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Sample records for publications faculties mcl

  1. Faculty Research and Publication Practices

    Science.gov (United States)

    Zoellner, Kate; Hines, Samantha; Keenan, Teressa; Samson, Sue

    2015-01-01

    Understanding faculty work practices can translate into improved library services. This study documents how education and behavioral science faculty locate, retrieve, and use information resources for research and writing and how they publish and store their research materials. The authors interviewed twelve professors using a structured interview…

  2. Writing for publication: faculty development initiative using social learning theory.

    Science.gov (United States)

    Sanderson, Bonnie K; Carter, Matt; Schuessler, Jenny B

    2012-01-01

    Demonstrating scholarly competency is an expectation for nurse faculty. However, there is hesitancy among some faculty to fully engage in scholarly activities. To strengthen a school of nursing's culture of scholarship, a faculty development writing initiative based on Social Learning Theory was implemented. The authors discuss this initiative to facilitate writing for publication productivity among faculty and the successful outcomes.

  3. SHOULD WE HAVE FACULTIES OF PUBLIC HEALTH?

    Science.gov (United States)

    Hill, H W

    1924-02-15

    Public health is the science and art of conscious physical adjustment between man and his surroundings in the universe. The modern conception of man as a product of and a part of nature brings the subject of man's individual physical adjustments with his immediate surroundings into its proper place as the fundamental study-the basis of every form of education. Hence, public health is not only eligible for a position as an independent faculty in any university but is as definitely entitled to such a place as any of those now recognized. It is futile to consider the ordinary 45 hour course in public health, furnished as an incident in the ordinary 4000 to 5000 hour medical course, as more than a smattering, offered to medical students alone, of the 900 to 4500 hour courses in public health offered to professional public health students.

  4. The "Big Bang" in Public and Private Faculty Salaries

    Science.gov (United States)

    Rippner, Jennifer A.; Toutkoushian, Robert K.

    2015-01-01

    The gap between average faculty salaries at public and private institutions has been growing wider over the past 40 years, yet little is known about the nature and causes of the gap. This study uses data on more than 1,000 institutions to examine institutional average faculty salaries and how they have changed for public and private institutions.…

  5. How To Achieve Faculty Diversity In Public Higher Education: Minority Faculty Preferences And Their Alternatives

    Directory of Open Access Journals (Sweden)

    Ronald L. Taylor

    2011-07-01

    Full Text Available Most educators in American public higher education vigorously embrace the goal of diversity for both faculty and students.  One approach frequently utilized is preferential programs for targeted minorities for both faculty employment and student admissions.  The Supreme Court has recently recognized that achieving a diverse student body in a public college or university is a sufficiently important interest to constitutionally justify implementation of a preferential admissions plan for minority students.  The unanswered issue explored in this article is whether diversity may similarly serve as a sufficient justification for preferential hiring of minority faculty candidates.  Also discussed are alternatives that institutions of higher learning may pursue in order to achieve faculty diversity without utilizing race- or ethnic-conscious faculty preferential hiring programs.

  6. Bowie State College Teaching Faculty's Attitudes toward Public Education.

    Science.gov (United States)

    Gill, Wanda E.; And Others

    This survey sought the opinions of faculty members at Bowie State College on 40 questions concerning academic and societal issues in public education. Responses to the survey questionnaire by faculty members represented a return of 14.5 percent, or 15 individuals. Tables are included comparing the responses of the Bowie State College sample and…

  7. Factors and Strategies That Influence Faculty Involvement in Public Service

    Science.gov (United States)

    Holland, Barbara A.

    2016-01-01

    Data from several national research and evaluation projects involving 32 diverse institutions offer insights into what motivates college-faculty involvement in public service and outreach efforts, what faculty find to be obstacles to involvement, and what institutions can do to facilitate involvement. One salient policy supporting faculty…

  8. Publication Productivity of Faculty of Medicine, Mansoura University ...

    African Journals Online (AJOL)

    Medicine comprises 32 departments either clinical or preclinical.[16]. To the best ... PubMed-indexed medical publications from Mansoura Faculty ... 'Case reports', 'Cohort study', 'Descriptive', 'Experimental', ..... reproductive medicine: Egypt.

  9. Supporting faculty proposal development and publication.

    Science.gov (United States)

    Anders, Robert L; Monsivais, Diane

    2006-01-01

    The Hispanic Health Disparities Research Center, a collaborative venture between the University of Texas at El Paso and the University of Texas at Houston Health Sciences Center, supports the research capabilities of junior faculty through a variety of programs. Novice researchers often need practical help in conducting literature reviews, extracting data, evaluating the evidence, and formulating a research question of significant importance to be funded yet narrow enough to fit within the scope of the proposal. The authors discuss a successful proposal development program that includes mentoring by more senior faculty and structured sessions with a medical writer and editor.

  10. It's academic: public policy activities among faculty members in a department of medicine.

    Science.gov (United States)

    Jacobs, Douglas B; Greene, Meredith; Bindman, Andrew B

    2013-10-01

    To investigate whether and how faculty members in a department of medicine are engaged in public policy activities. Between February and April 2011, the authors conducted a cross-sectional, Web-based survey of all active Department of Medicine faculty members at the University of California, San Francisco. Survey questions covered demographics, academic role, academic rank, and participation in three specific public policy activities during the past five years: (1) policy-related research, (2) expert advice to government officials, and (3) public policy advocacy in collaboration with organizations outside government. Two hundred twenty of 553 faculty (40%) responded to the survey. One hundred twenty-four faculty members (56% of respondents and 22% of total active faculty) reported that they were engaged in at least one of the three types of policy-related activities: 51 (23%) conducted policy-related research, 67 (30%) provided expert advice to government officials, and 93 (42%) collaborated with organizations to advocate for public policy. Higher faculty rank was significantly associated with faculty members reporting that they were involved in one or more of the three policy activities (P = .04). Academic departments should identify public policy expertise among their faculty and leverage this expertise by facilitating opportunities to develop a shared faculty awareness of their public policy activities, by supporting the establishment of mentoring relationships for less experienced faculty in the area of public policy, and by incorporating standards of excellence for work in public policy into the promotions process.

  11. Pharmaceutical science faculty publication records at research-intensive pharmacy colleges and schools.

    Science.gov (United States)

    Thompson, Dennis F; Nahata, Milap C

    2012-11-12

    To determine yearly (phase 1) and cumulative (phase 2) publication records of pharmaceutical science faculty members at research-intensive colleges and schools of pharmacy. The publication records of pharmaceutical science faculty members at research-intensive colleges and schools of pharmacy were searched on Web of Science. Fifty colleges and schools of pharmacy were randomly chosen for a search of 1,042 individual faculty members' publications per year from 2005 to 2009. A stratified random sample of 120 faculty members also was chosen, and cumulative publication counts were recorded and bibliometric indices calculated. The median number of publications per year was 2 (range, 0-34). Overall, 22% of faculty members had no publications in any given year, but the number was highly variable depending on the faculty members' colleges or schools of pharmacy. Bibliometric indices were higher for medicinal chemistry and pharmaceutics, with pharmacology ranking third and social and administrative sciences fourth. Higher bibliometric indices were also observed for institution status (ie, public vs private) and academic rank (discipline chairperson vs non-chairperson and professor vs junior faculty member) (pscience disciplines and academic ranks within research-intensive colleges and schools of pharmacy. These data may be important for benchmarking purposes.

  12. Faculty Voice in Intellectual Property Policies: Collective Action for the Public Good

    Science.gov (United States)

    Kezar, Adrianna

    2017-01-01

    This chapter synthesizes the previous chapters to offer to readers practical recommendations for navigating IP issues, including resources and advice for faculty and administrations to engage in more equitable discussions about the rights of faculty and the interests of the public good.

  13. Publication Patterns of Male and Female Faculty Members in the Communication Discipline.

    Science.gov (United States)

    Nadler, Lawrence B.; Nadler, Marjorie Keeshan

    2001-01-01

    Examines sex-based patterns regarding research productivity. Examines the literature regarding sex-based publication patterns; delineates research questions concerning these key variables; reports results of a study regarding publication patterns; and addresses implications of the study's findings for faculty and administrators. Finds that male…

  14. Journal publications by pharmacy practice faculty evaluated by institution and region of the United States (2001-2003

    Directory of Open Access Journals (Sweden)

    Coleman CI

    2007-12-01

    Full Text Available Objective: To compare the quantity of manuscripts published in journals by departments of pharmacy practice at schools and colleges of pharmacy in the United States for the years 2001-2003.Methods: We utilized the Web of Science bibliographic database to identify publication citations for the years 2001 to 2003 which were then evaluated in a number of different ways. Faculty were identified via American Association of Colleges of Pharmacy rosters for 2000-2001, 2001-2002, and 2002-2003 academic years. Results: Rankings were done based on the number of publications per institution and average number of publications per faculty member at an institution. Upon linear regression analysis, a relationship exists between an institution’s faculty size and the total number of publications but not for tenure/nontenure-track faculty ratio. Rating highest in overall publication number did not guarantee high rankings in the average number of publications per faculty member at an institution assessment. Midwestern schools were responsible for more publications per institution than other regions. Many schools only generated minimal scholarship over this evaluative period.Conclusion: While many schools have pharmacy practice faculty that strongly contributed to the biomedical literature, other schools have not. Pharmacy practice faculty in the Midwest publish more journal manuscripts than faculty in other regions of the country. More pharmacy schools need to engage their faculty in scholarly endeavors by providing support and incentives.

  15. Nordic MCL2 trial update

    DEFF Research Database (Denmark)

    Geisler, Christian H; Kolstad, Arne; Laurell, Anna

    2012-01-01

    Mantle cell lymphoma (MCL) is a heterogenic non-Hodgkin lymphoma entity, with a median survival of about 5 years. In 2008 we reported the early - based on the median observation time of 4 years - results of the Nordic Lymphoma Group MCL2 study of frontline intensive induction immunochemotherapy...... and autologous stem cell transplantation (ASCT), with more than 60% event-free survival at 5 years, and no subsequent relapses reported. Here we present an update after a median observation time of 6·5 years. The overall results are still excellent, with median overall survival and response duration longer than...

  16. Organisational Culture: An Exploratory Study Comparing Faculties' Perspectives within Public and Private Universities in Malaysia

    Science.gov (United States)

    Ramachandran, Sharimllah Devi; Chong, Siong Choy; Ismail, Hishamuddin

    2011-01-01

    Purpose: The main objective of this paper to study the organisational culture (OC) in private and public higher education institutions (HEIs) from the perspective of faculty members in order to provide empirical insights on the differences and consequently pave an avenue for cross-learning. Design/methodology/approach: Data were collected from 594…

  17. Efficiency at Faculties of Economics in the Czech Public Higher Education Institutions: Two Different Approaches

    Science.gov (United States)

    Flégl, Martin; Vltavská, Kristýna

    2013-01-01

    The paper evaluates research and teaching efficiency at faculties of economics in the public higher education institutions in the Czech Republic. Evaluation is provided in two periods between the years 2006-2010 and 2007-2011. For this evaluation the Data Envelopment Analysis and Index approach are used. Data Envelopment Analysis measures research…

  18. Organisational Culture: An Exploratory Study Comparing Faculties' Perspectives within Public and Private Universities in Malaysia

    Science.gov (United States)

    Ramachandran, Sharimllah Devi; Chong, Siong Choy; Ismail, Hishamuddin

    2011-01-01

    Purpose: The main objective of this paper to study the organisational culture (OC) in private and public higher education institutions (HEIs) from the perspective of faculty members in order to provide empirical insights on the differences and consequently pave an avenue for cross-learning. Design/methodology/approach: Data were collected from 594…

  19. PRACTITIONER AND FACULTY PERSPECTIVES ON THE CAREER PREPARATION OF ENTRY-LEVEL PUBLIC ACCOUNTANTS

    Directory of Open Access Journals (Sweden)

    Kathryn Simms

    2012-01-01

    Full Text Available Entry-level public accountants in the U.S. must satisfy a litany of historically unparalleled requirements and demands. This unique set of challenges calls forth new questions about how effectively entry-level accountants are navigating the divide between their conceptual educations and the practical rigors of public practice. To examine these questions, we relied on qualitative analysis predicated on a theoretical framework of constructivism and systems theory. Our study confirmed practitioners’ perceptions about the preparation of entry-level accountants that have been documented for nearly a hundred years: Entry-level accountants’ shortcomings often include written and oral communications skills, interpersonal skills and critical thinking skills. However, what is unique to this study is that we also considered faculty perspectives. Faculty concurred with practitioners’ perspectives on entry-level accountants’ strengths and weaknesses-noting considerable growth in most problem areas over the college years. Practitioners and faculty also largely agreed about the pathway to successful and unsuccessful careers in public accounting. We suggest that continuing the historical perspective of extreme separation between academia and the business world is not particularly beneficial to the career preparation of junior accountants. Rather, we recommend that viewing accounting faculty and practitioners as part of the same continuum is likely to be more advantageous to the preparation of entry-level accountants and to the profession as a whole. We also conclude that differences in faculty and practitioner perspectives serve as checks and balances on the accounting profession-although more collaboration might facilitate greater improvements.

  20. Public Outreach for the International Year of Astronomy Through Faculty and Science Center Partnerships

    Science.gov (United States)

    Zentner, Andrew

    2009-03-01

    The International Year of Astronomy 2009 provides an opportunity to jump-start public education and outreach programs and to engage the community in a fascinating field. In my talk I will discuss a diverse program of education and outreach designed and implemented as a collaborative effort between the Astronomy faculty at the University of Pittsburgh and the Carnegie Science Center. I will highlight some of the unique benefits of such a partnership and some of the unique events such a partnership enables.

  1. Balancing Institutional, Disciplinary and Faculty Priorities with Public and Social Needs: Defining Scholarship for the 21St Century

    Science.gov (United States)

    Diamond, Robert M.; Adam, Bronwyn E.

    2004-01-01

    By virtue of their education, training, and experience, college and university faculty are a rich national resource. Opportunities abound for faculty scholars to apply their knowledge and expertise to pressing community and public issues and problems. Yet, narrow definitions of scholarship embedded in higher education practice act as disincentives…

  2. The gender gap in peer-reviewed publications by physical therapy faculty members: a productivity puzzle.

    Science.gov (United States)

    Kaufman, Regina R; Chevan, Julia

    2011-01-01

    Studies of peer-reviewed article publication by faculty in higher education show men publish more than women. Part of the difference in publishing appears to be attributable directly to gender. Gender differences in publishing productivity have not been explored in physical therapy. The purpose of this study was to explore effects of gender on peer-reviewed publication productivity in physical therapy. This was a cross-sectional study using survey methods. A survey was administered to a random sample of 881 physical therapy faculty members; 459 responses were used for analysis. Men were more likely than women to be married, have children, hold a PhD degree, be tenured or on a tenure track, and hold the position of department chair. There was a significant difference in peer-reviewed publication rates between male and female respondents. Negative binomial regression models revealed that female gender was a negative predictor of peer-reviewed publication, accounting for between 0.51 and 0.58 fewer articles per year for women than for men over the course of a career. Reasons for the gender differences are not clear. Factors such as grant funding, laboratory resources, nature of collaborative relationships, values for different elements of the teaching/research/service triad, and ability to negotiate the academic culture were not captured by our model. The gender gap in peer-reviewed publishing productivity may have implications for individuals and the profession of physical therapy and should be subject to further exploration.

  3. Open access of publications by veterinary faculty in the United States and Canada.

    Science.gov (United States)

    Nault, André J

    2011-01-01

    The free availability of full-text veterinary publications in MEDLINE-indexed journals by US and Canadian veterinary faculty from 2006-7 was determined. Additionally, publishing databases were searched to obtain general statistics on veterinary publishing. A survey of institutional initiatives to promote open-access journals and institutional repositories was also performed. Veterinary faculty published a total of 4,872 articles indexed by MEDLINE in 679 different journals. Of these articles, 1,334 (27%) were available as free full text and were published in 245 different journals. Although 51 veterinary-specific journals offering immediate and free full-text access were identified, few articles in this study appeared in these titles. Rather, most free scholarly articles by veterinary faculty appeared in journals with an embargo period. Academic veterinary institutions may want to recommend acceptance of alternate forms of information dissemination (such as open-access journals and journals published only digitally) to encourage greater global dissemination of their research findings. The promotion and use of digital institutional repositories is also an area for future investment and warrants additional research.

  4. Citation analysis of faculty publication: beyond Science Citation Index and Social Science Citation Index.

    Science.gov (United States)

    Reed, K L

    1995-10-01

    When evaluated for promotion or tenure, faculty members are increasingly judged more on the quality than on the quantity of their scholarly publications. As a result, they want help from librarians in locating all citations to their published works for documentation in their curriculum vitae. Citation analysis using Science Citation Index and Social Science Citation Index provides a logical starting point in measuring quality, but the limitations of these sources leave a void in coverage of citations to an author's work. This article discusses alternative and additional methods of locating citations to published works.

  5. The Public Good and Academic Capitalism: Science and Engineering Doctoral Students and Faculty on the Boundary of Knowledge Regimes

    Science.gov (United States)

    Szelényi, Katalin; Bresonis, Kate

    2014-01-01

    This article examines the research-related experiences of 48 doctoral students and 22 faculty in science and engineering fields at three research universities, with specific emphasis on the intersection of the public good and academic capitalism. Identifying an expansive, intersecting organizational space between the public good and academic…

  6. The Public Good and Academic Capitalism: Science and Engineering Doctoral Students and Faculty on the Boundary of Knowledge Regimes

    Science.gov (United States)

    Szelényi, Katalin; Bresonis, Kate

    2014-01-01

    This article examines the research-related experiences of 48 doctoral students and 22 faculty in science and engineering fields at three research universities, with specific emphasis on the intersection of the public good and academic capitalism. Identifying an expansive, intersecting organizational space between the public good and academic…

  7. May a Public University Restrict Faculty Expression on Its Internet World Wide Web Sites? Academic Freedom and University Facility Use Restrictions.

    Science.gov (United States)

    Allred, Lisa R.

    1997-01-01

    Public university restriction of faculty expression on the institution's World Wide Web server is discussed based on recent Supreme Court decisions. It is proposed that in some circumstances, content-based restriction of faculty expression is permissible and will not violate the First Amendment academic freedom rights of faculty. (MSE)

  8. Awareness of Accessibility Barriers in Computer-Based Instructional Materials and Faculty Demographics at South Dakota Public Universities

    Science.gov (United States)

    Olson, Christopher

    2013-01-01

    Advances in technology and course delivery methods have enabled persons with disabilities to enroll in higher education at an increasing rate. Federal regulations state persons with disabilities must be granted equal access to the information contained in computer-based instructional materials, but faculty at the six public universities in South…

  9. An Organizational Culture Study of Missouri State University Faculty/Staff in Relation to the University's Public Affair Mission

    Science.gov (United States)

    Weaver, Marissa LeClaire

    2012-01-01

    The purpose of the study was to address a problem of practice of the public affairs mission through the perceptions of faculty and staff members at Missouri State University of the University's organizational culture. The design included a phenomenological study with a set of organizational culture procedural questions related to the perceptions…

  10. University Restructuring and the Reconfiguration of Faculty Members' Work Context in a Public State University in Mexico

    Science.gov (United States)

    Montero-Hernandez, Virginia; Levin, John S.

    2013-01-01

    This study describes the effects of neo-liberal restructuring for universities upon the reconfiguration of academics' work context in a public state university in Mexico. Findings show that implementation of the federal program titled Faculty Enhancement Program during the late 1990s created a separation between traditional and new academic…

  11. Virtual screening for potential inhibitors of Mcl-1 conformations sampled by normal modes, molecular dynamics, and nuclear magnetic resonance

    Directory of Open Access Journals (Sweden)

    Glantz-Gashai Y

    2017-06-01

    Full Text Available Yitav Glantz-Gashai,* Tomer Meirson,* Eli Reuveni, Abraham O Samson Faculty of Medicine in the Galilee, Bar Ilan University, Safed, Israel *These authors contributed equally to this work Abstract: Myeloid cell leukemia-1 (Mcl-1 is often overexpressed in human cancer and is an important target for developing antineoplastic drugs. In this study, a data set containing 2.3 million lead-like molecules and a data set of all the US Food and Drug Administration (FDA-approved drugs are virtually screened for potential Mcl-1 ligands using Protein Data Bank (PDB ID 2MHS. The potential Mcl-1 ligands are evaluated and computationally docked on to three conformation ensembles generated by normal mode analysis (NMA, molecular dynamics (MD, and nuclear magnetic resonance (NMR, respectively. The evaluated potential Mcl-1 ligands are then compared with their clinical use. Remarkably, half of the top 30 potential drugs are used clinically to treat cancer, thus partially validating our virtual screen. The partial validation also favors the idea that the other half of the top 30 potential drugs could be used in the treatment of cancer. The normal mode-, MD-, and NMR-based conformation greatly expand the conformational sampling used herein for in silico identification of potential Mcl-1 inhibitors. Keywords: virtual screening, Mcl-1, molecular dynamics, NMR, normal modes

  12. The MCL-1 BH3 helix is an exclusive MCL-1 inhibitor and apoptosis sensitizer

    Energy Technology Data Exchange (ETDEWEB)

    Stewart, Michelle L.; Fire, Emiko; Keating, Amy E.; Walensky, Loren D. (MIT); (DFCI)

    2010-08-30

    The development of selective inhibitors for discrete anti-apoptotic BCL-2 family proteins implicated in pathologic cell survival remains a formidable but pressing challenge. Such precisely tailored compounds would serve as molecular probes and targeted therapies to study and treat human diseases driven by specific anti-apoptotic blockades. In particular, MCL-1 has emerged as a major resistance factor in human cancer. By screening a library of stabilized alpha-helix of BCL-2 domains (SAHBs), we determined that the MCL-1 BH3 helix is itself a potent and exclusive MCL-1 inhibitor. X-ray crystallography and mutagenesis studies defined key binding and specificity determinants, including the capacity to harness the hydrocarbon staple to optimize affinity while preserving selectivity. MCL-1 SAHB directly targets MCL-1, neutralizes its inhibitory interaction with pro-apoptotic BAK and sensitizes cancer cells to caspase-dependent apoptosis. By leveraging nature's solution to ligand selectivity, we generated an MCL-1-specific agent that defines the structural and functional features of targeted MCL-1 inhibition.

  13. Including the Majority: Academic and Social Inclusion of Adjunct Faculty at Selected Texas Public Community Colleges

    Science.gov (United States)

    Spaniel, Suzann Holland

    2012-01-01

    As the majority of teaching faculty on many community college campuses, adjuncts are accountable for the higher education of an increasing number of college-going students. However, adjunct faculty often are disconnected from the community colleges that depend upon them. The purpose of this nonexperimental quantitative study was to investigate the…

  14. Coverage and quality: A comparison of Web of Science and Scopus databases for reporting faculty nursing publication metrics.

    Science.gov (United States)

    Powell, Kimberly R; Peterson, Shenita R

    2017-03-11

    Web of Science and Scopus are the leading databases of scholarly impact. Recent studies outside the field of nursing report differences in journal coverage and quality. A comparative analysis of nursing publications reported impact. Journal coverage by each database for the field of nursing was compared. Additionally, publications by 2014 nursing faculty were collected in both databases and compared for overall coverage and reported quality, as modeled by Scimajo Journal Rank, peer review status, and MEDLINE inclusion. Individual author impact, modeled by the h-index, was calculated by each database for comparison. Scopus offered significantly higher journal coverage. For 2014 faculty publications, 100% of journals were found in Scopus, Web of Science offered 82%. No significant difference was found in the quality of reported journals. Author h-index was found to be higher in Scopus. When reporting faculty publications and scholarly impact, academic nursing programs may be better represented by Scopus, without compromising journal quality. Programs with strong interdisciplinary work should examine all areas of strength to ensure appropriate coverage. Copyright © 2017 Elsevier Inc. All rights reserved.

  15. Noxa controls Mule-dependent Mcl-1 ubiquitination through the regulation of the Mcl-1/USP9X interaction.

    Science.gov (United States)

    Gomez-Bougie, Patricia; Ménoret, Emmanuelle; Juin, Philippe; Dousset, Christelle; Pellat-Deceunynck, Catherine; Amiot, Martine

    2011-09-30

    The level of the Mcl-1 pro-survival protein is highly regulated, and the down-regulation of Mcl-1 expression favors the apoptotic process. Mcl-1 physically interacts with different BH3-only proteins; particularly, Noxa is involved in the modulation of Mcl-1 expression. In this study, we demonstrated that Noxa triggers the degradation of Mcl-1 at the mitochondria according to the exclusive location of Noxa at this compartment. The Noxa-induced degradation of Mcl-1 required the E3 ligase Mule, which is responsible for the polyubiquitination of Mcl-1. Because the USP9X deubiquitinase was recently demonstrated to be involved in Mcl-1 protein turnover by preventing its degradation through the removal of conjugated ubiquitin, we investigated whether Noxa affected the deubiquitination process. Interestingly, Noxa over-expression caused a decrease in the USP9X/Mcl-1 interaction associated with an increase in the Mcl-1 polyubiquitinated forms. Additionally, Noxa over-expression triggered an increase in the Mule/Mcl-1 interaction in parallel with the decrease in Mule/USP9X complex formation. Taken together, these modifications result in the degradation of Mcl-1 by the proteasome machinery. The implication of Noxa in the regulation of Mcl-1 proteasomal degradation adds complexity to this process, which is governed by multiple interactions.

  16. Enhancing Faculty Engagement and Student Learning in Foundational STEM Courses at a Large Public University

    Science.gov (United States)

    Jackson, Howard; Koenig, Kathleen

    2015-03-01

    Enhancing student learning requires both the strong involvement of the faculty member and the student. We present preliminary efforts of an NSF-supported multi-disciplinary program to enhance learning in foundational STEM courses. A central theme, supported by evidenced-based research across the STEM disciplines, is that active leaning engages students in ways that enhance student learning. A secondary theme is that sustained use of active learning techniques by faculty needs a supportive local culture. We describe our initial efforts with the use of Teaching and Learning Liaisons, faculty members trained in research-based instructional strategies in order to lower the barriers for faculty to try new (to them) active learning strategies, and to increase the probability that these faculty carry out the strategies with fidelity. We have assembled a collection of faculty across the STEM disciplines of Biology, Chemistry, and Physics to participate and will compare initial activities by these departments. Efforts to create a supportive culture for these faculty was also provided by tangible department head efforts We acknowledge support from the National Science Foundation (DUE-1022563).

  17. Academic freedom and academic duty to teach social justice: a perspective and pedagogy for public health nursing faculty.

    Science.gov (United States)

    Fahrenwald, Nancy L; Taylor, Janette Y; Kneipp, Shawn M; Canales, Mary K

    2007-01-01

    Public health nursing practice is rooted in the core value of social justice. Nursing faculty whose expertise is in public health are often the content experts responsible for teaching this essential, yet potentially controversial, value. Contemporary threats to academic freedom remind us that the disciplinary autonomy and academic duty to teach social justice may be construed as politically ideological. These threats are of particular concern when faculty members guide students through a scientific exploration of sociopolitical factors that lead to health-related social injustices and encourage students to improve and transform injustices in their professional careers. This article (a) reviews recent challenges to academic freedom that influence social justice education, (b) explores academic freedom and duty to teach social justice within the discipline of nursing, and (c) proposes a praxis-based approach to social justice education, which is grounded in transformative pedagogy.

  18. Scientific Publications by the Faculty of the College of Science, UP Diliman: September 1988 to May 1998

    Directory of Open Access Journals (Sweden)

    May Lim

    1998-06-01

    Full Text Available We present a comprehensive survey of the scientific publications by the Faculty (Professors, Associate Professors, and Assistant Professors of the College of Science, UP Diliman. The initial listings are obtained from UNCOVER and MEDLINE databases which are freely available in the Internet. Our search covers articles that have been published between September 1988 and May 1998. Books and conference proceedings are excluded. Performance analysis is done along academic ranks and units. Final tally considers only journals covered by the Science Citation Index. Based on our tally, no academic unit has achieved the rule of thumb for research excellence which is at least one internationally-abstracted publication per faculty per year.

  19. College Governance: A Comparison of Faculty Evaluation in Public and Private Colleges with Implications for the Improvement of the Evaluation Process at Johnson and Wales College.

    Science.gov (United States)

    Bukowski, Joseph E.

    This study focuses on selected factors in the evaluation of faculty members in: (1) colleges accredited by the Association of Independent Colleges and Schools; (2) public junior and senior colleges; and (3) Rhode Island colleges. Results of the study indicate that faculty evaluation schemes must follow the basic goals and philosophy of the…

  20. Mcl-1 Ubiquitination: Unique Regulation of an Essential Survival Protein

    Directory of Open Access Journals (Sweden)

    Barbara Mojsa

    2014-05-01

    Full Text Available Mcl-1 is an anti-apoptotic protein of the Bcl-2 family that is essential for the survival of multiple cell lineages and that is highly amplified in human cancer. Under physiological conditions, Mcl-1 expression is tightly regulated at multiple levels, involving transcriptional, post-transcriptional and post-translational processes. Ubiquitination of Mcl-1, that targets it for proteasomal degradation, allows for rapid elimination of the protein and triggering of cell death, in response to various cellular events. In the last decade, a number of studies have elucidated different pathways controlling Mcl-1 ubiquitination and degradation. Four different E3 ubiquitin-ligases (e.g., Mule, SCFβ-TrCP, SCFFbw7 and Trim17 and one deubiquitinase (e.g., USP9X, that respectively mediate and oppose Mcl-1 ubiquitination, have been formerly identified. The interaction between Mule and Mcl-1 can be modulated by other Bcl-2 family proteins, while recognition of Mcl-1 by the other E3 ubiquitin-ligases and deubiquitinase is influenced by phosphorylation of specific residues in Mcl-1. The protein kinases and E3 ubiquitin-ligases that are involved in the regulation of Mcl-1 stability vary depending on the cellular context, highlighting the complexity and pivotal role of Mcl-1 regulation. In this review, we attempt to recapitulate progress in understanding Mcl-1 regulation by the ubiquitin-proteasome system.

  1. Examining Retention and Contingent Faculty Use in a State System of Public Higher Education

    Science.gov (United States)

    Jaeger, Audrey J.; Eagan, M. Kevin

    2011-01-01

    In many cases, state systems of higher education are not only challenged to address decreasing state budgets but are also asked to increase student retention and other measures of student success. The increased use of contingent faculty may help economically, but this trend may have unintended consequences. This research used logistic regression…

  2. Education About Dental Hygienists' Roles in Public Dental Prevention Programs: Dental and Dental Hygiene Students' and Faculty Members' and Dental Hygienists' Perspectives.

    Science.gov (United States)

    Pervez, Anushey; Kinney, Janet S; Gwozdek, Anne; Farrell, Christine M; Inglehart, Marita R

    2016-09-01

    In 2005, Public Act No. 161 (PA 161) was passed in Michigan, allowing dental hygienists to practice in approved public dental prevention programs to provide services for underserved populations while utilizing a collaborative agreement with a supervising dentist. The aims of this study were to assess how well dental and dental hygiene students and faculty members and practicing dental hygienists have been educated about PA 161, what attitudes and knowledge about the act they have, and how interested they are in additional education about it. University of Michigan dental and dental hygiene students and faculty members, students in other Michigan dental hygiene programs, and dental hygienists in the state were surveyed. Respondents (response rate) were 160 dental students (50%), 63 dental hygiene students (82%), 30 dental faculty members (26%), and 12 dental hygiene faculty members (52%) at the University of Michigan; 143 dental hygiene students in other programs (20%); and 95 members of the Michigan Dental Hygienists' Association (10%). The results showed that the dental students were less educated about PA 161 than the dental hygiene students, and the dental faculty members were less informed than the dental hygiene faculty members and dental hygienists. Responding dental hygiene faculty members and dental hygienists had more positive attitudes about PA 161 than did the students and dental faculty members. Most of the dental hygiene faculty members and dental hygienists knew a person providing services in a PA 161 program. Most dental hygiene students, faculty members, and dental hygienists wanted more education about PA 161. Overall, the better educated about the program the respondents were, the more positive their attitudes, and the more interested they were in learning more.

  3. Alumni's perception of public health informatics competencies: lessons from the Graduate Program of Public Health, Faculty of Medicine, Universitas Gadjah Mada, Indonesia.

    Science.gov (United States)

    Fuad, Anis; Sanjaya, Guardian Yoki; Lazuardi, Lutfan; Rahmanti, Annisa Ristya; Hsu, Chien-Yeh

    2013-01-01

    Public health informatics has been defined as the systematic application of information and computer science and technology to public health practice, research, and learning [1]. Unfortunately, limited reports exist concerning to the capacity building strategies to improve public health informatics workforce in limited-resources setting. In Indonesia, only three universities, including Universitas Gadjah Mada (UGM), offer master degree program on related public health informatics discipline. UGM started a new dedicated master program on Health Management Information Systems in 2005, under the auspice of the Graduate Program of Public Health at the Faculty of Medicine. This is the first tracer study to the alumni aiming to a) identify the gaps between curriculum and the current jobs and b) describe their perception on public health informatics competencies. We distributed questionnaires to 114 alumni with 36.84 % response rate. Despite low response rate, this study provided valuable resources to set up appropriate competencies, curriculum and capacity building strategies of public health informatics workforce in Indonesia.

  4. Current views and practice of faculty members and consultants regarding ′Publications in India′: A cross-sectional study

    Directory of Open Access Journals (Sweden)

    Madhuri S Kurdi

    2015-01-01

    Full Text Available Background and Aims: There is an increasing enthusiasm and pressure to submit scientific articles to journals for publication due to official policies. This has led to increased stress on authors and editors and in issues like plagiarism. We planned a cross-sectional study with an aim to explore the current publication related views and practice of faculty members and consultants. Methods: We conducted a questionnaire based prospective survey with 22 questions divided into parts. Print and electronic versions were sent to around 18,270 members in total, a majority of whom were anaesthesiologists and 600 members responded to our questionnaire. A database was created and analysed using Microsoft Excel. Results: About 80% felt that online journals were better read than print journals. Eighty eight percent agreed that publications improve academic skills. The Medical Council of India requirements to publish in reputed journals were cited as the main reasons for plagiarism. The publication rule had become a burden for 46% respondents. Review articles were most likely to be read though clinical investigations were considered to be of maximum academic significance. Review/publishing time followed by author requirements and journal indexing were the points our respondents liked to see most when choosing a journal for article submission. Conclusion: Our survey results depict the current author related views and trends in publication practice which may guide in evidence-based policy making.

  5. Mcl-1 dynamics influence mitotic slippage and death in mitosis.

    Science.gov (United States)

    Sloss, Olivia; Topham, Caroline; Diez, Maria; Taylor, Stephen

    2016-02-02

    Microtubule-binding drugs such as taxol are frontline treatments for a variety of cancers but exactly how they yield patient benefit is unclear. In cell culture, inhibiting microtubule dynamics prevents spindle assembly, leading to mitotic arrest followed by either apoptosis in mitosis or slippage, whereby a cell returns to interphase without dividing. Myeloid cell leukaemia-1 (Mcl-1), a pro-survival member of the Bcl-2 family central to the intrinsic apoptosis pathway, is degraded during a prolonged mitotic arrest and may therefore act as a mitotic death timer. Consistently, we show that blocking proteasome-mediated degradation inhibits taxol-induced mitotic apoptosis in a Mcl-1-dependent manner. However, this degradation does not require the activity of either APC/C-Cdc20, FBW7 or MULE, three separate E3 ubiquitin ligases implicated in targeting Mcl-1 for degradation. This therefore challenges the notion that Mcl-1 undergoes regulated degradation during mitosis. We also show that Mcl-1 is continuously synthesized during mitosis and that blocking protein synthesis accelerates taxol induced death-in-mitosis. Modulating Mcl-1 levels also influences slippage; overexpressing Mcl-1 extends the time from mitotic entry to mitotic exit in the presence of taxol, while inhibiting Mcl-1 accelerates it. We suggest that Mcl-1 competes with Cyclin B1 for binding to components of the proteolysis machinery, thereby slowing down the slow degradation of Cyclin B1 responsible for slippage. Thus, modulating Mcl-1 dynamics influences both death-in-mitosis and slippage. However, because mitotic degradation of Mcl-1 appears not to be under the control of an E3 ligase, we suggest that the notion of network crosstalk is used with caution.

  6. Student Learning in Guatemala: An Untenured Faculty Perspective on International Service Learning and Public Good

    Science.gov (United States)

    Taylor, Matthew J.

    2009-01-01

    In this article I explain how I combined service learning, public-good work, and research in Guatemala. This path has not been easy. Indeed, it has been risky because the time invested in public-good work and teaching field classes could have detracted from research productivity. Taking a risk under the current and traditional academic model at…

  7. Flexibility for Survival: State Funding and Contingent Faculty Employment at Public Higher Education Institutions

    Science.gov (United States)

    Frye, Joanna R.

    2015-01-01

    The dynamics of state funding for public higher education in the United States are changing. Per-student state appropriations to higher education have decreased over the past few decades and have become increasingly volatile from year to year. As public higher education institutions seek ways to educate more students with fewer and less…

  8. High-quality NMR structure of human anti-apoptotic protein domain Mcl-1(171-327 for cancer drug design.

    Directory of Open Access Journals (Sweden)

    Gaohua Liu

    Full Text Available A high-quality NMR solution structure is presented for protein hMcl-1(171-327 which comprises residues 171-327 of the human anti-apoptotic protein Mcl-1 (hMcl-1. Since this construct contains the three Bcl-2 homology (BH sequence motifs which participate in forming a binding site for inhibitors of hMcl-1, it is deemed to be crucial for structure-based design of novel anti-cancer drugs blocking the Mcl1 related anti-apoptotic pathway. While the coordinates of an NMR solution structure for a corresponding construct of the mouse homologue (mMcl-1 are publicly available, our structure is the first atomic resolution structure reported for the 'apo form' of the human protein. Comparison of the two structures reveals that hMcl-1(171-327 exhibits a somewhat wider ligand/inhibitor binding groove as well as a different charge distribution within the BH3 binding groove. These findings strongly suggest that the availability of the human structure is of critical importance to support future design of cancer drugs.

  9. A Case Study: Faculty Perceptions of the Challenges and Successes in Experiential Learning at a Public University

    Science.gov (United States)

    Tuberville, Kathy A.

    2014-01-01

    Faculty mentoring has been identified as an important component of experiential learning success. However, most higher education institutions lack the support to provide training and guidance to faculty for this type of instructional programming. The identified purpose of the conducted exploratory study was to explore the faculty perceptions…

  10. Obstacles Faced by Heads of Departments and Faculty Members in the Jordanian Public Universities in the Implementation of Vocational and Technical Education Programs from Their Perspective

    Science.gov (United States)

    Hammad, Heba Ibraheem; Airout, Mostafa Mohammad

    2016-01-01

    The purpose of the study is to find out the obstacles faced by heads of departments and faculty members at Jordanian public universities in the implementation of vocational and technical education programs from their perspective, and to find out the effect of gender, experience, and academic rank on their perspective. To achieve the aim of the…

  11. Prototypes of Student Veterans with Post Traumatic Stress Disorder and Traumatic Brain Injury among Faculty in Illinois Public Four-Year Universities

    Science.gov (United States)

    Taylor, Kathy J.

    2013-01-01

    One of the primary reasons many college students with disabilities, and more specifically college student veterans with disabilities, do not seek support services is due to the stigma associated with disability, especially cognitive and mental health disabilities. The purpose of the present study was to explore how public university faculty in the…

  12. A Report Card on Latina/o Leadership in California's Public Universities: A Trend Analysis of Faculty, Students, and Executives in the CSU and UC Systems

    Science.gov (United States)

    Santos, Jose L.; Acevedo-Gil, Nancy

    2013-01-01

    The article examines the status of leadership in two California public higher education systems: California State University (CSU) and the University of California (UC) from 2001 to 2009. Findings reveal that the representation of Latina/o faculty and administrators does not reflect the density in the Latina/o undergraduate student and general…

  13. Prototypes of Student Veterans with Post Traumatic Stress Disorder and Traumatic Brain Injury among Faculty in Illinois Public Four-Year Universities

    Science.gov (United States)

    Taylor, Kathy J.

    2013-01-01

    One of the primary reasons many college students with disabilities, and more specifically college student veterans with disabilities, do not seek support services is due to the stigma associated with disability, especially cognitive and mental health disabilities. The purpose of the present study was to explore how public university faculty in the…

  14. Targeting Mcl-1 for Radiosensitization of Pancreatic Cancers

    Directory of Open Access Journals (Sweden)

    Dongping Wei

    2015-02-01

    Full Text Available In order to identify targets whose inhibition may enhance the efficacy of chemoradiation in pancreatic cancer, we previously conducted an RNAi library screen of 8,800 genes. We identified Mcl-1 (myeloid cell leukemia-1, an anti-apoptotic member of the Bcl-2 family, as a target for sensitizing pancreatic cancer cells to chemoradiation. In the present study we investigated Mcl-1 inhibition by either genetic or pharmacological approaches as a radiosensitizing strategy in pancreatic cancer cells. Mcl-1 depletion by siRNA produced significant radiosensitization in BxPC-3 and Panc-1 cells in association with Caspase-3 activation and PARP cleavage, but only minimal radiosensitization in MiaPaCa-2 cells. We next tested the ability of the recently identified, selective, small molecule inhibitor of Mcl-1, UMI77, to radiosensitize in pancreatic cancer cells. UMI77 caused dissociation of Mcl-1 from the pro-apoptotic protein Bak and produced significant radiosensitization in BxPC-3 and Panc-1 cells, but minimal radiosensitization in MiaPaCa-2 cells. Radiosensitization by UMI77 was associated with Caspase-3 activation and PARP cleavage. Importantly, UMI77 did not radiosensitize normal small intestinal cells. In contrast, ABT-737, an established inhibitor of Bcl-2, Bcl-XL, and Bcl-w, failed to radiosensitize pancreatic cancer cells suggesting the unique importance of Mcl-1 relative to other Bcl-2 family members to radiation survival in pancreatic cancer cells. Taken together, these results validate Mcl-1 as a target for radiosensitization of pancreatic cancer cells and demonstrate the ability of small molecules which bind the canonical BH3 groove of Mcl-1, causing displacement of Mcl-1 from Bak, to selectively radiosensitize pancreatic cancer cells.

  15. Targeting Mcl-1 for multiple myeloma (MM) therapy: drug-induced generation of Mcl-1 fragment Mcl-1(128-350) triggers MM cell death via c-Jun upregulation.

    Science.gov (United States)

    Fan, Fengjuan; Tonon, Giovanni; Bashari, Muhammad Hasan; Vallet, Sonia; Antonini, Elena; Goldschmidt, Hartmut; Schulze-Bergkamen, Henning; Opferman, Joseph T; Sattler, Martin; Anderson, Kenneth C; Jäger, Dirk; Podar, Klaus

    2014-02-28

    Myeloid cell leukemia-1 (Mcl-1, HGNC: 6943), a pro-survival member of the Bcl-2 family, plays a crucial role in Multiple Myeloma (MM) pathogenesis and drug resistance, thus representing a promising therapeutic target in MM. A novel strategy to inhibit Mcl-1 activity is the induction of ubiquitin-independent Mcl-1 degradation. Our own and other previous studies have demonstrated caspase-dependent generation of a 28kDa Mcl-1 fragment, Mcl-1(128-350), which inhibits MM cell proliferation and survival. Here, we show that similar to bortezomib, the novel proteasome inhibitors carfilzomib and ixazomib, as well as staurosporine and adaphostin, induce the generation of Mcl-1(128-350) in MM cells. Next, the molecular sequelae downstream of Mcl-1(128-350), which mediate its pro-apoptotic activity, were delineated. Surprisingly, we observed nuclear accumulation of drug-induced or exogenously overexpressed Mcl-1(128-350), followed by elevated mRNA and protein levels of c-Jun, as well as enhanced AP-1 reporter activity. Moreover, drug-induced AP-1 activity was blocked after introducing a point mutation into the highly conserved Mcl-1 caspase-cleavage site Asp127, but not Asp157. Consequently, drug-triggered cell death was significantly decreased in MM cells transfected with Mcl-1 D127A, but not with Mcl-1 D157A. Consistent with these data, treatment with bortezomib triggered c-Jun upregulation followed by apoptosis in Mcl-1(wt/wt), but not Mcl-1(Δ/null) murine embryonic fibroblasts (MEFs). Transfection of a plasmid carrying Mcl-1(wt) into Mcl-1(Δ/null) MEFs restored bortezomib-induced Mcl-1 fragmentation, c-Jun upregulation and AP-1 reporter activity. Finally, our data indicate that drug-induced generation of a pro-apoptotic Mcl-1 fragment followed by c-Jun upregulation may also be a novel therapeutic approach in other tumor entities.

  16. The Human Capital of Knowledge Brokers: An analysis of attributes, capacities and skills of academic teaching and research faculty at Kenyan schools of public health.

    Science.gov (United States)

    Jessani, Nasreen; Kennedy, Caitlin; Bennett, Sara

    2016-08-02

    Academic faculty involved in public health teaching and research serve as the link and catalyst for knowledge synthesis and exchange, enabling the flow of information resources, and nurturing relations between 'two distinct communities' - researchers and policymakers - who would not otherwise have the opportunity to interact. Their role and their characteristics are of particular interest, therefore, in the health research, policy and practice arena, particularly in low- and middle-income countries. We investigated the individual attributes, capacities and skills of academic faculty identified as knowledge brokers (KBs) in schools of public health (SPH) in Kenya with a view to informing organisational policies around the recruitment, retention and development of faculty KBs. During April 2013, we interviewed 12 academics and faculty leadership (including those who had previously been identified as KBs) from six SPHs in Kenya, and 11 national health policymakers with whom they interact. Data were qualitatively analyzed using inductive thematic analysis to unveil key characteristics. Key characteristics of KBs fell into five categories: sociodemographics, professional competence, experiential knowledge, interactive skills and personal disposition. KBs' reputations benefitted from their professional qualifications and content expertise. Practical knowledge in policy-relevant situations, and the related professional networks, allowed KB's to navigate both the academic and policy arenas and also to leverage the necessary connections required for policy influence. Attributes, such as respect and a social conscience, were also important KB characteristics. Several changes in Kenya are likely to compel academics to engage increasingly with policymakers at an enhanced level of debate, deliberation and discussion in the future. By recognising existing KBs, supporting the emergence of potential KBs, and systematically hiring faculty with KB-specific characteristics, SPHs can

  17. Reflections on a decade of research by ASEAN dental faculties: analysis of publications from ISI-WOS databases from 2000 to 2009.

    Science.gov (United States)

    Sirisinha, Stitaya; Koontongkaew, Sittichai; Phantumvanit, Prathip; Wittayawuttikul, Ruchareka

    2011-05-01

    This communication analyzed research publications in dentistry in the Institute of Scientific Information Web of Science databases of 10 dental faculties in the Association of South-East Asian Nations (ASEAN) from 2000 to 2009. The term used for the "all-document types" search was "Faculty of Dentistry/College of Dentistry." Abstracts presented at regional meetings were also included in the analysis. The Times Higher Education System QS World University Rankings showed that universities in the region fare poorly in world university rankings. Only the National University of Singapore and Nanyang Technological University appeared in the top 100 in 2009; 19 universities in the region, including Indonesia, Malaysia, the Philippines, Singapore, and Thailand, appeared in the top 500. Data from the databases showed that research publications by dental institutes in the region fall short of their Asian counterparts. Singapore and Thailand are the most active in dental research of the ASEAN countries.

  18. Designing and Implementing Content-Based Courses in English with a Non-Language Faculty at a Public Colombian University

    National Research Council Canada - National Science Library

    Fabio Alberto Arismendi Gómez; Claudia Patricia Díaz Mosquera; Leidy Natalia Salazar Valencia

    2008-01-01

    ... participated in a multi-site study to implement content-based (CB) courses in English. The professors, who had a high level of proficiency in English, worked in collaboration with language faculty...

  19. PBL triggers in relation to students' generated learning issues and predetermined faculty objectives: Study in a Malaysian public university.

    Science.gov (United States)

    Ruslai, Nurul Hidayati; Salam, Abdus

    2016-01-01

    Foundational elements of problem based learning (PBL) are triggers, tutors and students. Ineffective triggers are important issues for students' inability to generate appropriate learning issues. The objective of this study was to evaluate PBL triggers and to determine similarities of students' generated learning issues with predetermined faculty objectives. It was a retrospective study conducted in 2014 analyzing all 24 PBL-triggers used at Centre for Foundation Studies, International Islamic University Malaysia, in four semesters during two consecutive years 2011 and 2012. Triggers were used as textual and illustration format equally in each semester. Total 16 PBL-triggers with highest and lowest achieving similarities of learning issues with predetermined faculty objectives were selected equally from each semester and format. The trigger quality and learning issues related to predetermine faculty objectives were analyzed and presented as mean and percent distribution. Mean similarities score of students' generated learning issues were 3.4 over 5 predetermined faculty objectives which was 68%, varied from 58% to 79%. More than 70% similarities were generated from five textual and four illustrated triggers, while PBL, it is the designing considering influential variables that influence higher outcomes. Triggers should have planned clues that lead students to generate issues correlate with faculty objectives. Educational institution should emphasize on training needs of faculty at regular interval to develop and re-in force teachers' skills in trigger design, thereby to promote a sustainable educational and organizational development.

  20. Factors Influencing Faculty Participation in Internationalization at the University of Minnesota's Schools of Nursing and Public Health: A Case Study

    Science.gov (United States)

    Beatty, Matthew R.

    2013-01-01

    The internationalization of higher education is no longer a desirable academic ideal. Rather, it is an essential component for higher education. In the era of globalization, colleges and universities are deploying widespread initiatives to infuse a global dimension into their teaching, research and service functions. Faculty play an important role…

  1. A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1.

    Directory of Open Access Journals (Sweden)

    Matthew C Clifton

    Full Text Available Crystallization of a maltose-binding protein MCL1 fusion has yielded a robust crystallography platform that generated the first apo MCL1 crystal structure, as well as five ligand-bound structures. The ability to obtain fragment-bound structures advances structure-based drug design efforts that, despite considerable effort, had previously been intractable by crystallography. In the ligand-independent crystal form we identify inhibitor binding modes not observed in earlier crystallographic systems. This MBP-MCL1 construct dramatically improves the structural understanding of well-validated MCL1 ligands, and will likely catalyze the structure-based optimization of high affinity MCL1 inhibitors.

  2. Deubiquitinase USP9x Confers Radioresistance through Stabilization of Mcl-1

    Directory of Open Access Journals (Sweden)

    Donatella Trivigno

    2012-10-01

    Full Text Available Myeloid cell leukemia sequence 1 (Mcl-1, an antiapoptotic member of the Bcl-2 family, is often overexpressed in tumor cells limiting the therapeutic success. Mcl-1 differs from other Bcl-2 members by its high turnover rate. Its expression level is tightly regulated by ubiquitylating and deubiquitylating enzymes. Interaction of Mcl-1 with certain Bcl-2 homology domain 3 (BH3-only members of the Bcl-2 family can limit the access to Mcl-1 ubiquitin ligase E3 and stabilizes the antiapoptotic protein. In addition, the overexpression of the deubiquitinase ubiquitin-specific protease 9x (USP9x can result in the accumulation of Mcl-1 by removing poly-ubiquitin chains from Mcl-1 preventing its proteasomal degradation. Analyzing radiation-induced apoptosis in Jurkat cells, we found that Mcl-1 was downregulated more efficiently in sensitive parental cells than in a resistant subclone. The decline of Mcl-1 correlated with cell death induction and clonogenic survival. Knockdown of BH3-only proteins Bim, Puma, and Noxa did not affect Mcl-1 level or radiation-induced apoptosis. However, ionizing radiation resulted in activation of USP9x and enhanced deubiquitination of Mcl-1 in the radioresistant cells preventing fast Mcl-1 degradation. USP9x knockdown enhanced radiation-induced decrease of Mcl-1 and sensitized the radioresistant cells to apoptosis induction, whereas USP9x knockdown alone did not change Mcl-1 level in unirradiated cells. Together, our results indicate that radiation-induced activation of USP9x inhibits Mcl-1 degradation and apoptosis resulting in increased radioresistance.

  3. Metformin enhances TRAIL-induced apoptosis by Mcl-1 degradation via Mule in colorectal cancer cells

    Science.gov (United States)

    Kim, Jung Lim; Kim, Bo Ram; Na, Yoo Jin; Jo, Min Jee; Jeong, Yoon A.; Lee, Suk-Young; Lee, Sun Il; Lee, Yong Yook; Oh, Sang Cheul

    2016-01-01

    Metformin is an anti-diabetic drug with a promising anti-cancer potential. In this study, we show that subtoxic doses of metformin effectively sensitize human colorectal cancer (CRC) cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), which induces apoptosis. Metformin alone did not induce apoptosis, but significantly potentiated TRAIL-induced apoptosis in CRC cells. CRC cells treated with metformin and TRAIL showed activation of the intrinsic and extrinsic pathways of caspase activation. We attempted to elucidate the underlying mechanism, and found that metformin significantly reduced the protein levels of myeloid cell leukemia 1 (Mcl-1) in CRC cells and, the overexpression of Mcl-1 inhibited cell death induced by metformin and/or TRAIL. Further experiments revealed that metformin did not affect mRNA levels, but increased proteasomal degradation and protein stability of Mcl-1. Knockdown of Mule triggered a significant decrease of Mcl-1 polyubiquitination. Metformin caused the dissociation of Noxa from Mcl-1, which allowed the binding of the BH3-containing ubiquitin ligase Mule followed by Mcl-1ubiquitination and degradation. The metformin-induced degradation of Mcl-1 required E3 ligase Mule, which is responsible for the polyubiquitination of Mcl-1. Our study is the first report indicating that metformin enhances TRAIL-induced apoptosis through Noxa and favors the interaction between Mcl-1 and Mule, which consequently affects Mcl-1 ubiquitination. PMID:27517746

  4. c-Myc dependent expression of pro-apoptotic Bim renders HER2-overexpressing breast cancer cells dependent on anti-apoptotic Mcl-1

    Directory of Open Access Journals (Sweden)

    Jézéquel Pascal

    2011-09-01

    Full Text Available Abstract Background Anti-apoptotic signals induced downstream of HER2 are known to contribute to the resistance to current treatments of breast cancer cells that overexpress this member of the EGFR family. Whether or not some of these signals are also involved in tumor maintenance by counteracting constitutive death signals is much less understood. To address this, we investigated what role anti- and pro-apoptotic Bcl-2 family members, key regulators of cancer cell survival, might play in the viability of HER2 overexpressing breast cancer cells. Methods We used cell lines as an in vitro model of HER2-overexpressing cells in order to evaluate how anti-apoptotic Bcl-2, Bcl-xL and Mcl-1, and pro-apoptotic Puma and Bim impact on their survival, and to investigate how the constitutive expression of these proteins is regulated. Expression of the proteins of interest was confirmed using lysates from HER2-overexpressing tumors and through analysis of publicly available RNA expression data. Results We show that the depletion of Mcl-1 is sufficient to induce apoptosis in HER2-overexpressing breast cancer cells. This Mcl-1 dependence is due to Bim expression and it directly results from oncogenic signaling, as depletion of the oncoprotein c-Myc, which occupies regions of the Bim promoter as evaluated in ChIP assays, decreases Bim levels and mitigates Mcl-1 dependence. Consistently, a reduction of c-Myc expression by inhibition of mTORC1 activity abrogates occupancy of the Bim promoter by c-Myc, decreases Bim expression and promotes tolerance to Mcl-1 depletion. Western blot analysis confirms that naïve HER2-overexpressing tumors constitutively express detectable levels of Mcl-1 and Bim, while expression data hint on enrichment for Mcl-1 transcripts in these tumors. Conclusions This work establishes that, in HER2-overexpressing tumors, it is necessary, and maybe sufficient, to therapeutically impact on the Mcl-1/Bim balance for efficient induction of

  5. 15-year follow-up of the Second Nordic Mantle Cell Lymphoma trial (MCL2)

    DEFF Research Database (Denmark)

    Eskelund, Christian W.; Kolstad, Arne; Jerkeman, Mats;

    2016-01-01

    , developed by the Nordic Lymphoma Group. We here present the 15-year updated results of the Nordic MCL2 study after a median follow-up of 11·4 years: For all patients on an intent-to-treat basis, the median overall and progression-free survival was 12·7 and 8·5 years, respectively. The MCL International......In recent decades, the prognosis of Mantle Cell Lymphoma (MCL) has been significantly improved by intensified first-line regimens containing cytarabine, rituximab and consolidation with high-dose-therapy and autologous stem cell transplantation. One such strategy is the Nordic MCL2 regimen...

  6. Structural Insights into the Degradation of Mcl-1 Induced by BH3 Domains

    Energy Technology Data Exchange (ETDEWEB)

    Czabotar,P.; Lee, E.; van Delft, M.; Day, C.; Smith, B.; Huang, D.; Fairlie, W.; Hinds, M.; Colman, P.

    2007-01-01

    Apoptosis is held in check by prosurvival proteins of the Bcl-2 family. The distantly related BH3-only proteins bind to and antagonize them, thereby promoting apoptosis. Whereas binding of the BH3-only protein Noxa to prosurvival Mcl-1 induces Mcl-1 degradation by the proteasome, binding of another BH3-only ligand, Bim, elevates Mcl-1 protein levels. We compared the three-dimensional structures of the complexes formed between BH3 peptides of both Bim and Noxa, and we show that a discrete C-terminal sequence of the Noxa BH3 is necessary to instigate Mcl-1 degradation.

  7. The Role of Mcl-1 in S. aureus-Induced Cytoprotection of Infected Macrophages

    Directory of Open Access Journals (Sweden)

    Joanna Koziel

    2013-01-01

    cytoprotection of infected cells leading to apoptosis. Increased MCL1 expression in infected cells was associated with enhanced NFκB activation and subsequent IL-6 secretion, since the inhibition of both NFκB and IL-6 signalling pathways abrogated Mcl-1 induction and cytoprotection. Finally, we confirmed our observation in vivo in murine model of septic arthritis showing the association between the severity of arthritis and Mcl-1 expression. Therefore, we propose that S. aureus is hijacking the Mcl-1-dependent inhibition of apoptosis to prevent the elimination of infected host cells, thus allowing the intracellular persistence of the pathogen, its dissemination by infected macrophages, and the progression of staphylococci diseases.

  8. Faculty Members on Boards of Trustees

    Science.gov (United States)

    Ehrenberg, Ronald G.; Patterson, Richard W.; Key, Andrew V.

