was calculated for each model to measure discriminant ability. A group of formulas (log (Ps/1-Ps) = logit (Ps) = -9 + cISS - cAGE + cSBP + cGCS + cRR/2, where -9 becomes -7 if the predictor variable of cRR or cISS is missing) was developed. Using these formulas, the AUROCCs were between 0.950 and 0.964. When these models were applied to the Khon Kean data, their AUROCCs were greater than 0.91. These equations allow physicians to perform real-time assessments of survival by easy mental calculations at Asian emergency centers, which are overcrowded with blunt injury victims of traffic accidents. © 2012 Kimura et al; licensee BioMed Central Ltd.

  3. The development of simple survival prediction models for blunt trauma victims treated at Asian emergency centers

    Directory of Open Access Journals (Sweden)

    Kimura Akio

    2012-02-01

    Full Text Available Abstract Background For real-time assessment of the probability of survival (Ps of blunt trauma victims at emergency centers, this study aimed to establish regression models for estimating Ps using simplified coefficients. Methods The data of 10,210 blunt trauma patients not missing both the binary outcome data about survival and the data necessary for Ps calculation by The Trauma and Injury Severity Score (TRISS method were extracted from the Japan Trauma Data Bank (2004-2007 and analyzed. Half (5,113 of the data was allocated to a derivation data set, with the other half (5,097 allocated to a validation data set. The data of 6,407 blunt trauma victims from the trauma registry of Khon Kaen Regional Hospital in Thailand were analyzed for validation. The logistic regression models included age, the Injury Severity Score (ISS, the Glasgow Coma Scale score (GCS, systolic blood pressure (SBP, respiratory rate (RR, and their coded values (cAGE, 0-1; cISS, 0-4; cSBP, 0-4; cGCS, 0-4; cRR, 0-4 as predictor variables. The coefficients were simplified by rounding off after the decimal point or choosing 0.5 if the coefficients varied across 0.5. The area under the receiver-operating characteristic curve (AUROCC was calculated for each model to measure discriminant ability. Results A group of formulas (log (Ps/1-Ps = logit (Ps = -9 + cISS - cAGE + cSBP + cGCS + cRR/2, where -9 becomes -7 if the predictor variable of cRR or cISS is missing was developed. Using these formulas, the AUROCCs were between 0.950 and 0.964. When these models were applied to the Khon Kean data, their AUROCCs were greater than 0.91. Conclusion: These equations allow physicians to perform real-time assessments of survival by easy mental calculations at Asian emergency centers, which are overcrowded with blunt injury victims of traffic accidents.

  4. Aniline Is Rapidly Converted Into Paracetamol Impairing Male Reproductive Development.

    Science.gov (United States)

    Holm, Jacob Bak; Chalmey, Clementine; Modick, Hendrik; Jensen, Lars Skovgaard; Dierkes, Georg; Weiss, Tobias; Jensen, Benjamin Anderschou Holbech; Nørregård, Mette Marie; Borkowski, Kamil; Styrishave, Bjarne; Martin Koch, Holger; Mazaud-Guittot, Severine; Jegou, Bernard; Kristiansen, Karsten; Kristensen, David Møbjerg

    2015-11-01

    Industrial use of aniline is increasing worldwide with production estimated to surpass 5.6 million metric tons in 2016. Exposure to aniline occurs via air, diet, and water augmenting the risk of exposing a large number of individuals. Early observations suggest that aniline is metabolized to paracetamol/acetaminophen, likely explaining the omnipresence of low concentrations of paracetamol in European populations. This is of concern as recent studies implicate paracetamol as a disrupter of reproduction. Here, we show through steroidogenic profiling that exposure to aniline led to increased levels of the Δ4 steroids, suggesting that the activity of CYP21 was decreased. By contrast, paracetamol decreased levels of androgens likely through inhibition of CYP17A1 activity. We confirm that aniline in vivo is rapidly converted to paracetamol by the liver. Intrauterine exposure to aniline and paracetamol in environmental and pharmaceutical relevant doses resulted in shortening of the anogenital distance in mice, a sensitive marker of fetal androgen levels that in humans is associated with reproductive malformations and later life reproductive disorders. In conclusion, our results provide evidence for a scenario where aniline, through its conversion into antiandrogenic paracetamol, impairs male reproductive development. © The Author 2015. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  5. Development of Multiple Big Data Analytics Platforms with Rapid Response

    Directory of Open Access Journals (Sweden)

    Bao Rong Chang

    2017-01-01

    Full Text Available The crucial problem of the integration of multiple platforms is how to adapt for their own computing features so as to execute the assignments most efficiently and gain the best outcome. This paper introduced the new approaches to big data platform, RHhadoop and SparkR, and integrated them to form a high-performance big data analytics with multiple platforms as part of business intelligence (BI to carry out rapid data retrieval and analytics with R programming. This paper aims to develop the optimization for job scheduling using MSHEFT algorithm and implement the optimized platform selection based on computing features for improving the system throughput significantly. In addition, users would simply give R commands rather than run Java or Scala program to perform the data retrieval and analytics in the proposed platforms. As a result, according to performance index calculated for various methods, although the optimized platform selection can reduce the execution time for the data retrieval and analytics significantly, furthermore scheduling optimization definitely increases the system efficiency a lot.

  6. Drosophila provides rapid modeling of renal development, function, and disease

    Science.gov (United States)

    Romero, Michael F.

    2010-01-01

    The evolution of specialized excretory cells is a cornerstone of the metazoan radiation, and the basic tasks performed by Drosophila and human renal systems are similar. The development of the Drosophila renal (Malpighian) tubule is a classic example of branched tubular morphogenesis, allowing study of mesenchymal-to-epithelial transitions, stem cell-mediated regeneration, and the evolution of a glomerular kidney. Tubule function employs conserved transport proteins, such as the Na+, K+-ATPase and V-ATPase, aquaporins, inward rectifier K+ channels, and organic solute transporters, regulated by cAMP, cGMP, nitric oxide, and calcium. In addition to generation and selective reabsorption of primary urine, the tubule plays roles in metabolism and excretion of xenobiotics, and in innate immunity. The gene expression resource FlyAtlas.org shows that the tubule is an ideal tissue for the modeling of renal diseases, such as nephrolithiasis and Bartter syndrome, or for inborn errors of metabolism. Studies are assisted by uniquely powerful genetic and transgenic resources, the widespread availability of mutant stocks, and low-cost, rapid deployment of new transgenics to allow manipulation of renal function in an organotypic context. PMID:20926630

  7. Endosulfan and flutamide impair testicular development in the juvenile Asian catfish, Clarias batrachus

    Energy Technology Data Exchange (ETDEWEB)

    Rajakumar, A.; Singh, R.; Chakrabarty, S.; Murugananthkumar, R.; Laldinsangi, C.; Prathibha, Y.; Sudhakumari, C.C.; Dutta-Gupta, A. [Department of Animal Sciences, School of Life Sciences-Centre for Advanced Studies, University of Hyderabad, P. O. Central University, Hyderabad 500046, Andhra Pradesh (India); Senthilkumaran, B., E-mail: bsksl@uohyd.ernet.in [Department of Animal Sciences, School of Life Sciences-Centre for Advanced Studies, University of Hyderabad, P. O. Central University, Hyderabad 500046, Andhra Pradesh (India)

    2012-04-15

    Highlights: Black-Right-Pointing-Pointer Endosulfan and/or flutamide retard(s) testicular differentiation in juvenile fish. Black-Right-Pointing-Pointer Endosulfan and/or flutamide target(s) cfGnRH-Tph2 axis, either directly or indirectly. Black-Right-Pointing-Pointer Effects of endosulfan and/or flutamide on plasma androgen levels. Black-Right-Pointing-Pointer Compared changes in testis-related transcripts with testis germ cell distribution. Black-Right-Pointing-Pointer Our study on endosulfan endorse its prompt and complete phase-out/ban. - Abstract: Endosulfan and flutamide, a widely used pesticide and a prostate cancer/infertility drug, respectively, have an increased risk of causing endocrine disruption if they reach water bodies. Though many studies are available on neurotoxicity/bioaccumulation of endosulfan and receptor antagonism of flutamide, only little is known about their impact on testicular steroidogenesis at molecular level. Sex steroids play an important role in sex differentiation of lower vertebrates including fishes. Hence, a small change in their levels caused by endocrine disruptors affects the gonadal development of aquatic vertebrates significantly. The aim of this study was to evaluate the effects of endosulfan and flutamide on testis-related transcription factor and steroidogenic enzyme genes with a comparison on the levels of androgens during critical period of catfish testicular development. We also analyzed the correlation between the above-mentioned genes and catfish gonadotropin-releasing hormone (cfGnRH)-tryptophan hydroxylase2 (tph2). The Asian catfish, Clarias batrachus males at 50 days post hatch (dph) were exposed to very low dose of endosulfan (2.5 {mu}g/L) and flutamide (33 {mu}g/L), alone and in combination for 50 days. The doses used in this study were far less than those used in the previous studies of flutamide and reported levels of endosulfan in surface water and sediments. Sampling was done at end of the treatments (100

  8. Rapid Cycle Amine (RCA) 3.0 System Development

    Science.gov (United States)

    Chullen, Cinda; Campbell, Colin; Papale, William; Hawes, Kevin; Wichowski, Robert

    2015-01-01

    The Rapid Cycle Amine (RCA) 3.0 system is currently under development by NASA, the Lyndon B. Johnson Space Center (JSC) in conjunction with United Technologies Corporation Aerospace Systems (UTAS). The RCA technology is a new carbon dioxide (CO2) and humidity removal system that has been baselined for the Advanced Extravehicular Mobility Unit (AEMU) Portable Life Support System. The evolution of the RCA development has progressed through several iterations of technology readiness levels including RCA 1.0, RCA 2.0, and RCA 3.0 test articles. The RCA is an advancement over currently technologies due to its unique regeneration capability. The RCA is capable of simultaneously removing CO2 and humidity from an influent air steam and subsequent regeneration when exposed to a vacuum source. The RCA technology uses two solid amine sorbent beds in an alternating fashion to adsorb CO2 and water (uptake mode) and desorb CO2 and water (regeneration mode) at the same time. The two beds operate in an efficient manner so that while one bed is in the uptake mode, the other is in the regeneration mode, thus continuously providing an on-service sorbent bed by which CO2 and humidity may be removed. The RCA 2.0 and 3.0 test articles were designed with a novel valve assembly which allows for switching between uptake and regeneration modes with only one moving part while minimizing gas volume losses to the vacuum source by means of an internal pressure equalization step during actuation. The RCA technology also is low power, small, and has performed extremely well in all development testing thus far. A final design was selected for the RCA 3.0, fabricated, assembled, and performance tested in 2014 with delivery to NASAJSC in January 2015. This paper will provide an overview on the RCA 3.0 system design and results of pre-delivery testing with references to the development of RCA 1.0 and RCA 2.0.

  9. China as leading Asian giant and her indispensability within BRICS ...

    African Journals Online (AJOL)

    China is unequivocally the “Leading Giant” of Brics Group. The rapid economic growth and expansion of influence throughout the whole Asian region inspires and accelerates the other Brics economies development. The countries are believed to “catch-up” Chinese growth- strategies and technologies. Foreign Direct ...

  10. [Rapidly developing vertebral eosinophilic granuloma. Apropos of a case].

    Science.gov (United States)

    Laburthe-Tolra, Y; Boutillier, J B; Chome, J

    1983-04-01

    Radiographs taken at an interval of one week showed collapse of the twelfth dorsal vertebra, very suggestive of an eosinophilic granuloma. A biopsy performed during surgery, because of the rapid progression of the lesion, was able to confirm the diagnosis.

  11. Development of a Forecasting and Data Assimilation System for Asian Dust in the Japan Meteorological Agency (JMA)

    Science.gov (United States)

    Yumimoto, K.; Tanaka, T. Y.; Ogi, A.; Sekiyama, T. T.; Maki, T.; Murakami, H.; Kikuchi, M.; Nagao, T. M.

    2015-12-01

    Mineral dust, a major aerosol during springtime in East Asia, impacts various aspects including social activity, human health, climate and the ocean ecosystem. To mitigate the damage of severe dust storms, it is crucial to develop a forecasting and early warning system for Asian dust. Since 2007, the World Meteorological Organization (WMO) has taken the lead with 40 international partners to develop a Sand and Dust Storm Warning Advisory and Assessment System (SDS-WAS). The Japan Meteorological Agency (JMA) launched a numerical forecasting system for Asian dust in 2004, and completed a major renovation of the system in November 2014. In the renovation, we replaced a general circulation model (the JMA98 GCM) and dust emission scheme (based on wind velocity at 10 m) with new ones (the GSMUV GCM and a friction velocity based emission scheme). A 5-year validation exhibits that the renovation achieves better forecasting score (especially in short range forecast). Our group has resolution improvement (up to ~40 km) and implementation of data assimilation with satellite observations in the upcoming updates. A feasibility study on involving observations from Himawari-8 (JMA's new geostationary meteorological satellite) into the system is also conducted for better forecasting skill and toward robust early warning.

  12. Asian Ginseng

    Science.gov (United States)

    ... senticosus) is not related to true ginseng. In traditional Chinese medicine, Asian ginseng was used as a ... recommend against its use by infants, children, and women who are pregnant or breastfeeding. The most common ...

  13. Late Miocene?Pliocene development of Asian aridification as recorded in the Red-Earth Formation in northern China

    Science.gov (United States)

    Guo, Zhengtang; Peng, Shuzhen; Hao, Qingzhen; Biscaye, Pierre E.; An, Zhisheng; Liu, Tungsheng

    2004-07-01

    In northern China, the Late Miocene-Pliocene Hipparion Red-Earth Formation in the eastern Loess Plateau fills a gap of climate records between the well-known loess-soil sequences of the last 2.6 Ma and the Miocene loess-soil sequences from the western Loess Plateau. Earlier studies on type sections indicate that a major part of the Red-Earth Formation is also of wind-blown origin, covering the period from ˜7-8 to ˜2.6 Ma BP. Because the eolian dust deposited in the region originated from the Asian desert lands, the Red-Earth must contain a record of the aridification history of the Asian interior. In this study, the Xifeng type section in the eastern Loess Plateau is studied to assess the development of Asian aridification during Late Miocene-Pliocene time. Eolian dust deposition continued at the central Loess Plateau since ˜6.2 Ma BP, indicating that sizable desert lands in the interior of Asia and the Asian winter monsoon must have been constantly maintained during Late Miocene-Pliocene time, and were able to provide a significant amount of eolian dust. The aridity in the source areas was stronger from ˜6.2 to ˜5 Ma BP and weaker from ˜5 to ˜3.6 Ma BP. Two major aridification steps are observed at ˜3.6 and ˜2.6 Ma BP, respectively. The intensification of eolian deposition at ˜3.6 Ma BP is synchronous with a suggested uplift of portions of the Tibetan Plateau. The general aridification history is also highly consistent with the ongoing high-latitude cooling and the consequent expansion of Arctic sea-ice/ice sheets during this interval. Our results therefore suggest that both Tibetan uplift and ice-building processes in the northern hemisphere were two prominent driving forces behind the long-term desertification in the interior of Asia during Late Miocene and Pliocene time.

  14. Diabetes in Asians

    Directory of Open Access Journals (Sweden)

    Eun-Jung Rhee

    2015-09-01

    Full Text Available The prevalence of diabetes is increasing globally, particularly in Asia. According to the 2013 Diabetes Atlas, an estimated 366 million people are affected by diabetes worldwide; 36% of those affected live in the Western Pacific region, with a significant proportion in East Asia. The reasons for this marked increase in the prevalence of diabetes can be extrapolated from several distinct features of the Asian region. First, the two most populated countries, China and India, are located in Asia. Second, Asians have experienced extremely rapid economic growth, including rapid changes in dietary patterns, during the past decades. As a result, Asians tend to have more visceral fat within the same body mass index range compared with Westerners. In addition, increased insulin resistance relative to reduced insulin secretory function is another important feature of Asian individuals with diabetes. Young age of disease onset is also a distinctive characteristic of these patients. Moreover, changing dietary patterns, such as increased consumption of white rice and processed red meat, contributes to the deteriorated lifestyle of this region. Recent studies suggest a distinctive responsiveness to novel anti-diabetic agents in Asia; however, further research and efforts to reverse the increasing prevalence of diabetes are needed worldwide.

  15. Rapid gas development in the Fayetteville shale basin, Arkansas

    Science.gov (United States)

    Advances in drilling and extraction of natural gas have resulted in rapid expansion of wells in shale basins. The rate of gas well installation in the Fayetteville shale is 774 wells a year since 2005 with thousands more planned. The Fayetteville shale covers 23,000 km2 although ...

  16. Development of a potential lignocellulolytic resource for rapid ...

    African Journals Online (AJOL)

    This study was conducted at the Laboratory of Food Crops and Floriculture, Institute of Tropical Agriculture, Universiti Putra Malaysia to characterize the potential lignocellulolytic bacteria for rapid bio-bioconversion of rice straw. Fifty isolates of bacteria were isolated from several in-situ and in-vitro sources. Isolates B3, B13 ...

  17. Developing Collective Learning Extension for Rapidly Evolving Information System Courses

    Science.gov (United States)

    Agarwal, Nitin; Ahmed, Faysal

    2017-01-01

    Due to rapidly evolving Information System (IS) technologies, instructors find themselves stuck in the constant game of catching up. On the same hand students find their skills obsolete almost as soon as they graduate. As part of IS curriculum and education, we need to emphasize more on teaching the students "how to learn" while keeping…

  18. Development of rapid phenotypic system for the identification of ...

    Indian Academy of Sciences (India)

    Rapid and accurate identification of bacterial pathogens is a fundamental goal of clinical microbiology. The diagnosis and surveillance of diseases is dependent, to a great extent, on laboratory services, which cannot function without effective reliable reagents and diagnostics. Despite the advancement in microbiology ...

  19. Successes and challenges of north–south partnerships – key lessons from the African/Asian Regional Capacity Development projects

    Science.gov (United States)

    Färnman, Rosanna; Diwan, Vishal; Zwarenstein, Merrick; Atkins, Salla

    2016-01-01

    Introduction Increasing efforts are being made globally on capacity building. North–south research partnerships have contributed significantly to enhancing the research capacity in low- and middle-income countries (LMICs) over the past few decades; however, a lack of skilled researchers to inform health policy development persists, particularly in LMICs. The EU FP7 funded African/Asian Regional Capacity Development (ARCADE) projects were multi-partner consortia aimed to develop a new generation of highly trained researchers from universities across the globe, focusing on global health-related subjects: health systems and services research and research on social determinants of health. This article aims to outline the successes, challenges and lessons learned from the life course of the projects, focusing on the key outputs and experiences of developing and implementing these two projects together with sub-Saharan African, Asian and European institution partners. Design Sixteen participants from 12 partner institutions were interviewed. The data were analysed using thematic content analysis, which resulted in four themes and three sub-categories. These data were complemented by a review of project reports. Results The results indicated that the ARCADE projects have been successful in developing and delivering courses, and have reached over 920 postgraduate students. Some partners thought the north–south and south–south partnerships that evolved during the project were the main achievement. However, others found there to be a ‘north–south divide’ in certain aspects. Challenges included technical constraints and quality assurance. Additionally, adapting new teaching and learning methods into current university systems was challenging, combined with not being able to award students with credits for their degrees. Conclusion The ARCADE projects were introduced as an innovative and ambitious project idea, although not designed appropriately for all partner

  20. Successes and challenges of north-south partnerships - key lessons from the African/Asian Regional Capacity Development projects.

    Science.gov (United States)

    Färnman, Rosanna; Diwan, Vishal; Zwarenstein, Merrick; Atkins, Salla

    2016-01-01

    Increasing efforts are being made globally on capacity building. North-south research partnerships have contributed significantly to enhancing the research capacity in low- and middle-income countries (LMICs) over the past few decades; however, a lack of skilled researchers to inform health policy development persists, particularly in LMICs. The EU FP7 funded African/Asian Regional Capacity Development (ARCADE) projects were multi-partner consortia aimed to develop a new generation of highly trained researchers from universities across the globe, focusing on global health-related subjects: health systems and services research and research on social determinants of health. This article aims to outline the successes, challenges and lessons learned from the life course of the projects, focusing on the key outputs and experiences of developing and implementing these two projects together with sub-Saharan African, Asian and European institution partners. Sixteen participants from 12 partner institutions were interviewed. The data were analysed using thematic content analysis, which resulted in four themes and three sub-categories. These data were complemented by a review of project reports. The results indicated that the ARCADE projects have been successful in developing and delivering courses, and have reached over 920 postgraduate students. Some partners thought the north-south and south-south partnerships that evolved during the project were the main achievement. However, others found there to be a 'north-south divide' in certain aspects. Challenges included technical constraints and quality assurance. Additionally, adapting new teaching and learning methods into current university systems was challenging, combined with not being able to award students with credits for their degrees. The ARCADE projects were introduced as an innovative and ambitious project idea, although not designed appropriately for all partner institutions. Some challenges were underestimated

  1. Developing a Rapid Algorithm to Enable Rapid Characterization of Alginate Microcapsules

    OpenAIRE

    Chan, Ka Hei; Krishnan, Rahul; Alexander, Michael; Lakey, Jonathan R. T.

    2017-01-01

    The islets of Langerhans are endocrine tissue clusters that secrete hormones that regulate the body's glucose, carbohydrate, and fat metabolism, the most important of which is insulin, a hormone secreted by β-cells within the islets. In certain instances, a person's own immune system attacks and destroys them, leading to the development of type 1 diabetes (T1D), a life-long condition that needs daily insulin administration to maintain health and prolong survival. Islet transplantation is a su...

  2. Developing a Rapid Algorithm to Enable Rapid Characterization of Alginate Microcapsules.

    Science.gov (United States)

    Chan, Ka Hei; Krishnan, Rahul; Alexander, Michael; Lakey, Jonathan R T

    2017-05-09

    The islets of Langerhans are endocrine tissue clusters that secrete hormones that regulate the body's glucose, carbohydrate, and fat metabolism, the most important of which is insulin, a hormone secreted by β-cells within the islets. In certain instances, a person's own immune system attacks and destroys them, leading to the development of type 1 diabetes (T1D), a life-long condition that needs daily insulin administration to maintain health and prolong survival. Islet transplantation is a surgical procedure that has demonstrated the ability to normalize blood sugar levels for up to a few years, but the need for chronic immunosuppression relegates it to a last resort that is often only used sparingly and in seriously ill patients. Islet microencapsulation is a biomedical innovation designed to protect islets from the immune system by coating them with a biocompatible polymer, and this new technology has demonstrated various degrees of success in small- and large-animal studies. This success is significantly impacted by microcapsule morphology and encapsulation efficiency. Since hundreds of thousands of microcapsules are generated during the process, characterization of encapsulated islets without the help of some degree of automation would be difficult, time-consuming, and error prone due to inherent observer bias. We have developed an image analysis algorithm that can analyze hundreds of microencapsulated islets and characterize their size, shape, circularity, and distortion with minimal observer bias. This algorithm can be easily adapted to similar nano- or microencapsulation technologies to implement stricter quality control and improve biomaterial device design and success.

  3. Developing a Rapid Algorithm to Enable Rapid Characterization of Alginate Microcapsules

    Science.gov (United States)

    Chan, Ka Hei; Krishnan, Rahul; Alexander, Michael; Lakey, Jonathan R. T.

    2017-01-01

    The islets of Langerhans are endocrine tissue clusters that secrete hormones that regulate the body's glucose, carbohydrate, and fat metabolism, the most important of which is insulin, a hormone secreted by β-cells within the islets. In certain instances, a person's own immune system attacks and destroys them, leading to the development of type 1 diabetes (T1D), a life-long condition that needs daily insulin administration to maintain health and prolong survival. Islet transplantation is a surgical procedure that has demonstrated the ability to normalize blood sugar levels for up to a few years, but the need for chronic immunosuppression relegates it to a last resort that is often only used sparingly and in seriously ill patients. Islet microencapsulation is a biomedical innovation designed to protect islets from the immune system by coating them with a biocompatible polymer, and this new technology has demonstrated various degrees of success in small- and large-animal studies. This success is significantly impacted by microcapsule morphology and encapsulation efficiency. Since hundreds of thousands of microcapsules are generated during the process, characterization of encapsulated islets without the help of some degree of automation would be difficult, time-consuming, and error prone due to inherent observer bias. We have developed an image analysis algorithm that can analyze hundreds of microencapsulated islets and characterize their size, shape, circularity, and distortion with minimal observer bias. This algorithm can be easily adapted to similar nano- or microencapsulation technologies to implement stricter quality control and improve biomaterial device design and success. PMID:27729095

  4. Talisman--rapid application development for the grid.

    Science.gov (United States)

    Oinn, Thomas M

    2003-01-01

    In order to make use of the emerging grid and network services offered by various institutes and mandated by many current research projects, some kind of user accessible client is required. In contrast with attempts to build generic workbenches, Talisman is designed to allow a bioinformatics expert to rapidly build custom applications, immediately visible using standard web technology, for users who wish to concentrate on the biology of their problem rather than the informatics aspects. As a component of the MyGrid project, it is intended to allow access to arbitrary resources, including but not limited to relational, object and flat file data sources, analysis programs and grid based storage, tracking and distributed annotation systems.

  5. The Development of Rapidly Solidified Magnesium – Copper Ribbons

    Directory of Open Access Journals (Sweden)

    Pastuszak M.

    2016-06-01

    Full Text Available The aim of the present work was to plan and carry out an experiment consisting of amorphization of industrial magnesium alloy WE 43 (Mg - 4 Y - 3 RE - 0.5 Zr modified by the copper addition. Investigated alloy modified with 20% of copper was rapidly quenched with the use of melt spinning technique. The effects of cooling rate on the structure and properties of the obtained material were extensively analyzed. The structure and phase analysis of samples were examined using X-ray diffraction method (XRD while the thermal stability of the samples was determined by differential scanning calorimetry (DSC. Microstructure observations were also conducted. The microhardness tests (HV0.02 and corrosion resistance tests were carried out to investigate the properties of the material. Corrosion resistance measurements were held using a typical three-electrode system. As the result of the research, the effect of cooling rate on microstructure and properties of investigated alloy was determined.

  6. Anticipating Central Asian Water Stress: Variation in River Flow Dependency on Melt Waters from Alpine to Plains in the Remote Tien Shan Range, Kyrgyzstan Using a Rapid Hydro Assessment Methodology

    Science.gov (United States)

    Hill, A. F.; Wilson, A. M.; Williams, M. W.

    2016-12-01

    The future of mountain water resources in High Asia is of high interest to water managers, development organizations and policy makers given large populations downstream reliant on snow and ice sourced river flow. Together with historical and cultural divides among ex-Soviet republics, a lack of central water management following the Soviet break-up has led to water stress as trans-boundary waters weave through and along borders. New upstream hydropower development, a thirsty downstream agricultural sector and a shrinking Aral Sea has led to increasing tension in the region. Despite these pressures and in contrast to eastern High Asia's Himalayan basins (Ganges, Brahmaputra), little attention has been given to western High Asia draining the Pamir and Tien Shan ranges (Syr Darya and Amu Darya basins) to better understand the hydrology of this vast and remote area. Difficult access and challenging terrain exacerbate challenges to working in this remote mountain region. As part of the Contributions to High Asia Runoff from Ice and Snow (CHARIS) project, we asked how does river flow source water composition change over an alpine-to-plains domain of Kyrgyzstan's Naryn River in the Syr Darya basin? In addition, what may the future hold for river flow in Central Asia given the differing responses of snow and ice to climate changes? Utilizing a Rapid Hydrologic Assessment methodology including a suite of pre-field mapping techniques we collected in situ water chemistry data at targeted, remote mountain sites over 450km of the Naryn River over an elevation gradient from glacial headwaters to the lower lying areas - places where people, hydropower and agriculture utilize water. Chemical and isotope tracers were used to separate stream flow to understand relative dependency on melt waters as the river moves downstream from glaciers and snow covered areas. This case study demonstrates a technique to acquire field data over large scales in remote regions that facilitates

  7. Education and Training for Development in East Asia: The Political Economy of Skill Formation in East Asian Newly Industrialised Economies. ESRC Pacific Asia Programme [Series].

    Science.gov (United States)

    Ashton, David; Green, Francis; James, Donna; Sung, Johnny

    This book provides a detailed analysis of the development of education and training systems in Asia and the relationship with the process of economic growth. Focus is on four impoverished agrarian economies--Hong Kong, Singapore, South Korea, and Taiwan--that were transformed in little more than a generation into East Asian "tigers":…

  8. The Career Development of Recently Qualified British South Asian Women Teachers: "Everybody's the Same. I Don't Feel My Ethnicity Is an Issue at All."

    Science.gov (United States)

    Butt, Graham; MacKenzie, Lin; Manning, Russell

    2012-01-01

    This article reports on the final year of a four year research project into the influences on British South Asian women's choice of teaching as a career and on their subsequent career development. The research cohort was interviewed during their initial teacher training year on a PGCE (Post Graduate Certificate of Education) course in the English…

  9. Building Blocks for the Rapid Development of Parallel Simulations Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Scientists need to be able to quickly develop and run parallel simulations without paying the high price of writing low-level message passing codes using compiled...

  10. Kivy framework for rapid development of mobile applications

    OpenAIRE

    Brajar, Gregor

    2016-01-01

    There are more and more tools and libraries, offering multi-platform application development, on the market. This is due increased number of mobile operating systems in the last couple of years. The goal of this thesis is to get familiar with Kivy architecture, which provides support for desktop and mobile application development. We compared the mentioned tool with competing tools called PhoneGap and Titanium. Kivy is a library for Python programming language. All of the functions of the ...

  11. Successes and challenges of north–south partnerships – key lessons from the African/Asian Regional Capacity Development projects

    Directory of Open Access Journals (Sweden)

    Rosanna Färnman

    2016-10-01

    Full Text Available Introduction: Increasing efforts are being made globally on capacity building. North–south research partnerships have contributed significantly to enhancing the research capacity in low- and middle-income countries (LMICs over the past few decades; however, a lack of skilled researchers to inform health policy development persists, particularly in LMICs. The EU FP7 funded African/Asian Regional Capacity Development (ARCADE projects were multi-partner consortia aimed to develop a new generation of highly trained researchers from universities across the globe, focusing on global health-related subjects: health systems and services research and research on social determinants of health. This article aims to outline the successes, challenges and lessons learned from the life course of the projects, focusing on the key outputs and experiences of developing and implementing these two projects together with sub-Saharan African, Asian and European institution partners. Design: Sixteen participants from 12 partner institutions were interviewed. The data were analysed using thematic content analysis, which resulted in four themes and three sub-categories. These data were complemented by a review of project reports. Results: The results indicated that the ARCADE projects have been successful in developing and delivering courses, and have reached over 920 postgraduate students. Some partners thought the north–south and south–south partnerships that evolved during the project were the main achievement. However, others found there to be a ‘north–south divide’ in certain aspects. Challenges included technical constraints and quality assurance. Additionally, adapting new teaching and learning methods into current university systems was challenging, combined with not being able to award students with credits for their degrees. Conclusion: The ARCADE projects were introduced as an innovative and ambitious project idea, although not designed

  12. Geographic distribution of habitat, development, and population growth rates of the Asian citrus psyllid, Diaphorina citri, in Mexico.

    Science.gov (United States)

    López-Collado, José; Isabel López-Arroyo, J; Robles-García, Pedro L; Márquez-Santos, Magdalena

    2013-01-01

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is an introduced pest in Mexico and a vector of huanglongbing, a lethal citrus disease. Estimations of the habitat distribution and population growth rates of D. citri are required to establish regional and areawide management strategies and can be used as a pest risk analysis tools. In this study, the habitat distribution of D. citri in Mexico was computed with MaxEnt, an inductive, machine-learning program that uses bioclimatic layers and point location data. Geographic distributions of development and population growth rates were determined by fitting a temperature-dependent, nonlinear model and projecting the rates over the target area, using the annual mean temperature as the predictor variable. The results showed that the most suitable regions for habitat of D. citri comprise the Gulf of Mexico states, Yucatán Peninsula, and areas scattered throughout the Pacific coastal states. Less suitable areas occurred in northern and central states. The most important predictor variables were related to temperature. Development and growth rates had a distribution wider than habitat, reaching some of the northern states of México. Habitat, development, and population growth rates were correlated to each other and with the citrus producing area. These relationships indicated that citrus producing states are within the most suitable regions for the occurrence, development, and population growth of D. citri, therefore increasing the risk of huanglongbing dispersion.

  13. Forecasting Spring Emergence of the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera: Crambidae), Based on Postdiapause Development Rate.

    Science.gov (United States)

    Park, Chang-Gyu; Seo, Bo Yoon; Jung, Jin Kyo; Kim, Hwang-Yong; Lee, Si-Woo; Seong, Ki Yeong

    2017-12-05

    This study was conducted to develop temperature-dependent postdiapause development models of overwintering larvae of the Asian corn borer, Ostrinia furnacalis Guenée (Lepidoptera: Crambidae), and to evaluate the models' forecasting accuracy using spring adult emergence data. Overwintering larvae were collected at three different times: 24 February (first), 23 March (second), and 25 April (third), 2005. The developmental periods of each collection colony were measured at eight constant temperatures, and those developmental rates were modeled with linear and nonlinear regression. One linear and three nonlinear models provided good fits of developmental rate to temperature across all colonies (r2 = 0.96-0.99). The distribution of development completion time was modeled with a Weibull equation that fit data from the second (r2 = 0.92) and third (r2 = 0.97) colonies better than the first (r2 = 0.87). A Lactin 2 model based on data from the first colony was statistically the best model to describe the relationship between temperature and the postdiapause development rate of O. furnacalis (r2adj = 0.99). However, validation results based on the field data showed that the Logan 6 model combined with the Weibull model (based on the second colony) was well describing spring adult emergence patterns up to 50% cumulative emergence date. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  14. Multinationals and East Asian Integration | IDRC - International ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    In Multinationals and East Asian Integration, leading Asian economists examine the role of foreign private-sector firms, especially from Japan and the United States, in the recent and rapid economic growth and integration in East Asia. With examples from China, Hong Kong, Indonesia, Malaysia, the Philippines, Singapore, ...

  15. Development of rapid phenotypic system for the identification of ...

    Indian Academy of Sciences (India)

    2013-04-20

    Apr 20, 2013 ... Infectious diseases are major cause of mortality in developing countries. The statistical data ... Gram-negative oxidase-positive non- fermenting bacilli are known for their ability to cause infection ..... Badreddine S 2009 Misdiagnosis of an imported case of malaria caused by Plasmodium falciparum. J. Infect.

  16. Rapid development of paper-based fluidic diagnostic devices

    CSIR Research Space (South Africa)

    Smith, S

    2014-11-01

    Full Text Available DEVELOPMENT OF PAPER-BASED FLUIDIC DIAGNOSTIC DEVICES S. Smith1*, H. Chen2, K. Moodley3, T. Joubert4 & K. Land5 1-5Department of Materials Science and Manufacturing Council for Scientific and Industrial Research, South Africa 1ssmith@csir.co.za,2jchen...

  17. Developing and evaluating rapid field methods to estimate peat carbon

    Science.gov (United States)

    Rodney A. Chimner; Cassandra A. Ott; Charles H. Perry; Randall K. Kolka

    2014-01-01

    Many international protocols (e.g., REDD+) are developing inventories of ecosystem carbon stocks and fluxes at country and regional scales, which can include peatlands. As the only nationally implemented field inventory and remeasurement of forest soils in the US, the USDA Forest Service Forest Inventory and Analysis Program (FIA) samples the top 20 cm of organic soils...

  18. Open dumping site in Asian developing countries: a potential source of polychlorinated dibenz-p-dioxins and polychlorinated dibenzofurans.

    Science.gov (United States)

    Nguyen, Hung Minh; Tu, Binh Minh; Watanabe, Mafumi; Kunisue, Tatsuya; Monirith, In; Tanabe, Shinsuke; Sakai, Shinichi; Subramanian, Annamalai; Sasikumar, Karuppian; Pham, Hung Viet; Bui, Cach Tuyen; Tana, Touch S; Prudente, Maricar S

    2003-04-15

    Open landfill dumping areas for municipal wastes in Asian developing countries have recently received particular attention with regard to environmental pollution problems. Because of the uncontrolled burning of solid wastes, elevated contamination by various toxic chemicals including dioxins and related compounds in these dumping sites has been anticipated. In this study, concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and coplanar polychlorinated biphenyls (PCBs) were determined in soils from dumping sites in the Philippines, Cambodia, India, and Vietnam. Residue concentrations of PCDD/Fs and coplanar PCBs in dumping site soils were apparently greater than those in soils collected in agricultural or urban areas far from dumping sites, suggesting that dumping sites are potential sources of PCDD/Fs and related compounds. Observed PCDD/F concentrations in soils from dumping sites in the Philippines and Cambodia were comparable or higher than those reported for dioxin-contaminated locations in the world (e.g., near the municipal waste incinerators and open landfill dumping sites). Homologue profiles of PCDD/Fs in dumping site soils from the Philippines and, to a lesser extent, from Cambodia and India reflected patterns of samples representing typical emissions, while profiles of agricultural or urban soils were similar to those of typical environmental sinks. This result suggests recent formation of PCDD/Fs in dumping site areas and that open dumping sites are a potential source of dioxins in Asian developing countries. Uncontrolled combustions of solid wastes by waste pickers, generation of methane gas, and low-temperature burning can be major factors for the formation of dioxins in dumping sites. Elevated fluxes of PCDD/Fs to soils in dumping sites were encountered in the Philippines, Cambodia, India, and Vietnam-Hanoi, and these levels were higher than those reported for other countries. Considerable loading rates

  19. Silencing abnormal wing disc gene of the Asian citrus psyllid, Diaphorina citri disrupts adult wing development and increases nymph mortality.

    Directory of Open Access Journals (Sweden)

    Ibrahim El-Shesheny

    Full Text Available Huanglongbing (HLB causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP, the vector of the bacterium, Candidatus Liberibacter asiaticus (CLas, the causal agent of HLB. Silencing genes by RNA interference (RNAi is a promising tool to explore gene functions as well as control pests. In the current study, abnormal wing disc (awd gene associated with wing development in insects is used to interfere with the flight of psyllids. Our study showed that transcription of awd is development-dependent and the highest level was found in the last instar (5(th of the nymphal stage. Micro-application (topical application of dsRNA to 5(th instar of nymphs caused significant nymphal mortality and adult wing-malformation. These adverse effects in ACP were positively correlated with the amounts of dsRNA used. A qRT-PCR analysis confirmed the dsRNA-mediated transcriptional down-regulation of the awd gene. Significant down-regulation was required to induce a wing-malformed phenotype. No effect was found when dsRNA-gfp was used, indicating the specific effect of dsRNA-awd. Our findings suggest a role for awd in ACP wing development and metamorphosis. awd could serve as a potential target for insect management either via direct application of dsRNA or by producing transgenic plants expressing dsRNA-awd. These strategies will help to mitigate HLB by controlling ACP.

  20. Population and development beyond the first demographic transition: a focus on the experience of east and southeast Asian countries

    Directory of Open Access Journals (Sweden)

    Gavin W. Jones

    2011-12-01

    Full Text Available This paper covers a wide scope, focusing on some trends in East and Southeast Asia that may be of interest to Latin America. The first demographic transition has essentially been completed in both regions. The issue is what should now be the focus of our consideration of population and development? East Asian countries are now stressing issues of ultra-low fertility, and policies to raise fertility. They are not comfortable with the prospect of making up future deficits through international migration. The paper also deals briefly with studies of dynamics of change in mega-urban regions, and argues that comparative studies on Latin America and Asia could be valuable. Issues of poverty, development, and equity are then addressed, with particular emphasis on the role of education as a key to equality and development. One dilemma is that in East Asia, the generally commendable obsession with education is one factor making for very low levels of fertility. Finally, the paper touches on population and environmental issues.

  1. Enhancing water security in a rapidly developing shale gas region

    Directory of Open Access Journals (Sweden)

    Shannon Holding

    2017-06-01

    New hydrological insights for the region: Initiatives and tools enhancing water security in the region include strategic partnerships and stakeholder collaborations, policy and regulation development, and data collection and distribution efforts. The contributions and limitations of each of these are discussed. A vulnerability mapping framework is presented which addresses data gaps and provides a tool for decision-making surrounding risk to water quality from various hazards. An example vulnerability assessment was conducted for wastewater transport along pipeline and trucking corridors.

  2. Multilingual Phoneme Models for Rapid Speech Processing System Development

    Science.gov (United States)

    2006-09-01

    Frequency Cepstral Coefficients . . . . . . . 11 2.4.3 Pronunciation Lexicon or Dictionary . . . . . . 12 2.4.4 Language Model...also a requirement for a pronunciation dictionary, and time is required for algorithm development and for building the speech recognition system. To...down into nasals, plosives, fricatives, approx- imants, trills, and flaps. Vowels are broken down by where the tongue is positioned within the mouth

  3. Development of Social Intensity Database Using Asian International Input–Output Table for Social Life Cycle Assessment

    Directory of Open Access Journals (Sweden)

    Seksan Papong

    2016-11-01

    Full Text Available The social impacts of products and service life cycles are increasingly of interest among policy makers and stakeholders. Workers’ issues are considered to be a source of key inventory data to assess social impacts, and are crucial in moving towards social sustainability. There is a need to develop a social inventory database for evaluating social impacts of products and services. This study aimed at the development of a social intensity dataset using an input–output analysis framework. The 2005 Asian International input–output table is used in this work. Six social issues are considered: total employment, paid workers, vulnerable employment, wages, fatal, and non-fatal occupational injuries. To verify the acceptability of this study, an estimation of total social footprint deduced from final consumption rates was carried out. The social intensities associated with 10 countries and 76 economic sectors were constructed. The results show that the social intensities from cradle to gate the agricultural sector has the highest in terms of total employment and vulnerable employment. Meanwhile, the mining sector in China has a higher non-fatal and fatal occupational injuries than the agriculture sector, secondary sector, and tertiary sector. The public administration sector and the education and research sector had a higher wages intensity than any other sectors due to these sectors being labor intensive and having higher wages. The social intensity in terms of total employment, paid workers, vulnerable employment, non-fatal injuries, and fatal accident cases in the developing countries was higher than the developed countries whereas wages intensity in developing countries was lower than that of developed countries. The social footprints resulting from the final consumption of each country show that the social footprints had transferred from the developing countries to the developed countries. Exports from China to the USA, Japan, South Korea

  4. A new era of Asian urology: a SWOT analysis.

    Science.gov (United States)

    Kumar, Rajeev

    2016-11-01

    Economic prosperity and increasing connectivity have made Asia an emerging centre of growth in health care, including in the field of urology. Large and varied patient populations, the availability of a trained workforce, the use of English as a common communication language, and overall low costs have contributed to this change. Rapid growth of regional urological associations and journals has fuelled the aspirations and abilities of Asian urologists to not only keep abreast of but often lead the change in urological disease management. Asian urology has immense potential to expand in areas in which it currently lags behind, the most important being research. The increasing ability to travel aids in developing networks for collaboration. However, Asian urologists will need to look at sustainable models of engagement and temper the need for short-term results if these opportunities are to reach their maximal potential.

  5. Rapid and Reusable Text Visualization and Exploration Development with DELVE.

    Science.gov (United States)

    Harris, Daniel R; Kavuluru, Ramakanth; Jaromczyk, Jerzy W; Johnson, Todd R

    2017-01-01

    We present DELVE (Document ExpLoration and Visualization Engine), a framework for developing interactive visualizations as modular Web-applications to assist researchers with exploratory literature search. The goal for web-applications driven by DELVE is to better satisfy the information needs of researchers and to help explore and understand the state of research in scientific liter ature by providing immersive visualizations that both contain facets and are driven by facets derived from the literature. We base our framework on principles from user-centered design and human-computer interaction (HCI). Preliminary evaluations demon strate the usefulness of DELVE's techniques: (1) a clinical researcher immediately saw that her original query was inappropriate simply due to the frequencies displayed via generalized clouds and (2) a muscle biologist quickly learned of vocabulary differences found between two disciplines that were referencing the same idea, which we feel is critical for interdisciplinary work. We dis cuss the underlying category-theoretic model of our framework and show that it naturally encourages the development of reusable visualizations by emphasizing interoperability.

  6. Infrastructure for Rapid Development of Java GUI Programs

    Science.gov (United States)

    Jones, Jeremy; Hostetter, Carl F.; Wheeler, Philip

    2006-01-01

    The Java Application Shell (JAS) is a software framework that accelerates the development of Java graphical-user-interface (GUI) application programs by enabling the reuse of common, proven GUI elements, as distinguished from writing custom code for GUI elements. JAS is a software infrastructure upon which Java interactive application programs and graphical user interfaces (GUIs) for those programs can be built as sets of plug-ins. JAS provides an application- programming interface that is extensible by application-specific plugins that describe and encapsulate both specifications of a GUI and application-specific functionality tied to the specified GUI elements. The desired GUI elements are specified in Extensible Markup Language (XML) descriptions instead of in compiled code. JAS reads and interprets these descriptions, then creates and configures a corresponding GUI from a standard set of generic, reusable GUI elements. These elements are then attached (again, according to the XML descriptions) to application-specific compiled code and scripts. An application program constructed by use of JAS as its core can be extended by writing new plug-ins and replacing existing plug-ins. Thus, JAS solves many problems that Java programmers generally solve anew for each project, thereby reducing development and testing time.

  7. Human resource development: the Asian experience in employment and manpower planning - an overview

    OpenAIRE

    Amjad, Rashid

    1987-01-01

    The overview based on country studies included in this volume investigates the extent to which it has been possible to integrate human resource development and give priority to this important goal within the overall planning process. While it is clear that the development of human resources has to compete with other pressing demands for limited resources available for development, the key question still remains whether overall socioeconomic development undertaken has incorporated the preferre...

  8. Development of Tailored Intervention to Promote Breast Cancer Screening Among Immigrant Asian Women Residing in the U.S

    National Research Council Canada - National Science Library

    Wu, Tsu-Yin

    2006-01-01

    Breast cancer is the leading diagnosed cancer in Asian American women. American women are more likely to receive a diagnosis in the advanced stages of the disease primarily because of late detection...

  9. Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons

    DEFF Research Database (Denmark)

    Kamara, David A; Nielsen, Lene Ryom; Ross, Michael

    2014-01-01

    No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90ml/min/1.73m2 (using Cockcroft Gault) in the Data Collec...

  10. Parallel grid library for rapid and flexible simulation development

    Science.gov (United States)

    Honkonen, I.; von Alfthan, S.; Sandroos, A.; Janhunen, P.; Palmroth, M.

    2013-04-01

    We present an easy to use and flexible grid library for developing highly scalable parallel simulations. The distributed cartesian cell-refinable grid (dccrg) supports adaptive mesh refinement and allows an arbitrary C++ class to be used as cell data. The amount of data in grid cells can vary both in space and time allowing dccrg to be used in very different types of simulations, for example in fluid and particle codes. Dccrg transfers the data between neighboring cells on different processes transparently and asynchronously allowing one to overlap computation and communication. This enables excellent scalability at least up to 32 k cores in magnetohydrodynamic tests depending on the problem and hardware. In the version of dccrg presented here part of the mesh metadata is replicated between MPI processes reducing the scalability of adaptive mesh refinement (AMR) to between 200 and 600 processes. Dccrg is free software that anyone can use, study and modify and is available at https://gitorious.org/dccrg. Users are also kindly requested to cite this work when publishing results obtained with dccrg. Catalogue identifier: AEOM_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEOM_v1_0.html Program obtainable from: CPC Program Library, Queen’s University, Belfast, N. Ireland Licensing provisions: GNU Lesser General Public License version 3 No. of lines in distributed program, including test data, etc.: 54975 No. of bytes in distributed program, including test data, etc.: 974015 Distribution format: tar.gz Programming language: C++. Computer: PC, cluster, supercomputer. Operating system: POSIX. The code has been parallelized using MPI and tested with 1-32768 processes RAM: 10 MB-10 GB per process Classification: 4.12, 4.14, 6.5, 19.3, 19.10, 20. External routines: MPI-2 [1], boost [2], Zoltan [3], sfc++ [4] Nature of problem: Grid library supporting arbitrary data in grid cells, parallel adaptive mesh refinement, transparent remote neighbor data updates and

  11. State and Federal project development procedures for bus rapid transit : managing differences and reducing implementation delays

    Science.gov (United States)

    2011-08-01

    This report documents an investigation into the transportation project development process in the : context of the implementation of bus rapid transit systems on the State Highway System as well as such : systems being part of the Federal New Starts ...

  12. Rapidly developing renal milk of calcium in a patient with myelomonocytic leukaemia

    OpenAIRE

    Morjaria, Anika; Balasubramaniam, Gowrie; Perera, Saman; Almond, Mike K

    2014-01-01

    Rapidly developing renal milk of calcium, diagnosed by computed tomography (CT), X-ray and ultrasound, should be considered as a rare differential diagnosis in patients with apparent ureteric obstruction to prevent unnecessary interventions.

  13. Development and Interpretation of New Sediment Rating Curve Considering the Effect of Vegetation Cover for Asian Basins

    Directory of Open Access Journals (Sweden)

    Jie Wang

    2013-01-01

    Full Text Available Suspended sediment concentration of a river can provide very important perspective on erosion or soil loss of one river basin ecosystem. The changes of land use and land cover, such as deforestation or afforestation, affect sediment yield process of a catchment through changing the hydrological cycle of the area. A sediment rating curve can describe the average relation between discharge and suspended sediment concentration for a certain location. However, the sediment load of a river is likely to be undersimulated from water discharge using least squares regression of log-transformed variables and the sediment rating curve does not consider temporal changes of vegetation cover. The Normalized Difference Vegetation Index (NDVI can well be used to analyze the status of the vegetation cover well. Thus long time monthly NDVI data was used to detect vegetation change in the past 19 years in this study. Then monthly suspended sediment concentration and discharge from 1988 to 2006 in Laichau station were used to develop one new sediment rating curve and were validated in other Asian basins. The new sediment model can describe the relationship among sediment yield, streamflow, and vegetation cover, which can be the basis for soil conservation and sustainable ecosystem management.

  14. Development and interpretation of new sediment rating curve considering the effect of vegetation cover for Asian basins.

    Science.gov (United States)

    Wang, Jie; Ishidaira, Hiroshi; Sun, Wenchao; Ning, Shaowei

    2013-01-01

    Suspended sediment concentration of a river can provide very important perspective on erosion or soil loss of one river basin ecosystem. The changes of land use and land cover, such as deforestation or afforestation, affect sediment yield process of a catchment through changing the hydrological cycle of the area. A sediment rating curve can describe the average relation between discharge and suspended sediment concentration for a certain location. However, the sediment load of a river is likely to be undersimulated from water discharge using least squares regression of log-transformed variables and the sediment rating curve does not consider temporal changes of vegetation cover. The Normalized Difference Vegetation Index (NDVI) can well be used to analyze the status of the vegetation cover well. Thus long time monthly NDVI data was used to detect vegetation change in the past 19 years in this study. Then monthly suspended sediment concentration and discharge from 1988 to 2006 in Laichau station were used to develop one new sediment rating curve and were validated in other Asian basins. The new sediment model can describe the relationship among sediment yield, streamflow, and vegetation cover, which can be the basis for soil conservation and sustainable ecosystem management.

  15. A Review of Recent Developments in the Study of Regional Lithospheric Electrical Structure of the Asian Continent

    Science.gov (United States)

    Zhang, Letian

    2017-09-01

    The Asian continent was formed through the amalgamation of several major continental blocks that were formerly separated by the Paleo-Asian and Tethyan Oceans. During this process, the Asian continent underwent a long period of continental crustal growth and tectonic deformation, making it the largest and youngest continent on Earth. This paper presents a review of the application of geophysical electromagnetic methods, mainly the magnetotelluric (MT) method, in recent investigations of the diverse tectonic features across the Asian continent. The case studies cover the major continental blocks of Asia, the Central Asian orogenic system, the Tethyan orogenic system, as well as the western Pacific subduction system. In summary, most of the major continental blocks of Asia exhibit a three-layer structure with a resistive upper crust and upper mantle and a relatively conductive mid-lower crust. Large-scale conductors in the upper mantle were interpreted as an indication of lithospheric modification at the craton margins. The electrical structure of the Central Asian orogenic system is generally more resistive than the bordering continental blocks, whereas the Tethyan orogenic system displays more conductive, with pervasive conductors in the lower crust and upper mantle. The western Pacific subduction system shows increasing complexity in its electrical structure from its northern extent to its southern extent. In general, the following areas of the Asian continent have increasingly conductive lithospheric electrical structures, which correspond to a transition from the most stable areas to the most active tectonic areas of Asia: the major continental blocks, the accretionary Central Asian orogenic system, the collisional Tethyan orogenic system, and the western Pacific subduction system. As a key part of this review, a three-dimensional (3-D) model of the lithospheric electrical structure of a large portion of the Tibetan Plateau is presented and discussed in detail

  16. Missing Millions and Measuring Progress towards the Millenium Development Goals with a focus on Central Asian States

    Directory of Open Access Journals (Sweden)

    Roy Carr-Hill

    2012-07-01

    Full Text Available Background In developing countries, population estimates and assessments of progress towards the Millenium Development Goals are based increasingly on household surveys.  It is not recognised that they are inappropriate for obtaining information about the poorest of the poor..  This is because they, typically, omit by design: those not in households because they are homeless; those who are in institutions; and mobile, nomadic or pastoralist populations.  In addition, in practice, because they are difficult to reach, household surveys will typically under-represent: those in fragile, disjointed or multiple occupancy households; those in urban slums, and may omit certain areas of a country deemed to pose a security risk. Those six sub-groups constitute a pretty comprehensive ostensive definition of the ‘poorest of the poor’. Methods This paper documents these omissions in general, drawing on worldwide literature about the theory and practice fo implementing censuses and household surveys; and shows how substantial proportions are missing from both censuses and the sample frames of surveys. Results This paper suggests that between 300 and 350 million will effectively be missed worldwide from the sampling frames of such surveys and from most censuses.  The impact on the health MDGs is illustrated for the five republics of the former Soviet Union making up Central Asia: Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan and Uzbekistan Conclusions It is impossible to assess progress towards or away from the MDGS in both the Central Asian Republics and worldwide.  It is urgent to find solutions to the problem of the ‘missing ‘ poor population sub-groups.

