Leedu lükkas tagasi teate suundumisest piraadijahile / Evelyn Kaldoja

Index Scriptorium Estoniae

Kaldoja, Evelyn, 1980-

2008-01-01

Leedulaste seas põhjustas hämmingut Euroopa Liidu eesistuja Prantsusmaa kaitseministri Herve Morini teade, et ka Leedu merevägi tahab osaleda piraatlusevastasel operatsioonil Somaalia rannikul. Vt. samas: Kaaperdatud laev

Supramolecular polypseudorotaxane gels for controlled delivery of rAAV vectors in human mesenchymal stem cells for regenerative medicine.

Science.gov (United States)

Rey-Rico, Ana; Babicz, Heiko; Madry, Henning; Concheiro, Angel; Alvarez-Lorenzo, Carmen; Cucchiarini, Magali

2017-10-15

The aim of this work was to investigate, for the first time, the possibility of using supramolecular polypseudorotaxane gels as scaffolds that can durably deliver rAAV vectors for applications in cartilage regeneration. Dispersions of Pluronic ® F68 (PF68) or Tetronic ® 908 (T908) containing either hyaluronic acid (HA) or chondroitin sulfate (CS) were prepared in PBS. Then, alpha-cyclodextrin (αCD) was added to some dispersions to form polypseudorotaxane gels. Polysaccharides and αCD reinforced the viscoelasticity of the gels, which could withstand autoclaving without changes. In vitro release of rAAV vectors and subsequent transduction of human mesenchymal stem cells (hMSCs) by rAAV vectors from the release medium and from gels in direct contact with the cells were investigated. Compared with free vectors, the gels provided higher levels of transgene expression. CS (or HA)/PF68/αCD gels rapidly released rAAV vectors while CS (or HA)/T908/αCD gels provided sustained release probably due to different interactions with the viral vectors. Incorporation of αCD into CS (or HA)/PF68 gels resulted on higher rAAV concentrations and sustained levels of transgene expression over time. HA increased the bioactivity and cytocompatibility of the gels, especially those based on T908. Overall, combining rAAV gene transfer with polypseudorotaxane gels may provide new, promising tools for human tissue engineering and regenerative medicine strategies. Copyright © 2017 Elsevier B.V. All rights reserved.

Uudised : "Eesti väärtorelid" 3. Verdi festival Estonias. Leedu ooper 80 / Tiina Mattisen

Index Scriptorium Estoniae

Mattisen, Tiina

2001-01-01

6. jaan. esitleti Jaani kirikus sarja "Eesti väärtorelid" kolmandat CDd, mis jäädvustab A. Terkmanni 1913.a valminud oreli kõla. G. Verdi 100. surma-aastapäevale pühendatud Verdi festivalist Rahvusooperis Estonia 19.-27. jaan. Leedu Rahvuslik Ooperi- ja Balletiteater tähistas leedu ooperikunsti 80. sünnipäeva 30. dets. galakontserdiga, Estoniat esindas M. Palm

Longevity of rAAV vector and plasmid DNA in blood after intramuscular injection in nonhuman primates: implications for gene doping.

Science.gov (United States)

Ni, W; Le Guiner, C; Gernoux, G; Penaud-Budloo, M; Moullier, P; Snyder, R O

2011-07-01

Legitimate uses of gene transfer technology can benefit from sensitive detection methods to determine vector biodistribution in pre-clinical studies and in human clinical trials, and similar methods can detect illegitimate gene transfer to provide sports-governing bodies with the ability to maintain fairness. Real-time PCR assays were developed to detect a performance-enhancing transgene (erythropoietin, EPO) and backbone sequences in the presence of endogenous cellular sequences. In addition to developing real-time PCR assays, the steps involved in DNA extraction, storage and transport were investigated. By real-time PCR, the vector transgene is distinguishable from the genomic DNA sequence because of the absence of introns, and the vector backbone can be identified by heterologous gene expression control elements. After performance of the assays was optimized, cynomolgus macaques received a single dose by intramuscular (IM) injection of plasmid DNA, a recombinant adeno-associated viral vector serotype 1 (rAAV1) or a rAAV8 vector expressing cynomolgus macaque EPO. Macaques received a high plasmid dose intended to achieve a significant, but not life-threatening, increase in hematocrit. rAAV vectors were used at low doses to achieve a small increase in hematocrit and to determine the limit of sensitivity for detecting rAAV sequences by single-step PCR. DNA extracted from white blood cells (WBCs) was tested to determine whether WBCs can be collaterally transfected by plasmid or transduced by rAAV vectors in this context, and can be used as a surrogate marker for gene doping. We demonstrate that IM injection of a conventional plasmid and rAAV vectors results in the presence of DNA that can be detected at high levels in blood before rapid elimination, and that rAAV genomes can persist for several months in WBCs.

Lithuania 1940 / Herbert Foster Anderson

Index Scriptorium Estoniae

Foster Anderson, Herbert

2004-01-01

Stseenid Leedu ennesõjaaegsest pealinnast Kaunasest briti ärimehe H. Foster Andersoni silme läbi 1940. aastal. Lühikokkuvõte raamatust: Foster Anderson, Herbert. Borderline Russia. London : Cresset press, 1942

Enn Tarvel 75 : psühhograafiline skits / Jaan Undusk

Index Scriptorium Estoniae

Undusk, Jaan, 1958-

2007-01-01

Enn Tarvelist kui Läti Henriku kroonika väljaandjast. Arvutikasutuse algusest eesti agraarajaloo uurimisel 1990ndatel aastatel. Tarveli "Adramaa" erilisusest. Enn Tarvel koostööst Juhan Kahkiga. Tarvelist kui Eesti lähiajaloo uurimise korraldajast

Keila vabrikuala ideevõistluse võitsid noored Leedu arhitektid / Piret Peensoo

Index Scriptorium Estoniae

Peensoo, Piret

2007-01-01

Keila endise riidevabriku kinnistu planeerimise võistluse võitsid Leedu arhitektid Andrius Uogintas, Margarita Podagelyte ja Matas Cirtautas ("Active edge"), teise koha sai PiKaSch Architecture Studio Zoltan Schrammeli juhtimisel Ungarist ("Keila 14") ja kolmanda koha Hannes Koppel ja Sander Aas ("Kegel") arhitektuuribüroost Asum Arhitektid

Leedu paviljon Hannoveri maailmanäitusel EXPO 2000 = Lithuanian Pavilion at EXPO 2000 in Hannover

Index Scriptorium Estoniae

2000-01-01

Leedu paviljoni arhitektuurist. Arhitektid Audrius Bucis, Marina Buciene, Gintaras Kuginis, Valdas Ozarinskis, Aida Ceponite. Konstruktsioonid: Vidas Pilvelis, Vaidas Guogis. 4 ill.: pikilõige, põhiplaan, välis- ja sisevaade

rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ

Directory of Open Access Journals (Sweden)

Henning Madry

2012-01-01

Full Text Available Treatment of chondrosarcoma remains a major challenge in orthopaedic oncology. Gene transfer strategies based on recombinant adenoassociated viral (rAAV vectors may provide powerful tools to develop new, efficient therapeutic options against these tumors. In the present study, we tested the hypothesis that rAAV is adapted for a stable and safe delivery of foreign sequences in human chondrosarcoma tissue by transducing primary human chondrosarcoma cells in vitro and in situ with different reporter genes (E. coli lacZ, firefly luc, Discosoma sp. RFP. The effects of rAAV administration upon cell survival and metabolic activities were also evaluated to monitor possibly detrimental effects of the gene transfer method. Remarkably, we provide evidence that efficient and prolonged expression of transgene sequences via rAAV can be safely achieved in all the systems investigated, demonstrating the potential of the approach of direct application of therapeutic gene vectors as a means to treat chondrosarcoma.

Leedu päritolu kasiinoärimees püsib rikkuselt kolmanda ameeriklasena / Erik Aru

Index Scriptorium Estoniae

Aru, Erik

2006-01-01

Väidetavalt Leedu päritolu kasiinoärimehest Sheldon Adelsonist, tema rivaliteedist Steve Wynniga, nende tegevus Hiina Rahvavabariigi territooriumil Macaos. Lisa: Las Vegas kolib Aasiasse. Tabel : Las Vegas Sands Corporation

Väitlus : vastuseks eelolevale / Enn Küng, Kari Tarkiainen, Ülle Tarkiainen

Index Scriptorium Estoniae

Küng, Enn, 1963-

2015-01-01

Väitlus Enn Küngi ning Kari ja Ülle Tarkiaineni vahel, mille aluseks on 2015. a. Akadeemia 8. numbris ilmunud Enn Küngi arvustis Tarkiainenite raamatule "Provinsen bortom havet. Estlands svenska historia 1561-1710" (Lk. 1491-1499)

Vulgarismid nii ja naa / Enn Sarv

Index Scriptorium Estoniae

Sarv, Enn, 1921-2008

1998-01-01

Meenutus Aleksander Solženitsõni "Üks päev Ivan Denissovitši elus" tõlkimisest (eesti keeles 1963, tõlkijad Lennart Meri ja Enn Sarv). Peeter Sauteri poolt kirjutatud tekstist Raul Meele näituse 'Aborigeenide elu' jaoks (vt. Looming nr. 3)

Iskusstvo kino izmenjajet zhizn / Merit Kask

Index Scriptorium Estoniae

Kask, Merit

2006-01-01

Lõppes Pärnu 20. dokumentaal- ja antropoloogiafilmide festival. Festivali grand prix - "Enne Maale naasmist", režissöör Arunas Matelis : Leedu/Saksamaa, Eesti Rahva Auhinna saaja ühisnimetaja "Inimene otsib õnne" all "Saatana kaevur", režissöörid Richard Ladkan, Kief Davidson : Saksamaa 2004. Lisaks "Laureatõ festivalja"

Pärnu elas kaasa laste võitlusele / Merit Kask

Index Scriptorium Estoniae

Kask, Merit

2006-01-01

Lõppes Pärnu 20. dokumentaal- ja antropoloogiafilmide festival. Festivali grand prix - "Enne Maale naasmist", režissöör Arunas Matelis : Leedu/Saksamaa, Eesti Rahva Auhinna saaja ühisnimetaja "Inimene otsib õnne" all "Saatana kaevur", režissöörid Richard Ladkan, Kief Davidson : Saksamaa 2004. Lisaks "XX Pärnu filmifestivali võitjad"

Karakteriga kodu / Karen Jagodin ; kommenteerinud Enn Rajasaar

Index Scriptorium Estoniae

Jagodin, Karen, 1982-

2009-01-01

2008. aastal valminud 2-korruseline eramu Mähel. Arhitekt Enn Rajasaar. Sisearhitekt Aet Seire. Välisviimistluses on kombineeritud tumedat tellist ja heledat krohvi. Sisekujundust läbivad värvid punane ja must. Osa mööbliesemeid (raamatukapid, söögitoa laud) valmisid sisearhitekti erilahendusena

Baltikumi aktsiaturud tõusevad enne kui reaalmajandus / Paul Lukka

Index Scriptorium Estoniae

Lukka, Paul

2008-01-01

Autori hinnangul on jahtuvas Balti majanduses märgata positiivseid tendentse ning kuna inflatsioon on jõudnud oma kõrgpunkti, võib juba oodata selle langemist. Enamike börsiettevõtete ühtlasest langemisest võivad enim võita pikaajalised investorid. Tabel: Positiivsed näited Leedu börsi ettevõtete tulemustest. Graafik: OMX Baltic Benchmark GI Indeks

La crise européenne. Un regard de géographe

Directory of Open Access Journals (Sweden)

Yann Richard

2012-04-01

Full Text Available L’Union européenne est durement affectée par la crise des dettes souveraines. Cette crise peut être interprétée comme la conséquence de plusieurs faits importants : une absence de leadership européen, des inégalités fortes parfois anciennes entre les performances macroéconomiques des pays membres, des faiblesses structurelles déjà observables avant la crise dans certains pays. Mais le principal facteur de fragilité de l’Union européenne réside dans sa division interne. Tant que la problématique de l’intégration européenne ne sera pas appropriée par les citoyens européens eux-mêmes et tant que certaines politiques ne seront pas davantage coordonnées, l’Union restera sur le fil du rasoir. Cette crise met en lumière l’essoufflement d’un certain modèle d’intégration. Dans cet article, on explore ces problématiques avec un regard géographique.

Leedu poliitika ja kultuuri teine hääl : kolme kõlbelise eluloo visand / Leonidas Donskis ; inglise keelest tõlkinud Kaisa Jõgi

Index Scriptorium Estoniae

Donskis, Leonidas, 1962-

2010-01-01

Leedu liberaalsest rahvuslusest välisleedu õpetlaste: kultuuriloolase Vytautas Kavolise, poliitikateadlase Aleksandras Stromase, kirjandusprofessori ja luuletaja Tomas Venclova elu ja tegevuse kaudu.

Läbimurre: Enn Kunila saadab oma maalikogu Soome näitusele / Andres Eilart ; kommenteerinud Harry Liivrand

Index Scriptorium Estoniae

Eilart, Andres

2010-01-01

Helsingi Taidehallis avatakse oktoobri keskel ülevaatenäitus "Viron värit - 150 vuatta virolaista maalaustaidetta Enn Kunilan kokoelmasta" ("Eesti värvid - 150 aastat eesti maalikunsti Enn Kunila kollektsioonist"). Näituse kuraator Harry Liivrand, kujundaja Olev Subbi. Mitmekeelse kunstialbumi koostajaks on kunstiteadlane Eero Epner

Mis on Eesti hariduse eesmärk? / Enn Vatter

Index Scriptorium Estoniae

Vatter, Enn

2003-01-01

Autori arvates ei suudeta valimiseelsetes vaidlustes kokku leppida eelistusis hariduselus. Autor küsib, mis kooli- ja haridustemaatikas peaeesmärk ning mis on alameesmärgid, mis enne peaeesmärgi saavutamist on vaja kätte saada

La possibilité d'un encadrement juridique des lanceurs d'alerte par l'Union européenne

OpenAIRE

Racho, Tania

2016-01-01

Plusieurs raisons peuvent justifier la nécessité d’une réglementation pour protéger les lanceurs d'alerte au niveau de l'Union européenne. Les alertes médiatisées concernent des dysfonctionnements aux dimensions européennes voire internationales comme par exemple avec les affaires révélées par Snowden ou le Luxleaks. La pertinence d'une action de l'Union européenne est évidente et permettrait alors d'accorder une protection minimale identique sur le territoire des 28 États membres. Néanmoins,...

Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs

Directory of Open Access Journals (Sweden)

Yi Lu

2018-03-01

Full Text Available Corneal neovascularization (NV is the major sight-threatening pathology caused by angiogenic stimuli. Current drugs that directly target pro-angiogenic factors to inhibit or reverse the disease require multiple rounds of administration and have limited efficacies. Here, we identify potential anti-angiogenic corneal microRNAs (miRNAs and demonstrate a framework that employs discovered miRNAs as biotherapies deliverable by recombinant adeno-associated viruses (rAAVs. By querying differentially expressed miRNAs in neovascularized mouse corneas induced by alkali burn, we have revealed 39 miRNAs that are predicted to target more than 5,500 differentially expressed corneal mRNAs. Among these, we selected miR-204 and assessed its efficacy and therapeutic benefit for treating injured corneas. Our results show that delivery of miR-204 by rAAV normalizes multiple novel target genes and biological pathways to attenuate vascularization of injured mouse cornea. Importantly, this gene therapy treatment alternative is efficacious and safe for mitigating corneal NV. Overall, our work demonstrates the discovery of potential therapeutic miRNAs in corneal disorders and their translation into viable treatment alternatives.

Lakewoodi Eesti Majas New Jersey osariigis... / Enn Lillemets

Index Scriptorium Estoniae

Lillemets, Enn

2004-01-01

9. X tuletati meelde eesti graafikut ja maalijat Eduard Rüga (1903-1997), kelle loomingust oli välja pandud näitus Ameerika erakogudest. Õhtust võtsid osa E. Rüga abikaasa Leida Rüga, tütred Silja Melts ja Kersti Raimondo. Enn Lillemetsa ettekandest. Vaadati Andres Söödi telefilmi "Eduard Rüga" (1993)

La Nuit européenne des chercheurs / European Researchers' Night

CERN Multimedia

CERN. Geneva

2016-01-01

Cliquez ici pour voir l'événement webcast (Ceci n'est pas un événement officiel financé par la Commission européenne.) Le CERN organise pour la 7ème année consécutive La Nuit européenne des chercheurs et propose de découvrir la science sous un autre angle. Cet événement ouvre au grand public des centaines de lieux de science à travers l'Europe. Le CERN propose les activités suivantes au public local : Au premier étage du Globe (entrée devant le Globe), projections de documentaires scientifiques primés et de court-métrages en VO sous-titrés en français. Voir programme du Globe. Visites guidées d...

Chemie Linz AG Enns - Austria

Directory of Open Access Journals (Sweden)

Editorial, Equipo

1978-11-01

Full Text Available This factory building in Enns has been erected as an enlargement of the Linz facilities that had become too small. It contains an acrylonitrile processing plant and the whole range of accessory installations required for its operation, both from the technical side, such as equipment, etc., and from the personnel facilities side. Outstanding in this project is the elaborate infrastructure, such as the terracing and filling of 40 Ha of land to evercome the risks from the cyclic sweiling of the Danube, or the water supply, treatment and distribution systems for the various installations. Worth mentioning are also the planning for future expansion, which in successive enlargements and additions will cover 160 Ha and employ up to 5,000-6,000 people.

Este edificio fabril se ha levantado en Enns con objeto de ampliar las ya pequeñas instalaciones de Linz. Consta de una planta para la producción de acrilnitrilo y de toda una serie de instalaciones secundarias necesarias para su funcionamiento, tanto de orden técnico —equipos, medios, etc. — como relativos al personal. En su realización destacan las complejas obras de infraestructura, tales como el terraplenado de las 40 Ha de terreno, a fin de evitar los peligros de las periódicas crecidas del Danubio, o el sistema de toma, depuración y distribución de agua para abastecimiento de las distintas instalaciones. Asimismo, cabe mencionar las previsiones hechas de cara al futuro, con sucesivas ampliaciones que llegarán a ocupar 160 Ha, y emplearán a 5.000 ó 6.000 personas.

Leedu poliitika ja kultuuri teine hääl : kolme kõlbelise eluloo visand / Leonidas Donskis ; [inglise keelest] tõlkinud Kaisa Jõgi

Index Scriptorium Estoniae

Donskis, Leonidas, 1962-

2002-01-01

Leedu kolme õpetlase, kultuuriloolase Vytautas Kavolise, poliitikateadlase Aleksandras Stromase, kirjandusprofessori ja luuletaja Tomas Venclova, elu ja tegevus. Tõlgitud käsikirjast "The second voice of Lithuanian politics and culture: sketches of three moral biographies"

Normes comptables européennes du secteur public (EPSAS)

DEFF Research Database (Denmark)

Andernack, Isabelle; Pontoppidan, Caroline Aggestam

2014-01-01

Les EPSAS, adaptation des IFRS pour le secteur public, devraient fournir à terme des informations plus fiables et transparentes sur la situation et la performance financière des États de l’Union européenne - notamment sur leur niveau d’endettement et leurs engagements - sur la soutenabilité des...

Le syndicalisme, acteur de la démocratisation de l'Union Européenne?

OpenAIRE

Erne, Roland

2002-01-01

Les syndicats pourraient être des agents de la démocratisation de l'Union européenne pour deux raisons : primo, le syndicalisme a souvent joué un rôle substantiel dans les processus de démocratisation nationale. Secundo, les syndicats ont été parmi les premières organisations citoyennes à être impliquées dans le processus d'intégration européenne. Cependant, ces considérations ne signifient pas nécessairement qu'ils joueront un rôle important dans la démocratisation de l'UE....

Tensions at EU-Russia Summit / Andrew Rettman

Index Scriptorium Estoniae

Rettman, Andrew

2009-01-01

Enne Euroopa Liidu-Venemaa tippkohtumist Kaug-Idas ütles Tšehhi president Vaclav Klaus päevalehes Lidove Noviny, et ta ei näe Venemaas ohtu, vaid suurt, tugevat ja ambitsioonikat riiki, millele tuleb rohkem tähelepanu pöörata kui riikidele nagu Eesti ja Leedu. President Toomas Hendrik Ilves väljendas üllatust, et selliste kahetsusväärsete sõnadega - mis jagavad Euroopa riigid olulisteks ja ebaolulisteks - esines just tšehh

Hansa nimemuutus võib viga olla / Iivi Riivits-Arkonsuo

Index Scriptorium Estoniae

Riivits-Arkonsuo, Iivi

2006-01-01

Turu-uuringute AS-i uuringust, milles võrreldi Eesti, Läti ja Leedu elanike suhtumist üksteise toiduainetesse ning seda, kuidas nad ostukäitujana tegutsevad. Autori sõnul võib Baltic Brandi konkursside põhjal väita, et Eesti brändidel läheb Lätis ja Leedus hästi, Hansapank on ka kohaliku brändina hästi vastu võetud, seepärast peaksid uued omanikud tõsiselt mõtlema, enne kui brändi nime välja vahetavad

US Baltic Foundation award winners from Estonia are Arvo Pärt and Enn Kunila

Index Scriptorium Estoniae

2010-01-01

Washingtonis toimunud tänavusel U.S Baltic Foundationi auhinnagalal tunnustati kahte eestlast: helilooja Arvo Pärti auhinnaga Baltimaade kuvandi rikastamise eest (Baltic Image Enhancement Award) ja ettevõtjast kunstikollektsionääri Enn Kunilat Baltimaade filantroopiaauhinnaga

ÉTUDE COMPARATIVE DANS LE SECTEUR DE L’AGRICULTURE DE L’UNION EUROPÉENNE

Directory of Open Access Journals (Sweden)

Constantin CIUTACU

2008-12-01

Full Text Available Le secteur agricole est un pilier central de la construction européenne et il occupe, des lors, une place prépondérante dans les politiques européennes. L’agriculture doit faire face a des défis importants, résultant de l’internationalisation et de la mondialisation des politiques et marchés agricoles. La cohésion sociale et économique tout comme la compétitivité économique donnent une nouvelle dimension européenne aux politiques agricoles communes et il y a lieu de les considérer en rapport avec les politiques en matiere de développement rural ainsi qu’avec les nécessités de développement durable. Un large éventail de problemes affecte actuellement le secteur, par exemple la surpopulation et le dépeuplement agricole, le vieillissement démographique, le niveau et la sécurité des revenus, l’ESB et la fievre aphteuse, les organismes génétiquement modifiés, la biodiversité, le changement climatique, la protection de l’environnement, l’opposition entre agriculture organique et agriculture productive, le bien-etre des animaux, l’érosion du sol et la gestion de l’eau. Les actuels problemes structurels ont des conséquences considérables, particulierement dans les nouveaux et les futurs États membres de l’UE.

ENN-ICS - quality assurance and online evaluation of a multilingual learning management system for sleep medicine

Directory of Open Access Journals (Sweden)

Knobl, Brigitte

2006-11-01

Full Text Available With the development and implementation of a multilingual interactive communication system the e-health project ENN-ICS, funded by the European Union, aims at the improvement of health care in Europe. The field of application is sleep physiology and sleep medicine. ENN-ICS Centre offers direct access to medical information for multiple users, i.e. health authorities, health care professionals, patients and citizen. Using XML technologies the new web based network integrates advanced e-learning and e-publishing methods for the training of healthcare professionals. Editorial and distributive processes are supported by customized central editorial, content management and learning management systems (CMS, LMS. ENN-ICS e-health services are evaluated by selected user groups in North, Middle and Southern Europe using reliable and scientifically accepted validation instruments. The compliance with essential quality requirements and criteria is tested and verified by using adapted online questionnaires based on the DISCERN questionnaire for evaluating patient information, on the HON principles for evaluating health-related websites and on the GMDS catalogue of quality criteria for electronic publications in medicine. The system architecture and its exemplary applications can be used as a model for future e-health services dealing with neurological and other medical topics.

La culture musicale caribéenne de New York - 14

OpenAIRE

Audebert , Cédric

2012-01-01

Le bassin caribéen est un espace de production musicale foisonnante (reggae, salsa, konpa, meringue, zouk, calypso, etc.) au rayonnement international. Les musiques puisent leur énergie principalement dans l'héritage de la plantation et des quartiers populaires urbains des îles d'origine, mais aussi dans le cosmopolitisme des métropoles d'immigration. La mondialisation des musiques caribéennes s'appuie sur les puissants réseaux nord-américains de production et de diffusion, dont les communaut...

Effective genetic modification and differentiation of hMSCs upon controlled release of rAAV vectors using alginate/poloxamer composite systems.

Science.gov (United States)

Díaz-Rodríguez, P; Rey-Rico, A; Madry, H; Landin, M; Cucchiarini, M

2015-12-30

Viral vectors are common tools in gene therapy to deliver foreign therapeutic sequences in a specific target population via their natural cellular entry mechanisms. Incorporating such vectors in implantable systems may provide strong alternatives to conventional gene transfer procedures. The goal of the present study was to generate different hydrogel structures based on alginate (AlgPH155) and poloxamer PF127 as new systems to encapsulate and release recombinant adeno-associated viral (rAAV) vectors. Inclusion of rAAV in such polymeric capsules revealed an influence of the hydrogel composition and crosslinking temperature upon the vector release profiles, with alginate (AlgPH155) structures showing the fastest release profiles early on while over time vector release was more effective from AlgPH155+PF127 [H] capsules crosslinked at a high temperature (50°C). Systems prepared at room temperature (AlgPH155+PF127 [C]) allowed instead to achieve a more controlled release profile. When tested for their ability to target human mesenchymal stem cells, the different systems led to high transduction efficiencies over time and to gene expression levels in the range of those achieved upon direct vector application, especially when using AlgPH155+PF127 [H]. No detrimental effects were reported on either cell viability or on the potential for chondrogenic differentiation. Inclusion of PF127 in the capsules was also capable of delaying undesirable hypertrophic cell differentiation. These findings are of promising value for the further development of viral vector controlled release strategies. Copyright © 2015 Elsevier B.V. All rights reserved.

Raimond Valgre noorem vend - kes ta oli? : Kuno-Enn Valgre (30.05.1918-02.03.1987) / Valdur Ohmann

Index Scriptorium Estoniae

Ohmann, Valdur, 1958-

2011-01-01

Vastukäivad andmed ärgitasid uurima, kes õigupoolest oli Valgre noorem vend. Kõige ehedamalt annab Enn Valgre sõjaaegse käekäigu edasi ülekuulamisprotokoll 11. novembrist 1944. Ülekuulamisprotokolli tekst

Remodelling of human osteoarthritic cartilage by FGF-2, alone or combined with Sox9 via rAAV gene transfer.

Science.gov (United States)

Cucchiarini, Magali; Terwilliger, Ernest F; Kohn, Dieter; Madry, Henning

2009-08-01

Compensating for the loss of extracellular cartilage matrix, as well as counteracting the alterations of the chondrocyte phenotype in osteoarthritis are of key importance to develop effective therapeutic strategies against this disorder. In the present study, we analysed the benefits of applying a potent gene combination to remodel human osteoarthritic (OA) cartilage. We employed the promising recombinant adeno-associated virus (rAAV) vector to deliver the mitogenic fibroblast growth factor 2 (FGF-2) factor, alone or simultaneously with the transcription factor Sox9 as a key activator of matrix synthesis, to human normal and OA articular chondrocytes. We evaluated the effects of single (FGF-2) or combined (FGF-2/SOX9) transgene expression upon the regenerative activities of chondrocytes in three dimensional cultures in vitro and in cartilage explants in situ. Single overexpression of FGF-2 enhanced the survival and proliferation of both normal and OA chondrocytes, without stimulating the matrix synthetic processes in the increased pools of cells. The mitogenic properties of FGF-2 were maintained when SOX9 was co-overexpressed and concomitant with an increase in the production of proteoglycans and type-II collagen, suggesting that the transcription factor was capable of counterbalancing the effects of FGF-2 on matrix accumulation. Also important, expression of type-X collagen, a marker of hypertrophy strongly decreased following treatment by the candidate vectors. Most remarkably, the levels of activities achieved in co-treated human OA cartilage were similar to or higher than those observed in normal cartilage. The present findings show that combined expression of candidate factors in OA cartilage can re-establish key features of normal cartilage and prevent the pathological shift of metabolic homeostasis. These data provide further motivation to develop coupled gene transfer approaches via rAAV for the treatment of human OA.

Vocabulaire du budget des Communautés européennes

CERN Document Server

European Communities. Luxembourg

1992-01-01

Sujet: Etude terminologique et phraséologique du vocabulaire du règlement financier et d'autres textes relatifs au budget des Communautés européennes. Langues: Français, anglais, allemand, italien, néerlandais, danois, espagnol, portuguais, grec. Présentation: Corpus d'un millier d'entrées en neuf langues, présentées dans l'ordre d'apparition des termes et phrases dans les textes dépouillés, avec indication des sources. Des définitions ou notes précisent certaines notions. Neuf index alphabétiques donnent accès au corpus.

Mis põhjustab/soodustab vägivalda koolis? / Valter Parve, Mikko Lagerspetz, Erki Korp, Enn Toom, Kristiine Vahtramäe

Index Scriptorium Estoniae

2008-01-01

Küsimusele vastavad TÜ Pärnu Kolledži lektor Valter Parve, TLÜ sotsioloogiaprofessor Mikko Lagerspetz, Tallinna laste turvakeskuse juhataja Erki Korp, usaldustelefoni projektijuht Enn Toom ja lastevanemate liidu koolitusjuht Kristiine Vahtramäe

La Turquie et l’Union européenne

Directory of Open Access Journals (Sweden)

Benoît Montabone

2008-03-01

Full Text Available L’article explore les évolutions possibles des relations entre la Turquie et l’Union européenne à l’horizon 2020. Il présente quatre scénarios géopolitiques : l’ancrage européen (scénario normatif, l’intégration à risque (scénario tendanciel, la diversification des partenaires (scénario alternatif et le renversement stratégique (scénario catastrophe. Dans tous les cas, l’accès aux hydrocarbures du Caucase et de la mer Caspienne joue une place centrale.This article investigates the various possible evolutions of the relations between Turkey and the European Union on the horizon 2020. Four resulting geopolitical scenarios are introduced: European anchoring (the normative scenario, Integration at risk (the trend line scenario, Diversification of partners (the alternate scenario, and at last Strategic overthrow (the nightmare one. In every case, the access to the hydrocarbons of Caucasus and Caspian Sea plays a key role.

Kjøring med mistanke om påvirkning av andre rusmidler enn alkohol

Directory of Open Access Journals (Sweden)

Asbjørg S. Christophersen

2009-10-01

Full Text Available  SAMMENDRAGStatens rettstoksikologiske institutt har i løpet av de siste år registrert en stor økning av prøver fra bilføreremistenkt for kjøring under påvirkning av andre rusmidler enn alkohol. Antall positive saker økte 42% fra1994 til 1995. De hyppigste påviste stoffer er tetrahydrocannabinol, amfetamin, benzodiazepiner og opiater(morfin/kodein. Bruk av tyngre narkotiske stoffer har blitt mer vanlig i trafikksammenheng og antall positivetilfeller har økt betydelig mer enn totalt antall saker. Fra 1994 til 1995 økte antall positive amfetaminprøvermed 75% (fra 533 til 937 og antall positive prøver som følge av heroininntak økte med 87% (fra 92 til 172.Bruk av flere stoffer samtidig er vanlig, over 60 % av sakene i 1995 var positive på mer enn ett stoff, alkoholikke medregnet. De fleste tilfeller var representert av menn i alderen 25-32 år (37%. Kvinner var representerti ca. 11% av sakene.Christophersen AS, Skurtveit S, Mørland J. Drivers suspected to drive under the influence of drugs otherthan alcohol. Nor J Epidemiol 1996; 6 (1: 45-48.ENGLISH SUMMARYThe National Institute of Forensic Toxicology has registered a large increase in the number of samples fromdrivers suspected to drive under influence of drugs other than alcohol. The number of drug positive casesincreased 42% from 1994 to 1995. The most frequent drugs detected are tetrahydrocannabinol, amphetamine,benzodiazepines and opiates (morphine/codeine. The frequency of hard narcotic drug use has increased morethan the total number of cases. From 1994 to 1995, the number of amphetamine positive samples increased75% (from 533 to 937 and the number of samples positive due to heroin use (detection of the metabolite 6-monoacetylmorphine in urine increased 87%. Multi-drug use is common and more than one drug wasdetected in more than 60% of the cases from 1995, alcohol not included. Most of the cases were representedby men 25-32 years old (37%. Women were represented in about

Editorial: Alimentation méditerranéenne et santé [Mediterranean diet and health

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Malika BOUCHENAK

2016-06-01

Full Text Available Ce numéro de Nutrition & Santé est un retour sur le 2 Congrès International de la Société Algérienne de Nutrition qui s'est tenu du 13 au 15 octobre 2015, à Alger, et dont la thématique principale portait sur l'Alimentation méditerranéenne et la santé. L'alimentation méditerranéenne représente un véritable trésor culturel et culinaire, prônant la consommation de certains aliments, comme les fruits, les légumes, les légumineuses, les grains entiers, les noix, le poisson. De plus, l'un de ses piliers repose sur la consommation de l'huile d'olive, dont les avantages sont nombreux. A cet effet, l'OMS et la FAO ont reconnu le régime méditerranéen comme un modèle d'alimentation de qualité, sain et durable. Par ailleurs, les régimes méditerranéens traditionnels sont nutritionnellement appropriés, avec des teneurs variées en graisses totales, faibles en acides gras saturés et très faibles en acides gras Trans, et riches en fibres et en vitamines et flavonoïdes antioxydants.

Smart and Controllable rAAV Gene Delivery Carriers in Progenitor Cells for Human Musculoskeletal Regenerative Medicine with a Focus on the Articular Cartilage.

Science.gov (United States)

Rey-Rico, Ana; Cucchiarini, Magali

2017-01-01

Cell therapy using mesenchymal stem cells (MSCs) is a powerful tool for the treatment of various diseases and injuries. Still, important limitations including the large amounts of cells required for application in vivo and the age-related decline in lifespan, proliferation, and potency may hinder the use of MSCs in patients. In this regard, gene therapy may offer strong approaches to optimize the use of MSCs for regenerative medicine. Diverse nonviral and viral gene vehicles have been manipulated to genetically modify MSCs, among which the highly effective and relatively safe recombinant adeno-associated viral (rAAV) vectors that emerged as the preferred gene delivery system to treat human disorders. Yet, clinical adaptation of such gene vehicles may be limited by several hurdles, including the possibility of dissemination to nontarget sites and the presence of immune and toxic responses in the host organism that may impair their therapeutic actions. The use of smart biomaterials acting as interfaces to enhance the temporal and spatial presentation of therapeutic agents in the target place and/or acting as scaffolding for MSC growth is an innovative, valuable approach to overcome these shortcomings that else restrain the efficacy of such potent cell populations. Here, we provide an overview on the most recent tissue engineering approaches based on the use of biomaterials acting as vehicles for rAAV vectors to target MSCs directly in the recipient (in vivo strategy) or as supportive matrices for rAAV-modified MSCs for indirect cell reimplantation (ex vivo strategy) as means to activate the reparative processes in tissues of the musculoskeletal system. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Rahvusvaheline lõimumist ergutav seminar ehk ребята, давайте жит дружно! / Ülly Enn

Index Scriptorium Estoniae

Enn, Ülly

2008-01-01

12.-16. oktoobril korraldas Sihtasutuse Archimedes Euroopa Noored Eesti büroo Ida-Virumaal rahvusvahelise seminari "Intercultural dialogue withhout subtitles", kus osalesid Eesti, Läti, Leedu, Saksamaa ja Soome noorsootöötajad

Application of ENN-1 for Fault Diagnosis of Wind Power Systems

Directory of Open Access Journals (Sweden)

Meng-Hui Wang

2012-01-01

Full Text Available Maintaining a wind turbine and ensuring secure is not easy because of long-term exposure to the environment and high installation locations. Wind turbines need fully functional condition-monitoring and fault diagnosis systems that prevent accidents and reduce maintenance costs. This paper presents a simulator design for fault diagnosis of wind power systems and further proposes some fault diagnosis technologies such as signal analysis, feature selecting, and diagnosis methods. First, this paper uses a wind power simulator to produce fault conditions and features from the monitoring sensors. Then an extension neural network type-1- (ENN-1- based method is proposed to develop the core of the fault diagnosis system. The proposed system will benefit the development of real fault diagnosis systems with testing models that demonstrate satisfactory results.

La faille Nord-Pyrénéenne dans l’Ariège et la Haute-Garonne

NARCIS (Netherlands)

Sitter, de L.U.

1953-01-01

Un des plus grands phénomènes structuraux des Pyrénées est sans doute la faille Nord-Pyrénéenne qui longe la zone axiale depuis la Méditerranée jusqu'à la côte atlantique. Elle est caractérisée par plusieurs particularités exceptionelles, dont le métamorphisme des terrains jurassiques et crétacés

Puente sobre el río Enns, Austria

Directory of Open Access Journals (Sweden)

Polensky & Zöllner, Constructora

1965-09-01

Full Text Available A road bridge has been recently opened over the Enns river at Steyr (Austria. The purpose is to improve traffic and avoid the bottlenecks that often took place. The bridge is supplemented by three other works of lesser importance, namely two bridges and a tunnel for pedestrians. The bridge structure supporting the decks is cellular, box type. It has three compartments, and four vertical partitions, whose thickness varies from 6 m over the intermediate piles to 2.30 m at the crown. There are three spans, of 47, 101 and 70 m length. The bridge intrados forms a smooth curve. Two overhangs extend from the central structure, and provide sidewalks for pedestrians: the deck itself having a width of 17 m. The structure was made in successive overhangs, in 3.50 m sections. Longitudinal prestressing, by means of PZ-A-100 type cables, was done by applying an initial loading of 120 t. The cables run in tubes. After the concrete had reached a suitable strength, the cables were stretched. The tubes were then filled with cement mortar, and in this operation air suction equipment was utilised to make sure that no air cavities were left.Se ha construido y puesto en servicio un puente para carretera sobre el río Enns en Steyr (Austria, con objeto de mejorar la circulación de vehículos y evitar las congestiones que se producían frecuentemente. Complementan al puente otras tres obras de menor importancia, constituidas por dos puentes y un túnel para el paso de peatones. La estructura que soporta el tablero es celular, tipo cajón, de tres células y cuatro almas y tabiques verticales, con canto variable desde 6 m sobre las pilas intermedias a 2,30 m en la clave. El puente se subdivide en tres tramos de 47, 101 y 70 m de luz, respectivamente. El intradós sigue el perfil de una curva suave. Del cuerpo de la estructura salen dos ménsulas que soportan los dos andenes para peatones, quedando el tablero con una anchura total de 17 metros. La estructura se construy

Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes.

Science.gov (United States)

Frisch, Janina; Orth, Patrick; Rey-Rico, Ana; Venkatesan, Jagadeesh Kumar; Schmitt, Gertrud; Madry, Henning; Kohn, Dieter; Cucchiarini, Magali

2017-11-01

Implantation of peripheral blood aspirates induced towards chondrogenic differentiation upon genetic modification in sites of articular cartilage injury may represent a powerful strategy to enhance cartilage repair. Such a single-step approach may be less invasive than procedures based on the use of isolated or concentrated MSCs, simplifying translational protocols in patients. In this study, we provide evidence showing the feasibility of overexpressing the mitogenic and pro-anabolic insulin-like growth factor I (IGF-I) in human peripheral blood aspirates via rAAV-mediated gene transfer, leading to enhanced proliferative and chondrogenic differentiation (proteoglycans, type-II collagen, SOX9) activities in the samples relative to control (reporter rAAV-lacZ) treatment over extended periods of time (at least 21 days, the longest time-point evaluated). Interestingly, IGF-I gene transfer also triggered hypertrophic, osteo- and adipogenic differentiation processes in the aspirates, suggesting that careful regulation of IGF-I expression may be necessary to contain these events in vivo. Still, the current results demonstrate the potential of targeting human peripheral blood aspirates via therapeutic rAAV transduction as a novel, convenient tool to treat articular cartilage injuries. © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Après le Brexit, un diagnostic lucide sur l'avenir de l'Union européenne

OpenAIRE

Kahn, Sylvain

2016-01-01

Faut-il être traumatisé par le Brexit? Le cas des Britanniques est très particulier. Ils sont entrés à reculons dans l'Union européenne en 1973, et ils ont tout de suite commencé par demander des clauses d'exemption. L'UE et les Britanniques, concrètement qu'est-ce que c'est? C'est un pays qui, depuis 43 ans, ne cesse de s'opposer autant que possible à toute avancée fédérale, à davantage d'intégration, et promeut une Europe à la carte. C'est d'ailleurs le seul pays avec le Danemark qui avait ...

Novel strategy for generation and titration of recombinant adeno-associated virus vectors.