    2013-01-01

    During the 2011-12 academic year, a group of faculty and student researchers at the Cornell Higher Education Research Institute (CHERI) gathered information on which public and private institutions had faculty members on boards of trustees and obtained the names of the faculty members serving in these roles. In April and May 2012, the authors…

  9. Mouse Noxa uses only the C-terminal BH3-domain to inactivate Mcl-1.

    Science.gov (United States)

    Weber, Arnim; Ausländer, David; Häcker, Georg

    2013-09-01

    Noxa is a member of the pro-apoptotic BH3-only group of Bcl-2 proteins that is known to bind specifically to anti-apoptotic Mcl-1 and A1, antagonizing their function. Mcl-1 has been reported to have a short half-life, and Noxa up-regulation accelerates Mcl-1 degradation by the proteasome. Unlike human Noxa, mouse Noxa has two BH3-domains, which both have affinity for Mcl-1. We here investigate two aspects of the molecular function of Noxa, namely the requirements for the two BH3-domains in mouse Noxa and the role of Noxa in Mcl-1-degradation. We found that only the C-terminal BH3-domain of mouse Noxa is active in neutralizing Mcl-1. This was the result of the targeting of Noxa to the outer mitochondrial membrane through its C-terminal alpha-helix, which allowed Mcl-1-neutralization only when the BH3-domain was immediately N-terminal of the membrane anchor. However, the N-terminal BH3-domain enhanced interaction with Mcl-1 and A1. The Noxa-dependent degradation of Mcl-1 was independent of the kinase GSK3 and the deubiquitinase Usp9x in mouse embryonic fibroblasts. These data show that Noxa is targeted to the mitochondrial membrane where it neutralises Mcl-1 via its C-terminal BH3-domain and suggest that Noxa is co-degraded with Noxa, in a way independent of ubiquitin-modifying enzymes described for Mcl-1.

  10. Enhancing Evidence-Informed Decision Making: Strategies for Engagement between Public Health Faculty and Policymakers in Kenya

    Science.gov (United States)

    Jessani, Nasreen; Kennedy, Caitlin; Bennett, Sara

    2017-01-01

    This article examines the complex interactions and strategies for engagement--both existing as well as desired--between academic Knowledge Brokers (KBs) and national health policymakers in Kenya. Based on semi-structured interviews with academic KBs and university leaders from six Schools of Public Health (SPHs) as well as national policymakers,…

  11. Is intra-articular pathology associated with MCL edema on MR imaging of the non-traumatic knee?

    Energy Technology Data Exchange (ETDEWEB)

    Blankenbaker, Donna G.; De Smet, Arthur A. [University of Wisconsin Medical School, Division of Musculoskeletal Imaging, Department of Radiology, Madison (United States); Fine, Jason P. [University of Wisconsin, Department of Statistics, Madison (United States); University of Wisconsin, Department of Biostatistics and Informatics, Madison (United States)

    2005-08-01

    Edema surrounding the medial collateral ligament (MCL) is seen on MR imaging in patients with MCL injuries and in patients with radiographic osteoarthritis in the non-traumatic knee. Because we noted MCL edema in patients without prior trauma or osteoarthritis, we studied the association between intra-articular pathology and MCL edema in patients without knee trauma. We evaluated the MR examinations of 247 consecutive patients (121 male, 126 female with a mean age of 44 years) without recent trauma for the presence of edema surrounding the MCL, meniscal and ACL tears, medial meniscal extrusion, medial compartment chondromalacia, and osteoarthritis. The percentages of patients illustrating MCL edema with and without each type of pathology were compared using Fisher's exact test to determine if there was a statistically significant association. We found MCL edema in 60% of 247 patients. MCL edema was present in 67% of patients with medial meniscal tears, 35% with lateral meniscal tears, 100% with meniscal extrusion of 3 mm or more, 78% with femoral chondromalacia, 82% with tibial chondromalacia, and 50% with osteoarthritis. The percentage of patients with edema increased with the severity of the chondromalacia. These associations were all statistically significant (p <0.02). The mean age of those with MCL edema was 49.7 years compared with 34.9 years without MCL edema (p <0.001). Patient gender and ACL tear did not correlate with MCL edema. Nine (4%) of the 247 patients had MCL edema without intra-articular pathology. None of these 9 patients had MCL tenderness or joint laxity on physical examination. We confirmed that MCL edema is associated with osteoarthritis, but is also associated with meniscal tears, meniscal extrusion, and chondromalacia. In addition, MCL edema can be seen in patients without intra-articular pathology, recent trauma or MCL abnormality on physical examination. (orig.)

  12. College Presidents' Role Performance and Faculty Satisfaction

    Science.gov (United States)

    Paxton, Dan R.; Thomas, Darwin L.

    1977-01-01

    Data gathered from 896 faculty members from two technical colleges, three community colleges, two private universities, and three public universities revealed three dimensions of the presidential role: personal-public image, faculty and student interaction with presidents, and absence of autocratic leadership style. (Author/LBH)

  13. [Expression of MCL-1 and BAK proteins in nutritional anemia and its clinical significance].

    Science.gov (United States)

    Guo, Yu-Jie; Wang, Yan; Lin, Feng-Ru

    2012-12-01

    This study was aimed to investigate the relation of MCL-1 and BAK proteins with incidence and development of nutritional anemia (NA) and their clinical significance. The MCL-1 and BAK protein levels in serum of 66 patients with NA were determined by using ELISA. Eighteen healthy people were randomly selected as normal controls. The results indicated that: (1) as compared with normal control group, the expression level of MCL-1 protein in 3 NA groups (iron-deficiency anemia, macrocytic anemia, mixed anemia) significantly decreased (P < 0.001), while the expression level of BAK protein obviously increased (P < 0.001), but the expression level of MCL-1 and BAK proteins among 3 NA groups showed no obvious differences; (2) the MCL-1 protein expression level increased and BAK protein expression level decreased in 3 NA groups after treatment (P < 0.05). (3) there was negative correlation of expression levels of MCL-1 protein with BAK protein in NA group (r = -0.858 P < 0.05). It is concluded that the MCL-1 and BAK proteins may play an important role in the incidence and development of NA, and can be used as the assist index for defining diagnosis and evaluate prognosis of NA.

  14. Suppression of Mcl-1 induces apoptosis in mouse peritoneal macrophages infected with Mycobacterium tuberculosis.

    Science.gov (United States)

    Wang, Fei-Yu; Wang, Xin-Min; Wang, Chan; Wang, Xiao-Fang; Zhang, Yu-Qing; Wu, Jiang-Dong; Wu, Fang; Zhang, Wan-Jiang; Zhang, Le

    2016-04-01

    The effect of myeloid cell leukemia-1 (Mcl-1) inhibition on apoptosis of peritoneal macrophages in mice infected with Mycobacterium tuberculosis was investigated and the primary signaling pathway associated with the transcriptional regulation of Mcl-1 was identified. Real-time PCR and western blotting indicated that Mcl-1 transcript and protein expression are upregulated during infection with virulent M. tuberculosis H37Rv and Xinjiang strains but not with attenuated M. tuberculosis strain H37Ra or Bacillus Calmette-Guérin. Mcl-1 transcript and protein expression were downregulated by specific inhibitors of the Janus kinase/signal transducer and activator of transcription (JAK/STAT), mitogen-activated protein kinase (MAPK) and phosphoinositol 3-kinase (PI3K) pathways (AG490, PD98059 and LY294002, respectively). The strongest inhibitor of Mcl-1 expression was PD98059, the MAPK inhibitor. Flow cytometry demonstrated that the rate of apoptosis in peritoneal macrophages is significantly higher in mice infected with M. tuberculosis and the rate of apoptosis is correlated with the virulence of the strain of M. tuberculosis. Apoptosis was found to be upregulated by AG490, PD98059 and LY294002, whereas inhibition of the MAPK pathway sensitized the infected macrophages to apoptosis. Taken together, these results suggest that specific downregulation of Mcl-1 significantly increases apoptosis of peritoneal macrophages and that the MAPK signaling pathway is the primary mediator of Mcl-1 expression.

  15. Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of Bax.

    Science.gov (United States)

    Cheng, Senping; Gao, Ning; Zhang, Zhuo; Chen, Gang; Budhraja, Amit; Ke, Zunji; Son, Young-ok; Wang, Xin; Luo, Jia; Shi, Xianglin

    2010-12-01

    To investigate the in vivo antitumor efficacy of quercetin in U937 xenografts and the functional roles of Mcl-1 and Bax in quercetin-induced apoptosis in human leukemia. Leukemia cells were treated with quercetin, after which apoptosis, Mcl-1 expression, and Bax activation and translocation were evaluated. The efficacy of quercetin as well as Mcl-1 expression and Bax activation were investigated in xenografts of U937 cells. Administration of quercetin caused pronounced apoptosis in both transformed and primary leukemia cells but not in normal blood peripheral mononuclear cells. Quercetin-induced apoptosis was accompanied by Mcl-1 downregulation and Bax conformational change and mitochondrial translocation that triggered cytochrome c release. Knockdown of Bax by siRNA reversed quercetin-induced apoptosis and abrogated the activation of caspase and apoptosis. Ectopic expression of Mcl-1 attenuated quercetin-mediated Bax activation, translocation, and cell death. Conversely, interruption of Mcl-1 by siRNA enhanced Bax activation and translocation, as well as lethality induced by quercetin. However, the absence of Bax had no effect on quercetin-mediated Mcl-1 downregulation. Furthermore, in vivo administration of quercetin attenuated tumor growth in U937 xenografts. The TUNEL-positive apoptotic cells in tumor sections increased in quercetin-treated mice as compared with controls. Mcl-1 downregulation and Bax activation were also observed in xenografts. These data suggest that quercetin may be useful for the treatment of leukemia by preferentially inducing apoptosis in leukemia versus normal hematopoietic cells through a process involving Mcl-1 downregulation, which, in turn, potentiates Bax activation and mitochondrial translocation, culminating in apoptosis. ©2010 AACR.

  16. Overexpression of Mcl-1L Splice Variant Is Associated with Poor Prognosis and Chemoresistance in Oral Cancers

    Science.gov (United States)

    Palve, Vinayak; Mallick, Sanchita; Ghaisas, Gauri; Kannan, Sadhana; Teni, Tanuja

    2014-01-01

    Background Altered expression of Mcl-1, an anti-apoptotic member of the Bcl-2 family, has been linked to the progression and outcome of a variety of malignancies. We have previously reported the overexpression of Mcl-1 protein in human oral cancers. The present study aimed to evaluate the clinicopathological significance of the expression of three known Mcl-1 isoforms in oral tumors and the effect of targeting Mcl-1L isoform on chemosensitivity of oral cancer cells. Methods The expression of Mcl-1 isoforms- Mcl-1L, Mcl-1S & Mcl-1ES was analyzed in 130 paired oral tumors and 9 oral cell lines using quantitative real-time PCR & protein by western blotting. The Mcl-1 mRNA levels were correlated with clinicopathological parameters and outcome of oral cancer patients. The effect of Mcl-1L shRNA or Obatoclax (a small molecule Mcl-1 inhibitor), in combination with Cisplatin on chemosensitivity of oral cancer cells was also assessed. Results Anti-apoptotic Mcl-1L was predominantly expressed, over low or undetectable pro-apoptotic Mcl-1S and Mcl-1ES isoforms. The Mcl-1L transcripts were significantly overexpressed in all cancer cell lines and in 64% oral tumors versus adjacent normals (P<0.02). In oral cancer patients, high Mcl-1L expression was significantly associated with node positivity (P = 0.021), advanced tumor size (P = 0.013) and poor overall survival (P = 0.002). Multivariate analysis indicated Mcl-1L to be an independent prognostic factor for oral cancers (P = 0.037). Mcl-1L shRNA knockdown or its inhibition by Obatoclax in combination with Cisplatin synergistically reduced viability and growth of oral cancer cells than either treatment alone. Conclusion Our studies suggest that overexpression of Mcl-1L is associated with poor prognosis and chemoresistance in oral cancers. Mcl-1L is an independent prognostic factor and a potential therapeutic target in oral cancers. PMID:25409302

  17. Faculty-Student Collaboration: Issues and Recommendations

    Directory of Open Access Journals (Sweden)

    Angeline L. Barretta-Herman

    2000-12-01

    Full Text Available This exploratory qualitative study of 11 social work faculty identified the benefits and risks of faculty-student collaboration. Benefits articulated include helping students learn to write for publication, learning the publication process, getting innovative student material published, and enriching the project through shared problem-solving. The benefits, however, must be weighed against the risks of exploitation of the student collaborator. Successful faculty-student collaboration in this dual relationship demands that faculty take responsibility for safeguarding boundaries, following the NASW Code of Ethics, and openly negotiating roles, tasks, workload, and order of authorship with the student.

  18. Molecular remission is an independent predictor of clinical outcome in patients with mantle cell lymphoma after combined immunochemotherapy: a European MCL intergroup study

    DEFF Research Database (Denmark)

    Pott, Christiane; Hoster, Eva; Delfau-Larue, Marie-Helene

    2010-01-01

    The prognostic impact of minimal residual disease (MRD) was analyzed in 259 patients with mantle cell lymphoma (MCL) treated within 2 randomized trials of the European MCL Network (MCL Younger and MCL Elderly trial). After rituximab-based induction treatment, 106 of 190 evaluable patients (56...

  19. Molecular remission is an independent predictor of clinical outcome in patients with mantle cell lymphoma after combined immunochemotherapy: a European MCL intergroup study

    DEFF Research Database (Denmark)

    Pott, Christiane; Hoster, Eva; Delfau-Larue, Marie-Helene;

    2010-01-01

    The prognostic impact of minimal residual disease (MRD) was analyzed in 259 patients with mantle cell lymphoma (MCL) treated within 2 randomized trials of the European MCL Network (MCL Younger and MCL Elderly trial). After rituximab-based induction treatment, 106 of 190 evaluable patients (56%) a...

  20. Faculty Attitudes toward Male Revenue and Nonrevenue Student-Athletes.

    Science.gov (United States)

    Engstrom, Cathy McHugh; And Others

    1995-01-01

    This study of attitudes of a random sample of faculty at a major, public, research university in the East indicated that faculty perceived male revenue and nonrevenue athletes negatively in situations dealing with athletic competence, special services, and recognition. (JPS)

  1. Participation of nursing faculty in university governance.

    Science.gov (United States)

    Bahrawy, A A

    1992-03-01

    It has been suggested that faculty participation in governance in American colleges is low, and that faculty in schools of nursing are particularly unlikely to be involved in governance activities. This study was designed to determine actual and ideal levels of nursing faculty participation in five areas of governance: academic, student, personnel, public, and financial affairs. A survey of nursing faculty suggested that they were involved substantially in academic affairs, but less involved in the other areas of governance. Generally, the faculty indicated satisfaction with their high level of participation in academic affairs, and with their lower level of participation in student affairs, personnel affairs, and public affairs; the faculty did indicate dissatisfaction with their low level of participation in financial affairs.

  2. A conceptual model for faculty development in academic medicine: the underrepresented minority faculty experience.

    Science.gov (United States)

    Daley, Sandra P; Broyles, Shelia L; Rivera, Lourdes M; Brennan, Jesse J; Lu, Ethel Regis; Reznik, Vivian

    2011-01-01

    In May 2010, the Association of American Medical Colleges reported that nonwhite professors have a lower promotion rate than white professors. A cohort of 30 underrepresented minority (URM) junior faculty who participated in a structured faculty development program at a public, research-intensive, academic medical center were followed in a 10-year longitudinal study. This paper reports on the career status of 12 of the 30 URM faculty who were eligible for promotion during this period. Ninety-two percent (11/12) of URM faculty eligible for promotion were promoted to associate professor. When asked what factors contributed to their success, these URM faculty identified access and support of senior faculty mentors, peer networking, professional skill development, and knowledge of institutional culture. A faculty development program that addresses these components can promote the success of URM faculty in academic medicine.

  3. Noxa/Mcl-1 balance regulates susceptibility of cells to camptothecin-induced apoptosis.

    Science.gov (United States)

    Mei, Yide; Xie, Chongwei; Xie, Wei; Tian, Xu; Li, Mei; Wu, Mian

    2007-10-01

    Although camptothecin (CPT) has been reported to induce apoptosis in various cancer cells, the molecular details of this regulation remain largely unknown. In this study, we demonstrate that BH3-only protein Noxa is upregulated during CPT-induced apoptosis, which is independent of p53. In addition, we show that phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway is responsible for Noxa's induction. Luciferase assay and cAMP response element binding protein (CREB) knockdown experiments further demonstrate that CREB is involved in the transcriptional upregulation of Noxa. Moreover, blocking Noxa expression using specific small interfering ribonucleic acid (siRNA) significantly reduces the apoptosis in response to CPT, indicating that Noxa is an essential mediator for CPT-induced apoptosis. Interestingly, antiapoptotic Mcl-1 was also upregulated through PI3K/Akt signaling pathway upon CPT treatment. Using immunoprecipitation assay, Noxa was found to interact with Mcl-1 in the presence or absence of CPT. Knockdown of Mcl-1 expression by short hairpin ribonucleic acid (shRNA) was shown to potentiate CPT-induced apoptosis. Consistently, ectopic overexpression of Mcl-1 rescued cells from apoptosis induced by CPT. Cells coexpressing Noxa and Mcl-1 at different ratio correlates well with the extent of apoptosis, suggesting that the balance between Noxa and Mcl-1 may determine the susceptibility of HeLa cells to CPT-induced apoptosis.

  4. Noxa/Mcl-1 Balance Regulates Susceptibility of Cells to Camptothecin-Induced Apoptosis

    Directory of Open Access Journals (Sweden)

    Yide Mei

    2007-10-01

    Full Text Available Although camptothecin (CPT has been reported to induce apoptosis in various cancer cells, the molecular details of this regulation remain largely unknown. In this study, we demonstrate that 131-113-only protein Noxa is upregulated during CPT-induced apoptosis, which is independent of p53. In addition, we show that phosphatidylinositol 3-kinase (PI3K/Akt signaling pathway is responsible for Noxa's induction. Luciferase assay, cAMP response element binding protein (CREB knockdown experiments further demonstrate that CREB is involved in the transcriptional upregulation of Noxa. Moreover, blocking Noxa expression using specific small interfering ribonucleic acid (siRNA significantly reduces the apoptosis in response to CPT, indicating that Noxa is an essential mediator for CPT-induced apoptosis. Interestingly, antiapoptotic Mcl-1 was also upregulated through PI3K/Akt signaling pathway upon CPT treatment. Using immunoprecipitation assay, Noxa was found to interact with Mcl-1 in the presence or absence of CPT. Knockdown of Mcl-1 expression by short hairpin ribonucleic acid (shRNA was shown to potentiate CPT-induced apoptosis. Consistently, ectopic overexpression of Mcl-1 rescued cells from apoptosis induced by CPT. Cells coexpressing Noxa, Mcl-1 at different ratio correlates well with the extent of apoptosis, suggesting that the balance between Noxa, Mcl-1 may determine the susceptibility of HeLa cells to CPT-induced apoptosis.

  5. Presentation rubric: improving faculty professional presentations.

    Science.gov (United States)

    Hayne, Arlene N; McDaniel, Gretchen S

    2013-01-01

    This article describes the content of a presentation evaluation rubric for use in the development and improvement in faculty performance to enhance learning. Lectures or professional presentations require skills that can be learned through the use of evidence-based practices for all forms of public speaking. A core competency of nursing faculty is to serve as a role model in skilled oral communication. The use of an evaluation presentation rubric can increase faculty competency in this area. © 2013 Wiley Periodicals, Inc.

  6. Information-Seeking Habits of Education Faculty

    Science.gov (United States)

    Rupp-Serrano, Karen; Robbins, Sarah

    2013-01-01

    This study explores the information-seeking behavior of academic education faculty from twenty large public research universities. The investigation includes an examination of how frequently education faculty seek or access information, how they stay up-to-date on current developments in the field and identify less recent journal literature, how…

  7. Information-Seeking Habits of Education Faculty

    Science.gov (United States)

    Rupp-Serrano, Karen; Robbins, Sarah

    2013-01-01

    This study explores the information-seeking behavior of academic education faculty from twenty large public research universities. The investigation includes an examination of how frequently education faculty seek or access information, how they stay up-to-date on current developments in the field and identify less recent journal literature, how…

  8. Faculty Perceptions of Basic Skills Faculty Development

    Science.gov (United States)

    Duffy, Michelle Moreau

    2012-01-01

    This study investigated the perceptions of faculty regarding why they choose to attend basic skills faculty development; what they choose to implement in their classrooms; and how they determine the effectiveness of the strategies selected. A survey was completed by 173 full and part-time faculty from a large, suburban single-campus community…

  9. Faculty Rights to Scholarly Research

    Science.gov (United States)

    Kleinman, Molly

    2017-01-01

    This chapter provides a history of the scholarly publishing system, and explains how it has evolved to benefit corporate publishers to the detriment of faculty, universities, and the public. It offers the open access movement as a potential remedy for the publishing crisis, and the policy environment surrounding these new forms of communication.

  10. Promoting a Culture of Integrity: A Study of Faculty and Student Perceptions of Academic Dishonesty at a Large Public Midwestern University

    Science.gov (United States)

    Stevens, Tanisha N.

    2012-01-01

    Research reveals that reducing academic misconduct requires an understanding of factors that influence the two key stakeholders in the epidemic: students who engage in academically dishonest behaviors and faculty who are charged with the responsibility of reporting and deterring the behavior (e.g., Prenshaw, Straughan & Albers-Miller, 2000).…

  11. Promoting a Culture of Integrity: A Study of Faculty and Student Perceptions of Academic Dishonesty at a Large Public Midwestern University

    Science.gov (United States)

    Stevens, Tanisha N.

    2012-01-01

    Research reveals that reducing academic misconduct requires an understanding of factors that influence the two key stakeholders in the epidemic: students who engage in academically dishonest behaviors and faculty who are charged with the responsibility of reporting and deterring the behavior (e.g., Prenshaw, Straughan & Albers-Miller, 2000).…

  12. Innate immunodeficiency following genetic ablation of Mcl1 in natural killer cells.

    Science.gov (United States)

    Sathe, Priyanka; Delconte, Rebecca B; Souza-Fonseca-Guimaraes, Fernando; Seillet, Cyril; Chopin, Michael; Vandenberg, Cassandra J; Rankin, Lucille C; Mielke, Lisa A; Vikstrom, Ingela; Kolesnik, Tatiana B; Nicholson, Sandra E; Vivier, Eric; Smyth, Mark J; Nutt, Stephen L; Glaser, Stefan P; Strasser, Andreas; Belz, Gabrielle T; Carotta, Sebastian; Huntington, Nicholas D

    2014-01-01

    The cytokine IL-15 is required for natural killer (NK) cell homeostasis; however, the intrinsic mechanism governing this requirement remains unexplored. Here we identify the absolute requirement for myeloid cell leukaemia sequence-1 (Mcl1) in the sustained survival of NK cells in vivo. Mcl1 is highly expressed in NK cells and regulated by IL-15 in a dose-dependent manner via STAT5 phosphorylation and subsequent binding to the 3'-UTR of Mcl1. Specific deletion of Mcl1 in NK cells results in the absolute loss of NK cells from all tissues owing to a failure to antagonize pro-apoptotic proteins in the outer mitochondrial membrane. This NK lymphopenia results in mice succumbing to multiorgan melanoma metastases, being permissive to allogeneic transplantation and being resistant to toxic shock following polymicrobial sepsis challenge. These results clearly demonstrate a non-redundant pathway linking IL-15 to Mcl1 in the maintenance of NK cells and innate immune responses in vivo.

  13. Apoptosis induced by Ginkgo biloba (EGb761 in melanoma cells is Mcl-1-dependent.

    Directory of Open Access Journals (Sweden)

    Yufang Wang

    Full Text Available Melanoma is an aggressive skin cancer. Unfortunately, there is currently no chemotherapeutic agent available to significantly prolong the survival of the most patients with metastatic melanomas. Here we report that the Ginkgo biloba extract (EGb761, one of the most widely sold herbal supplements in the world, potently induces apoptosis in human melanoma cells by disturbing the balance between pro- and anti-apoptosis Bcl-2 family proteins. Treatment with EGb761 induced varying degrees of apoptosis in melanoma cell lines but not in melanocytes. Induction of apoptosis was caspase-dependent and appeared to be mediated by the mitochondrial pathway, in that it was associated with reduction in mitochondrial membrane potential and activation of Bax and Bak. Although EGb761 did not cause significant change in the expression levels of the BH3-only Bcl-2 family proteins Bim, Puma, Noxa, and Bad, it significantly downregulated Mcl-1 in sensitive but not resistant melanoma cells, suggesting a major role of Mcl-1 in regulating apoptosis of melanoma cells induced by EGb761. Indeed, siRNA knockdown of Mcl-1 enhanced EGb761-induced apoptosis, which was associated with increased activation of Bax and Bak. Taken together, these results demonstrate that EGb761 kills melanoma cells through the mitochondrial apoptotic pathway, and that Mcl-1 is a major regulator of sensitivity of melanoma cells to apoptosis induced by EGb761. Therefore, EGb761 with or without in combination with targeting Mcl-1 may be a useful strategy in the treatment of melanoma.

  14. A NOXA/MCL-1 Imbalance Underlies Chemoresistance of Malignant Rhabdoid Tumor Cells.

    Science.gov (United States)

    Ouchi, Kazutaka; Kuwahara, Yasumichi; Iehara, Tomoko; Miyachi, Mitsuru; Katsumi, Yoshiki; Tsuchiya, Kunihiko; Konishi, Eiichi; Yanagisawa, Akio; Hosoi, Hajime

    2016-09-01

    Malignant rhabdoid tumor (MRT) is a rare aggressive pediatric cancer characterized by inactivation of SNF5, a core subunit of SWI/SNF complexes. Previously, we showed that SNF5 contributes to transcriptional activation of NOXA, a pro-apoptotic protein that binds and inhibits the anti-apoptotic protein MCL-1. In this study, we found that NOXA expression was downregulated in MRT cell lines as well as in clinical MRT samples and that ectopically expressed NOXA bound MCL-1 and increased the sensitivity of MRT cell lines to doxorubicin (DOX) by promoting apoptosis. Consistent with this finding, knockdown of MCL-1 in MRT cell lines induced apoptosis and increased DOX sensitivity in MRT cells, and the MCL-1 inhibitor TW-37 synergized with DOX to induce MRT cell death. Our results suggest that modulation of the NOXA/MCL-1 pathway may be a potential strategy for the treatment of patients with MRT. J. Cell. Physiol. 231: 1932-1940, 2016. © 2015 Wiley Periodicals, Inc.

  15. The carboxyl-terminal tail of Noxa protein regulates the stability of Noxa and Mcl-1.

    Science.gov (United States)

    Pang, Xiaming; Zhang, Jingjing; Lopez, Hernando; Wang, Yushu; Li, Wenyang; O'Neill, Katelyn L; Evans, Jacquelynn J D; George, Nicholas M; Long, Jianhong; Chen, Yi; Luo, Xu

    2014-06-20

    The BH3-only protein Noxa is a critical mediator of apoptosis and functions primarily by sequestering/inactivating the antiapoptotic Bcl-2 family protein Mcl-1. Although Noxa is a highly labile protein, recent studies suggested that it is degraded by the proteasome in a ubiquitylation-independent manner. In the present study, we investigated the mechanism of Noxa degradation and its ability to regulate the stability of Mcl-1. We found that the ubiquitylation-independent degradation of Noxa does not require a physical association with Mcl-1. A short stretch of amino acid residues in the C-terminal tail was found to mediate the proteasome-dependent degradation of Noxa. Ectopic placement of this degron was able to render other proteins unstable. Surprisingly, mutation of this sequence not only attenuated the rapid degradation of Noxa, but also stabilized endogenous Mcl-1 through the BH3-mediated direct interaction. Together, these results suggest that the C-terminal tail of Noxa regulates the stability of both Noxa and Mcl-1.

  16. Research Productivity of Sports Medicine Fellowship Faculty

    Science.gov (United States)

    Cvetanovich, Gregory L.; Saltzman, Bryan M.; Chalmers, Peter N.; Frank, Rachel M.; Cole, Brian J.; Bach, Bernard R.

    2016-01-01

    Background: Research productivity is considered an important factor in academic advancement in sports medicine. No study to date has evaluated academic productivity and correlates of academic rank for sports medicine fellowship faculty. Purpose: To describe the academic productivity of American Orthopaedic Society for Sports Medicine (AOSSM) fellowship program faculty and to determine the association between academic productivity, fellowship characteristics, and academic rank. Study Design: Descriptive epidemiology study. Methods: Characteristics of orthopaedic sports medicine fellowship programs were obtained from the AOSSM and program websites. Metrics of academic productivity (Hirsch index [h index], I-10 index, publications, citations, and number of publications in several journals) were obtained from Scopus. Statistical analyses were conducted to determine whether academic productivity differs with fellowship attributes and academic rank. Results: A total of 90 AOSSM sports medicine fellowship programs with 610 associated faculty members were identified. Faculty were predominantly male (94%), at academic medical centers (74%), members of AOSSM (71%), and sports medicine–fellowship trained (84%). Faculty had a median of 18 (range, 0-684) publications overall, including a median of 3 (range, 0-161) publications since 2012. All measures of academic productivity were significantly higher among faculty employed at academic medical centers compared with those not employed at academic centers (P Research productivity was higher among faculty employed at academic centers in the Northeast and Midwest regions and at programs with a larger number of fellows. PMID:28210650

  17. Fragment-based discovery of potent inhibitors of the anti-apoptotic MCL-1 protein.

    Science.gov (United States)

    Petros, Andrew M; Swann, Steven L; Song, Danying; Swinger, Kerren; Park, Chang; Zhang, Haichao; Wendt, Michael D; Kunzer, Aaron R; Souers, Andrew J; Sun, Chaohong

    2014-03-15

    Apoptosis is regulated by the BCL-2 family of proteins, which is comprised of both pro-death and pro-survival members. Evasion of apoptosis is a hallmark of malignant cells. One way in which cancer cells achieve this evasion is thru overexpression of the pro-survival members of the BCL-2 family. Overexpression of MCL-1, a pro-survival protein, has been shown to be a resistance factor for Navitoclax, a potent inhibitor of BCL-2 and BCL-XL. Here we describe the use of fragment screening methods and structural biology to drive the discovery of novel MCL-1 inhibitors from two distinct structural classes. Specifically, cores derived from a biphenyl sulfonamide and salicylic acid were uncovered in an NMR-based fragment screen and elaborated using high throughput analog synthesis. This culminated in the discovery of selective and potent inhibitors of MCL-1 that may serve as promising leads for medicinal chemistry optimization efforts.

  18. Application of clustering methods: Regularized Markov clustering (R-MCL) for analyzing dengue virus similarity

    Science.gov (United States)

    Lestari, D.; Raharjo, D.; Bustamam, A.; Abdillah, B.; Widhianto, W.

    2017-07-01

    Dengue virus consists of 10 different constituent proteins and are classified into 4 major serotypes (DEN 1 - DEN 4). This study was designed to perform clustering against 30 protein sequences of dengue virus taken from Virus Pathogen Database and Analysis Resource (VIPR) using Regularized Markov Clustering (R-MCL) algorithm and then we analyze the result. By using Python program 3.4, R-MCL algorithm produces 8 clusters with more than one centroid in several clusters. The number of centroid shows the density level of interaction. Protein interactions that are connected in a tissue, form a complex protein that serves as a specific biological process unit. The analysis of result shows the R-MCL clustering produces clusters of dengue virus family based on the similarity role of their constituent protein, regardless of serotypes.

  19. Down-regulation of Mcl-1 by small interference RNA induces apoptosis and sensitizes HL-60 leukemia cells to etoposide.

    Science.gov (United States)

    Karami, Hadi; Baradaran, Behzad; Esfehani, Ali; Sakhinia, Masoud; Sakhinia, Ebrahim

    2014-01-01

    Acute myeloid leukemia (AML) is a fatal hematological malignancy which is resistant to a variety of chemotherapy drugs. Myeloid cell leukemia-1 (Mcl-1), a death-inhibiting protein that regulates apoptosis, has been shown to be overexpressed in numerous malignancies. In addition, it has been demonstrated that the expression level of the Mcl-1 gene increases at the time of leukemic relapse following chemotherapy. The aim of this study was to target Mcl-1 by small interference RNA (siRNA) and analyze its effects on survival and chemosensitivity of acute myeloid leukemia cell line HL-60. siRNA transfection was performed with a liposome approach. The expression levels of mRNA and protein were measured by real-time quantitative PCR and Western blot analysis, respectively. Trypan blue assays were performed to evaluate tumor cell growth after siRNA transfection. The cytotoxic effects of Mcl-1 siRNA (siMcl-1) and etoposide were determined using MTT assay on their own and in combination. Apoptosis was quantified using a DNA-histone ELISA assay. Transfection with siMcl-1 significantly suppressed the expression of Mcl-1 mRNA and protein in a time- dependent manner, resulting in strong growth inhibition and spontaneous apoptosis. Surprisingly, pretreatment with siMcl-1 synergistically enhanced the cytotoxic effect of etoposide. Furthermore, Mcl-1 down-regulation significantly increased apoptosis sensitivity to etoposide. No significant biological effects were observed with negative control siRNA treatment. Our results suggest that specific suppression of Mcl-1 by siRNA can effectively induce apoptosis and overcome chemoresistance of leukemic cells. Therefore, siMcl-1 may be a potent adjuvant in leukemia chemotherapy.

  20. Refined diagnostic criteria and classification of mast cell leukemia (MCL) and myelomastocytic leukemia (MML): a consensus proposal.

    Science.gov (United States)

    Valent, P; Sotlar, K; Sperr, W R; Escribano, L; Yavuz, S; Reiter, A; George, T I; Kluin-Nelemans, H C; Hermine, O; Butterfield, J H; Hägglund, H; Ustun, C; Hornick, J L; Triggiani, M; Radia, D; Akin, C; Hartmann, K; Gotlib, J; Schwartz, L B; Verstovsek, S; Orfao, A; Metcalfe, D D; Arock, M; Horny, H-P

    2014-09-01

    Mast cell leukemia (MCL), the leukemic manifestation of systemic mastocytosis (SM), is characterized by leukemic expansion of immature mast cells (MCs) in the bone marrow (BM) and other internal organs; and a poor prognosis. In a subset of patients, circulating MCs are detectable. A major differential diagnosis to MCL is myelomastocytic leukemia (MML). Although criteria for both MCL and MML have been published, several questions remain concerning terminologies and subvariants. To discuss open issues, the EU/US-consensus group and the European Competence Network on Mastocytosis (ECNM) launched a series of meetings and workshops in 2011-2013. Resulting discussions and outcomes are provided in this article. The group recommends that MML be recognized as a distinct condition defined by mastocytic differentiation in advanced myeloid neoplasms without evidence of SM. The group also proposes that MCL be divided into acute MCL and chronic MCL, based on the presence or absence of C-Findings. In addition, a primary (de novo) form of MCL should be separated from secondary MCL that typically develops in the presence of a known antecedent MC neoplasm, usually aggressive SM (ASM) or MC sarcoma. For MCL, an imminent prephase is also proposed. This prephase represents ASM with rapid progression and 5%-19% MCs in BM smears, which is generally accepted to be of prognostic significance. We recommend that this condition be termed ASM in transformation to MCL (ASM-t). The refined classification of MCL fits within and extends the current WHO classification; and should improve prognostication and patient selection in practice as well as in clinical trials.

  1. Bone marrow microenvironment-derived signals induce Mcl-1 dependence in multiple myeloma.

    Science.gov (United States)

    Gupta, Vikas A; Matulis, Shannon M; Conage-Pough, Jason E; Nooka, Ajay K; Kaufman, Jonathan L; Lonial, Sagar; Boise, Lawrence H

    2017-04-06

    Multiple myeloma is highly dependent on the bone marrow microenvironment until progressing to very advanced extramedullary stages of the disease such as plasma cell leukemia. Stromal cells in the bone marrow secrete a variety of cytokines that promote plasma cell survival by regulating antiapoptotic members of the Bcl-2 family including Mcl-1, Bcl-xL, and Bcl-2. Although the antiapoptotic protein on which a cell depends is typically consistent among normal cells of a particular phenotype, Bcl-2 family dependence is highly heterogeneous in multiple myeloma. Although normal plasma cells and most multiple myeloma cells require Mcl-1 for survival, a subset of myeloma is codependent on Bcl-2 and/or Bcl-xL We investigated the role of the bone marrow microenvironment in determining Bcl-2 family dependence in multiple myeloma. We used the Bcl-2/Bcl-xL inhibitor ABT-737 to study the factors regulating whether myeloma is Mcl-1 dependent, and thus resistant to ABT-737-induced apoptosis, or Bcl-2/Bcl-xL codependent, and thus sensitive to ABT-737. We demonstrate that bone marrow stroma is capable of inducing Mcl-1 dependence through the production of the plasma cell survival cytokine interleukin-6 (IL-6). IL-6 upregulates Mcl-1 transcription in a STAT3-dependent manner, although this occurred in a minority of the cells tested. In all cells, IL-6 treatment results in posttranslational modification of the proapoptotic protein Bim. Phosphorylation of Bim shifts its binding from Bcl-2 and Bcl-xL to Mcl-1, an effect reversed by MEK inhibition. Blocking IL-6 or downstream signaling restored Bcl-2/Bcl-xL dependence and may therefore represent a clinically useful strategy to enhance the activity of Bcl-2 inhibitors. © 2017 by The American Society of Hematology.

  2. Regulation of Mcl-1 Expression in Context to Bone Marrow Stromal Microenvironment in Chronic Lymphocytic Leukemia

    Directory of Open Access Journals (Sweden)

    Kumudha Balakrishnan, PhD

    2014-12-01

    Full Text Available A growing body of evidence suggests that the resistance of CLL cells to apoptosis is partly mediated through the interactions between leukemia cells and adjacent stromal cells residing in the lymphatic tissue or bone marrow microenvironment. Mcl-1, an anti-apoptotic protein that is associated with failure to treatment is up-regulated in CLL lymphocytes after interaction with microenvironment. However, the regulation of its expression in context to microenvironment is unclear. We evaluated and compared changes in Mcl-1 in CLL B-cells in suspension culture and when co-cultured on stromal cells. The blockade of apoptosis in co-cultured CLL cells is associated with diminution in caspase-3 and PARP cleavage and is not dependent on cytogenetic profile or prognostic factors of the disease. Stroma-derived resistance to apoptosis is associated with a cascade of transcriptional events such as increase in levels of total RNA Pol II and its phosphorylation at Ser2 and Ser5, increase in the rate of global RNA synthesis, and amplification of Mcl-1 transcript levels. The latter is associated with increase in Mcl-1 protein level without an impact on the levels of Bcl-2 and Bcl-xL. Post-translational modifications of protein kinases show increased phosphorylation of Akt at Ser473, Erk at Thr202/Tyr204 and Gsk-3β at Ser9 and augmentation of total Mcl-1 accumulation along with phosphorylation at Ser159/Thr163 sites. Collectively, stroma-induced apoptosis resistance is mediated through signaling proteins that regulate transcriptional and translational expression and post-translational modification of Mcl-1 in CLL cells in context to bone marrow stromal microenvironment.

  3. Regulation of Mcl-1 expression in context to bone marrow stromal microenvironment in chronic lymphocytic leukemia.

    Science.gov (United States)

    Balakrishnan, Kumudha; Burger, Jan A; Fu, Min; Doifode, Tejaswini; Wierda, William G; Gandhi, Varsha

    2014-12-01

    A growing body of evidence suggests that the resistance of CLL cells to apoptosis is partly mediated through the interactions between leukemia cells and adjacent stromal cells residing in the lymphatic tissue or bone marrow microenvironment. Mcl-1, an anti-apoptotic protein that is associated with failure to treatment is up-regulated in CLL lymphocytes after interaction with microenvironment. However, the regulation of its expression in context to microenvironment is unclear. We evaluated and compared changes in Mcl-1 in CLL B-cells in suspension culture and when co-cultured on stromal cells. The blockade of apoptosis in co-cultured CLL cells is associated with diminution in caspase-3 and PARP cleavage and is not dependent on cytogenetic profile or prognostic factors of the disease. Stroma-derived resistance to apoptosis is associated with a cascade of transcriptional events such as increase in levels of total RNA Pol II and its phosphorylation at Ser2 and Ser5, increase in the rate of global RNA synthesis, and amplification of Mcl-1 transcript levels. The latter is associated with increase in Mcl-1 protein level without an impact on the levels of Bcl-2 and Bcl-xL. Post-translational modifications of protein kinases show increased phosphorylation of Akt at Ser473, Erk at Thr202/Tyr204 and Gsk-3β at Ser9 and augmentation of total Mcl-1 accumulation along with phosphorylation at Ser159/Thr163 sites. Collectively, stroma-induced apoptosis resistance is mediated through signaling proteins that regulate transcriptional and translational expression and post-translational modification of Mcl-1 in CLL cells in context to bone marrow stromal microenvironment. Copyright © 2014 Neoplasia Press, Inc. Published by Elsevier Inc. All rights reserved.

  4. Alcohol-Mediated Missplicing of Mcl-1 Pre-mRNA is Involved in Neurotoxicity.

    Science.gov (United States)

    Sariyer, Rahsan; De-Simone, Francesca I; Donadoni, Martina; Hoek, Jan B; Chang, Sulie L; Sariyer, Ilker Kudret

    2017-08-11

    Heavy and chronic ethanol (EtOH) exposure can cause significant structural and functional damage to the adult brain. The most devastating consequence of EtOH exposure is the neurotoxicity associated with the depletion of neurons. Regulation of splice variants in the brain can modulate protein functions, which may ultimately affect behaviors associated with alcohol dependence and EtOH-mediated neurotoxicity. As alcohol consumption is associated with neurotoxicity, it is possible that altered splicing of survival and pro-survival factors during the development of alcoholism may contribute to the neurotoxicity. Primary human neurons and a neuroblastoma cell line were exposed to different concentrations of EtOH for various time periods. Cell viability and neuronal marker expression were analyzed by MTT assay and immunoblotting, respectively. Effect of EtOH exposure on splicing regulatory protein expression and alternative splicing of candidate genes was analyzed by a biochemical approach. Transcriptional activity of serine/arginine-rich splicing factor 1 (SRSF1) gene was determined by reporter gene analysis. Our results suggest that EtOH exposure to neuronal cells at 25 mM and higher concentrations are detrimental. In addition, EtOH exposure caused a dramatic reduction in SRSF1 expression levels. Furthermore, EtOH exposure led to pre-mRNA missplicing of Mcl-1, a pro-survival member of the Bcl-2 family, by down-regulating the expression levels of SRSF1. Moreover, ectopic expression of both SRSF1 and Mcl-1L isoform was able to recover EtOH-mediated neurotoxicity. Our results suggest that EtOH exposure can lead to pre-mRNA missplicing of Mcl-1 in neuronal cells. Our results indicate that EtOH exposure of neurons leads to a decrease in the ratio of Mcl-1L/Mcl-1S by favoring pro-apoptotic Mcl-1S splicing over anti-apoptotic Mcl-1L isoform suggesting that Mcl-1S may play a crucial role in neurotoxicity associated with alcohol consumption. Copyright © 2017 by the Research

  5. From Aardvark to Zebra: A New Millennium Analysis of Theory Development in Public Relations Academic Journals. A Top Faculty/Student Research Paper.

    Science.gov (United States)

    Sallot, Lynne M.; Lyon, Lisa J.; Acosta-Alzuru, Carolina; Jones, Karyn Ogata

    In a replication and extension of a 1984 study by M. A. Ferguson to investigate the status of theory building by public relations scholars, 748 abstracts and/or articles published in "Public Relations Review,""Journal of Public Relations Research," and its predecessor "Public Relations Research Annual," since their inceptions through the year…

  6. Successful Faculty Evaluation Programs. A Practical Guide to Improve Faculty Performance and Promotion/Tenure Decisions.

    Science.gov (United States)

    Seldin, Peter

    Seven separate areas of faculty evaluation are discussed: student, colleague and self-assessment, student learning, student advising, institutional service, and research and publication. Designed for use by faculty and administrators, this book is intended to serve as a practical resource in developing and upgrading programs for evaluation. Each…

  7. Trust or Consequences: The Relationship between Faculty Trust and Faculty Learning Communities in Higher Education

    Science.gov (United States)

    Wilson, Gaye R.

    2011-01-01

    The purpose of this study was to investigate relationships between FLC membership and faculty trust in higher education colleagues and faculty trust in higher education administration in public and private universities in the United States. This quantitative study examines trust in colleagues and trust in administration in higher education, two…

  8. Discovery of Potent Myeloid Cell Leukemia 1 (Mcl-1) Inhibitors Using Fragment-Based Methods and Structure-Based Design

    Energy Technology Data Exchange (ETDEWEB)

    Friberg, Anders [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Vigil, Dominico [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Zhao, Bin [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Daniels, R. Nathan [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Burke, Jason P. [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Garcia-Barrantes, Pedro M. [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Camper, DeMarco [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Chauder, Brian A. [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Lee, Taekyu [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Olejniczak, Edward T. [Vanderbilt Univ. School of Medicine, Nashville, TN (United States); Fesik, Stephen W. [Vanderbilt Univ. School of Medicine, Nashville, TN (United States)

    2012-12-17

    Myeloid cell leukemia 1 (Mcl-1), a member of the Bcl-2 family of proteins, is overexpressed and amplified in various cancers and promotes the aberrant survival of tumor cells that otherwise would undergo apoptosis. Here we describe the discovery of potent and selective Mcl-1 inhibitors using fragment-based methods and structure-based design. NMR-based screening of a large fragment library identified two chemically distinct hit series that bind to different sites on Mcl-1. Members of the two fragment classes were merged together to produce lead compounds that bind to Mcl-1 with a dissociation constant of <100 nM with selectivity for Mcl-1 over Bcl-xL and Bcl-2. Structures of merged compounds when complexed to Mcl-1 were obtained by X-ray crystallography and provide detailed information about the molecular recognition of small-molecule ligands binding Mcl-1. The compounds represent starting points for the discovery of clinically useful Mcl-1 inhibitors for the treatment of a wide variety of cancers.

  9. Phenylethyl isothiocyanate reverses cisplatin resistance in biliary tract cancer cells via glutathionylation-dependent degradation of Mcl-1.

    Science.gov (United States)

    Li, Qiwei; Zhan, Ming; Chen, Wei; Zhao, Benpeng; Yang, Kai; Yang, Jie; Yi, Jing; Huang, Qihong; Mohan, Man; Hou, Zhaoyuan; Wang, Jian

    2016-03-01

    Biliary tract cancer (BTC) is a highly malignant cancer. BTC exhibits a low response rate to cisplatin (CDDP) treatment, and therefore, an understanding of the mechanism of CDDP resistance is urgently needed. Here, we show that BTC cells develop CDDP resistance due, in part, to upregulation of myeloid cell leukemia 1 (Mcl-1). Phenylethyl isothiocyanate (PEITC), a natural compound found in watercress, could enhance the efficacy of CDDP by degrading Mcl-1. PEITC-CDDP co-treatment also increased the rate of apoptosis of cancer stem-like side population (SP) cells and inhibited xenograft tumor growth without obvious toxic effects. In vitro, PEITC decreased reduced glutathione (GSH), which resulted in decreased GSH/oxidized glutathione (GSSG) ratio and increased glutathionylation of Mcl-1, leading to rapid proteasomal degradation of Mcl-1. Furthermore, we identified Cys16 and Cys286 as Mcl-1 glutathionylation sites, and mutating them resulted in PEITC-mediated degradation resistant Mcl-1 protein. In conclusion, we demonstrate for the first time that CDDP resistance is partially associated with Mcl-1 in BTC cells and we identify a novel mechanism that PEITC can enhance CDDP-induced apoptosis via glutathionylation-dependent degradation of Mcl-1. Hence, our results provide support that dietary intake of watercress may help reverse CDDP resistance in BTC patients.

  10. 40 CFR 141.209 - Special notice for nitrate exceedances above MCL by non-community water systems (NCWS), where...

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Special notice for nitrate exceedances... Water Violations § 141.209 Special notice for nitrate exceedances above MCL by non-community water... primacy agency under § 141.11(d) to exceed the nitrate MCL must provide notice to persons served...

  11. Lo que Piensan los Estudiantes y Profesores Sobre la Calidad de la Educacion Superior. Estudio Comparativo en 5 Instituciones de Educacion Superior--dos publicas y tres privadas--en Guadalajara, Jalisco, Mexico (What Students and Faculties Think about the Quality of Higher Education. Comparative Study of 5 Higher Education Institutions--Two Public and Three Private--in Guadalajara, Jalisco, Mexico).

    Science.gov (United States)

    Yanez, Maria Lorena Hernandez

    This study, written in Spanish, compared attitudes of students (N=302) and faculty (N=28) at five institutions of higher education (two public and three private) in Guadalajara, Jalisco, Mexico. The study explored first, whether respondents believed there are significant quality differences between private and public universities and, second, what…

  12. miR-107 activates ATR/Chk1 pathway and suppress cervical cancer invasion by targeting MCL1.

    Directory of Open Access Journals (Sweden)

    Chengyan Zhou

    Full Text Available MicroRNAs (miRNAs are a class of single-stranded, non-coding RNAs of about 22 nucleotides in length. Increasing evidence implicates miRNAs may function as oncogenes or tumor suppressors. Here we showed that miR-107 directly targeted MCL1 and activated ATR/Chk1 pathway to inhibit proliferation, migration and invasiveness of cervical cancer cells. Moreover, we found that MCL1 was frequently up-regulated in cervical cancer, and knockdown of MCL1 markedly inhibited cancer cell proliferation, migration and invasion, whereas ectopic expression of MCL1 significantly enhances these properties. The restoration of MCL1 expression can counteract the effect of miR-107 on the cancer cells. Together, miR-107 is a new regulator of MCL1, and both miR-107 and MCL1 play important roles in the pathogenesis of cervical cancer. We have therefore identified a mechanism for ATR/Chk1 pathway which involves an increase in miR-107 leading to a decrease in MCL1. Correspondingly, our results revealed that miR-107 affected ATR/Chk1 signalling and gene expression, and implicated miR-107 as a therapeutic target in human cervical cancer. We also demonstrated that taxol attenuated migration and invasion in cervical cancer cells by activating the miR-107, in which miR-107 play an important role in regulating the expression of MCL1. Elucidation of this discovered MCL1 was directly regulated by miR-107 will greatly enhance our understanding of the mechanisms responsible for cervical cancer and will provide an additional arm for the development of anticancer therapies.

  13. The downregulation of Mcl-1 via USP9X inhibition sensitizes solid tumors to Bcl-xl inhibition

    Directory of Open Access Journals (Sweden)

    Peddaboina Chander

    2012-11-01

    Full Text Available Abstract Background It has been shown in many solid tumors that the overexpression of the pro-survival Bcl-2 family members Bcl-xL and Mcl-1 confers resistance to a variety of chemotherapeutic agents. Mcl-1 is a critical survival protein in a variety of cell lineages and is critically regulated via ubiquitination. Methods The Mcl-1, Bcl-xL and USP9X expression patterns in human lung and colon adenocarcinomas were evaluated via immunohistochemistry. Interaction between USP9X and Mcl-1 was demonstrated by immunoprecipitation-western blotting. The protein expression profiles of Mcl-1, Bcl-xL and USP9X in multiple cancer cell lines were determined by western blotting. Annexin-V staining and cleaved PARP western blotting were used to assay for apoptosis. The cellular toxicities after various treatments were measured via the XTT assay. Results In our current analysis of colon and lung cancer samples, we demonstrate that Mcl-1 and Bcl-xL are overexpressed and also co-exist in many tumors and that the expression levels of both genes correlate with the clinical staging. The downregulation of Mcl-1 or Bcl-xL via RNAi was found to increase the sensitivity of the tumor cells to chemotherapy. Furthermore, our analyses revealed that USP9X expression correlates with that of Mcl-1 in human cancer tissue samples. We additionally found that the USP9X inhibitor WP1130 promotes Mcl-1 degradation and increases tumor cell sensitivity to chemotherapies. Moreover, the combination of WP1130 and ABT-737, a well-documented Bcl-xL inhibitor, demonstrated a chemotherapeutic synergy and promoted apoptosis in different tumor cells. Conclusion Mcl-1, Bcl-xL and USP9X overexpression are tumor survival mechanisms protective against chemotherapy. USP9X inhibition increases tumor cell sensitivity to various chemotherapeutic agents including Bcl-2/Bcl-xL inhibitors.

  14. Predictors of turnover intention in nurse faculty.

    Science.gov (United States)

    Gormley, Denise K; Kennerly, Susan

    2011-04-01

    Turnover of nurse faculty is an increasingly important issue in nursing as the available number of qualified faculty continues to decrease. Understanding the factors that contribute to turnover is important to academic administrators to retain and recruit qualified nursing faculty. The purpose of this study was to examine predictors of turnover intention in nurse faculty working in departments and schools of nursing in Carnegie Doctoral/Research Universities-Extensive, public and private, not-for-profit institutions. The multidimensional model of organizational commitment was used to frame this study. The predictor variables explored were organizational climate, organizational commitment, work role balance, role ambiguity, and role conflict. The work roles examined were research, teaching, and service. Logistical regression was performed to examine the predictors of turnover intention. Organizational climate intimacy and disengagement, affective and continuance organizational commitment, and role ambiguity were shown to predict turnover intention in nurse faculty. Copyright 2011, SLACK Incorporated.

  15. Rapid Optimization of Mcl-1 Inhibitors using Stapled Peptide Libraries Including Non-Natural Side Chains.

    Science.gov (United States)

    Rezaei Araghi, Raheleh; Ryan, Jeremy A; Letai, Anthony; Keating, Amy E

    2016-05-20

    Alpha helices form a critical part of the binding interface for many protein-protein interactions, and chemically stabilized synthetic helical peptides can be effective inhibitors of such helix-mediated complexes. In particular, hydrocarbon stapling of peptides to generate constrained helices can improve binding affinity and other peptide properties, but determining the best stapled peptide variant often requires laborious trial and error. Here, we describe the rapid discovery and optimization of a stapled-helix peptide that binds to Mcl-1, an antiapoptotic protein that is overexpressed in many chemoresistant cancers. To accelerate discovery, we developed a peptide library synthesis and screening scheme capable of identifying subtle affinity differences among Mcl-1-binding stapled peptides. We used our method to sample combinations of non-natural amino-acid substitutions that we introduced into Mcl-1 inhibitors in the context of a fixed helix-stabilizing hydrocarbon staple that increased peptide helical content and reduced proteolysis. Peptides discovered in our screen contained surprising substitutions at sites that are conserved in natural binding partners. Library-identified peptide M3d is the most potent molecule yet tested for selectively triggering mitochondrial permeabilization in Mcl-1 dependent cell lines. Our library approach for optimizing helical peptide inhibitors can be readily applied to the study of other biomedically important targets.

  16. A Full-Time Dilemma: Examining the Experiences of Part-Time Faculty

    Science.gov (United States)

    Kimmel, Krista M.; Fairchild, Jennifer L.

    2017-01-01

    Part-time faculty now account for more than half of all faculty in American colleges and universities. Existing scholarship primarily has focused on the teaching effectiveness of part-time faculty. In this exploratory study, the authors employ a qualitative approach to examine the perspectives of part-time faculty members at a public, regional…

  17. Faculty Compensation Systems: Impact on the Quality of Higher Education. ERIC Digest.

    Science.gov (United States)

    Sutton, Terry P.; Bergerson, Peter J.

    Faculty compensation is a critical management tool for increasing faculty productivity, improving cost efficiency, and enhancing an institution's public image. Factors that determine faculty compensation include academic rank, faculty productivity, discipline market pay, ability to obtain external grants, seniority or length of service, service in…

  18. A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells

    DEFF Research Database (Denmark)

    Thrane, S; Pedersen, A M; Thomsen, M B H;

    2015-01-01

    of the anti-apoptotic protein Mcl-1. Mcl-1 expression was found upregulated in the antiestrogen-resistant cell lines and depletion of Mcl-1 in resistant cells caused decreased viability. JNJ-7706621, a dual Aurora kinase and cyclin-dependent kinase inhibitor, specifically inhibited growth and caused G2 phase...

  19. Circumvention of Mcl-1-Dependent Drug Resistance by Simultaneous Chk1 and MEK1/2 Inhibition in Human Multiple Myeloma Cells

    Science.gov (United States)

    Pei, Xin-Yan; Dai, Yun; Felthousen, Jessica; Chen, Shuang; Takabatake, Yukie; Zhou, Liang; Youssefian, Leena E.; Sanderson, Michael W.; Bodie, Wesley W.; Kramer, Lora B.; Orlowski, Robert Z.; Grant, Steven

    2014-01-01

    The anti-apoptotic protein Mcl-1 plays a major role in multiple myeloma (MM) cell survival as well as bortezomib- and microenvironmental forms of drug resistance in this disease. Consequently, there is a critical need for strategies capable of targeting Mcl-1-dependent drug resistance in MM. The present results indicate that a regimen combining Chk1 with MEK1/2 inhibitors effectively kills cells displaying multiple forms of drug resistance stemming from Mcl-1 up-regulation in association with direct transcriptional Mcl-1 down-regulation and indirect disabling of Mcl-1 anti-apoptotic function through Bim up-regulation and increased Bim/Mcl-1 binding. These actions release Bak from Mcl-1, accompanied by Bak/Bax activation. Analogous events were observed in both drug-naïve and acquired bortezomib-resistant MM cells displaying increased Mcl-1 but diminished Bim expression, or cells ectopically expressing Mcl-1. Moreover, concomitant Chk1 and MEK1/2 inhibition blocked Mcl-1 up-regulation induced by IL-6/IGF-1 or co-culture with stromal cells, effectively overcoming microenvironment-related drug resistance. Finally, this regimen down-regulated Mcl-1 and robustly killed primary CD138+ MM cells, but not normal hematopoietic cells. Together, these findings provide novel evidence that this targeted combination strategy could be effective in the setting of multiple forms of Mcl-1-related drug resistance in MM. PMID:24594907

  20. Circumvention of Mcl-1-dependent drug resistance by simultaneous Chk1 and MEK1/2 inhibition in human multiple myeloma cells.