  17. Development of alternative methods of data collection in South Asians with Type 2 diabetes.

    Science.gov (United States)

    Lloyd, C E; Sturt, J; Johnson, M; Mughal, S; Collins, G; Barnett, A H

    2008-04-01

    Previous research in the UK has established the difficulty of recruiting and collecting information from individuals whose main language is spoken and does not have an agreed written form. The aims of this study were (i) to develop culturally competent translations of two questionnaires measuring diabetes self-care in languages with no written form and to establish their face validity and (ii) to develop acceptable methods of delivery with the potential for providing valid and reliable data for use in research studies. Adults with Type 2 diabetes from two minority ethnic groups whose main language is spoken (Sylheti and Mirpuri) were recruited via the Birmingham Heartlands hospital diabetes centre. Participants were invited to attend five focus groups to consider the content and method of delivery of two questionnaires measuring knowledge of diabetes and confidence in diabetes self-care. Delivery methods were (i) pen and paper self-completion in Bengali/Urdu, (ii) pen and paper assisted completion in spoken language, (iii) partially-assisted completion in spoken language, (iv) independent audio delivery in spoken language. Culturally competent content was achieved for both questionnaires in both languages. The Mirpuri men and women's groups preferred assisted or partially assisted completion in spoken language. The Sylheti groups' preference was for independent audio delivery in spoken language. The face validity of two questionnaires measuring diabetes self-care is established for Sylheti and Mirpuri, in four alternative delivery formats. The questionnaires are now ready for psychometric testing in minority ethnic populations and the methods available for use by researchers to establish within-study feasibility.

  18. Usages of Computers and Smartphones to Develop Dementia Care Education Program for Asian American Family Caregivers.

    Science.gov (United States)

    Lee, Jung-Ah; Nguyen, Hannah; Park, Joan; Tran, Linh; Nguyen, Trang; Huynh, Yen

    2017-10-01

    Families of ethnic minority persons with dementia often seek help at later stages of the disease. Little is known about the effectiveness of various methods in supporting ethnic minority dementia patients' caregivers. The objective of the study was to identify smartphone and computer usage among family caregivers of dementia patients (i.e., Korean and Vietnamese Americans) to develop dementia-care education programs for them. Participants were asked various questions related to their computer or smartphone usage in conjunction with needs-assessment interviews. Flyers were distributed at two ethnic minority community centers in Southern California. Snowball recruitment was also utilized to reach out to the families of dementia patients dwelling in the community. Thirty-five family caregivers, including 20 Vietnamese and 15 Korean individuals, participated in this survey. Thirty participants (30 of 35, 85.7%) were computer users. Among those, 76.7% (23 of 30) reported daily usage and 53% (16 of 30) claimed to use social media. A majority of the participants (31 of 35, 88.6%) reported that they owned smartphones. More than half of smartphone users (18 of 29, 62%) claimed to use social media applications. Many participants claimed that they could not attend in-class education due to caregiving and/or transportation issues. Most family caregivers of dementia patients use smartphones more often than computers, and more than half of those caregivers communicate with others through social media apps. A smartphone-app-based caregiver intervention may serve as a more effective approach compared to the conventional in-class method. Multiple modalities for the development of caregiver interventions should be considered.

  19. Development and evaluation of multimedia interventions to promote breast and cervical health among South Asian women in Hong Kong: A project protocol

    Directory of Open Access Journals (Sweden)

    Winnie K. W. So

    2017-01-01

    Full Text Available Recent studies conducted in the local community indicate that the uptake rates of breast and cervical cancer screening among South Asian ethnic minorities are lower than those of the general population. The development of interventions to promote these minorities' awareness of breast and cervical health and the importance of cancer screening is therefore required. This study protocol aims to develop culturally sensitive multimedia interventions to promote awareness of breast and cervical cancer prevention among South Asian women in Hong Kong, and to evaluate the outcomes of such interventions using a Reach-Effectiveness-Adoption-Implementation-Maintenance framework. By using a multimedia approach and developing socio-culturally relevant and linguistically appropriate educational materials, information related to cancer and accessible preventive measures for breast and cervical cancer is expected to be disseminated more effectively among South Asian women and ultimately increase their awareness of engaging in healthy lifestyles and taking part in cancer screening tests. Successful engagement of community partners will enhance the future sustainability of the project.

  20. Community pharmacy and emerging public health initiatives in developing Southeast Asian countries: a systematic review.

    Science.gov (United States)

    Hermansyah, Andi; Sainsbury, Erica; Krass, Ines

    2016-09-01

    The development of health and healthcare systems in South-East Asia has influenced the practice of community pharmacy. Over the years, community pharmacy in the region has striven to expand services beyond dispensing to encompass more involvement in public health issues. Searches were conducted in Scopus, EMBASE, MEDLINE and PubMed for articles published between January 2000 and December 2014, with 21 studies in five countries meeting the inclusion criteria. The findings showed increasing interest in research into the delivery of pharmacy services and public health initiatives. Overall, the review found that provision of some health services in pharmacies was common; however, most public health initiatives appeared to be poorly implemented, had limited evidence and were not demonstrated to be sustainable across the sector. This indicates that the practice of community pharmacy in the region has not significantly changed over the past 14 years with respect to the scope and quality of pharmacy services provided, and fundamental policy changes are necessary to improve this situation. © 2015 John Wiley & Sons Ltd.

  1. Impact of ICT usage on indigenous peoples’ quality of life: Evidence from an Asian developing country

    Directory of Open Access Journals (Sweden)

    Md Mahfuz Ashraf

    2015-11-01

    Full Text Available Indigenous communities across the world have been suffering disadvantages in several domains, e.g. erosion of land rights, language and other cultural aspects, while at the same time being discriminated against when prepared to integrate into the dominant cultures. It has been argued in the literature that information communication technologies (ICTs have the potential of contributing to addressing some of these disadvantages – both in terms of rebuilding what has been eroded and facilitating integration into non-Indigenous societies. In trying to understand how ICTs can be useful for these processes, it is important to do so from a conceptual framework that encompasses the multi-dimensionality of the issues faced by Indigenous communities. The conceptual frameworks frequently used in the ICT literature tend to focus on adoption, use and diffusion of technologies rather than how the use of ICTs affects the livelihoods of the users, which is the focus of this paper. The conceptual framework is informed by the capability approach (CA, in particular by the five freedoms identified in the seminal work of Amartya Sen (2001, “Development as Freedom” (DaF. Data were collected from a purposive sample in an Indigenous community in Bangladesh, using a qualitative method to map how ICTs had affected the lives of these community members The findings suggest that the participants perceived that ICTs had made positive contributions, particularly the benefits they gained from learning how to use computers in the domains that are relevant from the perspective of the five freedoms espoused in DaF. The findings reported in this paper are useful for policy formulation in Bangladesh. As the study is contextualised in a transitional economy setting and can therefore not be generalised, but we believe that the conceptual framework has much to offer future research designed to understand how ICTs can improve the livelihoods of Indigenous individuals and

  2. Rapid Hip Osteoarthritis Development in a Patient with Anterior Acetabular Cyst with Sagittal Alignment Change

    Directory of Open Access Journals (Sweden)

    Yasuhiro Homma

    2014-01-01

    Full Text Available Rapidly destructive coxarthrosis (RDC is rare and develops unusual clinical course. Recent studies suggest multiple possible mechanisms of the development of RDC. However the exact mechanism of RDC is still not clear. The difficulty of the study on RDC is attributed to its rareness and the fact that the data before the onset of RDC is normally unavailable. In this report, we presented the patient having the radiographic data before the onset who had rapid osteoarthritis (OA development after contralateral THA, which meets the current criteria of RDC. We thought that the increased posterior tilt of the pelvis after THA reinforced the stress concentration at pre-existed anterior acetabular cyst, thereby the destruction of the cyst was occurred. As a result the rapid OA was developed. We think that there is the case of rapid osteoarthritis developing due to alternating load concentration by posterior pelvic tilt on preexisting anterior acetabular cyst such as our patient among the cases diagnosed as RDC without any identifiable etiology. The recognition of sagittal alignment changes and anterior acetabular cyst may play important role in prediction and prevention of the rapid hip osteoarthritis development similar to RDC.

  3. The Relationship between the Movements of Capital Markets in Developed Economies and Their Emerging Market Counterparts in The Asian Paciic Region

    Directory of Open Access Journals (Sweden)

    Noor Azlinna Azizan

    2014-08-01

    Full Text Available This research revisits at the relationship between the movements of capital markets in developed  economies  and  their  emerging  market  counterparts  in  the  Asian  Paciic  region using market indices of the American, British, Malaysian, Singaporean, Mainland Chinese, Hong Kong Special Administrative Region (SAR, Indian, Japanese and Australian markets for  the  periods  1997  to  2007.  The  Johansen's  Cointegration  Test,  and  Vector  Correction Model  Test  were  used  to  determine  the  long  term  relationship  between  the  markets.  This study inds that the Asian markets are very much inluenced by the events in the United States rather  than  other  developed  markets.  Of  all  the  markets  being  surveyed,  The  South  East Asian markets are the most sensitive towards events in their own region and regions outside themselves. Mainland China in the long run is not affected by events outside themselves.  ";} // -->activate javascript

  4. Development of SCAR Markers for the DNA-Based Detection of the Asian Long-Horned Beetle; Anoplophora glabripennis (Motschulsky)

    Science.gov (United States)

    Damodar R. Kethidi; David B. Roden; Tim R. Ladd; Peter J. Krell; Arthur Ratnakaran; Qili Feng

    2003-01-01

    DNA markers were identified for the molecular detection of the Asian long-horned beetle (ALB), Anoplophora glabripennis (Mot.), based on sequence charaterized amplified regions (SCARS) derived from random amplified polymorphic DNA (RAPD) fragments. A 2,740-bp DNA fragment that was present only in ALB and not in other Cerambycids was identified after...

  5. Formulation of Policy and Strategy in Developing Creativity Education in Four Asian Chinese Societies: A Policy Analysis

    Science.gov (United States)

    Hui, Anna N. N.; Lau, Sing

    2010-01-01

    The present study sought to compare and contrast educational policies on creativity education in four Asian Chinese societies, namely mainland China, Hong Kong, Singapore, and Taiwan. It establishes five criteria on creativity education policy, including policies regarding legislation on creativity education, definitions of creativity, standard…

  6. East Asian welfare regime

    DEFF Research Database (Denmark)

    Abrahamson, Peter

    2017-01-01

    The paper asks if East Asian welfare regimes are still productivist and Confucian? And, have they developed public care policies? The literature is split on the first question but (mostly) confirmative on the second. Care has to a large, but insufficient extent, been rolled out in the region. Pol...... focusing on outcomes or causal links tend to suggest that legacies prevail, but there is (nearly) consensus that Confucianism exercises great influence in the whole region....

  7. Sustainable energy for the future. Modelling transitions to renewable and clean energy in rapidly developing countries.

    NARCIS (Netherlands)

    Urban, Frauke

    2009-01-01

    The main objective of this thesis is first to adapt energy models for the use in developing countries and second to model sustainable energy transitions and their effects in rapidly developing countries like China and India. The focus of this thesis is three-fold: a) to elaborate the differences

  8. Perspectives in asian rhinoplasty.

    Science.gov (United States)

    Jang, Yong Ju; Yi, Jong Sook

    2014-04-01

    Asian patients present with relatively poorly developed dorsal and tip height and thicker skin, so augmentation rhinoplasty is the most commonly performed rhinoplasty procedure. Tip surgery using autologous cartilage followed by dorsal augmentation using an alloplastic implant material is the most widely performed surgical procedure for augmentation rhinoplasty on Asian patients. Cartilage tip grafting procedures, including shield grafting, multilayer tip grafting, onlay grafting, and modified vertical dome division, are key maneuvers for building up and providing better definition on a relatively poorly developed Asian tip. When performing primary cosmetic dorsal augmentation using alloplastic implants, the implant material should be selected according to the surgeon's experience, the pros and cons of available dorsal implant materials, and host factors such as skin thickness, associated deformities, and aesthetic goals. The costal cartilage is best reserved for difficult revisions, except in a limited number of primary cases who present with a very poorly developed nasal skeleton and thick skin. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

  9. Considerations for Task Analysis Methods and Rapid E-Learning Development Techniques

    Directory of Open Access Journals (Sweden)

    Dr. Ismail Ipek

    2014-02-01

    Full Text Available The purpose of this paper is to provide basic dimensions for rapid training development in e-learning courses in education and business. Principally, it starts with defining task analysis and how to select tasks for analysis and task analysis methods for instructional design. To do this, first, learning and instructional technologies as visions of the future were discussed. Second, the importance of task analysis methods in rapid e-learning was considered, with learning technologies as asynchronous and synchronous e-learning development. Finally, rapid instructional design concepts and e-learning design strategies were defined and clarified with examples, that is, all steps for effective task analysis and rapid training development techniques based on learning and instructional design approaches were discussed, such as m-learning and other delivery systems. As a result, the concept of task analysis, rapid e-learning development strategies and the essentials of online course design were discussed, alongside learner interface design features for learners and designers.

  10. Young Asian Dutch constructing Asianness: Understanding the role of Asian popular culture

    OpenAIRE

    Kartosen, R.A.

    2016-01-01

    This doctoral thesis is about young Asian Dutch, panethnic Asian identities and identifications, and Asian/Asian Dutch popular culture. It addresses several pressing questions, including: why do young Asian Dutch, who were born and/or raised in the Netherlands, identify as Asian and construct Asian identities? What is the content or meaning of these Asian identities and identifications young Asian Dutch imagine? And how do these relate to young Asian Dutch’ Dutch and homeland identities and i...

  11. [Development of a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method].

    Science.gov (United States)

    Yang, Fa-qing; Lee, Tony; Wang, Chao-nan; Sun, Shu-ye; Li, Shan-shan; Tian, Hui

    2010-06-01

    To develop a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method. A rapid test kit was developed by conjugation of the HIV antigen gp41 and gp36 to 200nm super paramagnetic particles by carbodiimide (EDC) and coating of the HIV antigen gp41 and gp36 to nitrocellulose membrane. Then the kit was evaluated with serials of experiments. The kit was qualified with examination of national reference panel of anti-HIV antibody for colloidal gold diagnostic kit. The sensitivity was 100% by tested with 20 HIV antibody positive sera, the specificity was 98.5% by tested with 600 HIV antibody negative sera, respectively. The stability of the kit was over 12 month by storage at room temperature. A diagnostic kit for antibody to HIV was developed with the advantages of convenience, rapid test, good stability and point of care.

  12. Rapid E-learning Development Strategies and a Multimedia Project Design Model

    OpenAIRE

    Ömer Faruk Sözcü; İsmail İpek

    2014-01-01

    The purpose of the study is to discuss e-learning design strategies which can be used for multimedia projects as a design model. Recent advances in instructional technologies have been found to be very important in the design of training courses by using rapid instructional design (ID) approaches. The approaches were developed to use in training and developing e-learning projects as well as multimedia design tools. For this reason, this paper will clarify the basic e-learning development tech...

  13. Pathological study of chronic pulmonary toxicity induced by intratracheally instilled Asian sand dust (Kosa): possible association of fibrosis with the development of granulomatous lesions.

    Science.gov (United States)

    Shimada, Akinori; Kohara, Yukari; Naota, Misaki; Kobayashi, Yoshimi; Morita, Takehito; Inoue, Kenichiro; Takano, Hirohisa

    2015-01-01

    Exposure to Asian sand dust (ASD) is associated with enhanced pulmonary morbidity and mortality, and the reporting of such cases has rapidly increased in East Asia since 2000. The purpose of the study was to assess chronic lung toxicity induced by ASD. A total of 174 ICR mice were randomly divided into 5 control and 17 exposure groups. Suspensions of low dose (0.2, 0.4 mg) and high dose (3.0 mg) of ASD particles in saline were intratracheally instilled into ICR mice, followed by sacrifice at 24 hours, 1 week, and 1, 2, 3 and 4 months after instillation. Paraffin sections of lung tissues were stained with hematoxylin and eosin and by immunohistochemistry to detect α-smooth muscle actin, collagen III, matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinases-1 (TIMP-1), CD3, CD20, immunoglobulin G, interleukin-1β and inducible nitric oxide synthase. A lung histological examination revealed similar patterns in the lesions of the groups treated with high (3.0 mg) or low dose (0.4 mg) of ASD. Acute inflammation was observed 24 h after treatment and subsided after 1 week; persistent granulomatous changes were observed at 2 months, focal lymphocytic infiltration at 3 months, and granuloma formation at 4 months. An increase in the size of granulomatous lesions was observed over time and was accompanied by collagen deposition in the lesions. The cytoplasm of macrophages in inflammatory lesions showed positive immunolabeling for MMP-9 at 24 h, 1 and 2 months after instillation of 3.0 mg of ASD. Positive immunolabeling for TIMP-1 was demonstrated in the cytoplasm of macrophages at 2 and 4 months after instillation of 3.0 mg of ASD. These findings suggest association between the expression of MMP-9 and TIMP-1 with the development of lung granulomatous lesions. These findings suggest that collagen deposition resulting from the altered regulation of extracellular matrix is associated with granuloma formation in the lungs of mice treated with ASD.

  14. Rapid Development of Hybrid Courses for Distance Education: A Midwestern University's Pilot Project

    Science.gov (United States)

    Rust, Jodi

    2011-01-01

    A descriptive case study was used to explore how repurposing and a pedagogical-based instructional design model, the multimodal model (Picciano, 2009), were used to create quality distance education courses in a rapid development setting at a Midwestern land grant university. Data triangulation was used to secure data from faculty member…

  15. Rapid E-learning Development Strategies and a Multimedia Project Design Model

    Science.gov (United States)

    Sözcü, Ömer Faruk; Ipek, Ismail

    2014-01-01

    The purpose of the study is to discuss e-learning design strategies which can be used for multimedia projects as a design model. Recent advances in instructional technologies have been found to be very important in the design of training courses by using rapid instructional design (ID) approaches. The approaches were developed to use in training…

  16. Experiments in rapid development of accurate phonetic alignments for TTS in Afrikaans

    CSIR Research Space (South Africa)

    Van Niekerk, DR

    2011-11-01

    Full Text Available The quality of corpus-based text-to-speech (TTS) systems depends on the accuracy of phonetic annotation (alignments) which directly influences the process of acoustic modelling. In this paper the author discusses the rapid development of accurate...

  17. Development of flexural vibration inspection techniques to rapidly assess the structural health of rural bridge systems

    Science.gov (United States)

    Brian K. Brashaw; Robert Vatalaro; Xiping Wang; Kevin Sarvela; James P. Wacker

    2008-01-01

    Approximately 4,000 vehicle bridges in the State of Minnesota contain structural timber members. Recent research at the University of Minnesota Duluth Natural Resources Research Institute (UMD NRRI) has been conducted on vibration testing of timber bridges as a means of developing rapid in-place testing techniques for assessing the structural health of bridges. The...

  18. Did opening of the South China Sea impact development of the Asian Monsoon? Results from Oligocene microfossils, IODP Site U1435, northern South China Sea

    Science.gov (United States)

    Kulhanek, Denise K.; Su, Xin; Li, Qianyu; Gregory, Mitch; Warny, Sophie; Clift, Peter D.

    2016-04-01

    Development of the Asian Monsoon is linked to uplift of the Himalayas and Tibetan Plateau in the Cenozoic, with good evidence for a strong monsoon system by the late Oligocene to early Miocene (e.g., Guo et al., 2002; Clift et al., 2008). However, Licht et al. (2014) suggested the presence of an Asian Monsoon in the late Eocene. Recent scientific ocean drilling in the Indian Ocean and surrounding marginal seas gives us the opportunity to test this hypothesis with newly recovered Paleogene sediment cores. International Ocean Discovery Program Expedition 349 to the South China Sea recovered a 30 m section of primarily lower Oligocene nannofossil-rich claystone at Site U1435, located near the northern continent/ocean boundary. A thick sandstone unit devoid of typical marine microfossils underlies the marine claystone. The sandstone is interpreted as a deltaic or restricted marine deposit and is dated to the Eocene based on the presence of organic-walled palynomorphs, suggesting that a hiatus of several million years likely separates the sandstone below from the Oligocene marine claystone. This hiatus is interpreted as the breakup unconformity, with paleodepths in the South China Sea increasing during the Oligocene. Thus, this claystone should record if opening of the South China Sea during the early Oligocene influenced development of the Asian Monsoon. Combined calcareous nannofossil and planktonic foraminifer biostratigraphy indicates that the 30 m section is primarily early Oligocene in age (~33.5-30 Ma) and was deposited on the middle slope, with paleodepths >500 m. Stable oxygen isotopes from planktonic foraminifers become heavier up-hole, suggestive of cooling/deepening in the region, whereas carbon isotopes record variable conditions with no distinct maxima or minima. Calcareous nannoplankton primarily live in the upper 50 m of the ocean and are sensitive to sea-surface temperature and nutrient conditions, thus making them useful recorders of paleoceanographic

  19. Young Asian Dutch constructing Asianness: Understanding the role of Asian popular culture

    NARCIS (Netherlands)

    Kartosen, R.A.

    2016-01-01

    This doctoral thesis is about young Asian Dutch, panethnic Asian identities and identifications, and Asian/Asian Dutch popular culture. It addresses several pressing questions, including: why do young Asian Dutch, who were born and/or raised in the Netherlands, identify as Asian and construct Asian

  20. Effect of brief daily resistance training on rapid force development in painful neck and shoulder muscles

    DEFF Research Database (Denmark)

    Jay, Kenneth; Schraefel, Mc; Andersen, Christoffer H

    2013-01-01

    performed maximal voluntary contractions at a static 90-degree shoulder joint angle. Rapid force development was determined as the rate of torque development and maximal muscle strength was determined as the peak torque. RESULTS: Compared with the control group, rate of torque development increased 31.0 Nm......OBJECTIVE: To determine the effect of small daily amounts of progressive resistance training on rapid force development of painful neck/shoulder muscles. METHODS: 198 generally healthy adults with frequent neck/shoulder muscle pain (mean: age 43.1 years, computer use 93% of work time, 88% women.......05) for both training groups. Maximal muscle strength increased only ~5-6% [mean and 95% confidence interval for 2- and 12-min groups to control, respectively: 2.5 Nm (0.05-0.73) and 2.2 Nm (0.01-0.70)]. No significant differences between the 2- and 12-min groups were evident. A weak but significant...

  1. Development of Experimental Setup of Metal Rapid Prototyping Machine using Selective Laser Sintering Technique

    Science.gov (United States)

    Patil, S. N.; Mulay, A. V.; Ahuja, B. B.

    2016-08-01

    Unlike in the traditional manufacturing processes, additive manufacturing as rapid prototyping, allows designers to produce parts that were previously considered too complex to make economically. The shift is taking place from plastic prototype to fully functional metallic parts by direct deposition of metallic powders as produced parts can be directly used for desired purpose. This work is directed towards the development of experimental setup of metal rapid prototyping machine using selective laser sintering and studies the various parameters, which plays important role in the metal rapid prototyping using SLS technique. The machine structure in mainly divided into three main categories namely, (1) Z-movement of bed and table, (2) X-Y movement arrangement for LASER movements and (3) feeder mechanism. Z-movement of bed is controlled by using lead screw, bevel gear pair and stepper motor, which will maintain the accuracy of layer thickness. X-Y movements are controlled using timing belt and stepper motors for precise movements of LASER source. Feeder mechanism is then developed to control uniformity of layer thickness metal powder. Simultaneously, the study is carried out for selection of material. Various types of metal powders can be used for metal RP as Single metal powder, mixture of two metals powder, and combination of metal and polymer powder. Conclusion leads to use of mixture of two metals powder to minimize the problems such as, balling effect and porosity. Developed System can be validated by conducting various experiments on manufactured part to check mechanical and metallurgical properties. After studying the results of these experiments, various process parameters as LASER properties (as power, speed etc.), and material properties (as grain size and structure etc.) will be optimized. This work is mainly focused on the design and development of cost effective experimental setup of metal rapid prototyping using SLS technique which will gives the feel of

  2. Evolution of Asian Interior Arid-Zone Biota: Evidence from the Diversification of Asian Zygophyllum (Zygophyllaceae).

    Science.gov (United States)

    Wu, Sheng-Dan; Lin, Li; Li, Hong-Lei; Yu, Sheng-Xiang; Zhang, Lin-Jing; Wang, Wei

    2015-01-01

    The Asian interior arid zone is the largest desert landform system in the Northern Hemisphere, and has high biodiversity. Little is currently known about the evolutionary history of its biota. In this study, we used Zygophyllum, an important and characteristic component of the Asian interior arid zone, to provide new insights into the evolution of this biota. By greatly enlarged taxon sampling, we present the phylogenetic analysis of Asian Zygophyllum based on two plastid and one nuclear markers. Our phylogenetic analyses indicate that Asian Zygophyllum and Sarcozygium form a clade and Sarcozygium is further embedded within the shrub subclade. An integration of phylogenetic, biogeographic, and molecular dating methods indicates that Zygophyllum successfully colonized the Asian interior from Africa in the early Oligocene, and Asian Zygophyllum became differentiated in the early Miocene and underwent a burst of diversification in the late Miocene associated with the expansion of Asian interior arid lands due to orogenetic and climatic changes. Combining diversification patterns of other important components of the Asian interior arid zone, we propose a multi-stage evolution model for this biota: the late Eocene-early Oligocene origin, the early Miocene expansion, and the middle-late Miocene rapid expansion to the whole Asian interior arid zone. This study also demonstrates that, for Zygophyllum and perhaps other arid-adapted organisms, arid biomes are evolutionary cradles of diversity.

  3. READERS: Rapid English Assimilation Developing English Reading and Speaking; Social and Academic Implications of English Proficiency amongst Children

    Science.gov (United States)

    Seaton, Russell

    2017-01-01

    The READERS (Rapid English Assimilation Developing English Reading and Speaking) program is designed to facilitate teachers and students in rapid English assimilation, focusing on grammar and verbal recall as pertaining to the English language. The purpose of this qualitative method case study was to investigate the effects of rapid English…

  4. IL-6 trans-Signaling-Dependent Rapid Development of Cytotoxic CD8+ T Cell Function

    Directory of Open Access Journals (Sweden)

    Jan P. Böttcher

    2014-09-01

    Full Text Available Immune control of infections with viruses or intracellular bacteria relies on cytotoxic CD8+ T cells that use granzyme B (GzmB for elimination of infected cells. During inflammation, mature antigen-presenting dendritic cells instruct naive T cells within lymphoid organs to develop into effector T cells. Here, we report a mechanistically distinct and more rapid process of effector T cell development occurring within 18 hr. Such rapid acquisition of effector T cell function occurred through cross-presenting liver sinusoidal endothelial cells (LSECs in the absence of innate immune stimulation and known costimulatory signaling. Rather, interleukin-6 (IL-6 trans-signaling was required and sufficient for rapid induction of GzmB expression in CD8+ T cells. Such LSEC-stimulated GzmB-expressing CD8+ T cells further responded to inflammatory cytokines, eliciting increased and protracted effector functions. Our findings identify a role for IL-6 trans-signaling in rapid generation of effector function in CD8+ T cells that may be beneficial for vaccination strategies.

  5. Predictors of Bone Mineral Density among Asian Indians in Northern Mississippi: A Pilot Study.

    Science.gov (United States)

    Nahar, Vinayak K; Nelson, Kyle M; Ford, M Allison; Sharma, Manoj; Bass, Martha A; Haskins, Mary A; Garner, John C

    2016-01-01

    Osteoporosis is a systemic skeletal disorder characterized by low bone mineral density (BMD) that leads to an increase in bone fragility, causing an individual to be at an increased risk for fractures. Asian-Indians are at an increased risk for developing osteoporosis. Considering the number of Asian-Indians in the US is rapidly growing, they likely could be an underappreciated population at risk for bone fractures. The aim of this study was to investigate bone health and determine the factors affecting BMD in Asian-Indians living in the US. Asian-Indians residing in Northern Mississippi (n = 87) were enrolled in this cross-sectional study from June 2013 to August 2014. Eligible participants completed a self-administered Osteoporosis Risk Factor Assessment questionnaire. BMD and body composition were measured using a dual energy x-ray absorptiometry scan (DXA). Eight-seven Asian-Indians (male: 62.1%) participated, with the average age being 28.49 yr old (SD = 6.62). Overall, 31.0% and 48.3% had low femoral neck BMD and spinal BMD, respectively. Multiple regression analysis revealed that age, percent body fat, and body mass index (BMI) significantly predicted BMD at femur neck (PHealth promotion programs focusing on osteoporosis prevention are needed among Asian-Indians to prevent the risk of fractures.

  6. Rapid tolerance development to the NREM sleep promoting effect of alcohol.

    Science.gov (United States)

    Sharma, Rishi; Sahota, Pradeep; Thakkar, Mahesh M

    2014-04-01

    Alcohol tolerance is a major contributor towards the development of alcohol dependence. Does alcohol intake result in rapid tolerance development to alcohol induced NREM sleep promotion? This has never been examined. Our objective was to examine whether two bouts of alcohol consumption on consecutive days results in rapid tolerance development to alcohol-induced NREM sleep promotion. N/A. N/A. C57BL/6J mice. Mice (N = 5) were implanted with sleep electrodes using standard surgical conditions. Following postoperative recovery and habituation, the experiment was begun. On baseline day, water bottle changes were performed at 10:00 (3 h after dark onset) and 14:00 to mimic conditions during alcohol consumption days. On next 2 days, (Days 1 and 2) mice were allowed to self-administer alcohol (20% v/v) for 4 h beginning at 10:00 and ending at 14:00. Sleep-wakefulness was continuously recorded from 10:00 to 18:00 (8 h; 4 h during alcohol + 4 h post-alcohol) on all 3 days. Although mice consumed comparable amounts of alcohol on Days 1 and 2, NREM sleep and wakefulness were significantly and differentially affected during 4 h post-alcohol period. A robust alcohol-induced NREM sleep promotion was observed on Day 1. However, no such sleep promotion was observed on Day 2, suggesting rapid tolerance development. Our study is the first to demonstrate that alcohol consumption for two consecutive days results in development of rapid tolerance to alcohol-induced sleep promotion.

  7. Changing Asian American Stereotypes

    Science.gov (United States)

    Salvador-Burris, Juanita

    1978-01-01

    Social science literature on stereotypes is reviewed. Negative stereotypes of Asian Americans are examined and their relationship to Asian American political and economic status and self image is discussed. Specific actions to counter these stereotypes are advocated. (GC)

  8. Obesity and Asian Americans

    Science.gov (United States)

    ... and Data > Minority Population Profiles > Asian American > Obesity Obesity and Asian Americans Non-Hispanic whites are 60% ... youthonline . [Accessed 08/18/2017] HEALTH IMPACT OF OBESITY People who are overweight are more likely to ...

  9. Glaucoma in Asian Populations

    Science.gov (United States)

    ... Involved News About Us Donate In This Section Glaucoma In Asian Populations email Send this article to ... lower than in their Asian counterparts. Normal Tension Glaucoma affects Japanese Japanese populations, however, have a substantially ...

  10. Asian Australian Literatures

    OpenAIRE

    Madsen, Deborah Lea

    2007-01-01

    This article offers an overview of the range of Asian-Australian writers, within the context of changing historical and political conditions, as well as the complexity of defining a single category of literature written by Australians of Asian heritage. Such a category is difficult to define in strictly nationalistic terms as ‘Asian Australian literature’: where Australian literature is the controlling noun and ‘Asian’ functions as an adjective. Some Asian Australian writers are Australian-bo...

  11. Estimating glomerular filtration rates in elderly Chinese patients with chronic kidney disease: performance of six modified formulae developed in Asian populations.

    Science.gov (United States)

    Liu, Xun; Xu, Haixia; Zheng, Zebin; Wang, Cheng; Cheng, Cailian; Shi, Chenggang; Gong, Yihong; Li, Ming; Lou, Tanqi

    2013-01-01

    The aim of the present study was to evaluate modified glomerular filtration rate (GFR) prediction formulae in an elderly Chinese population with chronic kidney disease (CKD). A total of 378 elderly Chinese patients with CKD were enrolled. The GFR was estimated with six modified GFR prediction formulae. The performances of the estimated GFRs were compared with those of the standard GFRs measured by technetium-99m diethyl-enetraminepentaacetic acid. Biases were similar for Chinese formula 1, the Asian formula, and Chinese formula 2 (median difference, 2.22 mL/min/1.73 m(2) and 2.59 mL/min/1.73 m(2) for Chinese formula 1 and the Asian formula, respectively, versus (vs) 3.69 mL/min/1.73 m(2) for Chinese formula 2 [P = 0.298 and P = 0.913, respectively]). Precision was improved with the Japanese formula (interquartile range of the difference, 3.14 mL/min/1.73 m(2) of the Japanese formula versus 15.53-23.06 mL/min/1.73 m(2) of the other formulae). The accuracy of Chinese formula 2 was the highest (30% accuracy, 59.3% vs range 37.8-54.0% [P formulae surpassed the acceptable tolerance (>70%), and the GFR category misclassification rates for all the formulae exceeded 50%. Our findings suggest that all six modified formulae developed in Asian populations may show great bias in elderly Chinese patients with CKD. Also, our study suggests the need for uniform measures for the assessment of CKD in the elderly to guarantee better sensitivity and specificity.

  12. Utility and Usability of the Rapid Assessment of Hospital Procurement Barriers in Donation (RAPiD) as a Tool for OPO Hospital Development Staff.

    Science.gov (United States)

    Alolod, Gerard P; Traino, Heather M; Siminoff, Laura A

    2016-09-01

    Few systematic assessment tools are available to organ procurement organizations (OPOs) for evaluating donation climates of hospitals in their donation service areas (DSAs). The Rapid Assessment of hospital Procurement barriers in Donation (RAPiD) was developed for OPO hospital development staff to assess the organ donation climate of hospitals. To implement a national test of the RAPiD to examine its efficacy and usability by OPO hospital development staff. Two-arm randomized design, comparing implementation of RAPiD protocol between qualitatively trained researchers (n = 7) and OPO hospital development staff (n = 24); all evaluators received the same training assessments of high-yield hospitals. A total of 77 hospitals in DSAs of 8 OPOs. A total of 2552 health-care providers (HCPs) in high organ donor potential units. Twenty-four donation-related attitudes, knowledge, and behaviors. More HCPs interviewed in the autonomous condition were positive toward the concept of organ donation. However, HCPs in the assisted condition were more candid about and critical of the OPO. As for knowledge, fewer HCPs in the autonomous condition reported familiarity with the donation process, need for donors, and generally accepted timely referral criteria. With respect to behaviors, more respondents in the autonomous condition reported frequent or occasional contact with the OPO and routine or occasional referral criteria use. Due to issues of bias, inadequate research experience, conflicts of interest, and ongoing OPO hospital development initiatives, the RAPiD's usability by OPO-based hospital development staff is questionable and not recommended in its current form. A next generation of the RAPiD is described for future consideration. © 2016, NATCO.

  13. Asian Economic Integration Monitor November 2014

    OpenAIRE

    Asian Development Bank (ADB); Asian Development Bank (ADB); Asian Development Bank (ADB); Asian Development Bank (ADB)

    2014-01-01

    The Asian Economic Integration Monitor is a semiannual review of Asia’s regional economic cooperation and integration. It covers the 48 regional members of the Asian Development Bank. This issue includes Special Chapter: Regional Financial Integration and Crisis in Asia and Europe—A Comparative Analysis

  14. Asian Economic Integration Monitor July 2012

    OpenAIRE

    Asian Development Bank (ADB); Asian Development Bank (ADB); Asian Development Bank (ADB); Asian Development Bank (ADB)

    2012-01-01

    The Asian Economic Integration Monitor is a semiannual review of Asia’s regional economic cooperation and integration. It covers 48 regional member countries of the Asian Development Bank. This issue includes a special section—Regional Integration: A Balanced View.

  15. Development of rapid methods for relaxation time mapping and motion estimation using magnetic resonance imaging

    OpenAIRE

    Gilani, Syed Irtiza Ali

    2008-01-01

    Recent technological developments in the field of magnetic resonance imaging have resulted in advanced techniques that can reduce the total time to acquire images. For applications such as relaxation time mapping, which enables improved visualisation of in vivo structures, rapid imaging techniques are highly desirable. TAPIR is a Look- Locker-based sequence for high-resolution, multislice T1 relaxation time mapping. Despite the high accuracy and precision of TAPIR, an improveme...

  16. Development of a loop-mediated isothermal amplification method for rapid detection of pigeon circovirus.

    Science.gov (United States)

    Tsai, Shinn Shyong; Chang, Yeng Ling; Huang, Yen Li; Liu, Hung Jen; Ke, Guan Ming; Chiou, Chwei Jang; Hsieh, Yao Ching; Chang, Tsung Chou; Cheng, Li Ting; Chuang, Kuo Pin

    2014-05-01

    There are no effective antiviral treatments for pigeon circovirus (PiCV); thus, rapid diagnosis is critical for effective control of the disease caused by this virus. The recent development of a novel LAMP technique that amplifies nucleic acids rapidly with high specificity and sensitivity under isothermal conditions has overcome some of the deficiencies of nucleic-acid-based diagnostic tests. We established a LAMP method for rapid detection of PiCV using two pairs of primers that were designed from PiCV and compared its sensitivity and specificity with that of PCR. Amplification by LAMP was optimal at 63 °C for 60 min. The detection limit was nearly 0.5 pg of PiCV DNA, making it ten times more sensitive than PCR. There was no cross-reaction with porcine circovirus type 2 (PCV2), pigeon Trichomonas gallinae, or pigeon herpesvirus (PHV) under the same conditions. The assay also successfully detected the pathogen DNA in the tissues of infected pigeons. This is the first report indicating that LAMP is a valuable, rapid method of detecting PiCV with high sensitivity and specificity.

  17. Development of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food

    Science.gov (United States)

    Zhu, Longjiao; He, Jing; Cao, Xiaohan; Huang, Kunlun; Luo, Yunbo; Xu, Wentao

    2016-01-01

    Bacillus cereus is increasingly recognized as one of the major causes of food poisoning in the industrialized world. In this paper, we describe a sensitive double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) that was developed for rapid detection of B. cereus in food to minimize the risk of contamination. The polyclonal antibody (pAb) and monoclonal antibodies (mAbs) specific to B. cereus were generated from rabbit antiserum and mouse ascites, respectively, using the octanoic acid/saturated ammonium sulfate precipitation method and protein A-sepharose columns. IgG-isotype mAbs were specially developed to undergo a novel peripheral multiple sites immunization for rapid gain of hybridomas and a subtractive screen was used to eliminate cross reactivity with closely related species such as Bacillus thuringiensis, B. subtilis, B. licheniformis and B. perfringens. The linear detection range of the method was approximately 1 × 104–2.8 × 106 cells/mL with a detection limit (LOD) of 0.9 × 103 cells/mL. The assay was able to detect B. cereus when the samples were prepared in meat with various pathogens. The newly developed analytical method provides a rapid method to sensitively detect B. cereus in food specimens. PMID:26976753

  18. Evaluation of simple rapid HIV assays and development of national rapid HIV test algorithms in Dar es Salaam, Tanzania

    Directory of Open Access Journals (Sweden)

    Mbwana Judica

    2009-02-01

    Full Text Available Abstract Background Suitable algorithms based on a combination of two or more simple rapid HIV assays have been shown to have a diagnostic accuracy comparable to double enzyme-linked immunosorbent assay (ELISA or double ELISA with Western Blot strategies. The aims of this study were to evaluate the performance of five simple rapid HIV assays using whole blood samples from HIV-infected patients, pregnant women, voluntary counseling and testing attendees and blood donors, and to formulate an alternative confirmatory strategy based on rapid HIV testing algorithms suitable for use in Tanzania. Methods Five rapid HIV assays: Determine™ HIV-1/2 (Inverness Medical, SD Bioline HIV 1/2 3.0 (Standard Diagnostics Inc., First Response HIV Card 1–2.0 (PMC Medical India Pvt Ltd, HIV1/2 Stat-Pak Dipstick (Chembio Diagnostic System, Inc and Uni-Gold™ HIV-1/2 (Trinity Biotech were evaluated between June and September 2006 using 1433 whole blood samples from hospital patients, pregnant women, voluntary counseling and testing attendees and blood donors. All samples that were reactive on all or any of the five rapid assays and 10% of non-reactive samples were tested on a confirmatory Inno-Lia HIV I/II immunoblot assay (Immunogenetics. Results Three hundred and ninety samples were confirmed HIV-1 antibody positive, while 1043 were HIV negative. The sensitivity at initial testing of Determine, SD Bioline and Uni-Gold™ was 100% (95% CI; 99.1–100 while First Response and Stat-Pak had sensitivity of 99.5% (95% CI; 98.2–99.9 and 97.7% (95% CI; 95.7–98.9, respectively, which increased to 100% (95% CI; 99.1–100 on repeat testing. The initial specificity of the Uni-Gold™ assay was 100% (95% CI; 99.6–100 while specificities were 99.6% (95% CI; 99–99.9, 99.4% (95% CI; 98.8–99.7, 99.6% (95% CI; 99–99.9 and 99.8% (95% CI; 99.3–99.9 for Determine, SD Bioline, First Response and Stat-Pak assays, respectively. There was no any sample which was

  19. Estimating glomerular filtration rates in elderly Chinese patients with chronic kidney disease: performance of six modified formulae developed in Asian populations

    Directory of Open Access Journals (Sweden)

    Liu X

    2013-07-01

    tolerance (>70%, and the GFR category misclassification rates for all the formulae exceeded 50%. Conclusion: Our findings suggest that all six modified formulae developed in Asian populations may show great bias in elderly Chinese patients with CKD. Also, our study suggests the need for uniform measures for the assessment of CKD in the elderly to guarantee better sensitivity and specificity. Keywords: formula, CKD, Asian, GFR

  20. The Dust at Altitude Recovery Technology (DART) System was Developed to Recover Plant, Human, and Animal Pathogens in Asian and African Dust Storms over North America

    Science.gov (United States)

    Schuerger, A. C.; Tench, B.; Nehr, A.; Emmons, T.; Valbuena, F.; Palaia, J.; Sugars, C.

    2014-12-01

    Dust emanates year-round from Africa and Asia and impacts air quality in North America. Asian dust plumes deliver up to 64 million tonnes of dust over the NW of the USA, and African dust storms deliver over 50 million tonnes of dust over Florida each year. Several recent studies have demonstrated that human and plant pathogens from Asian [1] African [2] aerosols can be transported to N. America in naturally occurring dust storms. What is unknown is whether these 'presumptive pathogens' impact human, plant, or animal health in the USA. In order to initiate a long-term monitoring program of pathogens in Asian and African dust plumes, we have developed a dust collection system called DART (Dust at Altitude Recovery Technology) (figure). The DART dust sampler can be mounted on a F104 Starfighter jet (figure) and a T6 Texan propeller driven airplane (not shown), and was test flown over FL in Dec. 2013 on the F104 and on the T6 in the summer of 2014. The DART system utilizes a high-volume pump to pass air through 6 separate filtration units where both aerosols and microbial cells are captured. The filtration systems exhibit flow rates from 25-142 L/min depending on the pore size and brand of filters used. Flow rates are directly correlated to increased air speed, and are inversely correlated to increased altitude. Filtration units can be turned on and off individually as required for specific science flight objectives. The DART dust sampler has performed nominally up to 7600 m, 0.92 Mach, and 3.5 +G's. During initial test flights in Dec. 2013, 5 of 8 genera of fungi recovered from the lower atmosphere over FL contained plant pathogens including species in the genera: Acremonium, Aspergillus, Cladosporium, Curvularia, and Fusarium. Numbers of recovered fungi, but not bacteria, increased significantly when 5 or 10 µm filters were used in the DART system compared to filter pore sizes ≤ 1.2 µm. Future sampling programs for both Asian and African dust events will be

  1. Development of a highly sensitive lateral immunochromatographic assay for rapid detection of Vibrio parahaemolyticus.

    Science.gov (United States)

    Liu, Xinfeng; Guan, Yuyao; Cheng, Shiliang; Huang, Yidan; Yan, Qin; Zhang, Jun; Huang, Guanjun; Zheng, Jian; Liu, Tianqiang

    2016-12-01

    Vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. It is also a foodborne pathogen that causes diarrhea in humans. The aim of this study is to develop an immunochromatographic lateral flow assay (LFA) for rapid detection of V. parahaemolyticus in both aquatic products and human feces of diarrheal patients. Two monoclonal antibody (MAb) pairs, GA1a-IC9 and IC9-KB4c, were developed and proven to be highly specific and sensitive to V. parahaemolyticus. Based on the two MAb pairs, two types of LFA strips were prepared. Their testing limits for V. parahaemolyticus culture were both 1.2×103CFU/ml. The diagnostic sensitivities and specificities were both 100% for the 32 tested microbial species, including 6 Vibrio species. Subsequently, the LFA strips were used to test Whiteleg shrimps and human feces. The type II strip showed a higher diagnostic sensitivity. Its sensitivity and specificity for hepatopancreas and fecal samples from 13 Whiteleg shrimps and fecal samples from 146 human diarrheal patients were all 100%. In conclusion, our homemade type II LFA is a very promising testing device for rapid and convenient detection of V. parahaemolyticus infection not only in aquatic animals, but also in human diarrheal patients. This sensitive immunochromtographic LFA allows rapid detection of V. parahaemolyticus without requirement of culture enrichment. Copyright © 2016. Published by Elsevier B.V.

  2. The Roles of English Language Education in Asian Context

    Science.gov (United States)

    Chang, Bok-Myung

    2011-01-01

    This study surveys the history of English language and the roles of English language education in Asian context. Through the historical survey on English dispersal in Asian countries, the first section of this study traces the dispersal of English from the 18th century and the development of English in Asian countries. The second section of this…

  3. Rapidly developing multimorbidity and disability in older adults: does social background matter?

    Science.gov (United States)

    Calderón-Larrañaga, Amaia; Santoni, Giola; Wang, Hui Xin; Welmer, Anna-Karin; Rizzuto, Debora; Vetrano, Davide L; Marengoni, Alessandra; Fratiglioni, Laura

    2018-02-07

    Multimorbidity is among the most disabling geriatric conditions. In this study we explored whether a rapid development of multimorbidity potentiates its impact on the functional independence of older adults, and whether different sociodemographic factors play a role beyond the rate of chronic disease accumulation. A random sample of persons aged ≥60 years (n = 2387) from the Swedish National study on Aging and Care in Kungsholmen (SNAC-K) was followed over 6 years. The speed of multimorbidity development was estimated as the rate of chronic disease accumulation (linear mixed models) and further dichotomized into the upper versus the three lower rate quartiles. Binomial negative mixed models were used to analyse the association between speed of multimorbidity development and disability (impaired basic and instrumental activities of daily living), expressed as the incidence rate ratio (IRR). The effect of sociodemographic factors, including sex, education, occupation and social network, was investigated. The risk of new activity impairment was higher among participants who developed multimorbidity faster (IRR 2.4, 95% CI 1.9-3.1) compared with those who accumulated diseases more slowly over time, even after considering the baseline number of chronic conditions. Only female sex (IRR for women vs. men 1.6, 95% CI 1.2-2.0) and social network (IRR for poor vs. rich social network 1.7, 95% CI 1.3-2.2) showed an effect on disability beyond the rate of chronic disease accumulation. Rapidly developing multimorbidity is a negative prognostic factor for disability. However, sociodemographic factors such as sex and social network may determine older adults' reserves of functional ability, helping them to live independently despite rapid accumulation of chronic conditions. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  4. South Asian Labour and Employment Report: Promoting Inclusive ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    The majority of South Asian workers have low-paying jobs with no social protection. South Asian workers, especially women and lower caste workers, face precarious employment in the informal sector, insecure livelihoods, and lack skills. Rapid economic growth in South Asia has failed to generate good jobs for its ...

  5. Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries

    Directory of Open Access Journals (Sweden)

    Michel eDRANCOURT

    2015-11-01

    Full Text Available Culturing Mycobacterium tuberculosis remains the gold standard for the laboratory diagnosis of pulmonary tuberculosis, with 9 million new cases and 1.5 million deaths mainly in developing countries. Reviewing data reported over 20 years yields a state-of-the-art procedure for the routine culture of M. tuberculosis in both developed and developing countries. Useful specimens include sputum, induced sputum and stools collected in quaternary ammonium preservative-containing sterile cans. The usefulness of other non-invasive specimens remains to be evaluated. Specimens can be collected in a diagnosis kit also containing sampling materials, instructions, laboratory requests and informed consent. Automated direct LED fluorescence microscopy after auramine staining precedes inoculation of an egg-lecithin-containing culture solid medium under microaerophilic atmosphere, inverted microscope reading or scanning video-imaging detection of colonies and colonies identification by recent molecular methods. This procedure should result in a diagnosis of pulmonary tuberculosis as fast as 5 days. It may be implemented in both developed and developing countries with automated steps replaceable by manual steps depending on local resources.

  6. SOAR telescope control system: a rapid prototype and development in LabVIEW

    Science.gov (United States)

    Ashe, Michael C.; Schumacher, German

    2000-06-01

    A Rapid Prototype and full development plan of the SOAR TCS is reviewed to show advances in: (1) Prototyping speed, which makes implementation and test of features faster than specification under older methods. This allows the development environment and prototype modules to become partners with and part of the specification documents. (2) Real-Time performance and reliability through use of RT Linux. (3) Visually Rich GUI development that allows an emphasis on `seeing' versus `reading'. (4) Long-Term DataLogging and Internet subscription service of all desired variables with instant recall of historical trend data. (5) A `plug-in' software architecture which enables rapid reconfiguration and reuse of the system and/or plug-ins utilizing LabVIEW graphical modules, a scripting language engine (in LabVIEW) and encapsulation of interfaces in `instrument-driver' style `plug-in' modules. (6) A platform- independent development environment and distributed architecture allowing secure internet observation and control via every major OS and hardware platform.

  7. Development of Rapid Isothermal Amplification Assays for Detection of Phytophthora spp. in Plant Tissue.

    Science.gov (United States)

    Miles, Timothy D; Martin, Frank N; Coffey, Michael D

    2015-02-01

    Several isothermal amplification techniques recently have been developed that are tolerant of inhibitors present in many plant extracts, which can reduce the need for obtaining purified DNA for running diagnostic assays. One such commercially available technique that has similarities with real-time polymerase chain reaction (PCR) for designing primers and a labeled probe is recombinase polymerase amplification (RPA). This technology was used to develop two simple and rapid approaches for detection of Phytophthora spp.: one genus-specific assay multiplexed with a plant internal control and the other species-specific assays for Phytophthora ramorum and P. kernoviae. All assays were tested for sensitivity (ranging from 3 ng to 1 fg of DNA) and specificity using DNA extracted from more than 136 Phytophthora taxa, 21 Pythium spp., 1 Phytopythium sp., and a wide range of plant species. The lower limit of linear detection using purified DNA was 200 to 300 fg of DNA in all pathogen RPA assays. Six different extraction buffers were tested for use during plant tissue maceration and the assays were validated in the field by collecting 222 symptomatic plant samples from over 50 different hosts. Only 56 samples were culture positive for Phytophthora spp. whereas 91 were positive using the Phytophthora genus-specific RPA test and a TaqMan real-time PCR assay. A technique for the generation of sequencing templates from positive RPA amplifications to confirm species identification was also developed. These RPA assays have added benefits over traditional technologies because they are rapid (results can be obtained in as little as 15 min), do not require DNA extraction or extensive training to complete, use less expensive portable equipment than PCR-based assays, and are significantly more specific than current immunologically based methods. This should provide a rapid, field-deployable capability for pathogen detection that will facilitate point-of-sample collection processing

  8. Identification of pollutant sources in a rapidly developing urban river catchment in China

    Science.gov (United States)

    Huang, Jingshui; Yin, Hailong; Jomma, Seifeddine; Rode, Michael; Zhou, Qi

    2016-04-01

    Rapid economic development and urbanization worldwide cause serious ecological and environmental problems. A typical region that is in transition and requires systemic research for effective intervention is the rapidly developing city of Hefei in central P. R. China. In order to investigate the sources of pollutants over a one-year period in Nanfei River catchment that drains the city of Hefei, discharges were measured and water samples were taken and measured along the 14km river section at 10 sites for 4 times from 2013 to 2014. Overflow concentrations of combined sewer and separate storm drains were also measured by selecting 15 rain events in 4 typical drainage systems. Loads and budgets of water and different pollutant sources i.e., wastewater treatment plant (WWTP) effluent, urban drainage overflow, unknown wastewater were calculated. The water balance demonstrated that >70% of the discharge originated from WWTP effluent. Lack of clean upstream inflow thereby is threatening ecological safety and water quality. Furthermore, mass fluxes calculations revealed that >40% of the COD (Chemical Oxygen Demand) loads were from urban drainage overflow because of a large amount of discharge of untreated wastewater in pumping stations during rain events. WWTP effluent was the predominant source of the total nitrogen loads (>60%) and ammonia loads (>45%). However, the total phosphorous loads from three different sources are similar (˜1/3). Thus, our research provided a basis for appropriate and prior mitigation strategies (state-of-art of WWTP upgrade, sewer systems modification, storm water regulation and storage capacity improvement, etc.) for different precedence-controlled pollutants with the limited infrastructure investments in these rapidly developing urban regions.

  9. DEVELOPMENT OF RAPID TECHNIQUE FOR DETERMINATION OF THE TOTAL MINERALIZATION OF NATURAL WATERS

    Directory of Open Access Journals (Sweden)

    T. A. Kuchmenko

    2015-01-01

    Full Text Available A new approach has been proposed for rapid and easy evaluation of a indicator of quality and properties of natural water - soluble salt content (mineralization. The method of quartz crystal microbalance is employed at load of the mass-sensitive resonator electrode (BAW-type with investigated water. The degree of correlation between the various indicators related to the contents of salts and insoluble compounds and the level of mineralization obtained by the standard method (gravimetry has been studied. A procedure for salt weighing by single sensor at unilateral load with small sample of natural water has been developed. The optimal conditions for measurement is established using the design of experiment by model 23 . The possibilities of quartz crystal microbalance for determination of non-volatile compounds in the water are described. The calibration of piezosensor is produced by standard solution NaCl (c = 1.000 g / dm3 at optimal conditions of experiment. The adequacy and accuracy of proposed technique is assessed by the correlation between the results of quartz crystal microbalance and conductometry. The correlation between indicators of mineralization established by quartz crystal microbalance and gravimetry is found. It has been obtained an equation that can be used to calculate the standard indicator of the mineralization by the results of a quartz crystal microbalance using single sensor. The approaches to enhance the analytical capabilities of the developed technique for water with low and high mineralization are proposed. The metrological characteristics of quartz crystal microbalance of insoluble compounds in natural water are estimated. A new technique of determination of the mass concentration of the dry residue in water with a conductivity of 0.2 mS or above has been developed, which can be used for rapid analysis of the water at nonlaboratory conditions and in the laboratory for rapid obtaining the information about a sample.