Science.gov (United States)

Shiau, Ai-Li; Liu, Pu-Ste; Wu, Chao-Liang

2005-01-01

Recombinant adeno-associated virus (rAAV) vectors have many advantages for gene therapeutic applications compared with other vector systems. Several methods that use plasmids or helper viruses have been reported for the generation of rAAV vectors. Unfortunately, the preparation of large-scale rAAV stocks is labor-intensive. Moreover, the biological titration of rAAV is still difficult, which may limit its preclinical and clinical applications. For this study, we developed a novel strategy to generate and biologically titrate rAAV vectors. A recombinant pseudorabies virus (PrV) with defects in its gD, gE, and thymidine kinase genes was engineered to express the AAV rep and cap genes, yielding PS virus, which served as a packaging and helper virus for the generation of rAAV vectors. PS virus was useful not only for generating high-titer rAAV vectors by cotransfection with an rAAV vector plasmid, but also for amplifying rAAV stocks. Notably, the biological titration of rAAV vectors was also feasible when cells were coinfected with rAAV and PS virus. Based on this strategy, we produced an rAAV that expresses prothymosin alpha (ProT). Expression of the ProT protein in vitro and in vivo mediated by rAAV/ProT gene transfer was detected by immunohistochemistry and a bioassay. Taken together, our results demonstrate that the PrV vector-based system is useful for generating rAAV vectors carrying various transgenes.

Europinė semiotika šiandien | Sémiotique européenne d’aujourd’hui

Directory of Open Access Journals (Sweden)

Kęstutis Nastopka

2004-01-01

Full Text Available Dans le champ sémiotique, deux approches épistémologiques se distinguent : sémiotique européenne et sémiotique américaine. La sémiotique européenne, issue de la théorie du signe linguistique de Ferdinand de Saussure, dans les années 1960 voit se former deux écoles indépendantes : celle de Paris (de Greimas et celle de Tartou-Moscou (de Lotman.La sémiotique dite « standard » de Greimas présente une théorie du discours envisagé comme un tout de signification. En comprenant la signification comme un processus, Greimas décrit les transformations des significations d’après le modèle du discours mythique élaboré par Vladimir Propp. Le monde est traité comme discontinu et composé d’oppositions. Dans les années 1980 l’école greimassienne passe de la sémiotique de faire à celle d’état, de la discontinuité à la continuité, de l’objet au sujet, de l’énoncé à l’énonciation. Dans la sémiotique des passions au centre du programme narratif se trouve une compétence modale du sujet. Ces derniers temps, parmi les héritiers de Greimas, à côté de la sémiotique tensive qui choisit un métalangage savant et des schématisations un peu arides, apparaît une sémiotique esthésique, issue de De l’imperfection de Greimas.Le concept de systèmes modelants secondaires est devenu une sorte d’emblème de l’école de Tartou. Ce terme détermine des structures communicatives différentes, organisées d’après le modèle des signes linguistiques. A la recherche d’un métalangage scientifique, commun aux diverses disciplines humaines, on s’oriente vers la linguistique structurale et la théorie de l’information. Autour de l’année 1980, dans la sémiotique de Lotman la conception du texte commeune manifestation de la langue est changée par celle du texte qui produit sa propre langue. Le texte est considéré non plus comme un systéme autonome, synchronique mais comme un énoncé, inséparable de la m

Disruption of Microtubules Post-Virus Entry Enhances Adeno-Associated Virus Vector Transduction

Science.gov (United States)

Xiao, Ping-Jie; Mitchell, Angela M.; Huang, Lu; Li, Chengwen; Samulski, R. Jude

2016-01-01

Perinuclear retention of viral particles is a poorly understood phenomenon observed during many virus infections. In this study, we investigated whether perinuclear accumulation acts as a barrier to limit recombinant adeno-associated virus (rAAV) transduction. After nocodazole treatment to disrupt microtubules at microtubule-organization center (MT-MTOC) after virus entry, we observed higher rAAV transduction. To elucidate the role of MT-MTOC in rAAV infection and study its underlying mechanisms, we demonstrated that rAAV's perinuclear localization was retained by MT-MTOC with fluorescent analysis, and enhanced rAAV transduction from MT-MTOC disruption was dependent on the rAAV capsid's nuclear import signals. Interestingly, after knocking down RhoA or inhibiting its downstream effectors (ROCK and Actin), MT-MTOC disruption failed to increase rAAV transduction or nuclear entry. These data suggest that enhancement of rAAV transduction is the result of increased trafficking to the nucleus via the RhoA-ROCK-Actin pathway. Ten-fold higher rAAV transduction was also observed by disrupting MT-MTOC in brain, liver, and tumor in vivo. In summary, this study indicates that virus perinuclear accumulation at MT-MTOC is a barrier-limiting parameter for effective rAAV transduction and defines a novel defense mechanism by which host cells restrain viral invasion. PMID:26942476

Unrestricted Hepatocyte Transduction with Adeno-Associated Virus Serotype 8 Vectors in Mice

Science.gov (United States)

Nakai, Hiroyuki; Fuess, Sally; Storm, Theresa A.; Muramatsu, Shin-ichi; Nara, Yuko; Kay, Mark A.

2005-01-01

Recombinant adeno-associated virus (rAAV) vectors can mediate long-term stable transduction in various target tissues. However, with rAAV serotype 2 (rAAV2) vectors, liver transduction is confined to only a small portion of hepatocytes even after administration of extremely high vector doses. In order to investigate whether rAAV vectors of other serotypes exhibit similar restricted liver transduction, we performed a dose-response study by injecting mice with β-galactosidase-expressing rAAV1 and rAAV8 vectors via the portal vein. The rAAV1 vector showed a blunted dose-response similar to that of rAAV2 at high doses, while the rAAV8 vector dose-response remained unchanged at any dose and ultimately could transduce all the hepatocytes at a dose of 7.2 × 1012 vector genomes/mouse without toxicity. This indicates that all hepatocytes have the ability to process incoming single-stranded vector genomes into duplex DNA. A single tail vein injection of the rAAV8 vector was as efficient as portal vein injection at any dose. In addition, intravascular administration of the rAAV8 vector at a high dose transduced all the skeletal muscles throughout the body, including the diaphragm, the entire cardiac muscle, and substantial numbers of cells in the pancreas, smooth muscles, and brain. Thus, rAAV8 is a robust vector for gene transfer to the liver and provides a promising research tool for delivering genes to various target organs. In addition, the rAAV8 vector may offer a potential therapeutic agent for various diseases affecting nonhepatic tissues, but great caution is required for vector spillover and tight control of tissue-specific gene expression. PMID:15596817

Milleks teatrilavale ajalugu? : Katri-Aaslav-Tepandi ja Tõnu Tepandi. "President 1939". Estonia kammersaal, esietendus 18. veebruaril 2008; Enn Vetemaa, Erki Aule ja Merle Karusoo. "Sigma Tau-S705". Eesti Draamateater, esietendus 16. märtsil 2008 /

Index Scriptorium Estoniae

Vseviov, David, 1949-

2009-01-01

Arvustus : Katri-Aaslav-Tepandi ja Tõnu Tepandi. "President 1939". Estonia kammersaal, esietendus 18. veebruaril 2008; Enn Vetemaa, Erki Aule ja Merle Karusoo. "Sigma Tau-S705". Eesti Draamateater, esietendus 16. märtsil 2008

Fédérations multinationales et coordination multi-niveaux. Comparaison des mécanismes de participation régionale à la politique européenne en Allemagne et en Espagne

Directory of Open Access Journals (Sweden)

Emmanuelle Mathieu

2008-06-01

Full Text Available Comment expliquer le recours extensif au bilatéralisme dans les relations intergouvernementales (RIG au sein des États multinationaux ? En étudiant le fédéralisme multinational sous l’angle des jeux d’acteurs, cet article contribue à sa compréhension comme système d’action et enrichit l’étude des RIG par la mise en évidence de nouveaux facteurs structurants. Il montre que l’hétérogénéité caractérisant l’ensemble des entités fédérées favorise le développement de relations individualisées avec l’État fédéral. La démonstration repose sur une comparaison de l’adaptation des RIG espagnoles et allemandes à l’intégration européenne. Les négociations au Conseil de l’Union européenne exigent une position nationale claire et unique, indépendamment du nombre d’acteurs dont les compétences sont concernées dans l’ordre interne. Cette exigence oriente les acteurs nationaux vers un mécanisme multilatéral de participation des entités fédérées à la politique européenne. Alors que le recours au multilatéralisme n’a pas même été questionné en Allemagne, l’Espagne peine à l’intégrer à son système basé sur le bilatéralisme. Le recours au mode d’organisation territorial, notamment au fédéralisme asymétrique et dual, et au multinationalisme permet d’expliquer ces difficultés.

La Cour européenne des droits de l’homme et la défense du mode de vie tsigane : Le choix de l’immobilisme. Observations sous Chapman c. Royaume-Uni, 18 janvier 2001

OpenAIRE

Ringelheim, Julie

2001-01-01

Cet article analyse en profondeur l'arrêt de la Cour européenne des droits de l'homme, Chapman c. Royaume-Uni du 18 janvier 2001 qui porte sur le droit des familles tsiganes à vivre en caravane selon leurs traditions.

The upstream enhancer elements of the G6PC promoter are critical for optimal G6PC expression in murine glycogen storage disease type Ia.

Science.gov (United States)

Lee, Young Mok; Pan, Chi-Jiunn; Koeberl, Dwight D; Mansfield, Brian C; Chou, Janice Y

2013-11-01

Glycogen storage disease type-Ia (GSD-Ia) patients deficient in glucose-6-phosphatase-α (G6Pase-α or G6PC) manifest impaired glucose homeostasis characterized by fasting hypoglycemia, growth retardation, hepatomegaly, nephromegaly, hyperlipidemia, hyperuricemia, and lactic acidemia. Two efficacious recombinant adeno-associated virus pseudotype 2/8 (rAAV8) vectors expressing human G6Pase-α have been independently developed. One is a single-stranded vector containing a 2864-bp of the G6PC promoter/enhancer (rAAV8-GPE) and the other is a double-stranded vector containing a shorter 382-bp minimal G6PC promoter/enhancer (rAAV8-miGPE). To identify the best construct, a direct comparison of the rAAV8-GPE and the rAAV8-miGPE vectors was initiated to determine the best vector to take forward into clinical trials. We show that the rAAV8-GPE vector directed significantly higher levels of hepatic G6Pase-α expression, achieved greater reduction in hepatic glycogen accumulation, and led to a better toleration of fasting in GSD-Ia mice than the rAAV8-miGPE vector. Our results indicated that additional control elements in the rAAV8-GPE vector outweigh the gains from the double-stranded rAAV8-miGPE transduction efficiency, and that the rAAV8-GPE vector is the current choice for clinical translation in human GSD-Ia. © 2013.

Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons

Science.gov (United States)

Saunders, Arpiar; Johnson, Caroline A.; Sabatini, Bernardo L.

2012-01-01

Understanding the organization of the nervous system requires methods for dissecting the contributions of each component cell type to circuit function. One widely used approach combines genetic targeting of Cre recombinase to specific cell populations with infection of recombinant adeno-associated viruses (rAAVs) whose transgene expression is activated by Cre (“Cre-On”). Distinguishing how the Cre-expressing neurons differ functionally from neighboring Cre-negative neurons requires rAAVs that are inactivated by Cre (“Cre-Off”) and can be used in tandem with Cre-On viruses. Here we introduce two rAAV vectors that are inactivated by Cre and carry different fluorophore and optogenetic constructs. We demonstrate single and dual rAAV systems to achieve Cre-On and Cre-Off expression in spatially-intermingled cell populations of the striatum. Using these systems, we uncovered cryptic genomic interactions that occur between multiple Cre-sensitive rAAVs or between Cre-sensitive rAAVs and somatic Cre-conditional alleles and devised methods to avoid these interactions. Our data highlight both important experimental caveats associated with Cre-dependent rAAV use as well as opportunities for the development of improved rAAVs for gene delivery. PMID:22866029

Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and Cap.

Science.gov (United States)

Conway, J E; Zolotukhin, S; Muzyczka, N; Hayward, G S; Byrne, B J

1997-11-01

Recombinant adeno-associated virus (AAV) type 2 (rAAV) vectors have recently been shown to have great utility as gene transfer agents both in vitro and in vivo. One of the problems associated with the use of rAAV vectors has been the difficulty of large-scale vector production. Low-efficiency plasmid transfection of the rAAV vector and complementing AAV type 2 (AAV-2) functions (rep and cap) followed by superinfection with adenovirus has been the standard approach to rAAV production. The objectives of this study were to demonstrate the ability of a recombinant herpes simplex virus type 1 (HSV-1) amplicon expressing AAV-2 Rep and Cap to support replication and packaging of rAAV vectors. HSV-1 amplicon vectors were constructed which contain the AAV-2 rep and cap genes under control of their native promoters (p5, p19, and p40). An HSV-1 amplicon vector, HSV-RC/KOS or HSV-RC/d27, was generated by supplying helper functions with either wild-type HSV-1 (KOS strain) or the ICP27-deleted mutant of HSV-1, d27-1, respectively. Replication of the amplicon stocks is not inhibited by the presence of AAV-2 Rep proteins, which highlights important differences between HSV-1 and adenovirus replication and the mechanism of providing helper function for productive AAV infection. Coinfection of rAAV and HSV-RC/KOS resulted in the replication and amplification of rAAV genomes. Similarly, rescue and replication of rAAV genomes occurred when rAAV vector plasmids were transfected into cells followed by HSV-RC/KOS infection and when two rAAV proviral cell lines were infected with HSV-RC/KOS or HSV-RC/d27. Production of infectious rAAV by rescue from two rAAV proviral cell lines has also been achieved with HSV-RC/KOS and HSV-RC/d27. The particle titer of rAAV produced with HSV-RC/d27 is equal to that achieved by supplying rep and cap by transfection followed by adenovirus superinfection. Importantly, no detectable wild-type AAV-2 is generated with this approach. These results demonstrate

Factors influencing recombinant adeno-associated virus production.

Science.gov (United States)

Salvetti, A; Orève, S; Chadeuf, G; Favre, D; Cherel, Y; Champion-Arnaud, P; David-Ameline, J; Moullier, P

1998-03-20

Recombinant adeno-associated virus (rAAV) is produced by transfecting cells with two constructs: the rAAV vector plasmid and the rep-cap plasmid. After subsequent adenoviral infection, needed for rAAV replication and assembly, the virus is purified from total cell lysates through CsCl gradients. Because this is a long and complex procedure, the precise titration of rAAV stocks, as well as the measure of the level of contamination with adenovirus and rep-positive AAV, are essential to evaluate the transduction efficiency of these vectors in vitro and in vivo. Our vector core is in charge of producing rAAV for outside investigators as part of a national network promoted by the Association Française contre les Myopathies/Généthon. We report here the characterization of 18 large-scale rAAV stocks produced during the past year. Three major improvements were introduced and combined in the rAAV production procedure: (i) the titration and characterization of rAAV stocks using a stable rep-cap HeLa cell line in a modified Replication Center Assay (RCA); (ii) the use of different rep-cap constructs to provide AAV regulatory and structural proteins; (iii) the use of an adenoviral plasmid to provide helper functions needed for rAAV replication and assembly. Our results indicate that: (i) rAAV yields ranged between 10(11) to 5 x 10(12) total particles; (ii) the physical particle to infectious particle (measured by RCA) ratios were consistently below 50 when using a rep-cap plasmid harboring an ITR-deleted AAV genome; the physical particle to transducing particle ratios ranged between 400 and 600; (iii) the use of an adenoviral plasmid instead of an infectious virion did not affect the particles or the infectious particles yields nor the above ratio. Most of large-scale rAAV stocks (7/9) produced using this plasmid were free of detectable infectious adenovirus as determined by RCA; (iv) all the rAAV stocks were contaminated with rep-positive AAV as detected by RCA. In summary

L’indice biologique global normalisé (IBGN) : principes et évolution dans le cadre de la directive cadre européenne sur l’eau

OpenAIRE

Virginie Archaimbault et Bernard Dumont

2010-01-01

Les macro-invertébrés sont un des groupes d’organismes les plus utilisés mondialement dans de nombreuses méthodes de bio-indication en cours d’eau. Nous vous proposons à travers cet article de décrire la méthode française d’évaluation de la qualité du milieu basé sur ce groupe d’organisme (l’IBGN: indice biologique global normalisé) et son évolution dans le contexte de la directive cadre européenne sur l’eau.

A novel and highly efficient production system for recombinant adeno-associated virus vector.

Science.gov (United States)

Wu, Zhijian; Wu, Xiaobing; Cao, Hui; Dong, Xiaoyan; Wang, Hong; Hou, Yunde

2002-02-01

Recombinant adeno-associated virus (rAAV) has proven to be a promising gene delivery vector for human gene therapy. However, its application has been limited by difficulty in obtaining enough quantities of high-titer vector stocks. In this paper, a novel and highly efficient production system for rAAV is described. A recombinant herpes simplex virus type 1 (rHSV-1) designated HSV1-rc/DeltaUL2, which expressed adeno-associated virus type2 (AAV-2) Rep and Cap proteins, was constructed previously. The data confirmed that its functions were to support rAAV replication and packaging, and the generated rAAV was infectious. Meanwhile, an rAAV proviral cell line designated BHK/SG2, which carried the green fluorescent protein (GFP) gene expression cassette, was established by transfecting BHK-21 cells with rAAV vector plasmid pSNAV-2-GFP. Infecting BHK/SG2 with HSV1-rc/DeltaUL2 at an MOI of 0.1 resulted in the optimal yields of rAAV, reaching 250 transducing unit (TU) or 4.28x10(4) particles per cell. Therefore, compared with the conventional transfection method, the yield of rAAV using this "one proviral cell line, one helper virus" strategy was increased by two orders of magnitude. Large-scale production of rAAV can be easily achieved using this strategy and might meet the demands for clinical trials of rAAV-mediated gene therapy.

Une progression sans révolution dans l’appréhension européenne des persécutions religieuses

Directory of Open Access Journals (Sweden)

Nicolas Hervieu

2013-06-01

SOMMAIRE: 1. Introduction - 2. Une issue retentissante: la concrétisation d’une protection conventionnelle contre les persécutions religieuses - 3. Un raisonnement parcellaire: les apories de l’analyse européenne à l’aune de la liberté de religion - 4. Conclusion - 5. Jurisprudence liée - 6. Appendice.  ABSTRACT: By banning deporting of a coptic christian by France to Egypt, where he might suffer persecution, European court of human rights has made an important contribution to the protection of foreigners against measures involving expulsion. The European solution is important because it deals with a complex, but crucial, concept: religious persecution. Unfortunatly, the opportunity offered by this case hase not been fully exploited. Freedom of religion was at the heart of this case, but European court did’nt use it enough.

Process optimization of large-scale production of recombinant adeno-associated vectors using dielectric spectroscopy.

Science.gov (United States)

Negrete, Alejandro; Esteban, Geoffrey; Kotin, Robert M

2007-09-01

A well-characterized manufacturing process for the large-scale production of recombinant adeno-associated vectors (rAAV) for gene therapy applications is required to meet current and future demands for pre-clinical and clinical studies and potential commercialization. Economic considerations argue in favor of suspension culture-based production. Currently, the only feasible method for large-scale rAAV production utilizes baculovirus expression vectors and insect cells in suspension cultures. To maximize yields and achieve reproducibility between batches, online monitoring of various metabolic and physical parameters is useful for characterizing early stages of baculovirus-infected insect cells. In this study, rAAVs were produced at 40-l scale yielding ~1 x 10(15) particles. During the process, dielectric spectroscopy was performed by real time scanning in radio frequencies between 300 kHz and 10 MHz. The corresponding permittivity values were correlated with the rAAV production. Both infected and uninfected reached a maximum value; however, only infected cell cultures permittivity profile reached a second maximum value. This effect was correlated with the optimal harvest time for rAAV production. Analysis of rAAV indicated the harvesting time around 48 h post-infection (hpi), and 72 hpi produced similar quantities of biologically active rAAV. Thus, if operated continuously, the 24-h reduction in the production process of rAAV gives sufficient time for additional 18 runs a year corresponding to an extra production of ~2 x 10(16) particles. As part of large-scale optimization studies, this new finding will facilitate the bioprocessing scale-up of rAAV and other bioproducts.

Lean i Norge : opplever norske bedrifter som er mer lean, større økning i kundetilfredshet enn de som er mindre lean?

OpenAIRE

Fagereng, Hanne Eline; Askevold, Elisabeth Cathrine

2010-01-01

Vi ønsker å måle i hvor stor grad norske bedrifter har implementert lean, og for å måle grad av lean har vi utviklet et rammeverk basert på lean teori. Hovedformålet med lean er å øke kundeverdi med minst mulig ressursbruk, anstrengelse, energi, utstyr, tid, plass, material og kapital (Womack, Roos and Jones 2007). Vi vil utforske om høyere grad av lean fører til mer tilfredse kunder enn de som har lavere grad av lean. Altså om hovedformålet med lean blir oppnådd hvis man implementerer lean i...

Effect and Mechanism of Mitomycin C Combined with Recombinant Adeno-Associated Virus Type II against Glioma

Directory of Open Access Journals (Sweden)

Hong Ma

2013-12-01

Full Text Available The effect of chemotherapy drug Mitomycin C (MMC in combination with recombinant adeno-associated virus II (rAAV2 in cancer therapy was investigated, and the mechanism of MMC affecting rAAV2’s bioactivity was also studied. The combination effect was evaluated by the level of GFP and TNF expression in a human glioma cell line, and the mechanism of MMC effects on rAAV mediated gene expression was investigated by AAV transduction related signal molecules. C57 and BALB/c nude mice were injected with rAAV-EGFP or rAAV-TNF alone, or mixed with MMC, to evaluate the effect of MMC on AAV-mediated gene expression and tumor suppression. MMC was shown to improve the infection activity of rAAV2 both in vitro and in vivo. Enhancement was found to be independent of initial rAAV2 receptor binding stage or subsequent second-strand synthesis of target DNA, but was related to cell cycle retardation followed by blocked genome degradation. In vivo injection of MMC combined with rAAV2 into the tumors of the animals resulted in significant suppression of tumor growth. It was thus demonstrated for the first time that MMC could enhance the expression level of the target gene mediated by rAAV2. The combination of rAAV2 and MMC may be a promising strategy in cancer therapy.

Á contre-courant : les mobilités caribéennes vers l’Éthiopie

Directory of Open Access Journals (Sweden)

Giulia Bonacci

2008-09-01

Full Text Available L’article s’intéresse à la migration des Caribéens vers l’Ethiopie, et notamment celle des Rastafariens qui se définissent comme de « vrais Éthiopiens » et se disent « rapatriés » en Éthiopie. En dépit de leur nombre limité, ils n’en sont pas moins importants car ils illustrent la persistance et les transformations des imaginaires et idéologies liant les Noirs du monde à l’Éthiopie ainsi qu’un pan méconnu des mobilités caribéennes qui, se dirigeant vers l’Afrique, se découvrent complètement à contre-courant.The article deals with Caribbean migration towards Ethiopia, and especially the one of Rastafarians who define themselves as ‘true Ethiopians’ and ‘repatriates’ in Ethiopia. Despite their limited number, their experience is nonetheless important as it reveals the persistence and change in imaginaries and ideologies linking black all over the world to Ethiopia as well as unknown Caribbean mobility towards Africa, a mobility which is driven against the current.

High-titer recombinant adeno-associated virus production utilizing a recombinant herpes simplex virus type I vector expressing AAV-2 Rep and Cap.

Science.gov (United States)

Conway, J E; Rhys, C M; Zolotukhin, I; Zolotukhin, S; Muzyczka, N; Hayward, G S; Byrne, B J

1999-06-01

Recombinant adeno-associated virus type 2 (rAAV) vectors have recently been used to achieve long-term, high level transduction in vivo. Further development of rAAV vectors for clinical use requires significant technological improvements in large-scale vector production. In order to facilitate the production of rAAV vectors, a recombinant herpes simplex virus type I vector (rHSV-1) which does not produce ICP27, has been engineered to express the AAV-2 rep and cap genes. The optimal dose of this vector, d27.1-rc, for AAV production has been determined and results in a yield of 380 expression units (EU) of AAV-GFP produced from 293 cells following transfection with AAV-GFP plasmid DNA. In addition, d27.1-rc was also efficient at producing rAAV from cell lines that have an integrated AAV-GFP provirus. Up to 480 EU/cell of AAV-GFP could be produced from the cell line GFP-92, a proviral, 293 derived cell line. Effective amplification of rAAV vectors introduced into 293 cells by infection was also demonstrated. Passage of rAAV with d27. 1-rc results in up to 200-fold amplification of AAV-GFP with each passage after coinfection of the vectors. Efficient, large-scale production (>109 cells) of AAV-GFP from a proviral cell line was also achieved and these stocks were free of replication-competent AAV. The described rHSV-1 vector provides a novel, simple and flexible way to introduce the AAV-2 rep and cap genes and helper virus functions required to produce high-titer rAAV preparations from any rAAV proviral construct. The efficiency and potential for scalable delivery of d27.1-rc to producer cell cultures should facilitate the production of sufficient quantities of rAAV vectors for clinical application.

"Children are faced with two alternatives, either to watch TV in English or to watch Russian TV"

Index Scriptorium Estoniae

2006-01-01

Vajadusest muuta Leedu televisiooni seadust, et Leedu emigrandid saaksid ka Iirimaal jm vaadata kodumaiseid televisiooniprogramme. Kommenteerib Leedu Raadio ja Televisiooni direktor Kestutis Petrauskis

Visitor from a parallel world / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2009-01-01

Valgevene president Aleksandr Lukashenko kohtus Vilniuses Leedu presidendi Dalia Grybauskaitega . Valgevene opositsioon kohtus Leedu peaministri Andrius Kubiliuse ja Leedu välisministri Vygaudas Usackasega

Cre Activated and Inactivated Recombinant Adeno-Associated Viral Vectors for Neuronal Anatomical Tracing or Activity Manipulation.

Science.gov (United States)

Saunders, Arpiar; Sabatini, Bernardo L

2015-07-01

Recombinant adeno-associated viruses (rAAVs) transcriptionally activated by Cre recombinase (Cre-On) are powerful tools for determining the anatomy and function of genetically defined neuronal types in transgenic Cre driver mice. Here we describe how rAAVs transcriptionally inactivated by Cre (Cre-Off) can be used in conjunction with Cre-On rAAVs or genomic Cre-reporter alleles to study brain circuits. Intracranial injection of Cre-On/Cre-Off rAAVs into spatially intermingled Cre(+) and Cre(-) neurons allows these populations to be differentially labeled or manipulated within individual animals. This comparison helps define the unique properties of Cre(+) neurons, highlighting the specialized role they play in their constituent brain circuits. This protocol touches on the conceptual and experimental background of Cre-Off rAAV systems, including caveats and methods of validation. Copyright © 2015 John Wiley & Sons, Inc.

Lithuanian diplomacy in 2011 / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

16. detsembril esitas Leedu välisministeerium 2011. aasta Leedu diplomaatilise teenistuse raporti, milles kajastati Leedu välispoliitika trende ja tutvustati diplomaatilisi saavutusi ning prioriteete

MTV wins first hearing against Catholic Church over "Popetown" / Arturas Racas

Index Scriptorium Estoniae

Racas, Arturas

2007-01-01

Vilniuse Ringkonnakohtu lahendist Leedu Katoliku Kiriku ja MTV Leedu vahel. Põhjuseks skandaalse multifilmi "Popetown'i" näitamine Leedu televisioonis, milles kujutati solvavalt katoliku kiriku telgitaguseid

Algirdas Brazauskas : Ma ei häbene rahvale oma liini näidata / Algirdas Brazauskas ; interv. Sirje Pärismaa

Index Scriptorium Estoniae

Brazauskas, Algirdas, 1932-2010

2001-01-01

Taasiseseisvunud Leedu esimene president oma ametiaja suurimatest saavutustest, oma edu saladusest, muutustest Leedu ühiskonnas, Balti riikide ühtsusest, Leedu-Vene suhetest, kokkupuudetest Eestiga jm. Eluloolisi andmeid

Enough room for Williams and IMF? / Paul Beckman

Index Scriptorium Estoniae

Beckman, Paul

1999-01-01

Lõppesid Leedu ja USA energeetikakompanii Williams International läbirääkimised Leedu naftakompleksis osaluse omandamise asjus. IMF uurib Leedu majanduslikku arengut, mida tehing Williamsiga komplitseerib

Un inventaire phytosociologique des dunes à la côte méditerranéenne française entre carnon et le grau du roi (département de l’hérault)

NARCIS (Netherlands)

Hekking, W.H.A.

1959-01-01

Dans la région des dunes et des bas-fonds située à une distance de 3.6 à 5.3 kilomètres de Carnon au Grau du Roi, entre l’Etang de Mauguio et la Mer méditerranéenne et dans le département de l’Hérault on peut constater deux suites progressives des associations phytosociologiques : A) LA VÉGÉ TATION

The importance of a lasting impression / Linas Linkevicius ; interv. Steven Paulikas

Index Scriptorium Estoniae

Linkevicius, Linas

2003-01-01

Leedu kaitseminister Linas Linkevicius räägib rahvusvahelistes rahuvalveoperatsioonides osalemise tähtsusest, Leedu - USA tihedast koostööst ning käimasoleva presidendiskandaali mõjust Leedu rahvusvahelisele mainele

Empty virions in AAV8 vector preparations reduce transduction efficiency and may cause total viral particle dose-limiting side effects

Directory of Open Access Journals (Sweden)

Kai Gao

2014-01-01

Full Text Available Empty virions are inadvertent by-products of recombinant adeno-associated virus (rAAV packaging process, resulting in vector lots with mixtures of full and empty virions at variable ratios. Impact of empty virions on the efficiency and side effects of rAAV transduction has not been well characterized. Here, we generated partially and completely empty AAV8 virions, fully packaged rAAV8 lots, and mixtures of empty and fully packaged virions with variable ratios of empty virions. The aforementioned dosing formulations of rAAV8 expressing either cellular (EGFP (enhanced green fluorescent protein or nuclear-targeted (n LacZ or secreted (human α1-antitrypsin (hA1AT reporter genes were intravenously injected into two different mouse strains, followed by analyses of transgene expressions and serum alanine aminotransferase (ALT levels at different time points. We found that addition of empty particles to the fixed doses of rAAV8 preparations repressed liver transduction up to 64% (serum hA1AT and 44% (nLacZ in C57BL/6 mice, respectively. The similar trend in inhibiting EGFP expression together with concurrent elevations of serum ALT levels were observed in the BALB/c mice, indicating that empty particles may also exacerbate side effects of rAAV8 EGFP transduction. Our results suggest that removal of empty particles from rAAV preparations may improve efficacy and safety of AAV in clinical applications.

Les sépultures chasséennes du sud de la France

Directory of Open Access Journals (Sweden)

Alain BEYNEIX

2009-12-01

Full Text Available ABSTRACT: A new exam of the «royal» sepulture of Saint-Michel-clu-Touch in Toulouse (dép. Haute- Garonne and a bibliographical study about chassean sepultures in southern France permit to present social aspects of the chassean communities in this area. This study shows on the one hand, a glaring diversity about mortuary practises and rituals, on the other hand, the beginning of a social hiérarchisation. Some sepultures present an architecture or else furnitures which illustre the presence of powerful people in the populations of first farmers in the end of the 5 the millenium and in the beginning of the 4 the millenium B.C. in southern France.RÉSUMÉ: Le réexamen de la sépulture dite «royale» de Saint-Michel-du-Touch à Toulouse (Haute- Garonne suivi d'une étude bibliographique des sépultures chasséennes du sud de la France permettent de relever quelques aspects sociaux des communautés du Néolithique moyen de cette région. Outre de flagrantes diversités de pratiques et de rites funéraires, l'étude de ces sépultures dénote tin début de hiérarchisation sociale. Certaines tombes par leur architecture ou par la qualité de leur mobilier apparaissent comme la preuve tangible d'un phénomène d'émergence du pouvoir au sein des populations de premiers agriculteurs dès la fin du Vo et du début du IV millénaire avant J.C. dans le sud de la France.

Lithuania lifts ban on EU-Russia talks / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2008-01-01

Leedu nõustus loobuma oma vetost Venemaa ja EL-i vahelise strateegilise partnerlusleppe kõneluste alustamisele. Leedu, Poola, Rootsi ja Sloveenia välisministrid otsustasid arvestada kõnelustes ka Leedu tõstatatud probleeme

Adeno-Associated Virus-Mediated Correction of a Canine Model of Glycogen Storage Disease Type Ia

Science.gov (United States)

Weinstein, David A.; Correia, Catherine E.; Conlon, Thomas; Specht, Andrew; Verstegen, John; Onclin-Verstegen, Karine; Campbell-Thompson, Martha; Dhaliwal, Gurmeet; Mirian, Layla; Cossette, Holly; Falk, Darin J.; Germain, Sean; Clement, Nathalie; Porvasnik, Stacy; Fiske, Laurie; Struck, Maggie; Ramirez, Harvey E.; Jordan, Juan; Andrutis, Karl; Chou, Janice Y.; Byrne, Barry J.

2010-01-01

Abstract Glycogen storage disease type Ia (GSDIa; von Gierke disease; MIM 232200) is caused by a deficiency in glucose-6-phosphatase-α. Patients with GSDIa are unable to maintain glucose homeostasis and suffer from severe hypoglycemia, hepatomegaly, hyperlipidemia, hyperuricemia, and lactic acidosis. The canine model of GSDIa is naturally occurring and recapitulates almost all aspects of the human form of disease. We investigated the potential of recombinant adeno-associated virus (rAAV) vector-based therapy to treat the canine model of GSDIa. After delivery of a therapeutic rAAV2/8 vector to a 1-day-old GSDIa dog, improvement was noted as early as 2 weeks posttreatment. Correction was transient, however, and by 2 months posttreatment the rAAV2/8-treated dog could no longer sustain normal blood glucose levels after 1 hr of fasting. The same animal was then dosed with a therapeutic rAAV2/1 vector delivered via the portal vein. Two months after rAAV2/1 dosing, both blood glucose and lactate levels were normal at 4 hr postfasting. With more prolonged fasting, the dog still maintained near-normal glucose concentrations, but lactate levels were elevated by 9 hr, indicating that partial correction was achieved. Dietary glucose supplementation was discontinued starting 1 month after rAAV2/1 delivery and the dog continues to thrive with minimal laboratory abnormalities at 23 months of age (18 months after rAAV2/1 treatment). These results demonstrate that delivery of rAAV vectors can mediate significant correction of the GSDIa phenotype and that gene transfer may be a promising alternative therapy for this disease and other genetic diseases of the liver. PMID:20163245

Utshastnikam i gostjam 5-go Mezhdunarodnogo foruma "Budushtsheje Baltiiskihh portov" = To the participants and guest of the 5th International Forum "The Future of the Baltic ports" / Valerijus Ponomariovas

Index Scriptorium Estoniae

Ponomariovas, Valerijus

2004-01-01

Leedu transpordi- ja kommunikatsiooniministeeriumi aseministri ettekanne 5. rahvusvahelise foorumi "The Future of the Baltic States" avamisel. Baltimaade ning Euroopa Liidu riikide meretranspordialasest koostööst, Leedu transpordipoliitika prioriteetidest, Klaipeda sadama olukorrast, arengust, Leedu sadamaprojektidest

Lithuanian political novice plans massive protests throughout Europe / Juozas Dautartas ; interviewed by Linas Jegelevicius

Index Scriptorium Estoniae

Dautartas, Juozas

2011-01-01

Leedu keskkonnaministeeriumis töötav vastloodud Leedu roheliste partei esimees räägib, et partei plaanib korraldada Valgevenesse Leedu piiri lähedale rajatava tuumaelektrijaama vastaseid meeleavaldusi ja loodab ka parlamenti pääseda

Stable producer cell lines for adeno-associated virus (AAV) assembly.

Science.gov (United States)

Chadeuf, Gilliane; Salvetti, Anna

2010-10-01

Stable producer cell lines containing both the rep and cap genes and recombinant adeno-associated virus (rAAV) vectors can be infected with a helper virus to provide reliable and efficient production of rAAV stocks. However, the development of these cell lines is time-consuming. The procedure described here is therefore recommended only for studies requiring the production of high amounts of rAAV, such as preclinical studies performed in large animals.

Lithuania's first visa from 1990 on exhibit in the Vilnius airport / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

The Economisti ajakirjanik Edward Lucas, kellele väljastati esimene Leedu Vabariigi viisa numbriga 0001, andis selle viisaga passi Leedu välisministeeriumi muuseumile üle. 2011. aasta jooksul eksponeeritakse seda Vilniuse lennujaamas. Edward Lucase arvamusi Leedu poliitikast

Litt ägab tugeva dollari all / Audrius Gelzhinis, Verslo Zhinios

Index Scriptorium Estoniae

Gelzhinis, Audrius

2000-01-01

Leedu liti USA dollarist lahtisidumisega viivitamise tõttu on Leedu jäänud lõksu: tema kaupadel on kõrge hinna tõttu raske konkureerida nii Venemaa kui ka Euroopa Liidu turul. Diagramm: Leedu väliskaubandus 1999

Ex-diplomacy captain slags off the government / Povilas Gylys ; interviewed by Linas Jegelevicius

Index Scriptorium Estoniae

Gylys, Povilas

2011-01-01

Leedu endine välisminister ei taha poliitikasse tagasi tulla, räägib Leedu majandusest ja ei ole rahul sellega, et USA presidendi Barack Obama käest kutse saanud Leedu riigipea Dalia Grybauskaite ei sõitnud Praha kohtumisele

Social issues top election agenda / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Leedu valmistub parlamendivalimisteks 2012. a sügisel. President Grybauskaite hoiatab parlamenti sotsiaalse populismi eest, päevakorral on parteide finantseerimine, loodud kaks uut poliitilist jõudu - Drasos Kelias ja Brasauskiene poolt asutatud Leedu presidendi liit. Leedu parteide võimalustest valimistel

Inflammation and Immune Response of Intra-Articular Serotype 2 Adeno-Associated Virus or Adenovirus Vectors in a Large Animal Model

Directory of Open Access Journals (Sweden)

Akikazu Ishihara

2012-01-01

Full Text Available Intra-articular gene therapy has potential for the treatment of osteoarthritis and rheumatoid arthritis. To quantify in vitro relative gene transduction, equine chondrocytes and synovial cells were treated with adenovirus vectors (Ad, serotype 2 adeno-associated virus vectors (rAAV2, or self-complementary (sc AAV2 vectors carrying green fluorescent protein (GFP. Using 6 horses, bilateral metacarpophalangeal joints were injected with Ad, rAAV2, or scAAV2 vectors carrying GFP genes to assess the in vivo joint inflammation and neutralizing antibody (NAb titer in serum and joint fluid. In vitro, the greater transduction efficiency and sustained gene expression were achieved by scAAV2 compared to rAAV2 in equine chondrocytes and synovial cells. In vivo, AAV2 demonstrated less joint inflammation than Ad, but similar NAb titer. The scAAV2 vectors can induce superior gene transduction than rAAV2 in articular cells, and both rAAV2 and scAAV2 vectors were showed to be safer for intra-articular use than Ad vectors.

Construction of adeno-associated virus packaging plasmids and cells that directly select for AAV helper functions.

Science.gov (United States)

Whiteway, Alistair; Deru, Wale; Prentice, H Grant; Anderson, Robert

2003-12-01

Recombinant adeno-associated virus type 2 (rAAV) has promise for use as a gene therapy vector. Potential problems in the production of rAAV stocks are both the limited amount of recombinant virus that is produced by traditional methods and the possibility of wild-type replication competent adeno-associated virus (wtAAV) contamination. The presence of these contaminants is largely dependent upon the helper plasmid used. Whilst wtAAV is not a pathogen, the presence of these contaminants is undesirable as they may affect experiments concerning the biology of rAAV. Additionally as protocols using rAAV with altered tropism are becoming more prevalent, it is important that no recombination be permitted that may cause the creation of a replication competent AAV with modified (targeting) capsids. Many experimental protocols require the generation of large amounts of high titre rAAV stocks. We describe the production of several AAV helper plasmids and cell lines designed to achieve this goal. These plasmids possess split AAV rep and cap genes to eliminate the production of wtAAV and they possess a selection mechanism which is operatively linked to expression from the AAV cap gene. This allows positive selection of those cells expressing the highest level of the structural capsid proteins and therefore those cells which yield the highest amount of rAAV.

Allocution prononcée par M. François de Rose, Président du Conseil de l'organisation européenne pour la recherche nucléaire à l'occasion de l'inauguration du synchrotron à protons du CERN le 5 février 1960

CERN Multimedia

CERN Press Office. Geneva

1960-01-01

Allocution prononcée par M. François de Rose, Président du Conseil de l'organisation européenne pour la recherche nucléaire à l'occasion de l'inauguration du synchrotron à protons du CERN le 5 février 1960

Adamkus encouraged to grant citizenship to U.S. basketball player

Index Scriptorium Estoniae

2006-01-01

Kuigi uus Leedu kodakondsusseaduse muudatus lubab anda Leedu koondises mängivatele välismaa tippsportlastele Leedu kodakondsuse, lükkas president Valdas Adamkus USA korvpalluri Kathryn Douglas'e kodakondsustaotluse tagasi. Sama ka: Adamkus denies U.S. basketball star citizenship//The Baltic Times 2006, 24-30 aug., lk 4

Viral single-strand DNA induces p53-dependent apoptosis in human embryonic stem cells.