    Directory of Open Access Journals (Sweden)

    Xin-Yan Pei

    Full Text Available The anti-apoptotic protein Mcl-1 plays a major role in multiple myeloma (MM cell survival as well as bortezomib- and microenvironmental forms of drug resistance in this disease. Consequently, there is a critical need for strategies capable of targeting Mcl-1-dependent drug resistance in MM. The present results indicate that a regimen combining Chk1 with MEK1/2 inhibitors effectively kills cells displaying multiple forms of drug resistance stemming from Mcl-1 up-regulation in association with direct transcriptional Mcl-1 down-regulation and indirect disabling of Mcl-1 anti-apoptotic function through Bim up-regulation and increased Bim/Mcl-1 binding. These actions release Bak from Mcl-1, accompanied by Bak/Bax activation. Analogous events were observed in both drug-naïve and acquired bortezomib-resistant MM cells displaying increased Mcl-1 but diminished Bim expression, or cells ectopically expressing Mcl-1. Moreover, concomitant Chk1 and MEK1/2 inhibition blocked Mcl-1 up-regulation induced by IL-6/IGF-1 or co-culture with stromal cells, effectively overcoming microenvironment-related drug resistance. Finally, this regimen down-regulated Mcl-1 and robustly killed primary CD138+ MM cells, but not normal hematopoietic cells. Together, these findings provide novel evidence that this targeted combination strategy could be effective in the setting of multiple forms of Mcl-1-related drug resistance in MM.

  1. Silence of MCL-1 upstream signaling by shRNA abrogates multiple myeloma growth.

    Science.gov (United States)

    Wang, Mengchang; Wu, Di; Liu, Pengbo; Deng, Jiusheng

    2014-01-01

    Multiple myeloma (MM) is an incurable B-cell cancer with accumulated clonal abnormal plasma cells in bone marrow of patients. MCL-1 (myeloid cell leukemia sequence 1) protein is an anti-apoptotic molecule in MM cells and regulated by pro-inflammatory cytokine IL-6 and downstream signaling molecules STAT3, PI3K and MAPK. The purpose of this study is to investigate the effect of STAT3, PI3K and MAPK gene silence on MCL-1 expression in human MM cells and the consequence of cell survival. Lentivirus small hairpin RNA (shRNA) interference techniques were utilized to knock down STAT3, PI3K or MAPK genes. Gene and protein expression was quantified by quantitative real-time PCR and Western Blot. MM cell apoptosis was examined by annexin-V FITC/propidium iodide staining. Efficient silence of STAT3, PI3K, MAPK1 or MAPK2 gene robustly abrogated IL-6 enhanced MCL-1 expression and suppressed MM cell growth. Silencing STAT3 gene inhibited PI3K expression, silencing PI3K markedly abrogated STAT3 and MAPK production. Inhibition of MAPK2 gene by shMAPK2 suppressed STAT3, PI3K and MAPK1 expression in the cells. Silencing of STAT3, PI3K and MAPK2 together completely blocked MCL-1 expression in MM cells. There is a syngeneic effect among the three independent STAT3, PI3K and MAPK2 survival-signaling pathways related to MCL-1 expression in MM cells. shRNAs silencing of STAT3, PI3K and MAPK2 together could provide an effective strategy to treat MM.

  2. Nursing Faculty Members' Perspectives of Faculty-to-Faculty Workplace Incivility among Nursing Faculty Members

    Science.gov (United States)

    Amos, Kimberly S.

    2013-01-01

    In recent years, nursing faculty incivility has been a searing topic of research. Nursing research included studies on incivility among nursing students, incivility between nursing students and nursing faculty, and incivility in the clinical setting. However, literature specifically on nursing faculty incivility was limited. This descriptive,…

  3. Students Evaluation of Faculty

    Science.gov (United States)

    Thawabieh, Ahmad M.

    2017-01-01

    This study aimed to investigate how students evaluate their faculty and the effect of gender, expected grade, and college on students' evaluation. The study sample consisted of 5291 students from Tafila Technical University Faculty evaluation scale was used to collect data. The results indicated that student evaluation of faculty was high (mean =…

  4. Nursing Faculty Members' Perspectives of Faculty-to-Faculty Workplace Incivility among Nursing Faculty Members

    Science.gov (United States)

    Amos, Kimberly S.

    2013-01-01

    In recent years, nursing faculty incivility has been a searing topic of research. Nursing research included studies on incivility among nursing students, incivility between nursing students and nursing faculty, and incivility in the clinical setting. However, literature specifically on nursing faculty incivility was limited. This descriptive,…

  5. Faculty ethics: ideal principles with practical applications.

    Science.gov (United States)

    Reybold, L Earle

    2009-01-01

    Ethics in higher education is the subject of intense public attention, with considerable focus on faculty roles and responsibilities. Media reports and scholarly research have documented egregious misconduct that includes plagiarism, falsification of data, illicit teacher-student relationships, and grading bias. These accounts of wrongdoing often portray faculty ethicality as only a legal issue of obeying rules and regulations, especially in the teaching and research roles. My discussion challenges this narrow perspective and argues that characterizations of faculty ethicality should take into account broader expectations for professionalism such as collegiality, respect, and freedom of inquiry. First, I review the general principles of faculty ethics developed by the American Association of University Professors, as well as professional codes of ethics in specific professional fields. Second, I juxtapose the experiences of women and minority faculty members in relation to these general codes of ethics. This section examines three issues that particularly affect women and minority faculty experiences of ethicality: "chilly and alienating" academic climates, "cultural taxation" of minority identity, and the snare of conventional reward systems. Third, I suggest practical strategies to reconcile faculty practice with codes of ethics. My challenge is to the faculty as a community of practice to engage professional ethics as social and political events, not just legal and moral failures.

  6. The VTLISFG motif in the BH1 domain plays a significant role in regulating the degradation of Mcl-1

    Directory of Open Access Journals (Sweden)

    Kang Xiao

    2014-01-01

    Full Text Available Mcl-1 is a member of the Bcl-2 family protein; its degradation is required for the initiation of apoptosis. The mechanism, however, is not yet clearly known. Previously, it was reported that Mcl-1 is degraded through the ubiquitination-mediated pathway and the PEST domain is the motif responsible for promoting this degradation. We found evidence that this may not be true. We generated several Mcl-1 deletion mutants and examined their effects on protein stability. Deletion of the PEST domain did not prevent the degradation of Mcl-1 during apoptosis. The BH1 domain, but not the PEST, BH3 or BH2 domain, exhibited a short half-life. A peptide named “F3” (VTLISFG in the C-terminus of the BH1 domain appears to be critical for the rapid turnover of Mcl-1. Deletion of F3 from GFP-Mcl-1-ΔPEST retarded the degradation of this mutant. F3 appeared to be the minimum functional sequence of the degradation motif, since deletion of a single residue was sufficient to abrogate its short half-life. Fusion of F3 with p32 resulted in the degradation of p32 during UV-induced apoptosis, while wild type p32 was not affected. Taken together, these findings suggest that F3 (VTLISFG, instead of PEST, is the major motif responsible for the degradation of Mcl-1 during apoptosis.

  7. Noxa induces apoptosis in oncogene-expressing cells through catch-and-release mechanism operating between Puma and Mcl-1.

    Science.gov (United States)

    Nakajima, Wataru; Tanaka, Nobuyuki

    2011-10-07

    Tumor suppressor p53 induces apoptosis by transcriptional induction of Noxa and Puma, which encode the proapoptotic BH3-only member of the Bcl-2 family proteins. In the p53-mediated tumor surveillance system, p53 induces apoptosis or replicative senescence in oncogene-expressing cells, resulting in elimination of such cells. In this context, we previously found that Noxa and Puma synergistically induce apoptosis. Here, we found the adenovirus oncogene E1A to induce p53-dependently expression of Puma, but not Noxa. The induced Puma associates with antiapoptotic Bcl-2 protein Mcl-1, accompanied by accumulated Mcl-1 protein on mitochondria. Moreover, E1A also reduces expression of the antiapoptotic Bcl-2 protein Bcl-X(L). In contrast, the DNA-damaging agent adriamycin induces Noxa expression in E1A-expressing cells. Interestingly, Mcl-1 knockdown itself induced apoptosis in E1A-expressing MEFs. Furthermore, Noxa displaced Puma's association with Mcl-1, accompanied by Mcl-1 degradation and apoptosis induction by activating mitochondrial apoptotic executers Bax and Bak. These results suggest that p53-induced apoptosis in oncogene-expressing cells is regulated by differential induction and sequential activation of Noxa and Puma. Accumulated Puma by oncogene enhances susceptibility to apoptosis through "catch" in mitochondria by Mcl-1. Subsequently, in response to DNA-damage, Noxa efficiently induces apoptosis by "release" of Puma from Mcl-1.

  8. The VTLISFG motif in the BH1 domain plays a significant role in regulating the degradation of Mcl-1.

    Science.gov (United States)

    Xiao, Kang; Chen, Pengxuan; Chang, Donald Choy

    2014-01-01

    Mcl-1 is a member of the Bcl-2 family protein; its degradation is required for the initiation of apoptosis. The mechanism, however, is not yet clearly known. Previously, it was reported that Mcl-1 is degraded through the ubiquitination-mediated pathway and the PEST domain is the motif responsible for promoting this degradation. We found evidence that this may not be true. We generated several Mcl-1 deletion mutants and examined their effects on protein stability. Deletion of the PEST domain did not prevent the degradation of Mcl-1 during apoptosis. The BH1 domain, but not the PEST, BH3 or BH2 domain, exhibited a short half-life. A peptide named "F3" (VTLISFG) in the C-terminus of the BH1 domain appears to be critical for the rapid turnover of Mcl-1. Deletion of F3 from GFP-Mcl-1-ΔPEST retarded the degradation of this mutant. F3 appeared to be the minimum functional sequence of the degradation motif, since deletion of a single residue was sufficient to abrogate its short half-life. Fusion of F3 with p32 resulted in the degradation of p32 during UV-induced apoptosis, while wild type p32 was not affected. Taken together, these findings suggest that F3 (VTLISFG), instead of PEST, is the major motif responsible for the degradation of Mcl-1 during apoptosis.

  9. Quercetin induces tumor-selective apoptosis through down-regulation of Mcl-1 and activation of Bax

    Science.gov (United States)

    Cheng, Senping; Gao, Ning; Zhang, Zhuo; Chen, Gang; Budhraja, Amit; Ke, Zunji; Son, Young-ok; Wang, Xin; Luo, Jia; Shi, Xianglin

    2010-01-01

    Purpose To investigate the in vivo antitumor efficacy of querctin in U937 xenografts and the functional role of Mcl-1 and Bax in quercetin-induced apoptosis in human leukemia cells. Experimental Design Leukemia cells were treated with quercetin, after which apoptosis, Mcl-1 expression, and Bax activation and translocation were evaluated. The efficacy of quercein, as well as Mcl-1 expression and Bax activation were investigated in xenografts of leukemia cells. Results Administration of quercetin caused pronounced apoptosis in both transformed and primary leukemia cells, but not in normal blood peripheral mononuclear cells. Quercetin-induced apoptosis was accompanied by Mcl-1 down-regulation and Bax conformational change and mitochondrial translocation which triggered cytochrome c release. Knockdown of Bax by siRNA reversed querctin-induced apoptosis. Knockout of Bax abrogated the activation of caspase and apoptosis. Ectopic expression of Mcl-1 attenuated quercetin-mediated Bax activation, translocation and cell death. Conversely, interruption of Mcl-1 by siRNA enhanced Bax activation and translocation, as well as lethality induced by quercetin. However, the absence of Bax had no effect on quercetin-mediated Mcl-1 down-regulation. Furthermore, in vivo administration of quercetin attenuated tumor growth in U937 xenografts. The TUNEL positive apoptotic cells in tumor sections increased in quercetin-treated mice as compared with controls. Mcl-1 down-regulation and Bax activation were observed in xenografts. Conclusions These data suggest that quercetin may be useful for the treatment of leukemia by preferentially inducing apoptosis in leukemia versus normal hematopoietic cells, through a process involving Mcl-1 down-regulation, which in turn potentiates Bax activation and mitochondrial translocation, culminating in apoptosis. PMID:21138867

  10. CDK Inhibitors Roscovitine and CR8 Trigger Mcl-1 Down-Regulation and Apoptotic Cell Death in Neuroblastoma Cells.

    Science.gov (United States)

    Bettayeb, Karima; Baunbæk, Dianne; Delehouze, Claire; Loaëc, Nadège; Hole, Alison J; Baumli, Sonja; Endicott, Jane A; Douc-Rasy, Setha; Bénard, Jean; Oumata, Nassima; Galons, Hervé; Meijer, Laurent

    2010-04-01

    Neuroblastoma (NB), the most frequent extracranial solid tumor of children accounting for nearly 15% of all childhood cancer mortality, displays overexpression of antiapoptotic Bcl-2 and Mcl-1 in aggressive forms of the disease. The clinical phase 2 drug roscovitine (CYC202, seliciclib), a relatively selective inhibitor of cyclin-dependent kinases (CDKs), and CR8, a recently developed and more potent analog, induce concentration-dependent apoptotic cell death of NB cells (average IC(50) values: 24.2 µM and 0.4 µM for roscovitine and CR8, respectively). Both roscovitine and CR8 trigger rapid down-regulation of the short-lived survival factor Mcl-1 in the 9 investigated human NB cell lines. This effect was further analyzed in the human SH-SY5Y NB cell line. Down-regulation of Mcl-1 appears to depend on inhibition of CDKs rather than on interaction of roscovitine and CR8 with their secondary targets. CR8 is an adenosine triphosphate-competitive inhibitor of CDK9, and the structure of a CDK9/cyclin T/CR8 complex is described. Mcl-1 down-regulation occurs both at the mRNA and protein levels. This effect can be accounted for by a reduction in Mcl-1 protein synthesis, under stable Mcl-1 degradation conditions. Mcl-1 down-regulation is accompanied by a transient increase in free Noxa, a proapoptotic factor. Mcl-1 down-regulation occurs independently of the presence or up-regulation of p53 and of the MYCN status. Taken together, these results suggest that the clinical drug roscovitine and its novel analog CR8 induce apoptotic tumor cell death by down-regulating Mcl-1, a key survival factor expressed in all NB cell lines. CDK inhibition may thus constitute a new approach to treat refractory high-risk NB.

  11. Small interfering RNA targeting mcl-1 enhances proteasome inhibitor-induced apoptosis in various solid malignant tumors

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    Zhou Wei

    2011-11-01

    Full Text Available Abstract Background Targeting the ubiquitin-proteasome pathway is a promising approach for anticancer strategies. Recently, we found Bik accumulation in cancer cell lines after they were treated with bortezomib. However, recent evidence indicates that proteasome inhibitors may also induce the accumulation of anti-apoptotic Bcl-2 family members. The current study was designed to analyze the levels of several anti-apoptotic members of Bcl-2 family in different human cancer cell lines after they were treated with proteasome inhibitors. Methods Different human cancer cell lines were treated with proteasome inhibitors. Western blot were used to investigate the expression of Mcl-1 and activation of mitochondrial apoptotic signaling. Cell viability was investigated using SRB assay, and induction of apoptosis was measured using flow cytometry. Results We found elevated Mcl-1 level in human colon cancer cell lines DLD1, LOVO, SW620, and HCT116; human ovarian cancer cell line SKOV3; and human lung cancer cell line H1299, but not in human breast cancer cell line MCF7 after they were treated with bortezomib. This dramatic Mcl-1 accumulation was also observed when cells were treated with other two proteasome inhibitors, MG132 and calpain inhibitor I (ALLN. Moreover, our results showed Mcl-1 accumulation was caused by stabilization of the protein against degradation. Reducing Mcl-1 accumulation by Mcl-1 siRNA reduced Mcl-1 accumulation and enhanced proteasome inhibitor-induced cell death and apoptosis, as evidenced by the increased cleavage of caspase-9, caspase-3, and poly (ADP-ribose polymerase. Conclusions Our results showed that it was not only Bik but also Mcl-1 accumulation during the treatment of proteasome inhibitors, and combining proteasome inhibitors with Mcl-1 siRNA would enhance the ultimate anticancer effect suggesting this combination might be a more effective strategy for cancer therapy.

  12. p32, a novel binding partner of Mcl-1, positively regulates mitochondrial Ca{sup 2+} uptake and apoptosis

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, Kang [Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong (China); Wang, Yinyin; Chang, Zhijie [School of Medicine, Tsinghua University, Beijing (China); Lao, Yuanzhi, E-mail: laurence_ylao@163.com [School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai (China); Chang, Donald C., E-mail: bochang@ust.hk [Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong (China)

    2014-08-22

    Highlights: • p32 binds to Mcl-1. • p32 affects apoptosis. • p32 and Mcl-1 regulate mitochondrial Ca{sup 2+}. - Abstract: Mcl-1 is a major anti-apoptotic Bcl-2 family protein. It is well known that Mcl-1 can interact with certain pro-apoptotic Bcl-2 family proteins in normal cells to neutralize their pro-apoptotic functions, thus prevent apoptosis. In addition, it was recently found that Mcl-1 can also inhibit mitochondrial calcium uptake. The detailed mechanism, however, is still not clear. Based on Yeast Two-Hybrid screening and co-immunoprecipitation, we identified a mitochondrial protein p32 (C1qbp) as a novel binding partner of Mcl-1. We found that p32 had a number of interesting properties: (1) p32 can positively regulate UV-induced apoptosis in HeLa cells. (2) Over-expressing p32 could significantly promote mitochondrial calcium uptake, while silencing p32 by siRNA suppressed it. (3) In p32 knockdown cells, Ruthenium Red treatment (an inhibitor of mitochondrial calcium uniporter) showed no further suppressive effect on mitochondrial calcium uptake. In addition, in Ruthenium Red treated cells, Mcl-1 also failed to suppress mitochondrial calcium uptake. Taken together, our findings suggest that p32 is part of the putative mitochondrial uniporter that facilitates mitochondrial calcium uptake. By binding to p32, Mcl-1 can interfere with the uniporter function, thus inhibit the mitochondrial Ca{sup 2+} uploading. This may provide a novel mechanism to explain the anti-apoptotic function of Mcl-1.

  13. Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity

    Directory of Open Access Journals (Sweden)

    Fu Freddie H

    2011-06-01

    Full Text Available Abstract Background The human anterior cruciate ligament (hACL and medial collateral ligament (hMCL of the knee joint are frequently injured, especially in athletic settings. It has been known that, while injuries to the MCL typically heal with conservative treatment, ACL injuries usually do not heal. As adult stem cells repair injured tissues through proliferation and differentiation, we hypothesized that the hACL and hMCL contain stem cells exhibiting unique properties that could be responsible for the differential healing capacity of the two ligaments. Methods To test the above hypothesis, we derived ligament stem cells from normal hACL and hMCL samples from the same adult donors using tissue culture techniques and characterized their properties using immunocytochemistry, RT-PCR, and flow cytometry. Results We found that both hACL stem cells (hACL-SCs and hMCL stem cells (hMCL-SCs formed colonies in culture and expressed stem cell markers nucleostemin and stage-specific embryonic antigen-4 (SSEA-4. Moreover, both hACL-SCs and hMCL-SCs expressed CD surface markers for mesenchymal stem cells, including CD44 and CD90, but not those markers for vascular cells, CD31, CD34, CD45, and CD146. However, hACL-SCs differed from hMCL-SCs in that the size and number of hACL-SC colonies in culture were much smaller and grew more slowly than hMCL-SC colonies. Moreover, fewer hACL-SCs in cell colonies expressed stem cell markers STRO-1 and octamer-binding transcription factor-4 (Oct-4 than hMCL-SCs. Finally, hACL-SCs had less multi-differentiation potential than hMCL-SCs, evidenced by differing extents of adipogenesis, chondrogenesis, and osteogenesis in the respective induction media. Conclusions This study shows for the first time that hACL-SCs are intrinsically different from hMCL-SCs. We suggest that the differences in their properties contribute to the known disparity in healing capabilities between the two ligaments.

  14. Research progress of MCL-1 gene%髓系白血病-1的基因研究进展

    Institute of Scientific and Technical Information of China (English)

    郭玉洁

    2011-01-01

    MCL-1 gene is a antiapoptotic gene belong to BCL-2 gene family, its product MCL-1 protein plays an important role in cell apoptosis regulating and the course of hematologic malignancies. This paper reviews advance of studies on the function of MCL-1 gene and MCL-1 protein, the role of MCL-1 protein in hematologic malignancies and the relationship between MCL-1 protein and other apoptosis adjustment factors.%抗凋亡基因髓样细胞白血病—1( MCL-1)属bcl-2基因家族中成员之一,其表达产物MCL-1蛋白在细胞凋亡调节与血液系统恶性肿瘤的发病过程中起重要作用。文章就MCL-1基因及MCL-1蛋白的特点、MCL-1蛋白在血液系统恶性肿瘤中的作用、MCL-1基因、蛋白与其他凋亡调节因子关系的研究进展进行综述。

  15. Microbiological Common Language (MCL): a standard for electronic information exchange in the Microbial Commons.

    Science.gov (United States)

    Verslyppe, Bert; Kottmann, Renzo; De Smet, Wim; De Baets, Bernard; De Vos, Paul; Dawyndt, Peter

    2010-01-01

    Although Biological Resource Centers (BRCs) traditionally have open catalogs of their holdings, it is quite cumbersome to access meta-information about microorganisms electronically due to the variety of access methods used by those catalogs. Therefore, we propose Microbiological Common Language (MCL), aimed at standardizing the electronic exchange of meta-information about microorganisms. Its application ranges from representing the online catalog of a single collection to accessing the results of StrainInfo integration and ad hoc use in other contexts. The abstract model of the standard precisely defines the elements of the standard, which enables implementation using a variety of representation technologies. Currently, XML and RDF/XML implementations are readily available. MCL is an open standard, and therefore greatly encourages input from the microbiological community.

  16. Feasibility of biochemical synthesis of biodegradable mcl-PHA from dyeing wastewater%印染废水生化合成可降解mcl-PHA的可行性

    Institute of Scientific and Technical Information of China (English)

    王志雄; 虞倩倩; 缪伟伟

    2012-01-01

    Biodegradable polymer mcl-PHA (medium-chain-length Polyhydroxyalkanoate) is produced by Pseudomonas Putida which exists in printing and dyeing effluents and combined with model agents. The monomer structure, adhesive capacity and sustained-releasing performance of PHA are tested. The experiments verify that the production of mcl-PHA with printing and dyeing effluents is possible, and the monomer structure of mcl-PHA is closely related to the type of effluents. mcl-PHA can be used as adhesive, cross-linking agent in finishing.%利用印染废水中存在的厌氧微生物恶臭假单胞菌生产生物可降解高分子材料中长链聚羟基脂肪酸酯mcl-PHA,并将其与模型药结合,测试了PHA单体组成、黏合特性和缓释性能.试验验证了用印染废水中的微生物生产PHA可行,且PHA结构与印染废水种类有关;mcl-PHA可用于后整理的黏合剂、交联剂、混合剂等,对非离子和离子型药剂有良好的混合性.

  17. Is the pie-crusting technique safe for MCL release in varus deformity correction in total knee arthroplasty?

    Science.gov (United States)

    Meneghini, R Michael; Daluga, Andrew T; Sturgis, Lindsey A; Lieberman, Jay R

    2013-09-01

    Established for lateral release in TKA, the pie-crusting technique has not been studied for the medial collateral ligament (MCL). In cadaveric knees the MCL was release with a pie-crusting technique in one and traditional technique in the contralateral knee. Along with a control group, each MCL was subjected to mechanical testing. The stiffness, force and stress required to cause ligament elongation were less in the pie-crusting group (p pie-crusting group demonstrated a characteristic "stair-step" failure mode at the joint line, whereas the traditional group failed elastically at the tibial insertion. MCL pie-crusting is likely technique dependent since failure occurs within the ligament itself and further study is warranted.

  18. Deciphering the Mechanism of Alternative Cleavage and Polyadenylation in Mantle Cell Lymphoma (MCL)

    Science.gov (United States)

    2015-12-01

    Like other hematological malignancies, MCL is characterized by numerous chromosomal translocations. RNA-Sequencing is limited by the relatively...Attend class at CSHL on Deep Sequencing Technology) • Attended CSHL training course in Advanced Sequencing Technology and Applications, November 12-24...226-231. 9. Bertoni F, Zucca E, Cavalli F. Mantle cell lymphoma. Current Opinion in Hematology 2004;11:411-418. 10. Wiestner A, Tehrani M, Chiorazzi

  19. An Examination of Postsecondary Faculty and the Extent of Critical Reading Taught in 100-Level Introductory Biology and American History Courses in Publicly Funded Two-Year and Four-Year Pennsylvania Institutions

    Science.gov (United States)

    Sand, Dianna

    This research examined the responses of postsecondary faculty on a critical reading inventory. The research is quantitative, non-experimental, and incorporates a multiple regression model in the analyses. Three research questions guided this study: (1) By institution type: To what degree does institution type predict the extent to which postsecondary faculty teach critical reading as measured by the Reading Goals Inventory (Jones, 1996)? (2) By faculty status: To what degree does faculty status predict the extent to which postsecondary faculty teach critical reading as measured by the Reading Goals Inventory (Jones, 1996)? (3) By disciplinary area: To what degree does disciplinary area predict the extent to which postsecondary faculty teach critical reading as measured by the Reading Goals Inventory (Jones, 1996)? Faculty from 28 Pennsylvania postsecondary institutions participated in this study. Faculty respondents taught 100-level introductory biology or American history courses either part-time or full-time at Pennsylvania community colleges or Pennsylvania State System of Higher Education (PASSHE) universities. Fifty-four faculty respondents completed the Reading Goals Inventory (Jones, 1996). The researcher conducted multiple regression analyses using a hierarchical method. Predictor variables included Institution Type, Faculty Status, and Disciplinary Area; criterion or outcome variables included seven sub-scales of the critical reading inventory. In this study, Institution Type and Faculty Status were not significant predictors. Disciplinary Area was a consistent significant predictor of the amount of critical reading taught as measured in the Interpretation, Analysis, Evaluation, and Reflection sub-scales of the Reading Goals Inventory (Jones, 1996).

  20. Protein sequences clustering of herpes virus by using Tribe Markov clustering (Tribe-MCL)

    Science.gov (United States)

    Bustamam, A.; Siswantining, T.; Febriyani, N. L.; Novitasari, I. D.; Cahyaningrum, R. D.

    2017-07-01

    The herpes virus can be found anywhere and one of the important characteristics is its ability to cause acute and chronic infection at certain times so as a result of the infection allows severe complications occurred. The herpes virus is composed of DNA containing protein and wrapped by glycoproteins. In this work, the Herpes viruses family is classified and analyzed by clustering their protein-sequence using Tribe Markov Clustering (Tribe-MCL) algorithm. Tribe-MCL is an efficient clustering method based on the theory of Markov chains, to classify protein families from protein sequences using pre-computed sequence similarity information. We implement the Tribe-MCL algorithm using an open source program of R. We select 24 protein sequences of Herpes virus obtained from NCBI database. The dataset consists of three types of glycoprotein B, F, and H. Each type has eight herpes virus that infected humans. Based on our simulation using different inflation factor r=1.5, 2, 3 we find a various number of the clusters results. The greater the inflation factor the greater the number of their clusters. Each protein will grouped together in the same type of protein.

  1. Marinopyrrole derivatives with sulfide spacers as selective disruptors of Mcl-1 binding to pro-apoptotic protein Bim.

    Science.gov (United States)

    Cheng, Chunwei; Liu, Yan; Balasis, Maria E; Garner, Thomas P; Li, Jerry; Simmons, Nicholas L; Berndt, Norbert; Song, Hao; Pan, Lili; Qin, Yong; Nicolaou, K C; Gavathiotis, Evripidis; Sebti, Said M; Li, Rongshi

    2014-07-29

    A series of novel marinopyrroles with sulfide and sulphone spacers were designed and synthesized. Their activity to disrupt the binding of the pro-apoptotic protein, Bim, to the pro-survival proteins, Mcl-1 and Bcl-xL, was evaluated using ELISA assays. Fluorescence-quenching (FQ) assays confirmed the direct binding of marinopyrroles to Mcl-1. Benzyl- and benzyl methoxy-containing sulfide derivatives 4 and 5 were highly potent dual Mcl-1/Bim and Bcl-xL/Bim disruptors (IC50 values of 600 and 700 nM), whereas carboxylate-containing sulfide derivative 9 exhibited 16.4-fold more selectivity for disrupting Mcl-1/Bim over Bcl-xL/Bim binding. In addition, a nonsymmetrical marinopyrrole 12 is as equally potent as the parent marinopyrrole A (1) for disrupting both Mcl-1/Bim and Bcl-xL/Bim binding. Some of the derivatives were also active in intact human breast cancer cells where they reduced the levels of Mcl-1, induced programd cell death (apoptosis) and inhibited cell proliferation.

  2. Marinopyrrole Derivatives with Sulfide Spacers as Selective Disruptors of Mcl-1 Binding to Pro-Apoptotic Protein Bim

    Directory of Open Access Journals (Sweden)

    Chunwei Cheng

    2014-07-01

    Full Text Available A series of novel marinopyrroles with sulfide and sulphone spacers were designed and synthesized. Their activity to disrupt the binding of the pro-apoptotic protein, Bim, to the pro-survival proteins, Mcl-1 and Bcl-xL, was evaluated using ELISA assays. Fluorescence-quenching (FQ assays confirmed the direct binding of marinopyrroles to Mcl-1. Benzyl- and benzyl methoxy-containing sulfide derivatives 4 and 5 were highly potent dual Mcl-1/Bim and Bcl-xL/Bim disruptors (IC50 values of 600 and 700 nM, whereas carboxylate-containing sulfide derivative 9 exhibited 16.4-fold more selectivity for disrupting Mcl-1/Bim over Bcl-xL/Bim binding. In addition, a nonsymmetrical marinopyrrole 12 is as equally potent as the parent marinopyrrole A (1 for disrupting both Mcl-1/Bim and Bcl-xL/Bim binding. Some of the derivatives were also active in intact human breast cancer cells where they reduced the levels of Mcl-1, induced programd cell death (apoptosis and inhibited cell proliferation.

  3. HTLV-1 tax stabilizes MCL-1 via TRAF6-dependent K63-linked polyubiquitination to promote cell survival and transformation.

    Science.gov (United States)

    Choi, Young Bong; Harhaj, Edward William

    2014-10-01

    The human T-cell leukemia virus type 1 (HTLV-1) Tax protein hijacks the host ubiquitin machinery to activate IκB kinases (IKKs) and NF-κB and promote cell survival; however, the key ubiquitinated factors downstream of Tax involved in cell transformation are unknown. Using mass spectrometry, we undertook an unbiased proteome-wide quantitative survey of cellular proteins modified by ubiquitin in the presence of Tax or a Tax mutant impaired in IKK activation. Tax induced the ubiquitination of 22 cellular proteins, including the anti-apoptotic BCL-2 family member MCL-1, in an IKK-dependent manner. Tax was found to promote the nondegradative lysine 63 (K63)-linked polyubiquitination of MCL-1 that was dependent on the E3 ubiquitin ligase TRAF6 and the IKK complex. Tax interacted with and activated TRAF6, and triggered its mitochondrial localization, where it conjugated four carboxyl-terminal lysine residues of MCL-1 with K63-linked polyubiquitin chains, which stabilized and protected MCL-1 from genotoxic stress-induced degradation. TRAF6 and MCL-1 played essential roles in the survival of HTLV-1 transformed cells and the immortalization of primary T cells by HTLV-1. Therefore, K63-linked polyubiquitination represents a novel regulatory mechanism controlling MCL-1 stability that has been usurped by a viral oncogene to precipitate cell survival and transformation.

  4. The C-Type Lectin Receptor MCL Mediates Vaccine-Induced Immunity against Infection with Blastomyces dermatitidis.

    Science.gov (United States)

    Wang, Huafeng; Li, Mengyi; Lerksuthirat, Tassanee; Klein, Bruce; Wüthrich, Marcel

    2015-12-14

    C-type lectin receptors (CLRs) are essential in shaping the immune response to fungal pathogens. Vaccine-induced resistance requires Dectin-2 to promote differentiation of antifungal Th1 and Th17 cells. Since Dectin-2 and MCL heterodimerize and both CLRs use FcRγ as the signaling adaptor, we investigated the role of MCL in vaccine immunity to the fungal pathogen Blastomyces dermatitidis. MCL(-/-) mice showed impaired vaccine resistance against B. dermatitidis infection compared to that of wild-type animals. The lack of resistance correlated with the reduced recruitment of Th17 cells to the lung upon recall following experimental challenge and impaired interleukin-17 (IL-17) production by vaccine antigen-stimulated splenocytes in vitro. Soluble MCL fusion protein recognized and bound a water-soluble ligand from the cell wall of vaccine yeast, but the addition of soluble Dectin-2 fusion protein did not augment ligand recognition by MCL. Taken together, our data indicate that MCL regulates the development of vaccine-induced Th17 cells and protective immunity against lethal experimental infection with B. dermatitidis.

  5. Accounting Faculty Internships

    Directory of Open Access Journals (Sweden)

    Jill Christopher

    2013-06-01

    Full Text Available Accounting professionals, business college accrediting bodies, and even accounting academics themselves acknowledge that there is a disconnect between academe and the rigors and requirements of the accounting profession. Among the suggestions proposed in the literature to reduce this gap is the faculty internship, where accounting faculty members work within the field as accountants. Heretofore, individual case studies report benefits of such internships that accrue to a variety of stakeholder groups beyond just the faculty intern and include the academic institution, students, and accounting profession through faculty internships. This research seeks wider support for these benefits. This descriptive study involved surveying a sample of accounting faculty members to get their opinions about the benefits and drawbacks of faculty internships, and to determine the level of use of faculty internships in accounting. In all, 128 usable responses were obtained, representing a 14.6% response rate. The results of this study reveal that although most faculty members acknowledge the benefits cited in the literature, too few take advantage of faculty internships.

  6. Regulation of Mcl-1 on the Sensitivity to EGFR-TKIs of Non-small Cell Lung Cancer%Mcl-1调控NSCLC对EGFR-TKIs敏感性的实验研究

    Institute of Scientific and Technical Information of China (English)

    李楠; 叶明翔; 张信信; 常宁; 张艰

    2016-01-01

    目的:探讨人髓细胞白血病基因-1(myeloid cell leukemia-1,Mcl-1)是否参与调控非小细胞肺癌(non-small cell lung cancer,NSCLC)对EGFR-TKIs的敏感性,为非小细胞肺癌的治疗提供新的思路.方法:通过Western blot方法检测EGFR-TKIs敏感细胞H3255和耐药细胞H1975中Mcl-1蛋白的表达水平.分别给予敏感细胞H3255和耐药细胞H1975 EGFR-TKIs处理后检测细胞凋亡情况和Mcl-1蛋白表达水平.设计并合成特异性siRNA下调耐药细胞H1975中Mcl-1的表达,采用脂质体转染后通过流式细胞技术检测细胞的凋亡情况.结果:H3255细胞Mcl-1表达水平明显低于H1975细胞.一代EGFR-TKIs Gefitinib显著降低H3255细胞Mcl-1表达而不能减少H1975细胞Mcl-1表达.H1975细胞经二代EGFR-TKIs Afatinib和三代EGFR-TKIs AZD9291处理后Mcl-1表达明显减少.特异性siRNA下调H1975细胞Mcl-1表达可以促进细胞凋亡.结论:Mcl-1参与了调节NSCLC对EGFR-TKIs的敏感性,可能成为防止或逆转NSCLC对EGFR-TKIs耐药的潜在靶点.

  7. A novel BH3 mimetic efficiently induces apoptosis in melanoma cells through direct binding to anti-apoptotic Bcl-2 family proteins, including phosphorylated Mcl-1.

    Science.gov (United States)

    Liu, Yubo; Xie, Mingzhou; Song, Ting; Sheng, Hongkun; Yu, Xiaoyan; Zhang, Zhichao

    2015-03-01

    The Bcl-2 family modulates sensitivity to chemotherapy in many cancers, including melanoma, in which the RAS/BRAF/MEK/ERK pathway is constitutively activated. Mcl-1, a major anti-apoptotic protein in the Bcl-2 family, is extensively expressed in melanoma and contributes to melanoma's well-documented chemoresistance. Here, we provide the first evidence that Mcl-1 phosphorylation at T163 by ERK1/2 and JNK is associated with the resistance of melanoma cell lines to the existing BH3 mimetics gossypol, S1 and ABT-737, and a novel anti-apoptotic mechanism of phosphorylated Mcl-1 (pMcl-1) is revealed. pMcl-1 antagonized the known BH3 mimetics by sequestering pro-apoptotic proteins that were released from Bcl-2/Mcl-1. Furthermore, an anthraquinone BH3 mimetic, compound 6, was identified to be the first small molecule to that induces endogenous apoptosis in melanoma cells by directly binding Bcl-2, Mcl-1, and pMcl-1 and disrupting the heterodimers of these proteins. Although compound 6 induced upregulation of the pro-apoptotic protein Noxa, its apoptotic induction was independent of Noxa. These data reveal the promising therapeutic potential of targeting pMcl-1 to treat melanoma. Compound 6 is therefore a potent drug that targets pMcl-1 in melanoma.

  8. High expression of Mcl-1L via the MEK-ERK-phospho-STAT3 (Ser727) pathway protects melanocytes and melanoma from UVB-induced apoptosis.

    Science.gov (United States)

    Fukumoto, Takeshi; Iwasaki, Tetsushi; Okada, Taro; Hashimoto, Takanori; Moon, Youbin; Sakaguchi, Masanobu; Fukami, Yasuo; Nishigori, Chikako; Oka, Masahiro

    2016-02-01

    Ultraviolet (UV) B is a major factor in melanomagenesis. This fact is linked to the resistance of melanocytes to UVB-induced apoptosis. In this study, we characterized the involvement of Mcl-1L in the regulation of UVB-induced apoptosis in melanocytes and in melanoma cells. In melanocytes, apoptosis was not evident at 24 h after UVB irradiation. The Mcl-1L expression increased after UVB irradiation, and the high Mcl-1L expression continued for at least 24 h. This UVB-dependent increase in Mcl-1L was mediated by the MEK-ERK-pS-STAT3 (STAT3 phosphorylated at Ser727) pathway. The Ser727 phosphorylation facilitated nuclear localization of STAT3. In melanoma cells, the expression levels of Mcl-1L varied depending on the cell line. WM39 melanoma cells expressed high levels of Mcl-1L via the MEK-ERK-pS-STAT3 pathway and were resistant to UVB-induced apoptosis without up-regulation of Mcl-1L. In melanocytes and in WM39 cells, transfection with Mcl-1 siRNA promoted UVB-induced apoptosis. Immunohistochemical studies showed that melanoma cells in in situ lesions expressed high amounts of Mcl-1L. These results indicate that the high expression of Mcl-1L mediated by the MEK-ERK-pS-STAT3 pathway protects melanocytes and melanoma cells from UVB-induced apoptosis.

  9. Expression of MCL-1 and BAK Proteins in Nutritional Anemia and Its Clinical Significance%营养性贫血患者MCL-1和BAK蛋白的表达及其临床意义的研究

    Institute of Scientific and Technical Information of China (English)

    郭玉洁; 王艳; 林凤茹

    2012-01-01

    本研究旨在探讨MCL-1、BAK蛋白与营养性贫血发生发展的关系及其临床意义.采用酶联免疫吸附双抗体夹心法(ELISA)检测66例营养性贫血患者血清中MCL-1、BAK蛋白表达水平.以18例健康体检者为正常对照.结果表明:①营养性贫血组MCL-1蛋白表达水平明显于低正常对照组(P<0.001),BAK蛋白表达水平明显高于正常对照组(P<0.001),不同类型营养性贫血(缺铁性贫血、巨幼细胞性贫血、混合性贫血)各组间MCL-1、BAK蛋白表达水平无明显差异(P>0.05);②营养性贫血组治疗后MCL-1蛋白表达水平明显高于治疗前(P<0.05),BAK蛋白表达水平明显低于治疗前(P<0.05);③营养性贫血患者MCL-1蛋白与BAK蛋白表达水平呈负相关(r=-0.858,P<0.05).结论:MCL-1和BAK蛋白在营养性贫血的发生发展中均有重要作用,并可作为协助诊断营养性贫血及判断预后的参考指标.%This study was aimed to investiage the relation of MCL-1 and BAK proteins with incidence and development of nutritional anemia (NA) and their clinical significance. The MCL-1 and BAK protein levels in serum of 66 patients with NA were determined by using ELISA. Eighteen healthy people were randomly selected as normal controls. The results indicated that; (1)as compared with normal control group, the expression level of MCL-1 protein in 3 NA groups (irorn-deficiency anemia, macrocytic anemia, mixed anemia) significantly decreased (P< 0.001), while the expression level of BAK protein obviously increased (P<0.001), but the expression level of MCL-1 and BAK proteins among 3 NA groups showed no obvious differences; (2) the MCL-1 protein expression level increased and BAK protein expression level decreased in 3 NA groups after treatment (P < 0.05). (3) there was negative correlation of expression levels of MCL-1 protein with BAK protein in NA group (r = - 0. 858 P < 0. 05). It is concluded that the MCL-1 and BAK proteins may play an important

  10. Educational Background and Academic Rank of Faculty Members within US Schools of Pharmacy.

    Science.gov (United States)

    Assemi, Mitra; Hudmon, Karen Suchanek; Sowinski, Kevin M; Corelli, Robin L

    2016-05-25

    Objective. To characterize the educational background and academic rank of faculty members in US schools of pharmacy, estimate the extent to which they are employed by institutions where they received previous training, and determine whether differences in degree origin and rank exist between faculty members in established (≤1995) vs newer programs. Methods. A cross-sectional study was conducted using the American Association of Colleges of Pharmacy (AACP) faculty database and demographic information from the public domain. Results. Among 5516 faculty members, 50.3% held two or more types of degrees. Established schools had a higher median number of faculty members and a higher mean faculty rank than did newer schools. Conclusion. The difference in mean faculty rank highlights the shortage of experienced faculty members in newer schools. Future research efforts should investigate educational attainment in correlation to other faculty and school characteristics and prospectively track and report trends related to pharmacy faculty members composition.

  11. Faculty Compensation Policies.

    Science.gov (United States)

    Silander, Fred

    1983-01-01

    Faculty compensation policy is seen as one means by which an institution influences the faculty to work toward institutional goals. Among the broad criteria for compensation are worth, equity, need, and market measures. Benefits and issues in compensation including differentials in compensation, merit, part-time instruction, etc. are discussed.…

  12. Faculty Retirement Transitions Revitalized

    Science.gov (United States)

    Van Ummersen, Claire; Duranleau, Lauren; McLaughlin, Jean

    2013-01-01

    It has been almost ten years since the American Council on Education (ACE) began to raise awareness of the importance of workplace flexibility in faculty careers and to encourage colleges and universities to support faculty in better integrating their professional and personal lives. With the generous support of the Alfred P. Sloan Foundation, ACE…

  13. Faculty Growth Contracts.

    Science.gov (United States)

    Seldin, Peter

    Growth contracts, described as faculty plans for personal and professional growth proposed by each member of the faculty, are examined. The rationale for growth contracts is explained and a list of some institutions using growth contracts or variations of the concept is provided. Growth contract advantages, the role of the evaluation committee or…

  14. Learner and Faculty Support

    Science.gov (United States)

    Guan, Sharon; Stanford, Daniel

    2016-01-01

    This chapter identifies effective ways to address learner and faculty support. It introduces methods for building a successful learner support system by providing sufficient resources and proactively addressing learner motivation. It also addresses effective faculty support through institutional policies, resources, training, and course…

  15. Adding Breadth and Depth to College and University Residential Communities: A Phenomenological Study of Faculty-in-Residence

    Science.gov (United States)

    Davenport, Amy M.; Pasque, Penny A.

    2014-01-01

    Faculty-in-residence programs in residence halls are unique opportunities for student-faculty involvement, with high levels of commitment from faculty, students, staff, and institutional resources. This hermeneutic phenomenological study explores a faculty-in-residence program at a four-year public university where the FIR program has resulted in…

  16. Vibrationally high-resolved electronic spectra of MCl2 (M = C, Si, Ge, Sn, Pb) and photoelectron spectra of MCl2-

    Science.gov (United States)

    Ran, Yibin; Pang, Min; Shen, Wei; Li, Ming; He, Rongxing

    2016-10-01

    We systematically studied the vibrational-resolved electronic spectra of group IV dichlorides using the Franck-Condon approximation combined with the Duschinsky and Herzberg-Teller effects in harmonic and anharmonic frameworks (only the simulation of absorption spectra includes the anharmonicity). Calculated results showed that the band shapes of simulated spectra are in accordance with those of the corresponding experimental or theoretical ones. We found that the symmetric bend mode in progression of absorption is the most active one, whereas the main contributor in photoelectron spectra is the symmetric stretching mode. Moreover, the Duschinsky and anharmonic effects exert weak influence on the absorption spectra, except for PbCl2 molecule. The theoretical insights presented in this work are significant in understanding the photophysical properties of MCl2 (M = C, Si, Ge, Sn, Pb) and studying the Herzberg-Teller and the anharmonic effects on the absorption spectra of new dichlorides of this main group.

  17. From Theory to Practice: Faculty Training in Business Ethics.

    Science.gov (United States)

    Boatright, John R.

    1991-01-01

    Claims that training business faculty in ethics is a critical component of including ethics in the business curriculum. Includes suggestions concerning what business faculty should know about ethical theory, how to include theory, and curricular and teaching issues. Describes research projects, publications, and workshops. (DK)

  18. Appraisal of Faculty Preparedness by Using Selected Variables.

    Science.gov (United States)

    Rehman, Sajjad ur

    1994-01-01

    Discussion of changes in the criteria by which faculty preparedness is evaluated focuses on a study of six graduate programs of library education in Pakistan that evaluated faculty based on academic qualifications, professional experience, and research and publication credentials. Shortcomings are discussed, and remedial measures are suggested,…

  19. From Theory to Practice: Faculty Training in Business Ethics.

    Science.gov (United States)

    Boatright, John R.

    1991-01-01

    Claims that training business faculty in ethics is a critical component of including ethics in the business curriculum. Includes suggestions concerning what business faculty should know about ethical theory, how to include theory, and curricular and teaching issues. Describes research projects, publications, and workshops. (DK)

  20. Faculty Satisfaction in Higher Education: A TQM Approach

    Science.gov (United States)

    Quraishi, Uzma; Hussain, Ishtiaq; Syed, Makhdoom Ali; Rahman, Farah

    2010-01-01

    This paper was aimed to investigate the levels of satisfaction among faculty members in higher education in Pakistan. Five hundred faculty members were surveyed from leading public and private universities through an instrument developed by the authors and 450 were completed and returned. Percentage method was used to analyze and interpret data.…

  1. Faculty Satisfaction in Higher Education: A TQM Approach

    Science.gov (United States)

    Quraishi, Uzma; Hussain, Ishtiaq; Syed, Makhdoom Ali; Rahman, Farah

    2010-01-01

    This paper was aimed to investigate the levels of satisfaction among faculty members in higher education in Pakistan. Five hundred faculty members were surveyed from leading public and private universities through an instrument developed by the authors and 450 were completed and returned. Percentage method was used to analyze and interpret data.…

  2. Scholarship and the Professional Identity of Community College Faculty Members

    Science.gov (United States)

    Palmer, James C.

    2015-01-01

    The institutional culture of community colleges often fosters a professional identity among faculty members that sees research, publication, and other forms of out-of-class scholarship as detrimental to teaching and student learning. But the professional associations established by and for community college faculty members in specific academic…

  3. A Comprehensive Approach to Orientation and Mentoring for New Faculty.

    Science.gov (United States)

    Keating, Daniel

    1996-01-01

    A Washington University (Missouri) law school program for new faculty has four elements: a publication, "Things I Wish I Had Known as a New Faculty Member"; orientation sessions on teaching, creating and grading exams, pursuing scholarship, and dealing with law reviews; formal mentoring; and a detailed question-and-answer memo about the…

  4. Academic Writing: Supporting Faculty in a Critical Competency for Success

    Science.gov (United States)

    Dankoski, Mary E.; Palmer, Megan M.; Banks, Julianna; Brutkiewicz, Randy R.; Walvoord, Emily; Hoffmann-Longtin, Krista; Bogdewic, Stephen P.; Gopen, George D.

    2012-01-01

    All faculty regardless of discipline or school need to be highly competent at writing for an academic audience. The "publish or perish" pressure is alive and well for academic advancement, publications, and external grant funding. Yet few faculty, particularly in the health professions and sciences, receive formal training on the craft of writing.…

  5. Scientific teaching targeting faculty from diverse institutions.

    Science.gov (United States)

    Gregg, Christopher S; Ales, Jo Dale; Pomarico, Steven M; Wischusen, E William; Siebenaller, Joseph F

    2013-01-01

    We offered four annual professional development workshops called STAR (for Scientific Teaching, Assessment, and Resources) modeled after the National Academies Summer Institute (SI) on Undergraduate Education in Biology. In contrast to the SI focus on training faculty from research universities, STAR's target was faculty from community colleges, 2-yr campuses, and public and private research universities. Because of the importance of community colleges and 2-yr institutions as entries to higher education, we wanted to determine whether the SI model can be successfully extended to this broader range of institutions. We surveyed the four cohorts; 47 STAR alumni responded to the online survey. The responses were separated into two groups based on the Carnegie undergraduate instructional program categories, faculty from seven associate's and associate's-dominant institutions (23) and faculty from nine institutions with primarily 4-yr degree programs (24). Both groups expressed the opinion that STAR had a positive impact on teaching, student learning, and engagement. The two groups reported using techniques of formative assessment and active learning with similar frequency. The mix of faculty from diverse institutions was viewed as enhancing the workshop experience. The present analysis indicates that the SI model for training faculty in scientific teaching can successfully be extended to a broad range of higher education institutions.

  6. Tuning the reactivity of TEMPO by coordination to a Lewis acid: isolation and reactivity of MCl3(η1-TEMPO) (M = Fe, Al).

    Science.gov (United States)

    Scepaniak, Jeremiah J; Wright, Ashley M; Lewis, Richard A; Wu, Guang; Hayton, Trevor W

    2012-11-28

    Addition of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) to MCl(3) (M = Fe, Al) results in the formation of MCl(3)(η(1)-TEMPO) [M = Fe (1), Al (2)]. Both 1 and 2 oxidize alcohols to generate ketones or aldehydes along with the reduced complexes MCl(3)(η(1)-TEMPOH) [M = Fe (3), Al (4)]. Complexes 1-4 were fully characterized, including analysis by X-ray crystallography. Additionally, control experiments indicated that neither MCl(3) (M = Al, Fe) nor TEMPO are capable of effecting the oxidation of alcohols independently.

  7. Lenalidomide-bendamustine-rituximab in untreated mantle cell lymphoma > 65 years, the Nordic Lymphoma Group phase I+II trial NLG-MCL4

    DEFF Research Database (Denmark)

    Albertsson-Lindblad, Alexandra; Kolstad, Arne; Laurell, Anna;

    2016-01-01

    For elderly patients with mantle cell lymphoma (MCL), there is no defined standard therapy. In this multicenter open-label phase I/II trial we evaluated the addition of lenalidomide (LEN) to rituximab-bendamustine (R-B) as first-line treatment to elderly MCL patients. Patients >65 years with untr......For elderly patients with mantle cell lymphoma (MCL), there is no defined standard therapy. In this multicenter open-label phase I/II trial we evaluated the addition of lenalidomide (LEN) to rituximab-bendamustine (R-B) as first-line treatment to elderly MCL patients. Patients >65 years...

  8. Accounting Students' Perceptions of Effective Faculty Attributes

    Science.gov (United States)

    Alfraih, Mishari M.; Alanezi, Faisal S.

    2016-01-01

    Purpose: This study aims to explore the attributes of an effective accounting faculty from the student perspective. It also examines similarities and differences in the perceived importance of these attributes between bachelor's and associate's accounting degree students in two public higher education institutions in Kuwait, namely, Kuwait…

  9. Faculty Workload Issues Connected to Undergraduate Research

    Science.gov (United States)

    Free, Rhona; Griffith, Suzanne; Spellman, Bill

    2015-01-01

    This chapter delineates the consortial activities of the Council of Public Liberal Arts Colleges (COPLAC) to explore models of undergraduate research and to address the impact of undergraduate research on faculty workload. The significant progress made on the member campus of the University of Wisconsin-Superior over the last 10 years is…

  10. Multi-Channel-Learning (MCL - Ein Referenzmodell für Learning-Content-Systeme (LCS

    Directory of Open Access Journals (Sweden)

    Jan vom Brocke

    2005-12-01

    Full Text Available Lerneinheiten müssen stark variierenden Anforderungen gerecht werden. Neben unterschiedlichen Lerntypen spielen vor allem auch die Umfeldbedingungen eine wesentliche Rolle, in denen Lernprozesse stattfinden. Faktoren wie z. B. die Tagesform führen letztlich dazu, dass nicht einmal für eine einzelne Person konstante Lernpräferenzen herrschen.Mit diesem Beitrag wird vorgeschlagen, zur Lösung des Problems einer Mehrkanalstrategie zu folgen. Allerdings sind spezifische Eigenschaften von Learning-Content-Systemen (LCS notwendig, um ein sog. Multi-Channel-Learning (MCL zu ermöglichen. Diese Eigenschaften werden im Beitrag anhand von Informationsmodellen beschrieben werden. Sie sollen als Referenzmodell dienen, das sowohl bei der Entwicklung als auch bei der Auswahl und Anpassung von LCS hilfreich sein kann.Das Referenzmodell wird deduktiv abgeleitet und anhand praktischer Anwendungen geprüft. Vorgestellt werden sowohl Anwendungs- als auch Organisationssysteme, die nach dem Modell realisiert worden sind. Auf dieser Grundlage kann schließlich eine Nutzenabschätzung des Modells für das Multi-Channel-Learnings vorgenommen werden.This paper presents a reference model for the design of learning-content systems (LCS according to a multi-channel strategy.The model comes into play when various requirements for editing learning contents need to be considered. Both the learner's preferences as well as the situative varieties of the learner's context are of utmost importance for the construction of appropriate learning units. One strategy in order to meet these requirements is the editing of content according to a multi-channel strategy. That way, learners can decide via which channel they prefer to deal with the content in a specific situation. The reference model for MCL that is provided with this paper, supports the implementation of such strategies. It gives a description of essential characteristics of LCS in order to facilitate Multi

  11. Liquidus temperatures and phase equilibria in the BaCl2-MCl-BaO systems

    Science.gov (United States)

    Nikolaeva, E. V.; Bovet, A. L.; Korzun, I. V.

    2017-02-01

    The liquidus temperatures of the BaO-BaCl2-MCl systems (with M = alkali metal) are determined by thermal analysis. The caloric effects observed during melting of the barium-containing chloride eutectic with barium oxide additions are studied. A chemical mechanism of barium oxide dissolution in the melts is confirmed. X-ray diffraction patterns taken for the melt solidified after experiment indicate the presence of barium oxychloride Ba4OCl6 in the solid phase. It is shown that the significant increase in the liquidus temperature in adding the barium oxide to barium-containing chloride molten mixtures is related to substantial changes in their composition and structure.

  12. Thermodynamic Assessment of DyCl3-MCl (M=Na, K, Rb, Cs) Systems

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Using the CALPHAD (Calculation of Phase Diagram) technique, the DyCl3-MCl (M=Na, K, Rb, Cs) systems were optimized and calculated. The modified quasi-chemical model in the pair approximation for short-range ordering was used to describe the Gibbs energies of the liquid phase in these systems. From the measured phase diagram data and experimental thermodynamic properties, a series of thermodynamic functions were optimized base on an interactive computer-assisted analysis. The optimized parameters and the experimental data were thermodynamically self-consistent. The optimized results were discussed.

  13. A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.

    Directory of Open Access Journals (Sweden)

    Toyoaki Natsume

    Full Text Available Specialized chromatin exists at centromeres and must be precisely transmitted during DNA replication. The mechanisms involved in the propagation of these structures remain elusive. Fission yeast centromeres are composed of two chromatin domains: the central CENP-A(Cnp1 kinetochore domain and flanking heterochromatin domains. Here we show that fission yeast Mcl1, a DNA polymerase alpha (Pol alpha accessory protein, is critical for maintenance of centromeric chromatin. In a screen for mutants that alleviate both central domain and outer repeat silencing, we isolated several cos mutants, of which cos1 is allelic to mcl1. The mcl1-101 mutation causes reduced CENP-A(Cnp1 in the central domain and an aberrant increase in histone acetylation in both domains. These phenotypes are also observed in a mutant of swi7(+, which encodes a catalytic subunit of Pol alpha. Mcl1 forms S-phase-specific nuclear foci, which colocalize with those of PCNA and Pol alpha. These results suggest that Mcl1 and Pol alpha are required for propagation of centromere chromatin structures during DNA replication.

  14. Apoptosis induced by overall metabolic stress converges on the Bcl-2 family proteins Noxa and Mcl-1.

    Science.gov (United States)

    Wensveen, Felix M; Alves, Nuno L; Derks, Ingrid A M; Reedquist, Kris A; Eldering, Eric

    2011-07-01

    Apoptosis provoked by glucose shortage in dividing T cells is mediated via the BH3-only protein Noxa and inhibition of its binding partner Mcl-1. It is unknown how signals from cellular metabolism can affect the balance between Mcl-1 and Noxa and to what extent other Bcl-2 members are involved in this apoptosis cascade. Here, we defined the mechanism underlying apoptosis in relation to various types of metabolic stress. First, we established that the Noxa/Mcl-1 balance is regulated by glucose deprivation as well as by general metabolic stress, via changes in proteasome-mediated degradation of Mcl-1. Second, in contrast with cytokine-deprivation, no transcriptional modulation of Mcl-1, Puma, Bim or Noxa was observed during glucose deprivation. Third, no changes in PKB or GSK3 activity occurred and no clear role for AMPK was detected. Fourth, apoptosis triggered by nutrient deprivation was executed without signs of overt autophagy and independent of ROS production or p38 MAP kinase activity. Lastly, apoptosis under nutrient limitation could also be delayed by knock-down of Bim or overexpression of Bcl-2. In conclusion, Noxa functions in a specific apoptotic pathway that integrates overall nutrient stress, independent from attenuated PI3K/PKB signaling and without clear involvement of autophagy.