  10. Bordetella trematum sepsis with shock in a diabetic patient with rapidly developing soft tissue infection.

    Science.gov (United States)

    Majewski, Lorrance L; Nogi, Masayuki; Bankowski, Matthew J; Chung, Heath H

    2016-09-01

    Bordetella is a gram-negative, glucose non-fermenting bacillus, consisting of many host-associated species. B. trematum has previously been identified in wound infections, but rarely known to be a source of bacteremia. Currently, 16S rRNA sequencing represents the reference standard method by which identification is made. Herein, we present a case of fatal B. trematum bacteremia with septic shock. The presumed primary site of the infection was a rapidly developing left leg deep soft tissue infection without necrotizing fasciitis. B. trematum should now be considered as a significant pathogen in sepsis. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. RAPID NAMING IN CHILDREN WITH SPECIFIC LANGUAGE IMPAIRMENT AND IN CHILDREN WITH TYPICAL LANGUAGE DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    Neda MILOSHEVIĆ

    2017-03-01

    Full Text Available Aimed at the detailed insight into the phonological ability of Serbian-speaking children of preschool age, with and without language impairment, the ability of rapid naming was examined. Method: Operationalization of the set goal was carried out by using the Test for evaluating reading and writing pre-skills. In describing and analyzing the obtained data, methods of descriptive and inferential statistics were used. The sample included 120 subjects of both gender, 40 children diagnosed with specific language impairment (SLI, age from 5,11 to 7 years, and 80 children with typical language development (TLD, age between 5,11 and 7 years, with no statistically significant differences in relation to age and gender of the participants. Results: Summing up the overall results and achievements of children with SLI and children with TLD, we concluded that there are statistically significant differences in the rapid naming between children with specific language impairment and children with typical language development. Conclusions: As it is a global trend to work on preventing disorders and obstructions, and phonological skills in this age are a timely indicator of the development of reading and writing skills, the examined children with SLI are at risk for the occurrence of obstructions and disorders in the area of reading and writing abilities.

  12. Tracking Change in rapid and eXtreme Development: A Challenge to SCM-tools?

    DEFF Research Database (Denmark)

    Christensen, Henrik Bærbak

    2001-01-01

    Software configuration management (SCM) has proved to be an invaluable part of developing and maintaining high quality software. The benefits are not for free however: SCM tool operations often divert your attention from your development task, sometimes you have to endure a long waiting time while...... the tool does its job, change descriptions must be memorised until your next check-in, etc. This kind of overhead and disruption does not fit well with fast-paced development processes like rapid prototyping, explorative programming, and eXtreme Programming that favour creativity, speed, and communication...... more that managerial rigour. In the cost/benefit equation the balance may tip in favour of not using any SCM tool or only using a fraction of its potential. We think SCM has something to offer such projects, and present some proposals that may allow SCM tools to better suit the characteristics of fast...

  13. Development and validation of a real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay for investigation of wild poliovirus type 1-South Asian (SOAS) strain reintroduced into Israel, 2013 to 2014.

    Science.gov (United States)

    Hindiyeh, M Y; Moran-Gilad, J; Manor, Y; Ram, D; Shulman, L M; Sofer, D; Mendelson, E

    2014-02-20

    In February 2013, wild poliovirus type 1 (WPV1) was reintroduced into southern Israel and resulted in continuous silent circulation in the highly immune population. As a part of the public health emergency response, a novel real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed, to allow for the sensitive and specific detection of the circulatingWPV1-South Asian (SOAS) strain. Specific primers and probes derived from the VP-1 region were designed, based on sequenced sewage isolates, and used to simultaneously amplify this WPV1-SOAS sequence together with bacteriophage MS-2 as internal control. High titre WPV1-SOAS stock virus was used for assay optimisation and 50 processed sewage samples collected from southern Israel and tested by reference culture based methods were used for analytical validation of the assay’s performance. The limit of detection of the multiplex qRT-PCR (SOAS/MS-2) assay was 0.1 plaque-forming unit (pfu)/reaction (20 pfu/mL) for WPV1-SOAS RNA with 100% sensitivity, specificity, positive and negative predictive values when compared to the culture based method. The turnaround time was rapid, providing results for environmental samples within 24 to 48 hours from completion of sewage processing, instead of five to seven days by culture-based analysis. Direct sewage testing by qRT-PCR assay proved to be a useful tool for rapid detection and environmental surveillance of WPV1-SOAS circulating strain during emergency response. Application of the approach for detection of WPV1-SOAS in stool samples obtained during acute flaccid paralysis (AFP) surveillance or field surveys should be further evaluated.

  14. The Water-Energy-Food Nexus in a Rapidly Developing Resource Sector

    Science.gov (United States)

    Allen, D. M.; Kirste, D. M.

    2014-12-01

    Technological advances and access to global markets have changed the rate at which resource exploitation takes place. The environmental impact of the rapid development and distribution of resources such as minerals and hydrocarbons has led to a greater potential for significant stress on water resources both in terms of quality and quantity. How and where those impacts manifest is crucial to determining appropriate risk management strategies. North East British Columbia has an abundance of shale gas reserves that are anticipated to be exploited at a large scale in coming years, primarily for export as liquefied natural gas (LNG). However, there is growing concern that fracking and other activities related to shale gas development pose risks to water quality and quantity in the region. Water lies at the center of the water-energy-food nexus, with an accelerating water demand for fracking and industrial operations as well as for domestic, environmental and agricultural uses. Climate change is also anticipated to alter the hydrologic regime, posing added stress to the water resource. This case study examines the water-energy-food nexus in the context of a region that is impacted by a rapidly developing resource sector, encompassing water demand/supply, climate change, interaction between deep aquifers and shallow aquifers/surface waters, water quality concerns related to fracking, land use disturbance, and community impacts. Due to the rapid rate of development, there are significant knowledge gaps in our understanding of the water resource. Currently agencies are undertaking water resource assessments and establishing monitoring sites. This research aims to assess water security in North East British Columbia in a coordinated fashion through various partnerships. In addition to collecting baseline knowledge and data, the study will evaluate risk and resilience indicators in relation to water security. A risk assessment framework specific to the shale gas development

  15. The complex role of social care services in supporting the development of sustainable identities: Insights from the experiences of British South Asian women with intellectual disabilities.

    Science.gov (United States)

    Malik, Kulsoom Jawaid; Unwin, Gemma; Larkin, Michael; Kroese, Biza Stenfert; Rose, John

    2017-04-01

    Carers and service users with intellectual disabilities from minority ethnic groups have typically been reported to be dissatisfied with the social care services they receive. However, service users themselves have rarely been asked directly about their experiences of social care. This paper aims to understand the meaning of social care services in the lives of South Asian women with intellectual disabilities, in the United Kingdom. 10 British South Asian women with mild-moderate intellectual disabilities were interviewed about their experiences of social care services. The transcripts were analysed using interpretative phenomenological analysis. The analysis produced three super-ordinate themes, which focus on how services facilitate the development of complex identities, how the participants explored their sense of being 'stuck' between cultures as they negotiated their journeys towards independence, and the triple disadvantage which they experienced as a consequence of the intersection between gender, ethnicity and disability. The participants were broadly satisfied with the role which services played in these domains, and appeared to find them valuable and helpful. The results suggest that the participants successfully managed complex identity issues, such as acculturation processes, with the support of services. It may be helpful to give more explicit consideration to the positive role which good services can play in supporting people with intellectual disabilities in the development of their identities and goals, alongside the more traditionally 'concrete' objectives of such social care. Engagement with families in 'positive risk-taking' is likely to be an important component of success. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Synthesis report on rearing Asian longhorned beetle

    Science.gov (United States)

    Melody A. Keena; Ann E. Hajek; Thomas L. M. Dubois; David R. Lance

    2003-01-01

    Since not all research on Anoplophora glabripennis (Motschulsky) (ALB) can be conducted in China or at North American sites where it is being eradicated, the ability to mass rear the Asian longhorned beetle is critical to rapid progress on research necessary for exclusion, detection, and eradication of this serious pest.

  17. Non-Obese Diabetic Mice Rapidly Develop Dramatic Sympathetic Neuritic Dystrophy

    Science.gov (United States)

    Schmidt, Robert E.; Dorsey, Denise A.; Beaudet, Lucie N.; Frederick, Kathy E.; Parvin, Curtis A.; Plurad, Santiago B.; Levisetti, Matteo G.

    2003-01-01

    To address the pathogenesis of diabetic autonomic neuropathy, we have examined the sympathetic nervous system in non-obese diabetic (NOD) and streptozotocin (STZ)-induced diabetic mice, two models of type 1 diabetes, and the db/db mouse, a model of type 2 diabetes. After only 3 to 5 weeks of diabetes, NOD mice developed markedly swollen axons and dendrites (“neuritic dystrophy”) in the prevertebral superior mesenteric and celiac ganglia (SMG-CG), similar to the pathology described in diabetic STZ- and BBW-rat and man. Comparable changes failed to develop in the superior cervical ganglia of the NOD mouse or in the SMG-CG of non-diabetic NOD siblings. STZ-induced diabetic mice develop identical changes, although at a much slower pace and to a lesser degree than NOD mice. NOD-SCID mice, which are genetically identical to NOD mice except for the absence of T and B cells, do not develop diabetes or neuropathology comparable to diabetic NOD mice. However, STZ-treated NOD-SCID mice develop severe neuritic dystrophy, evidence against an exclusively autoimmune pathogenesis for autonomic neuropathy in this model. Chronically diabetic type 2 db/db mice fail to develop neuritic dystrophy, suggesting that hyperglycemia alone may not be the critical and sufficient element. The NOD mouse appears to be a valuable model of diabetic sympathetic autonomic neuropathy with unambiguous, rapidly developing neuropathology which corresponds closely to the characteristic pathology of other rodent models and man. PMID:14578206

  18. Old knowledge and new technologies allow rapid development of model organisms.

    Science.gov (United States)

    Cook, Charles E; Chenevert, Janet; Larsson, Tomas A; Arendt, Detlev; Houliston, Evelyn; Lénárt, Péter

    2016-03-15

    Until recently the set of "model" species used commonly for cell biology was limited to a small number of well-understood organisms, and developing a new model was prohibitively expensive or time-consuming. With the current rapid advances in technology, in particular low-cost high-throughput sequencing, it is now possible to develop molecular resources fairly rapidly. Wider sampling of biological diversity can only accelerate progress in addressing cellular mechanisms and shed light on how they are adapted to varied physiological contexts. Here we illustrate how historical knowledge and new technologies can reveal the potential of nonconventional organisms, and we suggest guidelines for selecting new experimental models. We also present examples of nonstandard marine metazoan model species that have made important contributions to our understanding of biological processes. © 2016 Cook et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  19. Development of a Recombinase Polymerase Amplification Assay for Rapid Detection of the Mycobacterium avium subsp. paratuberculosis.

    Directory of Open Access Journals (Sweden)

    Sören Hansen

    Full Text Available The detection of Mycobacterium avium subsp. paratuberculosis (MAP infections in ruminants is crucial to control spread among animals and to humans. Cultivation of MAP is seen as the gold standard for detection, although it is very time consuming and labour intensive. In addition, several PCR assays have been developed to detect MAP in around 90 minutes, but these assays required highly sophisticated equipment as well as lengthy and complicated procedure.In this study, we have developed a rapid assay for the detection of MAP based on the recombinase polymerase amplification (RPA assay targeting a MAP specific region, the IS900 gene. The detection limit was 16 DNA molecules in 15 minutes as determined by the probit analysis on eight runs of the plasmid standard. Cross reactivity with other mycobacterial and environmentally associated bacterial strains was not observed. The clinical performance of the MAP RPA assay was tested using 48 MAP-positive and 20 MAP-negative blood, sperm, faecal and tissue samples. All results were compared with reads of a highly sensitive real-time PCR assay. The specificity of the MAP RPA assay was 100%, while the sensitivity was 89.5%.The RPA assay is quicker and much easier to handle than real-time PCR. All RPA reagents were cold-chain independent. Moreover, combining RPA assay with a simple extraction protocol will maximize its use at point of need for rapid detection of MAP.

  20. Development of a lateral flow immunochromatographic assay for the rapid diagnosis of Orf virus infections.

    Science.gov (United States)

    Zhao, Kui; He, Wenqi; Bi, Jingying; Zhang, Ximu; Zhang, Di; Huang, Houshuang; Zhang, Yuexiang; Song, Deguang; Gao, Feng

    2016-10-01

    A rapid and simple lateral-flow immunochromatographic assay (LFIA) was developed for the specific detection of Orf virus (ORFV) using two distinct monoclonal antibodies (MAbs: 5A5 and 6F2) against the ORFV ORF011 protein. The MAb 5A5 was conjugated with colloidal gold, and the MAb 6F2 and goat anti-mouse IgG were sprayed onto a nitrocellulose membrane in strips at positions designated test (T) and control (C), respectively. The results showed that samples of ORFV complexed with colloidal gold-conjugated MAb 5A5, were captured by MAb 6F2 at the T line resulting in the appearance of a purple band. When samples did not contain ORFV or when they contained a quantity of ORFV below the detection limit of the test, only the C line was visible. The analysis of sensitivity of the test demonstrated that the lowest detected quantity of ORFV was 2.03×10(3.0) TCID50/ml. Storage at room temperature for 6 months did not result in the loss of performance of the LFIA test. Using loop-mediated isothermal amplification (LAMP) as a reference test, the relative specificity and sensitivity of the LFIA test were determined to be 100% and 92.1%, respectively. Based on these results, the LFIA test developed may be a suitable tool for rapid on-site testing for ORFV infection. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Development of a Rapid Beam Emittance Measurement System using a Real-Time Beam Profile Monitor

    Science.gov (United States)

    Kamakura, Keita; Hatanaka, Kichiji; Fukuda, Mitsuhiro; Yorita, Tetsuhiko; Ueda, Hiroshi; Saito, Takane; Morinobu, Shunpei; Nagayama, Keiichi; Tamura, Hitoshi; Yasuda, Yuusuke

    2016-06-01

    We have developed a rapid beam emittance measurement system for the injection beam of the K140 azimuthally varying field (AVF) cyclotron at Research Center for Nuclear Physics (RCNP). So far, a conventional emittance monitor has been used in a section of a medium energy beam transport (MEBT) system to evaluate the quality of the injected beam to the K400 ring cyclotron. Two kinds of emittance monitors were supplemented in the low energy beam line for evaluation of ion beams from ion sources. One of them is a conventional type consisting of two sets of position-variable slits and a three-wire profile monitor (TPM), similar to the one installed in the MEBT system of the AVF cyclotron. It takes about 30 min to get emittances in both the horizontal and vertical planes. For quick emittance measurements, we have developed a new system equipped with a set of fast moving slits with a fixed gap and a real-time beam profile monitor (BPM83) with a rotating helical wire. With this system the measurement time was considerably reduced to 70 s for both the horizontal and vertical emittances. Moreover the data analysis and graphical processing were completely automated. The overall measurement and analysis time was successfully minimized within 75 s. This rapid emittance measurement system has contributed to improve the beam quality by optimizing parameters of ion sources and the beam transport system.

  2. Professional Development Needs of Novice Nursing Clinical Teachers: A Rapid Evidence Assessment.

    Science.gov (United States)

    Jetha, Farah; Boschma, Geertje; Clauson, Marion

    2016-02-26

    The current nursing profession is challenged with a decreasing supply of competent clinical teachers due to several factors consequently impacting the quality of nursing education. To meet this demand, academic nursing programs are resorting to hiring expert nurses who may have little or no teaching experience. They need support during their transition from practice to teaching. Using the systematic approach of a Rapid Evidence Assessment (REA), scholarly literature was reviewed to identify existing professional development needs for novice clinical teachers as well as supportive strategies to aid the transition of experienced nurses into teaching practice. The REA included 29 relevant studies. Findings revealed three main professional development needs for novice clinical teachers and key supportive strategies. Based on these findings recommendations for best practices to support and prepare novice clinical teachers are presented.

  3. Rapid crown root development confers tolerance to zinc deficiency in rice

    Directory of Open Access Journals (Sweden)

    Amrit Kaur eNanda

    2016-03-01

    Full Text Available Zinc (Zn deficiency is one of the leading nutrient disorders in rice (Oryza sativa. Many studies have identified Zn efficient rice genotypes, but causal mechanisms for Zn deficiency tolerance remain poorly understood. Here we report a detailed study of the impact of Zn deficiency on crown root development of rice genotypes, differing in their tolerance to this stress. Zn deficiency delayed crown root development and plant biomass accumulation in both Zn efficient and inefficient genotypes, with the effects being much stronger in the latter. Zn efficient genotypes had developed new crown roots as early as three days after transplanting (DAT to a Zn deficient field and that was followed by a significant increase in total biomass by 7 DAT. Zn-inefficient genotypes developed few new crown roots and did not increase biomass during the first seven days following transplanting. This correlated with Zn efficient genotypes retranslocating a higher proportion of shoot Zn to their roots, compared to Zn inefficient genotypes. These latter genotypes were furthermore not efficient in utilizing the limited Zn for root development. Histological analyses indicated no anomalies in crown tissue of Zn-efficient or inefficient genotypes that would have suggested crown root emergence was impeded. We therefore conclude that the rate of crown root initiation was differentially affected by Zn deficiency between genotypes. Rapid crown root development, following transplanting, was identified as a main causative trait for tolerance to Zn deficiency and better Zn retranslocation from shoot to root was a key attribute of Zn-efficient genotypes.

  4. Development of a lateral flow immunoassay for rapid diagnosis of potato blackleg caused by Dickeya species.

    Science.gov (United States)

    Safenkova, Irina V; Zaitsev, Ilya A; Varitsev, Yuri A; Byzova, Nadezhda A; Drenova, Natalia V; Zherdev, Anatoly V; Dzantiev, Boris B

    2017-03-01

    Early detection of potato infections is essential for effective disease management. The aim of this study was to develop a lateral flow immunoassay (LFIA) for rapid detection of a serious potato disease, potato blackleg, caused by Dickeya dianthicola and Dickeya solani. Polyclonal antibodies specific to different strains of Dickeya were obtained from rabbits after immunization with bacterial cells of D. dianthicola and D. solani. Enzyme-linked immunosorbent assay testing with use of a wide range of bacterial species showed that the polyclonal antibodies detect closely related strains of D. dianthicola and D. solani. Cross-reactivity with widespread pathogenic bacteria (nine species) and saprophytes of healthy potato plants was not detected. The LFIA based on the obtained antibodies and gold nanoparticles with average diameter of 20 nm was developed. Under optimized conditions, the LFIA method enabled the analysis of potato extracts within 10 min, with a visual limit of detection of 1 × 10 5  CFU/ml for leaves and 4 × 10 5  CFU/ml for tubers. The assay was tested on potato stem and tuber extracts, and the results of the LFIA were confirmed in 92.1% of samples using the real-time polymerase chain reaction. The findings confirmed that the developed LFIA could be used for monitoring blackleg infection without the need for special equipment or skills. Graphical Abstract The developed lateral flow immunoassay is an efficient tool for rapid detection of a serious potato disease, potato blackleg, caused by Dickeya dianthicola and Dickeya solani.

  5. Development and Validation of a Lateral Flow Immunoassay for Rapid Detection of NDM-Producing Enterobacteriaceae.

    Science.gov (United States)

    Boutal, Hervé; Naas, Thierry; Devilliers, Karine; Oueslati, Saoussen; Dortet, Laurent; Bernabeu, Sandrine; Simon, Stéphanie; Volland, Hervé

    2017-07-01

    The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flow immunoassay (LFIA) (called the NDM LFIA) for the rapid and reliable detection of NDM-like carbapenemase-producing Enterobacteriaceae from culture colonies. We evaluated the NDM LFIA using 175 reference enterobacterial isolates with characterized β-lactamase gene content and 74 nonduplicate consecutive carbapenem-resistant clinical isolates referred for expertise to the French National Reference Center (NRC) for Antibiotic Resistance during a 1-week period (in June 2016). The reference collection included 55 non-carbapenemase producers and 120 carbapenemase producers, including 27 NDM producers. All 27 NDM-like carbapenemase producers of the reference collection were correctly detected in less than 15 min by the NDM LFIA, including 22 strains producing NDM-1, 2 producing NDM-4, 1 producing NDM-5, 1 producing NDM-7, and 1 producing NDM-9. All non-NDM-1 producers gave a negative result with the NDM LFIA. No cross-reaction was observed with carbapenemases (VIM, IMP, NDM, KPC, and OXA-48-like), extended-spectrum β-lactamases (ESBLs) (TEM, SHV, and CTX-M), AmpCs (CMY-2, DHA-2, and ACC-1), and oxacillinases (OXA-1, -2, -9, and -10). Similarly, among the 74 referred nonduplicate consecutive clinical isolates, all 7 NDM-like producers were identified. Overall, the sensitivity and specificity of the assay were 100% for NDM-like carbapenemase detection with strains cultured on agar. The NDM LFIA was efficient, rapid, and easy to implement in the routine workflow of a clinical microbiology laboratory for the confirmation of NDM-like carbapenemase-producing Enterobacteriaceae . Copyright © 2017 Boutal et al.

  6. Development of a rapid dipstick with latex immunochromatographic assay (DLIA for diagnosis of schistosomiasis japonica

    Directory of Open Access Journals (Sweden)

    Lu Shao-Hong

    2011-08-01

    Full Text Available Abstract Background Schistosomiasis japonica (schistosomiasis is a zoonosis that can seriously affect human health. At present, the immunodiagnostic assays for schistosomiasis detection are time-consuming and require well-trained personnel and special instruments, which can limit their use in the field. Thus, there is a pressing need for a simple and rapid immunoassay to screen patients on a large scale. In this study, we developed a novel rapid dipstick with latex immunochromatographic assay (DLIA to detect anti-Schisaosoma japonicum antibodies in human serum. Results Using latex microspheres as a color probe, DLIA was established to test standard positive and negative sera, in comparison with the classical enzyme-linked immunosorbent assay (ELISA. The sensitivity and specificity of DLIA were 95.10% (97/102 and 94.91% (261/275, respectively. The cross-reaction rates with clonorchiosis, intestinal nematodes, Angiostrongylus cantonensis and paragonimiasis were 0, 0, 0 and 42.11% respectively. All the results showed no significant difference to the ELISA. In field tests, 333 human serum samples from an endemic area were tested with DLIA, and compared with ELISA and Kato-Katz method. There was no significant difference between DLIA and ELISA on positive and negative rates of detection; however, significant differences existed between DLIA and Kato-Katz method, and between ELISA and Kato-Katz method. The kappa value between DLIA and ELISA was 0.90. Conclusions This is the first study in which DLIA was used to detect anti-Schistosoma japonicum antibody. The results show that DLIA is a simple, rapid, convenient, sensitive and specific assay for the diagnosis of schistosomiasis and is therefore very suitable for large-scale field applications and clinical detection.

  7. Predictive Management of Asian Carps in the Upper Mississippi River System

    Science.gov (United States)

    Vondracek, Bruce C.; Carlson, Andrew K.

    2014-01-01

    Prolific non-native organisms pose serious threats to ecosystems and economies worldwide. Nonnative bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix), collectively referred to as Asian carps, continue to colonize aquatic ecosystems throughout the central United States. These species are r-selected, exhibiting iteroparous spawning, rapid growth, broad environmental tolerance, high density, and long-distance movement. Hydrological, thermal, and physicochemical conditions are favorable for establishment beyond the current range, rendering containment and control imperative. Ecological approaches to confine Asian carp populations and prevent colonization characterize contemporary management in the United States. Foraging and reproduction of Asian carps govern habitat selection and movement, providing valuable insight for predictive control. Current management approaches are progressive and often anticipatory but deficient in human dimensions. We define predictive management of Asian carps as synthesis of ecology and human dimensions at regional and local scales to develop strategies for containment and control. We illustrate predictive management in the Upper Mississippi River System and suggest resource managers integrate predictive models, containment paradigms, and human dimensions to design effective, socially acceptable management strategies. Through continued research, university-agency collaboration, and public engagement, predictive management of Asian carps is an auspicious paradigm for preventing and alleviating consequences of colonization in the United States.

  8. Osteoporosis and Asian American Women

    Science.gov (United States)

    ... Breadcrumb Home Osteoporosis Osteoporosis and Asian American Women Osteoporosis and Asian American Women Asian American women are ... Are Available? Resources For Your Information What Is Osteoporosis? Osteoporosis is a condition in which the bones ...

  9. Penerapan Aplikasi Program Penjualan Dan Pembelian Menggunakan Model Rapid Application Development

    Directory of Open Access Journals (Sweden)

    Annisa Febriani

    2017-09-01

    Abstract The development of information technology at the moment quickly and rapidly, supported by one means namely computer. Of course the computer has been equipped with a particular application is used to help facilitate the work of the man to manage the data of an organization or company so that getting accurate results and according to needs. The results of the observations that have been made, showed a sales and purchase activities are still using manual systems, one of them at a clothing store. Starting from the data processing of the goods, the difficulty of checking stock, purchase transaction, sales transactions, as well as other data storage associated with all types of such activities, so that it could make a loss for the store owner, errors in the logging and less akuratnya the report is made. Judging from the large number of transactions done on clothing stores, required system information more quickly and accurately. Thus, the author makes the program architecture-based computer, use the Microsoft Visual Basic.net programming language and the MySQL database, so that the information and activities that occur can be done quickly and accurately. The methods used in making architecture the program using the model of Rapid Application Development (RAD. This RAD model is an adaptation of the waterfall model for high speed version of the development of each component of its software. Results achieved from the discussion of this theme is the form of the application program selling and buying the ready-made. In this case, the use of the application program is the best solution to solve the existing problems, as well as with the use of application programs can be reached by an activity which is effective and efficient in supporting that activity, especially for addressing the problem of the sale and purchase of.   Keywords: Sales Program, Purchasing Program.

  10. Clustering of smoking, alcohol drinking and cannabis use in adolescents in a rapidly developing country

    Directory of Open Access Journals (Sweden)

    Chiolero Arnaud

    2006-06-01

    Full Text Available Abstract Background Smoking, alcohol drinking and cannabis use ("risk behaviors" are often initiated at a young age but few epidemiological studies have assessed their joined prevalence in children in developing countries. This study aims at examining the joint prevalence of these behaviors in adolescents in the Seychelles, a rapidly developing country in the Indian Ocean. Methods Cross-sectional survey in a representative sample of secondary school students using an anonymous self-administered questionnaire (Global Youth Tobacco Survey. The questionnaire was completed by 1,321 (92% of 1,442 eligible students aged 11 to 17 years. Main variables of interest included smoking cigarettes on ≥1 day in the past 30 days; drinking any alcohol beverage on ≥1 day in the past 30 days and using cannabis at least once in the past 12 months. Results In boys and girls, respectively, prevalence (95% CI was 30% (26–34/21% (18–25 for smoking, 49% (45–54/48% (43–52 for drinking, and 17% (15–20/8% (6–10 for cannabis use. The prevalence of all these behaviors increased with age. Smokers were two times more likely than non-smokers to drink and nine times more likely to use cannabis. Drinkers were three times more likely than non-drinkers to smoke or to use cannabis. Comparison of observed versus expected frequencies of combination categories demonstrated clustering of these risk behaviors in students (P Conclusion Smoking, drinking and cannabis use were common and clustered among adolescents of a rapidly developing country. These findings stress the need for early and integrated prevention programs.

  11. Development of field-based separations for the rapid identification of uranium and plutonium

    Energy Technology Data Exchange (ETDEWEB)

    Mertz, Carol J.; Kaminski, Michael D.; Shkrob, Ilya A.; Kalensky, Michael; Sullivan, Vivian S.; Tsai, Yifen

    2015-05-08

    The development of rapid, radioanalytical techniques to separate uranium and plutonium from complex, field samples are needed for the timely and accurate determination of nuclear material origin, and processing activities. Widespread use of nuclear power and technology in the world has increased demands on analytical laboratories from the monitoring of numerous low-level, environmental samples with variable compositions. Environmental sampling has proven to be one of the strongest technical measures for detecting nuclear material and activities. With the increase in sampling demands, new technologies must offer improvements such as automation, high throughput, reproducible chemical separations, short analysis times, and reduced costs to be effective. We have been developing a portable, separations system for uranium (U) and plutonium (Pu) separations based upon selective extraction of target elements using an extraction chromatographic resin which would allow for simple and fast identifcation when coupled with the appropriate sample digestor and detection systems. The microfluidic design minimizes elution volumes and concentrates the elements of interest in a purified stream. Flowsheet development and testing was demonstrated on a single, micro-column system with an acidified, iron, uranium, and plutonium nitrate stream. The recovery of Pu was optimized by examining various reducing agents at different concentrations for rapid, quantitative recovery from the flow-through design. Quantitative recovery and high selectivity of U and Pu was achieved in the appropriate stripping stages and provided purified and concentrated U and Pu streams. The microfluidic system suggests automation in a small, footprint unit while exploiting the in-line processing of extraction chromatographic resins as the primary means of concentrating the radionuclides from the raw acidic feed and separating the elements into purified streams.

  12. Development of a Rapid Salivary Proteomic Platform for Oral Feeding Readiness in the Preterm Newborn

    Directory of Open Access Journals (Sweden)

    Prarthana Khanna

    2017-12-01

    Full Text Available Oral feeding competency is a major determinant of length of stay in the neonatal intensive care unit. An infant must be able to consistently demonstrate the ability to take all required enteral nutrition by mouth before discharge home. Most infants born prematurely (<37 weeks will require days, if not weeks, to master this oral feeding competency skill. Inappropriately timed feeding attempts can lead to acute and long-term morbidities, prolonged hospitalizations, and increased health-care costs. Previously, a panel of five genes involved in essential developmental pathways including sensory integration (nephronophthisis 4, Plexin A1, hunger signaling [neuropeptide Y2 receptor (NPY2R, adenosine-monophosphate-activated protein kinase (AMPK], and facial development (wingless-type MMTV integration site family, member 3 required for oral feeding success were identified in neonatal saliva. This study aimed to translate these five transcriptomic biomarkers into a rapid proteomic platform to provide objective, real-time assessment of oral feeding skills, to better inform care, and to improve neonatal outcomes. Total protein was extracted from saliva of 10 feeding-successful and 10 feeding-unsuccessful infants matched for age, sex, and post-conceptional age. Development of immunoassays was attempted for five oral feeding biomarkers and two reference biomarkers (GAPDH and YWHAZ to normalize for starting protein concentrations. Normalized protein concentrations were correlated to both feeding status at time of sample collection and previously described gene expression profiles. Only the reference proteins and those involved in hunger signaling were detected in neonatal saliva at measurable levels. Expression patterns for NPY2R and AMPK correlated with the gene expression patterns previously seen between successful and unsuccessful feeders and predicted feeding outcome. Salivary proteins associated with hunger signaling are readily quantifiable in

  13. Controlled precipitation for enhanced dissolution rate of flurbiprofen: development of rapidly disintegrating tablets.

    Science.gov (United States)

    Essa, Ebtessam A; Elmarakby, Amira O; Donia, Ahmed M A; El Maghraby, Gamal M

    2017-09-01

    The aim of this work was to investigate the potential of controlled precipitation of flurbiprofen on solid surface, in the presence or absence of hydrophilic polymers, as a tool for enhanced dissolution rate of the drug. The work was extended to develop rapidly disintegrated tablets. This strategy provides simple technique for dissolution enhancement of slowly dissolving drugs with high scaling up potential. Aerosil was dispersed in ethanolic solution of flurbiprofen in the presence and absence of hydrophilic polymers. Acidified water was added as antisolvent to produce controlled precipitation. The resultant particles were centrifuged and dried at ambient temperature before monitoring the dissolution pattern. The particles were also subjected to FTIR spectroscopic, X-ray diffraction and thermal analyses. The FTIR spectroscopy excluded any interaction between flurbiprofen and excipients. The thermal analysis reflected possible change in the crystalline structure and or crystal size of the drug after controlled precipitation in the presence of hydrophilic polymers. This was further confirmed by X-ray diffraction. The modulation in the crystalline structure and size was associated with a significant enhancement in the dissolution rate of flurbiprofen. Optimum formulations were successfully formulated as rapidly disintegrating tablet with subsequent fast dissolution. Precipitation on a large solid surface area is a promising strategy for enhanced dissolution rate with the presence of hydrophilic polymers during precipitation process improving the efficiency.

  14. Evaluation of urban sprawl and urban landscape pattern in a rapidly developing region.

    Science.gov (United States)

    Lv, Zhi-Qiang; Dai, Fu-Qiang; Sun, Cheng

    2012-10-01

    Urban sprawl is a worldwide phenomenon happening particularly in rapidly developing regions. A study on the spatiotemporal characteristics of urban sprawl and urban pattern is useful for the sustainable management of land management and urban land planning. The present research explores the spatiotemporal dynamics of urban sprawl in the context of a rapid urbanization process in a booming economic region of southern China from 1979 to 2005. Three urban sprawl types are distinguished by analyzing overlaid urban area maps of two adjacent study years which originated from the interpretation of remote sensed images and vector land use maps. Landscape metrics are used to analyze the spatiotemporal pattern of urban sprawl for each study period. Study results show that urban areas have expanded dramatically, and the spatiotemporal landscape pattern configured by the three sprawl types changed obviously. The different sprawl type patterns in five study periods have transformed significantly, with their proportions altered both in terms of quantity and of location. The present research proves that urban sprawl quantification and pattern analysis can provide a clear perspective of the urbanization process during a long time period. Particularly, the present study on urban sprawl and sprawl patterns can be used by land use and urban planners.

  15. Development of a fluorescence-based sensor for rapid diagnosis of cyanide exposure.

    Science.gov (United States)

    Jackson, Randy; Oda, Robert P; Bhandari, Raj K; Mahon, Sari B; Brenner, Matthew; Rockwood, Gary A; Logue, Brian A

    2014-02-04

    Although commonly known as a highly toxic chemical, cyanide is also an essential reagent for many industrial processes in areas such as mining, electroplating, and synthetic fiber production. The "heavy" use of cyanide in these industries, along with its necessary transportation, increases the possibility of human exposure. Because the onset of cyanide toxicity is fast, a rapid, sensitive, and accurate method for the diagnosis of cyanide exposure is necessary. Therefore, a field sensor for the diagnosis of cyanide exposure was developed based on the reaction of naphthalene dialdehyde, taurine, and cyanide, yielding a fluorescent β-isoindole. An integrated cyanide capture "apparatus", consisting of sample and cyanide capture chambers, allowed rapid separation of cyanide from blood samples. Rabbit whole blood was added to the sample chamber, acidified, and the HCN gas evolved was actively transferred through a stainless steel channel to the capture chamber containing a basic solution of naphthalene dialdehyde (NDA) and taurine. The overall analysis time (including the addition of the sample) was cyanide exposure. Most importantly, the sensor was 100% accurate in diagnosing cyanide poisoning for acutely exposed rabbits.

  16. Development of novel hybrid poly(l-lactide)/chitosan scaffolds using the rapid freeze prototyping technique

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, N; Chen, X B [Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Saskatchewan (Canada); Li, M G [Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, Saskatchewan (Canada); Cooper, D, E-mail: xbc719@mail.usask.ca [Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)

    2011-09-15

    Engineered scaffolds have been shown to be critical to various tissue engineering applications. This paper presents the development of a novel three-dimensional scaffold made from a mixture of chitosan microspheres (CMs) and poly(l-lactide) by means of the rapid freeze prototyping (RFP) technique. The CMs were used to encapsulate bovine serum albumin (BSA) and improve the scaffold mechanical properties. Experiments to examine the BSA release were carried out; the BSA release could be controlled by adjusting the crosslink degree of the CMs and prolonged after the CMs were embedded into the PLLA scaffolds, while the examination of the mechanical properties of the scaffolds illustrates that they depend on the ratio of CMs to PLLA in the scaffolds as well as the cryogenic temperature used in the RFP fabrication process. The chemical characteristics of the PLLA/chitosan scaffolds were evaluated by Fourier transform infrared (FTIR) spectroscopy. The morphological and pore structure of the scaffolds were also examined by scanning electron microscopy and micro-tomography. The results obtained show that the scaffolds have higher porosity and enhanced pore size distribution compared to those fabricated by the dispensing-based rapid prototyping technique. This study demonstrates that the novel scaffolds have not only enhanced porous structure and mechanical properties but also showed the potential to preserve the bioactivities of the biomolecules and to control the biomolecule distribution and release rate.

  17. Development of a novel multiplex PCR assay for rapid detection of virulence associated genes of Pasteurella multocida from pigs

    National Research Council Canada - National Science Library

    Rajkhowa, S

    2015-01-01

    Significance and Impact of the Study: The study reports the development and evaluation of a novel multiplex PCR assay for the rapid detection of 11 important VAGs of Pasteurella multocida isolates from pigs...

  18. Anesthetics rapidly promote synaptogenesis during a critical period of brain development.

    Directory of Open Access Journals (Sweden)

    Mathias De Roo

    Full Text Available Experience-driven activity plays an essential role in the development of brain circuitry during critical periods of early postnatal life, a process that depends upon a dynamic balance between excitatory and inhibitory signals. Since general anesthetics are powerful pharmacological modulators of neuronal activity, an important question is whether and how these drugs can affect the development of synaptic networks. To address this issue, we examined here the impact of anesthetics on synapse growth and dynamics. We show that exposure of young rodents to anesthetics that either enhance GABAergic inhibition or block NMDA receptors rapidly induce a significant increase in dendritic spine density in the somatosensory cortex and hippocampus. This effect is developmentally regulated; it is transient but lasts for several days and is also reproduced by selective antagonists of excitatory receptors. Analyses of spine dynamics in hippocampal slice cultures reveals that this effect is mediated through an increased rate of protrusions formation, a better stabilization of newly formed spines, and leads to the formation of functional synapses. Altogether, these findings point to anesthesia as an important modulator of spine dynamics in the developing brain and suggest the existence of a homeostatic process regulating spine formation as a function of neural activity. Importantly, they also raise concern about the potential impact of these drugs on human practice, when applied during critical periods of development in infants.

  19. Asian migration to Australia: food and health consequences.

    Science.gov (United States)

    Wahlqvist, Mark L

    2002-01-01

    Australia's food and health patterns are inextricably and increasingly linked with Asia. Indigenous Australians arrived in the continent via Asia and have linguistic connections with people who settled in south India; there was interaction and food trade between both South-East Asia and China and northern indigenous Australians over thousands of years. After European settlement in 1788, there have been several and increasing (apart from the period of the infamous White Australian Policy following the Colonial period and Independence, with Federation, in 1901) waves of Asian migration, notably during the gold rush (Chinese), the building of the overland Telegraph (Afghans), the Colombo Plan and Asian student education in Australia from the 1950s onwards (South-Eeast Asians), and with refugees (Vietnamese and mainland Chinese), and business (late twentieth century) and progressive family reunion. Each wave has injected additional food cultural elements and caused a measure of health change for migrants and host citizens. Of principal advantage to Australia has been the progressive diversification of the food supply and associated health protection. This has increased food security and sustainability. The process of Australian eating patterns becoming Asianized is evident through market garden development (and the introduction of new foods), fresh food markets and groceries, restaurants and the development of household cooking skills (often taught by student boarders). Most of the diversification has been with grain (rice), legumes (soy), greens, root vegetables, and various 'exotic fruits'. Food acculturation with migration is generally bi-directional. Thus, for Asians in Australia, there has been a decrease in energy expenditure (and a lower plane of energy throughput), an increase in food energy density (through increased fat and sugary drink intakes), and a decrease in certain health protective foods (lentils, soy, greens) and beverages (tea). This sets the stage

  20. Emerging Asian Economics.

    Science.gov (United States)

    Trezise, Philip H.

    What we can expect in the future from the miracle economies of Japan, South Korea, Taiwan, Singapore, and Hong Kong, whether they pose a threat to the older industrial states of Western Europe and North American, and whether China is to be the next emerging Asian economy are discussed. The amazing economic recovery of these East Asian countries…

  1. Diagnosis and treatment of familial cherubism characterized by early onset and rapid development.

    Science.gov (United States)

    Mortellaro, Carmen; Bello, Lucilla; Lucchina, Alberta Greco; Pucci, Angela

    2009-01-01

    Cherubism is a benign maxillary bone dysplasia of childhood, usually showing an autosomically dominant inheritance with variable penetrance and spontaneously resolving after puberty. Only maxillary bones are affected and develop pseudocystic osteolytic lesions. This article presents an early and rapidly evolving familial case of cherubism. The 3-year-old child underwent conservative curettage of lesions, with a conservative approach that allowed a normal permanent dentition in adolescence. Family history revealed that the father had been treated for similar lesions between 14 and 21 years of age, but the late treatment caused edentulism. In conclusion, although cherubism represents a benign and localized maxillary dysplasia, it requires prompt surgical but conservative treatment and careful follow-up to avoid permanent lesions, that is, malocclusion and/or edentulism.

  2. Rapid Development and Use of a Nationwide Training Program for Cholera Management, Haiti, 2010

    Science.gov (United States)

    Lynch, Michael; Lambert, Yves; Sobel, Jeremy; Domerçant, Jean W.; Khan, Azharul

    2011-01-01

    When epidemic cholera appeared in Haiti in October 2010, the medical community there had virtually no experience with the disease and needed rapid training as the epidemic spread throughout the country. We developed a set of training materials specific to Haiti and launched a cascading training effort. Through a training-of-trainers course in November 14–15, 2010, and department-level training conducted in French and Creole over the following 3 weeks, 521 persons were trained and equipped to further train staff at the institutions where they worked. After the training, the hospitalized cholera patients’ case-fatality rate dropped from 4% to <2% by mid-December and was <1% by January 2011. Continuing in-service training, monitoring and evaluation, and integration of cholera management into regular clinical training will help sustain this success. PMID:22099112

  3. Development of Middle Stone Age innovation linked to rapid climate change.

    Science.gov (United States)

    Ziegler, Martin; Simon, Margit H; Hall, Ian R; Barker, Stephen; Stringer, Chris; Zahn, Rainer

    2013-01-01

    The development of modernity in early human populations has been linked to pulsed phases of technological and behavioural innovation within the Middle Stone Age of South Africa. However, the trigger for these intermittent pulses of technological innovation is an enigma. Here we show that, contrary to some previous studies, the occurrence of innovation was tightly linked to abrupt climate change. Major innovational pulses occurred at times when South African climate changed rapidly towards more humid conditions, while northern sub-Saharan Africa experienced widespread droughts, as the Northern Hemisphere entered phases of extreme cooling. These millennial-scale teleconnections resulted from the bipolar seesaw behaviour of the Atlantic Ocean related to changes in the ocean circulation. These conditions led to humid pulses in South Africa and potentially to the creation of favourable environmental conditions. This strongly implies that innovational pulses of early modern human behaviour were climatically influenced and linked to the adoption of refugia.

  4. [Development and scientific rationale for a universal index for rapid assessment of readability].

    Science.gov (United States)

    Teksheva, L M

    2007-01-01

    The hygienic regulations of academic books, including teachware, for children and adolescents necessitate the performance of special studies to assess their readability by the unified procedure that would suggest a visual response while reading. This study has developed and warranted a universal readability index by the velocity of reading. There are significant regression correlations between the velocity of reading as the readability index and the degree of tiredness, detected by various visual functions. The parameters of accommodation and adaptive properties of the eye are most sensitive to visual load on reading. Reading velocity as an indicator of readability of illogical texts may be used as a rapid method under laboratory and natural conditions to determine the action of various textual loads on the visual system.

  5. Development of cross-priming amplification assays for rapid and sensitive detection of Aeromonas hydrophila.

    Science.gov (United States)

    Meng, S; Wang, Y; Wang, Y; Liu, D; Ye, C

    2015-08-01

    Aeromonas hydrophila has been increasingly implicated as the aetiologic agent of various human diseases. Therefore, reliable laboratory detection and identification of this bacterium has become clinically and epidemiologically desirable. We developed a nearly instrument-free, simple molecular method for rapid detection of Aer. hydrophila using a cross-priming amplification (CPA) assay with the desA gene as the target. The desA gene is crucial for the survival and growth of Aer. hydrophila under iron starvation. The results can be visualized as colour changes without opening the reaction tubes. No false-positive results were observed for the 33 non-Aer. hydrophila strains tested to evaluate assay specificity. The limit of detection for Aer. hydrophila was approximately 200 copies of desA per reaction (on reference plasmids) and 5 × 10(3)  CFU g(-1) Aer. hydrophila in simulated human stool, which is the same sensitivity as a qPCR assay. The performance of the CPA assay was also evaluated with 100 stool specimens from diarrhoea patients and 40 environmental water samples. In conclusion, the simplicity, cost-effectiveness and nearly instrument-free platform of the CPA assay make it practical for use in primary care facilities and smaller clinical laboratories. Aeromonas hydrophila is a human pathogen that infects via exposed wounds or ingestion of contaminated water and food. In this study, a CPA-based PCR method was developed for specific, rapid, cost-effective detection of Aer. hydrophila, and the test results could be visualized without opening the reaction tubes. This is the first report on the application of the CPA method for the detection of Aer. hydrophila. This novel method could be practical for use in primary care facilities and smaller clinical laboratories. © 2015 The Society for Applied Microbiology.

  6. Development and testing of a rapid diagnostic test for bubonic and pneumonic plague.

    Science.gov (United States)

    Chanteau, Suzanne; Rahalison, Lila; Ralafiarisoa, Lalao; Foulon, Jeanine; Ratsitorahina, Mahery; Ratsifasoamanana, Lala; Carniel, Elisabeth; Nato, Farida

    2003-01-18

    Plague is often fatal without prompt and appropriate treatment. It affects mainly poor and remote populations. Late diagnosis is one of the major causes of human death and spread of the disease, since it limits the effectiveness of control measures. We aimed to develop and assess a rapid diagnostic test (RDT) for plague. We developed a test that used monoclonal antibodies to the F1 antigen of Yersinia pestis. Sensitivity and specificity were assessed with a range of bacterial cultures and clinical samples, and compared with findings from available ELISA and bacteriological tests for plague. Samples from patients thought to have plague were tested with the RDT in the laboratory and by health workers in 26 pilot sites in Madagascar. The RDT detected concentrations of F1 antigen as low as 0.5 ng/mL in up to 15 min, and had a shelf life of 21 days at 60 degrees C. Its sensitivity and specificity were both 100%. RDT detected 41.6% and 31% more positive clinical specimens than did bacteriological methods and ELISA, respectively. The agreement rate between tests done at remote centres and in the laboratory was 89.8%. With the combination of bacteriological methods and F1 ELISA as reference standard, the positive and negative predictive values of the RDT were 90.6% and 86.7%, respectively. Our RDT is a specific, sensitive, and reliable test that can easily be done by health workers at the patient's bedside, for the rapid diagnosis of pneumonic and bubonic plague. This test will be of key importance for the control of plague in endemic countries.

  7. Using aerial photography to estimate riparian zone impacts in a rapidly developing river corridor.

    Science.gov (United States)

    Owers, Katharine A; Albanese, Brett; Litts, Thomas

    2012-03-01

    Riparian zones are critical for protecting water quality and wildlife, but are often impacted by human activities. Ongoing threats and uncertainty about the effectiveness of buffer regulations emphasize the importance of monitoring riparian buffers through time. We developed a method to rapidly categorize buffer width and landuse attributes using 2007 leaf-on aerial photography and applied it to a 65 km section of the Toccoa River in north Georgia. We repeated our protocol using 1999 leaf-off aerial photographs to assess the utility of our approach for monitoring. Almost half (45%) of the length of the Toccoa River was bordered by buffers less than 50 ft wide in 2007, with agricultural and built-up lands having the smallest buffers. The percentage of river length in each buffer width category changed little between 1999 and 2007, but we did detect a 5% decrease in agricultural land use, a corresponding increase in built-up land use, and an additional 149 buildings within 100 ft of the river. Field verification indicated that our method overestimated buffer widths and forested land use and underestimated built-up land use and the number of buildings within 100 ft of the river. Our methodology can be used to rapidly assess the status of riparian buffers. Including supplemental data (e.g., leaf-off imagery, road layers) will allow detection of the fine-scale impacts underestimated in our study. Our results on the Toccoa River reflect historic impacts, exemptions and variances to regulations, and the ongoing threat of vacation home development. We recommend additional monitoring, improvements in policy, and efforts to increase voluntary protection and restoration of stream buffers.

  8. Rapid-X - An FPGA Development Toolset Using a Custom Simulink Library for MTCA.4 Modules

    Science.gov (United States)

    Prędki, Paweł; Heuer, Michael; Butkowski, Łukasz; Przygoda, Konrad; Schlarb, Holger; Napieralski, Andrzej

    2015-06-01

    The recent introduction of advanced hardware architectures such as the Micro Telecommunications Computing Architecture (MTCA) caused a change in the approach to implementation of control schemes in many fields. The development has been moving away from traditional programming languages ( C/C++), to hardware description languages (VHDL, Verilog), which are used in FPGA development. With MATLAB/Simulink it is possible to describe complex systems with block diagrams and simulate their behavior. Those diagrams are then used by the HDL experts to implement exactly the required functionality in hardware. Both the porting of existing applications and adaptation of new ones require a lot of development time from them. To solve this, Xilinx System Generator, a toolbox for MATLAB/Simulink, allows rapid prototyping of those block diagrams using hardware modelling. It is still up to the firmware developer to merge this structure with the hardware-dependent HDL project. This prevents the application engineer from quickly verifying the proposed schemes in real hardware. The framework described in this article overcomes these challenges, offering a hardware-independent library of components that can be used in Simulink/System Generator models. The components are subsequently translated into VHDL entities and integrated with a pre-prepared VHDL project template. Furthermore, the entire implementation process is run in the background, giving the user an almost one-click path from control scheme modelling and simulation to bit-file generation. This approach allows the application engineers to quickly develop new schemes and test them in real hardware environment. The applications may range from simple data logging or signal generation ones to very advanced controllers. Taking advantage of the Simulink simulation capabilities and user-friendly hardware implementation routines, the framework significantly decreases the development time of FPGA-based applications.

  9. AIBench: a rapid application development framework for translational research in biomedicine.

    Science.gov (United States)

    Glez-Peña, D; Reboiro-Jato, M; Maia, P; Rocha, M; Díaz, F; Fdez-Riverola, F

    2010-05-01

    Applied research in both biomedical discovery and translational medicine today often requires the rapid development of fully featured applications containing both advanced and specific functionalities, for real use in practice. In this context, new tools are demanded that allow for efficient generation, deployment and reutilization of such biomedical applications as well as their associated functionalities. In this context this paper presents AIBench, an open-source Java desktop application framework for scientific software development with the goal of providing support to both fundamental and applied research in the domain of translational biomedicine. AIBench incorporates a powerful plug-in engine, a flexible scripting platform and takes advantage of Java annotations, reflection and various design principles in order to make it easy to use, lightweight and non-intrusive. By following a basic input-processing-output life cycle, it is possible to fully develop multiplatform applications using only three types of concepts: operations, data-types and views. The framework automatically provides functionalities that are present in a typical scientific application including user parameter definition, logging facilities, multi-threading execution, experiment repeatability and user interface workflow management, among others. The proposed framework architecture defines a reusable component model which also allows assembling new applications by the reuse of libraries from past projects or third-party software. Copyright (c) 2009 Elsevier Ireland Ltd. All rights reserved.

  10. Isolation, amplification and characterization of foodborne pathogen disease bacteria gene for rapid kit test development

    Science.gov (United States)

    Nurjayadi, M.; Santoso, I.; Kartika, I. R.; Kurniadewi, F.; Saamia, V.; Sofihan, W.; Nurkhasanah, D.

    2017-07-01

    There is a lot of public concern over food safety. Food-safety cases recently, including many food poisoning cases in both the developed and developing countries, considered to be the national security threats which involved police investigation. Quick and accurate detection methods are needed to handle the food poisoning cases with a big number of sufferers at the same time. Therefore, the research is aimed to develop a specific, sensitive, and rapid result molecular detection tool for foodborne pathogen bacteria. We, thus, propose genomic level approach with Polymerase Chain Reaction. The research has successfully produced a specific primer to perform amplification to fim-C S. typhi, E. coli, and pef Salmonella typhimurium genes. The electrophoresis result shows that amplification products are 95 base pairs, 121 base pairs, and 139 base pairs; and all three genes are in accordance with the size of the in silico to third genes bacteria. In conclusion, the research has been successfully designed a specific detection tool to three foodborne pathogen bacteria genes. Further stages test and the uses of Real-time PCR in the detection are still in the trial process for better detection method.

  11. Evaluation of Proteus as a Tool for the Rapid Development of Models of Hydrologic Systems

    Science.gov (United States)

    Weigand, T. M.; Farthing, M. W.; Kees, C. E.; Miller, C. T.

    2013-12-01

    Models of modern hydrologic systems can be complex and involve a variety of operators with varying character. The goal is to implement approximations of such models that are both efficient for the developer and computationally efficient, which is a set of naturally competing objectives. Proteus is a Python-based toolbox that supports prototyping of model formulations as well as a wide variety of modern numerical methods and parallel computing. We used Proteus to develop numerical approximations for three models: Richards' equation, a brine flow model derived using the Thermodynamically Constrained Averaging Theory (TCAT), and a multiphase TCAT-based tumor growth model. For Richards' equation, we investigated discontinuous Galerkin solutions with higher order time integration based on the backward difference formulas. The TCAT brine flow model was implemented using Proteus and a variety of numerical methods were compared to hand coded solutions. Finally, an existing tumor growth model was implemented in Proteus to introduce more advanced numerics and allow the code to be run in parallel. From these three example models, Proteus was found to be an attractive open-source option for rapidly developing high quality code for solving existing and evolving computational science models.

  12. Late winter feeding stimulates rapid spring development of carniolan honey bee colonies (Apis mellifera carnica

    Directory of Open Access Journals (Sweden)

    Zlatko Puškadija

    2017-01-01

    Full Text Available Unfavourable weather conditions after the queen starts with intensive oviposition during early spring may cause an imbalance in the division of tasks among worker bees in the bee colony. This can lead to slow spring development and poor exploitation of the main spring nectar flows. In order to accelerate the spring development, it is necessary, as a technological measure, to feed supplemental candy to bee colonies. In this research, the necessity of supplemental feeding, as well as the composition of candy (pollen and protein substitute were analysed. Three groups of ten bee colonies each were formed - the control, unfed group, pollen candy fed and protein substitute candy fed. In the period from 22/02/2016 and 04/04/2016 three control measurements were performed during which the number of bees, the number of brood cells and weight of the bee colonies were determined. The research has shown that supplemental feeding of the bee colony in late winter in order to encourage the rapid spring development is justified. Namely, at the final measurements in April, the results showed differences between groups. The treated colonies had higher net hive weight, a greater number of bees and statistically significantly more brood cells. The results of this study confirm that the technological measure of supplemental feeding in late winter should be performed on all commercial apiaries for the production of honey, pollen, royal jelly, queen bees and bee venom.

  13. Overcoming challenges to initiating cell therapy clinical trials in rapidly developing countries: India as a model.

    Science.gov (United States)

    Viswanathan, Sowmya; Rao, Mahendra; Keating, Armand; Srivastava, Alok

    2013-08-01

    Increasingly, a number of rapidly developing countries, including India, China, Brazil, and others, are becoming global hot spots for the development of regenerative medicine applications, including stem cell-based therapies. Identifying and overcoming regulatory and translational research challenges and promoting scientific and ethical clinical trials with cells will help curb the growth of stem cell tourism for unproven therapies. It will also enable academic investigators, local regulators, and national and international biotechnology and biopharmaceutical companies to accelerate stem cell-based clinical research that could lead to effective innovative treatments in these regions. Using India as a model system and obtaining input from regulators, clinicians, academics, and industry representatives across the stem cell field in India, we reviewed the role of key agencies and processes involved in this field. We have identified areas that need attention and here provide solutions from other established and functioning models in the world to streamline and unify the regulatory and ethics approval processes for cell-based therapies. We also make recommendations to check the growth and functioning of clinics offering unproven treatments. Addressing these issues will remove considerable hurdles to both local and international investigators, accelerate the pace of research and development, and create a quality environment for reliable products to emerge. By doing so, these countries would have taken one important step to move to the forefront of stem cell-based therapeutics.