Science.gov (United States)

Hirsch, Matthew L; Fagan, B Matthew; Dumitru, Raluca; Bower, Jacquelyn J; Yadav, Swati; Porteus, Matthew H; Pevny, Larysa H; Samulski, R Jude

2011-01-01

Human embryonic stem cells (hESCs) are primed for rapid apoptosis following mild forms of genotoxic stress. A natural form of such cellular stress occurs in response to recombinant adeno-associated virus (rAAV) single-strand DNA genomes, which exploit the host DNA damage response for replication and genome persistence. Herein, we discovered a unique DNA damage response induced by rAAV transduction specific to pluripotent hESCs. Within hours following rAAV transduction, host DNA damage signaling was elicited as measured by increased gamma-H2AX, ser15-p53 phosphorylation, and subsequent p53-dependent transcriptional activation. Nucleotide incorporation assays demonstrated that rAAV transduced cells accumulated in early S-phase followed by the induction of apoptosis. This lethal signaling sequalae required p53 in a manner independent of transcriptional induction of Puma, Bax and Bcl-2 and was not evident in cells differentiated towards a neural lineage. Consistent with a lethal DNA damage response induced upon rAAV transduction of hESCs, empty AAV protein capsids demonstrated no toxicity. In contrast, DNA microinjections demonstrated that the minimal AAV origin of replication and, in particular, a 40 nucleotide G-rich tetrad repeat sequence, was sufficient for hESC apoptosis. Our data support a model in which rAAV transduction of hESCs induces a p53-dependent lethal response that is elicited by a telomeric sequence within the AAV origin of replication.

Viral single-strand DNA induces p53-dependent apoptosis in human embryonic stem cells.

Directory of Open Access Journals (Sweden)

Matthew L Hirsch

Full Text Available Human embryonic stem cells (hESCs are primed for rapid apoptosis following mild forms of genotoxic stress. A natural form of such cellular stress occurs in response to recombinant adeno-associated virus (rAAV single-strand DNA genomes, which exploit the host DNA damage response for replication and genome persistence. Herein, we discovered a unique DNA damage response induced by rAAV transduction specific to pluripotent hESCs. Within hours following rAAV transduction, host DNA damage signaling was elicited as measured by increased gamma-H2AX, ser15-p53 phosphorylation, and subsequent p53-dependent transcriptional activation. Nucleotide incorporation assays demonstrated that rAAV transduced cells accumulated in early S-phase followed by the induction of apoptosis. This lethal signaling sequalae required p53 in a manner independent of transcriptional induction of Puma, Bax and Bcl-2 and was not evident in cells differentiated towards a neural lineage. Consistent with a lethal DNA damage response induced upon rAAV transduction of hESCs, empty AAV protein capsids demonstrated no toxicity. In contrast, DNA microinjections demonstrated that the minimal AAV origin of replication and, in particular, a 40 nucleotide G-rich tetrad repeat sequence, was sufficient for hESC apoptosis. Our data support a model in which rAAV transduction of hESCs induces a p53-dependent lethal response that is elicited by a telomeric sequence within the AAV origin of replication.

Título do sítio electrónico: Fédération Européenne des Associations Nacionales Travaillant avec les Sans-Abri e European Observatory on Homelessness

Directory of Open Access Journals (Sweden)

João Aldeia

2012-10-01

Full Text Available Optou-se por apresentar conjuntamente as duas páginas pela sua estreita articulação temática e institucional. Criada em 1989, a FEANTSA – Fédération Européenne des Associations Nacionales Travaillant avec les Sans-Abri – é uma organização não-governamental (ONG dedicada a eliminar a pobreza e exclusão social de indivíduos sem-abrigo ou em risco de conhecer esta situação, que congrega mais de 100 ONGs locais e nacionais de vários Estados-membros da União Europeia, sendo financiada pela Comiss...

Neighbors uneasy about radioactive waste proposal / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Lätlased on vastu Leedu plaanile rajada Läti-Leedu piiri lähedusse lühiajaliste radioaktiivsete jäätmete ladustuskoht. Läti keskkonnaminister Raimonds Vejonis saatis oma Leedu kolleegile kirja, milles soovitab leida ettevõtmisele teine koht, kuna lähedal asub Läti jaoks strateegiliselt tähtis turismipiirkond

Toward exascale production of recombinant adeno-associated virus for gene transfer applications.

Science.gov (United States)

Cecchini, S; Negrete, A; Kotin, R M

2008-06-01

To gain acceptance as a medical treatment, adeno-associated virus (AAV) vectors require a scalable and economical production method. Recent developments indicate that recombinant AAV (rAAV) production in insect cells is compatible with current good manufacturing practice production on an industrial scale. This platform can fully support development of rAAV therapeutics from tissue culture to small animal models, to large animal models, to toxicology studies, to Phase I clinical trials and beyond. Efforts to characterize, optimize and develop insect cell-based rAAV production have culminated in successful bioreactor-scale production of rAAV, with total yields potentially capable of approaching the exa-(10(18)) scale. These advances in large-scale AAV production will allow us to address specific catastrophic, intractable human diseases such as Duchenne muscular dystrophy, for which large amounts of recombinant vector are essential for successful outcome.

Convection Enhanced Delivery of Recombinant Adeno-associated Virus into the Mouse Brain.

Science.gov (United States)

Nash, Kevin R; Gordon, Marcia N

2016-01-01

Recombinant adeno-associated virus (rAAV) has become an extremely useful tool for the study of gene over expression or knockdown in the central nervous system of experimental animals. One disadvantage of intracranial injections of rAAV vectors into the brain parenchyma has been restricted distribution to relatively small volumes of the brain. Convection enhanced delivery (CED) is a method for delivery of clinically relevant amounts of therapeutic agents to large areas of the brain in a direct intracranial injection procedure. CED uses bulk flow to increase the hydrostatic pressure and thus improve volume distribution. The CED method has shown robust gene transfer and increased distribution within the CNS and can be successfully used for different serotypes of rAAV for increased transduction of the mouse CNS. This chapter details the surgical injection of rAAV by CED into a mouse brain.

Different effects of the two types of spatial pre-cueing: what precisely is "attention" in Di Lollo's and Enns' substitution masking theory?

Science.gov (United States)

Luiga, I; Bachmann, T

2007-11-01

Enns and Di Lollo [Psychological Science, 8 (2), 135-139, 1997] have introduced the object substitution theory of visual masking. Object substitution masking occurs when focusing attention on the target is delayed. However, Posner (Quarterly Journal of Experimental Psychology, 32, 3-25, 1980) has already shown that attention can be directed to a target at least in two ways: intentionally (endogenously) and automatically (exogenously). We conducted two experiments to explore the effects of endogenous and exogenous cues on substitution masking. The results showed that when attention was shifted to the target location automatically (using a local peripheral pre-cue), masking was attenuated. A decrease in target identification dependent on a delay of mask offset, typical to substitution masking, was not observed. However, strong substitution masking occurred when the target location was not pre-cued or when attention was directed to the target location intentionally (using a symbolic pre-cue displayed centrally). The hypothesis of two different mechanisms of attentional control in substitution masking was confirmed.

Poland's exotics / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Poola viimase aja poliitika on järjest natsionalistlikumaks muutumas ja sündmuste keskel on vähemusrahvused. Viimaste aegade konfliktid Leeduga on seotud uue Leedu haridusseadusega, milles laiendatakse leedu keele kasutust vähemusrahvuste koolides. Poola ekspresident Lech Walesa loobus Leedu ordeni saamisest, põhjendades seda selle seadusega. Kannatada on saanud ka Poolas elavate juutide ja sakslaste kultuuripärand

Pharmacology of Recombinant Adeno-associated Virus Production

Directory of Open Access Journals (Sweden)

Magalie Penaud-Budloo

2018-03-01

Full Text Available Recombinant adeno-associated viral (rAAV vectors have been used in more than 150 clinical trials with a good safety profile and significant clinical benefit in many genetic diseases. In addition, due to their ability to infect non-dividing and dividing cells and to serve as efficient substrate for homologous recombination, rAAVs are being used as a tool for gene-editing approaches. However, manufacturing of these vectors at high quantities and fulfilling current good manufacturing practices (GMP is still a challenge, and several technological platforms are competing for this niche. Herein, we will describe the most commonly used upstream methods to produce rAAVs, paying particular attention to the starting materials (input used in each platform and which related impurities can be expected in final products (output. The most commonly found impurities in rAAV stocks include defective particles (i.e., AAV capsids that do contain the therapeutic gene or are not infectious, residual proteins from host cells and helper viruses (adenovirus, herpes simplex virus, or baculoviruses, and illegitimate DNA from plasmids, cells, or helper viruses that may be encapsidated into rAAV particles. Given the role that impurities may play in immunotoxicity, this article reviews the impurities inherently associated with each manufacturing platform.

Unrest spoils Polish Independence Day / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Poola ultraparempoolsete meeleavaldusest Poola iseseisvuspäeva pidustustel, mis oli suunatud Leedu, juutide, homoseksualismi ja feminismi vastu; poolakeelsest haridusest Leedus, Leedu kogukonnast Poolas

With the black market booming, alcohol producers focus on exports / Linas Jegelevicius

Index Scriptorium Estoniae

Jegelevicius, Linas

2010-01-01

Alkoholi salakaubanduse põhjuseks on Leedu suur aktsiisimaks. President Dalia Grybauskaite pani veto aktsiisiseaduse parandustele. Leedu suurimatest alkoholitootjatest Vilniaus Degtine ja Mineraliniai Vandenys

Right wing parties join forces / Louise O'Dwyer

Index Scriptorium Estoniae

O'Dwyer, Louise

2008-01-01

Leedu konservatiivne Isamaaliit ja Kristlik-Demokraatlik Partei kirjutasid ühiskongressil alla kahe partei ühinemiskokkuleppele. Uue poliitilise jõu nimetuseks on Isamaaliit - Leedu Kristlikud Demokraadid

Sotsiaalmeedia osatähtsus PR tööriistana kasvab : Baltimaade, Poola ja Valgevene suhtekorraldajad / Villy Paimets

Index Scriptorium Estoniae

Paimets, Villy, 1972-

2016-01-01

Leedu suhtekorraldajate ühendus tegi uuringu Leedu ja tema naaberriikide PR turgude hetkeseisust ja tulevikuväljavaadetest. Põhjalikumalt uuriti Eesti, Läti, Leedu, Poola ja Valgevene turge. Uuringu tulemuste kohta avaldavad arvamust SEB kommunikatsioonijuht Evelin Allas, PR-ettevõtja ja -õppejõud Mart Soonik ning kaitseväe meediasuhete juhina ja suhtekorraldusfirmas PR Partner strateegilise konsultandina töötanud Riigikogi IRL-i fraktsiooni meedianõunik Ingrid Mühling

Kirkilas appeals to patriotism of private energy firm

Index Scriptorium Estoniae

2007-01-01

Leedu peaminister kutsub kolme Leedu energeetikaettevõtet ühinema ehitamaks uut tuumaelektrijaama. Valitsus pole rahul ühinevate ettevõtete varade hinnanguga, mille tegi audiitorfirma KPMG Baltics

Kaitseliit ja sõsarorganisatsioonid / Romualdas Moldaris, Harri Kainulainen

Index Scriptorium Estoniae

Moldaris, Romualdas

2010-01-01

Kaitseliidu 92. aastapäeval külastasid Kaitseliitu Leedu, Soome ja Taani sõsarorganisatsioonid. Intervjuud Leedu vabatahtliku riigikaitseorganisatsiooni KASP ülema ja Soome Riigikaitsekoolitusliidu esimehega

The European City in the Age of Globalisation La ville européenne à l'ère de la globalisation

Directory of Open Access Journals (Sweden)

Robert Musil

2012-12-01

Full Text Available This conceptual paper identifies a gap between two meaningful fields of research in urban geography : the first, old-established and reinforced by the European integration process, asks for recent and future trends, primarily on intra-urban development of cities on the European scale. The second focuses on inter-urban relations on a global scale, inspired by global city theories established since early 1990s. As a consequence of this research gap, a research agenda has been formulated with the aim to deliver the missing links between these two fields. Therein, three methodological improvements are put forward : first, more research has to be done to prove the widely accepted thesis of an existing link between global integration and internal urban structure. Second, the role of specific national politics for the production of a global city has to be examined – such as the type of welfare-regime or the impact of historical political structures. Finally, global city research should seriously consider the territorial arrangements that impinge upon the development of global cities on a national and a European scale.Cet article conceptuel identifie un écart entre deux grands domaines de recherche en géographie urbaine : le premier, établi de longue date et renforcé par le processus d'intégration européen, se concentre principalement sur les tendances récentes ou à venir en matière de développement intra-urbain à l'échelle européenne. Le second pose la question des relations inter-urbaines à l'échelle globale, née des théories sur la Ville Globale échafaudées depuis le début des années 1990. Au vu de cette lacune, un programme de recherche a été mis en place afin de fournir les chaînons manquants entre ces deux domaines. A cet égard, trois améliorations méthodologiques sont proposées : la première est la nécessité d'accroître le volume des recherches pour prouver la thèse largement admise d'un lien entre int

Single residue AAV capsid mutation improves transduction of photoreceptors in the Abca4-/- mouse and bipolar cells in the rd1 mouse and human retina ex vivo.

Science.gov (United States)

De Silva, Samantha R; Charbel Issa, Peter; Singh, Mandeep S; Lipinski, Daniel M; Barnea-Cramer, Alona O; Walker, Nathan J; Barnard, Alun R; Hankins, Mark W; MacLaren, Robert E

2016-11-01

Gene therapy using adeno-associated viral (AAV) vectors for the treatment of retinal degenerations has shown safety and efficacy in clinical trials. However, very high levels of vector expression may be necessary for the treatment of conditions such as Stargardt disease where a dual vector approach is potentially needed, or in optogenetic strategies for end-stage degeneration in order to achieve maximal light sensitivity. In this study, we assessed two vectors with single capsid mutations, rAAV2/2(Y444F) and rAAV2/8(Y733F) in their ability to transduce retina in the Abca4 -/- and rd1 mouse models of retinal degeneration. We noted significantly increased photoreceptor transduction using rAAV2/8(Y733F) in the Abca4 -/- mouse, in contrast to previous work where vectors tested in this model have shown low levels of photoreceptor transduction. Bipolar cell transduction was achieved following subretinal delivery of both vectors in the rd1 mouse, and via intravitreal delivery of rAAV2/2(Y444F). The successful use of rAAV2/8(Y733F) to target bipolar cells was further validated on human tissue using an ex vivo culture system of retinal explants. Capsid mutant AAV vectors transduce human retinal cells and may be particularly suited to treat retinal degenerations in which high levels of transgene expression are required.

V parke Tuvi mozhet pojavitsja angel / Urmas Seaver

Index Scriptorium Estoniae

Seaver, Urmas, 1973-

2008-01-01

Leedu skulptori Vaidas Ramoshka kiviskulptuur "Ingel" pannakse Eesti Rahvusraamatukogu taga asuvasse Tuvi parki. Leedu pealinna Vilniuse üks sümbol-kujudest kingitakse Tallinnale kui tulevasele kultuuripealinnale

EU commissioner advises introducing euro "when ready" / Arturas Racas

Index Scriptorium Estoniae

Racas, Arturas

2006-01-01

Euroopa Liidu rahandusasjade volinik Joaquin Almunia teatas, et Leedu ei peaks lootma kriteeriumite muutmisele eurole üleminekul. Leedu rahandusminister pakub eurotsooniga liitumise tähtajaks aastat 2010

Brussels questions power grid sell-off

Index Scriptorium Estoniae

2007-01-01

Euroopa Komisjon palus Leedu majandusministeeriumilt selgitust Lääne-Leedu elektrivõrgufirma Vakaru Skirstomieji Tinklai erastamise kohta. Peaminister Kirkilas kaitseb otsust ühendada kolm energiakompaniid üheks

MEP : Political parties funded by bribes / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2004-01-01

Leedu Sotsiaaldemokraatliku Partei liikme, Euroopa Parlamendi saadiku Aloyzas Sakalase hinnangul finantseeritakse Leedu parteisid altkäemaksudest ja korruptsioonis süüdistatavad parlamendiliikmed tegutsesid oma parteide nimel

Uspaski̜h repeats mistakes of Paksas / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu parlamendi opositsioon on vastu majandusminister Viktor Uspaskihh'i tegevusele isiklikus äris. Ametlike dokumentide kohaselt tegi minister Moskva visiidi ajal ettepaneku luua Leedu-Venemaa ühisettevõte

Teatras 47 blends paganism with folk art / Kimberly Kweder

Index Scriptorium Estoniae

Kweder, Kimberly

2007-01-01

Vilniuses, Leedu Teatri-, Muusika- ja Filmimuuseumis avati tuntud leedu teatrikunstniku Dalia Mataitiene näitus Teatras 47. Kommenteerivad muuseumi direktor Regina Lopiene ja muuseumi Teatriosakonna juht Aukse Kapociute

Foreign Minister angers conservatives over compensation / Laima Vaiga

Index Scriptorium Estoniae

Vaiga, Laima

2008-01-01

Energiaressursside tagamise huvides ei soovita Leedu välisminister Petras Vaitiekunas valitsusel aktiivselt nõuda Venemaalt okupatsioonikahjude hüvitamist. Kahjude hüvitamist nõuavad Leedu konservatiivid

Lithuania's utility chief lashes out at Poland, Latvia

Index Scriptorium Estoniae

2008-01-01

Lietuvos Energija tegevjuht Rymantas Juozaitis kritiseeris Poolat ja Lätit viivitamise pärast energiasilla rajamisel Leedu ja Poola ning Läti ja Rootsi vahel. Aleksandras Abisala Leedu energiavarudest

The planes are back / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2009-01-01

2009. aasta alguses pankrotistus Leedu lennufirma FlyLAL, kuid novembris selgus, et Air Baltic ja Leedu lennukompanii Star1 Airlines alustavad uute regulaarlendudega Vilniusest. Leedus on 3 rahvusvahelist lennujaama

Induction of immunity to antigens expressed by recombinant adeno-associated virus depends on the route of administration.

Science.gov (United States)

Brockstedt, D G; Podsakoff, G M; Fong, L; Kurtzman, G; Mueller-Ruchholtz, W; Engleman, E G

1999-07-01

Recombinant adeno-associated virus (rAAV) is a replication-defective parvovirus which is being explored as a vector for gene therapy because of its broad host range, excellent safety profile, and durable transgene expression in infected hosts. rAAV has also been reported by several groups to induce little or no immune response to its encoded transgene products. In this study we examined the immunogenicity of rAAV by studying the immune response of C57BL/6 mice to a single dose of rAAV-encoding ovalbumin (AAV-Ova) administered by a variety of routes. Mice injected with AAV-Ova intraperitoneally (ip), intravenously, or subcutaneously developed potent ovalbumin-specific cytotoxic T lymphocytes (CTL) as well as anti-ovalbumin antibodies and antibodies to AAV. In contrast, mice injected with AAV-Ova intramuscularly developed a humoral response to the virus and the transgene but minimal ovalbumin-specific CTLs. The induced CTL response after ip administration of AAV-Ova protected mice against a subsequent tumor challenge with an ovalbumin-transfected B16 melanoma cell line. Studies of the mechanism by which AAV-Ova induces CTL confirmed that the virus delivers the transgene product into the classical MHC class I pathway of antigen processing. Mice that previously had been exposed to rAAV vectors failed to develop ovalbumin-specific CTL following administration of AAV-Ova. Analysis of these mice revealed the presence of circulating anti-AAV antibodies that blocked rAAV transduction in vitro and inhibited CTL induction in vivo. These results suggest a possible role for rAAV in the immunotherapy of malignancies and viral infections, although induced antibody responses to AAV may limit its ability to be administered for repeated vaccinations. Copyright 1999 Academic Press.

Lithuania eyeing Swedish, Central European grid

Index Scriptorium Estoniae

2006-01-01

Leedu valitsus kordas, et ühendab Leedu elektrivõrgu Rootsi, Poola ja Tsehhi süsteemidega. Ka Soome elektrivõrk ühendatakse Balti riikidega. Tabel: Millistest allikatest tuleb Baltimaade elektrienergia

Komorowski celebrates Lithuanian holiday in Vilnius / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Poola president Bronislaw Komorowski võttis osa 16. veebruaril peetud Leedu riigi taastamise päeva pidustustest. Poola-Leedu vahelistest probleemidest ja Poola välisministri Radoslaw Sikorski osast selles

TEO - vana rahamasin töötab ikka veel / Steffan Andersson

Index Scriptorium Estoniae

Andersson, Steffan

2006-01-01

Ülevaade Leedu kommunikatsioonifirma TEO (endine Leedu Telekom, praegu 60% mahus TeliaSonera tütarfirma) majandustegevusest ja aktsia hinnast. Graafik: TEO aktsia kurss 24. okt. 2005 - 24. okt. 2006

Mycotoxin production by Fusarium avenaceum strains isolated from Norwegian grain and the cytotoxicity of rice culture extracts to porcine kidney epithelial cells.

Science.gov (United States)

Morrison, Ellen; Kosiak, Barbara; Ritieni, Alberto; Aastveit, Are H; Uhlig, Silvio; Bernhoft, Aksel

2002-05-08

The secondary metabolites of 24 isolates of Fusarium avenaceum from Norwegian cereals and grown on rice have been characterized. Moniliformin (MON), enniatins (ENNs), and beauvericin (BEA) were analyzed by high-performance liquid chromatography. Porcine kidney epithelial cells (PK15, American Type Culture Collection) were used to study the cytotoxicity of MON in the extracts. The following metabolites were produced by all isolates, ranked by concentration in rice cultures: ENN-B, MON, ENN-B1, and ENN-A. BEA was produced by eight isolates. The productions of BEA and ENN-A were significantly correlated, as was the case with ENN-B and ENN-B1. MON production was correlated neither to any of the other toxins nor to toxicity.

Echo of the Latvian political turmoil in Lithuania / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Leedu suursaadik Lätis Antanas Valionis kritiseeris Läti presidenti Valdis Zatlersit pärast tema 28. mai kõne ja leidis, et uued parlamendivalimised Lätis võivad venelastele suunatud jõududele kasulikud olla, samuti on praegune segadus Läti poliitikas halb riigi majanduse ja investeerimiskliima jaoks. 31. mail kohtus Antanas Valionis Leedu presidendi Dalia Grybauskaitega ja seejärel vabandas Valdis Zatlersi ees. Lätis elava Leedu filosoofi Arvydas Juozaitise raamatust "Riga - the civilization of nobody"

Maldeikis resigns amid storm of protest / Rokas M Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M

2001-01-01

Leedu majandusminister esitas tagasiastumispalve pärast skandaali, kui sai teatavaks, et tema Moskva-visiidi organiseerimises ja kohtumisel Venemaa nafta- ja gaasikompaniide juhtidega osalesid erafirmad, kes on huvitatud Leedu gaasikompanii erastamisest

Lithuania manages to steer clear of IMF loans

Index Scriptorium Estoniae

2009-01-01

Leedu peaministri Andrius Kubiliuse sõnul saab Leedu finantssüsteem rahvusvahelistel finantsturgudel kindlust juurde, mis tähendab, et riik võib hakkama saada ilma IMF-i abita. Peaminister eelarvepoliitikast

Methods of treating Parkinson's disease using viral vectors

Energy Technology Data Exchange (ETDEWEB)

Bankiewicz, Krystof; Cunningham, Janet

2016-11-15

Methods of delivering viral vectors, particularly recombinant adeno-associated virus (rAAV) virions, to the central nervous system (CNS) using convection enhanced delivery (CED) are provided. The rAAV virions include a nucleic acid sequence encoding a therapeutic polypeptide. The methods can be used for treating CNS disorders such as for treating Parkinson's Disease.

Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences

Directory of Open Access Journals (Sweden)

Dilley Robert

2009-01-01

Full Text Available Abstract Background Adeno-associated virus (AAV vectors are promising tools for gene therapy. Currently, their potential is limited by difficulties in producing high vector yields with which to generate transgene protein product. AAV vector production depends in part upon the replication (Rep proteins required for viral replication. We tested the hypothesis that mutations in the start codon and upstream regulatory elements of Rep78/68 in AAV helper plasmids can regulate recombinant AAV (rAAV vector production. We further tested whether the resulting rAAV vector preparation augments the production of the potentially therapeutic transgene, insulin-like growth factor I (IGF-I. Results We constructed a series of AAV helper plasmids containing different Rep78/68 start codon in combination with different gene regulatory sequences. rAAV vectors carrying the human IGF-I gene were prepared with these vectors and the vector preparations used to transduce HT1080 target cells. We found that the substitution of ATG by ACG in the Rep78/68 start codon in an AAV helper plasmid (pAAV-RC eliminated Rep78/68 translation, rAAV and IGF-I production. Replacement of the heterologous sequence upstream of Rep78/68 in pAAV-RC with the AAV2 endogenous p5 promoter restored translational activity to the ACG mutant, and restored rAAV and IGF-I production. Insertion of the AAV2 p19 promoter sequence into pAAV-RC in front of the heterologous sequence also enabled ACG to function as a start codon for Rep78/68 translation. The data further indicate that the function of the AAV helper construct (pAAV-RC, that is in current widespread use for rAAV production, may be improved by replacement of its AAV2 unrelated heterologous sequence with the native AAV2 p5 promoter. Conclusion Taken together, the data demonstrate an interplay between the start codon and upstream regulatory sequences in the regulation of Rep78/68 and indicate that selective mutations in Rep78/68 regulatory elements

Repse frightens at flight controls

Index Scriptorium Estoniae

2003-01-01

Leedu meedia teatel püüdis Läti peaminister Einars Repshe sõjaväekopteri juhtimisega Leedu presidendile Rolandas Paksasele muljet avaldada. Paksas osales Lätis Euroopa Liitu astumist toetavas kampaanias

Discussing nuclear energy / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Venemaa ja Valgevene allkirjastasid peaminister Vladimir Putini visiidi ajal kokkuleppe uue tuumajaama ehitamiseks Valgevene ja Leedu piiri lähistele. Mõeldes Jaapanis hiljuti juhtunud tuumaõnnetusele ei ole Leedu selle otsusega rahul

Findings in the alleged CIA prison / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Leedu parlamendi julgeolekukomitee kinnitas, et Leedus eksisteerisid CIA salavanglad, kuid ei ole kindlaks tehtud, kas seal on terrorismis kahtlustatavaid kinni hoitud. Leedu suursaadik Gruusias Mecys Laurinkus on ametist vabastatud seoses usalduse kaotusega

Power link fiasco comes to a head / Kate McIntosh

Index Scriptorium Estoniae

McIntosh, Kate

2009-01-01

Elektrikaablit Rootsiga soovivad rajada nii Läti kui ka Leedu. Läti, Leedu ja Rootsi välisministrid on arvamusel, et elektrikaabli rajamine Skandinaavia ja Baltikumi vahel on oluline ja peaks olema prioriteetne

Center-right coalition to lead Lithuania / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2008-01-01

Neli Leedu parteid sõlmisid koalitsioonileppe. Valitsust hakkab moodustama Isamaliidu ja Leedu Kristlike Demokraatide juht Andrius Kubilius. Uus koalitsioon kavatseb muuta riigieelarve seaduseelnõud ning vältida 2009. aasta riigieelarve defitsiidi tekkimist

Baltic PMs urge support for EU

Index Scriptorium Estoniae

2003-01-01

Eesti peaministri Juhan Partsi, Läti peaministri Einars Repshe ja Leedu peaministri Algirdas Brazauskase ühisavaldusest Eestile ning Lätile järgimaks Leedu eeskuju referendumil EL-i poolt hääletamises

Limits of free speech to be tested / Matt Kovalick

Index Scriptorium Estoniae

Kovalick, Matt

2003-01-01

Euroopa Inimõiguste Kohtu arupärimisest Leedu justiitsministeeriumile seoses sõnavabaduse väidetava rikkumisega Danute Balsyte-Lideikiene poolt välja antud 2000. aasta Leedu kalendri konfiskeerimisel, mis levitas natsionalistlikke ideid

Lithuania's new Cold War / Egdunas Racius

Index Scriptorium Estoniae

Racius, Egdunas

2004-01-01

Autori hinnangul varjutavad Leedu poliitikat jätkuvalt külma sõja aegsed arusaamad ja Leedu poliitilises spektris suudetakse eristada vaid kahte rühma - läänemeelseid ja venemeelseid poliitikuid

Kirkilas demands bureaucrats get to bottom of capricious milk market

Index Scriptorium Estoniae

2008-01-01

Leedu peaminister Gediminas Kirkilas nõuab Leedu põllumajandusministeeriumilt ja konkurentsiametilt meetmeid riigi piimaturu stabiliseerimiseks. Piima kokkuostuhind on langenud, samas kui jaemüügihinnad jäid samaks või tõusid

Diplomats defend Snaige in duty row

Index Scriptorium Estoniae

2005-01-01

Leedu soovib, et Ukraina tühistaks uued tollimaksud Euroopa Liidus valmistatud kodukaupadele. Leedu kodumasinate tootja Snaige kavatseb hakata eksportima oma tooteid just Ukrainasse ja suurendada müüki Venemaale ja Valgevenesse

Polish PM visits Lithuanian amidst anti-Lithuanian hysteria in Poland / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Poola peaminister Donald Tusk saabus Palangasse, et kohtuda Leedu peaministri Andrius Kubiliusega ja rääkida temaga Leedu hariduspoliitikast. Samuti pidas ta Vilniuses asuvas Püha Helena kirikus Leedus elavatele poolakatele kõne

Jewish community indignant over press, legislation

Index Scriptorium Estoniae

2005-01-01

Ameerika Juudikomitee rahvusvaheliste küsimuste direktor Andrew Baker avaldas pahameelt, et prokuratuur lõpetas uurimise Leedu päevalehe antisemitistlike artiklite suhtes. Leedu juudi kogukond esitas valitsusele nimekirja ehitistest, mille tagastamist nad soovivad

"Videvik" - hiiglaslik videotuba / Hanno Soans

Index Scriptorium Estoniae

Soans, Hanno, 1974-

1998-01-01

Sorosi Kaasaegse Kunsti Leedu Keskuse V aastanäitus "Videvik" Vilniuse keskuses 20. jaan. - 22. veebr. Korraldajad leedu noorema põlvkonna kriitikud-kuraatorid K. Kuizinas, D. Narkevicius, E. Stankevicius, briti osa kordinaator R. Wall.

Mazeikiai refinery woes deepen on all fronts

Index Scriptorium Estoniae

2007-01-01

Leedu rahandusminister teatas, et riigieelarvet mõjutavad probleemid Mazeikiai tehasega, mis on Leedu suurim maksumaksja. Lisa: Russian oil company to build refinery. Surgutneftegaz teatas plaanist ehitada naftatöötlustehas Peterburi lähedale

Report: Paksas scandal raises privacy concerns / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2004-01-01

Inimõiguste ekspertide uuring näitab, et nii Leedu presidendi Rolandas Paksase administratsiooni töötajad, presidendi tegevuse uurijad kui ka Leedu ajakirjandus rikkusid mitmeid inimõigusi, eelkõige aga õigust privaatsusele

Enniatin A1, enniatin B1 and beauvericin on HepG2: Evaluation of toxic effects.

Science.gov (United States)

Juan-García, Ana; Ruiz, María-José; Font, Guillermina; Manyes, Lara

2015-10-01

Hepatotoxicity of three Fusarium mycotoxins, beauvericin (BEA) and two enniatins (ENNs) ENN A1 and ENN B1, in hepatocarcinoma cells (HepG2) were evaluated and compared. Concentrations used were 1.5 and 3 μM at 24, 48 and 72 h for each mycotoxin. Flow cytometry was used to examine enniatins effects on cell proliferation, to characterize the cell cycle phase where the cells blocked and to study the mitochondria role in ENNs-induced apoptosis. ENN B1 treated cells showed a time dependent G1 blockade at both concentrations used. ENN A1 and BEA decreased the apoptotic-necrotic percentage of cells comparing to control and disrupted the MMP as observed by TMRM and ToPro-3 fluorochromes signal. It is proposed a decreasing mycotoxin order by number of effects as follows: BEA > ENN B1 > ENN A1, with 47, 20 and 16%, respectively out of all situations compared. Copyright © 2015 Elsevier Ltd. All rights reserved.

Looking for alternative energy supplies / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Vilniuses toimunud Balti Arengufoorumil räägiti energeetika teemadel. Euroopa Komisjoni presidendi Jose Manuel Barroso ja Leedu presidendi Dalia Grybauskaite kohtumisel räägiti Leedu-Poola gaasijuhtme rajamisest ja Euroopa integreeritud elektrienergiaturu loomisest

Echo of the Muslim world's events / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Osama bin Ladeni surm ei pakkunud Leedu meediale suurt huvi. Leedu välisminister Audronius Azubalis leiab, et Osama bin Ladeni tabamine toimus valel ajal, sest just praegu on hakanud islamiusulised vabaduse eest võitlema

Talks on nuclear plant break down

Index Scriptorium Estoniae

2007-01-01

Kasvavad probleemid Leedu tuumaelektrijaama projektiga. Läbirääkimistest Leedu valitsuse ja NDX Energija vahel. DnB Nord Bankas nimetas projekti liiga riskantseks ja kulukaks. Lisa: Eesti Energia board member suggests plant for Estonia

Mirage vs. Albatross - collision in the air / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

30. augustil põrkasid Leedus kokku NATO õhuturbemissioonil olev Prantsuse hävituslennuk Mirage 2000c ja Leedu õppehävitaja L-39ZA Albatross. Leedu piloodid katapulteerusid, Prantsusmaa piloot katapulteerumist ei vajanud. Ohvreid ei olnud

Putin and Kubilius in energy talks

Index Scriptorium Estoniae

2010-01-01

Leedu peaministri Andrius Kubiliuse ja Venemaa peaministri Vladimir Putini kohtumisel räägiti energeetikast. Andrius Kubilius rõhutas, et Leedu kavatseb ehitada tuumaelektrijaama Visaginasesse ja ei plaani teistest regionaalsetest energeetika-alastest projektidest osa võtta

Reports : PKN Orlen may revive power link, Geonafta

Index Scriptorium Estoniae

2006-01-01

Poola firma PKN Orlen, mis ostab Mazeikiu Nafta, alustab ka Leedu ja Poola elektrienergiat ühendava projektiga. Poola ettevõtte on huvitatud investeerimisest Leedu naftaettevõttesse Geonafta. Vt. samas: Kremlit pahandab Mazeikiu Nafta müük

Avati sõjalooline näitus "War after War" / Ülo Ignats ; foto: Ülo Ignats

Index Scriptorium Estoniae

Ignats, Ülo, 1951-2011

2007-01-01

Leedu president Valdas Adamkus avas näituse Leedu teisest vabadussõjast pealkirjaga "War after War" (Sõda pärast sõda) tseremooniaga Stockholmi linnamajas (Stadshuset). President Adamkuse kõnest. Rootsi haridusminister Jan Björklundi ettekandest

Chronic Dietary Intake of Enniatin B in Broiler Chickens Has Low Impact on Intestinal Morphometry and Hepatic Histology, and Shows Limited Transfer to Liver Tissue

Directory of Open Access Journals (Sweden)

Sophie Fraeyman

2018-01-01

Full Text Available The Fusarium mycotoxin enniatin B (ENN B is a so-called emerging mycotoxin frequently contaminating poultry feed. To investigate the impact of chronic ENN B exposure on animal health, broiler chickens were fed either a diet naturally contaminated with ENN B (2352 µg/kg or a control diet (135 µg/kg for 2, 7, 14, or 21 days. ENN B concentrations were determined in plasma and liver using a validated ultra-high performance liquid chromatography—tandem mass spectrometry UHPLC-MS/MS method. Liver was evaluated histologically, and the villus length and crypt depth of the duodenum, jejunum, and ileum were measured. Histopathology of the livers did not reveal major abnormalities. Feeding an ENN B-contaminated diet could possibly inhibit the proliferation of enterocytes in the duodenal crypts, but did not affect villus length, crypt depth, or villus length-crypt depth ratio of the jejunum and ileum. ENN B levels in plasma and liver were significantly higher in the ENN B-fed group and ranged between <25–264 pg/mL and <0.05–0.85 ng/g, respectively. ENN B carry-over rates from feed to liver tissue were 0.005–0.014% and 0.034–0.109% in the ENN B and control group, respectively. Carry-over rates were low and indicated a limited contribution of poultry tissue-derived products to the total dietary ENN B intake for humans. The above results support the opinion of the European Food Safety Authority stating that adverse health effects from ENN B in broiler chickens are unlikely.

The bully on the Vistula / Kestutis K. Girnius

Index Scriptorium Estoniae

Girnius, Kestutis K.

2011-01-01

Leedut külastanud Poola peaminister Donald Tusk ütles, et Leedu ja Poola vahelised suhted hakkavad sõltuma Leedu suhtumisest seal elavatesse poolakatesse, kuid pressikonverentsil kinnitasid mõlema riigi peaministrid, et küsimust ei tohi politiseerida

Lithuania will build its liquid gas terminal / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

21. juulil otsustas Leedu valitsus, et Klaipedasse ehitatakse veeldatud maagaasi terminal. See otsus lõpetab Gazpromi monopoli. 28. juunil toimunud Valgevene presidendi Aleksander Lukashenko ja Leedu peaministri Andrius Kubiliuse kohtumisel räägiti samuti veeldatud maagaasi terminalidest

Zatlers discusses energy issues in Lithuania / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

10. veebruaril alanud Läti presidendi Valdis Zatlersi Leedu riigivisiidist, mille käigus ta kohtus ka Leedu presidendi Dalia Grybauskaitega. Kohtumisel räägiti energeetika teemadel ja rõhutati, et riikidevahelised suhted on jätkuvalt head

Hygiène des denrées alimentaires d'origine animale - Synthèse I : Développement actuel du concept de l'hygiène en agro-alimentaire et des exigences des normes européennes.

OpenAIRE

Vindevogel, Henri; Daube, Georges; Korsak Koulagenko, Nicolas; Hans, J. C.; Denys, J.; Ghafir, Y.

1996-01-01

Cette synthèse fait partie d’une série de trois articles qui présentent une mise au point des concepts et normes actuels au niveau européen en industrie agro-alimentaire : - Synthèse I : « Développement actuel du concept de l’hygiène en agro-alimentaire et des exigences des normes européennes » ; - Synthèse II : « Vers une assurance qualité intégrée de la chaîne agro-alimentaire » ; - Synthèse III : « Le vétérinaire et la qualité microbiologique des denrées alimentaires d’origine ani...

TBT recommends : Nomme jazz spring concert. Easter celebration. Hakan Rosengren in concert

Index Scriptorium Estoniae

2006-01-01

Inglise jazzlaulja Claire Fosteri ja trompetisti Paul Jayasinha kontserdist 8. apr. Nõmme kultuurimajas Tallinnas. Kontsertidest 16. ja 17. apr. Läti Etnograafiamuuseumis ning Usma kirikus. Rootsi klarnetisti Hakan Rosengreni ja Leedu Rahvusliku Sümfooniaorkestri kontserdist Leedu Rahvusliku Filharmoonia Hallis Vilniuses

Maaelu elavdamise miljonid seisid rämpsvõlakirjades / Mirko Ojakivi

Index Scriptorium Estoniae

Ojakivi, Mirko

2010-01-01

Maaelu arendamise sihtasutus soetas ligi 33 mln. krooni väärtuses Leedu riigi võlakirju 2009. a. kevadel, mil uudisteagentuuri Bloomberg teatel olid nii Läti kui ka Leedu võlakirjad maailma riskantseimate riigivõlakirjade esikümnes. Diagrammid, tabelid

German troops to stay in Lithuania until security situation changes

Index Scriptorium Estoniae

2017-01-01

NATO pataljoni kuuluvad Saksa sõdurid jäävad Leetu kuni julgeolekuolukorra paranemiseni. Saksamaa ja Leedu sõjaline koostöö on viimastel aastatel kasvanud: Leedu on Saksamaalt ostnud märkimisväärsel hulgal relvastust ja sõjatehnikat

Head-count cut is necessary to boost responsibility of public authorities / Alvydas Medalinskas ; interviewed by Linas Jegelevicius

Index Scriptorium Estoniae

Medalinskas, Alvydas

2011-01-01

Leedu kunagise poliitilise liikumise Sajudis liige räägib liikumisest, oma poliitilisest tegevusest, leiab, et praeguse Leedu suurimad saavutused on liitumine EL-i ja NATO-ga ning ta ei usu, et Andrius Kubilius jätkab peaministrina ka pärast parlamendivalimisi

Tallinna Kunstihoones on skriinseiver

Index Scriptorium Estoniae

2003-01-01

21. II-16. III eesti-läti-leedu-inglise ühisprojekt "Skriinseiver". Projekti eesotsas on kuraatorid Anders Härm, Hanno Soans ja troubleproduction. Esinevad Eriks Bozhis (Läti), Darius Mikshys (Leedu), John Frankland (Inglismaa), troubleproductions, ESTO TV ja 11 osalejat Eestist

One more piece of KGB puzzle put in place

Index Scriptorium Estoniae

2005-01-01

Leedu parlamendi erikomisjon leidis, et KGB reservi arvatud isikute staatus erineb teistest organisatsiooni töötajate ja salaagentide omast. Komisjon jõudis järeldusele, et Antanas Valionise ja Arvydas Pociuse kunagine kuulumine KGB reservi ei kujuta endast ohtu Leedu rahvuslikule julgeolekule.