  15. Noxa determines localization and stability of MCL-1 and consequently ABT-737 sensitivity in small cell lung cancer.

    Science.gov (United States)

    Nakajima, W; Hicks, M A; Tanaka, N; Krystal, G W; Harada, H

    2014-02-13

    The sensitivity to ABT-737, a prototype BH3 mimetic drug, varies in a broad range in small cell lung cancer (SCLC) cells. We have previously shown that the expression of Noxa, a BH3-only pro-apoptotic BCL-2 family protein, is the critical determinant of ABT-737 sensitivity. We show here that Noxa regulates the localization and stability of MCL-1, an anti-apoptotic member, which results in modulating ABT-737 sensitivity. Mutations in Noxa within the BH3 domain, the carboxyl terminus mitochondrial targeting domain, or of ubiquitinated lysines not only change the localization and stability of Noxa itself but also affect the mitochondrial localization and phosphorylation/ubiquitination status of MCL-1 and consequently modulate sensitivity to ABT-737. Results of studies utilizing these mutant proteins indicate that Noxa recruits MCL-1 from the cytosol to the mitochondria. Translocation of MCL-1 initiates its phosphorylation and subsequent ubiquitination, which triggers proteasome-mediated degradation. The precise regulatory mechanisms of Noxa/MCL-1 expression and stability could provide alternative targets to modulate apoptosis induced by BH3 mimetic drugs or other chemotherapeutic reagents.

  16. The ratio of Mcl-1 and Noxa determines ABT737 resistance in squamous cell carcinoma of the skin.

    Science.gov (United States)

    Geserick, P; Wang, J; Feoktistova, M; Leverkus, M

    2014-09-11

    Tumour progression and therapy resistance in squamous cell carcinoma of the skin (SCC) is strongly associated with resistance to intrinsic mitochondrial apoptosis. We thus investigated the role of various anti-apoptotic Bcl-2 proteins for apoptosis protection in SCC using the BH3 agonist ABT737 that can overcome multidomain Bcl-2 protein protection. Sensitive SCC cells underwent rapid loss of mitochondrial membrane potential (MMP), subsequent apoptosis concomitant with caspase-3 activation and an early release of mitochondria-derived cytochrome c and smac/DIABLO. In contrast, ABT737 resistance in subsets of SCC cells was not explained by XIAP, important for protection from DR-induced apoptosis in SCC. Of note, ABT737 did not prime SCC cells to DR-induced apoptosis. Interestingly, the ratio of Mcl-1 and Noxa determined sensitivity to ABT737: loss of Mcl-1 rendered resistant cells sensitive to ABT737, whereas loss of Noxa promoted resistance in sensitive cells. In line, suppression of Mcl-1 by the pan-Bcl-2 inhibitor Obatoclax or overexpression of Noxa rendered resistant SCC cells sensitive to BH3 mimetics. Our data indicate that targeting of the Mcl-1/Noxa axis is important to overcome resistance to mitochondrial apoptosis in SCC. Therefore, combination treatment of ABT737 or derivatives with Mcl-1 inhibitors, or inducers of Noxa, may represent a novel option of targeted therapy in metastatic SCC of the skin.

  17. Inhibition of MARCH5 ubiquitin ligase abrogates MCL1-dependent resistance to BH3 mimetics via NOXA.

    Science.gov (United States)

    Subramanian, Aishwarya; Andronache, Adrian; Li, Yao-Cheng; Wade, Mark

    2016-03-29

    BH3 mimetic compounds induce tumor cell death through targeted inhibition of anti-apoptotic BCL2 proteins. Resistance to one such compound, ABT-737, is due to increased levels of anti-apoptotic MCL1. Using chemical and genetic approaches, we show that resistance to ABT-737 is abrogated by inhibition of the mitochondrial RING E3 ligase, MARCH5. Mechanistically, this is due to increased expression of pro-apoptotic BCL2 family member, NOXA, and is associated with MARCH5 regulation of MCL1 ubiquitylation and stability in a NOXA-dependent manner. MARCH5 expression contributed to an 8-gene signature that correlates with sensitivity to the preclinical BH3 mimetic, navitoclax. Furthermore, we observed a synthetic lethal interaction between MCL1 and MARCH5 in MCL1-dependent breast cancer cells. Our data uncover a novel level at which the BCL2 family is regulated; furthermore, they suggest targeting MARCH5-dependent signaling will be an effective strategy for treatment of BH3 mimetic-resistant tumors, even in the presence of high MCL1.

  18. MCL1 and BCL-xL levels in solid tumors are predictive of dinaciclib-induced apoptosis.

    Directory of Open Access Journals (Sweden)

    Robert N Booher

    Full Text Available Dinaciclib is a potent CDK1, 2, 5 and 9 inhibitor being developed for the treatment of cancer. Additional understanding of antitumor mechanisms and identification of predictive biomarkers are important for its clinical development. Here we demonstrate that while dinaciclib can effectively block cell cycle progression, in vitro and in vivo studies, coupled with mouse and human pharmacokinetics, support a model whereby induction of apoptosis is a main mechanism of dinaciclib's antitumor effect and relevant to the clinical duration of exposure. This was further underscored by kinetics of dinaciclib-induced downregulation of the antiapoptotic BCL2 family member MCL1 and correlation of sensitivity with the MCL1-to-BCL-xL mRNA ratio or MCL1 amplification in solid tumor models in vitro and in vivo. This MCL1-dependent apoptotic mechanism was additionally supported by synergy with the BCL2, BCL-xL and BCL-w inhibitor navitoclax (ABT-263. These results provide the rationale for investigating MCL1 and BCL-xL as predictive biomarkers for dinaciclib antitumor response and testing combinations with BCL2 family member inhibitors.

  19. Targeting acute myeloid leukemia by dual inhibition of PI3K signaling and Cdk9-mediated Mcl-1 transcription.

    Science.gov (United States)

    Thomas, Daniel; Powell, Jason A; Vergez, Francois; Segal, David H; Nguyen, Nhu-Y N; Baker, Adele; Teh, Tse-Chieh; Barry, Emma F; Sarry, Jean-Emmanuel; Lee, Erwin M; Nero, Tracy L; Jabbour, Anissa M; Pomilio, Giovanna; Green, Benjamin D; Manenti, Stéphane; Glaser, Stefan P; Parker, Michael W; Lopez, Angel F; Ekert, Paul G; Lock, Richard B; Huang, David C S; Nilsson, Susie K; Récher, Christian; Wei, Andrew H; Guthridge, Mark A

    2013-08-01

    Resistance to cell death is a hallmark of cancer and renders transformed cells resistant to multiple apoptotic triggers. The Bcl-2 family member, Mcl-1, is a key driver of cell survival in diverse cancers, including acute myeloid leukemia (AML). A screen for compounds that downregulate Mcl-1 identified the kinase inhibitor, PIK-75, which demonstrates marked proapoptotic activity against a panel of cytogenetically diverse primary human AML patient samples. We show that PIK-75 transiently blocks Cdk7/9, leading to transcriptional suppression of MCL-1, rapid loss of Mcl-1 protein, and alleviation of its inhibition of proapoptotic Bak. PIK-75 also targets the p110α isoform of PI3K, which leads to a loss of association between Bcl-xL and Bak. The simultaneous loss of Mcl-1 and Bcl-xL association with Bak leads to rapid apoptosis of AML cells. Concordantly, low Bak expression in AML confers resistance to PIK-75-mediated killing. On the other hand, the induction of apoptosis by PIK-75 did not require the expression of the BH3 proteins Bim, Bid, Bad, Noxa, or Puma. PIK-75 significantly reduced leukemia burden and increased the survival of mice engrafted with human AML without inducing overt toxicity. Future efforts to cotarget PI3K and Cdk9 with drugs such as PIK-75 in AML are warranted.

  20. Faculty Mentoring Practices in Academic Emergency Medicine.

    Science.gov (United States)

    Welch, Julie; Sawtelle, Stacy; Cheng, David; Perkins, Tony; Ownbey, Misha; MacNeill, Emily; Hockberger, Robert; Rusyniak, Daniel

    2017-03-01

    Mentoring is considered a fundamental component of career success and satisfaction in academic medicine. However, there is no national standard for faculty mentoring in academic emergency medicine (EM) and a paucity of literature on the subject. The objective was to conduct a descriptive study of faculty mentoring programs and practices in academic departments of EM. An electronic survey instrument was sent to 135 department chairs of EM in the United States. The survey queried faculty demographics, mentoring practices, structure, training, expectations, and outcome measures. Chi-square and Wilcoxon rank-sum tests were used to compare metrics of mentoring effectiveness (i.e., number of publications and National Institutes of Health [NIH] funding) across mentoring variables of interest. Thirty-nine of 135 departments completed the survey, with a heterogeneous mix of faculty classifications. While only 43.6% of departments had formal mentoring programs, many augmented faculty mentoring with project or skills-based mentoring (66.7%), peer mentoring (53.8%), and mentoring committees (18%). Although the majority of departments expected faculty to participate in mentoring relationships, only half offered some form of mentoring training. The mean number of faculty publications per department per year was 52.8, and 11 departments fell within the top 35 NIH-funded EM departments. There was an association between higher levels of perceived mentoring success and both higher NIH funding (p = 0.022) and higher departmental publications rates (p = 0.022). In addition, higher NIH funding was associated with mentoring relationships that were assigned (80%), self-identified (20%), or mixed (22%; p = 0.026). Our findings help to characterize the variability of faculty mentoring in EM, identify opportunities for improvement, and underscore the need to learn from other successful mentoring programs. This study can serve as a basis to share mentoring practices and stimulate

  1. Plagiarism Among Faculty Applicants

    National Research Council Canada - National Science Library

    Beuy Joob; Viroj Wiwanitkit

    2012-01-01

    ....5 Re-education and re-emphasizing the importance of "no plagiarism" in academic work, as well as establishing standards for all present academic faculty members, including senior and administrative...

  2. Faculty Trends and Projected Needs.

    Science.gov (United States)

    Lee, Barbara A.

    1983-01-01

    Matching faculty with projected staffing needs requires creation of a broad-based inventory of faculty talents and interests, but such a database also suggests a number of faculty placement alternatives, including more productive faculty use, increased industry outplacement, and combining academic and nonacademic employment or administrative…

  3. Faculty Trends and Projected Needs.

    Science.gov (United States)

    Lee, Barbara A.

    1983-01-01

    Matching faculty with projected staffing needs requires creation of a broad-based inventory of faculty talents and interests, but such a database also suggests a number of faculty placement alternatives, including more productive faculty use, increased industry outplacement, and combining academic and nonacademic employment or administrative…

  4. Optical properties of 60B2O3–(40–)PbO–MCl2 and 50B2O3–(50–) PbO–MCl2 (M = Pb, Cd) glasses

    Indian Academy of Sciences (India)

    Shajo Sebastian; M Abdul Khadar

    2004-04-01

    Optical absorption and transmittance spectra of 60B2O3–(40–)PbO–MCl2 and 50B2O3–(50–) PbO–MCl2 (M = Pb, Cd) (10 ≤ ≤ 20) glasses of varying composition were recorded in the UV-visible region. Various optical parameters such as optical energy gap (opt), Urbach energy (e), refractive index (0), optical dielectric constant (∝), and ratio of carrier concentration to the effective mass (/*) were determined. The variation of optical energy gap with increase in the concentration of PbCl2 or CdCl2 is discussed.

  5. MCL Plays an Anti-Inflammatory Role in Mycobacterium tuberculosis-Induced Immune Response by Inhibiting NF-κB and NLRP3 Inflammasome Activation

    Directory of Open Access Journals (Sweden)

    Qingwen Zhang

    2017-01-01

    Full Text Available Mycobacterium tuberculosis (Mtb remains a significant menace to global health as it induces granulomatous lung lesions and systemic inflammatory responses during active tuberculosis (TB. Micheliolide (MCL, a sesquiterpene lactone, was recently reported to have a function of relieving LPS-induced inflammatory response, but the regulative role of MCL on the immunopathology of TB still remains unknown. In this experiment, we examined the inhibitory effect of MCL on Mtb-induced inflammatory response in mouse macrophage-like cell line Raw264.7 by downregulating the activation of nuclear factor kappa B (NF-κB and NLRP3 inflammasome. Evidences showed that MCL decreased the secretion of Mtb-induced inflammatory cytokines (IL-1β and TNF-α in a dose-dependent manner. Meanwhile, MCL dramatically suppressed Mtb-induced activation of iNOS and COX2 as well as subsequent production of NO. Furthermore, MCL inhibited Mtb-induced phosphorylation of Akt (Ser 473 in Raw264.7. According to our results, MCL plays an important role in modulating Mtb-induced inflammatory response through PI3K/Akt/NF-κB pathway and subsequently downregulating the activation of NLRP3 inflammasome. Therefore, MCL may represent as a potential drug candidate in the adjuvant treatment of TB by regulating host immune response.

  6. Mcl-1 Is a Novel Target of miR-26b That Is Associated with the Apoptosis Induced by TRAIL in HCC Cells

    Directory of Open Access Journals (Sweden)

    Chunlin Jiang

    2015-01-01

    Full Text Available Aim. To investigate the role of miR-26b and Mcl-1 in TRAIL-inducing cell death in hepatocellular carcinoma. Methods. The expression of miR-26b and Mcl-1 in HCC was detected by RT-qPCR and western blot. The regulation of Mcl-1 by miR-26b was determined by luciferase reporter assay. MTT and flow cytometry were employed to detect the cell viability and apoptosis. Results. miR-26b is commonly downregulated in HCC cell lines compared with the LO2 cell line. In contrast, the Mcl-1 expression is upregulated in HCC cell lines. Bioinformatic analysis identified a putative target site in the Mcl-1 mRNA for miR-26b and luciferase reporter assay showed that miR-26b directly targeted the 3′-UTR (3′-Untranslated Regions of Mcl-1 mRNA. Transfection of miR-26b mimics suppressed Mcl-1 expression in HCC cells and sensitized the cancer cells to TRAIL (tumor necrosis factor-related apoptosis-inducing ligand cytotoxicity. In addition, transfection of HCC cells with Mcl-1 expression plasmid abolished the sensitization effect of miR-26b to TRAIL-inducing apoptosis. Conclusions. Our study showed that miR-26b was a negative regulator of Mcl-1 gene and sensitized TRAIL-inducing apoptosis in HCC cells, suggesting that the miR-26b-Mcl-1 pathway might be a novel target for the treatment of HCC.

  7. Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis.

    Science.gov (United States)

    Singh, Prafull Kumar; Roukounakis, Aristomenis; Frank, Daniel O; Kirschnek, Susanne; Das, Kushal Kumar; Neumann, Simon; Madl, Josef; Römer, Winfried; Zorzin, Carina; Borner, Christoph; Haimovici, Aladin; Garcia-Saez, Ana; Weber, Arnim; Häcker, Georg

    2017-09-01

    The Bcl-2 family protein Bim triggers mitochondrial apoptosis. Bim is expressed in nonapoptotic cells at the mitochondrial outer membrane, where it is activated by largely unknown mechanisms. We found that Bim is regulated by formation of large protein complexes containing dynein light chain 1 (DLC1). Bim rapidly inserted into cardiolipin-containing membranes in vitro and recruited DLC1 to the membrane. Bim binding to DLC1 induced the formation of large Bim complexes on lipid vesicles, on isolated mitochondria, and in intact cells. Native gel electrophoresis and gel filtration showed Bim-containing mitochondrial complexes of several hundred kilodaltons in all cells tested. Bim unable to form complexes was consistently more active than complexed Bim, which correlated with its substantially reduced binding to anti-apoptotic Bcl-2 proteins. At endogenous levels, Bim surprisingly bound only anti-apoptotic Mcl-1 but not Bcl-2 or Bcl-XL, recruiting only Mcl-1 into large complexes. Targeting of DLC1 by RNAi in human cell lines induced disassembly of Bim-Mcl-1 complexes and the proteasomal degradation of Mcl-1 and sensitized the cells to the Bcl-2/Bcl-XL inhibitor ABT-737. Regulation of apoptosis at mitochondria thus extends beyond the interaction of monomers of proapoptotic and anti-apoptotic Bcl-2 family members but involves more complex structures of proteins at the mitochondrial outer membrane, and targeting complexes may be a novel therapeutic strategy. © 2017 Singh et al.; Published by Cold Spring Harbor Laboratory Press.

  8. Preclinical efficacy of sepantronium bromide (YM155) in multiple myeloma is conferred by down regulation of Mcl-1.

    Science.gov (United States)

    Wagner, Verena; Hose, Dirk; Seckinger, Anja; Weiz, Ludmila; Meißner, Tobias; Rème, Thiery; Breitkreutz, Iris; Podar, Klaus; Ho, Anthony D; Goldschmidt, Hartmut; Krämer, Alwin; Klein, Bernard; Raab, Marc S

    2014-11-15

    The inhibitor-of-apoptosis family member survivin has been reported to inhibit apoptosis and regulate mitosis and cytokinesis. In multiple myeloma, survivin has been described to be involved in downstream sequelae of various therapeutic agents. We assessed 1093 samples from previously untreated patients, including two independent cohorts of 392 and 701 patients, respectively. Survivin expression was associated with cell proliferation, adverse prognostic markers, and inferior event-free and overall survival, supporting the evaluation of survivin as a therapeutic target in myeloma. The small molecule suppressant of survivin--YM155--is in clinical development for the treatment of solid tumors. YM155 potently inhibited proliferation and induced apoptosis in primary myeloma cells and cell lines. Gene expression and protein profiling revealed the critical roles of IL6/STAT3-signaling and the unfolded protein response in the efficacy of YM155. Both pathways converged to down regulate anti-apoptotic Mcl-1 in myeloma cells. Conversely, growth inhibition and apoptotic cell death by YM155 was rescued by ectopic expression of Mcl-1 but not survivin, identifying Mcl-1 as the pivotal downstream target of YM155 in multiple myeloma. Mcl-1 expression was likewise associated with adverse prognostic markers, and inferior survival. Our results strongly support the clinical evaluation of YM155 in patients with multiple myeloma.

  9. The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression

    Directory of Open Access Journals (Sweden)

    Demonacos Constantinos

    2010-02-01

    Full Text Available Abstract Background The cyclin-dependent kinase (CDK and mitogen-activated protein kinase (MAPK mediated phosphorylation of glucocorticoid receptor (GR exerts opposite effects on GR transcriptional activity and affects other posttranslational modifications within this protein. The major phosphorylation site of human GR targeted by MAPK family is the serine 226 and multiple kinase complexes phosphorylate receptor at the serine 211 residue. We hypothesize that GR posttranslational modifications are involved in the determination of the cellular fate in human lymphoblastic leukemia cells. We investigated whether UV signalling through alternative GR phosphorylation determined the cell type specificity of glucocorticoids (GCs mediated apoptosis. Results We have identified putative Glucocorticoid Response Elements (GREs within the promoter regulatory regions of the Bcl-2 family members NOXA and Mcl-1 indicating that they are direct GR transcriptional targets. These genes were differentially regulated in CEM-C7-14, CEM-C1-15 and A549 cells by glucocorticoids and JNK pathway. In addition, our results revealed that the S211 phosphorylation was dominant in CEM-C7-14, whereas the opposite was the case in CEM-C1-15 where prevalence of S226 GR phosphorylation was observed. Furthermore, multiple GR isoforms with cell line specific patterns were identified in CEM-C7-14 cells compared to CEM-C1-15 and A549 cell lines with the same antibodies. Conclusions GR phosphorylation status kinetics, and site specificity as well as isoform variability differ in CEM-C7-14, CEM-C1-15, and A549 cells. The positive or negative response to GCs induced apoptosis in these cell lines is a consequence of the variable equilibrium of NOXA and Mcl-1 gene expression potentially mediated by alternatively phosphorylated GR, as well as the balance of MAPK/CDK pathways controlling GR phosphorylation pattern. Our results provide molecular base and valuable knowledge for improving the GC

  10. The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression

    Science.gov (United States)

    2010-01-01

    Background The cyclin-dependent kinase (CDK) and mitogen-activated protein kinase (MAPK) mediated phosphorylation of glucocorticoid receptor (GR) exerts opposite effects on GR transcriptional activity and affects other posttranslational modifications within this protein. The major phosphorylation site of human GR targeted by MAPK family is the serine 226 and multiple kinase complexes phosphorylate receptor at the serine 211 residue. We hypothesize that GR posttranslational modifications are involved in the determination of the cellular fate in human lymphoblastic leukemia cells. We investigated whether UV signalling through alternative GR phosphorylation determined the cell type specificity of glucocorticoids (GCs) mediated apoptosis. Results We have identified putative Glucocorticoid Response Elements (GREs) within the promoter regulatory regions of the Bcl-2 family members NOXA and Mcl-1 indicating that they are direct GR transcriptional targets. These genes were differentially regulated in CEM-C7-14, CEM-C1-15 and A549 cells by glucocorticoids and JNK pathway. In addition, our results revealed that the S211 phosphorylation was dominant in CEM-C7-14, whereas the opposite was the case in CEM-C1-15 where prevalence of S226 GR phosphorylation was observed. Furthermore, multiple GR isoforms with cell line specific patterns were identified in CEM-C7-14 cells compared to CEM-C1-15 and A549 cell lines with the same antibodies. Conclusions GR phosphorylation status kinetics, and site specificity as well as isoform variability differ in CEM-C7-14, CEM-C1-15, and A549 cells. The positive or negative response to GCs induced apoptosis in these cell lines is a consequence of the variable equilibrium of NOXA and Mcl-1 gene expression potentially mediated by alternatively phosphorylated GR, as well as the balance of MAPK/CDK pathways controlling GR phosphorylation pattern. Our results provide molecular base and valuable knowledge for improving the GC based therapies of

  11. Health science center faculty attitudes towards interprofessional education and teamwork.

    Science.gov (United States)

    Gary, Jodie C; Gosselin, Kevin; Bentley, Regina

    2017-10-12

    The attitudes of faculty towards interprofessional education (IPE) and teamwork impact the education of health professions education (HPE) students. This paper reports on a study evaluating attitudes from health professions educators towards IPE and teamwork at one academic health science center (HSC) where modest IPE initiatives have commenced. Drawing from the results of a previous investigation, this study was conducted to examine current attitudes of the faculty responsible for the training of future healthcare professionals. Survey data were collected to evaluate attitudes from HSC faculty, dentistry, nursing, medicine, pharmacy and public health. In general, positive HSC faculty attitudes towards interprofessional learning, education, and teamwork were significantly predicted by those affiliated with the component of nursing. Faculty development aimed at changing attitudes and increasing understanding of IPE and teamwork are critical. Results of this study serve as an underpinning to leverage strengths and evaluate weakness in initiating IPE.

  12. Platinum compounds sensitize ovarian carcinoma cells to ABT-737 by modulation of the Mcl-1/Noxa axis.

    Science.gov (United States)

    Simonin, Karin; N'Diaye, Monique; Lheureux, Stéphanie; Loussouarn, Claire; Dutoit, Soizic; Briand, Mélanie; Giffard, Florence; Brotin, Emilie; Blanc-Fournier, Cécile; Poulain, Laurent

    2013-04-01

    Ovarian cancer is the leading cause of death from gynecological cancer. The anti-apoptotic protein Bcl-x(L) is frequently overexpressed in ovarian carcinoma which correlates with chemotherapy resistance. It has been demonstrated that Bcl-x(L) cooperates with another anti-apoptotic protein, Mcl-1, to protect ovarian cancer cells against apoptosis, and that their concomitant inhibition induces massive cell death. Here, we examined the interest of ABT-737, a potent BH3-mimetic molecule targeting Bcl-x(L), both alone and in combination with Mcl-1 modulators, in ovarian cancer cell lines. As a single agent, ABT-737 was ineffective at promoting cell death in the four cell lines we tested in vitro. However, the specific inhibition of Mcl-1 by siRNA dramatically increased the sensitivity of chemoresistant cells to ABT-737. Platinum compounds also sensitize to ABT-737 by dose-dependently decreasing Mcl-1 expression or by increasing the expression of pro-apoptotic BH3-only proteins Noxa and, to a lower extent, Bim. Furthermore, we demonstrated that Noxa accumulation was involved in apoptosis occurring in response to the combination of ABT-737 and platinum compounds, since cells were protected from apoptosis by its silencing. Moreover, the combination was also highly cytotoxic ex vivo in sliced SKOV3 tumor nodes. However we observed in these slices a strong basal expression of Noxa and apoptotic cell death in response to ABT-737 alone. Therefore, we have revealed that the modulation of the Mcl-1/Noxa axis by platinum compounds results in a strong sensitization of chemoresistant ovarian carcinoma cells to ABT-737, which could constitute a promising therapeutic in these cancers.

  13. Learner-Centered Faculty Development

    Science.gov (United States)

    Yee, Kevin

    2015-01-01

    To maximize their effectiveness, faculty developers should not merely advocate for an active learning approach but also enact it in their own workshops and service-oriented interactions with faculty, even extending to their use of outreach and social media.

  14. Contingent Faculty as Nonideal Workers

    Science.gov (United States)

    Kezar, Adrianna; Bernstein-Sierra, Samantha

    2016-01-01

    This chapter explores how contingent faculty address the issue of work and family and demonstrates the importance of understanding the diversity of contingent faculty experiences and of underemployment rather than notions of the ideal worker to explain their work lives.

  15. Learner-Centered Faculty Development

    Science.gov (United States)

    Yee, Kevin

    2015-01-01

    To maximize their effectiveness, faculty developers should not merely advocate for an active learning approach but also enact it in their own workshops and service-oriented interactions with faculty, even extending to their use of outreach and social media.

  16. The Paradox of Faculty Development

    Science.gov (United States)

    Minter, Robert L.

    2009-01-01

    This paper explores the weaknesses in university faculty development efforts when compared with corporate professional development practices. Suggestions are offered to think of faculty development as a process rather than as isolated development activities.

  17. Faculty-Librarian Partnerships.

    Science.gov (United States)

    Nesbitt, Renee

    1997-01-01

    A survey was designed to foster collaborative development of instructional strategies between education faculty and librarians at a state institution in California. Strategies from the survey results were utilized in developing instructional programs to teach education students information retrieval skills needed to access information from…

  18. Where Are the Faculty?

    Science.gov (United States)

    Hoskins, Barbara J.

    2010-01-01

    This article discusses how faculty members feel about the growth and quality of distance, distributed, and online higher education. The twenty-first century university must innovate to survive as the Internet becomes the dominant source of knowledge and learning. The twenty-first century student connects with information differently than earlier…

  19. Nursing Faculty Members' Perspectives of Faculty-to-Faculty Workplace Incivility among Nursing Faculty Members

    Science.gov (United States)

    Amos, Kimberly S.

    2013-01-01

    In recent years, nursing faculty incivility has been a searing topic of research. Nursing research included studies on incivility among nursing students, incivility between nursing students and nursing faculty, and incivility in the clinical setting. However, literature specifically on nursing faculty incivility was limited. This descriptive,…

  20. Faculty Demand in Higher Education

    Science.gov (United States)

    Rosenthal, Danielle

    2007-01-01

    The objective of this study is to identify the factors that shift the demand curve for faculty at not-for-profit private institutions. It is unique in that to the author's knowledge no other study has directly addressed the question of how the positive correlation between average faculty salaries and faculty-student ratios can be reconciled with…

  1. Faculty Internships for Hospitality Instructors

    Science.gov (United States)

    Lynn, Christine; Hales, Jonathan A; Wiener, Paul

    2007-01-01

    Internships can help hospitality faculty build industry relationships while also ensuring the best and most current training for their students. Many hospitality organizations have structured faculty internships available or are willing to work with faculty to provide individualized internship opportunities. Career and technical educators in…

  2. Colgate University Faculty Handbook, 1970.

    Science.gov (United States)

    Colgate Univ., Hamilton, NY.

    Designed to supplement the university catalog and handbook, this 1970 faculty handbook is primarily for the information and guidance of new members of the faculty. The community is described, and university organization and campus governance outlined. Specific details are reported on various topics, including: faculty use of college facilities,…

  3. Mentoring and Pretenure Faculty Development.

    Science.gov (United States)

    Lowe, Alan A.; And Others

    1991-01-01

    The University of British Columbia (Canada) Dental School uses teaching and research mentors for new faculty, together with a structured semiannual review process, to clearly identify faculty expectations for tenure. Pretenure faculty have appreciated the clear and regular input concerning their progress, and mentors enjoy the interaction with…

  4. Faculty Development: The American Experience.

    Science.gov (United States)

    Seldin, Peter

    A growing movement to improve the quality of teaching has been called "faculty development." It is based on three assumptions: the primary professional activity of most faculty is teaching; instructional behaviors are learned skills, attitudes, and goals; and faculty can be taught how to improve their classroom performance. Interest in faculty…

  5. Faculty Perspectives on Administrator Effectiveness.

    Science.gov (United States)

    Bess, James L.

    The sources of faculty perspectives on the personal effectiveness of administrators are analyzed. It is proposed that faculty will be predisposed to see administrators in different lights, depending on structural elements in decision making and the orientation of the faculty members. Attention is directed to Talcott Parson's theory for classifying…

  6. Legislative Power to Veto Collective Bargaining Agreements by Faculty Unions: An Overlooked Reality?

    Science.gov (United States)

    Henkel, Jan W.; Wood, Norman J.

    1982-01-01

    Discusses Florida and California court decisions on whether collective bargaining agreements negotiated by public university faculty unions are subject to subsequent appropriation of funds by the state legislature. Compares state statutes to ascertain the extent of collective bargaining rights afforded to faculty in public higher education.…

  7. Chronic mast cell leukemia (MCL) with KIT S476I: a rare entity defined by leukemic expansion of mature mast cells and absence of organ damage.

    Science.gov (United States)

    Valent, Peter; Berger, Jörg; Cerny-Reiterer, Sabine; Peter, Barbara; Eisenwort, Gregor; Hoermann, Gregor; Müllauer, Leonhard; Mannhalter, Christine; Steurer, Michael; Bettelheim, Peter; Horny, Hans-Peter; Arock, Michel

    2015-02-01

    Mast cell leukemia (MCL) is a rare, life-threatening malignancy defined by a substantial increase in neoplastic mast cells (MCs) in bone marrow (BM) smears, drug-resistance, and a poor prognosis. In most patients, the survival time is less than 1 year. However, exceptional cases may present with a less malignant course. We report on a 49-year-old female patient with MCL diagnosed in 2013. In February 2013, first symptoms, including flushing, headache, and diarrhea, were recorded. In addition, mild anemia was detected. The disease was characterized by a massive increase in well-granulated, mature, and often spindle-shaped MCs (80 %) in BM smears. The serum tryptase level amounted to 332 ng/mL. Like in most other MCL patients, no skin lesions were detected. However, unlike in other patients, tryptase levels remained stable, and no other signs or symptoms of MCL-induced organ damage were found. Sequencing studies revealed an isolated S476I point mutation in KIT but no mutation in codon 816. The patient received histamine receptor blockers but refused cytoreductive therapy. After 9 months, still no progression or organ damage was detected. However, progression with transformation to acute MCL occurred after 12 months. We propose that the chronic type of MCL with stable conditions, absence of organ damage, and a mature MC morphology is recognized as a distinct entity that should be distinguished from the acute variant of MCL.

  8. Inhibition of Mcl-1 with the pan–Bcl-2 family inhibitor (–)BI97D6 overcomes ABT-737 resistance in acute myeloid leukemia

    Science.gov (United States)

    Pan, Rongqing; Ruvolo, Vivian R.; Wei, Jun; Konopleva, Marina; Reed, John C.; Pellecchia, Maurizio; Ruvolo, Peter P.

    2015-01-01

    Overexpression of antiapoptotic Bcl-2 proteins such as Bcl-2, Bcl-xL, and Mcl-1 is widely associated with tumor initiation, progression, and chemoresistance. Furthermore, it has been demonstrated that Mcl-1 upregulation renders several types of cancers resistant to the Bcl-2/Bcl-xL inhibitors ABT-737 and ABT-263. The emerging importance of Mcl-1 in pathogenesis and drug resistance makes it a high-priority therapeutic target. In this study, we showed that inhibition of Mcl-1 with a novel pan–Bcl-2 inhibitor (–)BI97D6 potently induced apoptosis in acute myeloid leukemia (AML) cells. (–)BI97D6 induced hallmarks of mitochondrial apoptosis, disrupted Mcl-1/Bim and Bcl-2/Bax interactions, and stimulated cell death via the Bak/Bax-dependent mitochondrial apoptosis pathway, suggesting on-target mechanisms. As a single agent, this pan–Bcl-2 inhibitor effectively overcame AML cell apoptosis resistance mediated by Mcl-1 or by interactions with bone marrow mesenchymal stromal cells. (–)BI97D6 was also potent in killing refractory primary AML cells. Importantly, (–)BI97D6 killed AML leukemia stem/progenitor cells while largely sparing normal hematopoietic stem/progenitor cells. These findings demonstrate that pan–Bcl-2 inhibition by an Mcl-1–targeting inhibitor not only overcomes intrinsic drug resistance ensuing from functional redundancy of Bcl-2 proteins, but also abrogates extrinsic resistance caused by the protective tumor microenvironment. PMID:26045609

  9. PROGNOSTICATORS OF JOB SATISFACTION FOR FACULTY IN UNIVERSITIES

    Directory of Open Access Journals (Sweden)

    Jawwad AHMAD

    2012-01-01

    Full Text Available This study examines job satisfaction of teaching faculty working in universities at Pakistan. The study investigates job satisfaction in perspective of gender; organizational commitment; intrinsic and extrinsic rewards; organizational fairness; quality of coworkers’ integration; organizational fairness; and diversity. Data was collected from 203 respondents of 8 public and private sector teaching faculty members. Chi-Square Test, correlation and Ordinary least squares (OLS regression are used to test hypotheses. It is found that there is no significant difference between job satisfaction levels in context of gender; however, extrinsic rewards are primary motivators for job satisfaction of teaching faculty.

  10. Faculty Members in Ad/PR Perceive Discrimination in Academia.

    Science.gov (United States)

    Fedler, Fred; Smith, Ron F.

    This study examined whether faculty in advertising and public relations feel they are being treated fairly in their departments and in the Association for Education in Journalism and Mass Communication (AEJMC). A three-page questionnaire was mailed to 460 people belonging to either the Advertising Division or the Public Relations Division of the…

  11. Booknotes: Chemical Research Faculties: An International Directory

    Science.gov (United States)

    Kauffman, George B.

    1997-08-01

    American Chemical Society: Washington, DC, 1996. xlv + 1248 pp. 22.1x28.2 cm. ISBN 0-8412-3301-2. $199.95 (hb). This comprehensive source of information on research in chemistry and chemistry-related areas conducted by faculty members worldwide in institutions that grant advanced degrees gives the same type of information on an international scale that the ACS Directory of Graduate Research (DGR) (Kauffman, G. B. J. Chem. Educ. 1996, 73, A136) provides for United States and Canadian institutions. Designed to give users sufficient information to locate a colleague, whether known to them or not, by country, academic institution, or name, this new, updated, partially rearranged third edition of Chemical Research Faculties (CRF) contains more than an additional 75 percent of the volume of information in the second (1988) edition (Kauffman, G. B. J. Chem. Educ. 1989, 66, A48). It contains data on 17,370 faculty members (compared to 11,500 in the second edition), with one or two recent representative publications, from 2,182 institutions (compared to 1,922 in the second edition) in 113 countries arranged alphabetically from Afghanistan to Zimbabwe. A minor shortcoming compared to the last edition is the deletion of the index of faculty by research subjects, which, as a contributing editor to several journals, I found useful in locating possible referees with specific areas of expertise.

  12. Faculty development needs.

    Science.gov (United States)

    Houston, Thomas K; Ferenchick, Gary S; Clark, Jeanne M; Bowen, Judith L; Branch, William T; Alguire, Patrick; Esham, Richard H; Clayton, Charles P; Kern, David E

    2004-04-01

    We compared prior training in 4 areas (general teaching skills, teaching specific content areas, teaching by specific methods and in specific settings, and general professional skills) among community-based teachers based in private practices (N = 61) compared with those in community sites operated by teaching institutions (N = 64) and hospital-based faculty (N = 291), all of whom attended one of three national faculty development conferences. The prevalence of prior training was low. Hospital-based faculty reported the most prior training in all 4 categories, teaching hospital affiliated community-based teachers an intermediate amount, and private practice community-based teachers the least (all P <.05). This association remained after multivariable adjustment for age, gender, and amount of time spent in teaching and clinical activities. Preferences for future training reported frequently by the private practice community-based teachers included: time management (48%); teaching evidence-based medicine (46%); evaluation of learners (38%); giving feedback (39%); outpatient precepting (38%); and "teaching in the presence of the patient" (39%).

  13. [Establishment of the first medical faculty in Bosnia and Herzegovina].

    Science.gov (United States)

    Masić, Izet

    2004-01-01

    After establishment of the medical faculties in Zagreb (1917 year), Ljubljana (1919 year) and Belgrade (1921 gear), by the decision of the minister of the National education of the Independent State Croatia from 31.3.1944. year, there were establishment the medical faculties in Sarajevo and Split the same year. The Medical faculty in Sarajevo, as the faculty of the University in Zagreb, officially began with the work 22.11.1944. year by the opening which made the then dean of the Medical faculty in Zagreb professor (doctor) Ante Sercer. For the dean of the Medical faculty in Sarajevo was nominated professor Stanko Sielski, the renown bosnian-herzegovinian physician, the former head of the district bacteriological station in Tuzla and Banja Luka. The access lectures held professor (doctor) Mile Budak, professor (doctor) Ljudevit Thaller, professor (doctor) Ibrahim Ruzdic. The first generation of the students of the school 1944/1945 year numbered about 165 students. The teaching was organized in the complex of the building of the present residance Conac in Sarajevo, and the practical instruction was performed on the basis of the contract with the then General hospital in Sarajevo. The teachers of the faculty were the eminent professors of the Medical faculty at the University in Zagreb. From all the known person, the fact about the so called NDH (IS Croatia) to the medical faculty, for the long time were hidden from the publicity, so that about them was not spoken, till the back several years, either the momentary alive students of the generation. In this article is stated the factographia connected for the establishment and functionioning of the mentioned first medical faculty in Bosnia and Herzegovina.

  14. Rules on intrapair and interpair correlation energy for Cl, Cl- and MCl (M=H, Li, Na, K)

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    According to the calculation results of the intrapair and interpair correlation energy for the title systems, it has been found that the intrapair correlation energy of K shell of Cl is almost a constant and both the intrashell and intershell correlation energy of K and L shell changes little. It has also been found that in MCl series compounds the value of Cl correlation energy contribution depends on the ionicity of MCl compounds, i.e., the Cl correlation energy contribution increases with the increase of the ionic bond strength of the compound and this value is always less than the correlation energy of Cl- anion but always larger than that of Cl atom. These rules are helpful for the estimation of the correlation energy of ionic compounds and the energy changes of chemical reactions.

  15. Prolyl Hydroxylase 3 Attenuates MCL-1-Mediated ATP Production to Suppress the Metastatic Potential of Colorectal Cancer Cells.

    Science.gov (United States)

    Radhakrishnan, Praveenkumar; Ruh, Nadine; Harnoss, Jonathan M; Kiss, Judit; Mollenhauer, Martin; Scherr, Anna-Lena; Platzer, Lisa K; Schmidt, Thomas; Podar, Klaus; Opferman, Joseph T; Weitz, Juergen; Schulze-Bergkamen, Henning; Koehler, Bruno C; Ulrich, Alexis; Schneider, Martin

    2016-04-15

    Hypoxia is a common feature of solid tumors. Prolyl hydroxylase enzymes (PHD1-3) are molecular oxygen sensors that regulate hypoxia-inducible factor activity, but their functions in metastatic disease remain unclear. Here, we assessed the significance of PHD enzymes during the metastatic spread of colorectal cancer. PHD expression analysis in 124 colorectal cancer patients revealed that reduced tumoral expression of PHD3 correlated with increased frequency of distant metastases and poor outcome. Tumorigenicity and metastatic potential of colorectal tumor cells over and underexpressing PHD3 were investigated in orthotopic and heterotopic tumor models. PHD3 overexpression in a syngeneic tumor model resulted in fewer liver metastases, whereas PHD3 knockdown induced tumor spread. The migration of PHD3-overexpressing tumor cells was also attenuated in vitro Conversely, migratory potential and colony formation were enhanced in PHD3-deficient cells, and this phenotype was associated with enhanced mitochondrial ATP production. Furthermore, the effects of PHD3 deficiency were accompanied by increased mitochondrial expression of the BCL-2 family member, member myeloid cell leukemia sequence 1 (MCL-1), and could be reversed by simultaneous inhibition of MCL-1. MCL-1 protein expression was likewise enhanced in human colorectal tumors expressing low levels of PHD3. Therefore, we demonstrate that downregulation of PHD3 augments metastatic spread in human colorectal cancer and identify MCL-1 as a novel downstream effector of oxygen sensing. Importantly, these findings offer new insight into the possible, context-specific deleterious effects of pharmacologic PHD inhibition. Cancer Res; 76(8); 2219-30. ©2016 AACR.

  16. Parkin Sensitizes toward Apoptosis Induced by Mitochondrial Depolarization through Promoting Degradation of Mcl-1

    Directory of Open Access Journals (Sweden)

    Richard G. Carroll

    2014-11-01

    Full Text Available Mitochondrial depolarization promotes Parkin- and PTEN-induced kinase 1 (PINK1-dependent polyubiquitination of multiple proteins on mitochondrial outer membranes, resulting in the removal of defective mitochondria via mitophagy. Because Parkin mutations occur in Parkinson’s disease, a condition associated with the death of dopaminergic neurons in the midbrain, wild-type Parkin is thought to promote neuronal survival. However, here we show that wild-type Parkin greatly sensitized toward apoptosis induced by mitochondrial depolarization but not by proapoptotic stimuli that failed to activate Parkin. Parkin-dependent apoptosis required PINK1 and was efficiently blocked by prosurvival members of the Bcl-2 family or knockdown of Bax and Bak. Upon mitochondrial depolarization, the Bcl-2 family member Mcl-1 underwent rapid Parkin- and PINK1-dependent polyubiquitination and degradation, which sensitized toward apoptosis via opening of the Bax/Bak channel. These data suggest that similar to other sensors of cell stress, such as p53, Parkin has cytoprotective (mitophagy or cytotoxic modes (apoptosis, depending on the degree of mitochondrial damage.

  17. TAZ promotes temozolomide resistance by upregulating MCL-1 in human glioma cells.

    Science.gov (United States)

    Tian, Tian; Li, Aimin; Lu, Hong; Luo, Ran; Zhang, Mingzhi; Li, Zhaoming

    2015-08-07

    Temozolomide is a novel cytotoxic agent currently used as first-line chemotherapy for glioblastoma multiforme (GBM). However, intrinsic or acquired chemoresistance to temozolomide remains the greatest obstacle to the successful treatment of human GBM. The principal mechanism responsible for this resistance is largely unknown. In the present study, we showed that expression of transcriptional co-activator with PDZ-binding motif (TAZ) in glioma cells correlated with temozolomide chemoresistance in human glioma cells. Overexpression of TAZ promoted temozolomide resistance in U-87MG cells, whereas knockdown of TAZ expression sensitized temozolomide-resistant U-251MG cells to temozolomide. Further, TAZ inhibits temozolomide induced apoptosis via upregulation of MCL-1 (myeloid cell leukemia 1) and high expression of TAZ predicts a poor prognosis for GBM patients. In conclusion, our results suggest that TAZ had a critical role in the resistance to temozolomide in glioma cells, and it may provide a promising target for improving the therapeutic outcome of temozolomide-resistant gliomas.

  18. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax.

    Science.gov (United States)

    Blatt, Neal B; Boitano, Anthony E; Lyssiotis, Costas A; Opipari, Anthony W; Glick, Gary D

    2009-10-15

    Bz-423 is a pro-apoptotic 1,4-benzodiazepine with therapeutic properties in murine models of lupus demonstrating selectivity for autoreactive lymphocytes. Bz-423 modulates the F(1)F(0)-ATPase, inducing the formation of superoxide within the mitochondrial respiratory chain, which then functions as a second messenger initiating apoptosis. In order to understand some of the features that contribute to the increased sensitivity of lymphocytes, we report the signaling pathway engaged by Bz-423 in a Burkitt lymphoma cell line (Ramos). Following the generation of superoxide, Bz-423-induced apoptosis requires the activation of Bax and Bak to induce mitochondrial outer membrane permeabilization and cytochrome c release. Knockdown of the BH3-only proteins Bad, Bim, Bik, and Puma inhibits Bz-423 apoptosis, suggesting that these proteins serve as upstream sensors of the oxidant stress induced by Bz-423. Treatment with Bz-423 results in superoxide-dependent Mcl-1 degradation, implicating this protein as the link between Bz-423-induced superoxide and Bax and Bak activation. In contrast to fibroblasts, B cell death induced by Bz-423 is independent of c-Jun N-terminal kinase. These results demonstrate that superoxide generated from the mitochondrial respiratory chain as a consequence of a respiratory transition can signal a specific apoptotic response that differs across cell types.

  19. Downregulation of Mcl-1 through inhibition of translation contributes to benzyl isothiocyanate-induced cell cycle arrest and apoptosis in human leukemia cells.

    Science.gov (United States)

    Zhou, T; Li, G; Cao, B; Liu, L; Cheng, Q; Kong, H; Shan, C; Huang, X; Chen, J; Gao, N

    2013-02-28

    Benzyl isothiocyanate (BITC) is one of the compounds of ITCs' family that has attracted a great deal of interest because of its ability to exhibit anticancer activity. In this study, we investigated the effects of BITC on cell cycle arrest and apoptosis in human leukemia cell lines, primary leukemia cells, and nude mice Jurkat xenograft. Exposure of Jurkat cells to BITC resulted in dose- and time-dependent increase in apoptosis, caspase activation, cytochrome c release, nuclear apoptosis-inducing factor (AIF) accumulation, Bcl2-associated X protein (Bax) translocation, and myeloid cell leukemia-1 (Mcl-1) downregulation. Treatment with these cells also resulted in cell cycle arrest at the G2/M phase. The G2/M-arrested cells are more sensitive to undergoing Mcl-1 downregulation and apoptosis mediated by BITC. BITC downregulates Mcl-1 expression through inhibition of translation, rather than through a transcriptional, post-translational, or caspase-dependent mechanism. Dephosphorylation of eukaryotic initiation factor 4G could contribute to the inhibition of Mcl-1 translation mediated by BITC. Furthermore, ectopic expression of Mcl-1 substantially attenuates BITC-mediated lethality in these cells, whereas knockdown of Mcl-1 through small interfering RNA significantly enhances BITC-mediated lethality. Finally, administration of BITC markedly inhibited tumor growth and induced apoptosis in Jurkat xenograft model in association with the downregulation of Mcl-1. Taken together, these findings represent a novel mechanism by which agents targeting Mcl-1 potentiate BITC lethality in transformed and primary human leukemia cells and inhibitory activity of tumor growth of Jurkat xenograft model.

  20. Alterations in the Noxa/Mcl-1 axis determine sensitivity of small cell lung cancer to the BH3 mimetic ABT-737.

    Science.gov (United States)

    Hauck, Paula; Chao, Bo H; Litz, Julie; Krystal, Geoffrey W

    2009-04-01

    To understand the molecular basis for variable sensitivity to the BH3 mimetic drug ABT-737, the abundance of Bcl-2 family members was assayed in a panel of small cell lung cancer cell lines whose sensitivity varied over a 2-log range. Elevated Noxa and Bcl-2 levels directly correlated with sensitivity to ABT-737, whereas Mcl-1 levels were similar in all cell lines tested regardless of sensitivity. Transgenically enforced expression of Noxa but not Bcl-2 resulted in increased sensitivity to ABT-737 in multiple cell lines. This increase was especially pronounced in the H209 cell line in which expression of Noxa resulted in a proportionate decline in Mcl-1 expression. Although overexpression of Noxa enhanced sensitivity of the H526 and H82 cell lines to ABT-737, it did not result in altered Mcl-1 levels. Similarly, small interfering RNA-mediated knockdown of Noxa expression in the H146 cell line, which increased resistance to ABT-737, did not result in altered Mcl-1 levels. Therefore, three of four cell lines studied failed to show Noxa-mediated regulation of Mcl-1 expression. However, despite failure to regulate Mcl-1 levels, Noxa blocked binding of Bim to Mcl-1 following its release from Bcl-2 by ABT-737. Finally, we observed that a 24-hour incubation of the H526 and WBA cell lines with ABT-737 resulted in increased Noxa expression, suggesting that Noxa may play a direct role in ABT-737-mediated apoptosis. These results indicate that Noxa expression is the critical determinant of ABT-737 sensitivity and loss of Noxa-mediated regulation of Mcl-1 expression may be an important feature of small cell lung cancer biology.

  1. Magnetic and Structural Studies on Two-Dimensional Antiferromagnets (MCl)LaNb2O7 (M = Mn, Co, Cr)

    Science.gov (United States)

    Kitada, Atsushi; Tsujimoto, Yoshihiro; Nishi, Masakazu; Matsuo, Akira; Kindo, Koichi; Ueda, Yutaka; Ajiro, Yoshitami; Kageyama, Hiroshi

    2016-03-01

    We report magnetic and structural studies on the two-dimensional antiferromagnets (MCl)LaNb2O7 (M = Mn, Cr, Co), prepared by topochemical reactions of a layered perovskite RbLaNb2O7. Electron diffraction of these oxyhalides revealed a superstructure with a √{2}a × √{2}a cell for M = Mn and Co, and a 2a × 2a cell for M = Cr, indicating that the MCl networks are distorted from an ideal square lattice. Neutron diffraction experiments showed that M = Mn and Co exhibit a (π 0 π ) antiferromagnetic order as observed for the S = 1/2 counterparts. (CoCl)LaNb2O7 with a strong spin anisotropy shows an antiferro to weak-ferromagnetic transition at low field, followed by novel two-step metamagnetic transitions likely associated with a 1/2 plateau for 27-54 T. Possible spin structures under magnetic field are discussed in terms of an Ising-type model. By contrast, (CrCl)LaNb2O7 exhibits a (π π π ) order, which is the first observation among related oxyhalides, and a spin-flop transition at 12 T due to a weak spin anisotropy. These results suggest that a slight difference in the MCl structure and spin anisotropy provides a crucial influence on the magnetic properties.

  2. miR-193b Modulates Resistance to Doxorubicin in Human Breast Cancer Cells by Downregulating MCL-1

    Directory of Open Access Journals (Sweden)

    Jingpei Long

    2015-01-01

    Full Text Available MicroRNAs (miRNAs family, which is involved in cancer development, proliferation, apoptosis, and drug resistance, is a group of noncoding RNAs that modulate the expression of oncogenes and antioncogenes. Doxorubicin is an active cytotoxic agent for breast cancer treatment, but the acquisition of doxorubicin resistance is a common and critical limitation to cancer therapy. The aim of this study was to investigate whether miR-193b mediated the resistance of breast cancer cells to doxorubicin by targeting myeloid cell leukemia-1 (MCL-1. In this study, we found that miR-193b levels were significantly lower in doxorubicin-resistant MCF-7 (MCF-7/DOXR cells than in the parental MCF-7 cells. We observed that exogenous miR-193b significantly suppressed the ability of MCF-7/DOXR cells to resist doxorubicin. It demonstrated that miR-193b directly targeted MCL-1 3′-UTR (3′-Untranslated Regions. Further studies indicated that miR-193b sensitized MCF-7/DOXR cells to doxorubicin through a mechanism involving the downregulation of MCL-1. Together, our findings provide evidence that the modulation of miR-193b may represent a novel therapeutic target for the treatment of breast cancer.

  3. Antigen-affinity controls pre-germinal centser B cell selection by promoting Mcl-1 induction through BAFF receptor signaling

    Science.gov (United States)

    Wensveen, Felix M.; Slinger, Erik; van Attekum, Martijn HA; Brink, Robert; Eldering, Eric

    2016-01-01

    Upon antigen encounter, the responsive B cell pool undergoes stringent selection which eliminates cells with low B cell receptor (BCR) affinity. Already before formation of the germinal center, activated B cells of low-affinity are negatively selected in a process that is molecularly not well understood. In this study, we investigated the mechanism behind pre-GC affinity-mediated B cell selection. We applied affinity mutants of HEL antigen and found that rapidly after activation B cells become highly dependent on the cytokine BAFF. Moreover, expression of BAFF receptor CD268 is regulated in a BCR-affinity dependent fashion. High affinity responses via BAFF correlated with PI3K activation, which controlled expression of the pro-survival protein Mcl-1, and thereby increased survival. In the presence of excess BAFF, or in absence of the Mcl-1 antagonist Noxa, more low-affinity B cells survived the first two days after antigen encounter. This resulted in increased numbers of antigen-specific B cells of low affinity upon immunization and reduced the overall affinity of cells that contributed to the germinal center reaction. Our findings elucidate a crucial molecular pathway of B cell selection in the earliest phases of activation by identifying a novel link between BCR affinity and BAFF-R signaling towards Mcl-1. PMID:27762293

  4. IL-15 regulates Bcl-2 family members Bim and Mcl-1 through JAK/STAT and PI3K/AKT pathways in T cells.

    Science.gov (United States)

    Shenoy, Aparna R; Kirschnek, Susanne; Häcker, Georg

    2014-08-01

    Maintenance of T cells is determined by their survival capacity, which is regulated by Bcl-2 proteins. Cytokines signalling through the common gamma chains such as IL-2, IL-7 and IL-15 are important for T-cell survival but how these cytokines determine the expression of Bcl-2-family proteins is not clear. We report signalling events of cytokines that regulate expression of two key Bcl-2 proteins, pro-apoptotic Bim and anti-apoptotic Mcl-1, in resting C57BL/6 mouse T cells. IL-2, IL-7 and IL-15 inhibited apoptosis but paradoxically induced the expression of Bim, countered by concomitant induction of Mcl-1. Bim induction by IL-15 was found at the mRNA and protein levels and depended on both JAK/STAT and PI3K signals. A new STAT5-binding site was identified in the Bim promoter, which was occupied by STAT5 upon IL-15 stimulation. Although it also depended on JAK/STAT- and PI3K signalling, Mcl-1 regulation was independent of Mcl-1 mRNA levels and of regulation of protein stability, suggesting translational regulation. Concurrent CD3 signals inhibited some of the IL-7 effect but not the IL-15 effect on Bcl-2 proteins. The data suggest that cytokines induce Bim and prime T cells for apoptosis, but also inhibit apoptosis by stabilising Mcl-1. Later downregulation of short-lived Mcl-1 may induce efficient, Bim-dependent apoptosis.

  5. 3-Bromopyruvate induces apoptosis in breast cancer cells by downregulating Mcl-1 through the PI3K/Akt signaling pathway.

    Science.gov (United States)

    Liu, Zhe; Zhang, Yuan-Yuan; Zhang, Qian-Wen; Zhao, Su-Rong; Wu, Cheng-Zhu; Cheng, Xiu; Jiang, Chen-Chen; Jiang, Zhi-Wen; Liu, Hao

    2014-04-01

    The hexokinase inhibitor 3-bromopyruvate (3-BrPA) can inhibit glycolysis in tumor cells to reduce ATP production, resulting in apoptosis. However, as 3-BrPA is an alkylating agent, its cytotoxic action may be induced by other molecular mechanisms. The results presented here reveal that 3-BrPA-induced apoptosis is caspase independent. Further, 3-BrPA induces the generation of reactive oxygen species in MDA-MB-231 cells, leading to mitochondria-mediated apoptosis. These results suggest that caspase-independent apoptosis may be induced by the generation of reactive oxygen species. In this study, we also demonstrated that 3-BrPA induces apoptosis through the downregulation of myeloid cell leukemia-1 (Mcl-1) in MDA-MB-231 breast cancer cells. The results of Mcl-1 knockdown indicate that Mcl-1 plays an important role in 3-BrPA-induced apoptosis. Further, the upregulation of Mcl-1 expression in 3-BrPA-treated MDA-MB-231 cells significantly increases cell viability. In addition, 3-BrPA treatment resulted in the downregulation of p-Akt, suggesting that 3-BrPA may downregulate Mcl-1 through the phosphoinositide-3-kinase/Akt pathway. These findings indicate that 3-BrPA induces apoptosis in breast cancer cells by downregulating Mcl-1 through the phosphoinositide-3-kinase/Akt signaling pathway.

  6. Junior faculty core curriculum to enhance faculty development.

    Science.gov (United States)

    Guillet, Ronnie; Holloway, Robert G; Gross, Robert A; Libby, Katie; Shapiro, Janine R

    2017-04-01

    Senior Instructors and Assistant Professors in their first academic appointment may not have all the tools for an efficient start to their careers. Although many institutions provide access to mentoring programs and seminars on faculty development, the timing and format of the offerings often conflict with ongoing responsibilities of the faculty, particularly clinical faculty. We established a collaboration between the Clinical and Translational Science Institute (CTSI) and the University of Rochester Medical Center Office for Faculty Development with the goal of developing a week-long Junior Faculty Core Curriculum that would better suit faculty schedules. We convened focus groups and with their help, identified themes for inclusion in the course. Speakers were identified from among local senior faculty. University leadership was enlisted in promoting the course. Individual speakers and course content were evaluated daily, at the end of the week-long course, and 6 months later. Planning for subsequent years incorporated the feedback. Yearly evaluations and subsequent course modification continued. Junior faculty from nearly every department in the Medical Center were represented. There was high learner satisfaction and participation however several limitations were identified and addressed in subsequent years. The focus on principles and available resources, not specific skills or content was appropriate. Daily interactions among participants from a wide variety of departments fostered networking among faculty who may not otherwise have met and discussed common interests. The ultimate value of such an early, intensive faculty development program will depend on whether it equips junior faculty to organize, develop, and achieve their academic goals better than alternative formats. This will require further study.