  14. Development of compact rapid charging power supply for capacitive energy storage in pulsed power drivers.

    Science.gov (United States)

    Sharma, Surender Kumar; Shyam, Anurag

    2015-02-01

    High energy capacitor bank is used for primary electrical energy storage in pulsed power drivers. The capacitors used in these pulsed power drivers have low inductance, low internal resistance, and less dc life, so it has to be charged rapidly and immediately discharged into the load. A series resonant converter based 45 kV compact power supply is designed and developed for rapid charging of the capacitor bank with constant charging current up to 150 mA. It is short circuit proof, and zero current switching technique is used to commute the semiconductor switch. A high frequency resonant inverter switching at 10 kHz makes the overall size small and reduces the switching losses. The output current of the power supply is limited by constant on-time and variable frequency switching control technique. The power supply is tested by charging the 45 kV/1.67 μF and 15 kV/356 μF capacitor banks. It has charged the capacitor bank up to rated voltage with maximum charging current of 150 mA and the average charging rate of 3.4 kJ/s. The output current of the power supply is limited by reducing the switching frequency at 5 kHz, 3.3 kHz, and 1.7 kHz and tested with 45 kV/1.67 μF capacitor bank. The protection circuit is included in the power supply for over current, under voltage, and over temperature. The design details and the experimental testing results of the power supply for resonant current, output current, and voltage traces of the power supply with capacitive, resistive, and short circuited load are presented and discussed.

  15. Anemia management: development of a rapid-access anemia and intravenous iron service

    Directory of Open Access Journals (Sweden)

    Radia D

    2013-08-01

    Full Text Available Deepti Radia,1 Ibrahim Momoh,2 Richard Dillon,1 Yvonne Francis,1 Laura Cameron,1 Toni-Lee Fagg,1 Hannah Overland,1 Susan Robinson,1 Claire N Harrison11Haematology Department, Guy's and St Thomas' NHS Foundation Trust, London, UK; 2Bupa Home Healthcare, Harlow, UKAbstract: This article describes the initiation and evolution of the Rapid-Access Anemia Clinic (RAAC at Guy's and St Thomas' Hospitals, London, UK. This clinic was set up to provide diagnosis and treatment, and to coordinate investigative procedures, where necessary, into the underlying causes of anemia. Initially piloted with anemic preoperative orthopedic patients, the clinic now treats a wide range of conditions, deriving from both internal and external referrals. Treatment includes dietary advice, supplementation with iron, vitamin B12 and folate, and blood transfusion. Most patients at the RAAC need iron replacement, the majority of which require intravenous (IV iron. Therefore the first-line IV iron-administration protocol is carefully considered to ensure viability of the service and patient satisfaction. Four IV irons available in the UK are discussed, with explanation of the benefits and drawbacks of each product and the reasoning behind the IV iron choice at different stages of the RAAC's development. Costs to the service, affected by IV iron price and administration regimen, are considered, as well as the product's contraindications. Finally, the authors reflect on the success of the RAAC and how it has improved patients' quality-of-treatment experience, in addition to benefiting the hospital and National Health Service in achieving specific health-care mandates and directives. Drawing from the authors' experiences, recommendations are given to assist others in setting up and providing a successful rapid-access anemia service or similar facility.Keywords: hemoglobin, iron deficiency, ferric carboxymaltose, iron sucrose, iron dextran, iron isomaltoside

  16. Evaluation of nine HIV rapid test kits to develop a national HIV testing algorithm in Nigeria

    Directory of Open Access Journals (Sweden)

    Orji Bassey

    2015-05-01

    Full Text Available Background: Non-cold chain-dependent HIV rapid testing has been adopted in many resource-constrained nations as a strategy for reaching out to populations. HIV rapid test kits (RTKs have the advantage of ease of use, low operational cost and short turnaround times. Before 2005, different RTKs had been used in Nigeria without formal evaluation. Between 2005 and 2007, a study was conducted to formally evaluate a number of RTKs and construct HIV testing algorithms. Objectives: The objectives of this study were to assess and select HIV RTKs and develop national testing algorithms. Method: Nine RTKs were evaluated using 528 well-characterised plasma samples. These comprised 198 HIV-positive specimens (37.5% and 330 HIV-negative specimens (62.5%, collected nationally. Sensitivity and specificity were calculated with 95% confidence intervals for all nine RTKs singly and for serial and parallel combinations of six RTKs; and relative costs were estimated. Results: Six of the nine RTKs met the selection criteria, including minimum sensitivity and specificity (both ≥ 99.0% requirements. There were no significant differences in sensitivities or specificities of RTKs in the serial and parallel algorithms, but the cost of RTKs in parallel algorithms was twice that in serial algorithms. Consequently, three serial algorithms, comprising four test kits (BundiTM, DetermineTM, Stat-Pak® and Uni-GoldTM with 100.0% sensitivity and 99.1% – 100.0% specificity, were recommended and adopted as national interim testing algorithms in 2007. Conclusion: This evaluation provides the first evidence for reliable combinations of RTKs for HIV testing in Nigeria. However, these RTKs need further evaluation in the field (Phase II to re-validate their performance.

  17. Development of Rapid Detection and Genetic Characterization of Salmonella in Poultry Breeder Feeds

    Directory of Open Access Journals (Sweden)

    Steven C. Ricke

    2009-07-01

    Full Text Available Salmonella is a leading cause of foodborne illness in the United States, with poultry and poultry products being a primary source of infection to humans. Poultry may carry some Salmonella serovars without any signs or symptoms of disease and without causing any adverse effects to the health of the bird. Salmonella may be introduced to a flock by multiple environmental sources, but poultry feed is suspected to be a leading source. Detecting Salmonella in feed can be challenging because low levels of the bacteria may not be recovered using traditional culturing techniques. Numerous detection methodologies have been examined over the years for quantifying Salmonella in feeds and many have proven to be effective for Salmonella isolation and detection in a variety of feeds. However, given the potential need for increased detection sensitivity, molecular detection technologies may the best candidate for developing rapid sensitive methods for identifying small numbers of Salmonella in the background of large volumes of feed. Several studies have been done using polymerase chain reaction (PCR assays and commercial kits to detect Salmonella spp. in a wide variety of feed sources. In addition, DNA array technology has recently been utilized to track the dissemination of a specific Salmonella serotype in feed mills. This review will discuss the processing of feeds and potential points in the process that may introduce Salmonella contamination to the feed. Detection methods currently used and the need for advances in these methods also will be discussed. Finally, implementation of rapid detection for optimizing control methods to prevent and remove any Salmonella contamination of feeds will be considered.

  18. Development of Rapid Detection and Genetic Characterization of Salmonella in Poultry Breeder Feeds

    Science.gov (United States)

    Jarquin, Robin; Hanning, Irene; Ahn, Soohyoun; Ricke, Steven C.

    2009-01-01

    Salmonella is a leading cause of foodborne illness in the United States, with poultry and poultry products being a primary source of infection to humans. Poultry may carry some Salmonella serovars without any signs or symptoms of disease and without causing any adverse effects to the health of the bird. Salmonella may be introduced to a flock by multiple environmental sources, but poultry feed is suspected to be a leading source. Detecting Salmonella in feed can be challenging because low levels of the bacteria may not be recovered using traditional culturing techniques. Numerous detection methodologies have been examined over the years for quantifying Salmonella in feeds and many have proven to be effective for Salmonella isolation and detection in a variety of feeds. However, given the potential need for increased detection sensitivity, molecular detection technologies may the best candidate for developing rapid sensitive methods for identifying small numbers of Salmonella in the background of large volumes of feed. Several studies have been done using polymerase chain reaction (PCR) assays and commercial kits to detect Salmonella spp. in a wide variety of feed sources. In addition, DNA array technology has recently been utilized to track the dissemination of a specific Salmonella serotype in feed mills. This review will discuss the processing of feeds and potential points in the process that may introduce Salmonella contamination to the feed. Detection methods currently used and the need for advances in these methods also will be discussed. Finally, implementation of rapid detection for optimizing control methods to prevent and remove any Salmonella contamination of feeds will be considered. PMID:22346699

  19. Development and validation of a rapid, aldehyde dehydrogenase bright-based cord blood potency assay.

    Science.gov (United States)

    Shoulars, Kevin; Noldner, Pamela; Troy, Jesse D; Cheatham, Lynn; Parrish, Amanda; Page, Kristin; Gentry, Tracy; Balber, Andrew E; Kurtzberg, Joanne

    2016-05-12

    Banked, unrelated umbilical cord blood provides access to hematopoietic stem cell transplantation for patients lacking matched bone marrow donors, yet 10% to 15% of patients experience graft failure or delayed engraftment. This may be due, at least in part, to inadequate potency of the selected cord blood unit (CBU). CBU potency is typically assessed before cryopreservation, neglecting changes in potency occurring during freezing and thawing. Colony-forming units (CFUs) have been previously shown to predict CBU potency, defined as the ability to engraft in patients by day 42 posttransplant. However, the CFU assay is difficult to standardize and requires 2 weeks to perform. Consequently, we developed a rapid multiparameter flow cytometric CBU potency assay that enumerates cells expressing high levels of the enzyme aldehyde dehydrogenase (ALDH bright [ALDH(br)]), along with viable CD45(+) or CD34(+) cell content. These measurements are made on a segment that was attached to a cryopreserved CBU. We validated the assay with prespecified criteria testing accuracy, specificity, repeatability, intermediate precision, and linearity. We then prospectively examined the correlations among ALDH(br), CD34(+), and CFU content of 3908 segments over a 5-year period. ALDH(br) (r = 0.78; 95% confidence interval [CI], 0.76-0.79), but not CD34(+) (r = 0.25; 95% CI, 0.22-0.28), was strongly correlated with CFU content as well as ALDH(br) content of the CBU. These results suggest that the ALDH(br) segment assay (based on unit characteristics measured before release) is a reliable assessment of potency that allows rapid selection and release of CBUs from the cord blood bank to the transplant center for transplantation. © 2016 by The American Society of Hematology.

  20. Development and validation of a rapid, aldehyde dehydrogenase bright–based cord blood potency assay

    Science.gov (United States)

    Noldner, Pamela; Troy, Jesse D.; Cheatham, Lynn; Parrish, Amanda; Page, Kristin; Gentry, Tracy; Balber, Andrew E.; Kurtzberg, Joanne

    2016-01-01

    Banked, unrelated umbilical cord blood provides access to hematopoietic stem cell transplantation for patients lacking matched bone marrow donors, yet 10% to 15% of patients experience graft failure or delayed engraftment. This may be due, at least in part, to inadequate potency of the selected cord blood unit (CBU). CBU potency is typically assessed before cryopreservation, neglecting changes in potency occurring during freezing and thawing. Colony-forming units (CFUs) have been previously shown to predict CBU potency, defined as the ability to engraft in patients by day 42 posttransplant. However, the CFU assay is difficult to standardize and requires 2 weeks to perform. Consequently, we developed a rapid multiparameter flow cytometric CBU potency assay that enumerates cells expressing high levels of the enzyme aldehyde dehydrogenase (ALDH bright [ALDHbr]), along with viable CD45+ or CD34+ cell content. These measurements are made on a segment that was attached to a cryopreserved CBU. We validated the assay with prespecified criteria testing accuracy, specificity, repeatability, intermediate precision, and linearity. We then prospectively examined the correlations among ALDHbr, CD34+, and CFU content of 3908 segments over a 5-year period. ALDHbr (r = 0.78; 95% confidence interval [CI], 0.76-0.79), but not CD34+ (r = 0.25; 95% CI, 0.22-0.28), was strongly correlated with CFU content as well as ALDHbr content of the CBU. These results suggest that the ALDHbr segment assay (based on unit characteristics measured before release) is a reliable assessment of potency that allows rapid selection and release of CBUs from the cord blood bank to the transplant center for transplantation. PMID:26968535

  1. Asian dust exposure triggers acute myocardial infarction.

    Science.gov (United States)

    Kojima, Sunao; Michikawa, Takehiro; Ueda, Kayo; Sakamoto, Tetsuo; Matsui, Kunihiko; Kojima, Tomoko; Tsujita, Kenichi; Ogawa, Hisao; Nitta, Hiroshi; Takami, Akinori

    2017-08-29

    To elucidate whether Asian dust is associated with the incidence of acute myocardial infarction (AMI) and to clarify whether patients who are highly sensitive to Asian dust will develop AMI. Twenty-one participating institutions located throughout Kumamoto Prefecture and capable of performing coronary intervention were included in the study. Data for ground-level observations of Asian dust events were measured at the Kumamoto Local Meteorological Observatory. Data collected between 1 April 2010 and 31 March 2015 were analysed, and 3713 consecutive AMI patients were included. A time-stratified case-crossover design was applied to examine the association between Asian dust exposure and AMI. The occurrence of Asian dust events at 1 day before the onset of AMI was associated with the incidence of AMI [odds ratio (OR), 1.46; 95% confidence interval (CI), 1.09-1.95] and especially, non-ST-segment elevation myocardial infarction was significant (OR 2.03; 95% CI, 1.30-3.15). A significant association between AMI and Asian dust was observed in patients with age ≥75 years, male sex, hypertension, diabetes mellitus, never-smoking status, and chronic kidney disease (CKD). However, Asian dust events had a great impact on AMI onset in patients with CKD (P Asian dust events was found to be significantly associated with AMI incidence among patients with a risk score of 5-6 (OR 2.45; 95% CI: 1.14-5.27). Asian dust events may lead to AMI and have a great impact on its onset in patients with CKD.

  2. Partnership: A Development Strategy for Children. Learning from Redd Barna's Experience in Asia. Asian Dialogue No. 5.

    Science.gov (United States)

    Wazir, Rekha; van Oudenhoven, Nico; Ageros, Bengt, Ed.

    This report explores the partnerships between local development organizations and Redd Barna (RB), a Norwegian development organization engaged in child-centered development work in Cambodia, Nepal, Sri Lanka, and Thailand. Chapter 1 of the report introduces the concept of partnership as viewed from donor and recipient perspectives, describes the…

  3. Life history, reproductive biology, and larval development of Ontsira mellipes (Hymenoptera: Braconidae), a newly associated parasitoid of the invasive Asian longhorned beetle (Coleoptera: Cerambycidae

    Science.gov (United States)

    The Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), is an invasive xylophagous beetle originating from Asia. Several endemic North American hymenopteran (Braconidae) species located in the mid-Atlantic region were found attacking and reproducing on A. glabripennis larvae. Ontsira ...

  4. Food Safety Program in Asian Countries.

    Science.gov (United States)

    Yamaguchi, Ryuji; Hwang, Lucy Sun

    2015-01-01

    By using the ILSI network in Asia, we are holding a session focused on food safety programs in several Asian areas. In view of the external environment, it is expected to impact the global food system in the near future, including the rapid increase in food demand and in public health services due to population growth, as well as the threats to biosecurity and food safety due to the rapid globalization of the food trade. Facilitating effective information sharing holds promise for the activation of the food industry. At this session, Prof. Hwang shares the current situation of Food Safety and Sanitation Regulations in Taiwan. Dr. Liu provides a talk on the role of risk assessment in food regulatory control focused on aluminum-containing food additives in China. After the JECFA evaluation of aluminum-containing food additives in 2011, each country has carried out risk assessment based on dietary intake surveys. Ms. Chan reports on the activities of a working group on Food Standards Harmonization in ASEAN. She also explains that the ILSI Southeast Asia Region has actively supported the various ASEAN Working Groups in utilizing science to harmonize food standards. Prof. Park provides current research activities in Korea focused on the effect of climate change on food safety. Climate change is generally seen as having a negative impact on food security, particularly in developing countries. We use these four presentations as a springboard to vigorous discussion on issues related to Food Safety in Asia.

  5. Development of materials for the rapid manufacture of die cast tooling

    Science.gov (United States)

    Hardro, Peter Jason

    The focus of this research is to develop a material composition that can be processed by rapid prototyping (RP) in order to produce tooling for the die casting process. Where these rapidly produced tools will be superior to traditional tooling production methods by offering one or more of the following advantages: reduced tooling cost, shortened tooling creation time, reduced man-hours for tool creation, increased tool life, and shortened die casting cycle time. By utilizing RP's additive build process and vast material selection, there was a prospect that die cast tooling may be produced quicker and with superior material properties. To this end, the material properties that influence die life and cycle time were determined, and a list of materials that fulfill these "optimal" properties were highlighted. Physical testing was conducted in order to grade the processability of each of the material systems and to optimize the manufacturing process for the downselected material system. Sample specimens were produced and microscopy techniques were utilized to determine a number of physical properties of the material system. Additionally, a benchmark geometry was selected and die casting dies were produced from traditional tool materials (H13 steel) and techniques (machining) and from the newly developed materials and RP techniques (selective laser sintering (SLS) and laser engineered net shaping (LENS)). Once the tools were created, a die cast alloy was selected and a preset number of parts were shot into each tool. During tool creation, the manufacturing time and cost was closely monitored and an economic model was developed to compare traditional tooling to RP tooling. This model allows one to determine, in the early design stages, when it is advantageous to implement RP tooling and when traditional tooling would be best. The results of the physical testing and economic analysis has shown that RP tooling is able to achieve a number of the research objectives, namely

  6. Development of loop-mediated isothermal amplification for rapid detection of Entamoeba histolytica.

    Science.gov (United States)

    Rivera, Windell L; Ong, Vanissa A

    2013-06-01

    To develop a loop-mediated isothermal amplification (LAMP) assay for the detection of Entamoeba histolytica E. histolytica, the causative agent of amebiasis. The LAMP primer set was designed from E. histolytica hemolysin gene HLY6. Genomic DNA of E. histolytica trophozoites strain HK9 was used to optimize the LAMP mixture and conditions. Amplification of DNA in the LAMP mixture was monitored through visual inspection for turbidity of the LAMP mix as well as addition of fluorescent dye. Positive LAMP reactions turned turbid while negative ones remained clear. Upon addition of a fluorescent dye, all positive reactions turned green while the negative control remained orange under ambient light. After electrophoresis in 1.5% agarose gels, a ladder of multiple bands of different sizes can be observed in positive samples while no bands were detected in the negative control. The sensitivity of the assay was found to be 5 parasites per reaction which corresponds to approximately 15.8 ng/μ L DNA. The specificity of the assay was verified by the absence of amplified products when DNA from other gastrointestinal parasites such as the morphologically similar but non-pathogenic species, Entamoeba dispar 39, and other diarrhea-causing organisms such as Blastocystis hominis and Escherichia coli were used. The LAMP assay we have developed enables the detection of E. histolytica with rapidity and ease, therefore rendering it is suitable for laboratory and field diagnosis of amebiasis. Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  7. SCOUT: developing a modular multimission spacecraft architecture for high-capability rapid access to space

    Science.gov (United States)

    Rogers, Aaron; Shoemaker, James; Jordan, Luis; McDermott, Scott

    2004-08-01

    The long lead and cycle times currently associated with development and launch of satellite systems have established a prohibitive environment for responsive deployment of tactical capability to orbit. With the advent of the RASCAL program - poised to offer launch capability to Low Earth Orbit (LEO) within 24 hours - there is a clear motivation for a comparable, multi-mission, rapidly configurable microsatellite. The SCOUT program is developing the key enabling technologies that will enable this capability while also addressing the production and logistic challenges essential to its implementation. Intrinsic to the design will be a "Plug-and-Sense" capability, which will enable a vehicle to detect the presence and orientation of integrated subsystem modules, as well as ascertain their function, and communicate key performance parameters. The system will utilize a heuristic, self-interrogation approach to provide a robust means of performing configuration and diagnostics activities that transcend nominal housekeeping routines to include an enhanced degree of system autonomy. A minimally structured design, emphasizing a lightweight, interchangeable framework will enable quick integration and deployment, while preserving high on-orbit payload mass fraction. Similarly, the system will also feature a novel approach to assembly, integration, and test activities that spans ground through on-orbit operations.

  8. Ideas for the rapid development of the structural models in mechanical engineering

    Science.gov (United States)

    Oanta, E.; Raicu, A.; Panait, C.

    2017-08-01

    Conceiving computer based instruments is a long run concern of the authors. Some of the original solutions are: optimal processing of the large matrices, interfaces between the programming languages, approximation theory using spline functions, numerical programming increased accuracy based on the extended arbitrary precision libraries. For the rapid development of the models we identified the following directions: atomization, ‘librarization’, parameterization, automatization and integration. Each of these directions has some particular aspects if we approach mechanical design problems or software development. Atomization means a thorough top-down decomposition analysis which offers an insight regarding the basic features of the phenomenon. Creation of libraries of reusable mechanical parts and libraries of programs (data types, functions) save time, cost and effort when a new model must be conceived. Parameterization leads to flexible definition of the mechanical parts, the values of the parameters being changed either using a dimensioning program or in accord to other parts belonging to the same assembly. The resulting templates may be also included in libraries. Original software applications are useful for the model’s input data generation, to input the data into CAD/FEA commercial applications and for the data integration of the various types of studies included in the same project.

  9. Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria.

    Science.gov (United States)

    Markakpo, Uri S; Bosompem, Kwabena M; Dzodzomenyo, Mawuli; Danso-Appiah, Anthony; Essuman, Edward E; Anyan, William K; Suzuki, Mitsuko; Stephens, Judith K; Anim-Baidoo, Isaac; Asmah, Richard H; Ofori, Michael F; Madjitey, Parnor; Danquah, Jonas B; Frempong, Naa Adjeley; Kwofie, Kofi D; Amoa-Bosompem, Michael; Sullivan, David; Fobil, Julius N; Quakyi, Isabella A

    2016-10-01

    To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia. © 2016 John Wiley & Sons Ltd.

  10. Toxicity and sublethal effects of chlorantraniliprole on the development and fecundity of a non-specific predator, the multicolored Asian lady beetle, Harmonia axyridis (Pallas).

    Science.gov (United States)

    Nawaz, Muhammad; Cai, Wanlun; Jing, Zhao; Zhou, Xingmiao; Mabubu, Juma Ibrahim; Hua, Hongxia

    2017-07-01

    In order to further develop integrated pest management (IPM) approaches for controlling insect pests, it is important to estimate the effects of pesticides. In this study, the toxicity and sublethal effects of the insecticide chlorantraniliprole on a non-specific predator, the multicolored Asian lady beetle Harmonia axyridis, were evaluated and life table parameter data were analyzed statistically using the age-stage, two-sex life table procedure. The results of this study show that the development time of second and fourth instar larvae as well as pupa was significantly prolonged in populations treated with LC10 (2.42 mg (a.i.) L -1 ) and LC30 (12.06 mg (a.i.) L -1 ), while adult longevity and fecundity were both significantly reduced and the preoviposition period (POP) was significantly prolonged following treatment compared to the control. In addition, the net reproductive rate (R 0 ), as well as the intrinsic (r) and finite rate of increase (λ) were significantly decreased in groups treated with the insecticide. These results reveal that because sublethal concentrations of chlorantraniliprole impair the population growth of H. axyridis, more attention should be paid to the use of this chemical as a component of IPM strategies. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. SURVEY DESIGN TO GRASP AND COMPARE USER'S ATTITUDES ON BUS RAPID TRANSIT (BRT IN DEVELOPING COUNTRIES

    Directory of Open Access Journals (Sweden)

    Thillaiampalam SIVAKUMAR

    2006-01-01

    Full Text Available In mitigating urban transportation problems and providing a sustainable transit system, rail-based systems have become popular. While rail-based systems are welcome in many developed countries, it is impractical for cities in developing countries due to the high cost of system building and operation. Thus, a staged or incremental adjustment towards fixed guide way transit implementation of greater interest to many agencies today, and these days it has started developing in terms of Bus Rapid Transit (BRT. As it is very new to developing cities, it is a challenge to get the users to understand the system and to grasp their attitude. Besides, there are many other general issues like literacy, lack of a sample frame etc. Survey design needs to be tuned carefully for these cities to obtain a resonant output. Concerning all these problems, a hypothetical questionnaire survey such as Stated Preference (SP has become popular. This study conducted a survey on BRT implementation with SP as a hypothetical tool at a selected corridor in Colombo, the capital of Sri Lanka. The main objectives were to identify questionnaire design(media effects, literacy of users, segmental variation, and the important variable(s. In this survey design, system explanation has been set in two slightly different ways (media: TEXT ∼ IMAGE for comparison and it was found that even a slight difference on design affected the users' response considerably. Income level could not be predicted directly, but car ownership was found to be a good predictor, it was found to be an important variable and it showed a correlation with literacy.

  12. The 2000 activities and the 2nd Workshop on Human Resources Development in the Nuclear Field as part of Asian regional cooperation

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-06-01

    In 1999, the Project for Human Resources Development (HRD) was initiated as defined in the framework of the Forum for Nuclear Cooperation in Asia (FNCA), organized by the Atomic Energy Commission of Japan. The objective of the HRD Project is to solidify the foundation of technologies for nuclear development and utilization in Asia by promoting human resources development in Asian countries. In the Project there are two kind of activities; in-workshop activities and outside-of-workshop activities. As in-workshop activities, the 2nd Workshop on Human Resources Development in the Nuclear Field was held on November 27 and 28, 2000, at the Tokai Research Institute of JAERI. As outside-of-workshop activities. 'The presentation of the present state of international training and education in the nuclear field in Japan' was held on November 29, 2000 after the workshop. Participating countries were China, Indonesia, South Korea, Japan, Malaysia, the Philippines, Thailand, and Vietnam. The secretariat for the Human Resources Development Projects is provided by the Nuclear Technology and Education Center of the Japan Atomic Energy Research Institute. This report consists of presentation papers and materials at the Workshop, presentation documents of 'The present state of international training and education in the nuclear field in Japan', a letter of proposal from the Project Leader of Japan to the project leaders of the participating countries after the Workshop and a presentation paper on Human Resources Development at the 3rd Coordinators Meeting of FNCA at Tokyo on March 14-16, 2001. (author)

  13. The 1999 activities and the 1st seminar on human resources development in the nuclear field as part of Asian regional cooperation (contract research)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-12-01

    In August, 1999, the Project for Human Resources Development (HRD) was initiated as defined in the framework of the Forum for Nuclear Cooperation in Asia, organized by the Atomic Energy Commission based on a resolution of the 10th International Conference for Nuclear Cooperation in Asia, held in March, 1999. The resolution was adopted as a recognition that 'human resources development' was an important area that should be added to the existing fields of cooperation. The Project was organized by the Atomic Energy Bureau of the Science and Technology Agency (STA) and is administrated by the Nuclear Technology and Education Center (NuTEC) of the Japan Atomic Energy Research Institute. The objective of the HRD Project is to solidify the foundation of technologies for nuclear development and utilization in Asia by promoting human resources development in Asian countries. In the Project there are two kind of activities: in-workshop activities and outside-of-workshop activities, as the time of the workshops themselves is too short to achieve the objectives. As In-workshop activities, 1st Seminar on Human Resources Development in the Nuclear Field was held on November 25 and 26, 1999, at the Tokyo International Forum. Participating countries were China, Indonesia, South Korea, Japan, Malaysia, the Philippines, Thailand, and Vietnam, with Australia submitting a study report only. This report consists of presentation papers at the Seminar as in-workshop activities, and a letter of proposal from the project leader of Japan to the project leaders of participating countries after the Seminar and a presentation paper on Human Resources Development at the First Coordinators Meeting on March 7 and 8, 2000 as outside-of-workshop activities. The 10 of the presented papers are indexed individually. (J.P.N.)

  14. Population Dynamics and Educational Development: A Selection of Papers Presented at the Regional Seminar of Experts on Population Dynamics and Educational Planning (Bangkok, Thailand, September 10-18, 1973).

    Science.gov (United States)

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    A selection of papers on Asian population trends and educational development is presented in four parts. Part I defines the major components of Asian population growth as the rapid decline in mortality after 1945, relative increases in the population of less developed regions, accelerated fertility potential, and unequal distribution of wealth.…

  15. The development of a prototype of a “rapid test” for detection of equine antibodies against tetanus

    OpenAIRE

    Stelzmann, Mareike

    2011-01-01

    the following dissertation, the prototype of a “rapid test” for detection of equine antibodies against tetanus is developed. This test makes it possible to detect antibodies against tetanus in equine serum within one hour. Moreover the prototype allows making a statement about the antibody titer. The standard values of the OIE (WORLD ORGANISATION FOR ANIMAL HEALTH, 2009) to validate in vitro-diagnostical tests for veterinary medicine were followed. The rapid test achieves results comparabl...

  16. The Asian Future of Evolutionary Psychology

    Directory of Open Access Journals (Sweden)

    Geoffrey Miller

    2006-01-01

    Full Text Available Asia's population, wealth, cognitive capital, and scientific influence are growing quickly. Reasonable demographic, economic, and psychometric projections suggest that by the mid-21st century, most of the world's psychology will be done in Asia, by Asians. Even if evolutionary psychology wins the battles for academic respectability in the United States and European Union, if it ignores the rise of Asian psychology, it will fail to have any serious, long-term, global influence in the behavioral sciences after the current generations of researchers are dead. I outline a ‘marketing strategy’ for promoting evolutionary psychology in the current Asian powers (Japan, South Korea, Taiwan, Hong Kong, Singapore, the new Asian mega-powers (China, India, and other developing Asia countries (e.g. Vietnam, Thailand, Malaysia, in a way that takes advantage of Asia's relative secularism, freedom from political correctness, sex-positive social attitudes, and intellectual traditions of Hinduism, Buddhism, and Confucianism.

  17. Development of a fluorescence in situ hybridization (FISH) method for rapid detection of Ulva prolifera

    Science.gov (United States)

    Zhang, Qing-Chun; Liu, Qing; Kang, Zhen-Jun; Yu, Ren-Cheng; Yan, Tian; Zhou, Ming-Jiang

    2015-09-01

    Large-scale green tides have occurred consecutively since 2007 in the Yellow Sea (YS), China. The dominant causative species of the green tides has been identified as Ulva prolifera. The origin of green tides in the YS has been traced back to the Subei Shoal based on the results of remote-sensing, numerical simulations and field investigations. However, it is difficult to study the early development of green tides in the Subei Shoal because of the mixture of multiple green algae and the morphological diversity of U. prolifera when under variable environmental conditions. In this study, a rapid and accurate fluorescence in situ hybridization (FISH) method was developed to detect U. prolifera from the community of green algae targeting the 5S rDNA spacer region of U. prolifera. Two specific probes, 5S-1 and 5S-2, were designed based on the sequences of the 5S rDNA spacer regions of U. prolifera, Ulva linza and Ulva flexuosa. Specificity of the FISH method was tested using the six species of green algae commonly occurring in the Subei Shoal, including U. prolifera, U. linza, U. flexuosa, Ulva compressa, Ulva pertusa and Blidingia sp. The results showed that only U. prolifera could be labeled with both probes. Probe 5S-1, which showed a much higher labeling efficiency on U. prolifera, was ultimately selected as the probe for the FISH detection. The sample preparation method was optimized, particularly for the mature green algae, by the addition of cellulase and proteinase K in the pre-hybridization solution. Labeling efficiency with the probe 5S-1 reached 96% on average under the optimized conditions. The successful development of the FISH method has been applied to qualitative and quantitative analysis of field samples collected from the YS, and the results indicate a potential use in future green algae studies.

  18. Development of a prototype lateral flow immunoassay (LFI for the rapid diagnosis of melioidosis.

    Directory of Open Access Journals (Sweden)

    Raymond L Houghton

    2014-03-01

    Full Text Available Burkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis. Isolation of B. pseudomallei from clinical samples is the "gold standard" for the diagnosis of melioidosis; results can take 3-7 days to produce. Alternatively, antibody-based tests have low specificity due to a high percentage of seropositive individuals in endemic areas. There is a clear need to develop a rapid point-of-care antigen detection assay for the diagnosis of melioidosis. Previously, we employed In vivo Microbial Antigen Discovery (InMAD to identify potential B. pseudomallei diagnostic biomarkers. The B. pseudomallei capsular polysaccharide (CPS and numerous protein antigens were identified as potential candidates. Here, we describe the development of a diagnostic immunoassay based on the detection of CPS. Following production of a CPS-specific monoclonal antibody (mAb, an antigen-capture immunoassay was developed to determine the concentration of CPS within a panel of melioidosis patient serum and urine samples. The same mAb was used to produce a prototype Active Melioidosis Detect Lateral Flow Immunoassay (AMD LFI; the limit of detection of the LFI for CPS is comparable to the antigen-capture immunoassay (∼0.2 ng/ml. The analytical reactivity (inclusivity of the AMD LFI was 98.7% (76/77 when tested against a large panel of B. pseudomallei isolates. Analytical specificity (cross-reactivity testing determined that 97.2% of B. pseudomallei near neighbor species (35/36 were not reactive. The non-reactive B. pseudomallei strain and the reactive near neighbor strain can be explained through genetic sequence analysis. Importantly, we show the AMD LFI is capable of detecting CPS in a variety of patient samples. The LFI is currently being evaluated in Thailand and Australia; the focus is to optimize and validate testing procedures on melioidosis patient samples prior to initiation of a large, multisite pre-clinical evaluation.

  19. VaxCelerate II: rapid development of a self-assembling vaccine for Lassa fever.

    Science.gov (United States)

    Leblanc, Pierre; Moise, Leonard; Luza, Cybelle; Chantaralawan, Kanawat; Lezeau, Lynchy; Yuan, Jianping; Field, Mary; Richer, Daniel; Boyle, Christine; Martin, William D; Fishman, Jordan B; Berg, Eric A; Baker, David; Zeigler, Brandon; Mais, Dale E; Taylor, William; Coleman, Russell; Warren, H Shaw; Gelfand, Jeffrey A; De Groot, Anne S; Brauns, Timothy; Poznansky, Mark C

    2014-01-01

    Development of effective vaccines against emerging infectious diseases (EID) can take as much or more than a decade to progress from pathogen isolation/identification to clinical approval. As a result, conventional approaches fail to produce field-ready vaccines before the EID has spread extensively. Lassa is a prototypical emerging infectious disease endemic to West Africa for which no successful vaccine is available. We established the VaxCelerate Consortium to address the need for more rapid vaccine development by creating a platform capable of generating and pre-clinically testing a new vaccine against specific pathogen targets in less than 120 d A self-assembling vaccine is at the core of the approach. It consists of a fusion protein composed of the immunostimulatory Mycobacterium tuberculosis heat shock protein 70 (MtbHSP70) and the biotin binding protein, avidin. Mixing the resulting protein (MAV) with biotinylated pathogen-specific immunogenic peptides yields a self-assembled vaccine (SAV). To meet the time constraint imposed on this project, we used a distributed R&D model involving experts in the fields of protein engineering and production, bioinformatics, peptide synthesis/design and GMP/GLP manufacturing and testing standards. SAV immunogenicity was first tested using H1N1 influenza specific peptides and the entire VaxCelerate process was then tested in a mock live-fire exercise targeting Lassa fever virus. We demonstrated that the Lassa fever vaccine induced significantly increased class II peptide specific interferon-γ CD4(+) T cell responses in HLA-DR3 transgenic mice compared to peptide or MAV alone controls. We thereby demonstrated that our SAV in combination with a distributed development model may facilitate accelerated regulatory review by using an identical design for each vaccine and by applying safety and efficacy assessment tools that are more relevant to human vaccine responses than current animal models.

  20. The positive mental health instrument: development and validation of a culturally relevant scale in a multi-ethnic Asian population

    National Research Council Canada - National Science Library

    Vaingankar, Janhavi Ajit; Subramaniam, Mythily; Chong, Siow Ann; Abdin, Edimansyah; Orlando Edelen, Maria; Picco, Louisa; Lim, Yee Wei; Phua, Mei Yen; Chua, Boon Yiang; Tee, Joseph Y S; Sherbourne, Cathy

    2011-01-01

    .... The objective of this study was to develop a self-administered measure that covers all key and culturally appropriate domains of mental health, which can be applied to compare levels of mental health...

  1. Long-run Overseas Portfolio Diversification Benefits and Opportunities of Asian Emerging Stock Markets and Developed Markets

    National Research Council Canada - National Science Library

    Kasilingam Lingaraja; Murugesan Selvam; Vinayagamoorthi Vasanth; Ramachandran Rajesh Ramkumar

    2015-01-01

    .... This research study investigated the existence of long run portfolio diversification benefits and opportunities of eight emerging stock markets in Asia and three top, benchmark index of Developed Markets...

  2. A tool for rapid assessment of product usability and universal design: development and preliminary psychometric testing.

    Science.gov (United States)

    Lenker, James A; Nasarwanji, Mahiyar; Paquet, Victor; Feathers, David

    2011-01-01

    While there are many available tools and methods to evaluate product usability, few have been tested on user groups with disabilities and even fewer systematically consider universal design principles. This paper describes the development and preliminary psychometric testing of the Rapid Assessment of Product Usability & Universal Design (RAPUUD), a 12-item user-report tool based on the seven principles of universal design. A preliminary set of items was created to elicit ratings of diverse product characteristics (e.g., physical effort, cognitive effort, assistance required, safety). Data were gathered from 61 participants who rated the usability of products they use in their own environments. Each item elicited a full range of responses, with no apparent floor or ceiling effects. Collectively, the 12 items achieved a high internal consistency (Cronbach's α=0.80). The data indicate that the tool was sensitive to differences in functional abilities, as well as differences in product characteristics. The instrument was usable for a range of consumer products, though not all items were appropriate for each and every product. The results suggest that the instrument could become a pragmatic tool for designers to identify usability problems experienced by a diversity of user populations. © 2011 - IOS Press and the authors. All rights reserved

  3. Payments for carbon sequestration to alleviate development pressure in a rapidly urbanizing region

    Science.gov (United States)

    Smith, Jordan W.; Dorning, Monica; Shoemaker, Douglas A.; Méley, Andréanne; Dupey, Lauren; Meentemeyer, Ross K.

    2017-01-01

    The purpose of this study was to determine individuals' willingness to enroll in voluntary payments for carbon sequestration programs through the use of a discrete choice experiment delivered to forest owners living in the rapidly urbanizing region surrounding Charlotte, North Carolina. We examined forest owners' willingness to enroll in payments for carbon sequestration policies under different levels of financial incentives (annual revenue), different contract lengths, and different program administrators (e.g., private companies versus a state or federal agency). We also examined the influence forest owners' sense of place had on their willingness to enroll in hypothetical programs. Our results showed a high level of ambivalence toward participating in payments for carbon sequestration programs. However, both financial incentives and contract lengths significantly influenced forest owners' intent to enroll. Neither program administration nor forest owners' sense of place influenced intent to enroll. Although our analyses indicated that payments from carbon sequestration programs are not currently competitive with the monetary returns expected from timber harvest or property sales, certain forest owners might see payments for carbon sequestration programs as a viable option for offsetting increasing tax costs as development encroaches and property values rise.

  4. The multimod application framework: a rapid application development tool for computer aided medicine.

    Science.gov (United States)

    Viceconti, Marco; Zannoni, Cinzia; Testi, Debora; Petrone, Marco; Perticoni, Stefano; Quadrani, Paolo; Taddei, Fulvia; Imboden, Silvano; Clapworthy, Gordon

    2007-02-01

    This paper describes a new application framework (OpenMAF) for rapid development of multimodal applications in computer-aided medicine. MAF applications are multimodal in data, in representation, and in interaction. The framework supports almost any type of biomedical data, including DICOM datasets, motion-capture recordings, or data from computer simulations (e.g. finite element modeling). The interactive visualization approach (multimodal display) helps the user interpret complex datasets, providing multiple representations of the same data. In addition, the framework allows multimodal interaction by supporting the simultaneous use of different input-output devices like 3D trackers, stereoscopic displays, haptics hardware and speech recognition/synthesis systems. The Framework has been designed to run smoothly even on limited power computers, but it can take advantage of all hardware capabilities. The Framework is based on a collection of portable libraries and it can be compiled on any platform that supports OpenGL, including Windows, MacOS X and any flavor of Unix/linux.

  5. Rapid development of non-alcoholic steatohepatitis in Psammomys obesus (Israeli sand rat.

    Directory of Open Access Journals (Sweden)

    Briana Spolding

    Full Text Available BACKGROUND AND AIMS: A major impediment to establishing new treatments for non-alcoholic steatohepatitis is the lack of suitable animal models that accurately mimic the biochemical and metabolic characteristics of the disease. The aim of this study was to explore a unique polygenic animal model of metabolic disease as a model of non-alcoholic steatohepatitis by determining the effects of 2% dietary cholesterol supplementation on metabolic and liver endpoints in Psammomys obesus (Israeli sand rat. METHODS: P. obesus were provided ad libitum access to either a standard rodent diet (20% kcal/fat or a standard rodent diet supplemented with 2% cholesterol (w/w for 4 weeks. Histological sections of liver from animals on both diets were examined for key features of non-alcoholic steatohepatitis. The expression levels of key genes involved in hepatic lipid metabolism were measured by real-time PCR. RESULTS: P. obesus fed a cholesterol-supplemented diet exhibited profound hepatomegaly and steatosis, and higher plasma transaminase levels. Histological analysis identified extensive steatosis, inflammation, hepatocyte injury and fibrosis. Hepatic gene expression profiling revealed decreased expression of genes involved in delivery and uptake of lipids, and fatty acid and triglyceride synthesis, and increased expression of genes involved in very low density lipoprotein cholesterol synthesis, triglyceride and cholesterol export. CONCLUSIONS: P. obesus rapidly develop non-alcoholic steatohepatitis when fed a cholesterol-supplemented diet that appears to be histologically and mechanistically similar to patients.

  6. Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

    Directory of Open Access Journals (Sweden)

    Yang Yang

    2017-01-01

    Full Text Available Porcine circovirus virus type II (PCV2 is the etiology of postweaning multisystemic wasting syndrome (PMWS, porcine dermatitis, nephropathy syndrome (PDNS, and necrotizing pneumonia. Rapid diagnosis tool for detection of PCV2 plays an important role in the disease control and eradication program. Recombinase polymerase amplification (RPA assays using a real-time fluorescent detection (PCV2 real-time RPA assay and RPA combined with lateral flow dipstick (PCV2 RPA LFD assay were developed targeting the PCV2 ORF2 gene. The results showed that the sensitivity of the PCV2 real-time RPA assay was 102 copies per reaction within 20 min at 37°C and the PCV2 RPA LFD assay had a detection limit of 102 copies per reaction in less than 20 min at 37°C. Both assays were highly specific for PCV2, with no cross-reactions with porcine circovirus virus type 1, foot-and-mouth disease virus, pseudorabies virus, porcine parvovirus, porcine reproductive and respiratory syndrome virus, and classical swine fever virus. Therefore, the RPA assays provide a novel alternative for simple, sensitive, and specific identification of PCV2.

  7. Rapid development of pulmonary cavitation as manifestation of a candida species

    Energy Technology Data Exchange (ETDEWEB)

    Rix, E.; Bickel, R.H.; Baldauf, G.

    1987-01-01

    The roentgenologic pattern of the pulmonary manifestation of candida species, resulting in a rapid development of pulmonary cavitations with mycetoma-like structures, was described in three patients. All patients, undergoing antineoplastic chemotherapy because of acute leukemia, presented with fever and expectoration, which were resistant to various antibiotic regimes. Cultures of blood and urine were sterile; but Torulopsis glabrata, a candida species, was found in multiple cultures of the sputum of all patients and also in a bronchoscopic lavage obtained from one patient after reconstitution of the granulopoesis. The roetgenologic appearance of the infiltrates was accompanied by a rise of the Ig-M immunoglobulins against candida. Following intravenous treatment with amphotericin B a reduction of the cavitation and of the infiltrates to small residues was observed. Simultaneously the body temperature and the sputum became normal and a fall in the immunoglobulin titers was found. The diagnostic problems of pulmonary cavitation and especially of pulmonary mycosis in immuosuppressed and therapy-induced granulocytopenic and thrombocytopenic patients with acute leukemia were discussed.

  8. Characterisation of microcontaminants in Darwin Harbour, a tropical estuary of northern Australia undergoing rapid development.

    Science.gov (United States)

    French, Veronica A; King, Susan Codi; Kumar, Anu; Northcott, Grant; McGuinness, Keith; Parry, David

    2015-12-01

    The detection of microcontaminants in aquatic environments raises concerns about their potential to exert ecotoxicological effects and impact human health. In contrast to freshwater habitats, little information is available on environmental concentrations in urban estuarine and marine environments. This study investigated an extensive range of organic and inorganic microcontaminants in the Darwin Harbour catchment, a tropical estuary in northern Australia undergoing rapid urbanisation and industrial development. We sampled wastewater effluent and surface water from seven sites in Darwin Harbour for pharmaceuticals and personal care products, alkylphenols, hormones, pesticides, herbicides and metals. In vitro bioassays were used to estimate the (anti)estrogenic and (anti)androgenic activities of samples. Seventy-nine of 229 organic microcontaminants analysed were detected at concentrations ranging from 0.01 to 20 μg/L, with acesulfame, paracetamol, cholesterol, caffeine, DEET and iopromide detected at the highest concentrations in wastewater effluent (20 μg/L, 17 μg/L, 11 μg/L, 11 μg/L, 10 μg/L and 7.6 μg/L, respectively). Levels of estrogenic activity ranged from estradiol equivalency quotients (EEQs) of Darwin Harbour. The measured concentration range of DEET was higher than ranges reported in previous studies. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Rapid recognition at 10 months as a predictor of language development.

    Science.gov (United States)

    Junge, Caroline; Kooijman, Valesca; Hagoort, Peter; Cutler, Anne

    2012-07-01

    Infants' ability to recognize words in continuous speech is vital for building a vocabulary. We here examined the amount and type of exposure needed for 10-month-olds to recognize words. Infants first heard a word, either embedded within an utterance or in isolation, then recognition was assessed by comparing event-related potentials to this word versus a word that they had not heard directly before. Although all 10-month-olds showed recognition responses to words first heard in isolation, not all infants showed such responses to words they had first heard within an utterance. Those that did succeed in the latter, harder, task, however, understood more words and utterances when re-tested at 12 months, and understood more words and produced more words at 24 months, compared with those who had shown no such recognition response at 10 months. The ability to rapidly recognize the words in continuous utterances is clearly linked to future language development. © 2012 Blackwell Publishing Ltd.

  10. Giant Mediastinal Cystic Teratoma Involving Pancreatic Tissue and Showing Rapid Development

    Directory of Open Access Journals (Sweden)

    Samil Gunay

    2013-10-01

    Full Text Available 23 year old female patient having increasing chest pain by breathing. In her lung graphy and computed tomography there observed 152 mm x 97mm thick encapsulated cystic lesion in the middle lobe medial part of lung that makes pressure on heart and mediastinal structures . It was also obtained that the lung graphy that had been taken a year ago was normal and a suspected view in right mediastinal region of lung graphy that had been taken 6 months ago . With the surgical operation 170 mm x 100 mm thick encapsulated cystic lesion was totally excised. There were also 5 dark brownish random tissue parts which was 10x6x2 cm as the biggest and 5x3x1 cm as the smallest in size macroscopically. During examination on tissue smples that were taken out from the bulk, there observed a pancreatic tissue with acinars, ductusus and crawlings which creats lobular structures in hyalinizing fibrotic stroma We want to present this pancreatic cystic teratoma rarely seen in mediasten which shows rapid development.

  11. Neurophysiological basis of rapid eye movement sleep behavior disorder: informing future drug development

    Directory of Open Access Journals (Sweden)

    Jennum P

    2016-04-01

    Full Text Available Poul Jennum, Julie AE Christensen, Marielle Zoetmulder Department of Clinical Neurophysiology, Faculty of Health Sciences, Danish Center for Sleep Medicine, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark Abstract: Rapid eye movement (REM sleep behavior disorder (RBD is a parasomnia characterized by a history of recurrent nocturnal dream enactment behavior and loss of skeletal muscle atonia and increased phasic muscle activity during REM sleep: REM sleep without atonia. RBD and associated comorbidities have recently been identified as one of the most specific and potentially sensitive risk factors for later development of any of the alpha-synucleinopathies: Parkinson’s disease, dementia with Lewy bodies, and other atypical parkinsonian syndromes. Several other sleep-related abnormalities have recently been identified in patients with RBD/Parkinson’s disease who experience abnormalities in sleep electroencephalographic frequencies, sleep–wake transitions, wake and sleep stability, occurrence and morphology of sleep spindles, and electrooculography measures. These findings suggest a gradual involvement of the brainstem and other structures, which is in line with the gradual involvement known in these disorders. We propose that these findings may help identify biomarkers of individuals at high risk of subsequent conversion to parkinsonism. Keywords: motor control, brain stem, hypothalamus, hypocretin

  12. Development and validation of a rapid test system for detection of pork meat and collagen residues.

    Science.gov (United States)

    Masiri, J; Benoit, L; Barrios-Lopez, B; Thienes, C; Meshgi, M; Agapov, A; Dobritsa, A; Nadala, C; Samadpour, M

    2016-11-01

    Mislabeling, contamination, and economic adulteration of meat products with undeclared pork tissues are illegal under regulations promulgated by numerous regulatory agencies. Nonetheless, analysis of the European meat industry has revealed pervasive meat adulteration, necessitating more extensive application of meat authentication testing. As existing methods for meat speciation require specialized equipment and/or training, we developed a detection system based on a lateral flow device (LFD) assay format capable of rapidly (~35min) identifying porcine residues derived from raw meat, cooked meat, and gelatin down to 0.01%, 1.0%, and 2.5% contamination, respectively. Specificity analysis revealed no cross-reactivity with meat derived from chicken, turkey, horse, beef, lamb, or goat. Comparison with a commercial ELISA kit and PCR method revealed similar if not improved sensitivity, with the added feature that the LFD-based system required considerably less time to perform. Accordingly, this test system should aid the food industry and food control authorities in monitoring for adulteration with pork. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Development of multiplex-PCR assay for rapid detection of Candida spp.

    Directory of Open Access Journals (Sweden)

    Ni Made A. Tarini

    2010-05-01

    Full Text Available Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identification of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp.Methods Five Candida spp. isolates were cultured, identifi ed with germ tube and API® 20 C AUX (BioMerieux® SA kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen® kit for Multiplex-PCR assay.Results DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of Candida spp. (Med J Indones 2010; 19:83-7Keywords: Candida spp., multiplex-PCR

  14. Development of a micropulverized extraction method for rapid toxicological analysis of methamphetamine in hair.

    Science.gov (United States)

    Miyaguchi, Hajime; Kakuta, Masaya; Iwata, Yuko T; Matsuda, Hideaki; Tazawa, Hidekatsu; Kimura, Hiroko; Inoue, Hiroyuki

    2007-09-07

    We developed a rapid sample preparation method for the toxicological analysis of methamphetamine and amphetamine (the major metabolite of methamphetamine) in human hair by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), to facilitate fast screening and quantitation. Two milligrams of hair were mechanically micropulverized for 5 min in a 2-ml plastic tube together with 100 microl of an aqueous solvent containing 10% acetonitrile, 100 mM trifluoroacetic acid and the corresponding deuterium analogues as internal standards. The pulverizing highly disintegrated the hair components, simultaneously allowing the extraction of any drugs present in the hair. After filtering the suspension with a membrane-filter unit, the clear filtrate was directly analyzed by HPLC-MS/MS. No evaporation processes were required for sample preparation. Method optimization and validation study were carried out using real-case specimens and fortified samples in which the drugs had been artificially absorbed, respectively. Concentration ranges for quantitation were 0.040-125 and 0.040-25 ng/mg for methamphetamine and amphetamine, respectively. Real-case specimens were analyzed by the method presented here and by conventional ones to verify the applicability of our method to real-world analysis. Our method took less than 30 min for a set of chromatograms to be obtained from a washed hair sample.

  15. The Central Asian Armies Facing the Challenge of Formation

    Directory of Open Access Journals (Sweden)

    Sébastien Peyrouse

    2011-03-01

    Full Text Available This article focuses on one of the main challenges that Central Asian armies face, that is, the problem of training and formation. Having rapidly increased since 2007, Central Asian military budgets have been able to multiply the purchases of equipment and weapons from foreign partners (Russia, western countries, Israel, China, South Korea, etc.. Money is not enough, however, to get the military institution back on its feet in its most human aspect, that of formation. In fact, the teaching institutions and the training possibilities provided to conscripts and professional soldiers on contract are generally of inadequate quality and impede the overall military capacities of the Central Asian states. This article will examine the main problems of the Central Asian military institutions and will discuss the means that have been implemented by Central Asian governments to reduce the negative impact of difficulties in promoting human capital.

  16. A qualitative assessment of community-based breast health navigation services for Southeast Asian women in Southern California: recommendations for developing a navigator training curriculum.

    Science.gov (United States)

    Nguyen, Tu-Uyen N; Tran, Jacqueline H; Kagawa-Singer, Marjorie; Foo, Mary Anne

    2011-01-01

    We identified key elements required for a training curriculum for Southeast Asian community-based health navigators (CBHNs), who help low-income, immigrant Cambodian, Laotian, Thai, and Vietnamese women negotiate cultural and systemic barriers to breast cancer screening and care in the United States. We gathered the perspectives of 3 groups: CBHNs, community members, and their providers. We conducted 16 focus groups with 110 women representing different stages of the cancer care continuum and in-depth interviews with 15 providers and 10 navigators to identify the essential roles, skills, and interpersonal qualities that characterize successful CBHNs. The most important areas identified for training CBHNs were information (e.g., knowing pertinent medical information and how to navigate resources), logistics (transportation, interpretation), and affective interpersonal skills (understanding the language and cultural beliefs of patients, communicating with providers, establishing trust). CBHNs serve a crucial role in building trust and making screening practices culturally meaningful, accessible, usable, and acceptable. Future research should focus on developing training curricula, policies, resources, and funding to better maximize the expertise and services that CBHNs provide and to expand our findings to other underserved communities.

  17. Life History, Reproductive Biology, and Larval Development of Ontsira mellipes (Hymenoptera: Braconidae), a Newly Associated Parasitoid of the Invasive Asian Longhorned Beetle (Coleoptera: Cerambycidae).

    Science.gov (United States)

    Golec, Julian R; Duan, Jian J; Aparicio, Ellen; Hough-Goldstein, Judith

    2016-08-01

    The invasive Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), is a destructive xylophagous forest pest species originating from Asia. Several endemic North American hymenopteran (Braconidae) species in the mid-Atlantic region were capable of attacking and reproducing on A. glabripennis larvae in laboratory bioassays. Ontsira mellipes Ashmead (Hymenoptera: Braconidae) has been continually reared on A. glabripennis larvae at USDA-ARS BIIRU since 2010, and has been identified as a potential new-association biocontrol agent. Two experiments were conducted to investigate parasitism, paralysis, reproductive biology, larval development, and longevity of adult O. mellipes In the first experiment, pairs of adult parasitoids were given single A. glabripennis larvae every 2 d (along with honey and water) over their lifetimes, while in the second experiment individual parasitoids were observed daily from egg to adult, and adults were subsequently starved. Adults in the first experiment parasitized ∼21% of beetle larvae presented to them throughout their life, and paralysis of larvae occurred 1-2 d after oviposition. More than half of the individual pairs parasitized A. glabripennis larvae, with each female producing around 26 offspring throughout her life. In the second experiment, median development time of O. mellipes from egg to adult was about 3 wk, with five larval instars. Adult O. mellipes that were provided with host larvae, honey, and water lived 9 d longer than host-deprived and starved adults. These findings indicate that mass-rearing procedures for O. mellipes may be developed using the new association host for development of effective biocontrol programs against A. glabripennis. Published by Oxford University Press on behalf of Entomological Society of America 2016. This work is written by US Government employees and is in the public domain in the United States.