Fluxuse "tagasitulek" Leetu = The "return" of Fluxus to Lithuania / Petra Stegmann ; interv. Skaidra Trilupaityte

Index Scriptorium Estoniae

Stegmann, Petra

2008-01-01

Näitus "Fluxus East. Fluxuse võrgustikud Ida-Euroopas" Kumu Kunstimuuseumis, kuraator Petra Stegmann. Fluxuse liikumisest leedu kunstniku Yurgis George Maciunase eestvedamisel. Rühmituse ideoloogiast, liikmete tegevusest. Vilniuses asutatud Jonas Mekase Visuaalkunsti Keskuse poolt omandatud leedu kunstnike töödest, nende uurimisest

Baltic leaders celebrate the Baltic Way anniversary / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2009-01-01

Eesti peaminister Andrus Ansip, Läti peaminister Valdis Dombrovskis ja Leedu peaminister Andrius Kubilius tähistasid 23. augustil Vilniuses Balti keti kahekümnendat aastapäeva. Üritustest võtsid osa ka Leedu president Dalia Grybauskaite ja Baltimaade parlamentide esimehed

Improved Extension Neural Network and Its Applications

Directory of Open Access Journals (Sweden)

Yu Zhou

2014-01-01

Full Text Available Extension neural network (ENN is a new neural network that is a combination of extension theory and artificial neural network (ANN. The learning algorithm of ENN is based on supervised learning algorithm. One of important issues in the field of classification and recognition of ENN is how to achieve the best possible classifier with a small number of labeled training data. Training data selection is an effective approach to solve this issue. In this work, in order to improve the supervised learning performance and expand the engineering application range of ENN, we use a novel data selection method based on shadowed sets to refine the training data set of ENN. Firstly, we use clustering algorithm to label the data and induce shadowed sets. Then, in the framework of shadowed sets, the samples located around each cluster centers (core data and the borders between clusters (boundary data are selected as training data. Lastly, we use selected data to train ENN. Compared with traditional ENN, the proposed improved ENN (IENN has a better performance. Moreover, IENN is independent of the supervised learning algorithms and initial labeled data. Experimental results verify the effectiveness and applicability of our proposed work.

Involvement of enniatins-induced cytotoxicity in human HepG2 cells.

Science.gov (United States)

Juan-García, Ana; Manyes, Lara; Ruiz, María-José; Font, Guillermina

2013-04-12

Enniatins (ENNs) are mycotoxins found in Fusarium fungi and they appear in nature as mixtures of cyclic depsipeptides. The ability to form ionophores in the cell membrane is related to their cytotoxicity. Changes in ion distribution between inner and outer phases of the mitochondria affect to their metabolism, proton gradient, and chemiosmotic coupling, so a mitochondrial toxicity analysis of enniatins is highly recommended because they host the homeostasis required for cellular survival. Two ENNs, ENN A and ENN B on hepatocarcinoma cells (HepG2) at 1.5 and 3 μM and three exposure times (24, 48 and 72 h) were studied. Flow cytometry was used to examine their effects on cell proliferation, to characterize at which phase of the cell cycle progression the cells were blocked and to study the role of the mitochondrial in ENNs-induced apoptosis. In conclusion, apoptosis induction on HepG2 cells allowed to compare cytotoxic effects caused by both ENNs, A and B. It is reported the possible mechanism observed in MMP changes, cell cycle analysis and apoptosis/necrosis, identifying ENN B more toxic than ENN A. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Biotransformation of the mycotoxin enniatin B1 in pigs: A comparative in vitro and in vivo approach.

Science.gov (United States)

Ivanova, Lada; Uhlig, Silvio; Devreese, Mathias; Croubels, Siska; Fæste, Christiane Kruse

2017-07-01

Enniatins (ENNs) are hexadepsipeptidic mycotoxins produced by Fusarium species. They occur in mg/kg levels in grain from Northern climate areas. Major ENNs such as ENN B and B1 have shown considerable cytotoxicity in different in vitro test systems. To adequately assess exposure and in vivo toxicity the toxicokinetic properties need to be investigated. The present study describes the metabolism of ENN B1 both in vitro and in vivo in pigs, comparing metabolites found in vitro in experiments with liver microsomes from different pig strains to those found in the plasma of pigs after single oral or intravenous application of ENN B1. Metabolites of hepatic biotransformation were tentatively identified and characterised by high performance liquid chromatography coupled to ion trap and high-resolution mass spectrometry, as well as chemical derivatisations. Kinetic parameters of metabolite formation and elimination were determined. Metabolite formation was higher when ENN B1 was absorbed from the gut compared to intravenous administration indicating pre-systemic metabolism of ENN B1 after oral uptake. The in vitro approach resulted in the detection of ten ENN B1 metabolites, while six were detected in in vivo samples. The putative ENN B1 metabolites were products of hydroxylation, carbonylation, carboxylation and oxidative demethylation reactions. Copyright © 2017. Published by Elsevier Ltd.

Adamkus lashes out at government, Cabinet

Index Scriptorium Estoniae

2005-01-01

Leedu president Valdas Adamkus pidas 12. juulil kõne, milles avaldas rahulolematust parlamendi tööga. Presidendi sõnul on parlament sügavas kriisis ning selmet töötada Leedu riigi ja rahva hüvanguks tegeleb see vaid iseenda eksistentsi kindlustamisega

Homme toimub NATO pilootidega ühisõppus

Index Scriptorium Estoniae

2010-01-01

Balti V õhuturbeharjutuse osana maanduvad Tallinna lennuväljal tankimiseks ja teeninduseks kaks prantsuse hävitajat Mirage 2000C, kaks poola hävitajat F-16, kaks Leedu reaktiivlennukit L-39 Albatross, Poola transpordilennuk CASA C-295M ja Leedu transpordilennuk Spartan C-27

Mockus goes to run-off in Colombian presidential election / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Kolumbias 30. mail toimunud presidendivalimistel sai Leedu juurtega Antanas Mockus 21% häältest, tema peamine vastane Juan Manuel Santos sai aga 47% häältest. Presidendivalimiste teine voor toimub 20. juunil. Poola presidendi Bronislav Komorowski ja Kreeka peaministri George Papandreu Leedu-juurtest

Mazheikiu Nafta - metalli väsimise või poliitika ohver / Tõnis Arnover

Index Scriptorium Estoniae

Arnover, Tõnis, 1952-

2006-01-01

Vene naftajuhtmemonopol Transneft sulges Leedu Mazeikiu Naftat varustava torujuhtme Sõprus-1 vahetult pärast seda, kui Vene Jukos ja Leedu valitsus olid sõlminud 84 protsendise Mazeikiu Nafta aktsiate müügi leppe Poola energiakontsernile PKN Orlen. Vt. samas: Vene-Poola jõukatsumine

Representing Lithuania's strongest ally / Anne E. Derse ; interviewed by Rokas M. Tracevskis

Index Scriptorium Estoniae

Derse, Anne E., 1954-

2011-01-01

USA suursaadik Leedus räägib, et USA kavatseb kontrollida Mihhail Golovatovi juhtumiga tegeleva komisjoni tööd, rõhutab, et president Barack Obama tahab investeerida haridusse ja et USA-Leedu suhetes on julgeolekul tähtis osa. Leedu kogukonnast Ameerika Ühendriikides

Enniatin B-induced cell death and inflammatory responses in RAW 267.4 murine macrophages

Energy Technology Data Exchange (ETDEWEB)

Gammelsrud, A. [Norwegian Veterinary Institute, P.O. Box 750, Centrum, N-0106 Oslo (Norway); Department of Air Pollution and Noise, Division of Environmental Medicine, Norwegian Institute of Public Health, N-0403 Oslo (Norway); Solhaug, A. [Norwegian Veterinary Institute, P.O. Box 750, Centrum, N-0106 Oslo (Norway); Dendelé, B. [EA 4427 SeRAIC, IRSET, Université de Rennes 1, IFR 140, Rennes (France); Sandberg, W.J. [Department of Air Pollution and Noise, Division of Environmental Medicine, Norwegian Institute of Public Health, N-0403 Oslo (Norway); Ivanova, L. [Norwegian Veterinary Institute, P.O. Box 750, Centrum, N-0106 Oslo (Norway); Kocbach Bølling, A. [Department of Air Pollution and Noise, Division of Environmental Medicine, Norwegian Institute of Public Health, N-0403 Oslo (Norway); Lagadic-Gossmann, D. [EA 4427 SeRAIC, IRSET, Université de Rennes 1, IFR 140, Rennes (France); Refsnes, M.; Becher, R. [Department of Air Pollution and Noise, Division of Environmental Medicine, Norwegian Institute of Public Health, N-0403 Oslo (Norway); Eriksen, G. [Norwegian Veterinary Institute, P.O. Box 750, Centrum, N-0106 Oslo (Norway); Holme, J.A., E-mail: jorn.holme@fhi.no [Department of Air Pollution and Noise, Division of Environmental Medicine, Norwegian Institute of Public Health, N-0403 Oslo (Norway)

2012-05-15

The mycotoxin enniatin B (EnnB) is predominantly produced by species of the Fusarium genera, and often found in grain. The cytotoxic effect of EnnB has been suggested to be related to its ability to form ionophores in cell membranes. The present study examines the effects of EnnB on cell death, differentiation, proliferation and pro-inflammatory responses in the murine monocyte–macrophage cell line RAW 264.7. Exposure to EnnB for 24 h caused an accumulation of cells in the G0/G1-phase with a corresponding decrease in cyclin D1. This cell cycle-arrest was possibly also linked to the reduced cellular ability to capture and internalize receptors as illustrated by the lipid marker ganglioside GM1. EnnB also increased the number of apoptotic, early apoptotic and necrotic cells, as well as cells with elongated spindle-like morphology. The Neutral Red assay indicated that EnnB induced lysosomal damage; supported by transmission electron microscopy (TEM) showing accumulation of lipids inside the lysosomes forming lamellar structures/myelin bodies. Enhanced levels of activated caspase-1 were observed after EnnB exposure and the caspase-1 specific inhibitor ZYVAD-FMK reduced EnnB-induced apoptosis. Moreover, EnnB increased the release of interleukin-1beta (IL-1β) in cells primed with lipopolysaccharide (LPS), and this response was reduced by both ZYVAD-FMK and the cathepsin B inhibitor CA-074Me. In conclusion, EnnB was found to induce cell cycle arrest, cell death and inflammation. Caspase-1 appeared to be involved in the apoptosis and release of IL-1β and possibly activation of the inflammasome through lysosomal damage and leakage of cathepsin B. -- Highlights: ► The mycotoxin EnnB induced cell cycle arrest, cell death and inflammation. ► The G0/G1-arrest was linked to a reduced ability to internalize receptors. ► EnnB caused lysosomal damage, leakage of cathepsin B and caspase-1 cleavage. ► Caspase-1 was partly involved in both apoptosis and release of IL-1�

Enniatin B-induced cell death and inflammatory responses in RAW 267.4 murine macrophages

International Nuclear Information System (INIS)

Gammelsrud, A.; Solhaug, A.; Dendelé, B.; Sandberg, W.J.; Ivanova, L.; Kocbach Bølling, A.; Lagadic-Gossmann, D.; Refsnes, M.; Becher, R.; Eriksen, G.; Holme, J.A.

2012-01-01

The mycotoxin enniatin B (EnnB) is predominantly produced by species of the Fusarium genera, and often found in grain. The cytotoxic effect of EnnB has been suggested to be related to its ability to form ionophores in cell membranes. The present study examines the effects of EnnB on cell death, differentiation, proliferation and pro-inflammatory responses in the murine monocyte–macrophage cell line RAW 264.7. Exposure to EnnB for 24 h caused an accumulation of cells in the G0/G1-phase with a corresponding decrease in cyclin D1. This cell cycle-arrest was possibly also linked to the reduced cellular ability to capture and internalize receptors as illustrated by the lipid marker ganglioside GM1. EnnB also increased the number of apoptotic, early apoptotic and necrotic cells, as well as cells with elongated spindle-like morphology. The Neutral Red assay indicated that EnnB induced lysosomal damage; supported by transmission electron microscopy (TEM) showing accumulation of lipids inside the lysosomes forming lamellar structures/myelin bodies. Enhanced levels of activated caspase-1 were observed after EnnB exposure and the caspase-1 specific inhibitor ZYVAD-FMK reduced EnnB-induced apoptosis. Moreover, EnnB increased the release of interleukin-1beta (IL-1β) in cells primed with lipopolysaccharide (LPS), and this response was reduced by both ZYVAD-FMK and the cathepsin B inhibitor CA-074Me. In conclusion, EnnB was found to induce cell cycle arrest, cell death and inflammation. Caspase-1 appeared to be involved in the apoptosis and release of IL-1β and possibly activation of the inflammasome through lysosomal damage and leakage of cathepsin B. -- Highlights: ► The mycotoxin EnnB induced cell cycle arrest, cell death and inflammation. ► The G0/G1-arrest was linked to a reduced ability to internalize receptors. ► EnnB caused lysosomal damage, leakage of cathepsin B and caspase-1 cleavage. ► Caspase-1 was partly involved in both apoptosis and release of IL-1�

AAV-Mediated Gene Targeting Is Significantly Enhanced by Transient Inhibition of Nonhomologous End Joining or the Proteasome In Vivo

Science.gov (United States)

Paulk, Nicole K.; Loza, Laura Marquez; Finegold, Milton J.

2012-01-01

Abstract Recombinant adeno-associated virus (rAAV) vectors have clear potential for use in gene targeting but low correction efficiencies remain the primary drawback. One approach to enhancing efficiency is a block of undesired repair pathways like nonhomologous end joining (NHEJ) to promote the use of homologous recombination. The natural product vanillin acts as a potent inhibitor of NHEJ by inhibiting DNA-dependent protein kinase (DNA-PK). Using a homology containing rAAV vector, we previously demonstrated in vivo gene repair frequencies of up to 0.1% in a model of liver disease hereditary tyrosinemia type I. To increase targeting frequencies, we administered vanillin in combination with rAAV. Gene targeting frequencies increased up to 10-fold over AAV alone, approaching 1%. Fah−/−Ku70−/− double knockout mice also had increased gene repair frequencies, genetically confirming the beneficial effects of blocking NHEJ. A second strategy, transient proteasomal inhibition, also increased gene-targeting frequencies but was not additive to NHEJ inhibition. This study establishes the benefit of transient NHEJ inhibition with vanillin, or proteasome blockage with bortezomib, for increasing hepatic gene targeting with rAAV. Functional metabolic correction of a clinically relevant disease model was demonstrated and provided evidence for the feasibility of gene targeting as a therapeutic strategy. PMID:22486314

Eesti kasiinofirma sattus Leedus skandaali keskele / Toomas Sildam, Rasmus Kagge

Index Scriptorium Estoniae

Sildam, Toomas, 1961-

2006-01-01

Leedu julgeolekuamet konfiskeeris ajalehe Laisvas Laikrastise kogu 8.septembri tiraazhi, väidetavalt kirjutati ajalehes julgeolekuameti poolt pealtkuulatud telefonikõnedest, millest ühes olevat Eesti suurärimees, Olympic Casino omanik Armin Karu nõudnud oma Vilniuse esindajatelt kokkusaamist temalt raha saanud Leedu sotsiaaldemokraatliku partei liidri Arturas Paulauskasega

Raud kureerib kunstnikke

Index Scriptorium Estoniae

2001-01-01

21. dets.-12. jaan. on on Haus galeriis Rein Raua kuraatoriprojekt "KevadSuviSügisTalv. Murdumispunkt". Näitusel osalevad Eve Kask, Peeter Laurits, Dainius Lishkevicius (Leedu), Rekha Menon (India/USA), Mall Nukke, Herkki Merila, Jorma Puranen (Soome), Rosita Raud, Ene-Liis Semper, Liina Siib, S&P Stanikas (Leedu)

Visiting Germany and Iceland / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Leedu president Dalia Grybauskaite külastas 24. augustil Saksamaad, kus kohtus kantsler Angela Merkeliga, kes tunnistati maailma võimekaimaks naiseks. 25.-27. augustil külastas Dalia Grybauskaite Islandit ja kohtus sealse riigipea Olafur Ragnar Grimssoniga, et tänada riiki Leedu 1991. a. taasiseseisvumise tunnustamise eest

Koostööleping kinnitab meie tahet / Krista Visas

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Visas, Krista

2015-01-01

Märtsis 2015 toimus Leedu väikelinnas Birštonases Eesti, Läti ja Leedu raamatukoguhoidjate ühingute juhatuste esindajate kohtumine, kus arutuse all oli Baltimaade ühingute kogemused-võimalused teha koostööd ja korraldada rahvusvahelisi kohtumisi. Ühistegevuse kinnituseks allkirjastati koostööleping

Algirdas Brazauskas lubab Leedule uusi paremaid radareid / Algirdas Brazauskas ; interv. Toomas Sildam

Index Scriptorium Estoniae

Brazauskas, Algirdas, 1932-2010

2005-01-01

Leedu peaminister Algirdas Brazauskas vastab küsimustele, mis puudutavad Vene hävitaja allakukkumist Kaunase lähedal, õhujõudude juhataja kolonel Jonas Marcinkuse vabastamist ning Leedu õhuruumi turbe piisavust ja parandamise vajalikkust. Lisa: Vene hävitaja Leedus. Vt. samas: Balti riigid tahavad Schengeni leppega liituda 2007. aastal

Detention of Star I Airline's only plane causes havoc / Linas Jegelevicius

Index Scriptorium Estoniae

Jegelevicius, Linas

2010-01-01

Leedu lennukompanii Star1 Airlines teatas 24. septembril, et peatab kõik oma lennud kuni 2. oktoobrini. Leedu lennuaamet uurib Star1 Airlinesi finantsseisu. Paljud reisijad on jäänud lennujaamadesse. Transpordiminister Eligijus Masiulise sõnul võidakse firmalt Euroopa Liidu reeglite rikkumise tõttu lennulitsents ära võtta

One more high ranking meeting with Putin / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Leedu peaministri Andrius Kubiliuse ja Venemaa peaministri Vladimir Putini kohtumisel Novo-Ogariovos räägiti majandussuhetest ning energeetikast. Nendel teemadel räägiti ka Leedu presidendi Dalia Grybauskaite ja Vladimir Putini 10. veebruaril Helsingis toimunud kohtumisel. Andrius Kubilius edastas Vladimir Putinile kutse osaleda 1.-2. juunil toimuval Balti assambleel

Обоюдоострая Марина / Рамуте Скучайте ; беседу ведет публицист Татьяна Ясинская

Index Scriptorium Estoniae

Скучайте, Рамуте, 1931-

2009-01-01

Leedu luuletaja ja tõlkija Ramutė Skučaitė oma elust väljasaadetuna Siberis 1949-1956. Ka on ta tõlkinud Marina Tsvetajeva loomingut leedu keelde, ilmunud on Tsvetajeva kakskeelne tõlkekogumik "Письма в никуда" (Vilnius : Kronta, 2008)

Intrathecal long-term gene expression by self-complementary adeno-associated virus type 1 suitable for chronic pain studies in rats

Directory of Open Access Journals (Sweden)

Janssen William GM

2006-01-01

Full Text Available Abstract Background Intrathecal (IT gene transfer is an attractive approach for targeting spinal mechanisms of nociception but the duration of gene expression achieved by reported methods is short (up to two weeks impairing their utility in the chronic pain setting. The overall goal of this study was to develop IT gene transfer yielding true long-term transgene expression defined as ≥ 3 mo following a single vector administration. We defined "IT" administration as atraumatic injection into the lumbar cerebrospinal fluid (CSF modeling a lumbar puncture. Our studies focused on recombinant adeno-associated virus (rAAV, one of the most promising vector types for clinical use. Results Conventional single stranded rAAV2 vectors performed poorly after IT delivery in rats. Pseudotyping of rAAV with capsids of serotypes 1, 3, and 5 was tested alone or in combination with a modification of the inverted terminal repeat. The former alters vector tropism and the latter allows packaging of self-complementary rAAV (sc-rAAV vectors. Combining both types of modification led to the identification of sc-rAAV2/l as a vector that performed superiorly in the IT space. IT delivery of 3 × 10e9 sc-rAAV2/l particles per animal led to stable expression of enhanced green fluorescent protein (EGFP for ≥ 3 mo detectable by Western blotting, quantitative PCR, and in a blinded study by confocal microscopy. Expression was strongest in the cauda equina and the lower sections of the spinal cord and only minimal in the forebrain. Microscopic examination of the SC fixed in situ with intact nerve roots and meninges revealed strong EGFP fluorescence in the nerve roots. Conclusion sc-rAAVl mediates stable IT transgene expression for ≥ 3 mo. Our findings support the underlying hypothesis that IT target cells for gene transfer lack the machinery for efficient conversion of the single-stranded rAAV genome into double-stranded DNA and favor uptake of serotype 1 vectors over 2

Baltic Democracy Exporters to Central Asia? A Lithuanian Perspective / Egdunas Racius

Index Scriptorium Estoniae

Racius, Egdunas

2006-01-01

Demokraatliku ühiskonna staatuse omandanud ning liberaalse majandusmudeliga Leedu on moraalselt kohustatud jagama oma kogemusi vähem edukate riikidega. Kuid peab olema realistlik, kus ja mida on võimalik saavutada. Senini on Leedu poliitiline eliit hoidunud "demokraatia levitamisest" Kesk-Aasiasse ning on keskendunud geograafiliselt lähedalolevatele endistele nõukogude vabariikidele

Sõprusest ja armastusest / Kristi Paap

Index Scriptorium Estoniae

Paap, Kristi

2011-01-01

Eesti, läti ja leedu keraamikanäitus "Družba narodov ehk rahvaste sõprus" Eesti Tarbekunsti- ja Disainimuuseumis 10. aprillini 2011. 15 osalejat loetletud. Domilė Ragauskaitė (Leedu), Tõnis Kriisa, Ingrid Alliku ja Tiina Kaljuste töödest. Leo Rohlini fragmentaarium " Nokturn" Tallinnas galeriis Hop 22. veebr. 2011

Baltic region going nuclear

Index Scriptorium Estoniae

2011-01-01

Läti keskkonnaminister Raimonds Vejonis leiab, et Läti ei peaks investeerima Leetu rajatava tuumaelektrijaama ehitusse ja võiks panustada hoopis taastuvenergiale. Venemaa peaminister Vladimir Putin ja Valgevene president Aleksandr Lukašenko allkirjastasid kokkuleppe Valgevene ja Leedu piiri lähistele uue tuumajaama ehitamise kohta. Leedu ei ole selle projektiga nõus

Heated squabble over Belarus marks beginning of the year / Linas Jegelevicius

Index Scriptorium Estoniae

Jegelevicius, Linas

2011-01-01

Leedu ekspresident Valdas Adamkus on kritiseerinud president Dalia Grybauskaite välispoliitikat Valgevene suhtes. 3. jaanuaril toimunud presidendi ja välisminister Andronius Azubalise kohtumisel räägiti ka Valgevene presidendivalimistest ja sealsest olukorrast. Dalia Grybauskaite prognoose ja eesmärke 2011. aastaks on samuti kritiseeritud. Leedu on 2011. aastal OSCE eesistuja

Reflections on "Twenty Years of Feminist Counseling and Therapy."

Science.gov (United States)

Rawlings, Edna I.

1993-01-01

Responds to previous article by Carolyn Zerbe Enns on feminist counseling and psychotherapy. Sees Enns's article as a valuable resource and reacts to Enns's article from the perspective of a radical feminist therapist. Discusses the difficulty involved in trying to effectively integrate radical feminism with psychotherapy. (NB)

Vahepõlvkonna idealiseeritud mälestused / Ernest Truely ; tõlkinud Ulla Juske

Index Scriptorium Estoniae

Truely, Ernest

2011-01-01

Fotokuu näitus "Sündinud samas kohas: kujutis, mälu ja fiktsioon Baltimaades" Tallinna Kunstihoones 20. nov.-ni 2011. Kuraator Vytautas Michelkevičius (Leedu), kujundaja Dénes Farkas. Sigrid Viiri, Marge Monko (Eesti), Tadas Šarūnase (Leedu), Kaspars Podnieksi ja Arnis Balčuse (Läti) töödest

Obama reassured Kubilius about U.S. security guarantees / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Ameerika Ühendriikide president Barack Obama ja Venemaa riigipea kohtusid 8. aprillil Prahas ning allkirjastasid uue tuumarelvastuse piiramise leppe. Barack Obama oli Prahasse lõunale kutsunud Eesti, Läti, Leedu ja Rumeenia presidendid ning Poola, Tšehhi, Bulgaaria, Horvaatia, Ungari, Slovakkia ja Sloveenia peaministrid. Leedu president Dalia Grybauskaite loobus kutsest, teda asendas peaminister Andrius Kubilius

The Third Decade of Feminist Therapy and the Personal Is Still Political.

Science.gov (United States)

Gilbert, Lucia Albino; Rossman, Karen M.

1993-01-01

Responds to earlier article by Carolyn Zerbe Enns on feminist counseling and psychotherapy. Asserts that Enns presents concise summary and overview but offers little beyond her examination of "themes of change, agreement, and disagreement." Focuses comments on two topics addressed by Enns: the importance of context and interrelationships…

A Review of the Mycotoxin Enniatin B

Directory of Open Access Journals (Sweden)

Alessandra Prosperini

2017-11-01

Full Text Available Mycotoxin enniatin B (ENN B is a secondary metabolism product by Fusarium fungi. It is a well-known antibacterial, antihelmintic, antifungal, herbicidal, and insecticidal compound. It has been found as a contaminant in several food commodities, particularly in cereal grains, co-occurring also with other mycotoxins. The primary mechanism of action of ENN B is mainly due to its ionophoric characteristics, but the exact mechanism is still unclear. In the last two decades, it has been a topic of great interest since its potent mammalian cytotoxic activity was demonstrated in several mammalian cell lines. Moreover, the co-exposure in vitro with other mycotoxins enhances its toxic potential through synergic effects, depending on the concentrations tested. Despite its clear cytotoxic effect, European Food Safety Authority stated that acute exposure to ENNs, such as ENN B, does not indicate concern for human health, but a concern might be the chronic exposure. However, given the lack of relevant toxicity data, no firm conclusion could be drawn and a risk assessment was not possible. In fact, very few studies have been carried out in vivo and, in these studies, no adverse effects were observed. So, research on toxicological effects induced by ENN B is still on-going. Recently, some studies are dealing with new advances regarding ENN B. This review summarizes the information on biochemical and biological activity of ENN B, focusing on toxicological aspects and on the latest advances in research on ENN B.

Baltic Sea oil spill may be inevitable / Linas Jegelevicius

Index Scriptorium Estoniae

Jegelevicius, Linas

2011-01-01

23.-24. märtsil Budapestis toimunud EL-i keskkonnanõukogu konverentsil märgiti, et Läänemere maad peavad olema võimalikuks naftareostuse tekkeks valmis ja räägiti ka Jaapanis juhtunud tuumakatastroofist. EL-i keskkonnavolinik Janez Potocnik leiab, et Valgevene peaks oma Leedu piiri lähedale plaanitava tuumaelektrijaama küsimuses ka Leedu huvidega arvestama

Cido fruit juice entangled in quality scare

Index Scriptorium Estoniae

2005-01-01

Leedu Veterinaar- ja Toidulabor avastas Lätis toodetud Cido greibimahlakontsentraadist 210 mg/kg mürkainet, mis on üle nelja korra lubatud määrast rohkem mürkainet. Cido turundusdirektori Dmitrijs Tairovsi sõnul leiti Lätis ja Saksamaal tehtud testide põhjal ainet tunduv alt vähem. Ettevõtte tegevdirektor Vadims Vlasovs kahtlustab Leedu konkurente

Grybauskaite rejects Obama's invitation / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

8. aprillil kirjutavad Ameerika Ühendriikide president Barack Obama ja Venemaa president Dmitri Medvedev Prahas uuele strateegilise tuumarelvastuse piiramise leppele alla. Samaks päevaks on Barack Obama Prahasse kutsunud Eesti, Läti, Leedu ja Rumeenia presidendid ning Poola, Tšehhi, Bulgaaria, Horvaatia, Ungari, Slovakkia ja Sloveenia peaministrid. Leedu president Dalia Grybauske loobus kutsest. Tema asemel sõidab kohtumisele peaminister Andrius Kubilius

Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR

Directory of Open Access Journals (Sweden)

Susan D'Costa

2016-01-01

Full Text Available Clinical trials using recombinant adeno-associated virus (rAAV vectors have demonstrated efficacy and a good safety profile. Although the field is advancing quickly, vector analytics and harmonization of dosage units are still a limitation for commercialization. AAV reference standard materials (RSMs can help ensure product safety by controlling the consistency of assays used to characterize rAAV stocks. The most widely utilized unit of vector dosing is based on the encapsidated vector genome. Quantitative polymerase chain reaction (qPCR is now the most common method to titer vector genomes (vg; however, significant inter- and intralaboratory variations have been documented using this technique. Here, RSMs and rAAV stocks were titered on the basis of an inverted terminal repeats (ITRs sequence-specific qPCR and we found an artificial increase in vg titers using a widely utilized approach. The PCR error was introduced by using single-cut linearized plasmid as the standard curve. This bias was eliminated using plasmid standards linearized just outside the ITR region on each end to facilitate the melting of the palindromic ITR sequences during PCR. This new “Free-ITR” qPCR delivers vg titers that are consistent with titers obtained with transgene-specific qPCR and could be used to normalize in-house product-specific AAV vector standards and controls to the rAAV RSMs. The free-ITR method, including well-characterized controls, will help to calibrate doses to compare preclinical and clinical data in the field.

Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR.

Science.gov (United States)

D'Costa, Susan; Blouin, Veronique; Broucque, Frederic; Penaud-Budloo, Magalie; François, Achille; Perez, Irene C; Le Bec, Christine; Moullier, Philippe; Snyder, Richard O; Ayuso, Eduard

2016-01-01

Clinical trials using recombinant adeno-associated virus (rAAV) vectors have demonstrated efficacy and a good safety profile. Although the field is advancing quickly, vector analytics and harmonization of dosage units are still a limitation for commercialization. AAV reference standard materials (RSMs) can help ensure product safety by controlling the consistency of assays used to characterize rAAV stocks. The most widely utilized unit of vector dosing is based on the encapsidated vector genome. Quantitative polymerase chain reaction (qPCR) is now the most common method to titer vector genomes (vg); however, significant inter- and intralaboratory variations have been documented using this technique. Here, RSMs and rAAV stocks were titered on the basis of an inverted terminal repeats (ITRs) sequence-specific qPCR and we found an artificial increase in vg titers using a widely utilized approach. The PCR error was introduced by using single-cut linearized plasmid as the standard curve. This bias was eliminated using plasmid standards linearized just outside the ITR region on each end to facilitate the melting of the palindromic ITR sequences during PCR. This new "Free-ITR" qPCR delivers vg titers that are consistent with titers obtained with transgene-specific qPCR and could be used to normalize in-house product-specific AAV vector standards and controls to the rAAV RSMs. The free-ITR method, including well-characterized controls, will help to calibrate doses to compare preclinical and clinical data in the field.

Assessment of tropism and effectiveness of new primate-derived hybrid recombinant AAV serotypes in the mouse and primate retina.

Directory of Open Access Journals (Sweden)

Peter Charbel Issa

Full Text Available Adeno-associated viral vectors (AAV have been shown to be safe in the treatment of retinal degenerations in clinical trials. Thus, improving the efficiency of viral gene delivery has become increasingly important to increase the success of clinical trials. In this study, structural domains of different rAAV serotypes isolated from primate brain were combined to create novel hybrid recombinant AAV serotypes, rAAV2/rec2 and rAAV2/rec3. The efficacy of these novel serotypes were assessed in wild type mice and in two models of retinal degeneration (the Abca4(-/- mouse which is a model for Stargardt disease and in the Pde6b(rd1/rd1 mouse in vivo, in primate tissue ex-vivo, and in the human-derived SH-SY5Y cell line, using an identical AAV2 expression cassette. We show that these novel hybrid serotypes can transduce retinal tissue in mice and primates efficiently, although no more than AAV2/2 and rAAV2/5 serotypes. Transduction efficiency appeared lower in the Abca4(-/- mouse compared to wild type with all vectors tested, suggesting an effect of specific retinal diseases on the efficiency of gene delivery. Shuffling of AAV capsid domains may have clinical applications for patients who develop T-cell immune responses following AAV gene therapy, as specific peptide antigen sequences could be substituted using this technique prior to vector re-treatments.

Assessment of tropism and effectiveness of new primate-derived hybrid recombinant AAV serotypes in the mouse and primate retina.

Science.gov (United States)

Charbel Issa, Peter; De Silva, Samantha R; Lipinski, Daniel M; Singh, Mandeep S; Mouravlev, Alexandre; You, Qisheng; Barnard, Alun R; Hankins, Mark W; During, Matthew J; Maclaren, Robert E

2013-01-01

Adeno-associated viral vectors (AAV) have been shown to be safe in the treatment of retinal degenerations in clinical trials. Thus, improving the efficiency of viral gene delivery has become increasingly important to increase the success of clinical trials. In this study, structural domains of different rAAV serotypes isolated from primate brain were combined to create novel hybrid recombinant AAV serotypes, rAAV2/rec2 and rAAV2/rec3. The efficacy of these novel serotypes were assessed in wild type mice and in two models of retinal degeneration (the Abca4(-/-) mouse which is a model for Stargardt disease and in the Pde6b(rd1/rd1) mouse) in vivo, in primate tissue ex-vivo, and in the human-derived SH-SY5Y cell line, using an identical AAV2 expression cassette. We show that these novel hybrid serotypes can transduce retinal tissue in mice and primates efficiently, although no more than AAV2/2 and rAAV2/5 serotypes. Transduction efficiency appeared lower in the Abca4(-/-) mouse compared to wild type with all vectors tested, suggesting an effect of specific retinal diseases on the efficiency of gene delivery. Shuffling of AAV capsid domains may have clinical applications for patients who develop T-cell immune responses following AAV gene therapy, as specific peptide antigen sequences could be substituted using this technique prior to vector re-treatments.

Recombinant AAV-mediated in vivo long-term expression and antitumour activity of an anti-ganglioside GM3(Neu5Gc) antibody.

Science.gov (United States)

Piperno, G M; López-Requena, A; Predonzani, A; Dorvignit, D; Labrada, M; Zentilin, L; Burrone, O R; Cesco-Gaspere, M

2015-12-01

The ganglioside GM3(Neu5Gc) has gained increasing attention as therapeutic target because of its selective expression in various human tumours, such as melanoma, breast and lung cancer. 14F7 is a mouse IgG1 with specific reactivity to GM3(Neu5Gc)-positive tumours. The therapeutic activity of 14F7 has also been demonstrated in vivo, through its repetitive passive administration in tumour-bearing animals. In this work we used an alternative strategy to deliver recombinant 14F7 in vivo and analysed the therapeutic efficacy of this approach. We engineered a recombinant adeno-associated vector to direct the expression of secretable recombinant 14F7 in BALB/c animals. A single administration of the rAAV induced efficient production and secretion of the antibody in the bloodstream, with an expression level reaching plateau at ∼3 weeks after injection and persisting for almost a year. Strikingly, upon challenge with GM3(Neu5Gc)-positive X63-AG8.653 myeloma cells, tumour development was significantly delayed in animals treated with rAAV-14F7 with respect to animals treated with a control rAAV codifying for an irrelevant antibody. Finally, no significant differences in survival proportion were detected in animals injected with rAAV-14F7 or treated by standard administration of repetitive doses of purified monoclonal antibody 14F7.

L’indépendance d’une Autorité Régulatrice Nationale (ARN créée selon la législation européenne dans le secteur des communications électroniques – L’exemple Grec

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Vassilios Theodoros Kondylis

2017-07-01

Full Text Available Cette étude se penche sur la question de l'indépendance dans le statut juridique d'une autorité de régulation nationale (ARN du secteur des communications électroniques. La première partie analyse le fondement juridique de l'indépendance et le contenu de ce concept en tenant compte de l'évolution récente de la jurisprudence de la Cour de justice de l’Union européenne (CJUE. La deuxième partie examine les mesures adoptées par le Législateur grec afin d'assurer l'indépendance de l’ARN, les compétences de cette dernière et les résultats de ces mesures au vu de la garantie de l'ouverture des marchés en cause à la libre concurrence.

Long-term gene therapy causes transgene-specific changes in the morphology of regenerating retinal ganglion cells.

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Jennifer Rodger

Full Text Available Recombinant adeno-associated viral (rAAV vectors can be used to introduce neurotrophic genes into injured CNS neurons, promoting survival and axonal regeneration. Gene therapy holds much promise for the treatment of neurotrauma and neurodegenerative diseases; however, neurotrophic factors are known to alter dendritic architecture, and thus we set out to determine whether such transgenes also change the morphology of transduced neurons. We compared changes in dendritic morphology of regenerating adult rat retinal ganglion cells (RGCs after long-term transduction with rAAV2 encoding: (i green fluorescent protein (GFP, or (ii bi-cistronic vectors encoding GFP and ciliary neurotrophic factor (CNTF, brain-derived neurotrophic factor (BDNF or growth-associated protein-43 (GAP43. To enhance regeneration, rats received an autologous peripheral nerve graft onto the cut optic nerve of each rAAV2 injected eye. After 5-8 months, RGCs with regenerated axons were retrogradely labeled with fluorogold (FG. Live retinal wholemounts were prepared and GFP positive (transduced or GFP negative (non-transduced RGCs injected iontophoretically with 2% lucifer yellow. Dendritic morphology was analyzed using Neurolucida software. Significant changes in dendritic architecture were found, in both transduced and non-transduced populations. Multivariate analysis revealed that transgenic BDNF increased dendritic field area whereas GAP43 increased dendritic complexity. CNTF decreased complexity but only in a subset of RGCs. Sholl analysis showed changes in dendritic branching in rAAV2-BDNF-GFP and rAAV2-CNTF-GFP groups and the proportion of FG positive RGCs with aberrant morphology tripled in these groups compared to controls. RGCs in all transgene groups displayed abnormal stratification. Thus in addition to promoting cell survival and axonal regeneration, vector-mediated expression of neurotrophic factors has measurable, gene-specific effects on the morphology of injured

Poland wants more EU cooperation with Baltic States / Arturas Racas

Index Scriptorium Estoniae

Racas, Arturas

2006-01-01

Balti riikide ja Poola presidendid kohtusid Vilniuses. Teemadeks olid: NATO, Euroopa Liidu, Gruusia küsimused, koostöö tuumaelektrijaama rajamisel, vastastikuste suhete tihendamine. Vt. samas lk. 12: Presidents bond over ambitious communique. Läti, Leedu, Eesti ja Poola presidendid kirjutasid alla kommunikeele regionaalse koostöö tugevdamiseks, rõhuga infrastruktuuri ja energia küsimustel. Vabariigi President töövisiidil Leedu Vabariiki 6.10.2006

Physical activity patterns in the French 18-74-year-old population: French Nutrition and Health Survey (Etude Nationale Nutrition Santé, ENNS) 2006-2007.

Science.gov (United States)

Salanave, Benoit; Vernay, Michel; Szego, Emmanuelle; Malon, Aurélie; Deschamps, Valérie; Hercberg, Serge; Castetbon, Katia

2012-11-01

To describe detailed physical activity and sedentary behaviour in French adults across physical activity categories. The French Nutrition and Health Survey (Etude Nationale Nutrition Santé, ENNS), conducted in 2006-2007, was a national cross-sectional survey based on three-stage random sampling. The International Physical Activity Questionnaire (IPAQ) was used to classify participants into three physical activity categories. Time spent in a sitting position and time spent in front of a screen were used as markers of sedentary behaviour. France. Adults (n 2971) aged 18 to 74 years were included. Overall, 29·5 % of men and 23·6 % of women were classified into the high-IPAQ category, while 36·1 % of men and 37·5 % of women were in the low-IPAQ category. For each intensity level of physical activity (vigorous intensity, moderate intensity or walking), the number of active days per week decreased from the high- to the low-IPAQ category and daily duration of physical activity was longer in the high-IPAQ category than in the other two categories; 6 % of adults declared neither vigorous nor moderate nor walking activities. For most adults in the low-IPAQ category, an increasing number of active days per week would be sufficient to attain the moderate-IPAQ category. This should be taken into account in public health initiatives aimed at promoting physical activity.