  7. Career transition and dental school faculty development program.

    Science.gov (United States)

    Hicks, Jeffery L; Hendricson, William D; Partida, Mary N; Rugh, John D; Littlefield, John H; Jacks, Mary E

    2013-11-01

    Academic dentistry, as a career track, is not attracting sufficient numbers of new recruits to maintain a corps of skilled dental educators. The Faculty Development Program (FDP) at the University of Texas Health Science Center at San Antonio Dental School received federal funds to institute a 7-component program to enhance faculty recruitment and retention and provide training in skills associated with success in academics including:(1) a Teaching Excellence and Academic Skills (TExAS)Fellowship, (2) training in research methodology,evidence-based practice research, and information management, (3) an annual dental hygiene faculty development workshop for dental hygiene faculty, (4) a Teaching Honors Program and Academic Dental Careers Fellowship to cultivate students' interest in educational careers, (5) an Interprofessional Primary Care Rotation,(6) advanced education support toward a master's degree in public health, and (7) a key focus of the entire FDP, an annual Career Transition Workshop to facilitate movement from the practice arena to the educational arm of the profession.The Career Transition Workshop is a cap stone for the FDP; its goal is to build a bridge from practice to academic environment. It will provide guidance for private practice, public health, and military dentists and hygienists considering a career transition into academic dentistry. Topics will be addressed including: academic culture, preparation for the academic environment,academic responsibilities, terms of employment,compensation and benefits, career planning, and job search / interviewing. Instructors for the workshop will include dental school faculty who have transitioned from the practice, military, and public health sectors into dental education.Objectives of the Overall Faculty Development Program:• Provide training in teaching and research skills,career planning, and leadership in order to address faculty shortages in dental schools and under representation of minority

  8. Faculty Agency: Departmental Contexts That Matter in Faculty Careers

    Science.gov (United States)

    Campbell, Corbin M.; O'Meara, KerryAnn

    2014-01-01

    In a modern context of constrained resources and high demands, faculty exert agency to strategically navigate their careers (Baez 2000a; Neumann et al. 2006). Guided by the O'Meara et al. (2011) framework on agency in faculty professional lives, this study used Structural Equation Modeling to investigate which departmental factors…

  9. University Faculty Gender Roles Perceptions.

    Science.gov (United States)

    Street, Sue; And Others

    1995-01-01

    Surveyed 400 college faculty men and women to determine gender role preferences and perceptions. Perceptions of the ideal woman, ideal man, most women, most men, and self were measured. Results from the Sex Role Trait Inventory show that both men and women faculty preferences and perceptions were generally very similar. Implications are discussed.…

  10. Faculty Meetings: Hidden Conversational Dynamics

    Science.gov (United States)

    Bowman, Richard F.

    2015-01-01

    In the everydayness of faculty meetings, collegial conversations mirror distinctive dynamics and practices, which either enhance or undercut organizational effectiveness. A cluster of conversational practices affect how colleagues connect, engage, interact, and influence others during faculty meetings in diverse educational settings. The…

  11. Reframing research on faculty development.

    Science.gov (United States)

    O'Sullivan, Patricia S; Irby, David M

    2011-04-01

    Research on faculty development has focused primarily on individual participants and has produced relatively little generalizable knowledge that can guide faculty development programs. In this article, the authors examine how current research on faculty development in medical education can be enriched by research in related fields such as teacher education, quality improvement, continuing medical education, and workplace learning. As a result of this analysis, the authors revise the old model for conceptualizing faculty development (preferably called professional development). This expanded model calls for research on educational process and outcomes focused on two communities of practice: the community created among participants in faculty development programs and the communities of teaching practice in the workplace (classroom or clinic) where teaching actually occurs. For the faculty development community, the key components are the participants, program, content, facilitator, and context in which the program occurs and in which the faculty teach. For the workplace community, associated components include relationships and networks of association in that environment, the organization and culture of the setting, the teaching tasks and activities, and the mentoring available to the members of that academic and/or clinical community of teaching practice. This expanded model of faculty development generates a new set of research questions, which are described along with six recommendations for enhancing research, including establishment of a national center for research in health professions education.

  12. Perspectives on nordic faculty developmet

    DEFF Research Database (Denmark)

    Kolmos, Anette; Vinther, Ole; Andersson, Pernille;

    2004-01-01

    The chapter gives an introduction to the book "Faculty development in nordic engineering" education and describes todays challenges in developing engineering education.......The chapter gives an introduction to the book "Faculty development in nordic engineering" education and describes todays challenges in developing engineering education....

  13. Changing Practices in Faculty Evaluation.

    Science.gov (United States)

    Seldin, Peter

    A guide to understanding and improving faculty evaluation procedures at all types of colleges and universities is presented. The causes of today's crisis in higher education and survival strategies are reviewed, and the search for solvency is related to major changes in assessing faculty performance. The proliferation of court challenges to…

  14. Professorship: A Faculty Development Program.

    Science.gov (United States)

    Davis, Todd M.; Davis, Jane F.

    1987-01-01

    A faculty development program at a traditionally black college was designed to enhance the ability of graduate faculty to supervise research activities of graduate students. Focus was on interpersonal problem solving in advisement and professional issues; classroom techniques of discussion teaching, case methods, and psychodrama encouraged the…

  15. Accreditation: Impact on Faculty Roles.

    Science.gov (United States)

    Costello, Daniel E.

    1994-01-01

    The new mission-linked accreditation standards of the American Assembly of Collegiate Schools of Business represent a fundamental change in how business schools operate. Emphases on strategic planning, stakeholder participation, faculty teams, and continuous improvement will encourage institutional cultural change and help faculty respond with…

  16. Faculty Attitudes about Distance Education

    Science.gov (United States)

    Smidt, Esther; McDyre, Brian; Bunk, Jennifer; Li, Rui; Gatenby, Tanya

    2014-01-01

    In recent years, there has been a dramatic increase in distance learning in higher education. Given this, it is extremely important to understand faculty attitudes about distance education, not only because they can vary widely, but also because it is the faculty, through their design and implementation of online courses, that will shape the…

  17. Faculty Retention in Higher Education

    Science.gov (United States)

    Soomro, Tariq Rahim; Ahmad, Reyaz

    2013-01-01

    Criteria for retaining or firing a highly qualified faculty in higher education in many cases are vague and unclear. This situation is neither a comfortable, nor a healthy, both for the faculty and the administration. Stakeholders have enough reason to blame each other in the absence of transparent mechanism. This paper proposes a transparent…

  18. Arkansas State University Beebe Branch Faculty Handbook.

    Science.gov (United States)

    Arkansas State Univ., Beebe.

    Arkansas State University Beebe Branch provides a liberal arts oriented program for traditional and nontraditional students. Its faculty handbook contains institutional goals, description of responsibilities of administrative officers and faculty committees, faculty employment policies, and administrative and instructional policies. The…

  19. Faculty development for community practitioners.

    Science.gov (United States)

    DeWitt, T G

    1996-12-01

    Developing the academic skills of the individuals who will serve as educators and role models in the community is critical to pediatric resident education in community settings. The main focus of any faculty development program must be on teaching, although for a subset of individuals, the development of research skills should also be a consideration. The three key elements that must be considered for an effective faculty development program include: (1) creating a culture of mutual respect between full-time and community faculty; (2) basing the program on sound principles of education theory, especially adult learning theory, using appropriately trained faculty; and (3) establishing ongoing institutional financial and philosophical support. Effectively addressing these elements should create a faculty development program that will help the community practitioner become an effective role model and practitioner- preceptor-educator.

  20. Finding Win-Win Forms of Economic Development Outreach: Shared Priorities of Business Faculty and Community

    Science.gov (United States)

    Bacdayan, Paul

    2008-01-01

    The mission statements of many public (taxpayer-supported) colleges promise economic development outreach to local business communities. Unfortunately, faculty may be hard-pressed to devote time to outreach. The author looks for specific outreach activities that garner strong support from both faculty and business representatives. The author…

  1. Faculty and Peer Influences on Academic Integrity: College Cheating in Romania

    Science.gov (United States)

    Teodorescu, Daniel; Andrei, Tudorel

    2009-01-01

    The purpose of this research was to examine student perceptions of academic integrity among faculty and peers at a sample of public universities in Romania. The study explores the factors that influence academic dishonesty among college students and compares the relative importance of faculty influences and peer influences on students' intent to…

  2. Female and Male Faculty: Expectations and Interpretations of Presidential Leadership in a Research University.

    Science.gov (United States)

    Johnson, R. Scott

    This study examined what men and women faculty members expect of university presidential leadership, whether men see leadership in terms of authority and hierarchy and whether women look more for community. Using an analysis of discourse, the study interviewed 30 faculty members at a public, research university in the western United States. The…

  3. Faculty and Peer Influences on Academic Integrity: College Cheating in Romania

    Science.gov (United States)

    Teodorescu, Daniel; Andrei, Tudorel

    2009-01-01

    The purpose of this research was to examine student perceptions of academic integrity among faculty and peers at a sample of public universities in Romania. The study explores the factors that influence academic dishonesty among college students and compares the relative importance of faculty influences and peer influences on students' intent to…

  4. Understanding the Full-Time, Non-Tenure-Track Faculty Appointment

    Science.gov (United States)

    Carlucci, Pandora Grewe

    2013-01-01

    This dissertation explores the socialization of full-time, non-tenure-track (FTNTT) faculty members at two U.S. urban, public research universities. The increase in the use of non-tenure-track faculty appointments has been driven by the need to maximize the use of limited resources, while at the same time, address the need for increases in…

  5. Strategic Planning Effectiveness in Jordanian Universities: Faculty Members' and Academic Administrators' Perspectives

    Science.gov (United States)

    Al-Omari, Aieman Ahmad; Salameh, Kayed M.

    2009-01-01

    This study aims to explore the faculty and academic administrators' perception of strategic planning effectiveness (SPE) in a reform environment, measuring the impact of university type, gender, and job role. A total of 338 faculty members and 183 academic administrators who enrolled during the first semester of the 2007-08 term at a public and a…

  6. From the Hospital Room to the Classroom: Recruiting and Supporting New Nursing Faculty

    Science.gov (United States)

    Kersey, Pamela

    2012-01-01

    The health and safety of the public relies heavily on an adequate supply of nurses. The majority of California nurses receive their training in community colleges where the nursing faculty shortage is expected to worsen in the next ten years. The experience of new nursing faculty in community colleges has not been studied or reported in academic…

  7. Finding Win-Win Forms of Economic Development Outreach: Shared Priorities of Business Faculty and Community

    Science.gov (United States)

    Bacdayan, Paul

    2008-01-01

    The mission statements of many public (taxpayer-supported) colleges promise economic development outreach to local business communities. Unfortunately, faculty may be hard-pressed to devote time to outreach. The author looks for specific outreach activities that garner strong support from both faculty and business representatives. The author…

  8. Discovery of 34 g ,mCl (p,γ ) 35Ar resonances activated at classical nova temperatures

    Science.gov (United States)

    Fry, C.; Wrede, C.; Bishop, S.; Brown, B. A.; Chen, A. A.; Faestermann, T.; Hertenberger, R.; Parikh, A.; Pérez-Loureiro, D.; Wirth, H.-F.; García, A.; Ortez, R.

    2015-01-01

    Background: The thermonuclear 34 g ,mCl (p,γ ) 35Ar reaction rates are unknown due to a lack of experimental nuclear physics data. Uncertainties in these rates translate to uncertainties in 34S production in models of classical novae on oxygen-neon white dwarfs. 34S abundances have the potential to aid in the classification of presolar grains. Purpose: Determine resonance energies for the 34 g ,mCl (p,γ ) 35Ar reactions within the region of astrophysical interest for classical novae to a precision of a few keV as an essential first step toward constraining their thermonuclear reaction rates. Method: 35Ar excited states were populated by the 36Ar (d,t ) 35Ar reaction at E (d )=22 MeV and reaction products were momentum analyzed by a high resolution quadrupole-dipole-dipole-dipole (Q3D) magnetic spectrograph. Results: Seventeen new 35Ar levels have been detected at a statistically significant level in the region Ex≈5.9 -6.7 MeV (Er<800 keV ) and their excitation energies have been determined to typical uncertainties of 3 keV. The uncertainties for five previously known levels have also been reduced substantially. The measured level density was compared to those calculated using the WBMB Hamiltonian within the s d -p f model space. Conclusions: Most of the resonances in the region of astrophysical interest have likely been discovered and their energies have been determined, but the resonance strengths are still unknown, and experimentally constraining the 34 g ,mCl (p,γ ) 35Ar reaction rates will require further experiments.

  9. Critical B-lymphoid cell intrinsic role of endogenous MCL-1 in c-MYC-induced lymphomagenesis

    OpenAIRE

    Grabow, S; Kelly, G L; Delbridge, A R D; Kelly, P N; Bouillet, P; Adams, J M; Strasser, A.

    2016-01-01

    Evasion of apoptosis is critical for tumorigenesis, and sustained survival of nascent neoplastic cells may depend upon the endogenous levels of pro-survival BCL-2 family members. Indeed, previous studies using gene-targeted mice revealed that BCL-XL, but surprisingly not BCL-2, is critical for the development of c-MYC-induced pre-B/B lymphomas. However, it remains unclear whether another pro-survival BCL-2 relative contributes to their development. MCL-1 is an intriguing candidate, because it...

  10. Thermodynamic optimization and calculation of phase diagrams of YbCl3-MCl (M=Na, K, Rb, Cs)

    Institute of Scientific and Technical Information of China (English)

    SUN Yimin; YAO Yongxiang; HU Juan; MENG Xiangzhen; GAO Tianyi; QIAO Zhiyu

    2008-01-01

    YbCl3-MCl (M = Na, K, Rb, Cs) systems were optimized and calculated using the CALPHAD (CALculation of PHAse Diagram) technique. The modified quasi-chemical model in the pair-approximation for short-range ordering was used to describe the Gibbs energies of liquid phase in the systems. On the basis of the measured phase diagram data and experimental thermodynamic properties, a series of thermodynamic functions were optimized and calculated through an interactive computer-assisted analysis. Furthermore, some reasonable discussions on the thermodynamic parameters for these strong interaction binary systems were carried out. The results showed that the optimized parameters and experimental data are thermodynamically self-consistent.

  11. Persistent hepatitis C virus (HCV) infection impairs HCV-specific cytotoxic T cell reactivity through Mcl-1/Bim imbalance due to CD127 down-regulation.

    Science.gov (United States)

    Larrubia, J R; Lokhande, M U; García-Garzón, S; Miquel, J; González-Praetorius, A; Parra-Cid, T; Sanz-de-Villalobos, E

    2013-02-01

    In persistent hepatitis C virus (HCV) infection, HCV-specific cytotoxic T lymphocyte (CTL) reactivity is impaired and this affects HCV control. Interleukin-7 receptor (CD127) expression on these cells could regulate CTL reactivity through Mcl-1/Bim balance modulation. Bim is a pro-apoptotic molecule blocked by the action of Mcl-1. Mcl-1/Bim expression and T cell reactivity on HCV-specific CTLs were compared according to CD127 phenotype. Peripheral blood lymphocytes (PBL) from HLA-A2(+) HCV(+) patients were obtained. HCV-specific CTLs were visualized by staining PBL with anti-CD8 and HLA-A2/peptide pentameric complexes (pentamer). Mcl-1/Bim/CD127 phenotype of HCV-specific CTLs was tested by staining detectable CD8(+)/pentamer(+) cells with anti-Mcl-1/Bim/CD127 antibodies. HCV-specific CTL proliferation ability after specific in vitro challenge was tested in the presence and absence of pancaspase inhibitor z-VAD-fmk. All stained cells were analysed by flow cytometry. CD127(low)-expressing HCV-specific CTLs associated with high HCV viraemia, while CD127(high) correlated with undetectable viral loads (P Bim was up-regulated after antigen encounter (P Bim expression on pentamer(+) cells correlated positively with CD127 expression level (P Bim up-regulation after antigen encounter are involved in CD127(low) HCV-specific CTL hyporeactivity during chronic infection, but it can be overcome by apoptosis blockade.

  12. FLAG-induced remission in a patient with acute mast cell leukemia (MCL exhibiting t(7;10(q22;q26 and KIT D816H

    Directory of Open Access Journals (Sweden)

    Peter Valent

    2014-01-01

    Full Text Available Mast cell leukemia (MCL is a life-threatening disease associated with high mortality and drug-resistance. Only few patients survive more than 12 months. We report on a 55-year-old female patient with acute MCL diagnosed in May 2012. The disease was characterized by a rapid increase in white blood cells and mast cells (MC in the peripheral blood, and a rapid increase of serum tryptase levels. The KIT D816H mutation was detected in the blood and bone marrow (BM. Induction chemotherapy with high-dose ARA-C and fludarabine (FLAG was administered. Unexpectedly, the patient entered a hematologic remission with almost complete disappearance of neoplastic MC and a decrease of serum tryptase levels to normal range after 2 cycles of FLAG. Consecutively, the patient was prepared for allogeneic stem cell transplantation. However, shortly after the third cycle of FLAG, tryptase levels increased again, immature MC appeared in the blood, and the patient died from cerebral bleeding. Together, this case shows that intensive chemotherapy regimens, like FLAG, may induce remission in acute MCL. However, treatment responses are short-lived and the overall outcome remains dismal in these patients. We propose to separate this acute type of MCL from more subacute or chronic variants of MCL.

  13. Genetic variants of NOXA and MCL1 modify the risk of HPV16-associated squamous cell carcinoma of the head and neck

    Directory of Open Access Journals (Sweden)

    Zhou Ziyuan

    2012-05-01

    Full Text Available Abstracts Background The cooperation between phorbol 12-myristate 13-acetate induced protein 1 (NOXA and myeloid cell leukemia 1 (MCL1 is critical in the intrinsic apoptotic pathway. Human papillomavirus 16 (HPV16, by inducing p53 and pRb-E2F degradation, may play an essential role in development of squamous cell carcinoma of the head and neck (SCCHN through NOXA-MCL1 axis-mediated apoptosis. Therefore, genetic variants of NOXA and MCL1 may modify the SCCHN risk associated with HPV16 seropositivity. Methods HPV16 serology was obtained by immunoadsorption assay. Four functional SNPs in the promoter of NOXA (rs9957673, rs4558496 and MCL1 (rs9803935, rs3738485 were genotyped for 380 cases and 335 frequency-matched cancer-free controls of non-Hispanic whites. Results Associations between the four polymorphisms and SCCHN risk were not significant, while we observed a significantly joint effect on SCCHN risk between the polymorphisms and HPV16 seropositivity. Notably, this effect modification was particularly pronounced for oropharyngeal cancer in subgroups including never smokers, never drinkers and younger subjects. Conclusions Our results suggested that polymorphisms of NOXA and MCL1 may modify the risk of HPV16-associated oropharyngeal cancer. The further identification of population subgroups at higher risk provides evidence that HPV-targeting treatment may help benefit SCCHN. However, larger studies are needed to validate our findings.

  14. College & University Budgeting. An Introduction for Faculty and Academic Administrators.

    Science.gov (United States)

    Meisinger, Richard J., Jr.; Dubeck, Leroy W.

    A budgeting handbook for academic administrators and faculty is presented. Economic and political influences on budgeting are considered, along with sources of funds for public and private colleges, and the chronology of the budget process. Multiyear summaries of the budget process in different types of colleges are included. Some major policy…

  15. The Information-Seeking Habits of Engineering Faculty

    Science.gov (United States)

    Engel, Debra; Robbins, Sarah; Kulp, Christina

    2011-01-01

    Many studies of information-seeking habits of engineers focus on understanding the similarities and differences between scientists and engineers. This study explores the information-seeking behavior of academic engineering faculty from twenty public research universities. This investigation includes an examination of how frequently engineer- ing…

  16. Community College Faculty: Attitudes toward Guns on Campus

    Science.gov (United States)

    Dahl, Patricia P.; Bonham, Gene, Jr.; Reddington, Frances P.

    2016-01-01

    This exploratory research surveyed faculty who instruct in community colleges from 18 states about their attitudes toward the concealed carry gun policies that allow appropriately licensed citizens to carry a handgun in public places such as college campuses. Building upon previous research involving 4-year institutions, we surveyed 1,889…

  17. Physical Education Pedagogy Faculty Perceptions of Journal Quality

    Science.gov (United States)

    Silverman, Stephen; Kulinna, Pamela Hodges; Phillips, Sharon R.

    2013-01-01

    This study examined perceived journal quality by physical education pedagogy faculty members. Participants (N = 273) were identified in three ways and recruited through e-mail. Based on research in other fields investigating journal quality and on publication patterns in physical education, a web-based survey was used to examine (a) whether…

  18. Faculty Observables and Self-Reported Responsiveness to Academic Dishonesty

    Science.gov (United States)

    Burrus, Robert T., Jr.; Jones, Adam T.; Sackley, William H.; Walker, Michael

    2015-01-01

    Prior to 2009, a mid-sized public institution in the southeast had a faculty-driven honor policy characterized by little education about the policy and no tracking of repeat offenders. An updated code, implemented in August of 2009, required that students sign an honor pledge, created a formal student honor board, and developed a process to track…

  19. The Faculty Subculture, the Librarian Subculture, and Librarians' Scholarly Productivity

    Science.gov (United States)

    Walters, William H.

    2016-01-01

    This study examines the influence of four predictor variables--university-wide research activity, faculty status (eligibility for sabbaticals), university control (public versus private), and enrollment--on the scholarly productivity of librarians at research universities in the United States. University-wide research activity is directly related…

  20. Academic Productivity of Faculty Associated With Craniofacial Surgery Fellowship Programs.

    Science.gov (United States)

    Ruan, Qing Zhao; Ricci, Joseph A; Silvestre, Jason; Ho, Olivia A; Ganor, Oren; Lee, Bernard T

    2017-03-29

    The H-index is increasingly being used as a measure of academic productivity and has been applied to various surgical disciplines. Here the authors calculate the H-index of craniofacial surgery fellowship faculty in North America in order to determine its utility for academic productivity among craniofacial surgeons. A list of fellowship programs was obtained from the website of the American Society of Craniofacial Surgery. Faculty demographics and institution characteristics were obtained from official program websites and the H-index was calculated using Scopus (Elsevier, USA). Data were assessed using bivariate analysis tools (Kruskal-Wallis and Mann-Whitney tests) to determine the relationship between independent variables and career publications, H-index and 5-year H-index (H5-index) of faculty. Dunn test for multiple comparisons was also calculated. A total of 102 faculty members from 29 craniofacial surgery fellowship programs were identified and included. Faculty demographics reflected a median age of 48 (interquartile range [IQR] 13), a predominantly male sample (88/102, 89.7%), and the rank of assistant professor being the most common among faculty members (41/102, 40.2%). Median of career publications per faculty was 37 (IQR 52.5) and medians of H-index and H5-index were 10.0 (IQR 13.75) and 3.5 (IQR 3.25), respectively. Greater age, male gender, Fellow of the American College of Surgeons membership, higher academic rank, and program affiliation with ranked research medical schools were significantly associated with higher H-indices. Variables associated with seniority were positively associated with the H-index. These results suggest that the H-index may be used as an adjunct in determining academic productivity for promotions among craniofacial surgeons.

  1. Faculty as Border Crossers: A Study of Fulbright Faculty

    Science.gov (United States)

    Eddy, Pamela L.

    2014-01-01

    As adult learners, faculty members approach new experiences based on events of the past, but this underlying framework of understanding is challenged when they work abroad for an extended period of time.

  2. Keeping Faculty [Happy]: The Critical Role of a Faculty Center in Developing and Retaining Quality, Collegial Faculty

    Science.gov (United States)

    Hargis, Jace; Gilbertson, Phil

    2010-01-01

    This paper describes an innovative approach to retaining happy and healthy faculty members in a collegial, productive teaching and learning environment. A major portion of the paper shares how the Faculty Center for Teaching and Learning plays a significant role in the faculty interview process, new faculty orientation, and subsequent mentoring of…

  3. Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL Network

    DEFF Research Database (Denmark)

    Klapper, W.; Hoster, E.; Determann, O.;

    2009-01-01

    powerful prognostic biomarker. The pathology panel of the European MCL Network evaluated methods to assess the Ki-67 index including stringent counting, digital image analysis, and estimation by eyeballing. Counting of 2 x 500 lymphoma cells is the gold standard to assess the Ki-67 index since this value.......37 for two methods of digital image analysis, respectively). Counting a reduced number of lymphoma cells (2 x 100 cells) showed high interobserver agreement (CCC = 0.74). Pitfalls of the Ki-67 index are discussed and guidelines and recommendations for assessing the Ki-67 index in MCL are given Udgivelsesdato......Mantle cell lymphoma (MCL) has a heterogeneous clinical course and is mainly an aggressive B cell non-Hodgkin lymphoma; however, there are some indolent cases The Ki-67 index, defined by the percentage of Ki-67-positive lymphoma cells on histopathological slides, has been shown to be a very...

  4. Gender Differences in Business Faculty's Research Motivation

    Science.gov (United States)

    Chen, Yining; Zhao, Qin

    2013-01-01

    The authors use expectancy theory to evaluate gender differences in key factors that motivate faculty to conduct research. Using faculty survey data collected from 320 faculty members at 10 business schools, they found that faculty members, both men and women, who displayed higher motivation were more productive in research. Among them, pretenured…

  5. Academic Incivility among Health Sciences Faculty

    Science.gov (United States)

    Wright, Melissa; Hill, Lilian H.

    2015-01-01

    Academic health centers are under pressure to graduate more health professionals and, therefore, must retain talented faculty members who can educate students in respective disciplines. Faculty-to-faculty incivility is especially relevant to academic medical centers because faculty in the health professions must not only meet university tenure and…

  6. Resources for Developing Senior Faculty as Teachers.

    Science.gov (United States)

    Seal, Robert K.

    1993-01-01

    An annotated list of 38 resources is provided to help administrators, faculty developers, and faculty in designing effective renewal interventions for senior faculty. Topics include research on senior faculty, personnel policies (tenure, growth contracting), program strategies (mentoring, team teaching, motivation), and assessment of institutional…

  7. Sabutoclax, a Mcl-1 Antagonist, Inhibits Tumorigenesis in Transgenic Mouse and Human Xenograft Models of Prostate Cancer

    Directory of Open Access Journals (Sweden)

    Roger S. Jackson, II

    2012-07-01

    Full Text Available Resistance to available therapeutic agents has been a common problem thwarting progress in treatment of castrate-resistant and metastatic prostate cancer (PCa. Overexpression of the Bcl-2 family members, including Mcl-1, in PCa cells is known to inhibit intracellular mitochondrial-dependent apoptosis. Here we report the development of a novel transgenic mouse model that spontaneously develops prostatic intraepithelial neoplasia and adenocarcinoma by the inducible, conditional knockout of transforming growth factor β receptor type II in stromal fibroblastic cells (Tgfbr2ColTKO. The Tgfbr2ColTKO prostate epithelia demonstrated down-regulation of luminal and basal differentiation markers, as well as Pten expression and up-regulation of Mcl-1. However, unlike in men, Tgfbr2ColTKO prostates exhibited no regression acutely after castration. The administration of Sabutoclax (BI-97C1, a pan-active Bcl-2 protein family antagonist mediated apoptosis in castrate-resistant PCa cells of Tgfbr2ColTKO mice and human subcutaneous, orthotopic, and intratibial xenograft PCa models. Interestingly, Sabutoclax had little apoptotic effect on benign prostate tissue in Tgfbr2ColTKO and wild-type mice. Sabutoclax was able to block c-Met activation, a critical axis in PCa metastatic progression. Further, Sabutoclax synergistically sensitized PC-3 cells to the cytotoxic effects of docetaxel (Taxotere. Together, these data suggest that Sabutoclax inhibits castrate-resistant PCa alone at the primary and bone metastatic site as well as support sensitivity to docetaxel treatment.

  8. Retaining nursing faculty beyond retirement age.

    Science.gov (United States)

    Williamson, Marvel L; Cook, Linda; Salmeron, Lois; Burton, Denise

    2010-01-01

    The number of nursing faculty planning to retire by 2020 is alarming. To develop strategies for retaining faculty, researchers asked: What factors influence the decision by nursing faculty to stay in the workforce past retirement age? What barriers could be removed that would encourage faculty to stay longer? Using Giorgi's analysis method, findings from 6 faculty teaching past retirement age revealed key meaning units and grand themes that match Maslow's Hierarchy of Inborn Needs.

  9. Relaxation of the MCL after an Open-Wedge High Tibial Osteotomy results in decreasing contact pressures of the knee over time.

    Science.gov (United States)

    van Egmond, N; Hannink, G; Janssen, D; Vrancken, A C; Verdonschot, N; van Kampen, A

    2017-03-01

    The objective of this study was to investigate the effect of a medial open-wedge osteotomy (OWO) and the release of the superficial medial collateral ligament (MCL) on the tibiofemoral cartilage pressure, the MCL tension and the valgus laxity of the knee. Seven fresh-frozen, human cadaveric knees were used. Medial and lateral mean contact pressure (CP), peak contact pressure (peakCP), and contact area (CA) were measured using a pressure-sensitive film (I-Scan; Tekscan, Boston, MA). The MCL tension was measured using a custom-made device. These measurements were continuously recorded for 5 min after an OWO of 10°. After the osteotomy, the valgus laxity was measured with a handheld Newtonmeter. For one knee, the measurements were continued for 24 h. At the end, a complete release of the superficial MCL was performed and the measurements were repeated at 10°. There was relaxation of the MCL after the osteotomy; the tension dropped in 5 min with 10.7% (mean difference 20.5 N (95% CI 16.1-24.9)), and in 24 h, the tension decreased by 24.2% (absolute difference 38.8 N) (one knee). After the osteotomy, the mean CP, peakCP and CA increased in the medial compartment (absolute difference 0.17 MPa (95% CI 0.14-0.20), 0.27 MPa (95% CI 0.24-0.30), 132.9mm(2) (95% CI 67.7-198.2), respectively), and decreased in the lateral compartment (absolute difference 0.02 MPa (95% CI 0.03 -0.01), 0.08 MPa (95% CI 0.11 - 0.04), 47.0 mm(2) (95% CI -105.8 to 11.8), respectively). Only after a release of the superficial MCL, the mean CP, peak CP and CA significantly decreased in the medial compartment (absolute difference 0.17, 0.27 MPa, 119.8 mm(2), respectively), and increased in the lateral compartment (absolute difference 0.02, 0.11 MPa, 52.4 mm(2), respectively). After the release of the superficial MCL, a mean increase of 7.9° [mean difference - 0.1° (95% CI -1.9 to 1.6)] of the valgus laxity was found. A release of the superficial MCL helps achieve the goal of

  10. A national study on the attitudes of Irish dental faculty members to faculty development.

    LENUS (Irish Health Repository)

    O'Sullivan, E M

    2010-02-01

    International studies suggest that dental faculty are resistant to the concept and practice of faculty development. This paper analyses the demographic and educational profile of Irish Dental Faculty, exploring their attitudes to educational initiatives.

  11. Molecular Monitoring after Autologous Stem Cell Transplantation and Preemptive Rituximab Treatment of Molecular Relapse; Results from the Nordic Mantle Cell Lymphoma Studies (MCL2 and MCL3) with Median Follow-Up of 8.5 Years

    DEFF Research Database (Denmark)

    Kolstad, Arne; Pedersen, Lone Bredo; Eskelund, Christian W

    2017-01-01

    The main objectives of the present study were to monitor minimal residual disease (MRD) in the bone marrow of patients with mantle cell lymphoma (MCL) to predict clinical relapse and guide preemptive treatment with rituximab. Among the patients enrolled in 2 prospective trials by the Nordic...... Lymphoma Group, 183 who had completed autologous stem cell transplantation (ASCT) and in whom an MRD marker had been obtained were included in the our analysis. Fresh samples of bone marrow were analyzed for MRD by a combined standard nested and quantitative real-time PCR assay for Bcl-1/immunoglobulin...... treatment on 1 or more occasions, and in this group, the median time from first molecular relapse to clinical relapse was 55 months. In most cases, rituximab converted patients to MRD negativity (87%), but many patients became MRD-positive again later during follow-up (69%). By multivariate analysis, high...

  12. BCL-x{sub L}/MCL-1 inhibition and RARγ antagonism work cooperatively in human HL60 leukemia cells

    Energy Technology Data Exchange (ETDEWEB)

    Perri, Mariarita; Yap, Jeremy L.; Yu, Jianshi [Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 20 N Pine Street, Baltimore, MD 21201 (United States); Cione, Erika [Department of Pharmacy, Health and Nutritional Sciences, Ed. Polifunzionale, University of Calabria, 87036 Rende, CS (Italy); Fletcher, Steven [Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 20 N Pine Street, Baltimore, MD 21201 (United States); Kane, Maureen A., E-mail: mkane@rx.umaryland.edu [Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 20 N Pine Street, Baltimore, MD 21201 (United States)

    2014-10-01

    The acute promyelocytic leukemia (APL) subtype of acute myeloid leukemia (AML) is characterized by chromosomal translocations that result in fusion proteins, including the promyelocytic leukemia–retinoic acid receptor, alpha fusion protein (PML–RARα). All-trans retinoic acid (atRA) treatment is the standard drug treatment for APL yielding cure rates >80% by activating transcription and proteasomal degradation of retinoic acid receptor, alpha (RARα). Whereas combination therapy with As{sub 2}O{sub 3} has increased survival further, patients that experience relapse and are refractory to atRA and/or As{sub 2}O{sub 3} is a clinically significant problem. BCL-2 family proteins regulate apoptosis and over-expression of anti-apoptotic B-cell leukemia/lymphoma 2 (BCL-2) family proteins has been associated with chemotherapeutic resistance in APL including impairment of the ability of atRA to induce growth arrest and differentiation. Here we investigated the novel BH3 domain mimetic, JY-1-106, which antagonizes the anti-apoptotic BCL-2 family members B-cell lymphoma-extra large (BCL-x{sub L}) and myeloid cell leukemia-1 (MCL-1) alone and in combination with retinoids including atRA, AM580 (RARα agonist), and SR11253 (RARγ antagonist). JY-1-106 reduced cell viability in HL-60 cells alone and in combination with retinoids. The combination of JY-1-106 and SR11253 had the greatest impact on cell viability by stimulating apoptosis. These studies indicate that dual BCL-x{sub L}/MCL-1 inhibitors and retinoids could work cooperatively in leukemia treatment. - Highlights: • Novel Bcl-x{sub L}/Mcl-1 inhibitor JY-1-106 reduces HL60 cell viability. • JY-1-106 is investigated in combination with retinoic acid, AM580, and SR11253. • AM580 is an RARα agonist; SR11253 is an RARγ antagonist. • Combined use of JY-1-106/SR11253 exhibited the greatest cell viability reduction. • JY-1-106 alone or in combination with retinoids induces apoptosis.

  13. Faculty development: principles and practices.

    Science.gov (United States)

    Steinert, Yvonne; Mann, Karen V

    2006-01-01

    Instructors in the health professions today must acquire knowledge and competencies that go beyond disciplinary expertise. It is now generally accepted that educational training as a teacher is essential to a faculty member's effectiveness as an educator. The educational challenges across the health professions share many similarities. In this article, we draw on the medical education literature and focus on faculty development designed to enhance teaching effectiveness. We first address commonly included faculty development topics, including instructional improvement, organizational development, the development of professional academic skills, and the teaching of specific content areas. We then review a variety of educational approaches and formats that are described in the literature. Included in this discussion are commonly used workshops, seminars, short courses, and fellowships, as well as longitudinal programs, peer coaching, mentorship, self-directed learning, and computer-aided instruction. We also briefly explore learning at work and in communities of practice, and we discuss several frequently encountered challenges in designing and implementing faculty development activities, including motivating colleagues and assessing program effectiveness. We conclude the discussion by presenting a set of guidelines for the design of effective faculty development programs.

  14. Czech medical faculties and smoking.

    Science.gov (United States)

    Králíková, E; Kozák, J; Rames, J; Zámecník, L; Wallenfels, I

    1995-05-01

    At the 1st Medical Faculty of Charles University in Prague the prevalence of smoking was investigated among the faculty, staff, students and among health professionals in the country. We found 38.1% smokers (current and occasional) among malephysicians (N = 625), 25.6% smokers among women physicians (N = 394), 48.7% smoking nurses (N = 729) and 42.3% smokers among paramedical staff (N = 298). We have also followed up smoking habits among our students since 1989 (N = 1235). The number of smokers among them rose from 7% in 1989 to 18% in 1994. Students were also asked about their opinion on smoking as a risk factor for coronary heart disease which has a rising trend. Trying to coordinate the anti-smoking activity at all seven medical faculties in the Czech Republic, in collaboration with the Faculty of Medicine of Masaryk University in Brno, the National Centre for Health Promotion and the Czech Commission of EMASH, present the main points of the anti-smoking strategy at Czech medical faculties.

  15. Student narratives of faculty incivility.

    Science.gov (United States)

    Lasiter, Sue; Marchiondo, Lisa; Marchiondo, Kathleen

    2012-01-01

    Academic incivility remains a problem on college campuses. Nursing research has refocused from student impropriety to aberrant faculty behaviors. Our original study using the Nursing Education Environment Survey showed that 133 of 152 student participants experienced uncivil treatment. Latent, inductive content analysis was undertaken to analyze narratives about their "worst experience" of negative faculty behavior. Four categories were identified: "In front of someone," "Talked to others about me," "Made me feel stupid," and "I felt belittled." Incivility had a profound effect on students and is problematic because it increases already significant academic pressure; it interferes with learning and safe clinical performance; it is contrary to caring, a central nursing concept; and it decreases program satisfaction and retention. Few nursing schools have civility policies for faculty behavior. Formal procedures that promote professional interaction should be crafted and implemented. Equally important is creating ways for nursing students to document incivility without fear of retaliation.

  16. Usp9x- and Noxa-mediated Mcl-1 downregulation contributes to pemetrexed-induced apoptosis in human non-small-cell lung cancer cells.

    Science.gov (United States)

    Yan, J; Zhong, N; Liu, G; Chen, K; Liu, X; Su, L; Singhal, S

    2014-07-03

    Pemetrexed, a folate antimetabolite, combined with cisplatin is used as a first-line therapy for malignant pleural mesothelioma (MPM) and locally advanced or metastatic non-small-cell lung cancer (NSCLC). Pemetrexed arrests cell cycle by inhibiting three enzymes in purine and pyrimidine synthesis that are necessary for DNA synthesis. Pemetrexed also promotes apoptosis in target cells, but little is known about its mechanism in cancer cells. We have previously shown that pemetrexed can result in endoplasmic reticulum (ER) stress, and it can lead to downstream apoptosis. In this study, we further elucidate this mechanism. Our data show that pemetrexed increases Noxa expression through activating transcription factor 4 (ATF4) and activating transcription factor 3 (ATF3) upregulation. Furthermore, pemetrexed induces apoptosis by activating the Noxa-Usp9x-Mcl-1 pathway. Inhibition of Noxa by small interfering RNA (siRNA) promotes Usp9x (ubiquitin-specific peptidase 9, X-linked) expression. Moreover, downregulation of the deubiquitinase Usp9x by pemetrexed results in downstream reduction of myeloid cell leukemia 1 (Mcl-1) expression. Mechanistically, Noxa upregulation likely reduces the availability of Usp9x to Mcl-1, thereby promoting its ubiquitination and degradation, leading to the apoptosis of neoplastic cells. Thus, our findings demonstrate that Noxa-Usp9x-Mcl-1 axis may contribute to pemetrexed-induced apoptosis in human lung cancer cells.

  17. CDK1 Inhibition Targets the p53-NOXA-MCL1 Axis, Selectively Kills Embryonic Stem Cells, and Prevents Teratoma Formation

    Directory of Open Access Journals (Sweden)

    Noelle E. Huskey

    2015-03-01

    Full Text Available Embryonic stem cells (ESCs have adopted an accelerated cell-cycle program with shortened gap phases and precocious expression of cell-cycle regulatory proteins, including cyclins and cyclin-dependent kinases (CDKs. We examined the effect of CDK inhibition on the pathways regulating proliferation and survival of ESCs. We found that inhibiting cyclin-dependent kinase 1 (CDK1 leads to activation of the DNA damage response, nuclear p53 stabilization, activation of a subset of p53 target genes including NOXA, and negative regulation of the anti-apoptotic protein MCL1 in human and mouse ESCs, but not differentiated cells. We demonstrate that MCL1 is highly expressed in ESCs and loss of MCL1 leads to ESC death. Finally, we show that clinically relevant CDK1 inhibitors prevent formation of ESC-derived tumors and induce necrosis in established ESC-derived tumors. Our data demonstrate that ES cells are uniquely sensitive to CDK1 inhibition via a p53/NOXA/MCL1 pathway.

  18. RB but not R-HCVAD is a feasible induction regimen prior to auto-HCT in frontline MCL: results of SWOG Study S1106.

    Science.gov (United States)

    Chen, Robert W; Li, Hongli; Bernstein, Steven H; Kahwash, Samir; Rimsza, Lisa M; Forman, Stephen J; Constine, Louis; Shea, Thomas C; Cashen, Amanda F; Blum, Kristie A; Fenske, Timothy S; Barr, Paul M; Phillips, Tycel; Leblanc, Michael; Fisher, Richard I; Cheson, Bruce D; Smith, Sonali M; Faham, Malek; Wilkins, Jennifer; Leonard, John P; Kahl, Brad S; Friedberg, Jonathan W

    2017-03-01

    Aggressive induction chemotherapy followed by autologous haematopoietic stem cell transplant (auto-HCT) is effective for younger patients with mantle cell lymphoma (MCL). However, the optimal induction regimen is widely debated. The Southwestern Oncology Group S1106 trial was designed to assess rituximab plus hyperCVAD/MTX/ARAC (hyperfractionated cyclophosphamide, vincristine, doxorubicin and dexamethasone, alternating with high dose cytarabine and methotrexate) (RH) versus rituximab plus bendamustine (RB) in a randomized phase II trial to select a pre-transplant induction regimen for future development. Patients had previously untreated stage III, IV, or bulky stage II MCL and received either 4 cycles of RH or 6 cycles of RB, followed by auto-HCT. Fifty-three of a planned 160 patients were accrued; an unacceptably high mobilization failure rate (29%) on the RH arm prompted premature study closure. The estimated 2-year progression-free survival (PFS) was 81% vs. 82% and overall survival (OS) was 87% vs. 88% for RB and RH, respectively. RH is not an ideal platform for future multi-centre transplant trials in MCL. RB achieved a 2-year PFS of 81% and a 78% MRD negative rate. Premature closure of the study limited the sample size and the precision of PFS estimates and MRD rates. However, RB can achieve a deep remission and could be a platform for future trials in MCL. © 2016 John Wiley & Sons Ltd.

  19. Clitocine targets Mcl-1 to induce drug-resistant human cancer cell apoptosis in vitro and tumor growth inhibition in vivo.

    Science.gov (United States)

    Sun, Jian-Guo; Li, Hua; Li, Xia; Zeng, Xueli; Wu, Ping; Fung, Kwok-Pui; Liu, Fei-Yan

    2014-05-01

    Drug resistance is a major reason for therapy failure in cancer. Clitocine is a natural amino nucleoside isolated from mushroom and has been shown to inhibit cancer cell proliferation in vitro. In this study, we observed that clitocine can effectively induce drug-resistant human cancer cell apoptosis in vitro and inhibit tumor xenograft growth in vivo. Clitocine treatment inhibited drug-resistant human cancer cell growth in vitro in a dose- and time-dependent manner. Biochemical analysis revealed that clitocine-induced tumor growth inhibition is associated with activation of caspases 3, 8 and 9, PARP cleavage, cytochrome c release and Bax, Bak activation, suggesting that clitocine inhibits drug-resistant cancer cell growth through induction of apoptosis. Analysis of apoptosis regulatory genes indicated that Mcl-1 level was dramatically decreased after clitocine treatment. Over-expression of Mcl-1 reversed the activation of Bax and attenuated clitocine-induced apoptosis, suggesting that clitocine-induced apoptosis was at least partially by inducing Mcl-1 degradation to release Bax and Bak. Consistent with induction of apoptosis in vitro, clitocine significantly suppressed the drug-resistant hepatocellular carcinoma xenograft growth in vivo by inducing apoptosis as well as inhibiting cell proliferation. Taken together, our data demonstrated that clitocine is a potent Mcl-1 inhibitor that can effectively induce apoptosis to suppress drug-resistant human cancer cell growth both in vitro and in vivo, and thus holds great promise for further development as potentially a novel therapeutic agent to overcome drug resistance in cancer therapy.

  20. JAK2V617F drives Mcl-1 expression and sensitizes hematologic cell lines to dual inhibition of JAK2 and Bcl-xL.

    Directory of Open Access Journals (Sweden)

    Jun Guo

    Full Text Available Constitutive activation of the Janus kinase (JAK/signal transducer and activator of transcription (STAT axis is fundamental to the molecular pathogenesis of a host of hematological disorders, including acute leukemias and myeloproliferative neoplasms (MPN. We demonstrate here that the major JAK2 mutation observed in these diseases (JAK2V617F enforces Mcl-1 transcription via STAT3 signaling. Targeting this lesion with JAK inhibitor I (JAKi-I attenuates STAT3 binding to the Mcl-1 promoter and suppresses Mcl-1 transcript and protein expression. The neutralization of Mcl-1 in JAK2V617F-harboring myelodyssplastic syndrome cell lines sensitizes them to apoptosis induced by the BH3-mimetic and Bcl-xL/Bcl-2 inhibitor, ABT-263. Moreover, simultaneously targeting JAK and Bcl-xL/-2 is synergistic in the presence of the JAK2V617F mutation. These findings suggest that JAK/Bcl-xL/-2 inhibitor combination therapy may have applicability in a range of hematological disorders characterized by activating JAK2 mutations.

  1. Crosstalk among Bcl-2 family members in B-CLL: seliciclib acts via the Mcl-1/Noxa axis and gradual exhaustion of Bcl-2 protection.

    Science.gov (United States)

    Hallaert, D Y H; Spijker, R; Jak, M; Derks, I A M; Alves, N L; Wensveen, F M; de Boer, J P; de Jong, D; Green, S R; van Oers, M H J; Eldering, E

    2007-11-01

    Seliciclib (R-roscovitine) is a cyclin-dependent kinase inhibitor in clinical development. It triggers apoptosis by inhibiting de novo transcription of the short-lived Mcl-1 protein, but it is unknown how this leads to Bax/Bak activation that is required for most forms of cell death. Here, we studied the effects of seliciclib in B-cell chronic lymphocytic leukemia (B-CLL), a malignancy with aberrant expression of apoptosis regulators. Although seliciclib-induced Mcl-1 degradation within 4 h, Bax/Bak activation occurred between 16 and 20 h. During this period, no transcriptional changes in apoptosis-related genes occurred. In untreated cells, prosurvival Mcl-1 was engaged by the proapoptotic proteins Noxa and Bim. Upon drug treatment, Bim was quickly released. The contribution of Noxa and Bim as a specific mediator of seliciclib-induced apoptosis was demonstrated via RNAi. Significantly, 16 h after seliciclib treatment, there was accumulation of Bcl-2, Bim and Bax in the 'mitochondria-rich' insoluble fraction of the cell. This suggests that after Mcl-1 degradation, the remaining apoptosis neutralizing capacity of Bcl-2 is gradually overwhelmed, until Bax forms large multimeric pores in the mitochondria. These data demonstrate in primary leukemic cells hierarchical binding and crosstalk among Bcl-2 members, and suggest that their functional interdependence can be exploited therapeutically.

  2. Exposure to the viral by-product dsRNA or Coxsackievirus B5 triggers pancreatic beta cell apoptosis via a Bim / Mcl-1 imbalance.

    Directory of Open Access Journals (Sweden)

    Maikel L Colli

    2011-09-01

    Full Text Available The rise in type 1 diabetes (T1D incidence in recent decades is probably related to modifications in environmental factors. Viruses are among the putative environmental triggers of T1D. The mechanisms regulating beta cell responses to viruses, however, remain to be defined. We have presently clarified the signaling pathways leading to beta cell apoptosis following exposure to the viral mimetic double-stranded RNA (dsRNA and a diabetogenic enterovirus (Coxsackievirus B5. Internal dsRNA induces cell death via the intrinsic mitochondrial pathway. In this process, activation of the dsRNA-dependent protein kinase (PKR promotes eIF2α phosphorylation and protein synthesis inhibition, leading to downregulation of the antiapoptotic Bcl-2 protein myeloid cell leukemia sequence 1 (Mcl-1. Mcl-1 decrease results in the release of the BH3-only protein Bim, which activates the mitochondrial pathway of apoptosis. Indeed, Bim knockdown prevented both dsRNA- and Coxsackievirus B5-induced beta cell death, and counteracted the proapoptotic effects of Mcl-1 silencing. These observations indicate that the balance between Mcl-1 and Bim is a key factor regulating beta cell survival during diabetogenic viral infections.

  3. Anxiolytic-like effect of a selective and non-peptidergic melanocortin 4 receptor antagonist, MCL0129, in a social interaction test.

    Science.gov (United States)

    Shimazaki, Toshiharu; Chaki, Shigeyuki

    2005-03-01

    The social interaction test is an animal behavioral test of anxiety. Brain melanocortins such as alpha-melanocyte stimulating hormone (alpha-MSH) and adrenocorticotropic hormone (ACTH) have anxiogenic effects in this test. Melanocortins have five receptor subtypes (MC1-MC5). Among them, MC3 and MC4 receptor are mainly expressed in the brain. We investigated the involvement of MC4 receptor in a social interaction test, using Ac-[Nle(4),Asp(5),D-Phe(7),Lys(10)]alpha-MSH-(4-10)-NH2 (MT II), an MC4 receptor agonist, and 1-[(S)-2-(4-fluorophenyl)-2-(4-isopropylpiperadin-1-yl)ethyl]-4-[4-(2-methoxynaphthalen-1-yl)butyl]piperazine (MCL0129), a selective and nonpeptide MC4 receptor antagonist. MT II dose-dependently and significantly reduced the time spent in social interaction. Acute administration of MCL0129 had no effect on the results of this test. In contrast, when given repeatedly for 1 week, MCL0129 significantly and dose-dependently increased the time spent in social interaction without affecting locomotor activity. These results suggest that MC4 receptor is involved in social interaction, and that MCL0129, an MC4 receptor antagonist, has an anxiolytic-like effect in this model.

  4. Exposure to the viral by-product dsRNA or Coxsackievirus B5 triggers pancreatic beta cell apoptosis via a Bim / Mcl-1 imbalance.

    Directory of Open Access Journals (Sweden)

    Maikel L Colli

    2011-09-01

    Full Text Available The rise in type 1 diabetes (T1D incidence in recent decades is probably related to modifications in environmental factors. Viruses are among the putative environmental triggers of T1D. The mechanisms regulating beta cell responses to viruses, however, remain to be defined. We have presently clarified the signaling pathways leading to beta cell apoptosis following exposure to the viral mimetic double-stranded RNA (dsRNA and a diabetogenic enterovirus (Coxsackievirus B5. Internal dsRNA induces cell death via the intrinsic mitochondrial pathway. In this process, activation of the dsRNA-dependent protein kinase (PKR promotes eIF2α phosphorylation and protein synthesis inhibition, leading to downregulation of the antiapoptotic Bcl-2 protein myeloid cell leukemia sequence 1 (Mcl-1. Mcl-1 decrease results in the release of the BH3-only protein Bim, which activates the mitochondrial pathway of apoptosis. Indeed, Bim knockdown prevented both dsRNA- and Coxsackievirus B5-induced beta cell death, and counteracted the proapoptotic effects of Mcl-1 silencing. These observations indicate that the balance between Mcl-1 and Bim is a key factor regulating beta cell survival during diabetogenic viral infections.

  5. MCL-1 is required throughout B-cell development and its loss sensitizes specific B-cell subsets to inhibition of BCL-2 or BCL-XL

    NARCIS (Netherlands)

    Vikström, Ingela B; Slomp, Anne; Carrington, Emma M; Moesbergen, Laura M; Chang, Catherine; Kelly, Gemma L; Glaser, Stefan P; Jansen, J H Marco; Leusen, Jeanette H W; Strasser, Andreas; Huang, David C S; Lew, Andrew M; Peperzak, Victor; Tarlinton, David M

    2016-01-01

    Pro-survival BCL-2 family members protect cells from programmed cell death that can be induced by multiple internal or external cues. Within the haematopoietic lineages, the BCL-2 family members BCL-2, BCL-XL and MCL-1 are known to support cell survival but the individual and overlapping roles of th

  6. MCL and mincle: C-type lectin receptors that sense damaged self and pathogen associated molecular patterns

    Directory of Open Access Journals (Sweden)

    Mark B Richardson

    2014-06-01

    Full Text Available MCL (macrophage C-type lectin and mincle (macrophage inducible C-type lectin comprise part of an extensive repertoire of pattern recognition receptors with the ability to sense damage associated and pathogen associated molecular patterns. In this review we cover the discovery and molecular characterization of these C-type lectin receptors, and highlight recent advances in the understanding of their roles in orchestrating the response of the immune system to bacterial and fungal infection, and damaged self. We also discuss the identification and structure-activity relationships of activating ligands, particularly trehalose dimycolate (TDM and related mycobacterial glycolipids, which have significant potential in the development of TH1/TH17 vaccination strategies.

  7. Climate Change Education for General Education Faculty

    Science.gov (United States)

    Ozbay, G.; Fox-Lykens, R.; Fuoco, M. J.; Phalen, L.; Harcourt, P.; Veron, D. E.; Rogers, M.; Merrill, J.

    2016-12-01

    As MADE-CLEAR scientists, our ultimate goal is to inform the public about climate change through education. Education will provide citizens with important tools for adapting and coping against climate change through the understanding of the cause and effects of climate change, and the role they play in counteracting these effects. MADE-CLEAR is connecting educators with resources such as lesson plans and hands-on activities so they can easily incorporate climate change into their curriculum. This past year Delaware State University held workshops for Chemistry and Math faculty to provide information and resources to help integrate climate change education into their classes. We presented them with information on climate change and demonstrated several laboratory activities that would be applicable to their classes. Such activities included a sea level rise graphing exercise, ocean acidification pH demonstration, ocean acidification's effect on organism's demonstration, carbon dioxide variability and heat trapping gas simulation. The goals of the workshops are to implement a multidisciplinary approach in climate change education. Workshops are prepared hands-on heavy followed by the lectures and video resources. Pre- and post-workshop assessment questions on the workshop contents are provided to monitor faculty understanding of the climate change content. In doing so, we aim to improve climate literacy in our higher education students.

  8. BCL-xL/MCL-1 inhibition and RARγ antagonism work cooperatively in human HL60 leukemia cells.

    Science.gov (United States)

    Perri, Mariarita; Yap, Jeremy L; Yu, Jianshi; Cione, Erika; Fletcher, Steven; Kane, Maureen A

    2014-10-01

    The acute promyelocytic leukemia (APL) subtype of acute myeloid leukemia (AML) is characterized by chromosomal translocations that result in fusion proteins, including the promyelocytic leukemia-retinoic acid receptor, alpha fusion protein (PML-RARα). All-trans retinoic acid (atRA) treatment is the standard drug treatment for APL yielding cure rates > 80% by activating transcription and proteasomal degradation of retinoic acid receptor, alpha (RARα). Whereas combination therapy with As2O3 has increased survival further, patients that experience relapse and are refractory to atRA and/or As2O3 is a clinically significant problem. BCL-2 family proteins regulate apoptosis and over-expression of anti-apoptotic B-cell leukemia/lymphoma 2 (BCL-2) family proteins has been associated with chemotherapeutic resistance in APL including impairment of the ability of atRA to induce growth arrest and differentiation. Here we investigated the novel BH3 domain mimetic, JY-1-106, which antagonizes the anti-apoptotic BCL-2 family members B-cell lymphoma-extra large (BCL-xL) and myeloid cell leukemia-1 (MCL-1) alone and in combination with retinoids including atRA, AM580 (RARα agonist), and SR11253 (RARγ antagonist). JY-1-106 reduced cell viability in HL-60 cells alone and in combination with retinoids. The combination of JY-1-106 and SR11253 had the greatest impact on cell viability by stimulating apoptosis. These studies indicate that dual BCL-xL/MCL-1 inhibitors and retinoids could work cooperatively in leukemia treatment.

  9. Long-term follow-up of MCL patients treated with single-agent ibrutinib: updated safety and efficacy results

    Science.gov (United States)

    Blum, Kristie A.; Martin, Peter; Goy, Andre; Auer, Rebecca; Kahl, Brad S.; Jurczak, Wojciech; Advani, Ranjana H.; Romaguera, Jorge E.; Williams, Michael E.; Barrientos, Jacqueline C.; Chmielowska, Ewa; Radford, John; Stilgenbauer, Stephan; Dreyling, Martin; Jedrzejczak, Wieslaw Wiktor; Johnson, Peter; Spurgeon, Stephen E.; Zhang, Liang; Baher, Linda; Cheng, Mei; Lee, Dana; Beaupre, Darrin M.; Rule, Simon

    2015-01-01

    Ibrutinib, an oral inhibitor of Bruton tyrosine kinase, is approved for patients with mantle cell lymphoma (MCL) who have received one prior therapy. We report the updated safety and efficacy results from the multicenter, open-label phase 2 registration trial of ibrutinib (median 26.7-month follow-up). Patients (N = 111) received oral ibrutinib 560 mg once daily, and those with stable disease or better could enter a long-term extension study. The primary end point was overall response rate (ORR). The median patient age was 68 years (range, 40-84), with a median of 3 prior therapies (range, 1-5). The median treatment duration was 8.3 months; 46% of patients were treated for >12 months, and 22% were treated for ≥2 years. The ORR was 67% (23% complete response), with a median duration of response of 17.5 months. The 24-month progression-free survival and overall survival rates were 31% (95% confidence interval [CI], 22.3-40.4) and 47% (95% CI, 37.1-56.9), respectively. The most common adverse events (AEs) in >30% of patients included diarrhea (54%), fatigue (50%), nausea (33%), and dyspnea (32%). The most frequent grade ≥3 infections included pneumonia (8%), urinary tract infection (4%), and cellulitis (3%). Grade ≥3 bleeding events in ≥2% of patients were hematuria (2%) and subdural hematoma (2%). Common all-grade hematologic AEs were thrombocytopenia (22%), neutropenia (19%), and anemia (18%). The prevalence of infection, diarrhea, and bleeding was highest for the first 6 months of therapy and less thereafter. With longer follow-up, ibrutinib continues to demonstrate durable responses and favorable safety in relapsed/refractory MCL. The trial is registered to www.ClinicalTrials.gov as #NCT01236391. PMID:26059948

  10. Bax/Mcl-1 balance affects neutrophil survival in intermittent hypoxia and obstructive sleep apnea: effects of p38MAPK and ERK1/2 signaling

    Directory of Open Access Journals (Sweden)

    Dyugovskaya Larissa

    2012-10-01

    Full Text Available Abstract Background Prolonged neutrophil survival is evident in various cardiovascular and respiratory morbidities, in hypoxic conditions in-vitro and in patients with obstructive sleep apnea (OSA characterized by nightly intermittent hypoxia (IH. This may lead to persistent inflammation, tissue injury and dysfunction. We therefore investigated by a translational approach the potential contribution of the intrinsic stress-induced mitochondrial pathway in extending neutrophil survival under IH conditions. Thus, neutrophils of healthy individuals treated with IH in-vitro and neutrophils of OSA patients undergoing nightly IH episodes in-vivo were investigated. Specifically, the balance between pro-apoptotic Bax and anti-apoptotic Mcl-1 protein expression, and the potential involvement of p38MAPK and ERK1/2 signaling pathways in the control of Mcl-1 expression were investigated. Methods Purified neutrophils were exposed to IH and compared to normoxia and to sustained hypoxia (SH using a BioSpherix-OxyCycler C42 system. Bax and Mcl-1 levels, and p38MAPK and ERK1/2 phosphorylation were determined by western blotting. Also, Bax/Mcl-1 expression and Bax translocation to the mitochondria were assessed by confocal microscopy in pre-apoptotic neutrophils, before the appearance of apoptotic morphology. Co-localization of Bax and mitochondria was quantified by LSM 510 CarlZeiss MicroImaging using Manders Overlap Coefficient. A paired two-tailed t test, with Bonferroni correction for multiple comparisons, was used for statistical analysis. Results Compared to normoxia, IH and SH up-regulated the anti-apoptotic Mcl-1 by about 2-fold, down-regulated the pro-apoptotic Bax by 41% and 27%, respectively, and inhibited Bax co-localization with mitochondria before visible morphological signs of apoptosis were noted. IH induced ERK1/2 and p38MAPKs phosphorylation, whereas SH induced only p38MAPK phosphorylation. Accordingly, both ERK and p38MAPK inhibitors attenuated

  11. Exploring the Relationship between Faculty Concerns and Faculty Characteristics

    Science.gov (United States)

    Kim, Myoungsook; Cho, YoonJung; Svinicki, Marilla D.