  18. Asian Art on Display

    DEFF Research Database (Denmark)

    Borggreen, Gunhild Ravn

    2010-01-01

    Med udgangspunkt i seminaret Visualising Asian Modernity diskuteres forholdet mellem antropologi og samtidskunst i lyset af hvorledes asiatisk kunst fremvises og formidles i vestlig og dansk sammenhæng....

  19. Profile: Asian Americans

    Science.gov (United States)

    ... and diabetes. Asian Americans also have a high prevalence of the following conditions and risk factors: chronic obstructive pulmonary disease, hepatitis B, HIV/AIDS, smoking, tuberculosis, and liver ...

  20. The South Asian genome.

    Directory of Open Access Journals (Sweden)

    John C Chambers

    Full Text Available The genetic sequence variation of people from the Indian subcontinent who comprise one-quarter of the world's population, is not well described. We carried out whole genome sequencing of 168 South Asians, along with whole-exome sequencing of 147 South Asians to provide deeper characterisation of coding regions. We identify 12,962,155 autosomal sequence variants, including 2,946,861 new SNPs and 312,738 novel indels. This catalogue of SNPs and indels amongst South Asians provides the first comprehensive map of genetic variation in this major human population, and reveals evidence for selective pressures on genes involved in skin biology, metabolism, infection and immunity. Our results will accelerate the search for the genetic variants underlying susceptibility to disorders such as type-2 diabetes and cardiovascular disease which are highly prevalent amongst South Asians.

  1. Assessing health systems for type 1 diabetes in sub-Saharan Africa: developing a 'Rapid Assessment Protocol for Insulin Access'

    DEFF Research Database (Denmark)

    Beran, David; Yudkin, John S; de Courten, Maximilian

    2006-01-01

    In order to improve the health of people with Type 1 diabetes in developing countries, a clear analysis of the constraints to insulin access and diabetes care is needed. We developed a Rapid Assessment Protocol for Insulin Access, comprising a series of questionnaires as well as a protocol for th...

  2. Undergraduate medical education: tendencies and requirements in a rapidly developing Europe.

    Science.gov (United States)

    Breipohl, W; Johansson, C; Hansis, M; Steiger, J; Naguro, T; Müller, K; Mestres, P

    2000-01-01

    This study pinpoints the necessity to constantly monitor local approaches in undergraduate medical education on an inter-European scale. Traditional undergraduate medical curricula need restructuring to account for the increasing amount of medical knowledge and rapid changes and developments in societies, nosology, therapy and IT. European undergraduate medical curricula should be harmonized not egalized, with a focus on inter-European sharing of resources, mobility, credit (allocation, accumulation and transfer), definition of European and trans-European mission statements, identification of quality metrics, advice on dealing with conflicting aims such as specialization and generalization, on communicating core knowledge instead of providing overabundance of information, and on introducing multifaceted teaching and learning methods, as well as providing strategies for life long learning. Sound medical education can no longer and nowhere be considered under the autonomous auspices of individual Medical Schools or national philosophies. It has to be perceived and structured as a competitive and flexible approach which promotes life long learning of teachers, students, physicians and other related staff with international awareness. It is stressed that student and staff mobility, as well as virtual mobility in the form of worldwide available teaching modules and expertise have to be incorporated into national medical curricula. This is to guarantee up-to-date education in support of patient demands, future professionality and competitiveness of students, physicians and Public Health System institutions. The formal approaches of traditional subject related curricula as well as problem based learning must be linked with quality approved state of the art ODL, evaluated international CME strategies and training in the utilization of IT in preparation of lifelong learning. Strategies for the use of IT need updating on a regular basis to diminish the gap between

  3. Development of rapid methods for relaxation time mapping and motion estimation using magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Gilani, Syed Irtiza Ali

    2008-09-15

    Recent technological developments in the field of magnetic resonance imaging have resulted in advanced techniques that can reduce the total time to acquire images. For applications such as relaxation time mapping, which enables improved visualisation of in vivo structures, rapid imaging techniques are highly desirable. TAPIR is a Look- Locker-based sequence for high-resolution, multislice T{sub 1} relaxation time mapping. Despite the high accuracy and precision of TAPIR, an improvement in the k-space sampling trajectory is desired to acquire data in clinically acceptable times. In this thesis, a new trajectory, termed line-sharing, is introduced for TAPIR that can potentially reduce the acquisition time by 40 %. Additionally, the line-sharing method was compared with the GRAPPA parallel imaging method. These methods were employed to reconstruct time-point images from the data acquired on a 4T high-field MR research scanner. Multislice, multipoint in vivo results obtained using these methods are presented. Despite improvement in acquisition speed, through line-sharing, for example, motion remains a problem and artefact-free data cannot always be obtained. Therefore, in this thesis, a rapid technique is introduced to estimate in-plane motion. The presented technique is based on calculating the in-plane motion parameters, i.e., translation and rotation, by registering the low-resolution MR images. The rotation estimation method is based on the pseudo-polar FFT, where the Fourier domain is composed of frequencies that reside in an oversampled set of non-angularly, equispaced points. The essence of the method is that unlike other Fourier-based registration schemes, the employed approach does not require any interpolation to calculate the pseudo-polar FFT grid coordinates. Translation parameters are estimated by the phase correlation method. However, instead of two-dimensional analysis of the phase correlation matrix, a low complexity subspace identification of the phase

  4. Development of an isothermal amplification-based assay for the rapid visual detection of Salmonella bacteria.

    Science.gov (United States)

    Liu, Hai-Bin; Zang, Yu-Xuan; Du, Xin-Jun; Li, Ping; Wang, Shuo

    2017-09-01

    The efficient and timely detection of pathogens is a major concern worldwide. The aim of this study was to establish a rapid detection method for Salmonella bacteria in food samples to facilitate timely treatment. Widely used detection methods currently include culture-based methods and PCR-based methods. The former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. In this study, a sensitive and rapid approach for the visual and point-of-use detection of Salmonella bacteria based on recombinase polymerase amplification (RPA) and a lateral-flow (LF) nucleic acid strip was established. We designed a pair of primers according to the invA gene of Salmonella bacteria: one was modified with digoxin, and the other was modified with biotin. In the presence of the biotin- and digoxin-modified primers and target DNA, the RPA produced a substantial amount of duplex DNA attached to biotin and digoxin. The products were detected using LF strips through immunoreaction: anti-digoxin antibodies on the gold nanoparticles, digoxin on the duplex, streptavidin on the LF test line, and biotin on the duplex. The developed RPA-LF assay allowed detection of Salmonella genomic DNA in less than 20 min with simple water bath equipment or portable thermal equipment. In addition, the RPA-LF assay was highly sensitive, with a detection limit as low as 20 fg of target DNA or 1.05 × 101 cfu of bacteria in pure culture, and highly specific, exhibiting no cross-reaction with Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Shigella, Enterobacter aerogenes, or Campylobacter jejuni. Importantly, Salmonella could be detected in milk and chicken breast at concentrations as low as 1.05 × 100 cfu/mL or 1.05 × 100 cfu/g after enrichment for 2 h and in eggs at 1.05 × 100 cfu/g after enrichment for 4 h. Furthermore, RPA was more sensitive than PCR, which requires a thermal cycling

  5. The Asian Face Lift

    OpenAIRE

    Bergeron, Léonard; Chen, Yu-Ray

    2009-01-01

    The face-lift procedure (rhytidectomy) is increasingly popular in Asia. There is extensive literature on different techniques in Western patients. Cultural and anthropomorphologic differences between Asian and Caucasians require the adaptation of current techniques to obtain a satisfactory outcome for both the patient and the surgeon. This article therefore attempts to define important differences between Asians and Caucasians in terms of signs of facial aging, perception of beauty, and surgi...

  6. Rape: an Asian perspective.

    Science.gov (United States)

    Nadesan, K

    2001-06-01

    Rape is one of the fastest growing violent crimes in many parts of the world. Rape laws have been amended in most countries in an attempt to cope with the proliferation of this crime. Even though the legal definition of rape and the procedural laws have been amended, rape remains a serious problem in both the developed and developing nations. In some countries the offence of rape carries severe punishment sometimes even the death sentence. In many jurisdictions the term 'sexual penetration' is being used instead of 'sexual intercourse'. Sexual penetration includes sexual intercourse, anal intercourse, cunnilingus, fellatio or any other intrusions involving any part of a human body or of any object into the genital or anal opening of a person's body. In many countries rape and other sexual offences have been replaced with a series of gender neutral and graded offences with appropriate punishments. Medical examination can provide independent, scientific, corroborative evidence that may be of value to the court in arriving at a judgement. Doctors should have a clear understanding of different rape laws in order to apprectiate the various issues involved. Special knowledge, skill and experience are essential to conduct a good-quality medical examination. There is a dearth of trained forensic physicians in many Asian countries. However, managing a rape victim (survivor) goes for beyond proving the case in a court of law. There should be an adequate rehabilitation programme available to the victims to help them cope.

  7. Software Toolbox Development for Rapid Earthquake Source Optimisation Combining InSAR Data and Seismic Waveforms

    Science.gov (United States)

    Isken, Marius P.; Sudhaus, Henriette; Heimann, Sebastian; Steinberg, Andreas; Bathke, Hannes M.

    2017-04-01

    We present a modular open-source software framework (pyrocko, kite, grond; http://pyrocko.org) for rapid InSAR data post-processing and modelling of tectonic and volcanic displacement fields derived from satellite data. Our aim is to ease and streamline the joint optimisation of earthquake observations from InSAR and GPS data together with seismological waveforms for an improved estimation of the ruptures' parameters. Through this approach we can provide finite models of earthquake ruptures and therefore contribute to a timely and better understanding of earthquake kinematics. The new kite module enables a fast processing of unwrapped InSAR scenes for source modelling: the spatial sub-sampling and data error/noise estimation for the interferogram is evaluated automatically and interactively. The rupture's near-field surface displacement data are then combined with seismic far-field waveforms and jointly modelled using the pyrocko.gf framwork, which allows for fast forward modelling based on pre-calculated elastodynamic and elastostatic Green's functions. Lastly the grond module supplies a bootstrap-based probabilistic (Monte Carlo) joint optimisation to estimate the parameters and uncertainties of a finite-source earthquake rupture model. We describe the developed and applied methods as an effort to establish a semi-automatic processing and modelling chain. The framework is applied to Sentinel-1 data from the 2016 Central Italy earthquake sequence, where we present the earthquake mechanism and rupture model from which we derive regions of increased coulomb stress. The open source software framework is developed at GFZ Potsdam and at the University of Kiel, Germany, it is written in Python and C programming languages. The toolbox architecture is modular and independent, and can be utilized flexibly for a variety of geophysical problems. This work is conducted within the BridGeS project (http://www.bridges.uni-kiel.de) funded by the German Research Foundation DFG

  8. Colon cancer in rapidly developing countries: review of the lifestyle, dietary, consanguinity and hereditary risk factors

    Directory of Open Access Journals (Sweden)

    Abdulbari Bener

    2011-10-01

    Full Text Available Colon cancer rates are rising dramatically in once low incidence nations. These nations are undergoing rapid economic development and are known as “nations in transition” (NIT. This review identifies some of the most common etiological risk factors of colon cancer in these nations and evaluates the existing epidemiological evidence. The main risk factors which were found to be prevalent in NIT include: lifestyle factors such as physical inactivity, obesity and abdominal adiposity, alcohol consumption and cigarette smoking; dietary factors such as fatty food and red meat consumption. Protective factors included white meat and fiber consumption. Several studies found to have significantly higher rates of colon cancer among the young population (<40 years old. There appears to be a quantitative and qualitative increase in risk to relatives of patients diagnosed at a young age compared with those diagnosed later in life, at least part of which is likely to be the result of a hereditary susceptibility. Close relatives of patients with colon cancer are at an increased risk of developing a colon cancer. Close relatives of early onset cases warrant more intensive endoscopic screening and at an earlier age than relatives of patients diagnosed at older ages. Furthermore, these suggest the existence of genetic predispositions in these nations which need to be investigated further and have implications for screening programs. In conclusion, public health awareness campaigns promoting prevention of modifiable risk factors and screening initiatives with guidelines suited to the age-specific incidence rates of NIT are needed very urgently.

  9. Development of a stress scale for pregnant women in the South Asian context: the A-Z Stress Scale.

    Science.gov (United States)

    Kazi, A; Fatmi, Z; Hatcher, J; Niaz, U; Aziz, A

    2009-01-01

    Stress in pregnancy can lead to low-birth-weight and preterm babies and to psychological consequences such as anxiety and depression during pregnancy and the puerperium. Previous scales to measure stress contain items that overlap with the symptoms of pregnancy. A stress scale was developed based on in-depth interviews with pregnant women in Pakistan. Construct validity, test-retest reliability and inter-rater reliability were carried out. Cronbach alpha was 0.82 for the 30 short-listed items, with item-total correlations of 0.2-0.8. Multidimensional scaling determined 2 dimensions: socioenvironmental hassles and chronic illnesses. This was the first scale developed for pregnant women based on stressors in a developing country in South Asia.

  10. Holographic Spectroscopy for Rapid Electron Bunch Analysis: Development of an Instrument with THZ Resolved Optical Gating

    Energy Technology Data Exchange (ETDEWEB)

    Sievers, Albert

    2011-10-28

    radiation and provided the first step in the development of a self contained THz HFTS for rapid electron bunch shape analysis. Our first experiments confirmed the idea and as a side result made possible a completely new bunch length monitor eliminating any need for an external laser since only radiation produced by the bunch itself is involved. A more advanced instrument was designed to use the incoherent visible radiation pulse from the synchrotron source for sampling the THz interference pattern. It has been built and the first tests are to be conducted in April 2011 at the Jefferson FEL facility.

  11. Rapid HIV testing for developing countries: the challenge of false-negative tests

    Science.gov (United States)

    Yogev, Ram

    2012-06-01

    It is a common practice in resource-constrained countries to accept two positive rapid HIV antibody test results as diagnostic for HIV infection. Because these tests are inexpensive and results are obtained quickly, they are recommended by the WHO to "scale-up" HIV testing to increase the number of people tested. The negative predictive value of rapid HIV tests is so high that negative results are considered conclusive despite the fact that false-negative results can occur in several situations. While the specificity and sensitivity of rapid HIV tests in resource-rich countries is acceptable, there are only limited data about their performance in resource-constrained countries. The challenges of rapid HIV testing in these situations will be discussed.

  12. Private Investment in Power Sector in Developing Countries: Lessons from Reforms in Asian and Latin American Countries.

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Anoop

    2007-07-01

    The demand for investment in the electricity sector in the world between 2000 and 2030 is estimated to be USD9.8 trillion. Developing countries would require more than half of this investment. Given the limited fiscal space for public investment, a number of developing countries have undertaken policy initiatives to improve the investment climate for the private sector. The pace, scope, sequencing and outcome of reform process varies across countries. This paper undertakes a comparative evaluation of policy and regulatory reforms in Argentina, Brazil, India, Mexico, PRC and Thailand. The paper concludes that apart from macroeconomic stability, the pace and sequencing of reforms influences private investment in the power sector. Distribution reforms and setting up of an independent regulatory institution reduces risk for investors. A peace-meal approach to reform keeps uncertainties alive for investors and does not translate in significant investment in the sector. (auth)

  13. [Embryonic and Larval Development of the Asian Seabass Lates calcarifer (Pisces: Perciformes: Latidae) under Thermostatically Controlled Conditions].

    Science.gov (United States)

    Shadrin, A M; Pavlov, D S

    2015-01-01

    Material for this study was obtained from the hatchery with brood stock of Lates calcarifer that originated from a natural population living in inshore waters off Central Vietnam. Commercial interest in L. calcarifer as an object of mariculture and wildstock fishery has resulted in several publications on its early life history; nevertheless, comprehensive description of early development of L. calcarifer based on controlled incubation of embryos and larvae has remained absent. In the present paper embryonic and larval development to the stage of anlage of pelvic fins is described in detail and illustrated with original drawings of live material on the basis of thermostatically controlled incubation of embryos at 27°C and larvae at 26.8°C (26.5-28.0°C). The first cleavage furrow appeared at the age of 33.5 min. The duration of synchronous cleavage cycle was 16 min. About 80% of all embryos hatched at the age of 18 h. The length of newly hatched larva during the first hour after emergence from the egg shell was 1.63 ± 0.016 mm (1.50-1.75 mm). Chronology of development of the organs, early circulatory system, and pigmentation pattern is given. The dynamics of change in the trunk and caudal body segment number in larva from hatching to the moment of anlage of pelvic fins is shown. The total number of body segments reached the maximum value of 26-27 soon after hatching and then decreased to 20-21 segments. Newly received data are discussed in a comparative context of development of some other teleosts.

  14. Development of a rapid method for the quantitative determination of deoxynivalenol using Quenchbody

    Energy Technology Data Exchange (ETDEWEB)

    Yoshinari, Tomoya [Division of Microbiology, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501 (Japan); Ohashi, Hiroyuki; Abe, Ryoji; Kaigome, Rena [Biomedical Division, Ushio Inc., 1-12 Minamiwatarida-cho, Kawasaki-ku, Kawasaki 210-0855 (Japan); Ohkawa, Hideo [Research Center for Environmental Genomics, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501 (Japan); Sugita-Konishi, Yoshiko, E-mail: y-konishi@azabu-u.ac.jp [Department of Food and Life Science, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201 (Japan)

    2015-08-12

    Quenchbody (Q-body) is a novel fluorescent biosensor based on the antigen-dependent removal of a quenching effect on a fluorophore attached to antibody domains. In order to develop a method using Q-body for the quantitative determination of deoxynivalenol (DON), a trichothecene mycotoxin produced by some Fusarium species, anti-DON Q-body was synthesized from the sequence information of a monoclonal antibody specific to DON. When the purified anti-DON Q-body was mixed with DON, a dose-dependent increase in the fluorescence intensity was observed and the detection range was between 0.0003 and 3 mg L{sup −1}. The coefficients of variation were 7.9% at 0.003 mg L{sup −1}, 5.0% at 0.03 mg L{sup −1} and 13.7% at 0.3 mg L{sup −1}, respectively. The limit of detection was 0.006 mg L{sup −1} for DON in wheat. The Q-body showed an antigen-dependent fluorescence enhancement even in the presence of wheat extracts. To validate the analytical method using Q-body, a spike-and-recovery experiment was performed using four spiked wheat samples. The recoveries were in the range of 94.9–100.2%. The concentrations of DON in twenty-one naturally contaminated wheat samples were quantitated by the Q-body method, LC-MS/MS and an immunochromatographic assay kit. The LC-MS/MS analysis showed that the levels of DON contamination in the samples were between 0.001 and 2.68 mg kg{sup −1}. The concentrations of DON quantitated by LC-MS/MS were more strongly correlated with those using the Q-body method (R{sup 2} = 0.9760) than the immunochromatographic assay kit (R{sup 2} = 0.8824). These data indicate that the Q-body system for the determination of DON in wheat samples was successfully developed and Q-body is expected to have a range of applications in the field of food safety. - Highlights: • A rapid method for quantitation of DON using Q-body has been developed. • A recovery test using the anti-DON Q-body was performed. • The concentrations of DON in wheat

  15. Planar array stack design aided by rapid prototyping in development of air-breathing PEMFC

    Science.gov (United States)

    Chen, Chen-Yu; Lai, Wei-Hsiang; Weng, Biing-Jyh; Chuang, Huey-Jan; Hsieh, Ching-Yuan; Kung, Chien-Chih

    The polymer electrolyte membrane fuel cell (PEMFC) is one of the most important research topics in the new and clean energy area. The middle or high power PEMFCs can be applied to the transportation or the distributed power system. But for the small power application, it is needed to match the power requirement of the product generally. On the other hand, the direct methanol fuel cell (DMFC) is one of the most common type that researchers are interested in, but recently the miniature or the micro-PEMFCs attract more attention due to their advantages of high open circuit voltage and high power density. The objective of this study is to develop a new air-breathing planar array fuel cell stacked from 10 cells made by rapid prototyping technology which has potential for fast commercial design, low cost manufacturing, and even without converters/inverters for the system. In this paper, the main material of flow field plates is acrylonitrile-butadiene-styrene (ABS) which allows the fuel cell be mass-manufactured by plastic injection molding technology. The rapid prototyping technology is applied to construct the prototype and verify the practicability of the proposed stack design. A 10-cell air-breathing miniature PEMFC stack with a volume of 6 cm × 6 cm × 0.9 cm is developed and tested. Its segmented membrane electrode assembly (MEA) is designed with the active surface area of 1.3 cm × 1.3 cm in each individual MEA. The platinum loading at anode and cathode are 0.2 mg cm -2 and 0.4 mg cm -2, respectively. Results show that the peak power densities of the parallel connected and serial connected stack are 99 mW cm -2 at 0.425 V and 92 mW cm -2 at 4.25 V, respectively under the conditions of 70 °C relative saturated humidity (i.e., dew point temperature), ambient temperature and free convection air. Besides, the stack performance is increased under forced convection. If the cell surface air is blown by an electric fan, the peak power densities of parallel connected and

  16. Planar array stack design aided by rapid prototyping in development of air-breathing PEMFC

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Chen-Yu.; Lai, Wei-Hsiang [Institute of Aeronautics and Astronautics, National Cheng Kung University, No.1, Ta-Hsueh Road, Tainan 701 (China); Weng, Biing-Jyh; Chuang, Huey-Jan; Hsieh, Ching-Yuan; Kung, Chien-Chih [Chung-Shan Institute of Science and Technology, Materials and Electro-Optics Research Division, P.O. Box No. 90008-8-3 Lung-Tan, Tao-Yuan 325 (China)

    2008-04-15

    The polymer electrolyte membrane fuel cell (PEMFC) is one of the most important research topics in the new and clean energy area. The middle or high power PEMFCs can be applied to the transportation or the distributed power system. But for the small power application, it is needed to match the power requirement of the product generally. On the other hand, the direct methanol fuel cell (DMFC) is one of the most common type that researchers are interested in, but recently the miniature or the micro-PEMFCs attract more attention due to their advantages of high open circuit voltage and high power density. The objective of this study is to develop a new air-breathing planar array fuel cell stacked from 10 cells made by rapid prototyping technology which has potential for fast commercial design, low cost manufacturing, and even without converters/inverters for the system. In this paper, the main material of flow field plates is acrylonitrile-butadiene-styrene (ABS) which allows the fuel cell be mass-manufactured by plastic injection molding technology. The rapid prototyping technology is applied to construct the prototype and verify the practicability of the proposed stack design. A 10-cell air-breathing miniature PEMFC stack with a volume of 6 cm x 6 cm x 0.9 cm is developed and tested. Its segmented membrane electrode assembly (MEA) is designed with the active surface area of 1.3 cm x 1.3 cm in each individual MEA. The platinum loading at anode and cathode are 0.2 mg cm{sup -2} and 0.4 mg cm{sup -2}, respectively. Results show that the peak power densities of the parallel connected and serial connected stack are 99 mW cm{sup -2} at 0.425 V and 92 mW cm{sup -2} at 4.25 V, respectively under the conditions of 70 C relative saturated humidity (i.e., dew point temperature), ambient temperature and free convection air. Besides, the stack performance is increased under forced convection. If the cell surface air is blown by an electric fan, the peak power densities of

  17. Development of macroporous calcium phosphate scaffold processed via microwave rapid drying

    Energy Technology Data Exchange (ETDEWEB)

    Jamuna-Thevi, K., E-mail: jamuna@sirim.my [Advanced Materials Research Centre (AMREC), SIRIM Berhad, Lot 34, Jalan Hi-Tech 2/3, Kulim Hi-Tech Park, 09000 Kulim, Kedah (Malaysia); Zakaria, F.A. [Advanced Materials Research Centre (AMREC), SIRIM Berhad, Lot 34, Jalan Hi-Tech 2/3, Kulim Hi-Tech Park, 09000 Kulim, Kedah (Malaysia); Othman, R. [Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, Seri Ampangan, 14300 Nibong Tebal, Penang (Malaysia); Muhamad, S. [Bioassay Unit, Herbal Medicine Research Centre (HMRC), Institute for Medical Research (IMR), Jalan Pahang, 50588 Kuala Lumpur (Malaysia)

    2009-06-01

    Porous hydroxyapatite (HA) scaffold has great potential in bone tissue engineering applications. A new method to fabricate macroporous calcium phosphate (CP) scaffold via microwave irradiation, followed by conventional sintering to form HA scaffold was developed. Incorporation of trisodium citrate dihydrate and citric acid in the CP mixture gave macroporous scaffolds upon microwave rapid drying. In this work, a mixture of {beta}-tricalcium phosphate ({beta}-TCP), calcium carbonate (CaCO{sub 3}), trisodium citrate dihydrate, citric acid and double distilled de-ionised water (DDI) was exposed to microwave radiation to form a macroporous structure. Based on gross eye examinations, addition of trisodium citrate at 30 and 40 wt.% in the CP mixture ({beta}-TCP and CaCO{sub 3}) without citric acid indicates increasing order of pore volume where the highest porosity yield was observed at 40 wt.% of trisodium citrate addition and the pore size was detected at several millimeters. Therefore, optimization of pore size was performed by adding 3-7 wt.% of citric acid in the CP mixture which was separately mixed with 30 and 40 wt.% of trisodium citrate for comparison purposes. Fabricated scaffolds were calcined at 600 deg. C and washed with DDI water to remove the sodium hydroxycarbonate and sintered at 1250 deg. C to form HA phase as confirmed in the X-ray diffraction (XRD) results. Based on Archimedes method, HA scaffolds prepared from 40 wt.% of trisodium citrate with 3-7 wt.% of citric acid added CP mixture have an open and interconnected porous structure ranging from 51 to 53 vol.% and observation using Scanning electron microscope (SEM) showed the pore size distribution between 100 and 500 {mu}m. The cytotoxicity tests revealed that the porous HA scaffolds have no cytotoxic potential on MG63 osteoblast-like cells which might allow for their use as biomaterials.

  18. Using Simulation to Develop Care Models for Rapid Response and Code Teams at a Satellite Facility.

    Science.gov (United States)

    Rule, Amy R L; Snider, Julie; Marshall, Cheryl; Kramer, Kathleen; Geis, Gary L; Tegtmeyer, Ken; Gosdin, Craig H

    2017-12-01

    Our institution recently completed an expansion of an acute care inpatient unit within a satellite hospital that does not include an on-site ICU or PICU. Because of expected increases in volume and acuity, new care models for Rapid Response Teams (RRTs) and Code Blue Teams were necessary. Using simulation-based training, our objectives were to define the optimal roles and responsibilities for team members (including ICU physicians via telemedicine), refine the staffing of RRTs and code Teams, and identify latent safety threats (LSTs) before opening the expanded inpatient unit. The laboratory-based intervention consisted of 8 scenarios anticipated to occur at the new campus, with each simulation followed by an iterative debriefing process and a 30-minute safety talk delivered within 4-hour interprofessional sessions. In situ sessions were delivered after construction and before patients were admitted. A total of 175 clinicians completed a 4-hour course in 17 sessions. Over 60 clinicians participated during 2 in situ sessions before the opening of the unit. Eleven team-level knowledge deficits, 19 LSTs, and 25 system-level issues were identified, which directly informed changes and refinements in care models at the bedside and via telemedicine consultation. Simulation-based training can assist in developing staffing models, refining the RRT and code processes, and identify LSTs in a new pediatric acute care unit. This training model could be used as a template for other facilities looking to expand pediatric acute care at outlying smaller, more resource-limited facilities to evaluate new teams and environments before patient exposure. Copyright © 2017 by the American Academy of Pediatrics.

  19. Development of a loop-mediated isothermal amplification assay for rapid detection of capripoxviruses.

    Science.gov (United States)

    Das, Amaresh; Babiuk, Shawn; McIntosh, Michael T

    2012-05-01

    Sheep pox (SP), goat pox (GP), and lumpy skin disease (LSD), caused by capripoxviruses (CaPVs), are economically important diseases of sheep, goats, and cattle, respectively. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of CaPVs. LAMP primers were designed to target a conserved gene encoding the poly(A) polymerase small subunit (VP39) of CaPVs. Hydroxynaphthol blue (HNB) was incorporated to monitor assay progress by color change from violet when negative to sky blue when positive, and results were verified by agarose gel electrophoresis. The LAMP assay was shown to be highly specific for CaPVs, with no apparent cross-reactivity to other related viruses (near neighbors) or viruses that cause similar clinical signs (look-a-like viruses). The performance of LAMP was compared to that of a highly sensitive quantitative real-time PCR (qPCR) assay. LAMP and qPCR exhibited similar analytical sensitivities, with limits of detection of 3 and 8 viral genome copies, respectively. Diagnostic specificity was assessed on 36 negative specimens, including swabs and EDTA blood from control sheep, goats, and cattle. Diagnostic sensitivity was assessed on 275 specimens, including EDTA blood, swabs, and tissues from experimentally infected sheep, goats, and cattle. Overall agreement on diagnostic test results between the two assays was 90 to 95% for specificity and 89 to 100% for sensitivity. The LAMP assay described in this report is simple to use, inexpensive, highly sensitive, and particularly well suited for the diagnosis of capripox in less well equipped laboratories and in rural settings where resources are limited.

  20. Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis

    Science.gov (United States)

    Peters, Sarah E.; Wang, Jinhong; Hernandez-Garcia, Juan; Weinert, Lucy A.; Luan, Shi-Lu; Chaudhuri, Roy R.; Angen, Øystein; Aragon, Virginia; Williamson, Susanna M.; Langford, Paul R.; Rycroft, Andrew N.; Wren, Brendan W.; Maskell, Duncan J.; Tucker, Alexander W.

    2015-01-01

    Haemophilus parasuis causes Glässer's disease and pneumonia in pigs. Indirect hemagglutination (IHA) is typically used to serotype this bacterium, distinguishing 15 serovars with some nontypeable isolates. The capsule loci of the 15 reference strains have been annotated, and significant genetic variation was identified between serovars, with the exception of serovars 5 and 12. A capsule locus and in silico serovar were identified for all but two nontypeable isolates in our collection of >200 isolates. Here, we describe the development of a multiplex PCR, based on variation within the capsule loci of the 15 serovars of H. parasuis, for rapid molecular serotyping. The multiplex PCR (mPCR) distinguished between all previously described serovars except 5 and 12, which were detected by the same pair of primers. The detection limit of the mPCR was 4.29 × 105 ng/μl bacterial genomic DNA, and high specificity was indicated by the absence of reactivity against closely related commensal Pasteurellaceae and other bacterial pathogens of pigs. A subset of 150 isolates from a previously sequenced H. parasuis collection was used to validate the mPCR with 100% accuracy compared to the in silico results. In addition, the two in silico-nontypeable isolates were typeable using the mPCR. A further 84 isolates were analyzed by mPCR and compared to the IHA serotyping results with 90% concordance (excluding those that were nontypeable by IHA). The mPCR was faster, more sensitive, and more specific than IHA, enabling the differentiation of 14 of the 15 serovars of H. parasuis. PMID:26424843

  1. Rapid development of cyanobacterial crust in the field for combating desertification.

    Science.gov (United States)

    Park, Chan-Ho; Li, Xin Rong; Zhao, Yang; Jia, Rong Liang; Hur, Jae-Seoun

    2017-01-01

    Desertification is currently a major concern, and vast regions have already been devastated in the arid zones of many countries. Combined application of cyanobacteria with soil fixing chemicals is a novel method of restoring desertified areas. Three cyanobacteria, Nostoc sp. Vaucher ex Bornet & Flahault, Phormidium sp. Kützing ex Gomont and Scytonema arcangeli Bornet ex Flahault were isolated and tested in this study. Tacki-SprayTM (TKS7), which consists of bio-polysaccharides and tackifiers, was used as a soil fixing agent. In addition, superabsorbent polymer (SAP) was applied to the soil as a water-holding material and nutrient supplement. Application of cyanobacteria with superabsorbent polymer and TKS7 (CST) remarkably improved macro-aggregate stability against water and erodibility against wind after 12 months of inoculation when compared to the control soil. The mean weight diameter and threshold friction velocity of the CST treated soil were found to be 75% and 88% of those of the approximately 20-year-old natural cyanobacterial crust (N-BSC), respectively, while these values were 68% and 73% of those of the N-BSC soil after a single treatment of cyanobacteria alone (CY). Interestingly, biological activities of CST were similar to those of CY. Total carbohydrate contents, cyanobacterial biomass, microbial biomass, soil respiration, carbon fixation and effective quantum yield of CST treated soil were enhanced by 50-100% of the N-BSC, while those of control soil were negligible. Our results suggest that combined application of cyanobacteria with soil fixing chemicals can rapidly develop cyanobacterial crust formation in the field within 12 months. The physical properties and biological activities of the inoculated cyanobacterial crust were stable during the study period. The novel method presented herein serves as another approach for combating desertification in arid regions.

  2. Continuing Professional Development Evaluation: Two Rapid Review Courses inNephrology and Rheumatology

    Directory of Open Access Journals (Sweden)

    Abdullah Shehab

    2012-09-01

    Full Text Available Objectives: Continuing professional development (CPD is anovel approach to increase professional knowledge and skills. The aim of this study is to explore participants’ characteristics and to understand participants’ views on two rapid review courses (RRCs as part of CPD program, and to assess healthcare providers’ views about the use of internet for accessing medical information.Methods: Data were collected from 150 participants who attended an RRC in Nephrology and Rheumatology as part of an ongoing CME program.Results: Participants’ response rate was 92% and 84.4% in Nephrology and Rheumatology RRCs, respectively. Participants’ Mean Age±SD were 39±2.1 and 41±2.1 years in the Nephrology and Rheumatology courses, respectively. Demographic variables, i.e., age, gender, and specialization showed a significant (p<0.01 impact on the learning objectives of the program. Further, participants reported that the course material had a significant (p<0.02 impacton their knowledge. Finding new medical information was the primary motive to search the internet among all participants. About half of the subjects reported knowledge of their preferred medical education sites and had access at their clinical setup. Barriers to internet use included lack of specific information, difficulty to download contents, and excessive material. Professional association websites, online journals, and CME programs were the most frequently searched sources of information. Most of the subjects reported significant (p<0.02 barriers to find medical resources on the internet and to adequately utilize the currently available medical search engines available in the healthcare system.Conclusion: A discipline specific and integrated CPD programmay have provided dual benefit such as accredited CME hours and a significant change in the participants’ knowledge. There is a need to increase Internet accessibility and capacity in the current healthcare facilities. Future CPD

  3. Activation of Notch3 in Glomeruli Promotes the Development of Rapidly Progressive Renal Disease.

    Science.gov (United States)

    El Machhour, Fala; Keuylian, Zela; Kavvadas, Panagiotis; Dussaule, Jean-Claude; Chatziantoniou, Christos

    2015-07-01

    Notch3 expression is found in the glomerular podocytes of patients with lupus nephritis or focal segmental GN but not in normal kidneys. Here, we show that activation of the Notch3 receptor in the glomeruli is a turning point inducing phenotypic changes in podocytes promoting renal inflammation and fibrosis and leading to disease progression. In a model of rapidly progressive GN, Notch3 expression was induced by several-fold in podocytes concurrently with disease progression. By contrast, mice lacking Notch3 expression were protected because they exhibited less proteinuria, uremia, and inflammatory infiltration. Podocyte outgrowth from glomeruli isolated from wild-type mice during the early phase of the disease was higher than outgrowth from glomeruli of mice lacking Notch3. In vitro studies confirmed that podocytes expressing active Notch3 reorganize their cytoskeleton toward a proliferative/migratory and inflammatory phenotype. We then administered antisense oligodeoxynucleotides targeting Notch3 or scramble control oligodeoxynucleotides in wild-type mice concomitant to disease induction. Both groups developed chronic renal disease, but mice injected with Notch3 antisense had lower values of plasma urea and proteinuria and inflammatory infiltration. The improvement of renal function was accompanied by fewer deposits of fibrin within the glomeruli and by decreased peritubular inflammation. Finally, abnormal Notch3 staining was observed in biopsy samples of patients with crescentic GN. These results demonstrate that abnormal activation of Notch3 may be involved in the progression of renal disease by promoting migratory and proinflammatory pathways. Inhibiting Notch3 activation could be a novel, promising approach to treat GN. Copyright © 2015 by the American Society of Nephrology.

  4. Rapid development of tissue bank achieved by International Atomic Energy Agency (IAEA) Tissue Banking Programme in China.

    Science.gov (United States)

    Zhang, Yu-Min; Wang, Jian-Ru; Zhang, Nai-Li; Liu, Xiao-Ming; Zhou, Mo; Ma, Shao-Ying; Yang, Ting; Li, Bao-Xing

    2014-09-01

    Before 1986, the development of tissue banking in China has been slow and relatively uncoordinated. Under the support of International Atomic Energy Agency (IAEA), Tissue Banking in China experienced rapid development. In this period, China Institute for Radiation Protection tissue bank mastered systematic and modern tissue banking technique by IAEA training course and gradually developed the first regional tissue bank (Shanxi Provincial Tissue Bank, SPTB) to provide tissue allograft. Benefit from training course, SPTB promoted the development of tissue transplantation by ways of training, brochure, advertisement and meeting. Tissue allograft transplantation acquired recognition from clinic and supervision and administration from government. Quality system gradually is developing and perfecting. Tissue allograft transplantation and tissue bank are developing rapidly and healthy.

  5. Development of Novel Method for Rapid Extract of Radionuclides from Solution Using Polymer Ligand Film

    Science.gov (United States)

    Rim, Jung H.

    Accurate and fast determination of the activity of radionuclides in a sample is critical for nuclear forensics and emergency response. Radioanalytical techniques are well established for radionuclides measurement, however, they are slow and labor intensive, requiring extensive radiochemical separations and purification prior to analysis. With these limitations of current methods, there is great interest for a new technique to rapidly process samples. This dissertation describes a new analyte extraction medium called Polymer Ligand Film (PLF) developed to rapidly extract radionuclides. Polymer Ligand Film is a polymer medium with ligands incorporated in its matrix that selectively and rapidly extract analytes from a solution. The main focus of the new technique is to shorten and simplify the procedure necessary to chemically isolate radionuclides for determination by alpha spectrometry or beta counting. Five different ligands were tested for plutonium extraction: bis(2-ethylhexyl) methanediphosphonic acid (H2DEH[MDP]), di(2-ethyl hexyl) phosphoric acid (HDEHP), trialkyl methylammonium chloride (Aliquat-336), 4,4'(5')-di-t-butylcyclohexano 18-crown-6 (DtBuCH18C6), and 2-ethylhexyl 2-ethylhexylphosphonic acid (HEH[EHP]). The ligands that were effective for plutonium extraction further studied for uranium extraction. The plutonium recovery by PLFs has shown dependency on nitric acid concentration and ligand to total mass ratio. H2DEH[MDP] PLFs performed best with 1:10 and 1:20 ratio PLFs. 50.44% and 47.61% of plutonium were extracted on the surface of PLFs with 1M nitric acid for 1:10 and 1:20 PLF, respectively. HDEHP PLF provided the best combination of alpha spectroscopy resolution and plutonium recovery with 1:5 PLF when used with 0.1M nitric acid. The overall analyte recovery was lower than electrodeposited samples, which typically has recovery above 80%. However, PLF is designed to be a rapid field deployable screening technique and consistency is more important

  6. Systematic Review of Breast Cancer Biology in Developing Countries (Part 2): Asian Subcontinent and South East Asia

    Energy Technology Data Exchange (ETDEWEB)

    Bhikoo, Riyaz, E-mail: riyazbhikoo@gmail.com; Srinivasa, Sanket; Yu, Tzu-Chieh [Department of Surgery, South Auckland Clinical School, University of Auckland, Auckland 1640 (New Zealand); Moss, David [Department of Surgery, Middlemore Hospital, Auckland 1640 (New Zealand); Hill, Andrew G [Department of Surgery, South Auckland Clinical School, University of Auckland, Auckland 1640 (New Zealand)

    2011-05-13

    There has been no systematic appraisal of ethnicity-based variations in breast cancer (BC) biology amongst women from developing countries. A qualitative systematic review was conducted of breast cancer size, stage, grade, histological type, extra-mammary involvement, hormone receptor status as well as patient demographics. This review includes patients from Africa, the Middle East, Eastern Europe, Mexico, the Caribbean and South America. BC in these regions present at an earlier age with large aggressive tumours. Distant metastases are frequently present at the time of diagnosis. African women have a higher frequency of triple negative tumours. Over half of Middle Eastern women have lymph node involvement at the time of diagnosis. Despite experiencing a lower incidence compared to the Ashkenazi Jewish population, Palestinian women have poorer five-year survival outcomes. The majority of women from Mexico and South America have stage two or three disease whilst over sixty percent of women from Eastern Europe have either stage one or stage two disease. The biological characteristics of BC in the Caribbean cannot be fully assessed due to a paucity of data from the region. BC amongst the developing world is characterised by an early peak age of onset with aggressive biological characteristics. Strategies that improve breast cancer awareness, address amenable risk factors and improve early detection are essential.

  7. Essential Function of Dicer in Resolving DNA Damage in the Rapidly Dividing Cells of the Developing and Malignant Cerebellum

    Directory of Open Access Journals (Sweden)

    Vijay Swahari

    2016-01-01

    Full Text Available Maintenance of genomic integrity is critical during neurodevelopment, particularly in rapidly dividing cerebellar granule neuronal precursors that experience constitutive replication-associated DNA damage. As Dicer was recently recognized to have an unexpected function in the DNA damage response, we examined whether Dicer was important for preserving genomic integrity in the developing brain. We report that deletion of Dicer in the developing mouse cerebellum resulted in the accumulation of DNA damage leading to cerebellar progenitor degeneration, which was rescued with p53 deficiency; deletion of DGCR8 also resulted in similar DNA damage and cerebellar degeneration. Dicer deficiency also resulted in DNA damage and death in other rapidly dividing cells including embryonic stem cells and the malignant cerebellar progenitors in a mouse model of medulloblastoma. Together, these results identify an essential function of Dicer in resolving the spontaneous DNA damage that occurs during the rapid proliferation of developmental progenitors and malignant cells.

  8. The positive mental health instrument: development and validation of a culturally relevant scale in a multi-ethnic asian population

    Science.gov (United States)

    2011-01-01

    Background Instruments to measure mental health and well-being are largely developed and often used within Western populations and this compromises their validity in other cultures. A previous qualitative study in Singapore demonstrated the relevance of spiritual and religious practices to mental health, a dimension currently not included in exiting multi-dimensional measures. The objective of this study was to develop a self-administered measure that covers all key and culturally appropriate domains of mental health, which can be applied to compare levels of mental health across different age, gender and ethnic groups. We present the item reduction and validation of the Positive Mental Health (PMH) instrument in a community-based adult sample in Singapore. Methods Surveys were conducted among adult (21-65 years) residents belonging to Chinese, Malay and Indian ethnicities. Exploratory and confirmatory factor analysis (EFA, CFA) were conducted and items were reduced using item response theory tests (IRT). The final version of the PMH instrument was tested for internal consistency and criterion validity. Items were tested for differential item functioning (DIF) to check if items functioned in the same way across all subgroups. Results: EFA and CFA identified six first-order factor structure (General coping, Personal growth and autonomy, Spirituality, Interpersonal skills, Emotional support, and Global affect) under one higher-order dimension of Positive Mental Health (RMSEA = 0.05, CFI = 0.96, TLI = 0.96). A 47-item self-administered multi-dimensional instrument with a six-point Likert response scale was constructed. The slope estimates and strength of the relation to the theta for all items in each six PMH subscales were high (range:1.39 to 5.69), suggesting good discrimination properties. The threshold estimates for the instrument ranged from -3.45 to 1.61 indicating that the instrument covers entire spectrums for the six dimensions. The instrument demonstrated

  9. The positive mental health instrument: development and validation of a culturally relevant scale in a multi-ethnic asian population

    Directory of Open Access Journals (Sweden)

    Vaingankar Janhavi

    2011-10-01

    Full Text Available Abstract Background Instruments to measure mental health and well-being are largely developed and often used within Western populations and this compromises their validity in other cultures. A previous qualitative study in Singapore demonstrated the relevance of spiritual and religious practices to mental health, a dimension currently not included in exiting multi-dimensional measures. The objective of this study was to develop a self-administered measure that covers all key and culturally appropriate domains of mental health, which can be applied to compare levels of mental health across different age, gender and ethnic groups. We present the item reduction and validation of the Positive Mental Health (PMH instrument in a community-based adult sample in Singapore. Methods Surveys were conducted among adult (21-65 years residents belonging to Chinese, Malay and Indian ethnicities. Exploratory and confirmatory factor analysis (EFA, CFA were conducted and items were reduced using item response theory tests (IRT. The final version of the PMH instrument was tested for internal consistency and criterion validity. Items were tested for differential item functioning (DIF to check if items functioned in the same way across all subgroups. Results: EFA and CFA identified six first-order factor structure (General coping, Personal growth and autonomy, Spirituality, Interpersonal skills, Emotional support, and Global affect under one higher-order dimension of Positive Mental Health (RMSEA = 0.05, CFI = 0.96, TLI = 0.96. A 47-item self-administered multi-dimensional instrument with a six-point Likert response scale was constructed. The slope estimates and strength of the relation to the theta for all items in each six PMH subscales were high (range:1.39 to 5.69, suggesting good discrimination properties. The threshold estimates for the instrument ranged from -3.45 to 1.61 indicating that the instrument covers entire spectrums for the six dimensions. The

  10. Development and implementation of Models of Care for musculoskeletal conditions in middle-income and low-income Asian countries.

    Science.gov (United States)

    Lim, Keith K; Chan, Madelynn; Navarra, Sandra; Haq, Syed Atiqul; Lau, Chak Sing

    2016-06-01

    This chapter discusses the challenges faced in the development and implementation of musculoskeletal (MSK) Models of Care (MoCs) in middle-income and low-income countries in Asia and outlines the components of an effective MoC for MSK conditions. Case studies of four such countries (The Philippines, Malaysia, Bangladesh and Myanmar) are presented, and their unique implementation issues are discussed. The success experienced in one high-income country (Singapore) is also described as a comparison. The Community Oriented Program for Control of Rheumatic Diseases (COPCORD) project and the role of Asia Pacific League of Associations for Rheumatology (APLAR), a professional body supporting MoC initiatives in this region, are also discussed. The experience and lessons learned from these case studies can provide useful information to guide the implementation of future MSK MoC initiatives in other middle-income and low-income countries. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Integrating Low-Cost Rapid Usability Testing into Agile System Development of Healthcare IT: A Methodological Perspective.

    Science.gov (United States)

    Kushniruk, Andre W; Borycki, Elizabeth M

    2015-01-01

    The development of more usable and effective healthcare information systems has become a critical issue. In the software industry methodologies such as agile and iterative development processes have emerged to lead to more effective and usable systems. These approaches highlight focusing on user needs and promoting iterative and flexible development practices. Evaluation and testing of iterative agile development cycles is considered an important part of the agile methodology and iterative processes for system design and re-design. However, the issue of how to effectively integrate usability testing methods into rapid and flexible agile design cycles has remained to be fully explored. In this paper we describe our application of an approach known as low-cost rapid usability testing as it has been applied within agile system development in healthcare. The advantages of the integrative approach are described, along with current methodological considerations.

  12. Living with earthquakes - development and usage of earthquake-resistant construction methods in European and Asian Antiquity

    Science.gov (United States)

    Kázmér, Miklós; Major, Balázs; Hariyadi, Agus; Pramumijoyo, Subagyo; Ditto Haryana, Yohanes

    2010-05-01

    Earthquakes are among the most horrible events of nature due to unexpected occurrence, for which no spiritual means are available for protection. The only way of preserving life and property is applying earthquake-resistant construction methods. Ancient Greek architects of public buildings applied steel clamps embedded in lead casing to hold together columns and masonry walls during frequent earthquakes in the Aegean region. Elastic steel provided strength, while plastic lead casing absorbed minor shifts of blocks without fracturing rigid stone. Romans invented concrete and built all sizes of buildings as a single, unflexible unit. Masonry surrounding and decorating concrete core of the wall did not bear load. Concrete resisted minor shaking, yielding only to forces higher than fracture limits. Roman building traditions survived the Dark Ages and 12th century Crusader castles erected in earthquake-prone Syria survive until today in reasonably good condition. Concrete and steel clamping persisted side-by-side in the Roman Empire. Concrete was used for cheap construction as compared to building of masonry. Applying lead-encased steel increased costs, and was avoided whenever possible. Columns of the various forums in Italian Pompeii mostly lack steel fittings despite situated in well-known earthquake-prone area. Whether frequent recurrence of earthquakes in the Naples region was known to inhabitants of Pompeii might be a matter of debate. Seemingly the shock of the AD 62 earthquake was not enough to apply well-known protective engineering methods throughout the reconstruction of the city before the AD 79 volcanic catastrophe. An independent engineering tradition developed on the island of Java (Indonesia). The mortar-less construction technique of 8-9th century Hindu masonry shrines around Yogyakarta would allow scattering of blocks during earthquakes. To prevent dilapidation an intricate mortise-and-tenon system was carved into adjacent faces of blocks. Only the

  13. Development of a high-speed real-time PCR system for rapid and precise nucleotide recognition

    Science.gov (United States)

    Terazono, Hideyuki; Takei, Hiroyuki; Hattori, Akihiro; Yasuda, Kenji

    2010-04-01

    Polymerase chain reaction (PCR) is a common method used to create copies of a specific target region of a DNA sequence and to produce large quantities of DNA. A few DNA molecules, which act as templates, are rapidly amplified by PCR into many billions of copies. PCR is a key technology in genome-based biological analysis, revolutionizing many life science fields such as medical diagnostics, food safety monitoring, and countermeasures against bioterrorism. Thus, many applications have been developed with the thermal cycling. For these PCR applications, one of the most important key factors is reduction in the data acquisition time. To reduce the acquisition time, it is necessary to decrease the temperature transition time between the high and low ends as much as possible. We have developed a novel rapid real-time PCR system based on rapid exchange of media maintained at different temperatures. This system consists of two thermal reservoirs and a reaction chamber for PCR observation. The temperature transition was achieved within 0.3 sec, and good thermal stability was achieved during thermal cycling with rapid exchange of circulating media. This system allows rigorous optimization of the temperatures required for each stage of the PCR processes. Resulting amplicons were confirmed by electrophoresis. Using the system, rapid DNA amplification was accomplished within 3.5 min, including initial heating and complete 50 PCR cycles. It clearly shows that the device could allow us faster temperature switching than the conventional conduction-based heating systems based on Peltier heating/cooling.

  14. Height of south Asian children in the Netherlands aged 0–20 years : secular trends and comparisons with current Asian Indian, Dutch and WHO references

    NARCIS (Netherlands)

    Wilde, J.A. de; Dommelen, P. van; Buuren, S. van; Middelkoop, B.J.C.

    2014-01-01

    Background: People from Asian populations are generally shorter than other ethnic groups. It is unknown if current universal height references are suitable for affluent South Asian children in the Netherlands. Aims: To develop height-for-age charts for contemporary South Asian children aged 0–20

  15. A New Prediction Model for Local Recurrence After Curative Rectal Cancer Surgery: Development and Validation as an Asian Collaborative Study.

    Science.gov (United States)

    Hida, Koya; Okamura, Ryosuke; Park, Soo Yeun; Nishigori, Tatsuto; Takahashi, Ryo; Kawada, Kenji; Choi, Gyu-Seog; Sakai, Yoshiharu

    2017-11-01

    Local recurrence is one of the remaining problems in rectal and rectosigmoid cancer, and it is sometimes difficult to treat. This study aimed to explore various factors that are highly related to local recurrence and to develop a new prediction model for local recurrence after curative resection. This is a retrospective cohort study SETTINGS:: This study was conducted at 2 academic hospitals in Japan and Korea. A total of 2237 patients with stage I to III rectal and rectosigmoid cancer who underwent a curative operation with a negative circumferential margin were selected. Surgical treatment was the intervention. Local recurrence was the primary outcome measure. A total of 1232 patients were selected, and rectosigmoid cancer with rare local recurrence (2/221) was excluded. A different set of 792 patients with rectal cancer were chosen for validation. Multivariate analysis showed the following factors as significant for local recurrence: poorly differentiated tumor (HR, 11.2; 95% CI, 4.5-28.0), tumor depth (HR, 5.0), lymph node metastasis (HR, 4.1), operative procedure (HR, 3.2), postoperative complications (HR, 2.9), tumor location (HR, 2.6), and CEA level (HR, 2.4); a new prediction score was created by using these factors. A poorly differentiated tumor was assigned 2 points, and all other factors were assigned 1 point each. Patients who scored more than 5 points (n = 21) were judged as "high risk," with a 2-year local recurrence rate of 66.5%. The new predictive model could also separate the patients into different risk groups in the validation set. The high-risk group had higher recurrence rates than medium- and low-risk groups (2-year local recurrence rate: 41%, 15%, and 2.1%). This study was limited by its retrospective nature and potential for selection bias. Seven factors were shown to be significantly correlated with the local recurrence of rectal cancer, and the usefulness of this new prediction model was demonstrated. See Video Abstract at http

  16. Non-linear regime shifts in Holocene Asian monsoon variability: potential impacts on cultural change and migratory patterns

    Science.gov (United States)

    Donges, J. F.; Donner, R. V.; Marwan, N.; Breitenbach, S. F. M.; Rehfeld, K.; Kurths, J.

    2015-05-01

    The Asian monsoon system is an important tipping element in Earth's climate with a large impact on human societies in the past and present. In light of the potentially severe impacts of present and future anthropogenic climate change on Asian hydrology, it is vital to understand the forcing mechanisms of past climatic regime shifts in the Asian monsoon domain. Here we use novel recurrence network analysis techniques for detecting episodes with pronounced non-linear changes in Holocene Asian monsoon dynamics recorded in speleothems from caves distributed throughout the major branches of the Asian monsoon system. A newly developed multi-proxy methodology explicitly considers dating uncertainties with the COPRA (COnstructing Proxy Records from Age models) approach and allows for detection of continental-scale regime shifts in the complexity of monsoon dynamics. Several epochs are characterised by non-linear regime shifts in Asian monsoon variability, including the periods around 8.5-7.9, 5.7-5.0, 4.1-3.7, and 3.0-2.4 ka BP. The timing of these regime shifts is consistent with known episodes of Holocene rapid climate change (RCC) and high-latitude Bond events. Additionally, we observe a previously rarely reported non-linear regime shift around 7.3 ka BP, a timing that matches the typical 1.0-1.5 ky return intervals of Bond events. A detailed review of previously suggested links between Holocene climatic changes in the Asian monsoon domain and the archaeological record indicates that, in addition to previously considered longer-term changes in mean monsoon intensity and other climatic parameters, regime shifts in monsoon complexity might have played an important role as drivers of migration, pronounced cultural changes, and the collapse of ancient human societies.