A translational approach for limb vascular delivery of the micro-dystrophin gene without high volume or high pressure for treatment of Duchenne muscular dystrophy

Directory of Open Access Journals (Sweden)

Chicoine Louis G

2007-09-01

Full Text Available Abstract Background Duchenne muscular dystrophy (DMD is an X-linked recessive disorder with monogenic mutations setting the stage for successful gene therapy treatment. We have completed a study that directly deals with the following key issues that can be directly adapted to a gene therapy clinical trial using rAAV considering the following criteria: 1 A regional vascular delivery approach that will protect the patient from widespread dissemination of virus; 2 an approach to potentially facilitate safe passage of the virus for efficient skeletal muscle transduction; 3 the use of viral doses to accommodate current limitations imposed by vector production methods; 4 and at the same time, achieve a clinically meaningful outcome by transducing multiple muscles in the lower limb to prolong ambulation. Methods The capacity of AAV1, AAV6 or AAV8 to cross the vascular endothelial barrier carrying a micro-dystrophin cDNA was compared under identical conditions with delivery through a catheter placed in the femoral artery of the mdx mouse. Transduction efficiency was assessed by immuno-staining using an antibody (Manex1a that recognizes the N-terminus of micro-dystrophin. The degree of physiologic correction was assessed by measuring tetanic force and protection from eccentric contraction in the extensor digitorum longus muscle (EDL. The vascular delivery paradigm found successful in the mouse was carried to the non-human primate to test its potential translation to boys with DMD. Results Regional vascular delivery resulted in transduction by rAAV8.micro-dystrophin reaching 94.5 ± 0.9 (1 month, 91.3 ± 3.1 (2 months, and 89.6 ± 1.6% (3 months. rAAV6.micro-dystrophin treated animals demonstrated 87.7 ± 6.8 (1 month, 78.9 ± 7.4 (2 months, and 81.2 ± 6.2% (3 months transduction. In striking contrast, rAAV1 demonstrated very low transduction efficiency [0.9 ± 0.3 (1 month, 2.1 ± 0.8 (2 months, and 2.1 ± 0.7% (3 months] by vascular delivery. Micro

Adeno-associated Virus Serotype 9 - Driven Expression of BAG3 Improves Left Ventricular Function in Murine Hearts with Left Ventricular Dysfunction Secondary to a Myocardial Infarction.

Science.gov (United States)

Knezevic, Tijana; Myers, Valerie D; Su, Feifei; Wang, JuFang; Song, Jianliang; Zhang, Xue-Qian; Gao, Erhe; Gao, Guofeng; Muniswamy, Madesh; Gupta, Manish K; Gordon, Jennifer; Weiner, Kristen N; Rabinowitz, Joseph; Ramsey, Frederick V; Tilley, Douglas G; Khalili, Kamel; Cheung, Joseph Y; Feldman, Arthur M

2016-12-01

The present study was undertaken to test the hypothesis that gene delivery of BCL2-Associated Athanogene 3 (BAG3) to the heart of mice with left ventricular dysfunction secondary to a myocardial infarction could enhance cardiac performance. BAG3 is a 575 amino acid protein that has pleotropic functions in the cell including pro-autophagy and anti-apoptosis. Mutations in BAG3 have been associated with both skeletal muscle dysfunction and familial dilated cardiomyopathy and BAG3 levels are diminished in non-familial heart failure. Eight-week-old C57/BL6 mice underwent ligation of the left coronary artery (MI) or sham surgery (Sham). Eight weeks later, mice in both groups were randomly assigned to receive either a retro-orbital injection of rAAV9-BAG3 (MI-BAG3 or Sham-BAG3) or rAAV9-GFP (MI-GFP or Sham GFP). Mice were sacrificed at 3 weeks post-injection and myocytes were isolated from the left ventricle. MI-BAG3 mice demonstrated a significantly (p BAG3 injection with further improvement in LVEF, fractional shortening and stroke volume at 3 weeks post-injection without changes in LV mass or LV volume. Injection of rAAV9-BAG3 had no effect on LVEF in Sham mice. The salutary benefits of rAAV9-BAG3 were also observed in myocytes isolated from MI hearts including improved cell shortening (pBAG3 gene therapy may provide a novel therapeutic option for the treatment of heart failure.

Adeno-associated Virus Serotype 9 – Driven Expression of BAG3 Improves Left Ventricular Function in Murine Hearts with Left Ventricular Dysfunction Secondary to a Myocardial Infarction

Science.gov (United States)

Knezevic, Tijana; Myers, Valerie D.; Su, Feifei; Wang, JuFang; Song, Jianliang; Zhang, Xue-Qian; Gao, Erhe; Gao, Guofeng; Muniswamy, Madesh; Gupta, Manish K.; Gordon, Jennifer; Weiner, Kristen N.; Rabinowitz, Joseph; Ramsey, Frederick V.; Tilley, Douglas G.; Khalili, Kamel; Cheung, Joseph Y.; Feldman, Arthur M.

2016-01-01

Objectives The present study was undertaken to test the hypothesis that gene delivery of BCL2-Associated Athanogene 3 (BAG3) to the heart of mice with left ventricular dysfunction secondary to a myocardial infarction could enhance cardiac performance. Background BAG3 is a 575 amino acid protein that has pleotropic functions in the cell including pro-autophagy and anti-apoptosis. Mutations in BAG3 have been associated with both skeletal muscle dysfunction and familial dilated cardiomyopathy and BAG3 levels are diminished in non-familial heart failure. Methods Eight-week-old C57/BL6 mice underwent ligation of the left coronary artery (MI) or sham surgery (Sham). Eight weeks later, mice in both groups were randomly assigned to receive either a retro-orbital injection of rAAV9-BAG3 (MI-BAG3 or Sham-BAG3) or rAAV9-GFP (MI-GFP or Sham GFP). Mice were sacrificed at 3 weeks post-injection and myocytes were isolated from the left ventricle. Results MI-BAG3 mice demonstrated a significantly (p BAG3 injection with further improvement in LVEF, fractional shortening and stroke volume at 3 weeks post-injection without changes in LV mass or LV volume. Injection of rAAV9-BAG3 had no effect on LVEF in Sham mice. The salutary benefits of rAAV9-BAG3 were also observed in myocytes isolated from MI hearts including improved cell shortening (pBAG3 gene therapy may provide a novel therapeutic option for the treatment of heart failure. PMID:28164169

Restoration of central nervous system alpha-N-acetylglucosaminidase activity and therapeutic benefits in mucopolysaccharidosis IIIB mice by a single intracisternal recombinant adeno-associated viral type 2 vector delivery.

Science.gov (United States)

Fu, Haiyan; DiRosario, Julianne; Kang, Lu; Muenzer, Joseph; McCarty, Douglas M

2010-07-01

Finding efficient central nervous system (CNS) delivery approaches has been the major challenge facing therapeutic development for treating diseases with global neurological manifestation, such as mucopolysaccharidosis (MPS) IIIB, a lysosomal storage disease, caused by autosomal recessive defect of alpha-N-acetylglucosaminidase (NaGlu). Previously, we developed an approach, intracisternal (i.c.) injection, to deliver recombinant adeno-associated viral (rAAV) vector to the CNS of mice, leading to a widespread periventricular distribution of transduction. In the present study, we delivered rAAV2 vector expressing human NaGlu into the CNS of MPS IIIB mice by an i.c. injection approach, to test its therapeutic efficacy and feasibility for treating the neurological manifestation of the disease. We demonstrated significant functional neurological benefits of a single i.c. vector infusion in adult MPS IIIB mice. The treatment slowed the disease progression by mediating widespread recombinant NaGlu expression in the CNS, resulting in the reduction of brain lysosomal storage pathology, significantly improved cognitive function and prolonged survival. However, persisting motor function deficits suggested that pathology in areas outside the CNS contributes to the MPS IIIB behavioral phenotype. The therapeutic benefit of i.c. rAAV2 delivery was dose-dependent and could be attribute solely to the CNS transduction because the procedure did not lead to detectable transduction in somatic tissues. A single IC rAAV2 gene delivery is functionally beneficial for treating the CNS disease of MPS IIIB in mice. It is immediately clinically translatable, with the potential of improving the quality of life for patients with MPS IIIB.

VP Market Eesti tööseadustega pahuksis / Merike Mikk

Index Scriptorium Estoniae

Mikk, Merike, 1973-

2004-01-01

Leedu jaekaubanduskontserni VP Market endiste töötajate sõnul on ettevõte rikkunud Eestis kehtivaid tööseadusi töölepingute sõlmimisel, mille tõttu on Tartu ja Tallinna töövaidluskomisjonidele esitatud lahendamiseks 14 avaldust. Vt. samas: Leedu poekett kasvab jõuliselt; T-Marketid saanud kaks ettekirjutust; VP Marketi kommentaar artiklile; Säästumarketis lisatasu kord kirjas; Intervjuu endise T-Marketi juhataja Tessa Kütimetsaga

Eesti ajalugu Vene-Liivimaa sõjast Põhjasõjani / Lea Kõiv

Index Scriptorium Estoniae

Kõiv, Lea, 1961-

2014-01-01

Arvustus: Enn Küng, Margus Laidre, Ivar Leimus, Aivar Põldvee, Anti Selart, Marten Seppel, Kai Tafenau, Ülle Tarkiainen, Enn Tarvel. Eesti ajalugu III: Vene-Liivimaa sõjast Põhjasõjani. Tartu, 2013

Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells

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Dalle-Donne Isabella

2008-10-01

Full Text Available Abstract Background Recent studies demonstrate that recombinant adeno-associated virus (rAAV-based antigen loading of dendritic cells (DCs generates in vitro, significant and rapid cytotoxic T-lymphocyte (CTL responses against viral antigens. Methods We used the rAAV system to induce specific CTLs against CVM antigens for the development of cytomegalovirus HCMV gene therapy. As an extension of the versatility of the rAAV system, we incorporated immediate-early 1 (IE1, expressed in HCMV. Our rAAV vector induced a strong stimulation of CTLs directed against the HCMV antigen IE1. We then investigated the efficiency of the CTLs in killing IE1 targeted cells. Results A significant MHC Class I-restricted, anti-IE1-specific CTL killing was demonstrated against IE1 positive peripheral blood mononuclear cells (PBMC after one, in vitro, stimulation. Conclusion In summary, single PBMC stimulation with rAAV/IE1 pulsed DCs induces strong antigen specific-CTL generation. CTLs were capable to lyse low doses of peptides pulsed into target cells. These data suggest that AAV-based antigen loading of DCs is highly effective for generating human CTL responses against HCMV antigens.

smport nt rust l growth in the h nerozoi X ssotopi eviden e of gr ...

Indian Academy of Sciences (India)

smport nt rust l growth in the h nerozoi X ssotopi eviden e of gr nitoids from e stE entr l esi fy Ewsxq terx. ID p ex. IDP nd he is ryxqQ. Iq eos ien es ennesD niversit e de ennes ID QSHRP ennes gedexD pr n e em ilX j hndunivErennesIFfr. Phep rtment of qeologyD gh ng hun niversity of ien e nd e hnologyD gh ng hun IQH ...

Mycotoxin occurrence on baled and pit silages collected in Co. Meath

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McElhinney C.

2015-12-01

Full Text Available Recent studies of baled silages produced in Ireland have identified considerable filamentous fungal contamination. Many of these fungi are toxigenic, capable of producing secondary metabolites, namely mycotoxins. Mycotoxins are potentially detrimental to livestock health and some can pose a risk to consumers of animal products. Baled (n=20 and pit (n=18 silages from a sample of farms (n=38 in Co. Meath were examined to assess the occurrence of mycotoxins and ascertain whether sampling position within the pit silos (feed face vs. 3 m behind the feed face has an effect on mycotoxin content or other chemical compositional variables. Of the 20 mycotoxins assayed, baled silages contained [mean of positive values (no. of values in mean] mycotoxin concentrations (μg/kg dry matter of beauvericin 36 (2, enniatin (enn. A 9.3 (3, enn. A1 54 (8, enn. B 351 (9, enn. B1 136 (10, mycophenolic acid (MPA 11,157 (8 and roquefortine C (Roq. C 1037 (8 and pit silages contained beauvericin 25 (2 enn. A1 18 (2, enn. B 194 (9, enn. B1 57 (3, MPA 287 (6, Roq. C 3649 (6 and zearalenone 76 (1. There was no difference (P>0.05 observed in the mycotoxin concentrations between baled and pit silages, and 11 of the 20 mycotoxins assayed were below the limits of detection. The position of sampling had no effect on the mycotoxin concentration detected in pit silages. It is concluded that mycotoxin concentrations detected in these pit and baled silages in Co. Meath did not exceed EU regulation or guidance limits, and that similar chemical composition and mycotoxin concentration values occurred at the pit silage feed face and 3 m behind this feed face.

Biodiesel : la réglementation européenne et le débat sur son évolution

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Dusser Philippe

2013-09-01

Full Text Available La réglementation communautaire sur les biocarburants a été mise en place à partir de 2003 pour répondre à un triple objectif : réduction des émissions de gaz à effet de serre, réduction de la dépendance énergétique, et soutien à la production agricole dans un contexte où 10 % des terres étaient obligatoirement gelées. Cette réglementation a été renforcée en 2009 dans le cadre du « paquet énergie » par l’introduction par la directive energie renouvelable d’un objectif obligatoire d’incorporation de 10 % d’énergie renouvelable dans les carburants à l’horizon 2020. Cette législation est complétée par une directive qualité des carburants qui impose une réduction de 6 % d’ici 2020 de l’empreinte carbone des carburants. La forte diésélisation du parc de véhicule en Europe a conduit à une rapide progression de la consommation de biodiesel (11 millions de tonnes dont 9 produites dans l’UE. L’huile de colza est la principale matière première du biodiesel. L’augmentation de la production de colza a permis de répondre à cette demande, tout en fournissant un coproduit protéique permettant de réduire significativement le déficit en protéines européen. Le débat sur l’impact du CASI (changement indirect d’affectation des sols des biocarburants, ainsi que celui sur l’équilibre alimentaire, a conduit la Commission européenne, 3 ans après avoir défini sa politique, à proposer de plafonner les carburants issus des cultures (1ere génération à 5 % et à introduire des « facteurs CASI » dans l’évaluation des effets de réduction des gaz à effets de serre. Cette proposition qui pourrait fortement remettre en cause le développement du biodiesel...et de la culture de colza fait à la date de rédaction de cet article (juillet 2013 l’objet de difficiles négociations à Bruxelles, dont l’issue est difficile à prédire.

[Eesti ajalugu III: Vene-Liivimaa sõjast Põhjasõjani] / Kalle Kroon

Index Scriptorium Estoniae

Kroon, Kalle, 1966-

2014-01-01

Arvustus. Enn Küng, Margus Laidre, Ivar Leimus, Aivar Põldvee, Anti Selart, Marten Sepepl, Kai Tafenau, Ülle Tarkiainen, Enn Tarvel. Eesti ajalugu III: Vene-Liivimaa sõjast Põhjasõjani. Tartu: Tartu Ülikooli Ajaloo ja Arheoloogia Instituut, 2013

Põhjalik koguteos Rootsi võidust / Kaarel Vanamölder

Index Scriptorium Estoniae

Vanamölder, Kaarel, 1981-

2015-01-01

Arvustus: Enn Küng, Margus Laidre, Ivar Leimus, Aivar Põldvee, Anti Selart, Marten Seppel, Kai Tafenau, Ülle Tarkiainen, Enn Tarvel. Eesti ajalugu III: Vene-Liivimaa sõjast Põhjasõjani. Tartu : Tartu Ülikooli Ajaloo ja arheoloogia instituut, 2013

Kehalise väärkohtlemise ja avaliku korra raske rikkumise eristamine : magistritöö / Reelika Ratassepp ; juhendaja Jaan Ginter ; Tartu Ülikool, sotsiaalteaduste valdkond, õigusteaduskond, karistusõiguse osakond

Index Scriptorium Estoniae

Ratassepp, Reelika, 1992-

2018-01-01

Kehalise väärkohtlemise koosseisust enne karistusõiguse revisjoni 1. jaanuaril 2015. Avaliku korra mõistest, avalikus kohas käitumise üldnõudetest ning avaliku korra raskest rikkumisest enne korrakaitseseaduse jõustumis ja pärast jõustumist

Detection of exogenous gene doping of IGF-I by a real-time quantitative PCR assay.

Science.gov (United States)

Zhang, Jin-Ju; Xu, Jing-Feng; Shen, Yong-Wei; Ma, Shi-Jiao; Zhang, Ting-Ting; Meng, Qing-Lin; Lan, Wen-Jun; Zhang, Chun; Liu, Xiao-Mei

2017-07-01

Gene doping can be easily concealed since its product is similar to endogenous protein, making its effective detection very challenging. In this study, we selected insulin-like growth factor I (IGF-I) exogenous gene for gene doping detection. First, the synthetic IGF-I gene was subcloned to recombinant adeno-associated virus (rAAV) plasmid to produce recombinant rAAV2/IGF-I-GFP vectors. Second, in an animal model, rAAV2/IGF-I-GFP vectors were injected into the thigh muscle tissue of mice, and then muscle and blood specimens were sampled at different time points for total DNA isolation. Finally, real-time quantitative PCR was employed to detect the exogenous gene doping of IGF-I. In view of the characteristics of endogenous IGF-I gene sequences, a TaqMan probe was designed at the junction of exons 2 and 3 of IGF-I gene to distinguish it from the exogenous IGF-I gene. In addition, an internal reference control plasmid and its probe were used in PCR to rule out false-positive results through comparison of their threshold cycle (Ct) values. Thus, an accurate exogenous IGF-I gene detection approach was developed in this study. © 2016 International Union of Biochemistry and Molecular Biology, Inc.

Efficient photoreceptor-targeted gene expression in vivo by recombinant adeno-associated virus.

Science.gov (United States)

Flannery, J G; Zolotukhin, S; Vaquero, M I; LaVail, M M; Muzyczka, N; Hauswirth, W W

1997-06-24

We describe a general approach for achieving efficient and cell type-specific expression of exogenous genes in photoreceptor cells of the mammalian retina. Recombinant adeno-associated virus (rAAV) vectors were used to transfer the bacterial lacZ gene or a synthetic green fluorescent protein gene (gfp) to mouse or rat retinas after injection into the subretinal space. Using a proximal murine rod opsin promoter (+86 to -385) to drive expression, reporter gene product was found exclusively in photoreceptors, not in any other retinal cell type or in the adjacent retinal pigment epithelium. GFP-expressing photoreceptors typically encompassed 10-20% of the total retinal area after a single 2-microl injection. Photoreceptors were transduced with nearly 100% efficiency in the region directly surrounding the injection site. We estimate approximately 2.5 million photoreceptors were transduced as a result of the single subretinal inoculation. This level of gene transfer and expression suggests the feasibility of genetic therapy for retinal disease. The gfp-containing rAAV stock was substantially free of both adenovirus and wild-type AAV, as judged by plaque assay and infectious center assay, respectively. Thus, highly purified, helper virus-free rAAV vectors can achieve high-frequency tissue-specific transduction of terminally differentiated, postmitotic photoreceptor cells.

Evaluation of immunologic effect of Enniatin A and quantitative determination in feces, urine and serum on treated Wistar rats.

Science.gov (United States)

Juan, Cristina; Manyes, Lara; Font, Guillermina; Juan-García, Ana

2014-09-01

Study of dietary supplementation with ENN A mycotoxin during 28 days of exposure time on Wistar rats to determinate its levels in serum, urine and feces and, to evaluate the immunologic effect in peripheral blood lymphocytes (PBL) is presented. The first method for ENN A extraction, determination and detection by LC-MS/MS in serum, urine and feces samples is reported. ENN A food dose administrated was detected in serum samples and influenced lymphocyte phenotyping. Levels in serum were founded from the second week of the experiment; reaching values of 4.76 μg/ml on the fourth week, which corresponds to 3.24 μg/ml in blood. PBL as T helper (CD4(+)) were presented in greater percentages compared to control (p ≤ 0.001), while T cytotoxic (CD8(+)) decreased significantly compared to control (p ≤ 0.001). ENN A treatment significantly increased CD4(+)/CD3(+) and CD4(+)/CD8(+) ratios but significantly decreased CD8(+)/CD3(+) ratio. CD4(+)/CD8(+) ratio was 2.94:1, indicating that PBL surface antigen expression and immune status in Wistar rats treated were impaired by the ENN A mycotoxin. Copyright © 2014 Elsevier Ltd. All rights reserved.

The Clerical Establishment in Iran

Science.gov (United States)

1989-06-01

Political Action and Legitimate Domi- nation in Shi’ite Iran: Fourteenth to Eighteenth Centuries, A.D.," Archives Europ~ennes de Sociologie , Vol. 20, No...139 Archives Europ6ennes de Sociologie Bours, (moderate centrist) weekly in Persian, Tehran Conflict Studies, quarterly in English, London Enghelab

Nõutakse Eesti-Läti-Leedu lastefilme / Jaan Ruus

Index Scriptorium Estoniae

Ruus, Jaan, 1938-2017

2005-01-01

Berliini festivali filmiturul tutvustas Baltic Films kõige edukamalt lastefilme nagu Rene Vilbre "Röövlirahnu Martin". Läti animafilmist räägib festivali lastefilmide konkursil plastiliinanimatsiooniga "Kuule, jänes... papa sõidab Londonisse" esinenud Nils Skapans (s 1969). Lisatud tabelid "Balti riigid ja film 2004" ja "Mida Baltikumis mullu kõige enam vaadati?"

Mida sportijad vajavad? / Evelin Kivilo-Paas

Index Scriptorium Estoniae

Kivilo-Paas, Evelin, 1979-

2015-01-01

Sportijate toitainete vajaduse kohta jagavad selgitusi Tallinna Ülikooli terviseteaduste ja spordi instituudi direktor ja spordibioloog Kristjan Port, Synlab Eesti laboriarst dr Anneli Raave-Sepp ja Spordimeditsiini SA spordiarst dr Krista Veevo

В Хельсинки открылась выставка эстонской живописи

Index Scriptorium Estoniae

2010-01-01

Helsingi kunstihoones (Helsingin Taidehalli) avati 15. oktoobril Eesti maalikunsti ülevaatenäitus "Viron värit - 150 vuotta virolaista maalaustaideta Enn Kunilan kokoelamasta" ("Eesti värvid - 150 aastat eesti maalikunsti Enn Kunila kogust"). Kuraator Harry Liivrand, kujundaja Olev Subbi. Helsingis on "Viron värit" avatud 24. novembrini

Feminist Therapy: Not for or by White Women Only.

Science.gov (United States)

Espin, Olivia M.

1993-01-01

Responds to earlier article by Carolyn Zerbe Enns on feminist counseling and psychotherapy, noting that Enns could have elaborated on what the challenge of multiculturalism meant for the future of feminist therapy and counseling. Asserts that tendency to overgeneralize from data and information gathered from experience of white women continues for…

Feminist Counseling/Therapy for the 21st Century.

Science.gov (United States)

Worell, Judith; Robinson, Damon

1993-01-01

Responds to earlier article by Carolyn Zerbe Enns on feminist counseling and psychotherapy. Acknowledges productive impact of Enns's review, but raises some concerns related to the conception of history and some of the conclusions that are reached. Discusses two important parameters that merit more attention, focusing on the future of feminist…

USA-Balti harta 10. aastapäev / Toomas Hendrik Ilves, Valdis Zatlers, Valdas Adamkus

Index Scriptorium Estoniae

Ilves, Toomas Hendrik, 1953-

2008-01-01

Ingl. k. ilmunud: Eesti Elu 18. jaan. 2008, lk. 11, pealk.: Joint statement of the Presidents of the Baltic States; Vaba Eesti Sõna 24. jaan. 2008, lk. 12 ja 27. märts 2008, lk. 12, pealk.: Joint statement 10th anniversary of the U.S.-Baltic Charter. 1998. aasta 16. jaanuaril Valges Majas toimunud tseremoonial allkirjastasid Ameerika Ühendriikide, Leedu, Läti ja Eesti presidendid USA-Balti partnerlusharta. Eesti president Toomas Hendrik Ilves, Läti president Valdis Zatlers ja Leedu president Valdas Adamkus meenutavad oma avalduses seda dokumenti ajaloolise poliitilise teetähisena riikide suhetes

Ilushow enne teatrit

Index Scriptorium Estoniae

2009-01-01

29. mail juhatab Rakvere Teater pangamajas toimuva moe- ja ilushowga sisse oma esimese suvelavastuse - Jean Genet' "Toatüdrukud", lavastaja Hendrik Toompere, kunstnik Ervin Õunapuu. Esietendus 5. juunil pangamajas

Recombinant AAV-mediated BEST1 transfer to the retinal pigment epithelium: analysis of serotype-dependent retinal effects.

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Karina E Guziewicz

Full Text Available Mutations in the BEST1 gene constitute an underlying cause of juvenile macular dystrophies, a group of retinal disorders commonly referred to as bestrophinopathies and usually diagnosed in early childhood or adolescence. The disease primarily affects macular and paramacular regions of the eye leading to major declines in central vision later in life. Currently, there is no cure or surgical management for BEST1-associated disorders. The recently characterized human disease counterpart, canine multifocal retinopathy (cmr, recapitulates a full spectrum of clinical and molecular features observed in human bestrophinopathies and offers a valuable model system for development and testing of therapeutic strategies. In this study, the specificity, efficiency and safety of rAAV-mediated transgene expression driven by the human VMD2 promoter were assessed in wild-type canine retinae. While the subretinal delivery of rAAV2/1 vector serotype was associated with cone damage in the retina when BEST1 and GFP were co-expressed, the rAAV2/2 vector serotype carrying either GFP reporter or BEST1 transgene under control of human VMD2 promoter was safe, and enabled specific transduction of the RPE cell monolayer that was stable for up to 6 months post injection. These encouraging studies with the rAAV2/2 vector lay the groundwork for development of gene augmentation therapy for human bestrophinopathies.

Preparation and characterization of inorganic and organic coatings on AZ91D magnesium alloy with electroless plating pretreatment

Energy Technology Data Exchange (ETDEWEB)

Zhang, S.Y.; Li, Q.; Zhang, H.X.; Wang, S.Y.; Liu, F. [School of Chemistry and Chemical Engineering, Southwest University Chongqing, 400715 (China); Yang, X.K. [School of Materials Science and Engineering, Southwest University Chongqing, 400715 (China)

2011-09-15

In this paper, a protective coating scheme was applied for the corrosion protection of AZ91D magnesium alloy. Electroless Ni coating (EN coating) as bottom layer, electrodeposited Ni coating (ENN coating), and silane-based coating (ENS coating) as top layer, respectively, were successfully prepared on AZ91D magnesium alloy by combination techniques. Scanning electron microscopy and X-ray diffraction were employed to investigate the surface and phase structure of coatings, respectively. The electrochemical corrosion behaviors of coatings in neutral 3.5 wt% NaCl solution were evaluated by electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization techniques. The corrosion testing showed that the three kinds of coatings all could provide corrosion protection for AZ91D magnesium alloy to a certain extent, and the corrosion resistance of ENN and ENS was superior to EN. In order to further study the corrosion protection properties of ENN and ENS, a comparative investigation on the evolution of EIS of ENN and ENS was carried out by dint of immersion test in neutral 3.5 wt% NaCl solution. The results indicated that, compared with ENN, the ENS could provide longer corrosion protection for AZ91D magnesium alloy. It is significant to determine the barrier effect of each coating, which could provide reference for industry applications. (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

Eesti kunstiaarded rändasid üle mere: "Ma poleks õiglane, kui hoiaksin maalid vaid endale" / Oliver Õunmaa

Index Scriptorium Estoniae

Õunmaa, Oliver

2010-01-01

Eesti maalikunsti ülevaatenäitus "Eesti värvid - 150 aastat eesti maalikunsti Enn Kunila kogust" Helsingi Taidehallis 24. novembrini 2010. Enn Kunila, näituse kuraatori Harry Liivranna, Helsingi kunstihoone juhataja kohusetäitja Taru Tappola kommentaarid. Näituse kujundas Olev Subbi, soomekeelse raamatu näituse kohta koostas Eero Epner

Striatal pre-enkephalin overexpression improves Huntington's disease symptoms in the R6/2 mouse model of Huntington's disease.

Directory of Open Access Journals (Sweden)

Stéphanie Bissonnette

Full Text Available The reduction of pre-enkephalin (pENK mRNA expression might be an early sign of striatal neuronal dysfunction in Huntington's disease (HD, due to mutated huntingtin protein. Indeed, striatopallidal (pENK-containing neurodegeneration occurs at earlier stage of the disease, compare to the loss of striatonigral neurons. However, no data are available about the functional role of striatal pENK in HD. According to the neuroprotective properties of opioids that have been recognized recently, the objective of this study was to investigate whether striatal overexpression of pENK at early stage of HD can improve motor dysfunction, and/or reduce striatal neuronal loss in the R6/2 transgenic mouse model of HD. To achieve this goal recombinant adeno-associated-virus (rAAV2-containing green fluorescence protein (GFP-pENK was injected bilaterally in the striatum of R6/2 mice at 5 weeks old to overexpress opioid peptide pENK. Striatal injection of rAAV2-GFP was used as a control. Different behavioral tests were carried out before and/or after striatal injections of rAAV2. The animals were euthanized at 10 weeks old. Our results demonstrate that striatal overexpression of pENK had beneficial effects on behavioral symptoms of HD in R6/2 by: delaying the onset of decline in muscular force; reduction of clasping; improvement of fast motor activity, short-term memory and recognition; as well as normalization of anxiety-like behavior. The improvement of behavioral dysfunction in R6/2 mice having received rAAV2-GFP-pENK associated with upregulation of striatal pENK mRNA; the increased level of enkephalin peptide in the striatum, globus pallidus and substantia nigra; as well as the slight increase in the number of striatal neurons compared with other groups of R6/2. Accordingly, we suggest that at early stage of HD upregulation of striatal enkephalin might play a key role at attenuating illness symptoms.

Delivery and evaluation of recombinant adeno-associated viral vectors in the equine distal extremity for the treatment of laminitis.

Science.gov (United States)

Mason, J B; Gurda, B L; Van Wettere, A; Engiles, J B; Wilson, J M; Richardson, D W

2017-01-01

Our long-term aim is to develop a gene therapy approach for the prevention of laminitis in the contralateral foot of horses with major musculoskeletal injuries and non-weightbearing lameness. The goal of this study was to develop a practical method to efficiently deliver therapeutic proteins deep within the equine foot. Randomised in vivo experiment. We used recombinant adeno-associated viral vectors (rAAVs) to deliver marker genes using regional limb perfusion through the palmar digital artery of the horse. Vector serotypes rAAV2/1, 2/8 and 2/9 all successfully transduced equine foot tissues and displayed similar levels and patterns of transduction. The regional distribution of transduction within the foot decreased with decreasing vector dose. The highest transduction values were seen in the sole and coronary regions and the lowest transduction values were detected in the dorsal hoof-wall region. The use of a surfactant-enriched vector diluent increased regional distribution of the vector and improved the transduction in the hoof-wall region. The hoof-wall region of the foot, which exhibited the lowest levels of transduction using saline as the vector diluent, displayed a dramatic increase in transduction when surfactant was included in the vector diluent (9- to 81-fold increase). In transduced tissues, no significant difference was observed between promoters (chicken β-actin vs. cytomegalovirus) for gene expression. All horses tested for vector-neutralising antibodies were positive for serotype-specific neutralising antibodies to rAAV2/5. The current experiments demonstrate that transgenes can be successfully delivered to the equine distal extremity using rAAV vectors and that serotypes 2/8, 2/9 and 2/1 can successfully transduce tissues of the equine foot. When the vector was diluted with surfactant-containing saline, the level of transduction increased dramatically. The increased level of transduction due to the addition of surfactant also improved the

L’Union européenne: un champion en trompe l’œil de l’aide publique au développement

Directory of Open Access Journals (Sweden)

Yann Richard

2008-06-01

Full Text Available L’Union européenne est le premier bailleur de fonds de l’aide publique au développement dans le monde. Elle y consacre des sommes plus importantes que les États-Unis et le Japon et elle domine dans l’aide au développement perçue par la quasi-totalité des pays bénéficiaires (pays en développement et pays en transition. Elle est le premier donateur aussi bien pour l’importance de sommes dépensées que pour l’effort consenti (l’aide rapportée au revenu national brut ou bien au nombre des habitants des pays donateurs. Mais ce statut de premier donateur est en partie illusoire. Cette domination vient de l’addition artificielle de l’aide versée par la Commission européenne et de l’aide versée par les pays membres de l’UE 15. Or, l’analyse des statistiques de l’aide au développement montre que ces deux types de donateurs ne suivent pas les mêmes logiques pour l’allocation géographique de leur aide. L’aide au développement versée par l’Union, comparativement à celle versée par le Japon et les États-Unis, semble avoir un caractère universel. Cette apparente générosité ne cache-t-elle pas une faiblesse ? On peut se demander si l’aide européenne ne souffre pas en réalité d’une trop grande dispersion. Par ailleurs, la géographie de l’aide versée par l’UE ne semble pas correspondre au discours européen qui met au sommet de l’agenda politique de l’Union le développement des relations avec ses voisins géographiques. Ce manque de cohérence transparaît dans l’évolution inverse des aides versées par la Commission (en augmentation et par les pays membres de l’UE (en baisse. L’Union s’est bel et bien engagée dans une réforme de la gouvernance de son aide au développement (déclaration de Paris, Objectifs du Millénaire pour le développement. Néanmoins, les tendances que nous soulignons dans cet article montrent que les résultats sont encore à venir. La faible lisibilit

ENSEIGNER LES DISPARITÉS SOCIO-SPATIALES AVEC HYPERATLAS: LE CAS DE L’UNION EUROPÉENNE

Directory of Open Access Journals (Sweden)

María Pigaki

2014-12-01

-spatiales qui existent au niveau régional dans l’Union européenne. HyperAtlas est non seulement un outil pour développer la pensée spatiale par l’analyse spatiale. Ce est aussi une ressource éducative à travers laquelle les élèves apprennent à exercer la citoyenneté, à comprendre les problèmes sociaux de caractère territorial et à proposer des solutions. Le texte décrit les objectifs, la méthodologie et les conditions d’expérimentation de HyperAtlas dans la classe de géographie à deux lycées différentes, l’un en Grèce, l’autre en France. Les similitudes et les différences entre ces deux expérimentations, mais les conclusions sur l’utilisation de HyperAtlas montrent comment HyperAtlas est un outil pour la recherche de l’espace à l’école. Mots-clés: HyperAtlas, analyse spatiale, disparités territoriales, éducation pour la citoyenneté.   

An integrated sample preparation to determine coccidiostats and emerging Fusarium-mycotoxins in various poultry tissues with LC-MS/MS.

Science.gov (United States)

Jestoi, Marika; Rokka, Mervi; Peltonen, Kimmo

2007-05-01

The usefulness of an existing sample preparation technique used for ionophoric coccidiostats (lasalocid, monensin, salinomycin and narasin) was applied in the analysis of emerging Fusarium-mycotoxins beauvericin (BEA) and enniatins (ENNs) in poultry tissues (liver and meat). Also, maduramicin and liver as a new sample matrix was introduced. The developed methods were validated and applied for the determination of coccidiostats and BEA/ENNs in Finnish poultry tissues in 2004-2005. The validation parameters demonstrated that the integrated sample preparation technique is applicable to the parallel determination of these contaminants in poultry tissues. Of the samples analysed (276 meat and 43 liver), only trace levels of LAS, MON, SAL, NAR and MAD were detected in 7, 3, 5, 6 and 4% of the samples, respectively. Interestingly, for the first time, traces of BEA and ENNs could also be detected in animal tissues. BEA and ENNs A, A1, B and B1 were found in 2, 0.3, 0.6, 4 and 3% of the samples, respectively. The simultaneous presence of coccidiostats and mycotoxins was detected in three turkey samples in 2004.

Nouvelles données sur la paléobiogéographie des genres Septimaniceras Fauré, 2002 et Crassiceras Merla, 1932 (Ammonitina) du Toarcien moyen

OpenAIRE

Jattiot, Romain; Rulleau, Louis; Latutrie, Benjamin

2017-01-01

Depuis sa première description, le genre Septimaniceras Fauré, 2002 a été systématiquement considéré comme un genre endémique, avec une répartition géographique limitée à la région des Causses et du Languedoc (France, province nord-ouest européenne). De même, l’espèce Crassiceras bayani (Dumortier, 1874), d’affinités méditerranéennes, était interprétée comme une forme spécifiquement nord-ouest européenne cantonnée au sud-est de la France, et ne dépassant pas la région lyonnaise en latitude no...

Helsingis avatakse suur Eesti maalinäitus

Index Scriptorium Estoniae

2010-01-01

Eesti maalikunsti ülevaatenäitus "Viron värit - 150 vuotta virolaista maalaustaideta Enn Kunilan kokoelamasta" ("Eesti värvid - 150 aastat eesti maalikunsti Enn Kunila kogust") Helsingi kunstihoones (Helsingin Taidehalli) 16. oktoobrist 24. novembrini 2010. Näituse kuraator Harry Liivrand, kujundaja Olev Subbi. Väljapanekut saatva raamatu on koostanud Eero Epner, kujundanud Tiit Jürna

Europe is the new home market for Sonex / Eimundas Maciulis

Index Scriptorium Estoniae

Maciulis, Eimundas

2004-01-01

Leedu suurima arvutitootja Sonex turundusjuht ettevõtte arengust, brändidest, konkurentsieelistest võrreldes teiste arvutitootjatega, turundustegevusest ja välisturgudega seotud tulevikuplaanidest

Soojalt sojast / Ülle Hõbemägi

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Hõbemägi, Ülle

2008-01-01

Sojast, sojatoodetest ning sojatoitudest räägivad aroomterapeut ja massöör Marge Teder, Soya Foods Baltic OÜ müügidirektor Meeli Matsalu, tudeng ja taimetoitlane Loore-Emilie Raav ning Angelica Udeküll

Leishmaniose canine dans le Rif (côte méditerranéenne marocaine : enquête séro-épidémiologique

Directory of Open Access Journals (Sweden)

Rami M.

2003-03-01

Full Text Available Dans le but d'évaluer la situation de la leishmaniose canine dans le foyer situé dans le versant Nord-Est de la chaîne montagneuse du Rif, une enquête séro-épidémiologique a été menée dans différentes localités de la province de Nador (côte méditerranéenne marocaine. Des échantillons de sérum collectés sur 257 chiens ont été analysés par les techniques d'immunofluorescence indirecte (IFI et d'enzyme-linked immunosorbent assay (ELISA pour détecter les anticorps anti-Leishmania infantum. Les résultats montrent que 48 (18,7 % des chiens examinés sont positifs en IFI et 54 (21,0 % le sont en ELISA ; la concordance des deux méthodes étant de 96,1 %. D'autre part, le taux d'infection est significativemenl plus élevé chez les chiens âgés de plus de quatre ans et ne dépend pas du sexe de l'animal. Les symptômes les plus fréquemment relevés sur les chiens séropositifs sont la polyadénite (57,4 %, l'amyotrophie (51,9 % et les lésions cutanéo-muqueuses ( 25,9 %. Cependant, 38,9 % de ces mêmes chiens ne présentent pas de symptômes pouvant évoquer la leishmaniose. Des Leishmania isolées sur trois des chiens examinés ont toutes été identifiées comme étant L. infantum MON-1. Ces résultats montrent que le versant Nord-Est des montagnes du Rif est l'un des foyers les plus actifs de leishmaniose viscérale dans le pourtour méditerranéen et confirment le rôle que joue le chien comme principal réservoir de L. infantum.

Les débouchés énergétiques de l'agriculture de la Communauté Economique Européenne Energy Outlets for Agriculture in the European Economic Community

Directory of Open Access Journals (Sweden)

Becker J. J.

2006-11-01

Full Text Available L'agriculture de la Communauté Economique Européenne (CEE connaît une crise profonde au niveau de ses débouchés. Ainsi, même dans un contexte où l'indépendance énergétique n'est plus une préoccupation majeure, une production massive d'énergie par l'agriculture reste un projet à priori digne d'intérêt. Cette étude précise les modalités d'application concrètes d'une telle politique et tente une évaluation économique permettant d'apprécier la viabilité d'un tel schéma. Elle présente, en particulier, un positionnement par rapport aux solutions agricoles plus classiques actuellement mises en oeuvre et qui pourtant ne sont guère satisfaisantes. Agriculture in the EEC is in the midst of a deep crisis concerning its outlets. Even in a context where energy independence is no longer a major preoccupation, massive energy production by agriculture is still a problem worthy of interest, a priori. This article specifies concrete procedures for applying such a policy and tries to make an economic evaluation to assess the viability of such a scheme. In particular, it describes a position in relation to the most conventional agricultural solutions now being implemented and that are proving not to be very satisfactory.