    2011-01-01

    The purpose of this study is to explore how college faculty characteristics are related to their teaching concerns based on Fuller's model of teacher concern (self, task, and impact concern). Fuller's model was supported by self and task concerns, though impact concern did not follow the model. Impact concern was the highest among the three…

  12. Faculty and Technology: Implications for Faculty Training and Technology Leadership

    Science.gov (United States)

    Keengwe, Jared; Kidd, Terry; Kyei-Blankson, Lydia

    2009-01-01

    The purpose of this study was to explore the factors affecting ICT adoption process and the implications for faculty training and technology leadership. Respondents represented a wide range of academic and professional positions. They identified themselves as Assistant, Associate, and Professor as well as Instructional Designer, Director of…

  13. Faculty-Curriculum Development. Curriculum Design by Nursing Faculty.

    Science.gov (United States)

    Yura, Helen; And Others

    Faculty curriculum development, and specific applications to nursing education, are addressed in 37 papers and 6 discussion summaries from 1973 and 1974 workshops sponsored by the National League for Nursing. Attention is directed to: the curriculum development process, curriculum evaluation, the conceptual framework as a part of curriculum…

  14. Mutated Ptpn11 alters leukemic stem cell frequency and reduces the sensitivity of acute myeloid leukemia cells to Mcl1 inhibition.

    Science.gov (United States)

    Chen, L; Chen, W; Mysliwski, M; Serio, J; Ropa, J; Abulwerdi, F A; Chan, R J; Patel, J P; Tallman, M S; Paietta, E; Melnick, A; Levine, R L; Abdel-Wahab, O; Nikolovska-Coleska, Z; Muntean, A G

    2015-06-01

    PTPN11 encodes the Shp2 non-receptor protein-tyrosine phosphatase implicated in several signaling pathways. Activating mutations in Shp2 are commonly associated with juvenile myelomonocytic leukemia but are not as well defined in other neoplasms. Here we report that Shp2 mutations occur in human acute myeloid leukemia (AML) at a rate of 6.6% (6/91) in the ECOG E1900 data set. We examined the role of mutated Shp2 in leukemias harboring MLL translocations, which co-occur in human AML. The hyperactive Shp2E76K mutant, commonly observed in leukemia patients, significantly accelerated MLL-AF9-mediated leukemogenesis in vivo. Shp2E76K increased leukemic stem cell frequency and affords MLL-AF9 leukemic cells IL3 cytokine hypersensitivity. As Shp2 is reported to regulate anti-apoptotic genes, we investigated Bcl2, Bcl-xL and Mcl1 expression in MLL-AF9 leukemic cells with and without Shp2E76K. Although the Bcl2 family of genes was upregulated in Shp2E76K cells, Mcl1 showed the highest upregulation in MLL-AF9 cells in response to Shp2E76K. Indeed, expression of Mcl1 in MLL-AF9 cells phenocopies expression of Shp2E76K, suggesting Shp2 mutations cooperate through activation of anti-apoptotic genes. Finally, we show Shp2E76K mutations reduce sensitivity of AML cells to small-molecule-mediated Mcl1 inhibition, suggesting reduced efficacy of drugs targeting MCL1 in patients with hyperactive Shp2.

  15. Overexpression of Mcl-1 Confers Multidrug Resistance, Whereas Topoisomerase IIβ Downregulation Introduces Mitoxantrone-Specific Drug Resistance in Acute Myeloid Leukemia

    Science.gov (United States)

    Hermanson, David L.; Das, Sonia G.; Li, Yunfang

    2013-01-01

    Drug resistance is a serious challenge in cancer treatment and can be acquired through multiple mechanisms. These molecular changes may introduce varied extents of resistance to different therapies and need to be characterized for optimal therapy choice. A recently discovered small molecule, ethyl-2-amino-6-(3,5-dimethoxyphenyl)-4-(2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate) (CXL017), reveals selective cytotoxicity toward drug-resistant leukemia. A drug-resistant acute myeloid leukemia cell line, HL60/MX2, also failed to acquire resistance to CXL017 upon chronic exposure and regained sensitivity toward standard therapies. In this study, we investigated the mechanisms responsible for HL60/MX2 cells’ drug resistance and the molecular basis for its resensitization. Results show that the HL60/MX2 cell line has an elevated level of Mcl-1 protein relative to the parental cell line, HL60, and its resensitized cell line, HL60/MX2/CXL017, whereas it has a reduced level of topoisomerase IIβ. Mcl-1 overexpression in HL60/MX2 cells is mainly regulated through phospho-extracellular signal-regulated protein kinases 1 and 2–mediated Mcl-1 stabilization, whereas the reduction of topoisomerase IIβ in HL60/MX2 cells is controlled through genetic downregulation. Upregulating Mcl-1 introduces multidrug resistance to standard therapies, whereas its downregulation results in significant cell death. Downregulating topoisomerase IIβ confers resistance specifically to mitoxantrone, not to other topoisomerase II inhibitors. Overall, these data suggest that Mcl-1 overexpression is a critical determinant for cross-resistance to standard therapies, whereas topoisomerase IIβ downregulation is specific to mitoxantrone resistance. PMID:23696245

  16. Driving Success over the Past 50 Years-The Faculty in Academic Veterinary Medicine.

    Science.gov (United States)

    Buss, Daryl D

    2015-01-01

    The faculty at member schools and colleges of the Association of American Veterinary Medical Colleges (AAVMC) are critical for continued progress in veterinary medicine. The success of those faculty members over the past 50 years has positioned veterinary medicine to engage an ever-widening array of opportunities, responsibilities, and societal needs. Yet the array of skills and accomplishments of faculty in academic veterinary medicine are not always visible to the public, or even within our profession. The quality and the wide range of their scholarship are reflected, in part, through the according of national and international awards and honors from organizations relevant to their particular areas of expertise. The goal of this study was to illustrate the breadth of expertise and the quality of the faculty at 34 schools/colleges of veterinary medicine by examining the diversity of organizations that have recognized excellence in faculty achievements through a variety of awards.

  17. Private Cloud Communities for Faculty and Students

    Directory of Open Access Journals (Sweden)

    Daniel R. Tomal

    2015-09-01

    Full Text Available Massive open online courses (MOOCs and public and private cloud communities continue to flourish in the field of higher education. However, MOOCs have received criticism in recent years and offer little benefit to students already enrolled at an institution. This article advocates for the collaborative creation and use of institutional, program or student-specific private cloud communities developed as a way to promote academic identity, information dissemination, social discourse, and to form a bridge between faculty, administration and students. Concrete steps to build a private cloud are described. Placing a greater emphasis on meeting the needs of enrolled students versus engaging the masses in a MOOC for “edutainment” purposes is recommended.

  18. Education: Chemistry Faculty Job Mobility Surveyed.

    Science.gov (United States)

    Chemical and Engineering News, 1981

    1981-01-01

    Summarizes results of a survey undertaking to describe the extent of movement of chemistry faculty members (N=1207) from academic to industrial positions. Numbers of male and female faculty within categories of reasons for leaving are also reported. (CS)

  19. Faculty Changing Departments: Why, Who, and When?

    Science.gov (United States)

    Wallerstein, George

    1976-01-01

    Departmental changes by faculty staff are suggested to be one solution to the budgetary problems of some institutions, rather than the more prevalent practice of nonreappointment of untenured faculty. (LBH)

  20. Nursing faculty preparedness for clinical teaching.

    Science.gov (United States)

    Suplee, Patricia Dunphy; Gardner, Marcia; Jerome-D'Emilia, Bonnie

    2014-03-01

    Nursing faculty who teach in clinical settings face complex situations requiring evidence-based educational and evaluative strategies, yet many have had limited preparation for these tasks. A convenience sample of 74 nursing faculty participated in a survey about clinical teaching in prelicensure nursing programs. Most faculty developed teaching skills through conferences (57%), orientation at their educational institution (53%), or exposure in graduate school (38%). Thirty-one percent reported having no preparation for clinical teaching. Faculty felt least prepared to manage students with learning, physical, or emotional disabilities and incivility. Twenty-six percent had no preparation for evaluating students in the clinical setting, and only 17% had worked with a faculty mentor. Few evidence-based teaching strategies were used by the faculty. These findings indicate gaps exist in the preparation of clinical faculty. Graduate education, comprehensive orientation programs, and continuing professional development may help to ensure faculty are effective in managing and evaluating student learning. Copyright 2014, SLACK Incorporated.

  1. The Role of Transformational Leadership, Organizational Culture and Organizational Learning in Improving the Performance of Iranian Agricultural Faculties

    Science.gov (United States)

    Abbasi, Enayat; Zamani-Miandashti, Naser

    2013-01-01

    This empirical research was conducted to investigate the role of transformational leadership, organizational culture and organizational learning in improving the performance of Iranian agricultural faculties and leading them to become learning organizations. The research population consisted of all faculty members of public agricultural faculties…

  2. A Case for Competencies: Assessing the Value of Trait-Based Performance Appraisal for Non-Faculty University Employees

    Science.gov (United States)

    Messa, Emily A.; Horn, Catherine L.; Longacre, Teri Elkins; Olenchak, F. Richard; Penney, Lisa M.

    2016-01-01

    A case study analyzed how supervisors at one university selected competencies, or trait-based skills, for non-faculty employees. This case study provides a valuable contribution by focusing on employees at one institution type--a large, public research university. While it has been documented that non-faculty employees provide important…

  3. The Role of Transformational Leadership, Organizational Culture and Organizational Learning in Improving the Performance of Iranian Agricultural Faculties

    Science.gov (United States)

    Abbasi, Enayat; Zamani-Miandashti, Naser

    2013-01-01

    This empirical research was conducted to investigate the role of transformational leadership, organizational culture and organizational learning in improving the performance of Iranian agricultural faculties and leading them to become learning organizations. The research population consisted of all faculty members of public agricultural faculties…

  4. Social Regulation and Shifting Institutional Culture in Higher Education: A Reflective Account of a Faculty of Education

    Science.gov (United States)

    Small, R.; Smith, J.; Williams, C.; Fataar, A.

    2011-01-01

    This article addresses the question: To what extent did social regulation impact on the institutional culture of a faculty of education at a South African public higher education institution over a ten year period from approximately 1998 until 2007? We engaged in systematic reflections about our experiences as members of the Faculty of Education,…

  5. Research Self-Efficacy Sources and Research Motivation in a Foreign Language University Faculty in Mexico: Implications for Educational Policy

    Science.gov (United States)

    Reyes-Cruz, María del Rosario; Perales-Escudero, Moisés Damián

    2016-01-01

    The research self-efficacy and motivation of foreign language (FL) faculty in periphery countries is under-researched, yet there is a need to understand the impact of public policies that drive such faculty to conduct research. This paper reports a qualitative case study investigating research self-efficacy and research motivation in a group of…

  6. Faculty Satisfaction in Academic Medicine.

    Science.gov (United States)

    Nyquist, Julie G.; Hitchcock, Maurice A.; Teherani, Arianne

    2000-01-01

    Describes the challenges and elements of satisfaction in academic medicine. Proposes a model of academic faculty satisfaction which postulates that organizational, job-related, and personal factors combine to develop self-knowledge, social knowledge, and satisfaction with outcomes of productivity, retention, and learner-patient satisfaction. (DB)

  7. Faculty Organizational Commitment and Citizenship

    Science.gov (United States)

    Lawrence, Janet; Ott, Molly; Bell, Alli

    2012-01-01

    Building on a theoretical framework that links characteristics of individuals and their work settings to organizational commitment (OC) and citizenship behavior, this study considers why faculty may be disengaging from institutional service. Analyses of survey data collected from a state system of higher education suggest that job characteristics,…

  8. Faculty Adoption of Educational Technology

    Science.gov (United States)

    Moser, Franziska Zellweger

    2007-01-01

    Although faculty support has been identified as a critical factor in the success of educational-technology programs, many people involved in such efforts underestimate the complexities of integrating technology into teaching. In this article, the author proposes an adoption cycle to help tackle the complex issue of technology adoption for…

  9. Faculty Preceptions of Learning Spaces

    Science.gov (United States)

    Friskney, Doyle

    2014-01-01

    The project researched faculty perceptions of learning spaces and their possible impact on student persistence at two community colleges in Kentucky. The researchers found through literature review, surveys, and interviews that learning spaces that enhanced student engagement and collaboration could positively impact student persistence. The…

  10. Faculty Perceptions of Learning Spaces

    Science.gov (United States)

    Czarapata, Paul B.

    2014-01-01

    This project researched faculty perceptions of learning spaces and their possible impact on student persistence at two community colleges in Kentucky. The researchers found through literature review, surveys, and interviews that learning spaces that enhanced student engagement and collaboration could positively impact student persistence. The…

  11. Teaching portfolios for faculty evaluation.

    Science.gov (United States)

    Melland, H I; Volden, C M

    1996-01-01

    Teaching portfolios are stimulating much discussion as more comprehensive approaches to evaluating teaching are sought. Portfolios can be used effectively for both formative and summative evaluative purposes. The content of a portfolio may vary greatly, but commonly includes material that reflects student learning, evaluative materials, and a personal statement on the faculty's philosophy of education. Professional growth often accompanies developing a personal teaching portfolio.

  12. Electronic Portfolios for Faculty Development.

    Science.gov (United States)

    Heath, Marilyn; Cockerham, Steve

    This paper examines the conceptual process of creating an electronic professional portfolio for faculty development. The characteristics of electronic professional portfolios and the benefits of electronic portfolio development are discussed. Additional topics covered include: collection and selection of portfolio contents; reflection on portfolio…

  13. Paperless Grades and Faculty Development.

    Science.gov (United States)

    Hardy, James C.; Jones, Dennis; Turner, Sandy

    2003-01-01

    Provides overview of process of switching from paper-based grade reporting to computer-based grading. Authors found that paperless grading decreased number of errors, made student access more immediate, and reduced costs incurred by purchasing and storing grade-scanning sheets. Authors also argue that direct entry grading encourages faculty to…

  14. Faculty Workload: An Analytical Approach

    Science.gov (United States)

    Dennison, George M.

    2012-01-01

    Recent discussions of practices in higher education have tended toward muck-raking and self-styled exposure of cynical self-indulgence by faculty and administrators at the expense of students and their families, as usually occurs during periods of economic duress, rather than toward analytical studies designed to foster understanding This article…

  15. Faculty Perceptions of Learning Spaces

    Science.gov (United States)

    Czarapata, Paul B.

    2014-01-01

    This project researched faculty perceptions of learning spaces and their possible impact on student persistence at two community colleges in Kentucky. The researchers found through literature review, surveys, and interviews that learning spaces that enhanced student engagement and collaboration could positively impact student persistence. The…

  16. Faculty Preceptions of Learning Spaces

    Science.gov (United States)

    Friskney, Doyle

    2014-01-01

    The project researched faculty perceptions of learning spaces and their possible impact on student persistence at two community colleges in Kentucky. The researchers found through literature review, surveys, and interviews that learning spaces that enhanced student engagement and collaboration could positively impact student persistence. The…

  17. Faculty Development: Assessing Learner Achievement.

    Science.gov (United States)

    Frey, Barbara A.; Overfield, Karen

    This study addressed the challenges of developing a faculty professional development workshop on assessment, measurement, and evaluation of achievement in adult learners. The setting for the workshop was a system of postsecondary career colleges throughout the United States. The curriculum development model of D. Kirkpatrick (1994) was used as a…

  18. Embedded Neoliberalism within Faculty Behaviors

    Science.gov (United States)

    Levin, John S.; Aliyeva, Aida

    2015-01-01

    Although there are claims that neoliberalism has not only commandeered the agenda and actions of universities and colleges but also become identified with the work of academic professionals, there is little empirical evidence to show that neoliberalism has infiltrated the work of faculty. This qualitative field work investigation of three…

  19. 菲律宾蛤仔凝集素抑菌机制的研究%Antibacterial mechanism of short-neck clam Ruditapes philippinarum lectin(MCL-T)

    Institute of Scientific and Technical Information of China (English)

    佟长青; 李琦; 曲敏; 金桥; 李伟

    2014-01-01

    The effects of short-neck clam Ruditapes philippinarum lectin( MCL-T) on respiration, and synthesis of total DNA, total RNA and soluble proteins in bacteria Staphylococcus aureus and Bacillus subtilis, and reaction of MCL-T with out membrane proteins ( OMP) of S. aureus and RNase activity of S. aureus were studied by solid phase assay to elucidate antibacterial mechanism of MCL-T. The MCL-T was shown to inhibit pentose phosphate pathway ( HMP) in respiratory metabolism of the bacteria. The contents of total DNA, total RNA and soluble pro-teins were decreased in both bacteria incubated with MCL-T with increase in concentrations of MCL-T. The solid phase assay revealed that MCL-T binding to out membrane proteins ( OMP) was concentration-dependent and in-hibited by PSM or GalNAc with RNase activity. The findings indicated that MCL-T inhibited the growth of the two bacteria by respiration inhibition, and the antibacterial activities of MCL-T were related to its domains of binding to OMP and to RNase activity.%通过分析菲律宾蛤仔Ruditapes philippinarum凝集素( MCL-T)对细菌的呼吸代谢及细菌中总DNA、总RNA和可溶性蛋白合成的影响,以及MCL-T与金黄色葡萄球菌膜蛋白( OMP)的相互作用及其核酸酶( RNase)活性,研究了MCL-T的抑菌机制。结果表明: MCL-T具有抑制金黄色葡萄球菌Staphylococcus au-reus和枯草芽孢杆菌Bacillus subtilis代谢过程中的磷酸戊糖途径( HMP); MCL-T与金黄色葡萄球菌或枯草芽孢杆菌共同培养时,随着MCL-T质量浓度的增加,金黄色葡萄球菌或枯草芽孢杆菌中的总 DNA、总RNA和可溶性蛋白含量均呈下降趋势;固相吸附测试表明, MCL-T可与金黄色葡萄球菌膜蛋白结合,两者的结合具有浓度依赖关系,该结合位点受N-乙酰-D-半乳糖酰胺( N-acetyl-D-galactosamine, GalNAc)及猪胃黏蛋白(Mucin from porcine stomach, PSM)的抑制, MCL-T具有RNase活性。研究表明, MCL-T通过抑制细菌的呼吸代

  20. Relationships between teaching faculty and teaching librarians

    CERN Document Server

    Katz, Linda S

    2014-01-01

    Every librarian who teaches in an academic library setting understands the complexities involved in partnering with teaching faculty. Relationships Between Teaching Faculty and Teaching Librarians recounts the efforts of librarians and faculty working together in disciplines across the board to create and sustain connections crucial to the success of library instruction. This unique collection of essays examines various types of partnerships between librarians and faculty (networking, coordination, and collaboration) and addresses the big issues involved, including teaching within an academic

  1. Faculty Learning Matters: Organizational Conditions and Contexts That Shape Faculty Learning

    Science.gov (United States)

    O'Meara, KerryAnn; Rivera, Mark; Kuvaeva, Alexandra; Corrigan, Kristen

    2017-01-01

    This study explored the relationships between faculty scholarly learning, faculty teaching learning, institutional support, faculty demographics, disciplinary groups, working conditions, and career outcomes such as retention, productivity, satisfaction, and career agency. We found that the stronger the scholarly learning faculty members reported,…

  2. Faculty attitudes about interprofessional education

    Directory of Open Access Journals (Sweden)

    Gary L. Beck Dallaghan

    2016-06-01

    Full Text Available Background: Interprofessional education (IPE is an important component to training health care professionals. Research is limited in exploring the attitudes that faculty hold regarding IPE and what barriers they perceive to participating in IPE. The purpose of this study was to identify faculty attitudes about IPE and to identify barriers to participating in campus-wide IPE activities. Methods: A locally used questionnaire called the Nebraska Interprofessional Education Attitudes Scale (NIPEAS was used to assess attitudes related to interprofessional collaboration. Questions regarding perceived barriers were included at the end of the questionnaire. Descriptive and non-parametric statistics were used to analyze the results in aggregate as well as by college. In addition, open-ended questions were analyzed using an immersion/crystallization framework to identify themes. Results: The results showed that faculty had positive attitudes of IPE, indicating that is not a barrier to participating in IPE activities. Most common barriers to participation were scheduling conflicts (x24,285=19.17, p=0.001, lack of department support (4,285=10.09, p=0.039, and lack of awareness of events (x24,285=26.38, p=0.000. Narrative comments corroborated that scheduling conflicts are an issue because of other priorities. Those who commented also added to the list of barriers, including relevance of the activities, location, and prior negative experiences. Discussion: With faculty attitudes being positive, the exploration of faculty's perceived barriers to IPE was considered even more important. Identifying these barriers will allow us to modify our IPE activities from large, campus-wide events to smaller activities that are longitudinal in nature, embedded within current curriculum and involving more authentic experiences.

  3. New England Faculty and College Students Differ in Their Views About Evolution, Creationism, Intelligent Design, and Religiosity.

    Science.gov (United States)

    Paz-Y-Miño C, Guillermo; Espinosa, Avelina

    2011-06-01

    Public acceptance of evolution in Northeastern U.S. is the highest nationwide, only 59%. Here, we compare perspectives about evolution, creationism, intelligent design (ID), and religiosity between highly educated New England faculty (n=244; 90% Ph.D. holders in 40 disciplines at 35 colleges/universities) and college students from public secular (n=161), private secular (n=298), and religious (n=185) institutions: 94/3% of the faculty vs. 64/14% of the students admitted to accepting evolution openly and/or privately, and 82/18% of the faculty vs. 58/42% of the students thought that evolution is definitely true or probably true, respectively. Only 3% of the faculty vs. 23% of the students thought that evolution and creationism are in harmony. Although 92% of faculty and students thought that evolution relies on common ancestry, one in every four faculty and one in every three students did not know that humans are apes; 15% of the faculty vs. 34% of the students believed, incorrectly, that the origin of the human mind cannot be explained by evolution, and 30% of the faculty vs. 72% of the students was Lamarckian (believed in inheritance of acquired traits). Notably, 91% of the faculty was very concerned (64%) or somehow concerned (27%) about the controversy evolution vs creationism vs ID and its implications for science education: 96% of the faculty vs. 72% of the students supported the exclusive teaching of evolution while 4% of the faculty vs. 28% of the students favored equal time to evolution, creationism and ID; 92% of the faculty vs. 52% of the students perceived ID as not scientific and proposed to counter evolution or as doctrine consistent with creationism. Although ≈30% of both faculty and students considered religion to be very important in their lives, and ≈20% admitted to praying daily, the faculty was less religious (Religiosity Index faculty=0.5 and students=0.75) and, as expected, more knowledgeable about science (Science Index faculty=2.27 and

  4. Faculty Recruitment in an Era of Change

    Science.gov (United States)

    Levine, Marilyn; Schimpf, Martin

    2010-01-01

    Faculty recruitment is a challenge for administration and departments, especially in an era of change in the academy. This article builds on information from an interactive conference panel session that focused on faculty recruitment best practices. The article addresses faculty recruitment strategies that focus on the optimization of search…

  5. Retrenchment Clauses in Faculty Union Contracts.

    Science.gov (United States)

    Rhoades, Gary

    1993-01-01

    Examination of retrenchment clauses in the faculty union contracts at 42 colleges and universities focused on implications for tenure rights and the roles prescribed for faculty and administrators. Concepts of financial exigency and shared governance are highlighted. Contracts were found to provide faculty with a limited and reactive role during…

  6. A Faculty Code is not a Coda

    Science.gov (United States)

    O'Neil, Robert M.

    1974-01-01

    Many college and university faculties have adopted codes of faculty responsibilities and self-regulation. Firsthand advice on creating a code precedes an example of one: the new University of California Policy on Faculty Conduct and the Administration of Discipline. (Editor/PG)

  7. Development of New Faculty in Higher Education.

    Science.gov (United States)

    Boyden, Kathleen M.

    2000-01-01

    Nursing faculty are challenged by changing expectations of undergraduate education, decreasing resources, and widespread technology use. Stressors on new faculty include time, lack of peer support, inadequate feedback, and family-work imbalance. Suggestions for new faculty development include orientation, mentoring, and strategic planning for…

  8. Perceptions of Faculty Status among Academic Librarians

    Science.gov (United States)

    Galbraith, Quinn; Garrison, Melissa; Hales, Whitney

    2016-01-01

    This study measures the opinions of ARL librarians concerning the benefits and disadvantages of faculty status in academic librarianship. Average responses from faculty and nonfaculty librarians, as well as from tenured and tenure-track librarians, are analyzed to determine the general perceptions of each group. Overall, faculty librarians…

  9. Faculty Senates and the Fiscal Crisis.

    Science.gov (United States)

    Ridgely, Julia

    1993-01-01

    A 1993 conference of college faculty senate members is discussed, focusing on common difficulties in governance during a period of retrenchment and due to the faculty culture. Some recent research into the relationship of finances to academic freedom and tenure is examined. Strategies for enhancing faculty senate effectiveness are suggested. (MSE)

  10. Undergraduate Nursing Student Experiences with Faculty Bullying

    Science.gov (United States)

    Mott, Jason D.

    2013-01-01

    Incivility and bullying in nursing education has become an area of increased interest. Incivility literature has focused primarily on student-to-faculty incivility. Less focus has been placed on faculty-to-student bullying. This study examined the lived experiences of undergraduate nursing students with faculty bullying. Using descriptive…

  11. Special Education Faculty Needs Assessment Study Implications

    Science.gov (United States)

    Smith, D.D.; Tyler, N.; Montrosse, B.E.; Young, C.; Robb, S.M.

    2011-01-01

    This paper presents the highlights of the Special Education Faculty Needs Assessment Study (SEFNA). Actions taken after the release of The 2001 Faculty Shortage Study demonstrate that supply-and-demand imbalances can be improved. The projected shortage of special education faculty will directly and negatively affect students with disabilities and…

  12. Faculty and Technology: Implications for Faculty Training and Technology Leadership

    Science.gov (United States)

    Keengwe, Jared; Kidd, Terry; Kyei-Blankson, Lydia

    2009-02-01

    The purpose of this study was to explore the factors affecting ICT adoption process and the implications for faculty training and technology leadership. Respondents represented a wide range of academic and professional positions. They identified themselves as Assistant, Associate, and Professor as well as Instructional Designer, Director of Technology, Information Manager, eLearning Manager, Assistant Department Chair, Associate Vice President of Academic Affairs and Consultant. The respondents identified Organizational Support, Leadership, Training and Development, and Resources as the predominate themes affecting Information and Communication Technology (ICT) adoption process in higher education. Evidence from this study offers insights on how higher education administrators and technology leaders could help their faculty and staff to implement appropriate ICT tools and practices to improve student learning.

  13. Database Support for Research in Public Administration

    Science.gov (United States)

    Tucker, James Cory

    2005-01-01

    This study examines the extent to which databases support student and faculty research in the area of public administration. A list of journals in public administration, public policy, political science, public budgeting and finance, and other related areas was compared to the journal content list of six business databases. These databases…

  14. Analysis of publications by the State University of Londrina nursing faculty, from 1995 to 2006 Análise das publicações dos enfermeiros docentes da Universidade Estadual de Londrina-PR de 1995 a 2006

    Directory of Open Access Journals (Sweden)

    Maria Helena Dantas de Menezes Guariente

    2009-08-01

    Full Text Available The objective of this research was to analyze and classify the prevailing themes and ways of research used in publications made in the years of 1995 to 2006 by teachers from the Nursing Department in the State University of Londrina. The place and time of formation, titles, the field of work of the teachers and the classification of the periodicals were also analyzed. Criteria from QUALIS/CAPES – the national program for the qualification of higher education professionals, were used for the classification of the publications. The data was collected from Curricula Lattes where there was a data base of 183 publications edited in 51 periodicals, out of those, 56,9% were in the theme of organization, 31,14% in the field of assistance and 12,02% with the profession theme. As far as classification of the QUALIS/CAPES is concerned, out of 51 means of publication, 33 of them fit into categories A to C, most of them published at the national level only. The analysis revealed that the predominance of teachers in the nursing department at UEL have an investigative profile in the organizational field and also suggested future researches in less explored fields in order to strengthen this professional group. O objetivo desse estudo foi analisar e classificar a direcionalidade temática e as linhas de pesquisa das publicações feitas pelos docentes do curso de enfermagem da Universidade Estadual de Londrina-PR (UEL no período de 1995 a 2006. Também foram analisados o local e o tempo de formação, a titulação, a área de atuação dos docentes e a classificação dos periódicos. Como critérios balisadores da classificação dos artigos, adotou-se o QUALIS da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES. Os dados foram coletados na plataforma do Curriculum Lattes de cada docente, nos quais se levantou o registro de 183 publicações, editadas em 51 periódicos, sendo 56,9% na temática organizacional, 31,14% ao segmento

  15. Knowledge of journal impact factors among nursing faculty: a cross-sectional study

    Directory of Open Access Journals (Sweden)

    Maha Kumaran, MA, MLIS

    2017-04-01

    Conclusions: There are various reasons for choosing a journal for publication. It is important for librarians to understand faculty views of JIFs and their criteria for choosing journals for publication, so that librarians are better equipped to guide researchers in considering their academic goals, needs, and personal values.

  16. Role model behaviors of nursing faculty members in Thailand.

    Science.gov (United States)

    Klunklin, Areewan; Sawasdisingha, Piyawan; Viseskul, Nongkran; Funashima, Naomi; Kameoka, Tomomi; Nomoto, Yuriko; Nakayama, Toshiko

    2011-03-01

    Being a role model is very important in order for nurse teachers to promote students' competence and confidence. This descriptive study aimed at exploring the role model behavior of nursing faculty members in Thailand. The Self-Evaluation Scale on Role Model Behaviors for Nursing Faculty (Thai version) was used to collect data from 320 nursing faculty members in eight schools of nursing, four university nursing schools, one college under the Ministry of Public Health, one under the Bangkok Metropolitan Administration, and two private schools of nursing. The results revealed that the mean score of the overall items in the role model behaviors of nursing faculty members in Thailand, as perceived by themselves, was at a high level. The scores on each subscale of the role model behaviors also were high and related to respect for students, enthusiastic and high-quality teaching activities, showing the value of nursing practice and the nursing profession, social appropriateness, and ongoing professional development. The results can be used to further develop nurse professionals and to improve the effectiveness of clinical teaching in Thailand.

  17. Toward Inclusive STEM Classrooms: What Personal Role Do Faculty Play?

    Science.gov (United States)

    Killpack, Tess L; Melón, Laverne C

    2016-01-01

    Private and public policies are increasingly aimed at supporting efforts to broaden participation of a diverse body of students in higher education. Unfortunately, this increase in student diversity does not always occur alongside changes in institutional culture. Unexamined biases in institutional culture can prevent diverse students from thriving and persisting in science, technology, engineering, and mathematics (STEM) fields. Given the daily personal interactions that faculty have with students, we suggest that individual educators have the opportunity, and responsibility, to improve the retention and persistence of diverse students. However, in our experience, faculty professional development programs often limit discussions of diversity to "comfortable" topics (such as learning styles) and miss opportunities to explore deeper issues related to faculty privilege, implicit bias, and cues for stereotype threat that we all bring to the classroom. In this essay, we present a set of social science concepts that we can extend to our STEM courses to inform our efforts at inclusive excellence. We have recommended strategies for meaningful reflection and professional development with respect to diversity and inclusion, and aim to empower faculty to be change agents in their classrooms as a means to broadening participation in STEM fields.

  18. Toward Inclusive STEM Classrooms: What Personal Role Do Faculty Play?

    Science.gov (United States)

    Killpack, Tess L.; Melón, Laverne C.

    2016-01-01

    Private and public policies are increasingly aimed at supporting efforts to broaden participation of a diverse body of students in higher education. Unfortunately, this increase in student diversity does not always occur alongside changes in institutional culture. Unexamined biases in institutional culture can prevent diverse students from thriving and persisting in science, technology, engineering, and mathematics (STEM) fields. Given the daily personal interactions that faculty have with students, we suggest that individual educators have the opportunity, and responsibility, to improve the retention and persistence of diverse students. However, in our experience, faculty professional development programs often limit discussions of diversity to “comfortable” topics (such as learning styles) and miss opportunities to explore deeper issues related to faculty privilege, implicit bias, and cues for stereotype threat that we all bring to the classroom. In this essay, we present a set of social science concepts that we can extend to our STEM courses to inform our efforts at inclusive excellence. We have recommended strategies for meaningful reflection and professional development with respect to diversity and inclusion, and aim to empower faculty to be change agents in their classrooms as a means to broadening participation in STEM fields. PMID:27496362

  19. Knowledge of journal impact factors among nursing faculty: a cross-sectional study

    Science.gov (United States)

    Kumaran, Maha; Ha, Chau

    2017-01-01

    Objective The research assessed nursing faculty awareness and knowledge of the journal impact factor (JIF) and its impact on their publication choices. Methods A qualitative cross-sectional questionnaire was developed using Fluid Survey and distributed electronically to nursing faculty and instructors at three post-secondary institutions in Saskatchewan. Data were collected on place and status of employment, knowledge and awareness of JIFs, and criteria used to choose journals for publication. Results A total of forty-four nursing faculty and instructors completed the questionnaire. The authors found that faculty lack awareness or complete understanding of JIFs and that JIFs are not the most important or only criterion used when they choose a journal for publication. Conclusions There are various reasons for choosing a journal for publication. It is important for librarians to understand faculty views of JIFs and their criteria for choosing journals for publication, so that librarians are better equipped to guide researchers in considering their academic goals, needs, and personal values. PMID:28377676

  20. A Glance at the Faculty Employment System in American Public Universities --A Case Study of University of Minnesota%管窥美国公立大学的教师聘任制度——以明尼苏达大学为例

    Institute of Scientific and Technical Information of China (English)

    司晓宏

    2011-01-01

    明尼苏达大学是美国一所典型的公立大学,其教师聘任工作中的基本过程和做法,代表了美国大学教师聘任制度的主流。明大面向海内外公开招聘教师、终身教授晋升中非升即走、教师进退升降中学术权力为主导、教师管理工作的低重心等特征,深值我国大学在构建现代大学制度中予以借鉴。%The University of Minnesota is a typical public university in the United States. The principal process and methods in the course of faculty employment represent the main current. To set up modern university system in China, it is worthy of learning from the following American characteristics: advertising for teachers from home and abroad, the way of "to promote or to leave" in tenured professor promotion, academic authority predominance in deciding teachers' ascending or descending and the low center in teacher management.

  1. New England Faculty and College Students Differ in Their Views About Evolution, Creationism, Intelligent Design, and Religiosity

    OpenAIRE

    Paz-y-Miño C., Guillermo; Espinosa, Avelina

    2010-01-01

    Public acceptance of evolution in Northeastern U.S. is the highest nationwide, only 59%. Here, we compare perspectives about evolution, creationism, intelligent design (ID), and religiosity between highly educated New England faculty (n=244; 90% Ph.D. holders in 40 disciplines at 35 colleges/universities) and college students from public secular (n=161), private secular (n=298), and religious (n=185) institutions: 94/3% of the faculty vs. 64/14% of the students admitted to accepting evolution...

  2. PI3K and Bcl-2 inhibition primes glioblastoma cells to apoptosis through downregulation of Mcl-1 and Phospho-BAD.

    Science.gov (United States)

    Pareja, Fresia; Macleod, David; Shu, Chang; Crary, John F; Canoll, Peter D; Ross, Alonzo H; Siegelin, Markus D

    2014-07-01

    Glioblastoma multiforme (GBM) is a highly malignant human brain neoplasm with limited therapeutic options. GBMs display a deregulated apoptotic pathway with high levels of the antiapoptotic Bcl-2 family of proteins and overt activity of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. Therefore, combined interference of the PI3K pathway and the Bcl-2 family of proteins is a reasonable therapeutic strategy. ABT-263 (Navitoclax), an orally available small-molecule Bcl-2 inhibitor, and GDC-0941, a PI3K inhibitor, were used to treat established glioblastoma and glioblastoma neurosphere cells, alone or in combination. Although GDC-0941 alone had a modest effect on cell viability, treatment with ABT-263 displayed a marked reduction of cell viability and induction of apoptotic cell death. Moreover, combinatorial therapy using ABT-263 and GDC-0941 showed an enhanced effect, with a further decrease in cellular viability. Furthermore, combination treatment abrogated the ability of stem cell-like glioma cells to form neurospheres. ABT-263 and GDC-0941, in combination, resulted in a consistent and significant increase of Annexin V positive cells and loss of mitochondrial membrane potential compared with either monotherapy. The combination treatment led to enhanced cleavage of both initiator and effector caspases. Mechanistically, GDC-0941 depleted pAKT (Serine 473) levels and suppressed Mcl-1 protein levels, lowering the threshold for the cytotoxic actions of ABT-263. GDC-0941 decreased Mcl-1 in a posttranslational manner and significantly decreased the half-life of Mcl-1 protein. Ectopic expression of human Mcl-1 mitigated apoptotic cell death induced by the drug combination. Furthermore, GDC-0941 modulated the phosphorylation status of BAD, thereby further enhancing ABT-263-mediated cell death. Combination therapy with ABT-263 and GDC-0941 has novel therapeutic potential by specifically targeting aberrantly active, deregulated pathways in GBM, overcoming

  3. ESMD Space Grant Faculty Report

    Science.gov (United States)

    Guo, Jiang; Whitmore, Stephen; Radcliff, Roger; Misra, Prabhakar; Prasad, Nadipuram; Conrad, James; Lackey, Ellen; Selby, Gregory; Wersinger, Jean-Marie; Lambright, Jonathan

    2008-01-01

    The strength of the Exploration Systems Mission Directorate ESMD Faculty Project lies in its ability to meet National Aeronautics Space Administration NASA's Strategic Educational Outcome 1 by developing a sustainable and long-term integration of student involvement at academic institutions with all NASA Centers. This outcome is achieved by a three-fold approach: 1) by collecting Senior Design projects pertaining to Constellation work performed at each of the ten NASA Centers, 2) by engaging students at Minority Serving Institutions in the art of systems engineering and systems design of technologies required for space exploration, and 3) by identifying potential internships at each Center relative to exploration that provide students who are supported by their institutional Space Grant to engage in on-going mission-level and explorative systems designs. The objectives of the ESMD Faculty Project are to: 1. Aid the Centers (both Education Offices and associated technical organizations) in providing relevant opportunities for the ESMD Space Grant Program to support student and faculty in Senior Design projects 2. Enable better matches between the ESMD work required and what the Space Grant Consortia can do to effectively contribute to NASA programs 3. Provide the Space Grant Consortia an opportunity to strengthen relations with the NASA Centers 4. Develop better collective understanding of the U.S. Space Exploration Policy by the Center, Space Grant, faculty, Education Office, and students 5. Enable Space Grant institution faculty to better prepare their students to meet current and future NASA needs 6. Enable the Center Education Offices to strengthen their ties to their technical organizations and Space Grant Consortia 7. Aid KSC in gaining a greater and more detailed understanding of each of the Center activities Senior Design projects are intended to stimulate undergraduate students on current NASA activities related to lunar, Mars, and other planetary missions

  4. Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

    Directory of Open Access Journals (Sweden)

    A.M. Gumel

    2014-06-01

    Full Text Available Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1 and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w and PHA yields ranging from 10.12 g L-1 to 15.45 g L-1, respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7 monomer was also detected when C18:1 was fed. Polymer showed melting temperature (Tm of 42.0 (± 0.2 °C, glass transition temperature (Tg of -1.0 (± 0.2 °C and endothermic melting enthalpy of fusion (ΔHf of 110.3 (± 0.1 J g-1. The molecular weight (Mw range of the polymer was relatively narrow between 55 to 77 kDa.

  5. Metformin combined with sodium dichloroacetate promotes B leukemic cell death by suppressing anti-apoptotic protein Mcl-1.

    Science.gov (United States)

    Voltan, Rebecca; Rimondi, Erika; Melloni, Elisabetta; Gilli, Paola; Bertolasi, Valerio; Casciano, Fabio; Rigolin, Gian Matteo; Zauli, Giorgio; Secchiero, Paola

    2016-04-05

    Metformin and the mitochondrial targeting dichloroacetate (DCA) have recently received attention due to their ability to inhibit anaerobic glycolysis, which renders most cancer cells resistant to apoptosis induction. We observed that Metformin alone exhibited a dose-dependent anti-leukemic activity in both B leukemic cell lines and primary B-chronic lymphocytic leukemia (B-CLL) patients' cells and its anti-leukemic activity was enhanced when used in combination with DCA. In order to overcome the problems of poor bioavailability and cellular uptake, which limit DCA efficacy, we have designed and synthetized cocrystals consisting of Metformin and DCA (Met-DCA) at different stoichiometric ratios. Of note, the MetH(2)(++)•2DCA(-) cocrystal exhibited enhanced in vitro anti-leukemic activity, with respect to the treatment with the mix consisting of Metformin plus DCA. In particular, the treatment with the cocrystal MetH(2)(++)•2DCA(-) induced a synergistic apoptotic cell death coupled to a marked down-modulation of the anti-apoptotic Mcl-1 protein. Taken together, our data emphasize that innovative compounds based on Metformin-DCA combination merit to be further evaluated as chemotherapeutic agents for the treatment of B-CLL.

  6. miR-181b increases drug sensitivity in acute myeloid leukemia via targeting HMGB1 and Mcl-1.

    Science.gov (United States)

    Lu, Fei; Zhang, Jingru; Ji, Min; Li, Peng; Du, Yahui; Wang, Hongchun; Zang, Shaolei; Ma, Daoxin; Sun, Xiulian; Ji, Chunyan

    2014-07-01

    Multidrug resistance (MDR) remains the major cause of disease relapse and poor prognosis in adults with acute myeloid leukemia (AML). Emerging evidence shows that drug resistance not only exists against conventional chemotherapeutic drugs, but also limits the efficacy of new biological agents. Therefore, it is important to elucidate the mechanisms through which AML patients develop drug resistance. MicroRNAs have been shown to play an important role in regulating the chemotherapy resistance in AML. A detailed understanding of the mechanisms of microRNA that are clinically relevant in AML may enhance our ability to predict and overcome drug resistance. Here, we demonstrated, for the first time, that miR-181b was decreased significantly in human multidrug-resistant leukemia cells and relapsed/refractory AML patient samples. Overexpression of miR-181b increased the sensitivity of leukemia cells to cytotoxic chemotherapeutic agents and promoted drug-induced apoptosis. Moreover, miR-181b inhibited HMGB1 and Mcl-1 expression by direct binding to their 3'-untranslated regions. In addition, HMGB1 was expressed at high levels in relapsed/refractory AML patients and suppression of HMGB1 via RNA interference sensitized multidrug-resistant leukemia cells to chemotherapy and induced apoptosis. In conclusion, these results provide a strong rationale for the development of miR-181b-based therapeutic strategies for the enhancement of efficacy in AML treatment.

  7. Cultivating adjunct faculty: strategies beyond orientation.

    Science.gov (United States)

    Santisteban, Lisette; Egues, Aida L

    2014-01-01

    Schools of nursing across the country are using adjunct faculty to meet clinical, didactic, and online instructional needs of their nursing programs. While adjunct faculty are vital to the alleviation of the nursing shortage and the shortage of nursing faculty, and to the preparation of the current and future nursing workforce, little is known about cultivating adjunct faculty as nurse educators. To investigate the cultivation of adjunct nursing faculty, the authors engaged in a comprehensive review of the extant literature of primary databases and reports from accredited nursing programs and professional nursing organizations. Scant literature exists that seeks to identify issues associated with developing adjunct nursing faculty as educators, including role transition needs, and useful approaches to orientation, mentorship, or retention. Working toward cultivation of adjunct faculty includes innovative support measures beyond simple orientation. Orientation should be comprehensive, and move to mentorship as a key component that helps establish a sustainable nurse educator career for adjunct nursing faculty. It is incumbent upon schools of nursing to cultivate their adjunct faculty, and this article includes creative approaches to doing so, with recommendations for nursing education, nursing practice, and nursing research settings. While adjunct faculty may successfully meet some of the challenges faced by nursing programs, they themselves face many challenges that may hinder their success as nurse educators. © 2014 Wiley Periodicals, Inc.

  8. The research impact of school psychology faculty.

    Science.gov (United States)

    Watkins, Marley W; Chan-Park, Christina Y

    2015-06-01

    Hirsch's (2005) h index has become one of the most popular indicators of research productivity for higher education faculty. However, the h index varies across academic disciplines so empirically established norms for each discipline are necessary. To that end, the current study collected h index values from Scopus and Google Scholar databases for 401 tenure-track faculty members from 109 school psychology training programs. Male faculty tended to be more senior than female faculty and a greater proportion of the male faculty held professorial rank. However, female faculty members outnumbered males at the assistant and associate professor ranks. Although strongly correlated (rho=.84), h index values from Google Scholar were higher than those from Scopus. h index distributions were positively skewed with many faculty having low values and a few faculty having high values. Faculty in doctoral training programs exhibited significantly larger h index values than faculty in specialist training programs and there were univariate differences in h index values across academic rank and sex, but sex differences were not significant after taking seniority into account. It was recommended that the h index be integrated with peer review and diverse other indicators when considering individual merit. Copyright © 2015 Society for the Study of School Psychology. Published by Elsevier Ltd. All rights reserved.

  9. Production of 34mCl and 38Cl via the (d,α) reaction on 36Ar and natAr gas at 8.4 MeV

    OpenAIRE

    Engle, Jonathan W; Barnhart, Todd E.; DeJesus, Onofre T.; Nickles, Robert J.

    2010-01-01

    Production of 38Cl and 34mCl via the (d, α) reaction on natural argon and 36Ar reveal medical cyclotrons’ ability to supply these isotopes in small quantities. 38Cl provides an inexpensive developmental tool, while 34mCl is a positron emitter of interest in PET imaging. Production yields, cyclotron target development, cryo-recovery of enriched gases, and nucleophilic chemistry of a model compound are described.

  10. Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1

    Directory of Open Access Journals (Sweden)

    Zantl Niko

    2010-06-01

    Full Text Available Abstract Background Human renal cell carcinoma (RCC is very resistant to chemotherapy. ABT-737 is a novel inhibitor of anti-apoptotic proteins of the Bcl-2 family that has shown promise in various preclinical tumour models. Results We here report a strong over-additive pro-apoptotic effect of ABT-737 and etoposide, vinblastine or paclitaxel but not 5-fluorouracil in cell lines from human RCC. ABT-737 showed very little activity as a single agent but killed RCC cells potently when anti-apoptotic Mcl-1 or, unexpectedly, A1 was targeted by RNAi. This potent augmentation required endogenous Noxa protein since RNAi directed against Noxa but not against Bim or Puma reduced apoptosis induction by the combination of ABT-737 and etoposide or vinblastine. At the level of mitochondria, etoposide-treatment had a similar sensitizing activity and allowed for ABT-737-induced release of cytochrome c. Conclusions Chemotherapeutic drugs can overcome protection afforded by Mcl-1 and A1 through endogenous Noxa protein in RCC cells, and the combination of such drugs with ABT-737 may be a promising strategy in RCC. Strikingly, A1 emerged in RCC cell lines as a protein of similar importance as the well-established Mcl-1 in protection against apoptosis in these cells.

  11. The 2004 NASA Faculty Fellowship Program Research Reports

    Science.gov (United States)

    Pruitt, J. R.; Karr, G.; Freeman, L. M.; Hassan, R.; Day, J. B. (Compiler)

    2005-01-01

    This is the administrative report for the 2004 NASA Faculty Fellowship Program (NFFP) held at the George C. Marshall Space Flight Center (MSFC) for the 40th consecutive year. The NFFP offers science and engineering faculty at U.S. colleges and universities hands-on exposure to NASA s research challenges through summer research residencies and extended research opportunities at participating NASA research Centers. During this program, fellows work closely with NASA colleagues on research challenges important to NASA's strategic enterprises that are of mutual interest to the fellow and the Center. The nominal starting and .nishing dates for the 10-week program were June 1 through August 6, 2004. The program was sponsored by NASA Headquarters, Washington, DC, and operated under contract by The University of Alabama, The University of Alabama in Huntsville, and Alabama A&M University. In addition, promotion and applications are managed by the American Society for Engineering Education (ASEE) and assessment is completed by Universities Space Research Association (USRA). The primary objectives of the NFFP are to: Increase the quality and quantity of research collaborations between NASA and the academic community that contribute to the Agency s space aeronautics and space science mission. Engage faculty from colleges, universities, and community colleges in current NASA research and development. Foster a greater public awareness of NASA science and technology, and therefore facilitate academic and workforce literacy in these areas. Strengthen faculty capabilities to enhance the STEM workforce, advance competition, and infuse mission-related research and technology content into classroom teaching. Increase participation of underrepresented and underserved faculty and institutions in NASA science and technology.

  12. Evaluation of doctoral nursing programs in Japan by faculty members and their educational and research activities.

    Science.gov (United States)

    Arimoto, Azusa; Gregg, Misuzu F; Nagata, Satoko; Miki, Yuko; Murashima, Sachiyo

    2012-07-01

    Evaluation of doctoral programs in nursing is becoming more important with the rapid increase in the programs in Japan. This study aimed to evaluate doctoral nursing programs by faculty members and to analyze the relationship of the evaluation with educational and research activities of faculty members in Japan. Target settings were all 46 doctoral nursing programs. Eighty-five faculty members from 28 programs answered the questionnaire, which included 17 items for program evaluation, 12 items for faculty evaluation, 9 items for resource evaluation, 3 items for overall evaluations, and educational and research activities. A majority gave low evaluations for sources of funding, the number of faculty members and support staff, and administrative systems. Faculty members who financially supported a greater number of students gave a higher evaluation for extramural funding support, publication, provision of diverse learning experiences, time of supervision, and research infrastructure. The more time a faculty member spent on advising doctoral students, the higher were their evaluations on the supportive learning environment, administrative systems, time of supervision, and timely feedback on students' research. The findings of this study indicate a need for improvement in research infrastructure, funding sources, and human resources to achieve quality nursing doctoral education in Japan.

  13. Imbalance in liver homeostasis leading to hyperplasia by overexpressing either one of the Bcl-2-related genes, zfBLP1 and zfMcl-1a.

    Science.gov (United States)

    Her, Guor Mour; Cheng, Chih-Hung; Hong, Jiann-Ruey; Sundaram, Gnanapackiam Sheela; Wu, Jen-Leih

    2006-02-01

    Apoptosis is an essential part of normal embryonic development in vertebrates, and it is involved in sculpturing organs and controlling cell populations. In previous studies, we identified two novel proteins, zfBLP1 and zfMcl-1a, which are similar to those of the Bcl-2 family as a group of evolutionarily conserved proteins that regulate cellular anti-apoptosis. To evaluate the effect of dysregulated hepatocyte apoptosis during zebrafish hepatogenesis, we demonstrate the transgenic overexpression of either zfBLP1 or zfMcl-1a in zebrafish larval liver. Results showed that 18%-43% of larvae overexpressed zfBLP1 and that 16%-37% of larvae overexpressed zfMc1-1a in the liver leading to liver hyperplasia in 5-day postfertilization (dpf) zebrafish larvae. Histologically, zebrafish larvae exhibiting liver hyperplasia displayed a normal type of hepatocyte and the same cell numbers in their two liver buds compared with only one liver bud of wild-type larvae. Of interest, the expression of cyclin genes (A2, B, D1, and E), hepatocyte nuclear factor genes (HNF-1alpha, beta, -3beta, and 4alpha), and oncogenic markers (P53, c-myc, beta-catenin, N-ras, and gankyrin) were up-regulated, while the expression of C/EBP-alpha was down-regulated in a zfMcl-1a-mediated anti-apoptotic process of the liver. Increased cell death and proliferation was found in both hepatic cells of zebrafish larvae overexpressing either zfBLP1 or zfMcl-1a. However, those zebrafish larvae with liver hyperplasia only lived approximately 10 days. (This finding may have been due to liver abnormalities that led to failure of liver function.) In conclusion, transgenic overexpression of zfBLP1 or zfMcl-1a in zebrafish larvae interrupts regulation of the homeostatic balance between cell proliferation and programmed cell death during hepatogenesis and leads to liver hyperplasia. Copyright 2005 Wiley-Liss, Inc.

  14. Trichonomas vaginalis metalloproteinase induces apoptosis of SiHa cells through disrupting the Mcl-1/Bim and Bcl-xL/Bim complexes.

    Science.gov (United States)

    Quan, Juan-Hua; Kang, Byung-Hun; Cha, Guang-Ho; Zhou, Wei; Koh, Young-Bok; Yang, Jung-Bo; Yoo, Heon-Jong; Lee, Min-A; Ryu, Jae-Sook; Noh, Heung-Tae; Kwon, Jaeyul; Lee, Young-Ha

    2014-01-01

    To elucidate the roles of metalloproteinases and the Bcl-2 family of proteins in Trichovaginalis. vaginalis-induced apoptosis in human cervical cancer cells (SiHa cells) and vaginal epithelial cells (MS74 cells), SiHa cells and MS74 cells were incubated with live T. vaginalis, T. vaginalis excretory and secretory products (ESP), and T. vaginalis lysates, either with or without the specific metalloproteinase inhibitor 1,10-phenanthroline (1,10-PT), and examined apoptotic events and Bcl-2 signaling. The live T. vaginalis and the T. vaginalis ESP induced the release of cytochrome c into the cytosol, the activation of caspase-3 and caspase-9, and the cleavage of PARP. Additionally, the live T. vaginalis, but not the T. vaginalis lysate, induced the cleavage of the proapoptotic Bim protein. The live T. vaginalis and the T. vaginalis ESP, but not the T. vaginalis lysate, induced the dose-dependent cleavage of the antiapoptotic Bcl-xL and Mcl-1 proteins and decreased the association levels of Bcl-xL/Bim and Mcl-1/Bim complexes. We performed gelatin zymography and casein-hydrolysis assays on the live T. vaginalis and the T. vaginalis ESP to identify the apoptosis-inducing factor. Both the live T. vaginalis and the ESP contained high levels of metalloproteinases, of which activities were significantly inhibited by 1,10-PT treatment. Furthermore, the 1,10-PT blocked the cleavage of Bcl-xL, Mcl-1, PARP, caspase-3, and caspase-9, as well as the release of cytochrome c into the cytosol, and it significantly increased the association levels of the Bcl-xL/Bim and Mcl-1/Bim protein complexes, returning them to normal levels. Our results demonstrate that T. vaginalis induces mitochondria-dependent apoptosis in SiHa cells through the dissociation of Bcl-xL/Bim and Mcl-1/Bim complexes and that the apoptosis is blocked by the metalloproteinase inhibitor 1,10-PT. These results expand our understanding of the role of metalloproteinases in T. vaginalis-induced apoptosis and the signaling

  15. Trichonomas vaginalis metalloproteinase induces apoptosis of SiHa cells through disrupting the Mcl-1/Bim and Bcl-xL/Bim complexes.

    Directory of Open Access Journals (Sweden)

    Juan-Hua Quan

    Full Text Available To elucidate the roles of metalloproteinases and the Bcl-2 family of proteins in Trichovaginalis. vaginalis-induced apoptosis in human cervical cancer cells (SiHa cells and vaginal epithelial cells (MS74 cells, SiHa cells and MS74 cells were incubated with live T. vaginalis, T. vaginalis excretory and secretory products (ESP, and T. vaginalis lysates, either with or without the specific metalloproteinase inhibitor 1,10-phenanthroline (1,10-PT, and examined apoptotic events and Bcl-2 signaling. The live T. vaginalis and the T. vaginalis ESP induced the release of cytochrome c into the cytosol, the activation of caspase-3 and caspase-9, and the cleavage of PARP. Additionally, the live T. vaginalis, but not the T. vaginalis lysate, induced the cleavage of the proapoptotic Bim protein. The live T. vaginalis and the T. vaginalis ESP, but not the T. vaginalis lysate, induced the dose-dependent cleavage of the antiapoptotic Bcl-xL and Mcl-1 proteins and decreased the association levels of Bcl-xL/Bim and Mcl-1/Bim complexes. We performed gelatin zymography and casein-hydrolysis assays on the live T. vaginalis and the T. vaginalis ESP to identify the apoptosis-inducing factor. Both the live T. vaginalis and the ESP contained high levels of metalloproteinases, of which activities were significantly inhibited by 1,10-PT treatment. Furthermore, the 1,10-PT blocked the cleavage of Bcl-xL, Mcl-1, PARP, caspase-3, and caspase-9, as well as the release of cytochrome c into the cytosol, and it significantly increased the association levels of the Bcl-xL/Bim and Mcl-1/Bim protein complexes, returning them to normal levels. Our results demonstrate that T. vaginalis induces mitochondria-dependent apoptosis in SiHa cells through the dissociation of Bcl-xL/Bim and Mcl-1/Bim complexes and that the apoptosis is blocked by the metalloproteinase inhibitor 1,10-PT. These results expand our understanding of the role of metalloproteinases in T. vaginalis-induced apoptosis

  16. faculties

    Directory of Open Access Journals (Sweden)

    Mardanov Rustam Sh.