  17. A Development of Rapid, Practical and Selective Process for Preparation of Z-Oximes

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Bo Ram; Sung, Gi Hyeon; Yoon, Yongjin [Gyeongsang National Univ., Jinju (Korea, Republic of); Kim, Jeumjong [Electronic and Telecommunications Research Institute, Daejeon (Korea, Republic of)

    2013-04-15

    Oximes are important functional groups in organic chemistry due to their synthetic utility as protecting groups for carbonyl groups and their ability to form other functionalities, and their biological activity. Oximes are commonly prepared by condensing aldehydes and ketones with hydroxylamines. These reactions do not always go to completion and reaction times can be long, and therefore there has been interest in more convenient and efficient methods. To avoid the typical disadvantage, conversion of aldehydes and ketones to the corresponding oximes was accomplished by using various catalysts such as organic acid/bases, AcONa, alumina, TiO{sub 2}/SO{sub 4} silica gel, Oxone, NaOH, basic ionic liquid 1-butyl-3-methylimidazolium hydroxide, polyoxometalates, Na{sub 2}SO{sub 4}, and CuSO{sub 4}/K{sub 2}CO{sub 3} under the solvent, the solvent-free or the microwave conditions. These are one and more drowbacks such as long reaction time, use of catalysts, inconvenients due to solid-sate reaction, low yields and limitaion of some carbonyl compounds. On the other hand, H. Sharghi, et al.,14 reported the catalysis of the stereoselectivity of CuSO{sub 4} and K{sub 2}CO{sub 3} in the oximation of aldehydes and ketones under solvent-free conditions. Although this method show high selectivity, it is inconvenient for the large scale experiments and the industrial process due to the solvent-free condition. Therefore, we attempted to develop a more convenient and efficient solution method. According to the literatures, treatment of potassium carbonate with methanol generates slightly the potassium methoxide, which may be useful for forming the free NH{sub 2}OH from its salts. We describe the oximation of aldehyde and ketone using NH{sub 2}OH·HCl/K{sub 2}CO{sub 3} in methanol solvent. We selected oximation of acetophenone (1a) with hydroxylamine hydrochloride as a model and its behavior was investigated in seven solvents involving methanol (Table 1). As shown in the Entry 2 in

  18. Development of an immunochromatographic strip test for rapid detection of melamine in raw milk, milk products, and animal feed

    Science.gov (United States)

    A simple, rapid and sensitive immunogold chromatographic strip test based on a monoclonal antibody was developed for the detection of melamine (MEL) residues in raw milk, milk products and animal feed. The limit of detection was estimated to be 0.05 µg/mL in raw milk, since the detection test line ...

  19. Development of a rapid and simple Agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion annosum

    Science.gov (United States)

    Nicklas Samils; Malin Elfstrand; Daniel L. Lindner Czederpiltz; Jan Fahleson; Ake Olson; Christina Dixelius; Jan Stenlid

    2006-01-01

    Heterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at...

  20. Working Smarter Not Harder: Using a Pedagogical-Based Model in the Rapid Development of Quality Hybrid Courses

    Science.gov (United States)

    Rust, Jodi E.

    2010-01-01

    This descriptive case study explored how repurposing and a pedagogical-based instructional design model, the multimodal model (Picciano, 2009), were used to create quality courses in a rapid development setting at a Midwestern land grant university. Data triangulation was used to secure data from faculty member interviews, course syllabi, and…

  1. Rapid Facial Reactions to Emotional Facial Expressions in Typically Developing Children and Children with Autism Spectrum Disorder

    Science.gov (United States)

    Beall, Paula M.; Moody, Eric J.; McIntosh, Daniel N.; Hepburn, Susan L.; Reed, Catherine L.

    2008-01-01

    Typical adults mimic facial expressions within 1000ms, but adults with autism spectrum disorder (ASD) do not. These rapid facial reactions (RFRs) are associated with the development of social-emotional abilities. Such interpersonal matching may be caused by motor mirroring or emotional responses. Using facial electromyography (EMG), this study…

  2. Development of an ELISA for Rapid Detection of Anti-Type VII Collagen Autoantibodies in Epidermolysis Bullosa Acquisita

    National Research Council Canada - National Science Library

    Chen, M; Chan, L S; Cai, X; O'Toole, E A; Sample, J C; Woodley, D T

    1997-01-01

    .... In this study, we developed a rapid, quantitative enzyme-linked immunosorbent assay (ELISA) to detect autoantibody activity against the complete NC1 domain of type VII collagen with the use of an eukaryotic-expressed, recombinant human NC1 antigen...

  3. Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex.

    Science.gov (United States)

    Koh, Seng Fook; Tay, Sun Tee; Sermswan, Rasana; Wongratanacheewin, Surasakdi; Chua, Kek Heng; Puthucheary, Savithri D

    2012-09-01

    We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Plant Growth and Development: An Outline for a Unit Structured Around the Life Cycle of Rapid-Cycling Brassica Rapa.

    Science.gov (United States)

    Becker, Wayne M.

    This outline is intended for use in a unit of 10-12 lectures on plant growth and development at the introductory undergraduate level as part of a course on organismal biology. The series of lecture outlines is structured around the life cycle of rapid-cycling Brassica rapa (RCBr). The unit begins with three introductory lectures on general plant…

  5. Nationwide survey of nutritional management in an Asian upper-middle income developing country government hospitals: Combination of quantitative survey and focus group discussion.

    Science.gov (United States)

    Chittawatanarat, K; Tosanguan, K; Chaikledkaew, U; Tejavanija, S; Teerawattananon, Y

    2016-08-01

    The objective of this study was to identify the differences in pattern, process, and management of nutrition care in government hospitals in Thailand (an Asian upper-middle income developing country). This is a combination of a quantitative nationwide questionnaire survey and focus group discussions. A total of 2300 questionnaires were sent to government hospitals across Thailand. The responders were divided by routine-nutrition screening/assessment unit vs. non-routine-nutrition screening/assessment unit (RSA vs. NRSA). The comparison between the groups was reported as percentage and cross-sectional odds ratio (CS-OR) with 95% confidence interval (CI). The significant difference was defined as p Nutrition Triage (BNT), 21.2% Subjective Global Assessment (SGA) and 20.2% Nutrition Alert Form (NAF). The RSA was significantly higher in proportion for the role of the nurses (RSA vs. NRSA; CS-OR [95% CI]: 68.3% vs. 11.9%; 15.8 [11.1 to 22.7]; p nutrition management guidelines (60.6% vs. 2.8%; 53.6 [29.6 to 102.8]; p nutrition (91.4% vs.69.7%; 4.6 [2.9 to 7.4]; p Nutrition screening/assessment tools were found to be varied in Thailand. RSA affected the nutrition management working process and the types of nutrition support. The main barriers of RSA implementation were inconsistency of policy and reimbursement, acceptable workload, and national guidance as regards - screening/assessment tools. Copyright © 2016 European Society for Clinical Nutrition and Metabolism. Published by Elsevier Ltd. All rights reserved.

  6. Asian Citrus Psyllid Expression Profiles Suggest Candidatus Liberibacter Asiaticus-Mediated Alteration of Adult Nutrition and Metabolism, and of Nymphal Development and Immunity.

    Directory of Open Access Journals (Sweden)

    Meenal Vyas

    Full Text Available The Asian citrus psyllid (ACP Diaphorina citri Kuwayama (Hemiptera: Psyllidae is the insect vector of the fastidious bacterium Candidatus Liberibacter asiaticus (CLas, the causal agent of citrus greening disease, or Huanglongbing (HLB. The widespread invasiveness of the psyllid vector and HLB in citrus trees worldwide has underscored the need for non-traditional approaches to manage the disease. One tenable solution is through the deployment of RNA interference technology to silence protein-protein interactions essential for ACP-mediated CLas invasion and transmission. To identify psyllid interactor-bacterial effector combinations associated with psyllid-CLas interactions, cDNA libraries were constructed from CLas-infected and CLas-free ACP adults and nymphs, and analyzed for differential expression. Library assemblies comprised 24,039,255 reads and yielded 45,976 consensus contigs. They were annotated (UniProt, classified using Gene Ontology, and subjected to in silico expression analyses using the Transcriptome Computational Workbench (TCW (http://www.sohomoptera.org/ACPPoP/. Functional-biological pathway interpretations were carried out using the Kyoto Encyclopedia of Genes and Genomes databases. Differentially expressed contigs in adults and/or nymphs represented genes and/or metabolic/pathogenesis pathways involved in adhesion, biofilm formation, development-related, immunity, nutrition, stress, and virulence. Notably, contigs involved in gene silencing and transposon-related responses were documented in a psyllid for the first time. This is the first comparative transcriptomic analysis of ACP adults and nymphs infected and uninfected with CLas. The results provide key initial insights into host-parasite interactions involving CLas effectors that contribute to invasion-virulence, and to host nutritional exploitation and immune-related responses that appear to be essential for successful ACP-mediated circulative, propagative CLas

  7. Asian Citrus Psyllid Expression Profiles Suggest Candidatus Liberibacter Asiaticus-Mediated Alteration of Adult Nutrition and Metabolism, and of Nymphal Development and Immunity.

    Science.gov (United States)

    Vyas, Meenal; Fisher, Tonja W; He, Ruifeng; Nelson, William; Yin, Guohua; Cicero, Joseph M; Willer, Mark; Kim, Ryan; Kramer, Robin; May, Greg A; Crow, John A; Soderlund, Carol A; Gang, David R; Brown, Judith K

    2015-01-01

    The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is the insect vector of the fastidious bacterium Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus greening disease, or Huanglongbing (HLB). The widespread invasiveness of the psyllid vector and HLB in citrus trees worldwide has underscored the need for non-traditional approaches to manage the disease. One tenable solution is through the deployment of RNA interference technology to silence protein-protein interactions essential for ACP-mediated CLas invasion and transmission. To identify psyllid interactor-bacterial effector combinations associated with psyllid-CLas interactions, cDNA libraries were constructed from CLas-infected and CLas-free ACP adults and nymphs, and analyzed for differential expression. Library assemblies comprised 24,039,255 reads and yielded 45,976 consensus contigs. They were annotated (UniProt), classified using Gene Ontology, and subjected to in silico expression analyses using the Transcriptome Computational Workbench (TCW) (http://www.sohomoptera.org/ACPPoP/). Functional-biological pathway interpretations were carried out using the Kyoto Encyclopedia of Genes and Genomes databases. Differentially expressed contigs in adults and/or nymphs represented genes and/or metabolic/pathogenesis pathways involved in adhesion, biofilm formation, development-related, immunity, nutrition, stress, and virulence. Notably, contigs involved in gene silencing and transposon-related responses were documented in a psyllid for the first time. This is the first comparative transcriptomic analysis of ACP adults and nymphs infected and uninfected with CLas. The results provide key initial insights into host-parasite interactions involving CLas effectors that contribute to invasion-virulence, and to host nutritional exploitation and immune-related responses that appear to be essential for successful ACP-mediated circulative, propagative CLas transmission.

  8. China's forest products trade and its influencing factors: A structural analysis based on the Asian International Input-Output Table

    OpenAIRE

    加賀爪, 優; ウフル, ロシャングリ; 波多野, 佑美

    2014-01-01

    China is one of the major consumers of forest products, and its trade with developed countries has vastly expanded after it joined the WTO and abolished tariffs. The structure of China's import and export trade of forest products has also greatly changed alongside rapid economic growth. In this study, we focus on China's forest products trade with its main trading partners. By analyzing these trade patterns based on the Asian International Input-Output Table and the Japan-China International ...

  9. Spatial–Temporal Patterns and Driving Factors of Rapid Urban Land Development in Provincial China: A Case Study of Jiangsu

    Directory of Open Access Journals (Sweden)

    Qingke Yang

    2017-12-01

    Full Text Available Since its economic reform and opening-up, China has undergone unprecedented urbanization, where massive areas of rural land have been converted into urban use. Urban land development plays an important role in dynamic urban economic development. This study aims to contribute to the understanding of urban land development and its mechanisms in China. We conceptualized the mechanism of urban land development from multiple perspectives based on a case study in Jiangsu Province. We employed the methods of global and local spatial auto-correlation detection and spatial lag model to analyze the provincial land use conveyance and survey data from 2004 to 2008 and 2009 to 2012 to understand the dynamics of urban land development. The results show that urban land development varies significantly across different time periods, spatial scales, and regions in Jiangsu. Higher absolute urban land development mainly occurred in Sunan, expanding to Subei and Suzhong, while faster development occurred mainly in Subei, where the initial bases were lower but had strong economic growth potential. The regression analysis shows that market activities and administrative levels had played a more critical role in driving urban land development, which suggests that rapid urban land development has institutional and market foundations. Urban land development was not only a consequence of economic development but also a strategy of local governments to stimulate and govern the urban economy. This study enriches the literature on urban dynamics by providing an institutional understanding of rapid urban land development in a transitional economy.

  10. A prototype Lab Box with DSK 'C6711/13 for rapid DSP algorithm development

    OpenAIRE

    Bertini, Graziano

    2007-01-01

    A system based on the TMS320C6711 DSK Starter Kit, enclosed in a special chassis, designed for real-time DSP algorithms testing, is described. Some evaluation of MATLab software Rapid Prototyping tools for a "Virtual Dubbing" application on damaged speech is also given.

  11. Rapid Naming Speed Components and Reading Development in a Consistent Orthography

    Science.gov (United States)

    Georgiou, George K.; Papadopoulos, Timothy C.; Fella, Argyro; Parrila, Rauno

    2012-01-01

    We examined how rapid automatized naming (RAN) components--articulation time and pause time--predict word and text reading fluency in a consistent orthography (Greek). In total, 68 children were followed from Grade 2 to Grade 6 and were assessed three times on RAN (Digits and Objects), phonological awareness, orthographic processing, speed of…

  12. Governing Rapid Growth in Asia: State-led Development in Historical Perspective

    NARCIS (Netherlands)

    T-W. Ngo (Tak-Wing)

    2009-01-01

    textabstractRapid growth in Asia has often been explained in terms of effective policies pursued by a “developmental state”. In particular, countries in East Asia are said to be characterized by the presence of a strong state with technocratic capacity and social embeddedness. This inaugural address

  13. Development of a novel multiplex PCR assay for rapid detection of virulence associated genes of Pasteurella multocida from pigs.

    Science.gov (United States)

    Rajkhowa, S

    2015-09-01

    As the pathogenicity of Pasteurella multocida is associated with various virulence factors (VFs), the aim of the study was to develop a novel multiplex PCR (m-PCR) assay for the rapid detection of important virulence associated genes (VAGs) of P. multocida isolates from pigs. The target recognized VFs used in the study were diverse adhesins (ptfA and pfhA), toxins (toxA), siderophores (tonB and hgbA), sialidases (nanB, nanH) and outer membrane proteins (ompA, ompH, oma87 and plpB). The primers for the genes encoding these VFs were designed by primer3 software (http://bioinfo.ut.ee/primer3-0.4.0/) using gene sequences available in Genbank. The detection limit of the developed assay was 10(2)  CFU ml(-1) . The m-PCR did not produce any nonspecific amplification products when tested against Bordetella bronchiseptica which also commonly infects pigs. We applied m-PCR to the field samples, and the results obtained were the same as the single PCR results. The developed assay would be very useful for veterinary diagnostic laboratories and for others interested in the rapid virulence profiling of porcine P. multocida isolates circulating in the piggeries. The study reports the development and evaluation of a novel multiplex PCR assay for the rapid detection of 11 important VAGs of Pasteurella multocida isolates from pigs. Rapid and simultaneous detection of recognized VFs of the organism are essential to know the virulo-types of P. multocida isolates circulating in the piggeries. The developed novel assay will be very useful for the rapid detection of VAGs of P. multocida isolates from pigs. © 2015 The Society for Applied Microbiology.

  14. South Asian co-operation in population programmes.

    Science.gov (United States)

    Iqbal, M A

    1989-12-01

    Efforts by governments of South Asian countries to improve the economic growth and welfare of the people are being negated by high fertility. In some countries, the expenditures on health programs have increased and the programs have expanded rapidly without any concomitant improvement in efficiency or cost-effectiveness. This highlights the urgency of tackling the problems of not only population growth but also of managing population programs. There are similarities as well as differences in the management of population programs in the South Asian countries. Bangladesh has a population policy with a strong political will behind it. It also has a sound administrative structure and organization. Some areas that need attention are building up the operational relationship between health and family planning personnel, improved infrastructure development, more community involvement, and better evaluation. Some states in India have shown outstanding success in population programs, and some have successfully experimented with beyond family planning methods. A greater awareness of family planning among health staff, a solid information base for decision making, and management training for medical officers and staff are also needed. Pakistan's population program has had uneven results in the past, but it has now developed multi-sectoral components in decentralized field activities, involvement of local organizations and leaders, and better information, education, and social marketing, but it still needs to plan for evaluation and strengthen management training. Of the South Asian countries, Sri Lanka, with a high level of literacy and health care, has shown the best achievement in its social sector. The coverage through the maternal and child health service delivery system has been commendable with 94% of deliveries through institutions. Regional meetings have identified program management problem areas in 1) planning, control, and coordination of program implementation, 2

  15. Mental Health and Asian Americans

    Science.gov (United States)

    ... Data > Minority Population Profiles > Asian American > Mental Health Mental Health and Asian Americans Suicide was the 9th leading ... Americans is half that of the White population. MENTAL HEALTH STATUS Serious psychological distress among adults 18 years ...

  16. Development a rapid and accurate multiplex real time PCR method for the detection Chlamydia trachomatis and Mycoplasma hominis.

    Science.gov (United States)

    Safarkar, Roya; Mehrabadi, Jalil Fallah; Noormohammadi, Zahra; Mirnejad, Reza

    2017-11-01

    Sexually transmitted diseases easily spread among sexually active people and often have no symptoms. Rapid and accurate method for detecting these infections are necessary in early stages. The traditional detection methods of them are difficult and time-consuming. In this study, multiplex real time PCR was optimized for rapid identification of Chlamydia trachomatis and Mycoplasma hominis in a single tube and was performed with our designed primers. The sensitivity test was carried out to designed primers with diluted genomic DNA. To defined the specificity, non STD bacteria were used as DNA template. This study indicated that the developed multiplex real time PCR can be an effective alternative procedure to the conventional methods for rapid and accurate identification of C Chlamydia trachomatis and Mycoplasma hominis. Multiplex real-time PCR Results of them were checked with melting curves. The sensitivity of our designed primer by multiplex real time PCR for Chlamydia trachomatis and Mycoplasma hominis were 4.78×1010 and 8.35×1010 , respectively, Which the primers did not amplify any product from a non-STD species. Multiplex real time PCR by our new primers and analysis of melting curves were successfully usable for rapid and accurate detection of Chlamydia trachomatis and Mycoplasma hominis. This assay instead of traditional culture method, has considerable potential to be rapid, accurate and highly sensitive molecular diagnostic tool for simultaneous and direct detection. © 2017 Wiley Periodicals, Inc.

  17. Development of a loop-mediated isothermal amplification assay for rapid detection of Burkholderia mallei.

    Science.gov (United States)

    Mirzai, S; Safi, S; Mossavari, N; Afshar, D; Bolourchian, M

    2016-08-31

    The present study was conducted to establish a Loop-mediated isothermal amplification (LAMP) technique for the rapid detection of B. mallei the etiologic agent of glanders, a highly contagious disease of equines. A set of six specific primers targeting integrase gene cluster were designed for the LAMP test. The reaction was optimized using different temperatures and time intervals. The specificity of the assay was evaluated using DNA from B.pseudomallei and Pseudomonas aeruginosa. The LAMP products were analyzed both visually and under UV light after electrophoresis. The optimized conditions were found to be at 63ºC for 60 min. The assay showed high specificity and sensitivity. It was concluded that the established LAMP assay is a rapid, sensitive and practical tool for detection of B. mallei and early diagnosis of glanders.

  18. Development of Rapid Temporal Processing and Its Impact on Literacy Skills in Primary School Children

    Science.gov (United States)

    Steinbrink, Claudia; Zimmer, Karin; Lachmann, Thomas; Dirichs, Martin; Kammer, Thomas

    2014-01-01

    In a longitudinal study, auditory and visual temporal order thresholds (TOTs) were investigated in primary school children (N = 236; mean age at first data point = 6;7) at the beginning of Grade 1 and the end of Grade 2 to test whether rapid temporal processing abilities predict reading and spelling at the end of Grades 1 and 2. Auditory and…

  19. Development of lidocaine-coated microneedle product for rapid, safe, and prolonged local analgesic action.

    Science.gov (United States)

    Zhang, Ying; Brown, Ken; Siebenaler, Kris; Determan, Amy; Dohmeier, Daniel; Hansen, Kris

    2012-01-01

    To demonstrate rapid (~1 min) lidocaine delivery using 3M's solid microstructured transdermal system (sMTS) for prolonged, local analgesic action. Polymeric microneedles were fabricated via injection molding and then dip-coated using an aqueous lidocaine formulation. The amount of lidocaine coated onto the microneedles was determined by high performance liquid chromatography (HPLC). To assess drug delivery and dermal pharmacokinetics, lidocaine-coated microneedles were inserted into domestic swine. Skin punch biopsies were collected and analyzed to determine lidocaine concentration in skin using HPLC-mass spectrometry (LC-MS). Commercial lidocaine/prilocaine EMLA (Eutectic Mixture of Local Anesthetic) cream was used as comparative control. Lidocaine dissolves rapidly off the microneedles and into skin such that the 1-min wear time achieves or exceeds lidocaine tissue levels needed to cause analgesia. This therapeutic threshold (100 ng/mg) was estimated by measuring the total amount of lidocaine and prilocaine in skin following a 1 h EMLA application. When co-formulated with 0.03 wt% vasoconstrictor-epinephrine, the concentration of lidocaine in tissue was maintained above 100 ng/mg for approximately 90 min. 3M's sMTS can be used to provide rapid delivery of lidocaine for local analgesia up to 90 min.

  20. The Development and Validation of a Rapid Assessment Tool of Primary Care in China

    Science.gov (United States)

    Mei, Jie; Liang, Yuan; Shi, LeiYu; Zhao, JingGe; Wang, YuTan; Kuang, Li

    2016-01-01

    Introduction. With Chinese health care reform increasingly emphasizing the importance of primary care, the need for a tool to evaluate primary care performance and service delivery is clear. This study presents a methodology for a rapid assessment of primary care organizations and service delivery in China. Methods. The study translated and adapted the Primary Care Assessment Tool-Adult Edition (PCAT-AE) into a Chinese version to measure core dimensions of primary care, namely, first contact, continuity, comprehensiveness, and coordination. A cross-sectional survey was conducted to assess the validity and reliability of the Chinese Rapid Primary Care Assessment Tool (CR-PCAT). Eight community health centers in Guangdong province have been selected to participate in the survey. Results. A total of 1465 effective samples were included for data analysis. Eight items were eliminated following principal component analysis and reliability testing. The principal component analysis extracted five multiple-item scales (first contact utilization, first contact accessibility, ongoing care, comprehensiveness, and coordination). The tests of scaling assumptions were basically met. Conclusion. The standard psychometric evaluation indicates that the scales have achieved relatively good reliability and validity. The CR-PCAT provides a rapid and reliable measure of four core dimensions of primary care, which could be applied in various scenarios. PMID:26885509

  1. Scrolling and Strolling, Asian Style

    Science.gov (United States)

    Sterling, Joan

    2012-01-01

    In this article, the author describes a lesson on Asian cultures. Asian cultures demonstrate respect for nature through their art. Students learned how to use Asian brush techniques and designs to create scrolls. They also learned how to write Haiku, a three-line form of poetry that uses a pattern of syllables.

  2. Effect of brief daily resistance training on rapid force development in painful neck and shoulder muscles: randomized controlled trial.

    Science.gov (United States)

    Jay, Kenneth; Schraefel, Mc; Andersen, Christoffer H; Ebbesen, Frederik S; Christiansen, David H; Skotte, Jørgen; Zebis, Mette K; Andersen, Lars L

    2013-09-01

    To determine the effect of small daily amounts of progressive resistance training on rapid force development of painful neck/shoulder muscles. 198 generally healthy adults with frequent neck/shoulder muscle pain (mean: age 43.1 years, computer use 93% of work time, 88% women, duration of pain 186 day during the previous year) were randomly allocated to 2- or 12 min of daily progressive resistance training with elastic tubing or to a control group receiving weekly information on general health. A blinded assessor took measures at baseline and at 10-week follow-up; participants performed maximal voluntary contractions at a static 90-degree shoulder joint angle. Rapid force development was determined as the rate of torque development and maximal muscle strength was determined as the peak torque. Compared with the control group, rate of torque development increased 31.0 Nm s(-1) [95% confidence interval: (1.33-11.80)] in the 2-min group and 33.2 Nm s(-1) (1.66-12.33) in the 12-min group from baseline to 10-week follow-up, corresponding to an increase of 16.0% and 18.2% for the two groups, respectively. The increase was significantly different compared to controls (Pmuscle strength increased only ~5-6% [mean and 95% confidence interval for 2- and 12-min groups to control, respectively: 2.5 Nm (0.05-0.73) and 2.2 Nm (0.01-0.70)]. No significant differences between the 2- and 12-min groups were evident. A weak but significant relationship existed between changes in rapid force development and pain (r = 0.27, Pmuscle strength and pain. Small daily amounts of progressive resistance training in adults with frequent neck/shoulder pain increases rapid force development and, to a less extent, maximal force capacity. © 2013 The Authors Clinical Physiology and Functional Imaging © 2013 Scandinavian Society of Clinical Physiology and Nuclear Medicine.

  3. Comparative Studies on Vehicle Related Policies for Air Pollution Reduction in Ten Asian Countries

    Directory of Open Access Journals (Sweden)

    Keiko Hirota

    2010-01-01

    Full Text Available Asian countries are facing major air pollution problems due to rapid economic growth, urbanization and motorization. Mortality and respiratory diseases caused by air pollution are believed to be endemic in major cities of these countries. Regulations and standards are the first requirement for reducing emissions from both fixed and mobile sources. This paper emphasizes monitoring problems such as vehicle registration systems, inspection and maintenance (I/M systems and fuel quality monitoring systems for vehicles in use. Monitoring problems in developing countries share similar characteristics such as a weakness in government initiatives and inadequate operation of government agencies, which results from a lack of human resources and availability of adequate facilities. Finally, this paper proposes a method to assure air quality improvements under the different shares of emission regulations in these Asian countries and introduces an example of an evaluation method based on a policy survey to improve air quality.

  4. Asian American Health

    Science.gov (United States)

    Every racial or ethnic group has specific health concerns. Differences in the health of groups can result from Genetics Environmental factors Access to care Cultural factors On this page, you'll find links to health issues that affect Asian Americans.

  5. Asian fungal fermented food

    NARCIS (Netherlands)

    Nout, M.J.R.; Aidoo, K.E.

    2010-01-01

    In Asian countries, there is a long history of fermentation of foods and beverages. Diverse micro-organisms, including bacteria, yeasts and moulds, are used as starters, and a wide range of ingredients can be made into fermented foods. The main raw materials include cereals, leguminous seeds,

  6. HIV Among Asians

    Science.gov (United States)

    ... Prevention VIH En Español Get Tested Find an HIV testing site near you. Enter ZIP code or city Follow HIV/AIDS CDC HIV CDC HIV/AIDS See RSS | ... Email Updates on HIV Syndicated Content Website Feedback HIV Among Asians in the United States Format: Select ...

  7. Malaysian Cinema, Asian Film

    NARCIS (Netherlands)

    Heide, van der William

    2002-01-01

    This title series departs from traditional studies of national cinema by accentuating the intercultural and intertextual links between Malaysian films and Asian (as well as European and American) film practices. Using cross-cultural analysis, the author characterizes Malaysia as a pluralist society

  8. Development of an OP9 derived cell line as a robust model to rapidly study adipocyte differentiation.

    Directory of Open Access Journals (Sweden)

    Jacqueline M Lane

    Full Text Available One hallmark of obesity is adipocyte hypertrophy and hyperplasia. To gain novel insights into adipose biology and therapeutics, there is a pressing need for a robust, rapid, and informative cell model of adipocyte differentiation for potential RNAi and drug screens. Current models are prohibitive for drug and RNAi screens due to a slow differentiation time course and resistance to transfection. We asked if we could create a rapid, robust model of adipogenesis to potentially enable rapid functional and obesity therapeutic screens. We generated the clonal population OP9-K, which differentiates rapidly and reproducibly, and displays classic adipocyte morphology: rounded cell shape, lipid accumulation, and coalescence of lipids into a large droplet. We further validate the OP9-K cells as an adipocyte model system by microarray analysis of the differentiating transcriptome. OP9-K differentiates via known adipogenic pathways, involving the transcriptional activation and repression of common adipose markers Plin1, Gata2, C/Ebpα and C/Ebpβ and biological pathways, such as lipid metabolism, PPARγ signaling, and osteogenesis. We implemented a method to quantify lipid accumulation using automated microscopy and tested the ability of our model to detect alterations in lipid accumulation by reducing levels of the known master adipogenic regulator Pparγ. We further utilized our model to query the effects of a novel obesity therapeutic target, the transcription factor SPI1. We determine that reduction in levels of Spi1 leads to an increase in lipid accumulation. We demonstrate rapid, robust differentiation and efficient transfectability of the OP9-K cell model of adipogenesis. Together with our microscopy based lipid accumulation assay, adipogenesis assays can be achieved in just four days' time. The results of this study can contribute to the development of rapid screens with the potential to deepen our understanding of adipose biology and efficiently

  9. Development of a simultaneous lateral flow strip test for the rapid and simple detection of deoxynivalenol and zearalenone.

    Science.gov (United States)

    Kim, Kyeong-Yeol; Shim, Won-Bo; Kim, Jeong-Sook; Chung, Duck-Hwa

    2014-10-01

    The objective of this study was to develop a 1-step simultaneous lateral flow strip test for the rapid and simple detection of deoxynivalenol (DON) and zearalenone (ZEA) in grains. Two monoclonal antibodies (MAbs) against DON and ZEA were respectively conjugated with gold nanoparticles and used to develop a lateral flow strip test for a single toxin and multiple toxins. First, individual lateral flow strips for a single toxin were optimized, and their conditions were used to develop a simultaneous lateral flow strip for multiple toxins. Limits of detection of both lateral flow strip tests for DON and ZEA were the same (DON: 50 ng/mL, ZEA: 1 ng/mL). Both methods showed cross-reactivity for α-zearalenol and β-zearalenol, but no cross-reaction to other mycotoxins. The results can be completed obtained within 15 min. The cut-off values of the simultaneous lateral flow strip for the spiked rice and corn were 500 and 10 ng/g for DON and ZEA, respectively. The results demonstrated that the developed simultaneous lateral flow strip test offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site detection of DON and ZEA in food and agricultural commodities. Simultaneous lateral strip test is useful for a rapid detection of DON and ZEA at a time in food and grain samples. © 2014 Institute of Food Technologists®

  10. Sexuality and human rights: an Asian perspective.

    Science.gov (United States)

    Laurent, Erick

    2005-01-01

    In Asia, the lesbian and gay rights movements are clearly dominated by activists, who tend to think in terms of a binary opposition (homo- vs hetero-) and clear-cut categories. Based on "Western patterns," the approach is practical, the arguments based on minority rights. "Coming out" is often perceived as a "white model" bringing more problems than real freedom. On the contrary, "Asian values" put the emphasis on family and social harmony, often in contradiction to what is pictured as "lesbian and gay rights." Homophobia follows very subtle ways in Asian countries. Asian gays have to negotiate their freedom, lifestyle and identities in an atmosphere of heterosexism, and not the endemic violent homophobia prevalent in many western countries. In Asia, one's identity relates to one's position in the group and sexuality plays a relatively insignificant role in its cultural construction. That Asian gays often marry and have children shows the elasticity their sexual identity encompasses. Fluidity of sexuality does not really match the Western approach in terms of essentialist categories that have a right to exist. Most Asian societies can be thought of as "tolerant" as long as homosexuality remains invisible. Procreative sexuality can be seen as a social duty, and heterosexual marriage is often not considered incompatible with a "homosexual life." The development of the Internet has even facilitated the encounters while allowing secrecy. Unfortunately, the traditional figures of transgender and transvestites have often been separated from the gay liberation movement.

  11. Consensus on Changing Trends, Attitudes, and Concepts of Asian Beauty.

    Science.gov (United States)

    Liew, Steven; Wu, Woffles T L; Chan, Henry H; Ho, Wilson W S; Kim, Hee-Jin; Goodman, Greg J; Peng, Peter H L; Rogers, John D

    2016-04-01

    Asians increasingly seek non-surgical facial esthetic treatments, especially at younger ages. Published recommendations and clinical evidence mostly reference Western populations, but Asians differ from them in terms of attitudes to beauty, structural facial anatomy, and signs and rates of aging. A thorough knowledge of the key esthetic concerns and requirements for the Asian face is required to strategize appropriate facial esthetic treatments with botulinum toxin and hyaluronic acid (HA) fillers. The Asian Facial Aesthetics Expert Consensus Group met to develop consensus statements on concepts of facial beauty, key esthetic concerns, facial anatomy, and aging in Southeastern and Eastern Asians, as a prelude to developing consensus opinions on the cosmetic facial use of botulinum toxin and HA fillers in these populations. Beautiful and esthetically attractive people of all races share similarities in appearance while retaining distinct ethnic features. Asians between the third and sixth decades age well compared with age-matched Caucasians. Younger Asians' increasing requests for injectable treatments to improve facial shape and three-dimensionality often reflect a desire to correct underlying facial structural deficiencies or weaknesses that detract from ideals of facial beauty. Facial esthetic treatments in Asians are not aimed at Westernization, but rather the optimization of intrinsic Asian ethnic features, or correction of specific underlying structural features that are perceived as deficiencies. Thus, overall facial attractiveness is enhanced while retaining esthetic characteristics of Asian ethnicity. Because Asian patients age differently than Western patients, different management and treatment planning strategies are utilized. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to Table of Contents or the online Instructions to Authors www

  12. Development of a Fieldable, Rapid, Accurate and Sensitive Bio-Electronic, DNA Biosensor

    Science.gov (United States)

    2004-12-01

    single molecule of DNA or RNA and therefore does not require the use of PCR. The sensor chip can be engineered with an array of hundreds of independent test sites, which allows for confirmatory tests leading to a high level of specificity, the ability to screen for multiple threat agents simultaneously, and the capability to detect genetically engineered organisms. Biological agents continue to pose a major threat to U.S. troops as well as U.S. civilians both domestically and overseas. Rapid, accurate and sensitive detection and identification of biological agents is

  13. Rapid development of a mycotic aneurysm of the intracranial artery secondary to Scedosporium apiospermum sinusitis

    Directory of Open Access Journals (Sweden)

    Yoshihiko Ogawa

    2016-12-01

    Full Text Available An 85-year-old man complained of a 2-month history of pain on the left side of his face. Brain computed tomography (CT and magnetic resonance imaging/magnetic resonance angiography did not clearly show any intracranial abnormality and only showed fluid effusion in his left sphenoid sinus. Filamentous fungi were detected from the left sphenoid sinus specimen. The isolate was Scedosporium apiospermum. He was empirically treated with voriconazole, to which the isolate was susceptible. His consciousness decreased rapidly. Urgent 3D-CT angiography revealed an intracranial aneurysm near the left sphenoid sinus. Despite urgent coil embolization, the aneurysm ruptured, and he died.

  14. Development of a SERS-Based Rapid Vertical Flow Assay for Point-of-Care Diagnostics.

    Science.gov (United States)

    Clarke, O J R; Goodall, B L; Hui, H P; Vats, N; Brosseau, C L

    2017-02-07

    Point-of-care (POC) diagnostic testing platforms are a growing sector of the healthcare industry as they offer the advantages of rapid provision of results, ease of use, reduced cost, and the ability to link patients to care. While many POC tests are based on chromatographic flow assay technology, this technology suffers from a lack of sensitivity along with limited capacity for multiplexing and quantitative analysis. Several recent reports have begun to investigate the feasibility of coupling chromatographic flow platforms to more advanced read-out technologies which in turn enable on-site acquisition, storage, and transmission of important healthcare metrics. One such technology being explored is surface-enhanced Raman spectroscopy or SERS. In this work, SERS is coupled for the first time to a rapid vertical flow (RVF) immunotechnology for detection of anti-HCV antibodies in an effort to extend the capabilities of this commercially available diagnostic platform. High-quality and reproducible SERS spectra were obtained using reporter-modified gold nanoparticles (AuNPs). Serial dilution studies indicate that the coupling of SERS with RVF technology shows enormous potential for next-generation POC diagnostics.

  15. Asian monsoon extremes and humanity's response over the past millennium

    Science.gov (United States)

    Buckley, B. M.; Lieberman, V. B.; Zottoli, B.

    2012-12-01

    The first decade of the 21st century has seen significant development in the production of paleo proxies for the Asian monsoon, exemplified by the Monsoon Asian Drought Atlas that was comprised of more than 300 tree ring chronologies. Noteworthy among them is the Vietnamese cypress tree-ring record which reveals that the two worst droughts of the past 7 centuries, each more than a decade in length, coincided with the demise of the Khmer civilization at Angkor in the early 15th century CE. The 18th century was nearly as tumultuous a period across Southeast Asia, where several polities fell against a backdrop of epic decadal-scale droughts. At this time all of the region's charter states saw rapid realignment in the face of drought, famine, disease and a raft of related and unrelated social issues. Several other droughts, some more extreme but of lesser duration, punctuate the past millennium, but appear to have had little societal impact. Historical documentation is being used not only to provide corroborative evidence of tree-ring reconstructed climate extremes, but to attempt to understand the dynamics of the coupled human-natural systems involved, and to define what kinds of thresholds need to be reached before societies respond. This paleo perspective can assist our analyses of the role of climate extremes in the collapse or disruption of regional societies, a subject of increasing concern given the uncertainties surrounding projections for future climate across the highly populated areas of Asia.

  16. BIBLE A whole-air sampling as a window on Asian biogeochemistry

    Science.gov (United States)

    Elliott, Scott; Blake, Donald R.; Blake, Nicola J.; Dubey, Manvendra K.; Rowland, F. Sherwood; Sive, Barkley C.; Smith, Felisa A.

    2003-02-01

    Asian trace gas and aerosol emissions into carbon, nitrogen, and other elemental cycles will figure prominently in near term Earth system evolution. Atmospheric hydrocarbon measurements resolve numerous chemical species and can be used to investigate sourcing for key geocarriers. A recent aircraft study of biomass burning and lightning (BIBLE A) explored the East Asian atmosphere and was unique in centering on the Indonesian archipelago. Samples of volatile organics taken over/between the islands of Japan, Saipan, Java, and Borneo are here examined as a guide to whole-air-based studies of future Asian biogeochemistry. The midlatitude onshore/offshore pulse and tropical convection strongly influence concentration distributions. As species of increasing molecular weight are considered, rural, combustion, and industrial source regimes emerge. Methane-rich inputs such as waste treatment and rice cultivation are evidenced in the geostrophic outflow. The Indonesian atmosphere is rich in biomass burning markers and also those of vehicular activity. Complexity of air chemistry in the archipelago is a direct reflection of diverse topography, land use, and local economies in a rapidly developing nation. Conspicuous in its absence is the fingerprint for liquefied petroleum gas leakage, but it can be expected to appear as demand for clean fossil fuels rises along with per capita incomes. Combustion tracers indicate high nitrogen mobilization rates, linking regional terrestrial geocycles with open marine ecosystems. Sea to air fluxes are superimposed on continental and marine backgrounds for the methyl halides. However, ocean hot spots are not coordinated and suggest an intricate subsurface kinetics. Levels of long-lived anthropogenic halocarbons attest to the success of international environmental treaties while reactive chlorine containing species track industrial air masses. The dozens of hydrocarbons resolvable by gas chromatographic methods will enable monitoring of

  17. South Asian diets and insulin resistance.

    Science.gov (United States)

    Misra, Anoop; Khurana, Lokesh; Isharwal, Sumit; Bhardwaj, Swati

    2009-02-01

    A role of dietary nutrients in relation to insulin resistance has been suggested but conclusive evidence in human beings is lacking. Asian Indians and South Asians are prone to develop insulin resistance and the metabolic syndrome. In the present paper, data pertaining to nutrient intake, insulin resistance and cardiovascular risk factors in Asian Indians and South Asians have been reviewed. In these populations, several dietary imbalances have been reported: low intake of MUFA, n-3 PUFA and fibre, and high intake of fats, saturated fats, carbohydrates and trans-fatty acids (mostly related to the widespread use of Vanaspati, a hydrogenated oil). Some data suggest that these nutrient imbalances are associated with insulin resistance, dyslipidaemia and subclinical inflammation in South Asians. Specifically, in children and young individuals, a high intake of n-6 PUFA is correlated with fasting hyperinsulinaemia, and in adults, high-carbohydrate meal consumption was reported to cause hyperinsulinaemia, postprandial hyperglycaemia and hypertriacylglycerolaemia. Dietary supplementation with n-3 PUFA leads to an improved lipid profile but not insulin sensitivity. Inadequate maternal nutrition in pregnancy, low birth weight and childhood 'catch-up' obesity may be important for the development of the metabolic syndrome and diabetes. Even in rural populations, who usually consume traditional frugal diets, there is an increasing prevalence of cardiovascular risk factors and the metabolic syndrome due to changes in diets and lifestyle. Nationwide community intervention programmes aimed at creating awareness about the consequences of unhealthy food choices and replacing them by healthy food choices are urgently needed in urban and rural populations in India, other countries in South Asia and in migrant South Asians.

  18. Heat Treatment Development for a Rapidly Solidified Heat Resistant Cast Al-Si Alloy

    Science.gov (United States)

    Kasprzak, W.; Chen, D. L.; Shaha, S. K.

    2013-07-01

    Existing heat treatment standards do not properly define tempers for thin-walled castings that solidified with high solidification rates. Recently emerged casting processes such as vacuum high pressure die casting should not require long solution treatment times due to the fine microstructures arising from rapid solidification rates. The heat treatment studies involving rapidly solidified samples with secondary dendrite arm spacing between 10 and 35 μm were conducted for solution times between 30 min and 9 h and temperatures of 510 and 525 °C and for various aging parameters. The metallurgical analysis revealed that an increase in microstructure refinement could enable a reduction of solution time up to 88%. Solution treatment resulted in the dissolution of Al2Cu and Al5Mg8Si6Cu2, while Fe- and TiZrV-based phases remained partially in the microstructure. The highest strength of approximately 351 ± 9.7 and 309 ± 3.4 MPa for the UTS and YS, respectively, was achieved for a 2-step solution treatment at 510 and 525 °C in the T6 peak aging conditions, i.e., 150 °C for 100 h. The T6 temper did not yield dimensionally stable microstructure since exceeding 250 °C during in-service operation could result in phase transformation corresponding to the over-aging reaction. The microstructure refinement had a statistically stronger effect on the alloy strength than the increase in solutionizing time. Additionally, thermal analysis and dilatometer results were presented to assess the dissolution of phases during solution treatment, aging kinetics as well as dimensional stability.

  19. Murine Neonates Infected with Yersinia enterocolitica Develop Rapid and Robust Proinflammatory Responses in Intestinal Lymphoid Tissues

    Science.gov (United States)

    Siefker, David T.; Echeverry, Andrea; Brambilla, Roberta; Fukata, Masayuki; Schesser, Kurt

    2014-01-01

    Neonatal animals are generally very susceptible to infection with bacterial pathogens. However, we recently reported that neonatal mice are highly resistant to orogastric infection with Yersinia enterocolitica. Here, we show that proinflammatory responses greatly exceeding those in adults arise very rapidly in the mesenteric lymph nodes (MLN) of neonates. High-level induction of proinflammatory gene expression occurred in the neonatal MLN as early as 18 h postinfection. Marked innate phagocyte recruitment was subsequently detected at 24 h postinfection. Enzyme-linked immunosorbent spot assay (ELISPOT) analyses indicated that enhanced inflammation in neonatal MLN is contributed to, in part, by an increased frequency of proinflammatory cytokine-secreting cells. Moreover, both CD11b+ and CD11b− cell populations appeared to play a role in proinflammatory gene expression. The level of inflammation in neonatal MLN was also dependent on key bacterial components. Y. enterocolitica lacking the virulence plasmid failed to induce innate phagocyte recruitment. In contrast, tumor necrosis factor alpha (TNF-α) protein expression and neutrophil recruitment were strikingly higher in neonatal MLN after infection with a yopP-deficient strain than with wild-type Y. enterocolitica, whereas only modest increases occurred in adults. This hyperinflammatory response was associated with greater colonization of the spleen and higher mortality in neonates, while there was no difference in mortality among adults. This model highlights the dynamic levels of inflammation in the intestinal lymphoid tissues and reveals the protective (wild-type strain) versus harmful (yopP-deficient strain) consequences of inflammation in neonates. Moreover, these results reveal that the neonatal intestinal lymphoid tissues have great potential to rapidly mobilize innate components in response to infection with bacterial enteropathogens. PMID:24478090

  20. Global Change and East Asian Policy Initiatives

    OpenAIRE

    Shahid, Yusuf; Altaf, M. Anjum; Nabeshima, Kaoru

    2004-01-01

    Many East Asian economies have grown briskly in the past few years. However, future development will depend on the quality and timeliness of regional and national policy actions. The policy agenda must address the problems that buffeted the region in the late 1990s-associated with the weakness of domestic institutions and policies in the context of globalization. These problems include fin...

  1. Merging ORS Standards to Facilitate Rapid Development of Reusable Spacecraft Software Project

    Data.gov (United States)

    National Aeronautics and Space Administration — DNet has been actively pursuing strategies for shortening the software development portion of the satellite development life-cycle for some time. We recognized upon...

  2. Development of a lateral flow immunoassay strip for rapid detection of CagA antigen of Helicobacter pylori.

    Science.gov (United States)

    Karakus, Cebrail

    2015-01-01

    About half of the world populations are known to be infected with Helicobacter pylori. The CagA antigen secreting strains provoke severe mucosal damages and act as a risk factor for the development of peptic ulceration and gastric cancer. A lateral flow immunoassay (LFIA) strip was developed based on sandwich format for rapid detection of CagA antigen of H. pylori using gold conjugated monoclonal antibody. This LFIA strip will provide a good aid in the diagnosis of CagA-secreting H. pylori within 10 min instead of time consuming, expensive and laborious invasive approaches.

  3. Involving South Asian patients in clinical trials.

    Science.gov (United States)

    Hussain-Gambles, M; Leese, B; Atkin, K; Brown, J; Mason, S; Tovey, P

    2004-10-01

    . Important decisions, such as participation in clinical trials, are likely to be made by those family members who are fluent in English and younger. Social class appears to be more important than ethnicity, and older South Asian people and those from working class backgrounds appear to be more mistrustful. Approachable patients (of the same gender, social class and fluent in English) tend to be 'cherry picked' to clinical trials. This practice was justified because of a lack of time and resources and inadequate support. South Asian patients might be systematically excluded from trials owing to the increased cost and time associated with their inclusion, particularly in relation to the language barrier. Under-representation might also be due to passive exclusion associated with cultural stereotypes. Other characteristics such as gender, age, educational level and social class can also affect trial inclusion. Effective strategies for South Asian recruitment to clinical trials include: using multi-recruitment strategies; defining the demographic and social profiles of the population to be included; using focus groups to identify any potential barriers; consulting representative community members to provide assistance in the study; ensuring eligibility criteria are set as wide as possible; developing educational and recruitment approaches to attract ethnic minority health professionals; ensuring health professionals are adequately trained in culturally and ethnically orientated service provision; determining the most effective mass media to use in study promotion and recruitment; and targeting inner-city, single-handed practices likely to have high ethnic minority populations. Future research should consider: responses when invited to participate; the role of methodological and organisational barriers to recruitment; the complexities of recruitment from a health professional perspective; developing culturally sensitive research methods; the magnitude of the problem of under

  4. South Asian Cluster

    OpenAIRE

    Ionel Sergiu Pirju

    2014-01-01

    This article aims at presenting the South Asian cluster composed of India, Indonesia, Iran and Malaysia, the intercultural values that characterizes it, the supported leadership style and tracing the main macroeconomic considerations which characterizes them. The research is synchronic, analysing the contemporary situation of these countries without reference to their evolution in time, by using the positivist paradigm that explains the reality at one point. It will be analysed th...

  5. Asian American Adolescent Identity

    OpenAIRE

    Ohm, Julie Juhye

    1999-01-01

    The formation of ego identity in Asian American late adolescents attending Virginia Tech was examined within the frameworks of Erikson's psychosocial theory and Berry, Trimble, and Olmedo's model of acculturation. Ego identity was measured using the Achieved sub-scale of the Revised Version of the Extended Objective Measure of Ego Identity Status, an instrument based on the theoretical constructs of Erikson. Ethnic identity was measured using the Multigroup Ethnic Identity Measure and America...

  6. Asian Media Productions

    DEFF Research Database (Denmark)

    This work consists of 12 essays on different aspects of Asian media by Japanese, European, and American scholars, many of whom have themselves been involved in the production of media forms. Working in the fields of anthropology, media and cultural studies, and on the basis of hands-on research......, they have written a book on the social practices and cultural attitudes of people producing, reading, watching and listening to different kinds of media in Japan, China, Taiwan, Indonesia, Vietnam, Singapore and India....

  7. Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol.

    Science.gov (United States)

    Kolosova, Anna Yu; De Saeger, Sarah; Sibanda, Liberty; Verheijen, Ron; Van Peteghem, Carlos

    2007-12-01

    A multianalyte lateral-flow technique using colloidal gold-labeled monoclonal antibodies was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEA). The results of this qualitative one-step test were interpreted visually. A very simple and fast sample preparation was used, and the assay procedure could be accomplished within 10 min. When applied to spiked wheat samples, the technique gave accurate and reproducible results. Cut-off levels of 1500 and 100 microg kg(-1) for DON and ZEA, respectively, were observed. The described multianalyte format can be used as a reliable, rapid and cost-effective on-site screening technique for the simultaneous determination of mycotoxins in grain samples.

  8. Assessing health systems for type 1 diabetes in sub-Saharan Africa: developing a 'Rapid Assessment Protocol for Insulin Access'

    Directory of Open Access Journals (Sweden)

    de Courten Maximilian

    2006-02-01

    Full Text Available Abstract Background In order to improve the health of people with Type 1 diabetes in developing countries, a clear analysis of the constraints to insulin access and diabetes care is needed. We developed a Rapid Assessment Protocol for Insulin Access, comprising a series of questionnaires as well as a protocol for the gathering of other data through site visits, discussions, and document reviews. Methods The Rapid Assessment Protocol for Insulin Access draws on the principles of Rapid Assessment Protocols which have been developed and implemented in several different areas. This protocol was adapted through a thorough literature review on diabetes, chronic condition management and medicine supply in developing countries. A visit to three countries in sub-Saharan Africa and meetings with different experts in the field of diabetes helped refine the questionnaires. Following the development of the questionnaires these were tested with various people familiar with diabetes and/or healthcare in developing countries. The Protocol was piloted in Mozambique then refined and had two further iterations in Zambia and Mali. Translations of questionnaires were made into local languages when necessary, with back translation to ensure precision. Results In each country the protocol was implemented in 3 areas – the capital city, a large urban centre and a predominantly rural area and their respective surroundings. Interviews were carried out by local teams trained on how to use the tool. Data was then collected and entered into a database for analysis. Conclusion The Rapid Assessment Protocol for Insulin Access was developed to provide a situational analysis of Type 1 diabetes, in order to make recommendations to the national Ministries of Health and Diabetes Associations. It provided valuable information on patients' access to insulin, syringes, monitoring and care. It was thus able to sketch a picture of the health care system with regards to its ability to

  9. Asian Rhinoplasty: Preoperative Simulation and Planning Using Adobe Photoshop.

    Science.gov (United States)

    Kiranantawat, Kidakorn; Nguyen, Anh H

    2015-11-01

    A rhinoplasty in Asians differs from a rhinoplasty performed in patients of other ethnicities. Surgeons should understand the concept of Asian beauty, the nasal anatomy of Asians, and common problems encountered while operating on the Asian nose. With this understanding, surgeons can set appropriate goals, choose proper operative procedures, and provide an outcome that satisfies patients. In this article the authors define the concept of an Asian rhinoplasty-a paradigm shift from the traditional on-top augmentation rhinoplasty to a structurally integrated augmentation rhinoplasty-and provide a step-by-step procedure for the use of Adobe Photoshop as a preoperative program to simulate the expected surgical outcome for patients and to develop a preoperative plan for surgeons.

  10. Ex vivo piperaquine resistance developed rapidly in Plasmodium falciparum isolates in northern Cambodia compared to Thailand

    Directory of Open Access Journals (Sweden)

    Suwanna Chaorattanakawee

    2016-10-01

    Full Text Available Abstract Background The recent dramatic decline in dihydroartemisinin-piperaquine (DHA-PPQ efficacy in northwestern Cambodia has raised concerns about the rapid spread of piperaquine resistance just as DHA-PPQ is being introduced as first-line therapy in neighbouring countries. Methods Ex vivo parasite susceptibilities were tracked to determine the rate of progression of DHA, PPQ and mefloquine (MQ resistance from sentinel sites on the Thai–Cambodian and Thai–Myanmar borders from 2010 to 2015. Immediate ex vivo (IEV histidine-rich protein 2 (HRP-2 assays were used on fresh patient Plasmodium falciparum isolates to determine drug susceptibility profiles. Results IEV HRP-2 assays detected the precipitous emergence of PPQ resistance in Cambodia beginning in 2013 when 40 % of isolates had an IC90 greater than the upper limit of prior years, and this rate doubled to 80 % by 2015. In contrast, Thai–Myanmar isolates from 2013 to 14 remained PPQ-sensitive, while northeastern Thai isolates appeared to have an intermediate resistance profile. The opposite trend was observed for MQ where Cambodian isolates appeared to have a modest increase in overall sensitivity during the same period, with IC50 declining to median levels comparable to those found in Thailand. A significant association between increased PPQ IC50 and IC90 among Cambodian isolates with DHA-PPQ treatment failure was observed. Nearly all Cambodian and Thai isolates were deemed artemisinin resistant with a >1 % survival rate for DHA in the ring-stage assay (RSA, though there was no correlation among isolates to indicate cross-resistance between PPQ and artemisinins. Conclusions Clinical DHA-PPQ failures appear to be associated with declines in the long-acting partner drug PPQ, though sensitivity appears to remain largely intact for now in western Thailand. Rapid progression of PPQ resistance associated with DHA-PPQ treatment failures in northern Cambodia limits drugs of choice in

  11. Ex vivo piperaquine resistance developed rapidly in Plasmodium falciparum isolates in northern Cambodia compared to Thailand.