Memento ettepanek president Ilvesele ja välisminister Paetile

Index Scriptorium Estoniae

2010-01-01

Eesti Memento Liidu juhatuse esimehe Enn Tarto kirjast president Toomas Hendrik Ilvesele ja välisminister Urmas Paetile seoses nende sõiduga Moskvasse Suure Isamaasõja võidu aastapäeva tähistamise pidustustele. Ilmunud ka: Vaba Eesti Sõna 6. mai 2010, lk. 3, pealk.: Enn Tarto annab Moskvasse sõitvale presidendile nõu. Töövisiit Moskvasse 8.-9.05.2010

A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair

Directory of Open Access Journals (Sweden)

Leclerc Xavier

2009-04-01

Full Text Available Abstract Background Current strategies for gene therapy of inherited diseases consist in adding functional copies of the gene that is defective. An attractive alternative to these approaches would be to correct the endogenous mutated gene in the affected individual. This study presents a quantitative comparison of the repair efficiency using different forms of donor nucleic acids, including synthetic DNA oligonucleotides, double stranded DNA fragments with sizes ranging from 200 to 2200 bp and sequences carried by a recombinant adeno-associated virus (rAAV-1. Evaluation of each gene repair strategy was carried out using two different reporter systems, a mutated eGFP gene or a dual construct with a functional eGFP and an inactive luciferase gene, in several different cell systems. Gene targeting events were scored either following transient co-transfection of reporter plasmids and donor DNAs, or in a system where a reporter construct was stably integrated into the chromosome. Results In both episomal and chromosomal assays, DNA fragments were more efficient at gene repair than oligonucleotides or rAAV-1. Furthermore, the gene targeting frequency could be significantly increased by using DNA repair stimulating drugs such as doxorubicin and phleomycin. Conclusion Our results show that it is possible to obtain repair frequencies of 1% of the transfected cell population under optimized transfection protocols when cells were pretreated with phleomycin using rAAV-1 and dsDNA fragments.

Grybauskaite's two years in office / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Leedu presidenti Dalia Grybauskaitet iseloomustab suur populaarsus rahva seas ning tagasihoilik suhtlemine poliitika-, äri- ja diplomaatiaringkondadega. Grybauskaite seisukohad feminismi- ja sooküsimustes

Speaker on verge of resignation

Index Scriptorium Estoniae

2008-01-01

Leedu parlamendi spiikri Viktoras Muntianase umbusaldusavaldusele kirjutasid alla nii opositsiooni- kui ka koalitsiooniparteide poliitikud. Muntianas on seotud altkäemaksu skandaaliga Kaunase kruntide erastamisel

Recognition and enforcement of foreign judgements in the Republic of Lithuania = Признание и приведение к исполнению решений иностранных судов в Литовской Республике / Tatjana

Index Scriptorium Estoniae

Žukauskienė, Tatjana

2010-01-01

Tunnustamisprotseduuridest (eksekvaaturi väljaandmine ; registreerimine), neljast mudelist välisriigi kohtulahendite tunnustamisel ja täitmiseks lubamisel, Leedu tsiviilkohtumenetluse seadustikust ja rahvusvahelistest lepingutest

Parliamentary speaker on thin ice / Laima Vaiga

Index Scriptorium Estoniae

Vaiga, Laima

2008-01-01

Leedu seimi spiikri Viktoras Muntianase koht on ohus, kuna tema juhitav Kodanikudemokraatia Partei võib koalitsioonist lahkuda. Parlamendist lahkus Kodanikudemokraatia Partei saadik Algimantas Matulevicius

Iglesiase-Anett sai eluajal Kolga-Jaani ausamba / Rainer Kerge

Index Scriptorium Estoniae

Kerge, Rainer, 1977-

2008-01-01

ETV "Erisaate" eestvedamisel püstitati Kolga-Jaani ausammas tühisusele. Ausammas kujutab Anett Arnimit, kes oli esikohal Eesti Ekspressi koostatud tühiste kuulsuste edetabelis. Kuju valmistasid skulptuuritudengid Loore-Emilie Raav, Regina Kuningas ja Kadri Allekand

Serum levels of organochlorine pesticides in the French adult population: the French National Nutrition and Health Study (ENNS), 2006-2007.

Science.gov (United States)

Saoudi, Abdessattar; Fréry, Nadine; Zeghnoun, Abdelkrim; Bidondo, Marie-Laure; Deschamps, Valérie; Göen, Thomas; Garnier, Robert; Guldner, Laurence

2014-02-15

Although most organochlorine (OC) pesticides were banned in France in the 1970s and 1980s, they remain a source of public concern. Because of their high persistence in the environment, they are still detected in foodstuffs, leading to continued human exposure. The purpose of this study was to assess the distribution of serum organochlorine (OC) pesticides in the French adult population and to identify the main risk factors for p,p'-dichlorodiphenyl dichlorethylene (DDE), β-hexachlorocyclohexane (β-HCH) and hexachlorobenzene (HCB). The selected OC pesticides (HCB, DDE, DDT, α-HCH, β-HCB and γ-HCH) were measured in serum samples collected in 2006-2007 from 386 persons (aged 18-74 years) randomly selected among the participants in the clinical and biological component of the French Nutrition and Health Survey (Etude Nationale Nutrition Santé [ENNS]), a cross-sectional survey carried out in the general population. Collected data included biological samples, socio-demographic characteristics, and data about environmental and occupational exposure factors. Of the six OC pesticides investigated, the highest concentrations were observed for HCB, β-HCH and DDE. Median serum concentrations were as follows: 22.8 ng/g lipid for HCB, 0.74 and 27.0 ng/g lipid, respectively, for α- and ß-HCH, and 3.8 and 104.6 ng/g lipid, respectively, for DDT and DDE. Lindane (γ-HCH) was detected in approximately 10% of the sample. OC pesticide levels in serum in French adults were higher (except for DDT and DDE) than those observed in American, Canadian and German populations and generally lower than or in the same range as those observed in other European countries. The low serum DDT/DDE ratio in the present study (3.7%) would suggest that the concentrations observed for these two OC pesticides were mainly the result of past exposure. The most important predictors of serum DDE, HCB and β-HCH concentrations among the French adult population included individual factors (age, gender

Valinskas attacks Grybauskaite / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Arunas Valinskas leidis, et ta on sobiv kandidaat Leedu kultuuriministri kohale. Arunas Valinskase ja president Dalia Grybauskaite vahelisest konfliktist. Arunas Valinskase kriitika Dalia Grybauskaite aadressil

Epohha golubogo miloserdija / Mihhail Tuzhikov

Index Scriptorium Estoniae

Tuzhikov, Mihhail

2004-01-01

Gaasi tarbimise suurenemine ning ressursid maailmas. Läti, Eesti ja Leedu gaasiturg. Diagrammid: Maailma gaasivarud 01 01.2004, %; Maagaasi tarbimine Baltimaades ; Maagaasi tarbimise prognoos Baltimaades

Embattled MPs lose immunity / Justinas Vainilavicius

Index Scriptorium Estoniae

Vainilavicius, Justinas

2008-01-01

Leedu Seim otsustas parlamendiliikmetelt Rokas Zilinskaselt, Viktor Uspaskihhilt ja Vytautas Gapsyselt ära võtta saadikupuutumatus. Seega jätkuvad parlamendisaadikute üle pooleliolevad kohtuprotsessid

Adamkus suggests that both Uspaskich, Zuokas resign posts / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

President Valdas Adamkus soovitas majandusminister Viktor Uspaskihhil ja Vilniuse linnapeal Arturas Zuokasel tagasi astuda, kuna nendevaheline konflikt kahjustab Leedu mainet ja julgustas poliitikutega seotud uurimist

The results of regulation policy changes on food grain market in Lithuania on the way to the European Union / Valdemaras Makutenas, Daiva Makuteniene

Index Scriptorium Estoniae

Makutenas, Valdemaras

2003-01-01

Leedu ja Euroopa Liidu teraviljaturu reguleerimise võrdlus. EL teravilja- ja teraviljasaaduste poliitika ülevõtmine muutumatul kujul oleks kasulik teravilja- ja õlikultuuride kasvatajatele. Tabelid

Adamkus tries hand at mediating in eye of Ukrainian maelstrom

Index Scriptorium Estoniae

2004-01-01

Leedu president Valdas Adamkus sõitis Leonid Kutshma kutsel Ukrainasse vahendamaks läbirääkimisi presidendikandidaatide - opositsiooniliider Viktor Jushtshenko ja peaminister Viktor Janukovitshi vahel

State security agents detain journalist, spark major political backlash

Index Scriptorium Estoniae

2006-01-01

Leedu julgeolekuameti peadirektor Avydas Pocius lasi läbi otsida ajalehe Laisvas Laikrastis toimetuse, süüdistades ajalehte riigisaladuse kuritarvitamises. Ajakirjanikud nimetavad seda ajakirjandusvabaduse rikkumiseks

Insurance bankruptcy creates political storm

Index Scriptorium Estoniae

2005-01-01

Hoolimata peaminister Algirdas Brazauskase nõudmisest, keeldus Leedu kindlustusinspektsiooni juht Edvinas Vasilis-Vasiliauskas oma kohalt tagasi astumast, kuna soovib viia lõpule kindlustusseltsi Ingo Baltic pankrotiprotsessi

Civis Europeus Sum oder: Chamberlain spielen / Robert von Lucius

Index Scriptorium Estoniae

Lucius, Robert von

2014-01-01

Baltimaade presidentidele Kuldse Victoria auhinna omistamisest. Eesti ja Leedu presidentide kõnedest. Toomas Hendrik Ilvese vestlusest Joachim Gauck'iga ja mõtteavaldustest Saksa Välissuhete Nõukogus

Opposition failed to impeach energy minister / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

10. märtsil ei saanud Leedu opositsioon energeetikaminister Arvydas Sekmokasele umbusaldust avaldada. Predisent Dalia Grybauskaite toetab samuti Arvydas Sekmokase jätkamist ministrina. Valitsuse energeetikaplaanid

Citigroup boosts investment in retail trade

Index Scriptorium Estoniae

2005-01-01

Investeerimisgrupi Citigroup tütarfirma investeerib Leedu jaekaubandusketti Iki kokku 50 miljonit eurot. Selle tulemusena omandab Citigroup firma Palink, mis omakorda on Iki kaupluste omanik, aktsiaid

Emploi et croissance | Page 17 | CRDI - Centre de recherches pour ...

International Development Research Centre (IDRC) Digital Library (Canada)

Le documentaire Forward Home, qui a été produit dans le cadre du projetAvantages découlant de la circulation des cerveaux des pays de la CARICOM ainsi que du tourisme et des investissements de la part de la diaspora caribéenne du CRDI, révèle la puissance économique de la diaspora caribéenne. Read more about ...

Première de Forward Home, un documentaire sur la puissance ...

International Development Research Centre (IDRC) Digital Library (Canada)

26 avr. 2016 ... Le documentaire Forward Home, qui a été produit dans le cadre du projetAvantages découlant de la circulation des cerveaux des pays de la CARICOM ainsi que du tourisme et des investissements de la part de la diaspora caribéenne du CRDI, révèle la puissance économique de la diaspora caribéenne.

"Дева Маали" пожизненно обручена с Вейко Ыунпуу / Борис Тух

Index Scriptorium Estoniae

Тух, Борис, 1946-

2010-01-01

Eesti Filmiajakirjanike Ühingu poolt kinos "Sõprus" väljaantud auhindadest: parimaks filmiks tunnistati ja anti üle Neitsi Maali auhind ning rahaline preemia Veiko Õunpuu mängufilmile "Püha Tõnu kiusamine". Parima filmiajakirjaniku preemia sai Enn Säde ja eripreemia Ilmar Raag blogi "Ilmari jäljed liival enne vihmasadu" eest. Parimaks välisfilmiks valiti Laurent Cantet' film "Seinte vahel" (Prantsusmaa 2008)

Teledokumentalistika hiilgusest ja viletsusest / Maria Ulfsak

Index Scriptorium Estoniae

Ulfsak, Maria, 1981-

2004-01-01

Leedu teleprogrammide ja -filmide festivalil "Amber arch" ("Merevaigust arkaad") võitis peapreemia Priit Valkna dokumentaalfilm "Hunt", mille tootis Allfilm ETV "Eesti lugude" dokumentaalsarja. Ka teistest festivali filmidest

Adamkus, Zatlers : new nuclear plant a common Baltic goal

Index Scriptorium Estoniae

2007-01-01

Leedu president Valdas Adamkus kinnitas ajakirjanikele, et nelja riigi koostööl ehitatav tuumaelektrijaam tuleb kindlasti. Läti president Zatlers rõhutas uute tehnoloogiate turvalisuse vajalikkust

The year of political advertising - now! / Marius Vaupsas

Index Scriptorium Estoniae

Vaupsas, Marius

2004-01-01

BBDO Lukrecijos Reklama juht prognoosib 2004. aastat Leedu reklaamiturul, poliitilisest reklaamist, poliitikute ja reklaamispetsialistide vahelistest suhetest ning reklaamiagentuuride rollist poliitilistes valimiskampaaniates. Lisa: Some examples of successful cooperation

Massimiliano Fuksas - pilvede taltsutaja

Index Scriptorium Estoniae

2006-01-01

Leedu juurtega itaalia arhitekti loomingust. Lähemalt Milano uuest messikeskusest, Emporio Armani hoonest Hongkongis, Bassano del Grappa uurimis- ja multimeediakeskusest Itaalias, Rooma Kongressikeskusest La Nuvola, 26 ill

Metal shipment raises frightening questions / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu majandusministeerium lubas Vene ettevõttel eksportida Venemaale berülliumi, mida kasutatakse tuumapommide ja -reaktorite ehitamisel. Berülliumi transpordiga seoses on Leedul varemgi arusaamatusi esinenud

Eesti oma tuumajaam tulgu aastaks 2025 / Erik Müürsepp

Index Scriptorium Estoniae

Müürsepp, Erik

2008-01-01

Majandus- ja kommunikatsiooniministeeriumi koostatud arengukavast, milles pakutakse välja rajada Eestisse väiksem tuumaelektrijaam. Diagrammid: Rohkem tuumaenergiat = odavam elekter. Lisa: Leedu jaam küsitav

Municipal elections: Zuokas-led non-party coalition conquers Vilnius / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

27. veebruaril Leedus peetud kohalikel valimistel kogus enim hääli Leedu Sotsiaaldemokraatlik Partei. Praeguse Vilniuse linnapea Arturas Zuokase koalitsioon on saanud 12 kohta Vilniuse linnavolikogus

TV chief spends election night in hotel utility closet / Justinas Vainilavicius

Index Scriptorium Estoniae

Vainilavicius, Justinas

2008-01-01

Leedu Rahvusliku Raadio ja Televisiooni peadirektor Audrius Siaurusevicius peab tõenäoliselt ametist lahkuma, kuna oli parlamendivalimiste õhtul Sotsiaaldemokraatide peo ülekande ajal alkoholijoobes. Ülevaade juhtunust

MPs place ban on Paksas / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2004-01-01

Leedus jõustus presidendivalimiste seaduse parandus, mis keelab presidendiametist tagandatud isikul viie aasta jooksul riigipeaks pürgimise. Leedu keskvalimiskomisjon otsustas mitte registreerida presidendikandidaadiks endist riigipead Rolandas Paksast

Labour Party's fate hangs in balance / Kimberly Kweder

Index Scriptorium Estoniae

Kweder, Kimberly

2007-01-01

Koduarestis oleval kandidaadil Viktor Uspaskihhil õnnestus oma valimisringkonnas koguda 20 % häältest. Tööpartei kandidaati süüdistatakse pettuses eelmistel valmistel. Leedu politoloogide kommentaarid

Hansapank is the best Baltic brand / Aigars Rungis

Index Scriptorium Estoniae

Rungis, Aigars

2003-01-01

Eesti, Läti ja Leedu ettevõtete turundustegevusest Baltimaades. Kommenteerivad Robin K. Rindehed, Kristiina Kõiv, Margus Sameli ja Kaarel Karu. Vt. samas intervjuud Põlva Piima turundusjuhi Sven Puussepaga

Parliament refuses pensions to apparatchiks / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2002-01-01

Leedu parlament lükkas tagasi seaduseelnõu pensionide tõstmise kohta Teise maailmasõja veteranidele, kes olid Nõukogude okupatsiooni ajal kõrgetel kohtadel kommunistlikus parteis ja julgeolekuteenistuses

The relationships between individual values, collectivistic attitudes, and organisational culture among the Russian-speaking employees in Estonia and Lithuania / Rebekka Vedina, Elina Tolmats, Maaja Vadi...[jt.

Index Scriptorium Estoniae

2004-01-01

Seostest kollektivistlike hoiakute ja organisatsioonikultuuri tajumise vahel vene keelt kõnelevate töötajate hulgas Leedu organisatsioonis. Võrdlus Eestis varem läbi viidud uurimuse tulemustega. Tabelid

Application of ex ante measures in political delegation : the case of Lithuania / Giedrius Zhvaliauskas

Index Scriptorium Estoniae

Zhvaliauskas, Giedrius

2004-01-01

Autor analüüsib poliitilise võimu ja vastutuse delegeerimist Leedu poliitikamaastikul, arutleb poliitiliste parteide rolli üle parlamendivalimistel, otsib põhjuseid parlamendiliikmete parteilisele ebalojaalsusele. Skeemid. Tabelid

Russia puts Europe to a test of morality / Vytautas Landsbergis ; interv. Sven Becker

Index Scriptorium Estoniae

Landsbergis, Vytautas

2006-01-01

Euroopa Parlamendi liige vastab küsimustele, mis puudutavad Leedut Euroopa Liidu liikmesriigina, Euroopa Liidu ja Venemaa suhteid, Vene-Saksa gaasitoru ja Leedu sisepoliitikat. Lisa: Vytautas Landsbergise CV

Konsum CAPM og CAPM anomalier: en empirisk studie av det amerikanske aksjemarkedet

OpenAIRE

Tennebeck, Kristoffer; Torgrimsen, Stian

2015-01-01

Er konsumbeta et bedre mål på systematisk risiko enn markedsbeta? I denne oppgaven sammenligner vi den konsumbaserte kapitalverdimodellen (CCAPM) med kapitalverdimodellen (CAPM) for å vurdere deres evne til å forklare forskjellige systematiske faktorer i aksjemarkedet, ved å sette måleperioden fra et kvartal til tilsvarende kvartal påfølgende år. Vi finner mer støtte for CCAPM enn for den tradisjonelle CAPM. nhhmas

Publications | Page 19 | CRDI - Centre de recherches pour le ...

International Development Research Centre (IDRC) Digital Library (Canada)

Le documentaire Forward Home, qui a été produit dans le cadre du projet Avantages découlant de la circulation des cerveaux des pays de la CARICOM ainsi que du tourisme et des investissements de la part de la diaspora caribéenne du CRDI, révèle la puissance économique de la diaspora caribéenne. De la ferme à la ...

Positron Emission Tomography Imaging Demonstrates Correlation between Behavioral Recovery and Correction of Dopamine Neurotransmission after Gene Therapy

International Nuclear Information System (INIS)

Leriche, L.; Besret, L.; Gregoire, M.C.; Deglon, N.; Hantraye, Ph.; Leriche, L.; Besret, L.; Gregoire, M.C.; Deglon, N.; Hantraye, Ph.; Bjorklund, T.; Breysse, N.; Carlsson, T.; Kirik, D.; Dolle, F.; Mandel, R.J.; Kirik, D.

2009-01-01

In vivo gene transfer using viral vectors is an emerging therapy for neuro-degenerative diseases with a clinical impact recently demonstrated in Parkinson's disease patients. Recombinant adeno-associated viral (rAAV) vectors, in particular, provide an excellent tool for long-term expression of therapeutic genes in the brain. Here we used the [ 11 C]raclopride [(S)-(-)-3, 5-dichloro-N-((1-ethyl-2-pyrrolidinyl)methyl)-2-hydroxy- 6-methoxybenzamide] micro-positron emission tomography (PET) technique to demonstrate that delivery of the tyrosine hydroxylase (TH) and GTP-cyclohydrolase 1 (GCH1) enzymes using an rAAV5 vector normalizes the increased [ 11 C]raclopride binding in hemi-parkinsonian rats. Importantly, we show in vivo by micro-PET imaging and postmortem by classical binding assays performed in the very same animals that the changes in [ 11 C]raclopride after viral vector-based enzyme replacement therapy is attributable to a decrease in the affinity of the tracer binding to the D2 receptors, providing evidence for reconstitution of a functional pool of endogenous dopamine in the striatum. Moreover, the extent of the normalization in this non-invasive imaging measure was highly correlated with the functional recovery in motor behavior. The PET imaging protocol used in this study is fully adaptable to humans and thus can serve as an in vivo imaging technique to follow TH+GCH1 gene therapy in PD patients and provide an additional objective measure to a potential clinical trial using rAAV vectors to deliver L-3, 4-dihydroxyphenylalanine in the brain. (authors)

Generation of insulin-producing human mesenchymal stem cells using recombinant adeno-associated virus.

Science.gov (United States)

Kim, Jeong Hwan; Park, Si-Nae; Suh, Hwal

2007-02-28

The purpose of current experiment is the generation of insulin-producing human mesenchymal stem cells as therapeutic source for the cure of type 1 diabetes. Type 1 diabetes is generally caused by insulin deficiency accompanied by the destruction of islet beta-cells. In various trials for the treatment of type 1 diabetes, cell-based gene therapy using stem cells is considered as one of the most useful candidate for the treatment. In this experiment, human mesenchymal stem cells were transduced with AAV which is containing furin-cleavable human preproinsulin gene to generate insulin-producing cells as surrogate beta-cells for the type 1 diabetes therapy. In the rAAV production procedure, rAAV was generated by transfection of AD293 cells. Human mesenchymal stems cells were transduced using rAAV with a various multiplicity of infection. Transduction of recombinant AAV was also tested using beta-galactosidse expression. Cell viability was determined by using MTT assay to evaluate the toxicity of the transduction procedure. Expression and production of Insulin were tested using reverse transcriptase-polymerase chain reaction and immunocytochemistry. Secretion of human insulin and C-peptide from the cells was assayed using enzyme-linked immunosorbent assay. Production of insulin and C-peptide from the test group represented a higher increase compared to the control group. In this study, we examined generation of insulin-producing cells from mesenchymal stem cells by genetic engineering for diabetes therapy. This work might be valuable to the field of tissue engineering for diabetes treatment.

Bcl-2–associated athanogene 3 protects the heart from ischemia/reperfusion injury

Science.gov (United States)

Su, Feifei; Myers, Valerie D.; Knezevic, Tijana; Wang, JuFang; Gao, Erhe; Madesh, Muniswamy; Tahrir, Farzaneh G.; Gupta, Manish K.; Gordon, Jennifer; Rabinowitz, Joseph; Tilley, Douglas G.; Khalili, Kamel; Cheung, Joseph Y.

2016-01-01

Bcl-2–associated athanogene 3 (BAG3) is an evolutionarily conserved protein expressed at high levels in the heart and the vasculature and in many cancers. While altered BAG3 expression has been associated with cardiac dysfunction, its role in ischemia/reperfusion (I/R) is unknown. To test the hypothesis that BAG3 protects the heart from reperfusion injury, in vivo cardiac function was measured in hearts infected with either recombinant adeno-associated virus serotype 9–expressing (rAAV9-expressing) BAG3 or GFP and subjected to I/R. To elucidate molecular mechanisms by which BAG3 protects against I/R injury, neonatal mouse ventricular cardiomyocytes (NMVCs) in which BAG3 levels were modified by adenovirus expressing (Ad-expressing) BAG3 or siBAG3 were exposed to hypoxia/reoxygenation (H/R). H/R significantly reduced NMVC BAG3 levels, which were associated with enhanced expression of apoptosis markers, decreased expression of autophagy markers, and reduced autophagy flux. The deleterious effects of H/R on apoptosis and autophagy were recapitulated by knockdown of BAG3 with Ad-siBAG3 and were rescued by Ad-BAG3. In vivo, treatment of mice with rAAV9-BAG3 prior to I/R significantly decreased infarct size and improved left ventricular function when compared with mice receiving rAAV9-GFP and improved markers of autophagy and apoptosis. These findings suggest that BAG3 may provide a therapeutic target in patients undergoing reperfusion after myocardial infarction. PMID:27882354

Kak zhit v Jevropeiskom Sojuze? : ZAO "Ferteksos Transportas" = What will the life in the European Union be like? : The Fertexos Transportas private limited company / Dmitri Artamoshkin

Index Scriptorium Estoniae

Artamoshkin, Dmitri

2003-01-01

Transpordifirma "Fertexos" esindaja räägib ettevõtte transpordi - ja ekspedeerimisteenuste ning Leedu transpordisektori arendamisest arvestades territooriumi läbivaid rahvusvahelisi transpordikoridore ja Euroopa Liidu transpordipoliitika nõudeid

Mockus: Lithuanians work too much / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Välisleedulasest Colombia 2010. aasta presidendivalimiste kandidaat Antanas Mockus tuli Vilniusesse Demokraatiate Ühenduse kohtumisele. Ta kohtus Leedu välisministri Andronius Azubalisega ja vastas ajakirjanike küsimustele

Baltimaad Veneetsias / Kaili Kannel

Index Scriptorium Estoniae

Kannel, Kaili

2016-01-01

Eesti, Läti ja Leedu ühisprojekt "Balti Atlas" 15. Venezia arhitektuuribiennaalil. Biennaali üldteemaks on "Sõnumeid rindejoonelt", peakuraatoriks Tšiili arhitekt Alejandro Aravena, Eesti poolseks kuraatoriks Johan Tali

Kahtlased sõprussuhted = Dubious friendships / Ulrike Gerhardt

Index Scriptorium Estoniae

Gerhardt, Ulrike, 1984-

2014-01-01

Kohaspetsiifiline Läti, Leedu, Eesti, Saksamaa ja Rootsi ühisnäitus "(Re)construction of frendship" Riia Nurgamajas (endine riikliku julgeolekukomitee peakorter). Installatsiooniga "No borders" esines Tanel Rander Eestist

Туминас лечит себя и публику Чеховым / Ксения Репсон

Index Scriptorium Estoniae

Репсон, Ксения

2010-01-01

Lavastaja Rimas Tuminas ja Moskva Jevgeni Vahtangovi nimeline Teater toovad Tallinna Anton Tšehhovi "Onu Vanja". Lavastus kuulub teatrifestivali "Kuldne Mask Eestis" programmi. Artiklis leedu lavastajast, lavastusest ja teatrist

NATO harjutas Suwalki koridori kaitsmist

Index Scriptorium Estoniae

2017-01-01

Baltimaades rahvusvahelistel õppustel Saber Strike osalevad NATO väed harjutasid nädalavahetusel Suwalki koridori kaitsmist, mida liitlased saaksid kriisi korral kasutada Leedu, Läti ja Eesti toetamiseks

Though dramatic, 2003 was the "Year of Confirmation" / Gary Peach

Index Scriptorium Estoniae

Peach, Gary

2003-01-01

2003. aasta poliitilistest tähtsündmustest Baltimaades - euroreferendumitest Eestis, Lätis ja Leedus, Leedu presidendivalimistest, Riigikogu valimistest Eestis, Iraagi sõjast ja selle mõjust Balti riikidele

Eesti arhitektuur Pariisis

Index Scriptorium Estoniae

2000-01-01

Arhitektuurinäitus "4 Architectures" Pariisi arhitektuurigaleriis La Galerie d'Architecture. Näitus tutvustab Eesti, Läti, Leedu ja Rootsi uuemat arhitektuuri. Eestit esindab arhitektuuribüroo 3+1

Business full of optimism

Index Scriptorium Estoniae

2004-01-01

Ülevaade reklaamindusest Baltimaades - ideede leidmine, töötajate professionaalsus, turu kujunemine, integreeritud turunduse teenus jm. Tabelid: Eesti ja Leedu agentuuride reiting ; Töötajate arv reklaamiagentuurides 2003

Poles plan defense of RKN Orlen from hostile bid

Index Scriptorium Estoniae

2007-01-01

Poola majandusministeerium arendab plaani Leedu naftatöötlustehase Mazeikiu Nafta omaniku PKN Orleni kaitsmiseks võimalike Vene firmade vaenulike ülevõtmispakkumiste eest. Lisa. Divert from Eastern Europe?

Arunas Siksta : thinks regional, acts local / Arunas Siksta

Index Scriptorium Estoniae

Siksta, Arunas

2004-01-01

Lietuvos Telekomase juht olukorrast Leedu sideteenuste turul, Eesti Telefoni brändimuutusest, Lietuvos Telekomase ja Omniteli koostööst ja võimalikust ühinemisest ning ettevõtte turundustegevusest

The global armoured vehicles market / Dennis-P. Merklinghaus

Index Scriptorium Estoniae

Merklinghaus, Dennis-P.

2018-01-01

Ülevaade erinevate maailma riikide kaitsekulutustest, investeeringutest soomusmasinatesse ja sõjatehnikasse. Teiste Euroopa riikide hulgas on ka ülevaade Eesti, Läti ja Leedu kaitsekulutustest ja sõjatehnilistest uuendustest

Lithuania defends currency / Adam Mullett

Index Scriptorium Estoniae

Mullett, Adam

2009-01-01

Leedulased kiirustavad oma pangasäästusid vahetama eurode vastu, kuna kardetakse raha devalveerimist. Valitsus aga kinnitab, et vaatamata Leedu pangandussektoris esinevatele probleemidele pole lähiajal devalveerimist kavas

Letter from Little Lithuania / Michael Tarm

Index Scriptorium Estoniae

Tarm, Michael

2004-01-01

Autor teeb ülevaate Eesti, Läti, Leedu toodetest ja kaubamärkidest USA turul, Baltimaadesse puutuva kajastamisest USA meedias ning USA avalikkuse teadlikkusest Balti riikidest, sportlastest ja kultuuritegelastest

Adeno-associated viral vector serotypes 1 and 5 targeted to the neonatal rat and pig striatum induce widespread transgene expression in the forebrain

DEFF Research Database (Denmark)

Kornum, Birgitte R; Stott, Simon R W; Mattsson, Bengt

2010-01-01

Viral vector-mediated gene transfer has emerged as a powerful means to target transgene expression in the central nervous system. Here we characterized the efficacy of serotypes 1 and 5 recombinant adeno-associated virus (rAAV) vectors encoding green fluorescent protein (GFP) after stereotaxic...

Biological effects of rAAV-caAlk2 coating on structural allograft healing

DEFF Research Database (Denmark)

Koefoed, Mette; Ito, Hiromu; Gromov, Kirill

2005-01-01

Structural bone allografts often fracture due to their lack of osteogenic and remodeling potential. To overcome these limitations, we utilized allografts coated with recombinant adeno-associated virus (rAAV) that mediate in vivo gene transfer. Using beta-galactosidase as a reporter gene, we show...

LA NOTION DE TORTURE, DE TRAITEMENTS INHUMAINS ET DEGRADANTS DANS LA JURISPRUDENCE DE LA COUR EUROPEENNE DES DROITS DE L’HOMME

Directory of Open Access Journals (Sweden)

Jean-Paul Céré

2017-03-01

Full Text Available En vertu de l'article 3 de la Convention européenne de sauvegarde des droits de l'homme, les Etats ne peuvent ni pratiquer la torture ni infliger des traitements inhumains ou dégradants. Le particularisme de cette convention par rapport aux autres textes internationaux ou régionaux relatifs aux droits de l’homme réside indéniablement dans le système de protection existant qu’elle a organisé. Un organe juridictionnel, la cour européenne des droits de l’homme, est en charge de sanctionner les Etats qui ne respectent la Convention. Au terme d’une jurisprudence novatrice et évolutive, la cour européenne des droits de l’homme impose de plus en plus efficacement de lutter contre la torture, les traitements inhumains et dégradants.

Baleriini mitmekihiline elu : Boriss Eifmani ballett "Punane Giselle" / Maria Goltsman

Index Scriptorium Estoniae

Goltsman, Maria

2012-01-01

Balletist "Punane Giselle", mille süžee aluseks on vene baleriini Olga Spessivtseva elulugu, koreograafia ja libreto Boriss Eifman. Leedu Rahvusooperi külalisetendus Estonia teatris 28. septembril 2011

Elektrooniline teadusinfo Balti riikide ja Põhjamaade tehnikaülikoolide raamatukogudes / Kate-Riin Kont

Index Scriptorium Estoniae

Kont, Kate-Riin, 1971-

2010-01-01

Artiklis analüüsitakse teadusinfo kasutamist ja kulusid, samuti elektrooniliste ressursside kasutamisega seotud tulemusindikaatoreid ning võrreldakse olukorda Läti, Leedu, Soome, Rootsi ja Norra tehnikülikoolide raamatukogudega

Bridging Lithuania's energy gap / Adam Mullett

Index Scriptorium Estoniae

Mullett, Adam

2008-01-01

Leedu peab leidma võimalusi, kuidas leevendada pärast Ignalina tuumaelektrijaama sulgemist tekkivat energia defitsiiti. Võimalik on alternatiivenergia kasutamine, Rootsi ja Baltimaade vahelise elektrikaabli rajamine ning uue tuumaelektrijaama ehitamine

Dlja kompanii "Bega" ne nuzhen shljus v Jevrosojuz! = BEGA does not need any sluice to the EU / Aloizas Kuzmarskis

Index Scriptorium Estoniae

Kuzmarskis, Aloizas

2004-01-01

Leedu stividorettevõtte "Bega" juht Aloizas Kuzmarskis ettevõtte asutamisest, terminali ehitamisest koostöös Klaipeda meresadamaga, kaupade lastimise mahtudest, kvaliteedisertifikaatide omandamisest ja Euroopa Liiduga liitumise mõjudest sadamateenustele

Utility privatizations raise as many questions as cash / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2003-01-01

Leedu valitsus seiskab tõenäoliselt elektrijaotusvõrgu Rytu Skirstomieji Tinklai (RTS) erastamise. Teise võrgufirma Vakaru Skirstomieji Tinklai (VST) erastamiskomisjon on palunud konkursil osalejatelt täpsustatud pakkumisi

Politicians spin attacks against methadone, syringe replacement / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu parlamendiliikmed osalevad kampaanias, millega võideldakse narkomaanidele tasuta süstalde jagamise ja sõltuvuse vastase aine metadooni vastu, kuna nende arvates need meetmed pigem süvendavad narkomaaniat

Estonian ambassador ensures support for Ignalina extension

Index Scriptorium Estoniae

2008-01-01

Eesti saadik Vilniuses Andres Tropp kinnitas Eesti toetust Leedu püüdlustele pikendada Ignalina tuumareaktori töötamisaega. Einari Kisel majandusministeeriumist arvas, et sulgemine kiirendaks uue tuumajaama ehitust

Fluxus East / Petra Stegmann

Index Scriptorium Estoniae

Stegmann, Petra

2008-01-01

Näitusest "Fluxus East" Kumu Kunstimuuseumis. Fluxuse liikumisest leedu kunstniku George Maciunase (1931-1978) eestvedamisel. Liikumise ilmingutest Eestis (happeningid, muusikaaktsioonid, visuaalne poeesia, mail art). Kuraator Petra Stegmann, kujundaja Andrea Pichl

Kalnapilis will refresh its brand

Index Scriptorium Estoniae

2004-01-01

Leedu õlletööstuse Kalnapilis-Tauras Groupi turundusjuht Stig Mose Henriksen kavandatavatest muutustest ettevõtte turundusstrateegias, tootearendusest ja brändi maine parandamisest, oma eesmärkidest ettevõttes

Unit Link insurance product catches on in Latvia, finally

Index Scriptorium Estoniae

2005-01-01

Lätis tuleb turule uus elukindlustuse teenus, mille kaudu on võimalik kindlustuslepinguid siduda investeerimisfondidega. Eesti, Läti ja Leedu kindlustusturust. Tabel: Eesti elukindlustus ettevõtted : Eesti mitteelukindlustus ettevõtted

Prunskiene spurned for Kaliningrad visit / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu poliitikud avaldavad pahameelt põllumajandusminister Kazimiera Prunskiene Kaliningradi juubelipidustustele minemise üle, väites, et kui riigi presidenti pole kutsutud, siis ei tohiks ka keegi teine riiki esindada

Rakendav rahulolematus / Enn Soosaar

Index Scriptorium Estoniae

Soosaar, Enn, 1937-2010

2007-01-01

Autor arutleb parteide valijate üle Riigikogu valimistel ja leiab, et Riigikogu koosseis oleneb suurel määral sellest, kuivõrd suudavad erakonnad võita Res Publicas pettunud ja muidu rahulolematute valijate poolehoidu

Kes volitas? / Enn Vatter

Index Scriptorium Estoniae

Vatter, Enn

2009-01-01

Autor tõstatab küsimuse, kes volitas 20% töötajaskonnast hõlmava Tööandjate keskliidu ja 5-7% kaasava Ametiühingute keskliidu töölepinguseaduse küsimustes neisse institutsioonidesse mittekuuluva töötajaskonna, st. ligi 80% Eestis tööga hõivatuid esindama, tingimusi seadma, kokkuleppeid sõnastama ja sõlmima

Koolitaja kogemus / Enn Siim

Index Scriptorium Estoniae

Siim, Enn

2002-01-01

Vene koolide õpetajate täienduskoolitusprobleeme, integratsiooniprobleemide lahendamisest Kanada hariduses, humanistliku pedagoogika rakendamisest Tallinna Ümera Põhikoolis ja Venemaal Peterburi koolides. Eestis on loodud Humanistliku Pedagoogika Selts, humanistliku pedagoogika asjatundja Venemaal on pedagoogikadoktor professor Šalva Amonašvili

Pahaendeline pragmatism / Enn Soosaar

Index Scriptorium Estoniae

Soosaar, Enn, 1937-2010

2008-01-01

Vana Maailma kõlbeline kollaps oli see, mis lasi Euroopas tekkida ja võimust võtta XX sajandi totalitaarsetel režiimidel. Ohtu peame tajuma siis, kui huvide taha peidetakse riigi imperialistlikud ambitsioonid või merkantiilne saamahimu. Ilmunud ka Lääne Elu 18. okt. lk. 2

Põud : [luuletused] / Enn Tuuling

Index Scriptorium Estoniae

Tuuling, Enn

2002-01-01

Sisu: Põud ; Aian ; Inne pikset ; Süküsõdang tarõn ; Üü tulek ; Rebäse külä vana sann ; Vana maja ; "Kes oll̀ tuu miis, kes sinno ehit ..." ; Pööningul ; Talv 1960/61. Eluloolisi andmeid autori kohta lk. 358-359

Olympic Casino laieneb jõuliselt Lätis ja Leedus / Raigo Neudorf

Index Scriptorium Estoniae

Neudorf, Raigo

2003-01-01

Eesti juhtiv kasiinokett tungib Läti ja Leedu turule. Vilniuses avatakse Baltimaade suurim ja Põhjamaade üks suurimaid kasiinosid. Kommenteerib Eesti Hasartmängude Korraldajate Liidu direktor Tõnis Rüütel

Baltic death race 2004 / Julia Balandina

Index Scriptorium Estoniae

Balandina, Julia

2005-01-01

Leedu on Baltimaades autoõnnetuste arvu poolest esimesel kohal. Balti riikide teedeohutusega tegelevate ametnike sõnul on paljude liiklusõnnetuste põhjuseks halb liikluskultuur. Tabel: Hukkunute arv Baltimaades; Lätlaste hoolimatus

Commission : Paksas violated oath of office / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2003-01-01

Leedu presidendi ümber puhkenud skandaali uuriv Seimi erikomisjon leidis, et president Rolandas Paksas oli ja on oma sidemete tõttu haavatav ning see kujutab ohtu riigi julgeolekule. Lisa: Commission conclusions

Adamkus says security agencies should examine Brazauskas' family business / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu president Valdas Adamkus teatas oma kõnes, et peaminister Algirdas Barzauskase pereäri ümber tekkinud skandaali tagamaid ja süüdistusi peaksid uurima õiguskaitse institutsioonid, mitte poliitikud

Proposed Stalin statue causes outrage / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Teade Jossif Stalini uue kuju avamisest 9. mail Moskva pidustustel võib mõjutada Leedu presidendi Valdas Adamkuse otsust Moskvasse sõitmise suhtes. Lisa: Fakte riigijuhtide osalemise kohta 9. mail pidustustel

Mazeikiu refinery to stay in Yukos' control / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2004-01-01

Vene peaprokuratuur andis välja rahvusvahelise vahistamisorderi Mazheikiu Nafta juhatuse esimehe ja Jukose aktsionäri Mihhail Brudno suhtes. Leedu valitsus kaalub võimalikke samme, sealhulgas Mazheikiu Nafta aktsiate tagasiostmist

World Economic Forum discusses Lithuanian prospects / Nathan Greenhalgh

Index Scriptorium Estoniae

Greenhalgh, Nathan

2009-01-01

Maailma Majandusfoorumil pakuti välja lahendusi Leedu majandusliku olukorra parandamiseks. Üks meetod majanduskriisist väljumiseks oleks liti devalveerimine ning teine koos Läti ja Eestiga Baltimaade arengupanga loomine

Reda Marks : "Moi korol Mindaugas molodoi, silnõi i bez borodõ" / Eteri Kekelidze

Index Scriptorium Estoniae

Kekelidze, Eteri, 1944-

2003-01-01

Eesti Kunstiakadeemia nahakunstnikuna lõpetanud leedulanna Reda Marksi maalid kaunistasid Tallinna Matkamaja Leedu kuninga Mindaugase 750-nda kroonimisaastapäeva pidustuste ajal. Autori selgitusi kuningas Mindaugase porteele. Kunstnikust ja tema loomingust

Russians refuse cooperation, Lithuanians face dead-end / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu uurijad ei leidnud endise majandusministri Viktor Uspaskihhi kõrgharidust tõendava diplomi kohta informatsiooni, kuna Venemaal paikneva Plehhanovi Akadeemia töötajad keeldusid nendega koostööd tegemast

Veljo Tormisele Balti Assamblee kunstipreemia / Madis Kolk

Index Scriptorium Estoniae

Kolk, Madis

2000-01-01

Kunstipreemia - Veljo Tormis, kirjanduspreemia - läti poeet Janis Rokpelnis, teaduspreemia - leedu õpetlane Silvestras Gaizhiunas. Eesti kandidaatidele - V. Tormise kõrval Raoul Kurvitz, Jaan Undusk, Raimo Pullat - anti kõrge hinnang

A new leader for Lithuania's ruling party? / Kestutis Girnius

Index Scriptorium Estoniae

Girnius, Kestutis K.