    2016-01-01

    Full Text Available The paper analyzes some of the psychological problems of the economic faculties’ students arising in the course of study of mathematical disciplines. These problems are primarily related with the lack of students’ awareness, misconception about the calculation methods in their future profession, low motivation and performance, math anxiety, etc. They makeadditional difficulties which impede successful mastering of sophisticated material. Based on the experience of mathematical disciplines teaching, the paper provides some possible solutions to these problems with the lecturer who has to play an important role. The lecturer should orientate students towards serious and profound knowledge of economic and mathematical methods, create conditions for students’ active participation in the educational process and provide them with comprehensive assistance in overcoming difficulties.

  17. Faculty development in medical education research.

    Science.gov (United States)

    LaMantia, Joseph; Hamstra, Stanley J; Martin, Daniel R; Searle, Nancy; Love, Jeffrey; Castaneda, Jill; Aziz-Bose, Rahela; Smith, Michael; Griswold-Therodorson, Sharon; Leuck, JoAnna

    2012-12-01

    This 2012 Academic Emergency Medicine consensus conference breakout session was devoted to the task of identifying the history and current state of faculty development in education research in emergency medicine (EM). The participants set a future agenda for successful faculty development in education research. A number of education research and content experts collaborated during the session. This article summarizes existing academic and medical literature, expert opinions, and audience consensus to report our agreement and findings related to the promotion of faculty development.

  18. Performance Measurement and Faculty Pay

    Institute of Scientific and Technical Information of China (English)

    LUO Pin-liang; RUI Ming-jie

    2002-01-01

    In classic agency models, first best efficiency can't be achieved due to the trade-off between risk and incentives except that agency is risk neutral. Whereas if the principal's objective is not contractible,an alternative objective performance measurement which is contractible is always proposed. However, if the reaction of this objective performance measurement to agency's effort differs from that of the principal's objective, the agent would game performance measures, which leads to loss of efficiency, even if agency is risk neutral. By adding subjective weights on objective measures, or combination of subjective performance measurement with objective performance measurement, efficiency can be regained. Implications for faculty pay are also discussed.

  19. Faculty role modeling of professional writing: one baccalaureate nursing program's experience.

    Science.gov (United States)

    Newton, Sarah E

    2008-01-01

    According to The Essentials of Baccalaureate Education for Professional Nursing Practice (American Association of Colleges of Nursing, 1998), professional writing is an important outcome of baccalaureate nursing education. Most baccalaureate nursing programs in the United States expect formally written student papers to adhere to the style requirements outlined in the Publication Manual of the American Psychological Association (APA, 2001). It is essential for the baccalaureate nursing faculty members who evaluate student papers to be role models for the desired writing behaviors to facilitate student attainment of professional writing outcomes. However, to what extent nursing faculty members' writing behaviors and knowledge of the APA style requirements impact student writing outcomes is not known because the issue has not been addressed in the literature. The purpose of this article is to describe one Midwestern baccalaureate nursing program's faculty development efforts to assess faculty familiarity with the APA style requirements and how such knowledge may impact baccalaureate nursing students' writing outcomes.

  20. Motivational Factors Affecting the Integration of a Learning Management System by Faculty

    Directory of Open Access Journals (Sweden)

    Cynthia Gautreau

    2011-01-01

    Full Text Available Online courses taught using a learning management system are common in higher education. Teaching online requires a new set of skills, knowledge, and professional growth. Faculty development programs often overlook factors that promote or inhibit the use of technologies among professors. This study identified the motivation factors that faculty consider relevant to their personal decision to adopt a learning management system. A needs assessment evaluation methodology was applied to investigate two research questions. The first question analyzed the demographics of the participants in this study including gender, age, tenure status, department, and years of experience using a technology and using an LMS. The second research question investigated the intrinsic and extrinsic factors that motivate faculty to adopt a learning management system in their instruction. Participants (N = 42 were tenured and tenure track faculty instructing at a four-year public university in California.

  1. Hardin-Simmons University Faculty Handbook, 1975-1976.

    Science.gov (United States)

    Hardin-Simmons Univ., Abilene, TX.

    The 1975 edition of the faculty handbook is divided into major sections covering administrative structure, faculty-administration relationships, faculty compensation and fringe benefits, faculty services, faculty-student responsibilities and relationships, and summer school employment. The university administration is described with regard to the…

  2. Faculty Rank System, Research Motivation, and Faculty Research Productivity: Measure Refinement and Theory Testing.

    Science.gov (United States)

    Tien, Flora F.; Blackburn, Robert T.

    1996-01-01

    A study explored the relationship between the traditional system of college faculty rank and faculty research productivity from the perspectives of behavioral reinforcement theory and selection function. Six hypotheses were generated and tested, using data from a 1989 national faculty survey. Results failed to support completely either the…

  3. Expanding the Discussion of Faculty Vitality to Include Productive but Disengaged Senior Faculty

    Science.gov (United States)

    Huston, Therese A.; Norman, Marie; Ambrose, Susan A.

    2007-01-01

    In this essay, the authors begin by examining and challenging the way in which faculty vitality has been operationalized in the past, arguing for the value of institution-specific analysis of the faculty vitality issue. They then propose alternative models for understanding previously unexplored aspects of faculty vitality, drawing on research in…

  4. Net Income of Pharmacy Faculty Compared to Community and Hospital Pharmacists.

    Science.gov (United States)

    Chisholm-Burns, Marie A; Gatwood, Justin; Spivey, Christina A; Dickey, Susan E

    2016-09-25

    Objective. To compare the net cumulative income of community pharmacists, hospital pharmacists, and full-time pharmacy faculty members (residency-trained or with a PhD after obtaining a PharmD) in pharmacy practice, medicinal chemistry, pharmaceutics, pharmacology, and social and administrative sciences. Methods. Markov modeling was conducted to calculate net projected cumulative earnings of career paths by estimating the costs of education, including the costs of obtaining degrees and student loans. Results. The economic model spanned 49 years, from ages 18 to 67 years. Earning a PharmD and pursuing an academic career resulted in projected net cumulative lifetime earnings ranging from approximately $4.7 million to $6.3 million. A pharmacy practice faculty position following public pharmacy school and one year of residency resulted in higher net cumulative income than community pharmacy. Faculty members with postgraduate year 1 (PGY1) training also had higher net income than other faculty and hospital pharmacy career paths, given similar years of prepharmacy education and type of pharmacy school attended. Faculty members with either a PharmD or PhD in the pharmacology discipline may net as much as $5.9 million and outpace all other PhD graduates by at least $75 000 in lifetime earnings. Projected career earnings of postgraduate year 2 (PGY2) trained faculty and PharmD/PhD faculty members were lower than those of community pharmacists. Findings were more variable when comparing pharmacy faculty members and hospital pharmacists. Conclusion. With the exception of PGY1 trained academic pharmacists, faculty projected net cumulative incomes generally lagged behind community pharmacists, likely because of delayed entry into the job market as a result of advanced training/education. However, nonsalary benefits such as greater flexibility and autonomy may enhance the desirability of academic pharmacy as a career path.

  5. Net Income of Pharmacy Faculty Compared to Community and Hospital Pharmacists

    Science.gov (United States)

    Gatwood, Justin; Spivey, Christina A.; Dickey, Susan E.

    2016-01-01

    Objective. To compare the net cumulative income of community pharmacists, hospital pharmacists, and full-time pharmacy faculty members (residency-trained or with a PhD after obtaining a PharmD) in pharmacy practice, medicinal chemistry, pharmaceutics, pharmacology, and social and administrative sciences. Methods. Markov modeling was conducted to calculate net projected cumulative earnings of career paths by estimating the costs of education, including the costs of obtaining degrees and student loans. Results. The economic model spanned 49 years, from ages 18 to 67 years. Earning a PharmD and pursuing an academic career resulted in projected net cumulative lifetime earnings ranging from approximately $4.7 million to $6.3 million. A pharmacy practice faculty position following public pharmacy school and one year of residency resulted in higher net cumulative income than community pharmacy. Faculty members with postgraduate year 1 (PGY1) training also had higher net income than other faculty and hospital pharmacy career paths, given similar years of prepharmacy education and type of pharmacy school attended. Faculty members with either a PharmD or PhD in the pharmacology discipline may net as much as $5.9 million and outpace all other PhD graduates by at least $75 000 in lifetime earnings. Projected career earnings of postgraduate year 2 (PGY2) trained faculty and PharmD/PhD faculty members were lower than those of community pharmacists. Findings were more variable when comparing pharmacy faculty members and hospital pharmacists. Conclusion. With the exception of PGY1 trained academic pharmacists, faculty projected net cumulative incomes generally lagged behind community pharmacists, likely because of delayed entry into the job market as a result of advanced training/education. However, nonsalary benefits such as greater flexibility and autonomy may enhance the desirability of academic pharmacy as a career path. PMID:27756925

  6. Faculty Internationalization: Experiences, Attitudes, and Involvement of Faculty at Public Universities in South Dakota

    Science.gov (United States)

    Doyle, Kevin

    2013-01-01

    Internationalization in higher education is an issue of growing importance as the forces of globalism continue to push both social and economic connections from local to global. While this topic is becoming increasingly vital to the health and influence of educational institutions, many, including those within South Dakota, are unaware of the…

  7. Faculty Internationalization: Experiences, Attitudes, and Involvement of Faculty at Public Universities in South Dakota

    Science.gov (United States)

    Doyle, Kevin

    2013-01-01

    Internationalization in higher education is an issue of growing importance as the forces of globalism continue to push both social and economic connections from local to global. While this topic is becoming increasingly vital to the health and influence of educational institutions, many, including those within South Dakota, are unaware of the…

  8. Minimal residual disease monitoring by 8-color flow cytometry in mantle cell lymphoma: an EU-MCL and LYSA study

    Science.gov (United States)

    Cheminant, Morgane; Derrieux, Coralie; Touzart, Aurore; Schmit, Stéphanie; Grenier, Adrien; Trinquand, Amélie; Delfau-Larue, Marie-Hélène; Lhermitte, Ludovic; Thieblemont, Catherine; Ribrag, Vincent; Cheze, Stéphane; Sanhes, Laurence; Jardin, Fabrice; Lefrère, François; Delarue, Richard; Hoster, Eva; Dreyling, Martin; Asnafi, Vahid; Hermine, Olivier; Macintyre, Elizabeth

    2016-01-01

    Quantification of minimal residual disease may guide therapeutic strategies in mantle cell lymphoma. While multiparameter flow cytometry is used for diagnosis, the gold standard method for minimal residual disease analysis is real-time quantitative polymerase chain reaction (RQ-PCR). In this European Mantle Cell Lymphoma network (EU-MCL) pilot study, we compared flow cytometry with RQ-PCR for minimal residual disease detection. Of 113 patients with at least one minimal residual disease sample, RQ-PCR was applicable in 97 (86%). A total of 284 minimal residual disease samples from 61 patients were analyzed in parallel by flow cytometry and RQ-PCR. A single, 8-color, 10-antibody flow cytometry tube allowed specific minimal residual disease assessment in all patients, with a robust sensitivity of 0.01%. Using this cut-off level, the true-positive-rate of flow cytometry with respect to RQ-PCR was 80%, whereas the true-negative-rate was 92%. As expected, RQ-PCR frequently detected positivity below this 0.01% threshold, which is insufficiently sensitive for prognostic evaluation and would ideally be replaced with robust quantification down to a 0.001% (10-5) threshold. In 10 relapsing patients, the transition from negative to positive by RQ-PCR (median 22.5 months before relapse) nearly always preceded transition by flow cytometry (4.5 months), but transition to RQ-PCR positivity above 0.01% (5 months) was simultaneous. Pre-emptive rituximab treatment of 2 patients at minimal residual disease relapse allowed re-establishment of molecular and phenotypic complete remission. Flow cytometry minimal residual disease is a complementary approach to RQ-PCR and a promising tool in individual mantle cell lymphoma therapeutic management. PMID:26703963

  9. Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl-PHAs) by Comamonas testosteroni during cultivation on vegetable oils.

    Science.gov (United States)

    Thakor, Nehal; Trivedi, Ujjval; Patel, K C

    2005-11-01

    Comamonas testosteroni has been studied for its ability to synthesize and accumulate medium chain length poly(3-hydroxyalkanoates) (mcl-PHAs) during cultivation on vegetable oils available in the local market. Castor seed oil, coconut oil, mustard oil, cotton seed oil, groundnut oil, olive oil and sesame oil were supplemented in the mineral medium as a sole source of carbon for growth and PHAs accumulation. The composition of PHAs was analysed by a coupled gas chromatography/mass spectroscopy (GC/MS). PHAs contained C6 to C14 3-hydroxy acids, with a strong presence of 3-hydroxyoctanoate when coconut oil, mustard oil, cotton seed oil and groundnut oil were supplied. 3-hydroxydecanoate was incorporated at higher concentrations when castor seed oil, olive oil and sesame oil were the substrates. Purified PHAs samples were characterized by Fourier Transform Infrared (FTIR) and 13C NMR analysis. During cultivation on various vegetable oils, C. testosteroni accumulated PHAs up to 78.5-87.5% of the cellular dry material (CDM). The efficiency of the culture to convert oil to PHAs ranged from 53.1% to 58.3% for different vegetable oils. Further more, the composition of the PHAs formed was not found to be substrate dependent as PHAs obtained from C. testosteroni during growth on variety of vegetable oils showed similar compositions; 3-hydroxyoctanoic acid and/or 3-hydroxydecanoic acid being always predominant. The polymerizing system of C. testosteroni showed higher preference for C8 and C10 monomers as longer and smaller monomers were incorporated less efficiently.

  10. miR-26a-5p调控MCL-1在孕产妇子痫前期中表达的临床意义%Clinical significance of miR-26a-5p-regulated MCL-1 expression in pre-eclampsia

    Institute of Scientific and Technical Information of China (English)

    杨莹; 苟文丽; 谷寅; 程黎; 赖彩琴; 鞠叶兰; 王晨虹

    2015-01-01

    目的:探讨microRNA-26a-5p(miR-26a-5p)调控髓细胞白血病因子1(myeloid cell leukemia -1, MCL-1)表达在孕产妇子痫前期发生发展中的临床意义。方法:收集正常妊娠21例、妊娠期高血压孕产妇13例、轻度子痫前期15例和重度子痫前期26例共4组孕产妇血浆及胎盘组织,用real-time PCR分析其血浆及胎盘组织中miR-26a-5p及胎盘组织中MCL-1 mRNA的表达,Western blotting分析胎盘组织中MCL-1蛋白的表达,并分析其表达的临床意义。结果:随着病情进展,miR-26a-5p在孕产妇血浆和胎盘中表达逐步增高(P<0.01),而其胎盘组织中MCL-1 mRNA的表达明显降低(P<0.01),二者呈明显负相关(P<0.01);胎盘组织中miR-26a-5p与MCL-1蛋白表达呈明显负相关( P<0.01)。孕产妇血浆及胎盘组织中miR-26a-5p表达上调与孕龄、孕妇血浆白蛋白水平及胎儿体重呈明显负相关,而与孕产妇血压和尿蛋白水平呈明显正相关(P<0.01),胎盘组织中MCL-1表达下调与此相反。结论:miR-26a-5p高表达通过下调MCL-1的表达参与子痫前期的发生与发展。%AIM:To investigate the clinical significance of microRNA-26a-5p (miR-26a-5p)-regulated mye-loid cell leukemia-1 (MCL-1) expression in the development of maternal preeclampsia.METHODS:Plasma and placen-tal tissues were collected from 21 cases of normal pregnancy, 13 cases of maternal gestational hypertension, 15 cases of mild preeclampsia and 26 cases of severe preeclampsia.The levels of plasma and placental miR-26a-5p and placental MCL-1 mRNA were detected by real-time PCR.Western blotting analysis was used to determine the protein expression of placen-tal MCL-1.The clinical significance of the above parameters was also analyzed.RESULTS:miR-26a-5p expression gradu-ally increased(P<0.01) in the 4 groups of maternal plasma and placentas with the disease development, and the mRNA expression of MCL-1 was significantly

  11. Proyección del estudiante de Patología y Clínica Estomatológica desde el ámbito del claustro de la Facultad al Hospital Público: Registro retrospectivo de las patologías observadas Projection of oral pathology and Clinical Stomatology student from faculty class room to public hospital: Retrospective analyse of the observed diseases

    Directory of Open Access Journals (Sweden)

    ML Mercado

    2006-10-01

    realidad de la salud pública del medio ambiente al cual pertenece. 2 Las demás especialidades, integradas al Servicio de Odontología/Estomatología, permitirían una acción en conjunto del equipo sanitario, por un fenómeno de retroalimentación. 3 Coincidiendo con otros datos de diversos Servicios Asistenciales y otras Facultades del país, la Candidiasis oral resultó la lesión más frecuentemente observada. 4 La incidencia de Cáncer bucal nos lleva a insistir sobre su diagnóstico temprano y la importancia del reconocimiento de las enfermedades precancerosa y establecer estrategias contra el hábito del tabaquismo, en nuestro medio de referencia.This study was designed in order to establish basement of Teaching/Learning process in Stomatology, for students of the last step of Curricula in Odontology and, very specially for Patology and Stomatological Clinic’ attenden. Objectives were: 1 Observation of students behaviour in a public hospital, were it takes place, their «work in situ». 2 To stand out diagnosticated lesions and diseases with the guide of experimental teachers and, resolution of clinic cases. Material and methods: Students of the last level of Patology and Clinical Stomatology Asignature, of the Odontology Faculty of La Plata City, year by year were sending in small groups (no more than seven students each, to a Public Hospital, to Odontology Unit, to participate in an equipment composed by Docents of the Faculty that works in this hospitalry Service and in relationship with other medical units of the same Hospital. For this study, data was assesed taking 1.800 hours/patient. 495 patients were evaluated and sex, age, civil state, ocupation, habits and factors of the enviroment were tabulated. Screaning of lesions and diseases were made and an statistic analyse was performed. Results: 1 Students demostrated, most of the time, really interesting and entusiassm. This result was calculated in a frequence of 40% of them, who wanted to state longer

  12. Factors Associated with Veterinary Clinical Faculty Attrition.

    Science.gov (United States)

    Furr, Martin

    2017-06-28

    Faculty attrition and recruitment for veterinary clinical faculty positions have been reported as significant problems in veterinary medical education. To investigate the factors that may be important in veterinary clinical faculty retention, the perceptions and views of veterinary clinical academic faculty were determined using a web-distributed electronic survey. Responses were dichotomized by whether the respondent had or had not left an academic position and were analyzed for their association with faculty attrition. A total of 1,226 responses were recorded and results demonstrated that factors other than compensation were associated with veterinary clinical faculty attrition, including departmental culture, work-life balance, and recognition and support of clinical medicine by the administration. Forty-four percent of respondents who had held a faculty appointment reported leaving academia either voluntarily or for non-voluntary reasons such as failure to achieve tenure, retirement, or having their position closed. Attention to correcting deficiencies in workplace culture and professional rewards could be a beneficial means by which to decrease the faculty attrition rates currently observed in clinical academic veterinary medicine.

  13. Teaching Evaluations: Perceptions of Students and Faculty

    Science.gov (United States)

    Mukherji, Sandip; Rustagi, Narendra

    2008-01-01

    This study conducts a survey of students and faculty at a business school on critical issues regarding student evaluations of teaching and identifies several significant differences between their perceptions. Students agreed more strongly than faculty that evaluations are higher in courses where the instructor teaches effectively and students…

  14. Motivational Issues of Faculty in Saudi Arabia

    Science.gov (United States)

    Abdul Cader, Akram

    2012-01-01

    Researchers have suggested that faculty motivation influences profitability of academic programs. The problem researched in this mixed method study was the motivational factors that reduce faculty member effectiveness in improving the profitability of their universities' academic programs. Based on Maslow's theory of needs, the purpose of the…

  15. Aging in the Undergraduate Curriculum: Faculty Perspectives

    Science.gov (United States)

    Heyman, Janna C.; Gutheil, Irene A.; White-Ryan, Linda; Phipps, Colette; Guishard, Dozene

    2008-01-01

    This descriptive study of undergraduate faculty (N = 177) ascertained the extent to which aging content is taught and faculty are interested in aging. The research was the result of a collaboration among an area agency on aging, an alliance of academic and community leaders, and a university-based research center. While approximately 43% of the…

  16. Enhancing Sustainability Curricula through Faculty Learning Communities

    Science.gov (United States)

    Natkin, L. W.; Kolbe, Tammy

    2016-01-01

    Purpose: Although the number of higher education institutions adopting sustainability-focused faculty learning communities (FLCs) has grown, very few of these programs have published evaluation research. This paper aims to report findings from an evaluation of the University of Vermont's (UVM's) sustainability faculty fellows (SFF) program. It…

  17. The organisational aspect of faculty development

    DEFF Research Database (Denmark)

    Kolmos, Anette; Gynnild, Vidar; Roxå, Torgny

    2004-01-01

    The article points out the faculty centres ought to be more conscious in their organisational strategies and get to managements support when working on pedagogical changes.......The article points out the faculty centres ought to be more conscious in their organisational strategies and get to managements support when working on pedagogical changes....

  18. A Service-Learning Curriculum for Faculty.

    Science.gov (United States)

    Bringle, Robert G.; Hatcher, Julie A.

    1995-01-01

    Suggests that higher education institutions should provide faculty development opportunities for service-learning that develop a common understanding on campus concerning the nature of service-learning, establish and maintain academic integrity of service-learning; increase faculty confidence in implementing a new pedagogy, and increase the…

  19. Harvard Law School's War over Faculty Diversity.

    Science.gov (United States)

    Bromberg, Matthew S.

    1993-01-01

    Examines the controversy over the lack of faculty diversity at Harvard Law School and highlights the school's past practices regarding hiring and promotion of minority teaching staff. The pool problem issue is discussed, and the current status of faculty diversity is presented. (GLR)

  20. Faith and Faculty Autonomy at Calvin College.

    Science.gov (United States)

    Monsma, George N., Jr.

    2001-01-01

    Asserts that to prevent incursions on academic freedom, faculty members must make a strong commitment of time and expertise to institutional governance, and they must promote regulations and behavior that support academic freedom. Describes how the administration, faculty, and board of Calvin College, affiliated with the Christian Reformed Church,…

  1. Nursing Faculty: One Generation away from Extinction?

    Science.gov (United States)

    Brendtro, Mary; Hegge, Marge

    2000-01-01

    A statewide survey of 288 nurses with graduate degrees found that those were nursing faculty (n=79) were older than other nurses in the sample. There were no differences in job satisfaction between faculty and other nurses. Noncompetitive salaries, desire for clinical practice, and rising expectations in higher education were deterrents to…

  2. Factors Explaining Faculty Technology Use and Productivity

    Science.gov (United States)

    Xu, Yonghong; Meyer, Katrina A.

    2007-01-01

    This study examines factors related to technology use in teaching by university faculty. An EFA analysis of multiple questions of technology use in the classroom found two factors: one loaded with Web use and the second with email use. Therefore, three research questions were asked: What factors explain faculty use of the Web or email? Are these…

  3. Bullying and Inappropriate Behaviour among Faculty Personnel

    Science.gov (United States)

    Meriläinen, Matti; Sinkkonen, Hanna-Maija; Puhakka, Helena; Käyhkö, Katinka

    2016-01-01

    This study focuses on the degree, nature and consequences of bullying or inappropriate behaviour among faculty personnel (n = 303) in a Finnish university. A total of 114 (38%) faculty members answered the email questionnaire. According to the results, 15% of the respondents had experienced bullying; in addition, 45% had experienced inappropriate…

  4. The Madness of Weighted Mean Faculty Salaries

    Science.gov (United States)

    Micceri, Theodore

    2009-01-01

    Higher education frequently uses weighted mean faculty salaries to compare either across institutions, or to evaluate an institution's salary growth over time. Unfortunately, faculty salaries are an extraordinarily complex phenomenon that cannot be legitimately reduced to a single number any more than the academic construct of skills, knowledge,…

  5. Faculty Development: An Imperative for the Nineties.

    Science.gov (United States)

    Nies, Joyce I.

    1990-01-01

    Budget constraints and changing enrollment patterns have expanded the concept of faculty development to include retraining. In home economics, retraining faculty for high demand areas such as hotel/restaurant management and fashion merchandising can be an efficient use of resources and an effective way to meet demand. (SK)

  6. Rising Tides: Faculty Expectations of Library Websites

    Science.gov (United States)

    Nicol, Erica Carlson; O'English, Mark

    2012-01-01

    Looking at 2003-2009 LibQUAL+ responses at research-oriented universities in the United States, faculty library users report a significant and consistent rise in desires and expectations for library-provided online tools and websites, even as student user groups show declining or leveling expectations. While faculty, like students, also report…

  7. Social Work Faculty and Undergraduate Research Mentorships

    Science.gov (United States)

    Horner, Pilar S.; Hughes, Anne K.; Vélez Ortiz, Daniel

    2016-01-01

    Social work faculty scholars lead the field as generators of knowledge that integrates investigative studies with practical social welfare outcomes. As such, the faculty potentially offers undergraduate researchers a different way of envisioning research that extends beyond traditional undergraduate research models. To date, however, no research…

  8. How to Evaluate a Faculty Governance Structure

    Science.gov (United States)

    Cordes, John W.; Dunbar, David; Gingerich, Jeff

    2013-01-01

    During the 2010-11 academic year, Cabrini College began an evaluation of a faculty governance structure that had been implemented in fall 2007. The processes involved might serve as a roadmap for faculty members and administrators at other institutions who seek to understand the strengths and weaknesses of their governance model and improve shared…

  9. Business Students' Ethical Evaluations of Faculty Misconduct

    Science.gov (United States)

    Valentine, Sean; Kidwell, Roland E.

    2008-01-01

    Purpose: This study aims to gauge business school student perceptions of the academic conduct of college professors, to determine students' ethical evaluations of certain potential faculty behaviors. The relationships between perceived faculty misconduct and several student demographic characteristics including sex and academic classification were…

  10. Study of Faculty and Information Technology, 2014

    Science.gov (United States)

    Dahlstrom, Eden; Brooks, D. Christopher

    2014-01-01

    In this inaugural year of the faculty technology study, EDUCAUSE Center for Analysis and Research (ECAR) partnered with 151 college/university sites yielding responses from 17,451 faculty respondents across 13 countries. The findings are exploratory in nature, as they cover new ground to help us tell a more comprehensive story about technology…

  11. AACSB Standards and Accounting Faculty's Intellectual Contributions

    Science.gov (United States)

    Lee, B. Brian; Quddus, Munir

    2008-01-01

    The authors performed a content analysis of intellectual contribution portfolios of accounting faculty at various business schools that Association to Advance Collegiate Schools of Business International recently accredited. The results showed a significant divergence in faculty research (e.g., areas, topics) and their teaching assignments. This…

  12. Confidentiality and Faculty Representation in Academic Governance

    Science.gov (United States)

    American Association of University Professors, 2013

    2013-01-01

    This report argues that requiring faculty members to sign confidentiality agreements as a requirement to serve on university committees is in most cases inconsistent with widely accepted standards of shared governance and with the concept of serving as a representative. This argument does not apply to faculty serving on promotion and tenure…

  13. Ethical Perspectives on Evaluating Community College Faculty

    Science.gov (United States)

    Stumpf, Dan; King, Stephanie; Blendinger, Jack; Davis, Ed

    2013-01-01

    Because the process of faculty evaluation in the community college gives rise to ethical concerns about what is evaluated, who is involved in the process, and how data are collected and used, the purpose of this paper is to provide a meaningful ethical perspective for conducting faculty evaluation. The authors discuss ethical issues that arise in…

  14. Engaging Faculty across the Community Engagement Continuum

    Science.gov (United States)

    Gorski, Irena; Mehta, Khanjan

    2016-01-01

    There currently exists an incompatibility between the demands of university administrators for increased community engagement and the realities facing faculty who want to integrate it into their academic coursework, research, and professional service. This article provides insight on the complex challenges preventing faculty from becoming involved…

  15. Predicting Seminary Faculty Engagement with Multicultural Education

    Science.gov (United States)

    Gin, Deborah Hearn-Chung

    2012-01-01

    Most multicultural theological education research has focused on theoretical or historical pieces and only on a few institutions. This study explored the personal, professional, institutional, and interactional predictors of seminary faculty engagement with multicultural education. Three hundred full-time faculty in U.S. seminaries affiliated with…

  16. Student and Faculty Ethnic Diversity Report

    Science.gov (United States)

    Greiner, Keith; Girardi, Anthony G.

    2007-01-01

    The annual Ethnic Diversity Report provides information about minority student enrollment and minority faculty at Iowa colleges and universities. The "Student and Faculty Ethnic Diversity Report" has been prepared annually since 1992 and is provided to the Governor and General Assembly leadership. This summary is based on a Fall 2006…

  17. Senior Faculty Perceptions of Social Work Journals.

    Science.gov (United States)

    Cnaan, Ram A.; And Others

    1994-01-01

    A survey of 421 senior faculty in graduate social work education investigated the familiarity and perceived quality of 120 professional journals in the field. Resulting ratings are presented for use by faculty seeking to publish their work in appropriate journals and those assessing the scholarly contribution of social work educators. (Author/MSE)

  18. Faculty on Facebook: Confirm or Deny?

    Science.gov (United States)

    Sturgeon, C. Michael; Walker, Christin

    2009-01-01

    Since its creation in 2004, Facebook has become one of the most frequently visited websites on college campuses. Because of this rise in popularity, the subject of social networking has grown as an idea and concern for both faculty members and students. At Lee University, it has been observed that a growing number of faculty members have indeed…

  19. Enhancing Sustainability Curricula through Faculty Learning Communities

    Science.gov (United States)

    Natkin, L. W.; Kolbe, Tammy

    2016-01-01

    Purpose: Although the number of higher education institutions adopting sustainability-focused faculty learning communities (FLCs) has grown, very few of these programs have published evaluation research. This paper aims to report findings from an evaluation of the University of Vermont's (UVM's) sustainability faculty fellows (SFF) program. It…

  20. Aging in the Undergraduate Curriculum: Faculty Perspectives

    Science.gov (United States)

    Heyman, Janna C.; Gutheil, Irene A.; White-Ryan, Linda; Phipps, Colette; Guishard, Dozene

    2008-01-01

    This descriptive study of undergraduate faculty (N = 177) ascertained the extent to which aging content is taught and faculty are interested in aging. The research was the result of a collaboration among an area agency on aging, an alliance of academic and community leaders, and a university-based research center. While approximately 43% of the…

  1. Faculty Development: An Imperative for the Nineties.

    Science.gov (United States)

    Nies, Joyce I.

    1990-01-01

    Budget constraints and changing enrollment patterns have expanded the concept of faculty development to include retraining. In home economics, retraining faculty for high demand areas such as hotel/restaurant management and fashion merchandising can be an efficient use of resources and an effective way to meet demand. (SK)

  2. Student versus Faculty Perceptions of Missing Class.

    Science.gov (United States)

    Sleigh, Merry J.; Ritzer, Darren R.; Casey, Michael B.

    2002-01-01

    Examines and compares student and faculty attitudes towards students missing classes and class attendance. Surveys undergraduate students (n=231) in lower and upper level psychology courses and psychology faculty. Reports that students found more reasons acceptable for missing classes and that the amount of in-class material on the examinations…

  3. Predicting Faculty Integration of Faith and Learning

    Science.gov (United States)

    Kaul, Corina R.; Hardin, Kimberly A.; Beaujean, A. Alexander

    2017-01-01

    Concern regarding the secularization of Christian higher education has prompted researchers to investigate the extent that faith and learning is integrated at a faculty level and what factors might predict faculty integration (Lyon, Beaty, Parker, & Mencken, 2005). This research attempted to replicate Lyon et al.'s (2005) logistic regression…

  4. AACSB Standards and Accounting Faculty's Intellectual Contributions

    Science.gov (United States)

    Lee, B. Brian; Quddus, Munir

    2008-01-01

    The authors performed a content analysis of intellectual contribution portfolios of accounting faculty at various business schools that Association to Advance Collegiate Schools of Business International recently accredited. The results showed a significant divergence in faculty research (e.g., areas, topics) and their teaching assignments. This…

  5. Nursing Faculty Perceptions on Teaching Critical Thinking

    Science.gov (United States)

    Clark, Doris A.

    2010-01-01

    The perceptions of nursing faculty teaching critical thinking (CT) affective attributes and cognitive skills are described in this quantitative, descriptive study. The study sample consisted of nurse educators from the National League of Nursing database. The purpose of the study was to gain nursing faculty perception of which teaching strategies…

  6. How to Evaluate a Faculty Governance Structure

    Science.gov (United States)

    Cordes, John W.; Dunbar, David; Gingerich, Jeff

    2013-01-01

    During the 2010-11 academic year, Cabrini College began an evaluation of a faculty governance structure that had been implemented in fall 2007. The processes involved might serve as a roadmap for faculty members and administrators at other institutions who seek to understand the strengths and weaknesses of their governance model and improve shared…

  7. Some LIS Faculty Indicate Reservations about Open Access

    Directory of Open Access Journals (Sweden)

    Richard Hayman

    2016-09-01

    Full Text Available A Review of: Peekhaus, W., & Proferes, N. (2015. How library and information science faculty perceive and engage with open access. Journal of Information Science, 41(5, 640-661. http://dx.doi.org/10.1177/0165551515587855 Objective – To examine the awareness of, attitudes toward, and engagement with open access (OA publishing, based on rank and tenure status among library and information science (LIS faculty in North America. Design – Web-based survey distributed via email. Setting – Accredited library and information science (LIS programs in North America. Subjects – 276 professors and professors emeriti. Methods – Researchers collected email addresses for 1,017 tenure-track, tenured, and emeriti professors from the public websites of the LIS programs. Researchers sent an email invitation to participate in the survey by accessing a URL, with the survey itself delivered using Qualtrics software. The survey included 51 total questions, some with additional sub-questions, and most items used Likert-type rating scale. The researchers analysed the data using SPSS software, and indicated using chi-square tests to measure significance, with a stated intent to get beyond the descriptive statistics commonly seen in other publications. Main Results – This study’s results draw on 276 completed responses, for a response rate of 27%. Researchers reported that 53% of respondents had some experience with publishing in a peer-reviewed OA format. When asked whether they agreed that scholarly articles should be free to access for everyone, pre-tenure assistant professors were most likely to agree (74%, followed by tenured associate professors (62%, full professors (59% and then emeriti professors (8%. However, they found less likelihood that associate professors would have actually published in an OA format, highlighting a “disconnect between beliefs about accessibility of research and actual practice with open access” (p. 646. Researchers also

  8. Burning Out Faculty at Doctoral Research Universities.

    Science.gov (United States)

    Padilla, Miguel A; Thompson, Julia N

    2016-12-01

    The present study examined the importance of time allocation, pressure and support variables together as determinants of faculty burnout. Using a large sample of university faculty (N = 1439), we were able to show that time allocation variables and perceived pressure contribute to faculty burnout. As expected, decreased social support, family, sleep and leisure time were related to higher levels of burnout. Grantsmanship and service activities appeared as the most critical factors associated with faculty burnout. Faculty burnout is an important topic, and the insights provided here help offer some directions for future research as well as the development of effective institutional policies. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

  9. Cognitive dissonance experienced by nurse practitioner faculty.

    Science.gov (United States)

    Fontenot, Holly B; Hawkins, Joellen W; Weiss, Josie A

    2012-08-01

    The purpose of this study was to explicate the concept of cognitive dissonance as experienced and reported by nurse practitioner (NP) faculty members. Responses from NP faculty members to an online survey about their experiences with cognitive dissonance. The respondents detailed their experiences with cognitive dissonance, citing differences between expectations for which they are rewarded and those for which they are paid. Expecting all faculty members to excel in practice, research, teaching, and service may create unrealistic workloads for NP faculty members. Examining expectations and considering creation of a clinical track for faculty who practice may be options administrators of NP programs might explore. ©2012 The Author(s) Journal compilation ©2012 American Academy of Nurse Practitioners.

  10. Faculty Viewpoints on Teaching Quantway®

    Directory of Open Access Journals (Sweden)

    Heather Howington

    2015-01-01

    Full Text Available Quantway is a quantitative reasoning-based pathway for developmental math that has been developed as an alternative to the traditional remedial algebra sequence. To explore the experiences of faculty involved with Quantway, we interviewed eight individuals who have taught the course in the past year to survey their attitudes and opinions about students in their classes, the materials and pedagogies in use, and the collegial interaction of networked faculty. Faculty were selected with the intention of gathering a broad set of opinions resulting from differences of location, experience, and other factors. In this paper, we summarize those interviews by identifying common themes reported by the faculty that highlight strengths and challenges of teaching Quantway. Themes include perceptions about changes in student engagement and attitudes as well as changes in their own mindset; the evolution of teaching strategies and materials used inside and outside the classroom; and the relevance of connections between faculty at different institutions involved in the project.

  11. Planning for Internationalization By Investing in Faculty

    Directory of Open Access Journals (Sweden)

    Lisa K. Childress

    2009-11-01

    Full Text Available Over the last half century, major world events have prompted higher education institutions to develop internationalization plans. In order engage faculty in internationalization, higher education scholars and practitioners have recommended that internationalization plans include allocated resources, such as budgets for academic exchanges, faculty development workshops, and international curricular development and research grants (Olson, Green, & Hill, 2006; Paige, 2005; Siaya & Hayward, 2003. Yet, a frequently cited obstacle to faculty engagement in internationalization plans is lack of funding (Backman, 1984; Bond, 2003; Ellingboe, 1998; Green & Olson, 2003; Steers & Ungsen, 1992; Woolston, 1983. A cross-case analysis reveals that differential investment leads to faculty engagement in internationalization plans. This article discusses how two institutions developed funds from a variety of sources and institutional levels to engage faculty in an institutional planning process. This study offers implications for institutional planning, resource dependency theory, and internationalization.

  12. The Role of Arab Women Faculty in the Public Sphere

    Science.gov (United States)

    Almansour, Sana; Kempner, Ken

    2016-01-01

    This study considers the level of critical involvement women professors in Saudi Arabia have in their university and in the larger society. Princess Nourah Bint Abdulrahman University (PNU), Saudi Arabia, the largest women's university in the world, was the site of this investigation. PNU is the first institution in the Kingdom of Saudi Arabia to…

  13. Discovery: Faculty Publications and Presentations, Fiscal Year 1981

    Science.gov (United States)

    1982-01-01

    bibliography of secondary sources and brief biography . 64 J/Department of English g. ’Werewolves and Vampires in Literature and Film." USAF Academy...overshadow even the most lusty of modem films. Additionally. I pointed out that despite Don Giovanni’s ultimate demise, Mozart does not pass moral

  14. Salary-Trend Study of Faculty in Marketing Management and Research for the Years 1995-96 and 1998-99.

    Science.gov (United States)

    Howe, Richard D.

    This report, covering 2,892 marketing management and research faculty, is part of an annual national survey of faculty salaries. The survey consists of two parts: one covering public and one covering private four-year colleges and universities. Data for the baseline year 1995-96 and the trend year 1998-99 were collected for full-time teaching…

  15. Aspirin induces apoptosis in human leukemia cells independently of NF-kappaB and MAPKs through alteration of the Mcl-1/Noxa balance.

    Science.gov (United States)

    Iglesias-Serret, Daniel; Piqué, Maria; Barragán, Montserrat; Cosialls, Ana M; Santidrián, Antonio F; González-Gironès, Diana M; Coll-Mulet, Llorenç; de Frias, Mercè; Pons, Gabriel; Gil, Joan

    2010-02-01

    Aspirin and other non-steroidal anti-inflammatory drugs induce apoptosis in most cell types. In this study we examined the mechanism of aspirin-induced apoptosis in human leukemia cells. We analyzed the role of nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinases (MAPKs) pathways. Furthermore, we studied the changes induced by aspirin in some genes involved in the control of apoptosis at mRNA level, by performing reverse transcriptase multiplex ligation-dependent probe amplification (RT-MLPA), and at protein level by Western blot. Our results show that aspirin induced apoptosis in leukemia Jurkat T cells independently of NF-kappaB. Although aspirin induced p38 MAPK and c-Jun N-terminal kinase activation, selective inhibitors of these kinases did not inhibit aspirin-induced apoptosis. We studied the regulation of Bcl-2 family members in aspirin-induced apoptosis. Aspirin increased the mRNA levels of some pro-apoptotic members, such as BIM, NOXA, BMF or PUMA, but their protein levels did not change. In contrast, aspirin decreased the protein levels of Mcl-1. Interestingly, in the presence of aspirin the protein levels of Noxa remained high. This alteration of the Mcl-1/Noxa balance was also found in other leukemia cell lines and primary chronic lymphocytic leukemia cells (CLL). Furthermore, in CLL cells aspirin induced an increase in the protein levels of Noxa. Knockdown of Noxa or Puma significantly attenuated aspirin-induced apoptosis. These results indicate that aspirin induces apoptosis through alteration of the Mcl-1/ Noxa balance.

  16. The expression of bcl-xL and mcl-1 following acquired chemoresistance in pancreatic cancer cells%胰腺癌细胞对5-Fu和吉西它滨获得性耐药后bcl-xL和mcl-1表达的变化

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Objective:To study the relationships between the acquired resistance of Gemcitabine and 5-Fu and the expression of bcl-xL and mcl-1 in pancreatic cancer cell lines.Methods:The cytotoxic effects of 5-Fu and Gemcitabine in Panc-1,Mia-Paca-2 and Capan-1 pancreatic cancer cells were assessed using Sulforhodamine B methods and expression levels of anti-apoptotic genes of the bcl-xL and mcl-1 were analyzed by Western blot.Results:5-Fu and Gemcitabine displayed cytotoxic effects towards three pancreatic cancer cell lines.After recurrent treatment with 5-Fu,the IC50 values in Capan-1 cells increased 2.1 fold compared to their parental cells (P<0.05).Following recurrent treatment with Gemcitabine,the IC50 values in Capan-1 cells increased 1.5 fold (P<0.05).Western blot analysis show that aftter recurrent treatment with 5-Fu and Gemcitabine the expression of bcl-xL and mcl-1 upgraded.Conclusion:Overexpression of bcl-xL and mcl-1 following recurrent exposure to 5-Fu or Gemcitabine are associaed with the acqurired these drugs.%目的:探讨胰腺癌细胞对5-Fu和吉西它滨的获得性耐药后bcl-xL和mcl-1表达的变化.方法:应用SRB方法检测5-Fu和吉西它滨对胰腺癌细胞株Panc-1,Mia-Paca-2和Capan-1的细胞毒性作用,应用Western blot法来检测bcl-xL和mcl-1的蛋白表达水平.结果:5-Fu和吉西它滨对3株胰腺癌细胞均产生了细胞毒性作用, 5-Fu长期作用后, Capan-1细胞IC50上升了2.1倍(P<0.05).吉西它滨长期作用后,Capan-1细胞IC50上升了1.5倍(P<0.05).5-Fu或吉西它滨长期作用后,产生了获得性耐药的胰腺癌细胞bcl-xL和mcl-1表达水平上调.结论:5-Fu或吉西它滨长期作用后,产生了获得性耐药,这种耐药与bcl-xL和mcl-1的过度表达相关.

  17. Exploration of a leadership competency model for medical school faculties in Korea.

    Science.gov (United States)

    Lee, Yong Seok; Oh, Dong Keun; Kim, Myungun; Lee, Yoon Seong; Shin, Jwa Seop

    2010-12-01

    To adapt to rapid and turbulent changes in the field of medicine, education, and society, medical school faculties need appropriate leadership. To develop leadership competencies through education, coaching, and mentoring, we need a leadership competency model. The purpose of this study was to develop a new leadership competency model that is suitable for medical school faculties in Korea. To collect behavioral episodes with regard to leadership, we interviewed 54 subjects (faculties, residents, nurses) and surveyed 41 faculties with open-ended questionnaires. We classified the behavioral episodes based on Quinn and Cameron's leadership competency model and developed a Likert scale questionnaire to perform a confirmatory factor analysis. Two hundred seven medical school faculties responded to the questionnaire. The competency clusters that were identified by factor analysis were professionalism, citizenship, leadership, and membership to an organization. Accordingly, each cluster was linked with a dimension: self, society, team (that he/she is leading), and organization (to which he/she belongs). The clusters of competencies were: professional ability, ethics/morality, self-management, self-development, and passion; public interest, networking, social participation, and active service; motivating, caring, promoting teamwork, nurturing, conflict management, directing, performance management, and systems thinking; organizational orientation, collaboration, voluntary participation, and cost-benefit orientation. This competency model that fits medical school faculties in Korea can be used to design and develop selection plans, education programs, feedback tools, diagnostic evaluation tools, and career plan support programs.

  18. Examining Workplace Ostracism Experiences in Academia: Understanding How Differences in the Faculty Ranks Influence Inclusive Climates on Campus.

    Science.gov (United States)

    Zimmerman, Carla A; Carter-Sowell, Adrienne R; Xu, Xiaohong

    2016-01-01

    Research on the retention of women in academia has focused on challenges, including a "chilly climate," devaluation, and incivility. The unique consequences of workplace ostracism - being ignored and excluded by others in an organizational setting - require focus on this experience as another interpersonal challenge for women in academia. The purpose of this study is to examine differences in the faculty experiences and outcomes of workplace ostracism, and to determine if these experiences are affected significantly by the gender composition of an employee's specific department. Participants were recruited at two time points to complete campus climate surveys that were distributed to faculty at a large, public, research university. We examined the number of reported ostracism experiences (Study 1) and perceived information sharing (Study 2) among male and female university faculty. The findings indicated that female faculty members perceived more workplace ostracism than male faculty members. Analyses of department gender ratios suggested that the proportion of women in the department did not reduce the amount of workplace ostracism experienced by women. No gender differences were found in perceived information sharing. However, we found that Faculty of Color, both men and women, reported more frequent information exclusion than White faculty. These results have important implications for theoretical and practical understandings of workplace demography and suggest that it is necessary to look at subtle, ambiguous forms of discrimination in order to increase retention of faculty from underrepresented groups in academia.

  19. Examining Workplace Ostracism Experiences in Academia: Understanding How Differences in the Faculty Ranks Influence Inclusive Climates on Campus

    Directory of Open Access Journals (Sweden)

    Carla A. Zimmerman

    2016-05-01

    Full Text Available Research on the retention of women in academia has focused on challenges, including a chilly climate, devaluation, and incivility. The unique consequences of workplace ostracism – being ignored and excluded by others in an organizational setting – require focus on this experience as another interpersonal challenge for women in academia. The purpose of this study is to examine differences in the faculty experiences and outcomes of workplace ostracism, and to determine if these experiences are affected significantly by the gender composition of an employee’s specific department. Participants were recruited at two time points to complete campus climate surveys that were distributed to faculty at a large, public, research university. We examined the number of reported ostracism experiences (Study 1 and perceived information sharing (Study 2 among male and female university faculty. The findings indicated that female faculty members perceived more workplace ostracism than male faculty members. Analyses of department gender ratios suggested that the proportion of women in the department did not reduce the amount of workplace ostracism experienced by women. No gender differences were found in perceived information sharing. However, we found that Faculty of Color, both men and women, reported more frequent information exclusion than White faculty. These results have important implications for theoretical and practical understandings of workplace demography and suggest that it is necessary to look at subtle, ambiguous forms of discrimination in order to increase retention of faculty from underrepresented groups in academia.

  20. 基于蒙特卡罗的无线传感器网络战场目标定位模型%Wireless Sensor Network Battlefield Target Fixed Position Model Based on MCL

    Institute of Scientific and Technical Information of China (English)

    唐克; 谢保军; 卢金星

    2011-01-01

    针对目前我军使用GPS全球定位系统存在的不足,在对无线传感器网络进行分析的基础上,建立基于蒙特卡罗(Monte Carlo,MCL)的无线传感器网络定位算法模型.分别对MCL、质心和Amorphous 3种算法进行比较,并以蒙特卡罗定位算法为例,研究估计误差与网络参数及算法间的关系.结果表明,MCL算法误差最小.%Aiming at the current shortcomings of military use of global positioning system (GPS), based on analyzing wireless sensor network, establish wireless sensor network positioning algorithm based on Monte Carlo (MCL). Compare MCL algorithm, centroid algorithm, and Amorphous algorithm. Take MCL positioning algorithm as example, research and estimate the relationship among error, network parameter and algorithm. The result shows that MCL algorithm's error is smallest.

  1. Graduate programs in health administration: faculty academic reputation and faculty research reputation by program location and program reputation.

    Science.gov (United States)

    Nowicki, M

    1995-01-01

    This study used program location and program reputation to describe two important faculty characteristics: academic reputation and research reputation. The study involved 44 graduate programs in health administration representing four program locations: schools of public health, business, medicine/allied health, and graduate/independent. Fourteen programs were identified as ranked programs and the remaining 30 programs were identified as unranked programs. While the study identifies many differences, few are significant, thus adding credence to the argument for diversity in program location and diminishing credence in the argument for program reputation.

  2. Motivational issues of faculty in Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Akram AbdulCader

    2014-12-01

    Full Text Available This study focused on the factors that affect motivation of faculty in Saudi Arabia. It included two surveys and open-ended queries to a focus group of five academic managers and 25 faculty members of varying nationalities, rank, and institutes in Saudi Arabia. The research showed that the faculties in Saudi Arabia’s higher education industry feel disconnected from the program development. The faculty members did not feel motivated to participate in the development and improvement of the academic program due to: (a lack of monetary and non-monetary incentives, (b management not involving faculty in decision-making, and (c lack of recognition and moral support. However, the faculties were intrinsically motivated to perform their best within the confines of the classroom. The results of the study indicated that there was a greater interest in intrinsic motivation as a personal measure for success inside the classroom, but extrinsic motivation was a factor that needed greater improvement from the management of the universities for faculty to partake in development of the program. DOI:  10.18870/hlrc.v4i4.211

  3. Faculty and student perceptions of the feasibility of individual student-faculty meetings.

    Science.gov (United States)

    Mulder, B F; Erich, M H; Borleffs, J C C; Elgersma, A F; Cohen-Schotanus, J

    2012-05-01

    The extent to which students feel involved in their education positively influences academic achievement. Individual student-faculty meetings can foster student involvement. To be effective, faculty acknowledgement of the benefit of these meetings is a prerequisite. The aim of this study was to explore faculty perceptions of individual student-faculty meetings. In addition we investigated students' perceptions. As part of the undergraduate programme, mandatory individual intake and follow-up meetings between first-year medical students (n = 425) and senior faculty members (n = 34) have been implemented from 2009 onwards. We administered a questionnaire on faculty perceptions of the benefit and impact of intake meetings. Subsequently, after both meetings had been held, strong and weak points of the mandatory programme were explored using open-ended questions. Students' perceptions were investigated by open-ended questions as a part of the curriculum evaluation process. Faculty enjoyed the meetings (90 %), perceived the meetings to be beneficial (74 %) and expected a positive effect on student involvement (74 %). Faculty appreciated the opportunity to give advice tailored to students' personal needs and levels of performance. The students appreciated the meetings and the attention given to their personal situation and study progress. Faculty and student appreciation of the meetings seems to support the assumption that the individual meetings increase students' social and academic involvement. Further research should focus on the impact of individual student-faculty meetings on students' learning behaviours.

  4. WHERE Are the Faculty? Fulfilling the Traditional Faculty Role at a Distance.

    Science.gov (United States)

    Wood, Felecia G

    2016-01-01

    Innovative approaches are needed to retain seasoned nursing faculty. Technology provides opportunities for faculty to fulfill the traditional roles of teaching, research, and service from a site removed from the traditional campus. The purpose of this article is to encourage faculty and administrators in traditional, land-based colleges and universities to thoughtfully consider the advantages and challenges of the remote worksite for faculty based on the experience of one faculty. Some faculty are better suited to a remote work environment than others. Long-term established faculty may be better able to successfully transition to the tripartite faculty roles with greater ease than novice nurse educators as a result of their familiarity with the institutional resources and comfort in the teaching role. Preparation for the remote experience must be diligent and thoughtful, considering equipment needs, connectivity, and support personnel and strategies for ensuring continued engagement within the nursing education program. Institutional policies must also be considered related to fulfillment of the faculty role via distance technology. A pilot experience for one faculty, as described here, may be useful for evaluating the cost-benefit to the individual and the institution.

  5. The full-time clinical faculty: what goes around, comes around.

    Science.gov (United States)

    Barzansky, Barbara; Kenagy, Gretchen

    2010-02-01

    In his 1910 report entitled Medical Education in the United States and Canada, Abraham Flexner advanced an ideal model of medical education that included a university-based, full-time, salaried faculty whose time was devoted to teaching and research. This article traces the evolution of the "full-time" concept for clinical faculty and describes factors that have affected its implementation. Between 1910 and the 1930s, the full-time system for clinical faculty was implemented at a limited number of medical schools, but lack of financing made the system generally unworkable. The implementation of the "geographic" full-time concept during the 1940s to 1960s allowed faculty to be considered full-time while earning much of their income from clinical practice. Even then, there were concerns that medical schools would bring pressure on such faculty to increase their clinical activity for the purpose of supporting the institution. After the rise of private and public payers, clinical practice income came to be an explicit and increasingly important source of medical school revenue. This stimulated a significant expansion in the number of full-time clinical faculty over the next 40 years. In the 100 years following the Flexner Report, clinical faculty became "full-time" and "salaried," but not in the way Flexner imagined. Instead of deriving their salaries from the resources of the medical school, they are significantly contributing to institutional financing through their practice. Flexner's concern about the "distraction" of clinical practice interfering with faculty participation in education has come full circle, remaining a primary issue in medical education today.

  6. Can Tablet Computers Enhance Faculty Teaching?

    Science.gov (United States)

    Narayan, Aditee P; Whicker, Shari A; Benjamin, Robert W; Hawley, Jeffrey; McGann, Kathleen A

    2015-06-01

    Learner benefits of tablet computer use have been demonstrated, yet there is little evidence regarding faculty tablet use for teaching. Our study sought to determine if supplying faculty with tablet computers and peer mentoring provided benefits to learners and faculty beyond that of non-tablet-based teaching modalities. We provided faculty with tablet computers and three 2-hour peer-mentoring workshops on tablet-based teaching. Faculty used tablets to teach, in addition to their current, non-tablet-based methods. Presurveys, postsurveys, and monthly faculty surveys assessed feasibility, utilization, and comparisons to current modalities. Learner surveys assessed perceived effectiveness and comparisons to current modalities. All feedback received from open-ended questions was reviewed by the authors and organized into categories. Of 15 eligible faculty, 14 participated. Each participant attended at least 2 of the 3 workshops, with 10 to 12 participants at each workshop. All participants found the workshops useful, and reported that the new tablet-based teaching modality added value beyond that of current teaching methods. Respondents developed the following tablet-based outputs: presentations, photo galleries, evaluation tools, and online modules. Of the outputs, 60% were used in the ambulatory clinics, 33% in intensive care unit bedside teaching rounds, and 7% in inpatient medical unit bedside teaching rounds. Learners reported that common benefits of tablet computers were: improved access/convenience (41%), improved interactive learning (38%), and improved bedside teaching and patient care (13%). A common barrier faculty identified was inconsistent wireless access (14%), while no barriers were identified by the majority of learners. Providing faculty with tablet computers and having peer-mentoring workshops to discuss their use was feasible and added value.

  7. Latina Faculty Transcending Barriers: Peer Mentoring in a Hispanic-Serving Institution

    Science.gov (United States)

    Murakami, Elizabeth T.; Núñez, Anne-Marie

    2014-01-01

    In this article, the authors conducted a research metasynthesis of publications by a group of Latina tenure-track faculty participating in a peer mentoring group, the Research for the Educational Advancement of Latin@s (REAL) collaborative, housed in one Hispanic Serving Institution. Due to the small representation of Latinas in the academy, the…

  8. A Survey of Physical Sciences, Engineering and Mathematics Faculty Regarding Author Fees in Open Access Journals

    Science.gov (United States)

    Cusker, Jeremy; Rauh, Anne E.

    2014-01-01

    Discussions of the potential of open access publishing frequently must contend with the skepticism of research authors regarding the need to pay author fees (also known as publication fees). With that in mind, the authors undertook a survey of faculty, postdocs, and graduate students in physical science, mathematics, and engineering fields at two…

  9. Work-Life: Policy and Practice Impacting LG Faculty and Staff in Higher Education

    Science.gov (United States)

    Munn, Sunny L.; Hornsby, Eunice Ellen

    2008-01-01

    The work-life policies and benefits practices of public universities and the extent to which lesbian and gay (LG) faculty, staff and families receive different work-life benefits than their heterosexual married counterparts are examined. The analysis was conducted by searching university work-life benefits websites. Major benefits for domestic…

  10. Opportunities for Faculty to Influence Academic Matters at Kazakh National University and Eurasian National University

    Science.gov (United States)

    Sarinzhipov, Aslan

    2013-01-01

    Kazakhstan's higher education system is based on the Soviet governance structure, limited academic freedom and no autonomy from the state. In such a system faculties are contract employees delivering predesigned courses with no incentive to bring new ideas and methods. But employers and the general public are concerned with the mismatch between…

  11. PR on Campus: Educating Your Faculty and Staff about Your PR Shop Pays Dividends.

    Science.gov (United States)

    Snyder, Peggy

    1984-01-01

    An effective first step toward creating new media opportunities is internal education. These include encouraging top college administrators to involve the public relations department in pre-planning; increasing access to newsworthy, appropriately targeted stories; and educating faculty about effective media relations. (MLW)

  12. A Survey of Physical Sciences, Engineering and Mathematics Faculty Regarding Author Fees in Open Access Journals

    Science.gov (United States)

    Cusker, Jeremy; Rauh, Anne E.

    2014-01-01

    Discussions of the potential of open access publishing frequently must contend with the skepticism of research authors regarding the need to pay author fees (also known as publication fees). With that in mind, the authors undertook a survey of faculty, postdocs, and graduate students in physical science, mathematics, and engineering fields at two…

  13. Instructional Practices in Introductory Geoscience Courses: Results of a National Faculty Survey

    Science.gov (United States)

    MacDonald, R.; Manduca, C. A.; Mogk, D. W.; Tewksbury, B. J.

    2004-12-01

    teaching methods from a wide variety of sources, they rely more heavily on discussions with their colleagues for information about teaching methods. This description of current teaching practices provides a benchmark against which we can monitor how instruction in introductory courses responds to increased understanding of student learning as well as to broad sharing of expertise among geoscience faculty through professional meetings, publications, workshops, and new on-line resources.

  14. Roscovitine-induced apoptosis in neutrophils and neutrophil progenitors is regulated by the Bcl-2-family members Bim, Puma, Noxa and Mcl-1.