    Science.gov (United States)

    Chaorattanakawee, Suwanna; Lon, Chanthap; Jongsakul, Krisada; Gawee, Jariyanart; Sok, Somethy; Sundrakes, Siratchana; Kong, Nareth; Thamnurak, Chatchadaporn; Chann, Soklyda; Chattrakarn, Sorayut; Praditpol, Chantida; Buathong, Nillawan; Uthaimongkol, Nichapat; Smith, Philip; Sirisopana, Narongrid; Huy, Rekol; Prom, Satharath; Fukuda, Mark M; Bethell, Delia; Walsh, Douglas S; Lanteri, Charlotte; Saunders, David

    2016-10-21

    The recent dramatic decline in dihydroartemisinin-piperaquine (DHA-PPQ) efficacy in northwestern Cambodia has raised concerns about the rapid spread of piperaquine resistance just as DHA-PPQ is being introduced as first-line therapy in neighbouring countries. Ex vivo parasite susceptibilities were tracked to determine the rate of progression of DHA, PPQ and mefloquine (MQ) resistance from sentinel sites on the Thai-Cambodian and Thai-Myanmar borders from 2010 to 2015. Immediate ex vivo (IEV) histidine-rich protein 2 (HRP-2) assays were used on fresh patient Plasmodium falciparum isolates to determine drug susceptibility profiles. IEV HRP-2 assays detected the precipitous emergence of PPQ resistance in Cambodia beginning in 2013 when 40 % of isolates had an IC90 greater than the upper limit of prior years, and this rate doubled to 80 % by 2015. In contrast, Thai-Myanmar isolates from 2013 to 14 remained PPQ-sensitive, while northeastern Thai isolates appeared to have an intermediate resistance profile. The opposite trend was observed for MQ where Cambodian isolates appeared to have a modest increase in overall sensitivity during the same period, with IC50 declining to median levels comparable to those found in Thailand. A significant association between increased PPQ IC50 and IC90 among Cambodian isolates with DHA-PPQ treatment failure was observed. Nearly all Cambodian and Thai isolates were deemed artemisinin resistant with a >1 % survival rate for DHA in the ring-stage assay (RSA), though there was no correlation among isolates to indicate cross-resistance between PPQ and artemisinins. Clinical DHA-PPQ failures appear to be associated with declines in the long-acting partner drug PPQ, though sensitivity appears to remain largely intact for now in western Thailand. Rapid progression of PPQ resistance associated with DHA-PPQ treatment failures in northern Cambodia limits drugs of choice in this region, and urgently requires alternative therapy. The temporary re

  12. Organizational innovation: a comprehensive model for catalyzing organizational development and change in a rapidly changing world

    National Research Council Canada - National Science Library

    Steiber, Annika; Alänge, Sverker

    2015-01-01

    ..., especially the processes through which organizational innovations are created, diffused, and sustained. There is thus a need for a more comprehensive understanding of mechanisms catalyzing organizational development...

  13. Biosimilars in Developed and Developing East and Southeast Asian Countries: Japan, South Korea, and Malaysia—Overview, Evolution, and Regulations Assessment

    Science.gov (United States)

    Bas, Tomas Gabriel; Oliu Castillo, Carolina

    2016-01-01

    The development of biological products has experienced continuous growth over the past three decades. The expiration of patent protection for many biological medicines has led to the development of biosimilars in many countries around the world. This paper reviews the literature on biosimilar drugs and covers their therapeutic status, clinical trials, approved biosimilars, and regulatory guidelines in Japan, South Korea, and Malaysia. The literature suggests that biosimilars are comparable but not identical to the reference product. They are not a generic version of an innovative product and do not ensure therapeutic equivalence. Biosimilars present more challenges than conventional generics and their marketing approval is also much more complicated. Guidelines for biosimilars were published in Japan in July 2009 by the Ministry of Health, Labour and Welfare (MHLW), in South Korea in March 2009 by the Ministry of Food and Drug Safety (MFDS), and in Malaysia in July 2008 by the National Pharmaceutical Control Bureau (NPCB). PMID:27213153

  14. Biosimilars in Developed and Developing East and Southeast Asian Countries: Japan, South Korea, and Malaysia—Overview, Evolution, and Regulations Assessment

    Directory of Open Access Journals (Sweden)

    Tomas Gabriel Bas

    2016-01-01

    Full Text Available The development of biological products has experienced continuous growth over the past three decades. The expiration of patent protection for many biological medicines has led to the development of biosimilars in many countries around the world. This paper reviews the literature on biosimilar drugs and covers their therapeutic status, clinical trials, approved biosimilars, and regulatory guidelines in Japan, South Korea, and Malaysia. The literature suggests that biosimilars are comparable but not identical to the reference product. They are not a generic version of an innovative product and do not ensure therapeutic equivalence. Biosimilars present more challenges than conventional generics and their marketing approval is also much more complicated. Guidelines for biosimilars were published in Japan in July 2009 by the Ministry of Health, Labour and Welfare (MHLW, in South Korea in March 2009 by the Ministry of Food and Drug Safety (MFDS, and in Malaysia in July 2008 by the National Pharmaceutical Control Bureau (NPCB.

  15. Developing a Natural Gas-Powered Bus Rapid Transit Service: A Case Study

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, G.

    2015-11-03

    The Roaring Fork Transit Authority (RFTA) and its VelociRFTA Bus Rapid Transit (BRT) program are unique in many ways. For example, VelociRFTA was the first rural BRT system in the United States and the operational environment of the VelociRFTA BRT is one of the most severe in the country, with extreme winter temperatures and altitudes close to 8,000 feet. RFTA viewed high altitude operation as the most challenging characteristic when it began considering the use of natural gas. RFTA is the second-largest public transit system in Colorado behind Denver's Regional Transportation District (RTD), and it is one of the largest rural public transit systems in the country. In 2013, RFTA accepted delivery of 22 new compressed natural gas (CNG) buses that went into service after completion of maintenance and refueling facilities earlier that year. This paper examines the lessons learned from RFTA's experience of investigating--and ultimately choosing--CNG for their new BRT program and focuses on the unique environment of RFTA's BRT application; the decision process to include CNG fueling in the project; unforeseen difficulties encountered in the operation of CNG buses; public perception; cost comparison to competing fuels; and considerations for indoor fueling facilities and project funding.

  16. Developing a Natural Gas-Powered Bus Rapid Transit Service. A Case Study

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, George [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-11-01

    The Roaring Fork Transit Authority (RFTA) and its VelociRFTA Bus Rapid Transit (BRT) program are unique in many ways. For example, VelociRFTA was the first rural BRT system in the United States and the operational environment of the VelociRFTA BRT is one of the most severe in the country, with extreme winter temperatures and altitudes close to 8,000 feet. RFTA viewed high altitude operation as the most challenging characteristic when it began considering the use of natural gas. RFTA is the second-largest public transit system in Colorado behind Denver's Regional Transportation District (RTD), and it is one of the largest rural public transit systems in the country. In 2013, RFTA accepted delivery of 22 new compressed natural gas (CNG) buses that went into service after completion of maintenance and refueling facilities earlier that year. This paper examines the lessons learned from RFTA's experience of investigating--and ultimately choosing--CNG for their new BRT program and focuses on the unique environment of RFTA's BRT application; the decision process to include CNG fueling in the project; unforeseen difficulties encountered in the operation of CNG buses; public perception; cost comparison to competing fuels; and considerations for indoor fueling facilities and project funding.

  17. Development of a rapid HRM genotyping method for detection of dog-derived Giardia lamblia.

    Science.gov (United States)

    Tan, Liping; Yu, Xingang; Abdullahi, Auwalu Yusuf; Wu, Sheng; Zheng, Guochao; Hu, Wei; Song, Meiran; Wang, Zhen; Jiang, Biao; Li, Guoqing

    2015-11-01

    Giardia lamblia is a zoonotic flagellate protozoan in the intestine of human and many mammals including dogs. To assess a threat of dog-derived G. lamblia to humans, the common dog-derived G. lamblia assemblages A, C, and D were genotyped by high-resolution melting (HRM) technology. According to β-giardin gene sequence, the qPCR-HRM primers BG5 and BG7 were designed. A series of experiments on the stability, sensitivity, and accuracy of the HRM method were also tested. Results showed that the primers BG5 and BG7 could distinguish among three assemblages A, C, and D, which Tm value differences were about 1 °C to each other. The melting curves of intra-assay reproducibility were almost coincided, and those of inter-assay reproducibility were much the same shape. The lowest detection concentration was about 5 × 10(-6)-ng/μL sample. The genotyping results from 21 G. lamblia samples by the HRM method were in complete accordance with sequencing results. It is concluded that the HRM genotyping method is rapid, stable, specific, highly sensitive, and suitable for clinical detection and molecular epidemiological survey of dog-derived G. lamblia.

  18. Development of a New Decision Tree to Rapidly Screen Chemical Estrogenic Activities of Xenopus laevis.

    Science.gov (United States)

    Wang, Ting; Li, Weiying; Zheng, Xiaofeng; Lin, Zhifen; Kong, Deyang

    2014-02-01

    During the last past decades, there is an increasing number of studies about estrogenic activities of the environmental pollutants on amphibians and many determination methods have been proposed. However, these determination methods are time-consuming and expensive, and a rapid and simple method to screen and test the chemicals for estrogenic activities to amphibians is therefore imperative. Herein is proposed a new decision tree formulated not only with physicochemical parameters but also a biological parameter that was successfully used to screen estrogenic activities of the chemicals on amphibians. The biological parameter, CDOCKER interaction energy (Ebinding ) between chemicals and the target proteins was calculated based on the method of molecular docking, and it was used to revise the decision tree formulated by Hong only with physicochemical parameters for screening estrogenic activity of chemicals in rat. According to the correlation between Ebinding of rat and Xenopus laevis, a new decision tree for estrogenic activities in Xenopus laevis is finally proposed. Then it was validated by using the randomly 8 chemicals which can be frequently exposed to Xenopus laevis, and the agreement between the results from the new decision tree and the ones from experiments is generally satisfactory. Consequently, the new decision tree can be used to screen the estrogenic activities of the chemicals, and combinational use of the Ebinding and classical physicochemical parameters can greatly improves Hong's decision tree. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Development of a Rapid and Simple Method to Remove Polyphenols from Plant Extracts

    Directory of Open Access Journals (Sweden)

    Imali Ranatunge

    2017-01-01

    Full Text Available Polyphenols are secondary metabolites of plants, which are responsible for prevention of many diseases. Polyvinylpolypyrrolidone (PVPP has a high affinity towards polyphenols. This method involves the use of PVPP column to remove polyphenols under centrifugal force. Standards of gallic acid, epigallocatechin gallate, vanillin, and tea extracts (Camellia sinensis were used in this study. PVPP powder was packed in a syringe with different quantities. The test samples were layered over the PVPP column and subjected to centrifugation. Supernatant was tested for the total phenol content. The presence of phenolic compounds and caffeine was screened by HPLC and measuring the absorbance at 280. The antioxidant capacity of standards and tea extracts was compared with the polyphenol removed fractions using DPPH scavenging assay. No polyphenols were found in polyphenolic standards or tea extracts after PVPP treatment. The method described in the present study to remove polyphenols is simple, inexpensive, rapid, and efficient and can be employed to investigate the contribution of polyphenols present in natural products to their biological activity.

  20. Development and evaluation of an office ergonomic risk checklist: ROSA--rapid office strain assessment.

    Science.gov (United States)

    Sonne, Michael; Villalta, Dino L; Andrews, David M

    2012-01-01

    The Rapid Office Strain Assessment (ROSA) was designed to quickly quantify risks associated with computer work and to establish an action level for change based on reports of worker discomfort. Computer use risk factors were identified in previous research and standards on office design for the chair, monitor, telephone, keyboard and mouse. The risk factors were diagrammed and coded as increasing scores from 1 to 3. ROSA final scores ranged in magnitude from 1 to 10, with each successive score representing an increased presence of risk factors. Total body discomfort and ROSA final scores for 72 office workstations were significantly correlated (R = 0.384). ROSA final scores exhibited high inter- and intra-observer reliability (ICCs of 0.88 and 0.91, respectively). Mean discomfort increased with increasing ROSA scores, with a significant difference occurring between scores of 3 and 5 (out of 10). A ROSA final score of 5 might therefore be useful as an action level indicating when immediate change is necessary. ROSA proved to be an effective and reliable method for identifying computer use risk factors related to discomfort. Copyright © 2011 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  1. EJS, JIL Server, and LabVIEW: An Architecture for Rapid Development of Remote Labs

    Science.gov (United States)

    Chacón, Jesús; Vargas, Hector; Farias, Gonzalo; Sanchez, José; Dormido, Sebastián

    2015-01-01

    Designing and developing web-enabled remote laboratories for pedagogical purposes is not an easy task. Often, developers (generally, educators who know the subjects they teach but lack of the technical and programming skills required to build Internet-based educational applications) end up discarding the idea of exploring these new teaching and…

  2. Low-Cost Rapid Usability Testing: Its Application in Both Product Development and System Implementation.

    Science.gov (United States)

    Kushniruk, Andre; Borycki, Elizabeth

    2017-01-01

    In recent years there has been considerable discussion around the need for certification and regulation of healthcare information technology (IT). In particular, the usability of the products being developed needs to be evaluated. This has included the application of standards designed to ensure the process of system development is user-centered and takes usability into consideration while a product is being developed. In addition to this, in healthcare, organizations in the United States and Europe have also addressed the need and requirement for product certification. However, despite these efforts there are continued reports of unusable and unsafe implementations. In this paper we discuss the need to not only include (and require) usability testing in the one-time development process of health IT products (such as EHRs), but we also argue for the need to additionally develop specific usability standards and requirements for usability testing during the implementation of vendor products (i.e. post product development) in healthcare settings. It is further argued that health IT products that may have been certified regarding their development process will still require application of usability testing in the process of implementing them in real hospital settings in order to ensure usability and safety. This is needed in order to ensure that the final result of both product development and implementation processes take into account and apply the latest usability principles and methods.

  3. Extended Services in Schools: Developing Resources to Prepare Student Teachers for a Rapidly Changing Working Environment

    Science.gov (United States)

    Griffiths, Sue; Smith, Andy

    2011-01-01

    The role of schools in providing extended services to their communities continues to undergo change and development. This has raised issues regarding the training of student teachers who are increasingly likely to take up appointments in schools offering extended services. This research project investigated the development of resources to prepare…

  4. Role of Helicobacter pylori cagA EPIYA motif and vacA genotypes for the development of gastrointestinal diseases in Southeast Asian countries: a meta-analysis

    Directory of Open Access Journals (Sweden)

    Sahara Shu

    2012-09-01

    Full Text Available Abstract Background Infection with cagA-positive, cagA EPIYA motif ABD type, and vacA s1, m1, and i1 genotype strains of Helicobacter pylori is associated with an exacerbated inflammatory response and increased risk of gastroduodenal diseases. However, it is unclear whether the prevalence and virulence factor genotypes found in Southeast Asia are similar to those in Western countries. Here, we examined the cagA status and prevalence of cagA EPIYA motifs and vacA genotypes among H. pylori strains found in Southeast Asia and examined their association with gastroduodenal disease. Methods To determine the cagA status, cagA EPIYA motifs, and vacA genotypes of H. pylori, we conducted meta-analyses of 13 previous reports for 1,281 H. pylori strains detected from several Southeast Asian countries. Results The respective frequencies of cagA-positive and vacA s1, m1, and i1 genotypes among examined subjects were 93% (1,056/1,133, 98% (1,010/1,033, 58% (581/1,009, and 96% (248/259, respectively. Stratification showed significant variation in the frequencies of cagA status and vacA genotypes among countries and the individual races residing within each respective country. The frequency of the vacA m-region genotype in patients infected with East Asian-type strains differed significantly between the northern and southern areas of Vietnam (p vacA m1 type or cagA-positive strains was associated with an increased risk of peptic ulcer disease (odds ratio: 1.46, 95%CI: 1.01-2.12, p = 0.046 and 2.83, 1.50-5.34, p = 0.001, respectively in the examined Southeast Asian populations. Conclusions Both Western- and East Asian-type strains of H. pylori are found in Southeast Asia and are predominantly cagA-positive and vacA s1 type. In Southeast Asia, patients infected with vacA m1 type or cagA-positive strains have an increased risk of peptic ulcer disease. Thus, testing for this genotype and the presence of cagA may have clinical usefulness.

  5. Shaping travels and land use with bus rapid transit: a developed city’s visualisation with smartcard and census data

    Directory of Open Access Journals (Sweden)

    Jiangping Zhou

    2016-01-01

    Full Text Available Transit and land-use integration is regarded as one of the most important means of avoiding or reducing car dependence and urban sprawl, which are thought as major culprits of unsustainability. In developing countries, rail rapid transit could be too expensive to become the predominant component of their respective systems. However, bus rapid transit (BRT as one of the cheapest forms of mass transit is a better alternative for those countries. In developing countries, BRT has transformed numerous cities such as Curitiba, Brazil; Bogota, Columbia; and Guangzhou, China. Can BRT have the same transformative impacts for cities in developed countries as well? Can the impacts of BRT be visualized using the transit population? Given that few cities in developed countries have BRT and ridership data of BRT are not always available, the above questions have not been well addressed before. To answer these two questions, we analysed smartcard swipes over a five-day weekday period (11–15 March 2013 for Brisbane in Queensland, Australia, and reconstructed trip trajectories of those 255,887 transit riders. We found that BRT serves a significant percentage and number of travellers, thus shaping travel behaviour and ultimately land use – when transit ridership and rate of transit usage are used as indicators. This is a significant achievement in Australia, a country known for its car dependence and urban sprawl.

  6. Rapid development of microsatellite markers for Callosobruchus chinensis using Illumina paired-end sequencing.

    Directory of Open Access Journals (Sweden)

    Can-Xing Duan

    Full Text Available BACKGROUND: The adzuki bean weevil, Callosobruchus chinensis L., is one of the most destructive pests of stored legume seeds such as mungbean, cowpea, and adzuki bean, which usually cause considerable loss in the quantity and quality of stored seeds during transportation and storage. However, a lack of genetic information of this pest results in a series of genetic questions remain largely unknown, including population genetic structure, kinship, biotype abundance, and so on. Co-dominant microsatellite markers offer a great resolving power to determine these events. Here, we report rapid microsatellite isolation from C. chinensis via high-throughput sequencing. PRINCIPAL FINDINGS: In this study, 94,560,852 quality-filtered and trimmed reads were obtained for the assembly of genome using Illumina paired-end sequencing technology. In total, the genome with total length of 497,124,785 bp, comprising 403,113 high quality contigs was generated with de novo assembly. More than 6800 SSR loci were detected and a suit of 6303 primer pair sequences were designed and 500 of them were randomly selected for validation. Of these, 196 pair of primers, i.e. 39.2%, produced reproducible amplicons that were polymorphic among 8 C. chinensis genotypes collected from different geographical regions. Twenty out of 196 polymorphic SSR markers were used to analyze the genetic diversity of 18 C. chinensis populations. The results showed the twenty SSR loci were highly polymorphic among these populations. CONCLUSIONS: This study presents a first report of genome sequencing and de novo assembly for C. chinensis and demonstrates the feasibility of generating a large scale of sequence information and SSR loci isolation by Illumina paired-end sequencing. Our results provide a valuable resource for C. chinensis research. These novel markers are valuable for future genetic mapping, trait association, genetic structure and kinship among C. chinensis.

  7. Development of a rapid, one-step screening method for the isolation of presumptive proteolytic enterococci.

    Science.gov (United States)

    Graham, Ken; Rea, Rosemary; Simpson, Paul; Stack, Helena

    2017-01-01

    Enterococci show higher proteolytic activities than other lactic acid bacteria and thus have received considerable attention in scientific literature in recent years. Proteolytic enzymes of enterococci have warranted the use of some species as starter, adjuncts or protective cultures and as probiotics, while in some strains they have also been linked with virulence. Consequently, the isolation and identification of proteolytic enterococci is becoming of increasing interest and importance. However, current screening methods for proteolytic enterococci can be time consuming, requiring a two-step procedure which may take up to 96h. This study describes a method, utilising Kanamycin Skim Milk Aesculin Azide (KSMEA) agar, for the isolation of proteolytic enterococci in one-step, thereby significantly reducing screening time. KSMEA combines the selective properties of Kanamycin Aesculin Azide Agar (KAA) with skim milk powder for the detection of proteolytic enterococci. Enterococci produced colonies with a black halo on KSMEA which were accompanied by a zone of clearing in the media when enterococci were proteolytic. KSMEA medium retained the selectivity of KAA, while proteolytic enterococci were easily distinguished from non-proteolytic enterococci when two known strains were propagated on KSMEA. KSMEA also proved effective at isolating and detecting enterococci in raw milk, faeces and soil. Isolates recovered from the screen were confirmed as enterococci using genus-specific primers. Proteolytic enterococci were present in the raw milk sample only and were easily distinguishable from non-proteolytic enterococci and other microorganisms. Therefore, KSMEA provides a rapid, one-step screening method for the isolation of presumptive proteolytic enterococci. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Rapid diagnosis of tuberculosis using Xpert MTB/RIF assay - Report from a developing country.

    Science.gov (United States)

    Iram, Shagufta; Zeenat, Asyia; Hussain, Shahida; Wasim Yusuf, Noshin; Aslam, Maleeha

    2015-01-01

    To evaluate the diagnostic accuracy of the Xpert MTB/RIF assay for the detection of M. tuberculosis in pulmonary and extrapulmonary specimens and to compare it with conventional techniques. During a period of 10 months from December 2012 through September 2013, two hundred and forty five clinically TB suspects were enrolled for Xpert MTB\\RIF assay. The cohort comprised of 205 suspects of pulmonary TB and 40 of extrapulmonary TB (EPTB). The 40 EPTB samples included pus aspirated from different sites of the body (n=19), pleural fluid (n=11), ascitic fluid (n=7), pericardial fluid, CSF and urine one each. Ziehl-Neelsen (ZN) Stained smear microscopy, culture on LJ media and Xpert MTB/RIF assay was performed on samples from these patients. M. tuberculosis (MTB) were detected by Xpert MTB/RIF test in 111 (45.3%) out of 245 samples. Of these, 85 (34.7%) were smear positive on ZN staining and 102 (41.6%) were positive on LJ cultures. Rifampicin resistance was detected in 16 (6.5%) patients. Nine out of 19 pus samples (47.3%) were positive for MTB by Gene Xpert, 03 (15.8%) on ZN staining and 04 (21%) on LJ culture. MTB could not be detected in any other extrapulmonary sample. Xpert MTB/RIF is a sensitive method for rapid diagnosis of Tuberculosis, especially in smear negative cases and in EPTB as compared to the conventional ZN staining. Among EPTB cases the highest yield of positivity was shown in Pus samples. For countries endemic for TB GeneXpert can serve as a sensitive and time saving diagnostic modality for pulmonary and EPTB.

  9. Selection for rapid embryo development correlates with embryo exposure to maternal androgens among passerine birds

    Science.gov (United States)

    Schwabl, H.; Palacios, M.G.; Martin, T.E.

    2007-01-01

    Greater offspring predation favors evolution of faster development among species. We hypothesized that greater offspring predation exerts selection on mothers to increase levels of anabolic androgens in egg yolks to achieve faster development. Here, we tested whether (1) concentrations of yolk androgens in passerine species were associated with offspring predation and (2) embryo and nestling development rates were associated with yolk androgen concentrations. We examined three androgens that increase in potency along the synthesis pathway: androstenedione (A4) to testosterone (T) to 5??- dihydrotestosterone (5??-DHT). Concentrations of none of these steroids were related to clutch size; only A4 was allometrically related to egg volume. Species that experience greater predation showed higher yolk concentrations of T and 5??-DHT. Higher concentrations of T and particularly 5??-DHT were strongly correlated with faster development during the embryo period and less so during the nestling period. Development rates were most strongly correlated with 5??-DHT, suggesting that potency increases along the androgen synthesis pathway and that effects are mediated by the androgen receptor pathway. These results are consistent with the hypothesis that selection for faster development by time-dependent offspring mortality may be achieved epigenetically by varying embryo exposure to maternal anabolic steroids. ?? 2007 by The University of Chicago. All rights reserved.

  10. Infant Feeding Practices of Emirati Women in the Rapidly Developing City of Abu Dhabi, United Arab Emirates

    Directory of Open Access Journals (Sweden)

    Hazel Gardner

    2015-09-01

    Full Text Available Rapid economic and cultural transition in the United Arab Emirates (UAE has been accompanied by new challenges to public health; most notably a rapid rise in chronic disease. Breastfeeding is known to improve health outcomes in adulthood, is associated with reduced risk of developing chronic disease, and is therefore an important public health issue for this rapidly increasing population. Factors associated with infant feeding practices were examined in a cohort of 125 Emirati women and their infants, with data collected at birth and 3, 6 and 15 months postpartum by questionnaires and interviews. Participants were recruited in the Corniche Hospital, the main maternity hospital in the city of Abu Dhabi. Factors affecting the duration of breastfeeding and the introduction of complementary foods were investigated using univariate and multivariate statistics. Recommended infant feeding practices, such as exclusive breastfeeding for the first six months of life and timely introduction of appropriate complementary foods, were poorly adhered to. Factors implicated in early cessation of breastfeeding included: time to first breastfeed, mother’s education level, employment status and early introduction of complementary foods.

  11. Development of a Rapid Microbore Metabolic Profiling Ultraperformance Liquid Chromatography-Mass Spectrometry Approach for High-Throughput Phenotyping Studies.

    Science.gov (United States)

    Gray, Nicola; Adesina-Georgiadis, Kyrillos; Chekmeneva, Elena; Plumb, Robert S; Wilson, Ian D; Nicholson, Jeremy K

    2016-06-07

    A rapid gradient microbore ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) method has been developed to provide a high-throughput analytical platform for the metabolic phenotyping of urine from large sample cohorts. The rapid microbore metabolic profiling (RAMMP) approach was based on scaling a conventional reversed-phase UPLC-MS method for urinary profiling from 2.1 mm × 100 mm columns to 1 mm × 50 mm columns, increasing the linear velocity of the solvent, and decreasing the gradient time to provide an analysis time of 2.5 min/sample. Comparison showed that conventional UPLC-MS and rapid gradient approaches provided peak capacities of 150 and 50, respectively, with the conventional method detecting approximately 19 000 features compared to the ∼6 000 found using the rapid gradient method. Similar levels of repeatability were seen for both methods. Despite the reduced peak capacity and the reduction in ions detected, the RAMMP method was able to achieve similar levels of group discrimination as conventional UPLC-MS when applied to rat urine samples obtained from investigative studies on the effects of acute 2-bromophenol and chronic acetaminophen administration. When compared to a direct infusion MS method of similar analysis time the RAMMP method provided superior selectivity. The RAMMP approach provides a robust and sensitive method that is well suited to high-throughput metabonomic analysis of complex mixtures such as urine combined with a 5-fold reduction in analysis time compared with the conventional UPLC-MS method.

  12. The rapid bi-level exploration on the evolution of regional solar energy development

    Science.gov (United States)

    Guan, Qing; An, Haizhong; Li, Huajiao; Hao, Xiaoqing

    2017-01-01

    As one of the renewable energy, solar energy is experiencing increased but exploratory development worldwide. The positive or negative influences of regional characteristics, like economy, production capacity and allowance policies, make them have uneven solar energy development. In this paper, we aim at quickly exploring the features of provincial solar energy development, and their concerns about solar energy. We take China as a typical case, and combine text mining and two-actor networks. We find that the classification of levels based on certain nodes and the amount of degree avoids missing meaningful information that may be ignored by global level results. Moreover, eastern provinces are hot focus for the media, western countries are key to bridge the networks and special administrative region has local development features; third, most focus points are more about the application than the improvement of material. The exploration of news provides practical information to adjust researches and development strategies of solar energy. Moreover, the bi-level exploration, which can also be expanded to multi-level, is helpful for governments or researchers to grasp more targeted and precise knowledge.

  13. USGS science and technology help managers battle invading Asian carp

    Science.gov (United States)

    Kolar, Cynthia S.; Morrison, Sandra S.

    2016-09-28

    The U.S. Geological Survey (USGS) conducts Asian carp research focused on early detection, risk assessment, and development of control tools and strategies. The goals are to prevent the establishment of invasive Asian carp in the Great Lakes and to reduce their impacts in the Ohio River and Mississippi River Basins and elsewhere. Managers can use the information, tools, and strategies for early detection of Asian carp and to control them when their presence is first evident. New detection and control tools are designed to accommodate expansion to other invasive species and application in geographically diverse areas.This USGS focus complements goals of the Great Lakes Restoration Initiative (GLRI), a multi-agency collaboration started in 2010 to protect and restore the Great Lakes. As a member of the Asian Carp Regional Coordinating Committee, which guides Asian carp efforts, the USGS works closely with Federal and State agencies, Canada, and others to address high-priority Asian carp issues and provide science to inform management decisions.The USGS has gained extensive knowledge of Asian carp biology and life history over the past 30 years. That knowledge guides the design, development, and application of control strategies, and is essential for developing approaches in line with modern principles and practices of integrated pest management (IPM). IPM is a process used to solve pest problems while minimizing risks to people and the environment.

  14. The MetSkill Program — Rapidly Developing Effective Young Engineers in the Workplace

    Science.gov (United States)

    Drinkwater, Diana; Bianco, Nina

    MetSkill is a professional development program for metallurgical engineers that integrates with normal duties in their first one or two years of service. Graduates work together on a structured technical project, facilitated by specialists and supported by formal learning, and ultimately reported to their technical managers. The program enables graduates to "fill the gaps" in their undergraduate education, which is increasingly pertinent as engineering degrees become more general. Participants report that they enjoy the focus on more challenging (rewarding) aspects of their jobs and feel more confident about problem solving. Sponsor companies add that the relationships developed with external technical specialists enhances opportunities for innovation and development. MetSkill was delivered to two major resource companies in Australia in 2012. This paper provides an outline of the program and the reasons for its success, and demonstrates how the learning model could be applied to groups of graduates in other engineering disciplines.

  15. Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.

    Science.gov (United States)

    García, Alfredo; Martínez, Remigio; Benitez-Medina, José Manuel; Risco, David; Garcia, Waldo Luis; Rey, Joaquín; Alonso, Juan Manuel; Hermoso de Mendoza, Javier

    2013-01-01

    Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.

  16. Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

    OpenAIRE

    García, Alfredo; Martínez, Remigio; Benitez-Medina, José Manuel; Risco, David; García, Waldo Luis; Rey, Joaquín; Alonso, Juan Manuel; de Mendoza, Javier Hermoso

    2013-01-01

    Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was ...

  17. Development of loop-mediated isothermal amplification (LAMP assay for rapid and sensitive identification of ostrich meat.

    Directory of Open Access Journals (Sweden)

    Amir Abdulmawjood

    Full Text Available Animal species identification is one of the primary duties of official food control. Since ostrich meat is difficult to be differentiated macroscopically from beef, therefore new analytical methods are needed. To enforce labeling regulations for the authentication of ostrich meat, it might be of importance to develop and evaluate a rapid and reliable assay. In the present study, a loop-mediated isothermal amplification (LAMP assay based on the cytochrome b gene of the mitochondrial DNA of the species Struthio camelus was developed. The LAMP assay was used in combination with a real-time fluorometer. The developed system allowed the detection of 0.01% ostrich meat products. In parallel, a direct swab method without nucleic acid extraction using the HYPLEX LPTV buffer was also evaluated. This rapid processing method allowed detection of ostrich meat without major incubation steps. In summary, the LAMP assay had excellent sensitivity and specificity for detecting ostrich meat and could provide a sampling-to-result identification-time of 15 to 20 minutes.

  18. Rapid breeding and varietal replacement are critical to adaptation of cropping systems in the developing world to climate change.

    Science.gov (United States)

    Atlin, Gary N; Cairns, Jill E; Das, Biswanath

    2017-03-01

    Plant breeding is a key mechanism for adaptation of cropping systems to climate change. Much discussion of breeding for climate change focuses on genes with large effects on heat and drought tolerance, but phenology and stress tolerance are highly polygenic. Adaptation will therefore mainly result from continually adjusting allele frequencies at many loci through rapid-cycle breeding that delivers a steady stream of incrementally improved cultivars. This will require access to elite germplasm from other regions, shortened breeding cycles, and multi-location testing systems that adequately sample the target population of environments. The objective of breeding and seed systems serving smallholder farmers should be to ensure that they use varieties developed in the last 10 years. Rapid varietal turnover must be supported by active dissemination of new varieties, and active withdrawal of obsolete ones. Commercial seed systems in temperate regions achieve this through competitive seed markets, but in the developing world, most crops are not served by competitive commercial seed systems, and many varieties date from the end of the Green Revolution (the late 1970s, when the second generation of modern rice and wheat varieties had been widely adopted). These obsolete varieties were developed in a climate different than today's, placing farmers at risk. To reduce this risk, a strengthened breeding system is needed, with freer international exchange of elite varieties, short breeding cycles, high selection intensity, wide-scale phenotyping, and accurate selection supported by genomic technology. Governments need to incentivize varietal release and dissemination systems to continuously replace obsolete varieties.

  19. Extinction, Reacquisition, and Rapid Forgetting of Eyeblink Conditioning in Developing Rats

    Science.gov (United States)

    Brown, Kevin L.; Freeman, John H.

    2014-01-01

    Eyeblink conditioning is a well-established model for studying the developmental neurobiology of associative learning and memory. However, age differences in extinction and subsequent reacquisition have yet to be studied using this model. The present study examined extinction and reacquisition of eyeblink conditioning in developing rats. In…

  20. Developing Rapid and Cost-Effective Tools for Assessing Groundwater Impacts on Contaminated Sediments

    Science.gov (United States)

    This research developed quick and inexpensive methods that can be useful in characterizing the interaction of water and solids within the GW/SW transition zone to explain processes that occur during physical contact between groundwater and sediments. The research used self-conta...

  1. Service platform for rapid development and deployment of context-Aware, mobile applications

    NARCIS (Netherlands)

    Pokraev, S.; Koolwaaij, J.; van Setten, M.; Broens, T.H.F.; Dockhorn Costa, P.; Wibbels, M.; Ebben, P.; Strating, P.; Strating, P.

    2005-01-01

    In this paper we present a web services-based platform that facilitates and speeds up the development and deployment of context-aware, integrated mobile speech and data applications. The platform is capable of handling different types of context and offers sophisticated personalization mechanisms.

  2. EMF Rapid Program Engineering Projects, Project 1, Development of Recommendations for Guidelines for Field Source Measurement

    Energy Technology Data Exchange (ETDEWEB)

    Electric Research and Management, Inc.

    1997-03-11

    The goal of this project is to develop a protocol for measuring the electric and magnetic fields around sources. Data from these measurements may help direct future biological effects research by better defining the complexity of magnetic and electric fields to which humanity is exposed, as well asprovide the basis for rigorous field exposure analysis and risk assessment once the relationship between field exposure and biological response. is better understood. The data base also should have sufficient spatial and temporal characteristics to guide electric and magnetic field management. The goal of Task A is to construct a set of characteristics that would be ideal to have for guiding and interpreting biological studies and for focusing any future effort at field management. This ideal set will then be quantified and reduced according to the availability (or possible development of) instrumentation to measure the desired characteristics. Factors that also will be used to define pragmatic data sets will be the cost of collecting the data, the cost of developing an adequate data base, and the needed precision in measuring specific characteristics. A field, electric or magnetic, will always be ,some function of time and space. The first step in this section of the protocol development will be to determine what span of time and what portion of space are required to quantify the electric and magnetic fields around sources such as appliances and electrical apparatus. Constraints on time will be set by examining measurement limitations and biological data requirements.

  3. Recent developments in the rapid analysis of plants and tracking their bioactive constituents

    NARCIS (Netherlands)

    Beek, van T.A.; Tetala, K.K.R.; Koleva, I.; Dapkevicius, A.; Exarchou, V.; Jeurissen, S.M.F.; Claassen, F.W.; Klift, van der E.J.C.

    2009-01-01

    Natural products chemistry has witnessed many new developments in the last 5 years like extractions with subcritical water and ionic liquids, LC/HRMS and LC/SPE/cryo-NMR, UHPLC, TLC/MS, MS-based preparative HPLC, comprehensive chromatography (GC × GC, LC × LC), high-throughput screening,

  4. A Survey of Hardware and Software Technologies for the Rapid Development of Multimedia Instructional Modules

    Science.gov (United States)

    Ganesan, Nanda

    2008-01-01

    A survey of hardware and software technologies was conducted to identify suitable technologies for the development of instructional modules representing various instructional approaches. The approaches modeled were short PowerPoint presentations, chalk-and-talk type of lectures and software tutorials. The survey focused on identifying application…

  5. Rapid development of bromodomain probes using flow synthesis methods and frontal affinity chromatography

    OpenAIRE

    Ingham, Richard; Guetzoyan, Lucie; Nikbin, Nikzad; Ley, Steven V.

    2014-01-01

    This poster illustrates aspects of a project involving the development of new probes for bromodomain 9 protein. Flow chemistry technologies and remote monitoring techniques were used for the synthesis, and Frontal Affinity Chromatography assays were used for analysis of the products.

  6. Using INGRES as a rapid prototyping device during development of management information applications

    Energy Technology Data Exchange (ETDEWEB)

    Brice, L.; Connell, J.; Shafer, D.

    1983-01-01

    This paper presents case studies from the Administrative Data Processing Division of the Los Alamos National Laboratory where a prototyping too, the INGRES relational database system, has been used to develop management information systems. The tool has proved valuable in satisfying user requirements and expectations, and in aiding data processing in the analysis and specification phases of the system life cycle. The prototype approach helps enormously in bridging the developer-user communication gap and has been found to add a negligible amount of cost to the entire software development project. Presented here are four case studies of how INGRES has been employed in prototyping. Also presented are examples of specific INGRES features and how they were used in one of the case studies and further examples involving another similar case. Special considerations and cautions are required when using INGRES for prototyping, but the overall conclusion is that it is a tool which has tremendously benefited our organization. Whether the final implemented system is INGRES-based or not, prototyping greatly enhances the possibility of complete, correct and unambiguous specifications prior to final software product development.

  7. DEVELOPMENT OF NOVEL CERAMIC NANOFILM-FIBER INTEGRATED OPTICAL SENSORS FOR RAPID DETECTION OF COAL DERIVED SYNTHESIS GAS

    Energy Technology Data Exchange (ETDEWEB)

    Junhang Dong; Hai Xiao; Xiling Tang; Hongmin Jiang; Kurtis Remmel; Amardeep Kaur

    2012-09-30

    The overall goal of this project is to conduct fundamental studies on advanced ceramic materials and fiber optic devices for developing new types of high temperature (>500{degree}C) fiber optic chemical sensors (FOCS) for monitoring fossil (mainly coal) and biomass derived gases in power plants. The primary technical objective is to investigate and demonstrate the nanocrystalline doped-ceramic thin film enabled FOCS that possess desired stability, sensitivity and selectivity for in-situ, rapid gas detection in the syngas streams from gasification and combustion flue gases. This report summarizes research works of two integrated parts: (1) development of metal oxide solid thin films as sensing materials for detection and measurement of important gas components relevant to the coal- and biomass-derived syngas and combustion gas streams at high temperatures; and (2) development of fiber optic devices that are potentially useful for constructing FOCS in combination with the solid oxide thin films identified in this program.

  8. Colorado Plateau Rapid Ecoregion Assessment Change Agents - Development - Current, Near-Term, and Long-Term Potential High Landscape Development

    Data.gov (United States)

    Bureau of Land Management, Department of the Interior — This map shows areas of high current, near-term, and long-term potential landscape development, based on factors such as urban areas, agriculture, roads, and energy...

  9. Towards an ASEAN Human Rights Framework : Rapid Development in the Kingdom of Cambodia

    OpenAIRE

    Camilla Marie, Mårtensson

    2016-01-01

    The Association of Southeast Asia Nations (ASEAN) has since its origins in the late 1960’s been connected to the emphasis on Economic, Social and Cultural rights and the regional need for economic development. Through the intense years and debates that followed the first ASEAN Declaration the unity has made significant contributions to the Human Rights discourse due to the challenges and possibilities that surfaced. The Kingdom of Cambodia is one of the ASEAN member states poorest countries b...

  10. Development and evaluation of loop-mediated isothermal amplification assay for rapid detection of Capripoxvirus

    OpenAIRE

    Kanisht Batra; Aman Kumar; Vinay Kumar; Trilok Nanda; Maan, Narender S.; Sushila Maan

    2015-01-01

    Aim: The present study was undertaken to develop a nucleic acid-based diagnostic assay loop-mediated isothermal amplification assay (LAMP) targeting highly conserved genomic regions of Capripoxvirus (CaPVs) and its comparative evaluation with real-time polymerase chain reaction (PCR). Material and Methods: Lyophilized vaccine strain of sheeppox virus (SPPV) was used for optimization of LAMP assay. The LAMP assay was designed using envelope immunogenic protein (P32) coding gene targeting highl...

  11. Framework for rapid in-house development of web applications for higher education institutions in Poland

    OpenAIRE

    Mincer-Daszkiewicz, Janina

    2013-01-01

    1. ABSTRACTUSOS [2] is a student management information system developed in-house for higher educationinstitutions (HEIs) in Poland, from the MUCI consortium. It consists of a desktop applicationdeveloped in Oracle technology for the university administration, and a suite of web basedapplications, for handling admission, course registration, grades, student diploma theses, social aid,course surveys and many other services, dedicated for students and university teachers. They weredeveloped in ...

  12. Mobile Application Development for Optimal and Rapid Diagnosis of Vine Diseases

    Directory of Open Access Journals (Sweden)

    Liliana Lucia TOMOIAGĂ

    2017-05-01

    Recent developments in the field of machine learning (Ghahramani, 2015, breakthroughs in computer vision (Krizhevsky et al., 2012 and the availability of cheap computational hardware has led us to look for a way to automatically detect and classify a disease just by having a computer or a mobile phone process a picture of an affected plant. Given the widespread availability of mobile devices, we believe we can help the vine growers further.

  13. Rapid development of cognitive maps in people with visual impairments when exploring novel geographic spaces

    OpenAIRE

    Jacobson, Dan; Lippa, Yvonne; Golledge, Reginald G.; Kitchin, Rob; Blades, Mark

    2001-01-01

    'Cognitive' map is a term that refers to a person's environmental knowledge. Anyone experiencing a new environment will, over time, develop a cognitive representation of that environment, including information derived from that environment (e.g., about places, routes and spatial relationships) and information about personal experiences (e.g. memories about events at locations and attitudes towards places). There is now a great deal of research into the cognitive maps of sighted people (see Go...

  14. Investigation of Capabilities and Technologies Supporting Rapid UAV Launch System Development

    Science.gov (United States)

    2015-06-01

    OF PAGES 169 16. PRICE CODE 17. SECURITY CLASSIFICATION OF REPORT Unclassified 18. SECURITY CLASSIFICATION OF THIS PAGE Unclassified 19. SECURITY ...Development System LCD liquid-crystal display xv LED light-emitting diode NPS Naval Postgraduate School RATO rocket assisted take-Off RFID radio frequency...platform interface by the AMPPS’s radio frequency identification ( RFID ) reader • Green Light–A launch has been initiated by the technician and is either

  15. Study on the change and acculturation of dietary pattern of Southeast Asian workers living in South Korea.

    Science.gov (United States)

    Lee, Eun Jung; Lee, Kyung-Ran; Lee, Seung-Joo

    2017-10-01

    This study analyzed the dietary pattern of Southeast Asian workers (Vietnamese, Thais, Cambodians and Myanmar) living in South Korea in order to recognize the dietary changes after they moved to South Korea. Questionnaires were completed by 251 Southeast Asian workers living in South Korea. Using a self-administered questionnaire, we assessed the diets before and after living in the hometown and in South Korea. Significant changes observed in the Southeast Asian workers were decreased in consumption frequency of fresh fruits, cooked vegetables, rice noodles, green tea and glutinous rice, and increase in consumption of Kimchi, seaweed, milk, coffee and pizza. These changes were attributed to rapid dietary acculturation. The frequencies of eating homemade food were significantly decreased after they came to Korea except for Thais. Thais showed the highest frequencies of eating homemade food daily among others. 28.2% of respondents said their health condition had deteriorated after living in South Korea due to difficulties to adapt Korean food, increased frequencies of eating instant food, and lacking exercises. By providing understanding of the dietary patterns of Southeast Asian workers, these results can be used for preliminary data to develop a program for their Korean food adaptation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Rapid torque development in older female fallers and nonfallers: a comparison across lower-extremity muscles.

    Science.gov (United States)

    LaRoche, Dain P; Cremin, Kim A; Greenleaf, Brittnee; Croce, Ronald V

    2010-06-01

    The objective of this study was to compare reaction time, joint torque, rate of torque development, and magnitude of neuromuscular activation of lower-extremity muscles in elderly female fallers and nonfallers. Participants included 11, elderly, female fallers (71.3+/-5.4 years) and twelve nonfallers (71.3+/-6.2 years) who completed a fall risk questionnaire. Then, maximal, voluntary, isometric contractions of the knee and ankle muscles were performed in reaction to a visual cue to determine joint torque, rate of torque development, reaction time, and nervous activation of agonists and antagonists. Results indicated that significantly more fallers reported "dizziness upon rising", "use of balance altering medications", "stress or depression", "not enough sleep", "arthritis in lower body", "chronic pain in lower body", and "tiring easily while walking" (all Ptorque, rate of torque development and impulse) were lower in fallers than in nonfallers (Pmuscle groups, fallers demonstrated 19% lower peak torque and 29% longer motor time (P<0.05). In conclusion, comprehensive fall risk screening and prevention programs should address both neuromuscular and non-muscular factors, and, weakness of the ankle dorsiflexors and plantarflexors should be further studied as potential contributors to falls in older adults. (c) 2009 Elsevier Ltd. All rights reserved.

  17. Learning from marketing: Rapid development of medication messages that engage patients.

    Science.gov (United States)

    Yank, Veronica; Tribett, Erika; Green, Lydia; Pettis, Jasmine

    2015-08-01

    To adapt marketing approaches in a health services environment. Researchers and advertising professionals partnered in developing advertising-style messages designed to activate patients pre-identified as having chronic kidney disease to ask providers about recommended medications. We assessed feasibility of the development process by evaluating partnership structure, costs, and timeframe. We tested messages with patients and providers using preliminary surveys to refine initial messages and subsequent focus groups to identify the most persuasive ones. The partnership achieved an efficient structure, $14,550 total costs, and 4-month timeframe. The advertising team developed 11 initial messages. The research team conducted surveys and focus groups with a total of 13 patients and 8 providers to identify three messages as most activating. Focus group themes suggested the general approach of using advertising-style messages was acceptable if it supported patient-provider relationships and had a credible evidence base. Individual messages were more motivating if they elicited personal identification with imagery, particular emotions, active patient role, and message clarity. We demonstrated feasibility of a research-advertising partnership and acceptability and likely impact of advertising-style messages on patient medication-seeking behavior. Healthcare systems may want to replicate our adaptation of marketing approaches to patients with chronic conditions. Copyright © 2015. Published by Elsevier Ireland Ltd.

  18. RAPID COMMUNICATION: Nerve growth factor influences cleavage rate and embryo development in sheep.

    Science.gov (United States)

    Crispo, M; Dos Santos-Neto, P C; Vilariño, M; Mulet, A P; de León, A; Barbeito, L; Menchaca, A

    2016-10-01

    Recent information about Nerve growth factor (NGF), a protein traditionally associated to the nervous system that regulates survival and maturation of developing neurons, suggests that it may exert action also on different levels in the reproductive system. The aim of this study was to evaluate the effect of NGF added during in vitro oocyte maturation, fertilization or in vitro embryo development in sheep. Nerve growth factor was supplemented to the culture medium at 0, 100, or 1,000 ng/mL, during either in vitro maturation (Exp. 1), in vitro fertilization (Exp. 2), or in vitro culture (Exp. 3). In addition, NGF mRNA expression was determined in cumulus cells and oocytes. Nerve growth factor induced early cleavage when added during oocyte maturation or fertilization, improved embryo development when added during fertilization, and had no significant effect when added during embryo culture. In general, the effect was more evident with 100 rather than 1,000 ng/mL (P growth factor on oocyte maturation and mainly on the fertilization process.

  19. Development of rapidly dissolving pellets within the Quality by Design approach.

    Science.gov (United States)

    Karatzas, A A; Politis, S N; Rekkas, D M

    2017-05-01

    The purpose of this study was the development of novel, fast disintegrating, effervescent pellets by employing the direct pelletization technique as a single step process. In line with the Quality by Design (QbD) regulatory framework, statistical experimental design was extensively applied to correlate significant formulation and process variables with the critical quality attributes of the product. Pellets were studied with regards to sphericity, size and size distribution. In contrast to the existing multiparticulate platforms, this development integrated only water-soluble excipients to facilitate the multifunctional use of the final dosage form. The application of a screening fractional factorial design augmented to a full factorial design set the roadmap for the rational selection of the composition and process parameters, revealing in parallel the positive contribution of the powder feeder on the CQAs, when the critical process and formulation factors were properly adjusted. The response surface methodology was exploited for the final process optimization phase, which allowed the construction of appropriate mathematical models connecting the input variables and the CQAs under study. The implementation of the desirability function, lead to the optimum formulation and process settings for the production of pellets with narrow size distribution and geometric mean diameter of approximately 800 μm. In conclusion, using a lean approach supported by design of experiments (DoE) techniques within the QbD framework, a novel multifunctional formulation platform has been developed.

  20. Developing a More Rapid Test to Assess Sulfate Resistance of Hydraulic Cements

    Science.gov (United States)

    Ferraris, Chiara; Stutzman, Paul; Peltz, Max; Winpigler, John

    2005-01-01

    External sulfate attack of concrete is a major problem that can appear in regions where concrete is exposed to soil or water containing sulfates, leading to softening and cracking of the concrete. Therefore, it is important that materials selection and proportioning of concrete in susceptible regions be carefully considered to resist sulfate attack. American Society for Testing Materials (ASTM) limits the tricalcium aluminate phase in cements when sulfate exposure is of concern. The hydration products of tricalcium aluminate react with the sulfates resulting in expansion and cracking. While ASTM standard tests are available to determine the susceptibility of cements to sulfate attack, these tests require at least 6 months and often up to a year to perform; a delay that hinders development of new cements. This paper presents a new method for testing cement resistance to sulfate attack that is three to five times faster than the current ASTM tests. Development of the procedure was based upon insights on the degradation process by petrographic examination of sulfate-exposed specimens over time. Also key to the development was the use of smaller samples and tighter environmental control. PMID:27308177

  1. Final Report on the Proposal to Provide Asian Science and Technology Information

    Energy Technology Data Exchange (ETDEWEB)

    Kahaner, David K. [Asian Technology Information Program

    2003-07-23

    The Asian Technology Information Program (ATIP) conducted a seven-month Asian science and technology information program for the Office:of Energy Research (ER), U.S: Department of Energy (DOE.) The seven-month program consists of 1) monitoring, analyzing, and dissemiuating science and technology trends and developments associated with Asian high performance computing and communications (HPC), networking, and associated topics, 2) access to ATIP's annual series of Asian S&T reports for ER and HPC related personnel and, 3) supporting DOE and ER designated visits to Asia to study and assess Asian HPC.

  2. Method Development for Rapid Analysis of Natural Radioactive Nuclides Using Sector Field Inductively Coupled Plasma Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Lim, J.M.; Ji, Y.Y.; Lee, H.; Park, J.H.; Jang, M.; Chung, K.H.; Kang, M.J.; Choi, G.S. [Korea Atomic Energy Research Institute (Korea, Republic of)

    2014-07-01

    As an attempt to reduce the social costs and apprehension arising from radioactivity in the environment, an accurate and rapid assessment of radioactivity is highly desirable. Naturally occurring radioactive materials (NORM) are widely spread throughout the environment. The concern with radioactivity from these materials has therefore been growing for the last decade. In particular, radiation exposure in the industry when handling raw materials (e.g., coal mining and combustion, oil and gas production, metal mining and smelting, mineral sands (REE, Ti, Zr), fertilizer (phosphate), and building materials) has been brought to the public's attention. To decide the proper handling options, a rapid and accurate analytical method that can be used to evaluate the radioactivity of radionuclides (e.g., {sup 238}U, {sup 235}U, {sup 232}Th, {sup 226}Ra, and {sup 40}K) should be developed and validated. Direct measuring methods such as alpha spectrometry, a liquid scintillation counter (LSC), and mass-spectrometry are usually used for the measurement of radioactivity in NORM samples, and they encounter the most significant difficulties during pretreatment (e.g., purification, speciation, and dilution/enrichment). Since the pretreatment process consequently plays an important role in the measurement uncertainty, method development and validation should be performed. Furthermore, a-spectrometry has a major disadvantage of a long counting time, while it has a prominent measurement capability at a very low activity level of {sup 238}U, {sup 235}U, {sup 232}Th, and {sup 226}Ra. Contrary to the α-spectrometry method, a measurement technique using ICP-MS allow radioactivity in many samples to be measured in a short time period with a high degree of accuracy and precision. In this study, a method was developed for a rapid analysis of natural radioactive nuclides using ICP-MS. A sample digestion process was established using LiBO{sub 2} fusion and Fe co-precipitation. A magnetic

  3. Suggestions for an East Asian Monetary Union: Focusing on the Effects of Monetary Integration on Bilateral Trade

    Directory of Open Access Journals (Sweden)

    Min-Chang Ko

    2006-06-01

    Full Text Available This study explores alternative ways toward the East Asian monetary union, focusing on the effect of monetary integration on bilateral trade. For this purpose, we analyze with gravity model changes of the bilateral trade among countries of EU, NAFTA, and East Asia. A distinctive feature of this model is that it includes the dummy variables that show the cultural intimacy among North-East Asian countries, Asian NIEs countries, and East Asian countries (North-East Asian and ASEAN countries. We discovered that bilateral trade increased at a highest level among East Asian countries than the other two cases. This result suggests that the once-and-for-all strategy to develop an East Asian monetary union including North-East Asian and ASEAN countries would be more efficient than the multitrack strategy that forms the monetary union of North-East Asian countries in the first place and then tries to extend it to ASEAN countries.

  4. South Asian Cluster

    Directory of Open Access Journals (Sweden)

    Ionel Sergiu Pirju

    2014-12-01

    Full Text Available This article aims at presenting the South Asian cluster composed of India, Indonesia, Iran and Malaysia, the intercultural values that characterizes it, the supported leadership style and tracing the main macroeconomic considerations which characterizes them. The research is synchronic, analysing the contemporary situation of these countries without reference to their evolution in time, by using the positivist paradigm that explains the reality at one point. It will be analysed the overall cluster with the existing interactions between the countries that composes it, while the article being one of information will avoid building recommendation, or new theories.

  5. Global Recession and Asian Growth: Experience and Prospects

    OpenAIRE

    Dowling, John Malcolm

    2009-01-01

    The paper outlines current macroeconomic developments in industrial countries and explains how slower growth in these economies is being transmitted to developing economies in Asia. The macroeconomic outlook for industrial countries in 2009 is discussed along with the transmission mechanism that has brought the global downturn tothe Asian economies. Monetary and fiscal policy adjustments in Asian economies have been implemented to address the downturn in economic activity and these policies a...