2011-01-01

Irene Degutiene, kes on reitingult president Dalia Grybauskaite järel teine poliitik Leedus, tahab asendada erakonna Isamaaliit – Leedu Kristlikud Demokraadid praegust esimeest Andrius Kubiliust, kes on ka riigi peaminister

Saksa ettevõtete optimism jätkub

Index Scriptorium Estoniae

2014-01-01

Vaadeldes asukohamaaga seotud kriteeriume nii üksikult kui tervikuna, on Eesti, Läti ja Leedu - võrreldes teiste Ida- ja Kesk-Euroopa riikidega - siin tegutsevate saksa ettevõtete arvates hinnatud asukohamaad

Suddenly, Adamkus faces loud music / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu presidenti Valdas Adamkust kritiseeritakse põhjusel, et ta ei kutsunud riigi iseseisvuspäeva pidustustele 1990. aastal Iseseisvuse Aktile allakirjutanuid. Samuti süüdistatakse Presidendi Kantseleid riigilipu ebakorrektses heiskamises

House passes resolution on occupation

Index Scriptorium Estoniae

2005-01-01

Venemaalt Baltimaade okupeerimise tunnistamist nõudva resolutsiooni vastuvõtmisest USA Kongressi Esindajate Kojas Leedu päritolu kongressmani John Shimkuse eestvedamisel. Vt. ka resolutsiooni teksti "House Concurrent Resolution 128" lk. 14

Moscow, U.S. court move to block Mazeikiu deal, PKN Orlen offers record-high price

Index Scriptorium Estoniae

2006-01-01

USA kohus külmutas tehingud Vene naftakontsernile Jukos kuuluvate varadega Leedu naftatehases Mazeikiu Nafta. Tehas võib minna Jukose võlausaldaja Rosnefti valdusesse. Poola PKN Orlen pakkus 2 miljardit eurot

Politics and history: Lithuania and the Slavs / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Poliitilised ja ajaloolised suhted Leedu ja slaavlastest naaberrahvaste vahel. 15. novembril Delfi.lt-le antud intervjuus on vene filosoof Aleksandr Dugin öelnud, et Venemaa võib Baltimaid uuesti okupeerida. Kaart

Palga tõusuks jääb raha puudu / Airi Amtmann

Index Scriptorium Estoniae

Amtmann, Airi

2008-01-01

Paljud Raplamaa omavalitsused on probleemi ees, kuidas kindlustada pedagoogidele 22%-list palgatõusu. Arvamust avaldavad Vigala vallavanem Priit Kärsna, Käru vallavanem Elari Hiis, Rapla abivallavanem Imbi Kalberg, Kohila vallavanem Hillar Kalmet, Juuru vallavalitsuse haridus- ja kultuurispetsialist Aili Normak ja haridusministeeriumi nõunik Riho Raave

Pisarateta = No tears / Liivi Reinhold

Index Scriptorium Estoniae

Reinhold, Liivi

2016-01-01

Lauri Linnamäe ja Kaupo Kikkase fotoseeriast, kus poseerivad 16 naervat kaitseliitlast. Pildiseeria sündis vastuseks Leedu fotoseeriale pisaraid valavatest meestest armee välivormis, mis oli ajendatud ajateenistuse taastamisest

Kuidas kirjutada kunstiajalugu? / Kristina Jõekalda

Index Scriptorium Estoniae

Jõekalda, Kristina

2011-01-01

Kaunase ülikoolis 14. ja 15. oktoobril toimunud Eesti, Läti ja Leedu kunstiteadlaste konverentsist "(Un)Blocked Memory : Writing Art History in Baltic Countries" ("(Mitte)blokeeritud mälu : kunstiajaloo kirjutamine Baltimaadel")

The CIA shadow on the outskirts of Vilnius / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2009-01-01

Leedu võib olla esimene riik, kes tunnistab CIA vangla olemasolu oma territooriumil, kuna telekanali LNK TV andmetel tunnistasid seda mitmed ohvitserid. Poliitikutest, kes on vastanud küsimustele seoses CIA vanglaga

Print advertising in Lithuania / Arturas Olshauskas

Index Scriptorium Estoniae

Olshauskas, Arturas

2006-01-01

Leedu ajakirjanduse spetsialistid erinevate väljaannete juurest arutlevad sealse trükiajakirjanduse peamiste probleemide ja teemade üle. Tabelid: TOP 5 Lithuanian dailies; TOP 5 Lithuanian weeklies; TOP 5 Lithuanian monthlies

Investment climate of Lithuania and advanced ruling of investments / Linas V. Karlavicius, Birute Karlaviciene

Index Scriptorium Estoniae

Karlavicius, Linas V.

2003-01-01

Kaanel ajak. ilmumisaeg 2002/2003. Ülevaade välisinvesteeringute riiklikust regulatsioonist, dünaamikast, investoritest riikide lõikes ja ettevõtluskeskkonna mõjust välisinvestorite tegevusele Leedu näitel

Presidents forgive Polish no-show / Talis Saule Archdeacon

Index Scriptorium Estoniae

Archdeacon, Talis Saule

2007-01-01

30. oktoobril 2007 Riias kohtunud Läti president Valdis Zatlers, Leedu president Valdas Adamkus ja Eesti president Toomas Hendrik Ilves olid optimistlikud kolme riigi ja Poola koostöö osas tuumaelektrijaama projektis

A president's funeral / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Leedu endise presidendi Algirdas Brazauskase matustest 1. juulil 2010 Vilniuses võttis osa ka president Toomas Hendrik Ilves. Konfliktist katoliku kiriku esindajatega, kes ei tahtnud lubada Algirdas Brazauskase kirstu toomist Vilniuse katedraali

Putini küllakutse tekitab Tallinnale ja Vilniusele peavalu / Kai Kalamees

Index Scriptorium Estoniae

Kalamees, Kai

2005-01-01

Leedu ametlikud allikad kummutasid uudise president Adamkuse otsusest Moskva visiidist loobumise kohta. Baltimaade presidentide koostööst. Lisa: Poola taotleb MRP hukkamõistu. Vt. samas intervjuud presidendi sisenõuniku Mart Siimanniga

Hotel protests continue / Nathan Greenhalgh

Index Scriptorium Estoniae

Greenhalgh, Nathan

2009-01-01

Leedu Hotellide ja Restoranide Liidu president ei ole rahul praeguse majandusolukorraga, kus tõstetakse käibemaksu, puuduvad otselennud suurematesse Euroopa pealinnadesse ja tahetakse kärpida Euroopa kultuuripealinn 2009 projekti eelarvet

Mazeikiu avoids fine, gets new CEO

Index Scriptorium Estoniae

2007-01-01

Leedu naftatöötlustehase Mazeikiu Nafta uueks tegevjuhiks saab Marek Mroczkowski. Endine juht Paul Nelson English tasus võimaluse eest kodumaale naasta kautsjoni. Ettevõtet ähvardas trahv turul domineerimise eest

Foreign ministers highlight energy as national priority

Index Scriptorium Estoniae

2006-01-01

Leedu välisminister Antanas Valionis rõhutas energiapoliitika tähtsust välispoliitikas. Urmas Paeti sõnul peab EL väljendama selget liini energiapoliitikas ning Venemaa ratifitseerima Energia Harta

Baltic leaders who returned from West to East

Index Scriptorium Estoniae

2006-01-01

Artiklis tutvustatakse kolme Balti riigi presidentide sarnast minevikku eksiilis ning nenditakse, et varasem demokraatlikus riigis elamise kogemus on kindlasti kaasa aidanud Eesti, Läti ja Leedu majanduslikule ning välispoliitilisele edule

The last foreign visit for Kaczynski / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

10. aprillil juhtunud lennuõnnetuses hukkunud Poola presidendist Lech Kaczyńskist. Bronislaw Komorowski sai riigipea kohusetäitjaks. Lech Kaczyński 8. aprilli Leedu visiidist. Poola nimede kirjutamise probleemist Leedus

Pan-Baltic airline talks again on the table

Index Scriptorium Estoniae

2009-01-01

Baltimaad võivad luua ühise lennukompanii. Läti kommunikatsiooniministri Kaspars Gerhardsi, Leedu transpordiministri Eligijus Masiulise ja Eesti majandus- ja kommunikatsiooniministri Juhan Partsi arvamused. AirBaltic plaanib luua interneti-reisibürood

Agreement expected on moving nuclear project forward

Index Scriptorium Estoniae

2009-01-01

Leedu loodab allkirjastada Eesti, Läti ja Poolaga uue Ignalina tuumajaama ehituse deklaratsiooni 2010. aasta esimesel poolel. Konkursi abil kavatsetakse leida ka strateegiline investor, kes saab edaspidi üle poole Ignalina kasumist

Ei hoopiski päikeselisel Mauritiusel, vaid Eestis / Jaan Jürine

Index Scriptorium Estoniae

Jürine, Jaan, 1946-

2008-01-01

30. jaanuarist 2. veebruarini mängitakse Coral Clubi siseväljakuil FedCupi Euro-Aafrika tsooni teise liiga turniir. Eesti naiskonnale pakuvad konkurentsi Iirimaa, Kreeka Türgi, LAV, Bosnia ja Leedu

Adamkus, Kirkilas take Lithuania's energy case to East European partners

Index Scriptorium Estoniae

2008-01-01

Euroopa tuumaenergia foorumil esinedes palusid Leedu peaminister Gediminas Kirkilas ja president Valdas Adamkus EL-i partneritel taas kaaluda Ignalina tuumajaama lahtiolekuaja pikendamist. Hispaania ja Tšehhi esindaja ning Jose Manuel Barroso arvamused

Balti peaministrid unistavad kiirrongist / Toomas Sildam

Index Scriptorium Estoniae

Sildam, Toomas, 1961-

2003-01-01

Baltimaade peaministrite Algirdas Brazauskase, Einars Repshe ja Siim Kallase kohtumisest Kalvi mõisas. Arutlusest Helsingi-Berliini raudteeliini üle. Vt. samas lühiintervjuud Leedu peaministri Algirdas Brazauskasega: Brazauskas: Ignalina tulevik on kaalukausil

Latvia, Estonia differ on sugar

Index Scriptorium Estoniae

2004-01-01

Läti põllumajandusministri Martins Roze sõnul ei toeta Läti Euroopa Komisjoni reformikava suhkruturu liberaliseerimiseks, samal seisukohal näib olevat suhkrutootmisega tegelev Leedu. Eesti põllumajandusministeerium reformile vastu ei seisa

Tretii Pribaltiiskii Front / Vladimir Fridljand

Index Scriptorium Estoniae

Fridljand, Vladimir

2005-01-01

Eesti, Läti ja Leedu välisministrite kohtumisest Riias seoses presidentide otsusega Moskva 9. mai üritustes osalemise kohta. Balti riigid on tahtmatult avanud nn Teise maailmasõja kolmanda rinde, leiab autor

Extra Strong Super Light / Magdalena Lewoc

Index Scriptorium Estoniae

Lewoc, Magdalena

2003-01-01

2003. a. Szczecini Rahvusmuuseumis toimunud 5. rahvusvahelisest kunstinäitusest võtsid osa poola, saksa, rootsi, soome, leedu, läti, ukraina ja eesti kunstnikud. Pikemalt Ene-Liis Semperi ja Jaan Toomiku loomingust

Borisov not packing his bags yet

Index Scriptorium Estoniae

2004-01-01

Leedu kodakondsusest ilma jäetud ärimees Juri Borissov esitas migratsiooniametile taotluse Leedus alalise elamisloa saamiseks. Migratsiooniamet teeb lähipäevil otsuse Borissovi Leetu jäämise lubamise osas

Nuclear plant chaos

Index Scriptorium Estoniae

2010-01-01

Eesti peaminister Andrus Ansip leiab, et Läänemere regioonis on olnud liiga palju tuumajaama projekte ja Euroopa Liit peaks kaitsma energiaturgu ebaausa konkurentsi eest. Leedu ei ole Valgevene plaanitava tuumajaama asukohaga rahul

Tunne Kelam - kolleeg ja sõber / Christopher Beazley, Elmar Bork, Ari Vatanen ...[jt.

Index Scriptorium Estoniae

2009-01-01

Euroopa Parlamendi väliskomisjoni liikmed Christopher Beazley, Elmar Bork, Ari Vatanen, Leedu Vabariigi president Valdas Adamkus, Euroopa Parlamendi asepresident Alejo Vidal-Quadras ja Euroopa Parlamendi president Hans-Gert Pöttering Tunne Kelamist

Finance minister steps down / Arturas Racas

Index Scriptorium Estoniae

Racas, Arturas

2007-01-01

Leedu rahandusminister Zigmantas Balcytis teatas tagasiastumisest, et kaitsta oma poega spekulatsioonide eest. Balcytise poeg koordineerib Euroopa Liidu rahalise abi jagamist ning teda süüdistati ametiseisundi kuritarvitamises, kuid mõisteti hiljem õigeks

Lithuania 1997-1999 : Mirrors of Public Opinion of the Economic Situation / Vladas Gaidys

Index Scriptorium Estoniae

Gaidys, Vladas, 1952-

1999-01-01

Leedus aastatel 1997-1999 läbi viidud uuringu käigus selgitati rahvaaarvamus kahe küsimuse kohta: milline on Leedu praegune majanduslik olukord ja milline on teie perekonna majanduslik olukord. Diagrammid

Opinion polls show Lithuania's improving moods / Vladas Gaidys ; interviewed by Linas Jegelevicius

Index Scriptorium Estoniae

Gaidys, Vladas, 1952-

2011-01-01

Leedu turu-uuringute keskuse pikaaegne juht räägib leedulaste hoiakutest, avaliku arvamuse küsitlustest, parteidest, president Dalia Grybauskaitest, ekspresidentidest ja märgib, et uuringud ei ole ära ostetavad

Jalutuskäik Tallinna galeriides laupäeval, 17. XII / Juta Kivimäe

Index Scriptorium Estoniae

Kivimäe, Juta, 1952-

2005-01-01

Jaan Elkeni ja Anu Põdra ühisnäitus "Mina ja tema" Deco galeriis kuni 30. XII. Kristin Kalamehe näitus "Mehed" Tallinna Linnagaleriis kuni 1. I 2006. Alice Kase joonistused Hobusepea galeriis 13.-19. XII. Rita Raave näitus "Vana sein" G-galeriis kuni 31. XII

Adamkus, Yushchenko discuss energy cooperation / Kimberly Kweder

Index Scriptorium Estoniae

Kweder, Kimberly

2007-01-01

Leedu president Valdas Adamkus ja Ukraina president Viktor Jushtshenko kohtusid Krimmis. Peateemaks olid energiaküsimused. Ukraina plaanib ehitada energiakoridori, mis ühendaks Kaspia merd ja Euroopat. Ukraina võib liituda tuumaelektrijaama projektiga Leedus

Energy leads talks

Index Scriptorium Estoniae

2011-01-01

Leedu energeetikaminister Arvidas Sekmokas lubab teha kõik endast sõltuva, et Leedus hakataks põlevkivigaasi tootma. Baltimaade peaministrid ei ole suutnud siiani otsustada, millisesse riiki tuleb EL-i poolt osaliselt finantseeritav LNG-terminal

Vaja on professionaalseid oskusi! / Tiina Lokk ; interv. Maria Ulfsak

Index Scriptorium Estoniae

Lokk, Tiina, 1955-

2004-01-01

Eesti poolt Leedu teleprogrammide ja -filmide festivalil "Amber arch" žüriis osalenud T. Lokk nähtud filmidest ja peapreemia võitnud Priit Valkna dokumentaalfilmist "Hunt", mille tootis Allfilm ETV "Eesti lugude" dokumentaalsarja

Projekti Min-E-Max kohtumine Saksamaal / Katrin Arge

Index Scriptorium Estoniae

Arge, Katrin

2005-01-01

Oktoobri keskel toimus Lõuna-Saksamaa väikelinnas Oberderdingenis Hispaania, Portugali, Ungari, Leedu, Eesti ja Saksamaa õpetajate kohtumine seoses Euroopa Liidu poolt rahastatava Comenius1 projektiga Min-E-Max (minimum energy input maximum output)

Римас Туминас: надо убить своего зрителя! / Римас Туминас ; интервьюировал Николай Караев

Index Scriptorium Estoniae

Туминас, Римас, 1952-

2010-01-01

Anton Tšehhovi "Onu Vanja" teatrifestivalil "Kuldne Mask Eestis", lavastaja Rimas Tuminas, Moskva Jevgeni Vahtangovi nimelise Teatri kunstiline juht. Intervjuus "Onu Vanja" lavastusest, leedu lavastajatest, lavastajatöö meetoditest, oma teatrist, näitlejatest

Nuclear project back on track / Matt Garrick

Index Scriptorium Estoniae

Garrick, Matt

2011-01-01

1. juuniks on Leedu valitsus saanud Visaginasesse rajatava tuumaelektrijaama puudutavad investeerimisettepanekud Hitachi-GE Nuclear Energy Limited, General Electric Co. ja Westlinghouse Electric Company poolt. Peaminister Andrius Kubiliuse sõnul toob tuumaelektrijaam Leetu energiasõltumatuse

Mezhpravitelstvennoje soglashenije mezhdu Estonijei i Latvijei / Kristina Kareva

Index Scriptorium Estoniae

Kareva, Kristina

2006-01-01

Siseminister Kalle Laanet viibis Leedus, et arutada Läti siseministri Dzintars Jaundzheikarsiga ja Leedu siseministri Gintaras Furmanaviciusega ettevalmistusi Schengeni õigusruumiga liitumiseks ning sõlmida Eesti ja Läti vaheline politsei koostöölepe

"Central Station" Londonis

Index Scriptorium Estoniae

2000-01-01

Londoni galeriis Milch seitsme läti, leedu ja eesti kunstniku projekt "Central Station". Kuraatorid Lisa Panting, Sally Tallant. Eestist osalevad Hanno Soans (Catarina Campinoga koostöös valminud video), Kiwa, Kai Kaljo

Environment minister faces no-confidence proceedings / Kimberly Kweder

Index Scriptorium Estoniae

Kweder, Kimberly

2007-01-01

Leedu opositsiooniparteid väljendasid umbusaldust keskkonnaminister Arunas Kundrotase vastu, saates talle arupärimise küsimused EL-i fondide jagamise, jäätmekäitluse, vee ja õhu kvaliteedi jm. kohta

Lithuanian chemical industry remains profitable / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2009-01-01

Leedu keemiatööstus, mis on ikka veel kasumlik, moodustab 16,5% riigi SKP-st. Poola Orlen ostis peaaegu 90% Mazeikiu Nafta aktsiatest. Ainus Baltimaade naftatöötlemistehas asub Leedus. Farmaatsiaettevõtetest

Passions over the economy minister / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Koolide remondiprojektiga seoses nõudis Leedu president Dalia Grybauskate majandusminister Dainius Kreivyse tagasiastumist, kuna projekti pidi teostama ettevõte, mille osanikuks on ka majandusministri ema. 8. märtsil teatas Dainius Kreivys oma tagasiastumisest

VP Market teeb rekordkasvu / Ralf-Martin Soe

Index Scriptorium Estoniae

Soe, Ralf-Martin

2006-01-01

Ilmunud ka: Delovõje Vedomosti 26. apr. lk. 4. Leedu omanikele kuuluv T-Marketite poekett teeb Eestis rekordilist käibe kasvu. Diagramm. Vt. samas: Raimo Ülavere. VP Market annab konkurentidele silmad ette

Moda zajavljajet o sebe / Anna Korjakina

Index Scriptorium Estoniae

Korjakina, Anna

2005-01-01

Balti Moeföderatsiooni organiseeritud Riia moenädalast, kus oma töid demonstreerisid Läti, Eesti, Leedu, Venemaa ja Prantsusmaa moeloojad. Eestist Oksana Tandit ja Triinu Pungits. Pikemalt Masha Tsigali kollektsioonist (Venemaa)

Eluruumi üüriõiguse võrdlevõiguslik analüüs [Võrguteavik] / Ave Hussar

Index Scriptorium Estoniae

Hussar, Ave, 1971-

2015-01-01

Üürileandja varaliste huvide kaitsest ning üürniku sotsiaalsete ja varaliste huvide kaitsest. Võrreldakse Eesti eluruumi üürilepingute õiguslikku regulatsiooni Läti, Leedu, Soome, Rootsi, Saksa ja Šveitsi vastava õigusega

New generation e-passports "come online" - with digital photo, dactylogram and more / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu diplomaadid saavad masinloetavad passid, mis muudab piiriületamise kiiremaks ja turvalisemaks. Euroopa Liidu regulatsiooni kohaselt peavad kõik liikmesriigid olema valmis 2006. aasta sügiseks kasutusele võtma biomeetrilise informatsiooniga reisidokumendid

Naised valikute ees / Ave Paavo

Index Scriptorium Estoniae

Paavo, Ave

2006-01-01

Mõedaku spordibaasi juhataja Anu Kilk, äritarkvarafirma Oracle Eesti, Läti, Leedu finantsjuht Anne Oja-Aru, restoranide Balthasar, Karl Friedrich, Paat ja trahteri Postipoiss üldjuht Ritta Roosaar oma tööalastest valikutest

On potential enemies and potential targets / Egdunas Racius ; interv. Sven Becker

Index Scriptorium Estoniae

Racius, Egdunas

2006-01-01

Araabia regiooni tundev spetsialist annab intervjuus ülevaate olukorrast Iraagis, vastates küsimustele, mis puudutavad riigis demokraatia üles ehitamist, terrorismiga toimetulemist ning Leedu sõjaväelaste viibimist Iraagis. Lisa: Egdunas Racius

Balti riikide presidendid tänasid Bushi toetuse eest / Sten A. Hankewitz

Index Scriptorium Estoniae

Hankewitz, Sten A.

2002-01-01

Vilniuses USA presidendi George W. Bushiga kohtunud Eesti president Arnold Rüütel, tema Läti kolleeg Vaira Vike-Freiberga ja Leedu riigipea Valdas Adamkus tänasid Bushi toetuse eest NATO-kutse saamisel

Populismi kahe teraga mõõk / Mari-Liis Jakobson

Index Scriptorium Estoniae

Jakobson, Mari-Liis, 1985-

2012-01-01

Tallinna Ülikooli riigiteadlased koos Läti ja Leedu uurijatega viisid läbi uuringu populismist Baltikumis, keskendudes viimati toimunud valimistele. Artikli autor on Tallinna Ülikooli üldpolitoloogia lektor, uuringu "Populism Baltikumis" koordinaator

An amplified promoter system for targeted expression of calcium indicator proteins in the cerebellar cortex

Directory of Open Access Journals (Sweden)

Bernd eKuhn

2012-07-01

Full Text Available Recording of identified neuronal network activity using genetically encoded calcium indicators (GECIs requires labeling that is cell type-specific and bright enough for the detection of functional signals. However, specificity and strong expression are often not achievable using the same promoter. Here we present a combinatorial approach for targeted expression and single-cell-level quantification in which a weak promoter is used to drive trans-amplification under a strong general promoter. We demonstrated this approach using recombinant adeno-associated viruses (rAAVs to deliver the sequence of the GECI D3cpv in the mouse cerebellar cortex. Direct expression under the human synapsin promoter (hSYN led to high levels of expression (50-100 µM in five interneuron types of the cerebellar cortex but not in Purkinje cells (PCs (≤10 μM, yielding sufficient contrast to allow functional signals to be recorded from somata and processes in awake animals using two-photon microscopy. When the hSYN promoter was used to drive expression of the tetracycline transactivator (tTA, a second rAAV containing the bidirectional TET promoter (Ptetbi could drive strong D3cpv expression in PCs (10-300 µM, enough to allow reliable complex spike detection in the dendritic arbor. An amplified approach should be of use in monitoring neural processing in selected cell types and boosting expression of optogenetic probes. Additionally, we overcome cell toxicity associated with rAAV injection and/or local GECI overexpression by combining the virus injection with systemic pre-injection of hyperosmotic D-mannitol, and by this double the time window for functional imaging.

High value FDI requested / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2009-01-01

Otsestest välisinvesteeringutest Leedus. Suurbritannia pank Barclays loob Vilniusesse IT-keskuse. 4. septembril kirjutasid Leedu president Dalia Grybauskaite ja Prantsusmaa president Nicolas Sarkozy alla kahe riigi vahelise strateegilise partnerluse- ja koostööleppe

Die Baltikum-Charta vom 23. August 1979 und der KGB: Sieger und Verlierer / Ritvars Jansons

Index Scriptorium Estoniae

Jansons, Ritvars

2010-01-01

23. augustil 1979 avalikustatud 45 Eesti, Läti ja Leedu kodaniku avalikust kirjast, milles nõuti Molotovi-Ribbentropi pakti salajaste lisaprotokollide avalikustamist ning õigustühiseks kuulutamist allakirjutamise hetkest. Märgukirja hilisem nimetus Balti Apell

Arnold Rüütel: "Kohati jääb meie erakondadel puudu sisemisest demokraatiast." / Arnold Rüütel ; intervjueerinud Urmo Soonvald

Index Scriptorium Estoniae

Rüütel, Arnold, 1928-

2004-01-01

President Arnold Rüütel vastab küsimustele, mis puudutavad vedurijuhtide streiki, noorte arstide lahkumist välismaale, Rahvaliidu saadikurühma tekkimist Tallinna volikogus, Res Publica ja Reformierakonna võimalikku liitumist, Leedu-visiiti

Lithuania : corporate spying a threat to national security

Index Scriptorium Estoniae

2008-01-01

Leedu majandusministeeriumi poolt avaldatud majandusliku turvalisuse arendamise programmi järgi on tööstusspionaaž Leedule suurimaks ohuks. Samuti on majanduslikult ohtlik sõltumine Venemaa energiaturust ning Skandinaavia riikide investeeringute suur osakaal panganduses

Balti riikide raamatukoguhoidjate avaldus pagulastele pakutavate raamatukoguteenuste asjus / inglise keelest tõlkinud Tiina Büttner

Index Scriptorium Estoniae

2015-01-01

Avalduse koostasid Läti, Eesti ja Leedu raamatukoguhoidjate ühingud ning selle võtsid ühehäälselt vastu Balti raamatukoguhoidjate 10. kongressi ning Läti raamatukoguhoidjate 17. konverentsi osalejad Riias 13. oktoobril 2015

Paksas' acquittal reversed

Index Scriptorium Estoniae

2005-01-01

Apellatsioonikohus mõistis Leedu endise presidendi Rolandas Paksase riigisaladuse lekitamises süüdi, mis on vastupidine Vilniuse Kohtu otsusele. Ekspresidendile ei määratud vanglakaristust, kuid ta peab maksma 2715 euro suuruse trahvi

Peeter Allik, Urmas Viik jt Londonis

Index Scriptorium Estoniae

2006-01-01

Eesti, Läti ja Leedu graafikute näitus "Power and Man. Three Countries. One Exhibition" galeriis Novas Gallery. Eestist osalevad Peeter Allik, Urmas Viik, Jaak Visnap, Kadri Alesmaa, Aili Vahtrapuu, Inga Heamägi

Tšetšeenid, Leivo ja Austria / Priit Hõbemägi

Index Scriptorium Estoniae

Hõbemägi, Priit, 1957-

2004-01-01

Eesti, Läti, Leedu ja Austria siseministrid esitasid ühise idee rajada Ukrainasse tšetšeeni põgenikele pagulaslaager. Autor selgitab, mis huvid võisid olla Eesti ja Austria siseministritel sellise idee esitamisel

Funeral for business empire's owner / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

Leedu Töösturite Konföderatsiooni president ja Achema Group'i omanik Bronislovas Lubys suri Druskininkai kuurordis 23. oktoobril. Tema matustele oli tulnud ka peaminister Andrius Kubilius. Bronislovas Lubys'e poliitilistest ja majanduslikest seisukohtadest

Brazauskas address doesn't live up to expectations / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu peaminister Algirdas Brazauskas pidas oma televisiooniesinemises tema vastu esitatud süüdistusi õigustamatuteks. Nii koalitsioon kui opositsioon kritiseerisid peaministri kõnet, kuna ta ei vastanud varem esitatud küsimustele, vaid pidas monoloogi

"Tuumajaam on nagu beebi, keda tuleb üheksa kuud kanda" / Arturas Jonkus ; interv. Toivo Tänavsuu

Index Scriptorium Estoniae

Jonkus, Arturas

2008-01-01

Leedu rahvusliku energiafirma LEO LT avalike suhete juhi väitel edeneb uue tuumaelektrijaama projekt kenasti, valminud on tasuvusuuring, veebruaris lõpetatakse keskkonnamõjude analüüs, alustatud on logistika analüüsi

Ugly details of MP corruption come to light / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2004-01-01

Leedu Seimi korruptsioonivastase võitluse komisjon otsustas avalikustada parlamendiliikmete Vytenis Andriukaitise, Vytautas Kvietkauskase ja Arvydas Vidziunase telefonikõnede lindistused, mis tõendavad, et nad võtsid altkäemaksu kontserni Rubicon Group juhilt Andrius Janukoniselt

Margus Pärn : turuliidri koht on Maximale jõukohane 4-5 aastaga / Margus Pärn ; interv. Viljar Rääsk

Index Scriptorium Estoniae

Pärn, Margus

2008-01-01

Leedu jaekaubandusketi Maxima Eesti juht Margus Pärn vastab küsimustele, mis puudutavad Eesti jaekaubandusturgu ja Maxima positsiooni selles, ettevõtte laienemist ning turuliidri rolli saavutamise võimalust. Lisa: Margus Pärn. Tabel: Maxima

Edukus jättis eurost ilma / Sirje Rank

Index Scriptorium Estoniae

Rank, Sirje, 1966-

2006-01-01

Euroopa Komisjon lükkas Eesti ja Leedu taotlused eurotsooniga liitumiseks tagasi. Küsimustele vastab Taani majandusprofessor Niels Thygesen. Diagramm: EL-i riikide majanduskasv. Vt. samas: Eurotsooni praeguste liikmete jaoks on reegleid ümber kirjutatud

Vozmozhnõ variantõ / Eteri Kekelidze

Index Scriptorium Estoniae

Kekelidze, Eteri, 1944-

2008-01-01

Energiapoliitika seisukohalt on oluline Baltimaade ühise energiavõrgustiku loomine, mis tagab Eesti, Läti ja Leedu varustamise vajaliku elektrienergiaga, selle realiseerimiseni aga tuleb jätkata seniste energiatootjate moderniseerimist, tõdeb professor Hanon Barabaner

Läti Rahvusliku Kunstimuuseumi hoone uuendamisest / Margit Mutso

Index Scriptorium Estoniae

Mutso, Margit, 1966-

2010-01-01

Läti Rahvusliku Kunstimuuseumi (saksa arhitekt Wilhelm Neumann) rekonstrueerimise ja laienduse rahvusvahelisest avalikust arhitektuurivõistlusest. 1. koha pälvinud Leedu arhitektuuribüroo Processoffice ja 2. koha saanud Eesti arhitektuuribüroo Salto lahendustest

Kirkilas threatens to quit / Kimberly Kweder

Index Scriptorium Estoniae

Kweder, Kimberly

2007-01-01

Leedu peaminister Gediminas Kirkilas ähvardas loobuda ametikohast, kui parlament hääletab seaduseeelnõu poolt, mis lubab välja anda sadade miljonite littide väärtuses maksmata pensione, destabiliseerides riigi majandust

Real exchange rate adjustment in European transition countries / Florin G Maican, Richard J Sweeny

Index Scriptorium Estoniae

Maican, Florin G

2005-01-01

Reaalkursi hindamise ühikjuure testi (unit-root test) tulemustest kümnes (Tšehhi, Eesti, Ungari, Läti, Leedu, Poola, Slovakkia, Sloveenia, Bulgaaria, Rumeenia) Kesk- ja Ida-Eroopa siirdemajandusega riigis ajavahemikul 1993-2003. Graafikud. Tabelid

Adeno-associated viral vector serotypes 1 and 5 targeted to the neonatal rat and pig striatum induce widespread transgene expression in the forebrain

DEFF Research Database (Denmark)

Kornum, Birgitte R; Stott, Simon R W; Mattsson, Bengt

2010-01-01

. Our results show that striatal delivery of rAAV5 vectors in the neonatal brain represents a useful tool to express genes of interest both in the basal ganglia and the neocortex. Furthermore, we apply, for the first time, viral vector-mediated gene transfer to the pig brain providing the opportunity...

Saku õlu... otse Klaipedast? / Ede Schank

Index Scriptorium Estoniae

Schank, Ede

2005-01-01

Baltic Beverages Holdings'ile kuuluva Saku õlletehase osa eestikeelsete etikettidega pudelitest täidavad läti ja leedu pruulijad. Lisa: Saku on Baltika kõrval poisike. Vt. samas: Krister Kivi. Inglased "vallutavad" Viru õllega maailma

MPs vote to investigate PM Brazauskas' family business / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu parlament hääletas uurimiskomisjoni loomise poolt, mis hakkab uurima peaminister Algirdas Brazauskase perekonna äritegevust. Peaminister on varem teatanud, et ei anna tunnistusi, kui selline komisjon luuakse ning võib isegi tagasi astuda

Former health care vice minister sentenced / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Leedu endise tervishoiu aseministri Arturas Skikase kohtuotsusest seoses altkäemaksu võtmisega. Arturas Skikas tunnistas üles, et soovis saada verekeskuse direktori käest 5800 eurot. Tervishoiuminister Algis Caplikas jääb ametisse

Paksas declares candidacy amid uncertainty / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2004-01-01

Leedu ametieetika peakomisjon otsustas, et ametist tagandatud ekspresident Rolandas Paksas ei saa kolm aastat riigiametites töötada. See otsus võib takistada Paksase kandideerimist ennetähtaegsetel presidendivalimistel. Vt. samas: And here's the competition...

Baltics to host international war games

Index Scriptorium Estoniae

2004-01-01

USA armee juhtimisel juulis 2004 Balti riikides toimuvatest sõjalistest ja terrorismivastase võitluse ühisõppustest, kus osalevad Eesti, Läti, Leedu, Saksamaa, Poola, Rumeenia, Moldova, Ukraina, Gruusia, Armeenia ja Aserbaidžaani üksused

Brussels talks about finances, Libya and nukes / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

24.-25. märtsil Brüsselis toimunud Euroopa Ülemkogust osavõtnud Leedu president Dalia Grybauskaite rääkis Valgevenesse rajatavast tuumajaamast ja selle ohust. Kohtumisel kiideti heaks ka stabiilsusmehhanismi ja euroala pakt

McCain visits Vilnius to discuss Belarus / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2011-01-01

USA senaatorite ja Esindajatekoja delegatsiooni Leedu visiidi käigus kohtus John McCain Leedus õppivate Valgevene üliõpilaste ja president Dalia Grybauskaitega, et rääkida Valgevenes toimuvast. Leedus toimunutest Valgevene temaatilistest konverentsidest

The Kubilius government survives major opposition attack / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2010-01-01

Leedu transpordi- ja kommunikatsiooniministri Eligijus Masiulise umbusaldus ebaõnnestus. Peaminister Andrius Kubilius leiab, et opositsioon soovis sellega tema juhitud valitsust kukutada, kuna Eligijus Masiulist peetakse valitsuse parimaks ministriks. Euroopa Parlamendi liikme Roland Paksase poolt alustatud kohtuasjast

Ja polagaju, tshto vsjo sledujet shit! / Anna Korjakina

Index Scriptorium Estoniae

Korjakina, Anna

2005-01-01

31.03-3.04 toimus Riias moenädal - Riga Fashion Show. Oma töid demonstreerisid Läti, Eesti, Leedu, Venemaa ja Prantsusmaa moeloojad. Eestist Oksana Tandit ja Triinu Pungits. Pikemalt Masha Tsigali kollektsioonist (Venemaa)

Conscription debate in Lithuania : how to approach the issue? / Tomas Jermalavicius

Index Scriptorium Estoniae

Jermalavičius, Tomas, 1977-

2002-01-01

Autor teeb sõjaväekohustuse ajaloolise kujunemise ja lääneriikides levivate trendide taustal ülevaate peamistest argumentidest ning seisukohtadest Leedu ühiskonnas toimuvas debatis sõjaväekohustuse võimaliku kaotamise üle

Baltimaade rahvusraamatukogude direktorid kirjutasid alla koostööleppele / Annika Koppel

Index Scriptorium Estoniae

Koppel, Annika, 1964-

2014-01-01

23. aprillil 2014 kirjutasid Eesti Rahvusraamatukogu peadirektor Janne Andresoo, Läti Rahvusraamatukogu direktor Andris Vilks ja Leedu Rahvusraamatukogu peadirektor Renaldas Gudauskas alla koostööd tihendavale memorandumile. Artiklile on lisatud koostöömemorandumi tekst

Laenukasv tõstis Hansa Capitali kasumit 80% / Kaja Koovit

Index Scriptorium Estoniae

Koovit, Kaja, 1968-

2001-01-01

Laenunõudluse suurenemise tulemusena teenis EGO kaarti pakkuv Hansa Capital Grupp (HCG) mullu 395 mln krooni puhaskasumit. Tänavu oodatakse suurimat kasvu Läti ja Leedu liisingu- ja faktooringuturul. Tabel: Hansa Capitali majandusnäitajad paranevad

PM : Cabinet likely to choose TNK-BP

Index Scriptorium Estoniae

2005-01-01

Tõenäoliselt saab Mazeikiu Nafta aktsiate ostjaks Suurbritannia-Vene ettevõte TNK-BP. Endiselt soovib ka Leedu Jukose osa naftakompaniist osta, kuid selleks raha laenamine võib mõjutada riigi majandust ja üleminekut eurole

Government promises billions for highways / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2004-01-01

Läti transpordiministeerium avaldas riigi teedevõrgu arengukava järgmiseks 25 aastaks, mille kohaselt investeeritakse maanteede ja raudteede ehitusse kuni 18 miljardit litti. Leedu transpordiettevõtjate hinnangul ei ole uued suuremahulised projektid majandusarengu seisukohalt vajalikud

CIA vedas terroriste Pärnu kaudu Leedu salavanglasse? / Urmas Kaldmaa

Index Scriptorium Estoniae

Kaldmaa, Urmas

2009-01-01

Inimõiguste kaitse organisatsiooni Human Rights Watch väitel on USA luure keskagentuuri lennuk Boeing 737 kolmel korral maandunud Pärnu lennuväljal ning sama lennukiga vedas CIA terrorismis kahtlustatavaid Euroopasse, Euroopast Afganistani, Iraaki jt. riikidesse

Gutters lesing på nett : lesing og motivasjon

OpenAIRE

Sætersmoen, Margrethe

2010-01-01

Mastergradsoppgave i språk, kultur og digital kommunikasjon, Avdeling for lærerutdanning og naturvitenskap, Høgskolen i Hedmark, 2010 Norsk: Det overordnede temaet for min avhandling er ungdomsskolegutters motivasjon for lesing av digitale tekster. Mye av guttenes lesing på fritiden, foregår på skjerm. Hver dag leser de ut fra mange forskjellige motiv, likevel viser store leseundersøkelser som PISA og PIRLS at unge gutter leser mindre enn jenter, de leser dårligere enn jenter, og er mindre...