    Directory of Open Access Journals (Sweden)

    Sanjivan Gautam

    Full Text Available Neutrophil granulocyte (neutrophil apoptosis plays a key role in determining inflammation in infectious and non-infectious settings. Recent work has shown that inhibitors of cyclin-dependent kinases (cdk such as roscovitine can potently induce neutrophil apoptosis and reduce inflammation. Using a conditional Hoxb8-expression system we tested the participation of Bcl-2-family proteins to roscovitine-induced apoptosis in mouse neutrophils and in neutrophil progenitor cells. Bcl-2 strongly protected against roscovitine-induced apoptosis in neutrophils. The isolated loss of either Bim or noxa provided significant, partial protection while protection through combined loss of Bim and noxa or Bim and Puma was only slightly greater than this individual loss. The only substantial change in protein levels observed was the loss of Mcl-1, which was not transcriptional and was inhibited by proteasome blockade. In progenitor cells there was no protection by the loss of Bim alone but substantial protection by the loss of both Bim and Puma; surprisingly, strongest protection was seen by the isolated loss of noxa. The pattern of protein expression and Mcl-1-regulation in progenitor cells was very similar to the one observed in differentiated neutrophils. In addition, roscovitine strongly inhibited proliferation in progenitor cells, associated with an accumulation of cells in G2/M-phase.

  15. Roscovitine-induced apoptosis in neutrophils and neutrophil progenitors is regulated by the Bcl-2-family members Bim, Puma, Noxa and Mcl-1.

    Science.gov (United States)

    Gautam, Sanjivan; Kirschnek, Susanne; Wiesmeier, Michael; Vier, Juliane; Häcker, Georg

    2013-01-01

    Neutrophil granulocyte (neutrophil) apoptosis plays a key role in determining inflammation in infectious and non-infectious settings. Recent work has shown that inhibitors of cyclin-dependent kinases (cdk) such as roscovitine can potently induce neutrophil apoptosis and reduce inflammation. Using a conditional Hoxb8-expression system we tested the participation of Bcl-2-family proteins to roscovitine-induced apoptosis in mouse neutrophils and in neutrophil progenitor cells. Bcl-2 strongly protected against roscovitine-induced apoptosis in neutrophils. The isolated loss of either Bim or noxa provided significant, partial protection while protection through combined loss of Bim and noxa or Bim and Puma was only slightly greater than this individual loss. The only substantial change in protein levels observed was the loss of Mcl-1, which was not transcriptional and was inhibited by proteasome blockade. In progenitor cells there was no protection by the loss of Bim alone but substantial protection by the loss of both Bim and Puma; surprisingly, strongest protection was seen by the isolated loss of noxa. The pattern of protein expression and Mcl-1-regulation in progenitor cells was very similar to the one observed in differentiated neutrophils. In addition, roscovitine strongly inhibited proliferation in progenitor cells, associated with an accumulation of cells in G2/M-phase.

  16. Examining Community-Engaged Scholarship in Public Administration Programs

    Science.gov (United States)

    Norvell, Katrina Herndon

    2010-01-01

    This study sought to broaden the understanding of the role that academic professions play in shaping the values and attitudes of faculty toward CES. This study explored faculty perceptions regarding the factors that encourage or dissuade them in the pursuit of CES within public administration programs. As a framework for research, a conceptual…

  17. Mathematics Turned Inside Out: The Intensive Faculty Versus the Extensive Faculty

    CERN Document Server

    Grcar, Joseph F

    2011-01-01

    Research universities in the United States have larger mathematics faculties outside their mathematics departments than inside. Members of this "extensive" faculty conduct most mathematics research, their interests are the most heavily published areas of mathematics, and they teach this mathematics in upper division courses independent of mathematics departments. The existence of this de facto faculty challenges the pertinence of institutional and national policies for higher education in mathematics, and of philosophical and sociological studies of mathematics that are limited to mathematics departments alone.

  18. Faculty Rights to Courses and Digital Courseware

    Science.gov (United States)

    Ahmadi, Shafiqa

    2017-01-01

    Ownership of traditional courseware is vested in the faculty. In the digital forum, however, under the Copyright Act of 1976, case law, and institutional policy ownership may be vested in the institution.

  19. Creating Networks through Interinstitutional Faculty Collaboration.

    Science.gov (United States)

    Marino, Sarah R.

    2002-01-01

    Describes efforts by the consortium Associated Colleges of the Midwest to support interinstitutional faculty collaboration and development. Focuses on three programs: the Global Partners Project, an information literacy grant, and an academic collaboration grant. (EV)

  20. Technology Adoption in Higher Education: Overcoming Anxiety through Faculty Bootcamp

    Science.gov (United States)

    Johnson, Terri; Wisniewski, Mary Ann; Kuhlemeyer, Greg; Isaacs, Gerald; Krzykowski, Jamie

    2012-01-01

    The reluctance to design and teach online courses in higher education is often attributed to technology anxiety in faculty. This article documents a faculty development model that has successfully helped faculty overcome this obstacle. "Bootcamps," faculty development programs held at Carroll University in Waukesha, WI, were specifically and…

  1. To Heaven or Hell: Sensemaking about Why Faculty Leave

    Science.gov (United States)

    O'Meara, KerryAnn; Lounder, Andrew; Campbell, Corbin M.

    2014-01-01

    This article analyzes sensemaking about faculty departure among administrators, faculty colleagues, and faculty leavers in one research university. A mixed methods database was analyzed to reveal four dominant explanations for faculty departure and two influences on sensemaking. Dominant explanations included better opportunities, the likelihood…

  2. Faculty Perception of Support to Do Their Job Well

    Science.gov (United States)

    Eaton, Charissa K.; Osgood, Aurea K.; Cigrand, Dawnette L.; Dunbar, Ann-Marie L.

    2015-01-01

    Research has commonly suggested that adequate and appropriate mentoring and faculty perception of support for a work-life balance are important factors in the recruitment, development, and retention of university faculty. To better understand the role of these factors in faculty job performance at teaching universities, faculty from such a…

  3. New Faculty: Catalyst for Change in Academic Departments.

    Science.gov (United States)

    Naidoo, Kogi

    This paper discusses a faculty development program for new faculty at the M L Sultan Technikon in Durban, South Africa, especially as it relates to faculty development programs at other South African institutions. This associate lecturer training program was designed to provide support and training for newly appointed black faculty who did not…

  4. Factors Affecting the Occurrence of Faculty-Doctoral Student Coauthorship

    Science.gov (United States)

    Maher, Michelle A.; Timmerman, Briana Crotwell; Feldon, David F.; Strickland, Denise

    2013-01-01

    Using faculty narratives, this study identifies factors affecting the occurrence of faculty-doctoral student coauthorship. Norms of the discipline, resources, faculty goals for students, faculty goals for themselves, and institutional expectations emerged as dominant factors. Each factor is explored separately and as part of an interlocking…

  5. Gender Differences in Business Faculty's Research Motivation

    Science.gov (United States)

    Chen, Yining; Zhao, Qin

    2013-01-01

    The authors use expectancy theory to evaluate gender differences in key factors that motivate faculty to conduct research. Using faculty survey data collected from 320 faculty members at 10 business schools, they found that faculty members, both men and women, who displayed higher motivation were more productive in research. Among them, pretenured…

  6. Comparison of Sports Sciences and Education Faculty Students' Aggression Scores

    Science.gov (United States)

    Atan, Tülin

    2016-01-01

    The aim of this study was to compare the aggression scores of Sports Sciences Faculty and Education Faculty students and also to examine the effects of some demographic variables on aggression. Two hundred Sports Sciences Faculty students (who engage in sporting activities four days a week for two hours) and 200 Education Faculty students (who do…

  7. Disrupting Faculty Service: Using Technology to Increase Academic Service Productivity

    Science.gov (United States)

    Burnett, Perry; Shemroske, Kenneth; Khayum, Mohammed

    2014-01-01

    Scholarly attention regarding faculty involvement has primarily focused on faculty opinions of shared governance and faculty influence on institutional decision-making. There has been limited attention given to academic service productivity and the effectiveness of traditional approaches toward the accomplishment of faculty service requirements.…

  8. Comparison of differences in performance evaluation of faculty by students with faculty’s self-assessment

    Directory of Open Access Journals (Sweden)

    KOUROSH AZIZI

    2014-07-01

    Full Text Available Introduction: The present study aimed to compare self-assessment forms of coursework taught in the school of public health at undergraduate, graduate, and postgraduate levels and students’ evaluation of the performance of the faculty members at these levels. Methods: The subjects in this cross-sectional study were the faculty members and students of the School of Public Health and Nutrition, Shiraz University of Medical Sciences, Shiraz, Iran. The data were collected using a sociodemographic information form and evaluation forms of professors prepared by the Educational Development Center (EDC. The faculty members were assessed by the students in undergraduate and graduate classes. Among the study subjects, 23 faculty members filled out the self-assessment forms which were then evaluated by 23 students. Then, the data were analyzed using the SPSS 14. Paired t-test was used to compare the students’ evaluation of the faculty members’ performance and the professors’ self-assessment. Results: The mean score of self-assessment of the faculty members who taught undergraduate courses was 289.7+8.3, while that of the students’ evaluation was 281.3+16.1; the difference was statistically significant (t=3.56, p=0.001. Besides, the mean score of the self-assessment of the faculty members who taught graduate courses was 269.0+9.7, while that of the students’ evaluation was 265.7+14.6 but the difference was not statistically significant (t=1.09, p=0.28. Conclusion: Teaching performance perceptions of the faculty were similar to those of the graduate students as compared to the undergraduate ones. This may reflect better understanding of coursework at this level compared to the undergraduate students. Faculty members may need to adjust teaching methods to improve students’ performance and understanding especially in the undergraduate level.

  9. The Faculty Self-Reported Assessment Survey (FRAS): differentiating faculty knowledge and experience in assessment.

    Science.gov (United States)

    Hanauer, David I; Bauerle, Cynthia

    2015-01-01

    Science, technology, engineering, and mathematics education reform efforts have called for widespread adoption of evidence-based teaching in which faculty members attend to student outcomes through assessment practice. Awareness about the importance of assessment has illuminated the need to understand what faculty members know and how they engage with assessment knowledge and practice. The Faculty Self-Reported Assessment Survey (FRAS) is a new instrument for evaluating science faculty assessment knowledge and experience. Instrument validation was composed of two distinct studies: an empirical evaluation of the psychometric properties of the FRAS and a comparative known-groups validation to explore the ability of the FRAS to differentiate levels of faculty assessment experience. The FRAS was found to be highly reliable (α = 0.96). The dimensionality of the instrument enabled distinction of assessment knowledge into categories of program design, instrumentation, and validation. In the known-groups validation, the FRAS distinguished between faculty groups with differing levels of assessment experience. Faculty members with formal assessment experience self-reported higher levels of familiarity with assessment terms, higher frequencies of assessment activity, increased confidence in conducting assessment, and more positive attitudes toward assessment than faculty members who were novices in assessment. These results suggest that the FRAS can reliably and validly differentiate levels of expertise in faculty knowledge of assessment.

  10. Marshall Space Flight Center Faculty Fellowship Program

    Science.gov (United States)

    Six, N. F. (Compiler)

    2015-01-01

    The Faculty Fellowship program was revived in the summer of 2015 at NASA Marshall Space Flight Center, following a period of diminished faculty research activity here since 2006 when budget cuts in the Headquarters' Education Office required realignment. Several senior Marshall managers recognized the need to involve the Nation's academic research talent in NASA's missions and projects to the benefit of both entities. These managers invested their funds required to establish the renewed Faculty Fellowship program in 2015, a 10-week residential research involvement of 16 faculty in the laboratories and offices at Marshall. These faculty engineers and scientists worked with NASA collaborators on NASA projects, bringing new perspectives and solutions to bear. This Technical Memorandum is a compilation of the research reports of the 2015 Marshall Faculty Fellowship program, along with the Program Announcement (appendix A) and the Program Description (appendix B). The research touched on seven areas-propulsion, materials, instrumentation, fluid dynamics, human factors, control systems, and astrophysics. The propulsion studies included green propellants, gas bubble dynamics, and simulations of fluid and thermal transients. The materials investigations involved sandwich structures in composites, plug and friction stir welding, and additive manufacturing, including both strength characterization and thermosets curing in space. The instrumentation projects involved spectral interfero- metry, emissivity, and strain sensing in structures. The fluid dynamics project studied the water hammer effect. The human factors project investigated the requirements for close proximity operations in confined spaces. Another team proposed a controls system for small launch vehicles, while in astrophysics, one faculty researcher estimated the practicality of weather modification by blocking the Sun's insolation, and another found evidence in satellite data of the detection of a warm

  11. The Faculty Web Page: Contrivance or Continuation?

    Science.gov (United States)

    Lennex, Lesia

    2007-01-01

    In an age of Internet education, what does it mean for a tenure/tenure-track faculty to have a web page? How many professors have web pages? If they have a page, what does it look like? Do they really need a web page at all? Many universities have faculty web pages. What do those collective pages look like? In what way do they represent the…

  12. 90y-Ibritumumab Tiuxetan (Zevalin®-BEAM/C with Autologous Stem Cell Support as Therapy for Advanced Mantle Cell Lymphoma. - Preliminary Results From the Third Nordic II Study (MCL3)

    DEFF Research Database (Denmark)

    Kolstad, Arne; Laurell, Anna; Andersen, Niels S

    The Nordic Lymphoma Group has since 1996 conducted three consecutive phase II trials for front-line treatment of MCL patients ≤ 65 years of age. The first protocol (MCL1) 1996-2000 introduced high-dose chemotherapy with autologous stem cell support (unpurged or ex vivo purged) as consolidation...... with alternating cycles of maxi-CHOP-rituximab (3 cycles) and Ara-C-rituximab (3 cycles). Response evaluation was done after cycle 5. PET/CT was recommended, but could not influence the response evaluation, which was done according to the International Workshop criteria. Responders underwent in vivo purged harvest...

  13. Publicity and public relations

    Science.gov (United States)

    Fosha, Charles E.

    1990-01-01

    This paper addresses approaches to using publicity and public relations to meet the goals of the NASA Space Grant College. Methods universities and colleges can use to publicize space activities are presented.

  14. The CXCR4 inhibitor BL-8040 induces the apoptosis of AML blasts by downregulating ERK, BCL-2, MCL-1 and cyclin-D1 via altered miR-15a/16-1 expression.

    Science.gov (United States)

    Abraham, M; Klein, S; Bulvik, B; Wald, H; Weiss, I D; Olam, D; Weiss, L; Beider, K; Eizenberg, O; Wald, O; Galun, E; Avigdor, A; Benjamini, O; Nagler, A; Pereg, Y; Tavor, S; Peled, A

    2017-04-07

    CXCR4 is a key player in the retention and survival of human acute myeloid leukemia (AML) blasts in the bone marrow (BM) microenvironment. We studied the effects of the CXCR4 antagonist BL-8040 on the survival of AML blasts, and investigated the molecular mechanisms by which CXCR4 signaling inhibition leads to leukemic cell death. Treatment with BL-8040 induced the robust mobilization of AML blasts from the BM. In addition, AML cells exposed to BL-8040 underwent differentiation. Furthermore, BL-8040 induced the apoptosis of AML cells in vitro and in vivo. This apoptosis was mediated by the upregulation of miR-15a/miR-16-1, resulting in downregulation of the target genes BCL-2, MCL-1 and cyclin-D1. Overexpression of miR-15a/miR-16-1 directly induced leukemic cell death. BL-8040-induced apoptosis was also mediated by the inhibition of survival signals via the AKT/ERK pathways. Importantly, treatment with a BCL-2 inhibitor induced apoptosis and act together with BL-8040 to enhance cell death. BL-8040 also synergized with FLT3 inhibitors to induce AML cell death. Importantly, this combined treatment prolonged the survival of tumor-bearing mice and reduced minimal residual disease in vivo. Our results provide a rationale to test combination therapies employing BL-8040 and BCL-2 or FLT3 inhibitors to achieve increased efficacy of these agents.Leukemia advance online publication, 7 April 2017; doi:10.1038/leu.2017.82.

  15. A Relational Study of Principal Leadership Styles, Faculty Morale, and Faculty Job Satisfaction at Selected Elementary Schools

    Science.gov (United States)

    Ramsey Hearn, Dawn Vyola

    2013-01-01

    The purpose of this study was to determine if a significant relationship existed between principal leadership styles, faculty morale, and faculty job satisfaction at selected elementary schools. Specifically, the study examined if the perception teachers had of their principals. leadership styles had an impact on faculty morale and faculty job…

  16. The Relationship between Faculty Involvement in Governance and Faculty Vitality: The Case of North Carolina Community Colleges

    Science.gov (United States)

    Madray, Van

    2009-01-01

    This study examines the effects of governance involvement on the vitality of community college faculty members. This study explores the degree to which involvement in the governance of a college through a faculty senate fosters the vitality of elected faculty members. While faculty vitality is a difficult concept to measure directly, faculty…

  17. Creating a healthy work environment for nursing faculty.

    Science.gov (United States)

    Kuehn, Mary Beth

    2010-01-01

    Nursing administrators and faculty have a professional and ethical responsibility to develop and maintain a caring and healthy work environment for nursing faculty. To recruit and retain quality nursing faculty in the current nursing faculty shortage, a healthy work environment is essential. This article focuses on nursing administrators' and nursing faculty members' role in promoting a healthy academic work environment. Strategies to develop and sustain this environment are discussed.

  18. Lobbying for Contingent Faculty Interests

    Science.gov (United States)

    Byrd, Nicole

    2008-01-01

    Lobbying has become a dirty word thanks to well-publicized cases involving lobbyists such as Jack Abramoff. However, advocating for one's positions is a positive act of civic engagement. The right to petition the government is enshrined in the Constitution, and lawmakers depend on interaction with constituents to know what the people they…

  19. Attitude of Indian dental professionals toward scientific publications: A questionnaire based study.

    Science.gov (United States)

    Verma, Pradhuman; Sachdeva, Suresh K; Verma, Kanika Gupta; Khosa, Rameen; Basavraju, Suman; Dutta, Sanjay

    2015-08-01

    Due to competitiveness and academic benefits, most dental professionals feel an urgent need to increase their publications. Hence, we explored the attitude of students and faculty members toward scientific publications through a questionnaire. A questionnaire consisting of 13 questions was sent by e-mails and posting the printed copies to dental postgraduate (PG) students (second and third year) and faculty members (n = 500 each). The returned completed questionnaires were analyzed. About 37% of dental PG faculty and 35.6% PG students responded to the questionnaire, with overall response of 72.6%. Among the PG faculty, professors (P) had more scientific publications, followed by senior lecturers (SL) and readers (R). The publications as first or corresponding author were less among both faculty and PG students while co-authorship was more among PG students compared to faculty members. Awareness about the term "plagiarism" was overall high and relatively highest among R, followed by SL, P and PG students. The percentage of publications in fee charging journals was more among PG students than faculty members and self-funding for publication was observed in 86.4% of PG students and 94-100% among faculty members. About 72.6% of dental professionals were involved in publishing of their research work and the number of publications increased steadily with an increase in their academic experience. All the dental professionals concurred publications as the criteria for academic excellence.

  20. eIF4E、p-eIF4E和Mcl-1在病理性瘢痕组织中的表达和意义%Expression and significance of mRNA and protein of eIF4E、p-eIF4E and Mcl-1 in pathological scar

    Institute of Scientific and Technical Information of China (English)

    吴文艺; 张丽婷; 郑志芳; 朱世泽; 王朝阳

    2012-01-01

    Objective To study the expression of eIF4E,p-eIF4E (Ser 209) and Mcl-1 gene in the pathological scars and to investigate its role and its probable mechanism in the pathogenesis of abnormal scar.Methods Quantitative real-time PCR and Western Blot was performed to detect the expression and distribution of mRNA and protein of eIF4E and Mcl-1 in hypertrophic scar( 10 cases),keloid( 10 cases),normal scar( 10 cases),and normal skin( 10 cases).Western Blot was performed to detect the expression and distribution of protein of p-eIF4E in hypertrophic scar ( 10 cases),keloid ( 10 cases),normal scar( 10 cases),and normal skin( 10 cases).Results The expression of eIF4E mRNA and protein were 1.38 ± 0.45、1.23 ± 0.23 in the normal skin ( 10 cases) ; 5.400 ± 0.450、5.460 ± 0.460 in normal scar ( 10 cases ) ; 0.597 ± 0.060、0.590 ± 0.040 in hypertrophic scar( 10 cases) and 0.694 ± 0.066、0.697 ± 0.022 in keloid (10 cases).The expression of p-eIF4E protein in the normal skin (10 cases),normal scar( 10 cases),hypertrophic scar( 10 cases),and keloid ( 10 cases) were 0.202 ± 0.037、0.216 ± 0.019、0.426 ±0.026、0.433 ±0.027.The expression of Mcl-1 mRNA and protein were 1.510 ±0.660、1.400 ±O.530 in the normal skin (10 cases) ; 6.65 ± 0.85、7.23 ± 1.53 in normal scar( 10 cases) ; 0.589 ±0.059、0.660 ±0.063 in hypertrophic scar( 10 cases) and 0.870 ± 0.118、0.914 ± O.064 in the keloid (10 cases).The positive rate of mRNA and protein of eIF4E and Mcl-1 was not statistically different between the hypertrophic scar and keloid (P > 0.05 ),while they were all remarkably significant between normal scar and abnormal scar( P < 0.05 ).The phosphorylation of eIF4E in pathological scar was higher than that in control group.In pathological scar,mRNA and protein of eIF4E and Mcl-1 showed a strong positive correlation.Conclusions The result indicates that the expression of eIF4E、p-eIF4E and Mcl-1 is increased in pathological scar.eIF4E plays an important role in

  1. An expanded look at evaluating clinical performance: faculty use of anecdotal notes in the U.S. and Canada.

    Science.gov (United States)

    Hall, Mellisa A

    2013-07-01

    The evaluation of students in a clinical setting is often subjective and completed differently among faculty. To describe and compare evaluation practices, faculty were asked to rank order student clinical activities important enough to write an anecdotal note regarding. A total of 15 behaviors were presented and faculty were asked to prioritize their importance. Faculty were also asked demographic information regarding clinical teaching status. The frequency of writing an anecdotal note was requested, and if student notes were documented routinely or only at the end of the semester. After securing Institutional Review Board (IRB) review, ten percent of nursing programs were selected randomly using online data websites for listings of all public and private U.S. and Canadian nursing programs. A 23% response rate was obtained from the electronic survey (n = 849). A small percent of faculty reported the use of anecdotal notes only when they felt disciplinary action against a student was required, and eleven percent reported they were able to complete clinical evaluations without supplemental records. Describing faculty record-keeping practices provides greater understanding of nursing student evaluation. Nursing is the largest health profession in the world, and faculty accuracy in identifying clinical proficiency is vital to patient outcomes.

  2. Faculty Decisions on Serials Subscriptions Differ Significantly from Decisions Predicted by a Bibliometric Tool.

    Directory of Open Access Journals (Sweden)

    Sue F. Phelps

    2016-03-01

    Full Text Available Objective – To compare faculty choices of serials subscription cancellations to the scores of a bibliometric tool. Design – Natural experiment. Data was collected about faculty valuations of serials. The California Digital Library Weighted Value Algorithm (CDL-WVA was used to measure the value of journals to a particular library. These two sets of scores were then compared. Setting – A public research university in the United States of America. Subjects – Teaching and research faculty, as well as serials data. Methods – Experimental methodology was used to compare faculty valuations of serials (based on their journal cancellation choices to bibliometric valuations of the same journal titles (determined by CDL-WVA scores to identify the match rate between the faculty choices and the bibliographic data. Faculty were asked to select titles to cancel that totaled approximately 30% of the budget for their disciplinary fund code. This “keep” or “cancel” choice was the binary variable for the study. Usage data was gathered for articles downloaded through the link resolver for titles in each disciplinary dataset, and the CDL-WVA scores were determined for each journal title based on utility, quality, and cost effectiveness. Titles within each dataset were ranked highest to lowest using the CDL-WVA scores within each fund code, and then by subscription cost for titles with the same CDL-WVA score. The journal titles selected for comparison were those that ranked above the approximate 30% of titles chosen for cancellation by faculty and CDL-WVA scores. Researchers estimated an odds ratio of faculty choosing to keep a title and a CDL-WVA score that indicated the title should be kept. The p-value for that result was less than 0.0001, indicating that there was a negligible probability that the results were by chance. They also applied logistic regression to quantify the association between the numeric score of CDL-WVA and the binary variable

  3. [The Faculty Handbook: Agreement Between the County of Nassau and the Nassau Community College Faculty Senate.

    Science.gov (United States)

    Nassau Community Coll., Garden City, NY.

    This document presents the agreement between the County of Nassau and the Community College Faculty Senate. The agreement covers definitions, the faculty senate, work year, work week, work day, student advisement, maternity leave, sabbatical leave, leave of absence, outside activities and parttime employment, class size, overload, vacations,…

  4. Mathematics Turned inside out: The Intensive Faculty versus the Extensive Faculty

    Science.gov (United States)

    Grcar, Joseph F.

    2011-01-01

    Research universities in the United States have larger mathematics faculties outside their mathematics departments than inside. Members of this "extensive" faculty conduct most mathematics research, their interests are the most heavily published areas of mathematics, and they teach this mathematics in upper division courses independent of…

  5. Finding an Analytic Frame for Faculty-Student Interaction within Faculty-in-Residence Programs

    Science.gov (United States)

    Mara, Miriam; Mara, Andrew

    2011-01-01

    In this article we describe a case study analyzing how a Faculty-in-Residence program fosters student engagement. Using Cox & Orehovec's typology to add granularity to the National Study on Student Engagement's criteria for student engagement, we suggest best practices for the implementation of these in-situ faculty engagement programs.

  6. Faculty and student perceptions of the feasibility of individual student-faculty meetings

    NARCIS (Netherlands)

    Mulder, B.F.; Erich, M.H.; Borleffs, J.C.; Elgersma, A.F.; Cohen-Schotanus, J.

    2012-01-01

    The extent to which students feel involved in their education positively influences academic achievement. Individual student-faculty meetings can foster student involvement. To be effective, faculty acknowledgement of the benefit of these meetings is a prerequisite. The aim of this study was to expl

  7. The Influence of Nursing Faculty Workloads on Faculty Retention: A Case Study

    Science.gov (United States)

    Wood, Jennifer J.

    2013-01-01

    Nursing faculty workloads have come to the forefront of discussion in nursing education. The National League of Nursing (NLN) has made nursing faculty workloads a high priority in nursing education. Included in the priorities are areas of creating reform through innovations in nursing education, evaluating reform through evaluation research, and…

  8. An Exploratory Study of the Relationship between Online Instruction and Faculty Development among Teacher Education Faculty

    Science.gov (United States)

    Chaney, Kelly

    2010-01-01

    The purpose of the study was to explore factors that influence the intent of teacher education faculty members in the State of Illinois to teach online and their intent to participate in faculty development using Ajzen's theory of planned behavior. Understanding the beliefs and attitudes of teacher educators, their normative frame of reference,…

  9. Faculty Leadership in Baccalaureate Study Abroad Programs: The Relationship between Faculty Preparedness and Intercultural Competence

    Science.gov (United States)

    MacDonald, Carla J.

    2012-01-01

    The dissertation describes projected research to investigate whether a relationship exists between faculty in baccalaureate education who lead short term study abroad programs (SAPS) and their levels of intercultural competency. Specifically, the research collected considers whether a connection exists between those faculty who received…

  10. Faculty Motivation Toward Professional Improvement: A Study of Two-Year College Faculty.

    Science.gov (United States)

    Corwin, Luene Holmes

    Faculty from 16 food service and hotel technology programs in New York two-year colleges were surveyed to determine the components of faculty decisions concerning participation in professional improvement activities aimed at updating knowledge, to explore the function and relationship of the components of a composite expectancy model which…

  11. Faculty and Academic Environments: Using Holland's Theory to Explore Differences in How Faculty Structure Undergraduate Courses

    Science.gov (United States)

    Smart, John C.; Umbach, Paul D.

    2007-01-01

    Variation in how faculty in disparate theory-based academic environments design and structure their undergraduate courses to promote student learning in 12 areas was examined. The findings suggest that faculty create distinctive academic environments that reinforce and reward their preferred patterns of student competencies in a manner consistent…

  12. Faculty and Academic Environments: Using Holland's Theory to Explore Differences in How Faculty Structure Undergraduate Courses

    Science.gov (United States)

    Smart, John C.; Umbach, Paul D.

    2007-01-01

    Variation in how faculty in disparate theory-based academic environments design and structure their undergraduate courses to promote student learning in 12 areas was examined. The findings suggest that faculty create distinctive academic environments that reinforce and reward their preferred patterns of student competencies in a manner consistent…

  13. Quantitative evaluation of the requirements for the promotion as associate professor at German medical faculties.

    Science.gov (United States)

    Sorg, Heiko; Knobloch, Karsten

    2012-01-01

    First quantitative evaluation of the requirements for the promotion as associate professor (AP) at German medical faculties. Analysis of the AP-regulations of German medical faculties according to a validated scoring system, which has been adapted to this study. The overall scoring for the AP-requirements at 35 German medical faculties was 13.5±0.6 of 20 possible scoring points (95% confidence interval 12.2-14.7). More than 88% of the AP-regulations demand sufficient performance in teaching and research with adequate scientific publication. Furthermore, 83% of the faculties expect an expert review of the candidate's performance. Conference presentations required as an assistant professor as well as the reduction of the minimum time as an assistant professor do only play minor roles. The requirements for assistant professors to get nominated as an associate professor at German medical faculties are high with an only small range. In detail, however, it can be seen that there still exists large heterogeneity, which hinders equal opportunities and career possibilities. These data might be used for the ongoing objective discussion.

  14. Faculty's Academic Intellectual Leadership: Predictive Relations with Several Organizational Characteristics of Universities

    Directory of Open Access Journals (Sweden)

    Barış USLU

    2015-09-01

    Full Text Available The purpose of this research is to examine the predictive relations between faculty's academic intellectual leadership, and communication, climate and managerial flexibility regarding scholarly practices in universities. For this purpose, the research was designed in correlational research pattern, and, to collect data, an online questionnaire composed of Organizational Communication, Organizational Climate, Managerial Flexibility Regarding Scholarly Practices and Academic Intellectual Leadership scales was sent via e-mail to faculty who work in different disciplines in Turkish public universities. The questionnaires responded to by 504 faculties were included in the data analysis, and then descriptive, correlation and regression analyses were performed. According to the findings, Managerial Flexibility Regarding Service Practices is a significant predictor for all dimensions of academic intellectual leadership; Managerial Flexibility Regarding Teaching Practices for only the Guardian dimension; Supported Structurally, a dimension of the organizational climate, for Ambassador and Acquistor dimensions. This result shows that faculty's perceptions about climate in universities and the managerial support for scholarly duties strongly affect their academic intellectual leadership. Therefore, to enhance faculty's academic intellectual leadership behaviors, university managers can initiate different mechanisms such as learning-teaching centers, media advisory units and sporting-social event bureaus besides research-based facilities. University managers should also generate a more positive work environment by encouraging academics to follow their scholarly interests and recognizing academics' various achievements with material and moral rewards within the institution.

  15. The New Crisis in Academic Freedom Judicial Protection of American Public Faculty--- From the Debate between Public Speech and Public Employee Speech Caused from Garcetti Case%美国公立大学教师学术自由司法保护的新危机--从加西堤案引发的公言论与公职言论之争谈起

    Institute of Scientific and Technical Information of China (English)

    崔吉义; 杨海华

    2015-01-01

    In America,academic freedom has experienced a long period before obtaining the protection of federal constitution's first amendment.The center of judgment in the academic freedom case be-comes concentrated in distinguishing the property of public speech or private speech.The judgment of Garcetti case by the U.S.Supreme Court pushes public employee speech to the stage of history, which open a competition era of public speech and public employee speech.%在美国,学术自由在获得联邦宪法第1修正案保护之前经历了一个漫长的阶段,学术自由案件判决的重心逐渐集中于公私言论的辨别之上。联邦最高法院对加西堤案的判决,使公职言论登上了历史的舞台,从此开启了公言论与公职言论竞争的时代。

  16. EXPRSSION OF MCL-1,SURVIVIN AND BAG-1 IN HUMAN INTESTINAL METAPLASIA, DYSPLASIA, GASTRIC CANCER AND ITS CLINICAL SIGNIFICANCE%抗凋亡蛋白Mcl-1、Survivin和Bag-1在肠化、不典型增生和胃癌中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    李佳; 王东旭; 张辰新; 王津利

    2015-01-01

    目的 研究Mcl-1、Survivin和Bag-1在正常胃粘膜、肠化、不典型增生和胃癌中的表达,为寻找胃癌进展过程中的关键环节及靶向治疗提供方向.方法 应用免疫组织化学法(SP法)检测Mcl-1、Survivin、Bag-1在49例胃癌组织(GC)、24例癌旁不典型增生组织(DYS)、19例癌旁肠化组织(IM)、10例正常胃粘膜组织(NGM)中的表达.结果 Mcl-1在NGM、IM、DYS、GC中的阳性率分别为20.0%,21.1%,20.8%,46.9%;Survivin在NGM、IM、DYS、GC中的阳性率分别为10.0%,63.2%,66.7%,69.4%;Bag-1在NGM、IM、DYS、GC中的阳性率分别为10.0%,26.3%,33.3%,53.1%.胃癌中Mcl-1、Survivin的阳性率在有淋巴结转移组明显高于无淋巴结转移组,TNM分期为Ⅲ-Ⅳ期明显高于Ⅰ-Ⅱ期(P<0.05);Survivin、Bag-1的阳性率在低分化组明显高于高分化组(P<0.05).汇总49例胃癌中Mcl-1、Survivin和Bag-1蛋白的表达情况,其中28例有2种或3种蛋白表达,有淋巴结转移、TNM分期为Ⅲ-Ⅳ期的患者其有2种或3种蛋白共表达的比率(分别为70.0%,72.7%),明显高于无淋巴结转移、TNM分期为Ⅰ-Ⅱ期的患者(分别为36.8%,44.4%),P<0.05.结论 Mcl-1、Survivin可能与胃癌的发展有关,可能是胃癌预后不良的预测因子;Bag-l可能参与胃癌的发生,可能成为胃癌早期诊断的标志物之一.

  17. Cross-sectional online survey of research productivity in young Japanese nursing faculty.

    Science.gov (United States)

    Oyama, Yumiko; Fukahori, Hiroki; Miyashita, Mitsunori; Narama, Miho; Kono, Ayumi; Atogami, Fumi; Kashiwagi, Masayo; Okaya, Keiko; Takamizawa, Emiko; Yoshizawa, Toyoko

    2015-07-01

    To investigate the factors affecting the research productivity of young nursing faculty in Japan. An online survey targeting young nursing scholars (aged ≤ 39 years) who were members of the Japan Academy of Nursing Science was conducted from October to November 2012. Of 1634 potential respondents, 648 completed the survey (39.7%), and 400 full-time faculty of a baccalaureate degree program were selected for the analysis. The numbers of English-language and Japanese publications in the past 3 years were regressed onto personal characteristics, such as academic degree and type of university. The mean numbers of publications in English and Japanese in the past 3 years were 0.41 and 1.63, respectively. Holding a doctoral degree was significantly related to a higher number of publications in English and Japanese (e(β) = 5.78 and e(β) = 1.89, respectively). Working at a national university (e(β) = 2.15), having a research assistant (e(β) = 2.05), and the ability to read research articles in English (e(β) = 2.27) were significantly related to more English-language publications. Having the confidence to conduct quantitative research (e(β) = 1.67) was related to a larger number of Japanese publications. The lack of mentoring (e(β) = 0.97) and university workload (e(β) = 0.96) were associated with a lesser number of Japanese publications. The research productivity of young nursing faculty appeared to be quite low. Strategies to enhance research productivity in young nursing faculty, such as encouraging the achievement of a doctoral degree or enrichment of research resources, should be undertaken. © 2014 The Authors. Japan Journal of Nursing Science © 2014 Japan Academy of Nursing Science.

  18. Leading Change: Faculty Development through Structured Collaboration

    Directory of Open Access Journals (Sweden)

    Suzanne Painter

    2015-06-01

    Full Text Available There are relentless calls for innovation in higher education programs in response to media and policy-makers attention to such concerns as instructional quality, relevance to employment, costs, and time-to-degree. At the same time, the individual course remains the primary unit of instruction and there is little evidence of faculty development strategies to assist with changing core instructional practices. We faced that dilemma when we led an innovative doctoral program in educational leadership. Soon after beginning, we implemented a regular meeting of all faculty members teaching and advising in the program to address upcoming events and review student progress. Our retrospective analysis indicates that these meetings evolved as a practical and sustainable framework for faculty development in support of deep change for instructional practices. Here we describe the challenge of faculty development for change and draw lessons learned from our four years of leadership centered on experiential learning and community sense-making. We hope that program leaders who aspire to promote faculty development in conjunction with graduate program implementation will find these lessons useful.

  19. MCl_x(M=Pd, Fe, Cr) Assisted Synthesis of Ordered Mesoporous Carbon and Their Electrocatalytic Performance after Loading with Pt Nanoparticles%MCl_x(M=Pd,Fe,Cr)对有序介孔碳的辅助合成及其负载Pt后的电催化性能

    Institute of Scientific and Technical Information of China (English)

    孙盾; 何建平; 周建华; 王涛; 狄志勇; 王道军; 丁晓春

    2010-01-01

    MCl_x(M=Pd,Fe,Cr;x=2,3,3)为金属源,辅助合成有序介孔碳(OMC),以改善其负载Pt后的电催化性能.X射线衍射(XRD)和透射电镜(TEM)测试结果显示,适量PdCl_2的引入并未破坏介孔碳的有序结构,由于经历有机碳的高温裂解,OMC-PdCl_2主要以金属Pd为存在形式,较为均一嵌入OMC的骨架中,并在负载Pt的过程中与Pt形成二元催化剂.电化学氢吸附-脱附测试结果表明,Pt/OMC-MCl_x表现出优异的催化性能,电化学活性面积为Pt/OMC的2-4倍;其中Pt/OMC-PdCl_2最佳,活性面积达120.2 m~2·g~(-1),Pt/OMC-CrCl_3和Pt/OMC-FeCl_3次之.此外,Pt/OMC-MCl_x还具有良好的催化稳定性,经100个循环测试后,依然保持较高的催化活性,仅衰减22%-40%,使得该材料在催化领域具有很好的应用前景.

  20. ABT-737 resistance in B-cells isolated from chronic lymphocytic leukemia patients and leukemia cell lines is overcome by the pleiotropic kinase inhibitor quercetin through Mcl-1 down-regulation.

    Science.gov (United States)

    Russo, Maria; Spagnuolo, Carmela; Volpe, Silvestro; Tedesco, Idolo; Bilotto, Stefania; Russo, Gian Luigi

    2013-04-01

    Chronic lymphocytic leukemia (CLL) is the most frequent form of leukemia in adult population and despite numerous studies, it is considered an incurable disease. Since CLL is characterized by overexpression of pro-survival Bcl-2 family members, treatments with their antagonists, such as ABT-737, represent a promising new therapeutic strategy. ABT-737 is a BH3 mimetic agent which binds Bcl-2, Bcl-XL and Bcl-w with high affinity, while weakly interacts with Mcl-1 and Bfl-1. Previous studies demonstrated that quercetin, a flavonoid naturally present in food and beverages, was able to sensitize B-cells isolated from CLL patients to apoptosis when associated with death ligands or fludarabine, through a mechanism involving Mcl-1 down-regulation. Here, we report that the association between ABT-737 and quercetin synergistically induces apoptosis in B-cells and in five leukemic cell lines (Combination Index quercetin treatment. The molecular pathways triggered by quercetin have been investigated in HPB-ALL cells, characterized by the highest resistance to both ABT-737 and quercetin when applied as single molecules, but highly sensitivity to the co-treatment. In this cell line, quercetin down-regulated Mcl-1 through the inhibition of PI3K/Akt signaling pathway, leading to Mcl-1 instability. The same mechanism was confirmed in B-cells. These results may open new clinical perspectives based on a translational approach in CLL therapy. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. The anti-apoptotic protein BCL2L1/Bcl-xL is neutralized by pro-apoptotic PMAIP1/Noxa in neuroblastoma, thereby determining bortezomib sensitivity independent of prosurvival MCL1 expression.

    Science.gov (United States)

    Hagenbuchner, Judith; Ausserlechner, Michael J; Porto, Verena; David, Reinhard; Meister, Bernhard; Bodner, Martin; Villunger, Andreas; Geiger, Kathrin; Obexer, Petra

    2010-03-05

    Neuroblastoma is the most frequent extracranial solid tumor in children. Here, we report that the proteasome inhibitor bortezomib (PS-341, Velcade) activated the pro-apoptotic BH3-only proteins PMAIP1/Noxa and BBC3/Puma and induced accumulation of anti-apoptotic MCL1 as well as repression of anti-apoptotic BCL2L1/Bcl-xL. Retroviral expression of Bcl-xL, but not of MCL1, prevented apoptosis by bortezomib. Gene knockdown of Noxa by shRNA technology significantly reduced apoptosis, whereas Puma knockdown did not affect cell death kinetics. Immunoprecipitation revealed that endogenous Noxa associated with both, Bcl-xL and MCL1, suggesting that in neuronal cells Noxa can neutralize Bcl-xL, explaining the pronounced protective effect of Bcl-xL. Tetracycline-regulated Noxa expression did not trigger cell death per se but sensitized to bortezomib treatment in a dose-dependent manner. This implies that the induction of Noxa is necessary but not sufficient for bortezomib-induced apoptosis. We conclude that MCL1 steady-state expression levels do not affect sensitivity to proteasome-inhibitor treatment in neuronal tumor cells, and that both the repression of Bcl-xL and the activation of Noxa are necessary for bortezomib-induced cell death.

  2. Afatinib down-regulates MCL-1 expression through the PERK-eIF2α-ATF4 axis and leads to apoptosis in head and neck squamous cell carcinoma.

    Science.gov (United States)

    Liu, Xianfang; Lv, Zhenghua; Zou, Jidong; Liu, Xiuxiu; Ma, Juke; Wang, Jinhua; Sa, Na; Jing, Peihang; Xu, Wei

    2016-01-01

    Afatinib is the second generation of irreversible inhibitor of EGFR, HER2 and HER4, which has shown encouraging phase II and III clinical outcomes in the treatment of head and neck squamous cell carcinoma (HNSCC). However, the molecular mechanism of afatinib-induced apoptosis in HNSCC is poorly understood. In the present investigation, we discovered that down-regulation of MCL-1, an anti-apoptotic member of BCL-2 family, was responsible for afatinib-triggered apoptosis. And the inactivation of AKT-mTOR signaling caused by afatinib lead to translational inhibition of MCL-1 expression. As a crucial branch of ER stress, PERK-eIF2α-ATF4 axis was also stimulated in HNSCC cells after afatinib incubation. Silencing either eIF2α or ATF4 by siRNA transfection relieved afatinib-caused suppression of AKT-mTOR activity, attenuating MCL-1 down-regulation as well as subsequent apoptosis. Collectively, the results show that afatinib hampers AKT-mTOR activation by stimulating PERK-eIF2α-ATF4 signaling pathway, giving rise to MCL-1 down-regulation mediated apoptosis in HNSCC cells. Therefore, our findings reveal the elaborate molecular network of afatinib-induced apoptosis in HNSCC, which would provide substantial theoretical underpinnings for afatinib clinical application and highlight its promising prospect in HNSCC treatment.

  3. Nordic MCL3 study: 90Y-ibritumomab-tiuxetan added to BEAM/C in non-CR patients before transplant in mantle cell lymphoma

    DEFF Research Database (Denmark)

    Kolstad, Arne; Laurell, Anna; Jerkeman, Mats;

    2014-01-01

    The main objective of the MCL3 study was to improve outcome for patients not in complete remission (CR) before transplant by adding (90)Y-ibritumomab-tiuxetan (Zevalin) to the high-dose regimen. One hundred sixty untreated, stage II-IV mantle cell lymphoma patients maxi...

  4. Simulating Cl K-edge X-ray absorption spectroscopy in MCl62- (M= U, Np, Pu) complexes and UOCl5- using time-dependent density functional theory

    Energy Technology Data Exchange (ETDEWEB)

    Govind, Niranjan; De Jong, Wibe A.

    2014-02-21

    We report simulations of the X-ray absorption near edge structure (XANES) at the Cl K-edge of actinide hexahalides MCl62- (M = U, Np, Pu) and the UOCl5- complex using linear-response time-dependent density functional theory (LR-TDDFT) extended for core excitations. To the best of our knowledge, these are the first calculations of the Cl K-edge spectra of NpCl62- and PuCl62-. In addition, the spectra are simulated with and without the environmental effects of the host crystal as well as ab initio molecular dynamics (AIMD) to capture the dynamical effects due to atomic motion. The calculated spectra are compared with experimental results, where available and the observed trends are discussed.

  5. Quercetin-mediated Mcl-1 and survivin downregulation restores TRAIL-induced apoptosis in non-Hodgkin’s lymphoma B cells

    Science.gov (United States)

    Jacquemin, Guillaume; Granci, Virginie; Gallouet, Anne Sophie; Lalaoui, Najoua; Morlé, Aymeric; Iessi, Elisabetta; Morizot, Alexandre; Garrido, Carmen; Guillaudeux, Thierry; Micheau, Olivier

    2012-01-01

    Background Non-Hodgkin's B-cell lymphomas account for approximately 70% of B-cell lymphomas. While its incidence is dramatically increasing worldwide, the disease is still associated with high morbidity due to ineffectiveness of conventional therapies, creating an urgent need for novel therapeutic approaches. Unconventional compounds, including polyphenols and the cytokine TRAIL, are being extensively studied for their capacity to restore apoptosis in a large number of tumors, including lymphomas. Design and Methods Molecular mechanisms of TRAIL-resistance and reactivation of the apoptotic machinery by quercetin in non-Hodgkin’s lymphoma cell lines were determined by Hoescht, flow cytometry, Western blot, qPCR, by use of siRNA or pharmacological inhibitors of the mitochondrial pathway and by immunoprecipitation followed by post-translational modification analysis. Results Results demonstrate that quercetin, a natural flavonoid, restores TRAIL-induced cell death in resistant transformed follicular lymphoma B-cell lines, despite high Bcl-2 expression levels due to the chromosomal translocation t(14;18). Quercetin rescues mitochondrial activation by inducing the proteasomal degradation of Mcl-1 and by inhibiting survivin expression at the mRNA level, irrespective of p53. Restoration of the TRAIL pathway requires Bax and Bak but is independent of enhanced TRAIL DISC formation. Conclusions We demonstrate that inactivation of survivin and Mcl-1 expression by quercetin is sufficient to restore TRAIL sensitivity in resistant non–Hodgkin’s lymphoma B cells. Our results suggest, therefore, that combining quercetin with TRAIL treatments may be useful in the treatment of non–Hodgkin’s lymphoma. PMID:21933852

  6. Loss of PKCδ Induces Prostate Cancer Resistance to Paclitaxel through Activation of Wnt/β-Catenin Pathway and Mcl-1 Accumulation.

    Science.gov (United States)

    Flores, M Luz; Castilla, Carolina; Gasca, Jessica; Medina, Rafael; Pérez-Valderrama, Begoña; Romero, Francisco; Japón, Miguel A; Sáez, Carmen

    2016-07-01

    Prostate cancer is the leading cause of cancer-related death among men in developed countries. Although castration therapy is initially effective, prostate cancers progress to hormone-refractory disease and in this case taxane-based chemotherapy is widely used. Castration-resistant prostate cancer cells often develop resistance to chemotherapy agents and the search for new therapeutic strategies is necessary. In this article, we demonstrate that PKCδ silencing favors mitotic arrest after paclitaxel treatment in PC3 and LNCaP cells; however, this is associated with resistance to paclitaxel-induced apoptosis. In prostate cancer cells, PKCδ seems to exert a proapoptotic role, acting as a negative regulator of the canonical Wnt/β-catenin pathway. PKCδ silencing induces activation of Wnt/β-catenin pathway and the expression of its target genes, including Aurora kinase A, which is involved in activation of Akt and both factors play a key role in GSK3β inactivation and consequently in the stabilization of β-catenin and antiapoptotic protein Mcl-1. We also show that combined treatments with paclitaxel and Wnt/β-catenin or Akt inhibitors improve the apoptotic response to paclitaxel, even in the absence of PKCδ. Finally, we observe that high Gleason score prostate tumors lose PKCδ expression and this correlates with higher activation of β-catenin, inactivation of GSK3β, and higher levels of Aurora kinase A and Mcl-1 proteins. These findings suggest that targeting Wnt/β-catenin or Akt pathways may increase the efficacy of taxane chemotherapy in advanced human prostate cancers that have lost PKCδ expression. Mol Cancer Ther; 15(7); 1713-25. ©2016 AACR.

  7. Human Resource Development: A Model for Agricultural Faculty Members in Iran

    Directory of Open Access Journals (Sweden)

    Morteza Akbar

    2016-06-01

    Full Text Available Nowadays, there is an agreement among organizations that reinforcing of education leads to the improvement of organizational performance. The emphasis on the human capital in organizations reflects the view that market values depend less on tangible resources, but more on intangible ones, particularly human resources. The main purpose of this study was to design a model for faculty members in public Agricultural Higher Education in Iran. A survey method was used to collect data from nine Public Agricultural Faculties in Iran. Samples were collected from faculty members using a simple random sampling method (n=284 and a questionnaire was used as the main research instrument to collect data. Reliability and validity were calculated using Structural Equation Modeling through LISREL software, version 8.54 (above 0.7. Finally, a model was developed and tested for faculty members in Agricultural Higher Education System in Iran. The findings showed that contextual variables (i.e. personal, organizational, social, educational, and professional development had direct impacts on the process of Human Resource Development (HRD. Furthermore, personal and organizational development had direct and indirect impacts on HRD (R2 = 0.70.

  8. Humor in the classroom using faculty skits.

    Science.gov (United States)

    Smith, Cheryl Mixon; Noviello, Sheri Reynolds

    2012-01-01

    The infusion of humor in the classroom through faculty-developed skits is a teaching-learning strategy that engages nursing students in the learning process. Gardner's Multiple Intelligence Theory for Adult Learners provides the framework for the use of humor as a strategy in higher education. Three exemplars are presented with a description of the specific strategy, an objective for each strategy, and the effect of the strategy on student engagement in nursing education. In the exemplars, the authors provide "ready to use" ideas with some "pearls of wisdom" for other faculty interested in developing similar learning activities.

  9. Gerontological nursing. Faculty preparation for teaching.

    Science.gov (United States)

    Yurchuck, E R; Kee, C C

    1991-10-01

    1. As the number of older adults in the US increases, nurse educators must be prepared to teach gerontological nursing to their students. A 3-year project addresses the need for faculty development in gerontological nursing. 2. A major objective of the project is to provide basic knowledge of gerontological nursing to regional nursing faculty through a series of 1-week workshops that include didactic content and clinical observation experiences. 3. All 1990 workshops were filled to capacity, with participants exhibiting wide variation in their gerontological knowledge base.

  10. Factors Affecting the Involvement of Faculty Members in the Development of Instructional Materials: Basis for Policy Recommendations

    Directory of Open Access Journals (Sweden)

    Ryan V. Dio

    2015-11-01

    Full Text Available It is necessary for every higher education institutions to produce quality instructional materials (IMs with higher level standards that cater to the needs of the students and other stakeholders which will be part of the inventions produced by the institutions. This policy research is a descriptive method of study which involved 95 faculty of the Sorsogon State College chosen purposively as respondents. The study determined the profile of the faculty along IM preparation to serve as basis in recommending policy guidelines for the production and publication of instructional materials. Data were identified through triangulation of survey questionnaire, interview and documentary analysis. The study revealed that the faculty members advance studies, academic rank, and trainings attended are significantly related to their involvement in instructional materials preparations. Despite the fact that most of them do not have appropriate trainings on IMs preparation yet majority of the faculty are developing IMs which are utilized for instruction. However, very few are able to seek approval for its classroom utilization and very few have applied for copyright of their inventions so that these will be sold to the public for utilization and commercialization. To increase engagement of the faculty in the development of quality and publishable instructional materials, a scheme on policy recommendation has been formulated which contains provisions on the formation of the IMs committee, the evaluation procedures, approval, copyrighting and patenting, reproduction, publication and utilization of instructional materials.

  11. Public Education, Public Good.

    Science.gov (United States)

    Tomlinson, John

    1986-01-01

    Criticizes policies which would damage or destroy a public education system. Examines the relationship between government-provided education and democracy. Concludes that privatization of public education would emphasize self-interest and selfishness, further jeopardizing the altruism and civic mindedness necessary for the public good. (JDH)

  12. Faculty Library and Its Users (Interactively and Statistically

    Directory of Open Access Journals (Sweden)

    Rozalija Marinković

    2011-12-01

    Full Text Available According to popular opinion, statistics is the sum of incorrect data. However, much can be learned and concluded from statistical reports. In our example of recording statistics in the library of the Faculty of Economics in Osijek the following records are kept: user categories, the frequency of borrowing, the structure of borrowing, use of the reading room, use of the database, etc. Based on these data and statistical processing, the following can be analysed: the total number of users, the number of users by the year of study, by the content of used professional and scientific literature, by the use of serial publications in the reading room, or by the work on computers and database usage. All these data are precious for determining the procurement policy of the library, observing frequency of use of particular categories of professional and scientific literature (textbooks, handbooks, professional and scientific monographs, databases and determining operation guidelines for library staff.

  13. Making it Real: Faculty Collaboration to Create Video Content

    Directory of Open Access Journals (Sweden)

    Claudia Jennifer Dold

    2012-04-01

    Full Text Available Interest in integrative health care is a growing area of health practice, combining conventional medical treatments with safe and effective complementary and alternative medicine. These modalities relate to both improving physical and psychological well-being, and enhancing conventional talk therapy. In an interdisciplinary collaboration, teaching and library faculty have created a series of sixteen on-line video interviews that introduce practitioner-relevant experiences to students as supplemental course material. These videos are available through the department web-pages to students in other related disciplines as well, including Social Work, Counselor Education, Psychology, and the Colleges of Public Health, Nursing, and Medicine. The video series was undertaken as part of the educational mission of the library, bringing to the classroom new material that is essential to the professional development of future counselors.

  14. Making it Real: Faculty Collaboration to Create Video Content

    Directory of Open Access Journals (Sweden)

    Claudia Jennifer Dold

    2012-04-01

    Full Text Available Interest in integrative health care is a growing area of health practice, combining conventional medical treatments with safe and effective complementary and alternative medicine. These modalities relate to both improving physical and psychological well-being, and enhancing conventional talk therapy. In an interdisciplinary collaboration, teaching and library faculty have created a series of sixteen on-line video interviews that introduce practitioner-relevant experiences to students as supplemental course material. These videos are available through the department web-pages to students in other related disciplines as well, including Social Work, Counselor Education, Psychology, and the Colleges of Public Health, Nursing, and Medicine. The video series was undertaken as part of the educational mission of the library, bringing to the classroom new material that is essential to the professional development of future counselors.

  15. Measurement and comparison of nursing faculty members' critical thinking skills.

    Science.gov (United States)

    Blondy, Laurie C

    2011-03-01

    Nursing faculty members strive to teach students to think critically. It has long been assumed that nursing faculty members are good at critical thinking because they are expected to teach these skills to students, but this assumption has not been well supported empirically. Faculty members question their ability to think critically and are unsure of their skills. The purpose of this study was to address this assumption by measuring nursing faculty members' critical thinking skills and compare the faculty mean score to that of a student norming group, and to the mean scores of other nursing faculty studies. Findings can be used to increase nursing faculty members' understanding of their critical thinking skills, prompt discussion about critical thinking skills, and to help faculty members address concerns and uncertainty about the concept of critical thinking. This study also helps establish an empirical basis for future research.

  16. Mcl-1和Bax基因在2-甲氧基雌二醇诱导骨髓增生异常综合征细胞凋亡中的调控作用%Regulation of 2-Methoxyestradiol-induced Cell Apoptosis by Mcl-1 and Bax Genes in Myelodisplastic Syndrome

    Institute of Scientific and Technical Information of China (English)

    夏国华; 陈宝安; 芦慧霞; 邵泽叶; 丁家华; 高冲; 胡玲莉

    2009-01-01

    本研究探讨bcl-2基因家族成员调控2-甲氧基雌二醇(2-ME)谤导骨髓增生异常综合征(MDS)细胞凋亡的机制.用2-ME预处理MUTZ-1细胞,荧光比色法检测凋亡信号蛋白半胱氨酸蛋白酶-3(caspase-3)活性,用逆转录聚合酶链反应(RT-PCR)检测凋亡相关基因-髓细胞白血病基因-1(mcl-1)和bcl-2相关X蛋白(bax)mRNA的表达.结果表明:与时照组相比,2-ME增强MUTZ-1细胞内caspase-3活性,并呈浓度和时间依赖性(P0.05).结论:2-ME可能通过下调mcl-1表达和增强caspase-3活性的途径来调控MDS细胞凋亡.

  17. Faculty Power: Collective Bargaining on Campus.

    Science.gov (United States)

    Tice, Terrence N., Ed.; Holmes, Grace W., Ed.

    This document, an outgrowth of the national conference of the Institute of Continuing Legal Education held in 1971, sets forth the views of lawyers and educators concerning the legal, economic, and institutional implications of faculty collective bargaining. Part I, principles and practices of collective bargaining, discusses legal principles of…

  18. Sweet Briar College Faculty and Staff Handbook.

    Science.gov (United States)

    Sweet Briar Coll., VA.

    Statement of policies of Sweet Briar College, a liberal arts college for women, constitutes the main portion of its faculty handbook: academic freedom, appointment and reappointment, qualifications for appointment, promotion and tenure, termination or dismissal, leave policies, and benefits. A brief historical sketch, administrative organizational…

  19. The Radical Faculty -- What Are Its Goals?

    Science.gov (United States)

    Kampf, Louis

    According to radical faculty members and students, universities have been contradicting their humanistic educational ideals by concentrating on competitive professionalism and non-academic research in a struggle for institutional power in a preponderantly capitalistic society. It is their belief that meaningful education provides intellectual…

  20. Organizational Socialization: Processes for New Communication Faculty.

    Science.gov (United States)

    Cawyer, Carol Stringer; Friedrich, Gustav W.

    1998-01-01

    Surveys a national sample of new faculty members in communication departments to identify features of organizational socialization. Examines perceptions of socialization for the job interview and the orientation activities. Finds that amount of time spent in orientation activities is the best predictor of satisfaction upon arrival. (SR)