  6. GLOBAL RECESSION AND ASIAN GROWTH: EXPERIENCE AND PROSPECTS

    OpenAIRE

    Dowling, John Malcolm

    2015-01-01

    The paper outlines current macroeconomic developments in industrial countries and explains how slower growth in these economies is being transmitted to developing economies in Asia. The macroeconomic outlook for industrial countries in 2009 is discussed along with the transmission mechanism that has brought the global downturn tothe Asian economies. Monetary and fiscal policy adjustments in Asian economies have been implemented to address the downturn in economic activity and these policies a...

  7. Development and evaluation of rapid novel isothermal amplification assays for important veterinary pathogens: Chlamydia psittaci and Chlamydia pecorum

    Directory of Open Access Journals (Sweden)

    Martina Jelocnik

    2017-09-01

    Full Text Available Background Chlamydia psittaci and Chlamydia pecorum are important veterinary pathogens, with the former also being responsible for zoonoses, and the latter adversely affecting koala populations in Australia and livestock globally. The rapid detection of these organisms is still challenging, particularly at the point-of-care (POC. In the present study, we developed and evaluated rapid, sensitive and robust C. psittaci-specific and C. pecorum-specific Loop Mediated Isothermal Amplification (LAMP assays for detection of these pathogens. Methods and Materials The LAMP assays, performed in a Genie III real-time fluorometer, targeted a 263 bp region of the C. psittaci-specific Cps_0607 gene or a 209 bp region of a C. pecorum-specific conserved gene CpecG_0573, and were evaluated using a range of samples previously screened using species-specific quantitative PCRs (qPCRs. Species-specificity for C. psittaci and C. pecorum LAMP targets was tested against DNA samples from related chlamydial species and a range of other bacteria. In order to evaluate pathogen detection in clinical samples, C. psittaci LAMP was evaluated using a total of 26 DNA extracts from clinical samples from equine and avian hosts, while for C. pecorum LAMP, we tested a total of 63 DNA extracts from clinical samples from koala, sheep and cattle hosts. A subset of 36 C. pecorum samples was also tested in a thermal cycler (instead of a real-time fluorometer using newly developed LAMP and results were determined as an end point detection. We also evaluated rapid swab processing (without DNA extraction to assess the robustness of these assays. Results Both LAMP assays were demonstrated to species-specific, highly reproducible and to be able to detect as little as 10 genome copy number/reaction, with a mean amplification time of 14 and 24 min for C. psittaci and C. pecorum, respectively. When testing clinical samples, the overall congruence between the newly developed LAMP assays and q

  8. Development and evaluation of rapid novel isothermal amplification assays for important veterinary pathogens: Chlamydia psittaci and Chlamydia pecorum.

    Science.gov (United States)

    Jelocnik, Martina; Islam, Md Mominul; Madden, Danielle; Jenkins, Cheryl; Branley, James; Carver, Scott; Polkinghorne, Adam

    2017-01-01

    Chlamydia psittaci and Chlamydia pecorum are important veterinary pathogens, with the former also being responsible for zoonoses, and the latter adversely affecting koala populations in Australia and livestock globally. The rapid detection of these organisms is still challenging, particularly at the point-of-care (POC). In the present study, we developed and evaluated rapid, sensitive and robust C. psittaci-specific and C. pecorum-specific Loop Mediated Isothermal Amplification (LAMP) assays for detection of these pathogens. The LAMP assays, performed in a Genie III real-time fluorometer, targeted a 263 bp region of the C. psittaci-specific Cps_0607 gene or a 209 bp region of a C. pecorum-specific conserved gene CpecG_0573, and were evaluated using a range of samples previously screened using species-specific quantitative PCRs (qPCRs). Species-specificity for C. psittaci and C. pecorum LAMP targets was tested against DNA samples from related chlamydial species and a range of other bacteria. In order to evaluate pathogen detection in clinical samples, C. psittaci LAMP was evaluated using a total of 26 DNA extracts from clinical samples from equine and avian hosts, while for C. pecorum LAMP, we tested a total of 63 DNA extracts from clinical samples from koala, sheep and cattle hosts. A subset of 36 C. pecorum samples was also tested in a thermal cycler (instead of a real-time fluorometer) using newly developed LAMP and results were determined as an end point detection. We also evaluated rapid swab processing (without DNA extraction) to assess the robustness of these assays. Both LAMP assays were demonstrated to species-specific, highly reproducible and to be able to detect as little as 10 genome copy number/reaction, with a mean amplification time of 14 and 24 min for C. psittaci and C. pecorum, respectively. When testing clinical samples, the overall congruence between the newly developed LAMP assays and qPCR was 92.3% for C. psittaci (91.7% sensitivity and 92

  9. Immunizations and Asians and Pacific Islanders

    Science.gov (United States)

    ... and Data > Minority Population Profiles > Asian American > Immunizations Immunizations and Asians and Pacific Islanders Asian/Pacific Islander ... 35 months reached the Healthy People goal for immunizations for hepatitis B, MMR (measles-mumps-rubella), polio ...

  10. GSL: An Open Source Framework for the Rapid Development of Data Archive Access Services

    Science.gov (United States)

    McWhirter, J.; Boler, F. M.; Bock, Y.; Squibb, M. B.; Ratzesberger, L.

    2011-12-01

    The Geodetic Seamless Archive Center (GSAC) project is a NASA ROSES ACCESS funded effort with the goal to define and implement a web service API for uniform querying across multiple geodetic data repositories. The project information technology partners include two International GNSS Service (IGS) Data Centers, CDDIS and SOPAC, and a third global NSF and NASA-funded Data Center, UNAVCO. A science partner, the University of Nevada, Reno Geodetic Laboratory is utilizing the services and contributing new quality assessment products. Each of the participant data centers has its own internal database schema and various web-based access mechanisms to suit the needs of their individual user communities. The software engineering challenge that we faced was to unify these access mechanisms into a common web service API. To facilitate the development of the GSAC interfaces within these disparate repositories we have developed a Java-based open source middleware framework called the GSAC Service Layer (GSL). It was recognized early on in the project that much of the required functionality within each repository could be provided by a common software layer, thus achieving a substantial reduction in the development effort required by each partner institution. The GSL provides a range of common services for repository configuration, initialization, database management, web interface generation, and data model and metadata creation. Each repository implementation then simply focuses on the core task of responding to query requests and delivering to the GSL layer the internal results. The GSL layer is then responsible for encoding the results in the desired format. A fundamental challenge in developing a framework such as the GSL is balancing the trade-off between the agnosticism needed within the GSL so that it is applicable to a wide range of problem domains and the specific needs required for a particular repository implementations. The GSL addresses this challenge through an

  11. Development of Rapid Pipe Moulding Process for Carbon Fiber Reinforced Thermoplastics by Direct Resistance Heating

    Science.gov (United States)

    Tanaka, Kazuto; Harada, Ryuki; Uemura, Toshiki; Katayama, Tsutao; Kuwahara, Hideyuki

    To deal with environmental issues, the gasoline mileage of passenger cars can be improved by reduction of the car weight. The use of car components made of Carbon Fiber Reinforced Plastics (CFRP) is increasing because of its superior mechanical properties and relatively low density. Many vehicle structural parts are pipe-shaped, such as suspension arms, torsion beams, door guard bars and impact beams. A reduction of the car weight is expected by using CFRP for these parts. Especially, when considering the recyclability and ease of production, Carbon Fiber Reinforced Thermoplastics are a prime candidate. On the other hand, the moulding process of CFRTP pipes for mass production has not been well established yet. For this pipe moulding process an induction heating method has been investigated already, however, this method requires a complicated coil system. To reduce the production cost, another system without such complicated equipment is to be developed. In this study, the pipe moulding process of CFRTP using direct resistance heating was developed. This heating method heats up the mould by Joule heating using skin effect of high-frequency current. The direct resistance heating method is desirable from a cost perspective, because this method can heat the mould directly without using any coils. Formerly developed Non-woven Stitched Multi-axial Cloth (NSMC) was used as semi-product material. NSMC is very suitable for the lamination process due to the fact that non-crimp stitched carbon fiber of [0°/+45°/90°/-45°] and polyamide 6 non-woven fabric are stitched to one sheet, resulting in a short production cycle time. The use of the pipe moulding process with the direct resistance heating method in combination with the NSMC, has resulted in the successful moulding of a CFRTP pipe of 300 mm in length, 40 mm in diameter and 2 mm in thickness.

  12. Non-obese diabetic mice rapidly develop dramatic sympathetic neuritic dystrophy: a new experimental model of diabetic autonomic neuropathy.

    Science.gov (United States)

    Schmidt, Robert E; Dorsey, Denise A; Beaudet, Lucie N; Frederick, Kathy E; Parvin, Curtis A; Plurad, Santiago B; Levisetti, Matteo G

    2003-11-01

    To address the pathogenesis of diabetic autonomic neuropathy, we have examined the sympathetic nervous system in non-obese diabetic (NOD) and streptozotocin (STZ)-induced diabetic mice, two models of type 1 diabetes, and the db/db mouse, a model of type 2 diabetes. After only 3 to 5 weeks of diabetes, NOD mice developed markedly swollen axons and dendrites ("neuritic dystrophy") in the prevertebral superior mesenteric and celiac ganglia (SMG-CG), similar to the pathology described in diabetic STZ- and BBW-rat and man. Comparable changes failed to develop in the superior cervical ganglia of the NOD mouse or in the SMG-CG of non-diabetic NOD siblings. STZ-induced diabetic mice develop identical changes, although at a much slower pace and to a lesser degree than NOD mice. NOD-SCID mice, which are genetically identical to NOD mice except for the absence of T and B cells, do not develop diabetes or neuropathology comparable to diabetic NOD mice. However, STZ-treated NOD-SCID mice develop severe neuritic dystrophy, evidence against an exclusively autoimmune pathogenesis for autonomic neuropathy in this model. Chronically diabetic type 2 db/db mice fail to develop neuritic dystrophy, suggesting that hyperglycemia alone may not be the critical and sufficient element. The NOD mouse appears to be a valuable model of diabetic sympathetic autonomic neuropathy with unambiguous, rapidly developing neuropathology which corresponds closely to the characteristic pathology of other rodent models and man.

  13. Development of a lateral flow immunoassay for the rapid diagnosis of invasive candidiasis

    OpenAIRE

    Zhengxin He; Lanchun Shi; Xiangyang Ran; Wei Li; Xianling Wang; Fukun Wang

    2016-01-01

    Early and accurate diagnosis of invasive candidiasis (IC) is very important. In this study, a lateral flow immunoassay (LFIA) was developed to detect antibody against Candida albicans enolase (Eno). Colloidal gold particle labeled mouse anti human IgG (1.0 mg/L) was used as the detector reagent. Recombinant enolase (rEno, 1.0 mg/L) and goat anti IgG (1.0 mg/L) were immobilized in test and control lines, respectively, of a nitrocellulose membrane, acting as the capture reagents. The LFIA was u...

  14. Development of microLIPS (Luciferase Immunoprecipitation Systems): a novel microfluidic assay for rapid serum antibody detection

    Science.gov (United States)

    Chandrangsu, Matt; Burbelo, Peter D.; Iadarola, Michael J.; Smith, Paul D.; Morgan, Nicole Y.

    2012-06-01

    There is considerable interest in the development of rapid, point-of-care antibody detection for the diagnosis of infectious and auto-immune diseases. In this paper, we present work on the development of a self-contained microfluidic format for the Luciferase Immunoprecipitation Systems (LIPS) assay. Whereas the majority of immunoassays for antigen-specific antibodies employ either bacteria- or yeast-expressed proteins and require the use of secondary antibodies, the LIPS technique uses a fusion protein comprised of a Renilla luciferase reporter and the antigen of interest produced via mammalian cell culture, ensuring the addition of mammalian post-translational modifications. Patient serum is mixed with the fusion protein and passed over immobilized Protein A/G; after washing, the only remaining luciferase-tagged antigens are those retained by specific antibodies. These can be quantitatively measured using chemiluminescence upon the introduction of coelenterazine. The assay has been successfully employed for a wide variety of diseases in a microwell format. We report on a recent demonstration of rapid HSV-2 diagnosis with the LIPS assay in a microfluidic format, using one microliter of serum and obtaining results in under ten minutes. We will also discuss recent progress on two fronts, both aimed at the deployment of this technology in the field: first, simplifying assay operation through the automation of flow control using power-free means; and second, efforts to increase signal levels, primarily through strategies to increase antibody binding capacity, in order to move towards portable battery powered electronics.

  15. Development of rapidly quenched nickel-based non-boron filler metals for brazing corrosion resistant steels

    Science.gov (United States)

    Ivannikov, A.; Kalin, B.; Suchkov, A.; Penyaz, M.; Yurlova, M.

    2016-04-01

    Corrosion-resistant steels are stably applied in modern rocket and nuclear technology. Creating of permanent joints of these steels is a difficult task that can be solved by means of welding or brazing. Recently, the use rapidly quenched boron-containing filler metals is perspective. However, the use of such alloys leads to the formation of brittle borides in brazing zone, which degrades the corrosion resistance and mechanical properties of the compounds. Therefore, the development of non-boron alloys for brazing stainless steels is important task. The study of binary systems Ni-Be and Ni-Si revealed the perspective of replacing boron in Ni-based filler metals by beryllium, so there was the objective of studying of phase equilibrium in the system Ni-Be-Si. The alloys of the Ni-Si-Be with different contents of Si and Be are considered in this paper. The presence of two low-melting components is revealed during of their studying by methods of metallography analysis and DTA. Microhardness is measured and X-ray diffraction analysis is conducted for a number of alloys of Ni-Si-Be. The compositions are developed on the basis of these data. Rapidly quenched brazing alloys can be prepared from these compositions, and they are suitable for high temperature brazing of steels.

  16. Development of an ELISA for rapid detection of anti-type VII collagen autoantibodies in epidermolysis bullosa acquisita.

    Science.gov (United States)

    Chen, M; Chan, L S; Cai, X; O'Toole, E A; Sample, J C; Woodley, D T

    1997-01-01

    Epidermolysis bullosa acquisita (EBA) is an acquired blistering skin disease characterized by the presence of IgG autoantibodies to type VII collagen. EBA autoantibodies recognize four major immunodominant epitopes localized within the amino-terminal, noncollagenous (NC1) domain. In this study, we developed a rapid, quantitative enzyme-linked immunosorbent assay (ELISA) to detect autoantibody activity against the complete NC1 domain of type VII collagen with the use of an eukaryotic-expressed, recombinant human NC1 antigen. With the ELISA, we tested serum from patients with EBA (n = 24), bullous systemic lupus erythematosus (BSLE) (n = 3), bullous pemphigoid (n = 16), pemphigus (n = 11), and normal controls (n = 12). All EBA and BSLE serum, including four sera that were negative by indirect immunofluorescence, demonstrated reactivity with immobilized NC1 in the ELISA. In contrast, none of the sera from healthy control subjects or patients with unrelated blistering skin diseases reacted with NC1. The EBA sera also reacted with recombinant NC1 by immunoblot analysis but with less sensitivity. Thus, the newly developed ELISA using recombinant NC1 is a sensitive, specific assay and a useful tool for rapidly screening EBA and BSLE serum.

  17. Development of an immunochromatographic strip test for rapid detection of melamine in raw milk, milk products and animal feed.

    Science.gov (United States)

    Li, Xiangmei; Luo, Pengjie; Tang, Shusheng; Beier, Ross C; Wu, Xiaoping; Yang, Lili; Li, Yanwei; Xiao, Xilong

    2011-06-08

    A simple, rapid and sensitive immunogold chromatographic strip test based on a monoclonal antibody was developed for the detection of melamine (MEL) residues in raw milk, milk products and animal feed. The limit of detection was estimated to be 0.05 μg/mL in raw milk, since the detection test line on the strip test completely disappeared at this concentration. The limit of detection was 2 μg/mL (or 2 μg/g) for milk drinks, yogurt, condensed milk, cheese, and animal feed and 1 μg/g for milk powder. Sample pretreatment was simple and rapid, and the results can be obtained within 3-10 min. A parallel analysis of MEL in 52 blind raw milk samples conducted by gas chromatography-mass spectrometry showed comparable results to those obtained from the strip test. The results demonstrate that the developed method is suitable for the onsite determination of MEL residues in a large number of samples.

  18. Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

    Directory of Open Access Journals (Sweden)

    Joojin Jeong

    2015-09-01

    Full Text Available The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR to demonstrate its advantages over RT-PCR. RT-LAMP reactions were conducted with or without a set of loop primers since one out of six primers showed PVX specificity. Based on real-time monitoring, RT-LAMP detected PVX around 30 min, compared to 120 min for RT-PCR. By adding a fluorescent reagent during the reaction, the extra step of visualization by gel electrophoresis was not necessary. RT-LAMP was conducted using simple inexpensive instruments and a regular incubator to evaluate whether RNA could be amplified at a constant temperature instead of using an expensive thermal cycler. This study shows the potential of RT-LAMP for the diagnosis of viral diseases and PVX epidemiology because of its simplicity and rapidness compared to RT-PCR.

  19. Assessment on the Impact of Arable Land Protection Policies in a Rapidly Developing Region

    Directory of Open Access Journals (Sweden)

    Jiadan Li

    2016-05-01

    Full Text Available To investigate the effect of arable land protection policies in China, a practical framework that integrates geographic information systems (GIS, soil quality assessment and landscape metrics analysis was employed to track and analyze arable land transformations and landscape changes in response to rampant urbanization within the Ningbo region (China from 2005 to 2013. The results showed that arable land loss and degradation have continued, despite the development of a comprehensive legal framework for arable land protection. The implementation of arable land protection policies is judged to be effective, but not entirely successful, because it guarantees the overall amount of arable land but does not consider soil quality and spatial distribution. In addition, there are distinct variations in arable land change dynamics between two temporal intervals. From 2005–2009, the transformation of arable land was diversified, with intensified conversion among arable land, built-up land, water and orchards. Moreover, many new arable land parcels were adjacent to built-up land, and are in danger of being occupied again through urban sprawl. By 2009–2013, most of the arable land was occupied by urban expansion, whereas a majority of newly increased arable land was reclaimed from coastal tideland. Although the newly increased arable land was contiguous and far from the urban area, it is of poor quality and has limited use. The permanent loss of high-quality arable land due to intensified urban sprawl may threaten sustainable development and food security on a larger scale.

  20. Ultrasensitive Silicon Nanowire Sensor Developed by a Special Ag Modification Process for Rapid NH3 Detection.

    Science.gov (United States)

    Qin, Yuxiang; Liu, Diao; Zhang, Tianyi; Cui, Zhen

    2017-08-30

    Surface functionalization is very effective in enhancing sensing properties of a chemiresistive gas sensor. In this work, we develop a novel and cost-effective process to prepare Ag-modified silicon nanowire (SiNW) sensors and further suggest a resistance effect model to clarify the enhanced sensing mechanism of Ag-modified SiNWs. The SiNWs were formed via metal-assisted chemical etching (MACE), and the Ag nanoparticle (NP) modification was achieved in situ based on the MACE-produced Ag dendrites by involving a crucial anisotropic postetching of TMAH. The TMAH etching induces a loose array of needle-like, rough SiNWs (RNWs) with firm attachment of tiny Ag NPs. Comparative investigations for NH3-sensing properties indicate that the RNWs modified by discrete Ag NPs (Ag@RNWs) display an ∼3-fold enhancement in gas response at room temperature compared with pristine SiNWs. Meanwhile, transient response and ultrafast recovery are observed for the Ag@RNW sensor (tres ≤ 2 s and trec ≤ 9 s to 0.33-10 ppm of NH3). The study demonstrates the considerable effect and potential of the Ag modification process developed in this work. A resistance effect model was further suggested to clarify the underlying mechanism of the enhanced response and the response saturation characteristic of the Ag@RNWs. The promotion of TMAH etching-induced microstructure modulation to sensing properties was also demonstrated.

  1. Rapid Sequestration of Leishmania mexicana by Neutrophils Contributes to the Development of Chronic Lesion.

    Directory of Open Access Journals (Sweden)

    Benjamin P Hurrell

    2015-05-01

    Full Text Available The protozoan Leishmania mexicana parasite causes chronic non-healing cutaneous lesions in humans and mice with poor parasite control. The mechanisms preventing the development of a protective immune response against this parasite are unclear. Here we provide data demonstrating that parasite sequestration by neutrophils is responsible for disease progression in mice. Within hours of infection L. mexicana induced the local recruitment of neutrophils, which ingested parasites and formed extracellular traps without markedly impairing parasite survival. We further showed that the L. mexicana-induced recruitment of neutrophils impaired the early recruitment of dendritic cells at the site of infection as observed by intravital 2-photon microscopy and flow cytometry analysis. Indeed, infection of neutropenic Genista mice and of mice depleted of neutrophils at the onset of infection demonstrated a prominent role for neutrophils in this process. Furthermore, an increase in monocyte-derived dendritic cells was also observed in draining lymph nodes of neutropenic mice, correlating with subsequent increased frequency of IFNγ-secreting T helper cells, and better parasite control leading ultimately to complete healing of the lesion. Altogether, these findings show that L. mexicana exploits neutrophils to block the induction of a protective immune response and impairs the control of lesion development. Our data thus demonstrate an unanticipated negative role for these innate immune cells in host defense, suggesting that in certain forms of cutaneous leishmaniasis, regulating neutrophil recruitment could be a strategy to promote lesion healing.

  2. Maximizing opportunities and mitigating risks in Canada's rapidly developing clean coal market

    Energy Technology Data Exchange (ETDEWEB)

    Tse, D.W. [GE Energy, Houston, TX (United States)

    2006-07-01

    The power generation industry faces many challenges. Across the U.S., 132 coal plants to deliver 82 GW of power are proposed. IGCC and advanced PC/SCPC are compared. Data is presented to show the environmental benefits of IGCC. Advantages of IGCC include a proven technology, fuel flexibility, good emissions performance, high efficiency, comparable cost of electricity at launch, energy security, and public acceptance. GE acquired CVX gasification technology in 2004 and formed the IGCC alliance with Bechtel later that year. The key requirements for an IGCC reference plant are outlined: plant performance, design status, reference plant options, capital costs, project phases, multiple offerings, global activities, development budget, gasification programmes, and future plans. 18 figs.

  3. Conditions on Early Mars Might Have Fostered Rapid and Early Development of Life

    Science.gov (United States)

    Gibson, Everett K.; McKay, David S.; Thomas-Keprta, Kathie L.; Clemett, Simon J.; Wentworth, Susan J.

    2007-01-01

    The exploration of Mars during the past decades has begun to unveil the history of the planet. The combinations of remote sensing, in situ geochemical compositional measurements and photographic observations from both above and on the surface have shown Mars to have a dynamic and active geologic evolution. Mars geologic evolution clearly had conditions that were suitable for supporting life. For a planet to be able to be habitable, it must have water, carbon sources, energy sources and a dynamic geologic past. Mars meets all of these requirements. The first 600 My of Martian history were ripe for life to develop because of the abundance of (i) Water-carved canyons and oceans or lakes with the early presence of near surface water shown by precipitated carbonates in ALH84001 well-dated at approx.3.9 Gy., (ii) Energy from the original accretional processes, a molten core which generated a strong magnetic field leaving a permanent record in the early crust, early active volcanism continuing throughout Martian history, and, and continuing impact processes, (iii) Carbon and water from possibly extensive volcanic outgassing (i.e. H2O, CO2, CH4, CO, O2, N2, H2S, SO2, etc.) and (iv) some crustal tectonics as revealed by faulting and possible plate movement reflected by the magnetic pattern in the crust. The question arises: "Why would life not evolve from these favorable conditions on early Mars in its first 600 My?" During this period, it seems likely that environmental near-surface conditions on Mars were more favorable to life than at any later time. Standing bodies of water, precipitation and flowing surface water, and possibly abundant hydrothermal energy would all favor the formation of early life. Even if life developed elsewhere (on Earth, Venus, or on other solar systems) and was transported to Mars, the surface conditions were likely very hospitable for that introduced life to multiply and evolve.

  4. Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons: the D:A:D study

    NARCIS (Netherlands)

    Kamara, David A.; Ryom, Lene; Ross, Michael; Kirk, Ole; Reiss, Peter; Morlat, Philippe; Moranne, Olivier; Fux, Christoph A.; Mocroft, Amanda; Sabin, Caroline; Lundgren, Jens D.; Smith, Colette J.; Powderly, B.; Shortman, N.; Moecklinghoff, C.; Reilly, G.; Franquet, X.; Ryom, L.; Sabin, C. A.; Kamara, D.; Smith, C.; Phillips, A.; Mocroft, A.; Tverland, J.; Mansfeld, M.; Nielsen, J.; Raben, D.; Lundgren, J. D.; Brandt, R. Salbøl; Rickenbach, M.; Fanti, I.; Krum, E.; Hillebregt, M.; Geffard, S.; Sundström, A.; Delforge, M.; Fontas, E.; Torres, F.; McManus, H.; Wright, S.; Kjær, J.; Sjøl, A.; Meidahl, P.; Helweg-Larsen, J.; Iversen, J. Schmidt; Kirk, O.; Ross, M.; Fux, C. A.; Morlat, P.; Moranne, O.; Kesselring, A. M.; Kamara, D. A.; Weber, R.; Pradier, C.; Friis-Møller, N.; Kowalska, J.; Sabin, C.; Law, M.; d'Arminio Monforte, A.; Dabis, F.; Bruyand, M.; Bower, M.; Fätkenheuer, G.; Donald, A.; Grulich, A.; Zaheri, S.; Gras, L.; Prins, J. M.; Kuijpers, T. W.; Scherpbier, H. J.; van der Meer, J. T. M.; Wit, F. W. M. N.; Godfried, M. H.; van der Poll, dr T.; Nellen, F. J. B.; Lange, J. M. A.; Geerlings, S. E.; van Vugt, M.; Pajkrt, D.; Bos, Drs J. C.; van der Valk, Drs M.; Grijsen, M. L.; Wiersinga, W. J.; Goorhuis, A.; Hovius, J. W. R.; Lowe, S.; Oude Lashof, A.; Posthouwer, D.; Ammerlaan, H. S. M.; Pronk, M. J. H.; van der Ende, M. E.; de Vries-Sluijs, T. E. M. S.; Schurink, C. A. M.; Nouwen, J. L.; Verbon, A.; Rijnders, B. J. A.; van Gorp, E. C. M.; van der Feltz, M.; Driessen, G. J. A.; van Rossum, A. M. C.; Branger, J.; Schippers, F.; van Nieuwkoop, C.; van Elzakker, E. P.; Groeneveld, P. H. P.; Bouwhuis, J. W.; Soetekouw, R.; ten Kate, R. W.; Kroon, F. P.; van Dissel, J. T.; Arend, S. M.; de Boer, M. G. J.; Jolink, H.; ter Vollaard, H. J. M.; Bauer, M. P.; den Hollander, J. G.; Pogany, K.; van Twillert, Drs G.; Kortmann, W.; Stuart, J. W. T. Cohen; Diederen, B. M. W.; Leyten, M. S.; Gelinck, L. B. S.; Kootstra, G. J.; Delsing, C. E.; Brinkman, K.; Blok, W. L.; Frissen, P. H. J.; Schouten, W. E. M.; van den Berk, G. E. L.; van Kasteren, M. E. E.; Brouwer, A. E.; Veenstra, J.; Lettinga, K. D.; Mulder, J. W.; Vrouenraets, S. M. E.; Lauw, F. N.; van Eeden, A.; Verhagen, D. W. M.; Sprenger, H. G.; Doedens, R.; Scholvinck, E. H.; van Assen, S.; Bierman, W. F. W.; Koopmans, P. P.; Keuter, M.; van der Ven, A. J. A. M.; ter Hofstede, H. J. M.; Dofferhoff, A. S. M.; Warris, A.; van Crevel, R.; Hoepelman, A. I. M.; Mudrikova, T.; Schneider, M. M. E.; Ellerbroek, P. M.; Oosterheert, J. J.; Arends, J. E.; Wassenberg, M. W. M.; Barth, R. E.; van Agtmael, M. A.; Perenboom, R. M.; Claessen, F. A. P.; Bomers, M.; Peters, E. J. G.; Geelen, S. P. M.; Wolfs, T. F. W.; Bont, L. J.; Richter, C.; van der Berg, J. P.; Gisolf, E. H.; van den Berge, M.; Stegeman, A.; van Vonderen, M. G. A.; van Houte, D. P. F.; Weijer, S.; el Moussaoui, R.; Winkel, C.; Muskiet, F.; Durand, N. N.; Voigt, R.; Chêne, G.; Lawson-Ayayi, S.; Thiébaut, R.; Bonnal, F.; Bonnet, F.; Bernard, N.; Caunègre, L.; Cazanave, C.; Ceccaldi, J.; Chambon, D.; Chossat, I.; Dauchy, F. A.; de Witte, S.; Dupon, M.; Duffau, P.; Dutronc, H.; Farbos, S.; Gaborieau, V.; Gemain, M. C.; Gerard, Y.; Greib, C.; Hessamfar, M.; Lacoste, D.; Lataste, P.; Lafarie, S.; Lazaro, E.; Malvy, D.; Meraud, J. P.; Mercié, P.; Monlun, E.; Neau, D.; Ochoa, A.; Pellegrin, J. L.; Pistone, T.; Ragnaud, J. M.; Receveur, M. C.; Tchamgoué, S.; Vandenhende, M. A.; Viallard, J. F.; Moreau, J. F.; Pellegrin, I.; Fleury, H.; Lafon, M. E.; Masquelier, B.; Trimoulet, P.; Breilh, D.; Haramburu, F.; Miremont-Salamé, G.; Blaizeau, M. J.; Decoin, M.; Delaune, J.; Delveaux, S.; D'Ivernois, C.; Hanapier, C.; Leleux, O.; Uwamaliya-Nziyumvira, B.; Sicard, X.; Palmer, G.; Touchard, D.; Petoumenos, K.; Bendall, C.; Moore, R.; Edwards, S.; Hoy, J.; Watson, K.; Roth, N.; Nicholson, J.; Bloch, M.; Franic, T.; Baker, D.; Vale, R.; Carr, A.; Cooper, D.; Chuah, J.; Ngieng, M.; Nolan, D.; Skett, J.; Calvo, G.; Mateu, S.; Domingo, P.; Sambeat, M. A.; Gatell, J.; del Cacho, E.; Cadafalch, J.; Fuster, M.; Codina, C.; Sirera, G.; Vaqué, A.; de Wit, S.; Clumeck, N.; Necsoi, C.; Gennotte, A. F.; Gerard, M.; Kabeya, K.; Konopnicki, D.; Libois, A.; Martin, C.; Payen, M. C.; Semaille, P.; van Laethem, Y.; Neaton, J.; Bartsch, G.; El-Sadr, W. M.; Thompson, G.; Wentworth, D.; Luskin-Hawk, R.; Telzak, E.; Abrams, D. I.; Cohn, D.; Markowitz, N.; Arduino, R.; Mushatt, D.; Friedland, G.; Perez, G.; Tedaldi, E.; Fisher, E.; Gordin, F.; Crane, L. R.; Sampson, J.; Baxter, J.; Lundgren, J.; Cozzi-Lepri, A.; Grint, D.; Podlekareva, D.; Peters, L.; Reekie, J.; Fischer, A. H.; Losso, M.; Elias, C.; Vetter, N.; Zangerle, R.; Karpov, I.; Vassilenko, A.; Mitsura, V. M.; Suetnov, O.; Colebunders, R.; Vandekerckhove, L.; Hadziosmanovic, V.; Kostov, K.; Machala, L.; Begovac, J.; Jilich, D.; Sedlacek, D.; Kronborg, G.; Gerstoft, J.; Benfield, T.; Larsen, M.; Katzenstein, T.; Hansen, A.-B. E.; Skinhøj, P.; Pedersen, C.; Ostergaard, L.; Zilmer, K.; Smidt, Jelena; Ristola, M.; Katlama, C.; Viard, J.- P.; Girard, P.- M.; Livrozet, J. M.; Vanhems, P.; Rockstroh, J.; Schmidt, R.; van Lunzen, J.; Degen, O.; Stellbrink, H. J.; Staszewski, S.; Bickel, M.; Kosmidis, J.; Gargalianos, P.; Xylomenos, G.; Perdios, J.; Panos, G.; Filandras, A.; Karabatsaki, E.; Sambatakou, H.; Banhegyi, D.; Mulcahy, F.; Yust, I.; Turner, D.; Burke, M.; Pollack, S.; Hassoun, G.; Maayan, S.; Vella, S.; Esposito, R.; Mazeu, I.; Mussini, C.; Arici, C.; Pristera, R.; Mazzotta, F.; Gabbuti, A.; Vullo, V.; Lichtner, M.; Chirianni, A.; Montesarchio, E.; Gargiulo, M.; Antonucci, G.; Testa, A.; Narciso, P.; Vlassi, C.; Zaccarelli, M.; Lazzarin, A.; Castagna, A.; Gianotti, N.; Galli, M.; Ridolfo, A.; Rozentale, B.; Zeltina, I.; Chaplinskas, S.; Hemmer, R.; Staub, T.; Ormaasen, V.; Maeland, A.; Bruun, J.; Knysz, B.; Gasiorowski, J.; Horban, A.; Bakowska, E.; Grzeszczuk, A.; Flisiak, R.; Boron-Kaczmarska, A.; Pynka, M.; Parczewski, M.; Beniowski, M.; Mularska, E.; Trocha, H.; Jablonowska, E.; Malolepsza, E.; Wojcik, K.; Antunes, F.; Doroana, M.; Caldeira, L.; Mansinho, K.; Maltez, F.; Duiculescu, D.; Rakhmanova, A.; Babes, Victor; Zakharova, N.; Jevtovic, D.; Mokráš, M.; Staneková, D.; Tomazic, J.; González-Lahoz, J.; Soriano, V.; Labarga, P.; Medrano, J.; Moreno, S.; Rodriguez, J. M.; Clotet, B.; Jou, A.; Paredes, R.; Tural, C.; Puig, J.; Bravo, I.; Gatell, J. M.; Miró, J. M.; Gutierrez, M.; Karlsson, A.; Mateo, G.; Flamholc, L.; Ledergerber, B.; Francioli, P.; Cavassini, M.; Hirschel, B.; Boffi, E.; Kravchenko, E.; Furrer, H.; Battegay, M.; Elzi, L.; Chentsova, N.; Frolov, V.; Kutsyna, G.; Servitskiy, S.; Krasnov, M.; Barton, S.; Johnson, A. M.; Mercey, D.; Johnson, M. A.; Murphy, M.; Weber, J.; Scullard, G.; Fisher, M.; Leen, C.; Morfeldt, L.; Thulin, G.; Åkerlund, B.; Koppel, K.; Håkangård, C.; Moroni, M.; Angarano, G.; Antinori, A.; Armignacco, O.; Castelli, F.; Cauda, R.; Di Perri, G.; Iardino, R.; Ippolito, G.; Perno, C. F.; von Schloesser, F.; Viale, P.; Ceccherini-Silberstein, F.; Girardi, E.; Lo Caputo, S.; Puoti, M.; Andreoni, Massimo; Ammassari, Adriana; Antinori, Andrea; Balotta, Claudia; Bonfanti, Paolo; Bonora, Stefano; Borderi, Marco; Capobianchi, M. Rosaria; Castagna, Antonella; Ceccherini-Silberstein, Francesca; Cingolani, Antonella; Cinque, Paola; Cozzi-Lepri, Alessandro; de Luca, Andrea; Di Biagio, Antonio; Girardi, Enrico; Gianotti, Nicola; Gori, Andrea; Guaraldi, Giovanni; Lapadula, Giuseppe; Lichtner, Miriam; Madeddu, Giordano; Maggiolo, Franco; Marchetti, Giulia; Marcotullio, Simone; Monno, Laura; Mussini, Cristina; Puoti, Massimo; Quiros, Eugenia; Rusconi, Stefano; Cicconi, P.; Formenti, T.; Galli, L.; Lorenzini, P.; Giacometti, A.; Costantini, A.; Monno, L.; Santoro, C.; Maggiolo, F.; Suardi, C.; Vanino, E.; Verucchi, G.; Quiros Roldan, E.; Minardi, C.; Quirino, T.; Abeli, C.; Manconi, P. E.; Piano, P.; Vecchiet, J.; Falasca, K.; Sighinolfi, L.; Segala, D.; Cassola, G.; Viscoli, G.; Alessandrini, A.; Piscopo, R.; Mazzarello, G.; Mastroianni, C.; Belvisi, V.; Bonfanti, P.; Caramma, I.; Castelli, A. P.; Rizzardini, G.; Ridolfo, A. L.; Piolini, R.; Salpietro, S.; Carenzi, L.; Moioli, M. C.; Marchetti, G.; Puzzolante, C.; Gori, A.; Onofrio, M.; Lapadula, G.; Abrescia, N.; Guida, M. G.; Baldelli, F.; Francisci, D.; Parruti, G.; Ursini, T.; Magnani, G.; Ursitti, M. A.; Andreoni, M.; Cingolani, A.; d' Avino, A.; Ammassari, A.; Gallo, L.; Nicastri, E.; Acinapura, R.; Capozzi, M.; Libertone, R.; Tebano, G.; Cattelan, A.; Mura, M. S.; Madeddu, G.; Caramello, P.; Orofino, G. C.; Bonora, S.; Sciandra, M.; Pellizzer, G.; Manfrin, V.; Caissotti, C.; Dellamonica, P.; Bernard, E.; Cua, E.; de Salvador- Guillouet, F.; Durant, J.; Ferrando, S.; Mondain-Miton, V.; Naqvi, A.; Perbost, I.; Prouvost-Keller, B.; Pillet, S.; Pugliese, P.; Rahelinirina, V.; Roger, P. M.; Dollet, K.; Aubert, V.; Barth, J.; Bernasconi, E.; Böni, J.; Bucher, H. C.; Burton- Jeangros, C.; Calmy, A.; Egger, M.; Fehr, J.; Fellay, J.; Gorgievski, M.; Günthard, H.; Haerry, D.; Hasse, B.; Hirsch, H. H.; Hösli, I.; Kahlert, C.; Kaiser, L.; Keiser, O.; Klimkait, T.; Kovari, H.; Martinetti, G.; de Tejada, B. Martinez; Metzner, K.; Müller, N.; Nadal, D.; Pantaleo, G.; Rauch, A.; Regenass, A.; Rudin, C.; Schmid, P.; Schultze, D.; Schöni-Affolter, F.; Schüpbach, J.; Speck, R.; Taffé, P.; Tarr, P.; Telenti, A.; Trkola, A.; Vernazza, P.; Yerly, S.

    2014-01-01

    No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90 ml/min/1.73 m2 (using Cockcroft Gault) in the Data

  5. Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons : the D:A:D study

    NARCIS (Netherlands)

    Kamara, David A; Ryom, Lene; Ross, Michael; Kirk, Ole; Reiss, Peter; Morlat, Philippe; Moranne, Olivier; Fux, Christoph A; Mocroft, Amanda; Sabin, Caroline; Lundgren, Jens D; Smith, Colette J; Schölvinck, Elisabeth H.

    2014-01-01

    BACKGROUND: No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90 ml/min/1.73 m2 (using Cockcroft Gault) in the

  6. Development of a definition for Rapid Progression (RP) of renal function in HIV-positive persons: the D:A:D study

    NARCIS (Netherlands)

    Kamara, D.A.; Ryom, L.; Ross, M.; Kirk, O.; Reiss, P.; Morlat, P.; Moranne, O.; Fux, C.A.; Mocroft, A.; Sabin, C.; Lundgren, J.D.; Smith, C.J.; Koopmans, P.P.; Keuter, M.; Ven, A.J.A.M. van der; Hofstede, H.J.M. ter; Dofferhoff, A.S.M.; Warris, A.; Crevel, R. van

    2014-01-01

    BACKGROUND: No consensus exists on how to define abnormally rapid deterioration in renal function (Rapid Progression, RP). We developed an operational definition of RP in HIV-positive persons with baseline estimated glomerular filtration rate (eGFR) >90 ml/min/1.73 m2 (using Cockcroft Gault) in

  7. Factors related to sexual behaviors and sexual education programs for Asian-American adolescents.

    Science.gov (United States)

    Lee, Young-Me; Florez, Elizabeth; Tariman, Joseph; McCarter, Sarah; Riesche, Laren

    2015-08-01

    To understand the influential factors related to sexual behaviors among Asian-American adolescents and to evaluate common factors across successful sexual education programs for this population. Despite a rapid increase in cases of STIs/HIV among Asian-American populations, there remains a need for a comprehensive understanding of the influential factors related to risky sexual behaviors for this population. An integrative literature review was conducted. Peer-reviewed articles and government resources were analyzed. Five influential factors were identified: family-centered cultural values, parental relationship, acculturation, gender roles, and lack of knowledge and information about sex and STIs. Only two sexual educational programs met the inclusion criteria and provided evidence towards effectiveness: Safer Choices and Seattle Social Development Project. The findings of this study indicate an urgent need for culturally sensitive sexual education programs that incorporate the identified influential factors, especially cultural values in order to reduce risky sexual behaviors among Asian-American adolescents. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke

    Directory of Open Access Journals (Sweden)

    Cahours X

    2014-12-01

    Full Text Available Currently, several in vitro tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames Test. These tests are necessary to assess cytotoxicity, genotoxicity, and mutagenicity, but are time consuming. This is essentially due to the preparation and the handling of cells. It is difficult to use these in vitro tests as screening method for product testing and development. For a better assessment of the cytotoxicity of the vapor phase, a rapid cell-free method has been developed. This paper describes a capillary electrophoresis cell-free method, based on the depletion of an anti-oxidant L-gamma-glutamyl-L-cysteinylglycine (GSH, applied to an aliquot of vapor phase phosphate buffered saline (PBS-trapped cigarette smoke (as recommended for in vitro testing. The correlation between this method and the survival/viability test (Neutral Red cytotoxicity is excellent (coefficient of correlation (r = 0.99.

  9. Rapid development of thymic neuroendocrine carcinoma despite transcervical thymectomy in a patient with multiple endocrine neoplasia type 1

    Directory of Open Access Journals (Sweden)

    Dhalapathy Sadacharan

    2013-01-01

    Full Text Available Thymic neuroendocrine (NE tumors are a rare manifestation of multiple endocrine neoplasia syndrome type 1 (MEN-1. They are malignant and aggressive tumors and form a major cause of mortality in MEN-1. Transcervical thymectomy (TCT at the time of parathyroid surgery for primary hyperparathyroidism (PHPT in MEN-1 usually prevents thymic NE tumors. We report a 56-year-old nonsmoker male with sporadic MEN-1 who presented with thymic NE carcinoma developing rapidly within a span of 8 months after subtotal parathyroidectomy and TCT for PHPT. We present a brief review of literature on this rare NE malignancy, focusing on its occurrence despite TCT. This case highlights the fact that thymic NE carcinoma may develop even after TCT in MEN-1. Regular surveillance for these aggressive thymic NE tumors is mandatory even after TCT in MEN-1 setting.

  10. Black Carbon and Kerosene Lighting: An Opportunity for Rapid Action on Climate Change and Clean Energy for Development

    Energy Technology Data Exchange (ETDEWEB)

    Jacobson, Arne [Humboldt State Univ., MN (United States). Schatz Energy Research Center; Bond, Tami C. [Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Civil and Environmental Engineering; Lam, Nicholoas L. [Univ. of California, Berkeley, CA (United States). Dept. of Environmental Health Sciences; Hultman, Nathan [The Brookings Institution, Washington, DC (United States)

    2013-04-15

    Replacing inefficient kerosene lighting with electric lighting or other clean alternatives can rapidly achieve development and energy access goals, save money and reduce climate warming. Many of the 250 million households that lack reliable access to electricity rely on inefficient and dangerous simple wick lamps and other kerosene-fueled light sources, using 4 to 25 billion liters of kerosene annually to meet basic lighting needs. Kerosene costs can be a significant household expense and subsidies are expensive. New information on kerosene lamp emissions reveals that their climate impacts are substantial. Eliminating current annual black carbon emissions would provide a climate benefit equivalent to 5 gigatons of carbon dioxide reductions over the next 20 years. Robust and low-cost technologies for supplanting simple wick and other kerosene-fueled lamps exist and are easily distributed and scalable. Improving household lighting offers a low-cost opportunity to improve development, cool the climate and reduce costs.

  11. Asian and Hispanic Americans' cancer fatalism and colon cancer screening.

    Science.gov (United States)

    Jun, Jungmi; Oh, Kyeung Mi

    2013-03-01

    To explore fatalistic attributions of colon cancer development among Asian and Hispanic Americans in comparison with non-Hispanic whites; also to examine the impacts of fatalism on adherence to the colon cancer screening guideline. For the analysis, the 2005 Health Information National Trends Survey data were employed. Both Asian and Hispanic Americans were more likely to make fatalistic attribution and were less likely to follow the guideline than whites. Particularly for Asians, fatalism was a significant predictor for not adhering to the guideline. These findings emphasize the need for cultural interventions to disrupt fatalistic attitudes towards colon cancer preventions.

  12. Development of a rapid agglutination latex test for diagnosis of enteropathogenic and enterohemorrhagic Escherichia coli infection in developing world: defining the biomarker, antibody and method.

    Directory of Open Access Journals (Sweden)

    Letícia B Rocha

    2014-09-01

    Full Text Available Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC/EHEC are human intestinal pathogens responsible for diarrhea in both developing and industrialized countries. In research laboratories, EPEC and EHEC are defined on the basis of their pathogenic features; nevertheless, their identification in routine laboratories is expensive and laborious. Therefore, the aim of the present work was to develop a rapid and simple assay for EPEC/EHEC detection. Accordingly, the EPEC/EHEC-secreted proteins EspA and EspB were chosen as target antigens.First, we investigated the ideal conditions for EspA/EspB production/secretion by ELISA in a collection of EPEC/EHEC strains after cultivating bacterial isolates in Dulbecco's modified Eagle's medium (DMEM or DMEM containing 1% tryptone or HEp-2 cells-preconditioned DMEM, employing either anti-EspA/anti-EspB polyclonal or monoclonal antibodies developed and characterized herein. Subsequently, a rapid agglutination latex test (RALT was developed and tested with the same collection of bacterial isolates.EspB was defined as a biomarker and its corresponding monoclonal antibody as the tool for EPEC/EHEC diagnosis; the production of EspB was better in DMEM medium. RALT assay has the sensitivity and specificity required for high-impact diagnosis of neglected diseases in the developing world.RALT assay described herein can be considered an alternative assay for diarrhea diagnosis in low-income countries since it achieved 97% sensitivity, 98% specificity and 97% efficiency.

  13. Southeast Asian Refugee Parent Survey.

    Science.gov (United States)

    Blakely, Mary M.

    This paper summarizes the findings of a descriptive research project conducted among Southeast Asian parents in an Oregon school district, and discusses the issue of fieldwork methodology among refugee populations. The district studied had a student population of 18,000 (kindergarten through grade 12), with Southeast Asian refugees accounting for…

  14. Additional Resources on Asian Americans.

    Science.gov (United States)

    Kodama, Corinne Maekawa; Lee, Sunny; Liang, Christopher T. H.; Alvarez, Alvin N.; McEwen, Marylu K.

    2002-01-01

    The authors identify Asian American associations and organizations, academic journals, periodicals, and media resources. Selected annotated resources on Asian American activism and politics, counseling and psychology, educational issues, gender and sexual orientation, history, policy reports, and racial and ethnic identity are also included.…

  15. The Asian Newspaper's Reluctant Revolution.

    Science.gov (United States)

    Lent, John A., Ed.

    This book is composed of 19 articles written by both Asian and American scholars on the history and present conditions of newspapers in 15 Asian nations: China, Hong Kong, Japan, Korea, Taiwan, Australia, Burma, Indonesia, Malaysia and Singapore, the Philippines, Thailand, South Vietnam, Ceylon, India, and Pakistan. Two overviews of the Asian…

  16. Prevalence of organic and inorganic contaminants within a rapidly developing catchment

    Science.gov (United States)

    Njumbe, E. S.; Curtis, C. D.; Cooke, D. A.; Polya, D. A.; Wogelius, R. A.; Hughes, C.

    2003-04-01

    Industrialization rates in many developing countries typically outpace investment in water supply, sewage treatment and other waste water facilities. This is futher compounded by the absence of stringent land-use and waste disposal policies. The consequence of this has been contamination of land, surface water, and groundwater in such areas. Efforts to control and remediate these types of systems will rely on a thorough understanding of contaminant levels and mobility. Reliable data, however, is usually not available. Therefore this study was designed to acquire baseline data from a representative developing urban area in tropical west Africa. 43 water and 20 sediment/soil samples from streams, hand-dug wells, springs and deep boreholes within the city and surrounding areas of Douala in Cameroon were characterised. Analyses were aimed at obtaining information on the type and quantity of organic and inorganic contaminants present, and linking them to specific point and non-point sources. Results from gas chromatography (GC/FID) and gas chromatography/mass spectrometry analyses of total organic extracts (TOE) of water samples have revealed the presence of a wide range of organic compounds including phenols, polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOCs), phthalates, acids and aliphatic derivatives. Concentrations as high as 500 ng ml-1 were detected. These high levels of non-polar compounds measured in drinking water represent a clear health problem. Heavy metal concentrations in bulk alluvial sands and loamy soil have been determined by microwave assisted nitric acid digestion. Concentration ranges (in ppm of dry weight) for the important metals were: Cr, 3.2-84.2 ; Ni, 0.2-57.4 ; Zn, 2.1-92 ; Pb, 0.3-33 ; As, 0.081-9.4 ; Cu, 0.61-17.4 ; and Cd, 0-3.1. Point sources have been identified for several of the organic and inorganic compounds and this spatial information will be integrated with the chemical data to present an overview of

  17. Rapid development of cardiac dysfunction in a canine model of insulin resistance and moderate obesity.

    Science.gov (United States)

    Broussard, Josiane L; Nelson, Michael D; Kolka, Cathryn M; Bediako, Isaac Asare; Paszkiewicz, Rebecca L; Smith, Laura; Szczepaniak, Edward W; Stefanovski, Darko; Szczepaniak, Lidia S; Bergman, Richard N

    2016-01-01

    The worldwide incidence of obesity and diabetes continues to rise at an alarming rate. A major cause of the morbidity and mortality associated with obesity and diabetes is heart disease, yet the mechanisms that lead to cardiovascular complications remain unclear. We performed cardiac MRI to assess left ventricular morphology and function during the development of moderate obesity and insulin resistance in a well-established canine model (n = 26). To assess the influence of dietary fat composition, we randomised animals to a traditional lard diet (rich in saturated and monounsaturated fat; n = 12), a salmon oil diet (rich in polyunsaturated fat; n = 8) or a control diet (n = 6). High-fat feeding with lard increased body weight and fasting insulin and markedly reduced insulin sensitivity. Lard feeding also significantly reduced left ventricular function, evidenced by a worsening of circumferential strain and impairment in left ventricular torsion. High-fat feeding with salmon oil increased body weight; however, salmon oil feeding did not impair insulin sensitivity or cardiac function. These data emphasise the importance of dietary fat composition on both metabolic and cardiac function, and have important implications for the relationship between diet and health.

  18. Development and evaluation of loop-mediated isothermal amplification assay for rapid detection of Capripoxvirus

    Directory of Open Access Journals (Sweden)

    Kanisht Batra

    2015-11-01

    Full Text Available Aim: The present study was undertaken to develop a nucleic acid-based diagnostic assay loop-mediated isothermal amplification assay (LAMP targeting highly conserved genomic regions of Capripoxvirus (CaPVs and its comparative evaluation with real-time polymerase chain reaction (PCR. Material and Methods: Lyophilized vaccine strain of sheeppox virus (SPPV was used for optimization of LAMP assay. The LAMP assay was designed using envelope immunogenic protein (P32 coding gene targeting highly conserved genomic regions of CaPV responsible for causing sheep pox, goat pox, and lumpy skin disease in sheep, goat and cattle respectively. Serial tenfold dilution of SPPV recombinant plasmid DNA was used for a calculating limit of detection. Analytical sensitivity and specificity were performed. Results: The test described is quick (30 min, sensitive and specific for detection of CaPVs. The described assay did not show any cross-reactivity to other related viruses that cause apparently similar clinical signs. It was found to be ten times more sensitive than conventional PCR however, 100 times less sensitive than quantitative PCR (qPCR. LAMP assay results were monitored by color change method using picogreen dye and agarose gel electrophoresis. Conclusion: LAMP assay can be a very good alternative for CaPV detection to other molecular techniques requiring sophisticated equipments.

  19. Development and evaluation of loop-mediated isothermal amplification assay for rapid detection of Capripoxvirus.

    Science.gov (United States)

    Batra, Kanisht; Kumar, Aman; Kumar, Vinay; Nanda, Trilok; Maan, Narender S; Maan, Sushila

    2015-11-01

    The present study was undertaken to develop a nucleic acid-based diagnostic assay loop-mediated isothermal amplification assay (LAMP) targeting highly conserved genomic regions of Capripoxvirus (CaPVs) and its comparative evaluation with real-time polymerase chain reaction (PCR). Lyophilized vaccine strain of sheeppox virus (SPPV) was used for optimization of LAMP assay. The LAMP assay was designed using envelope immunogenic protein (P32) coding gene targeting highly conserved genomic regions of CaPV responsible for causing sheep pox, goat pox, and lumpy skin disease in sheep, goat and cattle respectively. Serial tenfold dilution of SPPV recombinant plasmid DNA was used for a calculating limit of detection. Analytical sensitivity and specificity were performed. The test described is quick (30 min), sensitive and specific for detection of CaPVs. The described assay did not show any cross-reactivity to other related viruses that cause apparently similar clinical signs. It was found to be ten times more sensitive than conventional PCR however, 100 times less sensitive than quantitative PCR (qPCR). LAMP assay results were monitored by color change method using picogreen dye and agarose gel electrophoresis. LAMP assay can be a very good alternative for CaPV detection to other molecular techniques requiring sophisticated equipments.

  20. Development of a lateral flow immunoassay for the rapid diagnosis of invasive candidiasis

    Directory of Open Access Journals (Sweden)

    Zhengxin He

    2016-09-01

    Full Text Available Early and accurate diagnosis of invasive candidiasis (IC is very important. In this study, a lateral flow immunoassay (LFIA was developed to detect antibody against Candida albicans enolase (Eno. Colloidal gold particle labeled mouse anti human IgG (1.0 mg/L was used as the detector reagent. Recombinant enolase (rEno, 1.0 mg/L and goat anti IgG (1.0 mg/L were immobilized in test and control lines, respectively, of a nitrocellulose membrane, acting as the capture reagents. The LFIA was used to detect anti Eno in 38 sera from clinically proven IC patients, as well as in 50 healthy control subjects. Compared with an indirect ELISA designed as a reference test, the specificity and sensitivity of the LFIA were 98.2% and 84.8%, respectively. Excellent agreement between the results obtained by ELISA and the LFIA (kappa = 0.851 was observed in this study. In addition, the agreement between the blood culture results and LFIA test is strong (kappa = 0.658. The data presented in the study indicate that the LFIA test is a suitable tool for the serological surveillance of IC in the field or in poorly equipped laboratories.