Report raises questions about Ignalina shutdown

Index Scriptorium Estoniae

2004-01-01

Balti riikide, Valgevene ja Venemaa energiafirmade juhid saatsid Leedu peaministrile Algirdas Brazauskasele kirja, milles märgivad, et viie riigi energiatööstus ei oleks peale Ignalina tuumajaama esimese reaktori sulgemist enam usaldusväärne

"Вышгород" - на стендах "Русского Зарубежья" / Людмила Глушковская

Index Scriptorium Estoniae

Глушковская. Людмила, 1937-

2006-01-01

Moskvas toimus 23.-27. oktoobrini a-tel 1991-2006 ilmunud venekeelse humanitaarteadusliku kirjanduse näitus "Üle piiride : venekeelne sõna Eesti, Läti ja Leedu teaduses ja kirjanduses". Ka Võshgorodi toimetuse "portfelli" täienemisest

Meie mees Reykjavikis - see, kes julges / Rein Veidemann

Index Scriptorium Estoniae

Veidemann, Rein, 1946-

2015-01-01

Dokumentaalfilmist "Need, kes julgevad", režissöör Ólafur Rögnvaldsson (Island-Eesti-Läti-Leedu 2015) ETV-s. Film Baltimaade iseseisvumisest ja Islandi tolleaegsest välisministrist Jón Baldvin Hannibalssonist selles protsessis

Presidents bid farewell to Vike-Freiberga / Talis Saule Archdeacon

Index Scriptorium Estoniae

Archdeacon, Talis Saule

2007-01-01

Eesti president Toomas Hendrik Ilves ja Leedu president Valdas Adamkus jätsid 3. juulil 2007 Riias hüvasti ametist lahkuva Läti presidendi Vaira Vike-Freibergaga. Baltimaade liidrid arutasid koostöö küsimusi

Triennaali peaauhind läks Leetu

Index Scriptorium Estoniae

2004-01-01

Tallinna XIII graafikatriennaalil väljaantud preemiad: peaauhind - leedu graafik Egle Kuckaite, diplomid - eesti kunstirühmitus John Smith (projekt betoonkohvritega "100 John Smithi") ja Eve Kiiler (fotoseeria "Fantastiline realism"), kiituskiri - eesti rühmitus Anonymous Boh

Sündinud vajadusest õhu ja hingamise järele / Jukka Pennanen, Mace Ojala ; soome keelest tõlkinud Triinu Seppam

Index Scriptorium Estoniae

Pennanen, Jukka

2012-01-01

"Jalgrattasõit raamatukogude eest Balti iikides 2012 ("Cycling for Libraries in the Baltic States 2012") toimus 28. juulist 7. augustini 2012, kus erinevate maade raamatukoguhoidjad ja raamatusõbrad külastasid Eesti, Läti ja Leedu raamatukogusid

Via Hanseatica luubi all / Andres Loorand

Index Scriptorium Estoniae

Loorand, Andres, 1969-

2006-01-01

Jõgeval toimunud seminaril tutvustati saavutusi Baltimaade turismivõrgustiku väljatöötamisel Via Hanseatica arenguvööndis. Via Hanseatica maantee ulatub Balti riikides 688 km Panemunest (Leedu) Narvani, projekti koordineerib sihtasutus Lõuna-Eesti Turism

31. VIII avati Vaala galeriis...

Index Scriptorium Estoniae

2004-01-01

Näitus "Märkmed maalist. Eesti ja Leedu noor kunst". Kuraatorid Margaret Tali ja Milda Zvirblyte. Osalevad Maarit Murka, Tõnis Saadoja, Kristi Laanemäe, Vilius Vanagas, Agile Mazonaite, Jolanta Kyzikaite, Ieva Baranauskaite. Kõlab Taavi Laatsiti originaalmuusika

Leo Burnett wins agency of the year title in Estonia and Lithuania

Index Scriptorium Estoniae

2004-01-01

Eesti Kuldmuna reklaamikonkursil, Leedu reklaamifestivalil Adrenalinas aasta reklaamiagentuuri tiitli pälvinud ettevõttest Kontuur Leo Burnett. Madis Laas, Tomas Ramanauskas ja Marius Petrukaitis vastavad küsimustele firma edu võimalike põhjuste ja tulevikuplaanide kohta

Kavaldavad kuraatorid ja noor kunst / Teet Veispak

Index Scriptorium Estoniae

Veispak, Teet, 1955-

2007-01-01

Eesti ja Leedu noorte maalikunstnike ühisnäitus "Fresh Paint" Vaal-galeriis. Eksponeeritakse Elis Saarevälja, Alvar Reisneri, Sirli Heina, Triinu Lille, Rait Rosina, Merike Estna töid. Kuraatorid Maarin Ektermann ja Milda Zhvirblyte

Usackas reveals future foreign policy goals / Adam Mullett

Index Scriptorium Estoniae

Mullett, Adam

2008-01-01

Tulevane Leedu välisminister Vygaudas Usackas teatas, et riigi välispoliitika on tulevikus suunatud Euroopa Liidu ja NATO liikmelisusele ning koostööle naaberriikide ja USA-ga. samuti kavatseb Usackas muuta poliitikat Venemaa suunal

Copenhagen, December 2002 - the terms fixed at last

Index Scriptorium Estoniae

2003-01-01

Kokkuvõte EL-i Kopenhaageni tippkohtumisest 2002. aasta detsembris, liitumisläbirääkimiste lõpptulemustest Eesti, Läti ja Leedu jaoks. Kommenteerib Alar Streimann. Tabel. Vt. samas: The EU and Russia reached compromise over Kaliningrad

Parliamentary elites as a democratic thermometer : Estonia, Lithuania and Moldova compared / William E. Crowther, Irmina Matonyte

Index Scriptorium Estoniae

Crowther, William E.

2007-01-01

Uuringutulemused Eesti, Leedu ja Moldova parlamentide kohta tõid esile seadusandliku eliidi üllatuslikult madala professionaalse taseme neis riikides: igasse parlamendikoosseisu on kerge pääseda poliitiliselt motiveeritud kuid väikese kogemuspagasiga inimestel. Lisa: Tabelid

Cabinet chooses TNK-BP, but doors remain open

Index Scriptorium Estoniae

2005-01-01

Leedu valitsus alustab Vene-Suurbritannia ühisfirmaga TNK-BP läbirääkimisi Mazeikiu Nafta aktsiaenamuse omandamiseks. Kuid samal ajal on võimalik alustada läbirääkimisi ka teiste võimalike investoritega

A comparison of the collectivist attitudes and perceptions of organisational culture among Russian-speaking organisational members in Lithuania and Estonia / Rebekka Vedina

Index Scriptorium Estoniae

Vedina, Rebekka, 1976-

2003-01-01

Uurimuse eesmärgiks oli leida seoseid kollektivistlike hoiakute ja organisatsioonikultuuri tajumise vahel vene keelt kõnelevate töötajate hulgas Leedu organisatsioonis. Saadud andmeid võrreldi Eestis varem läbi viidud uurimuse tulemustega. Tabel

Recombinant Adeno-Associated Virus-Mediated microRNA Delivery into the Postnatal Mouse Brain Reveals a Role for miR-134 in Dendritogenesis in Vivo

DEFF Research Database (Denmark)

Christensen, Mette; Larsen, Lars A; Kauppinen, Sakari

2010-01-01

delivery of microRNAs in vivo by use of recombinant adeno-associated virus (rAAV). rAAV-mediated overexpression of miR-134 in neurons of the postnatal mouse brain provided evidence for a negative role of miR-134 in dendritic arborization of cortical layer V pyramidal neurons in vivo, thereby confirming...

Tallinna Visioonikonverents 2010 : Linn ja linnakultuur 22. sajandil

Index Scriptorium Estoniae

2010-01-01

Tallinna Lauluväljaku ruumides 22. novembril toimunud Tallinna Visioonikonverentsist "Linn ja linnakultuur 22. sajandil", kus arutleti teemal, milline võiks olla Tallinn saja aasta pärast. Konverentsil esinesid Toomas Vitsut, Mart Saarma, Jan Sturesson, Raivo Raave, Satish Kumar, Juhani Pallasmaa ning moderaatoriteks olid Hardo Aasmäe ja Mati Heidmets

ELTA passes into limbo / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Leedu kirjastaja ja päevalehe Respublica omanik Vitas Tomkus ostis Elta uudistegrupi, protestiks sellele lahkusid mitmed Elta ajakirjanikud töölt. Töötajate arvates võivad omanikud hakata määrama, millistel teemadel kirjutada

Fürst Konstantin von Polock und die Geschichte Livlands im dritten Viertel des 13. Jahrhunderts / Anti Selart

Index Scriptorium Estoniae

Selart, Anti, 1973-

2006-01-01

13. ja 14. sajandi Teotooni ordu allikates esinevast vürst Konstantinist. Tema isiku identifitseerimiset. Autor peab Konstantini pärast Leedu suurvürsti Tatvila surma 1263. aastal esile kerkinud Smolenski või Polotski suguvõsa esindajaks

Polish president escapes domestic troubles during state visit / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Poola presidenti Aleksander Kwasnievskit kritiseeritakse, kuna ta ei soovi olla naftakompanii Orlen erastamisjuhtumi tunnistajaks. President sõitis visiidile Leetu, et arutada 9. mai Moskva pidustustega seonduvat ning määratleda Leedu ja Poola panust Euroopa Liidu poliitikasse

Kak estonski oboroten litovskuju korovu ukral / Jevgenia Garanzha

Index Scriptorium Estoniae

Garanža, Jevgenija, 1979-

2004-01-01

Leedu teleprogrammide ja -filmide festivalil "Amber arch" ("Merevaigust arkaad") võitis peapreemia Priit Valkna dokumentaalfilm "Hunt", mille tootis Allfilm ETV "Eesti lugude" dokumentaalsarja. Osales ka Vahur Laiapea telesari setudest "Veere pääl". Ulatuslik ülevaade festivalist

Kommertsheskaja logistika AS Paljassaare Kalatööstus / I.V Rzhannikova, T.I Savenkova

Index Scriptorium Estoniae

Rzhannikova, I.V

2005-01-01

15 aastat kalatoodete turul tegutsenud AS Paljassaare Kalatööstus (2001. aastast kuulub Leedu firma "Viciunai grupp" koosseisu) avas 2004. aastal uue logistikakeskuse. Ettevõtte juhtimisaparaat, toormega varustamise, tootmise ja müügi korraldamine. Tabelid, diagramm

Kamikaze Paksas appoints Borisov / Steven Paulikas

Index Scriptorium Estoniae

Paulikas, Steven

2004-01-01

Leedu presidendi Rolandas Paksase ümber vallandunud skandaali üks võtmeisikuid, Vene kodanik Juri Borissov teatas, et hakkab tööle presidendi ühiskondliku nõunikuna, mõni tund hiljem otsustas president distantseeruda oma valimiskampaania suursponsorist

Constitutional Courts and (Non)execution of judgments of the European Court of Human Rights: a comparison of cases from Russia and Lithuania / Ausra Padskocimaite

Index Scriptorium Estoniae

Padskocimaite, Ausra

2017-01-01

Inimõiguste kaitse konventsiooni ja põhiseaduse suhestusest ning Euroopa Inimõiguste kohtu lahendite mõjust riigisiseses õiguses Leedu ja Venemaa näitel. Vt ka artikleid samal teemal lk. 685 Itaalia ja lk. 715 Tšehhi kogemus

Sandor Liive : praegune üliodav elekter on mõne aasta pärast minevik / Sandor Liive ; interv. Raimo Poom

Index Scriptorium Estoniae

Liive, Sandor

2008-01-01

Ilmunud ka: Vesti Dnja 29. aug. lk. 6. Eesti Energia juhatuse esimees Leedu ja Soomega seotud tuumaprojektidest, elektri hinnast lähiajal, elektrituru avamisest ja CO₂ kvoodioksjonite rakendamisest 2013. aastast, CO₂ kvootide müümisest saadud tulust

TeliaSonera tahab Balti riikides enamusosalust / Annika Matson

Index Scriptorium Estoniae

Matson, Annika, 1976-

2003-01-01

Hollandi investeerimispanga ING analüüsist Eesti Telekom AS-i kohta ja TeliaSonera strateegilisest eesmärgist saada enda valdusesse enamusosalus kõigis Balti riikide telekomiettevõtetes. Diagramm: TeliaSoneral on enamusosalus hetkel vaid Leedu Telekomis

Tensions with Russia peak

Index Scriptorium Estoniae

2004-01-01

Venemaa vastas NATO radarilennukite näidislendudele Läti ja Leedu kohal omapoolsete luurelendudega Läänemere piirkonnas. Vene presidendi välisnõuniku Sergei Jastrzhembski sõnul ei kavatse Venemaa leppida NATO baaside rajamisega Balti riikide territooriumile

Estonija mozhet ostatsja bez uzkoi kolei / Jelizaveta Gavrilova

Index Scriptorium Estoniae

Gavrilova, Jelizaveta

2005-01-01

Poola-Leedu-Läti-Eesti raudteeprojekt Rail Baltica on lülitatud Euroopa Komisjoni transpordi prioriteetide hulka. Eesti probleemiks on kujunenud madal koostöövõime infrastruktuuride valduse küsimuses riigi ja erafirma Eesti Raudtee vahel

Tsiviilvastutus tuumaintsidendi põhjustatud kahju eest rahvusvahelises õiguses / Elina Saunanen

Index Scriptorium Estoniae

Saunanen, Elina

2006-01-01

Tsiviilvastutust tuumaintsidendi põhjustatud kahju eest reguleerivad peamiselt Viini konventsioon ja Pariisi konventsioon. Eesti on ratifitseerinud Viini konventsiooni, kasulik oleks ühineda ka 1997. a. täiendava hüvitamise konventsiooniga, kui seda teevad Venemaa ja Leedu

International community rallies for disaster relief

Index Scriptorium Estoniae

2005-01-01

Jaapanis toimunud ÜRO konverentsi teemadeks olid kliima soojenemine ja katastroofide ennetamine. Konverentsil Leedu delegatsiooni juhtinud siseministeeriumi ametniku sõnul asub riik suhteliselt väiksema riskiga looduskatastroofide riski piirkonnas, samas puudub Leedul vajalik tehnoloogia loodusõnnetuste prognoosimiseks

Midigraafika Linnagaleriis / Piret Pert

Index Scriptorium Estoniae

Pert, Piret

2001-01-01

Pärnu Linnagaleriis 28. juulini avatud rahvusvahelise väikegraafika näitusest "Midigrafik II". Leedu kunstniku Mikolajus Vilutise, Anu Kalmu, Helje Eelma-Saare, Sirje Eelma, Ülle Marksi ja Jüri Kassi tööde valjapanekust

Narkootilise ja psühhotroopse aine suures koguses ebaseaduslik käitlemine suure varalise kasu saamise eesmärgil : [magistritöö] / Kerli Lepik ; Tartu Ülikool, õigusteaduskond ; juhendaja: Jaan Ginter

Index Scriptorium Estoniae

Lepik, Kerli, 1989-

2013-01-01

Suures koguses narkootilise ja psühhotroopse aine käsitlemisest, suure varalise kasu saamise eesmärgist, võrdlus teiste Euroopa riikidega (EL, Läti, Leedu, Soome, Norra, Tšehhi), suurest varalisest kasust kui koosseisutunnusest ja alternatiividest

Eesti kunst Pariisis

Index Scriptorium Estoniae

2008-01-01

Eesti, läti ja leedu kaasaegse kunsti näitus Pariisis Cite Internationale des Arts galeriis. Eesti osa kuraator on Aili Vahtrapuu. Eksponeeritakse Ene-Liis Semperi, Anu Põdra, Peeter Alliku, Anu Kalmu, Virge Jõekalda jt. kunstnike loomingut

Bolshe sokov - horoshihh i raznõhh / Jevgenija Gorski

Index Scriptorium Estoniae

Gorski, Jevgenija

2006-01-01

Tänu investeeringutele kaasaegsetesse seadmetesse ja tehnoloogiatesse jätkub Põltsamaa Felixi edasine areng. Põltsamaa tehas kuulub Norras asuva toiduainetetööstuse gruppi Orkla Foods, mis ühendab Soome, Eesti, Läti ja Leedu kuut ettevõtet

NATO enlargement : all aboard? Destination unknown / Richard E Rupp

Index Scriptorium Estoniae

Rupp, Richard E

2002-01-01

NATO eesmärkidest läbi aegade, külma sõja järgse üleminekuaja mõjudest NATO arengule, laienemisega seonduvast temaatikast lähtuvalt Leedu, Rumeenia ja Venemaa seisukohtadest ning NATO tulevikuväljavaadetest

Parental reactions to the morphologically correct and incorrect utterances of children / Ingrida Balèi͠nien︠

Index Scriptorium Estoniae

Balèi͠nien︠, Ingrida

2007-01-01

Vanema reaktsioone lapse korrektsetele ja ebakorrektsetele lausungitele analüüsitakse nii kvalitatiivselt kui kvantitatiivselt, samuti uuritakse reaktsiooni sõltuvust vea tüübist. Analüüs põhineb leedu ema ja lapse vaheliste vestluste longitudinaalsel uuringul

Lower pay for equal work / Matt Garrick

Index Scriptorium Estoniae

Garrick, Matt

2011-01-01

30. juunil Vilniuses toimunud demokraatiat suurendavatele naistele pühendatud konverentsil räägiti suhtumisest naistesse ja naisjuhtidesse. Konverentsist võtsid osa ka Leedu president Dalia Grybauskaite, Soome riigipea Tarja Halonen ja USA riigisekretär Hillary Clinton

Speech by Mr. Evaldas Ignatavicius, state secretary, ministry of foreign affairs, Republic of Lithuania / Evaldas Ignatavicius

Index Scriptorium Estoniae

Ignatavicius, Evaldas

2003-01-01

Ettekanne Leedu ja Lõuna-Kaukaasia vabariikide vahelise sõjalis-poliitilise koostöö arendamisest 22.-23. septembrini 2003 Vilniuses toimunud seminaril "South Caucasus: Making the best use of external assistance for stability building and for co-operation with NATO"

Double standards criticized at democracy conference / Matt Garrick

Index Scriptorium Estoniae

Garrick, Matt

2011-01-01

30. VI -1. VII Vilniuses toimunud Demokraatiate Ühenduse kohtumisel arutati, kuidas toetada demokraatia edendamist ja ülesehitamist. Konverentsil osalesid ka USA riigisekretär Hillary Clinton ja Leedu president Dalia Grybauskaite. Järgmine ühenduse eesistujamaa on Mongoolia

Seitsmekesi majandustuultes / Erik Terk

Index Scriptorium Estoniae

Terk, Erik, 1952-

2016-01-01

Eesti majanduse seisu ja käekäigu võrdlemine teiste riigisotsialismist startinud ning ELi raames toimiva majandusega maadega. Visegrádi neliku (Tšehhi, Slovakkia, Ungari, Poola) ning Balti kolmiku (Eesti, Läti, Leedu) majanduse võrdlus

De-Russianisation of the western post-Soviet space: between the thick and thin nationalising processes / Petr Dostal ja Libor Jelen

Index Scriptorium Estoniae

Dostal, Petr

2015-01-01

Artikkel rõhutab konteksti olulisust vene mõju vähenemise protsessi käsitlemisel läänelikus postkommunistlikus ühiskonnas. Vaadeldud on Eesti, Läti, Leedu, Valgevene, Ukraina ja Moldova arenguid NSV Liidu kokkuvarisemise järgselt

Meetrika foneetikast / Ilse Lehiste

Index Scriptorium Estoniae

Lehiste, Ilse, 1922-2010

1995-01-01

Järg: nr. 9, lk. 596-603. Soome, eesti ja rootsi keele prosoodilise struktuuri ja nendes keeltes loodud luule meetrilise struktuuri omavahelistest suhetest; Leedu keele trohheilised mudelid; Rõhkude ajaline jaotus värsireas; Kokkuvõte ja järeldused

The development of logistics centres : important factor of increasing transport sector efficiency = Razvitije logistitsheskihh tsentrov : vazhnõi faktor uvelitshenija effektivnosti transportnogo sektora / Algirdas Sakalys

Index Scriptorium Estoniae

Sakalys, Algirdas

2003-01-01

Ettekanne 3.-4. detsembrini 2003 Tallinnas toimunud rahvusvahelisel konverents-näitusel "Logistics and transport in international trade" teeb ülevaate Leedu transpordisüsteemi olukorrast, arengustrateegiast ja reformidest, lähtudes Euroopa Liidu transpordipoliitika tegevuskavast. Kaardid. Diagramm. Tabel

Eestlased riisusid Balti fotovõistlusel koore

Index Scriptorium Estoniae

2011-01-01

Eesti, Läti ja Leedu fotovõistlusest, kus valiti Baltikumi parimad loodusfotod ja maaelu kajastavad fotod. Osales 259 fotograafi. Parim loodusfoto oli Kaido Haageni "Kus sa oled, mu sõber" ning parim foto maaelu kategoorias Arne Aderi "Jõuluks koju"

Normal-tissue radioprotection by overexpression of the copper-zinc and manganese superoxide dismutase genes

Energy Technology Data Exchange (ETDEWEB)

Veldwijk, Marlon R. [Dept. of Radiation Oncology, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim (Germany); Pharmacology of Cancer Treatment (G402), German Cancer Research Center, Heidelberg (Germany); Herskind, Carsten; Wenz, Frederik [Dept. of Radiation Oncology, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim (Germany); Sellner, Leopold; Zeller, W. Jens [Pharmacology of Cancer Treatment (G402), German Cancer Research Center, Heidelberg (Germany); Radujkovic, Aleksandar [Dept. of Internal Medicine V, Univ. of Heidelberg (Germany); Laufs, Stephanie [Dept. of Experimental Surgery, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim (Germany); Molecular Oncology of Solid Tumors (G360), German Cancer Research Center, Heidelberg (Germany); Fruehauf, Stefan [Center for Tumor Diagnostic and Therapy, Paracelsus-Klinik, Osnabrueck (Germany)

2009-08-15

Background and Purpose: Protection of normal tissue against radiation-induced damage may increase the therapeutic ratio of radiotherapy. A promising strategy for testing this approach is gene therapy-mediated overexpression of the copper-zinc (CuZnSOD) or manganese superoxide dismutase (MnSOD) using recombinant adeno-associated viral (rAAV2) vectors. The purpose of this study was to test the modulating effects of the SOD genes on human primary lung fibroblasts (HPLF) after irradiation. Material and Methods: HPLF were transduced with rAAV2 vectors containing cDNA for the CuZnSOD, MnSOD or a control gene. The cells were irradiated (1-6 Gy), and gene transfer efficiency, apoptosis, protein expression/activity, and radiosensitivity measured by the colony-forming assay determined. Results: After transduction, 90.0% {+-} 6.4% of the cells expressed the transgene. A significant fivefold overexpression of both SOD was confirmed by an SOD activity assay (control: 21.1 {+-} 12.6, CuZnSOD: 95.1 {+-} 17.1, MnSOD: 108.5 {+-} 36.0 U SOD/mg protein) and immunohistochemistry. CuZnSOD and MnSOD overexpression resulted in a significant radioprotection of HPLF compared to controls (surviving fraction [SF] ratio SOD/control > 1): CuZnSOD: 1.18-fold (95% confidence interval [CI]: 1.06-1.32; p = 0.005), MnSOD: 1.23-fold (95% CI: 1.07-1.43; p = 0.01). Conclusion: Overexpression of CuZnSOD and MnSOD in HPLF mediated an increase in clonogenic survival after irradiation compared to controls. In previous works, a lack of radioprotection in SOD-overexpressing tumor cells was observed. Therefore, the present results suggest that rAAV2 vectors are promising tools for the delivery of radioprotective genes in normal tissue. (orig.)

Overexpression of the steroidogenic enzyme cytochrome P450 side chain cleavage in the ventral tegmental area increases 3α,5α-THP and reduces long-term operant ethanol self-administration.

Science.gov (United States)

Cook, Jason B; Werner, David F; Maldonado-Devincci, Antoniette M; Leonard, Maggie N; Fisher, Kristen R; O'Buckley, Todd K; Porcu, Patrizia; McCown, Thomas J; Besheer, Joyce; Hodge, Clyde W; Morrow, A Leslie

2014-04-23

Neuroactive steroids are endogenous neuromodulators capable of altering neuronal activity and behavior. In rodents, systemic administration of endogenous or synthetic neuroactive steroids reduces ethanol self-administration. We hypothesized this effect arises from actions within mesolimbic brain regions that we targeted by viral gene delivery. Cytochrome P450 side chain cleavage (P450scc) converts cholesterol to pregnenolone, the rate-limiting enzymatic reaction in neurosteroidogenesis. Therefore, we constructed a recombinant adeno-associated serotype 2 viral vector (rAAV2), which drives P450scc expression and neuroactive steroid synthesis. The P450scc-expressing vector (rAAV2-P450scc) or control GFP-expressing vector (rAAV2-GFP) were injected bilaterally into the ventral tegmental area (VTA) or nucleus accumbens (NAc) of alcohol preferring (P) rats trained to self-administer ethanol. P450scc overexpression in the VTA significantly reduced ethanol self-administration by 20% over the 3 week test period. P450scc overexpression in the NAc, however, did not alter ethanol self-administration. Locomotor activity was unaltered by vector administration to either region. P450scc overexpression produced a 36% increase in (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP, allopregnanolone)-positive cells in the VTA, but did not increase 3α,5α-THP immunoreactivity in NAc. These results suggest that P450scc overexpression and the resultant increase of 3α,5α-THP-positive cells in the VTA reduces ethanol reinforcement. 3α,5α-THP is localized to neurons in the VTA, including tyrosine hydroxylase neurons, but not astrocytes. Overall, the results demonstrate that using gene delivery to modulate neuroactive steroids shows promise for examining the neuronal mechanisms of moderate ethanol drinking, which could be extended to other behavioral paradigms and neuropsychiatric pathology.

Catalytic immunoglobulin gene delivery in a mouse model of Alzheimer's disease: prophylactic and therapeutic applications.

Science.gov (United States)

Kou, Jinghong; Yang, Junling; Lim, Jeong-Eun; Pattanayak, Abhinandan; Song, Min; Planque, Stephanie; Paul, Sudhir; Fukuchi, Ken-Ichiro

2015-02-01

Accumulation of amyloid beta-peptide (Aβ) in the brain is hypothesized to be a causal event leading to dementia in Alzheimer's disease (AD). Aβ vaccination removes Aβ deposits from the brain. Aβ immunotherapy, however, may cause T cell- and/or Fc-receptor-mediated brain inflammation and relocate parenchymal Aβ deposits to blood vessels leading to cerebral hemorrhages. Because catalytic antibodies do not form stable immune complexes and Aβ fragments produced by catalytic antibodies are less likely to form aggregates, Aβ-specific catalytic antibodies may have safer therapeutic profiles than reversibly-binding anti-Aβ antibodies. Additionally, catalytic antibodies may remove Aβ more efficiently than binding antibodies because a single catalytic antibody can hydrolyze thousands of Aβ molecules. We previously isolated Aβ-specific catalytic antibody, IgVL5D3, with strong Aβ-hydrolyzing activity. Here, we evaluated the prophylactic and therapeutic efficacy of brain-targeted IgVL5D3 gene delivery via recombinant adeno-associated virus serotype 9 (rAAV9) in an AD mouse model. One single injection of rAAV9-IgVL5D3 into the right ventricle of AD model mice yielded widespread, high expression of IgVL5D3 in the unilateral hemisphere. IgVL5D3 expression was readily detectable in the contralateral hemisphere but to a much lesser extent. IgVL5D3 expression was also confirmed in the cerebrospinal fluid. Prophylactic and therapeutic injection of rAAV9-IgVL5D3 reduced Aβ load in the ipsilateral hippocampus of AD model mice. No evidence of hemorrhages, increased vascular amyloid deposits, increased proinflammatory cytokines, or infiltrating T-cells in the brains was found in the experimental animals. AAV9-mediated anti-Aβ catalytic antibody brain delivery can be prophylactic and therapeutic options for AD.

Chronic Cardiac-Targeted RNA Interference for the Treatment of Heart Failure Restores Cardiac Function and Reduces Pathological Hypertrophy

Science.gov (United States)

Suckau, Lennart; Fechner, Henry; Chemaly, Elie; Krohn, Stefanie; Hadri, Lahouaria; Kockskämper, Jens; Westermann, Dirk; Bisping, Egbert; Ly, Hung; Wang, Xiaomin; Kawase, Yoshiaki; Chen, Jiqiu; Liang, Lifan; Sipo, Isaac; Vetter, Roland; Weger, Stefan; Kurreck, Jens; Erdmann, Volker; Tschope, Carsten; Pieske, Burkert; Lebeche, Djamel; Schultheiss, Heinz-Peter; Hajjar, Roger J.; Poller, Wolfgang Ch.

2009-01-01

Background RNA interference (RNAi) has the potential to be a novel therapeutic strategy in diverse areas of medicine. We report on targeted RNAi for the treatment of heart failure (HF), an important disorder in humans resulting from multiple etiologies. Successful treatment of HF is demonstrated in a rat model of transaortic banding by RNAi targeting of phospholamban (PLB), a key regulator of cardiac Ca2+ homeostasis. Whereas gene therapy rests on recombinant protein expression as its basic principle, RNAi therapy employs regulatory RNAs to achieve its effect. Methods and Results We describe structural requirements to obtain high RNAi activity from adenoviral (AdV) and adeno-associated virus (AAV9) vectors and show that an AdV short hairpin RNA vector (AdV-shRNA) silenced PLB in cardiomyocytes (NRCMs) and improved hemodynamics in HF rats 1 month after aortic root injection. For simplified long-term therapy we developed a dimeric cardiotropic AAV vector (rAAV9-shPLB) delivering RNAi activity to the heart via intravenous injection. Cardiac PLB protein was reduced to 25% and SERCA2a suppression in the HF groups was rescued. In contrast to traditional vectors rAAV9 shows high affinity for myocardium, but low affinity for liver and other organs. rAAV9-shPLB therapy restored diastolic (LVEDP, dp/dtmin, Tau) and systolic (fractional shortening) functional parameters to normal range. The massive cardiac dilation was normalized and the cardiac hypertrophy, cardiomyocyte diameter and cardiac fibrosis significantly reduced. Importantly, there was no evidence of microRNA deregulation or hepatotoxicity during these RNAi therapies. Conclusion Our data show, for the first time, high efficacy of an RNAi therapeutic strategy in a cardiac disease. PMID:19237664

Overexpression of suppressor of cytokine signaling 3 in the arcuate nucleus of juvenile Phodopus sungorus alters seasonal body weight changes.

Science.gov (United States)

Ganjam, Goutham K; Benzler, Jonas; Pinkenburg, Olaf; Boucsein, Alisa; Stöhr, Sigrid; Steger, Juliane; Culmsee, Carsten; Barrett, Perry; Tups, Alexander

2013-12-01

The profound seasonal cycle in body weight exhibited by the Djungarian hamster (Phodopus sungorus) is associated with the development of hypothalamic leptin resistance during long day photoperiod (LD, 16:8 h light dark cycle), when body weight is elevated relative to short day photoperiod (SD, 8:16 h light dark cycle). We previously have shown that this seasonal change in physiology is associated with higher levels of mRNA for the potent inhibitor of leptin signaling, suppressor of cytokine signaling-3 (SOCS3), in the arcuate nucleus (ARC) of LD hamsters relative to hamsters in SD. The alteration in SOCS3 gene expression preceded the body weight change suggesting that SOCS3 might be the molecular switch of seasonal body weight changes. To functionally characterize the role of SOCS3 in seasonal body weight regulation, we injected SOCS3 expressing recombinant adeno-associated virus type-2 (rAAV2-SOCS3) constructs into the ARC of leptin sensitive SD hamsters immediately after weaning. Hamsters that received rAAV2 expressing enhanced green fluorescent protein (rAAV2-EGFP) served as controls. ARC-directed SOCS3 overexpression led to a significant increase in body weight over a period of 12 weeks without fully restoring the LD phenotype. This increase was partially due to elevated brown and white adipose tissue mass. Gene expression of pro-opiomelanocortin was increased while thyroid hormone converting enzyme DIO3 mRNA levels were reduced in SD hamsters with SOCS3 overexpression. In conclusion, our data suggest that ARC-directed SOCS3 overexpression partially overcomes the profound seasonal body weight cycle exhibited by the hamster which is associated with altered pro-opiomelanocortin and DIO3 gene expression.

Phase 2 clinical trial of a recombinant adeno-associated viral vector expressing α1-antitrypsin: interim results.

LENUS (Irish Health Repository)

Flotte, Terence R

2011-10-01

Recombinant adeno-associated virus (rAAV) vectors offer promise for the gene therapy of α(1)-antitrypsin (AAT) deficiency. In our prior trial, an rAAV vector expressing human AAT (rAAV1-CB-hAAT) provided sustained, vector-derived AAT expression for >1 year. In the current phase 2 clinical trial, this same vector, produced by a herpes simplex virus complementation method, was administered to nine AAT-deficient individuals by intramuscular injection at doses of 6.0×10(11), 1.9×10(12), and 6.0×10(12) vector genomes\\/kg (n=3 subjects\\/dose). Vector-derived expression of normal (M-type) AAT in serum was dose dependent, peaked on day 30, and persisted for at least 90 days. Vector administration was well tolerated, with only mild injection site reactions and no serious adverse events. Serum creatine kinase was transiently elevated on day 30 in five of six subjects in the two higher dose groups and normalized by day 45. As expected, all subjects developed anti-AAV antibodies and interferon-γ enzyme-linked immunospot responses to AAV peptides, and no subjects developed antibodies to AAT. One subject in the mid-dose group developed T cell responses to a single AAT peptide unassociated with any clinical effects. Muscle biopsies obtained on day 90 showed strong immunostaining for AAT and moderate to marked inflammatory cell infiltrates composed primarily of CD3-reactive T lymphocytes that were primarily of the CD8(+) subtype. These results support the feasibility and safety of AAV gene therapy for AAT deficiency, and indicate that serum levels of vector-derived normal human AAT >20 μg\\/ml can be achieved. However, further improvements in the design or delivery of rAAV-AAT vectors will be required to achieve therapeutic target serum AAT concentrations.

The effect of surface demineralization of cortical bone allograft on the properties of recombinant adeno-associated virus coatings.

Science.gov (United States)

Yazici, Cemal; Yanoso, Laura; Xie, Chao; Reynolds, David G; Samulski, R Jude; Samulski, Jade; Yannariello-Brown, Judith; Gertzman, Arthur A; Zhang, Xinping; Awad, Hani A; Schwarz, Edward M

2008-10-01

Freeze-dried recombinant adeno-associated virus (rAAV) coated structural allografts have emerged as an approach to engender necrotic cortical bone with host factors that will persist for weeks following surgery to facilitate revascularization, osteointegration, and remodeling. However, one major limitation is the nonporous cortical surface that prohibits uniform distribution of the rAAV coating prior to freeze-drying. To overcome this we have developed a demineralization method to increase surface absorbance while retaining the structural integrity of the allograft. Demineralized bone wafers (DBW) made from human femoral allograft rings demonstrated a significant 21.1% (73.6+/-3.9% versus 52.5+/-2.6%; pcoating versus mineralized controls. Co-incubation of rAAV-luciferase (rAAV-Luc) coated DBW with a monolayer of C3H10T1/2 cells in culture led to peak luciferase levels that were not significantly different from soluble rAAV-Luc controls (p>0.05), although the peaks occurred at 60h and 12h, respectively. To assess the transduction efficiency of rAAV-Luc coated DBW in vivo, we first performed a dose response with allografts containing 10(7), 10(9) or 10(10) particles that were surgically implanted into the quadriceps of mice, and assayed by in vivo bioluminescence imaging (BLI) on days 1, 3, 5, 7, 10, 14, and 21. The results demonstrated a dose response in which the DBW coated with 10(10) rAAV-Luc particles achieved peak gene expression levels on day 3, which persisted until day 21, and was significantly greater than the 10(7) dose throughout this time period (pcoated with 10(10) rAAV-Luc particles failed to demonstrate any significant differences in transduction kinetics or efficiency in vivo. Thus, surface demineralization of human cortical bone allograft increases its absorbance for uniform rAAV coating, without affecting vector transduction efficiency.

Kunstitudengid näitavad Maarjamäe lossi pargis oma vabadussamba tõlgendusi

Index Scriptorium Estoniae

2008-01-01

Eesti Kunstiakadeemia skulptuuritudengid Marit Mardla, Matthias-Jakob Sildnik, Mikk Madisson, Liis Dvorjanski, Uku Sepisvart, Hannes Aasamets, Kadri Allekand, Kristin Orav, Loore Raav, Jekaterina Kultajeva, Li Yang, Kristel Aasjõe, Liivi Tantaal, Jass Kaselaan, Edith Karlson, Kadri Metstak, Berit Talpsepp ja Eike Eplik näitavad oma töid näitusel "Vabaduse monumendid"

Problems of implementation the total quality management in the Lithuanian road building enterprises / Julius Christauskas

Index Scriptorium Estoniae

Christauskas, Julius

2005-01-01

Leedu tee-ehitusettevõtete juhtimine ei ole suunatud protsessi kui terviku kontrollile ja tulemuslikule meeskonnatööle, töötajad ei ole valmis võtma suuremat vastutust, puudu jääb efektiivsusest, loovusest ja innovatiivsusest. Tabelid. Graafikud

Passing the budget / Rokas M. Tracevskis

Index Scriptorium Estoniae

Tracevskis, Rokas M.

2009-01-01

7. detsembril kohtus Leedu president Dalia Grybauskaite parlamendifraktsioonide esimeestega, paludes neid 10. detsembril 2010. aasta riigieelarve vastu võtta. 10. detsembril on oodata ka meeleavaldust. Rahandusminister Ingrida Simonyte prognoosi järgi moodustab 2010. aastal riigivõlg 41% SKP-st

Leedus pandi homoinfo keelu alla / Kaivo Kopli

Index Scriptorium Estoniae

Kopli, Kaivo

2009-01-01

Leedu parlament kiitis teist korda heaks seaduse "Alaealiste kaitsmisest negatiivse informatsiooni eest" (Law on the Protection of Minors against the Detrimental Effect of Public Information ; Nepilnameciu apsaugos nuo neigiamo viešosios informacijos poveikio). Lisa: Grybauskaite näeb homofoobiat

Labor's charm wins over countryside / Milda Seputyte

Index Scriptorium Estoniae

Seputyte, Milda

2005-01-01

Küsitluste järgi on Leedu Tööpartei toetus elanike seas tõusnud 25,3 protsendini. Juhul kui praegune peaminister Algirdas Brazauskas peaks skandaali tõttu ametist tagasi astuma, kavatseb sellele kohale kandideerida Tööpartei esimees Viktor Uspaskihh

Лаборатория безумной моды / Светлана Карабут

Index Scriptorium Estoniae

Карабут, Светлана

2009-01-01

30. mail Eesti Kunstiakadeemia aulas ja siseõues toimunud ERKI moeshow'l näidati 26 kollektsiooni eesti tudengitelt ning Leedu, Ungari ja Gruusia külalistelt. Külalised olid rahvusvahelise noorte disainerite konkursi Create Europe Fashion Academy Award auhinnasaajad

LNG terminal võib tulla ka igasse Balti riiki. Arutlused esialgu jätkuvad / Sirje Rank

Index Scriptorium Estoniae

Rank, Sirje, 1966-

2011-01-01

Balti peaministrid pole üksmeelel, kas ja kuhu rajada veeldatud gaasi terminal. Lepiti kokku, et kõik kolm riiki teevad vedelgaasi terminali tasuvusuuringu. Leedu kavatseb rajada oma riigi vajadusteks LNG ujuvterminali, kuid see ei välista osalemist suurema terminali projektis

Recycling efforts behind schedule / Matt Garrick

Index Scriptorium Estoniae

Garrick, Matt

2011-01-01

Vastavalt Euroopa Liidu direktiivile peab Leedu aastaks 2013 saama taaskasutada või nõuetekohaselt käidelda 50% jäätmetest. 2011. aasta esimeses pooles oli nõuetekohaselt käideldud vaid 5% jäätmetest. Riigis plaanitavatest projektidest

Sistemnaja bezopasnost / Josef Kats

Index Scriptorium Estoniae

Kats, Josef, 1977-

2007-01-01

Euroopa Parlamendi kodanike õiguste, justiits- ja siseasjade komisjon leidis, et Eesti, Läti, Leedu, Poola, Slovakkia, Sloveenia, Tšehhi, Ungari ja Malta on täitnud Schengeni infosüsteemi SIS rakendamiseks vajalikud tingimused ja esitas oma hinnangu nõukogule

Helping hand from the Baltics: Lithuania deepens support for Georgia / Michael Durnan

Index Scriptorium Estoniae

Durnan, Michael

2009-01-01

Augustisõjast räsitud Gruusia pealinnas toimunud massimeeleavaldustel nõuti president Mihhail Saakašvili tagasiastumist. Leedu president Valdas Adamkus kutsub lääneriike avaldama suuremat toetust Gruusiale ning mõistab hukka Vene Föderatsiooni Gruusia-vastase tegevuse

Russia must face January 13 past - PM / Justinas Vainilavicius

Index Scriptorium Estoniae

Vainilavicius, Justinas

2009-01-01

Leedu peaminister Andrius Kubilius teatas, et Venemaa peab võtma vastutuse 13. jaanuaril 1991. aastal aset leidnud sündmuste ja 14 inimese hukkumise eest. Euroopa Komisjon soovib, et Venemaa teeks koostööd nende sündmuste uurimisel